LOCUS       DAELSY010000001        80175 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.001, whole genome shotgun sequence.
ACCESSION   DAELSY010000001 DAELSY010000000
VERSION     DAELSY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 80175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.001"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            5..1015
                     /locus_tag="KPG09_000001"
     CDS             5..1015
                     /locus_tag="KPG09_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005213245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBG0964633.1"
                     /translation="MTLKTIREILPGLFISVLVGYISIFISILIPKVGAASISIFLGM
                     FVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFIILQMTIT
                     IIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGAIVPVVNATDKEKGIAVTIVNVTG
                     IFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLATIFKIVR
                     VIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALFSMNIIPK
                     EVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVSAVVLIKI
                     FI"
     gene            1309..2184
                     /locus_tag="KPG09_000002"
     CDS             1309..2184
                     /locus_tag="KPG09_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HBG0964634.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     RGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            2202..3692
                     /gene="cls"
                     /locus_tag="KPG09_000003"
     CDS             2202..3692
                     /gene="cls"
                     /locus_tag="KPG09_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG0964635.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            3885..4169
                     /locus_tag="KPG09_000004"
     CDS             3885..4169
                     /locus_tag="KPG09_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBG0964636.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            4202..5830
                     /gene="groL"
                     /locus_tag="KPG09_000005"
     CDS             4202..5830
                     /gene="groL"
                     /locus_tag="KPG09_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBG0964637.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVIVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            6092..6832
                     /gene="larB"
                     /locus_tag="KPG09_000006"
     CDS             6092..6832
                     /gene="larB"
                     /locus_tag="KPG09_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBG0964638.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            6916..7650
                     /gene="larC"
                     /locus_tag="KPG09_000007"
     CDS             6916..7650
                     /gene="larC"
                     /locus_tag="KPG09_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBG0964639.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            7680..8099
                     /locus_tag="KPG09_000008"
     CDS             7680..8099
                     /locus_tag="KPG09_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBG0964640.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKVTP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      8159..8260
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            8481..10016
                     /gene="guaA"
                     /locus_tag="KPG09_000009"
     CDS             8481..10016
                     /gene="guaA"
                     /locus_tag="KPG09_000009"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBG0964641.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTSITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            complement(10093..11139)
                     /locus_tag="KPG09_000010"
     CDS             complement(10093..11139)
                     /locus_tag="KPG09_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG0964642.1"
                     /translation="MPAYKDERRKSWYASFYFTDFVGDRKKKIKRGFKTKKEALEFER
                     EFLNKSKMSTDMSFESLIEEYMHDMSSRLKLSTLETKKYLINFRILPFFKSLKINEIT
                     ATHIRKWQNELLKSDYSQTYIKTINNQLVAVLNYAVKYYNLPSNPAHLAGTIGKKNAD
                     EMNFWTLEEFKKFIEFEKKPEPKLAFEILFWTGLRLGELLALTPKDIFENKISVEKSY
                     IKLNGEDIVSSPKTSKSKRVVPIPCFLYNNIKDYLSKLYDLKDNERIFKFAKSYLSKE
                     LNRCCKLSNVKRIRVHDLRHSHASLLVNMDVNILTIAERLGHEKVETTWNTYSHLYPN
                     KQLEVAQKLDNLNI"
     gene            complement(11215..11430)
                     /locus_tag="KPG09_000011"
     CDS             complement(11215..11430)
                     /locus_tag="KPG09_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0964643.1"
                     /translation="MLKKLRKKKRMTQLELAEKMGRNRSYISKLENQEYKDIGISTIL
                     DLSIALEEDFLELCKYYKFQEIKRRGK"
     gene            12084..12221
                     /locus_tag="KPG09_000012"
     CDS             12084..12221
                     /locus_tag="KPG09_000012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964644.1"
                     /translation="MGDSDCSKMLAFYEIMKMLETIDETKFYNCKEEIKSEFKHIKKE
                     D"
     gene            complement(12248..12640)
                     /locus_tag="KPG09_000013"
     CDS             complement(12248..12640)
                     /locus_tag="KPG09_000013"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0964645.1"
                     /translation="MYKDESNIFSKRLREEREELGLMQKEMANKLSLPANTYNGYETG
                     KRSPALDVVRHIADTLDISTDYLLGRTNIKINISNITEKELIKKLNPSDDMKEILDIF
                     SELDDDSKNKALKIAKLFLEEQNTKNKE"
     gene            12829..13023
                     /locus_tag="KPG09_000014"
     CDS             12829..13023
                     /locus_tag="KPG09_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008422227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0964646.1"
                     /translation="MKNYILKSLRVRQGLRQRDLAKILGMNPSTYSSKENGDRRFTVS
                     EAIKISDFFNTDLRDIFLNK"
     gene            13059..13850
                     /locus_tag="KPG09_000015"
     CDS             13059..13850
                     /locus_tag="KPG09_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBG0964647.1"
                     /translation="MNNLQVIEKNNERVLTTQQLADVYETDARNISNNFNNNKDRFIE
                     GKHYFLLQGDDLKHFKGIHTEYENLKFTSKMYLWTERGANRHCKILDTDKAWEQFDNL
                     EETYFKVKQHKPSCIEDVLIESLKEMKDLRLQVNRANNIALEAKAEVETIKDVVSLDS
                     NSWRTDTHQLIARIAKKQGGFEHINTLRTESYELLNKRFGVDLHRRLINKRRKMAEEG
                     VSESKREKVNNLDVIQDDKKLIEGYVAIVKDMALKYGISSDLSKN"
     gene            13942..14133
                     /locus_tag="KPG09_000016"
                     /pseudo
     CDS             13942..14133
                     /locus_tag="KPG09_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056356.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14137..14334
                     /locus_tag="KPG09_000017"
     CDS             14137..14334
                     /locus_tag="KPG09_000017"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964648.1"
                     /translation="MKISFESVVNNVNNETELILSKEELQIAKRILNILNENEQSILS
                     SKDILDFCKEALKYNLVPTFV"
     gene            complement(14321..14503)
                     /locus_tag="KPG09_000018"
     CDS             complement(14321..14503)
                     /locus_tag="KPG09_000018"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964649.1"
                     /translation="MQFSKDILHTLTLEILKKKYDFKNSSEEELLKHYHEIFLKLSEV
                     NNSFSKGDGLSVFKQT"
     gene            14574..14771
                     /locus_tag="KPG09_000019"
     CDS             14574..14771
                     /locus_tag="KPG09_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0964650.1"
                     /translation="MAINDNINKILRDRNIKAWKLAKEIGVDSGNLYSILRGKNKNPT
                     IDTLIKLADYLDVTLDELVGR"
     gene            14810..15019
                     /locus_tag="KPG09_000020"
     CDS             14810..15019
                     /locus_tag="KPG09_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964651.1"
                     /translation="MTKVVAKEQLFYRAKDVAKFLDICEATAYKIIAELNEELEKEGF
                     KTFSGRVSIAYFKERYCYKPRKGVI"
     gene            15121..15309
                     /locus_tag="KPG09_000021"
     CDS             15121..15309
                     /locus_tag="KPG09_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964652.1"
                     /translation="MRIIYKNKIYKVEQDKVLFRITYYDEQKNNRKFNNNKKVKRSVL
                     TRDIEVVNLYLPTHLKIK"
     gene            complement(15444..15623)
                     /locus_tag="KPG09_000022"
     CDS             complement(15444..15623)
                     /locus_tag="KPG09_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964653.1"
                     /translation="MINMSNKDIYTREEDKRFTLRINKLLFEKIEQLAQKDKRSVGRE
                     IEFILEKYFEDNPLE"
     gene            15754..15906
                     /locus_tag="KPG09_000023"
     CDS             15754..15906
                     /locus_tag="KPG09_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBG0964654.1"
                     /translation="MYDKDERVRFTFRLPATLLEKMKDIASKEGSSMNSLILHILWDY
                     IKEIEK"
     gene            15917..16132
                     /locus_tag="KPG09_000024"
     CDS             15917..16132
                     /locus_tag="KPG09_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964655.1"
                     /translation="MNKFDLKKVEVGNAVKVNCKRFGFEIDCIVVVATENELNLAYYD
                     KERGCMEYQALIVEDVRYGDYEILFLN"
     gene            16145..16315
                     /locus_tag="KPG09_000025"
     CDS             16145..16315
                     /locus_tag="KPG09_000025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964656.1"
                     /translation="MELAISVATSQIKQAKKNIKELKAKEDYDKDTLSWWEGVKQASE
                     NILEFLEIENKA"
     gene            16399..18351
                     /locus_tag="KPG09_000026"
     CDS             16399..18351
                     /locus_tag="KPG09_000026"
                     /inference="COORDINATES: protein motif:HMM:NF024867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0964657.1"
                     /translation="MKAILLKSLNIENFKGVKELHIDFNNVTNVFGENATGKTSIFDA
                     FTWVMFDKDSKNRSVFEIKPLDKQNKVIRGLVTTVTAVLEVNNKEIKLTKKYEEKWTR
                     KRGESEATFTKNETTYMINDTPIKKSEYVKDIAEIADEDQFKLLTNPYYFSNELNWKK
                     AREVILEICGDITTEQIIEAKQELTPLITEFEKENNIDKIIKNRKATKNNLSKEKEEI
                     PVRINECDKSMYSIDFEEIETQLNAKKVSLEVIEDELLSGTKANEQILKDKEKVFELK
                     QEIQSIKQSASERGNKKRNELINEKEGLKYNVKTLRNNLVYLEESRKLKENSRNKLVE
                     ETNNLRNEWSKKSQEVLDLNTIQTECPTCKRPLETSDIEVKKQEILDNFNLNKANVLK
                     EIGVLGKSKGEEVKKLNTEIEQLQLDEKEALKEIQEKAVLIDKIKKELENTKSTEVYS
                     LDEENRLVEIDAEIKELEEKINNKDDNKNIDELKENKKKLAVEIELLNKELAKRDINK
                     ELLDRKEQLLEKEKALGIELAHQEKILNLCELFIKTKVSLLESNISSKFKNVTFKLFK
                     EQINGGIEETCEALINGVPFSNVNTAGQINGGLDIINTLSNHFGVKMPIFIDNRESVN
                     TLIDIDSQVINLVVSNDNPLKIEGVN"
     gene            18354..19223
                     /locus_tag="KPG09_000027"
     CDS             18354..19223
                     /locus_tag="KPG09_000027"
                     /inference="COORDINATES: protein motif:HMM:TIGR00616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBG0964658.1"
                     /translation="MANEIQRQVSVKNLLSTEAYKKRFKEVLKDKANTFMASVVNVSN
                     LPSLKDAEPNSILKSAMVAATLDLPIDPNLGFSYLVPFTNKGVKEAQFQIGYKGFIQL
                     AMRTGQYKTINAIEIYEGEIKSVNRLTGEIEFNKNEDEIDKEIVVGYIAYFKLLNGFE
                     KTLYMSKEDMEKYAKRYSQTYKSNKDYVVKSSLWTTDFDAMAVKTVLKRLLSKYGILS
                     IEMQKALETDQAVIKDDNSVEYVDRQVEEEIEENANKKTIDMTTEEKEKAIEIKESKP
                     EEVKQQEFEAPPF"
     gene            19223..19936
                     /locus_tag="KPG09_000028"
     CDS             19223..19936
                     /locus_tag="KPG09_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0964659.1"
                     /translation="MKIKVLGSSSRGNCYLLQLKKETLILECGINYKDILKGLDFNLE
                     SVIGCLVTHEHKDHSKAITELTNNGIDVYSSKGTLEVLGIENHRTKVIESEKLFKIGN
                     FNIMPFSTKHDAVEPLGFLINHEEIGNLLFITDSYYCEYNFKNLNHIMIECNYSKDLL
                     DNNKNKIYLRNRIVKSHFELSNVINFLKANDLSNIKTITLLHLSEDNSDKDLFIKEIE
                     KNVGIPVIVAEKGLEIYLD"
     gene            20622..20753
                     /locus_tag="KPG09_000029"
                     /pseudo
     CDS             20622..20753
                     /locus_tag="KPG09_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            20800..21198
                     /locus_tag="KPG09_000030"
     CDS             20800..21198
                     /locus_tag="KPG09_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964660.1"
                     /translation="MELKFREWNKNGKEMYSYDEMVCYSKNLLREWVYSGVYLPTGNE
                     NSEVMIYTGLKDCIKKEIYEGDIVSYILSFEEFIGEVKFEEGFFVIDNEVLGECVGLF
                     HEIAVVKVIGNIYENPEMLEKIRKPKVLEG"
     gene            21201..21452
                     /locus_tag="KPG09_000031"
     CDS             21201..21452
                     /locus_tag="KPG09_000031"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964661.1"
                     /translation="MRFEIGKTYKFDKEKFIEINGVEHYKKYKELWIDNIEGVEFTVK
                     KIFENGYVHYPNEFYFAFGIVPEWCVEVKEVRGIRDDNN"
     gene            21439..21690
                     /locus_tag="KPG09_000032"
     CDS             21439..21690
                     /locus_tag="KPG09_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964662.1"
                     /translation="MIIIRSQDRLDLTRVNRVEISNNRVYAMFEDGIRKIGEYESNER
                     AIQVLNEIQKFIESGVKMDYIDSCRVRHNQEKVFEMPVK"
     gene            21707..22057
                     /locus_tag="KPG09_000033"
     CDS             21707..22057
                     /locus_tag="KPG09_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964663.1"
                     /translation="MAKVWVDAGTFLERTKDIGDIECNLREMINKNKQKNLIGRINSK
                     GQGRKSKKVECTNIITGESKIFVSAVEASKYLYFTELHITRLARTGKTTKNGWKVRYI
                     QEVTDGISKCGASN"
     gene            22026..22163
                     /locus_tag="KPG09_000034"
     CDS             22026..22163
                     /locus_tag="KPG09_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964664.1"
                     /translation="MVLANVEQVIKLAEKIINKKKCSVNKAIDIAIKILSRYEYEGMI
                     K"
     gene            22160..22618
                     /locus_tag="KPG09_000035"
     CDS             22160..22618
                     /locus_tag="KPG09_000035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964665.1"
                     /translation="MSLIEYRGYDFENKKWIYSKTIMWSNAVDCLLMLKEDFKWRKVC
                     NVGICSEEWARNNQEIYEGDILKEYDNFNDTSQYGVVKRDVGSIKIYLEWHYLKKFEG
                     EWIEIVNKTEIYHSRDYKVVGNEYENLEEVRKELLERKEKLENENTNAVN"
     gene            22593..22751
                     /locus_tag="KPG09_000036"
     CDS             22593..22751
                     /locus_tag="KPG09_000036"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964666.1"
                     /translation="MKIQMQLTKEKEFFKVYVNSEEEELEKLFYEFVSQMLACKRKNK
                     KVQGDIEK"
     gene            22748..22921
                     /locus_tag="KPG09_000037"
     CDS             22748..22921
                     /locus_tag="KPG09_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3797 domain-containing protein"
                     /protein_id="HBG0964667.1"
                     /translation="MKLKDIIKLGEKYCYCPSCGNDKIGNNEGKLIVEEHTYYRECKC
                     GFNILIDDRKDEI"
     gene            23040..23198
                     /locus_tag="KPG09_000038"
     CDS             23040..23198
                     /locus_tag="KPG09_000038"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964668.1"
                     /translation="MDDRLEICKKMFPNVTKNTRLLCHYCDGRNICSYDEEKARKLLR
                     EKEEVKND"
     gene            23191..23373
                     /locus_tag="KPG09_000039"
     CDS             23191..23373
                     /locus_tag="KPG09_000039"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964669.1"
                     /translation="MTNFEMIKNMSKKELAEFINEIGDNCTCIYCSCQYRYEICDVDE
                     KCVDGCKEWLDMEVEL"
     gene            23539..23760
                     /locus_tag="KPG09_000040"
     CDS             23539..23760
                     /locus_tag="KPG09_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964670.1"
                     /translation="MSKYVLRWQKGLLLDERKINYSCGSKEMLKQKAELLAKDDKILL
                     VTIDKVEEVIKDTRSQKMAEYFEDEGVEL"
     gene            23757..24755
                     /locus_tag="KPG09_000041"
     CDS             23757..24755
                     /locus_tag="KPG09_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG0964671.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVSQDMDIFFQKKINKVLR
                     DNDIRKAVFNDYSSNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNATLESCDLA
                     ICLYTEKDEKKVAILKLDYNKSYTHSISFEDDKFNIQMSKNEINIQETKAIKIGAIIG
                     LSGMNDEYHLKVLDKDAEKEEANSKFVTGFLNATKIKDDKFKTKMFKGLVDAFIANLY
                     SDMKQGEDVRNILLYMLREKQKLDINEFTDKAIKGDLKDSFKDHVEEKGLDGSFNIDK
                     KWVEKNLKNRHIKTDTGIEIKGKIDDFEDFMKYGIRHNGNGTVDIIIKNVSFYNEK"
     gene            24766..25056
                     /locus_tag="KPG09_000042"
     CDS             24766..25056
                     /locus_tag="KPG09_000042"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964672.1"
                     /translation="MKEQGRLVLNKLGTVDEVMKKVETDEKEINVYLKELKECEKAKV
                     LIIGKDGTEIPIVFKDETMNEWLRKEIEISFDEFKDEIVQVKQVLLKITGDN"
     gene            25076..25243
                     /locus_tag="KPG09_000043"
     CDS             25076..25243
                     /locus_tag="KPG09_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964673.1"
                     /translation="MTNKEMCKSKNITEKESYKRFSKEICKSCKNNKRDCESKDCDIT
                     YKNWLEDIYYV"
     gene            25236..25607
                     /locus_tag="KPG09_000044"
     CDS             25236..25607
                     /locus_tag="KPG09_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964674.1"
                     /translation="MYSTYKVKLKTKRTLEQVRNQSVDFEYSEEGLKNTLKYYNLIDS
                     LEVIVVKFGDEYCLVDCDEEDEKTIMEAYYLLEQDEYTGCYINEYELFKKDWENGSYY
                     GEVCMVFSDDEIEIIEKLREG"
     gene            25767..26108
                     /locus_tag="KPG09_000045"
     CDS             25767..26108
                     /locus_tag="KPG09_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HBG0964675.1"
                     /translation="MANIYCENYNCENYFEDMCMLERIEINNLKVCESYIEGKNELYK
                     LENGYTIHPKDLKMVKSKDYSVEVTHIPTGITVKCRSTNSILKNKNKCLEALEEELIK
                     INSHLELEDLF"
     gene            26233..26640
                     /locus_tag="KPG09_000046"
     CDS             26233..26640
                     /locus_tag="KPG09_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG0964676.1"
                     /translation="MKVNFTIDGEPIGKERPRFNLSTKRTYTPNKTRDYEDLIKWLYQ
                     SKVKHYFEGYIKMTLRCYYSIAKSNSKKVKEQKKNNVLRPSKKPDIDNVVKIVADALN
                     EIAYKDDTQIVEVVASKYYSDRPRIEVILEDVI"
     gene            26742..27596
                     /locus_tag="KPG09_000047"
     CDS             26742..27596
                     /locus_tag="KPG09_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HBG0964677.1"
                     /translation="MNENINKEITVLGTLEVEGMKFHDIEGGFGKGKKAMLVKDIAEI
                     HNRELKHINELINNNIKRFKTNIDIIDLKVSRSERLGDLNANPFEGLGYEAVGYSKQS
                     FNQSRNVYLLSERGYSKLLKILEDDVAWEQYEKIVDGYFSMRKELNNPLLSASKELQA
                     IFMLDKKQEVLETKIENVNEKLENFIDDAPLFNIECECIVKEVKRVATKSLGGHGSKA
                     YKNKSLRGKVYSDIYHQIKREFGVDSYKAIKRCQLNKVLEIVNSYKLPIVFEEEIRLL
                     NSQLSIVS"
     gene            27700..28206
                     /locus_tag="KPG09_000048"
     CDS             27700..28206
                     /locus_tag="KPG09_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964678.1"
                     /translation="MSKTKKEFFNATKKQLSNYKQLNANIIKLKNEIQMLKDNSVGDL
                     TKAISYDSVKTGKTNKTSNMIEDAIVNVSDLITEKEIELYEAEIIKSTIDLAIRNLKP
                     IHRQIIELKYIDGLMWQEMVDIIHLEERQLSVRASQAISSISIALFGKKALIEQEPLF
                     ELLDYKLN"
     gene            29053..29226
                     /locus_tag="KPG09_000049"
     CDS             29053..29226
                     /locus_tag="KPG09_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964679.1"
                     /translation="MATKSILKNVDVRKKAFGRNLVSALENAKNKQEKEVVLSKKCSE
                     VPKDKIKDIFRRF"
     gene            29226..29837
                     /locus_tag="KPG09_000050"
     CDS             29226..29837
                     /locus_tag="KPG09_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBG0964680.1"
                     /translation="MSGYSIVNLSNMLEELEEEEVKKILSSFSCPLNKDVEEFLKNKA
                     IEFSKQGLASTHLVLTSYKGKPVIIGYFSLANKYFTIKRKTLSNSLARKIVKFGQYNE
                     ELRRYIIGAPLIGQIGKNYSNNYNKLIKGDELLKIACDKIKAVQLDMGGKIVYLECED
                     KPKLIEFYKDNGFVDFGKRSLDKDETDSLDGDYLVQMLKYLKK"
     gene            29918..30163
                     /locus_tag="KPG09_000051"
     CDS             29918..30163
                     /locus_tag="KPG09_000051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964681.1"
                     /translation="MKKINDNLGFGIAFEEIKSSSKELGMRLPQWSEDVVIKIQRPDE
                     HSKMTASYLYVESRYGRVPWKETMIELFNEKWEVVEI"
     gene            30212..30469
                     /locus_tag="KPG09_000052"
     CDS             30212..30469
                     /locus_tag="KPG09_000052"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964682.1"
                     /translation="MNDYKEKTEPKLNILVQSKVDILCSNSSTMNGVDTSVYIKLSLD
                     NRVIGNLTNEDVKYEIIACFNECLEEINRKIGSEEVIDKLQ"
     gene            30508..31191
                     /locus_tag="KPG09_000053"
     CDS             30508..31191
                     /locus_tag="KPG09_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0964683.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWASE
                     KKKGAPKNKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETQDLINEIKNKDKFDIL
                     WEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENGEKIEYEFQFAWDRQASFLN
                     AQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVANLNKDDTDKEITVRV
                     MKASGENGK"
     gene            31181..32533
                     /locus_tag="KPG09_000054"
     CDS             31181..32533
                     /locus_tag="KPG09_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBG0964684.1"
                     /translation="MESKFISNDHFYDFIFNWDYKFYFLVGGYGSSKSYHVATKLIIK
                     LLEEKRKALVVREVYDTMRDSCFSLLEEVAESMGLGDILSFKTSPMRVIFPNGSKIIF
                     KGMDNPAKLKSINGVSIIWLEECSEVKYTGFKELLGRLRHPSLSNHIICSTNPIGEDN
                     WTYKHFFRDEEKDRFVLDDTVLYKNRIVIKNNTYYHHSIADDNFFLPESYIEQLDEMK
                     EYDYDLYRIARKGRFGINGTKVLPQFEVMEHQEVINKISNIPQRYYKVGFDFGFEKSY
                     NALLRLAIDHENKYLYIYWEYYKNQTTDDVTVKDIKEFKDTQELIKADAAEPKTIKYF
                     RQQGFNISGAKKYAGSRLQNTKKVKRFKKIYCSSECKHTIKELKNLTYATDKNGNLIY
                     DEFNIDPHTFSAIWYALDGYEVSDIKELKYNNDIYNKGLGLKKNNIPNQYNKKGGAVF
                     "
     gene            32533..34056
                     /locus_tag="KPG09_000055"
     CDS             32533..34056
                     /locus_tag="KPG09_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0964685.1"
                     /translation="MSIKNILLNLDEKELKDRKHAERDFLFYLGECRNKTMGLLDDDF
                     LGQSWITFDNLDYTPSQIIDNKVKPLINKQARFMFGKEPTIILKAYEKEDKETCEELR
                     QYIDSILNASKFWSNTLKAFKIATITKRVLLRLEAEPNQPIRLFYHSIDDFKYQVDSN
                     DITKLKSVIFVRFDASTIKEVTANQIWYRYTYYMKESSVNNQESCFLRVEKFKGDNLS
                     KPIEVKESDTKLSKIPCWIIVNEQSITNITGISDIEDLKPLQDTYNKRLSDFNDSLKF
                     LMFGQTVVVDATEETVNACKIAPNALMALKTLEEGSEKGKQAQAYRVESSFSNADPVD
                     SFFKRLEDSMYEKLAIPRPEQLQNIPSAKALKYLYTELIARCSEKWNDWEPAIRSMLR
                     LIVEACSKFNCYDDWNHDWDDLMFSIVLNKNYPIPEDEEDSKRLALEEVNNNVRSHRN
                     YIKEFGDDEDYEEAFNEVLEDNEKIQSVEQDQFRKDAEIEIDDIDEELNSKSNNSDE"
     gene            34069..35250
                     /locus_tag="KPG09_000056"
     CDS             34069..35250
                     /locus_tag="KPG09_000056"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964686.1"
                     /translation="MKDNTYTKKVLEARKKLLLLDKKIQLEILNVYKDASKIILNDIA
                     KNKELNLSTKYLKKLNKSIERYINELNQRLVPVAEKSVIEASNIAKELQMYYYQSIVP
                     NTAMNLACDAMCIKTTTGVVEKLVTGNFYKDKRSLDIRLWEYSSKNRKDIDRLIKANV
                     ARGANAKTLAKSLDNYVNPVRKTEAKTLEVGMNKSISYQAQRLARTSITHAFVETSVQ
                     NAINNPFCAGLQWNLSPQHYIRQVKWRGEDECDEYAEQNRFGLGEGVFPPEKYPIPHP
                     NCLCYPIQVIVPISEASKMMENWIGGGDNDTLDSWFDGIRHQNNSVPIIKKQRNKKNK
                     IATNSKDDRIKKNLTNSIKNKSKKAPKQVQKYINKYVQPSKILIDNSQKYLLFIILRL
                     I"
     gene            35403..35687
                     /locus_tag="KPG09_000057"
     CDS             35403..35687
                     /locus_tag="KPG09_000057"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964687.1"
                     /translation="MKNIENLQSIYTNSNELYNNEFISDILGALSNNEFEDLLATHSE
                     KYWSKNRNKEKEIFANLFTLKYQNNKEINSFIKEHLNDLDKIFNELLGGI"
     gene            35689..35841
                     /locus_tag="KPG09_000058"
     CDS             35689..35841
                     /locus_tag="KPG09_000058"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964688.1"
                     /translation="MLEEMRKDKKLLELRRLYKEKYGKNAPGFNYDEYNSYAEYKEKL
                     KELIQK"
     gene            35909..36562
                     /locus_tag="KPG09_000059"
     CDS             35909..36562
                     /locus_tag="KPG09_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBG0964689.1"
                     /translation="MLEYFKKLLGDEEGQKVYEKLSKDKENKLLLDNVKSPKYVEKTE
                     LENANKEIKEYKKQIGDRDKQLNDLQGKVKDNKELSDEIESLKNANKEIKENAEKEIE
                     VLKFNTALERAIESYNPKNAKALSALIDKENINFVDGKIIGLDEQIKAYQQSDSYLFN
                     NEKNKGDDENVGTGGLEGGTTSLLDNDNEISSIGELLADSKVKSENIEAQKKFFGEE"
     gene            36574..36930
                     /locus_tag="KPG09_000060"
     CDS             36574..36930
                     /locus_tag="KPG09_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964690.1"
                     /translation="MSIEKSEIYMGENKTILKFAGNLFQNINIKVKKTDVAVVDGKRI
                     LKAGTLISKDGKLVDGTTVTNDKAFGLVYRNIDFTHSNGNESIPVLIFGFVDEKTLPS
                     VIPEEAKQAMKMIMFL"
     gene            36954..37985
                     /locus_tag="KPG09_000061"
     CDS             36954..37985
                     /locus_tag="KPG09_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBG0964691.1"
                     /translation="MDWKDFIDSKEIAKYIKKLPLEMLIGESLFPRKKQIGMDLKYIK
                     GAKKKPVVLKQSTFDVAVKIRALKAQIEVKSKRMPFFKESVLVNEEDRQQLLLASKAQ
                     NKELLLMIISQIYDNYLALVDGGDMQMERMRMQALADGVINIVSEDGDLVFDFEVPSN
                     HKEVLTGSATWDNPDADIIGDIQRWMRIMRDEGKPLPKRMVMTSKTFGYFAKNKAIKL
                     DIDKDGRVILTDEMIKNYLKNKVGLSVAIVSGTYKLEDESEESYFPDNKITFIPDGDL
                     GKTYYGTTPEEADKVYGSKLDCSVVRTGIAITTMRLIDPVTVQTKVSQLGMPSFERAD
                     ECFFATVAS"
     gene            38000..38200
                     /locus_tag="KPG09_000062"
     CDS             38000..38200
                     /locus_tag="KPG09_000062"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964692.1"
                     /translation="MAKKKENLMQVKALVYLKYDNDCYKIDDIFEIRKADQELMEEKG
                     YIEVIGEAEEKNNNEILNKDGE"
     gene            38203..38520
                     /locus_tag="KPG09_000063"
     CDS             38203..38520
                     /locus_tag="KPG09_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964693.1"
                     /translation="MAINNLDKLKLNLQEEEYPYFTDEQLVMLLESNENNVLKASWRG
                     CLLKGATDDCIKIGPIETKSSNSSYWLSLADIYKTDYLEEKSKNETTNTGYKTSMTRV
                     DGQ"
     gene            38517..38900
                     /locus_tag="KPG09_000064"
     CDS             38517..38900
                     /locus_tag="KPG09_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964694.1"
                     /translation="MRKLRADKIIKTINRGIALNPQIITIEQEIKKIVDGAIEVTNEV
                     KELTVVIYPEKSNDTVVSSETIGTVYKNKNFGMVADKEADLRLNTENEITFKCIEGTM
                     KLNYVNPIVVEEKICGYICGLEKID"
     gene            38919..39323
                     /locus_tag="KPG09_000065"
     CDS             38919..39323
                     /locus_tag="KPG09_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964695.1"
                     /translation="MFTKAINEIDRKKATMPILCTNIAFMLEGEAKNNAKWTDRTGNA
                     RQGITGTSLGGGNQYIVRLGHGVDYGTVLEEGSAPHVIKPKSAKALFWNGASHPVKQV
                     QHPGTKGTHSLESTVSRNISKIGKLVEGHWSK"
     gene            39325..40149
                     /locus_tag="KPG09_000066"
     CDS             39325..40149
                     /locus_tag="KPG09_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964696.1"
                     /translation="MRAGIRKALIDNIPRLKDCYEPTVPNKKTIKPYIVVVQGEDADN
                     EGDVIGFRRTINIWLYEKRTTFNKLDELTKEVIETLDFKTITDDTSNEVFTCIYEGAV
                     GQDVIDEEWEAIIRCLRFSVIALDDKEDITNDRWVEALSKYTKNLLEIESYKDNWKKN
                     FIAPCALWRTTNIENKRINYHLIEITKTMKCHVVSKNKDEIVKLLEELETKLIIDKRV
                     RLREDKNMYLTLVSVVEDRESDMFSNGQLTVVFKMIGKIKREGPIMNKIYSNGNLK"
     gene            40162..40365
                     /locus_tag="KPG09_000067"
     CDS             40162..40365
                     /locus_tag="KPG09_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964697.1"
                     /translation="MAEAINKKTNISKQEEKYSKEDFLKNSEALGYKKEVVAGALFNC
                     KKDELTKVEFEKLIKDFLEREVK"
     gene            40367..41788
                     /locus_tag="KPG09_000068"
     CDS             40367..41788
                     /locus_tag="KPG09_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG0964698.1"
                     /translation="MATGAWNEKEKKDIPGFYNRFKTQAEKSTNTGLKGRLAMPIRAN
                     WGDVGKVITIKNDLRQLKTLFGDDMNYSAYKLGKLALLGNVKELLLYRLVDGNQKKGT
                     LTLKDTTENTAKDVIKLETKYPTSRNFNVTIKSNLVDSDKKDFIFFEGTKQLFSSSVK
                     GTIDEIVLEINSNLDNEYVIATKVADSDTTLANLVNVALEGGNDGCTSITNESYLKAL
                     EEFERYSFDSFVLDGVAEEALQETTKAWVAKNKELGKGILLFLGGKKEDNIKQINDKS
                     KSFNDENIANVGSSAYYENVKYTPSEVAVYIGALAVSKGITGSICNAKTIFEEVEPRL
                     SQSEVKECLKSGTLVLDFDDGDVIVVDDVNTFKKYVDDKNEAMGYISNIMFINTINKD
                     TSLKRKEFVGKIFNDATGQTTVICALKKYFEELMSQGIISEFNVDIDTELQATAKADE
                     FYWKWDAIKVDVMKKIYGTGYLG"
     gene            41804..42217
                     /locus_tag="KPG09_000069"
     CDS             41804..42217
                     /locus_tag="KPG09_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964699.1"
                     /translation="MYNDDYIEEASFLNGSDVVILIDGVEELYMEEIKADFEQDEQSI
                     KLLGCQNEISRVGTTKGSFSLSGYKVSSKFAKLGFKSFEIIYKLTNPQTLGYESIRLK
                     NCRLKKIPLINSKAGEDVKIELEGSYRGYDLLNEL"
     gene            42300..42788
                     /locus_tag="KPG09_000070"
     CDS             42300..42788
                     /locus_tag="KPG09_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964700.1"
                     /translation="MSEIYKRELENEVEDNDLDEEIEETNEDRLKMKEDEIIAKLLED
                     SPVPERTVFLDRLGIPITLKALTEKEISKIRKDCTKIVKVQGRREEKLNDDEFTLALI
                     EKGTVKPNFSNQKLLDAMKVTNAREFIKRKFLAGELSKISDQILELSGFYDEISDDDI
                     KN"
     gene            42965..47242
                     /locus_tag="KPG09_000071"
     CDS             42965..47242
                     /locus_tag="KPG09_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBG0964701.1"
                     /translation="MREIAKKEMYHIDVVISAKGDGETKSKLSTMEKYMKQTEKRMQT
                     LNRIKVNPAIKATDKASSVVNRVNNNMNKAKKTVTARIKATDNASPVANRASNNVNKA
                     KKTVTARLKATDNASSTVNKVNNKIKEVAKPVPPVIIRGQDESSSIIDKVKAKIQNLK
                     ADTIIKIKSQADEAINTISRTKNKLQEFVSKRYEAAVKIRDEASSALGGLTGKIDSFV
                     NGAISKFARLATTAGALIGGIGVGSAVKGFATFEQSMKNTQAVSGATGKEMEALTAKA
                     RQLGRETSFTAKDAGDAMYYMGMAGWKSEQMIKAIPDVLNLAAAGGTDLALTSDIVTD
                     GLTALGMTANDTTEFVDVMAATITNSNTSVELMGETFKYVGSMGGALGVSMKDLSLAT
                     GLMASASVKGSMAGTSLRGGLVRLIKPPEEAASAIKKYGIELKKNKNGSLDLAGTIGS
                     LREKLGGLEDVEKGVAISSIFGRTAMAGWAAVVNASESDFNKLTTAIAESEGEAKRIA
                     DMKLDTLSGQFEILKSAIDDVRISVGSKLGPMTRQFVEKLIKDMPKIGDAIVGVVEKF
                     VNNFDKIKAGFQVLLPAIGSVIASVMVLKATFAFGGAIKSLSLLANTFGTAKLAAFGL
                     TVGIGAIVIAFAGMTVAISNNKTAMMDLQTRFGSFGEYVTTIMETVGGVIKLTLGNLL
                     IMLGGIGKGIGILLSDKSWDEKASSLKNLFGKTTAEIKTNTKEALSDINGETSNATAL
                     LKKSTSKELQGVTKAFSTAFDQSKNVTEKKSNDIARALTNGLKGLDDQSLTMMRGLND
                     NMAIILSGVTADMKPSDKVNKITKNLDDAFKAGKLSAQEYRSSIQETLSFISKYSADS
                     SNNLKQGMSDAFNAFKEGTNVSGLKDGVTGMLNSLKATGPQALETLKGLGGKASEIFK
                     GVDFNSSIDAQKTKVLQNLNNLGLEGTQAIDTLRTIFSQASTALDTTNLKQGLSDTFN
                     SFKIGFNQDGINGAISNMLGTINQIGPQMQQTLGKMDGEMGQIFANVDFSTPIETQAS
                     KVLENLNNLGIEGPKALETVRSIFAQASSQIQGSASQTAQQANQQVANALTQENPAVQ
                     QAGQQLGTDLTNGVVNGVQTGVPTVQQKGNELGVATQQGVTDALNNATPQINNNNLTT
                     GIDTAFNQATATVQQGATNMYNGAKQSFSQLAQVGREAGSSLYNGATTSFNMLASNVR
                     VACSSMYNGARTSFTSLSSSAISAISAMCSSVVSQVSAMSSQIISYWNSVRATVSAPI
                     SASFNVKTTQTTVKRTVNEGGGILSNIFGRFADGGVASKPSICGEDGAEMVIPLSNNR
                     RNRAIGLYEQTGKMLGLSSTPSQKIGESYSNVVNNVRQFPLTNVLDTDNKEYREATPN
                     SVNSSNSTINLGGISINVQESNNKEEMIQEILSQVEDGLREALQDIG"
     gene            47324..48124
                     /locus_tag="KPG09_000072"
     CDS             47324..48124
                     /locus_tag="KPG09_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964702.1"
                     /translation="MWQKFKSLNTFLKVIIGIIAICFLPITLAVFSIEFLMKAIEAKN
                     KGKIVLGCFLALLTLSCARTVYLTKDLDDKNSTQVNQVSSNKDKEDAEKKAEEETKKQ
                     KEQKAQTEKQKKDEEAIKLVEQQKKNEDEQKSLENKKNNEPTKFFNDVYLKNKNCVNM
                     ASYEGIKSNIDIFGYKYKIIEPNETTLRQIDVYDNQSNDTVQFQFYENDTGEEVVTVM
                     QYNKKDEPDKSVAISDDFHIYPPKFNNHINNTNTEVNSIEEQINFMFN"
     gene            48193..48864
                     /locus_tag="KPG09_000073"
     CDS             48193..48864
                     /locus_tag="KPG09_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG0964703.1"
                     /translation="MVIDIYLKNEKEKIDFHFPVTPLDSLSIKKEKRFETVDIVNLGE
                     FDIKKEGEKIREISFKTFLPFQYDASYCRYSELKNPTEIVAMLEKWVDQSEPLRLIMT
                     GFGYNGLVTISSFSNTQTAGREEDRDIEITFRTYRELKIETLKKETKSNTKTDLKDNR
                     PNNKTTTKIYTVKASDTLYKIAKNLLGKGSRWPEIYNIPENKKTIGKNPNVIKKGQKL
                     VIPSK"
     gene            48861..49823
                     /locus_tag="KPG09_000074"
     CDS             48861..49823
                     /locus_tag="KPG09_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdQ"
                     /protein_id="HBG0964704.1"
                     /translation="MKIILNGKYDIANFNEGITLSEAIDGVAYKMDVSLVEPKQLQDI
                     GIKKGDKIVLIDIAYESKKEETIFNGVVWETRRSEKSKKLTLSCRERTVYMEESEEQY
                     QFKENTATQRIEYYCKQWNIPCYNLANTGKKLAKVIHKTNILDMIKKDLKETASKGGD
                     LFRVRMDNKLKLFKLGTNANVYKLDSILEDANFTSSFNDAVTSVKVLGKSKDENTKAP
                     IIGTYKKDSDKYGTLQKIKQDEKIKNAKEAKKAAEAMFNSGEETISVDCAVDINRIRA
                     GDKVSLKGKEYYVIDVTHTLDSIPKMKLNIGTLDYIRRKFYNND"
     gene            49816..50181
                     /locus_tag="KPG09_000075"
     CDS             49816..50181
                     /locus_tag="KPG09_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964705.1"
                     /translation="MTDARFNRVARILKEKMNKSVNDGVFGMGCSLAEITTNGLKVNG
                     YKDEIQDYLVLENLTLKEDYFTLSDEILGAEYRHKHKIETPKELKPLTIGDNVLVAVM
                     GTEFVVIGRVVNAKPISTK"
     gene            50156..50614
                     /locus_tag="KPG09_000076"
     CDS             50156..50614
                     /locus_tag="KPG09_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG0964706.1"
                     /translation="MPNLFPQNETFETVELKNNNENELDLKGSFLFDFIKGEFVKNAD
                     GTLKRCNKVEAYKQWCQKAILTPRYKRSAYSSVYGSEIKDLIASNLSQSAKELEISRL
                     IKETILVHPYTKEVSNFIFNWLENSRLVNYEFDVLTIDDENIVIDGNIKR"
     gene            50623..51759
                     /locus_tag="KPG09_000077"
     CDS             50623..51759
                     /locus_tag="KPG09_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG0964707.1"
                     /translation="MERELPIPVFLTEDEEVIHERMLSNFQDVSTLEGDFIYDATRPT
                     AEEITQLKQLGLQNNLKIAFPQTSYGTYLEWLGECKGVFKNQPTKSVGMVTFTGVQGA
                     IIAKGTIVTTIATDEKQSIEFELLETKTIGENETVDIKAECRIAGIIGNVSKGSISVL
                     LGSINGIKSVNNKEDFKGGTDIEDEEHFRERVLVSEQEDRLSGASSDYIRWAKEVDGV
                     GYAYVVSEWAGAGTVKVLILDKNRKAATQELIDKVQEYIYPLNVSEGENRDGKAPIGA
                     LVTVVTPETLLINVKASFIFSNSFSEETVLNNLKAKIDKYLDKIDLGGTVSYNAIQAI
                     VGSMMLTDEGIQDFSNLTINDVKENIKLQDQVVGVGEIVNEVVG"
     gene            51756..52403
                     /locus_tag="KPG09_000078"
     CDS             51756..52403
                     /locus_tag="KPG09_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012705217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBG0964708.1"
                     /translation="MIASKKGREMLLTLSPIYEQSIIMQSLYEAIGSEFDNLDLLNKE
                     IELQLFPQSATWGLEFWENRVGLSTNLDEAIEARRRKVIAKLQSKYIMTPKRMSMILQ
                     SYTGANIKINENISPYTFGVELTSTQGFPRDLEDLYKRVNVIKPSHLSVSYKLVSLLK
                     SKTYFAQTTIMSEEITVYPYSSKEVKANIKAKFALAHNMSSEILTVYPKGGGING"
     gene            52396..53196
                     /locus_tag="KPG09_000079"
     CDS             52396..53196
                     /locus_tag="KPG09_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG0964709.1"
                     /translation="MADEQFYTILTNIGKAKIANAGMLGKSVVLEKIQAGDGGGSYYN
                     PTEDQTALKNKVWEGNINVFDKDENNPNWIIATACVPGSIGGFTVREMALIDNEGDMI
                     AICKSPETYKPKVGNGAMKDLYLKFIIEVSNVEKVTLVVDPTAIFLTKKDEEKILTNI
                     NKLDTKIDTTKTELTSNIETAKTELNNKIGDTTQLTTTDKTNIVSALNEVKTSVDSIE
                     TTAEKTSYNNATSNLTATNVQGAIDEIVAKIENFNEINISIQNDMLPI"
     gene            53213..54931
                     /locus_tag="KPG09_000080"
     CDS             53213..54931
                     /locus_tag="KPG09_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964710.1"
                     /translation="MAIVYEFNYTGAEQSVVLPPGKYKFECFGACGGNYYDFVQCAKG
                     GYTAGSLILKENTTLHVYVGQSGYCKGVNGIETCRSGFNGAGGITTYKSTSDGYYSLA
                     GGGATDIRLIGGNWDNLQSLLSRIIVAGGGGGGSGNSHDSIGHGGGTKGKDGISIANK
                     YFAGGGSQFQGGLTFNSLYNGSFGVSGAGDGISGVGGGGGWYCGAGSFYAEFGGGGSG
                     YILTKDSYKPANYSPSSKYYFSDINSVVGGNTTKQDGYAKITLLQALPFLTISSYNST
                     TATFKADHTDPTLLTKIEYFIDDVLKETITTDLTTEKIINYTLEDNALHTLKIVVTDS
                     ANATAEKVVSISKGIAPLPAGSTTDEVTSKWIEIKDTFKSGKTSIINTLALKNIEASL
                     NNTLVELSEKIKTSFDSSDASVQDLMNQLTQANNTIAQLNTKYKVASGTVTPFKGDDT
                     KIAYPYLTDRVFKPSAWVKISNLDFKPNIFFADFDYYDTEYKNNYKLFLFACCGVATQ
                     RGVDFSSVTSFVRKSSDEYFHANGWLYNNSEGDVYFNNTGVQIPAYSFDSTQRHIYKW
                     YAIKFI"
     gene            54946..55242
                     /locus_tag="KPG09_000081"
     CDS             54946..55242
                     /locus_tag="KPG09_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964711.1"
                     /translation="MNVPNRVIYDQTGRIIFETGESCGDVLPHDKITELHYIDVEYGS
                     IDYTRNKIVGINIETKEPVLEEIPAYISKAEKEKQELENQLLLMTNKELGGGIL"
     gene            55242..55406
                     /locus_tag="KPG09_000082"
     CDS             55242..55406
                     /locus_tag="KPG09_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964712.1"
                     /translation="MNINNVVVRILAERILNKGLNPLKNRPFELDDVTNIEYRKAVDD
                     YINTIEQEIK"
     gene            55604..56215
                     /locus_tag="KPG09_000083"
     CDS             55604..56215
                     /locus_tag="KPG09_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964713.1"
                     /translation="MIEKLNENASLSDLITAFENSTKELKASKDNFTNLLGNPFLEST
                     KFSEFEGKMQNLMSTFKDNLKSKGINSGNTESLLSLINKVANIYIPTPIYTASGECTI
                     DNKGTCKRYTISTAGDVYYVRINLNFYPKFIVMYNSGGASKYFTTSYFCSYVPFIAST
                     VESYTTYLLDKSDSRIQVQKGTCILPIVYSKYGSSSFYFHAVG"
     gene            56227..56532
                     /locus_tag="KPG09_000084"
     CDS             56227..56532
                     /locus_tag="KPG09_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964714.1"
                     /translation="MNDYIEVGRRIFFDEEGEIIFYEGQSKGNVPERKNIKKIEYIDL
                     EYDYVDYDKYKIIGINIETKQPILEEIPIYMSEEEKRIQELENQLLIAENEKVGGLL"
     gene            56674..56937
                     /locus_tag="KPG09_000085"
     CDS             56674..56937
                     /locus_tag="KPG09_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964715.1"
                     /translation="MEQLLTELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVEMGAMKTDVEEIKEDVTDIKDMLQKEGL"
     gene            56938..57744
                     /locus_tag="KPG09_000086"
     CDS             56938..57744
                     /locus_tag="KPG09_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0964716.1"
                     /translation="MKVAIVPGHTLTGKGTGATGYINEGTENRILMDLVVKWLKKGGA
                     TVYSGKVDKSNNYLAEQCQIANKQNVDVAVQIHFNADHTTLDKMGTETIYKTNNGKKY
                     ADRVNKKLATVFKNRGAKSDVRGLYWLSHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKNIDNKENSEDKKMYKHTIVYDGEVDKIPATVVGWGYNDGKILICDIK
                     DYVPGKTENLYVVGGRACEKIGSMTKEKFTMIKGNDRFDTLYKALDFIKK"
     gene            complement(57771..57980)
                     /locus_tag="KPG09_000087"
     CDS             complement(57771..57980)
                     /locus_tag="KPG09_000087"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964717.1"
                     /translation="MKDVFEELISIKDACELYKKGESTLRLNIRNGKFVEGVDCKKFG
                     TTWVFKKTALEREYNKKDKKIESGN"
     gene            58129..58428
                     /locus_tag="KPG09_000088"
     CDS             58129..58428
                     /locus_tag="KPG09_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964718.1"
                     /translation="MKKITKKEIKKYVREAVNNNFNWEINKCGFYIKNNEIEFFISYK
                     GQGIDENVYNDTYEEIIYIEDIIEEYRRKEYNLEDVDSIVYDNVNDMINCYNEEK"
     gene            58517..59176
                     /locus_tag="KPG09_000089"
     CDS             58517..59176
                     /locus_tag="KPG09_000089"
                     /inference="COORDINATES: protein motif:HMM:NF013043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964719.1"
                     /translation="MKIGDKFENLTILDIEQKNSRKYCLCKCKNCGNEKWIRADSLKR
                     IKGCGCLQKETQFKQNDLTNKKFGRLTALKNTNKKAKSGHYIWICKCSCGNEIKTTEN
                     NLTTGRTKSCGCLKKESNIKNAKIALRVHQQKNIVDDTNISIIKKTEASINSKTKIRG
                     VCWNKEKGKYHAQIEFKKVHRNLGYYDNIEDAEKAYMEAKEKLHKKFLKDNDKLKEIE
                     Y"
     gene            59345..59821
                     /locus_tag="KPG09_000090"
     CDS             59345..59821
                     /locus_tag="KPG09_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964720.1"
                     /translation="MDKKEAKKFLKVVDMNIDKIKEEATKIYKECYLIETTDTIRISI
                     NLKGVVKSTISSWQGYSDDIFNMREIIVYEFSQGEVQIEDLLGELCFLNDYEEFATWC
                     DTESETLNWNSYEKFNKENFDELVERNIEDSLPDFLEELSESIENCKQELKSMIEI"
     gene            complement(59992..60291)
                     /locus_tag="KPG09_000091"
     CDS             complement(59992..60291)
                     /locus_tag="KPG09_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964721.1"
                     /translation="MDVEQSKNVYLRIGKELSEKMTKSELTKFSQEVANYLQYAGLDK
                     NMKIKLFQTIIEAVARKKVSLPAGSEFTQGIMLSNELEMTTNVGFIVEGLSYHNN"
     gene            complement(60542..60730)
                     /locus_tag="KPG09_000092"
     CDS             complement(60542..60730)
                     /locus_tag="KPG09_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964722.1"
                     /translation="MTKTILCNYCNKGIDKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHSYSKK"
     gene            60990..61139
                     /locus_tag="KPG09_000093"
     CDS             60990..61139
                     /locus_tag="KPG09_000093"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964723.1"
                     /translation="MEIVAVYYIAKFLLDLYKVKSHSKSEAKSGFIFNLTINKLIVKI
                     KYKKY"
     gene            complement(61373..61549)
                     /locus_tag="KPG09_000094"
     CDS             complement(61373..61549)
                     /locus_tag="KPG09_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964724.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(61604..61700)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            61911..62300
                     /locus_tag="KPG09_000095"
     CDS             61911..62300
                     /locus_tag="KPG09_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0964725.1"
                     /translation="MEQQEVVNKLRRGELMLMEYLWRKKSILSKKEIIEAMKDRYKWR
                     KSTTEILLKRLVKMKTLKKKRIGFELNYEVLVTKKEYLNARKEDRQVTKYDSFFIRMF
                     TTMHKKEKMTEEQIKKYIENMEKIGKE"
     gene            62380..62763
                     /locus_tag="KPG09_000096"
     CDS             62380..62763
                     /locus_tag="KPG09_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0964726.1"
                     /translation="MGFKKLPDSELKVMKFIWGLDKNKVASGEVVEAMKQIYDWNKNT
                     TLTILSKLAKKNYLYSQKTSKCTYYTIAVREKDYLKVETKKFFSFFHNNSLKSFFTAL
                     NDEEDLSDDKLDMLEQWVKNWEEDE"
     gene            62858..63010
                     /locus_tag="KPG09_000097"
     CDS             62858..63010
                     /locus_tag="KPG09_000097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964727.1"
                     /translation="MRFYQIKKKNNRMGIKYRMPEIHMNTGFEVSLTSILYWFDTINI
                     CQYLMD"
     gene            63442..64872
                     /locus_tag="KPG09_000098"
     CDS             63442..64872
                     /locus_tag="KPG09_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0964728.1"
                     /translation="MILNQIFIEDGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPRNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGEYISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKNNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(65020..65895)
                     /locus_tag="KPG09_000099"
     CDS             complement(65020..65895)
                     /locus_tag="KPG09_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0964729.1"
                     /translation="MINIRYLEILEAIEQTGTFTGAAKKLHITQSAVSHAIAELEQQA
                     ETSLFDRLPRGVCLTRCGISLLDEARSILTACRNLDKKISRLEECTPINIVSSITIAS
                     FLLPPILSRLKNSFPSLKVNVRVESANSAMDILKSGEADICFWEGIEPQGAFRTILLG
                     SYKLCVACAPTFPLSEQTLSLRQLCTFPLLLREQGSAIRDTLDSVLSLSNQKAYPVWE
                     SVNSFALIKAAEAGLGITILPENLLLDSLLHKKLRLIELDEIDMENKMLAILHKDKNI
                     TQPLEIILDNISNVL"
     gene            66015..66578
                     /locus_tag="KPG09_000100"
     CDS             66015..66578
                     /locus_tag="KPG09_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG0964730.1"
                     /translation="MNQSRKIYFWLLIINLFISTFTFGGGYVVVPMIRRYFVNQKQFF
                     TEDDLMSMAAVAQSTPGAIAINLSALAGYRVAGTLGVVISCTAAIIPPLVILGLVSAF
                     YTTFISNAIVTAVLKGMQAGVAALIVDLIIDMCSMILKQRSLFLSTMIPIAFFANFVM
                     SINVIFILSVCCFVCVMQVFYKKRENK"
     gene            66575..67141
                     /locus_tag="KPG09_000101"
     CDS             66575..67141
                     /locus_tag="KPG09_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG0964731.1"
                     /translation="MNTIWNLFFIFLKIGLFSIGGGYAIIPLIQEKVVNQTGWISEKM
                     FTDIITISQMTPGPLAVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKH
                     QNSTYIFEILNGLKSASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFV
                     ILLIVRKWKINPIVIIIISGILGIFFYL"
     gene            complement(67270..67506)
                     /locus_tag="KPG09_000102"
     CDS             complement(67270..67506)
                     /locus_tag="KPG09_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964732.1"
                     /translation="MLECFIAEDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(67816..71244)
                     /gene="uvrA"
                     /locus_tag="KPG09_000103"
     CDS             complement(67816..71244)
                     /gene="uvrA"
                     /locus_tag="KPG09_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG0964733.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFENELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFAKAINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLGNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKTPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPNAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPNKNYTALTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFTETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCNEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            71563..73797
                     /locus_tag="KPG09_000104"
     CDS             71563..73797
                     /locus_tag="KPG09_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0964734.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTASYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMDEILNIDSFNGEGTIEIINSQGKTLFIGKNNELIKDLSYKYKDSADEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDVVLGELNK
                     LNSFTIIMTFITTCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSNFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            74053..76083
                     /locus_tag="KPG09_000105"
     CDS             74053..76083
                     /locus_tag="KPG09_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0964735.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDLIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENEIILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDEDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            76080..76532
                     /locus_tag="KPG09_000106"
     CDS             76080..76532
                     /locus_tag="KPG09_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0964736.1"
                     /translation="MNKLVNVKCSFKIHANSKEEAIVSLVDVVSKEGYLIDKNQFLED
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDIPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            76534..77589
                     /locus_tag="KPG09_000107"
     CDS             76534..77589
                     /locus_tag="KPG09_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBG0964737.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLIGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     IGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            77656..77964
                     /locus_tag="KPG09_000108"
     CDS             77656..77964
                     /locus_tag="KPG09_000108"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBG0964738.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            78068..79333
                     /locus_tag="KPG09_000109"
     CDS             78068..79333
                     /locus_tag="KPG09_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBG0964739.1"
                     /translation="MKKLPIKTVVQRLLDLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKIADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLSVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVDKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKNEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            79421..80137
                     /locus_tag="KPG09_000110"
     CDS             79421..80137
                     /locus_tag="KPG09_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG0964740.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
BASE COUNT        32288 a         8568 c        14459 g        24860 t
ORIGIN      
        1 cagtttgaca ttaaaaacta taagggaaat attaccagga ctgtttatat cagtgttagt
       61 aggttatatt agtatattta tttctatttt aattcctaag gtaggggcag catcaatttc
      121 tatattttta ggtatgtttg taggaaacct atttttaaat caaaaggtat ttcaaaaagg
      181 ttataaattt tcagagacag atttgttatc atattcaata gtactattag gggcaacact
      241 cagtgtatct accttaatag acttaaaagt ttcaggaatt ttatttataa tattgcaaat
      301 gacaataaca ataatagctg cactttatat aggtaaaaaa ctaggatttg aagaaaactt
      361 tagatttcta atggcaagtg gaaatgcagt atgtggttct tctgccatag gagcaatagt
      421 tccagtggtt aatgcgacag ataaagaaaa gggaattgca gtaactattg taaatgttac
      481 tggtatattc cttatgtttt tacttccaat aatatctcaa tttttatata gccatgaact
      541 tgtaaaaact tcagcaatga taggtggaac tttgcaatca attggtcagg ttgtagcaag
      601 tggagcaatt gttggagaaa atgtaaaaga tttggctact atttttaaga tagtaagagt
      661 aatatttcta gttgttgttg tattagtatt tggacatatg aaaaatcagt ctaataggga
      721 aatccttgaa gaagaaaagg atgagataaa aaagaaaaaa gtaactatac catggtatgt
      781 aattggattc tttataactt gtgcactttt ttcaatgaac attataccaa aagaagtatc
      841 tgtgttgtgc aaagaaataa gtaacaaact agaaataata gctttagcag caattggatt
      901 aaaagttaat gtaaaagatt tagtgaaaca agggaaagaa gtttctttat atggtttatt
      961 tgtaggtaca gtacaagttg tatcagctgt agttttaata aaaatattta tatagcacaa
     1021 aaatagtatt tgtaatatta atttgattaa ttaaaataat atgatactaa taatttataa
     1081 tataaataaa aaatgaattt cgataagttt aagtgggtat aaacttatag aaattcattt
     1141 tttatttaag gtaaaaattt aaagtaaaat attattttac ataataataa ccttataaat
     1201 tttgaaatta cttatctttg tgttaaaata agtaaaatac acatattaaa gttaagtttt
     1261 taggcaaata aacaaaatga aaatataaat attaaggaga tttggtatat gaatgtttat
     1321 gaaaaaggta atggagttat actagaagga gtaacagatt ttgacccagt acatatattt
     1381 gagtgtggtc aatgttttag atggcacaaa caagaagatg gctcatacac tggagttgca
     1441 agggggagaa tattaaatgt caaaaaagag aatgataaaa tatatttaaa taatacgaat
     1501 ttaaaggagt ttaacagcat ttggtataat tactttgatt taggaactga ttatacagaa
     1561 ataaagaata agctaaaaaa tatggatgaa tatttgaata aggctactga atttggatgg
     1621 ggaataagaa tattaagaca agatggatgg gaaatgctta tatcatttat aatttcctct
     1681 aataatagaa ttcctatgat acaaagagcg atagaaaatt tatcaagaaa gtttggtaag
     1741 tatataggtg aatatgaagg taatgaatac tatgcattcc caacaccaga agaactaaat
     1801 aaggcctcac aagaggaaat aagagcatgt caaacaggtt ttagagataa gtacataaaa
     1861 agtactacac aggcagttat agagaataat gatgaggtat ctgaatatac aaatttaagt
     1921 acagaggatt gtagaaaaga gcttttaaaa tttaatggag taggaccaaa agtatgtgat
     1981 tgtattgcat tatttggtat gcaaaaatat gattcttttc cagttgatgt ttgggttaag
     2041 agagttatgc aagaatttta tatagatgaa gacatgagtt tacctaaaat gagaacttat
     2101 ggaatcgata aatttaaaga aatgtctgga tttgctcaac agtacttatt ttactatgca
     2161 agagaactcg gtataggaaa ataaattaag ggggagaaat tatgggagta ataggaacaa
     2221 tatttctgtt ctatttaatt atatcatatc tagctggagc cataatttct gtgattatat
     2281 tgcttgaaaa tagagaccct gcaaaaacaa tgtcttggct attgatgttt attatatttc
     2341 caggtgtagg tttaatgata tatgctattt caggtagaaa tattagaaaa agaaaattat
     2401 ttaagactca aaaactagct aataatataa aagaaaagaa actatttgat acattagaaa
     2461 agataacaga aatagttgaa ttagaaaaag aatctataaa acaaaataag cttttaagag
     2521 atgaagagga tggtagttat agaaaaaggg taataaatat gttgttaaag actggtatgt
     2581 ttccctttac taaaaataat aaagtggatg tatttgtaga tggaaatgaa aagtttaaaa
     2641 gacttataga agatataaga gaagctaaag accatattca tttagagtac tttatcatca
     2701 aagatagtga aataggtagg gtgttaaaag aagaacttat aaaaaaggct aaagaaggca
     2761 ttaagataag aattttatat gatgatgtag gatgctggag attttggttt aatagaaaat
     2821 ttttccgaga aatgagggaa gtaggtatag aaattgcagc atttttacct acaaaatttc
     2881 ctataatagg agggaaatta aactatagaa atcacagaaa aattgttgtt attgatggaa
     2941 taattggata tacaggtgga ataaatatag gagatgaata tttaggcaaa aatgataagt
     3001 ttggatattg gagagatact catattagga ttaagggtat ttctgtatac atgcttcaaa
     3061 tgacatttct gattgattgg tattatacga caaaagaagt attagtgact aaaaattatt
     3121 tcccaagtgt agggaatgtt ggtgaaagta tgatacaagt tgttgcgagt ggtccagaca
     3181 gtgactggga agatattcat tatgcttatt tttctgcaat atgtcaggct agaaaaaatg
     3241 tttatataga aactccatat tttattccag atgaaagtct actaaaagct ataaagagtg
     3301 ctgctcttag tggagttgat gtgagaataa tatttcctaa aattgcagac cacaagatag
     3361 taaatatagc atcttattca tattttgaag aaatattaag agcaggtggt aaagtttatt
     3421 tatataataa aggatttata cattctaaag tcgtaataat agatgataaa attgcttctg
     3481 caggtacagc taatatggat ttaagaagtt ttatgcttaa ctttgaggta aatgcattta
     3541 tctatgatga agaagttatt agggtaatga cagatgattt ctttgaggat ttaagtcact
     3601 gtgaagaact taatttggag gtatttaaga atagaaatat catacaaaaa atcaaagaat
     3661 ctgtagcaag attattttca ccaatattat aaaaaattat taaaaaaggt attgaaaatt
     3721 atgtgtgatt ggtatattat aataattgtt agcactctat tagatagagt gataataaat
     3781 aaaaagtata tatataaatc tacatatata agtagtatta atttaaaaat aactaaaaac
     3841 ataggcgaac tagtctatac ttaatatttt aggaggcgtg aataatgaaa ataagaccat
     3901 tagctgacag agtagtaatt aaaaaagtag aagcagaaga aaaaactgca agtggaatag
     3961 ttttaccagg agcagctaaa gagcaacctc aaatagctga agttgtagaa gttggaccag
     4021 gtggaatagt tgaagggaaa gaaataaaaa tggaattgac agtgggagat aaggttatat
     4081 tccaaaaata ttctggaaca gaagttaaga tagaaggaca agaatacaca atactaagac
     4141 agagtgatgt attagctgtt attgaataaa tatagaataa atttattagg agggatttaa
     4201 aatggctaaa gaaattaaat tttcagagga aacaagaaga gctttagaag ctggtgtaaa
     4261 taaattagca gatacagtaa aagtaacatt aggacctaaa ggaagaaatg ttatattaga
     4321 taaaaaattt ggttctccac ttataactaa tgatggagta actatagcaa aagagataga
     4381 actagaagat agatttgaaa atatgggtgc tcaattagtt aaagaagtag caactaaaac
     4441 taatgatgta gctggagatg gtactacaac tgctacagtt ttagcacaag ctataataag
     4501 agaaggttta aagaatgtaa cagcaggggc taacccaata cttttaagaa aaggaataca
     4561 aaaagcagta atagtagcgg tagaagaatt aaaaaatcaa tcaagaatag tagaaacaca
     4621 agaggctata tctcaagttg cttctatatc tgctggtgat gaagaagttg gaaaattaat
     4681 agcagaagct atggaaatag taggtaaaga tggagttata actgttgaag agtctcaaac
     4741 tatgaatact gaattagatg ctgttgaagg tatgcagttt gatagaggat ttgtttctgc
     4801 atatatggtt acagatgtag ataaaatgga agcagtttta aatgacccat atatattaat
     4861 tactgataag aaaatatcta acatacaaga attattacca gttcttgaac aaatagttca
     4921 acaaggtaaa aagttattga taatagctga agatgtagaa ggtgaagcat tatctacatt
     4981 agtagtaaat aaattaagag gaacatttga tgtagtagca gttaaagctc caggctttgg
     5041 agatagaaga aaagaaatgc ttcaagatat agcaatactt acaggtgctc aagtaatatc
     5101 agaagaatta ggttatgatt taaaagaagc tgatttatct atgctaggta gagcttcatc
     5161 tgttaaggta actaaagaaa gtactacaat agtagatggt tctggagata aaaaagctat
     5221 agaagataga gtaactcaaa ttaaacatca agtagagcaa actacttcag attttgatag
     5281 agagaaatta atggaaagat tagctaaact tgctggagga gtagctgttg taaaagttgg
     5341 agctgctaca gaagtagagt taaaagaaag aaaattaaga atagaagatg ctcttaatgc
     5401 aactagagca gctgtagaag aaggaatagt agctggtgga ggaactgctt ttgttagtgt
     5461 tataccagca ataggaacac taattgagag tttagaagga gaagttaaac taggtgctca
     5521 aatagttaaa aaagcgttag aagagccatt aagacaaata gctataaatg ctggtcttga
     5581 aggtgctgta attgtgcaaa atgttgttaa ttctgaagca gaaactggat ttgacgcatt
     5641 gaatgaaaaa tatgtaaata tgatagaagc tggtatagtt gacccaacta aagttagtag
     5701 aagtgcttta caaaatgctg catcaatagc aagtactttc ttaactactg aagctgctgt
     5761 tgctgacctt cctgaaaaag aagatgcagg aatgccagga atgggtggcg gaatgcctgg
     5821 tatgatgtaa tttaaaaaat ttaaaatata aattttaata taaagaacta tttcgactta
     5881 gattttaaac tctaagttga aatagttctt ttgtatatat ctaatttttg tatatctata
     5941 aattcaatac acatatttag ataatcagta tctagtttgt aataatattt tagagtagta
     6001 ttgttatttt ttaggttatg attttagata tatattaatt aaggtataat tgttgataga
     6061 gaataaatca aatttaaaag gatgcgataa tatggactta agaaaattac ttgaagaagt
     6121 taaaaatgaa aatattgata tagattttgc gttagaaaag ttaaaagatt taccttatga
     6181 agaccttgga tatgcaaata tagaccatca tagagaatta agaaatgggt atccggaagt
     6241 tatttattgt gaaggtaaga gtgatgagca tattttaggg attattgata aaatgaatca
     6301 aaaaagttct aacatattgg ggacaaggtg cagaaaagaa acttttctga aaattaaaga
     6361 aatctataat catgctgagt acgaagaggc gtctaaaatt cttaagattc aaaatcatga
     6421 tatagagaat ataggaaaag gtaaaattgt gattgctaca gggggaactt ctgatatacc
     6481 agtagcagac gaagcttatc atacagctaa gtttttaggt aatgatgttg atagaatata
     6541 tgatgttgga gtggcaggta tacataggct tttaaataaa agacataaaa tagatagtgc
     6601 tagagtaata gtagccgttg caggtatgga aggtgctctt gcaagtgtaa ttggtggact
     6661 ggttgatgtt ccagtaatag ctgttccaac ttctgttgga tatggagcaa attttggagg
     6721 attagcagca ttactagcta tgttaaatag ctgtgcatct ggaatatctg ttgtaaatat
     6781 tgataatgga tttggagcag gatatttagc atcaatgata aataaactgt aaatacattg
     6841 atataaatta aacaaaacta tataataaaa tttaatttct attatatttt tactattaaa
     6901 attgaggaga catatatgag tgaaaaaata ctttattttg atataataag cggtatatca
     6961 ggggatatga cccttgcaag tttattaaat ttaggtgtgc ctaaggaaat ttttttggag
     7021 gaaattcata aactaagaat gagcgatgaa tttaatataa acatcagctc gaaaactgaa
     7081 aatggaatag taggtacaaa agtagaagta attacaaaag aaaaacatac acatagaaat
     7141 ttagtggata tatttgaggt tatagataaa agcactctaa acgaaaatgt aaaatctaaa
     7201 gcaaaaaaaa tatttatggt aattggagaa gcagaagcaa aagttcatgg aactacaata
     7261 gacaagatac attttcatga ggtaggggct atagattcaa ttgtagatat agtaggagct
     7321 tctatattag ttgacttact ttgtgtagat aaagtttgtg ctagtacagt gtctgttggt
     7381 tcaggatttg taaaatgtga acatggaatt atacctatac ctgcacctgc aactatagaa
     7441 attttaaaag gtgtgccaat taaattaaat aatgtaaatg gagaatgtac aactccaaca
     7501 ggagctgcaa tcataaaagt cttgtgtgat gagtttgtag atgagcttga attttctcca
     7561 aagcaaattg gatatggtat aggtcataag aagtttgaag taccaaatat actcagaact
     7621 gtattaggtg aaaaaaaaaa gaagaattaa tttatgaaat tagtgcaaac attgatgata
     7681 tgtcttcaga aatctattct tatttgtatg aaaaaatatt agatgagaaa gcattggata
     7741 tttatacaga gagtatttat atgaagaaga atagaccagc aacaaaagta tcaattttat
     7801 gtaatgaatc agatttagaa aagtttatta aaatattact tttagaaact agtacttttg
     7861 gagtgagata taatgcatat aatagagaaa ttttagagag aaaatttgta aaattagata
     7921 caaaatatgg agtggttaca gtaaaactag ggtattataa aggaaaacta gtcaaggtta
     7981 ctccagaata taatgaatgt aaaactatag caaaaaatga agatattcct ataaaaaaag
     8041 tgtttgctga tataaatttt agaataaaag aagaatttag tgaaaactta ttgacataag
     8101 taataaattt tgataaatta atattcagcc ttattaaata aaagtaaaca gaaaaaaata
     8161 tataaaatat aaactcactc gtatatgctc agaaatatgg tctgagagtc tctaccaaga
     8221 taccgtaaat atcttgacta tgagtgaaat tattatacca aaagacctag ttaagtaaat
     8281 gtgtaggctg agttatactc cctatgtgtt ggctataaga taaaggtatt ttggaagcct
     8341 gtatagtaat ttcacttttg ctagaaatta tggaagttac tatacaggtt ttttgtgtaa
     8401 aaagatttaa tataatttag actgtattgt gttaaaataa atatataaaa actatattat
     8461 acaaggagag atgtgaattt atgaaacatg aattagtact tgtaatagat tttggaggac
     8521 aatataatca gttgatagct agaagagtta gagaaaacaa cgtatattgt gaaatacttc
     8581 catgtactgc aagcatagaa agaataaaag aaaaaaatcc taaagggata atatttacag
     8641 gcggtccaaa tagtgcgtat ttagaagatt ctccaacaat atcaaaagaa atatttgaac
     8701 taggagtacc aatacttgga atatgctatg gtattcaaat aatgtctcat gtattgggtg
     8761 gagttgtaag aaaaggtaat aaacaagaaa aggaatatgg aaaaacatct ataacttatg
     8821 gaaaatcatc attatttgaa ggaataacta caaatagtgt atggatgagt catacagatt
     8881 taatagaaaa agttccagaa ggatttacta tagttgcaaa tactaatgat tgtccagtag
     8941 cagctatgga aaatgtagaa cgaaacctat atggagttca attccatcca gaggtagaac
     9001 actgtttgga aggggacaag atacttacaa acttcttata taatatttgt aaagtaaaag
     9061 gtgactggac tacagattca ttcatagaag ataaaataaa agaattaaaa gaaaaaatag
     9121 gcgataaaaa agctttatgt gctttaagtg gaggagttga ttcttctgtt gctgcagttc
     9181 ttattcataa agcaattggg gacaacttaa cttgtatatt tgttgaccat ggtttactta
     9241 gaaaaaatga aggaaatgat gtagaaagaa tatttagaga gaaatttgat ataaatttaa
     9301 taagagttaa tgcagaagat agattcttat ctaaattaaa aggagtttcc gaaccagaag
     9361 ctaagagaaa aataataggt gaagaattta taagagtatt tgaagaagaa tcaaataaat
     9421 taggaaaaat ggatttctta gtacaaggaa caatctatcc agatgttata gaaagtggac
     9481 atggaaatgc tgctactata aagtctcatc ataatgttgg tggaatccca gaagatgtag
     9541 acttccaaga aattgtagaa ccattaagag agttatttaa ggatgaagtt agaaaaatag
     9601 gattagagct tggtatagaa gaaggattaa tatttagaca tcctttccca ggaccaggtc
     9661 taggaataag agttataggt gatgtaacta aagaaaaatg tgatatatta agagaagctg
     9721 atgctgttta tatggacgag cttagaaaag caggtttata cagagaaata tggcaagctt
     9781 ttgcaacatt accagatgtt aagactgttg gagtcatggg tgatgaaaga acatatgctt
     9841 atttagttgg tttaagagca gttacttctt ctgatggaat gacttctgac tggtataaga
     9901 tgccttatga tgtattagaa agaatttcta atagaataat aaatgaagtt gatggagtaa
     9961 atagagtagt ttatgatata acttctaaac ctcctggaac tattgaatgg gaatagtaaa
    10021 caaaaaagtc gtaagctttg tgatttcaag gcttgcgact tttttaaaaa tgcttatgat
    10081 actaaaatga tactatatat ttaaattatc tagtttttga gcaacttcaa gttgcttatt
    10141 cgggtataaa tgcgaatatg tattccaagt tgtttctact ttttcatgcc ccaatctttc
    10201 cgctatagtt aatatattta catccatatt cactaataac gacgcatgag aatgtcttaa
    10261 atcatgtact ctaattcttt ttacattaga taacttacaa catctattta gttctttaga
    10321 aagataactt ttagcaaatt taaatattct ttcattatct tttaagtcat acagcttaga
    10381 tagataatct tttatattat tatataaaaa acatgggata gggacaacac gtttactttt
    10441 agaagtttta ggagaagaaa caatatcctc accatttaat tttatgtaac ttttttcaac
    10501 acttatttta ttttcaaaaa tatcttttgg agtaagagct aataactccc ctagtctaag
    10561 acctgtccaa aataaaattt caaaagctaa tttaggttct ggtttctttt caaattcaat
    10621 aaattttttg aactcttcta atgtccaaaa attcatttca tctgcatttt ttttgcctat
    10681 tgtacctgct aaatgagcag gattagaagg taagttataa tatttaactg cataatttaa
    10741 tacagcaaca agctggttgt ttatcgtttt tatatatgtt tgactgtaat cactttttaa
    10801 taattcattt tgccatttcc gtatatgtgt ggcagtaatt tcatttattt ttaaactttt
    10861 aaaaaaaggt aaaattctaa agtttattaa atacttttta gtttctaatg tagataactt
    10921 taatctagaa gacatatcgt gcatgtattc ttctatgaga ctttcaaaac tcatatcagt
    10981 actcatttta gatttattta aaaattctct ttcaaactct agagcttctt ttttagtttt
    11041 aaaacctctc ttaattttct ttttcctatc cccaacgaag tctgtaaaat aaaagctagc
    11101 ataccaactt tttcttcgtt cgtctttgta agcaggcatt ttagacacct ccaattatgt
    11161 cattttatat tacaataatt ataacatgtt agatatgcta tctaaataat tgttttattt
    11221 tcctcttctt tttatttctt gaaacttgta atatttacac agttctaaga agtcttcttc
    11281 tagtgctata gataagtcta atatcgtaga tatacctata tctttgtact cttgattttc
    11341 cagctttgat atataacttc tgttacgtcc cattttttct gctaattcta gttgtgtcat
    11401 tcttttcttt tttcttaatt ttttcaacat ttattaaaat ccacctttta attattttta
    11461 tgacattttt ataattaata gtttgtgtaa tttcttgtaa aaaatgttcc tcgttagaac
    11521 attttttggt gggaaaatgt gttaaaatgt aagtaagaaa agcacattaa aagcactata
    11581 agaatagtgc tgagatgcta gagtttaaag aaagaataaa ttttatttaa aagcttataa
    11641 atataaagta aataatttgt aaattaaatc taagaataat gtaaaaaatg caataaaaaa
    11701 ataaaaatta aaattatgaa aaataaagct agccgactaa ctaaaaatag aaatatacaa
    11761 ataatctaaa atactagaaa taaaaaaata gaagtgaatt tttaaaatca aaactaaaat
    11821 ctctatatta gtcaagttgc aagataatat aaatttatat ataatatatt tacaaagaaa
    11881 atcttagaaa ttttaaatat tatatttgtt gcaataaata tatcaagtct ataagttaag
    11941 gaatttttat accatttttt acgaacataa gttctgaaaa gggatataaa atataatatt
    12001 ataaaaagaa ggtgattggc taaatttttt acatatttaa ggggcaagga ataaatatta
    12061 taaattataa gagggtgacg caaatgggag attcagattg ttctaaaatg ttggcatttt
    12121 atgaaattat gaaaatgtta gagactattg atgaaactaa attttataat tgtaaagaag
    12181 aaataaagtc agagtttaag catataaaaa aagaggattg aatcctcttt tttatatatt
    12241 tttatgctta ttctttattt ttagtatttt gctcctccaa aaaaagtttt gctatcttta
    12301 aagctttatt tttagaatca tcatcaagtt cactaaaaat atcaagtatt tctttcatgt
    12361 catcagatgg atttagcttt ttaataagtt ctttttctgt gatattagaa atattaattt
    12421 tgatattagt tctacctaat aaatagtcag ttgatatatc taaagtatca gctatatgtc
    12481 tcacaacatc aagcgctggg cttcttttac cagtttcata tccgttataa gtgtttgctg
    12541 gtaaacttaa tttattagcc atctcttttt gcattaatcc gagttcttct ctctcttctc
    12601 ttaatctttt agaaaatata ttgctttcat ctttatacat tgtaattgtc cccctcttaa
    12661 aaaactataa cgaatgtaat tactcttatt ctactacaaa atacgcattt tgtgatttta
    12721 attcgcaaaa aggaagaaaa aaacaaaaaa cgtattgaca attcacaaaa cggagtatat
    12781 tatataaata tagaaacttc aaattgagaa taaaaggagt gatatatatt gaaaaattat
    12841 atattgaaat ctcttagagt aagacaagga ttgagacaaa gagatttagc aaaaatatta
    12901 ggaatgaatc ccagcactta ctcaagtaaa gaaaatggcg ataggcgatt cacagtaagt
    12961 gaagcaataa aaatatcaga tttttttaat acagatttga gagatatttt tttaaataaa
    13021 taattcgcaa aatgtgaata aaaaggaggt agataaacat gaataactta caagtaatag
    13081 aaaaaaataa cgaaagagtt ttaactacac agcaactagc agatgtatat gaaacagatg
    13141 caagaaatat aagcaacaat tttaacaata acaaagatag atttattgaa ggtaaacatt
    13201 attttttatt gcaaggtgat gatttaaagc attttaaagg tattcataca gaatatgaaa
    13261 acctaaaatt tacttcaaaa atgtatcttt ggactgaaag aggagcaaac agacactgta
    13321 aaatattaga tactgataaa gcttgggaac aattcgataa tttagaagaa acatatttca
    13381 aagttaagca acataaacca tcttgcatag aagatgtttt gatagaaagt ttaaaagaaa
    13441 tgaaagattt aagactacaa gttaaccgag ctaataacat tgcattagaa gcgaaggcag
    13501 aagttgaaac aataaaagat gtagtttcat tagactcaaa tagttggaga acagatacac
    13561 atcaattaat tgcaaggata gcaaaaaaac aaggtggttt tgaacatata aacacgctta
    13621 gaacagaaag ctacgaatta ctaaataagc gatttggagt tgacctacat agaaggttaa
    13681 tcaataaaag aagaaagatg gcagaagaag gtgtatctga atccaaaaga gagaaagtta
    13741 acaatttaga tgtaatacaa gatgataaga aactcataga ggggtatgtt gctattgtaa
    13801 aagatatggc tttaaagtac ggaatatcaa gtgatttaag taagaattag gttaaataac
    13861 tgtagcactt tagaaattaa atacagaata ttttgaaata tattgtttta attaattatt
    13921 aactaggagg ttgttaaata catgaaaaat aatacaagtg attcaagagt aagatatttt
    13981 tgcaagtgtc catactgtgg gtttgataat gaggtagaag ttaaaaaagg attgaagcct
    14041 aagatatgtt gcatgtgtac aaaagaagtt gagtatgaaa aactggagca acaaagtgat
    14101 tcagaaaata tagaaattaa aggagtgtgt aattaaatga aaatttcatt tgaaagtgta
    14161 gtgaacaatg taaataatga aacagaatta atattatcaa aagaagagtt acaaatagct
    14221 aaaaggatac ttaatatatt gaatgaaaat gaacaaagta tcctttcctc aaaagatatt
    14281 ctagattttt gcaaagaagc tcttaaatat aatttagtgc ctacgtttgt ttaaagacac
    14341 tgagaccatc acctttagaa aaactattat tgacttctga aagtttcaaa aagatttcat
    14401 ggtaatgttt taaaagttct tcttcagaag aatttttaaa atcatatttt ttcttaagaa
    14461 tttctaaagt aagagtatgt aatatatctt tactgaactg cataatatca ccaactttca
    14521 taaaaagata taggatatat cctacaaata tattatatca aaggaggaaa ttaatggcaa
    14581 ttaatgacaa cataaataaa attttaagag atagaaacat aaaagcatgg aaattagcaa
    14641 aagaaatagg cgtagattca ggaaatttat attcaatttt aagagggaaa aataaaaatc
    14701 caacgataga tacattgata aaactagctg attatttaga tgttacatta gacgaattag
    14761 ttggaagata aaaattaaat acagaatatt ttgaaagtag gtgttttaga tgacaaaagt
    14821 agtagctaaa gaacaattat tttatagagc aaaagatgta gctaaattct tagatatatg
    14881 cgaagcaact gcatacaaaa taattgcgga attaaatgaa gaattagaaa aagaaggttt
    14941 taagactttt tctggcaggg tatcaattgc ttatttcaaa gagagatatt gctataagcc
    15001 aagaaagggg gtgatttaat tgaatgtaaa ggtactaata gcttatatac agttttgtaa
    15061 gcaatataat aagaaagcaa gttttgaagg tcttaaaaaa tacaacaaag gggtaattgc
    15121 atgagaataa tttataaaaa caaaatctac aaagtagaac aagacaaagt gttatttaga
    15181 attacatact atgatgagca gaaaaataac aggaagttta ataataataa gaaagtaaaa
    15241 agaagcgttt taacaagaga tatagaggta gttaacttgt atttaccaac acatttaaaa
    15301 ataaagtaag ctataaataa ttaaagaaaa aggtgattag atggaaatag aacaaacaac
    15361 aatacgcctg tctagagaac ttaaagacaa gcttctaaaa caggcaaaag ttaaaggtta
    15421 tacgctaaaa gatatggtag tttttattct aaaggattat cttcaaaata tttctcaaga
    15481 ataaattcaa tttctctacc cacagaacgc ttatcttttt gagcaagttg ttctattttt
    15541 tcaaaaagaa gtttattaat tcttagtgta aatctcttat cttcttcacg agtataaata
    15601 tctttattag acatatttat cattccttaa tacaaaattt gacgtcttta tgacacttta
    15661 ataataaaat aattaatgaa gaaagtcaat aaaaatgctt gacgtcaaat gtatgacatg
    15721 gtattattaa aacaagaaag gggttgacgt caaatgtatg acaaagacga gagagtaagg
    15781 tttactttta gattaccagc aacattactt gaaaaaatga aagatatagc atcaaaagaa
    15841 ggaagttcta tgaactcgct aatattgcac atactttggg attacataaa agaaatagaa
    15901 aaataaggag gtaattatga ataaattcga tttaaagaaa gttgaagtag gaaatgcagt
    15961 aaaagtaaat tgcaaaagat ttggttttga aattgattgc attgtagtag tagcaacaga
    16021 aaatgagcta aatttagctt actacgacaa agaaagagga tgtatggagt atcaagcatt
    16081 aatagtagaa gatgttagat atggtgatta tgagatttta tttttaaatt agggggtgta
    16141 tttgatggaa ttagctatat cagttgcaac aagtcaaatt aaacaagcaa agaaaaacat
    16201 aaaggaatta aaagcaaaag aggattatga caaagacact ctaagctggt gggaaggtgt
    16261 taaacaagct agcgagaata tactagagtt tttagaaata gaaaataaag cataagaaaa
    16321 gagcctaggg tgaggctcta atcaaaaaaa caaaaaataa atttaataat atgtattata
    16381 acataagggg gaactaaaat gaaagcaatt ttattaaaaa gtttaaatat agaaaatttc
    16441 aaaggagtaa aagagcttca catagatttt aacaatgtaa ctaatgtatt tggagaaaat
    16501 gcgactggta aaacaagtat atttgatgcc tttacgtggg taatgtttga taaagatagt
    16561 aagaatagaa gtgtatttga gataaagcct ttagataaac aaaacaaagt cattagagga
    16621 cttgtgacga ctgttacagc agtattagaa gttaataata aagagataaa gttaactaag
    16681 aaatacgaag aaaaatggac tagaaaaaga ggagaatcag aagcaacttt tactaaaaat
    16741 gaaacaactt acatgataaa tgatactcca ataaaaaaga gtgaatatgt aaaagatata
    16801 gcagaaatag cagacgagga ccagtttaaa ttgcttacca atccttatta tttttcaaat
    16861 gaacttaact ggaaaaaagc tagggaagtg attttagaga tatgtggaga tataacaaca
    16921 gaacaaatta tagaagctaa acaggagcta actccactta ttacagaatt tgaaaaggaa
    16981 aataacatag ataagattat taagaataga aaagctacta agaataatct atctaaagaa
    17041 aaagaagaaa taccagttcg tataaatgag tgtgataaaa gcatgtacag tatagatttt
    17101 gaggaaatag aaactcaact taatgctaaa aaagttagct tagaagttat cgaggatgag
    17161 ttactgagtg gtacaaaggc aaatgagcag attttaaaag ataaagaaaa agtatttgaa
    17221 ctgaaacaag agatacaaag tattaaacaa tcagcgagtg aaagaggaaa taagaagaga
    17281 aatgagttaa taaatgaaaa agaaggttta aaatataatg tcaaaacatt aagaaataac
    17341 cttgtatatt tagaagaaag cagaaaacta aaagaaaatt cgagaaataa actagttgaa
    17401 gaaacaaata atctgagaaa tgaatggtct aaaaagagcc aagaggtatt agatttaaat
    17461 accatacaaa cagagtgtcc aacttgtaaa agacctctag aaactagcga tatagaagtt
    17521 aaaaaacaag aaatactaga taattttaat ctaaataaag caaatgtatt aaaagaaata
    17581 ggagtattag gaaaatctaa aggtgaagaa gtaaaaaaac ttaatacaga aattgaacag
    17641 ctacaactag atgaaaaaga agccttaaaa gagatacaag aaaaagcagt actaatagat
    17701 aagataaaaa aagagttaga aaacacaaaa tctacagagg tatactcttt agatgaagaa
    17761 aatagactgg ttgaaattga tgcagaaata aaagaattag aagaaaaaat aaacaataaa
    17821 gatgataata aaaacattga tgaactaaaa gaaaacaaga aaaaactagc tgttgaaatt
    17881 gagttactta ataaagaact agctaaaaga gatattaaca aagaactatt agatagaaaa
    17941 gagcagttgt tagaaaaaga aaaagcgtta ggtatagaat tagctcatca agaaaaaata
    18001 ctcaacttat gtgagttatt tataaaaact aaagttagtt tattagaaag taatataagt
    18061 agtaaattca aaaatgtaac atttaagtta ttcaaagaac aaataaatgg aggcattgaa
    18121 gaaacttgtg aagctttaat aaatggtgtg cctttcagca atgtcaatac ggcaggacag
    18181 ataaatggag ggctagatat aataaacact ctatctaatc attttggagt taaaatgcca
    18241 atattcattg ataacaggga aagcgtgaat actttgattg atattgatag ccaagtgata
    18301 aatttagttg taagtaatga taatccatta aaaatagaag gagtgaacta aacatggcaa
    18361 atgaaataca aagacaagta agtgttaaga atttactttc tacagaagct tataagaaaa
    18421 ggtttaaaga ggttttaaaa gacaaggcta atacatttat ggcatcagtt gttaatgtat
    18481 ctaacttacc aagtttaaaa gatgcagaac ctaattcaat tttaaaatcc gctatggttg
    18541 cagcaacatt agacttacca atagacccga atttaggctt ttcttactta gtacccttta
    18601 caaataaagg agttaaagaa gcacaatttc aaattggtta taaagggttt atacaactag
    18661 ctatgaggac tggacagtat aaaacaatta atgcaataga aatctatgag ggtgagatta
    18721 aaagcgttaa taggctgact ggagagatag agtttaataa aaatgaagat gaaatagaca
    18781 aagagattgt tgttggatat atagcatatt ttaaattgtt aaatggattt gaaaaaactc
    18841 tatacatgag taaagaagat atggagaaat acgctaaaag atatagccag acatacaaaa
    18901 gtaataagga ttatgttgta aaatcaagtc tttggacaac tgactttgat gctatggcag
    18961 ttaaaacagt acttaaaaga ttgctttcta aatacggaat attaagtata gaaatgcaga
    19021 aagctttaga aactgaccaa gcagttataa aggatgataa ttcagttgaa tatgtagaca
    19081 gacaagtaga agaagaaata gaagaaaatg ctaataaaaa gactatagat atgacaacag
    19141 aagaaaagga aaaagctatt gaaattaaag aatctaagcc tgaagaagta aagcaacagg
    19201 aatttgaagc accaccgttt taatgaaaat taaagtgtta gggagcagta gcagaggtaa
    19261 ttgttacttg ctccaactaa aaaaagaaac attaatactt gagtgtggaa ttaactataa
    19321 agatatatta aaaggcttag atttcaattt agaaagtgtt attgggtgtt tggtcacaca
    19381 tgaacataaa gaccattcta aggctatcac agagcttaca aataatggaa tagatgtata
    19441 ttctagtaaa ggtactttag aagttttagg aatagaaaat catagaacta aagttataga
    19501 aagtgagaaa ttatttaaga taggtaattt taatataatg cctttctcaa ctaaacatga
    19561 tgcagtagag ccacttggat ttttaataaa tcatgaagaa attggtaatc tacttttcat
    19621 cacagatagt tactattgtg aatataactt taagaattta aatcacatca tgattgaatg
    19681 taactatagt aaagatttat tagacaacaa taaaaataag atttatttga gaaatagaat
    19741 agttaaaagt cactttgagt taagtaatgt aattaatttt ttaaaagcta atgatttaag
    19801 taatataaaa acaattactt tactacatct aagtgaagat aacagtgata aggatttatt
    19861 tataaaagag atagaaaaaa atgtaggcat accagtaata gtagctgaga aagggctaga
    19921 aatttattta gactagaggt gataagatgg caaaatatag aattttacaa gctaactttt
    19981 gggatgatgc ttttgtgcta gatttaactc ctgaagaaaa atatttttac aattatttac
    20041 ttacaaatgg tagagcaagt caatgtgggt gttatgaact gccatataaa attatggaaa
    20101 tgcaaacagg atacaacaga gaaactgtag agaagttaat aaaaagattt atagagtatg
    20161 gaaaaataaa atatgattct gagactaaag agatattaat aattaattgg agtaaacata
    20221 atttttctaa aagtccaaaa gtacgtactt gtatcttaaa agaggttgaa aatattaaaa
    20281 ataaagaatt tagagagtat atgtatagag tatgtataga ctatggatac cctatcgata
    20341 cagtatctat agactatagg gaaaaagaaa aacaacaaca aaaagaaaaa gaaaaagaaa
    20401 aacaacaaca aaaagaaaaa gaagaaaaat ctttggtggt tgtggataac ataaaaagat
    20461 attttgattt agaatctaaa gacattgaaa agattgttga tgtatttata catacaggaa
    20521 gaggaattga ctatttagag gaaaaactaa tgttagtcaa aaatacggag aatgtaaaaa
    20581 gcgttacagg atatctcata aaagcattaa aagaagatta taagcttata ccaagcaaga
    20641 acaataaaaa taaatttaga aactttgatg aaacatttac taagtacaca tctgatgaac
    20701 tagacgacat tattaagaaa agtcaaaaag agaagtttgg agtagggagt taatataact
    20761 attaacttcc tagaagttaa aatattggag ggatgaaaga tggagttaaa atttagagaa
    20821 tggaataaaa atggtaaaga aatgtatagt tatgatgaaa tggtgtgcta ttctaaaaat
    20881 ttgcttagag agtgggttta tagtggagtt tatttaccaa caggcaatga aaattctgag
    20941 gttatgatat atacaggttt gaaagattgt attaaaaaag aaatctatga gggtgatatt
    21001 gtttcataca tcttatcatt tgaagaattt ataggagagg taaaatttga agaaggtttc
    21061 tttgtaatag ataatgaagt gctaggagaa tgcgttgggt tatttcatga aattgcagtg
    21121 gttaaagtta ttggcaatat atatgaaaat cctgagatgt tggaaaagat aaggaaacct
    21181 aaagtattgg agggatagaa atgagatttg agataggtaa gacttataag tttgataaag
    21241 agaaatttat agaaattaat ggtgtagaac actataaaaa atataaagaa ctttggattg
    21301 ataacattga aggtgttgaa tttactgtta agaaaatatt tgaaaatggc tatgttcatt
    21361 atccaaatga attctatttt gctttcggta tagttccaga atggtgtgtt gaagttaagg
    21421 aagtaagggg tattagggat gataataatt agaagtcaag atagattaga tttaacgaga
    21481 gttaatagag ttgaaataag caataatcga gtatatgcaa tgtttgaaga tggtattaga
    21541 aaaataggtg aatatgaaag taatgaaaga gctatacaag tgttaaatga gatacaaaag
    21601 tttattgaaa gtggagtgaa aatggattat atagattctt gcagagttag acataatcaa
    21661 gagaaagtat ttgaaatgcc agttaaataa aaagggggaa ctaaatatgg ctaaggtttg
    21721 ggtggatgca ggaacatttt tagaaagaac taaagatata ggagatattg aatgtaatct
    21781 aagagaaatg ataaataaaa ataaacaaaa aaatttaatt ggtagaataa attccaaagg
    21841 gcaaggaaga aaaagtaaga aagtagagtg tactaacatc attactggag agagtaaaat
    21901 atttgtcagt gctgtcgaag caagtaaata tttatatttt acagaacttc atattactcg
    21961 tctagcaaga acaggaaaga ctactaaaaa tggttggaaa gttagatata ttcaagaggt
    22021 gacagatggt attagcaaat gtggagcaag taattaaatt agctgaaaag ataataaata
    22081 agaaaaagtg ttctgttaat aaagctattg atatagctat aaaaatatta agtagatatg
    22141 aatatgaggg gatgattaaa tgagtttgat tgaatacaga ggctatgatt ttgaaaataa
    22201 aaagtggatt tattctaaaa cgataatgtg gagtaatgct gtagactgtt tattaatgct
    22261 gaaagaagat tttaagtggc gaaaagtttg taatgttgga atatgttctg aagaatgggc
    22321 tagaaataat caagaaatct atgaagggga tatattgaaa gaatatgata atttcaatga
    22381 cacaagccaa tatggagttg taaaaaggga tgttggtagt attaaaatat atttggaatg
    22441 gcattatcta aagaagtttg aaggagaatg gatagagatt gtaaataaaa cagaaatata
    22501 tcatagcaga gattacaaag tggtaggaaa tgaatacgag aatttagagg aagttagaaa
    22561 agagctctta gaacgtaagg agaaacttga gaatgaaaat acaaatgcag ttaactaaag
    22621 aaaaggagtt tttcaaagtt tatgtaaatt cagaagaaga agagttggag aaattgtttt
    22681 atgaatttgt atcacaaatg ttagcttgta aaagaaaaaa taagaaagtt caaggagata
    22741 ttgagaaatg aaattaaaag atattataaa acttggagaa aagtattgct attgtcctag
    22801 ttgtggtaat gacaagatag gaaacaatga aggtaaatta atagttgaag aacacacata
    22861 ttatagagaa tgtaaatgtg ggtttaatat attaattgat gataggaagg atgaaatcta
    22921 atgaatatct taactagttt acttttgata gggattagtt ttgtagcagg tagggtttat
    22981 gagtatagat tgaatctgaa agagtgtgaa aattgcgaca ataaaagagg tgtataagaa
    23041 tggatgatag attagagatt tgcaaaaaaa tgtttcctaa cgttactaaa aatactagat
    23101 tactttgtca ttattgcgat ggtagaaata tttgcagtta tgatgaagaa aaagctcgta
    23161 aattattgag agaaaaagag gaggttaaga atgactaatt ttgaaatgat aaaaaatatg
    23221 agtaaaaaag agttggctga atttataaac gaaattggtg ataattgtac ttgtatttac
    23281 tgcagttgtc aatatcgata tgaaatttgt gatgtagatg aaaaatgtgt tgatggatgc
    23341 aaagagtggc ttgacatgga ggtagagcta taaaaatttg gaatgatgaa ataagcaaag
    23401 aaatagaaag attaaaagga tttttatata gggaaaatat ctaattaaaa cagtttagag
    23461 agttgcaaaa tgtattttaa tataaattgt tgttgaagtg ttttgtaact ctcaaaaatg
    23521 aaaataaggg gtgaacaaat gtcaaagtat gtacttagat ggcaaaaagg gttattgttg
    23581 gatgaacgta agataaatta ttcatgtgga agtaaagaaa tgttaaaaca aaaagcagaa
    23641 ttattagcta aagatgacaa gatattactt gtaacaatag ataaagttga agaagttata
    23701 aaagatacta gaagtcagaa aatggctgaa tattttgagg atgaaggggt ggaattatga
    23761 taatacacaa atttataata catgttttgg ataagaatag cgatacacca atactaaatg
    23821 attttgaagg tagagttagt caagatatgg acatattctt tcaaaagaaa atcaacaaag
    23881 tattaagaga taatgacatc agaaaagcag tatttaatga ctatagtagc aatctgatta
    23941 agaaatgttg tgaacaaatt atttatgatg aaagttcatt tttaaataac tctaaagaga
    24001 ttgcagctta tttatttgat gttatgaaat tgaatgctac attagaatct tgcgacttag
    24061 caatatgttt atatactgaa aaagatgaaa agaaagttgc tatattaaaa cttgattaca
    24121 acaagtcgta tactcattct attagctttg aagatgataa atttaatata caaatgtcta
    24181 agaatgaaat taatatacaa gagactaaag caattaaaat aggggcaatt attggtttga
    24241 gtggaatgaa tgacgaatat catctaaaag tattggataa ggatgcagag aaggaagaag
    24301 ctaattctaa gtttgttaca gggtttctaa atgctactaa gataaaagat gataaattta
    24361 agactaagat gtttaaaggc ttggtagatg cttttatagc gaatttatat agtgacatga
    24421 agcagggcga agatgtaaga aatatactgc tttacatgct aagagaaaag caaaaacttg
    24481 atataaatga gtttactgac aaggcaataa agggagattt aaaagatagt tttaaggatc
    24541 atgtagaaga aaaaggtctt gatggaagtt ttaatattga taaaaagtgg gttgagaaaa
    24601 atctaaaaaa tagacatata aaaacagata cagggattga gataaaaggc aaaatagatg
    24661 attttgagga ttttatgaaa tatggcatta gacataatgg gaatggaact gtagatataa
    24721 ttattaaaaa tgtaagtttt tataatgaaa aataaggagg ttacaatgaa agaacaaggt
    24781 cgtttggtac taaataaact tggaacagta gatgaagtaa tgaaaaaagt tgaaacggat
    24841 gaaaaagaaa taaatgttta tttaaaagaa ctaaaagaat gtgaaaaagc aaaagttttg
    24901 attattggta aagatggaac tgaaattcca atagttttca aagatgaaac tatgaacgaa
    24961 tggttaagaa aagaaattga gataagtttt gatgaattta aagatgaaat tgtgcaggtc
    25021 aaacaagtat tacttaaaat tacaggagat aattagaaag ctggaaggtg ctcatatgac
    25081 taacaaagaa atgtgcaagt caaagaatat cacagagaaa gaaagttata agcgatttag
    25141 taaagaaatt tgtaaaagct gtaaaaataa taagagagat tgtgaaagta aagattgtga
    25201 tataacatac aaaaattggt tggaggatat ttactatgta tagtacttat aaagtaaaac
    25261 ttaaaactaa aagaacattg gagcaagtaa gaaatcaaag tgtagatttt gagtattcag
    25321 aagaaggatt aaaaaatacg ctaaaatact ataacttgat tgatagtcta gaagtaattg
    25381 tagttaaatt tggagatgaa tattgtctag ttgattgcga tgaagaagat gaaaaaacaa
    25441 taatggaagc atattatctt ttagaacaag acgaatatac tggatgttat ataaatgaat
    25501 atgagttatt taaaaaagat tgggaaaatg gtagctatta cggggaagtc tgtatggtgt
    25561 tttcggatga tgaaattgag ataattgaaa agttaaggga gggttaaata tgaataaaag
    25621 aatcaaatta aaaaaaagaa tacttcataa aatatgtcaa gaaaactata aggaattaag
    25681 aaaataaaat aaatagtcaa ggtaagtttg tgaatgaaac tagaatgtta tagacttact
    25741 ttgacttata aaagtgagtg tgttaaatgg ctaatatata ttgtgaaaat tataattgcg
    25801 aaaattactt tgaagatatg tgtatgcttg aaagaattga aattaataac ctgaaagtgt
    25861 gtgaaagtta cattgaaggt aaaaatgagc tatataaatt agaaaacgga tatactatac
    25921 atcctaaaga tttgaaaatg gtgaaaagta aagattattc tgttgaagtt actcatattc
    25981 caactggtat tacagtaaaa tgtcgttcta caaatagtat tttaaaaaat aaaaacaagt
    26041 gtttggaagc tctagaagaa gaactaataa aaataaactc acacttagag ctagaagatt
    26101 tattctaaat aggaagtgag cttatgaaac gaagaatatg cagttggtgt ggaaaattat
    26161 tttatcttaa ggaaaaatct aaggatattt attgttgtaa ggactgtaga aagaaagcta
    26221 agaaggtgaa aaatgaaagt taattttaca atagatggag aaccaatcgg aaaagaaaga
    26281 cctagattta atttgtctac taaaagaacc tataccccta ataagactag agattatgag
    26341 gacctaataa aatggctata tcaatctaaa gttaaacatt actttgaagg ttatataaaa
    26401 atgactttaa gatgttacta ttctatagct aaaagtaaca gtaaaaaggt taaagagcag
    26461 aaaaaaaata atgtgttaag acccagtaag aaacctgata ttgacaatgt tgttaagatt
    26521 gttgctgatg cactcaatga gatagcttat aaggatgata cacagatagt tgaggttgta
    26581 gctagtaagt attatagtga tagaccaagg attgaagtta tattggaaga tgttatttaa
    26641 ccaacggaaa aattcgttcg ttagattagg tgctcagttt ttcataaaaa tgtgaaaaag
    26701 ctaaataaag aatatatcaa atgataaagg agagatagac tatgaatgaa aacataaata
    26761 aagaaataac agtacttgga actttagaag ttgagggaat gaaatttcat gatattgagg
    26821 gtgggtttgg aaaaggcaag aaagcaatgc tagtaaagga tatagccgag atacataata
    26881 gagaacttaa acatataaat gagctaatta ataataatat aaagagattt aaaacaaata
    26941 tagatattat agatttaaaa gtgagtcgtt ctgaacgact aggtgattta aatgctaatc
    27001 cttttgaagg attaggttac gaggctgttg ggtatagtaa acaatctttt aatcagtcaa
    27061 gaaatgttta cttgttatct gaaagaggtt attcaaaact acttaaaata ttggaggatg
    27121 atgtagcttg ggaacaatat gaaaaaatag ttgatggata tttctctatg agaaaagaat
    27181 taaataatcc tcttttaagt gcatcaaaag agttacaggc tatatttatg ctagataaga
    27241 aacaagaagt cttagagact aaaatagaga atgttaatga gaaattagag aattttatag
    27301 atgatgctcc attatttaat atagagtgtg agtgtattgt taaagaagtt aagagagtag
    27361 caacaaaatc acttggtgga catggaagta aggcttataa aaataaatct ttaagaggta
    27421 aagtatacag tgatatatat catcagatta aacgagaatt tggagtagat agttataagg
    27481 ctataaagcg ttgtcaatta aataaagtat tagagattgt aaacagttat aagttaccta
    27541 tagtgtttga ggaagaaata agacttttga atagtcaatt atcaatagta agttaagaaa
    27601 aaagaagaag gagtgctccc acactccact tgtcaaaaat ataaaacttt tatatacaaa
    27661 tattattata acataaataa ttgataggag tgtgtgagta tgtctaaaac taaaaaagag
    27721 ttttttaatg caactaagaa acaactttct aattacaaac aattaaatgc aaatataata
    27781 aaattaaaaa atgaaataca aatgttgaaa gataattcag ttggagattt aacaaaagct
    27841 ataagttatg atagtgtcaa aacaggaaaa acaaacaaaa ctagcaatat gattgaggat
    27901 gctattgtta atgtatcaga cttaataaca gaaaaagaaa tagagttata tgaagcagaa
    27961 ataattaaat ctacaataga tttagctata aggaatttaa aacctataca cagacaaatt
    28021 attgaactta aatatataga tggtctaatg tggcaagaaa tggttgatat aatacattta
    28081 gaagaaagac aattaagtgt aagagctagt caagctatta gctcaatatc aatagcactg
    28141 tttgggaaga aagcattaat agagcaagaa ccactttttg aattgttaga ttacaaacta
    28201 aattaaaaag taagagtaat tttgagtgct gaaaatgtgc aggttttttt gttttagaca
    28261 tgagataata gtattgtgga aataaagatt tccctctcaa aacttaataa ttggctaggg
    28321 tataagggat tgccctagcc actattcaat aatagagaaa gtaataatat atttgcgaat
    28381 atgttataga taagaaagga tgccaaaatt gcacgctcta ttattataaa gggcttagat
    28441 gtcagcgaca gaaagtcttt tatgtgaaca gactaggcag agcgtgagga cgctgttagt
    28501 tcaattctaa ctatgttcaa atattaatct aacggataca ctaaaaagta gaaaaattga
    28561 gggcaaaatt ttatattttg tatcttaatt cagaagtcta aaaaatcggg tggggcttgg
    28621 taacctcact caccatgcaa gtactggttt agtctaggtt cgattcctag aacttgcgac
    28681 ataatatgta tctccctatt aaaaagacta agtttgggta agcttagtct tttaattttt
    28741 aaaaagaaaa aagaaatttt tattgtcata atactatttg tttaggtata ttataatgtg
    28801 catacttaaa attaaatact tagcaagtat ttgaattaat atacataaaa tatatgacat
    28861 aattttttta tagtgtaagt tatttaaatt aatacaaata acagtaattt tattatataa
    28921 aatgtacata ttgtgaataa taataataaa atcatgtaca aaatgccaac tgataattcc
    28981 tcgaaatgta ttgcacattt gacgtaacgt cagtataatt aaattataat agtgaagtgg
    29041 aaggtggtac ttatggctac aaaaagtatt ttaaaaaatg tagatgtaag aaaaaaggca
    29101 tttggaagaa atctagtatc agctctagaa aatgctaaaa ataaacaaga aaaagaagtt
    29161 gtattaagta aaaaatgttc agaagtacca aaagataaaa taaaggatat attcaggaga
    29221 ttttaatgag tggctattca attgtgaact taagtaatat gctagaagag ctggaggaag
    29281 aagaagttaa aaagattctc tccagttttt cttgtcctct taataaagat gtagaagaat
    29341 ttttaaaaaa caaagctatt gaattttcta aacagggttt agctagtaca catttagtat
    29401 taacttctta taaaggcaag cctgttataa ttgggtattt ttctttagct aataagtatt
    29461 ttacgataaa aagaaaaaca ttatcaaact ctttagctag gaaaatagtg aagtttggac
    29521 aatacaatga agaactaaga agatatatta ttggagcacc tttgataggg caaataggaa
    29581 agaattattc aaataattat aataaattaa tcaagggtga tgaacttcta aaaattgcat
    29641 gtgacaagat aaaagcagta cagttagata tgggtgggaa aatagtgtac cttgaatgtg
    29701 aagataaacc taaattaatt gaattttata aggataatgg atttgtagat tttggtaaaa
    29761 gaagtcttga taaagatgaa acagattcat tggatgggga ctatctagtt caaatgttaa
    29821 aatatctaaa aaaataaaag tacataaaat ctaaaatgac tatctttact ttatagatgg
    29881 tcttttttta tacaataaat taaaaggaga aaagaaaatg aaaaaaataa atgataattt
    29941 aggttttgga attgcttttg aggaaataaa gagtagtagc aaagaactag gtatgagatt
    30001 accacaatgg agcgaggatg ttgttataaa gatacaaaga ccagacgaac atagcaaaat
    30061 gacagcatct tatttatatg tggaaagtag atatggtaga gttccttgga aagaaacaat
    30121 gatagaacta tttaatgaaa aatgggaagt agtagaaata taaaatatat tataagttat
    30181 gggaagtaat aagataaaag gagaataaat tatgaatgat tataaagaaa aaactgaacc
    30241 aaaattaaat attttagtcc agtctaaagt tgatatttta tgttctaata gctctactat
    30301 gaatggagta gacacttcag tttatataaa actttcttta gataatagag ttattggtaa
    30361 cttaaccaat gaagacgtga aatatgaaat aatagcatgc tttaatgaat gtttagagga
    30421 gattaataga aaaattggaa gtgaagaagt aatcgataaa ttgcaataat ctttattccc
    30481 aaaacgacaa acaaacgagg tggtgatgtg caagatgtca aagaaaaggt aaaacaagat
    30541 tacctaaaag gtatgaaaca aaaggaaata tcagcaaagt atgacattag tttaaatact
    30601 ttaaagtcat ggattaaaag atacaactgg gctagtgaaa aaaagaaggg tgcacctaaa
    30661 aataaaaggg gtgcacccat aggtaataag aatgccactg gacctcctgg taataagaac
    30721 gctgaaaagt ttggtttctt ctcaaaatat ctacccgaag agactcaaga cctaattaat
    30781 gagataaaga ataaagataa attcgatatt ctttgggagc agataacaat tcaatatgca
    30841 gcaataataa gagcacaaaa gataatgtat gttaaagata aagaagaaat gattaaggaa
    30901 cttaagaaac atgaaagtac agaaaatggc gagaagatag agtatgaatt tcaatttgca
    30961 tgggataggc aagcatcttt tcttaatgca cagagtaggg caatgagtga gttaaggagt
    31021 cttataaagc agtatgatga aatgattcat aaggattgga atttggctac agaggagcaa
    31081 aaaactagag ttgagaagtt gaaatgtgag gttgctaacc taaataaaga tgatacagat
    31141 aaagagataa ctgtaagagt tatgaaagcg agtggagaaa atggaaagta aatttatatc
    31201 aaatgaccac ttttatgatt ttatattcaa ttgggattac aaattttatt ttcttgttgg
    31261 aggatatgga agttcaaaga gttatcatgt agcaactaaa ttaattatta agcttcttga
    31321 agaaaaaaga aaagctttag tggtaagaga agtatatgac acaatgagag atagttgttt
    31381 ttctctatta gaagaagttg cagaaagtat gggtttagga gatatattat catttaaaac
    31441 aagtcctatg agagttatat ttccaaacgg aagtaaaata atatttaaag ggatggataa
    31501 tccagctaaa ttaaagtcaa taaatggtgt atctataata tggttggaag aatgttcaga
    31561 ggttaaatac actggtttta aagaactctt aggacgtttg agacatccta gtttatctaa
    31621 tcatataatt tgttcaacta atccaattgg cgaagataac tggacctata aacatttctt
    31681 tagagatgaa gaaaaagaca ggtttgtatt agatgatact gtgttatata aaaatagaat
    31741 agtaataaaa aataatacat attatcatca ttcaattgca gatgataatt tttttctgcc
    31801 cgaaagttat atagagcaac ttgatgaaat gaaagaatat gactatgact tatacagaat
    31861 cgcaagaaaa ggaagatttg gtataaatgg aactaaagta ttaccacaat ttgaagttat
    31921 ggagcatcaa gaagttataa ataaaatatc taacatacca caaaggtatt ataaagttgg
    31981 attcgatttt ggatttgaaa aatcatacaa tgcactttta agattagcta tagaccatga
    32041 aaacaaatat ttgtatatct attgggagta ctataagaat caaactacag atgatgtgac
    32101 tgttaaagat attaaagagt ttaaagatac tcaagaacta ataaaagctg atgctgctga
    32161 acctaagact ataaaatatt ttagacaaca aggttttaat atatctggtg ctaagaaata
    32221 cgctggaagt agactacaaa atactaagaa ggttaagaga tttaaaaaga tttattgttc
    32281 aagtgagtgt aaacatacaa taaaagagtt aaagaaccta acatacgcta cagataaaaa
    32341 tggaaactta atatatgatg aatttaatat agaccctcat acatttagtg ctatatggta
    32401 tgcacttgat ggttatgaag tgtcagatat taaggagtta aaatataata atgatattta
    32461 caataaaggc ttaggcttga agaaaaataa tataccaaat caatataata agaagggagg
    32521 ggctgtattt tagtgagtat aaaaaatatc ttgcttaatt tggatgaaaa agaattaaaa
    32581 gatagaaaac atgcagaaag agatttcttg ttttatttag gtgaatgtag aaataaaaca
    32641 atgggattat tagatgatga ttttctaggt caaagttgga ttacttttga taatttagac
    32701 tatacacctt ctcagattat agataataag gtaaaaccac ttataaataa acaagctcgt
    32761 tttatgtttg gaaaagaacc aactataata cttaaagcat atgagaaaga agataaagaa
    32821 acatgtgagg aattaagaca atatattgat tcaatattaa atgccagtaa gttttggagc
    32881 aacacactaa aagcttttaa gatagcaact ataacaaaaa gagttttatt aagattagaa
    32941 gctgaaccaa atcaacctat aaggctattt tatcattcta tagatgattt taaatatcaa
    33001 gttgatagta atgacattac aaaattaaaa tctgttatat ttgttagatt tgatgcttca
    33061 accataaaag aagtaacagc gaaccaaata tggtatagat atacttatta catgaaagaa
    33121 agtagtgtta ataaccaaga aagttgtttt ttaagagtag aaaaatttaa aggtgataat
    33181 ttatctaagc ctattgaagt aaaagaaagt gataccaagc tttctaaaat accatgttgg
    33241 ataattgtta atgaacaaag tattacaaat atcacaggca ttagtgatat tgaggattta
    33301 aagccattac aagatactta taataaaaga ttatcagatt ttaatgactc tttaaaattt
    33361 ctaatgtttg ggcaaactgt tgtagtagat gcgacagaag aaacagttaa tgcctgtaag
    33421 attgctccga atgctttaat ggcattaaaa acacttgaag aaggttcaga aaaaggaaaa
    33481 caagcacaag catatagagt tgagagtagt ttttcaaatg cagacccagt cgactccttt
    33541 tttaaaagac ttgaggatag tatgtacgaa aaacttgcaa tccctagacc agagcaatta
    33601 cagaatatac ctagtgccaa agctttgaaa tatttatata cggaacttat tgcaaggtgt
    33661 tcggaaaagt ggaatgattg ggaacctgca ataagaagta tgttaagact aatcgttgaa
    33721 gcatgtagta aatttaattg ttatgatgat tggaatcatg attgggatga tttaatgttt
    33781 tctattgtat taaataaaaa ttatcctatt ccagaagatg aagaagattc gaagagattg
    33841 gcacttgaag aagtgaacaa taatgttaga agtcatagaa attatataaa agaatttggt
    33901 gatgatgaag attatgagga agcatttaat gaagtattgg aagataatga aaagatacaa
    33961 tcagtagagc aagaccaatt cagaaaagac gcagaaatag aaattgatga tattgatgaa
    34021 gaattaaata gtaaatctaa taatagtgat gagtaatgag gtgttgttat gaaggacaat
    34081 acttatacaa agaaagttct tgaagctaga aaaaaacttt tattattaga taaaaaaata
    34141 caattagaga tattaaatgt atataaagat gccagtaaaa tcattttaaa tgacattgct
    34201 aagaataaag aattaaattt aagtaccaaa tacctaaaaa agctaaataa atcaatcgag
    34261 aggtatatta atgagttaaa ccaaagatta gtacctgttg cagaaaaaag tgtaatagaa
    34321 gcttctaata tagctaaaga attacaaatg tattactatc aatcaatagt tccaaataca
    34381 gctatgaacc ttgcatgtga tgctatgtgt ataaagacaa ctactggcgt ggtggaaaaa
    34441 ctagttacag gtaactttta taaagataaa aggtcattag atattaggct ttgggaatat
    34501 agtagtaaaa atagaaaaga cattgataga ttaataaaag ctaatgttgc tagaggtgct
    34561 aatgcaaaaa ctttagcaaa gagtttagat aattatgtta atccagttag aaaaacagag
    34621 gctaaaacat tagaagttgg aatgaataaa agtatatctt atcaagctca aagacttgct
    34681 agaacctcta taacacatgc ttttgtagaa acaagtgttc aaaatgcgat aaataatcct
    34741 ttctgcgcgg ggttacaatg gaacctaagt cctcaacatt atattagaca agtaaagtgg
    34801 aggggcgagg atgaatgtga cgagtatgca gaacaaaatc gttttggact aggtgaggga
    34861 gttttcccac ccgaaaagta tcctattcca caccctaatt gtttatgtta tccaattcaa
    34921 gtgattgtac caattagtga agcttctaaa atgatggaga actggatagg tggaggagat
    34981 aatgatactc ttgatagttg gtttgatgga ataagacatc aaaataatag tgttccaatc
    35041 attaaaaaac aaagaaataa aaagaataaa atagctacaa atagtaaaga tgatagaatt
    35101 aaaaagaacc ttactaattc aattaagaat aagtcaaaga aagcacctaa acaagttcaa
    35161 aaatatatta acaagtatgt gcaaccaagc aaaatactaa ttgataatag tcaaaaatac
    35221 cttttattta ttatactaag gttgatttaa ttggtattaa tccaaatatt aaagaattta
    35281 aatcatataa taaagaagct gctttacttc atgagtttgc gcatagaatt gatataaaag
    35341 aaattaagag ctataataat attaagttcc aaaaagctat tgaaagtagc tctatatatg
    35401 ttatgaagaa tattgaaaac ttgcaaagta tatatactaa ttctaatgag ttgtacaata
    35461 atgaatttat tagtgatata ctaggagcat tatctaataa tgaatttgag gatttattag
    35521 caactcatag cgaaaaatat tggagtaaaa acagaaataa agagaaagaa atatttgcta
    35581 atttatttac actaaagtat caaaataata aagaaataaa tagttttatt aaagaacatt
    35641 taaatgattt ggataaaata tttaatgaat tattaggagg gatttagatt gcttgaagaa
    35701 atgagaaaag ataaaaagct gttagagttg agacgattat ataaagaaaa atatggtaaa
    35761 aatgcacctg gtttcaacta tgatgaatac aacagttatg ctgagtacaa agaaaagcta
    35821 aaagaattga tacaaaagta aagtaagcac ttattaatta aaggttaatg agtgctttta
    35881 ttatgcttaa ttttaaggag gaaattgaat gttagagtat tttaaaaagt tacttggaga
    35941 tgaagaagga caaaaagttt atgaaaaatt atctaaagat aaagaaaata agcttctttt
    36001 agataacgtt aaaagcccta aatacgttga aaagacagaa ctagaaaatg ctaacaaaga
    36061 gattaaagag tataaaaagc aaatagggga tagagataag caattaaatg acttgcaagg
    36121 taaagttaaa gataacaaag agctatcaga tgaaattgaa agtcttaaaa atgcaaataa
    36181 agaaattaaa gaaaatgcag aaaaagaaat tgaggtttta aaattcaata cagctcttga
    36241 aagggctatt gaaagttata atcctaaaaa tgcaaaagct ttatcagcat taatagataa
    36301 ggaaaatatc aactttgtag atggcaaaat tattggatta gatgaacaga taaaagctta
    36361 tcaacaaagt gattcttatt tatttaataa tgaaaaaaat aaaggagatg atgaaaatgt
    36421 aggaactgga ggactagaag gaggaacaac atcattactt gataatgata acgaaataag
    36481 tagtataggt gagttgctag ctgactctaa agtaaaaagt gaaaatatag aagctcaaaa
    36541 gaaattcttt ggagaagaat aggaggaaaa tatatgagta tagaaaaatc tgagatttac
    36601 atgggggaaa ataaaaccat attaaaattt gcaggaaatt tatttcaaaa tataaatatt
    36661 aaagtgaaaa aaactgatgt agctgtagta gatggaaaaa gaattttaaa agcaggtaca
    36721 ttaatatcaa aagatggaaa attagtagat ggtacaactg ttacaaatga taaagctttt
    36781 ggcctagtat atagaaatat agattttact cattcaaatg gtaatgaaag tattccagta
    36841 ctaatatttg gatttgtgga tgaaaagacc ttaccatcag ttataccaga agaagcaaaa
    36901 caagcaatga aaatgattat gtttttataa aataaatatt aggaggaatt aaaatggatt
    36961 ggaaagactt tatagactct aaggagatag ctaaatatat aaaaaagtta ccactagaaa
    37021 tgctaatagg cgaatctttg tttcctagaa aaaaacaaat aggtatggac ctaaaatata
    37081 ttaagggagc gaagaaaaaa ccagtggtgt taaaacaaag tacttttgat gttgctgtaa
    37141 aaataagagc attaaaagct caaatagaag ttaaatcaaa gagaatgcca ttttttaaag
    37201 agagtgttct tgtaaatgaa gaagatagac aacagttact tttagcttct aaagctcaaa
    37261 acaaagaact attattaatg ataatttcac aaatatatga taattattta gcccttgtag
    37321 atggtggcga tatgcaaatg gaaagaatga gaatgcaagc tctagctgat ggagtaataa
    37381 atatagtatc agaagatgga gatttagtat ttgactttga agttccaagt aatcataaag
    37441 aagtgttaac tggaagtgca acatgggata atccagatgc agatattata ggagatattc
    37501 aaagatggat gagaataatg agagatgaag ggaagccgtt acctaagcga atggtaatga
    37561 caagtaaaac ctttgggtat tttgctaaaa acaaagctat taaattggat atagataaag
    37621 atggtagagt tattttaact gatgaaatga ttaaaaatta tcttaaaaat aaagttggtt
    37681 tatctgttgc catagtaagt ggaacatata aattagaaga tgaaagtgag gaatcttatt
    37741 tccctgacaa caaaataact tttattccag acggagattt aggaaaaact tattatggca
    37801 caacaccaga agaagctgac aaagtatatg gttctaaatt agattgttct gttgttagaa
    37861 ctggtattgc tataacaaca atgagattaa ttgacccagt aacagttcaa actaaggtat
    37921 cacaattagg tatgccaagc tttgaacgtg ctgatgaatg tttcttcgct acagtagcat
    37981 cttaaaaaag gaggatatta tggctaaaaa gaaagagaat ttaatgcaag taaaagcttt
    38041 agtttactta aaatatgata atgactgtta caaaatagat gatatatttg agattagaaa
    38101 agctgaccaa gaacttatgg aggaaaaagg atatatagaa gtaataggag aagctgagga
    38161 aaagaataat aatgagattc tcaacaaaga tggtgagtag acatggctat taacaattta
    38221 gataaattaa aactcaattt gcaagaagaa gagtatcctt attttacaga tgaacaactt
    38281 gtgatgttat tagaatctaa tgagaataat gttttaaaag cttcatggag aggttgttta
    38341 ctcaaaggtg ctacagatga ttgtattaaa ataggtccta tagaaactaa aagtagtaat
    38401 agttcatact ggttgtcatt agcagatata tataaaactg attatttaga agaaaaatca
    38461 aagaatgaaa caactaacac aggatataaa acatctatga caagagttga tggtcaatga
    38521 gaaaattaag agctgataaa ataataaaaa ctattaatcg aggtatagct ttaaaccctc
    38581 aaataataac tatagagcag gaaattaaaa aaatagtgga tggagctatt gaggttacaa
    38641 atgaagtaaa agaactaaca gtcgttatat atcccgaaaa aagcaacgat acagtagtaa
    38701 gtagtgaaac tattggtaca gtttataaaa ataaaaattt tggtatggtt gcagataaag
    38761 aagctgattt aagattgaat acagaaaatg agataacttt taaatgtatt gaaggtacta
    38821 tgaaattaaa ttatgtaaat cctattgtag ttgaggaaaa gatttgtgga tatatatgtg
    38881 gtcttgaaaa gatagattag aggtgggtta gtgtgagtgt gtttactaaa gctataaatg
    38941 aaattgatag aaaaaaggct acaatgccaa ttttgtgtac gaatatagct tttatgttag
    39001 aaggagaagc taagaataat gcaaaatgga ctgataggac aggaaatgca agacaaggca
    39061 taacaggaac aagtttaggt ggaggaaatc aatacattgt taggttaggt catggcgttg
    39121 actatggaac tgttttagag gaaggttcag cacctcatgt tataaaacca aaaagtgcaa
    39181 aagctttgtt ttggaatgga gcttcacatc ctgtaaaaca agtccaacat cctggcacta
    39241 aagggacaca ttctttagag tctacagtca gtaggaatat atctaaaata ggtaaattag
    39301 tagaaggaca ttggagtaaa taatatgaga gcaggaataa gaaaagcctt aattgataac
    39361 ataccaagat taaaagattg ctatgaacca actgttccta ataaaaaaac tataaaacct
    39421 tatattgtag ttgtccaagg tgaagatgct gacaatgaag gtgatgttat aggttttaga
    39481 aggaccataa atatttggtt atatgagaaa aggactacat ttaataaact ggatgaactt
    39541 acaaaagaag tcatagagac tttagatttt aaaactataa cagatgatac ttctaatgag
    39601 gtatttactt gtatttatga gggtgcagtt ggtcaagatg ttatagatga ggaatgggaa
    39661 gccataataa gatgtctaag gtttagtgta atagctttag atgataaaga agatataact
    39721 aatgatagat gggttgaagc tttatctaag tacacaaaaa atttattaga aatcgagagt
    39781 tacaaggata attggaagaa aaattttata gctccatgtg cattatggcg aactacaaat
    39841 attgaaaaca aaaggattaa ctatcatctg attgagatta ctaaaactat gaaatgtcat
    39901 gttgttagca aaaataagga tgaaatagtt aagcttcttg aagaattaga gactaagcta
    39961 ataatagata agagagtaag acttagagaa gataaaaata tgtatttaac tcttgtaagt
    40021 gtagttgaag atagagaatc agacatgttt tctaatggtc aattaactgt agtattcaaa
    40081 atgataggta agataaaaag agaagggcct attatgaata agatttatag taatggaaat
    40141 ttaaaatagg aggtgcaagg attggctgaa gcaattaata aaaagactaa tataagtaag
    40201 caggaagaaa aatattcaaa agaagatttc ttaaaaaata gtgaagcact tggctacaag
    40261 aaagaagtag ttgcaggtgc tttatttaat tgtaagaaag atgaacttac aaaagtagag
    40321 ttcgagaagc ttataaaaga tttcttagaa agagaggtga agtaaaatgg caactggcgc
    40381 atggaatgaa aaagagaaaa aagatatacc tggtttttat aacaggttca agactcaagc
    40441 agaaaagtct acaaatacag gtttaaaggg tagattagca atgcctatta gggctaattg
    40501 gggagatgta ggtaaggtaa taacaataaa aaatgatttg agacaactta aaactttatt
    40561 tggtgatgat atgaactatt cagcatataa gttaggtaaa ttagctttat tagggaatgt
    40621 aaaggaatta cttttatata ggcttgtaga tggaaatcaa aagaagggca cattaacact
    40681 aaaagatact acagaaaata ctgcaaaaga tgtaattaag ctagaaacta agtatccaac
    40741 tagcagaaat tttaatgtaa caataaaatc caatttagtg gattcagata aaaaggattt
    40801 tattttcttt gaaggaacta agcaattatt tagttcaagt gtcaaaggta ctatagatga
    40861 aatagtgcta gaaataaact ctaatttaga taatgagtat gtaattgcaa ctaaagtagc
    40921 tgatagtgat acaacattag caaacttagt aaatgtagca cttgagggtg gaaatgatgg
    40981 ttgtacatct ataacaaatg agtcttatct aaaagcatta gaagaatttg agagatatag
    41041 ttttgattct tttgtacttg atggtgtagc tgaggaagct ttgcaggaaa ctacaaaagc
    41101 ttgggtagct aaaaataaag aattaggaaa aggcatacta ctttttctag gtgggaaaaa
    41161 agaggataat ataaaacaga ttaatgataa atcaaaaagt tttaatgatg aaaatatagc
    41221 taacgttggg agttcagctt attatgaaaa tgtaaaatat acacctagcg aagtagctgt
    41281 ttatatagga gcattagcag taagcaaagg cataacaggt agtatatgta atgctaagac
    41341 tatatttgaa gaagtagaac cacgattaag tcaatcagaa gttaaagagt gtttaaagag
    41401 tggtacttta gttttagatt ttgatgatgg agacgtaata gttgttgatg atgtaaatac
    41461 atttaaaaag tatgtagatg ataagaatga agctatggga tatatctcta atattatgtt
    41521 tattaacact ataaataaag atacttcatt gaaaagaaaa gagtttgtag ggaaaatatt
    41581 taatgatgcg acagggcaga caactgttat atgtgcattg aagaaatatt ttgaagaatt
    41641 aatgagtcaa ggtattatat ctgaatttaa tgttgatata gatacagagc ttcaagcaac
    41701 tgccaaagcg gatgaatttt attggaaatg ggatgctatt aaggttgatg tgatgaaaaa
    41761 aatatatggt actggatacc taggataaag gaggttatag attatgtata atgatgatta
    41821 tatagaagaa gccagttttc tgaatggttc cgatgtagtt atacttattg atggtgtaga
    41881 agagctatac atggaagaaa taaaagctga ttttgagcaa gatgagcaaa gtatcaaact
    41941 gttaggttgt caaaatgaaa tatcaagagt tgggactact aagggttcat tctctttaag
    42001 cggatataaa gtaagctcaa aattcgcaaa gttaggtttt aaatcttttg aaataatata
    42061 taaattaaca aacccacaaa cattgggata tgaaagtatt agattaaaaa attgtagatt
    42121 aaagaaaata ccacttataa attcaaaagc tggtgaagat gtaaagatag agctagaagg
    42181 aagttataga ggatatgatt tattaaacga actttaaaac aaaacagtgc agcactcaaa
    42241 gctatgaaca attaattttg tttgtagctt tttaaaatta aaaatattgg aggaataata
    42301 tgtcagagat atataaaaga gagctagaaa atgaagtaga agataatgat ttagatgaag
    42361 aaatagaaga aacaaatgaa gatagattga agatgaaaga ggatgaaata atagcaaagc
    42421 tattggagga ttcaccagtg cctgagagaa ctgtgttttt agatagatta ggaataccaa
    42481 ttactttgaa agcattaaca gaaaaagaga ttagtaaaat aagaaaagat tgcactaaaa
    42541 ttgtaaaggt ccaagggcga agagaagaaa aattaaatga tgatgagttt actctagcat
    42601 taatagaaaa aggaactgta aaacctaatt tttctaatca aaaattactt gatgctatga
    42661 aagtaacaaa tgctagagaa tttataaaga gaaaattctt agctggagaa ttaagcaaaa
    42721 taagcgacca aatattggaa ttatctggct tttatgatga gataagtgat gatgatataa
    42781 aaaactaata aaaatggggg gacgactgac tgttttaaat aacatctttg ttaagcatca
    42841 tgttccccca gatatttttg ttaagaaaaa tttgatgtct caacgcttaa tgaaagtatt
    42901 tactcaaaat gaaatagagc aagagaataa agctatgaaa aaataaatat ctgtagaaag
    42961 gtaggtgagg gaaatagcta aaaaggaaat gtatcatatt gatgttgtca taagtgcaaa
    43021 aggcgacgga gaaacaaaaa gcaaactaag tactatggaa aaatacatga agcagacaga
    43081 aaaaaggatg cagacactta atagaataaa agttaatcct gctataaaag ctacagataa
    43141 agcttcaagt gttgtaaata gagttaataa caatatgaat aaagcaaaaa aaactgttac
    43201 agcaagaata aaggctacag ataacgcgag tccagtagcc aatagggcta gcaataatgt
    43261 taataaagct aaaaagacag taacagcaag gttgaaagct acagataatg cgagttctac
    43321 agttaataaa gttaataata agataaaaga agttgctaaa cctgtaccac ctgtaataat
    43381 tagaggacaa gatgagtcta gttctataat agataaagtg aaagctaaga ttcagaactt
    43441 aaaagctgat actattataa aaataaaatc tcaagctgat gaagctataa acactatttc
    43501 tcgaactaaa aataaattgc aagaatttgt gagtaagaga tatgaagcag cagttaagat
    43561 tagagatgaa gctagttcag cacttggagg tcttacagga aaaatagatt cttttgtaaa
    43621 tggagctatt agtaaatttg ctagactggc tactaccgca ggagctttaa taggtggaat
    43681 tggtgtaggt tctgctgtaa aaggatttgc aacttttgaa caaagtatga agaatacaca
    43741 agccgtaagt ggagcaacag gaaaagaaat ggaagcttta actgcaaaag ctagacaact
    43801 tgggagagaa actagtttta cagctaaaga tgcaggagac gcaatgtatt atatgggtat
    43861 ggcaggatgg aagtccgagc aaatgataaa agcaattcct gacgttctta acttggcagc
    43921 agcaggagga acagacttag cactaacgag tgacattgtg actgatggac taacagcgtt
    43981 aggaatgact gcaaatgaca caactgaatt tgttgatgtg atggcagcaa ctataacaaa
    44041 ttcaaataca agtgttgagt taatgggtga aacatttaaa tacgtaggct ctatgggcgg
    44101 agcattggga gtatctatga aagatttatc tcttgcgaca ggtctgatgg ctagtgcaag
    44161 tgtcaaggga agcatggcag gtacttcgtt aagaggaggt ctagttagat taataaagcc
    44221 accagaagaa gctgcttctg caatcaaaaa atatggaata gaattaaaga aaaataaaaa
    44281 tggaagctta gatttagcag gaacaatagg aagtcttaga gaaaaattag gaggattaga
    44341 agatgttgaa aaaggtgttg caatatcttc tatttttgga agaacagcca tggcaggttg
    44401 ggcggctgta gtaaatgcta gtgaaagtga ttttaataag ttaactacag ctattgcaga
    44461 atcagaaggt gaagctaaga gaattgctga tatgaaacta gataccctgt caggacaatt
    44521 tgaaatttta aaaagtgcta ttgatgatgt aagaatatcg gtaggaagta aactaggtcc
    44581 tatgactcgt caatttgtag aaaaacttat aaaagatatg cctaaaatag gtgatgctat
    44641 agttggagta gtagaaaagt ttgttaataa tttcgataaa ataaaagcag gatttcaagt
    44701 attattgcct gcaattggtt ccgttatagc ctctgttatg gtacttaaag ccacctttgc
    44761 ttttggagga gctattaaaa gtttaagttt acttgcaaac acttttggta cagctaaatt
    44821 agctgcattc ggattaacag ttggaatagg ggctattgtt atagcttttg caggaatgac
    44881 tgttgctatt tctaataata aaactgctat gatggactta caaacacgtt ttggttcgtt
    44941 tggtgaatat gtaactacaa taatggaaac tgtaggtgga gttataaagc ttacgctggg
    45001 gaatctactt ataatgcttg gagggatagg caaaggaata ggtattcttt tatcagataa
    45061 gagctgggat gaaaaagctt cttcattaaa aaatcttttt ggtaaaacta cagcagagat
    45121 taaaacaaat acaaaagaag ctttgagtga tataaatgga gaaacttcta atgcaacagc
    45181 tctattaaaa aagtctactt caaaagagtt acaaggtgtt actaaagcgt tctcaactgc
    45241 ttttgaccaa tctaaaaatg taacagagaa gaaatctaat gatatagcta gagcattaac
    45301 gaatggatta aaaggtttag atgaccaatc tcttactatg atgcgaggat taaatgataa
    45361 tatggcgata attttatctg gtgtgacagc agatatgaaa cctagtgaca aagttaacaa
    45421 aatcaccaaa aatcttgatg atgctttcaa ggcaggtaaa ttaagtgctc aagaatacag
    45481 aagtagtata caagaaactt taagttttat aagtaaatat agcgcagatt cttcaaataa
    45541 tttaaaacaa ggcatgagtg atgctttcaa tgccttcaag gaagggacaa atgtaagtgg
    45601 attaaaagac ggagtaacag gaatgttgaa ctctttaaaa gctacagggc cacaagcttt
    45661 agaaacctta aaaggtctag gaggtaaagc aagcgaaatt tttaagggag tggattttaa
    45721 ttcttctata gatgcacaga aaactaaagt attacagaat ctaaacaatc taggtttgga
    45781 aggaacacaa gctatagata cattaagaac tattttctca caagcctcta ctgcactaga
    45841 tactacaaat ctaaaacaag gtttaagcga cacatttaat tcattcaaaa ttggatttaa
    45901 tcaagatgga ataaacggag cgataagtaa catgcttgga actataaacc aaataggacc
    45961 acaaatgcaa cagacattag gcaaaatgga tggggaaatg gggcaaatat ttgctaatgt
    46021 agactttagc actccaatag aaacacaagc ttctaaagtt ttagaaaacc taaataatct
    46081 aggaatagaa ggaccaaaag ctttagaaac tgtaagaagt atatttgctc aagcttcaag
    46141 tcaaatacaa ggttcagctt ctcaaactgc tcaacaagca aatcaacagg ttgcaaatgc
    46201 cctaacacaa gaaaacccag ctgtgcaaca agcagggcaa caattaggta cagatttgac
    46261 aaatggtgta gtgaatggag ttcaaacagg ggtgccaact gttcagcaaa aagggaatga
    46321 attaggagta gcaactcaac aaggtgttac agatgcttta aataatgcta cacctcaaat
    46381 taataataac aatcttacaa ctggtataga tacagcattc aaccaagcta ctgcaacagt
    46441 tcagcaagga gcgacgaata tgtacaatgg agcaaagcaa agcttcagtc aattagccca
    46501 agttggaaga gaagcaggaa gtagtttata taacggtgca acaacttctt ttaatatgtt
    46561 agcatcaaat gtaagggttg catgtagtag catgtacaac ggagctagaa catcttttac
    46621 gagtttaagc agttcagcga taagtgctat ttctgctatg tgtagttctg tagtaagtca
    46681 agttagtgct atgtcgagcc aaatcattag ttactggaat agtgtaagag caaccgtttc
    46741 agcaccaatc tctgcatctt ttaatgttaa gactactcaa acaactgtca aaagaacagt
    46801 aaatgaagga ggcgggatac taagtaatat atttggtcga tttgctgatg gtggagttgc
    46861 aagtaaacca agtatttgtg gagaagatgg agctgaaatg gtgattcctc tttctaacaa
    46921 tagaagaaat cgagcgatag gcttgtatga acaaacaggc aaaatgttag gtttaagctc
    46981 aaccccatca caaaaaattg gagaaagtta ttctaatgtt gtaaataatg ttagacaatt
    47041 tcctcttaca aatgttttag atacagataa taaagaatat agagaagcta caccaaatag
    47101 tgttaattct tctaatagca ctataaatct aggcggaata tctataaacg ttcaagagag
    47161 taataacaaa gaagaaatga tacaagaaat attatctcaa gtagaagatg gactaagaga
    47221 agcattacaa gacattggat aatgtcgaat tgttgttaaa aaaaactccc tcataaatga
    47281 tataatattg ttaaaatatg caaatttatg agggggaaat tttatgtggc agaaatttaa
    47341 aagtctaaat actttcttaa aagttattat aggtattata gcaatatgtt tcttaccaat
    47401 cactttagct gttttttcaa ttgaattttt aatgaaagct attgaagcta aaaataaagg
    47461 aaaaatagtc ttaggatgtt ttttagcatt attaacatta agttgtgcta ggactgttta
    47521 tcttacaaaa gatttagatg ataaaaattc tactcaagtc aatcaagttt ctagtaataa
    47581 agataaagaa gatgctgaaa agaaagcgga agaagaaact aaaaaacaaa aagagcagaa
    47641 ggctcaaaca gagaaacaaa aaaaagatga agaagctata aaattagtag aacaacaaaa
    47701 aaagaatgaa gatgagcaaa aatcattgga aaataagaaa aacaatgaac caacaaagtt
    47761 ttttaatgat gtatacttaa aaaataaaaa ttgtgttaac atggcatctt atgaaggaat
    47821 aaaatcaaac atagacatat ttggctataa atataaaatt atagaaccaa acgaaactac
    47881 attaagacag attgatgtat atgacaatca aagcaatgat actgttcaat ttcaatttta
    47941 tgaaaatgat actggagaag aagtcgtaac agttatgcaa tacaataaaa aagatgaacc
    48001 tgacaagtcc gttgctataa gtgatgattt tcatatatat ccaccaaaat ttaataatca
    48061 tattaataat acaaatacag aagttaatag catagaagaa caaataaatt ttatgtttaa
    48121 ttgatatatg tataatactt taaagctctt acaacttgta ggagcttttt gttgtaaaaa
    48181 agaggtgata acttggtaat agacatatac ctaaaaaatg aaaaagaaaa aatagatttc
    48241 cattttccag taactccact tgatagttta tctataaaga aagaaaaaag atttgaaact
    48301 gttgatatag tgaatttagg tgaatttgac attaaaaaag aaggagagaa gataagagaa
    48361 atatcattta aaacatttct accttttcaa tacgatgctt cttattgcag atatagcgag
    48421 ttaaaaaatc ctactgaaat agttgcaatg cttgaaaaat gggtagacca aagcgaacct
    48481 ctaagactta taatgactgg ttttggttac aatggattag ttacaatatc tagttttagc
    48541 aatactcaaa cagcaggaag agaagaagat agagacattg agataacatt tagaacttat
    48601 agagaattga agatagagac attaaaaaaa gaaactaaaa gtaatactaa aacagattta
    48661 aaagataata gacctaacaa taaaaccact actaaaatat atactgttaa agcaagtgat
    48721 acattatata agatagcgaa aaatctttta ggtaaaggtt cgagatggcc agaaatttat
    48781 aatatacctg aaaataaaaa aactattggc aaaaacccta atgtgataaa aaaagggcaa
    48841 aagttggtga taccttctaa atgaaaataa tattaaatgg aaaatatgat attgcaaatt
    48901 ttaatgaagg aataacatta agtgaagcta tagacggagt tgcatataag atggatgtat
    48961 cattagtaga acctaagcaa cttcaagata taggaattaa aaaaggtgat aaaatagttc
    49021 taattgacat tgcgtatgag agtaaaaaag aagaaactat atttaatgga gtcgtatggg
    49081 aaactagaag aagcgaaaag agtaaaaaac tgaccttatc ttgtagagaa agaacagtct
    49141 atatggaaga atcagaagaa caatatcagt ttaaagaaaa tacagctaca cagaggattg
    49201 aatactactg taaacaatgg aatataccat gttacaatct agctaacaca ggaaagaaac
    49261 ttgctaaagt aatacataag actaacatac tagatatgat taaaaaagac ttaaaagaaa
    49321 cagctagtaa gggtggagac ttatttaggg taaggatgga taataagttg aaattgttta
    49381 agcttggcac taatgcaaat gtatataaat tagatagtat attagaagat gcaaacttta
    49441 caagtagttt taatgatgca gtaacaagtg taaaagtgct aggtaagagt aaagatgaaa
    49501 atacaaaagc acctatcatt ggcacatata agaaggactc agataagtat ggaacactac
    49561 aaaagattaa acaagatgaa aagataaaaa atgctaaaga agctaagaaa gcagcagaag
    49621 caatgttcaa tagtggagag gaaacaataa gtgtagattg tgcagtggat ataaatagaa
    49681 tacgtgcagg tgataaggta agtttaaaag gaaaagaata ctatgttata gatgtcactc
    49741 atacattaga ctctatacca aaaatgaagc tcaatatagg aactttagat tatataagga
    49801 ggaaatttta taacaatgac tgatgctaga ttcaatagag ttgctagaat attaaaagaa
    49861 aaaatgaata aaagtgttaa tgatggagtc tttggaatgg gttgttcact tgcagaaata
    49921 acaacaaatg gtctgaaagt aaatggttat aaagatgaaa tacaggacta tttagtgtta
    49981 gagaatttaa cattaaaaga agattatttt actctttcag atgaaatatt aggtgcagaa
    50041 tatagacata aacataaaat agaaactccg aaagagttga aaccattgac tataggtgat
    50101 aatgtattag tagccgttat gggaactgaa tttgtagtaa ttgggagggt tgtaaatgcc
    50161 aaacctattt ccacaaaatg aaacttttga aactgtagaa ttaaaaaata ataatgaaaa
    50221 tgaactggac ctaaaggggt cttttttatt tgattttata aaaggcgaat ttgtcaaaaa
    50281 tgcagatgga acattaaaaa gatgtaacaa ggtagaggca tataaacaat ggtgtcaaaa
    50341 agctatatta acacctagat acaaacggtc agcttattct agtgtatatg gaagtgaaat
    50401 aaaagactta attgctagta atctatctca aagtgctaaa gaacttgaaa tatctaggtt
    50461 aataaaagaa actattttag ttcatccata tacaaaagaa gtgagtaatt ttatattcaa
    50521 ttggcttgaa aatagtaggc ttgttaatta tgaatttgat gtactaacaa tagatgatga
    50581 aaatatagta attgatggca atataaaaag gtaggtgatt atatggaaag agaactacct
    50641 ataccagtat ttttaacgga agatgaagaa gtaatacacg aaaggatgtt aagtaacttt
    50701 caagatgtga gcacactaga aggggacttt atttatgatg caacaagacc tacagcagaa
    50761 gaaatcacac agttaaaaca attaggatta caaaataatt taaagattgc atttcctcag
    50821 acatcttatg gaacttattt agagtggctt ggtgaatgta aaggagtatt taaaaatcaa
    50881 ccaactaaat cggttggcat ggttacattt acaggcgtac aaggagctat cattgcaaaa
    50941 gggactattg ttacaacgat tgctacagat gaaaaacaga gcatagagtt tgagcttctt
    51001 gaaactaaaa ctataggaga aaatgaaaca gtagatatta aagcagaatg caggattgca
    51061 gggattatag gaaatgtatc taaaggcagt atatctgttt tattaggctc tattaatggg
    51121 attaaatctg ttaataataa agaagatttt aaaggtggaa cagatataga agacgaagaa
    51181 cattttagag aaagagtcct tgtgtcagaa caagaggacc gattaagtgg agctagttca
    51241 gattatataa gatgggctaa agaagttgat ggagttggat atgcttatgt agtttcagaa
    51301 tgggctggag caggaacagt aaaagtatta atactagata aaaatagaaa agcagcaaca
    51361 caagaattaa tagataaagt tcaagaatat atatatccat taaatgtatc agaaggagaa
    51421 aatagagacg ggaaagctcc tattggggct ttagtaacag ttgtaacacc cgaaacacta
    51481 cttattaatg taaaagctag ttttatattt agtaacagtt ttagtgaaga aacagtatta
    51541 aacaatctaa aagctaagat agacaaatat ttagataaga ttgatttagg tggaacagtc
    51601 tcatacaatg ctatacaggc aatagtaggt tctatgatgt tgacagacga aggtatacag
    51661 gacttttcta atcttactat aaatgatgta aaagaaaata taaaattgca ggaccaagtg
    51721 gtcggagtag gggaaatagt taacgaggtg gttggatgat agcttctaaa aaaggtagag
    51781 aaatgcttct tacattgtca ccaatatatg agcaatctat tataatgcaa agcttatatg
    51841 aagctatagg aagcgaattt gataatctag atttattaaa taaagaaata gaattacaat
    51901 tatttcctca gagtgcaaca tggggacttg aattttggga aaatagggta ggtttatcta
    51961 ctaatctaga tgaagctata gaagctagga gaagaaaagt tattgctaag ctccaaagca
    52021 aatatattat gacacctaaa agaatgtcta tgatacttca atcttataca ggtgcaaata
    52081 taaaaataaa tgaaaacata tctccatata cttttggtgt tgaattaacc agtactcaag
    52141 gttttcctag agatttagaa gatttatata agagagtaaa tgttataaaa ccttctcatc
    52201 tatctgtaag ttataagtta gtatctttat taaaaagtaa aacttatttt gcacaaacaa
    52261 caattatgag tgaagaaata acagtatatc cttattcaag taaagaagta aaagctaata
    52321 ttaaagctaa gtttgcatta gctcataaca tgagttcaga aatattgaca gtatatccaa
    52381 aaggaggtgg tataaatggc tgatgaacaa ttttacacta tacttacaaa tataggtaag
    52441 gctaagattg ctaatgcagg aatgttaggt aaatcagtag tattagagaa gattcaagca
    52501 ggtgatggag gaggaagcta ttacaatcca acagaagacc aaacagcatt aaaaaataaa
    52561 gtttgggaag gtaatataaa tgtttttgac aaggatgaaa ataatcctaa ttggattatt
    52621 gcaacagcat gtgtacctgg ttcaataggt ggatttacag ttagagaaat ggctcttata
    52681 gataatgaag gcgatatgat tgcaatttgc aaaagtcctg aaacctataa gccaaaagtt
    52741 ggcaatggag ctatgaaaga tttgtattta aaatttatta tagaggtatc taatgtagag
    52801 aaagtgacct tagttgttga cccaactgcg atatttttaa ctaaaaaaga tgaagaaaaa
    52861 atattaacaa atatcaataa gttagacact aaaattgata caactaaaac agaattaaca
    52921 agcaacatag aaactgctaa aacagagtta aacaacaaaa taggagatac aacacaactt
    52981 actacaacag ataaaacaaa tatagttagt gcattaaatg aggtaaaaac tagtgtagat
    53041 agtatagaaa caacagcaga gaaaacaagc tataataatg caacaagtaa tcttactgct
    53101 acaaatgttc aaggggcaat agatgaaatt gttgcaaaaa tagaaaattt taacgagatt
    53161 aatatatcca tacaaaacga catgttacct atttaagaga ggagagtgaa aaatggctat
    53221 agtttatgaa tttaattata caggtgctga acaaagtgta gtgttgccac ctggtaaata
    53281 taagtttgaa tgttttggtg cttgtggtgg taattattat gattttgtac agtgcgcaaa
    53341 aggtggctat actgctggtt ctttaatatt gaaagaaaat actacattac atgtttacgt
    53401 tgggcaaagt ggttattgta aaggtgttaa tggtattgag acttgtagaa gtggttttaa
    53461 tggtgcgggt ggtataacca cttataaaag tacttctgat ggttattata gtcttgcagg
    53521 tggtggagct actgatatta gacttattgg tggtaactgg gataatttac aaagtttgct
    53581 atctcgcata attgttgcag gtggcggtgg tggtggaagc ggaaattcac atgatagtat
    53641 tggtcatggt ggtggtacaa aaggtaaaga tggtatttct atagcaaata aatattttgc
    53701 aggcggtgga agccaatttc aaggcggttt aacttttaat agtctttata atggttcttt
    53761 tggtgtaagc ggtgctggag atggcattag tggtgttggt ggcggtggtg gttggtattg
    53821 tggtgcaggt agtttttatg ctgaatttgg tggtggtgga agtggatata ttctaacaaa
    53881 agatagttat aagccagcaa attatagtcc atcttctaaa tattatttta gcgatataaa
    53941 tagcgttgta ggtggaaata ctacgaaaca agatggttat gctaaaataa cattactcca
    54001 agcattacct tttttaacta tatcctctta taattccact acagctacat ttaaagctga
    54061 ccacacagac cctacattgc ttacaaaaat agaatatttt atagatgatg tactaaaaga
    54121 aactataaca acagatttaa caacagagaa aataattaac tatacattag aagataatgc
    54181 actacacaca cttaaaatag ttgttacaga cagtgctaat gctacagcag aaaaagttgt
    54241 aagtataagc aaaggaattg cacctcttcc tgctggttct acaacagatg aagttacaag
    54301 taaatggata gaaattaaag atacattcaa aagtggcaaa acaagtatta taaatacttt
    54361 ggcactaaag aatatagaag caagtttaaa taacacatta gtcgagttgt cagagaaaat
    54421 aaaaacaagt tttgatagtt ctgacgctag tgtgcaggat ttgatgaatc aattaacaca
    54481 agctaataat accatagcac agttaaatac aaaatataaa gtcgcaagtg gcacagttac
    54541 tccttttaag ggtgatgaca ctaaaattgc ttatccatat ctaactgata gagtttttaa
    54601 acctagcgct tgggttaaaa ttagtaactt agattttaag cctaatattt tctttgctga
    54661 ttttgattac tatgatactg aatataaaaa taattataaa cttttccttt tcgcttgttg
    54721 tggtgttgct acacaaagag gtgttgattt ttcaagtgtt acatcttttg ttagaaaaag
    54781 tagtgatgag tattttcatg ctaatggctg gctctataat aattccgaag gagatgttta
    54841 ttttaataat actggtgttc aaattccagc ttatagcttt gactcaactc aaagacatat
    54901 ttataaatgg tatgctatta aattcattta aaaggaggaa aaaacatgaa tgttccaaac
    54961 agagtaatat atgaccagac aggtagaata atctttgaaa caggcgagtc ctgcggggat
    55021 gtgttgccac acgataaaat aactgaactg cattatatag atgttgaata tggaagtata
    55081 gattatacaa gaaataagat tgtaggcata aatatagaaa caaaagaacc tgtcttagaa
    55141 gaaattccag catatataag taaagcagaa aaggaaaaac aagaattaga aaatcaatta
    55201 cttttaatga caaataaaga actaggtgga ggtattttat aatgaatata aataatgttg
    55261 tagtaagaat attagcagaa agaattttaa acaaaggact gaaccctttg aaaaacagac
    55321 cttttgagtt agatgacgtg actaacatag agtacagaaa agcagtagat gattatataa
    55381 atacaataga acaggaaata aaatagtaac ttttagtaac tcttcttaaa gaataggtca
    55441 ctaaaaaatt ttaattttgt ttattttact tactttcaca tacttataaa gaaatcattc
    55501 taaattctac attaaaagaa acgttcaaat ttaatcatct ttctacttta aaagtaacct
    55561 attctttaaa aatggttact aaaaatagaa aggtgatatt tttatgatag aaaaattaaa
    55621 cgaaaatgct agtttaagtg acttaataac cgcttttgaa aatagtacaa aagaattaaa
    55681 agcaagtaaa gataatttta caaatttgct aggcaatcca tttttagaaa gtactaaatt
    55741 ctcggagttt gagggtaaaa tgcaaaatct tatgagtact tttaaagata atttaaaaag
    55801 taaaggcata aatagtggta atacagagtc tttattgtct ttaattaata aagttgcaaa
    55861 tatatatata cctacgccga tatatacagc aagtggcgag tgtactattg ataataaagg
    55921 tacatgcaaa agatatacaa ttagtacagc tggagacgtt tactacgtta gaattaactt
    55981 aaatttttat ccgaaattta ttgttatgta taactccggt ggagctagta aatattttac
    56041 aaccagctat ttttgtagtt atgttccttt cattgcttca acagtagaaa gttatacgac
    56101 ttacttgctt gataaaagcg atagtaggat acaagttcaa aaaggaacat gtattttacc
    56161 tatagtttac agcaagtatg gtagttctag tttttatttt catgcggtag gttaaggagt
    56221 ggttttatga atgattatat agaagtagga agaaggatat tttttgatga agaaggcgaa
    56281 ataatattct atgaaggaca atcaaaaggg aatgttcccg aaagaaaaaa tattaaaaaa
    56341 atagaatata tagatttgga atacgattat gttgattatg ataagtacaa aataataggt
    56401 ataaatatag agactaaaca gccaatttta gaagaaatac caatctatat gagtgaagaa
    56461 gaaaagagaa tacaagaatt agaaaatcaa ttactaattg cagaaaatga aaaagtagga
    56521 ggattattat aaagaacaca ataatgttgt agtaagaata ttagctaata aaaaatagcg
    56581 aagtagtaaa agaagcagaa ccgtataagg ttcttttttt atagaattag ataatttata
    56641 agttttattt tgataaaaaa acggaggaaa attatggaac aattattaac agaattaagt
    56701 agtttaggag caataggcat attatgcgct ttgctattta aaaataccat gcaggagaaa
    56761 aaagaagata gagatatgta taaaaagaca gtagaaaatt tcatagaact atctacacag
    56821 caacaagaga taaataagaa tatacttgtt gaaatgggag caatgaaaac agacgtagaa
    56881 gaaatcaaag aagatgtaac agacataaaa gacatgttac agaaagaagg tctttaaatg
    56941 aaagtagcaa tagtgccagg gcatacacta acaggaaaag gaacaggagc aacagggtat
    57001 ataaatgaag gaacagaaaa caggatattg atggatttgg ttgtcaaatg gcttaaaaag
    57061 ggtggagcta ctgtatatag tggtaaggta gataagtcta ataactactt agcagagcaa
    57121 tgtcaaatag ccaataagca aaatgtagat gtagctgtac aaatacattt taacgctgac
    57181 catacaactt tggataaaat gggtacagaa acaatttata aaactaataa tgggaaaaaa
    57241 tatgctgaca gagttaataa gaagttagcg acagtattta aaaacagagg tgcaaaatca
    57301 gatgtcagag ggctttactg gcttagtcat acaaaagctc cagcaatatt aatagaagtg
    57361 tgtttcgtag atagtaaagc agatacagac tattatatta gacataaaga catagtcgct
    57421 aagttaatag cagaaggtat tttaaataaa aatatagata ataaagaaaa tagtgaggat
    57481 aagaaaatgt ataaacatac aatcgtttat gatggagaag ttgacaaaat tcctgcgact
    57541 gtagttggtt ggggctataa tgatggaaaa atactgatat gtgatataaa agattatgta
    57601 ccaggtaaga cagaaaattt atatgttgta ggaggtagag catgtgagaa gattggttct
    57661 atgactaaag aaaaatttac tatgataaag ggtaatgata gatttgatac actttataaa
    57721 gcattagatt ttattaagaa ataagtttgt agagagtggt aactatttca tcagttacca
    57781 ctctctattt ttttatcctt tttgttatat tctctttcaa gtgctgtttt tttaaaaacc
    57841 catgtcgttc cgaatttttt acagtctaca ccttccacaa attttccatt tcttatatta
    57901 agtcttaacg tactttcacc ttttttataa agttcacaag catcttttat agagattagt
    57961 tcctcaaata catctttcat tttaacaact ccttttattt atattataat cgcaaaagtt
    58021 taaaaagaca agaaaaaaat aaacgtttat gtttaaaaaa ctattgactt aataaacgta
    58081 agcgtttata atatgattaa agaaagataa acaaaggggg atttaaaaat gaaaaaaata
    58141 actaagaaag aaattaaaaa gtacgttaga gaagcggtta ataacaattt taactgggaa
    58201 ataaataaat gtgggtttta cattaaaaat aatgaaatag aattttttat ttcttacaag
    58261 ggtcaaggaa tagatgaaaa tgtatataat gatacttatg aagaaataat ttacattgag
    58321 gatattatag aagaatacag aagaaaagaa tacaatttag aagatgtaga ttctattgta
    58381 tatgacaatg taaatgatat gattaattgt tataatgaag aaaagtaata aaaataaata
    58441 cagtgagttt aatcaaattc actgtattta tttttacaat agtagaataa tattattagt
    58501 tcaggatgtg ataggcttga aaattggaga taaattcgaa aaccttacaa tactagacat
    58561 agaacaaaaa aacagtagga aatactgtct atgtaaatgt aaaaattgtg gcaatgaaaa
    58621 gtggataaga gcagatagtt taaaaagaat taaagggtgt ggatgtttgc aaaaagaaac
    58681 acagtttaag caaaatgatt tgacaaataa aaagtttgga agattaacag cactaaaaaa
    58741 cactaataaa aaagctaaaa gtggtcacta tatttggatt tgtaagtgct cttgtggaaa
    58801 tgagattaag acaacagaaa acaatttaac tactggcaga actaaatcat gtggatgttt
    58861 gaaaaaggaa tctaatataa aaaatgcaaa gatagcttta agagtacatc aacaaaaaaa
    58921 tattgttgat gatacaaata tatctattat aaaaaagaca gaagcaagta taaattctaa
    58981 aacaaaaata agaggagttt gttggaataa agaaaagggt aaataccatg ctcagataga
    59041 gtttaagaaa gtacatcgca atttaggata ctatgataat atagaagatg ctgagaaggc
    59101 atatatggaa gctaaagaga agttacataa gaaattttta aaagataatg acaagttaaa
    59161 agaaatagaa tattagatgt aattgaattt attttaaaag attataaaat tgaatataaa
    59221 aatcatttat aaaattgaat ataaaaatca taaagctgta ttgttataat acagtttttt
    59281 aatacaaaaa tacagatata tcttgaataa tattttaaaa taggaggaaa ttaaaatggg
    59341 taaaatggat aaaaaagaag ctaaaaaatt tttaaaggtt gtagatatga atatagataa
    59401 aataaaggaa gaagctacga aaatttataa agaatgctac ttaatagaaa caactgatac
    59461 tataagaatt tctataaatt tgaaaggagt tgtaaaaagc acaatttcaa gttggcaagg
    59521 ctattcagat gatattttta atatgagaga aattattgtc tatgaattta gtcaaggaga
    59581 agttcaaatt gaagatcttt taggagaact ttgtttttta aatgattacg aagaatttgc
    59641 tacttggtgt gacactgaaa gcgaaacttt gaattggaat agttatgaaa aattcaacaa
    59701 agaaaatttt gacgaattag tcgagagaaa tatagaagat agtttaccag attttttgga
    59761 agaattgagc gaaagtatag aaaattgtaa acaagaatta aaatcaatga ttgaaattta
    59821 aatgaaagag tattattatt aatgtatata gtatcgcttt tgattaacta cgagggattt
    59881 aaatgtcaat ttaatttata tcgaaatgat ataatatcca attttagatt aactatacgg
    59941 aaataaaagc agtgtattca ataaatacac tgctttttta atgctaaagt tttagttatt
    60001 atgataactt aatccttcga ctataaatcc tacattcgtt gtcatttcca attcgttact
    60061 taacattatt ccttgcgtaa attcactacc agctggcaat gatacttttt ttcttgcaac
    60121 cgcttctatg attgtttgaa atagtttaat tttcatattc ttatcaagtc cagcgtattg
    60181 caaatagttt gctacttctt gtgaaaattt agttaattct gatttagtca ttttttcaga
    60241 taactctttt ccaattcgca aataaacatt tttagattgt tcaacatcca tttttttcaa
    60301 ccccttttta gattataatt aaattatatt ttaaaaatgc taatacttca aatataaata
    60361 tttttgttta agtatgtaaa catcaataac atcactcaac acaccttaaa attgatttaa
    60421 atagctcttt ttgatgcaca aaattaatga tataataaaa aagtagatat gcaaccccaa
    60481 cgcatattta ctgagtcaaa cattattata tagagcattc ttcgttgtgg agagtgcttt
    60541 ttcatttctt tgaataacta tgttgattat ttataatatt ttcatttaat ttatctttat
    60601 ctatcaaatt taatgcacaa ttaatacaaa tgttagtttt catatgactt ttcttatgaa
    60661 aagtaatata cttattatca tctttatcta ttcctttatt acagtaatta cataatatag
    60721 tcttagtcat atttttattc ctttcatttt atattttcca taattaagtt taacatattt
    60781 agtatatacc tatgtatata tatatatata tttaaaacaa tgttttttat tgcaatatag
    60841 aatatattgt ataaataaaa aatatgaaga atatatattt gataattgta aaattatgtt
    60901 ataatttata tgtaaggaac taaatatcta agagtggcag tttccacatt atgctctaac
    60961 ctactttcta atgaaaggag gtgggaagtg tggaaatagt agcagtttat tacatagcaa
    61021 agtttttact tgatttatac aaagtaaaaa gccactcaaa gtcggaagct aagagtggct
    61081 ttatatttaa tttaactatt aataaactaa tagtaaaaat taaatataaa aagtactaaa
    61141 ttttttcgga aactacactc tcgacaatag attatagttc cttatttcta aagaggtagg
    61201 agaattacag ttctcttatc tctttttgat acgaatttac atctttatta tatcgcattt
    61261 taacaaaaaa taaaactaag aatattataa atctatttta attatttttt tatcatatca
    61321 ttatttaaaa gtagataaaa ctaaaaaggg tgttctagaa aataatgtct atctaattat
    61381 acttacttac caataccatt acttgatgta agcaagactc tactacattt tctccatact
    61441 ctagtataaa atcatctagt tgtgtatcta taaaatcaca atagtcatat gaactaccaa
    61501 tataagcagt caacatgtgt gtgcatttat ccattttatc tctaaacata tatattatta
    61561 tccccctaaa aaactttcat aaaagcaaaa agaacaatat ctttttggca actggctgac
    61621 ataactcata agatgctata taagtcttag tccctatagc tttgcgtcac taaatttctc
    61681 tagttttgcc gatttagttt tactctacaa ctacaataat acattagaat gagtcattat
    61741 gcaacatgct tgtttgaaaa ttaaataaat ttatagatat gaataatatt ttttgtatgt
    61801 aaaaataaac taaaaacgta gaatgcatgg taaaacttac ctgtagtttt tgtcctaaaa
    61861 aactcctatt attaacttaa attaagttta atttagggaa gtgacataaa atggaacaac
    61921 aagaagtagt taataagctg cgcaggggag aactaatgtt aatggaatac ttgtggagga
    61981 aaaagtctat tttatctaaa aaagaaataa tagaagcaat gaaagatagg tataagtgga
    62041 ggaaaagcac tacagaaatt cttttaaaaa gattagtaaa gatgaaaaca ttaaaaaaga
    62101 agaggatagg ttttgaatta aattatgaag tgctagtaac taaaaaagag tatttaaatg
    62161 ctagaaaaga agatagacag gtaactaaat atgatagttt ttttatacga atgtttacta
    62221 ctatgcataa aaaagaaaag atgacagaag aacagataaa aaaatatata gaaaatatgg
    62281 aaaaaattgg taaagaataa tttatatata ttaattattt ttaaaataaa tttgttataa
    62341 tatttaaaca tacataaatt tacaaagggg gtgtggctta tgggattcaa gaaattgcca
    62401 gattctgagt taaaagtaat gaaatttatt tggggattag ataaaaataa ggtagcatca
    62461 ggagaagttg tggaagcaat gaaacaaatt tatgactgga ataaaaatac aacattaaca
    62521 atattgtcta agcttgcaaa gaaaaactat ttgtattctc aaaaaacaag caagtgtaca
    62581 tattatacta tcgctgtaag ggaaaaagat tatttaaaag ttgaaacaaa aaaatttttt
    62641 agtttttttc ataataattc tttaaaaagt tttttcactg ctttaaatga cgaagaagac
    62701 ttaagtgatg ataaattaga tatgttagag caatgggtaa aaaattggga agaggatgaa
    62761 taaaatgaat gtgatactaa aatgatacta atatgtttta tagcgtgtta aaaaatatag
    62821 aaataataga aataatagaa gaaaattcat gtaatagatg aggttttatc agattaaaaa
    62881 gaaaaacaat agaatgggaa taaaatatag aatgccagaa atccacatga atacaggatt
    62941 tgaggtctct cttacttcta tattatactg gtttgatact atcaatattt gccagtatct
    63001 tatggattaa taagcaaaaa acatcctgct gaatggtaaa ttcggcgggg tgtttttatt
    63061 ttgttttgaa actcctttgc tctgaaactc gaagtattta acactgatga tgttgttatc
    63121 ttttagttcc ttttgtgtca tgctctgaaa gacaaattga tacaataaaa aagtaatttc
    63181 ttaatcattg atggagatat gtatttatgt tactcttgat ggtaatttga gtaatattgt
    63241 tcatagcttt ttatattcac ttaattagaa aaccatttgg tataatcata aataaacttg
    63301 atgatataat agaaaagatt tataattatt agaagtatat aaaagtattt tatgtctatg
    63361 aaatgtatgt ctataaaaca tatagatgac ttttgtatat taatgtatga atattgatat
    63421 tatagaagaa ttgagttgtc aatgatacta aatcaaatat ttatagaaga tggagggatt
    63481 atgagaaata aaataattat aggaattact ttttcacttg tattactaat gtctggatgt
    63541 gcttcaaaac aaacagcaga aataaacgta ttagcaacta ctgatttaca tggagttata
    63601 ccttatgagt taacttctta tgtaaaagaa gaaagaaaaa aagataaaaa tctaacttta
    63661 gtagatgcag gagatttttt tgatagtgga gaagtgtttg gttctagcat ggatagttat
    63721 tttagtgcta gatataaaga tggtgataat aacactgaaa gttatattga gacgcctatt
    63781 tcaaaagata tgaaagaggt aggttatgat gttgcagttc ttgggaatca tgaatttata
    63841 tccaacaata aattttatct agataatatg atatctgatt ttgagaaaca aaaaataaat
    63901 attttatcgg caaatacgta taaaaaaaat ggagatagtt atacaaaacc atacataata
    63961 aaaaatatta atacacctga aggaaacgta aaactaggaa ttttagggct tactataaaa
    64021 gaagttggtg agaggaaaca gtggaaagat ggaaaacttg ttgactctaa gtctttagaa
    64081 ctaaaagacc aagagggcta caatggtgaa ttgtatatga atgatttagt agaagatgct
    64141 aaaaagtggg ttggtataat ggaaaaagag aagcctgaca ttatagttgc tgtggcacat
    64201 acaggtgaga aacctaagaa acctagaaac ccaggaaata gaatacaaga cttagctcaa
    64261 caagttgatg gaattgatgc tatagttgca ggtcataatc atgttcaaat tgagcaacat
    64321 gattataaaa ataaatcagg agaaaatgtt atagttactg agccaggaaa gcatggggaa
    64381 tatatttcta agattaattt taaattagaa aaaaacaatg ataaatggaa tatagttgac
    64441 aaatctagta aattaattaa atttgaaaaa aatcaagaaa gtgataaatt tggagaattt
    64501 atttcttcaa ttaataaagt aagtaaagat aagaaagaaa taagattatc agaagtaaag
    64561 actcctgaat gggataaaat atatgttttt aaatctggaa catctgcaga tgatatatat
    64621 aaaacaatag gatataaatg gagaaatata tcatctccaa agggtgatga catggtgcag
    64681 attgttgtaa tgaataataa taagcctgtt agctatatat atggtgaagc tgaagttatg
    64741 ccaattaata taaaatgtga tgaatcagag tataaaaata atgttgtaac tatttatcct
    64801 aataaaaatg ataaatttaa aattgaaaaa ggtaagcaag aatatagtat taatttaaaa
    64861 catatacaat agaaactttt atcttattta agtttagatt gtacttaaaa taaagtgtgt
    64921 attattttaa aaagtgatga taattaaaat cacaacatta aggaatagta cacacttttt
    64981 atgctaaaaa atagaatgtt aaaaaattaa acctttaaat taaagtacat tgcttatatt
    65041 atccaatatt atttccaaag gttgtgtaat atttttatcc ttatgtaata ttgctaacat
    65101 tttattttcc atatctatct catctaattc aatcaatcgc aattttttat gtaagagtga
    65161 atctaataat aaattttctg gcaaaatggt tatacctaac cctgcttctg cagctttaat
    65221 aagtgcaaaa gaattaacac tttcccaaac aggataagct ttttggtttg aaagagataa
    65281 tacactgtcc aaagtatcgc gaatagcact tccttgttca cgtaacaata acggaaacgt
    65341 gcataactgt cgtagagata aggtttgttc agataaagga aaagttggag cacatgcaac
    65401 acataactta taggaaccta ataaaattgt acggaatgcc ccctgtggtt ctataccttc
    65461 ccaaaaacat atatcagctt ctccactttt taaaatatcc atagctgaat tagcactttc
    65521 gacacgtaca ttcactttaa gagatggaaa tgaatttttt agtcgactta aaattggtgg
    65581 aagtaaaaaa gaggcaattg taatgctaga aacaatgttt ataggtgtac attcttctaa
    65641 acgactgatt tttttgtcta aattgcgaca agcagttaag atactacgtg cttcatctaa
    65701 aagagaaatg ccacagcgag taagacatac ccctcgtggt aatctgtcaa atagtgatgt
    65761 ttcagcttgt tgttcaagtt cagctatagc atgagataca gcagattgtg tgatatgtaa
    65821 tttttttgca gcacctgtaa aagtgcctgt ttgttcaatt gcttctaaaa tctctaaata
    65881 tcgtatgttt atcataataa atatcctcac tatacatgaa taaaattaat taattccatt
    65941 aaattctatc attttacaga ttgcaaagca acaggtatac tgaataatat gaagtgaaaa
    66001 gaaggaggaa tattttgaat caaagcagaa aaatatattt ttggctttta attattaact
    66061 tgtttattag cacattcact tttggcggag ggtatgtagt tgtacctatg attagacgtt
    66121 attttgtaaa tcaaaagcaa ttctttacag aagatgattt gatgagtatg gcagcagttg
    66181 cacaatcaac tcctggagct attgcaatta atttatctgc tttggcaggt tatcgagtag
    66241 ctggtacttt aggggttgtc attagttgta cagcagctat aatacctcca ctcgttattc
    66301 ttggattagt atcagcattt tacaccactt ttatatctaa tgccattgta actgcagtat
    66361 taaaaggaat gcaagctggt gttgctgcac taattgtgga tttgattatt gacatgtgct
    66421 ctatgatatt aaaacaacgt tctttgtttt tatcaactat gatacctatt gctttctttg
    66481 caaactttgt gatgagtata aatgtaattt ttatattatc tgtttgctgt tttgtatgtg
    66541 taatgcaagt tttctataag aagagggaaa ataaatgaat acaatttgga acttattttt
    66601 tatattttta aaaataggct tgtttagtat tggtggtggt tatgcaatca tacctttaat
    66661 ccaagagaaa gtagtaaatc aaactggatg gataagtgag aaaatgttta cagatattat
    66721 aactatttca caaatgacac cagggccact ggctgttaat acttctactt ttgttggact
    66781 tcaaattgga gggttttggg gagcagttgc agccacttta ggttgtgtat tatgtggtgt
    66841 agtaatatct ctatttttat ataatttttt tcaaaagcat cagaactcta cttatatatt
    66901 tgaaatatta aatggtctaa aatcagcttc attaggttta attatctcag ctgcggctac
    66961 aattataatc cttgcattat ttgggacgaa taaagtgtac ttcaattcta tatttgagat
    67021 gctaaactgg actgcactaa taatcttttt tgttatacta ctgatagtac gtaagtggaa
    67081 aattaatcct attgtgatta taataataag tggtatacta ggaatatttt tttatttata
    67141 agtttttggt aagtaaagga tatctatttt tttatatctt aaatccaaat agccaataaa
    67201 gtaaggctat ttggattttt aataaattct gaaaagataa tcagaaaccc atataacatt
    67261 taaattatat cactggtttt ctacaaacca atcataagct tcttggcaaa atttctcact
    67321 atctatatag taactattat gaacattatt aatttcaaca agtttctttt tacattttag
    67381 tgaattataa tagctatcaa aatattgggt tttaaaatat ctatcatgaa gtgttttaaa
    67441 gacaagagta ggtatgttga ctccatcttt tatcttacta tcttctgcaa taaagcattc
    67501 taacattgta ttaattatct ttgtactatt tttccaaata tgaggatttt tcttttcaaa
    67561 tattttataa aattcattat catagacttc attataacct ttatatttag gtggttttac
    67621 tgttatgtga aaattaggaa gaaacttaaa gaaaaaatat aataattttt atgttctgtt
    67681 ttatatatta ttcatatttt atcacagata atatactaaa gttaattttt ttattgtaca
    67741 actattataa taattcttct ataatataag acattctata attttatatt taagttcatg
    67801 tgtatttaat tgtttttata taaaatgtcc agtaatacta tttggattta tttttatttc
    67861 atcaggagtt ccagaagcaa caatttcccc tccatttatt ccactgtctg gacctatatc
    67921 gataatccaa tcagaagcac gtataacctc tgtattgtgt tcaataatta aaagtgaatg
    67981 ctgttcattt acaagttttc taaaagtaat caacaaattt tctatatctt taaaatgaag
    68041 tccagtagta ggctcatcca atatgtacag cattcgtttt ttagtttttg tccctaattc
    68101 ttttgctaat tttatccttt gagactctcc tccgcttaat cctaatgtat tttgtccaag
    68161 ctttagatat ccaagtccca cttcatctaa taaggagagt tttctatata tatcagagca
    68221 atcactaaaa aatgtttttg cttctgctac ttccatgtca agtatatctg caattgtttt
    68281 tcccttgtat tgtatagaca gtatttcttt ttgatatctc ttaccttgac attcattaca
    68341 agttacccat tggtctgcca tatattgtag aataattttt gttttaccta atccttgaca
    68401 gtttgggcat tgcccctttt tgctattaaa gctaaaatag ctttcatcca ataatttttg
    68461 ttttgcttgt ggagtttcag taaatagaga acgaatttca tcaaaaatat tgatatatgt
    68521 agcaggtgta gaacggctag attttccaat aggtgtttgg tccattaaga taaaatcgtc
    68581 aatagcatca aatccagtaa gtgcagtata atttttattt gggatagatt tttgtttcaa
    68641 tttttcttct agagcaggaa gtaaactgtg aaatacaaga gaactcttac cagaaccact
    68701 aacacctgta atagagcaca tacagtttaa gggaatttct acatcaatat tttttaagtt
    68761 atttgcatgg catcccttta agcttaacca tttacttggt ataattttat tttgtgaact
    68821 tatcttattg tatgtactca aatatttgcc tgttatagaa tttggatttt ttataatttc
    68881 ttgggttgta ccttctgcaa tgatgaagcc cccttttgta ccagcatttg gtcctacatc
    68941 aataatataa tcagcagaca gtattgtatc cttatcatgt tccactacta taacagtatt
    69001 acccttgtct cttaattctt ctatcatttt gatgattaga ttatggtctc ttggatgtaa
    69061 accaatagat ggttcgtcta aaatataggt taatcctact aattcacttc ctagctgact
    69121 tgataatcgt actctctgca gttctcctcc agataaagtt ggagctgttc tatctgttga
    69181 tatatagcta agacctacct ttaatagata actaactcga atttttaatt ctgttaagat
    69241 atcatttacc aaagatagct catttttttg aagttgattt ggtaattcac acaaccaatt
    69301 ccataactgt tctatcgtca tttttgttag ttcagggaag cgataacctt tgattgtagt
    69361 gaatctagcc tcaatgcaca gcaattcgcc accacatgta ttgcaaggaa ttttgttcat
    69421 atattgatgc aaggtattat tttcagaact agattctcta aaaagtcttt gaatgtggtt
    69481 gattgctcct gaagcaggcc ttctaatctt tgattctcgt ccctttgatt caaagctaaa
    69541 ttcatatatc ttatcgtctg taccatataa gattgcatct ataaaatctc ttggcaattc
    69601 attccaaggt atatctaggt ctatattttt atcttttgct attgcatata attcacctac
    69661 catccaatta ccatttggct ttttccccct caattttcca aaatagtaag tagctccatc
    69721 taatattgaa atagttgggt cttttacaat taattgagga ttaacttgat aggtaaaacc
    69781 caatccatta caatcgtgac aagctccagt atgggagtta gcattgaagg ttgaagtatt
    69841 tatcttagaa aaaatagttt ggcaatgtgg acaataattt tttgtattat tctttttttc
    69901 taatggagtt ttgcaatatg gacagtaact ttttccgatt gatacaaata acattcttaa
    69961 gtaatcccaa atgcttgtaa gagtaccaac agtagaacga ggattttggc ttcctttttt
    70021 ctgttcgata gctatagtag gggttaatcc ctgaagtaaa tcaaattccg gtttttgaag
    70081 ctgttctgaa agttttgctc cattacctag attttcaaaa taacgtcttt ttccttcttc
    70141 ataaatcgta tcgaaagcta aagaagattt tccactacca cttacacctg taataacagt
    70201 tatttgattt tttggaattg taacctcaac atcctttaaa ttgttttgcc ttcctccttt
    70261 tagatagata gagcttttat tatgacaaag tgaattttta attgatttat ttattgcttt
    70321 tgcaaatttg aatttctttt ctagttcaag aggattgcct aattctttta tttccgaaat
    70381 acctaatatt tttaactgag aaaagattga tttcaaataa tcttcaggca atttttctat
    70441 taacaaatta aggtcttcta tttttatatt tccgcttgat aatatctctg ccatttcatc
    70501 taataaattt ttttctgctc ttgttaattg attgtatttt ttggaactta attctgtcca
    70561 taaatcccat ggaagcaatt ctaaaccaat catattatta aagtcatgta gaagattacc
    70621 ctttacagaa tgccaacctt tccaattttt attatatcca aagtcatctg gatgagcatg
    70681 tccttgtcga catcttatcc aggcttctga accagttaaa aaaccatctg tttttccgac
    70741 atcatgagtg tcaaaattaa tttgaaaata atttttttgt atgtcatcta tttgtgcatc
    70801 tattcttatc catctctttg ttagagtatc atagtattct acaagccagt ggtcttcata
    70861 agtaagctca ttttcaaaat aattagcaaa acctgctctc attctagcct caaaacctcg
    70921 atatcttagg agcgatacaa gaagtaaaga ataatctctg cacattccaa caagcctttg
    70981 ttttgggttt cttggaatag ttagtgggtt agaatttatt tcaaatattc tttttaacat
    71041 ttgaggaatt gtacgaagca attcctcatc agattgttgc ttattaaagg atataccata
    71101 taattttcct tggtctccat gaattactag gctttgaacc acttttaata actcaattgg
    71161 agtctttggt aaatcttgat atttattttc aaatttttta ggatttgtga tgatgctttg
    71221 atttttatag ttatttattt tcatttatat ttcacctctc taaatggatg atataccctg
    71281 acatggtgtc atatgcaagg acaatttaga ttttttggcc aaaatttaaa aataaataaa
    71341 aaatgataca gtgataaatt gtaatacaaa tatagtttca aattgcatat aatataatta
    71401 tttaataaaa ccattatcta tggaatgtta atgaaatgtt aagatggcat tcttgaatat
    71461 taagaaatag aatactataa tatagtgtga aggattctta gtaaaaaaat ttgtaagtgt
    71521 taatatacat taaaaaatag gtttataatt tggaggaaaa aaatgaaatt atcaaggtat
    71581 aagaaaaata gtttagttct gtctataatt ttatgttcac ttattatttc aatccttgta
    71641 attactgttt ttaataccgt aaaactacac aatatatcta ataaacaatc taaaaactat
    71701 ttaaacgatg tatctacaca gattgtaatg aatgttgatt ctaaaatcaa atttatttta
    71761 tccgacttaa gaataatggc agattttatt aagcaatatg aaggtgatag tcgatatgag
    71821 tatttatcta aaagaaagac tagttataag tattatgatg ttggaataat agatttagaa
    71881 ggtaaagctg agtttttaag tggaaaaaag tttgacttaa agaatactgc ttcctataaa
    71941 aaaggaattg aaggtaaaga atactcagaa gtaatagagt caatgggttt tgttctttat
    72001 agtgtaccta tatttaacga taatcatgaa gtgtctagta ttttaatagg tgtatccaat
    72061 aaagaaagca tggatgagat tcttaatata gatagcttta atggagaagg aactattgaa
    72121 attattaata gtcagggaaa aactcttttc ataggaaaaa ataatgagtt aataaaagac
    72181 cttagctata agtataagga ttctgcagat gaaccttggg cacaaaagat gcttaatgat
    72241 tttaaaaatg ataaatcagg aaatattaca ataacctcat caaaaggtgt acaatgctta
    72301 ttaacttatc atccaattac aagaggttta aatgattggc attttcttct catagtacct
    72361 gaagatgttg tacttggtga acttaataag ttaaattctt ttacaataat aatgacattt
    72421 ataactacat gtattattgg aattataaca ttaattttat atattataag aagaaaatat
    72481 gtaaatcaaa tagaaaatat tgcatattta gatagtataa caaatggtat aaattcaact
    72541 aagtttagta tgctagtcaa acctttaatt tcagaatcac ctgatagtac ttatatgatg
    72601 atagcaatga atataaaaga ttttaagcta ataaatgatt gtttcggtag tgaaaaagga
    72661 aatatgacac taaaacatct ttacaatatt ttgtataaaa acattgatga aaatgaagaa
    72721 tttgtgtgcc gtcatgatgc agaccttttc tatttgctta ttaagaatag accagtgtta
    72781 gaagccctgg agttattata taaaatagaa gctgacacaa attattttaa taaaaatagg
    72841 gaaaatccat actttttaag actatcagta ggtatttaca caattgaaaa tcataatgaa
    72901 gatttaatta caatacaaga ccatgcaaat acagcaagaa aaagctttaa taaaactcat
    72961 caaagtgatt tttcttttta tagtgatata gaaagaaaac gattaatatc tgaaaaagaa
    73021 atttcaaatt taatggaaaa agcccttgaa aataaagaat tttttatgtg cttacaacct
    73081 aaaattgata taaaaagtgg aaaaattagt ggagcagagt ctcttgtgcg ttggaaaaat
    73141 tcagaaaagg gcataattta tccaagtaat tttattcctc ttttcgaaaa aagtggattt
    73201 atatgtaagt tagatttata tatactagaa gaaacatgca aattgatttc taagtggata
    73261 aaagaaggta aagaagtgtt gagaatttct gttaatgtat cacgtcaaca tttgaatgat
    73321 aaattgtttt tagaaaaata taagcatata tgcaataaat acaatgtacc aacatgtctg
    73381 atagatttag aacttaccga atctattttc ttagaaaatc cagaagctat agatataata
    73441 gaggatattc attctaatgg atttaaatgt tctatagacg attttggatt tgggtactca
    73501 tcattaggga tattaaagga ttttaaagtt gatataatta agttagaccg ttcattcttt
    73561 gttagcaaaa ataatataga ccgtggtaag gtagttatta agtctattat tgaacttagc
    73621 aaaaggctag gtatgaaagt aactgctgaa ggaattgaag aattagaaca agttgagttc
    73681 ctaaaaaatg taggatgtga ttatattcaa ggttatgttt tttctaaacc attattaatt
    73741 tctgattttg aaaattttgc atatgaaaat aataaaatta aaaatatcca ttattaaatg
    73801 tacaagataa aaaatttagg caagttatat aattaaaaat ataacttgtc ttttttattt
    73861 atatatttaa taaaaatata tttgcagatt ttgtaaaaat aattttcaaa tgttgtaagt
    73921 tgtggctaaa gctttattgt attattctga atatagaaag atagttactg tcttagataa
    73981 aaactgaaac atacataata atataaaatt tgctatatta ataactctaa ttattttgga
    74041 aggagggaaa atatgataag tgggagaatg ttactcatca tgaattttct aaaaggaaaa
    74101 aataagacaa gttacaagga tattagcaaa caacttagta tagaagaaag aaaagttaga
    74161 tatgatataa ataatttaaa tattgttttg cgttcattaa acaagtcagt aatacaaaag
    74221 atgaacaaag gtgtgttaat tattcctgat gacttccaag tagtatgtat aaataataac
    74281 gaatatgtat tttccttaac tgagcgagta tctattatga aaattatatc aatgtttaaa
    74341 atagataatc taaacttaga aaaattgagt aaagaatttc aggtttctag aagttcaatt
    74401 aaaaatgacc ttaatgtttt gtcaagtgaa ttagaaagaa accaaataac tattacttat
    74461 gataaatctt ttaaaataag tggagaagaa gaccttatat ataagcaaag attgaacata
    74521 ttaaaatcgt attcttatct ttttggtaag gaaaaatctg atttaagtgt atttgagaag
    74581 tatttagtga atgtaattga aaaaatattc aaagggattt atttgattga tatatataat
    74641 tggtctatga atttattatt gcaaatggga tggactttaa atgatgaatc ttttacatgg
    74701 tatgtgtcaa acattttcct ttttacatgg tatatacatt cagatgaaaa acatccttta
    74761 gaatctgctt cattaagtga gccatgtttt gaaaataagt ttgaccaaga atttgaaaca
    74821 attattcaaa aaaagttgac taaggaacaa atttttctgt taaagagttt tgtatttttt
    74881 acaaataaat atgcaagttt aaacgaggat atggatttga ttagtacaga aattatagtt
    74941 caagacctaa tttacaatat gtcagaaata ttagagatta gctttaaaga agatatgatt
    75001 ctttataaag gattactcaa tcatattgcc ccattgatag agagaatcaa gcagaatata
    75061 cagatttatg aggaattact tcatgtaata ccagataaat atcaatatgt acttgaagct
    75121 actaaggttt ctattcaaaa aaacagagtt ttaagtcaaa taaagaatga aaatgaaata
    75181 atacttttgg caatacattt cttaggaagt atacatagaa atgagaggaa taactacaaa
    75241 aatatattgt tagtttgtgg atttggatat ggtgtaattg ctatgttaaa ggatagttta
    75301 gtaaatgatt accagataaa tatagtagag agtattccat catataagtt acaagattat
    75361 aaagaatggg aaagtattga cttcattata acaacatcaa agatagtaat agacttgcct
    75421 aaaacaatta ttgagataaa tccattttta caagaagagg attatttgaa gtttgaagaa
    75481 aaagggataa aaagaaagaa cgtcttaact aattatatgt caataaaaaa aaggttggac
    75541 tttttagaag aagatgctca aaaaagagta ttaagtatta tatgtcaaga acttggatac
    75601 agtgatgaga gaatattatt tagacaactt aatttaagtg atttaatagg gattgatacc
    75661 ataaggataa ttgatgaaga tatgaaatgg gaagatgctg taaaaattag ttcaaacata
    75721 cttgctaatt gtgattttgt atcagcacaa tatgctgaga atattataga tattttaaaa
    75781 aatgtgggtt tttatgctgt aaaagataac gaatttgctt tactacatgg caatgatagt
    75841 tcattagtca aagtaagttc gataagttta ttaatatgta aagaagcagt tagttttgga
    75901 gataaaaagg ttaaaatcat attttgtcta gcaagcagag ataaaaaaga gcaaattcct
    75961 gcaataatta atctaactaa aatggtttat aagactaatt ttattgctat gttagaaagt
    76021 gcaaagacaa aagaagaagc agaagtttta attcataaat atgagaagga ggtcactgta
    76081 tgaataaatt agttaatgta aaatgttcat ttaaaataca tgctaattca aaagaagaag
    76141 ctattgtatc gttagttgat gtagtatcaa aagaaggata tctaatagat aaaaatcaat
    76201 ttttggaaga tgttttaaag agagaagaaa cattgtcaac ttatattgga catggtatag
    76261 gtcttcctca tagtcaaagt gtaggtgtta aaaactcttg tattacaata ggaaagcttg
    76321 atattcctat tgaatggaca gaagaaggag aaaaagtgga tttaatattt ttaatatcag
    76381 taacaaaaga caatgaaaat aatcttcatt taaagatttt atcaaaatta gcacgtttat
    76441 tgatgcatga aagttttaga aatcaaatta gatgttcaga tgaacaaact gtttataatc
    76501 ttataaaaga aaaaatagag gaggaagatt aaaatgagaa ttttaaagtt tttgcaaaaa
    76561 catcttatga cagcaacatc ttatatgatt ccatttgtgg ttgcaggagg aatattattt
    76621 gctttatcag taactctaag tggacaagct gctgttcctg aaacaggctg gttggctaaa
    76681 ctgaatcaaa ttggagctgc tggcttggct ttattcattc ctatattagg tggatatata
    76741 gcatttagta tggcagataa accaggatta gctccaggta tgataggtgc atatttagca
    76801 aaagaagtaa atgcaggttt tataggtggt atcatagcag gatttatagc aggatttgtt
    76861 gttttacaat taaagaaaat taaattagct cctacaatga gaactttagg ttctatattt
    76921 atttatcctt tgattggtac attgattaca ggtggtataa ttgtattttt aattggagaa
    76981 cctatagctt cttttatgac atggatgaca aactggttaa atggaatgtc tggtgtttct
    77041 aaaattcctt taggtggaat cttaggagga atgatagcct cagatatggg tggtcctata
    77101 aataaggttg ctgctacttt tgcccaaact caagttgaca cacttccata tttaatgggt
    77161 ggagttggcg ttgcaatttg tatacctcca attggtttag gattagctac attattgttt
    77221 ccaaaaaaat ttagcaagga agaaagagat tcaggtaaag cttcgctatt aatgggatgt
    77281 gttggaatta cagaaggagc tattccattt gcaacatcag atccagttag agtaatacct
    77341 tgtattatgg ctgggtcgat tataggaaat attatggctt ttttattagg atgtttaaat
    77401 catgcaccct ggggaggtct gatagtatta cctgtagttg acaataggtt ggggtatatt
    77461 gcatcagtat taactggagc agtagttgtt gcagtattaa tgaaattagt aaaaaaagac
    77521 gtgaaagaag atgaagaaat tgaagaggat ttagatgaat caattgaatt aatatttgaa
    77581 gaattataaa tataaatgga aggaatttgt ataaaaaagt aaatataaaa ctataaactt
    77641 aaaggagata agaatatgaa aattgtagca gtaacatctt gtcctagtgg agtagcacat
    77701 acttacatgt cggctgaatc acttgaatta tcggccaaaa aatttggtat agaaataaag
    77761 gttgaaactc aaggaagctc tggaatagat aatgaattgt cacttaaaga tattgatgaa
    77821 gcaacttgtg taatattgac taatgatgtt gagattagaa atatggaacg ttttaaggga
    77881 aagaaggtag taagaatgag tgtatctgag ataattaaga aatctgatgc attaattaag
    77941 aaaataaaag atacttttca ataaaaattg atttatttta catataactc aaagatgttt
    78001 ttaaagaaat ttttattaaa agcatctttg aaaatatctt aaaaatagaa taaaaggaga
    78061 tatagtcatg aaaaaattac caataaagac tgtcgtacaa agattactag acttacaaga
    78121 agaggggaaa agtgcaactc ttttaggaat tggtcctatg tcaccaaact tgttacaagc
    78181 aagttttgaa ctagcaaaag atgatgattt tcctttaatg tttatcgcaa gtagaaatca
    78241 agtagatgca gatgaactag gtggcggtta tgtaaatggt tggaatcagg aaacttttac
    78301 aaaagatatt aagaagattg ccgacaaagt tggatttgat ggtttatatt atttatgtag
    78361 agaccatggt gggccatggc aaagagataa agagagaaat gaccatttgt ctgttgatga
    78421 agcaatggag ttaggaaaaa aatcttattt agcggacatt gaagcaggtt ttgacctatt
    78481 gatgattgac cctacaaaag acccatttga aattggaaaa gtaattccac tagatgtagt
    78541 actagaaaga actgtagaat taattgagta ttgtgaaaac gagagaaaaa gacttaatct
    78601 accagacata ggttatgagg ttggtacaga ggaaaccaat ggaggtctta cttcaactga
    78661 gacgtatgaa acatttataa ctagactaaa ggttgaactt gataataggg gtttgccaat
    78721 gccaacattt atagttggtc aaactggtac tttaactaga aagacagaac aagtaggaac
    78781 ttttaatttt agaaatgctt atgatttagc tcaaatggct aagaaatatg gagttggttt
    78841 aaaagagcat aatggcgatt acttagatga tgtaacttta ttagaacata taccatctca
    78901 aattattgct acaaatgtag ctcctcaata tggtactgaa gaaactaggg catatttaaa
    78961 attagcagaa gttgagtgta aattagaaaa agagggccta gttgataaaa cttctaatat
    79021 cagacatgtt cttcttgtta atgcaattga atgtggacgt tggagaaaat gggtagtagg
    79081 agaacaaaaa aatctaacta ctgaagagat atttaagaat gaagttttat ctaatgaaat
    79141 actagatatt gcaggacatt atacatttaa taatgatgat gttaaaaaag aaatagaagt
    79201 gctttatgat aatttaagta agaataatat tgatggtcaa agatttgttg tagaccatat
    79261 taaacgtcct ttgcgtgatt atgcagaatg ctataactta aaaggtgtta ctacgaggat
    79321 tttaaataaa taataggact tatagtggtg agtattgtta tactaggtat ttatagttat
    79381 aattatactt accactatta aatttaggag gactaatatt ttgattaatt taatttttga
    79441 tgtagatgat actttatata accaattaac gcctttttac actgcatata ataaagtgtt
    79501 ttcttcaata aaggatattt ctattgaaga tttatatatg agtagtagaa agtatagtga
    79561 tgaggtattt catatgactg aaaatggaga aatgccaatc aaagaaatgc atatttatag
    79621 gataatgaaa gcttttgagg agttgggaaa ttctattaca gaaaaagatg cacaaagttt
    79681 tcaagatgag tatatatatc agcaatcaca aattacacta ataccagaag ttgagaggat
    79741 tttaaatttt tctaaagaaa gaaatattaa tctaggaatt ataactaatg gcccatcaaa
    79801 ccatcaaaga atgaagttaa agcagttaaa tattgaaaat tgggttgata aaagtaatat
    79861 ttttatttct agtgaggttg gattttctaa gccagatact aatattttta gagtagctga
    79921 gaatgttatg aatttagata gagaaaatac atattatgtg ggtgattctt atagaaatga
    79981 tgtattaggt gctaaaaaag ctggttggaa gagtatatgg ttgaaccata gaggacatga
    80041 ggtagaagag ttgttttata aacctgattt tgtaattttg gaacataagg atttgatatc
    80101 tttatttata aaaatatgta gttataaaaa tatgtagtaa aaaaatgtta ataatagaat
    80161 tgtataaaat aatta
//
LOCUS       DAELSY010000002        68365 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.002, whole genome shotgun sequence.
ACCESSION   DAELSY010000002 DAELSY010000000
VERSION     DAELSY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 68365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68365
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.002"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            219..1436
                     /locus_tag="KPG09_000111"
     CDS             219..1436
                     /locus_tag="KPG09_000111"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBG0964741.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1531..1911
                     /locus_tag="KPG09_000112"
     CDS             1531..1911
                     /locus_tag="KPG09_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG0964742.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2195..2677
                     /locus_tag="KPG09_000113"
     CDS             2195..2677
                     /locus_tag="KPG09_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0964743.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVNLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2698..3513
                     /locus_tag="KPG09_000114"
     CDS             2698..3513
                     /locus_tag="KPG09_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0964744.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3564..5114
                     /locus_tag="KPG09_000115"
     CDS             3564..5114
                     /locus_tag="KPG09_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0964745.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5211..6563
                     /locus_tag="KPG09_000116"
     CDS             5211..6563
                     /locus_tag="KPG09_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG0964746.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAESLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKVFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6694..7452
                     /locus_tag="KPG09_000117"
     CDS             6694..7452
                     /locus_tag="KPG09_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0964747.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIYNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7499..8218
                     /locus_tag="KPG09_000118"
     CDS             7499..8218
                     /locus_tag="KPG09_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG0964748.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8342..9742)
                     /locus_tag="KPG09_000119"
     CDS             complement(8342..9742)
                     /locus_tag="KPG09_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBG0964749.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9746..10654)
                     /locus_tag="KPG09_000120"
     CDS             complement(9746..10654)
                     /locus_tag="KPG09_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0964750.1"
                     /translation="MKGDNKVTEKQRLIINIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10915..11562
                     /locus_tag="KPG09_000121"
     CDS             10915..11562
                     /locus_tag="KPG09_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0964751.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11887..14526
                     /locus_tag="KPG09_000122"
     CDS             11887..14526
                     /locus_tag="KPG09_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG0964752.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14584..15687)
                     /locus_tag="KPG09_000123"
     CDS             complement(14584..15687)
                     /locus_tag="KPG09_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBG0964753.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSSK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            16048..16869
                     /locus_tag="KPG09_000124"
     CDS             16048..16869
                     /locus_tag="KPG09_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0964754.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16948..17159
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17246..18562
                     /gene="argH"
                     /locus_tag="KPG09_000125"
     CDS             17246..18562
                     /gene="argH"
                     /locus_tag="KPG09_000125"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBG0964755.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            18811..19149
                     /locus_tag="KPG09_000126"
     CDS             18811..19149
                     /locus_tag="KPG09_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBG0964756.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(19276..20565)
                     /locus_tag="KPG09_000127"
     CDS             complement(19276..20565)
                     /locus_tag="KPG09_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG0964757.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFFTGNNILMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            20645..21349
                     /locus_tag="KPG09_000128"
     CDS             20645..21349
                     /locus_tag="KPG09_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HBG0964758.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            21441..22487
                     /gene="selD"
                     /locus_tag="KPG09_000129"
     CDS             21441..22487
                     /gene="selD"
                     /locus_tag="KPG09_000129"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:21489..21491,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBG0964759.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDNPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            22505..23917
                     /locus_tag="KPG09_000130"
     CDS             22505..23917
                     /locus_tag="KPG09_000130"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBG0964760.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVAELRKAFN"
     gene            24031..25914
                     /gene="selB"
                     /locus_tag="KPG09_000131"
     CDS             24031..25914
                     /gene="selB"
                     /locus_tag="KPG09_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBG0964761.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALEALISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            26019..28757
                     /locus_tag="KPG09_000132"
     CDS             26019..28757
                     /locus_tag="KPG09_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBG0964762.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSIILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(28876..29820)
                     /gene="manA"
                     /locus_tag="KPG09_000133"
     CDS             complement(28876..29820)
                     /gene="manA"
                     /locus_tag="KPG09_000133"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBG0964763.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            29993..31354
                     /locus_tag="KPG09_000134"
     CDS             29993..31354
                     /locus_tag="KPG09_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0964764.1"
                     /translation="MELFKAESKKYEKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSIVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            31442..33331
                     /locus_tag="KPG09_000135"
     CDS             31442..33331
                     /locus_tag="KPG09_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0964765.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIYCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            33346..33801
                     /locus_tag="KPG09_000136"
     CDS             33346..33801
                     /locus_tag="KPG09_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0964766.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            33860..34177
                     /locus_tag="KPG09_000137"
     CDS             33860..34177
                     /locus_tag="KPG09_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG0964767.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            34202..35464
                     /locus_tag="KPG09_000138"
     CDS             34202..35464
                     /locus_tag="KPG09_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG0964768.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFIDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            35569..36633
                     /locus_tag="KPG09_000139"
     CDS             35569..36633
                     /locus_tag="KPG09_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0964769.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKIGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKIIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            36687..37865
                     /locus_tag="KPG09_000140"
     CDS             36687..37865
                     /locus_tag="KPG09_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBG0964770.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFVPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKNIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(38056..39114)
                     /locus_tag="KPG09_000141"
     CDS             complement(38056..39114)
                     /locus_tag="KPG09_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBG0964771.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTIFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLEDGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINEIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            39181..39552
                     /locus_tag="KPG09_000142"
     CDS             39181..39552
                     /locus_tag="KPG09_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBG0964772.1"
                     /translation="MKKKDILLILGILVVIIACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            39589..40263
                     /locus_tag="KPG09_000143"
     CDS             39589..40263
                     /locus_tag="KPG09_000143"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBG0964773.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            40512..41351
                     /locus_tag="KPG09_000144"
     CDS             40512..41351
                     /locus_tag="KPG09_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG0964774.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            41594..41690
                     /locus_tag="KPG09_000145"
     tRNA            41594..41690
                     /locus_tag="KPG09_000145"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41628..41630,aa:Sec,seq:tca)
     gene            41841..42548
                     /locus_tag="KPG09_000146"
     CDS             41841..42548
                     /locus_tag="KPG09_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBG0964775.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(42690..43526)
                     /locus_tag="KPG09_000147"
     CDS             complement(42690..43526)
                     /locus_tag="KPG09_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0964776.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHIIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNVIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            43714..44619
                     /locus_tag="KPG09_000148"
     CDS             43714..44619
                     /locus_tag="KPG09_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBG0964777.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            44628..45812
                     /locus_tag="KPG09_000149"
     CDS             44628..45812
                     /locus_tag="KPG09_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0964778.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     RIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNTYINE
                     YI"
     gene            45927..47228
                     /locus_tag="KPG09_000150"
     CDS             45927..47228
                     /locus_tag="KPG09_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBG0964779.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTLIESIFFLLEKISNLSFAYINIESPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            47182..48225
                     /gene="holA"
                     /locus_tag="KPG09_000151"
     CDS             47182..48225
                     /gene="holA"
                     /locus_tag="KPG09_000151"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBG0964780.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(48412..48678)
                     /gene="rpsT"
                     /locus_tag="KPG09_000152"
     CDS             complement(48412..48678)
                     /gene="rpsT"
                     /locus_tag="KPG09_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBG0964781.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            49277..49798
                     /locus_tag="KPG09_000153"
     CDS             49277..49798
                     /locus_tag="KPG09_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0964782.1"
                     /translation="MDIAKLILNPARLRILQYIRLHGSARTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVVDNDKTK"
     gene            49832..50608
                     /locus_tag="KPG09_000154"
     CDS             49832..50608
                     /locus_tag="KPG09_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0964783.1"
                     /translation="MKFHEFGDKNKPHIMLIHGGGNAWWNYLRQAEVLSNNYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKM
                     FQSYVPSCEIYEAKGYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            50951..51904
                     /locus_tag="KPG09_000155"
     CDS             50951..51904
                     /locus_tag="KPG09_000155"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBG0964784.1"
                     /translation="MINVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            51922..52941
                     /locus_tag="KPG09_000156"
     CDS             51922..52941
                     /locus_tag="KPG09_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBG0964785.1"
                     /translation="MFKKCIKVVTLTFILACILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEENTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            52972..53223
                     /locus_tag="KPG09_000157"
     CDS             52972..53223
                     /locus_tag="KPG09_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964786.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIIGTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            53505..55310
                     /gene="lepA"
                     /locus_tag="KPG09_000158"
     CDS             53505..55310
                     /gene="lepA"
                     /locus_tag="KPG09_000158"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBG0964787.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            55461..56798
                     /locus_tag="KPG09_000159"
     CDS             55461..56798
                     /locus_tag="KPG09_000159"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBG0964788.1"
                     /translation="MINIEIPKKVDYIIKELETNGYEAYVVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLISTEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILRRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGHILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            57031..58344
                     /locus_tag="KPG09_000160"
     CDS             57031..58344
                     /locus_tag="KPG09_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0964789.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTIISLGGIFITLLGLPVYYYMKKR"
     gene            58516..59691
                     /locus_tag="KPG09_000161"
     CDS             58516..59691
                     /locus_tag="KPG09_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBG0964790.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMKLY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINVDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            59794..60813
                     /gene="hrcA"
                     /locus_tag="KPG09_000162"
     CDS             59794..60813
                     /gene="hrcA"
                     /locus_tag="KPG09_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBG0964791.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            60843..61463
                     /gene="grpE"
                     /locus_tag="KPG09_000163"
     CDS             60843..61463
                     /gene="grpE"
                     /locus_tag="KPG09_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBG0964792.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            61555..63402
                     /gene="dnaK"
                     /locus_tag="KPG09_000164"
     CDS             61555..63402
                     /gene="dnaK"
                     /locus_tag="KPG09_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBG0964793.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLADEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            63613..64767
                     /gene="dnaJ"
                     /locus_tag="KPG09_000165"
     CDS             63613..64767
                     /gene="dnaJ"
                     /locus_tag="KPG09_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBG0964794.1"
                     /translation="MSTKRDYYEVLGISKDAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            65733..66668
                     /locus_tag="KPG09_000166"
     CDS             65733..66668
                     /locus_tag="KPG09_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG0964795.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     AEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKDYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            66704..68077
                     /locus_tag="KPG09_000167"
     CDS             66704..68077
                     /locus_tag="KPG09_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0964796.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEGNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
BASE COUNT        26905 a         7419 c        11883 g        22158 t
ORIGIN      
        1 tttaataaga atgtaaaaaa tatttttaaa ttatcattta atgtaattta aatgaattga
       61 agatatatgt ttattgtatg caattgttgt atttttaggt tttttgtgat taattaaaaa
      121 attataaaaa tcgtttacat gttgattttt aaaaaaaaaa gatatagaat tatatagaaa
      181 gtaataaaca aaaattggat ttacgggagt ggaatgaaat gatgaatgtt accttaacag
      241 atgtgaaaga ggctagagag accattaaga acatagtaaa aagaactgac ttacttgaaa
      301 gtgttaaact tagcgaaaaa acaggagcaa atgtgttcta taaatgtgag aatcttcaaa
      361 aaacaggttc ttttaaatta agaggagctt gtaacaaaat agcgagttta acagatgaag
      421 aaaaggcaaa tggagttata gcttcaagtg cagggaatca tgctcaagga gttgcattgg
      481 gagcaaaaat gactggtata aaatctacaa tagtaatgcc agcaactgcg cctttagcaa
      541 aagttagtgc aactaaaggt tatggagcag aagtagtttt aaatggtgcg gtttatgatg
      601 atgcatatgc aaaagctgtt gaaatacaaa aagaaacagg agcaactttc ttacatccat
      661 tcaatgataa gtatgttata gcaggacaag gaactatatc tcttgaaatt ttcgaacaat
      721 tagacaataa agttgatact atactatgcc cagttggtgg aggaggaatc atctctggtg
      781 ttgctgttgc agcaaaagca ttaaatccta atgtaaaaat agtcggtgtt caaactgcaa
      841 atataccttc aatgaaagag tcaataaaaa atggaaaagt aacaacagca tttaatgata
      901 ctacaatagc tgatggtata gctgttaaaa ctccaggaga tttaactttt gaaataataa
      961 atgaattggt tgatgaaata gtagtggttg aagagacaga aattgcagaa tcaatattat
     1021 ttatgatgga aagtcaaaaa atagtttcag aaggtgctgg tgctgtatgt acagctgcta
     1081 tattaagtgg taagtatgta ccagcaaaag atgaaaatgt agtttgtata atatctggtg
     1141 gtaatataga cataaatacg ttatatagaa taataggtgt tgctttagct aaagaaggaa
     1201 gaagatattc atttagtaca attatggaag ataagccagg aaactttgct gagttaacta
     1261 gaataataag tgagaatggt ggaaacattt taagtgctaa tcaaggtaaa ttatcagcag
     1321 gggaagctct aggaaaacaa agtgcggaat ttatattaga gacaatagat tatgaccata
     1381 tagctagaat aaagaaagct atagaagaaa aaggttttaa aattatagaa ttataataaa
     1441 aagtgtatat aatatacaag aaaaatagta taatagtgta tgaagataca ttttatgtgt
     1501 attagattaa cgaattagga ggattttaaa atgaaacatg aagtaataca tactaatgat
     1561 gcaccagcag ctttaggacc atattctcaa gctataaaag ctggaaactt attatttgta
     1621 tctggacaag ttcctcttgt accagagact atggaagtag ttgaaggaga tgtacaagct
     1681 caaactgctc aatctttaaa aaatcttaaa gctatattag ctgaaagtgg agctgatttt
     1741 tcaaatgtag ttaagactac agtatttata aaagatatga atgaatttgg ggctataaac
     1801 gaagtttatg ctgaatattt tggagaaaat aaaccagcaa gagcttgtgt agaagttgct
     1861 agattaccaa aagacgttaa agttgaaata gaagtaatag ctgtattata aaaatacaat
     1921 atttttaatt tgaaaaggct gaaaaactat attctagtga ttcagtcttt ttataaaaaa
     1981 ataaatcaca ataaagaaca tgataaatat tgtcgaatta aaaaataaaa ttaaaataaa
     2041 tattgcaata tttttgagaa ggttttataa tgagtatata atttgaaacg tgtaactatt
     2101 aaattaggca tgagttcttg gatattttta tcctgaggat tcatgccttt tttgtattga
     2161 aattcaaaaa tctatttaat aaggaggaag tattgtgtta aattttttca agaaaaataa
     2221 aagctataag ttacatgcag tagtgagtgg taacagcatt aatattgaga aagtaaacga
     2281 ttctgtgttt tcaaaaaagt taatgggaga tggagttgca attattccta atagtaatgt
     2341 tgttgtagcc ccttgtaatg gtaaagtaac tgtgcttaca gaatcaaagc atgcctttgg
     2401 tatggtctca gatgagggtg ttgaaatatt agtccacata ggaattgata cagttaattt
     2461 acagggtgag gggtttaaaa atgaagtgtc tcaaggagat actgttaaaa aaggtagtcc
     2521 aataataagt tttgaaagag aaaaaataaa ttctcaggga attgattgta caacaataat
     2581 aattgtatta aatcatagcg aattttcaga aataaattgc atggtagaaa atgaagttgt
     2641 tgcagggcaa gatactgtta ttgaaattat gaaataatag gaagggatta attcaatatg
     2701 agagtagtaa aaatctttaa taataatgca ttatctacag taactaatga taggaaagaa
     2761 gctattttac taggtcttgg tataggtttt aataaacgtc caggagataa agttaatgaa
     2821 gataaaatag aaaagattta ttatgttcaa gaccacatgc aaacaaaatt tttagaatta
     2881 ttaaaaaatg ttagtccaga agtgatggat gcttcacaac aaattataag tcttatacca
     2941 ggaaatgagg gaaaatttaa taataaaggg atattatctt tagtggaaca tattagtttt
     3001 gcaatagata gaatgaaaaa taatgttttt ttacctaatt taatgctttt ggatataaag
     3061 atgatgtatt caaaagagtt tgaattagga gtaaaagctt tagaaattat atatagagta
     3121 tgtcatatcc atttgcctga agatgaagca ggttacatag ctttacattt tgtcaattta
     3181 caatcaaatg ataatttggc atatgatact ttaaaatttg taaaaggtag tattgatttg
     3241 attaaagaat gttatggatt agaattagac gaatcgagtt taagtacatt acgttttaga
     3301 actcatctta aatttcttgc tcaaagaatt tttaagaatg aaatttgtca ggatgataaa
     3361 atgattgaaa tgtatgatta tttaattaac aatcatccta aaaataaaga atatttagaa
     3421 aagttaaatg tatatattga aaaggagttt aggtataaat tagataaacc tgaaaaaatt
     3481 tatctactac tacacctaac aaaaatctta taagaatgag atgaattagt aatagaaata
     3541 tataatttta gggggggata agattgaaag aaaaactact tggaggactg gaaacatttg
     3601 caaaggcaat ggttcagcca ttaatgtatt tatcgagtgc aggaatatta atggtggtag
     3661 gtgtactact tacaaatgat atgttattag gagtacttcc attttttaaa tgggagccaa
     3721 ttcaaatatt tggaagccta atttataact gtataatggc tattattaat aatcttgctg
     3781 ttatatttgc tataggtatt cctgctgcac tagcaaaaaa agagaagcac aaagcagcgt
     3841 taataggatt tatgagttat ataatatacc ttacagcttc caacactatg ttaacatcac
     3901 taggtcaact agcaaaagaa agcaagatgc ttggactgat agggactgga caagctacta
     3961 ttttaggtat tcaagttgta gatacaagtg tatttggtgg aattatttta gggtgtatag
     4021 ctggatatgt atttaatcgt acaagcaaca aaacatttaa aggagcttta cagatatact
     4081 cgggagcaaa ttttagtttt atatgcatgt tctttgtatc cattttaatt ggagttgtga
     4141 gtaactttat atggccatta gctcaacaag gaatagcttc tcttgcaaat gtcatcaaag
     4201 atacaggtac atttggttta ttcttatatg gttttttaga gagaatatta gtaccaactg
     4261 gacttcatca tttaatctat acaccatttc agttttctga tttaggagga gtgcttaaag
     4321 ttggtgcaga tacagtttca ggagcctatg ctatagttat gacagaaatg aatttaccag
     4381 ttgagcgttt ctcagatagt atatactaca tggcaactgg atttacaaag atgtttggtt
     4441 atataggtat aggagctgcc tttatttata ctgcatataa gcaaaataag aagaaagtta
     4501 aggcaacgat tattccacta ataattacgg cagtattagc ctctgtaaca gaaccattag
     4561 actttatgtt tgtattctgt gcaccacttt tattctttgt acatgcagtt atagctggtc
     4621 tttttatagc tttattaaaa atatttgatg ttactgcatt atgtggaggg aacttattag
     4681 tatcaattat aatgaatact acagtaggtg tagaaaaaac acactggcca gtaatgatga
     4741 tattaggact tgcacaaatt attgtatatt ttgtagtatt tagtttttta attaagaaat
     4801 ttaattataa aactccaggt cgtgaagatg agagtacagc tacaggcgaa gaacaatcaa
     4861 aacaacttaa tttagatgca ggtatagaaa atataattga tgggttaggc ggaaaagaaa
     4921 atattaatac agtagaaaat tgcatcacta gattacgtgt taatgtaaaa gatgaaagta
     4981 agattaatga agatataatt aatttaacgc caaatagcgg gattgtaagg aaaggaaaag
     5041 atatacaaat aatttttgga cttcatgtgc atgaggtaag acgtgcagta gaagattttt
     5101 tagaagctta ttaaagatat atatttatat agcaagggta aataacagct ttaagtgctg
     5161 tttactattt attaaagata catataattt atagaggagg aataattatt atgattatta
     5221 ctattgttgg tggaggaagc acatttactc caggtattgt taagtcaatt gcgttaagag
     5281 aaaaagagtt agagcttgag gaaattcgct tatttgacat tgataaagaa cgccaagata
     5341 aagtagctgt agttgtcaac tggatactaa aagaagatgt taaaacagat gttaaactaa
     5401 ctgtaactaa ttgtgaaaaa gaagcgtata cagatgctac atttatattt gcacaaatgc
     5461 gtgttggtaa atatgaaatg cgtgagcagg atgaaaaaat tccattgaaa tatggttgtg
     5521 taggtcaaga aacttgtggt tgtggaggga tggcatacgg actgcgtaca atacaaccaa
     5581 tgataaaggt tattgatgat gtagagaaat atgctaaaaa agattattgg atattaaatt
     5641 actctaatcc tgctgcaatc gtttctgaag cttgtcgtaa gttaagacca aatgcaagga
     5701 ttataaatat ctgtgatatg ccaatagcta ttatagatat gatagcggag tcattaaata
     5761 tcaatgacgt tcataatata cgctatgatt attttggtct aaatcatttt ggctggttta
     5821 cttcaataga ttataaacat agagacttaa tggaagagat taaggaatac ataaaagaaa
     5881 ataaaatatt attaccaaaa tcttatatag aaaacttaaa taaaagtcgt agtacaggaa
     5941 atcgccatgc tgtaagtagc tggatacatg tttgggaatc tgtttataca atgttagaaa
     6001 gcttcccaaa atatctgcca aacacttatt taacatacta tcttttaagt aaagaaacag
     6061 tggaacattc aaacataaat cgtactcgtg caaatgaggt tatggaaagc cgtgaaaaag
     6121 acttatttga aggtgtgaga cattacttag aaactggaga aatttctgaa aaagtatttt
     6181 atgcaggttc acatggagat tggattagtg atttggcagt ttcaattaaa aatgatacac
     6241 gtagtcgttt tttagttata actgaaaatc gtggagctat accaaacatg ccatatgatg
     6301 caatggtaga aattccagca tatattggta aagatggtcc agaagttatt gctcgaggtc
     6361 caatacctct attccaacaa ggattaatga tgcaacaact taacagtgaa aaattgatag
     6421 tagaagctta tatagaaaat agttatgaaa aagctttaca agcattcaca ttaaataaaa
     6481 ctattccaag tatgaatgtt gctaaagaaa ttttggatga aatgatagaa gttaataagg
     6541 aatactggcc agaattaaga tagtaggata aaagaaaggg tttttattta ataattagat
     6601 atgttttcag agtcttttat caaaatgtat tcacaaacat atggcaatat attaaaataa
     6661 agagtgatat ttttagaaga atggggtgat gcaatgaaca ttataaataa aattttttgg
     6721 caagaagata ggattggcat gattacaaat aatttagagg cagatacaca tagccactgc
     6781 atgttacaac tgttcttagg gatagaagac agcattgaaa taacagtcaa tgaaaaatta
     6841 gttaaatgta attgtatcat agtagataaa aatatctctc attcattttc agcaagaaaa
     6901 aaagtttatt actctgctat tattgaacct acatctatat atgcagaaca gcttacctca
     6961 aaaatgaatg attttggcta ctggatatgt gacaatgatg gattagagaa attacgtcaa
     7021 caaggaactt ttttgatata taactctagt aaagaacaat atttaagatt tatggaaatg
     7081 cttaataact atttgaatat acctataact ctcaaacatt atgatgacag aattactgaa
     7141 ttgctcaatt tattacgtac ttgtaattgt gataatcata ctatttcaag ttttgcagat
     7201 aaagtgtccc tatcagcaag tagattatca catctgttta aagaacaaat agggatacct
     7261 ttaaaaagct atatcttatt tcatcaaatg gagtgtgctt ttagagaatt actttctggg
     7321 aaaaatgtta ccgaagcttc tatgactgct ggattcgata ctccatcaca ctttgcaggg
     7381 acagttaagc gaatgatggg aatgcctgtt tctttatctt tgaaagatag cgaatttttg
     7441 aaagtatatt aaatattctc gttatataat gaagttgtac taagaaaggg gctttgttat
     7501 gaataaagaa tatttagagg aaacaaaaat gttaaattat aatgaaccac aacttaagct
     7561 tttggtatca tcaaagaatt ggttggaatt agatgatttt cataaaataa aatctattta
     7621 tgagtttgta cagaatgata ttttatttgg atataatgct tctgatatgt taagcgcaac
     7681 acaggtatta aatgatggta tgggacaatg caatacaaaa gctactcttt taatggcatt
     7741 attacgtgca gtgaatatcc cttgtaggct tcatgctttt gatgtaacaa aagattttca
     7801 acgaggtgct acatccaaat tgatttcact attagcacca aaatatattc ttcatacttg
     7861 ggtagaggta ttttatcaag ataggtggat tgctctggag ggtgtcataa ctgataaaaa
     7921 atatttagaa gcaattcaga agaagttttt taatcatggt ggaacgttta aaaagtatgc
     7981 aatagctaca aatgatttga aaaatacatc tattgattgg gatggtaaag atacttttat
     8041 ccaaaaagaa gctatcgtgt atgattatgg tatttttcca tcaccagatg tatttttttc
     8101 tactcattca caacatatgt caaaattaaa aaatttcata tatgtacatc ttatcagaaa
     8161 aataatgaca aaaaatgtat gtaaggctag aaacaattat attgataaaa acgaatgagt
     8221 gtttaagagg taactatata ggaaatatat agaataatta aaactgtact ggttaaaatg
     8281 aataaaaagg agttatcata gagcaaactt aaattgttca taggataact ccttttctgt
     8341 attaacttct aataatttga tgtcttttta taaaacatat agatacaatt aaaccagtaa
     8401 atgcaagagt agctgctatt gaaaatgcaa catctattcc atgaatgtta gacagcatgt
     8461 ttgaactcat atgagctttc ttagtagcac ttgtcattat agtaataagt atagcagaac
     8521 ctatagctcc agatatctga cggagtgtgt tattaattgc tgtggcatga gatataagtt
     8581 ctctatctaa tgttttaatt ccccatgtag ttaatggcat taaaaccatt gaaataccaa
     8641 tcatacgaat acaatacatc aatgacacat gaattaaaga tgtatctctg cctaaaaaag
     8701 cgaaggataa agttcctaaa aacaaacatc ctgttcctaa tattgataga gcatgaggtc
     8761 catatttatc taaatgacgt cctgcaattg gattgagtac aaccatcaat atagcaccag
     8821 gtaacattaa tgaacctgaa gataatgcag aaaatcctct tacagattga acgtacagag
     8881 gaatcattaa tgtagcagac atcattgagg catatacaat cataattaat attgtagaaa
     8941 taagaaatgg tttattttta aaaaccctca attctaaaaa aggttcttta gattttaatt
     9001 gacgcaatgt aaataaaatt aatgacataa ttcctattaa tattggtaaa tatgtggcaa
     9061 tattagtcca cccataatta ccttgattag taaatccaat taataaacca ccaaaaccta
     9121 gagatgatag tactattgaa ggtatatcta actttagttt ttgagtttcc ccaacatttc
     9181 ttaatagaat aaatgatagt ataacatcta atatagcaat aggggaaaga aacagaaata
     9241 aactatgcca accaaatgaa tctactaacc atcctgataa agttggacct attgctggag
     9301 caaatcctac tgtaatacca actaaactca tagctgcacc acgtttttcc tcagggcata
     9361 aatagagtat aataacttgt aaaagtggca ttagagaacc agaacccatt gcctgcaaca
     9421 cacgagcaat caacatagtt gaaaaattat tagaaaaaag tgcaataata catcctatag
     9481 aaaagataga cattgatgta ataaataact ttctagtact aaatcgattt attaaataag
     9541 ctgttgtggg tacaataata cctgcaataa gtagataaat tgttgttaac cactgaccaa
     9601 tatcagcagt aattttaaaa tctgacatta tattaggtaa tgctgttgac aatagtgttt
     9661 gactgagaga acttataaaa gcaccagtca ttacaattgc tattattaaa tttccttttt
     9721 tattgttact ttgggtttcc ataatttata acgtctcctt ttttaattga tttttaatgc
     9781 tgttcaataa aagtactttt ggtcttaaag tgtcttcttt ttctaaatat gcaatcattg
     9841 catccataat taaagaatca tattgttgat tttttccctc gacgtataat ttttttattg
     9901 cttctagtaa tttaggattt tcaaggagag aagtatcagt tttgattagt tcttctatat
     9961 cttgtatcgt tttaaaaaat agcgatttat tatgaaggga aaagtaaact tcatcttctt
    10021 gtttatgtaa aaacattgat ttttcaatta aaggatttag attatccata ttttctacaa
    10081 ctaaatctag aatttgtata gtaaatttgg caataaagta tacatttatt tctctttttt
    10141 gtacatgaat aatgtatata aactgatgaa ttagagcatc caataagaaa actaaatcaa
    10201 atataatttt ttctatttta tcaccatagt tatcaaataa gcttaatttg tattcattca
    10261 ataagttagc tcgtgatttt gtttgcaatt tacttgtagc cttacgttgc tcttcagaaa
    10321 aattttctct tatataggta tttatggtag ttctagaaaa aatatttttc catacagcaa
    10381 ttatctttct gtttaacttt tctgatgatg gaatgtcatt attttcgttt atggaatgta
    10441 taatttctaa aaacttttca tcataataat tgattatttc gcagataaaa ctttccttag
    10501 tttcaaaatg cttgtaaaaa gtagctttag acattttaca ctcttttaca acatccgata
    10561 ttgaagtatt ttgaaaacct ttttggtcaa aaattttttg tgcaatattt ataatgagcc
    10621 tttgtttttc tgtcacttta ttatctcctt tcacgcaaaa acgtttagta ctatatagta
    10681 cctacaagtt ttaataaata tattatacat catttggaaa aaaaatcaat atatagactg
    10741 taatatgaaa tttttgaaaa atttgttgtt atttttagtc gtataaaaaa gaaacttaag
    10801 agtttcttaa gaattgttta aagaagttgc aataaattag tggtatgatt atagtatgaa
    10861 agagaatgtc ttgaaatttt tctgtatcaa attccaatta tggaggtgta gtatatgaaa
    10921 aagaaaattt gtgtagcaat aatttttgct ctatttatag tttttgtaga aatgtttatt
    10981 gatagctcta gcgaaggaag taaaattatg acagctgaat taagtaaata caggaaaggc
    11041 gtaaatgtat tatcaataaa caatattata gtagcgaata aaaaatatag tttgcccaaa
    11101 gattattctc ctcaggaaag cagtgaagcg agagatgctt tttataaaat gaataaagat
    11161 gctcaaaagt caggattaaa tttaaaagct tttagtacat atagaagtta tgaatatcaa
    11221 gatagattat ttaaatcata tgtaaaagaa catggagaaa aagaagcaaa tagattttca
    11281 gctaaacctg gagagagtga gcatcaaact ggacttgctt ttgatattgg aggagatgac
    11341 cagtcttgtt gggcaaataa aaagttcaac aatacaaaag aagctaagtg gttgtatgaa
    11401 aatgcatata aatatggttt tatacttaga tatccagaag gtaaagagaa tataacagga
    11461 tatatgtatg aatcttggca ctatagatat gttggaactg aacatagcaa aaactttgca
    11521 atgaataatt tgactttaga agaatattta catataaatt aagagatggt ttgttaaaat
    11581 taggttaaaa taaaaaataa cctagactat tagctgtgta aagtgatata atgtaaatac
    11641 aatttaagat aactactcga aggggagtag cttacacgaa agtggtaatt aatcgtcaag
    11701 acagtagaaa tactcggtta attagcaagg gattccttga tagcaagacc ttcaaaataa
    11761 gcattaggta tgtttgtttt ggggtctttt tttattcaag gaatttttat ttaaaaaaat
    11821 ttaaatttca agtatacata agagccccca aacataaaat aaatttattg aagaggaggg
    11881 caacaaatgt cttacaaaag tacttatcaa gaagtaatta aaaatttaga ctcaagttta
    11941 tctggtttga gtaaagaaag agcagaagag ttgttggaaa aaaatggagc taatgaatta
    12001 aaagaagcag ataaggttcc tacttataag cttttcctag aaagttttaa agacccatta
    12061 gtaataattc tattgatagc tgctttagta caaatatttt taggtgaaac agtggagtct
    12121 ataataattt ttgcagttat tattataaac tctgttctgg gtgttgtaca aacaaaaaaa
    12181 gcagaaagtt cattagaaag tttgaaaaat ttatctgcac caaacgcaaa ggtaataaga
    12241 gacaataaaa aaatgacaat tccagcaaaa aatctagtag ttggagatat tgtattctta
    12301 gaagctggcg attacatacc agcagatgga aggcttatag aagcacaatc tctcaaggtt
    12361 gtagaaggga tgttaactgg agaatcagaa ccagttttaa agcatacaga aaaaataagt
    12421 gaagatgtaa gtttaggtga ccagaaaaat atggtattta gtggtgctgt agttgtttat
    12481 ggtagaggtt cttttgtagt gactggaaca ggtatggata ctgagatggg taaaatagct
    12541 gggcttttag aaagtgctga aaataaactt acaccattgc aacaaaaaat agaggatttt
    12601 agtaaaaaac ttggtgtagg tattgtaata cttgctcttt taatatttat aattcaagta
    12661 gcaagaaatt actttatggc tggaaatgga gaaatgacac aaattatttt agattcattc
    12721 atgttctctg tagcagtagc agtagcagct ataccagaag ctctatcatc aatagtcact
    12781 atagtattgg ctgttggaac aaatagtatg gcaaaaaaac aagccataat aagaaaatta
    12841 ccagcagtag aaactttagg ttcaacaagt gtaatatgta ctgataaaac aggtactctt
    12901 acacaaaata aaatgacagt agttgattat tatatgtatg gaactaataa tgatgagtta
    12961 gataaagaaa aagtaaacta ttcttatcat gctaaattat tgactatggt ttctacattg
    13021 tgtaatgact ctcatataac tagtgatggt aaagaagttg gtgacccaac agaagtggct
    13081 ttaataaatt attcaagtaa aaatgattta gattatgata aattaagaag taagtatatc
    13141 agaataaatg aaataccatt tgattcagac agaaagctta tgtctactgt aaatagtata
    13201 tatggagatt atataatgtt tacaaaaggt gctccagata ttgtgtttag tagatgtaaa
    13261 tatgctttga aaaatggttc aaaagtagat ataacagatg agataataga agaatataaa
    13321 cataaaaatg aagagttttc aaatagagca cttagagtat tagcttttgc aataaaagat
    13381 gtcccagaag aagattttat accttcaatt gatgacgaac atgacatgac attggttgga
    13441 ataatggcaa tgatagaccc accaagagaa gaagtaatgg atgcagtaaa agaggcaaag
    13501 agtgctggta taaaaactgt tatgataact ggtgaccata aaacaacagc agcagcaatt
    13561 gcaaaagaga ttggtataat ggaagaagga gacttggctc ttactggaaa agagcttgat
    13621 gctttgagtg aagaacaact atatgaaaaa ctagaaaata taagtgtata tgccagagtc
    13681 tcgccagaaa ataaaattcg tatagtaaaa gcatggcaac ataagaataa tataacagca
    13741 atgactggtg atggtgttaa tgatgcacct gcattaaaac aagcagatat aggaattggt
    13801 atgggaagtg gtactgatgt agcaaaagat gcttctgcta tggtattggt tgatgataac
    13861 tttgcaagta tagtaaatgc tgttgaagtt ggtagaacag tatacagtaa tataataaaa
    13921 tcaattactt atctatttgc aggaaactta ggagctatag tatccatagt atttgcagtg
    13981 tttgcaggtt ggtcaaatcc atttacagct ttacaacttt tgtttataaa cttagtaaat
    14041 gactctttac cagcaattgc attagggttt gaaaagtcag aaaaaaatat aatgaataaa
    14101 cctcctagac acccggatga aagtatacta gcaggaggaa cattgaaggc agttgctata
    14161 agaggttgta taataggtgt agttacaata attgcacaat atataggatt acaagtatct
    14221 ccagaacttg gagtagcaat ggcattttca acattgatat tagctagaat aatgcaaaca
    14281 ttacctgcac gttctaataa tcaaacagta ataaaattag gttttttcag caataaatac
    14341 gttataggag cagttttagt ctgtcttggg ctttactctt taacactaat accagtgcta
    14401 agaggaatat tctctatacc aacaacgttt ggattgtacc agttggggat ttgcctaggg
    14461 ttagcattaa tttcatcaat attaatggaa cttaacaaat taataaatag aagtgaagta
    14521 aaataatcaa aaaattaaag ggcatattga ttttaacaat atgcctttta taaatagtct
    14581 aatttattct atattatcga ttatttggaa tgcttttata acttcaggta attctttttc
    14641 ttcaggcatt ctaaatctta tgaaattttt atcaagtcca gtaaaatcac ttccagatat
    14701 aactcttatt tttaatttag caaactcttg ttgtaaatct atgtccttgt ttttatgttc
    14761 aactgtcata attgatacag tatcactagt ttcagctata ttaaggtttt tccatggaat
    14821 tagtaattga ttttttatat cttttgtttt ttcttttaat tcttctataa attgaacatc
    14881 atctaataaa tttgctgcaa tactacgaga tatttcagac acttcgtaag gagtagaaat
    14941 ctttttaata gggctaacaa gttgttctgg aagaactgca tatcctgctc ttagtcctgc
    15001 taaaccaaat ccttttgaaa atgtttttaa agcaattaca ttatcataat tatttagaag
    15061 cttgacagct gaattttctt ttggcatgta ttcaccataa gcttcatcta ccataactgc
    15121 tatatcatat ttagctgctt cctttactat attttcaatt gatgataaag gtataatttg
    15181 tcctgtagga ttgtttggat tgtctatgta aattacttta tattctggat ttattttttc
    15241 aataaattct ttttcattaa acttgaaatt atcctctttc tttaaaagaa cataatcata
    15301 agttgcacca tgcattttta tatctgtctc atattctgag aactgaggtg aatatcctaa
    15361 aactttatct cccttttcaa taaatagtct atttaaaagg taaataacat gtatagaacc
    15421 atctgccaaa caaattctgt cagtatctaa tgctataaaa tctttccaat aatcgactat
    15481 attatctttt aaagcaattg aatgaggata tcctcttatc atatcaaatt ttaaagcatt
    15541 aaaagcttct acagctttgc ttgagtaaga cacggtattt ataccttcac cacagtctat
    15601 ctcgaataca tcttctgaat gtccagattc tattgcataa ctttttttca actgcatttc
    15661 aatagctttg ttaattttta gtttcatact ttatcactcc ttatttagtc aataataaat
    15721 gttataaaag ttgctagatt taaaatatta tgaaaatata taatagtaaa aaatgaatat
    15781 aaatattata atagctcact ttatttaata tgattatata tcatatattg taaaatttct
    15841 agcattataa gactataaat tgaaaatatt cagaaaaata ctacaagaaa cgatttattt
    15901 tgctcttaaa ataagttaaa tgaatgttat taattttgta aattgaaata gtttaagtat
    15961 aaagttatga tataatctaa taagggtaat tctttagatt atattaagga aatataggta
    16021 tacaataaaa agttatggag ttggattatg ggatataaat taattgtaac agatatggat
    16081 ggaactttat taggcaataa tcataaagtt acagatgaaa ataaaactgc actacaaaaa
    16141 gttataaaaa gcggtattaa tgtaacttta gcaacaggaa gagccttcga ttcagctaaa
    16201 tgcaatgtag attttcttaa agaggacatg ccaataatag cttgtaatgg ttcattaata
    16261 agagagcaaa atggaaatat aatatattca aataagattg atacaagaac atgtttaaac
    16321 attttagatg tattagataa atacgatatt tactaccaat gtaatagtat agattctatg
    16381 ctttcgaaaa aaatagaagg acgtgaagat agattaagtg tatttttagg ctctgaaaca
    16441 gaagtaattg taaaagatga tttgagagaa gaaatcttta aaaaagatat actaaaattt
    16501 gtaatcatag aagaaaaaaa tccatctatt ttggatgaaa taagaaaaga attgagaaaa
    16561 gttcaaggaa taaagataac ttcttcatgg ccaaacaata ttgaagtcat gaatgaaggt
    16621 gttgataaag gtaatgcagt taaaatttta gctgaaaaaa tgaatataga cagagaagat
    16681 ataattgcat ttggagacaa ttacaatgac atagaaatga taaagtttgc tggtcttggg
    16741 gtagctatgg gaaatgcaga agaattgatt aaacaagaag ctgattatgt tacagataca
    16801 aatcaagaca gtggagttgc aaaggcaatt tataaattcc tagagttaaa agaatcactt
    16861 agctcttaaa acagtatata ataggaaaaa ataggttgac tattttgtag atagtaatta
    16921 taatacaaat agataaaaca taaaaattta gattcagtga aaaagagagt agatatagag
    16981 aaaatctcag cgaattgggg gatggtgtga gcctaatgat aacactttat attgaaaaga
    17041 actttggaga atactacttg aaattcagta tggtagtagg taatctcgcc ttaagagaat
    17101 agagtgggta aacattgttt accaactaga gtggtaacac ggaatatttc gtctcttggt
    17161 ctttttgacc aagagatttt ttagtttcat tgagttacaa gacttataat taaaatacaa
    17221 aaaattaaaa tatgaggtga aagttatgaa attatggggc ggacgcttta gaaaagcgga
    17281 aaatcaactt atggaagaat ttaataaatc ttttggctat gattgtgtac tgtacaaaaa
    17341 agatatcgaa ggaagtgtag cacatgttca tatgcaagta aaatgtggac ttttaacaga
    17401 agaagaagga aagtctataa cagaaggttt aaaagggata cttgaagatg tagaaaatgg
    17461 aaagttagta ttggatggag agtatgaaga tatacatagt tttactgaga taaatttaat
    17521 tcaaagaatt ggagatgttg gcaagaagct tcatacagca cgtagtagaa atgaccaagt
    17581 tgcagttgat atgagattgt atgctaaaga aaaagcaaat gatttggtgg gtttaatatc
    17641 tgaatttaaa gctactataa aagatgttgc tgacaaaaac ccagtgatga tgccaggcta
    17701 tactcatctt caaagagctc aagtagtgac atttaagcat cacttaatgg catattatag
    17761 tatgtttgat agagatgaga aaagaataaa aaatgctata gaaatattag atgaatcacc
    17821 tttaggctgt ggagctttag caggaactac gcatgatata gatagaagta taacttgtga
    17881 acaattagga tttaaaaaag tagtagataa ttttatggat ggagtaagtg atagagatta
    17941 tttactagag ctaatgagtg atttttcaat tataatgatg cacttaagta gattaagtga
    18001 agagttgata ctttggagtt ctcaagaatt tggatttgtt gagatagatg atttatatac
    18061 aactggaagt agtataatgc ctcagaagaa aaatccagat ggtgcagagc ttataagagg
    18121 taagactggc agagtatatg gaaacttatt tggtcttttt acagtaatga aaggaatacc
    18181 attagcatat aataaagaca tgcaagaaga taaagaaggt tttttcgata gtgtacatac
    18241 tcttgaaatg tgtatacaaa taatggatag aatgatagcc acactaaagg taaatgagga
    18301 taaaatgaaa caagcagtaa aaaatggttt tttaaatgca acagaagttg cggattattt
    18361 ggttaaaaat aatgttgctt ttagagatgc acatggtata gtaggaagta tcgttatata
    18421 ttgtgaagat aataaaaaag caatagaaga tttaacatta gaagaattac ataagttttc
    18481 agatgctttt aaggaagata tttatgattt tatcgactat gagagtatat taaataaggg
    18541 aataaaaaag aatttaaaat aatgtattta tttttaaaag ctgtaaattt gaatgttatc
    18601 atttaaattt acagcttttt attgtgtaag attttattat ataaaagtaa gaaacttaaa
    18661 aggagtagta tttaaaataa atacaaataa aaaattatta ttaaaatgat aattaatatc
    18721 aataagtatt gacaaaatta tttctaaata ctattatata taatataaat aataatgaaa
    18781 atcaatatca aataaatggg aggaaatcta atgaacttat tagtgattgg tggtcatgaa
    18841 agaatggaaa aagattatat ggttatggcc aagaaaaaag gttataagac aaaggtatat
    18901 actaccatgt catctaaatt aaataattct ataggaagac cagatgcagt tgtcatactt
    18961 acatcaactg tttcacataa gatgtctaga acggttgagt ctcaagctaa aaaacaagat
    19021 atattaatcg taaggcataa aaatagtagt aaggtagcat ttaatgaatg tctagatatg
    19081 gttgatgaat gtctgggaaa ttgttcaaaa tgtaagctta aagacaaaca aaaacaaaaa
    19141 aatatatagg tataaaaaat acataaacat aaaattaaaa gtactgagat aatagttata
    19201 attattatct cagtactttt agatttataa ggataagtat attataaatt ttttattata
    19261 atttccaact acttgttact ttttcatatt ttttttactt ctagcttttt ttcttttttt
    19321 cttctttata tttgaagcta ttataaataa aactaaaagc actagaatac ctgcaataat
    19381 cttaacaaaa gtcattaaaa tattatttcc agtaaaaaat ggaagactgc tatcaagatt
    19441 attttttgca attaagtcta tactgttttc tagtttacca ttattatata catttaaagt
    19501 accaacctta gctccttttt ttataggcaa atcaatttta tttaactctg ttttggtatc
    19561 ataattgcct tgagattcat ctttttctaa gaatatccta taactatttt ttggttcata
    19621 aatcaattca tgttgttttg taaacaatac ttttttagta tctgtgtatt tttctttatc
    19681 aacaatagta gcacacttaa agttatcaaa tccataatca atcagaatac gtgagtctaa
    19741 atataaatca gcattagttg aattaaatac agcaacgata actcttctgc catcttttac
    19801 agcactcgat aaaagacatt taccagcagc atcagtatat ccagttttta taccatcaac
    19861 tatatcatat tttatattta taggttgacc tttgtatgta atcttatcat gtgatgtcaa
    19921 aaacttatta gtgtttacaa agtatctctc ataaggatat gcttttgtag cttcaaattt
    19981 aagtgactta gtttttacaa tttgtctaaa agtatcatta ttcatagctt ctctagctat
    20041 gagagccata tcatgagctg ttgtgtagtg atttgggtct ggtaatccat gaggattatt
    20101 aaaatgagta ttagtagctc caattttttt tgcctcagag ttcatgaggt caataaattt
    20161 ttgtacattg ccaccaccaa catatctagc caatacaaaa gcagcatcat tagcagaatg
    20221 aaccaaaaga gcatctagta actgctttac agtaaaagat tccccatttt ttagatacat
    20281 actagagcca tctatttgag gtaaatcttt aatttctatt acttgattta aatctttaac
    20341 ctcctttaga actaaacaag ctgtccatac tttagttgtt gaagctggat aaagcttaga
    20401 atttccatcc ttattatata gtattttgcc agactcatag tccattaaaa ctgcatgttt
    20461 agcaactata gctggttcat tatctgcaaa agagatatta gagcttccta aaaatgaagt
    20521 aaatatcaat aaacagatta ataaaagtga taaatttctt ttcattgtga gcctccaaat
    20581 tttaaattca tcttttagta tagcatatat ttgtcaataa ttaaaagaaa ggagtgatta
    20641 atatatgaaa aaaaaggtaa aatttatttc gttttttgtt ttatttattt taataatatc
    20701 atcaataatc gtttataaat cgcttatttt caaagatgat atttatgttg taagtaaaaa
    20761 aaatttacct aatgaaaaaa cacatgttgt agagaatgaa attaaaggtg aagataataa
    20821 tcacaataaa gtaactaaag acatagataa aaattttcaa aataataaaa taactatata
    20881 cataagtgga gcagtaaata gacctggaat tgtaactata gagtcagaca agagacttta
    20941 tgatgctgta gaattactag gaggaacgac aaaagaagca gatttaaatg gagtcaatct
    21001 atcagtaaga ttagaagatg aacaacatta cataatacct aaaataggtg aagcaacatc
    21061 tgttacaagt aatgatgatt ccaataaacc aaatcagaag aatgagtcaa gaggtgagcc
    21121 taaaaataaa tccaataaaa atatatctaa cgaatcaaaa gtaaatataa atgtagcgac
    21181 tatagaagag ttagattctc tacctggggt tggagaagct acagccaata aaattttaca
    21241 acacagagaa gaaaatggac agtttagttc aattgaggaa ataaaaaatg taaatggtat
    21301 tggagataaa aagtatgaaa atataaaaga tttaatttgt gtagactaaa atataataca
    21361 aaacttacat atagatatgt tattataaat atgtatgtat atgtgtgtac atgtggaaat
    21421 ttagtaaagg aggcactttt atgaaaaaag aacaaaaaaa acttacagag atgacaacag
    21481 caggtggttg agccgctaaa atagggccag aggttctggc atcagtttta tctcaattgc
    21541 ctaaaaatga taatatagaa aatttattag taggactaga tacagcagat gatgctgcag
    21601 tttataaatt aaatgatgat atggcactta tccaaacttt agacttcttt acaccaatgg
    21661 tagataaccc atacgtattt ggtcaaattg ctgcatcaaa ttcactatca gatgtatatg
    21721 ctatgggagg caaacctttg gttgctatga atattgtttg ttttccatca tgccatgata
    21781 tggatgtact tgcggaaata ttaaaaggtg ggtttgataa agtgaaagaa agtggagcac
    21841 ttttggttgg agggcatact gtagatgaca aagagcctaa atatggtctt tcagtttctg
    21901 ggattgtaag tccaaataaa gttttatcaa atgctactgc aaaacctggt gataagttaa
    21961 ttataacaaa accaattgga gtgggtgtat taaatacagc catgaaagaa ggtatggtag
    22021 aacaacatat agcagataaa gtaatagaaa taatgataca tttaaataaa tatgcagcaa
    22081 tgagttttga gaaatttgat gtaaattcgg ttacagatat aactggtttt ggtctacttg
    22141 gtcatacttt agaaatggcc aaagcttctg aagtaagtat tgaaattgaa tcaaaacatg
    22201 taccaattat tgaaggtgca atagaaatgg cacaaatggg aattatacct gctggtatgt
    22261 ataaaaatat gcattatgta tctaaagatg tagaagttgt aggaaatata gaagttgcag
    22321 ttcaagatat tttatacgac ccacaaacat caggagggtt gttaatttca gtaaaagaag
    22381 aacttgcaga ggaacttgta aaagatatga aattaaatgg agcaatagaa gctaaaatta
    22441 taggttctgt agttccaaaa ggggaaaaat atattacagt tttataaaat attatagaga
    22501 aagagtgatt gttttgagca aaagagaatt gtttgcaatg ttgccttcag ttgatgaagt
    22561 attaagtgat aatagaattg tagaaataat aaatgaatat cctagaagtt tagttttaga
    22621 atctgtgaga gaagtaatag atttaaaaag acaatttata ttaagattaa aagaagatgc
    22681 atcaaactct gtaacaatag agtttggtga aataatagaa tctgcaatag agagagttaa
    22741 gttaaattac tcattatctt taaaaaaagt tataaatgca actggtacag ttattcatac
    22801 aaatttaggg aggtctttac ttagtgaaga tataaaagat gaattatggt gtgctgcttc
    22861 aaggtattca aacttagaat acgatttgga taatggagaa agaggttcaa gatactctca
    22921 tttaactagt acaataaaaa gacttactgg agcagaagat gtactagttg taaataataa
    22981 tgcagcggca gtacttcttg tacttagcac aatggctaaa ggaggagaag caattgtatc
    23041 tcgtggagaa ttagttgaag taggtggttc atttaggatt ccaagtataa tggctcttag
    23101 tggtgcagaa ttagttgaag ttggttcaac taataaaact catttgaaag attacaagga
    23161 ggccattaca gaagatacaa atgtattgat gaaggttcat acaagtaatt atagaataat
    23221 gggattcaca gagagtgttt ctatagaaga attggttaat ttaggtaaaa aatataaact
    23281 acctgtaata gaagacttag gaagtggagt ttttatagat ttatcaaaat acggtttatc
    23341 ttatgaacca actgtattgg attctattcg acaaggtgca gatgtagtaa cttttagtgg
    23401 agataagatg cttggaggtc cacaagcagg tattatagtt gggaaaaaag agtatataga
    23461 aaaaatgaag aaaaatcaac ttactagagc acttagagtt gataagctta caatatgtgc
    23521 tcttgaagca acattgagaa tgtatttaga tgagacaaaa gctatagaaa atatacctac
    23581 tttaaaaatg ttgacatata aaattgaaga attagaagta aaagcaaata aactatttga
    23641 aaaaataact gctttaaact taaatgcaaa cataaatata gaagatggat tttcacaagt
    23701 gggaggaggg tctatgcctc ttgaaactat aagtacaaag gtaatatcaa taactcctga
    23761 acatatgaat gtttcatctt tagaaaaaaa actaagactt agtgaagcac atataatagc
    23821 cagagtgtat gataataaat atgtactaga tgtaagaact atatttgatg atgaatttga
    23881 tgtaatagtt gcggaattga gaaaagcatt taattaaata aatttgagct aataaatatt
    23941 aaatttagaa gtataagtat gaagttttga ttaaaaaata gctacacaca cttgtgactt
    24001 tagtcatagc aggtttagag ggggcaaaaa atgaaaaatg tagtaattgg aacagcaggt
    24061 catattgacc acggaaaaac tactcttata aaagcattga ctggaagaga gactgataca
    24121 ttagatgaag aaaaaaagag aggtatttca ataaacttag gatttactta ttttgattta
    24181 ccaaacaata gaagagctgg aattgttgat gtcccaggac acgaaaaatt cataaaaaac
    24241 atgttagctg gagcatcagg aattgatata gttttacttg ttgtagcagc agatgaaggg
    24301 gttatgccac agacagttga gcatttagat attctaagct ttttaaatat caaaaatgga
    24361 atagtggttc taactaagtc tgatactgta gatgatgaat ttagagagct agtaaaagaa
    24421 gatatttgtg aaaaaataaa aggaactttt cttgaagatg ctgaaataat tgaagttgat
    24481 tctatatcta aaagaggtat agacactctt attgaaaaaa tagataaaat gagtgatgat
    24541 atagaagata aaaatgaaaa ctctccagca agacttaata tagacagagt attttcaata
    24601 aaagggtttg gtactgtaat aacaggcact ttaatagaag gaaagataag tatagaagat
    24661 gatttagtta tatatccaca ggtattaaaa acaaaaataa gaagtataca agttcatgga
    24721 gaaaataaag atacagctta tgcaggtcaa agaacagcaa tcaatatatc aaatgtaaaa
    24781 gtggatgata taaaaagagg agatgtttta gctgcaccaa actcattaga ggaatcaatg
    24841 atgttagatg taaaattatc attggtaaat catttagata aaggattaaa gcactgggat
    24901 agattaagat tgtatcatgg aactagagag atactttgta gagcagttcc actagataaa
    24961 gaattaattg aaagtggaga aagtggctat gttcagttga gacttgaaga aagcatagta
    25021 tctaagaaag gtgatacgtt tgtagtgaga cgttattctc caatggaaac tataggtgga
    25081 ggtgtaataa ttgacccatc tcctaaaaaa cataagaaat ttgatgaaaa agttatagaa
    25141 gcattaaaaa taaaagaaaa aggcgaacta aaagatataa tagaagagta cttaaaaagg
    25201 aatttaaaaa attatccaaa tataaaagaa ataatgagct atagtggtgc tcatgaagaa
    25261 gatgtaaaaa gagcgcttga agcacttata tcagaagata aagtatttat tataggaaat
    25321 atgtatatgc atataaatca atacaataaa ttaaaagaaa atacaataaa gcttttaagt
    25381 gagtaccata agaagtatag gcttagaaaa gggatactaa aagaagaagt aaggtctaag
    25441 atagagagta attttaagac tagggagatg gatattttat tagaaaaatt gtctacagaa
    25501 aatgctataa aaatagaaaa taatatagta tctctattag attttgaggt tattttaaat
    25561 gataagcaaa aagaaatagc aaaaaaaata gaaaaaagat taaaatcatg tggagtttct
    25621 tctatactga caatagatga agtttctgaa ggaaatcata actatgctga agtattggag
    25681 tcaatgatag gtaataaagt agagaaatta gatgatttgt atataatgga caaagatatt
    25741 tatgaaaatg caaagaacat tttgataaat tatataaaag aaaataaaga aataacatta
    25801 ggagaataca gagatttgat tgattctagc agaaagaatt gcatgattat attggaaaac
    25861 ttcgatagaa acaaaataac taaaagagta gaaaacaaaa gaattttgtt ttagcagaaa
    25921 atgtctgttt ttgctttaag gtataaagaa ggaattataa tgtcgagtat cgtataatta
    25981 attagtataa aatattaatt aagaggaagt gtaaagtatt gaagaatata atacctgatg
    26041 taaaaccaga tataaaagaa agagaagaca tgagcctttt atttataata atgggctcag
    26101 tacttttatt atttattata aaagatatag agaaatatga aactttatta catgccatat
    26161 atacgatatt tttagtagct acaggagtaa ttatatttaa tactacaaaa tttaaagtag
    26221 attcattttc tacattttta ggattaattt ttatagctac tggagtattg gagtgtatat
    26281 atttatttaa ttccttgggg attaaaacta agtctataat ggagattaac atcacaatat
    26341 cagcaataac agatttattt cctatactgg gtgtatactt aagttttaaa tttgttaagg
    26401 ataacaaaca aatttattca agtataattt tatttatagc aactgctaca ttaacaataa
    26461 catctttatt cataatatca ggaatttgtg actatctggg aaatggtaaa tcacaatatg
    26521 ctttaggagt tataatatct atattcataa taattatttc tatcattagt ggaattgaat
    26581 taaattcaag ttcaaataaa ggtaaatggg aatatgatga aaaaaagatt tttaacagaa
    26641 taatagtaat aataatttta tcaagagttc caaatctatt gcatatagtt attgataata
    26701 gaaatgtaga gcggatatta tcacagataa taataaatgt agccttatat tatttatata
    26761 attacatagt atctaagaat attaagaaaa cagcactgga attgcatgat acgaatgaag
    26821 aattaacaaa aaaaacggaa agtttaaaag aaaagaataa aaaattaatc tatgagacag
    26881 aaaagataga ggaacttaag ggaatactta tgaaaagaga atctaggcta caatctacat
    26941 tagatgtagc agtaaatagc atagtagtgt ttagcaaaga tggagaaatt acatatgcaa
    27001 ataagagctt tagaaatact tttggagact ataaagaaca agataaattg actgataaaa
    27061 taaaaaactt caatgatttg atagaaagca ttcattatgt gtttgttcat gagaagaatg
    27121 cagagaaact tattagtaca agcgataata aagtttatca agctatattt gcacctttaa
    27181 ttattgcttc acaaacagaa ggagtattgt gtgtactaga agataagact aagaaaaagg
    27241 agtacgaacg caaacttatt gaagcaaata agagaagtaa agatttttta gaaagtattg
    27301 gagatggcat agtagtgctt gaaggtaata acaaaatata tgttaataat gcatgtaggg
    27361 aaatttttaa aaataattta gagagtattg atttttcatt agtatgtaaa agtgaagaaa
    27421 gtatagaaaa aagatatgta atagatggtg aagtaaagta tgtagagatg agtttttctc
    27481 agtatactaa tgctggtact aataaaacta ttattgtaat acgtgataca acctctcgaa
    27541 aaatatctca aataaaactt gaggaaagtc aatcatcata ttcaagattt atagatatat
    27601 tacctgatgg catatgctta ttaaaagaag atttaagtat aaactatgca aataagtcgt
    27661 ttttagatat gctagctttt acaaacatag atgatataaa agattctaat attaaactat
    27721 taatgaatgc gaatactgaa gaaaaaatga aatttacgga taatatggct aaagttttaa
    27781 atgaaaatag aagtatactt ttactggaat atgaattgat aaatagttat gatgatatag
    27841 tagaagtaga attaagtgca ttaccttttg ctatatacaa tactagatac ataatgctta
    27901 ttataaaaga tttagtacat aagaaatatt ccgagcaagc agaaaaagaa ctgcttgaaa
    27961 gatttaaaac agataaaatc aaaacggagt tttttgcaaa tatgtctcat gaattaaaaa
    28021 ctccactaaa tgttatatct agttcaaatc aactggtaga ttctttctat agaaatgaaa
    28081 agataaaaga ttacaataac aatattaaat ctcatgtgga tttagttaga caaagttctt
    28141 ataggcttca aagattaata aataacatta tagatttaac aaagatggag tctgggttct
    28201 atacactaaa acttgcgaaa tacaatattg tatctgtaat agaagactta tttatgaata
    28261 tagaagagta tgcacttaga aaagacataa aaattctttt tgatacagac cttgaagaaa
    28321 ttaatgttta tatagacaag gttgaaattg agagaattat gttgaatcta ttgtcaaact
    28381 gtattaagtt tactgataat ggaggctgga tatatgtaag tattcattat aaaatagata
    28441 aagtaatcat aagtgtaaaa gacactggag ttggaatacc acaagataaa ttagaattga
    28501 tttttgaaga attttcacaa gttgataaga ctttatccag aaatactgaa ggaagtggaa
    28561 tagggcttgc tatagttaag aatctagtaa gtcttcatgg tggagatata gaagtagtaa
    28621 gtgaagtgaa taaaggcaca gagttcttaa tttcacttcc aatgaaggga tttagtagtg
    28681 aacattacac agaagataag agaatatata atattcaaga aaaaataaaa atagaatttt
    28741 ctgatatata ctattaaaaa tgattatgag caaaaataaa ataaaaatac tatttgttta
    28801 aatatttaag ttgtaagaaa taggctatca taaggtaaat tattatgata gcctattttt
    28861 agctaggttt tatatctata tgtgagaaca tataatctct aaattacctt caaaactaaa
    28921 atctccaaag tcgtttggca gtataaaatg catacccttt tttaaattat attctacaga
    28981 attgtgtata agttttccat ttccatccaa cacactgtat aaagaaaaag gtgtattgtg
    29041 tgtaaaatta cattttccaa aaacatctaa tttataaaca ctaaaaaatt cgtttgacac
    29101 aaatgttgtg catttaaagt ctgatgttga tacgatttta tagtcagtat cgaaatttat
    29161 atgaggaaca ttcgtaacat ctattgattt ttgaacatga agttcacgct tattacctga
    29221 attgtcagtt ctatcataat catagactct ataagtagta tctgagtttt gttgagtttc
    29281 taaaatcaat gttcctttgc atatagcatg tatagtacca cttggaacat agaaaaagtc
    29341 tccctttttt atattaacct ttctaagtag tttgtcccat tcattattat ttaccatatt
    29401 tacaaattct ttatgagatt ttgcattatg acctattata atttctgcat catcagaaca
    29461 atctattaca taccaacact ctgtcttacc taactctcca ttttcatttt tcttagcata
    29521 ttcatcattt ggatggactt gaactgataa attatcattt gcatccaata tttttgtaag
    29581 taaaggaaac ttatcaccag gtgtgtttcc aaataattct ctatttttat tccacaattc
    29641 agatagtttt ttacctttat attttccatt ttctattaag caatctccat ttttatgaga
    29701 gcttatagcc caacattcac cagttgttgg cgaatctatt tcataattaa atttatcttt
    29761 aagtgcagta ccaccccata ttctatccat aaaaataggt tttaaaaata atggttccat
    29821 aatcctgttc ctcctatgat attaaattta tcctacaaat aattatatat aaatagatta
    29881 taaaatcaat agtaaaaaag ttttgtccct tgaaatgaca taaagtataa ggagttatta
    29941 aataagataa actatgatat ataatataaa gttctaaatt ggagtgataa ttatggagtt
    30001 gtttaaggca gaaagcaaaa aatatgaaaa agaattttta aaattattgg agcaatgggt
    30061 tagtatacct tcattttatg atagaaaaac tgtatctaaa gatatgcctt ttggaaaagg
    30121 agtatatgat gcattaaact ggtttgaaaa tctaggtaga gaaaacaatt tcaaagtaaa
    30181 aaatatagat aatcatgctg ttcaaattga atatggaaat ggtaaagaat atgtagatat
    30241 atttggacat tgtgatgttg taaatccagg tgaaggatgg gatagtgaac catttaaact
    30301 aaatattata ggtgataaat tagttgctag aggtgtatct gataataaag gacctatgat
    30361 agtgaatttt ttagcactaa aaatgataaa ggatttagat attaatttaa aaagaaaggt
    30421 tagattaata gctggaggaa atgaagagag tggatttaaa tgtataaagc attattatag
    30481 caaagagcca tatggtgtat gtggatttac tccagatgct aaatttcctg tattaaatgg
    30541 agaaaaaggt ggagcaataa taaagttaat ttcaaatata gacgataaat ctttatatat
    30601 aagtggtgga atagagttta atacaatacc agataaagtt tatataaaga atgtagaaaa
    30661 attgggtaaa gataatatat gttttgatat taacaatata tcgataaatt atgataatgg
    30721 caattatatt gtgtatggaa aaggtggtca ttcatctaaa cccgaaaaat caataaatcc
    30781 tatattagct acaattaaac ttttgtcaga aaatattgat gaaaaatgga caaaagattt
    30841 atataagttg ataaatcaag ataatataaa tggaaactta tttggtttaa atattgaggg
    30901 gaaatgtggg attttatcaa tagtgccaac aataataaat atagtagatg gaaaattaga
    30961 agtagtttta agcgttagat atccagaaat attaaccatt gaagatataa taaaaaaatt
    31021 taatttgtat atggagcaaa ataatattaa taaatttgag cttattgggg aaaatttaaa
    31081 gcaagcaaat tatatagata gaaattcaaa acttgtaaga agtttgcatg atatatatat
    31141 aaagtattct ggagatttga aaaatgatgt tagagtaact agtgcaggaa gttatgcttc
    31201 tgaaatgaat aattcagtaa tatttggttg tgaatttcct gatggtagct ttggcaatgt
    31261 ccatagtgcg aatgaatttg ctagcttaga tagatttata actgcaatag gtatatatgc
    31321 agaggcaata attacattat gtaataaaat ataaatacaa aatgtccctt atatagacat
    31381 attatatata caattaaaat tttacttgat atataattga aatcaaagag aaggtgatac
    31441 aatgataact aagaaacatg caataatagt aaagagttta agcaataaag atggatatat
    31501 gacatcaaac gaattagcta tcaaattgga tgtgtctact aaaactatta aaagatatat
    31561 agcagattta aatagtgttt taagcaaata tgatttggta atagcttctt ctagaggtat
    31621 aggatacaag ttaagcggtt ctaaaaacaa tatagccaga gctgttaaag aagcaaataa
    31681 atatattgat ggttttttag atgactctga agagtcaaga atgtcaaata taatatgtat
    31741 gcttattaat agaaattata tgagtataga agcaatggca gaagaattaa atctaagtat
    31801 agccgctata aataaattgt ctagtaagct taaaagaaaa cttgaaaagt atgacttagt
    31861 tataaaatct aaaccttact atggaagcta tatacatggt gaagaaatta atataaggca
    31921 attaattact gactatgcaa taaaattaga tgaaaaaagt aaagtgaagg tgtttttaga
    31981 tgacattagt gaaaacgata tatactgtat agaaagtatt ttagaaaagc acttaagaga
    32041 aaaagataca ataatttcag ataaagattt taatcttttg ctttcaaaaa taattgtttc
    32101 tgtatttagg agtaagagag gtcatagtaa caatataaac ttaatggata caagttatag
    32161 atttcataac tactacttta tagaaaatct aatgaaagaa atatctgata aaattggatt
    32221 taaacttatt gaagatgaag taatttatat ttcaaattat tctggtgtaa tagcttataa
    32281 aggtacacaa ggaagaaaaa acacaagtga aattgaggaa cgaataagta gtgttataag
    32341 tagtgcactt caagatatat tccttatttc aggtagtgat tatacaaaag atgatgagtt
    32401 tatggttgca atctctgacc atataaagag atttttaaat agagctagag ctaatgtaaa
    32461 atcaaataat cctttacttc atcaaataaa agagaagttt cctatagctt ttaatttagc
    32521 cgtgttttta tcaaataaac tagaaactga gtttaattta aaacttgatg aagatgagct
    32581 tggatatata gctatccact ttgctgcttc aaatgaaaga atgaaaaaaa atactagtaa
    32641 gaaaatttgt atagtttgtc attatgggat aggaacagga caacttattt ctgaaaagct
    32701 aaaacagaat ataagtgatt taagtgttgt aggtgtttat ccagttagat atcttgatat
    32761 ggctataagt caagatgttg atttgatagt atctaccgtt gaattaaaag gatatgaaaa
    32821 acctgtctta tatatagaaa atatatttga tgatagctta attgaaaacg taaacaaagc
    32881 cttttatgaa aaggaagaaa gaagaaagat aataagtaac atgtttgatg aaaaagcttt
    32941 tttcagtata aaggctagta ctaaagaaga ggtcataatt tcactttcaa ataaactaaa
    33001 agaaagagat tttatagagg aaagttctat aaaaagtata atagatagag aaaatatttc
    33061 atcaactgaa ataggaaatc tagtagcaat acctcacaca atagtgaagg gagataaaaa
    33121 atctataata ggagttggaa tacttgaaaa tccaataatt tgggataagc aagaggttca
    33181 gctagtattt atggtatttt ttaatactaa agaaaagcat aatttctcga tatttaaata
    33241 cctttataat tttataaaag atgaaggagg agtaagagga attattaaaa tatgtgactt
    33301 taataaatta atggcactga taggtaatta aggaggaaat taataatggg aacaaacata
    33361 tttaacaaag aatatgtatt tttaaatgtt gatgctaagt caaaggttga agttttaaaa
    33421 tttatatcaa aaaaagctaa agacttaaat ttggcagaag atgagagctt agtatatgaa
    33481 ggtttaatgg ctagagaaga acaatttact acaaatttag gagaatctat agcaatacct
    33541 cacactaaaa atgatgcaat tgaaaatcca gcagtagtag tattgaagtt taatgaagat
    33601 gttgtttgga atgaaggtga ggataaggtt aaattagcaa taagcttact tatgccagga
    33661 aaaagtaaag agaatattca tcttaaatta ttatcatctc tttcaagaaa attaataaat
    33721 aaagaattta aagattctct tttaaaaagt gataatgttg aagaaatatc taacttaata
    33781 aatgaagctt taggtttata attaaatact aaggttataa aataaaaaaa taattaacta
    33841 aaaataggag gaatttgaaa tgaataaaaa attagtagca ttatgtgctt gcccaatggg
    33901 actagctcac actttcatgg cagcagaagc aatagagcaa gcagcaaagg cattaggtta
    33961 tgaagcaaaa gttgaaactc aaggtgctga tggtgtacaa aatgagttaa caagagatga
    34021 tatattagga gcaactatga taatacatgc agtagcaata acaccagaag gtatggaaag
    34081 atttgatggt tgtgaagttt atgaagttga attacaagaa gctataaaaa atgcagaagg
    34141 tgttataaaa gaaatagaag aagatttagg aatataataa atttattgtg gaggaaatag
    34201 aatggcaatt aagaaaaaag ttataaacag ttcaggtgct tctacaacag gaggaaataa
    34261 tgtaggaaaa tctactccat ctagtccaac tcaatcaaag ggaaatggaa aatggaaaga
    34321 aataagtaaa catataatga caggtatatc atatatgata ccagttttag taatgggtgg
    34381 acttataggg gccttatctc aattaatacc atatgctata ttaggacttg acccatctgt
    34441 aggtatagtt gatgctatga actcaggaga atttacaggg ttcaagctta gtcttttaaa
    34501 tatagcacaa ttaatgtcaa atttcggatt tactttgttt ggatttgcga taccactgtt
    34561 tgcagcattt tgtgcaaact ctataggtgg aaaaacagct ttaattgcag gatttatagg
    34621 tggatatata gctaataaac ctgtaggtgt accacaattc attgatggac aatggactga
    34681 agttgttcca gttgcatcag gatttttagg tgcaatacta atagcattca taataggata
    34741 ttttgtaaaa tggttaaata aatctataaa agtttctcat aactggttag cttttaaaac
    34801 aacattttta ataccattaa tagcgtcatt agcttgtatg gtattgatga tatttataat
    34861 aactccattt ggtggattaa ttaatgaaag tatgaaaaac ttcttaacag cagcaggagc
    34921 agctggagaa tatgtatatg caacagcttt ggcagcagct acagcattcg acttaggagg
    34981 accaataaat aaagcagcag gattcgttgc acttggttta actacagaga atgtacttcc
    35041 aataacagca agaacaatag ctatagttgt tcctccaatt ggattaggat taacaacatt
    35101 attagataaa agattagtag gaagaagagt atatgataga caattctatc aagcaggaaa
    35161 aacttctata ttcttatcat ttatgggtat atctgaagga gcaataccat ttgcacttga
    35221 gagaccaggt tttgttatac ctttaaacat agttggaagt gtaataggag ctattacagg
    35281 tataattcta ggagcaattc aatggttccc agagtcagct atatgggcat ggcctctagt
    35341 agataactta tttggatata taataggtat agcagttggt gctatattca tagcagtagg
    35401 aaatatattc tatagaaata aattaataaa agatggtaaa cttgttgtag attatatcga
    35461 ctagtattta atttttacta tctataaata actttggctg gtaatatggt tttaatcaat
    35521 attaccagcc ttacctatat gattaattaa aggcggtgga gataagatat ggataagata
    35581 gaaaaagtaa gagagtattt tagagaatat gatatagatg gatttcttat aaatagtcct
    35641 acaaacaagt tttatgttgg aaatttattt agctcttcag gttatgtttt tataacgaaa
    35701 gaatcacaat atataatagt agattttaga tattttgaag aaattaaaag aaaatctagt
    35761 ttgtttaatg tagtattaat ggacaaaact agaactcatt ttgatataat taatgatatt
    35821 tgtagagagc agaatataaa ggaaattgga tttgagggaa atgaagtatc ttttgattta
    35881 tatagaagta tgagtaataa gttaagtgca accttaaaat cagttgattt atctacatta
    35941 agggaaacaa aaaatgaaga cgaaataaaa tatataaaaa aagcttgtga aatagttgat
    36001 gctacatttt atcatatagt agactttata aagattggta tgactgaaaa gcaagttgaa
    36061 aatgaaattg ttagaattat aaaagattta ggtggtcaaa aagaatcttt cgatacaata
    36121 gttgcatctg ggcttagagg agcattacct catggaaaag caagtgaaaa aattatagag
    36181 tatggagatt ttgttacttt tgactttggt gctaagtata ataattattg ttcagatatt
    36241 accagaacca tttgtatggg tactataaat aaagagttag aagaaatata taatatagta
    36301 agaaaagcta atgaagagtg tataagagtt ttaagaccgg gaatgactac tggagagata
    36361 gataaagttg caagagacat aattggttca tatggatatg ccaataattt tggtcataat
    36421 ctgggtcatg gtgtaggaat tatggttcat gaatatcctg ccttagctcc tgaatcaaat
    36481 gaagtattaa aagaaggaat gattgtaact atagagcctg gtatatatgt tccaagtctt
    36541 ggaggcgtaa gaatagaaga tgatgtactc ataacacaag atggatgtat gagattgact
    36601 acatctacta aggatttaat agttgtaagc taaaaataaa ttagataaat attttataac
    36661 aataagaaaa gaagaggtaa atttaaatgt ataattttga tgataaacca gatagagtaa
    36721 gtgaaaagtg tagaaaatgg gatttaaata ttataagaga taaatttgga gatattagag
    36781 aagattttgt accaatgtgg attgctgata tggattttaa aataccaaca gaaatagaaa
    36841 acaaatttat tgaagctgta agaagaggtg tatttggata tacatactgt tatgatgaat
    36901 tttatgattc agttattggt tggcaaaaaa atatgcatga ggtaaatgta gaaaaagaat
    36961 ggattacatt aacttatgga actgtatcaa cacttcatta tgtagtacaa gctttttgta
    37021 agaaaggtga tagtataata ttaaacacac cagtttatga cccctttgaa tcatcagcaa
    37081 aaaaacaagg tgtaaatgta atttgtaata cattagatgt tgtaaatgat agatattata
    37141 taaattttga taaattagaa gttcaaatta aagaaaataa gcctaaatta atgatgtttt
    37201 gtacaccaca taatccatct ggaaggatat ggacaattga agaaatgaca agggtagcaa
    37261 ctatatgtaa agaaaataat gttattttgg ttgcagatga agtacatgca gagcatattc
    37321 attatggtaa atttaattca atacttaaga ttggaaaaga gcttttagaa aatgttattc
    37381 tactgacatc accaaataag ggctttaatc ttggtggatt aaagacttct tattcaatag
    37441 ttataaataa gaatataagg gataaattta gaaataaatt aaagcaaaat tctataactt
    37501 ctccaaatgt gtttggaata ataggattga taaccgctta taatgaatgt catgagtggt
    37561 taagtggtgt aaatgaatat ataaaatcaa attatgaact tttagaaact tgggtaaata
    37621 aatataataa gataaaattg atgaaaatgg aatcatctta cttagcttgg atggatataa
    37681 gtggattagg tataagtgca agtgagttta cagataaatt ggctatagat acaggagtat
    37741 tattagaaga tgggagtcat tttgtaaaag atggtgagaa gtatgttaga ataaatttag
    37801 gaactcaaaa ggaaaatgta attgaagctt taaatagaat ggatttattt ttaaagtctt
    37861 tataaaaatt tggttgaatt agtattttat ataaatttaa gctattaata tggtatatac
    37921 ataagtagaa tttgttttag attacttaag tatataccat attttatata aaaattaaat
    37981 ttgcattagt catgtgataa ctatttaaat tcatatgtat tttgtatgta taatagatat
    38041 aattattatt taaacttatt gtttatttaa tttttcaata gaaaaagatt tatctgtaag
    38101 tttaaaatta tcttttatac ccttagttaa atatactttc ttatccttag ttatgaatat
    38161 agcatctaca ttatcaattt cattaattaa atccatacct ttttcaagac caagtccaaa
    38221 tgtagaagtg gataaggcat cacaattaat agatttatca gaaattattg taacactact
    38281 tagattgttt tcaaaaggat accctgtaaa tggatttaac atatgatgat atatttttcc
    38341 gtctttttcg ataaatcttt cataaattcc agaagtaaca acagatttat ttgtggtttc
    38401 tatgtttcct atggattcac catttttact agtggggtct tgaatgccta ttttaaaagg
    38461 tttatcattt ttaccatctt ctaaagtaaa tatatttcct cctaagttta ctaaaccttt
    38521 ttttacgtcc tctgatttta aataggctac aattttatca gcagcatatc ctttagctat
    38581 ggcacctaaa tcaattttca tattttcttt ggttaatttt atagatttat ttttttcatc
    38641 caatatgatg ttcttataat caataagagg cagaagtttt tgtatctcag aatcacttgg
    38701 aactttagct ttatctgttc caatagccca taaatttgat aatggaccaa ttgttatgtc
    38761 aaaatatcca tctgataatt tggaatattg tatagcttct tttacgacga aaaatgtatc
    38821 atcagaaact ctgacaaatt ttttaccagc attatcattt attttagaaa catcacttcc
    38881 tggtatatga gtgctcattt tgttgtcaat atatcttaaa attgaatcac attcatttaa
    38941 gattttatca cttttggatt cttttacatt aaatatagta acttcgttta ctgtaccaag
    39001 gtagtagcta gttttactat atttagtatt ctggcttgaa ctcctaaaaa atgaaaataa
    39061 aattaatgct aaaatcaata tcacaaaaat aaatgtgatt tttttcttat tcatggtata
    39121 ttcctcctat gaaattgtgt tattatttta ttataacata atttcatagg aggacaaaaa
    39181 atgaagaaaa aagacattct tcttatttta ggcattttgg ttgttattat agcatgttat
    39241 gggataataa atgttataaa ttctaaaaat gctggaaata ttgagattta tgtagataat
    39301 aaattgtata aaactgtttc aataaatgcc aaagaagaat ttaaaataga gaatagaggg
    39361 gggtataaca tagtaaagat tcatgataaa ggggttgaga tagtagatgc ttcatgccct
    39421 gataaggttt gcgtacatac tggatttata aacaagccaa gtcaaagcat agtttgtata
    39481 cctaatagag tgagcataaa aataaataca aatgaaaaga atgataatca agaagatata
    39541 atttctaaat aaaataataa aaataattga aaggaattgg tgataattat ggttaactta
    39601 ggaaacgatt gggatgaatt attgaaagaa gaatttgaaa aagattacta cttaaactta
    39661 agaaaatttc ttatagaaga atataaaaca cgccaaattt tcccaaatat gcacaatata
    39721 tatgaagctt taaaacacac ttcatacaaa gacacaaaag tcttaatatt aggacaagac
    39781 ccttatcatg gagataatca agctcatggt ttagcttttt cagttcaacc acaagtgaaa
    39841 actccaccat ctctattaaa tatgtataaa gaattaaaag atgatttagg ttgttttata
    39901 cctaataatg gatatttaat gccttgggca gaccaaggag tgttactttt aaatactgct
    39961 cttactgtta gagcgcatga agctaattct cataagaaca aaggatggga aattttcact
    40021 gatagagtaa tatctatttt aagtgagcgt gaagacccag ttatttttgt actatgggga
    40081 agtaatgcac gtaaaaaagt agagttgatt gatacaagta aacattatat tttggaagca
    40141 cctcatccaa gcccattgtc agcaagtaaa ggcttttttg gatgtaaaca cttctctaag
    40201 ataaatgaaa ttttgaaaaa gctaggaaaa gaacctatta attggcagat agaaaatata
    40261 taaaagtaaa aggtaacaaa atgataacaa aacaacaata aacaatgaca ttgttgtaac
    40321 taatagaaat atgcttaaat agttgaaaat actatgtatg catatttcta tttttttgtc
    40381 aagttgacaa ctcttgaaaa aatagttaca atttagttac acaaaaaaca caaataaaaa
    40441 aagaaactta caaagcagaa ttttatgaag caaaatatat agtgtaaaaa attttaggga
    40501 ggaaatttta tatgaatttt aaaaaattga gtaatgctaa agtctcggta atggcagttg
    40561 ttttatcagt tggaatatta tcgggatact ctgcgcttaa taaacaagta acattagtag
    40621 taaggggaga agaaagaact atatcaacat ttagttctaa tgtaggagac cttttagaat
    40681 ctcaaaacat tagctatgat gcaaatgata tagtaacttt agatgaaaat acaaagttat
    40741 ctaatggaga taaaatagaa gtaattgatg taaaggaaaa aacagttgta gaaactaaag
    40801 acattccatt tgaagttaca gttgttgaag atggctcttt attaaagggt gattctaagg
    40861 ttaaagaaga aggaaagtct ggtaaaagtg aattaatata taaagaaact tatcacaatg
    40921 gaaagaaagt tgaaaaggaa tttgtgaaaa aggttgttaa agctgagcca gtaaataagg
    40981 ttgtaagcaa agggacaaag gtagaaatgc aagtagcctc atcaagggga gaaagttcaa
    41041 gaagaatggc ttcatcatca tctagtagca gtagtagtaa caatggtaaa aatatgagag
    41101 tggtagcaac tgcatatact ggtcatagta taactgcaac aggaacaaaa cctaagtggg
    41161 gaacaatagc tgtagaccct aaggttatac catatggaac taaagtttat atacctcaat
    41221 ttgacatggt gtttactgca gaagactgtg gtggagcaat aaaaggaaat aaaattgata
    41281 tctacatgaa tgattcaaaa acggtttata actggggaag aaaaactata gatatacgta
    41341 tattaaaata gttaaataat ttatattttt actggaaatt atattttgat tatatagaga
    41401 agaaactctt tatagattct taaaatataa tttccagttt ttattttgga tatgtaataa
    41461 aacaggtaaa ttttaaccat gtatagataa aagctattta aattataatt tactatcaga
    41521 taaatctata aaaattgttg caaagagtca aaatatatga tattatagat ttcgttgaga
    41581 aggatttcat tatgggagtg gatgggtgcc ggtgtgccct ctagtcttca aaactagtat
    41641 gaggggttaa gagcctctta ggtgggttcg attcccacgc attcccgcca atatgcaaaa
    41701 ggtatctttt aattaaaaga taccttttta aatttatttt aagataattt atttttaata
    41761 atatataatg ttttgtatta tataatgtaa attttctaat actattatat atttagataa
    41821 aaatctagga gtgtggaaag atggaaaata attttacaac gagaactagt tttctagttg
    41881 gagatgatgg gatagaaaaa cttaataatt caaatataat agtatttgga gttggaggag
    41941 ttggtagttt tactgttgaa gctttagcaa gagctggagt aggcaatatt actattgtag
    42001 attttgatga tgtagatata accaatataa atagacaaat accagcccta cattcaactg
    42061 taggaaggta caaagttgat gtcatggaag aaagaatatt agatataaat cctaatatca
    42121 atataaaaaa aataagaagt ctttataata aagatactag tgacgaaata ctaactgaaa
    42181 gatatgatta tgttgtggat gcgatagaca tggtaagttc caaaatacat ctaattgaaa
    42241 cttgtgaaaa aaaagggttg aaaataataa gttctatggg tatgggaaat aaattagacc
    42301 caactaaaat agtcgttaca gatatacaca aaacaagtac ttgtcctcta gctaaagtta
    42361 tgaggaaaga acttagggat agaggtatca aaaagttaaa agtagtatat tctactgaac
    42421 aaccaataga acttaagaag aaagtaatga atggtagaaa agtaacacct ggtagtgtat
    42481 catttgtccc atctgtagga ggtttaataa tcgcatctgt aatagtaaat gaactattag
    42541 gacaataagt tttaaatatt ttaatttata aattttatat acaaatttga aggctagaag
    42601 catgaatata aagcaatttt atattcatac ttttagccta attattttat attaccttat
    42661 tttatgtagt tgtagtttta ttttttagat tatgtatcta tttcataatt atattttgta
    42721 aatataattt ctccatcact ataaaaaaca atatctccta ttctatctgt tctataaatt
    42781 gatacatggt tttgttttag attattaata acctccctat gtggatgacc atattgattt
    42841 ttatatccac atgatattgc agctatatct ggactaattt tttttataaa ttctaaagat
    42901 gaagaagaat tacttccatg atgacctatt tttataaaat taatattatt taatttaaaa
    42961 gaatgtaaaa tttctttttc attttcttct tcagcatcac ccataaataa aaaagaatta
    43021 tcgttaaaag ttaatttgaa aactatagaa tttaaattag attcttcttg tatatatgat
    43081 ggacttaata catatatatt tatattgtta tctatgttta aaacatcatt tttatagaga
    43141 tgctgaatag ataagttttt atccgtacaa gaatttatta gattttgata agcctcacta
    43201 tcagttgatt gttcaggcat atatatggag tttacattaa atttttttat aacattatca
    43261 agacttccta tgtggtctga atctggatga gttgctatta taatatcaat agtttttatt
    43321 cttttttgtc taagataact aataattata ttttcgctat cttcatcacc tccatcaatt
    43381 aatatattct tatttgtggg tgtttgaact aaaatactat caccttgtcc aacatcaatt
    43441 atatgtatag atagtagaga ttttttgtca cagccgatta aaagtgatat tataataagt
    43501 attgataaaa aaattttatt tctcaataaa aaatcactcc attcattaca tgtttatata
    43561 aatttatgat gggtaatata agatatatat gacttattat attttatatt ttaaacaata
    43621 taaatgaaat taataccata ataaatttgg agttttttat atatacaaaa taaaattttt
    43681 aaaattaagc aataaaaaag gtaggaggat tttatgtttt tcaaagatat tgaactaaat
    43741 tcaaaaaaag agttagaccc atattttgat ttagtagatt atgaagcttg tgagtactgt
    43801 tttagtactt tatatatgtg gcaacatgtt tataagacag gatattatat aggtgaagat
    43861 tttgccgttt tagttgggga atacgaagga gatagtttct caatattacc acttgctaaa
    43921 aaagataaac ttccagaagt tgtagatttt gtccttgaat atttttctaa aaataataaa
    43981 aaaatatact taagaggtat tactactgaa gtagtagagt ttttaaaaga aaaatatcca
    44041 ggtagatttg agtatataga agaaagagat ttatttgact atatttatga tgcagaaagt
    44101 ttaagaactc tagctggtaa aaagaatcaa aagaagagaa accatataaa ctatttctta
    44161 aaagagtatg caggtagata tgaagctaaa ttattagata aagaaaactt tgacgagtgt
    44221 ttagtactaa tgaaggaatg ggaaagtaat aaggaagaaa ataatgaatt tgatgaaagt
    44281 atggatgacg aattaatagg tatcaaaaag atatttaacc attatgacat attaaaagat
    44341 aaggtaaaag tgtttggtgt ttatgtggat ggaaaattag aggcttttag tataggagaa
    44401 cttttaaatc caaatatggc attgattcat atagaaaaag ctaatcccga tataagaggt
    44461 ctgtatccat ttataaatca acaatttttg gtaagtgaat ttaaggacgt tgagtttgta
    44521 aatagagaag aagatttagg tattgaagga ttaagaaaag ctaaactttc ttatcatcct
    44581 tgcagatttg ttgaaaagta cagtgttagg gaggcttaat agactatatg gagattaggt
    44641 atgctaaaga agaagagata gaaagtataa aagagatttg gagttattgt tttaacgata
    44701 cggagagttt tatgaagtac tattttaatg ataaatataa atctgaaaat actgtagtag
    44761 ctttagatga aggaaaaatt atttcttctc ttcagctaaa tcagtataag ttattactaa
    44821 atagcaaggt atataataca tcatatgtag taggagtttc tacacttcca gagggaagag
    44881 gagctggata tatgaacaaa gtaatgaaat ttactttaaa cgaattgtat aaaaaagggc
    44941 aattagtttc tatattgatg cctattgact atagacttta tagaagattt ggatatgagc
    45001 attgttacga ccagattgaa tatactatta acacagatga tttaaaaaat tttaagtcaa
    45061 gtggaaaaat gataaagtct aatttatcac aaattgatga tttgatacga atagatagaa
    45121 cttttttaaa tgaggttaat ggtaatgtgt taaaagatga acattattat gaaaacttat
    45181 ttaaagagat tcaaagtgaa gatggatttt tatacattca tgaaggaaat gaaaaagatg
    45241 gatatatagt ttattttctt caggaagata aaatgtttgt cagagagtta ttttataaaa
    45301 atatagatgc ccttaaatca atgcttaaat ttatatataa tcacaatact caatgtaaga
    45361 tagttactat atctactcct acaatagata agataagatt tatattagac aaccctaaag
    45421 atagtgatat aaaaataaaa ccttttatga tgggaagagt tataaatgta aagaagttta
    45481 ttgaagatat agatattgag aaggatatta atagctcatt taatttatta atagaagaca
    45541 agtttataga tgaaaataat ggtctattta aaatttctat acagaataag aaggtatctg
    45601 tagagcagtt agataaaaaa ggtgcagaaa agcctcaaga agattttgat attaaattag
    45661 atataaatac cttaacacag ttatcatttt cttatataga tgtaaatgaa gctattttct
    45721 taaatgatat aaaagatgtt tcagaagaaa cactagaaac acttaattgt attttttcta
    45781 aaaaaaatac ctatataaat gaatatatat aggtagaata cgttaaaaga ttttaaaaat
    45841 cttttgacgt atttttttgt ttagataaca atttatcttg tttaaaaatt ttctatttga
    45901 aaataatcta aataatatac ttttatatgg gagtgattat aattcaaata agaagaccat
    45961 tattaattat attaatgttt gtggtagcta tatcatttat cattacaaat aacacaaaaa
    46021 acgacgaaga acttaatgat aagataataa ctatacaagg ggttgtcaaa ggaaaaattg
    46081 aaaagaaaag atacaatcag tacaaggtag gggtttttct aataaatgat tatacaaaaa
    46141 ataaaaattt gaagattggt cagaaagtta atattacagg taaattcaaa agtttagata
    46201 aaatgaagta tgatgatttt gattatggaa ggtatataaa aagtactgga tataaaggta
    46261 tagtgtatat aaataactat aaaattattg ataaaaataa actttactct ttaatagggg
    46321 aaataaagtt ttatattagt aaaacttata ggtacttata taagaaaaac tcagatttta
    46381 taaattctat actattagca gaagttgaaa atttgactga ggaacaaaaa gaaattttta
    46441 ctagaactgg aactagtcat gtaatatcta tatcaggtct tcatacagga atattatgtg
    46501 taattatatc ctttctatta agaggtataa ataaattgta taaattatta atactgtcga
    46561 tttttataac tttatattgt ataatggtag gtgcgtctcc atccataatt aggtctatag
    46621 catttgttat gattttttat ttatcaatat ttattgatag aaagaaagat ggaatatcag
    46681 cattgtctct aattgggata attttaatta tgaataaccc ctatgttata tataatgtaa
    46741 gttttcaatt gtctttttta gctacacttt ctatattaat attctataat aaaataaata
    46801 gtattataaa attaagtatg gtatcattga caatatcatc aaatatatta actttaccta
    46861 taatttatta tacatttaaa ggtataccat tgctttcaat tataggaaat ttaattatag
    46921 taccatttgt gggagtaata atgtatttaa gtattgcaag tttaatcgta tttaaagtaa
    46981 gtgtagtaat agctaaaatt atatcttttt ttaatagtac attgatagaa agcatatttt
    47041 ttttactaga aaaaatcagc aatttaagtt ttgcatacat taatatagaa agtcctaaat
    47101 tttatattgt tgttatttat tacataggag tgttttttta catattttac atagaaggaa
    47161 aagagataaa ggagcaagag aatgaattac aaggatatta taaggaatgt aaaagagaaa
    47221 aactttgaaa aaatgtatct tttttatgga agagagtatt atttaattga gaatgcaata
    47281 aaagcattta aagatagtct aaacgaagga atgcttgatt ttaatttaga tataatagat
    47341 gggaaggaaa tagtcttaaa tcagcttata agctctatag aaacacttcc atttatggat
    47401 gatagaaaga tagtaataat aaaagacttc gaactattaa aaggaaagaa aaagaatttt
    47461 acagatagtg atgagaaata cttaatagag catttagata atattccaga cactacaact
    47521 atagtttttg ttgtatatgg agatgtagac aaaagaaaat cattagttaa gaaaatagga
    47581 aacaatggaa ttgtttttga ttgtgataag ctttctgata tggatttatt taaatggata
    47641 aaaaaatctt ttgctttaaa tgatgttata atagataatt ctcaaatcat gtattttatt
    47701 gaacaagaag gatacagaga taagagcagt gaaaagacat tatctgattt agaaaatgaa
    47761 ataaataaaa taagttcctt tgttggtaaa gggaataatg taaccaatga cgtaattgat
    47821 aaattatctc aaaaaaaagt tgagaatgat atatttaaat taatcgatta tattggagaa
    47881 caaaatgctt ctaatgcaat gaaaatatta aatgatatga tacaagaagg cgaatctgta
    47941 ttaggtatat tttctatgat agcaagacaa tttaaaataa ttatgcaagt aagacagtta
    48001 caacttgatg gatattcaac aaaacttata gctgataaat taaaaatgca tcaatttgtt
    48061 gtaggaaaag cattaaagca aactaaaaat ttttctgatg atataattgt ggaaatatta
    48121 aactatatat tagaaagtga ttataaaata aaaacaggtc ttataagaga tactttagca
    48181 gttgaaatgt tggttagtag atactgtaaa agagaagcaa tttaaaaaat ctatatatta
    48241 ataaaaaata aatagcaaga tatgttatag ttcagagtag atgtaagcta aattaagttt
    48301 gatatacaat caactataat atgtctaact atttattttt tattgtataa atttacaaaa
    48361 taaaaaaccc ctacataggg gttattatat ataagaaata taaactggta attaagcgtt
    48421 cataccgttt aatttttgag ctaatttagc tacttttcta gctgcagcat tcttatgtat
    48481 agttccttta gaagctactt ggtatattct tttttgagcg tattggaact ttgcaacagc
    48541 atcctctctg tttccagctg caacagcatc ttcgaatctt cttattgcag tttttatttg
    48601 agattttcta actctattta aagcagtttt cttttcgata acacttattc ttttcttagc
    48661 tgattttata tttgccaccg tgttcacctc cctgtatttt tttatcatat tcatcgtcaa
    48721 catattagat tttaacagaa ataaatctaa aaatcaagga ttattattta tatattttaa
    48781 taatgttttt tattttgatt ttttagactg taaattgtgt ataaataatt tgcaatatct
    48841 tgtttttaaa ttggtatatt ttgtgtttta aatattttga catatataaa ttaatatttt
    48901 atttttgtaa caaatatttt gagataatac aaactattaa catacatttt gatagtattt
    48961 ccaataatta taagaaaata caagaaatta ctcatttttg taaatttaat gaaaaattat
    49021 aaaaatataa atttatatgc ttaattaaga taatttttcc aataatcaag ataatttttt
    49081 tcattagtat gtatgataga tgcatcaaac aatataacta atatgttaga aggttatatg
    49141 taattaatct cagtgtttat atagaatagc atttgtagca tttattatag ttgtaattac
    49201 tacttatatc atagacttga atagtctaaa aaaatagact ataatatttt tatagactaa
    49261 agaaagggct gataagatgg acatagcaaa actaatttta aatcctgcaa ggcttcgtat
    49321 cttacaatat attcgacttc atggcagtgc tcgcacatct gatattgtaa agtatttaaa
    49381 tgatattcct cgtgcaacag tttatcacca tgtaaaaatt ttagaagaga acaatatgat
    49441 tgaagtaatt aaagaaaatc gtgtgcgtgg tactattgaa aaggtttatg cactaaagga
    49501 atatacaaca tctatggaag gagagacctt cgttgcactt tctacagcat tttatgtagg
    49561 tctgatgcag gaaatgaatg aatactttag cagaaaagag caagaccata aaaaggataa
    49621 tgtatttttc tcttcagcat tactatatat gagtgataat gaatatgaaa atcttcttaa
    49681 aagtatagct gatttattga aaccatatat agaacaaaag cctaaatcaa atctaaaact
    49741 tagaaaactt tcaattatat cttctccacc tgtggttgat aatgataaaa caaaataaca
    49801 tgaatcttat ttttataaaa ggaggttgta tatgaaattt catgaatttg gagataaaaa
    49861 caaaccacat attatgttga ttcatggggg agggaatgcg tggtggaact atttgcgaca
    49921 agcagaagtt ttgtctaaca actatcatgt gatacttcct acattggatg gtcatggaga
    49981 agaatatgaa acaacataca tttctacaga ggatacagca gataagttga tatcatatat
    50041 agatgaaaat tgcagtggac atcttttcgc tttgtgtggg gtatcacttg gtggtcaaat
    50101 tgtcatggaa cttttatctc gaaaacctaa tattactaaa aaagcaatta ttgatggtag
    50161 tatttgctac ccaagacctc ttatggcaag gttttgtatt gctggtattc gattttttgg
    50221 aggacttttg tttagtgaga aggcttgtcg ctttcaaatt gctatgatgc caaaattgtt
    50281 acctgaaaat atgcaatatc cagatgaaat aaaagcgtat tatatgacag atatgccaca
    50341 tgtaagaaaa gaaacattct ataatatgta tcgtacttat atgatgaact atacacttaa
    50401 agagaatgtt aaaaaaacca cagcacaagt aatgtactgg tatggagaaa aagaaatgaa
    50461 gtgcgttaaa aaatctgcaa aaatgtttca gtcttatgta ccctcttgtg agatttatga
    50521 agctaagggc tataatcatg ggtatttatc gctttatctt ccaaatgaat ggttggaaat
    50581 tgcagaacca tttttccaaa aggaataaag gtgttaggtg attattgtat aattattgct
    50641 gtttttttat atagtaaata aattaggtat aaatataaaa aagttatttt agttaatata
    50701 ttagtgaaag cttaatagga aaattataaa tagattaaag gtagcgccta tataaatagt
    50761 tgctaccttt ttttgcttgt aaataaatga tagataagaa agtttactga caaatcactg
    50821 actaaaccca attttcttac attgaaagcc ttaattttac ttgttttaaa taaatttaca
    50881 atttcttgtt ataaaatact aaattttgtt aacaattata attagcattt aatttatact
    50941 ggaggtttat atgattaatg taagaacaga tttagcttta gaagctagtg aaatgtgtga
    51001 aaagtctcaa gaaggaagta gtattcctgg tgtaaaaata gaaactaaag aattagaaaa
    51061 ttgcattgta actaaagttg aagtaataga caggcaaggc gcagagataa tgaataaaga
    51121 tataggaaag tatataactc tggaaagtaa tcttatgaag tttgatgatg atgagtcgag
    51181 agaagaaatg ataagttatt taaaagagga gttagtagat atatttggcc aagacaaaaa
    51241 taaaaaaacc ctggtaattg gtcttggaaa tagaaatatt acttctgatg cgttaggacc
    51301 aaagagtgta tcaaaaacac ttgtaactag acatcttttt aaaaattata ataaagacta
    51361 tgatgatgat ttcactgagg tatcagcttt aagcccagga gttatgggtg ttactggaat
    51421 agaaactagt gaaatagtaa agtcattggt tgaaaaagta aaaccagata gagtagtagc
    51481 aatagacgca ttggcttcaa gaaaaatgga aagagtaaac tctactatac agatatcaac
    51541 agcaggaata tcacctggtg gaggtgttgg caatactaga aaatctctaa ctaaagaaac
    51601 tttaggcgtg gatgttattg caataggcgt tccaacggtg gttgatgcag caactcttac
    51661 aatagatgtc ttagatatgg caatagacaa tttaatagct caatcagaag aaacagaaag
    51721 tttttatgaa atgttgaaaa aacttaaaga agaagaaaaa tatcatctaa taaaagattc
    51781 gttagaccca tatgacaaga acttaatagt aacaccaaag gatatagatg atacaatcga
    51841 aaatctatct ataataataa gtgaaggctt aaataggtct ttacatccag gtagattggt
    51901 ataaaataag gaggagcaat tatgtttaaa aaatgcatta aagtagtaac cttgactttt
    51961 atattagcat gcattttacc aggaaaatca ttagctttga atcaggatga ttttttaaag
    52021 tttttagtaa attcatctta tccagaagct aaagtcgaag gaaatgatac agaaaataaa
    52081 aagaataata aaaataaaga aacaagcaaa gaaaataagg aagaaagtaa agaagaaaac
    52141 actaaatcta aagatgcaag taaagttgat aataaaaaag agagtgaaaa agaatatata
    52201 aaactttatg ttggtaaaga aaatgttcca gatattgagt caaaaaattc agacacaact
    52261 gaaactaaca caacatctag ttcagattat aaggatgatt taagagtaac caaagaaaat
    52321 ccaagaattt tgatatatca tactcatgga tgtgagactt attcaaattc acctgatgga
    52381 aattatcatt caagagataa gaaaaattca gtaatggaag taggaagtgc attaactagt
    52441 gctttagata gcaaaggatg gggtgtagtc catactacta aataccatga ctatccatct
    52501 tataataact cttatgcaag tagcttaaaa acaatacaaa gtatattacc taaatataac
    52561 tcagtggata tagcgataga cttacataga gatgctaggg atttaactaa tccagccact
    52621 aaggaaaaag accatttaaa atatacaact atgataaatg gagagagagt atcaaagttc
    52681 ttctttgttg taggaggtaa aaatacaaat agaaaacaac ttagagcttt agcagaagat
    52741 ataactgctt ttgctgagaa aaagtatcct ggtctagtat ctccaatagt agaaaaagac
    52801 tatgctagat tcaatcagtt tgcagttaag aatcatatgt tagtagaaat agggaataac
    52861 gcaacaagtg tagaagaatc taaggctact acaaagtatt tagccgagat tttagatgaa
    52921 tattttaaac aaaaaaatta gagctttaag ttataattag gagcatgcta aatgagccga
    52981 ttagaaaaaa atatagaaag taaaagtaaa ctaagaaaat acaagtatat tatgagaatc
    53041 ttatttttga ttactatgat tataggtaca tcagtttgtg tatttatagt agacagtaat
    53101 gcaaaaagta tgctaggaat gaaacaatat gattttccaa gtattaaatt tgatataaca
    53161 aaatttgatt ttgacatacc aaattttgat atacatgaaa tcataaaaaa atttaataag
    53221 tagtaataag tgaataaggt ttattgatta aaataagcct tattttagtt atatattttt
    53281 aatgtataaa ataaaaattc attaactgta ttttatagga tttactttaa gtttacgata
    53341 ttatataata tttagtgtta gacttgataa tccacatata atgtggataa ttttgtttag
    53401 tgccaattag tttatattca aaaattttaa atttggtata taaagagttt ttagataatg
    53461 taaaataata actataagaa taatcaagag gagggaattt taaggtggac aataaacaaa
    53521 gtagaactag aaattttagt ataattgcac atatagacca tggaaagtct accttagctg
    53581 atagattaat acaacaaact gggcttgtta gtgaaagaga catgaaaagt caattacttg
    53641 acaatatgga ccttgaaaga gaaagaggaa tcactataaa gcttcaaaac ataagattaa
    53701 tgtataaagc gaaggatgga aatgaatatt atttaaatct tatagataca cctggacatg
    53761 tagactttaa ctatgaggta tcaagaagtt tagctgcatg tgaaggagca ctattagtag
    53821 ttgatgcagc tcaaggtgtt gaggcacaaa ctttagccaa tgtttatctt gctatagacc
    53881 aagatttaga aatattacca ataataaata aaatagattt accaagtgca agaccagagg
    53941 aagtaaaaaa tgaaatagaa gatttaatag ggcttgattc aagtgaagct cctcttattt
    54001 cagctaaaac tggtttaaac atagaagatg ttttagaaga tattgtaaaa aatgttcctc
    54061 cacctaaggg agataatgaa gctccattaa aggcattaat attcgattct tactatgatg
    54121 cttacaaagg tgttgtagct tatgttagag tatttgaagg aactgttaaa aagggtatga
    54181 caataaaaat gatgaatacc aataaaaagt ttgaggtaac tgaagttggt gttatggctc
    54241 caggtcaaac tgaacttagt gaattatcag caggtgatgt tggttatatt gctgctagta
    54301 taaaagatat aagaagttgt cgtgttggtg atacaataac agattcaaat aatcctacag
    54361 aagaaccttt accaggatac aaaaaagcta ctccaatggt atattgtggt atatatccag
    54421 gagaaggaga aaagtatgag aatgttagag atgctcttga aaagcttcaa gtaaatgatg
    54481 ctgcacttga atatgaagct gagacttcag cagcactagg atttggtttt agatgtggtt
    54541 tcttaggtct tttacatatg gagataatgc aagaaagact tgagagagaa tttaaccttg
    54601 atataattac tacagctcca tctgttatat atagagttac aaaaatggat ggagaggttg
    54661 taatgataca aaatcctgca aacttaccag aaccatctga gataaagatg atagaagagc
    54721 ctatagtaaa aggagatata atagttccta aagactatgt gggtgttgtt atggagcttt
    54781 gccaggaaag acgtggaaat atgttaaata tggaatatat agatgagaga agagtaatgc
    54841 ttcactatga ccttcctctc aatgaagttg tttacgattt ctttgatgct ttaaaatcga
    54901 gaactagagg ttatggttct cttgattatg aggttaaagg ttatgtagca tctacacttg
    54961 taaaacttga tatattaatc aataaagaac aagtggatgc acttagtttt attgtccatg
    55021 agacacgtgc tttccctaga ggaaaggcaa tgtgtgagaa attaaaagga gaaattccaa
    55081 gacatcagtt tgctatacct atacaagctg ctgttggtaa taaggttatt gcaagagaaa
    55141 caataagcgc tctaagaaaa gatgttcttg ctaaatgtta tggtggggat atatctcgta
    55201 agaagaaact tcttgaaaag caaaaagaag gtaagaagcg tatgagacaa attggttctg
    55261 ttgaagttcc tcaaaaagca tttatgtctg tacttaaact agatgaataa attcttataa
    55321 atttagataa atatatttaa aagctagagt gattttagaa ttatttctaa attaatctag
    55381 cttttttgtg gtatatttta agtaaactca tagagaatat aagtaagaat atattaaaga
    55441 agaataaaaa ggtgatattt atgattaata tagaaattcc taaaaaagta gactatataa
    55501 ttaaagaatt agaaacgaat ggttatgaag cttatgtagt tggaggatgc gttagagatt
    55561 gtttattaga aagagttcca aacgattggg atataacaac tagtgctaga ccagaagtgg
    55621 tagtagaatt atttgaaaaa acaattccaa ctggtataca acatgggact gtaacagtta
    55681 tgattgaaca tgaaccattt gaagtgacaa cttatagaat tgatggaaat tatagtgatg
    55741 gtagacatcc agattcaata gaatttacaa ataacatagt taaggacttg tcaagaagag
    55801 attttactat caattcaatt gcatacaatt caaagacagg attagtcgac ccatttaatg
    55861 gctatgaaga tatacaaaat aagtatatac gttgtgttgg aaatccagta gatagatttg
    55921 aagaagatgc acttagaatg cttagggcag ttagattttc agcacaactt aattttaaaa
    55981 tagctgaagg tactaagcaa agtattcata aaaaagcaga tttgataaaa aatgtttcta
    56041 tcgaaagaat ccagacagag tttaataaaa tattagtatc tgattcatct aaattaaact
    56101 tattaaaatc aacaggtttg ttgaaattta taattcctga aatatgtgaa ttagaagatg
    56161 ttactcaaca caatccatat cacatctatg atgtacaaaa acatacttta atatctacag
    56221 aagtaattga agatgaactg tatttaaaac ttacaatgtt gtttcatgat ttaggtaaaa
    56281 aagtaactaa aacaactgat aaaaatggtg ttgaccattt ttatactcat agtagagaat
    56341 ctgtaaagat tgcaaagaag attttgagaa gacttaagta tgataattat actattaata
    56401 aagttttaat attaatacaa tatcatgatt atagaataga acctaaaagg aagataatta
    56461 aaaagttatt aaataaactt gaagatgttg aattatttga agatttaata aaagttaatt
    56521 gggctgatac acttgccaaa aacccaaagt atgcaaaaca aaagatatta aatcttattg
    56581 agtgtgaaaa agaatttaaa catataataa atcaaaagga gtgctttaat cttaaagatt
    56641 tagctattaa tggaaaagat ttgatatcta taggtataaa accaggtaaa gatataggac
    56701 atattttaaa taagatgctt gaaatagtca taaataatcc agaattaaat gaaaaagaaa
    56761 tactaaaaga aaaagcatta aatatatata cattttagat tcaaatataa aaaatttaat
    56821 tactttaaaa aattatagaa gaaaatactt atttagataa aataaataat ttttagagaa
    56881 tattttagaa gttacgattg tatgattttt aaacgcatag catttactct aggactatag
    56941 actaatataa aaaaaaactt tgggaatact aaaaccataa atttataaaa ttaatatttt
    57001 atatactgta aaacagtgag gagggacagt atgcatactc aactacaaaa gactattggt
    57061 ctttcagcgg ctctttctac tgttgtaggt atggttatag gttcgggagt tttttttaag
    57121 ccacaagcaa tatacactac aactaatgga gctccaggtc taggtattat agcgtggctt
    57181 ttaggtggtt ttataacaat aactgctggg cttacagcta cagaaatttc agctgctata
    57241 ccaaaaactg gtggtatgat gatttatatc gaagaaatat atggagaaaa gttaggcttt
    57301 ttaactggat ggatgcaaac tgtattattt ttcccaggta catcagcagc attaggagta
    57361 atatttgctc aacaagcatc tgaactttta ggaatgagcc caaataatat ggcaaatgta
    57421 cttcctatag caattggagt tatactattt ttagcgttat taaatataat aggttcatcc
    57481 ttaggaggaa aagttcagac agtagccact attggaaaga tgataccttt aattttaata
    57541 atagtctttg gatttattaa aggtcaaagt agtgaagtat taaatccatt tgtaggagat
    57601 ggtgtaaatg catcaaatgc acttggacaa gtattaatag ctactttgtt tgcatatgat
    57661 ggttggataa atgttggagc tatatcagga gaaatgaagt ctccagagaa agatttacca
    57721 agagcaatag ttggtggttt atcattagtt atggcagttt atattataat aaatatagct
    57781 tatctttggg tagtaccagc aagtgaatta gcaacagtta cttccccagc tacattagta
    57841 gcaacaagat tgtttggaaa tataggtgga aaagtaataa ctgtgggtat attaatatca
    57901 gtatttggga cattgaatgg atatttatta acaggttcaa ggatacctta tacattagct
    57961 gaaatgggaa ctttaccagc atctaaaact cttttaaaag taaattctgg aggttctcct
    58021 gtaaattcaa tactacttat aactgtgtta gcctgtgtat atgcattatc tggtcaattt
    58081 aatcttttga cagacttaac tatattttca atatgggtat tttatgtatt gacttttata
    58141 ggtgttatga ggcttagaag acaaaagcca gatttacaca gaccatataa agtcccttta
    58201 tatccaataa taccaattat agctatacta ggtggactgt ttgtaataat aaatcaaata
    58261 ttgacttcaa caattatatc tttaggtgga atatttataa cactattagg tttaccagtt
    58321 tattattata tgaaaaaaag ataactactt aatttaagtt agataaattt gggtaaaggt
    58381 gaaaattaac ttttatccaa attttttata ctgttataca atataagcat tatatattac
    58441 ttgatgtgtt attttgtttg atttataatt tagtaattga tgctttaaga taatttaaga
    58501 aatgaggaat aaaaaatgct aggactttat gtacatattc cattttgtgt gaaaaaatgt
    58561 aagtattgtg attttaattc ttataaaatg gacatagatt caaaaaaaag atatatagaa
    58621 gacttaaaaa tagaaatgaa attatatagt aataaattat ataaagataa taaatataaa
    58681 aataaagaat gctgtagtct aaataaaaat gacaaaataa cgagtatatt tgtaggggga
    58741 ggtacaccta gtatactaac atctgatgaa ataagagaag tatttataag tataaaagaa
    58801 atgtttgata tagatgagaa tgcagaaata actatagagt gtaatcctgg gacattaaca
    58861 ttagaaaaat taaaaactat gaaagagatt ggcataaata gacttagtat agggctacag
    58921 gccattcaag aaaagcattt gaattttata ggaagaatac atacttatga agaatttgag
    58981 aaaaactata aagatgcatt aagtgtaggt tttaaaaata ttaatgtaga tttaatgtac
    59041 agtcttccaa atcaaactct atgtgactgg aaggaaactc ttgaaaaagt tgtacactta
    59101 aatccaacac atatatcagc gtattccctt atactagaag aaggaactga actatataat
    59161 atgtatgaaa gtaataaatt tgagcttata gatgaaaatg tagatataga aatgtatgag
    59221 tatacaataa actacttaaa gtcaaaagga tataatcaat atgaaatatc aaattactca
    59281 aaagaaggct ataattgtga gcataatatt ttatattggg aatgtgaaca ttatataggg
    59341 attggagcag gagcttctgg ctatattaat gagaacagat acaataatgt agaatcttta
    59401 gaagattatc atttaagttt ggtaaaaaga gaaaaaccaa tacaagaaaa tgaaattctt
    59461 tctgagaaag atatgataga agaaaagata tttatggggc ttagaatgaa taaaggcata
    59521 aaatttgaag attttaaaaa aaaatttgga atagatttta gagagaagta taataagcaa
    59581 atagaaatgc tattagctag aaagcttata aatcagagct ttgaaggtat acaattaact
    59641 caaaaaggaa gagaaatatc taatagtgta tttatagaat ttatggaata atttttaagg
    59701 tgttgacaaa ataaatttat aatggtatat aatatatata atctttttag cactcgaact
    59761 tagtgagtgc taacagctag gaggtataat aatatggaat taaatgaaag aaaattaaat
    59821 atcctcaaag ctatagttaa agattatata gaaacagctg aagctatagg ttctagaaca
    59881 atatctaaaa gacatgattt aggtgttagt gctgctacca taagaaatga aatggctgac
    59941 cttgaagaat tagggtattt aattcagcct catacttctg cgggtagagt tccatctgaa
    60001 aaaggctata agttatatgt aaactctctt atgagtaaaa gcgaattgga tgataatgat
    60061 aagattctta ttgaacaatg tatgaatcat aatataaatc atataaaaga attgattcat
    60121 gaaacctcta agctattatc gcaactaaca aactatacaa ctgtagcagt tactaagagt
    60181 ttaataaatc aaagtgttat aaagcacata cagttggttg ctatgaatga caataatatt
    60241 ttacttatag ttgtaacaga taaaggggac ttaaaaaaag ctaaccttac aactaatgta
    60301 tatttagatc aatctaagtt aaatttgatt tctgataatc ttacaaggaa gcttttaggt
    60361 aaaagtataa cagatttgga tgataatcta attgctttta ttaaatacga aataagtgag
    60421 tattctggac ttatagatga gcttttaaat gctttgaact caaatatgaa agaagaagat
    60481 ttttctctat cattaaatgg agctactaac atatttagtt atccagaatt taatgatgta
    60541 ttaaaagcaa agtcattttt aaatatgttg gaaaaaaagg aaacaatagc tgacataatt
    60601 aaatcaaagg gaatacaaaa agacaatcta aatataataa ttggtagtga taatgattgt
    60661 gaactggcac aggattgtag tatagttaca gcaacttata atgtagatag agacttggtt
    60721 gggcgaataa gctttatagg ccctaccaga atggattatg ctagaattta ctctataata
    60781 aattatatga gtttattaat taatagaaag taaagaaatt ttaaattgcg aggtgtaaag
    60841 tcttggataa aaaaaatgag caacaggaag taagagaaga aaatgatact tctattaacc
    60901 aagaatcgga aactcaagta gagttagaag aggaagttgt taatgaagaa tgtgaaacat
    60961 caagtgaaaa aactgatgaa aaagaagttg atgatgaaaa tgtaacagat ataaattcta
    61021 agttagcaga aaaaaaattg caggatgagt tggatgaatt aaatgataaa tatcaaagac
    61081 ttcaagccga atatgcaaac tacagaagaa gaactcaaca agagaaagag acaataggcg
    61141 tatttgcaaa tgaaaaaata ataactgagt tgataccagt tatagatagt atggaaagag
    61201 ccttagatgc atgtgaagat aaggaagata ctatgtataa gggtataagc cttgtacata
    61261 aacaattaat agacactttg gtaaaatttg gagtagaaga aatagaagca gaatctaagg
    61321 aatttgaccc taatctacat ctagcagtta tgcaagaaag tgtagatgga gttgaagcaa
    61381 atcaaatagt aatggtactt caaaaaggat ataagctagg aactaaagtt gtaagacctt
    61441 caatggttaa agtttcttgc taagtataaa ttaaaggtta aattatttaa cctaatataa
    61501 aatattaaaa taagtaaaac ttaattatat aattactcga ggaggatttt agtcatggga
    61561 aaaataatag gaatagattt aggaacaaca aattcatgtg ttgcagtgtt agaaggtgga
    61621 gaagcacaaa taatagcaaa tagtgaaggt atgagaacta ctccatcagt agtagcattt
    61681 acaaaagatg gtgagagaat agttggagaa cctgcaaaaa gacaagcagt tacaaatgca
    61741 gataaaacaa taacttctat aaaaactcat atgggaactg attataaagt aaatatagat
    61801 ggaaaatcat atactccaca agaaatatca gcaataatat tacaaaaatt aaaatcagat
    61861 gcagaaagtt acttaggaca aacagtaaca gaagcagtta taacagtacc agcttacttt
    61921 acagatgctc aaagacaagc gactaaagat gctggtagaa tagcagggct tgatgttaag
    61981 agaataataa atgagccaac agcagcagct cttgcttatg gtatggataa attagaccaa
    62041 gaaaagaaaa tattagtatt tgacttaggt ggaggaactt ttgacgtatc tatacttgaa
    62101 ataggagatg gaacttttga agttttagct acagctggta acaatagatt aggtggagat
    62161 gactttgacc aaatagtaat agattattta gcagatgaat ttaaaaaagc tgaaggtgta
    62221 gatttaagaa atgataaaat ggctcttcaa agattaaaag aagcagcaga aaaggctaag
    62281 aaagaattat catcaacaat gagttcaaac ataaacttac ctttcataac tgctacagca
    62341 gaaggaccaa aacacttaaa tatagattta tcaagagcta aatttgaaga attaactaga
    62401 ggtttagttg aaaaaactat ggaaccaact aagacagctt tacaagatgc aggactttct
    62461 acaggtgata tagatgatgt attattagta ggtggttcta caagaatacc agcagtacaa
    62521 gaagctgtta agaagtttat aggaaaagaa cctcataaag gtataaaccc agatgagtgt
    62581 gttgcagcag gtgcttctat acaagctgga gttttagcag gggatgttaa agatttatta
    62641 ttactagatg ttactccatt atctcttggt atagaaacta tgggtaatgt aatgactaag
    62701 ataatagaaa gaaatactac aataccaact aagaagtctc aaatattctc aactgctgct
    62761 gataaccaaa cagctgttga tatacatgtt cttcaaggtg aaagaagtat ggcttatgac
    62821 aatacaactt taggaagatt ccaattaaca gatataccac cagcacaaag aggaatacct
    62881 caaatagaag ttacttttga tatagatgca aatggtatag ttaatgtttc agctaaagat
    62941 ttaggtacag gaaaagagca aaaaataact ataacatcaa acactaattt atcagaagca
    63001 gaaatagagc aaaagataaa agaagcagaa atgaatgcag aagctgacaa acaaaagaaa
    63061 gaaaaaatag aagcattcaa tcaagctgaa tctactatat atcaaactga aaaaacttta
    63121 aatgaacttg gagataaaat aagttcaggt gaaaaagagg atatagaaaa agctatagca
    63181 gacttaaaag ctgtaaaaga taatcaagat gctacagcag aagaacttaa gaaagctaca
    63241 gatgaagtaa tgactaagtt ccaaaaagta tctcaagaaa tgtaccaaaa agcagctcaa
    63301 gaacaacaag cggcacaagg tgcagaacaa gcacaagaca atggacctaa agatgacaat
    63361 gtagtagatg ctgactttaa agaagtagat gaagataaat aagctatata atatgtaaca
    63421 ctaaatgata attggaacat atatgatttg tgaaaatcat atagatgtta aagatatatg
    63481 gacaatacac atcataaaga atataatata tcatagatta tataacttaa agtagaatat
    63541 aagaattaat gatagcttgt agtttattat tctacaagct atcgttttgt aaaatgaagg
    63601 tggtgtcaat ttttgagtac aaaaagggat tattatgaag tcttaggtat cagtaaagat
    63661 gcagaagctc aagagataaa aaaagcttat agaaaattag caatgaagta ccatccagat
    63721 agaaatcctg gagataaaga ggcggaagaa aaatttaaag agataaatga agcttatgaa
    63781 gttctatctg atgatacaaa gagaaaaact tatgaccagt ttggacatga tggattaaat
    63841 ggtcaaggtg gttttggtgg tcaaggagga tttggcggtc aaggctttgg tggttttgaa
    63901 gatatgttcg gagatatatt tggtgacatg tttggaggaa gcttcggagg tggaagagca
    63961 agaagaagag ggcctcaacg tggtgcagat ataagacaga gtgttactat atcttttgaa
    64021 gaagctgcat ttggtaaaaa gatgtctata aaagtaaata gaagtgaaga gtgtgaagaa
    64081 tgtaatggaa ctggagcaaa accaggaaca tcaaagaaaa cttgttcaac ttgtaatgga
    64141 actggccaag ttagaactgt tcaaagaact ccatttggta atatagcaag ttcaagacca
    64201 tgtagtgcct gtaatggaac tggagaagtg attgaatctc catgtagtaa atgtcatgga
    64261 actggaaata caagaaaagt taagactata gaagtagata tacctgctgg tatagatgat
    64321 ggtcaaatga taaaactttc tggtcaaggt gaagtaggag aaaaaggtgc tccaagagga
    64381 gacttatata ttgtagttaa tgttaagtca catccactat ttactagaga tggaaatgat
    64441 atttattttg aaatgccaat tacatttgtt caagcaactt taggtgatga gatagaagta
    64501 cctactttgg atggaaaagt taaatatagt gtgccagagg ggacacaaac tggaactgta
    64561 tttagactta aagagaaagg aatccctaga attagaggta attcaagagg agaccaatat
    64621 gttaaggttg tagttgaaat tcctaaaaaa ttaaacgata agcaaaaaga actattgaga
    64681 gaatttgcga aggagtgtgg gtcgaacgtc cacgaaaaga agaaaacctt cggacaaaag
    64741 atagaagata tgtttaagaa aaagtagtta aagtagccaa tatataatca tatttttgaa
    64801 atcttagtta tttataagat tgtcaaagga gatattatga tttttatatt ggctattttt
    64861 gtataaaaaa taaatgtgca taaaataaaa ctgaatataa ataattttct gaaaaatact
    64921 tgtcctaaaa tatataatgt gctataatat aatcaaataa aagaaagggg tgattccagt
    64981 gggaacggat gtggcactgc caaatgatga agaaaacttt attgtaatat acaaaataaa
    65041 attagatact tttagtgcta gattaagtac tggaaaaact catgagctgg gataattatt
    65101 gtaattacct agactcaaac ttttagtagt aaaatagtaa tattatatag acaaggtatc
    65161 taatttttaa atcttttgta tgagttaaaa tttttataaa gggtatatct gattagaatg
    65221 taattaggga aacctagttg tgaattactt ttagtatata catgtgtaaa gaaatatgta
    65281 tatggtattg gttattgtgc tagatgtgta tgtaaaggaa tatgtgtcta gttgggtata
    65341 tgtaaagaaa tatacttata atctatgaaa aatttttagt ataaattgct aaaacattga
    65401 gattgttttt tatagaaagt tagtttatac caactaaaaa tgtgcttaag atttgtttga
    65461 tgtttaatta taaataaggt tgtagataaa aaaataagta aagacaaagg tttaaaatac
    65521 ttgctttaaa tatagtttgt tttgaacact tgccaaatta aaataattaa taaaatagac
    65581 atcttctcat agtaaaatat actgtgagag gatgtttttg ctttgttcat aaacttttat
    65641 gaaaaatttg catagtgtca cattttaata tactatatat ataataaaat atagataaaa
    65701 ttaaatgatt caaaagagaa aggtgaaaag agatgtatta tataggtgta gacataggag
    65761 gaacaggtat acaagcagga gtagttgata actatgggaa gataattttt aggtcagagt
    65821 gtaaaacagt aattgaaaaa ggctttgaag gaatacttaa tgacataaaa ataatgattt
    65881 ataagttgtt ggaagataat aaacttacta tgagtgacat aaaatctatt ggatttggtg
    65941 ttccaggttt tataaataaa gaaggattag taacttgtgt aaatttaaaa tggaataaaa
    66001 aagcttttaa taaagagtta aaaagaagat ttccagatgc tgaaattcat ggagaaaatg
    66061 atgcaacagt agcagcactt ggagaagcaa agtttggaag tatgaaaggt gctaatgttg
    66121 gagtattata tactttagga actggtgtag gtggaggaat agtaattaat caaaaagtct
    66181 tttctggagc acatggattg ggttcagaaa taggtcatca aattatagga gaaaactatt
    66241 ttaattgtaa ttgtggaaat aatggttgtg tagaaacttt ttgttcagca actgcaatta
    66301 taaagtattc tcaaaaactg atagaagaag gagaaaagag taggatttta gatttggcag
    66361 agggaaattt agaaaatgta aatgcaaaga tggtgtttga tgcatataga gaaaatgatt
    66421 tagtggcaat taaagtaata aatagattta aagattattt agcaaaaact tttgcaaaca
    66481 caataaattc tttagaccca gaaattatat ctataggagg aggcatttct aaatctagcg
    66541 atataatact tgatggaata gaagatttgg taagaaaatt tgtattatac aaaacagaag
    66601 atatagctac aattacatgt gcaactttag gttcagacgc aggaattata ggagcagctt
    66661 ttttataatg taaatgaact gcttattgga ggcgtgatga tatatgggta aaacaaatgc
    66721 tagaagaacg ctttttttga tagcaatagg ttcaggaact atgctgaatc ctcttaattc
    66781 ttctatgatt tcacttgctt tacatagtat acaaaatgat tttcatatta gttttacaac
    66841 tgtatcttgg attgtttcag cattttacct agctagtgct attgcacaac cagtttcagg
    66901 taagatagga gatttaatag gtagaaaggt cttatttttg agtggtttga tgcttgtaat
    66961 tttatcagca tccatagtac cacttgttca atcattcttt atattgattt ttataagagt
    67021 aattcaagca attggaacaa gtaccctata tccatctgga gttgctctta tacaaaataa
    67081 tataaaagaa agacaatctt ctgcattagc agttctaacc atttttgctt caactacagc
    67141 aggattaggt ccgacacttg gaggattatt gttggactta ggaggatggc atgctatatt
    67201 tcttgtaaat atacctgttg ttttggtttc tatatgctta gggtattttt tattcccaaa
    67261 agaagtaaaa gaaaagaaat ctataaaaga aactttaaaa aatgttttgt caagattaga
    67321 tattattggc atacttctat tttcaattgc aatgattttt atattactat ttttgctatc
    67381 tatgaaggaa tcttttaact tagaacaact tgtatttggt ataatactga tgtgtctttt
    67441 tatttggcat gaattgagaa caaaattgcc atttatagat attaagcttt ttgtaagtaa
    67501 tcctaaattg tcaaaagttt acttgcaatt tattatatta aatttcttta attatatttt
    67561 gttttttggt cttcctagtt attttcaaga tgccttacat tatagtgcaa agtcgactgg
    67621 attatttatg ctatttatgt ctggtatagg tatatttata tctccattaa ctggaaaatg
    67681 gatagataaa tctggaacta gatttcctct tgttacatca tcaatcttta tgtttattag
    67741 tgcagtactt ctttcagtag tttttatcca tatacctgta ataggaaagg gtattatatt
    67801 atcattagca ggtattagtt atggagttgg taatgtagcc ctgcaatcct ctatgtttga
    67861 agaaagtcct cgtgacagta tagggacagc ttctggtctg tttcaaacta gtcgttattt
    67921 aggttctatt ttagcaactg ttttacttgg aatggtattt gaaggaaata taacatcaga
    67981 acaatttcag atattaggtt atgttatggt ggtcttaggt acaataagtt ttttattaaa
    68041 ttttatatta aagaaagaat taagaaaagt agaatagtgg atttatggat ttaataagtc
    68101 taataactga taagtttaaa aatattgttt gtaaaacata actggtttga tatttattaa
    68161 ttctaagttt tgaagcatag agtactcgat aagtatacaa tattttgaat taaaattaag
    68221 cagaaatttg aactagtctt tcaaatctct gcttaattta gtagttaaat ttttttatag
    68281 agaaaataag atttgacact tagataaaaa atcattgcat tttcaaaatt ctttaaatca
    68341 aacccaagta tctcattaat ctttt
//
LOCUS       DAELSY010000003        57940 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.003, whole genome shotgun sequence.
ACCESSION   DAELSY010000003 DAELSY010000000
VERSION     DAELSY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 57940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57940
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.003"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..843)
                     /locus_tag="KPG09_000168"
     CDS             complement(<1..843)
                     /locus_tag="KPG09_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBG0964797.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGID"
     gene            complement(1224..4559)
                     /locus_tag="KPG09_000169"
     CDS             complement(1224..4559)
                     /locus_tag="KPG09_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG0964798.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEKLRKSLTEKRKVNTVNYDFKIDEVNEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGKYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMVTAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRKLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(4609..5037)
                     /locus_tag="KPG09_000170"
     CDS             complement(4609..5037)
                     /locus_tag="KPG09_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964799.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(5027..5407)
                     /locus_tag="KPG09_000171"
     CDS             complement(5027..5407)
                     /locus_tag="KPG09_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBG0964800.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(5440..5748)
                     /locus_tag="KPG09_000172"
     CDS             complement(5440..5748)
                     /locus_tag="KPG09_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964801.1"
                     /translation="MNQIELDLYHDMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(5781..6965)
                     /locus_tag="KPG09_000173"
     CDS             complement(5781..6965)
                     /locus_tag="KPG09_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBG0964802.1"
                     /translation="MVDEAKILDVVSISRENVKKFDGERSYLSTGNLDFNKISNLEIV
                     TYENKPSRANQTVNIGEVIFAKMKDTKKTLVINKTNKNIIVSTGFYVLKPSKEILPQY
                     LYHYLNSSYFLNQKNRLSKGATQSALNNEGLANIKIRMYNLKVQEKVVRVLDKAQELI
                     DKRKEQIEVLDELVKSRFIEMFGTPSKNEKNWEISEIGKYLDVLTDYHSNGSYETLRD
                     NVTLLDTKGYALMVRTTDLENNNFEKGVKYIDEHAYNYLEKSKVFGGEVIINKIGSAG
                     KVYLMPFLNKPVSLAMNQFMLRFNEDKVNHIFLYNLLLTSYMESKIKEKVRGAVTKTI
                     TKDAVRKINIIVPPIRLQNQFANFVKQVNSLKFEMETSLKELEDNFNSLMQKAFKGEL
                     FN"
     gene            complement(6969..8432)
                     /locus_tag="KPG09_000174"
     CDS             complement(6969..8432)
                     /locus_tag="KPG09_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBG0964803.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFEKIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KESAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVKVLEKEIVDGLEELERMLEV"
     gene            complement(8942..9811)
                     /locus_tag="KPG09_000175"
     CDS             complement(8942..9811)
                     /locus_tag="KPG09_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG0964804.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIGTVKC"
     gene            complement(9831..11747)
                     /locus_tag="KPG09_000176"
     CDS             complement(9831..11747)
                     /locus_tag="KPG09_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBG0964805.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDGHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMISATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(11750..12670)
                     /locus_tag="KPG09_000177"
     CDS             complement(11750..12670)
                     /locus_tag="KPG09_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBG0964806.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(12772..13524)
                     /locus_tag="KPG09_000178"
     CDS             complement(12772..13524)
                     /locus_tag="KPG09_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0964807.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(13821..14438)
                     /locus_tag="KPG09_000179"
     CDS             complement(13821..14438)
                     /locus_tag="KPG09_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964808.1"
                     /translation="MNVIKKFIFVILIINILGCGISLILQSSLGSDAITLLNEGIHIK
                     LGISYTIAGLIYNGTLLLIALLLNKKSLGLGSCVYVLVAGIFIDLYIYLLLDFNISSL
                     GIIIRIMSFIIGHILMCSGFAMLIKFDIGMSPLDAILIYIEGKFKYPYKVLKTIADLI
                     FLILGVYLGGNLGFGTIFSILCTGTTISIVSKMISVNKKRYYSSI"
     gene            complement(14526..15983)
                     /locus_tag="KPG09_000180"
     CDS             complement(14526..15983)
                     /locus_tag="KPG09_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG0964809.1"
                     /translation="MIKLSKFPKDFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKHYESDMTVDKIKFAMKDNNGYYPKRNGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKDKVKYWIVINQINLMYHESFNSVAFCVDQVDDVEEAKYQ
                     AIHHQFMATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRIQYFFT
                     DVSFRGEYPAYIKRFFKENNINIVMEEGDDKVLKENTMDFLALSYYYSTTVSASKNTM
                     NPIDNVKNQYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYKNVIRTNGEELKEKVDSF"
     gene            complement(16005..16358)
                     /locus_tag="KPG09_000181"
     CDS             complement(16005..16358)
                     /locus_tag="KPG09_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0964810.1"
                     /translation="MTTEEHVIDEELVEVAMQIILRAGEARTEIKHALNDLERFDYKN
                     ADLKLAKAKEFMTEAHRAQTNIIQGEASGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(16387..17634)
                     /locus_tag="KPG09_000182"
     CDS             complement(16387..17634)
                     /locus_tag="KPG09_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0964811.1"
                     /translation="MGRLFMNKFIDLLSEKFTPVVNKITKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVALVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDSMLPIAVIMFIGWGLIYGLNFDMFEIIVRIFKPVSDIAQTLPGFV
                     LLNFLVVFLYSLGISGWVLSPITYAIQLSAIAANAAAVASGGLATNIYTYEVVQTGWI
                     TLGGWGATLPLVLMMCLSRAGRIKAIGRACIVPSIMNINEPVMFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYLPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEELKLESQEA"
     gene            complement(17642..17959)
                     /locus_tag="KPG09_000183"
     CDS             complement(17642..17959)
                     /locus_tag="KPG09_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0964812.1"
                     /translation="MKNIKVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLPEIDCLLLGPHLDYMKDEIQKKADEYNVPVACISKTAYGRLDGVKALEEALELI
                     LNK"
     gene            18207..20120
                     /locus_tag="KPG09_000184"
     CDS             18207..20120
                     /locus_tag="KPG09_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0964813.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     STGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNLTLTSAELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDEHFVKLTKKIIKNVYDLYFIDLSDDEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQILENIEIDEDEISYISLHVGSFFDRQKLLED
                     KILCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIS
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIKFMGNILYKNKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(20243..21397)
                     /locus_tag="KPG09_000185"
     CDS             complement(20243..21397)
                     /locus_tag="KPG09_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBG0964814.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(21679..24294)
                     /gene="mngB"
                     /locus_tag="KPG09_000186"
     CDS             complement(21679..24294)
                     /gene="mngB"
                     /locus_tag="KPG09_000186"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG0964815.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNKDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YNRNEIVNAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKISDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIINVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGKKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKIPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANVVFNKNIKTAKWTNLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(24438..26363)
                     /gene="mngA"
                     /locus_tag="KPG09_000187"
     CDS             complement(24438..26363)
                     /gene="mngA"
                     /locus_tag="KPG09_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBG0964816.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDSNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIVLAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKKADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVIGKPVAA
                     LNNGLINWLNGMTGINAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIHVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            complement(26493..27218)
                     /locus_tag="KPG09_000188"
     CDS             complement(26493..27218)
                     /locus_tag="KPG09_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0964817.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(27481..28260)
                     /locus_tag="KPG09_000189"
     CDS             complement(27481..28260)
                     /locus_tag="KPG09_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBG0964818.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(28253..29680)
                     /locus_tag="KPG09_000190"
     CDS             complement(28253..29680)
                     /locus_tag="KPG09_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0964819.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAVSAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGHEVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            30071..30793
                     /locus_tag="KPG09_000191"
     CDS             30071..30793
                     /locus_tag="KPG09_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0964820.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECIGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            30990..32657
                     /locus_tag="KPG09_000192"
     CDS             30990..32657
                     /locus_tag="KPG09_000192"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBG0964821.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNLENEKVREECYKVLEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(32753..34072)
                     /locus_tag="KPG09_000193"
     CDS             complement(32753..34072)
                     /locus_tag="KPG09_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0964822.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYADKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(34113..34859)
                     /locus_tag="KPG09_000194"
     CDS             complement(34113..34859)
                     /locus_tag="KPG09_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG0964823.1"
                     /translation="MKKLVKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(35103..37112)
                     /locus_tag="KPG09_000195"
     CDS             complement(35103..37112)
                     /locus_tag="KPG09_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0964824.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEEIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQSFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQGVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYSITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(37550..38983)
                     /locus_tag="KPG09_000196"
     CDS             complement(37550..38983)
                     /locus_tag="KPG09_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG0964825.1"
                     /translation="MVLRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSREVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMTKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(39070..40986)
                     /locus_tag="KPG09_000197"
     CDS             complement(39070..40986)
                     /locus_tag="KPG09_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0964826.1"
                     /translation="MRRYEQLSKEIIENVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASIGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVIIIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGVLTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTSVVAAMYFKLKDKKIKELCIPAVISGICGVIEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDSIVEISKDNNGQGVVEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTIMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKHFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIIENTQ
                     KHVNNSQELITVLA"
     gene            complement(41060..41920)
                     /locus_tag="KPG09_000198"
     CDS             complement(41060..41920)
                     /locus_tag="KPG09_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0964827.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKCIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(42271..43458)
                     /locus_tag="KPG09_000199"
     CDS             complement(42271..43458)
                     /locus_tag="KPG09_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0964828.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGDNKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIESGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(43472..44863)
                     /locus_tag="KPG09_000200"
     CDS             complement(43472..44863)
                     /locus_tag="KPG09_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBG0964829.1"
                     /translation="MSSLKKKKFTMPQTLTIIFSLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMVFLTIGLYLGF"
     gene            complement(45458..47158)
                     /locus_tag="KPG09_000201"
     CDS             complement(45458..47158)
                     /locus_tag="KPG09_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0964830.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLASNGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(47130..48533)
                     /locus_tag="KPG09_000202"
     CDS             complement(47130..48533)
                     /locus_tag="KPG09_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0964831.1"
                     /translation="MIVNKELIIDYLKSNQENIIKDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGENGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTDDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSSAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(48797..49288)
                     /locus_tag="KPG09_000203"
     CDS             complement(48797..49288)
                     /locus_tag="KPG09_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0964832.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPNEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(49314..50126)
                     /locus_tag="KPG09_000204"
     CDS             complement(49314..50126)
                     /locus_tag="KPG09_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0964833.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLMGYEYKKVLSKEAIKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(50139..51290)
                     /locus_tag="KPG09_000205"
     CDS             complement(50139..51290)
                     /locus_tag="KPG09_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG0964834.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            51560..52201
                     /locus_tag="KPG09_000206"
     CDS             51560..52201
                     /locus_tag="KPG09_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBG0964835.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CTNLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTVDGANTFERDWSKVNK"
     gene            52227..53771
                     /locus_tag="KPG09_000207"
     CDS             52227..53771
                     /locus_tag="KPG09_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0964836.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AVFELATVNKSYTELKEILFSNSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(53988..54614)
                     /locus_tag="KPG09_000208"
     CDS             complement(53988..54614)
                     /locus_tag="KPG09_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0964837.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(54749..55465)
                     /locus_tag="KPG09_000209"
     CDS             complement(54749..55465)
                     /locus_tag="KPG09_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBG0964838.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            55880..57094
                     /locus_tag="KPG09_000210"
     CDS             55880..57094
                     /locus_tag="KPG09_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG0964839.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            57713..>57940
                     /locus_tag="KPG09_000211"
     CDS             57713..>57940
                     /locus_tag="KPG09_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964840.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPL"
BASE COUNT        18879 a        10374 c         6299 g        22388 t
ORIGIN      
        1 atctatacca ttatctgata catacctttt aaaactaaaa tatactttat ttttattcgg
       61 tatacttctc tcctttatag tcaaatagtc tcttataaag ctacttacgt tgctacttgt
      121 atttttttca attttatacc aataattatt ataaaattcc tcttgtaact tagagcttct
      181 acccattaac acaaagtttc ttactctatc agcttcactt aaatctaagc cagttgaatt
      241 tagactttca aagattaatt gagggtcatc ttcaccattt tttaactcta tttctactat
      301 agttaatttc tgtatagcat caaacaaatc atctattgat atattatctt cttttattct
      361 attataaaaa tataaataat ttgaaactat attagaattt ctatctgtat tcatttcttc
      421 ttcaaatagt tcatagtaag cttttctatc ttctgctatt ggttttaact ttatttttcc
      481 atctttatct cttgagtgct tgtttataag ataacactcc ttaatctgtt cttctgttgt
      541 aataattcct tttgattctc caaactcttt tattatttga cacatggcaa tcaataatat
      601 tgacacagta gtcactcttt gttgtccatc tattattaaa tattcttttc ccatactata
      661 tatagataca actgacccta agaaatgagt tctaaatcca gaatctatta tatcctctaa
      721 atcatcaaac aatcttttac attgttcttt cttccagtta taatttcttt gatatacagg
      781 cactacaaaa cttttgtttg acccctctaa ataactaaga aattcaattt ctcctgcttt
      841 catattaact ccccctataa gttaaataaa ttccattcat aactccatgt atctatatat
      901 tatatcaaaa ttttgactca taaatgtata tgaaagcctt attatatacc tttttatacc
      961 tatttttatt ttattaaagt aaatacatga ttattctata aaatatacaa atacttaatt
     1021 ttaaaaatta ttatttaatc ttcttcatga gtaattcata gttatttatc aattaaaaag
     1081 agatattgca taattattca atacctctct ttgtatattt acattatcca tatctagtta
     1141 tctatttcaa cccaatatta gactacctac taataaattt attctctaca taattaacaa
     1201 taattttgca ctaaataaca tcattagcct atctctgatg cattttcttt aattttattt
     1261 atagttttaa tcaatttatt tctctcttca atatgatttt caaataaatc aattatactt
     1321 ccaacagttc taaatgggtc ttccatcaac actttgttat cctctataaa tccattcttg
     1381 actacataat cgactataag tttaacaaaa tgtatttgct tagtattaaa actcttatta
     1441 tttaaaaact ctgaaaaagc ttcatttgca gcttctctat ccaatcccac tagttttctc
     1501 actaattgac ttactggagt atctccaaac tcctttttat aatcatcaca attacctaac
     1561 tgctttaaga gtatttcctc caacgtttca acatcttgct ttgttatctt tttattattt
     1621 cttaatttaa atattgctaa ttcatcttta tggctattta aataatactc taccttcttt
     1681 ttataattct taagattgtt tgcattatat acagaatcat ttctatcttc tgctacaatc
     1741 atatcttcaa aacttgtata atatatcttt tgactagact tttcaagata cttaataagc
     1801 tctcttaatg atatacgtac ttcttctacc tcaaatatat ctgaatcttc ccaaaaatcc
     1861 tctgtcattg ctttttcaat agtatatttt tgttgctgga tctgtggtat agtcccaagt
     1921 ttcgaaagtt tttctgctgt aaccattaca tgccttatcc ctttacttgc attattattt
     1981 tgtaagtatg caagttgtat attatacata actacatcaa atcttttagc caattcatca
     2041 tcactatatg aagttatgag tggtgatata tattctctta tctcattttg tgctactgct
     2101 ccaatattgc tccaaacatt ttcattttta tacttatgta cataacttaa attcatccta
     2161 actatataac tatcctcatt taaagctttt acagaagtta taagttcttc taacaattct
     2221 tttctaagtt ctgcatactt accttctata tatttaatat cttgaagttc tttaaccata
     2281 cttaccttta tattaaataa tttttcagta agtgtttctg ctttatttcc ctcaaatcct
     2341 ttaggattaa atttaaaaaa ttcaaaatta ttacaaaaat caaatattaa gaatctttcc
     2401 ttatccatat caacacctaa taaatctgga cataatctag ttcctctacc tatcatttgc
     2461 caaaacttag tcttacttct cactttcttg aaaaaaacaa gatttaggat ttcaggtatg
     2521 tcaattcctg tatcaagcat gtctactgaa actgctattt gaggtaattt attcttatca
     2581 gaaaaatcat ctattataga atctacataa tttatactat agtcaactgc ttttacaaag
     2641 ttcccaccat actttggata cagtttatta aatctttcaa ctattgccct agcatgcttt
     2701 gtattctttg caaaaattat agtcttacca atcttttctt ctccttctat tttcaacccc
     2761 tctgtcatta gcttatttaa aactaaatct attgtgtcag aattaaatag ccactcattt
     2821 actgcattat tccctatttc gtctccaata ctctcatcat cattaaatgt attttcatat
     2881 tcttccttct cttcatcaga aagctcatca tacttaattc catcttccat aatcttagtc
     2941 ttaaactcaa tactagtata atctactaaa tatccatcct cgactgcttt attatactca
     3001 taagcatatg taggtactcc attttccatg tcaaaaactg aataagtatt tttatctatc
     3061 tcatcctttg gtgtagcagt aagacccatt aagtaagaat caaaataatc aaagattgac
     3121 ttatactttt tataaatact tctatgcgat tcatctatta taattaaatc aaaatgacca
     3181 ggtgtaaaca atctctttcc atccttagac ttcgtatcat caattgcatt catcattgtt
     3241 ggatatgtag aaaatatcat cctagcatct tctgggctgt ctttgctatc caataaatta
     3301 cataacgcca agttaggtaa tagcttagta aaactttttt tagcttgctt taccaatgct
     3361 tttctatctg ctagaaataa tatattttta atccaattat gtcttgaaag tacatcaact
     3421 atagatatag caattcttgt ttttcctgta ccagtagcac ataccaaaag agttttgcgt
     3481 tgcttatttt caagagcctc acatacagcc ctaatagctt ctttttgata atatctattg
     3541 gatatttcat catttattgt tacagaactt aaagacttct tactctttct tctatctatc
     3601 ataagttgta attctgattt tttataaaat ccataaactt ttctatctga atagtcatcc
     3661 caaatataca tatcaaaacc attcgtcaaa aatattactg gtctttgact atatctcttt
     3721 tccaaacaat ctgcatataa tttagcttgt tgttgaccaa ttttcgggtc tttgctcgtt
     3781 cttttcgcct caactactgc taatggctta ccattttcac cataaagaac ataatctaca
     3841 tatcctacac cactttcatt aggcatacct tgtacttcta tttcctcccc tatatctttt
     3901 ccaaattccc aacctgcaag ctttaattct atatttatat atctttttct tgtctcaaat
     3961 tcgtttactt cgtctatctt gaaatcgtag tttacagtat taacttttct cttttcagtt
     4021 agtgactttc ttaatttctc attttctttt attacttctt caagcttttt atcttttgaa
     4081 cttaatctct cataaagatt tttaagctcg tctggtctcg tccttttctc ttccccatga
     4141 agtaataaat cttcattaaa gtcttctgct gtatattcat ctgaataaca atagtctatc
     4201 caactaacaa attgatgcaa attgttaagt gaaagtattg cttctcctct ctctatgttc
     4261 gcactagtat gtacagataa attacctaat ttaactatat attttaataa aggtaacata
     4321 tcatagtcta taagttctat aaaactatta ttatgaatca acgtactaag attatcttga
     4381 taaggcaata ctaaatcact atcattagca tatagccatt ttactgcaag ctctaaagct
     4441 cttcttgtta tagttgcaca agtagatggg ctaactagta tacttttttc tgcttctaag
     4501 caagcagtat taaaactatt gaaatttttc ttcttttgta aaaagtcaaa attacccaag
     4561 gcttttctcc cccttttgtc ccttagttaa aataaatctc tctaaaaact atttcttaca
     4621 gctgtttctt atactaacaa atacttcttc attttctgta aacaattcgt acatatcttc
     4681 aaactgtggt atatcatatc catgagaatc atcatttctc aattttctaa gtgtttctat
     4741 aactatagaa gaatttttag gtaataaatt gtgatactca caagctaaaa ggtagtcata
     4801 aaagttttta gattttatgt ttaaatcaat tgatttataa tacaacattg cttttgttaa
     4861 ttctaaaaat gagaccatat agcttttaaa accttctttt atgtatctag cccataactc
     4921 aggcatttct tccttatcat ataagatttt gaagttttgc aggtcatata agcaagaacc
     4981 aaaatcccta tttacttctt ctattgagtt ccaagataat ttgtttctaa ttaccataca
     5041 tgtccctcca atctaagata tctattacat cactgttttc aaaatagaat ttatttatat
     5101 aatccttaaa atctttagat tcataaataa cttcaccttc taaaatagct tttttaagtg
     5161 ctattgtata attatctgta acaattttta ggtctacatc aatgtctaat atatttctta
     5221 gagcttctgt cttagttgca agttctgtaa ttggtacttt tttgatagga atccaagcta
     5281 tgtctatatc gctttctgat gtatgtttat ctgttccata actaccaaat attatgattg
     5341 ctagagtatt ttcttttgga aatacattta ttattgagtt tttaatctca tttggtatat
     5401 ttctcattta atcacctctt gtttattagg taatattgct taattgaatt tatatccata
     5461 atcttttaat ctatttatac atatttctct ttcttcatca gtaaacaact ctttatactt
     5521 ttcttttaat actaatactt ctaatgataa atctaatctc ttacactccc atagccttgt
     5581 aaatccttca gtagctcctt ctttattgat taattcttta gcagctaata aggctccttt
     5641 gttatttatc agttgtaaaa atcttgttgg cttatagtta cattgtaaat cagcttcttt
     5701 atagatattt accatgtcat gatataaatc tagctctatt tgattcatag attttattcc
     5761 cccattttaa tacttgtatt tcagttgaac aattctcctt taaaagcttt ttgcattaat
     5821 gagttaaagt tatcttctag ttcttttaag ctagtttcca tttcaaactt caaagagttt
     5881 acttgtttaa cgaagttggc aaattgattt tgaagtctaa taggaggtac aataatattt
     5941 atttttctaa cagcatcttt agttatcgtt tttgttacag ctcctcttac tttctctttt
     6001 attttacttt ccatatagga tgttagtaaa aggttatata agaatatatg attcacttta
     6061 tcttcattaa atctaagcat aaattgattc atagctaaag atactggttt atttaaaaat
     6121 ggcattaaat aaactttacc tgcactacct atcttattta ttataacctc accaccaaac
     6181 acctttgatt tttcaagata attatatgca tgttcatcga tatacttaac acctttttca
     6241 aagttattat tttctaaatc tgtagttctt accattaagg cataaccttt tgtatctaaa
     6301 agagttacat tatctcttaa ggtttcataa ctaccatttg aatgataatc agttaaaaca
     6361 tctaaatatt tccctatttc tgaaatttcc cagttttttt cgttctttga tggggtacca
     6421 aacatctcaa taaatcttga tttaactaat tcatccaata cttctatttg ctcttttctt
     6481 ttatctatta actcttgagc cttgtctaaa acacgcacta ccttttcttg cacttttaaa
     6541 ttatacatgc gtatttttat attagctaat ccttcattat ttaaagctga ttgtgtagca
     6601 cctttactta acctattttt ttgatttaga aaataactac tatttaagta atggtataaa
     6661 tattgaggca atatttcttt tgatggtttt aaaacataaa agccagtcga tactattata
     6721 tttttattag ttttattaat aactagtgtt ttctttgtgt ccttcatttt tgcaaatata
     6781 acttctccta tatttacagt ttgatttgct ctagaaggtt tattttcata agtaactatt
     6841 tctagattgc ttatcttgtt aaaatctaag tttccagtac taagatatga gcgttctcca
     6901 tcaaactttt ttacattttc cctgcttata ctaacaacat ctaaaatttt tgcttcatca
     6961 accatactct acacctctaa cattctttct agttcctcta atccatccac tatctctttc
     7021 tctaacacct taaccctacc tataataaca ctaggagcat cataaactac ttcctcatac
     7081 actacctctt tatacttatt aatagataaa tcatacccat tctctcttat ctcttccaca
     7141 tcaacaaaaa agctttgctc tgttcttttt ctatctactt ccccatcaag attatcaaat
     7201 ctcttaataa tatcctctat atcactatcc tttatctcat ttctcttatc atccaaagaa
     7261 taaccatcag ctttcatatc atagaaccat accttatcag ttccacctgt ccctgtttta
     7321 gtaaatatca ttatagctgt agatacccct gcatatggct taaatacacc acttggcatt
     7381 gatattatag cttccaattt atgattatca actatttctt ttcttatcga tttatgtcca
     7441 cctgttgaac caaacaatac tccatctggc actatacatg cgcatcttcc accaattttt
     7501 aaaactctta aaaaaagtgc caagaataat aactctgttt tctttgtctt agtcactttt
     7561 aataaatctg ctgacactgc ttcataatct aaacttccct taaaaggagg atttgctagc
     7621 accaatgtaa acttatcttt atctgtatta acctccgata aagaatcctt atactctata
     7681 ttaggattgt caacgccatg caacatcata ttcatagcac ctattctaag catagttcta
     7741 tccatatcaa atccatagaa cataccatta ttaaaatgct ccttcagccc ttgcactaag
     7801 aaaagacttg aatgattatc ccttaagtac tgttgtgatg atactaaaaa acctgccgaa
     7861 cctgctgccg ggtctactat aatatcttct ggtgttggtt taataagtct tactatcata
     7921 tctattatgt gtcttggagt cctaaattga ccattagtac ctgctgtagc cactttggat
     7981 aataaatact catacaaatc tccctttgta tctctatcct tacttaattc taaattacta
     8041 ataccatcaa ctattttaga taacataaga ggcgtaggaa ttttaaatat tgcatctccc
     8101 atgtactttg cataagctga ttctttattt ccatgtaatt tttttatgaa aggaaacact
     8161 tcattttgta ctatttcata cattttttct gagtttcctt cattagaaaa cttactccat
     8221 cttaaatgtt gatgttcatc atcaaatatc ttttcaaaag aaacacccaa aagagttgct
     8281 tctgcttcat tttttgtttc tacttcatcc aatcctttaa taaataaaag atatgtaaat
     8341 tgttctataa cttctaatgg attagttaat ccaccagtcc aaaatgtctc ccaaatcttg
     8401 tctactttac tccttaattc tcctgtaatc atatatgact ttccctccag tgttcataaa
     8461 ataatttaaa tatatatttt agtcgtacgc cttaatagtt agtgtcttat gttaaatcca
     8521 agtttttact acacaattat taaattattt ttcccattta catattttag ctaaaaaact
     8581 aatgcaatac tcttatttta tgattattat atataccata caaaattatt ttatcatata
     8641 tccatcatag aagtcatcta cctggataac tcttaccata ttataacttt ttaatattaa
     8701 tttaaactta gaaagaaatt ttttatttaa ttatatcatc ttccttattt caaatttctc
     8761 aactacaatc ctaatcaaaa aataaaataa gatgctgtct caaaatagat ataatcttct
     8821 tttttattgt cagaatgatg aaaatatatt tgatttcaca caaaaaagag tgcctcatga
     8881 actaaaaagt tcattttgag acacccctta atttaatctt acttattctt tccctttatt
     8941 attaacactt cactgtccct attgcttttc ctacacatcc acaaacttcc atcctactct
     9001 taacaaaatc tttaactgac tctaaacctt ttttaccata tttcttaggg tcaattacct
     9061 ctgggtcaga attaagcaca tccttaaccc cattgctata agcaatcctt aattcagttg
     9121 cataattaac tttgcaaata cctcttttta tagattcctt aataacctca tctggtattc
     9181 cagacccacc atgtaatact aatggaactg atacaacctc tctaatttca gataatctat
     9241 ctaaatcaag ctttggctct cccttatata gtccatgtgc cgtacctatt gctactgcca
     9301 aagatgatac ccctgttctc tctacaaact cttttgcttc taatgggtct gtatatgctc
     9361 caccatcacc accatctaaa tcgtcttcct tacctccaac tttgcctaat tcagcctcaa
     9421 caggtatatc tgaaggcata catgcatcta caacagattt agatattgaa atattatctt
     9481 cgtaactttc atgagaacca tcaatcataa tagaagtata tccaactcta agtgcctgca
     9541 tagctagtcc aaagcttgaa ccatggtcta agtgcattgc cactggaaca cttgccttct
     9601 ctgatgctac ctttacattc gctaaataat aatctagccc tgcatacttt attgttgaag
     9661 gtgttgtttg caatattact ggtgatttaa gttcttcagc agcctctatt actgccataa
     9721 ccatctccat attttcaaca ttaaatgcac ctactgcata atgcccttct tgtgcatcta
     9781 ataatatttg tttagttgtc actaatgcca taatttctcc tcctatatgt ttatactgat
     9841 ttgatttctt cgttatttaa ttttgatact atttctctac ttgttttgct atttaataac
     9901 tcatttaaaa attcttcatt cattaatttt gaagatagtt gagataatgt ttttaagtgt
     9961 aaattatcac cattatctgt agctgatatc ataaatataa tatgtgccaa gcttccatct
    10021 tctgattcaa agtcaaaccc ttcttttgat acacctactg ctacacgtgg tacctttact
    10081 gcatctgttt ttgcatgtgg tatcgctatc cccataccaa aacctgtaga tgatatttct
    10141 tctcttttaa ggatttcttc tttaaacttc tttttatcta aaagaacacc agatttatca
    10201 agtattccaa tcatttcatc aattacatct gttttatttt ttcccttaac atctaaaatt
    10261 actgtttctt cgctatataa agagatatta ttagatacgt tttccgattc tgcactttca
    10321 tttttatgtc catcttttgt tgcatttgta ttttctatag tatttttctc atttactact
    10381 ttaggtgtaa ccatcatata aagtacagca cctactaatg aacctacaaa tatacaccct
    10441 acccataaaa gtggcttatt gactaatcct agaaccaaaa accctccatg tggagctggt
    10501 acttctattt tcattatata agttaaaatt gatgatattg atgatgcaat cataagtact
    10561 ggcaacactt ttaatggatt ttttgctgca aaaggtattg ccccctctgt tatatgagta
    10621 ctacctagta tataattgat taatcctgct gctctatctt cttctgtaaa tctattttta
    10681 aataaagtag atgctattgc tattaccaat ggtggtgtta tacaagctgc agatacacct
    10741 gccataaata aataatttcc ctgtcctaga agcatagttc cagttacata tgctgcttta
    10801 tttactggac ctcccatatc aaatgctgac atacatccta ttactattcc caaaattata
    10861 ggacttgaat tttgtaaccc cgatagaaag ttcatcatag cattgttaag tgctgaacat
    10921 ggtccaccaa gtaacaacat tgctacaccc acaagaccta cgctgacaat aggaattata
    10981 aaaatcgatt taagaccttc taattgtcgt ggtaattttc ttaacaaatg tacaaatagt
    11041 ttaactaagt aacctgctaa aaatcctgat atgattccac ctaaaaaccc cgaacctgta
    11101 tcaaatgata tcattcctcc aacaagtcct gccaacatac cagctcttcc cgatatagat
    11161 tgagctataa atgctgccaa tacaggaacc atcaagttca tagaatatcc accaacagat
    11221 tttaatgtag ctgctatttg attatattgt tctgaatttg ggtcaaatga ataaatcccc
    11281 cataaaaatg atatagcagt aagtactcca cctgctacta ctaaaggtaa catgtgagat
    11341 acaccattca ttaagtcttt atacaacttt tgaccaaacg ataaatttcc ttcttctgca
    11401 attttactgc ttgataagtt tccttttcta atagctactt ttccatcaat acacctttgt
    11461 ataagttcat tagccttatg tataccttct ttgacaggta cttctattac cggctttcca
    11521 ttgaatctgt ccatatcaac atttttatca caagcaacta ttattgcatc tgccatttcg
    11581 atatcttttg ctgttaactt attttctaca cctatagctc cattcgtctc aacttttatg
    11641 tcacacccta attcccttgc tgaatttttt aatgcttctt ctgccataaa agtatgagct
    11701 attccagttg gacaacctgt tacccctaga atcattttct ttcccatctt tataccacct
    11761 ttctgactct tatatttttt atataatctt ccacattctc caaattacct ataccattac
    11821 tttcagccac acttgctcct gttgctgctg acctttttaa tgcttcttca atatgttctg
    11881 gattttccaa ccatatactt aaaaaagcag ctaatgcaga atcaccagca catgcagagc
    11941 tgactactgt aactggttga gcactagcat aataaattga ttttccatta taaaaataag
    12001 aacctttttc tcctaaagtt agcaatatat tttgagcacc ttttaaatgc agtaatttaa
    12061 gtacatcctt aatatcttct tcatctctta caataattcc aaatatatct ttgatttctt
    12121 catcatttgg ttttatcaaa tatggattat actgtagtaa ctcctttaat tttggtgaac
    12181 ttatatcgag tattgtcttt atacttttat ttttacaaat tttaaatata tcctcatagt
    12241 aattatcatc aataccagga ggtagacttc cacttattga taagtaactt atatctgcta
    12301 atgactgtat cttatctagc atatctagtt tttgtttttc gtttacatat gacccagagt
    12361 ttacaaattt aaattcatca cttccatcat ttaaaaatat atttatacga gttgtatctt
    12421 ctacccaagt aggtaatacg tcaaatcctc tcttttcgct ttcttcaact atatattttc
    12481 ctgaaaatcc tccaaaaaat cctaatatct tagattctat accataatga cctagtacaa
    12541 aagttacatt taatcccttt ccattaggag aatatacagc atttgatgta cgatttacta
    12601 attttctctt tatcccatta gtagatatat tcatatctat tgcaggattc gtagttaatg
    12661 tatatatcat tgtcttcccc cttgcttgtg ctgtgtatgt tttcattata ataaaaaagt
    12721 ctatgaaaat cttttcacaa atagaaaatc gattttcata gacttttcaa tttaattttc
    12781 agaattatta tatctgacca tataatctat aattatccta gatataatat acaagggcaa
    12841 tctggaagat acttcatatt cagaaattga tatatgttca tgtttgtatc ctacaatata
    12901 taaatccgaa tactttataa gttttgcact ttcactacaa cagcaagtta taaccttagc
    12961 tccaactaaa ttggcattct gtgctgattc tacaagctct atggtatttc cattcaatga
    13021 aaatatgatt ataagttcgt ctttttttat tgatttagat tttattttca ttatgttggg
    13081 gtcatttata aaaacaacat taaaatcaag taattgcaat ttaagactaa actcttttgc
    13141 tacatattca gtaagccctc ttgccaaaat atatatctta ctagatttat ttatagtttt
    13201 tattatattg attagtgtag ttaaagatat ttgttctata actctctgtg cttctataaa
    13261 ggatttattt attatatccc ccatattttg tatatcatca ttttcatctt ttactgttaa
    13321 tctataccta agttcattga atccatttaa accacatttt ctaattgctc ttgaaacggt
    13381 tgctggagat gaatatgttt catgagcaat ctctactata gataattctg gtaatattga
    13441 ttcattttca tttataaatt taattatttc aagttccgtc ttcgttagat tttcagaatt
    13501 acttttgttt agctcaatta tcatatgtta tctcccctta tataagtttt atttgtctaa
    13561 atttatcgag taatataata tattacctct taactactta tttttatata caattaagat
    13621 ttagtcaatg tctatatatg taaatttata atataataat tttatgcttc aattaattat
    13681 gtaattatta gagtattttg taataaaaat tggagttact caattactga aactgagtaa
    13741 ctccaattct tacagcctca aattaactcg aattattgaa aatgattcat aaacattatc
    13801 ctatactctc acttatcaat ttatatactt gagtaatacc tttttttatt aacagaaatc
    13861 atcttactaa caattgaaat tgtagttcca gtacataata ttgaaaatat agttccaaac
    13921 cctaaatttc cgcctaaata tacacctaaa attaaaaata ttaaatctgc tatagttttt
    13981 aaaactttat atggatactt aaatttgccc tcaatataga ttaatatagc atctaaaggg
    14041 ctcattccaa tgtcaaattt tataagcatt gcaaatccac tacacataag tatatgtcca
    14101 attataaagc tcattattct aatgataatc cctaaactac ttatattaaa atctaataat
    14161 aaatatatat acaaatctat aaatattcct gctactaata cataaacaca gcttcctaat
    14221 cccaaacttt ttttattcaa taacaatgct ataagtaata gagttccatt atatattagc
    14281 cctgctattg tataacttat acctaacttt atatgtatac cctcatttaa aagtgtaatt
    14341 gcatcactac caaggcttga ctgaagtata agagatatac cacatcccag tatatttata
    14401 attaaaatta caaatataaa tttttttatg acattcataa ttaataaacc accttattta
    14461 taattataat ccctcttata aaactgtatt aataacatag tatttataag agggattatt
    14521 tatatttaaa aactatctac tttttctttt aattcttctc cattagttct tattacattt
    14581 ttataccaat aaaaactatc ttttttgtaa cgttctttgc ttccatttcc attgttatct
    14641 atatctacat atataaatcc atatcttttt tccatttctt gagatgaaca acttactata
    14701 tctattggcg accacataca ataagctaat atttcggttc catcagctat agcttcacct
    14761 atttgttcta tatgttttcc taaataatct attctataac tatcatgaac ttttccatct
    14821 tcaaatttat catgcattcc aaatccattt tcagctatca ttattggctt ttgatatcta
    14881 tcataatatt gagataaagt attgtataat cctttagggt caattgccca cccccaagga
    14941 tttgctttta agtattgatt ttttacatta tcaattggat tcatggtatt tttacttgct
    15001 gatactgttg tagagtaata gtatgataga gctagaaaat ccatagtatt ctcttttaac
    15061 actttatcat caccttcttc cataactata tttatgttat tttctttgaa gaatctctta
    15121 atataagctg gatactcccc tctaaaactt acatcagtaa aaaaatattg tattctattt
    15181 ctttttaaag caaatactac attttcaggg tcgcaatctt ctggataaga tgtacaatct
    15241 gccatcatca tccctatcat catatctgaa tttatttctt ttccaagctt cttagttaaa
    15301 gctgtagcca tgaattgatg atgaatagct tgatatttgg cctcttctac atcatctacc
    15361 tggtctacac aaaatgcaac tgaattaaat gattcatgat acattaaatt tatttggttt
    15421 ataactatcc aatatttaac tttatctttg tatctattta atattaattc tgcatatcta
    15481 acaaagaaat ccactacttt tctattagac catcctttat attttaatac taaattcaaa
    15541 ggagtctcat aatggagtag tgttacaata ggttccatac cattatctat tacttcattt
    15601 ataagattat cataaaactt taatccctct tcattaggct ctaaatcatc tccatttgga
    15661 aatattctag cccaatttat tgaaaatcta aaacacttta accctagttc tttcaaaagc
    15721 tttaaatctt ctttgtatgt atgataaaag tctattccat tacgcttagg ataataccca
    15781 ttattatctt tcatagcaaa ctttatttta tctacagtca tatcagactc gtaatgttta
    15841 gatatatctc tatctttatc atacatatgt acatcagcta agcaccaccc tttgccacct
    15901 tcattccaag caccttctaa ttgattagct gctacagctc caccccacaa aaaatcttta
    15961 ggaaacttac tcaatttaat cactccttat tttatttatt taaatcattt actattattt
    16021 accttatatt ctatagactt tactattcct actaaagaca tagttatatt taactcgctg
    16081 aatattgtca taagagtatc ttgagcatgt gcaaataaaa gtgaatgtgc tcttttttct
    16141 ccactagctt ccccttgtat aatgttagtt tgagctctat gtgcttctgt cataaactct
    16201 tttgctttag ctagttttaa atcagcattt ttataatcaa acctttctaa atcattaaga
    16261 gcatgtttaa tttctgttct agcctcacct gcacgaagta ttatttgcat tgctacttct
    16321 actaattctt catctattac atgttcttca gttgtcatat ttataatttc ctttcaacat
    16381 ctaaatttaa gcttcttgac tctctaattt tagttcttct ttttcataag ctttaaaaaa
    16441 tggataccat atcatgaata gcaatactat atttattgct aaaacaataa gccctctaac
    16501 atcttgattt accataaaag tagatatacc aaaaggaaga taccataaat taaacacctt
    16561 actcggaatt gtcacaaatc ccatactcat tgataagtaa gtagttattg atattactac
    16621 tgttcctatc cacataggta acattaaaaa tgggttccat gctactggag ctccaaacat
    16681 tactggttca tttatgttca ttatagaagg aacgatacag gctcttccga ttgcttttat
    16741 tcttccagct ctagataaac acatcattag cactagtggt aatgttgctc cccacccacc
    16801 taaagttatc caacctgttt gaacaacttc ataagtatat atattagttg caagccctcc
    16861 tgatgctaca gctgctgcat tagctgcaat tgcactaagc tgaattgcat aagttattgg
    16921 ggataatacc catccactta tacctaaaga atataaaaat actactaaga agtttaatag
    16981 tacaaatcca ggcaatgttt gtgctatatc tgatactggt ttaaatatcc taactattat
    17041 ttcaaacata tcaaagttaa gtccataaat tagtccccaa cctataaaca ttattactgc
    17101 tataggtaac atactatcaa accacttact tacaaaatca ggaagtgctg aatcttcacc
    17161 aaaaaatgag aaagacccaa ataaagacat tattaatccg gagaataatc ctgcaactag
    17221 tgctacaagc attccttctc ctccaaatct attgaagtta aatatagctc cttcattcgt
    17281 aatctctgga cgaatcatca ttaacattaa tccagcacct gcaagaccag ctactacact
    17341 catactttca aatttcttat tcttcattat ttccataggt aataaaaatg ctataaatag
    17401 tcctaaaagt ccaaatgtat attgatttat tggacttaaa tcaggtaaga aacttatgta
    17461 attcttcaag acagaaacta tagttactaa agaacctaca aataccatag gcaaaacttt
    17521 cattatagtt gattgaactg attgtaccca tatattctta gttatcttat ttacaacagg
    17581 agtaaacttt tcacttaaca aatctataaa tttattcata aacaatctcc ccatagatat
    17641 tttatttgtt taatattaat tctaatgctt cttctaaagc ctttacacca tctaatcttc
    17701 catatgctgt tttagatata catgctactg gaacattata ttcatcagct tttttttgta
    17761 tttcatcttt catataatct aaatgtggtc ctaataataa acaatctatt tctggtaaat
    17821 atttatctat ctctgactca cttctagctt ttatatctgc ttctatgcct tttttctttg
    17881 cactctttct catactagtt gccataaaac ctgtacttgc tcctgagcca catgccaaca
    17941 acacttttat attcttcata acaatacctc cagtattttt attcattaat ttatttctta
    18001 atgaattttt atttgatttg attgtattta tattgtactt cctaaataat ttcctttcac
    18061 ataaatatat ttcctaataa taggaaatca tttactctta accagacaca taaatatata
    18121 gtataatgta cattataata cgtacattca attatataaa tatcatatca tacagatttt
    18181 attttaaaac tatgagaggg aatctaatga aaacatctaa aaaagagtta tttttaaact
    18241 tcataaaaac gcaacatgat tggatagatt catccacatt agctaattat cttaatgtat
    18301 caacacgttc gattagaaaa tatgtaaatg aaattaattc tactggtgaa tttatattat
    18361 cctctaaaaa aggatataaa gtaaatctca ataataactg tcaaaccaag gttgatagtt
    18421 cagaaaatat atcacctgat aacaggttaa atttaatttt aaaagaactt atagttaata
    18481 gtaatggaat taatatattt gatttaagtg aagaattgtt tgttagtcca gctactattg
    18541 aaggggatat agtcaaagct aataaattta taggaagtta taatttaaag ataaagcaat
    18601 cgaaattttt attgaaacta attggaaatg agtctgcaaa gcgcaaatta atgagctcta
    18661 taatatttaa agaaacggga agtgattttt taagcttatt tgatgtacaa aaaatatatc
    18721 aagagtataa tttgaccaaa ctgaaagaaa atataatata tatattaaaa aaatacaatt
    18781 tgtttataaa tgagtacgct ataaataaca tacttcttca tttaatgata actatagaca
    18841 gaataaaaaa gaataactac atagaatctg tcgaagtagt aaactacata gataataata
    18901 atgatataag catttcaaga gatatatcaa attttttaga gtctgaatac aatttaactt
    18961 taactagtgc agaattatac tacttagtat ttcagttaac aaacaaaacc acagtcttaa
    19021 attatcatga aatggatact aagtcattgc caaattatat agatgaacat tttgttaaat
    19081 taactaaaaa aataattaaa aatgtatatg acttatactt tatagattta tctgatgatg
    19141 aatttgttgt aaaatttact cttcatgtaa aaaatttaat cagtagggct aaaaataatc
    19201 aagttttgag aaatcaaata cctcagaagt taaaagattc ctaccctctg atatatgata
    19261 tatctgttta tatttgtaat caaattcaaa ttttagaaaa catagaaata gatgaagatg
    19321 aaatatcgta tatatctctt catgtaggtt cattttttga tagacaaaaa ttactagaag
    19381 ataaaattct ctgtgcatta attactccaa attactatga tttacaattc aaaatagtaa
    19441 gagacctcga gaagagattt aatgaatcca tagaaattat tcaaattttt tcagatactc
    19501 ataatcttga ttttgacaat aaggtagaca tggttattac tactttacca attagtgata
    19561 gatgccctat accttttgtt tatgtaaatc cttacctaaa cagaaaagat tatgataata
    19621 tccaaagtaa atttattcag attaaagata gaaaaaatat acttactgta caaaatcatt
    19681 tagaaatgta ctttagtgaa tctttgttta tgaaaaacat atatttagat agtgctaaag
    19741 attacataaa atttatggga aatattctgt acaaaaataa atatgtcaaa cccaattata
    19801 tagatgatgt tcttataaga gaaaaaatgt cttcaactgc atttaataac aatgtagcaa
    19861 ttcctcatag catgaaaatg gatgctttaa aaactggagt atgtttaatt gttaacgata
    19921 aaccagttaa atggggtgag gaaaaagttc aaattatagc tatgatacct ataaatgaaa
    19981 aagaaaaaga aaaattcaat tacatatttg aaagctttat tgaaatatta tctgagtgga
    20041 ataatgttaa agaacttact aaggcagata actacagtag ttttatgaat agaatagctt
    20101 atcttattca aaatatctaa atccattaat ttatttccaa tatatgtttc ttaagttatt
    20161 aacacaaaat agataagctg tcacactaaa aagtctaaat taatatgtga cagcttttta
    20221 cctaatatac tttgctttaa tactacatat tttttgattg ttgtatacta gttataagat
    20281 tgaatatttg tgttgttgta gctgtcatat tctcactagc agttgcttta tccattgctt
    20341 cttctattgt catagctcta tttactatag aaaaataagc atctataccc tcttcattac
    20401 atttaactgc atcttctgtt gtggctccag ccaaacctat tacttttacc ccatattttt
    20461 ttgccagttt tgcaactcct attggagcct tgcccatagc agtttgatta tccaatctac
    20521 cttcaccagt tatgacaaaa tctgcatctt ttaactcttc ctctaattta atttcttcta
    20581 atattatttt tattccagat tctaattttg aatttaaaaa tgctaaaaaa gcaaaaccta
    20641 atcctcctgc tgctcctgct ccttctacat tagctacatc tttacttaag taatttttaa
    20701 ctactttgga aaaatttcta agcccattat ctaactcctc aattatttct gatgttgcac
    20761 ctttttgaga cccatatatg tatgcagctc catttttccc aaacagcgga ttatttacat
    20821 cacaggctat tttaaactta cattcatcta attcctttaa tctattttct atttttatat
    20881 gtcttatttc atttaaaact tttccaccta atcctactaa cttgttattt tcatcataaa
    20941 actcaaaccc taaggcttga agcataccta tcccagcatc attagtagca cttccaccta
    21001 ttccaactat aaaatttcta tagcctttat ttattgcttc ctttattatt tccccaacac
    21061 cataagttgt agtgtataaa ggatttctaa gttcagtcgg aaccaatgtt aatcctgatg
    21121 cttgagctat ttctattatt gctgtacttg tattttttaa taatccatat gttgcatcca
    21181 cttttttagc tattggtcca gtaacttcta tagtctcttt tttaccattc atcccttgaa
    21241 ctaaagcatc tactgtccct tcaccaccat cagcaagtgg caatatcttt acttgcgcat
    21301 cttctttaac cttcaatatc ccctttttta ttgcatttcc tgcctctatt gaagataaac
    21361 ttcctttcaa agaatctata gaaattaata ttttcatatt tatctactcc tttttattta
    21421 ctctttattt taaatatata attttataag cttaatttat cttttatttt atactctata
    21481 tttcactttg tatacaactt atacccttaa tattctttta tcttataaaa aagggagtgt
    21541 ctcaaaatga actttttagt tcatgagaca ctctttttgt ataaaattaa atatatgttt
    21601 attatttcaa caatgataag gaggattatc tctattttaa gataattcct ttttagcact
    21661 atctaatcat ttttagcact atttaaccaa tatagtttta acttggttca ttaggcactc
    21721 tacatttaca gagttattat ctattgatat agattctaat tctttttcat ttaggttagt
    21781 ccatttagct gttttaatat ttttgttaaa tactacgtta gctgattttg tttcttcttt
    21841 agaattgtaa aatctaacta ctaatgcctc ttctttttca gcctttttta caacacttaa
    21901 cacaaggtcc ttattagttt ccttaagtaa actatatgaa taatctactt ctatttctga
    21961 ttgattcaat ttcatagcat tatatggtat tttattataa gtttgtattt ttgttaaata
    22021 ctctttagcc atatttgcaa catttgcttc tagtgtagat ttttcatgag tgactattgc
    22081 taagtctatt tttatatctc ctatcatttg agaatctgga gtaggcaact ttattcctga
    22141 tggacgacct gggcgtcttg ccatttcttc tttacctagg aatcctacac ttctaaataa
    22201 tgttatagct attgtgtcaa attttttccc tactatttca aactctcttg tactgtttgt
    22261 aagaacagct actccatgct ctttgtcaga caatccaacg aatgttagca ttggatatat
    22321 agaatctggt ctttcactcc attcttcctg ttgccaaact tccatagcat catcatacac
    22381 acttctcttt atatgtccaa actgattatc agacacagaa aactttgaag ctatatttgt
    22441 tggtatataa gttctaaccc tacaatctct tgcttggtta tttatattga atactacatt
    22501 tattatagct ttatcatttg gaacatttat aactatatca aagtcaataa atgaatctgc
    22561 tctattattt tttctagctt gtatatcttt tggtactttt aatctataat ttatctttat
    22621 ctcattatta aatctatttt tctttatagt ggaatttgct actatattat cgttaaatat
    22681 taatttctca tttggaagtg gtgaaaaatc atactcatct ccttcatctc ctccattttc
    22741 catcaataat acattttcaa aagttttatt taatttttta tcagatatct ttaatgtccc
    22801 gttttcatta acttgaatag tataaaagtc attatctacc atgtcacata cttcataagc
    22861 tttttctaac atatgttcaa cttcttttat catatacgcc ttatacccca ttgcaggtat
    22921 tttatcttta aagtttattg tatataccat aaatgaatca tagtttccat aatgaacaat
    22981 ttgacggtct attaatcctg catctattac ttctttatgt accacttcaa aatccaactt
    23041 attattatct ttatcaaata tttcaaatgc tttcatttta gttataactt gggcgttcac
    23101 tatttcattc ctattgtaag gcattaagtt aaatgctact aacttatcta aagccatttc
    23161 acaactcatt gcatcaacta ttcttctttt gtaaaatgtt attaattggt ctactttttc
    23221 ctcagctaag aaaaatctat tcattatttc ctggtgaact ttatctgaac aacaacatcc
    23281 aatgctgtca tgagcatgat ttttcattat ttctttccaa attaactcta ttaatccatg
    23341 atgatattca aatcctagag aatatgcaat agaagctagt ggctccaata tgttggttat
    23401 ctttgattct attctagcat ttgctgattt tatatccatt cttgttgaga atatacttct
    23461 atgaaccctc atatatttcc catctaaaaa ttcccctttt attgtatcta tatccttatt
    23521 tttttctagc tctttaaata tgttttcata tctacttaag aaaaattttc tctctggata
    23581 acattctttt aatttatata taacttcaaa tatattcttt tgaattggca tttggtcatg
    23641 tccatttgga agtattatat ggtcagtagt tgcaccctta tcaagaaccg gtaaatattt
    23701 atccattctt gttcttagct catcttcatt cattggtaga tattttccta ttgcataacc
    23761 taatggaagt atttgaacca aaacagaaga cccatcatca ctcttccaat taaactctgt
    23821 tttattagtt ccttttcttt cagaacatcc tctccaaaac attgagtatt ttatatcaaa
    23881 cccatttagt atttgcggca tttgagcact ttgaccaaat gaatctggta aatatcctat
    23941 tttcatatat tctccaaatt catcacaatc ttttattcca tacagtaaat ttctaagtat
    24001 ggattctcca ccaactacca tttcatcagt ttgagtatac caaggaccta ttattagcct
    24061 tccttcttga actaattttt ttattctctc cttacattca ggcttaactg ctaaataatc
    24121 ttctaatata gcagtttgac catccatcac ataatatgga tagttatgat tattttctaa
    24181 catatccatt atttcttcca tattatttac aagtaatatt ctagattcct cagttgagaa
    24241 gtaccattct ctatcccaat gcatatgagg aactatgtgt acattttttt tcattgctta
    24301 tcctccaaaa cttatctgat aatattttct gtctaattaa aaatccaata aaaatactac
    24361 tgagtttaca caatttttta atatttattt tatcactata attaaacctt tgatttaaat
    24421 atctaataac gtatctttta atgtatcttt ctttgatttt tttgtttcct agttattatt
    24481 aataatattg ctgatattat agctccaagt actgccgcaa agaaccatat tgatgctata
    24541 ataaatcctg acatacttcc ctctaaaagc gccaatgaga atatccccgc tccaggcact
    24601 tgaagaccta tattgaagaa tgctactata gacccagtta tggctgaacc agctactaat
    24661 gatggtataa catggattgg gtcctctatc ataaatggta ttgcaccttc agttattcca
    24721 gccaatccta atatccaagt ttgctttcct atttcctttt ctgattctga aaataaatct
    24781 ctgcctaaaa cagtagctat tgtaactcca aaagctgaga ccatcttaac tgatgcaaat
    24841 attgcataag gtataaaatt tccacttgct attgctccta ttgcaaatgt ataggctgct
    24901 ttatttattg gtccacctaa gtcaaatgaa accatagctc ctattattgc acctagtagt
    24961 attgcattta ttccagtcat cccatttaac caatttataa gaccattgtt aagtgctgct
    25021 acaggctttc ctattacaaa taacatcaat aaacctacta ccaaagaacc aactactgga
    25081 tatacccaaa aacttacgaa tccagctaat gtgcctttgg ctggtatatt cttctttaaa
    25141 aattttacta tataaccagc aagtaaacct cctaacatac ctccaagaaa tccactacct
    25201 attaaatcag ctgctagacc tgctgcaaat cctggtccaa gacctggttt ttcagctagt
    25261 gagtaagaca tatatgctga taatactgga accatcaatg tacctaacat ccctccagaa
    25321 agctttctta attgccataa ccaagaacct tcttgattgt atagttcttg taaaccaaat
    25381 gcttgagatg ctaatactgc aaatgcaagt atcatacctc ctgccacaat aagtggtatt
    25441 atatgagata ctcctgtaag tattgctgat tttacatctt ttttccatga tgatttttca
    25501 caatatgata tatcagatga tttttctata taatcacctt taccttgttt ttgtgctact
    25561 tctaaagctt cgtatattat ttcttttgcc ctttttagtg gagctgctac tgacgttttt
    25621 atgtttggca aatgttcaaa tctttctgta tttttaggtg ctacatctgt tgcatatatt
    25681 attgcatctg ccttttttat tatatctggt gtatgagggt ccaccatacc attagctcct
    25741 tgcttttcta catacacatc cacacctatt tctttgccag ctttaattaa tgcttctgct
    25801 gacatatatg tatgtgctat acctgcaggg catgctgtta ttgctaatac aatttttttt
    25861 gattcatttt gaatcctaat ctcttctttt atttgttctt tcttttcttt tatatctaag
    25921 ttattaaata actctttctt agtttttgaa tttaacaaca tattcatata atcttcatta
    25981 gacagtctag taactaactc agaaagtatt tgtatgtgtg tatttccacc ttcactttct
    26041 ggtactgcta ttaaaaatat tagctctact ttattatttg agtctacgct ttcccaattt
    26101 tctattggtg tctctaaggt catagctgca aatgcagctt ctttaactga cttagatttt
    26161 ccatgaggaa ttgcaagccc tctttcaaaa cctgttggag ataaagtttc tctgtacatt
    26221 acagcttcta aatattcctc ataagatgtt aacttgcctt catcatatag ctttcttgct
    26281 aagaacttta ttgcgtcttc ctttgttttt attttttgtt ttaaaaaaac aaggttttca
    26341 tttgtgattt tcttaaattc caaaattacg acctccttaa atcatttaat gttatgaata
    26401 tagcataaaa taaatcatga aaatgttttt ttgaaattat acacacaatt tcttcttaaa
    26461 aaataaggat ttgtgtattt atacacaaat ctctatctta accagtattg ttctattatt
    26521 gctctcataa tcatcataat tggaacctta tctgatatat tgtacccatt tacttgttta
    26581 attgttgaat agcaatataa acttatatct gcaatttgtt gcaattgatt ttcacatata
    26641 tgtgttagag ctactacctt acaaccctta ttaactgctg tctttgctgc ttctataact
    26701 tctttcgttt ctccacttaa actaagcgcg aaaaacatat cttgttcacc taattttcct
    26761 atatcatgaa gtacttcatg tctttggcta tagtaagtta tattatcatg cccacctcta
    26821 atttctttta ctaatatctc acataaagga atgttttgac ctattccata aactataata
    26881 ctcctagatt tttgaattat atctgctact ttatttattt tttcataatc tattagctct
    26941 aatgtctttt ttatatctga ttcaaaacca ttttttggct ctttatcctc attttttaac
    27001 tcttctttta aattgttttt tagttgagag aaaccatcat actccatttt tttacatagc
    27061 ctaactatgg tatttgggac agtaaaaaat ttatctgcta aagattgtat tgataatttt
    27121 actatttctt ctttgttttt tctcatatac tcaattatca tgtcttctgt atctgtaaat
    27181 ttatactcat atttttttgc tctttcataa aattccatag tttctcctcc atctatttta
    27241 ttattcataa gtaaaattca aaaatattag ctaaaatttt cgttaatata attataattt
    27301 aatattttta atttattaat gcatactact ctttttatta tacataaaaa atgtgtactt
    27361 cctatattga tagagatgat tcaggttttt attttgatac atctctatca atattttttt
    27421 agaaaataca catttttttc aaatacttat ttttaattaa aactttaggt aatctattct
    27481 ttatatataa ttattagact tgaaaatatc tttaaaatca accaattcaa tattatttct
    27541 ttttaaccac attttaactt ccttatcaca tagcattgca tgttcatata ctctctcttg
    27601 aataagagaa gatgatttta taagactata atctataaat cctggatgag taaccatcaa
    27661 tgttatatct ttatctaaat ttatatattt ttcttcaaat aatttaacaa aagatttatt
    27721 taacttgtaa aaatcattct gaggatattt acttggcatg ctaatacatg aatctaaata
    27781 atagggacaa cttggtatac cgtattcttt tgcagcatca cacattgctg atattgatac
    27841 atcttcttct ataccatgta tatcaatata ctcaggcaat ttactcatat acaacttgta
    27901 agtttcaatt tgagcacaaa cttcttcata tacttcttca taattaaaaa cctctttacc
    27961 tgtcagtagt tgcgttcttc ttattttgct agaaacaaat tttccacctt catctaacaa
    28021 attctttatc ttaatatgtt ctttacaaca aggttctcct attactaaat taacatgtaa
    28081 accaaaacat acatcatcat tttttaccat ttctatagct tgtcttgaaa aaggcatatt
    28141 caccatgatt ccagcacttg taacaatacc gttcttataa gaagatatta tccctagtgt
    28201 aacaccttct gaatatccta tatcatctgc cctaactact aatttcttca ttttatgcat
    28261 ctgcctgctt tctcaactca tcaagttgca tttccatctc atcctttact ttaacaacat
    28321 ccgttcctat gatgatttga actgatgtat tactcaattt aattacccct ttggctcctg
    28381 tttttttaat attatcatca ttcaaaatag tggcatcctt cacttctaaa cgtaacctcg
    28441 tcatacaatt attaagagtc actatatttt cataaccacc acaattttta attatctgct
    28501 tagccatata tgaaaaatta ccatgagaaa gttttaaatt cttttctttt tcactaactt
    28561 tatcacctat ctctatctca acttcatgtc ctggtgtttg tacattatct tttattatta
    28621 tatatttaaa tgttacataa tataatacaa agaatacaac tcctacaggt ataactaacc
    28681 acgctttatc tccataacta aaacttaaga catagtcaat tatacttgct ccattaaatg
    28741 aacccaagcg agcacccagt aaatatagta caactccacc tagaccagaa taaaaagaat
    28801 gaattatata taactttgga gatgcaaata aaaacgaaaa ttctattggt tcagttactg
    28861 ttgccataaa agaagtcaat gctccagcgg tcattgcacc ttttacttct tgcttctttt
    28921 tatcttgtgc tgttttatat atagcataag aaatagcagg tattgaaaac atcataatta
    28981 tgtaaaatcc tgtccagaag aatcctgcat ttgggtctcc tgccataaat ctaggaattt
    29041 ctcctcttat aacttctcct gttgatgttg tatattcacc catttcaaat aatatgtaag
    29101 catttaaaac ttgatgtaac ccagttggta ttagtgcacg gttacagaat aaaaacaaga
    29161 atattcctat tgcaccagct cctcccaacc aaaaaccaaa ttgattcatt gcagcttgtg
    29221 gatatatcca taataaacta aatatatatg ataaaattaa agcaaccact ggtgatagtg
    29281 taattacaca tttttctcct gcaaagaagc ttaaaaatgc tggaacttta actttatatg
    29341 ttttattgta agtccatgct atacaagctc caacaattat cccaccaaat acacctgcat
    29401 ttaatgtatc attcaataac tctaaatttg acttaaatat catatatgca actacggctg
    29461 caaccattac acttgtcttg tctttagact ttgaataagc actaactgct cctaataaaa
    29521 ataagtagtc cacatttgaa aatactatat tagatatatt agatatagct ggcacattaa
    29581 gcatatttgg accaaataat aatcctaata tacctgctgc tggcattaca gctattggca
    29641 ccagcatagt ctttcctaaa agtgacagtt gttgcatcat tttgtttaag aacctcttca
    29701 tatccaatcc cctttctatt tcggtttaat tttttgtgaa aacgatttat tcacatttta
    29761 tattttaaat attatatttg tttaaacaaa ttgtcaatat tttttattca ttttattttt
    29821 atcatttagt ttcatatcta aatcctttta agtatattgc tatatctagc ctatttttta
    29881 aatttatttt ttttattttt aaaatatgtt taaccatgta tttgaaacaa cccacaaaat
    29941 agttcaaatt tatccttttt atggtttata taattctatt ttcactatac atgtctaaac
    30001 acatttgata taatttgttc atatcagttc aataatatta ctaaagacct ttaaggaggg
    30061 ataggggtat atggctaaaa ataaattttc agagatatat gaagtattga aagaggaaat
    30121 acttgatggt aaatatacaa gtaatatgat gctgcctact gaacttcaac ttattgaaag
    30181 gttttcatgt agcagaaaca ctgtacgtag agcaatttct caattaaaca cagaaggata
    30241 tgttcaaagt ataaaaggca agggtgttgt agtgttagaa aatagttgtt ctaatgattt
    30301 ttttctaaat atgcacaatt tcaaaggtgt agaatcgatt gtagaagata aaaaggttaa
    30361 tactgctact agtgttctcc acttcagcaa aatcttaatt gataataagt tatctaaaaa
    30421 gacaggtttt aaagttggta gtgaagtata ttaccttcac agactaagat atattgataa
    30481 catacctaaa attctcgaca ttaactattt tctatgtagt atagttaaag accttgatgt
    30541 ttctatagca caaggctcta tttataaata tatagaagag tgtataggta caaaaattgt
    30601 ttcatccaga aagatattta aaattgaaaa agctacagag cttgagttaa aaacgctacc
    30661 tctaaatgat tataattgtg ttggtgttat taaaaatagt gtatatactg atgatggaaa
    30721 gctctttgaa tatacagaat caaagcatac acctgaaaca tttgtattca tggatgttac
    30781 tcaaagatat taaaaaaatt atgtttaatt aaacataatt tttttacgtt tagacacttt
    30841 atcccatact aacctcacta ttctatatac aattatatgt ttatacaaat aattatttat
    30901 tgtatatttt agctttaata ttagtttaat acttgacaat ttgtttaaac atattataat
    30961 taacttataa attacaggag gtatcaatta tgaaaaattg gtggaaaaaa gcaactgtct
    31021 atcaaatata tccaaaaagt ttcaaagata gcaacaatga tggtataggt gatataaatg
    31081 gtattataga aaaattagat tatctgtatt ctttaggtgt tgatttatta tggttgactc
    31141 ctatgtacgt ttctcctcaa agagataatg gatatgatat agaagattat tataatatag
    31201 acccaaaata tgggacaatg agtgactttg aaaaactctt aaaagaagct cataaaagag
    31261 acataaaaat aatgatggat atggtcctaa accatacatc tactgaacat aaatggttta
    31321 aagaatctaa aaaaagcaaa gacaatccat atcgtgatta ttatttctgg aaagatgcta
    31381 aacctgatgg ttctgttcct aataactgga tatccagatt tagtggaact gcttggaaat
    31441 atgatgaaac tactaaccaa tattatttac atttatttga agaaactcaa gctgatttaa
    31501 atttggaaaa tgaaaaggtt cgtgaagaat gttacaaagt actagaattt tgggctgata
    31561 aaggtattga tggttttcgt ttagatgtag tcaacctact ctccaaaaca cctggattac
    31621 ctgatgaccc tataacaggg ccaaaaggag atggaagaac tcattacgct gatggtcctc
    31681 gtatacacga atatttacat aatatgaatc aaaaagtttt caaacctaaa aatatagtta
    31741 ctgtaggtga aatgtcatct actacacctg aagagtgtat caactatact agagaaaatc
    31801 gagaagaact atctatggtt tttagcttcc atcatatgaa aacagattat aaaggaggcg
    31861 caaaatggac taatgaaatg ttctctctgg ataaattaaa aaaagcacaa tctgactttc
    31921 aatataaaat gtatgaagga aagggatgga atgctctatt ttactctaat cacgaccaac
    31981 ctagagcttt atctcgattt ggagatgata gattttttcg tcaagaaagt gctaagatgt
    32041 tagccataac actatttggt ctacaaggta ctacatacat atatcaaggt gaagaaatag
    32101 gtatgacaaa tgcctacttt actaaaatag atgaatatga tgatgctgaa agtaagaatg
    32161 catactatca tatgattaaa tctggtattg atgaaaagga ggcattgctt atacttcaac
    32221 aaaaatccag agataatgcc cgtactccta tgcaatggga taatactatt tataaggggt
    32281 tttctaatca taaaccgtgg ttaaaagtta ataatgacaa tattagtgta gaaaatgagc
    32341 ttaatgattc tcgttcagta ttttatactt atcaaagatt aatcaaatat cgtaaacaat
    32401 atgatatttt tacagaaggt acttatagat tgttagatga aaatcataaa aatctatttg
    32461 tttatgaaag agagtataaa aaccaaaatc ttcttgttat aagtaatttt actcatgata
    32521 atgtagtata taaattacct gaaacttatt tgaataaatt aaactacaca atacttgaaa
    32581 ccaattacac tagagatata attgaaaata aaatggaaat aaaaccttac gaaagtataa
    32641 tgatatatta taaataaagt tatttatata aaaattggta taacaccagg gtgttatacc
    32701 aattttacta aattcaaaat aacatttata gaatatctat agattacaat aattatttga
    32761 cttctacatc attatatttc tttgaatata ctttagtcat atagaaatat ataggaacta
    32821 caattatcat cccacctaat ccccaaacta tcttcatagg ttcatcaata cctgctttta
    32881 tcagtaataa tatacttata ataactgcaa atattggtat aattgctcct ccaggtattc
    32941 taaaatttac atctttatca gcatacttct ttctcaatat tagtacagct aaacatgtag
    33001 gaatatattg aataaatcta gatacaacac ttatttgaga taatgttgtg aatgaacccg
    33061 accatgctat tagtaaagtt cctatgacag atactataat tgctatataa ggagctccat
    33121 ttttattagt ttttcttata gacttaggca ttaatccttg ttcaaccaaa gcagcagcag
    33181 accttggtgt aaatatggat gatgcaatat ttatacctcc aacagatact atagttccta
    33241 ttgtaattat atacccacct ataggaccta gcatcttaat ggcagcatct gcaacaggag
    33301 tagctgaccc tgccaagcca ttacccaaca cccctatact tattcccagt ataagcatat
    33361 atattactga tactacaaac attaccatta ccaatgcttt aggtacattt ttcttagggt
    33421 tctccatatc ttttgctgca acagctaaag attcaaatcc tgtaaaagca taaaataaag
    33481 ttatagatac tgctacaaaa gctgatgacg cactttgacc cttttgtatt attacaaatg
    33541 gggtaaaatt agaacccttt ataaagaata atcctacagc tataaataaa actattggta
    33601 ataattttcc tatagtcatg atattattta agtgcttaga ttgtttcatt cccataaaat
    33661 ttataatccc aagtcctaca actaatatac caattattat atttttaata agtggttctg
    33721 ctgcttgtgg ccatacagat gatagtgtta cagcaaaaaa atttgccata gtagcccaag
    33781 caatcatact aactatccac ttcataaatc caacttcaaa tccaacaaaa tctccaaatg
    33841 cctcttttgc ataaagataa ggccctccat cagtatcaaa ccaactacct gcttcagcaa
    33901 aacataaagc aatagaaaca gctaaaagca tacaaaaaac taatactaat atacttgctg
    33961 gacctatatc tgtataagca tcaccaggta acccgaatat acctgaccct attattgcat
    34021 tgattcctaa tagtacaata ctccaaaaac ctaacttaat gcctttttcc attgttattc
    34081 cccccaaagc caacttaata tatttatatt ttttatttag atgcttcttt aactattgcc
    34141 ataaatattt ttttcatatt atcataatct ctagtcatca tttctggatg ccattgtatc
    34201 cctattacaa actcacttcc ttccttttct atagcttcaa ttaaaccgtc tttagatgta
    34261 gctgaaacaa tatatcctgg tgctactttg ttgacaacta agtgatgaaa actattaact
    34321 aaaacttctt tttctcctaa tatttcatat aattttgtcc cctcctttat ctgtactgta
    34381 tgtgtaggta cattagatag atgttgctga ttgtgtttaa tataagcacc ttcaattaaa
    34441 gataaatctt gatacaaact acctccttca gttacattta taatttgttc tcctctacat
    34501 atacctaaaa ctggtttttt catatctaaa gcatgtttta ataatttcat atcaaaacta
    34561 tctctctttg gtgatattgc ccccaactta ttatgaggtt cttctttcca tatgagtgga
    34621 tttacatctt gtcccccaga tagtataagc gcatctatat tagatacctg ctcctttata
    34681 atctcatcat catatacaat cggtacaata tatggtatag ctttacacat aactactgat
    34741 tgtatataat cattatttac ataagccctt tcatagccag gaaacattcc tccttcatca
    34801 attagtaaat tacctgatat tcctattatt ggatatttct ttttaaccaa tttcttcacc
    34861 ccttaactta tttgtgaaca ataaaaaatg ctacttctct taaagagcaa gtagcatacc
    34921 aagattttat ttatttgaac attataattc attttagaaa tacttttttt gaatacaagt
    34981 aatataaata actttattta cttatatttt attttaatcc tgtttagttt gactttttta
    35041 aattgtttta agtacaacta ataggtaaaa taggcattta gattttcctt tttatcaacc
    35101 tattaaatac tgtttaatat attttttccc cttctattta attcacttcc ctttctacca
    35161 gattcacata ttatcaaatc cagctctttt aacatattaa gtattcccct tacctcacct
    35221 tctgttatac tgtagcctaa ttcatttatt gaattaacaa taccatttct acctatattt
    35281 ttattaagat tattggcatt ttttaaacac tccattacta actttacttt ctctatagat
    35341 gttctatcct ttaatatgct tatttcttta ctaaaatctt cagttatatt taataagtta
    35401 tgaggtaaat catttattaa aataacatcc tcagaactca taatgttaat atacatagca
    35461 gtatttttta attctcttat attacctggc cattcatatg cattaataat gtcttgaaca
    35521 ccttgactaa ttgctctctt ccttttcata aaatgcttta acataacttc tatatcttct
    35581 tttcgctctc ttaaaggtgg tatatttatg ggcaatacat ttatcctata gtataaatct
    35641 gctctaaatc tatctccttt tatcatttct aataaatttt tatttgtagc cgcaattact
    35701 ctaacatcta tatctattac attttctcct ccaaccctca ttacttgatt ctcttgtaat
    35761 actctaagta atttgctttg taagtatata ggcatgtctc ctatttcatc caaaaatata
    35821 gttccattgt tagcctgttc aaataatcct tttttacctc cttttaaagc tcctgtaaaa
    35881 gcaccttctt catatccaaa aagttcactt tcaagtaaat tttctggcat agctgcacag
    35941 tttatcgcta taaatgactg actatgtctt ggtgatgcat tatgaataga ctgagccata
    36001 agttcttttc ctgttccact ttctcccgtt ataagaattg tataatcaga ttcagctatt
    36061 ttcataccta atttcttagt tctttccatc ttcgttgaaa ctgtttttat atcatcaaaa
    36121 gtatatctag caacttgtcc cttttctcta agcttctttg ataggttttg ctccaatttt
    36181 cttatataag ttatttcttg aatacaaaaa taatacccag taacccttcc aaaactctct
    36241 atattatact tatttacgtt taagtactta ttattaaatt ttataacttc atcatgtgcc
    36301 ttactcaaac taaaaatctt tttaatattc tcattctcta attcatctat atttttccct
    36361 ataatattac tttgaatatc tagtatatcc cttaaactat tattgcaaat aattatgttt
    36421 cccttatctg ataccattgc catacctaag tttgataaat ttattataga atttaattca
    36481 tctgctttta tagtaagttc tttgtattta gtatttattc ctgaatataa actaattatt
    36541 tcatctgtat acttaattag cctctcagta atttctttat catccagttt gagttttgct
    36601 attatttgaa taaacgttga actatcaata tacctatggc ctaaatcaat aacttcttct
    36661 atatattttg gaactaaact tgactctcct ggcgttatag ctatcttaat atttctatat
    36721 tcttcattgg gattgtatgg tataaaattc aaatggttta ttcctatatt gtataaatta
    36781 gatattgttt ccaatacagt atgcttatta tcgtttacta ctaatacatc tatttcttct
    36841 ggcaatgaaa ataacttata tatatccttt tgctttatgc tcctatttat agttattatc
    36901 tttgagacat cttctacata tttttttatt tttaatactc tatcatctat cattaccaat
    36961 attatgtcat cttttattaa ttccttctct gttattttat ttaaataata attttttatc
    37021 attactttat ctttaaatat catttctaga tttttattta agaaacattc taatttagac
    37081 aacccatcag ttactactgc tattgttttc atttgattac tccttttaaa atatactcta
    37141 ctcttttata atatacatct gattttcact ttaatagcac aaaaaattgt acatttagta
    37201 tactaacata ttcctttatt agttttatgt ccctaaatat catagttgca atatctatca
    37261 tatttcttaa acttctataa agcaaaaatt gtgccaaatt aataattttc ttaattttat
    37321 tctaaagttc ctcaatagta tttacagact tagcacttat agcttataag tctttatatt
    37381 attaatctat ttttataggt taattgtaca aatcatatat cacttttacc tattaaatgc
    37441 attataaaaa agatggaata acgattcgtc actccacctt ttttgtaata aaaaattgta
    37501 tatataaaca ctatttacta ctcttctaag tacaaacagt atctacatac tataaccttt
    37561 caccattaga ttctattact tgtttatacc agtcaaaact ctttttctta atcctagaca
    37621 agtctccact tccatcatca tacttatcca cgtatataaa cccatatctc ttggtcatct
    37681 caccagtaga tgcacttact aaatctatac atccccatgg agtatatcca aataaatcaa
    37741 caccatcttc tacagcttcc ttcatttgct ctatatgtgc ttttaaatac tctatacgat
    37801 agtcatcatt tatcactcca ttagattctt ttttatcata agctcctaaa ccattttcaa
    37861 ctaccatcat aggtattcta tatctatcat aaatttcatt tagtgcatat ctaagccctt
    37921 cagggtctat ttgccatccc cattcactag acttaagata tggatttttc actccaccta
    37981 atatgttgcc acttgtatta tcttctttag aatcactact tacacatgta gacatataat
    38041 aactaaatgt ataaaaatct acacaacctt cttttaaaat ttctaaatct cctttttcca
    38101 tttttatttc aatattattt tctccaaaat atctattcat ataagttgga tattcgccac
    38161 gcacttgtac atctccacag aaataattca ttactctact atctctttga gttttaactg
    38221 catcatctgg attacaagta taaggataca ttgttgcaaa tatttgcata catccaactt
    38281 gataatttga atctatttca tgagctaatt taacagcctt agcacttgct acaaattgat
    38341 gatgtaaccc ttgaaaacga agttgaggta tatctacttg gtttataaaa tctgtagtcc
    38401 cttcatttaa tattccttgt gataagtagc ctcccatagg cattgtagct gaatttattt
    38461 cattaaatgt cagccaatat tttacttttc ctttatatct tttaaatatt gtttcacagt
    38521 aattcatata caagtctatt acctctcttg aaacccatcc attgtatttc tctgttattg
    38581 caaatggtat ttcatagtga gatattgtta ttaatggttc tatattatat tttaaacatt
    38641 catctatcac attttcataa aatttaagtc ctgcttcatt tggttctttc tccattccag
    38701 ttggaaatat tctagtccat gctattgaaa atctaaatac cttaaatccc atttctgcaa
    38761 ataaagctat atcttctttg tatctgtgat aaaaatctat tgcttcatga cttggataga
    38821 atgttccttt ttctataact ctcgttattc ttttacttac tttttgagaa cccccagtac
    38881 acatatctga acaagaagga cctttcccat ctaaattcca tgctccttca cattgatttg
    38941 ctgctgttgc tccaccccat aaaaaatctt ttctaagtac cattactcta cctccaataa
    39001 tttatttata gtaaatttat tttaaaataa aaatacatat atctaatttt taattaataa
    39061 aactctcttt taagctaaaa cagttatcaa ttcctgacta ttatttacat gcttttgtgt
    39121 attttctata atatccacat aatcagaata attagtaaca actactggtg tttctaagca
    39181 atatccttct tttgatatgc tttctatatc aaaagttatt aatgtctgac cttttttaat
    39241 cttatcccct tgattaacaa aagatttgaa atgtttacct tccaactgaa tagtatttaa
    39301 ccctatatgt atcaatagtt cacaaccatt atcagatata attcctattg catgttttgt
    39361 tggaaataaa gtcattatag tcccatcaaa tggtgccact acttttcctt ctttaggtgt
    39421 tattgctaca ccttttccca atatacctgt tgaaaacgct gcatcttgta tctgctccaa
    39481 agcctttact tcacctttta ttgggctata tactacttct cttttgtttt tttctacaac
    39541 gccttgccca ttattatctt tagaaatttc tactatagaa tcatctttcc aaaaaaataa
    39601 tgttaaaaca aatcctacta caatagctac tattattgct attatagttg cataaacacc
    39661 tttcatatct ccagttgttg gatttatata attaggtata ccaaatattc caagtccacc
    39721 cattacataa ctttttacat tcattatccc cattacagct cctgccacag caccacctac
    39781 acaactatat atgaatggtg tttttcttgg taaagttatt ccatatattg caggctctat
    39841 tacaccacat atccctgaaa taactgcagg tatacacagc tcttttatct tcttatcttt
    39901 taatttaaag tacattgctg ctactactga tgtttgtgca aaagatggta caaatgctcc
    39961 cactaatatt gtgtcaaacc ccatagtcat aaggtttgac atagctattg gtactaatcc
    40021 ccaatgtaga ccaaatatta caagcacttg ccacaataaa ccagttaata caccacataa
    40081 aattggtgaa aagttaaata aagctaaaaa tccatttcca actatattag aagcataggt
    40141 agctataggc cctataacca taaatcccaa tggtaaagaa actaataaaa ctgccattgg
    40201 tacaaagaaa ttctttacta catctggtat tattttagca aacactttct ccaacttagc
    40261 tgctaataat actattataa ttactggtac tactgttgaa gtgtagttca ttgctattac
    40321 tggtaatcct aagaaagtta tatatactgg tgattcaaat attgttccag taaatagatt
    40381 gtataaaggt tgacctatag atgctagagt atctaattga atacctgaat agcataaaac
    40441 tgcacctata agcattccaa taaatggttt taatttgaat ttcttagcag ctgtatatcc
    40501 aagaattatt ggcatatact ggaacattgc atctccgatt gcatttaaca taatatatgc
    40561 accatcagtt tctccataca aacccaatgc tgtaaatact gcattaaatc cttttatcat
    40621 accagcagca cacagaactc ctaatattgg ttgaaaaact cctgatatta tgtctataaa
    40681 cttattaagt aatccttttg gcgcatctga atctgaatta tctattgtat ctcctcctaa
    40741 tccagccaaa tcgcacacaa cttcataaac atctggaaca tgattaccaa taactacttg
    40801 gtattgacct gcacttttca ttacagtaac tataccatcc atttttttta gcacatcatc
    40861 atttgctatt gattcatctt ttaatttaaa acgtagcctc gttatacagt gagataaact
    40921 gctaatatta tccttccctc ctacattctc aatgatttct tttgatagtt gttcatatct
    40981 tctcattttt tctcctccat tttctttttg aaatatgaag gtttggccaa cttaatgtca
    41041 ccacccaata tatataaacc taattattag gatttatatc tgacttaata cttttactta
    41101 ccactctttc aacgtgaata gttaagtata atttttcctc atctgttaaa tcgtagttgt
    41161 attttttatg tataaattca tccaactttt ctatgcattt ataagcattt ttatatttaa
    41221 cttttattac atttagtaaa tcatcttcat tattttcatg taaattatta ctaacaattc
    41281 gttctgcaaa atactttaag tgagtaataa atctatgata ataaactgaa tcttcatcaa
    41341 atactattct gtaatggtat ttaaccatat ttgaaatttc ttgtattatt tgtgtaatct
    41401 catatatagt ttttatactt tgatccattt gtgcattaac tatatgcagt gctataaatc
    41461 cagcttcatc ttctgatagt ctaatttcaa attttttttc tatataatcc aatgcttcta
    41521 accctatttt aaattcactc ttataaaatc tttttatatc ccaaagtaat acatttttta
    41581 cacttactcc ttcacgagct ctttctattg cagtatacat atggtctgtt aaagaaatat
    41641 aaatactatc atttaatctt ttccctagtt tttctttagc atataaaatt atttcatctg
    41701 ataattccat atatcttata ggaatatctg ctattagttc tttaaattta tcagatatat
    41761 ttgggtctga aagtttgaat atcttatcaa ttttatcctt tgatatatgc tctcctgtac
    41821 gttttttata agctaaacct ctccccataa ctactatttc cttatgattt tcatcagttg
    41881 taataattac attattattg agtatttttt caataatcat tgtgatgccc cctttattag
    41941 cattaagtta aaataaatct tttttaatta tttagaatat ttaaaatgta atttattaca
    42001 taaaaaaaaa cctatttatt acacattaaa tagactccca tgagtcctga gtaataaata
    42061 ggtttagctt gtatttaaac aatcactatc cgtttgtatt ttattttaat tattatttat
    42121 tatatgttag caaatttatt ttttagtgtc aactttttct caatatttta attctaaata
    42181 aattatataa attgcatctg caaagtacta tttttataaa taaacaaaat aaaaaaggta
    42241 ttacttgctt taagtaatac ccatttattt ttaatttaag tggtttttct taatctcatc
    42301 aagtagtcca tcttctaaat aaatttctaa tgcacatcct accattgcct ttactgcttt
    42361 gtgaagttta tcataagctt cctcagaatt tgcataattt agaaattctt tttcatgaac
    42421 atataccatt ggacttatat ctaaggctat ttctgtatac atagtagggc aaacttgact
    42481 tacattacct atatctgtag aacctactgg accatcttta ccttctaata tatctgttat
    42541 acctgactca atcaaattat tctttgttat cttttgaagt actttgttat ttattatgtt
    42601 gtcaaaagaa ttttcaaagt atctataaga aagcttagct cctgtcatta actctgcacc
    42661 ttttgcacag tttataactt ttttagtcac ttcatctaaa taacttcttt cagcagctct
    42721 tacaaaaaac ttacaagctg ctttttctgg aactatattt ggagcctctc ctccatttga
    42781 aactattcca tgtattctaa catctgattt tacatgttgt cttagtgcat ttatcccagc
    42841 aaaagtaagt tgtactgcat ctagtgcatt aactcccata tgagggtgac tagctgcatg
    42901 tgctgatacc ccttcaaaac taaactctaa tgaatctatt gccaaagcct tacagtttat
    42961 attattaaaa gcttctatat gcatttgata acttacatca acatcatcaa aagctcctgc
    43021 cttaactaaa tcacattttc ctcctgttgt ttcttcagct ggtgttccta ttagtacaat
    43081 ttttccttta aaattttctt ttaacttact taatactata caagctccta gtgtagatgc
    43141 tgcaatccag ttatgcccac aagcatgagc tggttcttta ttgtctccat atccaggaag
    43201 tgcatcatac tcagctaaaa atgctatggt aggtccatca ttatctccta attcagctct
    43261 aaaagcagtc tccatattac aatatggata aactgtatca aatccatatt ccttcatttt
    43321 ctctactaag tattttgatg atatgtattc ttcttcccct aattctggat tgttaaatat
    43381 aaaattacaa acttcttcaa tttcttttcc aagttcattc tctatctcat aagctctctt
    43441 ctttatactc tctatcataa cttacctccc actagaatcc tagatataat cctattgtca
    43501 aaaataccat tacaagaatt gttaatacta ccattaaagg aattacgaat ctaacccact
    43561 ttgaccaagg aatattggct actgctagtg ctcccattaa aacacctgat gtaggtgtta
    43621 ttaagttagt tacacctata ccaaattgat aagctgtaac tatcatttgt cttgatacat
    43681 ctactaagtc gccaagtggt gcaagtacag gtattgtaag tgatgcaagc cctgatgctg
    43741 atggtattaa aaatgctatt aatccttcta ttaagaaagt taaatttata aatatagttt
    43801 ttggtaatcc atttaataaa tttgctgcag aatttaaaat tgtatctatt ataaatccat
    43861 tttctgctat tattacgatt ccacgtgcaa atgctataca taaccctgct gtaattaaat
    43921 ctcctgctcc acttataaat gaattaacta tctcatcttg tttaagccct gatgctattc
    43981 ctgatattat acctatagct gtaaatatca ttgcaatttc agttatgtac cactctagtc
    44041 ttaaaactcc atatatcata atcgccatac ctattgtgaa aatggctatt accatggctt
    44101 ctctttttgt aaattccttt atatttgtat cactattaac cattgattct tgatttagtg
    44161 atatatcgtg tactaaagat tttttagggt cttttttaac tttattggca tacatcataa
    44221 caaatcctat agatatagcc atatatatta taaacataac aactctaaac tctattccag
    44281 aacctggtac tatttgtgat aatgcttgtg ctattcctac agagaaagga ttcgtagtag
    44341 ctgctccaaa tccagctgtt gctcctataa atactgtggc cactgctgtc aatgaatcat
    44401 atcccatact ggtcataagt ggaataaata tcatataaaa aggtagtgtc tcttcactca
    44461 ttccaaacgt cgtcccacct agtccaaata gaatcattga aatcggtatt attaaaaatt
    44521 gaaacttctt cattttattt acagtatgag ctataacagc ttctatagcc cctgttttat
    44581 taactatccc aaaagctcct ccaactatta aaacaaatcc aactacttca gctgcatcta
    44641 taaatccgtt tataggtgct gtaaatacat cagtaattcc ttgtggattt gatggtgctt
    44701 tttcataagt tccagctaca acaactgacc ttccgtctat atctactctt tcaaaatttc
    44761 cacttggcac tatccaagta agaattgcca ttaacactat tagcgaaaat attattgtaa
    44821 gtgtctgtgg catagtaaac tttttctttt ttaaactgct catttttaat ctccccactt
    44881 tttaaattaa atattttatt attgcatccc tttatcctat tttgtataga tgtaaaaaag
    44941 ctcaacatgg atacatgttg agctaaatgt gctaaataca acatacctgc atcttccctt
    45001 tatcttgttt aagatagctc tccatcaaaa ttcgggataa ttttgatgac agtactgtac
    45061 ctattaaata cagtcccagc actagctttg gacactagca cttcggcact tactcctttt
    45121 atattgcctc attggtttcc aacctcttca atattaatct tagtaaatgc aacctctacc
    45181 tcaatctgtt cgttagatga ggtaattctt attaattttt attgattcaa agtaattgta
    45241 gcataatatg tcgagattgt aaatattttc agtataataa ttcttacttt atgatgtcat
    45301 tccatttttc acaaataatt ttttctccat ataatttaaa tactgtagta tattttaaat
    45361 acacacaaac tttccacaaa tttttaatta ttataatacc tttataataa aaaataaaag
    45421 ccatgtaaac ttatcaattt acacagcttt atatacatta atctactcca agtacagctt
    45481 taagctttga tgctattgta ataactcgtg gaccaaatat aacttgaact gagtttttct
    45541 ttttaacaac tccacttgca cccaattcat ttacccagaa accatcttca tagaatccat
    45601 caattgaatt tagctctacc ctaagtctag ttgcacagtt tgtaacattt tttatattat
    45661 tacttcctcc taaaccttct attatagaat gtacaagctt tgtatcttta tcatctactt
    45721 cctcttctac tgcattgttg tatgctgttt taccagcatc tacagattgt ctttctctat
    45781 attctttctt actataaagc ttaacttctt ctgaacttct tcctggtgtt tttatatcaa
    45841 acttcttaat tgcccatgta aatattatat aagtaactat aaaaaatatt ggtatcaaaa
    45901 ataataatgg taacgcatcc actttttgtg gttgtaataa attaggaaac atatttctaa
    45961 atgattctcc cattatagaa acattaaaca tttctgtaaa tacatacgtc aatcctgcca
    46021 gtggtacata tgctacaaaa tacaataatg gtgcaacaaa taggaatgta tactctattg
    46081 gttccgttac ccctaccaac atggctgtta ctattgctgg tatcaatata cttattgctt
    46141 ttttcttatt agcattatca gccaacttat acatagctaa agctgctgct ggtaatattg
    46201 caaaatgtat taataaacga cctgttgtga agttacgagt tatataagat agtgtatctg
    46261 ggtctcccat ttgagctaat cttatattat taacaccctc gtaaattgct ccacctactt
    46321 ccattgttcc acctactctt gtatattcta tagggaatgc aattaagtga tgtataccaa
    46381 atggtaataa tgctttatcc attgctccaa aagtaaatgt tcctaataat ccactagaac
    46441 ctatccaatt accaagttta gataatgcca accctactgg tggccatata aagtacattg
    46501 ctgtacccaa tactattgat gctataaaca tcataatcat tgaaaatctt ggacctgaga
    46561 aaaaagataa tgcactagga agttctatgt caacaaattt gttatgtact gcactagcta
    46621 caaatccagc tattatagct ccaaatatac tacaatcata tgaaaagatt cctaagaaag
    46681 aaccatataa agaacttcga ctatatgctt ctgcctctcc taatccagat gccataaacg
    46741 cttctgctgt aactgtatct gaatttatac catttgatgc taacaatgta cttataactg
    46801 tgtgcattcc tataaataaa acaattcctc ctaatgcacc ccatcctttt tcctttttag
    46861 ataatccaaa agctatacct gtaacaaaga atattggtaa gtatctcatt accataaatc
    46921 ctaaatcgtt tattacttta aagaatacat ttataaatga cccttctttt attagtccgc
    46981 taagccctaa tgcagaaacg ttttccatat ttgcaaaagc tgaccctaat cctataaata
    47041 ctcctgctac aacaagtaat ataacaggta tcatcattgc tccagaaagt acttgtaatt
    47101 tatccttcat agtattattt cctccatttt tattgaaatg ccaggctata taatgcatat
    47161 atatatactt ctattgcttt gtataatgta tctatactta tattttcatt tgcctgatgt
    47221 gaatttggat tatctccctc tattgtgggt ccaaatgcaa ctccattttt taacactctt
    47281 gcataagatg cagctccttt tgtaaaaagt tcggcttctt ttccaaaacc ttgtttatat
    47341 gcacttgata atttatttat taattcacta tctggtttta catacagtaa ttttaaatcc
    47401 ttatatatat caactattaa attttctttt ttcccaattt cattaattct attagttata
    47461 aactcccaac ttataccctt aggatatcta aagtctattt tcatattcaa ttcatttttc
    47521 tctaagaaga tactcattat acatattgta gaaactccca tatctacatc ttctttatat
    47581 atccctaatt tttttccatg attatcatct gtaaagtatg aatttaaaat atcccttatt
    47641 ataacgcctt tatcatttag ctttttaatt ttctctaaat cttttgctaa actaaatgca
    47701 cagttatatg acctgtgtgg atttctcgat aaagcccttt ctcctgtata tcttactaat
    47761 actttacctt cttctagttc ttctatctct gtataataag ctaatgattc tactaaatct
    47821 ttcttatcat cagttttaaa aacagcctca attttgtcac atacaaatcc atcttctttg
    47881 tttgacttta tatcaactaa attgtgattt ctaaatttat ctttatctat tttctttctt
    47941 agattgaagt ataaaatacc attttctcca tttactattg ggaagtttcc atctggagaa
    48001 aatgcaaatt ttggctgttc attgacacta aagtaatgct ccatacattc ccaagtcgtc
    48061 tcttcagctc cacccactat caatctaatc tttctcttcg gcttttcatt aagttctctt
    48121 aagaaaagta atgcatataa aatccctatc aaaggtcctt tgttatcatt aactcctcta
    48181 ccataaagat agttatcttt ttgacatact ttaaaaggtt tactcttcca aagttcttta
    48241 ttatatattg gcaccacatc tatatgatta agcaccccta ctacttcttc tccttctcca
    48301 tattctatat gaattgcata tccatcaaag tcttttacaa caaaatcttt gtcttttgct
    48361 atttgaataa gcttatcgaa ggcatttctt atttctatcc caaatggttg atttatatct
    48421 gttgtacttt catctctaat tgaaggtatt tcaactaaac ttctaatatc ctttatgata
    48481 ttttcttgat tggattttaa ataatcaata attagttctt tgtttactat catcttaatt
    48541 atttatcacc cctttatatt aattttacaa agcatttcag ttcatatcta cattataaat
    48601 tttgaatatt attttttcaa tatttcaaat acttctctaa attaccataa tgatacggta
    48661 aacaatcaac agattatgac aaaatcatac tatacctatt actaatattt taacttatct
    48721 attacgtctt ttttaatgta attttattcc aaaaatagat ataaaaagat gtagccttag
    48781 ctacatcttt ttatatctat tcaacttcca ttattatgct attaatatct acatttccag
    48841 gttctatcct atcttttact gttctcttaa cagaatcagc atcatccatt ataactacag
    48901 gagttattat atttaaatca tttgacgtta taaaatctaa atctacttcc attattaaat
    48961 ctttagcttt taccttatct cctgccttta ccttaacatc aaacggcttt ccttctagtg
    49021 acattgtctc taaacctaca tgtattaata tatttgtatt atcattactc ttaatcatta
    49081 ttgcatgctt tgtatcaaat acattgacta tttcaccatc aacaggagaa tataacttac
    49141 cttcatttgg tattattgct actccatctc ccatcatctt ttgggcaaaa actgcatctg
    49201 gaacatcttc taagttcact aaatttccct taaaagggct atatattttc tcaatcttat
    49261 tttctttttt cttaaatata ttaaacataa cacactctcc atattataaa attttatgat
    49321 aatatttttc ttaaagcttt tgctacacca tcatttacat tagtatcagt tacaaaatct
    49381 gccatagatt ttacttcatc tgtagcattt cccatagcaa taccaagacc tgcaaattta
    49441 atcatggata tatcattttc attatcacct atacaaataa cttctttgct atctatacct
    49501 aatatatcac aaaatttttt aactgcaata ccttttgaag tacctttact cataacttca
    49561 aaatttatct ttccagaact tactacttct aaatcttctt gttttttaaa ttcttccttt
    49621 gcttctctca aagaatctac gttttctgag aaaagtatac attttaaaat ttcattatca
    49681 gctttttcta ataaagtttc acaacttgca ttttctatta tttcaattct gtcttcttct
    49741 ttatttttac tatttattaa tctatatgga tgttcttcac ctatttcatt gttggatata
    49801 actattttac agcctttaaa atgtttgttt aaattatact tttcacctat tgtatagatt
    49861 ttctttatag cttctttgct caaaaccttc ttatactcat atcccattaa tttaaaatat
    49921 gtcccattag ttgatataat atatatatca tcccctaaaa cacttgtaaa atatttcata
    49981 gcattataag gtcttcccgt aactaaggct accttgatac ccttttccat agcttctttt
    50041 agtgctttaa tattttcttc tgatacttca aatcctttcc ccatcaaagt accatccata
    50101 tcagtacaaa ttaatttata tcgcatttgt atcacatcct agtaagattt ttctaatatt
    50161 tcaacaaggt cttctttctt tccaaaaggt aaaaatccta aaactggaca gcttgttatt
    50221 tgttctaatt gttcctttgt tacattaaac tcagacattt ttgtcttaag tcccactctc
    50281 tttaaaaaac tttctatttc atttcctaaa gttttagctg cttcatcatc ttccactttt
    50341 tctaattctt ggtttagcat tctcgctact ttggcaaatt tctctatatt atccttatag
    50401 aacttttctg caaattttgg gaacactata gccaaagcct caccatgagg aatatgtgta
    50461 actcctccta ttatttcact tagtggatgt ggtgctgctg ctccagcatt tgccagtgaa
    50521 cttcctccta atgtatctgc tactgctaac tttgttctgt attctatatt atttttatct
    50581 tccattactt taggtagata gttaatgact aattccatag atttttcaga cattatctct
    50641 gtaaagtcat tagcatttgg atttatataa ctttcaaatg catgagtaaa agcatcaaat
    50701 gctgttgatg cagtaattct ctctggcaga gttaacataa gctctgggtc aactatacat
    50761 tcttttgaga aacaattttg atgaaatatt gtatttttct cgcttcctct cgatattact
    50821 gctgcttgtg taacttggct tcctgttcca gaagtagttg atacagctat taatggtaat
    50881 tctttttcac ttaccttatc gtatatttta aatggatttg tatatgtact aaacatataa
    50941 tcccaatcta ttttttctaa accaaaagtc aatgctaata cttttgcagt atctatactt
    51001 gaacctcctc caacagctaa aacaacatct acagattctt ttttagctaa ttcgaaccct
    51061 tcatttatta attctacagt tgggtttggc tctactctat caaagtgtac aacttctata
    51121 cctttttcta ttaaattcaa tgtaactctt ttatataact tgtctaatgg ctctacattt
    51181 ggagtagtaa ctaacaaaca tttctttcca tattttccta ttattctagg tagttcactt
    51241 aaagttcctt gtccaaaatt tattcttgat ggttgataaa agttaatcat atttttcctc
    51301 ccttaaatta ttgacttcat attcaaatta tacatatcat aggattttta aggcaatacc
    51361 ttcactgtta actcttattt actaaagctt atggcaatta ttagacaata aatgttttat
    51421 tattaccata tcattatgta aattttatta agtacccatg ttataatttt ataatcaatc
    51481 ttattgtata attgacttaa taataaaaat agagtatata cactttttta tgacaaatag
    51541 cttaaaggag aattaaatta tgaaatattt gatagacagt gctaatcttg atgaaattca
    51601 aaagtgttta aagtatggat ttaagggaat aacagctaat ccatctatgt atcttaaaga
    51661 gaaggtaaac ttccatgaat ttattgagac atgtactaat ttaaatccag aaatattaac
    51721 tgctgaagtc attggaaata atttatacga attaattaat tcatgtgata agatattaaa
    51781 tataaataaa aacactataa taaaaataaa tttctctgaa gttggcttag aacttataaa
    51841 catattaaat agtaaaggtg taaaaactgc atgtaccttg attttcaatg taaatcaagc
    51901 atcattagct ataaatgcag gtgctgatta cctattccct tttataggta gaaatgatga
    51961 aaatgggaat gatggtataa aaaaccttac agaaatatgt aaccttatat caataaatgg
    52021 atataatact aaagttgttg cagcttctat taaaaatgta ttccacttaa ctcaagcatc
    52081 tctttgtgga tgtgattatg cagctgtaac atatgattta tttaaaaaag cttcttttaa
    52141 ccagcttaca gtagatggtg ccaatacttt tgaaagagat tggtccaagg ttaataaata
    52201 gtaactaaga tttaggaggg tttgcattga acaaaagatt attagatatt gctgaattat
    52261 tactcaacag ctcagactat attactgtag atactattgc cgaagaatta aaagttagta
    52321 ataaaactat tcgtaatgac cttgttattt tagatgaatg gcttttagag tttaatttat
    52381 ccctagataa aaaaactggt tcaggggtta ttattctagg taatgaagat ataaaactta
    52441 aagttattag agatatcaat gataaatcaa attgtatata tgcatactct cctgaagata
    52501 gaaaaaggta tattctaagt aaattattta ttaaaaattc taagtttagg attcgtgata
    52561 tttgcaatga actacatgtt agtagagcta ctgttcataa agacttggta gttattcaaa
    52621 attttttgca aaactttaaa gtgactctag ttagaaaaac caataatgga gtatatttag
    52681 agggaaaaga gaaagatatt cgaaaagcag tttttgaact tgctactgtt aataaaagtt
    52741 atactgaact taaagaaata ttattctcga actctaatga atgcaaagat accccatcaa
    52801 caaaaatatt taaggagcta tttaactatg attttgagaa attatctcaa atcactctac
    52861 acaccttaca agtagaaaaa agtaacctta gtgatgagta ttatataaac ttcttaatac
    52921 atatagctat atgtattaag agaatttcaa ttaaaagata tattaactta tctgaaggtt
    52981 tttttagaga attattagaa catgaatact ttgaaaaatc aaaaatttta tgtgctaacc
    53041 taagtgaagc ttttgacgta gaatttattg atgaagaggt ctgttatata cttcttcata
    53101 tagaaggatt acttaaatca ataaagaaaa ctactactct tgaacctctt aaaaatgaag
    53161 aaattacaga gtataatcta tctgaagcaa tagctgttca ttggggaagt atactaaatt
    53221 taaacttaaa ggatgattta atattaataa agtctcttgc taatcacctt aaatcagttc
    53281 tacacagaat aaactatgga tttactatta caaatcccat attaaatgat ataaagagaa
    53341 catttcctta tacttataag gctgctaaag agtctaatac tgttatcaaa cagttgatga
    53401 attatgaagt caatgatgac gaaataggat atcttgcttt atatttaata tcagcaatag
    53461 atagaagcaa atctccccta aatactattt taatatgtca ctgtagtgat ggtgtatcta
    53521 atttattagt ccaaaagtta agttttgaat ttaatcagat taatatagtt aaatctatac
    53581 ctctatcttc tatttcattt acgaactttg atgatgtgga cttaatacta accactgctc
    53641 ctgtagactt tgaacataat gctgaactga taaatataaa tgctctttta agtaagaatg
    53701 atatttccag acttagtaca attataaaaa aactatattc tgaaaaaaat aaaatattat
    53761 cttataaata aacaaaaata ggcttgtctc aacatagaga taaacctatt tttcattgtc
    53821 caaatgataa aaatatattt gatttcatag aaaaagtgcc tcatgaacta aaaaattcat
    53881 tttgagacac cttcattttt tatatttaat ctttttattt aagcttttat attttttaaa
    53941 cttttatatt ttttaaactt ttatatttaa agtaaatttt attattttta ttgagcgcaa
    54001 gcattattta tattattaat ttcttcagta ttcttaccaa actttgtagt tacaaatcct
    54061 attgttttta ttatgagtaa taaggatatc aatgaatata tagatatcat aaaattctta
    54121 cttattattc cagatgctac tattataaat ccatctagca taaataatat aactggcaac
    54181 ttcagaaact taattacctt attaataagc attgcaacta aatcagttcc accagtacta
    54241 cagcccctta ttattgctat ccctatagca gttcctacca ataacccacc tatcactcct
    54301 gctactaata gattgtcagt tatgtgagtt tgtggtatta cttttaagaa taatggaccc
    54361 ataagagtta tatataatgt ttttattcca aaacctttcc ctaaaaataa taatcccatt
    54421 actaaaagtg gcatagatat tcctaatgat atattctcaa caggcattga taagaatgtg
    54481 ctaagtacga ctgataatcc tgttatccct cctggagcta atgtatgttc tgcaaagaaa
    54541 aaattaactg ctaatgccat taaaaatgct gatattgtta tcagtaaata atcttttatc
    54601 atttcttttt tcatttaata acctcctatg attatattgt ttcagtataa aaaatcaatt
    54661 aaattagtct agcttcctgt agtttttcat aaaattaatg cttccttaaa acattaagaa
    54721 agcatctctt ttatcatagt ttattatctt attctaaacc aaactttgta gctgcttcat
    54781 ctatagcttt tctacaattc ttaacacctg ctgttatacc atctttatcc ttgaatattg
    54841 ctgaagataa tattacaacg tctggattac attctattaa tggctccata ttgtctgctc
    54901 ttagtccacc atctattgct aattcacact taggattctt ttcgtctatc atttttctag
    54961 ctctttttac taaatcaaca gcactctttc tccatcccca gttatctttt ccatcagttt
    55021 catcaacacc atgaacgact atttgcaatc tatctatatc atatatagct tcatcaacaa
    55081 aacatagtgg tgtgtaacat ccaattgtta atcctacttt cataccaaat tctctacagt
    55141 aatttattat gtatgcaaga ggagcgccta aaaaatgttc tgctggtatt atcaacaagt
    55201 tacatcctgc tgctgctatt ttttctataa ataatctatc acaatctctt gtgtatatat
    55261 ggcattctat tggtttatct gtcactggtc ttattgcttc tattatttga tgaccaccca
    55321 taagttgcat attttttaag tcatgcatat ctgctgcatc tgaatgtacg tagtctacac
    55381 ctgcatccat tgcttcttta actacggctg atatatgacc gtaatttaca tgtgctaatc
    55441 ctgctgctat ttttatgtgt ttcatatttg tatacctcca tattttactt taattagtat
    55501 taaatattag tttttgatat tttttaatcc agtattactt ttatttagca ttacttttat
    55561 tgctgtatat atttctttta tgaacttatt ctgcttttgt taactgtgaa cttattttgc
    55621 tttgtaaatt aatattacac tgatatattt tttttcacaa cactttgact actccttaaa
    55681 agtaccttta tattgtggta aattatagac attaattgta tataaaaagt tagtttttta
    55741 ccacattctt atggcattta ttgtctatac taactcaatt gatatagcta gttcatttaa
    55801 tatataatca gactataaat ataaatttac tcgataaaaa gtaccattca taacaatatt
    55861 aatgaaaggg agatgtaaaa tgagaaactg tattattgcc caatctggag gccctacagc
    55921 tgttatcaat gcttctgcag ttggtattta tgaaaaaaat ttagaaacta aatactttga
    55981 taaagtatat tttggtatca atgggattga aggtatatta aacaaaaata taatagattc
    56041 agactcctta aacaaagata atgtattaaa tttaaagcaa acaccttcgt ctggtcttgg
    56101 ttcttgtaga tacaaactgg ctgattataa aataaattct tatgattatg atagagttat
    56161 aaatatacta gatgaatatg aaatagatac attattttat ataggcggaa atgattctat
    56221 ggacacagtg atgaaatttt ctcaatactg caaaaacact acttgtaaaa taaaatttat
    56281 agggattcct aaaacaatag ataatgattt attaggaact gacaactgtc ctggatttgg
    56341 ttctgctgca aagcttattt ctacactaat aatggaaaca tacttagatt tttctatata
    56401 caaaaataat ggtatattta tagtagaaac catgggtaga gatactggat ggttagcagc
    56461 ttcttcttca ctagctagac ttaatgatac tccacttgtt gatttaatat accttccaga
    56521 aattgatttc tatgaagata agtttataga agatgttaga aaaatattta aagaaaaaaa
    56581 taaggcttat atagttgcat cagaaggtat aagggataaa tacgggaact ttatatgcga
    56641 acaaaaaact tctgctcatg ataaatttgg tcattcccaa ttaggtggag taagcaacta
    56701 tctaaagaat cttatagtat catcatcaat tacaacaaga gtaaaaacct tagaacttgg
    56761 tgtaatacaa agatgtgcta tgcatttagc ttcagatact gatttaaaac aagcagctga
    56821 agttggtgca tatgggatag aactatctaa aaaaggatat acaggagtta tggctatcat
    56881 taatagaaca tgcaataatc cttataagta tgaagttagt catactgata tttctaaaat
    56941 agctaacaag acaaaatatt ttcctaaaga gtggataaat gaagaatgta attatataac
    57001 tgatgaagcc ctaaattatc tatatccact tattcaaggt aaaccagata tcaaatataa
    57061 aaatggacta ccttattttt caattattta ctaggaaatt attataattt gatatacact
    57121 caattctatc gctcttaaat acctaagtgg ctgttaaata aaattataaa ttgttatatg
    57181 acagtcactt aatcgtttaa ttatttaact gtttagtttt ttaatttttt aatctttttt
    57241 aatactaata cgatttgttg taataatatc tcacacccat tttcattttg gatagattat
    57301 aattagttag acaataaata ttttgtcatg atattattat atatagtgat acaaatagaa
    57361 atacttttat catgactaga aaaacaaaga ttacacaaag gatttaaaaa tcaaacaact
    57421 tcaattaaaa taaatggtta aaataacttc taaatcaact acataaacaa taaaagaaca
    57481 taaagtagtt aaatctacaa ttataaaatt agtaagacta taataactta aataaaatta
    57541 aagttaacaa agactattaa agttagcaaa tattcctcta cctaaataat tgctttttat
    57601 aaagagttat ttattttaat ttattttaat tgaaatacga ttttaactag aacaatagat
    57661 taatgtaata tttaatataa aataattata ataattgaaa aggaagtaat ctatgatgaa
    57721 aatagcagtc ataacaaccg aatttttaaa agagtttgta gataattcta taaaaaagtt
    57781 aaatattaat gcagaaattg aaatatatat ctatagagat ttttctcatg ttggagattt
    57841 atatttagaa atagaagata ggtttgatgg atttgcagtt agtggcccca tccccaaaaa
    57901 agctattaca aaaaaggctg gaactattaa aaaacctcta
//
LOCUS       DAELSY010000004        55010 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.004, whole genome shotgun sequence.
ACCESSION   DAELSY010000004 DAELSY010000000
VERSION     DAELSY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 55010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55010
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.004"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            83..1063
                     /locus_tag="KPG09_000212"
     CDS             83..1063
                     /locus_tag="KPG09_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0964841.1"
                     /translation="MNLKLIWINILNPVFIKNEIKRKKIQIIIFFFINFGLTYSLGLL
                     LYYNKFIDPENFASFMMILPLSSVAIAKFYTEGLNNDMYEFYSLIILFFLIYSLLIVL
                     KLLNLINNNEFIIINLILTFISSLSIIIYSSNKKNLYPVKNIKLGSLLIIYFMISVIV
                     PTSFILILQGAQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKL
                     GVIILGMCWSLWHLPLIFTLYTPEAPILGVILRSIHIVGISIFLGYLYIKTKNIWFCA
                     IIHVLNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
     gene            complement(1162..2124)
                     /locus_tag="KPG09_000213"
     CDS             complement(1162..2124)
                     /locus_tag="KPG09_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0964842.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIITVMAAFYIFEYYLSTILMEI
                     IRKILIATGDINLKTNYFSEYANWLNDVGLQVVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFTWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(2444..3394)
                     /gene="hpdA"
                     /locus_tag="KPG09_000214"
     CDS             complement(2444..3394)
                     /gene="hpdA"
                     /locus_tag="KPG09_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBG0964843.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFKDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSAENISDIISFMHKNNLIEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDIDEEHLEELQDIFLDNGIACYVGHETAF"
     gene            complement(3387..3644)
                     /gene="hpdC"
                     /locus_tag="KPG09_000215"
     CDS             complement(3387..3644)
                     /gene="hpdC"
                     /locus_tag="KPG09_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBG0964844.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDSACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            complement(3648..6356)
                     /gene="hpdB"
                     /locus_tag="KPG09_000216"
     CDS             complement(3648..6356)
                     /gene="hpdB"
                     /locus_tag="KPG09_000216"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBG0964845.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDHRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            complement(6653..7669)
                     /gene="tsaD"
                     /locus_tag="KPG09_000217"
     CDS             complement(6653..7669)
                     /gene="tsaD"
                     /locus_tag="KPG09_000217"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBG0964846.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSNKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            complement(7675..8151)
                     /gene="rimI"
                     /locus_tag="KPG09_000218"
     CDS             complement(7675..8151)
                     /gene="rimI"
                     /locus_tag="KPG09_000218"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBG0964847.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKCIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            complement(8141..8857)
                     /gene="tsaB"
                     /locus_tag="KPG09_000219"
     CDS             complement(8141..8857)
                     /gene="tsaB"
                     /locus_tag="KPG09_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBG0964848.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            complement(8857..9309)
                     /gene="tsaE"
                     /locus_tag="KPG09_000220"
     CDS             complement(8857..9309)
                     /gene="tsaE"
                     /locus_tag="KPG09_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBG0964849.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            complement(9448..10488)
                     /locus_tag="KPG09_000221"
     CDS             complement(9448..10488)
                     /locus_tag="KPG09_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG0964850.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWEDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            complement(10817..11422)
                     /locus_tag="KPG09_000222"
     CDS             complement(10817..11422)
                     /locus_tag="KPG09_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0964851.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     regulatory      complement(11547..11664)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(11724..12890)
                     /locus_tag="KPG09_000223"
     CDS             complement(11724..12890)
                     /locus_tag="KPG09_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0964852.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     gene            complement(13038..15179)
                     /locus_tag="KPG09_000224"
     CDS             complement(13038..15179)
                     /locus_tag="KPG09_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBG0964853.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYNEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            complement(15323..16430)
                     /gene="prfB"
                     /locus_tag="KPG09_000225"
     CDS             complement(join(15323..16354,16356..16430))
                     /gene="prfB"
                     /locus_tag="KPG09_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBG0964854.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            complement(16445..19120)
                     /gene="secA"
                     /locus_tag="KPG09_000226"
     CDS             complement(16445..19120)
                     /gene="secA"
                     /locus_tag="KPG09_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG0964855.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMVREDLPDSVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            complement(19252..19803)
                     /gene="raiA"
                     /locus_tag="KPG09_000227"
     CDS             complement(19252..19803)
                     /gene="raiA"
                     /locus_tag="KPG09_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG0964856.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDSEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            complement(20356..21030)
                     /locus_tag="KPG09_000228"
     CDS             complement(20356..21030)
                     /locus_tag="KPG09_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBG0964857.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDIGVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            complement(21033..22085)
                     /locus_tag="KPG09_000229"
     CDS             complement(21033..22085)
                     /locus_tag="KPG09_000229"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBG0964858.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISKKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKTPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSNTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            complement(22133..23092)
                     /locus_tag="KPG09_000230"
     CDS             complement(22133..23092)
                     /locus_tag="KPG09_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBG0964859.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIVDFTFSVV
                     TKEESPAVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            complement(23188..23706)
                     /locus_tag="KPG09_000231"
     CDS             complement(23188..23706)
                     /locus_tag="KPG09_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBG0964860.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNEKEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            complement(23717..25051)
                     /gene="celB"
                     /locus_tag="KPG09_000232"
     CDS             complement(23717..25051)
                     /gene="celB"
                     /locus_tag="KPG09_000232"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBG0964861.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLISSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIVTPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            complement(25090..25416)
                     /locus_tag="KPG09_000233"
     CDS             complement(25090..25416)
                     /locus_tag="KPG09_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0964862.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            complement(25441..25749)
                     /locus_tag="KPG09_000234"
     CDS             complement(25441..25749)
                     /locus_tag="KPG09_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0964863.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            complement(25890..27803)
                     /locus_tag="KPG09_000235"
     CDS             complement(25890..27803)
                     /locus_tag="KPG09_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0964864.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNVSTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            complement(28135..28917)
                     /locus_tag="KPG09_000236"
     CDS             complement(28135..28917)
                     /locus_tag="KPG09_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBG0964865.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(28920..31139)
                     /locus_tag="KPG09_000237"
     CDS             complement(28920..31139)
                     /locus_tag="KPG09_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBG0964866.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(31403..32500)
                     /locus_tag="KPG09_000238"
     CDS             complement(31403..32500)
                     /locus_tag="KPG09_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964867.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSNIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(32816..34009)
                     /locus_tag="KPG09_000239"
     CDS             complement(32816..34009)
                     /locus_tag="KPG09_000239"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBG0964868.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(34146..34249)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            34438..34947
                     /gene="yyaC"
                     /locus_tag="KPG09_000240"
     CDS             34438..34947
                     /gene="yyaC"
                     /locus_tag="KPG09_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBG0964869.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(35058..35486)
                     /gene="fabZ"
                     /locus_tag="KPG09_000241"
     CDS             complement(35058..35486)
                     /gene="fabZ"
                     /locus_tag="KPG09_000241"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBG0964870.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEIEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(35502..36521)
                     /locus_tag="KPG09_000242"
     CDS             complement(35502..36521)
                     /locus_tag="KPG09_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG0964871.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(36718..36978)
                     /gene="spoIIID"
                     /locus_tag="KPG09_000243"
     CDS             complement(36718..36978)
                     /gene="spoIIID"
                     /locus_tag="KPG09_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBG0964872.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(37076..37744)
                     /locus_tag="KPG09_000244"
     CDS             complement(37076..37744)
                     /locus_tag="KPG09_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBG0964873.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFNDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(37810..38874)
                     /gene="spoIID"
                     /locus_tag="KPG09_000245"
     CDS             complement(37810..38874)
                     /gene="spoIID"
                     /locus_tag="KPG09_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBG0964874.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKSTVSYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(38961..40214)
                     /gene="murA"
                     /locus_tag="KPG09_000246"
     CDS             complement(38961..40214)
                     /gene="murA"
                     /locus_tag="KPG09_000246"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBG0964875.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(40235..40972)
                     /locus_tag="KPG09_000247"
     CDS             complement(40235..40972)
                     /locus_tag="KPG09_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBG0964876.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            complement(41435..42730)
                     /locus_tag="KPG09_000248"
     CDS             complement(41435..42730)
                     /locus_tag="KPG09_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBG0964877.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKMQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKRAVDFSANWLNVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSITPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSNSVSLEEYRNIDNTMRNKSEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            complement(43183..45015)
                     /gene="glmS"
                     /locus_tag="KPG09_000249"
     CDS             complement(43183..45015)
                     /gene="glmS"
                     /locus_tag="KPG09_000249"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBG0964878.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEEEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            complement(45282..46628)
                     /locus_tag="KPG09_000250"
     CDS             complement(45282..46628)
                     /locus_tag="KPG09_000250"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBG0964879.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILNGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            complement(46798..47355)
                     /locus_tag="KPG09_000251"
     CDS             complement(46798..47355)
                     /locus_tag="KPG09_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG0964880.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            complement(47356..48108)
                     /locus_tag="KPG09_000252"
     CDS             complement(47356..48108)
                     /locus_tag="KPG09_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG0964881.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            complement(48108..49187)
                     /locus_tag="KPG09_000253"
     CDS             complement(48108..49187)
                     /locus_tag="KPG09_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG0964882.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            complement(49216..49431)
                     /locus_tag="KPG09_000254"
     CDS             complement(49216..49431)
                     /locus_tag="KPG09_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0964883.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            complement(49474..50151)
                     /locus_tag="KPG09_000255"
     CDS             complement(49474..50151)
                     /locus_tag="KPG09_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0964884.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            complement(50141..51220)
                     /gene="buk"
                     /locus_tag="KPG09_000256"
     CDS             complement(50141..51220)
                     /gene="buk"
                     /locus_tag="KPG09_000256"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG0964885.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            complement(51242..52144)
                     /gene="ptb"
                     /locus_tag="KPG09_000257"
     CDS             complement(51242..52144)
                     /gene="ptb"
                     /locus_tag="KPG09_000257"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBG0964886.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            complement(52448..53629)
                     /locus_tag="KPG09_000258"
     CDS             complement(52448..53629)
                     /locus_tag="KPG09_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964887.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNIDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            complement(53626..54465)
                     /locus_tag="KPG09_000259"
     CDS             complement(53626..54465)
                     /locus_tag="KPG09_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBG0964888.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
BASE COUNT        16646 a        10367 c         5824 g        22173 t
ORIGIN      
        1 tattgactgt catgaattta taatattcaa taaaatattt taatttagtt aagaatctat
       61 attgtaaagg agtattaaat taatgaactt aaaattaata tggataaata ttctaaaccc
      121 agtatttatt aaaaatgaaa taaaaagaaa aaaaatacaa attattatct ttttctttat
      181 aaactttggt ttaacttatt cattaggttt actgttatat tataataaat ttattgatcc
      241 agaaaacttt gcatcattca tgatgatatt acctctatct tcagttgcta ttgcaaagtt
      301 ttatactgaa ggattaaata atgatatgta tgaattttat tcactaatca tattattttt
      361 tcttatttat tcattattaa ttgttcttaa gttattaaat ctaataaata ataatgaatt
      421 tataattata aacctaatat taacttttat aagtagctta tcaattataa tatattcatc
      481 aaataaaaaa aatttatatc ctgtaaaaaa tattaaacta ggttctttat taattattta
      541 ctttatgatt tctgtaattg ttccaacatc atttatacta atactacaag gggctcaacc
      601 taattatagt ggtatcataa aatttatatt tactccaata acatcattaa ctactattta
      661 tatttttttt agtgaagaat atgcctggag aggtttttta cagaatattt tttttgataa
      721 atttggtaaa aaattaggag tcataatatt aggtatgtgt tggagcttgt ggcacttacc
      781 actaatattt acattatata cacctgaagc gccaatatta ggagtaatat tacgttcaat
      841 acatatagtt ggaatttcaa tatttttagg atatctttat ataaaaacta agaatatctg
      901 gttttgtgct ataattcatg ttttaaataa tagtgctttt cttatcacaa attctaaaga
      961 atcaactatc acttaccatg atatactaat agtatctatg gttgttttaa ttttttatgt
     1021 tccatttcta tttacaaaag aatataaaaa taacttggag taaatagaat atccaagtta
     1081 tttttatatt tttgtaaatt tccaacaatc ctacagaata ctttaagttt agaagatatt
     1141 attaaaattt attataatca actaattctt gatattagtc acatttactt tatcactaac
     1201 aaaatcatac ttagaatctc tatagtaata ccatacaaat ataaagctaa gtagattaac
     1261 aaaagtagtc aaaattccac cttcaatacc aaacatccct ccatttaata gattaccatg
     1321 agtgatttgt gtagttataa tccctggaac ctgaagtcca cttacaggca taccatatat
     1381 aatcccctga aatacattcc atgtaaaatg ataacctata cacatccata tattcgacga
     1441 ctttataaac atatatgaaa aaagaatccc aataattata atattaatta cagacaaaaa
     1501 agtaacatca ggattccata tatgaatcaa cccaaaaata acagatggaa caatcattat
     1561 aaaataaata tttttacatc ttcgtaaaac tgccataaaa aaaccacgat ttaaaatttc
     1621 ttctgcaaaa gcaactaaaa taaagacaag tatccaagaa actgttaaag gtgaaatttt
     1681 tggtacccat gaagtaacat gaccaccacc aattgttatt gttataataa agattactgt
     1741 acaattaaat attcctaata acattcctac atatccttct tttttcttaa ttctataaga
     1801 atgtaatccc atttcactaa taggattttt cataataaat ctccatacaa ttataggaat
     1861 aataatcata agtgaatctg ttagaatttg aaatacaacc tgtaacccaa cgtcatttaa
     1921 ccaattagca tattccgaaa aatagtttgt ttttaaatta atgtcacctg tagcaatcag
     1981 aatttttctt attatttcca taagtatagt gcttaaataa tactcaaata tgtaaaaggc
     2041 agccataaca gttataataa tccaccctga acgtactttt ttgtctttat tcttaataat
     2101 caagttactc catgatttgt tcatatagat gtcttcctcc taactattat ttttagttgt
     2161 ttctaaaata tattctatta aatttcttca tagtagtatt ttaccacaaa acttctgctt
     2221 caataaagta aacgtaatat attctctaac tgtaatttaa actataaaaa atatataaag
     2281 tataaattta aagtaatatt tatggacata aagcaatatg aacaatatat aaaacatata
     2341 aaactttact taattatttc tacataaaaa ccacataaaa aaggatgtaa ctctagcttt
     2401 tagtgtaaca tcctttttta attaaaaaaa cttgtatttg attttaaaaa gctgtctcat
     2461 gaccaacata gcaagctata ccattatcca aaaatatatc ttgtaattct tcaagatgct
     2521 cttcatcaat atctccctta tcagaatact catactcctt acctaattga atccactttg
     2581 attctcctag cctatgaaat ggtaataagt taatctctat taaattattt ttgtgcataa
     2641 aagatataat atcacttata ttttcagcac tatcattaaa cccagagata actggtactc
     2701 taagtactaa ccttccattc cagtcagaat ttgcaaggtt tgatatattt tctaatatta
     2761 aatcattata tacacctgtt tgttctttgt gtttttctct atccatatgc tttatatcaa
     2821 taaatgcaaa atcaatatct ttaaatattt tattaaaaac ttcatttgat acacatgcac
     2881 ttgtttcaat ggcagtgtgt atatttacct catgacactt taataatact tcatgtaaaa
     2941 attcatgctg aagtagtggc tcacctccac taaatgttac tcctccatta cttctccaat
     3001 tgttagaatc acgtttaatt acctgcacaa gttcatctac agtataaggt tttgcacata
     3061 atttaaacgc attataataa catgagttga cgcattcaaa actttcacag tccttacaga
     3121 tgttccaatc tataactggc ttattatcaa gattaaagct caatgcacca tttttacact
     3181 taccatgaca tacagtacat ccattttcat attgacaaga taattcacta aacatcatat
     3241 gtggtcttac agtccaactt tctggatttg cacaccattt acaactcagt ggacatccat
     3301 ttaaaaatac agttgttcta caacctggac catcatgaac tgaaaagctt tgtacatcga
     3361 aaatcatgcc ttctaattgc ttttgactac tcattaaaca catgtccttc acaagtaatt
     3421 gcattcaatg ttgaatatac ccagtcttct ttctctaggc caacacattt gcctatacct
     3481 tcatcattag cttcaacaaa atttttacaa tttttacatt ttggcattac ttttatctct
     3541 ggacatgctg aatcatctaa attaatcatt tgttttgata atctacaaat tcctttggtt
     3601 gcatctactg cacaaaaatt catacaatca ctatgctttc tcatatttta caccccttca
     3661 tactctgttc tagcaattac ttcatcttgt attggtttac ctaattcaca ccaatattga
     3721 gtaaacccag caacacgtac cattaattga cgataattgt caggattttt ttgtgcatct
     3781 tttaatgttt ctgaatctac aacattatat tgaatatgga acccaccttt tcttaaataa
     3841 cttcttgtta aatctaataa ttttttagtt ccttgttgtc cttttatagt agttggatga
     3901 agttttaaat tcatctgaga attttgaact actgcttggt cccaaatagt tgcagattca
     3961 aataatgcat atactccatt tttatctgtt cctgcatatg cagagactga accatctgaa
     4021 tatgttgttc cacttagtct accatctgga gtagccaagg tagcagctcc ctgtggtcca
     4081 tgtgtagaaa cagatatttg gcaagggtac atcttcttag catataaaga ttgtgcttct
     4141 tcacatactt ttgataacca tacctcatag tttttaagaa tattatctgc atacaaatca
     4201 ttatttccat atttaggtgc atcaagacag gccttgtaaa tagcatcata tttaccagtc
     4261 ttatctactt ttacttggtc tgccatagaa taatttccta cttctaatgc atctttaaat
     4321 ccaaaattat ttaatattgc atccttcatt tcctctattg taaatgcttt gtcatcatat
     4381 acaagtttct taagtgctgc aaatgaattg accatagtaa cagtaccaca agtttctacg
     4441 tttaatgttg cattataacg atatcccata tttcctatat gttgaccttt gtctaaacag
     4501 tctggcttta gtagtgaatt tattatagaa gtatcaaatt ttctccatat atctaactca
     4561 atgttatttg ctctttctaa aacatttatt gcttgtttat aataatcttt aaatacttct
     4621 atgacttctt cataagtatc taatggctta ttatgaggct caaatacttg tatattcata
     4681 cgatggtctt taccattcat aagaacaagt tctaaaattt tgggattagc tataaagtga
     4741 actccagagc cagcagattg acctgctcca cctgcaatag agtacgtttt tccgtttaaa
     4801 gttaactgtt tccaacaccc tggagacgtt tccaaacatc cacctaatgc aaatgctctt
     4861 gcttcttcaa cagtcatacc ttcatcagca aattgtttca tcataaatgt tgttccattt
     4921 gagttgttta cccaggctgg ataaccagaa ccaagctttg tacattcagc agctttcatt
     4981 aagaaatctt ctggaagctt ttcatcatat agcatagtta aactaggttg aggagttcta
     5041 catctcatac ttgcttctaa taataactct tctagttcat tagctcctgt agaaccatct
     5101 tcttttaatc cacctataga taaagtatta aatgtattac ctgatagtac tcctccattt
     5161 acgcccgctg aagcaaaaca gtcaatacaa gtgattttta ttctataaag ctctaataat
     5221 tcgataacct cttcttttgt aattagtcct ttttctatat cttgttcata ccaaggatat
     5281 aaaatttgac caaaacgtcc tatagacata ccagatatag catcctcatt tactgatgct
     5341 atatgaattg tataagtcaa ctgaagagct tccctaaatg tacgaggttg tttatgtgca
     5401 atatgttcta atatctccac taaatctgag tattcttttt tagcttctaa atctgtctct
     5461 atactttcta aatattttgc atgttttgca taatttaaaa tccaagtttg taatccttca
     5521 ataacttgaa taacagcctc ataaaaatat agacggtcca taccaataag cccatctcca
     5581 gatgcatttc ctgcaactgc ctttttacat tcttttgcca tttctataat tccatctaat
     5641 ccataattta aaggataaaa ataatttata acttctctac cttgaggaag tgtataccct
     5701 gaatcaaaca tgcatataag tgtactcatt aggttttctt ttaaatcata atctggcatc
     5761 attttttcat agtgaaaacc taaatcttca acagactttc caacccattc ctttgccatt
     5821 tttacaagta caggtacttc ttcttttctc attccgaatt taccagctat tgaaacgaca
     5881 tttccaaaac tttcagtaac atttcctcca ccagacccaa attttgtaag ttcatcagct
     5941 gtgttactgg ctaatttctt tgcctcttct ctcatttgtt ctccttgagc aacaaagaaa
     6001 ctttcactta cccatggcat tggaaaagaa cctctataat ggcgtgtctt ttgcattact
     6061 attttctcac caggtattat atttggtgtc atatgtgaaa aagcagcttt aagagcttta
     6121 gctcttctaa ctactggtat atcaccatct gattttctat attctctatt ataccaatat
     6181 ggaaattcca tatcagcagt agataatgta ttataatata catctaataa gtcttctgct
     6241 cttttacttg cttttttttc attaaattca cttaaatttt ctttattttg aaaatttgat
     6301 ttaatatttt ttgcttccaa aatacttctt attttgtctt ctttactttg actcatttct
     6361 tcccctcctt aatctttcta taattagtat aaaacttttt aagaatgaaa aaaaagggta
     6421 tattacccac tttttaaaat aatctttcta tagaaaaaat taaacttgaa gcaaatctaa
     6481 gccaaagaca atctgtatat cctaaaattt gttgcaattc attggaatat aaagattata
     6541 aatagtttta cttcaagttt taatatatac tatatttatt tttattatat ttaatttcac
     6601 ttctacttac ttttttgaaa caatactttt atatttacaa attcatctaa ctctactgat
     6661 ttatctttaa atttggaact gcatttaaac tcatatcatg cgttttccca tttataaaat
     6721 tataatatcc tgcacaccct atcatagctg cattatctgt gcataaaatt agaggaggat
     6781 acttaacagt aataccattg tctttagcaa gctctgttat tttagctcta agccccgaat
     6841 tagaagcaac cccaccagaa agagttatga tattgtatcc tttgtctttt actgctttta
     6901 gtgctttatt agataaaacc tctactacag cctcttggaa agatgctgct acatcttcaa
     6961 ctactatttc ttcatttttc attctttttc catttagata atttaataca gaagatttta
     7021 atccactaaa actaaaatca tagctatctt cttctaaata ggctcttgga aattctatag
     7081 catgtttatt tccattcttg gccagattat caataatagg acctcctgga tagcctaaat
     7141 tcatagctct tgaaatctta tcaaatgctt ctccagatgc atcatctctt gtttttccta
     7201 aaatttcata cttgccataa tcttttactt caaccaagtg agtatgacct ccagatacta
     7261 ttaatgttat aaaaggaggt tttaaatcct tatgctctat atagtttgca cttagatgcc
     7321 cttctatatg atttactcct acaagtggaa tattcaaagt atatgcaagt gctttagcat
     7381 aagatagacc aaccaataaa gcacctacta gcccaggtcc atatgtaact gcgatatggt
     7441 ctatatcatt aaaacctata tttgctttgt ctaaagcttc ttgaacaact atatcaatat
     7501 tttccacatg ttttcttgag gccacttctg gaactacacc accaaatttt ttatgtgtct
     7561 cgatttgagt agagattatg tttgaaagta cttctctacc attttttaaa actgatgcag
     7621 ctgtttcgtc acaactactc tctatagcta atgttattat atcactcata aactctacac
     7681 ctcttttaat tggttccaca ttataattgc atcttcttta ttatcactgt agtattcttt
     7741 tcttattcca cccattttaa atccatattt tctatataaa ttttgtgcta ttttatttga
     7801 tgctctaact tctaatgtca tagatgcaat attattatct ttacataaat caactatgca
     7861 ttttatcaac ttgtccccta tttttttccc tctatagtca ctatgaacag ccacattatt
     7921 tatatgccct tcatcaagta taagccaaat tcccacatat ccgacaattt ttccatttaa
     7981 cttagctact atataccttg ctacttcatt agacaactct tttctaaaag actcttttga
     8041 ccaataatct tcaaaacaat ttttttctac ttcaaaaact tcatctatat cttctgtagt
     8101 catttcttct atttttatat catctactat tagttttgta ttatcttcca ttatttagcc
     8161 tcttcatttt ttcatcatat tgtacttcag cttgagattt tctgatatac ataggattta
     8221 tcgtataaca agtatgtaca tctatatttt tattatattt atttaatgct aaagagcata
     8281 agcttcctgc gttagataca ttattagctg gtgaaggtat ttttatattt tttatattct
     8341 ctaacttgtc tttatatttg tatacagctt ctccaactat tataaattct tcattagttg
     8401 atacaatttc atctactaaa gcatcaaact ctacaacatc aacaccatct aattctatca
     8461 tctctccatt ttcaaacttg tatttagctg aataaacttg atttttctgc gcatctaata
     8521 tacaacatat ctttctatca cagaaattta tgttatttgc tagagattcc aaagaattta
     8581 ctgctattat tggtatatta tttacatggg ccatagcttt tactgtagcc atacctattc
     8641 taagtcctgt aaacgaacct ggacctatac atacagctaa taaatccata tcttttatac
     8701 ttaaatcact catactcaac atattttcta tcattggcat aagcttttgt gaatgagttt
     8761 ttttgttgtt tactgtaaac tcacatatta agttatcatc ttcaacaact gctacacttg
     8821 ctgccataga agacgtatcc atacctagta ttttcattat ttaatcaact cctcaactat
     8881 ctctttataa aactctcctt ttggagttaa tatcatttct cttccttcat ctttatatct
     8941 caattctata ttcaaatatt cttttggaag tatatcttct attagattag cccattctat
     9001 tatacagatt ccatttgaat ttacatattc atcataacca atatcataca tttcatcact
     9061 actacctatt ctataaacat caaaatgata caaaggtatt tttccttcat attcatttat
     9121 tatagtaaaa gtaggacttg ttatataatc ttctatccct aatgaatctg ccaagctttg
     9181 tgtcattgta gttttaccag ctcctaaatc tcctactaaa cagataacac tgccttcttt
     9241 taaaagttta cctaatttat atcctatttc tctagtttta ttttcatttt ctaaatatat
     9301 ttttgccatc aattcatctc cattttgata taatcgtcat gtgaatttat atcactgctt
     9361 aattaattat agtataagcc tagattatta taaagtactt gtctttatct ttttttatct
     9421 ttcactattg atggctgtat ctacacctta tatacaatca tcaatagcaa tttttacata
     9481 ctcttttgct ttatggtttt gcttcaatgc ttctttacat attctctcag attgctcttt
     9541 tacatactta agagacttat agttctcttt tactgcttca atacacattt tttctgtttg
     9601 attttttata tacataagca tttttccatt ttgtcttaca atctctatac atatatcttc
     9661 tgtatactta tctatgtaac ttagtacatc tttaatatta tattttttat ttaaatatct
     9721 tatagcattt atacatattt cttctgtctt attgttgaca aattgtagac ttcttccatc
     9781 ttgtttgaca gcttctatac atattttatc agtttgttta tttacatatt gtaatgccat
     9841 accattttgt cttacggatt ctatacacat tttttccgtt tgatttttta catattttaa
     9901 tgctttataa ttttgcttaa tggcctctat acataattct tcactctgtt ctctaacgta
     9961 tgcaagacac ctgccatctt ttttaattgc ttctaaacat atccttcttg tttgagtctc
    10021 taaagccttt agtatactta caacatctat attggaagaa tgacttattg cttttataat
    10081 aagcccttct gttttatttc gtacatactt aagtgcatta caatcttttt ttatagcttc
    10141 aagacacaag tattctgttt gttcttttac atattcaagt gctttataat tatactttat
    10201 tgcctctaaa cataattctt cggtttgatt ttttacatac tctaatgcct tgtaattctg
    10261 ccttattgct aaaatacaca taccatcagt ttgattttct atatttttta attcacgata
    10321 atcgtatttt accctttcca tattttcaat atattcatta gtgatatact ttaaagaatc
    10381 tcctaatttt gtatcataaa taccaatatg aatatcttcc caattactat taaaataata
    10441 ccctttatca tctaaaaata atttttttat ttttttagat aaatgcatga atatacaccc
    10501 ctatcattat taaattagtt attattaaaa gtttgattat tagtctttat agttttttaa
    10561 taataacttt attttttgtt tattataaat tcgctctgta tattgtagat acctttttca
    10621 tattttaata gttaatcgtt ttaattcttc ctttatttta aaatttaaat ttgtctaaat
    10681 atttcttatt ttatatacat tttttgataa attaataaag ccataatata tttaatatat
    10741 caatcacaat ataggactga agttataaat aaattatggc tattcaatac atttaatttt
    10801 atattttctt gtctaattat ttctttatta tatttcccat ttttttataa agtggcaatg
    10861 ttactaaaga tattataaca gccttcagca cattaaatgg agctatcatc caaattacaa
    10921 aagtgaataa atcatgaact tgaggattta tagctgaacc taaacctatt atagcatcta
    10981 gtcccataag ttgaccataa agtggcaaca ttacaaaata atttactaat gaaccaatta
    11041 tagtcataga tattgttcct aatacaaaac caatcagtac accttttaaa tctccacttc
    11101 tcttatatga ataacaaagt atcattacat aagaaccacc aattaatata tttgcaaatt
    11161 ctcctattcc tccagttgtt gttgctgtta ttgcttgtaa cacatttttt actacaacta
    11221 ttccaaatcc aactaatggt cctagagaca ttcctccgaa tattgctgga atatctgata
    11281 agtcaatttt taagaaatct ggaaatatac ctggtattgg tgcttgaata aacattaata
    11341 aataagatat tgcagataaa attgacatta ctactaaagt tcttgttgaa atcatattgc
    11401 ctctttttgc tgtgttttgc ataattattt tccccctaat caatatgttt atatttttta
    11461 ggaaaaacaa aaaactcgaa gaccctatct tcgagttttt atggatatag tttaaacaaa
    11521 ctaaaataaa cttatttatc ttccataaat atcttctctc atccagactt tactgtcggc
    11581 tttggaatct caccaaatca accacctaag tagttcgcgg gctataccgc cggtagggaa
    11641 tttcaccctg ccccgaagat cttctttgat tttttaattt tccttacact cttatattag
    11701 ttaatttttt aataattgtc aagttatttt ttctcataca ctactttgcc atttattata
    11761 gtgtaaagta cattactttg aatctctagt ggagaattat cccaaattac taggtctgca
    11821 tctttaccaa cttctataga gcctactcta tcttctattc ctaaagtctt agctggattt
    11881 atcgtaattg attctattgc tttttcttct ttcataccat gttttactgc aatacctgca
    11941 catattggaa gatattgaac tggtattact ggatggtctg tcattataca tacatcaaga
    12001 cctgcatttg aaagtattcc tgctgtatta aatgtcaaat ttctaagctc aaatttagac
    12061 ctttctgaca aagatggccc tactattaca ggaaaccctt cttccactaa ttcatctaca
    12121 ataagatgac cttctgtaca atggtctaat gtaagcttta aattaaattc ttttgcaatt
    12181 cttattgctg taaacatatc atctgctcta tgagcatgtg ccttaaaagg aatttctctt
    12241 cttaaaactg gtattaaact ttccattttt atatcatatt caggtttttc acagtcttca
    12301 tcatcatgac tctcatacat atcaatatcc tctaaatatt cttctgcttt ttttaggttt
    12361 tctctaagta aagcagctat agccattcta gtttgaggag atttatcatc ctgaccataa
    12421 cagctctttg gattttctcc aaaagcaatt ttagatgcaa ctggattttt tattaccatt
    12481 ttatcaattc tttttccaca tgtctttata gctatacatt gaccacccat aacatttgca
    12541 cttccagggg ttgtacaaac agaagttatt ccaccttcaa aagcttcttt aaaagttcta
    12601 tccattggat taattccatc tataggattt aattgtggtg ttattgggtc agtctcttca
    12661 tttccatctg ctccttcaaa ccctatacca tcttcccata aacctaagtg agtatgagca
    12721 tctataaatc caggaaacac aagtttatta ctagcatcta ttacctctac atctaaagga
    12781 gctatcaaat cttctccaat ttctattatt tttccttcat ctataagtat atctccatga
    12841 attatcccat ttgttatggt atttatttta ccatttttta taaaaatcat tcattttcct
    12901 cctgattttc aagactatcg ttcttatttt aatatatttt cttactatat acttttacaa
    12961 atttttaaca tttatacact cgagtaatct catttacaaa tatagaattt aagtactata
    13021 accatacata aacttatcta ttttttcatt gataaggcaa ctcttttttt gtttaagtca
    13081 attccaatga cttttacatc tactatatca cctactgtaa caatactcat agggtctttc
    13141 acaaaactat tactcatttc tgatttatga actaatccgt cattttttat tccaatatct
    13201 acaaatgcac caaagtctac aacatttctt attgtacctt ttaaagtcat accctcttgt
    13261 atatcttcaa tttttaagac atcagttcta agtataggtt ttataccttc ttctcttggg
    13321 tctcttcctg gttttttgat ttcagctatg atatctttta acgtaacttg tcctacttcc
    13381 agtttttcac ttaattccct caagccaatt tcttttattt tttcatctat ttcacctata
    13441 ttcttattct ttacatcatc taaagaatat ccaattatct caatcatttt tttacatata
    13501 tcataacttt caggatgcac acctgtatta tctagtggat ttttaccctc taagattctc
    13561 ataaatccag cacattgagt aaatgcttga ggtcctagtc tttttacttt tttcaattgt
    13621 gctcttgatg taaaatctcc attttcttct ctatatgcta ttatattttt tgctatagct
    13681 ttacttattc ctgcaacatg ttctagtagg gagtatgatg cggtatttaa atctactcca
    13741 acactattta cagaatcttc aacaactcca tctagcactt cttctaatct ctttttgttt
    13801 aaatcatgct gatattgacc aacaccaata cttttagggt ctatttttac aagctctgct
    13861 aatgggtctt gtaatcttct agctatagat atagctcctc ttattgatac atttatatct
    13921 ggatgttcct catttgcaag ttcagatgct gaataaacag atgctcctgc ttcactaact
    13981 atcacatact gcacttcgct atctatttct tttatcattt ctgatacaaa agtttctgat
    14041 tctctagatg cagtaccatt tccaattgaa attatatcta tatcgtattt ttcaatcaat
    14101 ccttttaaaa ctttctttgc tccctctaca tcattttgag ggtctgttgg atatactgtt
    14161 gtataatcta gtagtttacc atttttatct actacagcta ttttacatcc agttctaaat
    14221 gcagggtcaa atcccataac tactttgtct ttaactggtg gttgaagcaa taaactttta
    14281 acattctttc caaatacact tatagctctt tcttgagcta tttctgttaa atgatttctt
    14341 atttctcttt ctattgatgg gaaaattaat cttttataag aatcttctat tgaacttacg
    14401 atttcttctt tatttttgaa atttttatca tttacatatt cgtttattat gtaatttaaa
    14461 actttatcat tatttatttc taacttaacc tttaggaagc tttctttttc tcctctgttt
    14521 attgccaaaa ctctatgtgg agccatactt tttactgctt cgttataatc ataatacata
    14581 tcataaaccg atttctcatc agtagcactt ttgctaacta tcataccttc tcttaaagct
    14641 aattctctta tatactttct aatcttagca tcatctgaga ctagttctgc aattatatct
    14701 ctagcacctt ttagagcatc ttcaatagaa agaacttctt tctcttcatc aagataattc
    14761 tttgcctcta tttctatatt atctaaatta ttctctagta ttgacaatgc taaattttct
    14821 aatccttttt cttttgctat tgttgctcta gttcttttct tttgtttata tggagcatat
    14881 atatcttcta cttcttgtaa tgtttttgct ttttctatat tttgtgttat ttcatctgtt
    14941 aatttccctt gttcatctat tagtctaact acatcatctt ttctactttg aagatttctc
    15001 aaatacatta atttttcata aaattctctt agtgtaacat cactcatttc gccagtcatt
    15061 tcttttctat atctagctat aaatggtatt gtatttcctt catcaataag ttttaatgta
    15121 ttatttattt gttcatctct aagattaaac tcttttttta agatttgatt tatatccaat
    15181 ttattcactc ctaatgttag tttttcataa gtataacaaa acagaacgaa ggctgagggt
    15241 tatcgtcctc agcctaagtg ctgtaagtgc atattaagca ctaaccttct tatacatata
    15301 tcttaattac agtgcatatt tattaaacta ttttattcat ttttaagtat tcattaataa
    15361 acaagtctat atttccattc attacactat ctacatttcc aaactcagca tttgttctat
    15421 ggtccttaac caatttatat ggttgaaaaa catatgacct aatttgacta ccccaagtta
    15481 tttgtgagta tttgccttgt atatcttcta ttttttcctt ttgctctaac tcttttaatt
    15541 ctatcaattt cgccattaaa agtctcatag ctctgtcctt atttagatgc tgagaccttt
    15601 cattttggca ctgaactact acaccagtag ggatatgtgt tattctaact gctgaatcag
    15661 tagtatttac atgttgccct ccagctccag aagctctata tgtgtctatc tttaaatctg
    15721 acggattaat ttctacttct atattttcat ctaactctgg tgttacatct atagatgcaa
    15781 atgaagtatg tcttttccct gatgggtcaa atggagatat tctcactaat ctatgaactc
    15841 ctttttcact ctttaaatac ccataagcat ttgttccttc tacaagaatt gtagctgtct
    15901 ttatccctgc ttcagggtct gatattatat ctaaaagctt aactttataa cccttacttt
    15961 ctgaccatct tatatacatt ctcaaaagca tttgagccca atcttgagca tctaagccac
    16021 cagtaccagc atttatagat aatatagcat tatttttatc atactctcca tttagaagag
    16081 tctttatttt catttcagat aatttttctt ccattgattc tatggacttt tctatatctc
    16141 tttctacaga atcatcatct tcttctaaac ctatttcaat taatacttct atatcttcta
    16201 ataagctttt taagctttca tattcttcta atgtctcttt taaagattta ttttcttgaa
    16261 gtactctttg agcgacttca ttatcattcc aaaagtcttg ttgattcatc ttttcttcat
    16321 taatttgtat atttttcatc aatgaagcta tgtcaaagag aaaccctcaa ttcttctaaa
    16381 tttgaagcca tctgctccat actatgtcta tactcttgta actttaacat ttaatcctcc
    16441 gagattatct tccacaacag tttttatatt ttttaccact tccacaaggg cataagtcat
    16501 ttcttccaac cttttcatct tttttaacag ttttacttgt tggaagtgtt ccatctgatg
    16561 gagaagatag attttcaaca tctacaactg cttttctttc cactggagtt tctattgtta
    16621 tattaaataa atatcttaca gtgtcttctt tgatatgttt attcatttca tcaaacatat
    16681 caaaaccttc cattttataa gcaataactg ggtcttgctg acctacagca cgaagaccaa
    16741 taccttggcg taattggtcc atagcatcta tatggtctat ccaatggtta tcaacagctt
    16801 gaagtaagat tactctctca acttctctca ttctctcata gccaacttgt tcttcttttg
    16861 aagtatatat cttcatagct atttcataaa ctttttctgt tatttcaact ggattaagtt
    16921 tttctatttc tggtatttct atgctacctt ttggcaagaa cagattatac atatgttctt
    16981 tgaatccttc ttcatcaaat cctttatctt gtgtataaag agtaactgct tcttctatta
    17041 tagaatgagt cattgattgt atttgttctt gtaaatcttc accttctaat acacgttttc
    17101 tctctgcata tattatttct ctttgtttat tcataacatc atcatattga agtacatgct
    17161 ttcttattcc aaagttttta ccttcgactt tcttttgtgc tccttcaata gacttagata
    17221 atattctatg ttctatcggc atgtcttctt caagacctat tttatctaca ataccagata
    17281 ttctatcact accaaacaat ctcataaggt catcatcaag acctatatag aatcttgaac
    17341 tccctgggtc accttgacga ccagcacgac ctctcaactg gttatctatt cttcgagatt
    17401 cgtgtctttc tgtacctatt atagcaagtc cacctgcttc tacaacttgt ttttgttctt
    17461 cttgtgtttg ttgcttaaac ttttcaaata gtttttcata ctcttctcta gcttcaaata
    17521 gaatttcatt accttttaca ggtatacctt ctattggagt atctacccta tttactattt
    17581 cctctttaaa tccatttctt ctcatttctc tcttagttaa gaaatctggg tttccaccta
    17641 aaactatatc tgtaccacgg ccagccatat ttgtagctat tgtaacagcg cctaatctac
    17701 cagcctgtgc tattatctct gcctctttat catgatgctt agcatttaat acttcatgtt
    17761 taatgccttt tttctttaat atttgtgata ataattctga tttttctatc gatactgtac
    17821 ctacaagtat tggctgatta actttatgtc tttcaattat ttcttgtgct acagcattaa
    17881 atttacctat ttcacttttg tatacagaat ctggtaaatc ttctcttacc ataagcttat
    17941 tggtaggtac ttggaaaacg tccattttat aaattgcttt aaactcttct tcttctgttt
    18001 ttgctgtacc agtcatacca gaaagttttt tatacattct aaagtagttt tggaatgtta
    18061 cagtagctag tgttttagat tctctttgta ttttaagacc ttctttagcc tctattgctt
    18121 gatgtaaacc ttctgaatat cttctaccaa acataagtct acctgtaaac tcatctacta
    18181 ttactatttc tccatcttta gcaacataat caacatcttt cttcattatt acatgtgctc
    18241 ttaaagcttg atttatatga tggtataatt ctgtatgaga tatatctgtt atattatcaa
    18301 cattaaaata cacttctgct ttttgtatac ccgatgctgt tagagataca gccttatctt
    18361 tttcttctaa ttcataatca tctggtttta aggttaaaac aaatgtgttt gcatctgaat
    18421 ataagtgagt tgatttgtct ccaggtccag atatgataag tggtgttcta gcctcatcta
    18481 ttagtataga gtcaacctca tccactatag cataatttaa tcctctttga actctttgct
    18541 ctttgtgtat taccatattg tcctttagat agtcaaatcc atattcatta tttgtaccat
    18601 aagttatatc acaatcatat tgttcttttc taacttttgg attttgaccg tgtataataa
    18661 cgcctacact cattcctaaa aactcataga tttttgccat ttggtctcta tcacgttttg
    18721 caaggtaatc atttactgtt acaacatgta caccttttcc agtaagcgca ttcaaataaa
    18781 caggagcagt tgcaaccaaa gttttacctt cacctgtttt catttctgct attcttcctt
    18841 gatgtagtac tatacctcca atcatttgaa ctctgtagtg tctaagtcct aacactctct
    18901 tagaaacttc tctaactact gcaaaagctt ctggaagtat atcatctata ctttcaccat
    18961 ttgctaatct ttctttaaat atatttgtca tatttttaag ttccgagtct gccatagatt
    19021 caaatttagg ttctaatgaa tctattatat caacagtttt attgaacttt tttaattctt
    19081 ttttatctgc catgttgaat aaattatcca taaagctcat attttacatc tctcctcgtt
    19141 ctttattgta ctattattca ttttaccata aaaactatac ttttaatatt aatttaaaaa
    19201 gctatcgata atttatttat cgatagctaa tatgattaaa tctaaattgc tttattcatg
    19261 ttctattata ccatatccac cattattacg cttataaact actgctattt catctgaatc
    19321 tatgttcttg aacatataaa aatcatgtcc tattaattcc atctgtaata cagcttcttc
    19381 ttcactcatt ggcttaacac taaatttctt acgtctttga attactaatt cttgattttc
    19441 atccccataa tcctcttgtt catcagctag atttatctct gaatcttgga atcttatact
    19501 cttgttgtct ttatgtttat cttgtaatct ctttttatat ttttttaatt gtttacttaa
    19561 tttatcatat acaacatcta tagctgtgta taaattttcc tgcatatctt ctgctctaat
    19621 tataggacca tttacagtag ctattgttac ttctattttt tgtcttgctt ttctcgcact
    19681 tactgtaact tttacatcag tgtctggatg aatgtaatta tctaacttac ttagtttagt
    19741 ttcgatttta ttttttattg catcagttaa ttcgatttgt tttccagata taattatgtt
    19801 cataaataca tctcccttca attatgtcag tataagaaaa tttatacttt aataatatat
    19861 tctacacgaa ctctcataat cctcttttta ttatttactt ttatgatttt ttctattcaa
    19921 ttgatagaat tatttatcat ttttagttac attgatactt atgtggatta atattattac
    19981 aaacacctgt ggataatgtg gataaaactg ttaataactc ttgtttttgt ttatttaaag
    20041 ctatttttac tcctatttct atccacaaaa atctccatta attgtttatt aatatgtgaa
    20101 ttcatgtcat aagatggtaa ttttaggaaa ttatgtgtat tatatgccaa tataccttta
    20161 taatttttta ataactataa accctctaaa acctccataa tctatctttt ctttcattat
    20221 ttatatttaa aactacttaa aagttataaa gcatactcaa caataatttc atatttaata
    20281 cctattaaca ttcatatacc caaattataa ttaacttact ctctttattt aaactaagta
    20341 ttttatattc atatactatc ttgaaattat atttacgagt tgtttaagct tttgtctatc
    20401 tatatcttca aaaggactgt tatttattct aatcgctgta ccaaaatgaa aattacttgc
    20461 tttagttaat tcctttatct tttctatatt attaaaatta agacctcctc ctaaaagaat
    20521 ttttatgtga ttagaactaa gcatcatatt ctttatcatt tgtatgttat caactatact
    20581 tcctttccct cctgatgtta atatattcgt aattttatca taatccttca aaattttaac
    20641 actatctatt gtatttgatt catctatggc tctatgaaat gtaacatcaa gattatcaca
    20701 acatttcaat aaaacattta gatttttctc atctatatta ttgtttttat ccaatacacc
    20761 aaatacaact ccattagcac caatctcttt aaccacacct atatcttttt gcattatact
    20821 tatatcttct ttactgtata caaaactttt cgcatgatgt cttatcatta cgttcacagg
    20881 tatctttaca ctgttaacaa cactttctat aagaccaaaa cttggtgtca atccaccttc
    20941 agttaaagca ctaactaact ctattctatc tgcaccacaa tcttctataa ttttagcgtc
    21001 ttctactgac attcctatta tttctaacat aactactcct ttgctaattc atatattgca
    21061 tttgcaaata tctttgcatt taatattata tcttctatat ccatgtattc atcaggatta
    21121 tgagctattc ctttttgtcc tggaaatgaa ggtccaaacg gaactatatt tggcattact
    21181 tttgcatacg tacctccatt agttgtcacg ggagttccat ctaatgatgt aaccctttca
    21241 taagctagct gaagcttttt aacaagatta ctctctttat caaaatatac aggattaaaa
    21301 ttagaaatta cctctatatc gatactattt ggaaaactag cttttatttc atctaataca
    21361 tctttcaatt tataactaaa atcgtatttt acctttatat ctaatatagt attactattt
    21421 tctatattca gctctttata tgtaacaagc acattttcag acatattttc tataaaacct
    21481 aggtatacat cttcttcata attgatttta ctttttattc taatctttgc catgcctttt
    21541 tcaccatata caacaggata tttacaatct ggtgtaaatc ccataatagg tgttttttct
    21601 ttttctaaat aatatggtat atcttcaaat ccactttctt catttgtacc aaatattatt
    21661 ctaactctct tatctaattt taagtttaat tctttaatag catataatcc atataaggca
    21721 gacattatag gtcctttatt gtctagtact cctcgtccat aaattcttcc attagtttct
    21781 tctcccttat aaggctgatg cttccaacca tctccttcat gtacaacatc tacatgtcca
    21841 attacacaaa cataatcatc actatctcca tgctctgcat aaccaatata attatccaag
    21901 tttctaacct taaatcccaa tttttttgat atttctaatg ctttattaag tgcctcccct
    21961 actttttcgc caaaagggaa gccattttct gactcccctt tgacactagg tatttttaca
    22021 atatctataa tatcacttaa taaatcttct tttaactcat ctattttatt acttatgata
    22081 ttcataaaaa tctccttatt ataaaactct attttaatta tgtaacacta ctttactcac
    22141 ttacttctat cggtgctttt attctagcct catatgcttc catccactca tttgatataa
    22201 ccttatgtat agtctttcca tctactctat ttgaaatata tacagcagga gattcttctt
    22261 tagttactac tgaaaaagtg aaatcaacta tatttgtagc tactccccat tcacctttat
    22321 tattcatggc aactaatgat aaatcaccag cttttcctct tcttttgata agctgtttat
    22381 caagttcatt tactgcaata tcacatgctt cttgtggatg ctttccttct ttcataagcc
    22441 taactatctc ataagatata catcccttca ttaaatcttc tcccaaacca gtagctgtag
    22501 ctcctcctat actactatca acatagaatc ctgaaccaga tagtgcagaa tctcctattc
    22561 tacctctttt cttcataaac aatccacttg ttgatgtagc tgcacacatt tttccagttt
    22621 tgtctaaaga tatcatacca acagtatcat gaccagcata tgggcttaat cctttgtcta
    22681 aagtctcttt ttttctcttt ttataatgta acttcgctct atcagtcaac atattttttc
    22741 tttcaaatcc atttttatga gcataatctt cagcacctat tccaactaaa aaattattta
    22801 ccttctcata acttaacttc ctagctatgc tgacaggatt agaataatct cttataccag
    22861 caactgcgcc aatatctaat gtgttaccat ccataaatgc agcgtcaagt tctacctcac
    22921 aattttcatt tggaagtcca ccatatccaa ccgatttata gaatggatag tcttcaactc
    22981 ttcttacagc aagctctaca gcatcacctg cttccatgcc tttttctaaa tgttctgatg
    23041 cctcagttat tccttctagg gccattctcc atgtcgctat cattccccac atgttaaaat
    23101 tcccctcctt aaattatata gattttacta atgtatgctt attataaata gtataatatt
    23161 taaacttgct aataattaat actcttatta ataacaaaat tcttctttca tcaaagttat
    23221 atcttcttta ttcgctattg cttttgccaa tttacatatg ttatttaatg aatcattaag
    23281 accaattcca ccttttttgg gagtcttaac tatacatatg tcatttttat attcgtttat
    23341 cacatctata ttttcttctg acatagctgc aaaagctggt atatctgttt tatcatttat
    23401 atttttagac agtattgctg acatcttaga aaagttttcg taattaaagc ttggtccaca
    23461 gattactaca tcagggttta atttttttac catagctatc atcttactcg ttacttcttt
    23521 ttcattattt acaaaaaatt catccccaca aaataaggta gctattacct tagattcatt
    23581 taaaaattgc tccatcatta cacctggtcc taatgggctt gattttccag caggaggtat
    23641 attacttttc tcttttcctc cagctccagc ttgtatttga tccaatatca taactatttt
    23701 cttcataatc ttcctcctat aatactaaat catcaaaaga tatatcatca tcttcctctt
    23761 caacagtttc attttcttct ctcaagtatt gcttatctag catttttata aatggtatat
    23821 acataaatat tcctagtata agaagcacta attgtaagac tgcaccttgc catcctgttg
    23881 ttaaaaaacc agagaatata attggagttg tccacggtaa ctgaactcca tttgtaagag
    23941 gcactaatcc tgctgacata cagaagtaag ctattattat atttattgtt ggaactattg
    24001 caaatggtat caatagtatt gggttaagca ttattggtag accaaatatc aatggctcat
    24061 ttataccaaa tattcctggt aaaattgata gttttcccat tgactttata cgtttacttt
    24121 tacatattaa taacatagct atgactaatg atagtgtact acctgctcca ccaaatgtcg
    24181 caaacatatc ttggaattga cctgttatta tgtttggtat ttttgaacca gcctgaaaag
    24241 cagctaagtt ttcagctgat agaattttta atattggatt atacacagcc ccaacaacac
    24301 tacttccatt tattccaaag aaccagaata aatgtaagaa tatataagcg attgccatag
    24361 cacctaaact atttcctaat tttaataatg gcatttgtaa aaacttaaat ataaattcat
    24421 gtatacttcc atatggtgta gcttcaaaag ctatcttaac taaaaagaaa actaccataa
    24481 ctattgcact tggtattaat gctgcaaatg attttgatac ggttggagga actccatctg
    24541 gcattttcaa tgtccatcct ttttttacaa ctgcagcaaa taattttaca gaggttattg
    24601 ctgttatcat acctacaaac attcccttag agcccatcca acctagtggt atacctgtta
    24661 ccatgtagac tgattctgtt ccttctggtg tatatggtat tacaaacggt gttactataa
    24721 aaaaggcaac tattgcaact gcagctgatg ctattggatc tacatctatt tgctttgcat
    24781 aagaatatcc aattcctaaa actgcaagta aagtcattac ttcaaatgtt gctacagttg
    24841 gttgttgcaa ttttacagcc caatctggac caaaaatttg acctacaaat tcattccatc
    24901 cttttatagg aaaatttgct actaataaaa ataatgaacc tattattaat aatggtgatg
    24961 atattaaaaa accatctcta attgcaatta ataatttatt tttacctatt ttttcagcca
    25021 atggcataag tattctttcc aatctattca tctatccacc ctcatttcct attatttttt
    25081 actataattc tacgcttgtt tagattttat tagctttata gcaagtttta atactttttc
    25141 cccattcata gttccataat caacttcatt gattatatct ataagttttc cttgagcttc
    25201 aaactttggt tttaactcct ttagcatgta tttaacttgt ggtcctaata atatacaatc
    25261 tggatttttt tcttcaacta ttttgtcaat ttctgctatc ggaaatgcct ctacttctat
    25321 tggtaaattg tgttcatctg ctaccttctg cattttatct gcaagtaaac ttgttgacat
    25381 accaaagcta caaaataaat atacttttct tttcattctt ttaaatcccc cttaattttt
    25441 ttattttaat ttttgaattt cttttcttaa cataatcatt tcttttataa ggtttctctc
    25501 actcatacaa gtcattaaat ggtcttgaga gtgaatcaat aaaatagata tatctgattt
    25561 atctccccca gcttcctttt gtataagttc agtttgtact ttatgtgctt ttaaaatttc
    25621 ttcatttgca ttttcaatta attcatctgc ttttttaaaa tcgttttcct ttgcatagtc
    25681 aagtgcttca taaagcatag attttgcatt tccagcatga attataatct ttaaaactat
    25741 ttcttccatt ttttcctcct aaattgcttc tctactttta gattatttat accttacaaa
    25801 catataatat cttataaaaa caaaaaaaca tgcacaactt tttccatcac atggaaaatt
    25861 ttgtgcttat ccatagttca ttatgaattt tatataagaa tatcttttac cttttcaatg
    25921 tcacatttac ttagaatatc tagcatattt tcatcctctg ttaaattaga tatttcttta
    25981 aataattctc taaatacttt tctatcatca tcttttttat atgctaataa aagtataatc
    26041 tttatatcct ttccatttct aatttttatt ggtgttttta accttgcaaa acataaaaca
    26101 gattgtttta tatatttcat gtctccatga gggaataata ttccttttcc aacattggtt
    26161 ggatatgact tttctcttgt tataatagat tgtaaatact ctttttctgc ataatgtttt
    26221 tcaattaata aattagttaa atatgttata gcttcataaa tatcatcatg ttctaactct
    26281 cttattaaat aatcagacat cattaatctg ttcataagtt tatatgaggt gtctttttct
    26341 tttattttgt ttgtaaactc tttaagttta ttctcatcct catcagtaaa taatggtgtt
    26401 gttataaaaa ttggtttttg gatattttca tactcttgaa atgcaattat aaaatctata
    26461 gtcttattaa agttatctct ttttaaatct tcctctctta atgtttcaat aatttttatg
    26521 ttatcaaatc tcttttctat ctttactttt aatagcatac ttactccaaa actaaatgga
    26581 catacaatac ttgccttttt atttgattcc cttttgctgt attttctttc tagagaggtt
    26641 tgaaagtgaa gtgtaagata accaatttca tcttccgtta tttgttttcc ttttaattca
    26701 atttctattg aacttgatat tactgtaaat aaaaaacaaa atttagtttt tatgttatct
    26761 aaaagtggat tttctaagta aacattgctt ttcatttgat gcattgtaac attcaaatga
    26821 catattaatt gttcataaag taatatatca ttattaaaat ttattcctga ttcttctgat
    26881 actaaattta taattttttt tgtataactt gctaattctt catttagata taaagatttc
    26941 ttaatattat tactatttat ttgtttgttc atacccaata ttaaagattc tataaacaat
    27001 ttttcttcat aatttaatac tatggataaa ttagatttca attcatcttc tatatccaat
    27061 ataagcattt ttagcttaag ctctttctta gtatttgata cttttaaaga atttcccagc
    27121 cttattcgta ttatatttat aagcaaaaat agcatcaatt gcttgaatga tatatctgta
    27181 agtcttatat ccatatcctc ctctatagta agcattatgt tttttacaat tattatatct
    27241 actatatgga agtactctat ttcatctatg tttaatctgt atttatctat gtatttaaat
    27301 aagaatgata ctaacattat tcttatgtct ttctcatccc cttcaatata tataccactg
    27361 ccttttttag tcgagacatt taaattataa ttatttaatg ctactgataa tttacttatt
    27421 tcttctctga caatattttt atttatgtac aacttattag ataaatcatt taaagtaact
    27481 tttttattat tacaaagtag taaatttaat atctcaactt gcttatgaag ctcactattt
    27541 actcttttaa cttcacctcg caacttctga tttaccagta ttttgtctgt tccatctcca
    27601 ctaaatttaa ttccaatatt aggttttctt ttgatataag cattacaaaa agtagataac
    27661 cattcatcaa tatatttgca atcattcctt attgtccttt ctgaacactc aaaatcattg
    27721 gcaatcttat taacatttat aaactcttct tttaatagca atgttgttaa taaattttct
    27781 tgtcttttat tcaaacatat cacctcttta tgactcaacc taaatttatt atatataaaa
    27841 aagagctata cataaaggta tgctagctct cttttaatat gatatttagc tgacctggtt
    27901 tttgacccca cactcgccta ctggagggtt cgcttgggtt cgccgcacta ctactctctc
    27961 tcgattttat tatcggtagg tcttacttct aagccgcacc atgatcatta acccaatttt
    28021 tagtgttaac ttacgtggat ccctttgcct gcgactggct tggactttca accacagacc
    28081 taaatatcac ttattcattt taatatttta aactaaaatt gtcaatatat aagattattc
    28141 cataatataa ttatcatttg cttttgtaag tagagtcaat acaaaaacct tattagcacc
    28201 tgataacttt aaaagtttag agatttcgtt tgttgttaga ccagtagtaa atatgtcatc
    28261 tattaaaagt atatttttat tatttattaa tttaacattt tctttaacaa caaatacatt
    28321 ttttaattct tcttttcgtt ctttcttatt caatttatat agcattcttg tatatttttt
    28381 tctacttatg caatctaata atggaatatt tactatttta cttaaattaa atgctatttt
    28441 ttgtgcctga ttaaaacctc ttttatttag ccttttttta tgaagtggaa caaataatat
    28501 ataatcaaat tttatgtttt ctaaatataa tttttctttc attatttgag ctatatactt
    28561 agccataaag gttttttggt tatacttcag ccctaacacc atcttttttg tcacatcatt
    28621 atattcaata caagatattg atttatcaaa ataaaagtct ttattaaaac aataactaca
    28681 ctcttcaata aattcttttt caattgaatg acgtattatt ggttttccac acttcatgca
    28741 accatcttgt ataaaattca tttctttaaa acaactttta catattgaat aagtatttgt
    28801 tttctttatg gatttatcac aaattatgca acttatattc tcaggatata taaaatccaa
    28861 acatttatta atcatatttt taaaattctt aataaaatta tcattgcata ttttcatatt
    28921 tattctatca acaacccttc ttgtttaaat ttatttagct tatatcctaa atttgaatac
    28981 ctttgattaa ctctattatt ttttatcata tattcaagat atttcacatc tccaactaat
    29041 acaactaatt tttttgctct tgttactgct gtatataata aattacggct aagtaacata
    29101 ggaggagccc acgctattgg caatactaca actggaaatt cacttccctg gcttttatgt
    29161 attgttgtac aaaaactatg gtcaatttca tctaattcat catataggta tgatacaatt
    29221 ttagtctggt caaataatat ataaatagtt ttcttatctt tatctatgtg ataaacatat
    29281 cctatatctc cattatagat accttctcca ctttcttttt ggtcttctgt ttcccatttt
    29341 tttgtataat tattttttac ttgcattacc ttatcgccaa ctctaaataa tcttttagaa
    29401 aaactttctt caactttaaa tttttctttt ttgttaagat acttctgtaa ctctatattg
    29461 agattagtaa ctcccaattc accttttctc attgaagata aaacttgtat atcttttaat
    29521 ttatcaactt tataaaattt aggtaatctt tcattaacca atcctattat ttcatttaaa
    29581 atttcttcat tagttgattt tcttataaaa aagaaatctt taccttttgt atttagataa
    29641 agtggttctc cattgtttat cttatgggca tttacaatta tcatactttc ttgcgcttgt
    29701 ctaaatattt catttaattt aactacattt ataatattag aattaatcat atccttaagt
    29761 acatttccag cacctacaga aggtaattgg tcactatctc caactaaaat tactcttgtt
    29821 cctaacttta tagctctaag taaattgtac attaagataa tgtctaccat tgatacctca
    29881 tcaacaatta ttacatctga atttattggg tcttcttcat ccttaaaaaa agttaaatca
    29941 tcatctgttg aaaatcccat ttctagcaac ctatgtatag tttttgcttc tttattagaa
    30001 gtttcactca ttctctttgc tgctcttccc gttggagctg ccaaagtcac acttttacca
    30061 ttattttcaa atatatctat gatagcatta atggtagttg ttttaccagt tcctggtcca
    30121 ccagttataa ttaatactcc actattaaca gattctttga ctgctaaaat ttgtttttct
    30181 gctaacttaa ttttcttatc ttcttctaaa acattaatct cgttatctat atctattttt
    30241 aagtcttcaa attcatactg tgataatttt actatttgac tacatactcc attttcagct
    30301 aaatagtatg gcataaggta tataagattt tcattgttta ctttctctat atgtattttt
    30361 tgattataaa ctagcatcat tacacactct tttattattt ctccatttag ctccaataat
    30421 ttttccgaat cttgtattag aacatgctct ggtaaatatg tatgcccatt acttaaagat
    30481 ttatttaaag tgtataagat tccctgacaa attctatctt tagagttttt atctatacca
    30541 attttacttg ctatactatc agctacttta aatccaattc ctcttatatc ttcagcaagt
    30601 tggtatgggt ttttatttat aacttctatt gcactgcttt tatatttttt atatattttt
    30661 aggcagtaat ttggtgttat tccaaaagga gatagctgta ttattatatt tctcaattct
    30721 ctgtcttctt cataactttt tactatttgt tttacttttt tactccctat accttctact
    30781 tcttgtaatt tttctggtga attttgtatc acctctaacg tatctacacc aaacttatca
    30841 attattcttt ttgccatttt ttcaccaata ccatgtatca ttccagaaga taagtaaaca
    30901 taaattcctt ctaaggatga tggcgttact ggcataaaac tattcacttc aaattgagtc
    30961 ccataaatct tatgatttac ccactttcct tcaacttcta tgctttctcc aattgccaaa
    31021 gtaggcatac atcctacaac cacaatttca tcattttcat ttaccaggtg agctattgta
    31081 taaccattat cttcgttttt aaagactatt tcacttatca ttccttctaa tttttccatt
    31141 tttatctcct aaaaatacta ataatcgctc catttaaatg ttagtataat tttatatatt
    31201 aaaactatga taatcgtaga aaagttttat atttttatac ttaacgtaat ttccaactat
    31261 tatacatttt atcagaaaca aaaaaaatct gcatccataa aatatggtac catgaaatac
    31321 aggtaccata tttaaatagc tattttattt taattactct atatttagag tataaattaa
    31381 attccataag tatattacag tactaactta tttcagattt attctccttg tacccctcag
    31441 ttttagtaat tcttctataa agcatacata gtaaaattaa aattcccatg taacctgtat
    31501 atggatatag tgtacctact aacttatcaa atggcagtaa acctcctata caagctaata
    31561 tacttaccac tattgttata attttaaatt tagggtggtc atcttctaca aatcggttag
    31621 ttactgacca taacaatggt acagctgtag tgtatattcc tagtaaaagt actactgaga
    31681 ataaaatacc tattattgga gatatcttat cagctaagta aagtgctggt atctcttttg
    31741 tgtatatatt tcctatgttt gaaagtaatg ccaaattcat cattatagct gctgccataa
    31801 gtgctactcc acctactata ccaccccata cagcatcttt cttatttact gcagaagctc
    31861 ccattcctgt caagaatgtt attactatta ttacattgta acaagtataa agtactcccg
    31921 agaaatatcc atttgatgta gcagttttaa tatttaaact atcaaccata tttccagcat
    31981 ttgataaagc atcaatattg ctaaataaac taattgctcc tactaaaagt gtaaatatta
    32041 ttattatagg tcctatatta cctagtattt tagaaagcct tgtcaaacct aatgaaacag
    32101 ttattaggga aacgattgcc ataccaatcc tacctacata tggatttaat ccatagtact
    32161 ctgatagtgt agctcctgct cctgcaatca ttattactaa tgttccaaat aaaaacaatg
    32221 gaccaaacca ttcaaaaaat gttccaagat attttccaca atatacttga taaattttta
    32281 ttggctcctt aagtttcaat tctcttccct tatctataac ttctgctcca aaccatgaaa
    32341 atagtaccat agagattatt ccacctataa tacccgcata tccataaaac gtaaaaaatt
    32401 gcagtatttc ttgaccagtt gcaaatccag aacctattac agtagcaaca taagctccag
    32461 caaaacttat aaccgtttta atatttactt tgcctgacat acactactcc cccccaatta
    32521 aaattatttt taaagtgtaa gtttattaag attatattag actcattatt gtgatatttc
    32581 aatatatttt taattattta acattagtta acacattctt aaccatcctt taacatatat
    32641 cccataatat gtataaattt agtagagtat tatataaaat gtattgacca gattaaatac
    32701 tgtttattca ttatatgatg ataaattaac atttattaaa aatatttaac tcataataaa
    32761 aaaactgtta gaaagtaatc gtaaaattac attctaacag ctttttagtt aaaaattatt
    32821 cacctaaagc atcttttctt aactgctcaa ccttgtcagt tctttcccaa ggtaaatcta
    32881 tatcagttct accaaagtga ccataagcag caaccttttt ataaagaggc tttcttaaat
    32941 ctaagtctct tattatcgct cccggtctta aatcaaagtg tttatttact aattcaacaa
    33001 gtttttcttc tgaaaccttg cctgtcccaa aagtatctat aaatatagat aatggtctag
    33061 ctatacctat agcatatgca agttctatct cacatttgtc tgcaagacca gccgcaacaa
    33121 tattttttgc aacatatcta gctgcataag cagcagatct atcaactttt gtagggtctt
    33181 ttcctgagaa agctcctcca ccatgtctag aatatccacc ataagtatct attattattt
    33241 ttcttcctgt aagacctgta tctccttgtg gtcctcctat aacgaatctt cctgttggat
    33301 ttatataaat tttagtctct tcatcaagta attctgctgg tataacttcc ttaataacat
    33361 gattaattaa atcttctctt attttatcat ttgatactgt ttcacaatgt tgagctgata
    33421 taagaactgt atgtactctt acagctttac ttccttcata ttcaacagta acttgagttt
    33481 ttccatctgg tcttaaataa tcaactaaac ctgtttttct aacttctgta agccttcttg
    33541 ataatttatg tgccaaagat atcggaagtg gcattaattc tggagtctca ttacatgcaa
    33601 atccaaacat tataccttgg tctccagctc caactttttc tatttcttct tctatctctt
    33661 ctccagtctt actttctaga ccttcatcaa ctcccatagc tatatctcca gattgttcat
    33721 caatagaagt tataactgcg caagtatcac aatcaaatcc atattttgct cttgtatatc
    33781 caatttctct tactgtttct cttactaatt taggaatgtc tacataagca gatgtactta
    33841 tttctcctgc tactaaaact aaaccagtag taactgtagt ttcacaagct actctagatt
    33901 gtgggtcttt ttctaataat gcatctaata ttgaatctga tatttggtca catatcttat
    33961 caggatgtcc ttctgtaact gattctgacg taaataaatg tcttgccatt tttattcccc
    34021 cttatttatt cttaattttt attataggtt ttaccttatt tttaatttta gaaatttact
    34081 caaaataaaa aaacctctcc tttataagaa gaggttaata atcgtaatta catactaatt
    34141 aactgacctc atctttcaag acttttgtct tgtaggaatt agcacctttt cccatttggg
    34201 tggttgctgg gcttcacagg gcctatccct cagcctctct tgataaggta atcaagattt
    34261 aagttttgtt ttactttact agcttactat ttttttaaaa ttttgtcaat atttttagtc
    34321 atattttttc tattcctttt tttgtaagac aaataaaatt ttctatctat ctgaaattta
    34381 aagttaggtc tttttaatgc tttttattca tatatttttt tgaagggggt gttcctattg
    34441 tatttagcta aagtaaacta cagcgatgat gatgttatac agatgctaag ttcagttcta
    34501 aaaactataa ttaatgaaaa tacgattgtt gtttgcatag gaacagacag ggctataggt
    34561 gatactttag gccctttagt tggaacaata cttaagaaca gtaactttaa ataccccgtt
    34621 tacggtacac tagataaccc tattcacgcc ttaaatatat atgagtcttt agatactata
    34681 aaaaatacac atatacaagg taacttcttg gctatagatg cttgcttggg gtcgcagagt
    34741 aacataggta atatacagat tagagaagga cctatcctgc ccggaaaagg tgttggtaaa
    34801 aaactacctc aaataggaaa ttattctata gtaggaattg tagataaaat tgatgaaaat
    34861 aataaacttt catttaataa tatacgcctt agctttatat tagatttagc tgaaacaata
    34921 gccttggctt tattagtatc cacctaatct aaatataaac taaatgataa aattctttat
    34981 ataaaaaggt agatatttta gagtatctga ttaaatactc caaattatct acctttttac
    35041 ttttatattt caagttttta tttttctact aaagcaaaca ttaattcacc tttacagaca
    35101 agttcttcac ctacatatgc cttagcatct gccttagcta tattttttct aagagttgtc
    35161 atttcaattt ccatagttaa tgtatctcct ggtcttactt ctctcttgat tctaacttta
    35221 tctatacctg caaatacacc tagttttcct ttgttttcct ctacagacat cattgccact
    35281 ccacatactt gtgctagagc ttctactatt agtactcctg gcatcaaagg atattctgga
    35341 aagtgacctt ggaaaaatgg ttcattaact gttacattct ttataccaac agctcttttc
    35401 ccaacttcta gctctgtaat cttgtctact aataaaaatg gatatctatg tggtattaat
    35461 ttttttattt catctatatt taacattcta aactctccta attattttct tttgaaagac
    35521 ccaccagttt caagtaagtc tattgaacct aaagctattc ctgtaccttt tgcaacacaa
    35581 gataatggtt catctgctat tgtaacagtt attcctgtcc tctgttctat tattttatct
    35641 aatccatata ataatgaacc tcctccagtc attattatac cgacatctcc tatatctgct
    35701 gctaactcag gaggcgtttt ttccaaaaca gcatgagtag caactactat ttgctcaaca
    35761 cattctctaa gtgcttccaa catttctgtt gaatttatag ttatagaact aggaagtcct
    35821 gtaattaaat ttcttcctgt tatcttcata tatttttctt cttctctctt aaatgcagaa
    35881 cctatttcaa acttgatttt ctctgctgtt ctttctccta taagaagatt gtgttgtttt
    35941 ttcatatatt taactatagc atcatcaaat ctatctccac caacctttat agattcactt
    36001 acaacagctc ctcctagaga tattacagct atatctacag tacctccacc aatatctata
    36061 atcatattac cattaggctg tgatatgtct acaccagctc ctatggcagc tgctattggt
    36121 tcttctatga tatatacttc tctagctcca gcttgtctcg tagcatcttc tactgctctt
    36181 ttttccactt ctgtgacacc tgttggtaca cataccataa ttcttggcat aaaaaatctt
    36241 ccaaatccct ttttatcaac tatcttatct ataaaagatt ttaacatctt ctcagttaca
    36301 tcataatctg aaattacacc atctcttaaa ggtcttatag cgactatatt acctggtgtt
    36361 ctccctatca tctttttagc ttcttctccc acagctaaaa cagaatctgt tcttttgtct
    36421 atcgccacaa cagatggttc ttctaaaacg attcccttac cactaacata aacaagcaca
    36481 tttgcagtac ctaaatctat gcctatatca gctccagcca ttttttcaac tcctttattt
    36541 ataatattag attatattat aagcctattt taatactcct ataataaagc atatacaaaa
    36601 atctcgcttt aatcaagaga aaataaatct tttgagagta ttattatttt gctatttata
    36661 attacttaat atatttcttt ttataattta attcaataaa aaaagtaggt ttttaaccta
    36721 cattttttta tgttccttct catattttaa tttcgttgct aatccccctc taatatgtct
    36781 ctctgattta tttttgtcca gcacattttt aacttctttg gcaagggatg gatttatttc
    36841 cttcaatctt tctgtaacat ccttatgaat tgtactttta cttactccaa atgtcttggc
    36901 ggtttgtctt actgtagtgt ttttttcaag tatatatttt gctaccacta tggccctttc
    36961 ctctatatga gatctcaaag attacccccc tcacataata gctttaatga tatatatgta
    37021 aaaacaccaa aaaatataac aagcttaacc aaatatgatt aaacttgtta tacttttact
    37081 taattagact cattgggtct atagattttt ctccactata cgcctctaaa tgaacatgta
    37141 taccttcttc actttctatt tgagaagtac tacctacagt acctagagat tgtccttctg
    37201 ttaccttctg ttctttctta acactaacat ttttatctag atttgaatat actactacaa
    37261 tgttattgtc actttttatt ttgactgatt gcccatattc ttcatcatta aatacatcta
    37321 ctactattcc gtttaataga gattttactt ctttgccttt agtacaacta atatctacac
    37381 ctttatgagt ttcccaaaca tcaagagttt tagaataact aggttctttt tctgaatatc
    37441 ctcttattac tttatctcca atgtaattta attttgttgt actcgattta ttttcctctt
    37501 ttgacttagc tttttctaag ttttgttttg aatccgttgc agtagggata gcatctttct
    37561 tgtctttttc aattaaatgt atttcctctt cactatcttt attagcattt tccataattc
    37621 ctttattaga agccaattta tctacattat tatttgtaaa ccaaactcca cctactgcta
    37681 ataaacaaac acatacaaac aaagataaat aaaaaccatc tttttctaac agctttttct
    37741 tcattttgca cctccaaata tatttctaca tttggattat tgacgtgttt ttggcatttt
    37801 atacaattat tagtatatat cttttatttt tgtatctgtg tagtaatgtg ataatatatc
    37861 ataatattta tagccctctt ctgccattcc ttctgctccc cactggctca taccaacacc
    37921 atgaccatat ccttttacca cgaaatctat gtatccttct ccaaatttta tgtcaaagtt
    37981 tgcagagttc aatttaaaaa cagttctaat gtcttttcca gtcaattctt tatttcctaa
    38041 ttttattttt tctacagttc cggcatcact tcttttagtt attgaaactt gtttacttaa
    38101 attattcaca tctatcacaa tagtgctata tgcccttcta agacttttaa caaaatctgt
    38161 gttacttatt ttaagtgttg acgcaaattt aggggaatac ttatcataag gactttccac
    38221 agactttaaa taaggatatt ttgcagaaaa tacttcttca ctattttctg tttttcctga
    38281 agatgtagaa aaataaagag gaagtatcgc cttatcatta taagttatta tttgaccttt
    38341 agttcctcta actgcttctt ggattttgga atatttattc ttaatccatt cttcaccatt
    38401 taatttttta agagtatcat aactcttata ttcttgacaa tgtttataat cagtacatac
    38461 tactgcattt ttatgtttac tagatttacc atgttcctgt ttatatacta catatgttct
    38521 agctgctaca gattgagctt ttaaagcttc tatatcaaac tctgaagaca tctcaccagc
    38581 tagtactcca cataaatagt tctccatgtc catcttttcg gttttccctg ttatatgatt
    38641 atatacattg ataattggtg cttttttatt tacagtttca tagctcactg tacttttttc
    38701 ttctttatta cctttgtctt caatatcact ttttttaata gttttgttta tagatactgg
    38761 gctctctggt ttttcagtta attctacatt tttataagaa actaaagtta taagtgatgg
    38821 aactaacaca gagcaagtca caaatcctaa caaaacaacc aatgggttct tcatatactc
    38881 tcctccttag cttttaaaac cttgtattta agtatatgaa caaccctatt aatttataac
    38941 tttcttctat aaaccaatta ttaatcttct actatttcga tttgaccacc tagtgctgtt
    39001 atcttcttat ctatatcaac ataacctctt tggatatggt atatttctcc tatttgagtt
    39061 tctccttctg ctataagacc gcataaaata agggctgccc ccgctcttaa atcagtagcg
    39121 tttactgctg caccgtgtag ctcatctaca ccattaacaa ccgcacttct tccatctatt
    39181 tttatattag cacccattct attaaattca gcaacatgca taaatctatt ttcaaaaaca
    39241 gtttctataa ctacaccagt tccattggct acagtaagca tagccataaa ttgtgcttga
    39301 acatctgtag gaaatcctgg atgtggtaaa gtttttatat ctattggttt taatactttt
    39361 ggacctacaa ctcttacaga attgtccatt tctgttattt cgcaaccagc ttctcttaat
    39421 ttagctacaa ctggttttaa atgctccatt aaaacatttt caacagttat atcacctttt
    39481 gtcatagctg ctgcaaccat ataagttgct gcttctattc tgtctggaat tacattatgt
    39541 tctgctcctt ttaactcttt aacacctttt atttttatag tattagtacc tgcaccttta
    39601 acatctgctc ccatttcgtt caagaagttt gctaaatcta ctatttctgg ctcttctgct
    39661 gcattctcta ttattgtagt cccttctgct aaagaagctg ccatcattat gttttcagtt
    39721 gctccaacag atgggaaatc taaatataat ttatttccta ctaacttttc tgttgctgct
    39781 tcaacaaaac catggtccat ttctatcttt gcacctaagg ctttaaatcc ttttaaatgt
    39841 aaatctatag gtcttgttcc tatagcacat cctcctggca tagaaatttt cgtactatta
    39901 aatctagcaa gcaatggtcc cataaccaaa aaagaagctc tcatctttct tacaagctca
    39961 tatggagctt cacaagtttt tatattgctt gcatcaacag taagtgtatt ttctttatat
    40021 tcaacttctg ctcctacatg tcttaataaa tctgatataa catgaacatc tcttaaattt
    40081 ggtactccat ttaatgttga ttttccgttt gctaataaag tcgctgctat tattggtaaa
    40141 actgcatttt ttgcaccatc tattttaaca ctacctttaa gtggattact cttttttact
    40201 attatcttag ccatattttt tgatctcctc ataattaata atctaatttt ataattggtg
    40261 tggcaatata tatgagtgtt tttccgtcat cctcattata tttaattcct atatttaaat
    40321 taattttttc ccctaaatat tctaattcat aatcatttaa tactttacta tatgctgtta
    40381 tagataaaaa gtttacgtct ttaacttcat ctatttgctt agcactcata ttatataata
    40441 ttttttgtaa aatatcatca tatttgttgt tttgtaacct ttttgtgtat tctccaacaa
    40501 tacaagcaga cacatctact tgagatgaat atttatttaa cacattttct acgttactat
    40561 aaataacctc tttatgttta tatactttgt tatccaatat gtcaactact atgtaacttt
    40621 ctttgttatt tttatttatt ccttttatag aaatttcttt atcgctacct tttatagtag
    40681 cacataattg tattttatcg ccagaccctt tttgattcca atttattcct tttttatcca
    40741 actttagatt gttaattatt tctataaaaa tattatgaaa ttctttattt gataaattat
    40801 gatttataac agaattaatt ttaacattat aaaatttaaa atttgcttct gatatttcaa
    40861 gagctttact taatctattg tatctagtat ttatacttgt atctgcataa gatattgtca
    40921 ttcctataat cattaatata aaaaagctta tagctaattt tacttttttc atagtaaaaa
    40981 atccccctta aatatgtttt tctgtataat agaattatta acaaatttaa gagagatata
    41041 ctttctcaat atgtattttt atacattttt gtatttttca tctaataatc tctttcaaac
    41101 tcttcaatct caattttctc tatccttctc ctggacttgc caattcctta tatgtatcaa
    41161 atacatcttg cattaattct tatatattat cttctgtaat ataaattact tagtctatac
    41221 ttaaatctat aatctataat ctataatcta taatctataa tctataatct attcatattt
    41281 taccagcaaa ataaacagat aatacttttt tatcaatttc catagctcca aatgaacata
    41341 cctaagtaca cttcccaata cacaatttac acactcttcc taataaatca aaaacacctt
    41401 tgttttaaac aactctattg tactttcaaa aactttattt gtgttctccc acttcaatta
    41461 aatttgaatt atcaagactc cagcccatcc atccatcact ataaagggag gtattttcca
    41521 ctcctatgac atttgcatat gttaatactt cagcagcacg ccatccactt ccacacataa
    41581 atataagctg tttattcaca tctatatttt ccttgtccca catttctaaa atctcagatt
    41641 tatttctcat tgtattatca atattacgat attcttctag tgaaacacta ttactacctg
    41701 aatgtccata taaagctcct ggtatacgcc cttttttatc ataatatgta tatccagata
    41761 cttttcctat atgttcatcc caagtacgat tatctactag tataaactta tttttttctt
    41821 ttagtttttg tcttaattca gatgtagtaa caactaattg tgagttaact ggtatatctg
    41881 ctccaaaatt agtacatgaa tgttttggat tactaataaa ttctaattca tatcctgctg
    41941 ataaccatga gtttgttcca ccattcaaaa ctcttacatc ttttactcct atatatctca
    42001 atataacagc tagtctataa gatgccatag gtgtaatact tgaaactatt acagtatcat
    42061 cctttgttag tccatagtct aaagcaaact tagttaagtt atcatcatta tcaagcatcc
    42121 atgttggagg aggttctata atatcagtat ttacatgaac acttgtaggt atatgccctt
    42181 ttgtatacga ttcttctccc caactcgctt caatcatttt tatattcttt gaatcttcaa
    42241 atgtctcagg aatttttcca tctaatatat cttttattat aaaagcagga actatcattt
    42301 gataattttt atatctttcc attggaagat tttcatcgtc tgcccattgt ttaatatcat
    42361 atttatatag atatttatat ccttttttag ataaataatc tgctacaacc aaagcatctt
    42421 ttccatttgc atcatacaat actatatttt tatctaaatc aattctttta gttttcaatg
    42481 cttgttctag cacttcatct tttctatctg agtatacatt taaccaatta gcagaaaaat
    42541 ctactgctct ttttatatgc ccacctcttt ttactccatc taacttccag ccattataag
    42601 catcatttaa gcgagtatca actataaccc aagcgttatc ttgcattttt tcttgtaaaa
    42661 cttcagtaga tattgcctta agattaacaa aataatcttc tttcttttct tttttaaaat
    42721 catttgtcat aatttatact cccaatttaa aaaaattttt atttgaaatt attttactaa
    42781 aataaagtaa ttttcttata ctctattagc ttcattatat tcttttataa atagttcctt
    42841 atattcttat tttaaactac tcactttatc ttatcacaag gttactaata ccttgtattt
    42901 taatttttct cataagctat tattttttta tgtctgatat tgactatatg tttattaagt
    42961 actcactata ttttatatga actatattta tcgttcccta tacctggtat ggtctatata
    43021 tttatcattt tacagatgat ttaatatcaa tctttttata aaatttatta tagcaaaaac
    43081 aaaacaaatt taatatttat ctaaaaaact catcctataa aatacaaata acaggatgag
    43141 tttttttcaa taaatattat ctatttaatt tttaatctgt ttttattcaa ctgttactga
    43201 tttagctaga tttcttggct tatctacatc acatcctctt agtacagaga catgatacgc
    43261 aagtaattgc attggtacaa ctgctaatat tggtgataaa atatcatctg aatttggtat
    43321 atagattatt ttatcagcag ctttctcaac atccttatta tggctttgag ctattgctac
    43381 tacataagct cctcttgctc ttacttcttc catattagaa accatttttt caaataactt
    43441 ttcttgagta gctatggcaa ttacaggagt tcctttttct attaaagcta ttgttccatg
    43501 ctttagctct cctgctgcaa aagcttctgc atgtatatat gaaatctctt ttaatttcaa
    43561 tgaaccttcc attgctaagc tatagtctat acctcttcct aaatagaatg catgttcaga
    43621 actaacaact gttttagcaa cttcttttat atattcttca ttgtctaata tttcttgtat
    43681 ttttgatggt atttctttca tcttagaaat catactatca taaaattctc tagttattgt
    43741 tccttttttg atagcaaagt ctaaagctat catgtataaa gaagttattt gagtagtata
    43801 tgccttagtt gatgcaactg ctacttcagg tcctgcccaa gtatagaata catcatctga
    43861 ttctcttgct atagatgaac ctacaacgtt agttattgaa agtattcttg ctcctttagc
    43921 ctttgaatct cttaatactg ctagagtatc tgctgtctca ccagattggc taactaatat
    43981 tactaatgaa ttttcatcta caaatgggtc gctatatcta aattctgatg ctatatcagt
    44041 tattactggt atatttacaa atttttctat ggcatactta ccaagtaatc cagcattata
    44101 tgctgttcca caagccacta tataaacttt atttattttt tctaagtctt ctttagaaat
    44161 atttatactg tctagaatta tatttccatt atcatcaagt cttctttcta aagtttctct
    44221 aactcctgtt ggttgctcat gaatttcttt tgacataaag tagtcatatc cacctttaga
    44281 cgcagcttct acatcccaag ttacatgaaa tacttctttt tcaacaagat ttctattaga
    44341 gtcataaact gttacatttt cgtcttttag atgaactact tctccatttt ctaagaaata
    44401 aacatctcta gtatatttta ataatgcagg tatatctgat gctataaagt tttcaccttc
    44461 accaacacca actacaagag gactatcttt tcttactgca actaattcat ttccatgttc
    44521 tttacatata acacctaatg cataagctcc tcttaatttt gatatagttt tgtatacagc
    44581 atctaataaa ttaccctcat agtatttatc tactaaatgt gctattacct ctgtatctgt
    44641 ttgagattca aacttaacac cttcagaaat taattcttct tttatttcca tatagttttc
    44701 tattatccca ttatgaacaa ctgctattgt tttggcttga ttaaaatgag gatgtgagtt
    44761 tacatcagat ggttctccat gagttgccca tctcgtatgc cctataccta aatttccatc
    44821 aataggattc ttttctaagt cttctgcaag tacagaaagt ctacctttaa actttctaat
    44881 attaagttct tcttcattag aactattaac tgctactcct gctgagtcat aacctctgta
    44941 ctctagctta gaaagccctt ctactataac ttctgctgcc tttctacttc ctaaatatcc
    45001 aactattcca cacattattt gtgtcctcct aaaaatatta tttatatatt ttaagagtta
    45061 ggtcatataa gtttactttg gcttcccttt gtccatagga ttgctcctat tatttaaaga
    45121 agtctttcga tggtaaacac accgcagagc atccgccgac aattcgatta actctgacct
    45181 cgtcaactta gttcctatat tactcaataa ttatgtctat aactaagttc tggcgcttag
    45241 aaaggtgtcc tcagtatttg tatctgagaa taccctttaa attacgataa tttttcttgt
    45301 ataagattag ctaagtttgt tgctaattct tttatttgac cttcctcttt accctctaac
    45361 ataactctaa ctaatggctc agttcctgat ggtcttatca aaactctacc atttccgttt
    45421 aaaatagatt ctattctttc tatctcagtt tttatttctg gatattccat atatttgttc
    45481 ttattttcat tttttattgt tgcatttact aatacttgtg ggtattggct cattattgat
    45541 gcaatttctg ataaaggttt cttttcttgc aagattatat ttgcaagtat tagagaacta
    45601 agcactccat ctccagtagt gttgtaatct aaaaatatca tatgtccaga ttgctctcca
    45661 ccaagattat atccattttt aaccatatct tctaaaacat atctgtctcc aactgctgta
    45721 gtagataaat ttattccatt ttccttagca gctattgtca accctatatt actcataact
    45781 gtaactacca aagtatcttg cgccaattta ttatttttct ttaaatttaa ggcactcaat
    45841 atcattatat ggtctccatc aacaatttga cccttttcat ttactgcaat taatctatca
    45901 gcatctccat catatgcaag acctaagtca gcattatgtt ccaaaacagc cctttgaagt
    45961 tgttctggat gtgtagagcc acatttatag tttatattat ttccatctgg tgaactattt
    46021 attgaaatta cacttgctcc tagttcatca aacactattg gtgccacttt atatgacgca
    46081 ccattagcac agtccaaaac tacttttaat cccttaaagt cagtgcttat tattgatttt
    46141 aggtagtcta catagtctct ttgtgcacaa tgttcatgta attttcttcc tacattttca
    46201 ccaattggaa gacaatcaat tttgtctata tcgtctatat attcttctat tttcaattca
    46261 atttcatcat ctaatttata tccattctta ttaaagaatt tgattccatt atattcaaca
    46321 ggattgtgag atgcagatat aaccacacca caatcagcac catattttct agttaaatat
    46381 gccactgcag gtgttggtat tattccaaca gtaataacat cacatccaac agacataagt
    46441 cctgctatta aagatgcttc caacatatca cctgatattc tagtatcctt acctactact
    46501 acttttactc tgtggtctcc ttgagctaag acaaatccac cagctctacc aagtttatat
    46561 gctaaatcac atgttagttc tgtatttgct actcctctaa ctccatctgt tccaaaatac
    46621 tttctcacta aagattctcc tttcataatt gaaaagcttg taatttgtat tatattttta
    46681 tagttcataa aatatcatta taggcttaat ttcagtcata aaaacatttt aatacttatc
    46741 agcaaaaaag acaatagaaa taattctatt gtcttttttt tgacatcttt ttctttatta
    46801 gttagttgct ttagaacatg tagcatccat acctttttgt aaagcttctc tgtttaatgg
    46861 aacgaatttt tcttttctag gtccgaaaac ttttttgaat gcttctagta cagattctat
    46921 atctacagta tcagatactt ttaagtaagc gcctaacatt atcatattag caactttatc
    46981 atttcctaat tcagctgcta attcatttgc tggtatataa taaacatcta catctgttct
    47041 atcaactttt tcttttatta aagaactatt tactattatt tttccaccag gaataacatc
    47101 atctttgaat ttttcaaatg ctggtatatt taataatata gctgcagttg catcatctgt
    47161 tataagtgga gaaccaactg gctcattaga tacagttact gcacagtttg ctgtacctcc
    47221 acgcatttct ggtccataag atggtaacca agaaacttct tttccttcta gcattcctgc
    47281 ataagtaagt aattgtccca tagacataac accttggcct ccgaagcctg cacatattac
    47341 tctagctgta gacatttact tcacctcctc aacttcaaca tttttgaaat ttcctaatgg
    47401 atagtatggt atcatgttgt ctcttaacca ttgtaaagca tcgtttggag ctagtcccca
    47461 gtttgtagga caagtagata atacttctac tattccaaat cctaatcctg cttgttgaac
    47521 ttggaatgct ttctttatag cttttttagc ttgtctaaca tgagctggag tatcaactgc
    47581 tactctttct acaaatacag ctcctgttaa tgtagctaac atttcactta ctcttattgg
    47641 ataaccttga gtttcagcat ttcttcctga ttgagcagta gtagctcttt gtccaactaa
    47701 tgtagttgga gccatttgtc caccagtcat accataagtt gtattgttta caaatatagt
    47761 agttattttt tctcctctag cagctgcatg aactatttca gcagtaccaa ttgaagctaa
    47821 gtctccatca ccttgataag tgaatactgt attttcagga tgaactcttt ttattcctgt
    47881 agcagcagct ggcgctctac catgagcagc ttcttgagtg tcacagttaa aatatttata
    47941 tcctaaaact gaacatccaa ctggaacaac acctacagca tcacctaata ctcctagttc
    48001 ttctaatact tcaccaacta atctatggat tataccatgt gtacatcctg gacagtagtg
    48061 agtttgagta tcttgtaatc cttcagtttt cttaaataca acagccatta tctagcacct
    48121 cctgcaacca attctccggc aataattttt ttagcttttt caactatttc tgctggtgtt
    48181 ggagtcattc ctcctggtct tccatggaag taaactggta atttaccttc tactgccatt
    48241 ttaacatctt ccaccatttg tcccatactc atttcaacag tcaatatgtt tctagcatta
    48301 ggaatttggt tgaatgcttc gaatgggaat ggccataata ctttaggtct tataagacct
    48361 gcttttattc cttcttctct taatatatca attgcgcttt ttactactct tgaagtagtt
    48421 ccataagctg caaatacaaa ttcagcatct tctgttttgt acatttcgta ttgtacttca
    48481 ttcttttcca ttgcatcaaa tttttcttgt aaatgccaac aatggtcttc tagtacttct
    48541 ggttctaagt atagagagtt tattacatta ggttttcttt ttcctttagt tccaacagtt
    48601 gcccaatctt taggaggcaa ttctcttttc ttctcaggag ctctaaattc aactggctcc
    48661 atcatttgac ctatcatacc atctgcaact accataactg gtgatctgta ataatctgca
    48721 acgtcaaaag cttccattat catatcaaca gcttcttgaa ctgtagctgg tgcgaatact
    48781 ggagttctgt agtctccatt tcctccacct cttgtagaca taaagtagtc agcttgtgaa
    48841 ggttgtatac ttccaagtcc tggaccacct ctcattacat taagtacaac acaaggaact
    48901 tctgctccta ctgcatatgt tataccttct tgctttaaag caactcctgg tgaagatgaa
    48961 gaagtcataa ctcttgctcc tgcacctgct ccaccataaa ccatgtttat cgcagaaact
    49021 tctgattcag cttgaacgaa cgctccacct attttaggta actctcttga taaatattct
    49081 ggtaattcac tttgtggagt tattgggtaa ccgaagaaat atttgcaacc agcttctata
    49141 gctgcttttc caaatgcttc gttacctttc ataagtatct tagccattaa aattccccct
    49201 taaatattaa ttaaattaat ctctttctac tgttataact acatctggac acatagttgc
    49261 acaacttgca catcctatgc atttttccat ttcaaaaaca gttgctgggt tatacccttt
    49321 tttgtttatt acatttgagt ctaattgtat gatttttact ggacatgctt caacacacaa
    49381 tccacaacct ttacaacgtt cttgattaaa agatactttt cctttagcca tttacctaag
    49441 cctccttaaa ctttctatat atttgagctc aaactacatc cagtcttctc tcatatataa
    49501 ttttatagga aaaatttctc cttctaaatc ttttggtaac tgttcaacaa gattttctaa
    49561 acatgcaaca tatcttatcg gtatattaca tttttcagaa acttcttttg caactttctg
    49621 acctcttaaa acatcatcaa tagtagtaaa cctaataaga tgagtattat taattagacc
    49681 tgttaccttt aatttagaag atttttctat ttcttttata tattggataa cttcttcaga
    49741 agtttgagtt ttctccctat ttgcatttat tacaaaaagc atatcataat cgccttcttt
    49801 taacaaatga ctaaatcttc ctataaccct agcacctaca ttatctccac ctaaatctat
    49861 aacagaattg tatgaatggt ctaccatagg agacattacc tctgctgata ctgctggtaa
    49921 atctaatgtt ggagcattaa ttgagctatc tattggttgt atacctaagg atttcattaa
    49981 ctctttcttt tctctagttc taaagtatac atttaccaca tctaaatcgg ctatagcaac
    50041 tttgccagaa accatatctc ttaacttaac aacatagttc atcgcaaatt cacttttacc
    50101 acttccatag tgaccagtta tgattcttat tcttttatca ttagttatca taaacttgag
    50161 cctcttcttc accagttaaa actctaagtc caccttgagc taatgcaatc atttcatctt
    50221 cacctggata aacttttaca tctgctataa atttaactct atctgcaatc atttctgtaa
    50281 acatttttga atatgcgatt ccaccagtta ataatattgc ttttacatct cccttaagaa
    50341 ctgcagcact agctcctatt tctttagaga tttgatatgc catagcttca tatactaatt
    50401 tagctttttc atcgccagct tcaattcttt cttcaacttc tctagcatca ttagtgttta
    50461 agtatgcaac taatccgcca ttacctttta ttttcttttt aatttcatct tgagtatatt
    50521 ttccactaaa gcacattttt actaatgcac ctactggtag tccaccactt ctttctggag
    50581 agaaaggtcc ttctccatct aatgcgtttg caacatctac tattttacca tttttatgag
    50641 ctccaacaga aactcctcca cccatgtgtg caactataag atttatatct tcatatttct
    50701 tgtttatttc tctagcatat cttcttgcta ttgccttttg atttaaagca tgtactacac
    50761 ttgctctact tatttcaggc ataccagaaa ttctagcaac atcttctaat tcatctacaa
    50821 caacagggtc tactatgtat gaaggaacat ttacttcttc acctatttgt tttgctatta
    50881 ttccacctag atttgaagcg tgttctccta aaactcccac ttttaaatct tcaatcatag
    50941 cagcacttac tgaataagta ccacctttta taggtttaag aagtcctcct ctacctacta
    51001 cagcatctaa ttcagatgtt tttactccac cttcttttag agcttcttct attacttgtt
    51061 tacgaaattc aaattggtca gacaccttct catattttcc tatttcttct gaagaatgtc
    51121 ttaaagtttt ttcaaatact aaatcctcat tatcaaatac agctatttta gttgatgtcg
    51181 aaccaggatt tattgttaag attttaaata ttttgctcat ttgcctattt cccccttaaa
    51241 attatgcctt tgctgccatt aaaacaccta aagctattga gtttagttta gtttcttcac
    51301 tgtctgctct agaagttaat attattggtg ctttagcccc aactataact cctgcatttt
    51361 ttgattttga gaagaatact aaagctttat ataatatgtt accaccttca atatctgggg
    51421 ctaataatat atcagctctt cctgctacag gatgatttat acctttatgt ttagctgctt
    51481 ctaaagatac tgcattatca attgcaaaag gtccaccaac catacaacct ttgatttctc
    51541 ctctttcata catttcttct agttctttag cttcaactgt atctttcatt tttggattta
    51601 ctttttcttt tgcgcatatt gcagcaactt ttggttcact tatatctaat gaatgtgcta
    51661 ctgtgcaagc attttctatg atttgctttt tagtatttgt atcaggagct aagttcatag
    51721 ctgcgtcagt tacgaaaaat aatctatcat atccctctac atcaaatact gctacgtgac
    51781 ttaatacatt tccagttcta agacctactt ctttatttaa aactgctttt agtattattg
    51841 atgtgtctac taagcctttc attaccatgt cggcttttcc ttgtgaaact aattcaacag
    51901 attttagaga tgcttctgct aaatctttta tatctatcag ttcataattt tcgatatcca
    51961 tgtctatgct ttttgcaatt tctttagtct tttctatatc tcctactaaa atggcatttg
    52021 ctattttttc ttttctagcc atttcaactg ccattaaaac ttctttatct tggcaacatg
    52081 ctactgatat agttttaggt cctctttctt ttgcaaactt aattacttct tcaaaacttc
    52141 tcatgcattt ccacctcatc ttgaattttt tgaaatagaa ctttttatat ctcaattaaa
    52201 ttataactcg ttcagcaacg ttttcctaga gataattgaa atatttaatt caaaatattt
    52261 atttaaatct atttatcagt cttttttcat atatctagcg ctttttagat ttaacaaata
    52321 tctcattgtc cttattttat aatataacta aaataaacaa gatgcaacaa aaacataaaa
    52381 ttaacaaact taattttata ggtttttttg tcgcatcttg taacaactaa atattcgaaa
    52441 tatttgttca ttccacttca acaatattag gttctatatt tattttttta aattgatatg
    52501 ttgtttcata ttttatttta tatttactat cttcctcagg gccttgtgat aaatcaatat
    52561 ataatgaaat atctttttta tttaacttac tcaaatcctc cgttgactct acaacaactt
    52621 ttatatctac atcagaaatt acatttggat tatctatatt tgttgggata tttcttaatt
    52681 caatatctcc cgcattatat aaaaattctg cactcaattg ttttgtatca tttaatttaa
    52741 ttgttataga gctatcctca agagttatac cctctggaat ttctaagcta acttcttttt
    52801 cttcaccatt agccaaatca gctaagtcaa taggttttgt cttaatataa cttattttgt
    52861 ctatatcttc tttctttcct tttattgtaa cttgttcctt acttaattta tagtttttca
    52921 gtacatcatt atctttatta tcatttagac ttactcttat tgggacaatt ttttccttca
    52981 atagttctat ctttgcagtc acaaaggatg aacttaatgt tactccttca accttagttc
    53041 catcactatt taatggtatt aattgcaatt ttttagaaaa atcatctgtc tcattaccaa
    53101 catctagatt agctcttaca cttttgactt ctttaactag agttcttgga cctgatacat
    53161 cgacaaattc cttactaagt tcaattttat ttatatttct ttttgaattt ccacttactt
    53221 ctacctttat atctcttttt tcttttatgt ttttttcaag atttactact aaaacatcat
    53281 gcttaaattc tagagtaaca ccttgaggaa tatttgcttt taaatatact tccttctgtc
    53341 cttgtattgg gtcttcaatg gttccatata catgtatatc ttctttctta acactttgta
    53401 cctttgataa actacctttt atatatacat ctgctgttaa tttttcgtct ggatatataa
    53461 caagattttt atcctttaaa tcactcatat tacttataga tacaggtata tcttcaaacc
    53521 ctttagtttc ttcagggtca actacagcca taacatacat ccataatgca attgcactta
    53581 aaaatgatat tatttttatt ttggtattat tttttagtct atttatcatt taaatatacc
    53641 acctttgaaa aaacttcttg tttctgtatt tgtttttaaa ttactacgta atatattcat
    53701 cattctttct ctagaaatat ctctatataa cttacctgct ttagcaattg atacgccacc
    53761 tgtttcctct gatacaatca gagttataca gtcagacacc tcacttaccc ctattcctgc
    53821 tctatgtcta gtacccaaat ctttacttaa atccttactt tccgtcaatg gcaaaaaaca
    53881 tgcagctgct tttaccttag aatctctaat tacaacagct ccatcgtgta atggtgtatt
    53941 tggtataaat atatttatta atagttgcct agaaatttca gcatcaataa tagttcctgt
    54001 atttattata tcacttattc tagtttctct ctccattatg ataagagctc ctattttttg
    54061 tctagaaagt gaatataaag cttctacaat ttcttctata tttctattga gagtttcttc
    54121 agaagtattt acattctttc caaataaatt aaactttgct cttcctatat gttcaagtcc
    54181 tgctctaaat tctggttgaa aaattataag agcagctata actccataat ctaaagcttt
    54241 aagagatatc caataaacgg tatgcaattt aaaggtgtta ctaagttgag ttgctaataa
    54301 aagaaatatt atacctttaa acacttgttc tgctcttgta tcttttataa acataaatat
    54361 tttgtaaaaa atatacgcta ctatagatat gtcaattaaa tcatatatac tcattcttaa
    54421 tattatatta aaaaatccat ttaaaaaaga atttatatct aacacagttt tatcctcctg
    54481 tttgataact tcttaattat ttacactaag tattatacta tataaatcta actctataca
    54541 aactatttaa cttttatgta aataattcta gttttatatt tggagattgt taaataatct
    54601 ctaaacacat taaaaatatc aacttaataa atacatattt cttattaaag ttagttttta
    54661 ctcaaataac tcaaaccatc aaggcgcgaa tacaaaatag tactcgcgcc ttaatgcagt
    54721 tttatgttct aaaatatctt aatatatctt tgtttaatat atattaatta ttataaattc
    54781 tataatccac ttattaacta taaacaaata caaaatctaa atattactta tcatcaatat
    54841 tattccatta gattcatttt tatattaaag taataactta tttcatatct agattagata
    54901 aatttatttt aatatcttac tataaactat cattaaaact tattatgtat aactagatat
    54961 aatctcctac agtgaaaata atacacaata cctaaagtta tatatccatt
//
LOCUS       DAELSY010000005        54974 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.005, whole genome shotgun sequence.
ACCESSION   DAELSY010000005 DAELSY010000000
VERSION     DAELSY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54974
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.005"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..417
                     /locus_tag="KPG09_000260"
     CDS             <1..417
                     /locus_tag="KPG09_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBG0964889.1"
                     /translation="YSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNV
                     GELIKLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLG
                     VFNKRPNGPCVNTNVDMMDIEKAIDFLTGKNILICN"
     gene            486..1187
                     /locus_tag="KPG09_000261"
     CDS             486..1187
                     /locus_tag="KPG09_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBG0964890.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            1249..2574
                     /gene="rsmB"
                     /locus_tag="KPG09_000262"
     CDS             1249..2574
                     /gene="rsmB"
                     /locus_tag="KPG09_000262"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBG0964891.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            2582..3613
                     /gene="rlmN"
                     /locus_tag="KPG09_000263"
     CDS             2582..3613
                     /gene="rlmN"
                     /locus_tag="KPG09_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBG0964892.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGNIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            3633..4385
                     /locus_tag="KPG09_000264"
     CDS             3633..4385
                     /locus_tag="KPG09_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBG0964893.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            4402..6417
                     /gene="pknB"
                     /locus_tag="KPG09_000265"
     CDS             4402..6417
                     /gene="pknB"
                     /locus_tag="KPG09_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBG0964894.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     EEEEEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGSSNSGNSGGNSGNNGGNSGGSNSGNSGGSSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            6525..7262
                     /locus_tag="KPG09_000266"
     CDS             6525..7262
                     /locus_tag="KPG09_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG0964895.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVENMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            7360..8244
                     /gene="rsgA"
                     /locus_tag="KPG09_000267"
     CDS             7360..8244
                     /gene="rsgA"
                     /locus_tag="KPG09_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG0964896.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            8249..8920
                     /locus_tag="KPG09_000268"
     CDS             8249..8920
                     /locus_tag="KPG09_000268"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG0964897.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            8929..9570
                     /locus_tag="KPG09_000269"
     CDS             8929..9570
                     /locus_tag="KPG09_000269"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBG0964898.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTVSVTAI
                     KGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            9701..10330
                     /locus_tag="KPG09_000270"
     CDS             9701..10330
                     /locus_tag="KPG09_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG0964899.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSKHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            10385..11041
                     /locus_tag="KPG09_000271"
     CDS             10385..11041
                     /locus_tag="KPG09_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0964900.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGASIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            11456..12172
                     /locus_tag="KPG09_000272"
     CDS             11456..12172
                     /locus_tag="KPG09_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0964901.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMRLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVNIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAINTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            12147..14480
                     /locus_tag="KPG09_000273"
     CDS             12147..14480
                     /locus_tag="KPG09_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0964902.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQAGAKGGA
                     KNPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKVINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLL
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(14797..17475)
                     /gene="mngB"
                     /locus_tag="KPG09_000274"
     CDS             complement(14797..17475)
                     /gene="mngB"
                     /locus_tag="KPG09_000274"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG0964903.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISNIENYIDSVKNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTANEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESSKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERSIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLLFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNNSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(17550..18656)
                     /locus_tag="KPG09_000275"
     CDS             complement(17550..18656)
                     /locus_tag="KPG09_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG0964904.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            complement(18770..19078)
                     /locus_tag="KPG09_000276"
     CDS             complement(18770..19078)
                     /locus_tag="KPG09_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG0964905.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            complement(19081..19533)
                     /locus_tag="KPG09_000277"
     CDS             complement(19081..19533)
                     /locus_tag="KPG09_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0964906.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGIATNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            19767..21695
                     /locus_tag="KPG09_000278"
     CDS             19767..21695
                     /locus_tag="KPG09_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0964907.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITISRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQNNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            complement(21955..22716)
                     /locus_tag="KPG09_000279"
     CDS             complement(21955..22716)
                     /locus_tag="KPG09_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0964908.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            22935..23411
                     /locus_tag="KPG09_000280"
     CDS             22935..23411
                     /locus_tag="KPG09_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG0964909.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            23509..23661
                     /locus_tag="KPG09_000281"
     CDS             23509..23661
                     /locus_tag="KPG09_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBG0964910.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(23821..24009)
                     /locus_tag="KPG09_000282"
     CDS             complement(23821..24009)
                     /locus_tag="KPG09_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBG0964911.1"
                     /translation="MAKVCSVCDKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            24370..24720
                     /locus_tag="KPG09_000283"
     CDS             24370..24720
                     /locus_tag="KPG09_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG0964912.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            24739..26382
                     /locus_tag="KPG09_000284"
     CDS             24739..26382
                     /locus_tag="KPG09_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBG0964913.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            26463..28520
                     /gene="recG"
                     /locus_tag="KPG09_000285"
     CDS             26463..28520
                     /gene="recG"
                     /locus_tag="KPG09_000285"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBG0964914.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIRNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMERFKNKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            28579..29133
                     /gene="rsmD"
                     /locus_tag="KPG09_000286"
     CDS             28579..29133
                     /gene="rsmD"
                     /locus_tag="KPG09_000286"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBG0964915.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNVKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            29135..29632
                     /gene="coaD"
                     /locus_tag="KPG09_000287"
     CDS             29135..29632
                     /gene="coaD"
                     /locus_tag="KPG09_000287"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBG0964916.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            29636..30160
                     /locus_tag="KPG09_000288"
     CDS             29636..30160
                     /locus_tag="KPG09_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964917.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            30504..32423
                     /locus_tag="KPG09_000289"
     CDS             30504..32423
                     /locus_tag="KPG09_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0964918.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQERFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISKEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            32436..32723
                     /locus_tag="KPG09_000290"
     CDS             32436..32723
                     /locus_tag="KPG09_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0964919.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            32856..34115
                     /locus_tag="KPG09_000291"
     CDS             32856..34115
                     /locus_tag="KPG09_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBG0964920.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISKFIGSLFGGIL"
     gene            34118..34858
                     /locus_tag="KPG09_000292"
     CDS             34118..34858
                     /locus_tag="KPG09_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0964921.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQILVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            35181..36491
                     /locus_tag="KPG09_000293"
     CDS             35181..36491
                     /locus_tag="KPG09_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG0964922.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            36589..37506
                     /locus_tag="KPG09_000294"
     CDS             36589..37506
                     /locus_tag="KPG09_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG0964923.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            37522..38331
                     /locus_tag="KPG09_000295"
     CDS             37522..38331
                     /locus_tag="KPG09_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG0964924.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            38388..40136
                     /locus_tag="KPG09_000296"
     CDS             38388..40136
                     /locus_tag="KPG09_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBG0964925.1"
                     /translation="MEISYVLKDCTFKHNKYSVIEAETWKEKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSTISWKGLENRVRLALNVPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLNIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDKSKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMSKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDENDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIENLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            40399..41421
                     /locus_tag="KPG09_000297"
     CDS             40399..41421
                     /locus_tag="KPG09_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBG0964926.1"
                     /translation="MFGGINIKRIGIIIMFFLSIAFIGFYLAIRGNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEEFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLSSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNVPKDSNIVV
                     SFHYYEPNDFAFQGNIYHNGFEHLSNITWEGTNEQMDYLKKRFDIVENWANKNKVKIF
                     LGEFGVTKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            41426..43732
                     /locus_tag="KPG09_000298"
     CDS             41426..43732
                     /locus_tag="KPG09_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0964927.1"
                     /translation="MKKNSKKILITLTIITNIVYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPVISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKNKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKIRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKNIYPKEIALEINENIVLECDLCEGKIDERGLLYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTIPVSNPSEKKEKSIDEFEPMEYI"
     gene            43704..44819
                     /locus_tag="KPG09_000299"
     CDS             43704..44819
                     /locus_tag="KPG09_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964928.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSAKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSRITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQKINLSIQSKNMLEFRLKEGSEIFLEDKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEKSNVVLDSSMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     SFLHNIKITGNEEVQIPVLGQSISQYEVLGLKRDSKIKYSLMGKQDNVSISQNGKLTL
                     SNKAEPGQIILQVNVDDKFKIGRKIALTESWSINKKDKDGVPYTLVSPEQSPTVQYMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            44837..46255
                     /locus_tag="KPG09_000300"
     CDS             44837..46255
                     /locus_tag="KPG09_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBG0964929.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCVYNIGVLFIFKYLAFAL
                     RNISELINTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTMQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMLGG
                     NGVIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            46270..47478
                     /locus_tag="KPG09_000301"
     CDS             46270..47478
                     /locus_tag="KPG09_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964930.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNICYSGKDMIQEIKNSVAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSEEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKENYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCK"
     gene            complement(47559..48947)
                     /locus_tag="KPG09_000302"
     CDS             complement(47559..48947)
                     /locus_tag="KPG09_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBG0964931.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFTIHLIFITLNGLNPITYIRNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSLYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFGKKNIS"
     gene            49289..50623
                     /locus_tag="KPG09_000303"
     CDS             49289..50623
                     /locus_tag="KPG09_000303"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBG0964932.1"
                     /translation="MRVVIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFDDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITEKQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(50752..51669)
                     /locus_tag="KPG09_000304"
     CDS             complement(50752..51669)
                     /locus_tag="KPG09_000304"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBG0964933.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLNSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            51877..52761
                     /locus_tag="KPG09_000305"
     CDS             51877..52761
                     /locus_tag="KPG09_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBG0964934.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRIKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGKKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            52974..54815
                     /locus_tag="KPG09_000306"
     CDS             52974..54815
                     /locus_tag="KPG09_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBG0964935.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKNKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDYVVSKKVDEIKEQQEKILDKVIGATTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGERITSIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPILKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNQGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
BASE COUNT        21225 a         6076 c         9457 g        18216 t
ORIGIN      
        1 tacagtaata aatataattt taatccagag gatataataa ttttaatacc tcattgtatt
       61 caagagaata gttgtaaatt aaaagttact aatgatatag ataattgtaa agaatgtgga
      121 agatgcaacg taggagaatt aattaagcta aaggaaaaaa ctaatgtaaa aatatttgta
      181 gctacaggag gaaccctagc tagaaaaatt ataatggata ataaacctaa agctgttgta
      241 gctgttgcat gtgaaagaga tttgacatct ggaatacaag atattaagaa aattcctgtt
      301 ttaggtgtat ttaacaaaag acctaatgga ccttgtgtaa atactaatgt agacatgatg
      361 gatattgaaa aagctataga ctttttaaca ggaaaaaata tattaatttg taattaaact
      421 gtttttaagt taagtgaata ttaagaggat gtataataaa aatatatttt aggagagtgt
      481 tatttatgta ccctgtatat ggcggttttt ggggatttga cccaacaatg gttgtattaa
      541 ttccagctat attacttact atatatgcac aatttaaagt gagttcaaca acaaataagt
      601 atttaagagt aaatacacgt cgaggatata ctggagagca aacagcaaga agagtacttg
      661 attcaaatgg tttgtatgat gtaaagatag aaatggttag agggcatcta agtgaccatt
      721 atgacccaag aagaaaggct gtaaggttgt cagaagatgt atattatgga acatctataa
      781 cttctgttgc agtagctgca catgagtgtg gtcatgccat acaacatgct aaaggttatg
      841 cgcctcttca aataagaagt agtttagtac cagtagtgaa ttttgcttca agtatatctt
      901 ggtttttaat atttttagga tttattatgg ctggtccatt tttaaaaatt ggtatactat
      961 tgttttcggc ttcagtgtta ttccaaataa taacattgcc tgtagaattt aatgcttcaa
     1021 gtagagcaat cgttcaactt gggaatttag gtattataga tgaaagtgag tcaagacaaa
     1081 gtagaagagt actttcagca gcagctttaa catatgttgc agcagcattg gtttcaatac
     1141 ttcaattact tagattgtta ctaatagctc aaagaagaaa tgactaaaag taaagtgggt
     1201 tgtttagaaa caaccctttt ttatgtttaa taaaagggag aaatttatat ggatgcaaga
     1261 gaagttggat ttaaagtatt atgtgatata gaaaaaaatg acaattattc aaatattgca
     1321 ataaataagc attttaaaaa tttagaaata agtgatatgg atagaggact tgcaacagag
     1381 ttaatttatg gagtaataga aaataaatat tatttagatt atataatcaa taaactttca
     1441 aaaataaagg taaaaaaaat gtcaacttat gttaaaatct ttttaagaat gggaacatat
     1501 cagattttat ttttagatag tatatcagat tatgcagcag ttaatgaaac tgtaaaatta
     1561 tctaagaaat atgataaaaa atcttctggt ttcataaatg ctattcttag aaatgaaata
     1621 agagctaaag agactataat ggacataact gaggaagata gtgtaaaata cttatctata
     1681 aagtattcct ataactcttg gattataaaa aactggatag ataaatttgg acaagaattt
     1741 gctgaagatt tgttggaagc aaataatgaa aagcctagta tctatataag aacgaataca
     1801 cttaaaataa gtagagaaga acttattgaa aaattaagtg aaatgggtat tgtatgttta
     1861 aaagttccta tggtagaaga agctattaaa gtcgaaaaaa tgaaaaatat agaaaaaaat
     1921 gaattattta aggctggttt atttacaatt caagatataa gttctatgat agtagggaaa
     1981 gtaattaatc caaaagaagg ttcatttgtt ttggatgtat gtagtgctcc tggagggaaa
     2041 tctactcact tagctacttt aatgaacaat acgggtcaag tagtagctag agatattttt
     2101 gagcataaac taaagcttat aaactctact gtaaatagat tggggcttaa aaatgtgttt
     2161 gtagaaagat ttgatgcttc tgagattgat gaaaatagta taagtaaatt tgattatgtt
     2221 ttagcagatg ttccttgctc tggttttggt ataataagac gtaaaccaga aattaagtat
     2281 aagaaagaag aagagttaaa agacatcact tctatacaaa agaaaatttt agaaaatgca
     2341 tctaaatatg tcaaagttgg tggaacttta gtatatagta catgtacaat tcaagatgtg
     2401 gagaatataa atatagttac ttcttttgtt gaagaaaatc acaactttga atttgttcca
     2461 attgatacag taaatgtaga cttagacaat caagataaag gatatttaaa gatatatcct
     2521 aatattcatg gtatagatgg ttttttcata gcaaaattaa agagaataag gtagtgagag
     2581 aatggaaaag aaaaagatag tattaaaaaa ttttactgaa gatgaactta aagaatttat
     2641 gaaaactata gatgaaaagc cctttagagg aagtcaaata ttctcttgga tatataaggg
     2701 tgcaaaaact tttgatgata tgaacaatat accaaaaagt ttgagaaata aattagaaga
     2761 ggtttcatgt atagggaata tagatataga attaaagtta gaatctaagg tagataatac
     2821 aaaaaaatat ttgtttttat tagatgatgg aaatataata gaaactgtga tgatggatta
     2881 tgatagtaga gttactgtct gtgtttctaa ccaagtaggt tgtaggatgg gatgtaattt
     2941 ttgtgcatct acaatggatg gattaattag aaacttagag ccatgggaaa ttttagacca
     3001 agttattaaa attcaagaag atactggtaa gagggtatca aatcttgtat tgatggggag
     3061 tggtgagcca cttgataact ttgaaaatac aaaacaattc ttaaaaataa ttaacgaaaa
     3121 aaatggtctt aatatagggt atagacatat aactctttca acttgtggaa tagtacctaa
     3181 aatgtatgag ttggcagact tagaaatagc tataaattta gctttatcac ttcattcacc
     3241 atatgatgaa gaacgaagaa aaattatgcc tgtagcaaat gcttattcta ttgaagaaat
     3301 attgaatgca tgtagatatt atataaaaaa gacaaataga agggttacat ttgaatattc
     3361 tcttataaaa ggagtaaatg actcagaaaa agaagcaaag gcattagcta agctcctaaa
     3421 aggtatgcta tgtcatgtaa atttaattcc aataaataaa gttgaagaaa gagaatacga
     3481 aaagccagat aaagcattta tttataaatt tagggatagt ttggagaaga acaatatacc
     3541 tgccacagtt aggatgtcta tgggttctga tattagtgga gcttgtggtc aattgagaag
     3601 aaaatacaag taatttgtaa ggaggaagtc gtatggttta tagttgtgcc tcccatatag
     3661 gaaaaataag aaaaaacaat gaagactatt gtgagggaga agttatagat acagaacatg
     3721 gtccaatagg aatatttgcc atagctgatg gaatgggtgg acataaaaaa ggtgaagtcg
     3781 ccagtaaatt agcagtagaa aatataattg attttctaaa ggaaaactta ttacaacatg
     3841 ataatgtaaa aatagactat atagatgata tattaaaaca ggcttataat aatgtaaact
     3901 caattgtgca caaaaaatct atggaagata tagaatttga aggaatggga actacattag
     3961 ttacggctat agtgtataat aatgtcttat atgtggcaaa tgttggagat agcagatgtt
     4021 atttgttaac agatgaaaaa tttgataaaa taactataga ccattccgtg gttgaagaac
     4081 ttatgatggc ccatgtcatt acagaagaag aggcaagaag acatccacag agaaatagaa
     4141 taactagagc aattggaaca gatgatatgg ttgttgtaga tatttttaag aaagaaatca
     4201 aaaaaagtga tataatactt cttgcgacag atggattaac tgggtttatt gatgatgaag
     4261 atataagaga tttaatatta gattatgaat atgagagaac tagtaatata agtgaagaat
     4321 tgataagtat ggccaatgat gtttctggta aagataatgt ttcggttata gtgataaaag
     4381 tataattagg acggtgatat tgtgggagat acaattttag gaaatcgata tgaaatcatc
     4441 aggaagattg gtgatggagg aatggccttt gtatatgagg ctaaagacag attattaaac
     4501 aggactgttg cacttaaagt acttagacct gaatttgtag atgatgatga atttctaaca
     4561 aagtttaaaa gagaggcaga agcagtagca agtctttcac atccaaatat agtaaatgtt
     4621 tatgatgttg gtgaagatgg aaaagttcac tacattgtaa tggagtttgt agatgggaag
     4681 aatctaaaag agattataca agatgaaggt atattagatg aatatactgc attagatata
     4741 acaaaacaaa tagctatggc acttagtgca gcgcacaaaa aggggattat acatagagat
     4801 ataaaacctc ataatattct catatcaaat gaaggaagag tagtaaaagt ggctgatttt
     4861 ggtatagcca aagctgtttc aaattcaaca atgactaata ttggtagtat aataggttca
     4921 gtgcattatt tttcaccaga gcaagctaaa ggtaaatttg taactaataa tgctgacctg
     4981 tattctctag gtatagtttt atatgaaatg ttgataggaa aagttccatt taggggagat
     5041 agtccaattt ctatagcact tcaacacatt aatgatgata tagactttac atcagaagaa
     5101 aaagttagga ttcctcaaag tgtaagaact acaataaaaa agcttacaga aaaatctagc
     5161 gcagatagat atcaaactgc tgaagaattg atagaagata tagagtatat agaaaaaaat
     5221 attgatttag attttataaa agaatatgat gattttgcaa ctaagaaaat tgatgaaaaa
     5281 gaaattaata aagttgtaaa tccaacactt gcaaaaccag ctcctgaaaa agtggttaag
     5341 cctgttgaag tagctgattt ggatgatgat gaagattact atgatgattt ttatgaagaa
     5401 gatgaagaag aagaagaaga agaggaagag ataatgagag ctaaaaagaa tcagagacct
     5461 aagagtactc caagtaagag aactaagaaa aagaagaaaa aacaggaaag cccaaaatct
     5521 agaagaagat taaaagtaat tgcggctgtt ttgatactga tattgtgtgc tcaagtattc
     5581 ttagcatata agtttttatt tgctggaggg tttggcaata aatctttgac tgttcctaat
     5641 cttgtaaata tgacattaga agaagctcaa agtgctgttg aaaaagaagg attatacctg
     5701 agtgtaaaaa gtgaagagta taatagtgaa gttgatgaaa attgtataat atcacagaca
     5761 cctgaaggtg gaagtacaaa tgtcaaaaag ggtgatacta taaatgttgt cgtaagtaaa
     5821 ggttcaagtc aggcatctgt tcctaatgtg gtgggactta ctttgagtaa tgcaaaacaa
     5881 cttattgaag aaaataactt aaaagtggga actgttaagt atgaatatag ctcaatatat
     5941 aaagaaggta cagttttaag tcagagtcca ggtgctggct catcaagagc tcaggaaggt
     6001 gatgaggtaa acctatatgt aagcaaaggt tcagaaaaat caaatacaca gactccaaca
     6061 gtaccagata aaaaaccaac aacaccagag aataatacac caacagaacc tggttcaaac
     6121 tcagggaaca gtggtggaaa ttcaggaagt tcaaactcag gaaatagtgg aggaaactca
     6181 ggcaataatg gaggtaactc aggaggttca aactcaggaa atagtggagg aagctcagga
     6241 ggctcaaact caggagataa tggaggaagt tcaggaggtt caaactcagg agataatgga
     6301 ggaagttcag gaggttcaaa ctcaggagac aatggagata gttcaggagg ctcaaactca
     6361 ggagataatg gaggaacttc atcaggaact ggcgcaaaca ttggtaaaac agaataaata
     6421 ttaataaaat aagagctgtc cttttataga aaatattttc tttaaagggc agtttgtttt
     6481 gtataaaata ctgaattggt tataatataa ggagaaaagt atttatgaag aagaaaaccg
     6541 ttttaattac aggaggagct agagggattg gtaaagctat gtctaaagct tttgcaaaag
     6601 aaggttacaa tgtattagta aattttaata agtcagaaaa tgaagctaaa gagttgtata
     6661 ctatattaaa tgaaaaaaat ttttctgtaa aactttttaa ggctgatatt tcaaatagag
     6721 aagatgtaga gaatatggta gattattgta taaaagaatt tggaggattg gatgtacttg
     6781 taaacaatgc tggtgtaagt caagataagt tgtttacaga tataaccgat gaagattggg
     6841 ataatatgat gaatattaat ttaaaaggaa gcttttattg ttcgcaagtt gctttaaaat
     6901 acatgatatc agaaaaaaaa ggaaatataa ttaatatatc ttcaatctgg ggtatatcag
     6961 gtgcgtcttg tgaagtgcat tattctataa caaaagcagg tataataggt atgacaaagg
     7021 cattagctaa agaagtagga ccatcaaata ttagagtaaa tagcatagct ccaggtgtca
     7081 ttaatactga catgttatct ggatataatg aggaagatat agacgctctt gtggaagaaa
     7141 cccctctaat gaggctagga actccagaag atatagcaaa ttgtgctatt ttcttggcat
     7201 cagacaaatc aaattttatc actggtcagg taattagtcc aaacggagga tttgttattt
     7261 aaaaaaaatg tggtattata taaaaatagg aaagaataac acgatatact attcataaac
     7321 agtatgtgtt tatataaatt ttataaaggg aggcatttga tgctagaagg aaaaattata
     7381 aaggggatta gtggatttta ttatgtagat acttataatg gtatttatga atgtaaggct
     7441 agagggatat ttagaaagca gaaaataaca cctttggttg gtgatagagt caaaataagc
     7501 attgttgatg aagatgaaaa aaagggtatc ttagaagaaa tcgacagtag agatacagaa
     7561 cttataagac ctcctattgc aaatgtagat aaagcattaa tagtattcgc aataaaaaat
     7621 cctaaaccaa acttatctct tttagataga tttatagttt tggcagaaaa agaaaattta
     7681 gaaactgtaa taatccttac aaaagcagat ttagatgata atgatacatt agaaactgtt
     7741 aaaaatatat atgaacttag tggctataaa gtcattcctg taagcaatat tactaagtta
     7801 aatatagata aagtaaaaga agaattaaaa gaaaatgtag tagtgtttgc aggaccatct
     7861 ggagttggaa aatcaagctt attaaatgaa atagatgaaa attttaaact tcaaacagga
     7921 gtggtaagtg ataaaataaa aaggggaaaa catactactc gtcacgctga acttttaaaa
     7981 ttggaatttg gtggaatggt tgcagataca ccaggtttta gttcccttgc acttgaagat
     8041 atagaggaag ttgaacttaa agattatttt atagaatttg ataaatttaa tgattgtaag
     8101 tttggttcaa agtgtattca tgaaaatgaa cctaattgtg ctataaaaga agctgttaca
     8161 aatggagaaa tatctaaaga aagatatgat agttatatac agctgctaca tgaaataaga
     8221 caaaataata gtaggaggta ttagaaaaat gattaaatta gcaccatcaa tattatcagc
     8281 agattttgca aaattattag aagatgtaag aaaagtagaa agtgcaggat gtgaatatct
     8341 tcatatcgat gtaatggatg gacactttgt accaaacatt acattaggac cacttgtagt
     8401 caaaagttta aagaaagaaa atataaatat ggtatttgat gctcatctta tgatagaaaa
     8461 tccagaccag tatattgaag aatttgtaaa agcaggatgt gatataataa ctgttcacca
     8521 ggaggcttgt gttcatctac atagaactat acaaaatata aaatcacatg gaataaaagc
     8581 tggtgtagta cttaatccag ctacaccagt agatacgatt aaacatgtac tacctgattt
     8641 agatatggta ttattaatgt ctgttaaccc tggatttgga ggacaatcat ttattccttg
     8701 tgttttagat aagattaaag agttaaaagc tattatagat agtcaaggat taaacattga
     8761 tatagaagtt gatggtggta taagccctaa aaacgtagcc gaagttgtac aagcaggagc
     8821 taatgtaata gtagcaggtt ctgcaatatt tggaagtgat gacattcaag aaacagttaa
     8881 tttatttaga aagaatgctt ctcttgaaga attagtttaa atttaataat gaaaatctgt
     8941 attgttttaa atggcgaaat tgaagattac gaagttacaa gagatattat aattaaggaa
     9001 tgttatgact gcataatatg tgcagatgga ggggcaaatc acacttataa aatggaaata
     9061 atgccagatt atatattagg agatttagat tcagtagaag aagaaaagat aaacttctat
     9121 aaaaataaag gtgttaaatt tgaaaaattt ccatctaaaa aagatgagac tgatacagag
     9181 ctttgtcttt ttttagctaa gacacttaaa gcaaatcata ttgatttttt tggtgcttta
     9241 gggggaagga ttgaccacac tcttgcaaat ataaagctat tatattattt aaaagaagat
     9301 ggtatatatt ctaggatact ttctgataaa gaagagatgt atatagttga aaatgaggaa
     9361 atttcattgt atggaaaccc aggggacact gtttctgtaa cagctataaa aggtgatgct
     9421 aagggtgtaa cacttactgg cttagaatac cctctcgata attattatat gaaatactct
     9481 gtacctattg gaatatcaaa tgttatgttg agtaattctt gtaagattaa agtagagcaa
     9541 ggctgtgtac ttgtagttag aaatttataa aatttgattt tgacaattgg ttttatattg
     9601 tagagcagat acaatcaagt aaaatatatt tatgattgta tctgcttttt agtatataat
     9661 tataaatata taataaactt tgacataagg gggatttttt gtggtagata gtattatttt
     9721 tgatttagat ggaacattgt gggattcaac tgaagaagta tgcaaagtct ggcaggatgt
     9781 tttaagtaaa cataaggaaa tagaattaag tgtcacaaaa gatttattta gaagtcttat
     9841 ggggctttct tttgaggaaa tagcaaatcg tttatttcca aatttaagtg aagaagaaag
     9901 aatgaattta ttgaatgaat gttctttgaa agaatgtgac tatttatctg aaaatggagc
     9961 taaattattt gatgatatag aagatactct taaattacta tctaaaaaat ataaattatt
    10021 tatagtaagt aattgtcaag ctggatatat agaatctttt ttaaaagcac ataaactaga
    10081 acagtatttt atagattttg aatgtcctgg taatactggt ttgcataaag gagaaaataa
    10141 taaacttata attgaaagaa ataaattaat aaatccaatc tatgttggtg atacgcaagg
    10201 tgatgcaaat agtgctaagt ttgtaggtat accttttata tatgccaaat atgggtttgg
    10261 gaatgtagat gtatatgatt attttataga ttcatttaaa gatttgttag aacatgatat
    10321 attaaaataa gatgagttta aaaattatat taaaataata aaaaagttat ggggggattt
    10381 aaaaatggaa ttacaagata ctatttttaa aagacaaagt gtaagaaagt ttaaaaatca
    10441 agatgtatca gatgaagata ttttaaaaat gataaaagca gcaggtgcag caccatcagg
    10501 caagaatatt caaaactggc attttgttgt tataaaacgc cgtgatttaa tggaaaagat
    10561 agcagatgtg ataactaaaa agcaacaaga aatacttgtg gaaatggata aagtatcagt
    10621 ggataaggct aatagattta gaaaatttgt taaaaacttt acactatttt acttaaaagc
    10681 tccagtttta gtattagttt ttacaaaggt atataatcca tcaggatatt atgaattaga
    10741 gcttatagat gcacctaagg agactataga taaattattt ataagaaatc caggtatgca
    10801 gagtctaggt gcttcaattg aaaactttac actatcagca attgaacttg gatatggttc
    10861 ttgttggttg acaagtcaaa actatgcagc agatgagata gaagctgttt tagaagcaga
    10921 aactggtttt gaaaagggag agtatttttt aggtgcaatg ttagctcttg gagtgccaga
    10981 ggacaatttg aagagtccat ctaaaaaacc agtagaagaa atatgtacat ttataaaata
    11041 aaagtcaagg acattttaaa atcaaagtaa attaaaaaca agataaatta aaaataaata
    11101 tttaaaaaat taaaaagtag gttttattga tattaatttt atatttcaaa tcattgattt
    11161 aattaataca ataagaacta ctttttttta tatattaatt ctaaatatat ttaaatcttt
    11221 agaaaatctt tagaaaagta tatgtaatat cttaagtttt taattttaat attaagtata
    11281 taaaacaaaa tgaaaaaata ttaagtttaa actagaagga ggtttctaag atggacatta
    11341 aatacaaata accagaacaa aagatataaa tatatatata aatggaaaaa cttctatcat
    11401 attgtataat catatatgta aagaaaattt ataaattaat ataaaggaga ttaaaatgaa
    11461 ttcatataat attttggtgg tagaagatga aaaggagatc gcagatgcta tagagatata
    11521 tctattaaat caaggatata atgtttttaa gggatacaat ggattagaag gtcttaaggt
    11581 tatagaaaac caagaaatac atttagctat agttgatata atgatgccta aaatggatgg
    11641 tataacactt acaatgaggc ttagagaaaa ctataatttt ccagtaataa tgctatctgc
    11701 aaaatcagaa gaagttgaca aaataatggg gcttaatata ggagcagacg attatgtaac
    11761 aaaacctttt aagccattag aactattggc tagagttaat tcacaactta gaaggtacac
    11821 taagtatcta aacatggttg gaaataaaga gcaaaaggta gaaagtgatg atagtgtgtt
    11881 tgcaataggc ggattagaat taaatgaaaa tacaaaagaa gttaatattg atggaaaata
    11941 tataaaaaca actcctatag aatttaaaat actaagttta cttatgagaa atgcaggaag
    12001 agttttttct gctgatgaaa tatatgaaag agtttggaat gaaaatgcta taaatacaga
    12061 tacagttatg gtacatgtga ggaatataag ggaaaagata gaaattgacc caaaaaatcc
    12121 taaatattta aaggtggtgt ggggtgttgg atacaaaatt gaaaaaattt aaagataaat
    12181 atatagttac tattatagtt tttatactta tgttatcagc atcattaggt atgattagtc
    12241 aatattctac tatacaagca ggtgctaaag gtggagcaaa gaatccattt gaacaggaaa
    12301 gctttgtaga tagtatatat aaaggaagtt atgttttata tcataatatg aaggaagaac
    12361 aagaaggtaa gatgatacaa ccatcaaagc tatttatcaa tgaagaaact atgaactata
    12421 taaaaaatgt aagtaaagaa aaagatactt atgatggttc agaaacttat tatacagatg
    12481 atagtattaa tgcagatttt aatgaaaact ttgaaaattg ggagcatttc attaaagcgt
    12541 caggaaagaa tttaaagtac tatttagttg ataaagaaag caattttgaa ttttttaatg
    12601 ataataaaga attaggagta cttgatacaa caaagaatga tgcttcaaaa gaaaattctg
    12661 atgaaaatca gtcaaaaaat acagaaatga atgatgagga aagacaaaag cagattacag
    12721 atataaagga aaactataga ttctacgttg taatgaattt tgataaagat ggaaaagtaa
    12781 atattgtgga ttcttatgga gtaaatcaaa aagttattaa tcaaatgttg ttatctaaag
    12841 caagagaaga tttaatggat acaagtttag gaggaaattc aatttcatat aagttatctc
    12901 caataaaaaa tataacatta gtatatgctg tacctaaagt aataagtaaa ggtgatagtg
    12961 tagagtatgg ttatggaaat acttattttg atgatggtat aatttcatat ataagcaatg
    13021 tagaaaataa ttcttattat aagatttctg gaatgatttt aaaaattata ttagctatag
    13081 ttgttattgt agctttagca ttcccatata gaaaatctaa agagttgcta ggatttgata
    13141 cattattaag aataccgtta gagatactct ttattgctct tgctattata caggggcttg
    13201 tagatttata tcctactgtt ataataagta ataccttgag tgggaattat gtagagtacc
    13261 ttataaacaa tgacataagt gctaaaatgg ctagcatatt ggtaagttta atgaatttaa
    13321 cttattggtt tgtaatattc tcaataatat ttgtatttat aatactttta aaacatattt
    13381 taaatgttgg aataagagag tattttacaa aacatactgt gacaggaatg ttttttgtat
    13441 ggttaaaaaa agcaggaaaa acagttatta atcaaattag aacaataaat ctaaaagaaa
    13501 agccaaataa aactatagtt acaattttgg gaataaattt attgattata gttataatgt
    13561 gtagtatgtg gttctttgga ataatactcg cattaatata ttcagtagta ttatttaaga
    13621 tactgtcaga ttactctaaa aagacaatat atgaatataa tcaactttta gatgttacaa
    13681 agaaaatttc agatggaaac ttagatgtaa atatggaaaa agatttagga ttttttaatc
    13741 ctataaaaga cgaattagga aatattcaaa ctggatttaa aaaagctgtt gacgaagagg
    13801 ttaagagtca aaagatgaaa acagagctta tatcaaatgt atcacatgac ttaaaaacac
    13861 ctctaacatc tataatcact tatatagatt tattaaaaga tgaaagtata acagatgaaa
    13921 atcgtaagtt gtatattgat acattagaca gaaagtcaca aagattgcaa catctaatag
    13981 aagatttgtt tgaagtaagc aaggcaaata gtggagatgt ttatttgaat atagtaaatg
    14041 tggatattgt atctctaatg aagcagacac ttttagaatt agatgacaaa cttgcagatt
    14101 catctctaat agtaaagaat aacttttcta atgaaaaaat aatattacca ttagatagtc
    14161 agcgtacatt tagagtattt gaaaatctta taataaatat aagcaaatat gcaatgccaa
    14221 attcaagagt gtatattgat attttagaaa cagataataa agtaaatatt atgcttaaaa
    14281 atatgtctgc atctgagatt gattttagtg tagatgatat aatggagaga tttgtaagag
    14341 gagataaatc acgtaacaca gaaggttctg gtcttggttt agcaatagca aaaagtttta
    14401 tagaattaca gggtggtaag atgaatataa gtgtggatgg tgatttattt aaagttacaa
    14461 ttacttttaa caaaaattag attagacttc tttaataatt ataaagtcta tagaataaaa
    14521 gagtctggaa atatttatgt gtattcttta aataaaaatt tataggtatt gttttaaaat
    14581 aatttgtaag tattatttca aaacagctta taaatgttat tttaaaacag aatgttgatt
    14641 tatttaaaaa aggtgaatcc taatatacaa ggttcacctt tttattttac aaaatgttat
    14701 ttagtgttac agagcttata aatagcataa tatctgggtt tactattaac atagtactag
    14761 caatttattt tgaaatcttg tataagctat tttatattat ttaattacta ttgtttgtac
    14821 tttacaatgt gataaagaat ttacctctac agttttagta tttactttaa gcttgctatt
    14881 attatcatag atttcgtcga gcattgcact ataagcatca ttgacatttt tattgatgtt
    14941 aagacttccc ttagcatcct catttttcat accattgaag attctaacta caattccatc
    15001 tcctttttct tctttcttta ttgcacttac tgtgaaattt gaattattta cattcatcaa
    15061 actaaattca ttttcatata tttgtttttc gtcattgaat gcaaatagaa gtctaccatt
    15121 taaaaaatca gcatattcat atatttgtat aggtgttaag aatctcttag ctgtattagc
    15181 tacatttgct tcatcaaatt tattgttata tatgtaaaga ccaaagtcaa agtttaactc
    15241 tttaagtaat tgtgcatctg gtgttgctat tattttttct ccagaagctc ttcctggtct
    15301 ataaagtaaa ttttctcttc ccatatatcc aaatgacctg aatagtgtta atgctattgt
    15361 atctaaatta tcaccaacta tttgatattc tcttacacaa ccagttatta atgctattcc
    15421 tctttgtcca tcctctaaag taacaaaact ttgcattggt tcgatagtta taggtttttc
    15481 ttgccatgag taattgtctc ttttccataa tgcaaggtct ttttctaata ctgttggtct
    15541 ttgtattatt ccaaattgtt gatctgctat agaaaactta gatgctattt ctgtattaaa
    15601 taaaactctt actctatgag atagtgcttg gttatttttt atattaacat taaacttaat
    15661 tatttgttct ttaggttcta aggtaatttt cattgttact ggcattttca cagaaacttt
    15721 acctatagac ctttcttcca gtgaagctgg tatggtcaat gaatatttaa gtatcatttc
    15781 ttcttgaata tttgatttta gcacttctac tgaagataca gaatctaaag atgaaacata
    15841 aatatctttt cttggaggag aatagttgta tgaatctcca tcatctccat tgtcttcaat
    15901 tacagcttgg tttttataag tcttactaga ttctttatct aatatttcta atgaaccatt
    15961 tttattggct gttattttgt aatatttgtt ttctatttct ttgcaattac ttgtttctat
    16021 ttcacattta tttttagtat ccaattcaaa aactagttga gtatatccca ttggtggtat
    16081 atcttttact gaaagtgcaa tttttgctct atatacagtt ttgggtatat atattttttt
    16141 acttgggttt aatcttattg tttgagttaa tatataattt gtaatatctt ctttttcttt
    16201 tattatataa ttaagaattt ctccattttt attctttatc acaaaatctt gacctggaat
    16261 ataagtgtca aattctacaa cttcatttct gacttctggg aatccattga atactgttaa
    16321 tgttatttct tcattattat ttttgatttg agtacttata agtctcatat gcaaatctaa
    16381 taaatttata ctcaaatctc tggcttgttt gtatcttgtg tatacatcct catttgctgt
    16441 gtcactatta caagaaccta tgctgtcatg agctgcattt tcaaacataa gtttccatat
    16501 atcttttata actagatgag ggtattcatt tccaagtgaa taacttaatg ttaataatgg
    16561 ttcaagtaca tttgtgatat aattttctaa tttgttattc attattttaa ggtctgacct
    16621 tgaggagaat atggatttgt gtattctcat atgtttagca tttaaaaatt cacctttaag
    16681 ctctgtaaat tcatttcttt cattttttac tgaatctata tagttttcta tattacttat
    16741 ttcgtattca ttttcattat ctatttcatt agcttttttt aatatatcag gaaggttttt
    16801 tcttattggt gcttggtcaa atccatttgg aaaatatacg ttattagtag aagctttaac
    16861 cttaagtttt ccaattatat cttttaaata ttcttttaaa tctgaatcat cttctggtat
    16921 attacctcca tagtaataac caaatggtat ttgaactgct aatacttttg aaccatcaga
    16981 acctttccat ataaactctg ttgtatctac catatcatca gaaactccac gccaaaataa
    17041 tgaactatct attccaaaac ctctgtatat ttgtggcatt tgagctgatt gcccaaaaga
    17101 atctggtaca tatccaaccc tcataggttc accaaattca ttgcatctat ctattccata
    17161 gtaaaggttt ctaattatag attctcctga tataaccaat tggtctgttt gagtatacca
    17221 aggtccaata actaatcttc ttctttgaac taaatctctt attctatatt catcctgagg
    17281 tctccatttt aaataatcat ctaagagaga agcttgtgca tccatagtaa agtatttaaa
    17341 gtcctctttt tcttccaata tgtctaatat atcttgaaaa tctttcatta aatatatttt
    17401 tgaccttgat gttgtaaaat accattctct atcccaatga ctgtgtggaa ctacatgtac
    17461 ttttgtcttt ttcataaaaa tcctcccaaa atttaattga gaattgagag tacactcaat
    17521 tctcaattat ctaaaattaa gtttttaatt tacattatat ctaactctat ttcatcaaat
    17581 tctgtatttt ccgtagtttc atcttcattg aagtctacta aagttgttga taatattgct
    17641 attactaaag ctccaaatat tgcacctact aagtaagccc atccagaacc tattgatata
    17701 aatccatata atccaccaac tggaggcatt atagcatgta cacctaacaa agctgataat
    17761 cctacaccca aagcgcatcc tatcatatta acaggaacta attttaatgg atttgttaaa
    17821 gtgaaaggaa ttgcaccttc tgttattcca attataccca taactatatt gccttttcct
    17881 gtttcagcta atgcattgga aaattttgat tttcttatga atgtagctaa accatatcct
    17941 agtggaggta taactattgc gcagtttacc aatatcgctg gtagatagat tccctctaac
    18001 aataatgcat ttcctgccat ccaagctgct ttatttactg gtccacctaa atcgaatcct
    18061 atcattgcgc ctaaaattaa agccaatacc accgaatttg ttccattttg acacatttca
    18121 gctacccaag catttaatga agtatttatg tttcctaaag gtcctgctaa tacaacactc
    18181 attaaaaaac cagttaatcc aactgttata agtggcatta ttataagtgg catcattcct
    18241 gatgctgacc cagatatttt aatttttttc tttaaaaact tacaaacata cccagcaaag
    18301 aaacctgcta ttactgctcc taagaatcct gcattagtat tacttgctat gagcccacca
    18361 ataaaacctg cacccatagc tggtttctca cctattgaaa atgctataaa tcctgctaat
    18421 accgtattta atagacctat gccagtccaa ccaacttgtt ccattaagta aagaatatga
    18481 taaaaaccag atacattttt ataagcatct aaacttgtta tacccattgc taaagctgta
    18541 agctttggga tagctacaac taaagatgag cctattacaa gtggcaacat atatgagata
    18601 cctgtcatta agtgtccctt aagttcactt aaaagagatt tatttttttt actcattttt
    18661 aatcctccta tataagtttg ttatattttt tgataatatg ttataattac attagacaat
    18721 tttttaattt tttatttttc tcattctata ttagagtgag atttttttat taagaaatag
    18781 cttctataca ctttgttaag acagcttctg gtttttttaa cgctatacct attttgactt
    18841 ttatcacttt tttaccatca aatctttcca ttccagatat agtttggtca gatgctataa
    18901 gtataaagtc cgcatcttgt gcttcttctt ttgttatagt atttactcgt cccatagcac
    18961 cttgttgttc tactttgatt tcatgaccca tttttgatgc agcttgttct aaagcttttg
    19021 ctgccatagg tgtgtgtgct aatcctgcta tacatgatgt tattgctact attttcataa
    19081 ttattcctcc ttcattttat tagttatata ttttactaaa tgacttgcat cactagcatt
    19141 tttaacttca cttgtaaaat catcctccat taaacatgtg gctaatttag atagagtttt
    19201 taaatgtata tttccttcat ttttatgtgg ggctaacaaa ctaaatacat atctaacctt
    19261 actatcatct aaagttttcc actctaactc gttagttgtt ttaatataga gtatggaagc
    19321 ttcctttaca tttacacttt ttgtatgagg tatcgcaaat ccatctttta ttcctgttgg
    19381 aaattcattt tctcttttta gaaggtcttt taaaagagcc tctttattag ttgcaatacc
    19441 tagtatataa gcactatctg caataaaatt taatacttct tctttgcttt ttaagtcttt
    19501 atctataaat atatattttt cttgtagtac catatatttt tccactccat tcgtaatgtc
    19561 ttaacttata tttatattgt aatcattatc ctaattaaat taaatataac taatttccac
    19621 ttcgacatgg aaatttatat tatgataata actaatttcc atatagaagt ggaaagaaat
    19681 tattataata caattttatt agagtaattg tattatatga gtgtaaatag tataatttag
    19741 aagaaattct ataagggggg atatttatgc acaagagatt gctcacgttg tttaaattat
    19801 taaatgaaag tgatgataaa attacttgta aaactttaag taatcattta aaagtgagtg
    19861 agagaactat aagaaatgat attacttcta taaatggaac tttagagaaa aatggtgcta
    19921 ttattaaaat aaaaaaagga gaaggatatt atatagatat tcttaattta gcattatacc
    19981 aacagtatct agcattaata tctgatgata ttatggattc aagtgaaata ccagattctc
    20041 ctatagaaag aaatcaatat attttaaaat atattctcta taacaataca tatataaaac
    20101 tagaagattt agctaatagc ttgtatgtga gtaaatttac aatactaaat gacataaaaa
    20161 gaataaagcc tattttgtca aagtataatt taatattagt ttctaagcca tattatggag
    20221 ttaaagttga agggaaggaa atagatattc gaagatgtat ttctaataat atgataaata
    20281 ggaattttga aaactatata attggaatta cagatagaga aatagaatta tttaataatg
    20341 tagatttaat agagcttcaa agggtagtgt taagtgaaat taataaattt aatataaatt
    20401 ttctagattt taatctaaaa aactttatta tccatttagc tattactata agtagaatac
    20461 ttgatggtta ctgcttagat aatgttttag atgttgtttt aacagatttt caatcgaata
    20521 caactgttga aaatatattt aattatattg agagtaaata tactataatt atttcaaagg
    20581 cagacagagt ttatctatat aatcatttta taactaaaag ttctcttttg gataacgttt
    20641 ccaatagggt ggatactaaa attatagagt atgttgaaga aattttagaa gtaatcaaca
    20701 atcaatatac ttttgacctt agaaatgata gtgtattatt tgatgattta gtactgcact
    20761 ttaaatcaat tctcaattct aaatcttata atttaaataa ggtgaatccc cttataaata
    20821 caataaagag taattatcct ttagcatttg aaataacttt aaatgctata gaaaaagttt
    20881 ttaaaaatag tatttactct ttaacagaag atgaaattgg atatgtatcc ctgcatatag
    20941 gtgctggaat agagagattt tttcaaaata atattaagtg taaaaacgta gtattagtat
    21001 gtggttcagg gtatggttca agtaggttat tagaggttca attaaacaaa gtttttcatg
    21061 ataaaataaa tatacttcaa tgtctatcat ttaatcaatt tttagctagt gaattgtcag
    21121 atgtagatat aataatatcg acaattcctt taaatcatga tagtattcct attgtattag
    21181 tggatttaaa actattaaaa aaagatatag agaatatatc taagtcaatc actaataatt
    21241 cccatattta tagtaatctt ttggacaatt tctttgataa aaatttattt attgttaatc
    21301 ctaaaatcaa agataaagat gagcttataa agttgatgtg caacaaatta actcaaagtg
    21361 aaattgtatt tccatcattt gctgaatctg tattttacag agaaagttta tcttctacca
    21421 atatagatga ttttttagct atacctcatc ctatggaatt aagctctata agaactaaga
    21481 tttgtatttc catattgaat gaaccaatat attggagtga agattctact gttaaattaa
    21541 tcatgatgtt agctataaat aaagatgatt atataaaaat aaactccatt tatgatatcc
    21601 ttttaaagat aattcatgac aatgatatta gagatagtat atctaattgt aatgattttg
    21661 ataattttct ttccataatc aagtctatag tatagatttt tatattacat aagttttaaa
    21721 aactttaata aaatattttt tgtttttagc ataaatagta taagtaagtt ttgtataatt
    21781 tgttattatt ttgtattgat tataattatt atacaataaa gattctgtct caaaatagag
    21841 agagaaactt gtttttcatt gataaaatga taaaaatata tttgatttca tacaaaaaag
    21901 agtgtctcat gaactaaaaa gttcattttg agacaccacc tttattattt tatattattt
    21961 atctcttgta tatattacta tagcatccct taaaaactta gctaatccag gttgttctct
    22021 atcataatat gatgtaaaac gttcatcata tacatacatc tgtgctagag aggaatgagc
    22081 ttctttagag tatgtgttcc aagtgtaact caaccattta tgatgtaatt ttgcaacttc
    22141 ctgagccagt tcactagagg catcaccagt tttaaaagct tttttaagtt tactaattat
    22201 ttcgatattt aggttttgcc aattttcata atcttgctta gacatatttt taagcttttt
    22261 atttgatgta tctataatat ctttaccata tttctctcta atttcagttc cataattttt
    22321 ttcattttca tcaatcatct tttctttaaa accttcaaat ttttctatat ctgtcatagt
    22381 gtattttccc tccttcagag ctatagtttt agttacattt tctattagta aatcaatttg
    22441 ctttcttttg gctagtaatt tattatggtg ttcttttaac gcacttaaac tatcgaagtt
    22501 tggtgaattt ataatgtttt ttatattttc taagttaatt cctaattctc tataaaaaag
    22561 tatttgttga agtttattaa cttcattttg accatatata cgataacctg aagaatttat
    22621 tttaagtggc ttaagaagac ctatttcatc ataatatcta agtgttctag tacttatacc
    22681 agctagttta cttaattttt gaactgtata ttccataagt aatacctcct ataaattaaa
    22741 tatacacttt tacgcaacgt aaatgtcaat atattaaaat ataaaaattt atatctttta
    22801 ataaaaatat aaaaaaatat ctttactgga tattttacac acaactgtca atactcacta
    22861 atgaaaatta aaaaataaaa tttatagact atctttacta aaatatagaa ggtctatagc
    22921 ctaggaggaa ctgtatgaat ttaaaaagaa gcaatgaaaa acgagaagag tacagaagaa
    22981 tgtattcaga taaagaaagt tttttaagtt tgattcaaaa ttttacaagt gtctcaatag
    23041 caaaagaatt aactcttaaa aatatagagt tagaaacatc ttttatatgt gaatataaag
    23101 gaaaagaagt tgatataatt tataaggtat tttctaaaag tggcaaagta tcacattata
    23161 tagtgttaga atttcaaaca gaaatggata ctgaaattgt cccaagattg aaatcgtata
    23221 gagaacaaat ttggaaaagt tttattatga aaaaaagcct tgaagaaata gaagataaaa
    23281 attttaaact tcctaaagtt ataccagtgg ttttatatag tggacctgaa aggttaagta
    23341 gaaaaaaagg aattttggat attgtagaat ctatatcgga gttcagtaat aattctagta
    23401 tgaaaaatta aaattacata atgtgtttat tggtctaaaa tggaaaactg tcgcataaac
    23461 ttgaatatga aaatatttat agatacaggt tctatgtgga ggtgttccat gagaaataac
    23521 aagtctaagg tattgctagg aatactttgt ctagcaacag gaatggctgt aattctgtca
    23581 atgttgcttc ctggctggat ttggtcagca cttaccgcat taatacttat tggatgtgga
    23641 gcattattat ttttttgtta aaatagtttt aactataaag tgtggggtga gtattgatga
    23701 aagtagtaac aataaaatta ccaaaatgga tttcaagtat aatcctaaaa tttatgagaa
    23761 agagtaaaaa tgattaaatt aaaaagccta cttacggaag taggctttta aacgtggtta
    23821 ctaagctctt tcaattttac cagaacgtaa acatctagta caaactttta ctctcttagg
    23881 agctccatct ataatcgctc ttacgcttct taagttagct gaccatgttc ttttattatg
    23941 tttatttgag tgtgatactt ggtttccaga aaccttacct ttatcacata cgctacatac
    24001 ttttgccatc tacgcacacc tccttaaaat gataactatt taaagactaa aaacatattt
    24061 atattatcat aaataacaaa aatttgcaat agaaatatat atttgcctta cacatattga
    24121 agaatttctt attatcatat aaaattatat atataatttt atataaagta aatatatttg
    24181 gaaaataatt attatttttc tttgaatata tatactatag aagaaatttt atccaaaaac
    24241 ttttctaggt ttaaattata ccatatttat agtatgatat gattataatt ttatataata
    24301 aataagtaaa aatagtaaaa aataaattaa gaaatgtttt taataaaaat aagcttaagg
    24361 gggtcaacca tgagtgcaaa ggtaacgaat caatatggaa atatagaaat agataatcaa
    24421 gtaatagctc aagtcgtata tagagctgca atggaaagtt atggcttagt aggtttagca
    24481 tataagtcta aaggaatagt agagctatta aaaggcgaga acgcaactaa aggtgtaaga
    24541 gttgaggaag tagaagaaga tgccatagca atcgaacttt atgttataat acaatatggt
    24601 acaaatatat ctactgttgc caataatata atagatagag taaagtatgt agttgaaaaa
    24661 atgactggcg taagagttac tagaattgat attaatgtac aaggaataag agttaagtaa
    24721 tttcatagga ggaaacaaat gattcaatat atagatggta aaaggttaag ggagatgttt
    24781 atttcaggtg caaacaatct ccaaaacaat aaagagttag ttgacaagtt aaatgtattc
    24841 cctgtaccag atggagatac aggaactaat atgtcattaa caatatctta tgccttaaaa
    24901 gaattagcaa aagttgaaaa tgataatata tcagatatcg gaaaagcatt atctaaaggt
    24961 tctttaatgg gagctagagg aaactcagga gttatacttt cacaaataat cagaggtata
    25021 gctaaatcta tagaaggaaa aagtaaatta tcaacagaag atttagcaaa agcttttaaa
    25081 aatggttcag acacagcata taaagctgta ataaaaccaa ttgaaggaac tatacttact
    25141 gtagttagag aaagtggaga gtttgcaata aaaactgcga aaaaagagaa agatgtagta
    25201 aagtttttaa gtatgcttgt gaaggaatca aattcttcat tagaaagaac tccagacctt
    25261 ctaaagaatc taaaagaagc tggtgtagtt gactctggtg gaaagggtct tgtacttata
    25321 tatgaaggaa tgttagcttc tataaaaggc gataacatag aaataaaaaa tgttgaccta
    25381 gatactaata tttcaacaag tatggatttt gcaaagagta ctactagcac agatgatata
    25441 aaatattgct attgtacaga atttatttta gaaagttcta aggtagaaga tactaaaata
    25501 agagacataa tgatggctta tggtgatagt ctagcagtag ttggagatga tggagtcata
    25561 aaggtacatg tacacactaa tgacccagga aatgtacttc aagaagctct aaagtacggt
    25621 caattattaa ctataaaaat agaaaatatg aagttacaac atgaaaatac attactggat
    25681 gtagaagaaa agaaagaaaa tgatagtgaa cctttagaag aagagaaaga gtttgggttt
    25741 atagctactt ctatgggtga aggtttggca aatatattta aagatttcgg agttgaccat
    25801 ataatagaag gtggtcaaac tatgaatcca agtactgaag attttatgaa tgctataaag
    25861 catataaatg ctaaaaatat atttattttc ccaaataaca gcaatataat aatggctgct
    25921 aatcaggcaa aagaattgag tgataaaaat ataatagtta tacctacaaa aaatacgcct
    25981 caaggatttg ctgcattagt aacttttaat ggagaattaa gcgaagatga aaataaagaa
    26041 gctatgatga atgctttaaa ctcagttaaa tcaggtcaag taacttttgc tgttagggat
    26101 acagtgatga atgaaattga tgttaaagaa ggtaatataa ttggaattgc agaaggaaat
    26161 ttattatcag caggagatta tgtggatgaa gtaacatcaa accttattga aaagttggtt
    26221 gatgaagata ctgctataat aacattattc tttggtgaag atgttacaga atctcaagca
    26281 aatgaactac gtacttcttt agaagaaaag tttgaagatg tagatgtaga gttatattat
    26341 ggaggacaac ctctttatta ttacttaatt tcagttgaat aatgattata agaacctgta
    26401 tgaccaagta gcttttataa aattggttgt acaggatttt tgtgtagtag gtgatatatt
    26461 tgttggattt atataaagat gttcaatatg ttaagggaat aggacctaaa aaagcagata
    26521 aattaaataa actgggtata tttacactaa aagacctatt gtattatttt ccaagacaat
    26581 ttgaagatag aaataatcta aagaaaatag cacaattaga agatggtgag aaagttacga
    26641 taaaagcagt aatatcaagt ataaatacat ttagcccaaa agaaggtatg actttaacaa
    26701 agattgatgt aaaagatgag acaggctcgg ctaaattagt gttttttaat aaaagttata
    26761 taaaaaatac ttttcgtcca ggagattcaa tattagtttt tgggaaggtt aagaaaaaat
    26821 ttaataactt agaacttaca tcatgtgaat tagaatatct tactaattca cctaagaata
    26881 catgtagatt tatgcctgtg taccagctta catacggtgt tacaaataaa gaaattatga
    26941 gtataattag aacagtactt gaagataaag aattaattat acaagaatat atgccacaaa
    27001 gaattataga aaaatatagg ctatgtagta tagattttgc agttagaaac atacattcac
    27061 caagtagtaa ggaatcatta aaaatagctt tatatagaat tgtatttgaa gaattactta
    27121 tactacaact agggttgttt gtatttaaaa gtggcagaaa taaagaagat ggaataaagt
    27181 ttgaaacaag taaagactta aaaaaaataa taagtgcttt accatttaaa ctgactaaag
    27241 ctcaaaacag ggcattagat gaaattatac aagatatgaa tttagagaaa ataatgaata
    27301 gattggtaca aggtgatgta gggagtggta aaacagtagt agctctctta gcacttgcaa
    27361 attgtgtttt aaatggttat cagggcgctt tgatggcacc tacagagata ttagcagggc
    27421 aacattacat ttcacttact gagtctctaa aagattttgg aataaatgta ggtctattaa
    27481 taggtagtct tacaaaaaaa caaaaggata cagtattgga acaaataagg aataatgaga
    27541 ttgatatatt aataggtaca catgcactaa ttgaagataa ggttgaattt aataatattg
    27601 gtttagttat aacagatgaa cagcatagat ttggtgttat gcaaaggagc aaactttcat
    27661 tgaaaggagc aaatcctgat atattagtaa tgacagcaac accaatacca agaactttag
    27721 cacttatact ctatggagat ttagatattt ctattataga tgaacttcct ccaggaagac
    27781 agccaataga aacaattgct attgaaaaaa gtaagcgaga tagagcatat aataaccttg
    27841 taagaagaga ggttgaatca ggaagacaag tgtacattgt atgtcctttg gtagaagaaa
    27901 gtgaagctat agaagctaaa tcagcagttg aacttgtaga ggaattaaga gctgaatatt
    27961 tccacgattt aaggcttgga ttgcttcatg gtaaaatgaa atcgagtgaa aaagacgaag
    28021 taatggaacg ttttaaaaat aaagaaatag atattttagt ttcaacaaca gttatagaag
    28081 ttggtgtcaa tgtcccaaat gcaacattaa tgataataga aaatgcagaa agatttggac
    28141 ttgcacaatt acatcagctt agagggcgag ttggaagagg tagtcataaa tcgtattgtg
    28201 tactaattta tgattctaaa acagatgtat gtagacaaag aatggctata atggaagaga
    28261 ccaatgatgg ttttaagatt tcagagaagg atttagaaat aagaggacct ggagaatttt
    28321 ttgggacaag gcaacatggt cttccagaac ttaaagtagc aaacttattt aaacatataa
    28381 aaatattgaa gttagcacaa caagaagctc gatatatatt gggtgaagat aataatctac
    28441 aattgaaaga aaacatggca ctcaaaaaag aaattataga taaatttaaa gatacattga
    28501 aagaaatttc attaaattaa ttttaaatta tgttaaaata ttactaatgt aataatgtgc
    28561 tgaagggaga ttgtcatatt gagagtaatt tcaggaaaag cgagaggatt aaaactaaat
    28621 actccaaaaa atgaagatgt aaggccaact acagatagag taaaagagtc tctatttaat
    28681 atgataaatt catatataat ggaaagtgaa gttctggatt tatttgctgg gactggttct
    28741 ttaggaatag agtgcttatc taggggtgcc aagtcatgta cttttgtaga tatcagtaag
    28801 gaaagcatag atattgttaa gtccaatgta aaaaaggcaa gagttgaaag tgaaagtatt
    28861 attttaaacc ttgattttaa gactgcaata gataaactta aattacaaaa tagtaaattt
    28921 gacataattt ttatggaccc tccgtattat aaaaatatgt ttatagaagc tattgaaaaa
    28981 attgataatt ctaatctatt gaacgaagat ggaataatag ttgtagaaca tgatacgaat
    29041 gatttatttc cagataagat atgtaaattg gagaaaacta gagataagaa atatggaaat
    29101 actacattaa ctttttataa gatggaggca taatatggaa aataaaccta gaaaagctat
    29161 atttgcagga agttttgacc caattactaa tggacattta gatattattt gcagggcttc
    29221 gaagttattt gacgaattac aaataggagt attaaataat cctaataaaa agggtctatt
    29281 tagctttgat gaaagagtaa aacttataga aaaaagtaca agccatttaa ataatataaa
    29341 agttgtgact tttgatggtt tattaataaa ttactgtcag gaaaatggga ttggagcatt
    29401 agttagaggt gtgagaagtg gtgctgatgt tgattatgaa cttcagatgg cacatatgaa
    29461 tagagagctt aatccagata tagagactat aattttacca agttgtacaa aatattcatt
    29521 tataagttca tctttaataa aagaggtttt gctctttgat gctgatataa aaaatttggt
    29581 tccaaagatt gttttggaag aattaaagaa aaaaactagt gggggtaatt gatatatgaa
    29641 gatagattta gaaatgattg aactatttga gcagttagag gagatattta gaagtgcttc
    29701 tacaatccct ttctcacata aatgcacagt ggataaagat gaggttttag caataattaa
    29761 tgatataaga actttaatac cagaagaagt aacacaggca gtatggatta ataaagaaag
    29821 aaataaaata ataagtcaag ctaagcaaga tgctacaaat atagttgaac aagcgaaaaa
    29881 agaagctgag agaatacaac aagaatatca agataatata gaggaattaa agaaaaattc
    29941 tgaagatgta gtaaaggcat atgtagaatc cagcgaacct gttgttcaag cagaccaaag
    30001 agcaaaagat atagttgata gagctgagag aatagccaat gagataagaa ttgggtcaat
    30061 tgaatatgca gaggatgtgc tttctagtgt tgagtataat ttaagagaaa tattagaaga
    30121 agtaaaaaga gataaagctg aacttagacc taaaaaataa attttaaata aataaaaatg
    30181 gagctatcac acaaaaaatt ccaattgatg tgtgatagct ctcttttaat atagcaattt
    30241 tttgttatat aattgtgctt tttagattac ttatatctta ttttgatatt ataatgtagt
    30301 ttaatctttt atttgttgaa atcaacttat ttatttgtat aaataatata ggttattttc
    30361 aaattttttt aataatttta ccactgtctt cttttagatt agggttagac tgtgcaaaat
    30421 tataaaataa tactaaatca tgtgcaatta tttggtatgg cactacatgt agaaaatata
    30481 gataataaaa ttaaggaggt agtatgaagg aaagggctaa aaaaatatta gattttatta
    30541 ttaaaaataa caatgtaaca tcacaagaac tacaagaaag gtttaatgtt tcaaagagaa
    30601 ctatttatta cgatattcta gctataaatg aacaactagg aaaaagtggt aatataaaaa
    30661 atgtaaaaca caaatttata tttgaaggta atttatgtga tgcaaggaaa ataatttcaa
    30721 ctgaagaaga taaattttta gatagtgatt acagaaaaac ttttatatta aacaagatat
    30781 tacttggcga aaaaatatca atagaaaaac taacaaatga aatgttactt tcaaaaaaca
    30841 cagtagtaca aacaataact gatgtaaaaa aataccttca aacaatgggg ttaagattag
    30901 agtataaggg aaagtacaaa attataggtg atgaatacgt tattagagag ctgtttctga
    30961 taattgttca agaaaatgta ctggaaatta attctataag taaggaagta agtagtttcg
    31021 atacaaaagg acatataaaa cttactgatt attctttatt aaatctaact aagtttgtgg
    31081 aatttctaaa taaaaggata agagatggga aaacattata ctcatataaa tatctaaatg
    31141 aagctaaaaa aattagttac tttagtaatt gtaaagaact tttatgtgaa gaagccaatg
    31201 aaaatgaaca agcctatata tgcacttaca taagttcact tcctagctta aatagtgaag
    31261 ttaaggaaga tgttgttgaa gaatatgtag ataaacttat agataagttt gaagtaaata
    31321 cagcaataaa attagaaagt aagcatgagt ttaagaaaaa tattttaagg cacctccact
    31381 cttcttacaa cagaatacga tttaagtttc ctattagaaa tccaatgtta gatgagacta
    31441 agtataaaca tgagtcactt tataaaatta taaaatcaat tatagaaaat gaagaagagt
    31501 ttcctgtatt tgaggggata agagaagaag agataggatt tatagcagcg tattttggag
    31561 ggtatttaag agggtctagg gataatggcc taagaagaaa taaagtctta ctagtttgtc
    31621 ctaatggtct tatggtatca aagagtttgg aaatacaact atataaatac ataccaacca
    31681 tagaaatagt aggtatagtg tccattaagc aactaaaaga agtaaatgta tactacgatt
    31741 acataattac aactatagat atacaaaatg taaacaatgt aattgttgta aatcctcttt
    31801 taacatcatc agatgttcaa ttacttatga acaaactaat aagtgtaaaa gaaaacgaga
    31861 agtatttcaa tcttgaatta ataatacaag ctataagaaa aaatggagtt atcaataatg
    31921 aagaagcact aaaagctgat ttattaaaca taatacacaa gatagatgaa ggagagatgt
    31981 atcaaccaat gttaaaggaa ttaattaatg cagaaagggt caatataata aaaagtgtta
    32041 gagattggaa agaagcaata aaaatagcct caaagccatt gttagaagat aactctatag
    32101 aagagttata tatagaaaat atgataaagt cagttgaaaa atatggacca tatattgttt
    32161 tagctgatag atttgctcta ccacatgcaa gttctaaaga aggagtaaac aagcttgcta
    32221 tgtctttact aatagtagaa gatgaagttg atttattagg aaaaccagta aatatattta
    32281 tggtattagc tgctgtagat aacactactc acattagagc attagcatca ctatcagaga
    32341 tgatgtacga ggaagaaaat gtaaaactaa taattaatgg tgataaaagt tcaatactag
    32401 aacttataaa taagcaaaat taggaggaat gaaaaatgaa gatattagca gtatgtggtt
    32461 ttggtgtagg ttcatcaatg gtattaaaaa tgacaataca aaaggctcta aagcagttag
    32521 gtatagaatg tgaagtagaa aatacagata taaattcagc tagaggtgct gattgtgact
    32581 gtatattcac aagtcaagaa ttgttaaatg aattaaaagg aacttgtaat gtagatgtat
    32641 atccagttaa aaagtacatg gatttagcag aagtcacaga agttgttcaa acttttttaa
    32701 gtgataaaaa tgataagaaa tagtataagt tttaaaaagt aaatttaagt taatcagaat
    32761 aaaataaaaa cttactaaat tcaaaaatag taagaaagag aggagggatt tagtggtaga
    32821 aaacttaaac agttgaccta tcactaaaaa atataatgct tgattttatt tcaaataata
    32881 ttttacgtaa tccatctatg ttaattggat taatagcagc atttggtctt gtactacaga
    32941 aaaagaaagc atctgatgtt ataaaggggt cactacttgc agctttcgga attataatta
    33001 tggagactgg aacaggtatg ttagttagtt caatagcacc aattaatgga gctttccaaa
    33061 gtataagtgg tggtgcagta acaaaaggtt taagtgatgt tacttttact gcaagttatg
    33121 gtggaattat aggtttagca atgtttgcgg gtttagctat tcacctaatg atagcaagat
    33181 ttactcctat taagacaatc ttcttaacag gtcacatgct ttattggttc ccattcatat
    33241 ttgtagcttc aggagttgaa gctggattaa atggtggagt attaatagct tttgcagcag
    33301 tattatcagc actctattgg tcaataatgc cttggttatt aagaaaatat gtgtttgatg
    33361 ttataggaga tgattctttt acattaggtc atccatctgg ttgtttagca cttatatcag
    33421 gatttgttgc taaattagta ggaaataaag aaaaatctac agaagatata aacatacctc
    33481 aaagtttatc attttttaga gaaatatcta taagtggtgg aatggcagtg tttttaatgt
    33541 ttatagttgt tgggatagta ataccaggtg cgtttaaaga tagtaatgag tttattggaa
    33601 cgtatgcaat aaagcaaggt ctttcttttg gagcaggtct tataataatg cttcatgggg
    33661 taagaatgct aataaatcaa ataatgccag catttcaagg tatatctgaa aaagttgtgc
    33721 caaatgcact acctgctttt gactgtccaa tattatttaa ttataaacca aatgctgtat
    33781 taataggatt cttagttgct atgctaacat ctacagctat gattttaata tgtaactact
    33841 ttaatgtatt taatttgcta cttataccac ttgttataac ttcattcttt gagtgtggag
    33901 cagcagcagt aataggagaa tcacaaggtg gtatacgtgg agctataata ggtacagcta
    33961 tagctgcaat atttatggta gttttagtag gaatatctgc tgtaatttac tcaggaacta
    34021 ttcaaaattg gatactaata tttggaggta acgacctttc attatttggt ataattagta
    34081 agtttatagg ttcactattt ggaggaatat tatagatatg aaatttaaat acatagatga
    34141 gataagaaga ttaattgata taatagaaaa cgaagaaaaa gtcaatatgg aaaaagcagt
    34201 acaaatcctt gttgaagcaa tagaaagtaa aaatgctata ttttcatttg gagcttcaca
    34261 tgcaggaata ttaacagaag aattattcta tagagcaggt ggctgtgttc ttataaatcc
    34321 agtatttgca aaatctctaa tgttagatac agcgcctata actcacacaa gtaagatgga
    34381 aagattagaa ggatatggta cagctctagc tgataaaact ccaattaaaa aaggagatgt
    34441 agttatagtt cattcagtat ctggtagaaa tccagtaagt atagagtttg ctatgaaagc
    34501 taaggaaaat aaggcaatag taatatgtat aactaattta agttactcaa aaagtgtatc
    34561 ttcaagacat tcttcaggaa aaaaacttta tgaagtatgc gatttagtga tagataatca
    34621 tggtcaaaag ggagatgcct gtgtagagat agatggatta aaccaaaaag ttagtccaac
    34681 ttctactgta atagcaacta ctgtgatgaa ctctattata gctcaagcta ctcaggaatt
    34741 agttaataaa gggctaaaga atcctccgat tttctatagt gccaatattg atggtggaga
    34801 tgagttaaat aagaagattt ttgatgaata taaaagtgta attcattatg aatattaagg
    34861 ttaaaaaaat atgttaatga actttgaagt ttatcaacaa agaatattaa taatatataa
    34921 taaatgctat catttaaaag ctgtaggtaa aaatctacag ctttttatta ttgtagatta
    34981 aaaagatgcg taattaaatt ttaaattgga gaaaataaat tggaaggtat caattataaa
    35041 tattagaaac gttgtcatag aaaaaaacta tattaaaaaa aagtacatag aaaaaactga
    35101 ttagacaata aatacatgct ttgataatat tttaaatgga gtgaaaatgt gtagaattat
    35161 aatgaaaaaa gaaaataaaa atgaaaaaag aaaataaaat ggagggaata aaagtgggta
    35221 tgttaaaaaa attatgttca ctagctatgg tagcaattat gactacaaca cttataacag
    35281 ggtgttcaag tggaaatgga aaagattcaa aaaaggatgg cgaaaaagaa aaactagttc
    35341 tttggatgcc accagtagaa aataatatga aggaagtttg ggaccctatt ttagataaat
    35401 ttgaggaaaa aaataactgt gaagtagatt tacagataat tccttgggat aattattctg
    35461 aaaaatttgc aacagctata agtgctggcg aaggtccaga tgtaggatat atgtatgcag
    35521 aaatgtatcc acagtttata gaaagtggag cagtagaaga tttgactaac tatgcaacta
    35581 aagaggataa agagcaatct atttatataa aaacatctga aatgatgggt ggaatgtatg
    35641 gtatgccatt ccaagcagca aatccagggg tattatacta taacaaggat attttagaga
    35701 aattaggaga gaaacctcct aagacatggg aagattttaa aagaatttgt aaaaaagcaa
    35761 cacaagatac tgatggtgat ggaaagatag accaatgggg attagctcag ggatggggag
    35821 ctaaaacttt tggtaactta aactggaact ggtatcctta tttatggcaa gctggtggag
    35881 atatttttaa tgatgattta aaatcggtta aatttaatga taagagtggt ttagaagcag
    35941 ctaatttctt aaaagaatta caagcatatg tgcctgaaga ttctttatct aaagatagta
    36001 atgaaatgat agaaagcgta tttggaccag ggaaagcagc atttacaata atgttatctt
    36061 ctgctgcaac aagtgtattt gatgaatcat tcccggattt aaattggggg tttgtaactg
    36121 gactagaaga taagaaagca gcaacttttg gagcagtaga ccatttatct ttaatgtcat
    36181 ctgctaaaaa taaagaactt gcatacaagt taatacaaca tatgttaagt gttgaatcaa
    36241 tgacagaatt tcataaagca ataccaagag caccaataac aaaaggtgaa ccatatcaag
    36301 gtgatgaaag attcaaagaa atggtggaaa atgacaaaaa tgtatataga ccattagtag
    36361 taggacctca tggagttgaa atatatgaat atttatggaa agaacttcaa actatgattt
    36421 caggagataa aactccaaaa caagcattag atgatgctgc aaaatattca aatgatttat
    36481 tagcacaata aaagtttaaa gctgtaatat gttgtaaagg actttacaga ttaaaattag
    36541 cattgtattt ataaatacaa tgctaatatt tatattggag aggataaaat ggaaaactta
    36601 gttagagagt ttgaagaaaa ttcaaaaaga aaaaaaacaa aattaaatat taaaccatat
    36661 atgtatatat tgccattggg aataatttta gtggcgtttt atgtgatacc aataataatg
    36721 tcaatatact ttagttttac aaagtataat attattagtc cagccacttt tataggttta
    36781 gaaaattata aaaaattatt tacagatgaa acattaaaag tatctattat aaatactatt
    36841 aaatttactg ttgttgtagt accttgccaa actatattat cattgatttt ggcagtttgg
    36901 attacaggaa aaggaaatag taaaatagct tcatttgcta aaggtgctat atttatacca
    36961 gtattatcat ctatggtttt gataggtatg gtatggaggg ctttattgaa tggagaaggt
    37021 tctattatat atcaggtatt aggtgccttt ggaatagaat catctaaact tttaggagat
    37081 agtaagacag cattacctac acttatgttt atatctatgt ggaaaaatat agggtatttc
    37141 atggtaattt atatttcagc tataatgaat ttaccaaaac actgctatga agtagcgaag
    37201 gttgatgggg ctacaaaact tcaagaattt ataaaaataa ctgtaccttt attgaaacca
    37261 actacaataa tggttgtatt tttagggtcg atatggtcat tacaagtatt tgacttaatt
    37321 tatactgtga caggtggagg tccaggtata tctactatga gtatagttat gcatgcattt
    37381 aacttaaact ttaaaaactt taattctgga tatgcaatga ctgtagcaaa cgtattattt
    37441 ttgttaatag cagtagtttc gattttacaa aataagcttg taaaacgaga caattcagat
    37501 ttttagaaag gtaggtataa tatgaaaaca gcaagaagat tattaagtga tggtttactt
    37561 ttatttatag cgtgtgcaag tttggtacct tttatataca tgttgataat atcattgaag
    37621 ataacatata actcatatag tttggatata tctttttcaa cagtaactct tcaaaactat
    37681 atagatatct ttactaaaaa aggatttgca caatattttt ttaatacagc aatagtatct
    37741 ttttcaggtg tattattaaa tttagtattt agtacattag caggatattc ttttgcgaag
    37801 atggatttta aaggtagtga taagttattt ttatttatga taatgacttt aattatacct
    37861 tcccaagtta caatgatacc actatatata ataatgaaac acttaggatg gataaatagt
    37921 tatcttgcac ttattatgcc aataccaact gcatttggcg tatttataat gagacaagct
    37981 attttaggag taccaaaaga gttattagaa tcagcaaaga tagatggttg ctcggatttt
    38041 agaattttaa tacaaatagt attgccttta atcaaaccag cattaattac actagcaata
    38101 tttactttta tgggagcatg gaacgagttt atgtggcctc ttatagctac aactaaagat
    38161 gcaatgagaa cattaacagt tggattatct actcttaaga cattccagat aactaattat
    38221 ggacagatga tggcaggggc tacaataaca tttttacctc catttatttt ctatttgatt
    38281 ttacaaagta aatttgttga aggtgtgtct ttgtctggta taaaaggtta aatagtctaa
    38341 tttaataaca taaaagaaac ttatataaat atggaggaat ttaaactatg gaaatttcat
    38401 atgtactgaa agattgcaca ttcaaacaca ataaatactc agttatagaa gctgaaactt
    38461 ggaaagagaa ggatttggat attgtagtag ctaaaggtga aaaatttgca tttcaaatta
    38521 tgctaaaagc aacagaagaa tttaattgta caattgataa aagcagtact atatcatgga
    38581 aaggactgga aaatagagta agattagcat taaatgtgcc taatagttta gaaaataatt
    38641 ttagtataaa tatattagga tatgttcaag atgatacaaa ggcatttgtg aatgatgcta
    38701 ttttaaggga taaagatgtg ttagtagagc aatatttgcc tcaaacattt tggattgaag
    38761 gtcaagtacc agaagatttt gaagaaaata accttaatat aggtattgat atatttaaaa
    38821 gctttggata tgaagatgaa gaaaaagttt gtacaattga tgttcctgta aaagtaagaa
    38881 atgtcgtgct aaagccttta gataaaagca agttcttttt agatttatgg caacatccaa
    38941 gttgtttagc tagaatgtat aaagttgagt tatggtctga tatacatttt gaaatagtcg
    39001 ataactattt aaaagaatta gcatctttag gagaaaaagt tgcaacagtt atagtttcag
    39061 attatccatg ggcagggcaa agttgttata aagtttataa aaatccatca aacctatatg
    39121 aatataacat ggtaagtgta tcaaaaggat tggatggaaa gattaaatgt aactttgaga
    39181 gtatggatag gtatataagc atagctgata agtataaaat gtctaaggag atagatttat
    39241 ttggtctatt aggtaattgg tgtgctggtg agtttggaaa tcctgtagaa ggttataagg
    39301 accctataag agtaagatat tttgatgaaa atgataaagt atttaaattt ataaacaata
    39361 caaatgattt aaaagaatat ataggattag tattaaatca tttaatagag tgtggattat
    39421 gggatagagt aaggattata gctgatgaac caaataatcc agaagtagtc aaagagtgta
    39481 tagaatttat aaattctact gtaggtactc atcaagttaa atataaatca gcaactcacg
    39541 accaaaactt tttagataga gctaaagatg aaattgatga tatgtcaata aatttaaaac
    39601 ttactataca aaattataaa gatatagaaa atcttaagaa aaaaatcaat gacaagggtg
    39661 ggatattgac ttggtttgta tgctgttttc cagagaaacc aaatagtttt ttaagttctc
    39721 cttttgtaga aaatagaata ataggatggt atacttatta ctttggtctt gatggatttt
    39781 taagatggga ctataattta tggacagaag acccttggaa agattcaagt tacaaattcc
    39841 ctatttggaa agctggagat atgttctttg tttatcctgg aaaagattta aagccagtta
    39901 gaagtgtcag aatggagaat ttaagatttg gaatacagga ctttgagtta tttacaatgt
    39961 tagaaaaaga aaagggcaga gagtatatag aagttgagct tatgcaagaa ttattaaata
    40021 aaaaagaaaa tgctgaaatt aaaggttttg gtgacataga attaggatat tcacttgata
    40081 attggggata tgataaagtt aaaaaatatg tattagagtt gttagacaga gtataaaaaa
    40141 atataagagt gtctcaaaat gaacttttta gttcatgagg cactcttttt gtatgaaatc
    40201 aatatagttt cattgtttca acaatgaaaa ataggattac ttatattcta agatagccct
    40261 ttagtttttt agttgtattc aatgttttaa aactaactat aaacgctata aagtttataa
    40321 atgacaataa ttatatgtaa catgatgtac tttaatttgc tataataggg attgaataat
    40381 attatatgga agataacatt gtttgggggg attaatataa aaagaatagg tattattata
    40441 atgttttttt taagtatagc ttttattgga ttttatcttg caataagagg taacagtgat
    40501 acagttaaga tgcaacgagg gataaatata ggtaatgcac tagaatcacc taaggatttt
    40561 ccgtgggatg taaaaatgtc aaataagttt tttgatgata taaaagatgc aggttttgac
    40621 acagtacgta taccagtaag gttttctgat tatacgtcag attctgataa ttttaaaatt
    40681 gatgaagaat tttttaaaaa gattgataaa tatgtagatt atgcattgga taaagactta
    40741 atagttgtgt tggatttaca tcattttgaa gaaattatga aagaaccaag agttcataaa
    40801 gaagagtttt taaaaatatg gcaacagata gctaataggt atcaaaaata tgataaaaaa
    40861 ttggtatttg aattattaaa tgaacctaaa gaaaatttat cttcacaatt actaaacgaa
    40921 tacattgaag aagcaataaa gataattaga aaaactaacc ctaaaagaac tattatagtg
    40981 ggaccatata atttctatca aattgattat ttgaatgaat taaatgttcc aaaagattct
    41041 aatatagtag tttcttttca ctattacgaa ccaaatgatt ttgcatttca aggaaacatt
    41101 tatcataatg gatttgagca cttgagcaat attacttggg aaggaacaaa tgaacaaatg
    41161 gattatttaa aaaaaaggtt tgatattgta gaaaattggg caaataaaaa taaggtaaaa
    41221 atattcttgg gtgaatttgg agtaacaaaa gaagcaccag aagcttcaag aagagcttgg
    41281 ataaaagctg taagagaaga agctgaaaaa cgaaactttt catgggctta ttgggagctt
    41341 gcttctggat ttggtatata caatcaaatt gaagggactt gggatagaga catattaagt
    41401 gcattaatag aaaagaggtg attttatgaa gaaaaattca aaaaagatac ttataacttt
    41461 aactattatt actaatatcg tatatatatt gtggagaatt ttttacacag tacctaaaga
    41521 agaagggatg tttgccttaa tctgtgctat aatacttctt tttgtagaga taatgggtat
    41581 gatggaaatg tttgtacatt attatggtat gtcaaatatt gagtatcctg aaaaaccagt
    41641 cataagtgaa gaactttatc cacatgtaga tgtattcatt gcaacatata atgagtctgt
    41701 tgatttggtg agaaaaactg taaatggatg tattcatatg cagtatccag ataagaaaaa
    41761 ggtacatata tacatatgtg atgatggaaa tcgtgaagaa atgcgtattc tagcagaaaa
    41821 aatgggtgta aattacataa ctagaactga aagagaagga gcaaaagctg ggaatttaaa
    41881 taatgcaatg caacatacaa actctccttt gattgctacg tttgatgcag atatgatacc
    41941 aatgcatgac tttttaattg ctacagtacc atactttttg aaaaatgagc aagctaaaaa
    42001 agatggagaa aaagaggagt atgaaaaggt tggttttgtg caaactccac agagttttta
    42061 taatccagac ttgttccaat ttaatctgca ctctgaggga aggattccaa atgagcaaga
    42121 ttatttttac agagatattc aattggctag aaataggaca aattctgtaa tttatggtgg
    42181 tagtaataca gttatttcaa gggaagcact tgaagaagtt gatggatttt atacttattc
    42241 tattacagaa gattttgcaa caggtatctt aattcaaagt aaggggtata gatgttatgc
    42301 tattccagaa gtacatgcat caggtttatc tcctacagat ttaaaaagtc ttattaagca
    42361 acgtgaaaga tgggcacgtg gatgtataca aactggtaga cgtctaaata ttttgtttag
    42421 gagaggttta ggtttttggc agaaaataag ttatatttct tcaattactt attggtatgc
    42481 aagtataaaa cgctttgtat atataatggc acctatttta ttttctgtgt ttaatgtgat
    42541 tgtagtaaaa tgtacattac ttcaagtatt ggtattttgg ttgccaatgt atattttaag
    42601 tagtttatct cttaaaatat tttctcaaaa tataagaaat actagatgga ccaatatata
    42661 tgaaactata atgtttcagt ctttaatgcc agcagtaata cttgaaacgt ttgcaatatc
    42721 taaaaataaa ttttcagtaa caaataagaa taaattagaa gaaaatagaa tgtataagtt
    42781 tttacaggga ataccttatt ttatttacat ggtcttatct atcataggaa ttttaaaaat
    42841 gtttgtagca atatttaaaa tgagttcaat gacatattca gttgtattat tttggttgat
    42901 tggaaacctt tttaaccttg ttatggctac attatttata tcagggagac aacagttaag
    42961 aaagtctgag cgttatattg ctgaaattga ttttaagtta aagcaaaact cttatgttct
    43021 ttcttcaaaa acgattgata tatcagaaaa tgggtttgca tttttgctag aaaatccaga
    43081 atatatttcc ccagaagaag agttcgaggt agagtttaga gaaaaatcag gaaatgaaat
    43141 gtatatagct aatatgaaag caaagattgt taatgttgtg gaagtaaatt caaagtggaa
    43201 atatgcagct tatattacac atattgaaga tagtgaaata gataattgga tgtgtattgt
    43261 tcacgataga atacctactt taccgatgac aatatctaat caactaggtt tctttgatga
    43321 tttgcaaatt aatgtaaaaa aacgtattga aaagattaga acattatcac gaagaagtcc
    43381 aagaatcaat atgaattttc agatggatat aaaaaataca ggtaaattaa gaatagtaaa
    43441 ttttaattat caatatgtat tattaaattt tgagaataaa aatatttatc ctaaagaaat
    43501 agcattggaa attaatgaaa atattgtgtt agaatgtgat ttatgtgagg gtaaaatcga
    43561 tgaaagaggt ctcttatata aagtaaataa cattgacagc ataatgcaaa atttcttttt
    43621 aagagatgaa atgatggatt ggatattaca aaataagaca attcctgttt caaatcctag
    43681 tgaaaagaag gaaaaaagta tagatgagtt tgaacccatg gaatatattt aggagagatg
    43741 aaatgaagac tttaaaagtg tttttttgtt ctgcaaaata tcttattttg gtgtttattt
    43801 tttttataaa tactactttt ataagttcag cgacaagtga taaaatttta gatttatcct
    43861 ttaaaaagat agagactgat ttaagttcta ggattacata tgaagataca ggtgtaaaaa
    43921 tagaaacaga tagttctaaa agtgataagg aacgttattt atatatttac caaaatataa
    43981 aagaaaattg gagtatgtat aataattttt atattgaaat tcaaaataaa aataaaagtt
    44041 cccagaaaat aaatttaagt atacaaagta agaatatgct tgaatttagg ttaaaagaag
    44101 gtagtgaaat atttttggaa gataaaaata taatatattc agataaaata aaagaaggat
    44161 gcatagaagt accaggtgaa tttgaaggca aaatttatgt gaactttaat agccttataa
    44221 atgagaaaag taatgttgtg ctggactcaa gtatgctttc aaacattgtt agttggggaa
    44281 ttacatttat tccatctgat gaagaacata atattgttat tataaaaaag atatctttat
    44341 tgtctgaaga gaaattaagt tttttacata atattaaaat aactggcaat gaagaagtac
    44401 aaataccagt attgggtcaa agtataagtc agtatgaggt attaggtctt aaacgtgatt
    44461 ctaaaattaa gtattctctt atgggaaaac aagataatgt gagcatatct caaaatggta
    44521 aattaacatt aagtaataaa gccgaacctg gacaaattat tttacaagtt aatgttgatg
    44581 ataagtttaa aattggaagg aaaatagcat taactgaatc ttggagtatt aataaaaaag
    44641 ataaggatgg tgttccatat acattggtat ctcctgagca atcaccaaca gtacaatata
    44701 tgaaaaaaat aaactttatg aataacatca taacgtttgt tcgaatttta tttgtatctc
    44761 tagtaataat atgctttgga atatatttat attggaaaaa atgttcaaaa actaagtaaa
    44821 gagaggaggt tagattgtgt tattttcaag tttaattttt ttattttatt ttttacctat
    44881 aactttggtt ttatattatg tatttaggtt taaccgaacg atacagaaca tgatattgct
    44941 tgctgttagt ttattttttt atgcttgggg agagccaaaa tttgttgtta tcatgatagt
    45001 ttccataata atgaactata tatttggttt actagtagat agatataggg aaagtaaaat
    45061 aaaagtaaaa gtttttttag ttttgatgtg tgtatataat ataggtgttt tatttatatt
    45121 taaatatcta gcttttgcgc taagaaatat tagtgaactt attaatactg aactaactat
    45181 acctagtatt gttttaccta taggaatttc attttttaca tttcagggaa tgtcttatgt
    45241 tatagatgta tatcgtagac atggagaagt ccaaaagaat cctttttatg tgggactgta
    45301 tatagcattt ttcccacaat taatagcagg tcctatagtt agatatgaga gtgtggcaga
    45361 acaaattctc aatagaaaag aaacttggaa taaattttct attgggactt gcaggtttat
    45421 aacaggtcta ggcaaaaaag tattaatttc aaataacatg gctatagtag cagactatat
    45481 ttacactatg aatagtcaag gagaaatagc tgcatcactt gcatggcttg gttcaatagc
    45541 ctatactatg caaatattct ttgatttttc tgcttattca gatatggcta taggtttagg
    45601 tcttatgttt ggttttaaat ttgaagagaa ctttaattat ccatatatat caaaatcaat
    45661 tagtgagttt tggagaagat ggcacatttc tttgggaatg tggtttaaga gctatatata
    45721 ctttccgctt ggaggttcta gagtagcaaa taaagatatt atgattagaa atatgtttat
    45781 agtgtggtta tttactggaa tatggcatgg agcagaatgg acatttgtaa tttgggggat
    45841 tttaaacttt gttttcttga ttatagagcg atttataatg tttgaaaaga ttgaaaatca
    45901 caattttata aaacatattt atgcgttatt agtagttaac tttgggtggg tattgtttag
    45961 gtcacctaat ttaaaagaag catataatta tttcaaagct atgcttggag gcaatggagt
    46021 tatatggagt gattatacat atatgttttt aaaagaatac tgggttttct ttatatttgc
    46081 atttattttt agtgtaccta ttgcaaagaa aatcaataag tttgttgttg aacaagctaa
    46141 atatagtatg atatttaacg tattttatcc aatctcagct attttattgt tctttatttc
    46201 tgtgacatat ttagtaactg gaagctataa tccatttatt tattttaatt tttagttggg
    46261 aggaaaagta tgaatagatt ttttaaagta agattaataa ctgcaattat gttcttaatt
    46321 attttattcg ctttctctat ctttaatatt tgttattctg gaaaagatat gatacaggag
    46381 ataaaaaatt cagttgctga aaaaagtagt ttaaaagata caatatcatc tattgataac
    46441 acagtaaatg acaaagtaat atataaaaat acgttcattg aaacttatgg ctacttacaa
    46501 aagttaatgg gtaaaaatga gttttcaaaa tttagtgttg taaaagatac ttctggaaag
    46561 atgcattata catattttgc taaagctcca aatccagttg gtgtgatagc agatagggta
    46621 agaaaatttt ctgaggaaat ggaaaagcaa aacactaaat tagtttactt aatgactcca
    46681 gataagtata taaaaggagt gacaaaattt ccaaaaggaa taccttattc ttatagcaat
    46741 gagacagcag ataatttttt aaatcaacta aaaaagtata atgtggacta tattgatttt
    46801 agagaaaata ttttaaagag tggtattcca aaagatgatt tattttttaa aacagaccat
    46861 cactggaaag tagaaacttc tttttgggca tttggagaat tagttgagca acttaataaa
    46921 aaatacaata tgaatttaga tgaaaatcat tactatagag ataaagaaaa ttataactca
    46981 attgtatatc ctaagtcatt tcttggttct atgggaagaa agactggtat cttatatggt
    47041 ggtatagatg attttacatt aatttatcct aaatttaaga ctaattatac ttattataca
    47101 gattctaaat ctcaaaaatt tgaattaaaa ggaaggtttg aagagagttt aatattatca
    47161 tatcctttta atgcagacct tgatttaatg gatggtcaat cagataagta ttttacatat
    47221 ttattgggga atagaccatt agttaaaata aaaaatatag aaaatccaga tggattaaaa
    47281 gtgctgtttg ttaaagattc tctaattgta ccaacagcag catttttttc ctctgtttgt
    47341 tcatctatag atatgataga ccctagatat tatgatggag atatactaga atatgcaaaa
    47401 tcccatgact atgactttgt ttttatatct gtatatcccc aaaatcttac aaaagaattt
    47461 tttccgtttt gtaagtaaat ttctaaagaa attttcttat aaaataaaaa ataaatggct
    47521 aactagttaa aagttagcca tttatcttaa gtgttactct aagaaatgtt ttttttacca
    47581 aatacttctt tatctatttt tccaagtgat tttcctacag taattgatga taccatagca
    47641 ccattaacat ttatcatggt acgtcccata tctagtatag ggtctattgc aagtatacca
    47701 ccaactaatg gaaaataaga accaagacca actccagaga taacaactga aacggccatt
    47761 gtagcagttc ctggaagacc agctattcca aacgaactaa ttgttataac aattaacaac
    47821 ataacataaa ggctcatatc catgtttact ccagccatat tggcaagtgt tacagccatt
    47881 aaagcaggat atatacctgc acaaccattc atacccatat ttgcacctaa acttgctgta
    47941 aagcttgata caccctcatc aactccgtga ttttgtacta gactttctat cgttacaggc
    48001 aatgttccta aactagaacg tgaagtaaat gccagaagta aagcttcagt tgaatttctt
    48061 atataagtaa tgggatttag accatttaaa gttataaaaa ttaaatgtat ggtaaataat
    48121 attgccactg atatataaag agctaatata aactgtaaaa cagatactat tgagctaata
    48181 ccttgtgaag ttattgtatt tgcaagcaga gcaaccacag cataaggcat aaatttaata
    48241 acagtcatag acacacttac aattattttg taagatgctt ctataaaatc tataaatggt
    48301 tttattgttt caaagtgttt tttacttaat ctctttattg aggtagctat aaatgtagca
    48361 aatattattg tagctacaat atttccattt gccatagcca ttacaggatt agatggtagt
    48421 aatcctctta aagtatccac aagagagttt atttctttaa gttcttgatt ttcaactaaa
    48481 gtaggcatat tagctcctac acctaacttc attatatttg caacgataat tccaacaatt
    48541 gcagctatag cagtagtagc aagaagcata cctaaagatt taaatgtcag agttccaaga
    48601 ttgttatctt tcatatttat aattactttt ataatggata agaaaactaa aggtactact
    48661 aacattttta ataagtccat aaatccagaa ccaaataggc tataccactt gcttacttct
    48721 tgtagccaag tgacttgagt ggtatcatta ggaaaaccag caaaagcttg tattatgatt
    48781 cccaatatga gtcctagtac tgtagcatat attgttctca atgaaaattt tatttttttc
    48841 ttttcaaatt gtctaacaat aaagaatgta cctagaagta atattatgaa aaaaatcgtt
    48901 ttaaaatctg taatcataag aaattgtgat aaaaatgaac tttccaatat tttttctcct
    48961 tttatttaaa aataaatttt tctaagttta aatgatacca atatattaaa aaaatgtcaa
    49021 tattatatca caatttactt aaatagagta aaataaattg attttgaaaa atgtgattaa
    49081 gtatagaaaa atagggtata ataaatgttt aaagaatttg aattgtggta agaaaataaa
    49141 aaatagcttt tataatattt gatttcaaat taaaatatgt taaataaata actaaaaatt
    49201 gttatgaaaa atgtttacat agaatatata tcaaatgaaa agcttaaaat aagtgaagaa
    49261 attaactaga catcaaagga gagataaaat gagagtagta ataattggtg gtgttgcagc
    49321 tggtatgagt gctgcagcaa aattaaaaag aataaaacct gagtatgaag ttgttgttta
    49381 tgaaaaaact gaaatagtat cttttggtgc ttgtggatta ccatactttg ttggtggttt
    49441 ttttgatgat gctgatgaat tattagcaag aacaccagag aaacttagag aagctggaat
    49501 tgatttaaat atatttagag aagtcgttga ggttgatagt gaatctaaga aaataaaagt
    49561 aaaaaatata aaaacagatg aaatatatga agattactat gataaattga tgatagcaac
    49621 tggagcaaga agtattatgc ctcctataaa aaacataaaa ttgaagaatg tttctacatt
    49681 aaaaagttta tatgatggag aatatctaaa aaaactttta agtaatgaag ataataaaag
    49741 agtgacaata ataggagctg gttttatagg gttagaagca gttgaggcat gtaagaaatt
    49801 aggtaaagat gtagatgtaa ttcagttaga agatagaata ttgccacaag tatttgataa
    49861 ggagatgacg gatgtattag aagaagaaat aaatagacat aatgttaatt tgcacttgga
    49921 tgaaatggtt gtagaacttt gtggagaaga taaagtagaa aaagttataa caaacaaagg
    49981 ggaaatagat acagatgtag taataattgc tactggtgtg agacctaata ccgagttttt
    50041 gtcaaatact aatataaaaa tgctaaaaaa tggagctata gtagttgatg aatatggaag
    50101 aacttctgta gaagatatat actctgctgg agattgtgca actataaaga atatagtaag
    50161 taatgaaaat gtatatgttc ctttagctac tggagctaat aaattaggta ggatagtagg
    50221 agaaaaccta gcaggaagag aagtatctta tcaaggttca ctatcttcaa gttgtataaa
    50281 aattatggat atggaggcag catcaactgg aattacagaa aagcaagcta aagatttagg
    50341 cataaatgtc aaatctaaat ttatttctga ttataatcaa actcattatt atccaggaag
    50401 aaataaaata tatgtaaaac ttatttatga tgcagataca aaagttattt taggcggaca
    50461 agtggctggg tttaaggatg ctgtccaaag agcaaatgta ttggcagctg ctatctttgg
    50521 aaaaatgacc acaagtcaac ttggaatgtt agatttatgt tatgccccac catttgcaag
    50581 aacatgggat gttttaaatg tagctggaaa tgtgtctaaa taatatttag attatagcta
    50641 atatagttga caagcaatta agggaatgaa tttacctaaa ctatttttat catggtaaaa
    50701 ttcatcccta aatttttatt ccaaattttt agaaataagg attccattaa attattcatc
    50761 aaaatttaaa cgcaagctta atctatcttc tccaagaata gtattttcaa aaacattagt
    50821 agcattttct aaatacatac taggtatgtc taaagaagga ctaaaatggg tcagtaaaag
    50881 ttgtttgaca tttccaagtt ttgcaagatt agcagcttct ctaaaagtca tatgtttgtt
    50941 tctaacagct tttgatatat ctaaatcatc accatacatg gcttcacata taaataaatc
    51001 actatctttt atgaaatctg gtattgattc tataggtctt gtatcggttg taagacttat
    51061 cttaactccc tttctcaatt ctcctaagac catatctgga gtgtattgtt ttgaatttaa
    51121 aactatattt tgaccttctt ggagtttgtt ccataatatt ttaggaactt cgtttttagt
    51181 tgccttatct atatcaaatt tagggtttct tctaaaatta aaactgtatc ctaaacactc
    51241 tatagagtgt tctaaactga ttgttgaaat ttctaaattt cttaaaactt tattatctaa
    51301 agaaaaattg ccttgagggt tttctataat ttttaaagtg tatggtacat attctactaa
    51361 atttctcata gaacgtatac aatcgactat tccaactgga ccaactatag ttaaatcact
    51421 tgtacgacca ctattcccaa ttgttgataa aagtcctaaa agtccaaata tatggtcacc
    51481 atgtaaatgg gttatacaaa tcaagtctat atctttaaag ccacattttt ttagcttcat
    51541 agatacctga gtaccttcac cacagtcaat tagtattttt ctacctttat agtttataaa
    51601 tacagaggat agaaatctgt ttggcatagg tacatttcca ccacatccaa gtaaagttaa
    51661 atctatcatt ttttcccctt tctaaaatta acacaaatct gttgtttgtt tttattatga
    51721 tatcctatta taattatttt aacattaaat ataacaataa atgtgtattt gagtcaagaa
    51781 atattatcaa ataaagtttt tatatagcaa tgtataaaaa tttggagaac gaaaagtgaa
    51841 aattatattt aggagtattt aaaggaggaa aatttaatga aagtattaaa gaaaattact
    51901 attttgtcat tgattacatt aaccttagtt ggttgtagta acggagaaga taaaaaagtg
    51961 gttgaaggtt ctaagaaaat tcaaaattct aaaaaagatg aacagcctat aatagtacat
    52021 aaagatgcaa atatattgaa agataggtat gagccaccaa aaggatttaa aagagttgat
    52081 gtagaaaaag gtagttttga atcatttctt aggaatacta agctaaaaaa ttatggagag
    52141 aaagtaaagt attatgatgg aagaattaag gaatcaaaag gtatttatga tagtgtattt
    52201 gatgttgata ttggcaatag agatttgcat caatgtgcag atgcgattat gcttatgaga
    52261 gcagaatact tgtatcagaa taaactatat gatgaaataa gttttgattt tgttgatggt
    52321 tttaaggcga agtattcaaa atgggcacaa ggatatagaa tttcagtaaa agatagtggt
    52381 tcaagctggt ataaagcaac tgaatattca acatcttatg agagtttcag aaagtttatg
    52441 gatatagtgt ttgcatattc aggaacgtta tctcttgaaa aagaattgga gcctgtaaaa
    52501 gttgaggata tgaaaatagg agatgtgttt atagttggtg gaagtccagg acatgcaagt
    52561 ataattatgg atatggcaga aaatgaaaag actggtaaaa aggtatttat gatagcccaa
    52621 agctatatgc ctgcacaaca aacgcaactt cttattaatc gtaaaaatcc aaatttgagt
    52681 ccttggtata gtttagattt tgaaggtgat ttgacaatac ctcaatggac ttttaaaaga
    52741 gaccagttga aaagatttta aaataataat tttatataat aaaagaaaaa tatagatgtg
    52801 aaaaaatgta attagattaa aactaattgg atatggacaa aattaaattg atagataaat
    52861 attaaaaatt acactaatca atcttactat agtttaatat ttatctaaaa agttttatgt
    52921 aaaatataaa ttttatatta aagctaaaac taatttgagg aggctaattt atattgaaga
    52981 ttagaaggta tttaattttg gtaattataa ttctaattac aacaagtcag agttttattt
    53041 cttatgggct agagcgattt gaagaaataa ccatttttca cactaatgat atacatggaa
    53101 gatatgctga aggtgatgac catatacaaa taggaaatct agccacattg aaaaaagaaa
    53161 ctccaaactc aattttagta gatgcaggag attgtttgca tggacttcca atagtaaata
    53221 tggatagagg gaaaagtgca attgaattaa ttaaatgtgc tggatatgac tatattactc
    53281 ctggaaatca tgactttaat tatggaaaag atagactgtt tgagttatct aaaagtgcta
    53341 atttaggaga gaatacttta aaatttttat catcaaatat ttttgaaaat ggaaaaaaag
    53401 tttttgatag taatgatata aaagaaatag atggaataaa aataggattt tttggactct
    53461 caacccagga aacaaagaac aaaactggat ttaaaaatgt ggaaggatta gagtttaaaa
    53521 atcctataga aagtgcaaca gaacaagtaa atgaattaaa aggaaaaggt gccaatgtaa
    53581 tagttgcaat atgtcatatt ggtacaaata gtttaagtaa gccaaatagt atagatatag
    53641 caaatgaggt tcaaggaatt gatttgatta ttgatggaca cagccacact aagtttgaaa
    53701 atggtaaaca agtgggtgat acattgattg taagtgcagg acaatattta gaaaatattg
    53761 gcgagataaa actaactttg gatactaatg atgtagaaaa tataaaaata gaaaataaat
    53821 cagttagatt aattggaaaa gaagaagcat taaagtataa gcttgactac gttgtaagta
    53881 aaaaggttga tgaaataaaa gaacaacaag aaaaaatatt agataaagtt ataggtgcaa
    53941 ctacaaatac tctagatggt tcatatgaaa atgtaaggac aaaagaaact aatttgggta
    54001 atttaataag tgacatttta ctagataaaa ctaaggctga tatttcttta tttaatggag
    54061 ggaatattcg agatacgata gaaaaaggca atataacaag aagagatatt gtagatgtat
    54121 ttccttttag caatactata gttaccaaag aacttacagg agcacagatt aaagatgtat
    54181 tggagcatgg agtaaaactt tatccagaaa aaagttcagc ctttttgcaa gtgggaggaa
    54241 tttcatacta ctttaatcct aaacaaaaag aaggcgaaag aataacaagt attcaaaaag
    54301 aaggaaagcc attagattta aataaaaagt atgtagttgc aacgaatgat tatatagcct
    54361 caggtggaga tgaatttcct tgtttttcaa aagagccaat actaaaggaa tttggaaatt
    54421 tagagagtgc agtcattgaa tatatagagt acaaaaaaga aatatcaaaa aatgtagatg
    54481 gaagaattgg tattaaatct aaagatgagt taataagcaa gaatcatgag agtgatttga
    54541 tattaagtga aaatgaaaaa aatatcaatg ataatgaaga gaatctatct aataatataa
    54601 aaagctctga tagtgacaaa aataaaagct tagataaaac aagtgctaga gaaagttcta
    54661 atcaaggaat atctaaaaga gaagctaaag aaaaatctcc taaaacagga gatttaggtt
    54721 tttcaaatag tataattata tttatagttt caagtacatt gatatgttta ttgaatttta
    54781 atcaaaaaga gttaaaggat aaaaagtcaa aataaaaatt aatgagcaaa attagtagat
    54841 agtaatttaa ttttagataa tagtaaacag caaaggaaat tagttatata aatactaatt
    54901 tcctttgttt tatgctttaa catgattatt tttaaataaa attgtatttt atgaaaaata
    54961 attacactaa atgg
//
LOCUS       DAELSY010000006        54749 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.006, whole genome shotgun sequence.
ACCESSION   DAELSY010000006 DAELSY010000000
VERSION     DAELSY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54749
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.006"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(120..929)
                     /locus_tag="KPG09_000307"
     CDS             complement(120..929)
                     /locus_tag="KPG09_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0964936.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTFVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(1013..1546)
                     /locus_tag="KPG09_000308"
     CDS             complement(1013..1546)
                     /locus_tag="KPG09_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG0964937.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGLNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(1673..3748)
                     /locus_tag="KPG09_000309"
     CDS             complement(1673..3748)
                     /locus_tag="KPG09_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0964938.1"
                     /translation="MLKGLSFRNARRQAGKYLIYWCSLIGSVALIYAFNALIFSDVMQ
                     ELSSILNNGGSDEFSYMIIIFSFIITFILGWFVSYMMNFILKKRSREISTYMILGVDK
                     KQIVKMLFIENLLLGASALIVGWILGIFVSKVLESIVINMFHGKYSLSPDFSLKAAGM
                     TFLYFCIIYLIAQLKSNKKLVKMKLINLLNYDRYNENVLIHQTKTKSIMFCVSVLCGG
                     MGIFFFSIPSGRLTDVFLGLLLIVFCLIVLFMGFGSIAQNMFEKGMNWKYKKSHLFLY
                     RLLASKINGISIIMGIIATLFTVSIACIGIANSFYSVMNKSVDLQQFDVSIFHPNESY
                     DFSSYEGYLSETKGVKKDYSYTLYTEEKTSFMKVRNNILSKYLKDIGRDLQYKDYTFA
                     ENQFDTFMKYSDYNILRNMLGLNPIKMDKNQFIIHCMPYLADAYKNYIGEKYKLTISD
                     KDLSIAGIYIENFSQYGGYGNGQEYIIVIPDYLIDNMKPLYSLYVAITSEPLDSAYFT
                     EFQKKFDTLENLNSGVVVAGNQGFVSKLRYDNKDYISGKYAMQSTGQAIILILPLFYL
                     ALIICIIGTVILSIQLLSENNIIIKHYGMLRTLGMIDSEVIKILKKHVCLYFSIPLIP
                     AVIFGGILVSVFSYNMFNISFDAPVFFNIQIPILHAVAITLINFVVIYCIYVFITYVA
                     LRKEILHIK"
     gene            complement(3751..4497)
                     /locus_tag="KPG09_000310"
     CDS             complement(3751..4497)
                     /locus_tag="KPG09_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0964939.1"
                     /translation="MLLRVENIEKYYGNQKILTKALNDVSFQINTGEFVSIMGTSGSG
                     KTTLLNCIATIDTVSAGKIYLENQDITELSEENLTVFRRDNLGFVFQDSNLLDTLTLE
                     ENIALPLTIKKVSPVEITNSVYNIATQLKIEEILSKFPYEVSGGQRQRCAVARAVVTE
                     PKLILADEPTGALDSNSSKTLMELLETINTILEATILLVTHDAISASYTERILFLRDG
                     KIFNELRKGEKSRLTFYHEILDALSLMGGD"
     gene            complement(4511..4906)
                     /locus_tag="KPG09_000311"
     CDS             complement(4511..4906)
                     /locus_tag="KPG09_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HBG0964940.1"
                     /translation="MKKRILTILCVVLVLFISITGLDSMGFFSRSIIPLRSEYASFIR
                     KNGTITEVKFTGPFNHNNIDLNKNVEDTNKNGDDNELTIKYDIEVKKSLISSNEPTIQ
                     IQDSKESIYVYILNFSDKTIIIKNGKIVD"
     gene            complement(5019..6041)
                     /locus_tag="KPG09_000312"
     CDS             complement(5019..6041)
                     /locus_tag="KPG09_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0964941.1"
                     /translation="MRFCNFLQDKVLVILLNICCAISLVLYLHLIGIGQGELTLLIAA
                     WVAILFCWICFCYQKQKKEIIKLRRIVDELDKKYLFIEMIEKPTSNIEREYYNIIRLS
                     LKSMTDHVSQIHREMYEYRQFIEQWVHEIKHPVTTSELICENYKNEHTRKIRFQMEEI
                     ERHIERALYYARLGHVEKDYLISEITLKSIVEEVLAKNKLLLIQNNIHINIQDLEYSV
                     YTDKKWIVFILNQIIINSVQYKSDSAEISFFAIKEDKSVKFTIKDNGIGIKESEIYRI
                     FELGFTGSNGRTTNNSTGIGLYLCYELSKKMGIDIDVSSSLGEYTAITLNFPINTAIN
                     MQKQCN"
     gene            complement(6038..6709)
                     /locus_tag="KPG09_000313"
     CDS             complement(6038..6709)
                     /locus_tag="KPG09_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0964942.1"
                     /translation="MILTNILIIEDDVFICKELMQFFENNGYSTQTLDTNNCDVLQVI
                     QESTPDLILLDINLPNIDGFSLCRQIRNCSTIPIIFITARDSAIDELNGLTMGGDDYI
                     TKPYNLPLLLARVKNLLKRVDSSDKQKIKYREIILNLISATVEYQNAVIELSRTELKI
                     MYYLFQNVGSIVPRIDLVEYLWDNHIHIDDNALSVNITRLREKLRGIGITDLIITKRG
                     MGYRI"
     gene            complement(6795..8144)
                     /locus_tag="KPG09_000314"
     CDS             complement(6795..8144)
                     /locus_tag="KPG09_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG0964943.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNRANSIEEIKPCEISKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIDKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEVIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(8156..9538)
                     /locus_tag="KPG09_000315"
     CDS             complement(8156..9538)
                     /locus_tag="KPG09_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG0964944.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            9782..10996
                     /locus_tag="KPG09_000316"
     CDS             9782..10996
                     /locus_tag="KPG09_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG0964945.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIKLYGNKCDYAKRDTLLYTANKLEIKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            11173..13086
                     /locus_tag="KPG09_000317"
     CDS             11173..13086
                     /locus_tag="KPG09_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0964946.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDNIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKVSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            13357..14331
                     /locus_tag="KPG09_000318"
     CDS             13357..14331
                     /locus_tag="KPG09_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG0964947.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            14352..14627
                     /locus_tag="KPG09_000319"
     CDS             14352..14627
                     /locus_tag="KPG09_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG0964948.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            14640..15803
                     /locus_tag="KPG09_000320"
     CDS             14640..15803
                     /locus_tag="KPG09_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG0964949.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGTEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            16140..18419
                     /locus_tag="KPG09_000321"
     CDS             16140..18419
                     /locus_tag="KPG09_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0964950.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVNFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSVVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPMPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(18514..19854)
                     /locus_tag="KPG09_000322"
     CDS             complement(18514..19854)
                     /locus_tag="KPG09_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0964951.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILIGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVTYYFIR
                     GKSTLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     IVQVFPEVFVGIFNKDSDLMDIAVRGISINLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(19866..20648)
                     /locus_tag="KPG09_000323"
     CDS             complement(19866..20648)
                     /locus_tag="KPG09_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0964952.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(20663..21685)
                     /locus_tag="KPG09_000324"
     CDS             complement(20663..21685)
                     /locus_tag="KPG09_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG0964953.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(21691..22704)
                     /locus_tag="KPG09_000325"
     CDS             complement(21691..22704)
                     /locus_tag="KPG09_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG0964954.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(22832..23770)
                     /locus_tag="KPG09_000326"
     CDS             complement(22832..23770)
                     /locus_tag="KPG09_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0964955.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWSQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            24040..24504
                     /locus_tag="KPG09_000327"
     CDS             24040..24504
                     /locus_tag="KPG09_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964956.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTGKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(24616..24933)
                     /locus_tag="KPG09_000328"
     CDS             complement(24616..24933)
                     /locus_tag="KPG09_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0964957.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(24946..25677)
                     /gene="chbG"
                     /locus_tag="KPG09_000329"
     CDS             complement(24946..25677)
                     /gene="chbG"
                     /locus_tag="KPG09_000329"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBG0964958.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVVEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(25703..27013)
                     /locus_tag="KPG09_000330"
     CDS             complement(25703..27013)
                     /locus_tag="KPG09_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG0964959.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNNGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLVAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(27090..28403)
                     /locus_tag="KPG09_000331"
     CDS             complement(27090..28403)
                     /locus_tag="KPG09_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0964960.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(28425..28736)
                     /locus_tag="KPG09_000332"
     CDS             complement(28425..28736)
                     /locus_tag="KPG09_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0964961.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(28936..29640)
                     /locus_tag="KPG09_000333"
     CDS             complement(28936..29640)
                     /locus_tag="KPG09_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0964962.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(29735..31096)
                     /locus_tag="KPG09_000334"
     CDS             complement(29735..31096)
                     /locus_tag="KPG09_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0964963.1"
                     /translation="MKDRINLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAIYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     MSE"
     gene            31595..32740
                     /locus_tag="KPG09_000335"
     CDS             31595..32740
                     /locus_tag="KPG09_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0964964.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQDVINKKYIVYRIGGDEYIIILKNLSHKEINSIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKKKINK"
     gene            complement(32830..34548)
                     /locus_tag="KPG09_000336"
     CDS             complement(32830..34548)
                     /locus_tag="KPG09_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG0964965.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(34553..35542)
                     /locus_tag="KPG09_000337"
     CDS             complement(34553..35542)
                     /locus_tag="KPG09_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0964966.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            complement(35638..36687)
                     /locus_tag="KPG09_000338"
     CDS             complement(35638..36687)
                     /locus_tag="KPG09_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0964967.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTYNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            complement(36964..37632)
                     /locus_tag="KPG09_000339"
     CDS             complement(36964..37632)
                     /locus_tag="KPG09_000339"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBG0964968.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            complement(37638..39011)
                     /locus_tag="KPG09_000340"
     CDS             complement(37638..39011)
                     /locus_tag="KPG09_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBG0964969.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            complement(39008..40786)
                     /locus_tag="KPG09_000341"
     CDS             complement(39008..40786)
                     /locus_tag="KPG09_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBG0964970.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            complement(40903..41226)
                     /locus_tag="KPG09_000342"
     CDS             complement(40903..41226)
                     /locus_tag="KPG09_000342"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBG0964971.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            complement(41213..42190)
                     /locus_tag="KPG09_000343"
     CDS             complement(41213..42190)
                     /locus_tag="KPG09_000343"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBG0964972.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            complement(42205..42768)
                     /locus_tag="KPG09_000344"
     CDS             complement(42205..42768)
                     /locus_tag="KPG09_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBG0964973.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            complement(42820..43311)
                     /locus_tag="KPG09_000345"
     CDS             complement(42820..43311)
                     /locus_tag="KPG09_000345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBG0964974.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            complement(43314..45239)
                     /locus_tag="KPG09_000346"
     CDS             complement(43314..45239)
                     /locus_tag="KPG09_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBG0964975.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            complement(45241..45552)
                     /locus_tag="KPG09_000347"
     CDS             complement(45241..45552)
                     /locus_tag="KPG09_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964976.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VRKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            complement(46182..46457)
                     /locus_tag="KPG09_000348"
     CDS             complement(46182..46457)
                     /locus_tag="KPG09_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBG0964977.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DITVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            complement(46572..47981)
                     /locus_tag="KPG09_000349"
     CDS             complement(46572..47981)
                     /locus_tag="KPG09_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBG0964978.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     SLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGGIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            complement(48351..49013)
                     /locus_tag="KPG09_000350"
     CDS             complement(48351..49013)
                     /locus_tag="KPG09_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964979.1"
                     /translation="MIDVSIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            complement(49267..51486)
                     /locus_tag="KPG09_000351"
     CDS             complement(49267..51486)
                     /locus_tag="KPG09_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0964980.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYALNQDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKITISVPLLNNDK
                     VVGVLYCTYNINTLMKLINVSFYENNSISYVVRNDGTIILHPQEDSLSKNIYKLLKQD
                     NDIREINRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSEQIINRAIYAVLFIILIFIAIIFYIIYIKKSNEKEILSLAYEDKVTHIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGKNKRIRKIDSIRNNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LSEWKKQGKKIVPVSINLSRVELYQPDIVKFINKTIQMYNLSSDLIEIEITETVAINE
                     LNILKNVLNELRKYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLNGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(51762..54416)
                     /gene="adhE"
                     /locus_tag="KPG09_000352"
                     /gene_synonym="adhC"
     CDS             complement(51762..54416)
                     /gene="adhE"
                     /locus_tag="KPG09_000352"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG0964981.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYTQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDESADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDNAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRSPGKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEEPKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
BASE COUNT        18426 a         9442 c         6734 g        20147 t
ORIGIN      
        1 tctcagaact tagttacaag ttctaaaacc ctatttgttt agtgtaaaaa acatcataaa
       61 atttagctta aatcttttca cataatatta ctctttttct aagtgataat aaaatatatc
      121 tattcctcta aaaactgtaa aaattcctga ttaaatagtt taagctcatc atagaatata
      181 gcatgaccac tatactcaaa agtatataaa attgaatctt ctattttttc attcataaaa
      241 actgcaaatt catacggaca tatttcatct aatttcccat gaaatatacc agtaggcact
      301 cttacacatt ttaaatcatt aaataattct tcatctctta aagatacagc agttccaata
      361 gtgccaatac ctgatgcact aaaaccaata tcattaaacc atcttctaaa gctttctggt
      421 ggatttgatg caaatacttt ttctccaaaa tcggttacca tttctggtct atcagtacat
      481 gcttgtgcta tcaatttatt tacatcttca cgtgtcatac catatggaaa ttctggaggt
      541 ctttgtacaa atgatggtgc agcagctgca gctaatacca attttgacac tccatatcca
      601 ttaaataaac tcatatacct aagtacgatt gctcctccca ttgaaaaacc aacaaaggta
      661 aagtctttta atccaatagc atgtaccacc ttataaatat cgtctgctaa ttggctataa
      721 gtatatcctc ctgatgttgc atcagattta ccaaaacctc ttaaatcaat tgaaactgtt
      781 ctatatccga gttttggcaa tatatttgtt tgatattcaa atatcttatg tcctaagggc
      841 caaccatgta taaaaagtac agttttttta gcactcggat tcaaatcata tactgctatg
      901 tttatatcat tcacatatac ataatacata tcaacagcaa ctcccttcaa gaaaatgtat
      961 gatggttatt ttatatatat gaataaaatg atgaataact aaatttttaa atttatttac
     1021 aatacttctc tattgccttt tcaaataaac ttacatgaag tttttcatct aggattattc
     1081 tatttaatat agcttttata taagggtctt caataagact tatatcttta taataattat
     1141 taatagccac atgctccaaa tgcaaatcta gttttaatct gttacaaata gaatttccat
     1201 aacatacaaa accaccattc caatatgcac cacaagaact tgtagaacct ctatatacag
     1261 gatttccacc caataatctt atagtctttg ctaatatctc catatgtaac atttctgtta
     1321 tagatatatt tatccacatt tcacttaact ctctatgcgt ttcatctaaa tcaaaattat
     1381 ggtatagata ttgatttaca ctcgtaaatt cacttgatac tccggcatag tcatccatca
     1441 ataattctgc ataatactta tttagaccta aaacttttat ctctggatat ggctcatcac
     1501 ttgaatatcc atttcgcttg tgcatgtcgt aatgtgcatc actcataata tctcctcctt
     1561 taaatttaat acttgatatt aattgtatgc atcacgactt tttcatataa cttagataat
     1621 aaaatctatt atattatttt ctattataat ttatgtgtca attcacccaa atttatttga
     1681 tatgcaatat ttctttcctt agtgctacat atgtaataaa aacatatata caatatatta
     1741 ctacaaaatt aataagcgta atggctacag catgtaagat aggaatctga atattaaaaa
     1801 atactggagc atcaaaagaa atattaaaca tgttgtatga aaaaactgat accaatattc
     1861 caccaaatat tactgcagga attaatggaa tcgaaaaata taaacaaaca tgtttcttta
     1921 agatttttat tacttcacta tcaatcatcc ccaaagttcg aagcattcca tagtgtttta
     1981 tgataatgtt attttcgctc aataattgaa tagagagaat gactgtacca ataatgcata
     2041 taatgagtgc caaataaaaa agtggtaata tcaagataat ggcttgacca gtagattgca
     2101 tagcatactt tccacttata taatccttat tatcatatcg taatttagaa acaaagcctt
     2161 gattacctgc tactactact ccagaattaa gattttctaa agtatcaaac tttttctgaa
     2221 actcagtaaa ataagcacta tccaaaggct cacttgtaat agcaacataa agactataca
     2281 gaggtttcat attatcaatt aaataatctg gaatgacaat aatatattct tgtccatttc
     2341 catatcctcc atactgtgaa aagttctcta tataaatacc tgcaatactt aagtccttat
     2401 ctgatattgt cagtttatat ttttctccaa tataattctt atatgcatca gcaagataag
     2461 gcatacaatg gatgataaat tgatttttgt ccatctttat tgggtttaaa cccaacatat
     2521 ttcttagtat attgtaatca ctatatttca taaatgtgtc aaactggttt tctgcaaaag
     2581 tataatcctt atattgtaaa tctcgcccta tatctttcaa gtatttagaa aggatattat
     2641 tacgaacctt cataaaggat gttttttctt ctgtgtaaag agtataagaa taatcctttt
     2701 tcacaccttt cgtttctgat aaatatcctt catatgaaga aaaatcatat gattcattcg
     2761 ggtgaaaaat actcacatca aattgttgta aatccactga tttattcatc acagaataaa
     2821 atgagttagc tattccaata caagcaattg atactgtaaa aagagttgcg ataataccca
     2881 ttataatgct tattccattt atctttgaag ccagtagacg atacaaaaat aagtgagatt
     2941 tcttatattt ccaattcatc cctttttcaa acatattctg tgcaatagat ccaaatccca
     3001 taaataatac gattagacaa aatacaatca acaataaacc aagaaataca tctgttaatc
     3061 taccactagg tattgaaaag aaaaaaatac ccatacctcc acacaaaaca gacacacaaa
     3121 acataataga cttcgttttt gtttggtgaa ttaatacatt ttcattatat cgatcataat
     3181 taagcaaatt aattaatttc attttaacaa gttttttatt actttttaat tgagcaatta
     3241 aatatattat gcagaagtat aaaaaagtca tcccagcagc ttttaatgaa aaatccggag
     3301 ataaagaata ctttccatga aacatattaa taacaataga ttccaaaacc ttagaaacaa
     3361 atatccctag aatccatcca acaataagag cagaagcacc taataaaaga ttctctataa
     3421 aaagcatttt aacaatttgt tttttatcta ctcctaatat catataagta cttatttctc
     3481 tgcttctttt tttcaatata aaattcatca tataactaac aaaccagcct aaaataaaag
     3541 taataatgaa cgaaaatatg ataatcatat aagaaaactc atcgcttcct ccattattaa
     3601 gtatactaga aagttcttgc attacgtcgg aaaatatcaa agcgttaaaa gcataaatca
     3661 aagctacaga gcctattaag gaacaccaat aaataaggta ctttcctgct tgtcgtcttg
     3721 cattacgaaa agataatcct ttcaacataa tcaatctcct cccatcaaag aaagtgcatc
     3781 caaaatttcg tgatagaaag taagtctgga tttttctcct tttctaagtt cgttaaaaat
     3841 tttcccatcc cttagaaata aaattctctc agtataacta gctgaaatag cgtcgtgtgt
     3901 gacaagaaga atcgtagcct ctaatatggt gtttatagtt tccaaaagtt ccattaaagt
     3961 tttagatgaa ttagaatcca atgcaccagt tggttcatct gccaaaataa gtttgggctc
     4021 tgttacaact gcacgagcta ctgcacaacg ttgcctctga ccaccagata cttcataagg
     4081 aaacttcgaa agtatctctt caattttcaa ttgtgtggca atattataca cactattagt
     4141 aatttcaaca ggagaaactt tttttattgt aagtggaagt gctatatttt cttctaatgt
     4201 cagagtatct aataaattag aatcctgaaa aacaaaacct aaattatcac gacgaaaaac
     4261 agttaagttt tcttcggata gttcagtaat atcttgattt tcaagataaa tttttccagc
     4321 acttactgta tcaattgtag caatacaatt taacaaagta gttttaccag agccagaagt
     4381 tcccataata ctgacaaact ctcctgtatt aatttgaaat gacacatcat tcaaggcctt
     4441 tgtaagtatt ttctgattgc cataatattt ctcaatgttc tctactcgta atagcatgtt
     4501 aaagtcctcc ttagtcaaca atttttccat ttttaattat tattgtctta tctgaaaaat
     4561 tcaagatata tacatatatg ctttctttag agtcttgaat ttgaatagta ggttcattag
     4621 atgaaattag agattttttt acttcaatat cgtattttat tgttaattca ttatcatctc
     4681 catttttatt agtatcttct acatttttat ttaaatcaat gttattatga ttaaagggac
     4741 cagtaaactt tacttctgta atagttccat tttttctaat aaaagaagca tattcagacc
     4801 ttagtggtat gatacttcgt gagaaaaaac ccatcgaatc caatcctgta attgaaataa
     4861 acaaaacgag tacaacacat aatatagtta atatcctttt tttcatatgt ttctccctcc
     4921 tttctattga tattgtagcg taaatagtaa tctgaatata tagaagttac ttaactttat
     4981 cttacagttt tgaaagataa agaaatagca gcgcattttt agttgcactg cttctgcata
     5041 ttgatagctg tatttattgg gaaattcaat gttattgctg tgtattcacc aaggctggag
     5101 gacacatcta tatcaattcc cattttctta gacagttcat aacaaaggta aagaccaatc
     5161 cctgtagaat tgtttgtagt ccttccatta ctgccagtaa aacctaattc aaaaattcga
     5221 taaatttcac tttctttgat gccaatacca ttatctttta tagtaaactt aacactcttg
     5281 tcttctttta ttgcaaaaaa tgaaatttca gctgaatcac ttttgtactg aacactattg
     5341 ataataattt gatttaaaat aaaaactatc cactttttat cagtatatac actatactcc
     5401 aaatcctgta tattaatatg aatattattt tgtataagaa gcaatttatt ctttgctaat
     5461 acttcttcta caattgactt taatgttatt tcagaaatta aataatcttt ttcaacatgg
     5521 ccaaggcgag catagtacaa tgcacgctca atatgacgtt ctatttcttc catttgaaaa
     5581 cgaatttttc gtgtatgttc atttttataa ttttcacata taagttctga tgttgtaaca
     5641 ggatgtttta tctcatgtac ccattgttca atgaattgtc tatattcgta catttcacga
     5701 tgtatttgag aaacatgatc tgtcatagat ttcaatgata atctaataat attgtagtat
     5761 tcacgttcta tgttagaagt aggtttttca atcatttcaa taaacagata ttttttatcc
     5821 aattcgtcta caatgcgacg taacttaata atttcttttt tctgtttttg ataacaaaaa
     5881 catatccaac aaaataaaat tgcgacccaa gcggctataa gaagagtcaa ctctccttga
     5941 ccaatcccta taagatgaag atatagcact aatgaaattg cacaacaaat atttaagagt
     6001 ataactaata ctttatcttg aagaaaatta caaaacctca tatcctatat cccatccccc
     6061 gttttgtaat gattaagtca gtaataccta taccccttaa cttttctcta agccttgtaa
     6121 tgtttacact taaagcatta tcatcaatat gaatatgatt gtcccataaa tattctacca
     6181 aatctatacg aggcacaata ctccctacat tctgaaacaa ataatacatg atttttagtt
     6241 cagttcttga aagttctata acagcatttt ggtattctac tgtagctgaa ataaggttga
     6301 gtataatctc tctatattta attttctgtt tatctgatga atctactctt ttaagtaggt
     6361 tctttaccct tgctaaaagc aaaggtaagt tatatggttt tgtaatatag tcatctcccc
     6421 ccatagtcaa accatttagt tcatcaattg cactatctct tgctgtaata aaaataatag
     6481 gtatagtaga acaattccta atctgtctac ataaagaaaa accatctata ttaggcaaat
     6541 tgatatccaa gagaattaag tcaggtgttg attcttggat tacttgaagt acatcacaat
     6601 tatttgtatc caatgtttgt gtactatagc cattattttc aaagaattgc attaattcct
     6661 tacagatgaa cacatcatct tcaattatca aaatattggt taaaataata aatacctcat
     6721 ttctttagtt tatattgctt taattttgta agtaatatat ttttccatat attacttaca
     6781 aaattacttt ttaattattc atcttttcca gctactgata gctctttaat aacaactgaa
     6841 ggtgaaccta tactactcat tggaaattct aaatcttccc ctataacttc aatatctttc
     6901 aacaggtcaa aatagtttcc tgctactgta atttgctcca ctggaaatac tttttttcct
     6961 tctttgatat aaaagccttt ggcagctaaa gaaaagtctc ctgttactga atttgcacct
     7021 gaatgaagcc ctgcaaagct agtaaccatc aatccttcat ctatatcctt tataatttca
     7081 tctaaagatt tatctccttt atctatataa aaattagttg gatatatgct tataggtgat
     7141 gaatatgaag attttattcc atttcctgta gtctttacac ctgccttatt agccgttttt
     7201 aagttatgaa gtaaagttat aagctttcca tttaaaacaa cttctttttt aaatgtagct
     7261 actccttcat catcaaaagg agtagatgcc ataccatttt ctaaaagtgg gtcatcaact
     7321 attgtaacta taggagatgc tatcatgtct ccttctctat ttttcaataa agataatcct
     7381 ttttgagctg aatctgcatt aaatatccca gaaaatgtgc taagcaaaga taccatagct
     7441 tcattaagca atatagtttt atatttacct gatggaatac tcttaccacc tactttagat
     7501 aaagcattat caacaccaag ttttgatatc tcacaaggtt ttatttcttc aatagaattt
     7561 gctctattat atcctattcc atcatatttt tgtccattgt cttctatgat aggaactaca
     7621 aaccctataa gcatattagt tttattgctt aaattaagac ctttagtatt agatatacca
     7681 ttacttgata cagaataaga tatagtacat ttagataaat ttaccacctt atctgaataa
     7741 gctttagtct ctctttccat ttctaaagct aaatcaatca acttatctgc ctctaaattt
     7801 tctagctctt tagaataagt ctttacatca ttatagtgct tatctccttc atatataaat
     7861 tgtacatctt cattttcaat agtgtttact ccatctttta cattttttat caacatatca
     7921 acagctttat catctaaaat ttctgtataa gaataaccta tctttccatt taccttacct
     7981 ctaaaagata aaccaaaaga tttatcaaga ttgtatttct caacttctcc ttcatatata
     8041 tttatactta aattttctcc tgtataataa tatatttcac actcttcaaa accttcactt
     8101 agagcctttt taaataatat atctttaaaa tttttaagtt ccacaattat ccctcctatc
     8161 ttccacctac agtaatttct tttaccctta tcataggttg gcctacatta gttggtatac
     8221 ttcctgatga tgagccacac atcccctgag cttgttttac gttattccca accatatcta
     8281 tattcataag tatatcgcta ccttttccaa ttaaactagc acctctcact ggctcttgta
     8341 tttctccatt ttttattaag taaccttctg aaactgcaaa attaaactct cctgttacag
     8401 gatttacaga accaccaccc atttttttag cataaagtcc atctggtatt gactttataa
     8461 tgtcttctgg tctatcttct ccagcagcaa tataagtgtt agtcatcctt gaagttggtg
     8521 caaatttgta gttctgtcta cgagaacttc ctgtaggatt cattcccatt cgtctaccat
     8581 taagtttatc tatcatataa gacttcaaaa ttccattttc aatcagtata ttcttttgag
     8641 ttggatttcc ttcatcatct atattcatag aaccccagta gtttggtatt gtaccatcat
     8701 ctatagctgt aatctttgtg gaagcaatct gttgacctaa tttatctgca aatacagagt
     8761 tcccctttgc aactgatgta gcttccaaag aatgtccaca agcttcatga aatattactc
     8821 caccaaatcc attatctatt gctaccatca tattacctgc aggacaattt ttagcatgca
     8881 acatagtatg cgctactcta gctgattctt ttgcataata ttctgggtct atttcttcaa
     8941 atatttcaaa ccctttacaa cctccaggac cttcaaatcc agtttgattt tcattaccct
     9001 tagatgcaat tgaacttatc cctagtctgg ttcttattct tctatcctca acatatatac
     9061 cttcagtgtt tgcaattaat attttttggt ctttatccaa atagctaaca cttacttgag
     9121 atatatcatt actaaattct tttgcactct tatatgcaac cttcataaca cttactttat
     9181 ctctatttcc tattgaattt ggataaattt ttatattatg tatgttattt attttaatac
     9241 tgtcatttaa tattacactt tttccatctt ccaatttacc taaagcaaca gcagctttgt
     9301 atgctacatc caacagacta tttaatgaat tgtcacttgt ataagcatat atactcttca
     9361 atcctttaaa tattcttatt cctataccat aatttcttcc acctattgca ttttctactt
     9421 tattatcaat taaactaata gaattattta tagtgtcttc ttcaaaaatc tcagcaaaat
     9481 ctcctccagt aattagacac ttttctaata ccttagaagc tatatttttt gaaagcataa
     9541 atttacctcc tactttaaat ttttaattaa cttttattca atctttttac ttgttaacaa
     9601 acattatata ctatattact taaaataagg taattatgaa tatattatac atatgtttta
     9661 tactaaaatg ttatcacatt ttatctatac atacccatat aactagataa ttaaaatgta
     9721 taatattatt tgctatagaa aataatacac tcatacataa taaattttag gagtgaagta
     9781 tatgaatcaa gaatatgtaa aaggaaatcc tatttttact aatattaata aagttccaag
     9841 acaatttcca taccttactg atgatattga tactgatgtt ataattgttg gtggtggcgt
     9901 aactggatgt atttgtgctt actatctagc taaaaacaat ataaagtctg tcattcttga
     9961 aaaaggaagg attgcacatg gaagcactag cgtgacaact tctctattac agtatgaatt
    10021 agatgacaat ttgatagatt taacagaggt tatgactcta aaagatgctc taaaagcata
    10081 taacctttgt acttcagcac ttgaagaatt agatacattt ataaagctat atggaaataa
    10141 gtgtgattat gccaaaagag atactctact ttatactgcc aacaaacttg aaattaaggc
    10201 tatcaaagaa gaatataact taagaaaaga aaatggattt gatgttgaat atattgatga
    10261 atccacaaac ccttttagct ttgatttaaa atcaggattg atagcaaaga atggaggacg
    10321 agaattagac ccatataaat atagtcacca tttgattgat gtaagtttaa aaaatggtct
    10381 tcaagtatat gaaaatacag aagtaaaaaa agtagattta tcaaatgata aggttactgc
    10441 tgaagtatcg tatggataca aagttcatgg taaaaaatta attgtagcta cagggtacaa
    10501 tacaagttta tttacaaaaa gaaattttgc aactaagtca aatactttta atatagcaac
    10561 taaaccactt aaaaacatag cttcttggaa aaacaacatt ttaattagag ataactgtga
    10621 cccatataat tatctaagaa ctactaaaga caatcgatta attataggtg gtgaagatgt
    10681 aagttttgat gatatagaaa atgaaacttt agccaatgaa aagtatgata ttttagaaca
    10741 aagattgaaa agtatgttta aggatataaa agacattgaa gttgaatata aatattgtgg
    10801 ttgttttgct tcaactttag ataatttagg ttttattggt ccagataata aaaataacaa
    10861 tctatggtat tgcttaggtt atggagcaaa tggtatactc tttgcaatat taggaggtat
    10921 aatgttaagt gaattatatc ttggcaaaca aaataaaaat atgaaattat ttaaagtaga
    10981 tagatttgat aaatagatat tgtatattaa aagactataa aaagatgaaa ctttataatt
    11041 ctaaaagtta actttgatac agtcttttat aatgttcctt ttaaaaatac tttatttata
    11101 attaaatata aagtaaaatg ttaaaatata actgataaaa tttagtcata agataatatt
    11161 taagtttatt tgatgaaata tggaggaatt attatgaaag aaatactcgt tattgctccc
    11221 actaaaggaa cctatgaaaa atcaattcac atagttaaaa aaaacaaata tacaaatatt
    11281 gatgttgtat ttggcaatct aaaagaaggt atacctcttg ctgaaaaaag tattaatcat
    11341 ggtactagaa ttatcataag cagaggtgga acttacaata tgttaaaagc tacttacaat
    11401 atacctatag ttgaaattaa agtagatgct tatgatatta taaaaagtta caaagaggta
    11461 aaaaattcca acgaaccatt tggaataatt ggatttaata atgtcatcta cggtttcgat
    11521 attatagaag aaatattgaa taagaaaata actatgattg aaattgaaaa agaagaagaa
    11581 atttatgatg ctatagaaaa gtacagaaag aaaggtataa acacatatat aggagatacc
    11641 actgttgcac atattgttaa gagacttaat tgtaagggaa tattaatcga atctagagaa
    11701 gaaaacatac ttagagccat tcaacaagca gaacaaatac tcgaagcaac caaggatgaa
    11761 caaaaaagaa gattacagat agaagctatg acagattttg tacatgatgg aataataaca
    11821 gttgataaaa attttaaaat aacattattt aataagagtg ctgaaaaaat atttggtata
    11881 aaaaaagaaa atgcacttta tcataatgtt gtagatgtta tcccaaacac agttcttcca
    11941 caagtagttg agactatgga acctcaaatt ggtgaaatac aaaatatagg gagtacaaaa
    12001 attattacaa atagagttcc aataattgtt gatggccaga ttcaaggggc tgtttcaaca
    12061 tttcaagata gcaaggagat aagttctttt gaacatacaa taagaaggag cttattaagc
    12121 aaaggtctat ctgctaaata cacttttgat aatataattc atgctagtga aaagacaaaa
    12181 gattgtatag aacttgcaaa aaaatatgct tgttatgata ctcctatgat tattactgga
    12241 gaatctggag ttggtaaaga attattttgt caaagtatac ataactatag taagcgtaaa
    12301 aatgcacctt ttgttgctgt aaattgtgct gccatacctc catctctaat agagagtgag
    12361 ttttttggtt atgaggaagg ttcatttaca ggtgctagaa aaaaaggtaa agcaggtgta
    12421 ttcgaactag cacatgaagg tacaatcttc ctagacgaaa taagtgaaat ccctcttgaa
    12481 cttcaaggtc gattattgag ggtattgcag gaaaaacaag ttatgagaat tggtggtgat
    12541 aaagttattc ctatagatgt aaaaattatc tgtgcttcaa ataaagactt aaaaaatatg
    12601 atgcgcaatg gtttatttag acaagacctt tattttagaa taaatatact aacattatat
    12661 cttccttcat taaaagaaag aaaagaagat attataaaat tatcagaatt ttttatacat
    12721 aaatattcaa gcaaatacaa taaaacgcct ttaattatca ctccaaatat taaaaaggca
    12781 cttcttgaac gagaatatga aggtaatata agggaattgg aaggtatgat ggaaagggct
    12841 gttattgtgg aatcatttga ctcgatttta ttagaaaagg ttagcaatga atatacaaat
    12901 aatatagttg ataaagataa tagtgatttt gaatgcagta atttagattt aaaaacttta
    12961 gaaaaaaaat atatctataa aatttataac gaaaataata gaaatactac aaaaacctgt
    13021 gaaattctaa agataactcg ctccactcta tggcgtaagt taaaagaaat agaagatgat
    13081 gtttaaaaat aatacatacc tatatatatc aaaacgtttt atgtttaatt ttaatacata
    13141 aggcgtttat ttgttttcaa taaaaaatac aattatatga taaaaagaat ctttgtaagt
    13201 gaaattctca tcaatcatac ctttaattat aagtaatcta cagcatttca atcgattatt
    13261 aaaaataatc atgataacta aaaatacaaa ctctatatat tggcatagta attgctttta
    13321 taatacctat ataaaaatga aagggagtga tactagatgc acatcttaaa atcagtgaaa
    13381 aagataccag gaggacttat ggtctgtccc ttgttattgg gagcactatt taatacagta
    13441 tgtccacaag ctcttgaaat tggaggattc acaacatcct tatttaaaac tggtgcaatg
    13501 agtatacttg ctctattttg tctatgcaat ggagctcaaa tcaatatcag gcaagctggt
    13561 aagccattga ctaaaggtat agtacttaca gcaacaaaat ttataattgg tgcagtatta
    13621 ggtattattg ttagtaaatt tattggacct aaaggtataa ttggtattac acctcttgcc
    13681 atagttgcta ccatgactaa ttcaaatgga ggtctatttg ctgctcttgc aggtgaatat
    13741 ggtgactcca cagatgttgg agcaatatct atcttatctc taaatgatgg tccttttttt
    13801 acaatgcttg cttttggagt aactggactt gcaaatgtac ctatagtagc actatttgca
    13861 gcacttatac caatactact tggatttata cttggtaacc ttgatgaaga tatacgagaa
    13921 tttcttgcac ctggtacaac attattaatt cctttcttcg cttttccttt aggagcagca
    13981 ttaaacttta atcaaattat aactgctgga ctacctggag ttgtacttgg tttaggagtt
    14041 actataataa caggtatggg tggctacttt gtaatgaaac ttatacgtgc agaacatcca
    14101 gcagtaggtg cagctacagg tacaacagcc ggaaatgcag ctggtactcc tgaagcttta
    14161 gcagctatag accctactct agctacaata gcagctgttt caacagtaca ggttgctgct
    14221 gctattattg ttacagcaat atgttgtcca atgttagttt cctatctaga taaaagagaa
    14281 aaaaagaaaa aatctatttt taaaacaaat aaaaatattg aagtaaatta atacttaagg
    14341 aggtaaaatt aatgaatgca attattcatg caaatgaaaa agataatcta gttacatgtg
    14401 tcagaccttt agttaaaggt gaaaaagttt caattgatgg aaagtcttac atagtaaatg
    14461 ataatatacc tgtctttcat aagatggcta cagaagatgt aaaaaaaggt gatgttgtat
    14521 acaaatacgg tgaaattata ggaattgcaa cagttgacat aaaaactggt gactatgttc
    14581 atgtacataa cattgaaagt acacgtggtc gtggagataa aaaataggag gaagttaaaa
    14641 tgaaattaat gggttattta agagaaaatg gtcagtttgg tattcgtaat catgttttaa
    14701 ttattccaac atctgtatgt tcaagtgaga cagctacaag aatagcatca cttgtgcctg
    14761 gagcaatagc aatccctcat caacatggat gttgtcaaat tggttctgat atagagctta
    14821 cagcaaagac attaatagga tttggaaaaa atcctaatgt tgccgcagtt ttagtagttg
    14881 gtcttggatg tgatggtatt caagcaaaag aattagcatc tgaaatagct acaacaggaa
    14941 aaaaggtaga ttacgttgtt atacaagaat gtggtggaac attaaaaaca gtttctaagg
    15001 gaactgaaat agtaagtaaa atggctcgtg aagtatcaaa agaagtaaga gttgaatttg
    15061 gtatgagtga aattacatta gcacttgaat gtggtggttc tgacccaaca agtggaatag
    15121 cttcaaaccc ttcaataggc gttgcatcaa accttcttgt agatgaaggt ggaagtagca
    15181 tactaagtga gactacagaa gtaattggtg cagaacattt gttggctacc cgttttgaag
    15241 atgaagaaat gaaagataag tttcttaaat ttgtaagtga tgtagaaaaa agagcaattg
    15301 ctatgggaga agatttacgt agtggtcaac ctactccagg taataaagct ggtggacttt
    15361 caacaattga agaaaaatca cttgggtgta tgtataaggc aggcaataag ccattcaaag
    15421 gtgctcttga atatgctgat attgtacctc ctgataaaaa aggattatat tttatggata
    15481 ctccaggtca agatatagat tctatcactg gtatggtagc aggtggtgca caaattgtaa
    15541 tcttttcaac tggtagaggt actccaacag gaagccctat ttcaccagtt attaaaatca
    15601 ctgggaatag tgatacatac aacaaaatgc cagacaatat cgatataaat gcaggtagaa
    15661 ttataacaga tggtgctaaa atagcagata taggtcaaga aatatttaat gaaataatag
    15721 aagtctgtaa tggtaaacat accaaagctg aaagtcttgg tcatagagaa tttggcattt
    15781 acagaatttc atctacattt tagattgtaa caaaatatct tttataaata taatacttat
    15841 gtagatacat aagctaaaag ttctttctaa tcctataaaa attttctata ctgaaaagga
    15901 actgtatcaa aattattttg aaaacagttc cttttttaac ctcttttact caaaatatta
    15961 taaaataaga tttaaacaat aaacgtgtct tgattaatct ctaagaagac ataaataaag
    16021 ctaaatgtaa agtgagataa ataaaattta atatctttgt atgaaaagat atttggtata
    16081 atatgtctga gagaattctt atttgtaaac aaataaagga tggaggatga aacaaaagaa
    16141 tgaactttga aaacacaaaa aataaaaaaa taatagctac tttactattg acactccttt
    16201 taatcgtgtc attaacatgt gtcaatttta ttatgaatct aaataaagta acacataatg
    16261 aaacagaacg atatcttgta gaagtttcac aaagtatttc cactacaatt aatgtgaaat
    16321 gtaaaagtat atttaggaca ctatattcta tgggagatac ttattttgag attaatgata
    16381 gtgaaaaaga agtcaataga tacttatcaa aaaaattaaa attatttgaa tttgattggt
    16441 taggtattat agattctaat ggaaattttg taagtgccct taaagataca ggtaatgtta
    16501 aaggacaaga cttttataga aaagcccttc aaggtaaaga aacaattact cctgttaaat
    16561 cacaatctgg tacatatgga attttatatt ctgtaccact atacaatggc gataaaattg
    16621 ttggagctgt tgctgcttgg aatactattg atactatgag aaatttatta agtgtagaaa
    16681 gctttcatgg tgaaggattt tcccaaatta tcgaccaaga tggaaatttt attgtaagtt
    16741 cagcaaacaa aaatgctcca aaaaatgtgg taaatttttt tgacatgatt aaacaccaag
    16801 gaaaacaggt tgataaaaat aaacttgaag aaatgaaaat aaacttaaaa aagaacaaaa
    16861 ctggttctat gtattataca ttaaatgatg gtatttctaa ggcaatgcat tatcttccat
    16921 tacaagaaga aaacttatac ctcctttcag ttgttccaac aaaaacagca agtgcacagt
    16981 ttgatttact tctaaaaaaa gcaatcttca tcaatatttt aattgttata ctatttttat
    17041 tgcttattat attgattatg tttataaatc aaaagaacag ggcacaactt ataaaaatcg
    17101 cctatgtaga ctctgtaact aaaggttaca gtaaagcacg ttttgatatt gaagctaaaa
    17161 aaattataca gtcatcttca tcagaaagct attcattggt atctataaat attcagaaat
    17221 tcaaacttat taatgattct tttggtagtg aagcaggaga taaaacatta aagtacatac
    17281 atgatgtcat tagtaattat ctagaaccag gagaattgct ttgtcgagtt tctaatgacc
    17341 agtttaatgt acttattaaa actattcctc aaaaagaaat tcttaagtac atagaaaata
    17401 tcaccaatga tattaatagt ttcaataaaa atgaagaatg taaatatttt atagctctat
    17461 ctataggtgt atataaaatt gatgacccaa atctaccttt agtttcaatt caagaccgtg
    17521 caaatgttgc acgaaaaaat aataaaggaa acattggaaa tcatccactt tatacatgtg
    17581 ttttttatag tgatttagag cgtttaaaaa tgattaaaga aaaagaaatg gaaaatagaa
    17641 tggaggaagc attaaataat aacgaattta tagtatatct tcagcctaaa ataagcctca
    17701 aagataatac tattgtaggt gcagaagcat tagtaagatg gcaagaccca aataaaggcc
    17761 ttataccacc agacgagttt attcctttct ttgaaaaaaa tggttttatt acaaaactcg
    17821 accactatgt ttttgaaaaa gtatgtagta tgataagaaa atggatagat aatggagaaa
    17881 ctccaatgcc tgtttcagtt aatttttcac gtgctaattt aaataatata gattttttag
    17941 aaaaatacag aatgcttcgt aataaataca acattcctgc taagctctta gaaattgaat
    18001 taactgaaac acttgtattt gaaaatctcc aaaaattaat taatataata gaccaaatcc
    18061 atgaagaagg ttttcaatgt tcactagatg actttggaag tggatactct tcactcaata
    18121 tgttaaaaga aataaaagtt gacacattaa aacttgaccg tgcttttttt agctcaccta
    18181 atgcagataa tattgctgaa aatcatgtaa tagaatctat agttaatttg gcaaaaaaat
    18241 taaatatgaa tagtatatca gaaggtgttg aaacaatctt gcaaatggat tatcttaaaa
    18301 aaattaattg tgatatgata caaggatatg ttttttcaaa acctctgcct caagaaatat
    18361 ttgagaaaat gacttttgga aaaaaactag aagaaaccag tatggatagt tccgtttaaa
    18421 taaaataaaa aacttgtctc tataaaataa ttttaatcat tttataaaga caagtctttt
    18481 tattttccaa tttaatttta ttttctaaat ttcttatact cggcttgttt taaattccct
    18541 ttttaagaat aataatacga ttatcatagc aataaaatct gcaactggct gtgccaacca
    18601 aacaccatct aaattaaatt gaattggaag tacaatgatt aatggtataa ataaaataaa
    18661 ttgtcttaaa agagttaaaa acattgaatg tttaacttta cttatacact gaaaatacac
    18721 aggtcctaca atagaaatcc ccattatagg caatgtaatt agatttatgc ttatacctct
    18781 tactgctata tccatcaaat ctgaatcttt attaaatata cctacaaata cttctgggaa
    18841 aacttgtact attaaaaatc caaatgacaa tatgacaact gacactatga tagataatat
    18901 taaagttttc tttgcacgtt catattgttt tgcaccataa ttgtatgata ttatagtttg
    18961 cataccttga ttcaatccaa acacaggcat cataaacatc aataaaatag atgtaagtgc
    19021 tgtcatagcc ccaatagcta aatctccacc ataaaccttt agagaattgt ttgtaaacat
    19081 gtgagttata cttatagcta cttccatagc aaaaggtgtc attcctattg caaatacctt
    19141 ctttactata tctttattca attttaagtt acattttttt agttttaaag tggacttccc
    19201 tcttataaaa taatatgtta cccaaaagaa tactactatt tgacatagta ctgttgctat
    19261 agctgctcct tttataccta aattaaacac aaatataaat atagggtcca atatcaggtt
    19321 taatacacat cctacaatca ttgtccttgc tgctaatttt ggattacctt ctgctctaat
    19381 tgcattatta agagaaaatg caactaaatt aaatatactt ccaatcaata tgacactcat
    19441 atagtctttt gcataaaata ttgaatcttt acttgcaccc aaaataaaaa gtatatcttc
    19501 taagaaaact aatcctaata tagttataat taaagagata attatagata atgtcaaagc
    19561 attacctaat atcttctctg cttcttctct atttctttcc cctaatttta tagacaatct
    19621 tgtgctagca cctactgata ccaacattcc aaaagctatt attagagtaa atactggcat
    19681 agttacacca agacctgcaa tagctataga gccaatacct tccatagaac caataaaagc
    19741 tctatctact acattataaa gtgaagttac catcattgct attattgccg gtatcgaata
    19801 cctaaataat aaaatccata ttttttcatg tcttaatgct tcttgattct ccatgttgtc
    19861 accatttaca ccatgtcata agttatacaa attggtttat ttgttcttgg gtctgttgct
    19921 atttctgcat caatattaaa taattctttt agattatcct tcgtcataac atcatatgca
    19981 tcaccttcac aaactatttt acctttctta atacctatca tgtggtcagc aaatcttgct
    20041 gcattattaa gttcatgtat taccatgaca atagttcttc cttgtttttt atttagttca
    20101 tctaataatt ttagtatctc taattgatgt gccaaatcta aatatgttgt aggctcatcc
    20161 agtaataata tatcagtttc ttgtgccaaa gccatagcta tccatgccct ttgtctttgt
    20221 cctcctgata aatcttccat atttctatcc ttaaattctg caattccagt aacttctaaa
    20281 ctccaattta caatctctct gtcttcttta ctagaattac caaatccact tttatgagga
    20341 aatcttccat aagaaattaa ttctgaaacg gtaagaccac taggagcttg agggctttgt
    20401 ggaagtacag ccatatcctt tgctatttct ttagacttct ttttgtagat gtcctctcca
    20461 tttatatata ttttcccact attagggcta ataattctac ctatagtttt aagtattgtc
    20521 gattttccac aaccattagc ccctatgatg gtagttattt ttccttttgg tatatttaaa
    20581 tttaaatttt ttataatatc tgtgtttcca tatgatacat ttagattctt agtatttata
    20641 cagttcataa caacaccctt tcctaatcat ttaccaacat taaataaata aagtacggca
    20701 ctcctattat agaaactact atacctactg gtatttctat aggtgctaga agatttcttg
    20761 atatagtatc tgctattaaa agtaataatg ttcctatcaa tgctgatact ggtattaatc
    20821 ttttatgctt tggtcctatt atttttctac ctatatgtgg agctattaaa cctaaaaatg
    20881 aaatactacc tgctactgct gtcgatacac cagataaaat tactgcatat attataagtc
    20941 ttcttctttg tttttctaca tctacaccaa gacttataga tacttcatca cccaaattta
    21001 aaacatctat atatttagat ttataaatag ttatacccat aaaaatcact ataaatggaa
    21061 gcacagctat tacatatttc caactggttc cccaaatgct tcctgatgtc catgccataa
    21121 ccctattaaa ttcttgtgtt gtaaatttca attggaatat cgtcaaaatt gaagtaaaag
    21181 ctatgtttac acctatacca ataagtagta atcttgacga atttatacca cctttccatg
    21241 caagagaata tataagaaat gctccaaata aagctcctat aagtgcaaca ataggcattg
    21301 taaatacagt caagttactc ataccttcat atacattacc attcataagg tatatataaa
    21361 ctactacaaa caatgcagca cctgaattta ttcccaaaat tccagaatca gctaaatcat
    21421 tctttgtaac tccttgaagt attacacctg ctgttgataa tgcagaacca accaaaagtg
    21481 ctattactat tcttggtaat cttaatttaa atattgcaat ttcatgatta cgctgaccct
    21541 gccctaaaaa agtctttata acatccattg gttctataga aaatgagccc atatttaaac
    21601 tcaataaaaa agtgataaaa ataagcacca ttaaaattga tataacaagt aatgttttag
    21661 aatttatttt tataatattt ttcatagtac ctatttctcc ccctttctaa caaggtatat
    21721 aaatattggc actcctataa gtgctgttat agaacctact ggtgtctcat atggaggatt
    21781 tatcatccta cttaatatat ctgtatatac aagcaataca gctcctaata ctaaagagct
    21841 cggtattata tacttataat ctgaacctac tattccttta gctatttgag gaactataag
    21901 acctataaaa ataatattac cagacactga cacagatgct ccagtcatta atattacaag
    21961 tactatacat ataaatctaa caaagttagt tttttgtcct aatcctattg ccacttcttc
    22021 tccaagactt aatatagtta ttcttggagc cataataata gctaaaataa atccaatacc
    22081 accagctaca aatagcaatt ttataccttc ccacttcact cctacaagcc ctcctgatat
    22141 ccagaaactt aattgttgag ataggttaaa atacatagat attgctgatg ccaaagaaat
    22201 tagcaatgta ccaagagcga ctcctgcaag agctaatttt acattattaa ctcttcctct
    22261 tgatttagaa cttataaaat atataaataa tccacttata cttgctccaa taaatgcaaa
    22321 catcattaca ctaaaacttc catgtataag ttgaggaagt tttttttgaa gtaccagtga
    22381 tatcgaaatt gcgaatgtag ctccttgtgt aattcctata acagatggtt cagctatagg
    22441 atttctcgtt attccttgca ttattgcccc agaaactgct aaaaaccctc caacaagtgc
    22501 agctgctatt gctcttggaa gtctaacatc ctttactatt ctcatattta ttccatcttc
    22561 catttttatc aaactatcta tgacagttga tagattcata tcttttgccc caatagttat
    22621 ggataataca attccaaata caagtacaac tatacttact aatatcaaca aataagttat
    22681 atttttttta ctcagcttag tcaattatcc tacctccttt tcccttttaa tcgtcagtat
    22741 aatatcatct attaagtatt ttaattttaa gatttattaa aaataggtgt gaaaaatgaa
    22801 tatcattatc tcacacctac taagttaaga attactttgt taattcattt ttaactgatt
    22861 ctaataataa ctcttttcca attggattgt atgattgact gaaaaatgga gcagcatcaa
    22921 gtattgttac attaccatct tttactgctc ttatttgaga ccagatagca ctattttcta
    22981 aatctttttt atctgcttct gtagcaatta caactatatg gtctgcatct atatctgtta
    23041 aaccttccat acttaccatt ggtagtgtta ttccatcctg ttttggcata ttcttaggtc
    23101 tagctaattt catatcatca ttgattagag tacctattcc accatcacta aataccataa
    23161 attgacctga acttgccaac actggtaaat aagttttttc tccattcttt tctataactt
    23221 ccttacctaa ctttgtagct ttttcatcat acttctgtag ccaactttta gcttcttctt
    23281 ctttgtcaaa taactttgaa acatcagtta acttgcttct ccagtcattt gcataatctt
    23341 tcatcataac tacaggagct atttctttta attggtcata tattttttct tgtctttgac
    23401 tcattattat taaatcagga tttacttcta atatagcttc catatccata gtatccatca
    23461 ttgagtgccc aactatcttt acacctttaa gctcttctct tatgtaactt ggtaatttat
    23521 cagtttcata tgagtctaca tttgctgttg caacaggctt ataacctgct aataatagtt
    23581 cttcactact tcctgatata tcaactattt ttttaggatt tgatggtatc ttaacctctc
    23641 ctttaacaga ttgtacaact cttgtctctg atttatcttt atcctctgta ttcttatctg
    23701 aacatgctgt aacaagtact aacgtagtta taatagatat aaatattgca agtgatttta
    23761 tttttttcat gattacttct cctacttgtt tattatactt acatgatatt gattatcata
    23821 agcacatatt tattatagca tttttctatt tttagtcaat attattttgt aaaaggataa
    23881 aaattcctaa ttaatcttaa ttatatatag cttagataag gttttcttat attaaacagt
    23941 atttttttta agaattggac acaaacacaa tgacttttag gttaaacaat gataaaatgc
    24001 aatttataga taatgaattt agtaaggagg attcaaaaca tggtacattt agtatattgt
    24061 gataatgcag gaaaaaaagg agaaaaggtc ttagataaaa tcttggctgg aacaaaaaca
    24121 atggttatta gaggtgctgc tggtagaaaa ataccacata gtagagtatt tgaagatgag
    24181 ttactttatt ttatggaaaa aggaactgga aaaatatctg caaaagccac tgttaaaaaa
    24241 gtagaaaact atgttaaatt aacagaagat gaaatttcta aaattttaaa tgaaaatcaa
    24301 ccaaaattaa atttatctga aaagcaaaaa gttcgttggc ataaaaaatg tttatgctta
    24361 gttgaatttg aaaatgttga agaaataaca cctcttgatt ttgaccatca aggaaatatg
    24421 gatgattggc ttattgttga aaaaattgaa gatgtagttg ttggtactag tataccttac
    24481 aattatgaga agtctaaatt ttaaagtatc aataacataa tactatattg ctaaactcta
    24541 cttaaataaa aaaagagtgc atttaataat tataaaaatt atttttatac actctttttt
    24601 aataataaat ttaaattact tactttctaa tctttcatat acatctataa attcttctgc
    24661 taattgttga aaattcatag ctgtcattaa atggtcttgt gcatgaataa gtataacact
    24721 tatttcagtt ttattaccat tagcttcatt ttgtatcaat tctgtttgga atttatgagc
    24781 tttatgaatt gattctttcg acttttcaat acattctcta gctttatcta tattcccgct
    24841 ctttgcttct tttattgctt caaatgctag ccctttcgca tcacctgcaa atccaattat
    24901 tccaaaagat atctctatca tcttctcatc catttattcg acctcctaaa tatctctata
    24961 gcttgatatg actaatccat tcttttctaa aaattctttg acttttttag atgtaagtat
    25021 cttgtgctct ttcgttctat caatagcata agatgtagaa tttaaaatat aatcatctaa
    25081 aaaagctggg tgagacataa tatccactac atcatacttc attatttcgt ctatattttt
    25141 tataaaatat tcttcactta cattttcctt ataaaaacta tctattaaag gaactacttt
    25201 agaatactct aaattatatt caaatccacc tcttattgga agattatact tggttacaat
    25261 cttctctact actggcttta aacttactaa agtatggata tgatggtgac tatctaaatg
    25321 agatatatct attccaagcc cttttaccct atctatttga gcacaaagtt ctctataaat
    25381 ctcatcacaa tccatttttt caataatttc gtctgtaatc ctcctaataa aaaaaccgtc
    25441 tttatcaaca atagttttta agttagataa taatggtcta ccacaactta aagtcatatg
    25501 aactccacaa tttaaagttt tattttcttt aagtaagcct actccatgtt ctaccccagg
    25561 catatttgcc atcatagaag tagacctaac tataccatta ttatgagctg aaattattcc
    25621 atagttcaca gcttcacaat atccaaaatc atctgcattt attataattt tagtcatacc
    25681 ataacctact ttctatagac aactatctat taaatctagg aagataatct ttatgagctt
    25741 ccaacatttc gtctaatata gtttttgcta agtcatcaga tggaatcaat gggtttatac
    25801 aaagtgccaa aagagcagat tcataatcgc catatacagc tgctttagct accaatactt
    25861 caaatgattt gatttggctt actaatccat gcacagaatc tggtaaatat cctatactta
    25921 ttggttttgg accatttttg gttattacac tactcacttc taccgcttgt tcatctttaa
    25981 aatctcttat agaaccattg tttaatgtat taactacttg gatatctttt ttgtcattgt
    26041 atatagaact tattaaatta caagcagcat cactatagta agcccctcct cttttttcaa
    26101 gttgaggtgg ttttacatct aatttttcat ccttatataa ttcaaataat tgttcttcca
    26161 attctttaac tacttgacct ctagcttttc cctctttaaa ctctctcaac tcatcctcaa
    26221 gcatttcttt agttttatag tagtatctat gatatggaca aggtattgca cccaaagatt
    26281 ttacaaattc agcattaaaa tcaattgcct ttatattttg catacttata tctgctttga
    26341 caaacttatc aattgcttct tttgtaacat cttctccatc taaagataca tttagaccat
    26401 aaaccatatg attaagacct gcaaaatcca tacttattct gtcactttct acattaaata
    26461 gtttggcaac atcatttttt aaatgaattg gcacattgca tagacctata tatcttctaa
    26521 aattagtata tttgaataca gcctctgtta ttattcctgt tggatttgta aaattcacca
    26581 tccatgcatt tggacataat tcttccacat cttttattat atcaaatata actggtattg
    26641 tgcgaagggc tttaaataaa cctccagcac cattagtttc ctgccccata actccatgag
    26701 ataaaggaat agtttcatct tttattctcg catctaaaag acctactcta agttgtgttg
    26761 tcacaaagtc tgcatctctt agagcttctc ttctatctaa agtaagattt attttcatat
    26821 ctactccagc tttttttacc atcctcttag ctagatttcc aactatctct aatttttctt
    26881 ttccagcttc tatatctaca agccataatt cctttacagg taactcttca tatctattta
    26941 taaaaccctc tacaagctca ggagtataac ttgaacctcc tccaattgta actattttta
    27001 gtcccttttt catctcatta taccatcctt acctataatt taatatactc tacaaaaccc
    27061 tatacattta tttaccgaaa taaaactacc tagttgttaa ttttcaattt attagcttcc
    27121 attttttcag acgctatgac aaatggaata tatattaata ttgataatat taaattaaat
    27181 agtgctaaag ctccaccagt ccagtcacct gtagccatcg ctccacctaa tataggaggt
    27241 gttacccaag gtattttagg taaaattact ggtgctacta atccaaaatc tattgctaag
    27301 tatgatacag cactacatat cactggtgca agtacaaatg gaatcataaa tataggattt
    27361 aaaactattg gaagtccgaa tatcaatggt tctcctatat taaatattgc aggtggccca
    27421 cctaatacta tcatttcttt acgtcttctc ccagctatta ataaagctac tattaatccc
    27481 aataccattc cagaaccacc taaatataca aatgcatcaa agaatgcacc tgcaagtaca
    27541 tggtatcctt ccgttgctcc tgcttgagcc aaggcagcat tttctccacc taatttcata
    27601 agtatagttt ctgtaaaagg aagtgctata tttgcaccat gaagtccaac agtccaaagt
    27661 atatgaatta taaatgcaat aagcattgta gaacctagac tatcagctac atttgatagc
    27721 ggtgctccta aataagtatt taaaatatct gttaagaaac ttccattaga aagcattttt
    27781 ataaatattc ctattacagt aaatatcatt atagttaaca ttcctggtat taactttgca
    27841 aatgatcttg aaactgctgg tggaactcca tctggcattt ttatagttaa tttctttacc
    27901 ttagataatc ttacaaatat ctcagttgat aataatccta ttaatataga agagaataaa
    27961 gctgctgtac ctaagtaggt ttgttgtatc attccccatc cttcaactac tgtagttcct
    28021 ccctcaggaa ctattttacc aatttgtgga gccattataa aaaagataga taaagctata
    28081 agcccagatt gtaacccatc actctcataa gattttgcta gaaaataagc aacacctatt
    28141 actaagaata ctgccattgt acctattgct ccccaccaaa ggtcgcctcc aaatgtggtc
    28201 caattttctc caaatatact tgccatcaaa tttttgtatg cttccaatgg taagttattt
    28261 atcaatgttc ccaaggcacc taccatagtt ataggtatca ttactataaa tgcatctctt
    28321 actgctacta agtgcctttg agctcctatt ttagcagcta caggcactat atatttatcc
    28381 ataaatgaca tgaatttttc cattttacat cctccagtac aactttattt tgaactattt
    28441 aactctacag ctctatctaa cacagctttt ccattacatc ttccataatc catcatattt
    28501 ataacttcta ctggaatatt atatggtttt gccatttcag ttgcttttcc taatacatat
    28561 ctaacttgag gccctaaaag taagacatca caattttcta tttcattttt taagttcact
    28621 tctgatatag cccatatttt tgcttctata ccgttttcct ttgctgcatc ctccattttt
    28681 gtaactaata aactagttga cattcctgct gaacaagcta acattatttt tatcattttt
    28741 tcccctccaa attagattca ttaaatattt tttaagtcat aaatttttta aactatacat
    28801 ttaaaatatg cttttctatt aattttttct taatgtatat atcttatttt taaatgtata
    28861 tacatttaat tcttgatatt attatatacc aaatgtatat acatttcaag aaaacttttt
    28921 cattttattt aaattttatt cgacaacagc attcaaagtt aatgtatatc catagtgata
    28981 tgtgtgtgag tactcaaaaa tctcactatt ttgtaactta caaatttggc ttatatataa
    29041 aagtggctct ggttcttcta attttagatt atccatacat tcttcattcg atattacagc
    29101 ttgtacttct ctctctgaca tggctatctt gtatccacac acttcttcaa caaaattata
    29161 aagagaattt tcacaatgag tcttatttaa atttggaaat aacttaactg gcatataagt
    29221 aatcatatat gaaactcttg catcattggc taatcttact ctttttattt cccaaacatt
    29281 ttcttctaaa cttatattca acttacttga taaattttca tctgcctgaa ctatttcaag
    29341 agataccaat atattagata ctttatagcc tttactttcc atctctttag taaatccact
    29401 tacagacgat atattatcat atatattatt tgcaataact atactaccta cacctctttg
    29461 tctagttata tagccttctt taactagatt attaagtgca gaccttacag tcattctagt
    29521 tacattaaat tcttcactca gctgtttttc agaagggatt aaatcccctt ctttcaaact
    29581 gtctgaattt attaactctc taacatgatt ttctataact ttataaattg gctctttcat
    29641 ttaattcact ccttattaat taaatctttt atataataag ataattttat atacaaataa
    29701 agaaaatgtc tatacattta agataattta aattttattc agacatcttg tacaactttc
    29761 cgagttttaa taaagatata aatagactta tcattaatat accttttaat atacttgata
    29821 aggttatact ccaccataca ccatttagac ccaacatttc tggtctagat ataaagtaag
    29881 ccaatggtac tcttgcacca gtcagtatag taattataat agaaggtata taggttctgc
    29941 ctaatccttt gaaagctcct gttgttatta tttctaaaca cataaaaaat tgagaatagc
    30001 ctaatatttt aagataaatt gctccttttt ctattgcctc actttcatct ataaaaatag
    30061 aaaatatact ctctcctgca aatactaata ccaaagtagt tattatccca agtacagaag
    30121 atacagttat agctattttg caacctttat ttattctaga atacttcttg gcaccataat
    30181 tttgtccaac aaaactgcct aatgcaactg atagcccttc agccgtcatc caggaaattg
    30241 cttctatttg tgagcctact ttttgtactg caacagccac aggcccccat gatgctacta
    30301 ttactcctaa tagcattgag aatactgtaa acataccact ttgtattgct ataggtaatc
    30361 ccaatttata cagaacttta taatatttag tctctatttc tttaaatagt cttaatttaa
    30421 aatattcttc tttactttta actattatat atataaaaca tgtagtaact actatctgag
    30481 caaatacagt tgcaatagca gctcctgcaa caccaagttt tggagcaaat ccccatccaa
    30541 aaattaatat aggatcaagt ataatattag ttattaatcc tactgtatta atacgaaaag
    30601 gagttttact atttccaaga ccgttgaaaa ttgcagtaaa tactggatta ataaaataaa
    30661 ataccatacc tagagctact attatcaaat actgtctaga catggttata acttctaagt
    30721 ctcctaatct aaaaaaacct atcaattgtt tattaagtac tattaaagaa attgtatata
    30781 ttattgctag tataatattt atttctattg ctgacttcat atatgattta gtagtgctta
    30841 tattatttgc accaatactt tgtgctactt ttatctctcc acctactttt gaaatcataa
    30901 taaatgccat tgcaagccat ggaaaaaaac cagctgttcc aactgcagct actgctttac
    30961 ttccggcttt accaacccat atcatatcta tcatgttata ccccatctgt ataaatgaag
    31021 tccccattat tggaagtgat agcttaagta atttttctgt aattcttcct tctgttaaat
    31081 taattctgtc tttcattttc cacctctttt atattaaatt tatttgctta tatagctaga
    31141 ttaatcattt tatttgattg agttcactat ccttcaattt atattatcat atctcaagat
    31201 tgcatctcaa ccatacttat gtttaagata aagtatttta cctatctctg ataaaattaa
    31261 tacttatgaa tataaatata gtcatttcat caatacatat tataaccctt aacttaactt
    31321 caattaagta tacatactgt aatcaattag aatattttat aatgtatctt ccattaagaa
    31381 ataacaaata taagtgattt atctataatt ttatttatac ataaaatagt atactgcaat
    31441 aataaaaaaa ctatatttga atactttaag catgtttact aattgtaatt tccacacaaa
    31501 tttgataata tatgaatata ttatggtgaa atttatagat atatattaat tatggggaga
    31561 ttgtgggagg ttcttttcac aagacattta agctatgaaa ataaataaaa aatttaatag
    31621 agttgatatg caggtttcaa tacttactgc aattattgta ataatatctt cctcatgtgt
    31681 atattttttt aattatacat taacttataa gagcatgata tactctttaa gtgagaggtc
    31741 aaaacatatc tatgaatatg tagagagaaa tattgataaa aataccttta ctgaaattaa
    31801 ttctaaagaa gaccaatcaa aaaagtctta taaaagttct aaaatcctat tagaatctgt
    31861 aaaaaatact actggagtca tgtatctata tactgctaaa aaaacaaaag atggatcact
    31921 agtatatgtt gttgatggtt taaattcatc tagaagtgat tttagaaatg ctggtgattt
    31981 gatagaaaaa gagatttggg gagatcttaa caaagcatta gataatcata tattgttacc
    32041 taaaaagatt aaatcaacat cttggggata catatttata tcgtattttc ctattcacaa
    32101 tgatgttggt aaggttgttg gagtaatagg aattgaattt gaagcaaaac atcaatacaa
    32161 tacatttaaa tttataagta tagttacacc aataatagca ttattaactt gtctaatatc
    32221 agcattgatt gcagtgattc tatttaaaag aatatctaat cctacatata ctgattttgc
    32281 taatacagat tatttaacag gcctaaaaaa tagaaatgct tttgaaatag atatgaataa
    32341 cttaaacaat tcaaatctaa agaatctaat atctatcata tcaatagatt tagacggttt
    32401 aaaaaagatt aatgataaat ttggccatca gacaggagac ttatatatta agcaagcaag
    32461 taaaattata caagatgtaa taaataaaaa atatatagtt tatcgtattg gaggtgatga
    32521 atacataatt attctaaaaa atttatctca caaagaaata aatagtatta taaataatat
    32581 taatttaaaa atagttgaag aaaactcaac taatgaatta aaattatcaa tgtctattgg
    32641 atatgctatt tttgatgaaa aagtagatgt ttctttattt gatacatatc gtcgtgcaga
    32701 ttctcaaatg tatgataaca aaaagaaaat taataaataa tatcaataat aaatttaaaa
    32761 agtctattct ttaaataata aatcataaga atagactttt taaatttgtg tttttataag
    32821 ataaattatt taattaaagt ttgttctttt ttctaaacga ttctttataa attcatatat
    32881 tactttcact aatatatttg tgactaatat taacacagct agtgctgctg cctgtgcagt
    32941 actcccctta tcatctagtt gtacaattga tattgcagct acttttgaac ttgcagtata
    33001 tataaaaact agtgctgata ttgtcaccat agagtttaga aaaaagtaca taaagttctc
    33061 taaaactgct ggcaaacaaa gtggtatagt tacgttctta agaattgatg cgctgtttat
    33121 tcccattgat ttagctacca tgtccaattc tttatctact ttcttaatag cagtagtagc
    33181 tgttataaat gctactgaaa agaaatgaac tatattacat ataaccatta taagtaatgt
    33241 tccatataat ccattaaaca aatttttaat gtatattgta tccataaaat taaactctaa
    33301 tttattaaaa aataatacat aagctatacc taaaactaat cctggcaatg ccataggcaa
    33361 tgtacttaaa aaatacccaa atgattttaa atatggagct ttttctgttt tttcatttaa
    33421 atatgcaaat aagaaaacaa agctcgtccc tagaatagct gtcaatagtg acatttttat
    33481 tgaatttata aatggtttaa ttccatcaat actgtttttc atagaaaagt gttccagtgt
    33541 aagtcttaaa tcatatggcc aaactgatat taatgaggat aagattacag ttaacattac
    33601 agctattata ccaaatgcaa taattcctac aacccctcct aaaatactat ctcttaactt
    33661 attagtttta accttatatg gaattgactt tgaagttata tatgagtttt gatttttttc
    33721 aattttttta gttactataa acgatattat tgcaggtatt aaaagtaata ttgatacagt
    33781 tgctcccatt ggcatattct gttgccctat aatttgctta tatacatcta ttgataatac
    33841 attaaactct cctcctatca cctttggagc tccaaaatct gtaaaacata atgtaaatga
    33901 tacgaatgca gaacttatta aagagtattt tatacttggg attgttatgc ttataaattc
    33961 tcttatcttg cttatcccca tagtattact agcttcatat agtctataat cggttgaact
    34021 tagagatata taaagtattt gaaaggattg aggaaataca tatataattt gtgccaatat
    34081 tattcccact gctccatata ttggaacatg cactccaagt aaattagtta ccaaaccttg
    34141 atttccaaat aaatatacta gacctattgc atgcatcatt gttggagcaa acataggaaa
    34201 catagctaat gatttaaaaa aacctttaaa tttaatcttt gttctagtta atgcatatgc
    34261 atatactaat gcaagtgtta gtgatattac agttgatata actgatacaa atactgtatt
    34321 ttttagagaa tttaatagag attcagaagc caagtattct tgaaagtttt gtaatcctac
    34381 aaagactcca tctttatcta aaaaagcctg tacaaataga tatattatag gtgctaataa
    34441 aaatattact aaagacataa ctaatactgt aagcatacat ttttttatat ctaaattcat
    34501 ttttgattta agacttatac ttttagaact tatagcatta ttattcatac acttatgctc
    34561 ctttaacttt tggatacttt aaatagtatt tttcgtctat acttattttt acaaggtcac
    34621 cttctactaa atttaaatta acccactctt ttatagaaac atcacttatt atgaaatttt
    34681 catttaattt atcttttatt tcaactctca ataaattccc tctaaactct atatttgata
    34741 ttataccttg gtagttttct tttgtcgatt tctctatttg aacatattct ggtcttacag
    34801 ttaatataga atctccatta tcaaaacagt ttgtatctcc tataaattga gctgtaaata
    34861 ggttttgagg attttggtat atttcttctg gagtccctat ttgcattata tctcctccat
    34921 ttagaatagc tattttatct gccatagtta tagcttcttc ttggtcatgt gttaccatta
    34981 ttgtcgttat attaagttct ttttgtaatc ttttaatgtc cattctaagc ttgtgtctaa
    35041 cctttgcatc taatgcggac attggttcat ctaaaagtaa aaactttggt tcaagagcta
    35101 atgctcttgc tattgcaatc ctctgttgtt gcccacctga taatgcttta ggatatttat
    35161 gagcttcatt agtcaatcca actgtttcaa gtacttcctt aactttatta tctatttttt
    35221 cttttgaaac ttttctctct tttagtggaa aagctatatt gttataagca gtcatattcg
    35281 gaaataatgc atatgattga aatactatac caaaacctct ctttgatggc tctaaatttg
    35341 taatatcttt atcctggaga attatagttc cactatttac atcttcaaga cctgctatta
    35401 ttctaagtaa tgtagtttta ccacatccac taggtccgag taaacataga aattcccctt
    35461 cttcaatatc caaactaata ttatttaaaa cccttttttg gtcataactt ttaaacacat
    35521 tatttatttt taagtaactc atatcttatc tctccttata tagttatttt tttattttaa
    35581 attttatatc ctactttttc ttctaaaatt ctttcatcta aattattaat ttaaacctta
    35641 cttactttca gattttgaac cgtattttga ttcccaagtt ttaagtactg tacttctgtt
    35701 tttggcagca gagtttaaat caacttcttt tataagctgt tcaaatggtt gtttagaata
    35761 accttttgga actggattat atgtctttat ggaagtaact gctacttcat gtgcatatct
    35821 atccatagct gaatcactta ttgcccaatc taaaaatagc tttgcttctt tttttatatt
    35881 atctttttta actaaggcat ttgcttctaa gtcccaacct gaaccttcct ttggaaatac
    35941 aacctctata ggttctccac tagataattg ttgtgtagct ctatatccaa gtgtaatacc
    36001 tattggatac tctccagagc cagctaactt cgcaggtttt gaacctgagt gtgtatatgt
    36061 agctatattt ttatgtaatt tatccatgta attgtaagca tctttttcac ccatcatttg
    36121 aataagccct gatactgtaa gataccccgt tccagatgat gctggatttg gcattgttat
    36181 aagtccttta tattctggtt ttattaaatc ttcataactt tttggaatat ctatacctag
    36241 tttcttaagt tccttataat taactactat tgctgtctct gggacactca ttcctaccca
    36301 agatacatca tcttcagtat ctttaaattt tttatcaact ttatcataat ttttaggctt
    36361 atatgcttca agtgcaccat ctttttttag ctctatcatg ttcattgctg ataatcccca
    36421 tataatatct gcttgtggat tatctttttc tgctaatagt tttgaagcta ttacaccatg
    36481 agaatccata actatctcta catctatatt gggatactct tctctaaaac cttccatata
    36541 ataagctatc tgctcttcct ctagtgctgc atatattgta agtttattat ttccttttcc
    36601 ttgagtagtt tgtggtgttg ttgatatgca acttgtcata aatgtaatca ctacaatgca
    36661 cagacaagta aatataaatt ctttcatatc ttcccccttt gtgttttttc ctttttttat
    36721 ttgtttttta ttgtttttgc ttatgtttat ataatacacc taatttgtta atataaagtt
    36781 aagtgtaaat taacagttcg ttaattttta gaaatttttg ttaaatatat tatttttagt
    36841 taaatacagt ctgtttttaa acaatatatt aatttttata gtatctacat tttaatttaa
    36901 acataaaaaa taagtctaac ctctataaag tagaatgtta gacttatttt tataagtatt
    36961 taattataca accaaatcat tttttttctt ttcttcaagt gcttttttaa gcaccatatc
    37021 cttaaccttc ataagtctag tagtacttgc tctctcattt tcttcaagct tcatagctat
    37081 atacttaata gtctcaatgt aatttggtat cattacattt tcaagagcat taacacgtct
    37141 acgtgtcttt tctatctctt gtgcaagaag ctgtgcactt ttttcacttt ctgcaagtct
    37201 taaaagtggt tgcatagcat ctgagaaagc ttccatagca gaatctaatt cacctgatgt
    37261 aaaagcaaga ccataaggat aaatatcact ttgattgttt tcagttttaa agtcaaacac
    37321 aggaacatca acactcatta tatttctcgt agaaacatct actgaaactg attgctttgg
    37381 catcataagt gctgaaccca gatactcctg actcataact gctcttgcga ttataaagtt
    37441 tttataagca gtatcaagag cattttctgc ttcttcacgc aatcgcttat tttctcttac
    37501 aatctcaaga aattgtttca taagttcatc aagtttatct ttaagaagct tatgacctct
    37561 tgtagcagtt ttaaggagtt tcttaagtct agtcatttcc atacgtgttg gatttatatt
    37621 taatctagcc acagatatca ctccttaggc atatattttt caaaatattc atcacggata
    37681 cgtttaagtt cactttttgg taacatacta agaagcttcc atccgatttc taatgtctgc
    37741 tcaatagttc tatcagttct aaatccttgt gaaacatatt ctttctcaaa ttcatctgca
    37801 aacttagcat acattaaatc aatctcagaa agtgcagatt caccaagtat agccataagt
    37861 tcttttgcat ccttacctct tgaataagct gcaaaaagct ggttcattgt atctgcatgg
    37921 tcttcacgag tcttaccttt accaatacct ttatctttta gacgagaaag tgatggtaaa
    37981 acatctatag gaggctgtat atctttttta taaagttcac ggctaagtat aatttgccct
    38041 tctgtaatgt atcctgtaag gtcaggaatt ggatgtgtct tatcatcctc aggcattgta
    38101 agtattggaa tcatagttat agacccttca tgccctttca tttttcctgc tctttcatac
    38161 atagtagcaa ggtcagtata aagatatcct ggatatccac gacgtcctgg aacttctttt
    38221 ttagctgctg aaacttcacg caatgcctct gcatagtttg taatatccgt tattataaca
    38281 agtacatgca tatcttgctc aaaagcaagg tattctgctg ctgtaagtgc cattctaggt
    38341 gtagatacac gctctacagc tgggtcattt gcaaggttta caaacactac agaacggtcg
    38401 attgcaccag ttgctttaaa atcgcttata aaaaattcag catcttcaaa agttatacca
    38461 actgcagcaa atacaacggc gaacttagaa tcagttccac gtacttttgc ctgtcttgca
    38521 atctgaactg caagattggc atgtggcaaa ccagaccctg agaatatagg aagtttttgc
    38581 cctctaacca atgtattaag tccatctata gcagagactc cagtttgtat aaattcggcc
    38641 ggataatcac gtgctgctgg atttattgga gctccattta tatcaagtct tttatctggt
    38701 ataatttctg gacctccatc aataggtcta cccattccac taaaaacacg gcctaatata
    38761 tctggtgaaa ctccaaagtc aagactcttt cctaaaaaac gaactttact ttcagcaagg
    38821 tttatacctg ttgagctttc gaaaagctga acaagtgcag tatctccatt tacctctaga
    38881 actttgcaac ggcgaatttc tccatttgaa agttcaattt ctccaagttc atcaaatttt
    38941 acaccttcta cgccgtttat taacataaga ggaccggcta cctcttgtat tgacttatat
    39001 tcctttatca tagttcttca gcccccctct ttttaataag gtctgcaagc tcgttgtcta
    39061 tttcatcttc aattgaatca taagctgcat ctatatcgcc ttcttctaca tacttagcac
    39121 gaccaatctt ttcaagaact gaaagcttga taatatcgtt tatagtaaca ttttgcttga
    39181 tagcatcttg tgctgactta tagaatttta atataagtcc catcattcta tattgtttat
    39241 gaagtgatgt ataagtatca acttcatgga aagagttttg atgaaggaag tcttctctta
    39301 tagaacgagt aacctctagt atcaatctat ctccatctga aagtgaatca accccaacca
    39361 attgaactat ttcttgaagt tcagattcaa cttgtaaaag tttcatagct tgagctcttt
    39421 gagctgagaa attatcattt acatttttat ctgcccatac atcaactttt tcctgataga
    39481 gtgaataact agtcaaccag ttaattgctg ggaaatgacg tttatatgct aaagatgcat
    39541 caagacccca gaatactttt actatacgaa gcgttgcttg gctgacaggt tctgatgtat
    39601 caccaccagg aggagaaact gcaccaattg ctgtaagtgt tccttctctt tcatccatac
    39661 ctagacaatt tacctttcct gctctctcat agaattgagc aagacgtgaa cctaagtatg
    39721 caggataacc ttcttcacca ggcatctcct ctaaacgacc actcatctct ctaagagcct
    39781 cagcccatct tgatgtagag tctgccataa gtgcaacact atatcccata tctctaaaat
    39841 attcagctat tgtaatacca gtgtatatag aagcttcacg tgcagcaact ggcatatctg
    39901 atgtatttgc tataagcaca gttctttgca taagtgactc gcctgttctt gggtctttta
    39961 attcaggaaa ttcaagaaga acatctgtca tttcatttcc acgctcgcca cagcctatat
    40021 atacaactat atctgcatct gcccatttag caagctgatg ctgtgtaaca gtttttccac
    40081 ttccaaaagg tcccggtata gctgctaccc caccttttgt gattgggaaa aatgtatcta
    40141 taactctttg acctgtaaca agaagtgaat ctggtgtttt cttttctcta tatggtcttt
    40201 cttttctaac tggccatttt tgcatcatag ttacaggtat atcattacct ttttcatctg
    40261 taataacaca aactgtatct tcaacagtaa attcaccaga atatatttct tttatagtac
    40321 ccttaactcc ataaggaacc ataattttac attctactac tgctgtttct tgaacgccac
    40381 caattatatc tcctgctaca accttatcac caacagattt tttaggttta aattcccact
    40441 tagttgttct atcaagtgaa gatacttcaa cacctttagt tatattagta cctgaaatat
    40501 catacatttt ttcaagtgga cgttgaatac catcataaat agttgaaata agacctggtc
    40561 caagttcaac actcataggc attccaagag aaactacttc ttcccctggt cctagaccgg
    40621 aagtttcctc ataaacctgt atagaagctt tatctccatg catttctata atctcgccta
    40681 taagatgttt atctgaaact cttacgacgt caaacatatt cgcatctctc atgccctccg
    40741 ctacaacaag cggtcctgac acctttacaa ttgtgcctgt tttcatattc tctcacctgc
    40801 aatcatatac tttatcaaaa tttaaatatt ccctcctata ttttattagc acatatcttt
    40861 agcaccaata aaatttgaga ttgtctttta ttcaactttc atctattctt taaataatat
    40921 atctgcacct atagcacgtt tactagattc tcttacttca ttcataccta atcccatact
    40981 tcctccaata cctggaataa ctataatggc aggaagttgg taatctttgt attttgctat
    41041 tgattctttt gcaagcaatg atgcctgttc tgtaatataa attatcgcat attcatcttc
    41101 aaccaactta tgtatacttt ttttaatttc gtcactatca taggctggga agatatctat
    41161 tccaagagct aaaaatccca tgatgctatc tttatctcca actactccta ccttatacat
    41221 aagcatccct tagcctttct tttatagtat ctgcgtctat attattaagc ttactagtca
    41281 aaataattct aattgttttt atctctgatt ctttagcata taaatatgca attagtggtt
    41341 ctaatgtaag aggcttaaat tttgctcctc taacatattc cataagatag tcatcacaaa
    41401 gtttttcaaa gacagtaaaa ccactatcca tgccattttt acagacatca gagtaagaag
    41461 tagatttaaa acatgatgct ggagtatctg aggaaaaagc ttctaagaat ttttccttat
    41521 caagtgagcc acctgaaaca aacacattca taaacatatc aaaatctttc ttcatatttt
    41581 tgactcttat aaaactcttt agatttgtta aatcgcaaac tttcataaca taatctatta
    41641 caaatttgtt ttggctttct ttagcagttt ctttcatact ggtaaaggtt gctttatcta
    41701 gaacaatatc aaccatctgg ccattttgtg tcttattata aacatcaaat gcctctaata
    41761 tagctgatga cattatctta ggtaaatcac taaaactacg atttgctaaa gattctctaa
    41821 gcttaataag aggaattgta cctccatcaa ttaaatattt ctcaccatct acaccagaaa
    41881 tttcttcttt aatcagcact tttaaattgt gatagtcatt tttataaaga aatacatcaa
    41941 caaatttttc ttcaggagct agacttttaa gaatttcata tgtctttgat aattcttgag
    42001 tcaaaatatt ctcaaaatta tgtatattat tatttgatgt atcagcataa cctgcctcag
    42061 ctataattct taaagagtct tcagggcttt tggcttctgc catttgaata aacatttgtc
    42121 tgttaagaag ctgtttttca agcactctta ttcgtgcaac ggcataggga taagtttttt
    42181 cttctgccat tttttaaccc ctccttaatt aaacaaaatt tctgctgcaa tttgttcaat
    42241 atattcatgc tctactgcaa ttatggcttc aaatgaataa ttgtactcaa tgtcaccttt
    42301 tttaactatg aaacctccag taatatctct agtttcatca gacactttaa tgcctttttt
    42361 tgatagcaca tcttttaatg tttttttatc ttttttagat aatactatct ctgaatcatc
    42421 tgtacaattt gatttttcaa tcatgtttag tataatctct tcatattcat tatctttaag
    42481 atttaaaagt ttgtttttag cttctaaaat tacctcttct aaaatctctt gtttttttga
    42541 taatatttgc tttttagcct ccatataagc tcctgatatt tccttagaag ccgctttttc
    42601 tgcttctgcc tctgcaagct ttgtatagga agccatttcc ttttcagctt tttcattagc
    42661 tgaatttact tttaaatctg cttcactttt agctttatcc aatatttctt gtgcttcttg
    42721 tttggcatca gctataattc tgtcaatcat tttttgttca ttacccatag gagtgcttcc
    42781 tccttttcat tttttttact tatgaattat aatttataat taaccaattg caattccatt
    42841 gtaagctaag aaagaaacaa gtagtcctaa aagagcatat gtttcaacta ttgctgcaaa
    42901 tgttatagct ttaccaagtt cttcaggtct ttttgcaact attccaactc ctgctgctgc
    42961 tgctttacct tgtgctatac ctgatactaa accaactaaa ccaattggaa gtcctgccat
    43021 aagaagttgt aaaccttgag ctgatgttgg tattgcatct ccaccaataa tccctgcttt
    43081 tgataaaata aggaaagcta tgataaatcc atacagacct tgtgtaccag gaagaagttg
    43141 taaaactaga agttgaccga atttactagg gtcttctgtt acaacacctg ctgcggcttg
    43201 acctgctatt gaagtacctt tagcagaacc catacctgca aataatgctg ctatagcagc
    43261 tcctgatatt gccaaaaatt gaccatttcc taacgcgttt aaaaattcca tatttacatc
    43321 tcctttttat taatgcttgt atattttgtt ttatacttaa acggaacaaa aggaactcct
    43381 ccgccttcat aaaacttatt gaaaaattct acatactgta acctacttgt atgaacatat
    43441 gcacctagtg catttataag aaggtttatt gtgtgtccta caacaccgat tacaactgca
    43501 agtatcggac cagctatagc ccctaaaagg ttgataactt gtgctataac tcctgttgtt
    43561 aaacaaagcg ccataattct tgtataagat aatatatcag caaaataact tgttatgcca
    43621 tagagacttg aaaatccttt aaaaagctta acaggaatac ctttaaagct tcttccttga
    43681 gtaagtacaa gacctatagc acctataatt gctaaatatt ttcctacttc tgaaaataca
    43741 cctatgtccc ctgcaacaaa aggtattata agtagacata cacctgttat acacaaatac
    43801 caaaaaccta tgtcaaagat tgcatctatt acctttccac ttcttattaa tgtatatgct
    43861 tttattccca aacctacata tatatggata ataccgaaca gtaacgacat tcccataagt
    43921 gtcataacat cctctaaagg attaattaat gcttttatag gaattaaatc ccccaaaata
    43981 cccccgaata taagacccca tacagtagtt gaaactccac agataccaat aagtttaatt
    44041 aaatttcctt cacctttttg tatctttaat ttatatacaa caaatccaca gactactgca
    44101 attattaacc catatgcggc gtcacttagc atcattccaa agaatacaac aaagaagaaa
    44161 gttaaaattg tatttgggtc aatatctttt gtacttggat agctgtacat gttagttaca
    44221 ctttcaaaag gtgttactaa tttattgttt tcaagcagaa ttgggaagcc ttcttcttta
    44281 cttccttctt cagtctgaac acaacaatca aacttttctg taatttcttt aattaattca
    44341 tctgcccttt tgcttggtaa ccaaccatta agacagaatg ttgtttttgt tttgacaagt
    44401 ctctctataa tctttttttg gtctctttct atagtgtaaa aatcatatag gttttcaagc
    44461 atgtgtatac tatctgcatg tcttttaatt tcctctgttt tgtccttaga aacgttttcc
    44521 tgcctaatta tcatttcatt gtatttcttg atagcttgta acggtgtacc ttcttcattt
    44581 ggtaatgaaa ccactgaaaa attaaattct tttaaaactt ctaaaacatc atcaaaagtt
    44641 tcttttaaag taattaaata aacatagttc attaacttgt cagaattgac aatcccaata
    44701 acactttctg gtatttcatc ttctaatttt actcttaaag catcaatatt tgcgcttatg
    44761 ggcacagttc caagtatagt ttttgtatac ttagtttcca tattttcaag aggaatatca
    44821 aagttcaccc atggttctaa catactttta ttgttgttaa gtaagtttat gttagacctt
    44881 attgtaccta tctccttgtg caaattgttt aactccagag cttcagcata aacttgccct
    44941 tctttttcac tatcaatttt gttaaactct ctttttggtg caagtaggga ttttttttgt
    45001 ttcactagtg aagaaacaat attaattgct tggtttacat gagacatttt ctgctctagc
    45061 attataactt cagattcttc actatctttt acaagataat tttttaattc ctcctcctca
    45121 gtaagaaagg cactatcatc aatctgaaca aacccacggt tcataagtag ctttaaaatc
    45181 tggctttttt gagcttctaa gccaacaata gatattttgt tcatattaac tattgccata
    45241 ttatctcact acctttccaa taaccgcttc aatagcctta ctttttcttt cttcagcagt
    45301 tttttcaatt ttcagacatt cacttttagc attagctaag attttatcaa cctctgctct
    45361 actgtcggtg actgccttct caataagagt ttcttccata agctttgctt gttttcttac
    45421 atcagcaaat atgctttcac atttgtttac agcatcagct ctgatagact ctgcatcagt
    45481 aacagctttt actttgatac tgtctgctgt ctgctctgcc tcaacaatgc tttttattat
    45541 ttcaaaagcc atccaaatca cctctcttac ctttttacta tgtatataac caattagatt
    45601 tttttattct aattagatta ctacctcatt gttaaagtat aaacagcatt tttatataat
    45661 aattaatatt tttcaacgtt tatttcttaa taaaacatat tttagaatag tacttgccta
    45721 gatttacatc cttttttgac ttacttttag actatgacat gagtatggtt tttaaatacc
    45781 attattttca ataattataa tttaaagcga tatagtttgt caatagcgtt attttgtttt
    45841 aaaattaacg ctatgtcgac attttaaatt tttattttat tttttttata attttaattt
    45901 aatttattac atataaaagt tttttatatt ctttttcaac ctctaaccct tgaagtttat
    45961 tgtttttttt gaatttttag aaatatctcg cactatttta tctaactttt ataaatcatc
    46021 aagctatatc gatttttgtc accttatttt caatctaaat tttttatata tagttagaaa
    46081 taaatcaata tttttaaaga ttgataaaac ttacacttag ttctaatagt tttattttaa
    46141 ggcaatgtat taatcttaag tagataatat cattagttac tctatatatt cgacaacatt
    46201 ataaaaaaat cctcgtacca atcactccaa tcttcaatag gatatgctgc tcctccattt
    46261 acacttttca gtaggtttga tgtatattct tctcccatgg aaaaaagttc ccctactgtt
    46321 atatcataac ctctagatgt aacaacctta caaaatgaat ccataacatc acgttgttct
    46381 cttgttttta taagttcctt tctaaattca tcatcattct tagcttcttc taaaatttta
    46441 gttaattttt cattcattat gtatttctcc ttttaagtag tctttctgta aacttcaata
    46501 agctttatat aaaaaggaag ccatcctaaa taaactaaga tgacttcgct taactgttag
    46561 caacacattt actagtataa aataattggt gtacctggtt ctatattttc atatattttc
    46621 tttgccttag aaaaaggtgt atttacacat ccatgtgaac ctgaagactt atatatacct
    46681 ccaccaaaag aatttctcca agtagcatca tgaatgccta tgttcccatt aaatggaagc
    46741 caataactta caggtgaact gtaaccttga ccagttaatg aaacatttct agccttataa
    46801 ttaagaggat atatacctga tggtgtagca aatcctctag ctaaatcgcc tgttacaaca
    46861 tctgttgata caagcatttc tccattttta aagaaccaca tatgttggcc agaaagacta
    46921 atctctacat atgtatttcc aatatcatct ttattacgag atacagcttt ttgtatgtaa
    46981 ataggttctc ttgtaacatt tttaccagct tttacaacat caactaatgc ttcagcttct
    47041 ttctttttat ctatcaacca accataaatt ccacctgaaa ttgtaatatc tttacctgtg
    47101 gcagttttaa atggtcttga gtcaccaaaa gtactgtatt ttttacttaa acttcttgta
    47161 tattctacca tagcatcatt atcaaaagtt atgtcataat tcttttctga gtctactttt
    47221 aaccatttat taatttcaga accatctaaa gtttccttac ggtcactaaa atcatatgta
    47281 acgactacac caacatactt atctaatgtc ttaatagctt gtttcaactt ttctgaatcc
    47341 tttgtgttgt ttggtgcttc ataaacttta ctttcatcca gagaaataga agtattccca
    47401 gaattaattg agttttgtac taacttataa aaatcatctt ttttcacttt gtttccaagt
    47461 acctctggta ctataacata agcgttttta gtgctatcat acttaggata agcactctta
    47521 ggttctacaa catttttact atcaaaacaa gataaagcat taaacctttt ttctagtaaa
    47581 tctttattat atgaaagagt tatcatatta ttatactctt tagatttaaa tattccacca
    47641 atccatccaa tagcgctttg actatctttt atcttttgaa ctttatcatc tggtgtaaat
    47701 tttaaatcaa tatcttttgc ttttatttgt tctttcttat tgtttctttc ctctaagttt
    47761 aaagtatagg aattagtact agatgttaac aaatcattta cttgctcaac tgtttttcct
    47821 gaagccttta cgccattaat agtcgttcca aagtaaaaat gatttctaaa aaagattgcc
    47881 attcctatat aagctacaaa caaaacacct acaactatag ctatcttaaa ttttagattt
    47941 ttttgtccac tttcttgttt tacctctttt ccatcaatca taattaaatc cttccttaca
    48001 ttgtaattac ctatgtactt tattggtttt taaacaaaac catgtatgcc ttattttata
    48061 tacccaaatt actgtatcat tgtttatatt tttaaatata tagttatttc cataaaacca
    48121 ttttatcttt tagacaaaat gactattata aatcacccct ttcaaaatcc cttaaataca
    48181 atctctgcta tattaatata ttaccatact tttaataatc gtgggttatc tatctgtttt
    48241 tgtaacacta aactactctt ttaaaaaaga aactaatttt atgttagttt ctttatctta
    48301 acttattttt atattgaatt ttagcttaac ataacgttgt tatatagatt ctatttaatt
    48361 attttagttt ctatatcttt tccatcagca tattctttaa gtaactcctt agcatatcca
    48421 aaatattctt caatatcaga ttcttgacta aaagaaaata tacaaattaa tatttcattt
    48481 gcatcttctg ttatcattgc tctttcagaa tcactagttg gactcccaaa tttacccact
    48541 gaatcagaca atataggaag attttctata tttattaatt ctttcccaat acccttatac
    48601 tgttctcctt catctcctac agtaaaacat actggtgaat gtaatttgct tacattatat
    48661 gtacctactg gatataatga ttttattgat attaaattgt ttatttctac tatattattt
    48721 attgtataaa gacccttccc ttgaataatt cttctaacca atgcttctga agatgttcta
    48781 tatctacttg gattttttcc aagctcttta taagcatttc ttgcatcttt aattttatct
    48841 agtttcgtaa tgtcttcaac ttttagactt tgatttatag tatcacaaaa ctcatttatt
    48901 tcattaatta atttttctga gcttttattt actgctactt ttgcttgaat gcaacccaac
    48961 ttagcattag gccatctttc cttaagtttg tcatcaatac tcacatctat catacttaaa
    49021 caactcctat acactaaatt tttattaaag tttttatcat ctatacatta tagtactatt
    49081 cttttgttaa taacatgttt attagcaaaa taaatttatt tttaatataa tttttataaa
    49141 atacaattat tttttgcttt atattaaaca atacattgat aaaataaaaa aacactaatt
    49201 atttagcagt gatttattaa aatcaaacat tcataatagt gctttatata aaaattaaat
    49261 tttgttttaa agtaaatcta aaaaattctt agctggcatt ggtcgtgcaa aataaaatcc
    49321 ttgaactaaa tcacatccaa attgtttcat taactcagct tgttctttac tctcaacacc
    49381 ttctataatt acaactaaat ctagtgattt tgctagagac actattgatt ttgctatatt
    49441 gcgacttctc tcatcatgtt caataccatt aagaaaagac ttatctagtt tcaatatatc
    49501 aattggcata tctcttaaac aactaataga agaatatcct gtaccaaagt catccataga
    49561 aatagaaaat ccatacttcc tcaactcatt taaaacattc tttaaaatat ttaactcatt
    49621 aatagccaca gtttctgtta tctctatttc aattaaatct gaacttagat tatacatttg
    49681 tattgtttta ttgataaatt ttacaatatc tggttgatac aactcaactc tagataaatt
    49741 tattgaaact ggaactatct ttttaccttg ctttttccat tcagataaat cattgcaaac
    49801 cctttcaaat acaaatctcc caatttttaa aatcaactta cttttctctg ctattggaat
    49861 aaatactgcg ggacttataa aaccatgttc tggatgattc catcttatta gtgcttcact
    49921 ccctatcatt tctccagtta caagtgaaaa cttaggttga taatacactt caaattgatt
    49981 ttttaccaat gctattttta tatcatctaa aatcattgat tcttcactaa gcttattcct
    50041 aacatcttca ttataaatag cataatttat atttttacct ttagcaacag acctagccat
    50101 atttgcttta tttacagcct tttggatatc tacttcacct tcttcaacaa aataaatacc
    50161 cacacacaga gaaatttcaa atactatact caaattactt tcaatattat ttcgtatact
    50221 atcaattttt cttatacgtt tattttttcc atttttataa tcacagaaaa ttgcaaaata
    50281 atcacttgaa agtcgtgcat atacttcacc ttctgttaat tcttctttta aagcttttgc
    50341 tatcgtaatt aataaattgt caccaaattc atatccaaaa gtatcattaa tcatcttaaa
    50401 gttattaata tcaaaatata ctataatata atttaaagat ggtttatcta aaagatactt
    50461 actacactct ctataaaatt tattttgatt tcctatatgg gtaactttat cttcataagc
    50521 caaacttaat atttcttttt cattggattt ttttatataa attatataaa aaataattgc
    50581 aataaatatt aatataataa ataacactgc atatatagct ctgttgatta tttgttctga
    50641 attactaaat acaacagttt ctggaattga aaaaatcaaa ttccaatctt ttatataatt
    50701 attttcacta ttcccattat ccatagtagc atatcctaaa tatcttcttt cttccaacat
    50761 atttaataca gttgcacctg ttttattttc ttgcaattct ttttttaatc tatttatttc
    50821 tcgaatatca ttatcctgtt ttaacaattt ataaatattt tttgataggc tatcctcttg
    50881 cggatgaagg ataattgtac catcatttct tacaacataa gatatactat tattttcata
    50941 aaaagataca ttaataagtt tcatcagagt attgatattg taagtacaat ataaaactcc
    51001 aactacttta tcattattta ataatggtac tgaaatagta atcttcttta tagacttatt
    51061 tacttcatca acatatggac ttgaaacata cttcttacct ttaattgagt ttttgaaaaa
    51121 ttctctatct ttcactgaaa acttatcacc tttggtattt tcagcattac catttatatc
    51181 aactattgcc atagtgtaaa atttttgatt ttccatttca tctaaaagat ttttcatttt
    51241 ttcctcatta ggtatatctt gatttaaagc ataatagtta gaaaaaatat ttaaaacatc
    51301 taaatctttt tcaatttgtc tttgtgctag ttgagagcct tgtactgcaa cttcttttat
    51361 atgtattttt gcctctgaca taagaagatg ttttgtatct tctatataga tataccccat
    51421 tgtcaaaaat aatgatgtta aaaaaagaat tgtaattaat gtaaaacttt tatttctccc
    51481 tgtcattcga ctcctcccct aattagtcaa tacttcatat agataatata tcctttttat
    51541 accgattcat catttatatt gtatataact attcttaaat gttacacata aaatcaacct
    51601 aataaccata aaaacattct tttattatca taataacact atttagttga attttaaatt
    51661 aaagaataaa aactatcaat ttttctaaac aaaaaaactc ttatacaata aaactttgtt
    51721 ttatgtataa gagtttttat tttatctatt ttatagtaaa tttatcttcc ataatatgaa
    51781 tttaaataca tatcttttat ttcattcata agtggatatc ttggatttgc tcctgtacat
    51841 tggtcatcaa atgcttgaat caccatttca tctattgaat ccataaactt aggttcttca
    51901 actccaaact cttttataga tttaggaata ccaactttaa gttttaaatc ttcaatagct
    51961 accaataaac tttggaattt ctcttcatca gaatttccct ttaatcctaa aaatgaagct
    52021 atttcagcat atctttttaa tgcttctgga tatttatatt gagagaatgc tcccattttc
    52081 tttggtgcat ctgaagaatt aaatttcata acttctgtta tcaaaagagc atttgcaaca
    52141 ccatgtggta catggtggaa agcacctaat ttatgagcca tagagtgaca aacacccaaa
    52201 aatgcatttg caaaagccat acctgccatt gtagaagcat ttgccatttt ttctctagct
    52261 tctgggtctt ttgcaccatt atcatatgca cgtggaagat attcaaatat actcttaatg
    52321 gcttgtaaag ctaatccatt tgtataatca gttgcaagca tagatacata tgcttctaat
    52381 gcatgagtta atgcatctat accagaagca gatgttaagc tttttggcat attcatcatc
    52441 atatcagaat caactatagc catatttggc atcagttcat aatctgctaa tggatatttt
    52501 acccctgtat cttggtcagt tataacagca aatggtgtta cttctgaacc agtacctgct
    52561 gatgttggta tagctacaaa attagctttt tcacccattt tagggaatgt atataccctt
    52621 tttcttatat ccataaatct catagctagg tcttggaaat ctacatctgg atgctcatac
    52681 aatgtccaca ttatctttcc tgcatccatt gcacttcctc cacctataga tattattaca
    52741 tctggattaa attctctcat agctttagcg cctatcttag cacattccaa agttgggtct
    52801 ggttctactt caaagaatgt agtgtgctta atattcattt catctaataa atcagtaact
    52861 gctttagtat atccattatt gtaaaggaat gtatctgtta caataaaagc tctcttctta
    52921 ttcattacat ctttaagttc ttttaatgct acccctaaac aacctttttt gaaataaact
    52981 tttcctggtg atctaaacca aagcatattt tctctcctct cagctactgt tttaatatta
    53041 agtaaatgct taactcctac attttcagat actgaatttc caccccaaga cccacatcct
    53101 aaagtaagag aaggagctag tttaaaatta tataagtccc ctataccacc ttgagaagaa
    53161 ggagtattaa ttaaaattct acaagttttc atggctgaag taaacttatc aagtttttct
    53221 ctttgattta catcatcaat atataatgaa gatgtatgcc caaatccacc atcttctact
    53281 aatttttctg cttttctaac tgcattatca aaatcactgg ccttgtacat tgcaagtact
    53341 ggtgaaagtt tctcatgagc aaaagcttct tctatttcaa ctgattctac ttcacctatt
    53401 aaaaccttag tatctactgg gacttcaaag cctgcaagtt ttgctatagt acaagctgtt
    53461 tgcccaacta ttttagaatt taatcctcca tttactaaaa ttatatttct tattttttct
    53521 gtttcatctt tatctaataa ataagcacca cgctctttaa attctttttt gacttcattg
    53581 tatatatttt ctaatactat aaccgattgt tcagaagcac atatcatacc attgtcaaaa
    53641 gtcttagata caagaattga gtttacagca gtttttatat ctgcactctc atcaattatt
    53701 gctggagtat tacctgcacc aactccaata gctggttttc cagatgaata tgctgattta
    53761 accattccag gacctcctgt agcaagggtc aaatccactt ctttcataac agttgtagtt
    53821 aattctaaag atggctcatc tatccatcct attatgcctt caggagctcc agctaaaaca
    53881 gcagcctcaa gaacaacttt tgcagcctct atagtagaat tttttgctct tggatgcgga
    53941 gagaaaatta ttccatttct agtttttaaa gctaaaagtg ctttaaagat agcagtagaa
    54001 gttggattag ttgtaggtac aactgctgca ataacaccta ttggctcagc aattttagtc
    54061 ataccaaatg cttcgtcttt ctctataaca ccacaagttt tagtttcttt atatgcatta
    54121 tatatatact ctgaggcata atgattcttt ataaccttat cttcagcaat tcccatacca
    54181 gtttcctttt gtgccattat agctagagga actctttgct tattagctgc caaagatgct
    54241 gctaaaaaaa ttttatctac ctgctcttga gtatatgtag caaatatttt ttgagcttct
    54301 ctgatttttg ctagtttttt aactaagctt tcaacgttat ttacagtatc taaaccaact
    54361 gacataactt cattttcttt tttattttct attattactt gttctttatg agccatttta
    54421 acaacctcct gagatagttt tttatttatt atgttaattt attaacatta tagcatttac
    54481 tttttctatt ttcaagttat ttttttaaca ttttattttt tatctaatat aacatcattt
    54541 ctactaataa tatctagctt tacaaatact taatttaact agatatcact ttatttttta
    54601 cgcaaatcac taataaaatc atctaaaaac ttaaggataa cgttattttt ttaacgttta
    54661 tatttttgta ttttttgcaa ataaaacttt ataaaaagaa tttttaatat gatattctat
    54721 caagtttaaa aataaaaata cttgaaaat
//
LOCUS       DAELSY010000007        51800 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.007, whole genome shotgun sequence.
ACCESSION   DAELSY010000007 DAELSY010000000
VERSION     DAELSY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.007"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(65..247)
                     /locus_tag="KPG09_000353"
     CDS             complement(65..247)
                     /locus_tag="KPG09_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964982.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMVKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            560..979
                     /locus_tag="KPG09_000354"
     CDS             560..979
                     /locus_tag="KPG09_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HBG0964983.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            1110..2951
                     /locus_tag="KPG09_000355"
     CDS             1110..2951
                     /locus_tag="KPG09_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0964984.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFVENKIGVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKHADLLKDMQVSSLC
                     NIKIGECECSFENYECSIEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNQSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYTIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKN
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIETEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMFMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIREIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            2978..4864
                     /locus_tag="KPG09_000356"
     CDS             2978..4864
                     /locus_tag="KPG09_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0964985.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKEDSDIISKIYIYLT
                     LSAISIRKYDKANNFYFSAKKVIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSKNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELRKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYG
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNGTRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            4872..6872
                     /locus_tag="KPG09_000357"
     CDS             4872..6872
                     /locus_tag="KPG09_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0964986.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTREFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            7134..8393
                     /locus_tag="KPG09_000358"
     CDS             7134..8393
                     /locus_tag="KPG09_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0964987.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVEEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            8454..10241
                     /gene="iorA"
                     /locus_tag="KPG09_000359"
     CDS             8454..10241
                     /gene="iorA"
                     /locus_tag="KPG09_000359"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBG0964988.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEMNDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            10243..10818
                     /locus_tag="KPG09_000360"
     CDS             10243..10818
                     /locus_tag="KPG09_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBG0964989.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            10871..11941
                     /gene="buk"
                     /locus_tag="KPG09_000361"
     CDS             10871..11941
                     /gene="buk"
                     /locus_tag="KPG09_000361"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG0964990.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            12159..12860
                     /locus_tag="KPG09_000362"
     CDS             12159..12860
                     /locus_tag="KPG09_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964991.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKRSN
                     EHKCKSSIVIKNEQN"
     gene            12919..13425
                     /locus_tag="KPG09_000363"
     CDS             12919..13425
                     /locus_tag="KPG09_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBG0964992.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEMYELLYE
                     IDNNKLNI"
     gene            13600..14622
                     /locus_tag="KPG09_000364"
     CDS             13600..14622
                     /locus_tag="KPG09_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG0964993.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(14769..15077)
                     /locus_tag="KPG09_000365"
     CDS             complement(14769..15077)
                     /locus_tag="KPG09_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG0964994.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(15137..15565)
                     /locus_tag="KPG09_000366"
     CDS             complement(15137..15565)
                     /locus_tag="KPG09_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0964995.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(15788..17245)
                     /locus_tag="KPG09_000367"
     CDS             complement(15788..17245)
                     /locus_tag="KPG09_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG0964996.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            17663..18577
                     /gene="nadA"
                     /locus_tag="KPG09_000368"
     CDS             17663..18577
                     /gene="nadA"
                     /locus_tag="KPG09_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBG0964997.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            18590..19891
                     /locus_tag="KPG09_000369"
     CDS             18590..19891
                     /locus_tag="KPG09_000369"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBG0964998.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            19872..20708
                     /gene="nadC"
                     /locus_tag="KPG09_000370"
     CDS             19872..20708
                     /gene="nadC"
                     /locus_tag="KPG09_000370"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBG0964999.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            20964..21296
                     /locus_tag="KPG09_000371"
     CDS             20964..21296
                     /locus_tag="KPG09_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBG0965000.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            21458..21643
                     /locus_tag="KPG09_000372"
     CDS             21458..21643
                     /locus_tag="KPG09_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965001.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            21670..22755
                     /locus_tag="KPG09_000373"
     CDS             21670..22755
                     /locus_tag="KPG09_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG0965002.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLYNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSTIPKMKEVIGNHA"
     gene            22748..23206
                     /locus_tag="KPG09_000374"
     CDS             22748..23206
                     /locus_tag="KPG09_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965003.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            23241..24332
                     /locus_tag="KPG09_000375"
     CDS             23241..24332
                     /locus_tag="KPG09_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0965004.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKAGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            24337..24561
                     /locus_tag="KPG09_000376"
     CDS             24337..24561
                     /locus_tag="KPG09_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965005.1"
                     /translation="MENIKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            24571..25134
                     /locus_tag="KPG09_000377"
     CDS             24571..25134
                     /locus_tag="KPG09_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965006.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            25095..25724
                     /locus_tag="KPG09_000378"
     CDS             25095..25724
                     /locus_tag="KPG09_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0965007.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            25764..25901
                     /locus_tag="KPG09_000379"
     CDS             25764..25901
                     /locus_tag="KPG09_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0965008.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            25901..26653
                     /locus_tag="KPG09_000380"
     CDS             25901..26653
                     /locus_tag="KPG09_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0965009.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            26666..27151
                     /locus_tag="KPG09_000381"
     CDS             26666..27151
                     /locus_tag="KPG09_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965010.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYENIMVVDGNSINFIYPVSALQTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            27158..27307
                     /locus_tag="KPG09_000382"
     CDS             27158..27307
                     /locus_tag="KPG09_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965011.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(27443..28264)
                     /gene="yidA"
                     /locus_tag="KPG09_000383"
     CDS             complement(27443..28264)
                     /gene="yidA"
                     /locus_tag="KPG09_000383"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBG0965012.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMTLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNEAPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            28756..30450
                     /locus_tag="KPG09_000384"
     CDS             28756..30450
                     /locus_tag="KPG09_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBG0965013.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSHFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGNIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKISKKLGFEDFVEL
                     GYVRMMRSDYREDMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      30782..30892
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            30996..31367
                     /locus_tag="KPG09_000385"
     CDS             30996..31367
                     /locus_tag="KPG09_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBG0965014.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQDNKKTPKWTENVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            31463..32410
                     /gene="trxB"
                     /locus_tag="KPG09_000386"
     CDS             31463..32410
                     /gene="trxB"
                     /locus_tag="KPG09_000386"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG0965015.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            32495..32812
                     /locus_tag="KPG09_000387"
     CDS             32495..32812
                     /locus_tag="KPG09_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBG0965016.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            33081..34367
                     /locus_tag="KPG09_000388"
     CDS             33081..34367
                     /locus_tag="KPG09_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG0965017.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            34479..34952
                     /locus_tag="KPG09_000389"
     CDS             34479..34952
                     /locus_tag="KPG09_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:34611..34613,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBG0965018.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            34984..36294
                     /gene="grdB"
                     /locus_tag="KPG09_000390"
     CDS             34984..36294
                     /gene="grdB"
                     /locus_tag="KPG09_000390"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:36031..36033,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG0965019.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            36551..38083
                     /locus_tag="KPG09_000391"
     CDS             36551..38083
                     /locus_tag="KPG09_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBG0965020.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNSDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            38094..39239
                     /locus_tag="KPG09_000392"
     CDS             38094..39239
                     /locus_tag="KPG09_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBG0965021.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            39549..40628
                     /locus_tag="KPG09_000393"
     CDS             39549..40628
                     /locus_tag="KPG09_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0965022.1"
                     /translation="MSRVKNVVGLLGTKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            40741..41340
                     /locus_tag="KPG09_000394"
     CDS             40741..41340
                     /locus_tag="KPG09_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965023.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLPPKGQE
                     SSQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIAALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(41527..42399)
                     /locus_tag="KPG09_000395"
     CDS             complement(41527..42399)
                     /locus_tag="KPG09_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0965024.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            42816..44219
                     /locus_tag="KPG09_000396"
     CDS             42816..44219
                     /locus_tag="KPG09_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965025.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            44246..45790
                     /locus_tag="KPG09_000397"
     CDS             44246..45790
                     /locus_tag="KPG09_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG0965026.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            45821..47212
                     /locus_tag="KPG09_000398"
     CDS             45821..47212
                     /locus_tag="KPG09_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBG0965027.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            47405..48901
                     /gene="abfD"
                     /locus_tag="KPG09_000399"
     CDS             47405..48901
                     /gene="abfD"
                     /locus_tag="KPG09_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBG0965028.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            49036..49311
                     /locus_tag="KPG09_000400"
     CDS             49036..49311
                     /locus_tag="KPG09_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965029.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            49335..50642
                     /locus_tag="KPG09_000401"
     CDS             49335..50642
                     /locus_tag="KPG09_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG0965030.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            50662..51780
                     /locus_tag="KPG09_000402"
     CDS             50662..51780
                     /locus_tag="KPG09_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG0965031.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
BASE COUNT        20261 a         5530 c         9556 g        16453 t
ORIGIN      
        1 caatcttttt ttattaatga ataaataata tagaatacaa tatacaaaaa agtttcttaa
       61 aatgctaaga taatccccat ttacaaagca acatcattat actgtgtaaa tttgaatcaa
      121 caatgttttc accatatttc accataaaat cttctaattg tgtgtcaata aatgaataat
      181 atccacttat actatcgatg tatgcagtca ttaagtgtat acattcatca cttttatctt
      241 tatacatata tatctccccc tatatacaat atataaattt agatactaaa tcgtatcata
      301 ttaaatagtt aagtgtgtaa catctatgca gttatatatt tatatcctta tattaataac
      361 taaagtgagt tttgacaata gattttggga aactggatgt tcaaagtgta aacttggatt
      421 ttaaaatcat taattaagat agtgtatagg aatatatgta tacataactt aattataaag
      481 attataaaca attactttta ttaactttat ttaagtggta gaataacttg gagattaata
      541 attataagga ggatataata tgagtttata caagtttata tatgatgata aggaatattt
      601 attaaaagag gagaactgta gtgctttaat aaatgatgaa gaaaatccag ttcaaggagt
      661 aagtatttca aaaataattg atatacttaa tgaagcagaa gaagttgatt ttgatgtaga
      721 atattatcaa gaagcatgtc cattatgttt agaaggagta aaagaaaaga aaaaattctt
      781 tcctttttta gaatatcact tttatatttt cagtaaagat ggagaatatg taataagcaa
      841 tatatctgtt gattataaag gactatcttt taataaacta tcaagggcaa ataaagttga
      901 taatagttat attgtaagtg tgataatttg tgaaaattgt caggattata tagttcagat
      961 agaaaattgt atagtttagt gtaaagtatg tcttaattta agagaaattt ctaaattgag
     1021 acatcaattt ttttcaaaaa tagtatggaa aattactaaa tttatgagat aatgtaagtc
     1081 ggaggtgagt tacaatatat agtaaatatt tggaagagat agatgataca attttagatt
     1141 caatgtttaa actaaccttg aatgaaagat tattgactaa taacgatttt gttgaaaata
     1201 aaattggtgt agtaaagaat ttaattgatt gctataaaac tgaacaaaat agtttaatgt
     1261 tattaaaatc aaattttttg ctagctttat tatatggtat tcaagggttt agtgaaaaaa
     1321 tgaaagagta tattttgatt tcctgtaaat atattgataa atgcttaccc aaagattata
     1381 aatttttagc acgattttat tcacaaatag ctattttaag tttaaaaaat gaagatgtaa
     1441 ataaggcaaa tttatatatg gataaattta atcttatttg tgatgaaaat aattttctaa
     1501 ttgaagaaat tatttttaaa tcacaactta tttacattaa ggcaagtaag tgtatagaat
     1561 cttctgtaat aattaaagaa atagaaacat tgtatataaa gataaaagaa attaacgatt
     1621 ttaactgtaa aaaaatatat ttttttatta ttggtaaaat atattttttg tttttggatg
     1681 atgctctaat tgctaaagta aattttttaa aagcaagaaa acatgcagat ttactaaagg
     1741 atatgcaagt atcttcacta tgtaatataa agataggtga gtgtgaatgt tcatttgaaa
     1801 attatgaatg ttccatagaa tatttcaatg aagtaataag caataaaaag tatagaaatg
     1861 taaatataat tcaaaaatat agagcatcaa acaacataac taaaatatta ataaagacta
     1921 gaaattatcc tgtagcaata aataatttag caaaatctga agtatatcta gaaaaaatta
     1981 aaaatcagag cttaaaagaa gtagagaagt ttgatttatt tataagttta gctatgtatt
     2041 atgcaaaaag taatgaaaaa tcttttgaac aatcaatatg ctatttagat agagctaaaa
     2101 acatcttaaa tatgataaaa aatatagaag aatatacaat atatgattta gaaataattt
     2161 atcatcaggc aaagatatat tatatctttg gatattatga caatgcctta aacacaaata
     2221 aaaaactcct tgaaaaatct aaaaatgcta aaaattataa ttatgtgaag ttagcataca
     2281 agaatatata tttgtgtttt gaaaagattc aaaattatga aatgagtatg aaatacttta
     2341 aaatgtatta tgagttaaaa atgatgtata taaaagctcg aaatagaaat tatatagatt
     2401 ccttaaatta tagacatgag tatatagaaa cagaaataaa taacatgaga aaagtaaaac
     2461 ttgatttaga taaaaaaaaa tatgttgacc atttgacaag tgcatataat agaacttttt
     2521 tgaacgattt tttagaaaaa caagaagtta gtgaatattg cacagcattt atgatagatg
     2581 tagattactt taaagaatac aatgatactt atggacacta taatggagat attgctctta
     2641 aaaatataac tataataata aaaaaatatc taaaaaaaga catgtttatg ataaggtatg
     2701 gtggagaaga atttctgata ttatctgtat gtaaagatta taaaaaaagc aaaatatttg
     2761 gaagaaaatt atgtaggatt gtaaagaaat ttttaaacaa taatttgaca gttagtattg
     2821 gcattgatac atgtaaaaat agtagtatag atataagaga aataattgaa aatgctgata
     2881 aagctttata taaagcaaag gaaagtggta gaaatagatg tttgcattat catgatttta
     2941 agaattttta actgttgaaa taggattggt gatttacttg aaaaggagtt taatagaaga
     3001 tttaaagaga aatataaaca agatagaata cgatatgttg actaaattct atctaaattt
     3061 gacagattta aactacatct tggattatca caaagacctt ttaaatacaa aactagaaga
     3121 taatagtaga gtctatactt tatatttaat tagccttata aactatataa aaaaagatag
     3181 agaagcatca tacaactatt tgatagagtc attaaagtat attaaaaaat tgtcattaaa
     3241 agaagattca gatataatat ctaaaattta tatttatctt actttatcag ctatttctat
     3301 aagaaaatat gataaggcaa ataattttta tttctcagca aaaaaagtta taagacaaaa
     3361 tcatttaaat gagttgctag tcttgttaaa tatgagttta tgtgtagaaa taagtgcttt
     3421 atatcagacc aaaacggatg taatagcttt agtagaagaa gtgtcttttt ttaaaacttt
     3481 aaaaaataaa gctcttgtaa gtagggcaaa ttatatattt gggagagttt atctatattt
     3541 atttaataac tttataaaaa gtatggagta cctaattgaa tgtctaaaac tggctcaaga
     3601 aaataattta tattctatag aggctttttg taaatgtata ataagtttgt gttatttaga
     3661 gagtggaaat aatgtagaag ctataaaata cataaataat gtattagatg ttgcgcacag
     3721 taagaactca atatcagtaa ctgaaagagt gtctataaag atagacctta tatgggcata
     3781 tttaaaagaa gatatgaata aagaagcaga agttatttta ttaaagacta taaaattaat
     3841 gaaagttgtt gaaggtgtat ataaggatta tttatattca tttatatttt tatattcagc
     3901 tgaaattgag ttaaggaaag ataattgtag ttttgaaaag gtaaatacct atttaatttt
     3961 atcaaaaaat atatataaaa gttttggcga tgtttttata tataattcta aactagatta
     4021 catatataag ttgtatggag atttatatat aaaatttggt aatatagatg aaggtataag
     4081 ttgttatata aaaggtttaa atttagttaa aaagagttca ttgaatctta gaaaaatatc
     4141 tatattttat gggctaatag caaatggata tgagctaaaa aaagatttta aactggcaat
     4201 agaatattat aaaagtatgg attggtattc tgataggtgg gaaaaaaata attcctataa
     4261 aacatctcaa gcaattcaca aacaatatga actcagacaa aaacaagaga gtttaagatt
     4321 actagtggat gataataaga aattagaaaa tgatatttta aaagatggac ttactaaact
     4381 atataataga agatatttgg aacagcagtt ggatttattt aatggaacca gagacaaaaa
     4441 tatggttgca atactagtcg atatagattg ttttaaaagt tataatgata attatggtca
     4501 tctagcagga gataaagtta tagttaaggt aactgatatt ataagaagtg tatttataga
     4561 tattaccgaa catataatca gatatggggg agaagaaatt ttaatactag tagattctat
     4621 agaatcaatc ggtgttgaag agattgataa tatagaatta tatattaaaa gagttttcaa
     4681 acttcttgaa tttgagggta ttgaacatat atattctaaa ataaaaaact atataactat
     4741 aagtgctgga gtaagtataa aaagatgtcg tagtagaaaa gatgttgaaa tattaattga
     4801 agatgcagat aagaacctat ataaatcaaa aaaagcaggt agaaatcaat acattataat
     4861 ttaaaattga ggtgattttg ttgcgaaaaa aaataggaat tatagcctct gatattgaat
     4921 taaaagaaag aatagaagaa ttatatagag aagatgtaga aaatggaact ataataatcg
     4981 acatacttaa cctagactta atggaaaatc aaggtcgtat acttgttgaa aaaggtgccc
     5041 aagctataat tggtagaggt ggaggttata gtttagtaat agatacagta aatgtacctg
     5101 ttataccaat gaatatgaag tcaactgacc ttttaagagc aatagaaata gcaaagaaat
     5161 attctaaaaa agtagtattg attcttggag acaatgaagt ttcttttgac tatgttggct
     5221 ggagaaatgt cataagtact gaaataactg aagaatggtt tgaatctaag tatgagataa
     5281 gaagtaaagt tgtaaaatat atagaccaaa aagatgaagt tgtaatcgta ggaggaggtt
     5341 tggcatgttc ttttgcaagg caatatggaa ttgatagtgt atttgcaact gctagtgatg
     5401 aatctataag agaagctgta gaatattgta aaaaattatt ggatacatta ggtgaagaaa
     5461 agtttaataa tgaagtgtta cgaaatatac ttgatggtat aaaagatgga gtaattgcta
     5521 tagatagtaa tggtagtata attttgtaca atgaaagtgc aaaaaacatg cttaaagtag
     5581 agagaaaatg tgcacttaat aaatacatct tagatgtatt tcctaaaatg gagtggatgt
     5641 tagattgttt acatgaaaaa gaggatgtag aagatagaaa gataagaaat ataaataatc
     5701 taattgttaa tactaggaca acattaataa aagttgataa tagtacttat ggagttttag
     5761 gaataataca agatataacc aagctacaaa acttagaaag aaaaataaga tttgacttga
     5821 atcaaaaagg tctctatgct agatatactt ttgatgattt tttatttaaa gataaattaa
     5881 ctagagaatt tattgaagaa gctaaaaaaa ttggaaaaag tgattataca actttacttt
     5941 atggagaaag tgggtctggt aaagaaatta tagctcatag tatacacaat ataagtaaaa
     6001 gaaaagatag gccttttgtt gctataaatt gtgctactat agcagaaaat ttacttgaaa
     6061 gtgaactttt tggctatgaa gaaggtgcat ttactggtgc tagaaagggt ggaaaaagag
     6121 gactatttga acttgctcat ggagggactc tttttttaga tgaaataaat agtctatcct
     6181 ttaatattca gacaaaacta ttaagagtaa tagaggaacg tcaaataatg agaattggtt
     6241 ctgattatat aatacctctg gatataagga ttatagcagc aacgaacgaa tctttaaccg
     6301 aaaaaatagt tatgggtaca tttagagcag acttatttta tagattgagt agtttagaga
     6361 taaatatacc tcctttaaga gacagaagag aagatataat acctttgttt aacaattttg
     6421 taaatgaagt attaaaggat gatggtctca atggaataaa cagtatagat gaaaattttg
     6481 ttttgactaa agatgaaatg gataagctat ataattatag ctggcctgga aatgtaagag
     6541 agttaaagac aatagctcaa aaatatgttg tgacaggtaa aattaaattg agacaagata
     6601 gagattttaa aactaaaaaa ttacttccta actctgaagt agacaaattt aattctgaaa
     6661 cgacagcaag tgtagaagtc caagatgaat ctataaatat aagtaagata aatgatggta
     6721 agataagtat agatataaaa gaagtaaata agtatgttga agaaaaaata ataagtatgc
     6781 tttttgctca gggactttct aaaaatgaag ttgcacaagt tcttggaata agcaggacat
     6841 ctttatggaa gaaatacaat aaaaatattt aaatataatt acatataaat tattttgaag
     6901 taatgttaat tttttaaaac aagtgttaag aaaaattaac attacttttt tgtaaatttt
     6961 tattttttta gacaggcata tatatttata agaaaaatag ttaaaacact taatttaata
     7021 atgaaaaaaa taataattct aaacaaaatc aattttacat aaagtgggat ttattggcat
     7081 agtaattgct taatatataa tgtaagagta aatacaattt taggggtgat ggtatgagtc
     7141 aaagtatggt tggtaagcat gcaatgtggc caaaagaaaa tgatgttata tttagcatat
     7201 ctggtagagc tcaagcagca gagaaggctt ttggtatgga taatgtaata aatgcaacaa
     7261 taggcgctct aatggatgat tcaggaaaat taataacaat gaaaactgta tatgaagaat
     7321 ataaagcatt agataactgt gagataggtg catatgcagc tcttgaaggt caaccagact
     7381 acttagaagc agttaaaaaa gtatttttca gagattactt accagaagga catataagag
     7441 tattagcttc accaggagga agtggtgcaa taaaattagc tgtatggaac tatactaatg
     7501 aaggagatga ggtattaaca tctgattggt tttggagtcc atatgtaagt atagctgaag
     7561 aggctaatag aaaagtagtt aattaccaac tttttgatga aaatagaagg tttaattttg
     7621 agtcgttcaa agaaaaattt gtaaatatag cagaaaaaca aggtagagtc tttacaatta
     7681 taaacacacc agcgcataat ccaactggat atagtgttgc agatgatgag tgggataaaa
     7741 tattagactt atcaaaagaa gtcgcaaaag ataaagacaa aaagataata tttttcgtag
     7801 attcagcata tattgacttt gcaggtgatg atgatgtatg tagaaagttt tttaagaaat
     7861 tttctaattt gcctgaaaac gttctcgtat tggttggatt cagtatgtca aaaggattta
     7921 cagcttatgg tatgagaatg ggggctataa tttgtatatc ttcaagtgaa gatgtagctg
     7981 aagagttcca ttactcatgt gttcattcat gtagagcaaa ttggtcaaat tgtaatagaa
     8041 gtgccatggc agtattatct aacattgtaa atgaccctaa aaaattcaaa gaatatgagg
     8101 atgaaaaaga aatatacaaa aacatgctta ctagaagagc agatgtattt gtagaagaag
     8161 ctgaaagagt aggattagaa attcttccat atatagcagg tttctttgta agtataccat
     8221 gtgataatcc aaaagaagta tgtgaagaat tgactaaaca taatttattt gcagttccat
     8281 taaaaatggg cttaagattt gcagtttgtg cagtttctga agataaatgt aagaaagcac
     8341 caagtattat aaaagaagca cttgaaagtt tagaagtaaa aataaataat taatttcatt
     8401 gaacaaattg ggtggactat gaatttaata attgtagatg gagggaattt tatatgaaac
     8461 aattgatgac aggtaatgaa gcaattgcac gtggagccta tgaggctggt gttaaatatg
     8521 catctgcata tccagggact ccaagtacag aaatattaga aaatatagca acatataaag
     8581 atgcaatagt tgcagagtgg gctccaaacg aaaaggtagc acttgaggct gctatagggg
     8641 gttcaattgc aggagcaaga acaatggctt ctatgaaaca tgttggtttg aatgttgcag
     8701 ctgatccaat atttacttat gcatatacag gggtaaatgg tggtatggta cttattacag
     8761 cagatgagcc gggaatgcat tcatctcaaa atgagcaaga taataggatg tatgcaaaat
     8821 ttgcaaagat acctttattt gaaccatcaa ctagtcaaga agctaaagat atgataaaag
     8881 aagcttttga agttagtgaa aaatacgata caccagtttt atatagagtt acaacaagac
     8941 tttgtcattc aaaaggatta gtggagtgct atgatagaga agaagttgaa attaaagagt
     9001 atgttaaaaa tgctaagaaa atggtaactg ttccagcaaa tgctcaaata agaaggggcg
     9061 ttgttgaaga aagaatggaa atccttaaaa aattctcaaa tgaaactgat ttaaactact
     9121 atgaaatgaa tgatacaaaa ataggtgtta tagcttctgg tatgtgttgt aattttgcca
     9181 aagaagtttt tggaaaaaat gcatcatata tgaaactagg atttacaaat ccattacctt
     9241 atgaaaaaat aaaagaattt gcagaaaaag ttgataaaat atatgttata gaagaaaatg
     9301 acccatttat agaagaacaa ataaaagcat atggaataga ttgtattgga aaagatgtaa
     9361 taccaccata tggagaaatg acaccagatg ttttaagaaa agctatattt ggaaaaacaa
     9421 atgatactat agaatacaaa tctgaattag taacaccaag accaccaagt ttctgtgcag
     9481 gatgtccaca tagaggattc ttctatgaac ttggaaagag aaaaaattta atagttggtg
     9541 gagatatagg ttgttatact cttggatttg ctcctccata taatggaata gattatgttg
     9601 tttgtatggg ttctgctttt ggaactgcac atggagctca aaaagttctt aatatgaagg
     9661 atgataatga aaaaagattg gtaggtgtac ttggagactc aacattcttc catacaggaa
     9721 taaatggact tttagatgtt gtttacaata gaggtaattc tatttcggtt atattagata
     9781 atagaataac tggaatgaca ggtcaccaag aaaacccagg ttcagggtat actttacaag
     9841 gtgctaaaac taaagaagtt gatatagaag gattagttaa agcttgtgga atagaacatg
     9901 taagagttat aaaccctaac aatttaaaag aagtaaatga agcactagat tgggctttag
     9961 ctatagaaga tgaaccatca gtaataataa caagatggcc atgtgtactt aaaaaattct
    10021 ctaaagaaga tatagaagaa tttaataatc catttaagac taagtgtaaa gttgaccatg
    10081 ataaatgtat aggatgtaaa ttatgtttga aaactggatg tccagctcta tcatttgata
    10141 aagagaataa gttatcaaat atagatagaa atcagtgtgt aggatgtgga gtatgtgcac
    10201 aagtttgtcc aaaacaagca atagttaagg aggaaaaata atatgactaa gagtatactt
    10261 ttggtaggtg taggaggtca aggaactata cttgctagta aattattaac tatgggattg
    10321 atggaagctg gatatgatgt aaagatgagt gaaatacatg gaatgagtca aagaggaggt
    10381 tcagtttcct cacaagttag atatggagat tgtgttcatt ctccagttat agaaattggt
    10441 ggagcagata tattagtatc ttttgaaaaa atggaagctt taagatggtt caattattta
    10501 aaaccagaag gaaaagcttt agtaaataat cataggatag attctatgac tgttttaatc
    10561 ggaggagctg aatatcaaga aagtgatata gatgcagagt taagtagatt aaatgcaaaa
    10621 gttataaatg cagctgataa agctgaagaa ctaggaaatt caaaaataat gaatgttatt
    10681 ttattaggat gtctagttaa atctatgcaa ttagagtcta ttgattggga aaaaattata
    10741 agtgaaaatg ttaaaccaaa atttgttgaa ttaaacataa aagctttcca tgaaggtatg
    10801 gaaatggtta gcaaatagtt tataatagat ataaatagac tatagaaatt aaggtgaaag
    10861 gaagaaaatt atgacttaca gaatattagc cataaatcca ggttctactt ctacaaaaat
    10921 agcagtatat gatggagaag aacaaattct tgtgaagacg atagaccatc cagctgaaga
    10981 gattgcaaaa tataatacta tacaagacca gtttgaaatg cgtaaggaag cagttttgaa
    11041 tattcttaaa gaaaatagta tagacttaaa atctcttagt gcaatagtag gaagaggtgg
    11101 agttttacca ccagtaaaat caggagcata tctagtaaat gaagaaatga ttgatgtact
    11161 aagacataga ccagtacttg aacacgcttc caatttaggt gctgttgtgg cacatgcaat
    11221 atcagaacct cttggaatca actcatatat ttatgattct gttgcagtag atgagcttat
    11281 agatgtagcg agaatatctg gactttgtgg aatggataga tcaagtgcag ggcatgcatt
    11341 aaatactaga gcaatggctt taaaatatgc taaggataaa ggaaaagatt ataagagctt
    11401 aaacttaata gtagctcaca ttggtggagg agtaagtatt tatcttcatg aaaaaggaag
    11461 aatggttgat atgctatctg atgatgaagg accattttct ccagaaaggt caggaagagt
    11521 acctgctaca aaattagtgg ctgcctgtta ttcaggtcaa tattcagaaa gagaaatgac
    11581 taaaaagata agaggtaaag gtggtatagt ttcataccta aatactgtag atgctagaga
    11641 agttgaaaaa atgatagcag aaggaaatga agaagcaaaa attatttatg aagcaatggc
    11701 ttatcagtta gcaaaaggta ttggagagtt agcaactgta gtagatggaa aggtagatgc
    11761 tataattata acaggtggaa ttgcatattc tgaaatgttt acttcaatgg ttaaaaagaa
    11821 agttgagttt atagcaccag tagaaattat ggcaggagaa aatgagttgg aatcacttgc
    11881 ttttggaact ttaagagtac taaatggaga agaagaagct agaatttata gtgaaaatta
    11941 ataagaaaaa ttaaaaatgt ttatatacta aaaaagtggt atatccgttt tttatacacg
    12001 aaagcttaga gtataggtga aaatgtataa aatttggatt gccacttttt ataattaaaa
    12061 ttttatctta atctaataaa taattctgta aaatttcgta tttatatata tattttattg
    12121 gtatttattg ttattattat tatgaggagt gatttttgat gggaaaaata agtttaaaaa
    12181 gtatgataag attattttca ggttttttta tatttgcaat ttcttctgtg cttatgatta
    12241 atgcacatgt aggtcttatg ccttgggatg tacttcacca aggtttatct ataaagctag
    12301 gcataactat aggtcaggct tctataatgg taggagttgt aattgttata ttggatgcag
    12361 tttttggtga aaatatagga tggggtactt tactcaacat gacatttata ggaattttta
    12421 ttgatttagt aatattttct ggagtgatac ctcatgcaag caacacttat ataggtgtgt
    12481 ttatggttgt tataggaatt attttggcag ctatagcaag ttttctatac ttaggtgttt
    12541 gtttaggaag tggaccgaga gatgggctta tgattgctct tcagaaaaaa actaataaat
    12601 ctgtacgtct tgttagaaca attcttgaaa tactagcatt agttgttggg tggttattag
    12661 gaggttctgt aggtattggt acgcttgtaa gtgctttagg tttaggatat gtattacaaa
    12721 tagtgtttag aatatttaaa tttgacacaa agttgctgaa acatagattt attatagatg
    12781 atataagaga atggaaggaa aagagaagca atgaacataa atgtaaatca agtattgtaa
    12841 taaaaaatga gcagaattaa ttatgctaaa atatatttgt agtttatcta atttatagta
    12901 aagtcaggag attttgaaat ggaattatgg gatttatata atgctgatgg aattaaaaca
    12961 ggaaatgtta taaaaagagg taattctatt gaagaaggtt attaccattt ggcagttgaa
    13021 gtgtggattt taaatagtaa ctctcaaatt ttaattcaga aaaggtctaa aagtaaaaag
    13081 acattgccaa atatgtgggg aatgacaact ggatgtattg tttctgggga agatagttta
    13141 gaaggagcaa ttcgcgaagc aaaggaagaa atagggatag atataactaa agatgagatg
    13201 aaagttttta gaagtatgat tcatgaagat actctttggg atgtatactt agttaaaaaa
    13261 gaatatgata tatccaaagc tattttacag gaggaagaag taagtgatat aaagtgggta
    13321 agcacagatg aaataagaca attactaaaa gagggtttgt tttttgaata tccagagatg
    13381 tacgaattgc tatatgagat agataataat aaattaaata tatgatgtag agcagatttc
    13441 agtataagta agattattta aaatgtatct tctctaaatt ttaatattgt tttatgaaaa
    13501 ttaataagtt atttttagta tttttctact tgttttttga gattaaaata aaaagggcag
    13561 tcattataaa gtcataaaaa tttggggagg atttaaacta tggaagataa caaaggattg
    13621 gcacgtgata gttcaaaata taaaattgtt tctatatctg tcgtagtaat tattctaatt
    13681 acattttggt atatgttaaa tatggtgctt ttaacattta ttatgacttt tattttctat
    13741 aacttgcttg tagccacaag aaaacgaatt aagaagtttt cttctttaaa tattccagat
    13801 tcattgataa ttatagtgtt atatgcacta tttgctatat tgttagtatt gattagctat
    13861 gcagttgtac caataattat agttcaactt acagagctga gtagggtttt tagtgatttt
    13921 gatgtaaatc aatttgctca atctttagga ccaaaattgt atcctattgt ctcaaagctg
    13981 gattttaata aatatatttc acaagcaggg ctacttatag catcaacagc aacaaaagtt
    14041 ggaagttttg gagtaaatat attattggca tttttactga gtttactcct tttacttgaa
    14101 aaaaatgaaa ttaaaaattt tggagataaa ctttctgata gtaagatttc ttttatttat
    14161 aattcgcttg tattttttgg aaagagcttt gttaaaaatt ttggtgaagt aatgaaagta
    14221 caagttatga ttgcctttat aaattctgtt gtttctatga ttttcttagg tttcatggga
    14281 ttccctcaaa tatgggcttt aggatttatg atttttgtgt tgggtcttat tccagtagca
    14341 ggagttatag tgtctttgat tccattaact gtaatagcat ttaatacagg aggaataaca
    14401 aaggtttttg gagttttact tatgatttgt atagtgcatg cagtagaaac atacatatta
    14461 aaccctaagt tgatgtcgaa tagaactaaa cttccagttt gttttgtttt tattatttta
    14521 cttgtaggag aacattattt aggtgtatgg ggattactaa ttggagttcc aatatttatg
    14581 ttccttatgg atattctggg tgttaaattt acttctaggt aatatattaa aataataatt
    14641 tatttttata tataaattat gaattgtttt aaaaaagaac ctataaatag acatcattaa
    14701 taaagacatc tatttgatag gttcaatttt gctttgtata aatactatag aattaaataa
    14761 aaagaaattt atttcatttc aaaagtttca caacttgcat ttctttcatt tatatgaact
    14821 ttttcagctt tgcaaagttc atttttattg tatttacatt ttacagcttc acaatgaatg
    14881 ttatctgtat caacttggtt agtttcatta gaacgactag ttataccaga tgtatatttg
    14941 tcttcaaaag aagcacaata agtattttct gtagtagttg cttgcattcc atctactttt
    15001 atactaccag cataacaata tccactagta ttataagcac aattagtagc agcacaattt
    15061 aaattattat ttttcataaa taacctccta agttttattt aaacttatta tgtgaagtta
    15121 tctaaatttt atacacttat ttacaattac atttatcaca tttaacaact acatctttgg
    15181 aagtttccct tcctaaattt ttattaacat gatacacatc aacattttca gttgatccac
    15241 atatagaaca tttaaaattg ttttctagta gggttctttc tttaaataac ttccaataca
    15301 tagaattgat gtaatcatta taatttacct tgaggcatgt ctcaagaaag tttatgtata
    15361 gattgtctaa atctcctgta tttaattctg tatattctgg ttcaaaatat tcacaacatc
    15421 ctctaaaatc aggctttgta tctgaaataa cacatctaat atcatatcta tagtctaggc
    15481 taggcccctt aaatgaaaga tgtgtgcaaa aagaacaaga tgtttctggt agattttttt
    15541 caagttcatg taatttatta ttcataagta ttcctccaaa aaatttattt taagtatata
    15601 aatgaataga ttattttaaa acttttattt atttatatta gagatttagg ataaatattc
    15661 caataaggag taaatactta agtatttatt actgtattaa ataaaaaaga ggcaaatcta
    15721 acttgagatt gcctactttt ttatcatttt ttaataaaat ttattctaag tattatttta
    15781 tttagattta gtcagccaat ttagtatccg ctttgctttt taactttcta ccagagcgta
    15841 cagttaaaat agcatataat acagtaaata tggcagctaa aacatatgat atatttatgt
    15901 ttaagttaaa tccaatttga gcattaaata tataactgaa tataacaaaa gaataaaaca
    15961 taccaggaat taagcttata atataattct tttcttttat tataaggtaa actgtaatca
    16021 ttgcaaatgc aaatatggca atagtttgat tactccaaga gaagtatctc cacaatatat
    16081 taaatccaga agcactaagc tttgcaaata taagaatggc tatgacagga ataaatatac
    16141 agattgacaa tataacacga tgtttctttt ccttttggtc ataatgaaag taatctgcaa
    16201 gcattaatct taaagatctt aaagctgtat ctccagaagt tattggtaaa acaattacac
    16261 ctattatagc tatgatacca ccaatagaac caagtaaatc tcttgcaacc aaacctatta
    16321 catcaggaga acctactaat tccttaggta ttcctttatt gtaaataccc atagcagctg
    16381 cagcccaaat cattgcaatt aaaccttcta aaatcatcat attataaaaa gtagtttttc
    16441 cttctttttc atttgaaact gatcttgcta taagagtagc ttgagttgag tggaatccag
    16501 aaacgatacc acaagcaact gtaacaaaga atgtaggaat aagaggaata ccatcaggat
    16561 gaataccttt ccaatttgca agacttaaat tagctaaatc ataaccttgt gtaaagattc
    16621 caattccaac accagcagca gataaaagta gaatagctcc aaatattgga tatacctttc
    16681 ctatgatttt atcaattgga aagagtgttg cacacaagta atatagaaag ataagtccat
    16741 aaactatcca gacaactgga ttatttatag tcgatttcat atttaaaatt tgagtaacaa
    16801 ctaaatcccc aggtgtataa ataaaaactg caccaactaa aagcattaaa agacaaagga
    16861 atatattata gacttgaaat actttattac ctaaaaatct actgataaga gaaggcattt
    16921 gtgctccttt ttctcttata ctaatcattc cactcatata gtcatgcaaa gctcctccaa
    16981 atacacagcc aataggtatt agtatgaatg ctattggacc aaacaatata ccttggatag
    17041 gtcctagaat tggtcctgta ccagctatat taagcaactc aattagacta tttttccatt
    17101 ttttcattgg gacatagtct actccatcac tttgagcaag tgcaggtgtt tttctgtcat
    17161 ctgggccaaa tactttttca caataagcac cgtataaagc acctcctatt agtaatatag
    17221 ccaagccgat taagaatgtt gtcataatat caccccattt ataaaatttt ttggtctttg
    17281 tatacatatt atctaaatgt gtagatttat attgaattta attctggaca acctacaaat
    17341 tctataaagt attacaacta atataatttc agaaataaat acatatacat aaccatgtac
    17401 ttagtataca ggtatttggt aatattttca atgttatagc aataaagaat ataaacaaaa
    17461 ttctgataat attttacaaa aaaattaaaa aatataaaaa ttagtttaaa tagatggaga
    17521 aaaaaggaaa atcaattgag aatataaatg tctatatctt aacaatatta agaggtttaa
    17581 aattcttgaa gaaagaatag tcaagtgata taatgggttt acaggtgtat atacactagt
    17641 acatatagaa agcggtgcaa ttatggataa agatttaaca tatcaaatca aagagctaaa
    17701 gaaagaaaaa aatgcaataa tacttgccca tttttatcaa ccaccagaaa tacaagaatt
    17761 agcagatgca gttggtgact catattattt aagtgagatt gctagagatt gtaaagaaga
    17821 agtggttgta ttttgtggag taaggtttat gggagaaagt gctaagatat tatcacctga
    17881 aaaaactgta ttgatgccag taagtaacgc tgggtgtgct atggcagata tggtagatga
    17941 agaaggcgtg ataaaattaa aacaacagta tccaaatgct ttggttgtat gttatataaa
    18001 ctctacagct aaagttaagg cacattgtga tgtttctgtt acatcttcta gtgcaattaa
    18061 aatactagaa aacatagata ataaagaaat tatattcctt ccagataaaa atttaggagg
    18121 atatattgca gaacaatttc cagataaaaa ctttatattt tgggacggat attgtaaata
    18181 ccataacaat ataagagctg aagaaataat tgaattaaaa gataagtata aaaatgcaga
    18241 agtattagta catccagaat gtaaaaaaga aataagagat ttaggagatt atgtaggtag
    18301 tacatccgga ataataaaat atgctactaa tagtaaaaac aaagacttta taatagcaac
    18361 tgaagaaggt atattacatg aattaaagaa aaataatcct aataaaaact tttatattcc
    18421 tggtggaaaa atactatgta cagatatgaa aaagacaaca ttagaaaatt tatatagtac
    18481 acttaaaaat atggaaaatg aagttattgt agaagatgaa atcatggaaa aggctttaaa
    18541 ttcactgctt aacatgcaca aattagcaga aggatagagg gatagactta tgaatttaga
    18601 acaagatgtt ttaatagttg gaagtggagt ttcaggatta tactgtgcat taaatttaga
    18661 taaaagttta aatgtgctag tagtatcgaa atcaacaatt gaaaacaaca atacatatct
    18721 tgcacaaggt ggaatttcaa ctgctagaaa tattgatgat atagaatcct ttgtagaaga
    18781 tacaatgaag gctgggcagt ataaaaatag agttgaagca gttcaagtac ttgcagatga
    18841 atctatagaa aatgtagggc agatagtaga atatggtatg cctttagaca aagaaaatgg
    18901 agagatagat tacacaagag aaggagcaca tagtgtaaat cgaatagttc atagtaagga
    18961 taatactgga gaagttgttt ttaaaacttt attaaaagaa gctaaaacta gagaaaatat
    19021 aactcttata gaagatgcat atcttttaga tatattaaaa gatggcaata aatgtatagg
    19081 agctagaatt tttaaatcta aaaaagagat tcatgttttt tcaaaaatag tagttttagc
    19141 tacaggtgga attggtgggc tttttaaaaa ttctactaat caaagacatc ttacaggtga
    19201 tggaattgca atagctttaa gaaataatat aaaaatagaa aatttagaat atattcaaat
    19261 acatcctaca gccttttatg aagaaaataa tgaaggtagg agaatgctta tatctgaatc
    19321 attaagagga gagggagcta aacttttaaa taaaaataaa gaaagatttg tggatgaact
    19381 tcttcctaga gatgtagttt caaaagctat ttttgagcaa atggaaaaag ataaattacc
    19441 ttatgtgtat ttagatgcaa cacatttaga tagtgaatat ttaattaata gattttcttt
    19501 tatttataat gaatgtttag ctagaggaac agatataacg aaagaatgta taaaggtatc
    19561 tcctgcacaa cattatttta tgggcgggat acatgtagat ttggattcaa aaacatctat
    19621 ggaaaattta tatgcagttg gtgaaattag ttgtacaggt gttcatggag ccaatagatt
    19681 ggcaagtaat tcattacttg aagggcttgt attttcaaaa agagcagcta aaaatataaa
    19741 ctcagttata gataatgtaa aagttaagtt tatagatgta ccagatatgg atatagatat
    19801 agaacaggta aaaaaagaaa ataaaatatt agtcataaaa gaaattgaaa gaacaagtga
    19861 ggattttggc gatgaattat ttgattattg atagaatgat acaggatgct ttgatagaag
    19921 atgttccaag tgaagatata acaactaatt ctattgtgga tgaaaattct aaatctactg
    19981 tagatttaat ttgcaaacaa gatggtataa tagctggctt aggtgtattt aagagagttt
    20041 ttgagatact tggtgatgta gatgtaaaat tatataaaaa tgatggtgat agagtaaaga
    20101 atcgtgaaaa gatagctgta ttaacaggta gtactagaaa cttacttgta ggagagcgtg
    20161 tagcattgaa ttatttacag agaatgagtg gtatagctac tattacaagt aggtatgttg
    20221 aaaagcttga aggtacaaat acaaaattat tagatagtag aaaaacgata cctaatctta
    20281 gaatattaga taagtattct gttaaagtag gtggaggatg caatcataga tttaatttat
    20341 ctgatggaat attgcttaaa gacaaccata ttggggctgc tggtagtgtt aagaaggcag
    20401 ttgaattagc tagaaaaaat acttcttttg ttagaaagat agaagtggaa gtggaaactt
    20461 tagacatggt aaaagaagca atagaagcta atgcagatat aataatgttg gataatatga
    20521 gtttggaaat ggcaaaacaa gcagtagata ttataaatgg aagagccatt gtagaatttt
    20581 ccggaaatgt aaatttagat actatagaag atataggaaa aattggtgtg gatgttgttt
    20641 ctgtaggagc gcttacacat tctgttaaag ctctcgatat aagtatgaag aatttgagaa
    20701 atatataagt tttatattga aataaactta attaaaatag actccttaaa tttgttttat
    20761 aaatttaagg agtctatttt ttattcatta aaattaataa aaatataaaa aaatcaatca
    20821 ataatttaat aaaaatagat aaatactata tacactttta ataatatagc taatatctat
    20881 tttacagata aataagaata aacttataat ataattgtat ctgtaaaatt ttgtaaaata
    20941 taaaaagtat agggggaata aaaatgaaag tagcttatat ttttagtagt actaatacac
    21001 ataaaattct agacaaaatg ataataccac aacttgaaca aggaactcat ggagtagacg
    21061 ttctagggat gtttttcttt atggataata ccttattttt atctaaaggt aatccagtag
    21121 gcgagagact aagtaaatta catgaaaaaa ctgatatgat aataatggct tgtgaccaat
    21181 gtgctataga aagaggtata gagaataatt tggttgatgg agctacaatt ggatgtttcc
    21241 caaacctata tgcttgttta ggcagtgcag gtgtggacca agttataacg ttataaatta
    21301 gtataggata caatccatag aaaaatttat tttctgtgga ttattttaag taaaagataa
    21361 attgtttaaa gtaggggatg aacaaatgtc taaaatttta gttgaattta taaacacttg
    21421 accatcttgt gatgaacatg gagtgtttat aaaaaaaatg tcagaaaagt atgctgataa
    21481 attggaaata aaattatatc aagcagggaa agatttttcg tatataaaaa aatatgggat
    21541 tataacaaaa ggaactttaa taataaatca gaaaaagaaa tatgatagat taaataaaga
    21601 tacaattgaa agggcaatag tagaagctat aaacaataat taaaagttta aaaattagga
    21661 gtatcaaaaa tgaatatatt agaaattgtt ttaaatggag ttaaagatac tatagtagct
    21721 tctatagtta cactgaatgg agcatcagga tggcttatga taagttttat attggcaggt
    21781 gtacttcaca atgttttaac tccaagaagg tttcaaaaac aacttggaaa taagaaattt
    21841 tcttctttaa ttaaaagtac tatctcagga atgtttttac caatatgtag ttgtggagta
    21901 ataccacttg gaataagtat gtattattca ggagcatatt taggaccagt acttgctttt
    21961 atgacatcaa ctccaataat aaatccaata gctgtagttc tttgtctagg tcttttaggt
    22021 cctaaagtta caatcattta tctaatagct ggttttttag taccattttt agtaggattt
    22081 ttaggaaatt gttttggaaa agaagaatta tcactttata atgattcaga ggaagaagta
    22141 atagaattag aagaagaaaa aagaagtttt atggaaaata tgattgatgg tcttaaatgg
    22201 tcttttggtg aattagccct tactattagt aaatatgtag taatgggtat ggtttttgca
    22261 ggatttataa caactatttt tccaaatagt ataattcaaa aatatttagg aaatccaggt
    22321 ttgctatctc ttggaagtat agcaatactt gcatgtgtta tgtatgtatg tgctgtagga
    22381 catataccgt ttatagcagc ccttgttgca agtggagcat ctcctggtat agcgataaca
    22441 tttctaatag ctggagcagc tacaaatcta ccagaactta taagcatgtt taaacttatt
    22501 ggaaaaagaa ctgttattat atattctgta actttgacac tgtcctcaat aataattgga
    22561 tatataacaa atttactttt agtgaataat aattcagtaa atttagatgc aacaaattct
    22621 tcaatacagt tggcaaataa actaatgtta aatatacctg atagttttga gtatgtatgt
    22681 actattttaa ttattttatt tgcatttaaa tcaacaatac caaagatgaa agaggtcata
    22741 ggaaatcatg cttaaaaaga tagttataat atcctgtata gtagttgtgt taatcatttt
    22801 atcaaaaata gttgatgata acatcaaaga agatgcttct attccaaatg tgaataaaga
    22861 aacattagaa tattttagaa aaaattataa agaagacata ataacttgtg ctgaagaaga
    22921 tttaaataat gatgggaaaa aagatttagt agttatatat aaaaaaagca ataattcaaa
    22981 tgaaatggta gtcgtagttt ctgataaaaa tagccactat ataacaaagc caataccagc
    23041 acctatagag aatcaaacta taacctttaa aaatattgat gataaagcac caatagaagt
    23101 aatagtatca gggtctaaaa atggtaatgt aggatatgct atttacagag tagagggtaa
    23161 aaaatttgtt gatttatttg gcgaagatat ggacaaatgt tgctaaaaat aaaaataata
    23221 cataaaggtg ggaaatttat atgaaattat ctaagaagat tcaagcacta atcttatttg
    23281 gtgtaatggg tacatctata ttaacaggat gtagtagtaa accaaaagaa aaagaagaag
    23341 caaaagctgg aaaagatgat tatacaataa cagtaggtta ttataattgt gaccatatga
    23401 ctgcgggacc agtagcagaa tcggcaggaa tatataaaga tttaggtctt aatgtaaaaa
    23461 ctgttggaaa tggtaaagtt cctgaagcta tggcagcagg aaaaatggat gcaggttaca
    23521 taggaactaa ggggttagtt ggagcaatac ctaagggttc accaataaca atagcagcta
    23581 acaaccatac tggtggttct gaatatttaa tagtttcaaa agatataaaa gaacctaaag
    23641 atttaatagg taaaaaaatt gcaacagata tgagtgattt cttatggaca agtgattatg
    23701 gtccagaaac aggtctacca actgacccat caaaatatga agttgtaaat atggattcag
    23761 acaaagataa atatttagct cttaaaactg ggaaaattca agcttttaca tcttgtgacc
    23821 cttggggttc tgtagctgaa aatgacggtg caggaaaaat aatagcatct actcaatata
    23881 aagaaaaagc taatggcaaa gaatataact gttgttcatt ctcattaaac aaaaacttta
    23941 taaaagaaca tccagattta gcaaaaaaat tggttctagc acatacaaaa gcaatagaat
    24001 atatatatac aaatccagct gaagcagcta aaatatttgc aaaatattat aatgtagaag
    24061 aagaagttgc acttagaact atctataaga agactgttgg agaaggtaga actcttactt
    24121 ggaaagttac aggtgaagaa tataaaaata atttacaaat gtataaagat ttaaaggctt
    24181 tagatgatat acctaaatat gaagatacga ttgatacaag cttattggac tcttgtggag
    24241 ctgatgattt tgacaaattt ataaaagaaa aagttaaccc agagtttcct gaaggtatga
    24301 gttatgaaga ctggaaagca aaggtattat aaataaatgg agaatataaa aaatataata
    24361 atacttttta tcattatttt gggaagtata tttataatat tttttaattt tgaagataaa
    24421 gaagaaataa aaggaaaatt aagaataggg gtatctgatg atacctctgg ttttgtaata
    24481 aattatatgg taaatagaga ctactttgaa aatataaaag taagtgatat aatggatatt
    24541 tttacaatta atgattgctg agcaagtaca atgcagtggg ctctgagttc agaggaaata
    24601 gatatagcaa tcatatgtaa agatgctgca aaacaatatg taaatgtaaa tagtggattt
    24661 gaaattgttg gtactgtagt tcaaaattct gatatatttc tattaagtga taataatcct
    24721 gaaaagatag gagtcattca aaatagagat taccagtcag aattggttaa aaaatactac
    24781 aagaatgtgg aagtagctcc attattaggg actgcactag catatggatt agaaagtaat
    24841 ttagtagatg gtgttgtaat agattgcata aagtcaattg ggttagaggg taaaagaaaa
    24901 tctacaacta aattaggaga ttatgatacc tatgtattag ttgtaagtaa agagtttaaa
    24961 aaaagtaaag catatagtga ttttataaca ctttacaata aaagtgttga tgctttaaag
    25021 aataaagcta catttaagaa agcattaaaa gagtacagga atataagaaa tatagatatg
    25081 gaggaaataa gcaattggaa attaaaattt ctaaagataa aagcgataga ttaactaaga
    25141 aaaaaactga tggtagtatg gatattttat ataaaataat actattagtt attatatttt
    25201 tagtctggca ttttgcagca aaagatatag ggagttcttt gttacttcca atgccagtag
    25261 atgtaataaa aggtttcttt ttctgtgtga cagatgcaga gacagtcact aatcttttta
    25321 taacattgca gagagtactt aaaggtttta tgtatgcatt attgtttggg cttccaatag
    25381 gatttattat gggattttct aaaacttttg aaagggtttt atctccagta gtagattctg
    25441 taaggcaagt tccaataatg gcatgggttc ctcttactat agtatggttt ggtataggag
    25501 atggtccaac aatattttta atagcttttt caggggtttt ccctataatt ttaaatacta
    25561 ttcaaggagt aagagcaata tcaaaggatt attataatgc agctagaagt atgggagcat
    25621 cgccaattgt gatatttaca aatgtaattg ttcccgcatc tcttccagat atactaactg
    25681 gttcaaggat tgctataagt acaggatgga tgtctgttat ctgagccgag tttatagcta
    25741 cgagtgccgg tctcggttac tcaatggtac aagctcaaac attaatgcat acagatgtat
    25801 taattgggct tatgatattt gcagctttaa taggatttat aatagatagg gtattaaagt
    25861 ttataaataa aatcctttgt agatggaggt ttgcagatta atgaagttaa gtgtaagaga
    25921 tataaataag acatttgtaa ataatagagt acatacaaag gttttagaag atataagtat
    25981 ggatatagat gatggacaat ttgtctgtct tttaggacca tcaggttgtg gaaaaacaac
    26041 tttacttact ataataggtg ggtttcaaaa atcagaacgt ggagatgttt ttataaatga
    26101 taaaagagtt aaaaaaccgg gcatagatag agcttttata tttcaaaatt atgctttatt
    26161 tccttggaaa actataagag gtaatgttct atttcctatg aaacagcaga aaataccaaa
    26221 agaaaaaaga gaagaaatgc tagaagaact tttggttatg tcagacttga aaggaaaaga
    26281 aaaactgttt ccgcatcaat tatctggagg aatgaaacaa agagtggcca tgataagagc
    26341 tttagcatgt aatccagagg tattactaat ggatgaacca ttaggagctg tagattttca
    26401 aatgagacaa aatttacagg aagagctaga acgaatatgg ataaagaaga aaataactgc
    26461 attaatggta actcatgatg ttgatgaagc tgtatatatg agtgatagag ttatagtaat
    26521 gtcgagagat aaaggaagaa ttattgatga tataaatata gatattccaa gacctagaat
    26581 tagaggaagc caaaaatatg aagagtacaa aaacaaatta actgatactt tatcaaagtg
    26641 ttatgaagtt taaagaggag tagacatgag tcaaatagaa aaatacaata gtttggataa
    26701 tacacaacag aaaataagaa tctctattat ggatatgata atagataaag gttctagtgt
    26761 tacacttcaa gaagtgactg aatatgtaag taaaaagttg aatatagaaa aagaatatat
    26821 agaaaggaca ctacaatatt ttatttatga aaatataatg gttgttgatg ggaatagtat
    26881 aaattttata tatcctgtat cagcattgca aacaaatcat aaagtaactt tgagagataa
    26941 tagaagtttc agcgcaatgt gtgctataga tgctatagga acctcttgca cattcaacca
    27001 agatataaaa ataaactcta tttgtagtgc tacaggtaag gaaatagaag taattataaa
    27061 aaatgaaaag attgagtatg tgaataatcc aaacttaaga gtgcttcata taaatcttga
    27121 taagcatcta aattgggctg ctagttgctg aaatataatg aatttcttct ggacgaagag
    27181 tgattttgat gcttggacaa acgaagcggg attatctgat gatgaagata tatattgttt
    27241 agatattaat gaggcaatag tagaatcata taaaatattt aaactcaaac aaaaggtact
    27301 atcatagata tacatatatg aaaaactaaa aatgaccttt agataaaaat gataggtaat
    27361 atccatacta tataaattag tatagagtag ggatgtgaaa agtatttaaa tctaaaggta
    27421 tatttttagt ttttttagat gattagatag ccttattaag tatatattta tttataacct
    27481 ttgcaactcc attttcatta ttagtatcag taacataatc tgcaatttcc tttatactat
    27541 tacgtgcatt ccccatagcc acaccaagac ctgcatactt tatcatatgt tggtcatttt
    27601 cttcatcgcc aacagcaata gcattttcta taggtatatt tagattcgta cataatgttt
    27661 taagagcagt tcctttatta gtagttttac ctaaaaattc tagataaaaa ggagcagacc
    27721 ttactatagt atatttattg aataaatttt taggtagttg ctgtatgatt tcttccaatc
    27781 tttctggttc atcaactatc ataattttac agattttaat agattcatca attttatctt
    27841 tttgtatata tttgacttct atattattta atttcgcttc atgaagtgta tattttccag
    27901 gtgcttcatt tggtgtaatt aaatttgtca ttgtatttat gtgtatttta gtcttatatt
    27961 ttttacaaac gttattatat ataaaatcaa aatcatctaa agtcatttct acatcatgta
    28021 tgattttctc acttttaact tcttgaataa ttgctccatt aaagcatata acataatcac
    28081 cttcatctgt taaatttagt tgatttagaa gttcttttac accaggaaga ggtctgcctg
    28141 ttgttataac tacttttgcc ccagcccttt ttgcctcctg aatggactca aagacttcag
    28201 gagtaatcct tttttgtgta tttaatattg ttccatcaat gtctagtgcg attaatttgt
    28261 acatatcaac ctccaataaa ttatatactc aaatttcctg tgaaaacgtt gacacagcta
    28321 gtgttgaaca aagtttcagt gggaaatatg gtttgttttg tatactatta tatcaagaga
    28381 taagtaattt agaaagttta ataatattaa tttttgataa aaatattact tatgtgagat
    28441 gagaatgtaa aaactttgta atcaataatt ccaaaattct taattacaaa taatggtatc
    28501 gtttgcattg ttaatataaa taaatgtata ttatatagtt agaagtttga ataaatagac
    28561 tgatggtaat ttgttatatt agttagtttt gtgttataaa atattgtaat tccattaatc
    28621 atatgttatt attcaacatg attataatat aaagaatatt taatatatat aataatttat
    28681 aaaaaagtat tacatagtac atgtaaatct atatttataa atacaataaa gtaaagttgt
    28741 aaaggagtgc tttaaatgaa gttttcagag tttaagtatg aaagacccaa ttataatagt
    28801 atgaaaaaag agtttttgag ttgtgtagaa gatataaata attctcgtaa ttataaagag
    28861 caacaaaaaa atatacataa gattaattta ttaagaaata aaatagaaac gttgtcaaat
    28921 atagcttcga taagatatag tacagacaca tttaataaat tctacaaaga agaaaaaaat
    28981 tattgggatg aatatatgcc tttgtatgaa gaattaaatt cacattttta taatgctatt
    29041 gtaaattcaa aatttaaata tgatttgata aaagagtttg gagaacaatt tttcacaata
    29101 gtagagtatt ctttaaaaag tttttctaaa gaaataataa gtgaattgca ggaagaaaat
    29161 aaattgtgtt cagagtatac tagactttta gcttcagctg agataatgtt tgatgggaat
    29221 ataagaaatt tatctggtat ggggaaattc atgtattcta agaatagaaa aacaagagaa
    29281 ttagcaaaca aagcatatta taatttcttt gaagaaaatg aaactaaatt tgatgacata
    29341 tttgataaat tggttaaact tagagataag atatctaaaa aactaggttt tgaagatttt
    29401 gtagagcttg ggtacgttag aatgatgaga agtgactaca gagaagatat gataaaaaat
    29461 gttagaaaac aagtattaaa atatgttgtt ccaatggcca atgagcttta tgaaaaacaa
    29521 gctaaaagaa ttggtctaga atatttaagt tacatagatg aaggcgtgga atttttaaca
    29581 ggaaatgcaa gtttaaaggg agattcaaga tacataattc aaaatggtaa aaggatgtat
    29641 tctgaactgt caaaagaaac aaatgaattt tttgatttta tgttagaaaa tgaacttatg
    29701 gatttagaaa cgaaaaaagg aaaaggagct ggagggtatt gtacttatat accagattat
    29761 aagtcaccat tcattttttc taattttaat caaactgctg atgatataga tgtgttaaca
    29821 catgaggcag gacatgcatt tcagttgtat atgtctagat ggattgatat gccagaaatt
    29881 aattttccaa cgcttgatag ttgtgaaatc cattcaatga gtatggaatt tataacatgg
    29941 ccgtggatgg atttattttt taaagaagat acagataagt acaaattcac tcacttatca
    30001 tcttcaatta aatttatacc ttatggagta attgttgatg aatttcaaca ctatatttac
    30061 aaaaatccta atgtagataa gtcaaaacga aaagaaatct ggagattttt agagaaaaaa
    30121 tacttacctc atagaaaata tggtgataat tcttttttag agagaggttg ttggtggttt
    30181 aaacaaggac atatatttaa aaatccgttt tattatatag attatgtatt agctcaaatt
    30241 tgtgcgttac aattttggaa aaaaatgatt caagatagag atgcaggatg gaaagattat
    30301 ataaatatct gtaaagttgg aggaacaaaa tcatttttag atattgtgag tatgggaaat
    30361 ttgtattctc catttgatga tggctgtatt gagtctatta taggtgatgt taaatcttgg
    30421 tttgatgaaa taaatgacag caaattataa aaaattatga gaattttatt gtataaaagt
    30481 gaaaaattaa aaaatatata aaaagtgatt atttgttgtg aaaatagtct tgtttttaac
    30541 taaattgata aaataaacta aaaaagattt gtacttaaaa aaaaaagaaa atacaagaaa
    30601 tatgcaatat caacatttgg aaaaaaaagt aaaaatgtat acaaaataca tataaaaaaa
    30661 ctattgcaaa ttttatagaa tgtttatata cttaagtcaa cagagataga aaaaagctat
    30721 ttatagaaaa caaaggataa attgcttaat tttaaatttt ctgaaaaaaa taataatccg
    30781 gatgaagagt atgggagact gctttataaa aagcgaccga agggacaagt ttaatcttac
    30841 ttacccaaaa gattagatga aattctcagg taaaggtgac tgtacttgga cggggctctg
    30901 aaaatgattt taacagggaa gacaaattgg gagatttgtt tttcctgttt tttatttatc
    30961 aagtaataaa taaaagtttg aaagagagta aaaaaatgat aataattacg aataatccaa
    31021 aagtaaaaga agaagttcaa ggcagagaag ttttgtttaa agacacaact tatattggaa
    31081 tcttagaagc aagtagagat ttgatacatg aaggttatga acttttatct catccactct
    31141 atgggagtgt aaaaccaaat gaaacacctt acaggactgt tatactaaag aaaggaaatc
    31201 gtttggatat aaactcttta accttaattg aagaagcaat catcactgca agtaaatttc
    31261 aggataataa aaagactcca aaatggacag aaaatgtcca agacgatttt agagtaattg
    31321 attatgatat attttataat acaatacaaa gaatgcagta tgaataaaaa aatttgttaa
    31381 aaagttattg aaacagccta aatattaaat ctaatttaat attaaataaa atgagaaatt
    31441 taattgggga ggataagtca aaatggaaaa tgtgtatgac ttagtaataa ttggttcagg
    31501 accagctggg cttgcagcag gtctttatgg agcaagagca aagttaaaaa ctctaatcct
    31561 agaaaaagac aagactggtg gacaaattgt aataacacat gaaattgcaa attatccagg
    31621 gtcagttcca aatgcaacag gaccaagttt aatagctaga atggttgaac agtgtaaaga
    31681 gtttggagca gaaatgctaa gagataacat tgttgatact gagctagatg gagatataaa
    31741 agttttaaaa ggggaaaagg cagaatatag agcaaaagca gtaataatag gaacaggagc
    31801 aacaccaaga aagattggtt gtcctggaga aaaagaactt actggtaaag gggtttcata
    31861 ttgtgcaact tgtgatgcag atttctttga agattttgaa gtatttgttg taggtggagg
    31921 agacagtgca ttagaagaag caatgtattt aactaaattt gctagaaaag taacaattgt
    31981 acatagaaga caaggattta gatgtgctaa gagtgttgaa gaaaaagcaa aagctaatcc
    32041 aaagatagag tttttattag atacagtaat tgaagaaatc aaaggtgatg gaatattaga
    32101 atcagttgta tttaaaaata aagttactgg agaaactcat gaatactttg cagatgaaga
    32161 agatggaaca atgggagtat ttgtgttcgt tggattagat gctcaaactg atttattcaa
    32221 aggtaaagtt gatatggatg aaaaaggtta cataataact gatgaagata tgagaactaa
    32281 cataccaggc gtatttgcag caggagattg tagatctaag actctaagac aggttgtaac
    32341 tgcaactaat gatggcgcaa ttgcatctat agtagctgag aaatacattg atgaaaaatt
    32401 tgggaactag aatctgtaaa ttaagaaata gtgtctgtaa atttaatgaa taatatttaa
    32461 aattaaaata aaaatttcaa gggggaataa gaaaatgtta gatttagata aggctacatt
    32521 tgaagaagaa gttttaaatg cagaaggttt tgtatttgtt gatttttgga gtgaaggttg
    32581 tgagccttgt aaagctttaa tgccagatgt tcataaatta gctgagactt atggggataa
    32641 aataaaattc tgtaagatgg atacaacaaa agctagaaga ttagctataa aacaaaaagt
    32701 attaggtctt ccaactatgg ctatatacaa agatggagaa aaagtagacg aagttactaa
    32761 ggatgatgct acagttccta atatagaaaa catgattaaa aaatatcttt aatatgttga
    32821 taaagtatta acattaaaag aaaaaatcta tttttatctg aagataagaa gaaaaaggtt
    32881 tttatgtcca actttgttaa aaaaataatt ttaaataaaa taattttttt acaataaata
    32941 ttttttagta ataaaataaa gatttaaggc ataaaaacac aataatatat ctttaaaaaa
    33001 ataagataaa ttgtaaaatg atataatttt gagaaaaact caaatgcata tacacaaatt
    33061 aaataacagg aggtgcaagt atgcgtcttg aattagggaa aatctttata aaagatattc
    33121 aatttggtga tgtaacagaa gtaaaagatg gagtattatt catcaataag caagagatgc
    33181 tacaagaaat tggtggagat gagcatatta aatctataga tatagaatta gctcgtccag
    33241 gtgaaagcgt aagaataaca cctgtaaaag atgttattga gccaagagta aaagttgaag
    33301 gtaatggggg aatcttccct ggtatcatgt ctaaagttga tactgtagga gaaggaaaaa
    33361 ctcatgcttt aaaaggtgta gctgttgtaa ctactggaaa aatagtaggt ttccaagaag
    33421 gtataataga catgactgga gaaggagcta agtatactcc attttctaaa ttaaataatg
    33481 tagtagtaat agctgaacct attgatggtt taaaacaata cgctcatgaa aaagctgtta
    33541 gaatgatagg attcaaagca gcaatgtact taggagaagt agcaagaaat cttactccag
    33601 atgaagtttc agtatatgaa actaaacctc ttcttgaatc aataaaagaa tatccagagc
    33661 ttccaaaagt aggatatgtt tacatgcttc agactcaagg tttattacat gatacttatg
    33721 tatatggagt agatgcaaaa caaatagtac caactttatt atatccaaca gaattaatgg
    33781 acggagcaat tgtaagtggt aactgtgttt ctgcatgtga caaaaaccca agttatgttc
    33841 atataaacaa tggggttgtt gaagacttat atgcacgaca tggtaaagat ataaactttg
    33901 taggtgtaat tataactaat gaaaatgttt acctagcaga taaagaaaga tcttcaaact
    33961 ggacttctaa attatgtaaa tatttaggat tagatgcagt tatagtttca caagaaggtt
    34021 ttggaaatcc tgatactgac cttataatga actgtaagaa gatagaaatg caaggtgtta
    34081 aaacagttat agttacagat gaatatgctg gacgtgatgg aggttctcaa tcacttgcag
    34141 atgcagatgt aagagctgat gctgttgtaa caggtggaaa tgccaaccaa gttgtagttc
    34201 ttcctaagtt agataaagtt attggacatt tagaagtagt agatgtaata gctggtggtt
    34261 ctgatggaag tttaagagct gatggaacta ttgaagttga aatccaagct ataacaggtg
    34321 caactaatga aacaggattt ggtcatttaa ctgctaaagg atattaagaa gtattttatt
    34381 aaacgattga gtaatgttaa taaaacatat tatagataat tataataagt gttatagata
    34441 tataaaaata tattagaaat ataataagga ggattattat gagtttactt agtaataaaa
    34501 aggttcttat aataggtgac cgtgatggta taccaggacc tgcgatagaa gaatgtgtaa
    34561 aaacagtaga aggagcagag gttgttttct catctacaga atgctttgtc tgaacagctg
    34621 ctggggctat ggacttagaa aatcaaaaca gagttaaaga tgctgctgat aaattcggag
    34681 ctgaaaatgt tgtgatttta ctaggtgctg ctgaagccga agctgcaggt cttgcagccg
    34741 aaacagtaac tgctggagat ccaactttcg ctggaccact tgctggagtt gccttaggat
    34801 taagtgttta ccacgttgtt gaggaaccaa taaaatcatt atttgatgaa agtgtatatg
    34861 aagaccaaat aagtatgatg gaaatggttt tagaagttga agaaatagaa gaagaaatgt
    34921 ctggtataag agaagaattt tgtaaatttt aatgcctaaa agcaaaaggg gtgaaactaa
    34981 gagatgggaa aactaaaagc agtacattat attaatcagt tctttgctgg tataggtgga
    35041 gaagaaaaag ctgatactaa gccacatgtg gcagaaactt tacctccaat aagtttacaa
    35101 cttgataaat tattaggtga agatattgaa atagttggaa cagtagtttg tggagatagc
    35161 tatttcaatg aaaatattga tagtgcaagt gaagaagttt tatcaatggt aaaaggtttt
    35221 gaaccacaac ttttcatagc tggtcctgca tttaatgctg gtagatatgg ggttgctgct
    35281 ggaacaataa ctaaagttgt aaaagatgct ttgaatatac cagctttaac aggtatgtat
    35341 gttgaaaatc caggagcaga tatgttcaaa aaggatgttt atgtagtaga aacttctgat
    35401 tctgcagcag gtatgagaaa ggcacttcct aagatagcta aacttgcagt aaaacttgct
    35461 aatggagaag aaattggaac tcctaaagat gaaggataca tagctagagg aataagagtt
    35521 aactacttcc atgaagacag aggttctaag agagctgttg acatgttagt taagaaaata
    35581 aaaggtgaac catttgaaac tgaataccca atgccaaact ttgatagagt tgacccaagt
    35641 aaagctgtaa aagatttatc aaaatgtaaa atagctttag ttacttctgg tggtatagtt
    35701 ccaaaaggaa atccagatag aatagagtct tcttcagctt ctaaatatgg aacttattct
    35761 atagcaggag ttatggattt aacagaagag acttatgaaa ctgcacatgg tggatatgac
    35821 ccagtgtatg caaatttaga tgcagataga gtattacctg ttgatgttct tagagattta
    35881 gaaaaagaag gtgtaattgg aaaattacat gagactttct atacaactgt aggtaatggt
    35941 acatctgttg caaactctaa aaaatatgct tctgaaattg gagcagcatt agtggctgat
    36001 ggtgtagatg cagtaatatt aacttctaca tgaggtactt gtactcgttg cggtgcaacg
    36061 atggtaaaag aaatagaaaa aactggactt ccagtagttc atatgtgtac agtagtacct
    36121 atttcattaa cagtaggggc taatagaata gttccaacta tagcaatacc tcatccactt
    36181 ggaaatccag cgcttgaccc tacagaagaa aaagccctaa gaagaggcct tgtagaaaaa
    36241 gcattaaatg ctttaactac agaagtagat ggtcaaacag tatttgaaaa ataaaaataa
    36301 cacacacata tattatatag ataaaaatat aaacacacac taaaaataaa aaataactat
    36361 acacaaaaaa aataatatag atttcatgaa tggataaatt ggagttattc attcatgaaa
    36421 tcttaaaaaa tgccaaaata cataaacaat aatagaatca aatatacaat aaaatacaat
    36481 gattagagct tttaataagt ttaataagat taaaaaataa gtgttcataa aatttatgga
    36541 ggtgcacaaa atgacttatc cagttttaaa aggggcaggc tatgtactta ttcatactcc
    36601 agatatgata gtgcaaaatg gaagtacttg tacagttgaa agagcaacaa attcagattc
    36661 agaattttta aaagaagtaa gtaatcatat aagaagttat gaagatgtag ttaactacat
    36721 gccaaatcaa gtttatatag gaaatagaag accagaagaa ttaagagatt taccaatgcc
    36781 atggtgtgaa caaaagattg agggaactag aaatggtaag tttggagaaa taatgcctca
    36841 agatgagttc atagctttaa tgcaaataag tgatgcattt gatttagtaa aattatcaca
    36901 agaatttatt gatgaagtaa agccaaaaat cgaaaataac taccctgaaa ttgcaccatt
    36961 tgtaggtaaa ttaaagggtg atgatataga agaaggtaaa gaattagtag caacacacat
    37021 agctgaagga ttataccatg atggaaaatt tgttggttat gtaaaaagag cacatgatgt
    37081 agatgttaac ttaaatgccc atacaatgtt tgaaaatcta gtagtaaaag catctggagt
    37141 tttatctgct attcaaatgc taagacatag caaaatagac cctgctgaaa tagattatgt
    37201 tattgagtgt tcagaagaag cttgtggaga tataaaccaa agaggtggag gaaactttgc
    37261 taaatcaata gctgagatag caggacttca gaatgcaact ggttctgaca caagaggatt
    37321 ttgtgcagca ccaactcatg cattaattca agcagcagct ttagtaaaag ctggcataca
    37381 taaaaatgta atggtagtag caggtggagc aagtgctaaa cttggaatga atgctaaaga
    37441 ccatgttaaa aaaggtcttc ctgtacttga agatgttgtt ggaggatttg cagtacttgt
    37501 atctgaaaat gatggtgtaa atccagttat aagaactgat ttaacaggta agcatactgt
    37561 tggaactggt tcttcaccac aagcagttat gacagcactt ataacttcag gtcttgatag
    37621 agctaattta aaaataacag atgttgatgt gtattcagtt gaaatgcaaa acccagatat
    37681 aactaagcca gcaggagcag gagatgttcc agaagcaaac tacaaaatga taggggcact
    37741 tgctgttaaa cgtggagact tagaaaagaa agaattgaaa gattttgttt ctaacaaagg
    37801 attaccagga tgggcaccaa ctcaaggaca tataccatca ggagctcctt atataggatt
    37861 tttaatagat gatttaacta caggaaatag aaatagagct atgatagttg gaaaaggtag
    37921 tttattctta ggaagaatga ctaacttatt tgatggagta tcttttatag ctgaaagaaa
    37981 tacaggtgtt actgaagaga cttcaggaat atctaaggat gaaattaaga agataatagc
    38041 tgaatctatg aaaaaactag cattagatat gttagaagaa taggggtgag gatatgtcaa
    38101 aaaaagtaat cgcagatgta ttcttagaag tagctaatgc aatagaaagc ggcgaatttg
    38161 gcaaaaaagt aaaaattggt gttactacac ttggaagtga acatggtgta gaaaacatgg
    38221 ttaatggtgc acaattagca aaatctaatt tatttgatat tgtgcttatt ggaccaaaag
    38281 tggaaactga ccttgaagta gttgaagtta atgatgaaaa agaaatgcat gcaaaaatgg
    38341 aagaattatt agattcagga tatattgatg cttgtgttac tatgcattat aattttccaa
    38401 taggtgtatc tacagtaggt agagtaataa ctccagcaaa aggaaaagaa atgatacttg
    38461 caacaacaac aggaacaagt gctactaata gaatagaggc aatggtaaga aatgccattt
    38521 atggtattgc tacagctaaa tcaatgggta acaaatgccc aaaagttgga atacttaatg
    38581 ttgatggagc aagacaagtt gagaagtgct taaaagaatt aaaagataat ggatatgaca
    38641 tggagtttgc ggattcaatt agagcagatg gtggatgtgt aatgagaggt aatgatttac
    38701 ttgtaggtgc acctgatgtt atggttactg atacattatc tggaaatata tttatgaaag
    38761 tattttcttc atatactaca ggtggagatt atgaagctca aggctttgga tacggacctg
    38821 gagttggaga agactatgat agaaaggtac ttatagtatc aagagcatca ggttctccgg
    38881 tagtagcaaa tgctttaaaa tacgcttatg atgttgtaaa gggagatata agtaatgttg
    38941 caagaaatga atttgcaaag gttaaaaaag caaaatttga tgacattata tcctctctta
    39001 ctaaaaaaga agttaaagct gaaaaagtag aagttaaaat gccagataaa gaaattgtta
    39061 ctcgtcaaat agctggtgtt gatataatgg atttagaaga tgcagtatct gagttatgga
    39121 aaaacggaat ctatgctgaa agtggtatgg gttgtacagg tccaatagtt ttagtaaatg
    39181 aagctaaagg agacttagca gttgaaactt tagttaaagc tggatatact gcaaaataag
    39241 tacatttttt atatgtaaat aagaacatat agttataatt ttattgatta taattatatg
    39301 ttctttttat tttatatctt gtcaatattt atatattaac acaatccatt tatttgattc
    39361 tctagctaat ttattatatt tattcaatta aaattaagct ttctaactaa ataaaaacta
    39421 atgaagtatt aaaatgaaat taataaaata tgaaaaaatt ttaggaataa agtatctgtt
    39481 agataaaaat aagattaatt agtgatataa tataattaag ataaaattta ttatttggag
    39541 gtaaaatcat gagcagagta aaaaatgtag taggattgtt gggaactaaa ggtgttgatg
    39601 cactatatct aacaaagaaa actaatgtaa actatataag tggttttcct gatgaagaag
    39661 catatgcagt gatttgcaaa gatggaaact ttttagttac tgatagtaga tatatggagc
    39721 ttgcagaaaa ggtttgcaaa gattttgaaa ttataaattg gcataatttt gatagaagtg
    39781 tggcaaaggc agttaaaagt gtatgtgata aagtgggtat aaagaaactt gggtttgaaa
    39841 gaactaatat agtgtttgat aaatatgaag aacttaaaaa tctgattgag aaggataatg
    39901 gagaattaat acctacagaa aatattgtag agactttgag atatgtaaaa gacaaagatg
    39961 aaatcaaaaa tacaagaaaa gcttgtgaaa tagcggataa ggcattagaa gaattgattc
    40021 cacatataaa agctggagtt agcgaaattg aactggctac aaagttagag tactttatga
    40081 aaatgaacgg agcacaaaat atagggtttg aaactatact gatatcagga gcaaagactt
    40141 cattgcttca tggtaagcct agtgataaga ttatagaaaa aggtgatttt gtacttatag
    40201 actatggtgc tatgtataat ggctatatat ctgatacaac tagaactttt atagttggag
    40261 gagcttctga aaagcaatta gaaatatata atttagttaa ggaagctcaa aatgtagggg
    40321 ttgagaacat gaaagctggt gtacatgcga caattccaga tgctgagata agaaaagttg
    40381 tcaaaaaata tgaagactat tactatcaag gtataggaca tggagtcggt agggatgttc
    40441 atgaagaacc tttcattggt aattatggtg ataaaatcat agaagaggga tgtataataa
    40501 caatggaacc aggtatttat ttccctggat ggggtggagt tagaatagaa gatactgttt
    40561 tgattactaa aaatggtcca gaaagactta ctaaattccc taaagatttg atgattttag
    40621 acaaataatt taatttatat atattttttt acataaaata taacataatc tcactattaa
    40681 gctctattta catattatag taaagagggc ttaattttat tgtaagaggt gatagtatat
    40741 atgtataaaa aacataaatt tataaaaaat ataaaaaaag cctggtataa aatcaaatta
    40801 attgatagat gccttataat tgtaatggca atattaatgt ttcaatctat atataattta
    40861 tttgttaatg aagtaaattc acaagatact acaactattg atgtagtagt aagaaccact
    40921 tcagcagcta tatttgggta ttttttaagt gctaatttta taaagagacc tttaaggaag
    40981 tatacaaata ctgatgtatc ttcaaatctt tttatagata ataatttacc cccaaaaggt
    41041 caagagtcta gtcaaaataa tataatgaat gtcaaaaata ccattggatt tacttcagaa
    41101 gctgataatt atgaaaagaa aatacctatt aataataatg aaggttttga agaaggagaa
    41161 actagtgaat tgcaaattat tatagctact gtaatttgta tagcagcatt aacagtactt
    41221 tttgttgtaa gaaattttac aactacaaca tcagcttctt taggtactat ttcacagatg
    41281 agagattttg tctcaggatg cgtgggtttt ttgctaggtt gtccaagtaa acctgattaa
    41341 aaaagtatta tcaataaaag gtattttaaa aagtatcagt ataataatat ttagatttaa
    41401 atgtatacaa ttataattct ataacattct atattgtatt tttatactga tactctaaac
    41461 tatactattt attaaaatat tttaacattg tatttaaaca aaatattata tctgtataat
    41521 ataaatttag tttatatgaa tagatgccat atactcaata aaaagtttca tactatgaga
    41581 tatgtattga tttcttctcc aaagaactcc aatatttgca gtaattgcac gttctaatgg
    41641 tattgaacag agattttctg tattttgaaa tatttcttta tacaatattg ttgaagccaa
    41701 gtcattttct agtaaataac gtatggtaga aagttgattt gagtgtaaaa ttatatttgg
    41761 cttagctttt tcaagtcttt ttgtaataat cttatagtga taagaacctt cagataagat
    41821 tacaaaaggt acatttttta acatataatt tgatataatg ggatgttttg ccaattcatt
    41881 tttgggattt gtaactaagt acaattctgt tttatagatt gtctttgaat ctatatcatc
    41941 ataaatatca ctgtcaatta taccaataca aaaatcaagt gtagattctt caatcatttt
    42001 agctccaaca aatgttggaa cttcaaaaat tttaagctct atatctggat aaattacatt
    42061 aaatctagga agtatttttg aaagcaaaaa cgtacctaaa actgtaggaa tacctatttt
    42121 gagagaagct tttctctttt ttcctaaatc aagtgcgtca cattgaaaat cctcaaattg
    42181 ttttataaaa tgttttgact tgtctaaaaa aatttttcct tcaggcgtta attctatttt
    42241 attacctatt ctgtaaaaaa gttcaaagcc aagttctttt tctaattctt taattgctaa
    42301 tgttatggat ggttgagaaa tatgaatact ttccgcagcc ttagtcatgt ttttatattt
    42361 acagacagtt tgaaaatagt ataaatagtt tatattcatt tggtattagt tcctttcatt
    42421 tacatattag tgacaagtat ataattaaca atagcataag ttttgtatat tgttgtaaaa
    42481 aaatagaata gttttaaact atcatagcat aagtttttga tattttcaat ctattaagaa
    42541 tagtgctatt attaaattaa caaaagagaa tagctggtat ggtttagagt aaatcaatct
    42601 atattacagt tatatcaacg aatatcaaaa aattatctat tagaaaataa aaattatttt
    42661 tacaattaaa ttgttaatta aatcataaag aaaaaagtga tattaacaag ataaaatttt
    42721 tttaatcaga aatgttaatt taataacaaa atagagtatt ggggagaaag gagaaatata
    42781 ttttttaata atatttgtta atttaataac gaaaaatgaa agggagtgat ttagtgaagt
    42841 ttgaattgat ggatttttta atgaatcctt ttgtccttat gtttgctgca gtaattacag
    42901 gaattttatt tggaaaaatt aaatttggga agtttaattt tggggtatca ggagcattat
    42961 ttacaggatt atttattgga tggcttgcat atagcttggg gaatctaata atagcaaaag
    43021 gagaaacagc agcaggatat aaggcagcaa ctgtaatgat ggggaatgga attatttctt
    43081 ctgacttctt tgatttcttt ttaataatat ttgttgcagc agttggtcta ttggcagcaa
    43141 aagatatgaa agctgtatta aagaagtatg gagcaagatt tgtaatttta ggagttctta
    43201 taacttttat aggtggctct atgacttatg caatgacttt actatcaagt gataaaggaa
    43261 gtagtgctta tgaagtttct ggcgtttaca caggagcatt aacaagttcg cctggattag
    43321 ctgctgcttt agaaacagct ggtaaacatg cagaagatgt atcaaaagaa tttgaaaaag
    43381 cttcaataaa agataaaaaa gaaattttaa aagtagtcga cccagaaggc aaattggatg
    43441 tgaatacaac tacatcactg actcaagagc aaatagataa gtatattgca tatgctgaag
    43501 ctggtgttgg tataggtcat gcagtcgcat atccttttgg agttttaata gtaatcttag
    43561 gtgtaaattt ccttccaaaa ttatttagga tggacctaaa agaagaaaga agaaaatatg
    43621 agaaggaaat gaaggaagca agagatagtg tttctggtaa aacgatacca gaagttccat
    43681 ttaatataat aacatttttc ttgacatgtt tagctggata cttagttgga ggaatacatg
    43741 tatttatggg accactaggg tacttcacac taggcgcaac aggaggttca ctaattgtat
    43801 ctctagttct tggatatata ggtaagatag gggtagtaaa tttcagaatg gaagaaaagg
    43861 ttttaaatat tttaaaacaa atagggctag tatttttctt agcaatagtt ggattaagat
    43921 atggtggaaa agtagttgat tcaattatga catctggtat gcacctagcc ttagtagcaa
    43981 tagcagtagg tgttacagct atgatgatag gattcttagt tggcaaatac gtatttaaat
    44041 taaattggat tttattatct ggagctgtct gtggtggtat gacatcaaca ccaggtcttg
    44101 gagctgctgt tgatgctctt gatagtgatg acccagcagc aggttatggt gctacgtatc
    44161 catttgcatt attaactaaa gttatattgg ttatagtttt acacaaactt ccaatgtaaa
    44221 taagatttta tatgggggga tatttatgaa taatgaagga gcatttgaag aattaagtgg
    44281 aagattaaga aataaagaat tgtgtgattt aataatgaat gcagatgaag ctgctaaaat
    44341 tatagaggat ggcatggtta ttggagttag tggatttaca ccttcaggct atccaaaagc
    44401 agtaccatta gcagtttcag aaagagctaa aagtggagaa aagataaaat taaccgtata
    44461 ttcaggagca tctttaggcc cagaagttga tggagcatgg tcagaagcag gtataattga
    44521 aagaagactt ccttatcaaa ctaattcaat acttagaaat aatattaata aaggtgttgt
    44581 tgattatata gatatgcacc taagtcattc tactcaattt ttaaattatg gaactatacc
    44641 taaagtagat gtagctatag tagaagcact tgcaataact gaagaaggga atattatacc
    44701 aactagtggg ataggtaatt ctccatcttt tataaaaagt gcagataaag taatagtaga
    44761 gataaatctt gcaaaaccaa tggaaatgga aggtatggca gatatatata ttacggaaaa
    44821 tccaccaaat agaaaaccaa tagaaataaa tcatcctaag gatagaattg gaacaacata
    44881 tataccttgt ggtttggata aaattgcagg agtagtaatt accaatatgc aagataagac
    44941 ccgtccattg ggggttgtag atgaagcttc gaaaaaaata tcaaataata ttattgcttt
    45001 tttaagagaa gaagtaaagg caggcagatt atcaaagaat ttacttcctc ttcaatctgg
    45061 tgtaggaagt gtagcaaatg cagtattata tggattatgt gaatctgaat ttgaaaattt
    45121 aacttgttat acagaggttg tacaggactc tatgttggat ttaataagaa tgggtaaagt
    45181 tacaatggca tctactacat ctgtaagtcc atctccagaa ggattaataa aatttgaaaa
    45241 agatattgat ttttttaagg ataagattat attaagacct caagaaatta gtaacaatcc
    45301 agaaatagcc agaagaattg gtgtaattgc aatgaacact gcaattgaag ttgatattta
    45361 tggaaatgta aattctactc acataatggg gtcaaagatg atgaatggaa ttggaggttc
    45421 tggtgatttt gctagaaatg gagcaataac tatatttagt actgagtcga ttgcaaaaaa
    45481 tggtgatata tcatcaatag tacctatggt atctcacgta gaccacactg aacatgatgt
    45541 aatggtaatt gtaacagaac aaggatatgc tgatttaaga ggtcttgcac ctagagaaag
    45601 agctataaaa ataatagaaa attgtgccca tccagattat aaagaacaac ttagggatta
    45661 tttaaataga gcttgtaaaa gtggagcaaa acaaacacct cacatattgg acgaagcttt
    45721 atcatggcat agtaaattta tgagtacagg tacaatgaaa aaggctgaaa cttttaaaag
    45781 tgcattataa aatatttaat aaattggagg tagattttaa atggaaaaag cagtggaaaa
    45841 ctttgaagat ttaagtaagg aatacattaa tggttatatt gagagagcaa gaaaagctca
    45901 aagagagttt gaatgttata ctcaagaaca agtagataaa atagtaaaaa tagttggaaa
    45961 agtagtgtat tataatgctg aatatttagc taaacttgct gtagaagaga ctggaatggg
    46021 agtatatgaa gataaggtag ctaaaaataa aagcaaagca aaagttatat ataacaattt
    46081 aaaagataaa aaatcagttg gtataattga tatagataga gaaactggaa taacaaaggt
    46141 agctaaacca gttggagtag tagcggcaat aactccttgt acaaatccaa tcgttacacc
    46201 tatgagcaat gcaatgtttg cacttaaggg aagaaatgca attattatca cacctcatca
    46261 taaagcaatt ggatgtagta caaaaactgt agagatgatt aatgaagaat tagaaaaaat
    46321 aggagctcca gaaaatttaa tacaaatact tgaccaacaa tcaagagaaa atacaagaaa
    46381 tttaatttca tcagcagatg tagtaattgc cactggagga atgggaatgg taaaagctgc
    46441 gtactcaagt ggaaaaccag cacttggtgt aggggcagga aatgttcaat gtatcataga
    46501 tagagatgta gacattaaag aagcagtacc taaaatcata gcaggaagaa tttttgataa
    46561 tggtatcatt tgttcaggtg aacagtcagt aatagtagca gaagaaatgt ttgataaaat
    46621 aatggatgaa tttaaaaata acaaaggttt tatagttaga gataaagtac aaaaagaagc
    46681 tttcagaaat gctatgtttg taaataaatc tatgaataaa gatgcagtag gtcaatcagt
    46741 tcatacaatt gcaaaaattg caggtgttga gataccagaa gatacaaaaa taatagtaat
    46801 agaagctgat ggaccaggag aagaggatat tattgcaaaa gaaaaaatgt gtccagttat
    46861 atctgcatac aagtataaaa gttttgaaga aggtgtagct attgcaaaag caaatttaaa
    46921 tgtagaagga aaaggacata gtgtatctat acattcaaat acagttaaaa atatagaata
    46981 tgcaggagaa aatatagaag tatcaagatt tgttattaat caatgttgtg ctactagtgc
    47041 tggaggaagt ttctttaatg gtcttgcacc tactaatact ctaggttgtg gttcttgggg
    47101 aaataacagc atctctgaaa atcttgatta taagcatcta attaatattt ctagaattgc
    47161 ttattatatg ccagaaaatg aagtccctac agatgaagaa ctttggggat aaaacttaga
    47221 caaaaaatat tcattctaaa ttaaagtagg aatatatttt aatttagaat gaatttaaga
    47281 cataaattaa atttaaatta gtttattgga ctaaatttaa aatattaaga gacatataga
    47341 agaaggaagt attataattt aaaataaata aaaaaatcta taaaagagaa ggagtggttt
    47401 tattatggca ttaatgacag gagctcaata tattgaaagt ttgagaaaat taaatactaa
    47461 ggtttatatg tttggtgaag aggttaagaa ttgggttgac catcctatga ttagaccatc
    47521 tataaattgt gtagcagcaa cttatgactt agctcatgac ccagaatatg cagatttaat
    47581 gacagttact tcaaatatca caggtgaaaa aataaataga tttggtcatc ttcatcaaag
    47641 tgttgatgat ttgataaaaa aagtaaaaat gcaaagacta tgtggtcaaa aaacagcttc
    47701 ttgtttccaa agatgtgttg gtatggatgc gtttaatgca gtgtattcaa caacatttga
    47761 gtgtgataaa gcccatggaa ctaattatca tgataatttt gtaaaatact taacttatat
    47821 acaagaaaac gatttagtag ttgatggagc tatgactgac cctaagggag atagaagtct
    47881 atcacctagt gctcaacctg acccagatat gttccttcat atagtagaaa gaagagaaga
    47941 tgggataatt gtaagaggag caaaggcaca tcaaactggt tcaataaatt cacatgagca
    48001 ccttataatg cctactataa gtatgactga agcagataaa gactacgctg tatcatttgc
    48061 agttccttca gatgctgaag gtgtgtttat gatttatgga agacaatctt gtgacaccag
    48121 aaaattagaa gaaggtgcag atgttgattt aggaaataag gagtttggag gtcaagaagc
    48181 tttagtagta tttgataatg tatttgttcc aaatgataga attttcttat gtggagaatg
    48241 ggatttctct ggtatgttag tagagagatt tgcaggatac catagacaaa gttatggtgg
    48301 atgtaaagta ggagtaggtg atgtaataat aggagcagca gctttagcag ctgattataa
    48361 tggagcaaac aaagcttcac atataaaaga taaactaata gaaatgactc accttaatga
    48421 aagcttatat tgctgtggta tagcatgttc atcagaaggt cacaaaacag aagctggaaa
    48481 ctatcaaata gatttacttc ttgcaaatgt gtgtaaacaa aatgtaacta gatttcctta
    48541 tgaaatagta agacttgcag aagatatagc tggagggctt atggttacta tgccttcaga
    48601 aaaagacttt aaatcagacc ttaaagttgg aacaagtgga atgactattg gcgaagtatg
    48661 taacaagtat tttaaagctt cttcagtggc ttcaacagaa gaaagaatga gaatattaag
    48721 attcttagaa aatatatgtc ttggttcatc tgctgttggt tatagaactg agtcaatgca
    48781 tggggcaggt tcaccacaag ctcaaagaat aatgatttca agacaaggaa acataaatca
    48841 aaagaaagaa ttagctaaga aaattgcggg aataaaaaaa gaggaagctt taaatttata
    48901 aaaagttgat atataaaggg aaattatata ttaatgtgta ttaattaatg aaaagatatg
    48961 tagtataaga gatagttata aaattatggt ggtttttagc taagtaaaga aatttttaat
    49021 aaggagaatt tacacatgac ctgtggagtt agattttgtg ggggctgtaa ccctagatac
    49081 gaaagaggga aggctcttac aataatcaaa gagcacttca atggtagggt agattttagc
    49141 atagcacaag agaacgtcga gtatgatttg ctacttataa ttggaggatg cgcaactcta
    49201 tgcgcatcct atcaacaata taaagtgaaa aatgaatgtg ttaagatgtg ggattttagc
    49261 catatagaaa gagttataaa tgtgattgaa tcagcaattt gtgaaactta aaaaagctaa
    49321 atggggggat ttcattgagt tggcaagaat tgtatcaaag taaattatgt agtgctacag
    49381 aagctgtaaa acagattaaa aacggtgata ctgtagtatt tgcacattgt gtaggtgaac
    49441 ctcctgcact agtagaggct atgattgaaa atgctgaaca atataaagat gttgagatta
    49501 aacatatggt aagcttgggt agtggtggat atactgctaa gggaatggaa gctcatttta
    49561 gagtaaatcc aatgtttgta agtggaaatg taagaaaggc aattgaaaat ggagatggtg
    49621 attttacacc tgcattcttc catgaagtac caaagttgct tagagaaaaa agattaaaat
    49681 gtgatgttgt tttagcacag gtaactccac cagatgaaca tgggtattgt agcttaggaa
    49741 catcagttga ttatacttat gaagccatta aaactgctag aacagtaatt gttcaagtga
    49801 atgaccagtt tcctagaacc tatggtgagg tagtccatgt atcagagttt gactatatag
    49861 tggaaaaatc acaaccttta tttgaactac aacctgcaaa gattggagaa gtagaagaag
    49921 caattggtaa aaattgtgct tcattaattg aagatggttc tactttacag ctaggtattg
    49981 gtgggatacc agatgcagtt atgttatttt taactgataa aaaggattta gggattcatt
    50041 cagaaatgat ttcagatggg actttggccc tttatgaaaa aggtgttata aatggtaaat
    50101 ataaaaattt tgataaagaa aaaatgacag ttaccttctt aatgggtact aaaaaattat
    50161 atgactttgc aaataataac cctgcagtag aggtaaaacc agtagactat gtgaatcatc
    50221 cagcaattat aatgaaacaa cataagatgg tttctataaa ttctgctata caagttgatt
    50281 taatgggaca ggtagttgca gaggctatgg gacttagaca atttagtggt gttggaggtc
    50341 aagttgactt tatacgtgga gtgtctatgg gggaagatgg aaaggcaatt atagctatgc
    50401 cttcaataac tacaaaaaaa gatggtacag taatttcaaa aatcgtatct attgtggatg
    50461 aaggtgcacc tataacgaca tcaagaaatg atgttgacta tattgtaaca gaatacggta
    50521 tagcagaatt aaaaggaaaa tctcttagag aaagagcaag aaatttaata aatatagctc
    50581 atccaagtgt aagagaaagt ctagcagtag aatttgaaaa gagatttaag gagaaatatt
    50641 aataatagag gggtgttaat aatggaagca cttagagtag ttccaaagat attttatttt
    50701 gatacattta aagaatttaa tgaagaattt aaaataggaa aaaatgattt agtaattact
    50761 aatgagttta tatatgagcc atatatgaaa cctcttggaa tagatacaaa tttaattttt
    50821 caagaaaagt ttggaacagg tgaaccatct gatgaaatga tagactccat gacaaaggaa
    50881 atgaagaaat acaattttga tagaattatt gcgtttggag gaggaacaat agttgatata
    50941 tgtaagatat tagcacttga tgttccagaa aagtctattg atttatttga aggagaagta
    51001 tcacctaaaa aagtaaaaga gttagtagta gttcctacaa catgtggtac aggtagtgaa
    51061 gtaacaaatg tagctattgc agagttaaaa tctaaacata ctaaaaaggg gcttgcagta
    51121 gaagaaacat atgcagacta tgcagtgtta attccagaga cagtaaaagg gttaccttac
    51181 aaattctttg ttacaagttc ggtagatgct cttatacatg ctattgagtc ttatctttct
    51241 ccaaaagcaa gtccatttac agagatgtat tctctacaag caataaaaat gattatggat
    51301 gggtataaga agatagttga taaaggagaa gaagagagat ttaatcatct tagagacttt
    51361 gtacttgcat caaattatgc aggaattgct tttggaaatg ctggttgtgc agcagttcat
    51421 gcactatctt attcaatagg tggagctttc catgttgcac atggtgaagc aaattatcag
    51481 ttctttactg aagtatttaa gatgtattct agaaaaaaac ctaatggaaa aattataaaa
    51541 tgtacaaaaa ttttagcaga tgcccttgaa tgtgacccta actgtgatgt ttatggagaa
    51601 ttagaaaagt ttttaaataa attaatagct aaaaaagctt tgagagaata tggaatggtg
    51661 gaaagtcaga tagatgaatt tacagatagt acaatagcaa atcaacaacg actacttgct
    51721 aataattatg tagagcttag tagagaggaa ataagagaga tatttgcaaa tctttattaa
    51781 tacaattttt tatttatatg
//
LOCUS       DAELSY010000008        48513 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.008, whole genome shotgun sequence.
ACCESSION   DAELSY010000008 DAELSY010000000
VERSION     DAELSY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.008"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..550)
                     /locus_tag="KPG09_000403"
     CDS             complement(<1..550)
                     /locus_tag="KPG09_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HBG0965032.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFYFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKTLDLINEDNILNKLIDFNKI"
     gene            complement(580..1689)
                     /gene="ugpC"
                     /locus_tag="KPG09_000404"
     CDS             complement(580..1689)
                     /gene="ugpC"
                     /locus_tag="KPG09_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBG0965033.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDKIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            1948..2685
                     /gene="cas6"
                     /locus_tag="KPG09_000405"
     CDS             1948..2685
                     /gene="cas6"
                     /locus_tag="KPG09_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG0965034.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGN
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            2690..4165
                     /locus_tag="KPG09_000406"
     CDS             2690..4165
                     /locus_tag="KPG09_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965035.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     GNTRNLGKKMIDNGNLSNLKELLLDNISVLRQLTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            4185..5198
                     /locus_tag="KPG09_000407"
     CDS             4185..5198
                     /locus_tag="KPG09_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBG0965036.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIIFGKKGNIYPSIKQNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNSYRGSTQLITNRGFLSSEFGRKYYDEKEENEVLR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDKLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            5214..6020
                     /gene="cas5"
                     /locus_tag="KPG09_000408"
     CDS             5214..6020
                     /gene="cas5"
                     /locus_tag="KPG09_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBG0965037.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIKKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVMPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPY
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCNVCIDTKEVFTWL"
     gene            6047..8455
                     /locus_tag="KPG09_000409"
     CDS             6047..8455
                     /locus_tag="KPG09_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG0965038.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLLEEDKLYIYKSIMLHHGNYEDYMRLSTSKVQEEIYDYIE
                     KGILEKEEFNLKDIESYINDILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDDMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEGALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     NYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            8896..9843
                     /gene="prmA"
                     /locus_tag="KPG09_000410"
     CDS             8896..9843
                     /gene="prmA"
                     /locus_tag="KPG09_000410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBG0965039.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQDGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            9862..10620
                     /locus_tag="KPG09_000411"
     CDS             9862..10620
                     /locus_tag="KPG09_000411"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBG0965040.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            10622..11920
                     /gene="mtaB"
                     /locus_tag="KPG09_000412"
     CDS             10622..11920
                     /gene="mtaB"
                     /locus_tag="KPG09_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBG0965041.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            11963..12313
                     /locus_tag="KPG09_000413"
     CDS             11963..12313
                     /locus_tag="KPG09_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBG0965042.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            12476..12655
                     /locus_tag="KPG09_000414"
     CDS             12476..12655
                     /locus_tag="KPG09_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBG0965043.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            12685..13128
                     /locus_tag="KPG09_000415"
     CDS             12685..13128
                     /locus_tag="KPG09_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBG0965044.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            13239..13736
                     /locus_tag="KPG09_000416"
     CDS             13239..13736
                     /locus_tag="KPG09_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBG0965045.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            13929..14492
                     /gene="raiA"
                     /locus_tag="KPG09_000417"
     CDS             13929..14492
                     /gene="raiA"
                     /locus_tag="KPG09_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG0965046.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            14705..14941
                     /locus_tag="KPG09_000418"
     CDS             14705..14941
                     /locus_tag="KPG09_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBG0965047.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            14954..16297
                     /gene="yqfD"
                     /locus_tag="KPG09_000419"
     CDS             14954..16297
                     /gene="yqfD"
                     /locus_tag="KPG09_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBG0965048.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            16236..17351
                     /locus_tag="KPG09_000420"
     CDS             16236..17351
                     /locus_tag="KPG09_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBG0965049.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKVKMQEKTFKRK"
     gene            17408..17869
                     /gene="ybeY"
                     /locus_tag="KPG09_000421"
     CDS             17408..17869
                     /gene="ybeY"
                     /locus_tag="KPG09_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBG0965050.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            17875..18594
                     /locus_tag="KPG09_000422"
     CDS             17875..18594
                     /locus_tag="KPG09_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBG0965051.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            18713..19114
                     /locus_tag="KPG09_000423"
     CDS             18713..19114
                     /locus_tag="KPG09_000423"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBG0965052.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            19178..20071
                     /gene="era"
                     /locus_tag="KPG09_000424"
     CDS             19178..20071
                     /gene="era"
                     /locus_tag="KPG09_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBG0965053.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            20083..21462
                     /gene="mgtE"
                     /locus_tag="KPG09_000425"
     CDS             20083..21462
                     /gene="mgtE"
                     /locus_tag="KPG09_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBG0965054.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            21535..21690
                     /locus_tag="KPG09_000426"
     CDS             21535..21690
                     /locus_tag="KPG09_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBG0965055.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            21656..22429
                     /gene="recO"
                     /locus_tag="KPG09_000427"
     CDS             21656..22429
                     /gene="recO"
                     /locus_tag="KPG09_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBG0965056.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            22422..23021
                     /locus_tag="KPG09_000428"
     CDS             22422..23021
                     /locus_tag="KPG09_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBG0965057.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            23181..24059
                     /gene="glyQ"
                     /locus_tag="KPG09_000429"
     CDS             23181..24059
                     /gene="glyQ"
                     /locus_tag="KPG09_000429"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBG0965058.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            24059..26125
                     /locus_tag="KPG09_000430"
     CDS             24059..26125
                     /locus_tag="KPG09_000430"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBG0965059.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIRLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(26291..27838)
                     /locus_tag="KPG09_000431"
     CDS             complement(26291..27838)
                     /locus_tag="KPG09_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBG0965060.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            28118..28885
                     /locus_tag="KPG09_000432"
     CDS             28118..28885
                     /locus_tag="KPG09_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG0965061.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            29168..29365
                     /locus_tag="KPG09_000433"
     CDS             29168..29365
                     /locus_tag="KPG09_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0965062.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            29394..30470
                     /gene="vorB"
                     /locus_tag="KPG09_000434"
     CDS             29394..30470
                     /gene="vorB"
                     /locus_tag="KPG09_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG0965063.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            30473..31192
                     /locus_tag="KPG09_000435"
     CDS             30473..31192
                     /locus_tag="KPG09_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG0965064.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            31205..31744
                     /locus_tag="KPG09_000436"
     CDS             31205..31744
                     /locus_tag="KPG09_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG0965065.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            31901..32959
                     /gene="buk"
                     /locus_tag="KPG09_000437"
     CDS             31901..32959
                     /gene="buk"
                     /locus_tag="KPG09_000437"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG0965066.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            32977..33870
                     /locus_tag="KPG09_000438"
     CDS             32977..33870
                     /locus_tag="KPG09_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG0965067.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            33900..35108
                     /locus_tag="KPG09_000439"
     CDS             33900..35108
                     /locus_tag="KPG09_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0965068.1"
                     /translation="MKYAKRMDMVKASAIRASQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDIVVTENPSYLGAINAFRPYECDFLGVDTDDEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            35132..36394
                     /locus_tag="KPG09_000440"
     CDS             35132..36394
                     /locus_tag="KPG09_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0965069.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFSTPVLGMRWVMISLGILSIVMCPVLWFVIKDVDIREDKEEEASEKMSAGDFM
                     TVLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFG
                     APFGGYCADKVKSPTKVLLPIYVFGIAVIILFLVLPASTPMTIFIALTFVVGILGYMG
                     KGIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIF
                     QIAILIIGILSCLYILKVKKKRESKLEA"
     gene            36970..38433
                     /locus_tag="KPG09_000441"
     CDS             36970..38433
                     /locus_tag="KPG09_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG0965070.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSMKLRFFQIKDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSILFSVVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVTSSIALGIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIIANINLVPGAIMTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIIITGMYNVTDVYTTLPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKHIIN
                     VCRFMLIICAAMCCFIEPLTSWALGDICIGVLTYINLVGIFTLKKPAIKAYYDYQRQK
                     KMGVKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            38489..39625
                     /locus_tag="KPG09_000442"
     CDS             38489..39625
                     /locus_tag="KPG09_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0965071.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            39861..40976
                     /locus_tag="KPG09_000443"
     CDS             39861..40976
                     /locus_tag="KPG09_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBG0965072.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALTPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKAKKIGEKELMNLLSLKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            41128..41997
                     /locus_tag="KPG09_000444"
     CDS             41128..41997
                     /locus_tag="KPG09_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0965073.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
     gene            42373..42918
                     /locus_tag="KPG09_000445"
     CDS             42373..42918
                     /locus_tag="KPG09_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG0965074.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            42931..43944
                     /locus_tag="KPG09_000446"
     CDS             42931..43944
                     /locus_tag="KPG09_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG0965075.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            44166..46139
                     /locus_tag="KPG09_000447"
     CDS             44166..46139
                     /locus_tag="KPG09_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG0965076.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKENNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            46208..46567
                     /locus_tag="KPG09_000448"
     CDS             46208..46567
                     /locus_tag="KPG09_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965077.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            46602..46958
                     /locus_tag="KPG09_000449"
     CDS             46602..46958
                     /locus_tag="KPG09_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG0965078.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            46995..48287
                     /locus_tag="KPG09_000450"
     CDS             46995..48287
                     /locus_tag="KPG09_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG0965079.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
BASE COUNT        19279 a         4976 c         8892 g        15366 t
ORIGIN      
        1 taatcttatt aaaatctatt aatttattga gtatattatc ctcattaatt aaatctaaag
       61 ttttatatct aataaggtca aacaaattta aaaacttatt ttcagcattg tcttttataa
      121 tatgaggtag taaatcagaa cataactgta atctaaaata aaaatcagct ttactatcta
      181 attcaccaag ataaaaaata taagtacttt gataagtttc agtttcaatt atattttgtg
      241 tagttacatt aatatctaaa ttatatttag aatttactat aaaaaaacca ttgtgttgta
      301 ttatatttat atcttcaaat aaattataaa aaatattttt taggtattca aagtctatat
      361 gagattggta aattaaataa aagttgcctt ttaaattatc aagattattt ttatctagaa
      421 aatcaataac ataaatagca ttattgaaag taactaaatg gctactagca tctaactttt
      481 ggttttcata gggatagata tttatttttt tattaagctg tctttctaaa aaatctttta
      541 actcttccat aaagtctcct taaaaataag attatattct tatcttatag ctaaagtagt
      601 ttctttgtca aatatgtgta ctctactatt gtcaactgaa agtgttaagt catcaccaac
      661 attaattctg tttttactgt caaatctaat tgttatcgta ttttcgccta gttttaagtg
      721 agcataagtt tctgctccca taagttcttt tatttcaatt cttgatttaa aacaagtatc
      781 tggattagat ttaataaaag tatcttctac atgaatatct tctggtctga tacctataac
      841 aacttcatta ttaatataat tgtcttttat taaatcatag tgtttttctt tattcaattt
      901 aagcttaata tgttcatttt gtgcataaat tttatcattt tcttctatta atattacatc
      961 aataaaattc atctgtggtg ctccaataaa tcctgcaaca aatatattag ctggataatc
     1021 gtatacatct tgtggagttg caatctgctg aactactcca tccttcatta ctacgattct
     1081 atcagccata gtcatagctt caacttggtc gtgtgttaca tagataaatg tagttccaag
     1141 ctgttggtgt agcttagtta tctctgttct catagcagtt ctaagttttg catctaagtt
     1201 ggatagaggt tcatccatta agaatacctt aggatttcta actattgcac gtcctaaggc
     1261 aaccctttgt ctttgtcctc cagataatgt tttaggtttt ttatttaaaa gaggtagtaa
     1321 gtctaatatt ttagcagctt ctttaacttt tttgtctatt tcatctttag gcaattttct
     1381 aagtttaagt gcaaatgcca tgttttcgta aacagataag tggggatata aagcatagtt
     1441 ttgaaaaacc atagctatat ctctatcttt tggttctata tcattgacaa gtttatctcc
     1501 aatgtatagt tctccttcag agatttcttc aagaccagca atcattctaa gtgtagtcga
     1561 tttaccacaa ccagatggtc ctactaaaac gataaattct ttatctttaa tgtctatatt
     1621 tatatttttt actgcgttaa atccatttga gtaaagtttt gatatatttt ttaaaattac
     1681 ttctgacata atattctcct tgatatttaa attttaatga tatagtatca aattttttta
     1741 ttatattaaa tgtgtaaaat gactaaatgt atttgtttaa tatgtatatt taatttttag
     1801 caaagaataa aactaacagt aaaatatatt taaaaaatta aaaaacaaaa ttaaatttat
     1861 tatcaaaaaa tcattttatg atatgtttta ttttttatat tacaaataga gaataatgaa
     1921 ttatactaga aagtaggagg tgaaaatatg agcagaattt caatttttat ggagtcatgt
     1981 aatggcaaat ttccaaatga aaattcaatg ttgtcagttt cctttataaa aaatatttta
     2041 aatgcagaaa acaaagattt tgctaaaaat atatttaact atggggaaga aagaaaaaac
     2101 aataagcaaa taaaggatat aaatacagcc atctacatac caaatcttat aagaagcaaa
     2161 aatgagttgc ttgttgatgg agatataata ttcaatataa gtttttataa ttattctatg
     2221 ttcagtaaat tatacaatgg aattttaaaa gtaaaggagc tagaatataa aggttataga
     2281 tttaaaataa aaaatataaa aatacataaa gaacacgaga taaaagaaaa tggtgttata
     2341 tttaaaacta tgtctcctat aatagtaaag aataaagaag gtaaatattt agatgtagaa
     2401 gattcaaatt atatagaatg tttaaactat atagctaatc taactctgga gagtataaga
     2461 ggtagtgggc ttagaaagcc attggaattt atacctctta acttcaaaaa aagagtgtta
     2521 aaagaaaaga ttcgtggatt taaagtgaga gaatattact atataaatgc ttatgcagga
     2581 acattttttt taaaaggaaa tatggaagat ttaaatgcac tgtataaaat gggaattgga
     2641 tatagaagga cggaaaatgc aggaatggtt gatatattaa agtagcgagg tgagagaaat
     2701 aaaagctaaa ttttatagat caggtgatat tgtaagagat attggcattg tttactttta
     2761 tgaattacta atggatttaa aaaacgaact agaaaaaaat gattataagc taaaatttac
     2821 ttgtgagtta aatagaaatt atttatcttt agaatctgca gaatatatag accctaagta
     2881 tatatctgat tatatattag aaaatcaact ttttaaggta tttaaaaatg ggaaaaagga
     2941 aaccttagaa aaaatattta gtaatataga taatttaacg tatgtaaatt ttattgaaga
     3001 attagataaa tcttctgcaa ctgagaaaca aaaggaaggg attaaaaaag attttaaaaa
     3061 cagaagattt ccatatgtta gaaatagtgg taagtatgga ttaaacacga gtttagaaaa
     3121 tatggaaaca aatgtaaaaa gaatagtaga tactgtaatt aaatcaaata tagagataga
     3181 tgaaatagat aaacttgatt tgacacaata tgaagaaaaa gatagtgtat gtaatgtttg
     3241 taatataaat aaaacaacaa aactagatat tgatttaaga gagcgaaagg actctaagta
     3301 taacttttta tttagaggtg cagaaaaatc aggctttaaa agaagtggac aagttgaaag
     3361 taatatttgt tttgagtgtg aatttttcaa cttaatgtgt cttttatata taaatttaaa
     3421 aagaccaatg gtttttgcat atacgaatga tttaagggaa ttagcatttt taaatcataa
     3481 gataatgctt aaaagaaaga tgtattcaga taaatcattt tataaaaaac ttttgcatga
     3541 aaaaataagt tcgattagac tatataggtt tgatattgat acgaataaag gtataattct
     3601 aaagtttgac tctattatag agtataaaga attattaaaa aaaattgaac ttatagatat
     3661 tatagataat tataattttt caagagagcc agggaatact agaaatttgg gtaaaaaaat
     3721 gattgataat gggaatttat caaatttaaa agaattatta cttgataata taagtgtttt
     3781 aaggcaacta actggaactt ctcgagaaat ggattttgta tctagctcat ataatatcgg
     3841 tttatatata aaattgtgtt ggattgtaga tggaggggag gagtataaaa tgaaaaacta
     3901 tatggaaagt aatttaatat attcaagagt aggtaaagac ttatttaata aaatgacaga
     3961 tgaatccaga agaaaaaatt tttctatgaa agtgattcaa ctacttaagt ctaatgatag
     4021 aactcagcta tttcaaacta taatgcatgt gttagtttct aatggaatct taataggaga
     4081 agggtttgta gaaggtctaa tgcagagttc tgaattagaa ttaaatacga atgtaggtct
     4141 atttattcaa gaaatcatga aataaaaaag ggggaaaatt taaaatgaat gaaaaaagag
     4201 taaaaggagt aacattaact atactgacag aaagcccagt tccactttca tatgaccaag
     4261 ggtatggaaa ttatacacca ataaaaaaag aacaatatag agaaaaaata catgcaaaaa
     4321 ctagtatagc cacaataaca tatgatttaa gaagaatgct ccaccaagaa tatggttgga
     4381 atttaagtaa tattattttt gggaaaaaag gaaatatata tccatctatt aagcaaaatg
     4441 taggatgtgt agatgaaaat ggattagaga ctgatgtatt tggttatttg ataccactta
     4501 aagataaagg ttctataagt aaggcttctc cgcttagaat aatacctttt aggtcattaa
     4561 attcatatag aggttcaaca cagttgataa caaatagagg ttttttaagt agcgaatttg
     4621 gtagaaaata ttatgatgag aaggaagaaa atgaagtact tagagatgaa aactttccaa
     4681 catcacaagc attagcaata gaagagactt tatcagatta ctacgtttat acaataacat
     4741 tggagcttga tagaatagga gtagtagaag tagaagatgg aaagttgctt ttaccagaag
     4801 aaagaaaatt tatgagtaag gagttaagag aaaaagcagt aaaagatata ttagatgcaa
     4861 ttactgtgtt tacaagaaac ataaaacatt catcagtact gttaaaacca cttgcagtaa
     4921 tgggtggagc ttttgataaa gttgtaccgt ttttttggga tgatgtagat tataatgctg
     4981 atagtggtga gataaattta gagggagtca tagaaactat agaaagttat agccttaaag
     5041 aaagtaacac aatacttgct atcaatgaca aattaaaaat ttctaataaa aaggaattaa
     5101 ataagtttaa tttaagtaaa taccctgtaa aagagattaa aaatctagca gatagattgg
     5161 aaattggaga agataatatg tggtatttaa aagagtagga ggttttttga attatgaaag
     5221 tattacaatg taaacttaag caaccaactg ctcactatag agacccaaaa gtatttcaaa
     5281 atgaatatat aagtacttta aatttaccat ctaagaccac aataatggga atgataacgt
     5341 atttatgtga tagaagatta aactctgata tagatatagg aataattgga acacatcatc
     5401 atagagaact agaattttca agaggagaga acattgattt ttggaatgaa tatacaaata
     5461 tgagaaaagg caaagataaa gaaaagtttt tgcttcaagg aaactactat gattactata
     5521 aagaacataa agctcaaaat agcatattaa actatgaagt tcttaaagaa gtagaactga
     5581 ctatatttat ttcttgcaag gatgatgaag aacttgaatt tattaaaaaa aagcttgaaa
     5641 gtccatgtaa atatgcaaat ttaggaagaa aagaagattt tgtaatacca agtgaaaaag
     5701 ggtgttttgt gaaagaagtt gagttaaaag aggttatgcc tatgaatact cgtgatgcta
     5761 taaaagaaaa tataaaatta aagaatacat atgtaagagt tgacttaaga gataaagaga
     5821 atgtcgagac tataataaat caaggtgtat tgattgcatt accttataaa tataaagatt
     5881 tagaagcaaa tagagatgat agagtttatg agttttgcca ttatatatat gttgataatg
     5941 atggtatata tcctaaaaat attaaggtaa atgtatgtaa tgtatgtatt gatacaaaag
     6001 aagtttttac atggctttaa attattttga aagataggtg tttatcatga aaaatatcat
     6061 gcaaaaagaa atattagcta agtcatataa tttaagaact aatcaacaag aacaaacatt
     6121 agttgaacat attaaggatt tatttgaagt attagaatct atattagaac taaatctata
     6181 ttcagataaa gatgtagaga tactaaaaat atgttgtgct ttgcacgatt taggaaaaat
     6241 taattcaatt atgcaacaaa aaatggagat taataataaa atatcgtata gttgctcaga
     6301 agaagagagg aaaaagttag agtctgacaa aaaatcttta aaaaagatag caagacacaa
     6361 tatattttct ggagcatttt taaaagatat attagaaaaa atgaacttgt tagaagaaga
     6421 taagttatat atatataaaa gtataatgtt acatcatgga aactatgaag attatatgag
     6481 gttaagtact agtaaagttc aagaagaaat ttatgattat attgaaaaag gcattttaga
     6541 aaaagaagag tttaatctga aagatataga aagttatata aatgacattt taaatgtaga
     6601 ttttaaattt ggtgaagatg tattggatta tgattacata gataaattaa gtgaaagtat
     6661 gtttatagac agtgattata acaatggtca aatagatgat tcagttttaa attatagaaa
     6721 gtttaagtat attttatata aagggatgct gaacttaata gaccatagtg catcaagtag
     6781 acagaaaggt ataaaatttt ataacgattt tactgatgaa gaaattgatg atatgatttt
     6841 aaatgagata tataagagtc agggtaattt agaaaaaaat aatatagagt tcaatactat
     6901 acaaaaaagg ttgagagaac tttctggaag aaatgttctg actgttgcat ttacaggttc
     6961 aggaaaaaca gctgcagatt atagaaagac ttttaaaaga aaattctttt tagtgccaaa
     7021 taaaatatca gcagaaagtt tttatagaaa aaatattttt caaaataaaa atgatttaga
     7081 tacaagaagt agtaatgatt acattggatt acttcatgga gatataaact tatattcaga
     7141 gaatgaagat aatggagaac atgattttgt attgacatta agggatatag atttgagtat
     7201 aaatttttgt aaaccttgtg taattgcaac agttgaccag ttattactta gtatgtttaa
     7261 atttccggga tatgaaaaaa tattcgcagc cgtaaaaaat gcatctataa ctgttgatga
     7321 agtacattta ttggaaccaa agatgttttt gattttgata tattttatgc agtttgcatg
     7381 caagtactta gatgttgatt ttcacttaat gacagctaca cttccaaaat catataaaga
     7441 gcaaatgata aacaagggaa ttatttttca agaagaaagt aatgaaaatg ttactgataa
     7501 aggagaaata gtttttattg agagcaacaa ggaagaagaa atttgtgaag gtaaagatgt
     7561 aaaggtttct tttataaagg aaaaagaaat caagtcaata gttgaaggtg ctttagaaaa
     7621 taaacaaaaa attcttataa taaagaatac tattgattca gtcaatagaa catatgagtt
     7681 tttaaaagaa aatttatctg ataagtatgg tggtgtagat atagatgttt tacatagtag
     7741 atttaagttc aaagataaaa aagagaaata tagtaaaata ttaaatggag agggtgatat
     7801 ttggatatca actcaatcag tagaaatatc cttggatttg gatttcaata taataataag
     7861 tgatttagca actatggata gcttaataca aagaatgggt agatgcaata gaaataataa
     7921 atatgaatat ggaaactttt atattctacc tagtgaagat aaaatttatg ataacaaact
     7981 taagaacaca actaaaaaca tcttaaagga catattaaaa actaactcta tttttacaat
     8041 ggggattaga aaaactattt tggatgatta ctatgataat agtgtagtta aaaactattt
     8101 tgaagagaat tttataagtt gtgataaaga aattaagaat atttatggta taaataaaga
     8161 aatttttgat ggattggatt taatttttaa ttttgaacct tacaaaaata ttgttgatag
     8221 taagaaggaa gcagctaaaa tttttagaga tgttgatgtt agttacaaaa ttattctgga
     8281 agaagatttt tataaggaag atagggagtt acaacaagat tcgattcaag tatcagggtt
     8341 tatatttaat agattatatt attatggatt aataagcaaa gtagaaggat acatggtact
     8401 aaaatctagt agtaagtttg agtataatag tactgtaggc ttaattttga agtgagcata
     8461 tgtttaaata taatatacct ttagacattg aaaatacaaa aagtactact gtaagcccac
     8521 tgtaaaaact attttttaaa aggaaaaata agttgtgaga tagagtgtct acaagtgtat
     8581 atagataata aatttgaggt ttaagattaa ctaagtgggt ttcaggtaca tcgcgaaaat
     8641 aattataagt tggagatttt gtatgtttaa gattaactaa gtggatttca aatattgata
     8701 tagtagatat aatagaagtt ttattattag gtttaaaatt aactaatcgg atttttaaat
     8761 attctgacta ttatataagg atattattag cttgaattta aaaccaataa tataaaacaa
     8821 caaaatatta tatttatgat ataataaaag ttatggaaaa acaggaatat tactatagag
     8881 aagagaggta aacttatgaa taattggata gaagtaacta taaaaacaac aacagaagca
     8941 gttgaaccta taacgaatat actatatgaa caaggagctg gaggagctgt aatagaagac
     9001 ccaaaagact ttttattcca aaagaaaaat gaactagact gggattatgt tgaagaagaa
     9061 gtatttaaaa agaatgaaga agatgatgtt cttataaaaa cttatgtttc tgaagaaaaa
     9121 aatgtgatgg aatttgtcga aataataaag caaaaagttt taggattaaa agattttggt
     9181 atagatatag gtgaaggaag tgtatcattg gaccaagtaa atgaagctga ttgggcaaat
     9241 gcatggaaag catactacaa accaacaaaa gtaggacaaa gggtagtagt aaagcctaca
     9301 tgggaagatt atgctatgca agatggggat ttaatcatag agcttgaccc tggaatggca
     9361 tttggtacag ggacacatga aactactagt atgtgcatta gagaattaga aaagtatgtg
     9421 aataaagatt ctaaagtatt tgatattgga tgtggtagtg gaatccttgc aatagcagca
     9481 gctaaattag gagcaaaaga agttgtagct gtagatttag atgaagtggc tgtaaaagtt
     9541 gcaaaagaaa atgttcttga aaataaagtt gaaaaaagtg tatctgtaat gcatggtaat
     9601 ttaactgatg taataaaaga taaagcggat gtaatagttg caaatataat agctgatata
     9661 ataaagatac ttgcaaaaga tgttcaaaac tttatgaaag aagatgctat atttatatct
     9721 tcaggtataa ttttggataa agtagaagaa gttaaagaaa gcttaattga aaatggattt
     9781 gaaatagttg aagttcaaaa attaggtgaa tggtcagcaa tagtttctaa gcttaaaaaa
     9841 taagggatat ggtgacgtat aatggataga ttttttgttg aaaaaaataa tataaatcta
     9901 caagataaaa cttgcactat agaaggtgaa gatgtaaaac atatttcaaa agttttaaga
     9961 tgcaaactag gtgagaaact tgaaatatgt gacaagaata ataatgagta tatttgtgag
    10021 ataatgaata tagataagag tatagttaat ttagagattt tagaaaaagt tgatataaat
    10081 agagaatcag aacttaaagt aaggttatac cagggacttc caaaagctcc aaaaatggaa
    10141 atgatacttc aaaaattaac tgaagtgggt gtagaagaaa taatattagt tcaaactaag
    10201 agaagtgttg ttaaagttga tgacaaaaaa gaagataaaa aatttgaacg ttgggaaaga
    10261 ataatttatg aagcagcaaa gcaaagcaaa agaggtaaga taccaaaatt aagaggtgtt
    10321 ttaagtttta aagaagcttt agaagatatg aaaaaaaata atgtaaatat ttgcccttat
    10381 gaaaatgaaa gaacagtttc aataaaacat gcactaaaaa aatgtgattc taatatagat
    10441 agtgttggaa tttttatagg tccagaaggt ggattttctg aagaagaaat agagcaaata
    10501 caaaaaaata attgtaatgt tgtatcattg gggcctagaa tattaagaac tgagacagct
    10561 tctgttgtag catcaacaat agctttgtat gaactaagtg atttaggagg agaaaaataa
    10621 attgaaaaaa gtagcttttt atacacttgg gtgtaaagtt aatcaatatg aaacagaagc
    10681 tatgctagaa ctatttgaga aagatggtta tgaacaagtc aatagtgaag agtatgctga
    10741 tgtttatgtt ataaatacat gtacagttac tcatatgagt gatagaaaat ctcgtcaata
    10801 tataagacga gttaaaaaga agaatccaga tgcaataata gccgttgtag gatgttattc
    10861 tcaggtatct ccagaagaaa ttttggatat agaagaagta aatttagtca tgggtacaaa
    10921 tgatagaaga aagattgttg aagaaattaa aaagataaat tcaagtaaga aagttagtac
    10981 agttgatgac ataatgaaag ttaaagcttt tgaagaaata gaaatcagcc aaactaatgg
    11041 aaagactaga gcatttatga aaatacaaga tggatgtgac agattttgta cttattgtat
    11101 cattccttat gcccgaggac gcgttagaag tagagatatt gatagtatag tcgatgaagt
    11161 gaaaaaattg gctaataatg gctataaaga ggttgtatta acaggaatac atgttgcctc
    11221 ttatggaaaa gatttaaagg atagggatat taaacttctt gatgtaataa aacaaataaa
    11281 ccagatagaa aaaatagaaa gaattaggct tagttctgtt gaaccaattt tatttacaga
    11341 tgaatttgta aatgaagttt taaagatgga taaagtttgt cctcattacc atttatcact
    11401 tcaaagtgga tgtgatgaaa ctctaaaaag aatgaataga cgatacacaa ctttagagta
    11461 caaaactatt gtagatagat tgagaagtaa aatgccagat gtggcaataa ctacagatgt
    11521 aatagtaggt tttcctggag agacaaatga agagtttaaa aaaacgtacg agtttttaaa
    11581 agaaatagag ctttcacaaa tgcatatttt taaatattca ccaagaaaag gaactccagc
    11641 tgcaactatg gaaaatcaag ttgaccctca aatgaaacac tttagaagtg aacaactttt
    11701 aaatttaagt aaagtaaatt ttaataaatt tgcaactaaa tttatcggcc gtgagctgga
    11761 tgttttgttt gaacaaaata tagaaggcaa taaatatgag ggattaactt caaattatat
    11821 aagagttgtt gttgaaagtg acaaaaatat acaaggtcag atattaaaag tgaaaataaa
    11881 tgatgtaaaa gatgaatatg tagaaggaat tttgctataa catgtagaaa ttataatatt
    11941 tataagatag gaggtggaaa gaatggattg tattttttgt aaaatagcga acggagaaat
    12001 accttcaact aaagtttatg aagatgatag agtcttagct tttaatgact tgaacccagt
    12061 tgctccatat catatattag ttgtacctaa aaaacattat gatagtttaa ttgatattcc
    12121 tgataaagag atggacattg tgtcacatat tcatgttgtt attaataaaa tagctaaaga
    12181 gaaaggtttt gaccaaactg gattcagagt cataaataat tgtggaagtg atggagggca
    12241 agaagttaaa catttgcact atcatatttt agctggtaaa aagcttccta attacgaagc
    12301 gggacaaaac taaactaaat tattgaaaaa ttgagttcct tcttgataaa aatacttgat
    12361 aaaagtaaaa atttagttta taataaacaa agtgttaggt tgtaatgcat attatgcaaa
    12421 aagaacatag ttacagtccc agcataattg cgctattgga gggagggaaa aagaaatgtc
    12481 agaagtaaga gttagagaga atgaaacatt agacagcgct ttaagaagat tcaagcgtca
    12541 atgtgcaatg tctggcatca tgtcagaagt gagaaaaaga gagcactatg ataagccaag
    12601 cgttaagcgt aagaaaaaag ctgaagcagc aagaagaaaa aacgctaaga aatagtagta
    12661 aataagaata aagaggtgaa ggggatgtcc cttaagcaaa agttacaaga ggatttgaaa
    12721 tcttcgatga aaaacaaaga tacagtgaga aaatctgtag taactttaat aagagcttct
    12781 ataaagcagt atgaggttga taatagggtc gagcttgatg aagatggaat tatcgatgta
    12841 atagcaaaac aattgaagca acgtagagat gcattagttg aatttgaaaa agctggaaga
    12901 gaagacttaa taaaagaaac agaaggtgaa attgaagttt taaaagagta cctacctcaa
    12961 caattaagcg aagaagagtt agaagaaatt gtaaaatcta ctatatccga agtaggggca
    13021 acttctatga aggatatggg aaaaataatg tcagttatcc aaccaaaagt taaaggaaga
    13081 gctgacggaa aacttataaa taagttggta aaacaaaact tacaatagat tgatatagcc
    13141 tagagagaaa tctctaggct ttttatagtt tttgggaata acgagtcata tttgagcata
    13201 tctataatca aagaaaattt tgaaagaagg ggaaaaattt gattatatca gtaaaagaga
    13261 ttaggaattt tttgataaac atatttatac cactaataat aggttactta agtagcatct
    13321 taacaatgat tatcagtgga acagatattt caacctatta tttacaactc acaaagccaa
    13381 gctttgcacc accagctttt atctttccta tagtttggac tatattgtac atattgatgg
    13441 gtatatcttc atacctaata ttaagaaagg gttacaactt acctaaggta agagatgcaa
    13501 ttttttatta tggattacaa cttgtattaa actttatttg gagtatacta ttttttggat
    13561 ttggacttag atttacagct ttgatagata taataataat gattttggtt tctataataa
    13621 tgatttcgaa gttttctaaa atagataaga gatctggata cataaatttg atttatttaa
    13681 tgtggcttgt ttatgcagga tttttgaact attttatatg gtttattaat aaatagaatg
    13741 taaagatttt gtaaattacc ctgtactttt aataatatca taacaaacga aaaataaata
    13801 gactattaaa aataatcatt ttcatataca ataatattaa tttaaataca ataaatttat
    13861 aaaaattgta tttaaagatt aacatattaa agtattagaa ttggaaaggc ttgaagggag
    13921 aggtttttat gcaaataata gtatctggga gacaaatgaa attaactgac ggaataaaag
    13981 gatacgtaga tggaaaatta agtaggttag aaaaatacct tgatcctgaa tcagaagtga
    14041 aagtaacagt aagtgcgaaa aaggatagac aaaaagtgga ggttacaata attcctatta
    14101 acggtcaaat aataagagca gaagatgtag aagatgactt atatgcagca attgatattg
    14161 tttgtgacaa attaagtaga caagttgtca aatataaaac taaagttaaa gacaaagttc
    14221 aaaataataa gagcataaga tttgaaaatc ttgactttat agataatagc agtgaatttg
    14281 atgattatga ttatgatgat gaagaagatg aaaatatagt aatagaaaga agaaagaaat
    14341 ttaatgtaaa acctatgagt tctgaggagg cgatacttca aatggaacta gtaggacaca
    14401 atttctatat gtttagaaac caagataatt ttgaaataaa catagtgtat aagcgtaaag
    14461 ctggtggata tggtttaata gaacaagatt aataaaaatt gtatacgttt agattttata
    14521 taagaaaaaa taaaaaggta atcatgttga ttttttatca atatgattac ctttttattg
    14581 tttaaataat atattagata ttaggtattt attatataaa aattttaaaa ctaatctgtt
    14641 acaacatcaa taaagtgcat aaatacctat ttcaagcata aaattaatta agggggtaat
    14701 gcttatgtta gaaatatcca ctgatttaca agtaaatcaa ccagttatta ctgttacaag
    14761 taatacgttt attagtatag aaaattatct atcaatatta gaatatgaag ttgatttaat
    14821 aagaataaag actaaagtta aaacaattaa aatatctggg gataaacttt cacttaagta
    14881 tattactgat agtgagatag gtataaaagg gattatctac aatgttgagt atgttgatta
    14941 ggaggagata cacttgataa gttttataag gggatactat gttatagttg tggaaggagt
    15001 aggactcgaa caatttttaa atcatcttat aagaaatggg ataagtgttt acaatgtaac
    15061 tagaataaaa aatactaaaa tggagtttca tatagataga caagatataa aagaatttaa
    15121 gaatgtttat agaggaagta agtttgatat aaaggtaaag caaaaaactg gggtcccttt
    15181 tattatcaaa agagtttata agcacaaagg tatgtggatt tgtgctcttg tttctttatt
    15241 tttattgatg tctacatcgc agtttgtgac agatgtatat atacaatctc ctgaaggtat
    15301 aaaaaaagaa gctcttagaa atgagcttta taaggttgga gtaagaccag gagtctataa
    15361 gaagagtatt gatagaaaag aagttagaga ccatatgatg tcaaaattta atgatgtggc
    15421 atatttatct atcaatgtaa aaggaacaaa tatatttgtt actgttacaa aaaaggcaga
    15481 atccctaaaa tctacagacc aatcaaatta ttgtaatgta attgctttaa aaaatggtat
    15541 aatagaaaag gtaatcccta gaagtggtaa atcagttatt aaatctggag acatagtcca
    15601 aaagggagat gtcttattaa atggagctaa taccaagtca ataccagaag tatgggcaag
    15661 tactttttat gagtccacaa aaaaggcaag ttatgtagat actgtaaata aaaaaactgg
    15721 agaaaagaaa aatatatata cactaagttt ttatgataaa gagtttacaa tgagaaaaaa
    15781 tataaaatat aaagattatg ttgttgaaaa taaagaaaaa aaactatcaa taggaaacta
    15841 tacttttcca ataaagataa aaacgagtac tttttatgaa acaaaaaagg ttgaagtaaa
    15901 aaggaataaa gaagagttaa aaaaagaatt atctgaaaaa gctttaaaag agttagaata
    15961 cataatccca gcttcggcta gaataataga tgtaaaacat aactttaagg ttaataaaaa
    16021 tatgttagaa tatttaataa cagttcaaac tagtgaaaat attgctaaaa tttatcctct
    16081 tagtaagtca gaagcagaaa ggtttataaa ggaagaaagt aaaccagatg aaggtgaaga
    16141 ggaagttcct tccaatccag aaaaaagacc attaaatgat ataagaaatg agtttgatga
    16201 ggataataag gataataaag accaaaacaa ttctgatgaa aataatagta atcaaaatag
    16261 taataatagt caaaataata atagcaataa caactaataa aattaaactt aaaatcttag
    16321 gaggactaat tcaattgata atacaaaaga aatttacaat agcagatggt aactttgaaa
    16381 gagagttatt tggcaacttt gatgaaaatg taaaattaat agagaagact ttaaatatag
    16441 atgtaatact tagagaaggc aatataattt taattggaga agaaaaaaat gtagattctg
    16501 cattaaaact tatgaatgaa ttacaccaaa ctgtatcaaa tggtaagcat ttagataagc
    16561 agagtatttc ttattcatta tctcttttgc ttgaaggaag tgaacaaaaa ataaaagaac
    16621 ttgaggggac aatcgtcata actcaaaggg gaaaagctgt tcaacctaaa actttaggtc
    16681 aaaaagaata tataaaactt atagaaaata atgatataac ttttggtgtt ggacctgctg
    16741 gaacaggaaa gacttatctt gcggttgcta tggcagtaaa agcttttaaa agagatgaag
    16801 ttagtagaat tatacttaca agaccagctg tggaagcagg agagagtcta ggttttttac
    16861 caggagattt aaaggacaaa gttgacccat atttaagacc actatatgat gccttatttg
    16921 aaatgttggg tgctgataaa tttaataagt atttagaaag aggtactatt gaggttgcac
    16981 cacttgcatt tatgagaggt agaacacttg ataattcatt tataatattg gatgaagctc
    17041 aaaatactac ttctgaacaa atgaaaatgt ttttaactag acttggattt ggctctaaag
    17101 ctgtagttac aggagatgta actcaaacag atttaccaca aaataagaag agtggtctaa
    17161 tacaagctac agagatcctt aaaggtgttc caggtattgg gtctataatg ctaacagata
    17221 gagatgttgt aagacatgag ttggtacaga gaataataag agcttatgag aagcacgaca
    17281 aaagagaaga atttaaaaaa gaagaacgta aaaaagtgaa aatgcaggaa aagactttca
    17341 aaagaaagta aaagtgtagt aatatagata tatagcatta ttaatgaata aaaagtaggt
    17401 gtagtgaatg gatttgatac tagatgatag acaagataaa ttagaagtaa gtgaggagtt
    17461 aattgaaaag ataaaggata taatcataga atgtttagat tatgaaggat atgatgataa
    17521 ttatgaagtg agtctttctt ttgtagataa taaagagatt catgaattaa atagagaata
    17581 tagaggagtt gatagagtga ctgatgtttt atcatttcct cttttaagtg atgattttga
    17641 ggatgttgag ttagaagaag aatccctagg tgacatagta gtatctcttg aaagagcctt
    17701 agaacagagt atagagtata accatagttt tgaaagagaa gtatgttttt taatatgtca
    17761 tagtatgttt catcttttag gttatgacca cgatacagat gaaaatacaa aagaaatgag
    17821 agaaaaagaa gaacacatat taaataagtt aaacataaca agggagtagt ttttatgaaa
    17881 ccagaaaaaa ctcgtcaagg tataattaaa gcatttaatg cagctataga agggatattg
    17941 tatacattta aatttgaaag aaatatgaaa atacattatt taggttcagt tgctgttctt
    18001 ataataagtt tgttttttaa cttttcaaaa ttagaaatga taatgttact tatgtccata
    18061 tgcttagttg tagttgccga aatgtttaat acagctatag aaaaagctgt tgatttagta
    18121 actgatgaat atcatgttct tgctaagata gcaaaagatg ttgcagctgg aggcgttcta
    18181 gtggcagcac taaattcagt agtagttgga tatattttgt tttatgataa attgacagat
    18241 atatctggaa tattaattta taaaataaga gaatcagaat tacatataac cttgatatgt
    18301 atattattgg ttcttatagc tgttgtagtt gtaaaagcat tgacttctac aggtactcct
    18361 ctaaaaggag gaatgccaag tggtcatgct gccttagcat ttgctatagc aactgcaatt
    18421 acacttatga ctgaaagagt tgttgcctct actcttgcat atataatggc tgtattagtt
    18481 gcccaaagta gaattgaagg aaaaatacat accttttggg agactattgc tggagcttta
    18541 ttaggtgtgt tgatagctat attagtattt cagcttggaa tgttctataa ttaaaataaa
    18601 tgtacataaa atatttaaaa taaaacaaat ctgtgctaat taaattttta tgatataatg
    18661 tagagtatta aataaaaata acttttaatt attgtataca ggaggagttg ttgtggataa
    18721 catagaattg ttaaggttgg cagaagatgc cagacaacat tcttatgctc cttactcagg
    18781 ttttagagta ggtgcagctc ttttaactaa gagtggtaaa gtttatactg gatgtaatat
    18841 agaatgtgca tcattaggtg gaacaaattg tgcagaaaga acagctattt ttaaagcaat
    18901 atcagagggt gataaggata tctataaaat agctatagca agtgataact cagaaaataa
    18961 tgagcaaaca tatccatgtg gaatatgcag gcaggtaatt atagagtttg gttctgatat
    19021 aaaaataata acgggatata ctaaaggaga ggtgtttgag catagtataa aggatttgtt
    19081 gccaaactat ttctctggaa atgatttaaa ataaatttaa aagtataaat aaatttaagt
    19141 aattaaaaca agaagataaa aagttaggag acagttaatg ttcaaatcag gttttgtcag
    19201 tatagtgggt agaccaaatg taggaaaatc tactttaatg aataatgtag ttggagaaaa
    19261 aatagctata atgagtgata agcctcaaac tacaagaaat acaatacaag cagtatatac
    19321 agatgaagaa atgcagatag tattcttaga tacacccggt attcacaaac caaaaaataa
    19381 attaggtgaa tttatggtaa aggcagcaac agaagctttt aaaaatgtag atttaatatt
    19441 atttgtagtt gatgattcta aaaaaatagg tcctggagat agaaagataa tagaggattt
    19501 aagaagtgtt aagacaccaa taattttagt tgtaaacaaa atagaccaac tagaccaaaa
    19561 agatgagcta tttgatataa tcaaaatgta tgatagagaa ggtattttta aagaaatagt
    19621 accaatatca gcattaaaag gaaagaatac ggatacctta ataaaagtta tacaaaatta
    19681 tttagaagaa gggccaaagt attttccaga ttatatgatt acagaccagc cagaaagagt
    19741 attaatagca gaacttataa gagaaaaagt tcttcactat cttaatgatg aaatacctca
    19801 tggtgtagct gttgaaattg aaaaaatgaa agcgagaaat gataaggaaa tagtagatgt
    19861 atctgctgta atatactgtg aaagagattc acataaggga ataataatag gaaaaaatgg
    19921 taggaaacta aaaggaatag gtaagtcagc aagacaagat atagaattgc ttttaggttc
    19981 acaaataaat cttcaattat gggttaaagt caaagaaaat tggagaaatt tacaaaatta
    20041 tataaataat tttggatata atgataaata aaggtgggtt atatggatag gaaactggat
    20101 gcttcccaag atttattata tgaagtaaaa tcattaattg ataataataa agtattggag
    20161 ttaagagaat taatagagga atatcatata attgatatat tcgatataat ggaaaattta
    20221 gaggaagaca tgaaaattca attatttgaa gtacttcctt tagatatggc atcttctata
    20281 ttagaagaag gtagtgtaga gttttttatt agtattttat ctaaactgga tgtagaacac
    20341 tcgaaaaata tacttgaact tatgtctctt ggagatatgg cagacaagtt gagtgaactt
    20401 gaagaagaag aaagagaaca cataataaat cttttgaatc aagagaatgc cgattatgta
    20461 aaagagttac tattctatga tgaagattct gctggtggaa caatgaccac tggatatatt
    20521 tctataaata aagatatgac agctcttgag gctatagacc atatgagaga agaagctgaa
    20581 gaagctgaaa ctatttatta tatatatgtg gttgatgatg aagaaaaact agttggagta
    20641 ttgtcattaa gagaattaat aatagctaga gacgcaaata tagtagaaga tttgatgagt
    20701 gaaaatataa tctctgttta tgtagatgaa gatagagaag aagcagtaag attagtatct
    20761 aaatacaatt taatagcaat tccagtagta gacagacaag aaaaattaaa aggaataata
    20821 acagtagatg atataattga tgtaatggaa gaagaagcaa ctgaagatat gtataaattt
    20881 gcaggaagtt cagaacatga aagagaagtt gctgaaaaag aaaaccctac attgagagag
    20941 caaataatat cagcacttag aggacgatta ccttggttaa ttattacctt ggttggaggg
    21001 ttgttggctt cgttaatact ttctaactta gactatataa tgaatcctgt atatgctcca
    21061 ttagtatttt ttatacctgt agtaattggt atgggtggaa atattggaac tcaatcatca
    21121 tctgttacag taataacatt atctaataag gatttaaatt ttagcaatgt ggttagagaa
    21181 ggtattgtag gtattattac aggtcttttg tgtagtataa taacaggtat tgtaatttac
    21241 tttgttatga gaaagttaga cattgtatta attgtttcaa tatcattatt tataaatatg
    21301 gttttaggtg caacaatagg tgcgtttatg cctgttttat taaaaaaaat ggatgcagac
    21361 ccatctacag tttcatcacc aattatatct acagctcttg atataactgg tatagcagtt
    21421 tactttataa taacaacggc attattgtca aaaattgttt aataacttta tgtatgattt
    21481 atcctcttct tactaaaaat ataagtataa atgtaagaag ggggtttttt aaaaatgaac
    21541 aatatcagtt gggaagtttt taaaaaaaca ggtagtatag atgcatattt atatttctgt
    21601 gattgcaaaa acatacatga agaaattgaa gaagtaaggg agaaaaaaga agacgatgat
    21661 aatcctgaac acccagggga tagtacttaa agctataagg tataaggaaa gtgacataat
    21721 acttacacta tttacaagaa agcttggaaa agtatctgca atagcaaaag gagctaaaaa
    21781 aaataagagc tctttgcttt catcatcaca attattttct tatagcaatt ttaccttaaa
    21841 aaaacaaggt aatatgtaca aggttactca aagtgaaata ataaaaagtt tttataatat
    21901 atcctatgat atagaggcat tttcttatgc tacatatatt acaaaattag ttgaaaattc
    21961 tatacttgaa aatcagacta ataataggct atttatatta cttgcgcaga ctctgtattt
    22021 gtatacacag gataatacag acaataggtt tataacagca gcattcgagt taaaattttt
    22081 agactatata ggatttaaac ctatagtaaa taaatgcatt aattgtgaat gtaaaatttt
    22141 acaaaattct gtgtttaata tctacgaagg tggtatacta tgtagtaagt gtagtaaact
    22201 ttttgataat aatataaaat tagacttaac aactataagt cttatggaat atgtattaag
    22261 aaatgatatt ttaacatgta gtaaagctaa agtatcaaaa tatataacac atgaacttga
    22321 gaatattcta gacaagtatt tgaaagttta tgttgataat attaatttta aatcattaca
    22381 cgtattgcaa agtgtaaaaa ataataaggg agtggataat gatgagtagt aatataacta
    22441 ttgaaatggt tgattcagtc tttgaaagag ttccaggagc aacttatgca gaagcaaaag
    22501 aagcattagt aatgtgcaat ggagatgtaa ttgaggcagt aatatattta gaaaataaaa
    22561 aaaatacttg taattgtaaa gcaaaatctg ctaaagaaac tatggaagaa gtatttggca
    22621 aagatggaga aaatataaag tttcaattaa aagagcttct tagaaaaagt agtgtagtga
    22681 gaataatagt tgaaaaagat ggaaaaacta tgatgaacat tcctttaaca gttggagttg
    22741 ttggattagc ttttgtacca ttagctactt tagttggatt atcagctgct gttataagta
    22801 aatatcgtat aaaagttcaa aatgaagaag atggagaaat tgtagattta ggtgaattga
    22861 atgaagaaaa acttcacgtt ttgaagaata tgataactaa tgcagcaaaa gatgtgaagg
    22921 atgtagtagt tgacaaaaaa gaagatgaca aagatgtaac tgctgacctt atgaaagaag
    22981 aatctgaaaa gaataaagaa gacgaagacg aaaataaata gatataaagt gacttttaaa
    23041 aaatgaagag aagataatta taagaattat cactaacatt gacaaatttt atagaatttg
    23101 atattctaaa agttgtagaa gaactattaa aatagccttt cgtgtagacg agaggcttta
    23161 ttgttatagg agggattttt atgaattttc aagagatgat actggcgttg caaaaatact
    23221 ggtcaaagca aggttgtatc atgatgcaac cttatgatat tgaaaaaggt gcaggaacta
    23281 tgaatcctaa tactttttta agatcattag gacctgagcc ttggcaagta tgctatgtag
    23341 agccttcaag aagaccagca gatggaagat atggtgaaaa cccaaacaga ctatatcaac
    23401 atcatcaatt ccaagttata ctaaaacctt ctccagataa tatacaagaa ttatatttgg
    23461 aaagtttaaa agagattgga attgacccaa gtgagcatga cataagattt gttgaagata
    23521 actgggaagc tgctacagtt ggtgcttggg gacttggatg ggaagtttgg ctagatggta
    23581 tggaaataac tcaatttacg tattttcaac aagtaggtaa tattgaatgt gagcttgaga
    23641 caggagaaat tacatatgga ttagaaagat tagctatgta cattcaagaa gttgatagtg
    23701 tttatgattt aaaatggaat gataaaataa cttatggtga agtatttaat aaagctgagt
    23761 atgaaaactc aatgtacgca tttgaattat gtgatgcaga tatgttgttt aatctgtttg
    23821 atatatatga aaaagaagca ttaagactta tggaaaatgg ccttgtaata ccttcatatg
    23881 actatgtgtt aaaatgctct catgcattta atacattgga cgcaagaggt gcaataggag
    23941 ttagtcaaag agcttcattt ataggtagag ttagaaatat ggcaaagact gttgcagaaa
    24001 cttttgtaaa acaaagagaa gaaatgggat tccctctttt aaaggacggt gacaaataat
    24061 gaaaaattat cttttatttg aaatcggtgt tgaagaaatg ccatcaagat ttgtaggcag
    24121 tacactagaa caattaaaaa acaatcttag taaattattt gatgaaaata gaatagcttt
    24181 tgataaaata aatgcgtatg ggacaccaag aagacttact ttagtagtcg aaggattgag
    24241 tgatagacaa agtgatttag aagaggaaat aaagggtcct gctaagaaaa ttgctgttga
    24301 tgcagaaaat aatttaacaa aaccagcatt aggatttata aaaagcaaag gattaagcga
    24361 atctgacata tattttaaac aagttggaaa agaagaatat atatttgcga ctataaagca
    24421 agatggaaaa gaaacttcag aagtactaaa agacatatta ccagagacta taaaatctgt
    24481 agtattccca aaagctatgc gttggggagg aaaaaatctt aaatttgcaa gacctataag
    24541 atggatagtt gcattgttaa atgataaagt attagagttt gatttagaag gaataatatc
    24601 ttctaatgta acaaaaggtc atagattttt aggtgaaagt acaatagaag ttgattctat
    24661 agaagattat tttgaaaaat tagaaaaaaa ttatatagtt ttagaccaaa acaagagaaa
    24721 agaaatgata agaaaacaat gtatagaagt agcaaattct ttaggtggag aagttgaatt
    24781 tgatgaagat ttacttgaag aggtaactta tttagttgaa tatccaactg ctttttatgg
    24841 agaatttgat gtggattata taaaactacc aaaagaagtt gttataactc ctatgaaaca
    24901 acatcaaaga tattttccag tacttaaaga aggaaaactg ttacctaact ttatagctgt
    24961 tagaaatggt gattcttaca gaatagacaa tgttaaagct ggaaatgaaa aagttttaga
    25021 agcaagactt gcagatgctt tatttttcta tagagaagat actaagagaa acttagaaag
    25081 ctacatagaa aaattaaaaa ctgttgtatt ccaagtaaaa ctaggaacta tttacgacaa
    25141 aactttaaga cttgaaaaat taagtgctga tatacttgat agtttaggtt tagtagatga
    25201 aaaggcagat actataaggg ctgctaaatt atctaaggca gatttagtta ctggaatggt
    25261 aggagaattt gatgaattac aaggttttat gggaaaagaa tacgcaaaag ttggaggaga
    25321 gaatgatgtt gtaagtgaag ctatttttga acattatctt ccaagatttg caggagatat
    25381 acttcctaag actaatgcag gggttgcctt atcaatagct gataagttgg attcaatagc
    25441 aggtttcttt gctataggaa tacaaccaac aggttctcaa gacccatatg ctttaagaag
    25501 acaagcttta ggtatactta gtatacttat ggacagaaaa gttgctgtgg atataaaagt
    25561 acttattgaa catgctttag ataattattc tgaattagat tttaataaag aagaagttgt
    25621 tgagcaaata atgaatttct tcaatgagag agtaaaaaat ctatttaaag atttaggaat
    25681 aagatatgat gttgttgatg ctgttttaag ttctgatata aatgatgttt ctgatatgca
    25741 tatgagagct ttagagctta ataattggct agaaaaagat gaattagtgg agatgcttac
    25801 tgcatttaat agagtttcta ctctagctca aaaagcagaa agtgataaaa ttgatgaatc
    25861 tttattaaaa gaagatgctg aaattagact ttacaatgaa tttaaacaag taaaggtaaa
    25921 agttaatgaa ttattaaagg ataagaaata tggtattgct ttagatgaat ttgcatcttt
    25981 aagaccagta gttgataatt tatttgatac agttatggtt atggataatg atgaagctat
    26041 taagaacaat agacttgctt tacttaaaca agtatatgat actatgctag aaatttgtga
    26101 tttatctaaa attgtttata aataaaattt aaactattta taagaaataa ggctgtctca
    26161 aaatagagat agtcttattt ttcattgttg agatgatgaa aatatatttg atttcataga
    26221 aaaagagtgt ctcataaact aaaaagttca ttttgagaca cccatgcttt ttgtaagtaa
    26281 atataataaa ttatatttta tacttatcag ttattttgtt taaaatatta tcatttgaat
    26341 ttacccattc ataaaaattg cctgatgatg cgtccgatat tttataaagc atttcaggaa
    26401 tatcactagc tttaaaatct ccaagtgatt ttcccaatct agtcttacct atttcaaaac
    26461 tgcctccaac aaataattca aatgcatctg tagatatacc atcaacattc ttctttttac
    26521 cacataaacc tatgctacca acttgatgaa caccacatga atttaaacat cctgatatat
    26581 ataacttagg tgctttattt aaaatttctt cattattttg taatctaaaa taatctatga
    26641 tttcatgtaa cattttctga ctattttgta tgcccatttg acaaattgga acacctatgc
    26701 aagatatact ttgttctaat tgagaattac aactaatagt tttacttatt tctaatattc
    26761 tttttgcttc atttccattt aaatttaaaa tatataatcc ctctgtcatt cctagtctaa
    26821 tcataggatt ttcaacatta tctaattctt taagtaaagt acttaaatcc tttgttaaca
    26881 aaatacctcc aactggatgt atatatacag tgtataaacc atcctgtttt tgaggtatta
    26941 gtctagaatc ttgtatatct attttaactc caggtttaga gtaatctatt tcttcaatat
    27001 tgagttcaag actaccattt tctttttcta atttatagta ttctttaaat ttttctaaga
    27061 atagttcttc accaagtgat tcgaccatat atctaaccct agctctgttt ttattttcat
    27121 aatttccata atccataaag aatttaatca taccttcaac acaatataag gcgtctttcg
    27181 gctttataag ttcatcaagt tctaaagcaa ctttgggttg tttacctaga cctccaccta
    27241 agaaaactct gaaatatggt tcatcatttt tcaaagtagc aacaaatcct aagtcttgta
    27301 tactacaatg agcagtatct gtgtaacaat tagaataaga aacttttaat tttctaggta
    27361 aatggtatgt agtagcattt ttgataaaat atttatcagt agcaactgca tatggagtaa
    27421 catcaaagac ttcactaggg tcaactccag ataatggaga taatccgaca tttctaggat
    27481 aattaccacc accacctcta gtgaatatat cattttttat tccttcttcc atgatatcaa
    27541 caattgaatt tatagataaa tcgtgtaatt gtatagcttg tctagttgtt aagtgtatct
    27601 tatctaagtt gtgtttaata gctatttcat agattttatt aagttgatac tgagataaaa
    27661 ctccagcaga tactctaagt cttatcataa aagatttttt gtctctttga gcataaacac
    27721 catatccacc agaaaatccc ttatattgca ttttatttat ttctccattt aaaaattttg
    27781 aagaatattc tttaaaatta ggtagttcat ttaacaatat ctttttaaaa ttatccatta
    27841 tttcacctca ttgttagtct tgtataaaaa tatatctatt gtataataga tatatatatt
    27901 attatctagt caggataaaa atataacaga ataattctat taaaagtaag aataataagg
    27961 acatagtgag tattttgttt ttattttaca aattaagtat aatatttttg ttttataaaa
    28021 tataattaag ataaatagat aattttgtaa ttttaatata taatatttag tagagtgtgg
    28081 gagtagcact taatatttgc aggaggagtt tttgtatatg ggagaaataa taaatgcatt
    28141 agatagagcc ctagacataa ttttgctgtt atatcatgaa aagagagaaa tgggaataac
    28201 tgaaataagt aaggctatgg gagtatataa aagtactgta catagaactt tggttactct
    28261 tgaaaacaaa ggttttgtta tacaaaatgc agagaatggt aagtattggc taggaataaa
    28321 tttatatgca ataggtatgg ttgtgggaga aaaaatgtca ttgactgaaa tagtaaaacc
    28381 atatacgaaa aaattaaatc aagaatttaa cgaagtagtt aatgtatcta tattagaaga
    28441 aagagctcaa gattctccaa gaagtatcat aatacataaa gagtatggtt caaatcaatt
    28501 actttcagta aatccatccg ttggttcaag tagtgaatgt tattgttcag cagttggtaa
    28561 atgtcttatg gcatttaatg attctataga ttttgaaaaa tatagaaaaa caccaataca
    28621 caagtataca gaacatacaa ttgataattg ggatgatatg atgctttttt tagcaaaaat
    28681 aaaagaacaa ggatatgcta tagatgatga agaactagaa catggactaa cttgtattgg
    28741 agcacctatc ttagataaaa ataataaagc tattgcagcc ataagtttgt caggtccaac
    28801 tataagaatg agagaagggg actttgagta taagattaaa agagttatag aaactgcaaa
    28861 aagtatatct gaacttttta gatgattttt aatctcgata ataaaaatag gctatattta
    28921 aattgttatt tgtaaatatc agtttaaatg tagcctattt ttctttaatc taaaattaaa
    28981 tattcttaaa atttaaaatg ttattgaaat atataagcga atgtgttatc ttttaattaa
    29041 gcaataaaaa tagaacaagg ttctatacta tagaacaaaa ttgatgcata tatagtattt
    29101 atacaaaata taaatattat atatttaaaa ttaagtaaaa atattacata aaggggagat
    29161 ttgagaaatg gaaaaagtca agttgagtgt tgaaagatgt aagggatgct atctttgtat
    29221 tgaagcttgc aaaaaagatg caataagtct ttctgactac acaaataaaa aaggctttgt
    29281 aactattaaa gtcgatgaag aaaaatgtat tcaatgtggc gcttgttata caatgtgccc
    29341 tgatttagta tttgagatat tataaattat ttttattaga ggggagaaaa ggtatgcaaa
    29401 gtttattaat taaaggaaat gaagctttag cagaaggtgc tatacgtggt ggctgcagat
    29461 tttatgcagg ttatccaata acaccacaat cagaaatatt agaaatgatg gcatggagac
    29521 aaaaagaagt tggaggagta tttatccaag gtgaatcaga aatagccagt gtaaatatgg
    29581 ttatgggagc aagtgcactt ggagcaagag ctatgacttc ttcatctggt ccaggatttt
    29641 cattaaaaca agaaggtatt gcatattggg tatcagctgg tttgccagca gttgtagcat
    29701 gtgtgcaaag atatggtata ggtgatggat ttataggagc tggtcaagat aattattggc
    29761 aagcagtaaa aggcggtgga aatggagatt atcatttaat cgtgttagcg cctaacagtg
    29821 ttcaagaaaa tatggatatg gcatatgaat cttttgaatt ggctgaaaaa tatatgcatc
    29881 cagtacttat actttctgat gctacaatag gtcaaatggt agaaccttgt atagcaccac
    29941 caatgaaaga atacgatatg gataaagctc catgggctgc aaaaggaaca cctgtaggag
    30001 aaaaaaataa agtaataact gatataacat attttgaaga ccaagataca tggcaaaaag
    30061 ggtatataga aaaaatgaga aaagttcaag aagatgaaca aagatgggaa gagatagaaa
    30121 tagaagatgc tgaagtggta tttgtggcat acggtatttc atcaagaata gcagaaggtg
    30181 cagttagaga tgctagagca aaaggaatga aattaggtct aataagacca aaaacattgt
    30241 ggccttaccc aagaaaagca tttaagaaat taagtgattc attgaaggga cttgtaacag
    30301 ttgaaatgaa catgatggca cagatgaagg aagatgtaat tatagcatgt gataatagat
    30361 ttccagtata ttcagtagca acagctcaat atatggcaga agtaaatcaa ttaatagatt
    30421 ttgctcaaca gattatagat ggaaaagcta agcaagagga ggtattttaa ttatggctaa
    30481 gaaagcacca gcaataatga tgtgtgaaaa tatgttttgt ggaggctgtg gacactctat
    30541 agtcgataga attattggag aagtattaac agaaatggat atatgtcaag atacaattat
    30601 atgttcagat ataggttgta atcatatgtt tcaatttagt atgaatgtag atgttgtcgt
    30661 tccacctcat ggtaaaatgg gtgcagcaat ggcagctatg aaaagagtta gaccagataa
    30721 atatatattt acaatagctg gagatggtgg agcttatgcg atagggcttg gggaaactat
    30781 gtcagctgct actagaaatg ataatgttat attccttgtt atgaacaata atatgtttgc
    30841 aatgacagga gggcaaatgg ctccaactac tatagaaggt caatatacag cttctactcc
    30901 agcgggtaga aattacgcag atcatggttc aacatttgat gttgtaaaag ttatgggaaa
    30961 tttagatata gcatatctag caagaggaac aataactaac cctaaagaaa taaatacaac
    31021 aaaaagatac atcaaaaaag ctctagaaaa acaaaaagaa aagaaaggtt tttgcttaat
    31081 agaagtgctt gtaccatgtc caacaaactg gggattaaag ccagtagatg ctatgaagag
    31141 aattgaaaat gaacttgttc caaagtatcc acttggagaa tttattgatt agggaggaaa
    31201 taatatgttg aatgaaatta tatgtgctgg ttttggagga cagggagttc ttacagcagg
    31261 aaaaattata atgtatgttg catataaggc agaaaagaaa attacatggt tcccatctta
    31321 tggaaatgag atgaggggag gaagtgcaaa ttgcaatgtt gttgtaagtg atgaaaaaat
    31381 tgcaaatcct tgtgtaaaaa atccagatat actattggga atgaacagcg tatcagtaga
    31441 taagtatgaa agtttaatga aaaaaaatgg ttatatgttt gtcaatgaat cactagtaag
    31501 taaggataga aagtacagag aagacattaa tgttataaaa gtacctgtta ctgaaatagc
    31561 acaaaattta ggcaatgaaa aagctgctaa catttgcatg ttaggtgcta tggtaaagag
    31621 tttggattta tttacaaaag aagaatttga aagtggtatg tgtgaatact ttgaaaatca
    31681 aggcaaaggt aaattcaatt ctaaaaatgt agaagcattt gaagcaggat acgactacga
    31741 atgaatattt taaatattta aaataattat tgaaatactt atttttatat gttagtattt
    31801 aattaataaa atatagaacg tcgttctgga caataggacg aagtctttag gccggaatac
    31861 aagaacgacg ttctgtatag tagaactagg gggtgttaaa ttgaaagttt tagtaataaa
    31921 tcctggtggt acatccacaa aaatttctgt tttccaagat gaaaatgaaa ttttgaaaaa
    31981 aaatataacc catacaagag aagatttaaa aaatttttct aaagtatttg atgaatatga
    32041 ttatagaaaa caactgatag tagatatttt aagtagtgag aatcatagta taaattcttt
    32101 tgacgcagta gttggtcgtg gtggacttat gaaggctata aaaggtggta cctatactgt
    32161 gagtgaagaa atgattgagg atatgagaaa tgaaataaat ggggaacatg catcaaattt
    32221 aggtgcatta ttagctaaaa ctatagctga tgaaatagga gtacaatcat ttgtagtaga
    32281 cccagtttct gtagatgaat ttgatgatgt atcgaggatt acaggaatat cagacataga
    32341 aaaagcaagt tggttacatg cattaaacca taaagctgtt tcaagaaaaa ttgcacaaaa
    32401 actaggggga aaatacgagg attataactt tattgtagct catttaggtt caggaatatc
    32461 gattgtagca cataaacaag gcaaaatgat tgatggaagt ggtggtaggt ctgatggtcc
    32521 attttctcca gaaaggagtg gtggattact tgcatatcct cttatagaat tgtgttactc
    32581 cggaaaatat actcatgatg aaatggtcgc aaaagtaagt tcgattgggg gaatgtacga
    32641 ttatttagga acaaaggaca tgatagaaat tgaaaacaga atcaaggagg gtgatgaaaa
    32701 ggcaaaactt ataatggatg catttattta tcaagtagca aaagaaattt ctatgtatgg
    32761 agcttcactt tcagggaatg tagatagagt tatcttgaca ggagggatat ctcactcaga
    32821 aatggtagtc aaaggagtag cagaaaaagt ttcatatcta gctccaatag agatagtacc
    32881 aggagagatg gaaatggagg cacttgctct aggagcactt agagtactta atggagaaga
    32941 aatagcaaaa acttattaat atttaggaga aagagtatgt taaaagattt taataattta
    33001 ttagaagagg ctaaaaagct tgaacctgtt attgtaagtg ttgctgttgc agaagacaaa
    33061 gaagtattaa aggctgtgaa atcagcaaca gatttaaaca ttataaagcc aattcttgta
    33121 ggagacaagg ataaaataga aagtatatca agagaaattg gattaaaagg tcatgaaata
    33181 ataggttgtc aaaatcccga tgaatcagtt aaaaaagctg tagagatagt taaaaataag
    33241 gaagctaaag ttcttatgaa gggattagta aatacaagtg tctatatgag agctatatta
    33301 aataaagaaa cgggtcttag aacaggaaga ttattaagtt tgcttgcagt atatgaatta
    33361 cctcaatatc ataaacttct ttattgtact gatagtggaa taaatgtatc gccaaactta
    33421 aatcaaaaaa aagatataat gacaaatgca cttcttacta tgaaaagtat aggcatggaa
    33481 aatcctaaaa ctgcgatact tactgcaaat gaaatggtag accctaagat acaatctaca
    33541 gtggatgcca acctacttgt agagatggaa aaatcgggag aaataccaaa gtgtatagtg
    33601 gaaggtccaa tagcatttga tgtagcattt gatgcacatg cagcagaaca taaaggtatt
    33661 aaaagtaata ttgctggtga tgttgattta ttagtatttc caaatataga agcagggaat
    33721 atattaggaa agtcatggct tagatttaat aaagctaagt gggcaggtat agttttagga
    33781 gcaacaaatc cgataatttt aggttcacgt tctgacacag ctgaaataaa aataaattca
    33841 atagcattag cgtgcctagc atcacaataa gaaattatta ttaaggagga gaaagtatta
    33901 tgaaatatgc aaaaagaatg gatatggtaa aagcttcagc tataagagct tctcaaaaga
    33961 aaatagctca gaaggtggca agtggtggaa ctgttatatc ttttgctgca gggcttcctg
    34021 acccaaattt atttccaata gaagagataa gtgaaataac tcaaaaagta cttaatgaaa
    34081 ggggaaaatt tgccctagca tatggaccta ctaaaggtga agatgagttg ttagatctat
    34141 tagtaaaaag aatgaaagaa gaagaaaata ttgatacaaa agcagaaaat attataataa
    34201 ctactgggtc acaacaaggg atagcattaa gtgctatgat tttactagaa aaaggagata
    34261 tagtagttac agaaaatcca agttacttag gtgcaataaa tgcatttaga ccttatgaat
    34321 gtgatttttt aggagtagat actgatgatg aaggaatggt aacagatgat ttagataaat
    34381 tattatctga aaattctaac ataaaggtaa tctatgtaat accaacattt caaaatccta
    34441 caggaaaaac atggtcttta caaagaagaa aagattttat ggaagtcgtg aataagtatg
    34501 atgtcatagt aatagaagat aatccttatg gagaaataag atttacacca gagcctctac
    34561 ctacacttaa atctcttgat actaaagata aagtattata cttaggttca ttttcaaaag
    34621 tattatgtcc agggtttaga gttgcttgga tgtgtggtaa tgtagacatc atagataagg
    34681 cagaactttt aaaacaaggt atagatttac agtcaaatca attttcgcaa gtacaagtaa
    34741 tagaattttt aaagaaatac aacttaaatg aacatataga aaaaataaga gcagagtata
    34801 agaaaagatg tctgctaatg ttagatgcta tgaagaaaca tttcccagag gaagctaaat
    34861 atacagaacc agatggagga atgtttatat gggttgaact tcctgaaagt ataaatgtag
    34921 atgatttatt ggataaagcc attgatgctg gagttgctta tgtttctgga gagtccttct
    34981 ttgcaaataa tggtccaaaa aatactatga gacttaacta tacaacaatg tcagaagaac
    35041 aaatcgttaa aggtgttgag atacttgcag aagttataaa aaaagaactc aattatgctt
    35101 gtgaataaat ataaaaagtg agggtttaaa tatgggagaa aaagaaaacg gtataaagtt
    35161 tggaaagtta atgtttcaac ttgttattat agcaatatct tgggaattag tttatattat
    35221 accatttatc caatatacat tatatgatcc aatcctaaaa gcattagaat gtacaaacac
    35281 tcaactaggt tttctactta caatttatgg gttaggtaat atatttggag ctcctatagg
    35341 aggctggtta gcagatagat ttgattatag aaagataatt ttaggttctg tatttcttaa
    35401 tggagtagta tcatttttat ttgcatttaa tatgaactat ccatttgcag ttgctacttg
    35461 ggtaggttgt gcaatcacat cattagttat gaactatcct tctatggtaa aaatacttag
    35521 agttatagga aaagataatc aaggtaaagt ttatggattc aatgaagcta tggttggagt
    35581 ttctggcgta gtaatgggag caatattctt atatatttat acatgctttt caactcctgt
    35641 attgggtatg agatgggtaa tgatatcttt aggaatatta tcaatagtga tgtgtccagt
    35701 attatggttt gtaattaaag atgtagatat cagagaagac aaagaagaag aagcaagtga
    35761 aaaaatgtct gctggcgatt ttatgacagt actaaaatca ccaaacactt ggctagtagg
    35821 aataagcata ttctgtgtat attcattcac agttacaatg tcttatttta caccatatat
    35881 aacatcagta cttggaggtt ctgtggcatt gtctggggca ttagcaataa taagacaaca
    35941 tggtctaaaa ttgtttggag caccatttgg aggatattgt gcagataaag taaaatcacc
    36001 tacaaaagta ctactaccaa tatatgtatt tggtatagca gtaataattt tattcctagt
    36061 actacctgca tctactccaa tgactatctt tatagcttta acatttgtag taggtatatt
    36121 aggttacatg ggaaaaggta tatactatgc agttcaagat gaagtaaaag ttccagttaa
    36181 atattcagca actactatag gtatagctgc agctttagga ttttcaccag atgtattcca
    36241 atttgcatta ataggacact ggatagatac ttatggcaat aaaggttaca catatacatt
    36301 tatattccag atagcaattc taataattgg tatactatct tgtttatata tattaaaagt
    36361 taagaagaaa agagaaagta agttagaagc ataaatattg taaaaatacc aaattattaa
    36421 gttttatagc aatacttaaa atttaaagtg caacacacca aaatgttatc aagttttcta
    36481 ggttttagta taaagttacc cctaaaggtc tggaattgag tagcaattat catctattta
    36541 tatatttaca taaacctatt taatttaaat taaaagaatt tcaaaaatag gtggtaattg
    36601 tactcaattt tacacctcat aataaatacg aaaatttaaa atctaactat atactattca
    36661 attatatatt aataattgta tatggtacta tttatatagt aacagtttca ggcttttcat
    36721 tacatgtatt ccaatttata ttgtttgatt atttgtcaaa tacatatcgt taaaaagttt
    36781 ataaatttac gtttatatat cgattaatat tctttgtttt agagatagat acttatagct
    36841 tgtggattaa aaataatata aatagaagat agatttttta gatatattga gttgaagatt
    36901 atatagaatg ttgaaacata gctatttaaa ttattgtcta taaatataaa atgaatggtg
    36961 gtgagttaag tgaccgattt agttaacacc attaatggaa ttgtttggtc acctgtatta
    37021 gttgcaatgt gtttgtgtat aggattatat ttttcaatga aattaagatt ttttcaaata
    37081 aaagatgtaa aagaaatgtt tactcttttg cttgaaggag atagctctca tgttggaata
    37141 tcttcgttcc aaggttttgc tacatctatg gcaggaagga taggaactgg aaatatagct
    37201 ggtgtagctg tagcgatagc aatgggaggg ccaggagcat tagtgtggat gtgcataatg
    37261 gctacattag ggtcggcaac tgcatttgta gaatctactt tagctcagat atataaagat
    37321 gagcatgatg gacagtacag gggaggtcct ccatattact ttgaaaaagg tcttggttgg
    37381 aaaaaatgtt caattttatt ttcagtggtt gcaatcataa gttatacatt ttttatgcct
    37441 ggcacacaat caaatactct atatctagca atgaatcaag catttggatt ttcaaaaatg
    37501 acaatagcag ttacatcttc tattgcacta ggtataataa tttttggagg aattaaaaga
    37561 attggtttat ttgctgagaa agtagtacct ataatgggtg gagcatacct tattatgaca
    37621 ttggtaataa tcatagcaaa tataaatcta gttccaggag ctattatgac aatgtttaaa
    37681 tctgcatttg gatttggttc ggtttttgga gggatgttag gttcagcaat atcttgggga
    37741 gtaaaaagag gtatatactc aaatgaagcg ggagaaggtt ctggaactta tggtgcagca
    37801 gcagcagaag tgagtcaccc agccaaacaa ggattagtcc aagctttttc tgtttatgta
    37861 gacacactat taatatgtat ggctacaggt attatgatta taataacagg aatgtataat
    37921 gttacagatg tttacacaac attaccaggt gtagaaccag gtccacaata tgtacagagt
    37981 gcaataaata cattacatcc tcaattaggt gatattttta tagcaatagt tttactacta
    38041 tttacattta caacattatt agccttctca tacatgatgg agaccaacgt atcttactta
    38101 gtgactagaa ttaaggcaga aaaacatgaa aagcacataa ttaatgtctg tagatttatg
    38161 cttataatat gtgcagcaat gtgttgcttt atagaaccac ttacaagttg ggcacttggt
    38221 gatatatgta taggtgtatt aacatatata aacttagttg gtatatttac actaaaaaaa
    38281 ccagctataa aagcatatta cgattatcaa aggcagaaaa agatgggagt aaaaaggagc
    38341 gatagaactt ttaatcctat agaattagat ataagaaatg ctgagttttg ggaaaataga
    38401 tacttaaaag aggataagca tgagttaaat taaagttata ataaaatata aaaattacta
    38461 aataaattta gtgggaggta agattggtat gaaagaaaga ataatacaag tggctgattc
    38521 aaagaaagaa aaaattctag ggctatgtca atctttatat gatgaaccag aaattgcttt
    38581 gcaagaatat aaatctgcta aaaaaatatc agaatttcta agagaagagg gttttgatgt
    38641 tgaggaaaac ttggcaggta tggctacagc ttttaaagca accaagaaaa atggagatgg
    38701 tcctaaaata gcatttatag cagaatatga tgctcttcca ggcaatggac atgcatgtgg
    38761 acatcattta attgcatcta tgggagttgg agctggaata gctctttcaa gtatactgga
    38821 tacttacaag ggagaagtaa gtataattgg aacacctgct gaagaaactg gagatggaaa
    38881 gccttacttg atagaacatg gtgtatttga tggctatgat gcagcaatga tgatacatcc
    38941 aaattcaaaa acttgtgtaa cacctgaaat tatagcaata ggaggtctag atttcatatt
    39001 tactggaaaa gcatctcatg caggagctaa gccatataac ggaataaatg ctttagatgc
    39061 agtagtacta ctatataata atataaatgc actaagacaa caattagtag atggaacgag
    39121 aatacatggt attatactag aagcaggaac tgctgcaaat gtaatacctg atatgggaaa
    39181 agtaagatta gagataaggg caaaagaaca aaattacttt gatgaagtag tagaaaaagt
    39241 gaaaaattgt gctagaggtg ctgcaatagc cactggatgt gaacttgaat tttatcactt
    39301 tgaaccaact tgccaaggat taaatgaaaa taaagttttg gtagacatat ttacaaagat
    39361 tatggaggaa tttggaatat atgaagatga acaagtaatg ttaggttcaa cagatatggg
    39421 aaatttaagt caaataatgc catgtataca tcctttgatg aagttttctg aaaatggaga
    39481 agaactacat acaaaagaat ttttggaggc ttctataaat tcatatgcaa aagatagagt
    39541 aattgatggt ataaaaatat tagctttaac aggatttaat ttatttgaaa atccagagtt
    39601 attgaaaaaa atgaaggaag agtagaactt agactatttt taattatact ctttaaaata
    39661 agaagagtgt ctcaaaatga actttttagt ttatgagaca ctcttttttg catgaaatta
    39721 aatatatttt tattatttta acaatgaaaa agaggcttat ctgtttttat ataacctttt
    39781 ttaacttgta gcacaaaata tcatgaatga ttacaattcg aatgataaaa tcgattataa
    39841 gatatatagg aggggtacat atggaaactt tagaagtaag aaatattgaa ttgagtggta
    39901 caaattatga gataggatac agactaggag aattggttgc taatatgcca gagataatag
    39961 aaggtcagat taataaatct aatctggtaa gcaaaaaaga agaaaaagag atgatagagt
    40021 tgtttgataa atattgtcca ggtcttaatg aagaattgca aggttttgct gatgcaattc
    40081 aagtgaattg taatcaaatt ttatattata ctatgactta tcttaaacca ggttgtagtc
    40141 aagttgcatt aacaccagaa ttaactgaaa atgggcatgt actatttgca cgtaatttcg
    40201 atttctcaca caatatggag gattttgttt tatgtaaaac taaagtaaat ggaaaatatg
    40261 cacatatagg tactaccgtt atgcaatttg gaagaggtga aggtatgaat gaatgtggat
    40321 taggtgtaag tcagtcatca tgtggtattc ctgtaggcaa ttcagatggt ttgagaaaac
    40381 cagcaattgt aggtttacag ttttgggctg ttattagata tttgttagaa aattgcaaag
    40441 atgtagatga agcattagaa tatttaaaag atatgccaat tgcatacaat attaatttat
    40501 tgcttgcaga taagtctgga aacattgcac ttgtggagac tttagatggg aaaaaagaag
    40561 taaatataat caacagttta gaaagtaaga gagaatattt tttacactct actaatcata
    40621 cacatattga taaacttcat aaattagaac cacaatcaat gaaaaattct attcatagat
    40681 ataaattaat taaagaatat ataaataagg ctaaaaaaat tggagagaaa gagttaatga
    40741 atttattgtc tttaaagtat ccaaatggct tatcttgtaa ttactataat gatttttttg
    40801 gaactttaaa aagtatagtt atggatttaa atataggaaa gtttaatatt ttatggggtg
    40861 gactagagaa taaatgggag agctactatt taaaaaatga cattaaatct ataacacaga
    40921 ggataaacat aaatattgaa aaagcaccat ctgacttttt tgatttcatt gaataaaaaa
    40981 tgtatttaat tattctaaat aattaggcta tataaatcaa ctaaaagttt ttatttatcg
    41041 tattgaatta gttataatat tttagtatag taaaaaatag tgctatttta catatttaaa
    41101 gagtttaagt agaagagagg tgccatattg gattacttaa agtattttga aaaagctgta
    41161 atgtatattg agaataattt acatgaaaat ataactgtaa atgatgtggc aaaagaaact
    41221 ggatattctt attatcattt aacacgctta tttaagtcta tgtttggtga gagtgtagga
    41281 agttatataa aaaaaagacg attagtaagt agtacaaaag aacttttata tagtgataaa
    41341 aaagtgatag atatagctat tggtagtgga tttgaatctt cggaagcatt tagcagagca
    41401 tttaaatcta tatacaaggt tagccctata gaatatagaa aaaatagaat tgatgtattt
    41461 gttggtaaaa agaaaaaatt agagttagat tttatgagac atcttgttgg aaatataacg
    41521 attaaaccag ttataaaaga aatagaaaaa ataaaggtaa taggtataaa ggataaagta
    41581 attttagaaa ataattcatt acctgacttg tgggagaaat tcagaaaagt acatcatatt
    41641 gtcccaaata ctttgccatc aaagagagtt tttggaatct gtgaagcaat ctctgaaata
    41701 cacctagtta gtgaaagtat ggaatttaat gagataatag gacttgaagt taatagttat
    41761 gatgtgattc caaattcatt tgtttcaaaa actattaagg aaggaaagta tgctatattt
    41821 actcatactg gtagcttaga taacttagac aaaacttatg aatatatatg gggtacttgg
    41881 tttttaagtt caaaagaaga actggatata agagatgatt ttgaagtata tgatgaacga
    41941 tttttaggtc ctagtaatgt gaactcacaa attgatattt atatacctat aaaataatct
    42001 ataagaaaac taaaagataa tttgattaag aaatagatgt tcgtaaaaga gcatctattt
    42061 tttattttta tcagatttag ataattttaa taataaaaga aataaaaata agttcttaaa
    42121 tttctaagaa gaatttagaa tatagtcgaa ataagaattg tatatttctg aaaattctaa
    42181 ttagtacaat tttttccttt ataaaattga aaaaagatgt atttatttat caaaaatatt
    42241 atattaaaat gatattaata aaaaaacatt tacaatatta gcatatagta cagaaaaaag
    42301 atgtaatttg taaaatttat gttaagcagt aaaattaaaa atttaaagat attataggga
    42361 ggttataatt ttatggaaaa cgttattaca ggtctaagta aaggtgcaga gtggtttatc
    42421 ggtttgtttc agaaaggggg agaacaattt ataggtcttg tttcaggtac tcttccaacg
    42481 ctaattgtat taatggtagc tattaactct ttgattaaga taattggtga ggaaagagtt
    42541 aataattggg cttctaagct aggaaagaac tcttttacaa gatatattct acttccatta
    42601 gtatcagtat ttttcttggg aaatcctatg tgctatacat ttggtagatt tttaaaagaa
    42661 gagcacaaag caggatttta tgatgcagca gtttcatttg tacatccaat tacaggtctt
    42721 tttccacatg caaatgcagg agaacttttt gtatggttgg gaatatcagc aggtttgact
    42781 actttaggta aagaaactac tacactggca ttgtggtatt ttatagttgg tctaattgta
    42841 atttttataa gaggtatagt aacagaaaaa atgtatgctt ttctaacaag aaaaaataga
    42901 gcaacaaata aggcttagga ggtagataaa atggaggaga ataaaatatt aaaaatagaa
    42961 aagggaactt caggctgggg aggtcctttg tatataaaaa aagaagggaa tagaaataag
    43021 atactatcaa tgactgctgg tggtatccat gaagtaacat taaaaataaa agaactactg
    43081 ggttgtgaaa ttgtagatgg atttaaaact ggagtttctg atgaagaagt agcagtagtt
    43141 attattgatt gtggtggaac tgctagatgt ggagtttatc caaagaaaaa aatacctaca
    43201 ataaatgtaa atccagttgg aaaaacaggt cctttagcaa aatttataac agaagaatat
    43261 tatgtatcag atgttaatcc taactgtata agtgttgtag atggggaaga tatgccacaa
    43321 aaatctcaag aaaataaaag cgaaaataag agttctatta gaaagcctga taattatgat
    43381 gaggttaagt caaaagcaca aggtgaatat gctaaaaaga acataatact ttctattggt
    43441 caaggggcag ggcaagtagt atcaaaattt tatgatgcag gtcgagacac tatccaaatg
    43501 gtaatgaata acgtaatacc atttatggca tttgtaagta tgcttatggg aattatttta
    43561 gcatctggac tgggagactg gattgcaaga gttatatctc cacttgcagg taatattggt
    43621 ggattattga ttatatcagt aatttgcaca cttccattct tatcaccaat actaggacct
    43681 ggtgcagtta tagctcaagt agtagggact cttgtaggta cacaaattgg tcttggtgca
    43741 ataccagcat atttagcact tccagcttta tttgcaataa atgggcaagc tggatgtgat
    43801 tttgtaccag taggattaag tttgggagaa gcagaaccag aaactgtaga gtatggtgtt
    43861 ccagctttat tttactctcg tttaataact ggtccaatag ctgttattat tgcatatgga
    43921 gttgcagtat ttgcattgag ataatattta atgtcaatat aaggcttgga attaattaat
    43981 aaaattgtaa atataaattt aagctagtca agattttttg ttaatgaaag actagctttt
    44041 atactattaa ttagctgtaa ttatcgagtt agattaatac taaattttcc tttattaaag
    44101 tgaaatttta tgtattaatt tggtttcaaa tttgtactag aatgaaatca aggaggagat
    44161 acaaaatgag tgtaactgag aaaattcctt ctattgaccg caagatattt aagatattga
    44221 ttatgtgtag taaaaaagag tttgtgagta ttaattcaat agcaaatgaa ttgaatgtca
    44281 caactagaag tgtaaggacg tatataaaac aattgaataa agatttaggt aatgatatag
    44341 cagtaatcaa atatataaag ggtcaaggct ataaactaga aataaaagat gagcagatac
    44401 tcaataagat tattgatatg aatagaaaaa atgtattttc attaaattca aaagaagata
    44461 gagtagagtt tatactaaat tacttgatag aactagatgg atttattaca cttgatagtt
    44521 tagctgatga gatgtgtgta ggaagaacta ctttagtaaa tgatttccag tatgtagaaa
    44581 aagttttggc ttcatataat ttaaacttga ttaaaaaaca gaatacaggt atgaaattaa
    44641 atggaaatga acttgatatt agactgttta tattaaatca gttgtataaa aactcaagga
    44701 aagatttcag taattcaaag tattttaaag gaatcaaaaa agaagaaatc ataaatttag
    44761 aagaaaaact attgacttta tttaaagaaa ataattttta tgttacagat gaaatgttaa
    44821 gagaagttat taactacata gtagttttag tctatagagt aaaagaagaa aagaaagtaa
    44881 aagactatga tgtaaagttt gatttactta aatcatatga tgaatacttt attgctaggg
    44941 aaataaaaca tataatatca ggaatgtttg aatgtacttt aaatgatgaa gaaattattt
    45001 atttaactat accattagtt agtggaaatg caccagcatc agaatgtgct ttaaatagta
    45061 gtagaattag taaaaacatt gatgagttga tggaaaatat atttaatcaa atatatgtcg
    45121 atatgggtat ttttataaat gaggatgaat taagagtagg tctaggatat catcttagtt
    45181 ttactttaaa caggctatta tttaatataa agttaaaaaa tgttttgtta gaagagatta
    45241 agcaaaatta tatacttcca tttaaattag cacaaatagc aggaaaagta atagagaaaa
    45301 agtataactt agaagtttca gaagatgaga ttggatatat agcaatccat ttcagtggat
    45361 atttagaaag aaatagcagt agattctatt caataaaaaa aatagcaatt atatgtagta
    45421 caggtctagg aacagcaaaa ctattgaaaa taagagtaga aaaactgata ggaaataatc
    45481 ctaaaattga tactctttca agttttaatt tgagaaatat taacttagat gaatacgata
    45541 tagtttttac aactattgat ttagacacat cgaatataaa tacaatagta ttaaaaataa
    45601 atacaatatt tgatgaaaac aaattaagag aacaattaaa aacagtattg tgcctaagag
    45661 aagggaatat tgacactact aattctacaa atttactaat aaataatttg ttagatgaag
    45721 ataaatttat gatattaaat gaaaagacta tacttgcttc attagaaaaa atgatggacc
    45781 atcttatgga tttgggatgt attgatgata agtttagaaa aaatatagtc aatagagaag
    45841 aaaaatctcc cacagtattt gataaaggtt tattatttcc tcactctgta aatgaaaaat
    45901 cagataagtt tttaatggca gttggaattt tagaagaacc aatagaatat gcaaatagaa
    45961 gtataaaaat aattcttatg acaatgtttc catgtgagaa taagatagac tcagacttat
    46021 tagtaaaaat ttatgaagaa gttttaaaga taggccaaga tgtaaaatta acgaataaga
    46081 taagtaaatg tagaactttt ttagaattta aaaaggtgtt acttaaaaac cttatataac
    46141 tattgtacag catttatagt tgttatacag atttaaaaat aatgtgattt ttggaaggtg
    46201 tataaatatg aaattattag taattttatt tgttgcttat attttaaata ctatatttat
    46261 aatgaagcag aataaattgt attttaaaac tttaaataga gctaaaaaaa agggtgatat
    46321 agtgtctaca ggcaaaaaga aaagctattt ttctaaagga agtatagcca ttatatcttc
    46381 agattcagat gggtttataa agtttggaga aattttaaaa gggcgtacag taatggctaa
    46441 atttaaagag attgaagaaa tagaaggatt agatatatat agtgcagagc aaaaatttaa
    46501 agatgaagag agtattgtac aagctataaa atttataaaa gaaaaaataa atgtttcttt
    46561 taattaactc aatataaata taaattatca ggaggaatgt catgaaatat gaagttaaga
    46621 taagtgggat aggcagttta gttaaagagt taatggatga aagcaattgt cttattatat
    46681 atgatgaaac cattaatgat gacgacttaa aggatatatc tgtaattcat tcgatagcaa
    46741 cattaaaaag tgatgttgaa ataggagata cacttactat agggaataga gattattgta
    46801 tagtttcagt tggtgatatt gctcagaaga ctcttagaga aattgggcat tgtacaatca
    46861 aatttgatgg aaaatgtgaa gtaaatttac caggagaaat acatgtggaa ataggaaatc
    46921 cagatataac tattggagat ttaataacta tttcttaatt tgaaaataaa attattataa
    46981 caggaggaat tggtatgtta gatttaaaca gaaaacttgc aaaattggaa gaggaaggta
    47041 aaaaaataaa agtagctctt ataggagctg ggcatatggg aaatggaatg gtaagccaaa
    47101 tggcaaatat gaaaggtatt gaagcttcta ttgttgtaga tataaatttg gagttggctc
    47161 ataaagcttt tactgatgca ggaatagatg aagaaattat agataatgta acaaatgaaa
    47221 aggatgcaga aataaaatta caagaaggaa aagtattgac atgtaatgac tttcttgtgg
    47281 cttgtaaaac taagtcaata gatgtagtta tagatgcaac tgggggtatt gcaattggtg
    47341 cagagatagc attaaactct attttaaata aaaaacatat agttatgtta aatgctgaaa
    47401 ctgattgtgt tgttggacca atacttaaaa aattagcaga tgatgctgga gtgatattta
    47461 caggttctgc aggagatgag ccaggtgcag taatggaatt atttgatttt gcagatgcaa
    47521 tggggtttga agttagagtg ataggtaaag gtaaaaataa taaattagat ttagattgta
    47581 atcctgatac agttagagaa gaagcagaaa gaaaaggagc ttcacctcat atgatagctt
    47641 cttttaaaga aggaactaaa acaatggtag aaatggcttt aatgtgtaat gcaactggat
    47701 ttgtgcctga tgtaagaggt ggtcatggta ttgaagctac agttaatgaa gtacctaaga
    47761 aatatgcatt aaaaagcgaa ggtggagtat tagataatta tggtgtagtt gattttgtaa
    47821 atggtatagc acctggagtt ttcgtagtag tagcccataa attaaaagca gttaatgatg
    47881 aactaaaata tttaagtatg ggtgatggac ctaattacat attatataga ccatatcatt
    47941 tatgtagttt agagactcct ttatcagcag ctatggcagt cttagaccat aaggctacaa
    48001 tagtacctaa agctggacta gtagcagaag ttatgactat agctaagaaa gatttgaaaa
    48061 aaggtgagtt tatggatggt tatggagcat atacatgcta tggtactata gaaaaatacg
    48121 atgtagcaaa ggctatgaat gctgtaccta ttggtttgat aagtaaaaag actaaagttg
    48181 taaaagatat taaaaaaggt gaagttataa cttacgatat ggtagagata gaaaaagata
    48241 ctactttata tcatttaaga caattgcaag aaaaaatatt tgggtaagat ttgatggtaa
    48301 tataaataga tattagtaaa aagggttgat ggttattttt aaatcaaccc ttttaaattt
    48361 ttatatattc ttgttaggat actttcttta aaagtatatt ataggttatt tgtagtcgta
    48421 aaaattattt tgaattagtt ctgcaatttt atattcacat tgaaacaata ttattaaaaa
    48481 ttttaatata aaatgaatta aaatgaccaa tta
//
LOCUS       DAELSY010000009        47570 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.009, whole genome shotgun sequence.
ACCESSION   DAELSY010000009 DAELSY010000000
VERSION     DAELSY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47570
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.009"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      115..200
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            362..883
                     /locus_tag="KPG09_000451"
     CDS             362..883
                     /locus_tag="KPG09_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0965080.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNDEGVTLTEAQSAKLLSDIGENKIYTSVTADNLGNPLTSNTKVDNKVLY
                     IVLIDNTVMDSTK"
     gene            1034..2710
                     /locus_tag="KPG09_000452"
     CDS             1034..2710
                     /locus_tag="KPG09_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG0965081.1"
                     /translation="MAKKVRIGDKLVEKGYITEDQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            2722..3930
                     /locus_tag="KPG09_000453"
     CDS             2722..3930
                     /locus_tag="KPG09_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG0965082.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            3945..5657
                     /locus_tag="KPG09_000454"
     CDS             3945..5657
                     /locus_tag="KPG09_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBG0965083.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETHDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            5650..6558
                     /gene="pilO"
                     /locus_tag="KPG09_000455"
     CDS             5650..6558
                     /gene="pilO"
                     /locus_tag="KPG09_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBG0965084.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            6585..7151
                     /locus_tag="KPG09_000456"
     CDS             6585..7151
                     /locus_tag="KPG09_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0965085.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            7188..7715
                     /locus_tag="KPG09_000457"
     CDS             7188..7715
                     /locus_tag="KPG09_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0965086.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            7736..9274
                     /locus_tag="KPG09_000458"
     CDS             7736..9274
                     /locus_tag="KPG09_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965087.1"
                     /translation="MRKWNKFKSERGAALVLVLIVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEKNIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            9374..10456
                     /locus_tag="KPG09_000459"
     CDS             9374..10456
                     /locus_tag="KPG09_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBG0965088.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESIFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            10437..11219
                     /locus_tag="KPG09_000460"
     CDS             10437..11219
                     /locus_tag="KPG09_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG0965089.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGILFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILIFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            11321..11971
                     /locus_tag="KPG09_000461"
     CDS             11321..11971
                     /locus_tag="KPG09_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG0965090.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     AQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            11985..12545
                     /locus_tag="KPG09_000462"
     CDS             11985..12545
                     /locus_tag="KPG09_000462"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBG0965091.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            12562..15948
                     /gene="mfd"
                     /locus_tag="KPG09_000463"
     CDS             12562..15948
                     /gene="mfd"
                     /locus_tag="KPG09_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBG0965092.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGAENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            15979..16974
                     /locus_tag="KPG09_000464"
     CDS             15979..16974
                     /locus_tag="KPG09_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0965093.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            17098..17637
                     /gene="spoVT"
                     /locus_tag="KPG09_000465"
     CDS             17098..17637
                     /gene="spoVT"
                     /locus_tag="KPG09_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBG0965094.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            17757..19370
                     /locus_tag="KPG09_000466"
     CDS             17757..19370
                     /locus_tag="KPG09_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG0965095.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            19477..20925
                     /gene="mazG"
                     /locus_tag="KPG09_000467"
     CDS             19477..20925
                     /gene="mazG"
                     /locus_tag="KPG09_000467"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBG0965096.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            21014..21292
                     /locus_tag="KPG09_000468"
     CDS             21014..21292
                     /locus_tag="KPG09_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBG0965097.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            21364..21606
                     /locus_tag="KPG09_000469"
     CDS             21364..21606
                     /locus_tag="KPG09_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBG0965098.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            21668..21922
                     /locus_tag="KPG09_000470"
     CDS             21668..21922
                     /locus_tag="KPG09_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBG0965099.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            21923..22456
                     /locus_tag="KPG09_000471"
     CDS             21923..22456
                     /locus_tag="KPG09_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965100.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            22462..22746
                     /locus_tag="KPG09_000472"
     CDS             22462..22746
                     /locus_tag="KPG09_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBG0965101.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            22859..23779
                     /locus_tag="KPG09_000473"
     CDS             22859..23779
                     /locus_tag="KPG09_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBG0965102.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYDEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            23869..26241
                     /gene="spoIIE"
                     /locus_tag="KPG09_000474"
     CDS             23869..26241
                     /gene="spoIIE"
                     /locus_tag="KPG09_000474"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBG0965103.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNNEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            26592..28484
                     /gene="pepF"
                     /locus_tag="KPG09_000475"
     CDS             26592..28484
                     /gene="pepF"
                     /locus_tag="KPG09_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG0965104.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAIAVMNNN
                     DTEDDAEKKQEVDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMSDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESVDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            28598..29563
                     /gene="galU"
                     /locus_tag="KPG09_000476"
     CDS             28598..29563
                     /gene="galU"
                     /locus_tag="KPG09_000476"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG0965105.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            30063..31667
                     /gene="rho"
                     /locus_tag="KPG09_000477"
     CDS             30063..31667
                     /gene="rho"
                     /locus_tag="KPG09_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBG0965106.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQENRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            31820..32020
                     /gene="rpmE"
                     /locus_tag="KPG09_000478"
     CDS             31820..32020
                     /gene="rpmE"
                     /locus_tag="KPG09_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBG0965107.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            32165..33058
                     /locus_tag="KPG09_000479"
     CDS             32165..33058
                     /locus_tag="KPG09_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBG0965108.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            33076..33924
                     /gene="prmC"
                     /locus_tag="KPG09_000480"
     CDS             33076..33924
                     /gene="prmC"
                     /locus_tag="KPG09_000480"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBG0965109.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            33968..35032
                     /gene="prfA"
                     /locus_tag="KPG09_000481"
     CDS             33968..35032
                     /gene="prfA"
                     /locus_tag="KPG09_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBG0965110.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            35161..35841
                     /locus_tag="KPG09_000482"
     CDS             35161..35841
                     /locus_tag="KPG09_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBG0965111.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            35870..36910
                     /locus_tag="KPG09_000483"
     CDS             35870..36910
                     /locus_tag="KPG09_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBG0965112.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            36927..37376
                     /locus_tag="KPG09_000484"
     CDS             36927..37376
                     /locus_tag="KPG09_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBG0965113.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            37393..37845
                     /gene="rpiB"
                     /locus_tag="KPG09_000485"
     CDS             37393..37845
                     /gene="rpiB"
                     /locus_tag="KPG09_000485"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG0965114.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            37881..38510
                     /gene="upp"
                     /locus_tag="KPG09_000486"
     CDS             37881..38510
                     /gene="upp"
                     /locus_tag="KPG09_000486"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBG0965115.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            38810..39625
                     /locus_tag="KPG09_000487"
     CDS             38810..39625
                     /locus_tag="KPG09_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBG0965116.1"
                     /translation="MKNCIAVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            39851..40288
                     /locus_tag="KPG09_000488"
     CDS             39851..40288
                     /locus_tag="KPG09_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBG0965117.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            40473..40700
                     /locus_tag="KPG09_000489"
     CDS             40473..40700
                     /locus_tag="KPG09_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBG0965118.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            40705..41091
                     /locus_tag="KPG09_000490"
     CDS             40705..41091
                     /locus_tag="KPG09_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBG0965119.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAIRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            41094..41798
                     /gene="atpB"
                     /locus_tag="KPG09_000491"
     CDS             41094..41798
                     /gene="atpB"
                     /locus_tag="KPG09_000491"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBG0965120.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            41867..42127
                     /gene="atpE"
                     /locus_tag="KPG09_000492"
     CDS             41867..42127
                     /gene="atpE"
                     /locus_tag="KPG09_000492"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBG0965121.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            42250..42747
                     /gene="atpF"
                     /locus_tag="KPG09_000493"
     CDS             42250..42747
                     /gene="atpF"
                     /locus_tag="KPG09_000493"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBG0965122.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            42744..43289
                     /locus_tag="KPG09_000494"
     CDS             42744..43289
                     /locus_tag="KPG09_000494"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBG0965123.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            43306..44808
                     /locus_tag="KPG09_000495"
     CDS             43306..44808
                     /locus_tag="KPG09_000495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBG0965124.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            44836..45714
                     /gene="atpG"
                     /locus_tag="KPG09_000496"
     CDS             44836..45714
                     /gene="atpG"
                     /locus_tag="KPG09_000496"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBG0965125.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            45727..47121
                     /gene="atpD"
                     /locus_tag="KPG09_000497"
     CDS             45727..47121
                     /gene="atpD"
                     /locus_tag="KPG09_000497"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBG0965126.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            47124..47384
                     /gene="atpC"
                     /locus_tag="KPG09_000498"
     CDS             47124..47384
                     /gene="atpC"
                     /locus_tag="KPG09_000498"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBG0965127.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
BASE COUNT        19244 a         5076 c         8886 g        14364 t
ORIGIN      
        1 atatgtaaaa agttggtata tataaatagt ataaacagtg ttgctaaaat atggtagata
       61 ttatataata taagtggata tgtatataca ttatgagtta tataattttg aatttaataa
      121 aatagaaacg ttgatttatg ttctgtaatg tgggcacctt agagcatatt gagttagtgg
      181 tgcaaccggc tatgaaattg tatttattta tagatactat cattttcata gcttttttta
      241 tttaaaaaat ggttatgtgt tagaaataaa tttggttagt acgtttaaat aattttactt
      301 agatttttaa ccagatatat tataagtaag tattaattaa aaaatttaag ggggaataaa
      361 aatgaagtta aaaaagaata aaaaaggttt cactttagtg gaattattgg tagtaattgc
      421 aattataggt atattagcag tagtggcagt tccagcttta tttagtaata taaacaaggc
      481 taaggtagca agtgttgagt ctgattatag ttcagttaag agtgcagcat tatcttatta
      541 ttcagatact aataagatac cagttacacc agatggtcaa actggtttaa gtgttttaga
      601 gacttatatg gagtctctgc ctgataaagc tgatataggt ggaaaatata aattgattaa
      661 agttggtaat aaattggtat tacagatagg tacaaatgat gaaggagtta cattaacaga
      721 agcacaatca gcaaaattat tgagtgatat aggtgaaaat aaaatatata caagtgttac
      781 agcagataat ttgggaaatc cattaacaag taatacaaaa gtagataata aagttctata
      841 tatagtactt atagataata ctgtgatgga ctcaacaaaa tagttaaaac tatattttta
      901 taaaaatatt gtaaatttaa tgaaaaatat aatataaaag acaggaagaa aatcttcctg
      961 tttttttata ctattttctt taatgttcaa ttatgaatga tgatataatt gagatagtaa
     1021 aggagtgaaa cctgtggcta aaaaagttag aataggagac aaactggtcg aaaaaggata
     1081 tataacagaa gaccagctta aatgggcact atcagaacaa aaaaacagtg gaaaaagact
     1141 aggagaattt ctagtacaag aagggcttat agatagtaac ttactaataa gtgtattaaa
     1201 agaactcttg gatatagaaa gtatattttt agaagggaca gaaatagaca ctttagctac
     1261 aaagatggtt ccagaaaata tatgcaaaag atatactgta tttccattta aaattgatgg
     1321 aaataaaata tgtctagcca tgtcagaccc acaagacaga gaagcagttc aagatgtaag
     1381 gcgtatgtct ggtaaagacg tagagatatt tatatcaagt acagaagata taaacaaagc
     1441 aataggtcat gcttatgcac attcagaaat aaataaggca atgactgagt acaacaaaaa
     1501 tagaactggt ggagtaagag aaacagtaat acttgaagaa gatgtaaatg ctgctccaat
     1561 agtacgatta gtaaataaca tactagaaaa tgctgttaga atggaagcaa gtgatataca
     1621 tatagaacaa agtgaaaatt acatgcgtgt taggtttagg atagatggaa tgttaagaga
     1681 atatatgagg atgaactcag caccatataa ggcagtaatc agccgtatca agataatgtc
     1741 agatataaat atatctgaaa agagaatacc acaggatggt agaatctatt taaaagtaga
     1801 taataaaccc atagatttta gggtttctac aatgccaact aacagggatg aaaagatagc
     1861 tatgagggtt cttgataaga gtaattttat ggttagtaaa gaagtactag gaatagacga
     1921 acatggctct aagatatatg atgaattgat aaacactcct tatggtctta tattggttgt
     1981 tggtcctact ggaagtggga aaaccactac attgtattca atgttaaatc aactaaatac
     2041 agaaaataga aatctattaa caatagaaga ccctatagag tatgaattac ctggtgtaaa
     2101 tcaatctcaa ataaatgaaa aggcagggct tactttcgcc agcggactta gagcttttat
     2161 gcgtcaagac ccagatataa taatggttgg agagataaga gatactgaga cagcagaaat
     2221 agcaattaga gcttctctta caggacattt agttttaagt acattacatg caaatacagc
     2281 agttggagca atttcacgtc ttttagatat ggatgtggaa agttttttaa taacttcatc
     2341 tgttttaggt gtaatatctc aaagacttac acgaaaaata tgtgagcatt gtaaggtttc
     2401 atatgaagca gatattggag aaaaaaaggc tcttggtatt gatgtaaatg aatcagtaac
     2461 tatttataga ggaaaaggtt gtgaaaggtg taataatact ggatataaag gacgtctagg
     2521 tatatttgaa atgcttgaaa ttactccaga aataaaagag ttaatagatt catctgcaaa
     2581 tcaaagagaa attttaaaga tggctagaaa acaaggcatg gttagcttaa aagaggatat
     2641 agttaaaaag gttttaaatg gaaagacaac agtagaagaa atgattagaa taattttgat
     2701 gactgattaa aggatgatat tatgaaaagt tttaaataca cagtaataaa agaggacgga
     2761 aaaaagcaaa ctgatgtgat tgaagccaac gattttaatg aagctagaaa tcttcttaga
     2821 aagagaaatc ttagagttat tgaaatcaaa gaaagcaaaa ataaaaatat aagattatct
     2881 agtagaaaga gaaaaaaaga cttgggagct gaccagataa gccacttttg tagacagttt
     2941 gctattatag tatcttctgg tataaatagt atttcaggat tagaaacatt agctagaagg
     3001 tcaactaaca ttacattaag agaagaaatt aatcgtatag tggcagaaat aaaagtaggt
     3061 tctactattg cagattcaat gctttcatca aaatcaaagt ttccaaagtt acttggtgct
     3121 atggtagcta caggtgaagc tacaggtaaa ttagatgaag tattaaaaag tatggcttca
     3181 ttttatgcaa gtgaacacag ggttaaacaa aagcttagaa atgctgcaac atatccatta
     3241 atagttttga tttcttcatt tataatgatt tttatattta caacatttat ggtgcctaag
     3301 atgattaatt caattacaac agtaggagca actcttccac ttataacaag aattgttatg
     3361 gggtttggta tgtttatgaa gaagttttgg ataatagtac ttttagttat tatattattt
     3421 atctatcagt ttaaaaaata tttaaaaacg tcagttggaa gggctcataa agatagagtt
     3481 ataaacaaga tacctgtttt aggaaaagga ataaattgta tggtagctac acgattttca
     3541 agagcattgt atctatttgt atcaacagga tacccattag ttcaagggct tgattatatt
     3601 atagatagtg taaataacac catggcagag aagattttag catcagctaa agatggaata
     3661 acaagaggag aaggcttagc agaaaattta gagagatata cgtattttga ctctgtgttg
     3721 gtacagatga tagctatagg ggagcagaca ggggaattag agaatataag tagacagatg
     3781 gcagaatttt atgagtatga atccgagatt tatttaaata ggatggcctc aatgatagag
     3841 cctgttttaa taattgcagt tggtattata gttgcgatac tggttgtatc aatatttatg
     3901 ccaatgctta gtatatatga tgccatgtag gagggaaagg acaaatgaat aaggtttatg
     3961 tatcatatta tggcgattct atttccatag ttgagggcag atataaacac gataaggata
     4021 agttttatgt aaaaaatttt aatgtgtttt caatagagga tattgcacaa gatttttcta
     4081 aggaaaagta tgctctattg agatacgcat tagaaaatgt taagttaaaa tcaagacatg
     4141 tagttttttg tttaaataca agagatgtaa ttttaaaacc ccaaaaactt cctaatatgg
     4201 acaaaaagga tttagatgga tttatgaagc tagagatgga cgaaatgatg gctttggagt
     4261 cagatgaata tgtattttcg tatgaagtat ctagtgaaac acatgataat tcagatggcg
     4321 atgaatcaag cttaaatctt atcattggag ctatacaaaa ggaagaggta aatgcaattg
     4381 tagagataat gcatgaattt gatttagtat tagatagaat agatacatta tcaacagctt
     4441 acttaagaat gcttaaaaat ttagactaca atgatataat ggtaaccaat ataagtgaca
     4501 atagtacaat tataaatata tataagaaag atactttatt tattagtgat aatataccta
     4561 taaagataac tgctaaagat atggacatgc aattattaaa tgtagtagaa gaatcaaaag
     4621 gtcttatgaa ttactattcg tcaagaaact ttggtaaggt tacggatact atattaatat
     4681 taggaaaaag atattccaat aaattaattt atgaaacttt tagctctgca ttttcaagct
     4741 atgtttctca aggccttact gagataatgg atatatctga tatgataact ggagatatag
     4801 atgaagatga tataaattca attgtagagc ttttaggaag tatgatagaa cctaaaggga
     4861 aaagagattt tgaaaatata aactttttac caatggatat acttagaaaa cagataaaag
     4921 ctaaacaaat gaaaaacata ctgaaaattg ctccaatagt tattttgatt cttgctatac
     4981 cttatttatg tttaattttt tcaaataatt tagcaaataa ggaacttgaa aatgttaatg
     5041 gtgaaataag aaatattgaa tctgcatatt atcagattgg aaatatagaa aaaatgatac
     5101 agagtaaaag cgaagaaata aaggtatatg atatgttaat tgggaaaaag gtaaagtggg
     5161 aaaatttatt ggatgatata gataaaaata ctccaagtaa agttgaacta acaagtttat
     5221 caacaactta tcaaaatctt ggaactaata ataaagctga taattcaaaa agtaataaga
     5281 ctacaacaga taaatcaagt aacaatgaaa caaaatcaag tacgaaaagt gatagtaatg
     5341 gagataaaac agataatgat ttaaatgaag ataagactac aaatgtgtca gaagcattaa
     5401 ctggaaatag tgagactaat agtaataaca cggataaaga tgataaaact ccattgtatg
     5461 ataagatacc aaatgttata aatatagaag gaaatgctaa agattcctct tatgtaggtc
     5521 aatttttata taaattaaag ggattatact atttcaagga tgttaaatta catagcataa
     5581 aaagtaatgc agaagattct aactatacat ttagtatgac attatacctg aaagaaggtg
     5641 tattaactaa tgaataaaga tatatttaaa aaagatataa aagatatatt taaaatggaa
     5701 gttagtctaa aagggattct tataaaggtt ataattttat tagctatttt atcatatggt
     5761 tattttggac tttaccctca atatcagaaa tatattgcta caaaaaattc tgtaaaacag
     5821 gctactgaaa aattaaatat atataaagag aaaactgcac ttatgccaaa atatgaaaaa
     5881 caacttgaag aagttgaaag tgaattaaaa gaaaaaagca aaaatctttt atatgatatg
     5941 caagatggtc tttttttaat aggtttagat aaaaaaatga aggcttataa tattgactta
     6001 actagttatg acgttggaaa tgttaaagaa tacgaacatt tttatggtat atcaacaaca
     6061 ataagtgtta gaggtgatta tagaaatgtc aaaaaattaa tggcttattt agaagaacaa
     6121 aaaaatgtta ctcaaatact agattataca atgacttcat atatagaacc agaaaaagaa
     6181 gaggaagaaa caacaaacaa taaggattta attaaaaatg tgaacataaa taaagataca
     6241 aaaatatact gggtggaagg cgaaaataag tggcatttat ataaggattg tccattgata
     6301 aaagaaggag agattctaaa aaatgacaaa cttttagata ggtctactaa ttatctatgt
     6361 aatacttgtg tacttagagc tgcaacagaa aaagaagcaa aaaaagaagt cgtagctcca
     6421 aaggcagaag gtgtagtaga tgctacattt aaatttataa tgtacactaa agagaatact
     6481 acaatgatac ttgatacaga taatccatct tcatggagac caggaaaata taatccattt
     6541 aagtctagtg ctaaataaag ctaagtaagc tttaaagata ggtgatgagt ttgtacaaaa
     6601 ataatgaaaa aggtcttact ttattagaag taataatagc tgtttttata ctgacaatag
     6661 ttttaagtat ttcttataaa gtgttcaatg gtataacatc agcagtaaaa aagcaacaga
     6721 ttattacaga tgctcaagta aatattaatc taattaataa gtatctaaat agagatttgg
     6781 aaaactgtaa agagctaact aaaactggtt caggtaataa ttatgaatac aatatagaga
     6841 tgccagataa tgttgtaaaa tatgaagttt ctatagaaac taaaaaaaat acagaggtat
     6901 attctgtaac gagaatccaa aacaatacaa ttgatacaga aaatgaagtt agagaagaga
     6961 taatctataa tcaaccacta gttcaaaaca ataaagaaat gaaggaaaca ccatttaaaa
     7021 tagaaaagca aactggtaaa tctatttata ctgtaagtat atactataat gaatcagtgc
     7081 aagaaagtca taaaaatagc aatttaaata acaaaactta tacatttgat gttatgtcaa
     7141 gaatagggta ataagaattt attaaaaaat aagtaggttg ataaaatatg agtaaaaaga
     7201 atagtgaaag aggattttcc ttaattgaag tgttagtagc tatggctatt atgggaattg
     7261 tactatttgc attttttaat attatcaata ctaataacaa agcaaacact aaaaatgaca
     7321 cagatataac ttccttaaac tatgtccaaa gtgaaataga aaatctaaga gaaaagataa
     7381 aaagtggaga atttgatttt gatagtttag ataaactaga agatggaact gtcgtatatg
     7441 aaaaattaat agataaatca aaaaaagttg tatatgataa agttcttagt gaaggtgacg
     7501 tgagcttata tgatactcca tatgaaaaga ttacaacaat aaaagatgaa gatggtaatt
     7561 taatcgacaa aggaaatatt acaaataaga taaagactat tgtagaagat aaaagtggtc
     7621 agatatataa aatagctgta actgggaaaa gcatgaatga ctattcaagt aaaaaagagg
     7681 ttaaaattgt aactgaaata tttaaagata aataatttta cttgggggtg agattttgag
     7741 gaagtggaat aaatttaaaa gtgaaagagg ggcagcttta gttttagttt tgattgtagt
     7801 agcattgctt tctattgttg gattgatatt ttcaaatcag atagctaata gaataaaatc
     7861 tacaaagact actaatgaag gtatacaagc aaagtattta gcagagacat gtgtggaaaa
     7921 tagtattgat aaagcatatg aaaaattgta tgatgagctt gagaaaatgg ataatgagtt
     7981 taaaagtgaa aatcaagaaa aatccatatc taggtcaaaa ttaagaaata tatcagatga
     8041 agattttaat aatcaagatg agaaaaatat tgaagcagaa agacttggat atatgaataa
     8101 tataaatttc tatttaaata aagcttcttc agacttggaa aaagctagta tggaattgaa
     8161 aaagctatat gatttagata tgttggacta tagagacatt gaatatgtag atgcaaatat
     8221 tatttcacat agagattcta tcttagaaat ttgcaagaat tatacttctg gagatattag
     8281 taaaataaat gaatatattc tgaaagaaga tatagactca actacattga ttgaggcaaa
     8341 gttagtgaat aatgatattc ttttgaaaat gtttttagaa gaaaataaaa ttgaaaatga
     8401 acatttaaat tcagcctttt cacatactta taaggcatta gataacattt ctttggcgat
     8461 gcaaaatatg attgagtata gacatacgtt tcatatagat gagccaaaag tagaagtatc
     8521 taatgggatt ccagattctc aacaatatta tgaactaata caaaatccta taataaatag
     8581 tatggagtat atctggaata gtaaatggga tacattagaa aacctattgg aaattttacc
     8641 aaatcaaact caaggattta attctttaag agttcatttg agaaataatg taagaaaatt
     8701 tgagaagtta agcgataata tatctagtgg taaaaaaaat acagcaaaaa attttttaaa
     8761 gtataaagag ttattgtatg aaatatcaga ccaatgtaat caactaaaaa gtatgtctta
     8821 tgagaaaata cctgttaaat atgataatat ggctttgata acaacttttg actatataca
     8881 aaatgagctt ttagcagaga taaaatgcag attaaaagaa ttaaaaccac aagaaattga
     8941 taaaacagaa ggtattacta taaaaatacc tttttataag gctgactacg acatgacaaa
     9001 agaaggatgg cctaaattaa aagaaaatgg ttcaggggcg gagttaagct taatggtaac
     9061 tggtgataaa gatggaataa aagaagtaga agttacagat ggtaaaaaaa atattattgg
     9121 tttaggtgtt gaagaaaatt caaattcaaa gtataaggtt gacgccattg taaactttaa
     9181 tctaaatata gatacaaatg tagttggaaa ttatgatata aaagataaaa ttttgattaa
     9241 tcatgatata agttcataca aaaaagtaaa ctaaatataa gaagcaactt agtataaata
     9301 aataataaaa taattttatt atttactact aaaaaaataa gtaaactaat agaagatatg
     9361 gagagtaaat attatgtata tttatgattt attagagcaa ggtataagac taaacgcatc
     9421 agatatacac ataacagttg gaacaaatcc tgtagctaga gtaaaaggtg gatttgtaaa
     9481 actaagtgaa caaatattga catcagaagt aacaatgcaa atggcaaaag acatagcggg
     9541 ggaatctatt tttaaagtaa tagaagaaca tggagaagct gatttttcag cttctctaaa
     9601 aacaggagaa agatttaggg taaatgcata taggcaaaaa ggaaattacg caatagcaat
     9661 aaggactata acagcagaaa ttccaacatt tgaaaaatta ggccttccag agagtataaa
     9721 atcttttact gaaaagcata aaggattagt tttagttaca ggtccaacag gaagtggtaa
     9781 aagtacaaca cttgcaagta tgataaatat aataaatgaa aaacaacaaa aacatataat
     9841 tacacttgaa gacccgatag aatatgtaca tcatcataaa caaagtttag taaatcaaag
     9901 agaagtagga actgatacag aaagttttca ttcagccctt agagctattt tgcgtcagga
     9961 cccagatgta atactaatag gagagatgag agaccctgaa actgtttcaa tagcattaac
    10021 agcagcagaa acaggtcatt tagtattttc aacactacat acagtagggg cagctaagac
    10081 aattgataga atagtagaca tgtttcaacc atcccaacag caacaaataa aaacacagtt
    10141 atctacagtt tgtgaaggtg tggtatcaca gcagttactt cctacagcag atgggaaagg
    10201 cagaatagca gcaattgaat tgatgtttgc tactccagca ataaaaaact tgataaggga
    10261 aggcaaaaca tatcaaattc caaacatgat acaaactgga gttaaagtag gtatgaaaac
    10321 tatggaccaa gatttaatgg aactttataa aaatggaaag attacaaaag atatggcact
    10381 tagtaggtgt actgaccaag agtttatgac aagaatgata ggtggagtaa actacaatgg
    10441 atattataat cgttaatcta tatgtattta tagtgggtat agtgtttgga agtttcttta
    10501 acgtctgtat ttttaggatt ccagaaggta tatctatttc tagacctcct tcccattgta
    10561 tgagttgtaa tactagacta aagccaatag acttaatacc agtgctaagc tatttgatga
    10621 gtggcagaaa gtgtagatat tgttatgaga aaatttctag tagatatgct atagtggaat
    10681 taattacagg aattttattt ttaacagtat ttatgttata tggaatatct acatcaacta
    10741 tttattattt ggttcttgtg tctttactga ttataattac atttatagat attgaccact
    10801 ttataatacc agataaaata ttgatttttg gagctatatt ttctatgatt tttaaccttt
    10861 tatttaaaga aattccagtt aaggattcaa ttctaggtgt ttttatttgt ggaggaagtg
    10921 tctggattat agttcttttg attgaatttg taataaaaaa agagtgtatg ggtggaggag
    10981 atatcaaact atttgcaatg ctaggccttt acatgggtgt taaaaatggt cttttgacag
    11041 ccttactaag cgtttatgta ggagcagtct atggaatctg tgtaataatt ataagtagaa
    11101 taaaaggaaa ggaatataat tctgttatac catatggacc ttttatatct attggagcat
    11161 taataacaat cttatgtgga aagcaattac ttgaacttta tttttctatg gttatataga
    11221 tgatagtcaa gttaaataaa gttttaattt aaaaaaatga attaaagtat ttaataagga
    11281 caatattatt attaacccta ttactaaagt gctacaaact atggagtata taataataat
    11341 ttccttattt tttgcacatt tgataaataa atctctatgt gttttttgtg atagagaaaa
    11401 gaaaaattta tataatacgt tttttattta cataataact gtagttttat ctgtattaat
    11461 atatctaaaa tttgggatta gtgtagaatg tgtacaatat atggttttaa ttccattttt
    11521 aataattata tccattgtag attactacac aatatatgta tatgatataa caatagttag
    11581 tggtataata gttcaaggta taattttttt ggcctttttc aatatagctc aatttgatta
    11641 tataaatcat atatttggac tattgatagg ctttattata ccttatatta ttgctaaact
    11701 aacaaaagga ttaggctatg gagatgtagg tctctatagc ttatgttgct ttgcattggg
    11761 aaataattat gggttttatg tgatagtttt atcatttatt ttagcgtttt tgttttttat
    11821 tattttatac ataactaaaa tatttaaaga tgtagatata agaaagcaaa ttccttttac
    11881 accttttatt tccttgtcaa caattttaat aatatttaca gagtataata tatttaattt
    11941 ttattataat ttgttataca aatttatata acaggaggta agaaatgtac gtagtagtag
    12001 ggttaggtaa tccaggaaag aagtatgaga agactagaca taacgttgga tttgatgtta
    12061 tagatattct tgctaaagaa tacaatatta gtgttactaa aataaagcat aaagctttga
    12121 ttggtgaagg gagagtaggt actgaaaaag tattattagt taaaccacaa acttatatga
    12181 atttaagtgg agaaacatta atagatatat ataaatatta taaggttgat ttaagtaata
    12241 tagtagtagt atatgatgat atagatttag aagttggaaa gatacgtata agaaaaaaag
    12301 gaagtggagg aactcataat ggcatgaaat ctataactaa gtgcctaggt tcaaatgact
    12361 ttccaagagt gagagtaggt gtatctaaac cagaagctgg tcaagactta gcagactttg
    12421 tactttctag atttagaaaa gaagaatctg ataacataaa tgaagcatta gaaaaagcgg
    12481 cagatgctat tgactcaata ataagagaaa atatagatat gtctatgaac aaatataatg
    12541 gatagtgaga gggagattta aatgaatgat gtttttttat atcctttgca aaattcaaaa
    12601 gagtataaag atataataaa ctgtataaaa aatacaaaag gctcattatt ggttaatgga
    12661 cttttaccag tacaaaaacc tcatatatcg tattctatat ttaatgattt atcacgacaa
    12721 atgattttta taacgagtag tgatttagaa gcaaagaaag tatacgaaga tttaagtttt
    12781 tatatggaag ataaagttga atatttaggt tttcaagata tttattttta tcacttggat
    12841 gcaaaggaca gaaacgaaga agcaaagaaa ttaaaagtat tgttaaaact agcaaataaa
    12901 gaaaaaatca ttttagtaac ttctattgag gcagttttaa gaaaatacat accaaaacaa
    12961 gttttgctcg atagtgtatc tcattataag gtgggagata gcttagactt agaaaagtta
    13021 actgagaaat tagtttcatt gggatatgaa agagtttcaa aaatagaggg gtttggtcaa
    13081 tttagtataa gaggtggaat tattgatgta ttttcacttg aatatacaaa tccaattcgt
    13141 atggaattat ttgatgatga gatagattcc atacgaactt ttgacgtata ttcacaaaaa
    13201 tcaattgata aattgcaaca attctctata actccatcta gagaatttat ttatcccgaa
    13261 aaaactacag atgcattagt taaattaaaa aaagaaacaa ctaaaaatac tgatgaaggt
    13321 acatttcaaa atatagatta tatatcaagt aagacttatt ttgaaggtgc agagaactat
    13381 atagattata tttatccaga agaaaacaaa agtatattta cttatttagc agatgatgca
    13441 atagtattta ttaatgatat aacgagactt aaagaaagat gcgaaaatta tataaatgag
    13501 tttagagaaa attataaatt aaatcttgaa agaggtctgg ctattaaaaa ccaaggtaaa
    13561 ttgttatatc actatacaga tttagagtac ttagtaaaag acaagtctgt ggtattaaac
    13621 tcgctattgc caaagtctat aaataacttt tctataaaat ctataataaa ttttgaaagt
    13681 agagaagttc ctacatttaa tggaaaggta gatttgcttg tagaagaatt aaatcggctg
    13741 aaatataatg gatacaaaat aattttagca acaaatacct tagaaagagc aaataaatta
    13801 ggaaaagatt tattggacaa aggcttagaa acgaccattt ctaaagatag agatattgaa
    13861 attaaatctt cacaagttat aattgtacct gcacacataa acagtggttt tcagtataaa
    13921 tctataaagt ttgttgttat aacagataac gaaatgatag gtgtttataa aagagcgtct
    13981 aagactagca ataaaaaagt taaaaaagga aagaaaatag aatccttttt agatttaagt
    14041 gttggagact atgtagttca tgaaaatagt ggtgtaggta gatacacagg gattgaacaa
    14101 attacagtca atgctattaa aaaagattat atgaaaatag tatatcaagg tggagataat
    14161 ctatatgtgc caatagacca gatggacaaa gtacaaaaat atattggtgc agaagtagaa
    14221 aaagttaagt taaataagct tggaacaaat gagtggacga aagcaaaagc aaaagttaaa
    14281 aaagaaattg aagatatgac aaaagacttg attgagcttt atgcaaaacg tgaaaaaata
    14341 caagggtata agttttcaaa agatacacct tggcaggcag agtttgaatc gttgtttcca
    14401 tatcaagaaa cagaagacca attaaaggct atagaagaaa ctaaaaaaga tatggaatca
    14461 agtaaggtaa tggataggct agtatgtgga gatgttggtt atggaaaaac agaggtagct
    14521 attagaagta tttttaaagc ttgtatggac caaaaacaag ttgctgtatt ggttccaaca
    14581 acaatattgg cccaacaaca ttataataca ttcaaagaga ggtttgaaaa ttatcctttg
    14641 agagtagaag ttttaagtag gtttaaaaca ccaaaacaac aaaaacagat tattgaagat
    14701 gctaaaaaag gattagtaga tattttaatt ggaacacata gaataatatc caaagatata
    14761 aatttaccaa acttaggtct tgtagtcatt gatgaagaac aaagatttgg agtaaaacat
    14821 aaagaagctt taaagaaaat taaatctaca gtagatgtac ttacactttc agcaactcct
    14881 ataccaagaa cattacatat gtctttaagt ggaataagag acatgagtgt tatagaagaa
    14941 cctccacaag aaagacatcc tgtaataaca tatgttacag agagtaagga aagcgtaatt
    15001 caagatgaga tagagagaga attaagtaga ggtggacagg tattttttgt atacaataga
    15061 gttgaacaca ttgaagaaat ggcaagtatg attcaaaaac tcgtaccaga tgctagagtg
    15121 gctgttgcac atggaagaat gacttctaaa tcattagaaa atataatcct aggattttta
    15181 aataaagatt atgatgtact agtctgtact acaataattg aaactggtat ggacatatca
    15241 aatgcaaata caatgattat atatgatgca gataaaatgg gattggctca actttaccaa
    15301 ttgagaggta gggttggaag aagttcaaga caaggatatg catatctttt gtatgaaaaa
    15361 gataaaactt taagtgagat agcggagaag agattaaaag ctatcagaga atttacagag
    15421 tttggttctg gttttaaaat agcaatgaga gacctcgaaa taagaggagc aggaaatatt
    15481 ttaggttctc aacaacatgg tcatatggca gttataggtt atgatttgta cgttaaaatg
    15541 cttaatgatg caattaaaaa agtcaaaggt gaacctattg tggaggaaat tgatgttgaa
    15601 atagatttaa gtgtaaatgc atacattcca gataattata tcaaagatga gttgattaaa
    15661 attgaaatgt ataagaaaat agcttctatt gaaaacaaag aagacatgtt ggatatacaa
    15721 gaagaacttg aggatagatt ctctgatata cctaaaccag tccagacact tcttacaata
    15781 gcttatataa aatcgttatg taagatatta aaaatagaaa agataagaca attaaaagat
    15841 gaaatattat tagttccaat aacaaagtat agaactaaac agaaaatagg ttataatata
    15901 gttacagaat tagaagaatt attagaaaag atgtgcaagg taaaataact tgtaaattta
    15961 agaagggatg ttttaaattt gaaaaaagta ataacattgg taatagctat gattttggta
    16021 gtatctgtga ctgcttgcag ttctagtaaa ggagaaactg tggcaacagt tgaaggtaca
    16081 aagatatcat cagatgaatt taaaaaaact atagctcttt ataaagattc tatggaacaa
    16141 acttatggaa aagatatatg ggataaggaa gttgaaaagg gtgtaaaata caaggacaag
    16201 tttaaagatt taattcttga ccaacttata actacagaag taatatattc tcaagctaaa
    16261 aaagataact tacttcctaa aaaagaggat gttgaaaaaa gttttaaaga attaaaagac
    16321 gctatgggta aagatgaaaa atataaagaa caacttaaga aattaggtat agatgatgag
    16381 tttttaagag accaacaaga gaaagattta gcaatgcaaa actatcaaag taactttgct
    16441 aaaaagacta aaatatcaga tgaagaaatg aaaaaatact atgatacaca taaagatgaa
    16501 tttaaaaaag atgaggtaga agcttctcat attttattga aaacagtaga tgataataac
    16561 aagccacttt cagataagga aaaagctgaa gctaagaaaa aggctgaaga ggctcttaaa
    16621 gaagttaaat caggtgagga ttttgcaaaa gtagcaaaaa aatattcaca agatacttca
    16681 gcaagtgatg gtggtaaatt aggattcttt tcaagggggc aaatggttgc tgaatttgaa
    16741 gatgctgctt tttctatgaa aaagggtgaa gtatctgatt tagttgaaac tcaatatgga
    16801 taccacataa ttaaagtgac agacagaata aatgagcaaa catcatttga agatgctaaa
    16861 gaaactataa aagaccaact gttgaaaaat aaatatcaag aacaaataga aaaattaact
    16921 aaagaagcta aggtagaaaa agatgaaaaa gtaataaata aaatcacaat ctaagactta
    16981 aaaaaagagt aatatgctta aaaaagctat atctggataa gatatagctt tttttgtata
    17041 tttttcttaa caatggtaaa aataaactta aagtaattta tggagggatt aaaaaatatg
    17101 aaagcaacag gtatagttag aagaatagat gatcttggaa gggtagttat cccaaaagag
    17161 ataagaaaga cattaagaat tagagaagga gaccctttag agatattcac agcaaaagat
    17221 ggagaagtca tacttaaaaa atactctcca ataggtgaat taaatgaatt ttcgcaagaa
    17281 tatgctgaaa cattagctga agtactagga tatggagtgt tgataacaga cttagattct
    17341 ataatagctg tgtctaagct tcctaaaaaa gattataaag aaaaaagtat aagtgatgag
    17401 ttagaagcaa taataagtga aagaacagta aaatattcaa aagataaaaa cattttacca
    17461 ttattcaagg aagaccaaaa agaatatact agtcaagtta tcatgcctat aattagtgca
    17521 tctggtgact gtataggttc aattgtagtt gtttcaaagg ataaagatac aattgcagaa
    17581 agtgaagaaa aatctttaaa aattgctgct aactttttag gaaaacaagt tcaataattt
    17641 tatcataaag accttttaca tgaaggtctt ttgaagtttt tgtgtaaaat attgaatata
    17701 gcattaaaaa tatggtatat taaagtttgt tatataaaca gtatggaggt tatgttatga
    17761 acaataacaa aaataaagac tcctttctaa aaggagccct tatacttggg ctagcaggtg
    17821 taatcgtaaa aataatgggt ggatttttta ggattcccct tggaaacatg attggttcag
    17881 aaggaatggg atactatcag gctgcttatc ctgtgtatac tctattttta acattagcta
    17941 ctgctggatt tccaacagct gtggcaaaat tggtttcgga aaaagttgca attggaaatt
    18001 ttaaaggtgc aaataagata tttaaggtat cacatacagt tttattcatt accggtataa
    18061 tatcattttg tatattattt tttggagccg actacatagt aacaaacgta atgaaaaatc
    18121 caggtgcgtt atactctatg aaagccattg ctccagcatt actatttgtt cctgcaatgt
    18181 ctgcatatag aggatatttt caagggagac aagatatgac taagattgct gtgtctcaag
    18241 ttgcagagca attttttaga gttgtattag gtcttacttt ggcctacttg cttatgaaga
    18301 gtctaggtca gacttttggt gcagcaggtg ctatatcagg ggctacaata ggttcaatag
    18361 catctatgct ttatttggtg tttgcttata tgctagggag aaaagagcgt agagcagaga
    18421 tagatgcaag ccaaagattt aaagatgaaa gagtgtctta tatttttaaa aagcttttaa
    18481 ctgttgccat tcctataaca ataggagcat cagtaatgcc acttgtaaat atgatagata
    18541 atgttattgt aataagaaga cttatggaag caggatttac atacaaagtg gcaaattcaa
    18601 tgtttggtca attaacaggt atggcaatgg ccacaataaa tttaccagca gtcataacaa
    18661 ctgcaatgag tatgagctta gttccagcaa tttctaaagc atatgcatta ggaaataagt
    18721 caaaagctag aaaagatact aagagtgcag ttaaggtaac tttacttata gtattgcctt
    18781 gtgcgtttgg tatggcttct ttagcaatac caataatggg tcttttattt ccacatgaac
    18841 catcttctgt aggaactatt ttgtttactc ttactccatg tgtattattt ttaggattaa
    18901 tacagacatt gactggtata attcagggaa tgggaaaacc aatagtacct gtaattgctt
    18961 tatgtgttgg tatgctatgt aaaattgtaa taagttatac tttaacaggt ataccagata
    19021 ttaatgtact gggttcagca tttggtacag ttacagcata ctttgtggca gccatgatta
    19081 acttactata tgttaaaaaa cacatgaatg taaacttttc taaaaaagaa tttataataa
    19141 agccatttat aacagttatg acaatgttta taatggttaa gttgagttat ggtgctttag
    19201 ttggattctt aggaaacagc atatctacaa taattgcaat atgtgttggt ggaattgtat
    19261 atgtagtagt aatacttgga ataggtggaa ttaaaaaaga agagattcta actatgccaa
    19321 aaggtgataa attatataaa ttgttaaaaa aagttaaact tataagataa tataggaata
    19381 tatatgcaaa taaaggaaat actatacaaa tagtaaatct ccttatttgt ataataatat
    19441 aaaaataaag tgaaatttgc taataggagt gatgttatgg gtaagataag tattgtaggt
    19501 cttggaccag gagactattc tcttataagc caaggtgtgt tagaatcttt aataaatagc
    19561 cctaaagttt tttttagaac agagaaacat ccaataattg ataaacttaa agaaactata
    19621 caatacactt ctttagacta cttttatgaa aaagaggaaa attttgataa tgtttatttg
    19681 aaaatatctg aatttatcat agaagaatca gcaaagggtg acctagtgta tgctgttcct
    19741 ggtcatccaa gagttgcaga gaagactgta agtataatag agagattagc caatgaaaag
    19801 cacatagcag tagaaatatt gccttctatg agctttgtag acgctatgtt taactatttg
    19861 gctatagacc catctgaggg atttaaactg gtagatgctt ttgaaataga aaattcatat
    19921 atagacttgg atacaagcat gataataact caggtatatg ataaatttat agcatctaat
    19981 gtaaagttaa aacttatgga acattatgac tataatcaag aagtatgtat agtaaatgga
    20041 gcaggtgtca aagatttaga aagtaaaaag tttataaagc tttgtgaatt agacagaaat
    20101 gaaaactttt ttgactattt aactagctta tatataccca aaagttctaa aaagatgtat
    20161 aatacagtac atgacttaga aataatagtt aatacactta gaagtccatc tggatgtgaa
    20221 tgggataaaa aacaaacaca tcaatcatta aaaaattctg ttatagagga agcatatgaa
    20281 ctttgtaatg caattgataa taatgatata gatgaaatgg tagaagagtt aggtgatgtt
    20341 cttttacagg ttatattcca ttgtcagata ggaaatgagg agggctactt tgatttaaaa
    20401 gaagtagtaa atggcatatg taaaaaatta attcatagac atcctcatat atttagtggt
    20461 gcaaaattag atatgtctaa gtttgagaaa acttgggaag aattaaagaa agaagagaaa
    20521 ggcgaaagta ctataacaga tggccttaga aggataccaa aacatttacc agctcttatg
    20581 aaggctagta aagttcaaca taaagcagca cttgtcggct ttgattggga caatgtagaa
    20641 gatgtattta aaaaaattga agaagaatat caggaattac ttgacgaaca cagggtcggg
    20701 aatataaaat acataaagga agagctaggc gatttattat tttcgatagt aaatctagct
    20761 agatttttag acatagactc aggagaagcc ttgaactgta ctacagaaaa attcataaat
    20821 agatttgact ttatagaaaa aagtgcaatg aacttgaata aaaaattaga agacatgaca
    20881 cttgaagaga tggatgagtt ttggaatcag gctaaaaaac aataaaattg tggaaggaaa
    20941 tttgtcaaaa atctagaact tttatcacaa agatagaagt ctatattata aacattttta
    21001 ggaggtaaat atcgtgaata aagctgaatt agtatcaaag atggcagaaa aaagtggatt
    21061 aacaaagaag gaagcagaag ctgcgttaaa cgcatttatg agttctgttc aagatgcact
    21121 agtaaataat gaaaaagttc aattagttgg atttggaaca tttgagacaa gagaaagagc
    21181 tgctagacaa ggaagaaatc caagagatcc agagcaagtt atagatatac cagcttctaa
    21241 agcaccagtt ttcaaagctg gaaaaggatt aaaggatata ataaatggat aattagaaat
    21301 cttgtgaaaa agtgtggata aatttgccac actttttcta tagaaccaag gaaggtgata
    21361 attatgagat tagataaatt tttaaaagta tctaggatta taaaaagaag aactgttgct
    21421 aaagatgcat gtgataaagg gattgtcact ataaatggaa aagttgctaa atcttcttca
    21481 gaggtgaata ttggagatac attagaaata caatttggag agaagaagat gaagtttaag
    21541 ataaatgaag taaaagagca tgtattaaaa aatgatgcaa aagaaatgta cgaaatttta
    21601 gaataaaatt aatagtcctc tagtatatta atagtagtat tattgaaata tttactgggg
    21661 ggatattatg gaacagaaca taactttgaa agatagatca aagctggtta tttcgggagt
    21721 cgagcacata tactctttta atgacaaaag agtagagctc aaaacttctg taggcgagat
    21781 ggtaattgaa ggcgaaaacc tagatatgag caagctaagt atagacgaaa atataattag
    21841 catagatgga actataaatt ctatggtata tgcaaagcct aaaaagccac aagagagttt
    21901 cttgaaaaag gtatttagat agatgatacc ttttactcaa gatgtaagca ttttttatgc
    21961 tactatttat ggtggaatac tcataggggt gttgtttgat ttttatagag ggctaagagg
    22021 gaattttaaa ttcatcaatt attttgctat aatctttgat gtattgtttt ggtttctagc
    22081 tacggttata atattcgtaa caataaacct tacagaattt tttgatttaa gatattatca
    22141 ttttgtggct ttgtttattg gttttatttt atactataac accataagta aaattgtttt
    22201 atcaattatc aataaaataa ttcgttttgt gagaaattct tttaaaaaag ttacacatta
    22261 catagtcagt tttttaaata atttgtatta cgttataatc tattcactac acctactatt
    22321 tgatattata ttttatatac caaacatatt tatagcaaca agaaaaagta taaaaagaaa
    22381 atcaaataaa aaattaaaga acaagaaaaa atcaaaacct aagaagaaaa aaaataaaac
    22441 taaaaaaagg gtgtaggaaa aatgaactta agaaaaaaat tttcaggaca gattattgtt
    22501 atcagtttat ttttaggaat atcaattttt agtatgatga caggatttgt atttgagtat
    22561 acaaaagcta aggaatataa aaaagaaata gcaagtctaa ataaacaatt aaaaaagact
    22621 gaaatacaaa taaacagctt aaagaaagat gaaaaatcat atgaaggaga cttagaggat
    22681 atagcaagaa aaagattgaa tatggtaaaa ccaaatgaaa cagtttatgt agacataaat
    22741 cgttgattaa aacttgctaa taagtttaca gatgtattga ttttaaaatt taagatttaa
    22801 taaaaaagtc attcagttaa ggatggcttt ttgtttttat tgaggaggga atttcataat
    22861 gaaagtagga gctatagata ttgggacaaa ctcaatgaga ttactgatag cagattatag
    22921 agatgggaat tttataaaca gaaaaaaatt tgttaataca actagaatcg gttatggtgt
    22981 ggatagtaaa ggatatataa aagaagatgc tatcaaaaga aatataaaag cattaaaaga
    23041 attttctgat aagtgtaatg atgaaaagtg tgaaaaaatt tattgtatgg ggacatctgc
    23101 tcttagagac agtaaaaatg gagattactt tgttaaatta gctaaagaag aaactggaat
    23161 agatgttgat atagttacgg gaatagaaga atcaaatttg ggctttaagg gtgttttaga
    23221 aggaatacaa ggtgatgata ctattattgt tatagatatt ggaggaggtt caacagaatt
    23281 tattgtaggt gattcagaag gaattaaatt taataaaagt gagaatgtag gtgctcttag
    23341 gatgacagag aggtttttga agcacgatat tattgaagat gaagagtttg aagctatgag
    23401 ttcatttata tatgatgaga ttgacaaaac gttaaatcat ataaaagaca gaggattaaa
    23461 aactcttata ggtataggag gtacgattac atcattatca gcaatgaatc aagaattaga
    23521 gatttactct atggaaaaaa tacataatag taaaatacac aaaaaagaaa tagaagaaat
    23581 tctacaaaaa ttaataaaga tgacattaag tgataaaaag aacttaaaag ggctacaaga
    23641 aaaacgtgcg gatattataa ctgctggtgt aaaaatattg aatataataa tggaaaaatt
    23701 agaaatagaa gaaataaccg taagtgaata cgataatttg gaaggcttaa tatgtcaaaa
    23761 gtcaaaaaaa atgtattaat attttttgat ttttaaaacc gattatgaca aaaaaaatta
    23821 ctcctctttg ttacaattct taatataaac aaaagaggag gtttttttat gcaaagaagt
    23881 gtagcagttg tgaagaataa gactgtaaaa ccaaatgtca taaaattaat aagcggatat
    23941 attgatttta ctacggcggc attcgctgtg atgggatttt tacttagtag gtctattata
    24001 gtagactctg tagctccttt aggaattgcc ttttttatat gtgcatcaaa gattgataag
    24061 tacaagatac ctgtattcat atcaacatta ttaggaatat tgttatcatc taatgatttt
    24121 ggaaatgtag caaaatatgc tgtgtgcctt acaatattta tggctatgaa taaaaaatta
    24181 aaattatcag attcaattgc taaggttgca tttataggtg caataatact acttccaatc
    24241 tccatgggac aagctttctt ttcaaatagg tatgtatatg gtcttcttat agcaggagta
    24301 gaagcaataa taatgtttat atctatatat gtgttttctt atgggatagg tttaatggcc
    24361 aatgtcaaca ataggatgtc tgtacggaca gaagaagcta tatcaataag tttactgcta
    24421 acctttagca taatgggaat aggagatata tctctgtttg gaatatcaat aaggaccatt
    24481 ttatcgaccg tattgatatt gctgacctca atagtaggtg gagctaccat gggagcatct
    24541 agtggtgtaa ttattggtgt agcatccatt ttaaataata tgacatcagc tgtttatatg
    24601 gggatatatt ctttttcagg attaataagt ggagcattta ataaaataaa taaatatttc
    24661 tgtatactag gatatattct aagttggaca ataatatacc tatatacttc tggtattact
    24721 tctaatatga tgcaattaag agatatacta ttaggttgtt tgatagtact agtattgcca
    24781 gagagattat ttaataagat tgaaaaatta ataaaaagta atgtggcttc aaatgaaatc
    24841 gtatacgatt atattatgag aagcaaaaac ctaactaata gcagattgaa cagtatttat
    24901 aaaacttatg atgaccttgc agatacattt gataagataa gagaaaaaga caaagtgcta
    24961 gaccaaagag atatagctaa tgtaatagat atgattcata atgatgaatg taaaagttgt
    25021 agtatgagaa gaatgtgctg ggagtcaaga tttaatcata catacactat gatatataac
    25081 attcttgaaa aaattgagga gaaaggggag ttaagtctaa acgatatacc taaaaatttt
    25141 agaaaggaat gtatgaaacc agagtcgata gtgaaaatat caaatcacta ttacaaaatg
    25201 tttgtattag attatgattg gagtgtgaag ttttcagaaa gtagaaagtt aatagcaaat
    25261 cagataagaa gtatttcaaa atccattaaa tccttatctc aagatttaga aggtgacata
    25321 atgctagata tagaaaagga aaaaaatata tatgaacagt tagaaagata tgatataaca
    25381 gttgataaag taagttactt aacaaaaagt aacaatgagt ttgaaatatc aatagaaaag
    25441 aaaacttgtc atgatggatg tatgtgtgaa gataaaattg taaatataat ttcagaccta
    25501 gtaggtgaaa atatgtctgt tagaaaaata ggatgtcact gtttaggagg taaatgcaag
    25561 gcaacattcg ttaaatctca aaaatataag gctgttacag aagtttcagc aatgtcaagg
    25621 gatgggcata tcctttgtgg agacaattac acatacatgg agataaatga tggtaaatac
    25681 atgatggcta taagtgatgg tatgggaaag ggtaaaaaag catatgaaga atcttctgcc
    25741 actattgata tactggaaaa aatgatagat gcaaaaatta aagatgagat agtcattgat
    25801 actataaata atatgttgtt gttaaaatct tcagaagaga tgttttcaac tctggatttg
    25861 ggaattttag atttaaaacg aggttgttta gaaactataa agatgggagc gtgttctact
    25921 tatataaaaa gagaggatgg agaagttgac ttaatatcat catcatcatt acctgtgggt
    25981 atattatcag atgtaaaaat tgatagaaaa aatgtaaaag ttaaggaagg agactatgtt
    26041 ataatggttt ctgatggaat tgtagatgca ggaagaaata acaatttagg agacaattgg
    26101 ttaatatact tcttaaagaa tatagagact acaaatccaa aggaaatttc aaatttaata
    26161 ttggatagag cattagagtt acaagcgtta caaatcgaag atgacatgac tgttttagtt
    26221 acgaaaatat gtacaaaata aggtttacaa gattatgtgt ataagatgtg gataacttgt
    26281 gtataatatg ttaatagact tttgaaaaaa gtatagttat taacaaaaat aacatataaa
    26341 tcaacaaaat ttgttgataa gtatggcaga aatgttaata aaatgtaaaa aaagaaaaaa
    26401 caaaagctat tatcaaaaga gattcatgtt gaaataacta aatttctaag taaagaaaac
    26461 ctgtgaatat gttgattatt atgtggataa ctattaattc gatatttatt tatatagtat
    26521 gaataattga ataatatatc taaaaaaagc catcctatta ttaagtaaat ttattgaaag
    26581 gatggctttt tatgaataga aaattgtgca aagcaataat acctttattt atttctattt
    26641 tttgcatgtt aataataagt ataaaagctg accaaggata tcaaaagaat aatgacagag
    26701 caattgctgt catgaataat aatgatactg aagatgatgc tgaaaaaaaa caagaagtag
    26761 actatgaaaa aacatgttgg aatttgcaat ctttgttcaa aagtgatgac caatggaaaa
    26821 aagaacttaa gagttttgat aaagatacta aagagttgaa aaattacata ggtaaagtaa
    26881 caaaatcgcc aacacatttg tcttttgcct taaacattaa ggaaaaactt gatataagac
    26941 taaataaact ttctgcctat gtaaaattaa aacaagatac taataaaaat tcatataagt
    27001 acttagatat gagtgatagt atgagtaaat catatgggaa ttatatgggg atttgttcag
    27061 atttagaatt agaaatacta aaattatcaa acaaagatta caagaaaatt atttcaaata
    27121 aaaatataaa taggaaatat ggtgcatatt taagagatat aagaaggaat aaagtacatt
    27181 atttggatga taaatcagaa gatattctaa gcaaagtatc ttcaataagt agtcttccaa
    27241 gccaagtata tgaattattt agaaatatgg atagaaaaac taatttgaca ccagcgcaat
    27301 atgcaagtga attagagtca gtagatagag aaaatagaaa aaaagcttat caagatgaac
    27361 ttataactta caatgataat ataaatacaa ttgcaggttt aattactggt caagttaaaa
    27421 agaatatttt ttattctcag gaaagaggtt atgagtcttc tttagaaatg tacttggaat
    27481 ctgataatgt agatgaaaaa gtatacaata atttgataag gactgtaaat agtaatatgg
    27541 acagtctaca caaatatata gatttaagaa aaaaagtatt aaaattagat aaggttcatt
    27601 catatgatat gtttgtgcct atggttaagg ctgttgataa taatattaat tatgaaaagg
    27661 cacaaagtat agtatattca gcgttgtcac ctttaggaaa agaatacaat gatgtagtat
    27721 ataaagcttt caatgaaaaa tggatagatg tttattcaca tgaaaataaa gtaagtggtg
    27781 gatactgctt atctgtttat gaaaatcatc catatatact tttaaattat aataattcat
    27841 taggttcagt ttcgacttta tcacatgagc ttggacatgc tgtatatgaa tacttaagtt
    27901 ctaagaatca gaattatttt aattcaaatc cgtctatatt tactcacgaa gttgcatcta
    27961 caacgaatga agcattactt tacgagatgc ttataaaaga agctaaaggc aatgatgaaa
    28021 aagcttatta tataacacag tatttagact tgataaaaga tactctttat acacaaacta
    28081 tgtatgctga atttgaaaaa actatacacg agttagtaga aaaaggaaaa agtgtaaacg
    28141 cattagtatt aaatgatgtt tggggacaac ttcttaaaaa atattatgga caaagctatg
    28201 aattagacca attatctaag gtcggatggg ctagaatccc acatttttat aatagtttct
    28261 atgtctataa atatgctaca ggttgtagtg caggagtaag ttttgcccaa gacatcctaa
    28321 agaatggttc agataactat atgagctttc ttaaaaaggg aagttcagat tatccaataa
    28381 acttgttaaa atcaagtggc ataaatttaa ctaatacaaa acccatagaa aatacaatca
    28441 aaaaatttga tagtttagta gcagaacttg aaaagctatt ataaacatta ttgtaatcca
    28501 catggtaaat atatcgaatt aatgataaaa tatactaaaa gtttaaagtc ctatcaagat
    28561 aggacttttt atttattaat tagtgggaga tgattctatg caagttaaag ttaaaaaggc
    28621 tgtaatacca gcagcaggtt taggaactag atttttgcca gctacaaagg cacaaccaaa
    28681 agagatgtta ccaatagttg ataagccaac tctacaatac ataatagaag aagctgtggc
    28741 ctcaggaata gaggaaatac taataataac aggtagaaat aaaaaatcta ttgaagacca
    28801 ctttgataaa tctgtagaat tggaacttga tttagagaaa aaaggtaaga aagaattgtt
    28861 ggaggtagta caaaacatat ctaacatgat taacatacat tatataagac aaaaagagcc
    28921 aaaaggattg ggagatgcaa tttattgtgc gaggcatttt ataggtgatg agccgtttgc
    28981 agtaatgtta ggggatgata ttgtagataa tgatgtacct tgtctaaaac aactaacaga
    29041 tgcatatgag gaatatagaa ctacaatatt gggtgttcaa aaggtaaatc aagaagatac
    29101 taataaatat gggataatag aagcaaaaaa tatagaaggt agagtttaca aggtaaaaga
    29161 catggtagaa aagccagagt caggaaaagc tccttcaaat attgctattt taggtagata
    29221 tataataact cctgaaatct ttgatattct taaggattta ccaccaggta aaggtggaga
    29281 ggtacaactt acagatgctt taaaaatatt atctaagaaa gaagctatgt atgcatacaa
    29341 ttttgaaggc aagagatatg atgttgggga caagctagga tttttagaag ctactgttga
    29401 ctttgctcta aagaaagaag accttaagga agattttata aaatatttga aacatgtatg
    29461 tagtgaattt gataaaaata acaatgtgtt atacaataat acagatgaaa aaatagattt
    29521 aattgaagtc aatgaagcta gagaacttga aattcaaaaa taattaatga aaggtgatgt
    29581 atggtggcaa aatcaagtca gaaaagaatt aatagaataa tagctgttgg gattgtggga
    29641 atcctcgtaa taacgagtat aacagctgtt ttgagtatga ttgcaactat gtaataagat
    29701 aaatgacagc agttgatata cataaatata aaaaccatat ataacttgat gtagtcaaaa
    29761 acttacaata ttttagttat atatggtttt tagaatttat aaatgtaaaa aaaaattttt
    29821 ataatagttg catattttaa aggaaaatat tattataaga tataaatata gtgataaatt
    29881 tataaaaacg tgctaagatt ggacttgaaa tacagtaata tagtaggtaa taatgtttta
    29941 aattttattt attgaccata atatataaat aatgatatta ttagacttaa ctgtttaaat
    30001 aaaacttttc tttttcatgc cttcaatttc caaaataagt atgtatataa atgaatatat
    30061 agatgaggag gatttttttg attttagatg ataaagtatt agaaggatta ggtagtaaga
    30121 ccttagttga gctacgagag atagctaaag aattaaaaat aaaatcaatt actacatata
    30181 agaaaaatga gttaattgag attataaatt ccaaaagtaa aaatgaggaa aaagtaaacg
    30241 aagagactaa agaaaaaaat gaagatatta aagaagacat aaagaataga aaagaaaata
    30301 tagagcgtga aagtatagaa aaagaagttg aatataagag ccctaccaaa tcatacaaca
    30361 agaaagaaat agatacccaa aatatttccc aaaaccaaga aaatagaaat caaagaaatg
    30421 actataataa gacaacgagt gtaaatgata gtaataagtc atcaaatata tctaatagaa
    30481 tggtcagaaa taataataaa aactattata tgcctaaaca agttgatgaa tctaagatag
    30541 tagatgaatt taacacatca aaagaggatg aagtagtagg tgtacttgaa attctgccag
    30601 atggatttgg ttttttaaga ggttcaaatt atttgtctac tgaaggagat gtttatgttt
    30661 caccatctca aataagaaga tttaatatga aaacaggaga caagattaag ggaataacta
    30721 gacatccaaa aagtggagag aaatttagag cacttttata tgttcaaaaa ataaatgatg
    30781 aaaaccctga cacagctata caaagaaatg cgtttgagac attaacacca atatttccag
    30841 aagaacgact tacactggaa actaatagaa atgaaatagc tacaagaatt atagatttaa
    30901 tttcacctat aggaaaggga caaagaggat tgatagtagc acctccaaag gctgggaaaa
    30961 cagttctttt aaaaagcgtt gcaaatagca tagctaaaaa tcatccaaat gttgaactta
    31021 tagtactcct tatagatgaa agaccagagg aagttactga tatgaaagaa tctattgaag
    31081 gagacgttat atattcaact tttgaccaag tatcaagtca tcatgtcaag gttgcagaga
    31141 tggtgttaaa tagagctcaa agattagttg aacatggtaa agatgtagta attttactag
    31201 atagtataac tagattggca agggcatata accttacaat atctcctaca ggtagaactt
    31261 tatctggagg tattgaccct ggtgcacttc atggacctaa aaagttcttt ggtgcagcaa
    31321 ggaatattag acagggtggt tctttgacaa tattaggaac agcattggtt gaaactggtt
    31381 ctaggatgga tgatgtaata tttgaggaat ttaaaggtac aggaaatatg gagctacatt
    31441 tagatagaaa attagcagaa aaaagaatat tccctgcaat agatatttac aagtctggta
    31501 caagaagaga tgatttattg cttgatgatg aagaaaagac tgctctttgg agattaagaa
    31561 gagaaatgag taataattca gtaatggaaa ttacagataa agtcattgaa cttataaaga
    31621 gaactaagga caataaagag tttgtaaaat caataaagaa tttgtaaaat caataataca
    31681 aatgcaaata tggattatat aaactaatag aaaaatcact tgaatggcat aataagttgt
    31741 gctataataa tgtagttgat aatgtaaaaa gaaagttaat ataattaatt tttttaaata
    31801 aagagaagag gtgactataa tgcaaaagga aatacaacca aaatacaatc cagttgaagt
    31861 gcgttgtgct tgtgggaaca catttgttgc tggttcaact aaggacgaaa taaaagttga
    31921 aatatgttca gagtgccatc cattctatac aggaaaacaa aagaacatag aaaaaggtgg
    31981 aagaatcgac aaattcaaga aaagattcaa aatggactaa tttttataaa agtggagttg
    32041 gaggaagcca actcctattt tattacaaaa aaacattcat attctacttt aaattgtctt
    32101 tttgtattat aataataaat atgtaaaatt gagtattcta aatacaataa aatgaggtga
    32161 gtggatggca aaacaagctg taggtgggca agctgttatt gaaggtgtaa tgatgcagtc
    32221 aaaagacaaa agagcggtag ctgttagaaa aagtgacgga gaaatagcac taaaagaaga
    32281 tagaataaaa agttgggtaa gagataagaa tatagataag ataccttttg ttagaggttc
    32341 ttttgttatg atagatacaa tgattcaagg gataaaaagt ttaaactttt cttcagagtt
    32401 ttttatggaa gaagctgaag aggataaatt tgatttattt ataaagaaaa tttttaaaga
    32461 taaggcaaat gatataatta taatattttc attggtcata gcaatgttac tttctgctgg
    32521 attattcatt tttataccta cattggtagg aggagctttt tctaaagtaa tgcctaatga
    32581 ttttatgctt aatttaatag aaggaatcat aaggatagct atattatttg catatatagt
    32641 attgatttca cgaagtaagg atatagaaag agtctttcaa tatcatggag cagaacataa
    32701 atctatatat tgctatgaaa atgatttaga acttacagta gagaatgcta gaaaatttaa
    32761 aagattacat cctagatgtg gtacaaattt cctatttata gttatggctg tatcaataat
    32821 attgtttgca ttttttggtt ggccaaatcc aattcttaga atatttatga gaattatatg
    32881 tgtgcctata gtagcaggtt tatcttatga ggtaattaga gttcttggga aatatgataa
    32941 tggatttact aaaattattg catatccagg aatgatgttg caatatttta caacaaaaga
    33001 gcctgatgat gagcaattag aggttgcttt ggaagcattg aaagcagtag tagattagag
    33061 aaaaggatat ttgttatgac gataaaagat ataattatta aatattcaga taaactaaaa
    33121 gatataagtg atactccaag attagataca gagttattgt tacaaaaaac attgggtgtt
    33181 gatagattat atattcactt aaatctgaat aaagagctaa ctgaagaaca aaaaacaaaa
    33241 tttatgggtt ttgcagaaga aagattaaat ggaagaccaa tagcatatat agttgaaaat
    33301 agggagttta tgggattaga tttttttgtt aaggaaggcg tattgattcc aagaccagat
    33361 acagaaactt tagtagaaga aataattgaa atatgtagag aaaaaaaaga tgtaagtata
    33421 ttagatatag gaactggttc tggagctatt acaataagtt tagcaaagta cattgaaaat
    33481 tctaaaatca tgtcttttga tatatcagag acagcattag aaatagctaa aaaaaatgct
    33541 attataaatg aagttggtga aaaaataaag tatattaatt ctgatttatt tacagcaata
    33601 agtgatagta acataaaatt cgatatcata gtttcaaatc caccatatat taaaaaacaa
    33661 gatatagaaa ctctgcacaa acaagttaaa gattatgaac catataatgc tcttgaaggt
    33721 ggagaagatg gattagactt ttatagaaga ataacagaac aaggtaagaa atatttaaat
    33781 aagtgtggaa tattagcata tgaagttggt cataatcaag ctgaagatgt aataaatatt
    33841 atgaaaagta atggatataa aaaaatatac acaaagaagg atattcaagg cattgataga
    33901 gttgttatag gttacaatat atgatataat caacaattga tatgttacat ttaatcgagg
    33961 tgaaagtatg ttaaaaaaat tagaggtatt ggaagataca tataaagact taagtgaaaa
    34021 aataggtgat ccggatgtaa taaatgatca aaaagtttgg cagaaatata taaaagaaca
    34081 tgctgacttg gaacctattg ttatgaaata cagagagtat aaaagcgttt tagatagtat
    34141 aaaagaatca aaagaaattt tacaagaaga gtcagatgaa gaattaagag aattagctaa
    34201 aatggagtta gctgagatgg aagaaaaagt agcacctctg gaggaagaaa taaaaatact
    34261 attattgcct aaagacccta atgatgataa gaacgttata gttgaaatta gaggtggagc
    34321 tggaggagat gaagcagctc tatttgcggg tgatttattt agaatgtact caaggtatgc
    34381 tgaaagaaga agatggaaaa tagaattatt gagtgcaagt gatactggag ttggtggata
    34441 taaagaagta tcttttatga taaaaggtaa aggtgcttat tcaagattaa agtatgaatc
    34501 tggagttcat agagttcaaa gaataccatc aactgaatct ggtggaagaa tacacacatc
    34561 aacttctact gttgcagtat taccagaagt tgaagatgta gaggttgaaa taaatccaaa
    34621 tgatttaaga atagatgttt tccgttcttc gggtaatgga ggacaaagtg tcaatactac
    34681 tgactctgct gttagggtaa cacatatacc tacaggtgaa gtagtatctt gtcaagatgg
    34741 aaaatcacaa ttaaaaaata aagagcaagc tttaaaaata ttaaaagcta gactttacga
    34801 taaagcattg gcagaacaac acaaagatat agcagcagaa agaaaaagcc aagtcggaac
    34861 aggagacagg tcagaaagaa taagaacata taatttccct caaggtagaa taagtgacca
    34921 tagaataaat ttaactttat ataagttaga tgcattttta gatggagata tagatgaaat
    34981 gatagatgct ttaattactg ttgaccaaac agaaaaaatg actgcaatat aagtaatgtt
    35041 ttaaagaagc tgtttcttcc tagaaattat tttctatttt gaaacagcta ttttgcattt
    35101 taggtcatgt tatcatattc tattgtatat aattgttata tacaagtata ggagggttat
    35161 atgatactaa aagtaacaat aattgggctt ttggcaggtg ttatagggac aggattagga
    35221 ggagtaatat cagccatttt taaaagagag gtcgataaat atctaagttt ttttatggga
    35281 ttatctggtg gaataatgtt ggctgtagtg gtgtttgatt taatgaaaga atccatggac
    35341 aaaatgggga taatcaatac agttatattt acgtttgttg gagctctcat aactatgtac
    35401 ataaaaacaa aattagatgt gtctggaaat atggcatctg gatatcttat attcataagt
    35461 atattactac ataatttacc agaaggacta gccattggtt cttcttttat gtcaactgaa
    35521 agtttaggta ttacacttgc tatagttata ggtctacata atataccaga agggttagct
    35581 atggcattag gcttagtttg caataaaatg aaactaagta aagttatttt gtttactgta
    35641 atagctggat tacctatggg tctaggaagt tttttaggtg tttattttgg aggagtattt
    35701 acttctctta taggagtatt tcttgctaca gcaggaggta ctatgatgta tgttgtattg
    35761 gaagaaattt ttccacattc aaaaagtgtg tattgtataa ttggtttctt gttaggtaca
    35821 atgattgtaa attatatata aaattaaaat gaaagcaggc atacaaaaga tgaaaactat
    35881 tataagtaat attgatataa ataatattga ttatgaggaa ataaaaatac aagcaaaact
    35941 tcttagagaa ggtaaaacag ttatttttcc aactgagaca gtatatgggt tgggtgcaaa
    36001 tgcattagat gaaaatgcag taaagaagat ttatgaagct aaaggaagac ctagtgacaa
    36061 tccattgata gtccatatat atgaaaaaga agaagtatat gacttagcca aagatataag
    36121 tgataaagct aaacttgtta tagaaaagct ttggccagga cctataacaa ttatattaaa
    36181 taaaaaagat ataattccat acaaaactag tggagggctt gatacagttg caataaggat
    36241 gccttctaat gttattgcca gggcaataat aaaagaagct ggtataccta ttgcagctcc
    36301 ttctgcaaat atctcaggtc gcccatctcc aacaaaagca aaacatgtat atgaagagat
    36361 gaatggaaga gtggatggaa tagttttagg aggagacagt aattttggat tggagtcaac
    36421 tgtattggat ttaacagaag agactcctat gatactgaga ccaggaagta taactaaaga
    36481 agttttagag gaacttcttg gagaagtaaa gcttgaccca tcattaagta aaaaagaaga
    36541 taatcaaaaa gctaaagctc ctggaatgaa atataaacat tattctccaa atgcagatgt
    36601 atatataata tctggaaaaa gagaaaatgt agcaacaaag attaatgata tgataaaatc
    36661 taatagagac aaaggtctaa agacaggtgt aatgtgtatc agtagaaacc gagagtttta
    36721 tgatggagaa gtaatcgatt taggaaactc tcttgaagaa gttgcaagta atctgtttga
    36781 tgctcttata gaaatggaca aaaagggcgt tgatgtcata tattcagaag agttccctaa
    36841 gactggagtt ggacaagcaa ttatgaatag actactgaag tcagcaggat acaaagtgat
    36901 aaaggcttaa gagggagttg taaaatatga atatacttat tgtgtgtaca ggtaatactt
    36961 gtagaagccc tatggctgag gctatactaa ggaaagcaat taaagaatct ggaagaagta
    37021 ttgaagaata ttcgatatca tcagcaggta tatctacagc aaatggaatg ggtgcttctg
    37081 aaaattctat agaagtacta aaagaaatcg gtatagactt atcaaatcat agaagtaaag
    37141 taattactaa aaaattaatt gatgaatcag atataatact aacaatgact aaatctcata
    37201 aagaaatttt agtacaagca gttccaaaat gcaaagaaaa agtgtatact tttaaagggt
    37261 ttgcaaataa aaatgaggaa gatatatctg acccatttgg tggaaatttg gatatttata
    37321 gaagcactat gagagaaata atgtattctg tcaacgaaat agtaaaaaaa atatgaaacg
    37381 gaggataaac aaatgaagat aggattaggc tgtgaccatg gaggatataa tttaaaaaaa
    37441 gaaataataa gctatttaga aagtaaaggt atagaatgtg tagattatgg aactaataat
    37501 gctacagatt ctgttgatta tcctgtttat ggagaaatag ttgcaaattc agttataaat
    37561 aaagaagtag attatggaat attatgttgt ggtacaggga taggaatatc tttagctgct
    37621 aataaggttc ctgggataag atgtgcagtt gtatcagatg tattttctgc aaaaatgtca
    37681 aaagcacata atgatgcaaa tatgttatca ttaggcgaaa gagttttagg aaaaggtcta
    37741 gcacttgaga tagttgaagc atggattaat acggattttg aaggtgatag acatgctaga
    37801 agagtaaaca tgattaaatc aatagaagaa aaacataata aataataaaa attacagttt
    37861 tatcaggagg aattatcaaa atgagcaaag tggtagagac aaaccatcca ttaatacaac
    37921 ataaattaac tttaatgaga gataaaaata ctggttctaa agactttaga gagcttttaa
    37981 ctgaaattgc aatgttaatg gggtatgaaa taacaaaaga tataccatta aaagatgtag
    38041 aaatagaaac tcctattcaa aaaacatcat ctaaagtagt tgcaggtaaa aaattagcaa
    38101 taattccaat tttaagagca ggtcttggaa tggtagatgg attagtaagc ttaatgccag
    38161 cagcaaaagt tggacatgta ggactatata gagacccaga aacattaaaa ccagttgaat
    38221 attattgcaa gcttcctcaa gacataggag aaagagatat aatagtagtt gaccctatgc
    38281 ttgcaacagg gggttctgct gtagcagcta tagatttact taagtctaaa ggagctaaaa
    38341 gtataaaatt agctaattta gtagctgcac cagaaggtat agcagaagtg caaaaatatc
    38401 atgatgatgt tgatatatat gttgcatcag ttgacgaaag attaaatgaa catggatata
    38461 taattcctgg tcttggtgat gctggagata gattgtttgg aactaaataa aaataaataa
    38521 ctataaatat ttattaggga ctttcttata tagtgtatat aattttatgt aagaaggttc
    38581 ttttttatat attttttctc aaaataggaa ttatgaattg ataaatttct cgaattgaga
    38641 ataaaaagtt tttaccaatt aaaaaagatt gcttatttta caaggtttta tagaatagta
    38701 gtctatttta atgtggcatg ttagttgctt acgattaaga ttataggtat taagtgtatt
    38761 agttaaattg agaattgaat attaataata ttattgggag tggtaataca tgaagaattg
    38821 tatagcagta agaggggctg gagatttagc aacagcagtt attcataaat tacacaacag
    38881 tggttttaaa gtagttgcgt tagaaataga agagccatta gctataagaa ggcaaatttc
    38941 tttttgtgag gctgtttatg aaaaaaaagt aactgttgaa ggtgtagaat gtatattttg
    39001 caatactgaa aaagaaatag aagaagtttt atctcaacat aaagtagcct tgattataga
    39061 cccaaaagga gaaaaattat ctaagttaaa tccaatcgtt ttggttgatg gaatactcgc
    39121 taagaaaaac ttaggtacaa ctaaagatat ggcaccactt acaatagctt taggtcctgg
    39181 attttgtgca ggagaagatg tagatgttgt tatagagaca atgagaggtc ataatctagg
    39241 aagaataata actaatggta gagcagaaaa aaatacagga gtaccaggag caataaaagg
    39301 tttttctaaa gagagagtta tgcattcaca gtatgatggt ataatggaaa atgtatgtaa
    39361 gataggtgat atagttgaaa agggagacct actttgttat attaataatt atgaaaaaaa
    39421 acatgaagtt agagcaacaa tatctggagt ccttagaggt ttattaaagg atggagcaaa
    39481 ggtgaacaaa aatttaaaaa tactagacat agaccctaga aaagaagaag ttaagaatgc
    39541 ttttacaata tcagataaag ctagatgtat agctggaggg gttttagaag ccatactaaa
    39601 caatgggatt tatccccaaa attaatttat attagataaa gctattaata ttttattaag
    39661 atgatataat aaatttaata gattaaagag agcatatgac gggtatttaa aatttaaatg
    39721 gtaaaaatgc tcttttttta ttggacattc aattaacatg tgtatataat ataaaaataa
    39781 gtatttattt aggttgaaat ctaataaata tataatacca gtacataagc aaagaggaga
    39841 aaagtaaatt atgagaccat catgggatga atacttcatg gaaattgcag aagtagtgaa
    39901 aaagcgttca acttgtatta gaagacaagt aggagctgta attgtaagag acaaacaaat
    39961 attaacaact ggatataatg gttctccgag aaatcttgaa cactgtgaaa atattggatg
    40021 taagaggcag gaattaaata ttccctcagg agaaaggcat gaattatgca gagcattaca
    40081 tgctgaacaa aatgcgataa tacaagctgc tcataatggt attagtgtag atggggcaac
    40141 tttgtatgtt actacaagac cttgtgtttt gtgtgcaaaa atgtgcataa atgcaggtat
    40201 attgaaaatt gtttatgaag gtgattatcc agatgatatg tctacagaat tattaaaaga
    40261 agcaggaata gaactcataa aattttagtg gataaaattt atatgacaat aaatgtcaaa
    40321 ttatgacact gaaatacaat ggtatcaaat gattaaaaga aagtatttga aaaatcatca
    40381 gattagtagt aagatatatt ttataattaa ataaatttat gagatagatt aattacatgt
    40441 tatttatttt ataataatga gggatactat ttatgagtaa aagaaataca catacagaaa
    40501 ttgcaaagtt gtttagttta ataagtcaaa taggattgat gattgtaata tcaatattag
    40561 gatgtacttt cttaggaaaa tttttagata gtaaattgaa tactactcca gttctgacaa
    40621 taatattttt attgttgggt gttggaggtg cttttagtgg tgtttataaa actctaatag
    40681 tatatacacg aaggaagtga tatattggat ataaaattac ttaaccaggt aaggtcagta
    40741 acaaaaggta tagtaatatt tgatgtagtt ttaattgtgc taatgttgat aacatcaaca
    40801 ttaagtaaac cagtattatt aggacttata tttggctcta taatagctat gttaaatttt
    40861 agattattgg cgatatcact ggaaaaatca gtgacatttc ctgctgggaa ggcacaagct
    40921 tatgcatcag cacaatatgc tattagatta tttataatgg cagttgttct aattgcatca
    40981 gctacagtac cacatttaaa tataatcgga acagcattgg gattattgag cacaaagttt
    41041 gtcatattat caaaaaactt tataaataaa ttaaagagga aggaggcata agtatgaatc
    41101 aagcgcatta ttatctcata ggaaacttta agattagtga aacaatacta gtaagttggg
    41161 gaataatggc ctttttagcg atagcatctt attttttgac tagaaactta aaaaaagttc
    41221 caactagtaa agttcaaata ttcttagaat ttgcagtagg tggtcttgct aaaatggtta
    41281 cagatgttat gggtaaaaat acagtaaaaa gaatgccata tatgatacca tacataggaa
    41341 gcttgttttt atttttcgca tgttcaaatt taagcccatt acttggatta aggtcaccaa
    41401 caaccgactt agacacaact gctgcatggg caatgattac attttttatg atttattttg
    41461 caggagttaa atttaagggg ttatcatact ttaaagaatt ggctcaacca cttgccataa
    41521 tgttaccaat caatattata ggtgagttag ctagaccaat atcattaagt ttccgtccat
    41581 ttgggaatat tttaggtgga acaataataa tgggtctgtt ttatcaactt ttaggtttta
    41641 tatctagttt aataccagga ttatcaatac caattggaga aatattaata ccagtcccat
    41701 tacacttgta ctttgactta tttgcaggat tgttacaagc atatatattt ataatgttaa
    41761 caatggtatt tatatcaagt gcaggagagt tagattaaga aatcaactta tattaagata
    41821 aaaataaaat aatttaaaaa attaaagttt taaggaggaa ataattatgg aaacagcaat
    41881 agtagcggca gcatcagcaa taggagcagg gatagcagtt gcaacaggta taggtgcagg
    41941 tataggtcaa ggtatagcag cagctaaagc tgcagaagca gtaggaaacc aaccagaagc
    42001 taaaggtgat ataacatcaa ctttattact aggagttgct atagcagagt cttcagcaat
    42061 atatggtttg gttatatcaa taatacttct attcgtaaat ccattcttta aatacttagg
    42121 aatgtagatt tagtccttaa atattttgag ataggtttat aggtaaatca tctcaaactt
    42181 ataatcatga gatgaacttt atcatctcgt gattataagt agttatgatt tatggaaagg
    42241 aggataatta tggaaaaagc actagtaggt ataacgtggg aatttgtgtt ccaaattgtt
    42301 aatactttta taatattttt acttctgaga aagcttttat tcaagccagt attaaatata
    42361 attgaatcaa gagaaaatga tattaaaagt gatttggcag aaggcgaaaa agcaaaaaat
    42421 gaaggtttag ctctgaaaaa agaatatgag tcaaaaatca attttgccaa ggatgaaggt
    42481 caagaaatca taaaacaagc aactataaga gcagagcaga aatcagatga tatagtaaat
    42541 actgcaaaga aagatgctct tgatataaaa gaaaaagcaa acaaagatat agaacaagaa
    42601 agacaaaagg ttataaatga aatcaaaaat gatatatcta acatagcact acttgcagca
    42661 tctaaagtta ttgaaaaaga tttagataaa tctaaacatg aagaattgat agaaaacttt
    42721 ataaaagagg taggggaagc taaatgataa atgtaatagc aaatagatat gccgaagccc
    42781 tatttcaatt aggagaagaa gaaaattcaa ctgatgtctt attcaaagaa ctagaaaaag
    42841 tagtagatat gatgactaaa gttagtaaag atttctacaa agttttaaaa tcaccattag
    42901 tttcaaaatc agaaaagaaa aacttggtag aaattatttt tagtaaggaa gtaagttcta
    42961 acataaaaaa ctttttaaaa gttttagtag ataaagatag aatatcatat ttagaagata
    43021 tcgaattagc ttataaagaa ttattaaata agaaaaacaa tgtaattgat ggagtagcaa
    43081 taagtgctat tccaatgagt gaaacagata ttaaagaact tgaagtcaaa ctttcaaata
    43141 aatacaacaa aaatgttact atagaaaatg ttgttgacaa gactatacta gggggagttc
    43201 tagttagaat tggaaatgaa caaatagatg gcactgtgaa aactcgtctt gataagatga
    43261 aagaaaagct atctgaagta atatcttaag aatggcggtg aacccatgaa cttaaaacct
    43321 gaagaaataa gttctataat taaacagcaa ataaaaaatt atgagaataa agttgagttg
    43381 acagatacag gtagtgtttt aacagttggg gatggtatag cgagtgtata tggattagaa
    43441 aaagctatgt ctggtgagtt gctagagttc ccaggtgaaa tatatggtat ggcacttaac
    43501 ttggaagaag aagtagttgg agctgttatc cttggagatg acagtgaaat aaaagaaggc
    43561 gatatagtta agagaactgg aagaatagtt gaagttccag ttggagaagc tttaataggt
    43621 agagttgtaa actcactagg tcagccaata gatggaaaag gacctatagc gtacacaaaa
    43681 actagaccag ttgaatctga ggcacctggt ataatagata gaaggtctgt ttatgaacca
    43741 ttacaaacag gaatcaaatc aatagattca atgataccaa ttggtagagg tcaaagagag
    43801 ttaataatag gtgacagaca aactggtaaa acatctatag ttattgacac tatactaaat
    43861 caaaaaggaa aagatgttat ctgtatatat gtagcaattg gacaaaaacg ttctactata
    43921 gctcaattag ttagctcttt agaaaaaggt ggagctttgg attatacaat agtagtttca
    43981 gcaacagcat ctgaatcagc accacttcaa tatatagcac catatgctgg agcagctatg
    44041 ggtgaagaat ttatgtacaa tgggaaacat gtacttatag tgtatgatga tttaagtaaa
    44101 caagctgttg catatagaga aatgtcattg ttacttagaa gaccaccagg acgtgaggca
    44161 tatcctggag atgtattcta tttacattca agattacttg aaagagcagc taagttatct
    44221 gatgaattgg gtggaggttc aatgactgcc ttgcctataa tagaaactca agctggtgac
    44281 gtttctgcat atataccaac aaatgttata tctataacag atggtcaaat atacttacaa
    44341 ccagagttat tctattcagg tgtaagacca gcagttgacc ctggtatatc agtatcaagg
    44401 gttggtggtt ctgcgcaaat taaagcaatg aaaaaagttg caggaacatt aaaacttgca
    44461 tattcacaat atagagaact tgcagcattc tcacagtttg gttctgactt agatgaagat
    44521 actaaaaaga gacttgctca aggtgaaaga atcgttgaga tattaaaaca aggtgagcat
    44581 caaccaataa aagttgaaaa tcaagttatg ataatatatg ctgttataaa taatcattta
    44641 gaggatattc cgatagataa tatagctaga tttgaatcag aattgtatgc atttgtagat
    44701 aataattatc cggaaatatc aagaaaaata ttaggtggag aagacttcac tcatgattta
    44761 acagatgcaa taaatgaatt taaagaaaag tttgttgttg aagtataatc ctttttaaaa
    44821 aaggaggtaa attaattggc aggagctgga atcaaagcaa tcaaaacacg tatgggaact
    44881 gttgaaagta caaagcaaat aactaaagca atggaattag ttgcatcttc taagcttaga
    44941 aaagcaaaag aaaaggctga agattcaaga gaatatttta atactttata caatacagta
    45001 aaagatattg cccaaacaac acgtggaatt agaaatgaat ttttaaaaga aagagaagta
    45061 aaaaataaat gttatgtagt agtagctgga gacagaggac ttgcaggtgg atacaattcc
    45121 aatgtattaa aggaatctgt ctctcatatg aaaggtaaaa aggaaacagt gattactgtt
    45181 ggaaagaaag cacatgaatt tttttctaaa agaaactaca atatagcaaa agatataaat
    45241 agtgttgaag cttgtggata tgaagatgcg acagaaattt cacaaactat tattgacctg
    45301 tataaaaaag gtgaagtaga tgaagtttat attgcttata caaaatttaa atctgcttta
    45361 tcacaagaag ttgaaatttt gaaattgctt ccattagtct ttgaaaaaga tacagacgaa
    45421 caaggaatgg cagtaaaaga agaaaaacaa aatagatcaa aagtacaata tataccatca
    45481 gcagaagcag ttttaagtta tattgtacct cagtttatat caggtacagt atatggtgga
    45541 attttggaat cttttgcatc agagcaaggt gctagaagaa ctgcaatgga atcagccaca
    45601 gacaatgcga atgaaatgct atctaattta gagttaagtt ataacagagc aagacagtca
    45661 gctgtaactc aagaaataac agagatagtt ggtggagttg aagctctaaa atagggaggt
    45721 taggaaatgg caaatgtagg taaagtagta cagattgtag gagctgtact agatgttaag
    45781 tttgatagtg aacaaagcct acctaactta ttaaatgcgt tagtaataaa gttaggagat
    45841 aaagaaatag ttgcagaagt tgctcaacat ataggtgatg atactgtaag atgtatagct
    45901 atgagtgcaa cggacggact tgtaagaggt atggaagttg tagatacagg agggccaata
    45961 agtgtccctg ttggagatga gactttaggt agaatattca atgttcttgg aaaaccagtt
    46021 gatgggaaac cagctccaaa atctgcacct aaattaccta tacatagacc agcaccggcg
    46081 tatgatgaac tagaaactac agcagagata ctagaaactg gtattaaggt agttgactta
    46141 ctagctccat atctaaaagg tggaaaaata ggtctatttg gaggagcagg agtaggaaag
    46201 acagttctaa tacaagagct tataaacaat attgctaagc aacatggtgg tatttctgta
    46261 ttttcaggag taggagaaag aacaagagaa ggtaacgacc tttatggcga aatgagtgag
    46321 tctggagtta taaataaaac agctctagta tttggtcaaa tgaatgaacc acctggagcg
    46381 agaatgagag ttgctttaac tggacttaca atggcagaac attttagaga tgagcaagga
    46441 caagacgttt tacttttcgt tgataatata ttccgtttca cacaagctgg ttcagaagtt
    46501 tcagcacttc taggacgtat gccatcagct gttggttatc agccaacatt agctactgaa
    46561 atgggtgcac ttcaagagag aataacatca actaagaaag gttcaataac atctgttcaa
    46621 gcagtatatg taccagcaga tgacttaact gacccagcac cagctacaac attctctcac
    46681 ttagatgcaa aaacagtttt atcaagacaa atatcatcac tgggtatata tccagcagtt
    46741 gaccctcttg aatctacatc aaggattctt gaccctagta tagtaggaaa agaacattat
    46801 gaagtagcta gaggtgtaca atcaatactt caaagatata aagaacttca agatataatt
    46861 gctatacttg gtatggatga attatctgat gaagataagt taatagttgc acgtgctaga
    46921 aagatacaaa gattcttatc tcaatcattc acagttgctg agcaatttac aggaaatcca
    46981 ggtcaatatg ttcctgttaa agagactgta agaggattta aagaaattct tgaagggaaa
    47041 catgatgact taccagagtc agcattctta tttgtaggaa caatagaaga cgctgttaga
    47101 aaagcgaagg gaagtatgta gtcatggcca gtgaattttc attgaaagtt gtaactccaa
    47161 gtaatacttt ttatgatgga gatgttgaaa tgatagtagt tagaactaca aaaggagaca
    47221 aaggggtgtt gaaaggtcat ataccatttg tttcaggtat acctgtagga actttaaaga
    47281 taaaaaaaga gggaagtttt aaagaggcga aaatagcaga aggattcatt aatgtaactc
    47341 aagataaaac tgtcatattg acagaatctg ctgagtggat ttaataaatt aatttagagt
    47401 ataaaaagtc attttgacca aaaaaacttt tgggtaaaat ggcttttttg tgttttggtg
    47461 ttaataaaat aacgataaaa ttgtaagttt ttagtgataa taaattcaca aaatgtttaa
    47521 atttactgcc aaattatgtt gtaattaata agataaagaa gatttagata
//
LOCUS       DAELSY010000010        46429 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.010, whole genome shotgun sequence.
ACCESSION   DAELSY010000010 DAELSY010000000
VERSION     DAELSY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46429
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.010"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            462..1094
                     /locus_tag="KPG09_000499"
     CDS             462..1094
                     /locus_tag="KPG09_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG0965128.1"
                     /translation="MTIPNSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVSPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(1465..2334)
                     /locus_tag="KPG09_000500"
     CDS             complement(1465..2334)
                     /locus_tag="KPG09_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0965129.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(2431..3288)
                     /locus_tag="KPG09_000501"
     CDS             complement(2431..3288)
                     /locus_tag="KPG09_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0965130.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            complement(3503..5521)
                     /locus_tag="KPG09_000502"
     CDS             complement(3503..5521)
                     /locus_tag="KPG09_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG0965131.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLIMERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            complement(5792..6568)
                     /locus_tag="KPG09_000503"
     CDS             complement(5792..6568)
                     /locus_tag="KPG09_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG0965132.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGLIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(6625..7596)
                     /gene="asrC"
                     /locus_tag="KPG09_000504"
     CDS             complement(6625..7596)
                     /gene="asrC"
                     /locus_tag="KPG09_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBG0965133.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(7609..8400)
                     /gene="asrB"
                     /locus_tag="KPG09_000505"
     CDS             complement(7609..8400)
                     /gene="asrB"
                     /locus_tag="KPG09_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBG0965134.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(8401..9408)
                     /gene="asrA"
                     /locus_tag="KPG09_000506"
     CDS             complement(8401..9408)
                     /gene="asrA"
                     /locus_tag="KPG09_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBG0965135.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEIIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            complement(9798..10487)
                     /locus_tag="KPG09_000507"
     CDS             complement(9798..10487)
                     /locus_tag="KPG09_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG0965136.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKAKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITFDSSKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            complement(10550..11398)
                     /locus_tag="KPG09_000508"
     CDS             complement(10550..11398)
                     /locus_tag="KPG09_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0965137.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            complement(11417..12304)
                     /locus_tag="KPG09_000509"
     CDS             complement(11417..12304)
                     /locus_tag="KPG09_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG0965138.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEVWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNINGSSFQELASTSSLIKKVAKIKNLNEKDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            complement(12362..13165)
                     /locus_tag="KPG09_000510"
     CDS             complement(12362..13165)
                     /locus_tag="KPG09_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965139.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDALYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACNECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            complement(13325..13777)
                     /locus_tag="KPG09_000511"
     CDS             complement(13325..13777)
                     /locus_tag="KPG09_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBG0965140.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            complement(13907..15409)
                     /locus_tag="KPG09_000512"
     CDS             complement(13907..15409)
                     /locus_tag="KPG09_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG0965141.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSVVLAGLTGIPVNVLIVV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFIGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            complement(15442..16308)
                     /locus_tag="KPG09_000513"
     CDS             complement(15442..16308)
                     /locus_tag="KPG09_000513"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBG0965142.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            complement(16393..17064)
                     /locus_tag="KPG09_000514"
     CDS             complement(16393..17064)
                     /locus_tag="KPG09_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBG0965143.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            complement(17337..17828)
                     /locus_tag="KPG09_000515"
     CDS             complement(17337..17828)
                     /locus_tag="KPG09_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0965144.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            complement(17850..19214)
                     /locus_tag="KPG09_000516"
     CDS             complement(17850..19214)
                     /locus_tag="KPG09_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0965145.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            19633..20808
                     /locus_tag="KPG09_000517"
     CDS             19633..20808
                     /locus_tag="KPG09_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0965146.1"
                     /translation="MNLEKIKKFFGFPLDKKHKENVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFATPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCDETDAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIEKAITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(20981..22378)
                     /gene="asnS"
                     /locus_tag="KPG09_000518"
     CDS             complement(20981..22378)
                     /gene="asnS"
                     /locus_tag="KPG09_000518"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBG0965147.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(22455..22688)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(22857..23051)
                     /locus_tag="KPG09_000519"
     CDS             complement(22857..23051)
                     /locus_tag="KPG09_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBG0965148.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(23325..24998)
                     /locus_tag="KPG09_000520"
     CDS             complement(23325..24998)
                     /locus_tag="KPG09_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG0965149.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPVDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSQLNIAINRND
                     GSLSQDNIGTGTLVSGILAGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     ISGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(25008..28406)
                     /locus_tag="KPG09_000521"
     CDS             complement(25008..28406)
                     /locus_tag="KPG09_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG0965150.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNTLLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVVTVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIISFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSSINQDLETEDIGYRSINKSFKDEENRYKFIDGYNIQI
                     HNDLENEIYISKNASRQSGILSGIDVVHTPEFEEELAGLGMSQNFFKISDSLGVLSIN
                     NTDYSSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICSGLGNVNSEYAGIAEDSELIIIKLGKIDGFYNS
                     AMLFAASQYAYKKAFELRRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPHVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITVGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(28572..29768)
                     /locus_tag="KPG09_000522"
     CDS             complement(28572..29768)
                     /locus_tag="KPG09_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBG0965151.1"
                     /translation="MFIEFFYLLRSRGLNISLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMLSR"
     gene            complement(29789..30610)
                     /locus_tag="KPG09_000523"
     CDS             complement(29789..30610)
                     /locus_tag="KPG09_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBG0965152.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            31074..32168
                     /locus_tag="KPG09_000524"
     CDS             31074..32168
                     /locus_tag="KPG09_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG0965153.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
     gene            32319..32675
                     /locus_tag="KPG09_000525"
     CDS             32319..32675
                     /locus_tag="KPG09_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965154.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(32768..34036)
                     /gene="eam"
                     /locus_tag="KPG09_000526"
     CDS             complement(32768..34036)
                     /gene="eam"
                     /locus_tag="KPG09_000526"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBG0965155.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(34438..35820)
                     /locus_tag="KPG09_000527"
     CDS             complement(34438..35820)
                     /locus_tag="KPG09_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0965156.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            35945..36631
                     /locus_tag="KPG09_000528"
     CDS             35945..36631
                     /locus_tag="KPG09_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBG0965157.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEGVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            complement(36817..38352)
                     /locus_tag="KPG09_000529"
     CDS             complement(36817..38352)
                     /locus_tag="KPG09_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965158.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFNLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            38627..39676
                     /locus_tag="KPG09_000530"
     CDS             38627..39676
                     /locus_tag="KPG09_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0965159.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENRVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            complement(39725..40759)
                     /locus_tag="KPG09_000531"
     CDS             complement(39725..40759)
                     /locus_tag="KPG09_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965160.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVVWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(40777..41124)
                     /locus_tag="KPG09_000532"
     CDS             complement(40777..41124)
                     /locus_tag="KPG09_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG0965161.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            41382..42764
                     /locus_tag="KPG09_000533"
     CDS             41382..42764
                     /locus_tag="KPG09_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG0965162.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            complement(42845..44638)
                     /locus_tag="KPG09_000534"
     CDS             complement(42845..44638)
                     /locus_tag="KPG09_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0965163.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDGIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILDELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(44891..46282)
                     /locus_tag="KPG09_000535"
     CDS             complement(44891..46282)
                     /locus_tag="KPG09_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG0965164.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
BASE COUNT        15209 a         7920 c         5082 g        18218 t
ORIGIN      
        1 aatgaatcta aatcatttgc attacttcag agttttgact aaagttgaac accatacaaa
       61 agtagctact caattgttta taccccaacc aagcttaagt aatgttatat agattcttga
      121 aaaagaattt ggtatctttt tatttgaaaa acaaggtaga aatgtttccc tcactaaata
      181 cggaagtttt tttctaacct atgttgataa agcattaggt taattagaaa aagtttacat
      241 ataaaatata ttaaattgtt gcaagtttta ttgttttgat atacaatcaa ctaaacatat
      301 aatctttata aaatactata cagttaaaag tctgttttaa aaaaaaatgt ataaattata
      361 aatcaaaata tagatactat caaggagtga ttctgtgaag agatgtattg tagtagtgga
      421 atagtcaaaa acataatata tattaaagga gagatttaaa tatgactatt ccaaattcaa
      481 ataaggtata tccaagaagt aatgattatc aaactgtata tcttaaaaat gtaataacaa
      541 gggaaaatat agaagttgga gattacacaa tatataatga cttttataat gacccaagag
      601 attttgagaa aaacaatgta ttatatcatt accctataaa caatgataaa ttaataattg
      661 gtaagttttg ctcaatagca tgtaatgcaa aatttctaat gactagtgga aatcactcaa
      721 tgaaatcaaa atccacatat acttttccca tcttttatga agaatggaaa ctcgatatca
      781 accatataac ggacgcttgg gacaataagg gtaacattat aattggaaat gatgtgtgga
      841 taggttatga ttctataatt atgtcaggtg taaatattgg tgatggtgca attattggaa
      901 caagagcagt agttactaat gacgtctcac cgtattctat tgttggagga gtacctgcaa
      961 aaattataaa aaagagattt aataatgata caatttcaaa attattagaa attaaatggt
     1021 gggattggcc atataaaaaa attcaatcaa acattaagca tattcaatca ggaaatattg
     1081 ataaattagt ttaatatttt ataatttatt tatattaaac tttactttaa taatcctttt
     1141 ataagagctt tttcaaaatt tgtatattat tttaatatat ataaatttaa tacaacatta
     1201 aataaaataa aaagtaaagt tacttaataa aaagttactt tactttttat tatttaaatt
     1261 cctgttttta ttataatagg taaaattcta ttaaactagt tcacattttc attattattg
     1321 atatattata tatgaatata ccattttgtt ttttaataaa aaaatcatgc tacatcagta
     1381 gtaagcaact attaatgtag catgattttt tgtatctaaa taatcttagt atttacctat
     1441 caatatagat tgatatatca aaaactatag tcttctatat tcttctcttt ttagcttttg
     1501 gttacttaga tagtaatcaa tttcatcaat tagacgttgt ttatcatcag gtaatacacc
     1561 ttttaaatta gcataatttg atgcatgatt acttctaaat acaatttttt cattagcatc
     1621 tatattcttt atcatctctt taatttcttg taacacagcc ttatcctcta gtattgtaaa
     1681 ctctcctgcc ttaagtaaat cgtaaagttc agtattaggt tctaccatta aactaagtac
     1741 acccacataa tctggtgaaa ttgctcctaa catttttcct gtatcaactg catgctgatg
     1801 tgttttttta gttcctccaa gacctgctat tacagtggct gataatttaa tcccactttt
     1861 tttaactttt ctaccaactt ttattaattg ttcactacta aaacctttct taatatcttc
     1921 taatacttca ttgcttccac tttcaagccc tagataaatc attttaacac caagttttct
     1981 taattcttta agttcatcat cagatttttt ctctatagat ttaggagagc cgtacatagt
     2041 aattctttca cactctggaa aaacttcttt tatatataaa ataatatctc ttaatttctc
     2101 cattggtatt atcaatgcat ctccatctgc taagaatatt ctttctacat gcttataata
     2161 tcttctaaat atatcaattt cattttttat tacttctagt ggctttattt taaatttttt
     2221 agatttgtac atactacaaa acgtacattt gttatgagaa catcctagtg tgacttgtat
     2281 tataagacta taagcctcac ttggtggtct gtaaagtggc atatcgtatt ccatattaaa
     2341 atcccccttg taataaaata ttcattctat tatattatta atatttactt taaataataa
     2401 cttataaaat tatatttttt aaattttagt ttaagatata ttctttaaaa atttaatgat
     2461 actattggca gtaatattaa gttgtttttc ttgacttata atagcatcat tatctccctt
     2521 ttgttttcca tagtctccaa attgtgcatg atttcctcct tcaatctcaa cataagttgt
     2581 atcatcagga agtttttgtt ttgattctac aagactttta aaattgacta ctccatcttt
     2641 gctcccccat atagatataa ctttttttcc taattctttc aactcatcac ccattggata
     2701 agaagctaaa aatacaacgc cttcaattaa tttattatca cgtgcaaaat ttgatgctgc
     2761 aactcctcct aatgaatgac ctcctattac ccaatgttct atgttgttgt aagaatccat
     2821 aactttctgt gccttattag ttccaagtat agctagatta agtggcatct ttacgattac
     2881 aacctcatat ccactttctg ctatttttct tgcaagtgga gcataagatt ctatttctac
     2941 tttagctcct ggatacaaaa ttaaaccttt agaaactttt cttccttttg gtgtaaatga
     3001 aatgttttca tttacagtta cttcaacttt actattgctg actagagctt cttttgccag
     3061 cttttgaggt tcatatgttt tatttatcca tatgaaaaag ccaagcaaaa tcaatacaaa
     3121 tagtatacct attcccttaa atatcttatt ctttatgtta aattttctat tattattttt
     3181 atcatctttg ttctcaacct catcataaag taaaggtctc tcaagttcca tagttatact
     3241 aaatgtatca tatttcttat tacgctttac ctttacatct ttactcataa atctaacccc
     3301 cataatattt gaatactatt atagtttatc ctaagtaaat tgttttttca accaatgaaa
     3361 aaaatcaata ttttaactat tttcatataa aattcataaa tatatgtttt ttgtttttta
     3421 tatattattt tcttttagta ttttataaaa caaatagcca tatactttat tttaaatata
     3481 tggctattct ataatttaat atctaagctg cttcttttat tgaaacttta gttatattac
     3541 ttattctata catttctttg acaccattta gagtttgcag taagtttaga catattatct
     3601 tgtcacctag acttttagta tttacaatac tctctaaaat tacacttctt ccttcatcat
     3661 acttatctac attgtaattt gaaatataat tagtatctct caatattttt tcaacatctt
     3721 catcttttat tttattttta ttaacattta tatatagatt atacatatta cttattagta
     3781 atttctgatt ttctaaaaaa tcctcttgat gtttatcttt atatatagca tttatcaaca
     3841 taagtctatc ttcaataagt gatggtatca ataataaatt ttttatatag taaacatcag
     3901 ctacattttc tatagactta ttgacttcat caattatatt tttaactatt cctttataca
     3961 tttctataac atgttgatag cctgttttag catcatatgg taatataaac ttatttacta
     4021 ttaatgcaat aattgctcct aatgctacat atgaaatcct gcttatggtt aaaacatttg
     4081 ggtctcccat aactactgct gaaccaagag ctgaaacagt tacacatacc attaattttc
     4141 tataatcaac tacataagag tttatatacc ctgctaaaag aactattaac gaccttaatg
     4201 tactatcttt tattattgaa aataatacaa taaatattac agctcctata aatgtacctt
     4261 ctattctttt tctagacctt tgtatacaat tttctgcgta aggttgtgtt aatgaaaaga
     4321 cagttaacat tatccatctt ccatctctta aatggaaata atccataata aaacccgcta
     4381 cagctgtagc taaagcaatc tttactgcat aagaaaatct tatagaattt aaattaaagt
     4441 ttttcttata tattgatagt cgtttaaatt catgaggtat ctttgatttt ctttcagctt
     4501 tcttttcgtc tcttgatttt acttttttat aatctactaa aaactcatat aaattttcta
     4561 aaacactata aatttctttt aaatttatat ttttagtatc ctgagcaata acataatctc
     4621 taaatcctaa tatctcttta tcagttatgg tatctttatt ttctaaacac atcttaatat
     4681 agtttatttc tttcgttgct acaactagaa catctgattt actatttatg ctactttctt
     4741 ctaaaatatt ttttatatct tcatcttcaa attctttact atcctttttg tatctttcca
     4801 tgattaaact tattctttct aatgaaaata aaatattcat tatgtctata cctctaatac
     4861 tgatataaaa gtcatctttt ctattatcaa aaataaccat tttcaaattt ttagatatag
     4921 aatttattct ataattacag ctatctattt ttctcatact agatttaatt atagaactat
     4981 catcattaat agtatttttt aaaagactaa ttttttctaa taattcatca catatagata
     5041 tcagttcttt atctccgaca tttttcatcc tatttctatt cataacaaat tgagcaatca
     5101 ttattataac tgctccaaca gctaaagccg acaatctcat aggaaattca ctttttccta
     5161 caggtatact taccataaat atatacatta gaccaaaagg tacatatact gctctcttta
     5221 agtcataact aaatacatac cctattataa ataatgctat aaaatttaat aaaactccta
     5281 aatacatatt ttgtactgca aaaaatgata gtattcctaa gattatattt attgctaaat
     5341 acttcagtaa atttttaatt ggagaaatag ttaaatctct ttccaagagt gttagcatag
     5401 ctgttactat acctacacct ataagtgtat ttttacttcc aaataaatat ataaatatag
     5461 aaataaatgc tattataaaa ataaacaact ttgtttttga tattactagt ttctttgtca
     5521 tcatatcccc cctaaaaatt tcaacaagac caattttatc atatttgtta tcttattcaa
     5581 aatgcgcttt tttacggtag gactttaact ttttttatat ttagaactat ataattcata
     5641 tataaacgta ttttttttat atataaccga tatattttcg tatgctccca tatttttttt
     5701 gtttcaaata ttttttcaaa aatataaaaa taactctctc aataatacta aaaatgtatt
     5761 tatcaagaga gttataattt atacaataaa tttaattaag cttttcttta cctaatatat
     5821 aagttccaag acccataaat aaagctcctc caacaatatt tccaagtgtt acagctacta
     5881 aattataaat agctcctcca atagtaactg tacttattgt tggtgaaaat aatgaaacac
     5941 tataaattgt catatttgct acactatgct caaaacctga tgttataaaa gcaaataaac
     6001 ataagaatat cataattatt tttgcagtat cactattagt tctaaacgaa cacaacactg
     6061 atacacatac tagaatatta cataaaatcc ctctaaagaa aagagctgta aatggcatgg
     6121 atgctttact agctgctgta tttgcaaaaa attcagctac aggtcctttg tctactagac
     6181 cagttcctac aaatataaga cctaaaacca aagctccgat taaattacct acccaactgt
     6241 atgcccatat tttagaagta tcttttatac ttacaccttt atttaacata ccagcagaca
     6301 taaccatatt gtttccagta aataactctg ttcccgtcat tataaccaaa cttagtgcta
     6361 ttgcaaatga taaccccatt actatcttag tcataggtga acctgcatca gtcaaaagtc
     6421 ctcctatagt aaatatcaaa agtataccta tacctacata aagtcctgca aacgcagaag
     6481 ataccaaata ttttacttta cttgtgttta ataaatttat cttatttata gctgcattag
     6541 ttaatttatc caaagtttct ttatgcataa ttccctcttc cttctattaa attttaattt
     6601 attcaaatat atgttttact caatttatag atatttaatt ccactccagt atatgttttc
     6661 gtacatttta gttatttctg gaagctctac accttcaagt gcccattttt taaattccat
     6721 aaatcctgtt ctatcaatta tatatcctat atgttctttt cctcctggtg catctttatc
     6781 tatatacttt tctacatatc tataagtatt aagtattatc tttattatgc tgtcctcatc
     6841 tacccataaa agaaaatctt ctgctagtct agggtttttc tttcctgttc tacccattat
     6901 agctaatcta tagtattttt tctcatctct agtccatgca tttgttgggc aatttagtac
     6961 acactctcca catcctatac acttatcttt atctcttacc accttattat tttctgtttt
     7021 taatgctcca acagatattt tagaacattt ttttacacat gctccacaat ttacacatct
     7081 atctttttca tatattggta aagccatacc aataattcca aagtcattca ttctagcttt
     7141 tatacaatca tttggacaac cagttaaagc cactttaaaa tgtaaatcat ttggaaatat
     7201 agctttttct attcttttag caaattcagt tgtattatat tgagcctttg gacagacttt
     7261 atttcctata caagctgcta tatttcttgt acctgctgct ggataacctg cgtttttctc
     7321 actttgattt atattcattt tttcaataac aggttgtata attttattta tttcttccat
     7381 atcttccata tttattccta atatttcaaa cccctgtctt gtagttatgt gcacctgtcc
     7441 atttccgtac tcacttgcaa ttttagaaac tacagctaaa ctctcagggt caataagacc
     7501 accaggtact ctaactctta aagaagtctc atattttgtt tttgtaattc tataagcatt
     7561 tttcattact tttttggtat ttaaatctct tatcacaaac ttcgccccct aatcaataag
     7621 atttttagca tatgaataat caaatactgg tccatctaaa catatgtaag tatcatccat
     7681 tttacaatgt ccacatttac ctattccaca acacattttt ctttcataag aaacccaaat
     7741 gttcttttca tctaaatctc tttttaaaaa ttcttctact gtaaatttca tcatcattgg
     7801 aggtccaact acaactatag aagtattgtt tatatctttc atatccaatt ctggtatgta
     7861 ttttgttact aatcctacat taccactata tccttcctct gctccatcta cagtaactaa
     7921 aatatcaagt tttttactcc atctctttaa atcatctgca aataaaatat cttttggtga
     7981 tttaaatcct actattaatt taaacttttc gcatttttct ttattattat aaaaatattc
     8041 aactattcct cttacagggg ctaatgcact tcctcctgcc actactacaa tttctcttcc
     8101 ttcataaagg gatacatcaa atccatttcc ataaggacct cttataaaaa aagaatctcc
     8161 ttctttataa ttaaacaact cgctagttac ttttcctaca tttcttattg taaagtcaat
     8221 ataatcctct ccatatcctg atactgatat tggactttca ccgtatttag gtatagatat
     8281 ctcataaaac tttccaggca atacaccttt tgtattgcag tttactctaa aagtccattc
     8341 tttatcagta tgtttaacta tatctagtat ttttgcactt actggtatat atgggttcat
     8401 ttatacttcc tccttactaa ttactaaatc atttattttt tctatacact ttgaaaatga
     8461 tatgtattgc ggacaggcat catcacatct tccacaacca acacacatat gatatccgaa
     8521 tcttttttta aaatcatgta ttttatgcat agttttaaat ctcattcttt caccttgttt
     8581 ttttctaaaa gaatttccac ctgctatatc tgtatatcca tctacttgac aagaagccca
     8641 aacccttctt ctctcaccta cattttcgtt ttccttatag aaaatatcct gcattgtaaa
     8701 acatgaacat gttggacata caaaattaca tctaccacat cctatacatc ttgtatcata
     8761 ttcatcccat attgaatttc ctataatttc atttgaatca atattctctg gcacatctac
     8821 agatattaaa ttatcagtta cataatcaac attaaattca gttgcttctc cattaaatac
     8881 ttcaaattca ctatccttta tatctaagta cagtgtatct ccttctatat ttaatccaat
     8941 tgaataatta tcagttttgt tagaatccat gctcacacaa aaacagtttc taaaactttc
     9001 cttacaccct acaagtgcaa actttacttt atctctgata tttttataaa aataatcttt
     9061 ttcttcacca ttatttagat atatttcatc aagtcttttt ataccattta tgtcacaagc
     9121 tcttaagaaa attaaaattt tcttgttgtc taaatcgctt tccttaaact ctttttcagt
     9181 aaaataaaac aatacttgat ttatgggaag tacaacttct tttggtgaaa agtttgcttt
     9241 cttagcaaat tccatttctt caaaagcgtt tacctccttg tatcttataa catcagtatc
     9301 tgaaaaagta cctttaaatg ggattaaaac tggtgcaaat atcttgtagt ctttttttag
     9361 ataattcaga ccttcattga acttagatga atctaatttt agtttcattt ttatcacctt
     9421 tcttatattt tagttttact tacttgctat cttgtttaaa tcttaacact ttattttcta
     9481 tgcttctgtg ataattatca cagtttcaaa aataataaaa aaatattaat agagtttact
     9541 aatctctgtt aatatttttt tattatctat attattcgat ttcaaatcta atcacttaga
     9601 tacaatcatt aaatacaata aaactattat ctactcaatt ttttgaataa taaagaatct
     9661 tatttgcttt tttatacaat tcaatagggt aaaataaaaa ttaataaatt agacaataaa
     9721 aaagaatcta taaatagatt ctcttaataa tatttttatt taattaaaat tctaataata
     9781 cttttactta atcataatta attatttttg aaataccttg ataattcagt ttctttaaca
     9841 taaaaacctt tattatcaat cttaataagt tcttcttttt gcaatacctt tatcattctg
     9901 gaaacagtct cccttttaca gccaagcata tcagctaaat atgtaactgt aatatttaca
     9961 tttagtaaag tccattctcc acagcaaact ccaaattctc tacttaacct atataatttt
    10021 gctgcaagtt ttttatctac ttttatggat atagaatttt tcaactgtct atataaacgt
    10081 cttattcttc tttccatata agaaataata tcctttgtaa gttgaaaatc attttgcata
    10141 atttctaaaa attcttcaat actataataa gctacaattg aattttcaaa tgcttcacag
    10201 ccaacagtgg agtttttaga agagtcaaat gtaatatcat ttatgacttc cccactattt
    10261 aatatgaaaa ttacttttct ctctccattt tcacttatct taaaaataga tacctttcct
    10321 tctaaaagta tataaacact gtctaagcta tctttttcat aaaataaaat ttcttttttt
    10381 tgtaatcttt ttagtttagc tttttcttta agttgattct tcgttttagt acttaaatta
    10441 ttaaaaatta ttactttatc aatattataa tcttttaatt tagacatgta atatctctcc
    10501 attcctaaat ttttaagaga gctctataaa acattacaaa tttcttattt tattcatttt
    10561 taaacaattt tatagcattt gtagttttaa ttttcctttc aatagcttca ttagaaagtt
    10621 cttttactac ctgtgtatat actaaatcaa taatgaaaaa ttgtgctaat ttagaagata
    10681 ttgaaccagt ttccagtact gtttctcctg atacataaga taagtgtaca tctgaaatat
    10741 cctttaattc agaattttta ttttcagtaa ttgatataat tttagcatta ttggctcttg
    10801 caagctttac agtttttata atctcttctg tttcaccaga atgtgaaatt gcaattatta
    10861 aatctccttc ttccattatg gaagacatca ttatcatcgt atgactgcta tcaaaactta
    10921 cacaattaag gcctattctc ataaatttat agtttgaatc ctgtgctatt ataccagaat
    10981 atccaatgcc tatgaagtaa actttctttg catttattat aagccttgca cagtcatgta
    11041 catccttact attgattatc tctactgtgt tctctaaagt ggttacattt gaacttaaaa
    11101 gtttttttgc tgtatccaaa gctggctcat cattttgtat atttttattt ataatatttt
    11161 ttttattgat agttgatatt tcttgtgcta aagtaacttt aaaatcctgt aatccattaa
    11221 aattcatttt ttttacaaac ctagttatag ttgcttcacc aatattgctc tctttagcta
    11281 tttgtgatat tggtttataa aatacatcat ctatattttc ttttatataa gctattaata
    11341 atttatctga ttttgtgact ttaaaagttg gtgattccaa ttgttctaaa atactcatta
    11401 aaaacccttc tattctttat attttgttta aaaaattata taatgctcca agcattcctg
    11461 catcattttg tctctttgca aattctatat tagtgttttt gtaaactctc tctataagaa
    11521 cctcctttaa tgctttgtct atttttggtt ttaggaactt ttcttgtgcc ataatcccac
    11581 cacctaaaat taccacttct ggatttatta cataacatat atttgcgatt ccaactgcta
    11641 atgattttat catattatcg agttctttta aacaatcttc atcattattc tttgctaaat
    11701 caaaaattat ttttccattt aagtcctttt cattcaaatt ttttattttt gctacttttt
    11761 taattagact gctagtagat gccaattctt gaaaagaact tccatttata ttcatatatc
    11821 ctacttcacc agcgctatta ctaaacccat ttatcagttt attgtttatt ataatacatc
    11881 caccaattcc agtacctatt gttaaacata tagaagatgt tgcacctcta cctgcgccaa
    11941 gccacacttc accaagtcca gcacaattaa catcattttc aacttcacat tttatattaa
    12001 actctttttc tacttctttt tttaattgca tacccttata atttggaatt aattcttcta
    12061 atgcaaatag tattttacct tcaactgggt caaccatacc tgcagttgat atacatatac
    12121 cacttatttc attttcctct atatattttt ttactatatc ttttattttg gctaatattc
    12181 cttctccacc tttttccttt gcttcagtat caagacttga tttatcaatt ataaatccat
    12241 cttctctaat aaaaccatat tttattgaag tacctccaac atctatacaa acgtaatttt
    12301 tcataacata tctccataca aattattttt ataaattaac aactctattt aaaattttat
    12361 attacacaaa tcttttactt atcttttcca attcacagat atatttcctt gcttctgata
    12421 agtctatagt atcacttaat acactttttt ctttggttat atgatactta taaatatttt
    12481 tacataagtc ctctcctaac atattaagac cttcttgagt gtaatgaaca tcatcaatag
    12541 ttaatcctct gtcatgaaat tgcatagcaa ttttacttcc tacacacagt atatcacatt
    12601 cattacaagc agattcttga gctgctgcaa ttcgtcttgt tctaataaaa tattcatctt
    12661 ctccaagcca ataaccagtt aaacttacaa acattttatc aatcatacat tcttctttaa
    12721 gcctatacgc aatttttttt agactttctt tatagtacaa tgcatcacta ccatattttg
    12781 catcactttc accttgaatc caaataacgt atttattttc tatattataa tgtttactta
    12841 aggtagtaca gcctgtatta aatttttcta tagtgtcatt tagaaaccag ttattatcat
    12901 gattccagtt ttttattctt gaaccatctt ttgcattgtg aataaaacaa actttatttc
    12961 cagtaagctt attccattca tttgaaaatg caggtgctat agtaccatca ccttctgaag
    13021 tctctagctc acatctcata tttataattt catcagtact caaatattca aaacaatttt
    13081 catttggaat gatgctctct tttggatttc ccaatcctct agcattgctt tgtcctatta
    13141 ataagaatac atctatattt tccataagtt aactccttta caaaatttta agcttttaat
    13201 tattttcaat tttctcataa aaaactaagg ctgaaaatag taatctaact aaaatttacg
    13261 aagtctcact aatcgctgaa agttaaatca ctattttcag ccctagtggt tatataaatt
    13321 atcattatct aaccaaaacc ttaaaaatag cttttttgat attttctttt tctaaaactt
    13381 ttattcctgt catatgagca tcttctggat aacaaactaa aaaatcacct tttctaagtt
    13441 ccatgtatcc attattttta ccatcaagag gcaagaaatc gcccttttct tcatatattt
    13501 tttgttctaa attttctata aaatttatat ctattcgttc acatccatca agcatcaaat
    13561 gaacatctat atacttctta tgagcttccc agaatctatc ttctttttca caagttttat
    13621 actctacaat attcacaaaa atatcatcac catctattgt ataagtacct ttttcataat
    13681 ctattaatga attatcttta gcatattcaa aacatctaag taaatcttca tgcaatgttg
    13741 aatatgtctt actatgattt atatttccaa atatcataag cactatctcc ctatataatt
    13801 ataaagtcta tttttatact tttatataca aagtatatta tacaatctta tagagtaact
    13861 aaattattta tctaatcact ctataagaca atttatcatt taaatattat ttttcataat
    13921 aaacagtagt ctcagctttt ggtaaagata cttttgcttt aactttagct tctattgcac
    13981 taactattgt acctaccact acagaagtcg tgattgttat tagagaatat gcccaagaag
    14041 ttacttctgg catacggtat tttaaataaa caactaaaat tgctgatact atgaatccaa
    14101 catatgctcc cattttagtc gcttttttac aaaatgcacc aagtacaaat attcctgcta
    14161 aaacacctag tactagcccc ataaaactat taaaccattc atatgctgac tttatttctc
    14221 catttgctaa tactattgca acagctatag aaagtatacc tacacctaat gatatatatt
    14281 gtgctatctt agtttgcttt tccatactta tatcttttgt tatgatagtt tgtatatcta
    14341 aagtccaact tgtagcaact gaattaagcc ctgtcgataa agttgattga gaagctgcat
    14401 atatcgctgc aagtagtatt cctgttatac ccactggtaa ttcataagct atatatgatg
    14461 caaatacttg gtcttgttga actgtttgag ctaaatctgg gttttgagta tagtatataa
    14521 ataaacaagt tccaattaga taaaatactg ttgcaagtcc cattgataaa acaccatttc
    14581 catatgtcat tttattaagt tgctttatat cagttgttgt agtaaatctt tgaactatat
    14641 cttgactcga tacatatgat gaaaaagtat taagaccagc tccaataaat attataaatg
    14701 cacttgtaga tagtatattt ttatcaaaca taacttcatt acttgctaag aacttgtgtc
    14761 cagttgtaag agtatccatt actgttccaa aaccaccatt tatacttgct attaaataga
    14821 acaatgtccc tgtaacacct actattaata ccataccttg tataaaatct gtccacaaga
    14881 cagcctttaa tccacctgta tatgaataaa ttattgctat tacacccata actactatca
    14941 atacatttac aggtattcct gtaagaccag ctagaaccac tgatggaaga tacattatga
    15001 tagacatacg tcctatttga tatattataa acatcaaagc tccaagtact ctaagacctt
    15061 tactttcgta tctcatttct aagtattcat atgctgtatc tatttctaac ctactataca
    15121 gaggtaagaa aaattttatt gtcaaaggta ttgctataaa catacctaat tgtgcaaacc
    15181 acaaaatcca agtaccacca taagaatttc ctgctaatga taaaaatgat attggactta
    15241 ataatgtggc aaatattgat actgatgtaa cccaccacgg aatagttcca tcacctttga
    15301 agaactcttt tcccttcatt tccttttttg aaaataacaa tcctgcaaat aaaactgcca
    15361 ataagtaaac cactagtatt gcaaaatcta tccaagtaaa tcctaccatt tgactaatcc
    15421 tcccatacta aaagtatttt attaaaaata ctttctgtat atttcttttg ctcttgattt
    15481 catttcatca gttgcttctt tcataggttg tctacagtat ccagcctcaa caccttgttc
    15541 ttcaagtagt aatttaattg tttgatataa gccatttcct aatatatctg ttattaaatc
    15601 attagtcaca tgttgtactt caagtgcttc tgatattttt tcattcttag ttaattcgaa
    15661 tatttgtcta gctctgacac cgtttacatt gaatgtagaa ccaattgccc catctacacc
    15721 taaaactgta gctggtaaca tcatttcatc aaatcctgca aatattaatt tatttgggaa
    15781 tgtttttctc attctctcta ataagtagaa atctgctgct gtaaacttaa cacctattat
    15841 tttttcattt tcaaataatt ctccaaactg gtctaaactc atatcaacac cagttaaaaa
    15901 tggtatagag tatattatta atctattatc tacagaattt attattgtat tataatagtg
    15961 ttttatttct tcaaaatcaa atttatagta aaaaggtgta acagcactta ttgcatcata
    16021 tcctaaatca gttgtaaatt tagcaagttc tacagcttct tttaaattaa ctgaaccaac
    16081 ttgagctatt aattttattt cttctttcac ttcatcttta gctatttcaa atattctttt
    16141 cttttcatca gttgaaagca tgaagttttc ccctgtactt cctcctacat aaagaccatc
    16201 tactttacaa acatctatat tgtgtctaat tatttgtctt aacccttttt catttatatt
    16261 cccttcctta tcaaatgaaa ctaataatgc tgaataaatt cccttcatat ctgttgttct
    16321 catatcttta cctccaattt taattttcaa gtatagttaa atataataat tttttaattt
    16381 ataaattaat ttttaattaa tttcaattgc tttaacaaat ttttctgtta ttaattgtgg
    16441 tcttgttatt gctgaaccta ctactgatga atgaacaccc aactcaaaga ccttttttaa
    16501 atcttgagga gtatttacct ttccttcagc tattacaggt atttctaaat ctttaactaa
    16561 tctttccatt aatacaaaat ctggaccttc taatgtttta gtatatggag tatatccaga
    16621 tagtgtagtt gaaacgcaat ctacaccgta ctcttgtgct ttgatagcct catcataatt
    16681 tgaaatgtct gccattacta gaacaccgtt ttcttttata tactttataa gttcttttaa
    16741 gtcttcatta tttggtctaa ctctattggt agcatctaat gctatcattt cacaccctgt
    16801 tgttaaaagt tcatcaactt cttttttagt tggcgttata tatatatctg aatcttcata
    16861 gtttctcttt ataataccta taacaggaag tccagtaact ttttttattt caataatatc
    16921 ttcaactcct tgagctctaa ttccaacagc tccaccttcc atagcagcct tagccattct
    16981 ccccattata aaaggactat gaagaggttc attttctaaa gcttggcatg atactattaa
    17041 tcttcccttt accttatcta gcatttttta aattacctcg tttctgaaat ttattttctt
    17101 gatttatgta ttaataataa catgaaaact ttttctttgc aatatttttt catatttttt
    17161 tctcgcatct atatttttac tgatttattt tatatattag cttgtttttt acatttaaaa
    17221 gaaattttat ttcatttttt tggtgtttat tttcatttta tataactact gttataagtc
    17281 catttaaata atttcaataa aaaaacccat gtatactata cataggtttc ccaatattat
    17341 tctaaatcca ttataatttt tcttccacag catggacaaa attcagcctc tgtatctatc
    17401 tcactttcac aataaccaca aattataaaa tcatcacatt cattttccaa ttcaaatttt
    17461 ttaccacatt cagcacaata tttatcattt acatttccaa cataaccaca ctcacaagtt
    17521 acattacttt ctgccatttc catttcatta atttccatat ttttttcata aattaactta
    17581 tcaagttcta gtaactcatc acagaattta tcaaaactat tatcattaat agaatcttct
    17641 ctaatttttt gatatgtcag tattcccatt tccaagaata tctttgcttt cttttcttga
    17701 atatcatcaa tctctctatt taattttgat ttttctttat ataattcaac tttaaatttt
    17761 ccactaccaa actttttctt aaccttagaa aatttatcat ctactttatt attagtattt
    17821 ccttccacag aaattccctc caatcatatt taatcagctg aatttagctt atatcctatt
    17881 aaagatatca caaaactata tacaaatgaa attacaatag cttgtaagac tttaaattgc
    17941 attattaaag ttgttccata attagttgta ttaaagaaat taatattact atctagtata
    18001 aatgtactaa ataaagccaa tattcccatc aaaaatgcat aatatatact aaatactatt
    18061 ataggtctta tactatctgt attatactta aatcttagct tgtatccatt taataataat
    18121 attaacatag acaatgctcc cattgcataa aacattaatt tagtatctcc aaataaagat
    18181 gaagaactta aagtaaacat tgatactgtt gagtttatta tggttactga aattccattt
    18241 gcaaaagccc acatataact agctatttgt gggagtatag taaataaatt taatccattt
    18301 gaataactac tcatatctag ttcatttaag taactactat cagatatagt tagtaccaat
    18361 agtattacta atactaaaac ataacctaat atgaatgttc ttatagaatt tgaaaaaaga
    18421 gataaataca tattttcctt ttcataactt tttttataag tagttatgta tgtacatata
    18481 aaccctaata caaatccatt taaaagaaca ctaaaaaatt cataactata ttctaatgca
    18541 tatccatatt ctaacatact atgtgaacta gtttttacat tagtaaaaat agataaaata
    18601 gctaaaacta atccataaaa aattccaact cctagagaat ttgctaatac agtttttgaa
    18661 tctctacatc tttttctcat aaaaattagg tttgatatta ataataccaa aatagggatt
    18721 ataagtaata tcaacaaacc tatattggca tttataaacc cagaacccat catggtagac
    18781 atagatacac taatttgacc caagttgaga gctaggatta tatgtgctgg atttattaaa
    18841 taactaatat cattaaattg aatagttata atagctttaa atatcaatgc aataataaac
    18901 aatataaata tggttgtaaa tgttgttaaa aatacacctc ttttatttgc atgatatgaa
    18961 agctctttta ttttagattt caagtctagt tttgtttcaa gtggtcttaa tgatgctatt
    19021 tcataaagtt catttccaca aaatttacag taattatcct tagtattatt ttctttattg
    19081 caactagaac aaattttttc ttctttactt ttaagagtga tatggctatt ttttattttt
    19141 ctagttggtt cacctgtctt tttggaataa ttattatgtg aataattcat attatttatt
    19201 ttcttttcac ccattttttc cctccaagtt ttactattgt aaattatcaa atataactat
    19261 atcatataaa acttaattat accttaattg gatatattta tctattttat ttgtttattg
    19321 ctgttgtttt ttaacaggtc ttgatattta aatagtttgt taatgcgaac tttattattt
    19381 atattttatt atgcttaatt taaatttatt ctattcttta ttttcagcag caaactatct
    19441 gtaaatctta attatagtaa agtattaatt aataaagtta ctcaacatta gctaataaat
    19501 tttatctatc acaattttag attgttacac acaatatatt ttgatataat taaatcataa
    19561 acgaagtatt ccaaatatct aatgatagaa aggaatactt atttttatgc tttattttta
    19621 atggaggctt atatgaactt agaaaaaata aaaaaatttt ttggatttcc tctagacaaa
    19681 aaacacaaag aaaatgttga aaaagaactc tgcaattatc tttatcatac gggtaaaatc
    19741 atttgcataa taatagtaat atctcaaata attatgatga tatcaatctc agctagagaa
    19801 ggaggccctt ttgccacacc aagacgccaa tggtattttt atatgtatgt aacactattt
    19861 tgtgtatctt ctgtctttat gtttgtattt tcttatcttt ggaataaaaa aaaggattgt
    19921 tgtacgtttt atataaattt atggataatt tatgtgactt ttttctgcct atggggttgt
    19981 gtaattactc tacttgacca gttaggtgga aatgacctaa gtgtatttag ttatattatg
    20041 ttagcagctg cagctttctc agtattaaaa tttggagaat cagtaatagt ttttggagga
    20101 tgctttttgt ttcttaattt attcttacct gggattcaaa ctataccagg caatgagttt
    20161 agtaatattg tcaacacatt ttttataaca tcccttgcca tttttgtttc tacaatgcta
    20221 tatagaaatc gtgtatttac ttactatgat aaacttttaa ttagtcaaca atatgaagaa
    20281 atatctcata taaataattt acttaacgac aaagttatga cagacgacct tactaaaatg
    20341 aacaaccgta gataccttga agatattcta caaaaaaaat taatcagcca tgcaaatcaa
    20401 gaacctataa ctggaatgat gatagatata gattatttca aacaatttaa tgataattat
    20461 ggacatcaaa agggagacta ttgtctacaa aatattgcca aagtaatttt agattttaca
    20521 agttctgaaa atgaacatgc tatacgatat ggtggtgagg aattttttat atgtattttt
    20581 aactgtgatg agacagatgc taaacttaaa tcagaatgtt tacgaaaaat tatagagtcc
    20641 tgcaattttg agcgtgatga tgttccttta aaatgtgtaa ctgttagtat tggagtttat
    20701 acaaagaaaa ttgaaaaggc aataacatta aatgagttta taagtaattg tgataaggct
    20761 ctatatactg caaaagaaca gggacgtaat tgtgttgcat tttattaaaa agttacatac
    20821 tattatataa acctataagt attatatcgc aaaataaaag taatcaaatt atatatttat
    20881 agtacaataa aaataacttg catatctcta aaatagataa atatgcaagt cattttagtg
    20941 tttatactta aagttatata agttttatta tatataatta ttaaaattca gcatttttag
    21001 gagttcttgg gaaaggtata acgtctctta tgttagacat acctgtcata tacataatta
    21061 ttctttcaaa tccaagacca aatccagagt gagtagcagt accaaacttt ctaagttcta
    21121 aataccacca ataatcttct tctcttaatc ccatttcatt aattctatct aacaatacat
    21181 cttctctttc ttctctttga gaacctccta cgatttctcc aactccagga actaataaat
    21241 ccattgctct aacagtttta ccgtcttcat tcattctcat atagaaagct tttatatctt
    21301 ttggataatc tgtaacaaac actggacaat tatatatttg ctccgttata tatctttcat
    21361 gctcagtttg aaggtcacat ccccactcaa caggatactc aaattcatga ccagattcaa
    21421 ttaataattc cactgcttta gtatacgtta ttctaataaa ctcagagttt actatcttat
    21481 ttaatctttc taacaatcct ttatctataa aactattaaa aaattccatt tcttcaggag
    21541 cattttctaa tacatagctt ataacatatt ttatcatatc ttctgcaagt tccatgttat
    21601 cttcaaggtc tgcaaaaact atttctggct ctatcatcca aaattctgaa gcatgtcttc
    21661 ctgtataaga gttttcagct ctaaatgtag gtccaaaagt ataaacgttt ctaaaagaaa
    21721 gtgccattat ctcggcattt agttgaccac taactgttaa attagcctct tttccaaaga
    21781 aatcttgagt atagtctatt ttaccatctt cagtttttgg aatatcattt aaatccatag
    21841 ttgttattct aaacatttct cctgcacctt cacagtcact acccgttatg attggagaat
    21901 gtgcatatac aaagtttctt tcttggaaga atttgtgtat tgcataggca gctattgatc
    21961 ttactctaaa tacagctgaa aaagtattac ttcttggtct taaatgtgct atcgttctta
    22021 aatattcaag cgtatgtctc ttcttttgta gtggatacga actatctgat tccccttcta
    22081 caactatgct ttttgcatgt atttcgactg gttgtttagc cccttctgta gatactaact
    22141 ctccctctac aagtatagat gagcttattg ctaattttcc tacctcttta aaattttcta
    22201 ctttctcatc aaatactatc tgcatatttt taaagaagct tccatcattt agctctataa
    22261 atccaaaatt tttagatact ctagatgttc ttatccatcc agctactttt acagtcttcc
    22321 ctatgtactg ttctttttct ctgtacaatt cttttactgt tataaattcc ttttgcattt
    22381 taaatcctcc tagtataatt gttattaatt tcttttaaat ataatttcaa taaaaaaagc
    22441 ccttcatccc tatataaagg gacgaaagac tattactctc gcggtaccac cctagttgtc
    22501 ataatgacct cttagttaag caatgcttaa agcctataac ggtgccttcc gtctaagtct
    22561 acttactaaa taagctttcg attagaaact ccaagatgtt cttcaatata aacctcgtat
    22621 tgagctttca ccatcctcaa ctcgctagaa ctacatttta tatctactct tctcttcaca
    22681 gtatttatct tttattattt ctactaatta acaaaattat accaatttat aaatttttag
    22741 tcaataaatt tatataattg atactgattg ttatttaaaa tataataatt ctaccttgca
    22801 taatgataat aaatacttat cttcattact tctttattac ttaaatttcc aaaattttat
    22861 aatttacttc cattgtcaac ccagtctttt gcttcttcga cacccttaat tgaaggtatt
    22921 gaaactttac tatgaggttt taatttttca acatcagccc atgctgctgt cgactcattt
    22981 ccaactaaaa gtgaactttt aagtctttta ttatctttac cttttaatgc tcttgccttt
    23041 ttactttcca aaactatcac ctctattatt tattttgtaa tagttttagc aattttacaa
    23101 ataaaattca taagtctaaa ttataatgat ttttaaatta acatcatatc ttttcaatac
    23161 ttaatttata cctatttata tacaaaatat ttactttgtg tattcaaaat ataaaactgt
    23221 ttttttaatt ttcatattta ttttttatgc catttttcta ctctatctaa agtatttgat
    23281 acaattaaat tactaatcaa ttataatttt acataggttc ttatctatag agtatttgct
    23341 atctgttgaa tcgtattttt taaatttaaa atcccatatc cctgcgatac atttggatat
    23401 gtatatatct caagttttgt agcacccaat attaaatatg tttttaaaac ttgtgtaaac
    23461 aatgataatc tcggtacatt atcctgtttt tctaagtatt ccatcaataa ggctaaaaca
    23521 cctgtgacta tagaactgct cactcctgtt cctgttcctg tattatatgt tccattttta
    23581 tatgtggata ttatatctac acctgaagct actatatctg gcttaattcc tcttcctcta
    23641 attggtcctt tcgaagaacc aatccacata ctatcagtct tattattaaa tgtaccaact
    23701 gtaattacat catcactagc agcatacata gttatagtag ctactgaatc agggtctaaa
    23761 aaccttgtgt ctggagctat caaattttta tttggtaagt atatgtcata ttctccactt
    23821 attataagtt ctgaagtcaa tcttaaaatc catactccag gttttatatc tcttaatctt
    23881 atctccaaat tttcttttcc agaggtaata tatggataaa taaatctcat tgcataagta
    23941 gtattttcta agttaaattt tcctctatat atataaaagt ctggagaata ccttatatca
    24001 tgactaactt cacctgaagg agatattatc tgtgctccca ctttatctgg accattcgta
    24061 tttagtgtta tatcaagagc atagtcatct ccatcttgta ttattacatc ttgaacttct
    24121 cccacactgc taaatctgcc agaataatga atatctgtat tcccttgatt tcctgcacca
    24181 ctcactacta ccacaccagc actactcaat atattaaaag tatctaatat tgaagtagtt
    24241 gctacagcac ttgacttaac tccaatagtc aaatttatta ttagtggttt attttctgtt
    24301 cttgctatat tagtaacata cgttatagct gctaaaaaat ctgaaacact ataatttatc
    24361 cttccagcat aataagtatc tgtatatgat tttaatttaa ctactattaa gtctgattct
    24421 gtagtaatac ctctatactg cgaatttatc cttccatttc cagctaatat accagaaact
    24481 aaagttccag tacctatatt atcttgactc aaacttccat catttctatt tatggctata
    24541 tttaactgac ttcttgtaaa ttcacttcca aaaataaaac cttctggagg tggatttgta
    24601 tttgcttctt ggtcccataa atatagaact ttactagttc catcatcatt tataaaatca
    24661 ggatgcaaat aatctatacc agaatctata actgctaata aaattcctct tcctgttata
    24721 tcattatatg gattttcata aatataatca gtttcagctg ctgttgtaat ggtttctcca
    24781 ttatttacat tattagttat ttcaattaga ctgctcattg gttcagattc ttcccaccaa
    24841 gcaacttgta ttatattatt taaaatagtt tcatcaaaat ctacaggtac atatattact
    24901 gcaagctgac tatttaaaac catataccta tttattccat tttcttgtag ggcagattct
    24961 atatcacctt gataaattat acaataagat ttttccataa atccctccta tcttaaaaca
    25021 tcaaacattc ctctaacatc caaaagccca tatccagaat ttgtatttgg atatactgtg
    25081 tttgaatctt ttctagcacc agcttgcata aaagttttta ttttttgcac atatgcttgg
    25141 tttggatatc taccatcaac aaaagtatat tggaaataca ttgcagcagc accagctgca
    25201 tgtgcacttg ctgctgctgt accagttata gttgcatacg tgttcccagg ataagcagct
    25261 attatattga cacctggtgc tactatatct ggttttaacc tgtcttcaat agttggtcct
    25321 cttgatgaac tttgccataa actaccatta atagtattgt aggctcctac agttattaaa
    25381 tcatcttgaa tagcaggata atttatagtg taaaaagggt ctacctctct aaatctagtt
    25441 ccacttttta aaagttctct atttggaagg tataagttat atcttcctgt tataatataa
    25501 acccctacta atcgaatctt ccatacacct ctttttgcat tctttaaagt aacattagta
    25561 aattgctgcc cagaaaaagt tgttgggtat atatacgtta tagaatattc agtcccctct
    25621 aaatcaaata aacctgttac tttattataa tttgaaattc caacactttt actttcttca
    25681 ccagtgggag atactattat tacatcagct ttatctggtc tatttaacca aagttctaat
    25741 gataattctt cctcgtcttc atttaactct aattcaactt caacagaacc tccaacatgt
    25801 ggtattattc cagatgtatg tgtctgtgta ttgccctcat ttcctgctcc tgctgttata
    25861 cataaccctc tagtaaagaa tgctttttca ctattgctcc tattggttaa acctgccaag
    25921 ctactggtac ctaaagacat atttataact agtggtcttc taagttcaaa agctttttta
    25981 tatgcatact gagatgcagc aaacaacatt gcactattat aaaaaccatc tatcttaccg
    26041 agttttatta ttattagctc agaatcttct gctatacctg catattcact atttacattt
    26101 ccaagccctg agcatatacc actcaacata gttccttgac caacttcatc ctgagataga
    26161 ctagggtcat tttctgcgat agctcgattt atatcttctc gtgtatattc tgtcccaata
    26221 taaaaaccat caggtggagt tccttctttt gtttggtccc ataaatatac tatttttgaa
    26281 gtaccatctg gataaataaa atctggatgt agatagtcta ttcctgtatc tgcaattgat
    26341 attagagtcc ctcttcctgt tatattaata ttaggattat ttttaaagaa atttacgccc
    26401 atttcttctg ttgcaactac acctccaaaa gtacctcgat taatttctcc aagtaatgtc
    26461 atctttgtag tcgatacagt tcttatgatt gaaggaagtt gcaaaactcg ttgtatacta
    26521 ctatagtctg tattatttat agatagtaca cctaatgaat cacttatctt aaaaaaattt
    26581 tgactcatac ctaacccagc tagttcttct tcaaattcgg gagtatggac tacatctatt
    26641 cctgatagta ttcctgattg tctacttgca tttttactta tataaatttc attctctaag
    26701 tcattatgta tttgtatatt ataaccatct ataaacttat acctattttc ctcatcttta
    26761 aatgacttat taatacttct atatccaata tcctccgttt ctaaatcttg atttatacta
    26821 gatagagtgt acaaatctat atctgataaa tttaaaaaac caaatcctga tgaattattt
    26881 ggataagatt gattacttaa tctccttgct ccctttaaaa gtaatgccct taatttctgt
    26941 gaatacaaaa ataaatcatt tccatttaca attccccatt ccataaacaa tgaacaaacc
    27001 cctgtaacat gaggtgtagc catgctagtt ccagttaatg ctccacttgt acctcctggt
    27061 aaaaaagata ttatatcttc acctggagct aataaatcag gtttaaaaac acctaattgt
    27121 gtatctcctt caccagaaaa tatagatact atgtctgttc ttgaattaaa actacctact
    27181 gttacaactc tacttgctgt tccaggaact gttacagtaa gctcttgagt aggaatcaga
    27241 aatctagtat ttctatttag tccttcagaa gttggcaaat atatatttac attaccagtt
    27301 actatatcta ttggttcaaa tacaattttc cataacccag gagttatctg tgtatttgaa
    27361 cttaattgta gagtaactct tcttgtaaga gagtacggtg ctatagggta aaaataacca
    27421 gttattctag tttcacccaa agtatttctt atctcacctg aagttagaga gattgcttgt
    27481 gtttgattgt ttgatgggtt taccaaatgt acactaaaat catcaacaaa atctggccat
    27541 atatttatgt ttaatatacg ctctccttcc ccaactataa actcaacttc ttctgtaata
    27601 ttattttgaa gcctaatcct tttatgacct cctttatctg cattatttcc agctgctaca
    27661 actatattgt ttttccaaaa taaacacata tcatctatat attgttcaaa taaagatgtt
    27721 cctctatgtg aaccttcatt acttccataa cttatattta atgtaacagg cattcttaat
    27781 tctaatgccc tatctaaaat aaatttaatt gccctcataa actctgtact tctcgaaaaa
    27841 atatcggtct gtatatttcc aactctaacc actattattc tggcatcact tgcaatagtt
    27901 gcacaaatac ctgcaacatg tgtcccatgt aaactagttg ttgaaatagg tatatacata
    27961 cttccatcta ttgcattatt tatatcttca ttagtatata atgttccttc tctaaaacct
    28021 tctggtggat ttccttgaat agattggtcc caataatata atatcttaga cctcccatca
    28081 ctatctctaa acacaggtag agtataatct attcccgaat caattatacc aattatagtt
    28141 ccctttccag ttaaaccagt tctattttta aaccccgtta ttcctgtact tgaaaaactt
    28201 tgcacatcct gagtctgtaa tataaaaggc ttttctataa attcaatttc tggataagtt
    28261 aaaagtgtat ttacatcttc ttcattactt gatgttatta ttgcatatga tgaattaagt
    28321 atctctactg atacacctaa ttcttcttct aatctcaata tatctccatt gtattttaca
    28381 attaattcat aatttattat aatcacctcg acttctaaaa ttattattaa tgtatatgtt
    28441 ttgtgtaagt ctaaaatgta ttagactata taatttttta agtttcagtt atacaattta
    28501 taatagatat attaaaaaat aaataatgat ggtacaattt tgaataaatc ataccatcat
    28561 ttatatttac tttatcttga aagcattaaa tttttcaatg ctttttcaag accttttaca
    28621 gtcagtggaa acatattaaa ttcactttgt atcattcttt gagtttggta ccaataatta
    28681 attgaatccc atccatctct aagttctgga ttcatccata caattttact gtactttctc
    28741 ttaaaccttt taagccattc tataccaggt gtgtaattat atggaccacg atagttacca
    28801 ccaatatgaa gtaactcaga aggcgacata gaagcatctc caacaattat aactttatag
    28861 tttttatcta tattttttaa tatccattca gtattaatag atttacttaa ccaacattca
    28921 ggtgttgtaa ataatctatc ataaatacag ttatggaaat aatatatctt tacatcttta
    28981 aaatgatttg atttacttac agactgaaat aatgtattac ataaagtgct ataccccctc
    29041 attgaaccac ctgaatccat aagcaataaa agcttcactg tattttttct tggcttctca
    29101 aaaaataact ttaaatatcc tgcattatta caagtctctt caatagtctt atccaaatca
    29161 agctctgtct ttggagcatc tattctagta gaaaattgac gaagtctctt tagtgcaact
    29221 tgaaattgac gcatatttaa aacttcatca tctctaaaat ctctgtattt tctttctcct
    29281 gcaacttcaa gaacagctct accataagta gaatttccac ctatacgtat accagtttgc
    29341 ccttttcctg aatgtcctaa ctcggaagtg cctcctgtgc ctatccaata attaccacca
    29401 ttatgaggag agtcttgttc ttttattcgt tcctccaatt tagctcttaa tttatcaaga
    29461 tttatatttg gattttgttc caatctttca aactcttcta aatctatatc tggtttttta
    29521 agccaattca taatctcttc tggaatttct tcttggtgtt ctataccttt aaaatactcc
    29581 aaaaacactg catcaaactt atcaaaatca ctttcacttt ttattaaaat catcctgcaa
    29641 agatagtaaa aatttgaaaa gtttgaatag cacagccctt tatccaatgc ttcaattaaa
    29701 gtcatccatt catttaaaga tatatttaat cctcttgacc tcaataaata aaaaaattca
    29761 ataaacataa ttatccagct cctattaact ataagtgtct ttgcatagat tcaatatcct
    29821 cattattctt aagtagtatt cctgcaaaag gaacttcttt ttctattttt tcaataggca
    29881 ttccactcaa agttaatgct tgtatccagt ctattaactc tgatgtacta ggcttttttt
    29941 gtatgtcctt taaacttcta atccaataaa aagtagtcat tacttgttcc aataggtgtt
    30001 cttctacttt atcaaaatgt actttaacta tttcttccat catgtctcta tcaggaaatt
    30061 ctatataatg aaatatacat cttcttaaaa aagcatctgg aagttctttt tccgcatttg
    30121 aagttataat aactattggt ctttgtttag ctcttacagt ttcttttgtc tcatttatgt
    30181 aaaactccat tttatccaat tcccaaagca agtcatttgg aaattctaaa tccgccttat
    30241 ctatttcatc tattaaaaga attacttgtt gatttgagct aaaggcctct cctagtttac
    30301 catattttat gtacttgcta atatcatcta caccttctcc tccaaattgg ctatcataaa
    30361 gtctttgaac agtatcatat acataaagcc cttcttgtgc ttttgtagtt gattttatat
    30421 tccaaattac taaatctttt tttaattcat ttgaaattgc ttgtgctagc atagttttac
    30481 ctgttccagg ttcgccctta atcaaaagtg gcttttcaag agcaatagca acattaactg
    30541 atgccatcaa ttctggtgat actacataat cactacttcc tctaaagtta gatatctctt
    30601 tttttatcat aaatcctact ccttttaaac agtattttct aaatgtatat agactttatt
    30661 actatttttg atataaaaat aataatacaa tacatttgta tagtattatt ctaaaattct
    30721 atatctaaat aaaatcacct ctaaaatatt taatttttat ctttatttgt cataattaaa
    30781 tgaaatatat attttaaaga ataataaaag atagcttatt gtacaaaatt taaattataa
    30841 aaacttagtt taatcaaagg caccactttt tttcatttta gattatatat aaacataata
    30901 taaataattt ttattatttt agctataagc ataaagtttt aacgtattta taatttcata
    30961 ttaatcatac tatattaata ctgcttttta ttgtataata agctataaat attaacttta
    31021 acttagattt aagtatatct aaatttaata aatataagaa aggttgtgtt attttgcaag
    31081 ataaaaaaat ttatctaaat attgtactca ctgtagttgt ttcatatatc ttgattaaag
    31141 ttattgataa ttacaaatat ttttttggag tgataagttt attaatgtca ctactaacac
    31201 catttataat agcatttgta ttagcatata tattcaaccc actagtaaag ttcttagaaa
    31261 gtaaattaaa ttttaagaga atatattcgt tacttcttac ttatggtgta ctcatagtta
    31321 tactaatttc ctttatatta tttacagttc catccatagt aaatagttta gctgatttag
    31381 ttgctcaaat tcctacatac atagataaaa cagagcagtt cttattcgac ttaggtaaga
    31441 gtttacaatc agttgaccct aatacattaa aagagtatgg tgacaaaatt atgagtgtaa
    31501 tgcctaaatt tagtaacttg cttattggtt cacttggtgg gatatttagc actacatttt
    31561 cagttggaaa atttatagta caatttcttt taggttttat aatttgtttc tatatattat
    31621 tagaaaaaga aaaattctta ctgtttacaa aaaaagtagt ttatatttcc ctaggtaaaa
    31681 aatatggaga ctttataata gaactatgtc aaagcttaaa tttaaatatt ggtaaatact
    31741 ttacaggtaa gattttagat tcttttatag ttggagtact ttctggaatt ggtctttact
    31801 ttttaaaatc tgaatatgca ctattatttg gtacacttat gggtgtcatg aatatggttc
    31861 catattttgg tccagtaata ggtatggcac cagttgtaat aattaattta ttctccaatc
    31921 caacaattgc tttaacttct ttaatttact taataataat tcaacaagtt gaagttacat
    31981 ttatagagcc aaaaatcgta ggtggacaac taggtcttag cccattcttt acaatcctag
    32041 cagttactgt tggtggagga ttctttggta tacctggtat gattctatct gctcctgtga
    32101 tgggcgttat aaagatatac ttctgtgagt ttgtaaatcg tagacatgat aaaatacaaa
    32161 tggaataaaa atacaattta attacgattt tgtaatcaaa agttacagct gtgtaactgt
    32221 aatattattc tctttaatgg taatatttaa ttataggaaa catacaatat tattaagtaa
    32281 tgaatcttat ataattaaac atgaaagagg gttttagtat gaaaaaagca cttatatctt
    32341 tattagtttt atcttgtttg ttattaactg caccagtaaa tgaatcttct tatgcagatt
    32401 ctcaaaagaa tgttcctgta agccaaaata ctaaattgac tcaagaacaa gctaaagaac
    32461 ttttagtaaa atacaacaat gaagttgatt acatatatca aggaaatgct agtgattttg
    32521 aagcattaaa agctaaaaat cttaatggtt atgtattttt accagatgca gatggtgata
    32581 ttggttactt tgtaaatgaa aatacttctg agatatatta cttccaccca agtggatatc
    32641 ttgaattagt taatcaacaa caaaataata aataactaat aaaaaatgga tgcaaattga
    32701 tataaatttg acatccattt tctttttatt ttaaagttgt ttctttttat aaactttaaa
    32761 tactttttta atcctgtgct tgctctttta taagtttctt tatatctaca gcaggttcat
    32821 cttccatttt tataactttt ccttcccatg ttctaagcat aacgtaatct gttccttttg
    32881 atacaaggta ttgcggaagc aaaggagtct ttcctcctcc ttttggcgca tttactatat
    32941 atgttggtat agccattcct gatgtatatc ctcttaaata ctccatgatt tcaagaccat
    33001 catctactga tgtattgaaa tgttttgttc cctttacatg tttactttgg aatatataat
    33061 aaggttttac tcttattttt agtaattctt gatttaaaca tctcattaca aatttatcat
    33121 tatttattcc atttaataat actgcctgat ttcctaatgg aactcctgca tttgctaact
    33181 tttcacaagc ttctttagat tccttagtta tttccattgg atgattaaaa tgagtattta
    33241 tatatacagg gtgatatttt tttagcatat tgcaaaattc atctgtaatt ctttgtggca
    33301 ttgtaacaag agtcctagta cctaatctaa cataatcaac atgtggtatc tctttaagtt
    33361 ggctaagaat ccactctaaa taatcatctt ttaatgtaag agcatctcca ccagttacta
    33421 gtacatctct tatttcttca ttttctctga tatagtctat agattcttgg ataatagctt
    33481 ttgacttatg agaatcttgt tgtccaatat ttcttcttct ctgacagtgt ctacaataca
    33541 tagcacactc atttgttaca tttattatta atctatctgg gtatcttcta gttatacatc
    33601 ctgctgggtt tgtatactct tctcccattg ggtccaaatc ttcttgctca tcttccaact
    33661 ctatatgtgt tggtatagac agtaatttta ttgggtcaca tatatcttct gggtctataa
    33721 gactcaaata atatggagat attgcccatc taaattgagt accaacctct tttatatatt
    33781 ctttttcttt tttagttaga gttataattt ttgataaagt atctacatct gttattctat
    33841 ttgataattg ccatttgtag ttattccaat cctcttcagt tccatttaag atacttaata
    33901 ttttctgttt tcgtttattt atctcttctt ctttttcaag acctctgtta atcgtttttc
    33961 tagcctgaat ataatcatca atttttgatt ttaattcagc agctctctct aaggatattc
    34021 tagtttgttc attcataatt ttttgctatc aaagttaact acttgtatta tttgatagcg
    34081 tcctcctttc tgatgtgttt atatcattct gataggcaga tttaattttg ccatatagat
    34141 tttataacaa atgaaattga attaattttt gcattttttc tgttatttcg cacatttatg
    34201 aatatttttc aaccttcgtc aataaattac aataaattat gcattttttt taacaaatga
    34261 ttaatttttt ctatttttaa gaattatttt accataaaac gattatattt tcaagattat
    34321 taaaattaaa ttttttaata aaaagtaaag tatttcatta aataataaaa gcggaggttt
    34381 attataataa atttctccgc ctttttattt atttaattaa ataatagtaa aataaattta
    34441 actcttaaga agtgacttta ttatattaag ccctttttct aattcttcta catcttttgg
    34501 agctgttaca caaatacgaa ctgcttttgg aactgtagag tttcctactg aaaatcgttc
    34561 agcacaatat acttgaactc ctagattttt tgcagaaatt tcaaatgatt ttccatccaa
    34621 ttcatcagga agaagtaacc aacgaaaact gcaattctta tcaccaatca aattataatc
    34681 tgacaaaact ttatcagcaa cttcatttct ttctataatt gcatctcttt tctcttctat
    34741 aattttatct ataataggag aatataataa acgatgtact atttctgctg taaatggaga
    34801 aaccattaga ttcatattat atagtgcatt ttctaaattt tctatatatc gttttggagc
    34861 aactacaaat gctactctaa gaccagcaca taaaaattta gatgtactaa aaatatatat
    34921 agtattatca ctagctaaag aaaatatggg agtttgtata ccgtctctaa aaatgctatt
    34981 tatagcatct tcaataatta ttatattata ttgctttgct atcttggcta tctttttacg
    35041 agataaatca gacatagtat gtgtcgttgg attatgataa tctggtataa gatatatgcc
    35101 ttttatattt tcatttttac aataactttc aagatattca atcgatattt cattattttc
    35161 ttgaggaatt gggactaatt gaatacctat cattttagct attgacttaa ttccagaaaa
    35221 acttaaagaa tctgtagcaa ttctatcacc agctttaaat aatcctagta aagttgcaca
    35281 tatagcattt tgccctcctg tagataataa tatattttct tcactggtgt atacattatt
    35341 tctttctatc cattttgcaa ttgacttttt ttgcatatat gtgccagatg gagacatgta
    35401 ttgtaaaaac ctatccattt ctggttgttt tagtacattt ttaataaagt caataatata
    35461 tgtattttgc tcatatggag gatgaactgt ccctaattca attatgtctt ttgattccgt
    35521 ttgatataat aatgtattac ttacatttac atcagaagaa atgaatgttc cctttcccac
    35581 ttttgcacat attagtccct tttcttcgca aagtttaaat gctcgtgtta ttgtacttaa
    35641 gtttaaatct agaaaatctg ctaattcccg ttgtggaggc agtttatctc caggatttaa
    35701 atttcctctt ttaatatctt cttctaataa cattgctaaa gttttatata ttggtggttt
    35761 tcggttatct aattttggtt tccatgtcat aggatagttt tcaaaagaat ttattggcat
    35821 ataattactc ctcttaatta taattgtact ccatacaatt ttattattgt atgcttctat
    35881 tttagatgct atacttgtat ttgtaaatag ctatttcaaa ataaataaat aagagaggta
    35941 taatatgaag actataggct taattggtgg tatgagttgg gaaagtacca ttacatatta
    36001 tcaagttatt aatacagtaa taaaagaaag attaggtgga ctacactctt ctaaatgtat
    36061 actttacagt gtagattttc aagaaattga agaatgccaa tctagtggaa attgggaaaa
    36121 aagcgccaaa attttagctg atgctgcaat taaattacaa gaggcagggg cagattttat
    36181 tgttatctgt actaatacta tgcataaggt atcagataaa atacaagaaa gtatacatat
    36241 tccattactt cacatagcag atgtaactgc tacagtatta agggaaaaag aaataaaaaa
    36301 agtggcctta ttaggtacta aatatacaat ggaacaagat ttttataaaa atgtaattat
    36361 taataatgga attgaagtct taattccaaa tgaagaagac cgtataatag taaatgacac
    36421 aatatttaac gaactttgct taggaattat atctgaatct tcaaaaaaag ctttcttatc
    36481 tattatagat aagcttggca aacaaggtgc agagggtgtt atattaggat gtacagaaat
    36541 aggtctttta attaaacaaa atgacacttc tattccacta tttgatacga cagtaataca
    36601 tgcaatagaa gccgcactaa gttcaattta agacaatatt gattaataaa aaaaatatct
    36661 atagatttgt gtaaataaga ctttttataa tttatattac tagagatttt aaacctatat
    36721 attgtaacaa aaaaaggata gtataattac cctatactat cctttttctt aatttaatat
    36781 ttaaataaac tcaacacttt caatttgtat acaaatctat cttacattta taacatcact
    36841 attttgagag ctatttccat tataatttgc aacaacataa atatctagat ttaaatctcc
    36901 ttcaggaata cttatagtat gctcatttcc ctgtatatca ctttgatata gattaaattc
    36961 tccatctcca ttagtattat tttttacata tatactgtaa gtacagtcct tatgagatat
    37021 actactccag ttaactctaa tgacattatt atctctatca acattagctg atatattttc
    37081 taccctcata tctttaatat tattatcatt atttgaattt ccatttccat tagtaatatt
    37141 tattacatta cttggcttag ttgtatctga atcctttttc cctatgacat aaatatcaaa
    37201 atctatattt tgattaccct tcatatctac ttctatagtt gcatatacct tgttagactt
    37261 acctaaatca ctttcattta tagttataga tttaattttc ttaaatgatt ctcctgacaa
    37321 cttatcttta agatatatat cataatcaaa gacatctttc atataatccc attcaagttt
    37381 aataataact ttattgttta ctattttatg tgaaccaacc aagttttgta tatagtcatt
    37441 cgttatagtt tctttgacta ctgttactgt tattacttta gatttatctc cacaagttat
    37501 tctcacccta tctttacctt ctctaagacc tacaacattt ccattactac ttaaacttat
    37561 tgctctttct acaggtaatt ttgaatctac taactcataa tatatttttg gtggttctaa
    37621 accaggtgga gaagttatta tatctggttt caatttttca ttttggtcaa cttctatttt
    37681 tatatcatcc atatttattt cagcaactct agcttgaatc ctaaatgttc tctcaatttc
    37741 tttatttcca cacttagcat atattgtagc tttaccaact ccaactgcac ttactttatt
    37801 tcctttgatt tttaaaatat tttcattgct actcttataa actggcttat atgatttttt
    37861 aaacttatca taatcaactt ctattgtact ttctatagta gcactatcac caacatgttt
    37921 taaatcccca ttaatctcca tatctagtga ttctacctct gggtctacta cttttatagt
    37981 agcctttttt aatactcttg aatttttcat gatacccaca gaagaatacc caacctttaa
    38041 tgctgtcatt ttatttccat ctatttttaa agtccctgag ttatccaata cattaaacca
    38101 aatatttgga tttttagcat acgaaggaac cttaatatat tcacctgtag acaaagtaac
    38161 tgtttcattt aattttacta catatttatc tatatcttta acatattccc catctacaat
    38221 ttcaatttct actcccttta ccattggtct aagtattcca aatttaaaaa ggtagtactg
    38281 cgcaacaagc aatataggaa tagatataat tatggcaatt tttctgtatt tcttattttt
    38341 tacaaatatc atctatccac ttccttttag cctaatatat aatcatttgt tttgtaagca
    38401 atctacctaa gttaaatatt gggataaact ggaaaattat tatatttcag aatatcccta
    38461 tactaattat aaaccataaa tttaaaattt aaaactaaaa ttaatgttaa tttcttacta
    38521 ttacaatagt ctaattttag tgaaataaca tttgtcttgt cagctgaaaa gttatataat
    38581 taattaaaaa gaaattatac ttatattata tgaggaggat tttaaattgg aaatcaatta
    38641 taaaaaaaga aatttcaaat catttgctaa aagaaaaaat attgaaattt ctgttcaaag
    38701 gtatcttatt gacgcactaa gttatatggc acttggtctt ttttcgtccc ttttaattgg
    38761 aacaattttc aatacattag gagataattt caatatttct ctttttactg aagttataag
    38821 ccctcttgct aaacaggtta ctggtccagc tatagcagtg gctattgcgt atggacttca
    38881 agcacctcca cttgtactat tttcttgtgc acttgttgga gcttgtggta atgaattagg
    38941 aggacctgtt ggagcatttg tcgctacact ttttgctgta gaggttggaa aattagtttc
    39001 aaaagaaaca aaaatagata tattggttac acctgctgtg acaatactaa ttggagtact
    39061 tgtagctgaa cttattggtc cagctgtttc tgcttttatg acttcatttg gaaatataat
    39121 aatgcaagct acaactatgc aacctataat aatgggagct ttagtctctg cacttgttgg
    39181 aatagctctt acattaccta taagtagtgc agctatttgt atgatgttaa gtttaggtgg
    39241 tcttgctggt ggtgcagcaa ctgtaggttg ttgttgccaa atggtaggct ttgctgttat
    39301 gagctttaga gaaaatagag ttggtggcct tgtggcacaa ggtcttggga catctatgtt
    39361 gcaagtacct aatataataa aaaattggaa aatttggata ccacctactg tagcttcaat
    39421 attacttgga ccattatcta caacaatatt caaaatggag aatattccta ttggttctgg
    39481 tatgggtact tgtggattgg ttggacaatt tggaacagta actgctatgc aaagtgctgg
    39541 aaaaggaggt atatctatgt gggttggtat acttttactt aattttatac ttcctgctat
    39601 tgtaactttg ttgatttcag aatttatgag aaaaaaaggt ctaataaaac ctgaagattt
    39661 aaaactagat gtataaatta aaaactactc cttttactta tataaaagta aggagtagtt
    39721 ttttttatga aaattccaac atttttttta atagccaatc catagaacct ttaacttcat
    39781 tccaaaccac tatgttttca tcatagtaat agacagcttc tgccttttct tcctctgtaa
    39841 caaattctct accaaaagca tttcgccaat ctaacaaata taatccaacg tacttatttt
    39901 tacttctagc aaccttaata gaatcatttg ccaaaaacat acttttattt ttaaagtaat
    39961 aaagagcttt actgtacatc catgtagcag atttttcttc tttataaagt tcaaataatt
    40021 tatttattga agttctatca tttaatctaa ttaatacatt taataatttg tatcttattc
    40081 cctgctcatc actaggatta atttctatta aatattctaa tacttttttg gactccttta
    40141 gattattaat actttctaac tttctagcaa gggattcaag tgctatcata taggctctgg
    40201 tagataaacc tttccaaaat ccaagttctt tatcttcttt agtaatcata cttttaccta
    40261 tatttacagc ttcctccata tatctaattg attcaatatc actattcgca tcttgtgata
    40321 gcttaatata tcttttatat agtatttttt tcttttctaa atctaaatct acagactttg
    40381 atttggaatt ggtctctttt acccaaaatt ctacattaga aatagctata gtatcttgat
    40441 ttaaaaagtc aatactacaa acatctttta ttaaagacga tttattattt atagtattta
    40501 aagaaacacc tatttgtttt gaaaactctt tttttgttat atgtagtgga tgcttcttat
    40561 tgtataaaag attttgttct aaaactatat ttagtatagc tgaagaccat atatcagctc
    40621 tagaagaatg aattatctct ttattattat ctaaaaggtt ttgcaaaatt ttaaagctta
    40681 tatctttgta ttcttgattt aaattggagt tgcaaaatct atttatcttg tccttaattt
    40741 cttctatctt gcctgtcatg actcctcctc catattttac atatataaaa ctagtgctac
    40801 aaactcaaga tcttcttctc ctatattttc tatagaatgt ttttcgccat cagctgtata
    40861 cactacatca ccctcattta cttctacaaa ttccccatta tcaaatactt taccttttcc
    40921 ctttagtata taatatactt caaaatctgt tgtatggtca tgcacaccaa ttgaacttcc
    40981 tactggtaaa gctactcttt taaatagtct tccttttcct ttaagctctt cattttgtat
    41041 tatgttttca agcttaacct ttcctatacc accatttaaa ttttctttta cttctacttg
    41101 taattcagat ttctttctta tcatagctca tcccacctgc caaacaatca atctaactac
    41161 taaattggca tatcctttcc ataaatatgt ttgtatttta atataataat aacatatatt
    41221 atcataaaaa aatattactg aatacacaaa aatgatacta tttttacata tatttcgact
    41281 taattaaatt atcttacaag ttgacgtcta tatgaaaaaa acatatactc atatagtatg
    41341 gtaaaatttt gttaaactat ttatctggag gttctcgaca tatgtcagtt ttaaaaacgc
    41401 aagaaaaact taaacaacct agagatttat atttctgctg ttcaatattt acctttgtag
    41461 gacttgcata ctatggtatg aaattggtat tattattgtt tttagctgaa caagtctcca
    41521 aaggtggttt agggttaaca ccagcagaat ccgcatcaat gcttgcatct tttttagcat
    41581 ggacttattt ctctccagtt attggtggat gggtcagtga tagattttta ggtcccaaaa
    41641 actgtattat acttggaatg ctaatggttt caattggtta ttttctagga tacatagcaa
    41701 atagtaagat ggatataaac atcatgatat ttttatctgg attaggaata ggtttttata
    41761 aaggaaactt acatactatg gttggaaact tatataccaa tgatgaccca agaaaagatg
    41821 gagctttctc tattacgtat atggctacta atctaggtac tttatttggc cctttaatat
    41881 gcggtttagt tgctaatgaa tggtttgcta tgaaaagcgg attatctata agtatgtatg
    41941 gatatagata tgtattttta ttttcatcaa ttacggttct aataggttta ttcttcttta
    42001 tatttggaaa cagaaagttt tataaacctt tgaataatga ccctagtaac gggagagagt
    42061 ctataagaaa atcactagaa tataagaaaa tgctcgcttc aagaccttta actttgattg
    42121 agaaaaaaag aataatagta atattggtgt tagctttttt cacagtattt ttttggatag
    42181 catataacca agcctctatg tctattgcac tctatacaaa ggaacacata aatcttactg
    42241 ttggcaattt tcaaattcct acttcatgga tagattcata caatggtctc ctttgtgtta
    42301 ttcttggacc tatctctgct ttagtatggg taaaattatc tcaaactaaa aaaggcgatt
    42361 taagtgtgcc aaaaaaaatg agcttaggtt ttatactatt agctatagca tttttgttta
    42421 tgatagtagc agttatacaa acaggaacag accctaattc aattcacaaa gcaagtgtat
    42481 tttgggtagt tggattttta acattccaaa gtataggaga aatttgcttt tctccagtcg
    42541 ggtatggtat ggtaaataaa ctttcgcctg aaaagtatat ttcattccta atgggtgttt
    42601 ggtttttagg caaatttgca gcaaataaat tatctggcta tacgcaagct attatagacc
    42661 aacttggtat gttacaagtc ttcatagtga taccatcatt tttattgata tttggaatta
    42721 tactacttat aatgaataaa aaattagtag aattatcaaa ttaaaaattt tttaaagtta
    42781 ataaaatata caataaaaaa tcgaggcatt ataatacctc gatttttatt gtatctagta
    42841 caaactatat agctctagtg taaactttta accattctct ttcttcatca gttaagaaag
    42901 gtgatatttt atcataaact aacttatgat accagtttaa ataatctttt tcatccttat
    42961 tcattaattc tggtactata ccatctaagt caataggagc taacgtaaca acttcaaatt
    43021 ccataaattg accatagaaa ttcttttcag cttttctaac tacaatttca ttctctgttc
    43081 ttataccatg agaaccttct atatatatac ctggttcatt agtagttacc attccttctt
    43141 caagaactgc actatcaaat ctttcaggta ctactctcca tctaaatcca tttggagctt
    43201 cgtgaacatt tagcacaaat cctataccat gtccagttcc acattgataa tcaattccca
    43261 tattccacat gcaacttcta gatagtatgt ctaagttata tcctctacaa ccatgcaaga
    43321 acttagcctt agaaagattt atcattcctc ttgctacaga agtaaagtgt agttttaatt
    43381 catcacttat aggtcctaaa actgtagtcc tagttatatc agtagttcca tcataatatt
    43441 gtccacctga atctactaaa aataatcctt ctgcctctaa tttataattt gactctggag
    43501 ttgcactata gtgcatcatt gcagcatgct ctttatatgc agcaattgta ttgaaacttg
    43561 gctcaaagaa accttcttgc tctcttctta agtcctctaa tttttgagta gcacttattt
    43621 cagtgatttc catcttacct acatttttct ttaaccagta cataaattta gtgaaagcca
    43681 caccatcttt tacatgtgaa tttcttatat tttctaattc tacctcattt ttttgagctt
    43741 taaaaaacat tactggattg aactcatcaa ctttctcaac ttcacaaggt atattattgt
    43801 aaattgtata actaagctta gttccatcta ataaaacttt ttcagttttg tctatatttt
    43861 ttacaaattc atacacatca ttatatggct ttatttgaac attttcctta gctagttcat
    43921 ctaatatttc ttcatttaac ttactttcat caacaaataa ataaacttct tttaaagtta
    43981 ttactgcata agaaagtaca actggattat atttaacatc tccacctctt atattgaata
    44041 accatgctat atcatcaaga gtagttatta catgagtaga agtacctttt tcactcattt
    44101 tttctcttaa tctagctaat tttgagctaa aactttctcc acaatattta acatctaata
    44161 aaaatgcttt actatctgat aaagcaggtc tatctggcca tataccatct attaaatcat
    44221 actgatattc tatctttata ccttttttag ataatttttc agctaatgta gctccttctc
    44281 tagcagatat aactctacca tcaaatccta aagttcctcc ttcaggaata tttttatata
    44341 aatattcatc tgtagttggg caaccttctt gtcccatttt gaataatttt attgtacttc
    44401 cttctaattg agattctgcc tgtataaaat atcttccatc ggtccataac ccagcttcgt
    44461 ctttagtcac aataaccgtc cctgctgaac cattaaaccc tgatataaat tctcttgatt
    44521 tgaagtaatc tcctacatat tcactttggt gattatcaga agatggaatc atataggcat
    44581 ctatattttt ttcttccata aattttctta atccacttaa tctatctttt atattcacaa
    44641 caagacctcc tcgaaataat agtttgaata actaaatata taagtatgta aagtttatta
    44701 cataaatatt ttttgtataa tttatataaa tcttatacac tctaattatc acaattataa
    44761 ttttaattat aatatctatt tgtgtaaagc tttttttcat aatcaaagag gaaattacat
    44821 ttaattttat atttaaatgt aacttccttt atttgaagta attcatctat ttacaataat
    44881 tcgtcatgaa ttatttttct tttgttagta aatccatagc cattaatctt gttaatacat
    44941 ttcttattcc caataatatt aaaccaaata ttattaaaac tgttgctatt cctccaaata
    45001 tctgtaaaaa accaagtgtg gctgtaaaac cagcaatctt tcctgataat aaacttgcaa
    45061 aaaaacttgt tagataccag gctcccatag ataatgtagc atacttagct ggagctaatt
    45121 tattgaacat tgcaagtcct ataggagata agaataattc acctactgtt gtaaatatat
    45181 aagtcaatac tataaacaat atactagcct ttaccattcc atctggacca ttagctaatg
    45241 tagcaaatac cataactata aacccaacac ccattattat cattcctaat cccattttaa
    45301 ctggaataga gaaatcacct cttttggtat gagataattt aacccatagg gtagctaata
    45361 ctgggcataa agccacacat aatatagcat ttagtgatgt aagccatgct actggcattt
    45421 caaatccaaa tagagttcta tttacattat cccttacata aatcataaaa gaactttgtg
    45481 tttgatagta tgctgtccag aataagacca caaataaaaa taaaataacc attgctatta
    45541 tcctattttt ctccaactta gtaagtggtc catactcaat tttttcacct ttattacctt
    45601 tagtcacagg atattttccg acttcattta ataattttgg tgcaagaagt gtaaatataa
    45661 tcatacctaa catcatgcct ataccacaaa ttaaaaatgc catcttatat ccataagata
    45721 ctatatctcc acttgctcct cttactgcaa atacattatc agaaagcaat ccacatatta
    45781 ttggagctat aaatgctcct acatttactg ccatatagaa taaactgtat gctgcatctt
    45841 ttcttgacac atcagtttta ggatatatat ccccaactat atttgaacaa cttgatttaa
    45901 aaaaaccatt tcctaaaata attattgata gacctaccca aaccatggca atagatgtag
    45961 atgaagcaaa tagtacgaaa tctcctattg ctattaaaag tgcacctata gaaatacatt
    46021 tttgttgacc taaaacttta tctgctaacc agccaccgac taatggtgta agatatataa
    46081 gacctgtaaa taagccaaac atggatgttg cttcttgagt agaaagacct aaaccacctt
    46141 gagctacact tgctgtaaaa aataatacca ataaagctgt taaaccataa taactaaatc
    46201 tttcccacaa aattgtcatg caaataagcc aaaaactctt cggatgacca ctttttttag
    46261 atacgctttc tgtacttgac ataaaaaaac gccccctttt gttttatagt tgaatttaat
    46321 attaatattg ctttaaccat tttagcacat acttataata tcatcttttc tatgtttact
    46381 caatagatta tcgcctgtat tttcattttt ttattattat ttttaaaat
//
LOCUS       DAELSY010000011        46378 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.011, whole genome shotgun sequence.
ACCESSION   DAELSY010000011 DAELSY010000000
VERSION     DAELSY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46378
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.011"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            104..985
                     /locus_tag="KPG09_000536"
     CDS             104..985
                     /locus_tag="KPG09_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBG0965165.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKKGVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(1298..2077)
                     /gene="hisJ"
                     /locus_tag="KPG09_000537"
     CDS             complement(1298..2077)
                     /gene="hisJ"
                     /locus_tag="KPG09_000537"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBG0965166.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEDLIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(2617..2967)
                     /locus_tag="KPG09_000538"
     CDS             complement(2617..2967)
                     /locus_tag="KPG09_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBG0965167.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(2983..3399)
                     /locus_tag="KPG09_000539"
     CDS             complement(2983..3399)
                     /locus_tag="KPG09_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBG0965168.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(3418..3609)
                     /locus_tag="KPG09_000540"
     CDS             complement(3418..3609)
                     /locus_tag="KPG09_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBG0965169.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(3760..4575)
                     /locus_tag="KPG09_000541"
     CDS             complement(3760..4575)
                     /locus_tag="KPG09_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBG0965170.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(4622..5560)
                     /locus_tag="KPG09_000542"
     CDS             complement(4622..5560)
                     /locus_tag="KPG09_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0965171.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDASNKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYIYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(5557..6429)
                     /locus_tag="KPG09_000543"
     CDS             complement(5557..6429)
                     /locus_tag="KPG09_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0965172.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEK
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(6454..8382)
                     /locus_tag="KPG09_000544"
     CDS             complement(6454..8382)
                     /locus_tag="KPG09_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBG0965173.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNSL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(8494..8871)
                     /gene="gcvH"
                     /locus_tag="KPG09_000545"
     CDS             complement(8494..8871)
                     /gene="gcvH"
                     /locus_tag="KPG09_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBG0965174.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(8958..11084)
                     /gene="cdhC"
                     /locus_tag="KPG09_000546"
     CDS             complement(8958..11084)
                     /gene="cdhC"
                     /locus_tag="KPG09_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBG0965175.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(11141..11947)
                     /locus_tag="KPG09_000547"
     CDS             complement(11141..11947)
                     /locus_tag="KPG09_000547"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG0965176.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(11997..13364)
                     /locus_tag="KPG09_000548"
     CDS             complement(11997..13364)
                     /locus_tag="KPG09_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBG0965177.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(13395..14339)
                     /locus_tag="KPG09_000549"
     CDS             complement(13395..14339)
                     /locus_tag="KPG09_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBG0965178.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(14359..15129)
                     /locus_tag="KPG09_000550"
     CDS             complement(14359..15129)
                     /locus_tag="KPG09_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0965179.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(15185..16570)
                     /gene="lpdA"
                     /locus_tag="KPG09_000551"
     CDS             complement(15185..16570)
                     /gene="lpdA"
                     /locus_tag="KPG09_000551"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG0965180.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVIEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(16614..17495)
                     /locus_tag="KPG09_000552"
     CDS             complement(16614..17495)
                     /locus_tag="KPG09_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBG0965181.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMQKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(17531..18205)
                     /locus_tag="KPG09_000553"
     CDS             complement(17531..18205)
                     /locus_tag="KPG09_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBG0965182.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(18242..19114)
                     /locus_tag="KPG09_000554"
     CDS             complement(18242..19114)
                     /locus_tag="KPG09_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBG0965183.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(19206..19838)
                     /locus_tag="KPG09_000555"
     CDS             complement(19206..19838)
                     /locus_tag="KPG09_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBG0965184.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKIEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(19943..21619)
                     /locus_tag="KPG09_000556"
     CDS             complement(19943..21619)
                     /locus_tag="KPG09_000556"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBG0965185.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(21868..22647)
                     /locus_tag="KPG09_000557"
     CDS             complement(21868..22647)
                     /locus_tag="KPG09_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0965186.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(22957..24876)
                     /gene="cooS"
                     /locus_tag="KPG09_000558"
     CDS             complement(22957..24876)
                     /gene="cooS"
                     /locus_tag="KPG09_000558"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG0965187.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(25483..26394)
                     /locus_tag="KPG09_000559"
     CDS             complement(25483..26394)
                     /locus_tag="KPG09_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG0965188.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(26510..26632)
                     /locus_tag="KPG09_000560"
                     /pseudo
     CDS             complement(26510..26632)
                     /locus_tag="KPG09_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            complement(26715..27857)
                     /locus_tag="KPG09_000561"
     CDS             complement(26715..27857)
                     /locus_tag="KPG09_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0965189.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYKVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(27875..29629)
                     /locus_tag="KPG09_000562"
     CDS             complement(27875..29629)
                     /locus_tag="KPG09_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBG0965190.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMKINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKQSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            29765..29971
                     /locus_tag="KPG09_000563"
     CDS             29765..29971
                     /locus_tag="KPG09_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965191.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(29976..30899)
                     /locus_tag="KPG09_000564"
     CDS             complement(29976..30899)
                     /locus_tag="KPG09_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965192.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(31153..32853)
                     /locus_tag="KPG09_000565"
     CDS             complement(31153..32853)
                     /locus_tag="KPG09_000565"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBG0965193.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(33264..33677)
                     /locus_tag="KPG09_000566"
     CDS             complement(33264..33677)
                     /locus_tag="KPG09_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG0965194.1"
                     /translation="MILVSACLIGINCKYSGDNNENEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKIKSEKDL"
     gene            complement(33745..36123)
                     /locus_tag="KPG09_000567"
     CDS             complement(33745..36123)
                     /locus_tag="KPG09_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG0965195.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSEISAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRNLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            36440..37891
                     /locus_tag="KPG09_000568"
     CDS             36440..37891
                     /locus_tag="KPG09_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBG0965196.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(38095..40608)
                     /locus_tag="KPG09_000569"
     CDS             complement(38095..40608)
                     /locus_tag="KPG09_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG0965197.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTSDEQLEVLKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTDLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKDTEKLKVGVLLNKPPFQNADKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLEDMAKKHEIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLNGK
                     RIALAKGIDYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYEAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKSDKESENGNIENERDKIRREYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRITSKIIYNDRKIPMYVIGKIANIDSEKEEKNRLLDKVQR
                     DSLTNIYNAAYIRKLTTQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLVGDKVLIDV
                     AKVLDESFAKDDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVITKRGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(40831..41652)
                     /locus_tag="KPG09_000570"
     CDS             complement(40831..41652)
                     /locus_tag="KPG09_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0965198.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMIVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEMR"
     gene            complement(42171..42647)
                     /locus_tag="KPG09_000571"
     CDS             complement(42171..42647)
                     /locus_tag="KPG09_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBG0965199.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVGNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(42681..43259)
                     /locus_tag="KPG09_000572"
     CDS             complement(42681..43259)
                     /locus_tag="KPG09_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG0965200.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(43363..45981)
                     /locus_tag="KPG09_000573"
     CDS             complement(43363..45981)
                     /locus_tag="KPG09_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG0965201.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIETGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MNRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMASSISIDSSISIDSSISIDSSISMDLDTSSSLGIPKELMGSNTNNIDTLSNTSK
                     IDTLNKNHYNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFK
                     KKDLNIDCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGY
                     APMMVTEYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSK
                     VTCMLDNLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKK
                     LDEIGTVAGHYYKGVE"
BASE COUNT        14135 a         8939 c         5125 g        18179 t
ORIGIN      
        1 tttttacaac agttttgtaa ttaaaaacat attgaaggta aatttaaagg tactatatac
       61 tataaatatg tcgacacaat aaatttaaaa aggagaacta aatatgaaaa aaagattaat
      121 aataatgata ttgtctgttg tcttagtact atcaagtata cttacgatat ttgcatattc
      181 aaatattaaa tataataata ataaattgat atattctaat atgatagata aaaaaactca
      241 aaatagtgta aaggaaatcc taaaagaaaa taaaattaat gaaaaagaca tagatacatt
      301 tattaaagca gtcaacaatt ataataaatt gcaagttaaa atacttcaaa ataatataaa
      361 tatatctaaa tctggatatt cttcaatcaa tgcaaaacaa gttccatata acctagaaaa
      421 attgcaagat aattgggtga aaaaattccc tgattatatg gatgtaaatt gtagaataac
      481 tgcttttaga ctttttaaag attttataaa ttcaaataag aaatttactg gagactctat
      541 tgatttgaat gtcgacttag acactataat gaataataaa gatgctaaat ttagcaccaa
      601 agatgtagaa aaatttatca acttcttttc tgcaatacca gctaaagata cagatgatac
      661 aatcaaaatt gcagaacaaa taaaaaatga atggaaaaaa agaaaaatat cttttaaaaa
      721 taataaaaat atatctatta taaatggatt tcttcgttat ccagaaacta aaaacgtatt
      781 tataggtcat acaggcatat gtattaaaac taaaaatggc attctatttt tagaaaaata
      841 tggtgttaca tcaccttatc aagttactaa atttaaaaat aaaaaaggtg taaaaaatta
      901 tatgtttaat agattgaaga tgtcagaagg tgaaattgaa ttgcctgacc ctataataat
      961 ggaaaacgat aaacttatga aataataact ttaaacgcaa aatagtattt ataattacta
     1021 agtcaatata atagagaagg tctatagatt tgtgtaaata caatcttatc tacaatctta
     1081 tataagaatg tttatataaa taagatagat taattctaag tcctattatt ctattgaatt
     1141 ttattattat tttacttaga ttctattgtt attttactta gaacttattg ttattttatt
     1201 taagttctat tactattttc aatcatataa atttacaaaa gactgtacat aaatttattt
     1261 tatacaaact tatctacagt cttttgtgtg aatatatcta aataatatcg tatttattta
     1321 tttcctctgc acagtgagaa tcagaaccta aaactaatgg aatatcgtat ttttttacca
     1381 actctaataa gtatccatct atataaggct ctttgcaata ctcttttcta tttccagaaa
     1441 cattataatc aagttcataa cctttttctt taattaactt tattaagtct tcaagaattg
     1501 tatttccttt ataatcgtat ggaaatacct gattaaactt tctaactaaa tttaaatgac
     1561 ctattctttt tggtttatgt atacctaaat ctgaatttac agcttttatc aatgtttcat
     1621 aatacttatt ataaacagct tcaatacttc ctaatttgtc gattaaattt tgaaactcct
     1681 ctacactgta atctacacaa taataatcat catcaatttt aattatatgt actgataaaa
     1741 gagagtcttc taattccttt ccatatttat ttaaattctc ctttatccct tcttcgtatc
     1801 cttctataaa atccacttcc gacccaacat ttatcttgat tttaccctca tacttttctt
     1861 tcaacttctt gacatcatta aaatactttg gtaaatcttc aatactcata caactatcat
     1921 tttcaggagc agggtctcta aatccatttg catacggaaa atgttctgta aatgtcattt
     1981 ctgttattcc aacatccatt gctctttgta catatttttc aaaatcatcc ttacttccat
     2041 gaggacaata aggtgaatgt atatgaccat ctttcataaa taattcactc cttttttaaa
     2101 gaaaaaatct aatataaatg cataaatttt ttaacttcaa aataatattt ttaatcatgc
     2161 taatttttat tatatctaat attagagtaa ttttaaactg ccttttaagt atcttatctt
     2221 tagtactatt ttcaacttaa cattaatatt attttttaac ttaacattaa tactatcttt
     2281 aaatagtaat actcaatata taattaatca aataactcaa attgtttgat gaagcttttg
     2341 cagtatttaa agaatatata attatttaac attaaataat ccaatcttgt aataaaattt
     2401 actcattaaa gtatattaca gaaaatatct caaaataaac tttttagttc atgatatact
     2461 cttttttgca tgaaattaga tatattttta tcatttcaac aatgaaaggg aggcttacat
     2521 ccatcttgag ataacccctt agataaaatt tttaataatt aaacatcata aaatataaaa
     2581 atcgcttata tcaaacgtat aagcgattaa ttatatttac atatgttttt tagcctctaa
     2641 caacatttta ttagcttctt ccatatactc tatagccttt tcaatactct ttgctgtttc
     2701 ttcttttcca atagctctag ccttttcaac ccactctcta aatccttctt catgagtttc
     2761 attgtgattt atccaatgta ctaaaagtat ttttaaagtt ttttcatcct tagactctga
     2821 tgattcctca tgactatgtt catggtcgtg gtcgtgagca tggtcatgtg gatgttcatg
     2881 acatatatct ccatgagtat gttcgtggca gtggtcatgg tcatgatgtc catgatgata
     2941 tcttacaatc tctttatcaa aaatcattag ttattccccc tgttatttta tattaaactc
     3001 tttttttata aggtttacag aatcttttac aagtagtgct aatggtttac cttctaatcc
     3061 aactaactcc acattttctg gcataatagg taaaagtatc ttaagtgcct cactgtctgc
     3121 aatagcttca cttatattac aagtaacttc tcccatcata gaattaggta ttattacaga
     3181 aataggtgca actattatat ttgctttttt agctgatact actatagcat tttcccctgt
     3241 agctccttta ttagcatgag ctttcatcat agaactagtt gctatagaat tagtccctag
     3301 tgcataaatt tctacatatg taggtaactc ttctctcaaa gaagaaacta tctgggctcc
     3361 aatacctcca cccattccat ctatcactgc tattatcata tgaaattccc ctattaatta
     3421 attttcttgt attattatct tatggtccat caatcttatt tctttaatta tcccatctat
     3481 tatcttttgc tctcctaata aatccgttaa ataaatcttt ccatcacaag ggtctatatt
     3541 aaccacattt tccataacct tttctaactc attattactt ttacaaatga aagctgatga
     3601 ttcacacata taaatctcct cctaatatga tttttattaa aaatcaaaaa aagattccct
     3661 taggtatttc aatactccaa agaaaccttc atgatttcag tttttgtata ttattatacc
     3721 ttaagtatat cactttaatt tatgtaaagt caattaaagt taacgctttt tattatactc
     3781 ttcattcttg tatttttcat atagttcttt aactaattgc ttctcataat cagtcaaatc
     3841 gccctccaca aactcaacat taaattgttc agctaatccc tttactatgt ttttctgtag
     3901 aatatctatg tctatttttc tgccaatttc attttctatt ccacttgctt ttttagctgt
     3961 aaatttcata gctctttctt tcaattctgg tgtcttagtt tttattaatt taaacaattt
     4021 ttctacatca aaatccagta ttattgaacc atgttgtaat aaaacaccat cttttctgct
     4081 ttgagcactt ccaattacct tcttattatt aatagtaact tcatatgatg catgtgcatt
     4141 aaaacatgca gctgataaat tttccctact tattctctcg cctctattta aattatctgt
     4201 ttctatacca cttagattca atcctttaac tagaccttca cttatatatc tataagatag
     4261 gttgatgctc ttatcgatta aaggattatc ttctcctata attatactat aagttagctc
     4321 attatcatgt aatacagctc ttccacctgt aattcttcta gtatagtcta tattcataca
     4381 tctacactta tctaaatcta tctcatcttc caatttctga aaatatccta tactcaaaca
     4441 agctggttcc catgtataaa atctaagagt tggcttatta tgtcctttct tatatgcagt
     4501 aaatatagct tcgtcaattg ccatattcat tgctccttca tatgatttat tatgtataac
     4561 tctccactga ttcatgatta ttacgccccc atttttagta taaatatatc ctagaaatcc
     4621 tctatagatt tatttctctt attgctaaat ctatctcact ttgatttaaa ggtcttgaat
     4681 agttgtatat agtacttccg ggtctttcat tatagtatgg tctattacaa tccttacaac
     4741 ctcttatttc aaaaggatag cctttgttta tgtcttttaa aatatcattg tcaagcttta
     4801 tactatttaa gtatccattt ttaaacttaa aatactcttt tggataacct ttatttatca
     4861 tataagacat taactgtacc cttctataac tttctatgct tggttgattt atcttttcca
     4921 ttttagttcc tctaactgga gtaaatgcaa ataaactaat cgttacatca ttttccttta
     4981 aataaatata taaattgtat atatcttcat gactttcacc cataccaact attatatgtg
     5041 tacttatttt attgggatat gattttgaca tttcagttat aaattttaac ttttcatcat
     5101 agttatttcc ttttattttt tcatatagtt ctttatttga tgcatctatt gctattccta
     5161 ttttatcaac tcctactgaa aaaaacttct ttatctgttc atcattttct aattttgcag
     5221 atagtgaaat tggcatgtct atcttaccac ttatatagtt tataaagtct aaaacagagt
     5281 tatgtgcttc ttcacttgcc attgattgaa tacatattct ctttatattt tttcctttat
     5341 aagcttttaa agcatctaat atttcttctt ttgaaaactc tggccaaatt actcttgata
     5401 atttatcttt tctagtacta ctttctattg attgagaaca aaaactgcat ttatttatac
     5461 atttttctcc aatcattata tatgctgtgg taggaggtat atcgcttttt ttgtttaata
     5521 tcccaagctc tattgctgtc ccaactgata gtctaatcat aaaacgcaac actccttaca
     5581 ctcatttatt gtcatattca tttctatagc cttattcttt gcagaccttg atggtaatac
     5641 tattctattg actccacaca ttatagataa ttggtctagc tcttttctat acactcctct
     5701 tggtctcata caacctagat taatttcagt atctggtaaa ttaattctag cttcacataa
     5761 aatatctgct accccttcta actcaggagg ttctacattt tcatactcag tcccttttgt
     5821 tggaataagg actataaatg taagcttatt aggaggattt ttctgtaggt attctataat
     5881 ttcatattca ccctttattt gtcctccttt taaaccaata catatatgtg gtgctacttc
     5941 tgtatgttct tgtattgtat tataaacttc aatataatct tctttactct ttttcatctt
     6001 ataaacttct cttattgtct catcatcaaa cactaaatca aaagaaacta tatctaaata
     6061 cttacacaac tctacaatgc tatctttatc cataagacct aaatgagcat tcaatctata
     6121 tcctttctct tttaagtttt taatggtatt aatatgagta tttataggga catctccctc
     6181 ataactacaa ccgccactca atagaaagct agttatattc cttgattgta ctttttcctc
     6241 atattcttct attggcacca tattgtttaa gtagtgaccg ttgcaatggg cacatttcaa
     6301 actacattga ttgcttgtag tactaagagc tagtgtctgg ttaggataag caaactctat
     6361 tttatttcca aaattcttta atttaatatc aaatgctatt ttcatttttt cagatagatt
     6421 cttattcata gtttattcct tcttaagtac attttactct tctagtaatt tctttgctgt
     6481 tgggaactgc tctatattcg tatgtggtaa gaaacatgca gaaataaatt catccatata
     6541 acttggatat acactaagtt caacataagt tatttgatta cctatttctt caagtttatt
     6601 ttttgcatct ttacttatga gagctaagta agaaccaaca agtgaactat tccctatata
     6661 agtaaatttt tctctatcaa tgtcaggaag taaccctata attattgagt tttctatgtc
     6721 taggttgttt cctataccac ctgctatata aaccctatct aatatactaa aatccatacc
     6781 taaactatct acaagagtgt atgcacctga atatatagct cctttagctc tgatgaaatt
     6841 atctatatca acttcattga ctactatatc attttctaaa tcaaattctt ctttaaatgc
     6901 taatacatac tctcctatct catgctcatt aaatctaact ctcttattat ctaaatccct
     6961 gtatatttta cctcttctat ctattacacc tttagtaatc atttgacaga ttaaatcaat
     7021 tataccagaa ccacatatac caactggtgc acattcatca ataatcttta atgttggctc
     7081 taaagtttct ttatcaataa ttactttctc tattgctcct gctgatgccc tcattccaca
     7141 gcttatccct ccaccttcaa aagcaggtcc tgctgaacat gcacagctca tcatataatc
     7201 tttatttcca aatacaatct ctccattagt tcctaaatct ataaataaaa cattttcttc
     7261 acttgaccat atccctgctg ataatacacc tgctgttata tctcctccta cataacttgc
     7321 aacagatgga gctaagtaca catatgcact gtcatttaca aaaagaccaa cattttcacc
     7381 cataagtttt ggagatttta aaaatggtgg tatgtaaggt tcttgtctta agaagtctgt
     7441 atacacaccc aagaataaac ttgtcatagt tgtattcccg gcagccacta atgtaactac
     7501 atcttcctta tctattccat ttctttcata tattgttttt aatagtggat ttatagtttc
     7561 ctcaactata gctttatgaa gtgtctctaa tccatttttc ttagttgaat atataattct
     7621 atttataaca tctgctccat attttatctg tgcatttcca gaagatgctt tatctactac
     7681 ctcttttgaa tataggtcta ctaaacaaac aactactgaa gttgtaccaa tatctattgc
     7741 aactccgtat aaggaatttt ctttattacc ttgttctata tttatgattg taagtttctt
     7801 ttgtttttga acatatgtta ttgtaacctt aaaatcactc tttctaaata cagttggcat
     7861 ttttctaagt atatccaatc taaaatctat ttcattatat cctaggctat ttctaacata
     7921 tctttccaat ctatctacat cacttatatt atcatctaaa tttggttctt ccatttctat
     7981 gtatttcttt tttatatttg ttttaaattg aaggttatgt tcttcaatta tttttttagc
     8041 tctttcaaaa atttccctat cctctttttt attactacct tctatcttca taccatgcat
     8101 agaagatgat actttagaag gaacttcaat ttctatatct gatattacct ttgtacaaca
     8161 agctagtata taaccttgct cccactcatc atctgttata tgtcttgttt tttctgtatc
     8221 aacttttcca ttcaataatt ttactttaca cttcccacaa gacacgtttc cattacaagg
     8281 agcatctatg aatatatctg catttcttgc aacctctagt aatatatcac cctcattaca
     8341 ataaacctct ttattgtttg gtgtaaaact cacttttatc atgttatccc tcccaatttg
     8401 ttctatatat gataaaagga aagtatagct aaatttgcgt gcactcaaat tttactatac
     8461 tttccccaat tataataata tcttaatctt taattattct aatccagcct cgtattcttt
     8521 atcacttaat aacgtatcaa gttcagccac atcagctagt tttactttta atatccagtt
     8581 ttcatatggg tcttcattta tacattctgg ctcatcttct aatttttcat taacttccaa
     8641 tacctcacca ttaacaggag atattaaatc agaagctact ttagaagact caactactcc
     8701 aaaatctact cccttagtta cagtatcttc tacttctggt gtttcaacaa acaatatttc
     8761 tcctaattga tcttgagcat agtctgttat acctatatat acaacatctc catcaattac
     8821 ttttacccac tcatgatctt tagagtattt taattctggt aataatttca tttcgataga
     8881 cccccaatat tatattattg attaaattac ttaaatcata ttaatattat atcatattat
     8941 tttaaatttt ccatcaatta cattacactt tccatagcta atgctggatg acctttttct
     9001 tctaagaaag ctaatactcc ttcagaatcg tcagctatag tttcatcaca aaccatatca
     9061 cagaagtttt ctattccagt catttcttta actgtagcat ttaatttatc agctacataa
     9121 tcttttaatt cttttggcat ccaaactatt ctttctggtc caccttctgc ataagcgaat
     9181 tttttagaag atatgaaatg tcttccgtgt cccataaatc ctggagtttg aactcctcca
     9241 cctgtcatag aagcaagttc tccgaaagtc ataccaactg gagtaacact tgcaaattct
     9301 ctgttaacta caacaaatcc gtttgcttct ggcattatac cacagataca ttcgaagcat
     9361 ccacaagaag tcattgggtc ttctagtata gaatataatg taacagattc aactgcacct
     9421 tgagatattt ggtttacagt ttcatttact gaattccata cacctgttct atcatctaag
     9481 cactcgcctt tttctattgg ttgacaagga cctgttgggt ctaattcttt agtagcctta
     9541 gcatctaacc agctaactgc tccacaaaga ccaagtcttt caggagtaac aacacaaacg
     9601 tgagctggtg caaatgattg acataagtta caagaataga aagtatcaac actttcatca
     9661 actaatgaag ctaatctttc atctctagca ttgtacttag ctattgcttc tccttttaat
     9721 tcagacactt tttcagcatc tgtaattata gttatttcac acttatcaac tactgattca
     9781 aattcatcta acatcttagc atataaaact tctcctatat gctcaagtct aaatccttta
     9841 tcaaatgcat ctttagagat tcttacccaa gccatatctc tttgtccaac gtgcattaca
     9901 ccttctatat agttcaagaa gtagtggaaa cgtctttcaa gaactggctc gaaatcttct
     9961 tgcatatttt taccagctat tttaactatt acagcaagcg gtaatcttaa tacaccatct
    10021 gcatcaactt catctatatt tggtcctatt atttctatct tatggtcttc aacttctgtt
    10081 gcttctttct taacaacaag ctcccaagct tcagttctgt ttccaccaaa ctcagcaaac
    10141 atatcgctct ttctaattct ctcaccttca aatgctgctg caaatgaaac tgggataggt
    10201 atttcagtaa cttttatttt tattcctcta gcttctaatg aagtagctac tatcttatcg
    10261 taatcttttt gagttaatag gttcattgga acttcaagaa ctgtttggtc agttataact
    10321 gggaatccta aagctattgc tccagcacct gcagatacaa ctaattcact taatggtcca
    10381 aatgcattaa caaatgcagg aactctatta gctgtgtact ctaataatcc gtttaaatca
    10441 ccaggagtta atccaccgaa tattaatgct gctcttactg caacagatac aacgtgtata
    10501 acagaagtta catcatatcc tagaggtata actcttagct caagacccat ttttactcca
    10561 gcttctatag cttggtctat aactttacca actaagaaag ttagtaaacc tttagattgg
    10621 taatctttta taacttttgc tgctgattct gcatctggac actcaccaag aacaactgca
    10681 acaccaggta tatctcctgt aactaatggt acaccaagtg atctgattat agggtcagtt
    10741 atatgacctg cacatggctc actatatgga gcatccatag ttgaatattt taatgcttca
    10801 attacttctg cagctaaagc cgtagctaaa ccagcattaa aagcatgttc tagtaaatga
    10861 gttctattta ttaaagattt tactacttct aaagcacctt ctaagtcccc taaagtgttc
    10921 atcttttgtc ctgttgcagc atatatacaa ggtaatgaat atgctgtatc agggaaagca
    10981 actttatgct cttttccatt cttttctata gcttcagcta acatagcttg agccgcacct
    11041 aaagcttgtt ctgaccctgt aaagattata ttatatagat tcattctatt tcccccttta
    11101 atatttacac ctatttttta ttaactatac cctaaaaacc ctagaaagca aatccacctt
    11161 catttaactc taagaaagag tctgcatata aagatgcccc gttgattatg tcacaagatt
    11221 ttacagtttt cattaattct gggtcacatg gattcattat agctgaacta aatccattag
    11281 ccattgccat tgctaagaat gcactatcta aaacaggtct aacatgctta ggacatccat
    11341 tagatacgtt agataatcct cctgtagtta aaagtcccat ctcagtcatc atttttatag
    11401 cttctaaaac ttctacttgt ttttcttgca taccttttat aactaagcat aatgggtcaa
    11461 atagtatatc agttggctcc attcctaaca ttaagccttt ttctaatatt tcttggcagt
    11521 aagccatacg ctcatcatta tctcttggga taccttcttt tgcacataaa cctataacca
    11581 ttgcttcgta ttcagctgct atatctatta aatcaaatct tgagccagca tcagcagagt
    11641 ttattattgg ttttgcatta gttctatcat aaactttaag tccagcttcg atagcttttt
    11701 tattagctgt atctaatgct ataggaacat tgttgaactc agattgaagt aatttgattc
    11761 cccatgtcat tatttcttcg ccatctcttt cggcaggtcc tatattaaaa tctatatagt
    11821 gtgctcctgc ttctaattgc tcttttgctc tttttaagat tggagctggg tctctttctg
    11881 ctaatgcttt tcttattgaa ggtgatatac aatgtattct ctcacctata atcataaatt
    11941 tttccattta taatgtcccc ctttaaattt aatattaata gttagttaat attttattag
    12001 tttgtttggc atgctttttc ttggtatcct tttaagaact taggtaattc catagattcc
    12061 tctggtccta taactacatt ccatcctggt aagttatctt ctatatcacc tttaagtact
    12121 gcaacttttc ctggaatgat aaggtcttta caattagtta cttcttctac cttactttct
    12181 ttaatgaaag cagagattga gtttccacca aatttaccag cagcccaaga tgtaagaact
    12241 gaatatccac ctgcatctgg tattactaac catactggaa cttttgatct ttctatatca
    12301 ccagcaacta tgaagtaagt taatgcaaag tcaacagtaa ctaatactgg agaattttca
    12361 tctgggttgt tgattggata aatctttggt tcaactctca ttggtctttg tgggtcagtg
    12421 tatatgtttt gtcttagtgc aaataatggt aatgccttag cataactaat atcatctatt
    12481 actattatag aaccgtattt tatagtgaat attgatgata aagcaacttc catcataggg
    12541 tttgaattag ataatctatt tgcaaatact attgaaggat atccaaatgt tctatcttgc
    12601 tcttttaaag ctgttcttct aacttgtata gcattagtgt aagtatcttt tatgttttct
    12661 cctgttacat ctaatactaa ttctttatat ccagcggctt gaattaattc aactgtttca
    12721 tataattctt ctaagctacc agcttttaca cctaatggta aacttgctcc tttaactact
    12781 tctatcatat ctttgtagtt ctctttagtt gctccatata ccattggtct agcatctttt
    12841 aatacttcaa cagcttcttt aactacttgt gcatcatcac aagctaaaat ataagctact
    12901 tctaatccac tgccttttat tttatctatt aattttaaat acgcatcttt atctccacaa
    12961 tactctaaaa cagccatttc aactttcatt tgttcaccaa ttcttacata gtcaactttt
    13021 ttcatgttag ctattttagc atctactgct tcatcactca tacaagtaca gaatgaaact
    13081 gcatatctat ttctacttac taatgttttt tcatgtctaa ataatactgt ttctccacct
    13141 agttcatact cagatgcacc agcaccaact tttaaagctt tcattaaagg tgcagtagcc
    13201 tctgataatt ttgctatagc gtcatcagac atatgtgggc atttacctac ttctacagct
    13261 cctgaagcaa ccttcataga gaaagccata caagttggga atccacaatc cttacagttt
    13321 ttctttggtg ttaatttaaa tatatctaaa gcttttaatg ccattatttt ttccccctaa
    13381 ccttaacaat ttaattaagc taatgcgtta accaattctt ttatagtttc tattgatttt
    13441 ggatgacgaa gtataactgc gtttgaacca cctactaaaa cacttgctgc agttgaaact
    13501 tccattgcta tagttctttc ttctggacaa ccccaatctg gctcatcttc tattggagct
    13561 atagcttctt taacatgacc tacttcaaaa gctactggtg ttatgatagg catttgtaat
    13621 gtcttatcat tttgaccaaa tgctgcaagt ctaattctat ccatagtaga tgcaacgtac
    13681 tcatatccat aaccaactgc tgaacatcca acattcataa ctatattttc acctttaacg
    13741 cctagttgag ttaacaatac gtttaattgt ttagctaggt ttatatcaac agaagattca
    13801 gctcctactt tatgtgcata agccatacca gctgatgctc ctactccttt gtagttatct
    13861 tctactgctg acataaataa gcagttatgt ccatccaatg tttgagctaa tttttcaaat
    13921 aatttaccat ctttttcatg atttcctgaa ccagctacaa ctaaaggtaa ttttattgct
    13981 tcaattactg ccttagctac atcagcacat tcatcaactg acttatctaa tccatttggg
    14041 tcagaaccat caaattttaa acaaataaaa tctgcttgtg tattagcttc aacatattta
    14101 gcccactcta ctggacaatt agctacatct ttgtataatt ccttatatga atcagtccag
    14161 ctttcaggat aaacgtctga tatttgtata cctatttttg gagaattacc tacttctcca
    14221 tcaaaactat aaaaaggtaa tacattttcc ccacctaatt taattgcttt ttcccctatt
    14281 cctacttcaa cttctgatat ttttccagaa tatttttgag tagacatttt aaatgccata
    14341 tttttttccc ccttaagcct attttaattc taatttagca aaaatttctt tcatagccac
    14401 tttagatttt gaatcttcag gtaaattaac taaaggaata cctgtagaat catattcata
    14461 tatcaattca tccataggta caacaccgat tagctctagg ttatgctttt caatttcttc
    14521 tttaacacca tcattcataa ccccatttgg aactttattt acaatcaaaa gtattcttcc
    14581 aacacttaac tttagttcct cagctaaatc tcttattcta gcaacagctt gtatgctacg
    14641 tcttgagcaa tcactaacca ataaaagtgt atcaacatgt ctagtgactt ttctacttag
    14701 atgttccata ccagcttcgt tgtccatgac taagtatttg taatgaccag atattttgtt
    14761 tacttgttct ctaagtatcc catttacaaa acagtaacat ccttcacctt ctgacctacc
    14821 cattactaat aagtcatatc cttcgccttc ttcgattata gaatttaatc taaattgaag
    14881 atattgtgcc tttgtcatac caccagggaa tgcattgcct aacttttctc tttgatttac
    14941 ttcttctctt atttctccta ttgttgcttc aacttcgata cctaacactt catttatgtt
    15001 agcattggca tcagcatcta caactaatac tggtcccttt ttgtccttaa ccaaataatc
    15061 aattaaaagc cctgtgagac ttgttttacc agttccacct tttcctgcaa cagctatatt
    15121 gtatcccatt ttaagacttc ccccttaaat ctagccacag agccttatgt actctgtctg
    15181 tctattataa tttaggtaca gaatgaacac attctccatg tacatcatga agtgcttcca
    15241 ttagcccttc agataaactt ggatgtggat gtattgcatc ccctacttgt tctactgtta
    15301 atcctagatg aacagcaagt gacaactctg tcaataaatc tgtagcatga ggtcctacta
    15361 ctgctgcacc tattatctta tcagtttctt tgtcagctat aacttttaca aatccttgga
    15421 aatgtcctat agcttgtgct tttccaagac ctctaaaatc aaattgacct acattgtatt
    15481 ctacaccttc agcttcaagt tgcttttcag tcttaccaac accagcaact tcaggttcag
    15541 tataaacaca tcttggaatt gctctataat ctactacctt agtcttacct aaagcatttt
    15601 caactgctac gataccttct ttagaagcaa catgtgctag gaaaggagta tctattatat
    15661 cgcctattgc gtatatacct tctacattag tttcaagatg ttcattaact actacttttc
    15721 ctctttccat ttcaattcct atatcttcta ctccagaatt gtcaaggtta ggtcttctac
    15781 caacacatac taatgcatat tgtgcttcta taacctttcc attagaaaga gttgcaaccg
    15841 ccttaccatc aactacttca caagtttgta ctccaatacc tgtaataacc ttaattttat
    15901 ctttcttaaa ttgtctaagt agttgtttag ctacatcttt gtcttcattt aatagtattt
    15961 ggtcaaccat ctcaactata gttacttctg ttcctaaggc tctaaagaac tgccctatct
    16021 cacatcctat aactccacca cctactatta acatagactc aggtatctct tcaagtccaa
    16081 gaacttcatc actagttatt acaacttttc catcatatgg gaacattctt ggtacaactg
    16141 gaacagaacc atttgctagt attatcttat ctgcttttat agtttctact gttccatcat
    16201 ctttagttac ttctatagtg tttttatcaa ttaattttcc aaaaccatta actaagttta
    16261 ctcctctttt ttcaaataag aactctatac cacttattaa ttggtttaca actttatttt
    16321 ttctttccat tatggcagtg aagtttggct taactgtacc atctatttct ataccaaaat
    16381 cctttgcttc ttttacagta ttcaatactc ctgaagaagc aagaagtgct ttagttggta
    16441 tacatcctgc atttaagcaa gttcctccaa ctcttctttt ttcaataacc gttacatctg
    16501 caccaagcat agaagctttt atagctgcta catatcctcc tggtccacca cctactacta
    16561 ctatcttcat ccttaattcc cccaaaccta atataatcta caatcttgca atactataaa
    16621 cctgcggcat ccagtatagc aggtatattc tcttctgccc caattgccat tatatgaaca
    16681 ccatcacata aatcttcttc ttttaattgt ttgataagtt cccctgccat ttttattcct
    16741 tcacttaccc agttttcttt tccagctgct cttaatcttt cgatttgttc atctggaacg
    16801 aatattcctg gtacatttgc agtcataaat ttagccattc ctggtgattt taaaggtact
    16861 attcctgcta ataccttgca atccatatcc cttgttaatt ctctaaactt tctcattgta
    16921 tttatgtcat aaagtgcttg tgtttggaaa aatttagccc ctgcttttat cttcttttgc
    16981 atttttaata attgaacttc tattggagaa tattctggag ttactgatgc tcccagataa
    17041 aaatctggag aaccttttaa cttattacca gccatgtccg tacctgccat taaagtctca
    17101 gcagtttgaa gtatccccac agaatctaaa tcaaacactc ccttagcctg tggatggtct
    17161 cctacgctag tatggtctcc tgttagtgcc aacaaattgt ttatcccaaa tacacctgct
    17221 gacaacattt ctccttgtat agctattcta tttctatctc tccctgttat ttgaataact
    17281 ggctctaatc cagcatcttt taataattta catgtagcaa gagaagttgc tctcataact
    17341 gccgattgaa agtctgttac atttgctgca tgaactcttc caactaacgg ctttgcacat
    17401 tctattaaat gtgaaaggtc agtccctttt ggaggagcca tttcagtagt aactgcaaat
    17461 tttccacttt ctaatgtttc tcttaataag ctcatctcta atcccccttt taaccttcta
    17521 aaaaaaactt ttatgaattt gctgctgctt ctttcttttt ctttgcactt aaacttcttg
    17581 gattattttg cttagaatag tcttttggtg gtcttatttc tgctaagtta tctaattgtc
    17641 ctatagcttc tagtctttca tatatcttta tccaagcaca atcattctct gagtttactt
    17701 cacacttacc atttttagct ccaccacaag ccccattcat aagaccttta gcacacatag
    17761 ttactggaca tatcccacct gtccaaccaa gttcacagtc tccacaagcc ttacaggctt
    17821 cttcatattc tccaactctt tgtacttccc ctataaacaa agtattgttg gctggataaa
    17881 ctggcttatc ttttaaattc ttaactattg tctgtgtacc atctccacat gctaaagata
    17941 aaactgcatc agcttcctta gtctcttctt ttagagcctt taaatccttt ttagacttta
    18001 aaaggttaca agcagggtca agcattacat atcctaagat ttttttgcct tcaccttcaa
    18061 gagtttcctt catttttaaa acttcttctt ccccaccact tttacaagtt gctgcacatt
    18121 gattacaacc aactaaaacc agcttgtcaa aatcctttaa gtaccctagc acttcttcta
    18181 atggtttatt ttcagaaatt atcatatcta cttcccccct aaaccttcca catatatatc
    18241 tctatttatt gttttgtaat ttacaagcct taactacatg ttttgcaagt atagatgttg
    18301 taactgaacc aacccccgct ggaactggag ttatcattga tactttgtct aaaactgcat
    18361 ttgtatcaac atctccacat aaatttcctt cttcatcaac atttatacca acgtctataa
    18421 ctaccgctcc atctttaaca aaactttcat caaccatctt agctctacca atagctgcta
    18481 ttagcacgtc tgcctctgct gctacacctg atagattttt agtttttgaa tgacatattg
    18541 taacagttgc atgttcactt aataaaagca ttgatactgg ctttccaact accattgatc
    18601 ttccaagaac tgttacctta cttcctttta aatcaacatt atagtgtttt aatatctcaa
    18661 ctactgctgt aggtgtacat ggtggaaatc cacttttatc cccttccatt accttagcag
    18721 agtttattgg actaaaacaa tctacgtctt tttccggtgc aataacatat tttataactt
    18781 cttcatttaa gtgttttgga agaggtctaa aacataatat accattaaca tttttgtcat
    18841 cattaactct ctttaataca tcaatatatt cctcttgtgt tatatcctca gctaactcta
    18901 acacttctgt tgttatacct atgttttgac atctctttag tgctcctctt tcataagata
    18961 aatcatcact tcttgcccca actctaacta ttgttagttt aggatttata ccttctttaa
    19021 ctagtaaatc gacttctttt attaattctt cactaatttt atcagctaca ggttttcctt
    19081 ttataatttg tcctttagtt gacattcctt ccatacattc acaactcccc tacaagcaaa
    19141 cttaaattaa tttattctat ataccacata ttaataacat agtatctact ttcgtcattt
    19201 aaaaattatt ttccaagagc tttttcaact ttggcataaa cttcatcaca aatttttaca
    19261 ccatcttcaa ctaacttatc tacttctatt tttaccttat taacatattc tttatcttgt
    19321 atagaattta tatttataac tacatttaat tgtccaccaa gtatagcagc tcttaaacac
    19381 tgaactccta caccaacatc acttatagca agtcttgaac ctttatcaac taaatcttca
    19441 tgtaacttaa tagcttcata gcaaactctt actatgttta atggtacttc acaagcaact
    19501 tttaatgcct tttccattgt ttctgctttt attctttttt cttcttctgt ttctttagga
    19561 agcccgtaag cttttgataa tggtaagaaa cattctgcat catcatctat catttttaat
    19621 aaatcttttt ctaactttcc tgccttactt aatatctctt ttacgctttc ttcatactca
    19681 gcatacttct tcttccctat tgtaaggtta cataccatac tacctaaagc catacctatt
    19741 gcacctacta gtgcagcagc tcccccacca cctggtactg cttctttgga agatagtact
    19801 tctacaaaat caacacaagt tttatctgct attttcatga taatcctcct tgaaaggatg
    19861 taggaaaata tctcaaatta ttcgagatat tttacgaact attttaagat atctcctact
    19921 ctttatttat aaattaattt ccttagaata atccagctat tactccattt tcatcaacat
    19981 ctattttttc tgctgctgga acttttggta atcctggcat cttcattatt tctccagtaa
    20041 gtgcaactat aaatccagct cctgctgaaa ttgttgcttg tcttacagtt attctaaatc
    20101 ctgttggtct acctaattta gtttggtcat cagttaaaga atattgagtc ttagccatac
    20161 atactggaac tttagtaaat ccaagttttt ctaagttagc tatttcctta tcagcttcag
    20221 gagtataatc tacaccatct gctccataaa tcttagtagc tatagcattt atcttgtctt
    20281 ttatagataa atcatcttca tagcagaatc taaagttatt ttcttcttca tccaataatc
    20341 ttaaaacttc tttagctact gcaattccac cttctccacc ttttgcccat acttcagaaa
    20401 gagcaacatt aacacctaat tccttacact tgtcttcaac aagtttaagt tcagcttcag
    20461 tatcaagtgg gaatctattg attgcaacaa ctgctggtaa tccatatact tgagttatgt
    20521 tttcaacatg ttttaataag tttggaagtc ctttttcaag agcttctagg ttttcgttat
    20581 ttaagtctgc tttagcaact ccaccattgt attttaatgc tcttacagta gcaactatta
    20641 taacagcatc tggttttaag tttgccattc tacattttat atctaagaat ttttctgctc
    20701 caaggtcagc accgaaacca gcctcagtta ttacatagtc tgcaaagtgc atagccattc
    20761 ttgtagctat aacagagtta catccatgag ctatatttgc gaatggtcca ccgtgtacaa
    20821 atgatggtgt tccttcaaga gtttgaacta ggtttggttt taaagcatct tttaataatg
    20881 cagccatagc accattagct tttaattgtc tagctgttac aggttcacct tcatagctgt
    20941 atccaactat tatgtttcct aatctttctt ttaaatcaga tatatcactt gccaaacaga
    21001 acgctgccat tatttcagaa gcaacagtta tatcgaatcc atcttgtcta actgctccat
    21061 cacctttttt acccatacca tcaacgatat ttctaagttg tctatcgttc atgtccacac
    21121 atcttctcca agttattttc ttagggtcta ttctaagtgc atttccttgg tgaatatggt
    21181 tatcaagcat agcagctaaa agattgttag cagctcctat agcgtgaaaa tctcctgtga
    21241 agtgtaagtt tatatcttcc ataggaacaa cttgtgcata tcctccacca gctgctccac
    21301 ctttaacacc aaatactggt cctagagatg gttctcttaa agcaactaat acatttttgt
    21361 ctagtttagc aaatgcatca gcaacaccta tagtagtagt agtttttcct tcacctgctg
    21421 gagttggatt tatagctgta gttagtatta acttagcttt tttaccagtc tctcttttta
    21481 gtagattgta gtcaactttt gctttgtatt taccataaag ttctacatca tcttcaccta
    21541 gtcctaattt ttttgcaacc tctctaatat cttgaggctt agcttcttga gctatttcaa
    21601 tgtcagattt aaatcccata actgaatctc ctcccacaac ctaaatagat ttaaaatata
    21661 atttttgtaa aaattgtatt aatatgtaaa aattatattt tataagataa ttatacatgt
    21721 aaaattactt aaaaactagc tactaagcta ttaaaatatt caaacttaat agctagttat
    21781 ttatctaatt acttacttat tacctatcta ttgcctatgt ctaaccttta accaacttaa
    21841 aatcccaaat caaaatttct atctatttta tttatcctta agtgacatta atttatcttt
    21901 tattgcttta atctgttctt tcgttgtttc actagaatca tatggagatt gattagctct
    21961 atcagaatca ataacatctt ggttatagta aataaatcct aaagattctc catcttctaa
    22021 gttttggatt ataaattctt catcttcctt atttctaatt ttatttccta ctacaaatac
    22081 tttatttaca cctatatcat gtccaagttt cttaactttt ctataagtct gtagacttct
    22141 ctctcctggt tctacaacta ctataaaagc atctacacct tgggctgttc ctcttcctaa
    22201 gtgttcaatt cccgcttcca tatccataac cactacatct ttattttgta gtattaaatg
    22261 tgagcagagt ctcttcaaaa gaacatgctc tggacataca catccagtac caccagaatc
    22321 tacggttccc aatgtaagta atcttactcc attgtattct ttacagaaat tttcagggat
    22381 atcgtctact ttaggattca tcttaaacat ttttccaaat gaacctacag aagcagctgt
    22441 tctatctgaa actaattttt tcatttctga tataggtact attgattcat atacttcttt
    22501 tggaaaacct agtgctaaag ctaaattagc atcagggtcg gcatcaacag caacaactct
    22561 gtatccgtct tcagcaaaca tcctagagag cattgaagaa aaagtcgttt tacctactcc
    22621 acctttacct gttattgcta tcttcataat ccctaccacc ttttattact atttgctttt
    22681 tagtagttaa ttttacagtt aaatattttt tatactctat atttttatac actaaaaata
    22741 aactacagct agtccttaca ggaaccaatt aaatttaccc tactagcttt tcaaagcagc
    22801 atgtatagtt ttattattct ttaatattgt ttatatttta tttattcagt atattcatta
    22861 tatttcgatg ttctttactt taatatttga ctttattcaa ctttactcaa ccagaaactt
    22921 cttataaaaa cgtttattct ataacttcac tattttttaa gcttcagcct tttctgccat
    22981 tttttcttca actaatgctt caaagtgaac acgttttttc tctatatctt ctattattgt
    23041 agctgctaac tcttttggat ttgtatttac agtaaatgta gctccaactt tatcttttaa
    23101 ctcactagtt aatatatcca ctgctcttga agaaccagct attggaggca ttattcctaa
    23161 ataagtgtct ataccagaag ctacaacata gcatcctata gcaactgctt tttctgacat
    23221 ccattctgga gctataccta caactggaag gtcacacata tccatatcta agtaattagc
    23281 tgctgctcca actaaatcta gtatacgact tatatcaaca caagaaccca tatgtagtac
    23341 tggtgggatt cctactaatt tacagacagt tgcaagacct ttacctgcat atttttcagc
    23401 cgcatcagat tccattaaac caaacttagc tgctgctgaa gctccacaac ctgtagtaac
    23461 tactattatg tcgtttttga ttaattcttt cattattgtt acgtgagcct catttgaaac
    23521 tcctttagca ttgttacatc caactacacc agctgctcct cttaatactc cagcttttag
    23581 acagtctgtt aatggcttaa ctgttcctgc tgggtctata tgagagttta caactctgtc
    23641 taattgattt actactgctt ctacactata cccaactgtt gcgcctgatt ttaattcagg
    23701 tataagtact tttgatttat ctctattttt aaagtttaaa atagcttctt taactattgc
    23761 tttagcatca tcaagtgctt gttcttctct aaattcaata taagttgaac ctgttatctt
    23821 agcttttgga gaagttgtta caaatttagt atggtaacaa tctgcaactc tagctaatgc
    23881 agggaatata cattgaacgt caactattac agcttcaact gctccagtta ttattgcaag
    23941 ttcttgttga tggaagtctc ctgctatttt tacaccatgt ctcattgtaa tctcattacc
    24001 tgtacaacac atacctgcta agtttattcc atcagcacca acttctttag ctaaagctac
    24061 taagtctggc tcttctgatg ctaaaactat catttctgat aatgctggct catgtccatg
    24121 taatattatg ttaactttat tttcttctaa tactgctaaa ttagcctctg ttcttcttgg
    24181 cactggagtt ccaaataaga tatcgcttag acgagtacct atcatagaac caccccaacc
    24241 atcagccata gaagttctaa gactcatgtg tattaaacta tctatatcac cagtacatcc
    24301 tatatgagta gagtgcatta ttgttgcaat ttctctatct atagctcttg gctctatagc
    24361 gtattctttc catatttttt gtcttggttc tggtgcattt tttataaatc ttaatgtccc
    24421 aaaaggtttt ccaaattcca ttaatgcaat ttctgccact tcatgtgcta catcatagat
    24481 atctctacct tctgtctcta catcccattc tttagctaaa gtaattaact tagcttcgtc
    24541 tcttatttta tagtttccat ttggatcagc taatgctaat gtatgtgcta tatctctagc
    24601 atggtctgaa tgggcagctg tcccaccagc aaccattctt gcaaagtttc tacctactat
    24661 agtatgctca tctgctccac ataaccccct tggagtctta gggcttattc tacatggtcc
    24721 cattccacat attctacagc aaacaccttg aagaccaaaa ccacattgtg ccttaaagcc
    24781 ttcttttcta tcatacattg tttctacgcc ttcttctcta gctttagcta tcatcttctg
    24841 gctgtttaaa tcaattgtta acattttttc atccatattc ctatcccctt tttttgattt
    24901 ttaaaatccc ccaggtgact gacttcatgt cataaaataa taaaataaat gctttctaag
    24961 ttgattatat actaattttc agtgctttgc tttagattta aatatttttt taacaaaatt
    25021 agataataca taaaaatttt attttctaca cttaatttaa gttgatttta tcgcttgttt
    25081 ttggctattt gttaatatct aaacctactt ttttgatact tcagtttaat taaattatgt
    25141 tttgattttt ttaaatcaaa tttgaaattt ttctagtcat ttttatatta ttgcagattg
    25201 tttgtgtatt tattatcttt cactactgtt tggacttatt acagattttt actccctaaa
    25261 cttaattagt aaaaacttct tagaatagca ttttttggac taattttttt gaattttttt
    25321 atttttccta ctttaatatt aattatagct tcaaattgaa catttgtata tattcatttg
    25381 aagctataat tattttttat ataaaacttt tttaatacag cattaattta gacattttga
    25441 ctattttttc tactttaatt catttatttc aagcgattta tcctatttca tatttttagc
    25501 tacaatagcc ccatatgcaa tagaatataa tttagattca tgtgaatcag ctcttgacac
    25561 taaaactata ggagcttttg cacccataat tatacctgct gatttagagt ctgccatata
    25621 tgtaaaggct ttccctattc cattgccaac ctctattgtt ggaaccaata tgatatctac
    25681 atctccagat acttcacttt taaacccttt tatctcacta gcttcttttg aaacagctaa
    25741 atcaaaagcc agaggacctt ctacaattac attttcacca aattctcctt ctttacaagc
    25801 ttttgctaac atatctgcat ctacagttgc ttgcatctta ggatttactt tctctttagc
    25861 tgccaaacat gcaactttta cagtttccat tcctaaagct tttgcagctt ttatagaatt
    25921 ttttaatatc tttgcctttt gttcataatc aggagctata ttcatacctc catctgtagt
    25981 tataagtaat ttatggtatt tttctaactc atataccata acatgactta ataaactatc
    26041 agttctaaga ccatagtctt tatttaacac ttcttttaat atgacagatg tatctaataa
    26101 tcctttcatt acaaagtcgg cttctttact tgatactaat ttaactgcta tttctgcaca
    26161 ctctttgata tcatccttat ccactatttt gataccactt aaatcaaatc ctatttcttt
    26221 tgatatttct cttattttgc cttcatttcc aaccaatata ggagtaatta tatttctttc
    26281 tacagcatcc tttattgcca ttaaaacttc tttatcatga gctgctgcta cagagagtac
    26341 aattttctct cctcccttaa gttgttctaa aatatcatct aattttttta tcaaaagtaa
    26401 cccctccctt atatatattt aactaaataa taccatattt gctatacctt ttctcaattt
    26461 attttttatg ttataaagaa tttatatgac taaaagacag tttaatattt tattctggga
    26521 tttcatcaat agctcttaaa agctttgcag gaacaccacc tacaatagta ttttttgtta
    26581 caactgctcc agcagcgata attgcattat ctccaatagt aactcctgga actcttcata
    26641 caaaaaagag gcttatctct attttgagat agccccactt aaccgataaa attaaataca
    26701 acctaacaat atccttactt atttattcca agttctctta gacaagcaat agtaatttca
    26761 tcttgtggat ttaattcata agctctttct atatagtaag ttgcatcatc aatatcacca
    26821 ttatttaaat aagccattcc taaattgcaa agtatttctg ggtcttcctt tatcttagcc
    26881 gcttgttcaa aatactctat tgctttatct aaattattct tataagcctc acaaagcccc
    26941 aattccacta tagtatccac ctgattaggc tttattatca atatcttctc aagatacatc
    27001 ttagcctgct ctatttcgcc cataccctta tatccaagtg ctatcataaa cagcaagttc
    27061 caccaatcca tatgttcttc ttctaatgga agtaacttct ctaatccttc cttaaactta
    27121 ccttggaata caagtgtata tccttcttca tattgaactt taaagtccat cttgcctaga
    27181 ttctcttgta cctcagctac taaatcagca tctaacccta gccttaatgc ttcttcccaa
    27241 gtcagcttag tctttaaata ttgaccttga ttataataat ggtagcctaa gtgataataa
    27301 cctaatgcaa aattctcatc aacatcaatt accttttcaa gcttagccat ggcttcaagt
    27361 aagaatgcat tcattgcctt atcatccata tctttttgat actgtttagc tatctcttgg
    27421 caaacaatcg catagttata cattgcatct aaatcatctg gatacattgt tataaatgct
    27481 tttatatata ttaaagaatc tgtatgttca ccaatctcaa agtattttct cgacatatat
    27541 cccatatacg atttcaaatc caaatcaata tcttttgata aagcatttaa aaacttctca
    27601 tactctgcat tgtttttaaa cttactatct attccaatta tatatatcat tgcatctgct
    27661 atagacattg aatttaactt atcttgagca gtcttttctt ttatcccttt tactaatacc
    27721 tcatttttta taggtacatc taatccgcct tttggcactt tatagctttt taatttaaac
    27781 tctccaccat gttttatagt tataaaagct aattcttctg ctttttttag caaatatttt
    27841 tctatcttaa aatccatttt atcctcctaa atacttaaat ctctacttta tgagttttag
    27901 atttctcaaa tattttttta tcatcatact caaataagac tacattttct acttctctta
    27961 tttctgtatt tatatcattt tttaaatcca caacattaaa tctaaatggc tcaaaatgaa
    28021 catattttat tatatgttta gctggtttat caacaaaact aggtaaatat gttgataagt
    28081 tatcattatc ttgtaaaaat acatggcatc tattcttaaa atcatccaca tctagcttat
    28141 taaaaaactg tggtatattt gatgttttac ccagtgatat atagtcatat tttaatatat
    28201 cattgaatac atcattatct atgttgattt tttcattata gaaatccatc aatattttat
    28261 ataactgatt ctttccttgt gccaaatcaa agtagccatt ttcatcaaaa tacttggcaa
    28321 aaacctcaaa gaatttaaaa ggactttgtt tataaaatct gcctataata tatctcatag
    28381 ataacacaaa gtttttagaa ttgtagtatc tctccagtat atcttctata tcctttagct
    28441 tcaaagtttc ttcatagctt atataattat tgtataaaaa ctcatatgga gcataatcct
    28501 tatatttaaa accatgctca tctgcaactt ttctcatacc tgtcccttta atcatcttta
    28561 aaaagcctaa ttgcagatgc tctataccta gattaaatac atcattaaaa gaattttcaa
    28621 agctcttata atcttcataa ggcagtcctg caattaagtc taagtgctga tgtatatttc
    28681 tataagatgc tacagtttgt acaacatgag acaacttttt aaaatcatcc cttctaccta
    28741 ctgcatcaag tgttttttca ttcgttgact gaacccctat ttcaaattga aaaagacctt
    28801 ctttacaatc agccaaaaac tcaagcatct tatcatctat caaatacgct gtaacttcaa
    28861 aatgataagt tgtatatcca ttatcatttt tcataaggaa atccatgatt tccatagcca
    28921 cttttctatt tgcattgaat gtcctatcta taaactttat ctgtgatact cttgcatcta
    28981 ttaaagcttt taaatcactc tttatcctgt ctaaactaaa ataccttaga cctggaatag
    29041 tagatgataa acagtactgg cagttaaaag gacaacctct tgatgattca taatacacta
    29101 ttctattttc atattctttt ggattcaagt tttcataagg actaggaatc tcatccaaat
    29161 cttgaagtaa ttccataggc ttattcacta ttatctcatt gccttgtcta tatacaagtc
    29221 catttacatc atttattttc atctttcctt gcaagtgaag tactaagtct ctaaaaatta
    29281 attctccctc tccataaagt atataatcca caaaatcata ctttttcatt gcctcataac
    29341 tatcataact cacttccggt ccgcccaaag ctattttagt attaggtcta atttttttta
    29401 gattatcaca aagtttaact atatctccca cattccagat atatgttgag aataatatta
    29461 tatcatattc atttttatat atatccttta aaatatagtc caaatcgttg tttatcgtgt
    29521 attctttcat attaacttct atcaaatctc ttacaaattc ttttagatat cttatagcca
    29581 aatttgtatg aataaacttg gaatttaatg ttgttaataa aattttcaaa tatttctcac
    29641 ctctttttaa gattatactg tctttttgta ccccttatag gttaaaatag tatatgtaaa
    29701 gtctaaataa tatctatata attaatttat taatttcaag taatgtgagg aaaggaagtt
    29761 ttttatggaa ttaattgaaa aagtatctat caactcttta tcaaaaagag atttactact
    29821 tattataaag tctcttgaat acactaatga aaatactcaa attagtgatt tcattgacct
    29881 taggaataat atagtaaagg aattatgttt tttaactgac actaaggaaa aagatttttt
    29941 agattatcta gaaaaaaata gcacactcta ataacctaaa cctttttaaa ctttaatata
    30001 taaatacctg atttagaatt tatttttatg cttaatatat tatcattaaa ggttttcgac
    30061 ataactgtta tttgattaaa tatagggtct attatagtat taccttcttc aagtcttata
    30121 agacttcttg ggtctatgct tttattatat gccttaactc caattttata ttttctcaat
    30181 ttaacaacat attcggacat agaaaaaata aaatctatat tatcttcatt taatttacta
    30241 tcttcaccca aaatggataa tatagatttt tttatgctat taattccttc attttctaca
    30301 gttctatctt ccatatatac atcatataga acatttaaaa catttaataa aatactttct
    30361 tcatcataat cttcagattt caaatcaatg tacttatcaa ttaattgtat tgctttaatc
    30421 cttgccatct gaaactttac aacatctgat ttgtctaatt ctatagaaaa tcctattatc
    30481 ttgtctttaa tactctgtag taattcgaca tactctattt tactattatt tataagattg
    30541 tgcataatgg aattatacac tacagaacct aataaataat caaataaaag ttcttctttt
    30601 ttaaaataaa taacatactt ttttattagt ttaataacct catcttcgct tttttgaata
    30661 ttagtatttg ataaaattag tggtagtaaa atatttaaaa aattgatatt tcctattttt
    30721 tctccataaa atctatagta aataagttca tctctcagtc tattttcgtc tatatacaca
    30781 catctttgcg ttttatcatc tattaaggat tttgtattta ttatagttaa atctttaaga
    30841 gccctacttg cactactaaa agaagatgtg tagacttttt ttagtaattc gtcattcata
    30901 aatatatcct ttctgaaaaa ttgaatgtaa ataaattgtt attaaaaaaa aagagacagc
    30961 ccgtttaaga agctgtcata tataggggga tagtagtgta caaaaatcat tgtaatctac
    31021 tatctatata ttattatacg atactacttt tttatatgta aagcatcagt tttcgacatc
    31081 cctatatttt acagttatac aaattgcgta ctaagttcaa ttttattctt tttatttcaa
    31141 cattttatca tgctacattc tttccggagc gtgcattccc aacaatttca gagaattttc
    31201 taaagtttgt tttgttgcta aaactaatgc aagtcttgct tttcttattt ccaaattttc
    31261 tactaaaatt ggattgtcat gataaaattt attaaacgct tgagctaaat ctaacataaa
    31321 tctagtcact atatgtggct catttttctt tagagcaagt aaaatattct tattgaatga
    31381 ttctataact ctaagaactt ctgcactatc ttcatcagct aatagactat aatctatatc
    31441 agaagtcact tctacttctg cttttcttaa aactgcacag catctagcat gtgtatactg
    31501 aacatatggt ccagtctcac catcaaaact aagcgttctt tcccaagaga aggtatagtc
    31561 tttaattcta ctgttagata actcttggaa tactactgcc ccaacaccta cttgcttaga
    31621 tatttcatcc acatttttag catttggatt tttagctagt ataatttcct tagttttatc
    31681 tatagcttga tttaaagcat cttctaaaaa tactactctt ccttttctag tcgacatagt
    31741 accttcttca agtgcaacca taccaaatcc aacatgaatt aaatcttttg cccaatcata
    31801 tcccataagc tcaattacct taaaccattg ttggaaatgt aagttttgtt gagaaccaac
    31861 tacatatata cacttgtcaa aatcataagt ctcttttcta tatattgcag cagctaaatc
    31921 tcttgtcata tataacgttg aaccatcatt cttagtaata agtgctggtg gcattttata
    31981 ctcttctaaa tcaacaattc ttgctccttt agactctttt aaaagatttt tctcttctaa
    32041 tagttcgata actctgttca ttttatcaga ataaaaactc tctcctgcta aagaatcaaa
    32101 ttctatatct aaaagtttat acactctgtt aaactctttt aaactctcat ttctaaacca
    32161 ttgccataat tcttgtgctt cttcgtcacc attttctaat ttattaaacc atgctcttgc
    32221 ttcatcttcc atttctgggt gttgttcagc ttcatcatgg aatcttacgt ataattttag
    32281 taattctgga attggattag actctactac ttctttttct ccccactttt taaaggccac
    32341 tattaactta ccaaactgag ttccatagtc acctaaatga tttattctta tagtcttgta
    32401 tccttgcgaa tcatatatct tatataaagc attacctata actgtagttc ttatgtgccc
    32461 tatatggaaa ggttttgcta tatttggaga agaatattca actattacag ttttatcttt
    32521 tccaaaatca ctatgtccgt aattttcttt ttcatctaaa acttttttaa ttacagtttc
    32581 tgctaattga gatttattta caaagaaatt tacatatccg cctaattgta ttacttttgt
    32641 tatctctcca cttggttcta tactttccgc caattcactt gctatcatgt ttggagcttt
    32701 tctaaacact ttggctaatt taaagcatgg aaaagcatag tcccccatat ccttatttgg
    32761 tggaacttct ataagtgcct ctatctcttc tttcgataaa tcttctattt tttctttaag
    32821 gcaatttgat attgccacct taaaatcttg cattctgttg ctcctctcta cattgttcct
    32881 ggtttatatt gtgcttatct ctttatgtta aatcaaattt cgtgtttttt caagtatttc
    32941 attacagtgt aaaacacttt aataattatg ttttctgtat atattaaaaa tactataatt
    33001 gcagttttta atcacactat tattgatttt ttataagact tttttctatt ttattgtttc
    33061 ttgtactatc cagttgactt tatatacttt ttttaatctt ttatcaattt tcatacatca
    33121 atattaattt tatatacatt tattttgctc ttttatctat ttttatacat caatattagc
    33181 tttatatact ttttttactc ttttatctat ttttatacat caatattagc tttatatact
    33241 ttttttactc ttttatctat tttctataaa tctttttcac tcttaatttt tattccttct
    33301 tcttctaaca gccttgctgt tatacctttc cctggtatct ttttacctga aaaactacca
    33361 tcatatatta gattacatcc acaagagggg ctaccctctt ttagtattgc ttcgctacat
    33421 acatataaat ttgctatttt tagagtttca ttagcccctc ttacaaagtt ttctgttaca
    33481 tccttattat ctttagtaat tacttttcca tcaactatct cagcaggtgg tcttggtgta
    33541 gatagaccac ctaactgctc tgggcataca agtgtaaatt ctttgccttt taaatattcc
    33601 ttgacttttt cattttcatt attatcgcca ctatatttac aatttattcc tattaaacat
    33661 gcagatacca atatcataaa aatgtgctcc tatacaaatt cttaatatat tcttatacaa
    33721 attactaatg tattcttata caaattattt taatttaaca attgttactc ctgctccacc
    33781 ttctccatat tgaccacctc gttgtgattt tacatgtcta tttcttctta aaatgtcttg
    33841 tagccctgct tttaatactc ctgtgcctat tccatgtatt actgttacag actctaaacc
    33901 tgccacatat gcatcatcaa gatacttttc tacttccatt atagcttctt ctaggtttaa
    33961 ccctctaagg tcaacttcat tttttacact accagatttt gaccttataa tgtttctcgt
    34021 tgatttagtt acatttgttg aaacatcttt gcgtgttttt tgtaaagatt taaaaggtag
    34081 agtcatcttc attataccaa tttgcactac tgcttctttt ctctttttat ctactgaaac
    34141 tacactacca ttttgattta aagttattac cttaacttct tcacctggct ttaagtcttt
    34201 tatctcctta tttgatacct ttggaactat catacttttt actgttggct gaagacttcc
    34261 catagagctg gtcaattctt ttctaagttc ttctatctta cgattctttt cttttgaagc
    34321 tctttcttgt tcaagatttc taagttcttt tattataata tctacttctt ctttagcttg
    34381 tcttataatt gaaaatgctt ctgatttagc tttttcaatc atcttatctc tttgactgac
    34441 caatttttca agtttttcgt catactctac tttcagtttt tcaatctctt cttttaatct
    34501 ttcagcttct tctctatctt ctactgcttt gattctattc ttttcaacat tctgtaacac
    34561 atcttctaaa gctatgtttt ctgtatttat atattcttta gcacgtgaaa tcacataatc
    34621 acttagccca agttttctag aaatctcaaa cgcatttgat tttccgggaa cacctataag
    34681 taacttatat gttgggctta aagtttctat atcaaattca actgctgcat tttctacacc
    34741 tggtttagta agagcataat ttttaagctc actatagtga gtggtagcta tacatttagc
    34801 tcctacagaa tttatatctt ctaatactgc tattgcaagg gcagctcctt caacagggtc
    34861 tgttcctgct cctaactcgt caaatataac taaagagtct gctgttacgt tttgtaatat
    34921 agatactata ttcgtcatat gagatgaaaa tgttgataga ctctgttcaa tactctgttc
    34981 atctcctata tctgcaaata cattatcata tacacacata ctacttccat aatcagcagg
    35041 tatatgaagt ccactttgtg tcataagtgc aaatagacct acagttttta ttgtaacagt
    35101 cttaccacct gtgtttggac ctgttatcac caatgtatgg aagtctcttc ctaaataaat
    35161 tgtatttgct actaccttct ttttatccag cagtggatgt cttccatttt ttatgtttaa
    35221 atatttatcc tcatttaaag ttggttctat tcctctcatc tgtatagaaa gttttccctt
    35281 tgaaaatgca aagtccaatc ttccaagtat ctcttgatta gagattaaat cttcgctcac
    35341 ttctccaacc atagctgaaa tctctgataa aattctttct atttcctctt gctctcctag
    35401 tttcaactgc ctcaattcat tgttcatctc tactatagtc attggctcta taaaaagtgt
    35461 cgcaccagaa gatgactggt catgcactat accagctact tgcgacctat attcagattt
    35521 tacaggtact acaaatctat cacctcttag agaaattatt gcatcttgta agtacttttg
    35581 ataagtagtt gaacttatta ttgaatttaa ttttgaccta atggattgat ttttttgggc
    35641 tattcttctt ctgatatctc taagtgtact tgaagcattg tcagatattt ctacttcact
    35701 aattatagca ttgtatatct ggtcttctat gtccttgtac acatatagag aatttgatag
    35761 agattgtatt attggataat taaaatcttc ttcatcacta ctagataagc tatttcttaa
    35821 tcttctagcc actcttaagg tatctgctat cataatcaga cttcctgggt caagagaagc
    35881 acctatataa gctctcttta ccttatcttc tatatcatga ataccttcca accctactga
    35941 acctctcttt ataataattg attgagcttc actagtttct tctagcatag atttcacttc
    36001 tacaaaatca gtatttggta ctaaattttc tatatatttt agacctaaag aagatgaggc
    36061 tttcttcttt aacaaatcta ttattttatt gtattctaga actcttaacg atttctcatt
    36121 cattttctat attcaccatc ctcgatattt taactttttt atttaatttt aaaaatgtat
    36181 gttttcaagc atttccaata aaaaatgcaa gttttatttc tttttcattt ctaaaattat
    36241 tatatttttt ctcttttaga tatattttac acatattttg tattatacta tgtatgtagc
    36301 tatctatata ttatacataa acatgcttta tttgtaatat aatctgttaa aaaatcttct
    36361 ttaatatttt taaaggagtt tattttagct tataaaaaaa tagctactta tatagataaa
    36421 ttaaggagga acatgagtca tgggaaacgt attagaagga ttaaaaccag aaagtgtttt
    36481 taaaaacttt gagaaaatat ctcaaattcc tagaggttct ggaaatgaaa aaggcataag
    36541 tgatttttta cttagctttg gtaaaaattt aggtcttgaa actattcagg atgaaagctt
    36601 aaacataata ataagaaagc ctgctacaaa aggatatgaa aattgtccag gtgtagttct
    36661 tcaaggacat atggatatgg tttgcgaaaa agaaaagaat gttgaacatg attttcttaa
    36721 agaccctata aaattaagaa ttgatggtga catgatatat gctactggaa ctactctagg
    36781 agctgacaat ggtatagctg ttgctatggg tatggctata ttagaagata atactttaga
    36841 gcatcctgca ctagaagttt tagttactgt taatgaagaa gatggtatga atggtgcaga
    36901 tgcactagac cctagcttaa ttaaaggtca atatatccta aatatggact ccgaggagga
    36961 aggatactta ttagttagct gtgctggtgg taaaacttgt gttgttagtt tacctgttga
    37021 atacaaggaa gttaaaggtg acaaacaagg attattagtt gaggttactg gactattagg
    37081 tggacactct ggtatggaaa ttgttttaca aagagcaaat gctaacaaag caatagctag
    37141 agtattaagt gtattgaatg ttgattatga attagcttct gttgatggtg gtactaagca
    37201 caatgctata cctcgtgaag ctaaatgtgt aatagctgtt aataaagctg atgttgaatc
    37261 tgctaaaaaa caaataaatg acattttaac tgcatttaaa catgagttta ctacttctga
    37321 ccctggcatg acttactcag tagctgaaac ttctgtagac aaagttctta ctaaagattg
    37381 taaagaaaaa gttgttcaaa tgtcttgctt aactcctcat ggagttcaat cagtaagtct
    37441 tgatatagaa ggattagttg aaagttctac taacttcgct ataattgaaa ctaaagaatc
    37501 tactattgaa tttttaactt ctgttagaag ttctgttatg tctattagag atgaaatcgc
    37561 tgatagaata agacttttgg ctcaagcttt aggtgctaat tacgacctta tagctcaata
    37621 cccagcttgg gaatttaaaa aaggctcaaa attagaaaaa atatgcagtg aaacttatga
    37681 gaaattaact ggtaaagttc ctactgttat ggctctacat gctggtcttg agtgtggatt
    37741 attacttgat aaacttcctc atgctgaagc tatctctata ggaccagata tgtttgatgt
    37801 tcatactcca aatgaacatg ttagtatccc ttctgtagct aatgtttggg attatgttat
    37861 tgaaatatta aaatctatga accaatatta aatttaagtt ttgattctaa atagatttta
    37921 attttaagta atctatataa aattaactct gtaaattaat actactgtta gtataatatt
    37981 tactttcaat attaacttat agaaataaag cctaatgata gataataaca tgtatcatta
    38041 ggctttattg taatttaaat attattaaac tttattctgt tttaaaatta attcttatac
    38101 tattttaaat ctatttcttc cttgtctctt agaatcataa agagcattat ctgctatttg
    38161 atacagttca tcatactctt gaccacgctt tgttataact acccccatac taatagataa
    38221 actttctcca tttggcaact taatgagatg aatatgattg tatacatcat tacatttatt
    38281 gataagttct tccttattta ttatatcatt tatataaaca ctaaattcat ctccacctaa
    38341 acgacctact atatcatctt ttgcaaaact ttcatcaagt acctttgcaa catcaatcag
    38401 tactttatct cctaccaggt gaccataagt atcattaatt gacttaaaat aatcaatatc
    38461 tataatcaat aaagctccat ttttttcagt tgataaattt aataaatttt gtgtagttaa
    38521 ttttctaata tatgctgcat tataaatatt tgttaaacta tccctttgaa ctttatccaa
    38581 caatctattc ttttcctctt tttcagaatc aatattagct attttaccaa ttacatacat
    38641 tggtattttt ctatcattat aaattatttt tgaagtaatt ctaatccaat gaaaagtatc
    38701 atcaagcatg atagaataca tttcagtagt cccattttct tgtgatttta ttacatcaaa
    38761 aaacttttta ttatattctc ttcttatttt atctctttca ttttctatat tgccattttc
    38821 actttcctta tcacttttat ccaaaccata ataagtctca ataccctttt ctggcataga
    38881 aattatcaat ttatcttgct tataatcata ctcaaaaaag tattcacttg tcatttcata
    38941 caactgagta tgttttttga gttccaaaga tatcttttcg ttcatacttg tacgagtatg
    39001 taaaccaaat gccataaatc caataataca aattaataaa cttgctacaa taaaaatagt
    39061 ctcaattgga ttctgttgca taatataaag caacgagaaa tcttgtctat atgtagtatt
    39121 ttggtagaca attgattgca ttttttctga tgaaatactt ataatagctt tattaaatat
    39181 attcaataat tcttgtttat cttttttaat tatgccaaaa caaatactct ttgcttcata
    39241 agtttgagga actaggcgca catttttata cttaggttga ttcatatagt actgcataga
    39301 ataaccttct ccataagtgt aatctgcata gcctttatta acagcttcta tacactcttc
    39361 taaattatca taccaaacta tattttcttc aaactgacca taataatcaa tacctttggc
    39421 tagagctata cgctttccat ttagactatt ctcatttaat ttattattaa tcatcatagt
    39481 atattgagta gaaagaaaag gccgagacat agctatatta tgttcacgag agtactcata
    39541 atcataaatc attcctgcta caatatcaat ttcatgtttt ttagccatat cttccaaatc
    39601 ttcttgagat tttacaggca caagttcaaa ttccaaacct gttttatcac atatatgatt
    39661 taacaaatca attgatatac ctcttaattc tcctgtatct ttatctgcat tttgaaacgg
    39721 tggtttgttt aaaagtactc ctacttttaa tttttctgta tctttaatgt aattagtttc
    39781 ttcatcagat aaaaacattt gattattctc tggcgtaaag tatttctcat ataaagtagt
    39841 tgaaaaatat gggtccttct gttcaatatt attaattgct gaattaagct tttgagttaa
    39901 gtctgtactg ccttttggag taatgaaata aaatggcttt gatgagaatt ttgctacagt
    39961 acgcacacct tctattgggt tcatactaac attaagaagt acatctgctt taccactctt
    40021 tagtacttct aattgctcgt cacttgtttt acaatatacc aattcaggtg acataagatt
    40081 cattttacaa tactcatcta attctttaag cctagtatca gaattttcta ttacagcaat
    40141 acgaattttt ttcacatctt gcgaactcat tatgttatca atatctgtat tttcatataa
    40201 tgcttgtagt actgtatttg ctactccata actgtttccc gcataattat atattttttg
    40261 cattggctca ttgtataaca tacctcccat tatatcaatt tcaccatttt ctaacatctt
    40321 cattaatttt gtaatagatt catttatctc tccaggaact tgaacaaatt cataattcca
    40381 tccagtatac tgtgcaattt ctagcaaata ttcatatgta tacccaaaat agttaccttg
    40441 ctcatctatt tcagttaaac ctttttgaat aggataagca acacgaatgg tacgattatc
    40501 attacctata gcattgatac caaaagaaaa tatgcaagaa gtgaatatag atgtaattac
    40561 aataattaat aaaagtaatt tttttaaatt cactacacaa tcctccatat agttaagatt
    40621 tacagtataa attactgcat aaacttgtag acatgaaaaa atagattagt atataaaagg
    40681 atatataaat ctacttagaa atttaaatat ccttaatata tacaatatat ttaaattata
    40741 actcatttat atatctatta caacaaattt accctgctaa atacctactc tatatcccat
    40801 aattttacaa gttgtaatga agtttaagtt ctatctcatt tcaaatttag cagtgtttct
    40861 atttacagtt cttttatatt tcacatctgg ataacccaat ataaatgaag tgataactgc
    40921 ttcgttctcg ctcattccta acagttcttt tatctttggc tctacatttg atgccataac
    40981 aaaaaatcca ttataacaaa ttcctaatcc ttgagaatag gccataagtt ccatattaga
    41041 tgcagccaat cctccatcta cataagcact tccacctaga gacatatcac ctacaactat
    41101 cataagtagt ggggcatcat aaaataaaga atcatttccg ttctctttat atcttttgta
    41161 cattttctta aatgtatttt tatagataga acgtacaggg tcattgtcat cagtatctaa
    41221 tgccaaatta taaagacctt gtattgccag ttctttcact tcttttagct tttcttttac
    41281 taatatgtat cttataggct gtctattacc acctgttggt gtgtatctac cagcttccac
    41341 tatgtttttt attttttctt cttctacctc tatatcttta tattgtctta tacttcttct
    41401 aaatttaata aaattcaata acttttcggg ttctaattca aaactttcac gattgtattc
    41461 tattacttca tttttatcaa atccttgcat ttctattgca tctgttgggc aaattgccat
    41521 acagtgacca cagttaatac agagaatatt tttaaccttt gctttatcat ccttaacttt
    41581 tattgcattt acaagacagt ctgattcaca catcccacag cctacacatt tttctaaatt
    41641 tatgtctatc attccaaatt attcctcctt ttaaaaaatt tactttagta taaaacaaat
    41701 agttgtctaa gtcaattttt atttttttaa caatatacta gccttaacta cactttaatg
    41761 gagaaaaata tacataaaat tagaacttaa attagaattt attcaaaata aaagataaaa
    41821 gaagtaaatg tctaaaagtg aactttttag ttaatgagac attctttatt gtatgaaatc
    41881 aagtggatat ttatcatttc aacaataaaa aaggcttacc tctattttgg atatgtcctt
    41941 gttaagtaga cagactaaaa aaggctatct ttatgcactg agatagcccc ttttcccaac
    42001 tactattgtt tattttaata ttatatcaat aaatttaatt tgctataacc tgtaagttac
    42061 attttaattt gatgtaactt taaattacat ttttaagtaa tatctttaaa ttatattctt
    42121 aaaatatatc attaaactac attttaatta atctaaaaac tatatcttaa ttaatctaag
    42181 aaacttacat tataatcttt tagtttccca ttatcatata caatctttgc actaaagata
    42241 tttccagtct tagcccaatt taagaacaac ctgtcacctt cccacatatt taaatcgaat
    42301 attttatcat taccaaccca aattaaattt ccttctttac aatcatttaa atctttttca
    42361 gacaattcac cttcaaactc atctgagaaa tacaaaaaca tataccaatc ttcttcatct
    42421 ttaaattttg gaaaagttat caacccttta tatgacaatg atttaagagt aagaccagtc
    42481 tcttccttta cttccctaat tacacattct tctggagtct ctccttgctc aaactttcct
    42541 ccaactccta catacttacc ttcatgtata tcatctttct ttttatttct gtacaacatc
    42601 aaagtcttgt catctttttc tatataacaa atagttgtaa gtttcatgta tctcgcctta
    42661 ctttctcttt atattctcaa ttaattattt tcagcacttt caagcacatc acttatattc
    42721 ttcattgcat taagagaaag ttctgcaaat tcatcaaatg gaatccctat atcttctata
    42781 cttctcattg catctctatt tacatttgaa gcaaatctcg tttctttcat tcttttaata
    42841 acagacttag tctttacact agtaagtttt ttatctggat aaactaaagc tatagcagtt
    42901 ataaacccag ttatagggtc tgctgcatat attgcttttt ccattaagct agttcttgta
    42961 actcccattc catcatgatg accaagtatt gctctataca tttcttcgtc accgaagttt
    43021 tcttttttaa gcagttctac tgttgttgta gcatgtcctt cacatatttc tcctggcttt
    43081 cttccaacta tatctgaatc caaatcatgt aaaagacctg ccactgccca ctcttcttct
    43141 ttttctggct ctagtctttt ggctaattct ctcataacag cttcaacagc ataacaatgt
    43201 tttctcattc tatcagtttc taaatactta aataaaattt ctaaagcttc ttctctcatt
    43261 ttcaccaatc cccctgtttt ttcacatata tgatattttt atatattaat acacataaat
    43321 tcaagtcttt atatatattc atgtatattt ttcatgttat atctattcta cacctttata
    43381 gtagtgtcct gcaactgttc caatttcatc taatttctta tacaaatctg tagccttttt
    43441 tccaagttta taattattac tcaatacctc aatgtgagct tcaataacct ttttaaccat
    43501 ctcactatct tcaactgtaa aatctatcct aaatacatct attccaattt cctctaactc
    43561 atatagattg tccaacatac aagtaacctt cgaattgtaa attgtacttc tacagaatat
    43621 atcttgtgtt atcctataac ttgcatccgt aaagtctctt aaagcataac tactttcctt
    43681 acatttagca actctcttgt cctttttaca atctctaaca actactccca taggacagta
    43741 ttctgtaacc atcataggag catatccata tattactgct tcaatttcta catctgtata
    43801 ttttaaagtt tgtttaattt catttaagtt tagttcttgt gacaaacaaa ctgtacttgc
    43861 tccctcttct ttgaagtggt ttatactttc actattgaat gcatttaagt agcaatctat
    43921 atttaagtct tttttcttaa agtattccat agagccccag ttaccaattt gaaccttatt
    43981 aattcctttt atattatcca atatctcaaa ttgcttgtac tctttatttc taacaatcct
    44041 tggtgctgaa tagattaatt ttttgtcagt gactgctgta ttatagtggt tcttattcaa
    44101 agtatctatt ttacttgtat ttgacaaagt atctatgtta tttgtatttg aacccattag
    44161 ttctttagga atacctaaag acgaagaagt atctaaatcc atagaaatac ttgaatctat
    44221 agaaatactt gaatctatag aaatacttga atctatagaa atacttgagg ccatttgtaa
    44281 agcttttgat aatgtattta tatcttcata atagattaaa tctacattat aatttaatac
    44341 tgcttctaat tgctccaagt tcttaacttt aactctaatc ttaggaactg tatcaagttt
    44401 tttctgttta tttactctat ttggttggta ttttaatcta ttcttcttaa attttctatc
    44461 ttttatcttt attctttctt catctaaaag ctcaatacaa tcccttctca tttgatttaa
    44521 cacacttata ggcatactta caccttcatc caggtcaatt tctatctctt ttaacttata
    44581 tggtgtattt ccaagctttt gaatttgtgt ttctattttt tctttactta aagccacctt
    44641 tatagctttt tcaactattt tgtctccttc taccaccgat ttatttccac aaaaatcact
    44701 tataaaaagc tctggctttt tacctagttt aatactcact tttgcacgta tattagtttt
    44761 tatgaactct ttatcttgct gaaatgtagc ttgtactcta ttcattaatt cgctgtcaga
    44821 agtcttatat ataagctgat ttctctttgc ttctccaaca aaatctaatt ctatcaaatc
    44881 ccctttatgt gctattgtag ttatctctcc atcctttatt attcttccaa tagtacctcc
    44941 acctaagtta attccatcac ctttcttaag cgtattttct agcattactc taagtctttt
    45001 gttctttcta ttataatcta aaacttttcc aatatatagc ccttgattat taggtctatt
    45061 agagttcatt atatccttac caacttcacc taatatatat ccttttgtaa actttctatt
    45121 aaatatggta tacaaattat ttattatttc ctcagaaata cttatttttt tcttatctac
    45181 aaattcatta atcgcttctc tatatcctga aaccacagta gctacatact ctggtttttt
    45241 cattctccct tcaattttta aagataatac acctgtttca attattttat ccaaatcttc
    45301 tatagtactt aaatctctag gactcaacaa ataatctcca ttacttttta ctacttctcc
    45361 tgtatttata tctatcaact catacttttg tctacatggc tgtgcacacc tacctctatt
    45421 tccagaccta ttgcctatca tactactcat aaggcattga cccgagtaac atacacataa
    45481 agctccatgt acaaaaactt ctatatctac agttgtattt tcacaaatcc attttatctc
    45541 atcaacattt aattctcttg ctaaaacaac tctatcaaac cctacactct gaaggtattt
    45601 tacatcttct aaggaatgtg ctaccatttg agtgcttgca tgtagttcaa agtcaggtaa
    45661 tagttttttt ataagtcttg ccattcctat atcctgtact attaaagcat caatatctat
    45721 atcgtataaa aattttacat actctacaaa atcttctatt tcatactgtt ttatcaatgt
    45781 atttacagta acaaagactt gcactcccct tatatgagag tatttaacag cctcaatcag
    45841 ctcatttcta tcaaagttat ttgcagatgc tctagcacta aagtctttac cacctaaata
    45901 cacagcattt gctccgtttt gaactgctgc ttttaatgaa tcaaaagagc ctactggtgc
    45961 aagtaattct atttttttca tatttttctc ctctatttat acttgttagg ttaatataat
    46021 tatatctata acttaaatat ttgtattaaa tttatatatt tcttactata ttttaaattc
    46081 ttcaattaat ttatatatta gttatttata tttggtccat ttgagttaac atatacatta
    46141 tttactatat ttagtatatt tctattaact tatatattga ttactgtatt ttaaattctt
    46201 caattaattt atatactgct tactaaattt aaaacatttt aattaatttg tacactgttt
    46261 actatacttg aaatacttct attaatctat atattgccta ctatacttaa gacattttaa
    46321 ttaatttata cactgcttac tatacttaaa atacttctat taacttatat attgctta
//
LOCUS       DAELSY010000012        45259 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.012, whole genome shotgun sequence.
ACCESSION   DAELSY010000012 DAELSY010000000
VERSION     DAELSY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45259
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.012"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(353..811)
                     /locus_tag="KPG09_000574"
     CDS             complement(353..811)
                     /locus_tag="KPG09_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0965202.1"
                     /translation="MDVNEVEIIDSSYEKGKELTEDQCNMVTLLITGATVTETARIIG
                     VNRKTIYNWMNKEHVRKEMDRRKQELTNQGNLMILKDLDSYINNIKELASDKSDKRVM
                     LAANQYLINRIYGTPTSTVIQAEDNSVGMGMDATEIEAAIAKIRIRTSKK"
     gene            complement(1026..1670)
                     /locus_tag="KPG09_000575"
     CDS             complement(1026..1670)
                     /locus_tag="KPG09_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0965203.1"
                     /translation="MEAHSGQKVNPIKDIEDVFRLLNFLEEWNERNYLLALFGMCTGL
                     RIGDILALKVADVTDIKLDKKGKKIRVSKDWIRVIEEKRDYDREVFLSDVIKSAIENY
                     TQDKPGEEFLFKSGKRKKFNRPIQTRQAGRIIKGAAEKVGIKENVATHSLRKTFARHI
                     YDEEENKTYALELIRKILGHKTIEMTRRYIGIDKDEEVKAITKFTNKLKKRKRN"
     gene            complement(1674..2219)
                     /locus_tag="KPG09_000576"
     CDS             complement(1674..2219)
                     /locus_tag="KPG09_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965204.1"
                     /translation="MAWNLRSKSFLLSREVLKIKRDITEEISSAAYVPDNLKYYDSVM
                     RETHSMYGRECSLDFIKKKQEKLLKLKKKIKKNYDSNINKIDSYLKILEESIIDESDH
                     VELVVFKLYLQLENVVKVTETVNDLGFRIKTNTYARERKYSTNDITSIITDPFANVVE
                     DLKILVQDRQRKNYHGNKECF"
     gene            complement(2363..2806)
                     /locus_tag="KPG09_000577"
     CDS             complement(2363..2806)
                     /locus_tag="KPG09_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HBG0965205.1"
                     /translation="MQSSKKDNLFSSAEGKLYNYKKIKAEIEKIDIDMQMIKNDYRGC
                     SGVEIKEKTSKTYDIKSVIEIETEEKEKKIALKEKEKRHKELIIEKIDNAMKILSEEE
                     KKIVQYKYFSSSRTSWEYVGRMIGFSASKCKQMRFDIIDKIKGLL"
     gene            complement(2914..3171)
                     /locus_tag="KPG09_000578"
     CDS             complement(2914..3171)
                     /locus_tag="KPG09_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965206.1"
                     /translation="MLIEVTENNRELKVGDIVEINGGNKLYMILSLKETIGGYVIINM
                     QNGYGSFGAYKSLSQLELDLKVRGYKLYSSDDYKLQLVPKE"
     gene            complement(3191..3676)
                     /locus_tag="KPG09_000579"
     CDS             complement(3191..3676)
                     /locus_tag="KPG09_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965207.1"
                     /translation="MDLKLISSIPNWKVEDQLAKIDEEVTEFKEAIDTGMTKDIIAEG
                     LDVCQTILTMFQILELEDYIAEGIEIHNKKLNGRGWDLKEIGDGILNLIKNSMINELI
                     NMNNKLKCVDEKEFNSKIQFLDVNKLWDLCVPLVNLSAMKGINENIDEMKMGEFLKNI
                     N"
     gene            complement(3749..4078)
                     /locus_tag="KPG09_000580"
     CDS             complement(3749..4078)
                     /locus_tag="KPG09_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965208.1"
                     /translation="MSCVIKCDFCGETIAEILNIKVFRIDDEGNIMSVGKDVCDKCYD
                     RLFGSNLNKKMTNYEKIKNMSKIEMAEFLANGGSGCTSCAYDFQDCLGGCLEGRKKWL
                     ESEEGQI"
     gene            complement(4081..4239)
                     /locus_tag="KPG09_000581"
     CDS             complement(4081..4239)
                     /locus_tag="KPG09_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965209.1"
                     /translation="MKTKIIGGRENECLICNGNIFKIETLIGIVWQCKDCGCMYQDMS
                     SKESKRGY"
     gene            complement(4268..4852)
                     /locus_tag="KPG09_000582"
     CDS             complement(4268..4852)
                     /locus_tag="KPG09_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0965210.1"
                     /translation="MMKIFHFKLDGDYMRSLEEKNKLFEDNINLVHFIINRYFKSFLR
                     RYPYLKEDLFQEGYIGLYKTTCCFDESKGKFSTIAFSYISGHLKRFTTGYVKKHYRND
                     IDSFEKCIYRDNYGEEIRIEDRLAGNENVDIENVRVIRSCIKRSEIKDIQKIVCLREK
                     GYTQQEISKVLGTSQTSIYRRLKKLKTEINILGK"
     gene            complement(4830..5207)
                     /gene="ssb"
                     /locus_tag="KPG09_000583"
     CDS             complement(4830..5207)
                     /gene="ssb"
                     /locus_tag="KPG09_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG0965211.1"
                     /translation="MNNVVLVGRLARDPELRYIPEYGTPVATFALAVDRGYAKKDGTR
                     EVDFIPIEVMGGSAEFCANYLTKGRMVSIQGQIRIEKYEKDGEKKTFTKVRTKIVNAL
                     DHKPKDEEKEIGFQVLDDEDIPF"
     gene            complement(5204..5386)
                     /locus_tag="KPG09_000584"
     CDS             complement(5204..5386)
                     /locus_tag="KPG09_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965212.1"
                     /translation="MKSISHMNILERAEFTDKIANAIMSANKEIERGCAFNEAVEIVK
                     QMEYRENKYNEGDIAI"
     gene            complement(5444..6148)
                     /locus_tag="KPG09_000585"
     CDS             complement(5444..6148)
                     /locus_tag="KPG09_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965213.1"
                     /translation="MIRNVMEYLVKLGKRETVCVDGFKYTTDALEIIKEPKANELEIT
                     TLSGLVDYIKSGIDHEEDELLLIQVLTPRKVLLKSALRKNRDRETYIECVALLPDKRF
                     DRPLDLDTFNIMLQSAFIKNNDRDTLLKIAGNIQESTVKNVGDDGVSQSVTIKTGVAS
                     VSEAIVPNRVKLIPYRTFQEVEQPESEFIFRMSTGPAAGLYEADGGAWKNQAMLNIKA
                     YLQEELKDFKNVNIIA"
     gene            complement(6198..6584)
                     /locus_tag="KPG09_000586"
     CDS             complement(6198..6584)
                     /locus_tag="KPG09_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965214.1"
                     /translation="MIALEKFAGGVLKEKFNTELQKVLDNIADPNTDFKKTRKISLEI
                     VFKANEDRDLAEVDIKSKATIVEAKATTTKVIIGKDLETGRVEASEFKNQLAGQLSID
                     VSDSNETDEIEENSSGVIDFRNASSK"
     gene            complement(6959..8290)
                     /locus_tag="KPG09_000587"
     CDS             complement(6959..8290)
                     /locus_tag="KPG09_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0965215.1"
                     /translation="MNYSLEAEQNILGSFLINDSIGYKIRELKENDFYFESHKIILSC
                     MKKIIDSNKPLDLLLLKNELEKIDRLVDIGGVSYITSLTTIVITTSNIDHYIKIIKEK
                     LLKRQISELANELINNSNSDTSIDELIVDINDLKALVTTSSSINDNYIDASKIKREKG
                     VHKSIDTGFNKLNNMLDGFRYGTLTILTGKPASGKSTIVNQFIAEAIVNKEKAFLYSG
                     ELPAFMAMEWFRKTVANDYHIKEYKSVYGGTYTDIPDYASELISDWIEDKLFLYDEDA
                     ISDEVNLLNTIEHLYLKKGVRFFVLDNLMTIKTGNKADKYERQEQIVSNLKNLAKKYN
                     LVIVLVAHPRKNMGDMKPTMYDVSGASEIVNYADYILSTYRVVDEEEETDDTYLLILK
                     NRITGKQNISFKMNFSERRKRLYTTSEELNRDYKYDVNKQYVQVEIPEDVF"
     gene            complement(8312..8629)
                     /locus_tag="KPG09_000588"
     CDS             complement(8312..8629)
                     /locus_tag="KPG09_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965216.1"
                     /translation="MERKKHELTDQDIQRLIEGLRTDPKIREMIFESKDCETRTSTRS
                     KCEQFVEEKLAYRSYGFETPKYYAKLIADIIDILVYKNVPRDRICGVLYDVERVIPLV
                     VTF"
     gene            8761..8973
                     /locus_tag="KPG09_000589"
     CDS             8761..8973
                     /locus_tag="KPG09_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965217.1"
                     /translation="MNMCKIKTLREKCSLIDDKVEFSGEYKTFPGFTGSKLLECECNK
                     NYICKIQIMTANSNCPLFKKARQLQY"
     gene            complement(8992..9210)
                     /locus_tag="KPG09_000590"
     CDS             complement(8992..9210)
                     /locus_tag="KPG09_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965218.1"
                     /translation="MKEEKLELTEKDLHCIARHLQNEVMEMVFRGNIEAPTSCEVCDY
                     LQECKGYFTHIDTFIKLSEMTGVDIFTK"
     gene            complement(9236..9421)
                     /locus_tag="KPG09_000591"
     CDS             complement(9236..9421)
                     /locus_tag="KPG09_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965219.1"
                     /translation="MNEKEEAVYLNSSNVKLFLGVRDIKEFEELIENVNERICQLQES
                     INKLAQFNIEFDIKSDI"
     gene            complement(9450..10181)
                     /locus_tag="KPG09_000592"
     CDS             complement(9450..10181)
                     /locus_tag="KPG09_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0965220.1"
                     /translation="MDLLKDARKREWFWLENDLVDREDLGIYEKMIYIVLARYSDNES
                     CCFPSYKTIALKCGCSERQAKSVVKILENKGLIKKENRIKSNSNEKESNIYFVLTAKL
                     GGEYDAQQVVNMMHNPSAPHALPVVHEVHSKKTYNKKTYIKSNTTPQNEPKTDYLDLS
                     FLDLDIEKVKLTKDEYDKLISKFGKKYIHDKIVSLENYIVNGKGSRYKSHYRALLTWG
                     NADTSKGILQPVTKAKNPLSGFKEL"
     gene            complement(10211..10429)
                     /locus_tag="KPG09_000593"
     CDS             complement(10211..10429)
                     /locus_tag="KPG09_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965221.1"
                     /translation="MNDEMITKMFSLNKTYKMTSIRLNLEVKGVITHIDKNEIVLEYY
                     DNELECITTKILTIDDIKYNDYNLKPLH"
     gene            complement(10446..10622)
                     /locus_tag="KPG09_000594"
     CDS             complement(10446..10622)
                     /locus_tag="KPG09_000594"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965222.1"
                     /translation="MKKVDTKKISKSIKESTEFGILYSSLKDKQRDRVLGIMEGMKIA
                     NELSENNFMDIKHS"
     gene            10781..11152
                     /locus_tag="KPG09_000595"
     CDS             10781..11152
                     /locus_tag="KPG09_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0965223.1"
                     /translation="MENIKDESVLKTLREALGLNQSELANILKMTPSAISKIEKNGKP
                     LSDRISSQLSKEFGVREEWLKNKEGKMFADSSDDDLVAEIAANIINSDDKFMKNVIIA
                     FNKLTEDQRNFLINFMKELDK"
     gene            complement(11736..12251)
                     /locus_tag="KPG09_000596"
     CDS             complement(11736..12251)
                     /locus_tag="KPG09_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0965224.1"
                     /translation="MSKMIQCKSCSKEIASNAKSCPNCGAKNKKPFYKRGWFIVVVVL
                     VLLYAIGTSGSGTDDTAESNDDGTSKTASVDKPEEPKKEKYEIVGDITSETDSMATYI
                     KGVIKNNSGHDISYAQVTFTLYDKDGNQLGTAMDNINDLKKDGTWKFKAMGMNTDGEI
                     ASYELSEITGF"
     gene            12625..13008
                     /locus_tag="KPG09_000597"
     CDS             12625..13008
                     /locus_tag="KPG09_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0965225.1"
                     /translation="MKIKKIPESELLVMMYIWDSEDEEVASTEILKTLGEKYEWKKST
                     MLTFLRRLVGRGFLEVVKKDRFTYYKALVEKEDYLKVETKSFFSFFHKNSFESLLSAL
                     HDDEEVSENKLKSLEKFIEDWEEDK"
     gene            complement(13157..13846)
                     /locus_tag="KPG09_000598"
     CDS             complement(13157..13846)
                     /locus_tag="KPG09_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBG0965226.1"
                     /translation="MLENRKQIQKEIYLPIATYCLKDKQYYIKEYGALLIKSAENSDI
                     FNTHKNRILYKNKLDIQEIANELNVNKATIQRNIKKLEKLDFKILKIENTLNGIVYCL
                     PSENNIDLNKFALINYEMLKKMVNKFKSNTIKVYFLLKTITTETNFKPATNSFIAENI
                     GLSSKSKNNLDIITSSVKTLEENKYIETKKVNVYEYDKEKLREVPKIRKVYRICNFDE
                     WKKEIDKNKVY"
     gene            14266..15453
                     /locus_tag="KPG09_000599"
     CDS             14266..15453
                     /locus_tag="KPG09_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBG0965227.1"
                     /translation="MEKKEILMQPNNLIKSKYDFTNVENKLFYKILFNAQKQQNSSYV
                     TTISKEELKVFMKNNNDYEHKNIKEILNMFQQSVLEFDYIEETTGKLKTFGSGLINTY
                     ELDHTDQIYTIMMHEVLYNHITDFVKMQKKKNGYTAINLSVLFNFRGAYTQRLYTLFR
                     LWSRENKEVEIKYKLDELRFYLKLKDNVYPEYKYFKQNVLKRAMNEINKKGNMVVSIK
                     EEKRKNRKIDEIIFSVIDYEPRKYFDKDILVEDYIPKKKQDNEVSFSFYIPNENIFTT
                     GTIKLFKEDFKEFDFKKDVYLNAFNKSIAIAFERDNTDIINTKNYDFFKSTLSNKISE
                     VIIHQKDDLKFKEELDKYWDDEDVKKEEREVDYSKDPVRLETVRRNLFDQGLVNEEGY
                     YYK"
     gene            15473..16273
                     /locus_tag="KPG09_000600"
     CDS             15473..16273
                     /locus_tag="KPG09_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG0965228.1"
                     /translation="MYYVYKYIDPATEECLYVGKTEDIYARHSSHLSNKEEDWCTKEL
                     ILKYIELPRRYNMDFFEVYLINELNPIFNKLSKGDMDTEHIFFDYKEEDWSTYSEEDF
                     KEKTKGKRRFRERKIDMKYEVSKKSINFLKRIEKLDAKVEMLYENNVLSVLYNFENMD
                     IEFCENELDINLISYDFETLERFGSCSLISVYPRWKEYTATNKKILINITLEIRIGSL
                     ANLMELMPTFEYKITEIFQQIEDIFHILEVGYTWQELFEIYKGCKYDN"
     gene            16293..17027
                     /locus_tag="KPG09_000601"
     CDS             16293..17027
                     /locus_tag="KPG09_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG0965229.1"
                     /translation="MYYVYKYINQDTEECLYVGKTENICDRHASHLSNKKENWCNKNL
                     RLEYMELDNKYTMDFYEIYLINKLEPKFNITGKGEMDIAKTSFSYNGQWVIYLERDLM
                     SSLASKRYGINYEVNAKMLGIMRKLKKISSNEEIFIGNENIRIKYYFETVLEGINLEP
                     CILSVAYKTLKESTVGTVISVKREYLSENVCLIIDSIFLNEIMKDPLMSKSDIKDLNS
                     QVNDILKIIGVDCEWNKLSDYCSANS"
     gene            17147..17299
                     /locus_tag="KPG09_000602"
     CDS             17147..17299
                     /locus_tag="KPG09_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965230.1"
                     /translation="MDNFLFNVLASLTASVVVYLISELFKKAKSHSRAKSDLQVEFKF
                     IFKFKK"
     gene            17687..17989
                     /locus_tag="KPG09_000603"
     CDS             17687..17989
                     /locus_tag="KPG09_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965231.1"
                     /translation="MEVNLHTGKDEFFKNHEVAKNYIGGLYKLIDVYKFSMESVSVLT
                     RIDLDKLNNFYAGEASLNYDELCLIESVIITPLVGAFETAEHNYNMIIKARKERKL"
     gene            18024..18482
                     /locus_tag="KPG09_000604"
     CDS             18024..18482
                     /locus_tag="KPG09_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965232.1"
                     /translation="MKKTIALLLTLCAVMIPFTAFADGNDYEGTGNSQTVELIGQPNS
                     DSPSTKSIVVPGGTANLTAMSGGQLSWRVTSTLGNILGFEGEINVYKAGKIVSSQLVG
                     FRLAGTTKTGVVSFKGLKKGSYIAKFKGKGVCTGGIILFSSPAEASFTIR"
     gene            18712..18915
                     /locus_tag="KPG09_000605"
     CDS             18712..18915
                     /locus_tag="KPG09_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965233.1"
                     /translation="MSRIVLTFKNNDKEKAIEKFLDEKLSATAYLKELVWEKMNEKKD
                     NAVVEQKKEIEDPANNFDFGSLE"
     gene            18934..19839
                     /locus_tag="KPG09_000606"
     CDS             18934..19839
                     /locus_tag="KPG09_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HBG0965234.1"
                     /translation="MSKLGIDIGNYAVKTSTDDIFESKVTEVKNFGSDSDSIKIGNKT
                     YYLGEGDEEINIVKYEKENFLPLLLGAICRNTDDEVIDLALGLPVKQFGGLRKDLIEK
                     LQGKEYHVEFEKGNETTKRDITIRSVQVFPEGVTGYLYYAKDIVDQIAGRDVVLVDIG
                     GKTTDIALVQGNKATDPYSVNVGTINIYDAIKKSLEMDERFLGKVEIKREKIQDYIDK
                     GFYLNGEKQDIKKNIDASVGLFKQIYNELKLNYPISTSAVVVMGGGAKLLGEAFKKNI
                     PGIIVMSDVDKHVFANAKGYKKMMK"
     gene            19921..20436
                     /locus_tag="KPG09_000607"
     CDS             19921..20436
                     /locus_tag="KPG09_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965235.1"
                     /translation="MTWSDLKEKNINEVVDCINNKLSEFESLKKVGDELGANESTIRK
                     WLNKKGYKRVGNRFLEDNKSYDDKYQIDSINSKIDDDIKYLSNEIDNIKSVIEWFKTK
                     DDKCHIDSIKENDISIDLPDQSIKRTTIRVNNTVWEMFNKFADENKHYDKHDLLSQLL
                     LESLLKYVKSR"
     gene            complement(20447..20782)
                     /locus_tag="KPG09_000608"
     CDS             complement(20447..20782)
                     /locus_tag="KPG09_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0965236.1"
                     /translation="MLIQSLKRGELIVMKIFWKRNSRISRKEIIRICNEKHKWQKSTT
                     KILLKRLVKKRVLLKRIKIFHIYYIPIVTEKEYFKYKINDLEPDYLDGYFIRLMSTVH
                     KFKNLDEED"
     gene            complement(21116..21885)
                     /locus_tag="KPG09_000609"
                     /pseudo
     CDS             complement(21116..21885)
                     /locus_tag="KPG09_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894430.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(21944..22780)
                     /locus_tag="KPG09_000610"
     CDS             complement(21944..22780)
                     /locus_tag="KPG09_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0965237.1"
                     /translation="MKICITVGHSILKSGACTSADGVINEYQYNKSLAPVLADIFRKE
                     GHKVDVIICPEKQFKTKSEEKSYKIPKINSGNYNLLIELHLNSSGVGAFGTEVFYYSE
                     KGKEYAQRVVDKLSKPFIRKKGDKEVGNRGAKLDKSLYILNSSKPTAVLIESFFCDNK
                     EDYEKAKKLGHEGIAKLIVEGILNKNINNEGVKQMYKHTIVYDGEVDKIPATVVGWGY
                     NDGKILICDIKDYVPGQTQNLYVVGGGACEKIGSITKEHYTMIKGNDRFDTLYKALDF
                     IK"
     gene            complement(22780..23271)
                     /locus_tag="KPG09_000611"
     CDS             complement(22780..23271)
                     /locus_tag="KPG09_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG0965238.1"
                     /translation="MHLKDLDNSRSFLIQKLGGNMNLTIVFLATNIFIKLVILAIAFD
                     TLLGCLRAIKTHQFNSSFGINGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQDWIDF
                     LRLNHLGISEFFSILFILYESVSILKNMYLCGLPVPKKLKEKIGNLLDTMTDELNIKG
                     GNK"
     gene            complement(23279..23497)
                     /locus_tag="KPG09_000612"
     CDS             complement(23279..23497)
                     /locus_tag="KPG09_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965239.1"
                     /translation="MKKIIVICILVLMLIIPTMGEIFIMVENIKLEGLEQNPTSCYIS
                     NPGIMGEAKCDAAYFIIAADFVNNLKVK"
     gene            complement(23595..23777)
                     /locus_tag="KPG09_000613"
     CDS             complement(23595..23777)
                     /locus_tag="KPG09_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965240.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFELDDVTNTEYRKAVED
                     YIIRESGVVEGAEPTK"
     gene            complement(23777..24070)
                     /locus_tag="KPG09_000614"
     CDS             complement(23777..24070)
                     /locus_tag="KPG09_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965241.1"
                     /translation="MNVPNRVIYDQTGKIIFETGESCGDVLPHNKITELDYIDIPYGS
                     IDYIKNRIIGINIETKQPILEEIPVYVSEEKKRIQELENQVLLNENEKVGGLL"
     gene            complement(24086..25573)
                     /locus_tag="KPG09_000615"
     CDS             complement(24086..25573)
                     /locus_tag="KPG09_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965242.1"
                     /translation="MSTTVLERTVKRRRGYYRMTDIHASRLTYNDGSPYYTDFVAYYT
                     LDQYERVSISATKKFVAYSTRACQIINGREVDISRNFTQETTVQFVPDPTIFISNDLG
                     VIGNACSINYRISDSDSSVRFKIIEKINGVKIAEKNNVVDGNYELIITDELLSELAFN
                     SVNNITIELDNGYGGIFLDKTVTFTKGNTKPKLNITSYNSTSATFTAIDTDNNLSKIE
                     WFIDDVLKETITIDLTTEKTINYELADNAIHTLKIVATDSENATAEKVLSISKEIMPL
                     QSDASLSDISTKLIEIGEGFRNGKTSIINTLALKNIEASLNNTLVELSEKIKQSFDSG
                     DASLQDLMNQLTQANNTISQLNSKYKVASGTVTSFADSAKIAYPYLTDRTFKPGTWVK
                     ISNLDFKPNIFFADFDYYDTEYKNNYKLFLFACRGVATQRGVDFSSVTAFIRKNSDEN
                     FHANGWLYNNSEGDVYFNNTGVQIPAYNFDSTQRHIYKWYAIKFI"
     gene            complement(25593..26375)
                     /locus_tag="KPG09_000616"
     CDS             complement(25593..26375)
                     /locus_tag="KPG09_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG0965243.1"
                     /translation="MAQAQYYTLLTEIGKAAIANATALGTRVDFAKIKVGDGGGSAYI
                     PTETQTELKNVVWESTLEHVQADEKNPNWVVIQKTITGDTGSFTIREIGVFDSKDQLL
                     AVSSYPETYKPAPDSGTVKEILIKIILAVSNTASINLKIDPTVVLATLRDIQDLDAKI
                     DTTKTELTSNIETTKTELNTKIGDTTQLTTTDKTNIVSALNEVKSSVDSIETTAEKTS
                     IKDTDNLFSSDNVEGALKEVMQEVKGNRSSIISTVNNNLIPM"
     gene            complement(26376..26990)
                     /locus_tag="KPG09_000617"
     CDS             complement(26376..26990)
                     /locus_tag="KPG09_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBG0965244.1"
                     /translation="MKLIDYLPSFANNEIDIQIQEALENELLTLIDEKDDLLEQFFID
                     TATWGLDDWENLLSIKVNYKLDFDTRRSNIKAKMRGKGTTTIEVIKAISEAYTKTNVD
                     VEVFSNLFSFTLSFITNDCSYNTILELDKKIEEIKPAHLEHKFEMILFNENGLYAGAM
                     SSTGETVTIYPYTPKNIECFGEIILASGNNKSAERVTLYPQEVI"
     gene            complement(26992..28044)
                     /locus_tag="KPG09_000618"
     CDS             complement(26992..28044)
                     /locus_tag="KPG09_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG0965245.1"
                     /translation="MYSGQSFSTLNNRTLNNINLPLYKNQGSSLYNIVSPINSELAQL
                     YIELSYIHKRVFIQDNFDDFLDRRVNEFGVYRKLGTEAIGEVIFEGKIGTKIPNGTIV
                     SHSDLLFVVIKDVTIDEDSKLNVSLIQALEVGIKYNLSANTEFKLIEEINGVTKIYNE
                     LDLKGGTEIETDEELKERFYKIQKNQATSGNKAHYQSWALEVEGVYNAKVIPRWDGPG
                     TIKILIYGQNNQSVDNEVLQRCIEHIEEEKPIGPTITIVTPSTFDINISATLTLENGY
                     DIESIKVVFLDIINSYLIENSREIIYIKIMSLLASIEGVHDIKNLLVNDDVKNIIVDE
                     EKAPAVSSAIFDIEVS"
     gene            complement(28037..28471)
                     /locus_tag="KPG09_000619"
     CDS             complement(28037..28471)
                     /locus_tag="KPG09_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG0965246.1"
                     /translation="MNNTIFPFMGVPEDYISPDNEELPIFKEFAWDFDKDEKVIENGD
                     FKIVERNEAIKVWIYKTIKTDKYVYLIYDWDYGTDIKNLIGQKYTKGLTESEAKRYIQ
                     EALLTNPYILEVNVTNAEFKDDNLSISLNVKTIYGEEEITFV"
     gene            complement(28464..28796)
                     /locus_tag="KPG09_000620"
     CDS             complement(28464..28796)
                     /locus_tag="KPG09_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBG0965247.1"
                     /translation="MADPINEFIGIMRQEGKFHNEPSFFIGKIKSKLPDLKIEINNII
                     LEKEDILVDSWMLDRQIELFDTETSQEHKHEIKNPFIDTFESGNTVIMFKIGEKFAVV
                     SKLVSLDE"
     gene            complement(28810..30327)
                     /locus_tag="KPG09_000621"
     CDS             complement(28810..30327)
                     /locus_tag="KPG09_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0965248.1"
                     /translation="MIIDRSKDSSNNNIDFTAKDMGFLLLQSEVSYNFKDKLVEDIAK
                     QVFVDNKFPLGNLPKTNVKYTKMFIGVTGYDTIMSVYTEASKTTKKKYMIESNLDKFN
                     VIEKGVVTLNITFEEGFNLINTNFSESMENVKNKVLVVDQYGNKISEKINDSIFKDVG
                     VIMQKVIQQQENSTIDIDGEFKGIEKTCSLKGYGDITCVTGRGVKVKDSYTKLIGLFY
                     IDTDKHTWQNGDYQIELELNFENIMDEKSAGQDEQKEDGSEYTGGTEYSAEFTAYCPR
                     KSEGGDKDCRGKKLDPSKKTCAAPMVGTYEKSYYTKDFLNKHPLMRYGDEIQLVTGVS
                     SRDGTYKVNDNGPAIIIEKDGTYHIDILFGNVEEANNFGRRKGKIIIGGYSGNVTEKA
                     KTVISEARKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVNLPRTSSQQSKVGKKVEK
                     NNLQAGDLVFFHNPVSHVGLYVGNGEFLHAPQTGDVVKISKLSNRKDFNTARRVL"
     gene            complement(30567..31202)
                     /locus_tag="KPG09_000622"
     CDS             complement(30567..31202)
                     /locus_tag="KPG09_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG0965249.1"
                     /translation="MEIWLRQANNTFRFPVIPPSFEINGSATINTSNILSVGDIAVFG
                     GLGLKTIELSSFFPNQEYSFCNYNGFPKPYDCVNLIESWMKEGYILRFIITETNINFE
                     CIIADFNYREQDYSRDVYFTLSLKEYRRIQISKVNINNDEKLSSEKDVPLTKGFDTKQ
                     KTHKVVEGDTLFKIAKKYYGNGDLWEKIYKANEDKIKDPSVIKNGWILIIP"
     gene            complement(31260..31736)
                     /locus_tag="KPG09_000623"
     CDS             complement(31260..31736)
                     /locus_tag="KPG09_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBG0965250.1"
                     /translation="MNKKIPALAILIVLSLSLVGCSTNDSKNGDSKPKQNTEEIKNHK
                     IGEVVDLDGAKVSVKNVRKLESDSNVYAVEIEITNTGDTDLESDFLQNYILKTDDNTV
                     GNAINNQELNGELLFDTIHPGDTLKGEIAFELTKDTKPKTIEIAYENVGKYTVFDL"
     gene            complement(31783..35172)
                     /locus_tag="KPG09_000624"
     CDS             complement(31783..35172)
                     /locus_tag="KPG09_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBG0965251.1"
                     /translation="MSSNAKVLQAIIKMRDEASSTLKRVAANTRALHDSSSQASRALN
                     NVQSNLKSVGTSALKAGGLITAGVGGAFAAATKVGTEFESAMSQVKATFGNSLKPGDF
                     EALSKAARDAGKATTKTAADSANALQYMGLAGWDVQKSMHALMPVLNLSEAASMDLGL
                     ASDLVTDSMSSMCLEVDKVSKSGKVLAGAGLSEYLDKVANASTKSNTSIQQLMEAFNV
                     AGGTFSNLKVPLSEATAIMGILANRGVKGTDAGHALNSIMVNLTTGAGQAGKAMKKLG
                     VSAFDNQGKFKGMSNVLKEVANKTKGMTDEQKNYYLSAIGGKTQLKTLQKLLDGVGNE
                     YDSLKKKIDNSKGALKTMSDVMKDNTKGGLATLKSALEETGITIYYQLKPAIASVVDN
                     LTKMTVWFNNLSEPIKRVITNVMLSVGAFGVLLLTIGAVSLIVSKAIGVFGFFVPIIL
                     RAKVAFRAFVIGMKPLSAVFLAVMGPVGWTITAVIALAVAFTIAYAKSEVFRNKVNSL
                     VKSFTEFAKPIVNFCMPALKQLGEKFKELLSAFGPLFASILNLGSVIMKVLGPVLIGV
                     IGFHIAKLVLILSTLATAFKAVVDTITNVLNGLASILNGVFNLIIGIVTGDSKKMMNG
                     VKQIFDGGVKIIKSIWKGFIDFVTAPIQAIADIADSLFKDKAKWLTDKWKDLRTFLEN
                     PIEATAKISTKTNVNIPGSKAADAAGKSTPNTGGLKQTIANLKGSFSDFKTFIKPMTD
                     SISQGVESGKTKFTELQNKILNDVKPALKVLEDSGVNFKDKFKESITSIKDKFNELKN
                     ILAGNIKTNIDNLVKAFEPLKPHLDNLKECFDKVKKAITDFFTPAKQVGDTVNKVSQP
                     MDSVKKSTDGIKLSFQSLTQALAPVKAWFQGFMPFLSQIGQQLLPSIGIAIAGVVGGI
                     ILAFTWVLNTLTAIITTVSGIINGIMSFISGIINVIMGLITGNADQTMDGLKQIFDSG
                     VGVIKSIWNGLVDLVSGVLEPVIDLADSFFKDKVKGVKEAWNKLKDFFKHPIKGTIEI
                     FKKGPSLQIGKGADAAGKSPRKAFGINRVPRNDYLIRAHEGEKLLTKQEANQYKRNSS
                     NGSLFLDKLADTIIVREEADIDKIVRKINKKISLAKVGGVL"
     gene            complement(35176..35325)
                     /locus_tag="KPG09_000625"
     CDS             complement(35176..35325)
                     /locus_tag="KPG09_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965252.1"
                     /translation="MYLLFKYKGIMPSVSYRILRGEKEIVSVFMDQELDERKEEIKSG
                     AGRLF"
     gene            complement(35352..35768)
                     /locus_tag="KPG09_000626"
     CDS             complement(35352..35768)
                     /locus_tag="KPG09_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0965253.1"
                     /translation="MNTVEKLLNMDAGKLKMPSSIFELYCKKLDDILEIECNAIDPER
                     FDEIRMNSMDVDSGNLENINVYELKVNTILESCPMFRNMEVVENLGLATPKDLLKKLL
                     LAGEIDNLYEEVNKVNGIDAEGDKKRQKERAKDIKN"
     gene            complement(35784..36224)
                     /locus_tag="KPG09_000627"
     CDS             complement(35784..36224)
                     /locus_tag="KPG09_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG0965254.1"
                     /translation="MKAKIDPSRIISGTFGECWIDGRQMMAVKGLEAKVELEKEEVKV
                     CGQLMVGQKLIGASGKGSVNLFKVSSLFGEYVESMLNEGKAFKFTIISKLQDPDSYGA
                     ERVALYGCMLDDLTLADWEAGKMGEIEVPFTFESFKYLDKITEK"
     gene            complement(36240..37295)
                     /locus_tag="KPG09_000628"
     CDS             complement(36240..37295)
                     /locus_tag="KPG09_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG0965255.1"
                     /translation="MGLPEINISFIQAGKTFVKRSGGIVALILKDTKNIGLVEIKEME
                     DIPENYSATNLDYIKMAMKGNVYPPNKVLVYTLDTDESIDDALDFLETCEFNYLCMPD
                     EEEGDLPKIKTWIKKMREDNKIKVKAITASNSADYEGIINFTATDIEVEGKKYTSNEF
                     LPRIAGFIAGTPSTQSVTYAEIPEVTNIPKLTRAEANTRINNGELILIKESGAIVIAR
                     GVTSFTTITDSKGDIFKKIKLVDTLDQIHNDIKKIIVKNYIGKTQNTYDNKCLLIVAI
                     QLYLQELEKDGLIDPGSTIEINLDAQRAWLKENHIDVSNMEEQQIKEYNTDTIVFLKA
                     KIKLIDAMEDVYLDISM"
     gene            complement(37296..37745)
                     /locus_tag="KPG09_000629"
     CDS             complement(37296..37745)
                     /locus_tag="KPG09_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965256.1"
                     /translation="MLTNRDILIAVNEILKNEFIDIDIFINEDNIQGFEEACFFVQLL
                     PVSNNIANKILNTKKIFIDIEYYQKARKNKLNLYDIQSRLEKIFNRNIRVKDRSLTIE
                     NISPSIVKDSVGHKLTFLMTISYFEEVYFSKETYVTMENVDLKIGSD"
     gene            complement(37754..38110)
                     /locus_tag="KPG09_000630"
     CDS             complement(37754..38110)
                     /locus_tag="KPG09_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBG0965257.1"
                     /translation="MGIDNLGVFEALALDLGLNASNFNRKIKKFTNKTGQELAENIRD
                     ETPVRTEKLKNGWEESQPEYDTYIVEDKVEYATLVNDGHATKNGGIIPGSFMVEKGIE
                     KTKENMTSAKANLFDW"
     gene            complement(38110..38457)
                     /locus_tag="KPG09_000631"
     CDS             complement(38110..38457)
                     /locus_tag="KPG09_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965258.1"
                     /translation="MTEADILEMTYLDKMTIIRKSKGWNQETCSNDFSNIIIAENIPC
                     AISRKEEAIISGDIGAIVINRKLFCRPEVDIKTGDTINATYSHGEEAVFKVSKANIYP
                     SHMEIPITEAERI"
     gene            complement(38469..38849)
                     /locus_tag="KPG09_000632"
     CDS             complement(38469..38849)
                     /locus_tag="KPG09_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HBG0965259.1"
                     /translation="MSTMLDNVKLVLGIENDRYDKLIILYINKISEMVLEYCSRQELS
                     IALEGFVEEKVISILSSRFEELKKNSGLDNLEKSLKAITRGDTKIEYNALTLQTAENS
                     ISSSSFLTDTDKKFLSPFCIVNMY"
     gene            complement(38833..39105)
                     /locus_tag="KPG09_000633"
     CDS             complement(38833..39105)
                     /locus_tag="KPG09_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965260.1"
                     /translation="MFALIKNNIIKVVEDENSRDKLISKGFKIYSEEVTKENIEKKEE
                     KVKSTSNDLFNLSKPELMEIAKGKNIKGYSTKTKEELIQLLDEYNA"
     gene            complement(39119..40042)
                     /locus_tag="KPG09_000634"
     CDS             complement(39119..40042)
                     /locus_tag="KPG09_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965261.1"
                     /translation="MANVLQYVTLFQQELDMQVTVGSTTGWMEKNAGQVIYNGGREVK
                     VPKVTMDGLGDYDRSKGYTQGAVTLEYETMKMEMDRGRTFMLDSMDVNESNFVINASS
                     VMGLFQKQHVIPEIDAYRYSKIFSETAKVDERVKYSYEATVTTILSELKNQLASIQDL
                     SGDIPLIISMSSIIASILEQSSQLDDIEFGDGDIKRIVKSIDNVPIIKVPSKRMKTEY
                     VFRDGKTSGQTEGGFTEGAKALDINWLITPASAPIAVSKTDKPRIFTPDQNQTADAYQ
                     IDYRKYHDLWILDEYLKLCRASVRQAKAAQE"
     gene            complement(40071..40673)
                     /locus_tag="KPG09_000635"
     CDS             complement(40071..40673)
                     /locus_tag="KPG09_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBG0965262.1"
                     /translation="MLKKEVLELLKEIEDDAEIDSLLQSTDLFKSASDKKLTIEEFRE
                     LINNDANFKAIIDNEKNKYHSEALENFKKKDMQTLISAEVLKRTGANETEEQKAIREL
                     RESLDKLKKEKQYAEKVSKYKDILVEKKIPTNLIEYLLSDDDDKTNANIEIFENSMKQ
                     YVQSKVDERIKDGSYVPPGKDSAGSLSEIRKQIKQGLNSL"
     gene            complement(40699..41025)
                     /locus_tag="KPG09_000636"
     CDS             complement(40699..41025)
                     /locus_tag="KPG09_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965263.1"
                     /translation="MKVEILGTEYLVIEKLESEDVLLKERAGYCDHSVKEIVIEKINS
                     EEGSLKDLSVYKNEVARHEIIHAFLSESGLKSCSSWATNEEMIDFFAIQFPKIVKVMD
                     KIECLN"
     gene            complement(41084..41845)
                     /locus_tag="KPG09_000637"
     CDS             complement(41084..41845)
                     /locus_tag="KPG09_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBG0965264.1"
                     /translation="MNQKIITKLTEDIYKSAENRTKPFYKYQRENRDKLLLEIAKILL
                     THDIINEYLNINNKDKKNLRSKLNKIINEYSNSYDEEVKDIETILSDSSKNKYSNLII
                     LLGVILKIKDVSNINNKVINKIVNNKVDGKHWSDRLWKNKKGIEESLKKEIKSFLNGK
                     TSVNKIEKAIRDKYSTGAGQTSRLIENEVARCQSEVNEYFFKVQGIIKVMYCADLDSR
                     TCSDCSMHNGVIFYVNEDRPSLPRHCHCRCQYIPV"
     gene            complement(41823..43100)
                     /locus_tag="KPG09_000638"
     CDS             complement(41823..43100)
                     /locus_tag="KPG09_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0965265.1"
                     /translation="MELDLDLIKDIYDDWNSNKSEYDTMYKYYKGETDAISNYKMVTK
                     RSNNKINTNFLKKFINEEVAYSLANKITYTSRSGDEKIINDLEYYTCHWSKKHDSDLL
                     RYALLFGLCYELYYVKDDEIQVKIIKPTDGCHYENENGEIVCFIREFTKGFEDDTYVD
                     VYDKEYIYHFDSNFKEVESPTVNNIFDGNVPISICKRSEELGKNTIFNDIKGLQDAYE
                     TNLSDISNEISDFRNAYLTFSGCNIKEDDLPRMKELGILQVNGDGKIEWLIKDMNDTF
                     VQNTLSSIKENMYEITSHINHNEKMQSNTSSLAVIARLISTEWICSQNNDSIADTLFN
                     RYKLLCIWLNKKYGFDYDYKDIKAKFTPKIPRDDLVVANILSQLGDKLSTETGLSQLS
                     FIDNPHAEIEKAKKEQEIVSEGEILLDESKDYN"
     gene            complement(43113..43316)
                     /locus_tag="KPG09_000639"
     CDS             complement(43113..43316)
                     /locus_tag="KPG09_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965266.1"
                     /translation="MGGNLLANRSKFEEVQKTLTKKGKKFNVGIGKDEKGYFAYTHRA
                     RSKSYISKQDIPIKVLKFIESTG"
     gene            complement(43317..45056)
                     /locus_tag="KPG09_000640"
     CDS             complement(43317..45056)
                     /locus_tag="KPG09_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965267.1"
                     /translation="MDNLERNIQLIFSYLAKTFIRNGASIKDAEKEANEIIKANSNNL
                     WGVNGLAYQLGKINLEFFCMYFMQDTYLPKEDNAAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGRKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKRFSDDVKYAAQKPVIRNGKTIKRGTTFIALGTLQHKECF
                     YSRLKNLPTWKFKCEKGVLVDNVDELFNSGLWLKFKEILFDFKNTNHLEDAKEFYWEN
                     EKEMKFPILWPSFWDCLDMALSYYENPTSFKQEVQNDVNSIGEKWFKTVRTETREEIE
                     SHTFKKTMLLCDPASAGGSKHDYSAFLVGSESENGLLYGRLAELAKINARTDFDKYID
                     HMIYLLKVYSDITHIYIEKNTFNGADANQLEFKIKNDNVLSYRDIEIINEQQKKNKDD
                     KISTLIPVLSKGQMIFAEEDKEFIQQILDFTGQKYSLHDDAPDISSEFINRINNIKVS
                     ETVNIFDRKLLGF"
BASE COUNT        14528 a         7376 c         5310 g        18045 t
ORIGIN      
        1 aaaaaaaaat aaatatatta aagtattttg aattattttc ttgcaagaaa tatttttttg
       61 attagaatta ttaattatct ttcaactatt ccttaaatat gtattttcgg catagttaaa
      121 aagaacctag catttccaat aggttcacat atcctataat aatccacatc acttcactgt
      181 atgaaacttt aattattaag tctcactaaa ttttattaaa ttagctataa ctaaagttaa
      241 atacaatata tgatttaatt attaaagtaa atatatatat aatatatgtt ttgctttaat
      301 tgatttcaaa cataattaaa acatattctt gcaggattta tttcatatct aattacttct
      361 tgcttgttct tattcttatc ttagctatag ctgcttctat ctctgttgca tccataccca
      421 tgccaacact attatcctct gcttgtataa ctgtacttgt aggagttcca tatatacgat
      481 ttataagata ctgattggct gccaacataa ccctcttatc gctcttgtca ctagctaatt
      541 ccttgatatt gttgatataa ctatctaaat cctttaatat cattaaattt ccttggtttg
      601 ttagctcctg tttccgtctg tccatttctt tcctaacatg ctctttattc atccaattat
      661 agattgtttt tctatttact ccaataattc tggcagtttc tgttactgta gcacctgtaa
      721 tcaatagagt taccatattg cactgatctt ctgtcaactc tttacctttt tcatagctac
      781 tatcgattat ttcaacttca ttcacatcca attaatcacc tctttactca gcttctaaat
      841 aaacatttaa atatctacat ccctataaat cccttgaata aatgaccttt aatatatatt
      901 ttcagacatt ttaataaatc tatcctattc tttggaaatt ctattattga ggctctttac
      961 attctaataa tgtcctgttg ttgaaaaacg taacatatta aattttagat aaaatacaag
     1021 atattttaat ttctttttct tttctttaac ttattagtaa atttagttat tgcttttact
     1081 tcttcatcct tatctattcc tatatacctt ctagtcattt ctattgtctt atgtcctaga
     1141 atctttctta taagttctaa tgcataagtc ttattttctt cttcatcata aatatgcctt
     1201 gcaaatgtct ttcttaaaga gtgtgttgca acattttctt ttattcctac cttttctgct
     1261 gctcctttta ttattcttcc tgcttgcctt gtttggatag gcctattaaa tttcttcctc
     1321 ttgccacttt taaataaaaa ctcttctcct ggtttatctt gagtatagtt ttctatagca
     1381 ctctttatta catcagataa gaaaacttct ctatcatagt ctcttttttc ttctataact
     1441 ctaatccagt ccttagacac tcttatcttt tttccttttt tatctaactt tatatctgtt
     1501 acatctgcaa ctttaagtgc taatatatct cctattctaa gtccagtaca cattccaaag
     1561 agggctaata agtaatttct ttcgttccat tcctctagaa aattcaagag tctaaataca
     1621 tcctctatat cttttattgg attaactttt tgtccactat gtgcttccat tcattaaaaa
     1681 cactccttat taccatgata attttttctt tgcctatctt gcaccaatat ttttaagtct
     1741 tcaactacat tagcaaaagg atctgtgatt atagaggtta tatcatttgt actatatttt
     1801 ctttctcttg cataagtatt ggtctttatt ctaaatccta agtcatttac agtttcagtt
     1861 acttttacaa cattttctaa ctgcaaatat aatttaaaga caaccaactc aacgtgatca
     1921 gactcatcaa ttatactttc ctctaaaatt ttcagatatg agtctatctt attaatatta
     1981 gaatcataat ttttcttaat cttctttttt agtttcaaga gtttttcttg ctttttcttt
     2041 ataaaatcta aagaacattc tcttccatac atgctatgag tttctctcat cacactgtca
     2101 taatacttta aattatctgg tacatatgca gcacttgaaa tttcttctgt tatatcacgt
     2161 ttaatcttca acacctctct agacaataaa aaagacttag aacgtaaatt ccaagccatt
     2221 ttttattaaa tatatttttc tcacaataca atcttatcat gattaaaaga cacaaaacag
     2281 acataaattg gtcacaaatc ggacattttt tagacacaaa acagacataa atcggtttaa
     2341 aatcgtcaca aatcggacat ttttatagca atccttttat cttatctata atatcaaatc
     2401 tcatttgctt acacttactc gctgaaaaac caatcattct gccaacatat tcccaagatg
     2461 tcctgctact tgaaaagtat ttatactgta ctatcttttt ttcttcttca cttaatattt
     2521 tcattgcatt atctattttt tctattatca actctttatg tctcttctct ttttctttta
     2581 aggcaatttt cttttctttt tcttcagtct ctatttcaat tacactttta atatcatatg
     2641 tcttgcttgt cttttcttta atttcaactc cactacatcc tctgtagtcg ttttttatca
     2701 tctgcatatc aatgtctatt ttttctattt ccgcttttat ttttttataa ttatataact
     2761 taccttctgc actactaaac aaattgtctt ttttgctaga ttgcattacc tcacactcct
     2821 aattatgtta taatgatgtt gtgatgacgg ttttagaatt ttgacaagca ggagcgtgaa
     2881 gtaatgctcc ttttttcttt tttgaaacat atcttattct ttaggcacta attgcaactt
     2941 gtaatcatca gaactatata atttataacc tcttaccttc aagtctaatt ctagttgact
     3001 taaactttta taagcaccaa atgaaccata tccattctgc atatttataa taacataccc
     3061 acctattgtt tcctttaaag aaagaatcat atataactta ttaccaccat taatttcaac
     3121 aatatctcct acttttaatt ctctattatt ttcagttact tctatcaaca tattatatta
     3181 tctccttact ttaattaata ttttttagaa actctcccat tttcatttca tcaatgtttt
     3241 cattaattcc tttcatagca cttaagttaa ccaaaggcac gcacaaatcc catagcttat
     3301 ttacatctaa aaattgtatt tttgaattaa attctttttc atcaacgcat tttaatttat
     3361 tattcatatt aattaattca ttaatcattg aatttttaat taaattaagt ataccatctc
     3421 ctatttcttt gagatcccat cctcttccat ttaatttctt attatgtatc tctatacctt
     3481 ctgcaatata atcttctaat tctaaaattt gaaacattgt taaaatagtt tggcaaacat
     3541 ctaatccttc tgcaattata tctttggtca ttcctgtatc tatagcctct ttaaactcag
     3601 ttacttcttc atcaattttt gccaactgat cctctacctt ccaattaggt atactgctaa
     3661 ttaatttcaa atccatcttt aatatctccc ttcattcatt tattgaagtc ccaacctttt
     3721 ttgagtatgc ttattcatga atattatttc atatctggcc ttcttcactt tcaagccact
     3781 tttttcgacc ctctaaacat cctcctaagc aatcttgaaa atcataagca caactagtac
     3841 atccacttcc gccatttgca agaaattcag ccatttctat tttgctcata ttttttatct
     3901 tttcataatt agtcatcttc ttattcaagt tacttccaaa taatctgtca taacatttgt
     3961 cacatacatc ttttccgaca ctcattatat taccttcatc atcaattctg aataccttta
     4021 tgtttagaat ttctgctatt gtttcaccac aaaaatcaca ctttataaca caactcatct
     4081 ttaatacccc cttttacttt ctttagaact catatcttga tacatacatc cacaatcctt
     4141 acattgccac actattccaa ttaatgtttc aatcttaaat atattaccat tacaaattag
     4201 acactcattt tccctaccac caattatttt agttttcatt ttacctccaa taaacttaaa
     4261 tcatttatta tttacctagt atattaattt cagtttttaa ttttttaagt ctcctatata
     4321 ttgacgtttg gctagttcca agcactttac ttatttcttg ttgagtataa cctttttctc
     4381 ttagacaaac tattttctgt atatctttta tctcacttct ttttatacaa gatctaataa
     4441 cacgaacatt ttctatatct acattttcat tgccagccaa tctatcttct attcttatct
     4501 cttctccata attatctcta taaatacact tttcaaaact atctatatca tttctataat
     4561 gtttcttaac atatccagtc gtaaatcttt tcaaatgtcc tgatatatac gaaaatgcta
     4621 ttgtagaaaa ttttccttta ctttcatcaa agcaacatgt tgttttatat aatcctatat
     4681 atccttcttg aaataaatct tcttttaaat atggatacct tcttaaaaag gatttgaaat
     4741 atctatttat aataaaatgt acaagattta tattatcttc aaataattta tttttctctt
     4801 ctaaacttct catataatca ccatccaatt taaaatggaa tatcttcatc atctaaaact
     4861 tgaaagccta tttctttttc ttcatccttt ggcttgtgat caagcgcatt aacaattttt
     4921 gttcttactt ttgtgaaagt ttttttctct ccatcttttt catatttttc tatccttatt
     4981 tggccttgaa ttgaaaccat tcttcctttt gtgagataat tagcacaaaa ttctgctgat
     5041 cctcccataa cttcaattgg tataaaatca acttctctag ttccatcctt ttttgcataa
     5101 cccctatcaa ctgccaatgc aaaagtagca actggagtac catactctgg tatatatctt
     5161 aattctggat ctcttgctaa tcttccaact aaaactacat tattcatatt gcaatatctc
     5221 cttcattgta tttattttct ctatattcca tttgttttac aatctcaact gcttcattaa
     5281 aggcacaacc tctttcaatt tctttatttg cagacataat tgcattagca attttgtctg
     5341 taaattctgc tctttctaaa atgttcatat gagaaatact tttcatttaa ctccctcctt
     5401 gttttactaa gaggacttat aataagtcct cttagatttt caattaagca attatattta
     5461 catttttaaa atcttttaat tcttcttgca agtacgcttt aatatttaac attgcttgat
     5521 ttttccatgc tccaccatct gcttcatata atcctgctgc tggtccagta ctcattctga
     5581 aaataaattc actttctggt tgctctactt cttggaaagt acgataagga attaatttta
     5641 ctctgtttgg aactattgct tctgatacac tagcgacacc tgtttttatt gttacagatt
     5701 gagaaacccc atcatctcca acatttttta ctgtagattc ttggatattt cctgcaattt
     5761 ttaaaagtgt atctctatca ttatttttta taaatgctga ttgtagcatt atattaaaag
     5821 tgtctaaatc taaaggtctg tcaaatcttt tatctggaag tagtgctaca cattcaatat
     5881 aagtttctct atctctattt tttctcaaag cagattttaa aagcactttt cttggagtta
     5941 agacttgtat taataacaac tcatcttctt catgatctat gccacttttt atataatcta
     6001 ctaaaccact caatgtagtt atttctaatt cattagcttt tggttctttt attatttcta
     6061 atgcatctgt tgtgtactta aacccatcta cacatactgt ttctcttttt cctaacttaa
     6121 ctaaatattc cattacattt cttatcattt tttattctcc tttttattta aatttatatt
     6181 ttatttactt tcttaatcta ctttgaactt gcatttctaa aatcaattac accagaacta
     6241 ttttcttcta tttcatcagt ttcattacta tctgaaacat ctatagacaa ctgaccagct
     6301 agttgatttt taaattcaga tgcttcaact cttcctgtct ctaaatcttt tcctattata
     6361 actttagttg ttgtcgcttt tgcttctact atagttgctt tggattttat atctacttca
     6421 gcaagatctc tatcttcatt tgctttaaac actatctcta aactaatttt tcttgttttt
     6481 ttaaaatctg tatttggatc tgcaatgtta tctaaaactt tttgtaactc tgtattaaac
     6541 ttttctttta gaactccacc tgcaaatttt tctaatgcta tcatgtaaaa tcactccttt
     6601 atttatttaa aataatattt ttctacgagg aaattaatta ataaacttta gctctaattt
     6661 aataacactt ttaaaacttt ttataaattt atatagcctt tttatcctta cactactttt
     6721 aagttttttt gctagcatat ctaaacctgt gtgtacattg tcaggaaatt aatttatata
     6781 aaccttgtaa atgctatatt ttcaaacttt ttttagtatt gaaattcaat actttttcaa
     6841 aatccgtttt tcaatattga aaaatgattt ttttcaatat tgaattttta aattattcaa
     6901 tattggtttt tcttataaaa tcattactta ataaaatttc ctatttttta tacttttttt
     6961 aaaatacatc ttctggaatt tctacttgaa catattgttt atttacatca tatttgtaat
     7021 ctctgtttaa ctcctcacta gtcgtgtaaa gtctttttct tctttctgaa aaattcattt
     7081 taaaacttat attctgtttt cctgttattc tattttttaa aattaataaa taagtgtcgt
     7141 ctgtctcttc ttcttcatca acaactctgt aagttgataa aatataatct gcataattaa
     7201 caatctcact tgctccagac acatcataca tagttggctt catgtcaccc atatttttac
     7261 gaggatgtgc aactaaaact attactaagt tatatttttt agcgagattt tttaagttgc
     7321 ttactatctg ctcttgtctc tcatatttat ctgccttgtt gccagttttt atcgtcatta
     7381 agttatcgag tacaaagaac ctaacccctt tttttaaata taaatgttct atggtattta
     7441 gtagatttac ttcatcactt attgcatctt catcatagag aaaaagttta tcttctatcc
     7501 agtctgaaat aagttcagag gcataatctg gaatatctgt gtatgttcct ccatagacgc
     7561 ttttatattc ttttatatgg taatcattag ctacagtttt tctaaaccat tccatagcca
     7621 tgaaggcagg caattctcca gaatacaaaa atgctttttc tttatttaca attgcttctg
     7681 ctataaactg gttaactata gttgatttac cagatgcagg tttaccagtc aaaattgtaa
     7741 gagttccata tctaaatcca tctagcatat tgtttagttt attaaaacct gtatctatgg
     7801 acttgtgtac gcctttttca cgctttattt ttgacgcatc aatataatta tcatttatac
     7861 tactgctcgt cgtcacaagt gcttttaaat cgtttatatc tacaatcaat tcgtctatac
     7921 ttgtgtctga atttgaatta ttaattaatt catttgctaa ttctgaaatt tgtcttttaa
     7981 gtaacttctc ttttatgatt tttatatagt gatctatgtt tgaagtagtt ataactatag
     8041 ttgttaaact ggtaatgtaa ctaacgcctc caatatctac taatctatct attttttcta
     8101 attcattttt taaaagcaaa agatctagtg gtttatttga gtcaattatt ttcttcatgc
     8161 aagataaaat aattttatgt gactcaaagt agaaatcatt ttcttttaat tcccttattt
     8221 tgtaacctat agagtcattt ataagaaaac ttcctagaat gttttgctct gcttctaaag
     8281 agtaattcat ttaatcaccc ccagcttatt attaaaatgt cacaactaaa ggaatcaccc
     8341 tttcgacgtc atacaatacc ccacaaatac gatctcttgg aacatttttg taaactaaaa
     8401 tatcaattat gtcggcaata agttttgcat agtattttgg agtttcaaat ccataacttc
     8461 tataagcaag tttttcttca acaaattgtt cgcatttact tcttgtactt gttctagttt
     8521 cacaatcctt actttcaaat atcatttctc ttatttttgg atcagttctt aatccttcaa
     8581 taagtctttg tatatcttga tctgtaagtt catgtttttt tctttccact tagcatacct
     8641 cctgtcttta actatttagt tgcttctttt gatttgaaac tattctacac catgataatt
     8701 tgcatgttat aattaattgg agcatattga cagtatgttc aaattaatta tagggtggtg
     8761 atgaatatgt gcaaaattaa aaccttaaga gaaaaatgtt ctcttattga cgataaagtt
     8821 gaattttctg gtgaatataa aacttttcct ggattcactg gatcaaaact tttagaatgc
     8881 gaatgtaata aaaattacat ttgcaaaatt caaataatga ctgctaatag caactgtcct
     8941 ctttttaaaa aagcacgtca attacaatac taaaatttta atttactaaa tttatttagt
     9001 aaaaatatct acacctgtca tttcacttaa ttttataaaa gtgtctatat gggtgaaata
     9061 gcccttgcat tcttgcaagt aatcgcaaac ttcgcatgat gtaggggctt ctatattccc
     9121 tctaaacacc atctccataa cttcattttg taaatgtctt gcaatacagt gtaaatcttt
     9181 ttcagtaagt tctaactttt cctctttcat gagcaacctc ctaatttata cttaactaaa
     9241 tatctgactt aatatcgaac tcaatattaa attgggcaag cttattaatc gattcttgga
     9301 gttgacaaat tcgctcattg acattttcta tcaattcctc aaattcctta atatccctaa
     9361 cacctaagaa taacttcaca ttagaactat tcaaataaac agcctcttct ttttcattca
     9421 tcctatctac ctcctatact atttagtttt taaagttctt taaaaccact gagtggattc
     9481 ttagcctttg taactggttg caatatacct ttgctagtat cagcattccc ccaagtcaac
     9541 aaagctctgt aatgactctt atatctactc cctttaccat ttacgatata gttttctaaa
     9601 ctaacaattt tatcatgtat gtatttcttc ccaaacttgc ttataagttt gtcatattca
     9661 tctttagtta gttttacttt ttctatatct aaatctaaaa atgataggtc taaataatca
     9721 gttttaggtt cattttgtgg agtggtatta ctttttatat aagtcttttt attataagtc
     9781 tttttactgt gcacctcatg cacaacaggt agtgcatgag gtgcactagg gttgtgcatc
     9841 atattcacta cctgttgtgc atcatattca ccacctaatt tagctgttaa aacaaaatat
     9901 atgttgcttt ctttttcatt tgagttactt tttattctat tttctttttt aattaaccct
     9961 ttattttcta aaattttaac tacgcttttg gcttgccttt cactacatcc gcatttcaaa
    10021 gcgatcgttt tatagcttgg aaagcaacaa ctttcattat cagaatatct tgcaagaact
    10081 atataaatca ttttttcata gattcctaaa tcttctctat ctactagatc attctctagc
    10141 caaaaccatt ccctttttct agcatctttc aagagatcca tttcatcacc tcctttctaa
    10201 aagatatttc ttagtgtaaa ggttttagat tataatcatt atattttata tcatctattg
    10261 tcaatatttt agttgtaatg cattctaatt cattgtcata gtactccaat actatttcgt
    10321 ttttatctat atgagtaata acgcctttta cttctaaatt taatcttata gaggtcattt
    10381 tatatgtttt atttaagcta aacatttttg taatcatttc atcattcact ttaatacctc
    10441 ccaacttatg aatgttttat atccataaaa ttattttctg acaattcatt tgctatcttc
    10501 ataccttcca taattccaag aactcgatct ctctgcttgt cttttaatga agaatacaat
    10561 attccaaatt ctgttgattc ttttatgctc tttgatattt tctttgtatc tacctttttc
    10621 attattattc acctctcttg taatatgatt ttattataaa tcattcatgt gaaagtttca
    10681 ataaaaatat gaaaatttca caattatttt ttattttttc atttttgttt tgattttttc
    10741 acatttatat tgtataatta tacttaagag gtgattaaaa ttggaaaata taaaggatga
    10801 aagtgtttta aaaacattaa gagaggcact ggggttaaat caaagcgaat tagcaaatat
    10861 tttaaaaatg acaccttctg ctatatctaa aatagaaaaa aatggcaagc ctttaagtga
    10921 tagaatttcg tctcaattga gcaaagaatt tggcgttaga gaagaatggc ttaagaacaa
    10981 agaaggcaag atgtttgcag attcatcaga tgatgattta gttgcagaaa tagcagcaaa
    11041 tataattaat tcagatgaca agttcatgaa aaatgtcatt atagctttta acaagcttac
    11101 agaagatcag agaaactttt taattaattt tatgaaagag ttagataaat aaagccgtta
    11161 aaataacggc tttatttatt gagaattgtt tttataaata ttaagattga ttttaatttt
    11221 tttacatcat ttgtattttc aattgaattt ataatttcct tttttaaatc tcccatagaa
    11281 tcccccatat ttttaaaata ttctgattta tatttttaca attacattgc ttctgaaaaa
    11341 taacagactt ccccctttta caatttttaa atttttatca gctactaaat taatgttttt
    11401 tgcttttgta tgtcatgcct tagatgagtt tgaaaatttg attgtattca atattgaaaa
    11461 ttaagatttt tgattatttt taaaaatatt ttacgtacgt agttttatat gcattttata
    11521 tgtaatttcg atatatatct tctttatgtt acaattgtaa cacctcattt aacattaatc
    11581 aaatgtaaaa ttttaccaat ttaaattatt ttttaaaatt taattttaca ttaatagaat
    11641 ttatttatat aataaattaa aaaaggacta tggactatag aattttactt ctatagtcca
    11701 tagtcttcct ataatttagg attgatataa atcttttaga aaccagttat ttctgataac
    11761 tcataagatg caatttctcc atctgtattc attcccattg ctttgaactt ccaagtacca
    11821 tcttttttaa gatcgtttat attatccatt gcagtaccta gttgatttcc atccttatca
    11881 tataaagtaa atgttacttg tgcatatgat atatcatgac cactgttgtt tttaataaca
    11941 ccttttatat acgttgccat actatcagtt tctgatgtta tatctccaac tatttcatat
    12001 ttttcctttt taggctcttc tggtttatct acactagctg ttttacttgt tccatcatca
    12061 ttactttcag cagtatcatc agtaccactt ccactagtac caatagcgta taataaaact
    12121 aaaactacta caactataaa ccaacctctt ttataaaatg gttttttgtt tttagcccca
    12181 cagttaggac atgatttagc atttgatgct atttcctttg aacatgattt acattgaatc
    12241 atttttgaca ttttaaaatc cccctctaat taatttatga tgacctcaat catattatag
    12301 tatacaacta atgtctaaaa ctgtaatatc atgacatcaa aagaatttta ttaggtatat
    12361 agcaaatatt ttgttgtact aaatggcatt caataaagac tttcacttat attgtataca
    12421 ttttttcgaa ctgcttttaa gttataatat taaataaata atttatttaa tattataata
    12481 cgtctttata attagcatat taaagtattt tttataatca gaattaattg gaaaaaattg
    12541 gaatgcataa ttagacattt ttcattgtaa attaaattct attgtaatag tattatagta
    12601 tatcaaaaaa cgggggtgta gtacttgaaa ataaaaaaga ttccagagtc agaacttttg
    12661 gtaatgatgt acatttggga tagtgaagat gaagaagttg catctactga aattttgaaa
    12721 acattaggcg aaaaatatga atggaaaaaa tcaactatgc taactttttt aaggagatta
    12781 gttggtagag gatttttaga ggttgttaag aaagatagat ttacatacta caaagcattg
    12841 gtagaaaaag aagattattt gaaagttgag actaaaagtt tctttagttt ctttcataaa
    12901 aattcatttg aaagtttatt aagtgctttg catgatgatg aagaagtatc tgaaaataaa
    12961 ttaaaaagtt tagaaaagtt tatcgaggat tgggaagaag ataaatagag agccaatata
    13021 attttatttg gttctctatc tatatatttg accttctatt tccattttta agcaaatttg
    13081 tatcttttta ggtaaataac atcaagtttg taattaaaac acgtcagaca agcctttaaa
    13141 tgcttttaag ctatatttaa taaactttat ttttatcaat ttcctttttc cactcatcaa
    13201 aattacaaat ccgataaact tttcttattt ttggaacttc tctaagtttt tctttgtcat
    13261 attcatatac atttactttt tttgtttcaa tgtatttatt ttcttctaaa gttttaacag
    13321 aagaagttat gatatctaaa ttatttttac ttttagaact tagtcctata ttttctgcta
    13381 taaaactatt agttgcaggt ttaaaattag tttctgtagt gattgtttta agtaaaaaat
    13441 atacttttat tgtatttgat ttaaatttat tgaccatttt tttaagcatt tcataattta
    13501 tcaatgcaaa tttgtttaaa tcaatattat tttctgacgg taaacaatat acaattccat
    13561 ttagggtatt ttctattttt agaattttaa aatctaactt ctctagtttt tttatatttc
    13621 tttggattgt tgctttattt acattcagtt catttgctat ttcttgaata tcaagtttat
    13681 tcttatacaa aattctattt ttatgggtat taaaaatgtc actattttca gcactcttta
    13741 ttaatagtgc tccatattct tttatataat attgcttatc ttttaaacaa tatgttgcta
    13801 ttggtaagta aatttctttc tgaatttgtt ttctgttttc aagcataaaa aattcttcat
    13861 ctccaatctt ttataagaaa ttatttttag taagtgttta aagtatacta gaaaaataaa
    13921 tactaaagtt ttttaatata aaaaaagaat agttcagaac atcttttcta gtctttaata
    13981 gtttttaaaa cagtttttaa agtctttaat agtttttaga caagctataa taattggtat
    14041 atctaggttt cagagatgaa aggtcatcat tttaagggtc aaaggtcatc attttaaggg
    14101 tcaaaggtca tcattttaag ggtcgaaagg tcatcatttt aagggtcaaa ggtcatcatt
    14161 ttaagggtca aaggtcatca ttttaagggt caaaggtgag cgtctttcaa aaatagttga
    14221 cctttttata aattaggtat tataatttat atgaggtgat agattttgga aaaaaaagaa
    14281 attttaatgc aaccaaataa tttaataaaa agtaaatatg attttacaaa tgtggaaaat
    14341 aaattatttt acaaaatact ttttaatgct cagaaacagc aaaattcatc ttatgttaca
    14401 actatatcaa aagaagaact aaaagtattt atgaaaaaca ataatgatta tgagcataaa
    14461 aatataaaag aaattttgaa catgtttcaa caaagtgttt tggaatttga ctatatagaa
    14521 gagacaactg gcaagttaaa aacttttgga agtggtttaa ttaatacata tgaattggat
    14581 catacagacc aaatttatac aattatgatg catgaagttc tatacaatca tataactgat
    14641 tttgtaaaaa tgcagaaaaa gaaaaatgga tatacagcta ttaatttatc agtgttattt
    14701 aattttagag gtgcatatac tcagaggtta tacactttat ttagactatg gagcagagaa
    14761 aataaggaag tggagataaa atataagtta gatgaattaa gattttattt gaaattaaaa
    14821 gataatgtat atccagagta taaatatttt aaacaaaatg ttctaaaaag agcaatgaat
    14881 gaaataaata aaaaaggcaa catggttgtt tcaataaaag aagaaaaaag aaaaaataga
    14941 aaaatagatg aaataatttt ttctgtgata gattatgaac ctagaaaata ttttgataaa
    15001 gatatattgg ttgaagatta tatacctaaa aagaagcagg acaatgaagt gtcttttagt
    15061 ttttatatac caaatgaaaa tatttttaca acagggacga ttaaactatt taaagaagat
    15121 tttaaagagt ttgattttaa gaaagatgtt tatctgaatg cttttaataa gtctattgct
    15181 atagcatttg aaagagataa tactgatatt ataaatacaa agaattatga tttttttaaa
    15241 tcaacacttt caaataaaat aagtgaagtt ataattcatc aaaaagatga tttaaagttt
    15301 aaagaagaat tagataaata ttgggatgat gaagatgtta aaaaagaaga aagagaagtt
    15361 gattatagca aagatcctgt cagacttgaa actgttagga gaaatttatt tgatcaaggt
    15421 ctagtaaatg aagaaggata ttactataaa taaaaattgg agaaaataaa aaatgtatta
    15481 tgtttataaa tatattgatc cagctacaga agaatgttta tacgttggaa aaacagaaga
    15541 tatatatgca aggcattcat ctcatttgag taataaagaa gaagattggt gtactaaaga
    15601 attaatatta aaatatattg aacttccacg tagatataat atggattttt ttgaagttta
    15661 tttaataaat gaactaaatc caatatttaa taaactaagt aaaggtgata tggatactga
    15721 acacatcttt tttgattaca aggaagaaga ttggagtaca tattcagaag aagattttaa
    15781 ggaaaagaca aagggaaaaa gaagattcag agaaagaaag atagatatga aatatgaagt
    15841 aagtaaaaaa tctattaatt ttttaaagag aattgaaaag ttagatgcca aggttgaaat
    15901 gttatatgaa aataatgttt taagtgtgct atataacttt gaaaatatgg atatagaatt
    15961 ttgtgagaat gaattagata ttaatttgat atcatatgat tttgaaacat tagaaagatt
    16021 tggaagttgt tctttgattt cagtttatcc cagatggaaa gaatatacag caacaaataa
    16081 aaaaatactt ataaatataa cattagagat taggattgga tctttggcta atttaatgga
    16141 attaatgcca acatttgaat ataaaattac tgagattttc caacaaatag aggatatttt
    16201 tcacatacta gaagttggat atacatggca agaattattt gaaatatata agggctgtaa
    16261 atatgacaat taagaattgg agaaaataaa aaatgtatta tgtttataaa tatattaatc
    16321 aagatacgga agaatgttta tacgttggga aaacagagaa tatctgtgat agacacgcta
    16381 gtcatttaag taataaaaaa gaaaattggt gtaataagaa tttaagactt gagtatatgg
    16441 aattagataa taaatatact atggattttt atgagattta cttaataaat aaactagagc
    16501 ctaaatttaa tattactgga aaaggagaaa tggatatcgc taaaacatca ttttcctata
    16561 atggtcagtg ggtaatttat ttagaaagag acttaatgtc gagtttggca tcaaagagat
    16621 atggaataaa ttatgaagtg aatgctaaga tgttaggcat aatgagaaaa ttaaagaaga
    16681 taagcagcaa tgaagaaata tttattggaa atgaaaatat aagaataaaa tattactttg
    16741 aaacagtact agaaggaatt aatttagaac catgcatatt gagtgtagca tataagacac
    16801 taaaagaaag tacagttgga acagtaatct cagtaaaacg tgaatattta tcagaaaatg
    16861 tatgtttaat cattgatagt atttttctaa atgaaataat gaaagatcct ttaatgtcta
    16921 aatcagatat taaagattta aatagccaag tcaatgatat tcttaaaata ataggggttg
    16981 attgtgagtg gaacaagctt tctgattatt gtagtgcaaa ctcttaactg atattacaaa
    17041 atatggtata attaaattta gcaagaagag cgtaatatac aatctataga gtggagttca
    17101 tgtacaaaag attatcctcc caacgattcg aggggaggtg agttacatgg ataacttttt
    17161 gtttaatgtt ttagctagtt taacagctag tgtggtagtt tacttaatca gtgaactatt
    17221 caaaaaagca aaaagccact ctcgtgcaaa gagtgactta caggttgaat ttaaatttat
    17281 atttaaattc aaaaaataaa ctacgtttat tatgaactcc actctacgcc taaatagatt
    17341 gtagttcttc ttgcttttat tatatcacaa aatctttaaa atgatacaat tttatatcat
    17401 ttcttattat taagaaatag aaaaacactt taattgatta aagaataatt ttatataatc
    17461 ttgtaaaaat ataaaaagaa cacttaatat attagcttga aaaatattaa gtgttctttt
    17521 tttctattct atttaaataa aatagaatag aaaaaatgat tgaaaatatt attctatcgt
    17581 gatagaatga aaaaaacaga atattctacc gtgatagaat attctgtttt gaagaagaag
    17641 taaatattat ttgaaggtta aaaatataag aacaatggag ggaattatgg aagtaaacct
    17701 acatacagga aaagatgaat tttttaagaa ccatgaagta gcaaaaaact atataggtgg
    17761 attatataaa cttattgatg tttacaaatt ctcaatggaa tcagtttccg ttttaactag
    17821 aatagattta gataagttaa ataatttcta tgctggagaa gctagtttaa actatgatga
    17881 attgtgctta atagaaagtg taattataac tcctttggta ggagcatttg aaactgctga
    17941 gcataattac aatatgatta ttaaggcacg taaagaaagg aaactgtaat tgagaataat
    18001 ataaaaaaat agggggaaaa caaatgaaaa aaacaattgc tttattacta acgctttgcg
    18061 cagtaatgat tccatttaca gcttttgctg atggaaatga ttatgaaggt actggaaact
    18121 cacaaacagt tgaattaatt ggacaaccaa actcagattc gccttcaact aaatctattg
    18181 tcgttccagg tggtactgct aatttaacag caatgagtgg agggcagtta tcttggagag
    18241 taacatctac gcttggaaat attttaggat ttgaaggaga aataaatgta tataaggctg
    18301 gcaaaattgt atcatctcaa cttgtaggct ttagattagc tggaactact aaaactggtg
    18361 tagtaagttt caaagggcta aaaaagggtt catatattgc taaatttaaa ggaaaaggtg
    18421 tgtgtacagg aggtattata ttattctcta gtccagctga agcttctttt acaattagat
    18481 aattataaaa agtagaaaag agtgtttaga aatatatgaa acactctttt ttttatgcaa
    18541 aattataaaa agtgtgtttt taattatata caaaatgata aattttataa aaaattataa
    18601 aatattataa aaaatatctt ataaatctat tgaattacta tgtgaaattt ggtaaaatat
    18661 atgtaaatat taataaattt tataatattt tacaaaaaag gaagtggttg aatgagtagg
    18721 atagttctaa catttaaaaa caatgataaa gaaaaagcaa ttgaaaagtt tttagatgag
    18781 aaattatcag ctacagctta tttaaaagaa ctcgtttggg aaaaaatgaa tgaaaaaaag
    18841 gataatgctg tagttgaaca gaaaaaagaa atagaagatc cagctaacaa ttttgatttt
    18901 ggaagtttag aataaaaggg ggaaataaca taaatgagta aattaggtat agatattgga
    18961 aattatgcag ttaaaacaag tacagatgat atttttgaaa gtaaagttac agaggtaaaa
    19021 aactttggtt cagattcaga tagcattaaa ataggtaata aaacatatta tttaggcgaa
    19081 ggtgacgagg aaataaatat agttaaatat gagaaagaaa acttcttacc gcttttactt
    19141 ggggctatat gcagaaatac agatgatgaa gttattgatc tagcattagg tctaccagtg
    19201 aagcaatttg gaggattaag aaaagatttg attgaaaaat tacaaggaaa agaatatcat
    19261 gttgaatttg aaaaaggaaa tgaaactact aaaagagata taacaataag atctgttcaa
    19321 gtgtttccag agggagttac aggatactta tattatgcaa aagacatagt tgaccaaatt
    19381 gcaggaagag atgttgtttt agttgatatt ggtggaaaga caacagatat tgcgcttgta
    19441 caagggaata aagcgactga tccatattct gtaaatgttg gaacgataaa tatatatgat
    19501 gcaataaaaa aatctcttga aatggatgaa agatttctgg gtaaagttga gataaaaaga
    19561 gaaaaaatac aagactacat agacaaaggg ttttacctaa atggtgaaaa acaagatata
    19621 aagaaaaata tagatgcgtc tgttggatta tttaaacaaa tatataatga attgaaatta
    19681 aattacccaa tatcaacttc tgctgttgtc gttatgggtg gaggtgctaa attgttaggt
    19741 gaagcattta aaaagaatat acctggcata atagtcatga gtgatgtaga taaacatgtt
    19801 tttgcaaatg caaaaggata caaaaaaatg atgaaataaa aaaggattta gtttatagta
    19861 tgtataatag attacaaata tatgatattt gtaatctatt attttttgag gaggttttat
    19921 atgacttgga gtgatttgaa agaaaaaaat attaatgaag ttgttgattg tattaataat
    19981 aaattaagtg aatttgaaag tcttaaaaaa gttggagatg aattaggggc aaatgaaagt
    20041 actataagga agtggcttaa taagaaagga tataaaagag ttggaaacag gtttttagaa
    20101 gataataaaa gttatgatga caaatatcag atagatagca ttaacagtaa gattgatgat
    20161 gatatcaaat atttatctaa tgaaattgat aatataaaaa gtgtgataga atggtttaaa
    20221 actaaagatg ataaatgtca tatagatagc attaaagaaa atgatatatc tatagatctt
    20281 ccagatcagt ctataaaaag aactacaatt agggtaaata atacagtgtg ggaaatgttt
    20341 aataaatttg cagatgagaa taaacattat gataaacatg atttgctaag tcaattattg
    20401 cttgaaagtc tattaaaata tgtgaagagc agatgaagtg agatatttaa tcttcttcat
    20461 ctagattttt aaatttatgc acagtggaca ttaatcgtat aaaatatcca tctaaataat
    20521 ctggctctaa atcatttatt ttatatttga aatattcttt ttctgtaact ataggtatgt
    20581 aatatatgtg gaaaatttta attctcttaa gtagaactct ttttttaaca agtcttttaa
    20641 gtagaatttt tgtcgtagat ttctgccact tgtgtttttc attgcaaatg cgaataattt
    20701 ctttccttga tattcttgaa tttcttttcc aaaagatttt cattacaatt aattcgccac
    20761 gttttaagct ttgtattaac atgatgcact ccttgtaaat gtttataata tagtagcatt
    20821 tttttattta agatagtaca ggtaagttat tccagaaaaa aagcaacgct agaatgtttc
    20881 aaaactaaat atgataaata tcatataaat gtcattaaaa aatgatttat ttttattgtg
    20941 ataagttaaa taaaaagaat tagaagataa taaaattttc tagtcttttt atttatttaa
    21001 tcttagattt aacagatgcg taattgcaac gtataagatt attaaaagaa atagaaaaat
    21061 aaagaaagca aaagatgata gtttttttat ctattatcct ttgctttctc taatattagg
    21121 attttactat gtttttgtca attgcattgt ttctgtatgc tctttcaatt ctatttttac
    21181 cttgaagaaa ataatcgcta tctaattcta ttccaacaaa ctttctattt atatttgcac
    21241 aagctattcc tacactgcca gaaccaaaac aattatccaa aacaagttca ttttcgtttg
    21301 tataagtttt tataagatat tctaaaagat ctactggctt ttgagttgga tggatgcaat
    21361 ttgtttcttt attaaaatac aatgtattat taggataatt tgtatattct tgaacatatt
    21421 cttttgataa agtatgatgt ctatatattc catctttatt tttatttttc tttttacatg
    21481 taataggttt atctaatttt tctaagtttt gaggattgta taaaggagct tttttataga
    21541 atacattaat atcttctact tttcgaagtg gctggtattt agaaaatgcg aatcctgtag
    21601 atttatttta atccagtacc aactgtattt atagttttta atatttgaat ttatcaaact
    21661 agtagtaaat ggttgtgctg aaaatagtac tatagctcca ccatctttta taattctatt
    21721 atattgattc cagattgatt tgaaaggtat tattgtgtcc catttacaat tagttgtgcc
    21781 ataaggaaga tcgcatagaa ttaaatctat tgatttatct tctattagat ccattatttc
    21841 taaacagttt ccattgtata gtttatattt ttctatgtta agcatgttgc aagtcctcct
    21901 aagttgaata aattttatat tgattggata ttacaacaag ttattatttg ataaaatcta
    21961 atgctttgta aagtgtatca aatctatcat taccttttat cattgtgtaa tgctctttag
    22021 taatagaacc tatcttctca catgctccac cgcctacaac ataaagattt tgcgtctgac
    22081 ctggtacgta atcttttata tcacatatca gtattttccc atcattataa ccccaaccaa
    22141 ctacagttgc agggattttg tcaacttctc catcataaac tattgtatgt ttgtacatct
    22201 gtttaactcc ctcattattt atatttttat ttaatatacc ttctacaatt aacttagcaa
    22261 tcccttcatg acctagtttc ttagctttct cataatcttc tttattatca caaaagaaac
    22321 tttcaattaa tactgctgta ggctttgaac tatttaagat atataaactt ttatctaatt
    22381 tagcacctct attacctact tctttatctc ctttttttct tatgaaaggt ttagacagtt
    22441 tatctactac cctctgcgca tattccttcc ctttttcact atagtaaaat acttctgttc
    22501 caaaagctcc tacaccactt gaatttaaat gcaactctat aagtaagtta tagtttccac
    22561 tattaatctt aggtatttta tatgactttt cttctgattt agttttaaat tgcttttcgg
    22621 gacatattat tacatctacc ttatgccctt cttttctaaa tatatctgct aatactggtg
    22681 caagagattt gttgtattga tactcgttaa ttactccatc agcagaagtg catgctccac
    22741 tttttaaaat actgtgtcct actgtaatac atattttcat tatttattac ctcccttaat
    22801 atttaattcg tctgtcattg tatctagtaa attgcctatt ttctccttta attttttggg
    22861 aactggtaat ccacataagt acatattttt taatatactt acactttcat acaaaatgaa
    22921 tagaatagag aaaaattcag atattccaag atgatttaat ctcaagaaat caatccaatc
    22981 ttgtggcaac ataaataaaa agttaaactt tgtaagtata tcaactactg ctagaaaaaa
    23041 tatacatgct atcattgcaa cttttcttat tcctccattt attccaaaac ttgaattaaa
    23101 ctgatgtgtt tttattgctc ttaagcaacc taacagtgta tcaaatgcta ttgctaatat
    23161 tactaatttt ataaatatat ttgttgctaa aaaaactatt gttaaattca tatttcctcc
    23221 taatttttgt attaaaaagg acctagaatt atctaagtcc tttaaatgta ttaatttatt
    23281 attttacctt taaattgtta acgaaatctg cagctataat aaagtatgct gcatcacatt
    23341 ttgcttctcc cataatccct ggattagaaa tgtaacaact tgttgggttt tgttctagcc
    23401 cctctagctt tatattttct accattataa atatctcccc catagtaggt ataattaaca
    23461 ttagaactag aatgcaaatt acaattattt tcttcatttt ttaatctcct tttaatagaa
    23521 tttttttatt atagataaaa ataaaaaata tataactaaa aaaggaaatt atttttaatt
    23581 tatataaaaa aaacctattt tgttggttct gctccttcta ctactccact ttctctaatt
    23641 atataatcct ctactgcttt tctatactct gtgttagtta cgtcatctaa ttcaaattct
    23701 cgatttttta gagggtttaa tcctccgttt aatatcctct ctgctaatat tcttactaca
    23761 acattattta tattcattat aataatcctc ctactttttc attttcattt aataaaactt
    23821 gattttctaa ttcttgtatt ctcttttttt cttcacttac atatactggt atttcttcca
    23881 aaattggttg ttttgtttct atatttatgc ctataattct atttttaata taatctatac
    23941 ttccatatgg aatatcaata taatctaatt cagttatttt attgtgtggt aacacatccc
    24001 cacaggactc acctgtttca aaaattattt tgcctgtttg gtcatatatt actctgtttg
    24061 gaacattcat atttttttct ccttcttaaa taaatttaat agcataccat ttataaatat
    24121 gtctttgagt tgagtcaaag ttataagctg gaatttgaac accagtatta ttaaaataaa
    24181 catccccttc ggaattatta tagagccaac cattagcatg aaaattttca tcactgtttt
    24241 ttctaataaa agctgtaacg cttgaaaaat caacacctct ttgtgtagcg acaccacgac
    24301 aagcaaaaag gaaaagttta taattatttt tatattcagt atcatagtaa tcaaaatcag
    24361 caaagaaaat gttaggctta aaatctaaat tactaatttt aacccaagta ccaggtttaa
    24421 aagttctgtc ggttagatat ggataagcaa ttttagcact atccgcaaaa gaagtgactg
    24481 ttccactagc aactttatat ttagagttta attgcgatat agtattatta gcttgtgtta
    24541 actgattcat caaatcctgt aaactagcgt ctccactatc aaaagactgc tttattttct
    24601 ctgataactc aacaagtgtg ttattcaaac ttgcttctat attctttaat gctaaagtat
    24661 ttataatact tgttttacca tttctaaacc cttctccaat ctctatcaac ttagttgata
    24721 tatcacttaa actagcatca gattgaagtg gcattatctc tttgcttata cttagcactt
    24781 tttctgctgt tgcattttct gaatctgtag caactatttt taatgtgtgt attgcattgt
    24841 ctgcaagttc atagtttatt gttttctctg ttgttaaatc tattgttatt gtttccttta
    24901 atacatcatc tataaaccac tctattttag ataaattatt atctgtatct attgctgtaa
    24961 atgttgcaga agtggagtta taagaagtta tatttaattt tggtttagta ttaccttttg
    25021 taaatgtaac agttttatct aagaatattc caccataacc attatcgagt tcaatagtta
    25081 tattatttac agagttaaat gccaactcag ataagagttc atctgtaata attaattcat
    25141 agtttccatc aacaacatta tttttttcag ctattttaac accatttatt ttttcgatta
    25201 ttttaaatct aacactagaa tcactatctg atattctata atttatacta cacgcattac
    25261 caatcacacc taaatcgtta cttataaaaa tagtagggtc gggtacaaac tgaacagttg
    25321 tttcttgagt aaaattccta ctaatatcaa cttctctgcc atttattatc tgacaagccc
    25381 tagttgaata agctacaaat tttttagttg cagatataga aactctttca tattggtcta
    25441 aagtataata agcaacaaag tcagtataat aaggacttcc gtcgttataa gtaagacgtg
    25501 aagcatgaat atctgtcata cgataataac cacgtcttct tttgacagtt ctttctaaaa
    25561 cagttgtaga catactaatt tcaccttcct ttctacattg gtatcaaatt attatttact
    25621 gttgatataa tactacttct attacctttt acttcttgca tcacttcttt taatgctccc
    25681 tctacattat cacttgaaaa taagttatct gtatctttta tacttgtttt ctctgctgtt
    25741 gtttctatgc tgtctacact gctttttacc tcatttaatg cactaactat atttgtttta
    25801 tctgttgtag taagttgtgt tgtatcccct attttagtgt ttaactctgt tttagtagtt
    25861 tctatgttgc ttgttaattc tgttttagtt gtatcaattt tagcgtctaa atcttgtata
    25921 tctcttaatg ttgctagcac aacagttggg tctattttta aatttatact tgctgtatta
    25981 gacacagcta atataatttt aattaatatt tcttttactg ttcccgaatc tggagcaggt
    26041 ttataagttt cgggatagct agatactgcg agaagttggt ctttagagtc gaatactcca
    26101 atctccctaa ttgtaaagct tccagtatct ccagttatag ttttttggat aactacccaa
    26161 ttaggatttt tttcgtctgc ttgaacatgc tctaatgtgc tttcccacac tacatttttg
    26221 agttctgttt gagtttctgt tggaatatat gcacttccac caccatcccc aacttttatt
    26281 tttgcaaaat ccactctagt tccaagtgct gtagcgtttg ctatagccgc cttgcctatt
    26341 tctgtaagta atgtataata ttgtgcttgt gccaattata tcacctcctg tgggtataat
    26401 gttacccttt cagcactttt attgtttcca ctagctagta ttatttctcc aaaacattct
    26461 atatttttag gtgtataagg gtatatagta actgtttctc ctgtactgct cattgctcct
    26521 gcataaagtc cattttcatt aaataaaatc atttcaaatt tatgttcaag gtgtgcaggt
    26581 tttatttctt ctattttctt atctaactct aaaatagtgt tataactaca atcatttgtt
    26641 ataaaactaa gtgtaaaact aaatagatta ctaaatactt ctacatcaac attggtcttt
    26701 gtgtaagctt ccgagatagc ttttataacc tctattgttg ttgtaccctt acctctcatc
    26761 tttgctttta tattgcttct tctagtatcg aagtctaact tataatttac ttttatgctt
    26821 aaaaggttct cccaatcatc aagtccccaa gttgctgtat ctataaaaaa ctgttctaat
    26881 aaatcatctt tttcatcaat tagcgttaaa agttcatttt caagtgcttc ttggatttgt
    26941 atgtcaattt cattgtttgc aaaacttggc aggtaatcaa ttaacttcaa tttaactcac
    27001 ctcgatatca aatatagcac ttgaaacagc aggagctttt tcttcatcaa ctattatgtt
    27061 tttaacatca tcatttacaa ggagattttt tatatcatga acaccttcta tgcttgctaa
    27121 caaactcatt atttttatat aaataatctc tcttgaattt tctattaaat aagaatttat
    27181 aatatctaaa aacacaactt ttatagattc aatatcatat ccattttcaa gtgttaaagt
    27241 tgcactaata ttaatatcaa aagtacttgg tgtaacaatt gtaatagtag gtccaatagg
    27301 tttttcttct tctatatgtt caatacatct ttgcaatact tcattatcaa ctgattgatt
    27361 attttgacca taaattagaa tcttaattgt acctggtcca tcccaacgag gaattacttt
    27421 tgcgttataa acaccttcaa cctctaaagc ccaagactgg taatgtgctt tatttccact
    27481 tgtcgcttga tttttttgta ttttatagaa cctttctttt aattcttcat cagtttctat
    27541 ttctgttcca ccttttaggt caagttcatt ataaatttta gttactccat ttatttcttc
    27601 tataagttta aattctgtgt tagctgataa attatactta atgccaacct ctaaagcctg
    27661 tataaggctt acatttaact tactatcttc atctattgta acatctttaa taactacaaa
    27721 tagtaaatca ctatgagata ctattgttcc atttggtatt ttagttccta ttttgccctc
    27781 aaatattacc tcacctattg cttctgtgcc taactttcta tatacaccaa attcattgac
    27841 tcttctatca agaaaatcgt caaagttatc ttgaataaaa actctcttat gtatataaga
    27901 aagttctata tataattgtg caagttctga atttattgga gaaactatat tataaagaga
    27961 agatccttgg tttttataaa gagggagatt tatgttattt aaagttctat tatttaaggt
    28021 actaaatgat tggccactat acaaaagtaa tctcctcctc tccataaatt gtttttacat
    28081 ttaaacttat agataaatta tcatctttaa attctgcatt tgttacattg acttctaata
    28141 tatatggatt agttaataga gcttcttgta tatacctttt tgcctcactt tcagtaagac
    28201 ctttagtata cttttgccct attagatttt ttatatctgt tccataatcc caatcatata
    28261 ttaaataaac atacttatct gtttttattg ttttgtaaat ccatacttta attgcttcat
    28321 ttctttcaac tattttaaag tctccatttt caattacctt ttcatcttta tcaaaatccc
    28381 aagcaaattc tttaaaaata ggtaattctt cattgtctgg agaaatataa tcttctggaa
    28441 cacccatgaa agggaatata gtattattca tctagactca ccaatttact tacaacagca
    28501 aatttttcac ctattttaaa cattattact gtgtttccag attcaaaagt atctataaag
    28561 ggatttttta tttcatgttt atgttcttga cttgtttctg tatcaaataa ctctatctgt
    28621 ctatcaagca tccaactatc taccaagata tcttcttttt ctaatatgat gttatttatc
    28681 tctattttta aatctggtaa tttactttta atttttccaa taaaaaaaga aggttcatta
    28741 tgaaatttac cttcttgtct cattattcct ataaattcat tgattggatc tgccacgata
    28801 tcacctcttt tacaaaactc ttctagctgt attaaaatct tttctattac ttaatttact
    28861 tattttgact acatctcctg tttgtggcgc atgtaaaaat tctccattac ctacatatag
    28921 tccaacatgg ctgactggat tatggaaaaa taccaaatct cctgcctgta aattattttt
    28981 ctctactttc ttacctacct tagattgctg acttgaggtt cttggtaaat taacattaac
    29041 cttcttaaag caatatacca ttagtccaga acaatcaaag ctacttggtc cattcccacc
    29101 ccatttgtat ggctttccaa gatgttttct tgcttctgaa attactgttt ttgctttttc
    29161 tgtcacgttt ccagaataac caccaattat aatcttccct tttcttcttc caaagttatt
    29221 ggcttcttca acatttccaa ataaaatatc tatatgatat gttccatcct tttctataat
    29281 tattgcaggc ccattatcat ttaccttata cgttccatct cgacttgaaa cacctgtaac
    29341 caattgtatt tcatctccat atctcataag aggatgtttg tttaagaaat cttttgtata
    29401 gtaagatttt tcatatgttc caaccatagg agcagcacat gtttttttag aaggatctag
    29461 ttttttacct ctacaatctt tatctccacc ctcagatttt ctaggacaat aagcagtaaa
    29521 ttcagcagag tattctgttc ctccagtata ctctgaacca tcttcttttt gttcatcttg
    29581 accagcagat ttttcatcca ttatattctc aaaattaagc tctagttcaa tttgataatc
    29641 tccattttgc catgtatgct tatctgtatc tatataaaaa agacctatta attttgtata
    29701 tgagtctttt acttttacac ctctaccagt tacacaagtt atatcaccat atccttttaa
    29761 agagcaagtt ttttcgattc ctttaaactc tccatctatg tctatagtac tgttttcttg
    29821 ttgttgtatt accttttgca taattacccc aacgtcttta aatatagagt catttatttt
    29881 ctcactaatt ttattaccat actgatcaac aactagtact ttatttttta cattctccat
    29941 gctttcagaa aaatttgtat tgataagatt aaagccttct tcaaatgtta tatttaaagt
    30001 aactacaccc ttctcaataa cattaaattt atcaagatta gactctatca tatacttttt
    30061 tttagttgtc ttgcttgctt ctgtataaac gctcattata gtatcatagc cagttacacc
    30121 aataaacatt tttgtatatt taacgttagt tttaggtaaa ttacctagtg ggaatttatt
    30181 gtctacaaaa acctgctttg caatatcttc aactaattta tctttaaaat tgtatgacac
    30241 ttcgctttgt aaaagtaaaa agcccatatc tttagctgta aaatctatat tattattgct
    30301 agagtcttta gatctatcta ttatcattcc tctaaaaagt tctttatcat caacatagaa
    30361 acaaactgta cttgctactg gaatatctat ctgcctaaaa ttaatatcac ttgcagattg
    30421 aattatagaa aattctaaag ttcttgatgg tgatctataa tcaccagacc aagtacactt
    30481 atccactaaa tcagttacat tatatatatt tccgtttttt atatgaactt gtattttgat
    30541 tttattaatt ataaatcacc accattttaa ggaattatta atatccaacc attttttatt
    30601 acagatggat ctttaatctt atcttcattt gctttataaa tcttctccca caaatctcca
    30661 ttgccataat atttttttgc tatcttaaaa agggtatctc cttcaacaac cttatgtgtt
    30721 ttttgcttag tatcaaatcc ttttgttagt ggaacatcct tttcagaaga taatttttca
    30781 tcattattaa tgtttacttt ggatatttgt atccttctat attcttttag acttaaggta
    30841 aaataaacat ctcttgaata atcttgttct ctataattaa aatctgcaat tatacattca
    30901 aagtttatat tagtttctgt aatgataaat cttaatatat atccttcttt catccaactt
    30961 tctattaaat ttacacaatc atatggtttt ggaaatccat tataattaca gaaactatat
    31021 tcttgattag ggaaaaaaga ggatagttct atagttttaa gccctaaccc tccaaatact
    31081 gcaatatctc caacacttaa aatattagat gtatttattg ttgcacttcc atttatctca
    31141 aaagagggtg gaataacagg aaacctaaat gtattatttg cttgtcttaa ccatatttcc
    31201 attaaactcc tcctaaaata aaagacactt atttcaataa gtgtctttta agataattac
    31261 tataaatcaa atactgtata ttttcctaca ttttcataag caatttcaat tgtttttggc
    31321 tttgtatctt ttgtaagttc aaatgctatc tcacctttta atgtatctcc tggatgtatt
    31381 gtgtcaaata gtaattctcc atttaattct tgattattta ttgcatttcc aactgtatta
    31441 tcatccgttt ttagtatgta gttttgaagg aaatcacttt caagatctgt atctcctgta
    31501 ttggtgattt caatctcaac tgcatataca ttactatctg attctagttt tctaacattt
    31561 ttaacagata cctttgctcc gtccaaatca acaacttcgc ctattttatg atttttaatt
    31621 tcctctgtat tttgcttagg tttactatct ccattttttg aatcatttgt gctgcatcca
    31681 actaaactta aagataatac aattaatatt gctaatgctg gtattttttt attcataaca
    31741 taaccccctg taaattatat actacaatta tagcacgata atttacaaaa cgcctccaac
    31801 ttttgcaaga gatatttttt tgtttatttt tcttacaatt ttatctatgt cagcctcttc
    31861 tcttacaatt attgtatcag ccaatttatc aagaaataaa cttccatttg aactatttct
    31921 tttgtattga ttggcttctt gtttggttaa aagtttctct ccttcatgtg ccctaattaa
    31981 ataatcattt ctaggaactc tatttatacc aaatgctttt cttggacttt tgccagcagc
    32041 atctgcacct tttccaattt gtaaactagg acctttttta aatatttcta ttgttccctt
    32101 aattggatgt ttaaagaaat ctttaagttt attccaagct tcttttaccc ctttaacttt
    32161 atctttaaaa aatgagtcag caagatctat tacaggttcc agtacaccac taactagatc
    32221 aacaaggcca ttccaaattg attttattac acctactcca ctatcaaata tttgtttaag
    32281 tccatccata gtttgatcag catttcctgt tataagcccc ataataacat taataattcc
    32341 agatataaaa gacataattc catttatgat tccagatact gttgttatta ttgcggttaa
    32401 tgtatttaaa acccatgtaa atgctaaaat tattccgcca acaacaccag ctattgcaat
    32461 tcctatagat ggtaaaagtt gttgtcctat ttgtgatagg aaaggcataa atccttgaaa
    32521 ccaagcttta acaggagcaa gtgcttgagt taaactttgg aaagaaagtt taattccatc
    32581 agtagacttt ttaacactat ccattggttg gcttactttg tttactgtat ctccaacctg
    32641 tttggctgga gtaaaaaaat cagtaatcgc ttttttaacc ttatcaaaac actctttcaa
    32701 attatctaaa tgaggtttta aaggttcaaa tgcttttact aaattatcta tatttgtttt
    32761 aatatttcca gctaatatgt tctttaattc attaaattta tcctttattg aagttattga
    32821 ctccttaaac ttatccttaa aatttactcc agaatcctct aggaccttta atgcaggttt
    32881 aacatcattt aatattttgt tttgcagttc tgtaaattta gtttttccag attcaactcc
    32941 ttgacttata gaatctgtca taggtttaat aaaagtttta aaatcactaa atgaaccttt
    33001 tagatttgct attgtttgtt ttaatccacc tgtatttggt gtagatttcc cagctgcatc
    33061 agctgcttta cttcctggaa tgtttacatt tgttttagta gatattttgg cagttgcttc
    33121 aattggattt tctaagaatg ttcttaaatc tttccactta tctgtaagcc atttagcttt
    33181 atctttaaat aagctgtcag ctatatcagc tatagcttgt ataggagcag ttacaaaatc
    33241 tatgaaacct ttccaaattg attttataat ttttactcca ccatcaaata tttgtttaac
    33301 tccattcatc atctttttac tatcgcctgt gacaatacct ataattaaat taaatactcc
    33361 atttaaaata gatgctagac catttaatac attagttata gtatcaacta cagctttaaa
    33421 ggcagttgcc agagtagata aaatcaaaac taattttgct atatgaaaac ctattacgcc
    33481 aattaaaaca ggtcctagta ccttcattat tacacttcct aagtttaaaa tagacgcaaa
    33541 tagtggacca aatgctgata acaactcttt aaatttttcc cctaattgtt taagtgcagg
    33601 catacaaaaa tttactatag gttttgcaaa ttctgtaaat gattttacta atgaattaac
    33661 cttatttcta aatacttcag atttagcata agcaatagta aatgctactg ccaatgcaat
    33721 tacagctgtt atagtccatc ctacaggacc cattacagct aaaaatactg cacttaatgg
    33781 tttcatgcct attacaaaag ctctgaaagc aacttttgct cttaatatta ttggaacaaa
    33841 aaagccaaat acacctatag cttttgaaac aattaaagac acagccccta tagttaataa
    33901 aagtactcca aatgctccaa ctgaaagcat tacattagtt atgactcttt ttattggttc
    33961 acttagatta ttaaaccaaa cagtcatttt agttaaatta tcaactacag atgctattgc
    34021 tggctttagt tgatagtaaa tagtaattcc agtttcttca agggctgatt ttaaggttgc
    34081 aagtccaccc ttagtattat ccttcattac atcagacatt gtttttaaag caccttttga
    34141 attatctatt tttttcttta aactatcata ttcatttccc acaccatcaa gtaacttttg
    34201 taacgttttt aattgagtct ttccaccaat agcagataag taataatttt tctgctcatc
    34261 agtcatacct ttagttttat ttgcaacctc tttaagtaca tttgacatac ctttaaattt
    34321 accttgatta tcaaatgcac ttacacctaa ctttttcatt gccttacctg cttgacctgc
    34381 acctgttgta agatttacca tgatagagtt taaagcatga cctgcatctg tacccttaac
    34441 tcctctattt gcaagtattc ccatgatagc agttgcttct gaaagtggaa cttttaaatt
    34501 gctaaatgta ccacctgcaa cattaaacgc ttccatcaac tgttgtatac ttgtatttga
    34561 tttagtagat gcatttgcaa ctttatctaa atactcactt aaacctgcac cagctagtac
    34621 ttttccactt ttactgacct tatcaacttc taagcacatg gaactcatgc tatcagttac
    34681 taaatctgat gctagaccga gatccataga ggctgcttca gataaattaa gcactggcat
    34741 taaggcatgc attgactttt gaacatccca tccagcaagc cccatatact gaagtgcatt
    34801 agcagaatca gcagcagttt tagttgtagc ttttccagca tctctagcag ccttagacag
    34861 agcttcaaaa tctcctggtt ttaaggaatt tccaaatgta gccttaactt gagacatagc
    34921 gctttcaaat tctgttccaa ctttagttgc agctgcaaat gcgccaccaa ctccagcagt
    34981 gataagacct cctgctttta aggcgctagt tccaacactt tttaaattac tttgaacatt
    35041 atttaatgct cttgatgctt gggaagatga atcatgtaga gccctagtat ttgctgctac
    35101 tctttttaat gttgaacttg cttcatctct catttttatt attgcttgta ggacctttgc
    35161 attagaactc atatactaaa acaacctccc agcaccagat tttatttctt cttttctttc
    35221 atccaattct tgatccataa atacactaac aatttctttt tcgcctctta gtatacgata
    35281 agaaacggat ggcattatac ctttatactt aaataataag tacatcaagt taacttctcc
    35341 atccgtttta attagttttt tatatcttta gctctttctt tttgtctttt tttatctcct
    35401 tcagcatcta tgccatttac tttattaact tcctcatata aattatcaat ttctcccgct
    35461 aagagtaatt tttttagaag gtctttgggg gttgccaagc ccagattttc tacaacttcc
    35521 atatttctaa acattggaca tgactcaagt attgtattta ccttaagttc ataaacatta
    35581 atattttcta aattaccact atctacatcc attgaattca ttcttatctc atcaaaacgc
    35641 tctggatcaa tagcattaca ttcaatttct aagatatcat ctaatttttt gcaatataat
    35701 tcaaatatac tagacggcat tttcaattta cctgcatcca tatttaataa tttttctact
    35761 gtattcatat aataaaaatc ctcctatttt tctgtaattt tatctaaata tttaaagctt
    35821 tcaaaagtaa atggaacttc tatttctccc atttttcctg cttcccaatc tgctaaagtt
    35881 aagtcatcaa gcatacaacc ataaagagca actctttcag ctccatatga atctgggtct
    35941 tgtaatttac ttattatagt aaatttgaat gcttttcctt cgtttaacat actttcaaca
    36001 tattctccaa atagggaact cactttaaat aaatttacag agccctttcc agatgctcca
    36061 atgagttttt gtccaaccat caattgtcca caaactttaa cctcttcttt ttctaattca
    36121 acttttgcct ctaagccttt tacagccatc atttgcctac catctatcca acattctcca
    36181 aatgtaccag atattattct acttgggtct attttagctt tcaaatttta acctccaaac
    36241 tacatagaga tgtctaaata cacatcttcc atggcatcta ttaatttaat ttttgctttt
    36301 aaaaatacaa ttgtatctgt gttatattct tttatttgtt gttcttccat attagataca
    36361 tcaatatgat tttcttttaa ccatgctctt tgagcatcta aattaatttc tattgttgaa
    36421 cctgggtcaa ttaaaccatc cttttctaat tcttgaagat ataattgtat tgcaacaata
    36481 agtaaacatt tattatcata agtgttttgg gtttttccta tataattttt tacaattatt
    36541 tttttaatat cgttatgaat ttgatctagt gtatcaacca acttaatctt tttgaatata
    36601 tcaccttttg aatcagtaat tgttgtaaaa gatgtaactc ctctagcgat cactatagct
    36661 ccgctttctt ttattaaaat tagctctcca ttatttatcc ttgtatttgc ttcagctctt
    36721 gttaatttag gtatattagt gacttctggt atttctgcat atgttacaga ttgagttgat
    36781 ggagttcctg caataaaacc tgctattctt ggtagaaatt catttgaagt gtatttttta
    36841 ccttcaactt caatgtcagt tgctgtaaag tttattatac cttcataatc tgctgaatta
    36901 cttgcagtaa tagctttaac ctttatttta ttatcttctc tcattttctt aatccacgtt
    36961 ttgattttag gtaaatctcc ttcttcttca tctggcatac aaagataatt aaattcacaa
    37021 gtttctagaa aatctagtgc atcatctatt gattcatctg tatctaatgt ataaactaat
    37081 actttatttg gaggatatac atttcccttc atagccattt ttatatagtc taaattagtg
    37141 gcagaataat tttctggaat gtcctccatt tcttttatct caactagccc tatatttttt
    37201 gtatctttta aaataagtgc tacaatacct ccagaacgtt taacaaaagt ttttcctgct
    37261 tggataaaag aaatattaat ttctggtaaa cccatttaat cacttcctat ctttaaatct
    37321 acattttcca tagttacata tgtttcttta ctaaagtaaa cttcttcaaa ataagatata
    37381 gtcattagaa aagttagttt atgccccaca gaatctttta caattgatgg agaaatattt
    37441 tctatagtta aactcctatc tttaactcta atgtttctat taaatatttt ttctaatcta
    37501 ctttgtatgt catatagatt taatttattt tttctagctt tttggtaata ttcaatatct
    37561 atgaatatct ttttagtatt caaaatttta tttgcaatat tgttagaaac aggtaaaagt
    37621 tgcacaaaaa aacaagcctc ctcaaagcct tgtatattat cctcattaat aaaaatatct
    37681 atatctatga actcattttt taatatttca ttaactgcta ttagaatgtc tctgtttgta
    37741 agcaactcac cacctaccaa tcaaataaat ttgcttttgc acttgtcata ttttcttttg
    37801 ttttttcaat tcccttttca accataaaag aaccaggaat aatgccacca tttttagttg
    37861 catggccatc atttactaaa gttgcatatt caaccttgtc ttcaacaata taagtatcat
    37921 attctggctg actttcttcc catccatttt ttaacttttc agttctcaca ggcgtttcat
    37981 ctcttatatt ttctgctagt tcttgcccag ttttatttgt aaacttcttt atttttctat
    38041 taaagttaga agcatttaga cctaaatcta aggctaatgc ttcaaagact cctaaattat
    38101 ctattcccat tatattctct ccgcttctgt aataggtatt tccatatggc ttggatatat
    38161 attcgcttta gaaaccttaa atacagcttc ttctccatga ctataagttg catttatagt
    38221 gtcgcctgtt tttatatcta cttctggtct acaaaataac tttctattta tgactatagc
    38281 tccaatatcg ccgcttataa tggcctcttc ttttcttgaa attgcacatg ggatattctc
    38341 agctataata atattactaa aatcattaga acatgtttct tgattccaac cttttgactt
    38401 tctgatgata gtcattttat caagataggt catttctaat atatctgctt ctgtcatttg
    38461 atcacccctt agtacatatt aacaatacaa aatggactta gaaatttttt atcagtatca
    38521 gttaaaaaag atgatgaact gatgctattt tcagcagttt gtaatgttaa agcattatat
    38581 tctatcttag tgtcacctct agttatagct tttaagcttt tttctagatt atcaagtcca
    38641 ctatttttct taagttcttc aaatcttgaa gataatattg atattacttt ttcttctaca
    38701 aacccttcta gtgctatact tagttcctgc ctagaacaat attctaaaac catctcactt
    38761 attttattta tataaaggat tattaatttg tcatatctat cattttctat tcctaaaact
    38821 aatttaacat tatcaagcat tgtactcatc tagtagttga attaattcct ctttagtttt
    38881 agttgaatat ccctttatat tcttgccttt agcaatttcc ataagctctg gtttagatag
    38941 attaaataaa tcattagagg ttgatttaac tttttcttct tttttctcaa tattctcttt
    39001 agtcacttct tctgaatata ttttaaatcc ttttgaaatc aatttatctc tcgaattttc
    39061 atcttcaaca actttaatta tgttattttt tatcaatgca aacatttaaa acaactcctt
    39121 attcttgtgc agctttagct tgtctcacag atgctctaca aagttttaaa tattcatcca
    39181 atatccacaa atcgtgatat tttctatagt ctatttgata agcatctgct gtttggtttt
    39241 gatctggagt aaatattctt ggcttatcag tcttagatac tgcaattgga gcagatgctg
    39301 gagttattaa ccagtttata tctaaagcct ttgctccttc agtaaaccca ccctctgttt
    39361 gtccagatgt tttaccatct ctaaatacgt attctgtttt cattcttttt gatggaactt
    39421 ttattatagg tacattgtct atagacttta caattctttt tatatctcca tcgccaaatt
    39481 caatatcatc aagttgagat gattgttcta atatgcttgc aattatgcta gacatactaa
    39541 taataagagg tatatctcca gataaatctt gtattgatgc aagttgattt ttcaattcac
    39601 ttaatattgt agttactgta gcttcgtatg aatattttac acgctcatct actttagctg
    39661 tttcagaaaa tatctttgag tatctatatg catctatttc tggtataaca tgttgctttt
    39721 gaaataatcc cattacagaa gatgcattta taacgaagtt actttcattt acatccattg
    39781 aatctaacat aaaagtccta cctctatcca tctccatttt cattgtttcg tactcaagtg
    39841 taactgcgcc ttgcgtatat cctttagatc tgtcataatc acctaagcca tccattgtta
    39901 cttttggtac ttttacctct cttcctccat tatatataac ttgaccagca tttttctcca
    39961 tccatccagt agttgagcca actgttacct gcatatctag ttcttgttga aaaagtgtta
    40021 catattgtaa tacattagcc atttataaaa tcatcctttc tttttttata ctaaagacta
    40081 tttagccctt gctttatttg ttttctaatt tctgatagcg aacctgcact atctttgcca
    40141 ggtggaacgt atgaaccatc tttaattctt tcatctacct ttgactgcac atattgcttc
    40201 attgaatttt caaatatctc tatatttgca tttgttttat catcatcatc agataataga
    40261 tattcaatta agtttgtagg tattttcttt tcaactaaaa tatctttata tttagaaact
    40321 ttttctgcgt actgcttttc tttttttagt ttgtctaggc tttctcttaa ttcccttatt
    40381 gctttttgct cttctgtttc atttgctcca gttcttttta gaacttcagc acttataagt
    40441 gtctgcatat cttttttctt aaagttttca agtgcttcgc tgtgatattt atttttttca
    40501 ttatctataa tagctttaaa gtttgcatca ttattaatta attctctaaa ctcttctatt
    40561 gtaagctttt tatcagatgc agatttgaat aaatctgttg attgcagcaa tgaatctatt
    40621 tctgcatcat cttctatttc ttttagtaat tccaacactt ctttttttaa catgtataaa
    40681 gttctccttt ttgaaaattt aattcaaaca ttctatttta tccataacct ttactatttt
    40741 aggaaattga attgcaaaga agtcaatcat ttcttcgttt gtagcccaag aactacaact
    40801 cttcaatcca ctttcactaa gaaatgcatg aattatttca tgcctagcaa cttcattttt
    40861 atatacactt aaatctttca atgaaccttc ttcagaattt attttctcta taacaatttc
    40921 cttaacagag tgatcacaat atccagctct ttcctttaat agcacatctt cgctttcaag
    40981 tttctctatc actagatatt ctgttcctaa tatttcaact ttcatatttc acctcatatt
    41041 ttttagacat aataaaaaca cctattaaat taattaatac atgttaaaca ggtatatatt
    41101 gacatctaca gtgacagtgt cttggcaaag agggcctatc ttcattgaca taaaatatca
    41161 ctccattatg catagaacaa tcactacatg tgcgagaatc taaatctgca caatacatta
    41221 cttttataat cccttgaacc ttgaaaaaat actcatttac ttcactttga catctggcaa
    41281 cttcattttc tattagtctg ctggtttgtc ctgcacctgt tgaatattta tctcttattg
    41341 ctttttctat cttatttaca gaagtttttc catttagaaa actctttatt tcttttttta
    41401 aggactcttc tatacctttt ttattcttcc agagtctatc gctccaatgt tttccatcaa
    41461 ccttattatt cactatctta tttataactt tattgtttat attactaaca tcttttatct
    41521 tcagtattac tcctaataaa attatcaaat ttgaatattt attcttagat gaatcagata
    41581 aaatagtttc tatatccttg acttcttcat cataactatt agaatactca tttataattt
    41641 tattaagttt tgaccttaga tttttcttat ctttattatt aatatttaag tattcattaa
    41701 taatatcgtg tgttagcaat atctttgcta tttctaataa aagtttatcc ctattttctc
    41761 tttgatactt ataaaatggt tttgtcctat tttcagcaga tttatatata tcttcagtca
    41821 atttagttat aatcttttga ttcatctaat aaaatctctc cctctgaaac tatttcctgt
    41881 tcttttttag ctttttctat ttcagcatga ggattatcaa tgaaacttag ttgagataag
    41941 ccagtttcag tagataattt atctccaagt tggcttaata tatttgctac aactaaatca
    42001 tcacgtggta tcttaggggt aaatttagcc ttaatatctt tataatcata gtcaaaacca
    42061 tattttttat taagccaaat gcacaataat ttgtacctat taaataaagt atctgcaata
    42121 ctatcattat tttggctaca aatccattct gtactgatta atcttgctat aactgctaga
    42181 cttgatgtat tactttgcat tttttcattg tgatttatat gagatgtaat ttcatacata
    42241 ttttctttta ttgaagatag tgtattttga acaaatgtat cattcatatc tttaataagc
    42301 cactctattt ttccatctcc attaacttga agtattccaa gttctttcat ccttggcaaa
    42361 tcatcttctt taatattaca tccagaaaag gttaaataag catttctaaa atcactaatt
    42421 tcattactta tatcacttaa attagtttca taagcatctt gtaacccttt tatatcatta
    42481 aatattgtat tttttcctag ttcttcactt cttttgcaaa ttgatattgg aacatttcca
    42541 tcaaatatat tgtttactgt aggactttca acttctttga aatttgaatc aaaatgatat
    42601 atatattctt tgtcatatac atctacatac gtgtcatctt cgaaaccttt ggtaaactct
    42661 ctaataaagc atactatttc tccattttca ttttcataat gacagccatc tgttggcttg
    42721 attatcttta cttgtatttc atcatctttt acatagtaaa gttcatagca aagcccaaat
    42781 aatagtgcat acctcaataa atctgagtca tgttttttac tccaatggca agtatagtat
    42841 tctaaatcat ttataatctt ctcatcacca gatctacttg tataggtaat cttatttgca
    42901 agagaataag cgacttcttc attaataaac ttttttaaga aattagtgtt tatcttatta
    42961 ttagatctct tggtaaccat tttatagttg ctaatagcat ccgtttcacc tttataatac
    43021 ttatacatag tatcatattc ggatttatta gagttccaat cgtcatatat gtcttttatt
    43081 aagtctaaat ctaattccat taacaacaca tcctatcctg tagactctat aaattttaaa
    43141 acttttattg gaatatcttg ctttgaaata taacttttac tccttgctct atgagtatat
    43201 gcaaaatatc ctttttcatc ttttcctatt ccaacattga acttctttcc ttttttagtt
    43261 aaagtttttt gaacttcttc aaactttgat ctattagcca ataaattacc tcccaactaa
    43321 aaacctaata attttctatc aaagatattt actgtctcag aaacttttat gttgttaatt
    43381 cgattaataa attccgatga tatatccgga gcatcatcat gtagagagta tttttgtcct
    43441 gtaaaatcaa gtatttgctg tataaattcc ttatcttctt ctgcaaaaat catttggcct
    43501 ttgcttaaaa caggtattaa agtagagatt ttatcatctt tatttttctt ttgttgctca
    43561 ttaatgattt ctatatctct ataactaaga acattatcat tttttatttt aaactctaat
    43621 tggttggcat ctgcgccatt aaatgtattt ttttctatat aaatatgtgt tatatctgaa
    43681 tacactttta ataaataaat catatggtct atatatttat caaaatcagt tctagcattt
    43741 attttagcta gttctgccaa tcttccatat agtaaaccat tttctgattc acttccaaca
    43801 agaaaagcgc tatagtcatg tttcgaccca cccgcagatg ctggatcaca taacagcatt
    43861 gtctttttaa aagtatgcga ttcaatttct tctctagttt ctgttctaac agttttaaac
    43921 catttttctc ctatagaatt gacatcattt tgcacttcct gcttaaaaga tgttggattt
    43981 tcataataac ttagagccat atctaaacaa tcccaaaaac ttggccataa tatagggaat
    44041 ttcatttctt tttcattttc ccagtaaaat tctttagcat cttcaagatg atttgtattt
    44101 ttaaaatcaa ataagatctc tttgaactta agccataaac cagaattaaa taattcatct
    44161 acattatcaa ctaaaactcc tttttcgcat ttaaacttcc aagttggtaa attttttagt
    44221 ctactataaa aacattcttt gtgttgtaag gttcctaatg ctataaaagt tgttcctctt
    44281 tttatagttt taccatttct tatgactggt ttttgagcag catattttac atcatcagaa
    44341 aatcttttaa attttttctc tctagcatct tctgttctaa cattttcttc tgactgataa
    44401 tcatcaagta tgataagatc tggacggtta ttattgtatt ttcttcctct catgggtgat
    44461 gaagatgata tagcttcaat aaaagtttta tttgttagct ctagttgagt actattacaa
    44521 atgtattttc tatcattatc atttaaaagg acaccaaatg cttttttaat atactcattt
    44581 tcaagtaatg cattttttat atcttttaca aatttctctg ctgtagatcc tatgtcagag
    44641 caaatcaaag tatatgtctt atgtttatag caatgcgacc atacagttgt cgctaaatct
    44701 ccaaatgcac ttttacctgt ccctcttgga taaattcttc ctaattgctg tgaaccatct
    44761 ccaattatag attcttgtat atcttcccat agctcatgat gaactttagc tataggagca
    44821 gcagcattat cttcttttgg taaatatgtg tcttgcataa aatacataca gaaaaattct
    44881 aggtttattt ttccaagttg ataagcgaga ccattaactc cccaaagatt gtttgaattg
    44941 gcttttataa tctcatttgc ttctttttca gcatctttta tagatgctcc atttcttata
    45001 aaagtctttg ctagataact aaatattaat tgtatatttc tttctagatt atccactcgt
    45061 atttacttcc tcttacttct tctatttttt taggctcagt gagtaatgca attaataaaa
    45121 taaacaatgt aaatttttct ctaataaaat caccctcttt gaaatacatc tctggttttt
    45181 aaattttgct agaaaatttc tagaactcat tttccataaa aaataatttt ttcaaaatag
    45241 aaggacaccc cctattaat
//
LOCUS       DAELSY010000013        44609 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.013, whole genome shotgun sequence.
ACCESSION   DAELSY010000013 DAELSY010000000
VERSION     DAELSY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.013"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            56..1114
                     /locus_tag="KPG09_000641"
     CDS             56..1114
                     /locus_tag="KPG09_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG0965268.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            1365..3509
                     /gene="fdhF"
                     /locus_tag="KPG09_000642"
     CDS             1365..3509
                     /gene="fdhF"
                     /locus_tag="KPG09_000642"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1779..1781,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBG0965269.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKTWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            3558..4391
                     /gene="fdhD"
                     /locus_tag="KPG09_000643"
     CDS             3558..4391
                     /gene="fdhD"
                     /locus_tag="KPG09_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBG0965270.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCASVKSNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            4402..4575
                     /locus_tag="KPG09_000644"
     CDS             4402..4575
                     /locus_tag="KPG09_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965271.1"
                     /translation="MCTKKEEQKCSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            4633..5184
                     /locus_tag="KPG09_000645"
     CDS             4633..5184
                     /locus_tag="KPG09_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0965272.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            5209..6585
                     /locus_tag="KPG09_000646"
     CDS             5209..6585
                     /locus_tag="KPG09_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG0965273.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            6585..7100
                     /locus_tag="KPG09_000647"
     CDS             6585..7100
                     /locus_tag="KPG09_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0965274.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(7178..8461)
                     /locus_tag="KPG09_000648"
     CDS             complement(7178..8461)
                     /locus_tag="KPG09_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBG0965275.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSHSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            8649..9071
                     /locus_tag="KPG09_000649"
     CDS             8649..9071
                     /locus_tag="KPG09_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBG0965276.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSEKNGENIIRDILDGNISSECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            9173..10111
                     /locus_tag="KPG09_000650"
     CDS             9173..10111
                     /locus_tag="KPG09_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBG0965277.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            10297..12321
                     /gene="ligA"
                     /locus_tag="KPG09_000651"
     CDS             10297..12321
                     /gene="ligA"
                     /locus_tag="KPG09_000651"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBG0965278.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            12481..13830
                     /gene="rph"
                     /locus_tag="KPG09_000652"
     CDS             12481..13830
                     /gene="rph"
                     /locus_tag="KPG09_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBG0965279.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEIADEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARAIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            13830..14300
                     /locus_tag="KPG09_000653"
     CDS             13830..14300
                     /locus_tag="KPG09_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0965280.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            14494..15780
                     /locus_tag="KPG09_000654"
     CDS             14494..15780
                     /locus_tag="KPG09_000654"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBG0965281.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            15929..16513
                     /gene="clpP"
                     /locus_tag="KPG09_000655"
     CDS             15929..16513
                     /gene="clpP"
                     /locus_tag="KPG09_000655"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG0965282.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            16532..17782
                     /gene="clpX"
                     /locus_tag="KPG09_000656"
     CDS             16532..17782
                     /gene="clpX"
                     /locus_tag="KPG09_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBG0965283.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(17853..18380)
                     /locus_tag="KPG09_000657"
     CDS             complement(17853..18380)
                     /locus_tag="KPG09_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG0965284.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(18368..18880)
                     /locus_tag="KPG09_000658"
     CDS             complement(18368..18880)
                     /locus_tag="KPG09_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG0965285.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            19060..21423
                     /gene="lon"
                     /locus_tag="KPG09_000659"
     CDS             19060..21423
                     /gene="lon"
                     /locus_tag="KPG09_000659"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBG0965286.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFNSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     ARKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTAVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            21413..22024
                     /locus_tag="KPG09_000660"
     CDS             21413..22024
                     /locus_tag="KPG09_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBG0965287.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(22289..23659)
                     /locus_tag="KPG09_000661"
     CDS             complement(22289..23659)
                     /locus_tag="KPG09_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0965288.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYALGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDISGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(23834..23949)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            24237..25316
                     /locus_tag="KPG09_000662"
     CDS             24237..25316
                     /locus_tag="KPG09_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0965289.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKATEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            25708..26388
                     /locus_tag="KPG09_000663"
     CDS             25708..26388
                     /locus_tag="KPG09_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG0965290.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            26381..27760
                     /locus_tag="KPG09_000664"
     CDS             26381..27760
                     /locus_tag="KPG09_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG0965291.1"
                     /translation="MINIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGVLLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            27931..29016
                     /locus_tag="KPG09_000665"
     CDS             27931..29016
                     /locus_tag="KPG09_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG0965292.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            29070..29429
                     /locus_tag="KPG09_000666"
     CDS             29070..29429
                     /locus_tag="KPG09_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBG0965293.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            29482..30354
                     /locus_tag="KPG09_000667"
     CDS             29482..30354
                     /locus_tag="KPG09_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBG0965294.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            30355..30798
                     /locus_tag="KPG09_000668"
     CDS             30355..30798
                     /locus_tag="KPG09_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965295.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYINQNKLVDKSKHTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            30813..31397
                     /locus_tag="KPG09_000669"
     CDS             30813..31397
                     /locus_tag="KPG09_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965296.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            31650..32687
                     /locus_tag="KPG09_000670"
     CDS             31650..32687
                     /locus_tag="KPG09_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG0965297.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            32768..33133
                     /locus_tag="KPG09_000671"
     CDS             32768..33133
                     /locus_tag="KPG09_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965298.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINVAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            33138..33455
                     /locus_tag="KPG09_000672"
     CDS             33138..33455
                     /locus_tag="KPG09_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965299.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            33503..35107
                     /locus_tag="KPG09_000673"
     CDS             33503..35107
                     /locus_tag="KPG09_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965300.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVVLKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYINVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            35231..36859
                     /locus_tag="KPG09_000674"
     CDS             35231..36859
                     /locus_tag="KPG09_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG0965301.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIAKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            37124..38473
                     /locus_tag="KPG09_000675"
     CDS             37124..38473
                     /locus_tag="KPG09_000675"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBG0965302.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            38629..39708
                     /locus_tag="KPG09_000676"
     CDS             38629..39708
                     /locus_tag="KPG09_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG0965303.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            40053..40961
                     /locus_tag="KPG09_000677"
     CDS             40053..40961
                     /locus_tag="KPG09_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG0965304.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGVNIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            40966..43335
                     /locus_tag="KPG09_000678"
     CDS             40966..43335
                     /locus_tag="KPG09_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBG0965305.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRLTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPKDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            43660..44463
                     /gene="proC"
                     /locus_tag="KPG09_000679"
     CDS             43660..44463
                     /gene="proC"
                     /locus_tag="KPG09_000679"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG0965306.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGINTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT        18308 a         4773 c         8082 g        13446 t
ORIGIN      
        1 tatactaata atatagtatt tagtataaat cttgagcaaa ggatggttat ttctaatgga
       61 tagactaaag aatattagaa agagtttact acagtataca tttttatttt tgttaatagg
      121 tataacaatt tacctagtgt ttaaaacatt ggatattaaa atgctatcaa atgttatggt
      181 tatggtagat aagaagtttt tattcatagg tgctttggca ataatgtgtc acataatgct
      241 tgaaggagta gtaatgaaga taattataga aagcactcac aaagttaata ttagatttat
      301 tggttttaag ctagccatta tgggatttta ttataattta ataactcctt ttgcttcagg
      361 aagccaacca gtacagatat atgtacttaa aaaatgtaaa atgccactta gtaaggcaag
      421 tgcagttgtt accaataagt ctattatttt ccaaatagtg gttacttttt attgtacaat
      481 acttgtatta atgaattttg ctatgttaga aaaacaaatg aaaatgatta tgcctctagt
      541 gtttctaggg atagccatta attcatttgt cttaattatg ataatattag ttattttaaa
      601 ccctaaaaaa ataaaatatt ttatgagaat tactataaag catttatcaa aaattaagtt
      661 tttaaaattt ttagataata agattgaatc catagaaagt tttatagatg attatagtaa
      721 agcaataaat ttttttgcaa agaataaaaa ggtattaatt tcaactataa tagtaacttt
      781 tattcaatta agtgcttact ttagtgtttc attttggata tataaagcat ttaatttaca
      841 aggttatagc tatatttata tactgacatt acaagcattt ttatatatgg caatatctcc
      901 aataccaaca cctgggaatg taggagctaa tgaattagcc tttttcacta tattcaaatc
      961 agtattccct caacctttga tgggatatgc agtattttta tatggtggat ttatgtacta
     1021 tttaattctg attggaagtg gaatatttac tgtaattact cactatagaa tgaaaaatag
     1081 aattggcaga aatattgttt taagtgaaaa ctaaataagt ttaaagaata tatattgagt
     1141 atattaaaaa atatgtcata agaactagaa tattttaatt aaatggttga aatataaaga
     1201 ttattatgac atattttttt tgaaatttaa gaggaattgt gagaaaattg ttgaatttaa
     1261 tagatagtat tgcaaaatat ttatgatttt gcatattata aaaacactca ttatctttaa
     1321 gataaatttt acttatcagg ggataaatct aaggggggaa ttgtatggag aaaaaaattt
     1381 taacagtttg tccatactgt ggagctggat gccaattata tctagtagta aaagataata
     1441 agatattaag agctgaaccg gctaatggta gaacaaatca agaaagtttg tgtttaaaag
     1501 gtcgttatgg atgggatttt ctaaatgacc ctaaaattct aacacctagg ctaaaaaagc
     1561 caatgataag aaaacaaggt gtactagaag aggttgaatg ggatgaggct ataaactata
     1621 cagcttcaag actaaatgaa attaaagaaa aatatggacc tgattctatt atgggaacag
     1681 gttctgcaag aggacctggt aatgaagcaa actatataat gcaaaaattt atgagagcta
     1741 caataggaac taataatgtt gaccattgtg ctcgtgtatg acatgcacca tctgtagccg
     1801 gtctggctta ctcattaggt agtggtgcaa tgtctaattc tataccagag atagaaaatg
     1861 cagatgtttt atttatattt ggatataatg gagcagattc acatccaata gtggcaaata
     1921 gaatagtaaa agctaaaaaa aatggtgcta aattaattgt tactgaccct agagtaactg
     1981 aatctgcaag aattgctgat atacatctac ctataaaagg tggaacaaac atggttttag
     2041 taaatgcttt tggaaatgta ttaatagaag aaggtcttta caataaagaa tttgttcaaa
     2101 atcatacaca aggatttgat gagtataaag aaattgttaa accatataca gctaaatatg
     2161 ctgaaaagat tacaggaatc ccagaagagc ttataagaaa agctatgaga gaatatgcaa
     2221 aagggaaaaa agctatgata ctttatggaa tgggtgtctg tcaatttggt caagctgttg
     2281 atgtagttaa gggacttgca tcaattgctc tacttacagg taattttggt agagaaagcg
     2341 ttggaatagg acctgtacgt ggacaaaata atgttcaagg agcttgtgat atgggagctc
     2401 ttcctaatgt atatcctggc tatcaaaatg taactgatga taagataaga gaaaagtttg
     2461 aaaaaacttg gggggtaaaa ttatctccta ataatggtta tagtttaact caagttccag
     2521 acttagtact aaaagagaaa aagcttaaag cttattatat atttggagaa gacccagtgc
     2581 aaagtgaccc agatgcatca gaagttagag aagcattaga tgaattggaa tttgtcattg
     2641 ttcaagatat atttatgaat aagacagcac ttcacgctga tgttatactt ccagctactt
     2701 cttggggaga acatgaggga gtatatacat gtgcagatag aggattccaa ttgatgagaa
     2761 aagctataga gccacaagga gatgtaaaac cagactggca aattataagc gaaatttcta
     2821 cagcaatggg atatcctatg aattataaaa atactaagga aatatgggac gaacttagac
     2881 aattatgtcc aagtttctta ggtgctactt atgaaaagat agaaactcaa ggatgcgttc
     2941 aatggccttg taagagtgaa agtatggaag ataaaggaac tatgtatctg tatgaaggtc
     3001 aaaagttttc tactcctaat ggtaaaggta atttgttcgc tgctgaatgg agacccccta
     3061 tggaagttga agatgatgaa tacccattta gtttatgtac tgtaagagaa gttggacact
     3121 attcagtaag aacaatgact ggtaattgta gaacacttag ttcattagaa gatgaaccag
     3181 gacgtgttca aataaattct aatgatgctg aaaaattagg aattgaagat gatgaattag
     3241 ttagaattag ttcaagaaga ggaagtgtaa taacaagagc aacagttact gatagagtta
     3301 aggaaggtgc aacatatatg acttatcagt ggtgggttgg agcttgcaat gaacttacaa
     3361 tagcaaattt agaccctata tctaaaacac cagagtataa atattgtgct gttaaacttg
     3421 aaaaacttga agaccaagaa ttagcagaaa aatgtgtaag agaagagtat caaagtttga
     3481 aagacaaaat gacagctaca aatatctaat gatttattag atttaagatt tataaaaggc
     3541 agaaaggcta atattttatg aacaatatat actcaaaaac taaattacaa gaaaatagta
     3601 aggataaagt ttttgaaaat agttatagtc tagtggacta caaatattta aattctttta
     3661 atgtagaagt aagcaaaata aatgaacatg aagtatcaat agataatgaa gaaataattg
     3721 cagaatatcc acttagtttt attttaaatg ataaatatgt aaatacattt ttatgtaccc
     3781 catataattt agaggaactt atagtaggat tcttgaaaac aaaaggatac atagaaaaca
     3841 aaaaaagctt attaaaattt gagataaatg agagtgaacg ttttgcaaaa gtaactattc
     3901 aagagaaaca aaaaaatcaa gatgagcaaa ttatattttt aaattcacta gactatataa
     3961 catgtgcttc tgtaaagagc aatataaaaa taaattatga tacgatttac agtataatgg
     4021 atagaaattt aaattcatca gaattattta aaaatactgg tggggtacat agcgtatctg
     4081 tatttaattt tgataaaaaa atagtcactt gtgaagatgt agcaaggcat aatgcaatgg
     4141 ataaggctat tggattttgt gttttaaatg acataacatt aaaagataaa attgtggctg
     4201 taagtggaag aatatcattg gaaatgatat taaaagcagc tatgactcaa attcctattg
     4261 tagtatcaaa atcagcaccg actaatttat caatagagct ttctaaaaaa ttaaatataa
     4321 cgcttattgg atttgtaaga ggaagacgga tgaatattta tgcaaatcca cagagagtta
     4381 tcaaaaagtg agaataagta tatgtgtact aaaaaagaag aacaaaaatg ttcaaagtgt
     4441 gaaatctgtg gagtattaat tgacgatact gataaggtgt ataataacac atacaatttg
     4501 tgcaaaaaat gtaaacgaaa tataagtact gagaaaatag tgaaatatat aaatatatat
     4561 gatgaaagta agtagacata tgatgaaagt aactagaggg ttggaaatat acaaattgaa
     4621 aggtgggata atatgaaaaa taaaataggc tcttttgtaa tagcggaccc aaacaaatgt
     4681 acaggttgta gagcttgtga agtagcttgt tttacaatgc ataatcagca taataatgtg
     4741 ggttatacag ttggaacagt tgatatacct gttataccta gactttattt agtaaaaggt
     4801 gacaatttct gtatgccaat acagtgtaga cattgtgaag atgctccttg tttaaactca
     4861 tgtccacaaa aagctatagt caaagaaaac aatattatgt ctgtaaatga agaaaagtgt
     4921 ataggatgta aaacttgtct gctagcatgt ccttttggag ctatagactt acttccacaa
     4981 tatgaagatg gtaaagaagt agaacaagta attttagatg aaaataagaa gatagcttat
     5041 aaatgtgact tatgtaaaga caatgaaaaa atagcttgta taagtgcatg tcctcaggaa
     5101 gcattaaaac ttgttactcc aatagatgac aaaaaagcta agaatagaca agctgcactt
     5161 agtttattgt taacaaataa ataaaggata caggggtagg tgacgtttat gataataaat
     5221 atagataagg atttatgtac tggatgtaga gaatgttcaa aggtctgtcc agtaaatgct
     5281 attgaaggtg aagagggaaa gccacaagag ataaatttag atagatgtgt tatgtgcggt
     5341 caatgtgttc aaacttgtaa atcttatgcc tcagttatag atgaaggatt tgagttttta
     5401 caagagaaaa aacaagaaag agagatacca gagtctataa atgaacctat atttgcagca
     5461 tacaatgtat gtgatataga taaggtaaag aaagctttaa gtgacccaaa tgtattcact
     5521 atggtccaat gtgcaccagc agttagagtt gctttaggag aagatttcgg atattattta
     5581 ggatacttag gtgctggaaa aatggcagca gcacttagag ctttaaattt tgatagagtc
     5641 tacgatacta attttgcagc agatttaact ataatggaag aaggtagtga attaattaaa
     5701 agagtgacag aaggtggaac tcttccaatg tttacatctt gttgtccagc ttgggtaaaa
     5761 ttcatggaaa aaaattatcc taaattaact aatcatttat cttcttgtaa atcacctcag
     5821 cagatgggtg gtgcaatatt taaaacttat ggtgcagaga taaataatgt agatgcatca
     5881 aagatattta gtgtttctat aatgccatgt acatgtaaaa aatatgaatg tgatagagaa
     5941 gaaatggatt ctagtggata tagagatgta gatgtagtat taactactag agaactagct
     6001 tatttaataa aagacatggg aattgatttt aaaaatttaa gagaagaaaa atttgatagt
     6061 cctttaggta gttatagtgg agctggaact atctttggtg ttactggtgg ggttatggaa
     6121 gctgctataa gaacaggata tgagcttata actggagaaa gtattcctga tgtagaagta
     6181 aaacaagtaa gaggagaaaa tggttttaga aagtcaacta ttaaaatagg agacttagac
     6241 ttaagagttg gagtagtaag tggattaaaa aatgtaatac cagtgcttga agacttggaa
     6301 aaaggaaaat tagatgtaga ttttattgaa gttatgactt gtcctgttgg gtgtgtaagt
     6361 ggtggaggtc aaccaaaaat tttactagaa gaatataagg aattggctta tgaaaataga
     6421 acaaaagcaa catacgtgca tgatgaacat ttagcaatta gaaaatctca tgagaatcct
     6481 gatattaaaa aactgtatga tgaatatctg gtagaaccat taggagaaaa atctcatcat
     6541 ctattacaca ctacatattg tataggaaag gaagtggcta agtgatgaac tattttgtta
     6601 tagcagaccc tgataaatgc ataggatgta gaacttgtat gataggatgt gtagttgcac
     6661 attcagatga agatattttt taccaaaatc cagatgaaat aaattttaat ccaaaacttt
     6721 ctgtcataaa gacaaaagaa gtaagtgctc caatacagtg tagacattgt gaagatgctc
     6781 cttgtgcaaa ggcatgtcca aatggtggta tagttagggt tgggaacaca ataaaaataa
     6841 atgaagaaaa ttgtatagga tgtaagactt gtatgttagc atgtcctata ggtgcaatag
     6901 acatagttac attaaaagat gtagatgaag gaaagctttg ctttagagaa agaatgacag
     6961 caaataagtg tgatttttgt atagaatcgc cagaagggcc tgcatgtgta aatgtttgtc
     7021 caactaaggc atttactata gttaaggatg aggatataga tagtaaagtt aaaaataaga
     7081 gaaaattagc tatattgtaa tataaaaaag atggagtttt atttatgatt aaaagctcca
     7141 tcttttttat tataatattt atatatctaa aaatcaatta ttgcattagt ttatctaatc
     7201 tttctttatt caattttttc attcctatag aagttataat aagtaaagta taggcaatta
     7261 taactgataa aattataata gaccaagaaa atctgtatcc aaaattatca actaacaatc
     7321 caaataatgt tgaacctaat gcaaatccca ctgcaaaaat taattgtacg attccatata
     7381 tgctaccaaa ctccttttga ccaaagaaag agccagttaa ataagcagga ccaatcatat
     7441 aagcaaatac agaaagacct ttcaaaatgg caaatacaaa tgcaaaagta gaagatttat
     7501 ctaccaatag taatgataaa gttgctataa gaaccaatac aaaagatata ataagtggct
     7561 ttgtttgacc gaatttatca aacaataatc ctccaaataa attaccaatg agagcaaata
     7621 aagcaaatgt ggagcctact gttcctatta gaataggatt tagataattt ctaagataag
     7681 ttggatattg tacggataat gatgaaacat atataccaac aaatataaat ccaatagtca
     7741 taatccaaaa atatttaatt tttttagctt cttttaatgt ataaccccat actgaaggag
     7801 aaattgtatc agatgctttc tcttttttac ctttagattt taccttttca ttaatatctt
     7861 taggcatcct gagtatgaat aatattacag gtataccaac tattaatgct ataattccaa
     7921 atattagata agaatgactg tatccttgag ttgctaataa gcgaactgca agtggttgta
     7981 gaaatatact accaatagaa ccaccagcaa aagcaaagcc caaagctttt cctttgcttt
     8041 cttcatcaaa ccaagcactt attaaaacag gtattcctat agcagatata attgtagcgc
     8101 caatttgaac aatacatgat acagtgtaaa attgccatag ttcagtacat atagaaaaag
     8161 tagcaaaagc aatactagaa attatacaac ctaataaata tagtatctta atattgactt
     8221 tagaaaataa tacacctata aaaggtgatg caattgctga ggcaatagta ccaatagtga
     8281 atattagaga aaaagaagct aaactaaagc cttctccttc tacaacataa cttataaatt
     8341 gaggctgtat acttgctaca atactaaatg gaacagcttg tattaacata caaccaagta
     8401 ctataaccca agcatttgaa aagtttttaa tgctaaattt attgtttagt aatgtattca
     8461 tggtatctct cctttaggtt atgatttgta gatattaatc taattattct aattttatat
     8521 attagttttg gatagcctaa ctatttaaat gtaatttggg tattaatata aaaattaact
     8581 ataaaaattg attattttaa atgtatgggg taaattaaag tatataaata aatgaaagga
     8641 tggataaaat gtcatttgaa aaaataaata ataataacct agataaaata cacaatagga
     8701 agtgtattat gcttgtaaac tttaataaaa aagaattatc attaattaaa aacgtaagta
     8761 actttgctgg aataagagac catataatct taagtgaaaa aaacggagag aatataataa
     8821 gagacatttt agatggaaat atatcaagtg aatgtgaaaa tgggattaag agtaagtctg
     8881 taatatttaa taacactcca agcacacaag taaatgcatt tttagaaggt ctaaaaaaag
     8941 tgagaataaa tagacctttt acagctatgg taacagagac atctattgat tggacattga
     9001 ataatttaat agaaaattta gttcaagaac gtaatgctat gtctttagga aaaacaattg
     9061 aacataagta aatttaagat atcttatagt aattttaact aatgtacatt gagtatcata
     9121 ttatatttta gaatataaaa ttttaaatgt gggtagatag aggagatttg agatgaaagt
     9181 tttatttaca ataaattatg gaaaagaaaa atttgataaa ataagagaat taggctatga
     9241 tgtcatacat tactctgaag atagtattga aaataatgag gatgtaaata cagcagatgt
     9301 actagttaca tacaatccat ttgagagatt agatatttct aagatgaaaa atttacaata
     9361 tatacagaca actagtgtag gtatagacca acttccaaaa gaagaggtat tgaagagaaa
     9421 tattaagata gctaataata gaggcggata tagtgtacct attggtgaaa gtattgtatt
     9481 gtatatttta gaaatatata aaaatagtgc aaaatttttt aaacaacaac aaaataaaga
     9541 atggaagatg gatttttctg tatcagaatt gtatggacaa agagttggat ttttaggaac
     9601 aggtactata tcttcagaag cagcaaaacg attaaaagct tttggtgtag aaatatgggg
     9661 tgtcaatact gatggaagta gtaaaaaata ctttgataag tgttttgcta ctgaagatat
     9721 gaatattgta tttgaagaat gtgatatagt agttgtagca atgccttcta caaaaagtac
     9781 agatggaata gtaaacaaag atatgtttaa acttatgaaa gatggttctg tatttataaa
     9841 tgtaggtaga ggaaatacag ttaatcagaa agatttagaa gaatgtgtag agaaatttag
     9901 aggggtagca ctagacgtat ttgaaagtga acctttaaac aaggacaata agttgtggga
     9961 gtgcgaaaat gtcatagtaa ctcctcataa ttcttgggtg tcagataaaa acaaagagag
    10021 aacatttaat atggtttata ataatttgaa acactatata atggagaata aacctgtaga
    10081 taatgttgta gacatatcaa gaggctatta aaaataaaaa attttgataa aaataaaaac
    10141 tcttattagt cgtattaaag atactaataa gagttttttt tgtaataaat ttaaagtcaa
    10201 aaaagtgata tactaaaaaa tatataaata ggagataatt aattataggg aaatttcaaa
    10261 ataatttaat tttaaaaata tgaagaggtg caaaaagtga gcgtaaaaga tgaaattaat
    10321 aaacttatag agacaataaa ctaccataat gaaaggtatt ataatcaaga tagcccagaa
    10381 atatcagatt atgaatacga taagttaatg aaggagttaa tttctttaga agaagaacat
    10441 ccagaactaa agagaattga ttctccaaca aatagggtag gaggtaagcc attagataaa
    10501 ttcaatcaaa ttgtacataa aactcctatg ttgagtttat caaatgcttt ttcagagcaa
    10561 gatttgagag attttgataa gagagttcaa gaatatgtgg gagaaaatgt tgaatatgtt
    10621 gttgaattta aaatagatgg tctttctgta ggtctaactt ataacaatgg agagtttgaa
    10681 aaaggagcaa ctagaggaga tggagttgta ggagaagata tttctcaaaa cttaatgaca
    10741 gtaaaaagca tacctcttaa gataaatgat aaaaaagaac taatagttag aggagaagta
    10801 tatatatcaa aagataattt tagaaaagta aatgaacaac aagaagaaca agaacagcca
    10861 ttatttgcta atccaaggaa tcttgcagct ggttcattga gacaattaga cccaaaacta
    10921 acagcaaaga gaccattaga tatatttgta tttaatatgg aaaatataga agaatatgat
    10981 ttagaaagtc acagtgaatc actagagtac ttagaaaaac taggattttc agtaagtcag
    11041 agttataaag tttgtaaaag tatagatgaa gttattgagc atattgaata ctggacagat
    11101 aacaggggta atttagacta cgaaatagat ggtatggtaa taaaagtaaa caatataaaa
    11161 caaagagacg aaatgggata tacagccaaa agtcctagat gggcaattgc atataaattc
    11221 ccagcagaaa agaagaaaac taaaatagta gatataatag tagaagttgg gagaactggt
    11281 acaataacac cttcagcaat tcttgaacca gtcagacttg caggtactac tgtaagtaga
    11341 gctacacttc acaatgaaga ttatataaga gaaaaagata taaaaataaa tgatacagta
    11401 ttagttcaaa aagcagggga cataatacct caagttttag aagttataaa agaggaacgt
    11461 actggagatg aaatagattt taaaatgcca gaaaagtgtc cagtttgttc agagccaaca
    11521 ataagattag aaggagaggc tgctgtaaaa tgtatcaata tgtcttgccc agcccaaata
    11581 agaaggggaa ttatacactt tgtgtcaagg gatgcaatga atatagatgg attgggagaa
    11641 tctataatta cactgctatt aaatgaaaaa attattcaag atgtatctga tttatattat
    11701 ataaaaaagg aagatgtagt cgacctagag agaatgggcg aaaaatctgc tgacaattta
    11761 ataaattcaa ttgaaaaatc aaaagaaaat gatttatgga gacttataaa tggattaggt
    11821 attaaattta taggagttaa agcagctaag atacttgcat ataactttaa ggatttagat
    11881 gaagtaattt ctgcaactag tgagcaacta gaagaattag aggaatttgg aagtataatg
    11941 gcaaacagcg ttgtcgagtt ttttaaggaa gacaaaaata tgaacgttat aaacaagtta
    12001 aaaaatgtag gagtaaatac aaaatcattg gataatacag gtgaaaaagt agaaaaaata
    12061 tttgaaggga tgaaaatcgt attgactggt acacttccta cattaaaaag aaatgatgcg
    12121 aaagatatga tagaaaaaag aggtggaaaa gctacatcta gtgttagtaa atcgacaaca
    12181 tttgttttgg caggtgaaga agctggttct aaacttacta aagcaaatga tttaggaatt
    12241 aaagttatag atgaagagag atttttagaa ttattaaagt tatcatcgaa agatgaagta
    12301 aaagctgtat tagagagtta aaaagtcgct taatttagta gaaaactact ataattaatg
    12361 atagaatagt tcagtaagct aataagatta cgaaaaatga caataattaa tatttgacaa
    12421 aattaaaaat tataaaaata aatttataaa aaataaaaaa tatgagaaag gatataaaaa
    12481 atgaccagaa gttatgatag aagtaacgaa cagataagac ctgtaaagat aactagagat
    12541 tatacaaagt atgcagaagg ttctgttctt atagaaatgg gagagacaaa ggttatatgc
    12601 actgcatcag tggaagaaaa agttcctcct tttttaagaa atagtggtac tggatggata
    12661 aatgctgaat attctatgct accaagagca acacaacaaa gaaagataag agatgcatct
    12721 aggggaaaaa ttgatggaag aacacaagaa atacagaggt taataggaag agcaataagg
    12781 tctgttgtag acttgaataa attaggagaa agaactattt gggtagattg tgatgtgatt
    12841 caggcagatg gtggaactag aactgcatct ataacagggg catttgtagc agttgcagaa
    12901 gcaatatata aattatataa agcaaagaca ataaaagaga tgccaataac aaattttgta
    12961 tctgcaatta gtgttggtat tgtaaatgga atacacatgc tagatttatg ttatgaagaa
    13021 gattctaaag cacaggtaga tatgaatata ataatgactg ataagtgtga gtttgtagaa
    13081 gtacagggga caggagaaga gaaacctttt tcaagagcag atttaaattt attgttagag
    13141 ttaggcgaaa aagggaataa agaattaata aggcttcaaa gagaagcttt aggtgaaata
    13201 gcagatgaga tattgggtat ggattatggt aatgaagtag tgattgctac taataatgct
    13261 cataagctag aagaaatagg tgaaatctta aaagattttg aatataaagt gtactcttta
    13321 aaagatgttg atttagcagg tatagagata gtagaagatg gaaaaacatt tgagcataat
    13381 gcgttgataa aagctagagc aatagcaaaa aagactaaac ttatagcaat atcagatgat
    13441 tctggcttag aagtagatgc tttaggcaaa aaacctggtg tttattcagc tagatatgca
    13501 ggagaacatg caacagatga agaaaataga aaaaaactgt taaaagctat gcaaaatgta
    13561 ccaatgagca aaagaaatgc aagattcgtc tctgcaatag cagtagtttt tcctgatgga
    13621 aaagaatttg tagtaagagg aatttgtgaa ggaatgatag gatttgaaga aaaaggtaaa
    13681 aatggttttg gatatgatag cttatttata gtaaaaggat atgataagac ttttggtgaa
    13741 atacctagtg ttataaaaaa ttcaatcagt catagagcaa acgcactaaa acttatgaaa
    13801 caagagttta taaaaagggt agtgaagtaa tgaaaatagg catagtaagc gacacacata
    13861 tgatgattaa aaatatggaa aaaacaatac catatttgaa agaatgtgac ctaataattc
    13921 atgcaggaga taactttact gattcaagat atattcatag catgactaat gtaggtatta
    13981 ttgcagttaa aggtaattgt gactttgatg ctgttgagga agaagttgtc tttgaagtgg
    14041 caaataaaac catattctta tgccatggag ataagtatgg tgtaaaatat ggaactaata
    14101 tgttagaaaa aaaggcaact gaagttgatg ctgatatagt tatatttggc catacccata
    14161 ccccatttag agaaattaaa gatggtgtac tttacataaa tcctggaagc acttcacttc
    14221 caagaggagt atcgtataaa agttttgtca ttatggatat agaagaagat gacataaaaa
    14281 ttgaagaaat acgcatataa aggtgtattt atctaaaaaa atggcaaaaa acgctcaaaa
    14341 aattaaattt tatcaaataa gtacaacgat attaatggaa cacttgtaaa taaaaaaact
    14401 tatgtgatat aattaagtgg ttgaattata aatagaatga attataatac cgttatcggg
    14461 tattacataa atatcactag gaggattaga aggatgaaag cagaactaat taaaaaagag
    14521 ggtaataaag ttactttaaa aataacagta gataacaata aatttgaagc agcagtaaat
    14581 aaggcttata ataaaagcag aaataaatac aatatacctg gatttagaaa aggtaaagct
    14641 ccaagaattg ttatagagac tcaatatgga aaaggtatat tctacaatga tgctatagaa
    14701 atattattcc cagaagttta tccagaagct atcaaggaat tagacataga cccaatagac
    14761 aatccagatt tagatataga agaaataagt aaagataatg ggttggttat ggttcttaat
    14821 gtagaagtta aaccagagtt tgaattagga aactacaagg gtatagaaat agctaaggtt
    14881 gagaatactg taagtgacga aaatgttgat gctaaactac aagaaatggt tgaaaaaaat
    14941 gccagattag taagtgtaga agacaaagct ttagaagatg gagatacagc tataatagac
    15001 ttcgaaggtt ttgaaaatgg tgtagctttt gatggtggta aaggtgaaaa ttataactta
    15061 gttataggtt caaacacatt tataccagga tttgaagaac aattagtagg taaaaaagct
    15121 ggtgaagaag tagaagttaa tgttactttc ccagaagaat atcactcaca agatttagct
    15181 ggaaaaccag ttgtatttaa tgtaaaaata aatgatgtaa aagtaaaaga attatctgca
    15241 cttgatgatg aatttgctaa agatactagt gaatttgatt ctttagatga attaaaagca
    15301 gatgtaagag ctaagttaga agaagaagct aaaaatagag cagatgcaga aactagaaat
    15361 tcagtagtag aaaaagttgc tgaaaataca gaaatagaaa taccagaagt tatgatacaa
    15421 catcaaatag ataatatgtt aaatgaactt aactaccaat tacaatatca aggatttgga
    15481 ttacaacaat tactagagat gactggtaga actatggaag aattaagaga agagagaaaa
    15541 gaagatgcta aaaaattagt taagtcttct ttagtattag aagcaataac taaagctgaa
    15601 ggaattgaag caacagaaga agaatttaag gctgagttag aaaaaatggc ttctgcttac
    15661 aatatggaag tagaaaaaat agaagcttct ttaagagatg ctgacaaaga agatataaaa
    15721 ggtcaaataa aaataagaaa aacaatagat ttattagttg ataatgcaac aatagcttaa
    15781 taaaataaac gctaactaat atagttgacg ttatgaaatg tttagtatat aatgatgtgc
    15841 aaatataatt tagttaaatg cgtataataa tttacagtag cttaaaaaag ctactgtaaa
    15901 ttatatagaa taaagggggt atgaaaccat ggcattagta cctgtagttg ttgaacaaac
    15961 aggaagagga gaaagatctt atgatatttt ttctagacta ttaaaagaca gaataatctt
    16021 cttaggagat caagttaacg atgccacagc agggcttata gtagcacagc tattattttt
    16081 agaagctgaa gacccagaca aagatataca tttatatata aattctccag gaggaagtat
    16141 aacttctgga atggctatat atgatactat gcaatacatc aaaccagatg taagtactat
    16201 atgtataggt atggctgctt ctatgggagc attcttgttg gcagcaggag caaaaggaaa
    16261 aagactagca ctaccaaata gtgaaataat gatacatcaa ccattaggtg gagctcaagg
    16321 tcaagcaaca gatatagaaa ttcatgcaaa gagaatttta aaaataaaag aaactttaaa
    16381 tgagatatta tctgaaagaa ctggtcagcc attagaaaaa attaaaatgg atactgaacg
    16441 tgataacttc atgagtgcac tagaagccaa agaatatggt ttaatagatg aagtatttac
    16501 aaagagaccg tagatagaga gaggtgttaa tatgtctaaa tatgaagaaa aacgacaatt
    16561 aaagtgttca ttctgcggta aaaatcaaga tcaagtaaga agattaattg caggtccaaa
    16621 tgtatatatt tgtgacgagt gtgttgaact ttgtgacgaa attattcagg aagaaataga
    16681 agatactata gatgaagata ctacatcttt accaaaacca aaggaaatga tggagattct
    16741 aaatgattat gtaataggtc aagaaaaagc taaaaaagct ttatcagttg cggtttataa
    16801 tcattataag agaatataca gtaaaaaatc tagtagtaag gatatagaaa ttcaaaagag
    16861 taatatactt ttattaggac ctacaggttc tggaaagaca ttacttgctc aaactctagc
    16921 aagaactcta aatgtacctt ttgcaatggc agatgctaca tcattgactg aagctggata
    16981 tgttggagaa gacgttgaaa atatactttt aaaacttatt caagctgctg attttgatat
    17041 agaaaaagct gaaagaggta taatatatat agatgagata gataagattg caagaaaatc
    17101 tgaaaaccca tctataacta gagatgttag tggtgaaggt gttcaacaag cacttctaaa
    17161 aatattagaa ggtactgttg caaatgttcc accacaagga ggaagaaaac atccccatca
    17221 agaattttta aagatagata ctactaatgt attatttata ttaggtggag cttttgatgg
    17281 attagagaag ataattcaaa aacgtggtgg agataaaaca ctaggttttg gagctaaaat
    17341 agaaagtaaa aaagaattag atttagggaa gctgtatgaa aaagtacttc cagaggattt
    17401 attaaaatat ggtataatac cagagtttat aggaagaata ccagttttag ctactttaga
    17461 gttgttggat gaagatgcat taatgcagat attacaggaa cctaaaaatg ctctagtaaa
    17521 acagtataaa aagttattgg agttagatga tgttgagcta gaatttgaag aaggtgctct
    17581 tcgtgcaatc gctaaaaagg ctatagaaag aaatacagga gctagaggac ttagaagtat
    17641 agttgaaagc gtaatgatgg aaactatgtt tgaagtacca tctagagata atataaagaa
    17701 ggttatagta acagagaaat ctgtgaacga agactcagta aatcctatca tagtacttaa
    17761 ggatcaagag gaaagtgcat aagatgatga aaaggagctt taaaagctcc tttttttatg
    17821 ttaatatgct aaatataaaa taggtatcat tatcataaaa atgtgagtaa tatacctaga
    17881 ataccaaaga cgaatacaat tgcaatggaa ccaatttttc ctttatataa aagataaaat
    17941 gatgctataa atgcaattat tccatataaa tcccaattaa cttctttggc agatgaaaaa
    18001 gctgctgata atatgaatcc tagagtaatt ggtctaagaa ctgtcaataa gttttctacc
    18061 tttttattat ttttaaattt agaaaataac tttgatataa tagttagacc tattaatgca
    18121 aaaattgtaa caccaatagt tgcagctaga gaaccaaaga agccagcagt cttaaatcct
    18181 acaaatgtag cactatttat tgctacagga cctggtgtag attgtgatat agcaagtaaa
    18241 tccaaaaact cttgattagt tataaagtga tatttagata taaattcttg ctggataaat
    18301 ggtagcatgg cataaccgcc accaaaacta aatgccccta ttttaaggaa tattaagaag
    18361 agtagtatta tttctgacat agactatata cacctcctcc aattattaaa taaatagggc
    18421 ttatattgaa tacaccaaca gataaaactg ctataattaa tagtagtatg ttagttttag
    18481 actttggtag tttcttgaac atgctcgtaa aagaatacaa tataagagct attacaacag
    18541 gcgcaactcc tttaaaaaat ccatctaaaa ttttagatga tgaattttta aagtatagat
    18601 acgataagac taagactata caaaatgatg gtaaagcaga gcctaaggta catataagaa
    18661 ctcctggtat accataaagt ttatatccta ataatacaga tatattaact gccagtactc
    18721 caggataact ttgagctatg gataaataat caataaattc ttcctgctct aaaagttttt
    18781 gtttgtttac aagttcatct tgtataagag gaaccatagc gtatccacca ccaaatgtaa
    18841 acgcaccaat ttttaaaaag gttaaaaaca atttcaacat aatagaaacc tccaaaagat
    18901 aaataacatc taagtatact aatattaata ttatatcata ataaaaagat aatatagtat
    18961 gatagtattc taagaattta tcattaatgt tgaaaattaa cttgttcaaa tatataatat
    19021 taaaagcaaa gcaattagac tagaaaagga gtaatgttga tggaacaaaa ttatactaaa
    19081 atagatcatg aattaccatt aattcctcta agaggattag ctatatttcc atatatgata
    19141 ttaaattttg atataggtag agagatatct cttaaagctt tagaccaagc tatgatggat
    19201 gaagaactta tatttttaac ttctcagaaa gaagcagagg tagatgagcc aggagaagag
    19261 gatttttatc atgtgggaac aatctgtaaa gttaagcaaa tgataaaatt accaggagat
    19321 acagttcgtg ttttggttga aggtgtatca aggggtagag taaagaaaat agaacaagaa
    19381 gatgggtatt tcagagcagt aatagaagaa atagtattta atagtgataa tttggatagt
    19441 gaaacagaag ttgaaattga agcatttgta agaaatgtat ttgatgcctt tgaagagtat
    19501 ataaatattg gaaatagggt atcaccagaa atattaatat ctctagctga tatagaagat
    19561 gtagatagat ttatagatac gatagctgca aacatatatc taaaatcaag ccaaaaacaa
    19621 gaaatattag aagaatttga tattagaaaa agacttgaac ttatttattc tatattatta
    19681 gaggaaatag atatattaaa aatagaaaag aaaataactt taagagtaaa aaaacagatg
    19741 aataaggttc aaaaagaata ttatcttaga gaacaattaa aagctataca gaaagaatta
    19801 ggtgaagaag aagatataaa ttctgaagca gatgagtata gagaaaagct taaaaaaata
    19861 aaagcaccta agacaaccaa agaaaaaata gaaaaagaga tagataaatt ttcaaaaata
    19921 tcttctatgt ctccagatgt atctgtaagt agaaactatt tagatactat attctcattg
    19981 ccatggaata aagagacaaa agataaatta gatataacta aggcaaaaga tatattagat
    20041 gaagaccatt atggattaga aaaagttaag gagagaatac tagagtatct agctataaga
    20101 actcttgcta agtcacttaa agggcctata atatgtctag ttggtcctcc aggaacagga
    20161 aagacatcta tagtaaaatc aatcgccaga gcattaaata gaaaatttgt aagaatatct
    20221 ttgggtggag ttagagatga agcagaaata agaggtcata gaagaactta tgtaggttca
    20281 attccaggaa gaataattaa tggggtaaaa gaagcccaaa caaaaaatcc tgtattctta
    20341 tttgatgaga tagataagat ggcagcagat tataaaggtg acccagcttc agcaatgtta
    20401 gaagtattag acccagagca aaataaggac tttgtggacc attacttaga aatacctttt
    20461 gatttatcaa aaatactatt tgttacaaca gcaaatagtc taggtaatat accaagacca
    20521 ttattagata gaatggaagt aatagaagta tctgggtata tagaagaaga aaaattaaat
    20581 atagcaagaa aatacctact tccaaaacag attaaagaac atgctttaaa agaaaacttt
    20641 ataaaaatag atgatgaaac tttaagaagt ataattaacc attatacaag agaagctggt
    20701 gtaagaacac tagaaagaac tattggtaag atttgtagaa aggttgccaa aaaatacgtt
    20761 gaagacccaa ctcttgaaga ggttgttata aataagtctg acctagagac atatcttgga
    20821 aaagacatgt ttaagtatca acttgcagaa gttaatcctc aaataggatt agtaaatggt
    20881 cttgcatgga cagcagttgg tggagttaca cttgaagtag aagtaaacgt acttaaaggt
    20941 aaaggagaaa ttgtattaac tggaaaatta ggagatgtta tgaaggaatc tgcgaagaca
    21001 ggtatctctt atataaggtc aatagtggac aagtttgata tagaccctga attttataag
    21061 acgaatgata ttcatataca cattccagaa ggcgctgttc caaaagatgg accatcagct
    21121 ggtataacta tggctcttgc agtaatctca gcacttacta aaagacctgt tcctggaaat
    21181 atagcaatga caggagagat aactttaaga ggtagagtct tagcagtagg aggggttaaa
    21241 gaaaagttat tagcagctca tagagctggt ataacaaaag tattaattcc taaagaatgt
    21301 gaagctgatt tagatgaaat accagaaaat gtaaaagaaa aaatggagtt tgtgcttgta
    21361 gaacatatgg atgaagtatt agaacaagct ttattaaaga gtggtgaaaa taatgaaaat
    21421 tagaagttca gagataacta tgagtgctgt gaataaatca cagtatccag cagaaggaat
    21481 cccagagata gcattggctg gtaggtcaaa tgttggaaaa tcatcaataa taaatacatt
    21541 gcttaacaga agaaacttcg ctagaaccag tcaaacacca ggaaaaacta gaactataaa
    21601 tttttatcta ataaataatg aattttattt tgtagacctt ccaggctatg gatatgcaaa
    21661 gatagctaag tctgaaaaag aaaagtgggg aggtataatg gaaagatatc tagaaagtag
    21721 acaagagtta tgttctatat tcctacttgt agatattaga catgagccta cagcagatga
    21781 taagcttatg tatgaatgga taaagcactt tggatataac tgtgtagtta ttgcaactaa
    21841 ggcagataaa atatctagag gacaatatca aaaacacata agtataataa gaaaaaaatt
    21901 gcaaatggaa agtagtgaaa aagtaattcc tgtttcttca ctaaaaaaga ctggtgttga
    21961 agaattatgg gaagagattg taaatcaata caatcaacat ggatatgaaa taacagtaga
    22021 ttaaatttaa aatgttaaat attgacataa gatttatttg aataataaat gtaataatag
    22081 ttagttttaa ttatattaat attaaagcaa aatataggat gccttaaaat agttttaatg
    22141 aacttttttt ggcatcctta ttgtttataa acaataaata tcagtaattt tgctaaatac
    22201 taataaattt tagtaaggta tgggaaaatt agtataaatt agttcaacta aaaaattatt
    22261 gacttatgta gtcagcaata aatagcattt aagaaataga tttatctgtt tttctcataa
    22321 agaaaacagt tagtataagc cctataaaac atactgatgc tgcaatccaa taagactgat
    22381 tgctagctat aacataggct gatgtgtgac ttttaaactt ttgattgtat gtaagacttt
    22441 gtgcaaaaaa actactaaaa aatgctgttc caagagcaaa tgataaatta cgaatagttg
    22501 caagaaaact gcctccgtac ccctgttttt gaggaggaac atctgctaaa atactactat
    22561 tgtttggtga actaaatgct cctagcccaa gtccaaaaat aactaaacat acaattaaaa
    22621 gatacaatgg agaatctgct tttaaaagtg ccattccaac tagtgagatg gtcataaatg
    22681 aaaaagatgc tgttagaatg ttttttgcac cgtgtttatc agaaagtgaa cctgcaatag
    22741 gtgctataag taccataata agtggatgta cagtcattac ataaccagcc atcataggag
    22801 ataaattttt taattgttct aaataaaatg gaagtagaaa agatactgcc atttgaggaa
    22861 aataacaagc tactccaatg atattgccta cagtaaagtt tctattttta aatagaggta
    22921 ggtcaataaa gctttgttca aattttactt ctcttaatgc caacaataag aagattataa
    22981 gtgctgttat acctaaatac aatcttgtat tacccttctc attcattgca agtaatataa
    23041 gcgtagtgca agatgcgaaa agtagaagac ctgaaatatc aaagctcttg ttttgttcat
    23101 ccaaaacagg tttaggcaga aatttaatgc caagaaagac tgctataata ccaataggta
    23161 tattgataat aaaaatagaa ggccatccaa aatgttctaa aattatacct ccaattacag
    23221 gaccactcat atttcctata ccaactacag ctccagtgat tcctatagct tttccttttt
    23281 catttgctgg aaatgcattg gaaactatac caagcccagt agccattaaa atacttgctc
    23341 caactgcctg aaagattctt gaagaaatta atgttgataa attgtttgac atactacaca
    23401 gaaaagaacc tattgtaaat ataaaaaatc ctaatgcgta tagacgattt gaacctactt
    23461 tatcaccaag ttttccgaaa aataacatag tagctgttac aacaagcata taagctgtta
    23521 caacccactg aatttgtgac atgttaattt taaagtcagc agcaattgta ggatttgcga
    23581 tatttagtat acttccatca agagtagaca taaatacacc aatacagatg ataatcaata
    23641 ctatccattt ttgtttcatg ataacactcc aatcttaata gattttataa gtacgatata
    23701 attgtaaaat aaacaacaaa aaaagcacct gtatattagg taccttgaat gtgtctaatt
    23761 tcatgaataa atacggtagt tgaaggattt gtatagatta ctcatgtaaa attacataaa
    23821 ggtactcata tactattttc ttctaccatc cagattttac tgtcggcttt ggaatatgac
    23881 caaattatgc cataaggctc gcgggctttt accgccgata gggaatttca cccgtttatg
    23941 aagaaaaaca gatgtatttt gtaatattat tttaacataa aattataatg gaaacaattg
    24001 gatttaagta atatccagct gtattacaat acaaatattt caatatggac acttaaattg
    24061 tatttataga tagaataagg tacagtatga gaatctataa taattagata aaattaatat
    24121 gttattagta ttagatggta agttttaaat ttgtcaaaat cagaaagctg taataaaatt
    24181 cttataatat gaatatctta attaagagat attgtaaatt atgaggaggc gctaatatgg
    24241 aaggaaaggt aaagaaggca ttcccaggtg gaaatacatc acaaggctca tattcttttt
    24301 ttgactatat aattcctgaa aatgtaaaaa gagttttttg tttaaagggt ggacctggag
    24361 taggaaaatc ctcacttatg aaaaaaattt ataaagagtt tttgcataga ggatatgata
    24421 ttgatttata tcattgtcct tctgacccaa gttcgttgga tggtttagtt ataaagaaat
    24481 taggcgtggt acttatggat gcaactgccc cacatactat ggacccaaag ctacctggag
    24541 ctttggatga gataattaat ttaggtgatt actggaatac agaagaatta gaaaagaata
    24601 aacaagaaat atcagtttat gataaagata ttagtaattc atttaaaaga gcatttaagt
    24661 ttttaaagtc agcagaacca atttttagag acatagaaga aaaatattct gattgtatgg
    24721 attatggcaa agtaaatttg gtcacagaag aatttataaa aaggttattt gatggagtta
    24781 aaagcacagg aaatctaaaa agagaaaagc atttatttgg ttcagctata actcctgtag
    24841 ggtgtttaga ttatacagaa aacattttaa aagatgtaaa gaaagtctat tatttagatg
    24901 gagaaatagg aactgggaaa actacattat taaacaaagt atataagaaa gctactgaaa
    24961 aaggtttaaa tgtagaagtt tatcattatc cacttattcc agagaaaatt gaaactgtac
    25021 tattaactga tttagatata ggcataacta taagtacgac ttttaaagaa gaagatacaa
    25081 ttgatttaaa tcagttttta aatagagaaa aactacttaa atatgaagaa gatattaaat
    25141 ttgatgaaaa agttttagat gatttaattt gttgggcagt actaaatctt aagagagcca
    25201 agtctaaaca tgatattata gaaagttact atagtcctaa tatgagattt gatgaagtag
    25261 ggaaattaag agataagtta attaaaagaa ttttaaaata tgaggaaaac ttataaacgt
    25321 atatgtttaa aattaatgtt aataataata taatttttag taaattggtg tagctttgag
    25381 gctatgccat tttacttttt tttgctaaaa aatgatatta ataatctcca aagccattat
    25441 ttgtttttca taattattat ttcctttcct aatttatgtt tttttataat atcacaacaa
    25501 tgtttattga aagtttatat aattttaaga acatctaaaa atgcatactt gatattggta
    25561 aattagtgat aggagttcaa tgtatgatat tatatactaa agatgaattt taatatataa
    25621 ctttgatata ataagcatat gtaaatgttt aatatttaat taaagaaaat tatcatataa
    25681 aaataaattt gtaaggagca atacagtatg aaaaaacaat atgtgtttat aatactagca
    25741 agtactattt taggtatact tgttggaacc atatttagag attatagcaa tgaagaaaca
    25801 gtttatacaa gcaaagacaa gtcaataaga aaagaaataa aaatgacaag aaaatctata
    25861 aaagaattaa ccaaagagaa agacaagtta gataaagaaa ttaattcaat gaaacaaaat
    25921 caaaaaaatt ctgaagaaat atcagaaata gaaacactta gagatagtct atcatattca
    25981 gatgtaaagg gtagtgggat aatattaagt atagatgcta tagatgaaaa aacagggaat
    26041 atagctaata gtatagacta taataagatt cttgtaaaag ttataaatga attaaaagta
    26101 aacggaggaa aatacataga aataaatggt caaagaatta atcaatattc agaagttgta
    26161 cttgcaggaa gccatataaa tataaatgga gttcctgtag cacaaccata tgaaataaag
    26221 tgcattggag ataaggagaa attatctaat tatgtagata aaggtagtaa atatttagat
    26281 agcatacaaa ccaattatcc aatgaaaata agcataaaaa ctgaagaaaa tatctctatg
    26341 aaaaaaatga atatacctaa taaattaaag tatataaagg gtgattaata ttatattaga
    26401 ggaaagcagt ttgccaatgt taataccaga aaaacttgca aagaaatata atgtatttcc
    26461 agttgagttt caagatgata atttagttgt agaaatagaa gatgaggata tttatgctct
    26521 acaggacttg agattagcta caggtaaaga aattatattg aagaaggaga aaaaagaact
    26581 tataagtgaa aagattgaaa aatattataa tagctttgaa cttgatgagg attatgcaaa
    26641 aaaattattt gaaaatcttt tagaaaaagc tataaaagaa aatgcaagtg atatacatat
    26701 agaaccattt aaagaacatt taatagtgag aatgagggta gatggagaac taaaggaagt
    26761 atcaaagttt ttagttgatg tctatccatc attgtctacg gttataaaat taaaggcatc
    26821 aatggatata acagaaaaaa ggcttcctca ggatggtagg gtggatataa agttaggtgg
    26881 aaatttaatt gacattagag tttcatcaat accaactgtc tatggtgaaa aaatagtttt
    26941 gaggatactt aatagagata cttttttaaa agacaaatta gaacttggtt tttcagagga
    27001 agctataaaa tctataaatc gtataataaa taaaagagca ggagtattat tagtgacagg
    27061 tcctacaggt agtggaaaga ctacaacagt atattcctta ttaaatgatt taaaaggaat
    27121 aaataaaaat attatgacta tagaaaatcc tgtagagtat aagatggatg gcataagtca
    27181 gattcaagta aattcaaaag tagggttgag ctttgatgta ggtcttagag cgatattaag
    27241 acaagaccca gatataataa tggtcggaga aataagagat gcagaaacag ctaagatagc
    27301 agtaagagca gctataacag gtcatttagt tataagtact ttacatacta atgatgcagt
    27361 atcttccata gctagattac tagaaatgca aataccacca tatcttttaa atgcctctct
    27421 tataggtgtt atatctcaaa agttggttag aaaagtttgt aatcattgta gccatgagat
    27481 tatgataaaa gataatttta gtggagatgt aaatactaag gtagctgtag ggtgtgaaaa
    27541 atgtaatgat aaaggatact ttggaagaac agcaatttat gaaatcttag aaattaatga
    27601 tgatataaag acatgtataa gaaatatgga ggattctagt actattaaag atgtggcaca
    27661 aaataatgga atgataacat ttgaagatag ctgtaaaaga ctaataaatg aaaaaataac
    27721 gacattagaa gagtgtttag tagtaaatga aatgatttaa ctgaattgta tagttaaact
    27781 tcgtttggtc agttgtcttt atttaatcta ttttaattaa gatgtttgag tataaattat
    27841 atttaagtga aataaaatta aatgcattaa attagaaaat attaagttta tttaattgta
    27901 attaatataa tttggaaggt attatagtac atgagattat ctaaaaatat attttataat
    27961 ttagaaaagt tttatttagt attaaataga atcagtgata aagaaattag agtaatttgc
    28021 aaagagatag gcatattgct ggaatctgga tgtgaaatta caaaaatatt tgaaatcatt
    28081 gagagtcagt caagtaaaaa agtaaagaat ttgcttagta tagtatcaaa tcatattcaa
    28141 aaaggtaact caatagcaga atcatttcaa ataacaggta tattttctaa attctttata
    28201 agtatgataa aagcaggaga aactagtgga aacttagata taataatgag tgatttatct
    28261 aattattatg ataaagaata caaattaaaa atgaaaataa taactatttc aatatatcca
    28321 ataatactta taatattatc tatcttatca atgttgttta tattcgtatt tgtaatacct
    28381 aattttcagg tggtttttac caacaatgga attgaaccac ctttaataac tagagtttta
    28441 atgggaatat ctacagtggt tacaaataac ttagcttata taatatttag ttttatattg
    28501 ttttcaatag gaagcattta tttctttata acaaatgata atataaaaaa attgattaat
    28561 tcgttaaaat tcaaaatacc ttttataaaa aaaattaatc agttagtagc tacaacaaga
    28621 ttttcaaggg cattgtccgt tttaattagc agtggaatac aaattgtgga agctatagac
    28681 atgtcagcta gtgttataga caacgaattt atttatgaaa ggttaaagat ttctaataat
    28741 tatataaaaa aaggaaatag cataggtaaa tctttgaatt tagcaaatgt ttttccaaaa
    28801 ttatttattt caatggcaaa tacaggagaa gaaagtggga aactagataa aagtctaaat
    28861 gtaataaata aattttatga aaatgaatta aataacaaaa tagagcaaat aatgaaattt
    28921 atagaaccta tgctaattgt atttatggga ttaattattg gcacttttat tttagcaatg
    28981 gttgttccta tgtttgatgc aattactaat ttttagagag aaaaataaat tttattagat
    29041 gaaacttttg aaaaggagag aatatttaat tgataaattt gataaataaa aaacgaaaag
    29101 gatttacact tgttgaaatg attgtagtag taactatttt aggcgttata tctagtatag
    29161 cattagttaa gtatagtaag gttcaagaaa gtgccaaatt aaatgcagac tatacgaatg
    29221 ctgctaatat agtaactgca gctagcatgg caattaatga tgatgaaaag acaatagact
    29281 ctctaagtgt agaaacattg aaggaaaagg gatacctaaa tactgttcca gttcctcaga
    29341 gtacatcagg taaattcgaa cttgtcataa atgatagcgg aacagatata agcgtaaata
    29401 taaattctaa acaattttat ccaaaataat attttatatt gattgctgta agttttaaaa
    29461 ttatttttat ttaagaagga gatggctata attaaaaaaa ttctaaactt agaaataaat
    29521 gatgattata taaggcttgt atttttaaga aaaagtcata aaaaagtttt tatagataaa
    29581 tcaattataa aagagattaa ctttgatata aaaaatgata gtaattcgat tgagaagatt
    29641 aaatatatag aagatgtagt tagatttgta aaagaggaga ttggtaaatc aaaaatatta
    29701 tataaaaatt tatactttaa cattcaaatt caagatatag tcattagaaa tgttgaagtt
    29761 ctaaatacta agaaaaaaag agacatatta agcatgattg aatttgaaat aacacaatac
    29821 attccaataa acataaataa ctattcaata aaatataaag ttataaattc taaaaatgat
    29881 aaattgaata tacaagtaat tcttttccca aagagtatta ttcaattaat taagaacatt
    29941 tcagaaaatc taaatatgaa tccaaagact ataaatataa attttgatat attacaaaag
    30001 cttataaact taaatttaat agagaatttt tcagaagaag gggtatttat agaatgtaaa
    30061 aaaagtcagt taattataaa tataacaaaa gataaaaaaa taagagaaac atacttgcta
    30121 tcaaaagagg gaaaatcata tgagttcata aaaagattat taagggattt taagtatgtt
    30181 tattactatg gtatagaaaa ttgttttatt gaagaatact ttaaatatac agataaatct
    30241 attgaagtaa atccattaag attaaaagat aaagtaaaag tagaaaataa ttttgatgca
    30301 gatttagaag acaataggat aaattatata aatagtatag gaatgattat ttaagtgaaa
    30361 gtggataaaa caataaattt tcttaatgga tttaaaaaaa ctaaaccatg taataaaatc
    30421 tataaaataa taatagtctt aattttaacg tgtattttat ttaaatcatt tatgtatgtg
    30481 aaatctataa atgaacttga agattatata aatcaaaata agctagtgga taaatcaaag
    30541 cacactttac caacaacaga taaaactaga gagaaatttt ctaaaataaa acttaatgaa
    30601 ataaaaaaaa tatatgaaac aattggaata aatagtatta ctaaaatatc gatagataaa
    30661 aataagctag agatggaagg tgtgtgtagt gatttagatg ttttaaacaa cctaaggaaa
    30721 ataaagtaca taaaatatta ttacatagac agtattgtaa gagatggaga atattataga
    30781 tttagaataa aaagttagat aggtgattat ttttgagatt aaataataga gaaaaaaatc
    30841 tacttaaaat attgttaata ttagttatta tattggcaag tgatatagca gtaagttatt
    30901 tggaagaaaa cctaactagt ttgaagaatg aaaagcaaaa tataataaat aaaataaata
    30961 atcaagataa ttatcaaata aaggataata agcatataga tgataaatat atagaaagtg
    31021 ttgaattaaa taaaaatgaa gaattgaaaa ataattttaa taactcattt ttagataaaa
    31081 aaacaaataa agatatagtg ttattagtag aaaatgaact gagagatatt gtgaatgtag
    31141 aatatattaa tcaagaaagt agtttagata atcacaataa aaaacaagtt aatttagaaa
    31201 taaaagttaa tggagaactt gcaaatgtgt tgaaacttga aggtgtaatg aagaagttaa
    31261 gactaaataa taaacttgaa aagatagaaa ttaacaaatt aaaagtcaat aattttgatg
    31321 ttcaaaatat tccaaatgaa tataaacaag aaaaagacaa tattgttgaa tgtaaaatga
    31381 cagttagagt catataacaa aaaagaatag ataaagtaaa aaactattat caattaataa
    31441 aattttatag aggaattagt tttacgataa ttcatttcta caagagggat tgttgatgat
    31501 tttatcgaaa tcctaattaa agttaatata ttgtaccaaa atataagtga atttaataga
    31561 atatttactt atactataga ttgattaata aaaaaatggt ataataacaa attgtacttt
    31621 aatttaataa taagggagtg gaagtcatat tgttagggag aaatgagcta tgcccttgtg
    31681 gaagtggcaa aaaatacaag aaatgttgtt taaataaaga tattgtttca gaaagagcat
    31741 gcagaaaaac tgaactttca caaaaacaat atacagattt atattcaaag ttatatgatt
    31801 attcaagaca ggataagttt aaagaagaat gtgaaaaagc taaagaaatg ttttatataa
    31861 tgcaaaatga tagtgtaaat gaaaaatttg aaagattttt taatacttat ttaatacaag
    31921 accacataat ggaaaataaa aaggttatga cagttggttt tcttgaagaa aatggtgcaa
    31981 acctaagtca atcagaagta tcaatattaa aaagtatgtt tgaatcatac gtaagtattt
    32041 atgaggtaaa agaagtttct gctggaaaaa tagtattaaa ggactgttta agtggaaatg
    32101 aactttgtac agaagatgta aaacttctga gaagttttaa aatcggaagt tgtatgatag
    32161 ctagaatagt tgatattgaa ggaactaaca tattgataga tataactata agtatatcaa
    32221 atgaggttaa agatataatt ttaaaggata ttatgaatct attcaatcaa tatcaagatg
    32281 tatacaaaga tatgaagtcg ttcttaattt atcatacaca tatactatac aagtatatac
    32341 aacagctttt agacccaagt atagctgaat atttaaagag agaaagagat aaaaacgata
    32401 caaaagttaa ttcagaaaat gaattagctg aagaagaatg taaagtttta gatactctta
    32461 aagaaaatgt agaaaaagaa aatatagatg catgtataga gttttggaat gaatataaga
    32521 aaaatcatga aaatataaaa ggagctgaaa atggttgggc agctgctgtt gaatactatg
    32581 ttaaaaaaga agcaggacaa cctataactc aagttcaaat atcaaagaaa tacgatataa
    32641 gctctagtac actaggaaag agatacaaag atttaaaaat atcttaatta attataaaaa
    32701 gctttaagtc atagataaag aatctatagt ataatatatg tataatgttt agaaagaggt
    32761 attaataatg agtcttagtt taggaaatgc aattgctgta tcaacgaact ttataaaatc
    32821 taacccagct gtacctgtgt acaacggaga agaaatatat gcagttattt atcatataga
    32881 aataggatat aataatggtg tatcacctga taccaaacaa tatataataa tagactataa
    32941 agagtttgat ttatctatgg ataaaaaaaa tgaactaata aatgttgcta aagagtactg
    33001 taaatctaaa ggtattgtag aaaacgaaaa ccttgaaata aaccttttag acaatgaaga
    33061 agcagagaac ttcttagaaa aggtgtttaa taacatgaag gttataagag gtcttataac
    33121 taaaaaagga taaataaatg gactatatag gaatagaaaa tataacacct tatgaaaata
    33181 catatgaatt tagtgtatat gaatatgatg atgaaatcac cttaggtagt gaaaagttat
    33241 atgtgtgtga attaagggtt gtattgatta aagttaattc tctgtatgtt gaaagattgc
    33301 ataaatcagt tgaagcaatg gtcttagtaa aaaacttgaa aaaggattta gataaaacac
    33361 ttgttgtaaa caaaataaag aattttgtgc tagatgagat ttgggtagaa aatctagtaa
    33421 aagagaatat agaagttata tttgtagaaa gctaggaata accctagctt ttttatatta
    33481 caatctaaga gaggaaaaca atatgttaag agtatctaat gtaaaacttg gaattgataa
    33541 agatatatct ttattaaaag atgttgtact taagaagcta aaaataaaag aaaaaaactt
    33601 agtcaaatat agtatatata aagaatctat agatgctaga aaaaaaggaa aaatggaatt
    33661 tgtttattct gtagatgtag aagtcaaaga tgaaaataaa atacttaact caaaaataaa
    33721 agatgtaaca aaaataaagg aaatcagata cataaatgta cctgtgggtg ataaaaaatt
    33781 aaagaataat cctttggtaa ttggtagtgg accagctggt ttatttgcag gtctgctttt
    33841 agctcaaatg ggttatgaac caatcatact tgaaagaggt cttgatgtag acagtagaac
    33901 caaagatata agtgattttt ggacaactag aaaattaaaa aataattcaa acgttcaatt
    33961 tggagaaggt ggagcaggaa ctttttcgga tggtaaactt acaactagaa taaaagatat
    34021 acgatgtaga aaagtattag aagagttagt tcagtttgga gcaccagatg aaatattata
    34081 ctcacataag ccacatgttg gaacagatat attaaaaaat gtagttaaaa atataagaaa
    34141 tgaaataata agtttaggtg gtcaagttag atttaattca aaagtaacag atatagttgt
    34201 atcaaatgat tctatacaat ctgtaataat aaatgatact gaaaaaatag atacagatac
    34261 aattatacta gctgttggac atagtgcaag agatacttat gaaatgttgt attcaagagg
    34321 tataaaaata gttcaaaaac cttttgcaat aggagctaga gttgaacatc cacaagattt
    34381 aataaataaa gcacagtaca aggattttta taatcatcct aggttaggag ctgcagatta
    34441 tagacttata gagcatgttt caaatggaag aacagcatat actttttgta tgtgtccagg
    34501 aggtagtgtc atagcatctg catcaaatga ttttgaaata gttacaaatg gtatgagtga
    34561 acatgcaaga gataaaataa atgcaaatag tgcattttta gtaaatgtaa cgccagaaga
    34621 ttttaagagt gataatccac tagcaggtgt tcatttccag cagaagtatg aaaaattagc
    34681 ttttgaatta ggtggaaaaa attacaatgc accagttcag cttgttggag attttataaa
    34741 caataagact tctttgaatt taggaagtgt tgagccatca tatagaccag gatatacact
    34801 tacaaattta agtgaatgtt tgcctagctt cgtaacagag actatgagag aagcgtttat
    34861 atctcttgat aaaaaattga atgggtttgc aatgtatgat gctgttttga cgggagtaga
    34921 aactaggtca tcatcaccaa taagaatagt aagagatgaa aatactttag aatcagtgag
    34981 tattaagaat ctatatccat gtggtgaagg cgctggatat gcaggtggta tagttacagc
    35041 agcagttgat ggtataaaat gtgcagaaaa aataatacaa aaatacacca ataatacaaa
    35101 aatttaacat aaacttaatg attttttaat atttaacatg aatttaacat tatcactcct
    35161 ataatttgat aaaatacaat aggttataaa atttttagat acagtaatct ctttacaagg
    35221 aggaaataat ttgaacatta taataagtct aatgggtgga ttaggtctgt tcttatatgg
    35281 tatgaacctt atgggagaag gtcttcaaaa atctgctggg acaaagctaa aaaagataat
    35341 aaaactactt actagtaacc tatttatggg agttttggta ggtacaggag ttacagcagt
    35401 aatacaaagt tcaagtgcta ctactgtaat ggttgttggt ttcgtaaatg caggaataat
    35461 gactttaaaa caggcaatag gagttataat gggtgctaat atagggacta ctgttacagc
    35521 ccaattggta tctttcgact taactggtat ggccccagta gcattaggtg taggtattat
    35581 actttattta tttggaaata agcctagaat aaaaaatata gccgaaatac ttataggttt
    35641 tggtatatta tttacaggta tggattttat gaagatggca gtggagccac taagagacta
    35701 tcaaggattt actgacctac ttgttacatt tggtagatat ccactacttg gattattatt
    35761 gggatttggg attacagcaa taatacaaag ttcaagtgca tctatgggta tgttggttgc
    35821 actggcagca gaaggtctag tgccattatc agcagcactg ccaattcttt atggccaaaa
    35881 tattggtaca tgtgtaactt cactgttatc aagtattggt gctaacaaaa atgcacgaag
    35941 agcagctatg atgcatctaa tcttcaatgt tttaggaacg gtgatattct taatattctt
    36001 gaataaacca gtagtaagca tggttacaag ttgggatcca agcaatgttg caagacaaat
    36061 agccaataca catacattgt ttaatataat atctgtattg atacttttac catttacaaa
    36121 tcttattata aaactagcta taaagcttgt tcctgataga gcaggtgata tagatgaaga
    36181 tgaaactaaa actattaaat atatagatga tagaatgata gaaactcctt ctatagctct
    36241 tgcaaatacg ataaaagaag cacttcgtat gggtgagaag gcgaaggaaa gcctaaatgc
    36301 atctatggaa gccttagtag aacattcaac tgaaaaaata gctaagacat atagacgaga
    36361 gagattaatc aatgatttac agaaagctat tttaaattac ttacttaaac tttcaaaagc
    36421 acctttagat gatgattcaa gagaagttgt ggatactttg tttaatacag tcaatgatat
    36481 agaaagaatt ggtgaccacg ctaaaaatat agctgaatta tcgcaagttg caatagattc
    36541 aaatatttct ttctcagaag aaggacaatc tgaacttgat gttatgtata atagagttgt
    36601 ttcagcatat acttatgcat tagaatcaat gagaactgat aatgttgatt tagcttgtaa
    36661 ggttataaaa attgaagaac aagttgatat aatggagaaa tcttgtagag ctaatcacat
    36721 gtatagattg aacaataatc tatgtagtat agaaaatgga gttatttatt tagacgtaat
    36781 aagtaattta gagagaatat ctgaccatgc tgtcaatata gcacaacaag ttatagcgaa
    36841 gagattagga aatgattaaa aataattaat ttaaaataat ataaaattta aaatagagga
    36901 taaataaaag agaaacttta aatgatacgt ttagagtttc tttttttgta aatttatttg
    36961 actaaaattt ttaaatttat acatagttat agtatttaga agaatcgatt tcctgataat
    37021 aaatgatgag atatgtatga ttttgtggca aacttttaaa aaaaacacct atattttgtg
    37081 ttataatctc tttaaaacga atatgctgaa aagagggatt taaatgggaa aaataaattt
    37141 tgattatagt aaagctacag agtttttttg tcaaaacgaa atagatgtca tgcaaccata
    37201 tgtagatgtt gcgcacgaca tgttacataa taaaactggt ttaggaaata cttttttagg
    37261 atggatagac cttcctaaaa attatgataa agaagagttt gacagaataa aaaaatctgc
    37321 tgagaagata aaatcagact cagatgttct tctggtaata ggtataggag gctcttattt
    37381 aggttcgaga gctgctatag atatggtaag tcattctttt aggaatggac ttaaaaaaga
    37441 gcaaagaaag gctccagaag tatattttgt agggcataat ataagctcaa catatattat
    37501 ggatttatta gatataattg aaggaaagga tatttctgta aatgtaatat ctaaatcagg
    37561 aactactaca gaaccagctc tggcttttag aatatttaaa gattacttag agaaaaagta
    37621 tggtaaggaa gaagcaagaa agagaattta tgcaacaaca gatgcaagta aaggtgcact
    37681 tagacagtta gctacagaag aaggatatga gacttttgta ataccagatg atgtaggtgg
    37741 tcgtttttct gtattgacag cagttgggtt acttcctatt gctgctgctg gacttgatat
    37801 tgatgctatg atgaaaggcg caaatgatgc tagagaagca tttcaaaatc ctgacttaaa
    37861 aagtaatgat agttatagat atgctgttgc tagaacaatt ttacatagaa aaggaaaaga
    37921 tgtagagtta ttagtgaact atgaacctca actacattat gtaagtgagt ggtggaaaca
    37981 gctttatgga gaaagtgaag gaaaagaaaa taaaggttta ttccctgctt ctgtagattt
    38041 ctcaacagat ttacattcta tgggtcaata catacaagat ggaaaaaggc ttttatttga
    38101 gacagtactt aatgttgaaa actgtaagag aaatataact ataagttcag aggaagtaga
    38161 cttagatggt ttaaattatt tagcaggtaa gactgtagat tttgtaaatc ataaagcttt
    38221 tgaaggaact ctattggctc atacagatgg aaaagtacct aatttagtaa taaatatacc
    38281 tcaactagat gagtataatt ttggatattt agtttatttc tttgagaagg cttgtggaat
    38341 aagtggatat ttattgggag ttaatccatt tgaccaacca ggagtagaag cttacaagaa
    38401 aaatatgttt gctttactag gcaaaccagg ttatgaaaaa gaaaaggaag agctagaaaa
    38461 aagacttaaa taattttaaa tagttaaggt ttaattaaga ttaaaataat ataatgaaat
    38521 catcgaaaag gagtttactc aaaatagatg atttcatttt ttttatgaaa aattgggaaa
    38581 aatatatttt gataatttat attatattaa gatagggagt ggtatgagat gtcaagaaga
    38641 aagaaaggga taagtttagt aatattagtt gctataataa gttcaatact tagtagtttt
    38701 ctaacaataa ttttagttaa ggacaattta gtaagcaaat ctacaggaag ttctacgccg
    38761 atagttgtaa atgatgatgg taagagtcaa aatatttatc aagcagttgc agaaaaagct
    38821 acgccatctg ttgtaggtat tacaacaact agtgttgata ctagtaatat gtttgcaata
    38881 ccaactgaaa cacaaggtgt aggaactgga atcatagttg attcaaatgg atatatactt
    38941 actaattctc atgttatatc agatggtcaa gctacaagtg taaatgtatt attcaatgat
    39001 ggttcaacaa cttcaggtaa agtagtatgg tttgaccaac aacttgattt agctatagtt
    39061 aaggttgata aaactggact aacaccagca gaatttgctg atagtgacaa agtaaaagtt
    39121 ggagatatat caatagcaat aggtaatcct cttggattag attttcaaaa aacagttact
    39181 caagggataa taagtggatt agatagaact atacaaacag aaaaaactaa tatgactggt
    39241 ttattacaaa ctgatgcaag tataaatgca ggaaatagtg gaggaccatt gttaaatcaa
    39301 aaaggtcaag taattggtat aaacacagca aaagcttctc aagcagaagg attaggattt
    39361 gcaataccaa tcaatacagc taagagtata gttgaagaag taatcaaaaa tggaaaatat
    39421 gaaaaagtaa ctttaggcat aaaaggtaca gatgtatcaa attatgaagc agcaacaggt
    39481 acaaaattaa gtactgataa aggtgtttat gttgcagaag taatatctgg ttcatcagca
    39541 gaaaaagctg gagtaaaggt aggggatatt ataactaaag taggagatac agatataaca
    39601 ggtatgaatg atttaaataa aaagttgtat actttctcaa aaggtgctag tacaaaaata
    39661 acagtaaata gaggcggaaa agcagtaaca ataaatgtga atttctaaat atgaccagaa
    39721 agaaggagac tacatggtaa agtgtagtct ctatttttat ttaattttta tagtatatac
    39781 atctaaatta attaaaatga tgtattgttt atagtttatt gctatatttt cttataaaaa
    39841 taaatttaat aaatatttaa atttaattta caaaaacaat ataaatatca taataaaata
    39901 aaatttttta atattactaa aaatatatag acaaaataat aacaaatata gtattatgta
    39961 attgtagaaa tgcttttcat aggtatattc tatgaagatg aattgcataa aaatgaaaaa
    40021 atggaatgga tattatttag gggtgaaaaa tcatgaatcc attagttata aacttacaaa
    40081 aatgtagcat acatgatgga ccagggataa gaagtacagt attttttaaa gggtgtccac
    40141 ttgaatgtgt ttggtgtcat aatccagaga gtcaaacata tacaaaacaa gttttgtata
    40201 atgaagaaag atgttcaaaa tgtgaagcat gtataaatat atgccctcat aaggcaattt
    40261 acaaaggtga aacaaaaata tgcttagacc aagataagtg tgagttttgt gaaacttgct
    40321 tagactattg tgtaaacaat gcaagagaga ttgttggtca agagtattct gtaagagact
    40381 tggttaaaga gatagagaag gatagaattt tctatgaaga atcgggtggt ggagtaacac
    40441 tatcaggtgg agaagtaatg gctcaagata tggacttcat ttgtggagta ataaacatgt
    40501 gcaaatcaaa gggaatccat gtagcaattg atacatgtgg ctatgcaaag tcagaaaatt
    40561 atgaaagagt tgctaaatgt gcagatttgt ttttatatga tattaagctt attgatgagg
    40621 ataagcacat aaaatttaca ggaaaatcaa atgacttaat actgaaaaat gttaagattt
    40681 taagtgaatt aggggttaac ataaatataa gaataccatt aattgtagga gtaaatgtag
    40741 atgatgaaaa tttagaggtt aaaaaaatga tagagttttt aaagcctttg aatatacaag
    40801 cagtaagtct tcttccatat cataatattg gtaaacacaa gtatgataaa atttataaaa
    40861 agtatgaagg agaagaacta caaagaccat cagaagaaaa attagaggaa ataaaaagac
    40921 tgtttgaagc aagtaatttc aatactaaga ttggagggta ataaaatggc aagaggaact
    40981 tttgagagaa ctaaaaaatt aagagaagaa agtattaatg cagaaccaca catatctatt
    41041 gaaagagcag tacttatgac agaagcttat aaaaaatatg agggaagtgt agagattcct
    41101 gtacttcgtg ctttatcatt taagcattat attgagaata gaactcttag cataaatgat
    41161 ggagaattaa tagtaggtga aaaaggtgat tctcctaatg gagcaccaac atatccagaa
    41221 atttgttgtc acacaatgga agatttagaa gtaatgcaca atagagatat aataaatttt
    41281 agcgtatcag aagaagctag aaaaatacat aaagaagaaa taattccttt ttggaaaaaa
    41341 agacaaacaa gagataagat aataaatgca atgacacctg agtggttagc agcatatgaa
    41401 gcaggtatgt ttacagagtt tatggaacaa agagccccag gacatacagt ttgtggagat
    41461 acaatatata aaaaaggatt tttagattta aagaaagaca tagaagcaag attaaaagag
    41521 ctagattttc ttaatgattt agatgcatat aataaaaaag cagatttaga agctatggca
    41581 atagcttgtg atgcaatggt tatattaggt aaaagatatg ctgagaaagc aagacaaatg
    41641 gcagaagaag aaacagatga agctaagaaa aaagatttat tactaattgc tgaaacttgt
    41701 gatgttgtac cagctcacaa accagaaact tatcatcaag ctatacaaat gtattggttt
    41761 gttcatatag gggttactac tgaacttaat atatgggatg cttttactcc aggaagactt
    41821 gaccaacatc taaatccttt ctatgaaaga gatgtagaaa atggaatact agatagagat
    41881 agagcacaag aattacttga atgtttatgg gttaaattta acaatcagcc agcaccacca
    41941 aaagttggta taacattaaa agaaagtagc acatatacag attttgcaaa tataaacact
    42001 ggtggaataa atccagatgg tcaagatggt gttaatgaag ttagttatat aatacttgat
    42061 gttatggatg aaatgaaatt gatacaacct agttcaaatg ttcaaataag taaaaagact
    42121 cctcaaaaat tcttaaagag agcttgtgaa atatcaagaa aaggatgggg tcaaccagca
    42181 ttttataata ctgaagcaat agttcaagag cttatggaag ctggaaaaac tatagaagat
    42241 gcaagacttg gtggaaccag tggttgtgtt gaaactggat gttttggtaa agaagcatat
    42301 gttctaactg gatatatgaa cattccaaaa atacttgaat taacattaaa taatggatat
    42361 gacccaatta gtaaaaaaca aataggtatt gaaactggag accctagaaa tttccaatct
    42421 tatgaggaac tatttgaagc atttaaaaag caattacatt atatgattga cataaaaata
    42481 gaaggaaatg ctgtgataga aaatatatgt gcaaaacaca tgccttgtcc acttatgtct
    42541 acaatagttg atgattgtat agaaaaaggc aaggattacc aaagaggtgg agcaagatac
    42601 aacactagat atatacaagg tgttggtata ggaactataa cagatagttt aacagcaata
    42661 aaatataatg tatttgataa gaaaaaattc gatatggata cattgcttaa agcacttgat
    42721 gctaattttg aaggatatga agctatactt aatttagttt caaataagac tcctaaatat
    42781 ggaaatgatg atgattatgc tgatgaaata atgcaagaga tattcaatgc atattacaat
    42841 gaggtaactg gtagactaac tgtttgtggt ggagaataca gagtagatat gttaccaaca
    42901 acttgtcata tatactttgg agaaattatg ggagcaagtc caaatggtag actttgtgca
    42961 aaaccagttt cagagggaat ttcacctgaa aaaggtggag atactaatgg acctacagca
    43021 gttataaaat cttgtgctaa gatggaccac ataaaaactg gtggaacatt attaaatcaa
    43081 agatttgcac catcagtagt tcaaggagaa aaagggttag ataatatggc taatctagta
    43141 agagcatact ttaatatgga tggtcatcat atacaattta atgtatttga taaaaatgta
    43201 ctgttagagg ctcaaaagaa tcctaaggat tataaagact taatagttag agttgcagga
    43261 tatagtgacc atttcaataa tttaagtaga acactacaag atgagataat aggaagaact
    43321 gagcaaacat tctaggtttg cttgtaaata aaacttctta ctatataaaa tcgtaactga
    43381 tataataaag tataaatggt ttgattccat ttttaaaatt ttagattaat tatgagctaa
    43441 ctttctaaat aggaagttag ctcgttaagt ttaatgaaaa aatcgttaaa tttatattta
    43501 gtaaatcata cacatattag taagtaatac atacaaaaga ataatttatt atagttataa
    43561 taaggttata ttttataaaa ataagataat tttaagcagt taaaataaag tcaatttagc
    43621 tactttaaaa taaaactaat agaaaaaagg agatttgata tgaaaacttt aggatttatt
    43681 ggttcaggga atatgggaag tgcaatgata ggtggcatag tacagtcagg attagtaaag
    43741 gcagaagata taactgtatc agatttaagc caagttgcct tagataaagt aaaagaagaa
    43801 tttggaataa atactacaac tgattcaaag gtagctgtta caaattctga tgtggtaata
    43861 gtagcactta aacctaatat atgcgaaaag gctttaactc cacttaaaga attaatagat
    43921 gcaaataaaa taatagtatc tatagcagca ggtaaaacta tagaaagtct ggaaagtttt
    43981 ataggttcag ataaaaaaat agtaagagta atgcctaata ctccagccct tgttggcgag
    44041 ggaatgtcag ctctttgtaa aaatagtaat gtaacagatg aagagttaaa tatgattaag
    44101 gctctgtttg aatcttttgg tgaggcagaa atagtatcag aatatttaat ggatacagtt
    44161 acaggtatta gtggttcttc tcctgcatat gtatttatgt ttatagaagc tatggcagat
    44221 gcagcagtat tagctggaat gccaagacaa caagcttata aatttgcatc tcaagcagta
    44281 atggggtcag ctaagatggt tcttgaaact ggaaaacatc caggtgaatt aaaagatatg
    44341 gtttgttctc cagcaggaac tactatagaa gctgttaagg tattagaaga agaaggcttt
    44401 agagcagcag ttataaaatc tattgttgct tgtatagata agtctaaaga tatgagcaaa
    44461 taacagatgt aattaagttt actatatggt ttcttgaatt gtttttttag tacttattta
    44521 tatctataca taaatgtatt tggtataagt aaaataagag taatttacta taaaaaggaa
    44581 tttaatgatt gagtgagaat ttattactt
//
LOCUS       DAELSY010000014        44216 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.014, whole genome shotgun sequence.
ACCESSION   DAELSY010000014 DAELSY010000000
VERSION     DAELSY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44216
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.014"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            402..1235
                     /locus_tag="KPG09_000680"
     CDS             402..1235
                     /locus_tag="KPG09_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG0965307.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1328..2260
                     /locus_tag="KPG09_000681"
     CDS             1328..2260
                     /locus_tag="KPG09_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0965308.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKEYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2312..2794
                     /locus_tag="KPG09_000682"
     CDS             2312..2794
                     /locus_tag="KPG09_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0965309.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2806..3675
                     /locus_tag="KPG09_000683"
     CDS             2806..3675
                     /locus_tag="KPG09_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG0965310.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGVIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKAHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3718..4473
                     /locus_tag="KPG09_000684"
     CDS             3718..4473
                     /locus_tag="KPG09_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG0965311.1"
                     /translation="MTTYKKLKKSIFKISIFSICLLVSLVDINPIFAGSSSNETSNSD
                     TIKVLNAKSLNFKQGNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEDSSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDFPVKDFESINKYQDFYFNKN
                     GNLVISFEEYEVVPGYMGAVEFVIPNKVIKRLR"
     gene            4515..5336
                     /locus_tag="KPG09_000685"
     CDS             4515..5336
                     /locus_tag="KPG09_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HBG0965312.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANYER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKRETGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5341..6102
                     /locus_tag="KPG09_000686"
     CDS             5341..6102
                     /locus_tag="KPG09_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBG0965313.1"
                     /translation="MNTGSKDIDTLNVNFRGIEIKAVLVYKNRKNISIKIDPFGKIIV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKYKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLKDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERTVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6263..6520
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6633..6872
                     /locus_tag="KPG09_000687"
     CDS             6633..6872
                     /locus_tag="KPG09_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG0965314.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            7142..8137
                     /gene="ilvC"
                     /locus_tag="KPG09_000688"
     CDS             7142..8137
                     /gene="ilvC"
                     /locus_tag="KPG09_000688"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBG0965315.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            8190..9878
                     /gene="ilvB"
                     /locus_tag="KPG09_000689"
     CDS             8190..9878
                     /gene="ilvB"
                     /locus_tag="KPG09_000689"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBG0965316.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKTFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWSAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKAGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSNMEELGKVLDVIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(10236..11366)
                     /locus_tag="KPG09_000690"
     CDS             complement(10236..11366)
                     /locus_tag="KPG09_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG0965317.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLDNKTITVKHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(11445..12152)
                     /locus_tag="KPG09_000691"
     CDS             complement(11445..12152)
                     /locus_tag="KPG09_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBG0965318.1"
                     /translation="MSIKNGKDFLYVIWKDPKSRRQFIIGKLAKNGLYEFSYEYEIDE
                     AIKAGFNALISFDDISKTYYSEDLFSPFASRLPDKKRKDINKILDKYDMSEYDTYELL
                     KRSGARLPIDTLEFVDPIPLDIDKNIIRYFYIAGVRHYAGCEGHECDNSIELESNEAL
                     VLELEPENKFDPHAIKVFDKKENHIGYIPRYYSGELTSIINRGLKYNCIIKFVNKENN
                     CDECIKVKLEILFDEYN"
     gene            complement(12165..13034)
                     /locus_tag="KPG09_000692"
     CDS             complement(12165..13034)
                     /locus_tag="KPG09_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HBG0965319.1"
                     /translation="MIKNFDEWKEYDGFSYGSGRSEKIWLINEKTKDIGLFKFPKTKE
                     TGEYWAEKLACEIASLLKIPCANVEIGTYNKRIGSMSYLINKNDEKLIEGVWLINKYY
                     PCYNTDKLYDYKNNEYYSLKMIFKSIEEYNIKNDFLKMLIFDYLIGNRDRHQNNWALL
                     ESQNKITLSPLYDNGSSLCCYVNIENIDSFFTDHQRFEALINTRSRSRIRIDGKNKKE
                     PTHLEVLKYLNSNYYEQTIDFVEKIINLMNEVNINTLIDNYSENIISPKLKKLLKIYL
                     KEKVLRIKNIYNL"
     gene            complement(13654..14160)
                     /locus_tag="KPG09_000693"
     CDS             complement(13654..14160)
                     /locus_tag="KPG09_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBG0965320.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            complement(14236..14784)
                     /locus_tag="KPG09_000694"
     CDS             complement(14236..14784)
                     /locus_tag="KPG09_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0965321.1"
                     /translation="MLILKKLRTEKGISLDKLSTDLNINKSTLSRIENGLREPKESFI
                     KDCSDYFGVSTDYLIGKINIDDSNKLTKCLNSTFPIRLKELRKKKELTQAELSKLLNC
                     SLSKIAMLETSKREPVKEDLLRISEFFNVSVDYLLGKTSIENYITTDEISKIIKSYES
                     LPKEAQEHINSYIEFLVDRYKK"
     gene            complement(14796..15137)
                     /locus_tag="KPG09_000695"
     CDS             complement(14796..15137)
                     /locus_tag="KPG09_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG0965322.1"
                     /translation="MATFGERFKQLREEKKLTQDELVSKFNKVYFTSFNKSTISQYEN
                     NKRKPEINILENWADFFDVSIDYLLGRTLVRNHIDTVAAHRVNPHKDLPEEAQEQLND
                     YIEFLMNKYKK"
     gene            15314..15517
                     /locus_tag="KPG09_000696"
     CDS             15314..15517
                     /locus_tag="KPG09_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0965323.1"
                     /translation="MKKRSLKAGRIEAGYTQEELAYKIGIAKSTYNLKENGKRNFTEK
                     EMIMISYILNKTMDELFLEKRLT"
     gene            15565..16374
                     /locus_tag="KPG09_000697"
     CDS             15565..16374
                     /locus_tag="KPG09_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBG0965324.1"
                     /translation="MNNLQIFKNKTFGEIRVIELNGEFWFVGKDIAEQLGYKDTSDAL
                     KRHVDDEDKGVGEIPTPGGNQNMKVINESGLYSLILSSKLPSAKLFKRWVTNEILPSI
                     RSTGTYNMIDLQTKLPTTYKEALQHLIEQVEVNEQLQLESKMKDQVIKELKPKADYTD
                     MILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGKGYTHSET
                     IDITRSNGMPDVKMTTKWTQKGRLFLYDLLKTNNILPDIEKGYSYQTSILG"
     gene            16421..16630
                     /locus_tag="KPG09_000698"
     CDS             16421..16630
                     /locus_tag="KPG09_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965325.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMNKSEKRKISEITKERIRK"
     gene            complement(16640..17014)
                     /locus_tag="KPG09_000699"
     CDS             complement(16640..17014)
                     /locus_tag="KPG09_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965326.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            17090..17287
                     /locus_tag="KPG09_000700"
     CDS             17090..17287
                     /locus_tag="KPG09_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0965327.1"
                     /translation="MLIGDNIKQMLRKRDIKPYKLAKELNIDVSGLYKLLRNKSSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            17318..17710
                     /locus_tag="KPG09_000701"
     CDS             17318..17710
                     /locus_tag="KPG09_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0965328.1"
                     /translation="MDISESIAKQFSDSLKSLIEIEISKQETDRVKSQTIEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTAFMRRLVSTGLIKSLVRGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVEWGDEIAES"
     gene            17691..17840
                     /locus_tag="KPG09_000702"
     CDS             17691..17840
                     /locus_tag="KPG09_000702"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965329.1"
                     /translation="MKLLSLDTNNKNVVTLKKDGKVIADIVFKDIKTGKKISVGILNK
                     KVLVK"
     gene            17911..18348
                     /locus_tag="KPG09_000703"
     CDS             17911..18348
                     /locus_tag="KPG09_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965330.1"
                     /translation="MVEVNVNVKVKVEAPEFTNALLVVANALGGLNLGKAMQIEPINI
                     TDMKEEKKVEVKKAEKIKKVEVKEDVKEEIAEAKEEIEKNNENTTSEVKYTKEEVRTK
                     AAQVSKAGKKDKLKELFGEFGASKLSEVKEEDYSAFMNKLESL"
     gene            18360..19526
                     /locus_tag="KPG09_000704"
     CDS             18360..19526
                     /locus_tag="KPG09_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HBG0965331.1"
                     /translation="MPLQHARLSASGAHRWLHCTPSIKLEENYPPLTSIYAEEGTVAH
                     ELAEVKLMLEYEKISKKAYNARIKKIQKSEYYNSEMEDYIQSYVENVVELVNDSKAIC
                     DDVIVMLEERLDFSEWVPEGFGTGDVVVISDGILQVIDLKYGKGLEVSAIENPQLRLY
                     GLGAYNQFEMLYDIDLIKTTIIQPRLDNISSEEIEVTKLLTWADNVKKKAQMAFNGEG
                     EFVSGSHCGFCRAKNDCRKRAEDNLKLARKYDFADTFALNKYEIADILGFAKNIQDWL
                     KDVQSYALEQAEKHGVKYPGYKLVEGRSNRKYVDEQEVAKVLLNSDYDEEKIYKPRTL
                     KGISDMEKAIGKKSFAKLLSDLIIKPVGKATLVVESDKRSEINSIDSAKKDFEI"
     gene            19541..19876
                     /locus_tag="KPG09_000705"
     CDS             19541..19876
                     /locus_tag="KPG09_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965332.1"
                     /translation="MPLDAGIKDKLDESISIILDLKDRKKSIEKDLDYEKDNLLDMLK
                     RLNITEYNSTEENAKVIVMDFKRESLIKDKVMSTFLEVNDNHLDRVYIPEHIKVSPVC
                     FVSVRAKDN"
     gene            19929..20501
                     /locus_tag="KPG09_000706"
     CDS             19929..20501
                     /locus_tag="KPG09_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HBG0965333.1"
                     /translation="MSNQEITKLVENNLNLVHFSINRFFKSYVEQHPYLYEEFYQEGC
                     IGLYKAALNYDSNRGKFSTVAVSYIRIEMFKALRYGEKHYRNSIEGLDVSVFEDSKRP
                     LKDIHGFYEIQFNYDVDYIRKIARKSYLKDIDKIVDWILQDKKISDIGELLNVSSTVI
                     CGRIKLFSKQVRDLDKFGEITSNIKSIYQI"
     gene            20524..21096
                     /locus_tag="KPG09_000707"
     CDS             20524..21096
                     /locus_tag="KPG09_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HBG0965334.1"
                     /translation="MSNSVQSTKVVTGKVRLSYCNIFKSRAMVEGAEPKYSVCILIPK
                     SDKVTLGRIKKAIDAAKEQGKTSKWGGKLPGNLKTPLRDGDAERADEAEEYVGMYFLN
                     ANSTQKPGIVDKDLNEILDNTEVYSGCYGRVSINFFPYNSAGNKGIGCGLQNVQKLAD
                     GEVLGGARASAEADFSDDFEYEDEEEDFLS"
     gene            21096..23054
                     /locus_tag="KPG09_000708"
     CDS             21096..23054
                     /locus_tag="KPG09_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HBG0965335.1"
                     /translation="MRTLSIDIETYSDLDIKKVGVYRYVDSANFEILLFAYAFDNEEV
                     KVIDLVNDEELPKEVIEALNDNKVIKSAFNANFERTAISKFLNINLKPNEWSCTMIKA
                     LTLGLPGSLDSVSKALKFNEDKQKMKEGKALIQYFCKPCKATKVNKGRTRNLPIHDME
                     KWNKFKEYCKQDVVVEREIRNKLSKYKTTEREIKLWYLDQRINDTGIKVDTELIENAI
                     ECDKRYTEKLTKEAIKITGLNNPNSPAQLKKWLSDKVGFEITSLTKESIPEILKQVDD
                     ENVVRILELRKLMSKTSIKKYEAMKLAKGNDNRVRGLLQFYGANRTGRWAGRLVQVQN
                     LPQNHIEDLDLARNLLKEGDFDLIELLYDSVPDVLSQLIRTAFIPSEGHRFIVSDFSA
                     IEARVIAWFAGEKWRLDVFNSHGKIYEASASHMFKVPIESIKKGDPLRQKGKISELAL
                     GYGGSIGALTSMGAIKMGLDEEELQPLVTTWRNANPNITKFWWDVDKAAKKAIKDRTI
                     VKLQHGIKFIYNPGVLFIELPSGRRLSYLRPKIEPHTTFSGDKITYEGMEQTSKQWKR
                     IDTYGPKLVENIVQATARDCLREAMFNVTDAGYSIVMHVHDELVIDVDKKGGSLEEVN
                     SIMGKEISWAKGLPLKADGYECDYYKKD"
     gene            23066..25483
                     /locus_tag="KPG09_000709"
     CDS             23066..25483
                     /locus_tag="KPG09_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965336.1"
                     /translation="MDIKASEIEHINVRHDGQLMLAIGKNKLETHWKNKSILWSELVN
                     RLSKTLRTQETYTEYRKMSKTEKDRVKDVGGFVGGSLKNGRRKAENIANRSIITLDID
                     YANKDIWEDITLLNDYACLMYSTHSHTENNPRYRLVIPLTRPVLPEEYQAISRMIADT
                     IGIDMFDDTTYQPHRLMYFPSTSIDGDYIFKFQDGEFLNPNEILDLYLDWTDVSYWPE
                     SSRERQKFNTQLKKQQDPIEKAGIIGAFCRSYSIKETIENFLNEVYIPGIDETRYTYS
                     EGSTSGGVVIYDDKFSYSHHGTDPASGILCNAFDLVRIHKFGELDEDAKPETPVNRLP
                     SFTRMSEFASSDTKVRKTIGRENLDKAKDDFGDIDFEDDEWLTRLDYDNKGSYKKTTN
                     NILMFIENDPYLKGKIAYNEFSNRAVVLGKLPWRKDDKLNDWNDSDDSGLRHHIETIY
                     NISSPSKVNDALIIAFENNTFHPIKDYLNSLKWDGIKRVDTLLIDYLGAEDNHYTRTI
                     IRKVLVAAVARVFNPGIKFDNMMVLSGPQGMGKSTFIKKLGGDWYSDSLTTVQGKEAY
                     EQLQGVWLLEMGEMMATKKADIEAVKHFLSKSEDIYRVAYGKRTSRFLRQCVVIGTTN
                     DKEFLRDKTGNRRFWPIDTGVKKVNKSIFNGQLDNERNQIWAEAVELCKANEQLYLSD
                     EEKKEAERQQRTHSEENAKSGIIEEYLNKPITENWYDLSISEKREYIHGSDFGDLKEG
                     TILREKTCVMEIWVELFNGEPKQLTPILSREINDILKGLDDWISYNGNLRFGKIYGRQ
                     RAYVRKS"
     gene            25765..26091
                     /locus_tag="KPG09_000710"
     CDS             25765..26091
                     /locus_tag="KPG09_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HBG0965337.1"
                     /translation="MLESKIEKRLKKEIELLGGKAMKFISPGEAGVPDRIVLLPEGHV
                     IFVELKAPGKKLRKLQQYKMRELRELGFKVKCVSTLKEIDDFIKEVKGWNLNHIHTKN
                     TQLEKL"
     gene            26040..27410
                     /locus_tag="KPG09_000711"
     CDS             26040..27410
                     /locus_tag="KPG09_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0965338.1"
                     /translation="MEFKPHPYQEYTIRKTIDNNNIGLLLDMGLGKTVCTLTAISELM
                     YDYFDISKVLVIAPLRVARDTWSSEVKKWEHLKHLKVSKVLGSKLDRVRALSTDSDIY
                     IINREMVPWIVDFYKRKWPFDMVVIDELSSFKSNKAQRFKSLKKVLPLTKRVVGLTGT
                     PTPNSLIDLWAQMYLLDRGERLGKTITGYKERYFEPGQKNYQTGAIYNWQPKNGAENA
                     IHNKIKDICISLKAEDYLNMPRKIDNKIEIHLDSKILKYYKELEKEKILELDRDIITA
                     SSAAVAANKLLQLANGAIYDNDKNVKELHREKLEALKEIIDVSNGKPIIVFYNYKHDY
                     NRLMKEFKSLKPRTIENSKDIYDWNNGRIQLLLCHPASTGHGLNLQSGGSIIVWFGLT
                     WSLELYQQANARLYRQGQRETVIIHHLICKGTIDEQVMEAIENKDKGQSALLEAVKAK
                     LKEYRE"
     gene            27434..27571
                     /locus_tag="KPG09_000712"
     CDS             27434..27571
                     /locus_tag="KPG09_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965339.1"
                     /translation="MELISPGKIVRCAWCGRRFYKLDKSKVEYCSRSCSKRARRSDLS
                     G"
     gene            27564..28043
                     /locus_tag="KPG09_000713"
     CDS             27564..28043
                     /locus_tag="KPG09_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HBG0965340.1"
                     /translation="MDKESIKEIVRELRKEEREDRKKGVLYNTRLLMKHYNDFKRHID
                     SAVSEAKDVDYLEDDLGKLDNEELYILSIKKSKARTIIMIAHIDSAMKTLKLRQERLH
                     SYEKYRALELYYCDEKTYEEIAEILNCGVVTSRRWINEMVKELGVYLFGIDGLKLTV"
     gene            28326..28875
                     /locus_tag="KPG09_000714"
                     /pseudo
     CDS             28326..28875
                     /locus_tag="KPG09_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
     gene            29070..30044
                     /locus_tag="KPG09_000715"
     CDS             29070..30044
                     /locus_tag="KPG09_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965341.1"
                     /translation="MSNFKKRYIDVYRIDIINKINGSVYSDEEILKEVIKTFFKDKEY
                     CKENKNGINITKDKKFWITEVKCSEDDLIIKVKLEYTKYNQNTNIINAHTKEVEGNKK
                     MDQGDSNKQHLFIKFMKENNIAVVLFERVFVGVPMIELSKNLHLYYEDMIENNKFDNN
                     MSKTSINITQIASKDFIEQILDLDGVSKITLNVDREKFGTDEDNLFSNLNNSRRHNDL
                     IYKPTFRTRYLPSEVKRCCEKYIKGEEFRNKKINRIIIEGEKNKRKLKLDTEGIKLCR
                     EIEVSLDIDNHIDSEDIFEKMNNLIDDLIENNSVFFNLMYQEVAMSEA"
     gene            30065..30574
                     /locus_tag="KPG09_000716"
     CDS             30065..30574
                     /locus_tag="KPG09_000716"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965342.1"
                     /translation="MYKKVLNILLNYYKAINSKEVVELVLTPVLICIISYMFFNSSIN
                     KEVVISLNKDVLTLSGLLVAFGVCIITLLFTTYNKSISEAKDIKTERKVNGFNISYFQ
                     FIQLKAYYTVIMEILVVVICFINTIMLTRYYSNIIFYVLIFFTVHIILSLTTLIISMF
                     HLSWKDRGD"
     gene            30644..31336
                     /locus_tag="KPG09_000717"
     CDS             30644..31336
                     /locus_tag="KPG09_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0965343.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWSSE
                     KKKGAPINKRGAPFSNKNSVGYGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHEITENSEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVANLNKDDIGDDEL
                     KISVDYGDRNDS"
     gene            31314..32567
                     /locus_tag="KPG09_000718"
     CDS             31314..32567
                     /locus_tag="KPG09_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBG0965344.1"
                     /translation="MVIEMIVRINFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANAIL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANSRGLEKTLFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            32573..34015
                     /locus_tag="KPG09_000719"
     CDS             32573..34015
                     /locus_tag="KPG09_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0965345.1"
                     /translation="MLNIYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTLADNL
                     DEMQEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKSVQITFNHSMIINESEKIDMASKSIGIISDETIVSNHPWVEDVNDE
                     LERLKKQEETQKEYDDLIPTNNQEGVIDET"
     gene            34005..35540
                     /locus_tag="KPG09_000720"
     CDS             34005..35540
                     /locus_tag="KPG09_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBG0965346.1"
                     /translation="MKHKDYWRKRFEQLEEVQNNKSVKYYLELEKKYKLAMNSIEKDI
                     LSWYNRFAENEGISLLEAKKLLNTRELEEFKWNVEEYIKYGKENAINQKWMKELENAS
                     ARLHITRLEALKLQIQQHVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWATDGLNFSERIWGKHRPTLINELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFSDLDVEKYEIIATLDLRTSNICRE
                     LDGKIFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAARGEDGKTYYVPSSMKYN
                     EWYEKHIEGRLGKEKADTLRKMQLNESKDRKQFEEYKKVLGNEIPSKFDEYQHMKYND
                     TIRYEEAKKLYKDVNWQVKNQRNLTSGSVHSVPFESKPNSVFDNYKDGKLIQRRYYGN
                     TGKPRLDLDLTDHRNPKQHKIVPHKHDWLADENNHDKVKREKDMELTKAHKIANKDIL
                     NGE"
     gene            35542..35790
                     /locus_tag="KPG09_000721"
     CDS             35542..35790
                     /locus_tag="KPG09_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965347.1"
                     /translation="MDNKFKNLQHLIDSIDLGLDIEFDLYNKPYNISIGDDDTRFITL
                     CPNGDTKYYKNGKDMVDNYNIDGKLLKDLWKDIQIVNM"
     gene            35858..36421
                     /locus_tag="KPG09_000722"
     CDS             35858..36421
                     /locus_tag="KPG09_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBG0965348.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTVTQEDYVNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDMEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            36433..37323
                     /locus_tag="KPG09_000723"
     CDS             36433..37323
                     /locus_tag="KPG09_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBG0965349.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATENNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTANVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDGGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEA"
     gene            37342..37575
                     /locus_tag="KPG09_000724"
     CDS             37342..37575
                     /locus_tag="KPG09_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965350.1"
                     /translation="MPQVKKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLSV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            37585..37986
                     /locus_tag="KPG09_000725"
     CDS             37585..37986
                     /locus_tag="KPG09_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965351.1"
                     /translation="MENNIIDEIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNKILTFRCLRW"
     gene            37980..38327
                     /locus_tag="KPG09_000726"
     CDS             37980..38327
                     /locus_tag="KPG09_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965352.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKLAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            38327..38617
                     /locus_tag="KPG09_000727"
     CDS             38327..38617
                     /locus_tag="KPG09_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBG0965353.1"
                     /translation="MARWGSVDFREFKRVCKKMEKFTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLREGWSGVAYARSLPVYKQGNNYIIEVVNPTEYALTQWGI"
     gene            38727..39533
                     /locus_tag="KPG09_000728"
     CDS             38727..39533
                     /locus_tag="KPG09_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965354.1"
                     /translation="MAKFLDITGKKFGRLRVIKFSKEIKSGKRNRKYWLCKCDCGNFK
                     EIRTDSLTSGLVQSCGCLKKEQDKLNLTDKYQFKKKYKVQNKRLYSIWKGIISRCTDK
                     NNKRYNRYGERNIIVCDEWFCYDNFANWALSNGYSEKLTIDRINNEGNYESSNCRWVD
                     IKTQCRNRSTNILVKHEEKEITLIELSEKTGISYSCLRSRYSKGLVGNKLIEKVKIIE
                     ESRAKLSIEDVKEIRKKYSDGYTIKQLSEIYPVTYSSISNIVHRRTWKNI"
     gene            39882..40319
                     /locus_tag="KPG09_000729"
     CDS             39882..40319
                     /locus_tag="KPG09_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965355.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPRSEDKNFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKNNDTNKMDTVELFGGLKRGDNFE"
     gene            40312..40488
                     /locus_tag="KPG09_000730"
     CDS             40312..40488
                     /locus_tag="KPG09_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965356.1"
                     /translation="MSKALSKEDNYKFTKEQIVNSKKYVNRKDLLNAILKENELYSFS
                     EVEDRINKFMKGVS"
     gene            40489..41799
                     /locus_tag="KPG09_000731"
     CDS             40489..41799
                     /locus_tag="KPG09_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBG0965357.1"
                     /translation="MALGGGTFVTQNKILPGAYINFISAKRATSSLSDRGIVAMPLEL
                     DWGIDEEAFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVILLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSIENKIKDKDLVESSLIYWATGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAIKVINAMSKLYMTVSVN"
     gene            41816..42286
                     /locus_tag="KPG09_000732"
     CDS             41816..42286
                     /locus_tag="KPG09_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG0965358.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVESFALLNGML"
     gene            42345..43172
                     /locus_tag="KPG09_000733"
     CDS             42345..43172
                     /locus_tag="KPG09_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965359.1"
                     /translation="MEFESGIKLGKLTLIKYLGRNKHSKKVWLCQCECGNKVERVENN
                     LKQSIKNEKPPHCGCSPNWKGQNKRFKDITGKTFGKLTVLKMSGKDKYSHNLWLCQCE
                     CGNRTITSTSALEQGKAQSCGCIRKEILLERSTTHNKSNDSLYRVYHRMIKRCTNKSN
                     KDYNYYGGRGIEVCNEWLEDFINFYNWSIENGYRKGLTIDRINVNGNYEPSNCRWVTW
                     EVQRNNKRNSIRVNYKGEMITLKQCAENLNVKYLFLYNQLVTKKIPLEEVIKNIGME"
     gene            43244..43684
                     /locus_tag="KPG09_000734"
     CDS             43244..43684
                     /locus_tag="KPG09_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0965360.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            44025..44186
                     /locus_tag="KPG09_000735"
     CDS             44025..44186
                     /locus_tag="KPG09_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965361.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
BASE COUNT        17951 a         4566 c         7961 g        13738 t
ORIGIN      
        1 tcgtgattta taaataagat tagaaaattt tatttgataa caaaaattag acaatatgaa
       61 tatataatgt atgtattaaa aatgtggcaa atagttgttt tggtatttat tatttgttta
      121 atatttctaa tttataaaaa aaaaattata aattttaaaa attataaatg acattttaac
      181 tagaattaat tgtaaaaaac actatataat actcaatttt caacattcta tacaattata
      241 cagaaattat acagcataat aatcaccgaa ataacaaatt ttaaaaatta agcaaaactt
      301 tttgtcgaag ttcaacgtct atatatatgt agtgtaaatt tttgggataa aatactacaa
      361 aaatataaat gtaaaaatat agtaatattg gaggtcgaac gatgagaaat aaaaagaaaa
      421 aaataaatag aaagaagttg tatttgcttt taagtgctat tgtagtgtgt ctggctttta
      481 tagtagttgg tgttcgcgtc agctctttaa atatgaagga aaatgaaatt agtagaaatg
      541 caaaactgtc aagttctact attacagata tagtatctaa tacatcaata gaagttagta
      601 aagggccaag taaaagttct ggaaaaatag catacataac tatagatgat ggcccatcta
      661 aatttactga cgagatgata aaaactttaa ataaatacaa tgttaaagca acttttttta
      721 tgatagatgg gaatatgaag gaatatccac agcaagttaa aaatataatt aaaaatggga
      781 acacagcagg ttttcatagt gtatcacatg atattcacaa gctttatgtt actagtacat
      841 cagcaaaaga ggaatttgat acaaatgatc aaacttttta taagataact ggtaaacatt
      901 caaaagtaat aagaatacct tatggaagca aaccatatac accacaagcc tcttatcagg
      961 ctttagttga tgctggatat aaaatttggg actgggactt agatacagaa gattggagat
     1021 ctaattcttc tcaaattgta caaaatgtaa aaaatcatat taagaataga aaaggtgaag
     1081 ataaagacca acttgtagtt ttaatgcatg agaaaaaaca gagtgcagaa gctttagatt
     1141 cagttttaaa attcttatca gatgaaggtt atgaatttgc gccagtagat caaaaccaaa
     1201 taccaaaaaa ttattggtta cgtaatttag agtagttaaa atataagaat attgattgat
     1261 atttttagaa attgagaata aaaatgagtg aggtgtaggt atattgaata aaaaacttta
     1321 tataggaatg gtcggaatat tgtctttgat gatggttggt tgtaataaaa gtttagcaaa
     1381 agtaaatgat gtggaaatca caaaagaaca gtacaaaaaa acaaaagcag ttttatctgc
     1441 tactaataat tatataaatg ggcagtcttt agatgaatta gaaaaaactt tggataaaaa
     1501 aggtagaaat aaattagaaa atgttataat atcgtttatg gtggataatg aacttttata
     1561 tcaagaggct aaggataaag gactaactcc aagtaaaagt gaagtagact caaaatatca
     1621 agaactagaa gataagatga atttgaatac aagctataag gaaaaaatgg acaaagcagg
     1681 tgttgataaa gaatatttaa aacaagaaat atctagagat ttagcaatag ataaaaataa
     1741 aaaggccttt gaagatagaa taaacataag tgataatgat atggaagcct actatacaag
     1801 tcataaaaaa gattttaatg tagaagaagt aagtgcttct cagatactta tatcgacttt
     1861 agataaaaat aaaaaagaag taagcaaaga taaaaaagaa gctttgaaga agaaggcaga
     1921 taatatattg actaaaatca aaaatggaga aagttttgaa agtttagcta aaaaatattc
     1981 ggatgataaa gcaactggaa aaaatggagg acagctagga tattttacaa aggatgataa
     2041 aaatgctgag tttacaaagg aagtgttcaa attaaagaaa aatgaagttt caaatgtgtt
     2101 tgaaactagt tatggatatc atatagtcaa agttactgat aaaagagaaa gacaaaaaag
     2161 ttttaatgag tgtcagagtt taataagaga atctatttta aatgaaaagt atattgagca
     2221 tataaaaaaa ctgaatgaag atgctaaaat agacagatag aatttctaac agctcaatcc
     2281 tagcctgtaa tcggagggag attttacagg aatgtttaag agtttaaaga gaacgaaagt
     2341 tgagaaatac attatcgata ataaagactc tttttataga atagcatata gttatacgaa
     2401 gaatgaagaa gatgccttag atgtggttca agaagccatg tataaggcac tatactcagt
     2461 ggaaaatatt aaagaagtaa attatataaa gacatggttt tataaaattt tagttagaac
     2521 gagtatagat tttattagaa aaaaccgtaa atataataat atgacagata ttgaccttat
     2581 agatgagact ggagagtatg ataaatatac tgatttggat ttaagaagag ctttggaaga
     2641 attacctatt gaatacaaat ctattataat attaagattt tttgaagatt taaagattga
     2701 agaagtagca ataatattgg atgaaaatgt gaatactgtt aaaactaggt tatatactgc
     2761 tttaaaaaaa ctaaaattaa aaattgaaga gtagggataa aaagaatgac aagcaaagaa
     2821 aggttaaata agttaaaaga tgagtatcat aaaattccaa tacccaagaa attagatact
     2881 ataataaatc atgaaaaaat agaaaacata tatcgagaga aaaataaaaa agtaaataga
     2941 ttaagattta aagttgcaat agcctttgca tgtatattta cagtattagt aaatataagt
     3001 cctgtatttg cagacaattt ttctaagata cctgtaattg gagtaatagt agaagtaata
     3061 actataaaaa attatagttt aaaaagtgaa aattatgaag ctgagattga tattcctaaa
     3121 atacgaggtt taaaagataa aaacttagaa caaagattaa acagtagttt tatggaagat
     3181 ggaaaaaggc tttatcatca attccaagag agaatggaga agatacaatc atcaaagaat
     3241 aaaggatata aatctttaag tttgagttat agtgtaaaaa ataattctaa aaaatttcta
     3301 tcaatagaga tgacaaaaaa tgagatagag gcttcatcat atgtgagtaa ggcacattat
     3361 actattgata aaaaaagaca aatagttttg actttgccaa tgctttttaa agatgataag
     3421 tatataaaag ttataagtga taatataaaa gaacaaatga gagaacaaat gaaaaaggat
     3481 agtacaaaat cctattttat agatcaaaaa aaagacttgc ctgttgaaga ttttaaaaca
     3541 attaataagt atcaggattt ttattttaat aaaaatgaag accttgtaat ttgttttgat
     3601 gaatatgaag tagcacctgg ttatatgggt gcagttgagt ttgtaatacc atataaagta
     3661 ataaaagatt tgtaaattga atagtagttt atatatacat attttaagaa ggacaatatg
     3721 actacatata aaaagcttaa aaaatctata tttaaaattt ctatattttc aatatgctta
     3781 ttagtctcat tagtagatat aaaccctata tttgcaggta gttcctctaa tgagacttca
     3841 aatagtgata cgataaaggt gttaaatgct aagagtctca actttaaaca aggcaattat
     3901 gaagtgaatg tgagtattcc taaaataaaa ggtttaaaag ataaaacttt agagaataaa
     3961 ttaaacaatg agtttttgca agatgggaaa aaaatttaca gtgaatttca aaataaaatg
     4021 aaaaaggaaa ataataaggg tcatagatac ctaaatgtaa tccataatgt caaaaataat
     4081 tctaaggact ttttatcaat agaaatgaca aaagaagaga tagaagattc ctcgtctata
     4141 actaagaagc attacactgt tgataaaaat agaggaatac tattaaattt accaatattt
     4201 tttaaagata gtagatatat tagagctata agtgataata taaaagagca gatgagaaat
     4261 caaattaaga aagatgatac aaaatcttat tttatagacc aagataaaga cttcccagtg
     4321 aaagattttg aatctataaa taagtatcaa gatttttatt ttaataaaaa tggaaatctt
     4381 gtgattagtt ttgaagaata tgaagtagta cctggttata tgggagcagt agaatttgtg
     4441 ataccaaata aagtaataaa aagactaaga tagatatata gaagattttt agatagactg
     4501 gtaaggagaa ttatatgaat atatatgaac aagcaataag attaatagaa aataatgaag
     4561 atttcgcatt tgctacaata acgtctcatt caggttcaac accacgtgaa actggagcaa
     4621 tgatgattgt gaaaaatgat tctactatat ttggttcagt aggaggaggt agtgtagaag
     4681 cagaatgtat aaaacatgca attaatgtta taaaaaatag agaatcaatg ttgtataaat
     4741 ttactctaaa caaatctgat gtggcaaaac taggaatgat atgtggaggt actggagaaa
     4801 tacaaataga ttttatagat agtaaattaa aaagtaatat agaaaaattc aataagagac
     4861 taaaggaaaa tacaagtaaa gcatatatat ttggtgcagg tcatatttca agagatgttg
     4921 ctgtgatact atctctttta gaatttagaa cagtagtaat agatgataga gaagaatttg
     4981 ctaattatga aaggttccca gaatcagaag tagtagtttt agattctttt gaaaatatac
     5041 cagattttcc aactgacgaa aatagctata taattatttt aacaagaggt catctatatg
     5101 attcaagtgc tttagaatgg gctcttaaaa gagaaactgg ttatataggt atgattggaa
     5161 gtagaacaaa aattggattg acgtacgaaa aactcatgaa aaaaggtttt aagaaggaag
     5221 aattatcaaa agtacatgca ccaataggca taaaattgaa tgctcaaaca ccagctgaaa
     5281 tagctgtatg tatagctgct gaacttataa attgtagagc taataaggaa aaataaacta
     5341 atgaacacag ggagtaaaga tattgatact ttaaatgtta attttagagg gattgaaata
     5401 aaagcagttt tagtatataa aaatagaaaa aatatttcta taaaaataga tccatttggc
     5461 aaaataattg taatgagtcc tcctaaaata tccaaaaaaa taataaaaga tataattatt
     5521 gaaaaaggag actggatact taaaaagtca gaaaaatata aaggaagaga agaagtatac
     5581 aaacaaagaa tgtttataac tggcgaaaaa tttctttatt taggtgaaga gtaccatcta
     5641 gttataaaag aactgttgaa agattctgtc aaaaaaagta actgtagaat aactattaac
     5701 gaatatcaaa tagttgttca gacaaatgat acaagtacag attttattaa aaaatcttta
     5761 aaatcttggt ataagatgga aagtgaaaga acagtattag agagaataga ttttcttaaa
     5821 ttaaattgtg aaataatgaa gcaattagtt ccagcaagtg ttaaggtaaa agaacaaaat
     5881 aagagatggg gaagttgtac agctcaaaaa aatatttata taaactcaaa aatatctatg
     5941 gcaagattag atgtaattga ttatgtaata atacatgagt ttagccattt agtacatatg
     6001 aatcactcta aaaaattcta tgatttagta aaatccatta tgcccgatta taaagataaa
     6061 gaaaattggc taaaaataaa tggatataaa ataataatct gaaaacgatt attccaaaat
     6121 acaggcgtaa agtataaaag aagtattatt gtgaaaaaaa taaataatct gaaaatttta
     6181 aaaaaagtca ttgacattgt aaatgatagg gaatataatc tatattaaca acaaaacaga
     6241 attaaaatat aaaattaaaa aattaaaagc aatgacagag acaacaatac taatagtgtt
     6301 atccacagag aaccaatgtt gctgagaatt ggtgttaaca gtagtataat gatcactctg
     6361 gagcttttgt actgaatatt tttagtaagt acaaacgtat gtcaacgtta catgactaag
     6421 gttaaggtaa ttaattaccc gacctgaaaa aagaggggta gatgtatttt ttatatctac
     6481 aaataaggtg gtaccgcgga atataacttt cgtccttata agtaatattt atattgctta
     6541 taaagatgaa agttttttta ttttaagcga tataaataaa tttaatccat ataaaattaa
     6601 taaaaaaata ataaaaataa ggagggattt ttatggaaaa tactgtatat gctaaattaa
     6661 accaaggaat agattctttt ttaagagtgg ctatgactct aagaagaaga gaggttgata
     6721 tacaatcaat aagtatgact gtagataata tgaataattc aggtataaaa ttaatagtaa
     6781 atgaagaaaa gacatcttta gattctgtct taaaccatat gaaaaaacta catgatataa
     6841 gagagataac tgttgaaaga atgagacatt aattaaatat attttataaa gctaaagata
     6901 aatattaaat aagaaaaatc ttagattaaa atataagtgt cattgaattt atcagtttat
     6961 cacagttaat aaatttatta gtttgttgta gctaataaaa actgaaaatt ctaaaattat
     7021 aattgtaaga ttatataaat ttagaattta aatctgttga ataatattaa aacaatgata
     7081 atgagtatga atttctaata aaaataaata tcataaaaat aagaaaaagt attgggagga
     7141 aatgaaaatg gcaagaatgt attatgaaaa agatgtggat ttagaggttt taaaaaataa
     7201 aaaggttgca gttttaggtt atggaagtca aggacatgca catgcacaaa atcttagaga
     7261 taacggtgta catgtagtga taggtcttta tgacggaagt aaatcagcac aaaaagcaaa
     7321 agaagatgga tttgaagtta agagtgtagc agaagctaca aaagaaagtg atttgacaat
     7381 gatgttaatg cctgatgaaa aacaaaagaa agtttatgaa gagagtgtaa aggataactt
     7441 aaaagaaggt cagacattag cttttgctca tggattcaat atacattata atcaagtaca
     7501 accaccagaa tttgttgatg ttgtaatggt agctcctaaa ggacctggtc acttagttag
     7561 aaatgtattt acaaagggaa gtggagttcc tgcactattt gcagtatatc aagactacac
     7621 taaaaaagct acagagacag ttttagcata tgcaaaaggg ataggagcaa caagagcagg
     7681 tgttttagaa actactttta aagaagaaac agaaacagat ttatttggag aacaatcagt
     7741 actttgtggt ggaataagtg aacttataaa attgggatat aaaacgcttg tagatgcagg
     7801 ttatcaaaaa gaagtagcat actttgaatg tttacatgaa atgaaactaa tagtcgacct
     7861 tatatatgaa ggtggatttg aaagaatgag atatagtata agtgacacag cagaatatgg
     7921 agattatgta tctggtaaaa gggtaataac tgatgctgca aaacaaggta tgcaaaatgt
     7981 tttagaggat atacaaaatg gaaaatttgc aaaagcgtgg attaaagaaa atgaagaagg
     8041 acgtgaaaac ttcctaaaaa ctagagaaga agaatataat acagaaattg ccgaagttgg
     8101 tagaaatcta agaagtatga tgtcattctt aaaataaata tatattaaat ggattagttt
     8161 gagggaaaga gtggtgtata gtagtgcgta tgaatggagc aaaggtaatt ttagaatgtt
     8221 taaagaaaga gggaattgac acaatatttg gatatcctgg tggagccgtg atacctcttt
     8281 atgatgctct atacgattat tcagatgatt ttaaacatat aaggacatcc catgaacaag
     8341 gtttagttca tgctgcagat gggtacgcaa gaagtactaa tacagtagga gtttgtttta
     8401 caacatctgg tccaggagca acaaatgcca taactggaat agcaacagct tttatggatt
     8461 cttcacctat ggttgtaatt tcagggcagg tgccaactag tttattagga aaagattctt
     8521 ttcaagaaat tgacataact ggagcaacat tatctatgac aaaacataat tatttagtta
     8581 gaaacactaa agaattagtt cctacaataa aagaagcttt tagagttgca aattcaggta
     8641 gaaaaggacc tgtacttgta gatgttccaa aagatttatt tttagcagaa atggatttta
     8701 gtggtgagga ctatgattta tgtcaaatag atgattatat ggattataag agtgattttg
     8761 atctagatga tgaaactaat attaagcttt taaatgaagc aatagatatc ataaaagaat
     8821 ctaaaaagcc tgtaatatat gcaggtggag gtgttaaatc ttcagacagt gaggaaattc
     8881 ttgaaaaatt tgcaactaag atagatacac ccgtactaaa tacacttatg gggcttggaa
     8941 atatagatag aaagaatgaa ctatctcttg gaatggttgg aatgcatgga agtagagaaa
     9001 gtaatttagc actttctaat tcagatttag taatagcgat aggtgcgaga tttagtgata
     9061 gagtgataag taaaagttct gaatttgcta aaaatgcaaa aataatacac atagacatag
     9121 acccatctga aataagtaaa aatatagaat ctaatgtatc tttggttgga gatgtaaagc
     9181 tagtccttag tttattaatt gaaagggttg aaagtaagaa taatagcaat tggaaagaag
     9241 agataaagac atttagaaag agtgagggtg tacaaacata tgaatttcat ccacaaaata
     9301 tacttaaaaa aataaatgaa aaatatgaaa cattaaagaa acccactgtg gtagtaactg
     9361 atgtcggtca acatcaaatg tggtctgcta aatactggaa ttttaaaggt aataaaagtt
     9421 ttataacatc agcagggtta ggaactatgg gatttggttt aggtgcagct attggaacta
     9481 aagctggtaa tgtagataaa aatgtggttt tagtaactgg tgatggaagt tttagaatga
     9541 actgtaatga actagctaca gttgcaaatt ataatgtacc tatgcttatt ttactactca
     9601 ataatagaac attaggaatg gtaagacaat ggcagaagtt attttccaat caaagatact
     9661 ctcaaactga tattaatgaa aatgtggatt atgttaaact cgtaaatgca tataatattg
     9721 atggatataa ggtatccaat atggaagaat taggaaaggt tctagatgtg atagatttta
     9781 ataaacctgt atttttacaa tgtgatatag ataaggatta tgatgtttat cctattgtag
     9841 cacctaatga tgcattggaa aatttgatat gtaactaaaa cttatactga atattgataa
     9901 aattttttat taaattttta ttggtattca gtttttgttt agagatagat tggcattttt
     9961 tatttttgca aataaatcaa tgaatatata attaagaact tattaaattt taattataca
    10021 atttagaata gtattacaac ttaagtaaat attaagtttt attcgttaga tttattatta
    10081 ataccttata ttagttatgg ttgttatata ctctaaagcc ttgaaattac aagcgtatag
    10141 ctgactatat aaatttgata attttagctt ttaaaataga taaattagat tttgccaccc
    10201 atttgccacc gtattatatt tttgggtggc aaactctaat ttgtaatttt ttcaaatata
    10261 tctacagttt catttttcat tttatcagtt acatgtgaat aggtatccat tgtagttgat
    10321 aattggctat gacccaaacg gttttgtatg tctttaatgt tagcaccatt ttctaataat
    10381 agagtagcat gtgcatgtct taaagaatga aaatggaagt cattgtttaa agctactcga
    10441 atttgtctta ctatagtgtc taaagtgtga gtattcactt gttgaccatt ttctttggta
    10501 catacccaat cactatcaaa gtaaaattct ccgtatttta atttcatttt cttttgatat
    10561 aatttatgtt cttttaatgc ctttattaaa gtgtcacctg taaatatagt tctgcaagag
    10621 ctttctgttt ttggttgccc taattcaaac attccatttg gttttttaat caaagtatgc
    10681 tttactgtga tagttttatt atcaaggtct atattatccc attttagtgc aataatttca
    10741 cctcttcgca taccagtatg aaatccaatt agtaaaacta tacgttgaaa tgaatcttga
    10801 ggaaatatat ttagtatttg attaaattct tctaatgtaa tagttttaac tttattagtt
    10861 tctgttttag atttagtttt tggtatgctt acatattgca tagggttttc tcgtatgtgt
    10921 ttgtaaggat ggactgctga ttttaatgac ctatgtaata tggcttttaa tacttgtaat
    10981 gtattttgag aataatcctc tttgtacttt ttatttatga agttttgtag tattgcagga
    11041 gttaaagctt ttactttgta agctcctagt tttggcttta tatgtttttc tatgtttatt
    11101 cggtagcttt cttgagtgtt gtatttacag ttgagtaaaa catattcttt gtaccaaaag
    11161 tctaggtagt ctgataagct gatgttactt tcttcaaaga ctatgccaga gttttcatat
    11221 tcatttagtg cttctcttag tgctttttct gcttcttttt ttgtattgcc accaactctt
    11281 tctacttttt ttctttttcc ttctactatg cctaggtcaa agtagtaata ccatttgttg
    11341 cttctttttc ttactccacc tttcataata gtatccctcc cttttagaat gtatgtttgt
    11401 ttggtgttta tataaaaaat gtctcctata tttacaagaa gacattaatt atattcgtca
    11461 aataatattt ctaatttaac ttttatacat tcatcacaat tattttcttt gttaacaaat
    11521 ttaataatgc aattatactt taatcctcta tttattatac ttgttaattc tccactataa
    11581 tatctaggaa tgtaacctat atgattctct tttttatcaa ataccttgat tgcatgagga
    11641 tcaaatttat tttctggttc taactctaga actagagctt catttgattc taattctatt
    11701 gaattatcac attcatgtcc ttcacaccca gcataatgtc ttactcctgc aatataaaaa
    11761 tatctaatta tatttttatc aatgtccaaa ggaattggat ctacaaattc tagtgtatct
    11821 ataggtaatc ttgcaccact tctttttaaa agttcatatg tatcatactc agacatatca
    11881 tacttatcca aaattttatt gatgtccttt ctttttttat caggtaatct acttgcaaat
    11941 gggctaaata aatcttcact ataatatgtt ttactaatat catcaaatga tattaaagca
    12001 ttaaaacctg ctttaatagc ttcatcaatt tcatattcat aagaaaattc atacaatcca
    12061 tttttagcta actttccaat tataaattgt cttctacttt ttggatcttt ccaaataaca
    12121 tataaaaaat cttttccatt tttaattgac attttatccc tctttcacaa attatatata
    12181 ttttttattc ttaacacttt ttcttttaga tatattttta gcaatttttt taactttggg
    12241 cttataatat tttctgaata gttatctatt aatgtattta tgtttacttc attcataaga
    12301 tttattatct tttctacaaa atctatagtt tgttcataat aatttgaatt taaatatttt
    12361 agcacttcta agtgtgttgg ttctttttta ttttttccat ctatccttat tcttgatctt
    12421 gaacgtgtat ttataagagc ttcaaatctt tgatgatcag tgaagaaaga atctatgttc
    12481 tcaatattaa cataacaaca taatgatgat ccgttatcat atagaggact aagagtaatt
    12541 ttattttgac tttctagtag agcccaatta ttttgatgcc tatctctatt tccaatcaag
    12601 taatcaaaaa ttaacatttt tagaaaatca ttttttatgt tatattcttc tatagattta
    12661 aaaatcattt tcaatgaata gtattcatta tttttataat catataactt atctgtatta
    12721 taacaaggat aatatttgtt tattaaccac acgccttcaa tcaatttctc atcattttta
    12781 ttgattaaat aactcattga accaattctt ttattatatg tacctatctc aacattagca
    12841 caaggaatct ttaacaaact agctatttca caggctaatt tttctgccca atattctcca
    12901 gtttcttttg ttttagggaa tttaaacaag cctatatctt tagttttttc attaattagc
    12961 cagattttct cgcttcttcc actaccataa gaaaaaccat catactcttt ccattcatca
    13021 aaatttttaa tcacgaaaat aatcctctca aacaagtatt ttatattctt attgtatcat
    13081 ttgattctaa atttgcaaga attttattat ccataataag cttaacttaa aataaaaatt
    13141 ttttctagat tttatttatt ccacttttaa cttcaagctt tattacatat ggaatatgtg
    13201 tttactcatc aaaattacta ctgtgtataa tggttttact ttataaatac ttaattttag
    13261 ttttctatat ttatttggta atttttttga gtgttgttaa gtaagaaata ttttttgtac
    13321 caaaaggtaa gctgatattg ctttattcag atactatgcc taggtcaaag tagtaatacc
    13381 atttgttgct tctttttctt actccacctt tcataatagt atctctccct tttagaatgt
    13441 atgtatgttt ggtgtttata taaaagagca gattaactgc tctttatata cttttgttat
    13501 gtacaatatt atcttaattt tgtttatctg gtttttactt aattattttc ttaatattct
    13561 tacaattttt tagcttttat gtgtagttta ttataataat attgaaatat atatttatta
    13621 agtaaaaaat ctataaaagt agaataaatg agtttaaaaa atatcctcat atatgtagaa
    13681 atttggcaaa ttagttaata ctaaaaatct attatttcct aagtccaaca tgcttttctt
    13741 ttttgataga aattctaatc tttttagtaa gaagttgatg ctaacttgta attcttcagc
    13801 tatttcgtat acacttgtag cgtgtgaatt aataacatgt attatttctt cttctgttat
    13861 aagaaattca catgcccatt ttagtgcttt gttttcagtt ttgtctatca agattttatt
    13921 tttgtaactg ttttttgaag atacatagtt tccaacactg gtaaaatgat gtcctaattc
    13981 ttctgctaag atttctatta gtttagcatt gttttgtttt aatgaattaa gtaatgatat
    14041 aatttttagt ccttgtctgt ttatatacaa tccttttatg tcatctgcta ttttgtcagt
    14101 gtagtaaatt tctatctctt cattatttgc taagtctaaa agtgcgtcta gtttgttcat
    14161 tgaaatcccc ctataaaaag aatgtatgtg ttactttttt atatataaag agcaggagtg
    14221 aactgctcta aatattcatt ttttatatct atcaactaaa aactctatgt agctattaat
    14281 atgttcttgc gcttctttag gtaaactttc ataagattta attattttgg atatttcatc
    14341 agtagttatg taattttcaa ttgaggtttt accaagtaga taatcaactg acacattaaa
    14401 aaattctgaa attcttaaca agtcttcttt aacaggttct cttttgctag tttctaacat
    14461 agcaattttg cttagtgaac aattaagtaa ttttgataat tctgcttgag ttaattcttt
    14521 tttctttctt aattctttta accttattgg aaaagttgaa tttaagcatt ttgttaattt
    14581 atttgaatca tctatgttaa tttttccaat taagtaatct gtagatactc caaaatagtc
    14641 tgagcaatct ttaataaatg attcttttgg ttctcttaac ccattttcaa ttctggataa
    14701 agtggattta tttatattta agtctgtgct taatttatct aaagaaattc ctttttcagt
    14761 tctcaatttt tttaatatta acatagattc aatccttatt ttttatattt attcattaaa
    14821 aattcaatat aatcgttgag ttgttcttga gcttcctctg gtaaatcttt gtgaggatta
    14881 actctgtgtg cggctacagt atcgatgtga tttctaacaa gagttctacc caaaagataa
    14941 tcaattgata catcaaaaaa atctgcccaa ttttctagaa tatttatttc tggttttctt
    15001 ttattatttt catattgcga tattgttgat ttattaaaac ttgtaaagta aactttattg
    15061 aattttgaaa ctagttcatc ttgagtaagt tttttctctt ctcgtaattg tttgaaacgt
    15121 tctccaaaag ttgccacaaa caacacctcc caataattaa atgatactat aagtttacag
    15181 aaacgtaaac ttatgtttag aaaaaaataa aaaagttaac aaaaaaataa acttatgtat
    15241 tgacaagttt tgtttgtgtg aatataataa aagtatgaag ttcacaaaaa taaaacattg
    15301 gaaggtgaag aacatgaaga aaagaagttt aaaagctggt cgaatagaag caggatatac
    15361 ccaagaagaa ctagcatata agataggaat agcaaaatca acatataatc taaaagaaaa
    15421 tggaaaaaga aattttacgg aaaaagaaat gataatgatt agctatattt taaataaaac
    15481 aatggatgaa ctttttttag aaaaaaggtt aacataaatt aaaatttatt gttcacaaaa
    15541 acaatcataa ggaggaaaga aattatgaat aatttacaaa tattcaaaaa taagactttt
    15601 ggagaaataa gagtaatcga actaaatgga gaattttggt ttgttggaaa agatattgca
    15661 gaacaattag gatataaaga tacatctgat gctttgaaaa gacatgttga tgatgaagat
    15721 aaaggagtag gtgaaatacc gactcctggt ggtaatcaaa atatgaaagt aattaatgaa
    15781 agtggtcttt attctttaat attaagttct aagttgccaa gtgcaaaatt atttaaacgt
    15841 tgggtaacta atgagatatt accaagtata cgtagtactg ggacatataa tatgatagat
    15901 ttgcaaacta aactaccaac tacatataaa gaagcgttac aacatcttat agagcaagta
    15961 gaagtaaatg agcagttaca actagaaagt aaaatgaaag accaagtaat aaaagaacta
    16021 aaaccaaagg cagattatac agatatgata ttaaaaaata aaggacttgt cacaataact
    16081 caaatagcaa aagactatgg aatgagtgga aaagaaatga ataaaatact tcatgaaaga
    16141 gggattcaat ataaacaaag tggacaatgg cttttatata aacaacatca agggaaggga
    16201 tacactcatt cagaaacaat agacataact agaagtaatg gaatgcctga tgtaaaaatg
    16261 acaactaagt ggactcaaaa gggaagattg tttttatatg atttattgaa aacaaataac
    16321 atattaccag atatagaaaa aggatatagt tatcaaactt caatattagg ttagtacttt
    16381 gaaaactaaa tacagaatat tttagaaagg agagattgaa ttggaaaata agaaaataga
    16441 agaaatagag aaaagaataa ccatgcttga aaatcaattg caagcacagt tatttagtca
    16501 gataacagct caatcaaata gtgattttat agattatatg taccaatgta tagttttgta
    16561 tttacatgag atgaataaaa gtgaaaaaag aaaaattagt gaaattacta aagaaagaat
    16621 cagaaaataa gaatgataat tacaatataa aacctattct tctagcaggt tttgaagggt
    16681 cagttttctt tactgaagtt atcataaagt ttatctgtga tgtgtgttga agaatttcag
    16741 aataaactcc atttactatg ccgtagtagt gaattagaca agggttatga taacctactt
    16801 cttcaacaat cattaagaca tcttttccaa agttagtcaa ttttagtgca atttcatgtt
    16861 catcatcaag ttctttttca aattcctcta tagatttaag aataatttta tattgaaatt
    16921 cagcatagta aaaatactct gtgttagaag tatcagagac tgtctttctt atactttctt
    16981 gtaattcttt agatatattg tttgaaaact gcataataca ccatcctttt gaaaattttg
    17041 gaatttattc catgtttata ttataccatg tagaactgag gtgaatacaa tgttaatagg
    17101 cgacaatata aagcaaatgc taagaaaaag agatataaaa ccttataaat tggcaaaaga
    17161 attgaatata gatgtaagtg gtttatataa attactgaga aataaaagtt ctaatccaac
    17221 tatagatact ctaataaaat tagctgatta tttagatatt acattagacg aattagttgg
    17281 aagataaata attaaaaaga ggggagagaa ttttcacatg gatatttctg agagtatagc
    17341 aaaacaattt agtgatagtt taaaaagttt aatagaaata gagataagta aacaagagac
    17401 agatagagtt aagagtcaaa ctattgaaca aaaggtaaag gtactggagc ctaaagatat
    17461 agttgtttta ataaaaagag gttatccaaa ttatctaata acagtagaag aagcaagggg
    17521 aattttaaag ttagatacag cttttatgcg taggttagta agcacaggtt tgataaaatc
    17581 actagttaga ggtgatggta gaaaaatttc aagatatgaa gttgatgatt ttattgaaag
    17641 aaatcaaggt aaaaatttgg atgaactttt aaaagcagta gagtgggggg atgaaattgc
    17701 tgagtcttga tactaataat aagaatgtag taactcttaa aaaagatgga aaagttatag
    17761 cagatatagt atttaaagat attaaaactg gtaagaaaat atcggttgga atattaaata
    17821 aaaaagtgtt ggtaaaataa ccaacacaca aaaaaataaa aaataaaata taacacaagt
    17881 aaagtataac acaaaaagga gatatttaaa atggttgaag ttaatgtaaa tgtaaaagtt
    17941 aaagtagaag caccagaatt tacaaatgca ctattagtag tggcgaatgc tttaggaggt
    18001 cttaatctag gaaaagcaat gcagatagaa cctataaata taacagatat gaaagaagag
    18061 aagaaagttg aagttaaaaa agctgaaaag attaaaaagg tagaagtaaa agaagacgtt
    18121 aaagaagaaa tagcagaagc aaaggaagaa atagaaaaga ataatgaaaa tacaactagt
    18181 gaagttaaat acacaaaaga agaggtaaga actaaggcag cacaagtaag taaggcaggt
    18241 aagaaggata agcttaagga gttatttggt gaatttggag ctagtaagtt aagtgaagta
    18301 aaagaagaag attattcagc ttttatgaac aaattagaaa gtttataggg ggtacataaa
    18361 tgccattaca acatgcaaga cttagtgcga gtggagccca tagatggctt cactgcactc
    18421 ctagtataaa attagaagaa aactatccac cattaactag tatatatgca gaagaaggaa
    18481 cagttgcaca tgaattagca gaagttaaat taatgctaga gtatgaaaaa ataagtaaaa
    18541 aggcatataa tgcaagaatt aaaaagatac agaaaagtga gtattataac tctgaaatgg
    18601 aggactatat acaatcttat gttgagaatg tagttgaatt agtaaatgat agtaaagcta
    18661 tatgtgatga tgtaatagtg atgttagaag aaagactcga ttttagtgag tgggttccag
    18721 aaggatttgg aacaggagac gttgttgtaa tatctgatgg tatacttcaa gttatagacc
    18781 taaagtatgg aaaaggttta gaagtttcag ctatagagaa tcctcaactt agactatatg
    18841 gtttaggggc atataatcag tttgaaatgc tatacgatat tgatttaatt aaaacaacaa
    18901 taattcaacc tagacttgat aacatatcaa gtgaagaaat agaagttact aaattactta
    18961 catgggcaga taatgttaag aagaaggctc aaatggcttt taatggtgaa ggagagtttg
    19021 taagtggcag tcattgtgga ttttgtagag ctaaaaatga ttgcagaaaa agagctgagg
    19081 ataaccttaa actagctaga aaatatgatt ttgctgacac atttgctctt aataaatatg
    19141 agatagcaga tattttaggt tttgctaaga atatacaaga ttggttaaag gatgttcaaa
    19201 gttatgcact agaacaagct gaaaagcatg gtgttaaata tccaggatat aagcttgtag
    19261 aaggcagaag taatagaaag tatgtagatg agcaagaagt tgctaaggtt ttattaaatt
    19321 cagattatga tgaagaaaaa atttataagc caaggacctt aaaaggaatt agtgatatgg
    19381 agaaagctat aggtaaaaag agttttgcta aactactaag tgatttaatt ataaaaccag
    19441 ttggaaaagc tactttagta gtagaaagtg ataaaaggtc agaaattaat agtatagatt
    19501 cagcgaaaaa agattttgaa atataaatgg gggatattac atgccattag atgcaggtat
    19561 aaaagataaa ttagatgaaa gcataagtat aattttagat ttaaaggata gaaaaaagag
    19621 tatcgaaaaa gatttagatt atgaaaaaga taatttacta gatatgttaa agagactaaa
    19681 tattacagaa tataactcaa ctgaagaaaa tgcaaaagtt atagtgatgg attttaaaag
    19741 agaaagttta ataaaggata aagtgatgtc aactttcctc gaagtaaatg acaatcatct
    19801 tgatagagtg tatataccag aacatataaa agttagtcca gtatgctttg tatcagtaag
    19861 agcaaaggat aattaaaaat atttaattcc tagtagctat aaaaagattt aaaaggggat
    19921 ataattttat gagtaatcaa gaaataacta aactagttga aaataatttg aatcttgtac
    19981 attttagtat aaatagattt tttaagtctt atgtagagca acatccatac ttatatgaag
    20041 agttttatca agaaggatgt attgggttat ataaagcagc attaaattat gatagtaaca
    20101 gaggcaagtt ttctacagtg gctgtttctt acataagaat agaaatgttt aaagctttgc
    20161 gttatggcga aaaacattat agaaactcta tagaaggttt agatgtaagt gtatttgaag
    20221 atagtaaaag accattaaaa gatatacatg gattttatga aattcaattt aactatgatg
    20281 ttgattatat aagaaaaatt gcaagaaagt catatttaaa agatattgat aaaatagtag
    20341 attggatttt acaagataaa aagatatctg atataggaga gttattaaat gtttcttcaa
    20401 cagttatttg tggaagaatt aaattgttta gtaagcaagt tagagattta gataaatttg
    20461 gagaaataac ttcaaatata aaatcaatat accaaatata aaaatattag gaggaagaaa
    20521 attatgagta attcagtaca atcaacaaag gtagtaacag gaaaggtaag attaagttat
    20581 tgcaatatct ttaaaagcag agcaatggta gaaggtgcag agcccaagta ttcagtttgt
    20641 attttaatac caaaatcaga taaggtgact ttaggaagaa taaagaaggc tattgatgca
    20701 gctaaagaac aaggtaaaac ttctaaatgg ggtggaaaat taccaggtaa tttaaaaaca
    20761 cctcttcgtg atggagatgc agaaagagct gatgaagcag aggaatatgt aggaatgtat
    20821 tttttaaatg caaatagtac acaaaagcca ggaatagttg ataaagactt aaatgaaata
    20881 ttagataata cagaggtata tagtggttgt tatggaagag ttagtataaa ctttttccca
    20941 tataatagtg caggaaataa aggaataggt tgtggcttac aaaatgttca aaagttagca
    21001 gatggagaag tacttggagg agctagagct agtgcagaag cagatttctc agatgacttt
    21061 gaatatgaag atgaagaaga ggacttctta agttaatgag gaccttatca attgatatag
    21121 aaacatatag tgatttagat ataaaaaaag ttggagtcta cagatatgta gactctgcta
    21181 attttgagat actgttattt gcctatgctt ttgataatga agaggtaaaa gttattgatt
    21241 tagtaaatga tgaagagtta ccaaaagaag taatagaagc tttaaatgat aataaagtta
    21301 taaaatcagc atttaatgct aattttgaaa gaacagcaat aagtaaattt ttaaatatta
    21361 atttaaaacc aaatgagtgg tcatgtacaa tgataaaggc gttaacacta ggacttccag
    21421 gaagtttaga tagtgtgtct aaggctttaa agtttaatga agataaacag aaaatgaaag
    21481 aaggtaaagc attaatacaa tatttctgta aaccttgtaa ggctacaaaa gttaacaagg
    21541 gaagaactag aaatttacca atacatgata tggaaaaatg gaataaattt aaagaatatt
    21601 gtaaacaaga tgttgtagtt gaaagagaaa taagaaacaa acttagtaag tataagacta
    21661 ccgaaagaga aattaaatta tggtatttag accagagaat taatgatact ggtattaaag
    21721 ttgatacaga gttaatagag aatgcaatag aatgtgataa aagatatact gaaaaactta
    21781 caaaagaagc aattaaaata actggtctaa ataatccaaa tagtccagct caattaaaaa
    21841 aatggttaag tgataaagtt ggctttgaga ttacaagcct aacaaaagaa agtattccag
    21901 aaatattaaa acaagttgat gatgaaaatg tagttagaat tttagaactt agaaaattaa
    21961 tgtccaaaac ttctataaag aaatatgagg ctatgaaatt agctaaaggt aatgacaata
    22021 gagtaagagg tctactacag ttttatgggg ctaataggac tggtagatgg gcaggaagat
    22081 tagtacaagt acagaattta ccacaaaatc atatagagga tttagaccta gctagaaacc
    22141 tattaaaaga aggagatttt gatttaatag agcttttata tgatagtgta ccagatgtct
    22201 taagtcaatt gataaggaca gcttttatac caagtgaagg tcatagattt atagtatcag
    22261 attttagtgc aatagaagct agggttatag cttggttcgc tggtgagaag tggagattag
    22321 atgtatttaa ttctcacggg aaaatatatg aagctagtgc tagtcatatg tttaaagttc
    22381 caatagaaag tattaaaaaa ggtgacccac ttagacaaaa aggtaagatt tcagagctag
    22441 ctcttggata tgggggaagc ataggagctt taacatcaat gggggctata aaaatgggtc
    22501 ttgatgaaga ggagcttcaa cctcttgtaa caacatggag aaatgctaat ccaaatataa
    22561 ctaaattttg gtgggatgta gataaggcag ctaaaaaagc cataaaagat aggactatag
    22621 taaaactcca acatgggatt aaatttattt ataatccagg agttttattt atagaactac
    22681 caagtggtag aagattatca tatctaagac ctaagataga gcctcatact acatttagtg
    22741 gggataagat aacatatgaa ggtatggaac agacaagtaa acaatggaaa agaatagata
    22801 cttatggacc taagttagtt gagaatattg ttcaagctac agctagagat tgcttaagag
    22861 aggctatgtt taatgtgaca gatgcaggtt atagcattgt aatgcatgta catgatgagc
    22921 ttgtaattga tgtagataaa aaaggaggtt ctttagaaga agttaacagt attatgggaa
    22981 aagaaatatc ttgggctaaa ggtcttcctc ttaaagcaga tggatatgaa tgtgattatt
    23041 ataagaaaga ctaggtgatt gatttatgga tataaaggcc agtgaaattg aacacataaa
    23101 tgtaagacat gatggccaac tcatgctggc cataggaaaa aataaattag aaacacattg
    23161 gaaaaataaa agtattttat ggtccgaact tgtaaataga ttaagtaaaa cattaagaac
    23221 tcaagaaaca tatacagaat ataggaagat gtcaaagact gaaaaagata gagttaaaga
    23281 tgttggtgga tttgtaggtg gaagcttaaa aaatggaaga cgaaaagcag agaatattgc
    23341 aaatagaagt attataactt tagatataga ttatgcgaat aaagatatat gggaggatat
    23401 aacattatta aatgattatg cttgtcttat gtattctacg cactcacata ctgagaataa
    23461 tcccagatat agacttgtaa tacctttaac tagaccagta ctaccagaag aatatcaagc
    23521 aatttccagg atgatagcag atactatagg aatagatatg tttgatgata ctacatatca
    23581 acctcataga cttatgtatt ttccaagtac ttcaattgat ggagactata tatttaaatt
    23641 tcaagatgga gagtttttaa acccaaatga aatactagat ttatatttag actggacaga
    23701 tgtaagctat tggccagaaa gttcgaggga gaggcaaaag tttaatacac aattaaaaaa
    23761 acaacaagac cctattgaaa aggctggaat tataggtgca ttttgcaggt cctatagtat
    23821 aaaagaaact atagaaaatt tcttaaatga agtatatatt cctggtattg atgaaaccag
    23881 gtatacatat tcagaaggta gtacaagtgg tggagtagtt atttatgatg acaagttttc
    23941 atatagtcat catggtacag acccagcaag cggaatttta tgcaatgctt ttgatttagt
    24001 taggatacat aaatttggtg aacttgatga agatgctaaa cccgaaacgc ctgtaaatag
    24061 attaccttca tttactcgaa tgagcgaatt tgcaagcagt gacactaaag tacgaaagac
    24121 tataggaaga gaaaaccttg ataaagctaa ggatgatttt ggtgatatag attttgaaga
    24181 tgatgaatgg ttaactaggt tagattatga caataaggga agttataaga aaacaacaaa
    24241 caatatctta atgtttatag aaaatgaccc atatttgaaa ggaaaaatag cttataatga
    24301 attttcaaat agagctgtcg ttttaggtaa gttaccttgg agaaaagatg ataaattaaa
    24361 tgattggaat gatagtgatg attctgggct tagacatcat atagaaacaa tttacaatat
    24421 ctcatcacca tcaaaagtaa atgatgctct aataattgct tttgaaaata atacttttca
    24481 tcctataaaa gattatttaa attctttaaa gtgggatggc attaagagag tggatacact
    24541 tttaattgac tatttaggtg cagaagataa ccactataca aggactataa taagaaaagt
    24601 tttagtagca gcagtagcaa gagtatttaa tccaggaata aagtttgata atatgatggt
    24661 tttatctggt cctcaaggaa tgggaaaaag tacttttatt aaaaaacttg gtggagactg
    24721 gtattctgat agtttaacta ctgtacaagg taaagaagcg tatgaacaat tacaaggagt
    24781 atggttgctg gaaatgggtg aaatgatggc tactaaaaaa gcagatattg aggcagttaa
    24841 gcattttcta agcaaatcag aggatatata tagggtcgca tatgggaaga gaacatcaag
    24901 gtttttacgt caatgtgtag ttataggaac aactaatgat aaagaatttt taagagataa
    24961 gaccgggaat agaaggtttt ggccaataga tacaggagta aagaaggtta acaagagtat
    25021 atttaatggt caacttgata atgaaagaaa tcaaatttgg gcagaagcag tagaattatg
    25081 taaagccaat gaacagctat atctatcaga tgaggagaaa aaagaagctg aaagacaaca
    25141 aagaactcac tcagaagaaa atgctaaatc tggaattatc gaagagtatc taaataaacc
    25201 tattactgag aattggtatg atttaagtat ttcagaaaag agagaatata ttcatggttc
    25261 agattttggt gatttaaaag aaggaacaat attaagagaa aaaacgtgtg ttatggagat
    25321 atgggttgag ctatttaatg gagaacctaa acaactcacg cctatcttat caagagaaat
    25381 taatgatata ttaaaaggat tagatgattg gatatcatat aatggaaatt taaggtttgg
    25441 gaaaatatat ggtaggcaga gagcttatgt acgtaaaagt tagtgtcaac agaattaaaa
    25501 acagagtaaa tttgaaaatt ttaaaagtgt caacaatgtc aacaaaaaaa atggctttgt
    25561 tgacgcttat gttgacacct aagatgcagt aattacaatg tttgaggtat taatgtcaac
    25621 aatgtcaaca aaaattaagt ataaagtagt ataagtaata ttaggcatat acgtatatac
    25681 atatgtatgc ctaatatgta tatatacata tatatagaaa atctgttgac attgttgaca
    25741 cttaaatttt aaggaagtga tattttgtta gaatcaaaaa tagaaaaaag acttaaaaaa
    25801 gagattgagt tgttaggtgg aaaggctatg aagtttattt caccaggtga ggcaggtgtg
    25861 ccagatagga ttgtcttatt accagaagga catgttatat ttgtagaact taaagcacca
    25921 ggtaaaaaac taaggaagct tcaacaatat aaaatgcgag aattaagaga attaggattt
    25981 aaagttaagt gtgtaagcac attaaaagag atagatgatt ttatcaagga ggttaaggga
    26041 tggaatttaa accacatcca taccaagaat acacaattag aaaaactata gataataata
    26101 atataggttt actattagat atgggattag gtaaaacagt ctgtactcta acagctataa
    26161 gtgaattaat gtatgattac tttgatatat caaaagtttt agttatagca cctttaagag
    26221 ttgcaagaga tacctggagt agtgaagtaa aaaaatggga acatctaaag catctaaaag
    26281 tatctaaagt tcttggtagt aaattagata gagtgagagc tttaagtaca gactcagata
    26341 tttatataat aaatagagaa atggtaccct ggatagtgga tttttataaa agaaaatggc
    26401 catttgatat ggttgtgata gatgaactta gttcttttaa atcaaataaa gcacaacgat
    26461 ttaaaagttt aaagaaagta ttgcctttaa ctaaaagagt agttgggctt actggtacac
    26521 caacaccaaa tagtttaata gacttatggg cacagatgta cttacttgat agaggtgaaa
    26581 gactaggtaa aactattaca ggatataaag aacgatactt tgagccagga caaaaaaatt
    26641 atcaaacagg agctatatat aattggcaac ctaaaaatgg agcagaaaat gcaatacata
    26701 ataaaataaa agatatttgt ataagcttaa aagcagaaga ttatttgaat atgcctagaa
    26761 aaatagataa taaaattgaa atacaccttg atagtaaaat acttaaatat tacaaagaat
    26821 tagaaaaaga gaagatatta gaattagata gagatataat aacagcttca tcagcagctg
    26881 tagcagcaaa taaactttta caattagcta atggagcaat ttatgataat gataaaaatg
    26941 taaaggaact ccatagagaa aaattagaag ctttaaaaga aattatagat gtttcaaatg
    27001 gtaagcctat tatagttttt tataactata aacatgatta taatcgttta atgaaagagt
    27061 ttaagagttt gaaaccaaga acaatagaaa actcaaaaga tatatatgat tggaataatg
    27121 gaagaataca attattactt tgtcatccag caagtacagg acatggactt aatttacaat
    27181 caggtggaag tataattgtt tggtttggat taacttggag cttagaactg tatcaacaag
    27241 ctaatgcaag gctttataga cagggtcaaa gggaaactgt tattatccat catctaatct
    27301 gtaaaggtac aatagatgaa caagtaatgg aagctataga gaataaggat aaagggcaaa
    27361 gtgcattact tgaagcagta aaagcaaaat taaaagaata tagggagtga aggtatgaaa
    27421 gtaaaaagaa aagatggaac ttataagccc aggtaaaata gtaagatgtg cttggtgtgg
    27481 tagaagattt tataagttgg ataaatctaa agttgagtat tgcagtagaa gttgttcgaa
    27541 gagagcaaga aggagtgatt taagtggata aagaaagtat aaaagaaatt gtaagagagt
    27601 taagaaaaga agaaagagaa gataggaaaa aaggcgtttt atataatact agattattga
    27661 tgaagcatta taatgatttt aagagacata tagactcagc ggtaagtgaa gctaaagatg
    27721 tagattactt agaggatgat ttaggtaagc ttgataatga agagttgtat attttaagta
    27781 taaagaaaag taaagcaaga actattatta tgatagcaca tatagattca gcaatgaaaa
    27841 ctttgaaatt aagacaagag cgtttacatt cttatgaaaa gtatagagct ttagagcttt
    27901 actattgtga tgaaaaaaca tatgaagaaa tagcagagat attaaattgt ggagtagtta
    27961 catcaagacg ttggattaat gaaatggtaa aagaacttgg agtgtattta tttggtatag
    28021 atggcttgaa attaactgtt tgataaatac atgataaaaa gatgatattt taatgatatt
    28081 taacccctgt tataatggta gtatgaaaca agtgtataat atattccccc ttgaaaaagg
    28141 ctaagttacc ccaacttagt cttttttatt ttgtaaataa ggagaatgat aaatgagtaa
    28201 ttcatgactt agagtgattt ctaggtcttt ttttatacaa ttttaatctc tcatattgaa
    28261 gggtcgaaaa taagggtgtt caaattgaat attctttatt aataactaat ttaggaggta
    28321 gtagtatgtt gaaaatttta caagagaaaa atgtaaggat gatatggtca aaaaatggag
    28381 aagaagtttg gtttaatgca aatgacgtag gagaggaact aggcatagta aatattcgtg
    28441 atacattaag aaatatagat agagaatata aaaagaaatt taatgagtct actgtcggag
    28501 attcctacac tagaaacttt aaagataaat tgcctaactt tggtactact tttgttacag
    28561 aagaagctgt gtacaatatg tcatttagaa gtaataaagc agaagcaaaa ctatttacga
    28621 agtgggttac aaaagcactt aaacaaatta gaatacatgg ttactatatt gccacagaaa
    28681 aagatcaaga atggctggat ataagaacag aaggtaaaag gtcagaaaag attttacaga
    28741 tgaaatacaa gagttttttt attaatacaa gtacttgaaa gagttatatc tatgaaatta
    28801 cctaggttga tagataaagg tatgaattat aaagaggtat ataaaaagat taaagaatta
    28861 atcgaaatga tttaaatgac tgtattaata gagggtcttt ttttttatgt aatgaattaa
    28921 aaatgaaata tatttgtaca ttatagacag gaataaggta aaagtattat ttagacttat
    28981 tttgtggttg tcgaatgatt attgaaggat attgactgat tatatagaat tatatcatta
    29041 taaatatttg tgaaaggaat aatggtataa tgagtaattt taaaaaaaga tatattgatg
    29101 tttatagaat agatattata aataaaatta atgggagtgt ctattcagat gaagaaattt
    29161 taaaagaggt aataaagacc ttctttaagg ataaagaata ttgtaaagaa aacaaaaatg
    29221 gaattaatat tactaaagat aaaaagtttt ggattacaga agtaaaatgt agcgaggatg
    29281 atttaataat caaggtgaaa cttgaatata ctaaatacaa tcagaataca aatattataa
    29341 atgctcatac taaagaagta gagggtaata aaaaaatgga tcaaggagat agcaataagc
    29401 agcacctatt tattaaattt atgaaggaaa ataatatagc agtagtatta tttgaaagag
    29461 tttttgtggg ggttcccatg attgaactaa gtaaaaactt acatctatat tatgaggata
    29521 tgattgaaaa taataaattt gataataata tgtcaaaaac atcaattaat attacacaaa
    29581 tagcaagtaa agattttata gaacaaatat tagatttaga tggtgtttca aaaataactt
    29641 taaatgttga tagagagaag tttggaactg atgaagataa tttgttttct aatttaaata
    29701 attcaagacg acataacgat ttgatatata agcccacatt tagaactaga tatttaccta
    29761 gtgaagttaa aagatgttgc gaaaaatata ttaaaggaga ggaatttaga aataaaaaaa
    29821 taaataggat aataattgag ggagagaaaa ataagagaaa attaaagttg gatactgagg
    29881 gtataaagtt gtgtagagaa atagaggttt ctcttgatat agataatcat atagatagtg
    29941 aagacatttt tgagaagatg aataatttga tagatgattt aattgagaat aattcagttt
    30001 tttttaattt aatgtatcaa gaagttgcta tgagtgaagc atagattgct aaggaggtgt
    30061 taaaatgtat aaaaaggtat taaatatctt actcaattat tacaaggcta taaattctaa
    30121 agaagtagta gagttagttt taacaccagt attaatatgt attatttcat acatgttttt
    30181 taattcgtca attaataaag aagttgtaat ttctttaaac aaagatgtac taactttgtc
    30241 aggactatta gtagcttttg gagtttgcat tattacatta ttgtttacta catataacaa
    30301 atcaatatca gaagcaaaag atataaaaac agaaagaaag gtaaatggat ttaatatatc
    30361 atactttcaa ttcatacagt taaaagcata ttatactgta ataatggaga ttttggttgt
    30421 tgtaatatgt tttatcaata caataatgct tactcgatat tattctaata taattttcta
    30481 tgttttaata ttttttacag ttcatataat tttatctcta actacattaa taataagtat
    30541 gtttcattta tcctggaagg atagaggaga ctagatttga aacaatccta ttaaatagga
    30601 ttgttttttt attcccaaaa caaacaaata aagaggtggt gatgtgcaag atgtcaaaga
    30661 aaaggtaaaa caagattact taaaaggaat gaaacaaaag gaaatatcag caaagtatga
    30721 cattagttta aatactttaa agtcatggat aaaaagatac aattggtcta gtgaaaaaaa
    30781 gaagggtgca cctataaata aaaggggtgc acccttttct aataaaaatt cagttggtta
    30841 tggtgctcca aaagagaata aaaatgctga aaagtttggt ttcttctcaa aatacttacc
    30901 tgaagaaact agggaattaa tacaagaaat atctataaaa gataaatttg atattctttg
    30961 ggaacagata acaattcaat acgcagcaat aataagagca cagaagataa tgtatgttaa
    31021 agataaagag gaaatgatta aggaattaaa gaaacatgaa atcacagaaa atagtgagaa
    31081 gatagagtat gaatttcaat ttgcatggga taggcaagca tcttttctta atgcacagag
    31141 tagagctatg agtgagttaa ggagtttaat taagcagtat gatgaaatga ttcataagga
    31201 ttggaatttg gctacagagg agcagaaaac aagagttgag aagttgaaat gtgaggttgc
    31261 taacctaaat aaagatgata ttggagatga tgagttgaaa ataagtgtag attatggtga
    31321 tagaaatgat agttagaata aattttaatc cagatttcaa ggaagctaat tttactaaaa
    31381 aaagatacag agcaatgaaa ggttcagcag ggagtggaaa atctgttaat gtagcacaag
    31441 actatatact aaagttagga gataagaagt atcaaggagc taatctatta gtagttagaa
    31501 agtcagaagc tacacataag tattcaacgt atgcagagct tacaggagct ataaatcgta
    31561 tttatggtaa acaagctgat aagtattgga aaactacttt aaatccttta gaaattaaga
    31621 gtaaagttac tggtaactct ataattttca gaggagttaa tgatgcaaaa caaagagaaa
    31681 aattaaaatc aattaacttc tcgaaaggaa aattaacatg ggtttggtgt gaagaagcta
    31741 cagaacttat ggaaagtgac atagatatac tagatgaccg tttaagaggt attttaacta
    31801 atcctaactt gtattatcaa atgacattta catttaatcc agtctcagct actcattgga
    31861 taaaaagaaa gtattttgac tataaaaatg atgatatatt tactcatcat agtacttatc
    31921 tacaaaatag attcatagat gaagcttact acagaagaat gcaaatgaga aaagagcaag
    31981 acccagaagg gtacaaagta tatggtcttg gagaatgggg agaaactggt ggagcaatac
    32041 ttaaaaatta tgttatacat gaatttccca cagaatttga atactttgac aatatgagac
    32101 tgtcacaaga ctttggcttt aatcatgcaa atgcaatact tagaattggc tttaaggatg
    32161 gagagttata tatatgtaat gaaatatatg tacatgaaat ggatacctca gaaatcataa
    32221 aaattgcaaa tagtagaggt ttagaaaaga ctctatttat gtactgtgat agtgctgaac
    32281 cagatagaat taagatgtgg aagagtgcag gatataaagc taaaggagtt aaaaaaggac
    32341 caggaagtgt taaagctcaa atagattatt tgaaacaatt aagaatacat gtacatccta
    32401 gttgcactaa taccataaaa gaaatacaac aatggaaatg gaaacaagac gaaagaactg
    32461 gattatatct tgatgaacca gttgagttta tggatgatgc aatggctgct cttagatatt
    32521 ctatagataa taagcttaaa aataatggaa taagcttctt aaagtaaagg aggtgttaaa
    32581 tatttatata agtgaaacag atttaataaa agttcagtta aaaaaagaga gcacctttaa
    32641 cctagtaaaa gtcatagaac actacatctt aaagcatagg ccagaaaaat ataaacaagg
    32701 agaagaatac tattatggta atactgatgt aaacaataag agaagatatt atctcttaga
    32761 tggagctaag gttgatgatt ttactaaagt taataataaa gcaattaaca actaccataa
    32821 gcttttagtt gaccaaaagg taggctatag tgtcggaaat cccatagtat ttaatgcaga
    32881 tgatgataat ctcactaagc ttttaaatga cttactagga gaagagtttg acgatacaat
    32941 aacagaacta tatctcaatg ctagtaataa aggggttgaa tggttacatc catatattaa
    33001 tagaaaaggt gagtttaaat atgtaataat tccagctgaa gaagcaattc ctatttggga
    33061 tagtaaaaga cagagggaat tagttgcatt tattaggttt tattatattg aagatataga
    33121 tggaaataaa ataaaaagag ttgagtacta cacagaaaat gacgtaactt actttattga
    33181 aagaggtaat agttttattc aagaattttt atatgatgaa tatggaaaaa tgactgatat
    33241 acaagaaggt cattttagaa taaataacaa agaacaggga tggggtaaag ttccatttat
    33301 accttttaaa aataatgaaa agtgtgtctc agatttaact ttctataagt ctttaattga
    33361 tatatatgat aataacattt ctacacttgc agacaattta gatgaaatgc aagaggtcat
    33421 ttatgtatta aaagagtatc caggaacaag tctacaagag tttatagata atataagata
    33481 ctataaatca attaaagtag atggtggagg tggagttgat aaactagaga taaatatacc
    33541 agttgaagcc aaaaaggagc ttcttgatag attagaaaag aatataatta tttttggtca
    33601 aggagttaat cccgaatctc aaaacacagg tgacaaatcg ggtgtagctc ttaaattttt
    33661 atattcacta cttgatttaa aatgttccaa gactgaaaag aagtttaaaa aagcaattag
    33721 agagctttta tggtttgtgt gtgagtattt aaagataagt ggtagtaaga gctatgatta
    33781 taagtcagtt caaataacat ttaatcattc tatgataata aatgagtctg aaaaaataga
    33841 tatggcttct aaatcaattg gaattatatc agatgaaact attgttagta atcatccttg
    33901 ggttgaagat gttaatgacg aacttgaaag acttaaaaaa caggaagaaa cccaaaaaga
    33961 atatgatgat ttaattccta ctaataatca agagggtgtt atagatgaaa cataaagatt
    34021 attggaggaa aagatttgaa caattagaag aagttcaaaa caataaaagt gtaaaatatt
    34081 atcttgaatt ggaaaagaaa tacaaattag ctatgaatag tatagaaaaa gatatattat
    34141 catggtataa cagatttgct gaaaatgaag gaatatcttt attagaagct aagaaactgt
    34201 taaatacaag agaattagaa gaatttaagt ggaatgtaga agagtatatt aaatatggca
    34261 aagaaaatgc tataaatcaa aagtggatga aggagttaga aaatgctagt gcaagacttc
    34321 atataacaag gcttgaagct ttaaagttac aaatacagca acacgtagaa gttttatatg
    34381 gaaatgaact tgatggtatt gataaactaa tgagagatat ttatacaagt ggatactatc
    34441 atacagcttt taatgttcaa caaggagtaa acgttggttg gagtttaatg agtcttgata
    34501 ctaatagaat aaataaaatt atctctaaac catgggcaac agatggatta aactttagtg
    34561 aaagaatttg gggtaagcat agacctactt taataaatga actacatact aagctaactc
    34621 aatcaattat tagaggtgaa aatccaaaaa atctagtaaa tgactttgct aagagattta
    34681 atgtatctaa atcacaagct aagaatttga taatgactga atcagctttc tttgcatcag
    34741 caagtagaaa agattgtttt agtgatttag atgtagagaa atatgagatt attgctacat
    34801 tagatttaag aacttcaaat atatgcagag agttagatgg aaaaatattt gatatgaaag
    34861 attatcaagt tggaataaca gctccaccat ttcattgtcg ttgtaggaca acaacagctc
    34921 cttggttcga ggatgaagaa ggctatagag cagcaagagg agaagatgga aaaacatatt
    34981 atgtaccatc tagtatgaag tataatgagt ggtatgagaa acatatagag ggtagacttg
    35041 gaaaagaaaa agctgacacc cttagaaaaa tgcaactaaa tgagagtaaa gatagaaaac
    35101 aatttgaaga atataaaaag gtattaggaa atgaaatacc ttctaaattt gatgaatatc
    35161 aacatatgaa gtataatgat actataaggt atgaggaagc taaaaagctt tataaggatg
    35221 taaattggca agttaagaat cagagaaatt taactagtgg aagtgttcat tcagtgccat
    35281 ttgaatcaaa accaaatagt gtatttgata attacaaaga tggtaaatta attcaaagaa
    35341 gatactatgg taatacagga aaacctagat tagatttaga cttaactgac catagaaacc
    35401 caaagcaaca taaaattgta cctcataaac atgattggtt agctgatgaa aataaccatg
    35461 ataaggtaaa aagagagaag gatatggagc ttacaaaagc tcataaaata gctaacaaag
    35521 atattttgaa tggagaataa aatggataat aaatttaaga atttacaaca tttaattgat
    35581 tctattgatt taggattaga tatagaattt gacttatata ataaaccata taatatatct
    35641 attggagatg atgatacaag gtttattact ctttgcccta atggagatac aaagtattat
    35701 aaaaatggta aggatatggt tgataattac aacattgatg gtaaattact aaaggattta
    35761 tggaaagata tacaaatagt aaatatgtaa aagcacttgc taaataataa attagtaggt
    35821 gcttttattt tgtaaaaaat gaaaggagaa atttaaaatg gattggttaa aagaattgct
    35881 agaaggaata aaagtagagg ataacaaaat tgatgtagct tctcttcaaa aatctataga
    35941 aaagaaaata aaggagacta cagttactca agaagattat gtaaatcttg aaacacaact
    36001 taatacagct aatgaaacta ttaaaaagtt tgaaggaggt atgacaaaag aagatgtaga
    36061 gaatctaaaa acaacttatg aaactgataa gaaaactttg gaagaaacct acaaaaaaga
    36121 aattgaagaa aaagatttta attactggtt aggtgatgct tttaaatctg ttaaatgtag
    36181 agatgaaata gcgttaaaag ctcatttaga catggaagcc ctaagaaata gtaaagatag
    36241 acaaaaagct tttgaagagc aaataaaccc tttgaaacag gataaagatt atttgtttaa
    36301 tgcaacacta gaaggtgaag aacctaaaat agatactata acaccagggc aagagcctaa
    36361 gataaatgat tttggtttta attttactgg ggtaagacct catgaaaata ataataaata
    36421 ggaggaaata aaatggcagc actaaattat gcaaaagaat attcaaatgt tttagcacaa
    36481 gcatatcctt atactttaaa cttcggggat ttgtatgcaa cagaaaataa tggaagatat
    36541 agatggacgg gttctaaaac aatagaaata ccaactatat ctacaactgg aagggtagat
    36601 tcaaacagag atacaatagc agtagctcaa agaaactatg ataatgcttg ggaacctaag
    36661 gtattaacta atcaaaggaa atggtcaaca ttggttcatc cagcagatat aaaccaaact
    36721 aattatgtgg cttcaatagg taatataaca aaagtatata atgaggaaca aaagtttcca
    36781 gagatggatg cttactgtat atctaaaata tatgctgatt ggaccgcatt aggtaacaca
    36841 gcagatacaa ctgttcttac aacagcaaac gtattagaag tatttgataa gttaatggaa
    36901 aaaatgacag aagctagagt acctgaaaat ggaagaatat tgtatgttac tccagtagta
    36961 aatacactta tcaaaaatgc aaaagagata caaagaacag taaacataaa agatggtgga
    37021 acttctttaa atagacaaac cacagatatt gacacagtta aaataattaa agtaccatct
    37081 aatctaatga aaactgcata tgattttaca actggatgga aagtaggagc aggagctaaa
    37141 caaatcttta tgtccttagt tcacccaagt gcaataatta cacctgtttc ttatcagttc
    37201 tctaagttag acgaaccaac agcagttaca gagggaaaat acttctactt tgaagaaagt
    37261 tttgaggatg tatttatatt aaataaaaaa gctgatgcaa tacaatttgt tgttgaagca
    37321 tagaaaaagg agtgatatat agtgccacaa gtaaaaaaac taaatagaat actaaccata
    37381 gaagaatgta aaatagatga tttcttagag atgggatatg atttgataga tgaaactggt
    37441 aaggcagtaa agtatggcaa gtcattaagt gtaaaagatt taatagctga aaataatatt
    37501 ttaaggtcaa aagttgagtc tttagaagaa gaaaataagc agcttaaaga gaaaaataaa
    37561 cttactaaaa agtaggtgaa aattatggaa aataatataa ttgatgaaat agaaaaaaga
    37621 cttgaaagtt ttggatatat attaaaagat ggagataagt ggttaatagg ttttgtaaga
    37681 gaaaaaatag aaaatattat taaactagat tgtaatataa aaactatgcc aattgaattg
    37741 aaagaaattg aagttgatat gatagttgga gagttcttat ttaccaagaa aaatatgggt
    37801 caattagata tagaaagcat taactttgaa gctgtagaaa agtctatatc agaaggtgat
    37861 acaaaggtag attttgctat aggaagtggt tctcaaacac cagaacaacg ctttgatagc
    37921 ttaatagctt atcttactac ttatggtaag aataagatat taacctttag gtgcttaaga
    37981 tggtaagtaa aactagaaaa gcaatagaaa tgttatatag agataaatgt actatagttg
    38041 agtatcagcc aattaaagac cctgtaacaa aacgaactaa caataaagaa gtgattgtat
    38101 tagaaaatca accatgtaaa ctttcatata aaaatatagt ttctgctaca gaagggaaat
    38161 tagctaagct agagcaaact attaaactct ttatatctcc agatatagaa attaaagcag
    38221 gttcaaaact tattataaat gataaagagt atgtaagaag tggagaatca gctatatatc
    38281 caaatcatca agaaataata cttgagttat ttaaggataa agcataatgg ctagatgggg
    38341 cagtgttgat tttagagagt ttaaaagagt ttgtaaaaag atggagaaat ttacaaagat
    38401 tgatttagat aagttttgca aggatgcagc aagagaatta gcagcaagac tcttaggaaa
    38461 agtaattaga aggacaccag ttgatacagg atttttgaga gagggttgga gtggagtagc
    38521 ttatgctagg tcgcttcctg tttacaaaca aggtaataat tatattatag aagttgttaa
    38581 tccaactgaa tatgccttaa cacaatgggg catataaaac ccagcaaaat cggtaaacgc
    38641 taagtgtaat agtgttacat tataaagtag gtagaatatg gacatagaat aactcttgat
    38701 ttataatgta taaaagaggt gattttatgg caaaattttt agatattaca ggaaaaaaat
    38761 ttggaaggct aagagtcatt aaattttcta aagagataaa aagcggtaaa agaaatagga
    38821 aatattggtt atgtaagtgt gattgtggaa atttcaaaga aataagaact gactctttaa
    38881 ctagtggctt agtgcaatct tgtggatgtt taaaaaaaga acaagataaa ttaaatttaa
    38941 cagacaaata ccaatttaaa aagaaatata aggttcaaaa taaaagactt tacagtatat
    39001 ggaagggtat aatatctaga tgtacagata aaaataataa aagatacaat agatatggtg
    39061 aaaggaatat aattgtgtgt gatgaatggt tttgctatga taattttgca aattgggcat
    39121 tgagtaatgg atattcagag aaacttacaa ttgatagaat aaataatgaa ggaaattatg
    39181 aatcaagcaa ttgtagatgg gtggatataa agacacaatg tagaaatagg tcaacaaata
    39241 tattagtaaa acatgaagaa aaagaaatta cattgataga gctttctgaa aaaacaggta
    39301 tttcatactc ttgcttaaga agtagatatt ctaagggatt agtaggaaat aagcttatag
    39361 aaaaagttaa gattattgaa gaaagcagag ctaagttatc tattgaggat gtaaaagaaa
    39421 ttagaaaaaa gtattctgat ggatatacaa taaaacaact aagtgaaata tatcctgtaa
    39481 catattcatc tatatcaaat atagtccata gaagaacatg gaaaaatatt taattaatta
    39541 tatatgccaa taccgaggaa aacctataga ttgcggatag gctataggtt tccgtagagc
    39601 gtagagagtg aataaatata atctctccaa gagtgctggg caactaaata aagtttattt
    39661 tgttgatgat gtacgccgaa cttataggaa actataagag ctagaggata aaaagccttt
    39721 aggataacaa aatgcatatg ttgaatttgg tcatagaact aaaaatggca agggttgggt
    39781 taaaggacaa catttcttaa ctatttcaga aatggaacta caaagtcaag ttgataagat
    39841 aatagagaaa aaactattaa tattgcttaa aggagtgttt gatgcttaat aatattatag
    39901 atggaatatc agtaaagtta gataaatcgt ttggagaaaa atatacaatt tatagcgaag
    39961 atgtggagca aggtattaat gaaccttgtt tttttattgt tcctttaaat ccaagcaaga
    40021 caccatatcc aagcgggaga gaattaaaga aaaattcttt tgatgtacat tatttccctc
    40081 gttcagaaga taagaatttt gaaataaatg agatagctga gatgctactg gaggaattag
    40141 agtatataga aattgatgga gatttagtaa gaggtacaaa tatgaatttt gaaattatag
    40201 acaatgttct tcacttcttt gttgattata actattttac tataaaaaat aatgatacca
    40261 ataagatgga tacagtagag ttattcggtg gtttgaagag aggtgataat tttgagtaaa
    40321 gcattaagca aagaagataa ctacaagttt actaaggagc agatagttaa ctctaagaag
    40381 tatgtaaata gaaaagattt attaaatgca attttaaaag aaaatgagtt atattccttc
    40441 tcagaggtag aggatagaat aaataaattt atgaaaggag tgagttagat ggctttaggt
    40501 ggaggaacat tcgtaacgca aaataaaata cttcctggtg catatataaa ttttatctca
    40561 gctaagaggg caaccagttc attatcagat agaggtattg ttgcaatgcc tttagagtta
    40621 gattggggca tagatgaaga agcattccaa gtaaccagtg atgattttga gaagtattca
    40681 gtgaagtatt ttggatatga ttatactcat gagaagctga aaggtttgag agatttattc
    40741 aaaaatataa ggttgggata tttttataaa ttaaataaag gcgttaaagc cagttgtact
    40801 atagccacag caaaatatag tggtataaga ggaaatgact taaaagtaac agttacaaca
    40861 aatatagatg ataatgctaa gtttgatgtt gtaatacttt tagataataa gaaggtagat
    40921 actcaaatag caaaggttat tacagactta caagacaatg actatatcac ttggaagaag
    40981 gatgcaacac tagaagcaag tgcaggactt gtatttactg gtggaaccaa tggcgaagct
    41041 gtgacaggag cagagtacca agctttcttg gataaaatag aaagttattc atttaatgca
    41101 ctagggtgtt tggctacaac aacagaaatt aaaagtttat ttgtagaatt tacaaagaga
    41161 atgagagata aggtaggagc taagtttcaa acagtactat ataagaaaag tgatgcagat
    41221 tatgaaggtg tagtgtctat agaaaataag attaaagata aagatttagt tgaatctagc
    41281 ttgatttatt gggctactgg agctatagca ggatgcgata taaataaatc taatactaat
    41341 aaaaagtatg atggtgagtt tgatgttgat gttaattata cacaaataca acttgaagaa
    41401 gctttaaaaa ctggtaaatt tatattccac aaggtgggag atgaagttca tgtgttagag
    41461 gatataaata cttttgtatc atttacagat gataaaaatg acgatttttc aagtaaccaa
    41521 agtgttagag tacttgacca aattgctaat gatattgcaa ctttatttaa tgaaaagtat
    41581 ttaggtaaag ttccgaatga taaggcagga agaataagtt tctggaatga tgttgttaaa
    41641 caccataaag aattagagaa tataagggca atagaagatt ttaaaactga tgatgttagt
    41701 gtagagcttg gaaatgataa gaaaactgtc atagtatctg atgctattaa ggtaataaat
    41761 gctatgagta agctttatat gactgtttca gttaattaga gaggagagtg ataatatggc
    41821 tcaaacaata aatgctaaag atacagttag tgcaaagaaa gctgaatgtt ttataactat
    41881 agaaggtaaa agatataatt ttatgcaagc tatagattta gaggctaaaa tggaaaaaaa
    41941 taaaagtgaa gttccaattc taggaagaac aacaaaggga aataaaacaa ctgggagtac
    42001 aaatactgga agtgcaacat ttcattataa tacttctatt tttagagaat tactttacag
    42061 atataaagaa actggtgagg atatttattt tgacatacaa gttacaaatg aagaccctac
    42121 atctgctgta ggaagacaga cagtagtact taaagattgt aatatggaca gtggaataat
    42181 tactaaattt gatgctgatg gtgagtattt agatgaagat atggatttca cttttgagga
    42241 ttgggaatta gttgaatcct ttgcattact taatggaatg ttataaaatt gaattatact
    42301 tgaaaaatat tatgatgtaa gtataaatta tttggaggta tttcatggaa tttgaaagtg
    42361 gaattaaatt aggcaaactt actttaatta aatatttagg gagaaataaa catagtaaaa
    42421 aagtctggct atgtcaatgt gagtgtggga ataaagttga gagagtagaa aataatttaa
    42481 agcaatcaat taaaaatgag aagcctccac attgtggttg ttctccaaac tggaaaggac
    42541 aaaataagag atttaaagat ataactggga aaacttttgg gaagcttaca gtattgaaaa
    42601 tgtctgggaa agataaatat agtcacaatt tatggttatg tcaatgtgaa tgtggaaata
    42661 gaacaataac tagtacaagt gcattagaac aaggaaaagc acaaagttgt ggctgtataa
    42721 gaaaggaaat attactagag cgctctacaa cgcataataa atctaatgat agcttatata
    42781 gagtttatca ccgtatgatt aaaaggtgca caaataagag taataaagat tataattatt
    42841 atggaggtag aggcatagaa gtatgcaatg aatggctaga ggattttata aatttttata
    42901 actggtctat agaaaatgga tatagaaaag gtttaacaat agatagaatt aatgtgaatg
    42961 gaaattacga accatcaaat tgtagatggg ttacttggga agttcaacga aataataaaa
    43021 gaaatagtat tagagttaat tataaaggtg aaatgattac tttaaaacaa tgtgcagaaa
    43081 atttgaatgt taagtatcta tttctttata atcaattagt tactaaaaaa attccattgg
    43141 aagaagtaat aaaaaatata ggtatggagt aaaatacaca tttataagtt ataaatgtgt
    43201 attttttata tgaaaaatta aaataaaagg agattagaat aatatgagta atttaagtgc
    43261 ttttttaagt caaaatgcaa taaaggttga taatgtaaaa tatgtagcga gtaacagatt
    43321 tttagataaa gaagggaaac cagttgaatg ggaattaaaa gttttatcat ctgaagaaga
    43381 cgaagcacta agaagaaagt gtactaaaag agtaaaagtg attggtaaca atggtaagca
    43441 tactggacaa tatacaagtg aaattgacta caatagttat gtagctgaat tatgtgtagc
    43501 atctacagta tttccagatt taaaggatgc cgaactccaa aatagttatg gagtaatggg
    43561 agaagctcag ttattaaaga caatgcttac agcaggtgag tatgtcaatt atacagtaaa
    43621 agtgaatgaa gtcaatggat ttgatacatc ttttgaggat aaagtagaag aagcaaaaaa
    43681 ctaatcagag gtggcgattt tgatgctagc atcactcatt attgtattca aaaattaaag
    43741 tggaagccaa gtgaatatat gaatttagaa gttaatgaga gagcgttagc agccgcctca
    43801 atacttataa agatagaaga tgaagaggaa gcaatgaaag aagctgaaag agagagaaag
    43861 aggggacgaa gaagatagca aaataaaaaa ataaatatag aataggtaaa atatgtaata
    43921 attatatgtt ataatatttt tagcaagaag atgtaatcta caatttatag agtggagttc
    43981 atactgggat aaaacctact tcctaatgaa aggaggtggg aagtatgaat aactttttac
    44041 ttaatgtaat agctggcgtt attgctagtt taatattttg cttaatttgt aaagtatttc
    44101 taaaagtaaa aagccactca actcgtggca agagtaaaag tggctgggaa tttgatttta
    44161 aaatcaagtt ccacaaattt aaataattac taattgttta aattatgaac ttcact
//
LOCUS       DAELSY010000015        42220 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.015, whole genome shotgun sequence.
ACCESSION   DAELSY010000015 DAELSY010000000
VERSION     DAELSY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42220
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.015"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(60..752)
                     /locus_tag="KPG09_000736"
     CDS             complement(60..752)
                     /locus_tag="KPG09_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0965362.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            complement(886..1602)
                     /locus_tag="KPG09_000737"
     CDS             complement(886..1602)
                     /locus_tag="KPG09_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0965363.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            1728..2462
                     /locus_tag="KPG09_000738"
     CDS             1728..2462
                     /locus_tag="KPG09_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis protein TtcA"
                     /protein_id="HBG0965364.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMIYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            complement(2747..3598)
                     /locus_tag="KPG09_000739"
     CDS             complement(2747..3598)
                     /locus_tag="KPG09_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0965365.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDTIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKNFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
     gene            complement(3624..4562)
                     /locus_tag="KPG09_000740"
     CDS             complement(3624..4562)
                     /locus_tag="KPG09_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG0965366.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            complement(4665..5315)
                     /locus_tag="KPG09_000741"
     CDS             complement(4665..5315)
                     /locus_tag="KPG09_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG0965367.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYIVENVESIKDILL"
     gene            complement(5465..5653)
                     /locus_tag="KPG09_000742"
     CDS             complement(5465..5653)
                     /locus_tag="KPG09_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG0965368.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            complement(5760..6944)
                     /locus_tag="KPG09_000743"
     CDS             complement(5760..6944)
                     /locus_tag="KPG09_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0965369.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            complement(7086..8003)
                     /locus_tag="KPG09_000744"
     CDS             complement(7086..8003)
                     /locus_tag="KPG09_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBG0965370.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            complement(8025..8753)
                     /locus_tag="KPG09_000745"
     CDS             complement(8025..8753)
                     /locus_tag="KPG09_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0965371.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            complement(8949..9149)
                     /locus_tag="KPG09_000746"
     CDS             complement(8949..9149)
                     /locus_tag="KPG09_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965372.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            complement(9274..9654)
                     /locus_tag="KPG09_000747"
     CDS             complement(9274..9654)
                     /locus_tag="KPG09_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0965373.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            complement(9927..11285)
                     /locus_tag="KPG09_000748"
     CDS             complement(9927..11285)
                     /locus_tag="KPG09_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0965374.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTIMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            11733..12185
                     /locus_tag="KPG09_000749"
     CDS             11733..12185
                     /locus_tag="KPG09_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HBG0965375.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            complement(12342..12959)
                     /locus_tag="KPG09_000750"
     CDS             complement(12342..12959)
                     /locus_tag="KPG09_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBG0965376.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFIYIYMGKEFGAKRNNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDREQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            13508..16168
                     /locus_tag="KPG09_000751"
     CDS             13508..16168
                     /locus_tag="KPG09_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HBG0965377.1"
                     /translation="MKKILKRLCTGFLAFATVVTALPSSTVHASNTQYWTESKERVSI
                     VEQVMNDGSISSTFNEGHLTVEGEDAYCIDINTAFKNGYKTKADASTRMSADQIADVA
                     LSIEYVKQYTDIHSGISSKHAYLLRQLVVWQRLSVQPDWQWDNVRASYDEIPKAVQDE
                     VFAGAKAFVKENKGRYDCGGYIYSGEGQELGQFWAKLAVGNTKLQKTSTNANITDGNG
                     IYSIAGATYGVYSDKDCTKQLTTLTTDTSGNTEAVEVRATIVYIKELSAPAGFKIDKT
                     VYSLSVEVGKTATLKVSDTPKVTDTLIELFKIDMETQKSNPQGNASLEGAEFTWNFYA
                     GYYNKNNLPAQPTRTWVTKTIAEKDSDGAIHYITRLADKYKVSWDSFYTQDGKNVLPL
                     GTLTVEETKSPSGYLLEGTYMQADGSEEQIKGMYLTQITEDGDLAVLSGSNQYHVSDK
                     VIRGGVKIQKRDLETKDTKAQGGATLKDTAFEIISLNENAVLVEGKLYKKNEVLKTIH
                     TDIEGIASTSADLLPYGKYRLSEQKPPEGYLTDGAKPIDFEITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGSHKRLADVPFRITSKTTGESHVVVTDDNGQFSTASDWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALLYDTYIIEELRCESNKGFKLIPPFEIVISRN
                     KVVVDLGTLTDEYEKEITIHTTATSKDGEKTILAGKDVTITDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIINDKRIENDYIFTADSETMEVKIEFTFDASNLGGKQLVTFEELYD
                     LSNPDEPIKVAEHKDIEDDGQTVTIKEVPETPTPEEPEKPTTPDTPTKTDSPKTGDNT
                     NILAFAIMMFVSAGGLAGTYFFKHRKMKKS"
     gene            16393..16716
                     /locus_tag="KPG09_000752"
     CDS             16393..16716
                     /locus_tag="KPG09_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG0965378.1"
                     /translation="MELKYIVPNVAQTFGNLEFAGENKVDQQRINGRYTLVSRSYNLY
                     SDIQRADDIIVVLPAKAGEKHFKPEQKVKLINPRITAEGYNVKGRGFTNYILMADDML
                     PAESK"
     gene            16731..17114
                     /locus_tag="KPG09_000753"
     CDS             16731..17114
                     /locus_tag="KPG09_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG0965379.1"
                     /translation="MRLTNGIVLDNEKTFGVLKFSALRHEVRVENEDGTTSDEIKRRT
                     YDLKCSVQERMIQVSIPADVPVKDFPYNAEVELINPVADTVANANFRGTTVDWYIKAD
                     DIVLKNKGGQAGNPQNNAPQGQQKK"
     gene            17210..17623
                     /locus_tag="KPG09_000754"
     CDS             17210..17623
                     /locus_tag="KPG09_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG0965380.1"
                     /translation="MLDKIPNAEEMTALVGQSLYSVWNELCALIDEKYEMECLWNKGG
                     KAWNYEYKYRRGGKTLCALYARENCVGFMIILGKDERLKFDTYRDNYSKEVQRIYDET
                     KTYHDGKWLMFEPTDTTLFDDFVKLLRIKRKPNRK"
     gene            17730..18188
                     /locus_tag="KPG09_000755"
     CDS             17730..18188
                     /locus_tag="KPG09_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0965381.1"
                     /translation="MKLKNPMLVVTDIDKSVEFYKKVFGLYVIMDFGANKTLTGGLAL
                     QTSETYKEFIGTSDISFGGNNFEVYFEEEDFDKFADRLKEYDIEYVHPIIEHSWGQRV
                     VRFYDPDKHIIEVGENMKIVCKRFLNSGMTPEQVAERMDVPMKFINACMR"
     gene            18213..18755
                     /locus_tag="KPG09_000756"
     CDS             18213..18755
                     /locus_tag="KPG09_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG0965382.1"
                     /translation="MKGFERKNQLFSLCGLNCGLCPMLLGNYCGGCGNGNQSCRIAKC
                     SLEHGQIEYCYECKQYPCEKYQHIDDYDSFITHKRRKADLERAKNIGIEQYNLEQQEK
                     TQILSYLLSNYNDGRRKTFFCVAVNLLELSELQEAMKQIQSNDELTLLPFKAQCLYVV
                     EVFQKIAERRNIKLKLFKKK"
     gene            18791..19318
                     /locus_tag="KPG09_000757"
     CDS             18791..19318
                     /locus_tag="KPG09_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HBG0965383.1"
                     /translation="MTNGGIPLETVELRFQYTQSEYIKAERQYLISSKTIHKYDIALV
                     AVFLLLSVVYMLFSSFSVFSILIFGLIIVVTALGSYLYILMPILKFKQTAKYHEEYTL
                     VFSKENIKFKTQSIDSEVKWDIYSALWESHDFYYLIQAPRLYTLIPKRIFKDLNEKQL
                     FEEIAQSRVKTTKHI"
     gene            19428..20822
                     /locus_tag="KPG09_000758"
     CDS             19428..20822
                     /locus_tag="KPG09_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0965384.1"
                     /translation="MRMILNKGHRIRARDKNLVYHFSIGTLLFVFVAVLLLLNIKQLM
                     RTDWEHFSLLDNGLTLSPYNFITILIATGICALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMENGLLHIHCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRISIDEVKAENGCLRLMKNLV
                     WEYDVLPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEDMIDCVNAFYEGMVQRSEEMKQHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAFVRQ
                     DGTATCEAKGDGTD"
     gene            21102..22223
                     /locus_tag="KPG09_000759"
     CDS             21102..22223
                     /locus_tag="KPG09_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBG0965385.1"
                     /translation="MAGISREHLSRIEAGKVALTEDMKHKLLEAVEKFNPDAPMFLLF
                     DYVRIRFPTLDIQHVIKDILKLNIDYMLHEDFGHYKYTEHYYIGDVFVYTSQDKEKGV
                     LLELKGKGCRQFESYLLAQERSWYDFLMDALVDGGVMKRIDLAINDRTGLLDIPELIE
                     KCKNEECISKFRSFKNYGSGELVKHNEANKDGMGHTLYIGSFTSEVYFCVYEKNYEQS
                     SKLGIPIEEVPIKNRFEIRLKNEHAYYAIRELLTHYDAELTAFSIINQYIRFADKEAN
                     KRKSDWKTNARWSWFIGEGRPPVKLTTKPEPYTLQRTLNWLQRQVAPTLKMLKQIDAG
                     NQMDYLKTIEQQAKLTDKHKQIIRQQTASVDELIEKEKE"
     gene            22230..22364
                     /locus_tag="KPG09_000760"
     CDS             22230..22364
                     /locus_tag="KPG09_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBG0965386.1"
                     /translation="MWELIKDLLLVSLGMGIGVVLMCIMSVGKEADRHMIELKESEEK
                     "
     gene            22365..22586
                     /locus_tag="KPG09_000761"
     CDS             22365..22586
                     /locus_tag="KPG09_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965387.1"
                     /translation="MNFGQNLYTWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALVAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            22906..23544
                     /locus_tag="KPG09_000762"
     CDS             22906..23544
                     /locus_tag="KPG09_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
                     /protein_id="HBG0965388.1"
                     /translation="MEYKYTKIDLNKWNRGKLFQSYIDNMRIVMSLTVDIDVTKFIEF
                     SKKHDLKFYPAMIWAVSKVVNEHDEFKYSWDNNGNLIKWDYISPSYTEFHKEDENFTK
                     IVTDFSDSIFEFHKRFILDREKYKTERAFVENQSLNFFDVSCLPWVKYNHFDVHVFDE
                     GKFLAPVITWGKYELEQERYIMPLTMNIHHAVADGFHLSRFFNEVQELINSI"
     gene            23621..23809
                     /locus_tag="KPG09_000763"
     CDS             23621..23809
                     /locus_tag="KPG09_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965389.1"
                     /translation="MKDIFSDMQASIGCTYISDLPHHKRMVWQEMKRLNLADYPQKQL
                     EDFAGYVFGVPYTSIAEA"
     gene            23830..24327
                     /locus_tag="KPG09_000764"
     CDS             23830..24327
                     /locus_tag="KPG09_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG0965390.1"
                     /translation="MNEMRVCIESRLHTSEEIRSAWFTLPIDEEELEEQIGVPIDSED
                     YHIVEKELPFADEVGEDATIEKLNDLYHTFESLPADLQEDYGELMCYFTSLDELHQHR
                     HDVIHYSWCKNMADVARHLLDNDPAFSAIEERLTRYFDYEAYGQYLDDNGEFIETDHG
                     IYELP"
     gene            24343..24846
                     /locus_tag="KPG09_000765"
     CDS             24343..24846
                     /locus_tag="KPG09_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG0965391.1"
                     /translation="MVEDMRVYIANLGKYNEGYLVGDWFSFPIDEEDVAERIGLNERY
                     EEYAVHDTENFPIEIGEYISIEELNEMYEMICELPDYITDSLDEFVSNYGSLEELYDH
                     RDDMILYAGCEDMTDVAYYFIDELQALGDIPPSLQNYIDYEAYGRDLSFEGTFIETRY
                     GMCEIPW"
     gene            24959..25348
                     /locus_tag="KPG09_000766"
     CDS             24959..25348
                     /locus_tag="KPG09_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG0965392.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDLPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITFALRPKITY
                     AGKAVKLDKQVLHEAITAVRSVSYVPD"
     gene            25335..27767
                     /locus_tag="KPG09_000767"
     CDS             25335..27767
                     /locus_tag="KPG09_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0965393.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSSRSIQEQSKKLVTGRLKDVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTDQSMNLKSVKQSAFLTFKEFIHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLDKNDFGYLIEHIYGRDGVAYEDYEYSLPKKKLKKE
                     TLITHYDLIRPTRCLVEESQRHLRLEHEDSESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALSTVRNKKKELKDLDNHAYQSGSETSSNVVDALDSVD
                     ELETDLDQSRESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYIGYSVDTGRNVYLQPS
                     LASQGVKDTVTNALASAFVESLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDRENAGLLDPFVIMQNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRAVTQSDQRGLLHVIEELRKEDTPISCNIADHIDSFTDYDFAHLLFSDGTV
                     QNAISLDNQLNIIQVADLVLPDKDTTFELLSVSMLIVISTFALDFIHSDRSVFKIVDL
                     DEAWAFLNVAQGETLSNKLVRAGRAMNAGVYFVTQSSGDVSKESLKNNIGLKFAFRST
                     DINEIKQTLEFFSIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFEELLHAF
                     DTRPPIQRNEVE"
     gene            27767..29902
                     /locus_tag="KPG09_000768"
     CDS             27767..29902
                     /locus_tag="KPG09_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG0965394.1"
                     /translation="MKERIKSVFTRKKIFRFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINTENLYSKYPLSNYQLDFYADNNWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     RLYLSNATGYVVQEAYKLDFINDMADSIGRSIQTLAGVTQNGFSTSGFYVGFLLLIIL
                     VVGIYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKVNDFSSDISTAS
                     LDLGTKIMLPDSDSKGKDSVDLIRDSLFSIQVQQPWLLLQFGNSGIEEIGADRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTILQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVYFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRLARAVSAGGVAGAVAGGVVASSKSTRQNGTKNNRGNTSSSMGQK
                     AGSAVGAVLDTKNKVKDKATAVKENIKDMPTQTAYAVHSAKEKAKSSVSDFKRGVVQE
                     KENRQSGRLKKQQLHRQNIADKRMELQKAQESRQAGKKTDGSATSGATRPHERPATTS
                     KVNVEKQQEVKRPAIASTVKNDEPIKKNVVKERPLSSGDTSAKTVNQETTRQNYKTDR
                     VTKTTVQKHTQQNTEKNKRTVFKKGQKKK"
     gene            29899..30906
                     /locus_tag="KPG09_000769"
     CDS             29899..30906
                     /locus_tag="KPG09_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBG0965395.1"
                     /translation="MKLRHIAIVGSLFSILFSLVLFFGVLISAEDDDGGNGYSSNYVG
                     MNLSAEVLKHQPMVEKYAKENGISEYVNVLLVIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGISDINVVIQSYNYGGGYVGYVAGNGKKHMFNLA
                     ENFARDKSGGQKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLVVNQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYNKAGVSLPRTAQNQYDATQHIPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNTQMYHAGDPIGYADLNNAYWQQYLICAGRIK
                     Q"
     gene            30922..31815
                     /locus_tag="KPG09_000770"
     CDS             30922..31815
                     /locus_tag="KPG09_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG0965396.1"
                     /translation="MFKKKEKPVKETKVRTMKVGTHKKSVIALWVVLIASVSFGVYKN
                     FTAIDMHTVHETETIQLRLNDTNGVENFVKNFCKSYYTWDNSKEAIEARTQAISNYLT
                     KELQDLNLDTIRTDIPTSSTVTNVLIWDIKQSGTNDFIATYEVDQQIKEGEQTSNVKA
                     TYTVKVHVDKDGDMVIVQNPTIAPAIEKSDYEPKAQEADASVDADTVNDATAFLETFF
                     KLYPTATEKELAYYVAGNVLEPIGRDYLYSELVNPMFTDDRDNVKVRVAVKFIDNQTK
                     AMQVSQFELVLHKDSNWKIIM"
     gene            32028..32885
                     /locus_tag="KPG09_000771"
     CDS             32028..32885
                     /locus_tag="KPG09_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0965397.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERNSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGIHYLSHGKSPKIKKNYTMEDLLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPAWSHRFMGLIQLGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVASLFNADLSCLENG
                     AIQCPVILITAKGDPLFRFDYTNKVFDLIHVPKKELLTLELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            32963..33544
                     /locus_tag="KPG09_000772"
     CDS             32963..33544
                     /locus_tag="KPG09_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0965398.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFSLYGLKKVTVDDLAAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTVKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEGLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMVQNLLVNGIIQQVVAE"
     gene            33648..34493
                     /locus_tag="KPG09_000773"
     CDS             33648..34493
                     /locus_tag="KPG09_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0965399.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KILTGLNTSYKGSVKVAGTEIREHTNRFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     DKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNNRLMKDIILAEKKRGKTIIITTHNMYDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIHYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            34490..35173
                     /locus_tag="KPG09_000774"
     CDS             34490..35173
                     /locus_tag="KPG09_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0965400.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALTGHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKVSEYILSKTIALIAISTI
                     IAVILGFVSGSNHLLGIAVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIVCFV
                     PPIVGLFVKLPDIFRFFPFTACMNLITGKSILLSFDMVLVIATLIILYIVARHTVKHM
                     WRSLGGVKL"
     gene            35170..35868
                     /locus_tag="KPG09_000775"
     CDS             35170..35868
                     /locus_tag="KPG09_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0965401.1"
                     /translation="MKRILSSTIQVFKQIKSDPMMFAACFTPFVMGALIKFGIPFLDG
                     ITDFSLQTYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLMVALVW
                     YYFLIKRLFRKLAG"
     gene            35880..36791
                     /locus_tag="KPG09_000776"
     CDS             35880..36791
                     /locus_tag="KPG09_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG0965402.1"
                     /translation="MEKGRLLQNENINKAIQVSKITLIINLGLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVLSTIVVMVGIKISEKQPDKEHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSGQYNTLSVSSGIALTAAVLSIVIKEWMYWYTRSAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASIVICGCILKAALDIFKESINKMVDHS
                     CDNATETKIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELPYSP"
     gene            36844..36984
                     /locus_tag="KPG09_000777"
     CDS             36844..36984
                     /locus_tag="KPG09_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965403.1"
                     /translation="MEQYWWIIIVMVLIVVGLLKIVIMKKYNQRRNSNNRSKNSEDDK
                     MY"
     gene            37099..37290
                     /locus_tag="KPG09_000778"
     CDS             37099..37290
                     /locus_tag="KPG09_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HBG0965404.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            37314..37544
                     /locus_tag="KPG09_000779"
     CDS             37314..37544
                     /locus_tag="KPG09_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965405.1"
                     /translation="MIGLKRRDMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            37810..38100
                     /locus_tag="KPG09_000780"
     CDS             37810..38100
                     /locus_tag="KPG09_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965406.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELRRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKNVTMY"
     gene            38100..38234
                     /locus_tag="KPG09_000781"
     CDS             38100..38234
                     /locus_tag="KPG09_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0965407.1"
                     /translation="MYYFLEMSDTEIADIMNYTREAVFKHRHRALETMKEILNEKTRQ
                     "
     gene            38215..39396
                     /locus_tag="KPG09_000782"
     CDS             38215..39396
                     /locus_tag="KPG09_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG0965408.1"
                     /translation="MKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYSWKLVP
                     TDKTPAGKRDDISLREKEKQIKKDLDDGIDTVGGKMTVCQLYEKKNNQRKNIKRNTEL
                     GRQYLVNALENDPLGMRAIDTVKQSDAKEWAIRMNGKGYSYKTIDNYKRSLKASFYMA
                     IQDDCIRKNPFNSKLSDVLEDDTEAKVILTPEQEEKLLAFMETDNVYSKYRDEVILLL
                     ETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISKAKTKNGKRELPLTERAY
                     QALKRILKNRGKAQPLVVGGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHKDKL
                     PNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELERLA
                     S"
     gene            complement(39736..40548)
                     /locus_tag="KPG09_000783"
     CDS             complement(39736..40548)
                     /locus_tag="KPG09_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0965409.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRIL"
     gene            complement(40624..41859)
                     /locus_tag="KPG09_000784"
     CDS             complement(40624..41859)
                     /locus_tag="KPG09_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBG0965410.1"
                     /translation="MGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITSGVTGMF
                     CHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLRGSLASLLEVESGSNDPIAY
                     MLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFYTIF
                     ITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIMLFF
                     MLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVGLRG
                     AASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTPVLK
                     TFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGSTII
                     KTGDILVLSGDRIEELIQL"
     gene            complement(42007..>42220)
                     /locus_tag="KPG09_000785"
     CDS             complement(42007..>42220)
                     /locus_tag="KPG09_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0965411.1"
                     /translation="NWHGRQIVKVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCN
                     ETHDRDINASINILKEGLRLITIQNK"
BASE COUNT        13710 a         7479 c         7364 g        13667 t
ORIGIN      
        1 ttaaagcaca gcttattata agatctaata tgggtcgaaa catatgattt aataaacttt
       61 tatttttcaa ttttatatcc tactccccat acagtatgaa ttattttttc tccatctgta
      121 tatttttcaa ttttatccct tattctgctc atatgaacca ttacagtatt attagattgg
      181 taatatttct ctttccatac cttttcaaat atttcttctg tactatatac tctgccttta
      241 ttatttgcta gtaaatataa tatatcaaat tctcttgatg tcaaaattat ttcatttcca
      301 gccactgtta cactatgttt ttccttatct ataattacta ctccaacctt tatgacatct
      361 ttatcttcat tattagcacc actaaaataa tatgctcttc taatcagtga tttcactctc
      421 acagtaagtt caagtgggtt aaatggtttt gtcaaataat catctgcacc agtcattatc
      481 ccttgtattt tatccatatc ttctgatttt gcacttaaca taagtattgg tatattcaaa
      541 ctttctctaa cttttctaca aacttctatc ccatccattt taggcatcat tatatccaat
      601 atcattaaat gaatattttc cttttttaaa atatctagag cctcttttcc atcttcagct
      661 ttaattacat tatatccact atttaaaaga tatatctcta taaggtctct tatttccttt
      721 tcatcatcca caacaagaac tgtattattc atttttacac atcctaactt aatctttgta
      781 tatagcttta ttcatataaa attatactat attttagtta gtgtaatcta taaatttatt
      841 catttctaaa tttccaatat aatacaaatt atttacacat ttatattatt tattttgtaa
      901 atattcttct aaagttaatc cttttgaata aatttcctcg gcagcatcct taccaacata
      961 tctaatatgc catggttcat atgctgtacc tgttatatct tctttacctt caggatatct
     1021 tattataaat ccaaacttgt gagcattttc tgccaaccat ttagcttctt ttgtacttcc
     1081 aacaaaccat ctttctggat ttgttaaatc aatacaaagt ccagtttggt gctcactgta
     1141 accaggcttt gctacatatg catctgcctt ttctcgtccc tgagatttta cccttcttgt
     1201 ataagtatcc agttgagtat cataagatct gtatgctgaa gaccctaaaa actgtattcc
     1261 ttctgaatta gcttgcttaa ctaaatcttc aactgccttt gcaggttctc ctgccatttg
     1321 tttttcttcc tctgttgctt cttcaacaaa aggtatgttt accttagtaa tattttcagg
     1381 agtatagttt ttgctaattc catttgtctt gtttaccaat aatatatttg atatactatt
     1441 tgagtgattt ttattttgag aatttgcctc aacatcttgt tctgaatcat gttgagcagc
     1501 tactattgga tctttatctt tcttaagtaa tctatcttta gatataaata cacttataaa
     1561 tactactaaa ataatggctc ctattaattt aaattttttc ataattaaat cttctccttt
     1621 ttgttatgtt tgtctataat gttaatttta gagtataaga cttaagtatt ttataagtaa
     1681 atcttaaata aatcttaaat ttaatatttt taaaggatgg tgataaaatg tctatacaaa
     1741 aacttctaag taaagctcgt aaggctatac aagattttga tatgatacaa gaaaatgata
     1801 aaattgcggt tggactttct ggtggaaaag atagtttaac tctacttcat atacttaaaa
     1861 gttatcaaag attttctcca caaaactttg aattgatagc cataacatta aatactggag
     1921 gtgttgataa ttctccacta gataaattgt gtaaagaaat aaatgttcct ttttatgaat
     1981 ttcaaactga cataaaagaa attgtatttg atataagaca agaaaaaaat ccttgtgcat
     2041 tatgtgctaa tttaagaaga ggtgctttga acgataatgc taaaaaactt ggatgtaata
     2101 aagtagctct tggtcatcat aaagatgatg caattgagac atttttaatg tctatgtttt
     2161 atgagggtag agtaaattgt ttttccccaa agacatacct agatagacag gatttaacag
     2221 ttataagacc tatgatttac atagaagagt atatgactaa gaaaatttca aaagattcta
     2281 actatcctat tatcacaaat ccctgccctg caaatggtca tactaggaga gagtacataa
     2341 aaaatcttat tgctaattta aataaagaga tgcctgattt taaaagaaat gtatttggcg
     2401 ctttaaataa ttctgagaaa ttgtttatat gggataaaga gaagataaaa aattttaaat
     2461 aataattaaa atttttaaat aaaaaagcaa gattgtatta attataaata atctttatat
     2521 aatctacaca atcttacttt tttatttatt atatgttatt atctttaatc tatattttaa
     2581 tagttcttaa tagtatttat tttgtctaat acattaatta taacttaaat attttatata
     2641 ttaatttaat atattaattc attcaatcta ctggtttaat ccatttttac agtatataca
     2701 tctacatatt ataaaaagca aaattatatt ttctaaatat tttatgttag attataatat
     2761 taaattctat tatctctggt atattaaaaa atctaaatgg taactttgta cttccaagac
     2821 ccgaactcac atataaatga tttccattta tatcataaaa ccctttagta tatttcctcc
     2881 catatggagg agtaactaat gctcccttaa taggtaatct tatttgacca ccatgactat
     2941 gcccagatag aactaaattc attgtatctt tactaagcat atctactaaa tctggttcat
     3001 gtgctaacaa taaattaaaa tttttattat ctatctgact ctccagatat tttatattag
     3061 gatttccatt taatatttca tcaacaccta atatattaat atatttatca tcttttaatt
     3121 ttattttttt attttcattt actaaaagat tgaagttgga atttttcata atctgcctat
     3181 aatacctagg aagtttatac atataatcat gattgccaaa tactgaaaat tttcccaacc
     3241 tagcattcat acttgataaa atagttgtaa tttcatctac acttggattt ttattggagt
     3301 aatcaattaa atcaccagta aatactatta tatctggatt caaagtgtta atcttattag
     3361 ctactttttt gagattctta gttgaataaa aactacctat ttgagtatct gaaatttgaa
     3421 ctacttttac actttcagat ttactctcaa ttgtatcatt ttttatcaat gctccatttt
     3481 cacttatatt atattttttc acaattaata gactcggctc tatcgcaact gagtatataa
     3541 aaatcaaaat aaatattaag ataaaaaatt ttaaaaaata cttaattttt gagttcaaaa
     3601 tatctaccta cttatttatt tttttaaact atttgcatta cagactcaac tttgttcatt
     3661 tttctgtcat gcatttttgg cttatttgct gaaaaccatt tatatgttgc tgtaagacct
     3721 tcttttagta acacatttgg tatataaagc ccatgttcaa taagcttatt tatatcaaga
     3781 ttaaagtcta tatttctaaa tgggaaatac gttctttctc taaactctac tttttccata
     3841 tctacatatt ttattatagg ttctttgcca ataatttctc cacatgtata tataaggtca
     3901 tcccaagaaa ttaactgtgg atttgtcaca ttataggcct ctcttacatg aggatttttc
     3961 attatgcttt ccaatacctt taccaaatcc ccaatatata taaattgatt actaacttgt
     4021 tttccttttg gtactggtat cttttcattt ttttctattt tttcaaagaa ataatactct
     4081 ctatataaat tattattttc accatatatg taagttggtc taataattat atatggtata
     4141 gggctattta taataaaatc ttcagcctct tttttattta acccatattt tccccagttt
     4201 tcattttcac ctttctcatt ttcttctttt atatttcttc cactatcttt atatactgct
     4261 cctgctgaca atactatata cttttttaag ttatccatgc ttataaaatc aatcagatta
     4321 cttacatctt cttttgtata tgctgtcata tcatatatgt aatcatattt tcttcctgtg
     4381 ataatgtttt ccatatcttt tcgaactttt ctatcacaaa ttagatgttc tttaaaacca
     4441 ttataatcta tttccttttt accatttgta agtatatcaa tttgataccc acatttaatc
     4501 aagcgcttag caagtgcaga acctatgaag tcgcttccac ccataattaa aatagatttc
     4561 attttaagtg catcctcctc ttttttaaat gacttcctta actataatat catattttta
     4621 aatatgcgac accattttgt aatatttttg tctataaaag atatttataa caaaatatct
     4681 tttatagact ctacattttc gacaatataa gtaggttcac tttcactaag ctcttcaaaa
     4741 gaaccatacc catacaatac tccaatagaa tctataccta tttttttagc tccaattatg
     4801 tcatactttc tatctccaac cataataact ttatctttat cttttacatt acataggtct
     4861 agtacatatt gtataacttc atttttattc actcttgtac catcaagatt gctacctgct
     4921 atatatttaa aatatctatc tatatcaaaa tatctaagta tagtttctgc aaaaactgtt
     4981 ggcttagaag ttgctactaa taatatttta ccatttttat agagcatttc taatatctct
     5041 ttcatattct cataaatttt attttcaaat atccctttat cagcaaaata ttctctatac
     5101 ttttcaacag cttctttagc ttttttatct tcaaatttat aatactcctt gaaagtatca
     5161 tgtaatggtg gtcctataaa ttggtctaag ttttccaaat cttcttttat gccaaagcta
     5221 tttaaagaat actgaataga ctttgttatt ccttctttag ggtctgtaag tgttccatct
     5281 aaatcgaata atactatttc ataatttttt ttcatatttc agtcctccat atttgtaaaa
     5341 agtaatatct tacatattaa gtttatctat tttaaaaagc tatagtaata gaaatttcta
     5401 atactatagc ttaagtaccc aattaaatct tatatattta ttttaaaaat aaatttaaac
     5461 agctctattt aactgttata gctgaaactg gacatccttc ttcggcttct ttacataaat
     5521 cttgtaaatc tgctggtatt tctccttcaa ttgctactga atgcccttca tcgctcatac
     5581 taaaaacgtc tggacatgtt cctatacatg ctccacaagc tacacataca tccttatcaa
     5641 caaatgcttt catactaaaa tacctcccct aaaattatta ttttttaatt gttgtttcct
     5701 tagatattta tccaatatat accaatttaa acatatattt attattttat cactattgtt
     5761 tagtttatta tagtattttc gtatccatgt ctataaactt cactgctttt catccatgat
     5821 tttacagtat attgcaatct taatttttta tttaaaacaa gttcattatt attgtagtct
     5881 tttataatat cattacactc ctccattgtg aatattgtaa aatcaccttc tcctccaatc
     5941 tttattctgc ctttatttcc atgtaaatcg aaaatctttg ttgggtattc agttactttt
     6001 tctatacact tttctaggga tatcccaagt tccataattt tatttataac atttaaaaga
     6061 gaatagttta cgtctttcat gttttttata tgcatatcag tacttattag gtcaggctct
     6121 aaaccttgct ttattgcttt tttaaaaact ttaaaatcaa attggtccac tccatgacat
     6181 acatcgaaca atactcctct attctttgca tttattactg attttcttaa tgtgccagat
     6241 tctttaagga ttccatttgt actatttcca tatactggtg taaccacatc tccttttccc
     6301 aaaatattca ttacttcatc tatatatgat ggatactctc ctatatatac cactaagggt
     6361 aaatttaatt ttttagcaac ttgctttgat ttcttaatta tatctatacc actttcatta
     6421 gattgaaatt tactcgcttt tacttttata ccctttatct tttctatatt tttttctaca
     6481 attgactcaa ccttgtcaaa atttataaca tctaaagaag ttgattcatt caatatattt
     6541 agtccctcat ttgtaacatt taaaaaagaa tatacttctg ttttacactc atccatatac
     6601 ttttttttaa aatcatagta gtttgaggga ccagctgtac ctgcatcaaa tataactgta
     6661 gaaccttttt caattccaat tgaatcagaa tccatacctg taggtaattt tctcttataa
     6721 caatgggtgt gaacatctat aaatcctggg gaaatataca tattatcggc atttattatc
     6781 tcgaactctt tatttgtaag taatttttct tttcttattt tctctttaag gttcaatcca
     6841 atagcatata ttttcccatg taaaaccaag atatctcttt tttcaaattt gataccatct
     6901 gttggagata ttattgtccc atttcttata agtaaatttt tcatttattc cacatcctaa
     6961 ttacaatata atttctctaa tagcttttct tacataaaga aaataagcct aaaaataaaa
     7021 atattttaga cttattcact atattatact atattaatta tattaaatta aataaaatct
     7081 ttaatttact ttaagaagca attaagtatt tgttgctcgg cttcttcttc agtaagacct
     7141 aatgtcatca tctttagtat ttggtctcct gcaatctttc caatagctgc ttcatgtact
     7201 aaaagagcat ctggatgatt tgcagcaatt tcaggtatag cactaatttg agaatttccc
     7261 ataattattg aatcacattg aacatgtgca gaacaaaggg aatccccaat aacacaagga
     7321 ttaaactttt gcttagaatt atcttgccca actgtacgag aaactacttg agcagaagag
     7381 ttttcaccct ctaagataac cttcatatta gattctgcaa cttgattacc atgagttaat
     7441 aatttctcaa atacaactat tttagctcct ttacctaggt ttgcatccat ttctctttta
     7501 gttgaatcta cacccttaat ttgaattgtt tccatttcac aatatgaatc ctcatccata
     7561 ttgacaatag taactggatt taaaattctc tttccttctc catcaccttc accataatgt
     7621 ttttctacat attttaggcg agctccttta cgtaagaaaa atctatgtat accattatgt
     7681 tcactatcat cacaaccaca gttatgtata ccacacccag caattataac aacatctgca
     7741 ttttctccaa tttcaaaatc attatatacc atatcctgta caccagcctc tgttactatt
     7801 actggtatat gtacattttc tccttttgta tttggcttta ctataatatc tattccaggt
     7861 ttatcagttt taggaattat agttacattt tcagttgaat gtctctctac tccttgccca
     7921 tttttacgta tattgtatgc tcctaatgga gttccacgaa gttcagcaac tttttctaaa
     7981 agatttaaat ctattttgtt catttaattt gccttccttt ctaattaatt catgtctttc
     8041 attaattcac aacttgaatt aacttgacac atgatttcag gtaatatagc ctcttttgtt
     8101 ccatggcttt ttactgaacc atcttgtaga acaattattt catcagctaa ctctaatatt
     8161 ctttcttggt gagaaattat gattatagtc tgattagact cttcatgtat tttcttaaaa
     8221 ctttcattga gttttccaaa gctccaaagg tctatccctg cttcaggttc atcaaatata
     8281 gaaaccttta aatctctagc aaaaagagtt gctatctcaa ttcttttcat ttcccctcca
     8341 gaaagagagt tatcaacttc acgatttaaa tagtcttttg aacacaatcc aacatctgta
     8401 aggtattggc aacatacatc agtagacaat ggttttccat gagcaagtgt aagtaaattt
     8461 tcaactgtca taccttttat tcttggtggt tgttgaaatg cgtatcctat tccttttttt
     8521 gctctttctg taatactaag atttgttata tcttctccat ctaaaattat ctgaccactc
     8581 gtagcttttt caattcccat aataagctta gcaattgtag attttcctcc accatttgga
     8641 cctgtgataa caataagttt tcccctctca aatgaaaggc taacatcatt tattattcct
     8701 agttcctcat tgttagactc aacattaaag gaaagatttt ttatttcaag cataataccc
     8761 ctcttttcta taaaatatta cattaatatc cattctttta ttatatcagt aatttttata
     8821 tatcagcaac tctaagcaga taaaatgtat attaaataat aaaacaaagt tattgaaaag
     8881 taaaaaagat gagttttatg taaactcatc tcagaaattt agtatatcaa cttatatgta
     8941 tttaaaattt aaccttctat atactctaat attatttttt gattatcttt taacataatt
     9001 tcttcttttt tagattctat tccattcttt tttatcttta cctcagctac aaaggcatca
     9061 ttaccttcaa ccatttctaa tagttcatac gcttttttac gacctatttt agttgctata
     9121 tcttttagta ctgccatttt cttaaacatt ttatcacctc tactcaaaat ttacacttaa
     9181 tttttaatta tataattcta atcctagact agtttataat aattattgtc tctaattata
     9241 tcagatattg agtaaattta atactttgta aaattattta tcctctctga ctaaataatc
     9301 atttccatct tggtctttaa aagaaaacat ccttccatat ggcatctcca ttaattcccc
     9361 aacttcaacc tcgttatttg ccatttgttc ataagttcca ttaatatctc ttgtacttaa
     9421 aattattgat ggatgagata catttatctc tttgttttgc ttcatcatta gatttttatc
     9481 atacaacaca aatgttgtaa attcattttt acttggtgct acttccaccc atttcatatt
     9541 tggacccatt tccttttcat aagttactac aaagttcaat ttttctgtcc aaaacaactt
     9601 tgcatcctct tgattattta catagagagt tatttttcct attttattta tcataacaat
     9661 acccaccttt attattaaat aaatacctaa tattttttat atttaataat atacccatat
     9721 atattatttt ctatagatat atatttctaa tatttatcgc caaattatgt aattctgctc
     9781 aatatataat ttataatatc catactatta tagcctttat ttatgtaatt taataacctt
     9841 atatatcata cttataatta tgataataac tacataaaaa atcacttcta aatagagtta
     9901 tccatctaaa agtgatttca tatattctat gcaactttgt gttttagcca ctttgttcct
     9961 cttaatctta aacaatataa agtccctcta acaagccaat cagtatacat acctatccaa
    10021 attcctaaca ctccaaattc taatacaacc cccaatacgt aacctgtaaa aattctaaaa
    10081 atccacatac ctattactgc agtcatcatt gtatatctag tatcaccggc tccctttagc
    10141 cctgctgata atacaaagga tataggccat attaccatag ctaaacaatc acttcgaatt
    10201 aattgtgttg agatgtcaat tacactagca ttttgagtat acatacttga aatccagggt
    10261 gcaattggta taaatatagc tcctactata aataaacaaa ttgttgcaaa tttagttaaa
    10321 tataataaag tactcttagc tcctttaata tctccacgtc ctatatactg tcccaccaaa
    10381 gttgtagcag acaaagctaa agcattccca attacattta ttatggatgc aattgacatt
    10441 cctattgcat tggatgcaat tgctgatgtt cccatagtaa ctataaatac ttgaacaatc
    10501 aatttacctg cattaaaaag tagttgttca actccagctg gtatccctat attaaatata
    10561 gacttttgag tttcaacatc aatcttaaat ggaaatattt tacccataga tataaattca
    10621 tttcctttaa ataaagctct tataattact atacatccaa ttattctagc tatagcaata
    10681 gatatagctg cacctgttat gccataggat ggtgtttgca aatttattcc tagaggattt
    10741 aaatcaatac catatataag tatatatcct aatgttacat ttactatatt cataaacata
    10801 gtcacataca taggtttctt tacatctcca caccctctta aaataccatt tgctatttgt
    10861 tgtgtcgcaa taaatggata cgtaaataat gtaaactcta tgtataattt tgcatttgat
    10921 tttaccaatt cttctgctga accataaaat aaattaatta caccagttct aaaaacccac
    10981 atcaatattg ttattgtaaa ggataatatt actccagaca ctagagcctg cttaatagtt
    11041 tcattcacct tttcactatc tttttttcct atttgttgag ctacaacgac agtagctcca
    11101 acagataatg cagcaaatac agatataaat atattgttta ttgaatcaac cattccaact
    11161 gcagatacag cttcttcccc aatatgacct gccattattg tattaaatac tcctaaaagc
    11221 atgacaaacg tctgctctat cattatagga attgctaact ttataacatt tttccttata
    11281 aacatcccta ttcctctttt ccctctttat ataaatttaa tttttaatta gtttatcgaa
    11341 ctgtaaattg acaatgcaaa gtttagaatt gtattgttcg cattataata tactgctttc
    11401 aaataatata aatcttgttt aatatcattt gaacacagta tatctttata attatcttat
    11461 gtttattatt ttttatggat accacattta aaatatttat taatttaata taaatatttt
    11521 ccgttattat taaaagtagc tatatttcgt taatattgta tcactatcgt gtagcaataa
    11581 caataagtac attttatata ttttttataa tattatttaa tcatccaaac ttttaccact
    11641 ttttcaatat ataaaatatt acactttaga tgtaacaaaa tcgcatccat ttaaatattg
    11701 tattttatag actaatttta ggatgtgata ctttggttaa tttaaatgca aataatctaa
    11761 atgtaaatat ggtttatcct atttcttcca cagcacccat acgtttcaga agatatactg
    11821 tttcatattt agaaaacatg gaaaatattt atttaactat agcaacatac tttgatacac
    11881 ttggtttagg cacaatgaat atcgacaaag tttatggtca atgggcttgg tttactgata
    11941 atatatatga tttaaatcta tttgtttttg taggaaatta tccttatcaa attgctaaaa
    12001 acagatatga tacatttgta gatattttgc cagtagctat tacttctatt gtcaatggag
    12061 atagagtatt tttaatttca aatccttgtt tgttaaattc aaaaattaca gttagattta
    12121 tatctagtca tcctactctt aatagaacag ttgattatgg caatattaga gattttattt
    12181 tttaaaactg caacatgatg tgctattaaa aaatacccta aatttaatga tattattaaa
    12241 taactagaat atttttaatt ggcacatcat gcctagctat tataaattca taatattatt
    12301 ttacttaaga taagtactgt attttatcta cttgtatatt tttatttacc tagtttctca
    12361 gttctatatc ctatttcttt attagctagc ttataacctt cttgtatcat ttcttctctc
    12421 gttaatgcac tccctaataa attgtcaata ctcagttgct ctctatcact aaaatactta
    12481 tccaataatg attcctctat atcttcctga tatgccaaat ttccttcatg cattatatat
    12541 tttacatcga aaccttcttt atttaatact tttcccacca aaatacttct atgacatctt
    12601 ataggttctt gcatagctcc taataaaact atcctataac ccatttgaca accctttttt
    12661 aatctttcta ttccgtttaa aaaaatatct tcttttgcta ctttctcaaa atctgcataa
    12721 ccttcttgcg tatatacatc tttattattt cttttagctc caaattcttt tcccatgtat
    12781 atatatataa atccggaatt tctaagagat tcgttaaatg cctctttatt gtattgaata
    12841 ttatatttag aatatggagt ttctctaatg tcaactacac aatttatgtc atattttttt
    12901 atcatattaa taagcttatc atatggataa tttgaatgtc ctattgcgaa tatttccata
    12961 taaataccct cctattcttt gaaattagta tctatatatg tagatacccg atttacttaa
    13021 aattaaataa agtattactt taaataccca atatcttttt ttcttgatac tgtatatttt
    13081 tttactttat atccttaaaa aaatatacaa ctttatatat aaaacatcaa tatttatata
    13141 tattttgtat attaaataca attatttata aaatctttga taaaaattta taaatagagc
    13201 atctttagaa aactattaac agaaaaacac actaaaaatt tgattatccg ctaaaacact
    13261 gtatctatcg gcattatcaa ggagctaaac acatcaaaat actactaatt tactacgatt
    13321 gaatgagcct tgatacgtcc tatttcccag atatgcaaag atacggtaca gcacatcagt
    13381 attgaaacaa gaaaaattga atacttcata gactacggaa cgcacaatat cagcgttcct
    13441 tttctatttc cagccgttcc tagtggacgg ctattttact accaaaatga aaggagcatt
    13501 tagaattatg aaaaagatat taaaacgatt gtgtacaggc tttcttgcct ttgcaactgt
    13561 cgttaccgct ttaccgagtt caacggttca tgcgtctaat acacagtatt ggacggaaag
    13621 taaagagcgt gtcagtatcg tagaacaagt aatgaatgac ggttctatca gttctacctt
    13681 taatgaggga catttaaccg ttgagggtga ggacgcttat tgtatcgaca tcaacacagc
    13741 ctttaaaaac ggctataaga ccaaagctga cgcaagtacc cgtatgagtg ccgaccagat
    13801 agctgacgtc gccttatcta ttgagtatgt gaaacagtac acagacatcc acagcggaat
    13861 aagcagtaaa cacgcctacc tattaagaca gcttgttgtc tggcagagat taagtgttca
    13921 gcctgactgg caatgggata atgtgcgagc ttcctatgat gaaatcccaa aagccgtaca
    13981 agatgaagta ttcgcaggag caaaagcctt tgtgaaagaa aacaaaggac gttatgactg
    14041 tggcggatat atctactctg gcgagggtca agagttaggg caattctggg cgaagttagc
    14101 tgtcggaaat accaaacttc aaaagacttc caccaatgcc aatattacag acggcaacgg
    14161 gatttattca atcgctggtg caacatacgg tgtgtattct gataaagact gcacgaaaca
    14221 gcttaccacc cttacgactg atactagcgg aaatacagaa gccgttgaag taagagccac
    14281 tatagtatat atcaaggaac tttctgcacc agcaggattt aaaattgata aaacagttta
    14341 ctctttatct gtggaagttg gaaaaacagc gactttaaaa gtatctgata caccaaaagt
    14401 aacagatacc ttgattgagc tattcaagat tgacatggaa acacagaaat ccaatccgca
    14461 gggaaacgct tctttagagg gagcagagtt tacatggaac ttctacgctg gatattacaa
    14521 caaaaacaat cttccagcac agcctacaag aacatgggta acaaagacta tcgctgaaaa
    14581 agacagcgac ggtgctatcc attacattac tcgtttagct gacaaatata aagtatcttg
    14641 ggacagcttc tatacgcagg acggtaaaaa cgtacttcca ttaggaacac taactgttga
    14701 ggaaacaaaa agtccgtcgg gttacttgtt agagggtaca tatatgcagg ctgacggtag
    14761 cgaagaacag ataaaaggaa tgtatctgac acagattaca gaggacggcg accttgccgt
    14821 attatctgga agcaatcaat accatgtttc tgataaagtc attcgtggcg gtgtgaaaat
    14881 ccagaaacga gatttagaaa ccaaagacac gaaagctcaa ggcggtgcga ctttgaaaga
    14941 taccgccttt gaaatcattt ccttaaatga gaatgccgtc ttagttgagg gcaagttata
    15001 caagaaaaat gaagtgttaa agacaatcca taccgacatt gagggtatcg cttctacttc
    15061 tgctgaccta ttaccttacg gaaaatacag attgtctgaa caaaaaccgc cagagggata
    15121 tttgactgac ggtgcaaagc cgattgattt tgaaatcaca gaaaatggaa aaatcgttga
    15181 tttgactgac gaagcccact ctatctataa ccagattaaa cgtggcgata ttgagggcgt
    15241 aaaaattggt gcaggttcgc acaagcgtct tgcagatgtt ccgtttagga tcacaagcaa
    15301 gacgacgggc gaaagtcatg ttgttgtaac tgatgataac ggacaattct ctactgcttc
    15361 tgattgggct tcccacaagc acaataccaa tgcaggaaaa acaagcgagg acggtgtgtg
    15421 gtttggtact tctgaaccag acgacagcaa aggtgcgttg ctttatgata cttacatcat
    15481 tgaagaatta cgttgtgaaa gcaacaaagg tttcaaactt attccaccgt ttgagattgt
    15541 catttccaga aataaggttg tggttgattt aggtacgctg actgatgaat acgaaaaaga
    15601 aatcactatc catactactg ctaccagcaa ggacggcgaa aagactattc ttgctgggaa
    15661 agacgtaact atcactgata cagttaaatt agacggtctt accaaaggga caaaatatca
    15721 gttgaaaggc tggcagatgt taaaagaaga aaatgcagag cttatcatca atgacaagcg
    15781 tattgagaat gactacatct ttacggctga cagcgaaact atggaagtta aaatcgagtt
    15841 tacctttgac gcttccaatc ttggcggtaa acagcttgta acttttgagg aattatacga
    15901 tttaagcaat ccagacgaac ctatcaaagt tgcggaacac aaggacattg aggacgacgg
    15961 acagacagtt actatcaagg aagtaccaga aacacctacc ccagaagaac cagagaagcc
    16021 aaccacacca gatacaccta cgaaaacaga cagtcccaaa acgggcgaca atacaaatat
    16081 ccttgccttt gcaattatga tgtttgtttc tgctggtggg ctggctggta catacttttt
    16141 caaacaccgt aagatgaaga aatcataagg cggtagctgt atgagaaaag aaaaatcacg
    16201 ctctccgcca tagcttagat aggcagacta tacctattct ggcttgtctg ccgattacct
    16261 gcaagaacac ggatagtcaa gggtgcgtta gcatttattt accctttact atctgggggc
    16321 ttgctggcac tttcctaaat gccagaatac acacatcaat caaacttaaa gtaagaaacg
    16381 aggtaaaata atatggaatt aaaatatatc gtgccgaatg tggcacagac ttttggaaac
    16441 ttggaatttg caggggaaaa caaagtagac cagcaacgta tcaatgggcg ttacactctc
    16501 gtatcaagaa gttacaactt gtattcggat attcagagag ccgacgacat tatcgttgta
    16561 ttgcctgcaa aagctggcga gaagcatttt aagccagagc agaaagtaaa actgattaac
    16621 ccacgcatta ccgcagaggg ttacaacgtc aaaggacgtg gatttacaaa ctacatttta
    16681 atggctgatg atatgttacc agcagaaagc aaataagggg gtatttgatt atgagattaa
    16741 caaatggaat tgtattagat aacgaaaaaa cttttggagt attgaaattc tctgcacttc
    16801 gacatgaggt gcgtgtagaa aatgaggacg gaacgacaag tgatgaaatt aagcgtcgta
    16861 cttatgattt gaaatgtagt gtgcaggaac gcatgataca agtgtcaatc cccgctgatg
    16921 ttcccgtaaa ggactttcct tataatgcgg aagtggagtt aatcaatccc gtagctgata
    16981 cggtggcaaa tgccaacttt agagggacta cggttgattg gtacatcaaa gctgatgata
    17041 ttgtattgaa gaacaaaggc ggtcaagctg gaaatccgca gaacaacgct ccgcagggac
    17101 agcagaaaaa gtaagctgac aaactagttt ttcattgtag gagatataaa caactgttat
    17161 aatatggatg tggaaacagt taatttagac ttataaagga gaaatgtgta tgctagataa
    17221 aatacctaat gcagaggaaa tgactgcttt ggtcggacaa tcattatata gtgtatggaa
    17281 tgaattgtgt gccttgattg atgaaaagta tgaaatggaa tgtctatgga ataaaggtgg
    17341 caaagcatgg aattatgaat ataagtatcg tcgaggtggc aaaacattat gtgccttata
    17401 tgctagagaa aattgtgtgg gatttatgat tatcttaggt aaagatgaac ggttaaaatt
    17461 cgatacatat agagataatt attcaaaaga agttcagaga atttatgatg aaacaaaaac
    17521 ttaccatgac ggaaaatggc tgatgtttga gccaacagat acaactttgt ttgacgattt
    17581 tgttaaactt ttgaggataa aaagaaaacc aaatagaaag tagtttatta ccacaccatt
    17641 agcaaatgga tagtttctta taatagcgga tatatgaaaa tgctgtcatg tggacataaa
    17701 gaaaattgga agttgtggag gtagcaaaaa tgaaattaaa aaaccctatg ttggtagtaa
    17761 ctgatataga taaatcagtt gagttttata aaaaggtttt tggactttat gtcattatgg
    17821 attttggggc aaataaaact ttaacaggtg gattagcctt acagacaagt gaaacataca
    17881 aagagtttat cggtacaagt gatatttctt ttggtggtaa caattttgaa gtctattttg
    17941 aagaagaaga ttttgataaa tttgctgata ggctgaaaga atatgatatt gaatatgtcc
    18001 accctattat agaacattca tggggacagc gtgtagtccg cttttatgac ccagataaac
    18061 atattatcga agttggagaa aacatgaaga tagtgtgcaa gcgtttctta aatagtggaa
    18121 tgacaccaga acaagttgca gaacgtatgg atgtacctat gaaatttata aatgcttgta
    18181 tgcgataaat tgaaagttag gagactaata ctatgaaagg atttgaacgc aaaaatcaat
    18241 tattttctct ttgcggattg aattgcggac tatgccctat gcttttggga aattactgtg
    18301 gcggttgtgg caatggtaat caatcatgca gaattgcaaa atgtagtctt gaacatggac
    18361 agattgaata ttgctatgaa tgcaaacaat atccatgcga aaaatatcaa cacattgatg
    18421 attatgattc tttcatcacg cataaacggc gaaaagcaga tttagaaaga gcaaaaaaca
    18481 taggcattga gcaatataat cttgaacagc aggaaaaaac acaaattctt tcttacttac
    18541 tttctaacta taatgacggg cgtagaaaga ctttcttttg tgtggcagtc aatttattag
    18601 aactttcaga attacaagaa gcaatgaaac aaatacaatc caatgacgaa ttgaccttac
    18661 taccatttaa ggcacaatgc ttgtatgtgg tggaggtgtt tcagaagatt gctgaaagga
    18721 gaaatataaa actgaaactt ttcaagaaaa agtgattttc aaatttgaaa ttgtaacaaa
    18781 gattatttat atgacgaatg ggggaatacc tttggaaaca gtagagttaa gatttcaata
    18841 tactcaaagt gaatatataa aagcagaaag gcaatattta atttctagta aaactataca
    18901 taaatatgat attgctttag tcgctgtatt tttgttgctg tcagtcgttt atatgctgtt
    18961 ctcctctttt agcgtattta gtattttaat atttggattg ataattgttg taactgcatt
    19021 gggaagctat ttatatatcc taatgcctat actgaaattc aaacaaactg caaaatatca
    19081 tgaggaatat acgctggtat tttctaagga aaacattaaa tttaaaacac aaagcattga
    19141 ctcagaagtg aagtgggaca tttactctgc actatgggaa agccatgatt tttattactt
    19201 aattcaagca ccacgattat atacgttaat tcccaaaaga atttttaaag acctaaacga
    19261 aaaacagcta tttgaagaaa ttgcacaatc aagagttaaa acaacaaagc atatataatg
    19321 aacataccct acaatgtaac aacttcatat tcatacacac aaagcggtta gtcttttaag
    19381 attgactgct tttttcatac ttaaattaag attggagtga ttgatttatg cgaatgattt
    19441 tgaataaagg acaccgtatc agagccagag acaagaacct tgtctaccac ttttccatag
    19501 ggacgcttct gtttgtattt gtggcggttc tcctgctact gaatatcaaa cagctcatgc
    19561 gtaccgactg ggaacatttc agcttattgg acaatggctt gacgctttcc ccctacaact
    19621 tcataaccat attgatagcg actggtattt gtgcattggt cgcttttctg tattaccgct
    19681 tctgttatga cagtttcaaa aagctcctgc accgtcaaaa gctggcacga atgatactgg
    19741 aaaacaagtg gtatgaagca gataccgtac aagacagcgg tttttttact gacctgcaaa
    19801 gcagatcaag ggaaaaaatc gtctggtttc caaagattta ttatcaaatg gaaaacggat
    19861 tgcttcatat tcactgtgaa atcacattag gaaaatatca agaccagctt ttgcgattag
    19921 aggataagct ggaaagcggt ttgtactgtg agctgaccga caagaccttg catgacggct
    19981 atattgagta cacgctttta tatgacatga tagcgaaccg tatttccatt gacgaagtga
    20041 aagcagaaaa cggctgtctt aggctgatga aaaatcttgt ctgggaatat gacgtactcc
    20101 ctcacgctct gatagctggt gggactggtg gcggtaagac ctactttctg ctgacgctta
    20161 ttgaagctct gctacatacg aatgctgtcc tttacatctt agacccaaag aacgctgacc
    20221 ttgcagactt aggtacagtt atgggaaatg tgtatcatac caaagaagat atgattgatt
    20281 gtgtcaatgc cttttatgag ggcatggtac aaagaagcga agaaatgaag caacacccag
    20341 actataaaac gggcgaaaac tacgcctatc ttggtttacc gccctgcttt cttatctttg
    20401 atgaatatgt ggcgtttttt gaaatgctgg ggacaaagga aagcgtaagt ttacttagcc
    20461 agttaaagaa aatcgttatg ttgggacgac aagctggata tttccttatt gttgcctgcc
    20521 agcgtccaga cgcaaaatat ttctcggacg gtatcagaga taatttcaat ttccgtgttg
    20581 gattggggcg tatcagcgaa ttgggttacg gtatgctctt tggttctgat gtgaaaaaac
    20641 agtttttcca gaagcgtatc aaggggcgtg gctattgtga tgtgggaaca agtgtcataa
    20701 gtgagtttta cacgccttta gtcccgaaag gacatgattt tttgcagacc ataggctctc
    20761 ttgcattcgt aaggcaggac gggacggcga cgtgcgaagc gaaaggcgac ggcacggact
    20821 agccgttgct ggtgtggcgt tagccacgcc agcagatata agcccctcat tatctaacag
    20881 aggggcacaa ttacgctgga aactggttca aaaaccttga aaagtccgct aggaataagg
    20941 attttcttag tttttagcta gttgtgataa tcacttgaaa atatcacaaa atcacaacaa
    21001 tatcacgatt gggggtatga caactgaatg atactgattt tctgaacacc ttgaaagcaa
    21061 aacgcagtga ttatggcgta tcgcagacaa ggcttgcaat catggctggt atcagccgtg
    21121 aacatctaag ccgtatcgaa gctgggaagg tagcactgac cgaggatatg aagcacaaac
    21181 tactggaagc ggtggaaaaa ttcaatcccg acgctcccat gttcctgctc tttgattatg
    21241 tgcgtatccg atttcccacg ctggatatac agcacgttat caaggacatt ctaaagctga
    21301 atatagatta tatgcttcac gaagatttcg ggcattacaa gtacaccgag cattactata
    21361 tcggggacgt gttcgtttac acttcacaag acaaggaaaa aggtgtcctg ctggaactga
    21421 aaggaaaagg ctgtcgtcag tttgaaagct atctgctggc acaggaaaga agctggtatg
    21481 acttcctcat ggacgcatta gttgatggcg gtgtgatgaa gcgtatcgac ctagctatca
    21541 acgacagaac gggcttactg gatattccag aactgataga gaaatgcaag aacgaggaat
    21601 gtatttctaa attccgtagc ttcaagaact atggttctgg ggagctggta aagcacaacg
    21661 aagccaacaa ggacggcatg ggacatacgc tttacattgg ttcatttacc agtgaagtgt
    21721 acttctgtgt ctatgaaaag aattatgaac aatcctcaaa attagggata ccgattgagg
    21781 aagtccccat taaaaaccga tttgagatac gtttgaaaaa tgagcacgcc tactatgcca
    21841 ttagagagct gctgacccac tacgacgcag agctgacggc attttctatt atcaaccagt
    21901 atatccgctt tgccgataag gaagccaaca agcgaaaaag cgactggaaa accaacgccc
    21961 gctggtcttg gttcattggc gagggacgac cgcccgtgaa gctgacgaca aagccagagc
    22021 cttacactct gcaaaggacg ctgaactggt tacaaagaca ggttgcaccg accttgaaaa
    22081 tgctgaaaca gatagacgct ggcaatcaga tggactatct caagaccatt gaacagcagg
    22141 caaagctgac agataagcac aagcagataa tcagacagca gacggcttcg gtggacgaac
    22201 tgatagaaaa agaaaaggag tgattgatga tgtgggaatt gattaaggac ttactgctgg
    22261 tatcacttgg catgggtatc ggcgtggttc tgatgtgtat tatgagtgtc ggaaaggaag
    22321 ctgaccgaca catgattgaa ttaaaagaaa gcgaggaaaa ataaatgaat ttcgggcaaa
    22381 atttatatac atggttttta agcaacgccc agagcttagt gcttatggcg attgtggtta
    22441 ttggtatcta cttagggttt aaacgtgagt tttcaaaact tatcggcttc ttggtggttg
    22501 ccttagttgc tgttggattg gtattcaatg caggcggtgt caaagacgta ctcttagaac
    22561 tattcaataa gattatcggc gcatagccgt actttttcat tatggtttgc ggatacgcaa
    22621 acataccttt cccactttgg gagtgggcta aggggaaagg gggtaacgga atggtacaca
    22681 gataaaaata cagcataatg aatttcattg cttggctcaa acatagttga tataataaag
    22741 atatgaaaat aataaacgaa agtttttttg ataaggagtg atattgtgaa attgtgtatt
    22801 gttttatctc catataaggc ttaatggaat agacttgtat ggagtgaaat gttatccaga
    22861 gagccttaca ttttatcaaa tttcaaaatg taaaggagaa tgggcatgga atataaatat
    22921 acaaaaattg atttgaataa atggaataga ggtaaattgt ttcaaagcta cattgataat
    22981 atgcgtattg ttatgagctt aaccgtagat attgatgtaa caaagtttat tgaattttca
    23041 aagaagcatg atttaaaatt ttatccagct atgatatggg ctgtttcaaa ggttgttaat
    23101 gaacatgatg agttcaaata tagttgggat aataatggga atttaattaa gtgggattat
    23161 atttctccgt cctatactga atttcataaa gaagatgaaa atttcacaaa gatagttaca
    23221 gatttttctg atagcatatt tgaatttcat aaacgtttta tattagatag ggagaagtat
    23281 aaaacagaac gtgcatttgt ggaaaatcaa tctttgaact tttttgatgt atcttgttta
    23341 ccgtgggtaa aatataatca ttttgatgtt catgtttttg acgagggaaa atttcttgca
    23401 ccagttatta cttggggcaa atatgaatta gagcaagaaa gatatataat gcctcttaca
    23461 atgaatatac atcatgccgt agcagatgga ttccatttat ccagattttt taatgaagta
    23521 caagaattga taaactcaat ttaacagcta tatacaaagc agttagtcta tacgattgac
    23581 tgcttttttt catacctaaa tttaagattg gagtgattgc ttgaaagata tattttcgga
    23641 tatgcaggca agtatcggct gtacctacat ttcagattta ccacatcata aacgtatggt
    23701 gtggcaggaa atgaagcgac tgaaccttgc cgattatccg cagaaacaat tagaagattt
    23761 tgcaggctat gtctttggtg tcccttacac tagcatagct gaagcatgaa agaaaggacg
    23821 tgaaaaccta tgaatgaaat gcgtgtctgt atcgaaagcc gactacacac ttctgaagaa
    23881 atccgttccg catggttcac acttcccatt gacgaggaag aactggaaga acagataggc
    23941 gttcctatcg acagtgaaga ttaccatatc gttgaaaagg aactgccctt tgctgatgaa
    24001 gtcggcgaag atgcgaccat tgaaaaactg aatgaccttt accatacctt tgaaagtctg
    24061 cctgccgatt tacaagagga ctacggggag ctgatgtgtt actttaccag ccttgacgaa
    24121 ctgcaccagc accgccatga tgtgatacat tattcatggt gtaaaaacat ggctgatgtg
    24181 gcaaggcatt tgttggacaa tgaccctgct ttttctgcca tcgaggaacg cctgacccgt
    24241 tactttgatt atgaagccta cggacagtat cttgatgata acggagaatt tatagaaaca
    24301 gaccacggta tctatgaact gccgtagaag tgaggtgttt ctatggtaga ggatatgcgt
    24361 gtatatatcg ctaatcttgg gaaatacaat gaggggtatc tggtcggcga ctggttctct
    24421 tttccgattg acgaagaaga tgttgccgag cgtatcggct tgaatgaacg gtacgaagaa
    24481 tacgccgtac atgatacgga aaactttccc attgagattg gcgaatatat ctcgattgaa
    24541 gagctcaatg aaatgtatga aatgatatgt gagcttcccg actacatcac ggacagtttg
    24601 gacgaatttg tatcaaatta tggaagtttg gaggaactgt acgaccatag ggacgatatg
    24661 attttatacg ctggctgtga ggatatgacc gacgttgcct attacttcat tgatgaattg
    24721 caggcacttg gcgatattcc ccctagtttg cagaactata ttgactatga agcatacggc
    24781 agggatttat cctttgaggg aacttttatt gaaacaagat acggtatgtg tgagataccg
    24841 tggtaagaag ctggaagatc agcgacaagc attgttgctt ctgacagctt tttctattgg
    24901 ggacagtctg aaaaacggct gtccttttct attatacgaa aggagttgaa acaaaaacgt
    24961 gaaaaagatt aaaagctaca cgggtatctg gaacgtggaa aaagtcttgt atgcaatcaa
    25021 tgactttaac ctgccctttc ctgttacctt tacacagatt acatggtttg tgataaccga
    25081 gtttatcatc attctgtttg gggacttacc gcccttatcc atgattgagg gggcattttt
    25141 aaaatacttc ggtattcccg tagctcttac atggttcatg tcgcaaaaga cctttgacgg
    25201 caagaagccg tacagctttt tgaaatcaca gataaccttt gccctacgac caaagattac
    25261 ttatgcagga aaagccgtga aactggataa gcaggtacta catgaagcta ttacggcagt
    25321 aaggagtgtg agctatgttc ccgattaaat atatcgacaa caaccttgtc tggaacaagg
    25381 acaatgaggt gttcgcctac tatgagctga taccttacaa ttacagtttt ctatccgctg
    25441 aacagaaatt cattgtgcat gacagcttcc gacagctaat cgcacaatcc cgtgagggca
    25501 agatacacgc tttgcagatt gccacagaaa gctcaagccg aagcatacaa gaacagtcaa
    25561 agaagctggt tacgggacga ctaaaagacg ttgcctatca gaagatagac gaacagaccg
    25621 aagcgttagt atctatgatt ggggacaatc aagtggacta ccgctttttt cttggcttca
    25681 agctcatggt aacagaccag agcatgaacc taaagagcgt gaagcaatcg gcgtttctca
    25741 cattcaagga gtttatccat gaagtgaacc acacgctgat gaatgacttt gtttccatgc
    25801 caaatgatga aatcaaccgc tacatgaaaa tggaaaagct gttggaaaat aaaataagcc
    25861 gacgcttcaa agtaagacgc ttagataaaa atgacttcgg ctatctgatt gaacacatct
    25921 acggcaggga cggtgtcgcc tatgaagatt atgagtatag cttaccgaag aagaaactga
    25981 aaaaggaaac gctgataacg cattatgact tgatacgtcc gacccgttgc cttgtggaag
    26041 aaagccagcg acatttacgc ttggaacatg aggacagcga aagctacgtt tcctacttta
    26101 ctgtcaatgc gattgtcggc gagcttgatt ttccgtccag tgaaatcttc tatttccagc
    26161 aacagcagtt tacttttccc gttgatacca gcatgaatgt agaaatcgtg gaaaaccgaa
    26221 aagcgttatc gacggttcgc aacaagaaaa aagagctgaa agatttagac aatcatgcat
    26281 accagtctgg cagtgaaacc agctcaaatg tggtagacgc attagacagt gtggacgagc
    26341 tggaaacgga tttagaccag tccagagaaa gtatgtataa gctctcttat gttatccgtg
    26401 tatctgctcc agaccttgac gagttgaaaa gacgctgtga tgaagtcaaa gacttctacg
    26461 acgatttgaa cgtcaagctg gtacgccctg ctggcgatat gctggggctt cactctgaat
    26521 ttcttcctgc cagcaagcga tacatcaacg attatgtgca gtatgtcaaa agtgattttc
    26581 tagctgggct gggctttggt gcgacacagc agttagggga aaacacgggt atctatatcg
    26641 gctattctgt cgatacggga cgcaatgttt atctacaacc gtcccttgcc agtcaaggcg
    26701 taaaagatac ggttactaat gctcttgctt ctgcctttgt agaaagtctt ggcggtggaa
    26761 aatcattctg taacaacctg cttgtctact actctgtgct gtttggcggt caagcggtaa
    26821 tcttagaccc aaaatccgag cgtggcaact ggaaagaaac gctcccagaa atcgcccatg
    26881 aaatcaatat cgtgaacctt accagcgaca gagaaaatgc agggcttctt gacccgttcg
    26941 tgattatgca gaatgtgaaa gacgctgaaa gtctggcaat cgacatactc acattcctta
    27001 cgggcatttc ttcaagggac ggcgagaagt tccccgtact tagaaaggca gtgcgtgccg
    27061 ttacccagag cgaccagcga ggattgctcc atgtgattga ggaattaaga aaagaagata
    27121 cacctatctc ttgcaatatt gccgaccata tcgacagctt cacggactac gactttgcac
    27181 atctgctgtt ctcggacggt acggtgcaaa atgcaatcag cttagacaac cagctcaata
    27241 tcattcaagt agccgactta gtgctacccg ataaggacac gacctttgaa ttactgtccg
    27301 tttccatgct gattgtgatt agtacctttg cgttagattt tatccactcg gatagaagtg
    27361 ttttcaagat tgtagacctt gacgaagcat gggcgttctt aaatgtggca caaggcgaaa
    27421 cgctctctaa caagctggtt cgtgctggac gtgccatgaa cgcaggcgtt tattttgtta
    27481 cacagtcctc tggcgacgtg tcaaaggaaa gtctgaaaaa caatatcggt ttgaaatttg
    27541 cctttagaag caccgatatt aacgaaataa aacagaccct tgaatttttc agcattgaca
    27601 aagaggacga gaacaaccag aagcgattgc gtgatttgga aaacgggcaa tgcttgcttc
    27661 aagatttata cggacgtgtg ggcgtggtgc agatacaccc agtctttgaa gaattactgc
    27721 acgcctttga taccagaccg ccgatacagc gaaatgaggt ggagtgatga aagaaaggat
    27781 aaaaagtgtg ttcacaagaa aaaagatatt ccgctttctc aaaatggctc tgtttgtcgt
    27841 ggcactttcc cttatcctgc tttcactttt gggaacggtg gcacacgcaa cgggacttgt
    27901 ggacgacact atcaacaccg agaaccttta ttccaaatat ccgctgtcga attatcagct
    27961 tgatttctat gcagataata actgggcgtg gttgccgtgg aactggctgg acgggattgg
    28021 aaaaagtgtg caatatgggc tttactgtat taccaacttt gtctggacga taagactata
    28081 tctaagcaat gcaacgggct atgtagtgca ggaagcctac aagctggact ttatcaacga
    28141 tatggcagac agtatcggaa gaagcataca gacccttgca ggcgtaactc aaaatggttt
    28201 ttctacttcg ggcttttatg tgggatttct tctgctgatt atcttagtcg taggtatcta
    28261 tgttgcctat acgggactga taaaacggga aaccagtaag gcactacacg ccgttatcaa
    28321 ctttgttgtg gtgttcgtat tatcggcttc ttttatcgcc tatgctcctg actacatcaa
    28381 gaaagtgaat gacttttcat cagacatcag taccgcttca ctggacttgg gaacaaaaat
    28441 catgcttccc gacagcgaca gcaagggcaa ggacagcgtg gacttgataa gggatagcct
    28501 attttccata caagtacaac agccgtggct actgcttcaa tttggtaatt cgggtataga
    28561 ggaaatcggg gcagaccgtg tcgaagctct cgtatcggca agcccagagg acgaggacgg
    28621 caagaccaga gaggaagtcg tgaaaacaga aatcgaggac aacgacaaca acaatctgac
    28681 gatactgcag gtggtaaacc gtttaggtat ggtgttcttc ctcttgttct tcaacttggg
    28741 tatcacgata tttgtattct tgcttacggg tatgatgttg ttcagccaga tactttttat
    28801 cattttcgct atgtttctgc ctatcagctt tctattaagt atgataccga gctatgagag
    28861 catggcaaaa caagccatag tccgtgtgtt taatacgatt atgacaaggg caggtattac
    28921 actgattgtc acggtagcct ttagtatttc cagtatgttc tacaacatat cgacggacta
    28981 cccgttcttt atggtggcgt tcttgcagat agtgtatttt gcaggtatct acatgaagct
    29041 gggcgattta atgagcatgt tctctttaaa cgctggcgac agtcaaagca tgggacggcg
    29101 aatattccgc agaccgtatc tgttcatgcg acatagggct agacgtatgg aacataggct
    29161 tgcaagagct gttagtgctg gtggtgtcgc tggtgcggtg gctggtggtg tcgttgccag
    29221 ttcaaaatct acacgccaga atggaacaaa gaacaatcgt ggaaatacaa gctccagcat
    29281 ggggcagaaa gcaggttcag ccgtgggagc tgtccttgat acaaagaata aggtcaagga
    29341 caaggcaact gctgtcaaag aaaatatcaa agatatgccg acacagactg cctatgcggt
    29401 tcactctgct aaggaaaagg cgaagtccag tgtgtctgat ttcaagcgtg gcgtggtgca
    29461 ggaaaaggaa aaccgccagt ctggacgctt aaagaagcaa cagctacata gacagaatat
    29521 cgctgacaag cgtatggaac ttcaaaaggc acaagaatca aggcaggcag gtaagaaaac
    29581 ggacggatca gcgacaagcg gagctacccg tccccatgaa agaccagcta ccacttctaa
    29641 ggttaatgta gaaaaacagc aggaagtcaa acgccctgcc atagcttcaa ccgtgaagaa
    29701 tgatgaacct atcaagaaaa atgttgtcaa ggaacgtcca ctatcttctg gcgatacatc
    29761 agcaaagacc gtcaatcaag aaaccacaag acagaattac aagactgacc gtgttacgaa
    29821 aacaaccgtt cagaagcata cacagcagaa taccgagaag aacaaacgaa ccgtattcaa
    29881 gaaaggacaa aagaagaaat gaaactaaga catatcgcta ttgttggcag tctgttttct
    29941 atcctctttt ctctcgtatt attctttggg gttctgatta gtgccgagga cgacgacggg
    30001 ggcaatggtt attcctctaa ttatgtaggt atgaacctat ctgccgaagt cttgaaacat
    30061 cagcctatgg tagaaaagta tgccaaagaa aacggtattt ccgagtatgt caatgtgttg
    30121 ctggtaatca tacaagtgga aagtggcggt acagccgagg acgttatgca aagctctgaa
    30181 agtctgggac taccacccaa ctcactagat acggaaagct caatcaagca ggggtgtaaa
    30241 tattttgcgt ccctgctttc ttcctgcaaa aatcaaggta tcagtgatat aaatgttgtg
    30301 atacagtcct ataactatgg cggtggctat gttggctatg tggcaggaaa cggaaaaaag
    30361 catatgttca accttgcgga aaacttcgca agggataagt cgggcggtca aaaagtaacc
    30421 tatacgaacc ctatcgccgt ggcaaaaaat ggtggctggc gttatggcta tggaaatatg
    30481 ttctatgtgg agcttgtgaa ccagtacctt gttgtcaatc aagtatcggg cgagctggca
    30541 caaaaggtta tgaatgaagc cttaaaatat caaggttgga agtatgtata tggtggcagt
    30601 aacccgaaca cttcttttga ttgttcggga ctggtacagt ggtgctacaa caaagctggc
    30661 gtatctttac cgagaaccgc acaaaatcag tatgacgcaa cccagcatat cccactatcg
    30721 caggcaaagg caggcgattt ggtctttttc cacagtacct acaatgcagg aagctatgta
    30781 acccacgtcg gtatctatgt aggaaacact cagatgtatc atgcaggcga ccctatcgga
    30841 tatgccgatt taaacaatgc ttattggcaa cagtacttga tttgtgctgg aagaataaaa
    30901 cagtagaaag gattggaaac catgtttaaa aagaaagaaa aaccagtaaa agaaacgaaa
    30961 gtccgcacta tgaaagtagg cacacataag aaatctgtga ttgctctgtg ggtggtgctt
    31021 atcgccagcg tatcatttgg cgtgtataag aactttaccg ccattgatat gcacacggta
    31081 catgaaacgg aaaccattca gttaagacta aatgacacca acggagttga aaattttgtg
    31141 aagaacttct gtaagtccta ctacacatgg gacaacagca aggaagctat cgaagcaagg
    31201 acgcaggcaa tcagcaacta tctgacaaag gagttgcagg acttgaacct tgataccatt
    31261 aggacggata taccgacaag ctccactgtt acaaatgtac tgatttggga tataaaacag
    31321 tctggaacga acgattttat cgctacctac gaagtagatc agcagataaa agagggagaa
    31381 cagacaagca atgtcaaagc aacctatacc gtcaaagtcc atgtggataa agacggcgat
    31441 atggttatcg tacagaaccc aaccattgca ccagcgattg aaaaatcaga ctatgagcca
    31501 aaggcacaag aagccgacgc aagcgtggac gcagataccg tcaatgacgc taccgcattt
    31561 ttggaaactt tctttaagct gtatccgaca gcaacagaaa aagaacttgc ctactatgtg
    31621 gctggcaatg tgcttgaacc tatcggcagg gactacctct attccgaatt agtcaatcct
    31681 atgtttacgg atgacaggga taatgtgaaa gtcagggttg cggtgaaatt cattgataat
    31741 cagacaaagg caatgcaggt atcacagttt gagcttgtgt tacataagga tagcaactgg
    31801 aaaattatta tgtaagatat tttgttggca ggctctattt tcggatatag tctgcctttt
    31861 ctatagcaaa tgtgctgcac taacaagcga tttcattgta ttttaagcaa ttaaatcttt
    31921 cttgacaatt ttatcttaaa tcatatattt atggatattg actttctgct tgcaataagt
    31981 tataatatga ctataaaact aaattagtca tataggaggc gtgtggtatg tataaagaag
    32041 ttggtatttc ttcaaataat gttccgataa cactttctat ttggtataca gaaagaaata
    32101 gtcctaccat tgtttttttg ccgggtacta tgtcacaccc tctcatgtat gagaattttc
    32161 tatgtggttt gagtgaaaga ggatttaata taataggcat tcactatctt tcacacggaa
    32221 aaagccctaa aatcaaaaaa aattatacaa tggaagattt gctgcataat gtatatgacg
    32281 caacgactta cggaatagaa aattttggtg aaaacatagc tgtcatgggt tccagtcaag
    32341 gtgggatttt agctgcaatg gcggcgggta gggacacaag gttaaaagcc gtttttccac
    32401 ataatatcat gctgactaca ttaaaagaaa ctatgagctt aacaaaatac cccgcatggt
    32461 cacaccgttt tatggggctg atacagttgg gctttcgtgt aggtggaaaa ttgctgccta
    32521 actataaggt tccaggagac gcttatttag actatgacag agtattttat agtgaacagg
    32581 caaaacaaga ttgcttaaat gaccctatgc ttttacccta ttatccttta agatttgtcg
    32641 ccagcttatt taatgctgac ttatcgtgct tggagaatgg ggctatacaa tgtcctgtta
    32701 ttttaattac tgccaaaggc gatccgcttt ttagatttga ttataccaac aaagtatttg
    32761 atttaattca tgtaccgaaa aaggaattac ttactttaga gcttaatcgt cacatgattt
    32821 ttaatgagga cacggacact acaataaacg cattggaagc taccttgcat aaatatcttg
    32881 gatagtatat tgattatata atcagtaaat gttatacttt tcatgacgat aaaaacattt
    32941 tagtcataag ggggttatga gtttgccacg atttacggaa caagaaaaag aaatcataaa
    33001 cagtaaactt ttgatagagg gagaaaagct gttttcatta tacggcttaa aaaaagtaac
    33061 agttgatgat ttagcagctg ctgtaaacat atcaaaaggt tcattctatg ctttttatcc
    33121 cagtaaagaa catctatatg tggaaatcaa ttttcgctta caaaaagagt tatttacaag
    33181 tatagaaaca actgttaaaa agaaaaagta taagaaccat agagatttag caaaggacgt
    33241 tattatgcta agtttaacgg gggtgattac ttcgcctatt ctttcacaga ttgatttatc
    33301 tttaatggac tatctgcaaa gaaaaatatc gtcagatatt tttgaaaatc atatgtacag
    33361 tgatattcgt atattggaga tactggaagg tttaggggtt gtatttttgg ttccgcatac
    33421 ggttatcata aaatccctgt attctgtttt atcctgtttg gaacaattca aagaggacga
    33481 agaattgaat atggtacaaa atcttttagt gaatggtatt attcagcaag tagtagcaga
    33541 gtaataattt tgtttggtta tttgacaggc aagtggccaa acaaattttt tacagtttat
    33601 atgactatat atctataaac gtcataaagt taaaggagga aagaataatg attgatgtaa
    33661 aaaaactgta ttttagttat actgacaaac cgtttgttga gaatgtaagt ttccatgttg
    33721 ggagaggaga aattttcggt tttttaggac cctctggtgc tggaaaatca actattcaaa
    33781 aaattttaac tgggcttaat acaagttata aaggcagcgt aaaagttgca ggtacagaaa
    33841 taagggagca tacaaaccgg ttttatgaaa atattggagt agactttgaa ttttcaacct
    33901 gctacgagaa attcacagca cgacaaaatc tcgcctattt tgcttctcta tatgacaagc
    33961 aacctcggtc tattgatgaa ttattgcata tggtaggctt agaaaatgat ggggacaaaa
    34021 aagttgctga cttttccaaa ggtatgcgtt ctcgcttgaa tttcattaaa gcgttagtac
    34081 atgaccctga tatattattc cttgatgaac ctacaagcgg tcttgaccct acaaataatc
    34141 gtttaatgaa agatattatt cttgcggaaa agaaacgcgg aaaaacgatt atcattacta
    34201 ctcacaatat gtatgacgca acagagctat gtgaccaagt agcctttatt gttgccggaa
    34261 aagtcagtgc attggatagt ccacataatt tgattatgtc aaggggagct gccaaaatac
    34321 actataccta ctatgataaa ggcgagaaaa ccggagaatg tctgcttgat agaaccacag
    34381 aagataagtt gttaaaaagt ttaatttccg aaaaccgtct gctttctatt catagtagtg
    34441 agcctacatt aaatgatatt tttgttgata ttacagggag gacacttcaa tgagattggg
    34501 acgtttaatt tgtggtgata tacatttcca atggaaatat ggtttctatt ttatctactt
    34561 tatattaaca gttttatatg tgtgtgggat tgccgctttg acgggacatt ggaaaacgga
    34621 tattgcttct atcatgatat actctgaccc ggcagctatg ggattgtttt ttatgggggc
    34681 gattgtcctt ttagaaaaaa gccaaaaggt attaaatgct atggtagtat cgcctgtaaa
    34741 agtatcggag tatatacttt caaaaaccat cgcattgatt gctatttcta caatcattgc
    34801 ggtgatattg ggatttgttt ccggcagcaa tcatttactt ggtatcgcag ttggtacagc
    34861 tttgacatca gcaatcttta ctatgcttgg cattatcgcg gctacaaaaa tttcaaactt
    34921 gaaccaattt ctcattgtga ttatgccgat agaaattgtt tgctttgttc cacctatcgt
    34981 tggtctgttt gtaaagttac cagatatatt ccgctttttt ccgttcacag cttgcatgaa
    35041 cctcataact ggaaaaagca ttttactatc gtttgatatg gtacttgtaa ttgctacgtt
    35101 gattatcctc tacatagttg cgcgacatac agttaagcat atgtggagaa gtttgggagg
    35161 tgtaaagcta tgaaaaggat attatccagt accatacagg tatttaagca aatcaagagc
    35221 gaccctatga tgtttgcagc ttgttttacg ccttttgtca tgggagcttt gattaaattc
    35281 ggtattccct ttcttgatgg aataacagat ttttctttgc aaacatacta tccgatcttt
    35341 gatttattac tttctatcat ggctcctgta ttgctctgct ttgcatttgc aatgattaca
    35401 ttagaggaaa tcgacgataa agtgtcacgg tacttttcga ttacccctct tggtaagtcg
    35461 gggtatctct ttacaaggtt gggagtaccc tcaattattt cggcggtcat cgcttttatt
    35521 gtactgttgc ttttttcatt agaaaagttg tccattggaa tgacaatatg tttggcgctt
    35581 ctcggctcag tacaggcaat tattgtttcg cttatgatta taacattatc aagcaataaa
    35641 ttagagggta tggcagttac aaaactagct gcacttacat tactcggaat accagctccc
    35701 ttttttattg atagttacta ccagttcgcg gttggcttcc ttccatcatt ttgggtagcg
    35761 aaagccatgc agaatgaagc ggttctttat ttctccatag gattgatggt agctttggtc
    35821 tggtactact tccttataaa acgtctgttt aggaagctgg caggataaga aggcatatta
    35881 tggaaaaagg tagacttctg caaaacgaaa atataaataa ggctattcag gtttccaaaa
    35941 tcacactaat catcaacctg ggtttatcgc tacttaaatt tgctgccgga tatataggaa
    36001 agtcaagtgc catgctgtcg gacgctgtac actcggcttc tgatgtatta agcacaatcg
    36061 ttgtgatggt tggaattaaa atatcagaaa aacagcctga taaagaacat ccttatgggc
    36121 atgaacgtat ggaatgtgtg gcttccatta ttctctctgt tgcattggct ataacgggtg
    36181 ctggaatagg atattcaggt atcaagaaaa tcttttcagg acagtacaat acgctttctg
    36241 tctctagtgg aatcgcactt acggcggcag tcttatccat tgttataaaa gagtggatgt
    36301 actggtatac aagaagtgct gcaaagcata caaattctga cgcacttatg gctgacgcat
    36361 ggcatcaccg ctctgatgca ctctcgtcgg ttggttcctt aattggcatt ttaggagcac
    36421 gtttaggcta tgctattctc gaccctattg caagcattgt gatttgcggt tgtatcttaa
    36481 aagctgcttt ggacattttc aaagaaagca ttaacaaaat ggtagaccat agttgtgaca
    36541 atgctactga aacgaagatt agggaagttg tgcttcagca gcaaggggtt gatggtattg
    36601 atgaattaaa aacccgcatg tttggagcga aaatgtatgt agatattgaa atattagctg
    36661 atggtaattt agctctttat gacgctcata gaattgctga gggagtccat caaacgattg
    36721 aaaacaactt tccacaatgt aaacattgta tggtacatgt taatacgaat gaacttccct
    36781 attctccata ggacagggaa gatgatttaa gactattgaa agggaaagga ggggaagtag
    36841 gttatggaac aatactggtg gattatcatt gttatggttt tgattgtcgt tggcttactt
    36901 aaaattgtaa ttatgaagaa gtataaccaa aggagaaatt ctaacaatcg ttcaaaaaat
    36961 agcgaggatg acaagatgta ttaagagcaa ttatccgatg gtatgagata tatacctatg
    37021 aaatttgaac gataaatccc agtttgtaaa agtcaataaa tccaaagata aatttgtatt
    37081 tgaaaggagt tttgaagaat gaaaaaatat gagtatgtag aaattaatta taatgcgaaa
    37141 ggagttgtat tcctttgtac tgataaacac aaagaaatta ttgacagtta tgcaaaaacg
    37201 gggtatcact acgcagggtt tgtacccaca gaaattgacg caaaaggctg tatgcgaaag
    37261 atagatttaa tttttgaaaa agaagattaa cttattttag ggagttggtt ctaatgatag
    37321 gacttaaaag acgagatatg aattatatta tgaaagttgg tgctaaagct caaggtgttt
    37381 ctgtatctga atttagggct atgctacaag ataccataga caaaactatg gaaagtacag
    37441 acccagacgt gcaggcaaat tttaaacgat actttggaaa taagcggcca acaccagagg
    37501 aatatattta tacgattacc aaaaagacaa aggttaaggt ttaaatgttg taaagggggc
    37561 ttgccgtatg aaacgcttta aatacaattc agtatattca agcccaattt aacagcatac
    37621 tatttacaat taaatacgct ataccagtag ctcgtacaaa atttatattt tgtgcgggct
    37681 tttttaatac ccgaaaagtt ttttaattat tttcattttt ttggaagcaa atcacacctt
    37741 gctgtacaga tatggtgcgg aaagagggat aaacactttt caattcatag ctggaaagga
    37801 ggtgagatta tgaaaccgtc tgacttccaa aaaacaattc agtgtcagtt tgaatgtaag
    37861 ctgaaaaaag ttgtcaaagg tattatctgg aactaccgca aagaattaag acgaagaaaa
    37921 aacaaggaaa tatcttttag cgaattacca gacattacgg tagaaagtct ggcaacatgg
    37981 gacgactacg aaacaaacta cacaattttt aatgtgtgtg gcatagatat taaagtcctt
    38041 gatgatgaac ttgcagaagc tctcaaacag ctacccgaaa aaaacgtaac aatgtattga
    38101 tgtattactt cttggaaatg agtgatacag agattgcaga tattatgaat tataccagag
    38161 aggctgtgtt caaacacaga caccgtgcac tagaaactat gaaagagata ctaaatgaaa
    38221 agacaagaca gtaaaggacg caatttgcgt cttggagaga gccagagaaa agacggaaga
    38281 tacgtttata aatataccga tatattcggg aagccacagt ttgtatattc ttggaagctt
    38341 gtacccacag acaagacacc tgctggaaaa cgtgatgata tatcgttgag ggaaaaagaa
    38401 aagcagataa aaaaagacct tgacgacggt atcgacacgg tgggcggtaa aatgacagtc
    38461 tgccagcttt acgaaaaaaa gaacaatcaa agaaagaaca tcaagagaaa cacggaatta
    38521 ggacgacagt atcttgtgaa cgcactggaa aatgacccat tgggtatgag ggcgattgat
    38581 acggtcaagc agtccgacgc taaagaatgg gctatcagaa tgaatggcaa aggttattct
    38641 tacaagacga tagataacta taagcgttcc ctaaaggctt cattctatat ggcgatacaa
    38701 gacgactgta tcagaaaaaa tccttttaat tccaagttga gtgatgttct ggaagatgat
    38761 acggaagcaa aggttatcct gacaccagag caggaagaaa aactccttgc ctttatggaa
    38821 acagataatg tttacagcaa gtatcgtgat gaagttattc ttctgctgga aacgggactt
    38881 cgtatctctg aattatgcgg actgacaaca cacattgata tgcttaaccg tgtgattaac
    38941 atagaccacc agctattgcg tgatacagag gtgggctact atatttccaa agctaagaca
    39001 aaaaacggta agcgagagct tccattaaca gaaagagcat atcaagcact caagcgaatt
    39061 ttaaagaacc gaggaaaagc acaaccgctt gttgttggtg gttatagcaa tttcttattc
    39121 ttgaaccgtg agggattgcc aaaggtagca ggaaattatg aaagtatggt gcgtgggttg
    39181 attaagaagt acaacaagac ccataaggac aagttaccaa acatcacgcc ccattcattc
    39241 agacatactt actgtacgaa catggcaaat aagggaatga acccaaacac cctgcaatac
    39301 atcatgggac acgcaaacat aaccatgaca ttagggtatt atgctcacgg tactttccag
    39361 tctgcaaagg cagagttaga aaggctggct tcttaatatc gaaaatgtat cttactactt
    39421 atttactact tttggttgca ttttcatgct ggtaaatgcc agcttatgca aggtatcttc
    39481 caaaaagaaa atgcccatac agcccgaaaa tgcgggatat atcggcatct accaacttat
    39541 gaaaagataa tcagaaattt agtaagtttt tataataaaa aaataagagg ctgtccaaaa
    39601 atagagataa gccttttttc ataatcggaa ttataaaaat acacttgatt tcatatgaaa
    39661 aagagtgtct cataaactaa aaagtttact ttgagacact acctttttta ttcttattta
    39721 tactttgtag acatattata atatgcgttt cactggtatc tgaacttctg tcaaccagtc
    39781 ttcttcactc tctttattcc atataccatc tatataagat tctctaggat ttccaaccac
    39841 tttatattta ttgtcctcta tccatttaaa tgcatctgca taagtttttc ctaatgttga
    39901 atatggacca ttgtgtaata tacaaactgc attttcaact gcatctaatt ctttaaattt
    39961 taatatatcc gtatcttcct ttaaatcatt tatagcttga cacatttcaa catccatatc
    40021 agtctctcta tattcaccat catgatatat attaaaacaa taatcaggaa ctgcacatgt
    40081 acaccctgtt ttcatcattt cttcagccat tacatttgga aatagattaa aaagttcatc
    40141 atatcccttt aacacttttc tcatagaagc aactttaact tttggtaaag acttaataat
    40201 tacatcattc atattgactt ctcctttcaa attcataagg taattacgta gtttaattat
    40261 tttatattct tcacttttaa ttacatccaa catttctttt tctttattca ttaaaatatt
    40321 ttcaacttct tcaggatttt ctacagcttt ttttatctca gctaatgata aacccatttg
    40381 ttttaaagtt attatctgat gtaatttagg taattgctct gttgtataat acctatattt
    40441 tgtaaattca tctacatatt ctggttttaa caaccctata tcttcataat accttaatgt
    40501 ctttgtagtt attttattta ttttagaaaa cattcctata gaaaacatct catcacctgc
    40561 gcattagatt tgattaattt aagtttaaac tttcccctta ggttaaagtc aatacaaaat
    40621 aaattataat tgtattaatt cttcaattct atctccactc aataccaaaa tatcccctgt
    40681 ttttataatg gttgaacctt taggtattaa aacctgttcc tttcttttta tcataactat
    40741 taatatatcg tctggtatat tagaatctat tatactctta ttagcccatt tattaccctc
    40801 atcaacattt acttcgataa gtttagagct aatttcccca tcataatcat taaatgtttt
    40861 taatacaggt gtattatcat caactaaatc caactttctg gctacagttg gtatcaatga
    40921 accttgtatt cctactgaaa gcaacgctat aaaaaatatt atatggaata tgtcattctc
    40981 tatggctact ccttgagtaa cagcataaat tgcaaaaact actgaagccg cacctctaag
    41041 acctacccac gatatgaata atttttcttt atttgagaat ttaaattttg ctagaactat
    41101 aaatgttgct aatggtcttg caataactat catgaatata gatatggcca ttgctatacc
    41161 aaatacattt ggtaacttag aagggaaaga taacaatcct aacataaaga ataacattat
    41221 ttgcataatc catgaaattc catcaaagaa atgaaccaaa ctttttttaa gaggtatcct
    41281 actatttcct ataataatac ctgaaatgta aacacttaaa tatccatttc ctccaagata
    41341 ctcgctaaat gcataagaaa gtattgcaat agctgttata aatatagtat aaaatccttc
    41401 tatctcgaag tttgaatgcc ttaaaatata aacagataat tttgctagta taaatcctat
    41461 agctaaccca aaagctattt gttttattac cattggtgca ataagttcta ccccagaatt
    41521 attcatcaat gtcaaaagaa ttagtgtcaa catatacgct attgggtcat tacttccact
    41581 ttcaacttct aataatgatg caagtgaacc ccttaagttt agtttttgtg aacgcaacac
    41641 agcaaacaca gatgcagcat ctgttgaagc tgcaattgcc ccaattaata aaccttcaag
    41701 taaactagtt cctattacca tgtggcaaaa catacctgta accccagacg taattattac
    41761 acccagtgaa gacattaata tagaaggtat agcaacaggt ttagccatat tccagtttgt
    41821 tccaaaccct ccaaaaaaca ttataaatat tagtccaata gaacataatt gttttgttaa
    41881 ttcatagttc tgaacctccc atgactaaag tcacagagtt cctgcttcat agaattttgc
    41941 atactaaaat atgtcttact aattctccac aggctatccc catagttcct acggttctta
    42001 cataagttat ttattttgaa ttgttattag tcttaatcct tcttttaata tatttatact
    42061 tgcatttata tctctatcgt gagtttcatt acaactagga caaatccatt ctcttatatt
    42121 taagttcttc acttcctcgt ttttataccc acatttattg catatttgtg aacttgcaaa
    42181 gaatttacct acctttacaa tttgtctacc atgccaatta
//
LOCUS       DAELSY010000016        41170 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.016, whole genome shotgun sequence.
ACCESSION   DAELSY010000016 DAELSY010000000
VERSION     DAELSY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41170
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.016"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(273..1340)
                     /gene="rsgA"
                     /locus_tag="KPG09_000786"
     CDS             complement(273..1340)
                     /gene="rsgA"
                     /locus_tag="KPG09_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG0965412.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKSGCTVALLGSSGVGKSSLINC
                     LLGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKTIRR"
     gene            complement(1337..1738)
                     /locus_tag="KPG09_000787"
     CDS             complement(1337..1738)
                     /locus_tag="KPG09_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBG0965413.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     regulatory      2534..2630
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2684..2860
                     /locus_tag="KPG09_000788"
     CDS             2684..2860
                     /locus_tag="KPG09_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965414.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            3270..3908
                     /locus_tag="KPG09_000789"
     CDS             3270..3908
                     /locus_tag="KPG09_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965415.1"
                     /translation="MSNQIKDFDITNDMFQESIPITTKTILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLVKAANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(4386..5744)
                     /gene="rlmD"
                     /locus_tag="KPG09_000790"
     CDS             complement(4386..5744)
                     /gene="rlmD"
                     /locus_tag="KPG09_000790"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG0965416.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAEGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVAKII
                     KQ"
     gene            complement(5919..7679)
                     /gene="pyk"
                     /locus_tag="KPG09_000791"
     CDS             complement(5919..7679)
                     /gene="pyk"
                     /locus_tag="KPG09_000791"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBG0965417.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEILTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(7731..8690)
                     /gene="pfkA"
                     /locus_tag="KPG09_000792"
     CDS             complement(7731..8690)
                     /gene="pfkA"
                     /locus_tag="KPG09_000792"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG0965418.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(8899..12468)
                     /locus_tag="KPG09_000793"
     CDS             complement(8899..12468)
                     /locus_tag="KPG09_000793"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBG0965419.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(12625..13572)
                     /gene="whiA"
                     /locus_tag="KPG09_000794"
     CDS             complement(12625..13572)
                     /gene="whiA"
                     /locus_tag="KPG09_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBG0965420.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(13589..14014)
                     /locus_tag="KPG09_000795"
     CDS             complement(13589..14014)
                     /locus_tag="KPG09_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG0965421.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(14083..15189)
                     /locus_tag="KPG09_000796"
     CDS             complement(14083..15189)
                     /locus_tag="KPG09_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBG0965422.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(15201..16058)
                     /gene="rapZ"
                     /locus_tag="KPG09_000797"
     CDS             complement(15201..16058)
                     /gene="rapZ"
                     /locus_tag="KPG09_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBG0965423.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIDEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(16089..16802)
                     /locus_tag="KPG09_000798"
     CDS             complement(16089..16802)
                     /locus_tag="KPG09_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG0965424.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYNDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFKLA
                     MKTISDSNLDISLVENIII"
     gene            complement(16806..17720)
                     /gene="murB"
                     /locus_tag="KPG09_000799"
     CDS             complement(16806..17720)
                     /gene="murB"
                     /locus_tag="KPG09_000799"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBG0965425.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(17993..18763)
                     /locus_tag="KPG09_000800"
     CDS             complement(17993..18763)
                     /locus_tag="KPG09_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG0965426.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
     gene            19081..20562
                     /gene="cls"
                     /locus_tag="KPG09_000801"
     CDS             19081..20562
                     /gene="cls"
                     /locus_tag="KPG09_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG0965427.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
     gene            20880..21377
                     /locus_tag="KPG09_000802"
     CDS             20880..21377
                     /locus_tag="KPG09_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBG0965428.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLTTKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            21394..23280
                     /locus_tag="KPG09_000803"
     CDS             21394..23280
                     /locus_tag="KPG09_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBG0965429.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            23303..25084
                     /locus_tag="KPG09_000804"
     CDS             23303..25084
                     /locus_tag="KPG09_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG0965430.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRICMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(25224..27020)
                     /locus_tag="KPG09_000805"
     CDS             complement(25224..27020)
                     /locus_tag="KPG09_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBG0965431.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQILSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLSPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVISHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(27234..28163)
                     /locus_tag="KPG09_000806"
     CDS             complement(27234..28163)
                     /locus_tag="KPG09_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBG0965432.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            complement(28163..29980)
                     /gene="uvrC"
                     /locus_tag="KPG09_000807"
     CDS             complement(28163..29980)
                     /gene="uvrC"
                     /locus_tag="KPG09_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBG0965433.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVEAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            complement(30239..33064)
                     /gene="uvrA"
                     /locus_tag="KPG09_000808"
     CDS             complement(30239..33064)
                     /gene="uvrA"
                     /locus_tag="KPG09_000808"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG0965434.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            complement(33083..35053)
                     /gene="uvrB"
                     /locus_tag="KPG09_000809"
     CDS             complement(33083..35053)
                     /gene="uvrB"
                     /locus_tag="KPG09_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBG0965435.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            complement(35240..36502)
                     /locus_tag="KPG09_000810"
     CDS             complement(35240..36502)
                     /locus_tag="KPG09_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBG0965436.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKVKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFDLKLDGVEKEAFDFIM"
     gene            complement(36565..37869)
                     /locus_tag="KPG09_000811"
     CDS             complement(36565..37869)
                     /locus_tag="KPG09_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0965437.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INRDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            complement(37900..38760)
                     /locus_tag="KPG09_000812"
     CDS             complement(37900..38760)
                     /locus_tag="KPG09_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG0965438.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSMEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLILQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYIPLIIPFMTS
                     SFGIFLLRQNFMQISDETIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            complement(38804..39673)
                     /locus_tag="KPG09_000813"
     CDS             complement(38804..39673)
                     /locus_tag="KPG09_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG0965439.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            complement(39657..40727)
                     /locus_tag="KPG09_000814"
     CDS             complement(39657..40727)
                     /locus_tag="KPG09_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0965440.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGKLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
BASE COUNT        12953 a         7226 c         5091 g        15900 t
ORIGIN      
        1 catatactct attatgtatt agtataaata tgccttttct tatcttacat aagacctctt
       61 tataaaggag actatcgtaa caaatatgta tacattatca catatatata tcatcatata
      121 aaaaatcaaa ctttattaaa aaaattaata aattgcttta aataatacca caaaccaaat
      181 ttgccatcaa aatcacatta aagtaacaaa ttaatggcaa atttaataca ttaatagtat
      241 atagactcat agaatgaaca aaaaaactac acttatcttc taatcgtctt atttattttt
      301 gagatatttt taaatttctt ttcttttttt gctaagtatt cgcttttatt gtctgcaaat
      361 actgcttcag ttttaagcct acgataggat tcccatcttt taggcgaaag ttcttgatga
      421 gcaatagctt cccagatagc acagcctggt tctgaggtgt gagtacaatt acgaaaatga
      481 cattttccga aataagcttc tatatctgca aaacttttat ctaaaccttc tgaaacatcc
      541 cacattccca actctctcat acctggtgtg tcaataacaa taccaccacc tgaaattaaa
      601 aatagctctc gtctagtcgt ggcatgtctt cctttatcat catctatgcg aatttccttt
      661 gtatttaaaa attctcctcc tagcagacaa ttaattaatg aagattttcc aactcctgaa
      721 gagccaagta atgcaacagt acagcctgat ttgatatact tagaaatctg tttataacca
      781 tctggctcta tagatgaggt aactactaca tctacaccca aggctataga attaactact
      841 tgtaatttac gatgcacatt gtcacataag tctgatttag taagtactac tactgggata
      901 gctccacttt cccatgttaa agacagatac ctctccaatc tatgaatgtt aaaatcctta
      961 ttcagtgaca tgcacaaaaa aacaatgtca atattagctg caaccacctg ttcctcattt
     1021 acctttccag cagccttacg cacaaacaag ctttttctat gaagcacatg atgaataata
     1081 gcattaccat ttttattatt gcaacgatcc aacataacga aatcaccaac tgtgggaaaa
     1141 tcagattgta tagtagcctc aaaacgcagt tttcctgaaa cctctgcaaa cagtacacca
     1201 tttacacata cagcttgata aaagttcttt tcctgcgaga taattcgagc aaggtataaa
     1261 tcgtgatatt ccatactttg agtaataaaa ttcctattta gaccatagtc ttgtaaattg
     1321 atttgtttca taccattcat tctgtttcct ccttttccaa cttttcaatg attctttcta
     1381 gtgggaaagg gggctgtgcc agtggtttta atgcaattga cattaatttc actgaattat
     1441 catcagctgc aatacgatta gaactaagat gtccacagcg tgtacaacga tgaataattg
     1501 cccactcacc attttttctc acccacactc caataggttc catattagca tcacaatctg
     1561 cagcacggtc accaggaatg atatctacat gaagactatg taaacaatgt ggacaatgat
     1621 ttctatgttg tgtaccagct ccttcaggaa ctacaaggca accacaattt ttgcaaataa
     1681 agttatccat acaggaattt tcctgataat aagctcgttt tctattttta taattcattt
     1741 ttatttcctc cgtatcttgt attttctaca aaaaaagtcc acaggtattt atatctgcct
     1801 ttttatgtgg tatacatatt tacctacaca aaaacagaaa acccctgtgg actagaaaat
     1861 ttagtatctt ctatttcaac ataattatat tcaaaataat actgtgtaaa attaataaaa
     1921 gtgtagcaga ttaaagccca ctacaccaca atttaacaaa gtaatatgta atattattat
     1981 ttgttgtcta atgttagtta tctataaagg cagagtaaga cacaacaatc aaatgtatca
     2041 aaaatacact taaaaattct gattaaacag aacccatatg cgtctccaac tatttatttg
     2101 ttggttacca ttatatataa ctctgacatc aggcaagaca ctccttcact aataattatt
     2161 attaaaattt tatcataaaa tataagtatg ttcaagtata atttaatata ttcatatatt
     2221 tatgtttgtc tcattttctt attctataaa atctagtggt attttaaaat ttaatgttca
     2281 cctagtatta ataataaaaa ttaattgcta ttatttgttt tctcatttta tgcctattgc
     2341 tcaatttatc tcacttaaat taataacaac tgtcttttca tacaaaacct acaatcaagt
     2401 ttttccatat attctacatg aatagtctat tttttttata taaaataacg ttcatatttg
     2461 taaatttatt taattttcaa ataagtatat tgaataataa gcaattctat tgtattattg
     2521 taattgtaga ataaaactaa atcggcaaaa ctagagaaat ttagtgacgc aaagctatag
     2581 ggactaacgc ttataaaata aactatgagt tatgtcagcc agttgccaaa aagagatatt
     2641 ctttttgttt ttatgaaagt tttttagggg gataataata tatatgttta gagataaaat
     2701 ggatagatgt acacatatgt tgactgctta tattggtagt tcatatgatt attgtgattt
     2761 tatagataca caactagatg attttatact agagtacgga gaaaatgtgg tagaatcttg
     2821 tttgcatcaa gtgatggtat tagtaagtaa gtataattaa atagatttgg ttttctagag
     2881 aacccttttt agttttatct actttaaata gccttatgtt agaaagctaa ttaagataga
     2941 tttctagtat tcataatttt attttctatt taaaatacat ataataaaga tgtaaatttg
     3001 tatcaaataa taaatatgag aacttaaatt ctcttatcta tgtgaaaata aacatctctt
     3061 aaaatgaaat tctgtaaaca taaatgattt tttagtttga aggattagat taacaaaatt
     3121 ataaatttgc aaataagcta tattaaaaaa tataataatt ttttatataa cttattcttt
     3181 tataactttt aattttaaaa gaggagaatg tatataagag tattcaatgt actagaaaaa
     3241 atcaaaagaa atattagaag aaatggatat tgagtaatca aataaaagat tttgatatta
     3301 caaatgatat gtttcaagaa agcattccta tcacaacaaa aactattctt gaagacatgc
     3361 cttcaaatga tgaattaaat catgtttttt caaaaggttg tgaacgaaaa atgaaaaaga
     3421 gaaaaattgt attaatcaca ctactattaa taggtgtttt gttattagga agtatcctat
     3481 ataatctttt tttagtaaaa gcagctaata tttcaatgct aaaggaatct tggaattttg
     3541 atataccaat acctaacaaa gaaattgaag tatttgatac tcaagatagt attaatggag
     3601 atggacaaag ttattttatt caaggatttt ctgaaaagaa tttcaaaaaa gtttttaatt
     3661 taaaaggtgg tatagttgta agcaaagata atattaatga aatagaaaaa tatattgata
     3721 aatttaaaag agattctgtt aacattaata aaagtaataa aaataaaatt gaagaagatt
     3781 ttaaaaaata taagttagaa gttaaaaaag atgataagta tatatataag agaaattatg
     3841 aaaactatgt agttttaatt ataaaaaaag atgaacaaaa attatacagt ttaatatgga
     3901 atcagtaggt acatatgtat tattaagaat tataaataat aaaaataaat tttagtcaat
     3961 ctacaaaaac cttttaaatt gtctggaaat cacctcctca tttgttataa aaacatacaa
     4021 caaatgaaga ggtgatttta tggtttttaa aaaaataatt ataagtggat atgtgctttc
     4081 acatgtttat caacatttgt aatgcctata tttgctaatg aattataata taaaaatatt
     4141 aaataatatt caactataga ttatgtaaat tatcctatga aaaaaataag ttgtataaaa
     4201 tctggcaact gtagattgac gatttctttt aaaattacaa ttataataag ttttatagaa
     4261 ttaaaaataa aactttagag atttttgata acattgtaga ctaaatttgc caccgaaatt
     4321 actttacagt ggcaaatcgg tggcaaatac aatatattat tagtatataa tctatataaa
     4381 cattactatt gcttgattat cttagctaca gtttccacat gtgtagaatg agggaacata
     4441 tctacaggtt gtgcctcaac acatttaaat ccgtgaacat ttagatagtc taaatccctt
     4501 gctaaagtag aaggattaca agaaacatag acaattcttt ccggattcat tgaaacaata
     4561 gtctctaata ccttttcgtc acatcccttt ctaggtgggt ctacaactac tacattggca
     4621 gtcttaccct ctctatacat cttaggtaca atttcctctg cctttccaac ataaaattca
     4681 gcattatgta tattgtttaa tttagcattt atcttagcat cttttatggc atcatctata
     4741 atttctattc cataaacctt tttagccttt tgtgccaaaa ataaagatat tgtacctatc
     4801 ccacagtaaa tatcaaaaac agtatcagta tcatttaaat ttgcatattc aagagcctta
     4861 tcatacaata ctttagtttg gacagggttt acttggaaaa atgataatgg tgaaatctca
     4921 aacactaagt cacctatata atcgtttatt tttccatctc catatataac tatattttct
     4981 cttcctagaa tagcattcgt atctttttga tttacattta caaccaaagt cttaaatcca
     5041 ggtatatttt ccttaagcac tgaagctaat tctttaagat atggaagttt gtttccattt
     5101 gctactaata caaccattac ttctttagtt gtaaaaccta tttttgttac taaatgccta
     5161 atcaaccccg tgtgagtctt ttcatcatat atacttacct tataggccct tatatatgtt
     5221 tttattattt taacaatttt atcatttatc tcatgttgta ttacgcattt ttctgtcgat
     5281 attacatcat gagtcttctt tttatagaag ccaataattg gaataccatt attcttttgt
     5341 attggaaatt gtgctttatt tctatatcta tatggattat ccattcctat agtgtcatga
     5401 acttctacat cctctaactt acctattctc gaaagtactt gtttaacttc atttgttttc
     5461 atttcaagtt gtttattata atctaaatct tgtatctgac accctccaca atccctaaga
     5521 ttgtcactac acactctttt caccctgaat ggagactttt ctattatctc aacaatatct
     5581 ccctcagcat aatttttctt tactttatta atcctaactt taactttatc atgtattaat
     5641 ccgccctcta caaaaactgt aataccattg tactttccta ctcctactcc gccttgacca
     5701 atgtctacta tatctacaat atacatctcg tttttcgaaa gcataaatca ccctccaatc
     5761 aatctattat atttaaatct ttacaaactt ttttctttaa atttatatta aaatattgct
     5821 attaattaaa ctatattttt tactaagcta caatatttta ttttaccaaa aaaaaaggag
     5881 cttataaagc cccttagtaa ataaatttat tatttttctt ataaaactct agttgaacct
     5941 ctatatacca cgcctcttga tgcatccaca gttacaactt ctccatcttt tactagattt
     6001 gttatatcag ttgcagacac aataactggt atatctaaat ttatacctac aatagctgta
     6061 tgtgatgtca ttccaccatt ctcagttact acagctcctg ctttttctat ataattgttc
     6121 atttcagcat cagtagcagt agaaacaagt atgtcgcctt cattaaagtc tacacaagca
     6181 tttccattct ttattatacg aactttacct tctaccgttt gagtacctac acctatacct
     6241 tgcacgattt cttcgcttat tacatgaacc tttattaaat tagtagttcc acttactcct
     6301 actggtactc cagcagttat aactactaat tccccattat ctatataatc agcttgtcta
     6361 gctgcctcaa ttgccttttc tataacttca tctgtatttc ctgcaacaga agacttaact
     6421 ggatatactc cccatgtaag agctaattta ttcatagtct tttcattatt tgtagtagct
     6481 attattggag attttggtct aaatttagat accattctag cagtatatcc tgaagaagta
     6541 gatgttatta tagctgatgc atttaaatct actgctgttg tacaagttgc atggcttatt
     6601 gcatctgtta cagtaacatt gttagtacca ttttccttta gtaatctatc ataatctaaa
     6661 gtctcttctg ttctttttgc tattgtagcc atcattttta ctgcctctac aggatatttt
     6721 ccagcagcag tttctccaga taacattatc gcatctgttc catcatatat agcattggca
     6781 acgtctgtaa cttctgctct tgttggtctt ggatttctta tcatagaatc aagcatttga
     6841 gtggcagtaa caactggctt tgctaattca ttacattttt ttatcatcat tttttgaact
     6901 attggcatct cttcagttgg tatctcaaca cctaagtcac ctctagccac cattatacca
     6961 tcagaaactt ttaatatttc atctaggttc tctacacctt cttggttttc tattttagat
     7021 attatttgta tatcagttgc attattgttc tctaatactt ctcttattgc tagtacatca
     7081 gatgcctttc ttacaaaaga agcagctata taatctattc cttgacttat tccaaactct
     7141 atatcactta tatctttagg agttattgct ggtaaattta tcttaacacc aggtacattt
     7201 actcctttat gatttttaac tattcctgaa ttttcaacaa cacaaactat atcttctcca
     7261 tttatttctt ttactcttaa gccaactaaa ccatcatcta ttagtataga atctcctgat
     7321 ttaacgtctt cagcaagacc tttatagctt actgtacaca tttctttagt ccccataata
     7381 tctttcatag ttatagtgaa cttttgccct tcttctaaaa atacctctgg gtcttcaaag
     7441 ttacctgttc taatttctgg acctttagta tctaataata tagcaactgg cttatttaat
     7501 ttttctctaa ctttctttgc catatcaatt ctttctttat gttcttcatg cgaaccgtga
     7561 gaaaagttaa atctacacac atttagacca ttttgcataa gttgagttaa aatttcctca
     7621 ctttgtgatg ctggacctag tgtacaaaca atttttgtct tctttacatt atttaacata
     7681 tttgctcctc ctgagatatt attccacaga tttattttta atactaaaac ttatatagat
     7741 aatatttttg ccatctcata agcttcttta tcaaatactt tcttttgagc taaagcttca
     7801 cttatttcta aatcaattat tttgttttct tttattccta caactctagc tgattttcca
     7861 tctaataata attctactgc tcttactcca agtctacttg ctagtattct atcagataca
     7921 gttgggcttc ctcctctttg gacatgcccc aatacagtga ctcttaaatc tgctcctgac
     7981 tcttttttaa gttccttttc taaatcacta gcacttccga ctccttctgc taaaacgatt
     8041 atactatgtc tcttacctct tttttgagta gtctttaatc ttagagcaac ttcatctact
     8101 gtaatttcta cttctggtac tattatagtt tccgctcctc ctgcaagacc tgcatataaa
     8161 gctaaatctc cacagtgtct tcccataact tctactatgt taactctttc atgagatgat
     8221 gatgtatctc ttatcttacc tatagcatct ataatagtat tcattgcagt atcaaatcct
     8281 atagtataat ctgtatatgc taaatcgtta tctatagttc ctggtatgcc tatagctggg
     8341 aatcctaagt cactaagttt ttgggctcct gcaaatgaac catctccacc aattacaact
     8401 aagcaatcaa ttttatactt tttaagaatt tttactgctt ttaatctacc ttcttctgtt
     8461 ttaaattctt cacatctgga agattttaat atagttcctc ccctgtgtat aatatctcca
     8521 acagaagata agttcatttc agttaaatct tcttctagta atcccttata tcctctattg
     8581 attccatata ctttgcatcc ataatatatt gctgatctta caaccgctct aatcgcagca
     8641 ttcattcctg gagcatctcc tccacttgtt agtaatccta tagttttcat tttttacctc
     8701 cttggatttt atgtcaattt tttattataa tcattgttat tgtattctaa tggacttttt
     8761 ttgtccctat atcacttttt aatcccatca agactaaaaa aattaaatgt ccttaaatgt
     8821 ccattttgtg atattatatc acaaaacgga caatattttt catattatta taatatattt
     8881 agatttttat ataaaaaatc aagcttttac ttttatgtta tctttaggaa gtaactcttc
     8941 taagttattt ataaattcat cacatataca agtcaatgcc ccggtcattt tatatgcttt
     9001 acgtttacca tttatatttt catccatagg gtacaagtat atactgtcat tacctggata
     9061 ttgtttggct atttctacaa tattattagt tgtatcagta tcagacatac tatctatttt
     9121 caaatacaat tttttaccac tttctggctt aatattttct acatttctag ttcttcctga
     9181 attgtagttt ctatctgcaa agtcagtatc atcatttata tcttttatct ctctagctat
     9241 aagttttgca ttttcgcctt cttttatact taatgttcct tttacatata ttatattatc
     9301 ttcattcaaa atgacattat acttttgtaa taattgagga aaaactatta tttctatagc
     9361 accatacaaa tcttctaatt caataaatgc cataatttca tttcgttttg ttgttttaat
     9421 agttttagtt acaatcattc cacccattat aacatctgtt tcattcattt gtaggtatga
     9481 ctcttcttcc tcttttaatg aatttaattt tccattatcc atagatgttt tttcttgtag
     9541 ttctttttca aattgtgata aaggatgtcc acttacatac attcccaaga cttctttttc
     9601 aaggttaagt ctctctcttt cttcatactc ttgcctttta ggtaaactgt atatttgttg
     9661 aaaatcacta tcttcctcac tatctccaaa agcatcaaat aaagaaattt gaccttgaac
     9721 atttttcttt ctatccatag atacactttc taataaactt tcataaccag ccatcagact
     9781 agctctattt tcacttatat tatcaaaagc tccacatttt attagacttt ctattactct
     9841 cttatttgta tctttttggt ctaatctttt gactaaatct actaaatcta caaataatcc
     9901 attagattct ctttcagcga ttatattgtt tattatattt acaccaacat tttttacagc
     9961 tgctagacca aatcttatat tgtcgccttc aactgaaaac tttgaaaaac ttttgtttat
    10021 atctggaggt aatacatcta tttctagtgc tttacattct cttatatact caactacctt
    10081 atcagtattt cccattacac tggttatcaa tgctgccata aattcaactg gataatatgt
    10141 ttttagataa gctgtctcat atgctaaaac cccatatgca gctgcatgtg atttattaaa
    10201 tgcatatcta gcgaagtcta tcatatcatc aaatatttta ttagctattt cctcttctac
    10261 accatttctg atacaccctg caatttctat attaccatct tcatcaaatt taccatgaat
    10321 aaaatattgt ctttcttctt ccatcacgtc catctttttc ttactcatgg ctcttctaac
    10381 taggtcactt cgtccgtaac tataaccacc taaatctcta acgacctgca taacctgctc
    10441 ttgatatacc aaacaaccat atgtaacttc cattataggc tttaattttt catgtagata
    10501 tgttacatct tttgggttgc ttttattttt tatatatgca ggtatagaat ccattggacc
    10561 tggtctaaat agggatattc ccgctacaat atcttcaaaa ttgtctggtt ttagctgttt
    10621 cataaaactt ctcatgccag cactctccaa ttgaaacacc cctagtgtat ttcctgatga
    10681 aagcatttca tatactttcg ggtcgtcata atccatcttt gaaaagtcta ttttttctgt
    10741 gtagtttttt ataactctat ttttttctat taaatcaaga gtatctctta taactgtaag
    10801 agttcttagc ccaagaaaat ccatctttaa aagcccaagt tcttcaagtg ttgtcatagt
    10861 aaactgtgtt gtgattgcat cttgatgttt gtacaaaggt acatattcat ctacgggatt
    10921 ttttgatata acaactcctg ctgcatgagt agaagcatgt cttagcattc cctctatctg
    10981 ttttgatata tttattattt cttttgtttc cgggtcttgg tcatacaatt ctttaagatt
    11041 aggatttgta tctagggcct tatctattgt cattcctaaa gcaaatggta tttccttggc
    11101 taccttgtca actttattgt agcctatatc caataccctt ccaacatctc ttattgcagc
    11161 ttttgcaccc attgttccaa aagttattat ctgggctaca tggtcatcac catattttct
    11221 ctttacataa tctataactt cttctcttct ctcataacaa aaatctatat ctatatctgg
    11281 catggatata cgttctggat ttaaaaaacg ctcaaataat agagagtact ttattgggtc
    11341 tatatcagtt atcttaagtg tatatgctac tatcgaacca gccgcactac ctctacctgg
    11401 tcctactata atattatttt ctttagaaaa atttataaaa tcccaaacaa ttaaaaaata
    11461 ctctacataa cccatttttt caattacatc taattcataa tttaatctct ctagtatctc
    11521 attgctagga ttatcatatc tctcttttaa tccattaaaa cacaactccc ttaaataagt
    11581 ttctggtgta tatccttctg gaacatcata cttaggaaga tgtatagtat taaaatcaaa
    11641 ctccacatta cacatatcag ctattttcac agtattgtca atagcctcta atgcgtatgg
    11701 aaataattct tccatttctt ctcttgattt taagtaaaac tcatcactac caaatctcat
    11761 tctatttggg tcatttaatg tttttcccat ttgaatacac ataagaacat catgtatttt
    11821 cgaatcttct ttatttacat agtgaacatc attagtagca actagtggta taccagtctc
    11881 ttttgataat tttactaaac cagagtttac ttctttttgc tcaggtaaat tgtgatcttg
    11941 aatctccaaa aagaaatttt cttctccaaa tatgtctctg tagtcaagag caaattgttt
    12001 tgctttttca taatttctat tcattaaagc acgtgctaca tctcctgcaa ggcatgcaga
    12061 aagtgctata atccctttgt tatgtttttt aagttcttcc atgtcaactc taggcttata
    12121 gtaaaaacct tctacgtatg aagttgaaac tatttttatt aaatttttat atccttccat
    12181 attcttagct aacagaacta aatgtccttg atatttgtca tagtttgggt ctttatctct
    12241 caatccacgt gcagctgtat agacttcgca ccctataatt ggctttattc cttcttttat
    12301 agctgttttg tagaaatcta taacaccaaa catacaacca tggtcagtta tagctattga
    12361 gctcatgttt agttccttag ccctttttat aagttttttc accctcgaaa acccatcaag
    12421 taaactatac tcagtatgta catgaagatg agcaaaatct ttgttcatta aatcacctcc
    12481 tgctttataa ttttaatttt accacaaata tatgtcctaa aacaaaaaca gagactttta
    12541 tctcaaaata ggaattaact tcaacatata ccattatatg ttataaatta atgcttattc
    12601 tgagattgtc ccctcaattt attgctatga tttttttttc aaatctgttg caatttcttc
    12661 tatttttcta agtctatgat tgactccaga ttttcctatt ggtggttcta acatctctcc
    12721 aagttctttg agagtcatat cctcatactc gattctaatc ttagctatct cttgtaaatt
    12781 ttctggcaaa ctgctaattc ctatagtctc ttgtatgagc ttaatatttt ctacttgtct
    12841 tactgctgca tttactgttt tagaaaggtt agctgtctca caatttacta tcctgtttac
    12901 attatttctc atttctttta ctattttagt attttgaaga cttaaaagag cgtggtgtcc
    12961 tcctataata ctcaacaaat ctgatatttg ttcactctct ttgatataaa caacgtgatt
    13021 attttttcta gccacaattt tactattaaa acctaaagag tttataagtt cttttagaga
    13081 atctgcaaac tcctcattat tagttacaaa ttccatatga tagttctttt ctgggtcgct
    13141 tatagaacct ccaccaagaa aagctcctct tatgaaagct ctcttacatc cttcatgctt
    13201 aattaagttc tctgaaattt tgtttatagt aaaaaagcct tctttatctt caagtatacc
    13261 taactttttt aataacattt ctgaacccat ttcgcttgtt accattaata cataactgtt
    13321 atttctcttt agcatttgat tcttatttac tgagattgta gtattgatat taaaattctt
    13381 ttttaataat ttaaatactt tacgggctat tgaatttaac tctgttgtta tctttagatt
    13441 tagcttctta tatccaacga tttgtatact tccccctgat ttaactagag ctgataattc
    13501 agctatatta caacattcat tcccagatat aactcttgct agttcatttt tggtttcagt
    13561 tgaaaaagac atatctatca cctattaact aatttttctt taaccttttt tttatttctt
    13621 gtaaggcaac ctttattatc tccttttcat catcatacct agctaggtct accactctat
    13681 ctagatggat aaatttagca tcaataaagc cttcctcttt ttgtggaata gtatccatat
    13741 tctttgcttg catcaaatac caaaaaactg ttttatgaac tgctctattt tcatcccagt
    13801 tttctttgaa tgtatagtgt atttcaccta aatatttcaa tatatctgct tttactccag
    13861 tttcttcact tacttcacgt aatgctgcct cttggttatt ttccccttct tctacttttc
    13921 ccttgggtaa cacccaatct ccattgaact tccttagtaa aagaatcgta ttgccaaaca
    13981 ggactacacc accagcacta acctcttctc tcatcgatgt tcactcctta aaactaacat
    14041 aatgataagg atttctacac aattttttaa aatccttttt tactaacttt tgttgtaatt
    14101 atgatttagg gctaactcaa taactatgtt agatatatac tttgcatcat gtcttatata
    14161 attatttttt atttcaatta aattcttctc tacggttttt atacccatct cttttaattt
    14221 atctttttgc tttttatcta acaagacttg ctctgctcca tcttgtttat ataagtcaag
    14281 catttcttct ggcaatatct cattatttgc gattacataa tctattaaat tctcatcagt
    14341 gtgctttact atagcattaa catgctctaa tacattgtac ccttctgtct cacctggttg
    14401 agtcattata tttgctatat atacttttgg agctacagac gatttaatag cgtctacaat
    14461 cccctctaca agtaaattgg gtattatact cgtatataag cttccagggc ccataattat
    14521 aatgtcagca tcgtaaatag atgcaataac atcctttaat ggtttgacat cttttggatt
    14581 taaaaaaatt ttatcaatag agcttttttg aatttttgac tcttcaggta tactagattc
    14641 tccatttatt atattgccat tttctaattt ggcaactaaa tttacatctt ccaaagttac
    14701 tggtaaaact cttcctgtta tagcaaaaat ttcacttaat ttatatacag ctttttcaaa
    14761 gtttccatac aacccattca tagcagccaa aaacaaattt ccaaaactct gaccttttaa
    14821 aagcccttct gtaaacctat attgcataac ttcattcatt gttggctcta tatttgcaag
    14881 tgctaataga cagtttctta tgtctcctgg tgggagcatt cctaagtctt ctctcaatac
    14941 acctgaacct ccaccatcat cagcaacagt tacaattgca gttatatttt gcgtagtgtg
    15001 tttaagacct cttaaaaata ctgattgacc agtacctcca cctatcacaa ccacatttgg
    15061 ttctctattt tcaacaatag acctttgaaa tagttttact ttttcatttt ttgctttttt
    15121 atatataaat aacgatacaa ttaatgcaat ccaacctaaa attcctacaa taataagtaa
    15181 tttagccatg tttgcctcca ttatcgcaac atagagtctc tatgtttctt tacaactcta
    15241 tatcccttat tagataaatc ctccgctata agatttgtta tagtaactga cctatgtctt
    15301 ccacctgtgc atccaacacc aattactaga tgttgttttc cttcatctat atattgaggt
    15361 actaaaaaat gaatcatatc taaaagtttc acataaaatt cttcactgat tttagaattc
    15421 ataacataat ctctaacttc tttatcatca ccagttttag ctcttaactc ttcaacataa
    15481 tagggatttg gaagaaatct tacatcaaat actaaatctg catcagcaag tataccatgt
    15541 ttaaatccaa aagatactac agaaatagtt aggtttggat tatcttcacc agaagaatat
    15601 attttcttta tttcttcctt aaggtcttta ggtttcatgt tagttgtatc aattatacat
    15661 gtagataact cctttaaagg ttccattatc tttctttcca tttttatacc ttctggaatc
    15721 tgcatatctt ttgccaatgg atgatttctt cttgtcattt tatatctctt taacaataca
    15781 tcatcattac agtccaaata taccatttca tattcataat tttctttttc taaataattt
    15841 aagctttcat gaagcgcttc aaaaaacttt ctccctctta tatctatccc tagagcaact
    15901 ttatcaatac ttgaatttgg ttgataacat aattctgcaa atttagttat caaagcagga
    15961 ggtagattat ctacacaata aaatcccata tcctctaaag ctctcattgt ctcacttttt
    16021 ccagatcctg aaagacctgt aactataaca aatttcattg ctaactcctc ccttttatct
    16081 tatgtttatt atattataat gttttctact aacgatatat ccagattaga gtcagatata
    16141 gtcttcatag ctagtttaaa atcaccaact ctttctggac aatgtgcatc tgagcctatt
    16201 ataaatttat tttctagccc ttttatttta tttaattgat ttacattcaa aaaagaatgg
    16261 ctactattta tttcaagtct agtatttgtc ttttttgctg tctttgctac ttcttctata
    16321 tacacatcgc ctttatctcc tggatgagta attataaaaa tatcattatt acttatcgca
    16381 tttattacag cttttgtatt gtattctttt gctttttcag ttttaaaaat atagttatta
    16441 caatgattta ataagcttct taatgatgtt ggagtagaac caaaatgata tccagccata
    16501 atataatcaa aatctctaat aaatttatca tccatatcta tattgccttt atcatctaat
    16561 atgttacact ccatacctaa aagtatattt atatcattgt acttatcatt taaataatct
    16621 acttcttctc tcattttgag aatatctttt ttctttacac catatgcaat atgtttatag
    16681 ctatggtcag atattcctat tgtttttatt cctttagaaa tggctacctt tacattatct
    16741 tcaatagtcc ctttgccatg actatataat gtatgtgtat gataatcagc cagtattttc
    16801 atttgttaat cctctccaag tattttgact tcttcttcca acataacccc aaacttggca
    16861 tatacagcac tttttataac atatattaaa tccaaaatat ctttagcact tgcttctcct
    16921 tgattgacta caaatccaca atgcttctca gatacttgtg ctcctcttaa agtaagacct
    16981 ctcaatccag tttcttctat taatttacct gcaaaatatc ctgttggtct cttaaatgta
    17041 cttcctgcac tcgcaaaatt taatggctgt ttagttattc ttcttgtcgc taaatcatcc
    17101 attaaacttt ttatttcatc ataatttcct tcttgtaatt ctagttctgc tgataaagct
    17161 atatatccgt ttttcgatat tatacttttt ctatacccaa attccatctg ttcatttgat
    17221 aattcaaata tattgccttc catatccatc agtcttactg acttcactat gtccttcatt
    17281 tcaccaccat atgctccagc attcatggct agtgctcctc ccaatgtacc aggtattcct
    17341 gatgcaaact caaacccttt taattcttgc ttttggagag ctttacctaa aactgataat
    17401 aaagctcctg actgagcagt catcctagta tcatttatct cataagaatt gaaattatca
    17461 gctaattcta ttaccactcc tcttattcca ccatctttaa ttaaaatgtt tgaaccattt
    17521 ccaattatta aatacggaat agattcttct tttaccaact taagtacatt ttttaattgt
    17581 tcttcagttc taggtctaac taatatatct gctggtcctc ctaccctaaa tgaaatatgt
    17641 tttttcatag gttcatcgac ttttatatcc tctttactta atatatttaa taaatttata
    17701 tatatatctt gattattcat tgtatttcct cccaaattat actgaacttt agctgtcatt
    17761 attatttaaa ttacattagt atatagtatt aactatattc aataatatat gattttgctt
    17821 atcatatgga ttttatattt taagcttctt aattacaaac tatattcaat aaatatataa
    17881 taaagtaaaa aaaaaaaaat aacaacatat aaaagaatat aaataattta aaaaaagcat
    17941 caatatcact gatatcaatg ctttaaaagg caatttaatt cgtattattt atctaactat
    18001 ttttgacata gcaaagataa taaatccctc caactactat ggaacctcca acgatatttc
    18061 caagcgttac aaataccaaa ttagttataa attgaaccat agttatattt gctcctaatg
    18121 ccattcccat tggtataaaa aacatgtttg caacactatg ttggaatccg cataatacaa
    18181 ataacattat tactgtccaa catgaagcca attttgacat tatatcttga gatgctgtag
    18241 ccatccatac agctaaaacg actaaaatat tacaaagtat tgcttttaaa aacattgatg
    18301 ctatagttga attaacctta gcttctgcca cagctatagc ttttgtagct gcatctcctg
    18361 tcaatgtaga agaataaaac acaagtgcaa ctagcagaat tgaacctact aaatttgcaa
    18421 aataaaccag tccccagttt cttaacattt ttcctaatgt tatttttttg tccataagtg
    18481 caatagccat taaggtgttt ccagtgaata attctgctcc tgccaccact acaagcataa
    18541 gacctactgg aaagaccata gcacttaaaa agctagctac ccctgggtct atcttgccaa
    18601 aagtttgact aactatcata ctccccaatg ccccaaaacc tataaatgct cctccaaata
    18661 aacctaatag aattgcattt tgtgtcgaaa tgtttgcttt tttaatgcct gcacttatag
    18721 ttgcttgtgc agtttcccca ggtgtcaaga atcctttact cataaatcct tcccccttaa
    18781 aaattatact ttcccttgtc tttttatggc aataattata taataacaat atgatatata
    18841 ataaatttta ttaacataaa aaatatacta tatacttata tatacttgtc tcatataatt
    18901 atactattta ttatataaca actttgttaa tttcgaaata atttatattg aatttgaaaa
    18961 atttaccttt tgattattta ttttactttt taattattga ggtattatat ataatagata
    19021 tatgtttgtg ttattatata taattactta ttttaataaa tatttgaagg agggaatttt
    19081 atgtttgata tatcattttt cgaaatgtca atatatgaaa tagcagttac tacaatatat
    19141 ataattaatt tcatggttat actaaacctt atctttagag aaaaaagaaa cataaataca
    19201 actttaacat ggcttcttat attagtattg attcctgctc ttggattcat actttatata
    19261 gcttttggca gaaatatttc aaaaaacaat atgtttagat tgaaagaaaa ggatgataag
    19321 ataatcaaaa gtaatatact agatactcag gttaaactcc aatctacatc tgaaattgat
    19381 tcagatatac atcaacataa ggatatgatt tatgcattgg ctaattcaaa taatgctcat
    19441 tataccaata ataacgatgt gtggatttat gctgaatcta gtcaattttt taacagtctt
    19501 cttgaagaac ttaaaaaagc taaaaaatat ataaacatac aattttacat tttcaaagat
    19561 gacaaaatcg gaactgaaat aatagacatt cttatagaca aagctaaaga aggtgttgaa
    19621 gtaagacttt tatttgatgc agttggtggt agaacactaa aaaatagtac actttccaga
    19681 cttaaagaaa gtggtgttaa agttggaagc ttcttcccat cattcttgaa aattgtaaac
    19741 tttaacttaa actatagaaa tcaccgaaaa attgttgtca ttgatggtaa ggtaggattt
    19801 gtaggtggat ataatgttgg tgatgaatat ttaggtagaa atccaaaatt tggtctatgg
    19861 agagatacac atactaagct tactggtgat tgcgtaattg acctaaatat gagattcatc
    19921 cttgattgga gatatacaac taaagaagat ttagatttag aaaagtactt tatagaagaa
    19981 gatacaaatt ctaatcatcc ttctgaaaat attgggattc agatagtatc aagtggtcca
    20041 gatatatcag aacttgatga gattaaatat ggttacctta aaatgattca gaaagctaga
    20101 aagtatatat acatacagag tccttattta atacttgact caacttttat agatacttta
    20161 aaaatagctt gtttatctgg tgttgatgtg agagttatga ttccttctaa acctgaccat
    20221 ccctttgtat actgggcatc atattcatat gctggagaac ttttaaagtt tggagctaaa
    20281 atctacactt atggtcaaaa tgcattttta catgctaaga caatagttat agatgactct
    20341 atatgctcta taggaacagc taatatggat atcagaagtt ttgagcttaa tttcgaagtt
    20401 aatgcattta tgtactcttc aaaaaaagca ttggaacaaa gaattatatt tgaaaatgat
    20461 attttaaatt ctaaggaaat aacattagat atatataatt ctaggtctac ttatataaaa
    20521 atcaaggaat caatttcaag actattatca gcagttttat aaatctttta ttttataaaa
    20581 attttacatt ttggtttatt ttttaaagtt ttttatatgt attatttttt ttttattgaa
    20641 tttagctcat tttttttatt ttttttaact attctgaatt attttatatt ataatagact
    20701 agtatagttt ttatttttaa taggtaactt ttttgcattg tttttataaa taaagaaatt
    20761 gttgtatatt attctttctt tattgttctg gttttgttca cccacataat gttggggtca
    20821 actcatggtt aattatttta tgaaaaataa taactttaaa aagaaaaggg ggatttgtta
    20881 tgtgcgattt tatttcacac aacaagcaat tatttgacga actagatata tttattgact
    20941 ctcttacaac aaaggaaggt gcgttaatac aagttctaca tgaagcccaa ggtatatttg
    21001 gatatcttcc aaaagaagtt caacttcatg ttgctagaaa acttggagta gctcctgcaa
    21061 aggtatatgg ggttgttaca ttctattctt attttactac agaacctgta ggtaagtata
    21121 aaatcagtgt ctgtctagga actgtatgtt ttgtaaaagg tgctgacaaa atcttaagtg
    21181 cttttgaaaa acagcttggc ataaaagttg gagaaactac tagtgatttt aaattttctt
    21241 tagagggttt aagatgtttg ggggcatgtg gtttagctcc agttgttact gtaaatggta
    21301 aagtttatgg taaagtgaaa cctgaccaag taagcgaaat attagatact tatagagaat
    21361 tagaattgaa ttgttaagag gggtgaataa ataatgcgta aagtaaattc atttgatgaa
    21421 ttaaaagttt tagctgatga gctgaaacct aatcttagct taagaaaaaa ttattctaag
    21481 gaaaatgtat taagaagaga attacttgtt tgttgtgata ctggatgtac ctcttcaaac
    21541 agtctagaaa tagttagtga attagaaaat gaaataaaga aatcaggaat ccaagataaa
    21601 gttagtgttc gtttgacagg ttgttttggt ttctgtgctc aaggtcctat tgtaaaagtt
    21661 tatcctgata acgttttcta cgtaaaagtt gagccatctg atgctgaaaa gatagttcaa
    21721 agccacttaa ttagaaatac agttgtcgag gaattattat atgaggaaga atctttaagt
    21781 aaaaaagttg aagaacaaga agaaatgtct ttctacaaaa aacagttacg tatagcttta
    21841 aaaaattgtg gtcttataga cccagatagt atagaagaat atattgccaa tgatggttat
    21901 ttagctctag gcaaatgttt aacttctctt actcctcaag aagttattgc agaggtaaaa
    21961 acttctggcc ttcgtggtag aggtggtgca ggtttcccta ctggctctaa atgggaagct
    22021 gcttcaaaaa atccagctgg tccaacagat aaaaagtttg ttgtatgtaa tgctgatgaa
    22081 ggtgacccag gtgcatttat ggatcgatct gtacttgaag gtgaccctca tagtgtacta
    22141 gaagctatgg caatatgtgg atacgctata ggttctgata caggatacat atacataaga
    22201 gcagaatatc caaaatctat agaaagatta aaagtagcca tagctcaagc tgaaaaatac
    22261 ggtttacttg gcgaaaatat cttaggcact ggatttaact ttaaattaga attaaaatat
    22321 ggtgcaggtg ctttcgtttg tggtgaaggt acagcgctaa tgcattcaat tgaaggtaga
    22381 cgtggtgaac ctagaatgaa aacttatagt tcttctaaga gtggtctatg gaaatctcct
    22441 acttgcttaa ataacgttga gacttttgct aacatacctg caattatact taagggtgga
    22501 gattggtttg ccaacttagg tactgaagat tctagtggta ctaaagtctt tgctcttggt
    22561 ggaaaagtag aaaatgtagg acttgttgaa gttccaatgg gtactacttt aagagatatt
    22621 gtatttgaaa ttggtggggg aatccctgaa ggtaagaaat ttaaggctgt tcaaacaggt
    22681 ggaccatctg gtggatgtat accaactgaa catttagaca ctcctataga ctttggttca
    22741 ttaagttcta tcggttcaat gatgggctct ggtggtatgc ttgttcttga tgaatctgac
    22801 tgtatggtag atatagccaa gttcttcctt gagtttactg ttgaagaatc ttgtggtaaa
    22861 tgtacacctt gtcgtatagg tactacaaga ttattagaaa tattaactaa aattacagaa
    22921 ggtaatggta ctctacaaga tttagatgac ctagaaaacc ttgctgaaac tatacaaact
    22981 gcttcattat gtggtcttgg taaagctgct ccaaaccctg ttttaagtac tcttcaatat
    23041 ttcaaagatg agtatatagc tcatgttgta gataagaaat gtcctgctgg taaatgtcag
    23101 aacttacttt cttactttat aacagatgac tgtaagggtt gtacaaaatg ttctagagtg
    23161 tgtcctgctg gttgtataac aggttctgtt aaggaacaac atacaataga tacttctaag
    23221 tgtctaaaat gtggtgcttg tatagataat tgtacattta acgctataat caagaaataa
    23281 cttatttgga ggaggaataa caatgagttt agttaattta actattaatg gcaaacacgt
    23341 ttcagcacct agtggaactt ctatcttaga tgctgcgaaa ttaataaata taaaaataca
    23401 caacctttgt catttacata tgaacgaaat agataagctt gacacttgtg cttcttgtcg
    23461 tatttgtatg gttgagacag agagaggctt agttccagct tgtggaacag ttataaagga
    23521 aggtatgaaa gttcagacta atagtgctaa agcattaaat gctcgtagaa ctattgttga
    23581 attattgtta tcagaccatc cacaagattg ttttgtctgt gaaaaaaatg gagattgtga
    23641 attacagact atagctgctg atttaggagt aagaaaaata agatatcaag gttccaaatc
    23701 ttttgcaggt aaagatactt ctactaaatc tttagttaaa gaccattcta agtgtatatt
    23761 atgtagaaga tgtgagactg tatgtaatga catacaaaca gtaggagcat tatctggagt
    23821 taatcgtgga tttaatactt tagttagtac attctttaac tctgatatgg ttgaaacaga
    23881 gtgtacattc tgtggtcaat gtatatctgt ctgtccaact ggagcattaa cagaagtaga
    23941 taatgtacct aaactttggg atgttttaaa taaaaaagaa aagactatag tagtgcaagt
    24001 tgctcctgct gtaagagttg ctattggtga agagtttgga ttagaaccag ggtcaatctc
    24061 tacaggaaaa atggtagctg ctttaaaagc acttggattt gaacatgtat ttgatacaaa
    24121 ctttggtgca gattttacta taatggaaga agctactgaa tttatagaaa gaatccaaaa
    24181 aggtgaaaat cttcctatat taacaagttg ttgtcctgca tgggtaaact tcttagaaca
    24241 caattatcca gacaagttaa acttagcatc tagctgtaag tctcctcaag gtatgtttgg
    24301 ttctatagct aaaaactact atgcacctaa aatattagga atcaatcctg aagaacttta
    24361 tgttgtatct gtaatgcctt gtgtggctaa aaaatatgaa gcttcaagag aagaattaag
    24421 tgaatctgga atactagatg ttgacctttc tataactact agagagttag ctaaaatgat
    24481 aaaagaagct gctatcgatt tacctaatct tcaagaccaa gattttgata atcctctagg
    24541 aaaatctaca ggtgctgcat ctatatttgg agcaagtggt ggagttttag aagctgccct
    24601 acgtacttca tatgagaaga taactaataa gactttagat aatgtaaact tcactaatgt
    24661 tagaggtctt aaaggaatta gggaagcaag tatagatgtt gatggaacta cagttaatgt
    24721 gtgtattgta aatacactta aaaatgctag aaagattatg gataaggtca gaagtggaga
    24781 atgtaaatac catatcatag aagttatggc atgtcctggt ggatgtgttg gaggtgctgg
    24841 tcaaccttat caccatggta atactgaaat agttgataga cgtgcaaatg cgttatatga
    24901 aatagataga aataaggcta ttcgtaaatc tcatgagaat cctgatttac aagctattta
    24961 taaagacttc tttggagaac caaatagtga tgtagcccat aaataccttc atactcatta
    25021 ctttgataag agttgtgtct atggagagtg tccacaagaa tgtgcttgtg aagaagctaa
    25081 gtagtcataa caaagtataa aatttattct taaaaaagga ttgtcttaaa atacttttac
    25141 gtaatttaag acaatcctta ttaatttcac aaattaaatt atatttctaa atagacttat
    25201 tctttaatta acctatctat taattaaaga atacctgcca ttctcataag ttgttttgca
    25261 tttgtagttt tacatcttcc atcttttatc ttatagtcaa aatgaatttt atcgtctaca
    25321 tagtactctg agaaatgata gtttacaact tcaacatctc cactacctga aaggtcacat
    25381 agctcaaagt catgtgttgt caccatactt ataacccagg gttttgataa tttctttact
    25441 gcctcacttg ctccaataat tctatcagca gagttagttc ccttaaaaat ttcatctact
    25501 agaaccaaca ttggcagttc ttttaaacta tattgaatca ttgattttat tctaagaatc
    25561 tcagcataga aagttgatat tccttgactt acatcatctt gtattctcat agaagtaaat
    25621 atagccatac aggtcgcatc aaaactctta gcgcatactg gagcacctgc atatgccagc
    25681 actaagttta caccgatact tcttaaaaat gttgtctttc cagacatatt tgaacctgtg
    25741 ataatacaag tctgagaatt taattgtatc gaatttgcta tgacactttt ttctgctatt
    25801 aatgggtggt atacctcttc aactttcaat cttggagtat cttcatattt tattactgga
    25861 aaacatgtat tttcctttac atgagaaata actgttaaac ttattagagc ttcaaattct
    25921 ccaatagatt caaaccaatc tctaatttga tttccatata tacctttcca tcgctccaaa
    25981 gcttctgcac aatgatagtt ccacatcaaa acaccacagg caattatata tagtatctga
    26041 ttatacctta ggtttacagc atttcctatt gaatttaatt gtttaacacc tcttgaaact
    26101 ccacttccct tagaaagttt atcttgtaat tctttcaaat atgcactctc aaacgtctct
    26161 ttttctaaaa cctcaaacat tttttcatat acttgtatac ttctactaaa tgaaaataat
    26221 ggagaaagag tttgcataag tcttggatat ccaaaaccac taaaccctaa ctggattata
    26281 aaaagtaaaa aaacaggtaa aacaggtaaa aatcctaaca tacaaaatat aaaagataag
    26341 attgttgcag ttggtagacc ccaagtaaaa atatgcatcc attttggtat atacacattc
    26401 ttattttctc catactctat aaacgattct atactactat ctgaattatt tttttgctct
    26461 ttaccaatta tattactgag tatttgaata tgaatagaaa aatcatcttt tgataaaagt
    26521 tcttttatag ccctctgtct ttctaagatt acattctcat ctggattttc attccacaaa
    26581 tatttggcta aaaaattttt cccataagat gtattagcta cacagatata ttgatacaat
    26641 gatgctcttc caaataagtc taaatctttt gcttgcgaat tttcatcttt taagtattct
    26701 ttaccatcta ttttaaattc cttccaacca tctccaaatc tcttaatata cttatttaat
    26761 acttcgctct tacttttaaa atatgtttct tcttctttta ctttactatg aataactaag
    26821 agtgctataa atattattat tgataaaact ccaacaaaaa tacctactat atttttattg
    26881 taaaatccaa tagcaaaccc tgctatcact atcaaaaaag atattaatct tgctgtactt
    26941 atgttatttg actttttact taagagcaat tctttttcag tactactttc taaaagttta
    27001 ctaaaatatt ctattcccat aaaaaattcc aactttcttg ttatataatg aaactcaaat
    27061 ataaaattaa tctagtatta ttataatcta acactgtact ttaaattatc cttatataat
    27121 atatcttatt aataaacatt atatatatac aacttgattt taatttataa ggtgtaggac
    27181 ataatcctaa tcaatattta attaaatgat gtgtagttca ctattattca gtactactta
    27241 gtatcgatta attttgataa ttttttagta aattcttgag ctgcatcgta tcccattcct
    27301 ctttgtctaa aattcattgc tgctacttct aatatcatag cagtatttct tccaggttta
    27361 acaggtacaa ctaatttctc tacattaaca ccaagtattt cttcgtattc tttatccagt
    27421 cctagtctat cataatactt agtttcatcc caagcttcta aatgaactac taaatctatc
    27481 attttagaat ttttgattgc ccctactcca tataaacttc taacatctat tattcctatt
    27541 cctcttatct caagaaaatg tcttattagt tcaggtgact gacccacaag catactttca
    27601 tctaattttc ttatttctac agcatcatct gcaactaatc tattacccct ttgaatcagt
    27661 tctaatgctg tttccgactt tcctatacta ctttcacctt ttatcagtac acctatacca
    27721 taaatatcta ctagcacccc gtgaattgtt gtgtgtggtg ctaacttatt atccaaatag
    27781 tttgacagtc tatttataag tctagtggta tttctatttg tgctcaaaag tattctttta
    27841 tattttttag ccttttcaat tacatctggc ttgatttcca agtctctaga aattattaac
    27901 gctggtattt catatgagaa aaatctatct agtacttctt ctcgaatatg actatccatt
    27961 aatccaaaaa aattatattc tgtctttcca actatttgta ttctttcata tgcaaaataa
    28021 tcaaaatatc ctgcaaactg taatcctggt ctatttatat cttgagagta gacataataa
    28081 tctacatcat caggcatgta tactactttt aacttcaact cttttattat ctctcttatt
    28141 gatactttat tgctagtttc cattaatttt cttcctttct aaaaaaatta taaacattat
    28201 ctgcaatgct tttattcatt ccatctactt ctaaaagttc ttccatagta gcttttttta
    28261 tagcctctac atctttaaag tgatttaata atgatttctt tcttttttca cctattcctt
    28321 gtatatcatc taagatagat tttgtcaaag ctttatttct tagacttcta tgatatgtta
    28381 ttgcataatt atgaacttct tcttgtatac tagcaataaa tctatataaa ttagttgttt
    28441 tatctagttc tatttctttt tctttgcata tcaatccttt agttctatgc ttatcatctt
    28501 tgtacattcc ccaaagaggt atatctacat cattgagttc tagtactttt tttactgcac
    28561 taacttgtcc ttttccacca tccaacaata tcaagtcagg taaatttcca tgttttaatc
    28621 ttctatctac aatttcagcc atagaatcat aatcattagg tccaataact gtctttattt
    28681 tatatctcct atattccttc ttatcttttt tagcattcgt atacactacc atagaaccaa
    28741 tcgaatctac accttgtata tttgatatat cataagcctc tattcttatt ggcaactttt
    28801 ctagatttaa tatttgtttt aattcttcca atgctccaat acttttttca tattttcttt
    28861 tattcatatc agaaaatttt tcaagatatt ctacagcatt tttcctaacc atttcaacta
    28921 agcttttctt ctctcccttt tgaggaactc ttattgttac tttttgcccc tttttagatg
    28981 acagccattc ttctaatata gaactatctt ctatctcatc ttctataatt aattctttag
    29041 gtatatactc ttgttcatta taaaattgtt ttacaaatga acttaatata gaagctcttg
    29101 gactatccat tacaccttct aatataaaat gttccctacc tacaatctta ccatttctta
    29161 taaaaaatac ctggacacaa gcttcattat gagctcttgc cattgcaacc acatcttggt
    29221 ttaagtcact tacagtagca tctatttttt gtttctgtat catctcttca aggctcttta
    29281 tcttatccct atatacagca gcctcttcaa atctaaagtt catagaactc tcattcattt
    29341 tttcctttaa aagttccatc aatttttctt ctttaccaga taaacacatt ataatctctt
    29401 ctatcatttt tccatactct tctttagaaa catttcctgt acaaggtcct aaacatttat
    29461 ttatgtgtag atttaaacaa ggtcttgtct tattctttat tgctttatct atatctattt
    29521 tacagcttct tattggatac gtactactta taagctccaa ggtatcattc acagcagtta
    29581 tattggtata gggtccaaaa tactttgcct tatctttaag aactcttcta acttttaaga
    29641 ttcttggata atcttcattc gtagttactt ttatatatgg ataagtttta tcatctctaa
    29701 gcactacatt atactttggt ctgtatcttt ttattaaatt acattcaagt attaatgcct
    29761 ctaactctga atccgttata atatactcaa acttatatat attcttaacc atagatttta
    29821 ctttagatgt atggtttttt gaagattgaa aatactgcct taccctattt tttagagata
    29881 tggcttttcc cacatatatt atatggtcgt atttgtcttt cattaaatat actccgggct
    29941 cagaaggtaa tttttttaaa tgctcttgta tatcaaacat ctcgccctcc aaaacattaa
    30001 attttatact aaaattatac cctaaaatat gaattttaaa aagttataca atttccagtg
    30061 ctttagacaa gttaaagtcc atattccctt ctataattga tttgaaaaca attttaacca
    30121 tgaagagtaa tatggacttt actttgtatt actatttaca acttatattt gactaattta
    30181 cttataacaa aacagtttat cgtatatctg ctctaaaatt tttcacaatt tatataactt
    30241 aagaaaaata tttctttaag aattgacctg tataagaacc atcaacttta gatacttctt
    30301 ttggtgttcc agttgcaact atagttccac ccttatctcc gccttctggt cctaagtcta
    30361 ttacataatc acaagtcttt ataacatcta aattatgttc tatgactata attgtatttc
    30421 ctgtatctgc tagtctttgt aatacttgga ttagcttgtc tacatctgcc atatgaagtc
    30481 cagtagtagg ttcatctaat atatacaaag tctttcctgt tggtctttta ctaagttcag
    30541 cagctaattt tatacgttgc gcttctcctc ctgataattg agttgaaggc tgtcctaatt
    30601 taatatatga aagtcctaca tccattaaag tttctaattt tctttttata tttggtatat
    30661 tttcgaaaaa ctcaacagct tcttccacat tcatatctaa tatatcagat atagttttat
    30721 ctttgtattt tacttgcaat gtttctctat tgtatctttc acctttacaa atttcacaag
    30781 gaacatatac atctggtaag aaatgcattt ctatttttat tattccatca cccttacaag
    30841 cttcacatct accacctttt acattaaaac taaatctacc ttttttataa cctcttgctt
    30901 tagcttcatt agtagatgca aaaatatctc ttataaaatc aaatacacct gtataagtag
    30961 caggatttga acgaggtgtt cttccaatag gactttggtc tatatttata actttatcta
    31021 tactttcaat tccttttatc tccttatgct taccagctct ttgcttaagt ctattcactt
    31081 tacttgcaac acctttgtaa agtatatcgt ttattaatgt actttttcca gaacctgaaa
    31141 cacctgttat gcatgtaaac ttacctaatg ggaactttac atcaatattt ttcagattat
    31201 tttctgatgc tttaactatt tctataaaac ttccatttcc ttctctagta gtctcaggaa
    31261 gcaatatttg ttttttacca cttaaatatt gacccgttat tgagttctca ttttcaatta
    31321 tttcatctaa agtaccctgt gcaactattt caccaccatg tatacctgca cctggaccaa
    31381 tgtctataac ataatcagct tctctcattg tgtcttcatc atgctctaca acgattaagg
    31441 tatttcctaa atctgttaga tgtctaagag tagttataag cctatcatta tctctttggt
    31501 gaagccctat acttggctca tcaagtacat ataaaactcc aaccaatgca gaacctattt
    31561 gagtagctaa tcttatacgt tgcgcttctc ctcctgataa agttccagct tttcttgata
    31621 aatttaaata atcaagacct acatctctta agaacatagc tcttcctttt atctctttta
    31681 taatctcatg agctataaat ctctgttttt ctgtaagatt tatattttcc ataaagtcaa
    31741 tcaattcatt tacagataag tttgtaactt ccattatatt ttttccatca actaaaacag
    31801 ataaaacttc ctttttaagt ctcatccctt tacactttgg acatggtctc tctgccatgt
    31861 attcttcaat cttatctctc atgtaatctg aattagtttc tctatatctt ctctctaagt
    31921 ttactattac accttcaaag tatgatttat aatttctagt tcctccaaat tttgactcat
    31981 aagtaaactc tacaattata ttatctttac cataaagtaa ttcctccaca aaatcatctg
    32041 gtaaatcttt aaacggtgtt gtcaaagata cattaaaatg ctctgctaaa ctttttatca
    32101 ttttactgta ataagtgtca tcattaacac ctgttgaacc ccatgctgcc actgcacctt
    32161 gttttattga taaatctttg tttggtacta ctaattctgg gtcaacttct ttactttccc
    32221 caagaccatt acaaacatca caagcaccaa agggagcatt aaatgaaaac atccttggag
    32281 ataattcttc tatgccaatt ccatgttctg ggcatgcaaa tttagttgaa aaaagaattt
    32341 cttctccatc tattatttgg gctattacaa gaccatcaga tagcttaact gcagtttcaa
    32401 ttgaatctgc tagtcttgat tctattccat ctttaattac tattctgtcc actacaactt
    32461 ctatattatg tttttgattt ttatttaaga ttatatcatc actaacttca tagttctcac
    32521 cattaactct aactcttaca aatccttctt ttttgatatt atcaaagact tttttatgtg
    32581 tacctttttg tcctctaaca attggtgaaa gaatttgtaa tttcgttcta tctggaaaat
    32641 ctagcatttt atcaactatt tcttgaatag tcatctgact tattttttca ccacatgtag
    32701 ggcaatacac atctcctact cttgcaaata atagtctcaa ataatcatat atttctgtta
    32761 ctgttccaac cgtagaacgt gggttttttg aggtagtctt ctggtcaatt gagatagctg
    32821 gtgataaacc ctctatatat tctacatttg gcttttccat ctgacctaaa aattgtcttg
    32881 cataagcaga tagactttct acatatcttc tctgcccttc tgcatatatt gtatcaaatg
    32941 ccaaagaaga cttcccagac cctgacaaac ctgtaaaaac aataaattta tctcttggta
    33001 attctaaatc tatatttttt aaattatgtt cttttgctcc tcttattata attttatctt
    33061 ccataatgtt gtttttacct ttctattttt ccaatttttc ttctaattgt ttcactttat
    33121 ctctaagttc tgctgctctt tcaaattgta agttttgagc agcttccatc atctctgatt
    33181 ttaatttatc tatattagct cttatttcgt ctttatcatc tgtatcagat attccatata
    33241 caacttcttc ctctgcaacc ttagtagctt ctatactatc tcttatattt ttctgtatag
    33301 ttttaggaat tatattgtgc tcttcattat ataaattttg tatatctctt cttctctttg
    33361 tttcatcaat agcattttgc attgagtcag ttatcctgtc tgcatacata attactcttc
    33421 cattttcatt tctagctgca cgtccaatag tctgtattaa agctgtttcg gaacgtaaga
    33481 aaccttcttt gtctgcatct aaaattgcta taagtgaaac ttctggtata tccaatcctt
    33541 ctctaagtaa gtttattcct acaaggacat caaacttccc taatcttaaa tctcttatta
    33601 tttctgttct ttctaatgta actatatctg agtgtaaata tttaactttt atccctattt
    33661 ctttaagata gttagtcaag tcctcactca tttttttagt taaagtggtt ataagcactc
    33721 tttcattttt ttctatagtt ttagttattt ctcctaccaa atcatctatt tgattattta
    33781 taggtcttac ttctacaatt gggtctaaaa ggccagttgg tcttattatc tgttctgcta
    33841 ttgtctctga gtgttgaatt tcataaggtc ctggtgtagc acttacaaac aacatttgat
    33901 ttatatttcc ttcaaattct tgaaaattta atggtctatt gtcaagtgca gaaggtaatc
    33961 taaaaccatt ctctataagt gatgtttttc tagacctgtc tcctgcatac atacctctta
    34021 cttgtggtat agtgacatgt gcctcatcta caattatcaa ataatcatct gggaagaaat
    34081 ccattaaagt atatggtctt tctccttctg accttcctgt aatatgtcta gaatagttct
    34141 ctatcccctg acaaaatcct atttccttta acatttctat atcatactta gttctttgtt
    34201 caattctttg tgcttctaat agcctatcat tttccttgaa aaatttaatt tgttcttgaa
    34261 gctcgttttc tatttctaca attgcctttt caattctttc tggcgttgtt acatagtgag
    34321 atgctgggaa tataacaaca tgatttctag taccaacaat cttccctgtt acataatcaa
    34381 tttctgttat tctatctacc tcatcaccaa aaaattctat tcttattgct ttttcatcat
    34441 tactagctgg gaatatttct agtatatctc ctctaactct aaaggtacct cgtgtaaagt
    34501 ttatgtcatt tctttcgtat tgtatctcta ttaacctttt tattacatca tctctgtctc
    34561 tttgcatacc tggtcttata gataacataa gttctttata gtcttttggg tcaccaagtc
    34621 catatataca agatacagat gatattatta tagtatctct tctctctaaa atagatgctg
    34681 tagcagaatg tcgaagttta tctatttcat catttatgct tgcatctttt tctatataag
    34741 tgtcactgtg agctacatat gcttctggtt gataataatc atagtaactt acaaagtatt
    34801 caactgcatt gtctgggaaa aattctttaa actcactgta aagttgtgct gctagagttt
    34861 tattatgagc cattattagg gtaggtttct taacttgttg aatgatattt gccattgtga
    34921 atgtttttcc tgaacccgtt acacctaata atgtcgaaaa tttctcattt ctatttatag
    34981 aatccactat tgatttaata gcttctggtt ggtcacctgt aggtttgaag tctgatttta
    35041 ttttaaaatc catagtatca ctcctttttt cgaacattag ttcctgacat gtattattat
    35101 atcatacctt tataaaattg tttaagttat tttaaatatt ttacccattt aaattttact
    35161 taaatctaaa acataaaaaa agacttctat ctttgcaata gaagtctata attcatggaa
    35221 aataaatact ttattcaact tacataataa aatcaaatgc ttctttttca actccatcta
    35281 attttaaatc aaacattttt aatatagttg ctgcatgtgc tctcaggtct ccttcttcta
    35341 taaccacacc acctttaata tttggtccaa aagctataaa tggaggtcta ttacctttag
    35401 taggtaaatg accatgtgtc gcaacagcaa acttgtaatc ttttatatct gaatctttta
    35461 taatacttcc ttcgaaatca tttccaaatg aaattccttc aagaccttca atcaccattt
    35521 caaaatcacc ttcaagacca aggttagatg cttcctcttt ggtaaaaata ttctctatga
    35581 ataattcttc tttaaattct tcaagcagtt ctcttaattc tgttaaccta tctaagttat
    35641 ttactattat ttgagctgat attccagaag aatttacata ggcatcaaaa tccttaactt
    35701 ctccattttc aacttttata aatccatttg atttaagcaa gacatttgga tttataactt
    35761 tatcaactct aagatgacca tggtccccaa gtattataaa atttgtatct ttgtatgtac
    35821 ctgcatcctt tgtagcctga ataatatctc ctagccattt atcattcatt tttaatgctt
    35881 tatctacttc gtcattaaat aagccataat tatgtcgtgt atggtccaat gtagctaaat
    35941 gtattagcat taaatctggt ttatattcct ttattatatc tactgaacag tttactccaa
    36001 ataaatccat gttgggttct aatttccaat caatataatg acaatgtcta ttgtaaatac
    36061 cgtccattat aaccttactg gagcttcgtt caaaagtttc tcttgggtca tcttctctac
    36121 taggagccca tatttctgca atattataat ctatatttgg atttgctccc attacaggcc
    36181 ataatacact agaggtagtt agattatttt cttttgctac atctataata gtttttactt
    36241 ttatgtcctt tgaataccag taccagtctt tattttcttt atttgggtca aatttttcat
    36301 tatgatatat accatgtttt ttaggatata ctcctgtaac catactagta tgacacggat
    36361 atgtcagagt gggataaaca caatttatat tttttactat ggagcaattt tctagtatag
    36421 atttaaagtt tggtagattt tttacaaatt ccaagtcttt ttcaaataaa gaatctactg
    36481 ataatactat aacctttgtc atttacatta cctcttcatt tatatattat tgaatacttt
    36541 taatcaactt acaagtctat ataattattt atttgtttta tctattactg cttgcattgc
    36601 agaagttcct ttgcttacta catcttttgg atttgcattt aaatcagtaa gccccttttt
    36661 aatctcatcc attagcttat cagttgcttc cttgtatcca tttaacattg gaagtgcaac
    36721 agcatattgt aattggtcag ttgcaacctt atattgaggt ttttctttat ataatgtttg
    36781 taacttttct gagtttgttg catcttcagt tgttggtaag tatcctgttg atgaactaaa
    36841 ctgaacgatt ttatctgttt gtgtcatcca ttctataaat tctgcacttg cttctttttc
    36901 tttatctgtt ccaccttcaa gcataactag attagctcca cctgtaggag ttgcgtattt
    36961 ttgatttttt ggtaggaatg ctgttgatat ttcaaactta ccttctgttt gttgcataag
    37021 tcctgctaaa tctgctgttg atgtaaatat catacctact ttaccattta caaaatcttg
    37081 ttttgctacg tcccaggcat tataatcttc tccaggaggc attttcatta ttccagcttt
    37141 catcatatct tgccagaatt taactggtgc ttgtcctgcc tcattattaa atgctacttt
    37201 tttaccatta gttatttctc ctccactttg cattaccata gcttcataga accaaatatc
    37261 tattggtgtt tcaaaaccat aagtatcttc tgatttattt gtcattttac tagcgtaatc
    37321 cttaagctct tcccatgatt ttggacctga agggtcaaga cctgctttag ttgccatatc
    37381 tctatttatg tataatattg gtgtacttct attaaatggc ataccaacaa acttatcctc
    37441 ataatatgaa tttgttaaaa gaccagaaaa gaatctttct tgatatgttt tatcaaaata
    37501 tttactcata tctactaaag cacctgctga tgcgaatgta cctgtttgag ttatttctaa
    37561 taatgttaca tctggttggt ctttagatgt taaggctgcc tgtagttttg ttgcattttc
    37621 atagtaatct ttctgtttta acatatttac ttgtacatcc ttatgttcac tattaaattg
    37681 gtcaaccatt ttttgagcag tttctccatt agttccacct aaaggagccc agaaatctat
    37741 cttcgtaata ttgctatcag tcttttctcc tttagatgca tttgcatcat tagaatttga
    37801 acacccaact aaagaaccta ataataatgt tattgctaat cctgttgcta attttctttt
    37861 aagcttcatt ttcatcctcc aaatttagtc tttaattttt tattttattc ctgaatacat
    37921 aaaagcttct tttacttttt tattcgcaaa tatataaaca agaattattg gtaaaattag
    37981 aattatattt cctgccatta ttacattcca tgcagtaatt ccttctgaag atttaaggag
    38041 cgcaactcct attggtaaag ttcttattgc atctgtattt gtcattacta agggccagaa
    38101 ataattattc caatggtata taaaattaaa taatataaat gttattactg ctggttttgc
    38161 cataggaacc attattctaa acattatttt taagtcagaa gcattatcta gtttagctgc
    38221 ttcaatagtt tcatctgaaa tctgcataaa attctgtcta agcagaaata tcccaaatga
    38281 agatgtcata aatggaatta taagtggaat ataagtattt ataaatccaa acttactcat
    38341 catcatatag actggtagaa aagttacttg tggtggaatc ataagtccta caagcaaaag
    38401 cccaaataac attttttcac ctttaaattt aatcttagaa aatgcatatg ctgctggaac
    38461 cataactaaa aattgtaaaa ttaaaacact tcctgttaca aatacactgt ttagtaaata
    38521 tctaaaaaat ggacctgaat gccatgctgt actataattt tctaacacta tatcatttgg
    38581 tataatagta ggtggtaatt gcatcatttc ttgtggattt ttaaaggatg tcgatagcat
    38641 ccatataaaa gggaatataa atattgcaga taaaagtaat agaattaata tttccattga
    38701 ttttgatgct atttttttta tatcaacttt gtcttttttg ataggttgta taatatccat
    38761 tgtctcctcc ttttggataa atcttataat atctactttt tctttattga taatgaactt
    38821 tctttgataa gagtctaaag ttagcatatg tagcaatcat aactatagct aacaaaaaca
    38881 tactcgctgc actcgcttgc cctatgtcat agtatataaa tccagactca tatatataat
    38941 aagataacat gttagttgaa cccactggcc caccttgtgt cattatactt atctcatcaa
    39001 atacttgaaa agatgcagtt gttgttgtta tcataagaaa aaatattgtt ggtgataata
    39061 gcggcaatgt cactttaaag aaattcgtta ttttgtttgc attatctaat ttagatgctt
    39121 catatatata gtttggtatg gatttaagac ctgataatac tattaagata ttgtatccaa
    39181 gagtcttcca tattcctatt agaattagtg acattaaagc tgaactttca cttgaaagcc
    39241 actttgatgt tggtaaatgt aatgtcgata gtatccagtt taaaaagcca tattgtggct
    39301 ccataatcca aagccacaat acagctacag atactagtga aattacatgt ggtgtaaaga
    39361 ttatactttg cgttaaattg tgtattatag aatttttatt tagccaaaca gcaagtacta
    39421 tggctagtga tatagatata gtcaccataa aaaaagtata aattgcagtg ttcgttatag
    39481 tttgtataaa ctctggattc tttaaaagtt ctgtataatt gtttaagcct acattttcaa
    39541 aggttgaact aagaagacta ccagatttga agcttagaga tatcgtctta attattggat
    39601 agactataaa caataaagtt cctataatag caggtgttat atataaatat gctttattaa
    39661 attgcttttt cataaatatc tcctttaatt ttagaataca tacttttaca tacttttaca
    39721 tcttctattc tttctccatt tttatcaaaa taatataaat ctttttctaa tacacttatt
    39781 cttaaagttc ttccaatatc aaatatatca ttttccgttt tgactcttat ttctctatta
    39841 agaacagata aacaatatat agtttcattt cccaacactt ctttagtcac aacttttcca
    39901 aataggctaa atacatcatc attttttcca tctatgttta tactctctag tgttgctttt
    39961 tctggtctga atccaaattt aatatcattt tttatatcta gtatgttcat acaaggactc
    40021 cctataaatt gagctacaaa taaattctta ggattttgat atatcttctt tggagaacct
    40081 atttgcatta tctttccttt gttcattata actatcttat ctgccattga cattgcctca
    40141 acctggtcat gagttacata aacaaatgta gattttagtt tttgatgtaa ttgtattaat
    40201 tctgttctca tttgatttct taatttagca tctaaatttg acaatggttc atccattaaa
    40261 aatacctttg gatttttaac catagctcta gcaagtgcca ctctttgtct ttgacctcca
    40321 gataagttgc ctggtttcac atttaaatat tctctcaatc ctactacatc aataacttct
    40381 gttataagtt tttctctttt ttctttttct acttttttgt ttttaagacc aaattctatg
    40441 ttttctctaa cagtcatatg tggatataat gcataatttt gaaataccat agctatatcc
    40501 cttttactag catccgtttt tgtaacgtca ttttcttcaa tttttaactt gcctgaagtt
    40561 atatcttcaa gacctgcaat cattcttaat gtagtagatt taccacatcc acttggacct
    40621 accaatactg taaaagaacc atcttctata gtaagattta aattttctat aactgtattt
    40681 tttttattat aagattttgt tacattttct agtcttatat ctgccatttt tatcctccca
    40741 aaatatttaa tttaattcta cgatttactt atagattaat aatactctta atttgtaaag
    40801 gatatattaa gacaaagtga atatatagta aacttattaa aataaaggtt aaattataaa
    40861 cattttttgt taaatatact gaagttaaga tgatatacag cttatgttag ctataatttt
    40921 ctatattttt catattcatg gtttgctatc acaatgtgat tttaatttat gaagagtcat
    40981 ataaaactat ttatttttta tataatttgg tttgaaaata aaaaagacct ctatggggga
    41041 tagaggtctt taagtggggg agattgagta atttattttt gctatattaa gttttacaat
    41101 tatattatgt cccaaattta caaggtttaa acataaaaaa taaaactttt ttaattttta
    41161 taaatttacc
//
LOCUS       DAELSY010000017        40535 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.017, whole genome shotgun sequence.
ACCESSION   DAELSY010000017 DAELSY010000000
VERSION     DAELSY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40535
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.017"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..2276)
                     /gene="slpA"
                     /locus_tag="KPG09_000815"
     CDS             complement(<1..2276)
                     /gene="slpA"
                     /locus_tag="KPG09_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBG0965441.1"
                     /translation="MNKKNIAIAMSGLTVLASAAPVFAAEDMSKVETGDQGYTVVQSK
                     YKKAVEQLQKGLLDGSITEIKIFFEGTLASTIKVGAELSAEDASKLLFTQVDNKLDNL
                     GDGDYVDFLISSPAEGDKVTTSKLVALKNLTGGTSAIKVATSSIIGEVENAGTPGAKN
                     TAPSSAAVMSMSDVFDTAFTDSTETAVKLTIKDAMKTKKFGLVDGTTYSTGLQFADGK
                     TEKIVKLGDSDTINLAKELIITPASANDQAATIEFAKPTTQSGSPVITKLRILNAKEE
                     TIDIDASSSKTAQDLAKKYVFNKTDLNTLYRVLNGDEADTNRLVEEVSGKYQVVLYPE
                     GKRVTTKSAAKASIADENSPVKLTLKSDKKKDLKDYVDDLRTYNNGYSNAIEVAGEDR
                     IETAIALSQKYYNSDDENAIFRDSVDNVVLVGGNAIVDGLVASPLASEKKAPLLLTSK
                     DKLDSSVKAEIKRVMNIKSTTGINTSKKVYLAGGVNSISKEVENELKDMGLKVTRLAG
                     DDRYETSLKIADEVGLDNDKAFVVGGTGLADAMSIAPVASQLRNANGKMDLADGDATP
                     IVVVDGKAKTINDDVKDFLDDSQVDIIGGENSVSKDVENAIDDATGKSPDRYSGDDRQ
                     ATNAKVIKESSYYQDNLNNDKKVVNFFVAKDGSTKEDQLVDALAAAPVAANFGVTLNS
                     DGKPVDKDGKVLTGSDNDKNKLVSPAPIVLATDSLSSDQSVSISKVLDKDNGENLVQV
                     GKGIATSVINKLKDLLSM"
     gene            complement(2502..4091)
                     /locus_tag="KPG09_000816"
     CDS             complement(2502..4091)
                     /locus_tag="KPG09_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp12"
                     /protein_id="HBG0965442.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGTVKAPILLSQRNSIPSDTEKMLKDVKKV
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGNDRIETSYLIAKEIASIKPIDKVFITNGY
                     TGEADAMSASSVASRDGSPIILTNGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNIVAK
                     DSNGNDLTPELIGNIDTDKVGKYKVTIKATDIGGKTTSISVEVNVLEYKTNDMNSSEF
                     KRMVSSEMYSLVNSYRKEKGKEPLQVSKNLEGLSNSWSKYMADKGQFSHVINGKKSVE
                     VFTGYGLRSEENIAFVPLVTKSTYTTKDAREVANVIFTVWKKSDKYNENMLNSDFVYT
                     GFGLYILPNGEVYATQEFLNK"
     gene            complement(4310..5911)
                     /locus_tag="KPG09_000817"
     CDS             complement(4310..5911)
                     /locus_tag="KPG09_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBG0965443.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADRQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASKSNGQPPTITVGSTEIYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSYDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKNLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            complement(6278..8158)
                     /locus_tag="KPG09_000818"
     CDS             complement(6278..8158)
                     /locus_tag="KPG09_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBG0965444.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSVTCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTSDKPALK
                     IVTYNNKTVDYENINSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKITKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGSSSNSTSNVTVKKLKGADRFETA
                     TKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAVQFIKSNKVSNIYFIGGI
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVVDKIKSLI
                     EK"
     gene            complement(8368..14331)
                     /locus_tag="KPG09_000819"
     CDS             complement(8368..14331)
                     /locus_tag="KPG09_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HBG0965445.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGN
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNIGTDNIEYTIK
                     KLSASRSEIKNKDENVSYYNIVFNNLPVGKYNIEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDEDGNVQQ
                     IPYENKEYMKNLKDKEQQEEVNNEQEEVNQEEVNNKDENKNQTDNNSNNKDENNDKQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEDTPKEGDNISKDDNSTTSTYSRAALE
                     TYTDVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSLKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESIDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEIRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIDRELKLAQDLYNDRATLTDVTTVNQ
                     EINKSGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLKTGKNVIQLSKLHSLDIECGGSLYIRYPEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNIGNSY
                     EYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKAKFTDSISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDVSTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPEAETRKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDDKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADQTRTNGIGKLAEDYVYQSDDPSTSEDETGKIPAGS
                     IIITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNAIDVSNKKVITVSNKVKGNIVRDK"
     regulatory      complement(14455..14540)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(14712..16115)
                     /locus_tag="KPG09_000820"
     CDS             complement(14712..16115)
                     /locus_tag="KPG09_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBG0965446.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILIDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALVASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     DNGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKISDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     gene            complement(16577..18472)
                     /locus_tag="KPG09_000821"
     CDS             complement(16577..18472)
                     /locus_tag="KPG09_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBG0965447.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKSETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            complement(19098..19241)
                     /gene="scfA"
                     /locus_tag="KPG09_000822"
     CDS             complement(19098..19241)
                     /gene="scfA"
                     /locus_tag="KPG09_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBG0965448.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(19307..19669)
                     /locus_tag="KPG09_000823"
     CDS             complement(19307..19669)
                     /locus_tag="KPG09_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBG0965449.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(19727..20020)
                     /gene="yajC"
                     /locus_tag="KPG09_000824"
     CDS             complement(19727..20020)
                     /gene="yajC"
                     /locus_tag="KPG09_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBG0965450.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(20154..21275)
                     /gene="tgt"
                     /locus_tag="KPG09_000825"
     CDS             complement(20154..21275)
                     /gene="tgt"
                     /locus_tag="KPG09_000825"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBG0965451.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITSIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPQDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(21354..21839)
                     /locus_tag="KPG09_000826"
     CDS             complement(21354..21839)
                     /locus_tag="KPG09_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965452.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(21851..22876)
                     /gene="queA"
                     /locus_tag="KPG09_000827"
     CDS             complement(21851..22876)
                     /gene="queA"
                     /locus_tag="KPG09_000827"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBG0965453.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFLYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGNNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(23194..24213)
                     /gene="ruvB"
                     /locus_tag="KPG09_000828"
     CDS             complement(23194..24213)
                     /gene="ruvB"
                     /locus_tag="KPG09_000828"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBG0965454.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(24320..24922)
                     /gene="ruvA"
                     /locus_tag="KPG09_000829"
     CDS             complement(24320..24922)
                     /gene="ruvA"
                     /locus_tag="KPG09_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBG0965455.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASSAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(24915..25424)
                     /gene="ruvC"
                     /locus_tag="KPG09_000830"
     CDS             complement(24915..25424)
                     /gene="ruvC"
                     /locus_tag="KPG09_000830"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBG0965456.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(25736..26365)
                     /locus_tag="KPG09_000831"
     CDS             complement(25736..26365)
                     /locus_tag="KPG09_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965457.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKNNGVQTSK
                     VVSASANMDEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMNATIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            26568..26891
                     /locus_tag="KPG09_000832"
     CDS             26568..26891
                     /locus_tag="KPG09_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG0965458.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            27159..28013
                     /locus_tag="KPG09_000833"
     CDS             27159..28013
                     /locus_tag="KPG09_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965459.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(28199..29635)
                     /locus_tag="KPG09_000834"
     CDS             complement(28199..29635)
                     /locus_tag="KPG09_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBG0965460.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNELLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPVETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTHGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKEMLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(29706..31088)
                     /locus_tag="KPG09_000835"
     CDS             complement(29706..31088)
                     /locus_tag="KPG09_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG0965461.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAVLIGGWIYGKRFEKYPAYAIDENDREKN
                     LEKGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKRYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(31224..32129)
                     /gene="garR"
                     /locus_tag="KPG09_000836"
     CDS             complement(31224..32129)
                     /gene="garR"
                     /locus_tag="KPG09_000836"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBG0965462.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            32525..33190
                     /locus_tag="KPG09_000837"
     CDS             32525..33190
                     /locus_tag="KPG09_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0965463.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDVYKTTLIHMETPKYINLEDFC
                     NNK"
     gene            33298..33846
                     /locus_tag="KPG09_000838"
     CDS             33298..33846
                     /locus_tag="KPG09_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965464.1"
                     /translation="MIFLNENVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTIEILRKVLFERSEERAIAMRERALENGDELIADNIISTTDVPFLGWVPEFKE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            34108..34416
                     /locus_tag="KPG09_000839"
     CDS             34108..34416
                     /locus_tag="KPG09_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965465.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(34578..36368)
                     /locus_tag="KPG09_000840"
     CDS             complement(34578..36368)
                     /locus_tag="KPG09_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0965466.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(36368..38095)
                     /locus_tag="KPG09_000841"
     CDS             complement(36368..38095)
                     /locus_tag="KPG09_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0965467.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSARVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(38340..38904)
                     /locus_tag="KPG09_000842"
                     /pseudo
     CDS             complement(38340..38904)
                     /locus_tag="KPG09_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
     gene            39193..40305
                     /locus_tag="KPG09_000843"
     CDS             39193..40305
                     /locus_tag="KPG09_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG0965468.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     AEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
BASE COUNT        12539 a         7205 c         4648 g        16143 t
ORIGIN      
        1 tacatactta ataaatcttt taatttattt ataactgaag tagctatacc tttaccaact
       61 tgaactaagt tttctccatt atctttatca agaactttac ttatagatac actttgatct
      121 gaagataaag aatcagtagc taatactata ggtgctggag atactaattt atttttatca
      181 ttatcagaac cagttaatac tttaccatct ttatctactg gcttaccatc agaattaaga
      241 gttacaccaa agtttgctgc aactggagct gctgctaaag catcaactaa ttgatcttct
      301 ttagtagaac catctttagc tacaaagaaa ttaactactt ttttatcatt atttaagtta
      361 tcttgataat aagaagattc ttttataact tttgcattag ttgcttgtct atcatctcca
      421 ctatatctat ctggagattt acctgtagca tcatctattg cattttcaac atctttagat
      481 acactgtttt ctccacctat tatatcaact tgtgaatcat ctaagaaatc ttttacatca
      541 tcatttatag ttttagcttt tccatctaca actactattg gtgtagcatc accatcagct
      601 aaatccattt taccattagc atttcttaat tgagatgcaa ctggagctat actcatggca
      661 tctgctaatc ctgttcctcc aactacaaat gctttatcat tatcaagacc tacttcatca
      721 gctattttta gagaagtttc atatctatca tctcctgcta atcttgtaac tttaagtccc
      781 atatctttta attcattttc tacttcttta gatatagaat taactccacc agctaaataa
      841 actttctttg aagtatttat acctgttgta ctctttatat tcataactct ctttatttca
      901 gcttttacgc ttgaatctaa tttatctttt gaagttaata ataaaggagc tttcttttca
      961 gaagctaaag gagaagctac aagtccatca actattgcat ttcctccaac caatactaca
     1021 ttatcaactg aatctctaaa tatagcattt tcatcatcag agttataata tttttgactt
     1081 aatgctattg cagtttctat tctatcttct cctgctactt ctatagcatt tgaatatcca
     1141 ttattatatg ttcttaaatc atccacataa tcttttaagt ctttcttctt atctgactta
     1201 agagttaatt taactggtga attttcatca gcaattgaag cctttgcagc actcttagtt
     1261 gtaactcttt ttccttctgg ataaagaacc acttgatatt ttccactaac ttcttctact
     1321 aatctattag tatctgcttc atccccattt aatactctgt aaagagtatt taaatctgtt
     1381 ttattaaata catatttttt agctaaatct tgtgctgttt tactagaact agcatcaata
     1441 tctattgtct cttcttttgc attcaatatt ctaagtttag ttattactgg gcttccagat
     1501 tgtgttgttg gtttagcaaa ctcaatagtc gcagcttgat catttgcact tgcaggtgtt
     1561 attattaatt ctttggctaa atttatagta tcactatctc ctaatttaac aattttttct
     1621 gtttttccat ctgcaaattg aagacctgtt gaataagtag ttccatcaac taaaccaaac
     1681 tttttagttt tcatagcatc ttttatagta agtttcacag cagtttcagt tgaatctgta
     1741 aaagctgtat caaatacatc tgacatagac ataactgcag cactacttgg agctgtattt
     1801 tttgctcccg gagtaccagc attttcgact tcaccaataa tactacttgt agctactttt
     1861 attgcactag ttccacctgt taaatttttt aatgcaacaa gtttacttgt agttacttta
     1921 tctccctctg ctggagagct tattaagaaa tctacataat ccccatcacc taaattgtct
     1981 aatttattat ctacttgtgt aaacaataat ttacttgcat cttctgcact aagctcagct
     2041 cctactttta tagtagatgc taaagttccc tcaaagaaaa ttttaatctc tgttatactt
     2101 ccatctaata accctttttg taattgttca actgctttct tatacttgct ctgtactact
     2161 gtatatcctt gatcaccagt ctcaactttc gacatatctt ctgctgcaaa cacaggtgct
     2221 gcagaagcta atactgttaa tcctgacata gctattgcta tattcttctt attcatttct
     2281 taaattcctc ccaacattat attaaaatta catctcttca ttatgtacat agaataatca
     2341 tccatatatt cctaatattt ctcacatctc tataataatc catttttatt ctttttgcaa
     2401 gtcatttttt tgaaattatt atttttccca agcatatgag tttttatacc cttaaactcc
     2461 aatatttaat aacttaaaaa atttgatttt acttatttaa attacttatt taaaaattct
     2521 tgtgtagcat acacttctcc attaggcaat atatataatc caaacccagt atatacaaaa
     2581 tcactattta acatattttc attatattta tctgactttt tccaaactgt aaatattaca
     2641 tttgctactt ctctagcatc ctttgttgta taagtacttt ttgtaacaag tggtacaaaa
     2701 gctatatttt cttcacttct caagccatat ccagtaaata cttcaacaga tttcttacca
     2761 tttattacat gtgaaaactg acctttatct gccatgtact tagaccagga attagaaagt
     2821 ccttctaagt ttttagaaac ttgtaatggc tctttacctt tttcttttct atatgaattt
     2881 accaaactat acatttcact agaaaccatt cttttaaact cactactatt catatcatta
     2941 gtcttatatt caagtacatt tacttctaca cttattgatg tagtctttcc accaatatca
     3001 gtagctttta tagtaacttt gtatttaccc accttatcag tatcaatatt tcctataagc
     3061 tctggtgtca aatcgttacc attgctatcc tttgctacga tattcagttt acttatatca
     3121 aacttatcac cttgataaat atttaaattt ccaacagtta ttgttggtgc acttgcatcc
     3181 agtgatgatg cacttaaaca ttgttctagt gtcttttcat ctattcctcc tagtgctgta
     3241 atcttatcag cacttctaag tacagactta tcactatttc cgtcaactaa tacaataggt
     3301 gcattttttg ctatagaaga accagcaact gcatctacta attgataacc tttagaaaga
     3361 taaaattctt ttgccgaact ataaaaatgt tttataactt ttttattagt ctcaaatcta
     3421 tcttctccag ctaatctaac tgcattagtt tttttaacta aatcattact tattatctcc
     3481 tctgaaccaa gagcataaca ttgcacacct tcttttttct caaatggaac atttttacca
     3541 ttagttaaaa ttataggtga tccatctctt gatgctactg atgacgcact catagcatct
     3601 gcttcacctg tataaccatt agttataaaa accttatcta taggttttat tgaagctatt
     3661 tcttttgcta taagataact tgtttcaatt ctatcattac cacctattct tttaacttct
     3721 attccttttt gctttaattc attttcaact gattttccta ttgaatcttc agttcctatt
     3781 atataaacct tctttacatc ttttaacatt ttttctgtgt ctgatggtat actgtttctt
     3841 tgtgataata atataggtgc tttaactgtt cctgctaatc cacttgcact taatccatca
     3901 gctagtgtat tatctgtatt tactaatact acattttcat atgtttgttt ctgtgcaatt
     3961 ttggcagctg tctcatatct atctttccct tgtatctttt ctatgctcga aagtgcattg
     4021 atatttggtg ctcctgccat tattaatgat attgtcaatc ctattgataa tactctctta
     4081 tttaccttca tcgaactcct cctactaatt tttttatata attcagctaa tcataaatta
     4141 catcctcagt agtgattccc tcaaaatcta taaactacta aaacaaaaaa cacctaaaat
     4201 accatagcct gtaaagcaat aacaaaacag attacaaaaa gtactttaga tgtgattttc
     4261 attttataat atcagtaaat taacaaaaaa acaagattta tggaattttt tacaaactac
     4321 cttcattgcc atttaaaaac tcctgagtag catgcacttg accatcagat aatatataaa
     4381 gcccaaatcc agtactatag aactcatcac taagcatgtt tgcattgtac tctggagact
     4441 tcttccacac ttcaaatata gcctttgcca agttttttgc atcttgtgtc gtttgaacgt
     4501 tcttagaatc tatataaata tatgctatat tttcctcact tctcattcca aactcagaaa
     4561 atacttgtgg agcatttttt ccatctatat aatgtgcaaa tactttttta tccatcatat
     4621 acttagacca tgcatttgcc ataccttcta gcctactaga tactaccaaa ggctctttgc
     4681 ctttttcttt tctataggaa ttgattaagt tatacatttc agtagatacc atttttttaa
     4741 attctgggct gttgtaatca taggatttat catccaacac ttttatttct actctttttc
     4801 ctgtactttt tccccattcg tctgtagctt ttaatgtcaa tatgtatgta cctactctat
     4861 ttgtatctat aaacccatca acttctattg gtaatacctt cccagtgtta tctttagcca
     4921 ctatgtttaa cttgccagtg tcaaactttt cacccttata tatctctgta cttccaactg
     4981 taattgttgg tggttgtcca tttgactttg aagcatttat acactgttgt attaccttct
     5041 catttatttc acctacagaa gttatattct tagctccttc taatacagtt ttatcgcttc
     5101 catcatttac tagtaccatt ggagagtttt tagtaagtgg tgcagctgct attgcatcta
     5161 ctaattggta tccatctgat acatagaatc catctgcact attaaaaaag tagtctatta
     5221 catttttgtt agtttcaaat cggtcagttc cttctattct tactgagttt gtatttttaa
     5281 ctagagggtt actcattatc tcctctgaac ctatacaata tgattgaata ttttttacat
     5341 caaaaggcac actctttcca tctgtaagta tgattggagc tccatctcta gtagctactg
     5401 atgatacact cattgcgtct gcttctccac tatatgcgtt agttaatagt actttatcca
     5461 ctttttttat agttgctatt tctttagcta ttaaatagct tgtttttagt ctatcttctc
     5521 caccaattct tttgacttct atatttttag aatctagttc tttttctact gatttactta
     5581 tagtatcttc tgtacctatt atatatgctt ttttgatatt ttttagacat ctatttgtgt
     5641 ctgctggtat cttattttgt tgtgtaaata gtattggagc ttttgtagct cccgataatc
     5701 cacttgcact taatccatct gcaagtgatt tttctgtatt tactaaaatt actgtttcat
     5761 aagtctgtct atcagctatc tttgctgctg tttcatatct atctttaccg tatatttttt
     5821 ctactactga tgatgcattt acacttttga agtttactaa gattaatgat actgctagac
     5881 ctaatgataa tatttttttg ttcattttca tatccccctg atgattttta tattttatat
     5941 ttatattgtg attattttct atttattttc tctttatttt ccatatatta tagagtatat
     6001 atgatatgaa tatatctcgt ctatatatta gtgttttatt ttattaaatt taaaaaatta
     6061 tttttaactt ctttttaaac atattcaaaa ttgaatttat atttatatat ataaaagata
     6121 tctgctttat ttaaaattaa acttaatata gctcaaatta ttatacattt taacctcaat
     6181 attattaaat tgttaagata atgaaaataa aataagaatg catctaaata tataacttta
     6241 tatctaaata cattcttatt ttcgatttac ttaatattta tttttcaatt aaactcttaa
     6301 tcttatctac tacagatgag gctattcctc cacctacttc aaacaacttt gatgaatttt
     6361 taccttttaa tgatgattct tgtgatttat ctaacgtatt tgtagcaagt ataactggtg
     6421 aattgttttt agctgctaat ggtccaactg ctaaagcatc tataagttta tcttgatttg
     6481 caactacaac tccatctagt ttagattgag agtagaattt atctattact ttggcattcg
     6541 tctcttgtct agtttgacct gccaccctat tgtttgatat atctttgttt gctatttttc
     6601 caacttgttc aatagccttg ttagatattt tttctattcc accaataaag tatatatttg
     6661 acaccttatt tgatttaata aattgtacag cttcattatc tacaccatct ttttgtgtaa
     6721 gtactattgg agttttttct ttaccggcca gtgatgctat tgataatgaa tctgcttctc
     6781 catttcctgc ccctatatac aatttgctta cattattcgt cttatctatt tcttttgcta
     6841 tattaataga agtttgatat cttgtatctc cacctattct ctgtactgat atttttgaat
     6901 ttacagcttt gatagcttca acaactgaat ttggcattga gttgtttcca cctatcacaa
     6961 ttacttttga tggatttagc cttttcaatt cttctacagt tttttgagga agcttttcat
     7021 ttgatgataa aagaatcggt gagtttttta cactagcaag tggtgtagct gtaagcccat
     7081 ccaccatgct tttatcttct ccatttacta ttacaactgt atctgatttt gtccaaccac
     7141 tttgagatat tttggtagct gtctcaaatc tatcagcacc ttttaatttt ttgactgtga
     7201 cattagatgt agaattactt gaacttcctc ctccagaact tccaccacta gacgagccgc
     7261 ctgaattact tccaccacca ttatttccac cattgccatt atctggtggt aattcatccg
     7321 gttttgataa ttcattttca actatgtatt ctaaactttt tggaagtgta acttcaacat
     7381 atttatttat tgtagctttt cttcccttag ttattttttg tgctaatcca agttttaata
     7441 ttacatttga accactttct tgtgtttctt tactaacaat agaatagttt atcctaatat
     7501 tgtttaataa atattcaagt gcttcacttt tctctacctt atacttctca gggtcatttt
     7561 tactagaacc agttccacca attggtttta cttcactctc attaataagg gtaaatatta
     7621 atccttcatc agagtttata ttttcataat ccactgtttt gttgttatat gttacaattt
     7681 ttaaagctgg tttatcgctt gttccttttc ctaaaacttt atagtcagca tttgagttac
     7741 tgccagattt ttttacagat attcttccta tattcaatct atttccgtct agtaaatctt
     7801 catttgatgt aacatatata tctaatgtag ttgtattact tgatttgttg tattctactc
     7861 tacaagtagc atttgcattt ccacttttta tctgagattt tatattatca gaccaagtaa
     7921 tcttttcttt ctgctcatta tctactactt caacctcacc ttgtactttt atactggctt
     7981 gaaatgaata tactatgttt tctactgagt ccacagatat atctatagta tttactttct
     8041 catacgtttt tgttccttgt aaaactggag ctgcatatgc acatgtaaca ctaccagtta
     8101 cagctactgc catagttgat actgctatag ttcttataaa agattttctt ttactcatta
     8161 acacccgtcc ttatctatca ttaactaagt ggataaattt acttatccac ctagctttta
     8221 tatttaattt ataaattacc ctaaacttat agttacattt atatactttg cttttaatat
     8281 ctactatttt aaaactattc tagtacctaa tcatattaga tatacaatgt tagtattagt
     8341 atttttacgt ttttagagtt ccacctacta tttatctcta acaatattac cctttacttt
     8401 attgcttaca gttataacct tcttattact gacatcaata gcatttcttg tagcttttct
     8461 agctttacta ttactatcag taagacttat tgtctttaac tcatcataat tcttagctcc
     8521 tacaggtaca tacattgtat cacttacaaa tctttgacct actggcgtat ctccttgaag
     8581 gtcattgtat ctatatagtt cagccttaac cttatcactt aaagaatcaa agttttcttt
     8641 tggcatatag taaacccatg tacctttact gatttcacct aattcacctt tttctggaac
     8701 ttctgcaaat aaaagtgctt ctccagatat agtcttattg tttttatcta taagtagtgg
     8761 taaattaaat gctggatttc cactgtattc acctgtaata attattgaac ctgctggaat
     8821 cttacctgtt tcatcttcac ttgtgctagg gtcatcactt tgatatacat aatcttctgc
     8881 taactttcca attccattag tacgagtttg gtctgcatta ttcattgtgc caatttctat
     8941 attatcacct ggaggtccta ttatatctaa ttctgcaact tctatattag acttattgtt
     9001 tgagattagt tttacatatc tagcttggta tgtccaaagt ttatcatctt tattaaatgt
     9061 aactctagct tcattatctt ggtcttttcc tccaccaact aaatcttgtc catattcaaa
     9121 agttccttga cttacatctt cccagttctt tttatcatta cttacttgta tcttgtaatt
     9181 agttaaagga ttgtatgttg tgctagtatt ctttctaaag ttaaataatc cttttaatga
     9241 gaacttccta gtttctgcct ctggttttgt atattttagt cctactatag gtaatgtttc
     9301 tttcatgtct actattatat atggattttc ttctacattt atatcagggt ctttttgagg
     9361 gtctttgttc cactctgctt tacttattat ctttccttta tatgtagtac ttacatcatt
     9421 atcttttata ttatcaattt caggattttg taatggtcca tgtgagtcat tttcagttct
     9481 aacatcaccg tcagaaacag tatttgttat gaatgaccat gctgatttat ttattttccc
     9541 ttcatctgat aattttacag tgccaacttc aaattcttct gttggattta ggtgataatc
     9601 atatgcaact accttatatg taaatgctct gttattaaga ggagatatac tatcagtaaa
     9661 tttggcctta ctatttcctt ttgcatccac aaatccaact gttctgtatt ttacctgtgt
     9721 tccattttca ccatctaaat aatttccatc acttcttatt atttcgtatc ctagtatctt
     9781 atctttatcc ttatctacat ctaggtctat agatatttta ttagatttaa ctagagaacc
     9841 actttgtatt ccatcagaaa aactagcact tactttagta tcttttgcca tactagctat
     9901 attttcattt aaaatctttc ttctagcttc gtcatttaca tattgtatct ttcttgattc
     9961 tttttcatag ttcttactat ccattatact gtttaattct ggtgtaattt ctactcccca
    10021 tgccttaaag taatctctta aatctttttc aacaacactt gatgccattg ctactagcat
    10081 ttggtctttt ggtaaactat taattgggtc actacttgaa agtgctctgt attttcttga
    10141 aatctttgca tagaatgtat cattacttaa atcagcatct gagttattct ttaacattga
    10201 acccgtaaaa tcatcatcat aagctagatg taattgccat aacattccaa gcctttcaaa
    10261 tacattgctt ggtataccta agctatttga agttacatgc tcatatatct tatccattgt
    10321 tgaaccttca agtcttgaat gtgtcttatc atcaaaggtc tgagcaagta aagcaagtac
    10381 attgttacta gtttcgctat atgtcatctt tccaatatct gtaacatgtc ctatttcatg
    10441 acctattccc catccaaata agtttcctcc tgtagctttt ccactatcat ctatttggaa
    10501 aggtactcca ttcataagcc ctggaactga accatattct actcctacat gtaatccaga
    10561 agcatacata aatgctccaa taaacattct ttggtatttt atgttcatac gagattttgg
    10621 agccatatca tttttagtta agttaaattc attatcatct acagttccat taccatctaa
    10681 atcttgtgtt ttaaatactc ctctttctga gtaaactaaa tccattattt gttcccatgc
    10741 aagcatacta ttatataatc tatctacttg tgtatttaaa tcagcatttc cttttgatat
    10801 tccttgatat acagctgttg caggaacatt taatgttact ttatcacttt caatatctgt
    10861 agtgtttaac acaccagttt tttctgaata ttcatatgaa ttaccaatat ttgttcctgg
    10921 ataatatggt tctcctttta cagcatcttt atatttagct aattcttcta tatatgcttt
    10981 tatctttttc ttagattcag aaactcccgc aggactctta tcatttattg ttccatttag
    11041 attaagatgt ggtatcttag tagctcctgc tacacgtact tttatatctc ctccacttgg
    11101 agtatcttca gggtatctta tgtataaact tcctccacat tctatatcta aactatgaag
    11161 tttgcttaat tgaattacat tttttccagt tttaagattt atttctccac tcttatatgc
    11221 accagattct ccatagtact gtgtatatat caacttagga gtttttccac ttttacttcc
    11281 aacatatact gttatttcat ctcctgctct agcagctact cctaatgcct gccagtcatt
    11341 ttgcatgcct aagttattgg aatttacagt atactgtccg tttttatctt tatattttcc
    11401 tgatttattt atttcttgat ttacagtagt tacatcagta agtgtggctc tatcattata
    11461 taagtcttga gctaatttta attctctatc aataactgtc ttatatggat gatattctcc
    11521 actagcttta tctggagtat ttaatctatc ttttaattca gttatttttt cttgagttac
    11581 atcttctttt aattcaactt gtaggtcatc cataaataga tttcttacat catcttctat
    11641 actgtcatac tcataaaact taagttctga tatattagac ttatatgaat aagatgaaca
    11701 aacacttact tgtatcttag tagcagttat tggttctttt aatctaacca ttacatactt
    11761 attattattt acagggtctg tctttgtttc aaaaggaaca tcccccagat tgtgtaattg
    11821 accattttca tcccaatatc gtatctcaaa gtcatttatt gtcttatcag aaacatcaaa
    11881 acgaggtgta gccataaaag tatccatagt ataagattta tcaaaagtta ttattggtcc
    11941 tctattcgtt ccctcagctg cattatatgt tgggaatgtc caatgtgttg tgaagtctcc
    12001 atctactaca ttgaatttat caaatggatt tttatcacta tattcacttt tgtcatatcc
    12061 tgctggataa acaacatcta caatgtgatt tgttggtatt ccaaatccac cattctcctt
    12121 aggagtatta attaatctat agttaggtgt tgttggtgca tctatgcttt cagttgtacc
    12181 tatatatgtt ttagacattc cactagttcc caagtggtta gtagctgtca ttctaacttc
    12241 atatgttgtg ttatccttaa gaccttctat ttttaaactt gtcccacgaa taagcttatc
    12301 attgtatttt atccaagacc cattagagcc tttttcttta tagtacaagt caaagtcttt
    12361 agcttgttta tgatttttcc agcttacatc aagagattta aatttacctt taacatttat
    12421 accttctggt ggttctggtt ttgtgtctgg aacaacttga aattctaata tgctatctgg
    12481 gctaaattgt ataggtttat agtttgagtc tacattttct ggtactccat ctaagttttc
    12541 tggcgttgca tctttataac cactagacca atctccattt aaagattgta tgctaaccct
    12601 atatagtcca tatgcttcta tatctctaac ttctattgta tttttactag tttgtaattt
    12661 tttagtagat aatacttttc ccttactatc aattttatct agtataagtt cgtatgctga
    12721 tacatttact tcattattcc aataaaaaga tactttttca tttccaacag cagtgctagt
    12781 cgatatttta tttattttag gtatgttcat ctctggcttt ggtatttctt tatatacatt
    12841 gtttaaaaat tcaacttttg atatttctgc aagatttcta tcgtttgctg ttgtagctgt
    12901 tatttttata gttatctttt ttactgctac ttgtttacca agatttatta ctatatcgct
    12961 attttgaaca tctgtatatg tttctaatgc tgccctacta tatgtagatg ttgtagagtt
    13021 atcatcttta ctgatattat ccccttcttt tggagtatct tcatctgaat taggtttatc
    13081 cattgatgaa ttttctttat cattattttt atcttgattg gtattagagt ttacttcttg
    13141 tttatcgcta ttttcctgtt gtttatcatt attttcatct ttattatttg aattattatc
    13201 agtctgattt ttattttcat ctttattatt tacttcttct tgatttactt cttcttgttc
    13261 gttatttact tcctcttgtt gctccttatc ttttaagttt ttcatatatt ctttattttc
    13321 ataaggaatc tgttgaacat taccatcttc gtcagtaacc tcaactattc cttttgctgg
    13381 agcagtacct tctggagctt ttatattgat gacttccata ttaagtgctt ctgataaagc
    13441 tatatccatt actgctggtt tttcctcaga tatttcatca ttttttgaag atattgtaac
    13501 tccattattt tctccactaa ataaattttc tacattacca ttaatatcta catcactatt
    13561 tccttttaat tcaaccttac ttgtatctac tataggattg gtagatacta tactttctag
    13621 gctttttgca tcttcacttt gtcctaaatt tttctgtaca taatctaagt caacaatatc
    13681 tacctttcca tctctattta aatcaaattc tcttttattt gaatctatat tctctaaaac
    13741 cttattataa tcactcttat ctactttacc gtctccattt acatcaccca ttaagaataa
    13801 ttcataattt aaaggtttat ttttattatc tacacttgct ctattgctta ataagactct
    13861 ttgtgaataa cttgatatat caatgtcttt aatttctttg ttcttaaatc cagctccaga
    13921 gacttctata ttgtattttc caacaggtaa attattaaat acaatattat aatatgatac
    13981 attctcatct ttatttttta tttcacttct acttgcagat aattttttta tagtatattc
    14041 tatattatct gtcccaatat ttactgttcc tgaatctttt tctccaagct ttattattcc
    14101 actattgtta ctatcatctt ttaatcgtaa ctgcatatta gtcatacttt ctgactcgct
    14161 attttttatt ggcagtgaaa aatttaaatc tacttcaaga ttccccctta acttttctgt
    14221 aactgatttt tcaactgccc ttagcttccc ctctaatata ggttctgcaa aaacatttag
    14281 tggtgcacta cacatagata ctgctagtac acaagatatt gcctttttca tgaaacaaca
    14341 atcctccctt aataaatctc taatttttca tgaatttaaa tactataagt ttcagacaat
    14401 aaaaaaagac actcaaagag aatcttaatt ataaaataaa aagactttac tctttttagc
    14461 aactggctga tatggcttta aaagctttaa tccctatagt tttgcgtcat tagatttccc
    14521 taattttgcc atttaatttt cactactttc ttacccattt tatactatat ttaaactttt
    14581 taatacaatt tttttacatt aaattataaa aaaataacat ttatattaat aaattaatat
    14641 aaatgttatt ttccattata atttaattat ttatatagct tccttacaag cataattttg
    14701 tgtatatttt cttattcttt atatcttcca taaaattcat gtgtagcata tagattctta
    14761 tctcctgtta tagctatacc aaatcccatt atttcatacc ctttatgcaa catattagtt
    14821 ccttcttctg gatttgtctt ccactcatta aaaataaagt ttcctatatc atctgcatct
    14881 ctatttgtat atacaggttt atctgaaatt ttatatttaa ttatattttc accttgaact
    14941 gctatatatc caggtttaat ctcactccaa tctaagtatt tcaaaaaagt agaatatgaa
    15001 ttttttccat ttatcgtatg agataaagtc ttcttatttg ccattaaatt agaccaatca
    15061 tttgctagac cctctaatct gcttgctaca gataacgctc ttactccatt atcttttcta
    15121 tactgctttg taagagcata tactttaccc tttattctct ctttaaatgc tctatcattt
    15181 ggattgtaat ataacccttg ttgatttggt gaagcttgat ttatacattg cttgataaca
    15241 ttctgattta tgcctcctag tacagtcaat ttgtctgcac cacttaaaat actcttgtta
    15301 ctgccatttt caactaaaac tacaggagca ttttttgcta ttgtagaggc gaccaaagcg
    15361 tctgttaatt gcaagccttt acttagataa aattctttac tatttttata aaactcttgt
    15421 ataactatct tattagtttc aaatctatct gtgccaccca atctaataga atttgtgctc
    15481 ttaactaatt cgtcgctgat tatttcacta gaaccaagcg cataactctt taagcctgtc
    15541 gtgtcaaatc ccacactatt tccatctata agtattatag gtactccatc tctagcagct
    15601 actggtgatg cacttatcgc atctgcttct cctgtaaatc cattggctat aattacttta
    15661 tctattggct taatttcttt gattttttca gctattttta tacttgtatc aaatctattt
    15721 tcaccaaata ttctttgtac aaccttcttt ttggataata actgattctc aacattttta
    15781 cttattgttg attctgagcc tattatatac acagtctttg ctttgtttaa tctttccata
    15841 gttgtgtttg gaatctgatt ttgctttgtc attaaaatag gcgcatttaa ggctccagct
    15901 aaaccacttg cacttagacc atctgctaag ctattatctg agttgattag tattgctgta
    15961 ttgtagtttt gcttatctgc tatttttgct gctgtctcat atctgtcatt cccttttatt
    16021 tgctcaactg atgtcaacgc atttaccata ggagtattta ccattactaa aaatagtgta
    16081 agtcctatag ctaatatctt tttactattc ttcatattat tttctacccc caatgtactt
    16141 ttcatatttt ttattagtac tcatacttat ttttaatcaa aagttacaat tttgttacca
    16201 tattttatat atgataccat agttatccat acaactcaaa caatatacct aaagttttta
    16261 ttctctataa attcaaaaca ttataattta ttttatttaa ttaagttata agttcaaaat
    16321 aatttatgtt agtaatatgt ttaatcatta taagaagtat ctttttataa taatttttgc
    16381 aatatgaatt tctccacatt ataatcaaaa aataaaataa ggtatctcaa tctaattcta
    16441 aatgagacac cttattttta ttattttatc ttgttatttg tatattttat aatatctaca
    16501 ctatgtctta catattaacg cattgtataa tcatatatta tctaaataaa cttttaatat
    16561 taattcctat atcttattag ttcttagtaa ataagtttat tttttctgct attttttcaa
    16621 tagcgttttt agctattcct tcacctattt gtactaactt agtagcattc ttaagtctta
    16681 actcaactac ttcttcttgt gatttagaaa gttcttctgt agttaatact attggagcct
    16741 ttgaactagc tgcaagtggt gctgctgtaa gtgcatcaac taattgtcca tttccaccta
    16801 cataaccatc ttttgctaca tatgctttag ttgcacctgc atagtattta ttgattactt
    16861 tagcattagt atcttttcta ttttctccag ctaatctttc aactttatta tttaaatcta
    16921 tatcaactaa tttgtcttca acttcttttg atacagaatt ttctccaccc actatttcta
    16981 attcttttcc ttttaataat ttttcagcat ttgcatctaa ttcgttacct gtaactatta
    17041 ttggagcacc aaattgagca gctctagctg atatactcat agcatcagct tctccatttc
    17101 cacctactac aaaagcagtc ttaaatgtat cttttaattg ttttgctatt tctaaagaag
    17161 tttcaaatct atcttctcca gaaactctct caactttact tacacctaat ttagaaagtt
    17221 tttcttcagt ttcttttgat actttagatt ctccacctac tatatatatt ttcttagaag
    17281 atagattact tccaagtact cttaatattt cagcttctat ttctgatggt agtgaatctt
    17341 ttttagataa taatattggt gcattttttt gagaggcaag tggtgctgat gctaatccat
    17401 caactatagc atcttctcca actaaaatta tagcttctgc ttctccatct ttgaatctct
    17461 ctttactaac ttctactgct gttgtaaatc ttgaatcacc agataaaacc tctactttta
    17521 attcttttcc atcttttaca tcagttaata ctttgtgtaa gtctctaact aacttatggt
    17581 ttttacttga aacacttatt gtttctgact tttcatttgc cttgcttaaa actatatcaa
    17641 agcttgattt ttctggttta tctttatcat cactatcttc ttgttcttga gtaaggattg
    17701 cttcattttc aaatgttaaa gcatatccat ttttaacttt attaactatt tctcttccta
    17761 atgtagttaa tctaattcca tcaaataatt cttctgctga tatgtcttgt aaatctgcgt
    17821 ttttaactgt tatcgtatgt aattcttttg aatctatgtc actataactc ttttcataac
    17881 cttcaaaagt accttcttct gatgttatca cttttgagaa atctaaatgt tggtctccta
    17941 catttaatgt aataacattt gctgaatctt ttgctctttt tacttcaact gtatttgtag
    18001 attttatagt tgctgttaac ttgttatcag aatcttctgc taaagttgca ttgtaagcat
    18061 taactgcatc tactatttct tgagcagttt tgtagttttc aattttatag tcaactactt
    18121 ttccacctaa cacttgatgt cctttatctt gaatagtaac ttttaaactt tgcccttctg
    18181 ttaaagagtt tattttattt tgtaattgag ttgcattagt taagtttaca ttgtctactt
    18241 ttatatcgta aactctatca tttacatttg ctcctgcttt tgtatcttca aacttaactt
    18301 ctaatgcttt tctaacctct tcgattagtt tagcagaatc tttcgcactt actgtgtaat
    18361 ttttatcaac attttctgtc acagtattgt ctgcgaatat tggtgctgct gcactcataa
    18421 ctgtagcacc tgccattact attgccatac ttctcttttt gttgcttaac atatttatgt
    18481 cctccgaata ttatttttta ttttttatat tcgtatttcc cacacataaa taatattata
    18541 tataatatta tttttcaata tattttatgt gtttacataa tttactaaca caaacactaa
    18601 taatatcttc ttgaacccct tccccctact tagctctcta taattatgct caaagataga
    18661 aatccctatt ttatagtgat tttctactaa acaatcatat actaacaaaa gtattcaaac
    18721 tccattttta tatactacta tgcactaatt tttgctttgc tcttattact taaatatata
    18781 tcactaaatt aatatctacc tccatatatt aaaatatata acatattata aaatcatcat
    18841 aaattagagt ttaaaattaa aaattattct aatacatgta attgataaaa aataaaatat
    18901 aagagtagct actttaaaat aaatgctctt atatgattag atatttttgc ataattaaac
    18961 gataagaaat caaatttaat tttacataag tagaatatat atttttatat aataattgaa
    19021 aataaaagag gatacctcat aatgataaaa gaatcacttt gagataccct cttacaaaca
    19081 actaaatcaa taattttcta tctttcacac tcttgatttg caactgtaca agaagtttta
    19141 caagctgatt gacaagaagt ttgacactct ccacatccac cttttgcagc actttgcttt
    19201 aaagtagctt gagataaagt ttttacatgt ttattttcca ttttcaacgc ctccatttct
    19261 aattgtttcc tattgattat atcataaaac tactttaata taaactctat ttaaagttga
    19321 cacccaatac gcctccaatg cctcctgctg ctgatattag caataactta taaatcatcc
    19381 ctacttcaaa tacaaacttt tcttgtgcta aaaacacagt caaaaaaata catactatgt
    19441 acaaaagtcc tactaaaagt ccatacataa gtcctttttc tttcatgtgt tttgaagtgt
    19501 aaaaccctcc tatagctgca gaaatagtag ttataaatga tgagaccata gttatattat
    19561 ctccaccaat atctgtaaat gtcaaaaata agttatacac taataaaaca gccaaagtta
    19621 ttatatatgc atatcctaga cctttaaaaa tataattaga ttttcccata aaaaaacctc
    19681 ctctcaaatt aatatatatt cgagaaaagg cttttaaatt actaatttag tcttcttcta
    19741 tttcttcttt tttagattta acagttccta tagcccattt ttcaaatgga actttagttc
    19801 tatttggtcc taattctaat attacaacat catcttcaac tttagctaca ttagctacta
    19861 ttccacctat tgtaatgacc ttgtctccta cacttaggct acttctcatt tcttttaatt
    19921 gtttgtcttt tttcttttga ggtctaatca ttaggaaata aaagattgcg attactacta
    19981 cccatagacc tatactcata attatttgtt gctgtggcat taatttttca ctcctttgta
    20041 aattatactc ttttgtaaat cataataaat gtttatataa caatttactt tctctatctt
    20101 tgagtactat tgaatattat atattaaaat gattataatt aatagtgcaa aacctatatt
    20161 tcatatccat attttgcaaa aaattcattt ctaaaatcaa gtaatctatc ttccattatc
    20221 gcttgtctta tttgtttcat taaatttaat aaaaagtgaa gattatgagt agttattaat
    20281 cttgcaccca gtatttcatt tgcttttata aggtgtctta tataagctct tgaatagttt
    20341 ttacaagcat aacaatcaca ttcagggtca agtggagtaa agtcttcagc ataagttgca
    20401 tttctaacta ctactttacc ttggctagtc attgcagttc catttctagc gatacgagta
    20461 ggtaggacac aatcaaacat gtctactcct cttataacac cttccactaa gtcatctgga
    20521 ctaccaactc ccattaggta tcttggttta tcctgtggca taagtggagt tgtatgctct
    20581 agcacgtcat acataagtgg ttttggttct ccaacactaa gcccaccaat agcatatcct
    20641 ggtaaatcaa tactagttat ttcttttgct gattgttctc ttaagtcttt atacatccca
    20701 ccttgtatta taccaaataa agcttgtttg tcggtattat tatgggcatc tttacatctt
    20761 tttaaccatc ttgttgttct ttctaaagaa ttttttacat attctctatc tgatgggtat
    20821 ggtgcacact catcaaacgc catcattatg tctgaaccca atgcattttg tatttccata
    20881 gctttttctg gagttagaaa atgttttgaa ccatctaagt gtgacctaaa ttctacacct
    20941 tcttctttta tctttcttaa aggccctaag ctaaatactt ggaaccctcc actatctgta
    21001 agtattggtt tatcccagtt cataaactta tgaagaccac cagctctttt tataagttca
    21061 tgacctggtc tcatatataa atggtatgtg ttactaagta ttatttgtga accaatctct
    21121 tttaattctt ctggtgtcat agattttaca gttgcttgag ttccaactgg catgaatatt
    21181 ggtgtttcaa taatcccatg tggagtgtgt aatcttccta accttgcacc actctgctta
    21241 caagtcttta tcaattcata tcttactgcg tacataaagt tctccttaac catatttatt
    21301 tctcattatt tcttaagata tatttgtaat aagctattca tataatttat tgtctaaata
    21361 gatttatctt cgtcttcttt gttctgttgc tccttaattg cttcttcatc atgaatactc
    21421 attatattaa gagcactctt gtcttttgtt actaaaaact tttttatagc aaatagtcct
    21481 gctatagcta taactcctat taaaacatct atcatacctt catttttagg cagtagcaac
    21541 atttttctag ctattgcgta aagtaatgcc tcaataaatg ctcctggtat atgaagagat
    21601 agcattacta caagttcaac acctattact aaaagtaata tttgtgctaa aaaatcatta
    21661 agctgactgt attgtactgg attttgaaaa tcaactatat atgctcccca cataagtctt
    21721 agaacatcta tagttcctaa aaatactgct attagaacta ctactgctaa aacacgttca
    21781 aatataaaag caatctttaa tatagggtta ttcttttttt gtttttgtaa actatgcatt
    21841 cattacctcc ctactttata atcatagcat ctccaaaact gaagaatcta tatctttctt
    21901 ttactgcctc attatatgca tttaaaacat tatcctttcc acaaatagca cttactaaca
    21961 ttattagtgt agattctggt aaatgaaaat tagtaataag tttatctaaa actttaaatt
    22021 tataacctgg atatataaat atttctgtcc atccactagt ttctttcatc tttccatcct
    22081 cattacaagc tgattctata gttctacaac tagtagtacc aacacatatt acattgtttc
    22141 cattttcctt cgctctattt attttgtcag ctgcttcttg gctaaccata taatattctg
    22201 aatgcatctt gtgatttaaa acatcttcta cttttactgg tctaaaagtg cctaaaccaa
    22261 cgtgtagagt tacaaaagct atatctacac ctttttcttt tattttattt aacaattctt
    22321 ctgtaaaatg taatcctgct gtaggtgctg ctgctgaacc attatgcttt gaataaacag
    22381 tctggtatct ttctttttca tctagtctag cagtgatata tggaggtagt ggcatatttc
    22441 caagttcatc caatatttct tcaaaaattc catcataaag gaattttata attcttgaac
    22501 cttcatctga taaatctaca acttctccaa ttagctttcc atttccaaaa gaaaatttag
    22561 tccctacttt tgctcttttt cctggtttta ctaaagcctg ccaagtatct tcctctgttc
    22621 tttttaataa taaaaactct attttacctc cagtttcaag cttctctcct attaatctag
    22681 ctggtataac tctagtatta tttaatacta aacaatctcc tggatttaaa tattctatta
    22741 tatctctaaa tactttatgt tcaatattac ctgtctcttt atctaatacc atcaatcttg
    22801 agctagctct atcttctata ggaacttgag ctataagttc ctgtggtaag tcaaatttaa
    22861 aatcacttgt tttcaatgtt aaacttcctc tcttaaaatt tctccagcta caataatatc
    22921 aaaaaaacaa cttattttcc acaatattac cataatctat caagaaattg ctatttttaa
    22981 cagacaccat atccacaata tatagatatt tttccacgat atacatgtct tatccacaat
    23041 atatagatac aatacacaca atttttatcg tgtttatcca cagtatctac agatataatt
    23101 accattttgt ggatatattt gaatttttag ccattttaga aatattttaa tttaatgact
    23161 cttttatttt ataaatactc gtataatcta tttttatttt tcattaggat atggaatttt
    23221 taagtgttca tatgctaatg gcatggctac tcttcctcta ggacctctat ttataaatcc
    23281 taattgaagc aaatatggct catatacatc ttctattgta tttctatctt ctccaataga
    23341 agctgccaat gtatctaaac ccactggtcc tcctctaaat ttttctatta tagtcataag
    23401 aagcttctca tctacaaaat cgagacccaa actatcgact cctaaaagtt ccagagcatc
    23461 ttttgccacc ttatcagtta tttttccatc agctctgact tgtgcaaaat ctctaactct
    23521 ttttagtaat ctatttgcta tccttggagt ccctcttgaa cgttttgcaa gttctaatgc
    23581 tccattagac tgtatctctg catctaagat gcttgaagac ctaagtacta tttttgaaag
    23641 ttcatcaact gtataataat ccaatttaca aataacacca aatctatctc taagtggatt
    23701 tgtaagcata ccagctcttg tagtagctcc aataagtgta aatttaggaa ggtcaagtct
    23761 tatgcttcta gcagatggcc ctttccctat tattatatct aggcaaaaat cctccattgc
    23821 tggatataaa acttcttcaa cacttctatt gattctatga atctcatcta taaataaaac
    23881 atcattttca tttaaattcg taagtatggc tgccaaatct ccagctcttt ctattgcggg
    23941 tcctgacgta attcttaaat ttacccccat ctcatttgct attatacttg ctagtgtagt
    24001 ttttccaaga cctggtggtc catataaaag cacatggtct aactgctcat ttctcgattt
    24061 agctgcttct ataaatatac taagttgttc tttggatttt tcttgaccca aataatcttc
    24121 taaagttttt ggtcttaaac tgttttctat atctatatct tccatcttca ttgtagaagt
    24181 tattatccta ttttcatctt caaaaccttg catttatacc cctcctctcg tacttttgaa
    24241 aatctacttt atatattttc aaatttacat aaactcctta aatattgttt taaaatccaa
    24301 atattataaa atccaaatac tatttcaagg atttactcat tatatatgta agagctttct
    24361 ttataatagc ttctgtattc ataccatctt ttttacattt ttgaactgcc tcttttgatt
    24421 ccgacaaagt atagcctaaa gctacaagtg catctacaga ctcatcttgt gatatcaagg
    24481 taggtttttg tgataataac gttggttcaa actctatgtt atttttgtct accttatcct
    24541 taagctctaa gacgattctt tctgctgtct ttttaccaac accaggagct ttggataatt
    24601 ttccaatgtc ttcacttaaa atatatgctc ctaactgtgc tgatgatgca aatgatagta
    24661 ttgaaagcgc caccttaggt cctattttag atacagatgt tagtaattca aacatcttaa
    24721 gttcttccct acttacaaaa ccacaaagac tcatatcatc ttctcttaca attaatttag
    24781 tatatatttt tgtggcttct cccacttgta aattcattat tgtattggat gatacattta
    24841 ttttatatcc tatcccatta ttttcaacaa ctatactgtc tatataaatt tcttctactg
    24901 taccttttat ataactatac atacttacca cctatattct ttagagtttt ttcaagtttg
    24961 tttgaatgag catggcaaat agccacagct aaagcatctg caacatcatc tggttttgga
    25021 acttttttta gacttaaaaa tgaagttacc atctgttgaa cttgtgcctt atctgctctc
    25081 ccatatccaa ctacaccttg ttttacttga agtggagtgt attcatatac aggcttccca
    25141 ttatgagcac atgcaagcat agtaacacct ctagcttgtg caactgttat agctgttttt
    25201 acattcttgt taaagaataa ttcttccatt ccaacttcat ctatattgta attctttact
    25261 attgtatcaa ttcctttata cacaagttcc aatcttctcg atatattcat atgggcaggt
    25321 gttgtaactg ctccataatc gattacttta aacttgctat ttttgtattc aattatacca
    25381 tatccaacta tggctatacc tgggtcaatc cccaatatta tcatttttta aaattccccc
    25441 taaaaataaa agaaatacca aaatgtattt ctcataaaaa cttatgttct aaatcattat
    25501 atcataaata atattaacaa atcataaaat gctctatgta aaatataaaa tcaaataaaa
    25561 aagttatatc aaagcaagat tttacctcaa tataactttt atatttatta caaataaatt
    25621 attgtatttt tataggaatt aaatgtgact acaattttaa tactaatcta tttaatatta
    25681 aattatttat tattaactta cttagtattt aacttgaaat aatattaatt tactattaac
    25741 ttgcaaagtc ttctaatctt gaataggcct cttcttcagt atccataact acaccttttt
    25801 tgatttcatc ttgaactgtt ttaggaagtg aatctactct tatttttgtt ttttctacta
    25861 atgttttact tccactatca tcatatttat atagtgctat atttccatta tcattttcaa
    25921 tagtatattt accttttata gtagcgttca ttgcttgtac atctttatta tcactatatt
    25981 tagattctac caatatgatt tcatttttat ccatactctt tattttttta gttggatatt
    26041 cttttttaaa atatgatact atttctgatt ttgtcttatc taataatgac tctggaacta
    26101 tcccagccat tgtaagtgtt ctcttatagt tagtttcatt ttcgccttca ctttgaagcc
    26161 aaacttcaca atttttattt aatccaaaag cttcttttga cttttcgtcc atattcgcac
    26221 tagcacttac aactttacta gtttgaactc cattattttt taatcttgtc cctatataaa
    26281 ctccgctact taatacaaat actccaacca gtattaaggc agatattttc caaggaaata
    26341 attctttttt cttcttttca aacattgatt acgcctcctt tttctattat tttctccaag
    26401 ctgtaagttt ttatacaatt taggtaaatt ttagaagaaa atatatttat atttcaaatt
    26461 actctaagaa tagatatttt ctaaaaggta gttttctctt aatggttata ttcttgtaat
    26521 tatatggaca taatttataa taaataatcg tttaggaggt aataactttg gaaagaataa
    26581 actgtaatgt aattaactgt tcacataaca acagccatat atgttatgca gataaaattt
    26641 ttataaatgg tgaaaaagct cacaatgaca gacatacttg ttgtagttca tttttagatg
    26701 aatctattta tagtaattta tctagcaacg ttaataatag aaattgtgag gaattagttt
    26761 gtactgtaaa aaattgtaaa tacaacaaag atggtagctt atgtacactt gataatatat
    26821 tggttgctcc tgaaactgat agagtaaacc tttatattga aacatgttgt tctagtttcg
    26881 aatgtaaata gtcatagtat aaatataagg gtgtcaaatg taattaaact aacatagtgt
    26941 agagtatcta caactattag tttgattata cttgacaccc ttatttattt taaataaaat
    27001 ttcattatta taaccatgtt tataatgttc taactgtaat taaatttgat aaatttctat
    27061 aaattggtat aatcaatatg taaattgaat ttagcaaatt aatcataaaa ataaaatatc
    27121 gagtatatag tgttatacta actagaaatg aggtgcttat gagtacattt tatattcaat
    27181 ttctttattt tgtaatttat agctttatag gatggtgttg tgaaactaca tattgttcag
    27241 ttttacaaaa acaatttgta aatagaggtt ttttaaatgg tccattttgc cctatatatg
    27301 gatttggagc attagcaata gttgctacct tgactccttt tgtacacaat attcctttgt
    27361 tatttttgtt tagtataatt ataactagca taatggagta ttgtacgagc tttattttag
    27421 aaaaaatatt taatatgact tggtgggatt attctaaaca taaatttaat attcatggaa
    27481 gagtttgtct ggaaaactca cttatgtttg gtgtattatc attgattgta atgcttatag
    27541 tacatcctat tgtggttgac tttattaact ctatctctaa aaatgtgcta tttatatttg
    27601 ctatatctat agaaatttat tttgttttag atttagttat cacagtccat acaattttac
    27661 aattaaatgg taagttaaaa caaatcaatc ttataattaa ggaattaaaa gataagaaag
    27721 aatactacaa acttattaca caagaaacta tagaaaacaa acttgaatct ttagttgaaa
    27781 atagactgga tactttggaa gatagatttg aaatacttaa ggaaaaagaa aactatcgct
    27841 atgctaaaaa tcgtattgat gagctaaaaa ataaacttaa tactttgctg tctaaccaca
    27901 aattacttca tagacgtata atcaaagcat tccccaacat aagctctaat aagcatacag
    27961 atattttaaa taaaatcaaa gaaaatatta aatcaattaa aaaagataaa taataaaata
    28021 aacacataag ttaaataata aaaatactaa tacataagat taaggtttag aatatttttt
    28081 gtaaaatact ctaaacccta attaatatac tattattttt aatcttaatt tataatttta
    28141 gtttatttta cttttaacac agtaaattta agcgtttaaa cttcaataca ctaatatact
    28201 accccatcaa tatctcaact gattctctaa gtgtgctaat ctctcccaca tttccaggga
    28261 aaattatata tggcatatta ggaaatttgc tttctttgcc tgtcatccat actggtatac
    28321 caggttttat ctgtcccatt actgtagctt tttttactct tagtgcttta gtaccaacat
    28381 cactagaagt aattccaccc ttagctataa taaaatttgg tcttacatta agcataccaa
    28441 ttatactggt aactgcatct gatatttcta cagaaatcat aagctgttta tctttatcat
    28501 tcgtatccaa gtcaaaacgc tctcgtctag tataaacagc aacagtttta ccactgctta
    28561 ttttttcctc tacaactttt ataactcttt ttacctcatt ttttagacca ttttcctgaa
    28621 gaactaaatg ttgattaaat tctataaatt ctattggata tttacaattt ttaagttctt
    28681 ctaactgttg ggtagtctta ttcacatgtg aaccaacaag tataattcct ccattggtat
    28741 taccttttga tactaactca tccttagtaa gcatttcctt atcactcact ccgccaagta
    28801 cttttgttat ggcagcagcg cttctaaaca taaattcctt accactattt actgctcgta
    28861 taaacgcaat tgtaaatacc tttacatcta cataatcggt tgcatttaca ataattttat
    28921 tgaatccttc agctttttta agtttttctg ttatagcttc aatatttagc tctctaagtt
    28981 ctttagaaga aatatatatc atatcactag atttgtactt tccatgtgtc ttttcttcac
    29041 accactcacc tatatcagat gatttatatc caaaagattt atcctttgca aattcagtca
    29101 ttcctgctgg tacaagtatt tctccttctt taacataatg tacattatct atagtaaacc
    29161 taccaccttc tttaaaaaat ggcattatta tttcaccctg aaataacttc cccgataatc
    29221 tctctacttc gctttttaat aaattagttt caacaggata atgtcctctc atagtagaat
    29281 cactacgact tatgattata aaatccttat taactttctt tgatacatcc acaatattct
    29341 ttgaaatttc tttatgtgct tttgtagttt gagcaacagt aaatcccctt gaattagtaa
    29401 gtataaaaaa catactattt ttctcattaa aaccttgttc catactatcc ttatcccaat
    29461 cagtataaac agaaatatca tgaactgttt gtacacctgt tgggtcatca tctaatacaa
    29521 caatttttct atctaaatgc ttaagctcat ctgcaagaag ttcatttaat ctgtcctcat
    29581 ctacttgttt aaaagtatta agtatatcta tgctaattgg ttttacttct ctcatttaaa
    29641 acgccccttt atgtaatttg tatagtctaa atgtaattta tataatataa acaatagttt
    29701 gttatttata gacctggaag tggtataatt ccatttataa cactaagcaa taaaaccatt
    29761 ataaatgcta caactccagc aatggttcct ccaattgtcc aagagtttaa agtatcttta
    29821 actgataatc cactaaatct tgaaagtacc cagaatccag aatcatttgg aagggagaaa
    29881 ccaactcctc cagcacatat agcaagccct actaaaactg gtgacattcc agttgccaaa
    29941 attgtaggtc ctaaaattga tgatgttgtt actaaggcta cagtagcaga accttgagaa
    30001 attctaagca aagcacttaa taagaatcca agcactacaa ctgatatact aagaccagac
    30061 atagtatcaa ctaagaagtt acctatacca cttgcattta taacactgcc aaacgcacca
    30121 cctgaacctg ttatcaataa tattagccct gctgaatcag cagcttcaat aactacatct
    30181 cccataggtt tatttatgta tctctttaaa aatcctattg ccaccaatat tccaagaagt
    30241 attgctatgt ttttatctcc tataaatgca agtactccac tcattgtaga acctttttct
    30301 aagaacatac taaatacatt tgatataagt ataagaagta ttgggaacaa taaagaaaac
    30361 atagaaattc caaaacctgg tactttagaa gaatcatcat cttcaatttt tatgctatct
    30421 tttccctttt ctaaattttt ttctctatca ttctcatcaa tagcataagc tggatatttt
    30481 tcaaaacgtt ttccataaat ccatccacct ataagaactg ctggaaaagc aacaattata
    30541 gaataaagta taaaactacc tacatttgca tttacatttg ctgcaactgc taaaggtcct
    30601 ggtgtaggta ttacaagtgc atgacctact atagtcccaa ctcctaaggc acatacaaaa
    30661 accattagag atttcttagt tactctcgaa acatacttaa ttataggcat cattataaca
    30721 aaagctgcat ccataaaaac tggaattgaa attaaaaaac ctgctgtagt tactgctaaa
    30781 gcactgtttt tttctccagt cttagctaac aacccctttg caatagattc tgttgctctt
    30841 gattctgaaa gaatatttcc aaatattatt ccaagtccaa ttacaatacc tatactacct
    30901 aatgtatttc caaatccagt cgatattgtg gttgatattt catttatagg cattctaacc
    30961 ataaagcctg taattataga tgttatcagt aatgccaaaa acggatttac tttaaattta
    31021 ataatcgata caagtaaaat tcctatagat ataacaaatg ctatcaaaag tccatatcct
    31081 gtaatcataa tctttacccc tttatcattc tactttatta tttatcaatt ttatttttct
    31141 tttactatga taacttaacc tcgctgtatt tatataatct aatcctaata atatgtattt
    31201 aattatattg atatatttaa atattaatta ttgtgttttt ttatttcaac acctgccaac
    31261 ttttcaaaat actgtgctat tccactatgg tcatcttcca aatgtccatg tacttttaat
    31321 gtctgcataa tttctaataa ttgacttgtc ataggtagcg gtacatctaa attgtgtgct
    31381 gtttgcatta cattcttaat atctttcaaa ttaatagata tttttccacc tggtttaaaa
    31441 tcacgattca taatcattgg tatcttcgca tccaatattg tactaccagc taggcctcct
    31501 cttattgcct tatatacttt ttctgggtct gcacctgctt ttgctgcaag tacaaatgcc
    31561 tcagaaactg ctgctatcgt caaattaact attacttggt ttgtaagttt tgtaacactt
    31621 ccactaccat tatctccaat taaaatagat gagcttccca ttatattaaa aaatggctct
    31681 actttcttat aaatttcttc cttaccacca gccataaaag caagtgtacc atcaatcgct
    31741 tttggctccc ctccacttac tggtgagtct ataaattcta agcccatatc ttttaattta
    31801 tttgcacaca atattgattc tgtaggagta actgaactca tatcgactac tatacttcct
    31861 tcttttaaag tttttgccaa tccatccatc ccaaataata tatcctgaac aatagttcca
    31921 tttggaagga ttgtaaatac aatatcacat tcttgaccca tttctaaaac acttgcgtat
    31981 tttgctccac atgatataag ttcatccaca gcagatttat taatgtcata caccaaaaga
    32041 ttacatccat cttttaataa attttttgcc attggctttc ccattattcc taatccaata
    32101 aatcccaatt tcattttata accctccata tcttcacatt tttgaaaacg ttttttatgt
    32161 tttagagtat actacatacc agtataccgg tcaatataaa atataaaagt atttatttta
    32221 acaataaata gtaattttta aagtcattta ttatatttag tcatcctata taagttttaa
    32281 tacatactat tgtttttaaa tataatatgc tacttttaca gttttgtatt tttactaatt
    32341 tcaattagtt ttaattaatc tgcctttaat gttaatcata cttcacactt caataatttt
    32401 ttatattttt taatcttatc tgcaatttaa attgcaaaag attgacctta ttttcactat
    32461 gttgtatgct taaaattata taagtttttg tatttttata ttgtttaggg gggtattatt
    32521 aagtatggct acatttaaaa gattacaaga acaagcatat gactacttaa aagaattgat
    32581 actatctgga gaaatgatag aaaatgaaat atattctgaa acaaaacttg ctagtgaaat
    32641 aggtatctct agaacaccta taagagatgc tcttcaaaga cttagtcaag atggatttat
    32701 agatataatc ccaagcaaag gatttagaat ccatcaaatt acagcaaatg aaattgtaga
    32761 gatattccaa atacgctctg caatagaagg gttttgtact tttttaatta caagtcaata
    32821 caaagaagct agggcagtag aaactataag taaattaaaa catcttttag ataaacaaaa
    32881 agatatttta ctaggtgata aaaatttaaa ctcatttgca gaatacgata cattatttca
    32941 cacaactata gtttcatatg ctcaaaatac tgaatttgac aaaatgttta acaactatat
    33001 gtacagaata aaaaaattag ctctcgattc attatcacat gaaggaaggc ttgaaacaac
    33061 attaaaagaa cacacagata tttttaataa tattgcaaat ggatgtattg aagatgttta
    33121 caaaactact ctcattcata tggaaactcc taaatacatt aatttagaag atttttgtaa
    33181 caataagtga ttttagtatt atactttcat ttattaaaaa tatatctacc tcaaattaat
    33241 ttcctaattt ataaaaaata ttctataatt atattgtaac taatactatt ctaaggagtg
    33301 atttttttga acgaaaatgt tgtaactaat gaaactattg acaaagatta tgctattgaa
    33361 gaagttcgta tggcatgcaa acattttggt gacttatatt tttatttttc caaagtgtta
    33421 tttgaagagt ttggtgaaga taaaactatc gaaatactaa gaaaagtttt atttgaaagg
    33481 tctgaagaaa gagctattgc tatgagagaa agagctcttg aaaatggaga tgaattaatt
    33541 gcagacaata ttattagcac aactgatgtt ccatttctag gatgggttcc tgaatttaaa
    33601 gagttacatt gcccttatgg agcttcctgg cttttaagat ttgaggaaaa cccttggttt
    33661 aaaaaattcg cttctcttta ctgtgatgtt acagatacta ctgtagctga agtatttaca
    33721 ggagatacgt cccataaaat aacaaaaaac attctatggg gagataaaag ttgtgaaaga
    33781 atatatttcc atgatgacaa ggtatctgaa ggtaaatata cttatggaaa taaaaactgt
    33841 aaataaataa tttaaatttt ctatattcat atactatatt ttgtactttt tcatataaat
    33901 attataacta gtcttttatt ttacttttgt ttaacctaaa gataatttac ttttaatcta
    33961 tatgaattat catataaata atcagaaagt tatctttata aattatagtt ttagatttat
    34021 aatttataaa atatttatgt tctttgtaaa attttttaga agcttctaat gttatgctat
    34081 aaaactatct tgagaagggc tgattgaatg tttaataaaa tatatgaaaa gaaagaattt
    34141 atagtttttc aagttaaaaa aggttatgta gtttataata ctagaaagag ttttgaagaa
    34201 ggtcatacac atttaaagca ttttgaagca gcaaagacag ctattgattt agcaataaat
    34261 aaaaagattc ctagaagcaa agatggatat tatctgacta gtttaattcg cataagtgat
    34321 gatggttatt acattgataa gttaagcgaa ttgttatatg ttagagaaca aaaaggtaaa
    34381 aaagaaaagt actgtaattc tggttatcag atgtaacaag actagttgtg aattataagt
    34441 accaatcata aataccttta gtcttaaaat caaagagtat cagtaaatct ataaaaaaac
    34501 aacttactga tactcttttt aattatatga ttaatatttg aatttgatac aaaattttat
    34561 acaaaagttt atgttatcta ttctgattct ttgtctgcaa attggctatt ataaagcttc
    34621 tcataaaatc cacccttttc cattaattca tcatgattcc cctgctctat aatacttcca
    34681 ttattcataa ctaaaatcaa gtcagcattg cgaattgtag aaagtctatg tgctataaca
    34741 aaactagttc taccattcat aagatttctc attgcttttt gaagcataag ttccagccta
    34801 gtatcaacag aactagtagc ttcatcaagt atcaatattg aagggtctga tataaaagct
    34861 ctagctatgg ttaaaagctg tttttcccct agagatatat tagatgcttc ttcatttaag
    34921 aacatattgt atccatctgg aagagtggtt ataaagtgat gaacatttgc tacttttgct
    34981 gcttgtataa tttcttcttt agtagctcca aatctaccat attctatatt ttcaaaaatg
    35041 gttccattaa ataaccatgt atcttgaagc accataccaa acattgaacg caaatcttta
    35101 cgtttcatat ctcttatatc tactccatca attttaatag cacctttctt tacatcataa
    35161 aacctcatca ataagtttat taatgttgtt ttacctgctc cagtaggtcc aacaatagct
    35221 accatctgac cactttttac ttctgcattt aaatcttcta ttaaagttga attttctcca
    35281 tatccaaaat taacatgctc aaatgtaaca tttcctttta cattttctat cttaactgga
    35341 ttttcaacat caggtatttc ctcttcttcg tctaaaattt caaatacacg ttctatagct
    35401 gcaaaagaag attgtattac tgtagataac tgagtcattt gtgaaagtgg ctgatttatc
    35461 tgccatatat aacgcacaaa tgcttgtaaa tttcctactg ttatccctcc acttactgca
    35521 tatatagaac cgacaactgc cacagctgca attccaaagt aagttactaa tgaaaccata
    35581 ggattcatca tacttgatat aaattgtgcc ttaaatccat tttcacaaag ttcttgattt
    35641 acttttttaa attcttttat agaatcttct tctttaccat atagttttat ttcattaaat
    35701 ccagtataca tttcttgtac ttttccattt aattttccaa gagcattttg ttgcaatgaa
    35761 aatctacttt gagatttttt tactacaaat ttagatacaa aataactaat tggaattata
    35821 aaaagagcta taagagccat atatatatta attgtaaaca tcattgaaag tgccaaaatt
    35881 atgactaaga ttgagtttac aatttgcata aaactctgtt gcaatgcatt tgatattgta
    35941 tctacatcat ttgatatacg acttaataca tctccaaaac tattactatc aaaataacta
    36001 ataggcaatc ttcttatttt attttcgact tcatttctta aatctctcat tgtgttttga
    36061 atagcattcg taagtagaaa acttgaagta tacgtaaaaa ttgcattagc aaaatatata
    36121 catgctaaaa tcaccaatat ttttgttaca taatcaaaat ttacggaagc ccctggtact
    36181 ccttttgcga tactaattac atctttagtc aattgtgtta ttatcaatcc ttcagtttta
    36241 ggtgcaagtg cattcatagt agctgctgaa attatacata taatagctat aataaaggaa
    36301 aatttatatt tctttacaaa tggaagaagt ctaccaattg ttttcttaaa acctttagac
    36361 tttttcatta ttctaattcc tcctttttta actgagatgt tgcaatttca tagtatattt
    36421 cacagttttt taataattct ttatgagttc ccattccgac aacttgtcct tcattgagta
    36481 ctataatctt atcagcatca ataatagaac taattctctg ggcaacaatc aatacaattg
    36541 catcttttgt ttccttcttc aatttagcac gaagtattgc atccgtttta aagtcaagtg
    36601 cagaaaaact atcatcaaaa atgtatattt caggtcttct tataatagcc cttgcaatag
    36661 aaagtctttg tttttgacct cctgatacat ttgctccacc ttcactaatc aattcatcaa
    36721 actttctagg tttttttgat ataaactcat atgcttgtgc aactcttgcg gaatattcta
    36781 attctgactc tcctgcctta tgtttcccaa atctaatatt atttgctatg ctaccagtaa
    36841 aaagcagtga tttttgaggt atgaatccta tcttttttct taaagctttt aaatcatatt
    36901 ctcttatatc tactccattt atcttaatgc taccctctgt tacatcgtaa aatctaggta
    36961 tcaagttaat tagagtactt ttcccactac cagtacttcc tataaaagcg actgtttcac
    37021 ctgtttttgc agtaaatgaa atatccttaa gtacagaagc ttctccatct ggataggtaa
    37081 aagttacatt atcaaattct atgataccct tattttcagt atccttaatt ccattttcag
    37141 gattttttat caatggttct tcatttaaaa gttcttttat tctatttgca gatacctctg
    37201 ctcttggata cataataaat accattgaaa aaagcataat tgaaaacatg gcatggaaaa
    37261 gatattccaa aaatgcaacc aactgtccaa cttgaagctc tccaatattt atcttctcac
    37321 ttgaaatcca aaatacagcc aaaacagcta tatttaatag taaaaagaat gctggttgag
    37381 taattgacat aagcttaaat agtttcttag aaacattagc ataatatgta tttgtttttt
    37441 caaatcgctc tgtttcataa tcatcatttc caaaagctct aattactctt attccagtta
    37501 aattttctct cgatataagg tttaatttat ctagtccttt ttgttgtttt gaagatattg
    37561 cctgagatac ttttgctatt attgctactc caataattat aaaaggtact gatatagcca
    37621 atactataga aagttccaca cttgttctaa ttgtcattat tatacttatg atgaacataa
    37681 ctggagttaa taaggcagtc cttagtagta tattaacaaa ttgttgaatt tgaaatgcat
    37741 cattcgttgt tcttgtaatc attgaagaaa ttccaaattt attgtattca gtatgtgaaa
    37801 attcttgtga tttctcaaat atgtcatttc ttatatctct ggttacactt gttgatacct
    37861 tagcagaaca ataacccaac aatatactac ctacaacccc tataatagaa ataataacaa
    37921 tcactattcc catttgtttt atgtaagcta tattctgatt tgcaatacca ttgtcaatta
    37981 ttttagctat tatagttgga atccccaact cgactaatgc aaaactaaat atagccagta
    38041 tgtttaatac tattaataac ttataattct ttaaataact taaaattaat ttcatatttt
    38101 tctcctttct taatttttga gatagtgtaa tcatgtaata atactaaatt tataaatgct
    38161 taattttata aaaaaaggag aaaagagtta acgcactctc ttctccagca tattcttttt
    38221 aatacaccct ccgacaagtc gcttatatgt taacatattt gacaagtaat gtcaattcat
    38281 atagctaaaa gtcttttaaa tgcttcatct cataacattg taacaaattg gcatgtaatt
    38341 tatttttgtt ttttactgta ttccaatttt acaagttctc caatattact aactttctta
    38401 aaattcaaat tagtcttatc tataccagac ttaaatagtg aaatccctct ccccaatatt
    38461 attggcaata tatatatata ataatcatca attaagttat ctttcataaa taattttact
    38521 atatcgcctc caccaactat ccaaatatct tttttatcct tcttttttcc aatttgctca
    38581 attaattttt taggttctaa attagtatac tttacattct ccagttgtcc cattaattgt
    38641 atttgagtat atatagcatt ccttaccctt atagtaccat tcatttgggc ttaattcatt
    38701 tgcaatctgt acatatgtgg aatttcccat aatgatagta tcaattgtat cgtaaaaagt
    38761 attataatca tcttcctgct cattcttaaa ttctgaagat atctcgtcta aaaaacttac
    38821 actgccattc tcttcagcta tataaccatc taaacttgtg gcaatataaa gtactacttt
    38881 tctcatgttt actacctcca acatttatag tgtgattatt ctatgtaaac atcacttttt
    38941 tactcattca ataataccct attaataaat cctcaaacta atttaaataa actttgcata
    39001 aaactaaaaa tatattatat aaataattta aaatcacact aacttattga aaattctaat
    39061 tattacaaat aaatattgct ctctgttaac gctagtttta taatataatt ataagctatt
    39121 aatattataa agccgatatt actaatataa taattatagt taatttactt ttgttgaaag
    39181 gtggtaaata acatgaaaat ttcagaagtc gctactccaa attttttgct agatttagac
    39241 cagttagaaa agaatataga taaaattcaa caaatttgta caaataataa taaacaactt
    39301 tggcctatgc ttaagacaca taaaagtact tacatagcga gacttcaaaa aaatgcaggt
    39361 gctagtggtt ttctagttgg aacacttgat gaaggtgaag ctctgataaa agcaggtttt
    39421 gaagatatta tgttggctta tccttacatg ggagaagtaa atataaatag aattgtgact
    39481 atggcatcaa aaataaattt aatctgtgct acagataata tagaatgtgc taaggcttat
    39541 agtgaagctt tttctaaatc aaaaatagag tgcaagctac ttattattgt agattgtggc
    39601 ttacaccgtt ttggggttga accagaaaaa gtggttgaac ttgctactga aatttctaag
    39661 ctagaaaaca tcaagattgt tggtataagt tcgcatccag gtcaagtgta cggttgtagt
    39721 tctccagaag gtgttccaga tgtttgtgtt caagaagatt cttctatgga gaaagcatac
    39781 aaagctctaa ctgataatgg atttaatata gaaattgtcg cttctggtag tactccaaca
    39841 gttgaagctg aagctgcttc taaaactata actgctgtaa gacctggaaa ctatgtatac
    39901 tatgatgcta tacagatagg tttaggttgt gctactgaag atatgtgtgc tcttactgtt
    39961 gtgtgtactg taatctcaaa aaatagcagt ggatattatt taattgattg tggaagtaag
    40021 tgccttggac ttgataaagg tgcacatgga aatagtagta ttgttggtta tggtagggta
    40081 tgtggacatc cagaacttac tattgattca ctttcagaag aagttggaaa aattaaagta
    40141 aatggtgaaa ctgatataaa aattggtgat agaataagaa ttatacctaa tcattcttgt
    40201 tccactgcaa acttgacgag taatttatta ggttttagag gagatacaat agaaaaagtc
    40261 atagaagtag atattcgtga aaattcaaaa caaatcatcc tttaaaaata tatttatttt
    40321 gatattaaca tcatgaaagt atatttttct taaaattatt gttaataact aatgttaatg
    40381 ttggataaat aaaaaacata aattctagaa atataaattt aaaaaaagag gctgtctcaa
    40441 aattggatat gtccctgtca agtagacaga cttaaaaaag gctatcttta tgcggaatga
    40501 gttcgatatt caatcggact cattccattt tttaa
//
LOCUS       DAELSY010000018        40531 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.018, whole genome shotgun sequence.
ACCESSION   DAELSY010000018 DAELSY010000000
VERSION     DAELSY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.018"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1439
                     /locus_tag="KPG09_000844"
     CDS             <1..1439
                     /locus_tag="KPG09_000844"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965469.1"
                     /translation="TLIDEVNTVKAEVTTAKNTMISEVTTAKETMQTEVTDAINAIPT
                     KEELKGVGIEIKGSLDNISALPVNPTLSDAYFVKSATIENQIDLYVWDNTNWVKVPDI
                     KIKGENGDGLEFNWDGTRLGIRIEGQENYTYTDLKGQKGDKGDSIEFNWNGTRLGIKI
                     EGQENYSYTELKGEQGYTPTIGENGNWWINNIDTQKPARGASLRILGKLDSIDNLPLD
                     PTIGDCWIIGRNIYIYQTKWEDLGSLAGVDGKNLEFNWDGTQLGVRQQYELDYKYIDL
                     KGDNIEFAWDGTRLGVRIEGQENYTYTDLKGQKGDSIEFSWDGTELGVRIEGQEDYSY
                     TNLKGATGNKLEFNWNGSQLGIREEGQTEYIYTELRGEQGYTPAIGENGNWFINGEDT
                     GKASKGKVTWNELLEKPKELDYIKKSTTFNSDGSITDILDSVSKTITKFNADGTIVDE
                     KYIDNVLVSKVKTTFKGNQIEEIKEEVS"
     gene            1439..3289
                     /locus_tag="KPG09_000845"
     CDS             1439..3289
                     /locus_tag="KPG09_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016040100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG0965470.1"
                     /translation="MSWAETYKVNSDLQGEPLNFLSYLQDIKLNGLDSYVLFIGNARI
                     WEELYLNSLYLFSDRGIRETVYTAFSETDIDNLFNKSTKLGEQLNAFYRTDIFSLGNA
                     DNVVKEMTIEHYNSLEEKFKAGYDRYVTREQEKSTIGAWFNSTFSLDNTDLENLTTIE
                     EILANVEATNAILNNSNAIVALTMCKSSMDAVVASSNAMDLLGQYILRVTTESPVIRA
                     ILKNNVIRDAIINSDEAMTQISSNENSVMEIFNDLEATKVLVQNQNSINKILTNNVTV
                     EKIIPNLLEMKYNLQTSLNYINTIKSNIASGKGQIMAITYNEEIFPILKNAVKNYDGM
                     ETTRNISQRDIEEKIKISDAILESSIAMATFANNSIIVNKVGDRVGIIESIFSKTVSL
                     NAFMKSTTAINILVNKTTAFTKIANNSTAFNAMLTISENNVTIANNTTAMGIIANNAQ
                     AMSTVANNDTSISVFVNNTTAMGIIANSSTAMTKITLTGLALNRMVKSNTAKSILISK
                     NSTLQTYKNNIQNTIQGSTAYFRTITGFADADDNPPQTINSTYVGITYCYGYKGNSYY
                     GIVYHGYNTSIEAGRGNGYKDETKKFITLGGARYDQSGDGYFTYAMYQAI"
     gene            3375..3638
                     /locus_tag="KPG09_000846"
     CDS             3375..3638
                     /locus_tag="KPG09_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965471.1"
                     /translation="MEELITQLSGLGAVGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVEMGAMKTDVEEIKEDVTDIKDMLQKEGL"
     gene            3639..4445
                     /locus_tag="KPG09_000847"
     CDS             3639..4445
                     /locus_tag="KPG09_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0965472.1"
                     /translation="MRVALTAGHTLTGKGTGATGYINEGTENRILMDLVVKWLKKGGA
                     TVYSGKVDKSNNYLAEQCQIANKQNVDVAVQIHFNADHTTLNVMGTETIYKTNNGKVY
                     AERVNEKLATIFKNRGAKSDARGLYWLSHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKTIDNKENSEDKKMYKHTIVYDGEVDKIPATVVGWGYNDGKILICDIK
                     DYVPGQTQNLYVVGGGACEKIGSMTKEKFTMIKGNDRFDTLYKALDFIDR"
     gene            complement(4493..4705)
                     /locus_tag="KPG09_000848"
     CDS             complement(4493..4705)
                     /locus_tag="KPG09_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965473.1"
                     /translation="MTWKNEIKSILVKENISMNELNNLMNEYNNKNNTVENLRQKINN
                     ETMKYSEILTIANLIGYEVVWKKKEK"
     gene            4884..5714
                     /locus_tag="KPG09_000849"
     CDS             4884..5714
                     /locus_tag="KPG09_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965474.1"
                     /translation="MKTLFKEAHKIAREIKEKYNDVDYKVQFSLCLSFLNKKGDIKMK
                     ELKGSEKQIIWANDIRTDILNLTNELEKSKIERIKNEDYKIKDMSIEEMTERCKRKFE
                     RIREAISNIEDAKFFIDNFRNVLKYNSLNQKAFQINQIFRESQFTEEIGNLKFLKGET
                     QASHKLRKVEGSISYEEAKKIAKEINFNDNWSNEIKENVLEIVEIFDDAVKQATSTEY
                     IEKAKKYIIETLIQKNAQFYKNGFADIKKKQIENYRKIESAERKIEIAYQIFRDGVEC
                     "
     gene            5793..6299
                     /locus_tag="KPG09_000850"
     CDS             5793..6299
                     /locus_tag="KPG09_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965475.1"
                     /translation="MNIDFSTILNLKVNKENVEMIEKEINKINNDIFDISLKDCANSH
                     VGIVKETVGKSLNIETWGHGEINSSIGIRLRFNFYYSKGDKVDIENIENILIYVNSLC
                     SKNVESDLIASVRPGFYKLEEKYSTRVEKCEIRHEFTGINKQEIKEFLEYLTEIEKSE
                     RTVMYKNN"
     gene            6319..6789
                     /locus_tag="KPG09_000851"
     CDS             6319..6789
                     /locus_tag="KPG09_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965476.1"
                     /translation="MNKQKARRFLRVIDMNIDKIEEEAIKAFKESCLIKETNNIKIYI
                     DIQGKVEAIAVQTWAKLLGDDKEINIFTLNQAPTHLNDMLGEICYVNDYEEFENWCEN
                     EWENLDWDSYKKFNKENFEEIAERNIDDSTSVFLEELQKGIESCKQELQNVIEN"
     gene            complement(7022..7303)
                     /locus_tag="KPG09_000852"
     CDS             complement(7022..7303)
                     /locus_tag="KPG09_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965477.1"
                     /translation="MNVYLQIGKELSEKMTKSELNKFSQEVTNYLRFAGHDNDMKIKL
                     FQTIIEAIARKRVTLPADNEFTQGIMLSNELEMTTNVGFIIEGLSYHNN"
     gene            complement(7447..7779)
                     /locus_tag="KPG09_000853"
     CDS             complement(7447..7779)
                     /locus_tag="KPG09_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016040102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965478.1"
                     /translation="MPSFNRDLLVDKEKPILKNGTIVQNDSTTTDNKTLNIYNLVNKK
                     SKKVTMSATLDEELVNKLKSFSIDMNSDVSKLLSDILTQILSDVTIKEENLNIYNERN
                     RRNKTKKK"
     gene            complement(7780..8589)
                     /locus_tag="KPG09_000854"
     CDS             complement(7780..8589)
                     /locus_tag="KPG09_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBG0965479.1"
                     /translation="MANTKLLTYFNIKGGIYKTTTSIMTAYELAKDKDKKILLWDLDV
                     QANLTQYVYEINHNDNTTLDILKGISANDAIVKSPNENYINVDLIPSDIQMARFEQEL
                     SPLPAREKFLARWYMQNFNTLSEYDYIICDLSPRYDLTAKNVLFLADSIIIPIQDKNI
                     SSLRGAELFKQLWDVDRTYFDKEDNIKSTILVGFEKKKTQISDTFDSYLEGFNDMRDI
                     MLDTYIRKNEFIEKALLKKLSLTDYTKITKEHFSRQEFTNMLEELKRKGVL"
     gene            complement(8878..9060)
                     /locus_tag="KPG09_000855"
     CDS             complement(8878..9060)
                     /locus_tag="KPG09_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBG0965480.1"
                     /translation="MEKEKRDLKFNFHKGGSGSFTPRMSVPKKWADEMGITRENPNVT
                     ATFEGDRIIIEKAKNE"
     gene            complement(9881..10033)
                     /locus_tag="KPG09_000856"
     CDS             complement(9881..10033)
                     /locus_tag="KPG09_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965481.1"
                     /translation="MDNFLLNVLAGVIASLIFYIISKLFRQVKNHSAQKSGWEFDLRI
                     KFKKFK"
     gene            10553..12718
                     /locus_tag="KPG09_000857"
     CDS             10553..12718
                     /locus_tag="KPG09_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG0965482.1"
                     /translation="MKNVNKNNIDKMFLEECSAERAEHEKENYSNFNRHETYEIKKYI
                     NEKKLGIKTAIANAINGGKTLFIAPTGAGKSYSFINTLKKLKTKALFILPNASNVEQA
                     MHEYDIPGAYDKIPVSEAFKNGNLVVMTWDKTEKLINTDLSEYIIVVDEIHQTYTDAY
                     RGKAIKNLNNIMSKCKGRIDITATPTKLEFEIYDYIIEYTQKQKTEYKVKLYNDFDNK
                     NFTEMINIINKSKNSAMLMNDISTLEFIRDSVNKKVGVVYADGKEENELYNRIVKNSD
                     MKDYETLLNTTTILAGVNINNKDITDIIIVNIKDVGAIKQYVARFRNLKKVNVHIFNK
                     YEEECNVYKIEWLVNKNIEKATILKDAYNKVSKHMLQFETVGINATPIRIDSNVYYCT
                     KDNCYKVDKLYIKSQVYSNYYNTRTIQSFKVLLEEYFENVNITDAKEVGTNEKELKEY
                     KKVVKEIKDATREILKEHKEILVGYRQIKSDSKSFSLMQYHNDMKLSSKGCLEAYRAY
                     DIHNLVKKSKSNSMLELYSNYVLDNKFDLDLAWKLANTANKRRGVIFNKINTLIYREL
                     KEEYPEFLNDELIQVTVFNYIDKLFGIGTSYKELHLQELSNDLRTILGENWDLTTKKL
                     SVTLNGIFIIEKKKYSNWHDIETMFFYKNINPISCQFEKKRIQLNNIKRYITVDDIKK
                     DLELDEKDKSLENAIKYTKDRMLNSLDEDEKLLLLKGFM"
     gene            12765..13112
                     /locus_tag="KPG09_000858"
     CDS             12765..13112
                     /locus_tag="KPG09_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965483.1"
                     /translation="MFELDIKVKNKYIVSVSYEFSELLIRAFNDYDNFIKDIKNKDKF
                     IPIGINLGYRELLEENSAYHEYNFYYDEEVDKYFFELQYQEQDLREELSKQLLLELIK
                     INLPAGAELIVVD"
     gene            complement(13928..14281)
                     /locus_tag="KPG09_000859"
     CDS             complement(13928..14281)
                     /locus_tag="KPG09_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0965484.1"
                     /translation="MNTRIRDLRIAEGLSQIEFGNKLGMTRHEIYNLESGRTRIKESD
                     LKLITSTFKVNEKWLKTGEGDMYTFSNNNLITAEVLCAIDKNERLAKAMLKFSKLNDK
                     ELEAMEKLLELFQKE"
     gene            14454..14636
                     /locus_tag="KPG09_000860"
     CDS             14454..14636
                     /locus_tag="KPG09_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965485.1"
                     /translation="MTREERILRNAILYLDMSEEKQRIVDCILNAKTIENIVENDILD
                     VNKLNEAVKEIEQIIC"
     gene            14703..15128
                     /locus_tag="KPG09_000861"
     CDS             14703..15128
                     /locus_tag="KPG09_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965486.1"
                     /translation="MIKVILCSTIDEYIEYEDVIGAKDSYLMDVIIGGECSEIDAIET
                     LEILKNSKARIVSEAEKTCLKFGIGNQISKAIEQGIKEGSKLKRRIHCDNCGAIVDSS
                     EELTSMPVLENYNNNQSVSDQLGSIDVCPNCLLEITDLL"
     gene            15203..15607
                     /locus_tag="KPG09_000862"
     CDS             15203..15607
                     /locus_tag="KPG09_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBG0965487.1"
                     /translation="MINLETLCNGETKEKIENGFMEIFKNIQDPNTPATSTRSLTVKV
                     TLKPGKNRSHVNTQIQVIPKLASVLPSETDIIVEKDFRTGEVNANEYGNQLPGQVKLG
                     DLESKETSVTEETEVKESEEDNVRKFKSLKDL"
     gene            15630..16343
                     /locus_tag="KPG09_000863"
     CDS             15630..16343
                     /locus_tag="KPG09_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965488.1"
                     /translation="MLTDLFNRVMEVGARKIETREINGVTYTTDPLIEVHPRPAEIVR
                     LSTLDGLIGYIKSSIDSKLKSGKLMVQVESPKSVILCSELLESGKRDIYIESRALLPH
                     VNFDCSLDAEQFNVMLQSSFVDTEDKKLLLKVSGNVKETSVKTVQDDGVSQVVTMSTG
                     VASVEDVILPNRVKLKPYRTFAEVDQPESEFIFRVKEGIAFKLIEADGGAWRLEAIKN
                     IKEYLEKKLEGIKNIDIIA"
     gene            16399..16563
                     /locus_tag="KPG09_000864"
     CDS             16399..16563
                     /locus_tag="KPG09_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965489.1"
                     /translation="MYKDITKMGAIERGEYVGNVALELKEENPHLSKIDLIELALKKI
                     REEKEQEAGQ"
     gene            16560..16871
                     /locus_tag="KPG09_000865"
     CDS             16560..16871
                     /locus_tag="KPG09_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965490.1"
                     /translation="MKIGKYLIVSQEQVDRNIECLKEENKVRDDKIRYYERIMSNNEE
                     LIQILEEQLKEYKSELDVYSLFIDCILNKETRKAIAIYNRTKSIRIKQKCLNSIPIKK
                     G"
     gene            16917..17066
                     /locus_tag="KPG09_000866"
     CDS             16917..17066
                     /locus_tag="KPG09_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965491.1"
                     /translation="MSEFVQCCCCERTINIEENNHVQYEKEALGLVFTLYFCLNCVDE
                     LSEME"
     gene            17097..17270
                     /locus_tag="KPG09_000867"
     CDS             17097..17270
                     /locus_tag="KPG09_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3797 domain-containing protein"
                     /protein_id="HBG0965492.1"
                     /translation="MKMKNVLKLSAMYCFCPKCGSDELGEGEGSLIVDEYTFHRKCKC
                     GFDIIVDERNDEI"
     gene            17383..17788
                     /gene="ssb"
                     /locus_tag="KPG09_000868"
                     /pseudo
     CDS             17383..17788
                     /gene="ssb"
                     /locus_tag="KPG09_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            17801..18166
                     /locus_tag="KPG09_000869"
     CDS             17801..18166
                     /locus_tag="KPG09_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965493.1"
                     /translation="MIKKVEKIFSGSVNLNFMEDDLTFKNYLALYCCIKPVKVHGNKK
                     VLASSTKACSALKIREHQKKERKKVRLVNAKTGEIKEMAIDEAENFLNVKDLYPVIRR
                     GRPTRTGWYVKDVSNEERY"
     gene            18150..18323
                     /locus_tag="KPG09_000870"
     CDS             18150..18323
                     /locus_tag="KPG09_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965494.1"
                     /translation="MKKDIKYNVIYFNSRTLRMDKESFSTLIDARVCKNEKIKEYENV
                     EIIKKTIIEKLMV"
     gene            18335..18907
                     /locus_tag="KPG09_000871"
     CDS             18335..18907
                     /locus_tag="KPG09_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0965495.1"
                     /translation="MAKKKRICSWCGKSFFTESKNGFCCKSCERKYKKDKEKNVSSCL
                     VEETKEYLIELGNKKRRVDILRKEIDFLKDKEFYREINFNELGFKIHSSPKGIDEMIM
                     NNEDKISFKESEIDFIEYRLRLMDIYISELTEEKQQIIKFMYFYDGASKLSNEEIASK
                     IKCSRSNVHYKHKEALKELAEMLFESGSLL"
     gene            19085..19693
                     /locus_tag="KPG09_000872"
     CDS             19085..19693
                     /locus_tag="KPG09_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0965496.1"
                     /translation="MGSKKPANPIKDKRMVLNIQEYLKEKSVRNYVLFVLGVATGYRA
                     GDLVKLQVRDVRNAIDEGYFLIMESKKEKTKNIRKKNKKPRKAPIVPNLERVLKSYIR
                     DKKDYEYMFPSRQKSVTPHIGVERVTVILKEAGRYFGLKHITAHSMRKTYARTIYEES
                     GFDIIRVKEMLGHSSIEETKAYLGLNEEQYQEYSMFLNDLIG"
     gene            20159..20728
                     /locus_tag="KPG09_000873"
     CDS             20159..20728
                     /locus_tag="KPG09_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG0965497.1"
                     /translation="MIFGYCRVSSKVQIDNNSLEQQEQEIKRHYSDAKIFREQHTGST
                     THRPIFEEMVNQMRDGDKLVVTKLDRLARNATEGINLIQNLFDSGISVHVLNVGLLEN
                     TTLGKFFITTLLAVAEMERNLILERTFAGKEIAKQNPNFREGRPKKYTKMQMEHALEL
                     LKTNSYTQVEKMTGISKSTLQRAKRKQED"
     gene            20757..21464
                     /locus_tag="KPG09_000874"
     CDS             20757..21464
                     /locus_tag="KPG09_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965498.1"
                     /translation="MICISIKNSNREKAFEIYKEKNGRIKLIDISKMLEEKSCNISRW
                     KKIDRWDYRLGINKKVGAPAGNQNALGHEGGAPVNNQNARTHGFFSKLLPARTYEIVK
                     YIEKDGGNSLDILWNSIVVQYAKILDSFKITHVKNKKDHTTDIKKNGNKIKEYEIQHS
                     WDKVTNAMKAEATAFKALSKMIKDYEELLHKNWDLATEEQKNRIENIKARTNKLTGND
                     LEIEDIEEIEAEIYGSN"
     gene            21451..22773
                     /locus_tag="KPG09_000875"
     CDS             21451..22773
                     /locus_tag="KPG09_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBG0965499.1"
                     /translation="MAVTKKKTIPFLFGDKHKEYIKKCAINTYNIAEGAVRAGKTVDN
                     VFAFAHEIKTSKDKIHLATGSTSANAKLNIGDANGFGLEYIFRGQCHWGKFKGNECLY
                     IKGISTKHKQKIVIFAGGAKADSYKKIRGNSYGMWIATEINLHHDNTIKEAFNRIIAS
                     TNRKIFWDLNPDNPNSPIYKEYIDSYMKKDREGTLLGGYNYQHFTIHDNITVSDERKR
                     AIISQYDEGSIWYKRDILGQRCVAEGLIYEYFANNKEQFKTETIDPLMDVIVGVDFGG
                     NKSYHAFVATGITYNYKKVIALASERPSADTSPDELNALLINFIKKVINLHGKVDCIY
                     CDSAEQVLIKGIKNAVEKENLNVSVRNALKNAINDRIRLTDLLISQGRFEYTRYSETL
                     VSALCGAVWDSKETNEDVRLDDGSSDIDSLDAFEYTIERYLKKFIRGD"
     gene            22774..24186
                     /locus_tag="KPG09_000876"
     CDS             22774..24186
                     /locus_tag="KPG09_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0965500.1"
                     /translation="MFEGLKNAIRGVINKLFNKSSIQNELKVDIAVSDKMSRAIDLWM
                     AMYKNKAPWLNEVTKSLNLSATISSEVARLVTLELESEVVGNDFLNEQYQDVLKDIRK
                     YCEYACGAGGLVFKPYVSGNKIEVDYVQASNFFPTEYSSTGDITSAIFSEIKVKGDIK
                     YTRLEYHNWTSTSYTISNYAYKSNTAKLISYSDDLGKGIKLSDVPEWAELSEELVLEG
                     IERPLFAYFKIPQANSLENSSPLGVSVFSRAVDLIKEADKQYSRILWEYEATEIAIDA
                     DSSMFRRNANGEYEVPHGKKRLYRMLEMEDGDNKWNVFSPAIRDSSLFAGLNQLLRKI
                     EFNCGLSYGIISDAQETEKTATEIKTSKQRLYSTVKDIQKSLEDALEGLIYSIDKWSI
                     LAGFTPSLKYETTFNWDDSVIIDKDSELLAMQQDVASGLIRPELYIMKKYGVTEDEAL
                     KMMPDTRELVEDNPFDKGGM"
     gene            24187..25419
                     /locus_tag="KPG09_000877"
     CDS             24187..25419
                     /locus_tag="KPG09_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor capsid protein"
                     /protein_id="HBG0965501.1"
                     /translation="MLTSNELQEIPKYFVSLFQTLEDFVIADIARRIAKAGNITDMAE
                     WQLIRAEEIGMADKVIKMKIAEILDISFEAVDKLFEENAVKSIESDSALYEHAKLTPL
                     HLNSSEELKNYVSSAKEQTKGKLKNMTGTLGFCTIKKGKVISKKLTDIYIESLDLAQF
                     QVSTGVLDYKTAVKKAVKRLADSGLRFIDYETGWTNRIDVATRRAVLTGVNQMSQNIN
                     NKVINDLDTDIVEVTAHSGARCEGAGIKNHKKWQGKWYSLSGKSTKYPSLKVVTGWGQ
                     GDGLGGWNCSHQFHAVIPGISVPAYSKEQLKNIDPPDIGYKGRTYTHYQALQYQRKIE
                     TSMRQTKRQLIAYESAGLKDEFTNASIKLQRQKQEYREFSKIAKMRLQNERHQVFGYN
                     KSVSQKAVHASKKERKNG"
     gene            25412..25696
                     /locus_tag="KPG09_000878"
     CDS             25412..25696
                     /locus_tag="KPG09_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965502.1"
                     /translation="MGKYRKKAIIVEAFKWLGSVEQKEEPTWIVEAIKSGNVWIEQNL
                     GELSPRMYIKTLEGIHEANVGDYIVQGIKGELYPCRADIFRETYDEVLYR"
     gene            25826..26398
                     /locus_tag="KPG09_000879"
     CDS             25826..26398
                     /locus_tag="KPG09_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBG0965503.1"
                     /translation="MKREFLKDLNLEDEVIEKIMSENGRDIEKYKKEVEKKKEELESK
                     NTELETANNKIKDLEKIDVESIKKEVDDWKSKAEQAQKDKELIENQMSEQTYNLNLDN
                     YLSNFKFSSNLSKEAVKIKMKEKGLEYKDGAFEGADDYIKELQANDPGAFISGNSVPK
                     IVSSSGGDAGESKVSLMEQMIAKNREMLNI"
     gene            26409..27482
                     /locus_tag="KPG09_000880"
     CDS             26409..27482
                     /locus_tag="KPG09_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage coat protein"
                     /protein_id="HBG0965504.1"
                     /translation="MSLFDSKIFNGEVFGKYVETVPNLNRDELIRSGAIRRRDDLKAM
                     FSAQSGANYATIPMLGLIDGDPVNYDGQTDIVATTTKTFSQSVIVVGRAKSWVEKDFS
                     QDVTGGVPFMENVGNQVAKYWDNIDQKTLLAVLEGIFAMTGAKNLEFVNNHTFDITKE
                     AETENQKVKAETLNSATQKACGDNKEKFALVIMHSVISTNLENLNLVARLKYTDPQGI
                     QREMKLGTWGGKLVLIDDNMPTREVAESSEGSNDGYTEYTTYVLGENSIDFEDVGAKV
                     PYEMDRAPGKNGGEDYLYSRQRKVFAPYGISFTKKAVATASPTDSELKNGANWELVND
                     SSDGSKTYIDHKAIPIARIISRG"
     gene            27486..27818
                     /locus_tag="KPG09_000881"
     CDS             27486..27818
                     /locus_tag="KPG09_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965505.1"
                     /translation="MNMYIDYEFYSALGGEIPQDEFNKYAIRATKYIDYNTFNRIEEV
                     TEEIKIATCEIADLIYKSNLEGNKEVQSESIGSHSVTYSTNNKTIEQKAYDILKMYLD
                     SDLLYRGV"
     gene            27820..28254
                     /locus_tag="KPG09_000882"
     CDS             27820..28254
                     /locus_tag="KPG09_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965506.1"
                     /translation="MFFKDNITLYNKYYDKSSDKTLYKRTYLFEVDYQEAKNIAVTDK
                     GLLSADSVKVVVPFSVNARGKKYIDPFKYYELEENEKNKFYTFKVGDIIVKEVVDFEI
                     TSVKPYTLRELQSKFDDVSIIKSVIKCDFGSIRMRHFELEAE"
     gene            28261..28608
                     /locus_tag="KPG09_000883"
     CDS             28261..28608
                     /locus_tag="KPG09_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBG0965507.1"
                     /translation="MNVHVDINATRIMRAKGLGRGGKAQKFLTHEVRRLSDSYVPKQS
                     GTLKNTAREEVDKIIYIQPYAKPQYYNNAGCGSEGIGAGGKRGKHWEKRMYADKGDEL
                     IGSVAKFVGGRRE"
     gene            28605..29036
                     /locus_tag="KPG09_000884"
     CDS             28605..29036
                     /locus_tag="KPG09_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol resistance protein"
                     /protein_id="HBG0965508.1"
                     /translation="MTLVESVREFIKKCPYLDEFAKSINVEFLGEEFTSYTLETVPSE
                     TVIKRFINGDSIKQFIFIFASRECYGSDVMQNIENSQFYEQFADWIYRENLSGNLPVL
                     SGDKEAMSLEVSTPGYPVQTGIDTAQYQIQLKLKYFEKGEM"
     gene            29039..29839
                     /locus_tag="KPG09_000885"
     CDS             29039..29839
                     /locus_tag="KPG09_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965509.1"
                     /translation="MAGMETIQRYRVADYLGVLGDDGQNSWVLMGAGFNTLDENPSAQ
                     SDSKTYINDRNSTSAIKSYQTQFSFETDLIKNEEAIMELYKIGRDQATGADAERDYLR
                     VELFLPIKGKENTFRARKFRVSVEVSGFAGAGGETITVSGNLNTVGDLVHGEFNTQTR
                     TFVEDGAEAPPAESLKVLTINSVAGTNTGNTKITVTPTLESGNSYKYKTGANITVPTL
                     DTDCSVGYTAWDGVEEITATKGNKILIVEVNANNKAKKAGITTITVKE"
     gene            29855..30217
                     /locus_tag="KPG09_000886"
     CDS             29855..30217
                     /locus_tag="KPG09_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965510.1"
                     /translation="MIINNIELEDLEIYDADVMERVETSIKTAIDGIQIAETEEKENH
                     AIFRRICDLIFACFDNIFGDNTSNKIFAGKRNVKVCMIAFSELADEVERQKKEGMELF
                     SSIGNKYSPNRTQRRAKK"
     gene            30217..30822
                     /locus_tag="KPG09_000887"
     CDS             30217..30822
                     /locus_tag="KPG09_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage Gp15 family protein"
                     /protein_id="HBG0965511.1"
                     /translation="MNMLVDILPESVEIDGKEYKINTDFRISILFEMLIQDNSISDEE
                     KGENALLLYYPVIPTNTTMAIEKIIWFYSCGKKENNVDDGCRGGAGSSKSQIYSYDYD
                     DEYIYSAFLGQYGIDLQDIEKLHWWKFKALFKSLKEDTEIVKIMGYRAMDVSGDMPKA
                     QKDFYRKMKKIHAIPLPQNEVEKINEIEKALLNGGDISNLL"
     gene            30895..31536
                     /locus_tag="KPG09_000888"
     CDS             30895..31536
                     /locus_tag="KPG09_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBG0965512.1"
                     /translation="MGFKFRKSINLGGGLKLNINKNSVSVSGGVKGARVSVNSKGKAT
                     TTLGVPGSGLYYQEVANLGGNKKRNSKSVGSSESSRQAQQLLKIINDCANIVNTTKDP
                     KTFFYRYNMLLDKSYALVAIEDDLNFSGQSPSQMLDSIIAKRTDTINDFLTRYYNEMS
                     SKLKELKTEKARMNNAYKFSSLAIDYKEHLNNDNKAKLNRLYKQLLDDVKRQD"
     gene            31647..36797
                     /locus_tag="KPG09_000889"
     CDS             31647..36797
                     /locus_tag="KPG09_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBG0965513.1"
                     /translation="MADGSIIIDTLLNTEDAEKQLNNLADTMKKKAKSALAVAGIGAS
                     LGAIGKQAIDFGDEYQKAMNSFESATGNAEAKAKGFGEALQQVYANNFGEDMGDISEV
                     MSLISQKLDGIDASNIQEVTESAIMMRDTFDMDIGESLNGVNSMMRQFGVSAKESYNL
                     IAQGAQQGLNQNDDLGDQLAEYSTYYAQMGFSAEEMFNMMKNGAESGVYQIDYLNDAL
                     KEFNIRTKDGSNGTKEAFEALGFNADELTKKFANGGSSAKEAFIQVTTALNNLDDNVL
                     KNQIGVQLFGTKFEDLEADAVTALTNIEGSISSSKDKLEEINKIKYNSFGEAVTGIGR
                     QMQVNLLLPIAEGLLPVLNDLANKFAKAFQNENTKNAIRSTAESIGGLIGTVVKLISL
                     ALTPLINVITFLVQHGSLVAPLIAGIGVAFTSLKVAGIVRGIQKSFTDAQLAVRIFTS
                     GVTESETKLTLYQMVVGLVTKKISLAQVATNLWKGALTALGGPVGLTIVAVTALTAVV
                     VSLWKNNEAFRDAVIKIWNNIKDAGAKIFGQIAKFFTETIPQALSKAISFIKDNWQGL
                     LLMLVNPFVGAFKLIYDNCESFRNKVNSIFNKIIGIFKNVLNFIKNNWKNIGSMLLSP
                     FVNAFKAIYNSCVNFKNKVFSFFSNVVKGFATFGKNIIVGIINGLSSGIGLVVDTVKN
                     IGLIVVNTFKKVLRIHSPSKVTTELGEFAGIGFANGIKNTSKDVVEATKELISAMEKN
                     LDTNKSKFEKICEAITTALKNQYESQKDIQIKALDERLKVEEKASNDRLKVYEKEYNE
                     KLKYLDTETNEKTNAIQEQIDAIDKQIEEEQKAEEEKAYNEKISDLDRKLATAKNQKE
                     REKIQKEISEAQSDRQKKLLDEQRQQEKNRLKEQIENIKTEASNKKEQYKEEYDNQKE
                     AESKKLELIKESNASQKEEIEKYFSELLEETNIQNEARRLLLQKNSEEIIKLLSEYNP
                     HWQDAGQSLADSLLNGVNSKKQSIQEAVKEAINLKEIIPAQEKELDRLKKKLEEYEKL
                     KEKANTSSASGGDVDSSAKSSSLDSGSAKLDAGELEEYADGIDDVKTSVEGLELVSDE
                     LVTGTVPKVGDSASKLSESIKKGFNGIGGFFGGIEKWLRDTDKNINEWLASAKNSIGD
                     FFSKVKDKFKEGIENIGGFFGGLGNKISEGFAGAGDFFTEFGDKAQQGFSSIKDKAVE
                     IFGGLSNWFKETGSKIGDAIGSGLKSGLDFFTSTVPKWFAGIGDKIKEECGKIGEKIS
                     SFFNETMPAIINNIVEWFKQIPYNIGFVIGSIAGFFVDLGANLYAWATETLPEIINSI
                     VEWFVSLPEKISEFFSIILQNIQTWGENFKVSVSEFFGAIWENITQFFTELPERISEW
                     FNTVVETISGWGENLKQLATDIFNQFVDNVLIPISELPGKLWEKFTDIMGKVSEWGSN
                     LIQKALETGSKFLENIMKFFSELPGKIWEKLTNIMQKVMEWEVNLSKKALETAKNFFN
                     NIYNTVSQLPGKFREWLDNIISNVISWGTNLVKQAGEAGKNMANAVKDALKDLPSKIM
                     SIGKDVVRGLWEGITGMGGWLKDQVLDFASNVIDGFRDGFGVHSPSIIMRDLIGRNLV
                     KGVGVGIDVETPGLKEKIEKNISELTGKLKATVNFETSKIQANIVASTDFKAGKETAI
                     MSNNKADEANENQAGIKLNIENFVNNRQQDIENLFDEILFLAKRKGAL"
     gene            36918..37652
                     /locus_tag="KPG09_000890"
     CDS             36918..37652
                     /locus_tag="KPG09_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HBG0965514.1"
                     /translation="MFFVYNGRDSREFGLKIYNINDLSAPQMEVERVSVPGKDGDLLL
                     KKGFENFTLTIECDIDARQSNIEEVATEIKKWLQGDISYKKLFLSNSDFYYLASCNNK
                     LDITRNFKNFASCLLTFDCYPFRYAEEEIISLNVLNLKSATITNFYRESKPVLYIEGA
                     GDISIKINTQSIVLRGVAENGILSDLIIDSEQMNVYRINKENNIIVNENNKLFSDFPI
                     LEEGENQISWEGDIKSIKINPRWNIL"
     gene            37769..39781
                     /locus_tag="KPG09_000891"
     CDS             37769..39781
                     /locus_tag="KPG09_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG0965515.1"
                     /translation="MNDHVREEESGVFELEFTIYEDSFLFKEIKVDRLIKADTSPDYK
                     DQLFRIYYISKNLGGYINVKAQHIKYDLLNNFIESLELNDITCEEALERVFRACEEQN
                     RFRGHSDIKARNTINIEMQSPYSAICEGENSLIQKFEPTAKLFFDNFNVFLNYQRGES
                     KNVLLAYKKNITGLEAEYDTQDIVTKIYPYAVYEDEMITLTEKYIVSPNMNKYATQKI
                     VAIDFSSDEVSVEEELREKCKDYFKYNQVDLPKVLYKVNFVDLSTTINYKDYKMLETV
                     NLGDEVIIRDFNLNINATARVVKTDYSPINRKYYSIEVGDLINHLDFLNNKFNNIENK
                     IETVKKAIDNVKVDDSEFPDTLPNTPALTAEGLFAFINLTWTFTNKSYYKYEVYASQI
                     KDFVPDTINFTNRIFVGQASAYVHQAEPFQTWYFRVRAVNSHGNVTEFSNQVEAQTTK
                     IDDGTMWIEKGAIADALIGELKLDRGWFGQLKGMYINAREMVVVNDNGVKTMEIDSFG
                     DVHFNPNTFKIAFNSVSPYVLIDDEGLKLISGKGFTKMTDSGLYTKFYTGSDVTESYY
                     LQESGYTYFDYKDSKIIIDLPKKFAGKNFKATCAIRSTSTNGSCLSYFSTSATAKKSD
                     TNPQLELSASVRGVSYEMSGSQTIGYWLGMNFFSEGFITVQYYVYA"
     gene            39808..40050
                     /locus_tag="KPG09_000892"
     CDS             39808..40050
                     /locus_tag="KPG09_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965516.1"
                     /translation="MVTIFYSKRTGEIYNCIKSEMEQDYTCFADREEDYRQILDKIVV
                     EDIPEILNPRDYKIENGKFVLKEKEIIKEFTPKMVE"
     gene            40065..>40531
                     /locus_tag="KPG09_000893"
     CDS             40065..>40531
                     /locus_tag="KPG09_000893"
                     /inference="COORDINATES: protein motif:HMM:NF022117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HBG0965517.1"
                     /translation="MRDRIYTVDINTKSYQVAKYKQYDNAIEFKINLLENNIEKDLTG
                     YTAIANFQRPDGKIVYQSCTIENSIATTIIENNITEVAGDVIVEFTFYKDDLVVTTFS
                     LKINIEKSIDKNSITEEPKWDYISVTINQVKEVVEGIEEIKETEEARKEAEIK"
BASE COUNT        16102 a         4767 c         7738 g        11924 t
ORIGIN      
        1 caacattaat agacgaagtt aacacagtta aagcggaagt tacaacagct aaaaatacta
       61 tgattagcga agtaactaca gctaaagaaa ctatgcagac agaagttaca gacgctatta
      121 atgcaatccc cacaaaagaa gagttaaaag gggtaggaat agaaataaaa ggaagtttag
      181 ataatatatc agcacttcca gtcaatccaa ctttaagtga tgcttacttt gtaaaaagcg
      241 caacaataga aaaccaaatt gatttatatg tttgggacaa cactaattgg gtcaaagttc
      301 ctgacataaa aataaagggt gaaaatggag atggcttaga atttaattgg gatggcacgc
      361 gactaggtat aaggatagaa ggacaggaga attatactta tacagattta aaaggtcaaa
      421 aaggcgataa aggtgatagt attgaattta attggaatgg cacacgatta ggtataaaga
      481 tagaaggtca agaaaattat agttatacag aattgaaagg ggagcagggt tatactccaa
      541 caataggaga aaatggaaac tggtggatta ataatataga tacccaaaaa ccagctagag
      601 gggcatcttt gaggatttta ggaaaattag attctataga taacttacca ttagacccta
      661 ctatcggaga ttgctggatt atagggagaa atatttatat atatcaaact aaatgggaag
      721 acttaggttc tctagcgggc gtggacggca agaacctaga atttaattgg gatggcacac
      781 agttaggcgt taggcaacaa tacgaattag attataagta tatagacctc aaaggtgaca
      841 atatcgaatt tgcatgggat ggcacacgac taggtgtaag gatagaagga caggagaatt
      901 atacttatac agatttgaaa ggtcaaaaag gcgatagcat tgaatttagt tgggatggta
      961 cggagctagg cgttagaata gaaggtcaag aggactatag ctatacaaat ctaaaaggag
     1021 cgacaggaaa taaattagaa tttaattgga atggaagtca gctaggtatt agagaagaag
     1081 gacaaacgga atatatatat acagagctta gaggggaaca aggttatact ccagcaatcg
     1141 gcgaaaacgg taattggttt ataaatggag aagacacggg gaaagcgtca aaaggaaaag
     1201 ttacatggaa tgagttatta gaaaaaccga aagagttaga ttacattaaa aaaagcacga
     1261 cttttaattc agacggctct ataacagata ttttagactc ggttagtaaa actataacta
     1321 aatttaatgc agatgggact atagtagacg aaaaatatat agataatgta ttagtaagta
     1381 aagtaaaaac gacttttaaa ggtaatcaaa tagaagaaat aaaagaagag gtgagttaat
     1441 gagttgggcg gagacataca aagtgaatag tgacttgcaa ggcgagccac taaatttttt
     1501 aagttatttg caagacataa aactaaatgg attagatagt tatgtgctgt ttattggaaa
     1561 tgctaggata tgggaagaat tatatttaaa tagtttatat ttattttctg atagaggaat
     1621 aagagaaaca gtttacacag ctttttcaga aactgatatt gataatttat ttaataaaag
     1681 cactaagcta ggggaacaat taaatgcctt ttatagaaca gatatattta gcttaggaaa
     1741 tgctgataat gtagtaaaag aaatgactat agagcattat aattcattag aggaaaaatt
     1801 taaagcaggg tatgatagat atgttacaag agaacaagaa aagtcaacta taggagcatg
     1861 gtttaattct acttttagct tagataatac tgatttagaa aatcttacaa ctatagaaga
     1921 aatattagca aatgtggaag ctactaatgc aatacttaat aatagtaatg caatagtagc
     1981 tttaactatg tgtaaatcaa gcatggacgc agttgtagca agttcaaatg ctatggattt
     2041 attaggtcaa tatattttaa gagttacaac agagtcacca gtcattagag caattttaaa
     2101 aaataatgtg attagggatg cgattataaa tagtgatgaa gctatgacac aaatttcaag
     2161 taacgaaaac tccgtaatgg aaatatttaa tgacttagaa gctacaaaag tattagtgca
     2221 aaaccaaaat agcataaata aaatattaac taataatgtt actgttgaaa aaataatccc
     2281 taatttatta gaaatgaagt ataatttaca aacttcttta aattatataa atacaataaa
     2341 atcaaatatt gcttctggta aaggtcaaat tatggcaata acttataatg aagaaatatt
     2401 cccgatttta aaaaatgcag taaaaaatta tgatggtatg gaaaccacac gtaatatatc
     2461 acaacgagat attgaggaaa aaataaaaat ttcggatgca attttagaaa gttcaatcgc
     2521 aatggcaact tttgcaaata attctattat agtaaataaa gttggtgacc gagtgggaat
     2581 tatagaaagt attttcagta aaacagtgtc attaaatgct tttatgaaaa gtacaacagc
     2641 tataaatatt ttagtaaata aaactactgc ttttactaag atagcaaata actctactgc
     2701 tttcaatgct atgctcacta tttcagaaaa taacgttact attgcaaata atacaacagc
     2761 aatgggcata attgcaaata acgcacaagc tatgtcgaca gttgcaaata acgatacgtc
     2821 tataagtgtt tttgtaaata atacaacagc aatgggcata attgcaaata gctcaacggc
     2881 tatgaccaaa ataacactta caggacttgc acttaacaga atggttaaaa gtaatacagc
     2941 taaaagcata ttaattagca aaaattcaac tttacaaact tacaaaaata atatacagaa
     3001 cacaatacaa ggaagtactg cttattttag aactatcaca ggctttgcgg atgctgatga
     3061 taacccaccg caaactatta atagtactta tgtaggcata acttattgtt atggatataa
     3121 aggtaatagt tattatggaa ttgtgtatca tggctacaat acaagtatag aagcaggcag
     3181 aggaaatggc tataaagatg aaaccaaaaa gtttataaca ttaggcgggg ctagatatga
     3241 ccaaagcgga gacggttatt ttacttacgc aatgtatcaa gcaatttaat gtttataagt
     3301 catctaaaag attagattaa tagtctaatc tttttttata taaaaaataa ataatcaaaa
     3361 ttaggaggaa agttatggaa gaattaataa cacaactaag tggactagga gcagtaggga
     3421 tattatgcgc tttgctgttc aaaaacacta tgcaggagaa aaaagaagat agagatatgt
     3481 ataaaaaaac agtagaaaat ttcatagaac tatctacaca gcaacaagag ataaataaga
     3541 atatacttgt tgaaatggga gcaatgaaaa cggacgtaga agaaatcaaa gaagatgtaa
     3601 cagacataaa agacatgtta cagaaagaag gtctttaaat gagagtagca ctaacagcag
     3661 ggcatacgct aacaggaaaa ggaacaggag caacagggta tataaatgaa ggaacagaaa
     3721 ataggatatt gatggatttg gttgttaaat ggcttaaaaa gggtggggct actgtgtata
     3781 gtggtaaggt agataagtct aataactact tagcagagca atgtcaaata gccaataagc
     3841 aaaatgtaga tgtagctgta caaatacatt ttaacgctga ccatacgaca ttaaatgtca
     3901 tgggtacaga gacgatatat aaaactaata atggtaaggt atatgccgaa agagtcaacg
     3961 agaaactagc aacaatattt aaaaatagag gcgcaaaatc ggacgcaaga ggtctttact
     4021 ggcttagtca tacaaaagct ccagcgatat taatagaagt gtgtttcgta gatagtaaag
     4081 cagatacaga ctattatatt agacataaag acatagtcgc taagttaata gcagaaggta
     4141 ttttaaataa gacaatagat aataaagaaa atagtgagga taagaaaatg tataaacata
     4201 caatcgttta tgatggagaa gttgacaaaa tccccgcaac tgtagttggc tggggctata
     4261 atgatggtaa aatattaata tgtgatataa aagattatgt accaggtcag acacaaaatc
     4321 tttatgttgt tgggggtgga gcatgtgaaa agattggttc tatgactaaa gaaaaattta
     4381 ctatgataaa gggtaatgat aggtttgata cactttacaa ggcactagat tttattgata
     4441 gatagattag aaggtagcaa ctagagttag ttgttacctt ctttttttat gtctactttt
     4501 ctttcttctt ccaaacaact tcgtatccta tcagattagc aatagtcaag atttcactat
     4561 acttcatagt ctcattattt attttttgtc ttaaattctc tactgtatta ttcttattat
     4621 tatattcatt cattaaatta tttaactcat tcatgctgat attttctttc acaagaatac
     4681 ttttgatttc atttttccat gtcatttttt cacacctctc ttatttatat aataacataa
     4741 aaaaattaaa agtaaaagaa taatctatgt taatcaaagt tatatttttg gaaatcaaag
     4801 aaaaaacttt gaaaaagtgt tgacttagat attcatatag attataatat aattaacaaa
     4861 taaaacaagg gggtaagcaa agaatgaaaa ctttatttaa agaagcacat aaaatagcaa
     4921 gagaaattaa agaaaaatat aatgatgtag attataaagt acaatttagt ctttgcttat
     4981 ccttcttaaa taaaaagggg gatataaaaa tgaaagaatt aaaagggtca gaaaaacaaa
     5041 ttatttgggc taatgatata agaacagaca ttttaaattt aactaacgaa ctagaaaaaa
     5101 gtaaaataga aagaataaaa aatgaagact acaaaatcaa agatatgagt atagaagaaa
     5161 tgacagaaag atgtaaaaga aaatttgaaa gaataagaga agcaataagc aacatagagg
     5221 atgctaagtt ctttatagat aattttagaa atgttttaaa atataattct ttaaatcaaa
     5281 aagcgtttca aataaaccag atttttcgag agtctcaatt tacagaagaa attggaaatt
     5341 taaaattttt aaaaggagaa actcaagcgt ctcataaact tagaaaagtt gaaggtagta
     5401 taagctatga agaagctaaa aaaatagcta aagaaataaa ttttaatgat aattggtcta
     5461 atgaaataaa agaaaatgta cttgaaatag ttgaaatatt tgacgacgca gtaaaacaag
     5521 ctactagcac agaatatata gagaaagcta aaaaatacat aatagaaaca ttaatacaaa
     5581 aaaatgcaca attttataaa aatggatttg cagacattaa aaagaagcag attgaaaact
     5641 atagaaaaat agaaagtgct gaaagaaaaa tagaaattgc ataccaaata tttagagatg
     5701 gtgtagagtg ttaaaaattt tggattggta caaatatagt tcaccgtcga atgctataat
     5761 tttaatatat aaaataaatg gaggattgaa gtatgaatat agattttagt acaatattaa
     5821 atttaaaagt taataaagag aatgttgaaa tgatagaaaa agagattaat aaaatcaaca
     5881 atgatatatt tgatataagt cttaaagatt gtgcaaacag tcacgtggga atcgttaaag
     5941 aaacagttgg aaaaagttta aacattgaaa cgtgggggca tggtgaaatt aacagtagca
     6001 taggaataag attgagattt aatttttatt attcaaaagg cgacaaagta gacatagaaa
     6061 atatagaaaa cattttaata tatgtaaata gcttatgcag caaaaatgta gaaagtgacc
     6121 ttatagctag tgtaagacct ggattttata aattagaaga gaaatattct acacgcgttg
     6181 aaaaatgcga aattagacac gaatttacag gcataaacaa acaagaaatt aaagaatttt
     6241 tagagtatct tacagaaatt gaaaagagcg aaagaactgt tatgtataaa aacaactaaa
     6301 aaatggagga tataaaaaat gaataaacaa aaagctagaa gatttttaag agttatagat
     6361 atgaatatag ataaaataga ggaagaagct ataaaagctt ttaaagaaag ttgtttaatc
     6421 aaagagacta ataatataaa aatttatatc gatatacaag gaaaagttga agcgatagca
     6481 gttcaaactt gggctaaact tttaggtgat gacaaagaaa ttaatatttt cacattaaat
     6541 caagcgccaa ctcatttaaa cgatatgctt ggagaaattt gttacgtaaa cgattatgaa
     6601 gaatttgaaa attggtgtga aaatgagtgg gaaaatttgg attgggatag ttataaaaaa
     6661 ttcaataaag aaaatttcga agaaattgct gaaagaaata tagacgatag cacatcagtt
     6721 tttttagaag aattacaaaa aggcattgaa agttgtaaac aagaattgca aaatgtaatt
     6781 gaaaattaag tagaagagta ttattattaa tgtatatagt atcgctttta agattaacta
     6841 cgagggattt aaaatatttt ttaatttatt tagaaataat attgtaataa gaattttaag
     6901 attaactatg agggatttaa atgtcaattt aatttatata gaaataatat aatatccaat
     6961 tttagattaa ctatgcggaa ataaaaacag tgtattcaat aaatacactg ttttttaatg
     7021 tttagttatt atgataactt aatccttcga ttataaatcc tacattcgtt gtcatctcta
     7081 attcgttact taacattatt ccttgtgtaa attcattgtc agctggcaat gttactcttt
     7141 ttctagcaat tgcttctata attgtttgaa atagtttaat tttcatatca ttatcatgtc
     7201 cagcgaatcg tagataattc gttacttctt gcgaaaactt attcaactcc gatttagtca
     7261 ttttttcaga taactctttt ccaatttgca gataaacgtt catcttttcc aacccctttt
     7321 tagattatag ttaaaacata ttatttaaac aatattaata tattgtaata ataatattaa
     7381 aatattactt aaatattata tatacaatat ttaagtaata tctaaacaat ataaatataa
     7441 tattatttac tttttcttag tcttatttct tctatttctc tcattgtata tatttaaatt
     7501 ctcttctttt atagtgacat cagataaaat ttgagttagt atatcactta ataatttact
     7561 aacgtcgctg ttcatatcaa tagaaaaaga ttttaatttg tttacaagct cctcatcaag
     7621 cgttgcactc atagtcactt ttttactttt cttattaact agattatata tgtttaatgt
     7681 cttattgtct gtagttgtag aatcattttg tacgattgtt ccatttttca aaatcggttt
     7741 ttctttatca actaaaaggt ctctgttgaa acttggcatc tataacactc cttttctttt
     7801 caattcttct aacatattag taaattcctg tctgctgaaa tgctctttag ttatttttgt
     7861 ataatctgtt agagatagtt tttttaatag tgccttctct ataaactcat tttttctaat
     7921 gtatgtatct aacataatat ctctcatatc gttaaaccct tccagataag aatcgaaagt
     7981 atcgctaatt tgagttttct tcttctcaaa tccaactaaa atagtacttt ttatattatc
     8041 ctccttatca aaataggttc tatcaacatc ccagagttgt ttaaataatt ctgctcctct
     8101 taaagaagaa atgtttttat cttgaattgg aataattata ctatctgcta agaaaagcac
     8161 atttttagct gtcaaatcgt agcgtggaga taaatcacag ataatataat catactcact
     8221 caaagtatta aagttttgca tgtaccagcg agctaaaaat ttttctctgg ctggtaaagg
     8281 tgatagctct tgttcaaatc tagccatttg tatgtcagaa ggaattaagt ctacattaat
     8341 gtaattttca ttaggtgact tgactattgc atcattagca ctaatccctt ttaaaatatc
     8401 taatgtagta ttatcattat gatttatttc ataaacatat tgtgttagat ttgcttgcac
     8461 atctaagtcc cataagagta tttttttatc tttatctttc gcaagttcat acgctgtcat
     8521 tatagacgtt gttgtcttat agatacctcc ttttatgtta aagtacgtaa gtagctttgt
     8581 attagccatt ttctccacct cattattata tttataatat tataataata ttgtagtatt
     8641 gtttttataa tattgcaaca atatattaat attgttaatt acaatattca aataatataa
     8701 atacaatatt tacatattgc ttttacaata ccaatatatt atattaaaaa tactaaaata
     8761 ttgtatatac aatactaaaa taatattttt ataatattac agtaatattt ttatattata
     8821 gtaaaattta aaaaaaagaa ggctaagaaa aaaagcaacc ttcttttata gaaaaagtca
     8881 ttcattctta gccttttcaa ttattatcct gtcgccctcg aatgttgccg taacattagg
     8941 gttttctctt gttatgccca tttcatcagc ccatttttta ggaacactca ttctaggagt
     9001 aaacgaccca ctcccacctt tgtgaaagtt aaactttaaa tctctttttt ctttttccat
     9061 tcattagctc cttttttgct gtattacaaa gaaaagcaat aatacaacac taaaaactaa
     9121 accgagcatg ttgcttgtac tagagtttaa tactatagat actatattta agactatagt
     9181 tatcaaaact aaaacaatgc aaatcttata aaatttatca ctcatgtttt tttagaaatg
     9241 tgttataata ttagaaagaa gtcacctaga gagaagggct attccctcta ggcttatcct
     9301 tacagttctt tcaaaattct tagaactgcg aggattatgt taagtaataa taggatgaag
     9361 gagaaaactt ttttaactat gttaaatttg ttttctcttt tctttttact tctttctaat
     9421 ctccccattc ccgttcctcc tttcttttat ttttttgctt tctcacctcc ttctctataa
     9481 atatattata ccatgacggg tcgtcaaaaa caatagtttt ctcctatttt ttaaacaaat
     9541 tttggtaaaa agtaaagatt ttagtatatt agaaatgatg taaaattgta tcttttttta
     9601 aaaaatgtga tataataaaa gcaaggaaat aatgatttac tttacacaag agtagctatt
     9661 tccaatgttt tgaaaaataa cttattttct taaaccactc ttattggcgt ttgagtggtt
     9721 ttttaatttt atcgttctta aaataaactt tgataaaaat aaaaaaaata gaatactgaa
     9781 aatgatgtaa agttgtatct tttaaagaaa atgtgatata ataaaagcaa gagaactgta
     9841 ttctagtcat aagagtagag ttttagtcta aaataaaaaa ttatttaaac tttttgaatt
     9901 tgattctcaa atcaaattcc caaccactct tttgcgcaga gtggtttttt acttgtctaa
     9961 ataatttgct aattatatag aatattaaac tagctataac accagctaat acattaagta
    10021 aaaaattatc catacttcat cacctccctt tgtagcgttg ggaggataat cttttgtgta
    10081 tagactccac tcttttttta gaatatagat tgctttctct tgctaaaatt attataacat
    10141 atatttacat aaaaaaagac ttagtttata tactaagtct tttaaaatgc agtattttag
    10201 aaaatatatt tttaaaatat aatttatttt caaaaagagt atattttttt ataatttaaa
    10261 tgtgctataa ttataatcac aacgaacaga aaactttttt ttaattggtt taaaaaaatc
    10321 tacaaaaaaa taaacaagga acatgtactt gccgtcaaac aaaccatgtt ccttacaccg
    10381 gaggttagac ctctcttaag gataatatat ccttgttttt ctatacttat atttatatta
    10441 taagacaaag tgtagaaaaa cacaatggtt ttaaggtaat tttaatagta aaattattac
    10501 aaaaaatcaa aaaaagagcg tcagcctcca ggtggaaatt ggagggataa agatgaagaa
    10561 tgttaataaa aataatatag ataagatgtt tttagaagaa tgtagcgctg aaagagcaga
    10621 gcatgaaaaa gaaaattatt caaatttcaa tagacatgaa acatatgaaa taaaaaaata
    10681 tataaacgaa aaaaaactag gaattaaaac ggctatagcc aacgcgataa atggcgggaa
    10741 aaccctgttt attgcaccga caggagcagg aaaaagttat agctttataa atacattaaa
    10801 aaaattaaaa acaaaagcgt tattcatact tccaaatgct tcaaatgtgg agcaggcaat
    10861 gcacgagtat gatataccag gagcatatga caaaatacct gttagcgaag catttaaaaa
    10921 tggtaactta gttgtaatga cttgggataa aactgaaaaa cttataaaca cagacttaag
    10981 tgaatacata attgtagttg atgaaataca tcaaacttat acagatgcat atcgaggaaa
    11041 agcaataaaa aatctaaata atataatgtc taagtgcaaa ggaagaatag acattacagc
    11101 aacaccaaca aaacttgaat ttgaaatata tgactacata atagagtaca ctcaaaagca
    11161 aaaaacagaa tataaagtta aattgtacaa cgactttgac aataaaaatt ttactgaaat
    11221 gattaacata ataaataaat ctaaaaacag cgccatgttg atgaatgata tatcaacatt
    11281 agaatttata agagattcag tgaacaaaaa agttggagtt gtatatgcag atggaaaaga
    11341 agagaatgaa ttgtacaata gaatagttaa aaattcagac atgaaagact atgaaacgct
    11401 tttaaataca acaacaatcc tagctggtgt aaatataaac aacaaagata tcacagatat
    11461 aattattgtt aatataaaag atgttggagc tataaaacag tatgttgcaa ggtttagaaa
    11521 cttaaaaaaa gtaaatgtac acatattcaa taaatatgaa gaagagtgca atgtatacaa
    11581 aattgaatgg ctagttaaca aaaatataga aaaagcaact atcttaaaag atgcttataa
    11641 caaagtatct aaacacatgt tacagtttga aacagttgga ataaatgcaa cgccaattcg
    11701 catagactca aatgtgtact actgtacaaa agataattgc tataaagtag ataaattata
    11761 tataaaaagt caagtttaca gcaactatta caatacaaga acaattcaaa gttttaaagt
    11821 gttattagaa gaatattttg aaaatgttaa tataactgat gctaaagaag ttggcacaaa
    11881 tgaaaaagag ttaaaagagt acaagaaagt tgtaaaagaa attaaagatg caacaagaga
    11941 aattttaaaa gaacataaag aaattctagt aggttataga cagattaaaa gcgattctaa
    12001 gagcttttca ctaatgcagt atcataatga tatgaaacta agttcaaaag ggtgcttaga
    12061 ggcttacaga gcgtatgaca tacataattt ggtaaagaaa agtaaaagta atagcatgtt
    12121 agaactatat tctaattatg tactagataa taagttcgac ttagaccttg cttggaaact
    12181 agcgaataca gcaaacaaaa gacgtggggt aatatttaat aaaataaata ctttaatata
    12241 tagagaatta aaagaggaat atccagagtt tttaaatgat gaacttattc aagttacagt
    12301 atttaattat atagataaat tgtttggaat tggaacatct tataaagaat tgcatttaca
    12361 agagttaagt aacgacttaa gaacaatttt aggggaaaac tgggatttaa caactaaaaa
    12421 gctaagcgtt acactgaatg gaatttttat tatagaaaag aaaaaatata gtaactggca
    12481 tgatatagaa actatgtttt tttataaaaa tataaatcct atatcatgcc agtttgaaaa
    12541 aaaacgaata caacttaata atataaaaag atatataact gtagatgaca taaaaaaaga
    12601 tttagaatta gacgaaaaag ataagagttt agaaaatgct ataaaatata caaaagacag
    12661 gatgttgaac tctttagatg aagatgaaaa actattgtta ttaaaaggtt ttatgtagag
    12721 acaaaaataa atttcaaaaa tatttacata aggagataaa aaggatgttt gaattagata
    12781 ttaaagttaa aaataaatat atagtgagtg tgagttatga gttttcagag cttttaatac
    12841 gagcttttaa tgactatgat aatttcataa aagatataaa aaataaagat aaatttatac
    12901 ctataggcat taatcttgga tacagagaat tgttagaaga aaattcggca tatcatgagt
    12961 ataattttta ttatgatgaa gaagttgata agtacttttt tgaacttcaa tatcaagaac
    13021 aggatttaag ggaggagctt tcaaaacaac ttttactaga acttataaaa attaatttac
    13081 cagctggggc agaattaatt gtagttgatt aaaattgtat ttaatcaaaa agaaacagaa
    13141 tcaaaagaac aattagaaat ttgcatctaa aaacaaaaag tcagtcgacc tggaatatat
    13201 atatatatat atattccagg tcgactgact ttttgataat gagtcatcaa ttaaaataaa
    13261 cataattagc atacaatatt taaagaaatg ctataaaaac tagaaaacag aggttattga
    13321 gtaaattaga tgaaattgaa aaggcatttt taaacaatga atttgacata ggatttaaaa
    13381 ttgaaaaaga aatagagtta gtagagcaac tagaaatttg catctaagaa caaaaagtca
    13441 cgcaagtagg atttaatata tatatatata tatatattcc tacttgcgtg actttttgat
    13501 gtgaaaatat aaaacttata ccaaatggaa aatcgcaagg ttaaaaaaat tgaccctgaa
    13561 ctttcctaat gtaagaaaat tcaatgtatt ttgaattgga attattttcc actagcaaaa
    13621 tttgataaaa ggtactatta ttgtagtata aatgtttaat tgcgacaaaa aatcgtacat
    13681 gtgttcttgt aatatcgaac aaaagggggt ataattaaaa ttgtaagtta tttttttaca
    13741 aattttaaga atgtattatt ctacttgcat atagggggat gatttggttg aaagaagaaa
    13801 taaaagaaat gttggacaaa gtaggggata aggatatatt agagattatt tatctgtatt
    13861 taaagcaaaa aactaaaaaa taaatatgta catatgaaaa gactatcaaa tttaagatga
    13921 tagcctttta ttctttttga aataattcta gtagcttttc cattgcctct aattctttgt
    13981 catttaactt gctaaatttt aacattgctt tagcaagacg ttcattctta tctattgcgc
    14041 ataagacttc tgctgttatt aagttattgt ttgaaaaagt atacatatcg ccttcgcctg
    14101 tttttagcca tttttcgttt accttaaatg ttgaagttat taattttaaa tcactttctt
    14161 ttattcgtgt tcttccactt tcaagattgt aaatttcgtg tctagtcatt cctagcttgt
    14221 tcccaaattc gatttgagat agcccctctg caattcgcaa gtctctaatt ctagtattca
    14281 ttttaaaact cctttcatag cttaatttta cagtaaagtt gcccgtaagt caacgcaaaa
    14341 aaacgcattg gggcgtagtc ttgcgaaaat aaattagtaa aagttattga cttgcccctt
    14401 attacgcact ataatataaa tatattcgca ataggagtca ggaggttgtt tctatgacta
    14461 gagaagaaag aattttaaga aatgcaattt tgtacttaga tatgagtgag gaaaagcaaa
    14521 gaatagttga ctgtatttta aatgcaaaga caatagaaaa cattgttgag aatgatattt
    14581 tagatgttaa taaactaaat gaagcagtaa aagaaataga acaaattatt tgttaaaagc
    14641 gtactttgac ggctacgcta aaccaattaa aaaaagtaaa aaataaataa ggaggctgga
    14701 aaatgataaa agttatttta tgtagtacta tcgacgagta cattgaatat gaagatgtaa
    14761 taggagcaaa agacagctat ttaatggatg ttatcatagg tggcgaatgt tcagaaattg
    14821 atgctataga aactttagaa attttaaaaa atagtaaagc gagaattgta tcagaagcag
    14881 aaaaaacttg tttaaaattt ggtattggta atcaaatttc aaaggcgata gaacaaggta
    14941 taaaagaggg ttcaaaacta aaaaggagga tacattgtga taattgcggg gcaatcgtag
    15001 acagttcgga agaattgacc tcaatgccag ttttagaaaa ttataacaac aatcaatccg
    15061 tttctgacca actaggaagt atagatgtat gcccaaattg tttgctagaa ataacagact
    15121 tactgtaatt ccctgtttta gttgaaaaga aacaaaaagt aacagcgtaa gccgtcaata
    15181 aaaagaaggg gggtttaaaa agatgattaa tttagaaaca ttgtgtaatg gagaaacaaa
    15241 agaaaagatt gaaaatggat tcatggagat atttaaaaac atacaagacc ctaatacacc
    15301 agcgacatct acaaggtctt tgactgtaaa ggttacacta aagccaggta aaaatcgttc
    15361 tcatgttaat acgcaaatac aagttattcc aaaactcgca agtgtcttac catctgaaac
    15421 ggacattata gttgaaaagg attttagaac tggagaagtt aatgcaaatg agtatggaaa
    15481 ccagttgccg gggcaagtaa aattaggcga cttggagagt aaagaaacaa gtgtaacaga
    15541 agaaacagaa gtaaaagaaa gtgaagaaga taatgttcgt aaatttaaga gtttaaaaga
    15601 cttataaaaa ctaaaaagga gtgtttaata tgttaacaga tttatttaac agggtaatgg
    15661 aagttggagc aagaaaaata gaaacaagag aaatcaatgg ggttacgtat acgactgacc
    15721 ccttgattga ggttcatcct agaccagctg aaatagttag gctaagcact ttagatggtt
    15781 taataggata tataaaatca agtattgata gtaagcttaa aagtggaaaa cttatggttc
    15841 aagttgaaag ccctaaaagt gttatattgt gttctgaact acttgaaagt ggcaagaggg
    15901 atatttacat tgaaagtaga gctttattac ctcatgttaa tttcgattgt tcgcttgatg
    15961 cagagcaatt taacgttatg ttgcaatcct ctttcgtaga tacagaagat aagaaactgc
    16021 ttttaaaagt aagcggaaat gtaaaagaaa caagtgtaaa aactgtgcaa gatgatggag
    16081 tatcacaagt agtcacaatg tctacaggtg tagcaagtgt agaggatgta atcctgccta
    16141 acagagttaa gttaaaacct tacagaactt ttgcggaagt agaccagccc gaaagtgaat
    16201 ttatctttag agtaaaagag ggcattgcat ttaagttaat cgaagcagat ggtggagcat
    16261 ggagacttga agcaataaaa aatataaaag agtacttaga gaagaaatta gaaggtatta
    16321 aaaatataga tataatagca taaaaaattg aagaggctgg actaaataac cagcctcttt
    16381 taaaaaggag tgtttatttt gtataaagat ataacgaaaa tgggagcaat tgaacgtggg
    16441 gaatatgttg gtaatgttgc tcttgaatta aaagaagaaa atcctcatct tagtaaaata
    16501 gatttaattg agttagctct taagaaaata agagaggaaa aggaacagga ggctgggcaa
    16561 tgaaaatagg taaatactta atcgtttccc aggagcaggt ggatagaaat attgaatgtt
    16621 tgaaagaaga aaacaaagta agggacgata agataaggta ttatgagcgt attatgagta
    16681 acaatgaaga attgattcaa attttagaag aacagttgaa agagtataaa agtgaattag
    16741 atgtatatag cttgtttata gattgtattt taaataaaga aacaaggaaa gcgatagcga
    16801 tttataacag aactaaaagc attagaatta agcaaaaatg tcttaattct atacctataa
    16861 aaaagggtta agttgaatga ataatttcct tgtaggaata aatgaggtgc ttaaatatga
    16921 gtgaatttgt gcagtgttgt tgctgtgaaa gaacaataaa tatagaagaa aataaccatg
    16981 tacagtatga aaaagaggct ctaggtttag tttttacact gtatttctgc ttaaattgcg
    17041 tagatgaatt aagcgaaatg gaataatttt taaatgacga atggaggtat ataaatatga
    17101 aaatgaaaaa tgttttaaaa ctatcggcaa tgtattgctt ttgtccaaaa tgtggaagtg
    17161 atgaacttgg agaaggtgaa ggaagtttaa tcgttgatga gtatacattt catagaaagt
    17221 gtaaatgtgg atttgacatt atagtagatg aaaggaatga cgaaatataa tgattttttt
    17281 aggaatgata attttgatta tatttgcatt ttttattggt cgtatatatg aatatagagt
    17341 taatttaaga gaatgtgaga attgtaaggg gggaggttgg aaatgaataa cactacacta
    17401 attggtagac taacaagaga ccccgaattg aaatatatac caggttctgg aacagctgta
    17461 tctactttta caatagcagt agatagagat tatgttaaaa aagatggaac taaggaaact
    17521 gatttcatac ctattgaggt gatggggaag ttagcagaag tatgtgctaa taatttagat
    17581 aaaggtaggt tagtagcggt tgaagggtct ataagggtta atctttatga aaaagatggc
    17641 gagaaaagaa cttatacaaa agttcatact aataaaatta aatttttaga ttataaaaaa
    17701 agaggataat gaaaaagaat gtaagttcga gccgggaggc ttagacccgc aaggtttcca
    17761 ggctatagac gacgatatac ctttttaagt aggtgattaa atgattaaaa aagtagaaaa
    17821 aatttttagt ggttcagtta atctgaattt tatggaagat gacctaactt ttaaaaatta
    17881 tttagcgtta tattgttgca taaaaccggt taaagttcat ggaaataaaa aggttttggc
    17941 tagttctact aaggcatgta gtgcattgaa aattagagag catcagaaaa aagaacgtaa
    18001 aaaggtaaga cttgtaaatg caaaaacagg tgaaataaaa gaaatggcta tagatgaagc
    18061 agaaaatttt ttaaatgtta aagatttgta ccctgtaatt cggagaggaa gacctacaag
    18121 aactggctgg tatgtaaagg atgttagcaa tgaagaaaga tattaaatac aatgttatat
    18181 attttaattc aagaacttta agaatggata aagaaagttt tagcacttta atagatgcta
    18241 gagtttgcaa aaatgaaaag ataaaagaat atgaaaatgt tgaaattatt aaaaagacaa
    18301 ttatagaaaa attgatggta taggaagtga atttatggca aaaaagaaaa ggatttgttc
    18361 atggtgcggt aagtcatttt ttacagagtc aaaaaacgga ttttgctgta aatcatgcga
    18421 aagaaagtat aaaaaagaca aagaaaaaaa cgtaagttct tgcttagttg aagaaaccaa
    18481 agaatattta attgaacttg gaaataagaa aagaagagta gatattctaa gaaaagagat
    18541 agatttttta aaagataaag aattttatag agagataaat ttcaatgaac tagggtttaa
    18601 aatacattct agcccgaagg gcatagatga aatgattatg aataatgagg ataaaatatc
    18661 atttaaagaa agtgaaattg attttataga atatagattg agactaatgg atatttacat
    18721 aagtgaactt acagaagaga agcaacaaat tataaaattt atgtattttt atgatggcgc
    18781 aagtaaactt tctaatgaag aaattgcctc aaagattaaa tgtagtcgca gtaatgttca
    18841 ttataaacac aaggaagcat tgaaagaact tgccgaaatg ctttttgaaa gtggaagtct
    18901 tttgtagaac gaaaaaagac ggtttttaag acaaagttag aacactcttt tacgtttata
    18961 tagtgtatga tattaatatc aagaagttta aatttaaata gatatagatt tgaagcctgg
    19021 caggaatgtt gggcttttta taatgcaaat taatatttta agggggttga gaaggtgaat
    19081 agaagtgggt agtaagaaac cagctaaccc aatcaaagac aaacgtatgg tcttaaatat
    19141 acaagaatac ttaaaagaaa agagtgttag aaattatgtt ttatttgtct taggggtggc
    19201 tacgggctat agagcagggg atttagttaa gttacaggta agggacgtta gaaatgcaat
    19261 agacgaaggt tatttcttaa taatggagag taaaaaagaa aagactaaga acataagaaa
    19321 gaaaaataaa aaacctagaa aagctccaat agttcctaat ttagaaagag tactcaaaag
    19381 ctacatacga gataaaaagg attatgaata tatgttccca tccaggcaaa aatctgttac
    19441 tccgcatata ggtgttgaga gagtaacagt gatactgaaa gaagcaggta gatactttgg
    19501 acttaagcat ataacagcgc atagcatgcg taaaacatac gctcgcacca tatacgaaga
    19561 aagtggattt gacattataa gagttaagga aatgcttgga cattctagta tagaagaaac
    19621 taaggcatat ctaggtctta atgaagaaca atatcaagaa tatagcatgt ttttaaatga
    19681 cctaataggg tgattttttt tattttgtct ttttgaatgt gggaaaaata aaggcttata
    19741 tattcaatac atttttttcg ctatatataa agtaggaaaa gtaaaaagtg aatgtctgat
    19801 tctctaagaa aaccccatat tcgttaaaat aggcgttagc cattataaat actgggatag
    19861 aagctattta acgttaaaat gggtatggtg aagcttaagg acatgcggac aaaacttaaa
    19921 ggacatggaa aaatagagaa tgagattgat gtaataaaaa ctataaaatt cacaggcaaa
    19981 caattcaatt acgccctttt acaagagtaa tgacaatatt tataagtact tttgattcat
    20041 ttaaaaaaat tcactcgtat tcataactgt acggtattga ctacgattta attatgtagt
    20101 aaaatgaaca tatagaaagt agtcataact aatggtcaaa agtaaggggt gtatttttat
    20161 gatatttggg tattgtcggg taagtagtaa agtacaaatt gataataata gtttagaaca
    20221 gcaggagcag gaaataaaaa gacattatag tgatgctaaa atatttaggg aacaacatac
    20281 agggtcaacc acgcacagac ctatttttga ggaaatggtt aatcaaatgc gtgatggtga
    20341 taaattagtt gtaactaaat tggatagact tgcaagaaat gcaacagaag gcattaatct
    20401 tatacaaaat ttatttgata gtggtatttc tgttcatgtg cttaatgttg ggcttttaga
    20461 aaatactact cttggcaagt tttttataac aaccttgctt gcagttgcag aaatggaaag
    20521 gaatctaatt ttagaacgaa cttttgcagg taaggagata gcaaagcaaa atcctaattt
    20581 ccgggaaggt agacctaaaa aatatactaa gatgcaaatg gaacatgctt tagagttatt
    20641 aaaaactaat tcttatacgc aagtagagaa aatgacaggg attagtaaga gtacattgca
    20701 aagagcaaag cgaaaacaag aagactagat acctagtctt tttttattga aaggagttga
    20761 tttgtatttc aataaaaaat tccaatcgag aaaaagcgtt tgagatttac aaagagaaaa
    20821 atgggagaat aaaattaatt gatatctcta aaatgttaga agaaaaaagt tgcaatatta
    20881 gtcgttggaa aaaaattgat agatgggatt atagacttgg aataaataag aaagttggcg
    20941 caccagccgg caaccaaaat gctttagggc atgaaggagg cgcaccagtg aataatcaaa
    21001 atgctagaac tcatggattt ttctctaagc ttttgcctgc tagaacttac gagattgtta
    21061 aatatatcga gaaggatggt ggtaattctt tagacattct ttggaacagt atagtagttc
    21121 agtatgcgaa aatattagat tcttttaaga ttacccatgt caagaataaa aaagaccata
    21181 caactgacat taagaaaaat ggtaataaaa ttaaagagta tgaaattcaa cactcttggg
    21241 ataaagttac aaatgctatg aaagcagagg cgacagcatt taaagctttg tcaaaaatga
    21301 taaaagacta tgaggaacta cttcataaaa attgggattt agcaacagaa gaacaaaaaa
    21361 acagaataga aaacataaaa gcgagaacaa ataagttgac tggtaatgac ttagaaatag
    21421 aggatataga agaaatagag gcagaaatat atggcagtaa ctaagaagaa aactatacct
    21481 tttttatttg gagataagca caaagaatat attaaaaagt gcgctatcaa tacatacaat
    21541 attgccgaag gtgctgtaag ggcaggcaag acggtagaca atgtatttgc ctttgcgcat
    21601 gagataaaga catctaaaga taagatacat ctagcaacag ggtctacaag tgcaaatgca
    21661 aaattgaata ttggagatgc taatggtttt ggacttgaat atattttcag agggcaatgt
    21721 cattggggca aatttaaagg caatgaatgt ttatatataa aaggtatttc aactaagcat
    21781 aagcaaaaga ttgttatttt cgctggaggt gctaaggctg atagttataa gaaaataaga
    21841 ggtaactcgt atggtatgtg gatagcaaca gagataaatc tgcaccatga taatactatc
    21901 aaagaggctt ttaatagaat tatagcatca actaatcgta aaattttttg ggatttaaac
    21961 cccgataatc ccaactcgcc aatttacaaa gaatatattg atagttatat gaaaaaagat
    22021 agggaaggca ctttactagg tgggtataac tatcagcact ttaccatcca cgacaatata
    22081 acagtctcgg acgagagaaa acgggcaatt atatcccaat atgacgaggg tagtatctgg
    22141 tacaagagag atatactagg tcagcgctgt gtagctgagg ggcttatata tgaatacttt
    22201 gctaataata aagagcagtt taagactgaa acaattgacc cactcatgga tgttattgtt
    22261 ggggtggatt ttgggggcaa caaatcatat catgcttttg ttgcaactgg aattacttat
    22321 aactataaaa aagtaatagc tcttgcaagt gaaagaccta gcgcagatac cagtccagat
    22381 gagttgaatg ctttacttat aaattttata aaaaaagtaa taaatctgca tggtaaagta
    22441 gattgtattt actgtgatag cgctgaacag gtactaataa aaggcattaa aaatgcagta
    22501 gaaaaagaaa atttaaatgt tagtgttaga aatgctttga agaacgctat taacgatagg
    22561 ataagactta cagacttatt aattagtcaa ggtaggtttg agtatacaag gtatagcgag
    22621 actcttgtta gcgcattatg tggtgctgtt tgggatagca aagaaactaa tgaggatgta
    22681 agacttgatg atgggtcaag tgatatagat agtttagatg cttttgaata tacgatagag
    22741 agatacttga aaaagtttat aagaggtgac taaatgtttg aaggattgaa aaatgctata
    22801 aggggggtga ttaacaagtt gtttaataag agttctatac aaaatgaatt aaaggtagat
    22861 atagctgtta gtgataagat gtcaagagca attgacttgt ggatggctat gtacaaaaac
    22921 aaagccccct ggctaaatga agtgactaaa agtttaaatt tatctgctac aatttcaagt
    22981 gaagttgcta ggcttgttac ccttgaactt gaaagcgaag tagtcgggaa tgacttttta
    23041 aatgaacaat atcaagacgt attaaaagat ataagaaagt attgtgagta tgcttgtggg
    23101 gctggcgggc tggtatttaa accatatgta agcggaaaca aaatagaagt tgattatgtc
    23161 caggcaagta atttttttcc tacagaatat agttctacag gcgatattac atcagctatt
    23221 ttttcagaaa taaaagttaa aggtgatatt aaatacacta gattagaata tcataattgg
    23281 acttctactt cttatactat ctctaattac gcatataaga gtaatacagc aaagttgatt
    23341 agctattcag atgatttagg gaagggaatt aaattaagtg atgttccaga atgggcagag
    23401 ctttcagaag agttagtgtt agaaggaatt gaaagacctt tatttgcata ctttaaaatc
    23461 ccacaggcta atagtttaga aaactcatca cctttgggcg tttctgtttt ttcaagagca
    23521 gttgacttaa taaaagaggc tgataagcaa tattcgagga ttttgtggga gtatgaggct
    23581 acagagatag caattgatgc agattctagt atgttcagaa gaaacgcaaa tggagaatat
    23641 gaagttccgc atggcaagaa acgactgtac aggatgttag aaatggagga tggagacaat
    23701 aaatggaatg tgttttcacc agccataagg gattcaagcc ttttcgctgg gcttaatcaa
    23761 ctactaagaa aaatagagtt taattgtgga ttgtcatacg ggattatatc agatgcacaa
    23821 gagactgaaa aaacagcaac agaaataaaa actagcaaac aacgtttata ctctactgtt
    23881 aaggatatac aaaagtcttt agaggacgcc ctagagggat taatttattc tatagataag
    23941 tggtctattc tggcaggatt tactcctagt ttgaaatatg aaacgacttt caattgggac
    24001 gatagcgtaa taatagacaa agattctgaa ctcttagcaa tgcaacagga tgttgcgtca
    24061 gggctgatta gaccagaatt atatattatg aaaaaatatg gagtcacaga ggatgaggct
    24121 ctgaaaatga tgcccgatac tagggaatta gtagaggata atccttttga caagggtggt
    24181 atgtagatgt taacatctaa tgagttacaa gaaattccta agtattttgt tagtttattt
    24241 caaactttag aggactttgt aatagcagat attgcaagac gtattgcaaa ggctgggaat
    24301 attacagata tggcagaatg gcagttaata agagcagaag agattgggat ggctgataaa
    24361 gtaattaaaa tgaaaattgc tgaaatactt gatatctctt ttgaggcagt tgacaagtta
    24421 tttgaagaaa atgctgtaaa gtctattgaa agtgatagtg ctttatatga acatgctaaa
    24481 ttaactccat tgcacttgaa tagttctgaa gaacttaaga actatgtttc ttcagctaaa
    24541 gagcaaacta agggtaaact gaagaatatg acaggtacat taggattttg tactattaaa
    24601 aaaggtaaag ttattagtaa gaaacttaca gacatatata tagagtcttt agacttagcg
    24661 caatttcaag tcagtacagg agtcttggat tataagacag cagttaaaaa agcagtcaag
    24721 aggcttgcag atagtggttt aaggtttata gactatgaga ctggttggac aaatagaata
    24781 gatgttgcaa ctagaagggc tgtgctgact ggtgttaatc agatgtcgca gaatattaat
    24841 aataaagtca ttaatgattt ggatactgat atagtagaag ttacagcaca ttcgggggca
    24901 aggtgcgagg gagcaggtat taaaaatcat aagaaatggc aagggaaatg gtatagtttg
    24961 agtggtaaaa gtactaaata tccttcctta aaagtggtta cgggctgggg gcaaggtgat
    25021 ggtctggggg gctggaactg ttcgcatcaa ttccatgcgg ttattcctgg catatctgtt
    25081 cctgcctact caaaagagca attaaaaaat atagaccctc ccgatattgg atataaaggg
    25141 cgaacttata cgcattacca agctttacaa tatcaaagaa aaattgaaac atctatgagg
    25201 cagacaaaga ggcagttaat cgcttacgag tcggcagggc ttaaagatga gtttacaaat
    25261 gcaagtataa agttgcaaag acagaaacaa gagtatagag agtttagtaa gattgctaaa
    25321 atgaggttgc agaatgagag acaccaggtt tttggatata ataaaagcgt cagccagaag
    25381 gctgttcacg ctagtaaaaa ggagagaaaa aatgggtaaa tatagaaaga aagcaattat
    25441 tgttgaggct tttaaatggc taggaagtgt agaacaaaag gaagaaccga catggatagt
    25501 tgaggctatt aaaagtggta atgtttggat tgaacaaaat ttaggggaat tatctccacg
    25561 catgtatata aagacgcttg aaggtataca tgaggctaat gtgggggatt acattgttca
    25621 agggataaaa ggagaattgt atccatgcag ggctgatata tttagagaaa catatgatga
    25681 agtcttatat cgataaggct tatttttatg cttaaaaata cttaatttat ttatatagat
    25741 gtctactaag cgacgtaaaa agttagacaa tacgtgatgc taccacgtaa aaaagcgtag
    25801 ttgtaaacga aagcaggagg tttttatgaa aagagagttt ttgaaagatt taaatttaga
    25861 ggatgaggtt atagaaaaaa tcatgtccga aaatgggagg gatatagaaa agtataaaaa
    25921 agaagtagaa aagaaaaaag aagagttgga aagcaaaaat actgaactcg aaacagctaa
    25981 taataaaatt aaagacttag aaaaaataga tgttgagagt ataaaaaaag aggttgatga
    26041 ctggaagagc aaagccgaac aggctcaaaa agataaggaa ttgattgaaa atcaaatgtc
    26101 agagcaaact tataacttaa atttagataa ttatttaagt aattttaagt tttctagtaa
    26161 tctttcaaaa gaggctgtaa aaataaaaat gaaagaaaaa ggacttgaat ataaggatgg
    26221 ggcgtttgag ggagcagatg attatataaa agaattgcag gcaaatgacc caggggcttt
    26281 tataagtggc aatagtgttc caaagattgt tagttctagc ggtggagatg ctggagagtc
    26341 taaagtaagt ttaatggaac aaatgatagc aaaaaataga gaaatgttga acatatagga
    26401 ggtagaaaat gagtttattt gattcaaaaa tttttaatgg agaagtgttt ggaaaatacg
    26461 ttgagaccgt acctaatcta aatagggacg aattaataag gtctggagca ataagaagaa
    26521 gggacgattt aaaggctatg ttttcagccc aatcgggagc aaattatgca actattccaa
    26581 tgcttggctt gattgatggc gacccagtca attacgacgg acaaacggat atagtagcaa
    26641 ctacaacaaa gaccttttcg cagtctgtaa ttgttgttgg tagagctaaa tcgtgggtgg
    26701 aaaaagactt tagccaggac gtaaccggcg gagttccttt tatggagaat gtaggaaatc
    26761 aagttgctaa atattgggat aatatagacc aaaaaacttt gttagctgtt ctggaaggta
    26821 ttttcgcaat gacaggggcg aaaaatttag aatttgttaa caatcatact tttgatataa
    26881 caaaagaagc agaaacagaa aatcaaaagg ttaaagccga aactcttaat agtgcaactc
    26941 aaaaagcttg tggagataat aaagaaaaat ttgcacttgt tataatgcac agtgttatat
    27001 caactaactt agaaaattta aatcttgttg ctaggttaaa atatactgac ccacaaggta
    27061 ttcaaagaga aatgaaatta ggaacttggg gtggcaaatt agtcttaatt gatgacaata
    27121 tgccaactag agaggtcgct gaaagttcag agggttcaaa tgatggatac acagagtata
    27181 cgacatatgt gcttggggaa aattctattg acttcgagga tgttggtgca aaagtcccat
    27241 atgaaatgga tagagctcca ggtaaaaatg gtggagaaga ttacttatat agtagacaaa
    27301 gaaaagtatt tgcgccatat ggtattagct ttactaaaaa agcagttgca acagcatctc
    27361 caactgattc agaactgaaa aacggagcaa attgggagct tgtgaatgat agttcggatg
    27421 ggtctaaaac ttatatagac cacaaagcta tacctattgc tagaattatt tcgagagggt
    27481 aggctatgaa tatgtatata gactatgaat tttatagcgc tcttggggga gaaatccccc
    27541 aggacgaatt taataaatat gcaataagag caactaagta tatagattat aatactttta
    27601 atagaataga agaagttaca gaagaaataa aaatagccac ttgtgagatt gctgacctca
    27661 tatataaaag caacctggaa ggcaataagg aagttcagtc tgaaagcatt ggaagtcatt
    27721 cagtgactta ttcaactaac aataaaacga tagagcaaaa agcatatgat attttaaaaa
    27781 tgtatttaga ttcagatttg ctgtatcgag gtgtttagtg tgttttttaa agataatata
    27841 actttatata ataaatatta tgataagtcc tctgataaaa ctttatacaa gaggacttat
    27901 ctatttgaag tagattacca agaggctaaa aatatagctg ttacagacaa ggggttgtta
    27961 agtgctgata gtgttaaggt tgtagtcccc ttttctgtaa atgcaagagg gaagaaatat
    28021 atagaccctt ttaaatatta tgaactagaa gaaaatgaaa agaataaatt ttatactttt
    28081 aaagttggag atattattgt gaaagaggtc gtagactttg aaataactag cgttaagcct
    28141 tacacattaa gagaattaca aagtaaattt gatgatgtta gtattataaa atcggtcatt
    28201 aaatgcgatt ttggaagtat aagaatgaga catttcgaat tggaggcaga ataatatggg
    28261 atgaatgttc atgtagatat taatgcaact agaataatga gagcaaaagg gcttggaaga
    28321 gggggcaagg cacaaaagtt tttgactcat gaagttagaa ggctttcaga ctcttatgta
    28381 cctaaacaaa gtggaacgct gaaaaataca gctagagaag aagttgacaa gataatatat
    28441 atacaacctt atgcaaaacc gcaatattat aataatgcag ggtgtggctc ggagggtatt
    28501 ggagcaggag ggaaaagggg caagcattgg gaaaagagaa tgtacgcaga caaaggggac
    28561 gaattaattg ggtctgttgc taaatttgtt ggaggtagaa gggaatgact ttagttgaaa
    28621 gtgttagaga gtttataaaa aaatgtccct atttggatga atttgcaaaa agtataaatg
    28681 tcgagttttt aggtgaggaa tttacttctt atacattaga gactgttcca tcagaaacag
    28741 tcattaaaag atttataaat ggcgactcta taaaacagtt tatatttata tttgcaagta
    28801 gggagtgtta tgggtctgat gttatgcaga atatagaaaa tagccagttc tacgagcaat
    28861 ttgcagactg gatatataga gaaaatttaa gtgggaattt gcctgttttg tctggtgata
    28921 aagaggcaat gtcactagag gtaagcactc caggataccc agttcaaacg ggtatagata
    28981 cagcacaata tcaaatacaa ttaaaattaa aatattttga aaaaggagaa atgtaaaaat
    29041 ggcaggaatg gaaacgatac aaagatatag agtagcagac tacttaggag ttttggggga
    29101 tgatggacaa aattcctggg tattaatggg agcaggattt aatacattag acgaaaaccc
    29161 atccgcacaa agtgactcta aaacatatat aaacgataga aacagtacta gcgctatcaa
    29221 atcttatcaa acgcaatttt cgtttgaaac tgatttaatt aaaaatgaag aggcgataat
    29281 ggaactttac aaaataggta gagaccaggc aactggcgca gatgctgaaa gagattattt
    29341 aagagtagaa ttatttttac caattaaagg caaagaaaat acttttaggg ctagaaaatt
    29401 tagagtgtct gttgaagtga gtggatttgc tggtgcaggt ggagaaacta ttacagtaag
    29461 tggaaattta aataccgttg gcgaccttgt acatggagaa tttaatactc aaacaagaac
    29521 ttttgtagaa gatggagcag aagccccgcc agctgaaagt ttaaaagttt taactataaa
    29581 ttcagttgca ggtactaaca cagggaatac aaaaataaca gttactccaa ctcttgaaag
    29641 tgggaatagc tataaatata aaactggggc aaatattaca gttccaacac ttgatactga
    29701 ttgttcagtt gggtacactg cttgggatgg tgtagaagaa ataacagcaa ctaagggaaa
    29761 taaaatatta atagtagaag ttaatgctaa taataaagct aagaaagctg gtattactac
    29821 gattacagta aaagaataag agggggctaa ggaaatgata attaataata tagaattaga
    29881 agatttagaa atatatgatg ctgatgtcat ggaaagagtt gaaacgtcaa taaaaacagc
    29941 tatagacggg atacagattg ctgaaacaga ggaaaaggaa aatcatgcaa tttttagaag
    30001 aatatgtgat ttaatttttg catgctttga taatattttt ggggataata catcaaataa
    30061 aatatttgct ggaaagcgta atgtaaaagt ttgcatgatt gcattttctg aattagctga
    30121 cgaagtagag agacagaaaa aagaaggcat ggagctattt agttctattg gtaataagta
    30181 ttcaccaaac agaactcaac gtagagctaa aaaataatga atatgttagt cgatatactt
    30241 cccgaaagtg ttgaaattga tgggaaagag tataaaatta atacagactt tagaatatca
    30301 attttgtttg aaatgttaat acaagataat tctataagtg acgaggaaaa gggggaaaat
    30361 gcacttcttt tatattatcc tgtaatccct acaaatacca caatggctat agaaaaaatt
    30421 atttggtttt attcatgcgg gaaaaaagaa aacaatgtgg atgatggttg ccgaggtggc
    30481 gcagggtcta gcaaaagtca aatatattca tatgactatg atgacgagta tatatattca
    30541 gctttcttgg gtcaatatgg gattgactta caggacatag aaaaattgca ttggtggaaa
    30601 tttaaagcat tgtttaagtc tttaaaagag gacaccgaaa tagttaaaat catgggatat
    30661 agggcaatgg atgtaagtgg agatatgcca aaggctcaaa aagattttta tagaaaaatg
    30721 aaaaagatac atgctatacc acttcctcaa aacgaggttg aaaaaataaa cgaaattgaa
    30781 aaagcattgc taaatggtgg agatattagt aatttattat aaaataatat actctacacc
    30841 ttccgaatta atatataatt agtatataaa tattttaatt tgggggggat tgttatggga
    30901 tttaaattta gaaaaagcat aaatctaggc ggtggattga agttaaatat taataaaaat
    30961 agtgtcagtg ttagcggtgg cgttaaaggt gctagagttt cagttaattc taaagggaaa
    31021 gctacaacaa ctttaggagt ccctggaagt ggcttatatt accaggaggt agctaatcta
    31081 ggtggaaata agaagagaaa tagtaagagt gtaggaagtt cagagagtag taggcaggct
    31141 caacagctat taaaaataat aaacgattgt gctaatatag ttaatactac aaaagaccct
    31201 aaaacatttt tttatagata taatatgttg ttagataagt catatgcctt agttgccata
    31261 gaggatgatt taaacttttc gggtcaatct cctagtcaga tgctagacag cataatagca
    31321 aaaaggactg atactataaa cgactttttg actagatact acaatgaaat gtctagtaaa
    31381 ttgaaagaac tgaaaactga aaaggctcgc atgaacaatg cttataaatt ctctagctta
    31441 gcaatagatt ataaagaaca tttaaataat gataacaaag ctaagcttaa tagactatat
    31501 aaacaactat tagatgatgt taaaagacaa gattaatatt tataaaaaga aaaatataat
    31561 aaaagattta aataaaaagc acttactttt taaaaaaagt aggtgctttt ttaatgcaaa
    31621 tttacaaaaa aaaagggggt ggatagatgg cagatggttc tattattata gatactcttt
    31681 taaataccga agacgcagag aaacaactta ataacttagc tgataccatg aaaaagaagg
    31741 cgaaaagcgc tttagcggta gctggaattg gcgcaagtct aggagctata ggaaaacagg
    31801 ctattgactt cggagatgaa tatcaaaaag caatgaatag tttcgagtca gcgacaggaa
    31861 acgcagaagc taaagcaaaa ggctttggag aagcgctaca gcaggtttat gcaaataatt
    31921 ttggcgagga tatgggcgat ataagtgagg tcatgagttt aatatctcaa aagcttgatg
    31981 ggatagatgc tagcaatatt caagaggtta ctgaaagtgc tattatgatg agggatacct
    32041 tcgatatgga tattggggaa agtttaaatg gtgttaactc tatgatgcgc cagtttgggg
    32101 ttagcgcaaa agagtcttat aacttgattg cacagggtgc gcaacaaggg ttaaatcaga
    32161 atgacgacct cggcgaccaa ttagcagaat attcgacata ttacgcacag atgggttttt
    32221 cagcagagga aatgtttaat atgatgaaaa acggggcgga gagtggagtc tatcaaatag
    32281 actatttaaa tgatgccttg aaagaattta atattagaac aaaggatggt tctaatggta
    32341 ctaaggaagc ttttgaggcg ctcggtttta atgctgatga actaactaag aaatttgcga
    32401 atggtggaag tagtgcaaaa gaagctttta ttcaagttac aacagctttg aataacttgg
    32461 atgataatgt attgaaaaat cagataggtg tacaactctt tggtactaaa tttgaagatt
    32521 tggaagcgga cgcagtaaca gcgctaacaa atattgaagg aagtataagc agttcaaaag
    32581 ataaattaga agaaattaat aagataaaat ataatagctt cggagaagca gtaacgggaa
    32641 ttggaaggca aatgcaggtt aatttacttc ttccaatcgc agaaggattg cttcctgttt
    32701 taaatgattt agcaaataaa tttgctaagg cttttcagaa tgagaatact aaaaatgcta
    32761 taagaagtac agcagaaagt attggcgggc taattggaac agttgtaaaa ttaatatctt
    32821 tagcattaac ccctttaatc aatgttataa cctttttggt tcagcatggt agccttgtag
    32881 cacccttaat agcaggaatt ggagttgctt ttacaagtct taaagtagct gggattgtga
    32941 ggggtataca aaaaagtttt acagatgccc agttggcggt aagaattttc acatctggcg
    33001 tgactgagtc agaaactaaa ttgactttgt atcaaatggt agttggattg gttaccaaaa
    33061 aaataagttt ggcgcaagtt gctaccaatc tgtggaaagg agcattaact gcgcttggtg
    33121 gtccagttgg cttaactata gttgcggtaa ctgcattaac ggcggtcgtt gtttctttat
    33181 ggaaaaataa cgaagctttc agagacgcgg ttataaaaat ttggaacaat attaaagacg
    33241 ctggagctaa aatatttggt cagattgcta aattctttac agaaacaata ccacaagcac
    33301 taagtaaggc tatatccttt attaaagata attggcaagg attattgcta atgctggtta
    33361 atccttttgt tggagctttt aagctgattt atgataattg cgaaagcttt agaaataaag
    33421 taaattctat atttaacaaa atcattggta tctttaaaaa tgttttaaac tttattaaaa
    33481 acaactggaa aaatatagga agcatgttat taagtccttt tgtaaatgca tttaaagcta
    33541 tttataatag ttgtgtaaat tttaaaaata aggtttttag ctttttctct aatgttgtaa
    33601 aaggcttcgc tacttttgga aaaaatataa tagttggaat aataaacgga ctttcgagcg
    33661 gtatcggctt ggttgtagat actgtaaaaa atatcggttt aattgtagtc aatacattta
    33721 aaaaagtgct acgaatacat tcaccgtcca aagttacaac agagcttgga gagtttgcag
    33781 gtattggttt tgctaatggt ataaaaaata caagcaaaga tgtagtagaa gcaacaaaag
    33841 aattaataag cgctatggaa aaaaatctcg ataccaataa aagcaaattt gaaaaaattt
    33901 gcgaggctat aacaacagca ttaaaaaatc aatacgaaag tcaaaaagat atacaaataa
    33961 aagcgcttga tgaaagacta aaagttgagg aaaaggcatc aaatgataga ttaaaggtct
    34021 atgaaaaaga atacaatgag aaattaaagt atttagatac agaaacaaat gagaaaacta
    34081 atgctataca agaacaaatt gacgccatag acaagcaaat tgaagaagaa caaaaagcag
    34141 aagaagagaa agcgtacaat gaaaaaataa gcgaccttga caggaaacta gcaacagcta
    34201 aaaatcaaaa agaacgagaa aaaatacaaa aagaaatatc agaagcacaa tcagatagac
    34261 agaagaaatt attagatgaa caaaggcaac aagaaaaaaa cagattaaaa gaacagattg
    34321 aaaatataaa aacagaagct agtaacaaaa aagagcagta caaagaagag tacgacaatc
    34381 aaaaagaagc agaaagtaaa aaacttgaat tgataaaaga gtctaatgct agtcaaaaag
    34441 aagaaattga aaaatatttt agtgaacttt tagaagagac aaatattcaa aatgaagcga
    34501 gaaggctact tttacaaaaa aattcagaag aaattataaa acttttaagt gaatataacc
    34561 cgcattggca ggatgcaggg cagagtttag cagatagttt gttaaatgga gttaactcta
    34621 aaaagcaatc tatccaggaa gctgtaaagg aagctattaa cttaaaagag attataccag
    34681 cacaagaaaa agagcttgat agattgaaga aaaagttaga agaatatgag aaactaaaag
    34741 aaaaagctaa tacttctagc gcaagtggag gggatgtaga ctcatctgct aaaagttcct
    34801 ctttggattc tggttctgcc aaactggacg ctggagaact agaagaatat gctgatggca
    34861 tagatgatgt taaaacttct gttgaaggct tagaacttgt cagtgatgaa ctcgtaacag
    34921 gaacagtccc taaagttggg gattcagcta gtaaattatc tgaaagtata aaaaaagggt
    34981 tcaatggcat tggtggtttt tttggtggaa tagaaaaatg gctaagagat acagacaaaa
    35041 atataaatga gtggctagct agtgcaaaaa attctattgg tgatttcttc tctaaagtaa
    35101 aagataaatt caaagaagga attgaaaata taggtggatt ttttggtggt cttggaaata
    35161 aaataagtga aggatttgca ggagctggag acttttttac agagtttgga gataaggcac
    35221 agcaaggttt ttcaagtata aaagataaag ctgtagagat tttcggcggt cttagcaatt
    35281 ggttcaaaga gactgggtca aaaattggag atgcaattgg aagcggatta aaaagtggtt
    35341 tagacttttt tactagcaca gtaccaaaat ggtttgcggg tattggcgac aagataaaag
    35401 aagaatgtgg gaaaatagga gaaaaaatat caagtttctt taatgagaca atgccagcta
    35461 taataaataa tattgtcgaa tggtttaaac aaattccata taatattggt tttgtaatcg
    35521 ggtctattgc tggttttttc gttgatttag gagctaatct gtacgcctgg gcaacagaaa
    35581 cattaccaga aataataaat agcattgtcg aatggtttgt aagccttccg gaaaaaattt
    35641 cggaattttt tagtatcata ttgcaaaata ttcaaacttg gggcgaaaat ttcaaggttt
    35701 ctgtttcaga gttttttggt gcaatttggg aaaatataac acagtttttt acagagctac
    35761 ccgaaagaat ttcggaatgg tttaatacag tagtagaaac aataagtggc tggggcgaaa
    35821 atttaaagca attggcaaca gatattttta atcaatttgt tgacaatgta ttaattccaa
    35881 tttcggagct tccgggaaaa ttatgggaaa agtttacaga tatcatggga aaagtttcgg
    35941 aatggggttc taatctaatt caaaaagcat tagaaacagg aagcaaattc ttagaaaata
    36001 taatgaaatt cttttcagaa cttccgggca aaatatggga aaaattaact aatataatgc
    36061 aaaaagtcat ggagtgggaa gttaatttat ctaaaaaagc tttagaaaca gcgaaaaact
    36121 tttttaacaa tatatacaat actgttagcc agcttccagg aaaatttaga gaatggctag
    36181 acaatatcat ttcaaatgtc ataagctggg ggactaactt agtcaaacaa gctggagaag
    36241 ctggaaaaaa tatggcaaat gctgttaagg atgctttgaa agatttgcct agtaaaatta
    36301 tgagtatcgg gaaagatgtt gtaagaggct tatgggaagg tataacgggc atgggtggct
    36361 ggttaaaaga ccaagtcctt gattttgcta gtaatgttat agatggattt agagacggct
    36421 ttggagtgca ttcaccttcc ataatcatgc gagatttaat tggtagaaat cttgtaaaag
    36481 gggtcggcgt tggaatagat gtagaaacgc caggcttgaa agaaaaaata gagaaaaata
    36541 tatctgaact tacaggtaaa ttaaaagcta cagttaattt tgaaacgtct aaaatacaag
    36601 ctaatatagt tgcaagtaca gattttaaag ctggaaaaga aacagctata atgagtaata
    36661 ataaggctga tgaagctaat gaaaatcaag caggtattaa acttaatata gaaaactttg
    36721 taaataatag acaacaagat atagaaaatc tttttgatga aatattgttc ttagcaaaaa
    36781 gaaaaggagc tttataaaat gttccttttc ttttttatat ttccttttat cttttttgaa
    36841 aagactatag taaaaaatat aactacaaaa acggagttat gtctaaaaaa taataaatga
    36901 gaggagggca taaagctatg tttttcgttt ataacggtcg agacagcaga gaatttggtt
    36961 taaaaatata caatataaac gacttatcag ctccacagat ggaagttgaa agagtgagtg
    37021 tgccaggcaa agatggagat ttactactaa aaaaaggttt tgaaaatttt acgcttacaa
    37081 tagaatgcga tatagatgca agacaaagta atatagaaga agttgcaaca gaaataaaaa
    37141 aatggctaca gggcgatata tcatataaaa aacttttttt aagtaatagt gacttttatt
    37201 atctagcaag ctgtaacaat aaactagata taactagaaa ttttaaaaac tttgcatcat
    37261 gtcttttaac attcgactgc taccctttta gatacgcaga agaagaaatt ataagtttaa
    37321 atgtattaaa tctaaagagt gctactataa caaatttcta tagagagtca aaacctgttc
    37381 tctatataga aggagcaggg gacataagta taaaaataaa tactcaaagc atagtgctaa
    37441 gaggggtggc agaaaatgga attttaagcg acttaataat agatagcgaa cagatgaatg
    37501 tatatagaat aaacaaagaa aataacatta ttgtaaatga aaataataag ctttttagtg
    37561 actttcccat acttgaagaa ggagaaaatc agattagctg ggaaggggat ataaaaagca
    37621 taaaaataaa tcctagatgg aatattttat aaagttttaa attaaaattt ggagggaggg
    37681 attaattatt caaaaaggaa aattagtttt atatgaagaa aaagaaacag attttaggca
    37741 tttaggttta ggtgttctta cgaatgtaat aaatgaccat gttagagagg aagaaagcgg
    37801 agttttcgag ttagaattta ctatctacga agactctttt ttatttaaag aaataaaagt
    37861 agatagatta attaaagctg atacttcgcc cgactacaaa gaccagcttt ttagaattta
    37921 ttatatatca aaaaatctag gtgggtatat taatgtaaaa gcgcaacaca ttaagtatga
    37981 tttgcttaat aatttcatag aaagtttgga attgaacgat attacttgcg aagaagcttt
    38041 agaaagagtc tttcgtgctt gtgaggaaca gaacagattt agaggacatt cagacatcaa
    38101 ggcaaggaat actattaata ttgaaatgca aagcccttac agcgcaatct gcgagggcga
    38161 aaattctcta attcaaaaat ttgaaccaac tgcgaaactt ttctttgata attttaatgt
    38221 tttcttaaat tatcaaagag gtgagagtaa aaatgtgcta cttgcatata aaaaaaatat
    38281 aacagggctt gaagcggagt atgatacaca agatattgta acaaaaatat atccttatgc
    38341 ggtctacgaa gatgaaatga ttacattgac agaaaaatac attgttagtc ctaatatgaa
    38401 taaatatgct acccaaaaga ttgttgctat agattttagt tctgatgagg tcagtgttga
    38461 agaagaatta agagaaaaat gcaaagacta ttttaaatac aatcaagttg atttaccaaa
    38521 agttttatac aaagtcaact tcgtagattt atctacaact ataaattata aagattacaa
    38581 aatgctagaa acagtcaatc taggtgatga agtgataata cgagatttta atttaaacat
    38641 taacgcaact gctagagtag ttaaaacaga ctatagccca ataaatcgaa aatactatag
    38701 tattgaagtt ggcgatttaa taaatcattt agacttttta aataacaagt ttaataatat
    38761 agaaaataaa atagaaacag taaaaaaagc tattgataat gtcaaagtag atgattcaga
    38821 gtttccagac actttaccga atacaccagc tttgacagca gaggggttat tcgcttttat
    38881 caatctaaca tggacattta caaataagtc ttattacaaa tatgaagttt atgcgtctca
    38941 aattaaagac tttgtaccag atacaattaa ttttacaaat cgaatttttg tagggcaagc
    39001 aagtgcttac gttcatcaag ctgaaccttt tcaaacttgg tatttcagag taagagcagt
    39061 aaactcgcat ggaaatgtta cagagttctc taatcaagta gaagcacaga ctacaaaaat
    39121 cgacgatgga actatgtgga tagaaaaagg agcaatagca gatgcattaa ttggggagtt
    39181 gaagctagat agaggttggt ttggacagct aaaaggcatg tatataaatg ctagagaaat
    39241 ggtagtcgta aatgataatg gagtcaaaac aatggaaatt gatagttttg gagatgttca
    39301 ttttaaccca aatactttta aaattgcttt taatagtgta agtccttatg ttcttattga
    39361 tgatgaagga ttgaaactta taagcgggaa gggatttaca aaaatgaccg actcggggct
    39421 atatacaaaa ttctacaccg gttcagatgt aacagagtca tattatcttc aagaaagtgg
    39481 atacacatac tttgattata aagattcaaa aatcataata gacttaccaa aaaaatttgc
    39541 aggtaaaaat tttaaagcaa cttgtgcaat aaggtctaca tcaacaaatg gtagctgttt
    39601 aagttatttc agcacatcag cgacagcaaa aaaaagtgat acaaatccac aattggaact
    39661 ttctgcatct gtgcgaggtg tgtcttacga aatgtcagga agccaaacaa taggctactg
    39721 gctcggaatg aatttttttt cagaaggatt tattacagta caatactatg tttatgctta
    39781 aaattttcaa aaaaaaggag aagaagtatg gttactattt tttacagcaa gagaactgga
    39841 gaaatatata attgcataaa atcagagatg gagcaggatt atacctgctt tgcagatagg
    39901 gaagaggatt ataggcaaat attagataaa atagttgtcg aggatattcc agaaatttta
    39961 aatcctcgtg attataaaat tgaaaacgga aaatttgtat taaaggaaaa agaaataata
    40021 aaagaattta ctccaaaaat ggtagagtaa aaggagtgat aaatttgaga gatagaattt
    40081 atacagtaga tattaacact aaaagttacc aagttgcaaa atataagcaa tatgataatg
    40141 caatagaatt taagattaat ttattagaaa ataatataga aaaagatttg acaggatata
    40201 cagctatagc aaattttcaa agaccagacg ggaaaatagt ttatcagagc tgtactatag
    40261 aaaattctat tgcaacaact ataatagaaa ataacataac agaagttgcg ggggatgtaa
    40321 tagtagagtt tactttctac aaagatgatt tagttgttac tactttttct ttaaagatta
    40381 atatagagaa aagtatagat aaaaacagta ttacagaaga accgaaatgg gattatatca
    40441 gcgttacaat aaatcaagta aaagaagtcg tcgaaggcat agaagaaata aaagaaacag
    40501 aagaagcaag aaaagaagca gaaattaaaa g
//
LOCUS       DAELSY010000019        39788 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.019, whole genome shotgun sequence.
ACCESSION   DAELSY010000019 DAELSY010000000
VERSION     DAELSY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39788
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.019"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            276..854
                     /locus_tag="KPG09_000894"
     CDS             276..854
                     /locus_tag="KPG09_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG0965518.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKFVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            884..1030
                     /locus_tag="KPG09_000895"
     CDS             884..1030
                     /locus_tag="KPG09_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG0965519.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            1335..2714
                     /gene="scfB"
                     /locus_tag="KPG09_000896"
     CDS             1335..2714
                     /gene="scfB"
                     /locus_tag="KPG09_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBG0965520.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            2743..3240
                     /locus_tag="KPG09_000897"
     CDS             2743..3240
                     /locus_tag="KPG09_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBG0965521.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            3538..5202
                     /locus_tag="KPG09_000898"
     CDS             3538..5202
                     /locus_tag="KPG09_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBG0965522.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            5215..7665
                     /gene="recJ"
                     /locus_tag="KPG09_000899"
     CDS             5215..7665
                     /gene="recJ"
                     /locus_tag="KPG09_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBG0965523.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            7678..8190
                     /locus_tag="KPG09_000900"
     CDS             7678..8190
                     /locus_tag="KPG09_000900"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBG0965524.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            8264..10471
                     /locus_tag="KPG09_000901"
     CDS             8264..10471
                     /locus_tag="KPG09_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBG0965525.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            10496..10945
                     /locus_tag="KPG09_000902"
     CDS             10496..10945
                     /locus_tag="KPG09_000902"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBG0965526.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNASRPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            10960..11574
                     /locus_tag="KPG09_000903"
     CDS             10960..11574
                     /locus_tag="KPG09_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0965527.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVSDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            11665..13221
                     /locus_tag="KPG09_000904"
     CDS             11665..13221
                     /locus_tag="KPG09_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBG0965528.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNRLDSESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDKKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKETKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEELNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            13211..14473
                     /locus_tag="KPG09_000905"
     CDS             13211..14473
                     /locus_tag="KPG09_000905"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBG0965529.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            14547..16334
                     /gene="aspS"
                     /locus_tag="KPG09_000906"
     CDS             14547..16334
                     /gene="aspS"
                     /locus_tag="KPG09_000906"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBG0965530.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTKMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDIQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            16793..18025
                     /locus_tag="KPG09_000907"
     CDS             16793..18025
                     /locus_tag="KPG09_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG0965531.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            18039..18845
                     /locus_tag="KPG09_000908"
     CDS             18039..18845
                     /locus_tag="KPG09_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBG0965532.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKHGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(18957..19826)
                     /locus_tag="KPG09_000909"
     CDS             complement(18957..19826)
                     /locus_tag="KPG09_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0965533.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            20197..21624
                     /locus_tag="KPG09_000910"
     CDS             20197..21624
                     /locus_tag="KPG09_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBG0965534.1"
                     /translation="MKLTKSISSMLCLALVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFLNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEIFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(21700..21969)
                     /locus_tag="KPG09_000911"
     CDS             complement(21700..21969)
                     /locus_tag="KPG09_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965535.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGISINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(21972..23414)
                     /gene="nhaC"
                     /locus_tag="KPG09_000912"
     CDS             complement(21972..23414)
                     /gene="nhaC"
                     /locus_tag="KPG09_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG0965536.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAATKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDILNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(23439..24605)
                     /locus_tag="KPG09_000913"
     CDS             complement(23439..24605)
                     /locus_tag="KPG09_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0965537.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESVLVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIK"
     gene            complement(24830..26950)
                     /locus_tag="KPG09_000914"
     CDS             complement(24830..26950)
                     /locus_tag="KPG09_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0965538.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVVDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHIARKILSINANEMIDSFVGDCIKDINFQNILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERAADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLGK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            27183..27584
                     /locus_tag="KPG09_000915"
     CDS             27183..27584
                     /locus_tag="KPG09_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965539.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            27627..27920
                     /locus_tag="KPG09_000916"
     CDS             27627..27920
                     /locus_tag="KPG09_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBG0965540.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            27935..28375
                     /locus_tag="KPG09_000917"
     CDS             27935..28375
                     /locus_tag="KPG09_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBG0965541.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(28467..28940)
                     /locus_tag="KPG09_000918"
     CDS             complement(28467..28940)
                     /locus_tag="KPG09_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG0965542.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(28940..29728)
                     /locus_tag="KPG09_000919"
     CDS             complement(28940..29728)
                     /locus_tag="KPG09_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG0965543.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            30056..31300
                     /locus_tag="KPG09_000920"
     CDS             30056..31300
                     /locus_tag="KPG09_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG0965544.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGIHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLINKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            31623..32720
                     /locus_tag="KPG09_000921"
     CDS             31623..32720
                     /locus_tag="KPG09_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG0965545.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEITIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            32711..33409
                     /locus_tag="KPG09_000922"
     CDS             32711..33409
                     /locus_tag="KPG09_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0965546.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            33412..34476
                     /locus_tag="KPG09_000923"
     CDS             33412..34476
                     /locus_tag="KPG09_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG0965547.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            34659..35363
                     /locus_tag="KPG09_000924"
     CDS             34659..35363
                     /locus_tag="KPG09_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0965548.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            35356..36678
                     /locus_tag="KPG09_000925"
     CDS             35356..36678
                     /locus_tag="KPG09_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0965549.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            36865..38238
                     /locus_tag="KPG09_000926"
     CDS             36865..38238
                     /locus_tag="KPG09_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965550.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIVMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(38611..39549)
                     /gene="pdaA"
                     /locus_tag="KPG09_000927"
     CDS             complement(38611..39549)
                     /gene="pdaA"
                     /locus_tag="KPG09_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG0965551.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDGGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT        15187 a         4567 c         6768 g        13266 t
ORIGIN      
        1 tgtgaggtac tctttttata tgaaataaaa tatattttta gccttttaac aatggggaaa
       61 aggtttattt ctattttgac ataggctttt tatgcatata aagcttgata aaattgtttt
      121 attataattg ttttgttata taaataataa taagaaaatt atataggtct ttttttgacc
      181 agttttgcat ttttaacaac tagttttgta aagtatattg aattttctca aaaaagaatg
      241 tactctatat atgtaaaatt tttttggagg taaaaatgtt taaaagatat gctgaaaaaa
      301 tgacatctgt tttaatttgt aataatatga tagataataa tgaatctaaa gtctattctt
      361 atggatttga aatattaata gctttcatag ttaatataac caccatgctg tttataggtt
      421 ttttatttgg taaatttaca tatgtattat tttttttaat gtgttattgt ccaataagac
      481 aattttcagg agggtatcat gcagacaatt attttagatg cttattaacc tttattttta
      541 taattttatc tacaatctta attatagaaa atataaatat agacttattt aaaaatatta
      601 ttatgattat tgcaagtgtt agttgggttg gaatttgtgt tctgtgtcca atagaacata
      661 ggagtaaccc tataagtgat agggaaaagt ttgtttataa gaaaactgct atatttatat
      721 ctacagtagt gttattgata acaattttaa gtctttcttt gagtatattt gtagattatt
      781 tcacatatag cgcatttgcc atgttttgga tttttgtgat gttagtatta ggtaagttaa
      841 aagctaaagt ataagtaaaa tatgtatgag ggagagttaa gttatgaaaa agtttattgt
      901 aagatttatg aagtttgcta gctcattggc acttagcact gctattttgt cagctaatag
      961 tacctgtcct tggattattc atcagcctaa agtacccaaa gaaatcagca atctaaagaa
     1021 gacaaattaa atacaagcac ttatttatac aaaattatca ggaaaaaaga aattattata
     1081 ttttaataag attaaaacaa tataaaatca gatacaaaat ttattttatt tattttatag
     1141 gagcctaata cctatttggt aatatattag tgcgccaatt tagggggata tagtgtattt
     1201 aatgaaatga cttacggaga gtaagtcatt tttgtattgt atgtatttta tgtaaactta
     1261 tgatataatg aagggtaaag tttaattaaa ataataaaaa actttagtat agatagtaat
     1321 ttaggaggta gggtatgagt atgatacata aattttctat gaatggatat aatatcgttt
     1381 tagatgtgaa tggtggagct gttcatgtgt tagatgatgt tgcatacgat ttgttagatt
     1441 tttataaaga aaaaagtaaa gaagaaatat tagagatatt aaaatcaaaa tatcaagaag
     1501 aaaaaataaa tgaagcatat gaagaaatat taaatttgga aaaagaaggt cttttataca
     1561 cagaagatac atatcaatat catccaagct ttgtacatag agagcctgta gtaaaagctt
     1621 tatgtttaaa tgtagctcat gattgtaact taaaatgtaa atactgtttt gcagctcaag
     1681 gtgattttgg aggagaaaaa gaacttatga gttttgaagt tggtaaggca gcaattgact
     1741 atttgattgc taattcagga agtagaaaga acctagagat agatttcttt ggtggagagc
     1801 ctttgatgaa ctttgaagtt gtaaaacaac tggttgacta tggaagaagt gtagaaaaag
     1861 attataataa aaatataaga tttacaataa ctacaaatgg tgtactgtta aatgatgaga
     1921 ttatagatta tataaatgaa aatatgcata atgtagtttt aagtttagat ggaagaaaag
     1981 aagttaatga taatatgaga ccaactttaa atgataaagg aagttatgat attacattac
     2041 cacgatttaa gaaacttgtg gaaaaaagag caaaagataa gtattattat ataagaggta
     2101 cttttacaag agataattta gatttttcaa aagatgttat gcattttgca gatttaggat
     2161 ttaaattaac ctctgtagag ccagtcgttg gagatgagag taatccatat gcacttagag
     2221 aagaagattt acctaagata tttgaagagt acgaaaaatt tgctgtagag tatgcagata
     2281 gacaattaca aggagatgga tttaaatttt tccattttat gatagaccta aatcaaggtc
     2341 catgtgtaat aaagagaata actggatgtg gagctggaaa tgagtatctt tctgtaactc
     2401 caaatggaga tatctatcca tgtcatcaat ttgttggaaa tgaagaattt aaaatggcaa
     2461 atatatttga tgaagaaata gtacttccag aaaatttaaa aaatatgttt agagaagctc
     2521 atgtatatac taaagaagag tgtaaacaat gttggaataa gttttactgt tcaggtggat
     2581 gtcatgcaaa tgcgataaac tttaataatg acatatcaaa accatatgaa ttaggatgtg
     2641 aaatgcaaag aaaacgtaca gagtgctcta taatgataca agctaagtta atgttagaag
     2701 gagcaaccaa ctaaatttat attttaaagg agttgtttat atatgattag agattattta
     2761 gaagataaac ctttaattga cgaaagtgta tttgtggcaa aatcagcaga tgtaattgga
     2821 aatgttaaaa taggcaaaga ttcaagtata tggtacaatg cagtagttag gggtgatgaa
     2881 ggaccaataa ctattggaga aaacactaat atacaagatt gttctatagt gcatggagat
     2941 acagaaacta taattggtaa caatgtaaca gttgggcata ggtctatagt tcatggttgt
     3001 aaaattagtg ataatgtgtt aattggtatg ggttcaataa tattggataa tgctgaaata
     3061 ggagaatata ctctgatagg agcaggtaca ctaattacat ctaataagaa gtttccacca
     3121 ggtgttttga taatgggttc tcctggtaag gttgttagag agcttacaga agaagataaa
     3181 aaatatattg atgaatctta tgaatggtat ttagaggcag cacaaaatca aaaatattag
     3241 tttaaagagt ggttgtttca aaacatattt aaatctgttt tgaggcaacc actaatttat
     3301 tttattagat tttgttgata ttttagtttt caaaaagtat ttgattaaac cagatataaa
     3361 atgtcaaaaa taaaaattaa ctgagttaaa taattttaat aaaattggta gtatatgtga
     3421 taaaattaaa tattaaagag gatataggat attagaatat caattggtga aataatgtca
     3481 tatacgataa attaaggtgt aagtttttgg gtatacctat cttaggagtg aaaagatttg
     3541 agaataagtt atagaaattt aaagcgatat agagaaattg gatatgtttt aataaagtat
     3601 ggttttagtt ttattgtaga aagactaaat atagaaggaa ttgcctataa aataccacta
     3661 tttgacccac cagaagaaat aaaaaatatg acaactggtg agagaatgaa aagagtttta
     3721 gaagaactag gacctacata tataaaaata ggacagatat tgagtacaag aaaagacttg
     3781 ttagaccagg atataattga tgaaatatca aaactcagag atgatgtaga gaaatttgat
     3841 tcaaatatag ctattgatat atttaaagaa gaagtgggtt taagtataga agaaatattc
     3901 ttagaattta aagaagaacc aatagcagca gcttcaattg gtcaagttta tgaaggtgta
     3961 cttaaaacgg gagaagaagt aatcgtaaaa attcaaagac caaatataga gaaaataata
     4021 aaatctgatt tagagattct tagaactatt gcaaatactt taaaagattt aaaaaaggat
     4081 tttaatttag atttagtaca gatgattgaa gaatttcaaa ctcaattaat gagggaactt
     4141 gattatacat ttgaagccat aaatgcaact aaattctcta ggatatttaa aaatagtgat
     4201 gaagtatata tacctaaagt atactcagaa tataatacta agaaaatttt agtaatggag
     4261 aaagttaatg gtacaaagtt aagtgatgta gaaaaaataa ggagactagg ttataacaca
     4321 aaaactattg ttgaaatagg agttaggtca ttttttacac aggtattatc tcatggattt
     4381 tttcatgcag acccacatcc aggtaatata tttgtagttg ctaaaaataa gatagcatat
     4441 atagattttg gaatgattgg aattattgat aataaaactt taaaccaatt aaatgaaatt
     4501 gcattagcag gtgtagaaaa gaatgttgac aagataatat atctattgat agaaatggat
     4561 gcattaaatg gagaggctga tattaaggga cttagacagg atttacttta tctaatacat
     4621 tattactatg atatttctat agaaaaaata aatgtaacag atatattaaa tgaactattt
     4681 aggtttttta gacagtataa aatagtcatg cctgcacaat ttgtaactct tgcaaagaca
     4741 gttataacat tagaaggaac aagtagaact ttaaacacag atttttcttt tggttctatg
     4801 ggaaaagagt ttatgaaaca tcattataag agtaaattta accctaaaaa tgttgtttta
     4861 agttcaagac aaaatgtaga agaaatatta ctggacataa aaactattcc aaagcaatta
     4921 aaagcaattt taaaaaatat agaaagaaat aatataaaaa tgcaaattga agatgttaaa
     4981 atgacgaggt tagaaaattg tataatagag ctaacatctc aaatatcttt gagtttagtg
     5041 ttggcatcta ttatagtagg ttcttctctt ataattgcat ctccaaatat agaaaataat
     5101 atatggataa aatttactgc aatagcgggt ttctttatat cctttataat aggcctttgc
     5161 ttagttataa gaagtattag gtcaaaatac aaaaaagatt agggggaatt aaatttgaaa
     5221 aaatggatat tgaagtataa aggtaagata gaggaaagtg aatttagcaa aaaacttaat
     5281 atttccccgg aaatctgcca aattttaaaa aatagaggga tatgtactga aaaagattca
     5341 gaaatattta tgaatccatc tttagattat ttaagagacc cttttttaat gaaagatatg
     5401 caaaaagctg ttgatagaat aaagttagca gttgagaaga acgaaaaaat ttggatttac
     5461 ggagattatg atgttgatgg tgtatcatct acatccatct tgtgtttata ttttaagagt
     5521 ataggttaca atgttgatta ctatattcct aatagattag aagaagggta tggtataaat
     5581 gaggatgcta ttaagcttat aagctctagg ggatgtgact tgattataag tgttgactgt
     5641 ggaattacat cagtgtctga ggtcaataca gcgaatgatt tgggcataga tgttataatt
     5701 acagaccatc atgaatgtca aagtgaaatt ccatcagcct ttgcagtaat aaatccaaaa
     5761 caagaagatt gtaattatcc atttgattca ctatgtggat gtggtgtagc atttaaaatg
     5821 attcaagcct tgactccaaa agaagaattt aaaactagta tgtataatta tttggaaata
     5881 gtaacactag caactatttg tgatattgtt ccacttatag atgaaaatag aataattgta
     5941 aaaaatggtt taaaatcaat gaaagaaggc aaaaatattg gattaaggga gcttataaaa
     6001 gtttgtggtg tagaaagtga taagataggt tcttctcata taggttttgc aataggtcct
     6061 aggataaatg cttcaggtcg attgggatat tcttacttag gagtagagct atttactact
     6121 cagtcacaag aagaggcagt agaaatagct agtatattgg aagaaaaaaa taatgaaaga
     6181 cagatgatag aagctaagat gtatcatgaa gctgaagaaa tgctaaagtc taatagcaga
     6241 tacaatgatg acaaagtgtt ggttttagcc aaagagggtt ggcaacatgg aataatagga
     6301 attgtagcat caaagttaac tgaaaaatac tacaaaccaa caattcttct aggtatagaa
     6361 aatggggagg ctactggttc agcgaggtca ataaaaggat ttaatatatt tgaagctctc
     6421 ataaagtgca aagacttgat gactaagttt ggtggtcatg aacaagcagc agggttatcc
     6481 ttggattcag acaatgtaga aattttagca aatgaaataa ataaatttgc agattataat
     6541 ttaactgaag atgatatgat agagaatgta aatgtcgagt ttgaattaca agaaaatgta
     6601 ataaatttaa atcttgtaga agaattacat aagttagaac cctttggatt aaataatcct
     6661 aatcctagat ttatagttag aaattatatt ttaaaagatt tgaaagttat aggtaagaat
     6721 caacaacatc tgaaattaag tatagaaaaa gagaaaagct atgaatgtat aggatttaat
     6781 atgagtcatt taaaatcaat gtataaagta ggagacaaag tagatgtact attccaatta
     6841 gatgaaaata actatatggg aaataggaaa gtacaatttt tacttaaaga tatacgattg
     6901 gctcgaccta aaagtgcaag taatgacaaa ctttcattga aattaatgtc aaaaataata
     6961 ccaaaagata ctcaaagcct gtataatata tctgtgtctg attttgaatt atttgatgga
     7021 aatacagata taaatatatt tgattatttt gaaaaagata cactaataat atctaattct
     7081 ataaatggat tttatagagc aatgtcagat atatcactta tagatttgga ttttaacata
     7141 aattacaata ttattgaaga tgttagcaaa aatactgata aattagagct tatattttct
     7201 cctaatattg ataaaataga cttaaaaaga tataataata ttattcttta tgattattta
     7261 tacaataaag gcgaatattc gtatatatat gagaataaaa gagaagaaag tgaaattata
     7321 aaatattata ataaaacaga cctattatat ttaaagaatg tagttagtaa tatagttcca
     7381 tcaagagatg aatttataac aatttataaa caagcactta taaaaaaaga aattgatttg
     7441 gatatggtaa atataaggga aacttttaat gtaattccat taaagttttt tacgatatta
     7501 aatgtattta gagaattaaa tttacttgac tttaatttaa attatgaaaa aaattctgta
     7561 ctcataagaa tattgccaaa accacagaaa aaattagact taaatgagag tttaatactc
     7621 aataatctaa aaaatttaga aaaacaatac aatagcagtt attaggaggc gtttcccatg
     7681 gatttaaaaa actttataag gaatattgat gatttcccaa aaccaggcat agattttaaa
     7741 gatgtgacaa ctctatttaa agatggagat gcatttaaat atgcagtgga ttctatagtt
     7801 gaagaattga aagataaaga tgtagattta gtaatagggc cagaagcaag aggatttttg
     7861 atgggtacac ctgtagcata tgctttagga gtaggatttg ttccaattag aaaacctgga
     7921 aagttaccag gtgaagttga gagttatgag tatggattag agtatggtac agatacttta
     7981 gagatacata aggatgcaat taaaaagggt caaaaagtag caatagtaga tgatttattg
     8041 gctactggtg ggactatgga agccgctgcc aagcttgtag aaaaattagg tggagaagta
     8101 gtttctatgc aatttttgat tgagctaaaa ttcttaaatg ggagagaaaa gttgtctaac
     8161 tatgatgtta attctcttat aaaatactag tacttttata atagttggta taacaccaac
     8221 tattttcata tatattaaaa ttttatacaa aataggtggt tatatgcatg ataaagaatt
     8281 acaagaaata attgaaaaaa taaaagttta tgcacctaat gcaaattttg aaatggttga
     8341 aaaagcctat aatcttgcaa aaaaagcgca tgaaggacaa tatagaaaat ctggggaacc
     8401 atactttatt catccattag cagttgcaaa tattttaata gagatggaat tagatattga
     8461 aactataaca gcaggtttac ttcatgatgt tgtagaagat acagaatata cctatgaaga
     8521 cataaaaaaa gaatttggca aagaagtagc ggatttggtt gatggagtaa ctaaacttgg
     8581 tcaaataaaa tatcgttcga aagaagaaac tcaatctgaa aatttaagaa aaatgttttt
     8641 ggcaatggcg aaggatataa gggttatact tataaaactt gctgatagat tgcacaacat
     8701 gagaacatta aaatacatgc ctccagaaaa agcaaagtat aaagcaaaag agacacttga
     8761 aatttatggt ggtatagctc atagattagg aatttccaag ataaagtggg aactagaaga
     8821 cagagcatta agatttatgg acccagaagg gtattatgat ttagttagta gagtatcaat
     8881 gaagagaagt caaagagagg attatattca aggaattgtt gaacttctta aagataagtt
     8941 taaagattta agtataccat gtgatgtata tggtagacca aagcattttt acagtatata
     9001 tagaaagatg caaaaaaaac ataagacttt tgaagagatt tttgatttaa cagcagtaag
     9061 gatattggtt gataatgtaa aggattgcta tgcagtactt ggtatagttc atacattatg
     9121 gagacctatt ccaggaagat ttaaagatta tatagcaatg ccaaaaccca atatgtatca
     9181 atcattacac accactgttg taggtcctga tggagaacct ttagagatac aaataagaac
     9241 tcatgagatg cataatatag ctgaaaatgg tatagcagcc cattggaaat ataaagaagg
     9301 tatctctagc aaagagtcaa aggttgaaga aaaacttcaa tggttgagac agatgatgga
     9361 gtgggaaaaa gatttaaaag acccacaaga gtttatggat gcacttaaag aagatgtatt
     9421 taatagtcaa gtttatgtat ttacgccaaa aggagacgta attgaattac cagcagaatc
     9481 aacacctata gattttgcct atagagttca tacaaatgta ggtaataaat gtgtaggagc
     9541 taaaattgat ggaagaatag ttcctattga ttataagctt caaaatggta atattgtcga
     9601 ggtacttaca tcatctaatt ctaatggacc aagtagggat tggcttaata tagtgaagac
     9661 cccaaatgca aaaagtagaa ttagacaatt tttcaagaag gaaagaagag aagaaaatgt
     9721 tgaccgtgga aatacaatat tagaaagaga atttaagaag catgatatac cacaaaaaga
     9781 ccctcttgtt gaaaaatata tggcattagt tgctaagaaa tttaatcagc caagtgttga
     9841 ggacttaatt gccactgtag gttatggcgg tataatgtca tctcaagtgg tatctaaggt
     9901 aaaagaccta tatttaaaag aggttaaaaa agctaaaaag gaacaacaga tacaagaaga
     9961 agatttaaat aaacacaata taagtgaaac agaatacagt aaaaagagaa agaaaaacgc
    10021 atcacaagga attatagtaa aagggcttga caatatctta gtaagatttg ctaagtgttg
    10081 taatcctctt ccaggagatg aaattgtagg atatattact aagggtaggg gagtagccat
    10141 acacagagct gattgcccaa atgcaaacaa taatggtgat ttcttccaaa atagattggt
    10201 ggaggtttca tggagttcag caggaaaagg taagtttgaa gcggaaattc aaataaaagc
    10261 tattgacaga agaggaattg ttaatgaaat aactcatatt gttgcaatgg ataaacttag
    10321 tttaaacgga ataaatgcaa gaaaaggtaa ggatagtatt gtaagtataa acttacttgt
    10381 agaggttaat gacacagagg aattaaaaat tctaatgaaa aaattaaaag caattcctgg
    10441 tgtagaaagt atttatagaa tgacaaatta gagttaaaat aatagggagg aaactatgag
    10501 agcagtagtg caaagagtat cttcatctaa agttacagta gatgaaaaca ctataggtca
    10561 aataaataag gggttactag tattgttagg agtgactcat gatgataaat caagtgatgt
    10621 agattatatg attgataaaa tattgaatct gagaattttt gaagatgaaa atgacaagat
    10681 gaatttatct ctaatggata taggtggaga gcttttagta gtatctcaat ttactttata
    10741 tggagattgc agaaaaggaa gaagacctgg atttagtaat gcatctagac cagaacttgc
    10801 aaataacctt tatgaagaat ttgtaaaaaa agcaaaagat aaaggtgtga cagttggtac
    10861 aggtcaattt gcagctcata tgatggtaga gttaactaat gatggtccag taacaatact
    10921 attagactca agtaaatctt tttaaggggg aaaactttaa tgattataaa aaaatttgta
    10981 gatagttatt ttggagtaaa tacttatgta ttaggtgatg aaaagactaa aaaatgtgca
    11041 gttatagacc caggtggaag tttagtagag acattaagtt ttgtaaaaga aaatgaatta
    11101 aatattgaat atattatcct tactcatggg catggagacc atataggata tgttaaggat
    11161 attaaagaaa aaactggtgc taaagtagtt gcacatgttg atgaaaaaga attattaaat
    11221 gataaaaata aaaatctaag ttatacaatg agatgtggag cgcaagagtt tgatgcagat
    11281 atctatgtga gtgataaaga caagcttgaa cttggtgaat taaaaatgac tttcttgcat
    11341 actccaggac atactcaagg atgtatgtgt ataagagttg aagatgaaat gttcacagga
    11401 gatactttgt ttgctggaag tattggtaga actgatttat atagtggaga ctttaaccaa
    11461 atggaaaaat cacttaagaa attatgcaaa tatgaagatg ctgttagagt atatcctgga
    11521 catggaccaa ccagtactct aggggttgaa aagaagacaa atccttacat gtaattttat
    11581 attagtattt aaaaaagtat aaaatattta ataactatat tagtgagggg gttcttctac
    11641 tgttaaatag aaggagttaa gttgatgttg tatgtttttt taaaaggaca tgattataag
    11701 tatgaagtag cagaacttat aaaattgttt accagtgaat ttaagtttaa agactctaat
    11761 gctaacaata atatagagtc aaagcattta ataaatagat tgatttatga gaatggtgta
    11821 ttattctcaa gtactgaata ctatgaaaat ggaaatttaa agtacgagtc aatacagaat
    11881 atagaaaata tgaatcttga atttttagat ggatttgata gtagcaaaac gattgaagaa
    11941 tttcttaata gactagatag tgaaagtaag aggaacttta aaaagctgtg taaagaaaat
    12001 atcaaaaaaa gcatgtttgt agtgcttaaa caagtattta attcttatgt accatgggga
    12061 attttgactg gtattagacc agtcaaaatt gtccacaatt taatggataa aaagctagat
    12121 gataatagca taagaaccat tctaaaagat aattatttta taatagataa gaaaatagat
    12181 ttagctcttg aaatagctaa aagagagcga ttatttatgt atcctataga taaaaataaa
    12241 atttctcttt atgtaagtat tccattttgt ccaactagat gtgtatattg ttcatttcct
    12301 tcaaattcat taaaacaatt tggacattta aaaagagaat atgtttataa attgattgaa
    12361 gaaattaagg ggtttgccaa agttattaag gagactaaga aagaagttga aactctatat
    12421 attggtggtg gaactccaac tacacttgat gaggaagaat tagatttgct tataaattca
    12481 ttgttcaatg aattagattt aagtaaaatt aaagaattta ctgtagaagc aggtagacct
    12541 gacactatta cagaacaaaa actaagagtg ttaaaaaaac acaatgttag tagaattagt
    12601 ataaatcctc aaactatgaa tgatgatacc ttggtaaaga ttggaagaga gcatagagta
    12661 tctgatttag tagattgctt taatatggct cgtagaatgg gatttgataa tataaatatg
    12721 gatataattt taggtcttat agatgaagaa ttaaatatgg taaagaacac attggaggag
    12781 ataaaaaaac tttctcctga aagccttaca gttcatacat tagccataaa gagagcatcc
    12841 aacttaaata taaatatgga taggtataaa gaacatctaa cacaatatga agaaatggta
    12901 aaaatgatag atttatcaat gaaatatgca aaagatatgg gattaaatcc atattatatg
    12961 tacagacaaa aacatatgtt gggaaattta gaaaatatag gttatgctaa agaaggatat
    13021 gagtgcattt ataatataca gattatggaa gaaaaacaaa gtaactatgc attaggtgca
    13081 ggtgctatat cgaaatttgt ttatgtagat gaagatagaa ttgaaagagt agaaaatgta
    13141 aaaaatgttg aacaatacat tgaaagagta gatgaaatga taaaaaggaa gaaagaagag
    13201 gtatataaaa atgttgacta aagctcctag aggaactaaa gacataactc caaaagaggc
    13261 ttacaaatgg cgttatgtag aaaataaatt tagagaaata tgtgctttat atggatatga
    13321 agaaatggta acacctatat ttgaacatac agaacttttc aagagaagtg ttggagatac
    13381 tacagatata gttcaaaaag aaatgtattc ttttaaagat aaaggtgata gagaaattac
    13441 cttaaagcca gaaggtacag caggtgtagt aagagcattt atagaaaata aattatatgc
    13501 agatactcaa ccaacaaaat tattttatgt gacaccttgt tttagatatg aaagaccaca
    13561 agctggtaga caaagacaat ttcaccaatt tggaatagaa gcacttggaa gtgatactcc
    13621 ttcaatggat gctgaaataa tcgcactagc tgttcaattt tttaatgaag ttggattaaa
    13681 tgatttggta gtgagtataa attcagttgg ctgtccagtt tgtagaaaag agtacaatgc
    13741 tttattaaaa gagtatcttg actcaaaagc tgatatattg tgtgatactt gcaatgagag
    13801 aagagaaaaa aatccaatga gggttataga ctgtaaaaat cctacatgta aagaaaatat
    13861 caaagatata ccatttattg cagaccattt gtgtgatgat tgtaaatctc actttgataa
    13921 acttcaagaa tacttaaagg aaatgaatat aaactttgta attgataaaa caatagttag
    13981 aggcttagat tactatagaa aaactgcttt tgaaattata tctaatgata taggagctca
    14041 aagtactgta tgtggtggag gaagatatga tggtcttgtt gaacaattag gaggaccaaa
    14101 aggtataagt ggaattgggt ttggactagg aattgaaaga cttttactta cacttgaagg
    14161 taatggaatt gaaatagaaa accctcaatc tacggatata tttatagtaa ctataggtga
    14221 agaagcaaat acaagaagct ttaaattatt aaaagacttg agacaaaatc atataagtgc
    14281 agataaagac catatagaga gaagtgttaa agcacaattt aaatattcag ataaaataaa
    14341 ttcaaaattt acaatagtga ttggagatga tgagcttaaa aatgacactg cgacattaaa
    14401 aaatatgaaa acatcagagc aaactactgt aaaattaagt acattagttg aagaattaaa
    14461 gcaaaagctg taaaataaga aagtatgatg ttttgtgtat tagataaata tatagcatta
    14521 aaattaaata aaatggaggt agtatcttgg aaactttaaa gggtttaaag agaactcact
    14581 actgtggaga gttgagagaa aaaaacataa atgaagaagt cgtacttatg ggatgggtac
    14641 agaaaaaaag aaatttaggt ggtcttgtat ttgttgattt aagagataca agtggattat
    14701 gtcaaatagt ttttgataca gatgtagata aagaagcttt tgaaaaagct gaaaaattag
    14761 gggctgaatt tgtaatagct gtaaaaggaa aagtatgcga aagacaatct aaaaacccta
    14821 atatgccaac tggtgatata gaaatatttg caactgaact tagactttta aacaagtcag
    14881 aaactccgcc tatttatata aaagatgatg atgatgtttc tgaagcacta agattaaaat
    14941 atagatactt agatttaaga aaaccatcta tgcaaagaaa tttaaaatta agacataaag
    15001 taatgaatat tacaagaaat tacttatcaa acaatagatt ttgcgaaata gaaacaccat
    15061 ttttaatagc tccaacacca gaaggagcaa gagattatct agtaccaagt agagttaatc
    15121 caggaaaatt ttacgcatta ccacaatcac cacaattata taaacaatta ttgatggtaa
    15181 gtggtatgga taggtatttc caaatagtta aatgctttag agatgaagat ttaagagcag
    15241 atagacaacc agagtttaca caaatagact gtgaaatgtc ttttgtcgag caagaagatg
    15301 ttatgagtat gattgaaggt ttgcttgaag cgatatttaa agaagtttta gatgtagaat
    15361 tggcacttcc acttcctaag atgacttatg cagaagctat gtctaaatat ggttcagata
    15421 aaccagatac tagatttgga tatgagttaa cagatatatc tgatgttgtt tgtaactgtg
    15481 gatttaaagt gtttgctgat gctacacaac caggaaaaag tgttagaggt ataaacgtaa
    15541 aaggcaaagc tgacgacttt actagaaaac aaatatcatc actagaagaa catgctaaga
    15601 catatagagc aaaaggtctt gcttggatga aagtaggaca agaaggagta acttctccaa
    15661 tagcaaaatt ctttaatgaa gaagaaatga acgctatact tacaaaaatg aatgctgaag
    15721 taggagattt acttttattt gtagctgata aaaactcaat agtatttgat gctttaggac
    15781 aagttagact tgaagtagca aataggctta atctattgga taaaaatgta tataacttac
    15841 tatgggtaac tgagttccca gtttttgaag aagatgaaga aacaggcaca ttttcagcaa
    15901 tgcatcaccc atttacatca cctatggatg aagatttaga caagttagaa gaaggtgata
    15961 agtcatcttt aagagcgaaa gcttatgata ttgtattgaa tggttatgaa ataggtggag
    16021 gaagtgttag aatatcaaat tctgatattc aatcaagaat gtttaaagca cttggtttca
    16081 cagaggaaag agctaatgaa aagtttggtt atttattaga agcatttaaa tatggtactc
    16141 ctcctcatgc tggtctagct tttggtcttg atagacttgt tatgttactt gcaggaactg
    16201 ataatataag agaagttatt gcattcccta aaaatcaaaa tgctgtttgc cctatgacaa
    16261 atgctcctac attggcggaa gatgagcaat tagaagaatt aagtataaaa gttgatataa
    16321 aagataatga ataatatgta gttgcaagaa actaaaatgt aaaaagtctg gatataaagt
    16381 atatttatat tcagactttt tttattagaa ttattttata ttttttctct atatattttc
    16441 ataaattatc cttgtattct cagccaatat aattaaaata ctatgtttaa aaattttaca
    16501 taaacttgat tgttttagaa attacttctg taatgagtac acgattttaa ttaaagaata
    16561 tataacaaat ttgaaataag tctaatatat gacttaatag attaaattaa aataataaat
    16621 ttggactatt tatctattta taacttaaat tatatcaaat tgataacaaa ctagatatta
    16681 tacttttttg tagaatagaa ttacaattat ataaagaaaa attctattaa tagtgaaaaa
    16741 aaagtagaaa aaagtagaaa ttattctttt ataaagaaag ggagggataa tattgcaaga
    16801 gagagaagaa ttagttttaa atccagttcc aaaagaggaa agagttggat ggttagctcc
    16861 attgtttaat atgcttggtt ctaacatagc tatatctgaa ctaatggttg gaggaacttt
    16921 aattttagga atgactcttt ctaatatgat tattacatca attataggga acttaatatt
    16981 agttgcaatc attatgatac aaggttatat aggttacaaa gaagggttaa atacgtatgt
    17041 attagctaag ggggcatttg gagagatagg aggaaagtat cttatatctc tattacttgg
    17101 tataactagt tttggatggt ttggtgtaca agcaggggtt gcaggactat cagtacaaaa
    17161 gatattccca agtgtaaacc taacattagt tacagttata cttggattat ttatggttgt
    17221 ttttgcactg tatggcttta aagctatggc taaatttaat tacttagtta taccaccact
    17281 tatgatatta atggtgtggg gagtttttaa agctttttct acttatggag ttgaagctat
    17341 ttataattat actcctcaaa caactatgag tatggttgaa ggtcttaata ttgtagtagg
    17401 tcttgttata gttggtgcaa ttatatcacc tgaccagtta agatatacga gacgagtaaa
    17461 agatatttgg attatcagtt ttttaggtct agggattatt tcattattcc aacaagttgc
    17521 agctggtgta atgtctatgg gagcacctac ttgggatata acagaagtat tggctaattt
    17581 aggttttgga tgggtagcgt ttgttatttt aatacttgca tcttggtcaa caaacgtatc
    17641 aaatgcttat tctggtggat tagcattaaa aactattttt ccaaatgtaa aaagaaatgt
    17701 tttgacttta gttgcaggtc taataggtac aataatagca gctactggta taatatttaa
    17761 atttcagtca tttttatctt tcttgggaat cgcagttcct gcaatagcag gaattatgtg
    17821 gtgtgagtat tattttatac aaggaagaac ttataagcat agagaaggaa taaattggat
    17881 tgcagtaata agctggttaa taggttttgc agcgtcgtat tatagttcaa aaataaattt
    17941 cttaatacct ccgataaatg gaattgtagt gagtatggtt atttattata tattaatgaa
    18001 atgttttgga atcaaagata aataaagggg gcaattacat gggtcgtaag gtgaaaatag
    18061 gtataattca acaacacagt gtacttggag atgttaaaaa aaatatagaa aaagcagtag
    18121 agatgataga tgacttagga aaacatgggg cagatataat ttgtttacca gaactatttg
    18181 ctacaggata taatttagag tctttaggtg gagttaaaac attagaatta ataagagaac
    18241 ataataagta catagaggaa tcaatgtcag aagctgctaa aagaaataat gtatacctga
    18301 tatctccata tggaacctta gaaaaagggt ctacacatgt ttataattca gcagttatat
    18361 ttgatagaaa aggtaagatt atgggagaat actgtaaaaa tcatctgtgg tcattagaag
    18421 cagtatactt taaaggaggg gaaaaagtag aggtatatga tgctgatttt ggtagatttg
    18481 gtgtaatgat atgttatgat gcaggttttc cagaagtatc cagagaattg acattaaaag
    18541 gttctgaaat aatatttata ccatctgcat ggagaataca agatgaagat atgtgggatt
    18601 taaatgtatc tcaaagagct ttagagaaca ctgtatatac agtaggtgta aatttagtaa
    18661 gtaatgactc taatttaata ctatttggaa agagtaaaat atgcaatcct agaggtactg
    18721 taataactca attagataca tacagggaaa aatatgtatt atcagagatt gatttagatg
    18781 aaataaaaaa gtatagggaa gatataccct atttgaaaga cagaaaaagt agctataaca
    18841 tataattcta tactataata caagttcaat ataaaatgat aaaaagagga tgtatcaata
    18901 ttaagattaa atttattata ttttgatata tcctctttaa tattatcaac tttgatttaa
    18961 atatttttaa aatagttgat aagaaagttt ttgaacaaca acacagcttt agattcatag
    19021 ttttcgctaa accatgaaag atttaaatat cttttaaatt caatatcttt gatatgtaac
    19081 aacttaatct cagggtcttg agataatccc catgattttc cacatataaa tccaactcct
    19141 tgaagtgatt ttataagagc ataaattgta tatggtgaat cagactcaaa tgctattttg
    19201 ggtttgaaat tagctgattc gcacaggata tcaattacct ctctataatt ctcacccttc
    19261 gttataacta taaagttttc atctgaaact tcgcttagat atacttcatc ctttaatgat
    19321 aaaggatggt ttattgaaac tcctaatagt atttcttcgc atgttaaagt tatgctattt
    19381 tctgagttta gctttgtaaa tgaagaagat atgtacaaat caaaatcact ttttttataa
    19441 gatgatggca aagtatgtga cacattaaaa gtaatattgg gatatagttt tctgaaatta
    19501 ctcaaaagtg atggtaacac attagccgct gcaggagata atatctttac ttctcctgaa
    19561 ttttctaaag aagtgtcttg taactccttt tttgaacttt ctactaaatc taaaatgcta
    19621 tctactcttt ttagaaacaa tcttccatac tcatttaaaa caatatatct tccttttctg
    19681 tcaaataact gaactccaag ttctttttct aaggaagata tagttttgct taaagctggc
    19741 tgtgctatat ttaacttgtg tgatgctttt gttatgtgct ccattctagc aacagtttga
    19801 aaatacttaa gatgcaataa atccatagta taaaatacac ctcttttaat atgacttgag
    19861 ttatatcaat tttataatca actccacatt atacattata atataaagtt tatataatca
    19921 actacaatat attaaaaagt tatttaaatt gttatttata ttttgtacat aatctaatta
    19981 aaaaaagtag ctttaaatga ttaactaagg aaatgttgtt acaaacttta gttatataaa
    20041 aaattcattt gatgagtaaa gtattaatgt caagtaagaa taaaagctta ttagatagaa
    20101 aaataatatt ttataataat gatgttactt aaattattat gttatactat tttaaatagg
    20161 aaacatttag atactataat tttaggggga gtcaagatga aattaactaa atcaatatca
    20221 tcaatgctat gcttagctct agttttaagc ttatcaccag tagcatatgc aaatgaaata
    20281 agtagaacaa cagtggagaa tactttagta ggtcaggaca gataccaaac tgctgttgaa
    20341 gtaagcaaaa aaggatggag tagtgcgaat gaggctgtag ttgtaaatag tgaatctatt
    20401 gtagatgcat tagctgtaac accatttgct aaattaaaaa atgctcctat attattgaca
    20461 gaaaaaaata atctaaattc acaaactgag aaagaattaa aaagattagg agttaaaaaa
    20521 gtttatataa ttggtctatc tagttctgta tcaaatgatg tgcaaaaaca gttagaatct
    20581 aataatttaa ctactgatag agttggtggt agtgatagat accaaacagc tttgagtata
    20641 gctaaaaaaa tagaaggatt aaaagatata tcagaaatag ctgtagtaaa tgggtacaca
    20701 ggtcttgcag atgcagttag tatagcttct gtagctgcta ctaatggaat ggttatactt
    20761 ccagtatctg atgcatctgg tatatctagc tttaaagatt ttatagaatc taagaatata
    20821 aataaatcat acataatagg ctctactaat gctgtatcag ataaaataaa acaaagttta
    20881 cctaattcag aaagaatagg tggagcagat agaaatgaaa ctaatggtaa agtaatagaa
    20941 aagttttata catctaataa attaaacaat gtatttgtgg ctaaggatgg aatgaaaaat
    21001 caaaatcaac ttatagacgc tctagcagta ggagtactgg catcaaaaga aaattctcct
    21061 gtactaatcg taggagaaca attaagtgca acacaaaaga gtgtattttt gaataaaaat
    21121 gctacctcta taactcaagt tggtagtgga ggaaatgaaa atgcatttaa agaattaaaa
    21181 gatttacagg caaatagtaa caataagaga gtaatgtatg taactaatac agataaggta
    21241 aatataagaa atgatgctac aatagaagct tctgttatag gatatctaaa taatggtgat
    21301 gaagtagaag ttttagatgt gttaaaaact ggatgggtaa aaataaaata caatgaaggt
    21361 attggatatg ttagtgggtc ttatttgaca aataataagc ctgataatag taatgaaaat
    21421 ataaaaataa agtatgtaaa agaaaaagat ggtctaaatg ttaggaaagg accaagtaca
    21481 gaagatgaaa aaataggtca cctttcatat ggaagcaaag tagaaacaat agaaatattt
    21541 gctactggat gggtaaaaat aaagtataat ggtggatatg gttatgtaag taatgattac
    21601 ttatctgata caccagttat ttaattataa gtaacaataa aaaactgctt taatttgtaa
    21661 agcagttttt ttataaaaat ttaagtctaa taactatttc tattttgata ccttaatcct
    21721 gggatttatt attatggata ttatcaacat acaaacactc caaatccagt gtctaattcc
    21781 aaacttagat attaataaat tcagtcctgc tatggtaaat aagcagtatt taattacaat
    21841 taaaagtgta tttatagaaa tccccttcat agaaagtatc ctaaatccaa taatagacat
    21901 tctaagtcct ataaatgcca ataatatacc tgcaatatcc tgaattaagt aatagtattc
    21961 aaatttcata ttcaatcaac tcttctctga gcagtatcaa atatttctaa ctcttcaatt
    22021 tcatcatctg tgtatgtaag catagtaaaa ccagttacac cataaataaa tgaaattata
    22081 ggtgtaatcc aacttaagaa acaatatgga ataaattcta gtgggcttac accaagagtc
    22141 tgagcacaga aaacagcatt tgaattccat ggtattaatg gaccaccaag tgtagctgta
    22201 tcttctataa ttctagatag attttgtggt tttaatttaa actctttgta tattggagac
    22261 attagagttc cgacaaatac agaagtaaac atcattgatg aactaaatgc atttgcaaag
    22321 tagcttacta taaaagtaga cccaaccaat ttagttcttg attttcctat tttagttata
    22381 agaggttgaa gcagtacttc taagaatcca gttttttgga gaataccggc aataacgaaa
    22441 actacaaata ctagtaaaac ggtttcagcc atactcatta ttccaccacg atttaatagt
    22501 ttgtcggcat agacaaatcc agtatctata gtaaatcctg atagcatgta atttaaaata
    22561 tcacctattt tttctccttc ttgaaatact gctataataa gtcccatgat agctgaactt
    22621 agtatagata taatgggtgg cttttgtagc aataaaagta ataataaaaa tattattgga
    22681 atcattgcta ctaaaccaat atgaaagttt gaatttaata catccttgat ttgatttatt
    22741 tgaacatagt ctatagtatt atttgaatac ttaaatccaa ttactgtata cagaactata
    22801 catattacgt atgcaggtcc tgtggtataa aacatatgct tcatatgtgt aattacatct
    22861 gtcttacaaa ctgctgcagc taagtttgta gaatctgata gaggtgacat cttatctcca
    22921 aagaaagcac cagaaatgac tgcaccagct gttaaaccaa gaggaatacc catactagta
    22981 ccaatactca tcattgctat accagaagtt ccaagagtcc cccaagatgt accagtagca
    23041 acagaagtta atgaacatac gattaatgtt gtaagcagaa aatatttagg ggttattatt
    23101 gttagtccac tatatactac tgttggtatt gtaccagcag ctatccaagc tgctattagt
    23161 acaccaacag aaagtataat taagtttgat tgaaaggcat ctttaccaac ttcaaatcca
    23221 aaagcttcta tttcatcatt agtataacca agtttcgtgg ctgcaggaac tactataagc
    23281 catgaaagta aaaacattgt agttatagaa gcattaaata caactatacc aactgatgtt
    23341 agagctatta ttgcaagcat tactaacaag gaatatctaa atgaaggtat ttttctttct
    23401 gtattcattt tcatagtgca aattctcctt aaatattttt atttaattgc attttttatt
    23461 cttataagag cttcttcaag catacttctt ggacaagcta agtttattct ttgataccca
    23521 cttcctccaa tgccaaagct gtttccttga tttagagcaa ctttaccttt ttgtacaagt
    23581 acactttcta gttcctcatc acttaatcca agtgcactaa agtctaccca taataaataa
    23641 gtaccttctg gttttctaac tttaagtttt ggcatatttt catttatata ttttatagca
    23701 aaatctatat tagattctaa atattctaaa aatgattcta gccaagattc accattatta
    23761 tatgaagctt cagttgcaac taagctaaag caattatttc tctttatatc aattctggta
    23821 aaagcatcat ctaacaattt atagtctttt tcatcaggta gtattacata tgatgattga
    23881 agaccagcta tattaaaagt tttagttgga gccatacaag taattgtatt tttttcaaat
    23941 tctttagaaa tagatgccat aggaatatgc ttatgttttt ttaatataat gtcactatgt
    24001 atttcatcag atatgatttt tacattatgc ttaagacaaa tatctcctag tttttttaat
    24061 tcatcttttg tccatactct accaactgga ttatgaggat tacaaagtat gaataatttt
    24121 acatctttaa tcttgttttc aatgtcttca taatccatta tatagtttcc attttctagt
    24181 ttctgtaatg ggcttataat tagttctcta ttgttatttt ttacaacact attaaaaggg
    24241 ctgtaaactg gttcttgtat cattatttta tcatttgctt ttgtgagttc atttattaaa
    24301 agacttatag ctgggataac acctggacta taaattaacc actcactttt tattttccag
    24361 ttatgtctcc tatctagcca gtttactata gattcattgt aagaatctgg tcttgttgtg
    24421 tagccataaa tctcttgttc tagtctattt tttaaggaat caattataca aggtgcagcc
    24481 ttaaaatcca tgtcagccac ccacatagga agtaaatcat tagttccata ctttttttcc
    24541 atttctgacc atttagatga aaaattattg cttctatcaa caatctcatt aaaattataa
    24601 ttcattgtaa atacctccag taattttaat tttatgttaa taatattaag caaacaacgt
    24661 gccaatattg ataataaaaa atatttattc acaagttaat atttaatatt aagtaatttc
    24721 agagcataga taagataaaa atattaatta tacttaaaac tttgtggaaa aggttatttc
    24781 taataaattt ttaaatatat gaaaatgtaa ttaaataggt taaataggtt taactgttta
    24841 acttgttaga cctatttata ttttttagaa attctactcc ttttgatgtt ataattgaac
    24901 ctccacgacc aacaagtatg tctacaagcc caaagtcttg tagttctaaa agcatatttc
    24961 taatctgttg ttctgtcaaa aatctatttt cctctaaagc tattttacat aagctttttc
    25021 tacctattct ttcttttaaa tcataagcct tttttaaatt atccaatata aatatgtatt
    25081 catctagtga ccttttaaaa ttatagtcgt atttcatgat gtttttgtct ttgccaatgt
    25141 tgaattgata cttttttata ttatcactta ctttattaca aacggttcta ctataatttg
    25201 aatctatagt gtccaatata tattcaggta aatcacatat ttctataata tcttttccca
    25261 agtaacagaa gtattctcct aagtttctta attctcttac atttccttcc catctataca
    25321 ttttaaagat ttcttttact tcctcactta gaataaaatt acatggaata tcatatttta
    25381 atgaaccaaa aatttcaaat atatcagcag ctctttccct aagtggatgt attttaaggg
    25441 gaagaacatt caacctaaaa tataaatctc ttctaaacat atttttagat acaagttctt
    25501 ttaaatttct attagtagct gcaattattc ttacatcaat atcaatgaca ttattagaac
    25561 caatacgtcg tacttgtttt tcttgtatga ctctaagtaa tttagattga gaatttaaat
    25621 ccatttctcc aatttcgtct aaaaatatag ttccattgtt ggctaattca aataaaccaa
    25681 ttttcccacc ttttttagca ccagtaaaag ccccttcatc atagccaaat aattcacttt
    25741 gcagtagatt ttcttgaaaa gtactacaat ttactgcgac aaagggacca tcttttctcc
    25801 tagaagcact atgaatggat tgagcaaata attctttacc agtaccactt tcaccaatta
    25861 taaggataga tgaatttgat tgagccattt tattagcaat tttttttgta ttaatagttt
    25921 gtatactaga acctaatata tcatcaaatg tgtatttact gatattacca gagtttacta
    25981 gttgtgctct taatttagct tgcttttttt ctaattgaga aaactttgtc atctttagta
    26041 taaatccatc aaatacattt aattgtatat gtttaatctc taaatttata tcaatatgat
    26101 ttattttaat caatttctca aaaaatggag ttttattggt caatatgttt tgaaaattga
    26161 tgtcttttat acaatcacca acaaatgagt caatcatttc atttgcattt atagacaaaa
    26221 tttttctagc tatatgatta tagaattgaa ttattccatc attatttgtg catataattc
    26281 catcatcaat tatagatagt aacaaatcaa attgtctttc aaacttattt gcatctaaca
    26341 ataactcacc tgtactataa cttgtaggaa ttattttttc tacgtatttt tttattaggt
    26401 catcttttat tagatgtttt aatttggttt ttgtagcaat ttcaacaata caacttaaat
    26461 ctattattct gtattttaaa tctacaactt cacaattttt aaacttaggc aggatttttt
    26521 cgccaggagt taggattaca gcatctgtgg gagtaaactc aatctctgga tagcagggca
    26581 ggatatttat attgtctatt cctaatctat gtattaaaga aattgtttct attgccatat
    26641 ctttacttaa gttatataca taaataattt tatctttttc taatttggat atcatatcaa
    26701 tactgttctt ttcaaatgtc aacttgggaa caatgatttg agcatcatag tgagataatt
    26761 ttactatttc atcgtatttt aaataagcag ataggagtat aagtttttct ttaaaaaact
    26821 ttaaatttcc ctcttcaaat gagtataaat ttatctttag attatcacct aagaattgcc
    26881 ttatttgatt ttcataaatt tctccagcgt ctttttttaa acttacaata gctaattcca
    26941 tttgtttcat tttaacctct catacaaatt tgacttattt tagattacat aataattaaa
    27001 ttagattaaa taatattatt taattattat tataaattta ttatagacta cacattgaag
    27061 taaaagaagt agctaaaatt attcctttta tttttgtttt ttgtatctat ttggtataat
    27121 agtattagag cgtataataa tttaggcaaa attttttaag gaagactaga ggagaatata
    27181 aaatgaaaaa atgtctttac tgtaataggg atataaagtc accaaataat aaattagcta
    27241 taaaatatga tgacaatatg aatatatatc catgtcgtgc agaatgtaag gaaaaaattg
    27301 aagaatatat agcaaaaaaa cctacatata agtttataaa tatattagag gttcttttag
    27361 gtgtgggcgg aatattctgc tttttgagta aatggactat tgcagcccaa gttgtacttg
    27421 gaattgatgc actggttata tttttattcc cattggcagg atttaagatg aatggaaagt
    27481 taagtatgga acaaactaag aatcaaagta ggtctattgc aatatcaatt ttggcattta
    27541 tagtaataat aaccgtatta tattttaaac aagttggtaa gtagagcata attgtataag
    27601 acatcattag agtatgaggt gagtttatgg aagaaagaaa attagcacca aacaatgaga
    27661 gggaagctct cataaaaaga ttaaaaagaa tagaaggtca agttaagggg atacaaaaga
    27721 tggtggaaca agaacgttat tgtgttgata tattggttca aatatctgct ataaggtctg
    27781 ctataaatag agtaggcaca attatcttag agaatcatat aaaaggatgt gtagcagaaa
    27841 gcattaaaca tgatgatgct gataaaactg aagagatgat aaaagagctt atggatacta
    27901 taaataaatt tacaaaataa ggaggcggtt agttatgaat caacaaggat ttattatgga
    27961 aattgttgat gatagaactg caaagttaaa aatgcaaaga cattcagctt gtgctgcttg
    28021 tggaaaatgt gctactacaa attcagcaga gtcaaaagaa atattagtag aagtagacaa
    28081 cacaattggt gcaaaagttg gtgaccatgt agaagtgagt atggataata tgaatgtatt
    28141 aaaagcagct gtaatggctt atataatacc tcttatagca cttcttgtag gaacaatagg
    28201 gacttattat atattggggg caattggaat gacaactgga gttgaagcca taagtggatt
    28261 agttggaatt tgtcttacaa taatctgtta tttatactta aagaagaatg acaaaaaatt
    28321 tagagacagt agagaattta taccagtaat aacaagaata ttaattgacc tttaagataa
    28381 ttataattaa aaaatagcaa tgtgcttttt aaataaatac tcaatacgta tttgtagagt
    28441 ttaagtacat tgctattttt atatttttat tttgattttc tgtaaggaat aaagtttttt
    28501 ctgattttca aaagattcaa tgttcttaca aaagaagtta caactaatac accaagtgct
    28561 attgcagcac ttagaaatag cgcatctgta ccttttgaca tagccaaaga ataatttttc
    28621 tgaatcaaag acaacattgt attatatagc ccaattcctg gtattagagg aagaatacct
    28681 ggaattacga acacaatagc tggttgcttt agttttcttg ctaaagtctc actacacgct
    28741 gagaccaaag cagcagcaat aaaacctgca aaaactgcat ttgtagtaaa attaaataaa
    28801 taaacatata caatccaacc aattccgcca gttactccag ctggaagaag tagatatagt
    28861 ggagcattaa agaaaacagc aaatccagca gttgcaacac ctgaaaataa aaaatgcaaa
    28921 atcatagaca tatcagtcat tagaaaggac ctcctattct ataccatgta tcaaggacaa
    28981 aaccaactcc agaagcaatt gcaataataa tcatagcagc atcaaaagca cgagaaattc
    29041 ctgatattaa attccctgat attaaatctc taatagcttt tataaaagca actcctggga
    29101 gtagtggcat tatagcgccg actataagca ttttagggtc atctaaaatg cctatttcaa
    29161 ctagtagaac tcctgaaagt gcaattaaaa aagaagatac taaacaggaa aatgcagata
    29221 ttgcacttat cttcatcgtt ttatcataag taattgcagc aaagattgct attataaacg
    29281 taagtataaa atttaataca taattccctc caagcaaaca agcaaaacaa gcagatgcaa
    29341 ttcctgtaca agagtaaaat aaccataaag gataaggttt tacatcttgg atttcataaa
    29401 gacgtgcaat ggcattatca atatcatact ctttgtcact aacaaattct cgagaaaaac
    29461 tgtttaataa agaaatcttg tttaagttta tacctcttga ttgaatcgtt ttcataaacg
    29521 aaaagccatc aaatttctca tctgaaataa ttattactgt aggagttgta aaaatattta
    29581 catgataaaa ccctcttgac ctgcatattc taaggacact gtcttcaacc ctagatgttt
    29641 cagctccatt ttttatcatc agttccccta caaatagagc tagttttaga atatttttct
    29701 tatattcaag ttcctcattt tgattcataa atacacctcc aaattaaaaa acgtaatatt
    29761 gaaattatat caaattttta agtaatattc cttaatatct atataaatat attatctttt
    29821 ttttgtttca aaatagtcac atttaaatta tatccaatta aaagaattag acaatattat
    29881 ttattatatt ttaacttaaa aataaagaaa gtaactatgt taaatatgga aaaatggcaa
    29941 aacacttgac tattaattgg taaaggacta taataataaa ttatcatatt gataaaaacg
    30001 aaaattatga aatgtctgta aaaataaaat ttaaataata actaggagga gtaatatgtt
    30061 tgagaacttg aaaaaagcgg atcctgagat ttatgaaagt atgaaaagag agttaaaaag
    30121 acaacaaaga aatattgaat taatagcatc tgaaaacata gtatcagttc cagtaatgga
    30181 aactatggga agtcatctta caaataagta tgcagaaggt tactcaggca aaagatatta
    30241 tggtggatgt gaatacatag atgaagttga aactcttgca atagatagaa tcaaaaaaat
    30301 atttggagca gaacatgcta atgttcaacc tcactctgga gcaaatgcaa atattggtgt
    30361 atattttgct atgttaaaac caggtgatgt tgttatggga atgaatttat ctcaaggtgg
    30421 acatttaact catggtgctc cagttaatat ttctggtcaa tattataaat tttatgaata
    30481 tggtatagat aaagacagtg gacttataga ttttgatgaa gttagaaaga tagcacatga
    30541 agtaaaacct aaaatgatag ttgcaggagc aagtgcatat cctagagaaa tagattttaa
    30601 aaagtttaga gagatagctg atgaggttgg agcattgctt atggtagata tggctcatat
    30661 agcaggtctt gtggctgctg gtattcacca aaatccatgt gaagtggctg attttgttac
    30721 aacaacaact cacaaaactt taagaggacc tcgtgggggt gttattcttt gtaaagaaaa
    30781 atatgctaaa gacatagaca aagcaatatt ccctggtatt caaggtggac ctcttgaaca
    30841 tataattgct tctaaggcag tttgctttaa agaagcttta agtgatgagt ttaaagaata
    30901 tcaggtacaa gttgctaaaa atgcaaaagc cttagctgaa gaacttatta aaagagattt
    30961 taaattgatt tctggaggta cagataatca tttaatacta ttagatttga ttaataaaaa
    31021 tataactggt aaagcagcag aaaagagatt ggatgatgct tatataacag ctaataaaaa
    31081 tacaattcca tttgatccaa atggtgctct tgttacaagt ggaataagac ttggtactcc
    31141 agctgtaact actagaggta tgaaagaaga agatatggca ataatcgctg aggctataga
    31201 cctatgttta acttatgatg aagaatctaa ggctagaaca ttggttgttg gtttaactga
    31261 aaaatatcct ttatatgaag aatataatat tatggattaa ttataaagaa caactaaata
    31321 aaatacattt aaataattac aatgattgtg tatgtatttg aatatattta gatataaaaa
    31381 aggtgtctca aaatgaactt tttagtttat gaggcacttt tttgtatgga atcgaacata
    31441 tttgtgttat ttaaataata aaaaatatgg ttatctctat ttttagatgg tctctttgta
    31501 ttttttaatt aaaactttaa gatttcttaa gaattatact aggtttttct aaagatttaa
    31561 agtctatact tataagtata gtaatcatat gaaaagaaaa gctttaacga ggagggaatc
    31621 ttatgaaggt attaattctt acaggaaaat ttggtatggg tcattactca gcatcaaatt
    31681 ctttaagtga agatataaaa gcaaaatttg ataattcaga aataacaatt aaagatatat
    31741 ttgaatacat tatgcctaac tattcagata aaatgtacaa gactttttct attcttgtaa
    31801 atcggggaag tagtctttat aatttgtttt ataaatgtgc tgaaaatgga aagaaagata
    31861 taaaatttac tttttcagat tattttttaa ataaattaga tacattactt catgaagtac
    31921 aaccaacagt agttatttca acttttccat tttgttctca attagtatca agatataagg
    31981 aaaaatataa ctcaaatctt cctttaataa catgcataac tgatataagt agtcactcag
    32041 aatggattag taaaaataca gactgttatt tagtagctag taaatctact aaggaagaac
    32101 ttgtttttaa aggaatagat gaatctaaga ttaaagttaa tggtattcca gtaaaaaaag
    32161 aatttaaaag aatagaacat gtaaatcatt caactaaaaa aaatattctt ataatgggtg
    32221 gaggattagg attactgcct aaatcagaac aattttacaa agaattaaat agtttagaag
    32281 gagtaaaaac tactgtaata actggtaata ataaaaaaat gtactataaa ctatatggta
    32341 gatatgaaaa tatagaggta gtaggttaca ccaatgaagt atataaatat atgaaagatt
    32401 ctgaccttat tatttctaaa ccaggtggaa taacactatt tgaaacaatt tattcagaac
    32461 ttccaatatt ggcatttaat ccatttttac aacaagagat agataatgct agttttatat
    32521 taaataatga aattgggaga atacttggta aaaataaaaa atactatgta gatgagataa
    32581 aagatttaat ttatgatgat gctactctta aagaaatgag cagtaacatg aaagaattaa
    32641 aaaaacaatt tgacaataat accttagaga atattttatt ttcacttgat gaacaaggag
    32701 cgtgtagaga atgtatgtag taggtttaat tatcgttgtt gcattgattt ttttagtcca
    32761 ttcaattata cctacatact ataataaact attaaataaa gaggtattaa aaaatatggc
    32821 tggagaaaat gaaattgcac ttacatttga tgatggtcca gataagagat atacagaaaa
    32881 attactagat gtgttaaaag aaaatgatat acaagctatg ttttttgtag tagcaaagaa
    32941 tgctgaaaaa gagcctgaaa ttataaaaag aatgttgaga gaaaatcata tagttggttt
    33001 acattcattg gaacacagaa atgcatggct ttatagttat agttatgtta aaaaagattt
    33061 tattgaaagt acaaatataa tgaaaaattt aggtgtagat gtaaactact atcgtccacc
    33121 atggggtcat actaatatat tttcaaattc atttgttaaa aaatataatc taaaaatgac
    33181 tttgtgggat gtaatggcag aagattggga gaaagatagt acagtagaca ttattataaa
    33241 caagttaatg agtagaacaa aagaaaattc tataatttgt ttacatgatg caggtgaaaa
    33301 ttcaggtggt gcagtaggtg cacctgagcg tactattgaa gctttaaaga tagctattcc
    33361 aaaacttaaa gccagtggtt taaagttcgt aacaccagaa aggatgtagt tatgataagt
    33421 aaaactgaaa agaaaaataa atgcctaggt agtgtagctt ttttagtttt actaatgggc
    33481 ataacaggat attttgtttt tagagggcag tcagttgaat cattgataaa atcattaaaa
    33541 ggtgcaagcc ctatgtttat actaattgga tttgcaatga tgtttatata tgttgcctgt
    33601 gaaggtataa atatatactt aggtatgaaa gctttaaacc aaaaaactac tcttttaaaa
    33661 tgtatgggat atgcttttat tggattttat tttagttcta ttacgccttc agcatcaggt
    33721 ggtcaaccag cacaagttta ttatatgaaa aaagacgata taaatatatc atattcttca
    33781 cttatactat tagtcattgt tgttattcac caagtagtta tactggcata tagtggaata
    33841 atgttcatta tggaacgtga gtttatatta aataatgtaa gtggtatgaa cattttacta
    33901 atatatggtg taataacaaa tgttgctctt gtcattggag taattgcaat tatattttca
    33961 aaaaaacttg taaacaattt tataatatca ataacaaatt tattaggtaa gttaagaata
    34021 ataaaagatg ttgaaagttc aagaaaagtt ataatatctc aaattgaaga gtatgttaaa
    34081 ggtgctcaat atataaagca gaatccaaaa cttgtagtcc aaattttagt cataactatt
    34141 gtacaaataa cagctatgtt tttagttcct ttctttgtat acaaagcttt tcatctatca
    34201 acatatactg tatttgagat attagcgata caatccttgc ttaatattgc tgtttcttca
    34261 ttaccattgc caggggctgt tggagcttct gaaaatagtt ttatgacatt atttaagata
    34321 tttttccctg gtcacttatt agttccggca atgttactta gtaggggaat aagtttttat
    34381 gcatttgtag ctataagtgg attgatatgt atagtagtac atgtaaaatc ttctgaaaaa
    34441 gtaaaagcag tgaaaaaagt tgcttatgta agataatatt atatagatta tttttattaa
    34501 tatgtcaatt tgtgtgtaca ttttaattaa gttttaaaca tgtttacagg agtataaact
    34561 attaaaacgt gtgacattgc taagcttttg ctaataaagt ataatatttt atatagtttg
    34621 aaatatttaa gaaatatagc aaaaaaagga gcttggtgat gaataaagag attccaaaaa
    34681 aaattttagt agctgatgat aatcctgaga ttagggaaat cgtagaaata cttttaactg
    34741 gtgaaggtta tgaagttatt atggcaacga atggagaaga agctgtaaac ttagtagata
    34801 gtacgattga tttgattatt ttggatgttg taatgcctgt aaaaacagga tttgttgcat
    34861 gtaatgaaat tagagaaaaa acgacagcac caatattatt tttaactgca aaaactcaag
    34921 actctgataa agttattgga ttttcagcag gaggagatga ctatttatca aagccttttt
    34981 catattctga actaatttca agggttaagt cgctacttag acgttattat atataccagg
    35041 gaaaggaaaa ggaaagagag ccaaatatat taaaaacaaa agagttatgt gtcaatttag
    35101 atactcaaga tgttacacta aatggagaac ctgtgttttt gacaactata gaatatagta
    35161 tattaattct tatgttgaaa aatagaaaaa aagttttttc gtctgaaaat ttatatgaaa
    35221 ctatttggga tgagcagtat ttttatacgg caaataatac aataatggtt catattagaa
    35281 atttaagaaa aaagttagaa aaagaccaaa aaaatccaca atatataaaa accgcatggg
    35341 gaaaggggta ttatattgat taacaaaatt aagccatcta agttgggagt tatactattg
    35401 atatatgtag taatctcagc tgcagtggct ttcattttaa atataccatt ttactctgct
    35461 gttgaatcta tagtaaaaga tgtatcagaa gggaaagtat attttaaaag agaagtaaat
    35521 caacttatgg ataattttca aacttatgtg aataacaatg gtataagagc agatgatgga
    35581 gatgatatag caaaatggaa tagtgataat tggtttgttt ctatgaagat atataaagga
    35641 aataaggttt tttatgatac attatactat cctaatgaag tacctgataa gagtgaggaa
    35701 aatgagtttt atagcagtcc catggctaga aactatgaga tacaattttc tgatattaaa
    35761 gctaatatag ttgtagtagc tttatttaga tttagatttc aaaattttat agaagcaatt
    35821 tctattacat ttatggcact tacatttatt ataacatttt tactgttgtt tagtagaaag
    35881 ataaagtacc taaggaaaat agaaatgggt ataaagatag tagagagtgg cagtttaaag
    35941 tatagaatcc cagtaaaagg taatgatgaa ttatcatcac ttgcatctag tattaatgag
    36001 atgagtcata cattggagaa tcaacttata aaagaaaggg agatgaagaa taaaaataaa
    36061 gatatagtaa cttcgatatc acatgatgtt agaactccat taacatcagt aatttgttat
    36121 ttagatttga tcaaagacca taaatacaat agtcctgagc aattggaaca atacataaaa
    36181 aatgtaaggt ctaaagcata tcaaataaaa gatttaacag atgacctttt tacgcatttt
    36241 gttaattctg gagaggaagt ttcaaaaggg tatgaaaaaa taaatgctaa tgaatttatt
    36301 tttcaattac ttttagatag ttcatataca ttggaggaga aaggatttca tataacaata
    36361 gaaaatgaac tttataacca atatcatatt tatgtagatg ttacacaatt tagaaggata
    36421 tttgataatt tatgttctaa tataataaaa tatgctagga aagataagcc tatagtattt
    36481 ttaattaaag tagaggattc taaattacta attatacaaa aaaatagagt aagtaatttt
    36541 tctaagaatg tagaaagttt tggaattggt ataaagaatt gtgagaaaat agttgcatat
    36601 cataatggaa gcataaatgt aaattcagat gataaagaat ttgctattga aatatctatt
    36661 ccagcaaaag aatcataaaa atttaaatta tatattgtgt aattgctatt atatattgtg
    36721 taattgctta tgtaaaattc tatgaagcag gaagcatgtg actttagtta tgggatgttc
    36781 aatattgtgt acctctaggc aagtaacaag tttacttaca agaccatata atattattat
    36841 cttaaaaaaa ggggacataa taatatggtt ttgacaggag cacattatat ttacattcta
    36901 tttatggtaa ttatattaat tactatgatt atgaaaaagg atacaatagt accatgtata
    36961 ttaggtgttt tttttatggg attgttcttt gaaaaaaaca tctttggtgc tataacagca
    37021 gtgtttaact cttttattat atctttaaat gagttgggtc caattatctt gattatagct
    37081 attatggttg cattatctaa agccctagaa gccaataatg ctatacagta tatggtgaga
    37141 ccattttcga gagttataaa aaattctaat acagcatttt ttgtaactgg atttgttatg
    37201 ttagttcttt cttggttttt ctggccaact ccagctgttg ccttagttgg agctgtattt
    37261 ttacctgttg ctatgagagc tgggctacca gcaattggtg ttgcagtagc attaaattta
    37321 tttggtcatg gtcttgcctt gtctacagac tttgttattc aaggagctcc aagtataaca
    37381 gctggtgcag caggtgttgc agtatcatat gttataaatg atggtatgat tttgttttgg
    37441 gtgatgggaa tcgtaaccat atctatggca ttttatacat taaaaagaga tattaacaaa
    37501 ggaatgttta gagaagaact aaaaggtttt gaaagtgaag aagtagaaga gttcaatgga
    37561 aaatctaaaa tagcaactat tttagtagct ctgggatttc ttgctgatat aattgttatg
    37621 tatatatttg acctaaaagg aggagatgca tcagctcttt tgggtggaac agcagtattt
    37681 ttaataataa ttataaatgt tataaatttt ggcaaagata gtcttgaaaa tgtatgtgag
    37741 aatataattg atggttttgt gtttgggata aaaatatttg gagcaattat accaatagca
    37801 gcatttttct atatgggtga agttgcgccc ttgacaggtg tatttggaaa agtattagca
    37861 ccaggttcac aaggtttatt atcagatata ggcatagctc tttctcaaac aataccactt
    37921 aataaatttg ccgtatcagg tatagaaact gtagtggggg caataactgg gcttgatggt
    37981 tctggatttt ctgggatttc attagttggt tcacttgcct ctgtatttgg aactgctata
    38041 aatgcaagtg ttggtgcatt agctgcatta ggacaaatca gtggtatatg ggtaggtgga
    38101 ggctgtctag ttccttgggg tcttatttca gcagcggcaa tttgtggggt tagtccaata
    38161 gagttggcaa agcgaaactt tgtcccagtt ataaccggat tggttgtaac aaccatagta
    38221 gcaatgttta ttatttaata aatcgaataa atctttaatt gtacaaatta aaaatagatg
    38281 tgtagctata atttattata attgttttac taattttaag ccatacatct atttttattg
    38341 atatttttat tagtattctt taataaaata atatttttca ttatactata cataacatct
    38401 aaatataaca tctaaaatac tgttatctat gtttaatatc ttgccaaatt ttttgatgtt
    38461 ttattaccct gtcccaccaa catatataag ctatcgcaag ttttttacct atactatcac
    38521 caaacaaaaa ttttaatttt aatttattat tttataatct atattatatt aactatatta
    38581 gtttgtagac tttagatgta attctaaata ttaatactcc aagagttcaa attcatatcc
    38641 tagttttctt gcatttgata taaaatcatt taaaatttct gtactagttt tggaaactgc
    38701 atgtagtaac aagatagaac catcatgtag attatccatt attttttgcg ttgcttcttc
    38761 atgtgatggt tgtttatcag tatcccagtc tctgtatgca aaactccaaa atacagtctt
    38821 gtatcctaaa tttttagtca ttgccaagct tttttctgag tattttccca taggaggtct
    38881 aaaaaattta accatatctt tcccagttac atctttatat aacttttcaa catcatataa
    38941 ttcgtcatta aagtttttta gattgcttgt ttttgttggc atagatggat gggttttact
    39001 atgatttcca actatatgtc cctcagaaac cattcttttt actaaatctt tattttcttt
    39061 tatatatgga gctgtaacaa aaaaaactgc tttaacctgg ttttgtttta gagtgtcgag
    39121 tatctttgta gtgtatccat tttcataacc ttcatcaaat gtaagatata gtgttttttg
    39181 accatctttt gtaggtccat tgtatagggc atcatagtca gaaaatttaa attcaatgtc
    39241 cgtatttact tctggtgttt tatgcttatt gttaggtata taaaaccaat caagagtagt
    39301 gttatctaaa gaagatacat ctaatttatt attatcatta ttagtagtgg cttttgatgg
    39361 tttatctgtt ccgtctgaaa ctattacatt ggaagtatcc ttaccaccat ctattttttc
    39421 cttatcttct tgtaactgtg tttccttatt ttgattttcg ttttgattgt tttgtgaatt
    39481 tgaacatcca acaataaaaa aaatagaaat agaaagtagt gttaaataat gcaatacttt
    39541 tctaaacatt ttgaagctcc tttctaaatt aataataatt gcatatagta ttattataat
    39601 tggaaattac tattaataat agttatatct ttaaaaataa agtcacaata gtatgacaaa
    39661 gggtgaataa tcaataaatt taaaaaaaaa tgaaaaaaat agttgaccat attatgagaa
    39721 gatgttatta taataaatgt gctcaacaag aagtaacaaa ataacaagca caaagaagtt
    39781 aattgaat
//
LOCUS       DAELSY010000020        38825 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.020, whole genome shotgun sequence.
ACCESSION   DAELSY010000020 DAELSY010000000
VERSION     DAELSY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38825
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.020"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(658..3765)
                     /locus_tag="KPG09_000928"
     CDS             complement(658..3765)
                     /locus_tag="KPG09_000928"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBG0965552.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(3858..4090)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4265..4792)
                     /locus_tag="KPG09_000929"
     CDS             complement(4265..4792)
                     /locus_tag="KPG09_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBG0965553.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(4827..5612)
                     /locus_tag="KPG09_000930"
     CDS             complement(4827..5612)
                     /locus_tag="KPG09_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBG0965554.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(5622..5879)
                     /locus_tag="KPG09_000931"
     CDS             complement(5622..5879)
                     /locus_tag="KPG09_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBG0965555.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFNILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(5898..6356)
                     /locus_tag="KPG09_000932"
     CDS             complement(5898..6356)
                     /locus_tag="KPG09_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBG0965556.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(6396..7100)
                     /locus_tag="KPG09_000933"
     CDS             complement(6396..7100)
                     /locus_tag="KPG09_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBG0965557.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(7110..7745)
                     /locus_tag="KPG09_000934"
     CDS             complement(7110..7745)
                     /locus_tag="KPG09_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965558.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IPVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            8126..9166
                     /locus_tag="KPG09_000935"
     CDS             8126..9166
                     /locus_tag="KPG09_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0965559.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGIITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(9629..10984)
                     /locus_tag="KPG09_000936"
     CDS             complement(9629..10984)
                     /locus_tag="KPG09_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBG0965560.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPISTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(11010..11279)
                     /locus_tag="KPG09_000937"
     CDS             complement(11010..11279)
                     /locus_tag="KPG09_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG0965561.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(11569..13509)
                     /locus_tag="KPG09_000938"
     CDS             complement(11569..13509)
                     /locus_tag="KPG09_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965562.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCNMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAKDSGYNIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNQLDIDVEWDFTDYYEKLEKGENFDITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKTIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNSDEKQPNTPKDD
                     VESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNPITENDDNYDLSSKSVFGTNEH
                     SFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCILTPIMI
                     GSILIVNPDTRKSIIKFIKLKK"
     gene            complement(13693..15168)
                     /gene="spoIVA"
                     /locus_tag="KPG09_000939"
     CDS             complement(13693..15168)
                     /gene="spoIVA"
                     /locus_tag="KPG09_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBG0965563.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(15340..16635)
                     /locus_tag="KPG09_000940"
     CDS             complement(15340..16635)
                     /locus_tag="KPG09_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0965564.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNKSNENTTKVS"
     gene            complement(16819..18228)
                     /locus_tag="KPG09_000941"
     CDS             complement(16819..18228)
                     /locus_tag="KPG09_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0965565.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGR"
     gene            complement(18280..19419)
                     /locus_tag="KPG09_000942"
     CDS             complement(18280..19419)
                     /locus_tag="KPG09_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0965566.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            19738..20388
                     /locus_tag="KPG09_000943"
     CDS             19738..20388
                     /locus_tag="KPG09_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0965567.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSGDDELTMKMISSIEYIIKHAK"
     gene            complement(20545..21564)
                     /locus_tag="KPG09_000944"
     CDS             complement(20545..21564)
                     /locus_tag="KPG09_000944"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG0965568.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVINKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(21595..22209)
                     /gene="plsY"
                     /locus_tag="KPG09_000945"
     CDS             complement(21595..22209)
                     /gene="plsY"
                     /locus_tag="KPG09_000945"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBG0965569.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(22244..23563)
                     /gene="der"
                     /locus_tag="KPG09_000946"
     CDS             complement(22244..23563)
                     /gene="der"
                     /locus_tag="KPG09_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBG0965570.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(23597..24925)
                     /locus_tag="KPG09_000947"
     CDS             complement(23597..24925)
                     /locus_tag="KPG09_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBG0965571.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEIGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPENILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(25163..25363)
                     /locus_tag="KPG09_000948"
     CDS             complement(25163..25363)
                     /locus_tag="KPG09_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965572.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(25365..26297)
                     /locus_tag="KPG09_000949"
     CDS             complement(25365..26297)
                     /locus_tag="KPG09_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG0965573.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNM
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(26342..27274)
                     /locus_tag="KPG09_000950"
     CDS             complement(26342..27274)
                     /locus_tag="KPG09_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG0965574.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYDLSFFHCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNDVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(27329..28618)
                     /locus_tag="KPG09_000951"
     CDS             complement(27329..28618)
                     /locus_tag="KPG09_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0965575.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(28647..29339)
                     /locus_tag="KPG09_000952"
     CDS             complement(28647..29339)
                     /locus_tag="KPG09_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0965576.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(29354..30115)
                     /gene="pgeF"
                     /locus_tag="KPG09_000953"
     CDS             complement(29354..30115)
                     /gene="pgeF"
                     /locus_tag="KPG09_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBG0965577.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENITSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(30112..30573)
                     /gene="nrdR"
                     /locus_tag="KPG09_000954"
     CDS             complement(30112..30573)
                     /gene="nrdR"
                     /locus_tag="KPG09_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBG0965578.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIEEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(30595..30858)
                     /locus_tag="KPG09_000955"
     CDS             complement(30595..30858)
                     /locus_tag="KPG09_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG0965579.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(30962..31735)
                     /gene="sigG"
                     /locus_tag="KPG09_000956"
     CDS             complement(30962..31735)
                     /gene="sigG"
                     /locus_tag="KPG09_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBG0965580.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(31827..32549)
                     /gene="sigE"
                     /locus_tag="KPG09_000957"
     CDS             complement(31827..32549)
                     /gene="sigE"
                     /locus_tag="KPG09_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBG0965581.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(32574..33446)
                     /locus_tag="KPG09_000958"
     CDS             complement(32574..33446)
                     /locus_tag="KPG09_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBG0965582.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGSAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(33737..35011)
                     /locus_tag="KPG09_000959"
     CDS             complement(33737..35011)
                     /locus_tag="KPG09_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG0965583.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(35295..36452)
                     /gene="ftsZ"
                     /locus_tag="KPG09_000960"
     CDS             complement(35295..36452)
                     /gene="ftsZ"
                     /locus_tag="KPG09_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBG0965584.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(36642..36995)
                     /locus_tag="KPG09_000961"
     CDS             complement(36642..36995)
                     /locus_tag="KPG09_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBG0965585.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(37094..37768)
                     /locus_tag="KPG09_000962"
     CDS             complement(37094..37768)
                     /locus_tag="KPG09_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG0965586.1"
                     /translation="MKKKIVILGAFILGIFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(37773..38471)
                     /locus_tag="KPG09_000963"
     CDS             complement(37773..38471)
                     /locus_tag="KPG09_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG0965587.1"
                     /translation="MKKRFINKSIIIGSVVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKTEKENLQSEVNSVKKDIETYRGKNNKGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVFNTYMY
                     LKEDKLCMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(38494..>38825)
                     /locus_tag="KPG09_000964"
     CDS             complement(38494..>38825)
                     /locus_tag="KPG09_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBG0965588.1"
                     /translation="LIVDVDYSVDDNKSIKFKNYKTKENVFKTLNYLKEEGIYRKINY
                     VNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKILIDLQNKNTNGGTINLNYGKL
                     AVYSPEG"
BASE COUNT        11937 a         7124 c         4127 g        15637 t
ORIGIN      
        1 cttccactac caatagcttg tagtgtactt ccctcaagag gaatagtcgt tgaagtttct
       61 cctattcgga gtttacctgc tgattgccca ttttaaagta cttagggttt aaccttatac
      121 catctaacca attttttcta ctttcgtaac tttcacactt aggtttattt catccttatg
      181 ttttagtttg gttagcttta ggggtttcca gcaattcaag tagtattgga taggttctat
      241 cctatctcta catataggtt tccctatatg ctgactattt tagctactaa ctcatgacta
      301 aattcacgag tgtgcgtagc tattttttaa tcaatactta actttttgtt ttcaccatac
      361 atcaaaacgc acccctttgc ataataaata aaccaccctg cagaacacac gataaattta
      421 taaataaatt tatcagaagt agcttactaa aaatattatt catcctcact taaacgttta
      481 ttcattaata ttctatcaca tgacataaat aatttatatg atttatatag atttatttat
      541 tgtaaatttt ggaatattaa aacattttat tgaatagata attatattac ataaaaaatc
      601 cctatagaaa tctataaact aatttctata gggattttgg ttaactaact attcttttta
      661 tttttttata acttttatac ctgtcatatg gtcatttaag ttttgttcct ctaaagattt
      721 atcatctact ctgtttattt ctaaagctaa agtttcagac ttgatatatt cattaaaatg
      781 gtcaacagcc ttagcaattt catcatcacc acagtattct attactatat tatctaatac
      841 ttcaaagtta ttagattttc ttaattgctg tactttagat ataaattctc tagcataacc
      901 ttcattaatc aactcttcag ttagcttagt atctagtata acaaaaagat tgttttcaac
      961 agatacatta aatccttcct tagcagttat acctattaat actaaatcct tattaaattc
     1021 aaaagcttcg ccatctaaat caactgttaa agtctcacct ttttcaagtt tattagcagt
     1081 ttcatgagca tttaatttac ttaatgctcc aacgaataaa ttcatcttag aacctaataa
     1141 tggccctgct tccttaaagt ttggttttaa actaaagttc atatactcat ctaaatcctt
     1201 agcaaatatt acctctttaa cattaagctc ttcttttatt aaatcaacta catcacttat
     1261 tgtatcttca aacttaccat ctactaacac tttttgtaat ggttgacgaa cttttattct
     1321 ttctacttct cttgaagctc tacctaaagt aactaagttt ttagctaaat ccattttttc
     1381 ttctaattta gtatctacta actcttcatt acatttagga tattcagcta aatgaacaga
     1441 tacttctcct gttaagtttc tatacatttc ttctgacata tatggtgcaa atggagctat
     1501 agctctacaa agaccatgta acaattcata agttgtattg tacactgctt ttttatcttc
     1561 tgttaattca gttgcccaga atcttcttct tgaacgtctt atataccagt tagataaatc
     1621 atcatttata aaatcttgta tcatccttaa agttttattt aactcaaata tagctaaatt
     1681 ttcttctaca tctttcatta aattattgaa cttagataat atccatctat caagttctgg
     1741 tctatcttta tattctacaa aaaattcagt tggatttata tcatctgtgt ttgcatataa
     1801 agtaaagaag ttatatacat tcttcatagt acctataaat ttactttgga tttcttttaa
     1861 tccatccata tcaaatttag ttggagtcca tggtggagaa acatatagta aataccatct
     1921 taatgcatct gctccatatt ggtcaaacaa ttccattgga tttactgtat ttcctcttga
     1981 tttactcatt ttcttaccat ctttatcaag tacaaggtct ggaactaaaa cactcttata
     2041 tggtgctttt cctaatacaa atgttgatac tgcaagtaat gagtagaacc aacctctagt
     2101 ctggtctata ccttctgata tatagtcagc tgggaacaat tggtcaaaat tttccttatt
     2161 ttcaaatgga tagtggtgtt gagcaaatgg catagcccca ctatcaaacc aacagtctat
     2221 aacctctgtt actcttgtca ttggttttcc acatttttca cattttaagt gtatatcatc
     2281 tacatatggt ctatgaagtt caactgtttc tggatttaca tcttctatag ctttttcagc
     2341 taattcagct cttgaaccaa ctgaatctgt atgaccacat tcacatctcc atataggaag
     2401 tggagttccc caatatctag ttcttgatat tgcccagtca tttagatttt ctaaccagtt
     2461 tccaaatctc ttctctccta caaaacttgg gaaccatttt acaccattat tattttctat
     2521 taatttatct ttaagtttag tcatctctat ataccagctt ggttttgcat agtatactaa
     2581 tggagtttga catctccagc agtgtgggta attatgaact actttttctt tgctatataa
     2641 tttattttca gaagctagcc attttattat ttcaacatct agcccatctt ccattacaaa
     2701 tctaccttcc caaggagtag atgtaaattt accagtttca tctactggtt gaataactgg
     2761 taaatcatat cttttacctg tgttgtagtc atcttcacca aatgctggtg ctgtatgaac
     2821 tataccagtt ccatcttcag tagttacata atctcctact gtaataaaaa atgctttttt
     2881 gtcaacttct acaaaaggca ttaattgttc atattctaaa ccttctaggt ctttaccttt
     2941 catttcttct aaaacttcat aatcttcacc tagtacttta tcagctaaag gtttagatac
     3001 ataatatacc tcatcacctt ttttaacttt taaataatct acatcagcat ttactgttaa
     3061 tgaaacattt gatggtaaag tccatggagt tgttgtccat gctaagaagt attcatcagc
     3121 atcttttttc ttaaatttag ctataactgt attagttttt acttctttat atccttgtgc
     3181 aacctcatga ctagcaagac cagttccaca tcttgggcaa taaggaagta ttttatgccc
     3241 ttcatatata tatccttctt tgttgaattt gtttagtatc caccatacag actctatata
     3301 gttattatct aaagttatat atggattatc taaatcaact tcataagcca ttctctcagt
     3361 cattattctc cattgttttt caaatgagaa aactgactct ctacattttt ggttaaattc
     3421 agctattcca tattcttcta tttgttgctt gctagtaaga cctaactctt tctctacttg
     3481 tatttcaact ggtaatccat gtgtatccca accagctttt ctctttactt gataaccaga
     3541 cattgtttta tatctacaaa ctgaatcttt aagcgttcta gataaaacat ggtgtactcc
     3601 aggatttcca ttagctgttg gaggtccttc atagaatacg aaacttgggt catctttccc
     3661 tttttctaaa gttttttcaa gtatatttat gtctttccaa taatcaaaaa cttgtgcttc
     3721 tgcttgtttt acagaactgt ctaccaatgg cttaaatttc gccatgtaaa tcaccctttc
     3781 ctactaaata ttataattcc aaaatttttt attctttcac ataaatttaa atactaaaaa
     3841 actcgccccc tttaaaccaa agggacgagt tataaatcgc gttaccaccc taattctata
     3901 tgtaaactaa atactagcta catataacac tctaatagta taacggtact aaccggcaca
     3961 gcttagtaga atttaatcgt tcagcctgca gctcaagagt gatttttatc tttataaatt
     4021 cattggttct cagcataccc aattctctgt aaaattatct aaagataaat gtctctatca
     4081 acgcttttaa aaatttatct gtgtttatat gaaattttat aacaaataat ttgaatagtc
     4141 aatatatttt tgaaaaatat aataatttct atttttctta catttttatc ctatttttaa
     4201 tctttgttat agatttctca aactcacaca gttattctac ataaaaaata aacaaattac
     4261 aagcttattc taaagttgta gcagcttcat cattactata attatacact gttgttcctt
     4321 caaatgcatt tacacatttt tcatctacat cataaaaaat ctcatcaaca cttcttattt
     4381 catcttctaa cagtgattta aatttatttc taaatatctt aaattctttg actaaagcat
     4441 catactcttt tcttatctct atgacacggt tattagcttg ttctataatt tgtctagctt
     4501 ttaaatcagc ttcttcaaca attatcttag cctttttatt agccgcgcta caagtgtctt
     4561 ctgctgcact ttgagcagtt attaaagtag cttttagtgt ttcttcaata ttttcatact
     4621 tgtttacttg gtcttgatat aaccctaatt tttctttaag agctgtattt tccctacata
     4681 gagattcata atcttccttg atgatatcta aaaattcatc aacctcttca tctctatacc
     4741 ctcttaaacc ttttttaaac tctttatttt ctatctcaat tggagttagc attacatttc
     4801 ctcccagcta taatttaaat tagtccttat acaattaatc ttgcttgaac ttttattttt
     4861 ccttttttgg taatatctcc tatttgtgtt accttagctc ttcctttgcc tctaatagaa
     4921 atcaaatcat cattttttac tattttagaa gttgaagtta tcttttcata attgacatgg
     4981 actttttctc catttatgta ttttgcacta tcttgtctag atatattgta aataccactt
     5041 atcacacaat ctaacctatc tgaagatact gtaaatgata cttctttata ttttgaagaa
     5101 ctattaacaa tattttctct ggaaatttcc tttacaatta cattatttct tgaaaccttt
     5161 tctaagttta caattataaa atcgcaaata tcactactta caactacttg acaaaagcta
     5221 tcatgtataa ttatatctcc aattttttct ctttttatac ctagacccag tattgaccct
     5281 aagtaatctt tatgagatat atttttaaac ttgaaattcc cttctatttg taaaaatcta
     5341 agagtatctt ctatatcttc atattccatg taaaatggat aaataaatac agtgcttctt
     5401 tcagcttgtt cataacctcc atccacactg tattttattt catttgtaga atttaatatt
     5461 gctacagcat ttttcacttc atatggatta agaaactccg tagacttaac atcatagttt
     5521 tttatacaac tattagcctt gtctatcact ttaaacattt tatttttcaa gtctatatcc
     5581 tttatgtgat ttgttagttt taatttatcc atcctatcac cttagaatat aaccaatact
     5641 aattgtctta ggaacatcaa taatactatt gcaattaaag gagaaaaatc tataggtcca
     5701 tttgtatatt tatacattat agaccttatt ggcccttcta ttggctctgt aagattttcc
     5761 aaaatattaa aaattttact ttgtcttcca tttggaaacc aagtcatcaa gctttttata
     5821 actatcatcc atgctattat atctaaaaga taataaagtg caattcttat tgttcccata
     5881 tattaaccgc ctttttacta tttattttgc caagggaaga atgctttact ttctaattct
     5941 tttttcatat ttgcatctat atccacatta ttaggagcta gtataaatat tgatttagaa
     6001 accttttgta tacttccatc taatacataa actgccccat tcataaagtt aaatatagat
     6061 ttacgaactt cagaattagt caatccttcc aaattcacta taactgccct tctttgtttt
     6121 agatggtctg ctatagttgc agcttcatca taaaccttag gttcaactat tacaactttc
     6181 atttggctag ttgtatgtaa atttacaatt ttatttgctt ttgcagtact aaagccactg
     6241 ttcatttctt cttcttgaac tatatcgtcc atgccacttt catattcatc ttcaatataa
     6301 tcttcttctt catcaactat ccaattctta aatttagata ttattccatt tgacattatt
     6361 aacttcctcc ttaaaattta ttcaaatttg agtaattatt ttttattgta gtttctttct
     6421 ccaaagattg atgttccaac tctaacaatg gtagcccctt cctctatagc aatttcataa
     6481 tcgtgactca ttcccattga caaaacatcc atccctacat tagggatgtt tagttctttt
     6541 atttgttttg ataaatcttt aagaccttta aatacacctc ttatctcatc ttcatcttct
     6601 ataaaaggtg ccattgtcat tagacccttt atttttatat ttttatattt ttctgacact
     6661 tctttgacaa aatctacaac ttcttctcta tcaagtccat gcttagactc ttctttagat
     6721 atattcactt gtactaagca atctatttct ctgtttattt gttctgcacg tttttgaatt
     6781 tcttcgcata atgcaattct atctaaagag tgtatcatac aaactttatc gattatgtac
     6841 ttaactttat tggtttgcaa agttcctatc aggtgccagt ttaatttgtc acctataata
     6901 tcatactttc tagctaattc ttggggttta ttttcaccaa cagaagtgat tccttccctc
     6961 attgcctcat taactaagtc tacatcaaca gtctttgtga ctgccaaaag ggtaatctct
     7021 ttgctgtctc tatctacttt ttttgcagct ttatcaattt tgtttcttat ctcagacaaa
     7081 ttatctttaa tagaacccat tattatcacc tactttatcc ttatatttgt gtttatactg
     7141 ttaggtctta aaattatttt gtcatataga ttaacacttg taactccatt tatgtcattt
     7201 ttataataat ccacaaatac agaagattca ttctcataag ttattccttc taatatgaca
     7261 aattttacac tttgtgattg ttcatcaatt acatatacac cacgtttatt gtcttttgtt
     7321 tttatagaag ttttaggtat ttttaaacat tccatctgat tatatattat atcaaattct
     7381 tcgacacttg tatcctgatt tgcaacaatt tggttactta tttttaattt taccattatt
     7441 ttattattat tttcatatat tttgtaaact tttgcatcta atttttgctc atgatattca
     7501 attattacat tttgattttt ttcaaagtta ttagaatctt ctttactgat gtatgtagat
     7561 agataattac tttggtcatt ggcatctttt tctatagttc cttttacagg aatctttgca
     7621 tcaacttttt cattttttaa caccaacgta tctacatttt tgctaattat cattactaat
     7681 atattaaata ctaaatatat aagaatacca agtatcacta atctggtata ttttaccttt
     7741 ttcaaagact ctccccctat aaaattactt tcattataag tacttcttct aaaaaatgtt
     7801 tattcctttt ttataagcta atattttacg ttctaataat tattttttcc atattaaatt
     7861 tattttttat caatcttcct atacaagact gcattattta tatataattg tattataaag
     7921 tataagttta atttacattt tatacattaa gcttacttta tatgttttta tagttatttt
     7981 tatttttaga tgcaatataa aaccaattaa gatttaaaat aacttttgtt atgttgacat
     8041 ttatttaggt aatcatttac gaatcgggat aacgttatca taacaaaagt ttatgtccta
     8101 aaaaaaatac aaggaggatt ttcttatgtc agaaatttta ttaggaacag gtttactttt
     8161 attggttcta tgttgtttca caatttttag ttacaaggcc ccatatggta tgaaggctat
     8221 gggagctcta gcaaacgctg catgtgctac tttcttagta gaggcattca acctttcttt
     8281 acttggtgat gttttaggta tggaattttt tagaagtgtt ggagctgcta atggtagtct
     8341 tggtggagtt gctgctgcta tacttgtgcc tttagcctta ggtgtatctc cagtttatgc
     8401 agttttaata ggattatctt gtttagattt ttctatatta tctggattcg ttgctggata
     8461 ctttgtttct ttcttaatta agtttataga gaaaaaagtg ccacctggag tggatttagt
     8521 ggttgtaatt cttctatgtg caccgctatc tcgtcttata gcatcaaatg tagacccatt
     8581 agttaatagt acactactac aaattggaca agttttgata gaagctacca acattagccc
     8641 tataataatg ggaattatac taggaggcat aataacagta gttggaacgt ctccacttag
     8701 ttcaatggct cttacatcta taattggtct tactggtctt cctatggcaa taggagcatt
     8761 aggtgctttt ggttcagctt ttatgaactc aacactattt gcaaaattga aattaggttc
     8821 taaaaaagat attatatctg ttgctattga accattaacc caatcagaca taatttcagc
     8881 taatccaata cctatatatt ctactaattt ctttggagga gccttttctg gtatcattgt
     8941 agccttgatg ggtcttgtaa acatgacacc aggtacagct acacctgtgg ctggttttgc
     9001 tataatgttt gcttataatc cagttcaaca agttcttata acagctggtt tatgtgcttt
     9061 attcaatgta attgcaggat ttttaggttc aaaaatattc agtaccttta aagttcttac
     9121 tgctggtgaa atacgtggta ctgatgtaga agaagaaatt gcttaaaaaa caataagatt
     9181 tataaattta tatctcaagt agtagagttt tatattgtag gcaattttat taattgcaaa
     9241 cgttttattt tttatttatg tttatgatta ttatgtgtgt tgaatattta gataaatttt
     9301 tatttattat ttttatttag atttttgttt agttttttat ttggttgata tattaggaat
     9361 aaaaattagt atagtttatt attagttaga tagcataact ttttagatac ataaaaaatt
     9421 ttatattaat tagatattat taaaataaaa gcgatacatg aatttttaaa tgtccatgta
     9481 tcgctttatt tttgccataa tcttaacaac tgatttttaa agtttattat cttaacgaag
     9541 ttcattatct tatataatat ttaaatgcat agacttcttt aatttaacca tctaaatatt
     9601 taattaaata ttattatatg taatataact aatttttaaa tgaatgtata ggagctggaa
     9661 tccttcctcc tctttgagta aataattcag atgaatttgt acttataggc attactggtg
     9721 ctcgtcctaa aagtcctcca aattctacca tatcacctac atcacatcct ggggctggta
     9781 taattctaac ggctgtagtc ttattattaa tcatacctat tgctgcctca tcagctatca
     9841 tagctccaag cgtacctgct gtagtatttc ctggcacagc tatcatatca agacctacag
     9901 aacaaatagc agtcattgct tcaagcttat ctatacttaa agcaccttta ataacagcat
     9961 caatcatacc tgcatcttca gatactggta taaacgctcc actaagtcct ccaacatgtg
    10021 aacatgccat tactccacct tttttaacag catcatttaa aagtgctaat gctgctgtag
    10081 tcccatgagt accaacaact tcaagaccca tttcctctaa tatatttgct actgaatcac
    10141 caatagcagg tgtaggagct agtgataagt ctattatccc aaatggaaca tccaatctac
    10201 tagaagcttc ctttgccaca agttgtccaa ctcttgtaat tttaaatgca gtctttttaa
    10261 ctgtttctga aacaacatca aaaggctctc cctttacttt ctctaaagct ctcttaacaa
    10321 caccagggcc acttacacca acatttataa ttctatcagc ctcaccaaca ccatggaaag
    10381 ctcctgccat aaatggatta tcttctactg cattacaaaa tactactaat tttgcacaac
    10441 caaaaccttt tgcatctttt gttaattctg cagtttcttt tatgatttct cccatttctt
    10501 tcactgcatc catatttata ccacatcttg tagaacctac atttactgaa gcacatactt
    10561 tattagtact tgctagagct tttggaattg acttaattag tatcttatca ccttttgtat
    10621 atcccttttg aactaatgct gagaatccac caataaaatc tatacctaaa tcctctgcag
    10681 ccttatccaa tgtctgtgca aactttacat agtcattttc atctgttgca cctgcaataa
    10741 tggaaattgg agttactgat acccttttat ttacaattgg gataccaaat tcacgctcta
    10801 tatcttttcc aaccttcacc aaatctttag cagaactcat aatcttattg tatatttttt
    10861 ctctagcttt atctccatca gggtctatac aatctaataa agaaatcccc atagttatag
    10921 ttcttatatc caactgttct tcatctatca tctttattgt ttcaagtata tttcctacat
    10981 tcactattat cacctcccct tactgtctac tacagattat gcattgaatt aaatatttct
    11041 tctcgctgaa tttttacatc cactgataat ttcttacctc tttcaaccaa gtcctcaacc
    11101 gttttctcaa aagaatttaa tgatttctct aaatcaacta tcataatcat agtaaaataa
    11161 tcctgtaata ttttttgtga tatatctact atgttgatat tttgcttgta taattcatca
    11221 ctaattcctg ccactatacc tggtttatcc tttccaatta ctgttaaaat tgctttcata
    11281 ataatatcct cccttatttt tttataaatc ttatattttt acaaattgaa ctatgtatta
    11341 acatatattt catttatttg ttagtaaacc actttttatg tatatattta atatatttta
    11401 atacctatac attcatttgt caattttttt tcaatattcc attattttta tactatttag
    11461 aaaattcata ttttatatat gttttctcaa caataaatac agttttcata ataatgaaag
    11521 gtattactta gcaatctcgt gattttctaa ataatacctc tctaaaaatt atttttttaa
    11581 ttttatgaat ttaattatac tttttctagt atcaggattt acaattaata tagaaccaat
    11641 cataattggt gttagtatac aaaataaaaa aataatatct ttttttagat tatattcttt
    11701 agattctaat ttataattat tatttattat tccatcataa tcacttatat ttgacttatt
    11761 tttatttgta tcttgatttt tgttagaaat actttcccta taaggaaaag aatgttcatt
    11821 ggtaccaaat actgattttg agcttaaatc ataattatca tcattttctg taattggatt
    11881 tttattgtaa aatccttttt ctttatcaga attattttct ttattaccgt ctttaggtat
    11941 atcatgagga tttttatttt cattgctatc agtacttgat tctacatcat ctttaggagt
    12001 atttggttgt ttctcatcag aattattatt ttcattatta ttgttttgat tgttattact
    12061 atcattattt tcatttccat tattcccatc ttgattgtta tcatcctcat ctctatccgg
    12121 gtcgtctcta cctcctcctc cacggtcacc atcaatatca tcattttccc gttcatcatc
    12181 tggcgtctct tggttgtctc cgcctcccat tataaatcct ggtttaaaaa ctatatttga
    12241 atacccttct tttaatcctc ctaccacttt tactctaaaa caacgcaact taaaatcttc
    12301 aaaaaaaagt atagcttgat ctctaatatc ctcctgttct tcagatatcc aattgattcc
    12361 atcgcttgaa tattcaacaa aaaccttact tgcactatac ggcatatcat aaaataaaac
    12421 agctacatat ccattttttg tattttgaaa ttccagttcc aaattgtcta ttgttttttt
    12481 ctctataaca gatacattca aaataggaac tacatcatca tttattataa ttttttcttt
    12541 atttaacatt tctgttttaa actttcctgt tatatcaaaa ttttctccct tttccaactt
    12601 ttcataataa tcagtaaaat cccattctac atctatatca agttgattac cactagatgt
    12661 ttctaatacc atatatttgg gaaattttat attttctatt ttatcatctt tacttacttt
    12721 tatttcatca ctaaatacac caattcccct atgacaacat ttcacaatat tatagccaga
    12781 atctttagct atagagggta taagctcatt ataatcatca attatataaa ctttcttact
    12841 ttctgatata attgattgtg ccaacttatt attatctctt agtaacccac tttcattgtt
    12901 gctactatat gcttttattg aagtttcttc tatctcaaca tcacctttag aataaacacc
    12961 tatagcatgt tctccattca cttcaatatc cttatttagt attctaatat cattttcagt
    13021 ataaattcct attgcatctt ttccatttgc ctttattttt tcaacagatt cgtaggatga
    13081 tttaatatgt aagtctcctc cttcttcaac gtataaggct gtcccatcag tagcaattat
    13141 gttgtttata ccaatactta tacttccacc attttctata tgtattactc cttcttttcc
    13201 tccttcacct attatattca attcattatc atcaataata aatctcccat gattttttat
    13261 caatatttta tatttgttgg tatctattgt aaaattactg tcataaggaa tataaaatct
    13321 aaactcttca tctattacca tattacaaga aagagtataa actatttctt ctttattatt
    13381 tttattttct tgaacccacc tttcaaaatc ttcaagacaa ttttcctctt gtccaaaaac
    13441 tttatgtgga aaacttataa aacacaaaac tattatactc ataaaaataa atatctcata
    13501 ctttttcatc ttacactcct agaaattttt tagctttgaa acaatcctgt cgaaacatat
    13561 acttcaccca ctataatttt tccacaatta tcaattattg tcaatttata taagtatctt
    13621 tattaattgt tataaataaa aagctactta ctaaagtaaa cagacgtttc actttaataa
    13681 gtagctatat ttttataaca aaatagttat aatattagaa cttccttcat ttacaatttt
    13741 ttgtaaagtc ttttgcattt taactcttat ctcttctggc atagtatata atttactttg
    13801 taattgctct tttactaagt catgtagtga cttaccaaac atatttgatt cccataaatc
    13861 tgctggttgc tcttcaaata cttccataag atattttatc atctcttcac cttgattttg
    13921 atttccaact attggagaga cctctgtaga tatatctgct tttataatat gaagtgatgg
    13981 tgcattggct tttaacttga taccatattg ttttccttgt ttcattattt ctggttcttc
    14041 cagactaagt tcttctaaag aaggagctac tactccatat ccttttatct tggcatctat
    14101 taaagcactt tctattttat catactcatt tttaacttta gatagtctag ttatcagact
    14161 aagcagttga taatctcctt caattttaaa tccacttttt tcttcaagga cattatagaa
    14221 taaatcttgc tttgtctgta aatcaatatt tataacacct tctcctagct ctacattatc
    14281 aacacctgta tcttccaaaa actctaactc agaaaatcct tgtataatac cttctatatc
    14341 tcttatttta cctatatcta taatactttg ttttaatgtt gttattatgc tactttttat
    14401 ccaatgattt ctttcaagac cttcaaccca ttttggtaag ttgattctta tttctgtaag
    14461 tggaaaatca tataatacag cttccataac ctcctctata tcttcttctt ccatttcaac
    14521 cacattcatt ggaagaactg gaacttcata cttttcttcc aattcacttc taagcatact
    14581 cgtttcctca ctttttggac ttaacgtatt taaaaccact gcaaatggtt ttttaagatt
    14641 tttcaattct tgtataactc tctcttctgg ttctacatag ctttttctgt caattccagt
    14701 aactgaacca tctgtcaaga caactatccc tattgtagaa tggtctttta taactttttt
    14761 agttcctatc tctgctgcct tttcaaatgt catagcttct tgtgaccaag gagtggatac
    14821 taatctttgt tttccacctt cctcatgccc taaagctcct tcaactatgt atccaacaca
    14881 atctaccatt cttactttta aagatactgt gtcttttatt tttatttcta caccatctgc
    14941 tggtacaaat tttggttcta ctgtcattat cgttttacct gaaccacttt ggggtatctc
    15001 atctcttgtt ctatcctttt taaattcatt gtctatatta ggtataacca acttttccat
    15061 aaattttctt ataaaagttg attttcctgt tcttacaggt ccaactactc caatatatat
    15121 atccccctga gtccttttgg atatatcttc gtatatgtta ttattcatat tattcctcct
    15181 atacatctaa tcttattaaa ttatatttag agttaagggt taatattctt aaaatagaaa
    15241 aataaagacc agcaatatta atttaattta catatagaaa tttgtaaatt tcaatgtaaa
    15301 ttaaattaat attgctggtc caaatagtag tgtattatat taactgactt tagtagtatt
    15361 ttcatttgac ttattattca tctcaataag ttctttacgt ctttcttttg tcatgttcat
    15421 ccatataaat gtaaacacca atccaattat ggctactata gtaagaacta tgaagtagta
    15481 cctatatcca gttacacccg gaaatgcatc caatatcttc cctgctgcta aaggacaaag
    15541 tacttctgga agatatccta atgtagcaac tattcctata gcagttcctg atattgataa
    15601 tgaataatta ccttcttcaa gtaacgaata gtgtaaacct tgaattgcat acatagatac
    15661 atatattgca acacatccaa tcaatagtat ataaatcata gatgattttg ttggtgtaaa
    15721 tattacacct aaaagacctg caatcataag tataaatcca taagatataa tctttgatga
    15781 acctacttta tctccaagta atccagaagc agctgatgct actggtctac aatactgagc
    15841 taatattgag attgcagatg caaacaccac agtagctcca aatgcttcag ttgaataagg
    15901 cgtaaaataa taaaatgaca tattagctga atatgaacaa aacattatta ttgttattat
    15961 ccatgtatga ggcattttta ttactgcaaa aaaggcttct ttatcaaatt ttgctgatac
    16021 ctctttttca ttatccttta atttgaaaaa tactaatact ccaactatta catttattgc
    16081 cgagtataat actataatag ctgtaagacc agatttatca cttacttttg atgaaatata
    16141 cccaaatagt atcaatacta gagatagatg aattgcattg gtaattccac gaccactttc
    16201 cataaatcca aaagcttttc cttgttcatt ttcagatgct aacattctaa cagcttttac
    16261 aagagctggc caaaaagtaa gtattgttgt tattccccat aaggcatgga ttaaaaacac
    16321 aacaatatat ggaggataaa gtgttaatac aagaccagat attcctgtaa taattaaaga
    16381 acctgaaagc aattttctgg ctgaaatacg gtctgctaaa aaaccaccaa aaaggtatga
    16441 taccattgca agtagaccat aaatgcttcc taagcttccc atttgtgtat ttgtaagatt
    16501 aaatgcttgt gcaaaagtat cataataata attctttaaa tatggtaaag catatataaa
    16561 tgctcctgct acaaagagta ttatcaagac tttgaaattt ttccaaatgc ttggtttatt
    16621 tacttcaact gacatacttc ctccttatgt aaatacctgt aaattttcat gataattaat
    16681 tattgcttat tttattgttt attcaatatt tcggaatatt tattaatata ttaggttaat
    16741 ctatcaataa tttatggtga gttatgagct aaaagtaaac tcttttagct caccataata
    16801 attatatttt tatttatact aacgtccaga gttattagat atcttttttt catagttctt
    16861 ccatagcatt aaacctgtta ttataacaaa aagacctcct cctatcattt caatctcata
    16921 gattatatta tcttttaaag tctccataac tggtgccgtt gagaaaagta tgccaactac
    16981 agttactaca agtaccattc tggcaatatt gatacaaact gaatcacttt ttgcaattga
    17041 atatggtctt tcttcatttg gtcttgtctt tctaagtcta atataagatg taaaaagcaa
    17101 gacatatgga aataatgctg ttaatgctgt catagtaaca agaacattgt atatattatc
    17161 tactgatggt aataagtttg ttcctacaag aagtagactc actattatag cctgaactat
    17221 tactgcattt gatggtatat tgtatttatt tgtaacagtc aattgcttag gaaatacacc
    17281 ttctcttaca tttccaaaaa gcatctttac tggacctgct atataaagta ttaatgctcc
    17341 tagtactgat aaagttattc caaatgctac tattcttata aaccaacttc ctatacctaa
    17401 atcttgtgca actttagcta atgcatctag tatccctgta gacgctgcaa ttttatctgg
    17461 aggaagtatc attgttattg ctactgaacc taagatatag attcctccta ttaaaacagc
    17521 cacagttaat atagctttag gaaagttttt cttagcatta tccatttcgg ttatgaaatt
    17581 agcagtcgtt tcagctcctg ataatgcaaa tattatagca gatatagaaa ctaatgaatt
    17641 ggcatctatt tttggtatta tattttggat tgtatatatc gatgctgatg gagctttttt
    17701 gagaataact acagacataa atgccatcac tataagtaat atagcaggaa ttgtagaacc
    17761 taatgctcct gtattcgtaa aaaattttcc aaatgccatc ccacgagtac ttactaagga
    17821 caacagccaa aatattatga gtgaaagtgc taaaataaac atcttattgt ttgataaatc
    17881 tggatttcca agcatatatg caatatttat agctaaaaat gtcaagaaag aagaatacca
    17941 aaatatcttt gatgtccaat ttagccatga caccataaat ccccacttct ctccataggc
    18001 ttgtttaacc cattctccaa gtccaccatc ttttggatat gttgcagcca actctgcaca
    18061 tataagtgat gcaggaacaa agaatataaa agcaaacaat aaccaaactg gtatagaacc
    18121 aagacccaat ccaaaactag aggcagtaga tttagctatc catctaatcc caaatagtgc
    18181 tgatatattc ataaatacta aatcccaaaa tccaattttc tttttcactg taattcctcc
    18241 aatttcaaat ttattcaaac tcatacacaa tgtattgatt taaatatcaa gtataagttc
    18301 ttcaacactt cttggataat tagttataat tgtagcacca ttcttaccta ctattgctgt
    18361 atctgagtgt ctaaatcctc caactccagg tatatatatc cctggctcca tagaaaatac
    18421 cattccttct tctaatatta attcattgtc aaatcttaaa tatggctctt catgttctga
    18481 taaacctaaa ccatgaccaa ttctatgatt tgaatataac tctagaccat actttgcaac
    18541 aacagctctt gctgcttcat ccaccattct tgctggtata ccagctttta ttgtatctaa
    18601 tccagcttgt tgagcctcta ctgcaatctt aaacacttct ttttgtcttt ctgttggttt
    18661 acctataata aaagttcttt cattttctgc tctatagttt tcataccaaa cctgtctact
    18721 atgtattact atatctcctc tttgaagtat cctagtattg gaatctaaat gaggttgtgc
    18781 tgttctatca atccctgaac aagtccagtt tgcaaatcct atatatgtat caggataatt
    18841 ttcagaaaca tattttagta aagcattatc ccccgcaacg tcaaactcca attcactcat
    18901 cccaactcta acattttcta aagacccttt tattcctata tcagataggt aacctgctat
    18961 cttcaaaaat tctatttctt ttgcatcttt tacatatctc atttcaaata ttttacttcc
    19021 aacatctttg atagcaaatg acttatcagt tatatactct gaaaaacttg cagatactat
    19081 atctttttct attccaacag ttgttccttt agggtattta tttaaaatta tatctaagta
    19141 atgtttatgt gatattccat gttctttcat ttctggtatt tcataataca catatacatt
    19201 atcaactttt gcaacttctt tagcatgtaa ttcttctaat ccaggtacta ttaattctat
    19261 tttatcttga gttactactg ttacaattgg tctagaataa gttattgctt taaatccact
    19321 aatataaaat tgattgtctg gttcaaatat taaagcaatt tcaatgcttt tttcattcat
    19381 gtactttctc aatctttcta gtctcatatt atttgacata atgcacctct ttcttaatat
    19441 tcagataatt tattcaatac aacaaggata aatatattat tttaaaattc ttaatatttt
    19501 tagtacttgt cgtatttgca tgtaaattaa ttttatttgt gattatttta tcataatcat
    19561 gattattttg tcaatcacga ttttattttt aaaatttttt tatattctag tgattttaaa
    19621 ttatctaaaa tataacgagt atacattgta ttttggaata ttattcttga tttatggtaa
    19681 gtttaatgat aaaatcacga ttatataaac tatattgatt atttgtaggt gaataaaatg
    19741 acaaataaag aattacaaaa gaaaaggata ttgatgtatt tcatagaagc tgctcaaaat
    19801 attatggaaa acgaaggtat agaaaatatt acacttcgta aggttgcaga tatggctgga
    19861 tataatagtt caactctata taaatacttt aaaaatttag accatttaat atcatttgca
    19921 tctataaaat attttaaaga ttataacttg aatatttcaa gatgtataga aaatgtcaat
    19981 gatgaatata agaaatatat aattatgtgg aaattatttt gtaagcattc ttttgacaat
    20041 gctcaagctt tttatcaggt attttttaac ttgtctagtg atgaacttag ctatataaca
    20101 aaacaatatt atgatatgtt tccagaagat ttaggtattc atgatagtga catatcttta
    20161 atgattacag gagaaagtat aaaggaaaga aataagatac tattaaacaa tcttgtagat
    20221 actggatata taccagctaa agatttagac ataatcaatg atataattat ttctttttat
    20281 caaaatctat tattcttaaa aaagaacaca ggaaaaaatt ctggtgatga tgaattaact
    20341 atgaaaatga tttcttctat tgaatacata ataaaacatg ctaaataaaa ttaaaatatg
    20401 gctgtctcaa aatagagata agcctttttt attgttgaaa taataaaaat atatttaatt
    20461 tcatataaaa aaaagtgcct cgtaaactaa aaagttcatt ttgagacacc cctaaaacaa
    20521 atttattatt tattatacaa tagtttatat atcatcacct aaaactactt cttccatttc
    20581 atgcttttta cttctcatca ttagttctat tccaacttct ttaggatttg aacctttatt
    20641 tattacagaa tatatagcat tagttatagg catatctata tctaattttt cagcaacatc
    20701 atgtgctact tcagtagcag ttatgccttc tacaaccatc tttacttctt ttaaagtatc
    20761 ttctaaactc atacctttac ctatgagtat accagctctt ctatttctac tatgcatact
    20821 tgtacaagta actattaaat ctccaatacc tgaaagacct gcaaaggtag acatattagc
    20881 tcccatagca attccaagtc tgctcatttc actaatacct cttgtcataa gggcagcttt
    20941 tgcattatca ccatacccta atccgtcaca tatcccagct ccaaatgcta taatattttt
    21001 taatgctcct ccaagttcta ctccaactat gtctggattc gtataaactc taagtttagg
    21061 gctcataaat aaatcttgta tcatctgtgc tatttttaaa tctttagaag caactactac
    21121 ggtagtagga atatcccttg ctacttcttc agcatgagat ggcccagata gtattacata
    21181 tggattttgt ggtaattctt cttcacagac ttgagataat ctaagcccag tccctttttc
    21241 aagaccttta gctacattaa caataacttg tccttctttt ataaatggtt ttattttttg
    21301 gcaagtactt cttacagcct gtgatggaac tgctaaaact attaccttac aatcttttat
    21361 agttttttct atatcagtac ttatagttat attgtttgga aataatactc ctggtaaata
    21421 gtctgtattt tctttagttc tgtttatttt tttagcttgt tcctcttttc gagtccacat
    21481 gctcacatca ttaccttttt ttgcaagccc taaagctaat gcacttcccc agcttccagt
    21541 acctaataca catactttct ccatgtttaa caccacctga gtataatata tttactattc
    21601 tttcttttga ccaatttttc tctctgtccc atttaataat cgctttatat ttgctctgtg
    21661 attatatatt actgatgcag ttaagaacag agctactatt aaaccagctt tattttttac
    21721 cataatcata aatattggtg aacatcctat tcctacaaca gaacccaatg aaacatactt
    21781 tgttatagct accaccaata taaatactgc tagacacatt agtgttatta ctggatttac
    21841 agctagcatt gcacctagtg aagttgcaac acctttacct cctctaaatc ctaaaactgc
    21901 tggccaatta tgacctgcta caacacatat aactgccaga tatccagcta aaagagtatc
    21961 tattccaacc aatcttgctg caaattctga tattaaaaca gccattacac ctttaagaac
    22021 atcacctaaa aatgtcattg ctcctgctct ttttccaagt gtcctaagta catttgtcga
    22081 acctgcatta ccagaacctt gagtccttat atcaacccca gcaattcgct ttgcaactat
    22141 gtatgaggta gatatatttc ctaaaagata tgcaactaca gcaataatta tataactaaa
    22201 tatctccact gaatttcccc cctttagtta tattttaaga tatttatttt ttattttttt
    22261 gtctatactc aaactttatg gaagttcctt caaatccaaa attttctctt atcttattct
    22321 caaggtatct ttgatatgag aagtgagtta aatccttatc atttataaac aatgtaattt
    22381 taggaggtct aatgtcagtt tgagtcccat agtaaatttt tagtcttcta cctttgtcag
    22441 atggtggttg atttaacatt acagcttctc caattacttc attcagtgct gaagttgata
    22501 ttctcttaga atgttcattt gacacatatt caactgtatc tagtatcttg ttcattcttt
    22561 gattagtttt tgcagaaaca aatacgattg gagcatacat cataaatgga aatttttctt
    22621 ttatatcttt tgtgtaatta ctcatagtct tattatcttt ctctattaaa tcccatttat
    22681 tgattacaaa tatgcatcct tttccttcat catgtgctat acctgcaacc tttgtatctt
    22741 gttctgttac accttctaag gcatctatta ctatcaatac tacatctgct ctatctacag
    22801 cactcatagc tcttattaca ctgtattttt ctattgtctc atatatttta ctttttcttc
    22861 taagccctgc tgtatctatt agaagaaatt tctgtccatt tttttcaaaa taagtatcaa
    22921 ttgcgtctct agtagttcct gcaattggac ttacaattac tctttcttct ccaagtatct
    22981 tattgagtat agaacttttt cctgcatttg gtttacctgt tatagctact cttattatat
    23041 cttcttcata ctctgtatct aatcctgctg gaaaattttc aactatctca tctaataaat
    23101 ctccaaatcc catactattg gctcctgaaa ctgcaaaagg cgttccaaat cccaactcat
    23161 aaaaatcata tatattatca aattgacttt gactatcaat cttattaacc actaagatta
    23221 ctggtttttt tgtctttcta agcaattgtg caatttcttt atcagcagct gttattccag
    23281 ctttaccatc aactataaaa agtattacat gtgacatgtc cattgcaagc atggcttgat
    23341 ttctcatttg agataatatt atatcttcgc tatcaggttc aattcctcct gtatccacta
    23401 atgtaaaata tttatctaac cactctactt cagcaaatat tctatctctt gtaactccag
    23461 gtgtattttc aactattgat atcctttttc ctgcaaattt attaaatatt gtagacttac
    23521 caacgtttgg tctcccaact acagctacaa ctggcctact tatactcatt ttatttctcc
    23581 ttccatcttt tgttttctat tttaaaattt tatccacaaa gtcttttcct tcattcaaac
    23641 aagggataat ttttattctc ataagttctt ctaattgatt taattcaata ttatctaaga
    23701 aaatttcttc atctgctttt agcatacttc ttgttatata aagagcttct cctaattctt
    23761 tatcttcaag ttgttctttt aaatccttag ctgttaaaag acctgataca gttattgtct
    23821 ctccaaaaaa cttgtttttg atttcatata catttacttg aagattttta aacttgtcca
    23881 tcatagcatc tgccatactc tgtataaatt catatgcaga atgaccagta gctattgata
    23941 ctttcttctc tctttttaga atattctcag gcaatatatt tagatactct tcaatctctg
    24001 tttttaactt acgtatcata cctacaccat cttcaaactg taaaaaacct tcatattctt
    24061 catatccagg caattcacta ttagatagta tataaaattc atctgataaa aatgcaaatc
    24121 gagtacctaa tttttcaaga tatttctgtt gtatttgatg tatttgctct atagttttac
    24181 ttgcgctctt atcattaaat atatttaatt ctactagatt ttctctatgc tttgtaatcc
    24241 caactggaac tatcgcaaca ctgtttacat atggataaag ttgagctagt tcttttattg
    24301 ttctatccaa ttcctttcca tcatttatac ctggacataa aacaatttga cagttcattt
    24361 ctatatgagc atctgccaat ctcttcatta taccatataa ttttcctgca aatttattgc
    24421 ttatcatttt ttgtcttaac tctggattag ttgtatgcac agatatattt ataggactta
    24481 ttctatattt tataatatta tttatatctt cttcactcat atttgtaagt gtgacaaaat
    24541 ttccttgtaa aaaagataat ctcgaatcat catctttaaa ataaagagtt tctctcatac
    24601 cttctggcaa ttggtctata aaacaaaaca tacacttatt tctacagctt ttagcttggt
    24661 caattatcgg atttgtaaat tcaacaccca aatcttcatc atagtctttt tctatctcgt
    24721 atatgtagac ttcgccatct tttttttgta tttctacatc taaatattca tcacttagta
    24781 agaatctata ctctattata tcatgtattg gctgttcatt cacagatata agtaaatctc
    24841 ctacctctat acctatttct tctgcaatac tatctttata aaccttactt ataatattat
    24901 ttactttttt tacatttact tccaatattt tcaccactct tttgtattca tcaatgctta
    24961 ttataatata ttttaatatg caatttattt atttattata ttttatgata gcatttttaa
    25021 gcatttcatg tcataataac acattttaat tctaatagtc aatataagta aatagctata
    25081 aaatagatta gaacaaataa tattcaaatt taagtttctt aaaaatttga atattatttg
    25141 ttcttaaaaa aattttataa atttaatgtt ttactatgat taacgtccca tttgtcatat
    25201 catgaaccat tccacctaca cactttgaca tcaacaatgc cttattttgt aagtctttct
    25261 catcatttgc ataaaatatt ggacaacctc caccattttt catattctta tcaagtgtaa
    25321 ctatagctag ggtatattca tttaaaccta tatccattcc catcttatgc acttcctttc
    25381 tttcctattt tataattttt tagtgataag ttttttcctt tagagcttga aagtattggg
    25441 catgacttta cagcttccat tactttttcc atattcttat ctactggtaa ataagcaatc
    25501 attatacttt catcctcttt atttcttcta ggtaatggta cgaatgaagg ttctccataa
    25561 tctccatata aacctaacct tgaatatata ttgtatacta ttgcttgtct ttgaccggag
    25621 tcaaataata ttcctgcatt agcataatcc ttatttttag gaattatttg tattcctaca
    25681 cctctattaa tatatacttc tctatcactt tttagaccta tatttgttat accttttaaa
    25741 tctcccacct ttagtataga ttcgtccaca aaactaattt cagcaggcat tacatcagct
    25801 atgtctccaa tactttttct agtaagaagt ttttttaata taaaggctaa cactaatcca
    25861 caaattgaac ttattaccaa actcatccct tcacttattt tcgtttctgc aagtattaca
    25921 taatacaatc ctattgttaa aaaagatgta actatacaca tataatttct tacttcatag
    25981 gttctagcta tctcttctat gaatgcagca cctctcttta caagttgaac ttcctctaaa
    26041 ttttgcaatg tatttcttct atttgctctc acttgtctaa attgttctgc tgctagagat
    26101 aaaaatgtta tggatgtata agaacgattt ataagagctg gaaccaataa tgctcctaat
    26161 gaagaagcga cgaatgctaa tataagttga gatagtaaaa catttggttg cgttggatac
    26221 tgtttttggt ctaaattcaa cattataatt ctagatgtta taccaataat aacagcaaca
    26281 aaaaatactc tatccattaa atcactccta aaatatttaa tttgaaattt tacatgtaaa
    26341 tctacaaagt ctttctagaa aaatcactct gctttccttt tgatgtttct atgataggtg
    26401 tacttttcac tacatctatc atgacatcta tatctttgag tattggcata tatggtataa
    26461 ccacagtact tttttctaaa ttggtcttag atatagcaac aatatcaacc tcatctacat
    26521 ctctatctat ccccatatga ataaatagat tatgtattat agcttgtctt tgccctatat
    26581 catttactat tccaaatgca cctaaatctc ttggtataac ttctatggcc agacattcat
    26641 ttctatactt ttctcttgta ctttctagac ctatattagt tattattaca tcatttacca
    26701 ttaaaatagg tccttcaaaa cttatttttg caggaactat atctgctata tctgctatac
    26761 tgtttcttct taaaaatctt ctaaaaatca agccaacaat agctcctgac acaatagcta
    26821 aaattgtgca atgaaaaaag ctaaggtcat actttcttgc aaaaacgata taaactatcg
    26881 aagatactag tgcagaaaaa agacttatat aacttctaga ttcatacgta gatgctattt
    26941 cttcaacata agcatctcct tttggtacta attcttcatt atcaatattc tttagtgtta
    27001 ttctttcttg ttccgcaaga ccttgaaact gttgtattgc tactgcaaaa aatgtcaagg
    27061 ctgcaaactc cttatctata agtgcaggta atgctatagc acctaacgaa gctgaaagcc
    27121 cagatataat tatctgttct aaataatctt gtggtcttga tgggtattgt ttgtctgtaa
    27181 ctcttaaaac taatcctcta cataatattc ctacaactaa tgcaactata aaagagtgtc
    27241 taaataaatt tgcattcaat aaactttctt ccattttctc agctccttct cttatatagt
    27301 ttgtgcaaaa aacaaaaaaa attcccttct atttaaggaa atttttttgt aatattgtaa
    27361 ctataaattc gctaccaatg ccaagttcac tttttacttt tatacatcca tttagtgatt
    27421 tgactatatg ctttgttata gccaatccta aacccgttcc tcctacatcc ctacttcttg
    27481 ctttatctac cctataaaat cgttcaaata ttcgttctac atcttctttt ggtatcccta
    27541 caccattgtc tataacttta attacgattt tttcttcatc ttttaacact acaacttgta
    27601 caatcccatt atcttgggta tactttatag cattatcaac taggtttaag aacacttgct
    27661 ttatatagtc tttattggat agtatactta ttgatttatc atcacattta tataaaactt
    27721 ctatattttt ttcactagct gaatgttgag ttatttcata tacctcttta aatacatcat
    27781 atactacaac attttccata agaagagtat ctttattttc tacaaatgat aacagcaata
    27841 tatcatcaat caaccttttt aatctatctg attcgctttc tattatttct aagaatctat
    27901 ttctagtatt cacatctata ttttcatttg actttagtgt ttcaacaaac ccacttatag
    27961 atgtaagtgg tgttttaagt tcatgactta catttgcaac aaaatcactt ctcatatttt
    28021 caagtttaac ttttttagta atatcttcta tatttactat cgaacctatt attacgttgt
    28081 tagtattctg aagataaact gggtctacct ttatcttata tacgatatca tctttagtag
    28141 taatattagc agtttcatta tctttagaac caataaattg aagtatttgt tttaatattt
    28201 cttcttcttt tattacaaca ttaatattat gaccttcaac taattgattt ggttcactct
    28261 ttatcatttc ttttgcttca tcatttatca gaagtatatt tccatttaca tctatagcca
    28321 aaattccatg agatatactc tttaatatag atcttaactg taaatgttta tactgtactt
    28381 cttctaaggt tttatccata ttctttatca tatgattaaa atttctagct aattcaccaa
    28441 gttctccttt ggctgatatg ttaagcctag catgatattc attattactt actttttttg
    28501 agacgtatat aaactctttt aaatatttcc ttaatgttat tgtatatctt acagctaata
    28561 taacaatggc tatggacaac ataacaataa aaaaatatat actatttaaa tcttccacaa
    28621 gataacactc ctaaaatata acctttttaa tctattttat accctacacc tcttatagtc
    28681 tctatatatt tttccgattt atcttcatct tctatttttt ttcttaaata tcttatatga
    28741 acatctactg ttctagtttc tccatagtat tcataccccc atattttatc caaaagataa
    28801 tttcttgaaa gtacttttcc tttattttga attaataatt ttaacaattc aaattcctta
    28861 agagttaact ctatcttttc agagcctttg gtaacttcat gttttgataa atctagtttt
    28921 aaattgcctg tagtaagtat attgttttct tcacctaagc tagtcaaatt atatctcctt
    28981 aaaacagaac taatcctagc taacaactct tttatactaa atggcttagt tatatagtca
    29041 tcagcaccta tttcaagccc ttcaaccttg tcattttcca tatttttagc agtaagcatt
    29101 ataacaggaa tattttttaa gtccttatca cttcttattt ttctcaaaac ttctattcca
    29161 cttatatttg gtaacatcca atctaaaagt attaaatctg gtttaatttc cttagccttt
    29221 ataaatccgt caaatccatc atatgaataa ctaacttcgt aatttgacac ctccaaattg
    29281 aattttaata gctcaactat atgcatttca tcgtctataa caagcacttt agtattcatt
    29341 aaaaagctcc tctctatttt atctgtagta ccgttcctat tcgttctgat gccttattat
    29401 gttttctata agagtggaat ttatctgctc tacaactagt gcataaattt aaatttatta
    29461 tattttcttt tttaactcca ctacatctaa gcatatactc attaattttc cataaatcta
    29521 aatagtaact tccctctttt attatataga acttttcatc attatttgta agaattgtgt
    29581 taaatttttc tactaaatct tcagacactt catagcaaca tggtccaatt gatggcccaa
    29641 ttatacatac taaatcttca ggaactgttt tatagcattc agacattaat tttattgttt
    29701 tatgacctat attactaaaa gtccctctcc aacctgcatg agccagtcct atggctttat
    29761 ttttagcatc tataattgat ataggtacac agtctgctgt aaacagtaaa agtggtactt
    29821 tttccaagtt tgttataagt gcatctccat ctcttctttt ccctatagta tctttattga
    29881 ctatattaac tatatctgaa tgtatttgag aattactagt tatattttca aatgtcaaat
    29941 cacattcttt acatactttt aacatatctt ctctattttt tgcatctata ttcttacatg
    30001 ttataactag atttacagaa tttaattttt catcaagttc ttcacaatta ccacttaaat
    30061 taacatactc tttaatatct tgtacatttt ttatatttat ataatctttc atcatttgtc
    30121 tcctttttct atcagtatac ttttaagttc atttacaaaa gtatttatat ctttaaattg
    30181 tctatacaca gaagcaaatc ttacataggc aacctcatct aactctttca atttatccat
    30241 taccatctca ccgatttttt tggtttcaat ttcttctata aaacatttat ttatttcatt
    30301 ttcaatatgt acaacaatac tttcaatctc ttcaatagaa acaggtcttt tttcacatgc
    30361 ttttagcaat ccatatttta tcttttctct atcaaaatat tctctactat tatccttttt
    30421 aatgaccatt aaaggagttg tttctatttt ctcatacgta gtaaacctct ttttacatga
    30481 ttcacactca cgtcttcttc tgatagattt taaatctgta tgtctagaat ctattacctt
    30541 tgattcttta taattacaat aaggacattg catctatatt cctcctttgt ttaactatat
    30601 ctcaaactca tctaagctta attctgaacc tatatttact attattgtat cacaacctat
    30661 tttaagtata tcattccaaa atataacttc ttcatcccct cctcttgaaa aaaaacttct
    30721 acttccatct ccaaaaatgg taaatgagac taccttacct tcgtgaatat ccatgtctat
    30781 gtctattata aaccccatcc tttttccatt ttttacattt ataatttctt tttccattat
    30841 atcagaaact ctaatcatat tgtactcacc ctttctttaa aaataaatat gtatcgaagt
    30901 aaaaaaaaat gacctatata ggtcatttaa attaaaataa tgtttacagt ccatggatta
    30961 cttatacata ttttctcata ttttttaaag catttttttc aattcttgat acttgtgctt
    31021 gagatatacc gatttcatca gcaacttcta tctgagttct tcctttgtaa aaccttaaat
    31081 ctaataccaa cttttctcta ctatttaatt tcttaatagc ttctttaagt gatatttctt
    31141 gcaaccaatt ttcatcagta tcttttttat cttgaacctg atccataaca aatattgcat
    31201 ctccattgtc ttgatatact gggtcaaaca gagatattgg gtcttgtatg gcatcaagtg
    31261 ccattacaac tgattctact tctaattcta attcctttgc tatttctgat actgtaggtt
    31321 ctttagaatt tgttctaatt aatctttctc taacttgtag tgccttatat gctatatctt
    31381 ttaatgacct acttactcta attgggttat tgtctcttag atatcttcta atttctccta
    31441 taatcatagg aacagcataa gttgaaaacc ttacattttg gcttaaatca aagttatcta
    31501 tagcttttat aagaccaata caacctattt gaaataaatc gtctatattt tctcctctgt
    31561 tgttaaactt ttttattaca ctcaagacta atcttaaatt tcctcttaca aactgttgtc
    31621 tggcttcttc atcaccattt tttatttgta ataaaagttc cttcatttgc ttatttttaa
    31681 gaactggaag ttctgatgta ttaacaccac atatttcaac cttattaact tgcatatatt
    31741 ttcagtcctc tctcaaaagt attcatctat tgaaattatt tacatttcca atttttttat
    31801 actttttaag aagaattaat ttaacattat acaaattttt tcatttcttt ttgcaacctt
    31861 gagattattt tcttttcaag tctagatatg taggactgtg atattccaag cattccagca
    31921 acttcttttt gtgttttttc aataccttta tcaataagac caaatcttaa ttccataatt
    31981 tgcttttctc tgtcacttaa tctgtcaagt gccataacca acaaatctct atctatttct
    32041 tcttcaatta ttttgtatat ttcatcattt tctgtaccca atacatcaga taataaaagt
    32101 tcatttccat ctaaatctat atttaaaggt tcatcaaatg atacttctgt tttcttttta
    32161 ttattttttc ttaagtacat taatatttca ttttctatac atctcgaagc ataagttgcc
    32221 aattttatat ttttattcag tttaaaagta ttaactgctt ttatcaaacc tatagttcct
    32281 attgaaatca aatcttctac atctatacct gtattttcaa attttctcga tatgtagact
    32341 actaatctaa gatttctttc tattaaaatt gactttacac tttcatctgt ttctagcttt
    32401 tgtaaaagct ccatttcttc ttctggtttc aatggaggag gaagtatatt tgcccctccc
    32461 atatagtata ttccctttgg ctttattatt tttaatttaa tacctaacat agtaataaag
    32521 cttattattc tttctttcaa tcgtaacatt ccttgtcccc cttaaatttt cacctaaaat
    32581 ccaggcaata tatttggatt cattattgct ccatattctc tatcattaaa atttgaaata
    32641 ccaagtatta tatttcctat cttttgctca tctacttcta tatagtcagc ttttaagcct
    32701 attattatgc ttgtcttatt acttccagca tgtttataag gtattattct aactctacct
    32761 gaagttttat catctaaact tcctataatt tcttgtgctt tcttcatatc tatatcttca
    32821 taattaatat ttaatagttt ttctggaata aatccttcta acacactaga cttaactatt
    32881 attacatcac tttgacttat tgggtctttt aacaaattac cactatctac taaagcttta
    32941 cagcaaaaac ttttttctag taagtttata tttatagtct ttgtaagctc cttaatgtac
    33001 ttaagcattt tcaaatcttt ataaatgtat tttaataatt cacaactcac ataagtacat
    33061 gcaattaaga aagatatttt taagtgactt attcctgtaa aataaattat aaaataagtg
    33121 cttccagaaa taaaaatgtt tactagataa aaaactaacg ctacatgaac ataatctttt
    33181 ttgctcttgc ttctaaatga aattaaagtt attatgctca tgattattat tttaaatggt
    33241 acagtaaata gtatattcat tgatggatac aaataagcaa cagaataggc acttcctaaa
    33301 ctagccccca ataacttatt ttttttagga ttatatcttt tagtaagaat agaagtacaa
    33361 cttataataa tataatttat tactaggttt tctataacat agtactcaat atacaccatg
    33421 tatcccccct taaaaccttt aaacatatta taagatagta tgtacaaaat tttatgtcac
    33481 ttttgatggt cgaatccaaa acaaattcta tttaaaaaga acaatgatat atgtcttata
    33541 ctttatgtcg tcaaaagata tttttttaat gccaaacaga tagacttttt tgtagaatcc
    33601 aatatatcta ggtagaagtt attcgcttta cttttggtat atttataaaa aaaagactac
    33661 ataatttaat aatatatcaa gtaaatcttt tacttatata aaatcaaatt atatagtctg
    33721 taaattcaaa tataaattat tgtgctagta atccatttgc atattttgca gattcattta
    33781 gtgcttgttc tggagttatg tctccagcta tcatcatttg aagttgtttc cataaatatt
    33841 catatatctc tactccatga ggtccaactg tcaatggtct atacatatgt ttatctcttg
    33901 tcaccatttc ttcaaatctt gggtctcctt ggtatttttc acctttagca actggagctc
    33961 taggtatttc tttgtggaaa gcttccattg attctttgct taacatatac tcaagaagtt
    34021 taaatccaag ttctttattt ttacatgctg acattaatgt taaatcatct actgaagcaa
    34081 aacttccaac tgtcttatct tctaatgatg ttacatatcc ccagtttaaa tccgggaatg
    34141 atttatcaaa tgtacttgta gcagcagagg atagcattat agtaaatgca gcttttcctg
    34201 gtccaaatac actttcaatc atttcattac tatcttttga taaagcatct tctggaatat
    34261 actttttcat atcatttata aagtttgcag ctttcaagcc ttctgcatta ttaaatttaa
    34321 ctgattttaa atcatcatta tatatatctc caccagcttg ccataaatac ggataccagt
    34381 tccagtttaa gcttccaaat actttagaac cccaaccttg tgcaagaccc cactggtcga
    34441 tttttccatc tccatcagta tcttttgttg ctttttcaca gattctttta aaatcatccc
    34501 atgtttttgg aggtttttct cctaattttt ctagtatatc tttattgtag tacaatacgc
    34561 ctggattagc agcttctata ggtaaaccat aaataccacc catcattttt gcatcatcta
    34621 agtatagata attatctgaa gttcctgatt tttctaaata aggtgttaaa tcttctactg
    34681 cacccatttc tataaattga gggaacatct ctgcatacat gtatcctata tctggacctt
    34741 cacctgcact tattgctgtt gcatattttt ctgcgtagtt atcccatggt actatctgaa
    34801 attcaatttc acagttattt tctttttcaa atttttcaaa tatcggcttc catgcatctg
    34861 catcatggtc tgttaatggt gcagaccata caactaattt ttctttaccc tctttgcctt
    34921 tagaatcttc tttttttgaa catcctgcaa gaattgatac tgatagaatc cctaccataa
    34981 ctaaagaaag aacctttttt agtttaatca tgcttaatcc cctttcagac tttaatttgt
    35041 ctttttcctc aaatgtttac atttaattca gatttaatat taacaaataa gactactctt
    35101 gtctaatcat aaaaaactat actaactcct ataactatat tttttttcta tacctattag
    35161 atttaattca tactaaaaac ttacttttta aattttatca atatttaaaa atatttttaa
    35221 gtctagccac aaactaacta tctaaattga acaaaataaa aagaagtcta ttgacttctt
    35281 tataagctta catattatct tcttcttctt aggaatgtag gtatctccat gctctcatca
    35341 tcatcttctt caattatact tgccttttta ggaggctcta ttttttcctc agcaattact
    35401 tcttcttcaa tttcttttac agcttgtttt actgttgtat ttaatgaaga acgtatgctt
    35461 gatttaggtt tagtatctaa atctaaggca ccattttgta atccttcaaa tcctgttgcg
    35521 attactgtaa tcattatttc atcacctaaa tcttctttta tagaagctcc aaatataaca
    35581 tttgcttctg ggtcacagga ttccataact aaagttgatg cctcattaac ttcaaataat
    35641 cctaagttag gaccacctgt aacattaaga agtactcctt tagctccttg tattgatgtt
    35701 tctagaagtg gagattgaat agcttgtctt gcagcatcaa ttgctctagt ttcaccacta
    35761 gcactaccta tacccatatg agcaagtccc ttgtctttca ttatagtagt aacatctgca
    35821 aagtccaagt ttattaaccc ttcaactgct ataaggtctg aaattgattg tataccttgt
    35881 tttaatacat catctgcaac tgcaaaagca tctaacattg atgtattttt ttgtactatc
    35941 tgtaaaagtc tatcatttgg tatcgttata agagtatcta cttttgattt aagttctgct
    36001 attccacctt cagcgttctt cattcttatc ttaccttcaa atgcaaaagg tttggtaact
    36061 acacctactg taagtatccc catttctttt gccaagcctg caaccactgg agcagctcct
    36121 gtaccagtac caccgcccat tccggctgtt acaaagacca tatcagctcc ttgaagtaac
    36181 tttactattt catctttact ctcctcagca gctcttttcc ctacttctgg atttgctcct
    36241 gctccaagtc ctctagttaa tttttctcct atttgaactt tatattcagc ttttgatgta
    36301 tataaagctt gtttatctgt atttactgat ataaattcaa cgccttttag ttgagcttct
    36361 accattctat tgacagcatt gtttccaccg ccacctacac ctattacttt aatttgtgca
    36421 cattcttcta attctacgtc aaagtttagc atcataaacc cccttctctc atgattcaaa
    36481 gttatcgtat gtattaatta tatcattttc ttcttttatt ttgttaacct ttaagtaaca
    36541 tattccacaa aaacgtaaat tttccttttt aaatttctta cttatattgt caaatattac
    36601 tctttaagta taaaataatt tacgtatata ttaaatgctc tttaattttt acgttttttg
    36661 ttcataatat tactaataat atatcgtcta attatagata agttattaaa caatctcata
    36721 ccaaaaacta gtacagcaac atagtaaaga gaaactccta acttttctcc tatataagtc
    36781 aataatatag ccaatatagc attacctatg aaaccagtaa taaaaattac attatcatag
    36841 ttatcttcaa aattggattt tatcgctcca aaaatagaat ccattattgc aagaatagct
    36901 actgacatat atatggaata atccatagaa tatgtaaatg gaatatagta acctactact
    36961 acaccaaatg ctaatcctcc taaaacccac aacataaatc ttcacctctt tattttactt
    37021 tgaaacacaa ctacattaca atccttttta ttaatttttt ttactttttc tttatttttt
    37081 tgtatttttc gttttatttt gcaaataaat tcactttatt cccattattt tcctgaactt
    37141 ttatattaat tccatatata tccttaagag atttagcata tgattctgga gagtttatag
    37201 ctgcactcaa taaatctata tctcctatag cttttattat aaatggttgt ccatatgtag
    37261 tatcatttac tgtaatagta gctcctgaac attttatctt acttgttgct agtactcttt
    37321 ccccatttat agaaattgct cttgctccag ccttttttaa atcatttaaa actctcaaaa
    37381 tatctatatc atgtataatc aaatcatttg gattctgtcc gtcttttaac tctctctcac
    37441 tatccctcat agtaatagtt atgcctggcc ctgtaacagt tgaataacca cttaattctt
    37501 taacttcttc aagctctttt tgcataagtg tttggataga ttcatttcca ttcttttttg
    37561 cttctttata ttcattcaaa gtttttttgt tgtttttaag agtttctttt aaatttttta
    37621 tttctttttt tgtattttct acctcacttt caatactttc agtttgacct aatgttatgt
    37681 atactctatt tgggtctagt gattttatat aagttgatat gaaaatgcct aatatgaatg
    37741 ctcctaatat aactattttc tttttcatac tattatttcc cctctgcttt gtttatatca
    37801 ctatgaccat ttatttctat attgtcttct ttggtcactt ttactttggc attataatat
    37861 gtttctattt cccagacaat accatacttt atattaagtg ccgaagccaa tgtatcagaa
    37921 tcaccaattg cttttattat aattggttct tttatgacag tgtcattcat acaaagcttg
    37981 tcttctttta aatacatata tgtattaaaa actattctct ggtcgtttat agatatggca
    38041 cttgcttgtg ctgaatttaa tttatttatc attgacaata ataaatcata attatatagt
    38101 atactatcat ttttatcaga gtttcctaag tcttttgtag aatttatctc aatttttatg
    38161 cctgttcctt caacatctgt atatccagct aatgtttcat atttttctat ttctgactta
    38221 agtatgtctt gttctccttt attgtttttg cctctataag tctctatatc tttcttcaca
    38281 ctattaactt ctgattgtag attctctttt tctgttttta aagatttaag ttcttctgta
    38341 agttgctctc ctcttttaaa agttgtcatc cctctatttt ctatattaat aattttaagt
    38401 tgtaaactaa caagaacacc caatacaaca cttcctatga ttatactttt atttataaat
    38461 ctttttttca atatatcact tccttacaat tgtctatcct tctggactat acactgccaa
    38521 ctttccatag ttaagattta tagtccctcc attagtattt ttattttgca ggtcaatcag
    38581 aattttactt accctagata tattataatc taagttatca tcattagaaa gtaagatttt
    38641 tatattactt cttgtaagca tttctatatt gctttctttt tttaagttta cataatttat
    38701 ttttctataa attccttctt cttttaaata atttaaagtt ttaaatacat tctccttagt
    38761 tttataattt ttaaatttta tagatttatt atcatcaacg ctataatcta catctactat
    38821 caagt
//
LOCUS       DAELSY010000021        38528 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.021, whole genome shotgun sequence.
ACCESSION   DAELSY010000021 DAELSY010000000
VERSION     DAELSY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38528
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.021"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            109..300
                     /locus_tag="KPG09_000965"
     CDS             109..300
                     /locus_tag="KPG09_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBG0965589.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            455..1864
                     /locus_tag="KPG09_000966"
     CDS             455..1864
                     /locus_tag="KPG09_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0965590.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIRIFKDKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKICGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            1995..2684
                     /locus_tag="KPG09_000967"
     CDS             1995..2684
                     /locus_tag="KPG09_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBG0965591.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2687..3622
                     /locus_tag="KPG09_000968"
     CDS             2687..3622
                     /locus_tag="KPG09_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBG0965592.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3619..4512
                     /locus_tag="KPG09_000969"
     CDS             3619..4512
                     /locus_tag="KPG09_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBG0965593.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4590..5336
                     /locus_tag="KPG09_000970"
     CDS             4590..5336
                     /locus_tag="KPG09_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBG0965594.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRASSRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5337..6170
                     /gene="rsmI"
                     /locus_tag="KPG09_000971"
     CDS             5337..6170
                     /gene="rsmI"
                     /locus_tag="KPG09_000971"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBG0965595.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6193..6897
                     /locus_tag="KPG09_000972"
     CDS             6193..6897
                     /locus_tag="KPG09_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBG0965596.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7085..7513)
                     /locus_tag="KPG09_000973"
     CDS             complement(7085..7513)
                     /locus_tag="KPG09_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG0965597.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7550..8980)
                     /locus_tag="KPG09_000974"
     CDS             complement(7550..8980)
                     /locus_tag="KPG09_000974"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBG0965598.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9112..9696
                     /locus_tag="KPG09_000975"
     CDS             9112..9696
                     /locus_tag="KPG09_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HBG0965599.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9712..10233
                     /locus_tag="KPG09_000976"
     CDS             9712..10233
                     /locus_tag="KPG09_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HBG0965600.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10299..10545
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10629..12566
                     /gene="metG"
                     /locus_tag="KPG09_000977"
     CDS             10629..12566
                     /gene="metG"
                     /locus_tag="KPG09_000977"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBG0965601.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12596..13366
                     /locus_tag="KPG09_000978"
     CDS             12596..13366
                     /locus_tag="KPG09_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBG0965602.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGICYEEVCRVTKENAKKFFNIK"
     gene            13703..14134
                     /gene="phnG"
                     /locus_tag="KPG09_000979"
     CDS             13703..14134
                     /gene="phnG"
                     /locus_tag="KPG09_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBG0965603.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14149..14724
                     /gene="phnH"
                     /locus_tag="KPG09_000980"
     CDS             14149..14724
                     /gene="phnH"
                     /locus_tag="KPG09_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBG0965604.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTVSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSTINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14727..15812
                     /locus_tag="KPG09_000981"
     CDS             14727..15812
                     /locus_tag="KPG09_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HBG0965605.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRDFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15832..16701
                     /locus_tag="KPG09_000982"
     CDS             15832..16701
                     /locus_tag="KPG09_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBG0965606.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16694..17560
                     /locus_tag="KPG09_000983"
     CDS             16694..17560
                     /locus_tag="KPG09_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0965607.1"
                     /translation="MSKINVSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17601..18314
                     /locus_tag="KPG09_000984"
     CDS             17601..18314
                     /locus_tag="KPG09_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0965608.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMAKGTISESGVA"
     gene            18315..19484
                     /locus_tag="KPG09_000985"
     CDS             18315..19484
                     /locus_tag="KPG09_000985"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBG0965609.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDRIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVLNTIVNGKTVYKREV"
     gene            19488..20324
                     /locus_tag="KPG09_000986"
     CDS             19488..20324
                     /locus_tag="KPG09_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG0965610.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20325..21320
                     /locus_tag="KPG09_000987"
     CDS             20325..21320
                     /locus_tag="KPG09_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0965611.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLDVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21338..23026
                     /locus_tag="KPG09_000988"
     CDS             21338..23026
                     /locus_tag="KPG09_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG0965612.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYIIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23038..24105
                     /locus_tag="KPG09_000989"
     CDS             23038..24105
                     /locus_tag="KPG09_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0965613.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            24591..24707
                     /locus_tag="KPG09_000990"
     CDS             24591..24707
                     /locus_tag="KPG09_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HBG0965614.1"
                     /translation="MDDDINMIAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            24871..25938
                     /locus_tag="KPG09_000991"
     CDS             24871..25938
                     /locus_tag="KPG09_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0965615.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PNSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            26130..26660
                     /gene="rnmV"
                     /locus_tag="KPG09_000992"
     CDS             26130..26660
                     /gene="rnmV"
                     /locus_tag="KPG09_000992"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBG0965616.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMQRIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            26831..27700
                     /gene="rsmA"
                     /locus_tag="KPG09_000993"
     CDS             26831..27700
                     /gene="rsmA"
                     /locus_tag="KPG09_000993"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBG0965617.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREILKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            27766..29037
                     /locus_tag="KPG09_000994"
     CDS             27766..29037
                     /locus_tag="KPG09_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965618.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDSDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEEYEELEEDEEIEDNEESDRDEDS
                     EEEYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSGDEEIEEFNNYKEIENYNRV
                     KVQEEYIDIEYDEEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGV
                     SQGESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMP
                     YLGYLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGST
                     GFLRYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            29188..30303
                     /locus_tag="KPG09_000995"
     CDS             29188..30303
                     /locus_tag="KPG09_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG0965619.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            30489..31385
                     /locus_tag="KPG09_000996"
     CDS             30489..31385
                     /locus_tag="KPG09_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG0965620.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASIVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            31455..32252
                     /locus_tag="KPG09_000997"
     CDS             31455..32252
                     /locus_tag="KPG09_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965621.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(32308..33660)
                     /locus_tag="KPG09_000998"
     CDS             complement(32308..33660)
                     /locus_tag="KPG09_000998"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBG0965622.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            33879..34757
                     /gene="purR"
                     /locus_tag="KPG09_000999"
     CDS             33879..34757
                     /gene="purR"
                     /locus_tag="KPG09_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBG0965623.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            34913..35191
                     /gene="spoVG"
                     /locus_tag="KPG09_001000"
     CDS             34913..35191
                     /gene="spoVG"
                     /locus_tag="KPG09_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBG0965624.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            35401..36780
                     /gene="glmU"
                     /locus_tag="KPG09_001001"
     CDS             35401..36780
                     /gene="glmU"
                     /locus_tag="KPG09_001001"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBG0965625.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEAEVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSNVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SVVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            36875..37825
                     /locus_tag="KPG09_001002"
     CDS             36875..37825
                     /locus_tag="KPG09_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBG0965626.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
BASE COUNT        15461 a         4208 c         6935 g        11924 t
ORIGIN      
        1 ttttttgtca aaaaaaacaa aaaatacgta tttatttttt catttcggga aaactaaaca
       61 caggaattat ggactttata aaaatttaat tcttgaggag tgatataagt gaaaaaagtt
      121 gctttaattt tagctataat aggtgcgctt aactggggtg caattggtat tctaggtaca
      181 gacttaatag gtagtatctt tggtggaaca tatgaaatgg taagtagaat aatatacttc
      241 attgtaggtt tagctggatt atacttaata ccaagtttaa tgtctgatga tagagaataa
      301 tttatgcttt tatgttaaat acctcattct aaatgtttag aatgaggtat ttaattttat
      361 aaaaatatta atttgtttct tttttaatgg taaaatagat aagaattgaa gttttaaaaa
      421 gaagtattat gtaaaaaagg agtactatta atatatgttt attattaatg aattaaataa
      481 gattgtaaat gaagaagtaa tatcttttca tatgcctggt cataaaaagg gacaggttta
      541 taaaaagtta ggatataaag atatactaaa aaatttgtat aaaatggata caactgagat
      601 attaggaaca gataatttac actttccgga aggaattata aaaaattcac aaaaaaaagc
      661 atcaagagta tttaaaagtg attatacttg ctatttagta aatggtagta gctgtggaat
      721 acaatctgca ataatgtcag tatgtagtcc taaaagtaaa ataataacaa atagagactg
      781 tcatcagtct gttataaatg gatgtatttt aggagatata gatatagagt atgtgccttg
      841 tgagataagt gaagagacaa atgtcttaaa agggatatct ataagtaatg tgattgatat
      901 aatagataaa aacttggatg caaaagcagt actgcttact tatcctacat attatggaat
      961 gacatatgat ttagaataca tatgtaatta tgcacatagc aaaaagatgg tagtgatagt
     1021 agatgaagca catggtgcgc atttagggct tagtgagagg cttcctaaaa cagctcttga
     1081 gcaaggtgca gatatagttg tacagagtac acataaaact cttccagcat ttacacaatc
     1141 gtcaatgata catatacagg gagaccgagt tgatatagat aaaatattga acatgttaag
     1201 aattacagaa tcttcaagcc cttcatattt attattatct tctctggaat tagcagtaga
     1261 tatatatgaa aataaaggaa aagaattaat ggaagagctt ttaaataata ttagaatttt
     1321 caaagataaa gttgattcaa atattaatat atatgacaca aatgataaaa caaaaatatt
     1381 tatatcatct aaaaacatgg gtttaacagg ttatgaacta gaaaatattt tgagaaacaa
     1441 atataacata caagtggagt tatctaatta ttatggagtt cttttaatat gtacaatagg
     1501 aaatactgct gaggatttct ctagtttaga aacagcatta aacaatgttg taaaggaaga
     1561 ctttaaattt gaaaagttag aaaataaaaa ataccctgtt gttattccaa agaaaatatt
     1621 aacacctaga gaagcatttt ataaaactaa aaaaagtgtt aaaatatatg acagcatagg
     1681 taaaatttgt ggtgaatgta tagttccata cccacctgga ataagtgtaa tatctcctgg
     1741 tgaagtaata agtaaagaaa taatagacta tctaatgttt tgtcattcta aaggcatgat
     1801 tgtgagtgga ttgaaagatg tagatttaaa ttttatacaa gtaatagatt tagaagacat
     1861 ttaaaaatta tgatataaaa gtagatagaa gtagatagtt taaaattgtg aagagaaata
     1921 aatagactgc ttatatagag ctatctaata aaagtactaa aaaagtaaaa agtgtttgta
     1981 tggaggatat gaaaatgaaa ggtaagttaa taattataga gagtggttca gatgcaagtg
     2041 gaaaggctac tcagacagaa atattatatg aaagattaaa aaaagaaggc tatatgatta
     2101 aaaaggtaga atatccaaat tatgaatctc catcatctgc cttagtgaaa atgtacctaa
     2161 aaggagactt tggtaaaaat gcatcagatg tagacccata tatagcatca acttttttta
     2221 cagctgatag gtatgcatca tttaaaaccg attgggagga gttttacaat gaaggtggaa
     2281 taattatcgc agatagatat acaacctcta atatggtaca tcaggcctct aaaatggata
     2341 tttgtgatag agaagagtat ttaaattggt tagctgatta tgaatttaat ttatttaaga
     2401 taccacaacc tgattgtgtt atatttttag atgtgcctat tgaatttagt aaaaagctta
     2461 tggagaatag aaaaaataaa attacagggg aagaaaaaaa ggatattcat gaaagtgaca
     2521 tagagtattt agagaaatct tacaacaatg ctttatatat agcagataaa tatgattgga
     2581 aaaaaataaa ttgtgtttta gataacaaat taagaagtat tgaaagtata cataatgaaa
     2641 tttataaaat agttcttgat tcaataaaac ctatggagag ataaatatgt attttgaaaa
     2701 tattataggg caagattttg caaagaaata tttaacaaat tctataaaga aaaataaact
     2761 taataatgcg tatatgtttg aaggaatgga tggaatagga aaaaagaaat ttgcagatga
     2821 attatcaaaa ctattacttg attatgaaaa tctcgaaaat agtccagact atgtactcat
     2881 aaaaccagat ggaaatagta taaaaatagc tcaaatcaga aatttacaat cagatatagt
     2941 aataagacct cataaagact ataaaatata tataatcaat aatgctgaaa aaatgactgt
     3001 ggaagctcaa aatgcacttt taaagacgct agaggaacct ccaaactatg ctataataat
     3061 cttagtcaca aacaacaagg aatctttatt agaaactata aaatctagat gtgatataat
     3121 aaagttctca cctataccaa ttgaagactt aaaacgttat ttgataaata caggtataga
     3181 agaagaaaga gctcaattat tggctatttt ttcgagagga agtattgaga atgctctaaa
     3241 tctatctcag tcttctgagt tttcaatgat gagagaagat atacaacagt atattcagat
     3301 aatgttagat aaaaacatag tggagatttt aaatatacca aacaatatgg aaaaatatag
     3361 aggtaagatt atagctttat tagatatgat gataaactat tttcgagata tcatcctatt
     3421 aaaagaaaat gtaaacaaga acatgttaat aaatgtagat aagctagttt tcattcaaaa
     3481 tatgagtgga aaaattagtt attctcaatt gtccaagatt attgatataa tagaagatgc
     3541 aaagagtaaa ataaaaagca attgcaattt caatataagc atacaagtta tgtctttaaa
     3601 tatatacgag gtgattaaat gataaagata gtaggtataa ggtttaaaag tgctgggaaa
     3661 atatattatt ttgaccctgt tgattttaat atagagcaag atatggatgt agttgtagaa
     3721 actgcaagag gcttggagta tgggaaagta gtcgttggtc caaaagatat ggatgaaagc
     3781 gaattagttt ctcctttgaa gccaattata agaatagcta cagaagaaga taaaaaagta
     3841 tatctagaaa ataaggaaaa ggcaaaagaa acttttgagt tgtgccaaca aaaaataaaa
     3901 gaacatgagt taacaatgtt tttaatagat tgtgagtata cgtttgatag gaataagttg
     3961 attttttact ttacagcaga aggaagaata gattttagag aattggtaaa agacttagct
     4021 gctatattta aaactagaat agaacttaga caaataggtg tcagagatga agctaaatct
     4081 ataggaggtc tagggccttg tggtagaaaa ctttgttgtt catcttggtt aggtgatttt
     4141 caaccagttt caataaaaat ggcaaaagac caaagtctat ctctaaatcc aacaaaaata
     4201 tctggaatat gtgggagatt attctgttgc ttaaaatatg agcatgatgt atatagtgaa
     4261 gcaatagaaa aaatgcctgt tgtaggtgca ttggtgcaat cagatgatgg aaaaggaaaa
     4321 gttgtagaag tgaatccttt attagaacaa attaaagtgg aatttcaaga caagacagta
     4381 aagatataca caagagaaga tataaaaata cttagagagc ctaaaaaatg tgatggctgt
     4441 ggtggtaaat gtaaggataa tgatggatta gatgaagcca cactaagaga gctaaagaaa
     4501 ttagaagatt aaatttaagg tagggctttt cctacctttt ttaaagcaat taatgcttaa
     4561 agttattaat atttgaggag aatttatata tgcaattaaa agattcagaa agaatagatg
     4621 atttacaatt aaaaggcctt aaaattatac aagatactaa tggtttttgt tttggtatag
     4681 atgcagtttt attagccaat tttactaaaa taaaaaaaga tgccaaagta gtagatttgg
     4741 gtactggaac tggaattata cctattttga tagctggaaa aagtgaagct aaaaaaatta
     4801 ttggcgtgga aatacaagaa gatgtatatg aaatggcaac tcgttctata aaattaaatg
     4861 gattagaaga aagagttgag attataaatg aagatataaa atacatagac aaagtcttag
     4921 acataaatgg ttaccatgta gtcacatcaa atcctccata tatgcatatt gatggaataa
     4981 aaaatccaaa tgataaaaaa gcaatatcaa gacatgaagt aaggtgcaat ttggaagatg
     5041 taattagagc ttcatccaga ttggttatgc ctagaggaaa attcttcatg atacatagac
     5101 caacaagatt ggttgatata ataactttag ggagaaaata taaattagaa ccaaaggtta
     5161 ttcaatttgt gcatccaaga cctcaaaaag caccgaactt agttttggtt caatttgtaa
     5221 aagatggaag accagagctt aaaatattgg acccattgta tgtttatgga gaagatggga
     5281 attatacaaa agaattaaaa gcaatatata ataataaaga tataggagag ttgtaaatga
     5341 gtgggaaatt gtacatatgt ccaacaccaa taggaaacct agaagatata acatatagaa
     5401 ctttaagaac attaaaagaa gtagacttga ttgcagcaga agatacaaga catagtataa
     5461 aacttttaaa tcattttgaa atctcaaaac cactaactag ttatcatgaa cataataagg
     5521 attcaaaggg agattatttg ataaataaac taattgatgg tgaaaatata gctctgataa
     5581 gtgatgcagg tatgcctggt atatctgacc caggagaaga gataataaaa caagctatac
     5641 aaaataacat agagatagaa gtattgccag gtgcaacagc ctttgtaacg gctttagttg
     5701 gctctggtat ggacacacat agatttgttt ttgagggctt tttagataga gataaaaaag
     5761 ttaggaaagc acaattggaa gaattaaagg aagaaagtag aactataatt ttctatgaat
     5821 caccacatag attgaaggat acactaaaag atatgcttaa aattcttggt aataggaata
     5881 tttctataaa tagagaactt acaaaaaaat atcaagaaat aataagagaa aatatagaaa
     5941 ctaccataaa aatatttgag gaaaaagaag taagaggaga gtttgtatta atagtagagg
     6001 gttttcatgg tgagaagtca aagaaagaaa attatgatgg gcttacagat agggaatatg
     6061 ttttaaaact aattgaaagt ggtatgaaca aaaaagatgc aataaaaata gtttgtaaag
     6121 atagaaagtt aaagaaagat gtggtttata aacaagtttt agatttataa aacctgaatc
     6181 tgaggtgatt ttatggaaat agggaaaatt ataatagaga gtttaaagag ggaagataca
     6241 cataaaatat ttatagaatt agataataag catgaattag aaaaaataat tcctaaaata
     6301 agctgtatga agtctgtagg tgaatgtaaa tatcatgtag taaattgttt tgaacattct
     6361 ataaatgcat taaaagaatt agaaatagta ttaaatgata aggatttttt tccaatacat
     6421 ctaagaaaac atgtgtcaaa ctatttaaac acatatattg aagaaggaat aaataagtta
     6481 catgtattaa agttgggaac atttttacat gatataggta agcctgatag tatgacctta
     6541 gatgaaaccg gaagagttca tttcacaaat catgaaaaaa taggtgctca aataattgac
     6601 aatatgggaa taaagttaga gttatcaaca gagacatata gacttataag taaatatgta
     6661 agatatcata tgatactttt gtctctatat aagaaaaatg atttgagtag gaaagaatta
     6721 attaatgtat ttaacttagt agatgaggac actataggtg taataatgct tgggtatgca
     6781 gatatagtgt ctacagtaaa attattgggc aagagtggag aagtcagtgt gttaaagaca
     6841 tatatggaat atattttaac aaattacttg tataagagta attatacaca tgtataaatt
     6901 tagttaatat aattgagttt tagatgatta attgaagttt gaaatatacg gatttcaaaa
     6961 acataaaaga agttgtcttt gttgtaaaat tatgaaagac aacttttttt atatcaaaaa
     7021 ttgaaatttt attaaggata cttaatggtc tattaattaa taaaccatta gtatttaatt
     7081 tatattacaa tattgatttt gcttgtattg gcgttgatag tataacagag gtcttagtag
     7141 aaccaatttt ctttatagta tcaatcacat tttctagctc gtacatatct tttacaatta
     7201 cttttaaaag taagcaatca tcacctgtta tatggtgaca ttctacaatc ctagggtcct
     7261 ttgcagctga ctcaataaat tctgtatatc cattgcttgg aagagaaata tgaataaatg
     7321 cctttataac tctgcctaat gaatctgggt tgacaatagc tttatatcct tctataacac
     7381 cagactcttc taatcttttg actctttctg aaactgcagg agaagttaaa ccaactattt
     7441 ttcctaagtc cttcatagaa attctcccat catcctgtaa aatttctatg attctgtaat
     7501 ctgtaacatc cataaataaa cagtccccct taaataaaaa taattaatac tataaaactt
     7561 ttttagaatc tatcatttgt gtttttaaat accaaagatt tctagataaa tcaaccttat
     7621 aaatgtgtgg ttttgaaagt cttttgtatt gttcccagat agtacttaat tcatattgta
     7681 catgattttt tatttcaaga acagcttttt tattatattt gcatctgcct tttatataca
     7741 atggtttaag aagctcttta actttgtaat ttgtaaatac ctttgttttc catgtataag
     7801 taggatggaa gatttctaaa ggtttagact catctataac ttcatcatgt aacataatca
     7861 aatctgcctc tgccttattg tcttcgttat atattcttat aactttcttg taaccaggat
     7921 tatttatttt ttctggattc tcagaaatct ttatcttagg ttcaagaact cctttttcac
     7981 aagatgcagc taatttataa actcctccaa gagaaggtga atcgaaagat gtaatgagct
     8041 ttgtacctac tccccatgaa tttatagtag ctccttctgc tttaagtgat gttatggtat
     8101 attcgtctag gtcattagat gcagtgatgc tgatatctga aaagccagcg ttatctaatt
     8161 gttttttagc ttctttagat aaataagcta aatctccaga gtcaagtctt atacccatag
     8221 gtttgtatcc tttttcagaa atatttttga atacttttat tgcattagga actccactgt
     8281 ttaatacatc ataagtatct acaagtagta ggcatttatc tggataaaca tcagcatatg
     8341 cttggaatgc ttcaagttca ttatcaaatt tttgtaccca actgtgagct tgtgtaccaa
     8401 ttataggtat atcaaacatt ttccctgcta gtacgtttgc agttccagca caaccaccaa
     8461 ctacagcagc cctagctcca tatatccctg catctggacc ttgtgcacga cgtaatccaa
     8521 attcaaatac agggtctcct tgagctgcaa agcaaactct agaggcttta gtagcaataa
     8581 gagactgaaa attaactatt gttaacaatg ctgtttcaat aagttgagct tgatataaag
     8641 gtgccttaac agtaattaat ggctcattag ggaacatgat agttccttcc tcaacagcat
     8701 atatatctcc tgtgaattta aattctttta aaaattttaa aaacttgtca gaaaataaat
     8761 ttaagttttt taagtactct aaatcttcat ctgaaaaatg aagattatct atgtattcaa
     8821 caacctgatc aataccacaa actatagtat accctccatc acaagcgttt tttctaaaaa
     8881 acatatcaaa tacaacaatg tcttcatgaa tattcttttc aaaatagcca tttaacatag
     8941 taagctgata taaatcagta agaagtgtta gatttctcat tatttctctc cttaattatt
     9001 attaaatatt aaaaattgta aaaactccca cacttatact aaaacataat atagtacaac
     9061 tttcaagtat aaagtattac ctaactatca taaaattatt taaagaggag gatgattata
     9121 ataggaaaaa tatggtttta tatggtatcg ataggaataa taggaagctt gatttctaaa
     9181 aatatgggtg aattaaataa agttatatta agtgaaacat caaaaggtat agagtttgca
     9241 ataagtctag ctggtatcat ggcactctgg atggggataa tgaatatcgc aaaagaatca
     9301 ggtttaattg aaaagatagg tcaaaaacta aatcctataa tgagaagatt atttccttct
     9361 attccaaagg gtcataaagc aatgtcttat atagttatga atattgcttt aaatatgttg
     9421 ggtgctggaa atggtgcaac agcatttggt ttaaaagcaa tgagtgagct tcaaactttg
     9481 aataacaaga aagatacagc cactaatgat atgattatgt ttatggtaat caatatatca
     9541 tctatacaaa taattccatt tactataatc aaattaagga tggatatggg ggctcaaaat
     9601 ccaagtgaaa taatttttac gactttattt gcaaccataa tatctacagc agtagcaata
     9661 ataacatgta aattttttca aagaagatat agataaaggg gatgtagata catggagata
     9721 ttttcaaact tacttatacc aataataatt ctgtacatag tagtttatgg taaatataaa
     9781 aaaatagatg tgtatgatag ttttgtaaaa ggagcaattg atggattaaa agcagcatgg
     9841 gatatcctac catatataat aggtatattt ttggcaatag gaatattcaa aactggtaaa
     9901 ggtcttgata tgttagaatg gctatttaca ccaatagcaa atatgatgag tataccaaaa
     9961 gaattaatag gtctaataag tgtaaaacct ttatctggaa gtggtgcttt agggatgtac
    10021 agtgaacttg caaatagagt tggaatagga agtttagtag aaaaaatggg tgctacgatt
    10081 gtaggttctt ctgagactat tttttacaca atggcaatat actatggaag tctgaagata
    10141 aaaaatacta gacatacatt atcatgtgct atgattagcc atgttgcagg tgtcatagca
    10201 gctgttttta tttgctatgt aatatttgta taatttgttg acaatatggg aaaaatttta
    10261 taatatagtg attatataga aacaaatagt aatacaaatt acaagctatg aaaagaagag
    10321 taaataaagg ttttttctat cagagagcta ggttaggtga aagctagtgt caaaagcttt
    10381 attgaagatg gtcttggagc tttttatctg agcaagtgtg ctagggtaaa acggttggac
    10441 acgttatagt ctactaaggt atctataaaa tgtttctaca tttcatgtga aacataacta
    10501 ttatagatga attagggtgg taccgtgaat aatctcgccc ctatgttaac tcataggagg
    10561 cgatttttta ttatttaaat gaaagaatca tatgattacg aacgataaaa aattaggagg
    10621 taaataacat gagcaaaccg agtttttatg taacaacacc aatatactat ccaagtggag
    10681 gattacacat aggacatact tattcaacag tagctgcaga tacaatagct agattcaagc
    10741 gtttctgtgg atatgatgta aagttcttaa caggaacaga tgagcatggt gaaaaaattc
    10801 aaaagaaagc catagaacaa ggaatgtcag agatagagta tcttgatgga atgataaaag
    10861 atataaaggc cttatggaat actatggata tatcttatga tgattttata agaactacag
    10921 aaaaaagaca tacagacata atacaaaaga tatttactaa actatatgag caaggtgata
    10981 tatataaagg tgaatatgaa ggtagatact gtactccttg tgagagtttc tggacagaat
    11041 ctcaattact agaaggaaat aaatgccctg actgtggaag agaaacatat ttagtaaaag
    11101 aagaatctta tttctttaga ttatctaaat atgaagacag attaaaagag ttatttaaag
    11161 atgatagttt ctgttttcct gctgctagaa aaaatgaaat ggtggcaaac tttttagaca
    11221 aaggattaga agatttaagc gtaactagaa cgacttttga ctggggtata aaagttcctt
    11281 ttgatgaaaa acatgtaatt tatgtgtggg tagatgcttt atgtaactat ataacagcat
    11341 taggttatat gactgataat gatgaagaat ttaaaaaata ttggcctgcc aatgttcaaa
    11401 tagtaggtaa agaaatagtg agattccata caataatatg gccagcgctt ttaatggctt
    11461 taggtctaga agttccaaaa caagtatttg gacatggatg gatactattt gctgatgata
    11521 aaatgagtaa atcaaaaggt aatgtggttt atccagaacc tataatagaa agatatggaa
    11581 tagacacact taagtacttc ttattaagag agtttgcatt tgggcaagat ggaagttata
    11641 ctcatagaaa ttttgtaacg agaataaatt atgatttagc aaatgattta ggaaacttaa
    11701 taagtagaac tgtagctatg gttgaaaaat acaataatgg tataatacca acagcaaaag
    11761 tttcaactga ttttgatgct gacttaaaag aacaagctgt ttcaactaga gaaaactttg
    11821 aagctgaaat ggataaaatg caattccatg aagcattaga gagtgtatgg aaattagtta
    11881 gaagaactaa caaatatata gatgaaacta tgccttgggc actagctaaa gatgaaacga
    11941 agaaagatga attagacaca gttttatata atttatgtga atctataaga ataatcgcaa
    12001 cactaataaa tcctataatg aatgagactg ctaataaaat atatgaacat ataggaataa
    12061 aaggtcaaga tgatataact acatgggaaa gtacaaaaac atttggtctt attggagaaa
    12121 atgtaaaagt atttaaaggt gaacctttat tcccaagatt agatgtagaa aaagagatag
    12181 aagaattaac taaaatgttc tctgggaaac ctccagtaga agaaaaacct ttagagcata
    12241 aagaagaaat tactattgat gatttagaca aaatagagct tagagtaggg aaaataataa
    12301 gctgtgaaaa acatccaaaa gcaaataaat tattagtatc acaagttaag attggaccag
    12361 aaacaagaca aatagtatca ggaatagctg aatactataa gccagaggat ttagtaggaa
    12421 aagaggttac agtggtatgt aacttaaaac cagttaaatt aagaggtgta gaatctcaag
    12481 gtatgatatt agcagcaggt gatgatggag acccatatgt tttaccattt acacaagggg
    12541 ctaaagatgg atgcgaggtt cgttagtagg taattaaata gtaaggagat taaagatgtt
    12601 atttgactca catgcacatt taaatgatga aagttttgat gaagatagag atgaacttat
    12661 tggttcatta aaagataaag gtgtagattt ggttgtaaat ccaggagcag atatagagac
    12721 gtcgataaca gcaattgaac ttgcgaaaaa atacgacttt atatattcag cagttggggt
    12781 acatccacat gatgtatcaa aactagatga tacagcaata gaaactctaa gaaaacttgc
    12841 aactgagaat gaaaaagttg tagctattgg agagattgga ttagattatt attatgatta
    12901 ttctccaaga gaagaacaaa aagagtggtt taagaagcaa attgaattag ctaatgagct
    12961 aaaacttcca ataataatac atgatagaga tgcacatgga gatacttttg agataataaa
    13021 gaatactaaa aatcctgaaa taggatgtgt acttcattgt tatagtggaa atgtagagtt
    13081 agctagagaa tatgttaaga tgggttgtta tatatcaatt ccaggcacgg ttacttttaa
    13141 gaataataaa aaaactagag aagtagtaag agaaatacct ctagaaagat tattcataga
    13201 gactgattca ccatatatgt caccagagcc acatagagga aagagaaaca atccttctca
    13261 agtatctttt gttgctgata agatagccca ggaaaaaggg atatgttatg aagaagtatg
    13321 tagagttaca aaagaaaatg caaagaaatt ttttaatata aaatagacaa attaaatctt
    13381 ataatgatat atataataag tcattataag tataggatga gagtgcctca aaatgagttt
    13441 tttagttcat gaggcactct ttttttatga aatcaaatat attttcatca tttcaacaat
    13501 gaaaaagaag tttatcatat tttgagacag tttgttttta tttgtttaat tcatatttta
    13561 aaatatattt tcttaacatt gttaaaaaag aatttacaag atattcatta gaattaactc
    13621 aactctaata ttcacttaac ttaagtggtt tattatatta ttaaagagaa gtaaacgaaa
    13681 ggtagattaa ggaggctaaa ttttgtatac agttgatagt actaagatat tgataaatat
    13741 gaacttagag aaactagaaa agatatactc aatgataaaa gatacatcaa gtatttctat
    13801 aattaaagaa cctaacttgg ctacagtaat ggtaagagct aatgaaagtg taaaaaatac
    13861 aacatttaat ttaggagaga tattagttac tgaatgtagt gtaaaggtag atgaaagctt
    13921 gggttacgga atagtttcag aaaacaataa taagaaagcc atttacttag cagttataga
    13981 tgcagtttta catagcaata acagtaaatt tgatgagtta aagaattaca taaataaaag
    14041 tgtatatgaa gagaacttaa ggtatgaaca agagattatt gatgaattta gcttgattaa
    14101 taaaacgaaa gttcaattca atgctatgga ttaaatagga ggagaatcat ggtaaattat
    14161 acacaagaaa tatatagaaa gatgctagat agtgcttcgt ttcctgggaa attaaataat
    14221 atagataata taaatataga aaataataca aaactattga atggagcaat tggtatagca
    14281 atcactttat tagaccaaga agttaccttc tatatagaaa attatactca aaaagatata
    14341 aaaaatataa gagcgcttac agtgtcaaat caaatagatt atagaaaatc agattatata
    14401 tttttagata ttaattcaga agtggatata ttacagatta aaataggttc ttttgaatat
    14461 cctgatgaaa gtgcaactat agtacatcaa gtaaatgatt tatcaacgta ctctcaaact
    14521 aagtatataa agcttgattt gagtggtcct ggtataaaat ctaaaaatag tatatatata
    14581 aatggaatta gagaagaatt tatagaaaga ttatctacca taaataaaga ttatccaata
    14641 ggaatagatt tgattttagt agataaaaaa ggagaaattg catttatacc aaggtcatca
    14701 aaattaagtt gggaggtatt gtagaaatgg cttatgtagc agttaaaggt ggagaagaag
    14761 ccatattaca ggctaaaaat atacttgagt tttatagggt aaaaggagat agcttgccta
    14821 tagatgtaaa gcagattgag gaacaaatgt cttatgctgt agacaaagta atgtctgaag
    14881 gaagtttata caacaaggaa ctatcagcat tggctataaa acaagctcaa ggtgatatat
    14941 tagaagcctc tttctatgta agagctttaa ggtcaacaat gcctagaatt acatattcag
    15001 aacccataaa tacagaagaa atgagaataa aaagaagaat atcagctact tttaaagata
    15061 taccaggagg tcagtatctt ggggcaacta gagactatga aaatagaata ttaaatgtag
    15121 aattattaga agaagatgaa aaaaagaata gagattttag agatagtttt ataaatgaat
    15181 tagaaacagt agatgaaatc cctgaattta caaaaatatc taatatattt agaaaagaaa
    15241 atttattaca agaagaagtt tgtgaaaaca aattggaaga agttattgat gatattacaa
    15301 gaaatagttt aagatttcca gtttctaggt cagcaaagtt acaggcattg actagaggtg
    15361 aaagtggagt agtgtcaggt tttgcttatg ctattgtaag aggttttggt gatgaacatc
    15421 catatataag tgaacttaga actggtaatg tatttataaa agtaaaacat cccatctatg
    15481 aagatgaaga aataataata ggtgatatgc tggttactga atgtgaaata gtttcaaaag
    15541 catcacaaaa taagactgaa aattctaaaa ctaattcgga agataaaaat aagatgtctt
    15601 taggatatgg tctttgtatg gggaataatg agaataaggc tatatctatg gccatactag
    15661 acaaatcttt ggagccaaat agtgaaaaac tagcacaaga tgaagaattt gtattgcttc
    15721 atatagatgg aatagattca ttaggattta tatctcactt taagcttcct cactatgtag
    15781 attttaaagc ggatgtagaa agaataaaat agtttgtgga ggtaagttta ggtgatatat
    15841 aactatggtt ttttagacga aaacacaaaa aaagaaataa gaagaaagat attaaaagca
    15901 atatcaatac ctggttatca agttcccttt ggttcaagag agttgcctat agctaaggga
    15961 tggggtactg gaggtttaca attaacacta tcattaattg gtaaaaatga tgtagtaaaa
    16021 gtaattgacc aaggaagtga tgattctaca aatgcttgta atataaggag ctttattgca
    16081 agtgtaagta atgtagaaac cacaaaagat acattagaag ctacattgat tcaaacgaga
    16141 catagaatac cagaagaaaa acttaaatct aatcaaatac taatatttca agtaccaata
    16201 ccagaaaccc ttagaatagt agaacctagt gaagtagaaa caagaagaat gcattcagaa
    16261 gaagattata gtaggatgtg ggtatatctt tatgaagata tagttagatt taatgatata
    16321 tctatagcag ttgaatatcc atgtaaagta aatgatagat atttaatgaa tccatcacct
    16381 atacctagat ttgatataaa aaaattaaat atgtcagaca atttattctt gtttggtgca
    16441 ggtagagaaa aaagaattta tgctattcct ccatatacaa aggtagaacc tttagagttt
    16501 gaggattata agtttgaaga ggaaaagttt gaaggaaaat actgtagttt atgtaacagt
    16561 acaaatacat ttttagatga agtatatgac agtgacacta atgaaaagta ctatagttgc
    16621 tcagatacaa gttattgtga aaaagttaga ttaaaaaata acagtataga tgtgacaata
    16681 ggaggtactt ggaatgagta aaataaatgt ctctaaaaga ttagaaaata tagacaagca
    16741 agtattaata ggaagaaata tcaatcttat ttatggagat gggtgtgaaa agtgttttga
    16801 aagcacagga tatgaaagca ataatatatg taagtattgt ggttctatag ttgcatgtaa
    16861 taatgtaaat ttagaacttt atgaaggaga agtccttgga attgtaggtg aatctggttc
    16921 aggaaaaagc actttattaa aaatactgtt tttccaagaa catgcaaata gtggagaagt
    16981 atatatatcc cattacaata aagacacaaa tatactagag ctttcagacc aaaaaaagag
    17041 atacataaag aatcatttta tgggtattgt gtatcaaaat ccccaccttg gtttaaattt
    17101 agactttagt agtggtggta atatagcaga aaaattattg atggcaaatt tatataatgt
    17161 tgaaaaaata agagatagag gcaaagaact tttaataaaa actaatatac ctgtagaaag
    17221 aatagatcat aaaccaaaat actttagtgg gggaatgcaa caacgagttc aaatagcaaa
    17281 agctctttca aataaccctc caatattgct attagatgag gttacaacag gtttagatgt
    17341 ttctgtgcaa gcggaagtat tggatttaat aagagaaatc caaagagaac ttaagatttc
    17401 tatgatagtt gtatcccatg actttgatgt tataaagatg cttgctgata ggacaattgt
    17461 aatgaaaaat ggaagtgtga ttgagtctgg tcttacagac caaataatgg aggacccaca
    17521 acacccatat actcagcaac ttataaattc attgctataa atatcgtttg taaataagca
    17581 aaatgggggt ttttaataaa atgaaagaaa ctatacttga agtaaataat tttagtaaag
    17641 actttgagtt acatgcattg aacaagacta taacggcatg tagtaatata agttttgaag
    17701 tttcaaaagg tgagttttta ggaataatag gtaaatctgg agcaggtaag tctaccatat
    17761 taaaaagtat ctataaaaca tatataccaa caactggaga aataattttt aattcagaaa
    17821 tatatggtaa tgtagacctg gctaaaattg gagaccgaga aatgataaat cttagaaaga
    17881 aagaaatagg atatgtatca cagtttttaa agacacttcc acgaataact gcaattgaac
    17941 tggtagtaca ttctttaatt gagagtgggt ttgaaaaaga ttgttcgtat gatatggcaa
    18001 aagatatact taaccaattt gaaataaagg aaaatttatg ggatgcgtat ccaaataatt
    18061 tttctggtgg agaaaaatta agattaaatt tagctcaagc tatggttaaa aagcctagac
    18121 ttttgttact tgatgaacca actgcatcac ttgataatca atctaaaata tatgtaaaag
    18181 aaaaaatgtt ggaacttaaa agtcaaggaa ctacaatgat aggaattttt catgatatag
    18241 agtttatgga aactgttata gataagacct ttactatggc aaaagggact ataagtgaaa
    18301 gtggggtagc ctagatgata aaactaattc ataatgcaaa tattgtatta gaagatatga
    18361 ttttagaaaa tggtcattta atcatagagg gagatacaat aaaaaaagta tcaaatgata
    18421 ggattgatta cgcatttgac tttaatgaga tagatgaaat aatagatgca aaaaacttat
    18481 atgtaatacc tggtattata gatattcaca gtgatgccat tgaaaaggaa atagagccaa
    18541 gaccaagtac attattacca tttaatatgg cattttatga actagataaa aaactacctg
    18601 caaatggaat aactacagtt tatcattcta tatctttagg ggatggagta ggagttagaa
    18661 gtattgataa ctctttaaag atgattaaaa atattgattc atataaaaat atagatagta
    18721 agagtataaa tcataaagtt catttaagat atgaagtgtt gtattatgaa ggtttagaaa
    18781 aggttttaga actattagat gaaaataaaa tagactactt atcaataatg gaccattctc
    18841 caggacaagg tcagtataca aatccaacat tttataaaga gtatgctact aaagtgtggg
    18901 gagttacaga aaattatgta gatacatggt tagatgattt agtaaatctg catgataatt
    18961 tagattggaa taaaatagct aatattatag gtatagccaa aactaaaaat ataaatgttg
    19021 catcacatga tgatgataca ttagaaaaaa tggatttcat aaaaaatata ggcataaatg
    19081 tatctgaatt tcctataact cttgaagttg caaaacattc aaaaaaattg ggtgttctaa
    19141 cttgtttagg tgcaccgaat attgtaagag gtaaatctca taataacaat ttaaaagcaa
    19201 tggatgctat attggaagat tgttgtgaca ttgtgtgttc ggattatctt ccatcagcta
    19261 tgataaagtc tatgtgtata gttgcagaaa gaataaataa tttaaataaa gcagtcagtt
    19321 tatttacctc aaatcctgct aaagcagttg gtatatatga tgaaagaggt agtataaaag
    19381 aaaataaaaa agcagattta gttttagtag atattgatag tgaatatcca aaggttttaa
    19441 atactattgt aaatggaaaa acagtttata aaagggaggt ataaaaattg ctaacagagt
    19501 tacattgtca tacaagtata tcagataata attttactac tgaagaaata ataaaaaaag
    19561 cagcagaaaa gaatataaat atcttagcaa taacaaatca tgacacatta tctggattgg
    19621 atgaagctct tgtgcttgga gagcattatg gaataacaat aataccaggt atagaaattt
    19681 cagcctatga ttataaaaat agaaaaagag ttcatattct tggatataac atagatttaa
    19741 acagtcaaga aataaaaaat ttgtgcaatc caatggtatg tgacagacat aaagcatctg
    19801 ttaagatggt aaataaaatt atagatttag gatacaaaat atctttggaa gatgtaaaga
    19861 aatattcatc aaagactgga atatttaaac aacatataat gaaagctctt atagataaag
    19921 gatatacaga taatatatat tcaagtctat ataaagaatt gtttcataga ggaaacggca
    19981 aggtatatgt atctttaaaa tatgtggatt acagagatgc aataaaagct ataaaaaatt
    20041 ctggtggaat ttgtgtgtta gctcacccgg gtcaaatgga taattttagc gctattgaag
    20101 agatggtaaa agtaggatta gatggaatag aagtatatca tccatctcac aacaaagaac
    20161 tagaaaaaga gtcgctttct tatgctaaga agtataatct agtggtcaca ggaggctcag
    20221 attaccacgg attttattct caacataatt atgaacttgg aagcaagagc ttaaataaag
    20281 aagatttatt taagtttagg ttagctttag gaggggtatt ataaatgtct tttttacaag
    20341 taaaaaatgt aggtaaaagt tatggacaag ttaaggtgtt aaaagacata tctattgata
    20401 ttgaaaaagg agagtttatt tgtttactag gtccaagtgg ttgtggaaaa agtacactgc
    20461 ttagaataat tgcagggctt gaggataaac atggtggaaa aataataata aatgataagg
    20521 atatgactaa ttcaccacct gaaagtagaa attttggtat agtttttcaa tcatatgcat
    20581 tatttccaaa tatgaatgtt tacaaaaaca tagcctttgg actagaaaat aaaaacataa
    20641 gtaaatctaa tattgataaa aaagttaaag aagttctaga cgttgtagaa ttaagtggat
    20701 atgagaaaaa atatccatca caattatcag gtggacaaca acaaagggtt gcattagctc
    20761 gtgcgattgc tttagagcca gactttttac ttcttgatga acctttatct gcattagatg
    20821 caaaagtaag acttaaactt agggagcaga ttagaagttt acatagaaaa ctaggcatta
    20881 caactataat ggtaacgcat gaccaagaag aagctctttg tttagctgat aagatggttg
    20941 ttatgaatag aggagaaata atacaagttg gaacgccaaa ggaggtatat aaaaatccag
    21001 aaacaccttt tgtagctgat tttataggaa caataaattt tatagatgat ggtattaata
    21061 agatagctat tagaccagaa gatataaaag tagagtctaa tagagattct aaagataagg
    21121 atataaaagt gggagaaatt ttagatatcg aatttagagg atttaactat agaattacag
    21181 ttgagtatcg ttctaagcaa atgaaattag atgtagtttc caaagttgca gaacaaatga
    21241 aactgtgcat aggttctaaa ataaacttta agatacctaa agaaggaatt gtacaatata
    21301 aatcagaggg atgtgcttaa tagctagggg tgaataaatg ataacatcaa aaaataaaag
    21361 aagaataagt gatagcacaa tacaaaaata tttcttgatt ttttcggtaa ttgtactaat
    21421 agtatttata ttatttccat tagtatcatt attaaaaaat gcatttatgg atacaagtgg
    21481 aaattttata ggaatttcaa actttagtaa atatgttgaa aatccaagtc ttatagcatc
    21541 atttaaaaat tcaatttttg tatctacagt ctcatcttta atatcactat gtttagcatt
    21601 tatatattca tacgcaataa caagaacaaa tataaagttt aaagattttt ttaaaactat
    21661 gggaatgttt ccactttttg caccaactat gttatatggt atttcactta tatacctttt
    21721 tggaaataag ggaatcttta caaccatggg tctggaagta ccactatatg gtccattagg
    21781 aataattatt tctcagattc tatttacatt tccacaagct tttttaatat tatctgtagc
    21841 actttcgatg gctgattata gactttatga agcagcagat agcttgggtg ctagtgaatt
    21901 taagaaattt tgcaaaataa caattcctgg tataaagtac tcattactta gttcattctt
    21961 tgtatccttt atacttgcat ttacagattt tggagcagct aaggtagtcg gaggaaacta
    22021 caatgtactg gctactgaca tatataaaca agtagttgga caatttaaca taccaatggg
    22081 agctactgtt tctatggtaa tgttaattcc tgtactaata gcatttacat tagataagat
    22141 ggcaagtaag aaacaaggta tgaccataac tgcaaagtct atgccatata agattaagga
    22201 aaataagttt agagatagag tatttttaat agtatgtagt tttataacat tgctaatttt
    22261 tattttaata ttcgtttcag ttataggttc agttataaag ttatggccat ataatttaag
    22321 ctttacaatg gaacactata catttaaagg tattttggga agtggaataa aaacatactt
    22381 aagcagtttg aaaatatcaa ctttaacagc aatatttgga actatatttg tattttttag
    22441 tgcttatata atagaaaagg tggataagtt tcctaaatta agacaaacag cttacctttt
    22501 atcaatgata ccaatggcct tacctggctt agttttaggt atatcatata taactttttt
    22561 taactcagca agtaatccat taaatttttt atatggaagt acagcaatat tagttatagt
    22621 aaatgtagtt catttttatt cagtttgttt tataacctca aattcatctt taaaaatgtt
    22681 ggataaagag tatgaattag ttgcaaaatc tataaatatt ccattctata aagtattttt
    22741 taatataaca gttccgatgt ctataacatc aatacttgaa atagtagtat attattttgt
    22801 aaattcaatg gttactgttt cagcattaat atttttatat atacctcaaa ctcagactgc
    22861 atcaatatca atactaaagt tagatgagat aggatatata ggaccatcag ctgctatggc
    22921 agtattagtg ctattaacaa atataatagt aagacttttg tacgaatttg taacaaagaa
    22981 attaaaaaat aacacacaaa aatggcagca aaaagatgtt gcatagggga ggaaaaaatg
    23041 aaatttaaaa agctagtttc tttaggagta ttatcaacac ttttattaag tgtaataaca
    23101 ggatgtagta ctgattcatc aggttcagat tcaaagtctg atagtaagac aaaaggggat
    23161 ttaacagttt acacagcaat tgaagaagat agtatagagc catatttagc tacatttaaa
    23221 gagaaatatc cagatattaa attaaatatt gttagggcat caacaggaga tataacagca
    23281 agattattag cagaaaagga taatcctcaa gcagatgttg tatggggagt tgcagccaca
    23341 agcttattgg ttgcagatga ccaggggatg ttagaaccat atgctccaaa aggttctgaa
    23401 gaaatagagt ctgcatttaa agatgataaa gaagttcctt cttgggttgg gatagatgct
    23461 tggatgacag gtataacagt aaacactaaa gaattaagtg ataagaacat cccagttcct
    23521 agttcttacg aagatttaat aaagccagaa tataaaggtc ttatatctat gcctaatcca
    23581 tcttcttctg gtacagggta cttaacagta tcagccctta tacaaattat gggggaagaa
    23641 aaagcatggc agtatatgga taagttgcat gagaatattg gtgtatacac tcaatctggt
    23701 tcagctccag ctacatcagc agcttcagga gaatatccta ttggtatatc ttttggatat
    23761 agaggtataa aattaaaaga ggaaggctat ccagtagaag tagtatttcc aaaagaaggt
    23821 tctggatggg atatagaagc aaatgctctt gtgaaaaaag ataatataaa agaagcatca
    23881 aaagtatttt tagattgggc tataagtaaa gatgcaatga atgaatactc taagaactat
    23941 gcagttacta ctatttctac aggaaatcca ataccagaag gattccctaa aaagccatta
    24001 gagcaaatga tagataatga tttaaaatca gcagcaaaaa atagagaaga tatactaaat
    24061 aaatggatat caaaatatga tggtaagaca gaaaaagaga gttaagtaaa actatacaaa
    24121 tggagtaaaa agaaaaaatt tttttaagta gattttatta taataatata agttgagaat
    24181 aatataggtc aagaaaagtg aatattctat aaaaatgtat agagtaagta taccattcag
    24241 tttgaatggt atatttttta tttttagaga ttaatttaga aatagatata ctacagttta
    24301 aaatagtata ttttaatttc attattaaag tatagatgtt atatataaag taaataagat
    24361 attaaataat tataaactaa agtaaataaa atattgagcg attattaaaa agatatatat
    24421 atatatttta atttgagcag tttgttatat attataaaaa aagagaaaaa taaaaggtaa
    24481 attgtttctg gataacttaa aattggaagt aagaatattt aactagagaa gaattattta
    24541 agttcaggtt agctctagga gtagctgatt ttataggagc aatagatttt atggatgatg
    24601 atataaacat gatagcaatt agaccagaag atataagggt agaatttaat agagatttca
    24661 aagaaattat agacctcaaa ctcagaccgt atcgatataa aaactaaagc tagatgatat
    24721 agtgttgtat ataggaccat cagctgttat ggaagtatta gtgcttttaa caaatataat
    24781 agtaaaactt ttgtatgaat tgataataaa aaaattaaaa accaagacat aaaaatggca
    24841 acaaaaagaa gttgaataag ggaggtaaaa atgaaattta agaagttagt ctctttggga
    24901 atattatcaa cacttttatt gagtgtaata actggatgta gtaatgtttc atcaggttca
    24961 aacttaaagt ctaataataa gacaaaagga gagttaacag tttatacagc aattgaggaa
    25021 gatagtatag atacatactt agctacattt aaagagaaat atccagatat taaattgaat
    25081 gtacttagat tatcagtagg aggtataaca gcaagattat tagcagaaaa agataatcct
    25141 aaagcagatg tgttatgggg tgttgcagct acaagtttga taattttaga tgacaaagaa
    25201 atgctagagc catacgctcc gaaaggcttt gataaggtaa atcctgcatt taaagatgat
    25261 aagaaagttc cttcttgggt tggaatagat gcttggatga ctggtataac agtaaatact
    25321 aaagaattaa aagctaaaaa catatcagct cctaattctt atgaagattt aataaagcca
    25381 gaatataaag atcttatatc tatgcctagt ccagcttctt caggaacagg atatttaaca
    25441 gtatcagaac tcatacaaat tatgggtgaa gaaaaagcat ggcagtatat ggataaattg
    25501 cataaaaata ttggtgtata cactcagtct ggttcagcgc cagctatatt agcagcttca
    25561 ggagaatatc ccattggtat atcttttgga aatagaggta taaagttaaa aggtgaaggt
    25621 tatccagtag aagtagtatt tccaaaagaa ggttctggat gggagataga atcaaatgca
    25681 cttgtgaaaa aagataatat aaaagaagca tcaaaagtat ttttagattg ggctataagt
    25741 aaagatgcaa tgaatgagta ttctaagaat tttgcagtta ctacaattga cacgggaaat
    25801 ccggtgccag aaggatttcc aaagaatcca ttagaacaga tgatagataa tgatttaaaa
    25861 tcagcagcaa gaaatagaga ggctatacta aataaatgga tagaaaaata tgatggaaag
    25921 acagaaaaag agagctaagc cttacaataa aatttagtac aaaaagcgcc tagaatataa
    25981 gttttacatc taggctcttt tgatatattt atttaaaatg catatttcta tataaaaata
    26041 aatagtttag taccttgatt aaattaaata aaaaccttaa aataaagaga tgtaaagaaa
    26101 aactaaaaac gaataaaagg tgaatttata tgataaaaga aataatagta gtagagggta
    26161 gagatgacgt tacagcagtt aagagagcaa tagatgcaga acttataact acaggtggat
    26221 ttggatttcc aaaaggtgta atgcagcgta taaaagctgc taatgaaaga cgtggtgtta
    26281 taatatttac agacccagat tttgcaggtg aaaaaataag aaaaaaaata gcatcagaag
    26341 ttccaggatg taaacatgca tttctaccaa gagaggaagc taaaaaggat ggagatatag
    26401 gtattgagaa tgctactcct caaagtataa tagcagctct gaataaagtt agaactgaaa
    26461 atacagaaaa aagagatgaa tttaagcaag ttgacttaat tagaaatggt cttataggta
    26521 atgaagaagc atcacttaga agagatgcac ttggaaaaat acttggaatt ggatatggaa
    26581 atgcaaaaca atttttaaat agattgaata attatggagt tgaaagagaa gaatttatag
    26641 atgcattaaa acaaatctaa acgtaatgtt taatgtatga aaaatatgta tataaaataa
    26701 attgtggaat tacggttaat ttaagataaa ttatttataa tatatacctt attttaaatc
    26761 aatgtaaata catataaata agtataatta ataaaattaa atatcaaaat aagttaaaaa
    26821 ggagtaaata atggatagac tatcttcaca taatgcaaca aaggaagtag tacaaaaata
    26881 taattttaaa ttttcaaagt ctttaggtca aaatttcttg atagatagta atataattga
    26941 taaaatctta tctggagcta gaatcacaag aggagataat atcatagaag taggacctgg
    27001 tataggaact cttactcgtg agatgggtaa aatagcagaa aaagtagtgg ctatagaaat
    27061 cgatagaaat ttaattccta tattgaaaga taccttgtca gatttagata atacagaggt
    27121 tgtaaatcaa gatatactaa aagtagacat acaggaatta gtaaaagata aattaaatgg
    27181 tggaccagtt aagcttgtag caaatcttcc atattatata actactccaa tagttatgaa
    27241 atttttagag gaagacatac ctgtaacaga catagtagtc atggttcaaa aagaagttgc
    27301 agatagaatg aatgctattc cgggtactaa agattatggg gcattatcta tagctgttca
    27361 atactattgt gatacagaga ttgtggcaaa agctccaaga catatgttta taccacaacc
    27421 aaatgtagat tccacagtta taggtttaca tgtaagagat aaaagaaaat atgatgttca
    27481 taatgaagac atatttttta aaacagtaaa agcatccttt ggacaaagaa gaaaaactct
    27541 tcttaattcg ttgggaggtc ttggttttct taataaagat gaaataagag aaatattaaa
    27601 agaagctaat atagacgaaa aaagaagggg tgaaactctt agtatagaag agttttcagt
    27661 tctttcaaat ataataaata caaaagtatc ttctaaatag aaggtacttt ttttaatttt
    27721 gttaacatat attgtttaga gaaactgtgt taagggggga ttgcagtggg ttctaaaaga
    27781 aagtttaaaa gagactatat aatactagaa gctaaagaca taaactttag atacaaagaa
    27841 cgagtactgc caaaagcttt tgcaaaagtt gaagtaaatg atgaaaaagc aattattgca
    27901 ttatatgtag aaaatctaaa atatgttaaa gatgggtata aagcagtagc aataaaagat
    27961 gaccatggat taatagactt aggaaatata attttaaatg aacaaggaag aggtgaattt
    28021 gttttagatt tagaagattc tgatgatgag ataaaaggta ttgcattatt gtatgaaaga
    28081 aatatacctt taattggatt taagggaaat aagcttgaaa attatgagga catattattt
    28141 caagaggtag aggaagaaga atatgaagaa tatgaagaat atgaagagtt ggaagaagac
    28201 gaagagatag aggataatga ggaaagtgat agagacgaag atagtgaaga agaatatatt
    28261 atagaagaat atgatgaaga tgagtatgaa gactattggg aagatgatga agatattgaa
    28321 gaaggagagt taaaagcaag aacggaagta gaagattctg gtgatgagga aatagaagag
    28381 ttcaataatt ataaggaaat agaaaattat aatagggtta aagtacagga agaatatata
    28441 gacatagaat atgatgagga agaagaggaa gaagaagaag tttttgaagt aaatagagaa
    28501 gaagataggt ttgtttcctc aaaacctact aaatcaaaga aagttcaaaa agatcagaat
    28561 aatattgaaa aaataaaaag agctaatgga gtaagtcaag gagagagtgg aaataaacaa
    28621 accagtacat tacttatgcc aagacaaata aaaaaaggtt tgaaattatt taaagaagta
    28681 aaaccttttg taacagatta tatagataag acaagatggt ggaaaataga gataaatcct
    28741 acaacactgt gtggatatac aatgccttat ttaggatatt taaacgtatt gaattacact
    28801 atgtatagtg atgcagtttt acattcatat aaatacagac actatttatt tggtgttcag
    28861 tatgatgaat ataataagag aaaacattat atttatgcta tcccaggaaa tagaaatgaa
    28921 cagccagaca atggaagcac aggattttta agatatcaag cttgtgataa tagaacaaat
    28981 agtttaggat attggctatg ttttatagat tctagacaaa gaaatatagt taggtaaaaa
    29041 taataaaaat gaaaaaattt agactcaatt cttattttac tgaaaaaatt aatgaaatgt
    29101 agtacaaaac tttaaaaact attgataata tttaaatatt acactacaat atatcttgaa
    29161 atacctatta tattatggag gaaaattatg ataaacgagc aaagaatttt aaatgagttt
    29221 cttgaattag tacagataga tagtttatct ttaaaagagg ggaatgttgc taaagtatta
    29281 gttaaaaaat tagaagaaat aggttgttca gtagtaatag acaatgctgg tgaaaaagca
    29341 aatggagaaa ctggtaatat aatagctaca ttaaaaggaa ataaagaagg aaagaaaata
    29401 ttatttagtt cacatatgga tacagtaact ccaggtatag gggtaaaacc tatagtggat
    29461 gaagcaaatg gaataataaa aagtgatggt actacaatat taggttctga tgataaagca
    29521 ggtatagcag ctatattaga aggcttaaga tatataaatg aaaataacat agaacatact
    29581 gatatacaag ttgtattctc tatatgtgag gaatgtggat tagttggagc taaaaattta
    29641 gactatggaa aaatagattc tgaatatgca tttatattag atagtggtgg ttctccaggt
    29701 gaaataatag taaaagctcc tgcacaagat gtaataaatg ttaagatatt aggaaagact
    29761 gctcatgcag gtcttgaacc tgaagctgga ataagtgcta taatggtagc tgccagagca
    29821 atagaaaata tgaatttact tagaatagac gaagaaacaa ctgctaacat aggaattata
    29881 aacggtggaa cagctacaaa tatagtaaca ggagaagtta atatagttgc agaagccaga
    29941 agtctaaaag aaaataaatt agatgttcaa actaaacaca tggtagagac ttttgaaaaa
    30001 gctgcaaaag attttggagc tcaaattgag atagatgtca atagagcata tacaccaata
    30061 gatgttagtg aggattctga aataataaaa cttgcaaaga aggctttttc aaacttaggt
    30121 atagaaggtc atactgaatc tacaggagga ggtagcgata caaatatatt aagtaagaat
    30181 ggtatcgaag ctataacttt aggaattggt atgaaaaatg ctcatacact aagtgaacat
    30241 atagctataa aagacttata tgactctgct aaaatggtag tggagataat aaaggaagca
    30301 taaatttaaa taatagtgta taatatatat atcgcctagc ttaagctagg tgatttttca
    30361 ttttatagaa gattgataat ctctattgaa agcaaagagt atacatttgc atataagaga
    30421 gtgtttaatt tatgttcaga tgtaggttct taataatatt tagattgttt cataagggag
    30481 gaataaatat ggaaacaagg tatgaagaag caaataaaat aacaatacaa tcaatacttt
    30541 ggaatgtagt attaacaata attaaggtta tagctggtgt tattggtaat tctagcgcta
    30601 tgattgcaga tggattacat tcagcatcag atattataag ttctatagga gtactaatag
    30661 gcaattatgt atcttctaga cctggagata gagagcacaa ttatggtcat gagaaagcag
    30721 agacattagt atcatttgtg ttatcaatat tgcttatttt cgtttctata acaataggta
    30781 tagaggcaat taaatcttta tttaacttag atgcattaag tgttccatca atattaccat
    30841 tagtagtatc agttatttca atcctgataa aagagtatca atatagaata acaataaaag
    30901 ttgctaaaaa gataaattca ccagccttaa aagctgatgc ttggcatcat aggtcagatg
    30961 ctttatcttc agtagctgct tttataggta taggtggttc tattttaggg tttaagccac
    31021 tagacccaat tgcgtcaatt gtagtggcta tatttgtagc taaagtagga attagtatac
    31081 ttataagttc tgttaatgaa ttgatggatg tttcagttga cgaagaagag ataaaagaat
    31141 taaaatttat tgtagcagat acagaaggtg ttaaaaatct tggtgatata aagactagaa
    31201 aacatggagc tatggcatat gtagatttga ctatatgtgt ggatgaaaat ttgacagtta
    31261 agcaaggtca tgatattgct accaagcttg aaaaacatat aataaaacat atggaatttg
    31321 taaaaggcat aacagttcac gttgaaccat gtactaattg ccaaggaaat aagtgtaata
    31381 attaattaaa acttaaaaaa aagtgtaaaa agttatataa ttgttttatg attataaact
    31441 ctaggggtgg ttaaatgagc aagaaaagaa aactaaagaa aaaagctgca atattaatag
    31501 gagtagttgt tttctttata atatacttta tatcctttag aataggaata aatatggaaa
    31561 aggataaaag tgaagtgcca aagaaggtag aaaaagaaga agaagataat aggtctttaa
    31621 ggactcaaat aaaagaaaag aaaaaggttt atatatcaga caaaaatata gaaaacataa
    31681 aaattgaaga agatatgtgg gatgaaataa agttattttt ctcagatttt aagaaagtaa
    31741 gaaatactac ttcttcttac gaagcagttt atgaaggtta ttctgatgat ggagttaaat
    31801 tttctactga ccttaacttt tttagagtat atactgtaaa taaagaagaa tattataaag
    31861 taccagttag ttctaaggat gcttttaaga aacttttaga tgagagtata tatacttcat
    31921 ttgacctttt aagtcattat aaagattgga aagaggttac tataactcat ggaaaagaaa
    31981 ttaaaaaagt tcatagatgg aaatttgatg atttagcaca taagatgatt tcaaagaggg
    32041 ttgtaggaaa agttcaacct gagaaaagta aggaaagaag tgactataat tttactataa
    32101 atataaaagg tgataatttt gatgtaaaag ttgatacaat gggaaaagat tatgtaaaag
    32161 ttacttctaa gaaaaatgta tcttactatg aggttcatac aagtttattt gattacctta
    32221 aaaatgagat atttgaaatt ggaaagaaat aagactttaa gaaggctatc aaattagata
    32281 tttgatagcc taaattattt ttatatatta tttttcaacc aacaagtctg caaccttata
    32341 aattggacca gcaccagcag ttattactaa atcattatcc ttaacatttt cacgtaagta
    32401 ttttactata tcttcaaatt cttttatata gatggcatct acattgtttt gatataattt
    32461 ctcaaccaag tccttagaat gtatatctcc agggtctttt tccctagcag catatatatc
    32521 tgttatgata actttatcag cagcatagaa tgcctctgaa aattcaccta aaagagattt
    32581 tgttctagta taagtatgtg gctggaagat acaccataaa gttgattttt ttaatttttt
    32641 ggcagcagct aaagttgctt ttaactcagt tggatgatgt gcataatcat ctactactaa
    32701 agcattctta taataccctt tagtttcaaa tcttcttccg acacctttat aaatttttat
    32761 attttttctt atagtctcta agtcaatacc agaaacaaga gctgccatta tagcagaaga
    32821 agcattgtat atgttatgaa gaccatatac agaaagttca aattctccta aatctcttcc
    32881 ttcatattct attctaaata ttccatgacc actattgtca aagtgaatat cttttattac
    32941 agcatcattg tcagaatctc taccgtattt tataatagta gctttaacat catacaatat
    33001 gtcatcaata ttttcatcat cgccatttat tatgaaatag ccttctgggg gtaataattt
    33061 tccaaatttg ttaaaagaag ccttgatttc atctattcca gagaaataat caagatggtc
    33121 ttcttcaaca tttaaaacta tagatatctt tgggttaaaa ttaaggaaac tatctacata
    33181 ttcacatgct tcagttataa agtggttgga attacctatc ttaacattac cacctatcat
    33241 acttaagtta ccaccaacta gtatagttgg gtctaagtct gcatattcaa agattgtaga
    33301 taacatagat gttgtagaag tttttccatg tgttccagaa actgctatag aatttttata
    33361 ttccctcatg atttggccta aaaaagtcgc tctgttcatt accaatttat ttttttcttt
    33421 agcagccaca agttcttcat tgtctggatg aactgctgct gtataaacaa ccatatttac
    33481 atcatctgat atattatctt ttttctgacc aatataaata gttgcccctt ggtccctaag
    33541 tttgtctaat aggtatgatt cgtttgaatc cgaacctgaa acttgataac ctttattgat
    33601 acatatttca gctaatgcac tcatgctgat accgccaatt cctataaagt gtatattcat
    33661 aataaaacca cctttctttt taattaagtc ctacaatatg aactatttat gtggtataat
    33721 aatataatgt tcaatatttt ataattaata tgcatataat attatatgta ttttattttt
    33781 gaatataata aaacataagt aatattatac cacaataaga attgttatgc attagttagt
    33841 ttaacgttat ataaaggtac ttgtaggagg aaataataat gaaatttaaa agaacagaga
    33901 gaattggagc aatagttaaa atactgtctg ataatccaaa taaaatatat acattaagtt
    33961 attttacaaa tcaatttaat gcagcaaaat caacaataag tgaagattta ttagttgtaa
    34021 aaaatgtatt tgagaagcta catttaggta aagttataac tatctcagga gcagcaggag
    34081 gagtaaaata tatacctaaa acttctatag cagaaaatgc agagttctta atggaattat
    34141 gtgagagaat atgtgacaag tctagaatac tatctggtgg ttttttatac ttgattgacc
    34201 taatttatga ccctacaata gctgctaaga taggaaagat attcgcatca aatattgagt
    34261 atgtagatgc agattatgtt gtgacaatgg aaacaaaagg tatacctatg gcacttatga
    34321 ctgcaaaagc tatgaattta cctctagtta taataagaaa agatataaag gtatcagaag
    34381 gtcctacact tagcatgaca tatgtgagtg gaaacagctc taaagttgaa agtatgagtt
    34441 tgccaagaaa agctcttaaa ccagatagta aagttataat tatagatgat ttcatgaggg
    34501 gtggaggtac tataaaagga atggtagacc ttatgaatga atttggtgca gaggttatag
    34561 gtactggagt attcatatct acaacgaatc cttcagaaaa aatggtaaaa gattatattt
    34621 cattgattca attggatgtt aatggagata aaatagtagt agaaccaaac ttaaagactt
    34681 ttaaagatga atatagaaat gaagatttag acgaagaaga agattcagaa tttgaatttg
    34741 agatagatga agattaataa aaaaaatatt tagaaaaata tgtaaaaata aaaagaggat
    34801 atccctagtt gttcatagaa ttatttaaaa aacagtttat attaatttat tccagcgata
    34861 ataattttaa tataaattaa tttctgtcaa aatcaaaggg ggatattttg gtatgaagat
    34921 aactgacgta agagtaagaa aattaacaga agaaggtaag atgaaatgta tagtttcaat
    34981 aactttcgat aatttgtttg tagtgcatga tataaaagtt atagaaggcc ataatgggct
    35041 atttatagca atgcctagca gaaaagtagg cgaaggaaat ttcagagata tagctcatcc
    35101 aataaatgca gaaatgagac aagttttaga agatgcagtt ttacaagcat atcatgaagc
    35161 attagttcaa tgggaagtag ctgctgaata ataaatatat aaaatggaga gagcccgaac
    35221 atttaggctc tttttaaatg tagaatttct attaaaatat gtacttttta gtaaaaaagt
    35281 gctatattat tagtgataat gtatatgttg tatacaaatt gttagataaa tgtattataa
    35341 tgtatattga ttaagagaat aatacgaata aaattaaatt agattgaatg gagtgtatca
    35401 atgaacttta aagctataat ccttgctgct ggcaaaggaa caagaatgaa gtctaaatac
    35461 ccaaaagtta tacataaagt atgtggaaaa gaaatggtta accatattat agatgtttca
    35521 aaaaaatcag gggtaaaaga tactgtcgtg attttaggac atgaagctga ggtagttaaa
    35581 gaaaagcttg cagaagaaat tataatagcc atgcaaacag aacaacttgg gacaggacat
    35641 gctgtaaaga tggctaaaga atatattaat gatgaagata caatagttgt actttgtgga
    35701 gatactccac ttataaaaga agagacactg aagagattat ttgaatatca tatagaaaat
    35761 aaatatcatg ctacagttct tacaacaaga gttggaaatc caacaggtta tggtagaata
    35821 ataagagata agaagggtga ccttcttaaa atagtggaac aaaaagatgc aaattcagaa
    35881 gaaaagatga taagtgagat aaactctgga atttattgtt ttaatggaaa gagtcttaga
    35941 gaagcactag atttgctaaa taataacaat tcacaaggtg aatattattt aactgatact
    36001 gctaaaataa tgagagataa aggattaaaa gttggtgcat ttgcaggttc aactattgaa
    36061 gaacttatgg gcgttaattc aagagtagaa ttatcaaagg cagaagaaat tatgagacga
    36121 agaataaatg aatctcatat ggtaaatgga gtaactataa tagacactaa ctctacctat
    36181 atagaatctg atgttatgat aggcaatgat actataatat atccaggtgt tatgttacaa
    36241 gggaaaacaa ggataggttc agattgtata attggaatga actcatctat aacaaattca
    36301 gaaataggtg atggtacaga aataaaaaat tcaactataa ttgatagtaa ggttggagaa
    36361 aattctaatg ttgggccata tgcatactta agaccaaaga gtgacttagg aaataatgta
    36421 aaaataggcg attttgtaga agtaaaaaat gctattatag aagatggatc aaaagcatct
    36481 cacctttctt atataggaga tgcacatgtc ggtaaaaatg taaatattgg atgtggagta
    36541 gtatttgtaa actatgatgg taaaaataaa tttaaatcag tagtaaaaga caatgcgttt
    36601 ataggttcaa attctaattt agttgcacca gtagttgtag aagaaaaagg atatatagca
    36661 acaggttcaa caataacaca tgatgtccca gatggtgctt tggcaatagc aagagaaaga
    36721 caagttatca aagaaggatg ggtagaaaaa aagaatcaaa aagacgacca aagtaagtaa
    36781 ttatgtagtt tattattttg gctaaaaaat ttagctcgaa attattataa attatattca
    36841 ttagtatttt aaaattttca ggagggttac ataaatgaat actagcggaa gcgagattaa
    36901 aattattgca ggtaattcat cgaaagagtt agcacaaaaa atagctgact atataggtgt
    36961 atcagtacta gattgtgaag taggtacatt tagtgatggt gaaatatgtg taaacatgaa
    37021 tgagacagta agaggttgtg atgtgtttgt agtgcaatct actaacagcc cagtaaatga
    37081 taacttaatg gagttattaa tcttaattga tgcgttaaag agagcatcag caggtagaat
    37141 aacagcagtc attccttact atggatatgc aagacaagac agaaaggcta aggcaagaga
    37201 tccaatcact gctaaattag ttgcaaattt aataactgct gctggagcag acagagtatt
    37261 aacaatggat ttacatgcag ctcaaataca aggatatttt gatataccat tagaccactt
    37321 actaggtgga acaatattag caaattattt taatgaaaag aaaatagaag atttggtagt
    37381 agtatctcct gacttaggaa gtgttactag atcaagaaaa tttgctaata ctttaaatgg
    37441 agaagttcca atagctataa tagataaaag aagaccgaaa gctaatgtat gcgaagttat
    37501 gaacttaata ggtgatgtta aaggtaaaaa tgttatacta ttagacgata tgatagatac
    37561 agcaggaaca atagttaatg ctgcaaatgc acttaaagag tttggagcaa aagatgttta
    37621 tgcttgttgt actcatggtg tgttatcagg tccagctatt gaaagaattg ctaattcaga
    37681 aataagtgag ttaatagttc ttgatactat acaacttcct gaagagaaaa gaatagataa
    37741 gataaagata aaaacagtag ctcctttatt tggagatgct ataagaatga tattctctaa
    37801 tgaatcagta agtaaattat tctaatttat tgtatttatg agagttgttt aaaaggatat
    37861 aaaccttttg agcaactctt ttttgatttt atacttatgg aatgttattt ttttcttgtt
    37921 agaaaggtat atattattgt aaaaagagat tatagtttaa aattatgtat atttaagtaa
    37981 aataaactta ataaagggca tattattcaa aatattatga atataaaaaa tattacaaaa
    38041 aagtattgaa aaaattcaga ttatgtagta ttatgtaaat ataaataaag aaagaggtgt
    38101 ttccaatggg aaggttagaa gatgaacccc taataattaa atgattcagt cacaatctaa
    38161 agtgaatata aaaatctatt gaaagtttat ttataagaat tgaaaatagt atctattcta
    38221 tctttagtgt ttgtgagagg gttctggatt atataagatt actgtaaaga gtaattttgc
    38281 acaataagca tagctgtaaa tgaagagttt cgtttatata atgattgtgg ctgaatcata
    38341 actataatag atagaatgtt tataatagat gattttataa aatactatat tgtaatagct
    38401 tttagctttc aaataaatat tagcaaagga tggaaatgac aatgataaaa ctaaatgata
    38461 atttataaat atctccttac gatatataat aattgtaggg agatgtttct tttttttgta
    38521 gagtataa
//
LOCUS       DAELSY010000022        36447 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.022, whole genome shotgun sequence.
ACCESSION   DAELSY010000022 DAELSY010000000
VERSION     DAELSY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36447
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.022"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(27..872)
                     /locus_tag="KPG09_001003"
     CDS             complement(27..872)
                     /locus_tag="KPG09_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0965627.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCTYQDTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSGKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            1089..2255
                     /locus_tag="KPG09_001004"
     CDS             1089..2255
                     /locus_tag="KPG09_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBG0965628.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNIVLDTHQYLMLAESDGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            2277..3923
                     /locus_tag="KPG09_001005"
     CDS             2277..3923
                     /locus_tag="KPG09_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBG0965629.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYIHSSGFDASILHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWRPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNVLGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIADESKDVD"
     gene            4020..5393
                     /locus_tag="KPG09_001006"
     CDS             4020..5393
                     /locus_tag="KPG09_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0965630.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNYIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMFFYKLDKEYSTI
                     LNELNTK"
     gene            5838..6791
                     /locus_tag="KPG09_001007"
     CDS             5838..6791
                     /locus_tag="KPG09_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBG0965631.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            6829..7791
                     /locus_tag="KPG09_001008"
     CDS             6829..7791
                     /locus_tag="KPG09_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBG0965632.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            7816..8802
                     /locus_tag="KPG09_001009"
     CDS             7816..8802
                     /locus_tag="KPG09_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBG0965633.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            8815..9861
                     /locus_tag="KPG09_001010"
     CDS             8815..9861
                     /locus_tag="KPG09_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBG0965634.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYSGKIRKCDVEKLTAKETIVSNNTSKYSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            10028..11758
                     /gene="lpdA"
                     /locus_tag="KPG09_001011"
     CDS             10028..11758
                     /gene="lpdA"
                     /locus_tag="KPG09_001011"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG0965635.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEVPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            12176..15124
                     /locus_tag="KPG09_001012"
     CDS             12176..15124
                     /locus_tag="KPG09_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0965636.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            15102..15566
                     /locus_tag="KPG09_001013"
     CDS             15102..15566
                     /locus_tag="KPG09_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0965637.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            15679..15981
                     /locus_tag="KPG09_001014"
     CDS             15679..15981
                     /locus_tag="KPG09_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0965638.1"
                     /translation="MGVTNVTKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            16043..17407
                     /locus_tag="KPG09_001015"
     CDS             16043..17407
                     /locus_tag="KPG09_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG0965639.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            17442..17915
                     /locus_tag="KPG09_001016"
     CDS             17442..17915
                     /locus_tag="KPG09_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965640.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYINFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGDLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLRFRNKKFENKNI"
     gene            18087..18737
                     /locus_tag="KPG09_001017"
     CDS             18087..18737
                     /locus_tag="KPG09_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG0965641.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTFKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            18911..19843
                     /locus_tag="KPG09_001018"
     CDS             18911..19843
                     /locus_tag="KPG09_001018"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG0965642.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEDSIFIG
                     KNQNPYQEVHHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYRKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            20117..20830
                     /gene="ispD"
                     /locus_tag="KPG09_001019"
     CDS             20117..20830
                     /gene="ispD"
                     /locus_tag="KPG09_001019"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBG0965643.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            20827..21312
                     /locus_tag="KPG09_001020"
     CDS             20827..21312
                     /locus_tag="KPG09_001020"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBG0965644.1"
                     /translation="MRIGLGYDVHKLTENRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    21407..21615
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            21679..23394
                     /locus_tag="KPG09_001021"
     CDS             21679..23394
                     /locus_tag="KPG09_001021"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG0965645.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDQCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            23516..24961
                     /locus_tag="KPG09_001022"
     CDS             23516..24961
                     /locus_tag="KPG09_001022"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG0965646.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKTKGVAVEVDDRDNYT
                     TGWKFNEWEMKGAPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            25401..26882
                     /locus_tag="KPG09_001023"
     CDS             25401..26882
                     /locus_tag="KPG09_001023"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBG0965647.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLVEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEITEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            27103..27258
                     /locus_tag="KPG09_001024"
     CDS             27103..27258
                     /locus_tag="KPG09_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965648.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            27403..28800
                     /locus_tag="KPG09_001025"
     CDS             27403..28800
                     /locus_tag="KPG09_001025"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBG0965649.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKIFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            28785..29198
                     /locus_tag="KPG09_001026"
     CDS             28785..29198
                     /locus_tag="KPG09_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBG0965650.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            29305..29400
                     /locus_tag="KPG09_001027"
                     /pseudo
     CDS             29305..29400
                     /locus_tag="KPG09_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase (FAD)"
     gene            29706..30536
                     /gene="thyA"
                     /locus_tag="KPG09_001028"
     CDS             29706..30536
                     /gene="thyA"
                     /locus_tag="KPG09_001028"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBG0965651.1"
                     /translation="MSLADKIFIDMCNNILENGVSSDGEVVRAKWDDGQPAHTIKKFC
                     VVNRYDLSKEFPILTLRPTNLKAAIDELLWIWQKKSNNINDLNSRIWDSWADETGSIG
                     KAYGYQIGQKHKYKEGEFDQIDRVIYDLKYNPYSRRIITNIYNHNDLNEMNLYPCAYS
                     MTFNVTGNRLNAILNQRSNDILVANNWNVTQYAILVHMLAQISDLEVGELVHVIADAH
                     IYDRHIPLVKELINRESYDAPKLIINPNIKNFYDFRVEDFTLENYKSGPQIKKIPVAI
                     "
     gene            30549..31043
                     /locus_tag="KPG09_001029"
     CDS             30549..31043
                     /locus_tag="KPG09_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBG0965652.1"
                     /translation="MKVIVAVDLNWGIGYGGKLLQSIPEDMKFFKEKTVGNVVVMGRE
                     TFKSLPGKKALKDRVNIVLTRSKSFNDDRLIVCNSIDETLKELQKYENDKIFIIGGET
                     VYKQFLPYCNELYVTKIQKTYEADRFFPNIDIMSNWDLIKESEVKEYNNIRYVFSTYI
                     NNSL"
     gene            31070..32446
                     /locus_tag="KPG09_001030"
     CDS             31070..32446
                     /locus_tag="KPG09_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBG0965653.1"
                     /translation="MKKDIFVNRVSNALENDRGAVFVGSGISFSSTEVDWFKLLEPLV
                     NELDITLDNKSDDLPMIAQYIVNNYSGNRGPLVNEISKTFNRKFKLNKYHKYLATTKL
                     STIWTTNYDMLLEDAFYDFDTSVKVSDDSITRNRLEYDVEIIKMHGCISRSDWNEIVI
                     TQEDYDDFSINKPAISQRLSEDLINKSFLFIGYSYRDPNIRNIMINARRLSKKHTQQH
                     YIILKKLKDSDSNITQDDVVRQELWCNDLKRLGISTLFIDEYSELEELLREISQKSRG
                     KTVYVTGSHKESNMNARKLGTKLAKHKDITLLSGQSSGIGSEVVAAFTEECIKSKEDI
                     INRMKIFPNPYSADEKYSDDITLIPDLKKCRTKMLNSTQVVVVFSGGIGTEAEIDVAI
                     KQNCNIIPVITCKEDRENPAIKKILNYSDNMKNIEIKDATYYNKIVDEDGVVTMEEIY
                     ICISKIMN"
     gene            32422..33522
                     /locus_tag="KPG09_001031"
     CDS             32422..33522
                     /locus_tag="KPG09_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBG0965654.1"
                     /translation="MHIKNNELKHDIIKLKKRNGFIEVGKDKKTLVNCLIGANELEDY
                     KYEIKKIDCILNISEKPDIVSDLSTKRRKISESIWYKIVKDTPFVSATLPIYLVSKNN
                     NKIDSNELLDIIIEQMENGVGMITIHPTANKQIFEDSKNRMVPVTSRGGGMVIQDLIS
                     KNFSEDNVYLKILPEIIKYAQKHNVCISLGATFRSGNIFDSNDRAQITEIKSQIELAK
                     RIYKSNVGVIIESPGHSRPSDIIKISSILKKEGFPIMPLGPIPTDIAIGMDHIAAAIG
                     ATIMGIEGCANIISTVTREEHTGGRPSIESTVESIRTAKIAAHIIDIHQMNDINLDMS
                     VSKSRAKSNTCIFGKDTRYCDRCRELCPLCIR"
     gene            33565..33990
                     /locus_tag="KPG09_001032"
     CDS             33565..33990
                     /locus_tag="KPG09_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBG0965655.1"
                     /translation="MHRIASFSQQSQRYVKLEQFEYIIPPEIEKIEKAKELFINSMKK
                     YQEDYDKLVEILFENHYNKLIKGGKNEKTSKRQVEKKAIEDARYVFPNACETKMVFTI
                     NARSLFNFFEHRCYENGSCLEGTMTCGDIVQVRKKFKNL"
     gene            34003..34740
                     /gene="rlmB"
                     /locus_tag="KPG09_001033"
     CDS             34003..34740
                     /gene="rlmB"
                     /locus_tag="KPG09_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBG0965656.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            34743..35270
                     /locus_tag="KPG09_001034"
     CDS             34743..35270
                     /locus_tag="KPG09_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBG0965657.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            35335..35982
                     /gene="sigH"
                     /locus_tag="KPG09_001035"
     CDS             35335..35982
                     /gene="sigH"
                     /locus_tag="KPG09_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBG0965658.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            36056..>36447
                     /locus_tag="KPG09_001036"
     CDS             36056..>36447
                     /locus_tag="KPG09_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBG0965659.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVP"
BASE COUNT        14854 a         3813 c         6843 g        10937 t
ORIGIN      
        1 agataaataa catataacct catattttag gtagattttt ttcttatatg tttaggagtg
       61 catttaaatt tttccttaaa aattctgtaa aataattttg tattagtaaa tccatgagct
      121 tctaatatac tgcataaggt atcatttgtg ttaattaagt ctttataaat atgagaaata
      181 cgcacatcat ttaaatactc taagtaagtt tgtcccatat actgtttaaa ttttctacaa
      241 aaatattctg gctgtaagtg tgctacctta gcgattttgt taatagaaat aggcattttg
      301 taattaatct ttgtatattc taaaactggt tcaaattttg acaaatcaag tactgttttt
      361 ttcatatttt tattacctac agatatttta aagtttgtat ataattcaaa taaaagttca
      421 aataataagc ttgtaaagag cagattagct gcaggaggag agctaagttt aatttctctc
      481 atgttttcta atataagttt aactttacct gaattttttt tatagttatt gtcattggca
      541 ttaaccttaa agtcaaaata gcaattttta aaatcaggga tatatttgtt tagaaaagac
      601 aatggtactt gcaataatat agaggtatct tgataagtac atctagttga gtgaataatt
      661 ccagaattaa tgaacataca ctcattagct tttaaattat aagtgtaacc ctccatgtta
      721 atttgtaaat caccagaagt tatataaatt atttctaatg agttatgcca atgatttgct
      781 acataactac cactatcatc aaatagtttg aaattaacat ctaaattatc aataggttca
      841 attatttcat gtgaaaaatt atttctgttc ataataatat cctccttata attttaaatt
      901 ataaatagta aatttataat gttcaatatt aaaaagataa tatagactaa taaattagat
      961 aataaacata cttaagaaaa cggataaaat caaatataat aactgtatat taaaataact
     1021 taaaaaaatt attttattat aagtgcaaaa tatacctatt taaataagat aagaggggag
     1081 gattttatgt gtatataaaa ggtgtaaatt taggaggttg gttagtttta gaaaaatgga
     1141 tgacctcaag tttatttgaa ggcacagaag ctgaagatga atactattta ccaagacagc
     1201 tttcaagaga ggcatatgaa tctagaatta agactcatcg tagtgaatac atcacagaga
     1261 gagattttgc aacgattaaa tctatgggat ttaattcagt tcgtatacca gtaccatact
     1321 ttatttttgg agattgtgaa ccattcatag gatgtgttaa agaacttgat aaagcatttg
     1381 cttgggcaga taagtatggg cttagtatat tgatagactt acatacagtt ccaggaagcc
     1441 aaaatggatt tgataatgga ggaatatcag gtatatgtag ctggtcacaa aatccagaat
     1501 atgtagcgtt tacattaaat gtattagaaa gacttgcaaa acgttatggg atgagacatg
     1561 aattgtatgg aatccaaatt ttaaatgagc ctataacaga aagaatgtgg aacataatga
     1621 atgttccaaa tcggtttaag gctgtagata aggagatggc aagggggagt aaacctaatt
     1681 cattagagtt tttacgtgat ttttatataa aagcatataa ggtaatgcgt ccttatatga
     1741 gagaggaaaa cgttatagtg tttcatgatg catttgaatt aaaagcatgg aaagacttta
     1801 tgagagagga agaatttaaa aatattgttc tagatacaca ccaatatttg atgttagctg
     1861 aatcagatgg atgtgaacaa agtattgata gctatctcaa atatattaga gaaaattatg
     1921 ctaaagatat tttacaaatg caaaaatatt ttcctgttat atgtggggag tggagcttat
     1981 ttaattcata tgcttgtggt atagatacaa atggaggtca atcacctcta aatgggattg
     2041 aatcaaatat agataaatta tcaaaagacg ataaaagaga attatatagg aagatagcaa
     2101 aagcacagct tgatgcatgg agaaatggaa gtggtcacta ctattggaat tataagttat
     2161 tactagatac agttaatgaa gaaggttgga taggatggga tagttgggac ctaggcaaat
     2221 gtgtagctca agaatggtat cctatagaat attaaactaa aattggagga aaagatatga
     2281 ttaaaaaccc tatacttcca ggttttaatc cagatccttg tatttgtaga aagggagatg
     2341 attattatct tgtagtttca tcttttgaat ggtttccagg tatacctgtt tatcactcta
     2401 aagacttaaa aaattgggag ttatatactc atattttgac agatgaaaca aaaatagatt
     2461 tgaagaagct tccttcatct aaaggaattt gggcaccttg tttgacttat tgtgaagaag
     2521 aagatttatt ttacatagtt tatggaatta tgaactctat gaatgcaaga tattttgatg
     2581 ttgataatta tttaattaca tcaaaagata taaagggaga gtggagtgaa ccagtatata
     2641 tacactcatc aggttttgat gcgtctattc ttcatgatga tgatggtaaa aagtggattg
     2701 catcattaga ctgggagact agagaaggat atgaaaaacc tggtgttata tgtttagtgg
     2761 aatactgtac aaaaaagaaa gaaatagtag gatatccaaa gagaatatgg tcagggggaa
     2821 ctgatagagg ttgtattgaa gcaccacata taacaaagcg tggagattac tattatataa
     2881 tgtgtgcaga aggtggaact ggatatggtc atagtgtaac tatgggaagg gctaaaaata
     2941 tatggagacc ttatgaaaaa gattcaatga atccaattgt aacttcaatt ccaggcgatt
     3001 tttatgaaag acatgaccct gaccatctaa agcctaaata ctataatcca gaatcaaaac
     3061 ttcaaaaatc tggtcatgga agctatatag aaacaacatc aggtgaagta tatttagttc
     3121 atctaacatc tagaccattt gtaccagagt taaggtgtac tttaggtaga gaaacagcta
     3181 ttcaaaaaat gaaatggaca aaggataatt ggcttcgaat ggaagatgaa agtaatttgg
     3241 caaaagaata cgtatcagaa agtaaactag aagaacattt agtttcatct ataccaagtt
     3301 ttgatgactt tgattcaaat gtattaggtt tacagtacta tgctcctagg atttcaccat
     3361 tatcatttgc agatgttaag tctagaccag gatatgtaag gattagaggt caagaatcaa
     3421 gaacatcatt aaataaagta agcatattgg caagaaaatt aacttcagta tatgcaagaa
     3481 taaccactaa gatggagttt tacccagagg tacatcagca tagtgcgggt ttgataatgt
     3541 attatgataa tatgaattat ataaatttaa gaaagtatta tagtgaaaca ttgggtcaaa
     3601 gtgcactatc aataatacat cttgaaaatg gagagaagac ggaattttta aatactagga
     3661 taccaataaa agatatacca atatatcttc gtctgtatat acaaggtcgt aagtcatatt
     3721 ttgaatggag ttatgatgaa aaaaactacc aaagaatagg aaaggtattt gatacaacta
     3781 agttttcaga tgagtactgt aaatatggag aatttacagg tacgtttata ggattaactt
     3841 gtgcagatcg tgtaaagcat aaacactatg ctgattttga tttttttgaa tatatagcag
     3901 atgaatcaaa agatgtcgat taataaaatc attactaaaa ttttcagagc aaaggtaaat
     3961 cttatgctct gaaataagaa acattagaag gcaaatctta caaaaaggag tatggtatta
     4021 tgaatacaga agttaatgtt aaagcaacta cgattagata tgaaaagatt tcattttttg
     4081 aaagaatttc ttatggttgt ggggatttgg ggtgcaatat catatactca gctatgtcag
     4141 catttttgct gttttattat actaattatg cagatgttag tgcagcagct gtaggtagta
     4201 ttatgcttgt atctaggata ctggatgggt ttagtgactt aacaatggga attatagtgg
     4261 atagaactaa atccaaatac ggtaaggcta ggccttggat tttgagaatg gcgattcctt
     4321 ttgctatagc agcagtatta ttattttctg ttccatcaaa tcttggtatc acttctaaat
     4381 taatttatat ttttattaca tataacttag tttctactgt tatttataca gcaatcaatg
     4441 taccctatgc aactctaaat tcacttataa ctcaaaacca gtatgaaaga ggagtattaa
     4501 gtatatttag aatgatatta gccacttgtg gtaccttact aataaatgga cttacactac
     4561 ctctagttga atactttgga aataatttat cagcatggac taaaacattc tttgtatttg
     4621 gaatagcatc tataatggta ttttttataa catttactgg aaccaaggaa agagtaaaag
     4681 cagttaagca agataggaat gaagtaatac catttaaaat aggtattaaa tctttattta
     4741 gaaataagta ctggatacaa ataacattat gtttagtttg catatttatt gtatttgcga
     4801 taaatggtgg ttcatccgta tattatgcaa agtttatttt gggagatgaa aaattatttg
     4861 gtcctataaa tatggtttca aatatatccc aaattatagc tatgtttatg gttgctcctt
     4921 ttataaaaaa atttggaaaa agaaatgtat taatagtagg ttcaataata ttaatatcgt
     4981 caaatgttat gtttataata gttggacaaa actatatagg agtaatattt gcaagtgcta
     5041 taaagggaat aggaagtgct ggaatagcat caacaatgtt tgctattgtt tcagatacta
     5101 ttgaatatgg agaatggaag actggatata gaactgaggg gcttataaat agtgcatcta
     5161 gttttgggtt taaagtggga aatggattag gttcagcaat attaggtgca gtattatcaa
     5221 ttggtggtta tgtaggaaca tcagctactc aaagtgattt agctatagta tcaataaaag
     5281 tatgttttat atacctacct atatttataa caatattaca aattatcata atgttttttt
     5341 ataagcttga taaagaatat agtactatac ttaatgaatt aaatactaaa taatgaaaaa
     5401 gtttcttata aagtcttaag tttaattaag gtctattgta aatgttccct ataaaaacta
     5461 ttatatattt atgattattg aagaattttt agagaagcga ataataaaaa tataagaaga
     5521 ctttaaatga tgaaatggtt ggtcttttag aaatgattta ataagttatt tttatttaaa
     5581 gcattataag tggacaatta atttttaatt tatggagatg atatttaaca attagaagta
     5641 atatataata tgcgctaaga agcaattttt caactattta tgaaggtggt ggtacaagaa
     5701 aagtatcacc ttttttttgt tatacaaaaa aacagttgac tttttatatg aaccccttct
     5761 ataatgttac tgaaattaag taaaatgatt gaacaaatgt aaagataatt gaaaagcttg
     5821 attttggagg aaatcaaatg aaaaaaattg gagacttaag attaatgatg aagtgttgtt
     5881 cactttatta cgaagataat ttgaatcaac aggaaatagc aaatcaattg ggaatatcaa
     5941 gaccaacaat atcaagaata ttaaaagaag cttttgaaca aggaatagta aaaatacaga
     6001 tagttgatgt tttgaaaaat gattaccaaa aaatagagag aagtttggag agaaaatata
     6061 aattaaaaga agtaatagta gtagatgata aacaagatgc actaacacaa aaacaagaac
     6121 tagcaagagc tgtatctgaa tatttaacta gagtagtaaa ggaaaatgat ataataggtg
     6181 tttcaatagg aacaacacta aaagaaatac caagatatgt ggaaaaaagt aactgtaaaa
     6241 atgttacatt tatacctcta ttaggaggga ttggagacaa tgaaatagat attcatgcaa
     6301 atcaaatagc agtaagtcta gcgagggctt ttggaggtga ttttaaatta ttacatgctc
     6361 cagctgttat gtctgacttg tctacaaaag agaaactttg caaagacgaa aaaattaaag
     6421 aagtactaga tttaatagat aaaacaacta tagcaattgt gggtatagga aatcctatga
     6481 gtttaaactc aacaataatg gcttctggat acatgaatga agcagatata gaagatttaa
     6541 aaaaatacaa ctcaataggt gcaatttgtt tacaagcctt tgataaagaa ggaaaaactt
     6601 ctatacttga atttaatcaa agagtattag gagtgaaact ggaagattta aaaaagataa
     6661 aaagaacaat aggtgtcgct tcaggagatg aaaaaataga ggcaataaag gcatcattaa
     6721 aagcgaaatt tataaatagt ttagcaatta atcacagttt agcacttaaa ttattagaag
     6781 attgtgacta atagaaagat agataaaagt taacaggagg gaatgtatat gtcaatatca
     6841 aaagaaacat tgcttgaaat gtataagaga atgaatcagg ctagaaaatt tgaagagaaa
     6901 gttagttggt tttttgcaag aggtatggtt catggaacaa ctcacttatc tgtaggtcaa
     6961 gaagcgtcaa gtgtggcggc tgtaatggct ttagaaaaag gagatttagt atcattaact
     7021 catagaggac atagtcaatt tataggaatg ggaatagacc taaataaaat gatggctgaa
     7081 cttatgggta aagaaactgg attttgtaag ggaaaaggag gctcaatgca tatagcagat
     7141 attgagtcag gtaacttagg agcaaatgga gttgtaggtg gaggtcttac aatagctcca
     7201 ggagctgcac ttacacaaca gtataaaaaa acaggcaaaa tagttttatg tagttttggt
     7261 gatggagcat caaatgaagg tacttttcat gaaggaataa atttatcttc aatatggaaa
     7321 ttgccaataa tattttactg tgagaataat ttatacggta tgtcaacaag cataaaacgt
     7381 catatgaata tagaaagtat tgccactaga gcagcttctt atggaataga aggaatatca
     7441 atagatggat ataacccaat agaagtatac gaaacagtac aaaaagctgc tgagaagtgt
     7501 agaagaggag aaggacctgt attaattgaa agtagaactt atagatggtt aggtcattca
     7561 aaatcagatg caaatgtata tagaacaaaa gaagaaatag aatcatggaa agcaaaagac
     7621 cctattgaat ttttaaaaaa ttatcttata gaaaataatc tatctaatga agatgaatta
     7681 gataagattc aagaatttgc aaagcaatca atagaagatg cagtagagtt tgctcaaaat
     7741 agcccaaatc caaaaataga gtctttatta gaggatgtat atgcagacta agaatggtaa
     7801 aggaggaaaa taaaaatgag tacaagggaa ttaacatatg cacaagcaat aaaggaagca
     7861 atgtcagaag aaatgagaag agatgaaaat gtaatattta tgggagaaga tataggaata
     7921 tatggagggg catttggtgt atcagttggt atgatagatg aatttggacc agaaagagta
     7981 agggatactc ctatatcaga agcagctata gcaggagcgg cagcaggtgc agcagcaact
     8041 ggtttaagac ctattatgga agtaatgttt atggactttg taacaatttc tatggatgca
     8101 attgtaaatc aagcggcaaa aatgagatat atgtttggag gaaaagcaca agttccaatg
     8161 gttgttagat gtccaggtgg ttctggaact ggttcagcag agcaacattc acaaagttta
     8221 gaagcttggt tttgtcatgt accaggagta aaagtagttg caccttctac accagcagat
     8281 gctaagggat tgttgaaagc agctataaga gataataatc cagtcatatt tgttgagaat
     8341 aaattgttat acagaaaaaa aggttttgtg ccagaagatg attatgtaat tgaaattgga
     8401 aaggcagata ttaaaagaga gggtactgat gttacagtaa ttacttatgg aagaatgtta
     8461 caaagtgttg aagaagcagc agagactcta tcaaaagaaa atataaatgt tgaaattata
     8521 gatttaagaa ctttatatcc attagataaa gagactatag taaagagtgt atgtaaaact
     8581 gggagagttt taatttgcca tgaagcagct aagactggtg ggctaggtgg agaaatatca
     8641 gccctgataa cagaaagtga atcatttgat tatttagatg ctccagtaaa aagaatttgt
     8701 ggaaaagatg tgccaatacc atataatcca gagttagaaa aagcagttgt cccaagagtt
     8761 gatgaaatag aagaagcaat aaaatcttta attgttagat aaggagggat agatatggtg
     8821 gcaaataaaa taaaagccac accagcagct agaagtcagg caagaaaaga caatataaaa
     8881 ttagataggc tgattggaag tggagaaaat ggaaggattc atttagtaga tgtgttaaat
     8941 tatttaaaag ataataaagc aaatactact cctttagcta gaagaatagc agaagattta
     9001 aatatagatt tagaaactat tgtaggtact ggatatagtg gaaagataag aaagtgtgat
     9061 gtggaaaaat taactgcaaa agaaactatt gtatcaaata atacatctaa atatagtgag
     9121 aaaaaagagc tgaaaattga aaatgaaaat tcaagcatgt ttaacacagt agaaggaata
     9181 tttgaaaagc caaatccaat gagagctact gtagcaaaac gaatgtcaga aagttatttt
     9241 agtgctcctg tatttacatt caatatagaa gtagatgcta cagaattgaa ggtacttaga
     9301 gcaaagttaa tcgacacagt aaaagaatca actggagtaa aattaactat gacagattta
     9361 atagtaatgg cagtatctaa aatacttcca aatcatcaag ccttaaattc agcatggaca
     9421 gatgagggta tatttagata taaagatgta aatatagcta ttgcagttgg tttagatgaa
     9481 ggcttatatg ttccggtagt aaaaaatgca aacaaaaagt ctttaaaaga aatagcaaaa
     9541 gaaagcaaag aacttgcgga aaaagtaaag acagggaaac ttatgccagc agaccaagaa
     9601 ggtaatacat ttacaataag taatgttgga atgtatggta taactacatt tacacctata
     9661 ataaatatgc cttcaagtgc aatcttaggg gtaggtgcta cacaagataa gtttgttcca
     9721 gtaaatggag aggctaaaat aaaacctata atgaacttat cattaacatc agaccataga
     9781 gtaatagatg gaacagtagc tgcaaaattc ttaaaagatt taaaagaatt gttagaaaat
     9841 cctttatcaa tgcttgtata ggttttgata tttcgttact gatataaata gtttgaatag
     9901 attatattat tataattgtt tcagtaaatt taaagctttt atatattaag aattattttt
     9961 aacagtaact atatgtgaat atgatttgct taattaaaat atatgaaaat aaataggagg
    10021 agagcctatg tcagtagaag taataatgcc aaaagcagga gtagccatgg aagaaggtac
    10081 tatagtatct tggctaaagc aagaaggcga agaagtaaag attggagaac ctatactaga
    10141 aataacaaca gataaagtta atatggagat agagtcagaa ggagaaggta cacttgctgt
    10201 aatcatacat aaagaggaag gtgaagtatt accagtattt acagtaatag gtgtaatagc
    10261 agaaaaaggg gaaaatcaag aggaagtaaa agctaaatat ttatctggaa atgtttcaaa
    10321 agaagatata gttgaagaga atcaaaatat agaagttaaa gaagaaaaaa taaacaaaaa
    10381 agagtgtaat catgattatg atgtagtggt aataggagga ggacctgggg gatatttatc
    10441 agcattaaaa gcagctcttt taggtggaag agttgcttta gttgaagaaa atatactagg
    10501 aggtacttgc ttaaatagag gttgtatccc tacaaagact tatataaaaa cagcagaaat
    10561 attagaagaa attgaccaat tatcaaagag aggtgtaaaa gtaactgtag ataaagaaca
    10621 ggacattaag aaggccataa aatataaaaa tagagtagtt aaaaaactta cagctggtgt
    10681 tggcggatta ttaaaaagta gagatgtaga tgtgtttaat cttaaagcta gtgttaaaga
    10741 agagcataaa gtaattttat cagatggaaa agttttagat acagaaaata taataattgc
    10801 tacaggttcc aaagtaagga tattacctat aaaaggaata gaatcaaatt taataataac
    10861 tagtacagaa gcattagatt tggaaacagt gccagaagaa ttagtaataa ttggtggtgg
    10921 agtgattggt tgtgaatttg cagagatatt caattcaaga ggttctaaag taactatagt
    10981 agaaatggaa gatagagtaa tcccaagaat ggataaagag ttaagcgaat ctttaaaata
    11041 ttctcttagt aaaaaaggta taaatgtatt aactaaaaag aaagtttctg aatttaaaga
    11101 agaaggaaat aacatattag tgtgcattga aggtgaagtg ccaataaaag cagatttatg
    11161 tctatatgct ataggaagag aagctaatct ttctggcata gaggacttag atattaaaat
    11221 agataaaggt tcaatagttg taaatagtaa aatggaaaca agtataccaa gcatatatgc
    11281 agtaggtgat gttactggag gtgtaatgtt agctcatgct gcttttaaaa tgggagaggt
    11341 agcagcttca aatgctctag gtgtgaataa agaagttgac ttaggtgcat tacctagttg
    11401 tgtttataca attccagaag tagcatcagt tggtattaca gaagaggatg ctagaaaaaa
    11461 atataatgta aaagttggta agtttaattt tgctggaaat ggaagagctt tagcttctgg
    11521 tcaagaacaa ggatatgtga aagtagtagc agatgctaaa tatggagaaa ttttaggtat
    11581 acatatgttt ggatgtggtg tagcagaact tataaatcat gcagcatcat ttaaagcatt
    11641 agagatacct actgatgaag caagtgagtt gatatttggt catccatgta cttcagaagc
    11701 tttaatggaa gctttggcag atgtaaatgg agaatgttta cacttaccta aaaaataaat
    11761 ctaaacataa attattaatt taagtctatt attaatatta atttatatca taaaatttta
    11821 aaacacttac ttagccatac cccttattat gcaaataatt tatttgtata ataaggggta
    11881 ttttttatgg aaaaatatag aatatagaat ccttgtagtt tttgttgtac aaaaaagata
    11941 gtaatataaa ataacattat cattttagaa tgatttcaat tgtaattagg aaaagtgatt
    12001 tttttgtttt taaaaaaagt attatttgta aaatataaaa aaaaatttct taatatttga
    12061 ataaaagaat agcttaattt aaatgttatt actttataaa aaaataataa gtacaaaaaa
    12121 aacaatatat ggcatataag ttgctatata aataataagg agggaacaat tataaatgag
    12181 gcaaagaata aaaaacttaa ttgaaaatga agataagaaa aatcctttaa cagatgaagc
    12241 gatttcaagt aggttaaata ttcgaagaga agatgttacc tttttgagaa atgagttaaa
    12301 aatagaagat tcaagacaaa gaagaaaagt tgttttaatt aaagctataa aagatatttt
    12361 aaaagaagaa aaaactataa ataaaaatga tataacaaga agattaaaca gcattgggtt
    12421 tacagtatca agatttacag tcatacaata cttaaaagag attcaagatt ctggtgacct
    12481 aaaacttgat aaagagagtg atgataaaaa agaagaaata atcaataata gagaaaataa
    12541 agatatagca tttgataaac tcataggtag tagtggtagc ttaagtacaa ttataaaact
    12601 agccaaagca gcaattcttt atccacctca tggtcttcat actattatta tggggccaac
    12661 tggtgttgga aagagtgagt tagcagagtg tatgtataaa tttgcagtag aatcatccag
    12721 attccctaag aatgcaccat ttatagtttt taatgcagca gattattcag agaatccaca
    12781 gcttttgcta tcacagttat ttggacacgt taaaggtgct tttacaggag ctgatgagaa
    12841 caaagaagga ttagtatcaa aggcagatgg aggaatattg tttatagatg aaatacatag
    12901 attacctcat gaaggtcaag aaatactatt tcaactgatt gataaaggta tggtcagaaa
    12961 gttaggagaa acaagactaa cacataaaat agatgtaatg attatttcag caacttctga
    13021 gccaatagat tctcatttac ttaatacatt taaaagacgt atacctgtaa caattgaaat
    13081 accagaatta gttgcaagac cattaaatga gaggtttgac attataaata atttcttctt
    13141 aatagaagct caaagaatgg gagcaaacat acatataaag tcagatgtat taaaagctct
    13201 tatgctttac gattgtatag gtaatgtagg tcaacttaga agtgatattc aagtagcttg
    13261 tgctagggga ctattaaacc aacttacaaa caaactaaaa gaagtaaata taagcatatc
    13321 agatgtacct ggatatgtaa aacaaggtct tataaaaatc agaaattgta gaggtaaaat
    13381 agaaaattat gtagatgggg atttaattat agattcaaat cttcaaggtg agatagaaaa
    13441 gaaaaatgaa gatatatata cttttccaga tgaaatatac aaacttactg aaagaagaca
    13501 tattgattta ttggaacaag gtttagattt tgatgttata aatagaatca ttggaggaga
    13561 attagaaagt tgcattcaaa aatacataaa acaagttaaa aaagtttcta ttagtaacaa
    13621 tcttccagat ataagtccaa ttgttggaaa atatatcgta gagctaactt atgaagtaat
    13681 caaaatagca tcacaatacc taagttcact agaaccgaat ctacatttat gtttagcagt
    13741 tcacctaaaa gcagcttttg agagattaaa agaaggaaaa gtaatatcaa atgctcattt
    13801 gaaagaaata ataggaaatt atccactaga atattctgta gcagaaaagg tagctcaata
    13861 tttaaaggaa agatataata taaacttacc aaaagaagaa ataggtataa tagcaatata
    13921 ttttaaaatg agtagtagaa gagatatgtc tgaacaaaaa aagataggtg tacttcttat
    13981 ggctcacgga agagtagcat cttctgtatg tgaggttaca aataaactat taggaattaa
    14041 tcacgcaagg tatttagata tgccatttga gaagaagccg gaagaaatga ttgaagaagc
    14101 tactacaata ataaaagata ttgatgaagg tagaggtgtg attatattag tagatatggg
    14161 ttcactttca tattttggag atattatttc tggaaagact ggaattaata taaggacaat
    14221 atctagattt gatacattgc tttctataga ggttatacga agagcagttt taccagattt
    14281 aacaatagac gagatagttg atgaagttaa aggtatagag ccacaagtaa aagaaaaggt
    14341 tagtaaatgt aacaatacaa aagtagattt tagaaaacct ataattttaa cattatgtat
    14401 tactggatta gggggagctt gtaagataaa aaaaatccta gaggaaaata ttaaaagtat
    14461 aactgaaaag gttgatatag aacctatagg tatgattgaa tatccaagtc ttgatttaga
    14521 gataaaacaa ataagcaatg ataaaaatgt aatcgccata gttggaacaa ttgaccctca
    14581 gatagcaaat atcccttttt ttagtctagt agacataaat gataaagcaa agttaaatca
    14641 aataaataac ctaattgata caaatcaaat tggtgaaaca aataaaaaat atgaattatc
    14701 agatttaata cataaagaag atatatttgt ggatttacag gttaaatcaa aagaagaagt
    14761 tctaaaatat atgagtaaaa aacttagaga gaaaggttat gtgacaaaaa atttctataa
    14821 aaaagtagtg gaaagagata acttatttcc aactgaatta tcagatgaaa tagcaatacc
    14881 tcacacagat ggaatagatg taattaggcc agctataagt atagccatct taaagaagcc
    14941 tattgtgtgg agtaaaagta aagtaaaaat aatactttta ttagctgtag accaaaagtg
    15001 ttttaatcca ttaagcactc tactctcatt tatagaaaca gatgaattta agaagataaa
    15061 aaacattaaa gatagagatt ttattaggga gatgatatta gatggagtca atgaaaataa
    15121 ttagtagcaa tttaattttt aaaaatattg aagtgtctaa caatgaagat gcattaaagt
    15181 ttttgggaca acgtttgttt gacgaacagt atgtaaaaga aagctacata caagctgttg
    15241 tagcaagaga aaaaaagtat gctacaggat taccaacaga aatatacgga gtggcaatac
    15301 cacatacaga tatagtacat gtaaatgagc caggaatagc aattggaata ctgaataaac
    15361 cagtaaaatt tataatgatg ggtacagacg atactgaaat tgatgtaaaa gttgtattta
    15421 tgttagcagt gaaagagcca caagaacaat tgcaactatt agaaaaactt atgactatat
    15481 ttcaagataa gaatatacta aataatattg ttgacttaag tgaagaaagt gtgagtgatt
    15541 tattaaactc taaattgaaa aattaaacat gtaaagtata ttaaaaatgt aaaattaaac
    15601 atgcaaagta tgttaaaaaa tataaaaaga ttattaaaga tttctaacta tagatatctt
    15661 taaaataaaa taatttcaat gggggtgact aatgtgacta aaaagatatt agttgcatgt
    15721 ggaacaggag tatgtacttc aacaattgct ataaacaaat taaagaaagc acttcaagac
    15781 attggtaaat tggatatggt taatattaca caatgtaaag tagtagaagt tgcatcaaaa
    15841 gcacctgatt atgatttaat aatttgtact acacaagtat ctagtagtat aaaaacacca
    15901 gtgataaatg gattaccttt tttaactggt gtcggaatgg ataaattaat caatgatgta
    15961 ttagaagagt tagaattata attaaaaaaa ttaaggaaca tggtctctaa aatcataagt
    16021 tttagagact taaaattaaa taatggaggg tctttatatg gagatattaa atttcattgt
    16081 tggtctaggt gcacaagtaa tgatgccaat aataatatgt atttttggtt tgatattagg
    16141 tacaaaacta ggaaaatctt taagagctgg tttaactgtg ggagttgggt ttataggtct
    16201 taacactatt atagctttgc taacagataa tttaggtcca gctacacaac aaatggttaa
    16261 gaatttaggg ctaagtttat caataataga tgtaggatgg ccagcagcat cagctatagc
    16321 ttttgcatca acagtagggg ctttaattat tccaataggt cttgtggtaa atatagttat
    16381 gctaattact aatactactc aaactgttga tgtagacata tgggattact ggcattttgc
    16441 ttttacagga gctttagtgg caggggctac tcaaagtgta atgtggggag tatttgcagc
    16501 agtagcaaat atggtaattg tattggtaat ggcagattta actgctccag gtatagagga
    16561 atatttaggt atgccaggta tatctcttcc tcatggattt acacaggcat ttgttccaat
    16621 tgctatagta gtaaataaat tattagacct aataccaggt ataaataaga ttgagataaa
    16681 tgctgataca ttgcagaaaa aatttggatt atttggagaa cctttaataa tggggtctgt
    16741 aataggtgta ataattggta ttgctgcgaa atatgatata aaaggaatat tacaattagg
    16801 tgtaactatg ggagctgtat taatattaat tccaaagatg gcagcattat taatggaagg
    16861 attattacca gtatcagaag ctgcacagga atttattgag aagagattta aaaacagagg
    16921 caagatttat ataggcttag attcagcagt aggaataggt catcctgtta cattatcagt
    16981 tgcactagtg ttggttcctt tgactatact tatagcagct atattgccag gaaataaagt
    17041 tttaccattt gcagatttag cagtaattcc atttgcactt gtattaatcg taccaattac
    17101 aaagggaaat gtgtttagaa cattaataat aggaataatt ataattactt ctggattatt
    17161 aatcgcaact aacttagctc cattatttac ccaaatggct ttaaatgcta gctttaaaat
    17221 gcctgaagga gcaactatga tttcaagtat ttgtgatggt gcgaatccat taagttgggt
    17281 atttgtaaaa gttatgaact ataaagttat tggaggagta gtttttggag ttatagcttt
    17341 aggaatggct atttacaata ggaatagaat aataaatgaa aataaaaagc tatctataga
    17401 agaataaggt taaaacaatt ttattaagga gtgtataggt tatgaaaaaa tataaatatg
    17461 attctaaaag gtacttactc agtgttacaa taccaggatt ttttatgatt ttaatattga
    17521 tttatgcact gtttaataac tatataaatt ttaatttgaa tatatatagt ttgttaataa
    17581 ttgtctgtac ttatggtata ttcaatactt ttatatcatc aagtaatcct aaaaaaatta
    17641 ttattgacag caaatgtata cattttacct catttatgac tacacataag tatgaaatta
    17701 aagatataga aaagataaga ataagagaat tttataataa aaaaatttat ctaaaaatta
    17761 atgatggaga cctttttaaa ggtaggtatt ggataagaac aaatatgttt aataatagta
    17821 tagcattata tagttatttc attgagttgg aagagtgctt atatccagat tcactaagat
    17881 ttagaaataa aaaatttgag aataagaata tataaaaata aaaaattgaa ttgaagccta
    17941 acttaatctt ttttgataag atataagttt gaactttata ctaaattttt agaattttca
    18001 atcaagacaa taacataata gctaagcaat tagaacacaa ctaagatgta taaggcatgt
    18061 atatgatgaa tgggaggatt taattaatgt tattagagaa attaagaaaa gaagtattac
    18121 aagcatcttt agacctttta aactataatt tagtgacatt aacaggaggg aatgtaagtg
    18181 ggagagatga acaaactggc tatatagcaa tcaccccaag tggaatggat tatagaaatc
    18241 ttacaccaag tgatattgtt atagtagatg tagatggaaa tattatagat ggaaaatgga
    18301 aagcatcagt ggatttatca gaccacttgt atatatataa gcatagagaa gacataaata
    18361 gtataattca tacacattct acatactcaa gttgttttgc tattttaaat gaacctatag
    18421 aatgcgcaag tacaactctt gcaaatgaag tagggggttc agtaccagtt gcaaaatttt
    18481 caccaccaac atttaaaaag atgggaaaat gtgttattga agcaattggt gacaaaagag
    18541 catgtctttt agcaaatcat ggcgtaattg cagttggtcc atctgtagga catgctctta
    18601 cagccgctgt aatgttagaa gattcagcaa aagtatatta cttagcaaaa tcaattggca
    18661 caccagtgct actgcctgat gaagaaattc aaagagcgag agatgtattt tttaatgtct
    18721 atggtcagga taaataaatt agataaaatt ttaaaagtaa tatgaaaata tagatattca
    18781 atgatattaa ggagatttat aatgatataa taagtttaat agttaagaag taaaatatga
    18841 aatgtaatag tatataaatt catttaagac taaaaataat aagataaaat aaaaaggagt
    18901 ctgtatttct atgtattact tattaaacaa aagcacaaat ccttacttta atttggcatt
    18961 agaagaatat ctatttttaa atgataaata taatgatgat attataatca tatggagaaa
    19021 tgaagactcc atttttatag gtaaaaatca aaatccatat caggaagtac atcatgatgt
    19081 tattgaaaag ggagaaattc ctattttaag aagaatatct ggagggggca ctgtttatca
    19141 tgatttaggt aatatcaata tgtcattcat acaaaaagat aggcaacttc atgagataga
    19201 ttttttggag catactaagt ttatgcaaaa gatgctttca accttaggac tagatgtatc
    19261 tataactgaa agaaaagatt tatttttaaa tgggaaaaaa atttcaggct ctgcacagtc
    19321 tataaaaaga aaaaattctc tttatcatgg aactcttctt tacgattcag atttaaataa
    19381 attaactaaa tatttaaatt caaataaagc aactgaatca aatgcaacta aatctgtttc
    19441 aagtaaagtt acaaatatta agtttttact cgaaaaagat ataaattact ttttagatta
    19501 ttgtgttgaa tatttaaaat ctaatataaa gaatataaaa gaacttgaat tggatgaaga
    19561 agatattaaa agtgtataca atataggaga agataaatat agaaagatag agtggacata
    19621 tggtaaaacg ccgaaatttc aagtagtgct accatttaat aatgaaagta aaataaatat
    19681 acatgttaac agatggaaag tatcaaaatt ttttatttct tatgatgata atgtaattaa
    19741 tatggataag ttgttagatt tgaatttctt taaagaagaa ataaaagaga gtattttaga
    19801 aaattatcca cattacatag atttaatcaa tttaatattt taatataaaa cctctaattt
    19861 gataaaattg tcttatatca aactagaggt ttttaatatt cttgcatatt aacaaccatt
    19921 acaaaattgt aaaattaaaa acaacactat tgaaaaatca atatatattg taataaatat
    19981 atgtgttttt cattagcaaa tagacattaa ctatttatac aactgaaaat ttaatatata
    20041 attaatcaga tggttaaaat aaaaatatta aaagttttaa taaatgatgg ttaaaaataa
    20101 aatacgaggt gataccatgt acagtgttat aattgttgca gcgggaagcg gaaggagaat
    20161 gaacttagat ataaacaaac agttcataaa attaagagaa aaagaaatta ttgctcatac
    20221 aattcaagtt ttttatgaaa atataaatat agatgaaata gtggtatgta taaaaaaaga
    20281 agaagaggat ttttttaaag aaaatataat aaataagtat aactttaaaa atataaaaat
    20341 agcatatggc ggaaaagaaa gacaggactc aatatataat ggtttaaaaa aattagatga
    20401 aaattgtgat atagtactta tccatgatgg agcaagacca tttgtcgacc atagaataat
    20461 caacgaatct ataaaggtag ctaaagaaaa aaaggctgtg gttgtaggtg ttccagtaag
    20521 tgatactata aagatagtgt cagatggaac agtacaagaa actccagaaa gaaatttgtt
    20581 gtgggcagca caaacccctc aaacctttga atataatctc ataatagatg cttatgagca
    20641 agcatataag aacaattatt atggtactga tgattcaatg ttggtagaaa atataggtca
    20701 aagtgtcact atggtaatgg gttcttatga aaacataaaa ataactagtc cagaagattt
    20761 aaatatagca gaacaaatat taaatatgga aaaaagagat gaagttaaca gtagaaggag
    20821 aattatatga gaataggatt aggatatgat gtacacaaat taacagagaa tagaaaactt
    20881 ataatcggtg gtgtagagat accacacgat aaaggtttgt tgggtcattc tgatgctgat
    20941 gtattgatac atgcaataat ggattctatt ttaggtgctc tagcattagg agatattggg
    21001 aagcatttcc cagatacaga tgaagaatat aagggagcag atagtatgaa attattagaa
    21061 catgtgtata atctaataac aagtaaagga tataaaattg gtaatataga tagtactata
    21121 attgctcaaa gccctaagat ggctccatat attgagagta tgagaagtaa tatatcaaaa
    21181 gttttaaaca cagacattga taatataaac ataaaggcaa caacagaaga aggtcttggt
    21241 tttacaggag caaaacaggg aatagcttca caaagtattt gtttattatt gttgacaagt
    21301 cagaataatt aatatattat tagtaataag tttaatggat atcaagtaat aggcctatat
    21361 agaatatcta taatgtatga atttaagtga aattaatata aatataaaat tagctatgat
    21421 aaagaaatag taaaatgatg aaatcttcac agagagaaaa cggtggtgag agttttcaag
    21481 aggaagtctt tgaacctgtc ttttagctta aaggtataaa aatctttacg gtaggaaccg
    21541 ttatattccc aaaagagagc tattcatata gctaactaga gtggtaccgc ggaaattaaa
    21601 cctttcgtct ctatacttgt atagagatga gaggtttttt atattttcag gaagttaata
    21661 ttcaggagga atctattaat gaaaatgtca aaaatgttta tgccgacatt aaaagaaatc
    21721 ccagcagatg ctgagattac aagccatcaa ttaatggtta ggtcaggcat gataaaaaaa
    21781 atgacttcag gtgtttataa ccaattacca atggggttaa gagtgttcaa aaaaattgaa
    21841 caaataatta gagaagaatt aaataaaaaa gattgtcaag aaatactttg tgcagcctta
    21901 cttccttctg aattatggaa agagtctgga aggtggactg ctatgggtga agaaatgttt
    21961 agactaaaag acagaacaga aagagagtac tgtcttgggc caacacatga agaagcattc
    22021 actgatataa taagacaaga aattacttca tataaacaat tgccactgaa cttgtaccaa
    22081 atacaagtaa aatatagaga cgagagaaga ccaagatttg gtgttatgag aactaagact
    22141 tttacaatga aggatgcata tagttttgat gtagatgata aaggtttaga taagtcatat
    22201 caagatatgt ttgatgcata tgtaagtata tttgatagat gtggattgga gaatagccct
    22261 gtacaggcag attcaggagc tataggaggt tctacatcag ccgaatttat ggttaagtca
    22321 gaagttggtg aagatgaagt tgtattttgt agtggatgtg attatgcagc caatgtagaa
    22381 agagcagaat cttgtaatct agcatcacaa aaagaagaaa tgaaagaatt agaagaggtt
    22441 catacaccag gagcagctac aataaaagag ttagaagaat ttttaaaaac ttctccagat
    22501 aagtttgcta aaactttagt atatgaggca gatggaaaaa cagttgtagt cgttgtaaga
    22561 ggagatagag aagttaatga aatcaaagta tctaatgcta taggttctgt tatagaattt
    22621 gctcttgcta cagatgatgt agtgagaaaa gtaactaatg cagaagtagg atttgcaggt
    22681 ccaataggta taaatgctga ctatgtattt atagataaag aaattgttga acaaagaaac
    22741 atagttgttg gggctaataa aactgagtac catataaaaa atgctaacta tggaagagat
    22801 tttgaaggaa tagttggaga ttttagaaat gttcaagaag gcgaccagtg tatagtatgt
    22861 ggtaagccac ttgaaatagc tagaggtgtt gaagtaggac atatatttaa actaggaact
    22921 aagtactcag aatctatgaa tgcaaacttc atagacaaag atggaaaatc aaagcctata
    22981 gtaatgggat gttatggaat aggtgttgaa agaacagcag cagctataat tgaacaacat
    23041 aatgatgaga aaggtataat atggcctttg tcagtagctc cataccatgt tgtgatagtt
    23101 ccagcaaata tgaaaaatga agaacaaata tcaatagctg aaaatatata taatgatttg
    23161 caagctatgg gtgtagaagt tttattagat gacagagatg aaagaatagg tgtcaaattc
    23221 aatgattcag aattaatagg aatacctatg aggattacag taggtaaaaa tattaatgaa
    23281 ggtaaagtag aatttaaact tagacataaa gaagataaag agataattga catagaagaa
    23341 ataaatgaaa aagtaaaggc agagtttatc aggaataatg ttaggttagg ataataactt
    23401 aaataagata ttgtatagat aatcaaagtt gtactttata gacaccattt tatctatata
    23461 taaggatata aaatggtgtt tttttataaa acaaataaat gaggaggtaa ttgaaatggc
    23521 aaaaaatgaa aaacaatttg ttgaagaaat aacaaagatg gaagatgatt ttccacaatg
    23581 gtacacagat gtaataacta agactgactt agttgattat gcaccagtaa aaggttttat
    23641 ggttgtaaag ccatatggat atgcattatg ggaaaagatg caagaattta tggataaaaa
    23701 atttaaagaa actggacata aaaactgtta ttttccatta ttaataccag agagtttatt
    23761 aaacaaagaa gcagaacacg ttgaaggttt tgctccagaa gttgcatggg taacacatgg
    23821 aggaaataaa aaattagaag aaagattatg tgtaagacct acttcagaga ctataatatg
    23881 tacaatgtat gcgaaatggt taaaatcata tagagaatta ccatatcttt ataatcaatg
    23941 gtgctcagtt gttagatggg aaaaatcaac tagacctttc ttaagaacat ctgagttttt
    24001 atggcaagaa ggtcatacat tacatgaaac agcagaagaa gcacaagaag aaacaataca
    24061 acaattagaa gtatataaag cgttatgtga agaattactt gcaatgccag ttgtagctgg
    24121 tcaaaaaagt gaaagtgaga aatttgcagg tggtgaaaga acatatacta tagaagctat
    24181 gatgcatgat ggtaaagcat tacaatcagg gacaagtcac ttcctaggtc aacactttac
    24241 taaggctttt gatattactt ttgcagatag agaaggaaat ttagctaatc cataccatac
    24301 atcttgggga gcatctacaa gacttatagg tggacttata atgactcata gtgataacag
    24361 aggtttagta ttaccaccaa gagtagctcc aattcaagtt gttatagttc ctatagctgc
    24421 taaaaaagga aatgtaatgg agactgttga taaaatatat gctgatttaa aaactaaggg
    24481 agtagccgta gaagttgatg atagagataa ctatacaact ggatggaaat ttaatgagtg
    24541 ggaaatgaaa ggtgctccag taagagttga aattggacca aaagatatag aaaataatca
    24601 agctatggta tttagaagag atactttaga aaaggattct atgcctttag aaggattagc
    24661 tgatgctata tgtgatttat ttgatgttat acacaatgat atgtttgaaa aagcaagaaa
    24721 acatagagaa gacaatacat ctatagttga aaatatggat gagtttagaa aagctttaga
    24781 agaaaagcca ggattcataa agactatgtg gtgtggagat gcagaatgtg aagctaagat
    24841 taaggaagaa acaggagcaa ctataagatg cttacctttt gaacaagaaa acttaggtca
    24901 caagtgtgtg tactgtggaa aagaagcaga tagtatggta gtaatggcaa aagcttacta
    24961 aaatacattt aaataaaaac tggggtagaa agtatttaat actttatccc agtttttttg
    25021 ttttttatga gtaataaaat gtatttgtaa ctatgtcata agataagtca tagatactta
    25081 tattaaaaag aatataatag agaactattt ttaataatta catattaatg aaagcaaaat
    25141 atttttgtta tatagatttt caataaagca aacaataaat ataaatgaat attagtaagg
    25201 agtgctttta atttttagaa aataaatgac ttaaaagaaa gatttatgaa attaaaacta
    25261 ggattttctg atataataaa catgtgtatt tttaaataaa aaggatatta ttattagatt
    25321 tgattaaaaa taaacttgca aaacaatgta ctagtttaat attatagtta tattgaataa
    25381 aatatcagga ggaagtaaaa atgaatgtaa gagtaaggtt tgcaccaagt ccaacaggat
    25441 ttgtacatat aggtagtttg agaactgctt tatataacta tttatttgca aaaaaaatgg
    25501 gtggagaata tatattaaga gtggaagata cagaccaaag tagattagta gaaggtgcta
    25561 tagagaatat gcttaatgct atgaaatggg ctggtgtcaa ccatagtgaa ggtgttatat
    25621 tagatgatag tggaaaagta gtacaaaaag gagaatatgg tccatatatt caatctcaaa
    25681 gattagatat ataccaagaa tatataaagc aacttttaga tagtggaaaa gcttattatt
    25741 gtttctgtac aaaagaaaga ctggatgaag taagagatgc tcaaagagca gcaggagaaa
    25801 cagctaaata tgatggtcat tgtaagaatc tttctaaaga agaagtagaa gcgaatataa
    25861 aagcaggaat accatatgtt ataagattaa gattgccaga gaatcataca attaagttta
    25921 cagacttagt tagaggagat atggaattta atactaatga cctagatgac caagtattaa
    25981 tgaaaacaga tggatttcca acttatcatt ttgcagtagt tgttgatgac tatttgatga
    26041 aaattacaca tgttataaga ggagaagaat gggtatcatc tactccaaaa catgtttatt
    26101 tatatgaagc ttttggatgg gaagcacctg tatttgtgca tttaccaaat atacttaata
    26161 aagaaaagaa aaaattaagt aaaagacaag gtgatgtagc tgttgaagac tttaagaaaa
    26221 aaggatattt accagaggga ttagtaaact atgtagcatt agttggttgg tctcctgaag
    26281 ataatcaaga attgtttaca atggaggaat tagaaaaagc cttctctgta gaaagagtat
    26341 ctaaaagtgg tggagtattt gatactgaaa aattaaattg ggttaatcag cactatataa
    26401 aagatggtga tgatgcttat ctaacagact tagcaatacc atttttagta gaagatggat
    26461 ttataacaga agaagaagct actaataaat atgatttctt aaagagtatg atatcagtac
    26521 ttaaagagaa attacaatat gtaaaagaaa ttactgaaca tgcaagtata ttttttggag
    26581 ataaaataga ggtagaaact gaagagggaa gagagttctt aagattagaa catataccta
    26641 ctttaataga tgctttgaga gaaaaaatag aaaaaacaga ggtattaaat gctgagtttg
    26701 ttcaagctat gcttaaagaa atacaaaaag agtacaaaat aaaagggaaa aatttattta
    26761 tgggctcaag aataattttg acagggcaaa tgcatggtcc agatttacca aaagtaatgg
    26821 aagttttagg aaaagaaact tgcttaaata gaatagctta tgtaaaaaat aatatattat
    26881 agtcataaaa gtccgctaat ttagaagcgg actttttttg tgttagtatt tggtgaaaat
    26941 atctgcttaa gaaaatgaag gtatttaagg taataaattt aatatataaa aagcaaagca
    27001 aaaatataaa taaaatatga ataataaact aataaaatat taagcataga agaagggaat
    27061 ctattatttt agtttgattt aattattaag ggggtaatta ctatgtatag taatattgat
    27121 gatgttaaaa aggaattaaa ggagctatgc ttagaatatg ttacaatttt agaaaaatta
    27181 aaagatgaaa aaatgattac agaagaaact tttgaaaagt gctcttctca aaaaaaaata
    27241 tttttagaag aacaatagag gatggtaata gtattattat tatgtagttt gaaagatagt
    27301 acgaaaaatg atagtaatac tattatttat tgcaattttc aactataata taatatataa
    27361 tgttaagaag ataatttata aaaatacagt gaggtgatga gtgtgaaagt ttataataca
    27421 ttgactagaa caaaagaaga atttgttcca ttagaagaag gaaaggtaaa aatgtatgtg
    27481 tgtggtccaa cagtttataa ctatatacac ataggtaatg caagaccatt tataatattt
    27541 gatactctaa gaaggtattt agagtataga ggatacgatg taacttatgt acaaaacttt
    27601 acagatgttg atgataagat tataaataga agtcatgagg agggaatatc tcctgaagaa
    27661 gttgcggcta agtatataaa agaatatttt gtagactgtg atgggctagg gataaaaaga
    27721 gctactgttc atcctcaggt tacagataat atacaacaga ttatagagtt tataaaagag
    27781 ttggaagata agggatatgc atatgcagta aatggtgatg tatattttga tacaaataaa
    27841 tttgaaggtt atggaaaact ttcaggtcaa aaacaagaag atttagaggc aggggctaga
    27901 attgaggtta atgaccaaaa aagacatcct atggactttg ttctttggaa ggcaaaaaaa
    27961 gaaggtgagc caggatggga tagcccttgg ggagaaggta gaccaggatg gcatatagaa
    28021 tgttctgtta tgtctaagag atatttaggt gaaacaattg atatacatgc aggtgggcaa
    28081 gatttaacat tcccacatca tgaaaatgaa atagctcaaa gtgaagctag aagtggaaaa
    28141 atattctcaa aatactggat gcataatgga tatataaata taaatgatga aaagatgagt
    28201 aaatcaaaag gtaatttctt tacagtaaga gatatatcaa aattatatga tttagagata
    28261 gtaagatttt tcatgttatc agcacattat agaaatccag taaactttag tgatgagatg
    28321 ttaaatcaag ctaaagcagg tcttgaaaga ctatataata ctaaagaaaa attagaattt
    28381 acgctaagta atttagtaga atctccttta acagaaaaag aagtagagct tgttaaagaa
    28441 ttagatgact ttagacaaaa attcatagat gctatggatg atgatgttaa tacagcagac
    28501 gcagtaagtg taatatttga attagctaag ttaataaatt ctaatgtaga tgaaaattct
    28561 tctttggagt ttgctaaaaa atgcttagat gaatttaatg aacttacagg agtattaaat
    28621 attgtaaata agaaaaagga tactgtatta gataaagaca ttgaagaatt gatacaaaaa
    28681 agaactgatg caaagaaaaa taaagagttt caattagctg atgatattag acaacaatta
    28741 ctagatatgg gaattgtatt agaagacaca agacaaggtg ttaagtggaa aaggatataa
    28801 aaatggaaaa aactgaatta gttactatgt caccattagt tttagcatac ttaggtgata
    28861 cagtgtatga gacatatata agagaatatt taattagaca gaatacacaa agaaaagtta
    28921 atgatttgca taaactagct ataaagtatg taaaagctaa agctcaggca actataattc
    28981 atgaaattga aattgagtta actgaagaag agagtaaaat ttataaaagg ggaagaaatc
    29041 aaaagtcaaa tacttctcct aagaatgcag atattataga ctacaaacat gcaactgggt
    29101 ttgaggcatt agtgggatat ttgtatttaa ataatgaaat agagagactt caatatatta
    29161 tcaataaagg aatcaaaatt attgaaagag atatgtaaat attaaagagt gttgtcttaa
    29221 taataagtaa taaggcaaca ctttttttat aactaatgta taataaatat atagaaatta
    29281 gtaatgagaa aggataagaa agttatgaaa gtaaaattaa tatcacatac acctgaacca
    29341 gaaaaagtaa tagctatggc agctaagcta tgttattcac cagttgggac agacgaaata
    29401 gaaaaagatt tctaaaatat attaaagagc attcctataa caaatactat ggcggttgga
    29461 atctatgaga agttatagat atagaattat gtaaattatt tggaaaaaac aggtttgaaa
    29521 attagccatg ttggtatttc taggttactt aaggaagatc aaacataaat aagctgagtt
    29581 gtcttaggtg cactatgtac aacattagaa tgtgaagttg aagatttatt acttcaaacg
    29641 aacgatggta ctaagaaaaa caatgatgaa atgattaatt aataataaag gtaaggaggt
    29701 agtatatgag tttagcagat aaaattttta ttgatatgtg taataatatt cttgaaaatg
    29761 gagtatcatc agatggagag gtagttagag caaaatggga tgatggccag cctgctcata
    29821 caataaagaa gttttgtgta gtaaataggt atgatctttc aaaagaattc ccaatactta
    29881 cactaagacc aacaaactta aaagcagcaa tagatgaatt actttggata tggcaaaaaa
    29941 aatctaataa tataaatgat ttgaatagcc gtatttggga tagctgggct gatgaaacag
    30001 gttcaatagg gaaagcttat ggataccaaa ttggacagaa acataaatac aaagaaggag
    30061 aatttgacca aatagataga gtgatttatg acttgaaata taatccttat agtaggagaa
    30121 ttataactaa tatatataat cataatgatt taaatgaaat gaatttatat ccttgtgcat
    30181 atagtatgac atttaatgta actggaaata gattgaatgc aatattaaat caaagatcaa
    30241 atgatatttt ggtagctaat aattggaatg taactcagta tgcaatttta gttcacatgt
    30301 tagcacaaat aagtgatttg gaagtaggcg aattggttca tgttatagcg gatgcacata
    30361 tatatgatag acatattcct ctagttaaag agcttattaa tagagaatct tatgatgcac
    30421 caaaattaat aataaatcca aacataaaga atttctatga ctttagagtt gaggacttta
    30481 cattagaaaa ttataaaagt gggccacaaa tcaaaaagat tcctgtggca atttagaagg
    30541 agttatttat gaaagttata gtagctgtag atttaaattg gggaattggt tatggtggaa
    30601 agttactaca gtcgattcct gaagatatga aattttttaa agaaaaaact gtaggtaatg
    30661 tagttgtaat ggggagagaa acctttaaat cattaccagg aaaaaaagca ttaaaagata
    30721 gagtgaatat agtcttgact agaagtaaat cttttaatga tgatagattg attgtttgta
    30781 attctataga tgaaactctt aaggaactac aaaaatatga aaatgataaa atttttataa
    30841 taggtggaga aacagtatat aaacaatttt taccttattg taatgaacta tatgtaacaa
    30901 aaatacagaa gacatatgag gcggatagat ttttccctaa tatagatatt atgtcaaact
    30961 gggatttgat taaagaaagt gaagtaaaag aatataacaa tataagatat gttttttcaa
    31021 catatataaa taatagttta taggtttgta taggagagaa atttaattta tgaaaaagga
    31081 tatatttgta aatagagttt ctaatgcttt agaaaatgat agaggcgcag tttttgtagg
    31141 tagtggtata tcgtttagtt cgactgaagt tgattggttt aaattactgg agccattagt
    31201 aaatgaactt gatataacat tagacaataa aagtgatgat ttacctatga ttgctcagta
    31261 catagtaaat aattattcag gaaatagagg gcctttagta aacgaaatat caaaaacatt
    31321 taatagaaag tttaaactaa acaagtatca taagtattta gcaacaacta aactttcaac
    31381 aatatggaca actaattatg atatgttgtt agaagatgca ttttatgatt ttgatacatc
    31441 ggttaaagta agtgatgatt ctattacaag gaatagacta gaatatgatg tggaaattat
    31501 aaaaatgcat ggatgtattt ctagatccga ttggaatgaa atagttataa ctcaagaaga
    31561 ttatgatgat ttttcaataa ataaaccagc tatatctcaa agattaagtg aagatttaat
    31621 aaataagtct tttctattta ttggatatag ctatagagat ccaaatataa gaaatataat
    31681 gataaatgct agaaggctaa gtaaaaaaca tactcaacaa cattatataa ttttaaaaaa
    31741 gttaaaagat agtgatagta atattacgca agatgatgta gtaagacaag aactttggtg
    31801 caatgattta aaacgtttgg gaatttcaac tttatttata gatgaatact cagagttaga
    31861 ggaactttta agagagataa gtcaaaaatc aagaggtaaa acagtatatg tgacaggttc
    31921 tcataaagaa agcaatatga atgcaagaaa attaggaacc aagctagcaa aacataaaga
    31981 tattacttta ctaagtggtc aatcatctgg tattggctca gaggtagttg ctgcgtttac
    32041 tgaagagtgt attaagagta aagaagatat tataaataga atgaaaatat ttcctaatcc
    32101 atattcagct gatgaaaaat actctgatga tattacttta ataccagatt taaagaaatg
    32161 tagaactaaa atgttgaatt caacacaagt tgtagttgta tttagtggag gtataggtac
    32221 tgaagcagag atagatgttg ctataaaaca aaactgtaat ataattcctg ttataacatg
    32281 taaagaggat agagagaatc cagctataaa aaaaatctta aactatagtg ataatatgaa
    32341 aaatatagaa ataaaagatg ctacctatta taataaaata gtagatgaag atggtgttgt
    32401 aacaatggag gaaatttata tatgcatatc aaaaataatg aattaaaaca tgacattatt
    32461 aaattgaaaa aaagaaatgg atttattgaa gttggaaaag ataaaaaaac gttggtaaac
    32521 tgtttaattg gagcgaatga attagaggat tacaaatatg aaataaaaaa aatagattgt
    32581 atacttaata tatcagaaaa accagatata gtttcagatt taagtactaa aagaagaaaa
    32641 ataagtgaat ctatatggta taagatagtt aaagatacac ctttcgtatc tgctacactg
    32701 cccatatact tagttagtaa aaataataat aaaatagata gtaatgaatt attagatata
    32761 attattgagc agatggaaaa tggagttggg atgataacga ttcatccaac agcaaataaa
    32821 caaatttttg aggattctaa gaatagaatg gtacctgtca catctagagg tggaggtatg
    32881 gtaatacaag atttaatctc aaaaaatttt tcagaagata atgtgtattt gaaaatattg
    32941 ccagaaatta taaagtatgc tcaaaaacat aatgtttgta tcagtctagg tgcaacattt
    33001 agatcaggaa acatatttga ttctaatgat agagcacaga taacagaaat taagagtcaa
    33061 atagaattag ctaagaggat atataaaagt aatgtcgggg ttattattga atcaccaggt
    33121 cattcaagac caagtgacat aattaaaata tcgtcaatat taaaaaaaga aggtttccca
    33181 attatgccac taggaccaat accaacagat attgcaatag gaatggatca tatcgctgca
    33241 gctattggtg ctacaattat gggtatagaa ggatgtgcaa atataatatc aactgtcaca
    33301 cgagaagaac atactggagg gagaccttct attgaatcca cagttgaatc tataagaaca
    33361 gcaaaaatag ctgctcatat tattgatata catcaaatga atgatattaa cttagatatg
    33421 agtgtatcta agtctagagc taaatctaac acctgtatat ttgggaaaga tacaagatat
    33481 tgtgacagat gtagagaact gtgtcctttg tgcattagat aaaaattata ttcaagttaa
    33541 ttcaataaaa aagattggat aaaaatacat cgtatagcta gtttttcaca acagagtcaa
    33601 agatatgtca aattagagca atttgaatac ataattcctc cagaaataga aaaaatagaa
    33661 aaagcaaaag agttatttat aaattcaatg aaaaaatacc aagaggatta tgataaatta
    33721 gtagagatac tatttgaaaa tcattataac aaactaataa aaggtggtaa aaacgagaaa
    33781 acatcaaaaa gacaagttga gaagaaagca atagaagatg ctagatacgt atttccaaat
    33841 gcgtgtgaaa caaagatggt atttactata aatgctagaa gtttgtttaa tttctttgaa
    33901 catagatgtt atgaaaatgg gagttgtcta gaaggaacta tgacttgtgg agatatagtt
    33961 caagttagaa aaaaatttaa gaacttgtag gaggtgaata atttggcgag tatagaagga
    34021 agaaatccgg taatagaagc aataaaaagt gacagagaaa tagataaaat attaattgca
    34081 aattcagcta aagaaggttc aattaagaaa ataataggaa tggctaaaga taagaatata
    34141 attattcagt atgttgataa acataagcta gatgaagtaa gtacaagtca ttcacatcaa
    34201 ggtgtaatag cttatgcaag tgagtataag tattatgaat tagatgagct aatagattta
    34261 gcaaagaata aagatgaaga cccatttttc attatattag atgagataac agacccacat
    34321 aatttgggtt caataataag gacagcagat gcagtaggag cacatggagt tatcattcca
    34381 aaaagaaggt ctgtacacat aactcctgta gttgccaaag catctgctgg agcagtagaa
    34441 tatatgccag tttgcaaggt aaccaatata gttaatacaa taaaaagact caaagaagaa
    34501 gggctgtgga ttgctgcagc tgatatggat ggagaaactt tttataaaca aaatcttact
    34561 ggtcctcttg gtgtagtaat tggaagtgaa ggttttggaa tatctagatt ggttaaacaa
    34621 aactgtgact ttatagtaaa aatgccaatg ataggtaatg ttacatcttt aaatgcatct
    34681 gtagcaggtg gaatactttt atatgagata ttcagacaaa gactggataa aagtaaatag
    34741 gcatgaaaaa aaatataaat cattacttaa taatagatgg ctacaatatt ataaatgcgt
    34801 gggacaattt gaaagaactg gctaaagagg acttagaaga ttctcgagaa aagcttattg
    34861 atgacattat agaattttct gagtttatgg gttataagac tataatagtt tttgatgctt
    34921 ataatgttaa aaattctcga gaaaaagttg aaaaaagaaa acacataaca atagtttata
    34981 caagagaaca ccaaacagca gatagttata tagagaaatt tataacttct ttatcaaagt
    35041 atgatgatgt aaaagtagcc actaatgact atgcagaaca gcaaatgatt ttaggaaaag
    35101 gtgctactag aatgtctgca agagaattaa agttagagtt agatcgttcc aagaataaaa
    35161 tgaaggaaaa aaatatcggt ttgaggaaaa aaattcagcg aaattggttg gaggaaaggt
    35221 tagataaaga aacattgtcg aaacttgaga acattcgtag aaagcgttga aattactagg
    35281 ttctttagac tataatatac ctataaattt gttttatata tgggggagat ttacatgttg
    35341 gtagcaaaag aaaaaagtta tgagttagtg gacaattgtc agcaggatga gtataacatt
    35401 gtattaagag caagtgaagg ggataaaata gcactagagt acattattac taaatacaga
    35461 aattttgtaa aggcaaaagc aaagtcatat tttttaattg gagcagataa agaagatata
    35521 atacaagaag gaatgatagg cctttataag gcagttagag attttgatgg aagtaagacc
    35581 aattcattta aatgttttgc ggagatatgt ataacaagac aaataataac tgctataaaa
    35641 actgcaacaa gacaaaaaca tataccttta aattcatatg tttctttgaa taaaccaata
    35701 tatgatgaag agtcagacag aacactttta gatattatag ctacaagtat agtaacagat
    35761 ccagaagagc ttataattag taaagaagag ttaaaaaata tagaatcaaa aatgaacgaa
    35821 ttgttaagtg acttggaatt agaagtacta gaattatatt taaatggaaa gtcataccag
    35881 tttatagcgg acaagcttaa aagagatgta aagtcaatag ataacgcttt acaaagggtt
    35941 aagagaaaat tagaaaaaca tcttgaaaac agaaatgatt aatagtaaaa tacttaccat
    36001 aagcttattg tgagcaaaat taatttatat cgaaagataa gggaggaaat tcaaaatggc
    36061 taaagctaaa tacgaaagaa caaaacctca tgttaatata gggacaatag gacacgtaga
    36121 ccacggtaaa actacattaa cagcagcaat aacaaaaaca ttatatgaca gatatcaatt
    36181 aggagaagca gtagatttcg caaacataga taaagctcca gaagaaagag aaagaggaat
    36241 cacaatatca acagcacacg ttgagtatga aacaccaaat agacactatg cacacgttga
    36301 ctgcccagga catgctgact acgttaagaa catgataaca ggagcagcac aaatggacgg
    36361 agcaatatta gtttgttcag caacagatgg accaatgcca caaacaagag agcatatact
    36421 attatcaaga caagttggag taccata
//
LOCUS       DAELSY010000023        36018 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.023, whole genome shotgun sequence.
ACCESSION   DAELSY010000023 DAELSY010000000
VERSION     DAELSY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36018
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.023"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            113..553
                     /locus_tag="KPG09_001037"
     CDS             113..553
                     /locus_tag="KPG09_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0965660.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            569..2020
                     /locus_tag="KPG09_001038"
     CDS             569..2020
                     /locus_tag="KPG09_001038"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBG0965661.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGSFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAIETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(2111..2779)
                     /locus_tag="KPG09_001039"
     CDS             complement(2111..2779)
                     /locus_tag="KPG09_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBG0965662.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSQYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
     gene            3048..3188
                     /locus_tag="KPG09_001040"
     CDS             3048..3188
                     /locus_tag="KPG09_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965663.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(3363..3683)
                     /locus_tag="KPG09_001041"
     CDS             complement(3363..3683)
                     /locus_tag="KPG09_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG0965664.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(3803..4540)
                     /locus_tag="KPG09_001042"
     CDS             complement(3803..4540)
                     /locus_tag="KPG09_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBG0965665.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            4755..6578
                     /gene="acd"
                     /locus_tag="KPG09_001043"
     CDS             4755..6578
                     /gene="acd"
                     /locus_tag="KPG09_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBG0965666.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYIS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            6793..11091
                     /locus_tag="KPG09_001044"
     CDS             6793..11091
                     /locus_tag="KPG09_001044"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBG0965667.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEILPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            11295..11756
                     /locus_tag="KPG09_001045"
     CDS             11295..11756
                     /locus_tag="KPG09_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBG0965668.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            11786..12940
                     /gene="nusA"
                     /locus_tag="KPG09_001046"
     CDS             11786..12940
                     /gene="nusA"
                     /locus_tag="KPG09_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBG0965669.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVKSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELGTSEDNFSEEE"
     gene            12947..13228
                     /locus_tag="KPG09_001047"
     CDS             12947..13228
                     /locus_tag="KPG09_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBG0965670.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            13218..13529
                     /locus_tag="KPG09_001048"
     CDS             13218..13529
                     /locus_tag="KPG09_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBG0965671.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            13551..15491
                     /gene="infB"
                     /locus_tag="KPG09_001049"
     CDS             13551..15491
                     /gene="infB"
                     /locus_tag="KPG09_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBG0965672.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            15534..15914
                     /gene="rbfA"
                     /locus_tag="KPG09_001050"
     CDS             15534..15914
                     /gene="rbfA"
                     /locus_tag="KPG09_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBG0965673.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDNLIKRVNEKNAQQNEDENYDE"
     gene            15895..16878
                     /locus_tag="KPG09_001051"
     CDS             15895..16878
                     /locus_tag="KPG09_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBG0965674.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNVGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEGVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQTKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSDYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            16891..17796
                     /gene="truB"
                     /locus_tag="KPG09_001052"
     CDS             16891..17796
                     /gene="truB"
                     /locus_tag="KPG09_001052"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBG0965675.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEVVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            17927..18856
                     /locus_tag="KPG09_001053"
     CDS             17927..18856
                     /locus_tag="KPG09_001053"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBG0965676.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEYAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            18960..19217
                     /gene="rpsO"
                     /locus_tag="KPG09_001054"
     CDS             18960..19217
                     /gene="rpsO"
                     /locus_tag="KPG09_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBG0965677.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            19457..20302
                     /locus_tag="KPG09_001055"
     CDS             19457..20302
                     /locus_tag="KPG09_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG0965678.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            20427..22538
                     /gene="pnp"
                     /locus_tag="KPG09_001056"
     CDS             20427..22538
                     /gene="pnp"
                     /locus_tag="KPG09_001056"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBG0965679.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            22804..23547
                     /locus_tag="KPG09_001057"
     CDS             22804..23547
                     /locus_tag="KPG09_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0965680.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            23705..24952
                     /locus_tag="KPG09_001058"
     CDS             23705..24952
                     /locus_tag="KPG09_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG0965681.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAICLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            24987..25232
                     /locus_tag="KPG09_001059"
     CDS             24987..25232
                     /locus_tag="KPG09_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG0965682.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            25242..26465
                     /gene="dapG"
                     /locus_tag="KPG09_001060"
     CDS             25242..26465
                     /gene="dapG"
                     /locus_tag="KPG09_001060"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG0965683.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEENIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            26578..27312
                     /locus_tag="KPG09_001061"
     CDS             26578..27312
                     /locus_tag="KPG09_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBG0965684.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            27435..29846
                     /locus_tag="KPG09_001062"
     CDS             27435..29846
                     /locus_tag="KPG09_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBG0965685.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKSNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKSSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            29849..30907
                     /gene="mnmH"
                     /locus_tag="KPG09_001063"
     CDS             29849..30907
                     /gene="mnmH"
                     /locus_tag="KPG09_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBG0965686.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESVFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            30901..32235
                     /gene="rimO"
                     /locus_tag="KPG09_001064"
     CDS             30901..32235
                     /gene="rimO"
                     /locus_tag="KPG09_001064"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBG0965687.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFSELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            32222..32764
                     /gene="pgsA"
                     /locus_tag="KPG09_001065"
     CDS             32222..32764
                     /gene="pgsA"
                     /locus_tag="KPG09_001065"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBG0965688.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            33052..34098
                     /gene="recA"
                     /locus_tag="KPG09_001066"
     CDS             33052..34098
                     /gene="recA"
                     /locus_tag="KPG09_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBG0965689.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            34274..35815
                     /gene="rny"
                     /locus_tag="KPG09_001067"
     CDS             34274..35815
                     /gene="rny"
                     /locus_tag="KPG09_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBG0965690.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT        14613 a         3788 c         6833 g        10784 t
ORIGIN      
        1 aagttgatat atagtataat tataaaaaga aaagaagtat tatattatag atatataaac
       61 gcatttgaga tataagaggg agttataaat cattagtttt gaggtgaatg ttatgagcga
      121 taaagaatta tctattttgt tgttagaaag tatgaataat tttcataatt taataaagat
      181 tattaataac gaaagatata aaaaagataa ggttcttaca gaaagacagt tctttgcctt
      241 agtaaaaata agaaaacatg ataaaataga gcttaaaaat ctaagtagag atttacatgt
      301 atctacttct agtctgtgta tactacttaa taaattggtt gagcaagagt atgtatatag
      361 ggaagaagat agtagagata gacgaaatac tttttatgga ataactaaaa atggagaaaa
      421 gatattggat aatgaaatac taaagtttgt atctataata agtgataaaa tggattgctt
      481 agatatagat aataaagata aattatttac ttctttggaa gagtctaaaa atataataga
      541 acaattattt taaaaaggag aatttattat gaatataaag tattctaaag agatattaaa
      601 aagtttagaa aatgctataa cagtagtagc tctatttgag ggagaagatg tttctacgac
      661 atttgataaa ataaatatga taagcagaaa ttttaattct aaagaaaata gtatagatac
      721 tgtaacattg gttgaaaata acaacttggt aaatgtttgt cttgttggtt ttggtaagag
      781 agagttattg aatagagaaa aaattagaat cataggtggt aatttgtgta aaaaattaaa
      841 aaatatattg aaaaatgaag catataagag tagtgatgtt aatgtactta atcttaattt
      901 aaatagtgat gaaataggtt cttttgtaga agggatatta cttggaaatt ataagtttga
      961 taagtataag acaaaaagta aggattttga aagaaaagaa gttgatacta taactatatt
     1021 tactgaaaaa gaagttgagt gtcaaataga aaaggctaaa atattagcta attcaactat
     1081 aatagctaga aatctagtta atgaaccatc aaacgtaata tatccaaaaa cattagcaat
     1141 agaaacagtt aaacttggtg cagagttcgg gtttaatgtt gatgtttatg aagaagaaaa
     1201 aataaaggca ttgggaatgg atgctttttt cgccgtttca agaggttcaa tcaataaacc
     1261 aagattcata gtaatgagat actttggaga taaagaaagt acagatatat taggtctagt
     1321 aggtaaagga ttgacttatg atactggtgg atattcttta aaatcaaatg cttctatgct
     1381 agatatgaag acagatatgg ctggtgctgc gagtgttata ggtgctatgt gtgctatatc
     1441 tcaaagtaaa ttaaagaaaa atgttatagc agtagtggct gcatgtgaga atgctttatc
     1501 aggaggttca tacaaacctg gagatataat tagttctatg gcaaagaaaa ctattgaagt
     1561 tttaaatacg gatgccgaag gtagacttac attagctgat gcaatatact acattataaa
     1621 caatgaaaaa gtaacaaagg tagttgatgt agctacatta actggtgctg ctttaacatt
     1681 gttaggaaat gttgcaactc ctatagtaac aaataatgat gatttttatt gtgaattaga
     1741 aaaagctgcc actttatcag gagaaagggt atggaagatg ccaatttatg atgaatttaa
     1801 agatatgata aaaggagaag aagcagacct taaaaacact ggtggtaaaa atgctggatg
     1861 tataacagca ggagctttta ttggtgaatt tgttgggaat actccttgga tacatatgga
     1921 tatagcgggg acaagtacat catcaaaatc aattggatac aaagcaaagg gggctacagg
     1981 agaacctgtt agaacattat actatttggc agaaatctaa aaaacaatta taaaatataa
     2041 tttataaagc cacatggata tttttgtaat ttaagattat ctatgtggct ttatttttta
     2101 tttaagtaaa ttatttactc tttttctttt tctcaagtat aaaagtatct ctattaaata
     2161 aaagagcagg tatgtacata atactagtaa caaaaataaa cgtaaaaata gcagttgtgc
     2221 tatgacttaa aaaaccataa tagtattgtg agttggtaaa tgtgtttagt aaaaatatga
     2281 atgaaaaata aataaaacat atagctaagt tatctctaag tgcatatctg ttaactctaa
     2341 attttctaac atcaaccata tatatatctg cataaattat caagcactta gagaagataa
     2401 atataaaata tattaagcta ggaaattcat agcccaagtt tttagaaaaa atgagttctc
     2461 caactgaaca aactaaactc caagaaaaaa atatattaaa aagatgatac tgcttaaaaa
     2521 ttagtatggc tatacacaga tatatacaga tataattgat tccaattggt aaagagttta
     2581 gagtactata attacctgaa gaaataagta ataattgctc tagagccata attattacaa
     2641 gtaatgtaca aattatcttc tcaactgtat agctataagg taaaatattt ttggttagtt
     2701 ttggacaata gtataaaaat aatccaaata taaagagact tacaaattgt tcactagaaa
     2761 aaagaaaaat tttaaacata taacattctc tcctataata aatttaatat gtatctatat
     2821 aaatatattc attatatctc aatttaatat ataaaaaaga taataatagt tagacttttt
     2881 atatgaattt taataggtaa aatatcctat tgttttgtag atatatccta attatgtcaa
     2941 aaaataaaaa aatttttatt tattagagtt tttggaaata ataataacag atattataat
     3001 taattaacag tttttgtaag tatttgacaa tagagagggt gtaacaaatg ggaataatag
     3061 agtcagcatc aaaattggct gagatggttc atttattggc tgttgaaaag gggataactg
     3121 atatagaagc atgggatgaa gctgtgaaag aatacagcaa aatttatgaa gaaagaagaa
     3181 atgaatagaa tgataaaaaa tgaaaacttg atgataaaaa ataactttat ttaaagaaat
     3241 tcaattttta tagaaatatt aaaaataata taaataaaaa gatggtattt caaatgtaag
     3301 taaaaaatca ttttgggata ccatccttat tatttatttt aataatatat aagtaataaa
     3361 tcttacttat ccatattttt tatgtaacct cttttttcta gcttttttat atgctcctta
     3421 cgtatttcat caagattaat accatattgt tcttctagct taaacatcat agtaacacat
     3481 gtctgagcta catccattag ttccttagca gttttcttta taatatcaac ttcatctaaa
     3541 tcattatttt ctccacttag atttctgaat ttacctatac attcagctaa ttcaccttgt
     3601 tcttcaccca ttttgataaa tgtagattct agtgttgggt ctaaattatt aagctctggt
     3661 aaagatatag tttttagttt cataaaagct cctttcaagt tattcgtatt tatactaaaa
     3721 catcataaca aaaataatac taaaaaaaaa ctttaaattc aacataaaaa cttacatata
     3781 taataaaaat cgtgaaataa atctatattt gtcttaatac agcctcatat aaaacttctc
     3841 caataggttt atttattaca agagaagctg acttatcata gactgtattt gctttattaa
     3901 taagagcaat ataatcacct ttgtaataat ctataaaact tgctgctggg tacacagcta
     3961 atgatgtacc tcctataatc aataaatcag cattacttat agcagaaatt gttttagtaa
     4021 tgacatcact gtcaagagct tcctcataaa gtactacatc tggttttact atagaaccac
     4081 agttgtcaca ataaggaata ttacctccca gatttaacat agattctaag tcaaaaaact
     4141 taccacattt agtacaataa tttctatgta cagaaccatg aagttctagc acatttttac
     4201 ttccagccat ttgatgaagt ccatcaatat tttgagttat gacagcttta agcttaccca
     4261 tttcctcgag ttttgctaaa gctatatgag cattgttggg ttttgcatta ggatatatta
     4321 atttatcctt ataaaattca aaaaattctt ctggatatct aacaaaaaaa gtatgtgaaa
     4381 ctaattgttc agcagtaaat tgtttattga gtttttggct aaataatcca gtagaacttc
     4441 taaagtctgg aatattagat tcagttgaaa cacctgctcc accaaaaaat acaatattat
     4501 tgtgattagc tattaagtct tttaagctat ttgcatccat agttacacct ccaaattatt
     4561 atatataaat tgtactacat attaggtctt ttatgtttta ttaatttaaa taaatatgta
     4621 gtttaataag ttaaataaag ggaatataaa taaaatgtat acagagtata aatagacaat
     4681 atttactaaa ataacaagtt gtcgtataat tatattaaaa ttgttttagt gtataagaag
     4741 ggcgggggaa gtacgtgaag aaaaaagcag ctttagcaac attggctatg ttacccttag
     4801 gtgtggtaaa tgcacatgct gatggagata taggtatagt gactataaat tatttaaatg
     4861 taagaaatga gccaactgcc gaaagtagca tagcctttgt tgctaaaaaa gatgataaag
     4921 ttttgattaa agactcttct aatggatggt ataaaataaa agctgaatct ggacaagaag
     4981 gttgggcttc atcaaaatat atagcaaaat caaatagtga ctctttaaga acgtctacaa
     5041 ataaagaaaa acaagttatt tcaaatagtt taaatatgag aaatggagca ggtacaagtt
     5101 acagagtaat aactgtacta aaaaaaggtc aaaaagtaga agtaatatca gagagtaatg
     5161 gatggtctaa aatcaaatat gatggaagac taggatatgt atctagctct tatttaggag
     5221 acgtttcaaa ttcaactaat aaatcaaaaa ctaaacaagt gaatacaact tcactaaatg
     5281 taaggagtgg accaaataca agttatggtt tgttaggcaa gttgccaaaa ggaagtaaag
     5341 tagaagtaat atcagaaagt aatggatggt ccaaaataaa gtataatgga aaagatgcct
     5401 atatatctag tatgtactta tcggatgtaa gtcaaagtaa ttcagacaat tctagtcaaa
     5461 gcaatgataa aaagaatact gataaggttg taaatacagc ttctttaaat gtaagaagtg
     5521 gaccaggttc tacatatagt aagttgggaa aagtttataa aggaagtaaa gtaactgtac
     5581 tatcagaaag tagtggatgg gctaagatta attttaacaa taaagaagca tttgtagtag
     5641 gcaattattt atctacttca gcagatactt caaataataa ctcaaatagt aactctgata
     5701 acagttctaa tagtaatggc aataattctt catcatcagg tcaagtaaat ggtatgtcag
     5761 gtataagtgg ggctaaaatt gattataaat ctttgagtta tactttagaa tctcatataa
     5821 gtaaacaagt tgagaaagca gcatcaggag gaaatgtgat agctccaagt aataggaaaa
     5881 gcactccaag tcctgaattt agtacttttt cagctcaaag aacaagctca tttgtaaatg
     5941 caagttcaag tgatatagaa tattatttaa atcctaaaaa ttttacaaat actactaaag
     6001 gtatgatgca gtttttaaag attaacagtt atagagatgg tatttcagaa tcaagtctta
     6061 actcgtatct taatggttta tcttctagtg tatttaaaaa ccagggagca gcctttataa
     6121 atgctgccaa gaagtataat atagatgttg tatatctagt atctcatgct atgtgggaaa
     6181 ctgcttatgg taagtctaca cttgcccaag gtcaaacatt aacatcttat aagggacaac
     6241 ctcttagtaa gccagttaaa gtatacaatt tttttggtat aggagcaata gataaaagtg
     6301 ctaatgtttc aggtgcagaa gcagcgtatt ccaatggctg gactagtgta gaagctacaa
     6361 tagatggctc tgcaaagtgg atatcacaaa attacgtaaa tagttctaag tacaatcaga
     6421 atactatcta caagatgaag tggaattatg attatacatg gcatcagtat gcaactgatg
     6481 taaactgggc taatggaatt tctggaatta tggaaaattt aattggtctt tatggaggag
     6541 gaagtagttt ggtttttgaa gttcctcaat ataagtaata cataaataaa aaatatagac
     6601 tatgggtagt tttcatttta tttaaattat ctatagtctt tttgtatata atttataatc
     6661 tgtaaattta atgttaacaa aacttttatg ttataatatt aattctaagt aaatataagt
     6721 gagtaatagg tagaaataga taaaaagtgg taataatata tataaagata attttgagaa
     6781 aggaggatat atatggaaag tattaaggaa tatttggata agttagaaat taataatagt
     6841 ggtcttggaa agcaacttaa agaagtttat ataaatagag tagtttattt taaggaagat
     6901 aagatagttt atttttatct aacttctaaa gatattgttt ctcatgaact tttagataaa
     6961 tttaaagaag aattgatgta taaactcgat tattttaaag atatgaggat gaagataaga
     7021 tttactggtc ttgaaagaaa atccaataaa gatgttataa aaaaatattg gaataatata
     7081 ttgtatatat taaagtatct ttgtccatct attgcagggt ggtataaaca agtagaattt
     7141 ctttgtttag aagaagaatt aaaaataaaa cttccaaaag gtatatttta tgagcgatta
     7201 atgaaaaaaa atgtaactta tgtacttaaa accgtgctta gcgaagagtt gggattagat
     7261 ttaaatataa caatagaaaa agctgttgat gaaaaggtaa ataaagaaag actcataaga
     7321 atcaatgata gagaaatgga agaaaaaatt agggctttag aaattggaaa agttaataac
     7381 tgtgaagaaa atgaagagga aagctatgtc attaagtctg atgtagatga aaatttaatc
     7441 tatggagata atgcaaatgc tatgattgaa aatatagttg aattaaatgc atcttctgga
     7501 acagttgcag ttgttggaga tatatttgat gttgacacta aagaacttaa aaacggaaaa
     7561 atacttatga tagcttcaat tacagattat actagctcaa taagctgtaa acttttttta
     7621 acagatacaa ataaggatgg agtacttgaa aatgttaaaa agggagcata cttaaaaatc
     7681 aagggtgaca tagtttatga tacatatcaa agagaaatta gtatgatgat tagtgggata
     7741 agaaaagaaa ctagaataga gagagaagat aaatcagagg aaaaaagggt agaactccat
     7801 gcacatacac aaatgtcttc tatggatgct atttgttctg ttaaaaagct tgtagagcgt
     7861 gcagccaaat ggggtcattc tgctattgct ataacagacc atggtgttgt acaaggattt
     7921 ccagatgcta tgaatgcagg taagtcaaat aatataaaaa tactttatgg agtagaaggg
     7981 tacttggttg aagatgattc tctcattata gaggatgcaa atgataaaga actttcgcag
     8041 acatttgttg tatttgacat agagacaact ggtttttcta atacaaatga taaaataact
     8101 gagattggtg ctgtaaaaat agaaaacttt aagattgtag atagatttag tgagttaata
     8161 aatcctgaaa aagatatttc ctataagata caggaattga caggaataac taatgaatta
     8221 attaaagaca agccaaccat tgaagaaatt cttcctaagt ttatggaatt tgttggagat
     8281 agtgtattag tagcacataa tgcagagttt gataccggat ttatatcgca aaaatgtagg
     8341 gaacaaggct tgttatataa taacaaaaaa gtagatacac ttatgttagc aagagtaatg
     8401 ttgccaaact taaaaagaca tagattaaat gtcgttgcaa aagaacttgg tattcctctt
     8461 ttaaatcacc atagagcagt ggatgatgct gaggctacag cacttatatt taataagttt
     8521 ttgcagatgc ttacagaaaa aggtgcaaaa actctaagtg atgtaaacaa tatacttgga
     8581 aagattgatt acacaaagct tagcacaaat catataacct tgatagcaaa aaattctata
     8641 ggtataaaaa atttatataa aataatttca gatgcacatg taaatcattt ttttagagca
     8701 ccaagaatcc ttagaagtgt attaaatgaa tataaagaag gattaataat tggttcggct
     8761 tgtgaagctg gggtggtttt tcaagctgta aagaaaaacg tgtctgatga ggaaatgaaa
     8821 aaaataatag atttatacga ttatattgaa gtaatgccta tagataataa tagatttatg
     8881 attgataaag gagaagtaaa ggatgaagaa gagttaaggg agttgaatag gaaattaatt
     8941 gatacagcca aaaaatttga caaaattcca gtagctactg gggatgttca ttttttagac
     9001 aaacatgaag ctgtcttaag aaaagttctt aaatattctc aaggttttaa agtagatgaa
     9061 gaagaaactt atttacattt tagaacaaca gatgaaatgt tggaagagtt tagttatctt
     9121 ggagaagaat tggcatatga agttgtagtt gaaaatagta atctgattgc cgatatggtt
     9181 gaagatataa aacctatacc agatgatact tatcctccaa ttatagaggg ctcagatgtg
     9241 gagttaagag aaatgtgtta caaaaaagcc aaacgaatct atggagaccc aataccagaa
     9301 atagttcaaa aaagactgga tagagaatta aattcaataa taagcaatgg gtatgcagtt
     9361 atgtatatta tagctcaaaa actggttgca aaatctcttt cagatggata tttagttggt
     9421 tcaagaggct cagttggctc atcttttgca gctactatga gtgatataac agaagttaat
     9481 cctcttccag cacattatat ttgtgaaaac gaagactgta aatattcatt cttttatgaa
     9541 ataggtgaat ggggctcagg tgttgattta ccagacaagg attgtccaaa gtgtggaaga
     9601 aaacttgcta aaaacggtca tgatattcca tttgaggttt tcttaggttt tgaaggtgat
     9661 aaggaaccag atatagactt aaatttctca ggaaattatc agccaacaat tcataaatac
     9721 acagaagaac tatttggaga ggggtatgta tatagggctg ggactatagg tactgtagca
     9781 gaaaaaacag cttttggata cgcaagaaaa tatgtggaag aaaataatat aagtgtacca
     9841 aatgcagaag tacttaggct ttcaaatgga tgtactggag taaaaagaac ctcaggacaa
     9901 catcctggtg gagttatggt tataccagac tataaagatg tatacgattt tacacctata
     9961 cagtatccag caaatgatac ttcttgtggt gttataacta ctcactttga ttatcattca
    10021 ataagtggta gaatacttaa acttgatata cttggacacg acggtcctac cattataagg
    10081 atgcttgaag atatgactgg aataaatata acagaaattc ctttagatga taaagagact
    10141 atgtctctat ttacatctac agaagcatta ggtgttacac cagaagaaat aaactgtcct
    10201 ataggttgcc ttgctgtacc agagtttgga actaagtttg tacgacagat gttacttgac
    10261 accaaaccca ctacatttgc agaacttgtg cgtatatctg gtctctctca tgggacagat
    10321 gtttgggtaa ataatgcaca agacttagtt agagaagata tagttggatt aaaagatgtt
    10381 atatctacac gagatgacat aatgaactat ttaatattta aaggattacc tcccaaaatg
    10441 agttttacaa taatggagag cgtaagaaaa ggtaaaggtc taaaaccaga acacattgaa
    10501 gaaatgaaaa aaaataatgt tccagaatgg tatataggct catgtaaaaa gattaagtac
    10561 atgttcccaa aagcacatgc tgttgcttat gtaatgacat catttagaat tgcatattgt
    10621 aaagttcact atccagaagc attttatgcg acttatttta cgacaaaagt tgaggatttt
    10681 gatgcagatt taattgttaa aggattagat gctataaaat caaaaataaa tgaaattgag
    10741 tcactgggaa atgatgcaac cacaaaagaa aaaggtatgt tgaccgtttt agaagttgct
    10801 cttgaaatgt atgctagagg aataaaatta ttaccagttg atatatataa atcagatgca
    10861 actgagttta tagttgtagg agaaaaaaca ttacttccac caatggcagc aatacaaggg
    10921 cttggaggaa atgcggctat aaacatccaa aatgaaagaa aaaatggtga atttatatca
    10981 aaagaagatt tgagaaagag aacaaaaata tcaaaaacgg taatagaaac tcttactaat
    11041 catggttcac ttgagaatat gagtgagaga aatcaattgt cattgtttta agttagccca
    11101 aaaagttgca aataaaagaa ttttatgata tcatagtata gaaaatagta tactttgcag
    11161 aaactaagag tgagacagct gttctcactc ttttatttta aatatattat taaaaagttg
    11221 gtttattttt attaaatata ttttatttaa gattatataa actaatcatt tacaattgaa
    11281 taggaggaac tttaatgaaa aaaaatttag aagctacaat tgaggagatt gtaactaaaa
    11341 taacggatga acatggattt gaaatggttg atgttgaata tgtaaaagaa gcaggagaat
    11401 actacttaag agtatacata gataaagaag aaggaatatc tttaaatgaa tgtgaattag
    11461 ttagtagaga attaagtcca atactagatg aaaaagaccc tataaaagaa aattactttt
    11521 tagaggtatc ttctccagga cttgatagag cattaaaaaa agacagagat tttgttagat
    11581 atcaaggtag agatgtagat ttaaaattat ataaaccatt aaatggatgt aagcaatttg
    11641 aaggtgaact tgtaggatta acagaagata ataatataaa aatcattgca aatggtaagg
    11701 aaatggaatt taatagaaaa gatgttgcta ttgtcagatt agcaataaag ttttagtggt
    11761 atattaaata gggaggatat aaaaaatgaa tcatgaattt atggaagcat tagatgaatt
    11821 agtagttgac agaggtatag ataaagaaat acttatagat actatagaac aagctctttt
    11881 aactgcatac aagaaaaact ttggttcagc acaaaatgtt agagttgaat ttgatagaga
    11941 caagggagat ataaaagtat tttctcaaag agttgttgtt gatgaatcag atttatatga
    12001 tactttctta gaaatagagt tagcggatgc tagagagata aaccctaatt atgaattagg
    12061 tgatattata gaacatgaag ttacacctaa aaactttgga agaattgcag ctcaaacagc
    12121 aaagcaagta gttgttcaaa gaataaggga agctgagaga gaaatagtgt ataatgaatt
    12181 tatagaaaaa gaaaatgaaa tagttacagg agaggttgct agagcaaata aaaatgttgt
    12241 ttatgtaaat cttggaagaa tagaaggtat aatgactcaa agtgaacaaa taccatttga
    12301 gaattataaa gcaggtcaaa aaataaaatt ctatatactt gaagttaaaa agactaacaa
    12361 aggacctcaa atagttgtat caagaagtca tccgggtcta gtaaagagat tgtttgaaga
    12421 agaagttcct gaaatatatg aaggaatagt tcaaataaaa tctgtagcaa gagaagcagg
    12481 gtcaagaact aaaatggcag taaaatctat agatgaaaaa atagacccaa ttggagcttg
    12541 tgttggacca aaaggactta gagtaaaaaa tatagtggat gaacttggcg atgagaaaat
    12601 agatataata aaatatagtg aagacccagc agagtttata tcagcagcac ttagcccatc
    12661 aaaagttgtt agagtagata tagatgaaga tgaaaagtct gccttagtaa tcgttccaga
    12721 ttatcagtta tcgcttgcta taggtaaaga aggtcaaaat gcaagactag cagcaaaatt
    12781 gactaactgg aaaatagata ttaagagcga atctcaagca gaacaagaaa tgttaaatct
    12841 tgaaaattcc aaagaaatag aagctgagga tgttgaagct gaatttatcg accaagaaga
    12901 gttaggtact tcagaggata attttagtga agaagagtaa ggagggatga ttttgaaaaa
    12961 agtaaaaaaa gtgcctcaaa gaaaatgcat agcatgtcaa gatagagact ctaaaaaaga
    13021 gcttataaga atagttaaaa ataaagaagg aaaaatattt ttagacccaa caggaagagc
    13081 caatggaaga ggagcatatg tatgtaaaag ctctgaatgt ctaaaaaaag ctataaagag
    13141 taaagcatta aataaagctt ttaaaataga tgttccagat gaagtctatg ataatctact
    13201 tatggagtta gaagaatatg aaaaataata aagaaaaagt actttcgttc ttagggctcg
    13261 caactcgtgc agggaaaata gtgtctggtg atgattcaac tttgcttgac cttaaaaaag
    13321 gaaaagtaaa tttgatttta atcgcagaag atgcatcaaa taatactaaa aaacttttta
    13381 aagataaatc aacttttaga aatattccat atctattttt ttcaacaaaa gaggaaatag
    13441 gatttgcaat aggaaaatct ccaagagcag ttgtaggaat aaaagatgaa aatttttcta
    13501 aaaagataat tgaactaata gaaatttaaa ataaaatagg gggtgatctt gtgtcaaaaa
    13561 caagagtata ccaaatagca gaagaattaa atatatcaaa tgaagagctt ataaataagt
    13621 tagctgaatt agatataaat gtaactgaca aagattcagt attagaaggt gaagagttag
    13681 aattagcact tgaaatgtta ggtgaagatt tatctcaaga aaatggaaat gttatagaaa
    13741 tagatggaaa gttgacagta caagtattag caacgaaatt agataaatct ccatcagaaa
    13801 taattatgaa gcttatgaaa atgggaacta tggcaacaat aaatcaagag ataagttttg
    13861 agattgctgc tttggctgcc aaagactatg gatttgaatt aacagtagct gaaagtgatg
    13921 atacagaagc tttagaaata gaagctttaa tggaaattga agaagataaa gaagaggatt
    13981 taaaacctag accaccagtt gttacagtta tgggacatgt tgaccatggt aagacatctt
    14041 tactagatgc aataagaaaa actgatgtga tttcaggtga agctggagga ataactcagc
    14101 atataggtgc ttcagaagtt aaaatcaatg gacacaaaat agtattttta gatactccag
    14161 gacatgaggc tttcacatca atgagagcaa gaggagcaca agttacagat atagcaatat
    14221 tagtagttgc agcagatgat ggtataatgc ctcaaacagt tgaagctata aaccatgcta
    14281 aagcagcagg tgtaccttta atagttgcta ttaataaaat agataaacca ggtgcaaacc
    14341 cagataaggt taaacaggaa ttagcagacc aaggattatt agttgaagac tggggtggag
    14401 aagtaatagc agttccagta tcagctaaga aaaaagaagg aatagataca cttttagaaa
    14461 tggtattatt ggttgctgaa atggaagagt taagagctaa tccaaataaa agagcagtag
    14521 gaactgttat agaagctgag cttgataaag gaagagggcc agttgcaaca gtacttgttc
    14581 aaggtggtac tttaacagta ggtgacccaa tcgttgcagg tgttgcatgt ggtaaagtta
    14641 gagcaatgat aaatgcaaaa ggtaagagag taaaaacagc aggaccatct acagcagttg
    14701 agatattagg tctatcagaa gttccacaag gaggagacca atttgtcgaa gttccaactg
    14761 ataagattgc tagaagtgta gcagcaagaa gacaacaaat agttagagat gaaatgttaa
    14821 aatcaactca aagattatca ctagatgctc tatttagtca aatgagtgaa ggttctataa
    14881 aagaccttaa tatagttata aaagcagatg tacaaggttc agtacaggca gttaaacaat
    14941 ctttagaaaa attatcaaat gaagaagttc aagttaaagt aatacatggt ggtgttggag
    15001 ctgtaacaga atcagacata ttacttgcag cagcttctaa cgcaataata ataggattca
    15061 atgtaagacc agttccaggt gcagaatctc taggagaaaa agaaaatgtt gatataagaa
    15121 catatactat aatatacaag gcaatagaag atatacaagc ggctatgact ggtatgttag
    15181 accctgaata tgttgatgaa gaaacaggaa aagctgaaat aagagaaata tataaaatat
    15241 ctggtgttgg aacagtagct ggatgttatg tgactaatgg taagatattt agaaattgta
    15301 aagtaagatt agttagagat agcataataa tacatgaagg tgagcttgca gcacttaaga
    15361 gatttaaaga tgatgtaaaa gaagttaatt ctggatacga atgtggtatg agttttgtaa
    15421 attataacga cataaaagaa ggcgatatag tagaagctta tataactaaa gaagtagaaa
    15481 gaaaattata aattcgtatc aaagaaaatc agttagtaaa gaaggtcgat ggtatggcat
    15541 catataatag aacaagaagg atagcagaag aaataagaaa agtcgtaagt actatgctta
    15601 taaatggagt aaaagaccct agaataacat ctatggtgag tgtaactgat gtagaagtta
    15661 caaatgacct aagatatgct tatgtatatg taagtattct aggtggagat gaagaatcca
    15721 cactaacagg tcttaaaagt gctggaggat atataagaag agaagtagga aaaaatataa
    15781 aacttagata tattcctgag atagtattta aacttgatga ttcaatagaa aaaggaatgt
    15841 atatggataa ccttataaaa agagttaatg aaaaaaatgc tcaacaaaat gaggatgaaa
    15901 attatgatga ataatatgga tagtattata aactatattt atgagggtga ctattttata
    15961 gttacctctc atatcagtcc agatggagac aatgttggtt ctacactttc aatgtattat
    16021 acattgaaga atttaggaaa aaatgtatat tatgtacttg atgatgaagc tccattaaat
    16081 ttaaggtttt tggttgaagg tgtaaaaatc tataagtcta atgaatttaa tatgaagaac
    16141 tattctttaa ttgcacttga ttgtggggat aagatgagaa tatgtgtgag tgatgaaatc
    16201 aaaaataatg catcaaaatt aatctgtatt gaccatcatg ctagtaatga ttcatatgga
    16261 gatttaaatt atgtagatat tgatgcatct tctacatgtg aactagttta taatttattg
    16321 gttagatttc agcaaacaaa agatattaat attataaatg aagatatagc aacatgttta
    16381 tatacaggtc ttgtaactga tactggaaac ttttcgtatt caaatgtaca tgcaagtagt
    16441 tttgaaatgg caaaaaattt acttgtttta ggtgcacaaa aaaatactat aatacaaaat
    16501 atatatcaaa gcaattcttc tgattattat aaattattag gagaagctct aaaaggtttg
    16561 gagatatttg attcaaaggt atctagtata gttttaactc aagacatgat gaatagaaat
    16621 aatattagct ttaatgatgt tgatggaata acaacatata caagagatat aaaaggtata
    16681 gaagttggaa ttttatttaa agagaaaaaa caaaatgaaa ttaaagtgag ttttaggtca
    16741 aaaaattatg ttgatgtaag tgaaatagcc aagttatttg gcggtggtgg tcatgtaagg
    16801 gctgctggat gtactattag agatagtatt gataatgcaa agaaaatggt actagaggca
    16861 gtgttaaaat caatttaagg tgacagtaaa atgaataaga ttataagtat attgaaaccc
    16921 acaggtatga catctcatga tgttgtaagt agagtgagaa agattttaaa tataaaaaaa
    16981 gttggtcata caggtactct tgacccagat gcttcaggtg ttttaccaat ctgtataggg
    17041 aaagctacaa aagtatgtga agttatttta aacaaagata aatcgtatat atgtgagctt
    17101 actttaggta taagtacaga tacttatgat gcttcaggtg aaattttaaa aaaagttgat
    17161 gattttaaat ttagtaatga agacatagaa agagcttttg atacacaaag aggagagata
    17221 aatcaacttc ctccaattta ttcagcttta aaggtaaatg gaaagagaat gtgtgattta
    17281 gtaagaagtg gtagacaatc tgaaattact ttaaaaacaa gaagagtaaa tataaaagac
    17341 ataaaaatac taagtataaa aggtaataaa gtaatgtttt atgttgagtg ttctaaaggg
    17401 acttatgtga gaagtatttg ccatgatata ggagaatatc tgggttgtgg agctcatatg
    17461 tcgtttttga atagaacatc ttctggtaaa tttgacttag ataattcaat aacattagaa
    17521 gaattagaat tattttatga aaataaaact ttagacaaat acttatatga tatagattat
    17581 gtccttgatt cgtttaatta tgttgtatta aaccctaatg ctataaaata ttatagtaat
    17641 ggtggctcaa tagatgataa aaggttttta aaaaataatt tcgataaaga tgatgaagtt
    17701 gtaagagtat acagtacaga taatttctta ggattaggaa agttatcaaa acacaataat
    17761 acaataagtg ttaaaagtga taagatgttc atataaccaa tgtatttaaa aaaacaacaa
    17821 aagagatagt actataataa cttctatagt ctatctcttt tacttatgct ataatagttt
    17881 gtgaataagt taagagtaaa ttatatttta gcggggaaca tttattatgg tgattataaa
    17941 atctataaaa gaaatagcaa acataaaaga aagtgtcatt accataggaa attttgatgg
    18001 tgtacacaaa ggacatcaag ttcttatagg aaaaacagtt gaatatgcaa aaaaagaaaa
    18061 tatacagagt attgtattta catttgcaaa tcatcctgta aattacttta gaccaaactc
    18121 aacaaaaaat attataagca atgatgataa aataaaatta ttagaagatt tgggaataga
    18181 tatagttgta atcataccat ttgatgagta tatgactaaa atacctgcaa aggatttcgt
    18241 agatgagata ctagtaaaaa aattaaaagc taaaaagata gtaattggac atgactttac
    18301 atttgcaagg gcaaaagaag gcaatgttaa tctattaaaa agcttagaac atgaatttgg
    18361 ttttgaggta gaagtaataa aacccatcaa aatcaatgat gtaagagtga gtagtactta
    18421 tattagaagt ttagtatccc agggtgatat ggctaatgtc aaggaatatt tgggtagaaa
    18481 ttacaagtta gaaggttgtg ttatacattc taaacacctt ggaagaacta taggattccc
    18541 aacggcaaat attgacctta agaataatat gctcgttcca aagagaggta tttatgcttc
    18601 tattgtacat attggagatg agacttattt tggtgctaca aatgtaggct ataacccaac
    18661 agttaatgga aaaactttgt ctattgaaac aaatatttta gagtttgata gagatattta
    18721 tggggaaaat ataattgttg aatttttgga aagaataaga gatgaaagaa agtttaattc
    18781 aatagatgac ttaaaaaatc aattgtataa tgatacaaat tatgtgtatg aaaactatgt
    18841 ttgcaaaaac aagtaatgta tgataaaatg attaaggaaa ataaccatta ctcggcattt
    18901 gaatactcca atcaatgctt agtaataggc gtttctaaaa aaatacggag gtaatctaaa
    18961 tgataaacaa atcagaaata ataaaagaat atggaagagc tgaaggggat actggttctc
    19021 cagaagtaca aatagcttta ttaacagcta gaataaacga gttaaatgga catttaaaaa
    19081 ctcataagaa agaccaccat tcaagaagag gactattaaa aatggttggt agaagaagaa
    19141 acttattagc ttacttaaag gaaaaagatc ttgaaggata tagagcatta atagctaaat
    19201 taggattaag aaagtaattt cattctttat aaaaaagcag gtatagtgta tacctgcttt
    19261 tttatagtta attcattata aagtaaaata gttaataata aaagtagttt atatgttata
    19321 aaaaattata aaatgaataa tattgggtaa gaatattaaa atagtataca aatacataat
    19381 caatttaaaa ggaaagacag gataaatata gaaattttat aacaggataa ttagttggaa
    19441 taaggaggat attattttga aaataaagtt agtatgcgat agtttgtgcg atattccaga
    19501 tgaaatatca gaaaaagatt atatagagat agttccactt acagttattt ttaatgatag
    19561 ggaatatata gaaggagtag atataaagaa agaagagttt tataaaaaag taaaagaaat
    19621 caagcaaata cctaaaactt cacaagctac atatatggag tttaaagaag tgtttgataa
    19681 gttcataaca gaaggttatc atataatctg tatgacaggg gctgcaaacg catcaggaac
    19741 ttttcaaagt gctatgatag ctaaaaatga tgtaaatgaa aatgaaaaaa tacatatatt
    19801 tgatactaga aatttatctc taggtagtgg acaatatgtt ataaaggcat gtgaacttct
    19861 tgaagaagga ctgggatttg aagaaattat agatgaatta gaaaatactc gcagtagtgt
    19921 aagattactt tttgcaccat atacacttga ttttttaaag cagagtggaa gagttccagt
    19981 tgcaactgct ttgataggaa atatgttaaa tataaagcct atatttttct ttgataatgg
    20041 ggaagctaag ttggtaaata aggttagagg aataaagaat atagcaagta aactggtcga
    20101 tataatttta gaaatgaatg aagggtcttt agaaggaaaa atcgtcacaa taggttgtgg
    20161 agataattta cacgattgtg aaatattaaa agatgaagta aataaaagaa taaaagcaag
    20221 aagagtatta tttactcgag gaggagtttc tatttgctct catacagggc cagatatatt
    20281 ggctataagt tgttctaatt agtagtaata taaggttttt gcatatatat gtgaagataa
    20341 ttttatattg ttaatttata gtatttttga taatatagta aatagagtga tatacgtgtt
    20401 ttttaataag aggaggacaa gcattaatgt ttgaacataa aatatttaaa atggattttg
    20461 caggaagaga actttctgta gaaataggaa agatttgtga aatggctagt ggtagttgta
    20521 tagttcgata cagtgacagt atggtaatgg taaatacaac taaatcagcc aaacctagag
    20581 acggaataga cttttttcct ttaagtgtgg attatgaaga aaaattatac tctgtaggta
    20641 agatacctgg aggtttttta aagagagaag ggaagccttc agaaaaagct atacttacat
    20701 caagattgat agatagacca ataagacctt tattcccaaa aggatttaga aatgatgttc
    20761 aggtagttgc aacagttcta tcagtagacc aagattgtac accagatata gtagcaatga
    20821 taggttcttc aatagccctt tctatatcag acataccatt taatggacca acaggttcag
    20881 tatgtgttgg ccttgtagat ggtgcatttg ttgtaaatcc aaacgcagag caaagagaaa
    20941 aaagttcaat gcatctagtt gtttcaggta caaaggaagc aataatgatg gttgaagccg
    21001 gagctgatga agttccagat gaggtaatgc ttgatgctat tttatttgct caccaagaaa
    21061 taaaaaaaat agttgagttt atagaaggta tagttgcaga agttggaaaa gaaaaaatgc
    21121 ctgttgaatt ataccatgca ggagaagaaa taactcaatt ggttcgcgaa tttgcaactg
    21181 ataagatgaa aaaagcagtt cagacttttg aaaagttaga gagaatggaa aatatggata
    21241 gagttaaaga agaaacttta gctcactttg aagaaacttt agaagatttt gaagattttg
    21301 ttggagatat agaagaagta ttacaagata taataaaaga agaagtaaga aaacttatag
    21361 tacatgaaaa tgtaagacca gataatagaa aacttgaaga aataagacct atatggtgtg
    21421 aaactggcat gatacctaga gctcatggtt cagccatatt tactagagga caaactcaag
    21481 tacttaatgt tgctacttta ggtgctttag gagatgttca aaaattagat gggttagatg
    21541 aagaagaaaa caagagatat atgcatcatt ataatttccc cgcatatagt gttggagaag
    21601 ctagaccatc aagaggacca ggaagaagag aaataggaca tggtgcttta gcagaaagag
    21661 ctttattacc agtaatacca tctcaagaag aatttcctta tgctattaga ttagtttctg
    21721 aagtattaag ttcaaatggt tctacatctc aagcaagtgt atgtggctca acattatctt
    21781 tacttgatgc aggtgtacct ataaaagata tggttgcagg tatagcaatg ggacttataa
    21841 agcatgatgg taaggttgca gtgctttcag atatacaagg tatggaagac catttaggcg
    21901 atatggactt taaagtagct ggaacagagt atggtataac agcaatacaa atggatataa
    21961 aaattgatgg tatagataaa gaaattctac aacgtgcttt aaaacaagct aaagagggta
    22021 gaatccatat actaggagag atgagaaaaa ctatatctca gccaaaacca gaactttcac
    22081 catatgcacc taagattgtt aaaatgcaaa taaatcctga caagataaaa gatgtaatag
    22141 ggcctggtgg aaagataata actaagataa tagacgaaac tggagttaag atagatattg
    22201 aacaaactgg ggaagtattt atatcaggta ttgagattga tatgattaag aaagctcaag
    22261 aacttataaa taatatagta gttgaaccag aagtaggaaa aacttataaa ggtaaggtaa
    22321 gtagaataat gaactttgga gcatttgttg aaatacttcc aggaaaagaa ggattacttc
    22381 atatatctca tatagctcat gaaagagttg ccaaagttga agatgtattg aatatagggg
    22441 atgaagttga agttaaagtt acagagattg atgaaaaagg aagagttaat ttatcaagaa
    22501 aagttctttt accaaaacca gaacataaaa ataaataact tattatataa ttaaaagcaa
    22561 agtcttagga ttttgctttt ttaaatacaa aaaagtagat aattttaaaa tgatagttat
    22621 ggatggctta tagttctttt tctgtaaggc tttactctta taaagaagct atttaaaagc
    22681 tgattaatag aaagctttat tgatatatat agaatcactt aagttatatt tagtgcataa
    22741 acagagctta tttttaatag tattaaataa gaaacaattt gtacataatt agggggagtt
    22801 ttaatgataa tgatggtact taataagagt aagctcaaaa aaatcataat actaatttta
    22861 gtagtgttat tagtattatc aggaggggta atggtattaa agttatttaa caacaagcca
    22921 gtatttaatt tatttaatgg tgtagattta ccatatgaaa gaggtacaaa ggataaaagt
    22981 ggatatgttg cattaacctg taatatagac cttggatggg aaacagagta cgtagaatcc
    23041 attttagaaa cacttaaaaa agaaaatgta aagattacat ttaatgtaac tggaaaatgg
    23101 gcagaaaaga ataaggatga attattaaag ataaaaaaac aaggacatga aataggtaat
    23161 catggatata aacatttaga ttattctaca ttatcatatg aagacaatta tgagcaaata
    23221 gagacctcta aaaagataat tgaagaaatt ataggtgaaa aaactaaatt ttttcaagca
    23281 ccagcaggtt cctttggtcc agaaacagta aaggctgcta aagcattagg gtatacttct
    23341 atcaaatggg atgccgatac tatagattgg aaatacaaag accaaccaga ggtaatcatt
    23401 gatagaatga agaaaaaaga cattaaagat agtagtataa tcttaatgca tccaaccaac
    23461 gcaacgacaa aatgtataga tgatattata gctattgtaa gagaaaaagg actaaaacca
    23521 gggaaactta gtgatgtatt taagtagaca aagtataaat atttggttaa gattgtataa
    23581 gttgtagttg aattaattaa aagactgact tataatgtaa tacatataaa aaataaatat
    23641 aattaaaaca tagtgttttc aataatccaa tatgaaacaa agaataaatt gttaggagaa
    23701 tgtaatgtat aagacaaaaa tactggaaaa tgggcttaca ataattggtg aagaaatacc
    23761 atatctaaag tcaataacat taggtatttg gataaatgca ggctctagga tagaagaagc
    23821 acaggtgagt ggaacttctc attttataga gcatatgatg tttaaaggaa ccaaaaacag
    23881 aacatcaaaa gagattgcta gttcaataga taatttgggt ggtcaaataa atgcatttac
    23941 aagtaaagaa tgtacatgtt attatgtaaa actaatagat gagcatatag atacgggaat
    24001 tgatgtattg agtgatatga tactaaattc taaatttgat aaaaatgata tagataagga
    24061 aagacttata atattggaag aacttaagat gtatgaagac tctccagatg acttatctta
    24121 tgatttatta gtggaaaata tatatgctaa tgatggatta ggaatgaata taattgggac
    24181 aaaagaatct ctatacaata taactagaga atccatgtta gaatacttaa ataagtatta
    24241 tattccaaat aacgctgtta tttctatagc tggaaacttt aactttgatg atatggtgga
    24301 gaaaatcaaa tcaaaatttg gacactggga aaagaaaaat ttgagtatag acataagtga
    24361 ggctaagttt aatccttgct ttatatcaaa aaataaagat actgaacagg tgaacttagc
    24421 tatctgccta aaaggtattc cttttgaaaa tgatgaagaa gtctactcaa tggcagtggt
    24481 aaataatatt ttcggtggaa gtataagctc aaggcttttt cagaaaataa gggaagaaaa
    24541 aggtttagta tattcaatat actcatcaca gacattatat aggaaatgtg gtgaattagg
    24601 catatttgca agtatgagta cagaaaattt acaagatgta tataatttaa taaagaaaga
    24661 aattgaaaat ataagagaaa actatcttac agaaaaagaa atatctgaaa gcaaggaaca
    24721 gttaaaagga aattatatat tggatttaga aagtactagc agtagaatga tgtctactgg
    24781 taaatctatg ttacttagca aaaaagtaaa aactacagat gaaatacttg aatgtataaa
    24841 taatgttaat attaattcaa taaagaaggt tgttgataag gtattcaata tcgaaaatat
    24901 tggtacatgt atagttggaa gagatgtaga aaagattcta cacttggatt aatattgaaa
    24961 aaagtaaatc tctggaggta taaaatatga atttatctga aatagcaggt aaggaaatag
    25021 ttaacttggt cacaggagaa agattggggg tcattggaga atgtgacctt atactagatg
    25081 aaactacagg cggtatatta gcgcttctaa ttccaaagga aaaaggattt tttgccagaa
    25141 gaaaagataa gtcattttta gaagtaccat ggagaaatgt aaaaaaaata ggtaatgaca
    25201 tgataattat agaatatgag ggatatattt aggaggaaaa aatgagtatt ttagtgcaaa
    25261 agtttggtgg gacatcagta gaatcatatg aaaaaatgaa tgaagtatgt aagatagtaa
    25321 aagcatacaa aaaaaatgat gaagaattac aattagtatt agtagtttca gcaatgggaa
    25381 gaaaaggagc tccatatgct acagatacac ttataaattt gtgtagtgca gtaaatgatg
    25441 agccaagtaa aagagaactt gatttaataa tgtcttgtgg agaaataata tcaggaacaa
    25501 ttcttgctaa tttattgaat gcacagggga tagattctgt tttctttact ggtcagcaag
    25561 caggtataat tacaagtgat gaatattcta atgcaaagat aaagtatata aatcctaaaa
    25621 aggtaaaaag agctttagat gatggtaagg ttgtagttat agctggattc cagggtgtaa
    25681 cagatgatgg agaaataact actcttggaa gaggaggaag cgatacatca gcagttgcta
    25741 ttggtaaagc tttagaatgt gaaacagttg aaatttacac tgatgtggat ggtataatga
    25801 ctgcagaccc tagagtagaa ccaaacgcta aagtattgag ttttatagat tatgaggaag
    25861 tattccaaat ggctgacaaa ggtgcaaaag taatacatcc aagagcagta cagttggcta
    25921 aaagtggaaa tataacttta gccataaaga atacaatgaa tccaactttt gaaggtacaa
    25981 aaataggttc tttatgtaga cacttagaag agaatataga atatgaacaa gaaagagatt
    26041 ttaaggttgc tgttgcaaat aaagatagtg tagcacaagt taagattaaa tcagcagaag
    26101 aagtgtttac agaagtatta aatgagatag aaaagaaact tataactata gatatgataa
    26161 atttctttat atcagaaaaa gcatttgttg tagaagatgc tgatataaaa tcattaaagg
    26221 aaatactaga taaatttgaa ttagattatg aagtaaatag agactgtgct aaggtaacat
    26281 tgatatgttc aagaattgct gatgaaatgt ctggtatcat gtcaaaggta gttagaggat
    26341 tatcaaaagc aggggtatct cttttacaaa catcagactc aaatatgact atatcttgtt
    26401 tagttagtga agaagatatg catacagcag tacatgctat acaccaacaa ttttatttaa
    26461 aataaactgt ataatacaat tattttttaa tcaaatttaa gaatttcata tatatcttaa
    26521 tatatttgat ttatttggta aaactaaatt aaatataatt ttgataggag tttttttatg
    26581 aaagattata gagaagataa tgaaagattt gataaagata attgtagtga agaatctagt
    26641 tgcgaagaga gagaatgtat aaagcaattt ggtaccaatg aaatgccacc tcaacctcct
    26701 aaagatattc aatgtataac tataattgga gaaattgagg gacattttat aggaaatcct
    26761 caaaaaaaag ctacaaagta cgagcatata attccaatgc tatactcaat tgaggaaagc
    26821 aatgatgtta aaggagtgtt agttgtttta aacactgttg gaggagatat tgaagcaggt
    26881 ttagctattg cagaactttt aaacagtaca tctaaaaaag tagttactct tgttctagga
    26941 ggaagtcata gtataggtgt tcctcttgct acagcaggag attactcatt tatagcacca
    27001 acagcaacca tgattataca tccagtgcga actactggtc ttgtgattgg tataaatgag
    27061 acttttgaat attttaaaaa gatgcaagat agaataattc aatttataat aagaacatca
    27121 aatatcaaaa aagatgtact tgaaaaactt atgcatgaaa aagatgaact tgtaagtgat
    27181 gttggtagtg tattaatagg aaaagaagct gtggattatg gtcttataga tgaagttgga
    27241 ggactaaaag aagcacttaa aaaattaaga gagcttataa aagaatctga ggaagaaaaa
    27301 aataatgatt aaaatttgtt taaataataa aatttatgat ataatgatgt aataaagtag
    27361 gagtgttagt gttaaggcat tagcactttt tgctgtgaag gggtaaaact tgctaaaaat
    27421 aaggagtgat attaatggcc acgaaaaaga agaaaaaaaa gaaggtaaat accttaagtt
    27481 ttaatgcaga atatcataat ttaataacaa tatttatagg tgtattttta ttatatagtt
    27541 taaattctaa ttctatggga tggatacctg tgttaatgca aaatcttttt aagggactat
    27601 ttggagggct ttcaatagca ataccattta tagtaattat aacaggactt ttaggatttt
    27661 ttgatggaaa tgaatatata tataggctaa gaaagactaa actatactac ataattattt
    27721 tatttatatt tgtattttat ggattattaa atgcaggaac tcttcctgta gatagcccac
    27781 taaaaggtaa catgtttgat gatgttatga aattgggtgt aagtggacaa ggtagtggtt
    27841 tgatagcaac aactattgcg tattatatga gtaaaatatt tggtatagca ggtggatggt
    27901 taataagtat cttcgcactt atactttctg taatgtttat atttaatatt tctataaaag
    27961 atttggtgtc aaatgcaaaa tcaaaatcta aagcatctaa agacagcaat ttaacgttta
    28021 aagataaaat tgcaaatatg aaaaaatctg caatagatat gatgactgat gaagtagatg
    28081 atactactat aaataaaaaa ccaggtttct ttaaagggtt aatgtcaaaa ggtcttaata
    28141 atgatgatga agaagatgaa tattttgaag aagaaaatag tgatggtgta gatgataaaa
    28201 ctataaaaat agttggattt aataaggcag aagatgagta tttagaaata ttagaaggaa
    28261 ctcaaagtat gccagagtta gatgttttaa aagaattaca aaaagctacc aatgaaaatc
    28321 cagttgtaga tactaaacca gaaaaaaaag ttgatatagc taaatctaat ttaaatatag
    28381 aaaaaactca acctatgagt attgtagcag agccagttaa tgaggattat tcaaactata
    28441 aaaagcctag cattgagctt ttaaataaag taaataagaa gtctgatgaa aatggtaaaa
    28501 agaaagtgtt gaaaaatgct tctcttttag aaaagacact atcagatttt ggagtggaag
    28561 caaaaattaa tcaagttaca gttggaccta ctataacaag atatgaaata caaccaagtc
    28621 caggagtcaa ggtaagtaaa attgtaaatt taactgatga tatagcttta agtttagcag
    28681 ctaaaagtat aagaattgaa gccccaattc ctggtaaaag tgcaattgga atagaagttc
    28741 caaatgaaga agctcaaatg gttggtgtga gagaagttct agaaagtgaa gaatttaata
    28801 attttaactc tcctcttgca atgggacttg gaaaagacgt agcaggaaaa attataattg
    28861 gagatatagg taagatgcca cacttactaa tagctggttc tacaggttca ggaaagagtg
    28921 tgtgtgtaaa tacattaata agtagtattt tatataaggc aaatcctgat gaagtaaaat
    28981 tactattaat agacccaaaa gtagttgaac ttgcaaatta taatggaata ccacatctat
    29041 tgataccagt agttactgac cctaaaaaag ctgcaaatgc tttaaattgg gcagttacag
    29101 agatgaatag aaggtataaa ttatttgcag atgctcaagt aaaagatgta acaagttata
    29161 atgagaaagc ggaagaaaaa cttcctaaaa tagtaataat aatagatgag ttagcagatt
    29221 taatgatggc aagtgcaaat gatgtagaag attatatttg tagattagct caaatggcaa
    29281 gagctgctgg aatgcacttg attgtagcaa ctcaaagacc atctgttgac gttataacag
    29341 gggtaataaa ggctaatata ccatctagaa tagcatttgc agtatcatct caaactgatt
    29401 caagaacaat ccttgatatg ggtggtgcag agaagcttct aggtaaaggt gatatgttat
    29461 tctatccact tggtgcagca aaaccagtta gacttcaagg ggcattcata tcagagagtg
    29521 agtcggaaaa agtaattgat tttgtaaaaa gtcaggttaa agatggaata aaatatgaag
    29581 aagatataat tgaaactata tctaaggtta atacttcaaa gagtagtgat gaggatgagt
    29641 ttttgagtga agcgatagaa tttgtcgtgg aaagtggtca agcatctgcc tctatgctac
    29701 agagaagatt taaaatagga ttcaatagag cagctagact tatagattct atggaagaaa
    29761 gaggcataat tggagcaagt gagggaagta aacctagaaa ggtattaata agcaaacagg
    29821 atttgcagaa tttagaaggt gaatagaatt gtcagttata aaaatagaaa aagcacttaa
    29881 gctagataaa gtaatatttg tagatgtgag aactgaagga gaatatgaag aagaccatat
    29941 attgaatgca atcaatatgc ctctttttaa aaataatgaa cataatgaag ttggaacaat
    30001 ttataagatg cagggaaaac atgaagcaat acaaaaaggg tttgactatg tatcgtataa
    30061 attaaaggat atatatttac aagcagcaga acttgcatta aattatgata atattgtcat
    30121 atattgtgca agaggcggaa tgagaagtgg aagtatagta aatttattat catcattagg
    30181 tgtaaatgta tatcaacttg aaggtggata taaggcttat agaaattttg tattagagta
    30241 tctaaggcat gtaatggata caaaaaactt tgtatctgtt catggattga ctggtacagg
    30301 aaagactgat ttgttacatt tattagatga aaaaggaata gatgtactgg atttagaagg
    30361 catagctaaa aacagtggtt ctacctttgg ttttataact tttgataaaa agccaccatc
    30421 tcaaaagaac tttgaaacaa aactatttga aagtgtattt ttttcgaaag agaattatat
    30481 atttgtcgag agtgaaagca aacgagttgg aagtgttaca gttccaaatg aaattttcga
    30541 ggcaatgaca cgagaaggac atcatatatt attagaatgt gatattgaaa atagagttga
    30601 tagactttgt agagattata tttatgataa agatgaaggt aatatattga ttttaaaaga
    30661 atgtataaac aagtttagaa aaagattggg taataaagta gtagatgatt atattgattt
    30721 attagaatca ggtgaatata aagaacttgt aaaaagatat cttttagaat actacgaccc
    30781 actttatatg cattcagtag agaaatataa gtatagtaag attataaatt ttaatgatac
    30841 acaaaaagct ttagaagatg ttatagactt atatgagagt atattggaag gagaaagtga
    30901 atgttaaaaa tagcattgga atcattagga tgctcaaaaa atttagtaga tgcagaaata
    30961 atgatgggaa ttttaaataa caaaggctat aaattaattg gagattttga agaagctgat
    31021 gtaattatag taaatacatg tgggtttata gagtcagcta aacaagaatc tatagatacg
    31081 ataataaact tttcagagtt aaaaaaaact ggcaatttaa agttgttgat agtaacaggg
    31141 tgtctagccc aaagatattc tgaagaatta aaaactgaaa ttcctgaaat agatgctata
    31201 gtaggaacag gaagctatca aaatatagat aaaatactta aagaattaag tgaaatgcat
    31261 caaattgtaa gccttaatga tatagagttt gtatttaatg aagatttacc aagatatata
    31321 tctacaccta gctatatggc ctatttaaaa attggagaag gatgctccaa taattgtact
    31381 tattgtataa taccaaaact aagaggtaaa tatagaagta gaaaatttga agatataatt
    31441 aaagaagcta agaaattagc tgaatctgga gtaaaagaac ttgtggttat agcacaagat
    31501 acgacaaagt atggttttga tttatatgga aaagaaagat tatcagaact tttagaagaa
    31561 cttgcaaaaa tagatggatt taagtggata agggttatgt attcatatcc agaatctata
    31621 acagaggaat tgatacaagt tataaagaaa tatgataata tatgtagtta ctttgatatg
    31681 ccaatacagc atgcaagtaa caatatttta aaattaatga atagaaaaac tactaaagaa
    31741 gatatattaa ataaaatcaa tctgataaga agtaatatac cagatgctat acttagaact
    31801 acgataatag tagggtttcc tggtgaaaca gaagatgact tcaaacaact tgtggacttt
    31861 gttgaagagg ttaaatttga tagacttggt gcatttgcat attcaagaga agaagataca
    31921 ccagcagata gattgccaaa ccatatagat gaagaagtaa aaatacaaag aagagatact
    31981 ttaatgatga ttcaacaaaa aatatctgaa gaacttaatg acaaaaagat aggaaaaact
    32041 tatgaggtgt taatagaaga acagatagaa gataatgtat atacaggaag aacacaaggt
    32101 gatgcggaag aaattgacag tatagtgtat gtaaaaagtg tagacaatct tgaagtagga
    32161 gaatttgtaa gtgttcaaat aaatgatgca atggaatacg atttaatggg agatgtttta
    32221 tatgaacttg cctaacaagt taactttgtt tagaatattt ttaataccag tatttgtttt
    32281 aattatgttg ctcaatgtac caaataaatt tttaatagct tgtattattt ttataatagc
    32341 ttcaattact gatgctttag atggaaaaat agctagaaaa tacaatctag tcactgactt
    32401 tggaaaattt atggacccat tagcagataa gttattggtt atttcagcac ttacatgtat
    32461 gatagaagac catcttgtaa gtagttggat ggtaattata atagtggcta gagagcttac
    32521 agttagcata ttaagagcta tagctgctgc tgatggaaaa gtaattgctg ctggaaacag
    32581 tggaaaactt aaaacgataa ctcaaatggt atctatagtg tttttattgt taggcgctca
    32641 gtttgaaaat gtattgatat taaatattgg tgaaattctc attcttatag ctacactact
    32701 tacattatat tctggatggg aatatctata taaaaataaa gagcttttta tgtcttctaa
    32761 gtaaaaaatt atgctttaat aaataaaaac taatgattaa taaataaaaa ataacaactt
    32821 aaaattttaa aaatgtaaaa aagggtatac tattatagta tacctctttt cattttaaga
    32881 atattttctt ttgtgtatat aattattaat aatggtttat aaataaaatg taatgaataa
    32941 aaatataatc attagtagcg tttttaaatt gactaggttt gatttatgtt atataattaa
    33001 tatataacaa gagaacaaat gtttgtagat aaatgaaggg atggtataga gatgagtgta
    33061 gatcaagaaa aattaaaagc gttgaatgaa gctttaggta aaattgaaaa agattttggt
    33121 aaaggttcag taatgaaatt gggagaagca acatctatgt ctatagatgt tatatcaaca
    33181 ggagcgattg gtttagacat agctattggt ataggaggtc tacctagagg gagaatagtt
    33241 gaagtatatg gtccagaatc ttctggtaag actactgttg cgcttagttg tgtagcatca
    33301 gctcaaaaag atggaggaat agctgcattt atagatgcag aacatgcact tgacccagta
    33361 tatgcaaaag ctttgggtgt ggatgttgat aacctaataa tatctcaacc agatacaggt
    33421 gaacaggctt tagagatagc agaggcattg ataagaagtg gagcgataga tataatagta
    33481 atagactcag tagcagcatt agttccaaag gctgaaatag atggagatat gggtgattct
    33541 catgtaggtt tacaagctag acttatgtca caagcactta gaaagttaac tggttcaatt
    33601 aaaaaatcaa attgtgttgc tatatttata aaccagttaa gagagaaagt aggaataatg
    33661 tttggaaacc cagaaactac tactggagga cgtgcactaa aattctattc atcagttaga
    33721 ttggatgtta gaaaaataga tacaataaaa caaggtgata aagttatagg tagtagaact
    33781 agagttaaag ttgttaaaaa caaagtagca ccaccattta agcaagctga atttgatata
    33841 atgtatggag aagggatttc aaaaattgga gaccttttag atatagctgc tgatgtagat
    33901 atagttaaaa aatcaggttc atggtatagt tacaatgata ctaaacttgg acaaggaaga
    33961 gaaaatgtta aaaaattctt ggaagataat ttagatttaa ctactgaaat agatgagaaa
    34021 gttagagcat tttacaattt aaatgaagaa catgaagaat caggtacttc agtatcaaaa
    34081 gaaattgtag aagaataagt aaaaatacaa aaaaataact agtaattaaa gacatgccac
    34141 gctccctatt agcttgacac aatgttgaaa aaattataaa attaagtgag gataaaatat
    34201 ctaaatttaa ttttaattat taggaatatt ttttaatcat agtgatgaca ttttgggatt
    34261 attaacgaag gaggtggatg tcatagatag tgtagtaatg attgtaatag gtgcagctgt
    34321 tggtattata gctggatatt ttgttagaaa aaatatatct gaagcaaaaa ttggacaagc
    34381 tgaaaacttg gccaaagaaa taatagataa agcacaccac gactcagaaa cagtacaaaa
    34441 agaaaaatta ctagaagcta aagaagagat ccataagtgg agaacagaag ctgaaagaga
    34501 aaacagagaa agaagaacag aagtacaaaa gtatgaaaaa agagtagtac aaaaagaaga
    34561 agtcttagat agaaaattac aaaatttaga atctaaagaa gtaaacctaa gtgaaaaatt
    34621 aaaggtagtg gaaaagaaag aagaagaagt cgaagtaata aaaactcaac agttagaaaa
    34681 attagaaagt atatcaggaa ttacttctga taaggccaaa gaaatcatct taaccaatgc
    34741 ggagagagat gtaagacgtg aaatgtctat aatgataaaa gagatagaaa gtcaagctaa
    34801 agaagaagct gataagaaat caagagaaat tataggatat gcaattcaaa aatgtgcagc
    34861 agatcacgta gccgaaacta ctgtaacagt agtaagcttg cctaatgatg aaatgaaagg
    34921 tagaataata ggtagggaag gtagaaatat aaggacttta gaaaccttaa caggtataga
    34981 ccttataata gatgacactc cagaagccgt aattttatca ggatttgatc caataagaag
    35041 agaaattgca agaattgctc tagaaaaatt aatagcagat ggaagaatac atccagctag
    35101 aattgaagaa atggttgaaa aggcgagaaa agaagttgac aatattataa aagaatatgg
    35161 tgaacaagct gcatttgaaa ctagtgtaca tgggattcat ccggaattaa taagattatt
    35221 aggtagactt aactatagaa ctagttatgg acaaaatgta cttaaacatt ctatagaagt
    35281 tgctcatata gctggtataa tggctgctga aataggagca gatattagat tggctaagag
    35341 ggcaggttta ttacatgata taggtaaggc tgtagaccat gaaatggaag gtacacatgt
    35401 tgaaataggt atggatttac ttagacgtta taaagagtct aaagaagtta tacatgccat
    35461 gagtactcat catggagatt acgaacctca aacagtagag gctgtattag ttacagcagc
    35521 agatgcaata tcagctgcta gacctggtgc gagaagagaa actttagaag catatataaa
    35581 gagacttgaa aaattagaag aaatagctaa ttcatatgaa ggtgtagaaa aatcatttgc
    35641 tatacaagct ggtagagaaa taagaataat ggtaaaacca gaagctataa atgatgaaga
    35701 aatacatcta ttggctagag atatgactaa gaaaatagag gatgaacttg aatatcctgg
    35761 acaaataaaa gtaagcataa taagagaaac aagagctata gagtatgcga agtaaaagaa
    35821 ataatatcta aataataaga aaatattttt agaaatttaa atttgccaca agatatgata
    35881 atatatcttg tggcttttaa attgtttaag tttaatttta tataatgcaa aaataatcta
    35941 agtgctaaaa gtgctactta caaaagatta ataaaataaa gctttatata tgtattattt
    36001 atataattat ccaagttt
//
LOCUS       DAELSY010000024        35744 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.024, whole genome shotgun sequence.
ACCESSION   DAELSY010000024 DAELSY010000000
VERSION     DAELSY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.024"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(147..659)
                     /locus_tag="KPG09_001068"
     CDS             complement(147..659)
                     /locus_tag="KPG09_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0965691.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKELSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVKQILDLPVKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            1109..1585
                     /gene="purE"
                     /locus_tag="KPG09_001069"
     CDS             1109..1585
                     /gene="purE"
                     /locus_tag="KPG09_001069"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBG0965692.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            1591..2289
                     /locus_tag="KPG09_001070"
     CDS             1591..2289
                     /locus_tag="KPG09_001070"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG0965693.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            2301..3668
                     /gene="purF"
                     /locus_tag="KPG09_001071"
     CDS             2301..3668
                     /gene="purF"
                     /locus_tag="KPG09_001071"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBG0965694.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            3662..4738
                     /locus_tag="KPG09_001072"
     CDS             3662..4738
                     /locus_tag="KPG09_001072"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBG0965695.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            4732..5325
                     /locus_tag="KPG09_001073"
     CDS             4732..5325
                     /locus_tag="KPG09_001073"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBG0965696.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            5318..6850
                     /gene="purH"
                     /locus_tag="KPG09_001074"
     CDS             5318..6850
                     /gene="purH"
                     /locus_tag="KPG09_001074"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBG0965697.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEVLKQKKNLRILKLAKIEDSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKAKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            6861..8111
                     /gene="purD"
                     /locus_tag="KPG09_001075"
     CDS             6861..8111
                     /gene="purD"
                     /locus_tag="KPG09_001075"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBG0965698.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKVYCAPGNAGITNVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMETILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMLDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            8136..11942
                     /locus_tag="KPG09_001076"
     CDS             8136..11942
                     /locus_tag="KPG09_001076"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBG0965699.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLRTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAVPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKTFTDDKLFE
                     ASYGNIVLELANDDLCMLEGYNYVVLGSTTKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERVGA
                     NANIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVFKNIIGEKDQKIFES
                     GVKYFR"
     gene            12148..13020
                     /gene="rfbD"
                     /locus_tag="KPG09_001077"
     CDS             12148..13020
                     /gene="rfbD"
                     /locus_tag="KPG09_001077"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBG0965700.1"
                     /translation="MKILITGSNGQLGKELVSQLEAINQSINQPKYVILATTRNDLDI
                     SNQTNIDNFILHNKPDVIVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LVHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFELKNIDIKVNPIKSNEFKCKAQRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            13129..14010
                     /gene="rfbA"
                     /locus_tag="KPG09_001078"
     CDS             13129..14010
                     /gene="rfbA"
                     /locus_tag="KPG09_001078"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBG0965701.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLKITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDMQYGR"
     gene            14047..14604
                     /gene="rfbC"
                     /locus_tag="KPG09_001079"
     CDS             14047..14604
                     /gene="rfbC"
                     /locus_tag="KPG09_001079"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBG0965702.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFCDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVRYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFNYKCTNYYYPEFEHGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICIRR"
     gene            14642..15625
                     /gene="rfbB"
                     /locus_tag="KPG09_001080"
     CDS             14642..15625
                     /gene="rfbB"
                     /locus_tag="KPG09_001080"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBG0965703.1"
                     /translation="MKKILITGGAGFIGSNFVHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDSSNYKFVKGDISNREQMYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSPINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLDKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            15664..16332
                     /locus_tag="KPG09_001081"
     CDS             15664..16332
                     /locus_tag="KPG09_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG0965704.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNSSSGFDMIMMTLLK
                     ALSQNNQQTSNGYNLSSNSENLFDELGSVVNNTASRFIDVDTKDKSVKSRIENAIEQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            16341..16769
                     /locus_tag="KPG09_001082"
     CDS             16341..16769
                     /locus_tag="KPG09_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBG0965705.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            16793..17107
                     /locus_tag="KPG09_001083"
     CDS             16793..17107
                     /locus_tag="KPG09_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG0965706.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     VN"
     gene            17259..17534
                     /gene="flgM"
                     /locus_tag="KPG09_001084"
     CDS             17259..17534
                     /gene="flgM"
                     /locus_tag="KPG09_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBG0965707.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGVKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            17539..17955
                     /locus_tag="KPG09_001085"
     CDS             17539..17955
                     /locus_tag="KPG09_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBG0965708.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDRYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTATYNSYGNIRK"
     gene            17973..19283
                     /gene="flgK"
                     /locus_tag="KPG09_001086"
     CDS             17973..19283
                     /gene="flgK"
                     /locus_tag="KPG09_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBG0965709.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGNTVSKYDYY
                     NTIETIINEPSDYGISAGIDDFFKGWESLAKDINSDSKKSLVVENASNLATLISESYK
                     KLNKLKGDIDDNINSEIKQINDMLKSLEDLNKSIDVISGSGSTPNDLLDERDRILDDL
                     SFKLDLENSDVKNMLSDGKLELNELKNADGTWKTGVSGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKVNPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESVGLDSQKVNQDEVNQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            19302..20234
                     /gene="flgL"
                     /locus_tag="KPG09_001087"
     CDS             19302..20234
                     /gene="flgL"
                     /locus_tag="KPG09_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBG0965710.1"
                     /translation="MRVSTGMMSSNYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            20250..20642
                     /locus_tag="KPG09_001088"
     CDS             20250..20642
                     /locus_tag="KPG09_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBG0965711.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDSLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            20636..20848
                     /gene="csrA"
                     /locus_tag="KPG09_001089"
     CDS             20636..20848
                     /gene="csrA"
                     /locus_tag="KPG09_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBG0965712.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            20870..21265
                     /gene="fliS"
                     /locus_tag="KPG09_001090"
     CDS             20870..21265
                     /gene="fliS"
                     /locus_tag="KPG09_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG0965713.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            21265..21621
                     /gene="fliS"
                     /locus_tag="KPG09_001091"
     CDS             21265..21621
                     /gene="fliS"
                     /locus_tag="KPG09_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG0965714.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELIRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            21642..23165
                     /gene="fliD"
                     /locus_tag="KPG09_001092"
     CDS             21642..23165
                     /gene="fliD"
                     /locus_tag="KPG09_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBG0965715.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLKVIGNDNLAQQIANDNGIT
                     FANDASGNKVASVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            23168..23503
                     /gene="fliT"
                     /locus_tag="KPG09_001093"
     CDS             23168..23503
                     /gene="fliT"
                     /locus_tag="KPG09_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBG0965716.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISSKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            23605..24477
                     /locus_tag="KPG09_001094"
     CDS             23605..24477
                     /locus_tag="KPG09_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HBG0965717.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTTN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTDAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            24570..26711
                     /locus_tag="KPG09_001095"
     CDS             24570..26711
                     /locus_tag="KPG09_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0965718.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRSSIESELIIVDTGSTDD
                     TINISRKYTENVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNIDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFEELKENDNSPYTNYQLGKNFIILGKYHEAL
                     YYLEKSYELYSKLESAPGYLITSLVKTYLYLGKHKKCEKLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYESVISEYNNIEDMEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YKNIEEVINNIKDDEKEHVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKVAFEDKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDI
                     KKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYRNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNDANEKLEYIHSMRKLLNEYPFYNKIIKILISELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKSLVDDYSKTFMDDVKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            26735..28612
                     /locus_tag="KPG09_001096"
     CDS             26735..28612
                     /locus_tag="KPG09_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0965719.1"
                     /translation="MNTPIVVNEKSKDIIKDWQYTYTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFMFAHKINTSNINLWKYRLKLVGFCMLIKKSVLDNVGVLDELFTPGNFEDDDLSFR
                     MLESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIADIEKVKLDYEKDFFDYIILGDVLEHLTDPWKIVNELKQYLKKDGCLISSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSVTATSMPLSEID
                     NRFIDTMCKESCKEMRQQYESYQYIVKATKSIDTKRYSSISMVNLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLVDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSKKVQSESDIQDILYFIEGKRY
                     E"
     gene            28605..30719
                     /locus_tag="KPG09_001097"
     CDS             28605..30719
                     /locus_tag="KPG09_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG0965720.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDAFIINKNFIYDILEIFKKNTDVGMIGVAGVKDI
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVANINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLDIVSKIAHFS
                     VFNHTGLYSCEKCERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAIDDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     ASQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKEKQSEIKHFVIDKHCIEIKNYIDKIYKEANHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            30728..31723
                     /locus_tag="KPG09_001098"
     CDS             30728..31723
                     /locus_tag="KPG09_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HBG0965721.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIIYKGEEILAVVPACETYENGERVFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLETYLQEQSFNKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKK
                     DILANFRYSKRRYFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            31815..33047
                     /locus_tag="KPG09_001099"
     CDS             31815..33047
                     /locus_tag="KPG09_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBG0965722.1"
                     /translation="MKRLMILGSIKYFENIVMSAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAIMKKYFKNNAIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLLYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEIFDENNENMIENF"
     gene            33067..34011
                     /locus_tag="KPG09_001100"
     CDS             33067..34011
                     /locus_tag="KPG09_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HBG0965723.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSDIFLYNMKDAKLKAILDGNYITA
                     FRTAAVAGLAIDTFAKKDFNTISIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFIKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYNVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLNCEEIELKEPKEKYWI"
     gene            34105..34512
                     /locus_tag="KPG09_001101"
     CDS             34105..34512
                     /locus_tag="KPG09_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HBG0965724.1"
                     /translation="MDKYNLIKFLSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKESIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNM"
     gene            34599..35690
                     /locus_tag="KPG09_001102"
     CDS             34599..35690
                     /locus_tag="KPG09_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBG0965725.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVRHFEEE
                     FASYVGSKYCVGLASGLDALIIAFRALGISEGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASKMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKNGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFVGMEDEEVSYIIKIINEY"
BASE COUNT        14617 a         3591 c         6546 g        10990 t
ORIGIN      
        1 aatattcaat tggtatataa ataataaatg atagttaaga tttaccttag aggctaatta
       61 ggtgttcagt caaaattaat gcctttagaa tacaaatgta ttctaaaggc atttttacta
      121 tgtattttca tttatattac taaacattat ctatttttat atacatttct atgtatttta
      181 ttttcatcaa atcggtctat aaattcattt ttttcttcac tatatccaat agatataagt
      241 gcataaggaa ctaattttac tggtaagtct aaaatttgtt taactttatt tactacttta
      301 tttttaggaa atactcctgc ccaacaagca gctagacctt gattagtaac ttctaataat
      361 aaattttgtg tgcaagcacc taaatcttgt atccatttac caggttttaa gaatttatct
      421 ctatttccaa gcactactat acataatggt gcaaattcta tatgctttgc tctatgctgt
      481 gactttgaga gctctttcag cagttcctta tcatctacta taataaattc ccaaggttga
      541 gcattttttg atgaaggtgc ttgcatagct gattttagta tttcagttat tttatcttct
      601 tctatatttt tatctatata tgttcttatt gaacgtctat tttttataac ttctaacata
      661 cagattctcc tttaaataaa attatttcta tttatataat atcattactt taagttttag
      721 ctattactta tataaagaat aaactttgtg ttggttttat ctaagtaaaa gtaacattca
      781 gaactaaaaa acaagactat aaatccaaga tattaattaa aaaagaagta ataaaaaata
      841 atatacaaga tttaaaaaag agtattgagc ttaaaattta attagaaaac caattttgag
      901 ataatctgtt ttttatttta acaaaaaaac ataaataaag ttcgatacgt agcgaatgaa
      961 aaaaatacaa gaaatatttt ttttaaaaaa tttcaaaaaa gagttgataa tatatgtata
     1021 ttaatataaa atttaaggtg tataattcgt ataataatac gaatgatttg tagtgtaaaa
     1081 taacttataa acggagaggg gaagtctaat gaaagtagca gtagttatgg gttcaaaatc
     1141 agattatcca aaattagaag aagggataaa actgcttgaa aaatatggaa tagaggttgt
     1201 agcaagagca ttatcagctc atagaacacc agaacaactt tcaatattcc taaaagaaat
     1261 agaagatgat actgatgtaa taatagcagc agcaggaaaa gcagcacact taccaggtgt
     1321 aatagcttca caaacattaa ttcctgtaat aggattacct attaaatcat caactatgga
     1381 tggactagac tcacttttat caatagttca aatgccaaaa ggaatcccag ttgcaacagt
     1441 tacaatagac ttaggtttaa acgcagcttt attggcatta caaataatga ctttaaaata
     1501 cccaaaatta aaagaggatt tgaaatcata tagagaagag atggcacaaa aggtgttaga
     1561 agacgacaag aatttaaggg ggtagcagaa atgttattat atgaaggaaa agcaaaacaa
     1621 gtatattcta cagataatga aaatgaatat gttgtttatt ataaagatga tgcaacagca
     1681 tttaatggag aaaaaaaagc agaaatatct tcaaaaggta tactaaataa taagatatca
     1741 acaataatct ttgaaatgtt aaaagaaaat aatataaata ctcactttat aaaaagttta
     1801 tcagatagag aaatgttagt taaaaaagta gagattttac cattagaagt aatagtaaga
     1861 aatattgctg ctggttctat ctgtaaaaga gttggtcttg aagaaggtgt agttttcgat
     1921 gagccaattt ttgaaataag ttacaaaaat gatgcttatg gtgacccaat gctaaatgat
     1981 gattatgcag tggcaatgaa gttagcaact agagaagagt taaaattctt aagagaagaa
     2041 actttaaaaa taaacgagtt actaaaagca tttttcttaa aattaaacct aaagttagtt
     2101 gactttaaaa tagaatttgg taaggattct gaaggaaata taatattggc agatgaagta
     2161 tcaccagaca cttgtagact ttgggatgta aatacaaatg aaaagttaga taaggacaga
     2221 tttagaaaag accttggaga ccttgtagaa ggttatacgg aagtactttc aagaatgaat
     2281 aataaatagg aggcaaatgt atgtgtggag ttttagggat ttactctaat aaagatgtaa
     2341 ctaaagaact atattattct ttatattcta tgcaacacag ggggcaagaa agctgtggtt
     2401 tagctctttt agatgatgga gaaattaagt acaaaaaaga tatgggatta gttggagatg
     2461 tttttaagga aaatgaatta tctaagttaa aaggaaatat agggattgga catgttagat
     2521 attcaacagc tggaggaagt catgtttcta actgtcaacc tttagttgga agttgtagaa
     2581 aaagacaatt agccatagct cataatggaa atctagttaa tgcaaattat ttaaaagata
     2641 tgctagaaga agatggatat atgttccaaa ctaattcaga tacagaagtg attttatata
     2701 tacttgctag atattacaaa ggggatatag tagaaagttt aaaagtgaca atggattata
     2761 taaaaggtgc atatgctctt gtaataatga gtcaagaaga attggttgca gttagagacc
     2821 cacatggatt cagacctctt gtacttggta aaaagggtga tgaatatata tttgcatcag
     2881 aaaattgtgc aatagatata cttggcggag aagttataag agatgtagag ccaggagaga
     2941 taatagtagt caaagatgga gaactaaaat catattttta ttcagaaaat tataaaccag
     3001 ttaagaaaag ttgtatattt gaacacatat actttgcaag aaatgatgca actatagaca
     3061 atgtaaatgc atatgaattt agaattaaat gtggtgagag attagctcag aatgaaactg
     3121 taaaggcaga tatggtagtt ccagttccag attcaggttg gccaggagct attggttatg
     3181 caaatgctag tggattaaaa ataagtgaag gcttagttaa aaatagatat gtaggaagaa
     3241 catttataaa acctacacaa gaagaaagag aaatagcagt aaaaataaaa ttaaatcctc
     3301 tttcaacaat aataaaagga aaatctataa ttttagtaga cgactcaatt gtaagaggaa
     3361 ctacatctaa acaattagta aaatctttaa gagaagcagg agcaaaagaa attcatctta
     3421 gaataacttc tcctccagta gcttactctt gttactatgg aatagatact ccaaatcgct
     3481 ctaaattaat tgcatcaagt aacaacgtag aagaaatgag agaatatatt ggatgtgata
     3541 gtttaaaatt cctagatata gaaggaatgt tagatgcaac agaacataag tctactttct
     3601 gtaaagcttg ttttgatgga gaatacccag ttaagaaaat tgataaggag gaactacttt
     3661 catgttaaca tataaagaat caggtgtaga tatagatgaa ggaaatagag cagtagacct
     3721 tataaaaggt aaaataaaag gaacttatga tggaaatgta gttggagatt taggtaactt
     3781 cagtggatta tacagtttaa aagattttgt aggtatgaaa gaaccagttt tgcttgcatc
     3841 tacagatggt gttgggacta agttaaaaat agctcagatg atggacaagc atgatactgt
     3901 tggaatagat ttagttgcaa tgtgcgtgaa tgatttaatt tgtcaaggag caaaaccatt
     3961 atttttcctt gactatatag ctcttgggaa attagtgcct gagcatatcg aaaaaatagt
     4021 tggtggaatt gcagatggtt gtaaaatgtc tggttgtgca ctaattggtg gagaaactgc
     4081 tgaaatgcct ggaatgtatg gagaagatga ctatgattta gcaggatttt ctgtaggaat
     4141 agcagacaaa gaaaagattg tttctggaaa caatgttaaa tcaggagatg tattggttgg
     4201 tatatcttca agtggagtac acagcaatgg attttctttt ataagaaaga tatttttaga
     4261 aacttacaat tataaaatgg aacaatatgt tgaagaacta ggaatgacag ttggagaagc
     4321 acttttaaca ccaactaaaa tatatgttaa attagcttta gatgtattag ctaaacatga
     4381 cataaaagct atagctcaca taactggtgg agggcttata gaaaatataa caagagttat
     4441 acctaaaggg ttaggactag atatcaataa aaaatcttgg gagaagcctc ctatatttaa
     4501 aatgatagaa ggttttaatg ctgttgatga gagagaactt cataagagtt ttaacatggg
     4561 tattgggcta gttttaatag tagataaaga aaatgctgat gatgttgtaa actttataaa
     4621 taatagagaa aatgacaatg ctgcttatgt agataaaaaa tacagtgaat tattagaaga
     4681 taaagcgtat ataattggtg aagtagtaga tagtcatgaa ggtgtagaat tatgctaaat
     4741 ataggagttt taatatctgg tggaggaaca aatttacaag cagttataga tggcactgag
     4801 tctggagaaa taaaaggtca ggttaaagta gttatctcaa gtaagcaagg tgcttatggg
     4861 ctagagagag ctaaaaatca taacataaaa gcaatttgtg aaactgatga agacaagata
     4921 atagagattc ttaaggaaaa taaaatagat ttagtagttt tggctggtta tttaaaaata
     4981 atcagcccaa agctggttaa tgagtttaga aacaaaatga taaacataca tccatcatta
     5041 attccttcat tttgtggagc tgggttctat ggagaaaaag ttcatcaagg tgttatagat
     5101 tacggagcta aagtaactgg tgctacagtt cattttgttg atgaaggtgc tgatactgga
     5161 ccaatcataa tgcaagatgt agttaaggta aatcaagatg atgatgcaaa gacacttgca
     5221 aaaagagtgt tagaagttga acataggatt ttaaaagaaa gtataagtct tttttgtgaa
     5281 aataaattaa aacttcaagg taggagagtg tttataaatg agtaagagag ctttaataag
     5341 tgtaactgac aagactggtg tagtggaatt tgctaaggag ttaaataagt tagattatga
     5401 aattatatct acaggaaata catttaaaac attaaaagaa aatggtgtta atgtaatgca
     5461 agttgaagat gttacaaatt ttccagaaat attagatggg agagtaaaaa cattaaatcc
     5521 atatatacat ggtggtatac tttataaaag agataaagaa tctcatgtag aaactgtgaa
     5581 tgagcataaa atacattcaa tagatttagt agctgtaaat ctatatgatt ttgaaggaac
     5641 tttaaaagct ggaaaaagtc atgatgaaat aattgaaaat atagatattg gtggaccatc
     5701 tatgatacgt tctgctgcca aaaactataa agatgtaata gtagtagtag atataaaaga
     5761 ttatgattct ataatagaaa aattaaaaac agatacaatg acattagaag acagaaaaaa
     5821 attatcatat aaagctttct caactacagg aagatatgat gcgttaatat ctagttattt
     5881 tgctggagaa gttggagata catatcctga catattaaat ttaactttcc aaaaagaaca
     5941 aactttaaga tatggagaaa atccacatca aaatggtttc ttatattcac aatcaaatgc
     6001 aaaaaatcca atcttaaatt atgagcaatt aggcggtaaa gaactttctt ttaacaattt
     6061 aaatgattta catggatgtt tagaagtaat gagagaattt aaagatagtg aagaagtagt
     6121 ttcagtagct ataaaacatg ctaattcatg tggggtaggt ctaggaaaag atgcttttga
     6181 agcatataca aaatgttatg aagcggataa ggtttcaata tttggaggaa tagtaggaat
     6241 aacttctact atagataaag ctacagcaga gaaattaaat gaaatattct tagagatagt
     6301 agtagcatat gattttgagc cagaagcttt agaggtactt aaacaaaaga aaaatttaag
     6361 aatattaaaa ttagctaaga tagaagatag tttacaacca tatgaaatga aatatttaga
     6421 tggaaaatta ttaatacaag atagaaataa tatattagca gaaaaaagtg aaaatgtaac
     6481 aaaagcaaaa ccaacagatg cacaacttaa ggacatggaa tttggtatga gagtagtaaa
     6541 aaatatgaaa tcaaatgcaa ttgcgatagt aaaaaatggt caaactttag ctttaggttg
     6601 tggacaaaca tcaagaattt gggcattaaa aaatgctctt gaaaataaca aagataaaga
     6661 ttttacagga gctgttttag cgtcagatgc atttttccca tttgatgatt gtgtaacatt
     6721 agctcatgaa tatggaatat cagcagtagt tcaaccaggt ggctctataa aggataaaga
     6781 ttcaatagaa gcttgtgata agtatgatat ggttatggta tttacaggaa taagacactt
     6841 caaacactaa ggaggatatt atgaagatat tggttgtagg cggtggcgga agagagcatg
     6901 ctatatgctg gaaactaagc aaagaaaaaa atgtagaaaa agtttattgc gcgccaggta
     6961 atgcaggaat tacaaatgta gcagaatgtg taaatatagg tgatactaat atagaagaac
     7021 ttttaaaatt tgccaaagaa aatgaaatag gtctgacaat agtaggacca gaagtaccac
     7081 tagttatggg aatagtagat gagtttgaaa aagaaggatt aagagtattt ggacctaata
     7141 aaaaatgtgc tcaacttgaa ggaagtaaag ctttttcaaa agaatttatg ataaaacata
     7201 acatacctac tgcaaaatat aaagaatata caaacttaga agaagctata tcagaaatag
     7261 atagttttgg atatccagta gtaataaaag cggacggatt agctgctgga aaaggtgttg
     7321 taatacctga aaatagagaa gatgctatag caactttaaa agaaatgatg tcagataaga
     7381 agtttggagc tgctggagat aaaatagtaa ttgaagagtt tttaaaagga atagaaactt
     7441 caattctagc atttgtagac aatgatacaa tagttcctat ggcaagtgca aaagaccata
     7501 aaaaagtaaa caactatgag caaggtccaa acactggggg aatgggaact ttttcaccta
     7561 gtgaaatata tacagaagaa ttagcaaata aagtaaaaga gacagtatta gaaaaaactt
     7621 tagaaggctt caaaaaagat ggacttaatt ttaaaggtat tttatttgtt ggtcttatga
     7681 taactgaaga tggagaaaaa gtacttgaat ataatgtaag atttggagac cctgagacac
     7741 aatctgtttt atttagatta gaaacagatt tacatgagat aatggaaact atactagata
     7801 ataagttaaa agatatagaa ataaactata gtgatgatga agctgtatgt gttatgctta
     7861 catcaggagg gtatccagac agttatgaaa aagggaaaat tataactggt cttgaaaact
     7921 tggatgatga tatagtagta ttccatagtg gcactaagat gcttgatggt aatttagtta
     7981 ccaatggagg aagagtaatt ggtataactg caaagtcaac tacagttaag gatgcagcag
     8041 aaaaagttta tgaaaacata aagaagataa actttgaagg aatgcactat agaacagaca
     8101 taggaagata atttttctaa aatggaggat aaggtatgtt gaatactgaa aataaagact
     8161 caatggttag aagagtatta gttgaaaaaa gagaaggttt tgaccttgag gctaaagctt
     8221 taaaaaaaga cttagtagaa agtttacaca tagataatat agaaaattta agaatattaa
     8281 atagatatga tgtagaagga atatcagaag aagtttatga aaatgcagca aagacaatat
     8341 tctctgaacc aaatttagat gtagtatatt atgaagaaat accaaaatta aatgatgaaa
     8401 gagtatttgc aatagaattt cttcctggtc aatacgacca aagaggtgat tgggcagctc
     8461 aatgtgtaca aattgtaaat caagggataa gacctgctat taatacagca aaagtttata
     8521 tattatcagg caaaataact gatgaagagt tttctaaaat aaaagattac tgtataaatc
     8581 cagtagatag tagagaagca tctttagaaa aaccagaaac attaaaaatg gaaactgaaa
     8641 taccaacaac agttgaagta ttagatggat ttatagattt agatgaaaat ggattaagaa
     8701 catttgtaag tgaaaaagga ttagcaatga ctttaggaga cttacaacac gttcaaaaat
     8761 actttaaaga tacagaaaag agaaatccaa ctataacaga aataaaagta ttagatactt
     8821 attggtctga tcattgtaga catactactt ttatgactga aatagaaaat gtaaagatag
     8881 aagatggtaa gtttaatgat atagtaaaag aagcgtatca aatgtatctt aactcaagag
     8941 acaatgtata tgtgaacaga cataaagata tatgtttaat ggatatagcc actgttgcag
     9001 taaaagaatt aaagaagaat gggaaattaa atgacctaga tgaaagtgaa gaaataaatg
     9061 catgtagtat aaacgtagat gttgaagttg atggtaagat ggaaaaatat ctagtaatgt
     9121 ttaaaaatga aactcacaac catccaactg aaatagaacc atttggtgga gcagctactt
     9181 gtttaggtgg agctataaga gacccattat caggaagaag ttatgtttat caagcaatgc
     9241 gtgttacagg aagtgctgac ccaagaacga ctttagaaga tactttacct ggaaaactta
     9301 tgcaaagaaa aataactaca gaagctgctc atggatatag ttcatatggt aaccaaatag
     9361 gtcttacaac tggacaagtt gcagaagtat atgatgaaaa ctttgttgca aagagaatgg
     9421 aaataggtgc agttatagca gcagtaccaa aagagaatgt agtaagagaa agacctgaag
     9481 caggagatgt aatagttctt cttggaggaa aaacaggaag agatggttgt ggaggggcaa
     9541 ctggttcatc taaagaacat agtgaagagt caatcttaac ttgtagtgca gaagttcaaa
     9601 aaggagatgc tcctaacgaa agaaaaatac aaagattctt tagaaataaa gaagttgctc
     9661 aaatgataaa aagatgtaat gattttggag caggtggagt ttgtgttgct ataggagaga
     9721 ttgctgatag tttagatata aacttagact tagtacctaa aaaatacgat ggacttgatg
     9781 gaactgagct tgcaatttca gagtcacaag agcgtatggc agtagctata aaaaaagaaa
     9841 ataaagataa atttatacaa ttagcagtag aagaaaactt agaagctaca catgtagcta
     9901 cagtaacaga tacaggatat ttaagaatgt tctggaatgg aaaagcaatt gtagatataa
     9961 atagagagtt cttagataca aacggagtaa aacaaactac tgatgtccat gttactaaag
    10021 tagatgaaga aaatacattc tttagctcta atgaaatagt aaaagatgtt aagtgtgcaa
    10081 gtatgaaaga caagtttaca aaagtattat ctgaccttaa tgtttgttct caaaaaggat
    10141 tagtagagat gtttgataat acaattggtg gtaatacagt acttatgcca tttggaggaa
    10201 agtatcaagc gactccaaca caaggtatgg ttgctaagat accagtactt ggaggagaaa
    10261 ctaatacttc tacaataatg acttatggat ataatccaaa ggtaggaaaa tggagtccat
    10321 tccatggtgc cttgtacgca gtagtagaat ctgtgtgtaa attggttgct attggaggaa
    10381 attatagtac aacaagactt acattccaag agtattttga aaaacttggt aacaatccag
    10441 aaaaatgggg taaaccattc tcagcacttt taggagcatt ttatgcacaa agtaaatttg
    10501 agatacctgc tataggtgga aaagacagta tgtctggtac atttaaagat atcgaagttc
    10561 caccaacatt agtttcattt gctgttgata cagtagatgc gaagaaagta gtatctccag
    10621 aatttaaaaa agcagactca aaagtagtaa tgttatgtgt aaacaaggca gaaaatgatg
    10681 ttgtagattt tgaggaatta aagagaaact tagacaaagt aagagaactt atacatggaa
    10741 ataaagtttt atcaacatat gcattaggat ttgcaggtgt aggtgaagca ataagtaaaa
    10801 tggcttttgg taataaaata ggatttaagt ttagtgaaga agctgaaaaa acatttacag
    10861 atgataaatt atttgaggct agttatggaa atatagtact tgaattagca aatgatgatt
    10921 tatgtatgtt agaaggatac aattatgttg tgcttggaag tacaactaaa gaagctagta
    10981 tatttatcaa aggtgaagaa cttgcattag atgaattata taaagcacac tgtagtactt
    11041 tagagcctat attcccaact aaaacagaag aagtaaaatc aaagatagag actataagtt
    11101 atatatcaca aggagaagct aaaaaatcat cattaagtat agctactcca agagtattta
    11161 taccagcatt tcctggaaca aactgtgaat atgactctgc aagagcattt gaaagagtag
    11221 gagctaatgc aaacataaga gtattcaaga atttaactta taaagacata gaagattcta
    11281 tagatacaat agtaaacgaa ataaaatctt ctcaaataat aatgttacca ggtggattta
    11341 gtgctggtga tgagccagat ggttctggta aatttatagc tactgtattt agaaatccta
    11401 gagtgcaaga agctataaat gaatttttaa ctcaaaaaga tggactaatg ttaggtatct
    11461 gtaatggatt ccaagtatta attaaattag gattagttcc atatggagaa ataagagttc
    11521 caagtgaaag tgctccaaca cttacttaca ataatattgg tcgtcatcaa gctaagattg
    11581 ctagaactag aatttcatct aataaatctc cttggttagc tcaaactaat gttggagata
    11641 ttcataatat agcaatttct catggagaag gtaaatttgt tgctagtgaa gatgttatga
    11701 gagaattaat tgctaatgga caagtagcaa ctcaatatgt tgactttaat aatgaagcta
    11761 cttatgatat tgagtttaat ccaaatggtt cattctatgc tgttgaaggt ataactagtg
    11821 ctgatggtag agtgtttggt aaaatgggac attctgagag aattggtgag gaagtattta
    11881 agaatattat aggtgaaaaa gaccaaaaga tatttgaatc tggagttaaa tattttagat
    11941 aaagctttga tgtttaattg atagctagaa tatataattt agatatttag atttaaagag
    12001 atgtaataat atgatgataa tcatttaata ttacatctct tttttattta tctaagaaat
    12061 aataagttca acctaagtta aaatacaagc tgtgttataa ttaattacta agtaaaatta
    12121 aatatttatt ggagggcaac ataattaatg aaaatattaa taacagggtc taatggtcaa
    12181 ttaggaaaag aattggttag ccaattagaa gcaatcaatc aaagtataaa tcaaccaaaa
    12241 tacgttatat tagctacaac aagaaatgat ttggatatat ccaatcagac taatatagat
    12301 aattttattt tgcataataa acctgatgta attgtaaact gtgcagcata tacaaaggtt
    12361 gatgcatgtg aagataatat cgaaattgcg tataaaataa atgccttagg agtgagaaat
    12421 ttagcaatag catcagaaaa agttaatgct aagcttgttc acatatcaac tgactatgta
    12481 ttcaatggtt tttctaaata tccatatagg gaagacaata aaactgaacc caattctgtg
    12541 tatggaaaaa gtaaacttat gggagaaaag tttgttgaac aattctcaca taagtacttt
    12601 atattgagaa cagcatggtt atatggtaat gggaataatt ttgtaaagac tatgataaaa
    12661 ctttctttag aaaataaaga ggttaatgtg gttaatgacc agtttggaac cccaacaagt
    12721 acagtagact tagcaaaaat tataataaga atcatggaaa cagaacatta tggagtttat
    12781 catgctacat gtgaaggtga gtgtagttgg tatgattttg ccaaaaagat atttgaatta
    12841 aaaaatatag atataaaagt aaatccaatt aagagtaacg aatttaaatg taaggctcaa
    12901 agaccactat attctgtatt ggataatttt atgttgaagt taattggttt aaattctttt
    12961 agaaagtggg aagaatccat tgaagaatat ttagaaagat atgatataaa tgaaaagtaa
    13021 atgtattatt tttagaaaag aaaataagat attataaaaa atataaatat ttcttttatt
    13081 tatccgataa tcataatatg acaaaaacaa atttaaggat ggaaaaagat gaaaggaatt
    13141 attttagcag gagggtctgg tacaagacta tatcctataa caaaatgtat atcaaaacaa
    13201 acacttccaa tatacgataa accaatgata tattacccta tgtcggttct tatgttggca
    13261 gggatacaag atatattaat aatatctaca ccaagagata taaaagcatt tgaagaatta
    13321 tttggagatg gaagttcact agggcttaaa ataacatatg caattcagga gaaagcaaat
    13381 ggaattgcag aagcgtttat aataggagaa gaatttatag gaaatgacag agtgtctctt
    13441 gtattggggg ataatatatt ttatggatat ggatttagtg aaaggttgga gaatgcagtt
    13501 aaaagaaaag gagcaactat atttgggtat catgtaagta atccaaattc gtttggagtt
    13561 gtagaatttg ataaggattt taatgtatta tcaatagaag aaaaacctac agttcctaaa
    13621 tctaattatg ctataccagg attatatttt tatgataatg atgttgtaga gatagcaaag
    13681 ctagttgaac catcagatag aggtgaatta gaaataacct ctataaacag tgaatatcta
    13741 aaacgaggaa aactaaaagt ggaacttcta ggaagaggta tggcttggct tgatactggt
    13801 acacataaag gtctattgga tgcttctaac tatgttgaag tggttcaaac aagacaggga
    13861 ctgtatatag catgtttaga agagattgca tatacaaaag gatatataag tagagaacaa
    13921 ttaatgaaac tagccaagcc actgataaaa actgaatatg gtcaatatct attaaaattg
    13981 gctagtgaag atatgcaata tggtagataa aatttattat aaattttatt aagggagttt
    14041 gttataatgg gaaaaatcaa tgttataaag actaaaatag aaggattata tatagtcgaa
    14101 ccaaatgtat tttgtgatga aagaggatat tttatggaaa gctataataa aaaggatttt
    14161 atagagcagg gtcttgatat gtgttttatt caagataatg agtctagctc aagaaaaggt
    14221 gttttaagag gaatgcattt tcaggttaga tatcctcaag gaaaattagt cagagttaca
    14281 gagggagaag ttttcgatgt tgcagttgat ttaagaaaag attcacagac atttgggaaa
    14341 tgggaaggtg taatattgtc tgataagaat aaaaaacaat tttatattcc agaagggttt
    14401 gcacatggat ttttagtttt atcagaaaaa gcagtattta attacaaatg tacaaactat
    14461 tattatccag aatttgagca tgggtttata tggaatgaca agcaaatatc tattgagtgg
    14521 cctttaaaag gaattgaaga aatattttta tcagaaaagg acaaaaaaca aaaaagtttt
    14581 aaagatattt gtataagacg ttaataaaaa tttattagaa ttaacattta ggagaaaaaa
    14641 tatgaaaaaa atactgataa ctggaggagc aggatttatt gggagtaact ttgtacatta
    14701 tatgcttaac aagtatgtag attattttat tgttaaccta gacttgttga cttatgctgg
    14761 taatttagag acattaatag aaatacaaga cagttccaat tataaatttg ttaaaggaga
    14821 tatttcaaat agggaacaga tgtataagct atttgaagaa gaaaggtttg atatagttgt
    14881 taactttgcc gctgaatctc atgtagatag gtcagttgag aatcctgatt tatttattaa
    14941 aacaaacatt ataggaacac aagtactttt agatgcatct ataaaatatg gagttaaaag
    15001 gtatcatcaa atatctacag acgaagtgta tggtgattta ccaattgata gaaaggattt
    15061 attctttaca gaacaatctc ctatcaatcc aagttctcca tattcagctt ctaaagcatc
    15121 agcagatttg ttggtaagtt cttattatag aacatatgga ttgttaacaa ctatatctag
    15181 gtgttccaac aattatgggc catatcattt tcctgaaaaa ttgatacctc ttatgatttt
    15241 aaatgctttg gaaaacaaac aattgccagt atatgggaat ggggaaaatg taagagattg
    15301 gttacatgta tatgaccatt gtactgctat agacttaata atacataaag ggaatatagg
    15361 ggagatttac aatataggag gtcataatga aagaagtaat ttagaagtag taaaaatgat
    15421 attaaatctt ttagataaat ctgaagagtt aatatcctat gtaaatgata gacctggtca
    15481 tgatttaaga tatgctattg atgcaactaa aatagagaat gagttaggtt ggaaggcaaa
    15541 atatgacttt gatttgggta taaaagaaac tgttaagtgg tatatagaaa atgaatcttg
    15601 gtggaaagct gttttatcag tgtaactttg aaatttataa aatttagaaa ggggcgtttt
    15661 atattgaatg agataagttc actaattaat atattagcat tgactcaatt atctggtagt
    15721 aatactacaa atcaatgtaa ctgtacatat aatagttcat ctggatttga tatgattatg
    15781 atgaccttgt taaaagcatt atcacaaaat aatcaacaga cttctaatgg ctacaattta
    15841 tcgtctaatt ctgagaattt atttgacgaa ctcggcagtg tagtaaataa tacagctagt
    15901 agatttatag atgttgatac aaaagataaa agtgtaaaat caagaattga aaatgctata
    15961 gagcaagcat caaaaaaata taatgtggat tcgaatctta taaaagcaat aattaaagtt
    16021 gaatcggatt ttaatccaaa tactgtttct tctgctggtg ctaagggtct tatgcaactt
    16081 atgccagaaa attgcagaga cttgggggtt acagaccctt ttaatataga gcagaatata
    16141 gatgctggaa ctagacatat taaggaatac attgatatgt ttggagggag catagaaatg
    16201 ggattgatgg catataatgg tggtcctgga agaatgaaaa gtagaggagt agaatcaatc
    16261 tctgacctgt ataaaatgcc aaaagaaaca caaaattata ttcctaaggt tatgaaatat
    16321 tatagagggt aggctttatt atggggataa atatagataa gctaaacata agtccaagta
    16381 taaatatgga ggttaaaaaa ctaaaagaga gtaataaatt ttctgacaaa ttaaactctt
    16441 taaaacaatc ttcttcattg gaagatataa aacgaaattt ggaaagtgta aaaaaggcag
    16501 gagaaaggct tgtacttact caaaactatg gcgacattag tatatataaa aatgcagtaa
    16561 aagaatacct aaaatctgtt gtagataata tgtattcact tgataaaaat agtagctttt
    16621 gggagcatca gtattataag aatgtaaaga ttgttgatag taaacttgag gatatgacta
    16681 gtaagttaat tagtgaagaa aaagaaaaca tagatattgt ttctactgtt gatatgatac
    16741 aaggcttgtt gatagatatg tatagataag gttatacaag gagaaaagtt ttatgaatag
    16801 taaaaaagag gatatatatg aattgaattt tgggcaatta atagaagaaa aatctggtgt
    16861 tatagaagaa aatcaaaatg tttttaatgc aaaagtaaat gtagctgttt ctataggaaa
    16921 tacaacagaa acaataaaaa atatattaga tttaaaagaa ggctctataa taaaactaag
    16981 taagaatgtg gatgaaaaat tagatatata tgcaaatgat agattgtttg cttatggaga
    17041 aagcattatt acaaatgaga agttatctgt aagattatct aaaatagaag attcagatgt
    17101 aaattagtat tttaaatagt atccctttag gctatgatgt atgagtttag ctaaggggat
    17161 atttttataa aaaatttaaa atatgcgtga attatataaa tgtttttatt ttatgagcga
    17221 taattaaatt ataagtatgt aattttggag gtgaaactat ggatattaaa aatgtaagtt
    17281 caaatattgt agtgaataat tatttaaaga atacatctgg tgtaaaaaaa gtggaagaag
    17341 ttaaaaaaat taataaaaca gacaagatag agctatctaa atttgcaaag gaaatggcaa
    17401 gtgtgtcaaa tatgagtgat gagaatatta ggaataaaaa agtcgatgaa ataaaaaatg
    17461 cattagaaaa tggaacttac aaaataaaac caaaagaaat tgcgaaaaaa atgatagagg
    17521 atatgcgagg ataaagtcat gattacagac ttaaaagtta taatatatga agaaaaaaat
    17581 ataattagta agatgatgga attattaaaa gaacaatttg attatatagt tgaaaaagac
    17641 ttagaaaatt taaatatgtt aaatcctaaa ctagaagata ttagcaggga gcttgcatcc
    17701 atagaaataa agagaagaca actttttggt gatgatattt caatgagtga agttgttgaa
    17761 aatagtaatg atagatattt aaaagaaata tatacagact tgaaacacat tcttgcttta
    17821 gctaaaaatc aacaagaatc aaatgattct ctaataaaga aagaacttat ttttacgaaa
    17881 aaaatgatta atttcataaa accagctgat aagcaaactg caacttataa ttcttatgga
    17941 aatattagaa aataaatatt aggaggagaa atatggcagg attattttcc attttaaata
    18001 cagctaattc aggaatgaat gcgcaccaaa aagcaataca gacaatttct cataatataa
    18061 gtaatctaga tacagacggt tattctaggc aaaggactga gtttgctaca aatagtccaa
    18121 tgtatatgcc tagtttgagt aattcaattg caagaggaca attagggact ggagtacacg
    18181 taacggatgt aacaagagct agaaatagtt tttatgatta ccaatttaga gcagaagctc
    18241 ataagtatgg taatacagtt tcaaagtatg actattataa tactatagaa actataataa
    18301 atgaaccatc agactatgga atatctgcag gtatagacga cttttttaaa ggatgggaat
    18361 cattagcaaa ggatataaat agtgatagta aaaaaagtct agtggtagaa aatgcatcaa
    18421 atctagcaac tttaataagt gaaagttata agaagcttaa taagttaaag ggagatatag
    18481 acgataatat aaactctgaa ataaaacaga taaatgacat gttaaaaagt cttgaagatt
    18541 tgaataaatc tattgatgtt atatctggtt ctggctctac tccaaatgat ttacttgatg
    18601 aaagagatag aatattagat gacttaagct ttaaattaga ccttgaaaat agcgatgtta
    18661 aaaatatgct ttcagatggt aaattagaac ttaatgaatt aaaaaatgca gatggaactt
    18721 ggaaaacagg tgtatcagga accttacaag gcttgtttga aatgcatggt aagattgata
    18781 catataaaag cgatttaaaa gatgtatctg atgggttagc taagcagata aatgatgttt
    18841 ataatacatc tgctggaata acagtaacag atttttttat aacttcaaat gtagatggtg
    18901 aagatatcat aaaggtaaat ccagctataa agagtaaccc aaatgaatta aaacttactg
    18961 ctgatgaggc ttcaaagatt gctaagttaa aggatgaaaa aatagatatt ggggtggctg
    19021 gtggaaaagt aagtacaata agtgaccact ataaagcctt tgcagaatca gtaggtttag
    19081 attctcaaaa agtgaatcaa gatgaagtga atcaacgtaa aattataaat aatattgata
    19141 actcacgaat gagtgtttca ggcgtatctt tggatgaaga aatgacagag cttataaaaa
    19201 tacaacgctc ctatcaagct agtgcaaaag ttatgtcaac agctgtgcaa ctgttggatg
    19261 ttgtaataaa tggaataatt taatagaagg gggaatttat aatgagagtt agtacaggta
    19321 tgatgtctag caactatcta aactcactac aagataatct acaaagatta gataaggtta
    19381 atagacaagt taatacaacg aaagaaataa acaagctatc agataatcca tataaggcaa
    19441 taaaaatatt gaactcaaaa agtgaaataa aaactatgga gacttatgtt gagaattgta
    19501 aagatacagg gaattggtta gaaactactg atactgcatt agatcagctt ggaaaattat
    19561 tatcggacat aaagaaaact ttggtggcaa gtggtgatgg tgcatattca gatgatgagc
    19621 taaagactct tagtaatgaa actaatgaaa agatgaaaga aattgcaaat gccttaaatg
    19681 caacacatga aggaaagtac atattttcag gttcaaatac aggtgtacca cctgttgaat
    19741 gtatagataa tgctgacggt tcagtgagct taaagtttaa ttctagttta aatcttaata
    19801 aattaaatga ctctttgagt atggatatag ctcaaggaat aagtgtagat tataatgtta
    19861 aattgagtag ccttggattt gacccaacaa agactgaccc atttgaagag ttaaataata
    19921 tatcaaagaa attagcaaat ccagatgctg atgctataaa agagctgaca acaacttgtt
    19981 taggcgacat ggaacaatta atagaaaata cagtaaatgt taggtctata tatgggacaa
    20041 aagcaaatac agtagaggct atgaaggaaa aaaatgatga tagtttgata cagttaaagg
    20101 atattttgtc acaaaatgaa gaaatagatt atggagaaaa actcgttcaa ttaaaggcag
    20161 cagagttaac ttatcaagct tcactacaaa caggaggaaa gttatttaat gtatctatat
    20221 tagattacat atagtaaaat aacgcagata tgatgaaggt tacattaaaa aaaggtatat
    20281 taggttttga aaatttaaaa gaatatgagc ttttagatat agaagataat gattctttaa
    20341 aagaatttaa ttctactgaa gaagaaggta taggatttgt tgttgtatct ccatttgaga
    20401 ttatcaatga atatgaaata gttttaaatc aggaaacaat agaaaaacta gaagtgaaat
    20461 ctccaaagga tgttgtacta tttaatataa ttactcttgg tcagacattt gaagaatcaa
    20521 ctgtgaacat gaaagctcct atagtgataa atataaaaac taattttgga atgcaaataa
    20581 ttttacagga tgataaatat tcaataaggc atccattatt gagaggtgat aatggatgct
    20641 agtaatttca agaaaaaaag atgaagcagt acttatagga gaaagcatag aagtaaaaat
    20701 tgtaggggta gatggaaaca atgtgaaact agctatatcg gcgcctaaca atataagtat
    20761 attaagaaaa gaaatatacg aaaaagtaaa gagtgagaat ataaaagcta caaataaaaa
    20821 tataaaaatt ttaaagtcat taaaataata agcttggagg aagtaatata tgtatggaga
    20881 aaatccttat aattcttata aacaaaatgc tgtttttatg gcatcaaagg aacaattgtt
    20941 acttatgctt gttgatggag cagtaaaata taccaaaata gccagagcag ctatcttaga
    21001 taaaaataca cagaaagcgc atagagaact tgtaagagtt caggatatat ttacagaact
    21061 aatggttaca ttagaccaaa atgcaggaca atgggcaaag gatatgtaca aagtttatga
    21121 ttttgtaaga tatgaacttt caagagccaa tattagaaaa gacatccagg taatagataa
    21181 tgtgttgcct gtaatagaac aaattaaaga cacatggcat gaagctgata aaaaaagtaa
    21241 agaagaaaga agtaggtaca aataatggaa ttaaatttag ctgaattaga taagttatca
    21301 aaagaggaat tactattgat gcttgtagat ggcacagtaa aatataccaa tatatccaaa
    21361 gaagctcttt taaataacga ctatttgaaa gcacataatg aacttataag agttcaaaat
    21421 atatttacag aactaatgac tacattagac caagatgcag gtcaatgggc aaaggatatg
    21481 tataaagttt atgaatttat aaaatctgaa ttagcaatag ctgatgaaaa taaagatatt
    21541 aaaataattg atgatatatt gccaatagta aaacaaatta gagatacatg gcatgaagtt
    21601 tacaatcaac taaaaatata attagatgga gggataaagg tatgtcaagt ataagtccta
    21661 taagagttac aggtctttca ggaaattttg atatggaagg cataatcgaa gctagtatga
    21721 ttagagacaa ggaaaaagtt gataaagcaa aacaagaaca acaaatcgtt aaatggaagc
    21781 aagaaatata tagaaatgtt atacaagaat caaaagatct ttatgataaa tatctaagcg
    21841 taaattctcc taatagtata gtaagtgaaa aagcatactc ttctacaaga ataaccagtt
    21901 ctgatgaaag tattatagta gcaaaaggct cagctggtgc agaaaaaata aattatcaat
    21961 ttgcagtttc tcaaatggct gaaccagcaa aatttactat taaattaaat tcaagtgaac
    22021 ctattgttcg acagttccct ccaaatgcca gtggagctag ttctttaact ataggagatg
    22081 taaatatacc aatatctgaa caagatacta caagtactat tgtaagtaag ataaactccc
    22141 tttgcgcaga taatgatata aaggcttctt atagtgagat gacaggtgaa ttgattattt
    22201 cgagaaaaca aactggttcg tcatcagaca ttaatttaaa agtaattgga aatgacaatt
    22261 tagctcagca aattgctaat gataatggta tcacatttgc aaatgatgct agtggaaaca
    22321 aagtggcaag tgtatatgga aaaaatctag aagctgatgt aactgatgaa catggaagag
    22381 taactcatat aagtaaagaa caaaattcat ttaatataga taatattgac tataatgtaa
    22441 attcaaaagg aactgcaaag ttgacttctg tcactgatac tgaagaagct gttaaaaata
    22501 tgcaagcatt tgtggatgat tataataaac tgatggacaa ggtctatggt ttagttacta
    22561 ctaaaaaacc aaaagattat ccgcctctta cagatgccca aaaagaagat atgacaactg
    22621 aagaaataga aaaatgggaa aagaaagcta aagaaggtat acttagaaat gatgatgagt
    22681 taagaggttt tgttgaagat attcagtctg cattttttgg agatggaaaa aatattattg
    22741 cattaagaaa actaggtatc aatgaaagcg aaaattacaa taaaaaaggt caaatatcat
    22801 ttaatgcaga tactttttca aaggctctta tagatgatag tgataaggta tacaaaacac
    22861 tagcaggtta ttcttcgaat tatgatgata agggaatgtt tgaaaagcta aaagatattg
    22921 tatatgaata ttctggaagt tcaacttcta aacttcctaa aaaagcaggt atagaaaaaa
    22981 ctgcttctgc tagtgaaaat gtatattcaa aacaaattgc agagcaagaa agaaatataa
    23041 gcaggttagt tgaaaaaatg aatgataaag agaaaagact ttatgctaaa tattcagcct
    23101 tagaatcttt gttgaatcag tattcttccc aaatgaatta tttctcacaa gcacagggta
    23161 attaattatg gataaattaa aagaaaaatt aaatttatat aaagatatat ctttacaaat
    23221 tataaatttg attgaaaagg aagaatatat aaagataagt agtaagttag gtgaaagaca
    23281 agagattata aatagtgtaa gtgaaattga tagaaatgat tttatacagc tttataatag
    23341 aatggaatta attgaaattg atagtaggat aagagatatt ttacaaggac aattattaga
    23401 agtaaaaaaa gaattacatg agtataagct tactaaacaa gtgaacacaa tgtattataa
    23461 cttaaataga gaaaaagtaa atatttttaa taaaaaagta taaagttatg taccatcttg
    23521 tccgattata taaatgaaga attaataaaa aagattaaaa gtagaaatga caaggatgtc
    23581 aactatacta aggagggtaa aataatgaga gttaatacaa atgtaagtgc tttgatagca
    23641 aataaccaaa tgggaagaaa cgtaaatgca caaagtaagt ctatggagaa gttatcttct
    23701 ggtgttagaa ttaagagagc tgctgatgat gctgctgggc ttgctatatc tgagaaaatg
    23761 agagctcaaa taaaaggttt agaccaagca ggaagaaacg ttcaagatgg tatatctgtt
    23821 gtacaaacag cagaaggtgc attagaagaa actggtaata tactacaaag aatgagaact
    23881 ttatcagtac aatcttcaaa tgaaactaat actgctgaag aaagacaaaa gatagctgat
    23941 gagttgttac aattaaagga tgaagttgaa agaatatcaa gctcaattga gtttaatggt
    24001 aagaaattac ttgatggaag ttctacagaa ataagattac aagttggagc taattttgga
    24061 acaaatgttg caggaacaac taacaacaac aatgaaataa aagttgctct agtaaatact
    24121 tcaagtataa tgagtaaagc aggtataaca agttctacaa tagcaagctt aaatgcagat
    24181 ggaacttctg gaacagatgc agctaaacaa atggtgtcta gcttagatgt agctcttaaa
    24241 gaattaaata catcaagagc aaaattaggg gcacaacaaa atagactaga atcaactcaa
    24301 aacaacttaa ataatactat agaaaatgtt acagcagctg aatcaagaat aagagataca
    24361 gatgttgctt cagaaatggt taacttatct aaaatgaata tattagttca agcatcacag
    24421 tcaatgcttg ctcaagctaa tcagcaacca caaggagttt tacaattatt aggataaaat
    24481 aattttgtat attggatata gaagaaaggg taaggctttg ctttactcct ttcttttttt
    24541 acaaatatac ttttgaaagg aaaatatata tgttacttag tatagttatg attgttaaaa
    24601 atgaagaaaa aatacttgaa aaaactttaa aatcattaac cacattaaga agttctatag
    24661 agagtgaact tataatagtt gatacaggtt ctacagatga tactataaat atatctagga
    24721 aatacacgga aaatgtgtac tttcataatt ggaataatga tttttctagt atgagaaata
    24781 tatcaatatc ctatgctaaa ggtgaatggt tacttatatt agatgcagat gaaatactaa
    24841 ttgattcttc aactattgtg aaatttttta aagatggtct tgataaaaaa tttaattcgg
    24901 cctcaataca attaaaaaat ctttactcat atgataaaaa attatatgga tattgctcag
    24961 tgcttagact ttttaaaaac atcgatttta gataccatgg taaagtacat gaacaaccaa
    25021 tatacaaaaa ccctatattt aataatgttg ctgattttga acattatgga tatttgtttg
    25081 aagatgaaga aataagatta aataaagtaa aaagaaatga aaaactgtta tttgaagaat
    25141 taaaagagaa tgataattct ccatatacaa attatcaatt agggaaaaat tttattatat
    25201 taggaaaata tcatgaggct ttatactatc ttgagaaatc ttatgaatta tattctaaac
    25261 tagaatcggc acctggttac ttgataacaa gtcttgtaaa aacatattta tatttaggaa
    25321 agcataagaa gtgtgaaaaa ttgtgtctaa agtatattaa gaaagacatg aataatattg
    25381 atatatatta ttatattgca caagcacaag tagatttagg gaagtatgaa aatagtatag
    25441 attcgtataa gagatatatt tatttgttag ataattatga aataagtaca caagcaaata
    25501 gtttattttc agacactgat actgtagggc ttagggatga agctattatt acactgataa
    25561 agatttatta taaacttgaa aaatatgagt ctgtaatttc agagtacaat aacattgagg
    25621 atatggaaaa gaagaagaat gtgtacttta gcttatttat gtccctatac aaattgaaca
    25681 gatttgaaga tataaaaaac tattataaag aagtttcaaa ttctaaaatt gaaaaaaata
    25741 gtttttataa aaatatagaa gaagttataa ataatattaa agatgatgaa aaagagcatg
    25801 tatataaaat cctttgtgac attgaaggta attatggcat tttaaatcag ataaggataa
    25861 ataaatgtgt atctatagat aagtgtaaag aaatactcaa tgaagaaaaa gaaataattt
    25921 atgcatatat tataaaagta gcttttgaag ataagattga tttactagat attttttatg
    25981 aaatggaata tgtatggata gagaaatacc taaaatatct tttagcacta gaaagaagtt
    26041 ttaatttagt actatataag tacataatta gtcgtccgaa tacatcagat ataaaaaaga
    26101 taagagtata taaagctata tctaaagtag ttttagaaag tataagttta agtgatgaaa
    26161 aatataagga gattttttat ctttatataa tgtattctta taagtacata agatacatat
    26221 atagaaattt aagcgatttt gaattaataa aatttgtttg caatgattta gataagttta
    26281 ctatagaatt taagcatcta cttgacatta aaaataaagt aaatgatgct aatgaaaaat
    26341 tagagtatat acatagtatg aggaaattac taaatgagta tcctttttat aataaaataa
    26401 taaaaatact tataagtgaa ttagaagaaa atataaaaga aagtgaagaa tttaaggatt
    26461 taaaagatag ttttattatt agtatagaaa atatgattga aactggcaaa ataaatgatg
    26521 cgaaatcatt ggtagatgac tattcaaaaa catttatgga tgatgtaaaa attttaaata
    26581 taaagggaat tttatatatg tttgaaaata agcttgataa agctgatttt atgttaaaaa
    26641 aggcattatc attagatgta gaaaatgaag atactatata taatatagaa tacttaaaaa
    26701 gtttaagata agtcaaaaat gggagaattg aagaatgaat acaccaatag tggttaatga
    26761 aaaatctaaa gatataataa aagattggca atatacgtat acaaacgaag atataaaatt
    26821 tacaagcata ataatattga catataatca aatagaatat actaaattat gtattgaaag
    26881 tattaggaag tttaccccaa agaataaata tgaaataata ataattgaca ataactcttc
    26941 tgatggaaca gttgagtggt taaagggaca atctgacata aaatctgtat ataataacaa
    27001 aaacctagga tttccaaaag gatgcaatca aggtattgag ttggctactg gtgaaaacat
    27061 attgttgtta aataatgatg taatagttac tccaaattgg ctttataatt tgaataaagc
    27121 tttatggtct caagataata taggtgctgt aggtcctatt acaaatgcat gttcgtacta
    27181 tcaacaaatt gatgttgatt ataaatcaat tgaggatatg tttatgtttg cacacaaaat
    27241 aaatacatct aatattaacc tatggaaata tagattaaag ttagttggat tttgcatgtt
    27301 aataaaaaaa tctgtgctgg ataatgtagg ggttttagat gaattattca cacctggaaa
    27361 ttttgaagat gatgatttat cttttaggat gctagagtca ggatataagc tattattatg
    27421 ccaggatact tttatacacc attttggaag tgtatctttt aaagaaaatc gtaataaata
    27481 tacagatata atgtatatta atagaagaaa atttgaaact aagtggggat ttaatccaga
    27541 atatagtgga atgataagaa cagatttgat ttctaaaata gatgatagtt tggaggcaaa
    27601 tattaatgtt ttagaaatag gctgtggtat aggggctact cttttagaaa taaaaaatat
    27661 atataaaaat gcatcaatat atggaataga aattagtgag tcagcaggta aacttgcaaa
    27721 gtatagtgca gatgtactta tagcagatat agaaaaagta aagttggatt atgaaaaaga
    27781 tttttttgat tatataatat tgggagatgt acttgaacat ttaactgacc catggaagat
    27841 agtcaatgaa ctgaaacaat atctaaagaa ggatggttgt ttgatatcta gtataccaaa
    27901 tattatgcat gtttctgttt taagaaacct tgtgttaggt aattttacat atgaagattc
    27961 aggaatttta gacaagactc atttaaggtt ttttacatta aatgagataa caaaaatgtt
    28021 taatgatagt aattacataa taaatagtgt tacagctact agtatgccac tttcggaaat
    28081 tgataataga ttcattgata caatgtgtaa ggaaagctgt aaagaaatga gacaacagta
    28141 cgaatcttat caatatatag taaaagccac taaaagcata gatactaaaa gatattctag
    28201 tattagtatg gttaatttaa aatacgcttt aatgagaatt gataatgaat tagatgtaga
    28261 tagtaattta agttatatat ttgataatta taatataact gaaccatgtt ttgttgatga
    28321 tattgaatat ttagttgata cgtttgtgat aaataaagca actctattaa atagattagg
    28381 agttgaggca tttaacagag gtttaggaga ttttagtata aacatgttta tgacagtatt
    28441 agagatggat agagatgata ttgattcaat atataatata acaagtgtat tatgtgaatt
    28501 agaagaattt gatactgctt acaacttaat taaaaatagt agcaaaaaag tacagagtga
    28561 aagtgacata caagatatac tttattttat tgaggggaag agatatgaat aatgataaga
    28621 tatgttttat aacctgtgta aatgatgaaa gactttatga ggaatgtctt gtatatataa
    28681 atagcctaga cattcctaaa aactttagca tagaaacagt agctttaaga ggagccgatt
    28741 ctattgcctc tgcttataat aaagctatga gagaaacaga ttctaagtat aaggtatact
    28801 tacatcaaga tgcttttata attaataaaa atttcatata tgacatttta gagatattta
    28861 aaaaaaatac agatgtagga atgattggtg tagctggagt aaaagatata cctgtgaatg
    28921 gaatttggtg ggattctgat aaaaaagcag ggaaagtata tgatagccat acaggtaaaa
    28981 tggatattta tgaatttagt aactttgaag aatgttttaa tgaagttatg gctatagatg
    29041 gattaattat gattactcaa tatgatgtat tttggagaga ggataagttt gatgggtggc
    29101 atttttatga tttgtcacag agtatggaat ttagacaaaa taaatacaaa gttattgtac
    29161 caaaacaaaa taagccttgg tgtatgcatg attctggagt agcaaatata aataatggat
    29221 atgatgaata taggataaaa tttgtaaaag agtacataaa tcttgattta tatagagaaa
    29281 atttttatga atttgataag aagaaagcaa gtaccatact taagagtaat aagcatgtaa
    29341 tggattgtat gaaagaggag cttacagaac aattaagatt aaagaattat aaggatgttt
    29401 tagatattgt ttctaaaatt gctcattttt ctgtttttaa tcatactggc ttgtattctt
    29461 gtgaaaaatg tgagagagca ttgctagaat gtgcaaataa tttaccagaa atagaaaata
    29521 cgaaattatt tacaaaaaaa gatttaaaaa gaagaaaagt attacatgtt ttaagtgaag
    29581 gatattcaac aggagggcat acaagactag ttaaaaattg gattgagatt gacaaagatt
    29641 ctgttcattc agttgtaact acatggcaat cagatacact gccagactgg ttattaaaag
    29701 caatagatga tagtggtggc gaagttatat ctttggattt aatttcagat gattatttaa
    29761 aaagagctgt agctttgagg aaaatagctt atgactgggc tgatatagtt attttatatg
    29821 ttcatatgtg ggatacaata cctgttgttg cttttggagt tgatgggaaa atacctattt
    29881 tacatgttaa tcactatgat agtacttttt ggttagggac aagtattgca gatattgtca
    29941 ttgattttag cgaagctagt caattaatta cattagataa aagaggcata gataaatctg
    30001 aaatattacc aatgccatta aaagaaaaag aagtattttc aaaatctgaa gttagaaaac
    30061 gatataattt ggaggatgat gtgacaataa tattgactat tgctagtgaa agaaaataca
    30121 atagtgtaaa tgatattaat tactttaaaa ttgctaaaac attgattgaa aatacagata
    30181 atacaattat tattgttgta ggagttgata cagagaatct ctattggaaa actttaagta
    30241 acgttacaga taatagagtt atatgttttg gaacaacagc aaatatagac gatttttata
    30301 agatttcgga tatataccta gagtctttca tggtagggag tcatacttca aaattagatg
    30361 caattttata tgatttaatt cctgttaagt ttaaaaatga agtttcacca atttttacaa
    30421 atatgtggaa ttttatagat aagtttgatt ataagaatat aaaagaggtt atagacatga
    30481 taagaaggta caatactagt gaatttgaca gggataagat aaaagaaaaa caatcagaaa
    30541 taaaacattt tgttatagat aaacactgta ttgaaataaa aaactatata gacaaaatat
    30601 ataaagaggc taatcatcat aaagttaagt atgatttata taaaaatgat gaaatcgaag
    30661 actatcagct ttttgttgca ttatatggtt atatggtaaa aaataaaaac atgtcatagg
    30721 tgataaaatg aacaatattc atatcaataa atttgaaaaa agctgtgaaa aaaactggga
    30781 ttattttgta gaaaataaat ctataaatgg tacatttctg caaactagaa atttccttaa
    30841 ttatcatgaa gaaggaaaat tcactgacca ctcattaata atatataaag gtgaagaaat
    30901 actagcagtc gtacctgcat gtgaaactta tgaaaatggt gaaagagtat ttttttctca
    30961 tttaggaagc acttttggag gtattataat aagaagtggt tttaatgata ttaggcatgt
    31021 aaatgctatt acaaataagt tagaaacata tttacaggaa caaagtttca ataaagtata
    31081 tttaaaaaat acatcaagaa tattttgtaa agaggatact tcattactag aatattttct
    31141 atttaagaat aattacatgt tttatgatga attaagtatg tttttagaat taagtaagta
    31201 taaaaaagat atattagcta attttagata tagtaaaaga agatatttta aatattcttt
    31261 gaagaataat cttaccttta aaagattgta tagtgatgag gatatagttt gtttttttaa
    31321 tttactttct aattgtttag atagtaaagg agctaaagta atacattcag tagaagaact
    31381 tttggagttt aaaaataata gattaaaaga tattgtagaa ttttatgcag tatatattga
    31441 tgacattatg atttgtggga gcatggtatt taactttcaa gaaagagttt atcatactca
    31501 atatctagca tcaaattcga catataataa atattatcca atgatatttt tatactataa
    31561 tctaataaaa gatgctttag agagaaattt taagtatctt tcttttggaa ttagcactct
    31621 aaatagaggc aaatacttag acgagggttt agctacattt aaagaaggtt ttggttcaag
    31681 tgcaatttgc aacagaacat actataaaaa tataaataag tgataatcat gttttataga
    31741 gtttttagag tatagtaaat atctagtact ctaatattac ataaaaataa ttagtttata
    31801 aagaaggttt gtatatgaaa agattaatga ttttaggctc aattaagtat tttgagaata
    31861 ttgttatgtc agctaaaaaa gagggaatat acacaattgt ttgtgataat agaattgata
    31921 ctcctgcgaa aaagatttgt gatgaagcaa tagatgttga tgtatttgat tttgataaaa
    31981 taaaaagtat agctatagat aaaaagatag atggaatttt aactggattt acagattctt
    32041 taatgaaacc atatgtttat attgcaaatg aactaaattt accatgtgtg atttcttgca
    32101 atcaacttga atctgtaact aataaggcta ttatgaaaaa atattttaaa aataatgcaa
    32161 tacctacaac tgattattgt attattgaaa gtctaaaaga tttagaaaaa atacaaggat
    32221 taaattttcc acttgtaata aaacctgttg atggttatgg ttcaagaggt gtatattttg
    32281 tatcaaatat agaagaatta atagaaaaat ttgaatattc taaggaagcc tcacaagatg
    32341 gacaagtaat aattgaggaa ttttatgaaa gtgaggaaat acaaggacta gcttgggttc
    32401 ataatggaga gtcacatgta ttttatattg gagatagaga gcttgtaaat atacatacag
    32461 gaagagctgg aaaaccagat agattacttt atccatcaaa gtattgtttt caatatgagg
    32521 aagaaataaa atctatatat caaaatataa cacaggcttt tgatataaaa aatggacctc
    32581 tttatgtaca aatgttagtg ggagcatatg gaataaaggt atctgaagta atgccaagat
    32641 tacctggagg ttgtgactat atggctattt ctcaaataac agatttagac ataggaagtt
    32701 tatttgcaaa ctttagtgtg aataatgaag taaattttga tgaaataaag aaatataata
    32761 tgcatttatc aaaatgtgta tatgcattac caatatacat aagacctgga acaataaaaa
    32821 agataaataa tatagataaa atagaaaact tagattatgt cactcaattt ctattattag
    32881 taaaagaagg agatacaata ggattatatg aggatgagag acaggattgt ggaagagttt
    32941 atggaatagc aaataatata tttgatgcaa atataaagaa gaaatgtatt catgaaatga
    33001 tagaaatatt tgatgaaaat aatgaaaata tgatagaaaa tttttaaata agggagtatg
    33061 atagatatga agctaataac tcatgaacaa attatgtcat taaacatttc tcagaaacaa
    33121 ggatatgaat ggataaaaga atctttttta acaaaaaaag attgtcaatt accaccaaaa
    33181 ataagcatgg tagaagaagg tcatgtattt tacaatgtaa tgccgtcctt aaatcataac
    33241 catatgattg gtggaataaa aatggttaca agattgccag ggagaaatcc aagtttaaat
    33301 tcagatatat ttctatacaa tatgaaagat gctaaattaa aggctatttt ggatggtaat
    33361 tatattacag cttttagaac tgctgctgtt gctggtttgg ctattgatac ttttgctaaa
    33421 aaagatttta atactatttc tattatgggt ttaggtgtaa ctgcttctgc tataatgaat
    33481 atgctttcgt atactcttaa agataaaaat ataaatataa agttacttaa atataaagac
    33541 caagcagagt catttataaa aaagtatgaa aaaaatgaac aatttagttt tgaaatatgt
    33601 gatacacatg aagaacttat tgtagattca gatgtagtta tttctactgt gacatataca
    33661 gatgaatatt tatcagaatt taaatggttt aaaaaaggtg ctttattatt acctgtacat
    33721 gttatgggat ttggaaatgt tgattatcta tttgataaag tatatttaga tgattataat
    33781 catttgaaac atctagaaaa tatagataga tataaatatt cttgtgaatt ttataatgtt
    33841 ttaagtaaac aagctaaagg aagagaaaat gatgatgaga gaataatatc ttacaatata
    33901 ggtttgtcaa tacatgatat attatttgca aacaaaattt acgaattgtg tgaaacacta
    33961 aattgtgaag agatagaatt aaaagaacca aaagaaaaat attggatata gtttatttca
    34021 ttcatattta caataattta ttgtaaatat gaatatgaag taatatattg ttagaaaatt
    34081 agcatgaata ggatagataa atttatggat aaatataatt taattaagtt cttatctatt
    34141 ggaaacgaat atataggaaa tttagttgca ttagaatcaa ataaagcaat accttttgat
    34201 ataaagagag tttattacat atatgatgta ccaaacaatg taaagagagg atttcatgca
    34261 cacaaaaact tggaacaagt actagtttgc ctaaatggaa gtgtgaagat aaaatgttat
    34321 gatggtgtaa aagagagtat atttaattta cataaaaatg atgttggatt gtatatagga
    34381 acgggagtgt ggcatgagat gtttgacttt gatgaaaaaa ctgttttaat ttctatagca
    34441 tctgaacatt acaatgaaga agattatatt agagactata aagaattttt aaagtattta
    34501 aataatatgt agttattata cagttagtga tgtattttct taattaaata aatttaagat
    34561 agccatatag agaataaata gattaggatg tgaatttatt gttggttaaa tttaataatt
    34621 tattaaaaca gttccaaact cataaagaag aatatgaaca gaagattaag gatgtaatgg
    34681 aaagtggata ttatatcctt ggaaatgaag taaggcattt tgaagaagaa tttgctagct
    34741 atgtaggtag caaatattgt gttggtttag ccagtggttt agatgcactt ataattgctt
    34801 ttagagcatt aggaataagt gaaggtgatg aagtaatcgt gcaatcaaat acatatattg
    34861 ctagtgttat gggaatcagc ataaatggag caacaccagt atttgtagaa ccaaatgaat
    34921 attataatat agattcatct aagatagaag aaaagataaa tagcaaaaca aaagctatat
    34981 tagtagttca tttatatggt caagcaagta aaatgaaaga tatacaagaa ttatgtgata
    35041 agtataactt gtatttaatt gaagattgtg ctcaatctca tggagctaaa tatgatggaa
    35101 aaatgacagg aacatttggt gatataggtt gttttagttt ttatccaagt aaaaatttgg
    35161 gtgcatttgg agatgcagga gctatagtaa ctagtaatga ggaaatagct gataaagtga
    35221 aaattcttag aaactatggt tcagaaaagc gatattataa taaatttgtc ggatataatt
    35281 caagattgga cgaaattcag gcaggtttat taagagtaaa acttaaatat ctaaatgaaa
    35341 ttgaaattga aaaatcaaaa atcgtagata gatatcttaa tgaaattaat aatcccaaag
    35401 tcatacttcc aaagatagaa aataattgta ctcatgtatg gcacttattt gttttacaag
    35461 ttgaaaaacg agatacattt atggaatact taaagaaaaa tggtatagag tctataatac
    35521 attatccgat accaccacat ctatctgaag catatgctta tttgggatat aaaaagggga
    35581 gttttaatat agctgagaac tattcagata aagttgttag tataccttta tttgtcggta
    35641 tggaagatga agaagttagt tatataatta aaattataaa tgaatattaa actaatcata
    35701 ataatatcaa aaataaattt tctatatatc atttatttaa attt
//
LOCUS       DAELSY010000025        33933 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.025, whole genome shotgun sequence.
ACCESSION   DAELSY010000025 DAELSY010000000
VERSION     DAELSY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.025"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            86..1144
                     /locus_tag="KPG09_001103"
     CDS             86..1144
                     /locus_tag="KPG09_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBG0965726.1"
                     /translation="MIVCNNPGTWMRMYPQLRHAVWHGVTLADFAFPFFVISLGVTIP
                     ISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNPDLNSVRILGVLQRMGLVY
                     FVTSLVYLLLKKLNVGSTATIITFLCIATFIIVGYYILAKPYGFELEGSLAQLVDLHF
                     FKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENIGEYKKFFKILVMSIILLI
                     GAFIFNQYFPFNKRLWSSSFVLLMAGSYGMLLSIFYFICDIKNKSKIFTPIIALGSSP
                     IFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELITPWAGTTWDSLIFSLLYVL
                     FWVIVMSIMYKKKIFIKI"
     gene            1398..3422
                     /locus_tag="KPG09_001104"
     CDS             1398..3422
                     /locus_tag="KPG09_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0965727.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAGT
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3642..4328
                     /locus_tag="KPG09_001105"
     CDS             3642..4328
                     /locus_tag="KPG09_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBG0965728.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4423..5478
                     /locus_tag="KPG09_001106"
     CDS             4423..5478
                     /locus_tag="KPG09_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBG0965729.1"
                     /translation="MIKKISTILSLVLLISISSTIEVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGIKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTAKLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5593..7152
                     /gene="murJ"
                     /locus_tag="KPG09_001107"
     CDS             5593..7152
                     /gene="murJ"
                     /locus_tag="KPG09_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG0965730.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDDKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRRR"
     gene            7194..8900
                     /locus_tag="KPG09_001108"
     CDS             7194..8900
                     /locus_tag="KPG09_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG0965731.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNDTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8922..9995
                     /locus_tag="KPG09_001109"
     CDS             8922..9995
                     /locus_tag="KPG09_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBG0965732.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEYISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10007..10729
                     /locus_tag="KPG09_001110"
     CDS             10007..10729
                     /locus_tag="KPG09_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0965733.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10745..11902
                     /locus_tag="KPG09_001111"
     CDS             10745..11902
                     /locus_tag="KPG09_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBG0965734.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNIDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTIFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11905..12744
                     /locus_tag="KPG09_001112"
     CDS             11905..12744
                     /locus_tag="KPG09_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0965735.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNEDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYTFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12759..13934
                     /locus_tag="KPG09_001113"
     CDS             12759..13934
                     /locus_tag="KPG09_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBG0965736.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13963..14712
                     /locus_tag="KPG09_001114"
     CDS             13963..14712
                     /locus_tag="KPG09_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0965737.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYKRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14734..15642
                     /locus_tag="KPG09_001115"
     CDS             14734..15642
                     /locus_tag="KPG09_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0965738.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15690..16442
                     /locus_tag="KPG09_001116"
     CDS             15690..16442
                     /locus_tag="KPG09_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0965739.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16420..17763
                     /locus_tag="KPG09_001117"
     CDS             16420..17763
                     /locus_tag="KPG09_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBG0965740.1"
                     /translation="MPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLAEVGNENVV
                     CVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVG
                     TPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNNVK
                     VELASNPEFLSQGTAVKDTLYASRIVIGVESEWAREILEEIYKPFNQPIIVTNRNSAE
                     MIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGS
                     CFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGL
                     TFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIE
                     DAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEYYSIGR"
     gene            17769..18860
                     /locus_tag="KPG09_001118"
     CDS             17769..18860
                     /locus_tag="KPG09_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0965741.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18944..20857
                     /locus_tag="KPG09_001119"
     CDS             18944..20857
                     /locus_tag="KPG09_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG0965742.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNSELKKVAVAIIDDDKDKIGRIIHNVKIVGTTSDIKAVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21056..21763
                     /locus_tag="KPG09_001120"
     CDS             21056..21763
                     /locus_tag="KPG09_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0965743.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYRWGATGPNAFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            22065..24176
                     /locus_tag="KPG09_001121"
     CDS             22065..24176
                     /locus_tag="KPG09_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBG0965744.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDNWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTAL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24374..25339)
                     /locus_tag="KPG09_001122"
     CDS             complement(24374..25339)
                     /locus_tag="KPG09_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG0965745.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25624..26580)
                     /locus_tag="KPG09_001123"
     CDS             complement(25624..26580)
                     /locus_tag="KPG09_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG0965746.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVDIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26948..27736
                     /locus_tag="KPG09_001124"
     CDS             26948..27736
                     /locus_tag="KPG09_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0965747.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVDDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(27859..28329)
                     /locus_tag="KPG09_001125"
     CDS             complement(27859..28329)
                     /locus_tag="KPG09_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBG0965748.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            28468..29112
                     /gene="uppS"
                     /locus_tag="KPG09_001126"
     CDS             28468..29112
                     /gene="uppS"
                     /locus_tag="KPG09_001126"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBG0965749.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29155..30060
                     /locus_tag="KPG09_001127"
     CDS             29155..30060
                     /locus_tag="KPG09_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0965750.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTSQQQKVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            30375..31922
                     /locus_tag="KPG09_001128"
     CDS             30375..31922
                     /locus_tag="KPG09_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0965751.1"
                     /translation="MNKNSMTKIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIMILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIINLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            31925..32323
                     /locus_tag="KPG09_001129"
     CDS             31925..32323
                     /locus_tag="KPG09_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG0965752.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            32363..>33933
                     /locus_tag="KPG09_001130"
     CDS             32363..>33933
                     /locus_tag="KPG09_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBG0965753.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGVGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKKILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPN"
BASE COUNT        13535 a         3603 c         5977 g        10818 t
ORIGIN      
        1 aatttcaaat aatgttgtag attctaaact tacgaatagt aggattaaat caatagatat
       61 aataagaggg ctatcaatag ccctcatgat agtctgcaat aatcctggga cttggatgag
      121 aatgtatcct caattaagac atgctgtatg gcatggtgta acacttgcgg attttgcatt
      181 tccttttttt gttatatctt taggtgttac aattccaata tcaataaata gtaaactaaa
      241 aaataataaa tccacactga gtataatact tagtatattc aagagaagta tcttattgat
      301 attatttgga tttttcttaa attacctagg aaatcctgat ttaaattctg tgagaatact
      361 tggagtactt caacgtatgg gattagtcta ttttgtgact agtttagtat atttactact
      421 aaaaaaatta aatgttggaa gtactgctac aataattact tttctatgta tagctacatt
      481 tataatagtt gggtactaca tattagcaaa accttatgga tttgagctag aaggttctct
      541 tgcacagtta gtggatttac atttcttcaa gggacatttg tacaagccag aatttgaacc
      601 agatggtttt ttaactagta tagtagcaat ttcttcaggt atgttgggat gcactatggg
      661 atgtgtactt ttaaaagaaa acataggaga atataaaaaa ttctttaaaa tactggttat
      721 gagcataatc ctgctaatag gtgcttttat ctttaatcag tattttccat ttaataaaag
      781 attatggtct agctcatttg tacttttaat ggctggcagt tatggtatgt tactgtcaat
      841 attttatttt atatgtgaca tcaaaaataa aagtaaaata tttaccccaa taatagcact
      901 tggaagtagc cctattttta catatatgtg ccttgagata ttgagtcatg ttttttggaa
      961 tgttccaaaa cttacgaaca aagtcgatta tccaactacc ttggtagagt ggactacata
     1021 tgaacttata acaccttggg caggtacaac ttgggattct cttatatttt ctttattgta
     1081 tgtcctattc tgggttatag taatgtctat aatgtacaag aaaaaaatat ttattaaaat
     1141 ttagtgcaat atttttagat gataaagtct ttgatttgtt caaatatacg gaaactatca
     1201 aatataatca aaatttctcc tcgataaaat ttaaaaacca tgccataaaa gattacaaaa
     1261 agtttacaat ttgtaatttt ttgtgctata atgaaaaaag atttacataa tattgaattt
     1321 atgtaatata aaacaagctt aagatgtatt aaaaaagatt taaagattta aagaaccaaa
     1381 aaattagggg ggatatgatg aaaaaaacaa caaaattatt ggcaacagga atgttgtctg
     1441 tagcaatggt ggcaccaaac gtagctttag cggctgaaaa tactactgca aatacagaat
     1501 ctaattcaga tattaatata aatttacaaa gaaaaagtgt agtgttagga agtaaatcta
     1561 atgcaagtgt aaaatttaaa gaaaaattaa atgctgattc aataacatta aattttatgt
     1621 gttatgatat gccacttgaa gctactttaa attacaatga aaagacagat tcatatgaag
     1681 gtgtaataaa ctataacaaa gacccagaat acttaaatgt atgggaactt caaagcataa
     1741 aaataaatgg taaagatgaa cagaaagttt taaacaaaga agacttagag tctatgggat
     1801 taaatcttaa ggattatgat gttacacaag agtttataat aagtgatgca aattcaacaa
     1861 aagcagtaaa tgaatatatg agaaaaactt ctgcgccagt aaaaaaactt gctggagcta
     1921 ctagatttga aactgctgtt gagataagta aacaaggttg gaaagatggg tctagtaagg
     1981 ttgtaatagt taatggagaa ttagcagcag atggaattac agctactcca cttgcatcta
     2041 catatgatgc accaatatta cttgcaaata aagatgatat accagaaagt acaaaagcag
     2101 aattaaaacg tctaaatcca agtgatgtaa taattatagg tgatgatggt tcagtttcac
     2161 aaaaggcagt atctcaaatt aaatctgctg taaatgtaaa tgtaactcgt attggtggag
     2221 ttgatagaca tgaaacatct ttacttatag caaaagaaat agataagtat catgatgtta
     2281 ataaaatata catagcaaat ggatatgctg gtgaatatga tgctcttaat atttcatcaa
     2341 aagctggtga agaccaacaa cctataatat tagctaataa agactctgta ccacaaggta
     2401 catataattg gttatcttca caaggtttag aagaagctta ttacataggt ggtagtcaaa
     2461 gtttatcatc aaaaattata gaccaaatat ctaaaatagc aaaaaatggt acttcaaaaa
     2521 atagagtttc tggagcagat agacatgaaa caaatgcaaa tgtaattaag actttctatc
     2581 cagataaaga gttgagtgca atgttagttg caaaatctga cataatagtt gactcaataa
     2641 cagcaggacc tttagcagct aaattaaaag ctccaatact tataactcca aagacttatg
     2701 tttctgcata ccatagcaca aacttatcag aaaaaacagc aggaacagta tatcaaattg
     2761 gtgatgggat gaaagatagt gttataaatt ctatagcatc tagtttatca aaacacaatg
     2821 caccaacaga accagataat tcgggttcag cagcaggaaa aactgtagtg atagacccag
     2881 gtcatggggg ttctgattca ggggcaacaa gtggattaaa tggtggagca caagaaaaga
     2941 aatatactct taatacagca ttagcaacta ctgaatattt acgttcaaaa ggtataaatg
     3001 ttgttatgac tagagataca gacaaaacaa tggctttagg agaaagaaca gcattatcaa
     3061 acactataaa gccagattta tttacaagta tacattacaa tgcgtctaat ggagctggta
     3121 atggtgtgga aatttattac aaagtaaaag ataaaaatgg tggaacaact aagactgcag
     3181 catcaaatat cttaaaaaga atactagaaa aatttaacat gaaaaataga ggaatcaaga
     3241 caagaacact tgataatgga aaagattacc tatatgtgtt aagaaataac aattatccag
     3301 caatacttgt tgaatgtgca tttattgaca ataagagcga tatggataag ttaaatactg
     3361 cagaaaaagt aaaaacaatg ggaactcaaa ttggaatagg aatagaagat acagtaaaat
     3421 aaaataaaaa atattaaagc aaaacatatc atctcctgtt ataatgtaaa atataacaag
     3481 agatgatata tttttgtaaa gaatcaaatt gtttacaatg atgtttcttg tattgataat
     3541 ctgctcatta ttgagtataa tatagaaata agtcaaaaat aagatagaaa taagttgaaa
     3601 ttaagaatta aaaaaatttt tattccaata tggaggtaac tatgtctgga tatactaatg
     3661 atgagtgtga aatacctaaa attaaaagct atccaggggc agataaagaa attgcaagtg
     3721 agattgacta tagcatagta aaaggaacgg ttttattcga cttgtaccaa agaattatgg
     3781 atttagtttt atctataata ggacttgtga taggtttacc tttaatagca atatttggaa
     3841 ttcttattaa aatagaggac aaaggaccaa taacttataa gcaagaaaga ctaggaaaat
     3901 gcgggagaag attctatata tataaattaa gatctatgag aacagacgca gaaaaatttg
     3961 gcgcacaatg ggctgaaaaa gatgatccta gaataactaa ggttggaaag tttattagga
     4021 aaactagaat tgatgagatt ccacagctgt ttaatatatt aaagggggat atgggcttaa
     4081 taggccctag accagagaga ccgaacttta ctgttcaatt caatgaggaa atacctggat
     4141 ttatcaacag gctcgctata aaacctgggc ttacaggttg ggcacaagta aatggtggat
     4201 acgaaatcac tcctgaagaa aaacttaaag aagatattta ctatataaaa aataggagta
     4261 tattacttga ctttaagata ctctttaaaa ctgttaaagt agtattgaca ggagacggag
     4321 ctagataatt ttaaaatatt agattactaa tcaatgaaaa agctctttaa aaagagcttt
     4381 tttattgtca aaaaatacaa aaaagaaaag gaatgagaac ttatgattaa aaaaatttct
     4441 actatattat cgttagtttt gttaatctct atttctagca caatagaggt atttgcagat
     4501 gcaaatccca aaagagaatt gatagaaggg agtataccag agatttcgac agaattaaat
     4561 aaaagagcat ttaaggattc taaagaagta attcttgtaa atgaagagtc aattgtagat
     4621 agtataagtg caacaccttt agcatatgca aaaaatgctc caatagttgt aactaagagt
     4681 aaaaatcttg gtagagtaac aagaaattat ctaaaagaac taggtccaga gaaggttacg
     4741 atagtaggtg gtttaaaggc agtatctaaa gatgctgaac gtaatataga aaaaatggga
     4801 ataaaagtag aaagaataag aggaaaagac agatatgata catctttaaa gatagctaga
     4861 gaaatgtata gaacagtagg atttgatgaa gcatttttac ttagctcaac aacaggatta
     4921 gagaatgcca tatctgtata ttcttatgcc gctaaaagtg gaatgccaat aatatgggct
     4981 aaagatgaag ggtttgagga acaaattgat ttcttaaaag gtaagaatct taaaaaaata
     5041 tatgcattag gtgattcaaa agaatttatt gcagagattg attctaactt gaaaaatata
     5101 gaaggaataa agcaaataaa taagtcaagt acaaatgttg atttaataaa gaagttttat
     5161 gatgaaaaag atataaagaa aatctatact gctaaattag actttggtag cagatctgat
     5221 gtaaacgaat atatttcttt gggagtagta tcagcaaaag aaaatatgcc aatactgatt
     5281 tgtagtgata acttaagtcg tgcacaagat aaattcttga aagatagcaa tataaatgat
     5341 gtagttgaag ttgggtatac agtaggtgat tatagtctat ttaagagcat atttaatcta
     5401 acattcttat cttgtatagt attaatacta ttattactat tgattacatt tagggcactt
     5461 agatatgagt cgaaatagtt tttaatttat aaggaagctt ttcataggtt aaatttactt
     5521 aattagaata atgtttataa gcagttttaa aagttctact tttagagttt aaattagata
     5581 aggagaaaga agatgtcaaa aacagctaaa gcagcattgt ggattatggc agccactatg
     5641 ttttcaaaag tattaggttt ccttagagaa cttgtacttg caaatttcta tgggacaggt
     5701 atgtatgcag acgtatttgt attaacatta aatatacctg gtttaatcat agcagtcata
     5761 ggctctgcag ttgctacaac ttatatacct atgtactttg agacaaagaa aagactcgga
     5821 gatgaaggtg ccttaaaatt tactaataat gttttaaata tatgctatat aatggctata
     5881 gtaatagcta ttattgggct tttatttaca gagcaatttg ttacagtatt tgcagcagga
     5941 tttagaaacg accctgctaa gttccaagca gcaatattat ttactaaaat aatgatttca
     6001 ggagttttat tcctaagtgg aagtaagata tttagttcgt ttttacaggt gaatgacagt
     6061 tttgtaattc ctgggcttat aggaatccca tataatatta taataatagc agcaatagca
     6121 ctaagtgcag gtaaaaacgt atggatacta ccagcaggag cactattggc tatggcgagt
     6181 caactattat ttcaactgcc atttgcattt aagaagtcct acaagtataa gccatatata
     6241 aacttaaagg atgagtctat aaaagaatta gtaaacctag tattgcctat gttggtagga
     6301 gtagctgttg gacagttaaa tatatttgtt gacagattat tggcaactac tttgggtgat
     6361 ggaaagttgt ctgctcttaa ctatgcaaat aggctaaatg agtttgttat ggcattattt
     6421 gttacatcta taataacagt tatatatcct aagctagcaa aaatgtctgg taaagatgat
     6481 aaagaaggat ttataagtac tattgtaaaa tcttcaaatt gtattatatt ggtggtgctt
     6541 cctatatcta taggagctat tatattagca gagcctcttg ttaggatatt atttcaaagg
     6601 ggtaaatttg atgcgttatc aacagatctt acatctatag cgcttagatt atattcacta
     6661 ggtttattgg cttgtggagt aagagatgtc ttatataggg cattttactc tctttcagat
     6721 acaaaaacgc caatgataaa tggaagtata gctttaataa taaatatagt tcttaattta
     6781 atattgataa gaccattagg tcatgcaggt attgctatat caactagtac atccaatata
     6841 attacagtta tattgttatt tatatctctt aaaaagaaaa atggatattt tggaggggat
     6901 aaaattataa agactggtct aaaaagtttg gtagcatctg gtgtgatggc agtagctact
     6961 ttattgatat ataataatct atatgcattt atgggttcag gaacaattaa agaaattata
     7021 tcagttggag ctggagtatt aggtggagct tctgtatata ctgtattgat agttttattt
     7081 aaggtagaag agatggactt agcttttgaa tttttaagaa aaggaaaaca aaaactactt
     7141 agaagaagat aattaccttg aaattttaaa ttagagtcag aaaggatgaa aatatggatt
     7201 ataagaataa ttatgagatg tggttaaatt ctccttattt tgatgagcaa acaaaaaatg
     7261 agctattaag cataaaagat gatgaaaaag aaatacaaga tagattctat aagaatttag
     7321 agtttggaac aggtggttta agaggtataa taggagcggg aactaataga atcaatattt
     7381 atactgttag acgtgcgaca ctgggcgttt taaattatat catgaagact caaggagaag
     7441 aaggtaaaca aaagggaata gttatagcac atgatagtag atacatgtct agggagtttt
     7501 gtatagaagt agcaaagact ttaagtgctt atggtgtaaa ggcttatatt tttgaagaat
     7561 taaagcctac accagaactt tcttttgctg ttagatattt aaagtgtgct atgggaatag
     7621 taataacagc aagtcataat cctaaagaat acaatggata taaagtatat gactcagatg
     7681 gtggtcaaat ctgtatagat atggcaaatg atataatagc agaagttaat aagattgatg
     7741 attacagcac tattaaaagt atagattttg aagaagcttt atctaaaaat ttaataacta
     7801 tattagataa tgaagtagat gatgagttta taaaagctgt taagaagcaa gttttaagac
     7861 aaaatataat agatgaatac gggaaaaaat taaaaataat atatacacct attcatggta
     7921 caggaaataa accagtaaga aaagtcttaa atgaatgtgg ttttgaaaat gtaatggtag
     7981 ttaaggagca agaattacca gattctaatt tttcaacagt aaagtatcct aatcctgaag
     8041 aaaagtcagt gtttaatata gctattgaaa tggcaaagaa taatgacact gatttgataa
     8101 taggaacaga ccctgattgt gatagagtag gtatcgttgt aaaagattca agtggtgaat
     8161 atgtagtctt aaatggaaat caagtcggtt cacttttagt tagatatata ttagagagtt
     8221 tagttgaaga aaataaactt cctaaaaaca atcctacaat aataaaaact atagttacat
     8281 cagaactagg tgcaaaaata gcaaaggctt ataatgtaga ttgtttaaat actttaacag
     8341 gatttaaatt tataggagaa aaaataaaag cgtttgaaga aagcaatgat agaagtttta
     8401 taatgggata tgaggaaagc tatggttact taattggaac tcatgctaga gataaagatg
     8461 gtgttgtatc ttcacttatg atatgtgaaa tggcagcata ctattcttca aaaggtatga
     8521 acttgtatga agctttgata gatacatata ataaatttgg atactacaaa gaggacttaa
     8581 agtcagtaac tttaaaaggt atagatggaa taaaaaaaat aaaagagatg atgctttact
     8641 ttagaagtgt taaaatagat aatgtggctg atgtaaaagt agataaaatt ttagactata
     8701 aagatggtgt tgatgattta cctaagtcag atgtacttaa atttttactt gaagatggct
     8761 cttggatagc tataagacct tcaggtacag aacctaaaat taaattttac tttggagcaa
     8821 attcagacaa tcaagaagat gtagaattta agttgaacaa tttaatatct tatatattaa
     8881 atgtagtgga ttctatataa aaagctaagg ggagataaat aatgaaagta tacaatgtca
     8941 taatggctgg aggaggagga actcgtttct ggccacttag tagacaagaa gtacctaaac
     9001 agcttataaa tttaagtgga gaagatgctt taataaacga aactataaac agaatagatt
     9061 ctctagcaaa aaaagatgat ttatttatag taaccaatga gaagcaattg gaagctttaa
     9121 aggatatagt aaaagataaa tgtttggata gtaatatatt gccagagcca tgtgccagaa
     9181 atacagcagc agctataggc tttgcagcat ttaacataat gaaaaaatat ggtgatggtg
     9241 ttatgtgtgt atatccagct gaccattata taaaagatga aaaagaattt aaatctattt
     9301 tggaaaaagc tatctatata gcagaaaata atgataaact agtaactatt gggataactc
     9361 ctacatttcc atcaacagga tatggataca taaattttaa tagagaaaat acgatagaag
     9421 atgttgcata tgaggtagtg gagtttgtag aaaaaccaaa ctatgaaatt gctaaagaat
     9481 atgtaaactc taaaaagtat gtatggaata gtggaatgtt cgtatggaag gtatctaaaa
     9541 tattagaaga ttttaagaga tacttgccaa aagtatatga aaaattagaa tatataagta
     9601 aatacttagg aacaaaagaa gaaatggaaa aaataaaaga aatataccca actatacaat
     9661 caatctctat agattatggt attatggaaa gaagtaatga tgttatagta gttcctggtg
     9721 attttggatg gaatgatgtt ggttcttggg attctctagg tgcaatatat ccaactgatg
     9781 atgaaggtaa catcaaaaga ggtgaaaata ttactataga tactaaaaat tcaattatat
     9841 attcagatga caaattaata tcaacaatag gtattagtga tttgatagta gtatctacga
     9901 atgatgcagt tatggtttgt agaaaagata aagctcaaga tgtcaaaaaa atagtagaac
     9961 agttaaaaga agaagataga caagaatata tgtaggaggt gttcatttga ttcctaaaaa
    10021 aatacattat gtatggtttg gtggtccaaa aggaaatata gaaaatatat gtataaattc
    10081 ttggaaagaa aagcttccag aatatgagat agtagagtgg aatgaaaaaa actttgatat
    10141 agagaaagaa ataaaaggaa ataagttttt ggaagaatgt tataaaagaa aactttgggc
    10201 attcatatct gattatacaa gaataaaagt actctatgaa caaggtggag tttatatgga
    10261 tacagatatg caaatattaa aggatataac tcctctttta gaaaataata ggctaatctg
    10321 tgggtatgaa gatgatagag agtatataaa tggtgctata ataggtgtag aaaaaggaca
    10381 tcctttttta aaagacttat tggagtatta tgaaaaagaa gtacttactt cttcattgtt
    10441 tacaatacca aaaataatga ctcatcttat ggaaaagaat tataagaaga ttgacccaaa
    10501 taattatgaa gaaggaatac gtgtttacga caaagaatat ttttatcctt ttggatttaa
    10561 agaagacttt acaccagaat gtataacaga aaacactttt gggatacatt ggtggggaaa
    10621 aagttgggct aaaaagagaa attatttctt agaaagcaaa cacttaacag gtgtaaataa
    10681 gatatggaag tgttgtaaaa tatttgcaag taatacttta cgaagctaaa agggagaaag
    10741 taaaatgaag actttaaatg atgatagaaa tattatgatt agaaaatatc tttatatatt
    10801 tgttttgttt atcatattac ttagaaagta tatgatatca tttattgacc caaatataga
    10861 tatagggatg attaaatcag cgttatttta ttcttcaata ggaatactaa tgctactttt
    10921 tttatttgat aagagaaaaa gtataacaga aatggtacta gttggagttt gtgtcttact
    10981 ttatttatta aatagggaag gagctatatt attaatcgtt ttattagctg tatcagctaa
    11041 gcaaatagat gataaattta tagttaaaaa ttatttaata atatcggcat gttttttaat
    11101 ggtagctata ttattattta atttatttcc ttcattaata tttaatcagg aagtccacta
    11161 tagatatata gaaaaaatag atatgcttgt tacaagaatg gattttggac ttggtaatcc
    11221 aaatagtgta ttttatcata tggttactat atatgcagca tatatattct taagatataa
    11281 agattataat aaatgggata gaataatttt atttggttct gcattttttg tttatcaaac
    11341 cacatatagt agaactggat ttttcactat attagcagga ttaatatttg tagaaataat
    11401 aagatggata gatataaaaa aaataaaagg actgccaatg ctcctaaaga cattaccaat
    11461 aatacttact ctatttagtg taattattgg tacaatattt gataaaagca ctttattaaa
    11521 tagattatta gcaagtagac ctaagttctg gcatgtatac ttggctgaaa aggggaattt
    11581 tctaaaacct tttggaaatt catatagtcc tgctataaaa gcaactaatc cattagatag
    11641 ttcttatgta tatataatta gtgtactagg agtagttgct tgtatattat ttatgtattt
    11701 gatgtataaa ggaatagaaa gctttataga aaaagataaa aaagcatatt tagtagcttt
    11761 atttatattt ttactatact cttttgcaga aaatttatta ttagaagctt catttagttt
    11821 tgcagtagta ttactaataa aagaagtgat attaaatgat aaaagggaaa tagatttgtg
    11881 gaagatgaaa agtaggaggt agtaatgtta atatcattaa taatgccaac attaaataga
    11941 tatgatgata tatacttact tatggatagc ttagaaaatc aaacttacaa aaactttgaa
    12001 cttatagtag tagaccaaaa tgataatagc aaagttaaag aaatagttga taaatacatt
    12061 gataagttag atataaagta tataaaaagc tctaaaaaag gattgagtta taacagaaat
    12121 gtaggcatag ataatgctgt agggcaaatt ataggtttcc ctgatgatga ttgtgtctac
    12181 gaaaatgata cattagaaaa agttataaat ttctttaata aaaatgaaga ttataaaata
    12241 tacagttgca aaactatgga ttcaaataaa gttgatgcat ttaagaaaat gtatgatgga
    12301 acttgtgata tcacaagttc caacgttttg gatactataa catccataac tttttttatt
    12361 gattttgaag gtaaagatta tacaagattt gatgaaaaat tgggtgttgg tggagagttt
    12421 ggtgctggtg aagagataga ttatgtttta aacttattga gtttaggttt taaaggaaaa
    12481 tactttggaa atgatataat ttaccatcct gcaaaaaaac attctaaatc taaagaaaag
    12541 tatcaaaaag attataatta tggaagaggc tttggagcgc tttgcaaaaa ggaaatagtc
    12601 tatagaaaaa attatacgtt tgcaaaagtt atggtatcaa aacttgtaag aaatataggg
    12661 ggtcttatat tgagttccaa tagagattat catagtgcca ctataaaagg aagaattaat
    12721 ggatttagac agtacaagtt ataatgagga tggtgtttat ggacaaggtt aaatttttaa
    12781 taaatatgct tttggctttt tttatatatc catttaataa gcataagttt aatggtagag
    12841 atatctggct tataggtgga cattcagggg atatatacaa tgataattca aagttttttt
    12901 atgagtatat gctaaaagaa cataatgatg tagaaactta ttgggtagta aataaagaca
    12961 gtaaagtatt tgataaaatt ccaggcaaaa aattaataag aggaagtgta gaaaactatc
    13021 tgtattatta caattcaaaa gctatagtat tttcacatgc accatcagca gatatagcac
    13081 catacaattt tgctgtacct gttcttaatt attttcataa aaaaacgata aaagtgtttt
    13141 taaatcatgg gactataagc tttaaaaaaa gaaaacctat gaacaaaaag ttcaaaaata
    13201 ttatagataa cctatataaa agctataata tagttacagc aagttctgaa tttgaaagaa
    13261 atgttatggt aaatgattgg ggaatgttag atgactcagt ttatataatt gggaatgcaa
    13321 gatatgacaa tttaccaaca aatgaagttg cacaaactcg agatatatta tatactccta
    13381 catggagaga ttggataaaa tttagttcag gaaaatttac ggatacagat tattttaaaa
    13441 atataatgaa ttttttaaat gatgacaaat taaataaaat attagatgag aaagatataa
    13501 atgtaaaaat ttatatgcat catcttatgc atgagtttat agatgatata aaagaaaata
    13561 taacaggaaa acgaattgtg tttttagata aaggggtaac tctagcaaat gaaatcagaa
    13621 aatcagctgc aaatataact gattattcaa gtgtagccat agactttttg tatatgaata
    13681 gacctatctt gttttaccaa ttcgatttgg atgaatatat ggaaaaagtt gattcatata
    13741 tagatttaaa aagtgaaatg tttgggtctt tggcttataa taatgatgaa gccgtgaata
    13801 aacttattga tattattgaa aataattttg aggttatgga taaccaaaaa aatgaaagaa
    13861 ataagttttt cagatataat gataataaga actgcaagag gatatatgat tgtgtattaa
    13921 gtaaaattaa ataaatattt tataaatgat gaggaaggta atatgaagaa gaatttagta
    13981 tctataatta ctcccatgta taattctgaa aaatttattg aagcaaccat aaaatcagta
    14041 ttaaaccaaa cttatcaaga atgggaaatg ttaattattg atgattgctc aacagataat
    14101 agtcctaata tagtcaaatc ttatatgcaa caggatagta gaataaaatg tataaagact
    14161 gagactaata agggtgtctc taatgctaga aatttagcac taagtaaggc aacaggacaa
    14221 tttatagctt ttttagatag tgatgaccaa tggaatagca gtaagttaga aaaacaagta
    14281 aattttatgt tagaaaatga ctatgtaatt tcatttactt catatgaact gatggatgaa
    14341 aatgataaaa aattaaacaa agtaataaaa gttccaccta atgtagatta taaaagatta
    14401 ttaaaaggaa atatcttagg atgtcttact gttgtgattg ataagtctaa attggatttt
    14461 gaaataagaa tgagtggtgt aagacatgaa gactatgttt tatggttatc gatattgaaa
    14521 aaagggcata ttgctcatgg aataaatgaa gttctagcat tatatagaaa atctagcaat
    14581 tccctaagtg gtaataaaat aaaagctgct atgtggactt ggaatatata tagaaatata
    14641 gaaaaaatac ctttatacaa agctatatac tactttatta attatggaat aaacggaatc
    14701 aaaaaaagtt agacagagag gaagatttaa ttaatgtata aatataaatt tactgtattt
    14761 actccgacgt ataacagagc tcatttactt gaaaatttgt acaatgattt aaaagctcaa
    14821 acttatgatt tcaatgattt tgaatggttg attgttgatg atggaagtag cgacagtact
    14881 aaagagttag ttgaaaaatt tatatcagaa aataaattaa atataagata tatatacaaa
    14941 gaaaatggag gaaaacatac tgcaataaat ctaggtgtaa aaaatgcaga tggagagcta
    15001 tttttcattg tagatagtga tgatggttta attaaagatt ctcttgaaat tgctaatgat
    15061 gagtgggatt ctttagagaa taaagaagga tttagtggag ttgttggact ttgtgtatat
    15121 ccaagtggag atttgatagg gacagaaatg cctgaagata agaaaatatg tcactttgca
    15181 gacctatatt ttaagtatgg cgttaaggga gataaaagca tagtatttgt aactgatgag
    15241 cttataaaat ttccttttcc agaaagaaaa gaagtgagat ttttaccaga aagtgtagta
    15301 tggaatgaaa tgtctaaata ctataaagtt atatgtgtaa ataaacctat gataataaga
    15361 gattatttgg atgatggttt gactaagaat atattaagta aaaatgcatt aagaggtaga
    15421 gcattagagt ttttatattt aataaatcaa aatacatatc cattaagtag atatccatat
    15481 atgtggataa aaaattacat aaatttagcc agatactcat tgttgtctga tagtcattat
    15541 tttggtgaat taagaaaagt atctgataaa ttgttgtatt tagcattatt cccattagga
    15601 tattacaaat atattggaca aagaaaatta gtatctaagt aaacttgttg gtatagtact
    15661 gctagttgct atataaggag agtaaaaata tgaatgagcc tttagtatcg ataattactc
    15721 ctgtttataa ttcagaagag tttttatcag agacgataaa gtcaatccaa aatcaaactt
    15781 ataaaaattg gcagctttta ttagtggatg attgttctaa ggacaattca agtgaaatta
    15841 taaaaagttt tcgaaaagaa gatgctagaa taaaatatat aaagcttgaa aaaaattctg
    15901 gagctgcagt atctagaaat gttggtatta agaatgcgga aggtcgattt attgcatttg
    15961 tagatagtga tgatttatgg gatagtagaa aattagagat tcagatagaa tatatgttaa
    16021 aagaaaatgt aggtttttct tttacatctt atagatatat gagacaagat gggtcaaaaa
    16081 cgaataaggt tgcaagagct cctaagaaaa ttgactatga aggattactt agaaatacta
    16141 taataggatg ttctacagtg gtcattgata aggagattgt aggagagttc agcatgcctt
    16201 tggtaagaag aggacaagat actgcgacct ggttacagtt acttaaaaag gaaaaatatg
    16261 catatgggat tcaggaggat ttagtgaatt acagattagt tggaaattct atatcaagta
    16321 acaagataaa agcattaaaa cgaacttgga atacttatag aaatgttgaa aatttaagtt
    16381 taccaaaaag tctatatgta ttttgtttct atgtttttaa tgccattaaa aaaagagtat
    16441 aagttttata tacttggagg taaaaaaatg aaaatagcta tagctgggac gggatatgta
    16501 ggtttggtta caggtgtttg tctagcagag gtaggcaatg aaaatgtagt ctgtgttgat
    16561 gtagatgaac aaaaagttaa atcaatgaaa cttggaatag cacctatata tgaagaaggc
    16621 ctagaagaac ttatggttaa aaattacaac agaggaagaa tagattacac tacagactat
    16681 aaaaaagctt atgaagatgt agatgtaata ttaatagctg taggtacacc tgagagaagt
    16741 gatggctcag ccaacctaga ttatataaag gcagttgcaa agcaaatagc tgaaactgtg
    16801 acaaaagata ctctagtagt aataaaatct acagttccaa ttggtacaaa tgaagagata
    16861 gaagattata taaatagaaa cctaaaaaat aatgtaaaag tagagcttgc atctaatcca
    16921 gagtttttat cacaaggaac agcagtaaaa gatactctat atgcttcaag aatcgttata
    16981 ggtgtagaaa gtgaatgggc tagagaaata ttagaagaaa tctataagcc ttttaatcaa
    17041 cctataatag ttacaaatag aaatagtgct gagatgataa aatacgcatc aaataacttc
    17101 ttagctctta aaatatcatt tatgaatgat attgcaaatc tttgtgaaat agcaggagct
    17161 aacatagaag atgtagcttt aggaatgagt tatgatgata gaataggaag taagttttta
    17221 aaggcgggaa tagggtatgg cggttcttgc tttccaaaag acacaaaagc tcttcactgg
    17281 ttatctgaac ataatggtta tgaattaaaa acagtaaaag ctgcaataga agttaatgaa
    17341 agtcaaaaac ttaaattagt tagaaaggca tctaagttag tagagagctt tcaagatatg
    17401 aaagttgctg ttttaggttt gacttttaaa ccagacactg atgatataag agaagcacca
    17461 tctataccaa atataaaata tttgcttgaa agaggggcaa gagttactgt atacgaccca
    17521 attggtgtaa ataatactaa gaaaatattt ggtgactcta tactttatgc tgattctgta
    17581 gatgatgcga tagaagatgc agagatatgt tttataatga cagaatggaa agatgtatta
    17641 aattatgata tacataagta taaggaaaag atgaaaaatc ctttagtcct tgatggaaga
    17701 aattgttata aaattaaaga tatggaagag ttaggaattg agtattattc gataggaagg
    17761 taaatattat gaagactgtt tgttatttag ctgatgggtc aaatcctcat actttaaaat
    17821 ggtgtagttt ttttaaaaat aaaggatatg acattcatgt aatctcttta aatggaggtc
    17881 acatggaagg aataaaaatt tataattttg gttctaatgt agaagaatta aaaaatgaaa
    17941 atatttctaa aaaaacggga tacttaggtt caataaagca gataaagaag ctagtaaatg
    18001 aaataaagcc agatatattg catgcacagt atgcaagtag ttatggattt ataggaagtc
    18061 tacttgggta tcatccatat attatttctg tttggggtac tgatatttat gatttcccta
    18121 ataatggttt tatacaaaaa caaattataa aacataactt tagaaaagca gactatatct
    18181 tttcaaatag tagagatatg gctaaagagg ctaataaata tacagctaaa catgtagatg
    18241 taaccttttt tggagtagat atggataggt ttaagcctat ggaagtagaa aaagaagacg
    18301 cttttgtaat aggaattata aagagtttag aaaaaaaata tggaatagaa tatttaatac
    18361 aagcatttaa gatgcttaaa gatgaatata aagataagaa aataattctt aaaataggtg
    18421 gctctggaag tcagatggac aacctaataa atttagctaa agaattgggt atagaaaatg
    18481 atgttcagtt tttaggaaga atatctccag aaaatgtaag caagactttt aattcttttg
    18541 atgtaactgt atttccttct ttaagagagg gatttggagt ggcagctata gaatcagaag
    18601 catgtgaagt acctgttata gttacaaatg taggaggaca tccagaatct gtatgggaaa
    18661 atgaaactgg cttaattgta gaaccaaaac aaccagaaga aataaaaaat gctataataa
    18721 aactaatgga aaatgatgaa cttagactta atatgggtaa gaaagggcgt caatttgtta
    18781 gagaaaatta tgaggttaat ttaaacttta atgatataga aaaaatttat gattctatat
    18841 ttgataaata taaaaaataa aatgaaaaaa tacagcaaaa gatagatgtt gttggctatg
    18901 cttaggtgcg tagcttttgc tgtttaaagg agggcttcta gttatgcgag aaagtataga
    18961 tttagagaaa gaaagctttt ttaataggct ttttaaaaaa tataaattta gacatttaac
    19021 actattaatt ctagatattt gtagcgtact agtagcgttt cgaatgtctt taagcttgac
    19081 aggtaattta aatgcattta ccactaatga tataataatt tcagtgtgta tatatatagt
    19141 acttcatata gtatcattta gattgtttaa gtgctacaac acattatgga gatatgcagg
    19201 agaagaagaa attatatcta tttttgtggc aactttagct tatttaattc cgatttatat
    19261 aataaataaa ttattagggt ttgattatcc tataatgttt tatgtattaa atacaatatt
    19321 tattataatg tttacaagtg gcgcaagaat agcttataga gctataagaa tagtcatgaa
    19381 caagacatat tctagaggaa aggtttcaaa tatccttata attggtgcag gagatgcagg
    19441 agaaatggtt atacaggaac taaaaagaaa ttcagaactt aaaaaagtag cagtggctat
    19501 aattgatgat gataaagata aaattggaag aataatccat aatgtgaaaa ttgtaggaac
    19561 aacttcggat ataaaagctg tggtagaaaa gtacaatgta gatgagataa tcttttcaat
    19621 agcaaacata gagaaacgta gaaaaaaaga gattattgat atatgtaaaa acactaactg
    19681 taaaataaag acaataccag gaatttacga aattatagat ggaaaagttg atataaaaca
    19741 gattagagaa gtagagatag aagacttgtt gggtagagag cctataaaaa caaatttaag
    19801 agagataagt aattacattg agggaaaagt aatcctaata actggaggag gaggctcaat
    19861 tggttcagag ctttgcagac agattgcagg atttaatcct aaagagctta ttatagttga
    19921 taattatgaa aataatgctt actcaataca acaagaatta cttagaaaat ataaaaataa
    19981 attagattta aagacagtaa tagcatcaat aagagaagaa aaaagaatgg atgaaatatt
    20041 taataaatat aaaccagaag tggtatttca tgcagcagca cataaacatg ttcctcttat
    20101 ggaatcgagc cctggcgaag ctataaagaa taatatattt ggaactttaa acatagctgg
    20161 cttgtcttca aaatatagag ctaagaaatt tgtccttata tctacagata aggctgtaaa
    20221 ccctactaac ataatgggag ctactaaaag agctgctgaa atgattatac agactatgaa
    20281 tgctgaaagc cagactgaat ttgttgctgt tagatttgga aatgtgcttg gtagtaatgg
    20341 tagtgtaatt ccacttttta agaagcaaat tgaagatggc ggaccagtga cagttactca
    20401 tccagatatc attagatatt ttatgacaat accagaagca gtaggcttag ttatacaagc
    20461 tggggctatg gctaaaggtg gagaaatatt tgtgcttgat atgggagaac ctgtaagaat
    20521 attggattta gcaaaaaact taattaaatt cagtggattt gagccagatg ttgatataaa
    20581 aattgaattt tctggtctta gaccaggaga aaagttgtat gaagaactac ttatgtcaga
    20641 agaaggtctg ttggatacag agcataaaaa gatttttatt ggcagaccaa tagatgttga
    20701 tagagaaaaa ataacaaaat acttaaaact tttaagagaa attactaaca atgaagaggt
    20761 tgaaaaaata gatggtatta tgagggaatt agtgccaact tatattaaac cagaagatgc
    20821 taatatcaaa gaaattgcaa caactagaga aaagtagaat atatttctaa tttgtaacca
    20881 gggggagcaa aaagaaacaa atatatacta aaaagaaaca aaagattaca aaaagattac
    20941 aaaaagatta caaaaaaggt tgcaatttat tttgatgtaa gatagaatgt taattaggaa
    21001 ttggaaacga aggaacaaaa tgttaaattt tcgaaaggat taggtatttg agattatgat
    21061 gaatattaaa aataaaaaac atatattgaa aaaatttata gctatggtgt taatagcagg
    21121 agttgtcaca gtagaagcag gagcaataac ggctagtgca gcagagccaa ctaattcacc
    21181 tatgagtgca acagtagacc agtgtgattt tttaaatgta agaagtggag ctagtgcaaa
    21241 tgatgcagta gtaggaaaga taaacacagg agacaaagtt gaagttttag aattacattc
    21301 aaatggatgg attaaaataa aaagtgttga caatgttaca ggatgggtaa atggagatta
    21361 tttaactatt caaggtggaa atgtagatgc aaaggtacaa aatgttttaa acttagcatt
    21421 taagcaacaa ggtaagccat atagatgggg agcaactgga ccaaatgctt ttgattgttc
    21481 tgggttcact tcttatgtct ataaaaatgg agcaggggtt aatttaccaa gagtatcaag
    21541 atcacaagca acagtaggaa agaaagtatc aagagcagaa ttaaaaccag gagatttagt
    21601 tttctttggt tcaggtggtt caataaatca tgttggattg tatgtaggag attctaaatt
    21661 catacattca ccacaaacag gagatgtagt aaaagttact agcatggctc caggaactaa
    21721 ttatgcaaga agacttataa ctgctacacg tgttttacaa taataataga tattgattta
    21781 cataggattg aatattaaca ttaaatatat aattacattt aatgttttac atagagattt
    21841 aagagtttag taatattaaa atagtgatga acgttataaa aacagcaaag aagataaatt
    21901 tctttgctgt tttttttata ataaattatt tcatattata atcaaatatc aaatataaat
    21961 tttaactata ttattcaata tttgacagat aatatgaatt ttagatatac ttaataagaa
    22021 gtatattgta gaaaaaataa atttaaaaaa ttaaggggga tgaaatgaaa aaaataagta
    22081 tattagtatt atcattgata atgacactta ctatgtgtag tgtaagttca tttgccgatt
    22141 catcaaatga taaggaaatg agagctgctt ggatatctac agtttataat ttagactggc
    22201 ctaagactaa gaataatgaa gccaagcaaa aaaaggaata cacagattta ttggacaaac
    22261 taaaaagtgt aggaataaat acagcagtgg ttcaagtaag accaaaatca gatgctttat
    22321 ataaatcaaa tataaaccca tggtctgaat atcttaccgg tacacaaggt aaagaccctg
    22381 gatatgaccc attaccattt ttaatagaag aagcacataa aagaggaatg gaatttcatg
    22441 catggtttaa tccatataga ataactatgg cagatgagag catagataaa ttgccagcaa
    22501 accatccagc aaagaaaaat ccaagctggg tagttaaaca tggaaataag tattactacg
    22561 acccaggttt accagaagtt agaaagtaca tagtggatag tatagcagaa gtagttcaaa
    22621 attacgatat agatggagtt cattttgatg attacttcta tccaggagta agtttcaatg
    22681 atactgcaac ataccaaaaa tatggaaaag gacaaaataa ggataattgg agaagagaaa
    22741 atgtaaatac tctacttaga gatgtaaaag cgtctataaa gagtataaag cctaatgttg
    22801 tatttggtgt aagcccagca ggtatatgga gaaataagag tagtgaccca acaggttcag
    22861 atacatcagg taatgaaagt tatgtaggta catatgcaga tacaagagca tggataaaac
    22921 aaggattgat tgactatgta gttccacaac tttactggcc aattggtctt aaagcagcag
    22981 attattctaa attagtggca tggtgggcta atgaagtaaa aggaacaaat gtagacttat
    23041 acataggaca aggtatatat aagcaaggtc aatctagtta tggtggacaa aatatagcaa
    23101 aagaaatagt acaacaagta acattaaata gaaagtatag tgaaataaaa ggtagtatgt
    23161 atttctcagc taaagatata gcaaatagta cttctataca aaaggattta aagagtttat
    23221 attctagtag tgaagaacca gttactccac catctaatgt taaagtggag aaattaagag
    23281 gcgacgaaag atatgacaca gcagtagcaa taagtaaaaa aggttgggct acaaattcag
    23341 atacagtagt attagtaaat gggtactcta tagttgatgg cataacatca acaccacttg
    23401 ctacatcaaa tgatgctcct atactattgg taaataagga taatatacct acatccacta
    23461 agaatgagtt aaaaagactt aatccaagta aagttatttt aattggagga aataactcta
    23521 ttggagataa agttgagagt gagataaaag atacattgtc aaatgtatca ataaatagag
    23581 ttggtggttc agatagatat agtacatctt taatgatagc aaaagaatta gtaaaaacta
    23641 atccagtaga aaaactatat ataacaagtg gtacaggtga agcagattct ctttctatag
    23701 cttcaaaagc aggagaagaa aagcaaccaa tagtactagt atcaaaagat aatgtaagtg
    23761 atgaagttta caattggata tcagatttaa aagtaaaaga tgcttatttt ataggtggta
    23821 atctaagtat atctgattca gtaataaata agctggataa ggtcattaca aatgatgtat
    23881 ctaaaaatag aatagctgga gaaaatagag aagaaacaaa tggaaaagta atacaaaaat
    23941 tctatccaaa tgctgaatat tcatcaatgt ttgtaagtaa aagtaatcaa ttggtagatg
    24001 cattaacatc aggtccttta gcagctaaat taaaatcacc agtagttatg cttggaaata
    24061 gtgtaacatc tgctcagaaa actgctttag agcctaagaa aactgctcta gtatacgaag
    24121 ctggagatgg tataaatcaa aacacattaa acactttctt aaacttagtt aagtaataaa
    24181 aaagttaatt taaaatacag agtattttat aaatgtagat taaaatgaaa attatcatca
    24241 taaattagaa aataaaaaag aactatatgc attttgtaat caagtgtaca cagcaacttg
    24301 aaaataatta tgcgatatag ttctttttta gtaattaatc ttatactttt taataaactt
    24361 tatgttgaat tttttattgt ttaaactcta tgtcattaaa tataaaatcg tgtaatatgt
    24421 ctaaagtatc tgggtcaaat ctaagaaccc atccagcttt tccatatata ccacctgtac
    24481 tatgaatttc atcatcaata ggaaattcaa attgcttaat atttaagtca cccattttta
    24541 aaacttgagc tcctaaagaa agtattgcat taggcttcat gttagtttta acatatggaa
    24601 gaactgcatc taataaacct ggatatttag taacaggtaa agtttccgtt ttcttgatta
    24661 tagcttgtat aatctgtctc tgtctgccat ctctagcaaa ggctgtatcg ttgtgtctga
    24721 tacgtgcaaa agatagtgcc tgatatccat ttagagtctg ctttccagag tttctaatgt
    24781 attgtatgct acctttatca tcacttttat tccatttata agtttcagga ataaatttat
    24841 ttagttctcg tatttctcct ttttcaacag taacctcaac gccaccaata gcatcaatta
    24901 tatacataaa tgattcgaaa ttaacacaag cataattttg aatatcaata ttaaaattct
    24961 cttcaattgt ttgtattaat aaatcagctt gtccataagc ataagcatga gtaagttttt
    25021 gtttaccatg accagggatg tctacatagc tatcacgagc taatgatgta agtttgatat
    25081 cattgtgttt gttatcaata gtaagtatca tcatagcatc tgaccttgaa gaatcttcat
    25141 ttggtctagc atctgttccc ataagtagta tgttaattat cccatcttca tttttatggt
    25201 cattgttatc caataaatca ctacttatgc tcgagtcatg aatagcagat aatttataat
    25261 aaaaatatcc atagacactt atagggaata ttactaccaa gaaagccagt agtataacaa
    25321 atttctttaa ttttgacaaa agtacccctt ctttcttctt tttcctgtat attttttaat
    25381 agcatacact taattatatt aaagtattga gatatattca atacttgttt cagttacaaa
    25441 aaaaagacaa tgatataatt tataagaaaa tttatcgacc tatatgtata gttaaaagta
    25501 tatataatat attttttaaa agtaatttat attttaaaat aaaaacacac cccataaata
    25561 caaagcaatt tgcatttaag aggtatgtat ttatcatcaa attattggtt tgttttggaa
    25621 tttctaatct tcaaccataa tatctttaaa tatgaaatca tgcattgcat ctagttcata
    25681 ttcctcaaaa ggtattacat agcctgcatt acctattttt ctaccatttt cagtagaaag
    25741 aggaaactcc atagatttca aatttaaatt tcctatactt aaaagttctt ttcctaaaga
    25801 tattatttca ttaggattca tattagtttt tacatacgga agtattgtgt taactagatt
    25861 aggatattta gtgactggta aatccttaac accatttatt aaactctgta taacacttct
    25921 ttgtcttcta tctctttcat ctgtactatc attttttctt atacgagaaa atgcaagtgt
    25981 ctgatatcca tttaattttt gctcaccaga atttcttata tatttaagag aacctttatt
    26041 gtcattttta ttccatttat aagtttcagg aataaattta tttaattctc ttatttcact
    26101 cttatcaaca gtaacagtaa ctccacctaa agcatcaact atatccataa aagagaaaaa
    26161 gtcaactacg gcgtagtttt gtatatcaag ttggaagttt tcttctatag tttctattaa
    26221 taagttagct tgtccatata cgtaggcatg tgttaatttt tgttctccac gtcctggtat
    26281 atcaacataa gtatctctat ttaatgaagt tagcttaaga cttttatttt tattatcaat
    26341 agtaagtatc atcatggcat cagaacgaga aacttcttct cctggtcttg catctgtacc
    26401 taacaataat atgtttgtga tattttcttc gtttttatag tcattactac taagcgcctc
    26461 tgtatttata ctatcatctt gtatagtttt taatttagta aaaacatatc catatatact
    26521 aacaggaaat gctataacta aaagggctag aaatataacg aactttttga gtcctgacaa
    26581 taatatcccc tactttctaa attttttaat tacacatagt taattatatt aaaaatttgt
    26641 tacataagca ataaaaaaca caataaatta gcataagttt gtcataatat aattattttt
    26701 atagtatttt atagaagaaa gtgtgtagtt attattgatt ttattggaat ataattgtat
    26761 gaagacttaa atttttataa actgatgtgt atattgaaaa aaatattttc ctgaatgtag
    26821 ttatagcatc ttatgaaagg tgtaaattgt aatttaaaat ttttagtgta taataagaat
    26881 tgaaaattca aaccgataaa aattaataaa aaataattat aaattaataa tttgggggag
    26941 gaaatgtatg ataaaattaa tagcaacaga tttagatgga actttgttag atgaaaagag
    27001 cgaaataaat ccagagtttt ataaagtatt taaaaaatta agagagagag ggataatgtt
    27061 ttctgctgct agtggcaggc agtatcaaaa tcttataaaa aaatttgaag atataaaaga
    27121 tgatatgatg tttatatcag aaaatggaac actagtagta tataaaggaa aagaaatatt
    27181 atcaaatcct ttaaataaag aattagtaaa tgaaatcata gagacaacaa gaagtataaa
    27241 agggaaaaaa attgtaatga gtgggaaaaa atacgcttat atagaaagca aagatgaagc
    27301 gtttatacaa gaggttagta cttattatgc aaagtttaaa attgtagacg atttgacgaa
    27361 agtagaaggc gatatattga agatagctgt atttgacttc aaaggtgcag aacataataa
    27421 taatatctac tttgaaaagt tttctgatag agctcaagta tgtatttctg gagtggagtg
    27481 gcttgattta actgcaaaag gtgcaaataa agggagtgcc ataaaaaaag tacaaaaaat
    27541 gttagatata aaatatgaag aaactatggt atttggtgac cagttaaatg atgtggaaat
    27601 gatgaaaagt gcctatcata gttatgccat ggaaaatgca aatgaacatt tgaaacaaat
    27661 tgctagattt agagcaaaaa gaaatactga aaatggcgtt gtagataaga taaaagaggt
    27721 cataaaaatt ggatagtcat atgtatagat gaaaaagatt attttgaaga aatcaaaaaa
    27781 atatcaatgt aattttgata aataagttta tttatgcgaa aattacattg atatttaata
    27841 aacatcaata atagatattt atttttttat gatattcagt attttgtttt ctatttgagt
    27901 tgcattttca gcagaaatta aataaacata tctacctgct ttctcaatta tagtattatt
    27961 tgctatttca gaattgattt tttctccatg tccttctcca aaaggtgctt gtatcatatc
    28021 ttttttataa ttttctagag ttttatatat tttatctacc tcgtcagttt ttataacgat
    28081 aatatcttct agagataatt tttcagaaga attaattaca aaaccttgaa ttatgtcttc
    28141 ctctacatca ttaaaatagt taaagtcctt tgcatcaaag atttttgatt cttttataag
    28201 taaatcagaa gattttaatg ctttttttat ttttgatgta gaaacgtttt tgtaatcacc
    28261 acttgaagaa caactaacca ttcctaatga taagattatc gaaaaaaaca atattaaaat
    28321 ttttttcatg ataaacctcc aagtttttta gactctgtct taaattagtt tactacataa
    28381 ttatataaaa ataaatgtat ttaatgttac agttttgatt aataatgtaa tattttgata
    28441 ataataccta ttgagaggtg attagaaatg agaataccaa aacatgtagg aattataccg
    28501 gatggaaata gaaggtgggc aagtcaaaat ggtatgacaa aagataaagg atatgaaagt
    28561 ggaatagacc ctggattaga gtcttataga ttatgtagag atttaggctg tgaagaggtg
    28621 actttttatg gatttacaac tgataataca agacgtccat cagtgcagac aaaagctttt
    28681 acaaaagcat gtattgatgc tgcaaatata ttaatagatg aaggtgctgc tttattagtt
    28741 gtaggaaata caaaatctcc aatgtttcca gaagaattat taccatatac taatagaatt
    28801 aaagccaaag aaggtcaaat aaaagcaaat cttctggtaa attatgggtg gtattgggac
    28861 ttaaataata tatcaggagt agaaaatgta aataaaaata atatacagga ttacatacag
    28921 tcatttgatg tatccagact agatttaata atcagatggg gtggaagaag gagattaagt
    28981 ggtttccttc ctgtacaatc aatttatgca gatttttatg ttatagataa ttattggcca
    29041 gattttaaga gtgacgatat aaaggacgca tttagttggt atgatacaca agatgtcact
    29101 ttaggtggat aattaaaaat attctgattt aaggttgtag agtgttttaa attattgaaa
    29161 aaaaaacttc ttgatggtaa aataacacta ataatatgta aaagtgtaaa aatttatacc
    29221 aagaagggtg aagaaatgtc aaaaggcaat aataacaata attcaagaaa caaatctaaa
    29281 aagaccagtc atctgaatag aaaaaagcga aaattaaata agaaaaagtt agcagtgttg
    29341 atttgcttta cagttttatt tttatttatt gcatttaagg ctactcaagg tgtagtagct
    29401 ttggtaaaaa gtatggataa aagtaataaa actagtcaac aacaaaaagt taactcagaa
    29461 caatttgacc ttggtaatga agaggaaaat aaaaagaaaa aatatactgt atttatagac
    29521 ccaggtcatg gaggcaatga taaaggaact gaaagcaaga ccagtaatag atatgaaaaa
    29581 gacctaaatc tacaaattgc aaaaaaatta gcaaataaat tatctaaaca aaaggatatt
    29641 caagtagtag tatccagaac tgatgataca tatatatcat taaaggatag agctatctta
    29701 gcaaataata gttctgctga tgtgcttgtt tctattcatt taaatgcaga gaaaaatggt
    29761 aatacagcaa caggtataga aacttggtat aggaataaag ctacagatgg ttcaaaagaa
    29821 cttgcacaga cagtacaaag tactatagta tcctatgtta aagttagaga taggggaata
    29881 gttgaaaata actttgaagt acttagagaa tcaaatatgc ctgctatatt aatagagtgt
    29941 ggttttttaa caacaccttc agaggaacaa aaaataataa atgaaaaata tcaagaccaa
    30001 cttgcggaag gaattgttca aggtgtactt tcatacctag attctaaagg gaataagtaa
    30061 gttaacttta ctttaatgtg aggataaatt taaaagttta actgtgtaaa ttgtattaac
    30121 tttatttcgt aatgttggaa acattgtttg aatttagaaa ctatgaaatt agaaattatt
    30181 ttatctatat ctaatttcat agtttttttt gcaaaaaatt tatagttata aaatttaaaa
    30241 aaataaattt agtataatgt aatgtagtta tttatatgtg attttaactt gcattgattt
    30301 gctatcaatt gtgctttata attagtatat ataaagttta actgtaagat aatttaaaaa
    30361 ataggggaat ttatatgaat aaaaatagca tgacaaaaat ttacagttta aataaaataa
    30421 tttttattat aattggaatt ttaggaatag ttttacttac aagacatatt gaggtagata
    30481 ttaaagtttt aattttaatt ttattaactt tgtttagtat aaatgtattt atttcatatc
    30541 ttaaaattaa gttgtatgag gataaaatta atttaggatt gataaataat tcagataagt
    30601 ttataaatat tactaaagat gagaacttcc taaaacatgt tcaaaattat attatcagta
    30661 atgaaaaaga aaactgtgta atggcttgtt ttgatttatg tagactcaaa cttataaatg
    30721 atatatatgg ttatgagcta ggggatgaag tgttaaataa tattttaagt aacttaaaaa
    30781 attattttgg aaaagaagct atttatggaa agttaaaaag tgatgttttt gtattaataa
    30841 taaagcttga aaatagggaa cagaagattc cttatttagt aaatcttatc aagaataaaa
    30901 ttatgatatt aagtcagatt gacagtttta aaatggatat aaatattgag gcttctattg
    30961 gaatttataa atttaacaat aatgaaattg atataaaaaa agctatagat aatgctgata
    31021 tggcaaggct taaatcaaaa ggactaaagc acatagagta tgtagaattt gataatgcta
    31081 tggaagaaga aatacaatca attatgaaaa tagagagaga tttattcttg gctattaaaa
    31141 ataaagagtt tgtaattcac tatcaaccta aagttgattc aagtacaggt aacattatag
    31201 gttcagaggc attgataaga tggttgcacc catcattagg catggttgga ccaaataagt
    31261 ttatacctat agctgaaaaa aatggtctga taaacaatat tggtagatgg ctaattcaag
    31321 aagtttttat taccattaac aagtggattt cagaaggtat aaatgtctta ccagtctcaa
    31381 taaatctttc tagagtagaa ctatataaaa atgatttagt tgattttttt aaactgatgt
    31441 ttaatacata taatattcca aaagaactta tagaattaga aattacagaa acgacagcgc
    31501 taagagatgt aaaatttata agtgaaaggc tttatgagat taaatcttta ggtatgaata
    31561 tatcattaga tgattttgga gtaggtaatt caaattttat caatcttaag gggataccac
    31621 tagatattat aaaaattgat aggtcactta tattggatat agttacaaat acaaaaacaa
    31681 agtttatggt gaaagcaatt attaaccttt cacatgattt aaacgtaact gttatatgtg
    31741 aaggggtaga ggatatgcat caggtcaaag tcttaagtga actgggatgt aatttcatac
    31801 aaggttatgt attttttaaa cctttggatg agatgaatta taaaaaactt cttactgacg
    31861 gaagttatat aaatctgaaa gatactcttt tagataaatg catgacagtt gaggaggagt
    31921 aaggatgatt tcagaagatg tagattattc aataagaatc ataagagcta tatttaataa
    31981 tggagaagat aaaccaatag atgctaaaat gatattaaaa agtgaagata taccaatact
    32041 aacactatac agactgctta gaaagctttg tgcaaacaat attcttactt ttaacaaaga
    32101 agatggcaag acatacatgt taaatgtgtc attggataca ataagtctaa gaactattgt
    32161 agaagtaatt gatggaaaaa tatatataaa taaatgtacg agatatgtat ctaattgcag
    32221 taattgctta aaaggttgta aagtaaatcg tgaattgcta gaaatagaaa aagaaattta
    32281 taaactttta tcgagaaaat ctataagaca aatactagaa taaaaaattt tcagctatga
    32341 taataattgg aggttttcgg ttatggatga tatatctcaa gataattttc tactgtctaa
    32401 agagtatgag aatagcttag atgtagatac taaaaaagca agtggaatat attatactcc
    32461 taaaataatt gtagattata ttgtaaaaaa aactttaaaa aatcacgata taattaaaaa
    32521 tccatatcca cgaatacttg atatttcctg tgggtgtgga aattttcttt tagaagtata
    32581 tgatatttta tatgacttat ttgaggaaaa tatatatgaa ttgaaaaaga agtatgatga
    32641 aaattattgg actgttgata acatacaccg tcatatatta aactattgta tatatggtgc
    32701 tgatattgat gaaaaagcta taagtatatt aaaagatagc ttaacaaata aaaaggtagt
    32761 aaatgattta gatgaatctg atataaagat taatctattt tgttgtgata gtcttaaaaa
    32821 gaaatggcga tataagtttg attacatagt gggtaatcca ccctatattg gtcataaaaa
    32881 acttgaaaag aaatataaaa aatttctcct agaaaaatat agtgaagttt ataaggataa
    32941 agctgactta tatttttgtt tttataaaaa aattatagat attttaaagc aaggaggggt
    33001 tggcagtgtt ataactccaa gatatttctt agaaagtcta tcaggtaaag atttaagaga
    33061 atatattaaa tcaaatgtca atgttcaaga aatagtggat tttttaggtg caaatatatt
    33121 taaaaacata ggggtatcta gttgtattct aacttttgat aagaaaaaaa caaaagagac
    33181 atatatagat gtatttaaaa tcaaaaatga ggatatatgt ataaataaat ttgaaacatt
    33241 ggaagaactt ctaaagagta gcaaatttga acattttaat ataaatcaga gattgctatc
    33301 tgacgaatgg atacttgtaa ataaagatga tgaaactttt tataataaga tacaagaaaa
    33361 atgtaaatat agcttggaag atatagcaat aagttttcaa ggaataatta cagggtgtga
    33421 taaagcgttt attttaagta aagatgatgt aaagttgaac cttgtagatg ataaattttt
    33481 aaaatgttgg ataaaaagta agaatataaa taaatatata gtagataaaa gtgaatatag
    33541 gctaatatac tcaaatgata tagataatga gaatacaaat aaaaagatac tagatgaaat
    33601 tatagggcta tataaaacaa aattagaaaa cagaagagaa tgtaaaagtg gaataaggaa
    33661 atggtatgaa cttcaatggg gtagagaaaa gcttttcttt gaaagaaaaa agataatgta
    33721 tccttataaa tcaaatgaaa atagatttgc tatagattat gataacaatt tttcaagtgc
    33781 agatgtatat tcttttttta taaaagaaga gtatttagat aaattttcat atgaatatct
    33841 agttggaatt ttaaattcca gtgtttatga taaatacttt aaaataactg ctaaaaaaat
    33901 gagtaaaaat atatatgatt actatcctaa taa
//
LOCUS       DAELSY010000026        32380 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.026, whole genome shotgun sequence.
ACCESSION   DAELSY010000026 DAELSY010000000
VERSION     DAELSY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32380
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.026"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1375
                     /locus_tag="KPG09_001131"
     CDS             <1..1375
                     /locus_tag="KPG09_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0965754.1"
                     /translation="KIEEQDVILNLSKDGIIFTSADGIINTFNKEALKILGINENPQG
                     KNIEDIISEDLNILLEDKEIIDEVVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYI
                     KKLEQNLTNKLREQGQIARYTFKDIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTG
                     KELLSQSIHNASNRSNQPFIAVNCAAVPDSLLESQLFGYEKGSFTGALREGKKGLFEL
                     ANNGTIFLDEIGDMPPLLQTKLLRVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMI
                     REGKFREDLYYRLNVLPIDVPSLRNRKEDIITLMNFFLKNNVRLSPEVITILEKYNWP
                     GNIRELQNVASYVSLMCDSMVFRDDLPPYIRVECANNYEDGFGYTKKEFEEILRILYE
                     RRESSSGVGRGFIIEKLIQEGIEISESKVRKVLSYLSECGYISSKSGRGGSQITSEGI
                     KLYNNIN"
     gene            1723..1968
                     /locus_tag="KPG09_001132"
     CDS             1723..1968
                     /locus_tag="KPG09_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG0965755.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            2003..2728
                     /locus_tag="KPG09_001133"
     CDS             2003..2728
                     /locus_tag="KPG09_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG0965756.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEEENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            2750..4063
                     /locus_tag="KPG09_001134"
     CDS             2750..4063
                     /locus_tag="KPG09_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0965757.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVCGENLKKIV"
     gene            4362..4808
                     /locus_tag="KPG09_001135"
     CDS             4362..4808
                     /locus_tag="KPG09_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0965758.1"
                     /translation="MGGETHFLSFGKDEYKISLEEQENAIESAKASDNSVKLIAFIDD
                     EIVGIATIDSNQKAKGKHVGVLGIVVKEKYWGIGLGKRLMLDLIEWCKSNGITKKITL
                     VTNEENYNAIGLYKKVGFEVESILKKECYYNGVYTDLIGMSLLLGI"
     gene            4897..6882
                     /locus_tag="KPG09_001136"
     CDS             4897..6882
                     /locus_tag="KPG09_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBG0965759.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTITLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIEEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            7106..8074
                     /gene="spoIIIAA"
                     /locus_tag="KPG09_001137"
     CDS             7106..8074
                     /gene="spoIIIAA"
                     /locus_tag="KPG09_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBG0965760.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIDD
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            8064..8585
                     /locus_tag="KPG09_001138"
     CDS             8064..8585
                     /locus_tag="KPG09_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBG0965761.1"
                     /translation="MQIKIIIIALLVGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            8602..8799
                     /gene="spoIIIAC"
                     /locus_tag="KPG09_001139"
     CDS             8602..8799
                     /gene="spoIIIAC"
                     /locus_tag="KPG09_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBG0965762.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            8815..9201
                     /gene="spoIIIAD"
                     /locus_tag="KPG09_001140"
     CDS             8815..9201
                     /gene="spoIIIAD"
                     /locus_tag="KPG09_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBG0965763.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            9286..10491
                     /gene="spoIIIAE"
                     /locus_tag="KPG09_001141"
     CDS             9286..10491
                     /gene="spoIIIAE"
                     /locus_tag="KPG09_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBG0965764.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            10551..11168
                     /locus_tag="KPG09_001142"
     CDS             10551..11168
                     /locus_tag="KPG09_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBG0965765.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            11180..11734
                     /locus_tag="KPG09_001143"
     CDS             11180..11734
                     /locus_tag="KPG09_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBG0965766.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKKQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            11761..12450
                     /locus_tag="KPG09_001144"
     CDS             11761..12450
                     /locus_tag="KPG09_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBG0965767.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDSDSSKTEDSSNVDKQGGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            12560..12922
                     /locus_tag="KPG09_001145"
     CDS             12560..12922
                     /locus_tag="KPG09_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG0965768.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            13007..13513
                     /gene="nusB"
                     /locus_tag="KPG09_001146"
     CDS             13007..13513
                     /gene="nusB"
                     /locus_tag="KPG09_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBG0965769.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            13497..14567
                     /locus_tag="KPG09_001147"
     CDS             13497..14567
                     /locus_tag="KPG09_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBG0965770.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            14564..15769
                     /locus_tag="KPG09_001148"
     CDS             14564..15769
                     /locus_tag="KPG09_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBG0965771.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVMVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            15771..15986
                     /gene="xseB"
                     /locus_tag="KPG09_001149"
     CDS             15771..15986
                     /gene="xseB"
                     /locus_tag="KPG09_001149"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBG0965772.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            15989..16876
                     /locus_tag="KPG09_001150"
     CDS             15989..16876
                     /locus_tag="KPG09_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBG0965773.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            16906..17343
                     /locus_tag="KPG09_001151"
     CDS             16906..17343
                     /locus_tag="KPG09_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBG0965774.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            17377..19242
                     /locus_tag="KPG09_001152"
     CDS             17377..19242
                     /locus_tag="KPG09_001152"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBG0965775.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANNNED
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            19375..20187
                     /locus_tag="KPG09_001153"
     CDS             19375..20187
                     /locus_tag="KPG09_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBG0965776.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEYTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            20207..21886
                     /gene="recN"
                     /locus_tag="KPG09_001154"
     CDS             20207..21886
                     /gene="recN"
                     /locus_tag="KPG09_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBG0965777.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLKSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGSFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            22101..22967
                     /locus_tag="KPG09_001155"
     CDS             22101..22967
                     /locus_tag="KPG09_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965778.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECTIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGDISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKAYDNIRLEKVDIIVNEGKKD
                     TIYKAQLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(23058..23549)
                     /locus_tag="KPG09_001156"
     CDS             complement(23058..23549)
                     /locus_tag="KPG09_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0965779.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIVEAKKIGYTHMRLDTLPSMKSAQGLYEKFGFYDIELYTYNPIEGARYME
                     LKL"
     gene            complement(23610..24050)
                     /locus_tag="KPG09_001157"
     CDS             complement(23610..24050)
                     /locus_tag="KPG09_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0965780.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGCGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFKFNGELDYGGEKVMVLKSN"
     gene            24211..25269
                     /locus_tag="KPG09_001158"
     CDS             24211..25269
                     /locus_tag="KPG09_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBG0965781.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVGYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            25411..26235
                     /gene="spo0A"
                     /locus_tag="KPG09_001159"
     CDS             25411..26235
                     /gene="spo0A"
                     /locus_tag="KPG09_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBG0965782.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            26648..27769
                     /locus_tag="KPG09_001160"
     CDS             26648..27769
                     /locus_tag="KPG09_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965783.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            27787..28662
                     /locus_tag="KPG09_001161"
     CDS             27787..28662
                     /locus_tag="KPG09_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBG0965784.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIVLKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNSLGNTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            28686..29378
                     /locus_tag="KPG09_001162"
     CDS             28686..29378
                     /locus_tag="KPG09_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0965785.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            29386..30210
                     /locus_tag="KPG09_001163"
     CDS             29386..30210
                     /locus_tag="KPG09_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG0965786.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            30215..31282
                     /locus_tag="KPG09_001164"
     CDS             30215..31282
                     /locus_tag="KPG09_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBG0965787.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGKIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            31284..31820
                     /locus_tag="KPG09_001165"
     CDS             31284..31820
                     /locus_tag="KPG09_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG0965788.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            31888..>32380
                     /locus_tag="KPG09_001166"
     CDS             31888..>32380
                     /locus_tag="KPG09_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBG0965789.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQ"
BASE COUNT        13153 a         3221 c         5912 g        10094 t
ORIGIN      
        1 aaaaatagaa gaacaagatg ttatattgaa tctatcaaaa gatggaataa tatttacttc
       61 agcagatggt ataataaaca catttaataa agaagcttta aaaattttag gtataaatga
      121 aaatccacaa ggcaaaaaca tagaagatat catttctgaa gatttaaata ttttacttga
      181 agacaaagaa ataattgatg aagtggtatt tacaaataaa aaatatataa atgtaaataa
      241 aaaaagtatt ttttctatgg ggaataaagc tgggatatat tataacttac aagaaataac
      301 gtatataaaa aaattagaac aaaatcttac taataaatta agagaacaag gtcagatagc
      361 aagatataca tttaaagata taaaaactaa aaataaaaat atggtaaagt gtgtagaatt
      421 ggctaaaaaa atatctaaat cagacctttc tgtattaata ataggagaaa gtggtactgg
      481 taaagaatta ttatcacaat caattcataa tgcttccaat agaagtaatc aaccatttat
      541 agcggttaat tgtgcagcag ttccagatag tctattagaa agtcaactgt ttggatatga
      601 aaaaggctct tttacaggag ctttaagaga aggaaaaaag ggtctatttg aacttgctaa
      661 taatggaact atatttctgg atgaaattgg agatatgcct ccattgctac agacaaagtt
      721 actaagggta ttacaagaaa agcaggttat gcctataggt tcccataatg ttataaacat
      781 agatgttagg gtcatagcag caacaaacaa aaacttacta aagatgatta gagaaggaaa
      841 atttagagag gacctgtatt atagactaaa tgtattgcca atagatgtac catcattgag
      901 gaatagaaaa gaagatataa taacacttat gaatttcttt ttgaaaaaca atgtaagact
      961 ttcgccagaa gttataacta ttttagaaaa atataattgg ccaggaaata taagagagtt
     1021 acaaaatgtt gcttcttatg taagtctaat gtgtgatagt atggttttta gagatgactt
     1081 accaccatat ataagggtag aatgtgctaa caattatgaa gatggatttg gttatacaaa
     1141 aaaagaattt gaggaaatac tgaggattct atatgaaaga agagagtcat cctctggagt
     1201 tggaagaggg tttataatcg aaaagcttat acaggaaggg attgagattt ctgaaagcaa
     1261 agttagaaag gtgctatctt atttaagtga atgtggatat atatcatcta aatctggtag
     1321 aggaggaagt caaataacct cagaggggat aaaactttat aataatataa attaagctaa
     1381 gtatttgata ttgaaatcac atattttgaa tttagatatt taaataataa acttttatat
     1441 ttatagagat taatttatac tatgtcagaa agaatattgt aatttataat taaatattaa
     1501 taggttttta aaataggatt agataggtaa taggttaagt tagaataacc cattacctat
     1561 ttttatttgt aagaaaagtg tacatagagt taaaagtaaa aaatacaact aataaaacta
     1621 ttgaaaacac tagataaata ataaataaaa ataattataa attttggcat atatattgct
     1681 aatcatagag ataagacgac aaaataattg ggaggtttaa tgatgaaata caaaacaaca
     1741 gggacttgtg ctacagaaat tgaatttgaa gtaaaagaaa ataaagttac aaatgtaaac
     1801 tttataggtg gatgtgatgg aaatttaaaa gggctaaaag tattagttga aggcatgaat
     1861 atagaagatg taatacaaaa gttaaaagga atagagtgta aaacaaagcc aacatcttgc
     1921 ccagaccaac tgtctctagc attggaaaat tatatgaata taaagtaata cataaaacaa
     1981 tcgattagtt aggagtttaa ttatgaaacc aataataggg attttaggaa atttaataat
     2041 aatggaaaat ggtatgtttc caggtcttga aaggtcatat gtaaataatg attatataaa
     2101 tgcagtgcta aaaggtggcg gtagtccagt aataattcct gtaaatacag ataaagaagt
     2161 tataaaaaaa cagattgaaa tggttgatgg agtcttaata tctgggggat gggatatcaa
     2221 tcctcaactg tatggagaag aaacaagaga agaaacgaca tttatatatc cagaagtgga
     2281 tgaatttgat ttaattgcaa taagtatagc attagaactt aaaaagccta tattaggtgt
     2341 atgtagggga ctacaaattt taaatgtaag tttaggagga actttatatc aagataataa
     2401 tcttatagaa ggaagttata taaaacatac acaatcttct aaaagacatg tagctactca
     2461 taaagtcgat gttaaagaag gaagtatttt agagggaatt ttaggaaaac aattacttac
     2521 aaatagttat catcatcaaa gtgtcaatca gcttggaaaa ggattaaaag ccatagcata
     2581 ctcaaaagat ggtataatag aagctattga gaaagaggag gaaaattttg tagtaggagt
     2641 acagtggcat ccagaaatga tggtagatta ctgtgataaa atggaaaaac tatttaaata
     2701 ttttataaat atttgttcaa atttataata taccttgggg ggattgaaca tgaacgaaaa
     2761 gaacaaaatg ggattaataa gtattatact tttaggtatt aatgcagtag taggagctgg
     2821 agtttttcta ttaccaggag atgcaatgaa atcatttggt gtagcaagta tatttgtata
     2881 tatatttgat atgttgttag ttttatctat ggcattctgt tttgctgagg ttgcaggtaa
     2941 attcaataaa aatggcgctg cctatgtata taccaaagaa gcttttggag atttttgtgg
     3001 ttttgaagta ggtcttatga aatgggtaat aggatgtatt tcttggggag ctttaatagt
     3061 aggttttcct acatcattat cagcagtatg ggctccagca ggtgaaccac atatacaaaa
     3121 aattataatt gtagccatga tagttgggct tacaattata aacttattag gtgtatctct
     3181 atcaaaaatt gtacaaaatg ttataactgt aggtaaacta attccattaa tactatttat
     3241 aggaataggt atatttttta taaaaggagt taattttact tcttcaacaa tggtacctcc
     3301 aggagctgga gcaacagagt ttggagcagc agcactttta atgttttatt catttacagg
     3361 atttgaatct atagcagttg cagcagaaga tatggaaaat ccacaaaaaa atataccaat
     3421 agcaattata agtgtaatag taattgcatc aataatatat atattaaatc aagttgtatg
     3481 tgttggtata ctaggagatt cgttatctag tacatctaca ccagtggcag atgctgcaag
     3541 aatatgcttt ggaaatatgg gagctggatt ggttaccttt ggaacattgg tttctgttgg
     3601 tggaatttgt atgtgtggtg cttttgtaaa tccaagaagt tgtgtagctt tagctgatga
     3661 taaaatgtta ccaagaatat ttgcaagaaa agataaaaaa ggtacaccat atgtagcgat
     3721 aatagctact atgttaatca ctataccaat agctctatct ggaagttttg ctgaattagc
     3781 agcaataagt gcagttgcta gattcataca atacatacca acatctttat ccgtattagt
     3841 gtttagaaag aaaagaccag aattagtagg tacttttaaa actccttttg gagcagtgat
     3901 tcctctaatt gctgtatgtg ttggaacatg gttgttattc caagcatcta tgcatcaatt
     3961 aattatggga ttaggagctt tggcaattgg agtaccatta tactttataa tgaagtctta
     4021 taatagaaaa gtctgtggtg aaaatttaaa gaaaatagtt taattatgtt ataagaattt
     4081 attgagttaa aattagacat tggcagaaaa ttaaatacaa ataagtatct ataaggctat
     4141 attaaaatat tattaatttt ttattttaat atagccttat attgttatca ataaatgaaa
     4201 aatattgtaa caacagaaga acaatagtat tatgtttata gataaaaaac ttattaggag
     4261 gtaaatttat gccaacaata acattaaaaa atggtgtaga cgtacttatt agagaaggtg
     4321 taagagaaga tgcttaaagc attatagatt tttataatga agtgggtgga gaaactcatt
     4381 ttctgtcatt tggaaaggat gaatataaaa tatctttgga ggaacaagaa aatgctatag
     4441 aatcagcaaa agcatctgat aattctgtta aactgattgc atttatagat gatgaaatag
     4501 ttgggattgc aacgattgat tctaatcaaa aagcaaaagg aaagcatgtt ggagtattag
     4561 gaattgttgt aaaagaaaaa tattggggaa ttggattggg taaaaggctt atgttagacc
     4621 ttatagaatg gtgtaaatca aatggaataa ccaagaagat aactcttgtc actaatgaag
     4681 aaaattataa tgctatagga ttatacaaaa aagttggatt tgaagtagaa agcatactaa
     4741 aaaaagaatg ttattataat ggtgtttata ctgatttaat aggaatgtca cttttattgg
     4801 gaatataatt agtgtacatt ttatctcgaa aaaagtatat taagtatggt ataattaaaa
     4861 aagaatataa cttaatataa ttttggagga cgaattatga aaaatctgtg taaaatatca
     4921 gatgattatt taaacagcaa gcttaagtat ttaaagttat tatcaaaaca atatccaagt
     4981 atatcaaaag caagtacaga aataataaat ttagaggcta tattaaatct tccaaaggga
     5041 acagagcatt ttataacaga tgtacatggt gaatatgaac catttgtaca tgtattaaaa
     5101 aatggttctg gtgtaataaa gagaaaaata gaagaattgt tttcaaatac aataagagac
     5161 agcgaaaaaa aaatgttagc aactcttgta tattatcctg aacaaaagtt agacctaata
     5221 attaagcaag aggaaaatat agatgatttt tatagaataa atatttatag acttatagaa
     5281 ctttgtaaat atgcttctag caaatatact aggtctaaag ttagaaaact gttaccagaa
     5341 aattttaaat acataataga agaacttttg catgagcatg ttaaaagtga acataaagaa
     5401 gagtattata aaagtatagt tgaaacaata gttgacatag gaatagctaa ggagtttata
     5461 attgctattt ctacagttat acaaaaattg gttgtagata gacttcatgt aataggagat
     5521 atttatgata gaggtccaag accagatata attgtcgata agcttattga acatcattgt
     5581 gtggatattc aatggggaaa tcatgatata ttgtggatgg gtgcagcctc aggtgaaaaa
     5641 acttgtatag caaatgcact gaggatatca gctagatatg cgaatttaga cattgtagaa
     5701 gacatatatg gaataaatct gttgccttta gccacttttg ctatagaaat gtataaagat
     5761 gacccttgta aagaatttat tccaaaggtt aatgaccaaa gtgtaacaac aacagaaaag
     5821 tctttaatgg caaaaatgca caaagcaata agtataattc aatttaagct tgaaggtgag
     5881 gttataagac gaagacctga atttgaaatg gaacatagac ttttgcttaa tatgataaac
     5941 tatgatgaag gtacgattac tttaaaaggt aaaacatata aattaaaaga tacttattta
     6001 cctacaatag acaaaaaaga cccatataaa ttaactatgg aagaaagaaa tgttattgat
     6061 aaattagtat cttcatttag aggtagtgaa aaattacaaa aacatgtttc atttttgttt
     6121 tctaaaggaa gtatatattt aaaggctaac tcaaatttac tgattcatgg atgtgtacca
     6181 ttaaatgaag atggaagctt tatgtctatg aatataatgg gaaaagagta taaaggtaag
     6241 gctcttatgg ataagatgga gtctctagca agagaaggat ttttctttaa agataaggca
     6301 gaagaaaaac tatatggtat ggatattatg tggtatttat ggactgggaa atgttcatca
     6361 ttatttggta aagatgatat gactacattt gaaagatact ttatagaaga aaaagagact
     6421 cataaagaaa ataagaatcc atattttaaa cttagagaaa atgagatggc ttgtaaaaga
     6481 ttatttgaag aatttgattt agagcttgat gaatcccata taataaatgg tcatgttcct
     6541 gtggagagta aaaatggaga gagcccaata aaagctaatg gtaaaattct cgttatagat
     6601 ggtggttttt ctagggctta ccaaaaaact actggtatag ctggatacac tttgatatac
     6661 aattctagaa ctttgcaatt agtatcgcat gaaccattta attctgctga agaagccatt
     6721 gcaaatgaaa gtgatatctt atcgacaact gttgtagtag agcataaggc aaagagaaaa
     6781 atggtgagag atactgatga aggagtaaaa atacaagagg aaatagaaga tttaaaacta
     6841 cttttaatgg cttataagaa agggctaata aaagaaatgt agatattata gaggcacttc
     6901 ataattgaag tgtcttcttg ttaatactta ttaaatatat tataaagatt aataatagta
     6961 gtttatatta tacttactaa gtgaatccaa gataaatggg ttcacttttt ttatttataa
     7021 acattattaa aaaaatatag atgttttggt tatattcctt gtcctttata aatagaatta
     7081 tataaacaaa ggaggaggac aagagatgaa taaactttct gatgaaataa taaactctct
     7141 ttctacaact ataagagaaa aggtagaaaa agtctcaaac aataatctga acatagaaga
     7201 aattaggtta cgctcacaaa aaccattgat actaaatgca aactctaaag attattttta
     7261 caatcaaaaa actatgacac ttgatttaaa tcaacaaaac tcatatgtag taacaagaga
     7321 agatgtagag caaacttttc aaataatatg taaatattca atacattcat ttatggatga
     7381 cataaaaaaa ggattcataa ctttgagagg aggtcataga gtagggttag ttggaaaagc
     7441 aatagtagaa gatggacaag tcaaaaatat aaagcatata tcatctttaa atataagggt
     7501 atcaagggaa attattggat gctcggataa gattttaagt catattataa agggaaaaaa
     7561 tcagattaat aatactttga taatatcacc acctcaatgt ggaaaaacaa ctttaataag
     7621 agatatagta agaaatttaa gtaatggtaa tgaagattat ggatttaaag gtttaaaagt
     7681 agctttggta gatgagcgta atgagatagc aggagcttat ctaggtgttc cacaaatgga
     7741 tgtgggaata agaactgata taatagaaac ttgcccaaaa gatttgggta ttacgatgct
     7801 tttaaggtct atgtcaccaa atgttattgt gacagatgaa attgggagtg aaaaagaaat
     7861 aaaggcatta tacacagcat taaatggagg tataggatta ataactactg tgcatggaga
     7921 ttcaatagat gatatacaaa atagaaaaga attaaataga cttctggata aagagttatt
     7981 taaaaaggtt ataattcttt ctgcaaaaag aggagcagga acgattgaaa aaatttatga
     8041 tttagaagaa aagagatggt attttgcaaa ttaaaataat tattatagct ctcttggtag
     8101 gaagtagtta tttgataggt gaacaaatat acaaaactta tactcgtaga cataaacaat
     8161 taaacgattt aataagagtt ttagaaattt tgaggatgga tttatctttc ggactttata
     8221 cattagaaga aatctttaat aggataggtg gaaataaaga gttttgtttt tggaaatttt
     8281 tctatcaaat atcagaagga cttcacaatg aacaaagtaa aactttggaa ataataatat
     8341 cagaaaatat tgatgtctta agtaaagaaa cttatctagg caataaagaa attgaggaac
     8401 tgaaaaatct aatacttact ttagggaaaa gcgatataga gtcacaacaa agaatgatag
     8461 atttatctat agaaaatttg aaaaaacaaa cctatgagac aaaggaagat ataaacaaaa
     8521 aaggcgtatt gtataaaaaa ttagtgacat tcataggtat aggtatatgt attatattga
     8581 tttaatatgg ggaggtttat tatggaaata tcattgatat taaaggttgc aggtgtcggt
     8641 attctaatat cagtattgaa tatgatattg gaaaaaacag atagaaaaga ttgggcaggt
     8701 cttactacat tagcaggtgt aataatcgta ttgggtatgg taataacaga aataagtgac
     8761 ttgtttaata ctgtaagaac aatgtttcaa ctttactaaa aaggagggag taatttggaa
     8821 ataatgcaat taataggaat tgcaatcatt tcaactacac tctgtttagt aattaaaaaa
     8881 gatagaccag agatagcaaa tttcatagca ataataactg gagtaataat actattgtca
     8941 gttatgttta agctaaattt tatagtagat agtatacaag atttggctaa caaggctaac
     9001 ataccgacta tgtatatatc attaataata aaattaattg ggatagcata cttaatggaa
     9061 tttgcgatac aactttgtaa agattgtggg gaaggaaaca ttgcttcaaa actagaattt
     9121 ggaggtaaaa taatagttat gtcaatgtct ttccctatac ttttgtccat tgtagaaatg
     9181 gtggtgaata taattccata gatgtgtatc ttggaatgaa tataagtata ttagagaact
     9241 ttcgttgtat tgaggtttat agagtgaaga gaggcatggt tgcttatgaa aaaaagattt
     9301 ttaccaatga ttataggttt tttatttact ttcttttttg taaatatctt tgctattatg
     9361 atatttgcta atgaagtacc aagtagtgaa gataaagaat atggtgaaac aaaaaatagc
     9421 atagataaat atatagatgg tcaattagac aagctagata taaatgagat tcaagactat
     9481 ataaataaag aaatagttat taatgatgtt aatttaaaat catttgtaaa agatttaatt
     9541 agtggagaaa aaaatatctt agatttattc aataaagatg gattgaagat attgatgttt
     9601 gatgaattta aagcaagttt aaaggtagta gcagtaattt tggtactggc tctgttatca
     9661 tctattttaa agagtctgga aaactctttt tcatcaggtg cagtaagtca aatagcaaca
     9721 tatataatat ttatcacaat ggtatcactt acacttattg gttttaaaga tgtattacag
     9781 atttgttatg atgcaataga ccatactgta ggtcttatgc aagttataat gccaatatta
     9841 ataacatttt tacttttaat tgggttccca ataacgtcga ctactttgaa tcctatcttc
     9901 ataggaggag taacatttat aaatgtattt tttaaaaatt ttctatttgt atctattact
     9961 gttgcatttg gaattttaat aataaacaat ttatcaaaaa atataaggct aaagagattt
    10021 ttctcctttg taaaacaaat aaattatgtt tctattggag ctatgtttac tgtttactta
    10081 ggtcttgtat ccatacaagg attgtatgta accagttttg ataaattcag tgtaaagact
    10141 gctaaatttg ctataggtaa ttttattcct gttgtaggtg gatttgtatc tgactctgtg
    10201 gatattttat tatcttcatc tcagcttata aaaaatatat ttggaggaat agggcttatc
    10261 ttacttgttg gaatttgttt acttccagta ataaaaattt tatcagtaat agtagtgtac
    10321 aaattagcag caataattgt agagcctgtt ggagaggatg gaatatctaa ttttttaaat
    10381 gaagtggcaa atttaatgat aataatgcta gcatcagtaa ttgctataac agtaatgttc
    10441 tttgtaactg tagctatctt gacctcgata agtgtcgtaa gtcaagggta atttatatat
    10501 cagtgaaaaa taattacatt taatctaatg tttttagaga ggaggatgct atgttagagg
    10561 gcataaaggc atggatagtt agtgttttga taggagcatt tatagtaaat atagtagata
    10621 tgattttacc aagttctaag ataaaacctt atgtcaacct agttttaaac tttatgtttg
    10681 tatttattgt aataactcca gtagtaggat ttttttcaaa agatatgagt ttagaagata
    10741 gaatattaaa atctatggga aactacaaca agcagtatgt agatagtaca aatgctttgg
    10801 caaaagagac tggaaataat agtctatcca agggatatga ggatgggtta aaagaagtac
    10861 ttaaattaaa acttgatgaa tatggttatg atttagaaga tatagagctt aatggggcaa
    10921 atataaataa tataaaaata aaagaaaaga ataatagtgc aaaaagtaat aacagttcca
    10981 atgaagaaaa taaaaataat agtactaaaa gagaggaaga aaacataaac tctaatgaca
    11041 aagaaaattc taaacaagtc ttcaaaaaag gtacagagta tgggttgaac ctaaatgaag
    11101 aaaaattaaa aaacgactta ataaaggtgc tggatgtttc tatagaagat atacaaattg
    11161 ataaatagga gttgggagaa tgttgaaaaa cttaaatgac aaagataaga gaaaggtata
    11221 ttcactaata actatagcag gaatatgtgt ggtttcatta gtattattat cttgttttcc
    11281 tagtagcaaa agtgataaac aagttggaaa aacagagact aataaaaatg ctgaaaagca
    11341 agtcactaaa aaacaagaaa aagatgattt agagtcaaaa ttaacagcaa tactatctaa
    11401 aattgatggt gcaggagacg ttgatgtaat ggttacattt gaatcaagtg aagaaataca
    11461 acctgctttt aattcaaata atacaacaga aacaacagag gaaaaagatg cacaaggtgg
    11521 agaaagaaca gttacgactt ctagtgaaaa taaaacaatg ataacttcaa attcaagtga
    11581 ccctgtagtt ataaaaacta cagagccaaa gataaaggga gtgatagtgg tagcaagtgg
    11641 agcaagtgac ccaagtgtta aagagacact ttatagtgca gtacaaacat cattacaagt
    11701 agctggtcat caagtagaaa tatattctaa ataaatacat ataaccgggg ggataaatga
    11761 atgaagttta attataaggg aagaggattt gtaataataa ctttaactgc aatgttagtg
    11821 gtagtaggaa cagtgaatta tcaactaagt aagaaatctt tattagaaac atcaaaagag
    11881 tttaaggcat atgaagaagc acagttgcaa aaaaatactg atgatagtga tagtagtaag
    11941 acagaggatt ctagtaatgt agataaacaa ggtggtaagg aaagtgctga tattgatata
    12001 gtcgatagca aagctagtaa agtaaaagaa aaggcaactg agacaagtaa ggaaataaaa
    12061 gctcaattat catcagagaa aaatatgaaa aaggcatctt atattttaga tatgaaaatg
    12121 aatagagaaa aacaaagaaa tgagttagtt caagatttaa atgaaatgat aaataatcca
    12181 tctacaacag aagaatctag aaaagaagct tctaatatga aattaaatat agttaaaatt
    12241 caagaaaaag aacttcaaat agaaaacctt ttgagtacaa aaggatatga agaagcgtta
    12301 gtttatataa gcgataacaa agttaatgta gttgtaaatg aagctaaatt agaaaagaaa
    12361 gatgcagcaa aaatatttga tttagttgca gaacaagcca atgtgaagta tgaaaatatt
    12421 aaacttacaa ataataatag taataagtaa agtataaacc tcagaattaa ctgaggttat
    12481 tttcttgcaa agtagacttg tatttggtat tattatactt ataaaaatag ttccttaaaa
    12541 tcaggaggta agttttaata tggaagataa taaatttgga caagtaaaaa tatctaatga
    12601 tgtaatagct acaatagctg gtttagcagc attagaagtt gaaggcatag aaactacagc
    12661 aacattaaca gataagttgt taaaaaataa tggagtaaaa atacaaatcg aagaagagga
    12721 tgttaattta gatgtaatgg ttacgataaa gtatggaatg tcaataccag atacagcttt
    12781 taaagttcaa gaaaatgtaa aaaatactgt tgagacaatg acaggactga aggtttctca
    12841 agtaaatata cacattcaag gaatcagctt taagaaggat aaagttgata aagaagaagc
    12901 aaaagcaact aagaaaaatt aatattagcc ctctgaaaag tcagagggtt ttttgctggt
    12961 aattgatttt atatggataa ttaaacttaa aggagaattt tgattgatga aaaaagatag
    13021 ggcacaaaag agtacaacaa gagaatatat aatgaaactt atataccaaa taaatattaa
    13081 taaagaagat tttgaaactt tagaagataa agtagataat tttttaaaag ataattcaga
    13141 acatattata aatagatata aagaacttgc acttcaatac tcaaaaaata caaatttaaa
    13201 gttagaagat actgaaattg aagatgttat agacaagaaa tatataaata cagtatgcaa
    13261 agcattaaaa gaaaatcatg acaaaataga tgagttaata aataagcatg ctaaaaactg
    13321 gacagtagat agaatgccaa aggtagatgt ttctatactt agattgtctg tgtgtgaaat
    13381 actttattta gacactccta ataaagtatc aataaatgaa gcggtagaac ttgcaaaaat
    13441 atattgtgat gataagtctc ctaaatttat aaatggtata ttgggaagtg ttgttgatga
    13501 aattgggaaa taatataata attggaattg atactagctg ctatactact tctatagcag
    13561 ctatctcttt agataagaaa gttatattca atgaaaaaat aatgttagaa gtaagggata
    13621 attcaaaagg tttaaggcaa agtgaagctg tatttcaaca tatcaataat ttagggattc
    13681 ttagtgatag gataaaatct tttaaagata agtttaatgt agaaggagtt tgctcatcaa
    13741 aaaaaccgag acctgttgaa aactcatata tgcctgtatt caatgtaggt cataattttg
    13801 gaaaattatt gtctagtata tatggttgta ggttttatga gactactcat caagagaatc
    13861 atattgaagc aagtttatta aatagtaaat taaaaaataa taataaattt atttctgttc
    13921 atatgtctgg tggtactaca gagatacttt taacaagcaa acaagatagt catcacaatg
    13981 tatgtgatac taatttaggt aaaattgcca aaataagtat taaaaaagat gataaaagta
    14041 agttatacaa taattttgga tataatatag atataattgg aggaagtaaa gatattagct
    14101 ttggtcaact catagataga gtaggaatta agcttggata taaatttcct tcaggaaagt
    14161 atttggatga aaatgcttta aattgtaatc ttaaaataga aagtggtctg aaaacttctg
    14221 taagagatgg gtatatgaat ttatctggat tagaaaatca ggtaaataag attataaatg
    14281 ataatggaga taatactaat caaaaagaat acatatctaa attagtatta gattcagtag
    14341 ttagaaatat gtttaaatct ttagtatatt tatgtgaaac ttataatgta aatgaagtag
    14401 tatttgctgg tggagtttct gcaagtaagt atattcttag agaattaagc atgaagttaa
    14461 gaaaaaagca tatagaagct tattttacag aaccacaata ttcaactgat aatgctgtcg
    14521 ggtgtgctat aataggattg aataactttt taggagaaag agtatgaaac taagagcttt
    14581 agatataagt gaagcaaact catatattaa aagaatttta ataaacgacc caatactttc
    14641 taatcttaaa gttaaaggag aaatttctaa ttttaaggtt catagtagtg ggaatgtata
    14701 tttatcactt aaagatgaaa catcaaagtt aaactgtgta atttttaaaa gtaattttaa
    14761 tcgcaattta aaattagata atggtgtaaa aattatagct aatggatata tatcagtcta
    14821 tgaaagagat ggtgcgtatc aattatacat taatgaaatt gaaatagagg gaataggaaa
    14881 tttacacata gagtttaata gattaaagga aaaacttaat aaagaaggat tatttgaccc
    14941 taaatacaag atacctatac ctaaaatgcc aaactcaatt ggcgttataa catctccaac
    15001 aggtgctgtt ataagggata taataaatgt aataaaaaga agatatccga aagttaatat
    15061 aaagctttat ccagtaatgg ttcaaggaga taaatcagct gaagaaatct gtgaagcaat
    15121 tagatttttc aatcatatga agaatgttga tacacttata gttggtagag gtgggggttc
    15181 aatagaagaa ctttggtctt ttaatgatga aatggtagca agagaggttt ttaattctca
    15241 aattccgata atatcagcag ttgggcatga aacggatttt actatttgtg actttgtgtc
    15301 tgatatgaga gccccaacgc catcagcagc agctgagatt gctactccat cattggatga
    15361 tataaactat aaacttggaa acataaaaag taggatgagt aagtctctta caaatcaaat
    15421 agagctagac caatataggt tagaaacagt atttaataag ataaataact atttagattc
    15481 gtacacaata aaagataaag ttatacaatt agacaaaata tatgataaaa taatttttgg
    15541 aatagaaaat aatttaaaat tagaagatga aaaattagta aaaattggag cattactcca
    15601 taatttaagt ccattagcta ctatggatag aggttatagt ataactcaaa aaaatggaaa
    15661 agtaataaat agtataaaag gattaaaaat aaaggatagt atagatatag tattaaaaga
    15721 tggaaattta gaatgtatga tagataaaat tgaaaataaa gagggatagt atgaatttaa
    15781 cctacgaaga agcttataaa agattagaaa gtattttaag tgaattagaa tctaaaaatg
    15841 ctagcttgga tgaatcttta agcctatatg aagaaggtat aagcctttat aaacattgta
    15901 ataagctttt agatgatgct aaattgaaaa taagtaaatt taatcaatta ggaatagaag
    15961 aagactttaa aatagaggag gaataaaatt ggaatttaaa caatgtctaa aagaaaaagc
    16021 aagttttgta gaaaaagtac ttaaagaata tatgcctaaa gaagaagggt atcaaaaaac
    16081 tgttattgaa gctatgaact atagtttaag tgctggaggt aaaagattaa gaccaatact
    16141 tacattggag gcttgtaaaa tagttggagg aaatgaagat gaagctattc cttttgctat
    16201 tgcaattgaa atgatacata catattcact aatacatgat gatttgccag ctcttgacaa
    16261 tgatgattta agaagaggaa gaccaacaaa tcataaagta tatggagaag ctatggggat
    16321 tttagcagga gatgctttgt taaactatgc ttttgaagta atgcttgcag gctcaatcaa
    16381 taaagaaaat ccagaaaagt atctaaaagc tataaatgaa attgccaaag gtgctggtat
    16441 atatggaatg ataggtggtc aagtagtaga tgtagaaagc gaaaataaac aaattgaaaa
    16501 agaaaaatta gattatatac atatgaataa aactgctgca atgatggtag gctgtatgag
    16561 agcaggagct actataggtg gagcaaattc tgaacaaatg gaagaaataa ctaaatatgc
    16621 aaaaaatatc ggattatcat tccagatagt agatgatata ctagacatag taggagatga
    16681 agctaagtta ggtaaaaagg ttggaagtga tatagaaaat cataagtcaa catacccatc
    16741 tcttttaggt ttagataaat ctaaagaaat tgcacataat ttgattgatg aagcaaagaa
    16801 aagtatagaa aaattatctg atgatgtaga ttttttaaag ggattggctg aatatataat
    16861 agatagagaa tactaaaata tacagctaaa atcgaggagg gaaaaatgga ttttttttcg
    16921 gagattttta acaatggggc tttgggaata agtctgattg cttgtttctt agcacaattt
    16981 ataaaaatat ttactggtaa ggaaaaaaga attgagttat caagaatact tatttcagga
    17041 ggtatgccta gttcccatag ttcttttgtt acaagtttag ctacagtggt aggtattgaa
    17101 aaagggttta attctactga ttttgctata attaccgtac ttgccttaat tataatgtat
    17161 gatgcagcag gtgttagacg agctgttggt aaacaagcta ctatattaaa tcaaatggtg
    17221 gcagatatac agcatggaaa gcatatagag cagaagaaat taaaagaatt aataggtcat
    17281 acaccactag aagtttggtt tggagcttta cttggaatag taacagcttt aatattgatg
    17341 taaattgaaa atttacagaa taaaggtgag gataatatgt ataaatattt agataaagta
    17401 aattctccaa aggatataaa aaatatgagt atagaagaaa tggatttact tgcaaaggat
    17461 ataagaaagt tcttagtaaa atctgtttca aagacaggag gacacttagc ttctaatcta
    17521 ggagtggttg agttaacatt agcacttcat aaagtatttg atagccctaa agataagatt
    17581 gtatgggatg tagggcatca atcttatgtt cataaaatag ttactggaag aaaagattgt
    17641 tttgtatctt tgagacagtt taatggacta agtggttttc caaaggagaa tgaaagtcct
    17701 catgatattt ttgatacagg acatagcagt acatctattt ctatagctac aggtattgcc
    17761 tgcgctaggg atataaaaaa agaaaactat agtgttatat ctgtgatagg agatggttct
    17821 ataacaggag gtatggcact tgaagcttta aatcaattag gatatataga taccaatatg
    17881 atagtaatcc ttaatgataa tgagatgtcc atagataaaa atgttggagg aatgtctaaa
    17941 tatctgtcaa gtattataag aaactcaaca gttgagaaaa tgacagatga agttgataaa
    18001 attttaaatg ttacacaaac aggtgaaatt ttatctaaga ctgcacatag atttaaagat
    18061 aaactaatgt atagcttttc tcctcaggac tgttcatttt ttgattcatt aggtataaga
    18121 tattatggac ctatagatgg ccataatact aaagaactaa tagatatatt gagaaaagct
    18181 aaacacaaaa aagggcctgt tcttttacat gtaattacga aaaaaggaaa agggtacaga
    18241 tttgcagaag aacaacctga taaatatcat ggagtatcaa agtttgatat aaagacaggt
    18301 gttacatctg caaaagttaa gtctatgtca attagtgttg gagaaaaact tgttgatatg
    18361 gctaataata atgaagatat agtagcaata acagctgcaa tgccatctgg tacagggtta
    18421 aacctgtttg aaagtgcata tccaaaaaga tattatgatg ttggtatagc tgaacaacat
    18481 gccacaggat ttgcagcagg tcttgcaaaa aatggtatga aaccttactt tgctgtgtac
    18541 tcatcatttt tgcaaagggc atatgaccaa gttattcatg atgtatgcat aacaaaaaag
    18601 ccagttactt ttcttataga tagagctgga ttagttggaa atgatggaga aactcatcat
    18661 ggtatgtttg atttaagtta cttaaattct attccaaata ttgtagtaat ggctccaaaa
    18721 gacacaagag agatggagct tatgatggat ttgtcattaa aattagattg tccactagca
    18781 attagatacc caagaggaag tagttattac ctagataagg gagaatatgg agagattgta
    18841 ctgggtaaat atgaggtatt agatgacggt caagatacag tcatactttg tattggaagt
    18901 atggtaaaac atgctttaga ggctaaagaa atattatcaa gagaaggaat aaatccgaca
    18961 atagttaatg ctagattctt aaagccaatt gatgaaggta tgttaaaagc attattaaaa
    19021 aatcataaaa atgtagttac tatagaggat aatattgtaa ctggaggatt tggaagtaga
    19081 ataaacaagt ttattataga taatgaatat aacgtaaata tacttaatat agcaatacca
    19141 gaagaatttg taaaacatgg caatatagat gaattatatg attttgtagg attatcgcca
    19201 aaaagtattg ctgataaaat aaggaaatta gttattgagt aaaaatctaa tgtagatatt
    19261 gtaaattaaa atttatataa aatatagaat ttatcaatta gaataaatat caagtgtgtt
    19321 acttcaagct actttagtta tcattaaagt agcttttaac agaaaggtaa ttgtatgaaa
    19381 aaaagaatag atttattatt agtagaacaa gggtactttg aaagtagaga aagagccaaa
    19441 aaagcaataa tggctggatt ggtttttgtt gataatcaaa ggtgtgataa ggctggtaca
    19501 gaagtaaaag aagattgtag tatagaagta aaaggaaatc caataccata tgtcagtaga
    19561 ggtggtttaa agcttgaaaa agcaatgaaa aactttgatt tgactattga tggaaaggta
    19621 tgtatggata taggggcatc cacaggtggt tttacagatt gtatgctcaa aaatggagca
    19681 ataaaagtat tttctataga tgttggttat ggtcaacttg catggaaatt gagacaagat
    19741 gatagggttg tgtgtatgga aagaactaat ataagaaatg ttacaataga atatacaaag
    19801 cagtttgctg attttgcgtc aattgatgtg tcttttatat cgttaaagtt agtacttcca
    19861 aaggcaaagg agttagtacg tcatgatggg gaagttgtcg ctttaattaa acctcaattt
    19921 gaagcaggaa gagaaaaagt aggtaaaaaa ggagtagtta gagaaaaatc tacacatata
    19981 gaagttataa aaatgatttc tgatttctca gtcgaaaatg gatttgagat tttagggtta
    20041 gatttttctc ctattaaagg tcctgaaggt aatatagaat atctaattca tttgagaaat
    20101 ggaaatgaag gatatgaatt tgatggtgag acatacaata acaaaatagt agaagttgtt
    20161 gaagcttctc ataatttaga caaataggtt aaaagggggc ttagatttga tcctcgaatt
    20221 atatatgaaa aattgtgcct tagttgaaga actaaggttg aatattgata aaaacttaaa
    20281 tatacttaca ggtgaaactg gttcaggaaa atctataata atagatgcac ttggactttg
    20341 tttaggtgat aaatatgata ggtctttttt aagaaaagga acagataagg gacttgttga
    20401 ggctgtattt ttttcagaca atagatatct taaaaaaata ttagaagaaa atgatataag
    20461 tatggaagat gataatttat tggtaataac taggttgata tactcagatg gtaagagtac
    20521 tgctagagta aatggaagga ctgttaaagt gtctcttctt aaagaaattg catctacact
    20581 tatagatata catggtcaac atcaaaatca agctttgttc aataaagata ctcatttaaa
    20641 atttctagac ctttttggag aaaatgaatt ggaagagttt aaaattgctt ataaaaatgt
    20701 gtaccataag tatagtgaag taaaaaaagc tttaaattgc ttgacagaaa ataaagatga
    20761 gatgcaaatt caacgagaaa tagatttatt aagatttcaa ataaatgaaa ttgaagctgc
    20821 aaatctaaat aagaatgaat atgaagattt attaaaacaa agagaagttt atagaaatag
    20881 tgaaaagata tataataatc taaattcgag ttatagcaag ttacataatg gtgagtataa
    20941 tgttattgac ctaataggtc ttgcatctaa ggaacttaat gacatatcta aatatgacag
    21001 tgttttaagt gaatatagtg atactgtaga aagaattatg tatgagttac aagacatatc
    21061 tggtgagata agaaattaca aagataatat agactttgaa ccttatgagc tagagcagat
    21121 tgagcttaga atcgacgaaa ttaataattt aagaagaaaa tatggagaca gtatagatga
    21181 tatatttgag tattatggaa aaattaaaga tagattagat gaaatattaa atagagatga
    21241 aagagttgaa cagttaaaaa gcaaactgat gaatatagaa gaagatttaa aaataaaagc
    21301 cagtaaattg actaaagcaa gaatagaagt agcaactact ttagaaaaaa tacttcttga
    21361 tgaattaaaa agcttaaata tgaaaaatgt aatgttcaag gttaattttg aaaaaggttc
    21421 atttacttta gatggagtag atgatattga atttatgata tcttttaact taggagaaga
    21481 tattaagcct atatataaag ttgcatcagg tggtgagatg tccagattta tgttagcgtt
    21541 taaaaccata ttggctgata tagatgatat tgatacatta gtgtttgatg agatagatac
    21601 tggaataagt gggatagcag cacagatagt tggtgagaaa ttgagtgata tagcaaagaa
    21661 aaaacagata atatgtataa ctcatttacc tcaaattgct gcaaatgcag acactcatta
    21721 ttgtatagag aaagatactt caaataatag gacgtttacc aatgttagta agttaaatga
    21781 atctcaacga aaaaatgaaa ttgccagact tatagcaggt aacaatataa cagaaaaaac
    21841 tattgaacat gcaagtgaaa tcattgaatt ggctaaaaaa tgttagtgaa aaacaagcat
    21901 tttaataggt gtaaagaatt tttatagaaa tgctttacac ctatttttta tgatgaaatt
    21961 attttaaaat atctagtgcc tatattgaag aaaagtaggt ttgaaaatgg aatgaacttt
    22021 gtataaattt gtaactttga aatttctaaa tgcttaagta taatattatt tattaaggta
    22081 gtagtgaggg gaggcataaa atgaataaaa aagatactat gcacttaatt tttataattg
    22141 taatttttat tttattatgg tatattgtgg tagcagtaag tgagattaat aaaaatattg
    22201 aaagttgtag taacaaatta gattatttgt cacaagagct tatgagtact aagtcggata
    22261 ttagtaatac tataaatgaa gaaatgtcaa aaagttatat aacaaaaagt attgacttaa
    22321 aagtaaaaaa aatcgagaaa aaagaatgta ctattgatgt agatgttcaa ttaagtaaac
    22381 ttggaaaaaa tggaaaagta ttttttatgt ataaagaaga tagtgataaa tggaatgaaa
    22441 ctgagatgac aaaacatggg gaattatcat atgcatgtga aataaagata aatacaggaa
    22501 gtgaatatga ttataaagta gttacaagtg gagatatatc tgaaagttct gatgtacaaa
    22561 aactgactaa atctgattat atgcctgaac aacctatatt taattctggg ataagagata
    22621 atagagaata ctttattgaa atagtagaaa atagtaatct ttttaatcat gagagtgaaa
    22681 agtcaaaaaa taaagcttac gataatatca ggttagaaaa agtagatatt attgtaaatg
    22741 aaggaaaaaa agacactata tacaaagccc aattagctga aggcttagat gatggcaaaa
    22801 ctaataattc aaatagaaat caagtgaatt atgaagtaag ttttcctaaa agggatattg
    22861 atgacataat ttatattaaa gctaaactta catacaataa tggagtagaa tatacaaagg
    22921 atataactga agatataact atgggattac aagatttaga aaaataaata catatttaaa
    22981 taaatattca aatagatttg ttcacaaata gagtataaat aaaactgtag tagttttact
    23041 tatactctat taaaaaatta taattttaat tccatatatc tagccccttc aattggatta
    23101 taagtataaa gttctatatc ataaaaacca aacttttcat ataatccttg tgcacttttc
    23161 atgcttggaa gagtatctaa tctcatgtgt gtatatccaa tttttttagc ttctacaatg
    23221 atttcttcca atagtatttt tcctattttt aatcctctaa attgatttct tacatataat
    23281 ctttttaatt cacatatttt accttcaagt ttttttaaag caacacaacc tgccaaattt
    23341 tcatcaacaa atgccaatat caatgagcct gaaggttttt tatattttcc tggaagtgtt
    23401 tttaattcat tattaaagtc ttgaaaacat aagtctatat ttagagaatt tgaatattca
    23461 gtaaatagaa gttttacgtt ttcaagattt tctaaatctt tttcttcaac aaattgtaaa
    23521 gttatatttt gagccatttt atccgccatt atataaacat gtttatataa tgacttcacc
    23581 tccttgatat atgtatattt ttcttaatat caattagatt ttaataccat tactttttca
    23641 ccaccatagt caagctctcc attaaactta aatccaaaat ctttataaag cttaatagcc
    23701 attttattgt cttcgaatat actaagatat agttcatcac agttatattc atttttaaga
    23761 tgatttacaa gaaaatctaa tgatgctttt ccacatcctt taccttgata ttttgaatca
    23821 atcataaatc tatcaagcca aactctatca tcttctaaac gaccatacat agcaaatcct
    23881 attaaagaat tttcatcata tattccaaca ggtacccaag ctgttataaa tttagattca
    23941 gcaatagaaa gtgcattgct ttctatgtaa tctaactgac ttttatctac agacaaacta
    24001 actaaatcgt gccaattatt tatgtctgca attcttacat ttaatttcaa gatattaccg
    24061 tccttcaaac tttttgttac aatctaatac tatcatatta tgtattaaaa atcacttatt
    24121 aagattttaa ataaactttt tgaatgaata aattctcagg aaaaaggaat tataaataca
    24181 taataataca gcataggagt agatggatat atgaacaaaa taaataaaaa tttcaaaaat
    24241 atttggataa tattaggagc tgtatcgatt tttaccatgg ttataatttt taataaaaat
    24301 tgtaatgtta ataaagagac tcaaatagaa gcttttacaa ttaagaatca tgataaaaaa
    24361 tttgtatatc ctatgggaaa tataattgga gtaaaagcaa atacagatgg tgtattggtc
    24421 ttaggatatg aggaagagga tgtaggctat attggaggca tacaaatagg tgataatata
    24481 gtaaaaatag ataataaaag gataaaaaat agtcaagatg tatcagagat tttgaataag
    24541 attaaaaaaa gtaaagttga agtgactttt gagagaaaaa ataagtataa aacagaagta
    24601 atagagacta aaaaagaaaa tggcaagtat aaacttggat tatgggtaag agataaaata
    24661 tctggaattg gaactatgac tttttacgac cctagtatgg aaaagttcaa agcaatcggt
    24721 catgccataa aagattcgga tactaatgaa ctattaaaga taaagcaagg ctatatatat
    24781 aagccagaac aattaaaaat agtaaaagct agtaatgaaa aagtaggtaa aataaaagga
    24841 gattttaatg acagcaattt aatgggtaat ttctcaaata attcagagct aggtataagt
    24901 ggtaatatta cagaaaacca taataaagag tttaatgtag caaataaaga aaagcaatta
    24961 atagaagttg gaagaccgca agatgtaaaa ataggagatg cagtaattct ttttgaggat
    25021 aaaaacaaaa atataacaag ctatgatata aaaatagaaa gtatagtata tgataaagga
    25081 aattatagag atatggtaat aaaagtagta gatgacaagt tattggaata cacaggaggt
    25141 atcgtacagg ggatgagtgg agctccaata atacaaaata ataaaattat tggtgcaata
    25201 actcatgttt ttagagataa tccgaaaaaa ggatatggta tttttataga tgaaatgata
    25261 aaattgtagg tgaggcatta aaaaatttta ttattttatc aattatctag gaggaatata
    25321 attttggagt gtcgaatatg ctttagagta gataattagg aagcaattgt gtaaaaagtt
    25381 tagttttctg taataagaag atgtttttta atggggggat ttttagtgga aaaaatcaaa
    25441 atagttttag cagatgacaa taaggatttt tgtcaggtat taaaagagta tttgtctaat
    25501 gaagatgata tcgatatatt aggcatagct aaggatggaa ttgaagcatt agacttagta
    25561 aaaaagacac aaccggactt attaatacta gatgtaataa tgccacatct agatggatta
    25621 ggtgtaattg aaaaattgaa tactatggat atacctaaaa tgccaaaaat aatagtacta
    25681 tcagcagtag gtcaagataa gataactcaa agcgcaataa atctaggagc agattactac
    25741 atagtaaagc catttgattt tgttgtgttt ataaacagaa ttagagagct agtttctaat
    25801 agagttacac aagttgagcc aaaacctaga ccagttcaag agactcaaat gactagaagt
    25861 gattttgtta agaatgtagg aaatatagag acagaaatca caaatataat acatgaaata
    25921 ggagtaccag ctcatataaa aggatattta tatttaagag aagcaataaa aatggttatt
    25981 gataatgttg agcttttagg tgcagtaaca aaagagttat atccaagtat agctaaaaaa
    26041 ttcaatacaa caccaagtag agttgaaaga gcaataagac atgcaataga ggttgcatgg
    26101 agtagaggaa aagttgacac aataaatcaa ttatttggat atacggtaca caatactaaa
    26161 ggaaaaccaa ctaattcaga atttatagca atgattgctg ataaattaag actagaacat
    26221 agtatggtta aataaacaag acataaaaag taaggctttt taattaaggc attggctata
    26281 aatgcgtatt acaagcagcg aaacggtata accactaggg ttataccgtt tcgctatttt
    26341 aataaatata aaaattttct ttattatttg cttactatat caatataata attttattat
    26401 actatggata tagtatgtgt ctttacaagt tgtaaactga cagtggttta ttttttaata
    26461 taaatattga ctttgatgca ggtaaacttt gtatttttaa gcgtattatg gaatatgtta
    26521 aataaaaaaa tgatgaaata tagtattgta aatgccaaag atgcaaaaca aacttaaaac
    26581 atttatttta ttgttaagta atgctataat ataatgtgat tttaataatg atagtggagg
    26641 tttaaatatg agagtcgagg cccctataaa agtagatcga aaaaccaaaa aacttgctaa
    26701 aagagttgaa agtggggaaa tagcagttat aaatcatata gacatagatg aagttgctgc
    26761 aaactcttta gtagaagcta aaataaaact tgtcataaat gcggctcctt ctataagtgg
    26821 taggtatccc aataaaggtc cagggatatt aactgaaaat aatatactaa taattgataa
    26881 tgttggagaa gagttatttg aagaattaaa agaaggagaa actatagaag ttgtagatgg
    26941 aaagatatat agaaaaggaa aatttttagg tgcaggagaa gttttagaca agtatgaggt
    27001 ttcacaccaa ataaaggctg cctatgagaa tttagctgta gaattggata gatttataga
    27061 taatactata gattatgcta aaaaagaaaa aggatttata cttggagaag tagaaattcc
    27121 aaaagtgaaa actaattatg ctaataaaca tgtattgata gttgtaagag gtcaggacta
    27181 caaagaagat ttaagtacta tgctttctta tatagaagag gtaaagccag tattggttgg
    27241 tgttgatgga ggagcagacg ctttaattga gtttggatat actccagatg tgattgtagg
    27301 tgatatggat agtgtaagtg atgaagcttt aaaaaaggct agtgaaatag tagtacatgc
    27361 ttatacagat ggaagagcac caggtcttaa gagggttgag gaattaggct tagatgcagt
    27421 tgtatttcct gcgccaggaa ctagtgaaga tatagctatg cttatcgcct atgagtataa
    27481 ggctgagtta atagtagcag ttggtacaca ttcaaatatg attgattttc ttgaaaaagg
    27541 aagaaaaggt atggcaagta cttttttagt tagactaaaa ataggttcaa agcttataga
    27601 tgctaaaggc gttaatttgt tatacagaag taaattaaaa ataaagtaca tatgggcatt
    27661 aatagcaaca gcattatttc ctgtgttggt agttgcatca ttgtcaccag gagttcaaca
    27721 gttcatacaa ctaatgcaat taaagtttag agtattatta cagatgtaaa ttttaggagg
    27781 ataaatatgc atattaatat gaaatattat atagtcacta taggggctat atttatagct
    27841 ttgggtatag gcatgttagt aggatttaat ttaaataata atcaacaatt aagtgaacaa
    27901 caagcaaaca taataaatga tttggatgat aagtttaata tcttaaaaga aaaaaatgat
    27961 aaacttgaca gtgacttagc aagtgtaaat agagattatg aagaggcagt taattttata
    28021 aataaaaatg tagataagat tttagctggt tcgctgaatg gtaaaagtat aggaataata
    28081 tcggctaatg aaaatgacga ttatacaaaa aatatagaag acataataaa taaatctaat
    28141 ggaagtattg catttaatat agtattaaaa gaaaatataa caaatccaga aaagttaaag
    28201 gaaatgtcaa ctaaactagg tacagatgta aaaaatgcaa atgatgcagt taattatata
    28261 attgacaccc ttaaaaagga agatgcaagt gacattttaa catatcttca agaactagat
    28321 gtaataaagt ttaactctct tggaaataca tatctaaaat atgattctgt tgtaatagct
    28381 ggtggaaatg atgcaaaaga cagtacaaaa cagtttgaaa aaatagaaaa atttgtagtt
    28441 tcaaaactta aatcagagaa taaatattta gtagaagttc aaaatacagg agttaaaact
    28501 tcttatgtag aactatattc taaaaacaaa gttgcaacta tagacaatat tgatgaaggt
    28561 ataggaagta tatctctagc tattcttcta caacaaggta atatagttgg taattttggt
    28621 agattggata ctgcaacttc attgttgcca tctattaaat agaaataatt ttaaggagag
    28681 aaaaaatgga ttcttatata agtataataa taccagctta taatgaagaa agtagaataa
    28741 aatatacatt agaaagcata ttggacatta agcaaataaa tgaaataata gttgtggatg
    28801 atggttcaat tgacaatact gctaaggttg tatctgagct agaaaatgaa aagattaaat
    28861 tttttaagtt agataaaaat agaggtaaag gatatgcttt aaattatggt atcaaattag
    28921 caatgaaaaa tgcagatatt ataggttttc tggatggaga tttaggaagc tctgctaaag
    28981 aagttgaaaa attaatagta cctatattaa atggtgaggc ggatgttaca atagccaagt
    29041 ttcctcctgc aaagagaaaa ggaggtttgg gatttgtaaa aggtttagct aaaaattctg
    29101 tgtttgagat gactggtgtt gagttagatg caactttatc aggtcaaaga atatttaaaa
    29161 gagaagtttt agaaaagttt gatgaaatac cttttggata tggagtagaa gttggaatga
    29221 ccattgatat attaaagtat ggatttaaaa ttaaagaagt attagtaaat atgactcata
    29281 atgaaacagg aagaaattta aaaggtttta ttcatagagg gaaacagtat tatcacataa
    29341 aaaaggtttt aggacaaaaa aagaaagaat ggaggtaata tcttagtgaa agattatata
    29401 ctttatacca ttttattatt aactggatta tttggaacct atgctgtaat tccactattt
    29461 aaaaatttat tgataaatgg aaatgtactt agaccaaact ataaaaaaga tatgatacca
    29521 gtaagtatgg gaattgtatt tttgcctatg attgtaatca atggaataat acttgctttc
    29581 tttactacag aatttaagga tttagcgtat atatttatgt ttatgtttgg tatgatatcc
    29641 atgttttttg cagggatact agatgatgtc attggaaata gagatgtaag tggtttaaag
    29701 gggcatttta aaagtttatt taaaggaagt cttacaacag gaggttttaa agctttattt
    29761 ggtggatttg tagggcttat agtttctgtt gctatatcaa aagacataat tgatataata
    29821 gtaaatacac taataatagc tctatcaact aatttaatga atcttttgga tttaagacca
    29881 ggaagggcaa taaaagcata tttattcata atggtaatta tatttttaac attgacagga
    29941 tttgttcaag ttcttccatt acttatagta ccaaatgtac ttgcatactt taattatgat
    30001 ttaaaagcaa gggctatgat gggagatact ggttcaaacg tccttggaat atctattgga
    30061 atgctcattt cttttgggta tccatttaac attagattag gatggttaat atttgtagtt
    30121 attatgcata tattcactga gaaatactca ttaactaaga taatagaaaa aaataaacta
    30181 ttaaatttta tagataaatt gggaaggtag tattatgata agcaaaagag tggggattgt
    30241 tgaatcaata gtaagtcaaa ctgaaactct agatgatata agagtaaata taaatggaaa
    30301 aatacaaagg gcatataatt atcccaaaat atctggaact ataaatatag gtgatgaagt
    30361 ggttttaaat acaacagcag tagaactgag cttaggtaca ggtggatacc attttgtaat
    30421 aactaattta aataatatag aatctacttt aactgaaggt gggcatataa tgaaacttag
    30481 atatacacct cttcaaataa aagtggattc tgttgaggaa caagaaagta tttatcatga
    30541 taaatttgcg aattttaaag gattagaagg tctttctgtt gtagtaggga ctcttcatag
    30601 tatgttaact ccatttgcag cttcatttaa aagaaataat ccaaataaaa aattagtata
    30661 tataatgact gatggagcat ctcttccaat ttatttaagt aaaaatgtgg acactttaaa
    30721 agaaaaaaaa ttaatagata gtacgataac tataggaaat gcgtttggtg gagattatga
    30781 atgtataaat atatatactg cattaattac agcaaaagaa atactaaaag cggatgcagt
    30841 atttgtcagt atgggacctg gaattgctgg gacaggaaca aaatatggat ttacagggat
    30901 tgaacaaggc tctattcttg atgccgtaaa aaaacttgaa ggaagagcaa ttgcaattcc
    30961 aagaattagt tttgctgaca aacgagaaag acatcaaggt atttcacatc atagtatgac
    31021 tgtatttaaa gaaattgtaa atgtaaatgt agacatacca ataactatat atgatgatga
    31081 aaaattgaac tttataaaag agcaaatcaa tttaaatggt ttagacaaaa agcataatat
    31141 aatttatata gataataata aaacgaaaga ggatttagac tattttaatc taaaagtaaa
    31201 gagtatggga agaaattatg aacaagatga ggcatttttc aaagctgcaa gcacagcagc
    31261 atattactta atggaggttt aagatggtat tagaagaaaa aactattagt agtgataggg
    31321 tttatacagg taaagtaatt actttaaagg ttgatactgt tgaaattcca gggcaaggat
    31381 atcaaaaaag ggagttagta gaagttggtg gagcagtagg aatagttgca atcacagatg
    31441 ataataaagt tgtactagtt aagcaattta gaaaacctat agaaaagcca atctttgaaa
    31501 ttccagctgg taaactagaa aaaaatgaaa gtccaaaaga atgtgctgaa agagaattaa
    31561 aagaagaaac tgggtatagt gcaaaaaata ttaagctaat acataaattt ttcacttcag
    31621 caggtttttc taatgagata atgtttgtat atttagcaac aggtcttact ccaggagaga
    31681 ataatttaga tgcagatgag tttttagatg tctatgaaat tgagcttgaa gaagcatata
    31741 acatggtatt aaaaaatgac gttgaagatg caaaaacatc tattggatta ttattagtaa
    31801 aagatatgtt taaaaattaa tatttataaa atgaataacc ctttgtccta aagcatatta
    31861 ttttataaag gacaaggggg aattaaattg agaagaacat atagaaaaaa tgattttaga
    31921 gaattaaata gacaaataat tattatagga ttgttgttta tgatgtctat tgttgttggg
    31981 tcttacataa ataaaatatt gccaggttct agtaacaata tattaaacaa tataaatcct
    32041 gctgtagagt attataatct aaatataagc ataaaagata cagtgataca aaatttaaaa
    32101 tcagatgcaa tctttatggg ctcaatagct ctattaagtc tatttgtagt aacaattcca
    32161 gcagtgttgg tagcttttgt attaaaaggg atgtcaattg gttatacaat aaatagttgt
    32221 atattggcat taaaatttaa aagtataaaa atgattttaa taatattatt taaaaatttg
    32281 attattattc catgtgcaat catattagca ttaattagtt taagttattt taaagaaatg
    32341 gtttatgaat ttaaaaagaa aaatagaaaa aatatgcaat
//
LOCUS       DAELSY010000027        30967 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.027, whole genome shotgun sequence.
ACCESSION   DAELSY010000027 DAELSY010000000
VERSION     DAELSY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.027"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..739)
                     /locus_tag="KPG09_001167"
     CDS             complement(<1..739)
                     /locus_tag="KPG09_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG0965790.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTN"
     gene            905..3472
                     /locus_tag="KPG09_001168"
     CDS             905..3472
                     /locus_tag="KPG09_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0965791.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNNSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAVDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            3702..4106
                     /locus_tag="KPG09_001169"
     CDS             3702..4106
                     /locus_tag="KPG09_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0965792.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            4130..4627
                     /locus_tag="KPG09_001170"
     CDS             4130..4627
                     /locus_tag="KPG09_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0965793.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            4630..5031
                     /locus_tag="KPG09_001171"
     CDS             4630..5031
                     /locus_tag="KPG09_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBG0965794.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            5049..5537
                     /locus_tag="KPG09_001172"
     CDS             5049..5537
                     /locus_tag="KPG09_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0965795.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            5564..6319
                     /locus_tag="KPG09_001173"
     CDS             5564..6319
                     /locus_tag="KPG09_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0965796.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            6319..7182
                     /locus_tag="KPG09_001174"
     CDS             6319..7182
                     /locus_tag="KPG09_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0965797.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKKKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            7229..7708
                     /locus_tag="KPG09_001175"
     CDS             7229..7708
                     /locus_tag="KPG09_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBG0965798.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            7773..9080
                     /locus_tag="KPG09_001176"
     CDS             7773..9080
                     /locus_tag="KPG09_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0965799.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETDMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            9391..9531
                     /locus_tag="KPG09_001177"
     CDS             9391..9531
                     /locus_tag="KPG09_001177"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBG0965800.1"
                     /translation="MNGEGCILSNKITKECYKTGYMIREKSIDTYPDSGRGFMTWDEN
                     GE"
     gene            10043..11137
                     /locus_tag="KPG09_001178"
     CDS             10043..11137
                     /locus_tag="KPG09_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0965801.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQTQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSLLSENKEYKFLIEKLSLLEE"
     gene            11142..11342
                     /locus_tag="KPG09_001179"
     CDS             11142..11342
                     /locus_tag="KPG09_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBG0965802.1"
                     /translation="MENLMEYLPLLIPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            11342..12259
                     /locus_tag="KPG09_001180"
     CDS             11342..12259
                     /locus_tag="KPG09_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0965803.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLEPDSGTIDVCGEKVTYGRTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIKIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNNEIQKFKSSGGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            12256..13014
                     /locus_tag="KPG09_001181"
     CDS             12256..13014
                     /locus_tag="KPG09_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0965804.1"
                     /translation="MREYIAFTKKEFKENIRNYKLLSLTILFLVFGIMSPLSAKFMPD
                     LIAHFAPTLKVTTAPTALDSWTQFSGNISGLGTSLTLIVFCNILSNEYSKGTLVIMLT
                     KGLSRSSVVLSKFSIAVIIMTIGFWLSFLCTYGYTMYFWPTANLNHIIFSAFNLWLIS
                     IMYISILILGCVLFRPAFASVLLVLAATAILSLISIPKQIAPYTPNFILSKNIDLVSG
                     KVTPPEFIIPIIVTIVISIVCLLLAVVLFNKKAV"
     gene            13397..14470
                     /locus_tag="KPG09_001182"
     CDS             13397..14470
                     /locus_tag="KPG09_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG0965805.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            14540..14803
                     /locus_tag="KPG09_001183"
     CDS             14540..14803
                     /locus_tag="KPG09_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965806.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            15009..15986
                     /gene="bioB"
                     /locus_tag="KPG09_001184"
     CDS             15009..15986
                     /gene="bioB"
                     /locus_tag="KPG09_001184"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBG0965807.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMVFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            16214..17227
                     /locus_tag="KPG09_001185"
     CDS             16214..17227
                     /locus_tag="KPG09_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0965808.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNSAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDVSVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            17269..18159
                     /gene="rbsK"
                     /locus_tag="KPG09_001186"
     CDS             17269..18159
                     /gene="rbsK"
                     /locus_tag="KPG09_001186"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBG0965809.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIKVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKKLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            18207..19169
                     /locus_tag="KPG09_001187"
     CDS             18207..19169
                     /locus_tag="KPG09_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG0965810.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            19223..20734
                     /locus_tag="KPG09_001188"
     CDS             19223..20734
                     /locus_tag="KPG09_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG0965811.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            20718..21716
                     /locus_tag="KPG09_001189"
     CDS             20718..21716
                     /locus_tag="KPG09_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBG0965812.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            21900..23162
                     /locus_tag="KPG09_001190"
     CDS             21900..23162
                     /locus_tag="KPG09_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0965813.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIGGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFQMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            23182..23925
                     /locus_tag="KPG09_001191"
     CDS             23182..23925
                     /locus_tag="KPG09_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBG0965814.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            24098..25483
                     /locus_tag="KPG09_001192"
     CDS             24098..25483
                     /locus_tag="KPG09_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0965815.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFEYTPII"
     gene            complement(25820..26464)
                     /locus_tag="KPG09_001193"
     CDS             complement(25820..26464)
                     /locus_tag="KPG09_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0965816.1"
                     /translation="MNNLDKNNVNEVLDMLLRTRRSIRSFTKEVPSKGLIEEIIETGR
                     LAPYAILSVAKQTDFRHFFVISNNSLQMNKIKTILVDVIKTQISVNEKKAKNNITLQS
                     FITSLKMQVEKGVLGIGTAPYLIIVAERRGIPPIEQKSLSHVMQNMWLKSTALGLGFE
                     LVSVIGRLSDNKDFCDILGVPTGEYGFDACAIGFPTTSVTREGRAIPHPSIKWI"
     gene            26728..27342
                     /locus_tag="KPG09_001194"
     CDS             26728..27342
                     /locus_tag="KPG09_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBG0965817.1"
                     /translation="MKKVSIDRRTIRTKKMIRSALAELIDEKGFNNISVTDLTQRADI
                     NRGTFYLHYVDKYDLLERIENEIIQEIEEETKHLDSSNMMEIDTSSEPLPFMVKLFEY
                     FRKNSVIIKSILGPKGDPTFERKIKKFIETNLFEKQKLINFNIDEAIISEEYFIQYIL
                     SADLGVIQYWLEKNMKESPEEMALILARMSLLGPLRAVGIRKDS"
     gene            27563..27703
                     /locus_tag="KPG09_001195"
     CDS             27563..27703
                     /locus_tag="KPG09_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965818.1"
                     /translation="MKNENISYLALGLCFGVVLGSLFKNLAIGLSFGLVIGVVLDSKK
                     NK"
     gene            complement(27933..28380)
                     /locus_tag="KPG09_001196"
                     /pseudo
     CDS             complement(27933..28380)
                     /locus_tag="KPG09_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599264.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            29150..29989
                     /locus_tag="KPG09_001197"
     CDS             29150..29989
                     /locus_tag="KPG09_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965819.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            29979..30422
                     /locus_tag="KPG09_001198"
     CDS             29979..30422
                     /locus_tag="KPG09_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965820.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            30433..30924
                     /locus_tag="KPG09_001199"
     CDS             30433..30924
                     /locus_tag="KPG09_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965821.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
BASE COUNT        12142 a         3200 c         5682 g         9943 t
ORIGIN      
        1 tatttgtata atcaacatct agtaatttac ctttttggtg aaaatgatta ataagttttt
       61 taggataatt cattattaat tcatctggaa aattaatttc ctttaagata ttctcagaat
      121 actcatagtt acctatgtca aaacaaatgt gagcatctga agttagtatg attttagcac
      181 catatttttt gcaaagtgta gcaatatact tacagttatc cgagctacca tttcttgaca
      241 cgcctttaat agaacaatta tttatttcta ttaagatatt cttttctata gctaatttaa
      301 tgattaattc atagtcgatt gggtagtttg ggtttccaag gtgtcctaat atttcaatat
      361 tatcatgctt atttattgca tttattaaag ctttagtatt attctctaat gtatttggag
      421 aaaaaacagc ttcgtgaaat gacaagataa gataatctaa ccgaggaatt acaaaaggct
      481 ctaggtctat gtttccttta atatctagta tatttgcttc acagcctctt aaaattataa
      541 tgttatttat gattcttggg atattttgaa tgttgtgaaa gtaccatctg tggggagaac
      601 caggcatctt aggaccatgt tcagatgtac ctagtatctt aattccattt tctttacaat
      661 aattaatatt ttctagtaaa gtgctatagc catgaccact gactatagag tgggtgtgta
      721 aatctgataa aaaattcata taaattcctc ctctataaaa gatgagggta acgtaattta
      781 tattataaaa cgaataggaa agaaaatcaa tagttacata attagacaaa aatattaaaa
      841 tattgtagaa ttaagcttaa taataaatgt ttaacagtat tactgcttat aggggaggtt
      901 aactatgaca caaaagaagc tatcaaggaa agaaaaagtt ttaatttgct tgaaaaggtt
      961 aacagaagaa cacttgaact ttaataatgg cattagattt ggatttgatg cagaatatat
     1021 agctactgaa gtaggtataa atagaagtaa tgctagtaag gaattaaatc aattaataaa
     1081 agaaagtatt attataaaga taaagggtaa gcctgttcag tacttatata aagaaagtat
     1141 tgagagtatt ttgggaaaga aactaaataa agggatattt gataatatta actctataaa
     1201 cgaatatagt gaaaatgatt ctatcaaaga tgagtatatt ttagagagta taaaaaaaga
     1261 gaacaatatt tttaatttag taggatgtga tggaagttta aggtcccaaa tagaacaggc
     1321 taaagcagca gtaatatatc cacctaaggg gttgagtaca ttaataatag gacctacagg
     1381 agttggtaaa tctatatttg cagaatatat gtataagtat agtttaacta taaaagaaga
     1441 tagtgataat gcaccattta tcatatttaa ctgtgctgat tattctgata atcagcaatt
     1501 attattagcg cagctttttg gatatgtaaa aggagcattt actggagctg ataaagataa
     1561 gtatggtatt gtacatgaag ctaataatgg agtattgttt ttagatgaag ttcatagatt
     1621 atctgcagag ggacaagaaa tgctatttct gcttatggat aaagggatat atagaaggtt
     1681 aggagaggct aataaggtac acgagtgtag agttatgata attgcagcca ctacagaaga
     1741 cccagaaaca gcaatgttgg aaacgttttt aagaagaata cctgtaacta taaagattcc
     1801 gtctttagag gaaagaggct ttcaagagag aatgaagctt atatgttatt tttttaaaga
     1861 agagagtgtt aggattggtg ttaagttaaa ggtttcaaaa gaagttatta aggcatttat
     1921 tttatataag tgtaagggaa acataggcca attaaaaagc gatatacaat taatttgtgc
     1981 aagagctttt ttagattata tgacctataa aagggaatgt gtatatgtaa ggctctctct
     2041 tctaccaaat aatattagag aatcattata tggaaataat agaaaagagg agtttgtaca
     2101 aaatttcagt tttattggaa aagatgatat tattttttta ccagaagaaa aggattttga
     2161 aagtgaaaat ttattaatag aaaataaaaa atatgattta gatttttatg gaatgataaa
     2221 agaaacttgg aataaacttc aacaagaagg tattaaagag tcacaaatta gaagtgttat
     2281 agatggagac atacagaagt attcctataa tttaatgaat acttttatat ataataatag
     2341 taatcaaaca gcttataaca acattgttaa tgattcaatt gcaactactg taaaatatat
     2401 tttacaaaag catgataaat ggagcaaaag agaaggatta gataaattaa taaaagctat
     2461 agccttacat attcagaatt taattgaaag aataaagatt ggaaatatag taaagcatcc
     2521 taataaagat gaaattatga aggaaagagc atatgaatat aacatagcaa aagaaatatt
     2581 atctaacatg tcattaatgt atggagtaga gtttccagaa gatgaagcaa tttttttagc
     2641 aacatttctt tatttatcgt atgtgggatt aaatgaggag agtatagcaa tattagtcat
     2701 aatgcatggt gaatctacag catctagcat ggtgaatgta gccaatactt tactggattg
     2761 taaccatgca gtggcaatta atatgggatt agaagataga gttcaggata ttttagtaca
     2821 agctgtagaa atagccaata aaattgataa aggaaaggga attttaattt taactgatat
     2881 ggggtctatt ttaacatttg ctaaagttat tcgtgatgct acaggaaaag aagtaaaagc
     2941 agtagatatg gtaagtacac ctattgtaat agaagcaact agaaaggctc taactccaga
     3001 aatgacatta gagaaattat attttaatat tatagaatct ataaagaaac attatggaga
     3061 tatggaagtt acatatgctg aaaaagataa tggtggtaga tattttgata gactactaat
     3121 tgataatatt agtaaaactt tgactttttt aaatggggaa aaggcttatt ttatattaaa
     3181 agaagtaatt aatagaatta gtgaacacta ttctttagta attcaagatg aacttttagt
     3241 aaagtttata tttcattgtt catgtatgat tgaaagagta ataataaaag agtctttagt
     3301 ttataaatca tatgaagaaa gaattagtag aaataaagaa ttatatttag ttataaaaga
     3361 aagttttaag ctagtagaag agacattcga tataattatt tcagatatgg aatatgcaaa
     3421 tatattagat atatttgaat cacaatatga tacagataaa ttcaaaagtt gatacaaaat
     3481 ttaaaaatag ccaaaaggct atcttttttt tacttttttt gatacagatt tgatacaggt
     3541 tgaaaaaatc ttttgataca tatcgaaata ataagttttt aaaaaaaatt gagtaaaaag
     3601 ttttgattaa atcctgtaaa atagccattt attattgata cacataagtt ggcacggcaa
     3661 ttgcttatat aaaaagtgac aagaaaagtg aggtgaaatt ggtgatagga ataatagtta
     3721 tgactcatgg gagttttagt gaagaaatta taaaaagctg tgaacttatt gcaggaccag
     3781 cagaaagaac agcagcaatt aaactaaata ggaatgataa tattgaagac ttaaataaaa
     3841 attttgtaga aaagttaaat gaattagatg aaggtgacgg agttttagta ttagctgacc
     3901 tattaggagg ttcgccaagt aatgttgcaa gtttaaacct taaaaaaggt ggcaagtttc
     3961 atgcacttac tggagtaaat cttccaatgc tacttgaagc tttaattaat agagagggaa
     4021 aaagtttaga agagttagca gaagcatgta ttgaagcagg acaaactggt ataaacaata
     4081 ttaataaagt tcttagctca atgtaataga aaaaggagtt tgattaaaaa tgtctttagt
     4141 aaaatattgc agatgtgatg atagattaat acatggtcaa gtgatatata aatgggttaa
     4201 gcatctaggt gtaaaaaaga tagtagtagt agatgatgaa acaaccaatg atgttattgc
     4261 taaaggtttg ataaaaatgg cagcaccaaa gaatattgat ttatcaatat taactgtaag
     4321 tgaatcaagg agatattttt ataataatca agctgatgat aacgtttttg tattaattaa
     4381 aaatttagat actgcaaata gaatgataga agaagggatt aatatcaaaa aacttattgt
     4441 tggaagaata cctacaggaa taggaaaaaa gaaaatatct caaaatgtat acattaataa
     4501 aaaagagttt cttttaatag atgaatttat taaaaagaat attaatgtat caattcaaat
     4561 ggtacctgat gaagaagaag tagaattaaa ccaaaatatt aatcgctata aaggagagtt
     4621 tatttagcaa tgaataaata taaggtaatt atactgtgcc atggaacttg gggaaaagtc
     4681 ttagtagaag aaacaaataa aaactttgga ttgtcaaatc aatatgaagt tttatcacta
     4741 agttctgaaa aagatgtttc tgtatttatg aaagaggttg aagaagtagt agataaagaa
     4801 gatgtgaaaa ttataatatc agatttatat ggaggatcta catcaagtgt tgcaatagct
     4861 gttgctataa gaagaggaat agatgcaata aatggactta gcttacagac aattttaatc
     4921 gtagatgaag aattaagcaa agaagataaa ttacatacat taccagaaag aatcgtcaaa
     4981 aaaaataata acctttgtgt ggatttaatt aaagaattta aaaaaatata aattatgagg
     5041 tgaatataat ggcaaaaata acattagtaa gagtagacca cagattaatt catgggcaag
     5101 ttataacaaa atgggtaaaa atcgctcaag ctcaaaaaat aataatagtt gatgacttct
     5161 taggtcaaga tgaatttatg gctgacatat ataaaatggc agcaccatca ggagtagaag
     5221 tagttatttt aacagcagaa gatgcgggac aagcattcca aaacaacact ttaggagata
     5281 aaaatatatt tattctattt aaaaatgttg atatggcaaa taaggcttat aaagctggac
     5341 ttaaatatga aaagattcaa ttaggaggta ttccaaatga agcaggaaag aaaatggtgt
     5401 ttacagctgt ttcattagga aatgaggatg tagaacaact taatgaactt aatgaaaatg
     5461 gggttgaaat agtcctacaa gttataccag aggaatcatc tatgacatat gaaaatgcgt
     5521 taaagaaatt taaataaaaa atataaatta ggaggaatta taaatggaat ctgttttgat
     5581 gttggcaatt gtaacaggat tatggtattg gtttgcagca ggtcttgcag ggtataccct
     5641 tttctcaaca ctaaagtctc ctttatttat aggttttagc cttggattac tttggggtga
     5701 tgtaacaact ggtatgattg ttggtgcaag tatagaaatg gtttatctag gaatggtcgc
     5761 agcaggggga aatattccat cagataaatg tctagctgct cttattgcaa taccagtagc
     5821 acttcaaact ggtgttaatg cagaagttgc agtatctatt gcggtacctt taggtgttat
     5881 aggagtatta gttaataact taagaagaac tggtaatgct gtattggttc ataaggcaga
     5941 taagtatgct gaagaaggta atacaaaagg catctggaga tgtgcaacac tttattcttt
     6001 aatatttggg tttgttttaa gatttccaat agtttttgta tgtaactttt ttggagcaga
     6061 tttagttcaa tcattattag atgttatacc acaatggtta atgaatggat taacagtaat
     6121 gggcggtata ttacctgcat taggttttgc aacaacaata tttactatag gtaaaaataa
     6181 attcttacca atgtttatta taggattttt tatggttcaa tattttgaaa tatcaattac
     6241 tgcagctgca atatttggtg tttgtatagc tcttttaatt acctttatga aagaagacaa
     6301 aagggtaggt gaagtataat gcaagaaata aaaaataaag aagttaaaaa tggacaaggt
     6361 ccaactgaag ttaataagac aagagtactc acaaagaaag atgtaacaaa aacataccta
     6421 agatggtggt ggacagcaga gttatctaat tcttttgaaa gaatgcaagc attagcagtt
     6481 tgtgcaagtt tcactccggc tttagaaaag ttatataaga aaaaagaaga tttagtagat
     6541 gcattaaaga gacacttaca attttttaat actcaagcta tatggggagg attaattcat
     6601 ggtacagttt tagctatgga agaagagaaa gctactgaag gaaaaatccc aggagaagtt
     6661 atttcaggtg ttaagaatgg tcttatggga cctttggctg gaattggaga tactttagat
     6721 tttggtacat ttcaaacaat attcttagct ttaggtgctt catttggagc agaaggaagt
     6781 gttataggtg catttttccc aataatgttt agtatattac tattttgcga aggttattac
     6841 ttatttcatt taggatattc tttaggtaga gattcaatta agaaaattct atcaggtggt
     6901 attgtcaata agataattga tggtgcttca attctaggaa tgtttatgat gggagcatta
     6961 tctgcaacta cagttaagct ttcaactccg ctatcatttg atattggtgg taaagcaatt
     7021 gttgttcaag atacattaaa tatgattgct ccaggtttat taccacttgg tgttgtattc
     7081 ttcgtatatt ggggaatgaa atacaagaag tggacaataa ctaagttatt agtaatttta
     7141 gttgtattag cattggtagg atcattcatt ggaatattct aattcacagt gattaaaata
     7201 tagaatttaa ttttaggagg agaaagaaat gtcagaaaaa atgtatacag aaaaggaata
     7261 tttagaagga attagaggag caacaggaga aagagcagta tggttctatc ttcttatgaa
     7321 agaagctgaa aaattaggag ctaatccaga tgatatatgt aaagaagcaa tatatggatt
     7381 tggtaaaatg agaggtcaaa aatataacgt agcagatact ccaggaaaaa tggcagaaat
     7441 gttatataac agtaaaggac aaaaagtgtt tgaaatggaa ttagtagaaa atacagatga
     7501 gaatggcgtt ttaaaattcc atcactgtcc attagatgct gcttggaaag aatatggatt
     7561 aactaaagaa gaaagaaaag aaatttgtag attagcttgt tatggggatt atggaagagt
     7621 tgactgtgca caaggtgtta aattagattt tgctcaaaaa tgtgctcatg atgatgaagt
     7681 ttgtgaatta gtatttacaa aaaaataatt tttaatgcta aaagcctgtt ctttaaggac
     7741 aggctttaaa aagatagaaa gtaggttgat ttatggatat aagcagaagt gtagataata
     7801 gtttaaatag aaccatagaa tttctaaaag aattgataaa aattgattca caacaaggtg
     7861 aacctatttc acaatgtcct tttggtatag gacctaaaaa atctttagat aaaactttag
     7921 attactgtgc tagtttaggt tttagtgtta aaaatattga taattatatt ggatatgcag
     7981 aaattggaga gggagaggaa ttaataggga ttccaatgca tttagatata gttccaccag
     8041 gagaggggtg gagtgtagac ccattttcgg gagcagtaat tgacaatatt atttatggaa
     8101 gaggtgtcat agataataag ggtgctgtat ctatgcttat acatgtattg aaaaacatag
     8161 aggatatgta tcctacaatt aataaaagaa taagattaat ttttggtaca aatgaggaga
     8221 cagatatgaa atgtatcaaa tattacttag ataaaggtga agaaattcca agtatgggat
     8281 ttactccaga tgctatgtat ccagtggtta atggagaaaa aggaagggtt catataagga
     8341 tagaaaaaga aattaaaatt gacaaaagta aaccttacat aatagtaagg ggaggaacta
     8401 aagaaaatgt tgttccatct cattgtactg caaaaattat taatggaatt atttcagagt
     8461 taactactaa aggagtagct gtacatgcaa gtaatcctga aaagggagaa aatgctatat
     8521 ctaaaatggt aataaagata gttgaggata atatggattt tcagcataga gaagatatag
     8581 aattagtttc aaaatacctt tgtagtgatt attatggaga tgcattgggg attaatcaat
     8641 atgatgaggt attcaaaaac actaccttaa atttaggaat attaaaagta aatgaggaaa
     8701 aaatcgtatg tgagttagat ataaggtatg gtaaaaatat agttttaaat aacataatag
     8761 atagattcaa aaaggttttt tgtaatggtt ggaaaataga ggttattgct cataaagact
     8821 tacattatgt ggatgagagt aatttagtac ttaaaaaatt attagaagcc tatgaagaag
     8881 taacagatga aaatggatat acaattgcta tgggtggtgg cacatatgca agttggttta
     8941 aagatatggt ggcgtttgga cctaagttct tagcgtataa aactggtggt catggagtag
     9001 atgaaagagt accaataaat catatacgaa agaatatgga gatatatact ttggctttaa
     9061 taaaactatt agaattgtaa aagaagaaga gactttatgt gcaaatttac agatatctta
     9121 aaaagagaga taaaacgagt tcaactttat ttaaatgttc aacaccatca aatggggctg
     9181 tctcaaaata gagataagcc tcttgcttat tattgaaatg atgaaaatat attttacttc
     9241 atacaaaaaa gagtgcctta tgaagtaaaa agttaatttt gagacaccct catttgatga
     9301 tgttgaagag aaaataagta tgaaattatt aaaaaaagat gattttataa aagttaaggt
     9361 tgaaaataaa agttaattga atggaattag ataaatggag aaggctgtat actatctaat
     9421 aaaataacaa aagagtgtta taaaactggt tatatgatta gagaaaagtc tattgatact
     9481 tatccagata gtggacgggg atttatgact tgggatgaaa atggagaata ataaaaatta
     9541 tagtttatac aatttaataa gatatatgta caagcagtta atctaaatga ttgactgctt
     9601 ttcttttata gatttttata taatagacaa ttactaatac atgatagttg cctgttataa
     9661 ttagaggaat agatacgaat aataacttta taaaagaatt aaatggaaaa ttgaagagtt
     9721 atattcagga ttttaaaaag aatatgaaat gagaaatgaa aataaggtgt ttatattaaa
     9781 agaggtttta cacaaaatac ttaaaaacgt gataaataaa aactgtattt agataaatta
     9841 actactagtt ttatatataa tatggatttt gtttacaaag tttacatatt tcacaaaata
     9901 tttttaaaat atatttaaaa atatatttta tatacttgtt cgtttgtttc tctgtggttg
     9961 attttttcgt caaaagtcaa cttcaaatcg cttgttagct ttacatgttt aaggatataa
    10021 ttatacataa ggagggattg acatgaatga attaaacatc gcaaaaacgc tgattttaaa
    10081 gcgtaaagaa aaaggaatta cacaagatga acttgcaaat tatataggcg tttctaaagc
    10141 ttctgtatct aaatgggaaa ctggacaaag ctatcctgac ataacctttt taccgcaact
    10201 cgcgacatat ttcaacatta ccgttgatga gttaatatgt tatgaacctc aaatgatgaa
    10261 ggaagatatt aataaactgt ataataaact gtgcaaagac tttacggtta aaccgtttga
    10321 tgaagtcatg gatgaaataa gagaaatcat aaaaaaatat tattcatgtt ttcctttgat
    10381 ttttcggatg ggtcttttaa tagttaatca ctatgatatt gtagatgaaa aaaagcgtga
    10441 actattaatt agtgaagcat tagaaatatt tattcgtata caggaaactt gtaatgacat
    10501 agatatttgc cgtcaagcaa aaagtatgga ggcaacttgt tatatattgt taaatcaacc
    10561 catacaagtg attgatttat tgcaaaacag caattttccc atgataaatg aatctatact
    10621 tcttgcacag gggcaaatga tgaatggtca aatggatgaa gcaagagaaa catttcaact
    10681 tggtgcatat cagaacctta tatcattagt ccaaaatcta gtgggtattt tgcaaaatgc
    10741 agataaatta caaatgaagg aaatagaaag acgtattcta gcaatttcag atatatttga
    10801 gcttgataca ttatctcctg ctattatgct ttcttcatat ttaacacaga cacaaattaa
    10861 tttaatacat ggggataatg aaggagctat taaatcatta cgaaaatatg tagatttagc
    10921 aacacgtgat atatacccaa ttataataca tggggatgac ttttttaata aacttgacag
    10981 atggatttct gaaattggaa caggtattac acgtgatgat acaatcgtaa aagcaggtat
    11041 tgttgctgct attaaaaata atccaatgtt ttctttatta agtgaaaaca aagaatacaa
    11101 atttcttata gaaaaattat ctttattgga ggaataagat tatggaaaat ttgatggaat
    11161 atttaccact tttgattcct gttattattt tagatttaat acttataatt actgcacttg
    11221 ttcatgtatt gagacatcca aattataaaa ttggaaataa ggcaatatgg attattgtag
    11281 tactgtttat tagtctaata ggaccaatct tgtattttac aataggaaga ggtgaagaat
    11341 gatgaatatt gtctcaataa atggattatc aaaaggtttt ggtaatcgaa agataataga
    11401 taatctaaat tttactgtac cagaagggtc agtctttggg tttgtaggta agaatggtgc
    11461 aggtaagact acaacaatga aaatggtact tgggttactt gaaccagata gtggaactat
    11521 agatgtttgt ggtgagaaag tgacatatgg aagaacaagt tctaatcgtc atgtaggtta
    11581 tctgcctgac gttccagagt tttacaacta tatgagacca cttgaatatc tttctctttg
    11641 tggtgaaatt acggggcttt caaaaaaaga aataaagata aggagtgaag agttgttatc
    11701 acttgtgggg cttggcaatg aaaagagacg aataggtggg ttttctcgtg gaatgaagca
    11761 acgacttggt atagctcaag ctttgctttc acgtcccaaa ttacttattt gtgatgaacc
    11821 aacaagtgca cttgaccctg ttggtcgaaa agaaatatta gatattatgc ttaaaatcaa
    11881 agactcaaca actgtaatat tttcaacaca tatactatca gatgttgaaa gaatctgtga
    11941 ccatgttgct atactaaaca aaggaagcat tgctcttagc ggtacacttt cagaaataaa
    12001 aagtatgcac ggaaaggata gacttttact ggagtttgca agtaataatg aaattcaaaa
    12061 gtttaagtct agtggtggca ttaaatcctt actaaaagat tcagaagaaa ctaatatgga
    12121 aattgttttg catggtaaag atataaaagc aatacaaaaa acagttatat ctacactcgc
    12181 agaaatgaat ctttgtccag taaagatgga actaatagaa ttgtcacttg aaaatttgtt
    12241 tttggaggta gtaaagtgag agaatacata gcatttacaa aaaaagagtt taaggagaac
    12301 ataagaaatt ataaattact tagtttgact attttatttt tagtatttgg aataatgagt
    12361 cctctttctg caaaatttat gccagacttg attgctcatt ttgctccaac tttaaaagtt
    12421 actactgcac caactgcttt agattcttgg acacagtttt ctggaaatat ttctggtctt
    12481 ggaacgagtt taactctaat tgttttttgc aatattttat caaacgaata ttctaaagga
    12541 acactcgtta ttatgttgac taaagggtta tcacgttcat ctgtagtatt gtctaaattt
    12601 tctattgcag taattattat gacaattggt ttttggttga gctttttatg tacttatgga
    12661 tatacaatgt atttttggcc aactgcaaat cttaatcata ttatattttc agcatttaac
    12721 ttatggttaa ttagtattat gtatatttcc atattaattc ttgggtgtgt actatttaga
    12781 ccagcatttg caagtgtgtt acttgtatta gcagcaactg ctattttgag tttgatttct
    12841 attcctaaac agattgcacc ttatacgcct aatttcatat tatcaaaaaa tatagattta
    12901 gtttcaggta aagtgacacc tccagaattt attattccaa tcattgtaac aattgttatt
    12961 tcgatagtat gtctgttatt agctgttgtt ttgtttaata aaaaagcagt ttaaggatat
    13021 ttttattata aatacaaata gtgctgtatt gtgggaaaat gtgtttagta ttgtagaaga
    13081 atatatttaa gtaaaaaaga ggctgtctca agatagagaa ataagcctct tttttatttt
    13141 agtcctcctc actatacttt ataataatta agttattacc ttaaggaact atctaaaaaa
    13201 agagatatag attataatct aatgcttatc agttatttga ggtgtttagt atatagagtc
    13261 taaagtagtg aaggactaaa ataatattgt tttcattaaa taaagataag aatctttaat
    13321 tgtggtaaaa tatactaaaa aatcatttta aaagtgataa aattatttta agtaaatatt
    13381 ttaaaggggt gtagatatgg aaaagtcaaa agtatatttt tgtgatttat actcaaattc
    13441 gcaaaataaa aacgtaccaa acaatgtaag aagattattt gatgaagcag gattcaaaga
    13501 tttaatagaa aagaatgacc aagtcgcaat taaacttcac tttggagaaa agggaaatac
    13561 tacatatatg agtcctgtgg cagttagaca agtggttgat aaggttaaag actgcgaagg
    13621 aaagccattt ttaacagata caaatacact gtatacaggg agtagaacaa attctgtaga
    13681 ccatcttaca actgctatag aaaatggatt tgcttatgca gttgtaaatg caccagtaat
    13741 aattgcagat ggattatata gtagaaatta tgagaatgta aaaatagaca aaaaacattt
    13801 tgaaagtgta aaaataggtg gagaaattta taattcttct gcaatgatag taatgagtca
    13861 ttttaaaggt catgaagcag caggttttgg aggagcatta aaaaacttag ctatgggatg
    13921 tgcaagtgca gcaggaaaac aaatgcaaca ttctgatgtt actccagtag taaaggaaaa
    13981 gaagtgtgta ggttgtggaa agtgtgttaa ttcttgtcct acaaaagcta taagtatagt
    14041 ggataaaaaa gcagttatag attctgatgt atgttatgga tgtggagaat gtccaactgt
    14101 ctgtccaact agagcagtaa ctatacaatg ggaaagtgac tcagatgtgt ttgtcgaaaa
    14161 gatggcagaa tacgcttatg gagcagtatc aaataaaaaa gacaaggtag ggtatataac
    14221 ttttgttatg aatgtaactc cactttgtga ttgtgtacct tggtcaggta gaccaattgc
    14281 ccatgatata ggaatattag catcaacaga ccctgtagct attgaacaag catgctatga
    14341 tttgatttgt aaggaaatgg gtcatgatgt tttcaaacat gaacatcctc atgtaaatgg
    14401 aactagaata atagactatg catgtgaaat gggtatggga agtaaagaat atgaattaat
    14461 aaaattataa gaatagtagc acttaaatgc atataattat tgtaagagaa atctaaaagt
    14521 atcaaaggag tatgcattta tgaaaattta ctatgagaag gaaaacagca agaaggctaa
    14581 tgtaaacaat attccaagtg tatttgcgtt taattttcta agaaactttt tgactagcaa
    14641 agagtatgag gaaactagaa aagaatattt tataaataat ctaacaaaaa gtcaggttaa
    14701 tcaagccatg aaggatttaa aatggctatt tagagaatat gaaggcttag aggttattac
    14761 aatggaaagc ttggatggag ttaagacaag gatagtttta taaaccagta agaattatat
    14821 tctaataaaa aatactgctt taataataat atgtataatc tatatactaa tattattttg
    14881 aagcagtatt tttttgtgta tagtaaactt gtgtctaaaa taaagttgac caataaatgt
    14941 tatatatgca atactatata taataagtaa actacataaa aatattaagt tgacaaatag
    15001 gaggatatat ggaaaaatac ataataaaat taaaaaataa agtattgaga gaaaaagaaa
    15061 taagttatga agaagctctt aatttaataa gccttgatac aaataataaa aacgattttg
    15121 atattctatt aaaatctgca aatgaaataa gagaatattt tatgggaaga aaagcagatt
    15181 tatgtacgat aatgaatgct aaatctggca aatgtagtga agactgtaaa ttttgtgctc
    15241 aatcatctca ttataaaact ggtgtagaag aatattcatt attagattac aatgaaatct
    15301 taaatagagc taaagagatg gagagtaaag gagtgcatag attctcattg gttacaagtg
    15361 gtaaaggtat gtcaggaaag gagtttaata atattttaaa tatctatgaa ggattgaggg
    15421 aaaatactaa tttaaaatta tgtgcatctt taggtattat agattatgag caagctaaaa
    15481 tgttgaagtc agcaggtgtg actacatatc accacaatgt tgagacgtgt agagataatt
    15541 ttcataatat atgtacaaca cacacatata aagataggat aaaaactata aaagatgcta
    15601 aaaaagctgg gctagatgta tgtgtaggcg gtataattgg aatgaatgag agtgaagaac
    15661 agagactaaa aatggttttt gaaatacgag aattaaatgt aaaatcattt ccaataaaca
    15721 ttttaaatcc tattaagaat acgccaatgg aaaattatga tgtattagaa ccaatggaaa
    15781 tactaaaaac aacagcagta tttaggttta taattcctaa tgtgtacata aggtatgctg
    15841 gtggaaggct atcattaaag ggatatgaca aagttggatt taatggggga gtaaattctg
    15901 caattgttgg tgattatttg acaactgttg gaagtgggat tgaaaatgat aaaaaaatga
    15961 ttatagagca aggatttgaa ctttaataaa tactacttaa attaattaat atacttttag
    16021 ataattataa aagtatatta attttttttg cagattaatt taaatattaa aattgaatta
    16081 taagttgcaa tattttgaaa aaagggaaaa atatcaaaat ttatataaaa tggttgcaaa
    16141 tgggaaaagg atgtgatagt atctaggtaa ccggttaagt aaaacggttt cctaaatttt
    16201 gggggtgaac ttcttgagta taactataaa agaaattggc gaattggctg gtgtttctaa
    16261 aacaactgtg tcaaaagtca ttaataataa agatgaaaat ataagtcagg caacaagaga
    16321 aaaaattttg aaaataatga aggaaaaaaa ttatgtacca aataaattag cacaaagttt
    16381 agtaactaaa aaaactaata ctatagggct tcttatacca gatatacgaa atccattctt
    16441 tacagatgta tcaagaggtg tagaagacaa agctaatgaa gaaggctaca atataatttt
    16501 atgtaataca gatgaagatg ctaaaaaaga atatgaagga ataagaacac tatcagagag
    16561 gatgatagat ggaattatat ttgcagcatc ttcaaataca aactggaaag aagcaaatta
    16621 taaagatata aaaataccaa ctgtcttgat tgataagaag ataagtatga acaaagaaac
    16681 cttaaaagga attgttaaaa taaataactt tgaaggggcc tatatagcta caaaacattt
    16741 attagatatt ggaaataaga aaataatata cctaagtggt ccacttcaaa atgaaatagc
    16801 agtagataga ttagaaggat ataaaaaggc tctaatcgag tgtaatttaa gctacaattc
    16861 tgcttatgta tttgaaggaa aatataagat tgaatgggga caagaattta taaaaaattt
    16921 agaaaagata gattttgatg caatattttg tgcaaatgat ttgattgcaa taggtgtcat
    16981 aagaggtctt aaagaaagag ggttaagtat accaaatgat gtaagtgtcg tgggatttga
    17041 tgatattcaa acatcaagtc taataagccc ttctcttacc acagtaaaac agccgtctta
    17101 tgatatagga tataaagcca gtgaaatatt gataaattgt ttaagaggag ataaaaaaga
    17161 atcatttgat gaattgattt tcaaaccaga attagtcata agagattcaa caaaagaaag
    17221 tgaataacag cacgagagac ataaatagat atgtaaagga gtaatgtaat gggaaacata
    17281 gttgtcatag gaagtgtaaa catggacatg gtatgttctg tagataaaag accagaaaaa
    17341 ggagaaacag tattaggtaa tagttttttt acatcacctg gagggaaagg ggctaatcaa
    17401 gctatctcag cttctaaact aggagcaaat gtaaaaatga tatcatgcat aggggaagat
    17461 ggcttaggag aggagttaat aagaaatttt agaacgaata aagttgatta tagtttagta
    17521 tccagaaata atcacaaaag ttctggtgtt gctgttataa cattatgtga aaatgataat
    17581 agtattgttg ttgtaccagg gacgaatgag ctagtagata tagaattaat taaaaagaat
    17641 gaagaagaga taaagaatgc agatatagta ttgctacaat tagagattcc attaaaaaca
    17701 ataaattatg tagtgaattt ctgttttgaa aataggatta aggttttatt aaatccagca
    17761 ccagcagtaa aactaaatga agatataata gaaaaagtaa cttacttaac accaaatgaa
    17821 catgaatata agatagtttt tgacacaaat gaagggatag aagaagtatt aaaaaaatat
    17881 ccaaataaac ttgtaataac agaaggaaaa aatggagcta gattttatga tggtgaggaa
    17941 atcaagcatg tatcttgtat aagtgttgat gttcaagata ccacaggagc aggagataca
    18001 tttaatggag cattggcagt ggctataaca gaagggaaaa aattatatac agcagtagaa
    18061 tatgcagtag tagtatcagg tctttctgta actaaactag gtgcacaatc tggtatgcct
    18121 tataaagaag atgttgaaaa atatttaaat aataaataaa aatctaattt gttcaaattt
    18181 aaaactatta aaaatggagg ttaattatga gagtgagaaa aattttatct ttaggaattg
    18241 ctggaatact agcaatagga atgttaacag ggtgttcaat ggaaggacca agtaaatcag
    18301 ataataaagg tggttcagac aagaaagatt taactattgg agtatctaca ataactcttc
    18361 aacatcaatt ttttatagat attgatgagg gaataaaaga aaaagctaag gaattaggtg
    18421 tgaaagttat agtaaatgac ccagaccaag atgtagctaa gcaaacatca gcaatagaag
    18481 attttataca acaaaatgta gatggaatga tagtacttgg aactgataat tcggctatag
    18541 taccagcagt agaaggagca tttgaaaaaa tgccagtagt tacagtagat gctgtattaa
    18601 atactgaaaa tattacaagt tatgtaggaa cagttagtta tgatgctggt aaaaaattag
    18661 gggaatacac taaaaaatac atagatgaaa agttaggtgg aaagtcagaa atagcaatag
    18721 tgacagattt aaaatctcaa atacaaatgc agagaataga tggatttaaa gatgcgttaa
    18781 aaggttcagc aaatgttaag atattaaatt cacaaccagg atatgacaga gaagaatctc
    18841 ttaatacagt agaaaactta attcaatcta atccagatgt agatataata tatgcaacag
    18901 ctgaaaatag tgtactagga gctaaagctg cacttgaatc tgcaaaaaat aaggatgtta
    18961 agattgtagg atttgactta actgaagaag catcaagtgg aataacagat ggaactattc
    19021 ttgctatgat tcaacaacaa cctaaagaga tgggacgttt agctgtagaa gcagtagtaa
    19081 aagctataaa aggggaaaag gtagaaaaga atataccagt accagcttta ctttatgata
    19141 aggaaaatat aaaagacttt aagaattagt aaaataaaat gagattattt gaaagaaatc
    19201 ctatttaaaa aggagtgtca ttatgagtaa tataattctg aagttaagta atattgctaa
    19261 agaatttcct ggtgtcagag cattagataa tgttaatttt gagttatttc atggcgaagt
    19321 tcatgcacta ctgggtgaaa atggtgctgg taaatcaaca atgataaaga ttttaactgg
    19381 tgcacattcg aaaacttctg gaaaatttat ttttgaaggc aaagaaatag agaatatatc
    19441 cccagatatc tctaaaaaga taggtataaa tgccatatat caagaactaa cagtttttga
    19501 cgaattaaca gtagcacaaa atatttttat gggaaaagaa ataaatggca aagtcttaac
    19561 caatgacaaa aaaatgaatg aagaggctaa aaagattttc gataatatgg gaatagatat
    19621 aaatccaaat agtttagtca aagagcttag tatagctcaa aaacaaatgg tagaaatagc
    19681 aagagtttta tcatcagaaa ctaaagtatt aataatggat gaacctactt cttcaataag
    19741 taaaaaagag actgaaatac tttttagact tataaatgat ttaaaagaga gtggagttag
    19801 tataatatat atatcccata ggatggaaga actctttgag atttgtgaca gaataactat
    19861 aatgcgagat gggaaaacga tttcgacttt gaataccaaa gatgtatcgt ctgaggaaga
    19921 attagttaat ttaatgatag atagaaagct agaccaattt ttcccaaaaa gaaaagtaga
    19981 aataaaagaa gaaattatgc gagtggaaaa tctaactaag aacaatgttt ttaatgatat
    20041 tagctttaat ataagaaaag gtgaaatatt gggaataggt ggactagttg gaagtaagag
    20101 aagtgaaata gtagaagcta tatttggact tagaacatat gattctggga aaatatattt
    20161 aaacaatgaa gaagtaaagt ttaaaactcc atcagatgca atagaaaatg gactgggatt
    20221 aataacagaa gatagaaaag gtactggatt gtttttacaa atgagtgtca aagaaaacac
    20281 aactatggct gggttaaaaa agatatctaa gtttaaatca attatagata gaaaaaagga
    20341 aaaagagatt ttagaaaaat acatagaagc tttaaagata aaaacaccac atatgaatca
    20401 agttatacaa agtttaagtg gtggaaacca acaaaaagct ataatagcaa gatggctttt
    20461 attacaacca gatatattga ttatggatga accaaccaga ggtatagatg taaatgctaa
    20521 ggcagagata tacaatctaa tgggagattt agtggagagt ggtgtaagta taataatgat
    20581 atcttctgaa ataccagaat taatatcaat gagtgacaga ataatggtta tgagagaagg
    20641 tcatataagt ggatttttag aaggtgaaga aatggttgaa aataatgtat taaaattggc
    20701 tttcggaggt aaaattaatg agttcaataa ctaagcataa agaagaaaaa tcttctccat
    20761 tattgacaaa attaacaagt gtatttagag accaagggag tgcagctgta ggacttgtaa
    20821 taatttttat aataatgagt gtagcttcat ctaacttttt aactttagat aatttaatta
    20881 atgttggaag acaaatatca ataaatgcaa tacttgcagt aggtatgaca tttgtaataa
    20941 ttacaggagg aatagattta agtgtaggag cagtaattgc tttagtaggt acattttggg
    21001 ctacaacagt agtgaattat aatgcaccta tatgggtagg tatgatactt gcattagcaa
    21061 tagggacaat attaggagtc ataaaaggtg caattatttc aacacaaaaa ctacctccat
    21121 tcattgtaac tttagccatg ttaacgataa taagtggggc aagctttgta tttacaggag
    21181 gaagacctat atcagtaaat acagatgcat ttaagatgct cggtagagga tatattggac
    21241 caataccaat accagttata attatgatta tagttgtaat agcaggacat tttctattaa
    21301 aaagaactaa ttttggacgt catgtacatg cagtaggtgg aaatgaagag gccgctagat
    21361 tatgtggagt aaaagtaaat aaagttatag taaaagtata cgcactagct ggactactaa
    21421 cagcattagc tgggataata ctatcatcac gtttggcatc aggttcacca actgttggag
    21481 atggagcaga attggatgct atagcagccg ttgtgcttgg tggtactaat atgatgggtg
    21541 gttctggttc tatagtaggt acatgtatag gtgtcggaat aataggaata ttaggaaatg
    21601 gattaaactt attaagtgta tcttcatata atcaaatgat tataaaaggt ttggtaatgt
    21661 tatttgctat atggataaat aatataaaat taaaaaaatc agctaaatct aaatagtaat
    21721 taaattttag cttataaact ataaaaatta ttaagttttt gtatatattt ttatacttta
    21781 atttgcatac atctttatac attgaatggg aatatgctaa gtgaaaaagt ttactatggc
    21841 ttaaatagat gagattagac ttgaaatttt aaatgtaaaa ataaatctta ggaggaaata
    21901 tgaaaaaaat tctcttagat acattaaata gtaaaaaaca agaatacata gattatttaa
    21961 aagaacttgt aagtataaag actgaagatg ttggtcatgg aatactaggt ggatttgaaa
    22021 aagaaggaca ggaatatatt gaaaaacttg caaattacat aggttttagt gtagatagac
    22081 aagaaatgag cgaagaatta attaaaaaag caaaaaatat atataaagaa ggaaatctag
    22141 gtcacaatta tcaagataga tataatttaa tttgtaagta tagtgatgat ttacctggta
    22201 agacaattgt atttaatggt catgtagaca ctatgccacc aggtgatata agtaagtgga
    22261 aatataatcc atatcgtgct acagaagata atggaaaact ttatggtctt ggaactgctg
    22321 atatgaaatc tggacttata gcatcaatat tagctgtaaa gcttataaaa gatagtggtc
    22381 taaatgtacc tggtaatgta aaaataatgt cagtagtaga tgaagaaggt ggcggaaatg
    22441 gtaccataaa tgcagttatg aatggtattg gtggagattg ttgcataatt tgtgaaccat
    22501 cagaacaaaa cttaatagtt gcacatatgg gatttgtatt ctttgaagtt gaggttaaag
    22561 gtgtgtctct tcactgtggt agtaagtggg agggtgtaaa tgcaattgag aaggctatgt
    22621 tgttgcttca agatataaaa gagttggaac ataactggct tatgatatat aagcatccac
    22681 tattaccttc acctacaata aatttaggtg taataaatgg tggaacagca ggttctactg
    22741 tgccagataa gtgtgtattt aatttatgtg tacacttctt gccaaatata atgagttatg
    22801 aacaagttgt aaatgatgta acaaatgtca taatgacaag ggctaatgga gatttatggc
    22861 ttaaagataa taaaccaaat ataaacatat atcaatcagg gttaggtttt cagatggaca
    22921 aggattctga ttttgtagtg aatgcacata aaatactaga agaaacttta ggaaaaaaat
    22981 tagagataaa aggttcaaca gctggcaatg atgcgagatt aatgaaaaat ttagctgaaa
    23041 taccaacact tatattaggg ccaggttcaa tagaacaatg tcattcaatt gatgaatatg
    23101 ttgaaattaa ggagtattta gatagtatac ttatgtatgc aagtctaata ttaaacttat
    23161 aaaagtagga ggaatatata aatgaaaaaa gtattaatag taggggaatc ttgggtaaaa
    23221 aatataactc atataaaagg atttgatacg tttgttacaa ctcattatga agaagctgta
    23281 aagtggttga aagaagcaat agaatctgga ggatatgaga cagttcatat gcctgcacat
    23341 gtagcagcag atagttttcc gtataaatta gaagagttaa atgagtatga ctgtataata
    23401 ttatctgata taggttctaa tacgttctta ttatctaata gtacttttat agattgcaat
    23461 agtaatccag atagacttga actaatcaaa gagtatgtaa ataatggtgg ggcgttaatc
    23521 atggtaggtg gatatatgtc atttacagga atagatgcaa aagctagatt tggagaaact
    23581 gctatcaaag atgtattacc aataacaatg atagataaag atgatagagt tgaaaaacca
    23641 gcaggaataa ttccagaagt tattgactct gaacatccag tattaaaagg tataccaaca
    23701 gaatggccaa agtttttagg atataacaaa acagtagcta gagataattg tcctgtttta
    23761 gcaactattg gaggagaccc atttgtagct gttggcgagt ttggaaaagg aaaatctgct
    23821 atctttagtt ctgactgtgc acctcactgg ggtccaaaag aatttactga ttggaaatac
    23881 tataataaat tatgggttaa tatgcttgat tggttaacat gctaaaaatt ttataaaact
    23941 tgaaaacctc ttacttgata aaatgaaact tacaagtcaa gaggttttat tgtataaaat
    24001 ggttgtatgg tagaaaatat ttttaaatca gttaaaatta gtcattttgg ctgataatcg
    24061 ataagttatt agaaacaggg aggtttatat aattaatatg gataatttaa ataaaaagtt
    24121 tttgatggaa tatatagatg agatacaggg agaattaaga gatatatcag ataagatatg
    24181 gaaaaatcca gaacttcagt ataaagaata ctatgcttca aatttacaaa aagaatgttt
    24241 aaaaaaacat ggttttagta tagagggatt agaaggtatg gatacagcat ttgtggctag
    24301 ttatggagaa ggtagaccag ttatagcagt tttgggagaa tatgatgctt tagatggttt
    24361 gtctcaaaaa gtatccacta ctaaagaacc tattgtagat ggtggtgcag gtcatggatg
    24421 tggacataac ttacttggta caggttcaat aggagctgta attgcagtaa aggaattaat
    24481 caaactaaaa aagctgaaag ggactataaa atactatgga tgtccagcag aagaagattt
    24541 atctggaaaa gtattgatga taaaaaaagg tttttttgat ggaattgatt gtgcttttag
    24601 ttggcatcca tttgatataa atactccgat aagaatccca acacttgcta attactctgt
    24661 gaaatttaga tataatggga ttagtgctca tgcagcacaa gctccttata acggaagaag
    24721 tgcacttgat gcagtagaac ttatgaatat aggttgtaat tatttgagag aacatatatt
    24781 tgataattgt agagtacact atgtaactac taatggtgga aaaatgccaa atatagttcc
    24841 tgattttgca gaagtgtggt attacataag aggagttaaa atggagcatg taagagatgt
    24901 atttggtagg attgttgata tagcaaaagg agcagcatta atgactggta ctattatgga
    24961 atatgacatt ataagtggag tatatgacta tattccaaat acaatattaa cagatattct
    25021 atcacaaaat atgaaattag taggtgtgca aaactataat gaagatgact actattttgc
    25081 agataaatta gctgagacag tgtctataga taaaagaagt agtgtttcta gtgtattaag
    25141 tggaaataag gatattacta agatgaattt acatgatgag gttacagatg atacatttac
    25201 tcataataac tgtatatcat tatcattgga tattggagat gttagctaca taattcctac
    25261 agcacaatgt tcttgttcag tttggcctat tggtatttct gcccatacat ggcaatcttg
    25321 tgccagtgca ggttctgata tgggatttaa ggcaatgtta ttggcatcta aatctatatc
    25381 atgttcaata aatgatgtaa tgcttgatga attggttata aataaagcaa aaaaagaatt
    25441 gaaggatact gtaggtagtt ttgaatatac tccaattatt tgaagagtaa tttaatacaa
    25501 gtaagattga attgtatgaa ttaggatgaa tatataaatt aatattattt ttaacttaac
    25561 tttctaatta ttttcaatct aagatacaat aattagaata tatttttaaa gtttcctatt
    25621 taaggaaaat aatttgattt tatgtttgat aaaaggactt ataatttgta agccctttta
    25681 ttatatttat tttttgttta taattaattt tattttttag gtttagttag gttatagatt
    25741 accctgaaaa ataacattta acttattatt aaaaacatgt aagaatagtt tataaatatt
    25801 ttattttcga tgttgtttat tatatccatt ttatcgatgg atgaggaatg gctctgcctt
    25861 ccctagttac agatgtagta gggaaaccaa ttgcacaggc atcaaatcca tattcacctg
    25921 ttggtactcc taaaatatca caaaaatctt tattatcgct caatcttcct attacactaa
    25981 caagttcaaa acctaatcct aaagcagttg atttaagcca catattctgc atcacatgag
    26041 ataatgactt ttgttctata ggtggtattc cacgacgttc tgccacaata ataagataag
    26101 gtgctgttcc tataccaaga accccttttt caacttgcat ttttaaggaa gtaatgaagg
    26161 attgaagggt aatgttgttc tttgccttct tctcattgac tgatatttga gttttaataa
    26221 catcaacaag aatggttttt attttattca tttgtaaact gttatttgaa ataacaaaga
    26281 aatgtctgaa atctgtttgt tttgctactg aaagtattgc ataaggtgca agtcttccag
    26341 tttcaattat ttcttcaatc agcccttttg aaggtacttc ttttgtaaaa cttcttatgg
    26401 atcttctagt tcttaatagc atatccaaaa cttcattaac attgttttta tctaaattgt
    26461 tcatttttat cctccactaa tagtattctt aagatgttct ataatgcatc catataaagg
    26521 cgaacttttc ggtcatatta aacatttcct tcaaattaag tcgctatttc tatgaacaca
    26581 atgttgatta ataatctata tgtatattat aatatttata ttgcattatg caataaacaa
    26641 taatatttaa actgttaagt aataaacatt tactataaaa ttgttaatta aaattaaaaa
    26701 aatttatatg gaatgaggaa ataattcatg aaaaaagtat ctattgatag aagaactata
    26761 cgtactaaaa aaatgattag gtctgcatta gcagagttaa tagatgagaa aggatttaac
    26821 aatatatcag ttactgattt aactcaaagg gcagatataa atagaggaac cttctatctg
    26881 cattatgttg ataagtatga cttgttagag aggattgaaa atgaaataat acaggaaatt
    26941 gaagaggaga ccaaacatct tgattcatcc aacatgatgg agatagatac ctcaagtgaa
    27001 cctcttccat ttatggtaaa gctttttgaa tattttagaa aaaattcagt aattattaaa
    27061 tctatcttgg gtcccaaagg tgaccctact tttgaaagaa aaataaaaaa atttattgaa
    27121 accaatttgt ttgagaaaca aaaactaata aattttaaca tagatgaggc aataatttca
    27181 gaagaatatt ttatacaata tatactttca gcagacttag gtgtaattca atattggctt
    27241 gaaaaaaata tgaaagagtc ccctgaagaa atggcactga tacttgctag aatgtcgtta
    27301 ctcggtccgt taagagcagt gggaattcgt aaagattcat aaaaatagtt ttatatatat
    27361 tagactttga aaatatatgt ttaaaaacca ttttttacta ctactgttta taaattaggg
    27421 gagatatctt aaaatattag taaaaggagt gctaagtgag tgtaataact atgagataag
    27481 aggtttagtt gatatagtca aaacttcaaa agaatcaatt agaaaagtta aaatattgca
    27541 attaaattta aaggagtaat atatgaaaaa tgaaaatata agttatttgg cgttaggatt
    27601 atgttttggt gttgttttgg gttcattatt taaaaactta gctattggtt tatcctttgg
    27661 attagttatt ggagtagtat tagacagtaa gaaaaataaa taaaacttca atagtaacat
    27721 ataacaatta tactattttt gtataaaatc attggaaatc aacaatagaa aatatttgtt
    27781 atttaaaaac taaaaataca gtttaaacga cctaaaaata tattatataa ttattataac
    27841 aagataagtg agagtaaggt tatgataata gagtaatatt tttcaattat tactctattt
    27901 atatttattt agttatacta tagtttatat tgttatttta ttttcatatg cagtataggg
    27961 aatggattac cttgttcatc gaactcagat tttttaaaga ctttaaatcc catatgttta
    28021 taaaagccta atgcttgttg attttgctca ttcacatcta cataatttac atttaatact
    28081 tctattgcat attctacaag tctttttcca attccatttc ctatactata attacttaca
    28141 aataacattt caattttaaa atcatgtatt cccataaaag cttttataat accttttttt
    28201 gtctcttaca cataaaaaat tactaacata tttagcacat tctttaactt gaggctttat
    28261 tgatattata ttttcttcac ttaaaaagtc atgtgttgct cttactgatg attcccgtgt
    28321 atctagtatt tctttcatat ctttattgtt aataatattt aaattttcaa tttttatcat
    28381 ttaataattc ctcttaagta agtatgttac ttaatattat atgttaaata tgatttaaaa
    28441 aggttataag aatatttttg tattaaatat aatcaaataa atatatttat ttgtcataaa
    28501 ttaaatgtcc actaaaatag aaaaaataag aagaaatagg taataaatga tagcttatag
    28561 caaaataaag atgtaagtat aacaatagat gagattggaa ttaagtcact atacctaaca
    28621 gaacattctt aggcgaatat agaagactta aaaaagaaaa gagaagtaga caagaattta
    28681 ttaatgatta catataaaat aaatgaatta taattttatt aatgatataa taataattaa
    28741 caaaaaataa aagaggaacg agttaatgtt tgatggcata cactcatttt ctaaccaata
    28801 aaacaataga ttcaattaag gttactaaag tctaatcaac acataaacat ataggataaa
    28861 tacatctttt taatatatta attatgttag acttacaaaa aatgtaaata catagcttgt
    28921 taaatcttga ttgaacctta agtaataagg gataaaaaat gacttgttca aaggcaaaga
    28981 atatactatt aaatgatata agtaacttag agataatatg tagtaagtca aaagaactat
    29041 atagcatcag taagtaaaaa gcgtaaaaac ttacataatg cactataaaa taaatgagtt
    29101 agaaaataaa tataatttat ataataaagt agtacaaaaa ggagtattta tgtttggata
    29161 tgttaaaata aacaaaatgg atttaacatt tagagaatat gattattaca aagcttacta
    29221 ttgtggacta tgtaaatact taaaaagaaa tcatggagaa atctctaggt tttctttgaa
    29281 ttatgatata acatttttaa ttgttttatt aacagcagta tataacccag agtctataag
    29341 tacagaggaa gtgtgtatag taaatccatt taaaaagaaa aaggtaatta caaatgatat
    29401 tacagaatat gcagcaagta tgaatattct tttaacgtat tataaattgg aagataattt
    29461 aatggatgat aaacgcataa aagataaatt ggcatattat atatataaaa acaaattaaa
    29521 attagcatat gaaaagtatc ctgaaaaagc agagtatata aaacaacaac taaatgaatt
    29581 aaataagtta gaaaaagata aaaatataaa tatagatgaa gtatctagta tatttggaaa
    29641 tataatgggt gaagtatttg tctataaaaa agatgaaaat gagagaaact taagaatgat
    29701 aggttttaat ataggtaagt atatatatct actagatgcc tatgaagatt tagatgaaga
    29761 ttttaagaaa ggaagatata atccatttat agagtatatt gataaaaatg atgagttaaa
    29821 agaaaaagta aaaaaaataa tagtaacatc attaggattt ttagctaggg gaattgataa
    29881 tctaaatcta aataaaaatg ttggaataat agaaaatata atttattctg gtgtttattt
    29941 aagatatatt aatatattag aaagtagagg tggaaagaat gtacagtaat acttataata
    30001 atgaaaattt tatgagagca agagcattca tagaaaatag tgaatttaaa aaagcatatg
    30061 attttttaaa aactttgact gacaaatgtg cagagtggta ttatttaact ggcttttctg
    30121 caatgaacat aggatattat gaagaaggtg aagacttctt aaaaagagca aaatttatgg
    30181 agcctgaaaa tagtgaatat agtgatgcac ttagaagtta tacacaatat agaaatgatt
    30241 atagcaatag ggctgacaat tataatagaa gaagacgtaa tgatttagat ggatgttgtt
    30301 gctgttgctg tgatgattgc tgttgttgtc taggtgacga ttgttgtgaa aattgtgcta
    30361 agctttggtg tttagattca tgttgtgaat gttttggtgg agatttaata acttgttgtt
    30421 aggatgataa atatgagtat aaaagttgct tatggaggag ttttattagc attaaatgtg
    30481 atattgttga ctcttacaaa tattattcct gtaaatacat tgtttattat ggggttagca
    30541 tcacttttag tatcaattgt aataatggaa tggggcttta agagtggaat cgctttttat
    30601 ataggttcta tagttttagg atttatagtg atggcaagta agtctcaatg gattgtatat
    30661 tcacttacct ttggaatata tggtattgta aaatatctta tagaaaaaga taggtctata
    30721 tatattgagt attttatgaa attagtattt gcaaatataa tgatacttat attgtatttt
    30781 ttattaagaa caatagttta tattccaata aacatattta ttataggttc atttgagata
    30841 gcctttatag tatatgacta tgtttacagt tcttttatag gttattacaa taataggtta
    30901 agaaagatgc tatttaaaaa ataatagttg atttttatat ttaattttaa tgaaattgta
    30961 gtttgga
//
LOCUS       DAELSY010000028        30536 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.028, whole genome shotgun sequence.
ACCESSION   DAELSY010000028 DAELSY010000000
VERSION     DAELSY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.028"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(158..727)
                     /gene="yfcE"
                     /locus_tag="KPG09_001200"
     CDS             complement(158..727)
                     /gene="yfcE"
                     /locus_tag="KPG09_001200"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBG0965822.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(752..1552)
                     /locus_tag="KPG09_001201"
     CDS             complement(752..1552)
                     /locus_tag="KPG09_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBG0965823.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            1687..2559
                     /locus_tag="KPG09_001202"
     CDS             1687..2559
                     /locus_tag="KPG09_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0965824.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDREKFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVRGCSI"
     gene            2547..3233
                     /locus_tag="KPG09_001203"
     CDS             2547..3233
                     /locus_tag="KPG09_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG0965825.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            3285..3827
                     /locus_tag="KPG09_001204"
     CDS             3285..3827
                     /locus_tag="KPG09_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965826.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDIGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITESTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            3906..4373
                     /locus_tag="KPG09_001205"
     CDS             3906..4373
                     /locus_tag="KPG09_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBG0965827.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKSEKWVDVCKNW"
     gene            5081..6217
                     /locus_tag="KPG09_001206"
     CDS             5081..6217
                     /locus_tag="KPG09_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBG0965828.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLKPLASGDKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            6276..7058
                     /locus_tag="KPG09_001207"
     CDS             6276..7058
                     /locus_tag="KPG09_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG0965829.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            7077..8087
                     /locus_tag="KPG09_001208"
     CDS             7077..8087
                     /locus_tag="KPG09_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG0965830.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            8106..8903
                     /locus_tag="KPG09_001209"
     CDS             8106..8903
                     /locus_tag="KPG09_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG0965831.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            9001..9846
                     /locus_tag="KPG09_001210"
     CDS             9001..9846
                     /locus_tag="KPG09_001210"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBG0965832.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            9933..11108
                     /locus_tag="KPG09_001211"
     CDS             9933..11108
                     /locus_tag="KPG09_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG0965833.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            11303..12205
                     /locus_tag="KPG09_001212"
     CDS             11303..12205
                     /locus_tag="KPG09_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG0965834.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKKEK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            12219..13223
                     /locus_tag="KPG09_001213"
     CDS             12219..13223
                     /locus_tag="KPG09_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG0965835.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(13367..13594)
                     /gene="acpP"
                     /locus_tag="KPG09_001214"
     CDS             complement(13367..13594)
                     /gene="acpP"
                     /locus_tag="KPG09_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG0965836.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            13766..14191
                     /locus_tag="KPG09_001215"
     CDS             13766..14191
                     /locus_tag="KPG09_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965837.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            14239..14445
                     /locus_tag="KPG09_001216"
     CDS             14239..14445
                     /locus_tag="KPG09_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965838.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            14627..14806
                     /locus_tag="KPG09_001217"
     CDS             14627..14806
                     /locus_tag="KPG09_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965839.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            14840..15040
                     /locus_tag="KPG09_001218"
     CDS             14840..15040
                     /locus_tag="KPG09_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965840.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            15293..16309
                     /gene="ccpA"
                     /locus_tag="KPG09_001219"
     CDS             15293..16309
                     /gene="ccpA"
                     /locus_tag="KPG09_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBG0965841.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(16432..16830)
                     /locus_tag="KPG09_001220"
     CDS             complement(16432..16830)
                     /locus_tag="KPG09_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965842.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGGQQKKSKGLFKKTKKSNPHE"
     gene            17042..17947
                     /locus_tag="KPG09_001221"
     CDS             17042..17947
                     /locus_tag="KPG09_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965843.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            18062..18242
                     /gene="ssrS"
                     /locus_tag="KPG09_001222"
     ncRNA           18062..18242
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KPG09_001222"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            18425..19642
                     /gene="cdeC"
                     /locus_tag="KPG09_001223"
     CDS             18425..19642
                     /gene="cdeC"
                     /locus_tag="KPG09_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBG0965844.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDECNPCNPCNPCKPNPCNPCKPNPCDDKCECHDKCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSIPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(19743..21065)
                     /locus_tag="KPG09_001224"
     CDS             complement(19743..21065)
                     /locus_tag="KPG09_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG0965845.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(21116..21910)
                     /locus_tag="KPG09_001225"
     CDS             complement(21116..21910)
                     /locus_tag="KPG09_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG0965846.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQYEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            22020..23465
                     /locus_tag="KPG09_001226"
     CDS             22020..23465
                     /locus_tag="KPG09_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0965847.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSSNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTNKYKLSKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISVISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            23494..23703
                     /locus_tag="KPG09_001227"
     CDS             23494..23703
                     /locus_tag="KPG09_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBG0965848.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            23723..25117
                     /locus_tag="KPG09_001228"
     CDS             23723..25117
                     /locus_tag="KPG09_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG0965849.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSRNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTALPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNIPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGFDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            25357..26109
                     /locus_tag="KPG09_001229"
     CDS             25357..26109
                     /locus_tag="KPG09_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0965850.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDYLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            26135..26548
                     /locus_tag="KPG09_001230"
     CDS             26135..26548
                     /locus_tag="KPG09_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBG0965851.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDIECEYIILSDLYGASVCTAMLRLTNLHNVKLFSGMNLNMVLELLTR
                     FSDELTVEDIEQLVEESRRGIQSVTLQIKEEEEVF"
     gene            26552..27295
                     /locus_tag="KPG09_001231"
     CDS             26552..27295
                     /locus_tag="KPG09_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBG0965852.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLELGDMAIIGSYF
                     KDNYKDFGNVSVEHVKTFMDEIKKIREEL"
     gene            27295..27765
                     /locus_tag="KPG09_001232"
     CDS             27295..27765
                     /locus_tag="KPG09_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0965853.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDELLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            27782..28540
                     /locus_tag="KPG09_001233"
     CDS             27782..28540
                     /locus_tag="KPG09_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0965854.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            28524..29366
                     /locus_tag="KPG09_001234"
     CDS             28524..29366
                     /locus_tag="KPG09_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0965855.1"
                     /translation="MTTSSKKLDTISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSSSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGIV"
     gene            complement(29526..30431)
                     /locus_tag="KPG09_001235"
     CDS             complement(29526..30431)
                     /locus_tag="KPG09_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0965856.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKGVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
BASE COUNT        11904 a         3397 c         5407 g         9828 t
ORIGIN      
        1 atatagcata taataaaaca agtaaaccag attctattta attagaagtt taaggtattg
       61 tacttaaact tctaattgag atagaaataa aatggtttac ttgtttttat tatgagttta
      121 ataaattaaa gcttatttta tttttattaa aaatttacta atcattgata tttattatat
      181 ttccagtatt gatgtcaatt aagtttatat caagttcaag ctcatcctca tcatcagttt
      241 ttatgatatc tataattgcg acagaatggc ttccatcttt tgggatagaa gtacttcctg
      301 gatttattac tattaaatta tcatccatgt atagtttttt tacatgagta tgtcctaaaa
      361 ctaatatgtc accacccatt ttctttgctt tgtcaataat ttcttcttct gattctatat
      421 aaccatgatt caatataatt ctaatttcac caaattggct catgacataa ggactctgaa
      481 taggatgttc tataaccatc tggtcaacat ctgcatcaca gttccctttt acaatcacaa
      541 tgttttctaa tttatttaat gtctcaataa attttttagg attatatcct tctggaatat
      601 catttctagg tccatgatat aagacatctc caccatgtaa taaaacatca caatcagata
      661 atacatttaa cgctttttca aagtaaagta agctaccatg agtatcactc attattccta
      721 ttttcatatt aaaaatcctc ctaagtacag tctataaaaa tttttcagat actctatgcc
      781 aaaactcata gtttgacatt ctaagtaatt ttatttttgc atcagatata ctaactttta
      841 tattttcgat ttgtctaaat ctgtgctcaa ctccatcaac gacaatcaat acagaatctt
      901 caaatctata ttctggtgca atctttataa cagattcatt tgaacatatt atacttgatg
      961 taaagcacct atatgcattg gtatttatcg gatgtaaagg tgtaatctgc ataagttcaa
     1021 gatttatatc aacaatacta cctccagcag aatagttata ggctgttgaa cctgtggaag
     1081 tagaaataat cataccatct ccactaaaat tttgtatatg tttattatca aggcttaaat
     1141 ttaggtgtat agtcctagac ttgtctcctc ttataacaac ctcatttaca gcaagcatat
     1201 tagaaccact agtagttgta tatacttccg cattaagaag tgacatttct tgaataatat
     1261 aatctttttt tgtataagct tcaataaagc tatcaatctt atctggtaaa atatcaggaa
     1321 aaaaccccag atgacctgta tttattccca ttatgggaat ctcaggaaaa tcgaaatctc
     1381 taactgttct taaaaaagag ccatcaccac caattgatat aataagctct gtgtctggat
     1441 aaatatctga atacacttca aagccagcat tgataagttt tctagttaag agttctttag
     1501 tctcaaggga cttatttaat tggtttgtat ttatagttat aattcttttc atatattcac
     1561 ctcttcatac tttgtattat aacatagttt tataattctt aatttataaa tttataaatg
     1621 acctttgtag tgaatgtgtt aaaatatgta taggtgggta taaaaaaatg cataagaggt
     1681 gaagatgtgt ttagcataag gaatatcaca aaaaaattag gaaaattcaa gttaaataat
     1741 ataaacttag agttaaagga aggggatatt gttgggatta taggaccaaa tggttcggga
     1801 aaaacgactt taataaaaat tataatgggg attattgatg cagatgaagg tgaaattgaa
     1861 ctttgtaatg aaactattga aaattctcca ataagcttta aaaacaatat tggttttgta
     1921 tatgattctt tacaatttta tccacattta aaggttaagg aatttagaaa aatagtttct
     1981 ttattttata agaattttga tagagaaaaa tttgatgaat accttaataa gtttgacata
     2041 gaagaaaata tgcatattga aaatttatct aaaggtcaat ctgagaagct tatgttatca
     2101 agcgcactta gtcataatgc taagttattg attttggatg aaccaactgc tggtatagac
     2161 ccgattgtaa gaactgaaat aatgcagtac ttacaagatt ttgtgaaaaa tggaagtagt
     2221 agtgtaataa tttctactca taatactgat aatttaatta aaattgctga ttatcttgtt
     2281 tttataaata gaggaaatca gatttttact gttaagaaag aattaataga acaagaatat
     2341 aagataatta gggcaaataa agcagagtta gaagcaatta aagaaagtat agttggtgtt
     2401 aaagagtata aatattataa tgaggcactt gttaaagttg gagatagctt gaaagtaaaa
     2461 agcctattga tagaaattga taagcataag gtcaaaaatc ctactataga agaactaatg
     2521 tattattatg taaatgaggt gagaggatgt tcaatttaat aagaaaagat ttaataatag
     2581 gaattagttc agatggtatc cgtaatttaa agtacatatt gctatttttt gtgttttatt
     2641 ttttccttaa ttcaatttca tattatacag taagtattgt tgttagttat ttgatattta
     2701 taaatacatt tgagtgtgat tatgaaaatg attcaaggat ttttataagg agtatgccag
     2761 ttagtataga agatatagtt tactcaaaat atttattagg tgtaggacta ataatttctg
     2821 taacaataat tgtgtcctta ctaagtaaat tgacatcact agtgttcttt agaaatatgg
     2881 ttttaaatga tgtatttttt tctgtaaata tatttttagc aattttgacg attttactgc
     2941 ctttaatttt taagtttgga tatggaaaaa tgaaagtttg tggttttata gtaagtattt
     3001 taatttactt tgtatatgga agtcttttga agatgattag tatgattgta tatcaagtaa
     3061 agcattttaa ttatagtaaa gttggaggag tatatctttc aaattatgta actgatatag
     3121 ctaatactaa gtatataaat ttatatagta tgacattttt aacaataata atttttataa
     3181 tatcaatgta tttttctatt aagatttcaa aaaagaataa gtttaattat taaaatttta
     3241 attaatatta tagcacaata gcacaaaata taaggagact aaaaatgaag aagtttataa
     3301 aagtttcatt ttttattttg gggattttta cactattcat agctataaat aaattagtat
     3361 atagagatat ggtaagtgtc aaagattatg aaataaatca tttagaggaa attgtttttg
     3421 attttgatag caggagaagt agagatatcg gtactattgt aagatttaaa ataagcaata
     3481 atagtaaata tagttataaa ttaaacagtg ctaaaatgaa gtttgaaaat tgtgtggaaa
     3541 ataaaaaggg aatgaataag tgttctgcat atgtaaatgt agatttattt aatgaaaaaa
     3601 attctataac tgaaagtact ctgaaatatg gaataaaacc aaataatcaa ggttatgttg
     3661 attttgtaat tccaaaagga ctaaattttg attataggta ctttaatgaa tctggtatga
     3721 gtgtagaata taaaggagaa tatattgtca atattccaat ggcaaaagga ctttatctag
     3781 tagttggaaa aaataatgat acatggaatg ctaatattat ggaataattt gaaaaataaa
     3841 tcaattatag atataattaa gaataatatg aaatttataa aaattcagac taggaggggt
     3901 atttaatgtc aataagtaat gtaagagagt attttaaaca atttggcaag gaagacagta
     3961 tattagaatt tgaacaatct agtgctactg tggaacttgc agctgaagca gctggggtta
     4021 ttccagcaag aatagctaag acactctcat ttaaaatagg agatgatgca attttaattg
     4081 tgacagctgg tgatgctaag attgataata agaagtataa ggctgaattt aattgtaagg
     4141 ctaaaatgtt gacaccagaa gaagtcttgg agtttactgg acatgctatt ggtggagtct
     4201 gtccatttgg tttaaaaaat tctataaaag tctatttaga tgattcaatg aagagatttg
     4261 atacagtatt tcctgcatgt ggcagtagta actctgcaat agaacttaca tgtgaagaaa
     4321 tggagaagtt ttctaagagt gaaaaatggg tagatgtttg taagaattgg taaaaaaata
     4381 tagtaaacta tagtaggaat tagtaaaaaa ctacctctgt atacaatcta tacagaggta
     4441 gttttttgtg taaatattta taaatctaag aggaaaatct aatttaaact aataatccaa
     4501 aataaagtta aaaaattctg attttttttc gaaacaataa aaaaatgaca aaaaagtgga
     4561 aaaaatgtaa taaatattgt taaaaaaata attagaaatt attgaaaaat tatcctttga
     4621 gtgttactat tatagatgta agatatttaa aaataaaaaa tagtcagata aaaatacgat
     4681 tatttagctc tattaaaata atttttatac gataaaaatc ttctgtacat ggctaaaaaa
     4741 cagatgtaca gacaatattt aatgtttaat ataccctaat ataaatatcg taaacaaatt
     4801 attttatctg tgaccagtgt gaaagaaatc ttattctaac ttgaaatttc acaaaattcc
     4861 aaaaagcgag atttttttta agtatagttg attttaaatt aacaatttat gattaaatta
     4921 atgtcataat tggttagtat aatcacatat aaatatagag cgtagtttga ttaaggttat
     4981 aaatttctcg acgtatattc aaatacctta ttatagatgt tgccgagatt ttaattaata
     5041 aatgaaaata aaatatttat aaaatactga ggaggctatt atggatttaa attctaaaaa
     5101 atatcagatg cttaaagagc tatatgtaag cttcgctgaa aatgaagtta aacctttagc
     5161 aacagaactt gatgaagaag aaagatttcc ttatgaaaca gtggaaaaaa tggcaaaagc
     5221 aggaatgatg ggtataccat atccaaaaga atatggtgga gaaggtggag acactgtagg
     5281 atatataatg gcagttgaag aattgtctag agtttgtggt actacaggag ttatattatc
     5341 agctcataca tctcttggct catggcctat atatcaatat ggtaatgaag aacaaaaaca
     5401 aaaattctta aaaccactag caagtggaga taaattagga gcatttggtc ttactgagcc
     5461 taatgctggt acagatgcgt ctggccaaca aacaactgct gttttagacg gggatgaata
     5521 catacttaat ggctcaaaaa tatttataac aaatgcaata gctggtgaca tatatgtagt
     5581 aatggcaatg actgataaat ctaaggggaa caaaggaata tcagcattta tagttgaaaa
     5641 aggaactcct gggtttagct ttggagttaa agaaaagaaa atgggtataa gaggttcagc
     5701 tactagtgaa ttaatatttg aggattgcag aatacctaaa gaaaatttac ttggaaaaga
     5761 aggtcaagga tttaagatag caatgtctac tcttgatggt ggtagaattg gtatagcagc
     5821 acaagcttta ggtttagcac aaggtgctct tgatgaaact gttaaatatg taaaagaaag
     5881 agtacaattt ggtagaccat tatcaaaatt ccaaaatacc caattccaat tagctgatat
     5941 ggaagttaaa gtacaagcgg ctagacacct tgtatatcaa gcagctataa ataaagactt
     6001 aggaaaacct tatggagtag aagcagcaat ggcaaaatta tttgcagctg aaacagctat
     6061 ggaagttact acgaaagctg tacaacttca tggaggatat ggatacactc gtgactatcc
     6121 agtagaaaga atgatgagag atgctaagat aactgaaata tatgaaggaa ctagtgaagt
     6181 tcaaagaatg gttatttcag gaaaattatt aaaatagata aatattaaaa tatacacaat
     6241 aggtcgatat taaaatagat atggaggaag gatttatgaa tatagtcgtt tgtataaaac
     6301 aagttccaga tacaacagaa gttaaactag atcctaatac aggtacttta attagagatg
     6361 gagtaccaag tataataaac cctgatgata aagcaggttt agaagaagct ataaaattaa
     6421 aagaagaaat gggtgctcat gtaactgtta taacaatggg acctcctcaa gcagatatgg
     6481 ctttaaaaga agctttagca atgggtgcag atagaggtat attattaaca gatagagcat
     6541 ttgcaggtgc tgatacttgg gcaacttcat cagcattagc aggagcatta aaaaatatag
     6601 attttgatat tataatagct ggaagacagg cgatagatgg agatactgca caagttggac
     6661 ctcaaatagc tgaacattta aatcttccat caataacata tgctgaagaa ataaaaactg
     6721 aaggtgaata tgtattagta aaaagacaat ttgaagattg ttgccatgac ttaaaagtta
     6781 aaatgccatg ccttataaca actcttaaag atatgaacac accaagatac atgaaagttg
     6841 gaagaatata tgatgctttc gaaaatgatg tagtagaaac atggactgta aaagatatag
     6901 aagttgaccc ttctaattta ggtcttaaag gttctccaac tagtgtattt aaatcattta
     6961 caaaatcagt taaaccagct ggtacaatat acaatgaaga tgcgaaaaca tcagctggaa
     7021 ttatcataga taaattaaaa gagaagtata tcatataatt taagggggga taaagaatgg
     7081 gtaacgtttt agtagtaata gaacaaagag aaaatgtaat tcaaactgtt tctttagaat
     7141 tactaggaaa ggctacagaa atagcaaaag attatgatac aaaagtttct gcattacttt
     7201 taggtagtaa ggtagaaggt ttaatagata cattagcaca ctatggtgca gatgaggtaa
     7261 tagtagtaga tgatgaagct ttagcagtgt atacaactga accatataca aaagcagctt
     7321 atgaagcaat aaaagcagct gaccctatag ttgtattatt tggtgcaact tcaataggta
     7381 gagatttagc gcctagagtt tctgctagaa tacatacagg tcttactgct gactgtacag
     7441 gtcttgcagt agctgaagat acaaaattat tattaatgac aagacctgcc tttggtggaa
     7501 atataatggc aacaatagtt tgtaaagatt tcagacctca aatgtctaca gttagaccag
     7561 gggttatgaa gaaaaatgaa cctgatgaaa ctaaagaagc tgtaattaac cgtttcaagg
     7621 tagaatttaa tgatgctgat aaattagttc aagttgtaca agtaataaaa gaagctaaaa
     7681 aacaagttaa aatagaagat gctaagatat tagtttctgc tggacgtgga atgggtggaa
     7741 aagaaaactt agacatactt tatgaattag ctgaaattat aggtggagaa gtttctggtt
     7801 ctcgtgccac tatagatgca ggttggttag ataaagcaag acaagttggt caaactggta
     7861 aaactgtaag accagacctt tatatagcat gtggtatatc tggagcaata caacatatag
     7921 ctggtatgga agatgctgag tttatagttg ctataaataa aaatccagaa gctccaatat
     7981 ttaaatatgc tgatgttggt atagttggag atgttcataa agtgcttcca gaacttatca
     8041 gtcagttaag tgttgcaaaa gaaaaaggtg aagttttagc taactaattt cttggggagg
     8101 gtattatgag tacaagtgat gttaaagttt atgagaatgt agctgttgaa gtagatggaa
     8161 atatatgtac agtgaaaatg aatagaccta aagcccttaa tgcaataaat tcaaagactt
     8221 tagaagaact ttatgaagta tttgtagata ttaataatga tgaaactatt gatgttgtaa
     8281 tattgacagg ggaaggaaag gcatttgtag ctggagcaga tattgcatac atgaaagatt
     8341 tagatgctgt agctgctaaa gattttagta tcttaggagc aaaagctttt ggagaaatag
     8401 aaaatagtaa aaaagtagtg atagctgctg taaacggatt tgctttaggt ggaggatgtg
     8461 aacttgcaat ggcatgtgat ataagaattg catctgctaa agctaaattt ggtcagccag
     8521 aagtaactct tggaataact ccaggatatg gaggaactca aagacttaca agattggttg
     8581 gaatggcaaa agcaaaagaa ttaatcttta caggtcaagt tataaaagct gatgaagctg
     8641 aaaaaatagg gctagtaaat agagtcgttg agccagacat tttaatagaa gaagttgaga
     8701 aattagctaa gataatagct aaaaatgctc agcttgcagt tagatactct aaagaagcaa
     8761 tacaacttgg tgctcaaact gatataaata ctggaataga tatagaatct aatttatttg
     8821 gtctttgttt ttcaactaaa gaccaaaaag aaggaatgtc agctttcgtt gaaaagagag
     8881 aagctaactt tataaaaggg taaaaatcta attaaaaata tatttgctgt taactagatt
     8941 aaatagtatt ttaatctagt taacagaata taaatactaa aataacagga ggttataact
     9001 atgaaattag ctgtaatagg tagtggaact atgggaagtg gtattgtaca aacttttgca
     9061 agttgtggac atgatgtatg tttaaagagt agaactcaag gtgctataga taaatgttta
     9121 gctttattag ataaaaattt aactaagtta gttactaagg gaaaaatgga tgaagctaca
     9181 aaagcagaaa tattaagtca tgttagttca actactaatt atgaagattt aaaagatatg
     9241 gatttaataa tagaagcatc tgtagaagac atgaatataa agaaagatgt tttcaagtta
     9301 ctagatgaat tatgtaaaga agatactatc ttggcaacaa atacttcatc attatctata
     9361 acagaaatag cttcttctac taagagacca gataaagtta taggaatgca tttctttaat
     9421 ccagttccta tgatgaaatt agttgaagtt ataagtggtc agttaacatc aaaagttact
     9481 tttgatacag tatttgaatt atctaagagt atcaataaag taccagtaga tgtatctgaa
     9541 tctcctggat ttgtagtaaa tagaatactt atacctatga taaatgaagc tgttggtata
     9601 tatgcagatg gtgttgcaag taaagaagaa atagatgaag ctatgaaatt aggagcaaac
     9661 catccaatgg gaccactagc attaggtgat ttaatcggat tagatgttgt tttagctata
     9721 atgaacgttt tatatactga atttggagat actaaatata gacctcatcc acttttagct
     9781 aaaatggtta gagctaatca attaggaaga aaaactaaga taggattcta tgattataat
     9841 aaataagaat ttgggaatta aaagtttaaa taaaattgtt taaaaaacaa tttcggatat
     9901 atgaaaaatc taatttaatg ggggtaatga atatgagaga agtagtaatt gccagtgcag
     9961 ctagaacagc agtaggaagt tttggaggag catttaaatc agtttcagcg gtagagttag
    10021 gggtaacagc agctaaagaa gctataaaaa gagctaacat aactccagat atgatagatg
    10081 aatctctttt agggggagta cttacagcag gtcttggaca aaatatagca agacaaatag
    10141 cattaggagc aggaatacca gtagaaaaac cagctatgac tataaatata gtttgtggtt
    10201 ctggattaag atctgtttca atggcatctc aacttatagc attaggtgat gctgatataa
    10261 tgttagttgg tggagctgaa aatatgagta tgtctcctta tttagtacca agtgcgagat
    10321 atggtgcaag aatgggtgat gctgcttttg ttgattcaat gataaaagat ggattatcag
    10381 acatatttaa taactatcac atgggtatta ctgctgaaaa catagcagag caatggaata
    10441 taactagaga agaacaagat gaattagctc ttgcaagtca aaataaagct gaaaaagctc
    10501 aagctgaagg aaaatttgat gaagaaatag ttcctgttgt tataaaagga agaaaaggtg
    10561 acactgtagt agataaagat gaatatatta agcctggcac tacaatggaa aaacttgcta
    10621 agttaagacc tgcatttaaa aaagatggaa cagttactgc tggtaatgca tcaggaataa
    10681 atgatggtgc tgctatgcta gtagtaatgg ctaaagaaaa agctgaagaa ctaggaatag
    10741 agcctcttgc aactatagtt tcttatggaa cagctggtgt tgaccctaaa ataatgggat
    10801 atggaccagt tccagcaact aaaaaagctt tagaagctgc taatatgact attgaagata
    10861 tagatttagt tgaagctaat gaggcatttg ctgcccaatc tgtagctgta ataagagact
    10921 taaatataga tatgaataaa gttaatgtta atggtggagc aatagctata ggacatccaa
    10981 taggatgctc aggagcaaga atacttacta cacttttata tgaaatgaag agaagagatg
    11041 ctaaaactgg tcttgctaca ctttgtatag gtggtggaat gggaactact ttaatagtta
    11101 agagatagtt ttagattata atatttacaa attaaatatt taattatgaa tttaacctgt
    11161 atgaataact atttgttatt cttacaggtt tttatagtta aaaataatta ttatctatta
    11221 aacaaaaata gtgtaaaata tataatatca tgattttgaa gatataaaaa gcaggaagat
    11281 ataaaagaaa gtaggtaaaa gtttgtttaa aaaagagaat caaagatata atcttatatc
    11341 gtatacaaat gaagaagaaa tgactttaaa agaagtttta ttggacaagc taaatttctc
    11401 agttaggtca ttatcaaaaa tgaagaagga aaagagtgtt ttagtaaatg gagtatataa
    11461 aaaaccaagt ttaaaggtgt atagtggtga tttaatagaa gtaaaaatag acgaggaaaa
    11521 agcaaatttt gaacctcaag atttaaattt acagataatt tatgatgatt ttgatataat
    11581 tatggtgaat aaacctccat ttatggtagt tcacccaaca aaaagccact atgataaaac
    11641 aatagcaaat ggtattagtt actatataga taatcaaaaa gaaaacgtaa aaataagatt
    11701 tgtaaatagg ttagatatga atacttcagg gttagttata gtagcaaaaa atgcttatgc
    11761 acatcatact ttatctacag caatgagtga aaataaagtt gaaaaaaagt atataacagt
    11821 agttgatgga attataaaag aaaatgaggg aactatagat gagccaattt atagacctac
    11881 agaagattca ataaagagga taatagatga aagaggacag tcatctgtta ctcactataa
    11941 agtaatagaa agactagaaa atgcaactgt attagaagta agcttagaaa ctggtagaac
    12001 acatcaaata agagtacata tggctcatat aggtcatgga ataataggtg atgagttgta
    12061 tggttatgta gatgaagagc taataaatag gcaagcactt catgcataca agctagagtt
    12121 tgaacaacca agaacaaaag aaaaattgaa atttaaagca gatataccag aggatatgaa
    12181 agagttaata tcgaaattaa gataatgggg tgatatatat gataatacat aaatttataa
    12241 tacatgtttt ggacaaaaat agtgatgttc caatattaaa tgactttgaa ggcaaagtta
    12301 atcaagaagt agatggtttt ttccagaaag ctataaaaag aattgcaaaa gatgaggatt
    12361 taagaaaagg tgtttttaaa gattataatg acaatttaat taaaaactgt tgtgaacaaa
    12421 taatatatga tgaaagtact tttttgaaaa attctaaaga aattgcatct tacttgtttg
    12481 acgttatgaa aattaatgct gcactagaat cttgtgattt ggcaatttgc ttatacacta
    12541 ttaaagatga aaagagtgta gcaatactga aattagatta taaaaagttg tacactcatt
    12601 caattgaatt tgtggatgat aaatttaata ttcaaatggt ttcaaatgaa attggtatcc
    12661 cagagacttt aagacaaaag caaggagctt tgattagttt aagtggtata aatgatgagt
    12721 ttcatctaag gcttttagat aaggatgctg aaaaagaagg gtcagaatct aagtttgtaa
    12781 cagaattttt aaatgctaaa aaaatagatg atgataagta caagaccaag gtatttaaaa
    12841 atacagctga aaattggata actaatgctc ttagtaatga tataaagcaa gctgaagatg
    12901 taagaagtat attaaattat acactgaagg aaaaacatga agttgatata aatgactttg
    12961 ttgataattc aattaaagat gatgagttaa aggatagctt taaagagcat atggaagaaa
    13021 aaggtcttga tgaaagtttt agtatagata aaaaatgggt tgagaaaaag cttaaaaaaa
    13081 gaagtataaa aacggataat ggttttgaca taaaagggaa tctaactgat tttgaagacc
    13141 caatgaagta tactgtaaag caaaatcaag atggaactat agatataata ataaagaatg
    13201 ttacatttta tgaagaaaag taatttatat acttagtata taaattaaat ataagagcgt
    13261 gtattttata tattgtaaat aaatgtaatc tagtctgtgc gaactctaat ataatttatt
    13321 atgtagaagt ttacacagac tatttatatt tgtagacaaa acgatactat ttgttttctt
    13381 cgatatattt acatatatca ccaatacatt taaagttctc agcttcctcg tctggtattt
    13441 ctattccaaa ttcatcttca agggccatta taatttcaac agcatctaat gaatcagctt
    13501 ctaagtcgtc cattaaagat gcttccatag ttatctcatc taaattgtct acccctaatt
    13561 gttctgctat tatttctttt attttctcaa acattttaag cctcctaaag cagttttaat
    13621 taattttaat gttagtttaa tataatatca agaatttttc aatatggaat ctataaaaaa
    13681 taataaaatt ttataaatgt agtaataaag tattatttga tgaataaact ttattgataa
    13741 caaatataac ttaggaggaa atgctatgaa atatgtagga ctattactaa cttcagtagg
    13801 aatgttttta cttatagcag taaattttta ttataattca ataactttag atatgcaacg
    13861 tatagaggat tatgtaatgg agaccaattt aatccttgaa gatgtagctg aaaaggagag
    13921 ttatgtatct aatgaaaaag aagactacat atcaaggtta atgcatgtaa agaaaggaat
    13981 agaaaattca aaaacatcct tcttgataga aagatataag gagtacaaaa taaaatcaat
    14041 agaaagttta atctacacta tatcagagga gaaaaaagat tatctggatg aagttgatag
    14101 atacaataaa cttggtgaaa aagaaataaa taaactaatc aataaaaact ttttagaagt
    14161 aacttatctg tctattacta catatattta attaaagaga caaaacttag tctcttaatg
    14221 attcaaggag gattagttat gacaaaatta gaagacaata tggaacgttt ctttggagga
    14281 tgcgaaggat tttttggtaa taaaatgttg ataataatag ttatagtatt cttactatta
    14341 tgcacagaca ttttagaaga cttattatgc gatgacaata tatggatatg ggttatatta
    14401 atagtattgt tattatttaa cttcgatgat ggttgttgtt gctaaaaaat attgattatt
    14461 aaataggtaa aaccagtaac ttatacaagt tatggtttta ccttttttag ttcaaattaa
    14521 aaaatttaaa aaaatagtaa aaatacatta gcaaaatgga aaccaaatgc atagtatatg
    14581 ttaggagcga aaagctcaac aaaatatcaa caggaggtaa cgtacaatgt tagatagatt
    14641 ctttggagaa gaaggatgtt ttggtggagg aggatggtgg ataatagtac tattcttcct
    14701 attcttagct tttggagata gctgggcaga atttgacata atggcatgga taccattcct
    14761 aatagtttta ttaatacttt gctgctgtgg tggatgctta gaataagaac acagttatta
    14821 aaaaaaggag ggctttgtta tgttagatag attttttgga gaagaaggat gctttggtgg
    14881 aggaggatgg tggataatag tactattctt cctattctta gcttttgaag attgctggag
    14941 agacattgac ataatgtcat ggataccttt cttaatatta ttgctaataa cttgttcatg
    15001 tggtggattc tttgatggtg aagtaggttg cggctgctaa tacttaaatc attagaataa
    15061 aaaaatgctg gtattttata ccagtatttt tttattttat tttttaatat gatttaaatt
    15121 aagaatgaat attattaaat ttgttgaata tagggtataa tataaaatat aatgtattta
    15181 aatgtacttg cttttatggt aaaataaaaa cttatataaa tgtagttaaa ataggaacct
    15241 tcaaaaattt ggttcagttt tagataaatt tagtaaaata aggaggatga agatgaaagg
    15301 caatataacg ataaaagatg ttgctaaaca agcaggagtg tcaatatcta ctgtatctag
    15361 agttataaat gattcaaaac ctgtaactga tgaagtcaaa caaaaagttt tagaggttat
    15421 aaaagagact ggatatatac caaatccact tgctagaagc ttagtaacaa agaagagtca
    15481 attaataggg gtaatagttc cagaagtttc agattctttt gttaatgagg tgttaaatgg
    15541 gatagaagaa gttgctaaaa tgtatgacta tgatattctt ttagcgaata catactctga
    15601 taaggaacaa gaacttaaga gtataaatct attgagagca aaacaagtgg aaggtatagt
    15661 tatgatttca tggatagttg aacaagaaca tatcaactat atacaaaatt gtggaatacc
    15721 agcgacatat ataagtaaaa ctgctagaaa ttatgatata tatacagtaa gtactagcaa
    15781 cgaagaagct acttttgata tgacagagca tcttataaag aaaggtcatg aaaagatagc
    15841 ttttataatg acgagtaaag atgatactgt tttagaaatg gaaagacttg ctggttatga
    15901 gaaagcactt tcaaataaca atatagaatt agacaagagt ttgattaagt atggtggaac
    15961 tgattatgag agtggataca atagtatgaa agaactatta gatgatggaa taatacctca
    16021 tgcggctttt gtaacaggtg atgaggctgc cataggtgct ataaatgcta tatgtgatgc
    16081 tggatataag gttccagaag acatatctgt tgcaggattt aatgatgtta agatagctag
    16141 aatgtataga cctaaactta ctacagtata tcaacctcta tacgatatgg gagcagtagc
    16201 aataagaatg gttataaaat taataaataa ggaattaatt gaaaataaga aaatagaatt
    16261 accttataga attgttgata gagaaagtgt tacagaaaga aaaaaataaa actattaaaa
    16321 tcaatcaggt agtgcctaag gcactacctg attttttatg tcaataatat caaatttaat
    16381 aatagattaa ctaaaaataa ttatctaatt acttaaatag tttattttta tctactcatg
    16441 agggttagac tttttagttt tcttaaataa acctttagat tttttttgtt gaccacctgg
    16501 tgtatttttt ggtgcaggtg aatttgattt acctttagta ggtttagcct tcttagtttt
    16561 agctggttta gtatctcttt gattttctgc atctgagatt ttttccatta tattatctag
    16621 ttttttgcgt tgattttcat ctaaaaatgc tttgaatttt gatactactt catctcttcc
    16681 atctaagttc ttacctattt ttataagttc ctccataagt tcattttctg attttccttt
    16741 gtaactatca gccatctttt ctattttatc cttgtcaata tttttttgtt tagccatttt
    16801 ttctaatgac tctgtatcta attttttcat aaaaaaaccc ctttctataa atatatattg
    16861 aattatattc atactattta tatatagaaa taatattgaa gtggtgaatt gtgagtgata
    16921 tataatataa ataaaaattc ttatagataa tatatatgca ctgagcttta aaaatggaac
    16981 attaaaatat gaaaaacata ctttaatata gggcaattat ataatataag ggggaattgt
    17041 cttgaataac ttactatttt tgattggagt gattgcattg agtaatggta atatctctat
    17101 atttgatgat aaaaataaat caaaaggaaa taatagagtt agaaaaaaca aaaaaaatgc
    17161 aaatgataaa ctttcacagt ctacacatgg caatttaagg tttgatgcaa atgatataaa
    17221 aagaggttta aggctcatgg atttaagtga agaagattta gaaatgggaa tggaaataat
    17281 aacaagaact aaaaaataca tgtcaagaga tgaaagaaaa atactaataa aaatagaaag
    17341 tgttttagat ttagttagag gaataaaaaa attaaataat atagatatgg tagatacaga
    17401 agatgaaacg gattttttca gaaaaatgga tagtgatgat aaaaagaata tgatgataaa
    17461 ggaaattata gatgtattcc cagaaaaaag aaaaaattca gttgaaaaag ctataggaat
    17521 gaaaaagaag atagatttat ttgcagaatt atttttacca gatgactttg gtgaaggtgg
    17581 atttagtttg agctctttgg caaatataaa caatcttggt agtatgaata atctcaaatt
    17641 gttaggaagt cttttaagag gtgatgatag taataatgat gatgaagaca ataatgatga
    17701 gttaaattat gaagacgaag aattagattg tgaagacaaa gaattggatg atgatgagtt
    17761 aacagatgaa ggtgaaatgg aggataatga agatgaatta gtagaagata agaacacagg
    17821 ctatgaaaat atagaaaatg atgaagatat agtatacgat gaggaactat acaacattaa
    17881 tgagcaagaa tccgaataca attataatga aaaaaatact aaaactagag ctaagaaaaa
    17941 acgataatta actaaataca taaagaaaaa aggaagaaaa agtaatgata gaataagatt
    18001 atctcaatta ctatataaaa ccactttaaa aaattcaaaa tttattatat aataataata
    18061 tgaaatcctg aaacatacga taaggctttt ttaaattcaa cctacaaatg aaatacggga
    18121 gaccgtgtct ggtaaattat aacatgccta gttagtttgg acgttaaagg ttactggcaa
    18181 gtcacccacc tgggagtgcc tagggtgtga atttacttag ccaccggtgt ttcgggacaa
    18241 aaaaagtgct actatttcaa atagtagtac tttttttatt aatatgcatt tttctactaa
    18301 atacgcctaa ttattgataa attcaaaaag caagtaacaa ttaagattat aaaagcatag
    18361 tattaattag gcttcaaaaa gccaaagact taataagaat cgcttacagg gagggcaatg
    18421 aaaaatgcaa gattataaaa aaaataaaag aagaatgatg aatcagccaa tgtctacaat
    18481 gaatgaagaa gaagtgtata cagatgaaat aaattcagaa gacatgagag ggtttaaaaa
    18541 atcacaccat cataatggat gtaatactga taataagtgt gagtgccatg atgagtgtaa
    18601 tccatgtaac ccatgtaatc catgtaaacc taacccatgc aatccatgca aacctaatcc
    18661 atgtgatgac aaatgtgaat gccatgacaa gtgtaaatgt gattgtgaac catgtgaaat
    18721 ggattcagat gaatgttttg aaaacaaatg tggaccagaa tgttgtaatc cgatatctcc
    18781 aagaaacttc tctgtatcaa atgcagtgcc atttgcaata gaggctaata gaatatttga
    18841 tactatgcaa ttccaaacat ttacagatgc aacaggtcca aatggagaac cattaacttt
    18901 tgaaacagaa gtagtagaag tatttggttc aattccaagt gcaggtcaag caagtgtaac
    18961 tatagaaaaa atatgcttaa gtaatgatgg aatagttata gacacaggaa tgacaacttt
    19021 agaagatttc gatttagacc cattaggaga tatagtagga agaaactgtg aaacaacttt
    19081 tgaatttgca gtttgtggag aaagaaacgc tgagtgctgt agacaaggaa aaggcaaatc
    19141 agtagcttat aaacaaagag gattaactgt agcagttcgt aatttagtac tagagctaag
    19201 aggtagatgt ggatgtacag agttcgttgc attagctttc ccagcagtta gagcaggagg
    19261 tggatgtaag agaagagttg attatgtaga atttactttt aacacacttt cagcaccaat
    19321 atgcttgcca gctgacggaa gagctgttac tttaagacaa gaatatcaaa ctaacttaac
    19381 tgtagattgt ataggaaaat ctatattaaa attagaatgc aacgaatgtt gtgaaccttt
    19441 ctatgaatta attataccaa atgatataga tttagtactt tgcttacaag aaacagttag
    19501 cacattaata agtgaacaaa tagtagtttt agcatcacca aatccaatcc aaccaagact
    19561 tgttgatact ttctctaaag tatgtgattt ttcgcaatgt ggacctaatc atggaagtgg
    19621 aaagccaagt tgccacagat agataggtaa gatatataga cagtttaaat tataaataaa
    19681 aatcatctaa ggaaggctac caaagtgtat aatttacatt ggtagccttc acgtttttat
    19741 aactagcgta tagattttag tgatttaaaa agcactatag tatgtaatag acaagtaaag
    19801 attgatacta tcgtcattgt gtatatgtat gcataaatat taagtcctgg aattggaaga
    19861 aatacatata aagctattag ttgaattaac atgccagtaa tagtatttat acttgaagct
    19921 aattcttttc ttattgaatg taaaatgcca gatagagttt gatttaggga cataaatagt
    19981 ggaactaaaa acatagcttt tagatattct cctgcaagtg tatttttaaa tactaatata
    20041 cataaatctt ttccaaagaa ataaaaaaat attgatgata gtatgcctac aaataaagta
    20101 agaagtatgg agtatttgat ttttttaatt acactcttac gctttcttag agccatttcc
    20161 tgtgaaatag ttggtattaa atttacaata agcgctgaac caacagtaaa aggtaaaaat
    20221 ataaagggca ttaccatccc actgacaata ccatacatac ttaaagattg ttgataagtc
    20281 attcctgata atgctaatct tgatggtatc atcatagaac ttatagattg caaaagtatg
    20341 tttgacattc tattacaagt tatgggaata gacattttta atgtttccat agaagaatta
    20401 taaaaatctt ttatgcttat aatataccta ttgtcaaatg atgaatctcg ccataagcaa
    20461 ataagcataa atataatatt gctcaactca ccaatagata tacctaatag agctatataa
    20521 caattcatag atttattatt tatgtacata acaagtaaaa aaacaaataa tattttacca
    20581 atttgttcaa tgatttgtcc aatagcaggg atttttacat tctttatgcc atagtaatat
    20641 cctcttagta cgtttgaaag agttattata actatagcag gacaaacagc tagtataaat
    20701 agatttaatt tagcatcttt taaaaattta agtgataaaa aagaactatt gaaagatgca
    20761 actatagata taattagtgc tacgaaaaat gatatatata aagttgatat aaatagagca
    20821 ttagtattgt gtctatcgtt taaagctttt ctctttgcaa ctagacaact aagtgcagta
    20881 ggtatacctg ttgttgtaac agctaagcaa atcatcaaaa agttaaaaat catatgatag
    20941 atacctaaac ctgtttcacc tagtgctctt gataagaata ttttatataa aaaaccaaat
    21001 attcttacaa taaaattgga tataaataaa ataactgttg atagtactaa ataaggtact
    21061 ttcaaaaaaa tcacctcctt atcttttaaa tatattcaca aggaggtgta aaaatttata
    21121 attttataaa ttctggtttc aatttattaa atttagatac atatttaaat cccatatttt
    21181 ttagtaatga atatatgtag tcaaactcac atgcaacttg acttatatga tgtgaatctg
    21241 aacctgtggt tatgatttcc cctcccaagt ctttgtacat ttgaagtata tacttagatg
    21301 gggttaggtc tggaagatta tatctataac aagaggtatt tatctcgata cctttgccat
    21361 cataaatagc ttgtttaaga gtttcttcaa ttatatcaga aaatagtcta tcatctaaaa
    21421 tagtgtcata aggtgcatac cttttaatca aatctaaatg acctaagaca gagtaatctt
    21481 tataattctt aacaatatta tataaataaa gatagtaatt ttcataaact tcatattgag
    21541 ttttatcttt aaaataatta ccaaggtata agtccattgt atctatagca tggatagaac
    21601 atattataaa gtccaatgga tattgatgtg cttccttttt gtatgtatcc attaattgag
    21661 tttgaactcc aaattcaatt ccttttttta ttgatatttt atctttgtat ttgctttgaa
    21721 gagtctctaa actcttaaaa taatcttcgt agacaattga aaaagaatca tcagcatata
    21781 catcatagtc aacatggtct gtgaaacaaa tttcctccaa accgagttca atagattttt
    21841 ttaccatttc ttccattgta gattttccgt ctgtagagaa acttgagtgc atgtgatagt
    21901 cgtaaaacat aaatatttcc cctttttata aaaattttgg tattatatat acatactaaa
    21961 taatatcaaa tatatataaa agcatcaata aaatataaat acaatgagcg gtgataaatt
    22021 tggaaaaata cagaatagat ttaaatgaga aaaaatcaac taaatatata cagatattta
    22081 atcatataaa agaattgata ataaataaag agttagtaga acatgaaaaa ttgcctccaa
    22141 taagaaaact atcaaatttt ttacaagtaa ataacactac tatagttaag gtatacgagc
    22201 ttttagaaaa tgaaggatat gtatataaaa ttatagggag tggtactttt gtatcaagtt
    22261 caaattctta taaaagcggt atagataaca ggaaaaataa gattattgtg caaagagaag
    22321 atttaattca ttttgacaat gggaatccat ctaatgatat gtttcctatc gattcattta
    22381 aaaatgctgt aaatatggca ttatctaaag atggtagttc aatatttgaa tatgatgaag
    22441 gtcttggctc tgaagaatta agagaaaaaa tggtagagta tctaagtgat ggaaatataa
    22501 aaactactaa ggaaaatatt caaataatat caggtgctca acaaggtata gatatagttt
    22561 gtaaaggact tataagttat tcagatgttg tttttatgga agaaccttct tacaatggag
    22621 caattgaagt gtttaaaagt agaggtgcaa aaataatatc tatacctatg ttagatgatg
    22681 gaattgatat aggtatttta aaattaaagt tggaaaaaat aaaaccaaga cttatataca
    22741 ttatgcctaa ttttcaaaat ccaactggaa tatcttactc aacatataaa aaaaagaaat
    22801 tgatagaatt ggcagaagaa catgattttt atataataga agatgatttt ataagtgatt
    22861 ttgtttttga atcaaaggac aatagaacaa ttagaagtta tgatgataaa aatagagtag
    22921 tatatataaa aagcttttca aaaatactta tgccaggtct taggatagga attgtagaaa
    22981 tgccaaatga gttgttaaaa aaagttttat gggctaaata ttcatcagat atatcaacac
    23041 caggtttaat tcaaaaatca atgtattact atatgaataa ttttgattgg aagaattatt
    23101 tgagtcatat agagaagata tatactaata agtataaatt gtcaaaaaac ttgattaatg
    23161 acaaattgag tggtaaatta aaagttagaa aatcttgtgg aggaataaac ttttttttag
    23221 aattaccaag aggatataca tctcaagcat ttacaaattt tatgttaaac aaaggagtgt
    23281 caatgcttcc aggaacttat ttttttgaca atctagtgga tgatagattc tttaggataa
    23341 atatagctcg cccaagcatg gaagaactag aaaagggaat atctgtaata agtgataata
    23401 tagatgagtt tttttgtgaa tataaaaaca agttagatat aaaaagcaat aaattatttt
    23461 attaaataaa tagaggtttt ttaggaggaa aaaatgtttg acaagaagaa attagatagg
    23521 attaatgaat tagcaaagaa aaataaagag ggaatccttt ctgctgatga aatcaaagaa
    23581 agagaaattt taaggaaaga atatttagaa aactttagag ctcactttag atcaagacta
    23641 gatagtgtca aagtagtttc accagaagaa tatgagcaat acatgaaaaa caataaaaat
    23701 tagaacttag taggaggaaa atatgaactt aaagtacgaa tttaagaatg cttgggattt
    23761 tgaaagaaaa gaaaatacta ttgaacaaat tatgcaatat tctcgcaatt atatggagtt
    23821 tttaagtaag tcaaagactg aaagactttc agtaaaagaa attataaaat tggctaaaga
    23881 aaataactat atttcaatag atgaagctat ggaaaaagga agcataagtt gtggtgataa
    23941 gatttatgtt ataaataaag agaaggcagt agcacttttt gtaataggta aaaattatat
    24001 agaaaaaggt atgaagatta taggaagcca tatagattca ccaagattag atttaaaacc
    24061 aaatccactg tatcaagaga gtaatctagg tttttttaag actcattatt atggtggaat
    24121 aaaaaaatac cagtggacag ctcttccact tgctttacat ggtatagtta tattaaatga
    24181 tggaacaaaa gtggacatat ctattgggga agaagatagt gacccagtat tttgtgtaac
    24241 tgatttattg attcatttgg ctggagacca aatgcaaaag aaactatcag aaggaattag
    24301 tggagaagct ttaaatatat taattgggaa tattccttta gaagatgaag aaaaagaacc
    24361 tataacagca aatatattaa agattttaaa tgagaaatac aatatagttg aagaagactt
    24421 attaagtgct gaaattgaag tagttccagc tggtaaggct agagatttag ggtttgatag
    24481 gtctatggtg ttgggatatg ggcatgatga tagggtatgt tcatatgctg ctgtaaaagc
    24541 tatactagaa actgaacaac cagaatttac ttcagttgca ttatgtgtag ataaagaaga
    24601 aattggttct aaagggaata caggtatgca ctcaaagttt tttgaaaata ctgtggctga
    24661 actgattgct ttagaaggag attattgtga tattaaagta agaagagctt tagcaaatag
    24721 taaggtatta tcagctgatg tttctgctgg atatgaccca aattttggag aagcatatga
    24781 aaagagaaat tcagcatata tgggaaatgg agtagtttta actaagtata caggttcaag
    24841 aggaaagagt ggatgtaatg atgctaatgc tgaatttatg tcagaagtaa agagaatttt
    24901 taataaagga aatgtagtat ggcaaactgc tgaacttgga aaagtagacc aaggaggagg
    24961 cggaactata gcccatatat tggcaaatca aggtgcagag gttatagact gtggtgttgg
    25021 agttttaaat atgcatgcac cacatgagat tgtctctaaa gtagacatat atgaaatgta
    25081 taagggatat aaggcatttt ttaatataaa tttataagta aaaatataaa tgtaataagt
    25141 gaataaaggc tgttgtataa acaatttaat ctgttgtaca acagtcttat ttttatgtaa
    25201 aaatagcaat ttgtcacact agagaaaata aaagaaaata ctgtcaaatt aaaaattatt
    25261 taaaaaaaaa ttgacaacga tttcatcttg ttataatatg taataaagaa cttgttatat
    25321 aatgttataa aaatgttaca tagggaggtg agcaaaatgg atgaaaagag acaaaaaaaa
    25381 gaaatggatt tagaagtaaa ttcctttgtt cctttatatc aacaactata tgacaacata
    25441 aaaaaacaaa tagcatctgg catatataaa ccaggagata aacttccatc tgaaggagac
    25501 ttatgtaaag aatttaatat aagtcgtata actgtgagaa atgcacttaa tgaacttgta
    25561 aaagaagata tattatgtaa aaaacgtggt aaagggactt atgtaacaat accagagaga
    25621 atagaagcaa catgtgcagg aaatagtttt actaattctt gtcatcgtat taatgcaaaa
    25681 ccaagtacca aaataatctc tgttttaata aaaaaagcag ataagcaagt agctgaagct
    25741 ttaagtattg aactagaaga gaaggtaata tgtattaaaa gacttagatt aatagatgaa
    25801 gtacctgtta tttttgaagt tgactatttt agaatagatt acatgttttt attaaaagaa
    25861 gaattagaag ataaatcttt aatggaggtt atatctaata atataagcac attacctaaa
    25921 cgagtagaaa atatatttga agtaaaacat tcaaacaaag aatattctga ctatcttaaa
    25981 tgtgcaagta atatgccact attgaaagta agacaatcgg tgtatacaga aaataatgat
    26041 gtcttatatt acaatgagca atttataaga agtgataagt acaaatatgc agtatcagca
    26101 gaaatataat ctatatttag taggaggatg aagtatgaaa aagataatat tagcatctca
    26161 tggagatttt tcaaaagggc ttttaaatgc agtaaaaatg atagtaggag atttagcaga
    26221 ttgtgttagt tcgtatggtt tgtaccctgg acaaagtgct tcagattttg ctttagaatt
    26281 agaaaaaaca atatcagaag atatagaatg tgaatatata attttatctg acttatatgg
    26341 agcaagtgta tgtacagcaa tgttaagatt aactaatcta cataatgtta agttattttc
    26401 tggaatgaat ttgaatatgg tattagaact tcttacaagg ttttctgatg aattaacagt
    26461 tgaagatata gaacaattag tggaagaatc tagaagagga atacaaagcg tgacattaca
    26521 aattaaagaa gaagaagagg tattttagtt tatgaaaaaa aagggaatat tatcagtatt
    26581 taaaagtaaa aaaccaatta tagcaatgat acacttaaaa ggagatactc cagaagatat
    26641 ctttgaaaga gctaaaaaag agattacaat ctttgaagaa aatggtgtag atggaataat
    26701 gttagaaaat tactatggta attattatga tttagaaaga atacttgaat atgtatcaaa
    26761 agctaatttg tcaataccat atggagtaaa ttgtttaaat gttgatacta tgggatttga
    26821 actggcaaca aaatataatg ctagttatat acaggttgat tctgtagtag gtcatgttaa
    26881 acctagagac gaagctactt tagaagaatt ttttaaatta caacgttcaa aatgcccagc
    26941 gtatttaatt ggaggcgtac gttttaaata tcagccagta ttatcagaaa atgacgttga
    27001 agaagattta aaaataggaa tgacaagatg tgatgctata gcagttacag aaaatgctac
    27061 aggtcaagaa acatcaatgg aaaaaataga actgtttcgt aaaaatttag gagactttcc
    27121 attggttata gctgctggag ttacattaga aaatgcaaaa aaacagcttg aattaggaga
    27181 tatggcaata ataggaagtt actttaaaga taattacaaa gattttggga atgttagtgt
    27241 agaacacgta aaaacattca tggatgaaat caaaaaaatt agggaggaat tataatgatt
    27301 aaattagtaa gagtagacca cagacttata catggacaag tagcatttac atggacaaag
    27361 tttttaagca cagactgtat attgatagca agtgatgaat tattaaaaga tgaattaaga
    27421 atggcagggc ttaaaatggc taaaccatct aatgttaagt tagtaatgaa aagcatagca
    27481 gattctataa aagcacttaa ctcaggtgtt actgataaat ataacttatt gatactttgt
    27541 gaatctgtag aagatgttta tagacttgct aaagaggtaa aagctattaa atctataaac
    27601 cttggtggaa caaaatcaga tgataatcgt gaaaatatat ctaaagcagt gcatgtatca
    27661 aaagatgata taaagatgat taaagagtta gattcagaag gcgtaaatgt atttgtacaa
    27721 ttagttcctg atgatgatgc tacaaatgta atgaaattaa tataatttta gggggatgaa
    27781 aatggaattt acacaagtta ttttgataac tctaattgca ttctttgcat atatgcatag
    27841 ttttgtaggt tctacaatgc ataatagacc tatagttgtt gcaccacttg ttggtttagc
    27901 actaggtaat cttcatacag gtatagtaat aggttcaaca ttagaattag tatttatggg
    27961 tgcatttcct gttggggcaa gtaatcctcc tgattttgtg tctggttcta ttattgccac
    28021 agcgtttgta atattaacgg gtcaagatgt atcagcagca gttgtacttg cagttcctat
    28081 agcaactcta gtattgctta tagataactt cttaatgaca gtagtactta catggggagc
    28141 tcatattgct gaccgttatg cagaagaggg gaatattgaa ggcgtagaac gtgtgcaatt
    28201 attatttggt ataggaaata aattaatatt agcaataata gtaggtatag gattttcatt
    28261 aggtgttcca gtaatagaaa aaatactttc atttatacca tcatatgtaa ctcatggaat
    28321 ggatgttgca gcaggcgtga ttccagcaat aggttttgcc atgttagcta ggatgatgct
    28381 taataagaaa acagtagcat ttttattatt aggatttata ttggttgctt acctaaacat
    28441 aacagtaaca ggagttgcat tgtttggatt agcaatagct ttaatatatg ttaactttgt
    28501 aggagagaag gaggtaatag tagatgacaa cgagttctaa gaagttagat acaatttcac
    28561 ccgatagtaa aataacgagg aaagactttt ggaaatgctt tagaaggtca ttgacattag
    28621 attcatcttg gaactacgaa cgtatgcaaa atatagcata tgcatacatg atggcaccaa
    28681 taatccgtag attgtataaa gatgataagg aaaagaaatc taaggcttta aaaagacatt
    28741 tagaatttat gtcagttact cctcatataa gtactctttt ggtaggtata tctggagcta
    28801 tggaagaaga aaatgcaaag aataaagaat ttgatgctaa tagtataaat gctgttaaat
    28861 caagtttaat gggtccagtt tctgggatag gagattcatt tttctgggga acattaaagc
    28921 ttatagcagc tggagttggt atagctttag cttctcaggg taacataatg ggaccgattt
    28981 tattcctttt aataataaat gttcctcatt ttattatacg ctatatatgt ctagataaag
    29041 gatttaagta tggaactcaa ttctttaaag atgtgagtag ttcaagtata gtttcaaaag
    29101 ttatggaagc agcttcaatg ttagggctta tggtaattgg aggtatgact gcatctaatg
    29161 taatgttaaa acttagtgtt aatgtaggta gtggagaatg ggcagagcct attcaaactt
    29221 acttagacca aataatgcca tgtatgcttc cagctatgat attcggtatt atgtattggt
    29281 tattaggaaa aaaggtaaag acaactacga tattaatttc agttatgatt atttgtatag
    29341 tattagctgc tattggaata gtataaataa aaaaatccca agttcatttg aacgagggat
    29401 tttttgacta aattaaataa aaaagaaaga tggttcataa taatttataa gcatttatgt
    29461 gatagttata attagcttat taattagtta taaacccaaa attaagcact actttattaa
    29521 agttattata ttataatttc gaaatactca cataatctgt ttagaaattt atcttctaat
    29581 ggggaaaaag ttttcttttt agagtaaata aagaaaaatt ttctagtaaa atcaacaccc
    29641 tttattttat aacattttat tttacctgca ttgagataat caatagctga aatgtacgat
    29701 ataaaggaga ctccaagacc taatctaacc atttccttaa tagcctcatt tgattctaca
    29761 tgagctatta catttaattt acttactgga aagttatttt tactaagtgt gtttagtatt
    29821 aagtttcttg tacctgaccc ttcttttctc ataataaaat ttagataagc caattctcct
    29881 atatcgatac aattattttt attatcaatt ttaaaatctg aaggagtgat aagaacaagt
    29941 tcatcatcta ataagtctag gtactctatt tgaggattat tgattttaga accaactaat
    30001 ccaaagctta ttcgttcatt taaaatttct gatatagcat attgagaatc ataatggctt
    30061 atggaaaatt ttacatctgg ataactcata gaaaaactct tcataaaatc aggtaaaata
    30121 taagtttcag gtatggaact acatgctata tctataatac cttctatttt tcctgaatat
    30181 tcttttatat cataaattgc acgtttgcaa ttattcaata tatatatagc atgttcataa
    30241 agaatttcac cagattttgt gagtgtaata actttgttgc ttctatttat aagaacagtt
    30301 tccaactctc tctctaaatt ctgtatgtgt gcacttacag taggttgagt taaaaaaagt
    30361 tctcttgcgg cctttgaaaa actctgatgt tttgcaacag caacaaatac ttcaagttgt
    30421 ttaaaatcca ttatttaatc ctccttaaat tatctaaaac tattataaca tatttgataa
    30481 attaatagtc aaaggtgatt tttaaaatag aaaaccttta aatattcatg gagttt
//
LOCUS       DAELSY010000029        29643 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.029, whole genome shotgun sequence.
ACCESSION   DAELSY010000029 DAELSY010000000
VERSION     DAELSY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29643
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.029"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(140..2110)
                     /gene="ftsH"
                     /locus_tag="KPG09_001236"
     CDS             complement(140..2110)
                     /gene="ftsH"
                     /locus_tag="KPG09_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG0965857.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2144..3532)
                     /gene="tilS"
                     /locus_tag="KPG09_001237"
     CDS             complement(2144..3532)
                     /gene="tilS"
                     /locus_tag="KPG09_001237"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBG0965858.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(3715..4143)
                     /locus_tag="KPG09_001238"
     CDS             complement(3715..4143)
                     /locus_tag="KPG09_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBG0965859.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEENIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(4160..4966)
                     /locus_tag="KPG09_001239"
     CDS             complement(4160..4966)
                     /locus_tag="KPG09_001239"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBG0965860.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(5168..5653)
                     /locus_tag="KPG09_001240"
     CDS             complement(5168..5653)
                     /locus_tag="KPG09_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBG0965861.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(5704..6363)
                     /gene="spoIIR"
                     /locus_tag="KPG09_001241"
     CDS             complement(5704..6363)
                     /gene="spoIIR"
                     /locus_tag="KPG09_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBG0965862.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(6508..7188)
                     /locus_tag="KPG09_001242"
     CDS             complement(6508..7188)
                     /locus_tag="KPG09_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0965863.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(7172..8062)
                     /locus_tag="KPG09_001243"
     CDS             complement(7172..8062)
                     /locus_tag="KPG09_001243"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBG0965864.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(8180..9730)
                     /locus_tag="KPG09_001244"
     CDS             complement(8180..9730)
                     /locus_tag="KPG09_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBG0965865.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(9824..10090)
                     /locus_tag="KPG09_001245"
     CDS             complement(9824..10090)
                     /locus_tag="KPG09_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBG0965866.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(10238..11098)
                     /gene="yabG"
                     /locus_tag="KPG09_001246"
     CDS             complement(10238..11098)
                     /gene="yabG"
                     /locus_tag="KPG09_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBG0965867.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(11182..12120)
                     /locus_tag="KPG09_001247"
     CDS             complement(11182..12120)
                     /locus_tag="KPG09_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBG0965868.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(12230..12391)
                     /locus_tag="KPG09_001248"
     CDS             complement(12230..12391)
                     /locus_tag="KPG09_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965869.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(12401..12898)
                     /locus_tag="KPG09_001249"
     CDS             complement(12401..12898)
                     /locus_tag="KPG09_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG0965870.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(13013..13723)
                     /locus_tag="KPG09_001250"
     CDS             complement(13013..13723)
                     /locus_tag="KPG09_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBG0965871.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(13899..14429)
                     /locus_tag="KPG09_001251"
     CDS             complement(13899..14429)
                     /locus_tag="KPG09_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965872.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEIVKNVDCN
                     IVQYSNKDERRVKAKANKMAMRLIHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(14446..14676)
                     /locus_tag="KPG09_001252"
     CDS             complement(14446..14676)
                     /locus_tag="KPG09_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965873.1"
                     /translation="MSKLVSKVKKDLEIKWLGVDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(14691..16085)
                     /locus_tag="KPG09_001253"
     CDS             complement(14691..16085)
                     /locus_tag="KPG09_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG0965874.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTI
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(16211..17464)
                     /locus_tag="KPG09_001254"
     CDS             complement(16211..17464)
                     /locus_tag="KPG09_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0965875.1"
                     /translation="MENKSNFKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALSLS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGVLGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(17515..18669)
                     /locus_tag="KPG09_001255"
     CDS             complement(17515..18669)
                     /locus_tag="KPG09_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0965876.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRKLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIKKLK"
     gene            complement(19228..19797)
                     /locus_tag="KPG09_001256"
     CDS             complement(19228..19797)
                     /locus_tag="KPG09_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG0965877.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            20000..20227
                     /locus_tag="KPG09_001257"
     CDS             20000..20227
                     /locus_tag="KPG09_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG0965878.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(20446..21189)
                     /locus_tag="KPG09_001258"
     CDS             complement(20446..21189)
                     /locus_tag="KPG09_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG0965879.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYITDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            21357..22229
                     /locus_tag="KPG09_001259"
     CDS             21357..22229
                     /locus_tag="KPG09_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0965880.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            22229..22894
                     /locus_tag="KPG09_001260"
     CDS             22229..22894
                     /locus_tag="KPG09_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG0965881.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            22915..23772
                     /locus_tag="KPG09_001261"
     CDS             22915..23772
                     /locus_tag="KPG09_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0965882.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGSYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(23928..26474)
                     /locus_tag="KPG09_001262"
     CDS             complement(23928..26474)
                     /locus_tag="KPG09_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0965883.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYTSSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(26474..27160)
                     /locus_tag="KPG09_001263"
     CDS             complement(26474..27160)
                     /locus_tag="KPG09_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0965884.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(27243..28232)
                     /locus_tag="KPG09_001264"
     CDS             complement(27243..28232)
                     /locus_tag="KPG09_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0965885.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIVCEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(28219..28905)
                     /locus_tag="KPG09_001265"
     CDS             complement(28219..28905)
                     /locus_tag="KPG09_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0965886.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(29405..>29643)
                     /locus_tag="KPG09_001266"
     CDS             complement(29405..>29643)
                     /locus_tag="KPG09_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965887.1"
                     /translation="IEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRTAIEF
                     STEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT         9650 a         5370 c         3411 g        11212 t
ORIGIN      
        1 ttaaaataat aaaaaactga aagtatcaat taaattaata catgcaaata taatttgctt
       61 attaatttaa tattatactt tcagttttag tatttaatcc acatcagata ataatcttaa
      121 aatgaccaag gtcttaaaac tatttatctt tatcagattt atcttctaaa tttttattta
      181 tatcaataac attgtttctt tcttcttcat ctttttctaa ttttatagtt aattgttctt
      241 caacttcttt agaagaattt tcctctgtaa ctgcattttc aatttcattc agaggtaact
      301 ctttattaaa tgcttttaca aattgttctg catctaaagt ttcatatata agtaaagctt
      361 gtgctacata ctctaatcta tccatatttt cttgtaatat acttcttgtc ttatcatatg
      421 caccatcaac tatacgcttt gtttcttggt ctatctcaaa agcaacttct tcagagtaat
      481 ttctcttact tgaaaaacta ttacctagga atacttcatc atcactgtca aatgacattg
      541 gaccaagttt agaactcatt ccatactttg ttaccatact tcttgcagta gctgtaactc
      601 tttctaagtc atttgaagca cctgtagata catccttaag tactagctct tctgctactc
      661 taccaccaag taaaactaca atattttctt tcatttcatt ttttgtagca tagaacttat
      721 cttcaacagg aagttgcatt gtaaaccctc cagctcttcc tcttggaact attgtaactt
      781 gatgaacagg acttacttct tcaagtacat gggcacaaac tgcatgccct ccttcatggt
      841 atgctgtaag tcttctttct ttctcactta ttactttcga ttttttagcc acacctgcta
      901 taacctttgt tatagcttct tctatagttt ccatcttaat tttcttttct ctttttctag
      961 ctgttaatat tgcagcctca ttcattaagt tctctatatc agcaggagta aatcctggtg
     1021 ttcttctagc caagacatcc atttttacat catcacttaa tggtttattt cttgagtgaa
     1081 ctttaaatat agcctctcta ccttttacat ctggtgtacc aacaacaact tgtctgtcaa
     1141 atcttcctgg tctaagtaaa gctgggtcaa gtatatctgg tctatttgta gctgccatga
     1201 ttattatacc ttgatttacc ccaaatccat ccatttcaac tagaagttga ttaagagttt
     1261 gctctctttc atcatgacct ccaccaagac ctgcacctct ttttctacca actgcatcaa
     1321 tttcatctat gaatatgata gctggagcac ttttcttagc ctgttcaaat aaatctctta
     1381 ctcttgaagc accaacccca acaaacatct caacgaaatc agaaccactt atactgaaaa
     1441 agggaactcc tgcttctcct gcaactgctc ttgatagata tgttttacca gttccaggag
     1501 gtcctaccat aagcattcct tttggtattc ttgcacccaa ttcaatatat ttcttaggat
     1561 tctttaagaa atctactacc tcctgcaaat cttctttttc ctcatctagt ccagctacat
     1621 ctttaaatgt aactctagtt ttttcatcat ctttatgaac cttagctttt gatttgccaa
     1681 aactcataac ctttccgcct ccaccttgag actgattcat aaacacgaac caaataatca
     1741 ccatgaagaa tattaacagt aatgtaggca acatttcgac aaaccaaggt gtagatggct
     1801 tagcttcacc accaaaagtt agtttcccag ccttagcttg gtctaacacc tcatctgcaa
     1861 gtttatttcc catcacttct gttggaacat atgacttaaa ctttgtattt gtatctttta
     1921 tagtaccttc tacagaagtt tgatttacaa agtataatct tgatatattc tcatctgtta
     1981 attctctata tacttcagag aattttaaat cttttatttt ttctgtaggc ttacctgaga
     2041 attgaactat acctactata attataaaaa caagtagata aaaacctgcc ccttttagca
     2101 acttattcaa tatagaagcc ctcctttcat acattgcttt gccctataat ttgttaaaat
     2161 taatttttaa tacttctttt gtgttttcat caattttata attttcactg attttataat
     2221 ctccaacaca tattattcct tcactatctg tgattatagg aattttacat ctttcttctc
     2281 ttgggatttt taaatctatg aataaatctt tcactttttt acttcccata gcaagcttta
     2341 tcttatcacc ttgccttctg cttcttatta ctataccccc ttttacctta ttaaaatcaa
     2401 accctttaga tgaattgtct aatttcatac tcttatacct atctatgctc attacttggg
     2461 tctcaataat ttgttttaat tcttttattt taataaaccc attactaggt acattgtaat
     2521 aaaattcaat ttcctcatta acaatttcct cattcgttaa tattatactg tctttttttc
     2581 tataaacaaa tatatttctt ggaagagtta acattttatt tacttttgaa ttatcttcta
     2641 aagacattat atcttctata tgtctttggt caacaaaatt agtatctcct agtatatgtt
     2701 ttatggaatt tctaagaact ctaactttga tagcatcatg taagcaaaca aaatcgtcta
     2761 aatttatctc tacaaagcct ttttctttta tatttgaaac ttctctaaat ttagcttctg
     2821 cctctttctc aatataatcg ttatcacttt ttaaactatt gctcatcctc actatagatt
     2881 ctattacatt tgaattaaaa ttatccttca tatatggtaa aaggtctaac cttatcttat
     2941 ttctagtgta tatattttct aaatttgttt tatctatcct cggatttaaa ttataagctt
     3001 cacaatactc ctctatctca tttctttcca cgtctaatat gggtctaatt atgcaattat
     3061 ctctaatata atcaattccc tttaagcctt ttaagcctgt tcctctcatg atacgcatca
     3121 taacagtttc ggcttggtca ttgagattat gacctattgc tattttattg gctttaatct
     3181 tatccttaat ttcataaaac atttcatatc ttagcttcct tgctccttct tctaatgata
     3241 atccctcatt ttcacaatat tttggtacat taatagactt tacaaagaat ataattccca
     3301 tatcctcaca aagtttagat acatataatg catctttttg agcttctatc cctcttattt
     3361 gatggtttaa atgtgctgca tatatttcta tattaaaatc tttttttaat ctatttagta
     3421 catgcaataa acaaactgaa tcaggtcctc cagaaagacc taaaactatt ttatcgcctt
     3481 tttgtattaa attatgttta tttatagtac ttagtacctt atcaaatatc atatttatcc
     3541 ctcttacact tagttttatt cacttttaag tatactttct ataaaaaaac gctaaaccct
     3601 tgtatcaatt ttaagaaatt aaagtttaat ataaatataa taataccaaa taaattaaat
     3661 aaaaggtagt tttttcacta cctttataaa atttctggaa aatagccata gttattattt
     3721 agtacctatg tatatatcta ttttattcct acctaaaaat aaatcttgta cctttatatt
     3781 atagtctatt ttcaatttta tgtgttcccc ttcttgtata tttatatcta attctcttgt
     3841 atcaatatca attaataaag taccttgtgg ggtcacatac tttgtagtac tactttctcc
     3901 acatttaaac agcattgttg agttaatagc accaaacttt ttgatactaa cttgattttt
     3961 aagtatcttt atagtattag ttatttctat attttcttct ttttccttgt aaataacata
     4021 tattccatca tttttataaa aaattttacc aaaggcagtc atctctatgg tgtccatatt
     4081 tcctttttca tcatattgtc tagtatttat acttattttt acactcaacg atttttcctc
     4141 catagattct tcatataaac tagtattttt gtatttctac tttttgaatc ttatcaattt
     4201 ttttctttaa gaactctctt gctatatctg aaaatttttc aggtatatct gatacataat
     4261 attgataagt cccatgaatt tctgtattgt ttataatatt ttgcacctct aaaatctttt
     4321 ttaaatcctt tgcagtctct ttagctggat taacaagttt tatatgctct ccaacttctt
     4381 cacctattgt tctctttaaa ataggataat gagtacatcc taacactaaa gaatctatat
     4441 ttttttcttt caattcttgt aaatatatct ttgctgtcaa tttagccact tctgtatttg
     4501 cccatccttc ttcaacaata ggtacaaata aaggacatgc tttattaaca atttctatac
     4561 tctcatctat tttagatatt tccaagttgt atgcttttga acttatagtt ccttcagttc
     4621 ctattattcc tacaattttg ttttttgtag aacttacagc tgttctagct cctgcttcta
     4681 taactccaat tatcggtata tcatattttt cttgagcttc ttttaagctt cttgcagtag
     4741 cagtgttaca tgctataact atagctttta caccttttgt ttttaaaaag tttatagctt
     4801 gaaaagtata tttaattata gtttctttgg atcttgaacc atatggtatt ctggcagtat
     4861 ctccaaagta tattatatcc tcatttggta atatcttcat tatctctttt agaactgtta
     4921 atccacctaa tcccgaatca aaaactccta ttggtttatt gttcatcttt atacaacacc
     4981 tactttacat ttttgtgttt aacaacaact tatttatcgc taaaatttat atttatattc
     5041 ataattaata tattattacc taaatgatta ttatagttta ttttacacaa aaaataaagg
     5101 agctatctta aattagaaaa taatttctac aaaaagacaa ctcctttcac tacttaaaaa
     5161 tttaaattta ttttgcaaac acatgatttc ctattctcat ataaggattc aatctattta
     5221 tccatttgca agtggctatt tctggattcc aaaaatatac tgctttatta gttgggtcag
     5281 ttccataaag agcttcctga gctgctttac gtgctgagtc tgttggtgcc atctttattg
     5341 aaccatcaag cacaactgag aaagcatttt tttgatatat gacttcttct attgtgtctg
     5401 gaaatctagg gtccaatact ctatttatta ctacagctgc tacagcaacc tgtccctcat
     5461 agctttctcc tctagcttct cctgcaacca acttagataa taacatcaag tcattatcag
     5521 ataaatttat tacttctttc tttgctatat ttttttcttg tttgtttact ttcttaatat
     5581 tttctgttgg ttcatatgca aatacctctt taataggcat aaatataaaa gtcaaagcta
     5641 atagtgtagc catcagttta ctccacctgt ccatgattaa aaccactcct tacattctta
     5701 tttttaaaat tttttctcta aatccttgac tacttcaagt actttaaact taatttttac
     5761 tctacttgtt ttttttggtg tatcttgttt tattaactta tattctttat cagttaaaac
     5821 ttccttcaac ttatcatctg tagatttatc tatcactcca ttagattcat caacaaaaca
     5881 tagtggaggg aacataacac accaccagtt ttttccttca cctttcccaa tgataatctt
     5941 taatgcttta tattcaccag ctggaagaac tatattagaa tattgtttag tagggaaatt
     6001 gctatattgt atacctacct ttacactgta attatatcca tttttaagaa taatattttt
     6061 acttatttct tccagactac tatattcctt ttttataata gcttcacttt cttttatact
     6121 ttttgaattt tgaagctttg gctgtaaata ttttattact tcatctctaa cttttagttt
     6181 aagttcttga tcttcatctg tattgctgtt tgctataaca tgaaatctta ttaatttatc
     6241 tttataatct tttgatatat tgtctacttt ttttacctca ccatttatta ctattgtcat
     6301 tattgatatg actgatataa gacttaatat taatagtccc aatctaatct taatttttct
     6361 cattgaaaat tccccctcaa gataaattta tatcatcaat tatgtccaat taaatttaat
     6421 ttattcataa tattttttaa tactaataaa aaaagctatc ttaaaatgat tacaatcatt
     6481 ttaaaatagc tttttaaacc ttatatttta taagtcttta ctaatcatag ccttatctat
     6541 cataaactgc tctgcttttt ctatatgctc tgtagcatac ttcttggata gctcactatc
     6601 acctttaatt atagcatcaa gtaagtattt atgctcttca acaatattat gtgatgtatc
     6661 ataagcagac atataggtaa ctctaaatct atatacttgt tcccataatg agtttattag
     6721 ttggattaat tttttattat tagttgcttt aaatatacat tcatgaaatt ctacatcaag
     6781 ttttaataac gcatctacat cggattccaa agtgcttttt ttaaattctt ctgatatatc
     6841 tttaagcttt ttaatatctt catcactaat tctttcagcg gcaagatatg ctgctaagcc
     6901 ttctaggctt gctcttactt ctaatacatc cataatatcc tttaatgaca tatttgccac
     6961 atacgcacct tttctaggca acataacgac taaaccttct aattcaagct ttcttattgc
     7021 ctctctcact ggagtcctac ttactcctaa ttgttctgct agttgcactt ccattagcct
     7081 ttgaccaggc tttaattttc cttctaagat tgcttctctt aaattctcaa agactacatc
     7141 tcttaatggc ttgtaattat ccaagttcag tttagttaaa ttctccacaa atttcaaccc
     7201 ctttttgact gctattaaca acatacacct gtttatattt ttttaataat tctttttttc
     7261 caattagagc atcttcttta tttttaaata gtccaaaaac tgtaggacca cttccactca
     7321 tcatagaacc taaagcatta tttttcatca ttacttgttt tatatcactt atttctttgt
     7381 gtttacctat agtaacattt tcaagaatat ttaccatgct ttctgataca gcttttatat
     7441 cttcagattt taaacactct atcaaatatt tattatttgg tcttttttta atattattca
     7501 aatctaatcc ttgataaact tctttagtag aaacgaataa gtctggtttg catatcaata
     7561 tatttgtatc acatggtaaa cctttgatgt ttgttagctt ttctccaata ccttgagcta
     7621 aggcaggtct tcctgatata caaaaaggaa catctgcgcc caaattaagt cctatttctt
     7681 ttaattcatc ttcagataat ctaagttccc ataggtgatt taaacctact aatactgcgg
     7741 ctgcattaga acttcctcca gccatccctg ctgctacagg aatatttttt tctatgaata
     7801 tttctacacc tttttttata taaaatttat ttttaagtat tttggcagct ttataaacaa
     7861 tattatcttc atctaaagga atatctaaac tagtgctttt tacctttatt tcatcttcgt
     7921 ctaattcttt tatctttact atatcgtata aatctatcgt ttgcattatc atttcaacaa
     7981 aatgataacc gtcttgcctt tttcccaaga catcaatgga taaatttatt tttgctctac
     8041 tctttagtct tataaaatcc attctatcct ccttgtttaa agaataatgc atcactaaca
     8101 ataatattat actacaaaaa ataaaataat agggtattat aataccctat tatacttaat
     8161 tactttaaat tttttaatat taatctacta atacgacttt tttttctaaa ataagacact
     8221 taccaggctt aattggttca tcaagtttta tatcattaag ttctgcaatt tcattttctg
     8281 ttgtattgta tttttttgct atattccaga aagtatctcc ttctttgcat atatatacga
     8341 ttatgctagg agctttagat aaatcatata caccttggtc ttctcctttg ataataaaat
     8401 tttctgcttt cttatctaat gcttcagtaa atctcttaat tttgataatc aagtctatct
     8461 ggtccctatt caaatcaact tcgactttat caatacatgc tgtattaaac acagatgctg
     8521 tatcagttag attatccatt tctatatcat gttcaaatgg aatttcctca ctaattttat
     8581 aaaccaattt taaaccttca actgggacaa ataaaatttc aacttttatg attccttgta
     8641 taatgctttt atttccttca atataactat tttctataga catggttgga caaacgctta
     8701 caatatcttt tatttgtata tcatcattat catttcttat gccttctcta accataaatg
     8761 tttcttcact acgtcttaaa gtcttattta attgaatagg tttgtggtca aattttatta
     8821 tcttttgagg cgaatatgca tcttgtaata cttctcttgt aacttcatct gtaactttta
     8881 ccttacaaca agccatacaa tctatttcta aaagacctgt gttgctttca ctattttgct
     8941 tgaaaatatg attaaaatct gacatagaca acaatacttc ttcagtcata ccatcactaa
     9001 ctccagggac ttctacaaat tgagtaaact caatacctac tctatccaac tctacaagtt
     9061 caccctcata tgtacaagct aacggattta tttctaatac tccaccaata ataactttat
     9121 tgtcagtcac tctactttct tttactttaa cacatggatt taaacttatt atagattgaa
     9181 tttcctctgt gtttattgtt atagtatctc ttatagaact ttctgctttt tctattccaa
     9241 ctatatcttg aaaacaaatt tcctttctgt gcttttgtat gccttccact tgcgccacat
     9301 cttttacaat gtctaacctt tgcttttcaa acaaacttcc ccttatattc ataagagctc
     9361 ctactttaat ctttctctca ttcattatag tacaatccat atgctctact tctgaaaata
     9421 acatgtattc catatcttga acaacattgt ctttttctat tacttcgttt atatctactt
     9481 ttccgtctac attagaaata gtatttttat catctgcaat atagattaca ttataattaa
     9541 aactacctct gcaaagtatc ttcccatctg ccatttcaat ttttttaagt gatatgtatc
     9601 cttctgtttt tacaatttca tatacatcta attttttgtc tggtacaact gcctccgctt
     9661 caatgaatgt ttgaaatttt ccaaagtcta ttctattgtc aactttaatt acatctttaa
     9721 ttaattccat agtctacctc cttaagtatt ataaaaatat cacactatat ttatatttaa
     9781 ggagcaaaaa aaaaattaca cctattttta aataggtata attttaactt atttgtaatt
     9841 tatcttgatc tctaaaaact tgcaacttta cattagatgt taataaatct gagtaactat
     9901 aagatactct aggatatcca ttactttcat catctaactt tataacaaac acgcttggat
     9961 aaactttttc tagtataccc tcttttgtaa taatctgctt tcttccttta ttagctttca
    10021 acaatatttt ctttcctata tgtctctcta agcttactct tatcttatct agagtttgaa
    10081 cagtagccac aatatcacct tctttaacgt ttatatgttt acataatatc acatataaac
    10141 atttttgtca aatgtttaaa ctaattattt tatatatttt ttgcaaaaat gtcaatacta
    10201 tttttgaaga tatacagtat aattatgtta atttttgcta atgtaatatt gtttttggca
    10261 ttgcccatct aaattttcct cttgtttcag aaccaccaat tcccttaaca cctgtaatag
    10321 tctgctctat aacctcatca ataggaacta ctacatcaat tcttgaacaa gcaattttct
    10381 ccactataaa aacagggtct aatgcatgta tcattattct tccaggcgaa cttgcataat
    10441 ttgctcctgc acttattatt ttttcataat ttgattggca tgctcctgcg aatatgacaa
    10501 gattatccaa atttggttgc catttacgtg cttcttttac tgttttaaca aaatttgaag
    10561 agtttctata gttactcata tcttctatat ctcctctttt tattgtcatt gcatcatgac
    10621 ctgttattac tagtatatcc ggattatatt tttctagtag tgctcttact tctttatact
    10681 gattttgctc tgaaacagct acacctactg caggtattcc aagttgagca tatacatcca
    10741 agcaaatttt taaatattct ttatctccat ctatctgtag tacttttcca ggcattccat
    10801 atgtgttagg atttaattga agttttggaa ctgctctagt cattcttttt tgtctttctt
    10861 tagcttttct tactgactta tataataaat tttctacatt tttatctatt aatatatctt
    10921 ttatatcagg tgcttttact aattctaggt catcaatata agcatctgct attactctaa
    10981 atgcaattcc ctttagtata gctattttct cattattttc atctacacca aaagagacta
    11041 ttttaaatac tatatctttg ttatgtgact ttctagctac aatgtctcct accttcatag
    11101 ttaaacctcc aataaatttt tatcttagtt tatactatgt tcgatataaa actttgtttc
    11161 ctaatgtcaa aaaaacattt cttataatac ttcttttatc actcttaaag catttttata
    11221 gtatatcttt tctatatcat cttcactaaa cccttctttt tttagaggtt catacagttt
    11281 acccatctga gatatatctt caatctcaac ttttgaatct attccatcaa aatctgaccc
    11341 tagggataca acatctattc ctccaacatt tacaatatgt tttatgtgtc taaccatatc
    11401 ttcaagttta ctttcacttt tatcacttaa aaataagtgg aagaagttta tacctgtaac
    11461 tccaccttta tttgctaata ctttaatcat atcatcagtt aaatttctag aatgattcat
    11521 cattgctctt gaattagaat gtgtagcaat tattggttta gaagaaatct ttgcaatttc
    11581 ataaaatcca ccatctgaaa tatgagaaac atcaaccaac attcccaatt cattcatctt
    11641 atgtaccact tctcttccaa aatcagtaag ccctttttct ctatattcta ctttattatg
    11701 aggaaagctc aattcattca catgattcca acttattgtc atcattctaa cccctaagtc
    11761 gtaaaatttc tttaaatttt ctaatttacc ttcaagtact gcaccttctt caatagataa
    11821 aagagctgta agtttacctt ctgattggtt tttcataatt tcttcatagt tagtagctaa
    11881 agctatttca tcactatttt ttttcatttc ctcatgaaat ttatttgcca tactcatgca
    11941 ataatcaaaa ggatgtttta cttcttcagt atcaacaaaa agagcaaatg tttgagctaa
    12001 tgaatccccc tttttcaatc tgtctatatc aacagaatat ttattagact taagttcact
    12061 tgtttctaca ttttccatta attttgctat agtatcacaa tgtaaatcaa taaatttcat
    12121 atttataccc cctgtatatt ttatatttat atctaaaaat agaaatagtt gtccttatag
    12181 acaactattg acaactattt tttaatattc tttttaatat tacttaatct tattcttctt
    12241 ttaagttagg tattactttt ttaacttctt caatttgatg ccatgtttta tctatattat
    12301 tttcactaat atattttttt aaatcagtta aagatgtaaa atctttaact gataaattgt
    12361 catcatcatc tgcaaatctt aaaataaaca tgagactccc ttactttcca caacatttct
    12421 tatatttttt tccgcttcca catgggcatg aatcatttct tcctattttt gcttctttaa
    12481 ctattgtttt agacttctta taagccttta ttatttcttt tctttggtct gctggtagta
    12541 tatcttccca accttgtaaa ttatatagcc aatgagcctc aacagttacc atattgttat
    12601 ataatttttc ccaatctatt tttatagaaa cttctttatc agcttctact tcttctaaaa
    12661 ctatatcttc ttttaagctt tcacttattc catctatgaa tcccatgaaa aattcattag
    12721 tagttccaaa tttttcagcc aattcactta ctttaccttc aacaacatcc attttattat
    12781 ttaatatttc attgtatatt ccagcttcaa ctttcaaata ttcttcccag aattttactt
    12841 ctgcttcttg actttcatga ttatcactta aatttctcca ttcagtatat aaactcatac
    12901 tttttcctcc taagtatacc tatattttta tagtttttat agtataaaat cttttagtta
    12961 gattggaagt ttcttctttt tgattatacc atattctatt taaagttaac tcttatatta
    13021 tatcataatt aaacaattct tttaatactt ttatagcata ctcttggttt ataaaataag
    13081 ggcttgtcgc aattgttatt actttggctt ttttaattaa gttttttatc ttgtaaaatt
    13141 tgagttcaaa tggtatatag tccatatcac ttgaaaatat atccaaatct atatcaagaa
    13201 caaactctga ttctacttct aaatcaaatc catacagact atctattatt attaattctg
    13261 aaaatatgtt atattttaaa gcaggtttta taaaacttcc cacatttaaa acttcatttg
    13321 tatacctaaa tacatctttt agattattta catctacatc atagttctca ggctctctag
    13381 tatccttatg ttggtctaca tgcactaact tgcatccttt tgtaaattca ccctttaaca
    13441 aagattttgc ccaaaaataa aatgcatggt tatgattgtc aaacacatat atctttttat
    13501 ttcccaggac atattcgacc atattctcca atccttttgc ttttatttcc ttattttcat
    13561 ctacttcatt aaataccacc tcattgccta accttacatc atttaatgta ccatttataa
    13621 gttttggaac gtatatgctc ttattttctc ttttatcata tgaaaataca ttatttccta
    13681 ctggtttttc tatataaaat ccactatatt cataattttc cataaaactt tatttcctcc
    13741 attgttatat aaatatataa gttataattt taaaaattaa aatgcactta ttgtaaatca
    13801 tattatctat tatagaaaac atcttatata actaaatata atatcttctc tcaatatgta
    13861 ttatacaata agtgcaaaat atatttatat aaaatatact acattcctat aacaaaacca
    13921 tcagcttcaa ttggaaggta actaactctt ctattcaact ccacctttcc aaaaaatact
    13981 acccaatatg gtctaaaaat actttcaatt ttaactattt caatctctgg gattcctccc
    14041 ataaacctat gaattaatct catagccatt ttatttgcct ttgcttttac tcttcgttcg
    14101 tccttattac tatactgcac aatattacaa tccacatttt ttacaatttc atatgtagga
    14161 aaatcttgta ctatactacc agaacctgta gaaccattca ctaaaatttt taattcattt
    14221 atatcattag aatatctcct attcttgaaa atggatttcg ttttgtgaat tttaagtgtg
    14281 ataattttaa actctatata atgaagtcta acttcatgag catatttgtt ctttaatatt
    14341 agcttactaa ataaagaatt tttcccactt ttagctatct ctatagcttc ttcttttgta
    14401 attcttatag gtaacgctct tatatccata aaatcattct ctttcttatt tttttcttgt
    14461 catttttaaa actctatata tataatcaaa aaatattaca attacaactg ccataactcc
    14521 atatggaatt atttttccaa ctataatatc atttctagtc aatataccat aaaatataaa
    14581 tcctgttatt aaaaagtaca ataatactag aaaaccgtct actccaagcc attttatttc
    14641 taaatctttt tttaccttag acactaattt agacatagca gtttctcctt ttatagatta
    14701 ttttgtgtat ttaaattttc acctattcta ttaccaatca tccatgcaat aaatgaaaaa
    14761 ggtatagcta tcacaagagg ctctaactca aagctctgaa ctggaaatat tttaaaataa
    14821 ccaaaagtca ctccacctac aatcattcct aaaataccac ccatagatgt tcttcttcca
    14881 tttccaagaa gcccacctac tataggtaca aatgccgaac ccatccaaat acttcctaca
    14941 aatatacatg catcatatac attttcaaat ctaaatgcta cgctaagccc cataactgct
    15001 gctaaacata cacctattct agtaaacttt actaattgtt tctgagataa ctcttttttt
    15061 gtaattcttc catatatatc atttgctaat gtagctccag ctgctagata atagctatcc
    15121 aatgttgata ttatagaacc taaaattcct attataaata ttactcttat tccttgtggt
    15181 aatgtatcta aaactaaagt tatatatcca gcggctggag ccatctctgg ataagtagct
    15241 cttactataa gtcctgttat agttaacacc atgtcaaaac atatccatag tgaaaaacaa
    15301 gttaataagg ctcttcttcc aaccttagga gaatctgctg ctgaaaatct ttgataaaaa
    15361 gttgggtctg aataagctcc aaaagaatat acaattaaca tcaatgcttt catagctgac
    15421 ataccttttg tagggtgcat aagctgtggt gcatctttga gtgcttctgt gagaccttga
    15481 aatcctccta tttgttcaaa ttgacctggc ataaccatag ttaaaccaaa taccatacaa
    15541 aaaaacaata ccatatctgt aatggcaact cccataagcc caccaagtac tgtaagtaca
    15601 acgactactc ctaccattat taatcctatt aattctttat ctattttcca tgctacgtta
    15661 cctatagttc ccatagcagt tatttctcca agttgtgcac aatatataaa catcaaaatt
    15721 gccccaatta tagcaacatg cttaccataa aaactttcca gtaaatcagg aactgttata
    15781 ttggttctta tatgaatcct tggaccaacc cacaatgcta aaggcattct cccaatatgt
    15841 gcagctattg accaaactgc ccatgcagat attccatata tagccgcata ctctacacta
    15901 cctactgtac caactcctcc ataccatgat gcaaccaaag ttcctactat taaggaccat
    15961 ggtaaacttc gatttcctag gtaataactt tccatatctt ttacatttct agaaaaataa
    16021 taacctattc ctaataatat aaatgcaaat ataactataa tcccattttc ccaaaatgtt
    16081 aacatatcta atctccacat tcttatattt taatttttaa ctttaatatc taaatatgaa
    16141 caactctacc catttccata aaaataggta gagttatata ataattataa gtatttaagt
    16201 aatcttgtta ttaagcaatt ttattgttta aacctttatt ttctctttta tttattttat
    16261 ttaaagcata agtagcaaat atccctataa ctgcacatac tacagataaa ataaatatat
    16321 atgtatatcc tgtttgtcca tatttatcta accaattacc tgcaatagtg taataaaacg
    16381 catctggaga aaatcctata aatgaagcaa atccaactac tgctcctgta gtttctaaag
    16441 atatattaga ctctgaaaca gatgcaaagt atatacctct aaatccaaat aaaattacac
    16501 ttagtattat catgtttatt acagctatcc atataaatcc tgtactctta ggaataacca
    16561 agtaagcagc tgtacttact gtcattaata taaatcctac aaacattact cttatagatg
    16621 aaccaacttt atctgttata acaccagcaa ctggagatgc tcccattttt ataacataag
    16681 ttcttattat tgataaagca gaaactagag tcattggcat tacataaact tctgataagt
    16741 atggacttaa ataagttaaa cttgagaata ctaaataagt tgaaaatatt ataagaccaa
    16801 ttaaccaagc ctttggcatt gtaactactt ttaacaatga cttaatactt aatccttcat
    16861 ctgatgctcc actatctttt ttctcttcca ctaagaaaaa tagaagtata ccacatatta
    16921 tagatgctgc agaatataac catactacat atttgaatcc tactgttatt tcagcaaatt
    16981 taccaaagaa gtaaagacct ataaaagaaa ttaacgtacc tgatacacct gataaccctt
    17041 cataaaaacc aaatattctt ccttgttctg actcacttcc ttgcattcta acaaccttaa
    17101 cagatgctga gaagaaagtt aatattgttg taaatcccca taatacaaat attaagagta
    17161 tcatactata tgatgggaat gtagagaaat aaaaaccaag tactcctgaa gcaattaaag
    17221 aaaaagttat cagctttcta accgagaatc tatctgcaaa ccatcctcct ataaaataag
    17281 ataccattcc tactaatcca tataaactca ataagttccc tatttgtgta tggctcaatg
    17341 ataaagcttg ttgcattggg tcataaaatg atgatttcat atatggtaaa ctatacatag
    17401 ccgttgttcc aaatgccaat atgaatatta tcaagaatct tttaaaatta cttttgttct
    17461 ccattaattt tctctcctta aaattttatt gtttataatc tcattcatgt aattttattt
    17521 taattttttt attgcttgtt taaagtcatt tattatatca tttgaagatt ctaatcctac
    17581 agctattctt attagatttt ctccattcat ttgaactaat gtatgctctt cccctaagct
    17641 tgttgctatt gttgcaagct ctaaagagtt cattaatttt ctactagctt ggtctcttgt
    17701 caatccattg ataccatcct taagtttaaa tgaaactaca cctccaccta aaccattcat
    17761 ttgagtttta caaagctcat attgaggatg agattttaat gctggatact ttaccaattc
    17821 cacattctct tcttcctcta aaaactctgc tactgctaat ccattttcac aatgcttttt
    17881 catcctcata tctaacgttt tcatacctct taaaatcatc catgcactca ttggtggtaa
    17941 agatgctcct gtaaaacaac ataaacttgg ccatctaact aaatctatta attcttttga
    18001 acctgctact gctcccccta aagtatcccc atgtccattt ataaacttag taagactatg
    18061 tattactaaa tctgcaccta aatctattgg tctttgtaca attggtgtcc caaaagtact
    18121 atcaactata gctaagcttt tattttcatg agcaatttct gctattgttt ttatatctac
    18181 taagtctaat gatggattta aaggactttc tatataaact attttagtat ttggtctcat
    18241 tgcttctttt acattttgtg gatttactgc atctacaaat gtaacttcaa ctccaaattt
    18301 agataacaac tcagacatga ataatttagt gtgtgaaaat attccatctg aactaactat
    18361 atggtcacca ctttttacca atgctaatat agtagatgtt attgccccca tccctgatgt
    18421 actagatatg gcactttctg cattttcaag catagccaac ttagcttcta actctgtaag
    18481 agtaggatta cactctcttg tatacacata tccccatgtc atacacaact cttcaaaatg
    18541 ctctactgtc tttgcagcaa atgttgctgt ttgacatatt ggtgtagcca atgcacctgt
    18601 tgttgggtct gcactatgtc cccaatgtat tatttttgtc tctaaatctt gattaatatt
    18661 gttactcatt ttgatttcct cccattttga cttatttata caacgttttc cctgccaaat
    18721 ttaatcaatt tagcttagtc ttaatacatt ttttcaagta tactttttta tttgttataa
    18781 tttctaaaaa tatgacactt attttgtttt tttacagttc taattataaa ctctatagta
    18841 actatagagt caatatgttt ttgatatttt ttttacaatg tttaaaatag tcagtacaaa
    18901 aataggtata tcttagaata actaaagtca tcctaagata tacctatttt tatatcacaa
    18961 tataaataat tacatataag cattagtttt tttatcataa atagattttt caagatttat
    19021 catcttttca taaatatatt atagttaaat attttaatat tacctaacat ctacaataaa
    19081 atatactctt aaaataaaat tatataatta aaaatactct ataaaagcta aaaagttttc
    19141 tattcgctac atctaaaaat aattagatag aggtataaat gtatttagtt tatttatacc
    19201 tctagtcttt tataaattat atctctacta aattgatttt ttagcacgaa taagtgtgct
    19261 cttggagata ccagtcatct tcacaacctc tctatatggc ttagaattta ataaccccaa
    19321 tgcatattgt atctgttgat tgctgtattt ctttggccta ccatctttaa agtccttttt
    19381 ctttttagca aattcttttc ctaattgagt acgttctatt atcatatttc tctcaagctc
    19441 tgcaactgca agtaatgtag tcaaaaagaa tttacccatt gaagtatttt ctaaaagacc
    19501 tatatttaaa atatgaacac ttacttcttt agcaaataaa ccttctatta gttgtatccc
    19561 ttctgatgta cttctagcta gtctatccag cttagttgca acaagtaagt ctcccttttt
    19621 tagagatgat ataatttttt caaatttagg cctgtttgta gtagtgcctg tataagtttc
    19681 ctttattatt ttagcatcat tatatttatt caaaatttct tgctgttgct gatacaaact
    19741 gttgttgtct gtttgatttt ttgtacttac acggcaatat ccatatataa taatcatcac
    19801 ttcctaacat aaagattttg tacagaagtt acccagaagc ttgtttttac ttatctttat
    19861 ccattttact aaaacgattt ttgggtgtca atactgttaa cttttgacac cataataatt
    19921 cctttataat ttgtttgact atgtcaaaaa ttaccaatat aatgaaatta atgagtttat
    19981 acaaattgga ggaaataata tgaatagtct gttaaaagaa gcaatagatg aattaaaaaa
    20041 catccattgc ggggaagttt ttttattaaa agaattgttc aaaggctatg aatggaacag
    20101 gttggagatg aatattagat tgaaattagg aaaacttttt ttgtatgaag tggtaactaa
    20161 tcccaattta aatgtagagg tcatcaataa aaattctgct tatcagcaaa tgtataaaaa
    20221 aaaatagtga cttaaatgta tataatagta taatcttctt attgattcaa aagttaaaac
    20281 ttttaataca aaacaatcat tctattttaa catttatcaa tatgtctaaa tttatataat
    20341 taacacatac gaatagtatt gtttaaatca cattatttaa gatagataaa tcttaaccct
    20401 actccagcat aaatactaaa atagggctta gaattatcta tatttttatc ccataaaatg
    20461 cttagctctt cctcttgatt gaacatatat atctggggaa atacaataga agcgtaaatt
    20521 tataaaattt ccaaagtcag aaaacttcag ttcaaactta ggtataccaa aactttcagg
    20581 tgctatttca tcaacgccaa aatagatgat aattccatta tctgtaatat aaaatgcttg
    20641 gtcttcaggt ataactaact caacattaga ataatacatt tctctatttt catttatttg
    20701 agatttaatg taatcagtaa ttatattaat ataatcaact ccatctctaa aaatgtcatt
    20761 aagtaaaagt tcttttccag ttgtcaaatc tatattgtaa ttattcaatg tattgtattt
    20821 aggcccatag ttattagcaa accccataag gtttaatatc atactcagta cataattttt
    20881 attaaaagta actgcgtaac tggtagatac ccaatagctt tgttttggaa gtgaattttg
    20941 agaagctgcg ttattatatt ctcgttcttc ttcctgtaag ccatttttaa aattatttac
    21001 agtcattctt acagcattgt ttatgttatt taaaataact gggtttacat acgtggattg
    21061 atttctaata ttgtatataa caggatactt tacatcatat gaaaaatact tacttctttc
    21121 aaaatcttca ttaaattgta catataatat attatatgta caggtatttg tagattgata
    21181 attttccatt aaaatcctcc cccttttata aaaaaatcag atacaatata atatgcttaa
    21241 aacagtaata atgttaatag taaatgtaaa gaatagaaag tatctaaaac agcaaaaaat
    21301 gactaatttt gtggtataat acataggatt gagagttatt ttataggagg aaaaatatgt
    21361 tattattagc agataaatgg aaagattatg aacttataga tatgggaaat ggagaaaaat
    21421 tagaacgttg gggaagttat gtacttagaa gaccagaccc acaggttgta tggccaatgg
    21481 aatctgaatg ggctttatgg aaaaaccctc atggacatta tcacagaagc aataaaggtg
    21541 gaggtcaatg ggaacacaaa aaaagatatc cagaacaatg gactataaat tataaaaact
    21601 taaagtttca cattaaacca actggcttta aacatactgg attattccca gagcaagcag
    21661 ctaactggga ttggatgata gagaaaataa agaaagctaa cagacctata aaagtgttga
    21721 atctttttgc atatacaggt ggagctactg tagcatgtgc atctgctggt gcagaagtat
    21781 gtcatgttga tgctgcaaaa ggtatggtta attgggcaaa agaaaatata gaattatcag
    21841 gtcttaaaaa tcaaacagta agatttatag tggatgatgt tgtaaagttt gttgaaagag
    21901 aaataagaag aggtaaaaaa tatgatgcaa taataatgga ccctccttct tatggaagag
    21961 gtcctaaagg agaagtatgg caaatagaag aaaagttata cagttttgta gatttatgta
    22021 tgggagtttt atcagataac cctttatttt tcttaattaa ttcttataca acaggatttt
    22081 ctccaatagt tcttgaaaat gtattgtcta caacaatagg taaaaagaca aatacaggag
    22141 atatatacgg tggggaatta ggaataccag cttccagaga tggtaagata cttccttgtg
    22201 gtatatttgg aagatgggag aaaaaataat gataaaagtc atatatgaag ataaccattt
    22261 attagttgta gaaaaaccag ttaacatatt gtctcaagga gataatacta atgataaaga
    22321 tatggtaaat cttttaaaag actatattaa aaaaaagtat aacaaacctg gaaatgtata
    22381 tattggttta atacatagat tggatagacc agtaggtgga gttatggtgt ttgcaaaaac
    22441 atcaaaagca gcatctagat tgtctgagca ggtaagaagt aagactttca aaaaaactta
    22501 tctagctgtt gttcatggca agataaaatc taactcagat atactaaaag attttttata
    22561 taaaaataaa aaaactaata tggtaactgt agtgaaaaaa agccataaag atgccaaaga
    22621 agctgaactt aactataatg tattagattt taaagaaaat ctaagtttag ttcaagttaa
    22681 tttaaagact ggtcgttctc atcaaataag agttcaattt gcaagcagaa aacatccatt
    22741 atacggagac caaagatatg gcaaagaaat aaataagatt ggacaacaaa tatctctttg
    22801 gtccaataag atagaaattt gccatcctac tacaaaagaa aaaatggaat ttacatgtca
    22861 tccacctgac aaatatccat ggaatatatt ttaaatctca aatgaggtga ttatatgagt
    22921 aaaaaatttt caataggaga aatgtctaaa cttcataaaa taagcataca gacactaagg
    22981 tactatgacc aaatagattt atttaaacct gttcatgttg accaagaaag caattacaga
    23041 tattactcaa ttgaccaatt ttcacaatta gatattataa aatatttaaa atatttaggt
    23101 atgcctttaa aagaaattaa gtcaaagctt gatgaaaata atgaagaaat tctaaaatta
    23161 ctaacaaata aaattaatat agtggatgaa aaaattaaag aattagagct tataaagaag
    23221 gtactaagca caaagaaaga aactatggaa aaaggtatga aagtagatga agttggtaaa
    23281 atagtgagaa aacatattcc acgaagaagt atcttatcta tagagtatga agaaggatta
    23341 agtagtgaag aaactatgga actttccaga agacaaatag caaatatatt agaagataat
    23401 atatctgtgt tctatggtgg agtaagtgga cttgtatcta tagataaatt tttaaaagaa
    23461 gataaagtta tttatgacaa tagttttgtt actgttgaaa gagacttatt tgatataaaa
    23521 tcacaaaaac atataacaga aatttcatct ggtgatttta tttgtatgac atataaaggg
    23581 tcttacaaag aaaattatac cgcttttaag aaactggttg attatgttga atataaaaga
    23641 atacctattg aaaattatat atatgaaata cctataatag accctctgtc tacaaacaac
    23701 gaagaggaac ttctaacaga aattcaaata caagttaaaa agagagaaat tgacttaaga
    23761 tgtctagtgt agtaatctag tttacttaca catcttaata tagttgtcta ttatcaacat
    23821 atgatttttc tagatagtga ttcctaacag tgagattttc aaaaaatagg aggaaatatg
    23881 gctaatgcta attccctcct atttttaatt aatccatatc attttaatta agccatgtca
    23941 tcacgaagtg catcaataat attttctttt tttatcttat taatagtata caacattgta
    24001 ataaatacta taaataaaac actaaatata ctaattccaa tactaaacca tggaatcaca
    24061 aaatcaatat catctgtaac cattacttta tgaattagcc atgaagaaat tactgctata
    24121 ggaagtccaa agaacaatgc tctcatgcca taaaaagcac actcaaaatt catcatcttt
    24181 tggaaatcat gctcagacat tcctacagag cgaagcatgg caagttctct acgacgtaac
    24241 ttaatattag ttgaaattgt attaaacaca ttggcaatcg aaatcagaga aatcataatt
    24301 atgaaagtat aggcaaaaac attggcaata aaaatgtaat tacggttctc atccatcatt
    24361 ccatacatat tgtacagatt gtactcatta gtaactccct cactctcaat aatttgctcc
    24421 atttctgcaa ctgactgagt aggatttttc gattgaaagc ttaaactttc aattcctgta
    24481 gaaacaccag gaatctcaaa cttatctttg gctgaataag gagccaatac tctgaagctt
    24541 gctttcttca catctgaact tctgattcct ggaggagaat caggtggcac tactttgaca
    24601 aatgaaatat tcacattttg tctctcaggt atctccaact tgccattagt ttcaggagtg
    24661 agactaacat tcatagaaga actgttgaaa atatcaataa tattattatc ctttgttacc
    24721 atagaaacag caatcagttt tgcattttgt ccagtatatt cttctgctgg taatccaaga
    24781 cttttaataa tattcagata agttctatca tcaagaaatt gcatatctac tggtaattca
    24841 agcatttcac tcatagaatg tgaaccctca tagttcaaat aatcgtctgt aagctcactt
    24901 actttggcaa cacatgaata ggtcataagt ttctgataca aaccttcgta aacaccattt
    24961 gcagttttta gcttatcgta aagcttcaac atttcgttgt ctttcatacc ttgtgtagaa
    25021 aaaataatat cctgatcagt aacctctgac ctctgatttg acatctgttt taggtctgct
    25081 actaaagcac ttgtagatat aaataaaact atgcttaaaa caagcgacaa tacaatactt
    25141 cgataacgtt tcttgtttct tttaaagttt ttcatagcaa gaactccctc aagaccataa
    25201 actcgctgtg ttagttttga tgtcttcaca gatttagatt caatttttac ctcatttgtc
    25261 tgacgaatac actccatcac tggtgtgttg gcagcctttc ttgctggtat gtaagcagaa
    25321 attaagattg taaccatact taccactgct gcaacgatga ttgcagggat agacaatgtt
    25381 aatgtcaacg gaacgtttac atagagaata tttgcaaagt ttttagctat gacagaaatc
    25441 acaagtccaa tactgactat accgattgca acaccaattg gtataccaat cgcaccaatg
    25501 caaattcctt caaatagcac cgaatttcgt aactgttttg ctgtagctcc aacagatgaa
    25561 agtattccaa attgatgtgt tcgctcgttc aatgacatgt taaatgaatt atatattaga
    25621 aaaattgagc ctagcatgat tatggcaagc acaatgccac caaccgagta caaaaacata
    25681 ttgaacaaat tgttatcaga aacacctatg aagcgcaaca cattgtcatt gaaaatggaa
    25741 gcatggtctt tagtgctact tgtgtaagag cgaatctgat gtggattttt caatgtgaca
    25801 aaaaggctaa gactgtcagt cttgacttgt ttatcttctt tggttatcaa ggtatatcct
    25861 ggtgcagaag attcttcaaa tacgggcttt ctacagatac ccacaactgt ataagttttc
    25921 ttatcctgtg gcacaaaaac ttcactccca gcagtgtagg ggtcactctg accaagcttt
    25981 tcattttcac tcattcgttt ccctacatcc aaagaaatag tatcacctac tgcaaatttg
    26041 acaccaccat ctgtcgcaac tttacttgaa acaagaatct ctctactatt ttcaggtaat
    26101 cttccagaaa ttaaagttat aggcaaagta tcaaaagctt ctttgctaaa tccagcaata
    26161 aaaagatatg gtttatctgg atttctgtta ccttcaagtg ttgcatagcc aatattttca
    26221 aatgttgcag tatttgcaac ttcatcattg actctttgtt cctgtacaaa agaagaatcc
    26281 acatccaaaa actggacatg ccaatcacca tattttataa tagctccatt tgtcatatag
    26341 ttcatcaaag aaactccaaa ggtagtaact gctgtaatca tagcagctga cagaacaact
    26401 cctataattg ttacaagagt tcgagtgcga ttctttttca atccttgcag agcaactttg
    26461 ttaaaaatgt tcattaacga ttcaccttcc tctcatcacg caccactttt ccatctgaaa
    26521 tacagataat tctatctgcc tgtaaagcaa tgttttcatc atgtgtgaca ataatgagag
    26581 tttggtgata tttactatgg ctctctttca gtaactggat aatctcttgt ccatttttac
    26641 tgtctaagct acctgtaggc tcatcagcaa gcataactgc tggtgcattc atcaatgcac
    26701 gaccaatggc aacacgttgc tgttggcccc cagaaagttg atttggtaaa tgtgtttttc
    26761 gttcttttaa accaagtaat tctaacaagt catttaaccg ttcttgattg accttacgct
    26821 tatccataag tataggcaat gtgatatttt ccactacatt taatgtgggt atcagattgt
    26881 gaaactgata aatcaatcca acttgtcgcc tacgaaaaat agcaagtttt tcattgcttt
    26941 ggccatatac atcttgcccc tccagatata tctttccact tgttggtacg tctactcctg
    27001 caatggaatg tagtagtgta gatttaccag aaccagaaga accaatgatt gcagtaaact
    27061 cccctttttc aatagtaagt gagacatggt caagtgcagt aaccttattt tcattcttac
    27121 cataaacctt acataaatct tcaatcttta aaaacgccat tatataagtc tcctttcata
    27181 ttttataaat atatcataga actttcaact tacatttaag tgacatttaa atgagagatt
    27241 cgtcactttg gaaaacgaat aacaaatatt gcaccacctt gtgggtgatt ttgtgcagta
    27301 attgttcctc cttgtctcat aataatcgtc ttacaaagtg ccaatcctat tccataacca
    27361 gctgtgcttg aatttttccc acgataaaat ctgttaaata gacatggtaa gtcttctttt
    27421 ttaaagcctg ctccgttgtc atgaatagta agttgagtaa acaaaaagtt gtcctcacaa
    27481 acgatatcaa tttttccatt atcacccaca ctctccatgc agtttttaaa tatattttga
    27541 attgcttctg aaagccaatt taaatcaccc aaaatcctta ctccttttgg tatatcaata
    27601 tgtaaagtga tattgtgcag ttccatggga atcaaaagtt gatgaagtgc agaactaatc
    27661 aaattattca catcaatttg ttctttttga aagactacaa tacctgcatc taaacgtgat
    27721 aattttaaaa gggaagtaat cagccagtcc attcgtataa gtaattcctc tgtttctcgt
    27781 atcaactctt ttctctcatc ttcatcagaa gtattctcta acagtgacaa aataagagtt
    27841 actgatgtaa gaggagtgcg aagctggtgg gctatgtcag ctagtgagtc agcaaggtgt
    27901 tctttttctt ttttcagtgc gtaattttgc tctctaatac gtaacgtcat tttagttatt
    27961 tcactttgta gaatagaaag ctcaccctca tctgattcag caatatacag atggttagca
    28021 ttatgaagca caagattaat ttgttctgaa atctgtgcaa tacttttgta tcgattttta
    28081 gtaaacacaa aaaatgctat tccaaaggta gtggcagaaa caatagaaag tattccagtc
    28141 ataatactga ttgcaaatcc cagggctaca gtgagaatag taattaataa gaataaaata
    28201 gcaaattttc gaaactctct atttcgaaac ataacttccc cccaatctat accctgtccc
    28261 acgaacagtc aaaattattt gtggacttga agggtcatct tctatcttct ctcgcaagcg
    28321 tttgatgtac acagtcaatg tgttgtcatt aacaaactca cctgccgcat cccacaattc
    28381 atcaagtaat cttaccctcg taataatact tttaggattg ctaataaaca ccaataacaa
    28441 acggtattct aaagctgaaa gaaaaacctc attgccattc tttttcacaa caccggttgc
    28501 cgtatctaca taaagcccac aaatttcaaa agttgaccca aaacgcccac tttttcgtag
    28561 tgctgttcta attcgcgcaa tcagttcacg tggacgaaaa ggtttagtaa tatagtcatc
    28621 tgcacccata ttcagtccag taacaacact tgattcatca ccagaagccg tcaaaaagat
    28681 aacaggaatg tcttgctttt ctttgatttc tgtgcaaatc gtaaacccat ttccatcagg
    28741 taaagaaata tcaataagtg ccaaatcaaa tttattctta gcaatctcta caagtgcttc
    28801 acttcttgtg gatacatggg ttactataaa tccttctgaa ttaagcaaaa gaataagatt
    28861 tttggcaatt gctttatcat cctcaactaa aaatatccgt ttcatttact ttcatcctcc
    28921 tttattttat tagttttata tattccagca gagcaatagt acactactat tttacttatc
    28981 attacacaat tttcagtagt ttcttttgtt cttatataat ccatgcataa tacctccatt
    29041 gatttattat atttcttata acataaatac acaagataag acaaaaaaca taatgtaagt
    29101 gcaagttttt taactcacta acttataaac atacactcct actaacacta ctttcttgat
    29161 gacacatctt acaagcttat attcatacat caatgaaaaa ttaaatttat ccatttatta
    29221 aaataaaagg caatagcttc tatagctaat gccctttatc ttaaaatgat ttaaatacta
    29281 tacaattttt ataaatggct ataaataaac tcgtatagaa tataaaattt atctacagtc
    29341 ttttgttata caaaatttat aacctcttat agaacaaatt taccactttg taaacttatc
    29401 acttttactc agctatatta taaactttta atttatcttt gtttaaatgt ttcttcttaa
    29461 cttctactaa agcttttagc gtatccaaag atgtcattat ttcagtagaa aattcaatgg
    29521 ctgttcttct catcttaaat ccatctcttg tagagtcatt tcctttagtt ggtgtattta
    29581 ttactatatc aacttgttta tttcttatag catctaatat gtttggtcta ggctcttcaa
    29641 ttc
//
LOCUS       DAELSY010000030        29396 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.030, whole genome shotgun sequence.
ACCESSION   DAELSY010000030 DAELSY010000000
VERSION     DAELSY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29396
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.030"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..970)
                     /locus_tag="KPG09_001267"
     CDS             complement(<1..970)
                     /locus_tag="KPG09_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0965888.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNED
                     TINKILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVS
                     REASILRLNGYSKLDIIARVIKNIFRIMILSSVTALLIYIIYILKLNIGVRACLYFAM
                     FSIICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLK"
     gene            complement(1385..2017)
                     /locus_tag="KPG09_001268"
     CDS             complement(1385..2017)
                     /locus_tag="KPG09_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBG0965889.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINLENLHIWLENGVDVCGFGGLLTKGTIEDITENAKKIREIIKNIREI"
     gene            complement(2123..3028)
                     /gene="dapA"
                     /locus_tag="KPG09_001269"
     CDS             complement(2123..3028)
                     /gene="dapA"
                     /locus_tag="KPG09_001269"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG0965890.1"
                     /translation="MKYKGIICAMITPFDEKQNINSQATCKLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVIDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEVTQNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            complement(3033..3632)
                     /locus_tag="KPG09_001270"
     CDS             complement(3033..3632)
                     /locus_tag="KPG09_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0965891.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIVLQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            complement(3690..4961)
                     /locus_tag="KPG09_001271"
     CDS             complement(3690..4961)
                     /locus_tag="KPG09_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBG0965892.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            complement(4988..5275)
                     /locus_tag="KPG09_001272"
     CDS             complement(4988..5275)
                     /locus_tag="KPG09_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0965893.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            complement(5290..7377)
                     /locus_tag="KPG09_001273"
     CDS             complement(5290..7377)
                     /locus_tag="KPG09_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0965894.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPYNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIYLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            complement(7328..7483)
                     /locus_tag="KPG09_001274"
     CDS             complement(7328..7483)
                     /locus_tag="KPG09_001274"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965895.1"
                     /translation="MKLKTIKFSNKISIVEVMKESNDNMKTNLNKKVGVNGSGQRNCI
                     NSPDFIK"
     gene            complement(7944..8798)
                     /locus_tag="KPG09_001275"
     CDS             complement(7944..8798)
                     /locus_tag="KPG09_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBG0965896.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            complement(8830..9342)
                     /locus_tag="KPG09_001276"
     CDS             complement(8830..9342)
                     /locus_tag="KPG09_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBG0965897.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDKKKGN"
     gene            complement(9335..9559)
                     /locus_tag="KPG09_001277"
     CDS             complement(9335..9559)
                     /locus_tag="KPG09_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965898.1"
                     /translation="MNEKPSFKQIEIILAVIFLVLTYYKIGLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            complement(9619..10572)
                     /locus_tag="KPG09_001278"
     CDS             complement(9619..10572)
                     /locus_tag="KPG09_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG0965899.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLESGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            complement(10600..11988)
                     /locus_tag="KPG09_001279"
     CDS             complement(10600..11988)
                     /locus_tag="KPG09_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965900.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPNNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKAGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            12261..13142
                     /locus_tag="KPG09_001280"
     CDS             12261..13142
                     /locus_tag="KPG09_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0965901.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            complement(13329..14321)
                     /locus_tag="KPG09_001281"
     CDS             complement(13329..14321)
                     /locus_tag="KPG09_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBG0965902.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKKIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(14326..15066)
                     /locus_tag="KPG09_001282"
     CDS             complement(14326..15066)
                     /locus_tag="KPG09_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBG0965903.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLVGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGDTKVEDIGVLYKIMKELLG"
     gene            complement(15131..16240)
                     /locus_tag="KPG09_001283"
     CDS             complement(15131..16240)
                     /locus_tag="KPG09_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBG0965904.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFL
                     IIEELVNKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEINFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDNIYNRIKNIVES"
     gene            complement(16258..17391)
                     /locus_tag="KPG09_001284"
     CDS             complement(16258..17391)
                     /locus_tag="KPG09_001284"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBG0965905.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNHIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGEFY
                     EDTKKYKTNVYALINIAKQGITSQDELSNMMNIDEYELKNAVRKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEAKERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGNSIEENE"
     gene            complement(17393..18487)
                     /locus_tag="KPG09_001285"
     CDS             complement(17393..18487)
                     /locus_tag="KPG09_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG0965906.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMVSKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNEYQVVFNVVAKEKTSEVEKIIMVNEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNKNIFKRGDVVIGNDDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            complement(18624..19925)
                     /locus_tag="KPG09_001286"
     CDS             complement(18624..19925)
                     /locus_tag="KPG09_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0965907.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKATLDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            complement(20149..20850)
                     /locus_tag="KPG09_001287"
     CDS             complement(20149..20850)
                     /locus_tag="KPG09_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0965908.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            complement(21063..21380)
                     /locus_tag="KPG09_001288"
     CDS             complement(21063..21380)
                     /locus_tag="KPG09_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0965909.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SKEVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            complement(21428..21739)
                     /locus_tag="KPG09_001289"
     CDS             complement(21428..21739)
                     /locus_tag="KPG09_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0965910.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            complement(21888..22367)
                     /gene="rlmH"
                     /locus_tag="KPG09_001290"
     CDS             complement(21888..22367)
                     /gene="rlmH"
                     /locus_tag="KPG09_001290"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBG0965911.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIVLAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            complement(22435..24537)
                     /locus_tag="KPG09_001291"
     CDS             complement(22435..24537)
                     /locus_tag="KPG09_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBG0965912.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEKEI"
     gene            complement(24550..25347)
                     /locus_tag="KPG09_001292"
     CDS             complement(24550..25347)
                     /locus_tag="KPG09_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0965913.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            complement(25449..26912)
                     /locus_tag="KPG09_001293"
     CDS             complement(25449..26912)
                     /locus_tag="KPG09_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBG0965914.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            27037..28095
                     /locus_tag="KPG09_001294"
     CDS             27037..28095
                     /locus_tag="KPG09_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG0965915.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            28104..29096
                     /locus_tag="KPG09_001295"
     CDS             28104..29096
                     /locus_tag="KPG09_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0965916.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKEKLLSESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
BASE COUNT         9741 a         5218 c         3296 g        11141 t
ORIGIN      
        1 ttttcaatgt gaaatgcatt atatttacaa aaccataaga tttttttcca tttagagata
       61 gcacatattt agaacttcta ctattaaaac caactattaa tatagatatc aaaatattta
      121 tcaaaataca tataatcgaa aacatagcaa agtataagca tgctctaaca cctatattta
      181 attttaaaat atagattata tatattaata atgctgtgac agatgataaa atcataattc
      241 taaatatatt ttttataacc ctagcaatta tatctagctt agagtatcca tttaatctta
      301 aaatagaagc ctctctactc acactaattg aataatgtat tattgtagcc aagaaacaaa
      361 ggataattag tattaaatct ctaattattg aaggatttaa gtataaattt gagttaacat
      421 atctatcatg taatcttgct acaaaaatac ttctgtctac atttagttct cttaatattt
      481 tatttattgt atcttcattt tgagttgaaa tatataagat tcctgattct ccaacttcac
      541 ttattttacc aaaatcccta attaaaattg atatgtcttt actcatagtt gagaatttac
      601 ctacttgatt actattatta ctattattag tgtttatatt actaataaat tcatttgaat
      661 tagtattagg aaattttcct gattttaact ttatattgtt attcaaactt gtatcagtcg
      721 tatatataga taatttttca ttattattaa aaacatactt tgatatagcc acattatttt
      781 catctgcaat atctttaatc cacttactat aatttttatt taacttttta taatctttaa
      841 acattacaga tatacttgtt gtatcgttat ataaaatatt attaatttca ttatctttaa
      901 ctatatttag acctaataaa gatactatta atagttcaat tgtaaataat acatatatta
      961 tctttttcaa ttatagctcc ctcctaattg tttttattct aaaagcaaaa tatattttac
     1021 tttagttaaa gtataatgct ttgtaccttt tctaaacaac tactatattg tcacattcta
     1081 aatggcaagt gtctatttcg tcacaaagat aaaaataacc ttaaaacagt taatttttta
     1141 taatcaactg ttttaaggtt attttttatt tttagcaaac aatattttaa tagtggtaca
     1201 aaattagtat aattcattaa tatagattct agtattttta gcaatcaact aaaataatat
     1261 cttatttaaa acaaaattta atttaagttc aatataaact ttacctcaac tagaaaatat
     1321 ttcttctaac ttaggaatta atgtgtggat atctcgtcaa tagaaaacta attcatcctc
     1381 catgttaaat ttctcttata tttttaatga tttcacgaat ctttttggca ttttcagtaa
     1441 tatcctcaat agttccttta gttaataaac caccaaatcc acatacatca actccatttt
     1501 ctaaccatat atgtaggttt tcaagattaa ttcctccact tgctaaaact ggcatatcag
     1561 gtattggtgt cttaaatacc tttactagtt taggtccata gaaatctgat attgggaaag
     1621 cttttataaa agtcgctcct aaacttagtg catctactgc ctcagtcaat gatgtacatc
     1681 ctggagcata aggaatctga tatctattgc aaatcttcgc tacaccttca ttgtaatttg
     1741 gtgcaataat aaattgagct ccatgtaata tagcatgacg agctgtttca ctatccaaaa
     1801 ctgttcctgc tcccacacaa agtatttcac catacttttt attaagccct tgaattattt
     1861 ctccagcatt atttagggta taactcattt ctataactgt tataccacct tttatacatc
     1921 cttcagcaat ttcacatgca cggttaagat tttcttctct tacaattgca agagccccac
     1981 attctgacat tcttttggtt atattcatct ttttcatatt atcacctcta tactcatcaa
     2041 accactttac ttattcatta attttagtca tgttttataa tatatatatt aattatatac
     2101 cagtgtaatt tccctattta tattatctat tctcactact tttattaagt attgtcctat
     2161 aattttcaac tactcgttta atttcttcaa gtacttcccc agttggttca gttacaggaa
     2221 gtcgtgcagt tccaacatta attccattca agacaatagt tttcttcata acagatggaa
     2281 ttgttccaag ttttagtata cgtcgaagtt cttctatact actttgagct ttttgagctt
     2341 cttccaaatc ccctttcata aagttttcat agatagaaat accaatctct gtgaggaagt
     2401 ttgttgttgc tgcaacagct ccttgactac cagcttttag actatccaga ataagtgaat
     2461 ctgagcctga aaacacacta aaattctcat tctgcgttac ttcaatataa gctttcatgt
     2521 tgtcaagttt accactacta tccttaattc caacaatatt ctttatcttt gataattcac
     2581 gtactgactc tggttcaata ttaattccag tcttacctgg catattatac attataatag
     2641 gtaaattaac tgaattagca atagttttgt aatgtaagat taattcctgt tgtgttggtg
     2701 taacaaagta tggtgtaata atagataatg cacttgctcc aatcgcttcc attctcttgg
     2761 ataaatctat aacttcacga gttgaattac cacctgttcc aacaaaaaca ggtacacgat
     2821 tatttgtatg attaataaca atctttgcaa attcaacttt ttcatcatct gttaacacat
     2881 gacattcacc attagtaccc aaaataaata atccataaat ccccttttca atcaaataat
     2941 caattaattt acatgttgct tgtgaattaa tattttgctt ttcatcaaat ggtgttatca
     3001 ttgcacatat aatcccttta tatttcataa aattatattc cctctctaat agactttcca
     3061 agacttcctg ctggatgcca tttcaaatac tgctgtgtat taagaccttt agcatattgt
     3121 aatacaacac ttaatgattg taatactatt gtttcagcta aaattgaagc acgaggagct
     3181 ttattaagac aatctccttc ctgtttaacg cctgcgaaca ggaatatatc agaaatattt
     3241 tttaaaaatg aactctcatt accacttaca ccaattattt ttgctccatt tacttttaga
     3301 gtcttaagag ttgcttttaa ctcttgagtt tcaccactat ttgatattgc aataacaaca
     3361 tctccttcaa ctacttgacc acttgaccca tgtactgctt ctgtaccatg caatatatat
     3421 gctgatgttc cagtagatga aagcaatgat gaaatatatc ctgatacatg tcctggttta
     3481 ccaataccag tcacatgtac acgtccgtga tttttttcag cttcaagaat taattcgcat
     3541 gctttattta tagatgtctc atctattgag gcaataaatt ctgacaattc attagatata
     3601 ttatctaaaa attcagataa tgtattttcc ataagatgta tcctccttga aatcatagta
     3661 actatgacta tacttttata taatttagtt taaataggtt ttataaattg taaaatatta
     3721 tgtaatatcg aaccaactac gttgtaatca atattaggga aagttgctcc ttctatacca
     3781 agagtcccat aaactggata tagtataact ggtaaaaatg caagtaataa cccaacaaca
     3841 aatgacccag caatagcccc tctccatcct cctgaagtat tgccaaatat tgcagctgta
     3901 cctcctgaga agaaacatat gtgagcagct ggaataatta ctacaggaaa tatactagga
     3961 aatgatgcca taatagcaat tgctaataga ccagcagcat atgctgatat aaacccaata
     4021 ataactgcat ttggtgcata tggaaatact gttggaacat ctagcgcagg acgtgaacct
     4081 ggaatatact tttctgaaat gattacaaat gctgcagtta tttcagctaa gaacatacga
     4141 acacctgaca tcaatattga catacctgcc acaaatgtaa atgcctctat aagcggaaaa
     4201 accaaccaat gtgttgaacc agcaatttct tgagtaaatg cttgaccagc ttttaatgtt
     4261 gcaatataga ataagattaa cataactatg gatagaccca ttaagaagtc tctgaatatt
     4321 gataaccact taggaaattc tatagtctct gttgtatcat ctttatgttt tgaaaataaa
     4381 cgcccaatat aacctgataa accataacca atcatgttaa agtgaccaat agcttgttcg
     4441 tcgccacctg ttaattcacg cataaatggt tgacagaatt gaggtagtgc agcagacata
     4501 aaccctaaga tagttccacc tattaaaata gtgattgtat tatcaaatcc atgtgctttc
     4561 atgattaaaa ctaatacaca agaaaagaat aaactatgac ctgttgtaaa gaaaatattc
     4621 ttaaactttg taatacgtgc aaatactaaa tttaatatga atccaagaat taatgtacat
     4681 gaaactgcaa aaccatatgt accttgtgca agtgctgtag ctgcttctgg tgaagctata
     4741 acaccagtaa ttccaaagcc atgttggaac agtttattga aattttgaag tgctcctgtc
     4801 atagcagagg caccaatacc aaatatcaaa aaaccgataa gtgtttttga agaccctgta
     4861 aatacttcaa cggctgattt cttttgaaga atcaatccta tacatgcaac tacgccaagc
     4921 aaaacagcag gtgtactcag caaactaata ataaactcca taaatattcc tccttatcat
     4981 attaaattta tccctctttt gaagcaagga atttttctag tgctgtcttt atttcattac
     5041 ggtcaacaat attgtgaatc ccaattatat caaacttcaa atttttattt tttaattctt
     5101 cagcaacatc aattaatgtt tctacagcat ctccattgaa tccatttagt gaatccaatg
     5161 aaccatgttc tacttcaatt ggaaagttat tttccttaat aatttgttga gtctttattt
     5221 ttaacattaa acttgagcca actcctgctc tacatgcgat taaaatttta tacataatta
     5281 aatcctcctt tataattcgt gtgtttttat aaaatcaata atttctttag cctctttagc
     5341 actatctaaa accttaaaaa actcatcttc ttccaatagt tcaactagct cagacatagc
     5401 acgtaaatgg ctattattgt ctattgcact tagacaaaat acatatttta cagggtcgtt
     5461 ttcgttattc ccaaactcaa taggactatc taaagtagca atgcctattg caatctcttt
     5521 tgacccagct tctggtctag catgtggaag tgcaacatgt tttgtaatta ctatgtatgg
     5581 tccagatgct tttgctgagt taatcatagc atctacatac ccttcagtta ctttgttatt
     5641 ttcaactaat acagaagctg actgtctaat agcttcctcc caattcttag cttttacctt
     5701 tatttttact agtgtttcat tagtaatctc acttagcata ggcccctctc cttccttaga
     5761 tgtaaaattc tctatctgtg taaaataagc taatagctca cttgaaagaa tactttcaga
     5821 ggttacattt gcatattttt ttattatatc cataacctca tctattttag gttgtcttaa
     5881 ataaatatca cctaattgaa tatacacttc acgtgtaatc ttatattttt cttttgtggt
     5941 cataacaggg cttactttaa ttactggtac atctgttttt aaaccctctg cgttataatt
     6001 agtagtaaat acgatatcta caggttcact tatattttct aatccaactg attccgacat
     6061 tggtataaag tgtaagtcag gaaataagtt tgtaagttct gcatgtaaaa tagctgaact
     6121 accaacgcca tttgaacaaa ctattaatgc tattcttttt ctaaaaccat caaattcttt
     6181 tttatttgaa aaaagtgttg caaagtgcat tgtaagatat gctaactctt cttcctgaat
     6241 ttcttctcca aataatccgc aaaactcttt cattgcatta cttacaattc tataaactaa
     6301 tttatattct tcttttactt tttcacaaat cggattatag ataggtaatt taaataaaag
     6361 tctatagtag gcaggtctaa aatgagaata taattgttta aatatttttt cttggcttat
     6421 atagtaaact ccgcttaaat atccaaactt tgtcatcatt ttatttacaa tttttgaaat
     6481 tacatcctta tcttctgtat cttcttcata atttcctacc gatataccta aaatccaagc
     6541 actaatatac attatattat aaggctttat cttatcctca ctcccatata cttctaataa
     6601 ttccttagca aatttatact ccttcattga actcataact gaaatattag gaatatctac
     6661 attttcctga gtaactattc tagttgaata catccttgct tttagaaaga taaatatata
     6721 tataaattct attaatctat cttcaacaaa agtaatttca tattttttta ttagctttaa
     6781 tataattttt ttagcctctt caaaactatc taaattatat tctttaataa aaatatcaaa
     6841 tactcttgaa cttttctcat tagacaaagt ttctataatg tttttaataa gcactctcct
     6901 aatttccatc tcattgccaa ctatgtaata tccatttata cgattgttct taactttaat
     6961 gttcttcttt tcaagctctg gtatcaaatc cttgaaatca agatttacag tagacctact
     7021 tacttttata gaatttataa aatgattaat tgaaatatcc tccatgttaa taaaaagcat
     7081 taaatacata tataaaagtc tttctgtctt actaaaataa taagtattct tagaatagtt
     7141 tttttctaaa atttctttaa ttgctttttt tgtttctttt ctaacgataa tatctttatc
     7201 agctcttaaa catataagtg gtacattttt tactttaagc atgtcattga ttttatttat
     7261 tctataagta atttgtctct ttgatgaatt agactcctct tgtaatccat tagttgtaat
     7321 caatgtgcta tttaataaaa tctggagaat tgatacaatt tctttgtcca ctaccattaa
     7381 cccctacttt cttattaaga ttagtcttca tattatcatt ggattctttc atcacctcca
     7441 caatacttat tttatttgag aattttatcg ttttcaattt catttaagta taacttttgt
     7501 tttagattga gacaaaaatt aaacttatct aaaatagcta ctatattaca cttatagatt
     7561 ttataaataa aaaagggttc cttatgtaaa gaaaccaaaa aaaatttatt taaagaatca
     7621 aagcgatact aattatgcat aaattaccat aaataaaagg cacattaatt taaaataaaa
     7681 ttaatgtgcc ttatttataa ttctaatata ttaaatttat tttttaactt gtagtttaat
     7741 atcctttttc ttcaattaat tcatataaat aaagtattta ataataaatg ttccttatga
     7801 ctttaatatt ttaaaccaag aaagttaaag tttctctcat ataataaatt gacacttatc
     7861 tatttcattc ttttaagtgt acaataagtc ttcttctagc agtaagagta agtacttcct
     7921 ctacttatca caaactataa tttttatctc attggtatat atccacgata ctgtgagtat
     7981 gcatccatag ttgctatatc acctaagaat actgatgcga tattgaattg tggttttaaa
     8041 tatgggaact cctcttcttg tgcccattct ggttttttaa taaacgactt tgtctcttca
     8101 accacagctt tataccattc ctcttcccct tcaaaccata gttctgatac acggtcaaaa
     8161 gcagttgctc cataattaat cattatttta ctactgacaa aacgattaac atagcaacag
     8221 tttgtaaagt atggcactac ctcatcatag aaccattttt ctccttcttc tttagagata
     8281 ccatctggat acttaatcat aaattgccaa cgatagtttg gtccatcttg tacagtacgt
     8341 ccttttcctc taaagtcttc ttcccagttt attggaacaa aagcaaatat aaatggtggg
     8401 catccattat ctccacctac tgcacgtcct gcatctccac tttctgcatt ttcatgaacc
     8461 cttttatttt ctacatctgg tatacaccct tgccaacgta gaacatccat aggcatatat
     8521 tctgtcataa ttctatttgc catctcaggc atatgttcat ctacaagcca ataatgttct
     8581 gttagttgca tcttacgtgc accaaaacgc tcaccttcat ttggtgtagg atatgcttga
     8641 taaaaggcat atttagtaca atatggtcca aaagtgctaa tactatctgg aatatggtga
     8701 cgatataacc agtccattaa cttaggtcta tattcttcct tggcaagtcc tacatatatt
     8761 aaacttctca ttattttaaa ttcaaaaccc atagccattc tcctataatc aaagtatatt
     8821 cacatgcttt caatttccct ttttcttatc tttactcaaa atatatattg taaacaaatc
     8881 tacacctgca cccacgtacc atactcctac tgccatagct acagcaattt gaatacaaat
     8941 atcttgtaaa ttaataatat tttcacctgt cataatagtt agacctatag cccaaccaca
     9001 aagccatgat ggtaggaaaa ttattttttc aaataaggat gcaattacaa ctgcaaaaaa
     9061 acctaatata ctaagtgtta aaaataattc taggttttga ttgaaattca tatatcccat
     9121 aattaaaatg gctaacactg cccataaatc tccacaccaa aaaccaaaca ttatttttaa
     9181 tccatctttt tgtttattac cattagttac atataatcct gcacagatta gtgctacagc
     9241 tccagttttt attcctatat aaggtgctaa tactgcccat aaaggaggta gtaaagcaat
     9301 ccccatagac agtgttaata tttctttttt ctttctatcc atttcctata ttgtaattag
     9361 tagcttccct agcttgcctt atctccaata gcttaattgc tatttgattt acaatcatag
     9421 atactgcaaa ataacttaag tataatggta taaatataac aataaatgct ggagttaatc
     9481 caatcttgta atatgtaagt accaaaaaaa taactgctaa aataatttca atttgtttaa
     9541 aacttggctt ttcattcata caataagcct cctataacat ctaacatccc ttaaaaaaga
     9601 gtcttagtat tttaattatt atcctaatga aactccagct tgcttttctt tagttaatgg
     9661 ctctataaaa ttataagatg tttttctttc tttaaattct tgtgttttac gaacttctgc
     9721 ataatgacct tccattttct taatatcatc accatgtata aacttacctg gtaaagtatc
     9781 atgagcatga cctaattgct cttctggaaa actaaatata gtttttccac ttatagtatc
     9841 taactctcca actataccac atagagcaca aattgccttt ttagtacctg actcaaggta
     9901 gaataatcta ttgtggcaat gtggacatac accttcttct ccttgatatg aagctttttc
     9961 aaaatctttt gctgcttcaa ctatatttaa accaatttga tgaacttgaa caagccttgt
    10021 atcatccatt actaattcct ttgcccatgg gaaccaagca ttatctataa ctttccacat
    10081 tggtatgaga gctagcatag catgttcaca ttgaactctt gtaccccagt cagaacctcc
    10141 tattcccata aatgatatta ctttatcatt aagcattctt gagtctggaa tttttcctcc
    10201 tatagcttct gcaacttttg ttcctataat gttatttcct ctatctgctc tgggtccaaa
    10261 acggtccatt atagtatgga ataatcctga tgccccttct tcaaaaatag ggtctgaaac
    10321 tactatacca tctgcatctt tcattttgtc aagcaaccat tcaaaatcat cttttaatac
    10381 acacatacta cctttcccgg ataataaagt tttaacgcac gcacaacatc cagtacaatg
    10441 tttaatgtta atagatgaca tttgaataaa ttctacttca gcccctgctt ctttggcacc
    10501 tttaagtgca actttacaca ttgtgtcatt atttccattt tttgttccaa aagaaatacc
    10561 tagtattttc attttcaact ctccctcttt agtatttatt taatcttctt tcatttttaa
    10621 tatatagaat ccatcatcta aagcatctat gatgatgtag cctctatcat caacgataag
    10681 atcttctgtt actgccaagc gtggtcctgg gaatcctggg aaataagatt cttctggtgt
    10741 tttatttgga tttggtggta taaaatatgc tagctctttg atataatatg ggtctgatac
    10801 atcataaacc cttagccctg catggaaata acagcaatat actctatctc ctctttgctc
    10861 taaccatggc ttattatcca ttggttcatg aagattatgt ggaccaaatg gccctgcttt
    10921 tcctaatcct gcaacattaa agttaggata agggaaatct tttggaactt caggatatgg
    10981 gaattgagca attaatgttg gttttgttgg gtcactaaca tcaaccatat gtatattatt
    11041 catagcttga acatctgtaa tgttatctgg tttaaagaac tggaatcttt ctccctcatt
    11101 ttgaacaacg actaaatctc ttcctggcaa tggtaatgct gtatgagttc ttgcacctgc
    11161 aagtctacta gaaaatgcag gcataaatgg caattcacct aagcatcttg gtcttgttaa
    11221 atcttcaaca tctaatacaa ctaaaccagc tcctccataa ccacaatatg cttttccgtc
    11281 tcttacaaat ggaggtccat gaagccatcc tttatccata aattctgggc aatgaggtgc
    11341 tcctgcatca aaagttctat ttggatatcc atctgcatat tggtctggtc tccaccattt
    11401 acctatttcc actggattat taggatttat tatatctatg accctataaa tcagaccttc
    11461 aaagccaaca caatcacttg ataaatgtac atatcttcca ccattgtaca tgaatctatg
    11521 aacacccatt acatgcttta agccacaatc ccaatatcct aaaaacttag gatttaaagg
    11581 gtcttctttt aaactgtata ttctaattcc tgcttcacac ttaatatttg ctaatttagc
    11641 ttggtcaaca agtgctcctg gtccacttcc acaactcatt gctactatca ttaaatcatc
    11701 tgctatttga atttttggag ttgttgttgt aggatactct ttagggtcta acatttgaaa
    11761 atgttttata aattgtggat tataagggtc tgtaatatcg ctaatcatta ctccattttt
    11821 tttatttcct ccaaaacagc atccatataa atagtatttt tcatctcttt tgtacagagc
    11881 catttgaaac attccacaag ttccatctaa gttattaaag gaacaaactt caagattttt
    11941 aatatatccg ttatttccgt tgccttttgc ataaaattta tcttccattt tttctccctt
    12001 cttatttaaa gtattatcaa ttgatatact ttcattttat gagacaaact tacttaaatc
    12061 caatatcgtt ttcattggaa tatataagtt attcttatta ataaatttat aaaatctaat
    12121 gtaaagatat acttaaaaca tatattttta tacaaatatt ttctaatatt ctttaaaagt
    12181 aaattttctt atactataat aagctttaat tatagataaa taaggtttac aaacaatcaa
    12241 gtttaaatag gggtgattat ttgaatacta aacaattaga atattttata tctgtagcag
    12301 aaaatctaag ttttaccaaa acagcagaaa aattttatat ttctcaaact gctgttacac
    12361 agcaaataaa agctttagaa gaacaaataa atgtaactct atttacaaga agtaaacgtc
    12421 atgttgaact aacaccagcc ggaaaagttt ttttatctga ggcacgtact ataataaaaa
    12481 atatcaatga tgctattgca aaaacacaac aatttgcaca tggttttttt ggaactttga
    12541 gcattggaac cttaatagga tatgaaaaaa acaaacttca acaatactta aaagaatttt
    12601 ctaatacata ccctaatata tctatggata tttgtactaa tgaaataacc gaacttttaa
    12661 acttagtaaa aaataattct atggacctcg cttttgttat taatccagaa aatcaaccat
    12721 taaaagattt tgaatataaa actgtagaaa gatattcttt ggtagctctt ttacctcctg
    12781 gtcatccact ttacaatgag gattctatcg atttaattga attacaacat gataaattta
    12841 tttttgtaaa agaaacaggg gatgagtatg gacaaaagtc aatgatacaa aatagatatc
    12901 gtgaagcggg gtttgtacct aatgtggtac aacgttcaaa taatcttaat actattgaat
    12961 cattggtatc ttctaacatg ggaatatcca ttttaccttc attttgtgtt acagatacaa
    13021 tgatagaacg tgatattgca atagtaccta taaaagaagt agaaaataga attgaagttg
    13081 tggttgtatg gaataaaaaa aatacaaatc cagcattaga aaagttcatt tctataatat
    13141 aaaattaatc aatattaact ataaaaatag cattaaccat ttttatatgg ttaatgctat
    13201 tttctactat tttattatct aacttttaat ttaatattta taatcgttaa aaataactct
    13261 gtttaacaaa tataagcaag tcaaaagtgt gtctaaaaaa gataaaaatt ttattatata
    13321 caaatgtatt atctcaatat atctattttt tcattgttca tcagtctaag tacatgacct
    13381 gtgcttacta aagaattatc cccataagtt gtatgagcta acaccgaact agcaacccca
    13441 aactcaacta tatcttcact cttcatattt tccaaatatg aatatataat tcctgccaca
    13501 tatccatccc cagcaccaac tctgtcatat atttctaact tatactccct actttcaaaa
    13561 taaccatcat catttgatat atatcccttt ataaatctat tattcatatc atcaaatctt
    13621 ctcatagttc cagctacaat atctatatca tactcttttt taatcaattc tgaactagat
    13681 tttatattta gactatctat attaagtatc ttatttatat ctctataact agcaaacagc
    13741 ccatgacaat atggtagtat tttcttataa tcatttacta aatcatcata agtcctttct
    13801 ggatttaatg attgtctaaa attaaaatca aagaatactt ttatcccctt ttcataagat
    13861 tttctagcta gttctaaagc aatcttttta actgaatctg taagttgcat agcaatacca
    13921 caaatatgga ctatatccat ttcttctaaa catttatcaa tatcataatc ttgtaattta
    13981 ctctcacaaa atgaactatt taacctattc aaataagtca cttcagaagg tctttctcca
    14041 tacccatttt caagaaaata cactcctata tgattccctc catatgtgat aaagtcatct
    14101 tttactccaa gctttcttat atttgcactt gatgctttac ctacattatt attaggcaac
    14161 tttgtcaaca tataggtatt gtatccaaag tttttaagtc cagataatat atttaatccc
    14221 gtgcctgtaa ttatataatt tagttcattg gtttgtgtta agaacttgta gtcaggtact
    14281 gaaagtctca tcattatttc accaaaagct aatatattca taaatctatc ccaataattc
    14341 tttcattatt ttataaagta ctcctatatc ttccactttc gtatcacctg tttctttatc
    14401 aattattgat gtataaacat gaggtataac tttttctaca ccagcatcaa gtgcaatttg
    14461 aactatctct ttgaagttat ctaagtctat accccctgtt ggttctaaat aaaaattatg
    14521 ctttgcacaa gcttttgcaa cttctatata ttcatccttt gttttaagcc ctcccattgg
    14581 aaaaaactta attgagcttc ctcccatatc tttcatcata gctattgctg tttctatagg
    14641 aactatacca tctttttctt ttgaacttaa tggacctgtg gatattttta caaatcctgg
    14701 tttaccagtt ggagaaacta atccatttat tatactatca ttttgaccaa gcaatgctct
    14761 agatgtagcg acacctgtaa acacttgatt tatatgttgt ggctgaagtt ctttagaaat
    14821 ctgagatacc atatttgact gatttgggtc tccagctcca agacccacag atattgcatt
    14881 atctatttcc tttgagtatt ttttcatatc ttcaatagct gcttctacag tgctataatt
    14941 ctttgatagt actcctacta gtacatgtcc atcacaggca tcatatactt ctttagcatt
    15001 ttttatgctt cctgcaagaa catttaaaca aactctattt ttataatatt gtggtataca
    15061 attcatattt atcccctcca ttggtaaaat tcaatttatt aaatcattat aatgtcactt
    15121 actctacact ctaactttct actatatttt ttatcctatt ataaatatta tctaaatcac
    15181 ttccagttac acttcttata tcaatctcaa tcttgccttc attagcctta tagtctctag
    15241 tgtatatagc tatctctcca cttctaagtt catttataat ttgttgtgtt gacttattta
    15301 ttatatcttc attaaagtta atttctccac gaagtatttc tctacctgct gaatctctag
    15361 ttatactagc actcactccc tttatagtat ttatcttgtc tataaatgga tttagcttgt
    15421 cctccatttc ttttaaagtt actttatcat tacttatata tctctcaatt gcacatgtta
    15481 gacctacaat attttctttt cctattttca tagctcttcc tatacccttt gattgaagct
    15541 ttacattatc tatgtatttt ttcttaccta ttactaaccc cgagctagga ccttctatag
    15601 ccttagcacc actgtatatc actaggtctg ctcccaattc ataatataca tttaaatctt
    15661 cttctgctgc tgcatctact atcaaaggta aattatattc tttagcaact tgtactgcat
    15721 ctaatacact tagcatactc ttttgaacac aatgatgaga tttaatatac ataattgctg
    15781 ctgtattttc atttatctct cttattatat gttccttgtt acacatatta gcatagccgg
    15841 cctcaacaac ttctcctcct ccaagtctta tcattacttc tactggaact ccataatcaa
    15901 cattatgccc ctttggaatt attatttccc ttttaacttg tttcttagga ttatttacat
    15961 tgtaaactag actcatatca tcttttgcta tcattgctcc cacactctgt gcaatcccag
    16021 cagatgcact agaaactatt agtgcacttt cacaccctaa taaatttgct atatattcac
    16081 ctgttttatt tacaagttct tctattataa ggaaattttt acacccttca cctattgctt
    16141 caataacttc cttatctact gttgatactc ctagtatagt cattttgcct gaagcattta
    16201 taacacgtct tacagagtat ttttcaaata tactactcat acaatagttt ctcccttcta
    16261 ctcattctct tctatagagt tgcctatatt taaatattct ccattgacta ctactgctct
    16321 tggctttata gacgtacttg tgattactga cttattcaaa gaatcttgaa gttctttttc
    16381 ttcattaacc acatcaaata tagttaaatc agcatcatat ccttctttta ttaaaccttt
    16441 attttttaaa cttattgcat ctgctgcatt tttagtaact ttatctatta tatcttcaag
    16501 tgaatagccc atatatataa acttttccat tgttgtagat agattataca ctggaccatt
    16561 tattctattt ttaatatata tatctgtact tatagtgtct ggaaaaattc ctgcatcctt
    16621 agatttcatt gcaatatcca tactaaaact ttcggttcca tgacctacat caagaattat
    16681 acctctttct ttagcttcta tgatttcctt tttaacctca ttgcctctaa gtattccatt
    16741 tggctttcca ttatatatat gggttaatat atctcctttt tccatataat caaatatatc
    16801 ctctatcgtt ggaggagaac tcccaaagtg aaccatcata ggtagattaa gttcattttt
    16861 tattctttta gctactttta aaggttcaac atcatttgaa attacaacac ttttactcat
    16921 tctagctttg attccaacaa caaaatcttt gtattttcta actgcatttt taagttcata
    16981 ttcatcaata ttcatcatat ttgaaagttc atcttgagat gttattccct gcttagcaat
    17041 atttattaaa gcatatacat tggtcttata ctttttagta tcttcataaa attcacctat
    17101 atctaaagct cctgttgttc ctgcatctac tacagtagta actccacttt ttattccaac
    17161 ctcatctgga taatccttat aaaggtctag tctttcgtag cagtgtgtat gtatatctat
    17221 ccaacccgca ctaataaatt tctctccatc taagtttata atttgttttg cctgatactt
    17281 agaatcatct atatgatttt ttacttctat tattttatcg tctaatatag ctatatcaat
    17341 tatactttta tctactgttt ttccacattt tattaatatg tctatcttca ctttactctc
    17401 cctctaaaat aatcttaaat ttactccaag gttcaataaa gtctaaaagt attatttcgt
    17461 cttcctttat tctacccacc ttattttttc tttcatctgt acaatcttga agtatcaact
    17521 gtaattcccc tttatacttt ccaaagtcat cattaccaat cactacatct cctcttttaa
    17581 agatgttctt attatcatgt ttagggaaat tttctttagc atattttttt cttacttctg
    17641 tagatcttgc tacctgatta gttatatctc ctcttctaaa gtgttgctca tttaccatta
    17701 ttatcttttc tacttcagaa gttttttctt tagctactac attaaataca acttggtatt
    17761 catttacttc agacattctt tttaattcat cttcacttgc atatgcattt cctacaatta
    17821 catcatcaat caatcctgtt gcgaataagt gcttagttgc tgtagcaata tctaagtttc
    17881 tatgcatttc aagcgtacac agtccatcat ttatattcca aggtccgatt gttgcgtatt
    17941 ttgaattgac aaaagcagca gttcttattc catacttttt aaatctattt gagcatctta
    18001 caaaaaaatc atatggcaag cctgtacccc tttgaggata aaaattgtga caaccatata
    18061 tattatttac atttggcttg taagtaatta tattatccaa gtatgctaca tcattactca
    18121 tatttagttc cacttttaaa tcatatggat tgaaagaaat catagcttct ttacttccat
    18181 catatccata gtcaagcctt attccactag catttaactc actaaagaat gttaaatcat
    18241 cataagatat ttgtaatata tcaaatatgt ttggagctat atcaaggatt acttcaaatc
    18301 ctaattctct tgcataggtt ataatctctt taaaatgttt tttaacttct tccttaggtc
    18361 ttgtaattga aagcatgcac ataaatattc ttttaaatcc gtattttgaa accattttta
    18421 tatatgcctt atcatcttcc atgttactat gctctggata tactgatatt cctaatcttc
    18481 tttccatctt tctcctcctc aaattacatt atttaaaata tataaaatta aaggcaaagg
    18541 tcattcctcc taccttcacc cttaataact tataagcttt ttctatattt tatatagtac
    18601 aaaacctcta tatctatgta tttttataaa ttttcactca tttcttcttg ttctctttgt
    18661 aattgttgtt tttcaaaaac tttgaagaat ggataataaa cagcaaatga tatcacaaag
    18721 ttgattaata ctaagatacc tgctagtata ctccagttag tactcattac tgctgctatt
    18781 ggtgctggca ttgcaaatgg taatcttgct accatcatag gtataacccc tgtaatagta
    18841 agaacatatg atagaattgt cattattata ggtccaatta taaatggtat ccctagtatt
    18901 ggattcataa ctattggtgc tccaaatatt ataggttcat ttatgttgaa taaccctggt
    18961 aaaaatccta atctacctaa actttttaga tattgtgact tagaaaacat aaacattaca
    19021 actaatgcaa gagtagcccc tgctccccct atccatacat accactgtaa aaattgttct
    19081 gtaaatatgt ttggcattgc atgagctgat actccttctg tgaatgcagt tatattttct
    19141 gctatagaca tgtcccatat aggtcttatt actggcccca ttatagctgg accatgtatc
    19201 ccaagtaccc agaataaagt tattaggaat actgtcagta gcccaccacc taaactattt
    19261 cctgataaga atccttttaa aggtgataac atagcgctta gtgcaacact tatatcaaat
    19321 cctaaaatat gtcttattac ccagaataac attaaaatta ctgctgtcgg taataatgca
    19381 gtaaatgaat ttgaaacctc tactggaact cctgctggca tcttaattgt aatattcttt
    19441 tccttcataa aacgatatat ttcaactgta attaatgatg ttacgatagc tccaaataat
    19501 gatggcgaat taagttttgc catatccatc catctaccag cttctataac tccacctact
    19561 ggttcaacta cttgagttgg aactattgta gaaactaaaa atgccataac tgcaagcata
    19621 ccaacacctg ttgtatctaa atcgtagctt ttagcaagtg atgatgctat cccaaaagta
    19681 gcatatagag ataaaaatcc tacagtatat ctaaatggta tatctaatgt tgctttaaat
    19741 ggagctataa aatccatata agcatcaata ggtatgttta ataatattgt gaaaaatgac
    19801 cccactattg ttagtggtaa tgttgctatt atacctgctc ttacagctct catatgtctc
    19861 tgattactta tcttcgctgc tacaggcata atcttgcgct ctaaaaaact tgcgaatttt
    19921 tccatagtta tccccctgta ttattgatat tatcctttat ttataatgca ataacgattt
    19981 cataacataa actaattttt atgtaattga taatatcact tatttattaa aaatattata
    20041 acattataat gtttggttgt aaacattata atgttataat atttttcgtt tttacaacta
    20101 aacaaaaatc ctgagcatat tatcatttca ataaactcag gattcttatt atatttcgta
    20161 tttaattacg tagttttgta cttctgtatt atatcttgca aatgatagct caactatatt
    20221 atcattctca tcataggtct ttctttttct tcctaataat atgtctttag ctagatttag
    20281 agcctttgat acctctagtt ctgattcttc tataaaaaat tcatcttcaa atctagttat
    20341 atttatattt ctcctatata agtacatata taaagaactt tcattattaa aactttcaac
    20401 agttagattt tcatcaaaag gaatataatg agtaaaaaat atatatggct tactatctaa
    20461 gtaatacatt ctagtaatct tgctacattt ttctccaaat atactaaata cttgtgaatt
    20521 tgtatcattt attatttttt caattgaaat agtcttttta gataacttat gacctttaga
    20581 taaaagtata cttgaaaaag actctgcttt tgatagctta ttaaaaagac tattacttat
    20641 tacttttgtc ccttttccac tttttttctc tacataccct tctgattcta atatctcaat
    20701 agcttttcta atagtaattt tactgactcc aaattcttgc tctaattctc tttctgttgg
    20761 aatattttca tcaattttat attcgttatt taatattttt tgctttaata tatctacaac
    20821 ttgtatatat aatggttgat ttcttaacat gattcacctc ttaaattata tgttataata
    20881 ttataacata taatcttata ttattaataa taaatattta ttaactttaa actaaatata
    20941 aaaagagtcc tcaaaataat cctaatgtat tactcactaa atattgaatc aataatctaa
    21001 caattagtgt gctacacata atgtatcttt taagaactct cttaatttat tctctttaat
    21061 ttttatttat cttgtaattt caaatgtaaa tcaacgattt cttcagctaa ctgttgaaaa
    21121 ttcataccat tcattaggtg gtcctgtgca tgtataagta tgacacttat ttcagtcctg
    21181 tttccactag cttcgttttg tattaattct gtttgatatc tatgtgcttt atttataact
    21241 tccttagact cttttaataa ttcttttgct ttttctatat ttccattctt agcctctttt
    21301 attgcttcaa aagctaaccc tttaccttca cctgcataac ctattatatt aaaagatatt
    21361 tctataatct tttcatccat cctttgattc tcccaattta tatattaaaa atacattata
    21421 atttatatta cttagtgttg ttaagttcta tcgctctatt taaaacagct tctccattaa
    21481 ctgttccata gtgcatcata tttataacct ctactggtat attatatggc ttagcaattt
    21541 cttgagcctt aggtaataca tatctaactt gtggtcctag taataataca tcacattttt
    21601 ctatttcatt ttgtatagtt gattctggta ttgcccaaat ttgcgactct ataccatttt
    21661 ctttagctgc actttccatt ttagtaacta ataaacttgt agacattcct gctgaacagg
    21721 ccaacattat ttttatcata tattttcctc caaattttta ttaacaacta tataaacatt
    21781 ataatgttat aatattttat ttaattctac cttaaatgca atatactttc aatacttttt
    21841 tgttatttta atattattaa gtgtcaaatt agcaaaaacc gtatcattta tttatgatac
    21901 ggttctccat tatttatcct ataagcccta taaatctgtt ctaacaatat caatctcatc
    21961 atctgatgag gaaaagtcat cttagaaaag gataacctat aatcagccct tcctaaaacc
    22021 tcatcagaaa gccctaaaga acctcctatt ataaaacaga gactactatt acctctaact
    22081 gctaaatcac tcatagtctt agcaagttct tcagaggata aacccttacc atcaatagcc
    22141 aagactataa cataactatt atgctttatc ttccctaaaa ttttctttcc ttctttgtct
    22201 tttataattt ccatttcctt aacactcaaa ttctctggag ctttttcatc atccaactct
    22261 ataatttcta acttacaata cttagaaagc ctttttttga actcatcaat acctaatttt
    22321 aaatattttt ctttaatttt acctactact actatactta tattcatata tttctcctaa
    22381 acttaatttt attacaaatc tatgcattac tagaatttac aagctcttat aaaattatat
    22441 ctctttttct tgatttgcat attctacaaa ctcttcaaaa ggtattgctt tgctataata
    22501 atatccttga tatatttcac atcctaaacc atataactta tccctttggt ctatattttc
    22561 aacatactca acaactatat caaaatctaa attttttgaa agatttacaa tagtcattat
    22621 aatttcacta cttcttttat ttgtaagtat ctctttaact aatgaaccat ccaatttaac
    22681 tatatcaaag ttattatttt gaagatatat aagagagcta tgccccatcc caaaatcatc
    22741 cataattaac tttattccca agttatgtat cgcattgata cgttcaataa ttatttgtga
    22801 acctgataat gcaatttgct ctgttatttc tatacctaac atattaggat ttatatcatt
    22861 actactaatg atatttttta tttgctccgg taacttaggg tcatcaagtt ggtctggaga
    22921 tatatttaca gagaacttaa caggatttct caatagcttt tcactcttct ttaaatcaat
    22981 acatgcttta ttaataatat actctccaag ctcatctaag aacccttctt ctttagccaa
    23041 aattataact agtggtggat aaatgaaccc tcctattgaa tgtttccatc taagtaatcc
    23101 ctctgctccc acaacatttc cattataatt gacttgtggt tgataaaaaa gttctatttc
    23161 tcctcttttc atagcaaact tcaagtctga tgccaacatt tttgaaattg aacctatttt
    23221 accattatgg tttagaagat taggctgttc acctgttaat tcataccttt ttaataagtt
    23281 tgttaaattt tctatattat tcttaaacat atatgtatat ctattttgcg acatttttat
    23341 aaacggaata taaatcataa caccaacaca taaattaaat acttgcaaca aactacctct
    23401 tattgaacct gtagacatat atccacttaa aaatataggt gaggtccatt ctacagctga
    23461 aactgtacat ggtacaatac ctgtagacat agccacataa cttgtaatgg ttaagattat
    23521 aggagtaatt acaaaaggta caaacataat atagttaaaa acaattggta tcccgaaaac
    23581 tagcatctca ttgatattaa aaagagctgg tattgaagca attttagcta gctttcttac
    23641 atttatacgt ttttcactaa gaaaaattgc aattactaaa catagtaaag agccacatcc
    23701 tcccaataaa accattgtat caaaaaaggt cttagtaaaa atttctgttg gtaattgatt
    23761 attattaact aaatttatgt taattgccat actattttca aataaatttt ttgcgacggt
    23821 atctaataca ttactaccat gaatcccaaa aaaccacata aagtgcatta aaaaaataaa
    23881 caacagagca ctcataaggt ttgttcccat tttattaaaa acatttgaaa ataaattata
    23941 aaatatatct tgaaagtttg aactgccaat tagacttatc attatcactc taaaaataga
    24001 aaacaaaaat actactatta tgaaaggaac tatagccgaa actaccatat taaagtctgc
    24061 atcagcccct tctgtatgaa actttacact tgtaacaaaa gattctgtaa gttttacaaa
    24121 caatacagaa gatgtcatag aaacaattat agatgtaaat aaccactttg tcttaaatat
    24181 ttctatataa gaattaagct cattaccatg tgaaaatact aaaaaagagc acattgcaac
    24241 tataggaaca agtactgtat acttgctccc atatattttt ccataactat agcttattgt
    24301 taataatatt attaatgaaa taattcctaa agtagaatca tttataaagt taagaatatt
    24361 tgtaacctct ccattaaaca agcctgataa atattcttga taagccttta ttggaagatt
    24421 caaaattaca agagccgttg aaccagtcat aatagcaggt attgctaaaa tcataccctg
    24481 ctttatagag tataaaattt tattatcctc aacattttca aaatttctct ttagcaaagt
    24541 tattcacctc tacttcatct cataaacatt agaaacatca tttcgtgata gtacatctag
    24601 ttttacatct tgtccaacta ctatatcatt tgatgctaat actccttttg aagtctcata
    24661 tgctaactca ggaaaattat tttctttact taaatgtgct aataatatag attctgtacc
    24721 ttcattaata agttcaacgc agaaattagc tgcatcttca ttggataaat gaccagtact
    24781 tgacattact cttttcttta aagcatatgt atatgaaccc atcataagca tattagggtc
    24841 ataatttgat tccaatacta ctaatttaga tttatataga tgttttttta tattttgagt
    24901 tatacatcct atatcagtag ctatagacat tttttcatca ctctctgtat aaaaattata
    24961 tccaactgga tcagacgcat catgttctat tgaaaaaggt ctcactatga tatcacctaa
    25021 tgagtaggtt ttatcatttt caaatacttt catattttta tctttgatgt cacctaattt
    25081 gtccttcata gaacaccatg tttttatatt agcaaatact ggtatatcga actttctgga
    25141 taatatacct gctcctttta tatggtcaac atgctcatgt gttataaata ttccttttaa
    25201 tttatctgca tctacaccaa tgtctttaag acctgtagtt attctcttgc cacttaatcc
    25261 tgcatctact agtatattag tatttttata ccctatatag tggcaattcc cactactacc
    25321 actccctact gaacaatact ttagcattcc ttcacctctt tttctgcctt ataaattata
    25381 ttataccaaa aaaacagagt attaaataaa atttaatact ctgcacaaaa tttacttaca
    25441 ttcactattt acttacattc acttataatc ttatttatat ctacaccttt actattccat
    25501 agttccacaa aagctcttcc attagcattt ttatgcttat atttattata gtattcattt
    25561 aaagtctcaa agaatacttt atctccaact tcttttctta tttcattaaa tgctattgcg
    25621 ccttttgtat aaacattcaa gctgtattca gtagaatttt tatactcatt tgctggctta
    25681 aatatatttt cactcaaata actttttgtc tgtatctcca tagtttttat aagcttgttt
    25741 gccatatctt taccatactt ttgttcaaaa taaactattg tagaatattc agtaagtgct
    25801 tcatctaacc agggttcact tatttcatca tttccaacag cagaatacca ccattgatgt
    25861 gctgtctcat gggcaataac atattctagt aaaaattcat ttttttcatt atataaactt
    25921 tggtctatca taacaagcat agggtattcc atccccccta taaaaaagtc acttgctaca
    25981 acagaaaatt ctttgtaagg atactttcca aataattcac taaatatatt tattgaatct
    26041 tttgccactt cagttgcttt tttagaaagc tttttattta aattatatgt attgatagct
    26101 actccctttg aatctacttt gtttacatca aatttactac ttagtataaa tgcaaaatct
    26161 ctaacgtttt cagcttctat ttcatatagc attttcttat tatcatgttt atcatcaata
    26221 agttttcctg tatgagcaat tttatattta tttggaatca atagtttcac atgaaaatcg
    26281 ctagtctcac tataaaatgg gtcgccaata gtttcataac tctttaaatt ccatcccctt
    26341 tcatcattta cacaagctat aggaaaccaa ttagtaacat ttatagtatt ttctccatat
    26401 ccaaatcttc caattgaatt tggtattttt acgttatatt ttatatctaa tgacattctt
    26461 tcatttggtt ttaactcttt ccctaattct atttctaata tgtcattttt gtctccttta
    26521 attttatact ccattttact attattattt aatatattct ttatatctat atatccttcg
    26581 ttaaatccat taggataagc ccttgtcatt tcactttttt caaaaggagc aaaatctttc
    26641 ttggaaaaag cattcggata tatatggaaa tatattttat ctatgtttga ttttgtatta
    26701 tttatgtagt caacattctg attgcacatt aacctcttag tctcatcatc aaatataaca
    26761 tccatattat actgatttat ttttgaatta gttttttctt tgtatgtttc taaactgtca
    26821 tccttttcta taaagcaacc tattattaat accataaata gaatcgctac tgataatgtt
    26881 cttcttactt tttggttgaa ttttataacc aaaatatctc ctccttttca aaatgaatac
    26941 ttttagaaaa tatttacatg ctaatcttgt gaccttttat ttgtttacag acaaatataa
    27001 tataaaaaat gatataattt aatctgaggt gataaaatgt tttttaaaga aagcaatgaa
    27061 atagatttag gtgaaatcac tattcccaat atctttatag atatatttat gccaatggcg
    27121 gacgggttat acgtaaaagt ttatctttta ggatatagac aagcatgtga tattacttct
    27181 aatcctaagt tcgacaataa ttcaatagcc aaaaacctaa atataccatt gtctgatgtg
    27241 ttatctgctt ggaaattctg ggaagaaaag aagattataa aaatacatga caatggagaa
    27301 tatgataatt ttaattattc aatagaattt ttagatttaa aaaatttcta tattgagaat
    27361 atattaagca ataattcatc tataaaatct aatacagata aggttgtatc tacttctgaa
    27421 aatccaagta ttagaaaaat gtttaattct ataaataaaa tagttgggcg atatctcgac
    27481 ccaagtgaaa agatgagtat tatggatata atgaataaat acaatatgag ccccgatatg
    27541 atactttgcg catatgaata cgtcaaagat aaaactggta catcaaagcc agttaaatac
    27601 attgaaggca ttattagaaa ttggtatgat tcaaatttat atacgccaaa agatgttgaa
    27661 gaaagctttt tggtaagaag tgaacgatat attttatata aaacgatttt caatgaattg
    27721 ggattttcaa gacaaccatc aaaatcagaa aaagaactta tggacacctg gtttgataaa
    27781 tttaatatgg atatagattt gataataaat gcatgttcta agtctaagaa tatttcaaac
    27841 ccaagtatat cttatataaa tggtataatt aaaaattgga atgaaaaaaa tataaaaaat
    27901 ttaaatgatt taaagcaaaa agaggaagaa cgtatagtta aggagaatat taataagaag
    27961 cagattaata ctactcaaaa caacaatact tacaaaaaaa ctaagtttca taatttcaac
    28021 gaaacattca ctcagtatac ctctgatgag ctcgatgaga ttattaaaaa aagtcaaaaa
    28081 gaaaagttta agtaggtgat taaatgaatg aggataaaat aagaaaaata cttgctaaat
    28141 atgctaagag aagagatgat aatgaactct tattagaaca tagaaaaaat gaggtatata
    28201 atagaatccc tgagataaaa tctatagatg atgaaatttc taaaataggt ctcagtttag
    28261 ctaagatagt acttttaaat cccaaatcta aagatgaaat tgtaaagaag actaaagaga
    28321 atatagagtc tttaaaagta aagaaagaaa aacttttatc tgaaagtaac atacctcttg
    28381 attacttgga aataaagttt caatgtattt cttgtaaaga taaaggattt ttacctaatg
    28441 gagaaaaatg ttcttgcctc aaacaagaaa ttgtaaatga agcttataaa atgtctaatt
    28501 tagataggat tttaagtcag gaaaattttt cgaattttaa cttgaatata ttttctccta
    28561 agaaaggctc tgatggggaa atatctccta gagaaaatat gctaaataat ttaagtatct
    28621 gcgaaaattt tgttcatgat tttaagaagg ataatagtga aaatctactg ttttatggtt
    28681 ctactggact aggaaagacc tatatgtgca attgtattgc aaaggaattg ttagataaag
    28741 gaaatgttgt aatctatcaa acttctttta gaattttaga tattcttgaa gattataaat
    28801 ttagaagaga cacaaataac caaataagtg aagataatta taaaaacttg tttgattgtg
    28861 atttattaat aatagatgac cttggaactg aacttaataa ttcttttact agtggggaaa
    28921 tcttcaatat tgtaaataca agacttgttg ctggtaaaaa aatcattatt tcaaccaact
    28981 taacaccatc tcaaatagga aatacttaca ctcaaagaac tctctctaga atcttagata
    29041 agtttaggat tttagaattt actggagatg atttgagatg ggaaagattt aaataaaata
    29101 gcccttaaaa aacatttttt tatttttaaa tagagaggta agagcaaact tgcttctctg
    29161 tttaaaactt atgccacata aattaaaaca tattttttat ttttctattt acatatctaa
    29221 tgtatcttaa tctaacaaat catttacatt cttagtagtc tatatttatt gatactataa
    29281 attaaatttg ctatattaca taatactatg ctagaatgat tctaaatttt atatttaaaa
    29341 ctatcacatt gcaagtaatt caaaattttg ttgtatgttt aatttcattt ttaata
//
LOCUS       DAELSY010000031        29017 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.031, whole genome shotgun sequence.
ACCESSION   DAELSY010000031 DAELSY010000000
VERSION     DAELSY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29017
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.031"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            110..994
                     /locus_tag="KPG09_001296"
     CDS             110..994
                     /locus_tag="KPG09_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG0965917.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            1093..2268
                     /locus_tag="KPG09_001297"
     CDS             1093..2268
                     /locus_tag="KPG09_001297"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBG0965918.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEELLKDIPTANKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            2396..3250
                     /locus_tag="KPG09_001298"
     CDS             2396..3250
                     /locus_tag="KPG09_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965919.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSNESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKIKDDSSC
                     LYLDIYND"
     gene            3294..4529
                     /locus_tag="KPG09_001299"
     CDS             3294..4529
                     /locus_tag="KPG09_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBG0965920.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREIVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKSISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYISLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNEKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            4630..5571
                     /locus_tag="KPG09_001300"
     CDS             4630..5571
                     /locus_tag="KPG09_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBG0965921.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(5800..6153)
                     /locus_tag="KPG09_001301"
     CDS             complement(5800..6153)
                     /locus_tag="KPG09_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG0965922.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMIW
                     LEKLKEDNTYEIYSI"
     gene            6449..7360
                     /locus_tag="KPG09_001302"
     CDS             6449..7360
                     /locus_tag="KPG09_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBG0965923.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            7620..8054
                     /locus_tag="KPG09_001303"
     CDS             7620..8054
                     /locus_tag="KPG09_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965924.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            8077..9612
                     /locus_tag="KPG09_001304"
     CDS             8077..9612
                     /locus_tag="KPG09_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965925.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVIYK
                     IDCDVILVNLFELIINNCIFSTILGNEVINLEISKYEYEFLEKQFKNMYNNITEDKVI
                     GSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKTEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            9696..11063
                     /locus_tag="KPG09_001305"
     CDS             9696..11063
                     /locus_tag="KPG09_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0965926.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKARDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMNTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            11098..11322
                     /locus_tag="KPG09_001306"
     CDS             11098..11322
                     /locus_tag="KPG09_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBG0965927.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(11772..12170)
                     /locus_tag="KPG09_001307"
     CDS             complement(11772..12170)
                     /locus_tag="KPG09_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBG0965928.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMVG"
     gene            complement(12173..12574)
                     /locus_tag="KPG09_001308"
     CDS             complement(12173..12574)
                     /locus_tag="KPG09_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0965929.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLVAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            12933..13919
                     /locus_tag="KPG09_001309"
     CDS             12933..13919
                     /locus_tag="KPG09_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0965930.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLNEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            13903..14814
                     /locus_tag="KPG09_001310"
     CDS             13903..14814
                     /locus_tag="KPG09_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0965931.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            14927..16495
                     /locus_tag="KPG09_001311"
     CDS             14927..16495
                     /locus_tag="KPG09_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0965932.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHVLEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            16570..17595
                     /locus_tag="KPG09_001312"
     CDS             16570..17595
                     /locus_tag="KPG09_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0965933.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDEYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            17588..18532
                     /locus_tag="KPG09_001313"
     CDS             17588..18532
                     /locus_tag="KPG09_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0965934.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            18891..20549
                     /locus_tag="KPG09_001314"
     CDS             18891..20549
                     /locus_tag="KPG09_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0965935.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERDSKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASNGKSSW"
     gene            20530..21546
                     /locus_tag="KPG09_001315"
     CDS             20530..21546
                     /locus_tag="KPG09_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0965936.1"
                     /translation="MVKAVGDIIGKCSAISFDMKLDKSYDEVIVDFKNLINNIDNKDV
                     LLFTDMGSLNSFDEIIKKEKKCEVRVIPMVTTLTVLEAVQKANMGLPLNDVYNSITNT
                     RKYYFGTNEIQNKENLSKTIIIASHVSEGVDNKTRKILEEKMSRYLDGIDIISVPYKT
                     EKDLSLNITKLKESSNIVAVINEQRINIRGIDYISKKDIDKDENINKLKNIIKISIGY
                     DDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLVFNIEKKYDKVIGLMMHLAFMVDGLI
                     GNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDKKYNIEINEKECYQILLILEYAEIIE
                     KDYQ"
     gene            21761..22390
                     /locus_tag="KPG09_001316"
     CDS             21761..22390
                     /locus_tag="KPG09_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965937.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            22525..22851
                     /locus_tag="KPG09_001317"
     CDS             22525..22851
                     /locus_tag="KPG09_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0965938.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            22867..24156
                     /locus_tag="KPG09_001318"
     CDS             22867..24156
                     /locus_tag="KPG09_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0965939.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            24180..24509
                     /locus_tag="KPG09_001319"
     CDS             24180..24509
                     /locus_tag="KPG09_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0965940.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            24657..25985
                     /locus_tag="KPG09_001320"
     CDS             24657..25985
                     /locus_tag="KPG09_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG0965941.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            26302..27162
                     /locus_tag="KPG09_001321"
     CDS             26302..27162
                     /locus_tag="KPG09_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBG0965942.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            27367..28347
                     /locus_tag="KPG09_001322"
     CDS             27367..28347
                     /locus_tag="KPG09_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0965943.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVICGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
     gene            28550..28999
                     /locus_tag="KPG09_001323"
     CDS             28550..28999
                     /locus_tag="KPG09_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965944.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARVLMFKTYHMLIYIKEKYYYVNIY"
BASE COUNT        11634 a         2927 c         5140 g         9316 t
ORIGIN      
        1 ttttaaaata tataaatatg tattgacata taaatattat gaattacaat ttatataaac
       61 taataaaaat taaataatat taatcaactt attgaatgga ggaataaaaa tgaaatttgt
      121 gactttttgt agtagcaatg aagaaaaaat aggtgtattt aattcagaaa caaatagtat
      181 atatgaaatt aatagtttag gattaagtaa attatatact gatatgaatg attttattga
      241 aaacgtatct acaggagatt tagaaaaaat aaaaaacaat tcatttgaaa acgcaaagtg
      301 ttataagtta gaagaagtta aattatgttc cccaattgta agacctaaaa aagatattat
      361 atgtttaggt ctaaactata aagaccatgt caatgaaatc ccagatggag ttataaaaaa
      421 cgtagttatg ccagattatc caatttattt ttctaagaga gcagacaaaa taattggagt
      481 ggatgataag ataagcttac atggagattt ggtagagaag ttggactatg aatcagaact
      541 tgcagtaatc ataggtaaag aaggtataaa tatatcaaaa gaagatgctt atgaatatat
      601 atttggatat acaatagtaa atgatataag tgaaagagca cttcaagata aacatgttca
      661 atggtttaga ggaaaaagct tagacacaca tacatctatg ggaccttgta tagtacataa
      721 agaagaattt gaacatcctt taaaattaga tataagtagt gtagtaaatg gagaagtaag
      781 acaaaattct aatacagagt actttatatt tgatatacct actataataa atgatttatc
      841 aagaggaatg actctaaagc caggagatat aatttcaact ggaactccag caggggttgc
      901 tatggggatg aatcctcaag tatatttaaa gcatggcgat atagttgaat gtaaagtcga
      961 aggaatagga gttttaaaaa atatagttga ttaatggata aatatgccat ataattttat
     1021 tagtacaaat aatttaatta atataggatt gatcaaactt atgattaatt tattatagag
     1081 agggatgagt atatggcaag agaactagaa ttaaagtatg gctgtaatcc aaatcaaaaa
     1141 ccatcaaaga tttatatgaa gaatggagaa cttcctatag aagtattaaa tggaaaacct
     1201 ggatatataa attttttaga tgcatttaat agttggcaac ttgtgaagga attaaaagaa
     1261 gctacaggtc ttccagcagc aacttcattt aaacatgtaa gtccagcagg agcagcagta
     1321 ggggtacctt tatcagatac tttaaagcaa atatattttg ttgatgattt agagctttct
     1381 ccattagctt gtgcttatgc aatggcaaga ggagcagata gaatgtcttc ttatggtgat
     1441 tttatagcac tttcagatgt atgtgataag gagactgcaa ctattatagc aagagaggtt
     1501 tctgatggta taatagctcc aggatataca gatgaagccc ttgaaatatt aaaaggaaaa
     1561 cgtaaaggca actataatat tgtaaaaata gatgaaaatt atactcctga gcctattgaa
     1621 acaaaggatg tttatggaat tacttttgaa caaggtagaa atgaaatatt aataaatgaa
     1681 gaactattaa aagatattcc aactgcgaat aaaattttta cagatagtgc aaaacgtgat
     1741 ttaattatag ctcttattac attaaaatat acacaatcaa attctgtttg ttatgctaaa
     1801 gatggacaag taataggggt aggtgcaggt caacaatcac gtattcattg tactagactt
     1861 gcaggtaaca aagctgatac ttggtatctt cgtcaacatc ctaaagtatt aaacttaaaa
     1921 tttaaaaagg atataggtcg tccagataga gataacacta ttgatgtata tctttctgat
     1981 gattatatgg atgtgcttgc tgatggcata tggcaaaact tttttgaaga aaaaccagag
     2041 cctctaactg gagaaggaaa aagagcatgg cttaaaactt taacaggagt agcacttggt
     2101 tcagatgcat ttttcccatt tggagataat atagaacgtg caaaaagaag tggggtaagt
     2161 tttatagcac aaccaggtgg ttctattcgt gatgataatg ttatattaac ttgtaataag
     2221 tataacatag tcatggcatt tactaaaaat agattattcc atcattaaaa tactgaaagt
     2281 ataaagataa tatagattaa gtaaaaatct acaacaagtt ttgttgtaga tttttttatt
     2341 tgctgtataa ttttaaatag aactttattt ttagatattc aatttggagg ttgatatggc
     2401 tttaaatata agaaactata gcctattatg taatcaagga acagctaaat ttaatgaaga
     2461 tgtagtcggt ataaatccat ttggggcatg ggttttagat ggtgctacag gacttaatgg
     2521 aaaaaattta gtgtcaaatg aaagtgacgc aaggtggtat gttcagtggt ggaataggta
     2581 tttatataaa aatataaata aagaagaatc attaaagagt attattaata ctggaataat
     2641 agaagtaaaa aaagagtatg aactaagatt aaatgggttt aaggttgaaa aattagattt
     2701 accatctagt tctatagttg ttgtaaaatt tcatgaaaat aaagtagagt attttttatt
     2761 aggagattgt gctttgttta ttaaaaatgg agattccaaa gttataaaag ataggagtat
     2821 ttgtaaattt gataatactg tatttgatga aatgagtaaa ctttctaact tggaagagat
     2881 ggcatttgat gagattagaa ctagtgttat ggatacaata gtaagcaata gattgaagaa
     2941 aaatacgaaa gagggctatt ggatattgga ttttgatgag gaagctgtgg aaaattctat
     3001 atatggatac attgaaataa aaaaagattt tcaattgatg ctaacgagtg atggttttac
     3061 aagtgcatgt gatagatata atctaataaa agaagaagaa ttgatacgta tagcagaaaa
     3121 atttggtata ggatatatac ataataaagt aagggatttt gaagataatg actataaagc
     3181 ggttaaagtg ccaagattta aaataaaaga tgattcatct tgtttatatt tagatatata
     3241 taatgattag actgtataaa actatttaat tttagaaagg aggcaagtga aaagtgaaaa
     3301 ttcttcatat aataacacaa aaaccaaata gtacaggtag tggtatatat ttgtctggta
     3361 tgataaaagg gttcgaaaaa atcggtcata agcaagctgt aattgcaggt atagatgtaa
     3421 atgatgatgt aaattgtttt ccaagtgaag taagttttta tccagtaaag tataattgtg
     3481 gggagttaaa ttttcctgtt gtaggtatga gtgattctat gccatatgaa agtacaagat
     3541 ataaagattt aaatatagac atgataaata gattgaaata tcaatttaaa gtaaatatag
     3601 ataaggctat gaatgatttt aaaccagaac ttataatatg tcatcatttg tacctcttaa
     3661 ctgcatttgt tagggaaata gtaaaagata taaaggttat gtctatttgt catggaactt
     3721 gtttaagaca gttaaatacc attgatttag agaaagaata cataatagca aatataagga
     3781 aattagattt aatatttgct cttcatgaaa atcagaaata tgatattata aagacttttg
     3841 gagtaagtga aagtaaagta gttgtaattg gtagtggtta taatgatgat atattttata
     3901 ataaaaatta taaaataaag gatgataaaa taaaaatagt atttgcaggt aagatatgta
     3961 aatcaaaggg attgattcca tttataaaat ctatatctaa attaaaatat agtaaagatt
     4021 taatagaagt caattttgca ggtactggaa gcgatatcga atcatataat gagatagtta
     4081 aactggcaag taagtctcca tttaaaatga atttcctagg aaagttagaa cagagagatt
     4141 tagctgagct attcaacagg tcacaaatat ttgtgttacc atcattttat gagggattac
     4201 cagttgtagt tttagaagct ttatcttgtg gcacagatgt aatcactact gatattcttg
     4261 gtgttaaaga gtggattggt agtgagatta ataattctgg aaagatagaa tatatttctc
     4321 taccttttat ggagaaagaa ggtattccaa aagatgaaga gttatatgat tttgaaaata
     4381 atttgtataa tgcaatagat tctaagatac aatctttatt gaataactca aatgagaaaa
     4441 catctgtaga tatgtcaaaa aaaacgtggg atggcttagc atatagaata aatgaagtta
     4501 ttttaatgga tgaattgtgt ttagtctaaa attatttaaa ttataaaaaa gtgtaactat
     4561 ttttaaactt tttttaaatg attttatatt atactaatac taaatatatt tgttttaggg
     4621 aggtaattta tgaggattag taaaaaggta ttggcgttag gtatttcagc attactgtta
     4681 agtgttagtt ttccaacatc aataaacgca ctagataaga ttgaaaatat tcaaggagta
     4741 gataaatatg aaacagctgg gcttatttca gataagcaag attttacaac agcagttctt
     4801 atcaatgcag attctacaat ggtagatggg attgctgcaa gtggtcttgc aggagtaaat
     4861 aatgcagcta ttttattaac taataaggat gatataccag aagcaacact tcaaagactg
     4921 aatagagtta ctaagatata tgtaataggt ggagaaaatt ctataagtaa agatgtagaa
     4981 aaaatgttac ttatgagaag aatgcaagtt ataagaattg atggtgtaga tagagtagat
     5041 acaagttata aaattgcagg tgaaatagaa aaaattaaaa acagtgataa gatgttttta
     5101 gtaaatggat ttaaggatga agctgatgca gtaagtgttg catctgttgc ttatagagat
     5161 ggagctccta taatattgac aaaagatata ccttcagcag aagaagacac agacttagac
     5221 ccatggtttg gagtttctcc tgttccagta tatgctattg gaggagagtc tacattaagt
     5281 gattacatag tgagcagata tagagcaact agaattggcg gtgttgatag atatcaaact
     5341 aataaaaatg ttattgaaaa gttctataat ggagcaaaag agttctatat aacaagtgga
     5401 gacgatttag tttatgcttt agttgcatca ccacttgcaa aaaatgctcc tgttgtttta
     5461 gtttcaaata aaagtgataa gagtatatta agtggagcta gtaaagtaac agcaataggt
     5521 ataagtgata agagtattat agagcaatgt ttggatgctg taaagaagta atggagtgtt
     5581 attagtaaaa tgaatatata cattgaaaat tagtagattg ttcattttat aatggtataa
     5641 aatatatggg aagaggctat caaatggaga taagcctctt ttttattgat aaaattatga
     5701 aaatatactt tattttatat aaaaaaagta tctcatgaac taaatgtcta ttttgatata
     5761 ctatctcctt ttcttatatt atatgtatta tatgattttt tatatggaat aaatttcata
     5821 agtattatct tcctttaatt tttctaacca aatcatctct aaaatatcta gttcttctaa
     5881 atagtctttc tctgttttgt tatcatcatg ttttataagt tctaaaactt caaatgtaaa
     5941 tgctttttca ccatattttt tccattcatt atttaattct tttatcatac atgagccagc
     6001 accaagctga aatttgagag agttaaattt gctttttatg tcattacttt ttccaaggta
     6061 aatcttttta gtaatatcat tttttattat gaatacacct gtgtcaaatt tcatttcttt
     6121 gtataattgt tttaattcac gttttctatc catgatttta gatacctctc tttataattg
     6181 atatgtaata tgtaagttta aaaatatatt acatatcaat tataaagaga ttaattttat
     6241 atgtaaatag tttttataaa tttaattatg aattagtcat caataaacaa gaaatttagc
     6301 tgtagaaatc cattataaag gctattataa attttaatat gctaaaaata taaaaagaat
     6361 agttgacagt atagaaaata tggaataaac taaaagtgta atagtaatac gaaattatat
     6421 attacattga aaggtgtgaa tgattattat gaataaaaaa atagaattaa tagggaattt
     6481 tgcaagtgat tggattaggt atagtgatta tgaatataaa attactgaag aagatgaatt
     6541 atatatagtt ccaacagaag atgctgtgtt taccatgtat aacccatttg atgtagcaga
     6601 tgatataata gtagatataa ttcgcatagg acaagaggca ttacaagata tatcaaaaga
     6661 gacaaaagaa atgaaactaa agaaagaagt acttgaattt gtaaaaaagt atggattatt
     6721 gggtttaata tattcaagtg tatataatag agatatagtt ggagaagaaa gagtactgat
     6781 gattgaaaag aattatatta caaaagagaa tatattgagt acagatgaat atgtaaacat
     6841 gtttatacca tttgcaagtg atgaagatgt gtattttaaa aaatatagaa ggggtataga
     6901 tgtatcaaag agagaagaat ctccaaagtt ttttggaaag agaccattga ttttagattt
     6961 agtattttca aagttctaca ctgaacaact agattggatt gtaagttttg ctaaagatat
     7021 gtgtaagcat tttaatcaaa tttgtgtata taaaaataca tctaatctat taactgaaag
     7081 tgttactatt atgtctggag ggtttaatcc tcaaaagata ggattttcta taagccaatt
     7141 agataaacca attattgcat gggagtttga ttctttgaaa acagctattg agactatcta
     7201 tgcctttgca attacaagtg aaccttcttc tttaaataaa tgcaagtact gtggtaaaat
     7261 ttatattcct acaaattcta aatctcaata ctgttcacct tcttgtagaa attgctcaaa
     7321 tgtaaagaaa agtagaagta gagccaaaaa agataaataa aattaataaa tatatcattg
     7381 tttagatttt agtaaataac tactgaatta aaagttgtta tagaaaatta tatatctaaa
     7441 tgatggtatt tttttgttga gaaaattgaa aagtttaatt gagatagtaa ttttgagttg
     7501 acaaaaacca cttttaacac tactattata agtggttggt aaaataatat acactaattt
     7561 aaatatagca taaaaaacaa aaaaagtcaa tgaaaaaaat aaaaaaggag aatttaaaaa
     7621 tgatatcacc tattattttg agttctataa atcagaattt aaaggaaata gaaagaaatg
     7681 aactgcttga aactaatata gaatctggag attatggtct agctctttct gaatctgatg
     7741 taaaagatat aataaattcg agagataata ctttaaaggg gtatggaaga atagaattag
     7801 atataaaagt tacaaaacag ttaatagaaa atatatatac atcacaatat acaaatgtgg
     7861 acaattattt agaggctatt aatgatatgc aagaaatttt ttattattta aaaaatgaaa
     7921 ctgatgacaa gatatgtgat gatgagatta tagaaatact gggtgaattt tatgaaaaat
     7981 tttcaggcaa tatggacaat gtaagaggag aagcagacga atttgcaaag aaatttaaat
     8041 ttggagaagt ttaatatatg ataggagaga ttttgtatgt taaataattt aagtaatatt
     8101 aatataaacg aaaataattt aataaagaac caatattcca tatctctact taaggaatgt
     8161 ctaaattgta aagtgataga tgaaagagaa gtatataata tacaacaaga aatatctcta
     8221 atacttatgg atttgattaa aaaatataca aatggtcaaa gcacatcagt aaaaacagaa
     8281 gttgccgaaa aactgttgat ttcaatatgg tatgctattg atgcatatat aaataagctt
     8341 ggagatatag aaaaaagagt tgtggagttg actgctaaga atgttaaaaa agtgtacctt
     8401 ggaggcattg aaatattaga ggaagagtta gttaggctga aagattttta taaactaatg
     8461 atgagtagaa aattggatac taattcaata gcatataatg ataccttgat ggagttatca
     8521 agtttcttta aaagttatat tgtaaaattt gaagctcagg acgttccagt aagtatagat
     8581 tatccattgg cactagatga tatggatgtt aagggtattt attatgtgaa gaattatatt
     8641 gagagtatag atatagaaaa tagattttgt aacctattta agaaaaaaga tagagaaaaa
     8701 ttattttatg actatggggt gatttataaa atagattgtg atgttatact tgtaaattta
     8761 tttgaactta ttataaataa ttgtattttt tctactattt taggaaatga agtgattaat
     8821 ttagaaattt caaaatatga gtatgaattt ttagaaaaac aatttaaaaa tatgtacaat
     8881 aatattactg aagataaagt tataggctct gaagaaagta atataattga attgattgaa
     8941 gtagatgata gttttaacac tgaagtagat agtaatttgg attataatga ttttgaagaa
     9001 attaacagag aaattagaat aaataaaaaa ataactttac tacttatgga agcaattgaa
     9061 gtattaataa aaagattaag catagaagat gaagatgtaa taagatatat taaaaaatat
     9121 gaaaagatat ttattgaatc tgtgctaagc tcaataaaaa gtaacacttt taaaagtatg
     9181 gtaactgtga gtaaaaataa aaatgaacac ttagaagact atattgttga tgatgaaagt
     9241 aaattggatg atgaatcgtt tagagagata ttcaatgaga tattagatag tacatctata
     9301 attgaaaaaa taagaataat aaaggaaaat atcaatgcta aaaaagactt tatcgatatt
     9361 cttgaatctg aatgtttatt tggagaagag tatttaatgt tgtttgcatc attaagtgaa
     9421 ttagaattgg caatattggg tagtgtagta ttttatgaag atattagaat gagagaaatt
     9481 aatatattag aatttatgtt aaatgagaaa actgaaacta ctttatggaa gacagagtac
     9541 attaaattta tgaagaggca aagtgaagat aagatatact ccatagaaga gcatatgaat
     9601 aaaattaatt aacaaaaaat tgtgaataaa tagaatgtta gtatataata taatctgtaa
     9661 agcatatcaa aaatacatat atttggagga aaattatgaa agagtatatc ataaataatg
     9721 ttgaagaaat aagagaagaa ttaattgatt tatctaagaa aatatgggaa aatccagaat
     9781 tagcctttga agaaaagtat gcaagtagca ttcaaaaaga atacctaaag tcaaaaggat
     9841 ttaaaattga agaagtagaa aatttaccta ctggatttat agcaagtttt ggagagggta
     9901 aacctgtgat tggcatactt ggagaatatg atgctttacc agagctatca caatgtgtga
     9961 gtgcagaaag aaagccattg gtagaaggaa aagctggaca tggatgtggg cataacctgt
    10021 tgggtgtagc aggtgtagga gcagttgttt ctataaaaaa attaatagaa gaaaataaat
    10081 ttaatggaac aataaaatat tttggttgtc ctgctgaaga agaaggtgga ggaaagacag
    10141 ttatgtgtat aaatggctgt tttgatgatg tagattgtgc atttacatgg catccttttg
    10201 atataaatgc accttggaga ggtggaagtc tggcaaactt atctgtaaaa tttaaattta
    10261 aagggataac agcccatgca gctcaagctc ctcataatgg tagaagcgca cttgatgctg
    10321 ttgaaattat gaatgttgga gctaattatt tgagagaaca tgttattgac tctataagaa
    10381 tgcactatgt tataactaat ggtggtggaa gacctaatgt agttcctgga tttgctgaaa
    10441 gttggtactt tattagaggt aaaaaagcaa gggatgcaga acatgttctt gatagattaa
    10501 taaaagtagc tcaaggagca gcaatgatga cagaaactga aatggagtat aaggttacag
    10561 atggaatata tgactatata ccaaaccaat gtttgactga tttagtttat gacaatatgg
    10621 tttttgttgg ttgccctaaa aatactcctg aagaagaaga atttgcaaaa aaattgtgtg
    10681 atactcttac tagagaagaa agattaggtg tagcaacatc attacaaaat gataaatcta
    10741 ttgtagaaag ttatatacac aaggaaatag ttgacggtga caaagataaa ggactagctg
    10801 gttctactga tgttggagat gtaagttatg taataccagt ggctcaattt gcaatggcag
    10861 cttggcctgt tggaattgca agtcatacat ggcagtcatg ttcatcagca ggttcaaata
    10921 taggattttc tgctatgata aattcagcta aagtactggc ttgtagtgct tatgatgtat
    10981 ttatgaatac taaaattatt gatgaagcaa aaattgaatt tgataaatca ttagatggac
    11041 aaaaatttaa accattggta taattttggt aataaaatat ttctgggggg aatagctatg
    11101 agagaaaaag ttgaaaaagt gcttgaggaa aagataaaac cagtattaca aagagatggt
    11161 ggagatgtag agcttataga tgtaaatgaa aatggtgtgg ttttagttag actacaaggg
    11221 gcatgtagtg ggtgtccagg agcgactatg acaatcaaag ctattattga aaatgttttg
    11281 gtgagtgaag ttccaggagt aactcaagta ttaggtgttt aatagttagc gtttattaaa
    11341 tgtatgttat acataagtat ttaataaaga agtaaaaaaa atagtgaagc aatatatatg
    11401 aaaaagtatg ataaagataa taaaacatta tagaaataat gaaatatttc atatgtatat
    11461 gaagaaataa gaaaaaatag tgaagcaata tatatatgaa aaagtatgat aaagataata
    11521 aaacatttat agaaataatg gaatatttca tatatacata tgaaaaaata gtaaaacaat
    11581 atatatatga aaaagtgtga gaaaataata aaatactata gaaacaatta aatattctat
    11641 ataggtatta agaaacatga aagaaataat ggagtattat agataaatat gtaaaagtgt
    11701 gaaagaagga tgtagataaa tttgtcttat acaaatttac ctacagtatt tcttacacac
    11761 tttttatttt actatccaac cataccaata aacataccta taaagtatgg aactaaccat
    11821 atagtccata caactatact aaacttatga aagaattttt tcttttcctc atcatctttg
    11881 tataggacaa aagtagccca acttgcatga aaaagcataa gcacaattgc aagaagacct
    11941 gtaatactat gaaggttttg tgataaagca gattttactt caaaagtatg gctgttggct
    12001 atgttactca ttgtaaatgt tcctaaagta tcaaatataa acccaagcca aaatataatt
    12061 acatgtttct tttttaaaat ttttgccttt ctttctccaa atacacctat agtgtagaaa
    12121 attaaagctg atgttataaa tactattgca agtattaatt tataattcat aattatctct
    12181 cctttacttt tgccaatata tttctaagat tttttactaa gtcactcaat tcgttttcat
    12241 cacaattttt aaaaatatca tcaaaacttt cttgcatttt aattttaaaa tttgtggcaa
    12301 attcaaatcc tgttgttgta aacttaacgt atgtatttct tttatcatta gatttcttga
    12361 atttttctat atatccaatc tgttctaatc tgctgattat gcctgaaacc gtacctttag
    12421 ctaaagacat ttcatcacat agttgtgata ttgtaagttc ttgattatga gctaccagtt
    12481 ttataactat aatctgttgg tgtgttaatc cattttcttt aaaattttct tctactgtgt
    12541 acatagtttt agaataaagt tcttttaaga gcatggctat tctaaaacag tcataattac
    12601 ttttcaataa aatcacctct taaatggttt gcatacaaac taatataata tacttttctt
    12661 atatagtcaa cctaaaattt tataatttta tagaaaataa tgaagaatag aacataaaaa
    12721 ttttatttta ggtataaata aaataatttg atgaaatttt aacaattttt aaaaagtttg
    12781 tttacacagt taataaatga tgctaaaatt aactcataga taaatataga aaatttaatt
    12841 ttgtttaatg ataatggaac cacgaaggtt ttaacgttac tttacgtttg cttttatgtg
    12901 gtttttttat tgcaacaaag gggggttggg gtttgttaaa ggatatactg aaaaagttgt
    12961 tgcagtttat tttagtaatg tttttattat cttttattgt tttttacatg gcaagacttg
    13021 ctccaggaga cccattaatc tcttactatg gggatggagt agaaagaatg agtacacaag
    13081 aaaaagaaaa tgctatgaaa aaacttggtt tgaatgaacc aatatacagt caatatataa
    13141 aatggatttc aaatgcatca aaaggggaat ttggaatttc atttaaatat aaacaaaatg
    13201 ttacttctgt tataaatgat gtctatataa acactataat attaggtggt tcagggtata
    13261 tacttacctt tgtgctagcc ttattgttag gaatattttg tacattacat gaagataagc
    13321 tcattgatag aatcatatgc aaattaggaa caataaccaa ctgtattcca tcattttggg
    13381 ttgccttagt actcatactt atatttagta taaacttaag tatacttcca agtagtggtg
    13441 cttattcaat gggagaagag tccagtatat taagtaggat atcacatcta atcttacctc
    13501 ttacagtact tatattaagc catctatggt attacacata tatgataaga aataaattac
    13561 tagaggaaat aagagaagat tatgtgcttt tatgtaaagc aaaaggttta aataatagaa
    13621 ctattgtttt taagcattgc ctgagaaata taatgccatc atatattagt ataatggcca
    13681 tttcgatacc tcatatacta ggaggaactt atgtagttga aaaagttttt tcttatcctg
    13741 ggttaggaac actttgtttt gaaagtgcaa agtatcatga ttataatatg ttattagttt
    13801 tatgcttaat aacaggggct ttagtagtat ttgggaatat gttagctcaa attataaaca
    13861 ataaaataga ccctagaatg aaatatgata gaggtgatac taatgaagcc acaatatagt
    13921 gattttgaaa ttgtaggaga aaattatatt ttagtagcag aagatgaaca tatagaaaca
    13981 aagaaaacct tatctaaaaa aattaaagaa ttaccatata tatcaattat tattttatca
    14041 atcatagtca ttggaagtgt attctcatct tttataatga cacatgaacc aacatacatg
    14101 gatttagcaa gttctaattt agctccaaat aaaacttttt tatttggaac agactcaatg
    14161 ggtagagata tatattctat gatatggtat ggaggaaaaa tatctttatt tataggacta
    14221 ttttccactg taatatcaac tgttatagga attgtttatg gtagtataag tggttcagca
    14281 tctgaactcg tagatgatgc aatgatgagg tttacagaaa tcatacttag tataccttca
    14341 atattaatta taatatttgt acaagctata cttggaaatt caaatccagt atctatgtcc
    14401 atagtaatag ggataacaag ttggatgaat atatctaaga tagtgagaac agaagttaga
    14461 caaataagaa atagcgaata catactagca gctaagagca tgggaggagg attcttctat
    14521 gtattaaagc aacatttact tccaaatttt gtggcatcaa taatgttcat ggttgtaaca
    14581 aacataggcg cagctattgg tactgaatcg acacttagct ttttaggaat tggtttacca
    14641 atagaaattg tttcttgggg aagtatgtta tctttgtcag aggaagcatt actttcaaat
    14701 agatggtgga ttatactgat tccgggtata tttttggtta ccacattggt gtgtataaca
    14761 aatataggta attatataag aaagaataat aataaaaaat ctagtaattt ataaatcagc
    14821 atttgagtac taaataagca aatactgagg tatttgaaat atctatttaa ataaatgggg
    14881 tcaaagcttt gaaaaatatt ttataaaaag caaaaggggg agcatcatga aattaaagaa
    14941 gttaaaagtt ttaagtttag taatgatact tagtctaatg gcaggttgtt ctagtggagg
    15001 agacaaagat aagaaggcag atacgcctaa agatggaaag gtacttgttt atggaagtaa
    15061 tgactataca agtataaatc ctgcactata tgaacatgga gaaataaatt cattaatatt
    15121 taatggatta acagctcatg atgaaaataa taaggtggtt ccttgtctag caaaggactg
    15181 gaaatttgat gaggcaacaa atacatatac ttttaattta agagatgatg ttaagtggca
    15241 tgatggagag aaatttacgg caaatgatgt taagtttaca atagaaacta taatgaatcc
    15301 agataatgct tctgagatag catcaaatta tgaagatata acaaaaattg atgtggttaa
    15361 tgataatact ataaaaatta ctttaaaagc tccaaataca gcaatgcttg attatttaac
    15421 agtaggagta ttaccaaaac atgttttgga aggtaaggat atagctactg atgaatttaa
    15481 tcaaaaacca ataggtactg gaccatttaa acttgaaaaa tgggacaaag gtcaaagtat
    15541 aacacttgtt aagaatagtg attattttgt aaaagaacca ggattagata aagtagtctt
    15601 taagattgtt ccagatgata aggctaaggc aatgcaatta aaatcaggag aattagactt
    15661 agctcaaata actcctaaag atatgtctaa ctttgaaaaa gacgaaaaga attttaaagt
    15721 aaatataatg aaaacagccg actatagagg tatattatac aattttaatt ctaaattctt
    15781 taaagataaa aaagctaaag gattaccaaa tgctttaagc tatgcaatag atagaaaagc
    15841 aatagttgac agtgtattat taggacatgg tgtaccagca tattcacctt tacaaatggg
    15901 accatataat aatcctgata tagaaaagtt tgaatataat ccagaaaaag ctaaacaaga
    15961 aattgaaaag ctaggttgga aattaggttc tgatggaatc tatgaaaaag aaggtactaa
    16021 gttagctttc gagataacag caggtgagag tgaccaagtt agagttgata tggctaaaat
    16081 atgtgcacaa cagttaaaag aaattggtgt tgatgctaaa gctgttgttg taactgaaac
    16141 agattgggct aatcaagatg ctcatttaat aggttgggga agtccatttg acccagatga
    16201 ccatacatat aaagtatttg gaacagataa aggtgcaaat tacagtgctt actctaatcc
    16261 tactatagat aaaatacttc aaaaagcaag agaaacagaa gataaagatg aaaaattaaa
    16321 gttatataaa caattccaag tagaaatgac taaggatatg ccttatacat ttattgctta
    16381 tatagatgca atatatgttg gaaaaccaaa cataaaagga ttaacacctg atacagtttt
    16441 aggtcatcat ggtgtaggta tattctggaa catagctgac tggacaattg aataatactg
    16501 aaattaatta gaaaccttag ggataaaaat atatccctaa ggtttgaata gcataggggt
    16561 tgatggtttt tggaacattt attggaggtt aataatctat ctgtaagttt taaagtagaa
    16621 gaaggagaag ttcaggctgt aagaaatgta tcttttaatc taaaaaaagg tgagacattg
    16681 gctatagttg gagaatctgg ttgtgggaaa tctgtactgt gtaagagttt gatgaggata
    16741 ctcccataca atggatatat taaaaatgga gaggttttac ttaaatcaag tgatttggtt
    16801 aagaagtctg aaaaagagat ggaagatata agaggaaaga atatatcaat gatatttcaa
    16861 gaccctatga catctctcaa tccaactata tcaataggaa agcaaattgc agaagctgta
    16921 atcattcatc aaggtataag taaaagtgaa gcaaaaaaga gagccataga gcttattgaa
    16981 ttggttggaa ttgataatcc tgagaaaaga tttaaacaat ttccacatca tttttcaggt
    17041 ggaatgagac aacgtatagt aatagctata gcacttgcct gtaatccaga tgtattgatt
    17101 gctgatgaac ctacaactgc tttagatgta actatacaag ctcaaataat tgatttaata
    17161 aaggatttac aacataaaat aggtttgtca ataatattta taactcatga tttaggagta
    17221 gttgcaacta tagctgatag aatagcagtt atgtatgcag gaaagattgt agaaatagga
    17281 actgtagagg atatatttta tgaccctaga catccatata cttgggggtt gttgggttca
    17341 ttacctactt tagattcaca ggatgaatat ttatataata tacctggaat gcctccaaat
    17401 ttattgaacc caccaaaagg agatgctttt gcaataagaa ataaaaatgc acttaagata
    17461 gattatgaaa aagagccacc aatgtttaaa attaatgata cacatagtgc agcaacttgg
    17521 ttattgcacc cagatgcacc tgaagtagat gtaccagtta gagtaaattg tggaagggtg
    17581 atttctaatg aatagagaaa aaatactgga aataaaaaac ttaaagcaat attttcattt
    17641 ggacaaaagt actactgtaa aagcagttga tgatataagt tttgatatat ataagggtga
    17701 gatatttgga ttggtaggag agtcgggttc aggaaaatct acaactggta aaactataat
    17761 aagactacat gaatctactg gtggagaagt aatttacaaa ggaaattgca tatcagataa
    17821 aaaaacgtac aaatttataa aaaaagatgt aaacaaaagt atgcagatta tttttcaaga
    17881 ttctacttca tcattaaatc cacgtatgac aattgcagat ataatttcag agccactaaa
    17941 aattcaaggt atctgtaaaa ataaaacaga tagaatgaat aaagtatatg agatgctaaa
    18001 gctagtagga cttgatagaa gttatgcaaa taagtatcca tctgattttt caggtggaca
    18061 aagacagcgt attggcatag cacgagctct ttcagtagac ccagaattta taatagctga
    18121 tgagcctata gcttctttgg atgtgtctat acaagcacaa atagtaaatt tatttaaaaa
    18181 attgcaacaa gaaaagaatt taacttgttt atttatagct catgatttat ctatggtgag
    18241 acatattagt gaccgtatag gagttatgta taatggaaaa ttagtagaat tggcagattc
    18301 aaatgaattg tataataatc ctattcatcc atatacaaaa tcactacttt ctgcaattcc
    18361 tgttcctgac cctagatatg ctaagtcaag aaatagaata gagtataatt ctaatggata
    18421 tgattgttca aatgaaaagt cattaagttg gattgaggtt tcagatggac attttgtata
    18481 ttcttctaaa tcagaaataa ataaatatca acaaaattta aaagttgttt agtataaaaa
    18541 tacttgaaca ggtatttatt tgaaatacaa actaaaaccc tatttttgga gaaattttaa
    18601 aactactcca aaatagggtt ttttatttct acttttcaaa tataataaat tcaaatataa
    18661 taaaattaaa aacaagtaaa gtatgttgtt aagaaagtgc tataaaatta aaaatagtaa
    18721 tgtagaaaaa atgagcaata atcttatttt ttctacaata attttctata atttaaagta
    18781 aggaaagttt gataaattat aaccttagtt aaagaataag agtttattaa aaaataaagt
    18841 gtgtataggg gttttagtta ataaaatatt ataatgtagg ggagcttgta atgatatata
    18901 aaaaagataa agtaatgaaa gttttagaat catcatatag ggaaaatgaa aaagggatta
    18961 cagctaacca attatcagaa aagacaggaa ttgcaagaaa caatataagt acatatttaa
    19021 accaattatt caatgagggt atggttacga agataaaagg aagacctgtg tattatatcc
    19081 ctgcaaatct taaccttaac aagaatgaat tgcttgatac acaaagtgag ttgcttggca
    19141 taaaaaaagg aacaaaagta gaaaacaaaa taaaaaaaga tagttttgaa acactaatag
    19201 gtaaagaaca tagtttaaga cctataatag aaaaatgcaa ggtcgcaatg ctctatccta
    19261 caaatggact tcatactatt atctatggag aaacaggagt aggaaagagt atgattgcta
    19321 gatatatgta cgattattct atagatagtg gaattagaaa aaataaagct ccttttgtaa
    19381 catttaattg tgctgattat gctaataatg cacaattact tatgggacat atttttggag
    19441 tagaacaagg agcttataca ggtgcagaat cttccagaaa aggtcttttg gaaattgcaa
    19501 atggagggat actattttta gatgaaattc atagactacc agcagaaggt caagaaatgt
    19561 tgtttacttt tatagataag ggaacattta aaagactagg agatgcaacg aaagaaagag
    19621 attctaaggt attaataata tgtgctacta cagaaaatcc gacatcaaca ctattagata
    19681 cattcaatag aagaatacca atgaaaatag aaataccatc actgagagat aggacaatga
    19741 ttgaaagaat ggaattagtc aaaaattttt tcaaggaaga atcaaaaagt atttctattt
    19801 caataaaagt acataaagaa ataattaaat cactattact ttatgattgt aaaaataatg
    19861 taggtcaact tcaaaatgat ataaaattag ctgttgcaaa tggataccta agatataaaa
    19921 gagataataa agaaccagtt tatatagaga aaaaatattt tgataaatct ataaatgaag
    19981 tgagtgatga ttatagagag aagtctatta aagcagatga agttataaca catggaatag
    20041 aatattttgt atttacgtca ttaggagaag aagaagttat taactacaat gatataaata
    20101 ttattaatgc agtaaatact aatcttaata aatctgtaga aatgaatata gcagatgctt
    20161 ctctatttaa taatgttaag tttaaaagca tgtgtgaatc aataagacaa attattaaag
    20221 aagatgaaga aatagatata agtgaatcaa tatttaagtc aatggcaatt tatataaaaa
    20281 gcgtattgga taaaagtagc tctacaaaca acatagattt gaatcaaatt agaagaaata
    20341 acagaagtga atttaaaaca gcactaaaaa tagttggaat aattgaaaat gaatttgatt
    20401 tatttttatc tatagaaact gtagcatata ttacattatt catagtacaa gctaaacaag
    20461 atgaacaaaa taaaatagtg aataacatta atattatagt tgctatgcat ggagaaacta
    20521 cagcaagtaa tggtaaaagc agttggtgat attattggaa agtgcagtgc tatttctttc
    20581 gatatgaagt tagataaaag ctatgatgaa gttattgtag attttaaaaa tttaattaat
    20641 aatatagaca ataaagatgt attactattt acagatatgg gttcattaaa ctcctttgat
    20701 gaaattataa agaaagaaaa aaagtgtgaa gtaagagtaa ttcctatggt gacgacatta
    20761 actgtgttag aagcagttca gaaggccaat atgggattac ctttaaatga cgtatacaac
    20821 tctatcacta acacgagaaa gtattatttt ggaactaatg aaattcaaaa taaagaaaat
    20881 ctgtctaaaa caattattat agcatcacat gtttcagaag gtgtagataa taaaacaaga
    20941 aaaatattag aagaaaagat gagtaggtac ttagatggaa tagatattat atctgtccca
    21001 tataaaacag aaaaagattt aagtttaaat attacaaaac ttaaagaaag tagtaatatt
    21061 gttgcagtaa tcaatgaaca aagaattaat attagaggaa ttgactatat tagcaaaaaa
    21121 gatatagata aggatgaaaa tatcaataaa ttaaaaaata ttataaagat aagtataggt
    21181 tatgatgatg tagttgaagg tttaaagaca tccttaaaaa gctctaatta taataggata
    21241 tttaaagata taaaatatgt gtctgatgaa ttgtttttag tatttaatat agaaaagaaa
    21301 tacgataaag taattggact tatgatgcat ttagctttca tggtagatgg gttgattgga
    21361 aatactagag aaattgaaaa gcttgataaa gaaaaaacac ttgattatca caagagccta
    21421 agcaaaataa aagatatagt atctcaatta gataaaaaat ataatataga aattaatgaa
    21481 aaagagtgct atcaaatatt attaatatta gaatatgcag aaataatcga aaaagattac
    21541 caatgagtat aaaaacttat ttggtaattt tttttagtct aaaataatat taatattaga
    21601 cacatataaa tatacttaga caaagtatct aagttttgat gataaaaatg attgaaataa
    21661 cagtgtttta aagtgtctaa aaaaactggc ataatacttg cttatattat taaatgagta
    21721 atataaataa ttaaaattta aataaaaagg agtaaataat atgcaagata aaatattaag
    21781 agataaaaag gcaaatgacc catgggcaag aatgactaca agaaacggat ttagtgcaga
    21841 cgaaataata tcgtcatttc aaaaagctat aagaagaaat atggtggaag aagcttgtga
    21901 atttgcttat gaactatata tatcatcacc agaattagaa gataagctat ggagaaggat
    21961 acttacaata tcagttgaag atataggaat gggggaccca tcagctgtaa taatcataaa
    22021 taatttaaat caagtaagaa aggaatattc atatgcagat ggagacagac ctttattttt
    22081 catacatgca ataagatatc tttgtgcatg tgagaaagat agatcaagtg atttattaaa
    22141 aaatataatt ataaaaggat ttgcaatggg taaagttcca gaagttatgg acgtggcact
    22201 tgacaagcat acatatagag gattagaaat gggaagggat tcattccact ttttaaatga
    22261 agctagtata gtaataccag aaaaagaagt agataatgat tataaagaaa gatacttaaa
    22321 aatattagaa aaatataagc aagaagaagt tatagatacg gctttcaagt ttaatcattg
    22381 gcagtactaa gaattagagt aaatattaat aagaattaaa ataaaataat aagaattaaa
    22441 gtaaaaactg ataggaatta gagtaagaat tggctgataa aaaattagaa aataaaagag
    22501 gttagatatt gtaggagggt aattatgaat attttattag tatgtgcaaa tggagcttca
    22561 acaggtgtac ttgtggaaaa gatgaaaagt ttttgtagtg agcatgaaaa gttaaaaact
    22621 aaaactataa atatagaggc aacatcattc gaaaatttaa aatcatacat tgaagctaat
    22681 gatacagatg tagtacttgt agcaccacaa attaggttca aggaagatga tgtagttgaa
    22741 gcttgtaaga attacaaaat agcagttggt ttaatagata ctaagcatta tggtcgtatg
    22801 gatgctcctt ctgttatgaa gtcggcaatc gaattatata aaaatagata attgggggga
    22861 ataaaaatgg aagcacttca aaataattta agaagatttc ttctaccaat agcacaaaag
    22921 attgaaaaac aaaggcatct tcaagctata aaagaaggta tgataagcat taccccaata
    22981 ataattgttg gttctttaag tatgttattt atggcattaa acaatatgtt acctgaagga
    23041 agtgcaaaaa cattattatc tgaaaatatg gatacattac ttatccctaa taaatttact
    23101 atgagtttat tatctatata ttcagcattt tttatagctc aggcactagc taaaaaatat
    23161 aatcttaatc atgtagaaat aggtatgaca gcagttgttg cacaacttgt tgtatgtgga
    23221 caagtagtag atggtgtatt agatacaagt tatttagatg cacaaggttt atttgtaagt
    23281 atattagtag cacttttagt agttgatatt actaagttta tgaatgataa aaatctcgta
    23341 attagatttc caaaagaagt acctagtgta gtaaataaaa gttttagaaa tttaactcca
    23401 atgatagtat gtataatgtt atttacagca atagcagcaa taactaaaaa tgtctcaggt
    23461 caaccattac cagctattat aatgaatttc ttagcaccag ctataagcag tgtagataat
    23521 gtttttgcag taactataat actatttata actcaattat tatggttctt tggccttcat
    23581 ggagcagcaa taacttcaag tatatggatg ccaatagcag ctacatatat ggcagaaaat
    23641 gcaacactga tagcagcagg aggagaccca aaatatgtgt ttacaatagg attttactat
    23701 ggtttcttgc aagtaactgg ttcagggata actttgggat tagtgtacct tatgtcaaga
    23761 agtaagtgta agagttttaa tagtatggga aaagttgtga ttttaccatc actatttgga
    23821 ataaatgagc cagtaatatt tggaacacca atagttatga acccatatat gtttgtacca
    23881 tttgtatttg gacctgtatt ggttggagca cttaatttta tggcattaaa agtaggatta
    23941 gtaggattac ctattgcaga accaccaggc tttttacctc caggggttgc agcatttttg
    24001 atgacattag attggaaggc aatcgtatta gtatttgcaa gtataatact tatgaccttg
    24061 atatattatc cattctttaa gataatggag aaggaagaat taaataaaaa tgcagaatta
    24121 gctacaacat tagatgatga tagctttgat ttttaacaag tcattagaag gagaaggata
    24181 tggtacctga aatggaagaa aaagtattta gtataatatc agaagcagga gatgctaaaa
    24241 gtgatgtaat gatgtctctt aaggaaataa aaaaaggtga ctacaataaa gcaaaacacc
    24301 ttttaaattc agcctcagag aaaatacaaa cagcatcaaa gcatcattta gaattactat
    24361 caaatgctat gaatagtgaa gatagtggga ctgatttttt agttgtgcac tctgaagacc
    24421 attattcaaa tgcattattt gctcattctt tagtatctga acttgtagat atatttgaga
    24481 tgatggaccc aagaattaat aagaattaaa tttcttgtaa atagtgcttg aagtttggct
    24541 atagtttatt aaatcaatag atttaataaa tgaactccct tttactttaa agtactagta
    24601 tttataaatt tatttataaa tacagtaaat ttttaaatag aaaaggagat attattatgg
    24661 aaaaattttc agtagtaata gctggaggag gaagtacata taccccagaa atcatattaa
    24721 tgttgctaga taacttagat aggttaccat taagagcaat caaattatat gataatgatg
    24781 aagaaagaca aaataaggta gctaaagctt gtgaaatact aataaaggaa aaggacccta
    24841 atatagagta tttagcaaca acatgcccta aagaggctta tacagatgta gatttttgtt
    24901 tagctcatat aagagttgga aaacttgaaa tgagagaact agatgaaaag atacctctaa
    24961 aacatggagt agttggtcaa gaaacttgtg gaccaggtgg aatagcttat ggaatgaggt
    25021 ctatagaagg tgttcttgaa aacatagatt atatggaaaa gtattctcca aattgttgga
    25081 tgttaaatta ttctaatcca gcatcaataa tagcggaagc tgttagaagg ttgagaccta
    25141 attctagggt aataaatatt tgtgatatgc caataggtat ggaacataat atagcaagga
    25201 ttgcaggact caaatctcgt aaacatatgg atataagata ctttggatta aatcactttg
    25261 gttggtatac ttcaattaaa gataaaaaag gaaatgaact tttaccaaaa ttagtagaac
    25321 atataaaaga atatggtttt ataaatggtg aagaaggaat gaaaaatgac aagaaagata
    25381 gttggtttga aactaattta tttactaaag aaatagtaaa aacagattta actacaatac
    25441 caagtagtta cttaaagtac tatctatttc cagattatgt tgtaaatcat tctgatgtaa
    25501 attatactcg tgccaatgaa gttattgatg gaagagaaaa agaagttttt ggttcatgtg
    25561 cattgataga aaagcaagga cattctaagg gcagtgggct taagatagga atacatgcag
    25621 agtttatagt ggatttagct acagctctag cttttaatac acaggagcgt atgttattga
    25681 tagttcaaaa taatggagct atagaaaata tacaagatga tgctatggtt gaaattccat
    25741 gtatagttgg aaaagacggg tatgaaccat tatcaatagg gaaaatacca acattccaaa
    25801 aaggtttaat agaacaacaa ttagcagttg aaaaattagt agttgatgct tggattgaga
    25861 agtcttataa taaattgtgg caagcactta tgctatctaa gacagttcct agtgcaacag
    25921 ttgcaaaaga attactagat gatttaatag aagcaaataa aaattattgg ccaaaactta
    25981 attaagaata atatattttt aaagttccta cagtttagta catagcctag ttaatgtatt
    26041 tgtagggact ttatttattt acaaatgaga tacaagtggc aaacatagtg tattttgcag
    26101 ttaggaaagt aggtttagtt acttgttagt tggattaaat ttaggtacaa taatttacta
    26161 tcaatcaata gaaataacca gagaagttgc ataataaaaa atttacactt ttaacaggat
    26221 ttatgtaggt gtttatagaa ataaataaat atgtacataa atacataatt atttttaagt
    26281 ctaaaataca agaatggaga gatggctatg aagtggagag attatgctga tgatgtaaat
    26341 ttatttgagg attttgataa aactatcaag cctctaactg acgaacaaag aagaaaaaat
    26401 ataaacactt taattgcata tttttcaaaa gaagttcctt ctaaaatata caatttatca
    26461 aatgatgaaa ttaagcctag agatatactt agaggacttt tgaatgtata tccaccaaaa
    26521 gaaattgctc cagagatact taatatgtta cataatttat tgttgattga atgtgaagaa
    26581 agagagttag tagatgtaaa tgatatagaa gaagtagagg aagggattgc catatggaga
    26641 ggtaatataa cgaatttaag agcagatgca atagtaaatg ctgcaaataa caagttatta
    26701 gggtgtttac aaccattaca tctatgcgtg gataatgaga tacattcatg cgcaggtcct
    26761 agacttagag aagattgtga caaaataata aaaaaacaag ggcatttaga atatactgga
    26821 gatgctaaga taactagagg ctattgttta cctgcaaaat ttgtagtaca tactgttgga
    26881 cctattgtat ctggtggtca gccaagtaaa gagcaagaaa agcaactgct tcattgttat
    26941 aaatcatgtt taaatacaat aaaagaaatt gatgaaataa aaaatattgt tttttgtgga
    27001 atttctacag gtgtatttgg ttatccaaaa aaagaggcag ctaatcttgc tgtaagtaga
    27061 gttagactat ggctaaaaga aaatcctgaa aaaaatttaa aagttgtatt taatgtgttt
    27121 actgaagaag aagaagagaa atatagaaga atatttaaat aatattatgt aacagaatcc
    27181 taaaaatact tattatataa tacaaattca ttgtataaga taataattta caagtagtaa
    27241 aaattaataa aatataattt gtatttttaa taaataagta ggtataatag aaattataag
    27301 aatagtataa tcaattatgc tattacttag aaaatataat ataggaaaat tgtatggtaa
    27361 ataaatatga aaaatagttt taaaaattgt attagtttaa gaaataaatt tataattata
    27421 tcaattgtgt ttctattaat cctattaatt gtatcttata tatttggcaa attagtgtat
    27481 aatagttcgg ttggtttaga gcagttaata aaaaacgaag atatagtaaa aatattttct
    27541 gagagaaaag ataaaccact ggagaaatta aaaaactaca agacatctga attaatgata
    27601 caaagttcta ataattacaa gcttgaaagt ctttttataa cttcaaatat aaagacaaga
    27661 gatactatga tacttgttca tggaattgga agtagttact atgaaatgtt aaaggtagca
    27721 tatagatatt tagataaagg ttataatgta ttagtttata atcaaagaaa tactggtaat
    27781 agtggtggag ataattacac atttggtctt tatgaaaggt atgatttaga tagtctagtt
    27841 aaatttgtaa aaaataaatt tcctgaagga agattaggag tgcatggatt ttccatggga
    27901 gcagggacag ctgctatgca ttcagagata aattcaaaag atgataaggt cgatttttat
    27961 atactggatt ctccttattc tgaaatgaaa gatgcaatta gaatgggagt actagaaaaa
    28021 cgaataccag atatattaat caattatgtg gtgatttgtg gagatttata taataagttt
    28081 aaatctggat tttggtacag tgatgtaaaa ccatatgagt cagttgagaa atctaatgta
    28141 cccatactgt ttatacatgg aacaaaagat actgtttgta attatcaaaa tagcaagaaa
    28201 atgtatgatt tagttaaaca tgataaaaaa gacctatggc ttatagaagg tattggtcat
    28261 gttgatggat ttgaacatga tagtacagta tacttcaata aaatatttaa atttatagat
    28321 agtaatgttt taagtgataa aagttaattg aatattagta gcttatttat ttttgtattt
    28381 attaaaaaat agataggcta tttttatgtt taaatataag ttttgtttag taatgtgaat
    28441 taatttctag taaaaatgaa aataactacc aattaaattc atttatacct caaataaaag
    28501 agttagtatt agattacaat caaatctaaa gaaaagagag gtagtatata tgaaaaacaa
    28561 aaataataaa gataaaaaca tgttgaatat taaagtattg agagttctag gagtgctatt
    28621 aatacttttt gattatacgg gtgatacttt gaatttttat aatttcttcg caaagccatt
    28681 agtatcgggt aatttatata agacaacagg tactgactgt attgtatttt tattatttat
    28741 tgtagagtgt gtatacctag tgggaagata tatattaaaa gttaagataa attggagtga
    28801 gattattgaa aatgagaaag gtaattttat aaataacttg ttggttgaaa aaatacaaga
    28861 agaaagcaac attgtatata atttattaaa ctcatatgtg ttgccacata tatataagaa
    28921 agcaagggtg ttgatgttta aaacatatca tatgttaatc tacattaaag aaaaatacta
    28981 ttatgtaaat atatattaat ataatatgat tgcacaa
//
LOCUS       DAELSY010000032        28998 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.032, whole genome shotgun sequence.
ACCESSION   DAELSY010000032 DAELSY010000000
VERSION     DAELSY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28998
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.032"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(93..521)
                     /gene="ytfJ"
                     /locus_tag="KPG09_001324"
     CDS             complement(93..521)
                     /gene="ytfJ"
                     /locus_tag="KPG09_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBG0965945.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            complement(475..1104)
                     /locus_tag="KPG09_001325"
     CDS             complement(475..1104)
                     /locus_tag="KPG09_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBG0965946.1"
                     /translation="MELNELFNFKYLIIAVIFIISVTMLILISHINILIIADINNKDI
                     CLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKI
                     KIHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGN
                     IRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTKSYGNNS"
     gene            complement(1266..1805)
                     /gene="scpB"
                     /locus_tag="KPG09_001326"
     CDS             complement(1266..1805)
                     /gene="scpB"
                     /locus_tag="KPG09_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBG0965947.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            complement(1807..2526)
                     /locus_tag="KPG09_001327"
     CDS             complement(1807..2526)
                     /locus_tag="KPG09_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBG0965948.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            complement(2556..3152)
                     /locus_tag="KPG09_001328"
     CDS             complement(2556..3152)
                     /locus_tag="KPG09_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBG0965949.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLIYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGIYQIILSPIYTVLLKIVYLFV"
     gene            3450..4286
                     /locus_tag="KPG09_001329"
     CDS             3450..4286
                     /locus_tag="KPG09_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0965950.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            4307..4729
                     /locus_tag="KPG09_001330"
     CDS             4307..4729
                     /locus_tag="KPG09_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBG0965951.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(4940..6103)
                     /locus_tag="KPG09_001331"
     CDS             complement(4940..6103)
                     /locus_tag="KPG09_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG0965952.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(6157..6357)
                     /locus_tag="KPG09_001332"
     CDS             complement(6157..6357)
                     /locus_tag="KPG09_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBG0965953.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            complement(6563..8239)
                     /locus_tag="KPG09_001333"
     CDS             complement(6563..8239)
                     /locus_tag="KPG09_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBG0965954.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            8456..9193
                     /locus_tag="KPG09_001334"
     CDS             8456..9193
                     /locus_tag="KPG09_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG0965955.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            complement(9310..9768)
                     /locus_tag="KPG09_001335"
     CDS             complement(9310..9768)
                     /locus_tag="KPG09_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG0965956.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(10050..10337)
                     /locus_tag="KPG09_001336"
     CDS             complement(10050..10337)
                     /locus_tag="KPG09_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBG0965957.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            complement(10415..10840)
                     /gene="ruvX"
                     /locus_tag="KPG09_001337"
     CDS             complement(10415..10840)
                     /gene="ruvX"
                     /locus_tag="KPG09_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBG0965958.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            complement(11046..13217)
                     /locus_tag="KPG09_001338"
     CDS             complement(11046..13217)
                     /locus_tag="KPG09_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965959.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYGTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNVGYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIININMIIFEKNN"
     gene            complement(13227..13484)
                     /locus_tag="KPG09_001339"
     CDS             complement(13227..13484)
                     /locus_tag="KPG09_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBG0965960.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            complement(13509..16148)
                     /gene="alaS"
                     /locus_tag="KPG09_001340"
     CDS             complement(13509..16148)
                     /gene="alaS"
                     /locus_tag="KPG09_001340"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBG0965961.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     misc_binding    complement(16216..16454)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(16597..17676)
                     /gene="mnmA"
                     /locus_tag="KPG09_001341"
     CDS             complement(16597..17676)
                     /gene="mnmA"
                     /locus_tag="KPG09_001341"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBG0965962.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     gene            complement(17965..18405)
                     /gene="nifU"
                     /locus_tag="KPG09_001342"
     CDS             complement(17965..18405)
                     /gene="nifU"
                     /locus_tag="KPG09_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBG0965963.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            complement(18459..19652)
                     /gene="nifS"
                     /locus_tag="KPG09_001343"
     CDS             complement(18459..19652)
                     /gene="nifS"
                     /locus_tag="KPG09_001343"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBG0965964.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGRVDLRQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            complement(19670..20116)
                     /locus_tag="KPG09_001344"
     CDS             complement(19670..20116)
                     /locus_tag="KPG09_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG0965965.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            complement(20225..20995)
                     /locus_tag="KPG09_001345"
     CDS             complement(20225..20995)
                     /locus_tag="KPG09_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0965966.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDTLAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            complement(21026..22291)
                     /locus_tag="KPG09_001346"
     CDS             complement(21026..22291)
                     /locus_tag="KPG09_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBG0965967.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            complement(22398..23183)
                     /gene="codY"
                     /locus_tag="KPG09_001347"
     CDS             complement(22398..23183)
                     /gene="codY"
                     /locus_tag="KPG09_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBG0965968.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            complement(23348..25435)
                     /gene="topA"
                     /locus_tag="KPG09_001348"
     CDS             complement(23348..25435)
                     /gene="topA"
                     /locus_tag="KPG09_001348"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBG0965969.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            complement(25468..26568)
                     /gene="dprA"
                     /locus_tag="KPG09_001349"
     CDS             complement(25468..26568)
                     /gene="dprA"
                     /locus_tag="KPG09_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBG0965970.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGYAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            complement(26587..28188)
                     /locus_tag="KPG09_001350"
     CDS             complement(26587..28188)
                     /locus_tag="KPG09_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBG0965971.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLLVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVSIMSFLDDKSRKENGELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISIEEMIEVSKV
                     YSILGMINESKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            complement(28219..28563)
                     /locus_tag="KPG09_001351"
     CDS             complement(28219..28563)
                     /locus_tag="KPG09_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBG0965972.1"
                     /translation="MNNKEKGDFGEEIAVNYLLSKGAKILEKNYRLRIGEVDIIANIG
                     DEVVFVEVKSRSNIKYGYPCESVDFKKRKKIIGVAKYYIIKNNLNNTPIRFDIIEVYL
                     LEKRINHIMNAF"
BASE COUNT         9009 a         5208 c         3006 g        11775 t
ORIGIN      
        1 taaatgttta aagtacacaa caaaatagtg ggatttatac taaccccact actttactta
       61 aaactatata catatttaaa aatattttat tcttacatat tgtttttatc ttgattctta
      121 ttactttgtg atatattttg aattttctcc aatactctag gagttataga taatatatta
      181 tccactagat tgatattact gtctaaactc attattctag tctctccact ttcaactact
      241 acaaatgcta ctggttgtac agatatagcc cctgcgctac ctccagcaaa attcgtatct
      301 gatttttcat tttttaattc aatttcccta tctttatcat catatccttt tgagtattct
      361 cctcctccta taccaaagcc aattgtaacc tttgatatag gtacaaccac tgtagtatct
      421 gtttttatag ggtctcctat aatagtattt gcatctatag aacctttaat tgtttcaaga
      481 gttgtttcca taagactttg tatcgaacct gttgctctca ttactatcac cttccttatt
      541 tcctttattt ttattcataa ttttattaat cattattata atattaaata aagcttttat
      601 tcttggcctt atatgtatcc ttatatttcc taaaacataa tcttttgtaa aatctggatt
      661 tacatttaaa tacatttttt ctgcatcaat catattaaat aaatttccat acaaagtatt
      721 tatcaataca tatacagaag atgttaagcc aatatttcca gttccaaaat taatatttga
      781 ataaagttca tgtattttta tcttttttaa taatctataa atgctcaaaa ggtcatcagc
      841 taataatata cttgaatcta tattattctt catcccctct tttttatcat tatttttact
      901 attttcagct ggataaagtt gtctatttat atttattaaa ttaaacatgt attttatatt
      961 aagctttaaa caaatatctt tgttgtttat atcagctatt attaagatgt ttatatgaga
     1021 tattaaaatc aacatagtta cacttataat aaaaattaca gctattatta aatacttaaa
     1081 attaaataat tcattaagct ccatgtttat attatcatca aaaaattact atttatactt
     1141 aattatccat atttatttta agagaattat aattttatat ttatacaaaa aaacacaaca
     1201 acttagagca ttttaaatct tttaaaattt aaaattacat actaaattat tgtgatttta
     1261 tatttttatt catttgttgc cacttcttga taattttcta taggcggcaa ttcttcaaga
     1321 ctttctatat tcaacaactt taaaaattca tctgttgttt tataaattat tggtttccct
     1381 atcttattca atctaccagc ttctcttata agttcatttt caaacaaagt ttgtaatacc
     1441 ttatcacatt ttactcctct tatatcctct atttctacct ttgttattgg ttgtttgtag
     1501 gcaattatag taagagtttc taatgtagct tggctcaaac ttttcttttt ctttggttca
     1561 ataatcttct ttatatattc tgcatagtct ttatttgtac acatttggta cttattctct
     1621 agcttgatta tttgaatacc tctattttgt tctctgtact cctcaataag taaatttaac
     1681 ataatctcta tttcttttga tgaaagttct ttatttatta tatagtttaa ttcttttata
     1741 cttattggtt ccccatatgc aaacataaca gattctataa tatactttat atcttctctt
     1801 ttcattttaa cactccgaac tttttcttat tagaatatca tcaaaaaata tgtcttgtac
     1861 tactactatt tctttttctt ttataagctc taatatagat aaaaaagtag ctattatttc
     1921 atcattttca tatgatttta taatatttgt aaaagtaact tcaatcttat cttttataat
     1981 atctcttata tacaatatct tttcttccac agatattatc ttcttcctta ctattttgtc
     2041 tagtttctca tcattagcat tttctatctg tgatgtttta actttaaata tgtatggaag
     2101 tatatttttt attgcttcaa ttgatatatc ttctaaatcc acattgtcat caataattat
     2161 ctcttcttta tttctataaa atctctcatt aacataagtt atattttctt ttatatcttg
     2221 tgatgctacc ttaaactttc tatattcttc taacttttcc ataagttcta ttcttgggtc
     2281 ttcttcttca tctttttgct tataaagcag atatttagac tttatctcta aaagttttga
     2341 tgccatagtt ataaattcac tagttatctc taaatccatt ttatctagca ttttcaaata
     2401 atttagatac tgcttagtta tatctattat agatatatcc tttatatcta ttttatgctt
     2461 tgttattaaa tcataaagta aatctaatgg tccctcatag acttgtaatt gtatattata
     2521 cttcatatca atccctcttt aatctgttta ttatattaaa caaataaata tactattttt
     2581 agcaacactg tatatattgg tgatagtatt atttgatata taccagtaaa taaagcaatt
     2641 aataaaatta tataactcat actttcaaat ttatacaaat attcattcca tttatatggt
     2701 acaaatgtag atataactcc ccatccatca agtggaggta tcggaagtaa gttaaaagct
     2761 gcaaatccca tactatatat aagcacatat ccagctattg tatttatagc atacatatgc
     2821 acatacttat tgataattac acacagtata gcagtcaaaa cattacaaaa aacgcctgca
     2881 agtgaaacta tcaggtttcc tatcttatag tttttaaaat tatttggatt tactggtact
     2941 ggctttgccc accctatctt aaggataaac atagccaaaa gacctattgg atcaacatgt
     3001 cttacaggat ttaatgtcat tctaccataa gctttagctg tatcatctcc caataagtga
     3061 gctgaataag catggccaaa ctcatgtatt gatattgcca tagctatacc aactaaactt
     3121 gctactattt ctcctatatt catacttaac attaatgtac atctcctaaa ttaaattttt
     3181 aatattatta taacaattaa atagcttata atcaaaatta ttttacatta taataatttt
     3241 aacttctagg cataaaatta aaatttgctt tttattttat aacttttatt attatatcat
     3301 attcattgtg aaaatataat aattacacct actgtaagtg taatattata ttgacatgaa
     3361 ttgacattat tgtctcttag tcaatattat acaagaagta taaaaaaata tgtgttacac
     3421 tttgtataac atgaaaggag gttacctaag tgagacaaga agaacgcaca gtatgctttg
     3481 atagggaatt aaaaattgaa gcatatagat ttaaaggcat aatgcaaaaa ttcccaaacc
     3541 attttcatga acattatgta attggtttta tagaaaaagg tcaaagatat ttatcttgta
     3601 aaaataaaga gtataccaca agtactggag atttgctact ttttaaccct tttgacagtc
     3661 atacttgtga acaaatagat gataaagtac ttgattacag atgtatcaat attaagccag
     3721 aaataatgaa aaaaaccgtc tttgaaatta caggtaaaaa ttatttacca aaatttaatc
     3781 aacctgttat atttcgcagt gagctagtac ctctactaca agaattacac tatataatca
     3841 tggaagaaga attggatttt aaaaaggaag agcttttttt cttcctaatt gagcaattaa
     3901 ttgaagaaca tacagagcca aatttgcaat ctaacttgga aaatacaaat atagaaattc
     3961 aagcagtatg tgattatcta gaaaataatt atgctgaaca tatagtactt gacgaattaa
     4021 gcactatagc aggtatgaat aagtactctc tacttcgtaa ttttacgaaa ttaaaaggta
     4081 ttacaccata tagataccta gaaaatatac gggtaaacaa agctaaaaag cttttagaaa
     4141 aaggcgtaga acctatagat gctgcgatac aaacaggatt tgtagaccaa agtcacttta
     4201 caaacttttt taagaatttt attggtctta caccaaagca gtatcaaaat atatttataa
     4261 atgatgattc aaacaacttt aaataaggta aaaaggagaa ataaaaatgg aaaataaaaa
     4321 ttcaaaatgt gtcatggtaa tagacgaaaa tttaccaatg ggaattatat ccaatacagc
     4381 agctataatg ggaattacac taggaaaaca tgccccagaa actgttgggc cagatgtaat
     4441 tgacaaaaca ggtaatagcc acttaggaat tattgacatt cctgtaccta ttttaaaagg
     4501 aaacaaagaa ataataaaag atttacgtaa aaaattatat actttggaat ttaacgattt
     4561 aactgttgta gatttttcag atgtagcaca aagttgcaat ctttatgaag aatttacaca
     4621 aaagattgct agtgttccag aagatgagtt acagtatttt gggatagcaa tttatgggaa
     4681 taaaaagaaa gtgaataaac ttactggtag tatgccttta cttagataat tgttaaacaa
     4741 ccctatatat gatattaaat caaatgatat tttaagatac ataaactttt ctatatttct
     4801 ataaaaatca taagattata aaatacttct atatggtaaa taaaaatacc cctatggcaa
     4861 ataactacat aagggtattt tttatgtgta ttataatcat atctttgtgt attataatta
     4921 tatttcatct gataattatt tatatcatat tatcaattat tctttgaagc atttgtagat
     4981 agcttgcctt atttatatcc ttagtattta tcaattccac ttcgcctatt acttttttat
     5041 ctttacatat ctttattttt cctaaaacag ttcccttttt aattggcatc ttaggatttt
     5101 ttactatttc aattttcttc tcaaattctt tagagcttcc ttttttaata agagcattta
     5161 aatcttcttt agcaaccaat ttaactttgt tttcatcagc cttatccaat gtaagtgttg
     5221 ctatattatc acctttacta cataacttaa cactttcata attagcaaat ccatagttta
     5281 aaaggctaga ggcatcatta aatctttctg gagaagtctc tgctcctaaa gttgcagcaa
     5341 ctagatgagt attacctctc tttgcagatg ctgataaaca gtattttgct tcttgagtaa
     5401 aaccagtttt aacaccagtt gctccttgat aatgctttat aagtttatta gtatttgcaa
     5461 gacctactgt aacttgtttc ttccctacta caaccttatc catccatgta gttaagtatt
     5521 tacttatcat ttcgtgtttt aatagttctc tagacattaa agctatatca tgagcagatg
     5581 tatagtggtt tgcaactgga agtccatttg tatttgcaaa atgagtatca ttcatattta
     5641 actcttttgc tttagcattc attctatcaa caaatccttc aacacttcca gctaagtgtt
     5701 cagccatagc aacacaagca tcatttgcag aagcaacagc tatacctttt aataatgtat
     5761 caaccttttg agtttctcct ggctctagga atatttgact accacccata ctagatgcag
     5821 tttcacttac ttgaacttca tcatcaagct taatcttgcc tgaatccaaa gcttctacta
     5881 taagtagcat agtcattacc ttagttacac tagcaggagg aagcttttta tgagcatcct
     5941 tttcatataa aatttgtcca ctaccaacat ccatcaaaat tgctgatttt gaagatatat
     6001 ttagttggtt ggcatcagca ttttccttat tatttgcaaa agaaaagctt aaaggagtaa
     6061 gcgcaattaa tatagccaaa gcagtactag ctaatttttt catcgatgtc acctcttaaa
     6121 gtttatttaa acttattttc tcacatttaa ctttttttat tctaaatgcc tagatatacc
     6181 ttgacctaaa ttttttttct tttcaagctt tttaaagtaa ccttctaaaa cacctttatt
     6241 agacgcattt tctattattt caaaagcatc ttcataattc attccatact ctcctgctat
     6301 ttccattttt gtttgtttta aagcttttat agcattttgt cttgaaacat catccataag
     6361 aatcctccta tcagtaaaat ttatatataa gtagttttta ctaataaaag attgttataa
     6421 ttggtatttt atgtaatata tatttttttt aaatgagtat taaaaagacc tcttgtaatt
     6481 cttaagataa tatcaattgg atttttgata ttactttaaa aattacaaga ggtctaattt
     6541 tcaaatttat agttttttta tattatactt ccattatgat aggaagtatc attggatttc
     6601 gtttagtttt ttgatataaa tattctttta ggttttcttt aatattattc tttaaataag
     6661 cccattcttt aatatttttc tcttcacatt catttagaac attcttaatt atatcctttg
     6721 ctccatccat taaatcttct gactctctta catatacgaa tcctctggaa attatatctg
     6781 gacctgctaa gaccttaccc tcatccttgg aaatagtaac tacaactatc ataagaccat
     6841 cttctgataa atgctttcta tcccttaaaa ctatattacc tacatcgcct actcctagac
     6901 catcaactaa tatgtttcct gtttgtatac ggtttgctac cttagcagag tttttatcta
     6961 attctaaaac atctcctgtt tgcatgacaa aaatattttc tttatccata ccaagctgtt
     7021 cagctatttc agcatgtctt tttaacattc tatattcacc atgagctggc ataaaaaact
     7081 ttggtctaac taatgcatgt ataagtttta attcttcttg acaggcatgt ccagaaacgt
     7141 gtatatcagc tatatcactg tatactactt ctgctccttt ttcaaataag aaattgatta
     7201 ctcttgatat taatttttca tttcctggta ttggatgagc agatattata actaaatcgc
     7261 cactctttat ttctactttt ttgtgctctg aaaaagccat tctagcaaga gctgacatag
     7321 gttcaccttg tgaaccagtt gttattatca ctaattcact atcttcatac ttacatatat
     7381 catttaaatc tataagcatg tcatcagaaa tatctaggta tcctaattct tttgcaactc
     7441 cgactacatt gaccattgag cgacctgata tagcaacttt tctattaaac ttttcagcag
     7501 tatttattat ttgttgtaat ctgtgtatgt tggatgcaaa ggtagcaact ataattcttc
     7561 tgccatttcc ttttgcaaat aattcgtcaa gacctacccc aacagtcttt tcagacatag
     7621 taaatcctgg tttttctgca ttagtactat ctgccagcat taaaagaact ccttttttgc
     7681 tcaactcaca gattctatgc atatccataa catctccatc aataggagtc aagtcaatct
     7741 taaaatctcc tgtgtggtat attatacctt ggtctgtgtg tattgcaata gagtaagcat
     7801 ctggtatact atggttattt ttcaaaaact ctacttccat attatcaagc tttataactt
     7861 gtctaggacc tataacattt aacttaacat tattcatttt atgttcttta agttttactt
     7921 gtataagacc tatactaagt cttgccccat atacaggtac atttattttc tttaatatat
     7981 atggaagagc tcctatatgg tcttcatgtc catgtgtaat aaatataccc tttattttat
     8041 ccctattttt tactaaataa gttatatctg gtataactat atctactcct aacatttcat
     8101 cctctggaaa actaagtcca gcatctataa caattatttc atctttatac tcaacaacag
     8161 tcatattttt tccaacttcg ttaagtccac ccaaggccat tacctttatc ttgttggtgt
     8221 tctttttgaa caattgcatc atctccttct gtctatttaa ttttttaaca cgttctacaa
     8281 tcataaataa tatcaaatca cgcactcgct accagttatt cttattgtac cataattcgt
     8341 atcatatttt aagctatatt taaaatacat ttaacgtatt attttgacaa acctatttta
     8401 ttttgatatc ctctaaatat tatatgattt aatatcggat aatttaggag gatttatgcg
     8461 tggtaaaact cattgtgcaa taggaatctt aacagctatc caaacatctt taatatttaa
     8521 aattcctata tctttagttg atattttagt ttcagctact tttgctgtac tacccgattt
     8581 ggataaatct aattctatgg tctcaaattt cattctaaaa aacaatgtat ccaaatacat
     8641 atatagaatt tttatatatg ctgttaatat tattattttc tttatttcaa taaatataaa
     8701 tgataatttt tatttaagcg caataattac atttgttgca attataatta ttgaagcaaa
     8761 actaacccac acatttctaa gaaaagtttt tctatcatta atatttatac tattagctat
     8821 atgcttatac atcatagaag ttgaaatata ttttacaatt ttttgtctaa tgttgtcaat
     8881 ttttccctgg ttaaagcaca gaagtttttc tcatagcata tttgcaacta taatagtata
     8941 ttttttacta aaacagatag aattaatcac taatattaat aatttatctt tttatggtac
     9001 gattggttat gcaagtcata tgtttttagg agatttattt acaaagcaag gaataccaat
     9061 tttttatccg ctaagcgaaa agaaaatttc tttaggtttt ttaactgttg gaggaccatt
     9121 tagtaatttc atagaaaaat cattcatttt tgtacttata ggattaatta tattttcaat
     9181 attaaaacta taagcatctt gaaaactgta tatttgcatt ttgtagatgg tttataataa
     9241 aaaatggaag aatagcatag aatataaaag ttctatacta ttcttccatc gacattaagc
     9301 aatagaagtt tattcttctg aatcgcttac tccattacac tcgtcacata atccataaaa
     9361 tttaacatca tgatcaagta ttttaaattt gtacaatgat tgtatctctt tttctatatt
     9421 gtctaaaagg tcttctttag cttccattat ctttccacaa tttttacaaa taagatgatg
     9481 atgattatga cagtcttctt tatgtagact aatctcatat cttatacatc catcgtctaa
     9541 gtttagtttt gatattaatc ctatttcatc caatagctgc attgttctat atacagtagc
     9601 taagcctatt tcaggacagt caaccctaac taaatcatag atttcctcac tacttagatg
     9661 tgaatggtca tgtttaagca atatttcaac tattgctctt ctttgtggcg taatcttaaa
     9721 accagtttct tttaacttgt cttttaataa atccattgta tttgccataa catcaccctt
     9781 actattttca attgataatc tttattaaat aatattgtaa ttgataattc attttttgtc
     9841 aagacattta aataaattta atttattctc ataatataaa tatataccca taaaagatat
     9901 attattcacc aaaattcata aattttttgg tatttttaat aaaaaacaaa aaaacagata
     9961 tatactataa atgcttttac aattaataag gtttaattaa agcaaatata ataaatatct
    10021 gatttttata tacaaactta attacttatc tagttaagtc catttgattc atcttcgtcg
    10081 taatttaaac cttcttcttc cattatagca gtatacacag ctacaacagt ttcatactct
    10141 tcatcacttt ccaaaggtat taatatttca ccttcttcat cctcatcaat tctaaagatt
    10201 aaagcttctt cagcttcttc atcatctaaa ggcattaata ttgcatactc ttttccttct
    10261 gcttctaatg ttaatattat ttcaaattgg ctttcaacgc cattttcgtc tattaaattt
    10321 attatatttt cttccataat gtcattcacc tcaaatttat ttttattttt attatttata
    10381 ttatatatca taagtattca attttatagt tcttttatga atttatttta aaatctaaat
    10441 atccttgcaa aattattact gctgccaaca tatctataac cttttttctt ttttgtctac
    10501 taacatctgc ttctaataaa gacctctctg ccgctactgt agaaagcctc tcatcccaaa
    10561 actctacatc aatacctgtt tctgctttta ccaattcaca aaacttgatt actttttctc
    10621 cttgtggacc taaagttcca ttcatatttt ttggtaatcc tgatacaatt ttatttactt
    10681 gtttttcttt tattattgct tttaactctt caatatcttt tttcttgcca actcttttta
    10741 ttgtttttac accttgtgca gttaatccca tcaaatcact cacagcaact ccaatagttt
    10801 tatcaccaac atcaagaccc attattcttc catctaacat agttcctcct ttatctattt
    10861 atattctata gattaaatac atattctgaa cctctcataa ctaaggtcac aggcttcctg
    10921 cttcatagaa ttttgcataa tcaatcataa aaattgcaat atgatttttt atgtaagcat
    10981 ccagtttaca ttaataatca tattgcattt tttatttaaa tcctatatga acagtaaaaa
    11041 attttttagt tgtttttttc aaatattatc atatttatat ttattattat tagtggtata
    11101 agtatatata tagatgctgt agatgcagca actattccag aatcattaac taaaagagta
    11161 attatacaac caaccataga tgcaataaac cctttaaaca agattggaaa attattcatt
    11221 atctttctaa agtgtctaga tggtttaaat ataagtactg caattactcc aattcctaca
    11281 agtaaaatat ttacccatac acttgtctgt gctagttgca tattcatttg aatttttctt
    11341 ccaaatgttt gtaatatctc tcctggtcca tttaggaaaa ttctctgtac aaacatacct
    11401 aaatgagatt cactacctga aagtatatct aaagctgcaa atgcaaaaac gacaactaca
    11461 gctgaaacag ctaataatac agctttctta aaatctattt taatgtcata tataagcatt
    11521 ataaacaaca aatatgctac acattctgat atagctccac caacatttgc acccattgaa
    11581 ggatatgcac tagttataag tattattaac gaaaaaatta caacaagtaa tttagatacc
    11641 ttcttataat gtaataatac tgctaagcca aatactgcac ttgctatagt tacaccttca
    11701 tattcatttc ctacaccata gtacctagct cctatgacag catcataact cattatgtta
    11761 ttttgcatta agtatgttcc aaatacagaa tctataacta tgactaaaat agtaagtgtt
    11821 gcaaaaaatc ccatgtgctt tatatcattg tccttaaata attttctacc taacaagtat
    11881 aatattagcg tagtcgcaaa tattcccaaa gatattgaaa ttggagtttt aaaattcatc
    11941 aaaggagcta acataaatga tagtggcatt attatgccta attttataaa ctccttaagt
    12001 actttgaata cacgctcttt atgtggcata taatttctaa agaaaatcgc aaacatggca
    12061 attacccaag aagcagatat tacgcctaca aagccattta taatagttga ccttattgaa
    12121 gatatagaca ctatcctttc ataaagtgat tttagatagc caacattgtc ctctttattt
    12181 actaaagtat aacttctacc taccatctct ttattttcaa gaccaaatgt acctagtata
    12241 tctactccaa catctaaatt ggcaacaatt ccagcctttc ttgttgtggc agattccagt
    12301 agacctttcc cttctccact aaattttatt attggagaaa gtcttctctt attcttataa
    12361 tctaggtcac ttggaaatgc acttgctata taaacagtgt cattttctcc aaccatagaa
    12421 aatacttgtt caagatattt atttatattt tttacaatat ttttcttcat tgtaccataa
    12481 gtattatcat ttagatttaa tctatacata tctaatctat atgtatctcc aagttctaca
    12541 aatatagcat cactattttt ataggatttt ttagtttcat ctagtaattt atcgtagtct
    12601 gtagatattc caaatggcat tttagaatct ttcttattaa tattatcaat attacctgaa
    12661 tcaatccttc catattggtc catagctact aaacataagt ttctattttt aactaattga
    12721 ttattttcaa ttatatcagc atttccaatt acagatgtct ttaaaccatt ttctgataaa
    12781 gtttttccta atgaccctaa atttgctcca tattctccat actcaagatt ttcatttata
    12841 gatctattaa tgcctaaatc atttatttct ttagcttttt gacctgttgc gacttcaaaa
    12901 gctttagcag tatcttggtc tactttttga aaattgatat agtcttcagt agttacacta
    12961 gctcttgttc ctgcacccat acttgcataa gaccttctat catcagtacc ttgatcccct
    13021 cttatattca tgagagctac atagcctctc ttttctatct catttttcaa gattggaata
    13081 ttcatcatac ttccaagatt ggtcctattc atatcaataa atatgacctt gcctttttct
    13141 tcagcaaaac catagctagc agttgataca aaaatcataa tgactattgc aaacaacgac
    13201 ataatctttt ttctcattta tttgacctac tttctattca gatagtgcgt gattacttct
    13261 tcaagtatct catctctttc aaatttttta attatagctc ttgcattttt atgactagtt
    13321 atatagctag aatcaccaga aaggagatac cctataattt gatttatagg gttatatcct
    13381 ttttcaacta atgctttata tacaaaatca atagtatctc ctacactcat cctatcttct
    13441 ggtattcctt caaatttcat agtgtagtct aaatcttttt ccaaactaaa aacctccttg
    13501 agtgaatgtt atttaacttg tgtcttaata acctcacttg catagcttaa agcttggtca
    13561 accttagata catcactagc tcctgcttgt gccatattag gtcttcctcc accttttcct
    13621 ccagctattt ttgcaacttc tctaacaata ttacctgaat gtattccctt atctaataca
    13681 tctttagtgg cagtaactac aaagttaact ttatcatcta ctacatttgc aagtactact
    13741 acaccagaac caagcttatc tcttagatta tctgctgttt ctcttagtgt atccatatcc
    13801 ataccttcaa atttattagt tatcaaatta actccattta cttcaacttt agaatcaaaa
    13861 atagaatcaa ctgattgtaa actcatctta gctttcatat catgcaattc tttagataag
    13921 tttttatttt cttctaataa agaacttatt ctttgaatta aattatcttc tttagatttt
    13981 aagtttacac aaacttcaga gataattcca tctctttctt ttaggtattc ataaacagct
    14041 tttcctgtta ttgcttctat tcttcttaca cctgctgcta ctccaccttc agataatatt
    14101 ttgaacattc ctacttgaga agtatttgtc aagtgagttc ctccacaaag ttctgtagaa
    14161 taatctccca tagaaactac tcttacctca tcaccatatt tttcaccaaa tagtgctgta
    14221 gctccttttt ctttagcttc ctttatattc ataatatcac attttatgtc taaagatgat
    14281 aaaataacat tatttacttt ttcttctatt acttttaatt cttcatttga tatcgcttca
    14341 aagtgagtaa tatcaaatct aagtctttct ggagttacta aagaaccagc ttgattaaca
    14401 tgttctccta atacttctct taatgcctta tgaagtaaat gtgtagcact gtgatttctt
    14461 gcagctgaca ttctagtttc tctatctacg agtgtcttta ctttgtcccc attacttatt
    14521 cttcctgatt ttattatacc tatatgtttt atactattat tagcaccttt tttagtattt
    14581 aatacttcta aaactaagtt ttcattagtt atcaatccac aatctccaac ttgtcctcca
    14641 ccttcaggat agaaagtagt gttatctagt actatagata ccttatcgcc ttcttctgca
    14701 ctttgaacta gctcatcatc tttaactata gcttcaatag ttccttcacc ataaatttca
    14761 ctatacccat taaatgtact gtcaactgta gattctaatt ttgatagagg gtcttccttc
    14821 caactttctc catccatatt tcctctagca tttctagctc tttctttttg cttttccatt
    14881 tcttcattaa aagcttcttc atctacagat agatgttctt cttctaatat ttctttagtt
    14941 aagtctatag ggaagccata agtatcatat agtttaaaag cttcttgacc acttaggaca
    15001 gtctttcctt cattttttaa tacttctata taagagttca atatttctgt tccttggtct
    15061 atagtttcat tgaatttttc ttcttctatt ccaactactt ttttaatata actttctttt
    15121 tcaactaaaa ctggatatgc tttatcattt acctttataa cttcatctat tagtttctgt
    15181 aaaaataatt cttttactcc taataactta ccatgacgag cagcacgtct aaggagtctt
    15241 ctaagaacat atcctcttcc ttcatttgat ggaagcactc catcactaac taagaatgtt
    15301 acagctctta tgtggtcagt tattattctt attgatatat cattttttgg attctctcca
    15361 tattttactc ctgttaactt ctcaactgct tctagtatag atttaattgt atcaacttca
    15421 aagatagtgt ctacgccttg cataatacat cccattcttt caagacccat acctgtatct
    15481 atattcttgt tttctaatag actataattt ccttcttcat ctctatcaaa ttgagtaaat
    15541 acatggttcc aaaattctaa atacctatca caatcacagc ctggtttaca gtctggattg
    15601 tcacatccat attcttctcc tctatcaaaa tatatttctg aacaaggacc acaaggacct
    15661 gtacctattt cccagaaatt atcatctttt ccaagtctaa ccattctttc ttctgggaaa
    15721 ttcatttctt ttgcccatat atcataagaa tcatcatctt cttcatatac agtcacccat
    15781 atcttatctt ctggtatatt tagccattct gtaacaaatt cccatcccca tttgattgac
    15841 tctcttttaa agtaatctcc aaatgagaag ttacccatca tttcaaagaa tgtagcatgt
    15901 cttgcagtta tacctacatt ttctatatct cctgttctta tacatttttg agatgtacac
    15961 attctcacac ttggaggtac ttctacacct gaaaagtagt ttttcaaagg tgccatacct
    16021 gagtttataa gtaacaaact cttatcattg tttggtacta aagaataact atttgctaca
    16081 taatgacctt ttgactcaaa aaattctaaa aacttacttc ttatttcatt taatcccatc
    16141 ttttccatat catgttcctc ctaataaatt ttattttttc gtttaaataa aaaacctcgt
    16201 ccctataaat aaatttatag gggcgaggac aatcgcggta ccaccctaat tatcatacaa
    16261 agtatgatat cttcggaaaa ccttagctat aacgtaacta ccgtcaatct ctacttaaat
    16321 cttctttata aaagataaat tgttcaagat tgctactaaa aggctgcttc aaatatcata
    16381 ttctaagggc ttccagcatt tccctctctc tgtaagacat caaatatttt actattcctt
    16441 atcactgtat ttgtttttta attaatatac taattatatt actaaatttt acaaaattaa
    16501 tcaatactat attttaatat acaataaatt tattttataa actttattta ctcttaatat
    16561 actttattgc ttattttttt aattttttag atgtttttat aaacttttct cgattatacc
    16621 tccacctact acaatatctc catcatacat tactacagat tgacctttag taatagctct
    16681 ttgagcttca tcaaaaacta ttttaatagt atcttctcct actctatgaa tagtagctgg
    16741 tgatggtttt gctgaatatc ttattttggc ttgaactctt aagggttctt ctaacgtatc
    16801 tattgatata aagttaacat ctttagcaat aagttccttt ttaaataaat cttcattgtc
    16861 tccaagaact atggtattat ttattggatt tatgtctatt acaaacattg gttttccaaa
    16921 agtaattcca agcccttttc tttggcctat agtataatat aatataccct tatgttttcc
    16981 aagtatattg cctttagtat ctacaaaaaa accttcttca atacgctttt tagaatgttt
    17041 ttttacataa tttgcatagt cattatcttt aacaaaacaa atttcttgac tatcaggctt
    17101 attgtgtact gccatcccca tttccttagc aatttctctt actctatctt tttcatagtc
    17161 acctattggc aataaagtat gctctagttg ctcttgtgta agattataaa gtgcataagt
    17221 ttggtctttt ttatctgtaa cagatttttt tagcaaatat cttcctgtac tttcatcctt
    17281 ttctatcttt gcatagtgac ctgtagctac ataatcacaa cctatttgtc tagccttctt
    17341 ataaaaatca tcaaatttta tatgtttatt acaagctata cagggattag gagtttttcc
    17401 ttccaaatat tcatctataa aataatctat tactttttct ttaaacactt ctctaaaatt
    17461 taaaacataa aatggtatac ctattttatt tgctaccctt ctagcatctt caacagcaga
    17521 taaagagcaa caaccacctt caatttcaac aacatcatca tcttgccaca acttcatagt
    17581 aacaccaatg acatcatatc cttgttgttt taaaagatat gcagctacag agctatcaac
    17641 tcctccactc atacctatca ttactttttt attcataaac aaatttcacc tcatcttttg
    17701 cattacttat tattatacca tatagagcaa gtattgtact tatttaaaat atagcttgtt
    17761 ctattttcta aaaaaataat catctttatt atttgaatct tatggattat tttgttaact
    17821 aattaacctc taaatcaagt taaaatactt aatttaggca ataaaaaata gccattttaa
    17881 aaacagctat tttttatgtc ctaattatat cattagtact tctttttaat cattgtaaac
    17941 ttctcataac acaatcactt atttttattc ttcttcttca acatcatggt catgagcatc
    18001 atctataaca tatccatcca attctggtat atggatattg ttcttttgag cataatctat
    18061 aagagctgct tttacagctt gttcagctaa aactgaacaa tgcatcttta ctggtggtaa
    18121 accatccaaa gcttctgcaa cagctttatt tgtaagttct aatgcatctt ttatagtttt
    18181 tccttttatc atttcagttg ccatagaaga acttgctata gctgaaccac agccaaatgt
    18241 tttaaactta acatccttta ttacatctcc atctatgtct aaaaatatct tcattatatc
    18301 tccacatgta gggtttccaa cttcacctac accacttgca ttatcaattt ctcccatatt
    18361 tcttggattc ataaaatgtt ccataacttt atcactatat tgcattatct tttcctccta
    18421 ctcaatttat gtctattttt tatgctttat ctttctgtct attttactaa accttgtttc
    18481 ttcgcatcat cataaagtgg tgacatgctt ctaagtcttt ctattacttt tggtaagttc
    18541 tctagtatat aatctatatc atcatctgtt gtaaaatctc caactgtaag tcttaatgaa
    18601 ccatgtgcta tttcatgtgg taaacctatt gcaagaagta catgtgaagg gtctaatgaa
    18661 ccagaagtac atgcagaacc acttgaacca gcaattccta acatatctaa taataaaagt
    18721 attccttcac cttctataaa ttggaatgca aagtttgcat ttcctggcaa tctattttct
    18781 aagctaccat ttactcttgt gtaaggtatt ctttctaaca caccatctat taacttattt
    18841 ctaagacgag ttaaagtttc tacatgattt tgcatatttt ccttagctaa ttctgctgct
    18901 tttccatatc caactattgc aggtatattt tctgtaccag ctcttttgct tttttcttgt
    18961 gctccaccat ggacaaagtt atgaagtctt acaccttttc ttatgtataa agctcctaca
    19021 ccctttggtc catatatttt atgtgaagac atactcatca aatctattcc taattcttta
    19081 acatctactg gaatattacc tgtagcttgt actgcatcag tgtggaataa tatcttatgt
    19141 tttttagcta tttcggatat ttctttaata ggctgtatag ttcctatttc attatttgca
    19201 aacattatgc ttataagtat agtagtatct tttattgaat cttctagttg tcttaagtct
    19261 actcttcctt cgctatcaac atctaaataa gttatttcaa atccatgatg ttttgctaag
    19321 tattcacatg tatgaagtat tgcatggtgc tctatttttg aagttattat atgattacct
    19381 ttgtttttat tagcataagc tacaccctct agtgtccagt tatcactttc tgaaccacct
    19441 gctgtaaaat atatttcact aggttcagca tttataaggg ctgctacttg ttctcttgct
    19501 ttatccaaag catcttttgc ttctcttccg aatgtgtgaa aactagatgc attaccaaaa
    19561 taatctgtta ggtatggcat cattgcatcc aatacttctt ttttaatagg tgttgttgca
    19621 gaataatcca tatacaatct tcttttttcc attacattca tctccaaatc tattttttaa
    19681 tatttgttat atcattttct aatttatttt tgttatagtc atttatcata tctttaagag
    19741 taattgaatc tataacatct tctataccct ttttcatctt ttcccaaact atttttgtta
    19801 cacacatgtt tgagttttca catacatctt catctagtac acagtcagat aatgcaactg
    19861 gtccttctaa tactacaagt atatctccta ctgttatgtc ttcagcattc tttgaaagca
    19921 aataacctcc ttgagcacct ctcacacttt ttactagacc cgattttttt aaagatgaaa
    19981 atatttgttc taagtactga tctgatattt tttgcttctt agcaataaat tttaatgata
    20041 ctggtccatt gtcttgattt aatgctaatt caaacattgc ttttagtcca tatctacctt
    20101 tagtagacaa cttcatatat tatcacctct atatagaaat ctaattcata ctgttttagt
    20161 aggaattact ttcaagataa ttttattata ccctactaat tttgtcaaga ttaattttaa
    20221 ttttttattt tgaataaatt ccccattttc caatgttttc aaatcctagt tttttatata
    20281 tctttccagc ctcttcatta tcataaaaca aacaaggaat tttgttttct ctcagtaact
    20341 ctgaacatag tttaattagg cattttgtag caagtccttt agctctatat ttaggatgtg
    20401 ttcctactcc aattatcatt gcatgagtcc tattttctga agtactcttt gccattgaca
    20461 ccacctgttt gttaatctct atacaatatc ctctacctgt ccttagtcca ttttttaaat
    20521 tctctaaagt tgtactgtga aattcatcta ttaagtcata aagtttcact gtctttttta
    20581 gatttccaag cctaattctc ttaacctttg cattacaatt attatctatc aaaaactttt
    20641 tagtttgaag tttgcaaaaa tcaactacct ttaaattatt aagtcctaat ctctttgcta
    20701 aagtatctac agcacatatt ttcccagata tttcttcgta attagtatta cgaagtagat
    20761 tataaaaacc ttcaacatca tactctccat cagaataaaa catcataaat tcaaaatatt
    20821 taagtaatat tgcctttatt tcaccatgtt cacccacgtc tgcccatata tttaaaaaat
    20881 tatttccata tccatatctt tcaatatccc ctattataaa caaattgtac tcaggttctt
    20941 tttttaagta tttcataact ctactgtgat aagttgagtt aagttttata atcataatat
    21001 ctacccccga tacataaaat tcagtctaaa aatcactatt tagatattct aaatattttt
    21061 ttatattttt ctcagtttta ttttcttgtg gtatgtaata aatactatct tttatgctat
    21121 ctggtaaata ctgttgcttt acataatgat ttttaaaatt atgaggatat ttgtatccac
    21181 aacccctacc tatttcttta gaaccagaat agtgagcatc ctttatacta tttggtatat
    21241 ccttaacaaa ctctttatcc agttcatcca tagctttcat tattgctata catgcagaat
    21301 tagactttgg agatgtagcc aaaagaatag tagcttctgc taatggtatt ttcgcttctg
    21361 gaaatccaag ttgcatagct gagtctacac atgcctttac aattacaata gcatttggat
    21421 aagctaaacc tatatcttca ctagctatta ctaaaagtct cctacatata cttatcaaat
    21481 ccccaccttt tataagtctt gctagatagt gtatagatgc ctgtgggtca cttcctctta
    21541 tagatttttg aaatgcactt aaaatatcat aatgactatc accatttttg tcatagttta
    21601 taattttatt aaatgtagat gcccttatta catctgaatc tatataagtt atattatcct
    21661 tatttggttt agttgaatag actaccactt ccaaaatatt taaggctctt ctcatatctc
    21721 catcagaaag aattgctata tcttcaatag cattattatt gcactcaata tccatatagc
    21781 tatcttcatt taatacttca acagctcttt tcagtccttt ttctatatca agtttttcta
    21841 aaggtttaaa ttcaaataca gtagacctac ttaaaagagc tttatataca taatgatatg
    21901 gattttctgt agtgctagca atcaaagtta tactcccatt ttccataaat tctaatattg
    21961 attgttgctg ttttttatta aaactttgta tttcatctat atataatagg actccattta
    22021 cattatttat acttccaagc tctgatatca ccttttttat atcttctaaa gatgaattag
    22081 tagcatttat tttatagaag cttttattgc ttctttctgc tattatttcc gctactgtag
    22141 ttttaccaac accaggagga ccaaaaaata tcatatttgg taaaaaatta gattgtaata
    22201 tcttagacaa tatttttcca ttacctatta tatgactttg acctattaca tcattcattg
    22261 tttttggtct tattttatct gctaaaggca ttttctcact cctttttaat atacttatca
    22321 ttaccttaat aattcgtcta aaaaaggtat ctatttgaaa tagatacctt ttctaatcta
    22381 aacctataaa atatagatta ttgattgttt tttaattttt ttaattcatc tgtaagtttg
    22441 tcattaagta ttcttatatg agtacctttc atacctaatg atcttgattc tatcacacct
    22501 gcactctcaa attttctaag tgcatttact attacagacc tagttatacc aactctatca
    22561 gctatcttac ttgctacaag tagaccttct tttccatcca attcagcaaa aatatgttca
    22621 actgcttcaa gctcggagta ggacagagtg cctattgcca tttgaactac agctttcttt
    22681 ctcatttctt cttctaattc ttcacctatt gctcttaata tttcaagacc aacaacagtt
    22741 gcactgtatt cagctattac taaatcatca tcattgaatg aatttgaata tcttgaaagt
    22801 accaatgttc ctaatctttg accgcttcct aatattggaa ctactgtagt atatttttgt
    22861 aatcttccat gttcttcagg aaagatttct aatatcttct caccatttaa attttctaat
    22921 gtttcatcaa tttttaacac attttgagta tattcatctg aaaatttctt ttgcttagta
    22981 tactcatctt ctataactga actgtcttga acatcattta aatgaagacc tagtacttta
    23041 ccttttgcac ttactacata aacattagaa cttaaaacgt cgcccaatgc tccggccagt
    23101 aaatcaaaag agacactgct tccaccactt gtttgtaatg ttttatttat tttccttgtt
    23161 ttttgtaaca cttcacttgc catttaatca tctcctcatg aaatttaggc cttttaataa
    23221 ccttttatat tttctaatat tattttcaca tttaatgaaa atttattttt ctattagttg
    23281 tttacacttg aaagattatc atagattgga acctttttca agagaattat gtgatttttt
    23341 tcaattttca ctcttttccg tcattatgct cttttgtctc tttattacat tctttattag
    23401 aacacactgt ttttgtttca gatttagtca ctttttcaac catatatgaa ccacacttct
    23461 cacaaaactc tcctgttggc ttattccaag ctacaaagtc acagtttgga taattagaac
    23521 acccataaaa agctttacct cttttagatt ttctaagtat aatatctcct tcttcacatt
    23581 ttggacattt aacaccaatt ttattcataa ttggtctagt tgttttacaa tcgggatagt
    23641 ttttacaagc cataaacttt ccaaatctac catatttgat aaccatatta gacccacagt
    23701 tctcgcaaat ctcatcagtt tcttcatcca tatttacttt ttctatattt tcaagagctt
    23761 cttcaatagc ctctttaagt ggtgcatatg attctgatac aatctttttc caatctgtat
    23821 tgccttcttc tataacatca agtttacttt ccatatcagc agtaaaatct acatctataa
    23881 atttatggaa attattttcc aaaatatcag taactattat tccaagttct gttggacata
    23941 gacttgttcc ttgcttttca acatactctc tattcagtat agtagctatt gttggagcat
    24001 acgtacttgg tctacctata cctagttctt ctagtgtttt tactaaacta gcttctgtat
    24061 atcttgctgg tggttgtgta aaatgttggt ctggcaatat attttctata tttaatatat
    24121 ctcctttttc tatacttggt aaaattttgt cttctctttc tgaaaaatta tataccttgg
    24181 tataaccatc aaatttcatt tttgaaccag ttgccttaaa tacaatatcg cctattttac
    24241 attctacatt tagtatatca aacactgaat cttccatttg actagctaca aatcttctcc
    24301 aaattaaatt gtataacttg tactggtctt tgctcaatga ggcttttata ctctctggag
    24361 ttctatcaac tgaggttggt cttattgcct catgggcatc ttgaaccttt ttagtatcct
    24421 ttttttcttt tttatcacca gtgtttttat agtagttttc tcctaaattg tttacgatat
    24481 aacccttagc tttttctttt gcttcatcag atattctttt agagtcagtt cttatatatg
    24541 atataagacc aacagtacct tcaccttcta tgtcgatacc ttcatataat tcttgtgcta
    24601 tcatcattgt ttttttagta gtaaaaaata acttatttgc tccttcttgt tgaagcatac
    24661 ttgttgtaaa tggttttgga gctgatttac gtcttgattt agattctatc ttctcaacct
    24721 ttaaatcttt accttccatt tcttttagta tttcatttac agcttcttca ttttctaatt
    24781 caatcttttc atcattttta ccataaaatt tcaaagttat atctttgtct tttactgtct
    24841 tagcctcaac atctattgtc cagtattctt ttggaataaa tgcctttatt tctttttctc
    24901 tatcacaaat taactttgtt gtaacagatt gcactctacc tgcactaaga ccttttctaa
    24961 ccttttgcca taatatagga cttatttgat aacctaaaag tctgtctagc actcttcttg
    25021 cctgttgtgc atcaaccaaa tttatatcaa tatttctagg atgtttaatt gcttttttaa
    25081 ttgcatcctt agttatctca ttaaactcta ttctacaatc ctcagattca tctaaattta
    25141 gtatatgagc taaatgccat gatatagctt caccttctct atcagggtca gttgctagat
    25201 atacttgttt agatttttta gcttcttttt ttaattcttt tattacatcg ccttttcctc
    25261 ttatatttat atattgaggt tcaaaattat tttctatatc tacacctaat ttacttttag
    25321 gtaaatctct gacatgacca actgatgctt taactgtgta atgacttttc cctaaaaatt
    25381 tttctatagt tttagctttt gcaggcgatt ctacgatgac taatgttttg gccataaaac
    25441 catacacccc cactacctaa tcttaattta tacatttaaa ctgtacgttt tattattcat
    25501 ttctactact aactcattta gtactaattc attgattatt gaatttacat actttatttc
    25561 tataccagta taatcacaaa tactatctat atgcaagttt ccttctcttt ttatagcttc
    25621 aattatttgc ttagacttgg catttagact tatattatca tatttttgat ttattttttt
    25681 atcaataata ttaaatatgt tatattcatt taaaatatca tctatatttt caattaattt
    25741 tgcaccttct tttataattt tatgacaacc tctactcatt tgagaattta tattaccagg
    25801 tattgcaaaa acattttttc catgttcaag agcaaaatct actgtgatta gtgcaccact
    25861 ttttattgct gcttctacaa caactactcc atcacttaaa ccactgataa ttctatttct
    25921 attagaaaaa tggtacgggg ctactgtcga gttaatatta tactctgata ataataatcc
    25981 cccattttct aatattttat ttgataaatg taaattttgt tttggaagag gattgtctac
    26041 tcctgaacca agtactgcta tagttttaga ttttccactc atacaaccta tgtgcgagta
    26101 tgcatctatt cctatagcta atccacttac tatattaact ccatattggg atagttgaca
    26161 acttattgtt tttgcacaat taattccata ggctgtaggt tttcttgaac ctactatagc
    26221 tatattaaaa ttattattaa ctaaacctat gtcacctttg tagaataata actttggagc
    26281 gttgtatata ttctttaaat tttcaggata ttctttatca tatttacaaa tatatttaat
    26341 cgcttttttg taaagtagtt cttttatatt ttctaaataa gcgtaacctc tatattttac
    26401 tatatttttt ctaatattca agcttatatt ttttaaatta tatatttctt tttcagaaaa
    26461 atcaaatata tcttcaatat ttactatttc attttctatt atttcaattg tttttgttgt
    26521 tattccacca attgatttta accataaata agtatctctt ttttccataa aatccacctc
    26581 aaaaaactat ttaaaataac tataataagc ctttctaaat gaaaaagctt ctataataca
    26641 ctgtgaattt atcaagtctc tttcttctaa atctgctatt gttcttgcca tttttaaaag
    26701 ttttgtataa cttctactgc ttaggttgta tttattaaat atcatctcag ccgtttttga
    26761 agcttctttt tccagtttac aatatttaaa taaattgtaa gctttaattt cactattatt
    26821 ttttatatta tctttcttaa atctatttat ctgtattttt ctagcatttt ctactcttct
    26881 ctttattttt tgagagcttt cttcttgttt taaagaatta aaatctgaat attttataga
    26941 attaacttct acaaaaatat caaacctatc taacaatgga cctgatattt tatttatgta
    27001 tctatcaatt tcgttacttc tacatctaca ctctgtctct gacatataat aaccacatgg
    27061 acatggattc atagctgcaa ctaacagtac tctacaagga tattttgcac tgtactttac
    27121 tctggatata tttacatatc catcttctat aggttgtctt aaagtctcaa gtatttttct
    27181 atcaaattcg gctatctcat ctaaaaataa gatacctcta tgtgctaaag caatttctcc
    27241 aggtcttgca tccattcctc ctccaattaa agattgcttt gtagttgtat gatgaggtgc
    27301 tctaaaaggt cttttatcaa ttattccttt actttcattt atcataccca atatactata
    27361 aaccttactt acttctatca tttcttctat acttatatct ggtaaaattg tccttactct
    27421 ttttgcaatc atagtttttc ctgagcctgg aggtccaatc ataaacatat tatgatttcc
    27481 agctgctgca atttctgcac tcctctttac aaaataattt ccttttacat ctttaaagtc
    27541 ttcatcatat ttactttctt cattattaat cttagtatat ttgcaatcta tataactatt
    27601 atctttttct aattctccgt tctcttttct tgacttatca tccaaaaaag acattatact
    27661 tactttatcg acttttatct cttcgtttaa aaaatcgaca cattctttta aacttttaat
    27721 aggaattata tcaatttcat ctactaatag actttctttt atgttctcaa ttggtataaa
    27781 aatacgtttt atgttctgag tttttgcacc cataattatg ggcaatatcc ctcttacttt
    27841 tcttattttt ccatctaatg ataattctcc tataaacata ctttctctta tgtaattttc
    27901 atcttttttt attaactctc ttaaaaggcc tatacttata gataaatcta aaaaagctcc
    27961 ttcttttttt atatctgctg gtgataaatt tacaactatt ctcgtgcttg gaaatttata
    28021 tccactatta agaattgctg actttactct ttctcttgct tctttaattt ctttgcctgg
    28081 aagaccaaca atattaaaac taggtattcc attgcttaca tctacttcaa ccttaactaa
    28141 aaagctgtcg attcctacta agttgcttga attaataata cttaacatat ttaatccttt
    28201 ccaaaaacat tatctttttt aaaaagcatt cattatgtga tttattcttt tttctaacaa
    28261 atagacctct atgatgtcaa atcttatggg agtattattt aagttatttt ttataatata
    28321 gtattttgcg acaccaatta tcttttttct ttttttaaaa tctacagatt cacaagggta
    28381 gccatacttt atgttagacc ttgatttcac ctccacaaat acaacttcat ctcctatatt
    28441 tgctattata tctacctccc ctatccttaa tctataattt ttttctagta tctttgcacc
    28501 ttttgatagc agataattaa ctgctatttc ttctccaaaa tcaccttttt ctttattatt
    28561 cattatctac tccaatatat taaattttta cttttaaatt atcgtcagta tacaatttaa
    28621 ctattcaatt ttttgaaatt gattttattt ccattgatat taataaatac ttctattgac
    28681 tttattcttt tatgatatta tgctagtttt tataatgaaa gtttattttt atggaggaat
    28741 gtctttaatg aacagaaaaa aaacatttat ttttatacga tatttatctc tatttatatt
    28801 acttataagt tatttaaacc atactgaaaa tacattgtat ccagctataa caatgattat
    28861 attatttcta ataataaata atcaagttag atttttttct ttatcaagtg agaataaatt
    28921 tatacttatt tcttattttt tagagcttat tctaattttt atattttgta agtatataaa
    28981 gacttttaat ccacttta
//
LOCUS       DAELSY010000033        28641 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.033, whole genome shotgun sequence.
ACCESSION   DAELSY010000033 DAELSY010000000
VERSION     DAELSY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28641
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.033"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(634..1227)
                     /locus_tag="KPG09_001352"
     CDS             complement(634..1227)
                     /locus_tag="KPG09_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0965973.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
     gene            1788..2018
                     /locus_tag="KPG09_001353"
     CDS             1788..2018
                     /locus_tag="KPG09_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965974.1"
                     /translation="MRLDMDSMLYVLIYMTLLVSIIGSIFYFCKDLLNHKIKYVLIRD
                     IFVNRITYSEERLPKQYIVFQKYDIWRYCSLF"
     gene            2075..2725
                     /locus_tag="KPG09_001354"
     CDS             2075..2725
                     /locus_tag="KPG09_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG0965975.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRMYFNRKNRIVTK"
     gene            2939..3610
                     /locus_tag="KPG09_001355"
     CDS             2939..3610
                     /locus_tag="KPG09_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0965976.1"
                     /translation="MAKILVVEDEKRMQSIIVEYMQKGGYTCITANDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            3607..4737
                     /locus_tag="KPG09_001356"
     CDS             3607..4737
                     /locus_tag="KPG09_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0965977.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDAISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLIINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRIIEQDEISKIWSKFYRSVQSQ
                     RKGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            5142..5987
                     /locus_tag="KPG09_001357"
     CDS             5142..5987
                     /locus_tag="KPG09_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0965978.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            5987..7015
                     /locus_tag="KPG09_001358"
     CDS             5987..7015
                     /locus_tag="KPG09_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0965979.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGVKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            7018..8031
                     /locus_tag="KPG09_001359"
     CDS             7018..8031
                     /locus_tag="KPG09_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0965980.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTML
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGAPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILVVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(8513..9763)
                     /gene="cme"
                     /locus_tag="KPG09_001360"
     CDS             complement(8513..9763)
                     /gene="cme"
                     /locus_tag="KPG09_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBG0965981.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHNIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLVMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIVAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(9849..10595)
                     /locus_tag="KPG09_001361"
     CDS             complement(9849..10595)
                     /locus_tag="KPG09_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0965982.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(10710..11732)
                     /locus_tag="KPG09_001362"
     CDS             complement(10710..11732)
                     /locus_tag="KPG09_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBG0965983.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWICKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(11732..14152)
                     /locus_tag="KPG09_001363"
     CDS             complement(11732..14152)
                     /locus_tag="KPG09_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG0965984.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLALLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKILINQGNLNTFGTNLVNDILSKTRHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSDSEIGDEDDYAVIETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(14238..15158)
                     /locus_tag="KPG09_001364"
     CDS             complement(14238..15158)
                     /locus_tag="KPG09_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0965985.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHFVDIYLPIEPF"
     gene            15768..16694
                     /locus_tag="KPG09_001365"
     CDS             15768..16694
                     /locus_tag="KPG09_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG0965986.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSENS
                     NAEIVTKNSDIFKNIEKYIDATINNNQKTMKYKHRDELFKNSNTNTYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENLDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
     gene            complement(16882..17502)
                     /locus_tag="KPG09_001366"
     CDS             complement(16882..17502)
                     /locus_tag="KPG09_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0965987.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYEEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTNMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(17474..19057)
                     /locus_tag="KPG09_001367"
     CDS             complement(17474..19057)
                     /locus_tag="KPG09_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0965988.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFL
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPEVQDIIYETLNNLNTCIRNQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNINDNGVKNTSFKTPLDITKITFDIIKHKGIISIKEQVENLGGTISISDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            complement(19201..21228)
                     /locus_tag="KPG09_001368"
     CDS             complement(19201..21228)
                     /locus_tag="KPG09_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0965989.1"
                     /translation="MNIFYMAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFITGLVLGALSHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAISNGKFLYKTSLVKLIRHEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQKSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLINGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTSILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYDNIQIKKIIKKQVLPFFNIPFLLGILDCIFAT
                     LVYKTALMQNLLGNSFSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKN"
     gene            complement(21241..21990)
                     /locus_tag="KPG09_001369"
     CDS             complement(21241..21990)
                     /locus_tag="KPG09_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0965990.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFDIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYAASFANRILIFKDG
                     HIIKELNKQPNNRHTFYELIIQEIAKLDTKR"
     gene            complement(22287..23939)
                     /locus_tag="KPG09_001370"
     CDS             complement(22287..23939)
                     /locus_tag="KPG09_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBG0965991.1"
                     /translation="MNNKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYKMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSDILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKIEYEITINAITGKILEFEIDD"
     gene            24538..25659
                     /locus_tag="KPG09_001371"
     CDS             24538..25659
                     /locus_tag="KPG09_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG0965992.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            25695..26108
                     /locus_tag="KPG09_001372"
     CDS             25695..26108
                     /locus_tag="KPG09_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0965993.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            26101..26640
                     /locus_tag="KPG09_001373"
     CDS             26101..26640
                     /locus_tag="KPG09_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0965994.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEASCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVAAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(26840..27325)
                     /locus_tag="KPG09_001374"
     CDS             complement(26840..27325)
                     /locus_tag="KPG09_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0965995.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFDLDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            28176..28553
                     /locus_tag="KPG09_001375"
     CDS             28176..28553
                     /locus_tag="KPG09_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG0965996.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
BASE COUNT        10348 a         3693 c         4254 g        10346 t
ORIGIN      
        1 tatagaaagc taatctaaag aagtataata aactaattta ggaagtatag aaaattaatc
       61 taaagaagta taataaacta atttaagaaa tatagaaaat taatttaaag aaatacaata
      121 cattaatttt taagtaacat taaattaatg taagtaaaat aatgttcata tgaagattta
      181 aaggtgcatt ttctgaagct aagtatatgt tatgtctcta tatccggtga agtttaatag
      241 ctcaagaaaa tgcactattt atgatgatta ttttgtttta tttccaattt tggaaataac
      301 tattctaatt aaatctaaaa ttataccata aataagaaaa gaaagtatag atgctatgta
      361 ccaataaatc gaaattgacc ctgtaattgt tctacatggg atttgcatgc ctgaatccat
      421 aaaaacaaaa aaactatcaa tggtagaaat aatagacaaa ataggattta atcctattag
      481 aataatactt tttgagtctt gaccaatttg gtggatataa atctcaaata atgcaattaa
      541 aaaaaacaaa atgtaaaaga tttaataatt tttttcatca aaatacctca cctaatataa
      601 attctgtttg cactatttgc ttactataac tatttatctc cattcagtca catcacttaa
      661 ttgtaatcta ggtcttcttg atggttcttc gtcaggataa cctaaagata ttgctgccac
      721 taattgacta tctgtattta accattcaca taattcacga tatgcaaagt atacatcaca
      781 aatccaaaga ctacctagac ctaattccac agctgtaagc gacatattct gaattgcagc
      841 tccaattgat tgcatgtttg ccatttcata gaatcgttct tcaggggata gcttttctaa
      901 aggtgattta cctaatatat ttaaaataaa aattgttact ggagcttgtt tcatgatttc
      961 aactgtgtaa tttgcaccag cgatatgttg acgacttcct ggaagaagcc cattatcatt
     1021 aatttcattt tgaatacctt tgctcatggc tttaagcata gattcttttt ccttttcagt
     1081 gatgacaaca aaacgccatg gttgcctgtt tttagatgaa ggtgcattta ttccagcttc
     1141 aattattttc tctatagttt catgtgaaat gctttgattt ttatattttc tgatacttcg
     1201 tctttttgat atagaatcac ttatcatttt aagtaacctc cgtttatttt ttggaaaaat
     1261 ctatttattt tctattttta attattttag cattaatcag aaaaaaagtc attgatttta
     1321 gtatacaatt attaacgatt atattaagga ttgtatcaaa aagtatgtga ttatactaag
     1381 ggattatatt agacattata tttaaaattg tatgaaaggt tgtatttaat attatattaa
     1441 gagagatata tattaggatt tatatatatt aagatttatt atattgagcc cttatattaa
     1501 attaaggatt ataatttaaa aataagctat aaaaacccaa tattgttttg ttcagagata
     1561 aaaaagaata ctttaggtga tttttattaa aaattaagag tgataagatt atatacttta
     1621 taaatgtaaa tatgacgaat aaaaatgata aatataataa tctatatatt tacattagga
     1681 gttttctata ctataattat ttatatttaa aactaaatat aaatgttgga attatttagg
     1741 atttgaaatt gtctaaagat aaatataagt gatgaagagg tgatgtgatg agattagata
     1801 tggatagtat gttatatgta ctaatttata tgacattatt ggtatctatc attggaagta
     1861 tattttattt ttgtaaagac ctcttaaatc ataaaattaa gtatgtttta ataagagaca
     1921 tatttgtaaa tagaattaca tattctgaag aacggttgcc taaacagtat atagtttttc
     1981 agaaatatga tatttggagg tattgtagtt tgttttaatt tgaaatgaat ctattataaa
     2041 ttctaaagtt taaaaataaa aaggagagaa taaaatggat ttcatttcaa gtatattgca
     2101 aggattatta agtatattat ttaattttac aggtgatttt ggaatttcta ttgtacttat
     2161 aactttaata gttaaattag tacttatgcc actatcatta aaacaaagat tttctgtaaa
     2221 aaaacagcaa gaattagctg aaaaaatgga acatataaaa gaaaagtata agaatgatac
     2281 taaagaattg gaaaagcaaa tacaagtaag ttctgtagaa agtatgaaga gtatgctggg
     2341 atgtcttata gttttggttc aaatgccagt agtatatgct ttatatcatg tatgtttaga
     2401 tatgacaaag gaatttacaa caattataat tccatggatt gctaatttag gtacatttga
     2461 taatttgttt atattacctt gtatatatac attaattatg ttagcttcaa atttagtgaa
     2521 ttatatacca tatttaaaaa taaattctca agtaaagttt aacaaacaaa tggctgtggt
     2581 gactaccata acaagtctta tactaactgt tagaactcca gtagccatag gtctgtactt
     2641 tataacaagt gcaatttatt cattaattga agatatttgt tttagaatgt attttaatcg
     2701 aaaaaataga atagtgacaa aataatataa ttattattag aaatatttta gcctgctaac
     2761 atagaaaaag ttagtaggct atttttatct atgatatcaa ttccattaag aatagtctaa
     2821 aaaatactat caaaatgcta taaaaaatac tataaaaata ctataaaaaa atagttgaaa
     2881 aataaataaa ttaatataaa ctaaaataaa gcctacaaaa aagaggtgat aaatagaaat
     2941 ggctaaaata ctagttgtag aagatgaaaa aaggatgcaa agtattattg tagaatatat
     3001 gcaaaaggga ggatatactt gtataactgc taatgatgga gtcgaggcac ttacaatatt
     3061 aaagagcaac aatatagatt taatgatatt ggatattatg atgccgtatt tagatggatt
     3121 ttcagtgtgt agagtttcac gtgaaatgac taatatacct attatcatat tgacagctaa
     3181 aggtgaagag gaggacaaat taaagggtta tgaatatggc gcagatgatt atataacaaa
     3241 accatttagc ccaaaagtac ttttagcaaa agttaatgcc ttgttacgta gatacactac
     3301 tgatattccc aaaaattcat tgagtttagg aaagattttt attatggtag catcaaggca
     3361 agtgtatgta gaagataaat tgattgattt aacatataaa gaatttgaat tattgcgttt
     3421 atttatggaa aaccctaatc aagttttttc cagagagaag cttttaaatt gtatatgggg
     3481 atatgatttt gaaggaaaca cacgaactgt agatacacat ataaaaacct tacgcaaaaa
     3541 gcttggtagt gaaggacacc atatagttac attaattcgt tcaggatata agtttgaggt
     3601 taaggaatga atagtttaag aaagattata gaaaaactga aaaatgtaga tatttcaaat
     3661 tggattaaac aaaattttgg actatattca gttagaaaaa aagtgttttt actttctaaa
     3721 ttatcaggag cgttaattat attattttat ctggttgttg aagaattttc gataaactct
     3781 aaatttgaat tttggatttg gtttataata ctggttatat ttattatgag tattgatttt
     3841 ttattgggaa aatttatttc agaaccaatc acatctataa ataaatctgc aaaaagtatg
     3901 tcccaacttg atttttctaa tccatgtact gtaaatacta atgatgaatt tggagaactt
     3961 tctaggagcc tgaatacaat gtctacaaat ctacaacagg cattatcaga cctagaaagt
     4021 gcaaatattc aacttgaaaa ggatgtaaat aaggaaagaa tgctattgga acaaagaaag
     4081 gagttggtag atgcaatttc acatgaaatg aagactccac ttggcattat acgtgcgtat
     4141 acagaggggc ttatggatga agttgatgaa gaaaaaaaga aaaattatat gaatgttata
     4201 atagaagaaa ctgatagaat gaacaatatg attgtatcat tattagactt atctgcattg
     4261 gaagcaggag tttctaaact taacccagag cgttttgatt ttatagagtt tgttgaaact
     4321 gtggcaggtc gtcttttaat tgatatacca gatgccaatt tttattttac atatgatttg
     4381 ccagagtatg aagtttttgt agtggcagat aaaatgagaa tggaacaagt agtagagaat
     4441 ctaattatta atgcaaagaa gcatgttgta tataatggta atttagattt atcgattaca
     4501 tgtgaaaatg gcttgctttt atttgaaatt tataatgatg gtaggattat agagcaagat
     4561 gagatttcaa agatttggtc taagttttat cgtagtgttc aatctcagcg taaaggaggt
     4621 tcaggtctag ggcttgcaat tgtttcacaa atacttacaa tgcaagggtt agaatatgga
     4681 gtgaataatc gtgatagagg agtagagttt tattttatga ttccaattga tgaataattt
     4741 agtattaatt tataaattat aagtatctga tgtaaaatat ttttctttgt atacgtaagt
     4801 atttaaagag aataaaataa aaaacttaat gaaaaagtct aataaaaagt ttaatgaaaa
     4861 gtcttgtttt cctgatgaaa acaagatttt ttattaagct ttttattatt tcacttcgat
     4921 ttcacaagag agacactctg atttcaccaa atagaaataa aataggttaa ataaattaaa
     4981 aaatagaagc gtaattaaat aaggttacaa gggggagtac ttatgaaatg gtatacatgg
     5041 ttgactatta tattgataat attgctttct attccgctta aaattaaatt tataaaatgg
     5101 tgggatagac gacaaaaaaa tgataaagga ggttatgata gatgataaaa gtaggagagt
     5161 taaaattttc ttatggaaaa gataaacaga tattgcatgg acttaatttt gaagtaaaag
     5221 aaggagaaat ctttggattc cttggaccta atggttcagg aaaatccaca acacagaaaa
     5281 tcttaaatgg cgttttaaaa ggttacaatg ggcacgtatc cctttttggg aaggaagtta
     5341 aagcatatac agagtctcta tatcaaaaga ttggtgtttt atttgaattt ccttatttat
     5401 ataccaattt aagtgcaatt gataatttag aatatttttc gtctttttat cctaaaaatc
     5461 aaaggagaga catatatgaa ctcttagatt tgttagagtt taagaaagag tttataaata
     5521 agcctgtatc atcctactca aaaggtatga agcaacgtat tagcatggca agagcattgg
     5581 taagtaatcc aaagttgtta tttttagatg agcctactag tggacttgac ccatctggag
     5641 ctgtactatt tcgtaagatt attgaagaag aacgaaaaaa aggaacaact atctttttga
     5701 ctactcataa tatgctagat gcagatttaa tgtgtaacaa tgtggctttt attgcagatg
     5761 gcaagattat ggtaatagat aaacccaaaa atttaaaaat gaaaaatagc aataataaag
     5821 ttgaagtgga atttgtatat aatggaaatc gagatattga aattgtagat atacaagagt
     5881 tagaatctgg aataactttt aaatatgatg aaatcttaag tgtacattca aaagaaccta
     5941 cactagaaga agtttttatt aaatacacag gaaggatgtt agtgtaatgt attctaaatt
     6001 ttttacctta gtaaaaaaag atttttggat gatgatttat ggtaaatttt ttgtgctgac
     6061 acttggctct ctcatacttt attcgtgtta tattaatttt ggttatgtaa agtttatgga
     6121 tgaagatagt tatcacaata atgtatatct atataatcca gagaatataa gcatgagtgc
     6181 atctccatta attaatcaag tttcttctat aggtgaatta aaagcaaaac ttgatgaaga
     6241 tacaagtgca gtttgtattg atatgagttc cagtaagccg aaagtaattt tacatgaaag
     6301 tattaaagga gttgacaatt atagagctga ctatgcattg tcattattat catcagaaaa
     6361 agaccataat agcaaaatat cagacaacaa tgcaataatt caaattaatg ataaggatac
     6421 atctgagata aaacaaagaa aagacatgac ttgtgagatt ttattctttg aaattgtagc
     6481 tattggattt ttagggattg cctctttatt atttaaagaa aagcagatgg gagtaatgcg
     6541 tattactggt attttacctg taaaaagaag tctttttata ttatcaaaga tatttgcttt
     6601 tttgatatgt gacctatgtt ttacagttct tttagtagta tttaatattg gattttttga
     6661 aggagtaaaa atacttccac aagtattagt tcaagctagt attttatcaa ttattatgtc
     6721 tttgattgga ataggatgct caatgttatt gagagatttt aagcaatttt ctcttatata
     6781 tcttgtggtt gctgtattca taactacacc tatatttcta tctgcaaata catcttttga
     6841 aatagattgg attaaatatc atcccttcta tcatgtttat agtcagctta aaaatgcgtt
     6901 tttcatgaat ttttcaaaag aattagttta ttttgtagtg tgttctttga caataatagt
     6961 tctattttta cttgtaaaat atatttttaa taaagagttg gttagggagg gataatcttg
     7021 ataggattac gttaccagtt aaagagtata aaaaaagata aaatgtgtat aatttccttc
     7081 tttttaccag ttgttatggc gattttaata aattttatgg gaacaattga tatatcttca
     7141 ttaggagaga tacgttttgg tatagtgaag gatacacttc ctgtacaaac agagcagtgg
     7201 ttgaagaagt atggtatggt aagtgtatat gacacaaaaa tgcatttaat acgtgaaatt
     7261 aaggatatta aaacggacac aattggagtt gttagagatg gtaaaggaat aaaaactatg
     7321 ctttatggaa atgagattaa tgctataaaa aatgttggaa agacattgcc agagatgttt
     7381 agactaaagg gtgatttaaa agatgttgag gttacaatat tgcctaaaga tagtatttta
     7441 aaagagtttc agaatgtttt tattgctatg attttaatta ctgctatgtt tatgggatgt
     7501 acctttaatg caatgaatat tatatctgaa aaagaagatg gaatatcatt tataaatgaa
     7561 gttttgccac agagtagaag tcagtatatt atacaaaaag tatttttggg tcttttatgt
     7621 gggtgtttat cttcaatgat aactgtattt atatgtttgg atatttcaat attagatatg
     7681 ataatgatgt tgttactcgt tgtattttca gcatttattt catcacttat aggtttattt
     7741 attggaaagt tgtcagaagg tcttatggta ggaattgttt atattaaaat agtaatgatt
     7801 ttatttatag gtgctccttt gttaatatat cttctagggg ttaataccag cggtataggg
     7861 aatttatgct atataatgcc atcagttgct acatttaatg gtattatgga gattattaat
     7921 ggtaatgtta ttattataaa agatattgta attttggttg tacattgtgt agtatggttt
     7981 atgatatata ttattttaga taaaaaaaga aagttatctt ttagtagata actcagtata
     8041 attataattg ggacaattta ataggtgatt taatatactt tgtatagtgt aatttatgta
     8101 gtgcaatttg tatactttaa tttatgtagt gtaatagtta gttttatttt ataattattc
     8161 aatataattt agtaagaaat tcagtacaat tagagctact tagtggaaaa cttaataggt
     8221 acttaaacta tataaatact ttattttcct ccatttgtac tataaattta atttatgata
     8281 taaatggagg tattattata tgaggtatta ttatataaag ttaataaaga tagtatcaaa
     8341 atattatttg ctataaagag agggactcaa aataaagata aacctatttt tcaatgttga
     8401 aatgataaaa tatatttgat ttcatataaa aaattttctt cataaattaa aaagtccatt
     8461 ttaagccccc tcatttttaa ccagataaat cgagttaaac tatttaaata tattactcgg
     8521 attcatttga cttagatatt ccttgcttgt aaaattgttt tgaatagggg atagccaaag
     8581 caaaaaataa aagtataatc cctgctacga ttattaaatt attaatagaa attacatctg
     8641 ccattggtcc gaaaatcatc ataccaagag gaataaaacc agtatatata ataataggta
     8701 agctaagaac tcgacctagc atttgtggtg ctacatgctc ttgcaacata gtcatagctg
     8761 atgcttgaat aattggaatt gaaaaattcg taaaaaacat aaatataaaa aattgccaag
     8821 cttgtgttac aagacctaga ataattgaaa aaatagcata ggataatatt gctagaaaaa
     8881 aggtcttatt tctattttta aaaccaccaa aaactcctat aatcaatcca cctaaaacca
     8941 taactaaaaa tccaatcatt tcaataatag acaggtatat ataacccttg ccaaatgttc
     9001 gttcaataag aagtcctata agaaatcctg atggaacaca aagaagcatg aatgctccat
     9061 aagtgaaaag cacttttcta atgaacttat ctgaaaaaca gtactttatc cctgatacca
     9121 tatctgcaaa aaatgagact ttttcagttt gttttggtat attatgttta ggaattttga
     9181 tgaatatcaa taatacaata gcaataattg cagtaaatac atcaattaaa agaacttgat
     9241 ttattttatt accaaatcca agtattgcag cagctacaat aggcactata aattgtacta
     9301 tagactgtag actactattt ataccattaa aacgcattaa atgttcttct ggtacaagtt
     9361 gaggaatcat tgcgtttaca gcaggtgttt gtacaccact tcctagtgaa cgtataacag
     9421 atattactga tatggctatt aattcacttg ttgtgccatt tgatgacatc ataaatagaa
     9481 ttagaactaa tgtagatata gcaattgttg aatctgatac aattatgagt acctttcgat
     9541 tgtagtagtc tgcccataca ccagcaaaaa aagaaattat catttgaggt ataaaggagc
     9601 atacagttaa aactgttacc catacacctg atgatgtctg aagtgtgaca taccatatca
     9661 ttgccatttg tacaagtgat gagccaaata aggaaatata ttgacttgct aaaaaaagaa
     9721 tgactttttt cttccatgta gaattagtat ttttgttttc catcctattc ctcctttgat
     9781 ttgtgtgttg aatttttctt gctcttaagg ataacacaca aatcaaagtc tggagttaga
     9841 gtggagggct aggaaaattg ttttttcata atttgaagat gagatttcat ggtttttgca
     9901 ttatgttcag cttctttcaa tgaagttatc agtttgtcac acactgagat aatttcgtca
     9961 tcatcttttg gagtgtcaag gacagtttta atattagtat taggattaga agataaagca
    10021 tttaacattc gtaaaatagc tgaaagagaa tagtttgcac aacgaagtgc gcttataatt
    10081 ttaagtcgtt ttatatcttc atcagtatat acacgataac cattttgttt tctttttact
    10141 tttaacaaac cattcatttc ccaatttctt aaagtatcta ttgtaacatt taagtgttct
    10201 gcagtttctt ttctagttaa aaagagttta tttgtttctg gagtatttcc atttaaaatc
    10261 tgtgcaacaa tagtaagtgc ttcctcggca ttagattttt ctatttctaa ttgttgcaag
    10321 tattgattta caagactaat agccttatca aagtccctat tcgctgatgt tcttacaatc
    10381 tcaagggcat cttttcttaa tcccctttgt accacttcaa ttttcaaggc tgtacgagca
    10441 aatctaaatt gttctatatg aaaatcatta aagatacgat aaccattggt attacgctta
    10501 ggtttaggta ttaagtttag ttcttcatat aaacgtactg tattaggatg tacaccaatt
    10561 aaatctgcta cctctgatgt cgtgtaagtg tccattagta ttcaactcct tttagatata
    10621 tagtattagt cataataaca ccaaacaaaa gtctggtgtt atagtggagg gtttggttat
    10681 ctaagagtaa ttagtttatc taagagtgat taatctatta tagaagtaag tatatatttt
    10741 tcaataaact cccatctatc acgaggttta aacactattt tagatggtat ggctattact
    10801 aagtcatatt gtggaataca acaaatagca tttcctccag ttcccattgc tgaataccac
    10861 aaaacaccat cttttttaca tatccaccac agataaccgt attcattact attcatttta
    10921 accgattctt caatccacga tttagatata atacgtttat tattccaaat accatcattt
    10981 aaatacagaa aaccaaatcg caacatatct ctagcactca atgtaagtcc ccagccacct
    11041 atagaataac cctgtctatc ttttgcccaa cccttaagat tttttccaaa gaaatcttca
    11101 aaaccaaaat ctttcatatc ataatctggt ataactttca taccaatagg tttaaataag
    11161 tactcattag caaattcacg agcagatttt ccagtagttt ttgttataat agctgaaagt
    11221 aggtgcgttc cagctgaaga atatttgaaa gtaccaattt cgcctttttg accaagttga
    11281 ttcaaggtat attttatcca atctttttgt ttgcaaagtc tttctaaaga ctcagtcata
    11341 tttttaaaag ggtatggtgc agtcattgta agtaggtgac gtatagttac tgctttttta
    11401 catatttcag aaggactaca agtatattca gggaaaaaat caacaacctt ttggtcaaca
    11461 tcttttatat atcctttatc tattgcaata cctattaagg ctgatataat actttttgta
    11521 actgatgcaa catgaaaagt atcatcagca ttatatccat tatagtactt ttcaaagaca
    11581 acttttcctt ttcttgcaat aattattcca tttatattac tatattgtga gattattaaa
    11641 ggttccagtt gtgagagctt ttctgaactc atgccaacac ttgaagggtc tactttttca
    11701 aatattaaat ctaagcaatt gtttttattc attaaagata cctccctgtt atagattttg
    11761 gattttcttt tagagttttt ggagaacctt ctgcaattat ataacctcct ttgtcaccac
    11821 cttctggacc aagctcaaca atccagtcac aaacatttaa tacatctata tcatgttcaa
    11881 caacaattac agaatttcca ttttccacca attcatctaa taattgaatc aacttagaag
    11941 tatcatatag acttagacca gtagttggct catccaatac ataaaggata ttaccatttc
    12001 gttttttacc aatttctttt gcaagtttga ttctttggga ttcaccacca cttaaagtcg
    12061 gtgtaggttg gcctaatttt atatacccca tacctatttg ttctaataca tttaagtgtg
    12121 aaacaatatt tggaatatcc ttgaagaaat gtacagattc agaaacactc atatttaaaa
    12181 tatcatgtat atttttattt ttatatttaa tagataaaga ttcatcatta aatctttttc
    12241 cctgacattc actgcatata tggtctatac ttagattatt tcctaaccag accttttcat
    12301 atccactacc actacattta ggacaagcac ctttggagtt aaatgagaaa tgaccagctg
    12361 taaatcctcg ttctaacgat tctggctgac ctgcaaatag tgtacgtatc ttatcccata
    12421 taccaatata agaagcagga gttgagttca tatttttgcc aataggagat tgagatatct
    12481 ctgaatatcc agaaatatgt ttagtaccaa tcagtctatc tgcaatagtt tcaataactg
    12541 cataatcgtc ttcatcacct atttcgctat ctgaagaatt tatttgatta ttgatagtat
    12601 ggttattaaa ttggcttcta agaagaggaa gtagggtgtc tgatataagt gaactcttgc
    12661 cactacctga ttttccagat attccaacca tagcacccaa aggtaatgat acagttacat
    12721 ttttaagatt atttgtattt gcattttgaa tagttataca aggtatttta tcagtatgtg
    12781 taatattttt attgcttgtt ctattaggca ttggatattt tccagataga tactgcccag
    12841 taagtgattt gtcggattga agtagacctt ctaaatctcc ttgatatact aattgaccac
    12901 cttcaacacc agcttttgga ccaatatcaa taatatgttc tgccatttca attgtgtttt
    12961 tatcgtgctc aactactatg accgtattgc ctaaatcttt aagtttcttt attgatttta
    13021 agatgtctat tttttctgat tcatgcaatc cagctgttgg ttcatcaagc acataaatta
    13081 atgagtccat ctctgaatcg agatgtgaat ttaaaaatag tctttgcatt tccccaccac
    13141 tcaaagttga catttctcgg tataaggata gatgacctaa tcgtgattct attaaatgtc
    13201 gagttttgga taaaatatca tttacaagat ttgtaccaaa tgtgtttaaa ttaccttggt
    13261 ttattaagat tttaaggaaa tcatcaattt ctaaaatcgt catttttcca agttctccaa
    13321 tatgttttcc gtttaaaagt actctacgtg cttcttcacc aattttaaaa ccattgcaat
    13381 ctgaacaggc agtcatagta taaactccat ttaaatcctc accccttttg taacgattgt
    13441 gaaatacctt tgataaagag gaactttttt taccattttc ataatgacca tagagtactt
    13501 catttcttat ttcttcaggt agttgtaaga attgtatgtc taaataatcg ttgaattttt
    13561 tagaaaatac tctcaaatat cctggtgtaa tctttgctct atctaagact tgttttaggg
    13621 tatcatgctt atcaaggact aatttttcaa tattaatctc aaaatatgca ccatgtccag
    13681 agcacttcat acacattcca tctgaagagt tgtatgagaa ataacttggt cttaatcgtt
    13741 cttcctcata cccacaatta ttgcaaatta gattatcgct tacaggagta tcacacatag
    13801 tacacattat ttgaccttct actgaataca atagtgcaag catgttgaga atattggttc
    13861 ttgtgcctac tgttgaacga ggattgctct ggcgaataat attttgttga acagctatag
    13921 tgggagctaa accttgaata ttatcaaact tatcctcact atcaatacct gcaagtatac
    13981 ctaaagattg cagatattgt ttacgacctt cctcaaatac aatgtcaaac ataaggctag
    14041 acttacctga gccactgaca cctgttgcaa caacaagctt atctttgggg atagaaacat
    14101 ctatattttt aagattgtgt atctttgctc cattaatttt tatttctttc attggttcac
    14161 ctcattttat caattttatc aaaccgtaaa atagggatat attaatcctt aatacaagaa
    14221 tagattaggt gaggttatta aaaaggttct ataggtaaat atatatcgac aaagtgtcta
    14281 ttttgagggt gattgtgtgg gttgttaaga tatacttcaa aagggaagga ggcacgtagc
    14341 ttataattac catttgatag ccattcatta tacataaaat cccacgcatc actatattca
    14401 gattttgaaa tatcaaaatg tcccacaaga tattttccag aagggattat catattgcca
    14461 agattgtcat tctctttgac aacaatgtta tctggaatgg tcatgcaaat actagtcctt
    14521 aattgatgct cctgtgtaaa ttgtggatta tcatgataaa tggctaatat ttgtgtgttt
    14581 tcaagtccaa gtagattctg ctcacttgca tatgtgaata accgttctaa aagttttggg
    14641 aaagtactgg ttagattacc atatgaacca gtatatctag tgtaaatagt attcatatta
    14701 tcaagttcta gaatttcaat ttcaccttta acctctctag tattcttaca tttaggtttt
    14761 gatatgcttc tattgtactg agaaatttta tatggttctt tgcaattctt gctataattg
    14821 tttctatatt ttatagggct tattttatag taatttttaa atgctctcga gaaaactgct
    14881 gaatcagtaa agccgaagta gtgtgcaata tcagtaacag tcatgtcagt tcgatgaata
    14941 agaaaagcag ttgcattttc tagttttagc ctatttacat attgtgccaa agtttctttt
    15001 tctattgctt tgaagattct atgaaaatga aattttgaaa aacctgctat attagaaagt
    15061 tcatcaagag aaagaggttc atgtaaatgt tcttctatat agtcttgcac tttgcaaata
    15121 cgatttagat attctttttt acttttagca tttgtcatat atagccctcc ttattgtgta
    15181 aaatgtgtat taattaattt atagcaatat agtaataaaa ttttatgttt ttgcaattta
    15241 tgataaataa gatagcgttt aattttttat aattgataaa agatatttat aattaataaa
    15301 agatataaga tgccttatat ttatttttgc atataagtat agtcttcgta ataattagta
    15361 taccatagtg atttattttt aattgtgtat ttatataaat acacaactta tgatatggac
    15421 gaattattat gcaaataagc tgtaattaaa taaattgcaa tataaaccag ttgaaacata
    15481 agttagggaa atattatcaa agttattatt tagcataaga gtatagaggg aataaaataa
    15541 gattaaaaaa ttacatacta aatttaataa aattattata ttaaaaataa tattaagttt
    15601 attgtttaaa taaaaaagtt aaatctacac tattaactta ttatacttaa aactttaaaa
    15661 tgaaaatatc aaagaatact ttttaataaa aataatcatt taataagtta tttaaaaatc
    15721 taaaaacttt acaacaaatt atctatttaa attggaggag tgggaatatg agtaatagtt
    15781 tttatcaatt aaattcagca gtattatatt ttaatgaaaa aagagatgta aatagaataa
    15841 ggtttaatca ctatgtaatg agaaaagaaa atatacaaag attaaaagga actctgccaa
    15901 gaggacaatt tatgatgaca gtaaggaaag ctgcatctga ccttgatgta tctatatcta
    15961 caataagtag acttgtaaat gaatttatag atttaggtat attgagatta ataagtaggg
    16021 gagtaaaggg aaattgttgt tctgtatata gttatgtttg ttctgaaaat agtaatgcag
    16081 aaattgtaac aaagaatagt gatattttca aaaatataga gaaatacatt gatgcaacta
    16141 ttaacaataa ccaaaaaaca atgaaatata aacatagaga tgagttgttc aagaacagta
    16201 ataccaacac atacagccgt ataaatcaag agagtataga caaagattat gatttgataa
    16261 ttcatactgg gtatgaaaca gaaaatgaga caaagaaaaa agaattatta aaaaataatt
    16321 taaataaaaa gaatttaaat aaaaatacct ttgaaaattt ggatgaagat ttgactgaag
    16381 attctactta taaaaatcca gaaagcatac atgaaattat tataagaaaa ttaaatagtg
    16441 aatcaggaaa aaattttaaa gctgatttga gtataactaa ggcacttata gataaaagat
    16501 taaaagaagg gtatagttta gaagattttt acaaggttat agaggcaaag gtaagaagat
    16561 ggagatatac agatatggaa atatatatag aaccaacaat tctgtttggg tatgaatttg
    16621 agtcgtattt aaatgaggag agttctttat ctgcttgtaa ttatggtgat atgtgttatg
    16681 aagatatgta ttgagatatt agttgctttt tagttgttta aaatatgttt atatataaat
    16741 ataaatagtg tattaaaatg gagttgtctc aaaatagaga gaagcctctt cttaattgtt
    16801 taaatgatga aaatatattt gatttaatac aaaggagagt gccttatgag ctagaaagtt
    16861 cattttgaga cactcacttt tttaatatac tggagaaata taacccattt taatggcttt
    16921 tgtaatagcc tcaactgatg atgaaacatc tagcttgtca tatatgtgtt gtaaatgatt
    16981 tgataatgtt ctttcactta tatacaattc atcagcaatt atttttcttt ttttaccact
    17041 acagataagc tgtaaaattc ttttctccat attagttaaa tcttcaataa ttacttcagt
    17101 attttcagat ggaaaacagg ttaccccttg atagactttt aataatgaat caattaaact
    17161 atcagggcta atattcttat ttaggaatcc atagatacct atttttttag cctcatgttt
    17221 ataaactggg aggtcgtatc cagtcaagat tattatttta atagttgaat tagtctctaa
    17281 taaattataa gctactttta agccatcaca ttcacttaag cctctaaggt ttatatccat
    17341 taagacaatg tctatttttt tatcttgaat catttctgaa agattatgga tgtcctgtgt
    17401 ggaataaaac tgttcgattt cttcgtattc ttctagtgca attgacaagc ttttagcaaa
    17461 aagcatatgg tcatcaacta acaaaatatt tataagaacc atctcctttc atagggattg
    17521 taatctgtac acaaactcca tgaggtatat tatcagaaat acttatcgtt cctcctagat
    17581 tttctacttg ttctttaatt gatataatcc ctttgtgttt tattatatca aaagtaattt
    17641 tagttatatc taaaggtgtt ttaaaagatg tatttttcac accattatca ttaatattta
    17701 attttattat atttttttct agtgctaaag ctatccatgc atggctacca tttgaatgtt
    17761 tatacacgtt tgtcaataat tctttaatca gtcgatatat caatatatca tatggttcaa
    17821 ctaagaataa agtatcagaa cacttaaatg aaacacaaat gtttttttgt ggaaaggatg
    17881 aagaaacact ttcaattaaa ttctgataat tttcttttac tgttaaattt tttaaaataa
    17941 caggatgata gtcttgcatt tgatttctta tacatgtatt tagattgtta agtgtttcat
    18001 atataatatc ttgaacttct gggcgatatg acttactcat catgtttttt attgaaagca
    18061 ggttctgtaa tatgtcatca tgtaaaaaat tagaaaactc tgtttttagt tgttcttctt
    18121 gttgtaattt ttctaatgca aaagtatact tgctattttt aactacttta tttttttcat
    18181 ctctaaggtt aaactccaaa ataatgttac agaggaaaat aagcataaat aaaacattta
    18241 tagctattaa tattatagaa aaattcccat tggcaaatag tacgaccaag ctaattacta
    18301 aaatacttat aaaaagaaat atagcccttt gttttatgtt aaaaactgtt gaaataggaa
    18361 aactatcatt ttctttaagt gcaataccat gaatactcat aaaaaatacc aatacagtta
    18421 gatagattcc aaaattacca atatgatttg atattcttag tgtaaaaaaa taataagtaa
    18481 ggaatcctat aattgttata aatactgaaa tcgatatagg ctttttttcg ctttttatga
    18541 caaaaacaac ccttttcaaa tgataaaaaa taatatataa aaagcttatt atactaacaa
    18601 caaactgtat tccgtacata caagcatacg ttctgttaga aataaatgct cctacaattg
    18661 caccaatgca ggtgataagc aataaaatat ctgttatctt acggaaattg tatccactac
    18721 cttgaaacaa aaataaaaat ataaatttaa tagagacata cactataact gtgcttaata
    18781 ttttaaaaat aatagtgcct attggtgtgg ttacaaatga taagagtaaa taccaactgt
    18841 ctagtgctag taatccacaa aataatgaaa taatgttatt ttctcggaca ttacaagagg
    18901 ttatatatgt gatgtctatg agtaataatg ttgaaattat taagggaata atgctttgga
    18961 tattcatata agtttcagtg cttgtagata ttcccatgaa gagaagtgat agaatgtagt
    19021 ttcctaaaaa tagtattttt aaattaaaat ttgtcaatct tatacacctc cttatgagtt
    19081 aattatatta attttatgac taaatcaaac ctaagaatct ataattttaa attatatcaa
    19141 atacttataa atgaagtata ccaaatcgtc acacctctaa gcttgacgat ttggtaaata
    19201 ttaatttttt aatacaattt tacaacagga tttaatagta agtatataat atatcaaata
    19261 cactacagaa gttaagaaaa ttgcaataat cacaggtatg tattgtgaaa atgaatttcc
    19321 aagtaaattc tgcatcaaag cagtcttata aaccaaggta gcaaatatac agtccaatat
    19381 accaagtaaa aacggaatat taaaaaatgg caatacttgt tttttaataa tttttttaat
    19441 ttgtatatta tcataaccca ttttctgtaa aatcttataa tcagatgttg aatctgaaat
    19501 tgctgatata ttgttaaagt ataaaatact tccaacagca ataaagaata ataccacaag
    19561 aaatccgatt aacagaaatg tagaactatt aattgataat aaatcattta tcctgtgaga
    19621 atatccttgt aaataagggc ttccatctaa ataatctgaa atattactat ataaattttc
    19681 attattttta attgatttac cattaatact taaaatgctt gtcattggta atttattttg
    19741 tgctatttgc tcatatatac tatcattaac gataagtgtt ccaacgctat tggcaaaaga
    19801 aatagcattg tttaatgatg tttcccttac agctacaggt gtttctttac cattaataag
    19861 taatggataa atcgttccat tttcactata tccatcatta tttggttgat atttcattaa
    19921 aatacattca ttatccctta aaggggtaag ttgttttagt agctttgttc gattttgtgc
    19981 ctttaataaa gtataataat cagaaaaaga tatacattca aatcctgatt ctcttgaaat
    20041 aacttcttta gaagaactac tattagataa attattctta gatgtaccaa tttcatattc
    20101 aaatggaagg tcttttgatg aagattttac cttataaata tttgtctgtg taaaaataat
    20161 atcatctgta ttagacgtat attgactgac aatttgtttt acatcttcta tttgtttatt
    20221 tttttctact ctaaattcta tctctgaagg tgcaatacgc gaaacagatg caatagggta
    20281 gtataaggac agtgccataa cacttgaaac tgtcaaagta gtagcagaga gtaatgtaag
    20341 cataattaga gttttagagt ttgaacgtat acgatagata aagtttggaa tagctattat
    20401 cttcatatct gtgtagaaag tacgtttatt ttttttactc ttttgcataa tatatggaag
    20461 aaaggaattg ataaagagaa ctgtaccaat tacaatgata atcatagtta tcaagcccat
    20521 ttgataaaat ccaatactta cccatactga tttagctcca cgaagaatat ctagtgataa
    20581 acaataccca cttaaaatca taataaatcc cataaaggct ggaatttttc tgaactttaa
    20641 ttttttttct gcactttttt catgtcttat caattttaca agtgaagtct tataaagaaa
    20701 tttaccattt gatatagcta agactaaaac aactaatgct ataaagataa ttgttttgtt
    20761 tattgcattt atattaaaaa atggtatctg tgaattatca attgataaat taagtagtgc
    20821 tgtaattcca aagacaatac ccttatgtga caatgctcct aataccaatc cagtaataaa
    20881 agctccacta caaattaata cattttcaaa ggtaagtagc gataatattg taaacttacg
    20941 atagccaagt aaagcatata tgcctaattc ttttgtacga cgtctcaaga agaatcgatt
    21001 tgagtatgcc atatagaaga tgacaaatgc catcaaaaat atcgaaataa cattacacat
    21061 agattcaact ctaccatcag ttgaaatttt ttctagcatt atcttattaa cagagaatga
    21121 agtaaatgcg aaaaatacag taataacaaa agagactgaa agtagatata gagaatagaa
    21181 tgaaaagttt tttttaagat tctttattgc catataaaaa atgttcatta aattatcctc
    21241 ctatctttta gtatccaatt ttgcaatttc ttgaataatc aactcataaa atgtatgtct
    21301 attattcggt tgtttattta attctttaat gatatgacca tctttaaaaa tcaaaattct
    21361 atttgcaaaa cttgctgcat aggcatcatg tgttaccatt aaaattgttg ttttcaactc
    21421 ctcatttgct tcctgtagtg agtggagtag ttcagtagct gaattagaat ccaaagctcc
    21481 tgttggttca tctgcaaaga gaatactagg atgttttact attgctctaa ctgctgaagc
    21541 tctctgcctt tgcccaccag ataattcatt tgggtattta tttaattgag aagctatgtc
    21601 aaatcgttca gctagactct ttaccatgtt ttctatttta tctggattga gtttctgaag
    21661 tgtaagaggt actgaaatat tctcgaaaat tgtaagacta tctagtaaaa aatactcttg
    21721 aaagataaag cctaaattat ccttacgaaa atcagcagca caagtgtcat taagcgattt
    21781 caaatttata ttatttatga aaatatctcc acttgttggt ttatcaattg tagataatac
    21841 attaagcaga gttgttttcc cagagccaga agctcccatt atacctataa attcaccttc
    21901 tgatacatta aaggatacct tatctaaact cggctgttgc cctttattat aagttttagt
    21961 tatgttattt acttttaata ttgtattcat tgtatatgtc tcctttcgtc ttaatatagt
    22021 caaattatat attaacctta ttgtttcttc atgagtaaat cacgcaataa ttctacacaa
    22081 attacttata tcacaatata ttgtttaaaa tctaacttta ttaggtttgg gatgatggac
    22141 aagattttaa tttaaaagag tgcatagata tataatggca taatgtagat ttaaattaaa
    22201 aaaaataaaa taaagccata aatattgaaa aattcaatat ttatggcttt attgcttgta
    22261 ataattaata gaatatatat tttttattaa tcatctattt caaattctaa tattttacca
    22321 gtaattgcat taattgttat ctcatattca atttttcctt ttattatttc accttcatat
    22381 tctttgtcat cttcatcata cttaaatttt actattttcc caccaggcac tttatctagc
    22441 attattgatt tagctttttc agcactaata atagaactgt tgtcattagt attagagtta
    22501 ttcttatcat tatcatcaaa gtcatcatca gaatcattgt ccatgtcaat atctacaatt
    22561 cttccatcaa atgcacttat ctctaattca tatgttttct tattttttat tactttagcc
    22621 tcatattcgt tatcttcagc atcatattta caatagatta aattaccatt aggaacagca
    22681 tctaatgcta tagacttagc tttatccaat gatatatttt gtgtacctaa attattatca
    22741 tttaagtcat catctttttc aatttctatt attttaccag agtaggcatc aatttctatc
    22801 tcatatagga tattatcctt tataactttg cagtcatatt cattatcatc ttcatcataa
    22861 taacaaacta aaatatcgct tcctggaaga gcttgtttta atatttcttt agcctgtatt
    22921 aaagatattt ttttagtttt atttcttatg ttcacatttt ttagttttaa aatatcttca
    22981 aatgcttttt gattatatcc tcctcctact tgtgttattt catctatatc tttattttta
    23041 ataaattcct tttgactatt tacaaggcta ccattagata tcattatagg agaattttct
    23101 ttagcagcta atactcctat tgataatgaa tctactaatg cattttcttg gttaatacca
    23161 tctttagcta caaatacatt atttattttt ttatctttat aaaatttatc aataattttt
    23221 gcattagtat tgtttctatc tgaaccactg attctttctc tgtttggata tttctttact
    23281 gtattgtctg ataaaatctt atttcctcca actatgtaag atttttttat agacctattg
    23341 tttaagaaat ttatactact atcattgctg ttttcatctg atactaatat tataggcata
    23401 ttatgtattg ctgctggaga agctatacta actgcatcag ataatccctt tagtccattt
    23461 acaactgcaa cattttctac cttttttatc ttatctattt ctttaagtac atttgtagac
    23521 attttatata tattgtttcc tactatttta tttattttta tgttaagtga ttttatttca
    23581 ttttctactt tagaagaaat gctatttcca ctatctacaa tgtacacatt atctacacca
    23641 agccttttaa gctcagcctt agttgaattt tcaatactgt tgttgtttac taaaagtata
    23701 ggtgcattct ttagcttagc aaatggattt gcacaaagcg cactttgaat agaattacca
    23761 ttaactataa tggctgtttt tgattgaacc catcccaatt tacttacttc tactgctgtt
    23821 tcatatcttg ttttacctat taatctttct tgctttgtag tattgtctgc aaaaattgct
    23881 gaagaactag aaaaaaacat catacctgat attaatatag ctaaattttt attattcata
    23941 tttttacctc acaatcttta aaaatttgtt atttagcata cttaataaaa tataatctta
    24001 aaatcaaatt gatactgagt ttgaagttac aaaatcaaca aataagattt ctttaataca
    24061 aaattttcct ttttatattt aacattgtat cacaaatata aaaaggaaaa aactttttaa
    24121 aaagggtata cactattcat tttttatctt ataaataagt ctttaccact gttttatata
    24181 ttttaaaata gacacaagac ctatttctaa atcttttata aatattttag gtgaaatttc
    24241 tttttaaaca tatctaaaaa gtatttgtag taatattaat ggactgaatt tcttaaagta
    24301 tatatttctt tgcatgttgg agtttgtgaa gtttgagaat tggagggtga aagaaacatg
    24361 caatggatta atattaaaga actgtatttt tataatattt gtcttaaaaa taaaaaaatc
    24421 tattgacttg aagttaactc caagtagtaa agtataatta tacaagaatt atatacttgt
    24481 aagaatagtt aaagtagaga gaaaaaacaa acaagtttta aagggggaag taaaacaatg
    24541 gaggcaaaaa aattaggatt tggattaatg cgtttaccaa taaaagatga aaatgatgtt
    24601 acaactattg atatggaaca tttaaataaa atggtagaca cttttttaga aaggggattt
    24661 acatattttg atacagccta tgtgtatcac atgggaaaaa gtgagatagc acttagagaa
    24721 tctctagtaa aaagacacaa aagagagagt tttactatag ctacaaaact tccattaatg
    24781 gctttaaaga aaaaagaaga gcaagaaacc atttttaatg agcaattaga aaaatgtggt
    24841 gtagactatt ttgactacta tcttttacac aatataggcg tgtcacatta tgaagtagct
    24901 aaaaaatttg atagctttaa gtttattgaa gaaaagaaaa aagaaggaaa aataaagaat
    24961 attggttttt cttttcatga tagtgcagaa ctattggata aagtcttgag tgagcatcca
    25021 gaagtagact tcgtgcaatt acagattaat tatttagatt gggataatga aagcatacaa
    25081 tctagaaaat gctacgaggt ggcagagaag cataataagc ctgtaattgt tatggagcca
    25141 gtaaaaggag gtacacttgc aaagatacca gaaaaagcag agaaattatt gaatgactac
    25201 aatccagata tgtcaatttc atcttgggca atacgttttg cagcaagtaa agataatgtt
    25261 atgatggtct taagtgggat gtcaaatatg gaacaattat tagacaatac aggatatatg
    25321 caaaatttca aaccattcgt acaagaggaa tataatatta tagatgaagc agtagagatt
    25381 atcaacgaat caattttagt tccttgtact gcctgtcagt attgtgtaga aggctgtcca
    25441 aaaaatatcg ctattccaaa ctattttgcg ctgtacaatg cagaaaaaca agctcttaat
    25501 actggatttt caacacagat ggtttactat aataattata ctaaaactta tggaaaggcc
    25561 tcagattgta ttgaatgtaa acaatgtgaa gaaagctgtc ctcaacacat taaaattata
    25621 gatgctttaa agaatgtagc agaaaccttt gagaaataat tcaatctata caatatcaag
    25681 aggagaagat atgtatgacg atttcagaag ttagtaaaaa atacgagctt tcagcggata
    25741 ctttacgata ttatgaacga attggtttaa taccacctgt aaatagaaat aaaagtggaa
    25801 ttaggtcttt tacagaagaa gattgtgagt gggttaattt cataaagtgt atgagaggtg
    25861 caggtctttc tattgagaca ttaattgagt atgtagctat gtttcaacaa gggagcagta
    25921 ctatcaaagc tagaaaagaa cttttaatag agcagagaaa ccaactagca aaaagaatag
    25981 aagaaatgca aaaaactttg gaacgattaa attttaagat tgatagatat gaagaaggta
    26041 ttattgagaa agaaaaagta ttaaagagca gtagaaataa gaaaatggag gcagtatctt
    26101 atgagtaaaa atgtattaat agtatcagct agtcctagaa aaggtggaaa ttctgactta
    26161 ttgtgtgacc agtttataca aggggtatta gaatctaaaa atcaagtgga gaagatattt
    26221 ttaaaagata aagatattaa ctattgtact ggatgtggtg cttgttatga aaaagaagca
    26281 agttgttcac agacagatga catgggagaa attcttgaaa aaatgattgg ggcagatgtg
    26341 attgttttag cgactcccgt ttatttttat actatgaatg cgcaaatgaa aactatgatt
    26401 gatagaactt gctctagata tactgaaatt tgtgataaag atttctattt tatcgttgca
    26461 gcagcagatg aaaatgtggc tgatatggaa agaacaatag aaggatttag aggatttaca
    26521 tcatgtctaa atggttctaa agaaaaaggt atagtatatg gtgtaggtgc ttggaatgta
    26581 ggtgatatta agggaagtaa ggcaatgaaa caggcatatg aaatgggtaa gttggcttag
    26641 tatgtttgta taaaattgta taaaatcgaa tatattctta tcatttcaac aataaaaaga
    26701 ggcttatcta tattttgaga cagcctcttt gtgctatatc atttattttt attctatatc
    26761 cttattatac cctttatttt tttatgccta tttaagtatc tgttgttact catatatttt
    26821 aataattacc cttaaaaaac tatacagatt cctgcataat attattttct ttagtaatta
    26881 atttttctag gtttttccaa tccatttctt ccatatttgc aaaataatga ccacttccta
    26941 tgcaagtcag ataagacttt ccatctaaat caaacgaaga acctccaata cactttgaat
    27001 tacatttctt acaagtacga gttttagtta tactatcttt aatagtttta gagcattcct
    27061 ccacagcatc tttatatgta tttagatgaa aaagatttgc tttaactctc catttttttt
    27121 cattacattc aatagtaaat aagctacgtt ttggtttctt agttgaatat actattttgt
    27181 aagaacgagc acctgttttt ggttcaattt tacaatctaa tgatttacca agttcatgta
    27241 aatgacatgc aatatcataa tctttaccac ttagtacttc taacatttct tctgttgttt
    27301 ctgccgttaa ttcacttttg ctcatattaa aaaaatcccc ctatataagt aataatttca
    27361 catgaaaata attaatataa gtatattaaa taaaactatt aaaatcaata aaattactac
    27421 aatcttacat tattgggcat agaatgagag ctaatgattt attagaaata agacttaata
    27481 gtatgaaaat ttacttagac gtggtacaat tctataaaag atttaaaact aggaaaggag
    27541 aaagttgttg agatacggcc aatataacaa tttatttata agttgtaaat ttattatgag
    27601 attaattaga ttgttaagta atactatcaa aaggtagttt tttggatttt cagcaacagt
    27661 ggtagatatt gataattttt tggttattat tatatttagc tgttatattt tcaataggct
    27721 ctatgataac tagggtaaat aaggtatggg taaaggtttt attatttata atagtgggat
    27781 tgtcaactat agttctttca tatgtaataa cttgataatt taagattgaa aaataacttt
    27841 aatctgtaaa ttctcaaaac aagaattctc atgttggtaa atttctagat atgagaaaaa
    27901 aattgacatc ataaaaaagt aattttaaag cattttttta cgaaaaaatt cagaagtaac
    27961 tattttgaat gacttcacaa aattttatat ataaataaag tttggcacgt taattgcttt
    28021 aataaaagtt aacaagacac aaataaacat aatcatctaa gtattgatta tttgaagttt
    28081 tagatttgaa atttaatcta aaggttcgta ttaatattgg tgtttaggaa attttatttt
    28141 cctgtaaata taacttaata ataggaggtg aagtcatgtc aaaagtagta atagctacag
    28201 agaaagctcc aggagcaatt ggaccatatt ctcaagcaat tcaagttgga aacatagttt
    28261 ctacatcagg tcaacttcca atagatatga agactggcga gttagtatct gacccaaaag
    28321 aagcaacaaa agcttcttta gataatgtaa aagcaatatt agaacaagct ggtacaagta
    28381 tggataaaat atttaagact acagtattcg taaaagattt aaaggatttt aatgatatta
    28441 atgaagtata tgctacatat tttaaagaaa atccaccagc aaggtcatgc gtacaagttg
    28501 ctcaattacc aaaagatgca gttttagaaa ttgaagcaat ggcagtatta taagtattat
    28561 aacaataaaa ccaataacaa aactaataaa aattaaaaac taataaaaaa agtctacaat
    28621 taaaaaagtt tatattaaaa a
//
LOCUS       DAELSY010000034        27796 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.034, whole genome shotgun sequence.
ACCESSION   DAELSY010000034 DAELSY010000000
VERSION     DAELSY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27796
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.034"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..498
                     /locus_tag="KPG09_001376"
     CDS             <1..498
                     /locus_tag="KPG09_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG0965997.1"
                     /translation="VSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNSFFDGGSIY
                     RVEFKNSKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVK
                     GDNLVFEYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDEIRGAIFDVSQAIDLTKLM
                     GIIIK"
     gene            639..1910
                     /gene="brnQ"
                     /locus_tag="KPG09_001377"
     CDS             639..1910
                     /gene="brnQ"
                     /locus_tag="KPG09_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG0965998.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAIFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            2112..3299
                     /locus_tag="KPG09_001378"
     CDS             2112..3299
                     /locus_tag="KPG09_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBG0965999.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMTKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(3529..5553)
                     /locus_tag="KPG09_001379"
     CDS             complement(3529..5553)
                     /locus_tag="KPG09_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0966000.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGEVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKRAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            5943..6611
                     /locus_tag="KPG09_001380"
     CDS             5943..6611
                     /locus_tag="KPG09_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966001.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLASNFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            6613..7098
                     /locus_tag="KPG09_001381"
     CDS             6613..7098
                     /locus_tag="KPG09_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG0966002.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            7159..8280
                     /locus_tag="KPG09_001382"
     CDS             7159..8280
                     /locus_tag="KPG09_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBG0966003.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMQFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            8561..9751
                     /locus_tag="KPG09_001383"
     CDS             8561..9751
                     /locus_tag="KPG09_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0966004.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDIVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLVRKGFKH
                     TPLQSLCPEYKDKIITCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    9885..10140
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10199..11200
                     /locus_tag="KPG09_001384"
     CDS             10199..11200
                     /locus_tag="KPG09_001384"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBG0966005.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            11304..11693
                     /locus_tag="KPG09_001385"
     CDS             11304..11693
                     /locus_tag="KPG09_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBG0966006.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIIKKDKHSNIIENVEFSIKDEEE"
     gene            11854..13062
                     /locus_tag="KPG09_001386"
     CDS             11854..13062
                     /locus_tag="KPG09_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBG0966007.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(13149..14225)
                     /locus_tag="KPG09_001387"
     CDS             complement(13149..14225)
                     /locus_tag="KPG09_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0966008.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            14454..14981
                     /gene="hpt"
                     /locus_tag="KPG09_001388"
     CDS             14454..14981
                     /gene="hpt"
                     /locus_tag="KPG09_001388"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG0966009.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            15070..16002
                     /locus_tag="KPG09_001389"
     CDS             15070..16002
                     /locus_tag="KPG09_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBG0966010.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIVGWMLGIFFTVIF
                     IKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            16064..17290
                     /locus_tag="KPG09_001390"
     CDS             16064..17290
                     /locus_tag="KPG09_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0966011.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTRFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINANTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            17374..17595
                     /locus_tag="KPG09_001391"
     CDS             17374..17595
                     /locus_tag="KPG09_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG0966012.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            17784..17954
                     /locus_tag="KPG09_001392"
     CDS             17784..17954
                     /locus_tag="KPG09_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966013.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(18047..18508)
                     /locus_tag="KPG09_001393"
     CDS             complement(18047..18508)
                     /locus_tag="KPG09_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966014.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQHFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            18691..19158
                     /locus_tag="KPG09_001394"
     CDS             18691..19158
                     /locus_tag="KPG09_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966015.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMIAFNLNMILGIGVFLIGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            19201..20964
                     /locus_tag="KPG09_001395"
     CDS             19201..20964
                     /locus_tag="KPG09_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBG0966016.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDTIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMKDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            20972..21580
                     /locus_tag="KPG09_001396"
     CDS             20972..21580
                     /locus_tag="KPG09_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0966017.1"
                     /translation="MDSNDKKGLKSTEIIIINGSPRINKNCSFIANKVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRNKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            21652..22221
                     /locus_tag="KPG09_001397"
     CDS             21652..22221
                     /locus_tag="KPG09_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG0966018.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            22739..26278
                     /gene="nifJ"
                     /locus_tag="KPG09_001398"
     CDS             22739..26278
                     /gene="nifJ"
                     /locus_tag="KPG09_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBG0966019.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHACIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRIQV
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKVDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVLEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
     gene            complement(26670..27596)
                     /locus_tag="KPG09_001399"
     CDS             complement(26670..27596)
                     /locus_tag="KPG09_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBG0966020.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPNNNKPESNKPEDN
                     NNSDSTNKPDDNNNSGSTSENFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKK
                     SQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGH
                     RKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
BASE COUNT        10403 a         3104 c         4819 g         9470 t
ORIGIN      
        1 gttagctttg aatcttgtat atttagaaat gtatcctttg aaaattgcaa tttaaaaaag
       61 attgatttac ttgatgtggt atttgaaaac tgcaatttat caaattcatt ttttgatgga
      121 ggaagtatat acagagttga atttaaaaat agtaaattga taggagttag atttgatgat
      181 tgtatactta aaaacgtttt gttcaaggat gttttaggta agtattctaa cttttcattc
      241 tctaaattta agatagtttc tatcttagat tcaaattttg aaaattctgt atttcaagaa
      301 gtaaaaggtg acaatctagt atttgagtat acaaacttaa aaaaagcaaa ctttaatgga
      361 actaaacttt ctggcataga ttttaccact aataatattg agggaattga gattggtata
      421 gatgagatta gaggagcaat ttttgatgtg tcacaagcaa ttgatttaac gaaattgatg
      481 ggaattatca taaaatgaaa aaaatattca taaaataaat tgggaaaacg ttaacaatgt
      541 gtgaattttt accagattga catttttctg aaaataaagt aagattatct taatattcaa
      601 aatattttca aagggggtaa tttagtgtga gtaagcagat gagagatatt tttatagttg
      661 gttttgcttt attttctatg ttctttggtg caggaaatct tatgtttcct ccatttttag
      721 ggatggaatc cggcaaagac tggttaattc ctcttattgg atttgttgtt gcagatgctg
      781 gtatagcaat gcttgttatt atagcaacag caagatgcaa gggaagcatg gatgatgtac
      841 ttaggagagc tggtaaaggg ttagcaagag taatgagtgt agctgctcta gcatgtttag
      901 cacttcttgt aataccaaga acttgtgcaa caacatatga aatgggtatt atgcctatat
      961 ttggagacca tggaccagta gctaaagcta tattttcaat aatcttcttt gggttaactt
     1021 tagcatttac tattaggtct tctaaggtta tagacataat aggtaagtat ttaactccag
     1081 cgttattatt agtattattt gttttaatag taaagggtat aatttctcca gcaggaccta
     1141 tgagtcctga gcatatgata gataaaaatc tatttggtga aggtatatca caaggttatc
     1201 aaacaatgga cgctctaggt gcagcatcta tggcaactat aatgttgatg tctataatag
     1261 ctaaaggata tacatcagat aaagaccaaa taggaatgac tattaaagct ggttttgtag
     1321 cctgtgtatt tctagcagta gtatatggtg gtcttactta tttaggtgca acagtttcaa
     1381 ctttatatga tactagtata cctcaagcat ctttattagt tgaaataact agtatattgt
     1441 tatatggtca agtaggaaag gtaattttag gtgtaattgt ggcacttgcc tgtctgacta
     1501 cttctacagg tttaacagca tcaatatcaa cttattttga aggaataagt aaggcgagtt
     1561 ataaacaact ggttataggt atatgtgtag ctagtatgct tatatcaaat ttaggtgttg
     1621 attctataat agctatatca gttccaatcc tacaaactat atatcctgtt ttattagcaa
     1681 tagttgttat ggagttggct ggtaaacata taaagaatga taatgctttt aaaggtgcag
     1741 catatgttac attagtgatt agtctattat cagctataaa tggaatgaca ggagctgttc
     1801 catttataca aagcttgcca ctcgcaggtt tgggctttaa ctgggtaata ccagctatat
     1861 tgggtggaat agttggtaac tttataaaaa gcaacaaaca agtagcataa taatttgtaa
     1921 ttaatttaat tttataatat attaattttt agtactatgg gtaggtttaa tctaaaccta
     1981 atctacccat agtacttttt agattggatt ggaatagcat gtatataatt ttttataata
     2041 taatttttct ttgaagtatg ataaaattag taatagtgat aagaaagtac aaatttagga
     2101 ggttttaatt aatgcttaga aaaataacag catttatgct atccatatgt ttaattattg
     2161 gacttacagg ttgtagttta cttgtaggca agaaagataa agagaaaatt atagatgatt
     2221 ttaaagttgc agtggtaact caaccattat ctgaaaataa agtacaatac aatatggtgg
     2281 aagagatgac aaaagaatat gaggaagaga ataaaattga taaagacaaa gatggtcaaa
     2341 ctaaagttaa acaaactata aaacatgttg tattgccaga gaattttact tctaatatag
     2401 atagtgctat taataagata gtaaagttgg ctgacgataa agaagttcag gctattgtag
     2461 tttcaactga ccaagcaggg cttttacctg ctttacaaaa ggtaaaggaa aagcgtccag
     2521 aaataataac tatttctgcc cctatgggag atgataaaaa ccaattaagc caatttgttg
     2581 atgtgaatct aggggttagt gcagaggaaa gaggaaaagt attagctgaa cgttcaaaag
     2641 aaatgggagc aaaagctttt attcattatg cctctacaga tgatttgaaa gatgttaata
     2701 tagccaaaag attagaaatg attaaagaga cttgtaaaaa tataggattg ccatttgtac
     2761 aagttaatac tccaaatata aatactgaag aagacaaaaa caaagttaag cagtttttaa
     2821 atgaagatat tgaaaaacaa gttaaaaaat atggaaaaga tataaatgta tttggtgtaa
     2881 atgagtatat ggatgaagtt atattgacta aggctttaga attaaagtat atagttgcag
     2941 agcaaagcaa tccatcacca atacaaacgt atcctagtgt tatgggtcta aaaatatctg
     3001 aaaaagatgc tcaaaattat gataaaataa acgacatgat aagtgaaaag gctaaggctt
     3061 ttggtatgtc aaatagactt ggtggatatc ctatgccaat ggatgctttt ctaccatcag
     3121 tagcaattta tcttgctact gaaatggtga aacaagattt aacacaagaa gatgtatgtg
     3181 acccagatta tttagaggcg tttacagaat taagatttgg aatcggttca gaatttactc
     3241 ctttaacaga ggtactttat aattatcaat cagtaatttt aagccaatta atttattaat
     3301 attaaggaag tgtctcaaaa tgaacttttt agtttatgag gcactatttt tctgttaaat
     3361 caaatatatt tttatctttt taacaattaa aaagaagctt atccctatgt tgagatagct
     3421 tcttttttaa tataaataag ttttattatt ttataaaaaa tttatagaaa aataaaaaat
     3481 tataattata tataaatttt gataaagtaa agatgaatta ctataggttt aaattatatt
     3541 attttctatt atttttatgc ctttctcagt tattgatgag ccttttcttc ctttactaga
     3601 aattaagtat ccattgcgtg tcaaatattc tagcatattt cttacttcgt attctgtgac
     3661 attatattcc ataaatttca ttaactttgt cctccccatt ggtttcatgt gcaatgattt
     3721 taggatagaa atgcatatgt ttttgtcata ttggtttaaa tcattgaata tagacatatt
     3781 acttgacata tgattattat ttaattttga agatatgttt tgaggtaaat catttattgt
     3841 aagataattg gaacccatgt aagccatata ttcaacacaa tttctcagtt ctctaatatt
     3901 ccccatccaa gtgtatgaat ttaaagcttc taaaagactc ttatcgagtt ttatgttttt
     3961 atagggaagt tcttttaaaa atgattctaa aagaagaggt atatcttcta atctctttct
     4021 aagaggaggt aaatctaaag tgaacatact tatcctataa aataaatcca tcctaaattt
     4081 attttctata attaattttt ctaaatcttt atttgtggca gctaatattc tcacatcaat
     4141 tggaataata cttgtaccac caatttttct aatttctttt tcttgtaaga ctcttaaaag
     4201 tttaacctgt aaatcatgtg gcatatctcc tatctcatct aaaaatatag tgccagtatg
     4261 agctaattcg aataatcctt tttttccacc tttttttgct ccagtaaaag aaccatcttc
     4321 atatccaaat agttcacttt caagtaattc acttggtaat gaagcacaat ttattgctac
     4381 aaaaggttta ttttttctat ttgaatgatt gtgtattgac tgagtaaagg cttctttacc
     4441 tgttcctgtt tcacctgtta ttaatatagg attatctgta agagccattt tttttgccct
     4501 tttaatacaa tcttttataa tagaactatt tccaataata ttattaaatt tatatttagc
     4561 tacataacct cttttgacag aatcacttct tattttttgt tctaatattt ctatattgtt
     4621 tacatctttt aatgtaatta agcaaccctc tatattttta taaagataaa atggtttttt
     4681 agaaagtatg aattttttat ctatttcgtc tatgtatata atcatattat cgatatttaa
     4741 gttctttgta attttatcta agaaactctt tggcatatat tccattaagg aaggtgattt
     4801 tataagtttt tcatctagtt ccaacatatt aagtaaagac ttatttacaa aagtaacctt
     4861 gttaaaggtg ttgaaaaata ttagaccatc acctatttca tcaatggtca tgtaaagtat
     4921 agtttttaat ttagatatat tatctaatga cgtatttaag aaatcatgac ttagtgtctc
     4981 cttggacaat ttgtatagtt tttctataaa tttctcattt tttagtttta atactactaa
     5041 taatgacata tatgtatcta atgagatttt tctccaacct aaatcaacaa tattttttat
     5101 gtattttggt ataaatctaa tctgacctgg tgttatagct gtatctaaat catatatttc
     5161 atcacaatct ggatatgtcg gtacaaaatc tatatgattt ataccaattt tatatagata
     5221 agtagccatc tcaaatgctg aagttttatt ggttgtcata agtaacgatt ttgtacctgg
     5281 aggtagtttc atcatttttt catatccaag ttttgaaagt gctttcgttc catgaataat
     5341 ttcaatatct tccttaaaat gttttattaa aaaattacta gttaaaggtg atagagaaag
     5401 cactacctct ccctctaaat tttcttttat tccatcaagt atattttgtg tttctatgat
     5461 tatttcgtct ccaaaaaagt ttgttaattc ttctttgtag tggtcactat ttaatttatc
     5521 atatgttata aggattaact tctttttatc cattttattt ctcctaacaa aatagtattt
     5581 atttttaaag tatttaaagt ataaggtaag ttatattata caacaaatag tagtataaat
     5641 ttatatattt gattaaataa taggtttttt taggatttta tcctataaaa acctatttta
     5701 aaattatttt tgcaagataa ggaaaatagc tttttatatt gtaatgtata ttttaaatag
     5761 ttaataaaaa aatattatat ttttaaactt gaaaagtaag taattataag ctatttaatc
     5821 gctggaataa atttacaata aaaaaataaa tacaaaggct ttatcattaa ggtattggca
     5881 taagttttgc ttattaaaca agtatactaa taaaattcat ttaaacagga gggagaattt
     5941 atatgagtaa attagaatca acaacagaaa aagttgaggt aaaatggtat gggtatgtag
     6001 cattaatatt gggagcatta tttttttcag gaatatttaa agatgcacca ggaccattaa
     6061 aagtattgga ttttaacaat gtattaggtt cttttggaac attgggaaca attaatgatg
     6121 gtgtaggtac attagcttca aactttagag gtgatggagg tactggccct cgagatggat
     6181 ggctatatgc attgacatta ataccatcag ttatgtttgc acttggagta gttagagtta
     6241 tagaccactt agatggaatg aaggcagctc aaaagcttct tagtccacta ttaaagccat
     6301 tattagggtt acctggattt gcaggactaa ctttgatagc tagtttacag tctacagatg
     6361 cagctgcatc tatgactaaa gaacttaaag atgatgggta tatagatgaa aaacaaaaag
     6421 ctgtattttg tgcatttcaa ttttcaggtg cgtctgcaat tacgaatttc tttgcttctg
     6481 gagcagcact atttccattt ataggagatg taccaatatt tataccactt gctttaatat
     6541 taattatgaa gtttgttggg gcgaacttac ttagattata tttaaataaa tttgacaagg
     6601 gggaagctta acatgagtga ggtagcaaaa aaagaagtaa aagttaaaaa aggaaatgtg
     6661 atagatgcgt ttattggagg agctagaaat ggattccaaa taagtacaaa ttcaatggct
     6721 ccaaatgtca tcttcggttt tgctataatt tctgtgttca atttaacagg tcttttggat
     6781 gtaataggga caatatttac acctgtaatg agtatctttg gacttccagg tgttgcagca
     6841 acagctataa tgacaatatt tatatcaatg ggaggagcat ctggagtaat cgcaggttta
     6901 tttacagcag gtcaaataag tcaagcacat gttgcaattc tattgccaat gttattgctt
     6961 tcaggagcgc agatacaatt tatggggaga cttttgggaa cagctgagtt aaaaactaaa
     7021 tattatccac atatgtttgt tatagcaact ttaaatggtt gtattgcaat gcttataatg
     7081 aattatttcg tagtataggt aattataata aaaatttaat ataaatggaa agtttaaatt
     7141 ttaaggagga gctagattat gggaactaac aaagattata ttgagtatgt acataaagtg
     7201 tatgattatc tacacactat accagaaaaa ggatttgaag aatataagac atcagagttt
     7261 gttttaaaag agcttgaaaa aatggggttt gaaaatatta ataaaggagt tgcaggaact
     7321 ggtattatag taactataga ttctaaaaaa gaaggaccag tatttgctat aagagcagat
     7381 atggatgcat tggaatttat catagatgga gaaaagaaaa tgatacatgc ttgtggtcat
     7441 gatggtcata cttctatgct tttaacagct gcaaagttaa taaaagagag aaatttagta
     7501 aaaagaggta agttaaaaat actttttcaa ccaggtgaag aaaaattata tggagcatta
     7561 agagtaattg attctggttt gttggatgat gtagaagaaa tggtagggat gcatttaaga
     7621 cctggacaag attgtaggtt gggaaaggcg atacctgcaa tgtaccattg tgctagctgt
     7681 ataatgcaat ttacgattaa aggtatcact tgtcatggtt ctaaaccaca tttaggagta
     7741 aatgcagtag atgctgtctg tttagcagtg aatgcaataa atgcaataag agtaaatcca
     7801 gctataccaa attctataaa agtaacaaat atatcagtta agggagaaac ttataataat
     7861 ataccagatg aagcatttat ggctttagat ataagggcag aaagtaatga tataatagaa
     7921 gaattaaaag aaaaagcaat acatgctgta aaaactgctg taagttctat aaatgcagaa
     7981 gctgtactaa tttccaaaga tggagtacca gcagcagaat ttgatgaaga acttgtaaac
     8041 ttggctgaag aaactataaa agatgtattg ggagctgatg catctatagg tatatttaaa
     8101 aattctggtg gagaagattt ccattattat acacaaaaac taaaatgtaa aactacatat
     8161 attggattag gagctgatgc tacaccagga tttcataatc caaatgtaac atttgatacg
     8221 aaagctttag aatatggtgt agatatatgg tgtagattgg ttgaaaagag attgggataa
     8281 tatccaaact aataattcta catttaaaat tataattata gaagttatag aaaaagattg
     8341 cttatgttaa ttagaaaata attaatatga gcaatcttat tttgatataa tgaaactgat
     8401 tttggaatat tgaaaaaata tgatggaaaa gtaattatat tttaaatcta ttggacaaaa
     8461 aagtgatatt attataatat taagaattgt aaaataatac caaagtgcat ctagtaatgg
     8521 acttaacaat tataaaaatt ttattcttag gagggaaaaa atgagtaagt ataattttga
     8581 cagagtaata gatagagtag gaacagattg tgtaaaatgg gattttagaa ctaattgttc
     8641 aacaaaagca caaaaagatg gtttgccttt ttggattgct gatatggatt ttgaatgtgc
     8701 agaaccaata atagaagcat tacgtaaaaa agttgaacat aaaatatttg gatatagttc
     8761 aaatgatagt gataaatact ttaatgcagt ttgtgattgg tataaaagga gatttaactg
     8821 ggaaataaat agaaaagata ttatttttag tcctggtgtt gtacctgctg ttgctatttt
     8881 agtaagaata ttaactaatt ctaatgaagg tgtaatagtc caaaaaccag tgtattaccc
     8941 atttgaagct aagataaaga gcaataatag ggacattgta aacaatcctc taatatatga
     9001 gaatggtact tataggatgg attatgatga tttagaagaa aaagctaagt gtagcaacaa
     9061 taaagtactg atactttgta gtcctcacaa ccctgttgga agagtttgga gagaagatga
     9121 attaaagaag gttgtagaaa tatgtaaaaa atatgaccta tggataatag ctgatgagat
     9181 acattctgat ttagttagaa aaggatttaa acatactcct ttacagtcat tatgcccaga
     9241 gtacaaggat aaaataatta catgtatagc tccaagtaaa acatttaatc tggcaggaat
     9301 gcagttgtct aatataatag tgaataatga tgaactaaaa aagaaatatc aagaagaggt
     9361 tacagctgtt ggagttgcaa cttcaccaaa tccctttgct attgtagcta caatagcagc
     9421 atataatgaa agtgaagact ggcttaatga attaaatgat tacttagata ataatataca
     9481 atttattgat gaatacttaa aagaaaacct accaaaagta aaacttgttt atccagaagg
     9541 aacatatcta gcatggttag attttactgc ttatggatta aatgaagtag aacttgagga
     9601 tttaatgttt aaaaaagcta atgtattatt tgatgaaggt tatatatttg gaaaagaagg
     9661 aataggattt gaaagaataa atgtagcttg tccacagtct ttattaaaag aatgtatgga
     9721 cagattaaag actatatttg aaaatttata aagtataggt aaaaatttat aaagtgttta
     9781 aaaataaaaa aataataaac ttgacaaatt ttaactaatc acttatcata ctattatgaa
     9841 taaatagaaa acataagaga gctaaatttt attgaattaa actcttaaag actttgaaag
     9901 gaagagtagt tatagaatgt aattaaagcg agttggtgat ggtgtgagac caatatgaag
     9961 tctataatga agaacatcct ggagttgcta accgaaattt taaaagagta ggcttagtcg
    10021 gaaccaaacc gttatcatat tggggtgtat cgagtaatat ctgtacattg tgagctgggt
    10081 ttgaatttat ttcaaactaa tcagggtggt accgcggaaa agcaaccttt cgtccctttt
    10141 atatagggat gaagggtttt tttaatggaa aaatatataa atatagggag tgaatgatat
    10201 gagcacaata ataccaaagg attataaaag tagtttgaac gtaattgata cacaaatagc
    10261 tataaaaaaa ttaaaagatt tttttgaaat gagactaagt aatgaattaa atcttataag
    10321 agtttcttct ccgttatttg tattgccaga aacaggtgca aatgataatt taaatggaga
    10381 aaaggctgta agctttgata taccatttat gaataaaaat gcagaaattg ttcaatcact
    10441 tgcaaaatgg aaaagattag cccttaaaaa atatgggttt gaagttggta gtggtttata
    10501 tacagatatg aatgcaataa gaaaaaatga agaattagac aatatacatt ctctttatgt
    10561 agaccaatgg gattgggaat tggttataga taaaagtagt agaaatgaaa agactctaaa
    10621 agatgttgta aaaagattgt atggagtatt taaagatact gaaatttttg tatgtagtat
    10681 gtatgaaggt ataaaagaaa tattaccaga agaaataact tttataacat cacaagagtt
    10741 agaagatatg tatccagaat tatcaccgaa ggaaagagaa gataaaattg taaaagagaa
    10801 gaaagcagta tttataactc aaataggaaa aacattaata tcaggagaaa aacatgatgg
    10861 aagaagccct gattatgatg attgggaatt gaatggggat atattatttt acaatcctgt
    10921 tttagattca gcattagaat tatcttcaat gggaattaga gttgatgaag aatctcttga
    10981 tagacaattg aaacttgctg gatgtgaaga gagaaaagag tttgactatc ataagatgct
    11041 tttaaatggc gaacttccgt atacaattgg tggaggaatt ggtcagtcaa gaatatgtat
    11101 gtatttctta caaaaagctc atataggaga agtacaagtt ggagtatggc cacaggatat
    11161 gattcaaaat tgtagtagtg caggaattga attattataa tttaaatata aactaattga
    11221 taaaatttat atctaatatg ttcatcaaat taattatatc ggttataatg tagaataacc
    11281 tataattata agaggtgata aatatggagt ttttaaaaat aatcattaat atagttttag
    11341 atattttaaa aaaaattttg gtcagattta aaaatgctaa atttggtttg gtttttgtat
    11401 ttgatttact taaactacct gattttatga cagataaaag aatcaatata gttgataaaa
    11461 taaaggtaat ttctgtatta atttttacta tatcctactt tgtatctggg gttgatataa
    11521 ttccagagat gattgcaggg gcatttggtt ttatagatga tgctatagtt ttaatttgga
    11581 gtataggtat agtgaatgaa gaaattaata agtatagagt tattataaaa aaagataaac
    11641 attcaaatat aattgaaaat gtagaatttt ctataaaaga tgaagaagaa taacaattta
    11701 tatatgttat tctttttttt attaacaaaa aatggttaaa ttgttgacgg agaggtaaaa
    11761 ctctattatc ataataagtg gatatccaat atttaactaa gtattcaatg tgcaagaatg
    11821 cttaatttaa aaaagttatg gagggtaaat attatgaaga aaaaaatagg tttagtgccc
    11881 aaactaatca tcggtatcat agtcggtatt ttaatcggaa gttatgcacc agaaataata
    11941 gtacaaatat tagtaacagt tagtacctta ttctcagcat tcctaaaatt tgttatacca
    12001 tttataatta taggttttgt aacagcaggt atagctgatt tagcaacagg tgcaggaaag
    12061 ttattgggga taactacggg aatagcatat ggttctacct taatagcagg attattatca
    12121 tttgcagtat ctacattaat cttcccaaat tttattgatg caagtgttgc aagtcaaatt
    12181 ggagacccag aggcaggaat gttagctcca atattcacaa ttccattaga gcctatggta
    12241 gatgtaacag ctgcaatcgt atttgcattt gtaatgggac ttggaatatc agctttaaga
    12301 aatcatggaa aaggagagac tttatttaat ttcttccaag aatttcaagg aatcgtaacg
    12361 aaaacgttgt caacagtgat tataccttta ttacctttat acatagcagg aacttttgca
    12421 aatataacta tagcagggga agtatggaca atattagggg tattctggaa agtatactta
    12481 gtagtaatac ctttacattt tgtatatatg gcatgtcaat tcacagcagc aggtgtattt
    12541 tcaggaaaaa atccagtaag aatgttaaaa aatcaagtac ctggatattt aacagctata
    12601 ggaactcaat cttcagcagc aactatacca gttaatgttg gttgtgcaga aaaaaatgga
    12661 gtaagtaagc aaataagaga atttgttgta cctctttgtg caacaataca tttatcaggt
    12721 agtatgatat caataacagg atttacaact gcagttttaa tgatgaatgg tatgccacat
    12781 ggaataggtg tattattccc ttatattgca atgttaggta tagcaatggt tgcagcacca
    12841 ggagcaccag gtggggctat aatgagtgca cttcctttcc taccaatggt tggaatacca
    12901 tcagatggtg ggcttgcaag tttaatgata gctttatact taactcaaga cagttttggt
    12961 acagcatgta atgtatcagg agataatgca atagcagtag tagtagataa aataaatgat
    13021 ggaatgagta aaaaaggaaa caaagaagag caaagtgctt agttttttaa taatcaaaaa
    13081 tgagttatct caaatagaga tagctcattt tttatggtat aaaaaaatta taaaataaag
    13141 ggattttttt atttaaaaat tgctttaaga gatgatatta ataatttatt ttcattaggt
    13201 tttaatatac atactctaaa gaaatactca tctagacctt caaatgaaca acaatctctt
    13261 attattattt tttgtggaag cagttgttct cgaagcgatt tagcagttaa ttcctttgtt
    13321 ttgattttac acaatatgaa attaccttta gtattgtata tatctaagct ttttatattc
    13381 ttaagctctt taagcaggta atctctttca gtgttcatta aggaaatagt atttgatata
    13441 aaatctaaat cactaaacat gatttccccc attttagaag ctattatgtt tatattccat
    13501 aaatctagat ttttatttat ttcatttttg acattagtgt tagatataag accatatcca
    13561 agccttattc caggcgttga aaaaaatttt gatgtacctc ttataacgaa taaattagaa
    13621 taatcatcaa ctaattgagt acaagagtat gtatctgtat cagtaaactc tacataagtc
    13681 tcatcaatca ttaaaaatga gtctgtattt tttaaaattt ctctcacttc tactttagta
    13741 aatgcaaatc cagttggatt actaggatta cagattacta ccaaatcata atctttagca
    13801 ttgatagttt ttataagatt ttctaaattt atatgaaaat tatcttcttc ttttgcaaag
    13861 tatttttcta tagaacaatt aattttagat aactcttttt catactctga ataggctgga
    13921 gagagtaaaa gagcctgttt aggattaatt gtatgtataa atgatgaaat tagttctgta
    13981 gctccactgc caagtactat attatctata gaacatttgc agtaattgga tatagaagtt
    14041 tttaagtcta tgtaatcagg gtcaggatac atagatacca tatctatatt gtttacaatg
    14101 tattgtttgg ctaaacttga tgtaccaaaa ggatttatat ttgagctaaa atccataaat
    14161 tcttcctttg aaaagccata cttactagat aaactatata aattagctcc atgatttgtg
    14221 ctcataaaag tacctccaaa aataaaatta tataatcgat aaaaataact gattaatata
    14281 tgtacatata gattataata caaaggaatc gttattccaa atttttaatg gaaaaagcaa
    14341 aaaagtatgg ataagctgtt aatttaggtc taaaaatgat gaaaaagatt ttaattgata
    14401 ttttggtatg ctataatata taagttaata gaaggaaagc gggaggatac aaaatgtaca
    14461 aagttacagg aaaaatgttg acagaagaac aaataaaaga aaaagtttat gaattaggaa
    14521 aaaagataga agaagacttt aaaggcgaag atttactagt ggtaggtata cttaaaggag
    14581 ctagtgtatt tgtttcagat ttaataagat gtatagattt ggatgtaaat atagatttta
    14641 tgagtgttac aagttatgga aactcaacag agtcttctgg aacagttaaa atattaaaag
    14701 atttagatgt agatattgaa ggaaagaatg tgttgattgt agaggatata atagattctg
    14761 gtttaacatt aagtaatcta gttgcagctt taaaaactag aaatccaaaa tctttaaaac
    14821 tatgtactct acttgacaaa cctcagagaa gaaaagctaa tatacctgta gactatgttg
    14881 gatttgttat agaagacaaa tttattgttg gttatggaat agactatgct gaaaagtata
    14941 gaaatttacc ttatataggt atagttgagg atgtagaata gatttaaatg tgaatcaaaa
    15001 gcactaattt ctaagggagt tagtgctttt tagttaatta tataaaattt tagtaaagga
    15061 gacatctata tggatattca gttaaatata ttattatttt tccaaaattt aagaaatcca
    15121 attttgaact ttatatttct tgtctttaca ataagcacag aagcaccgtt attaatatta
    15181 attacaacaa ttatatactg gtgtataaat aaaaaatgtg gacagaaaat actgtttgct
    15241 ataattggaa attttgttgt aaatttaggc atcaaagagt ttgtaaaagc tcccagacca
    15301 ataggaataa aaggtcttga atcattgaga gtttctactg ctggtggtta ttcttttcca
    15361 agtgctcata ctcaaactgc aacaacattt tgggttagta ttatgactat atttaaaaag
    15421 agatatcttc atttaatagg tgcagttatg gttttaggag taggattgtc taggctgtat
    15481 ttagctgttc attggccaat agatgtgata gtgggttgga tgttaggaat attttttaca
    15541 gttatattta taaaaatatt tgactatatt gatgataata aaaattatat tttacttgta
    15601 gttttactta taccatttat aatagtagct atatttctaa atagccctga atattttgaa
    15661 aaatttggta taatagtagg atttgtattt ggatatatgg ttgaggatag atttgttaaa
    15721 tttaatacag ataataataa caaaaaaata aattttagca ataaaaataa ggggagtaaa
    15781 aatataattt tttcaagaat atgtaggttt ttggttggga tagtaacaat aggaatgtta
    15841 tatattggca ttaagttgtt tatttcaatg ttaatagtca ctttaaatat aagtgactct
    15901 actatgagta taatttttat gaattttatt aagaatacag tagttgtttt ttatggaata
    15961 gcaggagctc cagcattatt taaactttta aaattaaatt gattttaagg tatagagtca
    16021 atagtttaaa ttaaaaaaat taagggtaga aaagagtgat tatttgaaga aggtagtaat
    16081 aataggtggt ggaccagcag ggatgatagc cgcatcaact gcatgtgaga aaggctatga
    16141 tgtaacatta attgagaaaa atcataaatt aggaaaaaaa cttgcaataa caggaaaagg
    16201 tagatgtaat attactaacg cttgtgaaat agaggaatta atagaaaatg tacctacaaa
    16261 tggaaaattt ctttatagtg ctttttatac ttttacaaat gatgatgtaa taagtatgtt
    16321 taataatctt ggagtaaaga ctaaaacaga aagaggaaaa agggtttttc cagagagtga
    16381 taaagccttt gatattgtaa atgctcttga aagacaactt aaaagtaaga aagtaaatat
    16441 attattaaat tcaaaagtag aaaaaataat atctaaaaat aataaaattg agaaagtaat
    16501 tttaaacgat aaaaaagaaa ttaagtgtga ttcagttgta gtagcaactg gtggattaag
    16561 ttatccactt actggttcta ctggtgatgg atataaattt gcaatttcac aggggcacac
    16621 aattatagat acaaaaccat ctttaattgg tatagaagta caagaaagtt ttacaaaaga
    16681 tttagaaaaa ttatcattga gaaatgttga aataagggtg tttaatagca aacaaaagaa
    16741 agtttatagt gattttggag aacttgaatt tactagattt ggtttagatg gacctataat
    16801 caagtctgca agttgtagaa tgaaagatac aaggaaagaa aattacacta tattattaga
    16861 tttaaaacct gcattagatg aggaaaaact ggataaaaga gttcaaaaag attttcagaa
    16921 gtatacaaat aaaaaatttg aaaaagcatt agatgattta cttccaaaaa aactaatacc
    16981 tataataata aacttaagtg aaattaatgc aaatacggtt gttcaccaaa tttctagaga
    17041 gcagagaaaa aacttagtcc atttattaaa aaatttaaag tttacagtaa agagatacag
    17101 acctattgaa gaagctataa taacttctgg tggtgtaaaa gtaaacgaaa taaattcaag
    17161 cacaatggaa tcaaaattag tagaaggttt attttttgct ggagaagtaa tagatataga
    17221 tgcatataca ggtggattca atttgcaaat agctttttct actggatatt tggcaggttt
    17281 taattgctaa aaattacctg aattaaaaat gtttaaaatc acataatatt atcagcaaca
    17341 caaaataaat taaacagaag gaaggtaatc aacatggcaa gtaacaataa caacaacaga
    17401 acagtagtac caggagcaaa agaggcttta aaccaaatga aattagaaat agctaatgaa
    17461 cttggtatga gtaattatca acaagttgat aaaggaaacc ttactgcaag agaaaatggt
    17521 tatgtaggtg gatacatgac taaaaaatta gttgaaatgg ctgaacaaca aatggctgga
    17581 aaaagcaaca gatagttata aataattatt tataactatt ggctattagt ttagctagag
    17641 ctatctcaaa tatagaaagt aatttctaaa ttgagatagc ttttttttat taaattatta
    17701 aatagatata tttttatgta ataaatttta tgtatttgga taaaataata caaatgaata
    17761 tgttacataa ggagtgatgt aaaatgagta gtgtaaattt tgaggattta aaaaacaaat
    17821 ttattaatag tgacttagat gaaaaaataa gaatatatac aacaacagaa ggtttatcag
    17881 tagaacaatt taaagagcta ctaaaatatt atccaataca acatttatct aaactagaaa
    17941 aagcacttgg atagcagtat ttagtccaaa attaaaaagg caatttattt aatattaaaa
    18001 atatttttta ttaaataaat tgccttttta taataggttt aaattattat gcttttgata
    18061 tataattata gattctctca tttccaagtt caatagaccc agtattaata agagtaacga
    18121 ttccttctat atctctagca gttaagtatt tatatggaat taataaatta taaccagtat
    18181 tacttagtaa gttttttaat tgggttattt gagagatatt gaaacatttt gcagttttag
    18241 tagatttttt tcttaagtta gataaatttt cattttgaat atttattaaa gtttctatat
    18301 agtttctata agatatctta aaatttgtct cattgactat tttttcatta agtatactac
    18361 tgataaaatg ttgagacaat atattatctg caaatttatc tgctgttaat tttccagatg
    18421 ttatgccttt ttgatatttt ctaagaattt ttttagtata gcagtatatt ttatttctcg
    18481 tttctaaatc gagttgttct atttgcacaa aaccacttcc ttctaattat aaaaatattt
    18541 attaaaaatc tatacttaac taataaagct tcaataattt atttacagta aatactaaat
    18601 cataaaatat ttgttatttt ttattattac caagtataaa aaatattata attgtaaaac
    18661 ttacaaatat atgtaaaata tttaggagtg atgaaaatgt taaatgggat aataaagctt
    18721 ttttattctg aaattgtaaa aacaaagatt aataacctag actcaagtgg atattcttta
    18781 agagatgaat gtaaagaaat cagaaatata actctaaaga tagatagcgt attttttaag
    18841 cttttaaagt tggcttttgg ttcaatactg cttatgatag cttttaattt aaacatgatt
    18901 ctaggtatag gagtgttttt aattggtgta gtttatatac tatataaaag aaatatggaa
    18961 gaacaagtaa aagagcatat aaataatgtg aaaaataata tcgacatctc taaaataact
    19021 tttatggatg aaaaagggag aacaggtcta aatgcattga ttacattact tcttatagga
    19081 ttattgacag gatttaactg gatattagtt gctagttttg tcgttgtttt tgtttttact
    19141 ataaagaatg tatgttaaaa tactaataaa tagtttaata tataggggga gaaattgagt
    19201 atgactaatg gcagttataa aatgaagaat ttagtggaga tatccgctat ggtgacggaa
    19261 tctacagatt tttttaatat aaaagatgac ataatagaaa aaatgttaga agttgttcac
    19321 cctacaaagg catgtgtaaa tctattttat aaaaatgatt ctaaatatgc ctacttagta
    19381 tgttcgcaaa ccttagaata tgtacctcaa atttttccaa taaatagtct taaaggtgca
    19441 aaaattgatt ttgacacata tccagagtat atacatgagg ctgtaaatga aaaaaagata
    19501 atatatgtgg aaaatgtatt tgaagattct agagcagaag gtgaacgaga tttagcaaaa
    19561 gctgaaggtt atattgggag aatagttttt ccttttataa taaataatgt agttgttgga
    19621 tttatgactt gttttttaaa agaggatgat tatataacag aacaagatat agattttata
    19681 tcatctgttg catcactctt aagtttatca atagaaataa ctaataaaaa taataatgta
    19741 caaattttaa ttgataaact aagaggctca ataagtgcca taaatgaagc tactaaaaaa
    19801 ctatacctta ataagagtat tgatggcttt ttggaaaatt taagtaaaca agcgtgtaat
    19861 attactaata gtaaagaagc tttaataatt ataaatagtc atcataataa ggatactata
    19921 gttagttgtt ataatgttga gcaaaaaaag aaaacaaatt tatatcctat gattgatatg
    19981 tttttaaaga aagagtcatt agggggatac ctaaataatt taaaaccatg ctcaaaaaag
    20041 ggcataaatt tggaaagtta tatatattac aaacttcagg ataaaaatga gactatagga
    20101 tgtatagttt gtgcaaatag taaaaactat actagtgatg atttaaatat tcttagtata
    20161 ttgtccaaac aagtttctgt tgggatgcaa ttatatgaac ataatgaaaa ggaagtaaaa
    20221 cacaaagttc ttgaaaatga gttgaacata ttaaataaac aacaacagct tattatggat
    20281 aaaggtaaaa tggattgtaa tgatgagaaa gaactttatt tttaccataa accagcaagg
    20341 gtagtaggtg gagattttta ttatgctaca aaagtggatg acgaacatat agtttatatt
    20401 gttgctgatg taatgggaca tggaatggta tcaaattatg ttgttgcaat gataaaaggt
    20461 gcttttaaag tattatgcaa tcaatacaat acaccaagag aaataatgac aaatttaaat
    20521 aggatgttat atgatgaatt tgacaagatg ggagtattta caacttgttt gataggtatt
    20581 atagatacta aagaaaatat gattactgtg tctaatgcag gtcactatag tcctgtagtt
    20641 gtaaagaaag atggcacaat agaaaataat ttaaattgta aaaagggcat acctattgga
    20701 ataatgaaag atgctactta tgaaaataat actctttcaa tggaagatta tgcaatggta
    20761 tgtatgtaca ctgatggaat tttagagata aaaaattcat caaaagaaga gtatggtata
    20821 aacagattag aaaactttct aaaagaaaat tttagattaa atcaacagtt aatagtagaa
    20881 aatctaaaat tggatttaaa gaatttctca tcaaaagata attatgatga tgatatttta
    20941 atagttatgt taaaggatag gtagattgat tatggattca aacgataaaa aaggacttaa
    21001 aagtacagag attataataa taaatggaag tccaagaatt aataaaaatt gcagttttat
    21061 agcaaataaa gtaaaagcta tgctagaaga gtgtgggtta agttgtaaaa tatttaatat
    21121 ctacaatatg gatattgaat attgcacagc atgtggattt tgtgaaaaaa cagggtattg
    21181 caagtttaat gatgatatga agccattata taaaatgttt gatgattcaa aaggaactat
    21241 agtaataagt cctgtacatt ttgattcaat atcagctaaa ttaaagactg ttgttgatag
    21301 aactcaagct atatatgcaa gtaaatatgt attaaataaa ccttcaattg atagaaataa
    21361 gaaacggata ggaatgtata ttgcagtggg ggggagtaaa tcatatgatt cacagtttaa
    21421 aggtggacag attgttatgg attttttctt taagagtata aatactaaac ttttacacaa
    21481 tatatattta aacaatacag atgatgtatc ttatatagat aatcaagaat ttaaagatat
    21541 aatatataaa aatttaaaga tatatattga ttcaatataa tttgattttg taggtaatat
    21601 tatgtataat tgtattgcaa taatttaaat aattaaaagg agggaatttc aatgaaatta
    21661 ccaatagcat tatctaataa acatgttcat ttgagtcaag ctgatataga cgtattattt
    21721 ggggcgggtc atgagttaac taaatttaaa ccattatctc aaccagggca atatgcctgt
    21781 gaagaaaaag tggatttagt aggaccaaaa ggaactttaa agggagttag agtactagga
    21841 ccagctagag ctcatactca agttgaaata tctttagcag atggtttccc attaggagta
    21901 aaagcaccaa taaaagaatc tggtgatata gctggaactc caggtgttaa attagtagga
    21961 cctgctggag aagttgaaat gaaagaagga gttatagttg cttctagaca tatacatatg
    22021 agcaatgaag atgctgctaa atttggtgta actgataagc aaatagtaaa agttaaaaca
    22081 tctggaccaa gagcattagt atttgaaaat gtattagtaa gagctagtga tagctttaat
    22141 ctagaaatgc atgttgatat ggaagaggga aatgcagcag gcgttaaaaa tggagattta
    22201 gtagaattaa tagttgatta atattataaa tagactttat gctagtagat attttatttg
    22261 ctagcatatt tttaaatgta ataaatttct ataggataaa tttaatcgat tagattaatt
    22321 tataaaataa aggggtatta taaaaaatgt aataaaaact tacaaaaata tgatgtaata
    22381 tggtaaaatt atatatataa aaatttacat ataataaaga taattatttt actttttata
    22441 ctaatgtaaa caaatagata aaaaagtttg aaaagtctaa cttaatttat tatatattgt
    22501 atacaaaatt atgaaaaaaa cttaacatat ttaattttaa aaaaaatcta cttaaatatg
    22561 taattaattt acaattttca aaggaatttt atctaatctg tagtattata ttagaagata
    22621 gagtatttgc gcgactatat gtgtgtttat atttttgttg tgtaaattat atgtaaatat
    22681 aaaaaaatag acttgttata aacaagtaca attgtagcta tcaaggagga tacatattat
    22741 ggctaagttt atgaaaacac ttgatggaaa tacagctgca gctcatgttg cctatgcgtt
    22801 tacagatgta gcagctatat atccaatcac accatcttca actatggctg aagtagttga
    22861 tgagtgggca tcacaaggaa gaaaaaatat atttggacaa acagttaatg ttgttgaaat
    22921 gcagtcagaa gcaggtgcat caggaacatt ccacggttct ttacaaggag gagctttaac
    22981 ttctactttt acagcatctc aaggtttatt attaatgata cctaacatgt ataaagttgc
    23041 aggagagtta ttaccaggag tattccatgt aagtgctcgt gctttggcat ctcaagcgtt
    23101 atcaatattt ggggatcacc aagacgttat ggctgctaga caaactggat gtgtattatt
    23161 agcttctggt tcagttcaag aagtagctga tatagcacct gttgcacatt tagctgcaat
    23221 agaaggtaga ttaccattta tacatttctt tgatggattc agaacttcac atgaaatcca
    23281 aaaagttgaa ttactagaaa atgaagatta tgcaagttta ttaaactttg aagcagttca
    23341 agcatttaga gataatgctt tatctccaaa tcatccagtt gctcgtggta ctgctcaaaa
    23401 cccagatata tatttccaaa ctagagaagc ttctaataaa tactatcaaa atatagtagg
    23461 tatagttgaa aaatacatgg aaaaaatgag taatttaaca ggtagaaaac atagtttatt
    23521 tgattactat ggagcagaag atgctaaata cgtattaata gctatgggtt cagtaacaga
    23581 aacaatagaa gaaactatag attacttaaa tgctaaggga gaaaaatatg gtttagttaa
    23641 agttcattta tatagaccat tctcaatgaa acatttctta gatgctatgc catctactgt
    23701 tgaaagaata tgtgtacttg atagaactaa agagccaggt tcaactggtg aaccattata
    23761 cttagatgta cgtgatgtat tctatggaaa agaaaatgct cctatgataa taggtggaag
    23821 atacggatta ggttcaaaag atactactcc ttcagatata aaaactgttt ttgataactt
    23881 agtttcagaa caacctaaaa atggatttac agtaggtata gttgatgatg taactaatac
    23941 ttctttaact ccatctgagc caataaaaat agcttctaaa ggaactataa gatgtaagtt
    24001 ctggggatta ggttctgacg gtactgttgg agctaataaa caagctatca aaataatcgg
    24061 tgaccatact gaaaaatatg ctcaagctta ctttgattat gactctaaaa aatctggtgg
    24121 tataactatg tctcacttaa gatttggtga tactccaata agagctactt atttaataga
    24181 tgaagctgat tatattgcct gtcataaaca atcatatgtt tatcaatatg atttattaaa
    24241 aggtcttaaa aaaggtggta catttgtact taacactata tgggaccaag caggacttga
    24301 agaacattta ccagctcata tgaaacaata tatagctaaa aatgatataa aattctatac
    24361 agttaatgct gttaaactag gacaagaaat aggtcttgga aacagaataa acatgataat
    24421 gcaatctgca ttctttaaat tagctgaaat aataccagaa gaagatgctg ttaaatactt
    24481 aaaagattct atagttaaag cttatggtaa aaaaggtgaa aaaatagtta atatgaacta
    24541 tgctgctgtt gatgcaggaa taaatgcttt agttaaagtt gaagttccag cttcttggga
    24601 aaatgctgtt gatgcagata aaggagaagt taaagaacca gaatttataa aaaatatact
    24661 aagaccaatg actgcacaag aaggaaacaa cttaccagta agtacattca atggtataga
    24721 agatggaaca ttcccttgtg gtactgcagc ttatgaaaaa cgtggtatag ctgttgatgt
    24781 tcctgaatgg ataatagata actgtattca atgtaaccaa tgttctttta tatgtcctca
    24841 tgcatgtata agaccagtat tagttactga agaagaatta gctaatgctc cagaaggatt
    24901 tgaagctaaa aaagcattag gtaaaggatt agaaggatta aaatatagaa ttcaagttag
    24961 tccacttgac tgtacaggat gtggaaactg tgctgacata tgtccagcta aagaaaaagc
    25021 tttagttatg aagccaatag atactcaact tgatacagag ttagataatt gggcatttgc
    25081 agtaaatcct gatgaagtag ctccaaaagt tgatgttatg cctgcaaata ctgttaaagg
    25141 aagtcaattt agacaaccat taatggagtt ttcaggagca tgtgctggat gtggagaaac
    25201 tgcgtacatt aaagtagtta cacaattata tggagataga atgatgatag ctaatgctac
    25261 tggatgttct tctatatggg gagcttctgc accatcaatt ccttatactt gtaaccatga
    25321 aggtaaaggt ccatcttggg caaattcttt attcgaggat aatgctgaat atggatttgg
    25381 tatgtacaca gctgttaagc aaataagaaa taaaatagta gatgctatga cagagttagt
    25441 ttctatggat atctgtgaag atgctaaagc agtatttaca gagtggttag attctagaaa
    25501 tgatggagaa gcttctaaag tagcaagtgc taaggttgtt gaattattag aaaaaccagc
    25561 ttgtgattgt acagatgaaa aagctaaaga attagtaaaa gctataaaag atagaaaaga
    25621 ttatctagtt aagagatctc aatggatact aggtggagac ggttgggcat atgatattgg
    25681 ttacggtgga ttagaccatg ttcttgcatc tggtgaagat gtaaacgtac ttgtatttga
    25741 tacagaagta tattcaaata caggaggtca agcttctaaa gctactccag ctgctgctat
    25801 ggctaagttt gctgcttcag gtaaaaaatc tagaaagaaa gatttaggaa tgatggctat
    25861 gacatatgga aacgtatatg tagctcaagt tgctatgggg gctgataaaa atcaatttat
    25921 caaagccgtt ttagaagctg aaaaacatga tgggccatct ctaataatag cttatgctcc
    25981 atgtataaac catggattaa aagaaggtat gggaagaact gtggctaatg aagcacaagc
    26041 tgttgcttgc ggatactggc atttatacag attcaatcca gaagtaaaag ctgaaggtaa
    26101 aaacccattc actttagatt caaaagaacc aactgcaagc ttcaaagatt tcatacttgc
    26161 acaagtaaga tattctgcta tagctaaaca attcccagag gaagctgatg gattatttgc
    26221 taaagctgaa gctgatgcta aagaaagata tgaaggatac aaaaaattag ctgaataata
    26281 ttaaagtatt ataatgctta tatagaattg ataaaagaga gtgtctcaaa gtgagctttt
    26341 tagttcatga ggcactcttt ttgtatgaaa tcaaatatat tttaatcatt tcaacaatga
    26401 aaaaggcctt atctctattt tgagacagcc tttttttatg aagatattaa gatcctgttt
    26461 ttacctatta aatatctttt taagttttcc attttcatct tgccaatact ttttatatat
    26521 tgttctacac actaaaatta agagaacaaa taatatttat agtatgtata tttggatttg
    26581 ataattttaa aatagataca aaagagtatc tcatttgtgt tttttatcac ttagagatac
    26641 tcttttgata tgcatggaaa aagagtattt tatcctacaa agatttgtgt ccaataaatt
    26701 gaaccaccac ttttttgtgc tactccaaca cctattttac tgaaatttgg attcattata
    26761 ttttttctgt gaccttcaga attcatccat gcgtttacaa cttctttagg agttttttga
    26821 cccatagcta tattttcacc tgctgtctta tagcttattc caaatttttt caacatgtca
    26881 aatggagaac cataagttgg agaattgtgt gaaaaatagt tgttatcaat catatcttga
    26941 gatttttttg tagcaacatt tgatatagaa gaatcaagag taagaggatt taatccagct
    27001 ttagctcttt ctatatttac taagtcgaca acttcttttt ggtaagctga aaaattttca
    27061 cttgtagagc ctgagttgtt attatcatca ggtttgttag tagagtctga attattgttg
    27121 tcttcaggtt tattagactc tggtttattg ttattaggtt tatcagtaga gcctgagtta
    27181 ttgttgtctt caggtttatt agactctggt ttattgttat caggtttatt agtagagcct
    27241 gaattattgt tgtcctcagg tttattagac tctggtttat tgttatcagg cttattagta
    27301 gagtctgaat tattgttgtc ctcaggttta ttagtgttgt tgcaattctt gtcattatga
    27361 ttcttatcct tacaagagtt atctttatca ggtttaagtt ctggttttac agaacaattt
    27421 ggttttgcag aaaacttgta tttagtatta ctatatacta aacttttgtt acttatattt
    27481 acattatttt caagtgatga agcacttgca gtaacagcac ttgaagctgt tgctaaagtt
    27541 aacactccta aagctaatat agtttttaat tttgttggta tatttcttct catcattaaa
    27601 cacctcttaa tctaaaaatt ctaaaaatgt tactgatttg taacctttag tgtagggggt
    27661 acccgtgaat ctatactaac ataagattaa gacttttttt actgtaaaga tatgtaaaaa
    27721 agatatatat ggaataaata ggtatatttc agtgtgtttg aaaatttgta ttaaaagcct
    27781 gtatagtatg gaatta
//
LOCUS       DAELSY010000035        27739 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.035, whole genome shotgun sequence.
ACCESSION   DAELSY010000035 DAELSY010000000
VERSION     DAELSY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27739
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.035"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            73..146
                     /locus_tag="KPG09_001400"
     tRNA            73..146
                     /locus_tag="KPG09_001400"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:105..107,aa:Gly,seq:tcc)
     gene            155..271
                     /gene="rrf"
                     /locus_tag="KPG09_001401"
     rRNA            155..271
                     /gene="rrf"
                     /locus_tag="KPG09_001401"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            545..2563
                     /locus_tag="KPG09_001402"
     CDS             545..2563
                     /locus_tag="KPG09_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0966021.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIK
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
     gene            3076..5088
                     /locus_tag="KPG09_001403"
     CDS             3076..5088
                     /locus_tag="KPG09_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0966022.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNGQDLEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TEDEIEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            5316..7229
                     /locus_tag="KPG09_001404"
     CDS             5316..7229
                     /locus_tag="KPG09_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966023.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTDNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEINTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEENVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLRMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            7222..9441
                     /locus_tag="KPG09_001405"
     CDS             7222..9441
                     /locus_tag="KPG09_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG0966024.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEIALEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     EECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLISENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            9550..10380
                     /locus_tag="KPG09_001406"
     CDS             9550..10380
                     /locus_tag="KPG09_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBG0966025.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYKDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            10370..13837
                     /gene="addB"
                     /locus_tag="KPG09_001407"
     CDS             10370..13837
                     /gene="addB"
                     /locus_tag="KPG09_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBG0966026.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIEN
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDDYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGDNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSTICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            13837..17664
                     /gene="addA"
                     /locus_tag="KPG09_001408"
     CDS             13837..17664
                     /gene="addA"
                     /locus_tag="KPG09_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBG0966027.1"
                     /translation="MSSPKWTKEQLEVIGSRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIANISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDIDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNETESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDSPDSDY
                     YSEIEKKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            17710..18921
                     /locus_tag="KPG09_001409"
     CDS             17710..18921
                     /locus_tag="KPG09_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBG0966028.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVIATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            18908..22435
                     /locus_tag="KPG09_001410"
     CDS             18908..22435
                     /locus_tag="KPG09_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0966029.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNIQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFVVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKDLKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRS
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVINITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            22566..23819
                     /locus_tag="KPG09_001411"
     CDS             22566..23819
                     /locus_tag="KPG09_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG0966030.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            23968..25020
                     /gene="ytvI"
                     /locus_tag="KPG09_001412"
     CDS             23968..25020
                     /gene="ytvI"
                     /locus_tag="KPG09_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG0966031.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            25111..26337
                     /gene="pepT"
                     /locus_tag="KPG09_001413"
     CDS             25111..26337
                     /gene="pepT"
                     /locus_tag="KPG09_001413"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBG0966032.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCREKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            26544..27596
                     /locus_tag="KPG09_001414"
     CDS             26544..27596
                     /locus_tag="KPG09_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBG0966033.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT        11765 a         2722 c         4909 g         8343 t
ORIGIN      
        1 attaggatat atatgtagtt tttagggtgc taactctaaa aaattagttt tactaattta
       61 taaaaattta atgcgggtgt agctcaatgg tagagttctg gccttccaag ccagctgtga
      121 gggttcgatc cccttcaccc gctccaaaag attatgtggt tacaatagca gagaggatac
      181 acctgttccc attccgaaca cagaagttaa gctctctagt gctgatggta cttggtggga
      241 aactgcctgg gagagtagga cgtagccacg taatcttttt ttgttgtatt aaatttaata
      301 aacctacaat tgttatatgt ttttatttgt cttttataga tttttttggc aataaatgat
      361 atagtgaaga taattaaata tattcaatat atttaatttt tttatttttt gtttttataa
      421 tttttttaga agtttatttt ttatgtaatt atttataatt ttgggtaaaa acactttgat
      481 ataatttcaa aaattaaaag gttaaaaaac aaagtagaaa tttatgttaa ataaggagat
      541 taatatgaga agaaatacaa aattattaac aacagggatt ctttcaatgg caatcgtcgc
      601 acctacaatg gcatttgcta ctgaatctaa tgctatggaa aataacgctg atttaaatat
      661 aaacttagag aaaaaaagta tcgttttagg tagcaaatca aaagtcagtg tcaagtttaa
      721 agaaaaacca gatgcagata gcattacatt aaagtataaa tgctatgata tgccattgga
      781 tacaactcta aattataatc aatcaactga gtcatatgaa ggaactatca attataataa
      841 agacccagaa tatttaaatg tttgggaact acaagggata acaataaaca gcaaaaataa
      901 tcctaaaact ttaaacaaac aagaactaga aaagatggga ttaaatttaa aagactataa
      961 tgtaacgcag gaatgtataa ttgaagatat aacttctaga aaagatgtaa ataaatattt
     1021 aagaaaaact tctgcaccta ttacagaact tacaggaagt gatagatacg aaacagcagt
     1081 taaaataagt aaagaaggct ggaaaaatgg ttcagataag gtagttataa taaatgggga
     1141 tgtaagtata gatggcatta tatcaactcc actagcaact acatataatg ctccaatact
     1201 tttggttgaa aaaaataatg tacctaatag tgtaaaatca gaattaaagc gtttaaatcc
     1261 tagagatgta attataattg gagatgagaa tgctatttct aaaactactg ctaatcaaat
     1321 taaatcgact gtaaatgcta gtcaaacacg tctaaagggc tctaatagat atgagacatc
     1381 tttattgata gcaaaggaaa tagataaaaa tcatgatgtg gaaaaagtat acataacaaa
     1441 tgctaatggc ggagaagtgg atgcacttac tatagcagca aaagcaggtc aagacaagca
     1501 accaattata ttaactgata aaaatagtat tacagacaac acatataaat ggttaaagag
     1561 tgaggattta caaaatgctt attttatagg tggacctcaa atgatatcaa caaatgttat
     1621 aaataaggta aatgatataa ctaaagataa tgttactaat aatagagtat atggagcaga
     1681 tagacacgaa acaaatgcaa acgtaataaa aaaattctat acagatgatg agttagaggc
     1741 tgttttagta gctaaatcag atgtacttgt tgatgcttta gcagcaggtc cattggctgc
     1801 gaacttaaaa tctccaatac ttataacacc aaagacgtat gtatctgcat accataaaga
     1861 taatttagaa gctaaatcag ctaataaagt atataaaata ggaggaggat tgacttctaa
     1921 ggtaatgaac tctatagcat catcattatc taaacacaat acaactccaa cagaaccagg
     1981 aaatagtggg ggtaagacag ttatgattga cccaggacat ggtggttcgg acactggaac
     2041 aacaggtaaa ccattagggg gtattaaaga aaaagactat acattaaata cttcacttgc
     2101 gacaactgaa tatttacgtt caaagggatt caatgtaata atgacaagag acacagataa
     2161 gactttatct cttggaaata gaactgcttt atctaactct ttgagaccag atttatttac
     2221 gagtatacac tataatgcat ctgatacaac tgggaatggt gtagaagtat tttataagct
     2281 taaagataaa gatggaggga ctactaaaac tgtagctaca aatatattaa atagaatctt
     2341 agagaaattt aatctaaaaa atagaggtgc aaaaacaaga acactatcaa ctgaccctac
     2401 aaaagattat ttatatgttt taagaaataa tgatatgcca gctgtacttg tagaatgtgc
     2461 atttttggat aatgagaaag atatgagttt acttaataca tcaaataaag taaaagaaat
     2521 gggtacacaa ataggtaaag gcatagaaga ttcattaaaa tagtctacaa tgagagcata
     2581 gaaaaattta aaataaatta atctgcaatt ttaaatttaa actaaagcta taagataaga
     2641 atgtaattat aatagtaata aagactgtat atacattgtg catataagtg cgaaatgagg
     2701 tatatacagt ctttttatga ttaattgtaa aaggatttat attatatgaa gaatttacat
     2761 atcttaaggc tacagttaat tgtcaagagt tggagtattc atttgtaata cacaataaaa
     2821 gcttaattac tatattttat tgtgtgtttg tgtgtttgtg tgttttttgt ggatttttat
     2881 gtcaataaat gatatagtat agataattaa atgtattaaa tatatttaat ttttttattt
     2941 tttgagtttt ataagtttat ttttatatat tacttatata ttatttatat tttgggttag
     3001 atacactttg acatattttc aaaagttaaa agattaaaaa taaagtagaa atttatgtta
     3061 agtaaggaga ttaatatgag aagaaataca aaattattaa caacagggat tctttcaatg
     3121 gcaatcgtcg cacctacaat ggcatttgct actgaatcta atgctatgga aaataacgct
     3181 gatttaaata taaacttaga gaaaaaaagt atcgttttag gtagcaaatc aaaagttagt
     3241 gtcaaattta aagaaaaacc agatgcagat agcattacat taaagtataa atgctatgac
     3301 atgccattgg atacaactct aaattacaat caatcaactg gggcatatga aggaactatc
     3361 aattataacc aagacccaga atatctaaat gtttgggaac tacaagggat aacaataaac
     3421 agcaaaaata atcctaaaac tttaaacgga caagacctag aaaagatggg attaaattta
     3481 aaagactata atgtaacaca ggaatgtata attgaagaca taacttctag aaaagatgta
     3541 aataaatatt tgagaaaaac ttctgcacct attacagaac ttacaggaag tgatagatat
     3601 gaaacagcag ttaaaataag taaagagggc tggaaaaatg gttcagataa agtagttata
     3661 ataaatggag atgtaagtat agatggcatt atatcaactc cgctggcaac cacatataat
     3721 gctccaatac ttttggttga aaaaaacaat gtgcctaata gtgtaaaatc agaattaaag
     3781 cgcctaaacc ctaaagatat aattataatt ggagatgaga atgctatttc taaaactact
     3841 gctaatcaaa ttaaatcgac tgtaaatgct agtcaaacac gtttaaatgg ttctaataga
     3901 tatgagacat ctttattgat agcaaaggaa atagataaaa atcatgatgt ggaaaaagta
     3961 tacataacaa atgctaatgg cggagaagtg gatgcactta ctatagcagc aaaagcaggt
     4021 caagacaagc aaccaattat attaactgat aaagatagta ttacagacaa tacatataaa
     4081 tggttaaaga gtgaggattt acaaaatgct tattttatag gtggtcctca aatgatatca
     4141 acaaatgtta taaataaggt aaatggaata actaaggata gtgttactaa taatagagta
     4201 tacggagcag atagacacga aacaaatgca aatgtaataa aaaaattcta tacagaggat
     4261 gagatagagg ctgttttagt agctaaatca gatgtacttg ttgatgcttt agcagcaggt
     4321 ccattggctg cgaacttaaa atctccaata cttataacac caaagacgta tgtatctgca
     4381 taccataaag ataatttaga agctaaatca gctaataaag tatacaaaat aggtggagga
     4441 ttgacttcta aggtaatgag ctctatagca tcatcattat ctaaacacaa tacgactcca
     4501 acagaaccag gaaatagtgg gggcaagaca gttatgattg acccagggca tggtggttca
     4561 gcacctggaa attcatctgg aggaatgatt gaaaaagatt acaatttaaa tacttcactt
     4621 gcaacaactg aatatttacg ttcaaaggga ttcaatgtaa taatgacaag agacacagat
     4681 aagactttat ctcttggaaa tagaactgct ctatctaatt cattgaaacc agatttattt
     4741 acaagtatac attataatgg ctcaactaat aaacaaggtc atggtgtaga agtattttat
     4801 aagcttaaag ataaaaatgg ggggactact aaaactgtag ctaccaatat attaaataga
     4861 attttagaga aatttaaact tacaaataga ggtataaaaa caagagtact tcctagtgat
     4921 tctacaaaag attatttata cgttttaaga agtaatgata tgccagctgt acttgtagaa
     4981 tgtgcatttt tggataatga aaatgatatg agtttaataa actcatctgc aaaagtaaaa
     5041 gaaatgggta cacaaatagg taaaggaata gaagattcat taaaataaat ggaaataaaa
     5101 atatttaaaa acttaatgta aatttatact aaagtcaaaa aatgaaatat aagtgtatca
     5161 atagtaagac tgtatgtaca tttgcacatg tgaatgcaag tatatataca gtcttttttt
     5221 gtagttaaat gcaataattt gattgtgttt tgtaaaatac acaatatgat ataattagat
     5281 aatatacact taatttaatt gaggaaggtg attatatgga atatttattt gaaaaaagta
     5341 gtttaaaaga atatggaaat gaagctttag ataaatatag agaattatta gataaaactg
     5401 ataataattc aaggtgtatt cttttattag taccaaacaa taacacaaag atacgatatg
     5461 aaagggctct aagattaaat tatagtgaag aactgaaaat aacaacatat ataagttttg
     5521 tgaagaaaga attagttaaa tactggccac taataataga aaaatgtgat gaaataaata
     5581 caaaggttgt atctccaaca tttatatcaa atagtttaag tgaatattta atagtgcaaa
     5641 aagttaagga aaaaaggata caagaaggat attttgaaga tattacagga accaataaat
     5701 ccatagcaaa tagtataatg actaacataa ataaatctgc atttaattta attgattttg
     5761 attttattgg agaaaaaata tattcatcaa agaaaaataa agatagtata tatagatttt
     5821 catacactca gatggatgaa attatatcat attatataaa ttcgttacta tcaaattcaa
     5881 ttttagataa tgcattaagc atatatttat acaatcaata cctacttaat gatgattttt
     5941 atttaaaaaa cttatttaca gaagtgagat atataattgt ggatagtctt gaaagctcaa
     6001 gtaatgcaga agttgatttt ataacagaag cattggataa tacattacaa agttatatat
     6061 attttgatta tagtagggat tattctgtat ttaataatgt ggatatagac tatataaatg
     6121 agaaaataat atcaaaaatt aagcctaaag aagaaaatat taaaaagaag gagattagaa
     6181 tagaagattt atacttatta cctgcaaata tagaattaaa tcaatcaagt caattataca
     6241 atgaaatgtt agatttaata agtgaaaaag ttatatctct tatagaagaa aatgtatctc
     6301 caagagatat agcaattata tctcctataa acaatagtat attggagcat caaattagag
     6361 attctttgat agaaaaaaat atagatgttt ttagtactaa gaaggataaa aaagctgtag
     6421 attatccata tgggaatgca ttggtagtgg ctacatgtat attttatgga tatttagatt
     6481 ttataaaaga tgatgaatac ttaagctttt tagaaactct tttagaaata aataggataa
     6541 aagcatttaa gatttttaat gaaacaagac atttaagtat tgataatata gatgaacata
     6601 gtcagtatcg tgatatttta cagtatattg aagaaaaaaa gaagagtgat ataaaaatac
     6661 atgaattttt aactcaattt tacatagata aaatgttaaa tttaaaagaa ggtaaaaaga
     6721 atgttgggtt atgtaaaaag ataatagctg aaagtgagtc ttttagtgaa agtttaagtc
     6781 tacttaggat gaaaaaagaa aagatattta tagaagcttt aaaaagtaca ataaacgatt
     6841 attactctgt tgtcgatata gaagagttga aatccaaaga caaaatagta ataactactc
     6901 cttattcata tatatcatca aatatagata gaagtataca aatatgggta gatataggaa
     6961 gtaatgcttg gaatatgaaa atagaaaaag atataagtaa tttaattgta cttagaaaat
     7021 cttttgaaga aaaaaagatt tatacaaatg aaatggaaga atattataaa aaatattatc
     7081 tatacaatac agtatataat ttgttattaa atgcaaagaa aatctatgca tttaaaagtg
     7141 aatatgcgat taatggttat atccaagaga gtatattata tagtatactt ttaaaaatct
     7201 cacataaagg agataaaaat tatgattaat ataaattata gagaagacca gataccaata
     7261 ataaattacg ataatggtac tatggcagta ccagcagtac caggtgctgg aaaaacattt
     7321 ataataacaa atttagtagc taaactatta cttgaacaga aacataaggg tggtaaaata
     7381 ttaattctta catatatgaa tagtgctgta aataatttta aaggtagaat aagaaagatt
     7441 cttgaagaga ataaaataga tgatactaat tcgtatgaag taatgactat acacagtttg
     7501 gctgtaaaaa taataaagga aaagccagaa gttgtaatgt taagtgagga ttttaacata
     7561 gctgacgatt tgcaaaaaag tattatatta aatgaatgta taagtagatt tagatttgaa
     7621 ggtggagaaa gagcttttag atggttttta aaggagcaaa aagatgaacg ctggaaagaa
     7681 atagctcttg aagcctggga aagaggtttt tttgaatttg caggaaatgc aataagtgaa
     7741 cttaagtata agggaatttc tccagataaa cttgaagaaa tattgtcaag aggacacaaa
     7801 ggaatgctta aaattatttt accaatatat aaattgtatg atagaaaatt aaagcaaaat
     7861 ggattgctag attatgatga tatattaatt ttagcagaaa aaactttaag cttagacgaa
     7921 gggcttagaa agaagtttca aactagatac aaatatatat ttgaagatga gtgtcaggac
     7981 tcaaatgaaa ttcaaggtaa tataataaaa attatatcta gtgaaaataa aaacctagtt
     8041 agagttggag atataaatca gagtataaca ggaacatttt catcatcaga ccctaaattt
     8101 tttaaagaat ttataaatag tgcagatttc tgttatagga tggatatgtc aaatcgaagt
     8161 tcaaaagata tacttgattt agcaaacact cttgtcaagt atgttacaaa ggaatttaga
     8221 caagaagaat gtagacatgc tcttgaagat atgcaaataa aaactgtgcc aaatggaatg
     8281 ggatataaag aaaatcctaa tccagaacat tataatataa atgtaaagtg gtatgagagt
     8341 tggaaaattg aaatagagca aactgctaga tacgttaagg gtataaaaaa taaatatcca
     8401 gataaaagta taggtatatt ggttccattt aatgaacaag tgacacaagt tgctaaagaa
     8461 cttagagaga ataatctaac ctttgaagaa ttaggaccaa attctttgaa taaaaggaag
     8521 gttataaaca acatagcata tattattgat tttatgttac attgtgatga tgtagaaaag
     8581 ttaatagttg tacttaataa agtttttata gatactgata atgaagaaga aataaagtat
     8641 tttttagaag aattaaaaaa ctattctacc gaagaaatta tttataattt tgaatttagt
     8701 aagttagata atgatttaat agaatttaaa gaagaaagtg acatatattt aagttttaaa
     8761 agaggaatag atattataaa acagatatta gaatatccaa taataaggtt agacctctta
     8821 gttttgttta ttgggaaaaa acttaattta gaaaaagaag ataaagctgt actggattat
     8881 atatcttttt atataaagta tctaatatca gaaaatataa atatggattt agaagatgta
     8941 tataatttat tgtttgatgt aaagaatagg gtgtttaatc atataataga tgtggtatat
     9001 gagataaatg gatatgaacc agagcccggt agtataacac tatgtaacta tcataagtca
     9061 aaggggatgg aatgggattg tgtgttttta ctaggtcttg ttgaatataa tttccctgac
     9121 aatattaatc aaaagtttca gagtgataaa tggtatctaa aagaaaaata taaaaaccct
     9181 atggcaatta ttaaatcaga agttgaagct atattaaaag gaagtatttc aacagattat
     9241 gctcataaaa cgaagattga ttctataaat gaaaagatta gacttttata tgtagggatt
     9301 actagagcaa aggaaatgtt agtactttca ggaagtgctt atagagatga aagtgatata
     9361 gggaataaga gaaaagagca aaaaccatgt atatatttaa gcagacttaa tcaacatatt
     9421 attgaaaaaa ggtcaaatta atatataata agttattaaa agattgcttg aaatatatct
     9481 aaaaggggat aaaacaatat gtacagtaag taactactaa aaaataaagt taagagagaa
     9541 gtgatatgag tgaacgataa attaaaatat ttttcttata gtcaaaattc aatcaacaca
     9601 tataaatcat gtccattgaa atttaaatat aaatatattg atagaataaa ttggaaaaat
     9661 gatgatgttg gtagtagaga gtattatgaa actttaaaaa caggaagaga ttttcatttg
     9721 atttgtgaaa gatattttag caatatacct cttggaatct attttaatga ggatgataaa
     9781 aatagtaaaa agtttttaaa gtggatggaa aatataaaaa aagttgttcc tattgaaaaa
     9841 ggaaaaacgt atttgccaga atatgaagta aggatgacct taaatgggga tataatacag
     9901 gcaaagtatg atttagttgt aattggaaat aacaatatag aaatatggga ttggaaaacc
     9961 gagtctaaaa agattgacta taaacacgta gaaaatagaa tacaaactat tgtttatatg
    10021 tttttagcaa aagaagtaat acctaaagtt ttaaaaatgg acataaatgt taaagatata
    10081 aatatgagat attatcaacc tgaatttgat gacttaccaa ttaccatttt atatgatgaa
    10141 gaaaaacatg aagcaaatag aaataaaatt caaaattata taagtatgat aaaaaacact
    10201 aattatgaag aacatacata caaagacaat ctatacaatg atatagaaaa ggtatataat
    10261 aaagaagaac gtatgtgcta tagaaataaa aagcattgca agtattgtga atttaataag
    10321 ttatgtaatg gcaaagaaat agattatagt attttggagg cggaaattta tgggacttag
    10381 gtttgtatta ggtagaagtg gaagtggaaa atccacctat atactagatg agattaaaaa
    10441 agaagctcaa aaaaatgaaa cgacatcaat tatactttta gttccagaac agtacacatt
    10501 tgaggctgaa aatagagtaa gtaagctatt cttaggaaaa gaaaaagata agtatctaag
    10561 agttagggta cttagcttta agacactaag taatatagtt ttttctcaag taggaggatt
    10621 gacagatgta aatataaatt ctagtggaaa agcaatgatg gtatatagag ctattgaaga
    10681 tgtaagtgaa gaattaaatg tgttttcaaa atcaaaatcg cagtctggtt ttgtaagctc
    10741 aattacagat atgatatcag aaatgaaaca atacaatata agccctgaaa tgttagaaaa
    10801 tatatctgga gaattggata atgaaacttt aagtttaaaa ttaaaagata taagtaaaat
    10861 atataattct tttgaaggga aattgcatga aaattatgta gatgctcaag atatgttaac
    10921 ttcattggca agtaaaattg aattaagcag ttatttagat ggagcttgtg tttatataga
    10981 tgaatttaca ggttttacac caaatcagta caatgtcata aagagtatat taaataagtc
    11041 taaaagtgtt aatatctctt tgactgtaga tgatatcaac tatattgggt acagtaaatc
    11101 tgatatgttt tctagaacga aatttactta ttcaaaattg actcaattgt gcaacgaaga
    11161 aggcataaaa atattgccac aagtaaattt aaacacaggt gttataaaaa gatttgaaaa
    11221 agttaaggaa ttacagcacc tagaaagatt ttataatgct tatccatata aaatatattc
    11281 aaatccaact gaaaatataa agataaaaga gtttaacaat ttatattcag aggtggaaga
    11341 gatagccaga gaaattgtac atttagttag agataagaat gtaagatata gagatataac
    11401 cattgctaca agagatttaa atagatatga ttttttagta cactctatat ttaatgaata
    11461 taatatacca aactttatag ataagaagag agaggcaaaa agtaatccaa tagttatttt
    11521 aattatttca gctttagaaa tgaagaatag acgatatggg tatgaaacta tgtttaggta
    11581 tttaaaaagt ggacttatag gaattgataa tgatgatata aatttactag aaaattatgt
    11641 gttggctaat gggattaaag gtaaaaaatg gtttgatgaa aaatgggatt atagaataac
    11701 acaaagtctt tcaggtcaag aaagtgaatt tgaactggaa ttaaaagaaa aaataaatga
    11761 aataaaaaat agagtattag agcctatagt aattttacaa gaaaaattaa gaggaaaaaa
    11821 tagagtaaaa gaaatctgta gatatatata tgaattttta ttagatataa atatgccaga
    11881 aactatagaa aatttaatag taaattttaa ggataaaggg gaacttgatg ttgcaaatca
    11941 atactcacaa gtttgggaca tagtagtgga catattagac caaatggtgg aacttatggg
    12001 tgatgaaata atttctttag aaaaatttat aaaattaata accttaggct ttgatgaata
    12061 tgaattagga ctggttcctc ctagtataga ccaggtactt gtaagtagtg tggatagaat
    12121 gaaaaatcca gacacgaaat atttatacct agttggaact actgatggtg tgtttccact
    12181 gattacaaag gatagtggaa tattgagtga taatgatagg gaaagtcttg gaaacaaagg
    12241 aatcgaagtt gatattgata gtaagactag gagttttgaa gaacagtttc ttgtatataa
    12301 agctcttact tcgacatcta agaatttaac cataacatat ccaatatcag accatgaagg
    12361 taaaacgctt agaccatcaa taattatatc aagacttaag aagatatttc caaatataga
    12421 aaataaaagt tatttagtgg aagaaaataa aagcactgat aaagatatac ttaaaaaaat
    12481 aacggttaaa tcccctacat ttaatgaact tataaacgta ataaaaaatt atgacagtga
    12541 tgattacaat acagaagaga ttaatagtat ttggttagat atatataggt actatttaaa
    12601 agatgaaatc tatagttcta taacaaaaaa agttataaaa ggtcttagtt atactaatca
    12661 agttcataag attgaagaga aaaaaataag gtcattgtat aaaagtaatt ctcttagcat
    12721 atctaggctt gaaaaatatg ctgaatgtcc atttgcttat tttatacaat atggtctaaa
    12781 agcaaaaaag cgtaaagagt attcatttac accaccagat ttaggaactt ttattcataa
    12841 tattttagat aggttttcaa aagaactttt acaagataat ttaacttgga gagatattga
    12901 tgaaaaatat atagaattaa agataggtat aatagttgat gaaataattt taaagatacc
    12961 aggatatata ttaaatagtt ctgagaggta taaatacctt gcatatagat taaagaatat
    13021 gttgacaact gctataacta taattagtca gcaaataaaa caaggttcat ttgaaccaat
    13081 agattatgaa gttaaatttg gcgacaatgg aaaatatcca cctataaaaa tggtgttaga
    13141 aaatggacaa gaagtaagtt taattggaca aatagataga gttgatgaat ttgaagaagg
    13201 agaaaataaa tatataagaa taatagatta taagtcaggc aataagtcca taagtctaac
    13261 agaaatatat tatggtctac aattacaatt acttgtgtat ttagatgcaa tccttgaaag
    13321 tgcaaaggat gaagatataa atataaatcc agctgcaata ctatattgta gaataaataa
    13381 tccaatagca aaatttaatg aagataaaga tgatgaagaa atacaagaag caatattaaa
    13441 agaacttaga atgaaggggc ttgtagtaaa agactctcac atagttaaag agatggataa
    13501 atcattaata gatggagaaa ggaaaaactc tttagtaata ccagtaggtc ttactaagga
    13561 tggaaatgta ggtaaaagta cttcagcaat aagctatgag gatttcaaac tgcttagaaa
    13621 atatgtaagg catgctataa aggatttatg tgaagaaatg ttaagtggag aaatacgtat
    13681 agctccatat aaacataaag atggaacatc atgtgacttt tgtgattact ctactatatg
    13741 tcagtttgat tcaactataa aagataacaa gtataaaaat ttaaataata agagtaatga
    13801 agagataata aaaatgatga aaggagatgt taattagtga gttctcctaa atggacaaaa
    13861 gagcaattag aagttatagg aagtagagaa tgcaatttat tggttgctgc tgctgctggt
    13921 tcaggaaaaa cagccgttct agttgagcgt ataattcaaa tgataacaag tagagaaaat
    13981 ccaattgata tagataagtt attagttgtt acatttacta atgctgctgc gtcagaaatg
    14041 agagagagga taggggatgc tataggaaaa gccttagatg aaaacccaga aaataaacat
    14101 ttacaaaacc aacttgtttt attaaataaa tctagtatta caacaataca ctcattctgt
    14161 ttggatgtta taaaatcaaa ctttcataga ataaacttag accctaattt cagaatagga
    14221 gaccaaactg aatgtgctat tttgaaacaa gaagcaatag aggaagtatt tgaagattta
    14281 tatgaagaaa gagatgaggg gtttttaaat ttagttgaaa gttatgcaga aagaggagga
    14341 gataaagaag tacaagatat tatcttggga atatattcat ttgcaatggc ttctcctgaa
    14401 cctaaaaaat ggttgattga ctcagcagaa cgatttaata ttgatgaaaa ttttgatttt
    14461 tcacagtcta tttgggctag agctatatta gacactgtaa aaatagaaat taatggtctt
    14521 tgtctaaata tggaaagggc attaaaagaa gtggaatcaa tagaagaatt agaaactttt
    14581 gcagaaaagt tatccgttga atataaaaaa attgctaaca tttcacaagc atgtaataaa
    14641 tcttgggatg aggcatataa aaagatggca agtatgtcct ttgaaaatta tgtaaaagga
    14701 gtaaaaagaa tttcaaaaga tgctccttcg tatataaaag aatcaaaaga aaaggcaaaa
    14761 actataagag ataagactaa gaagtcttta gaaagtatag taagtgctac attcaataaa
    14821 gataatgatt ctattagaga agaaataaaa tatctttata acatagttaa accaatatct
    14881 agtgttgtac tgagatttga agaggaatat agcaataaga aaagagaaaa aggaataata
    14941 gattttaacg atatagagca ttttgcatta aatatactta cagatataga tgaaaaaggt
    15001 aatattgtac cttctgatat agccgttgga tatagaaaca agttttatga aatatttata
    15061 gatgaatatc aggatagtaa tctagtgcag gaagtactgc ttaaagctgt tgcaaataca
    15121 gaaactccaa atagatttat ggttggtgat gtaaaacaga gtatatatag atttagacaa
    15181 gcaaagccag aattattttt gcaaaaatat aataactata atgataaaaa gggaagttcc
    15241 catagaaaaa taatgcttta caaaaatttt agaagtagag aagaagttgt agatgctgta
    15301 aattatatat ttgaaaatat aatgaatgag aatataggag aaattgaata tacagaaaaa
    15361 gaaagactaa atttaggtgc gaattttaat gtggatacag acgagaaatc tattattggt
    15421 ggagcaacgg agatacacct aatacaaaaa gataataaac ttgatgatga tattataaat
    15481 gataaagacg atagaattaa taacaaagaa aatgaaatag aggaagaaga aaaattagat
    15541 aatatacaac ttgaggctag aatggttgga aacattatca aagatttaat gaaagtcaat
    15601 gaagatggaa aaattcaaaa ggtctatgat aagggaatag atggatatag acctgtggaa
    15661 tttagagata tagttattct tttaagagct acatctgcat gggctccagt gtttgcagat
    15721 gaattgatga atatggatat accaacatat gctgatgttg gagtggggta ttttgatacg
    15781 attgaaataa aaacaatatt gtcattactt caaataatag ataatccaat gcaagatata
    15841 cctcttatat ctgtattaaa atcacctata tttggattta caccagagga tttgattgat
    15901 attagagttc agagtaaaga taaaattttt tatgaggttt taaaaagtac agcagagtat
    15961 gatggattta cagattctca aaatgaaact gagtctgagt ttataccaag tgaagagtgc
    16021 ataaacaaaa gtaaagattt tttaattaag ctaaaggaat ttaaagaaaa gtcaatgtac
    16081 atgagtacag atgaatttat atggtacttg tacacaagga ctggatacta tgcttatgtt
    16141 ggagcattgc caggtggaag tcaaagacaa gctaatttaa aagtattgtt tgagagagca
    16201 aaacaatttg aagaaaccag tcttaaagga atattcaact ttgtaaattt tatagagaaa
    16261 ttaaaaaaat ctagttctga tatgggaagt gcaaaaacac ttggtgagaa tgcaaatgtt
    16321 gttagaataa tgagtattca taaaagtaaa ggtctagaat ttccagtagt catatgctct
    16381 gcaatgggta aaaatttcaa tactcaagat tttaaaaaga gtattttata tcatcataat
    16441 ttagggtatg gacctcaatt tgtagactat gagagaagga tttcttttcc aagtatagcc
    16501 aaagaagcat taaaaagcaa aataaatata gaaaatttat cagaagagat gagagtttta
    16561 tatgttgcat ttacaagagc taaagaaaaa ttgattataa ctggttctac tagaaatata
    16621 caagatagta taaagagatg gtcaaatgga attgaaagtt tagatacaat atcacaatac
    16681 gagatattaa aaggtaaaaa ttttttagat tggataatgc cttgtgtatt aagacatagg
    16741 gatttatcta atctactaga agaagtagga ttagacgcag tttttaatgt tgaacataat
    16801 tcaaaatggt atggaaagtt gtggaataag aatgatattt tagtggaaaa gaagagtgat
    16861 gaagaaaaag aaagtattga agaaattctt gaaaaaatag atgtagatag tccggacagt
    16921 gattattaca gtgaaataga aaaaaaatta aactatatat atccatatga atttagtaca
    16981 agaaaacctg ctactatatc tgttacagaa ataaaaaaga tacaaaataa ttatgaagaa
    17041 gagttaataa atactatatt tgaacaaaaa gtaatattaa aaaagcctct atttattcaa
    17101 aatgaagaag aaagagaaaa aataagtgga acagaaagag gtacaatagt ccatttggtt
    17161 atggaggttt tagacttaaa aaatgtaagt agtgtaaatg atataaaatc acaaataaga
    17221 ggttttgttt caaaaggtat aataacagaa aaacaagcta gtatagtaaa tccatataaa
    17281 atatataaat tttttgcctc taacatagga aaaagaatgt tgaatgctga aattataaat
    17341 agagaaaaat caatttatgc acaagtaaat atgaaagaca tatatattta tgaaaagctt
    17401 ataaacaatg atgataaaaa actttatgat aatgaaagtg tcatgttaag aggtatagtt
    17461 gatgcttatt ttgaagaaga taatcaaata gttttggtgg attataaaac agattttgta
    17521 aatgaggaaa atataaatca aataatagag aaatataaaa aacagttgga tttatatgca
    17581 gatattattg aaactttaac tggtaagtca gtaaaagaga aatgtatata tttatttgga
    17641 gttgatgaag ctgtttgtta ttagttttta tatataaatt aaacctaaaa gatagaggaa
    17701 ggggaaaaaa tgagatttat tcatacatca gattggcatc tagggaagtc cctagaaggc
    17761 cattctagaa ttgaggaaca agcaaagttt tgtgaggaat ttataaaaat agtagaatca
    17821 aatgaaatag atatggttat aattgctgga gatgtatatg atacttctaa tcccccagca
    17881 caagcagaaa aactatttta tcagactgta tcaagacttg ccaataatgg tcagaggtgt
    17941 gtattaataa tttcaggaaa tcatgataat ccagagagac tttctgcaat aacacctcta
    18001 gcacatgaac aggggatact tatatatggt tatccattaa gtgctacgat agaagctaag
    18061 tataaagggt ttgaaataac atatgcaact cagggatgta caaaactgaa tataaatgga
    18121 gaaaatattg tgattgctac attaccttat ccaagtgaaa aaagattaaa tgaagttttt
    18181 tcaagtgaag atgagtttga gaaacaaaaa aattattctg aaaaggtagg agatatattt
    18241 agaagtttag aagaaaattt tagaagtgat actataaata tagccgtatc acatatattt
    18301 gtaataggtg gagagagtac tgaatcagaa agacctatac aattaggtgg aagtttttta
    18361 gttgaaagaa aagatttgcc agaaaaggct caatatactg cactaggtca tttacataaa
    18421 caacaaaaag cttccgaacg tttaaatgca tactattctg gttctccact tcaatatagc
    18481 aaggatgaaa gagcgtatac caaaggggca tacatagtgg atataaaagc tggagaaaaa
    18541 ccaataattg aagatgtata ttttaataat tacaaaccta tagaagtatt taaatgtaat
    18601 ggaatagaag aagcactaga tatatgtgag caaaatcaag atagagagat ttggtcatat
    18661 tttgaaataa atactgatga gataatatca cagaatgaaa ttaaaaaaat gaaggaacta
    18721 ttaaaagaca taatagaaat aaaaccaata ataacatctt gttatgaaca agaaagtgta
    18781 gatataaaag aaaagtctat ggcagagttg tttagagagt tctattcttt tagtaaaggt
    18841 gtagagccaa aaggagaact tatggattta tttctagata taataagtga agaaggtgaa
    18901 tcagcagatg agaccaatta gattagaact tacaggactt aatagctata ttgataaaca
    18961 agttatagac tttgaaaaat taattgaaag aggtttgttt gggatatttg gaactactgg
    19021 aagtggaaaa tctacaatct tagatgcaat aactattgct atgtatggaa atatatccag
    19081 aaatacaaaa gaatatataa acagtgtgtg cgataaggca attatatcat acgagtttga
    19141 gataggaagc aaaaacacta gaagaagata cattgtagat agaaccattg ctagaagcaa
    19201 aacaggaaca aagacctcta atgctagatt aatagaagta ttgaatgaca atatccaaaa
    19261 tgttttagct gataaagttg tggaagtaaa tgaaaaagta gcacaggttg taggattaac
    19321 ggcaaatgat tttactaggt cagtagtttt accacaagac aaatttaatg agtttctaag
    19381 attatcacgt gcagatagaa gagacatgtt agagagaatt tttaatcttg agaaatatgg
    19441 aagaagtctt ggagaaaaag tgaaaaagag aaaaaatata cagttacaaa atttaaaaga
    19501 tttgaaatca aaattaagtc aatatgatgg aattacagag gaagtataca acaatataaa
    19561 ccaagaatta atagagttaa aaaatttgga aaaagataag aataatgctt tagacctagc
    19621 tcaaaaatct tatgaagaaa gtaaaacagt atatgaagaa caattaaagt tagaaaaaaa
    19681 tgaacttaga aaaaatgagc ttgatttaaa aaatagtgag ataaaagaaa agattgctct
    19741 tgtagaaaat gatagtaacg ctaaaaaggt agacccatac ataagttcag ttcaaaatct
    19801 agaaaaaaag atagatgaag atagctttgt agtaagtaat ttagaaaaga aattagcaat
    19861 attaaatcag gagcttgaag ttacaaaaaa tagatatgaa aaaataaaca aaattaagaa
    19921 tgaagaagtc cctaaattga gtgaagaaaa aataagattg caacaagcaa taaaattaga
    19981 agaagagtta gttttattag atagagagtt aaaagatcta aaagaaagtg gaattaattt
    20041 aaataagact aaagttgaat tagaaaaagt aaagcaggtg tcagagtcca gaaaagatgc
    20101 agttactaaa tctataaaag aagttgaagg aaagattgat aaagtaaata taagtgcaga
    20161 attaaaacaa aaaatatttc tagcatatga atacgagaaa gattacaaca acgtattgga
    20221 agaaaaaaat caaaagctaa ataaactaga agaaatactc aaagagacag aaaatataaa
    20281 tttaaaagtt agatatattg ataaagataa gaatgatgta aatcgtaatt tagaaaattt
    20341 atcacttcac ttggatgttc ttcttaaaaa atgtcctggt aagagtgctg atttactttt
    20401 aaaatctgaa tatgttactg aattaagaag taaagcaaat aatactaaag aaaatgaaat
    20461 taagaaaagc agtatacaag atgaattaaa gataatactt gaaagtaagt ttaatactga
    20521 aagggaagta aatttactaa atgaaaaact tgaaaataat agaaagaata gagatgacct
    20581 agaaaaagaa ttagaagaac taaagtattt aaatttagcc tctgaactta gacgtgaatt
    20641 aaaagaaaat atgccttgtc cagtttgtgg gtcaaaacat catgaaaatc ataatataac
    20701 taagtatgat gaaaatatat cttttgtcaa agaaaaactg gagaaattag aaaaagaaaa
    20761 aataagtata agaaacaata tagaggaatt aaatgctaaa gtaagtgggt atttatctat
    20821 agaaaaaatg aagactaaag aacttgaaga tgtaaaagga aaactagggg ggattccatc
    20881 tagtcaatta ttaaaaaaac ttgatgaaga acaaagaaaa cttgcattgt taaaatctaa
    20941 tattcaggag tgggagaaag aaaaagagag tactgaaaat aaagtaacat tagctaaaga
    21001 agaaaaaaat aaaattgaaa aagaagaatt aaaaataaga gagagtctga ataattataa
    21061 gaaattaaca agagatttaa acatagaaat agaaggttta gaaaataaat gtaaaaaact
    21121 taaacaggaa tacctaggat taaagacgat tactaaagtc tctaatctat tgtcaaaagt
    21181 aaaagaaata agggagaatg aaaaagaatt agaattacta agtactgggc attcaaattt
    21241 attaaaaaat agagactcac tagatattaa aataagagaa aatgagagta aactacatga
    21301 aatagagata gaattaataa aggctagaga attatatgta gaaaagaaat tgagtagaga
    21361 taataagtat aaagaagtta ttaacataac gaaaggtgat ttagctaaaa atcttttaca
    21421 taatgttgaa gaaaatatat gtaagatatt agaacaagaa gaatcaagta agaaaaaatt
    21481 ggaagagcaa agattagaat atgaaaaaaa cattgctgaa aagcacaata tagatggtag
    21541 acttaaaact gctaaggaac aatataaaga ccaaaaatca acactaaata aactattagc
    21601 agaaaataaa tttgaaagta tatatgcagt aaaaagagct ttattagata ctgatgcagt
    21661 aaaaaaattg attgaagaaa tactagagca tgaagaagaa caaaagttat tatcatttaa
    21721 gataaaatca tcaaaagaaa aattaaatgg aagaagcata aagaaggagt actttgaaca
    21781 gttaaaagat gaaatttata atttgaaagt agaaatagga aatatatcca aagatattgg
    21841 agctaatcaa aatcagttaa taacactaaa agactcttta gataagataa atgattttaa
    21901 taaacaactt aaggtagttg aacacaatgt ggacttattg gaagaattag ataaatgtgt
    21961 gcaaggaaat aaatttgtag aatatgtttc tacaaaccaa ttaaaatata tagcattgga
    22021 agcatcaaaa cgattggatg gaataacaaa aggaagatat gcacttgaaa ttgactcaac
    22081 actaaacttt gtaatgagag ataactttaa tggaggagaa agaagaagca ttgatactct
    22141 atctggtgga gagacattct tgacatctct atcattagca cttgctcttt catctcaaat
    22201 acaattaaag ggaagtgcac cattagaatt tttcttttta gatgaaggtt ttggttcatt
    22261 agatagtgaa cttttagaaa ttgttataga atccttggaa agattgcata gtaataatct
    22321 aagtgtgggt ataataagtc atgtcgagga acttaaaaat agagttcctg ttaagctatt
    22381 agtttcatct agtgaggctg gaataggttc aaaagtaaaa attgaatata gttagaaaat
    22441 atatggtaat tgaatatatt atctaatatt tccaaataat atgtaattgt gatataataa
    22501 aaactaatat taaagttatt tataattaat attaatataa aaacatgtaa gtaggagtgt
    22561 ggtaagtgtt ggaatcgccc aataatttga ttcagattat ttttttaata gtattactta
    22621 tagggtctgc atttttctca gcatctgaaa cggccttaat gtcgttgagt aaaatcagaa
    22681 tcagatatat gcaagatgaa ggagttaaag gagctaagtt agtaagttca ttaatagaaa
    22741 atccaaataa gcttttaagt tctatattgg ttggaaataa cgtcgtaaat atagcagcaa
    22801 catccatatc aacatctttg tttataggat taatgggaga aaaaggagtg gcacttgcta
    22861 cagctgttat gacagtatta gtattaatat ttggtgagat tactccaaaa acaatagcag
    22921 caaataattc agaaaaggtt tctctattag tatctaaacc tataaaagca attatattta
    22981 tattaagacc aatagtatgg atatttaata taattacaaa tattatattt aaattatttg
    23041 gtataacaaa taaaggagct aaatcattca taactgaaga agagctaaaa actatggtaa
    23101 atgtaagtca tgaagaaggc gtccttgaaa tggaagagag agaaatcata aataatgttt
    23161 ttgaatttgg agatatgcaa gctaaaaatg caatggtaca aagaatagat atggtagcta
    23221 tagatatgga agatagttat gatgagataa tacaggtatt taaaactgaa aaattaagta
    23281 gaatgcctgt gtatgaagaa actatagatg atattgtagg aatacttaat ataaaagata
    23341 ttatattttt atctgatgaa gaaatagagt catttgatat taaaaattat atgagagaac
    23401 cttttttcac gtatgagttc aaaaagataa cacaacttct tgaagagatg aaacttgaaa
    23461 aaagtcagat ggctatagtt gtagatgagt atggtggaac atctggactt ttaactatag
    23521 aagatttagt agaagtcatt gttggagata tagaggatga atatgatgaa gaagaggatg
    23581 aaatacaagt tataaaagaa gatgagtata ttgtagatgg aagtactaaa attggtgatg
    23641 ttaatgaact tattggtgta aacttggaat ctgaggagtt tgattccata ggaggtttta
    23701 taattggaca cctaagcaga ttgccagaag aaaatgaagt aattgaagtt gataatataa
    23761 gattttgtat agaaagtata gaaaaaaata gaattaaaaa aataagaata tatacataat
    23821 gtaataatgt tcttaaattt attagaagtg tataaatata aaagatatct tttatattta
    23881 tacacttctt tttattttgt attcaaataa aactagtatg aaatggacaa aataaaaata
    23941 taaattagaa gaaaggagat ttttaagatg agcatactag aaaataattt tttatacaaa
    24001 ttaaaaaaca acactatctt tttttgtata tatacattgt tatttatttt tatttacaaa
    24061 gcgtttccat acatagctcc attttttcta ggtgctataa tagcacttat gattaatcct
    24121 atatcacaaa agttagaaaa taagtttcgt ataaataaag gtatctctac tcttgttcta
    24181 agttttcttg cagtggctat agtcagtaca gttactacaa taatagtaat aaattcaatg
    24241 aaagaattaa tgggattttt aaataatata tctgctaatc ctgaggatat aagtaatact
    24301 attatgtatt ttttaaataa aataaatgac tttatgaaat catttcaaga aatagccaat
    24361 tttgatgtag aacaattagt aaacaaattc agtggtgaag taatgcaaat tacaaaaaat
    24421 cttcttacta gtatacttgg attggctact tctattccat atattataat atttataatt
    24481 accttgttta ttgctaccta ctttatagca aaagaccttg ataagattga aaatagtttt
    24541 tataatatgt ttactgttga tgttagaaag aaggtaaaaa acgtaaagaa agaggcaggg
    24601 ctttcacttg ttggatatat aagggcctat acaatactta tggctataac attttttgca
    24661 atctggggaa gttttgcttt atttggattg aaatacggtc taatagtagg gtttgtagga
    24721 gctttaatgg atttaattcc attcctaggt ataataacaa tatatcttcc agtaattgtt
    24781 tattattttc ttataaaaaa ttattttata gctataagta tgactgtgat attttttgtt
    24841 ttgtctttga ttagagaaat acttgaacct aaactagtat cagtaaatgt tggactaaat
    24901 ccattagcta ccctagcagc gatttttatt ggaatccaag taaaaggaat aattggagta
    24961 attttttgtt tgggtttggt atgtatgcat gatatactta agaaggtgga tattttttaa
    25021 ttataataat tgatagattt ttttaaaaat aaaattttat tcacaggtaa ataaaaaaat
    25081 attaatctta ttaagaaaga gggtatatac gtgaaacaaa aagtagtgga aagattttta
    25141 aaatatgtat catttgatac aacatctaat tctcagtgtg agaactgtcc atcttcagaa
    25201 ggtcaaagag ttttagctaa gtatatagtt gaagagctaa aaacaatggg tgttgatgat
    25261 gtgagtttag atgaaaatag ttatataatg gctactttaa aaggaaatac tgatggtgtg
    25321 gatactatag gattcatatc acatttagat acaattgaag atgttagtgg aaaagatatt
    25381 aaaccaagaa taatagaaaa ttatgacgga aaagacatag ttttaaatga agcgttaaat
    25441 gttattacgt atgtaaagga ttctccagag ttggaagaat ttaaaggtga cgatttaata
    25501 gtcactgatg gaacaactct acttggctct gatgataaag ctggtatagc tgagatagtg
    25561 actgcaatag agtatctaat aaatcatcca gaaattaagc atggagatat aaaaatagga
    25621 tttacaccag atgaagaaat tggtagaggt gctgatttat ttgatgttga aaaatttgga
    25681 gcaaaatatg catatacttt agatggagga atagttggag aacttgaatg tgaaaatttt
    25741 aatgctgcaa atgcaactat tactatacat ggtagaaatg ttcatccagg ttcagctaaa
    25801 aataaaatgg taaatgctat acatatagct gctgaaatat cagaaatgtt cccagcagat
    25861 gaaagaccag aaacaacaga aggatatgaa ggattttggc atttaaattc tataggtgga
    25921 aatgtcgaga atgtaagtat ggcttatatt attagagacc attgtagaga aaaatttgaa
    25981 aatagaaaat ctattatgat agagaatata gaaaaaataa ataagaaata cgataataga
    26041 gtggaattgg atttaaaaga ttcttattac aatatgaaag aaaagattga gccagttatg
    26101 tttatagttg atatagctaa agaagctatg gaggaattag gtataaaacc acgtttagtt
    26161 cctgtaagag gtggtactga tggagcaaga ttatctttta atggattacc ttgtccaaat
    26221 atatttactg gtggattaaa cttccatggg aaaaatgagt gtataccagt tagttctatg
    26281 gagaaagcta ctaaactaat tgtaagaata gctgaaaaat atgctgaaag agtttagtta
    26341 aaaagtattt aaaaattaag catttaaagt tataatagct atatcattct ttataagtca
    26401 agctgattat aagcttgtgt tgtatttaaa atggtatagc tatttttgta attattttta
    26461 taaaagataa ttatttttag tttagggtat caataaaaag tatggatatc aataggtatt
    26521 aacatttaaa agggggaatt gtattgaaaa ttaaatctaa aaaaataata gcatttgcac
    26581 tttcacttgg gattatgttg ccaagttgtg tatatgcaga cactattagt aaaaatcaaa
    26641 atgcacaggt agatatgaac aattatcttc aaggtagaaa atctccaaag gaggaaaaac
    26701 ttattggtga agatagattt gatacagcga taaaaatatc tcaaagtggt tggaataatg
    26761 gttcagaaag ggtgtttcta gttaactcaa attcattgcc agatgcattg gcttcaactc
    26821 ctttggcaag taaattggat gcaccaatac tgcttacaaa taaaaatagt ataccatata
    26881 atgtaacaga ggaaataaaa aggcttaatc cagcagaagt tattttaatt ggaagtgaag
    26941 gagctatttc atcaagtgta aaagaatctc ttgaagatat ggaagtatta gtcagtagaa
    27001 tcggtggagc agatagagaa gaaacatctt tacttcttac tagacagcta gatgatactg
    27061 gtgatttagg tgtctcaaaa gtggcagtgg ttaatgggta taatggactg gcagatgcaa
    27121 cttcaatatc aagcccagct gcaagtgata atacagctat aatatataca ggtaaggata
    27181 gcataagaag tgaggcaaag aattttatta cacaaaactc aactaagaca tacatagtag
    27241 gtggggagta cagtataagt aaaaaacttg aaggacagtt agtaaactca gaaagactag
    27301 caggaacaga tagaaaagat actaatgcga aagttttaga aaagttttat ggaaaatcaa
    27361 gtaaggttaa taatatgtat tttgcaaaag atggaagtgg tagagaagct gatttagttg
    27421 atgggcttgc tgctggagta ttagcatcaa aaacaaagtc acctgtagta cttgcaagtg
    27481 gaagtttaag tagtgcacag aaaagtttta taaaaaaagt aaaggctgat aaatttgtac
    27541 aagttggtgg aggtaaaaat agtaaaccat atgcagaagc attagcctta cagtaatatt
    27601 gtgtaaatca aaattaatat ataataatag caagtaaacc aagttgaact tcaatatata
    27661 taataaaata agtaaactaa gttatatttc agtataatat ataatgaaat aagtaaacta
    27721 agctatgttt aaatataat
//
LOCUS       DAELSY010000036        27699 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.036, whole genome shotgun sequence.
ACCESSION   DAELSY010000036 DAELSY010000000
VERSION     DAELSY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27699
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.036"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            54..371
                     /gene="flgB"
                     /locus_tag="KPG09_001415"
     CDS             54..371
                     /gene="flgB"
                     /locus_tag="KPG09_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBG0966034.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYVTFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            379..783
                     /gene="flgC"
                     /locus_tag="KPG09_001416"
     CDS             379..783
                     /gene="flgC"
                     /locus_tag="KPG09_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBG0966035.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            793..1110
                     /gene="fliE"
                     /locus_tag="KPG09_001417"
     CDS             793..1110
                     /gene="fliE"
                     /locus_tag="KPG09_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBG0966036.1"
                     /translation="MPITQINNLMQGAKLDNLIGETKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1135..2685
                     /gene="fliF"
                     /locus_tag="KPG09_001418"
     CDS             1135..2685
                     /gene="fliF"
                     /locus_tag="KPG09_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBG0966037.1"
                     /translation="MIASFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDVQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAIPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDEDVDENNQPTNSLINVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSATNNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKAEAKQELEEIKKEGFKKNIIKAVIGVVAVVAIGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            2698..3768
                     /gene="fliG"
                     /locus_tag="KPG09_001419"
     CDS             2698..3768
                     /gene="fliG"
                     /locus_tag="KPG09_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBG0966038.1"
                     /translation="MSTEITNVNAFDLSDINESDKKEKIEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDVKKILEILRDESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFKDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQDPALAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEAAQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            3761..4492
                     /locus_tag="KPG09_001420"
     CDS             3761..4492
                     /locus_tag="KPG09_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBG0966039.1"
                     /translation="MNNKIIKSKKASYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            4496..5815
                     /gene="fliI"
                     /locus_tag="KPG09_001421"
     CDS             4496..5815
                     /gene="fliI"
                     /locus_tag="KPG09_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBG0966040.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGIAIG
                     EPPAQKGYTPSVFAILPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE"
     gene            5847..6293
                     /gene="fliJ"
                     /locus_tag="KPG09_001422"
     CDS             5847..6293
                     /gene="fliJ"
                     /locus_tag="KPG09_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBG0966041.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIESNLE
                     NLESNYSKYSISKNNDSVQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            6321..7526
                     /locus_tag="KPG09_001423"
     CDS             6321..7526
                     /locus_tag="KPG09_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBG0966042.1"
                     /translation="MKIQADSYNIIKDIGNRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KDRRETSKQNVSSDKRENSDNECHLDEDKSNHLEDENTYEKDCSNNNEALHQLLNLLK
                     DNYKKDKDINTILNNLKKLSISDELKQELKLNKNILNKIANQNLELNRASTNNPNDIE
                     NLDIMKLRELLENNASEDIEVISKTTENDTKSIDLYSFDSNRIDTLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKQNTNDKRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            7544..8152
                     /locus_tag="KPG09_001424"
     CDS             7544..8152
                     /locus_tag="KPG09_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBG0966043.1"
                     /translation="MYGIATALAGTQVNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMND
                     KLDILLALSNSTLINSALSMATSLIGKEVEVSGEEEEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDANSGEENDSSEQESPEEV"
     gene            8179..9162
                     /locus_tag="KPG09_001425"
     CDS             8179..9162
                     /locus_tag="KPG09_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBG0966044.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGVIYDGEYKADT
                     GEFIKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GNIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9175..9378
                     /locus_tag="KPG09_001426"
     CDS             9175..9378
                     /locus_tag="KPG09_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBG0966045.1"
                     /translation="MIRVTDLNGRRYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKVNVGYIRSEK"
     gene            9378..10196
                     /locus_tag="KPG09_001427"
     CDS             9378..10196
                     /locus_tag="KPG09_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBG0966046.1"
                     /translation="MKKTDSLTFIGLILSTVLVLFGAVKGSSSGLKSFFDFASILITV
                     FGSFGALMITFTIDDIKLIKDALQYSFKTMNISKLDLLNQFKTLSKKARREGLLSIES
                     DVMKIQDPFMKKGLELCIDGLEVNEIRSILELEIDSVEETNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLSDLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10186..10881
                     /locus_tag="KPG09_001428"
     CDS             10186..10881
                     /locus_tag="KPG09_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG0966047.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGISMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKADSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            10893..11336
                     /locus_tag="KPG09_001429"
     CDS             10893..11336
                     /locus_tag="KPG09_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBG0966048.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11351..11725
                     /locus_tag="KPG09_001430"
     CDS             11351..11725
                     /locus_tag="KPG09_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliO/MopB family protein"
                     /protein_id="HBG0966049.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMNKIKDLTKEEILRIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            11726..12391
                     /gene="fliP"
                     /locus_tag="KPG09_001431"
     CDS             11726..12391
                     /gene="fliP"
                     /locus_tag="KPG09_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBG0966050.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12404..12673
                     /gene="fliQ"
                     /locus_tag="KPG09_001432"
     CDS             12404..12673
                     /gene="fliQ"
                     /locus_tag="KPG09_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBG0966051.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            12685..14499
                     /locus_tag="KPG09_001433"
     CDS             12685..14499
                     /locus_tag="KPG09_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBG0966052.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTARMIFFSMDGHHVLIRGIRYSFELIPIGKSFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSSIEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPVSGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVLKR"
     gene            14508..16583
                     /gene="flhA"
                     /locus_tag="KPG09_001434"
     CDS             14508..16583
                     /gene="flhA"
                     /locus_tag="KPG09_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBG0966053.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVSKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAEIKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            16558..17574
                     /gene="flhF"
                     /locus_tag="KPG09_001435"
     CDS             16558..17574
                     /gene="flhF"
                     /locus_tag="KPG09_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBG0966054.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKLIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVVNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            17571..18449
                     /locus_tag="KPG09_001436"
     CDS             17571..18449
                     /locus_tag="KPG09_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBG0966055.1"
                     /translation="MIDQAQILRKMAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            18463..19164
                     /locus_tag="KPG09_001437"
     CDS             18463..19164
                     /locus_tag="KPG09_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBG0966056.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELNVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19180..19602
                     /locus_tag="KPG09_001438"
     CDS             19180..19602
                     /locus_tag="KPG09_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG0966057.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEIRSNNHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELSLIRSCYKI"
     gene            19587..19772
                     /locus_tag="KPG09_001439"
     CDS             19587..19772
                     /locus_tag="KPG09_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG0966058.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            19813..20586
                     /gene="flgG"
                     /locus_tag="KPG09_001440"
     CDS             19813..20586
                     /gene="flgG"
                     /locus_tag="KPG09_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG0966059.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNDYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            20607..21368
                     /gene="flgG"
                     /locus_tag="KPG09_001441"
     CDS             20607..21368
                     /gene="flgG"
                     /locus_tag="KPG09_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG0966060.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRIYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNIDGLGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21391..22272
                     /locus_tag="KPG09_001442"
     CDS             21391..22272
                     /locus_tag="KPG09_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG0966061.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMIVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVIKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTKKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22275..22649
                     /locus_tag="KPG09_001443"
     CDS             22275..22649
                     /locus_tag="KPG09_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG0966062.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELNTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            22681..24084
                     /locus_tag="KPG09_001444"
     CDS             22681..24084
                     /locus_tag="KPG09_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0966063.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNVKVDDYKNEFLHKSSSNHKIND
                     YNNVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEADVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            24200..26137
                     /gene="htpG"
                     /locus_tag="KPG09_001445"
     CDS             24200..26137
                     /gene="htpG"
                     /locus_tag="KPG09_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBG0966064.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITIQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            26525..27655
                     /locus_tag="KPG09_001446"
     CDS             26525..27655
                     /locus_tag="KPG09_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG0966065.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDIDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
BASE COUNT        11450 a         2927 c         5177 g         8145 t
ORIGIN      
        1 cattttttat taataagcaa aataaataaa atatattttg aggaggagtg agtgtgaata
       61 catatgacat tataaaaatg ggattagatg caactaattt aagaagtcag acaatagcaa
      121 ataatatagc caatataaat actgaaggat ataagagaca ttatgtaact tttgaagaaa
      181 cattaaatga tgctatgcca aaaatagaag aaaaaacaga caattctaaa ggattaagag
      241 tggatggcaa taatgtagat atagaagaag aaaaagtaaa tcaagcaatg gcaacattac
      301 aatataatgc tttgataagt tttgcaaatg gaaaaatagc aatgactaaa tcaataataa
      361 gtgggaggta attaaaaggt gagtgtgttc agtggaatga gaatatctgc tagtggatta
      421 acagcagaaa gcttgaaaat ggatatcata tcgtccaatg tagctaatat aaatgccaca
      481 agaggtccaa atggagaagc atataggaga aaagttgttt catttgaaga aaactatgat
      541 aataaattag gtatgctagg agtaaaagtt acatctgttg aagatgataa atcacctctt
      601 aaagaagtat atgacccatc tcatccagat gcaaagccaa atggatatgt agaatatcca
      661 aatgtaaata tattggtaga aatgacagat ttaataacag cttcacgagc atatgattct
      721 aacgtagata cattgaatgc tcagaagtct atgatatcaa aagctttgga aataggaaag
      781 tagggataaa ttatgccaat tacacaaatt aacaatttaa tgcaaggtgc aaagttagat
      841 aatttgattg gagaaacaaa accattaact cttaataata aagaagaaaa aaagaacttt
      901 gaagatgtaa taatagatac tatagataaa gttaattttc aacaagtgac agcagatgat
      961 atgaaagaaa aatttataaa aggcgaagat gtttctatgc atgaagttat gttaataggt
     1021 caagaagcac agctatctat gcaatattta atagaagtta gaaataagat tcatgatgca
     1081 tatcaagaaa tgaataaggt acaaatttag ccttttgaac aggagtaata atcaatgata
     1141 gccagtttta aaaataagat taaagagcaa gttgctaaat ctaggacaaa gataagttct
     1201 ctaaaaaagg gaactaaaat agcattaggt ataggggtta ttgctatatg tttagcgcta
     1261 gtatttacag caatatctgc gagcaaaaat aagtatgcag tcttattttc agaactagat
     1321 gttcaagatg ctgcagatat atcagcagaa ttagagtcta aaaagataga tactaaggta
     1381 aagggaagta cgatttatgt acctagaaca gaagttgata agttaaggct ggagctagca
     1441 ccaagtctta caggtggttc taaaggattt gcactaatgg atgatagttc atcaatagga
     1501 cttactgatg aagagtttca gattaagaag caaagaatga tacaaggtga aatagaaaaa
     1561 acaataaaga gttttccaca agtggaagat actagagtac atataacatt tggagagtca
     1621 tcagtatttg agaaggaggc tattccaggg agtgcagcag tttatttgat gttaaaacca
     1681 gggacgactt tggaaaaaaa ccaaataaaa tctgttatgg cacttatatc aggaagtgtt
     1741 acaaatatac cagagaaaaa tgttcaagtc attgataaca aaatgaattt attatctgaa
     1801 ggtattttag aggatgaaga tgttgatgaa aataatcaac caacaaatag tttgattaat
     1861 gttgataaaa ataaggcatc tgaacaagca ttgaataaac aattagaaaa gtctatttta
     1921 gaaatgctag aaccaatatt tggtaaagga aaagtaaaag ccacagtaaa tgctgattta
     1981 agttttgata cagctgaaaa aactgagata aaaattgacc ctgataaagt tgcaataaag
     2041 gagacaaggt cgaaaaattc atctaaatct gctacaaata atgttcaagg tgtagatgct
     2101 aacatgaata accaaggaaa taacaatggt actaatacag aagatagttt agatgagagt
     2161 tttgagtatg aaacaggtaa gattgaaact cacactatag ttgcgcctgg agaacttaat
     2221 agattgacag cttctgttgc aatagatgga aaaatagcta aaggtgtaca agctgatgta
     2281 gaggatattg ttaataatgc tataggtatg gatggagcga ggggagatac tcttagcgtt
     2341 gtttcaatgg tatttgaccc agaagggaag gcagaagcta aacaagagct agaagagatt
     2401 aaaaaagaag gatttaaaaa gaacataata aaagcagtta ttggagtagt ggcagtggta
     2461 gcaattggag ctattggata cttaatatat aagaaaagaa gtgaacgaga agatatggat
     2521 tatgaagatg aagattatat ttctaaatct gattctgttg ataggaagga taatcctcta
     2581 ttaggagatg acccagaact gacattagag gaagctgtta aaatgtatgc agaagaaaag
     2641 ccagaccaag taacagagat aataaaaact tggttaaatg agtaaggggg atagaaagtg
     2701 tccacggaaa ttacaaatgt taatgcgttt gacttaagtg atataaatga aagtgataag
     2761 aaagagaaga tagaaggtga gggtttaaaa aaagctgctg tattattaat gacattagga
     2821 ccaaatgtgt ctagtgatat aataaaaagc ttgcctgata aacaagttca aaaaataggt
     2881 ttagaaattg caaatatttc ttcaatagct tcaaaagaaa gaagagaaat cttaaaagag
     2941 tttgtagaga taaatagggc aaaagattat gttatagaag gtgggcttga atatgcaaga
     3001 gagttgttaa gcaatgcttt aggaaatgct aaagcaaaca aattacttga aggtatatca
     3061 ataaatacaa gtacaaagcc ttttaaacat attataaaac tagatgtaaa gaaaatatta
     3121 gaaatactta gggatgaaag tgcacaaact atagctgttt tacttgcaca tatggagcca
     3181 gaaaatggag cagaagtttt atcatactta gattttgatt tacaaaatga agtagctatg
     3241 agaataagct caatgggttc aatatctcca gaagtaataa aggtaattga aagttcatta
     3301 gaagataaat tacactcaat aagtggtgat gattttaagg attatggagg agtgtctacc
     3361 ttagttcaaa tattgattaa ttctgataga aaaacagaaa agaatatttt gggaactatt
     3421 gaagaacaag acccggcttt agcagctcaa attaaatctc atatatttgt ctttgaagat
     3481 attgtcaagt tagatgatat aagtatacaa aaagtattaa aagaagtgac tatgaaagat
     3541 ttagtatatg ccttaaaggg tgtggatgat gagatttcag atgctatata tagaaatcaa
     3601 tcttctagag cagcagatgc acttaaagaa gaaatggaaa tggttggtgt cataaaagtt
     3661 acacaggttg aggctgctca acaaaagata gcaaatatag taagaaaact tgaaagagaa
     3721 ggtgccataa ctattgtaag gaattttggt ggtgactttg ttgaataata agattattaa
     3781 aagtaaaaaa gcatcatata aaggagtgct agatttaacc ataatagata aagttagagt
     3841 gggagaatca aatgtaaata aagaaaatga ttgtagattt agagatgagg taataaaaga
     3901 agctatagaa tctgctgaga taaaaaagaa tgagatatta gaagaagcaa aaatgaatgc
     3961 acaaaatata gaaaaacaag catatgaaaa agggtatagt caaggtcaaa aaaatggata
     4021 tgaagatgga tataaagagt catatgatga atatacccaa aaagctaaag atgaagcaga
     4081 ttttataaaa aatcaggcag acttaatact attacaagct aatgagaaga tgactgagta
     4141 cataaaagaa aaaaataaag agattgtaag acttgctatt aatatggcta gttctgttct
     4201 aaaaaagcat ttcgaagatg aagaatctat gagtgatttg ttaaagcaaa ttattcaaga
     4261 ttatgaagga agtagttcat ttatagtaag atgtaattct ttctataaag ataatataga
     4321 gaaagagttt gaattattca aagaggaaac atcactaaaa tcaagtgtat ttgttattgc
     4381 agatgatagt atagataaag gcaatgctat catccaaaag gaaaatggaa gaataatagt
     4441 tggtatcgat tgtgctatgg aaaaactaaa agaagaattg cttggtgaat aaagtatgta
     4501 taaaactgat atatctatag attttgacaa aataaataag aaaatagaaa atttagaact
     4561 cactatatca gaaggtagag taaagaagat aataggactt actgtagaag ttgaaggaat
     4621 aaaagctttt gttggggagt tatgtgttat atacaatcaa gtcaataaac cagtgaattg
     4681 tgaagtagtg ggttttaaag ataaagaagt catattaatg gcgttgggag agctaacatt
     4741 gatagctcct ggttgtaaag ttatttctaa gggaatacct ttaagtgtta tgtgtagcga
     4801 taatttacta ggtaaagtac ttgatggtct tggaaatcct atagacaact cagatgctgt
     4861 tttaggagat agatacaact tgaacaatga acctcctgac ccaatgaaga gaaaaaagat
     4921 acgaaatata atggaaacag gtgtaagagc aatagatgca tttactactt gtggtgaagg
     4981 acagagaata gggatatttg caggaagtgg agtaggtaag agtacgactc taggtatgat
     5041 tgctaggaat gcaaaagcag atgtaaatgt aattgccctt attggagaaa gaggaagaga
     5101 ggtactagat tttattgata aagatttagg tgaagaggga atgaaaaaat ctgttgttgt
     5161 atgtgcaaca tcagataaag ctcctttggt taggctaaaa ggtgctctta ctgctactgc
     5221 aatagcagag tattttagag accagggaaa aaaagtaata cttatgatgg attcagtaac
     5281 tagatttgct atggcacaaa gagaagttgg tatagcaata ggagaaccac cagctcaaaa
     5341 aggatataca ccatctgtat ttgctatttt accaaaactt atggagagaa cagggacatc
     5401 agataaaggt tctataactg cattttatac agtattagtt gatggagatg actttaatga
     5461 gccaatagca gatacaacaa gagggatact tgatggtcat atagttttat ctagagactt
     5521 ggcaaataag aatcattatc cttctataga tgtattgaac agtttaagca gacttatgaa
     5581 tgagattgct tcaaaagaag acataaaaat agcatctttt gcgagagata tgcttgctga
     5641 gtatagagaa gcagaagatt tgataaatat aggtgcatat gcttcaggta caaacaaaaa
     5701 aatagatgaa gcaatctact atcatgagca tattattaat tttttaaaac aaggaataaa
     5761 tgaaaagagt tcttttaatg aaactataag tagtttaaga agaatctttg aataaacaat
     5821 ttaaaaaaat atttgaatag gttgtgatgg aattggataa aaattttaag tttagattac
     5881 agaaagtttt agacttaaaa ataaaagatg aagaagagat aaaaatggaa tttgctaaga
     5941 ttcaacaaaa aaaaatagat atagagagca atttagaaaa cttagaaagt aattacagca
     6001 aatattctat aagtaaaaat aatgatagtg ttcaaaatca aaaaataact attaactatt
     6061 tgttagcttt aaacaactct ataatggatt tatcagaaga gctggataag tcgacaaatg
     6121 aacttgaaaa agctagaaag cagcttataa gcaaacagat agagagaaag tcattagaaa
     6181 aattaaaaga aaaaaaatat ggacagtatt ataaagaaga acagttaaaa gaacaaagca
     6241 ctaatgatga gtttgcaagc atgagttatt tgagaaatag acaagttcta taaaatagta
     6301 ggaagtgagg atattattgt atgaagatac aagcagattc ttataatatt attaaagata
     6361 ttgggaatag aaaaagctca tctaagaata aaaatataaa aaataataaa tttgaagata
     6421 atctatataa ggcaacagac cataaaaagg ttaaagatag aagagaaaca agtaagcaaa
     6481 atgttagttc agataaacgg gaaaatagtg ataatgagtg tcatttagat gaagataaaa
     6541 gtaatcactt agaagatgaa aatacatatg aaaaagattg tagtaataat aatgaagctt
     6601 tacatcaatt actgaatctt ttaaaagata attacaaaaa agataaagat ataaacacta
     6661 tattgaataa tctaaaaaaa ctaagtatat cagatgaatt aaaacaagaa cttaagctta
     6721 ataaaaatat attaaataag attgcaaatc aaaatttaga gttaaataga gctagtacaa
     6781 ataaccctaa tgatatagaa aatttagaca taatgaagtt aagagaactt ttagaaaata
     6841 atgctagtga agatattgaa gtaattagta aaactacaga aaatgatact aaaagcatag
     6901 acttgtacag ttttgacagc aatagaatag atactcttaa ctcaaataaa cagaaagatg
     6961 atagttctga tattttggaa aaactagcag gggtaaatgg aaataagagt ggaaattttg
     7021 gacaagtaat aaataaaagc gttgatatga ataaaattaa aaatgataat gttaatttaa
     7081 aacagataga atcaaatgta atggatgatt ctataaaagc tattaagcat atgaaaacaa
     7141 atgatataca agaattgaca attaaattaa gaccaaaaga attaggagat atgaacatac
     7201 aattgttaaa agatggtgaa agtatgagag ctgttgtgac tgttttcaac aaagatgtct
     7261 ttgactctat aaataaaaac atagcagatt taaagcaaca tcttgaactt acaaatgtaa
     7321 atataaaaga tgtatcagtg caaatgcata gcgataatac aaatacatct gatacctttg
     7381 ataaagcatt tgaacaacag aatagacaaa ataaccaaga aaacatgagt aagcaaaata
     7441 caaatgataa gcgtaacaat gtagtcatag aagatgagat taaagaaaat attattgatg
     7501 atgatagagt agatttacta gcttagtaag ggggaagtat tgcatgtacg gaatagcaac
     7561 ggctttggca ggaactcagg taaactatag gtcaaataat ttaagccaac ttgaaagaaa
     7621 tattaatgat attgccaata aacaagttag aagtaaagga aatcagacag taacagacaa
     7681 tgggactcca attataggac aaggcgatga tgctgataaa gatttgttta tgaagattct
     7741 tgtagcacag atgggtaatc aagacccaat gaatccacaa gacccaacag aatatgtatc
     7801 acaattagca cagtttgcaa ctttagaaaa gatgtcagct atgaatgata agctagatat
     7861 acttttagcc ttaagcaatt ccactctaat aaattctgct ttgagtatgg caacatcatt
     7921 aattggtaaa gaagtagaag tatctgggga agaggaagaa gatgaaaaca ttgtaggagt
     7981 agtaaaatcc acatatataa aagatgggga agtacactta gaattagaag tagatggaag
     8041 tgacaaaact gtaggtgtta aatatgaaaa attacttaaa ataggagagg tagatgcaaa
     8101 ttcaggagaa gaaaatgact cttcagaaca agaatctcca gaagaagtat aatattaaat
     8161 gagaaaagag gtaaatttat gataaaagca atgtattcag gtgtaagtgg aatgaaagca
     8221 aatcaaacaa aactagatgt aataggtaac aatgtagcca acgctggaac tacttctttt
     8281 aagaaaagtt cagctagaat aacagattca ttttatcaaa ctgtactata tgcaagtgct
     8341 ccaacagcgg cattaggggg aacaaactta ggacaagtag gtgtaggtag taagatatca
     8401 agtataaata aagatatgac acaaggaaat gtacaaccaa ctaataggcc atctgattta
     8461 atgatagatg gagatggata tcttccagta gtaagacaag gaacagttat gtatacgaga
     8521 gatggttctt ttaatttaga tacaggattt aaagcagctg atggtgcttt gaagaatatt
     8581 aaaggtggaa gacttgtaaa tggtgatgga tatatgttac aaggtgtgat ttatgatggg
     8641 gaatataaag ctgatactgg agaattcatt aaaaagggtg atgctaaagg aaaagagcct
     8701 attgtaatac cacttcaaag tataagtcct aaggatggaa cagttcaaaa tgtactagag
     8761 tataatatat caaaggatgg aacagtagaa tttttattga gtgatggtca aagaactcaa
     8821 tttgtctatg atatacctga tggtgctaag gtaggagata cacctgctgg aatagataaa
     8881 ttaaaaggta caatacaaaa agtgcaaaca tatgcattcc aaaatccagc aggactagat
     8941 gcagaaggag gaaacttatt caagccatca gcaaactcag gagatccaca aatagcagga
     9001 gctacaggaa acataataca aggggcaata gaagcttcca atgtagatat agcagaagaa
     9061 tttacagaaa tgataatagc aagtaggtct ttccaagcaa acagtaaaat aatatcgaca
     9121 tcagacgaaa tacttcaaga aataataaat cttaagagat agtaggtaca tattatgata
     9181 agagtaacgg atttaaacgg aagaagatac atattaaatt cagaccttat agtgagaatt
     9241 gactctgtac cagaaagtag gattctcctt acaacaggag aaaaaaatct tgtaaaagag
     9301 tcagtagatg agatagtaga taaagtaata aagtataaaa gaaaagtaaa tgtaggttat
     9361 attaggagtg aaaaataatg aaaaaaacag attcactaac attcattgga ctaatattat
     9421 ctacagtatt agttttattt ggggcagtaa aaggaagtag ttcaggattg aaaagttttt
     9481 tcgattttgc atctatatta atcacagtat ttgggtcatt tggagctttg atgattacat
     9541 ttactattga tgatataaaa cttataaaag atgcgctaca atactcattc aagacaatga
     9601 atatatcgaa attagactta ttaaatcaat ttaaaacact ttctaaaaaa gcaagaagag
     9661 aaggactttt atctatagaa agtgatgtaa tgaaaataca agacccattt atgaaaaaag
     9721 ggttagagtt atgtattgat ggccttgaag taaatgaaat aaggtctata ctagaactcg
     9781 aaatagattc tgttgaagaa accaataaca gatattctaa actatttaaa acttggggga
     9841 ctttcgcacc tgcatttggt atgttgggaa ctctaatagg tttaatacaa atgttatctg
     9901 accttacatc tccagattta atagcatcgg gtatgggaaa agcattaatt acgacatttt
     9961 atggttcttt acttgctaat atcgctttga acccaattgc atataatata gatgagaaga
    10021 ctgaaaaaga gatttatgta aaggaaatga tgctggaagg tataataagc atacaatctg
    10081 gggagagcag catagtagtt gaggaacgtt tagctacata cctttcaaac aatgagaagc
    10141 tagaaattat gaaatccaat aaaaatacag aaagagcaat gagtaatggc gcgtaaaaaa
    10201 aataagaaaa aggatgagat aaatccagat gcatggttgg ctacttatgc agatactata
    10261 acactgattc ttacattttt tgtgttattg tatgctacat cagttgtaga tatggaaaaa
    10321 tttaaaaaca tagcagatgc gttaaaaggt cagttttctg ggataagtat gttcagtaca
    10381 ggttcaagta caagtgagaa gtctccaata gagagtgttt tagtggataa aaatgataag
    10441 tcaaatttag aacaggattt aaaagataaa gttaataatt ctaacttaca aaatagtgta
    10501 acagtcaaag atgatagtag agggatttta ctagaactag atgattcaat tttatttgac
    10561 tctgggattt ctgaattaaa agcagatagt aaagccactc ttaataaagt atatgacatg
    10621 ataaagatga tgaaaaataa gatagttata gaaggccata ctgataatgt acctgtaaaa
    10681 tcaagtacac atgaatccaa ttgggattta tcttctagta gagcagtaag tgttgtaaga
    10741 tactttgtag aagaaaaggg catggaccct agactatttt cggcaacagg atgtggagag
    10801 tatcaacctt tggtttcaaa tgaaacagca aagggaaggg ctgagaatag aagagttaat
    10861 atacttataa ctattgacta ggaggaagag gtttgttcaa aaataaaaaa attattataa
    10921 ttattttgat actgattatt ttctcagcag gagcaggttt ttttatatta ggagaaggtg
    10981 gaaataaaca aattgacaag attgcaggtt tgtttattcc aacagggaaa ggcaaagatg
    11041 aagtaaagac cgaaaatctt gacttagaag agatggtatt aaagctagct gatgaaggtg
    11101 taagatattt aaaagtatca ataactgtat cttatgattc ttcatataaa gatatagaaa
    11161 aaaatacgcc tttaattagg gataatatta taaattgttt tatggcaaaa agggcagatg
    11221 attttactgc tgataattta aagaacataa aagaagaaat taaaaataca ttaaatatac
    11281 aactaggtga aaatgtaatt caaaatatat atttcacaaa tatagtagtg caataaaagg
    11341 atggtaagac atgatacttt atttactaaa aatattaata tgtatcccaa taattttaat
    11401 attgatagtc ttatctttaa aaattagcca aggaacttta ttaaaaaaat ataatgataa
    11461 atatataaaa atactagata ttgtaaatat aagtaaaaac aatagcataa taattttaaa
    11521 aataggtgaa gaaggatgca ttatggcatc ttcttcctct agcatgaata aaataaaaga
    11581 tttgaccaaa gaagaaatat taagaataga aaacgacatt aaagaaagaa attttaaaat
    11641 tcaagatttt aaaatggaaa gaattaaaaa gaaatatgat ttcagtagat atattggaaa
    11701 atttacaaaa aaggaagata agtaaatgga actattatca aaattacctt tggctgatgt
    11761 accatataca agttcaatgc aattgttttt atttctaact gcattaatgt ttttaccatt
    11821 tgtaatgttt atgatgacaa gctttgtcag aatagttata agtttatcat ttttgaaatc
    11881 agcattagga gcacagcaag caattccaag ccaaattttg gtagggcttg caatagtatt
    11941 gacaatattt ataatgagac cagtattaaa tgagataaac gaaaaggcat tacaaccata
    12001 tatgaaagaa gaagttacta tggaagaagc tatgaaggaa gcagagggac ctattaaaga
    12061 atttttatta aagcaaacca gacagacaga tttagattta tttgtagaac aggctggttt
    12121 gaaagaaaag aaattaactc gtgaaaatat accactatct gttgtagttc cagcatttgc
    12181 tataagtgaa ctaaaaactg cttttcaaat aggtttttta atatatatac catttttgat
    12241 aatagacctt gttgtggcaa gtgttttgat gtctatgggt atgtttatgt tgcctccagt
    12301 catgatatca ttaccgttta agttattatt atttgtaatg gtggatggat ggaacttgat
    12361 agtaaaaaca ttgatattgg gatttggata ggagaaaaat agtatgagtg aatttatgat
    12421 aatgagtgtt cttaaaaatg gcatgtttac aggagctatg attgcagctc cagtactcat
    12481 tgtttctctt gtagttgggc ttttaataag tgtgtttcaa gctacaactc aaatccaaga
    12541 gcaaactttg acttttgtac caaaactaat agcaattctt ttagtagtag ctattggtgg
    12601 aagctggatg ttacatactc ttattagctt tacttcggag atattcaata tgatgtctct
    12661 tataactaaa taagtaggat atatatgatt tttgtattta ttagaatagt ggcattcttt
    12721 ggaacactaa ggatactatt tccagctggt acacctgctt tatttaagtc gctctttgca
    12781 atcattatat caatattgat ttcaagtact atgcaaattg aatatgcaac aaatatagac
    12841 aacattgtat tgtttacctt atatggtata aatgagacca taactggcat tatgcttgga
    12901 tatataacca atctatgttt ttatagtatc agaatggcag gttctatgat ggatcaacag
    12961 atgggattat caatgattaa tatgtttgac ccaaattcaa tgactcagac aactttaatt
    13021 gataatctta tgaactggac agcacgtatg atatttttta gcatggatgg tcatcatgtt
    13081 ttaattagag gcataaggta tagttttgag ttaattccaa tagggaaatc attcgtagat
    13141 aataatatag attatataat aaacatattt gtgcagtgct ttttaactgg attcaaaata
    13201 gcaataccta ttgtactttg tctgcttatg gcagatttta tattagggct tatatcaaga
    13261 agtgtacctc aactaaatgt aatgataatt ggtatgccat taaaaatact ggttggtatt
    13321 gcgttattta tcatatcaat acctttaatt gtcaatcaaa taagtcattt attaagtcaa
    13381 ataccaaaga tgtatgaggg aactttcgca ctagctccta tgttttttat gggttcaaca
    13441 gataaaacag aagaggctac tccaaagaaa aagggagaac aaagaaaaaa aggtaatata
    13501 gcaaagagta aagaattacc cgttgcaatg actctacttg cattcacttt attagtacct
    13561 acattatttt catatgtagt ggatacactt aaatcttctc ttaattattt tttgagtcta
    13621 gatttttata tgaatataaa ttattcaagt atagagaaat tagttatagc aggattaatg
    13681 gattttttta agatattttt accaatagct attccattct tagttttagg tatagtcgca
    13741 aacctatttc aagtgggaat actgtttact ggtgaaactt taaaacccaa tttatcaaaa
    13801 ttaaatccag ttagtggatt taaaaacatg ttttctatga gaagtttgtc aaccttaata
    13861 aaggatacag cgattatatc tattttggct tacataggat atacattctt ccaagataat
    13921 tatttagata tattaaagct aggtaacata tacctgccaa ctttaatgta cacagttaaa
    13981 gatttagtat attcaatatt aagtaagatt tgtgtagcta tgatagttat agcagttgca
    14041 gattatgttt atcaaagata cagtcataaa aagcaactta gaatgacaaa acaagaagtt
    14101 aaagatgaat ataaaaactc agaaggagac ccagaaataa aagctaagat aaaacaaaaa
    14161 caaagacaaa tttcgtctca aagaaccatg caagcagtac caagtgctac tgtaatagta
    14221 accaatccca cccacttatc tatagcagtg agatatgaaa aagggaaaga ccaagctcca
    14281 gtagtggtgg caaaaggtgc agattattta gcttttaaaa taagggaaat tgcaaaggga
    14341 aatgacatac ctataataga aaataaacct attgctaggt tgttatataa gcaagtagaa
    14401 atagaccaag aaattccaga ggacatgtat caagcttttg cagagatatt agtagcagta
    14461 tataaaatca aaaatagata caaagtcctc aaaaggtagg ataagtaatg aataagttta
    14521 atttaaagga aaatatggac gtaatagtgg catttggtgt tgtagggatt gttcttatga
    14581 tgatagtgcc gttacctaca tttatattag atattttact ggcaattaac ataagtttat
    14641 ctgtgctaat tttactaatg acattattta caacaaatgt acttgattta tctatatttc
    14701 caacagtgtt acttgtgaca acactattta gattgggatt aaatctttca tcaactagat
    14761 tgattttaac acagggatat gcaggtgaag ttatagaggc atttggaagt tttgttgtag
    14821 gtggaaatta cgtagtagga ataattatat tcttaattat attaattgtt caatttgttg
    14881 ttataaccaa tggttctgga agagtatctg aagtaacagc tagatttacg ttagatgcca
    14941 tgccaggtaa gcagatgagt atagatgcag atttaaatgc aggagttata gatgacaaaa
    15001 ctgctagaaa aagaagaaaa gagcttcaac aagaagcaga tttttatggc tctatggatg
    15061 gagcaaataa gtttgtaaag ggagatgcca tagcaggtct tatagtaaca gcaataaaca
    15121 taataggtgg aattgtaata ggagctgtaa tgctgaatat gactcttatg ggtgcagcac
    15181 agacatatgt aagacttact ataggggatg gtcttgtaag ccagatacct gctctattaa
    15241 tatctacatc agcgggtata atcgtaacaa gagcttctac tgatgaaaac tttggtggat
    15301 tagtaagtaa acagttaacg gcaataccaa aagcagtaat gatgactggt tgtgttctaa
    15361 tcgtacttgg tatgatgcct ggattaccta aaccagcatt tttttcacta ggtataggtg
    15421 ctatagcagc aggttacttt ttaggaagag aaaagaaaga tactttagaa gacttggagt
    15481 ttgaaaaacc tgatatggta aatccagata aaaatgaagt agaagatgta acaaacttaa
    15541 taaatgtaga atcaatagaa gtagaaattg gatatggttt aatatcttta gctgatgaat
    15601 cagcaggagg agatttactt cagagaatag catcaataag aagacaatgt gcaatagaca
    15661 tgggtatagt cgtgcaaccc ataagaataa gagataattt acagttaaat ccaaaccaat
    15721 acaatataaa aataaaggga aatgtagttg gtgggtatga gataatgcct aatatggttt
    15781 tatgtatgga ccctatgaat caaggattta gtatatttgg aataaagaca attgagccta
    15841 cttttggaat agaggcactt tggatagaaa gtgacaaggt agaagatgca gagataaagg
    15901 gttatacagt tgtagattca tccacaatac ttataactca cttactagat ataataaaat
    15961 caaatgcaca cgaattactg ggaagacaag aagttaagac tattatagat gcagcaagag
    16021 aaaattatag tgctgtagta gatgaattga taccagattt aatgacactt ggtgaaattc
    16081 aaaaagtact taaaaatctg ttaagagaaa aagtaaatat aaaggatagg gttacaatac
    16141 ttgaaacgct tgcagaccag tctagaaata caaaagatat agagcttctt actgaatatg
    16201 taagaatagc cttggctaga agtatttgta ataatttagt tgatgatgat aagacaattg
    16261 tagtggctac attgtctcca gaaactgaag agttggtatc taataattta caaaggtctg
    16321 taaatggaac ttatccagca gttgacccag aaaatacaaa taaaatattt gagagtatac
    16381 aagaagtaat gaacaatgta tatttcaatc ataatatacc agtaatagta gtatcaccaa
    16441 agataagagc tccatttaga aaattagttg aaattgtata tcctaatttg actgtattat
    16501 cattaaatga aatacctaac gatattaaaa tcaaagcaga aggtgtggtg agcatatatg
    16561 atgacgaaaa agtatacagc taacacaatt caggatgcta tgaatctagc caaaatggaa
    16621 ttgggtgaca atattacatt aatagataaa aaagaagtaa gaaagtctgg aatacaaggt
    16681 atcttttcaa agaaagatat tgaactaact attggatggg aaaagagaga aaacgtagaa
    16741 caaaaagatt taaaacgaga gatagaacaa ttaaagttaa ttattaacaa tatggggttt
    16801 gataataaga atgataatga tatagataaa atatgtaaaa atctactaag tttagaatta
    16861 aatgaagaaa ttgtagaatc tataagaatt gatttgcaag aaatgaagtt caatggaatt
    16921 gatacaagca aaaatttagt agaaatttta aagaaaaaga ttaaaataga aaatcaagca
    16981 ataaatggta aaattgctct agtcggacca ccaggagtag gtaaaacgac tacaatagca
    17041 aagttagccg caaaattagt ttttgaagaa aataaaaaag taggagtaat aactatagat
    17101 acatacagga ttggtgctgt agaacagttg aagatatata ctgatataat gaatattcct
    17161 tttaaagggg taataagtcc cgatgaaatg gaattggcac ttgatgagat gaaagattgt
    17221 gatgttgtat taatagatac aacaggtaga ggctataaaa actctatgca aatactagaa
    17281 atcaaaaatc ttatagataa agctgaaact gataatatac atttagttgt gaattgtaca
    17341 acaagagaaa gtgatacaaa agcaattata gattcatata gaaatgtgaa ttttaaaagc
    17401 ttaataatta caaagctaga tgaaacaata acatatggct caatatttaa cataatgaat
    17461 tatgcacaaa aaccaatatc atatattaca acaggacaaa atgttcctga tgatattata
    17521 aaacctaatg aagataagat tataaggtta ctattaggag ttgaaagtat atgatagacc
    17581 aagctcaaat tttgagaaaa atggcaatgg aaaaaagagg acttgatgag tttatagtag
    17641 agaatgagaa tgtacctaag ataattacaa tagcttctgg taaaggcggt gttggaaaga
    17701 gtaatctcgc tacaaatttg tctatatgct tgaccaaact taataaaaaa gttcttatat
    17761 tagatgcaga cataggaatg tctaatatag atataataat gggggtaaat gtaaaaggga
    17821 ctataattga tgtaattaat ggcgaaaaaa atatagaaga tataatttca cagactaagt
    17881 atggtgtaaa tataatatct ggaggttctg cccttaatca tatagaagac tttacagaag
    17941 ctcaaagaaa taagtttata catagcatag aacagattca cgatgtggat tttataataa
    18001 ttgatacagg agctggtatg agcaaaagcc tattgtcatt tatatactgt agtactgaat
    18061 ttttcttaat aacaactcca gaaccgacat cattgactga cgcatatagt ttattaaaag
    18121 ctataagtaa ttttggcata aagagaagtg caaatataat aatcaatagg gtaattgatt
    18181 tagaagaagc aaaatctaca tacaaaagaa taaaaatggt tgtggacaaa tttctaaacc
    18241 ttaatctaga attgtatgga tatataatag atgacaaaaa agtaagttta tgtgtaaaaa
    18301 aacagatacc attcatttta gaatatccta caagtattgc atcaaaatgt attatagcca
    18361 tagcaaagag aatggtaagt cagtcaagag aagatattgt tgataataaa agttttctaa
    18421 gaaagatgtt tagtatattt aaaagctaag gaggcgtagt taatgaatag agaagaatta
    18481 ataaaagaga atatgcctct tgtaaagagt atagcaagaa aattttttat accagggaaa
    18541 ggttttgaat atcaagactt agtaaatagt ggagtcattg gattgattga tgcaataaat
    18601 aaatttgatg ctgaaaaggg ggcaaagttt tctagttact cttatataaa aataaaatct
    18661 gctatattag atgaaattag aaatcaaagt ccaatttcta aacataatct aactaaagta
    18721 aataagtata atagagtggt agaaaagttg caatctaaat tattaagaga gccaacttca
    18781 tatgaaattg caaaagaatt aaatgtttca gaaaaagaac ttcatgatat cgaaagtaat
    18841 atagacatgt taaatattgt atcattaaac tatgtaatct ttgaagatac aaatgaaact
    18901 gtacaagatg taataagtga tagagaagag gaagctccag aaaatataat agaagaagaa
    18961 gaaaagttag agattctatc taaagcaatt agtaatttaa atgaaagaga aaaattaata
    19021 ctttcactgt actattatga agatttaaat ctaaaggaga tagggaaagt gctaggtgtt
    19081 tcagaatcaa gagtttcaca actccacaga aaatcaataa gaaacttaag aaacaagata
    19141 aaagaactta aatattctat atagatggtg attgataaga tgatacaaat tatattaata
    19201 gtagtgtcta ttttaataat agtaactata gttataaatt tacataagga atcaaaaaat
    19261 aaagataaca gatactttga taaaatattt gagcaagaat atgaagcaaa tgatagatta
    19321 ataattgcag aaaaaagaag attcttttta gacaaagtac ttgctgaaat aagaagtaat
    19381 aatcatataa aagtttatga aaattataac ctaataaagg ctaatgaaga aagaaaggaa
    19441 aacatattta tagaatcaaa aaaggaagta agacaagaaa atgagttatc tcttaaagtg
    19501 aaacatctta taagttcagg ttatgatgat gtggaaatat gtaagatgct agatattgga
    19561 aagggggaac tgtcattaat aaggagttgt tacaaaatat aaggatatta ttatgtgata
    19621 aaaaagtatt cttaggaata ggtattggta tattaatatc aactttatgc ataatgcttt
    19681 ttagtaataa tgatatgagt aaggcagaga tagaaaaaaa ggccagagcg ttaggcatga
    19741 aatatacaag tgagatgaaa gttttagaat agatgatact gtttagacat ttacaaaaaa
    19801 ggaggtcata atgtgataag aggattatat acagcagttt ctgctatgat aactaatcaa
    19861 caaaagcaaa atgttgttgt caataaccta gctaatatgg atacaaatgg atataaaagt
    19921 aaacaacttt tgactaaaag ttttgatgaa ttaaagttgg agggttacaa taactacgca
    19981 aatggaacta aacaaaagca gattataggc gatataagtc caggtatttc tatggatgaa
    20041 actatcacaa attttaatca aggacctata aaaactactg ataataaaat ggatgtagct
    20101 attcaaggta agggattttt tctagttagt gatgcagctg gaaatcaatt ttacactaga
    20161 aatgggaact tcagaacaga caatcaaggt gaccttataa caagtgaagg gtattatgta
    20221 cttgggacga atactgctac tggtgctgta ggtcatataa atgtaggaaa tcaaaaattt
    20281 gaagtggcaa aagataatac aatatcacta gatggaaatg ctatgtataa attcaatatt
    20341 gtagatttta atgactacaa taacttaaaa aaagaaggag ataacttgta ttctggagga
    20401 ggagctatga aagtaaataa tgcacttaca atacaaagtg cgatagagtc ttctaatgta
    20461 gatatggtat ctgaaattaa taatatgatg accatatcaa gagaatatga ggcaaatcaa
    20521 aagataattc aggctatgga ttctaaatta gcaaaaatag caagtgagat tggttcagta
    20581 agataggtga taggaggtga atttttatgt acacaatgtt ttatactagt aaaacagctt
    20641 tatcagcaaa tcaaagtaaa ctagatataa tatctaacaa tatagcaaat tctgattcaa
    20701 caggatataa aaaaataaat atggaatatt ctgatttagt ggatgaagta ctaaatagac
    20761 catcatatcc tacaaatggc aatgatatat cgactggaac aggagctaag gcaacagctc
    20821 ccatgagaat ttatgaacaa ggtgcattgg cccctacaga tagtaagagt aatttggcga
    20881 tagatggaga aggattcttt agagtgataa gaaaagatgg tacatatgca tatacaagaa
    20941 atggtggatt taatatagat ggtttaggta agattgtaga tgataatgga aatatattag
    21001 atgttcaatt tgatgcagga tataactata ataatacgaa tatagatagc acaaacttaa
    21061 cagtttcaag aaatggcgag ttattcgtag ataataaaaa aataggaaaa atcaatttat
    21121 atcaacctat aggaactcaa aattttatat ctgaaggaga tagtttattc gtcgcaactg
    21181 atgcagcaca gattaaacaa gttgagaaag tagatatagt tcaaggctat agagagaggt
    21241 caaatgtttc tttacaagaa gaatttgttg acttaatagc cactcaaaga gcatttcaaa
    21301 tgaactcaaa gggaataaag acagcagatg aaatgtggca aatagctaat aatttaagag
    21361 ctaaataatt atgtaaggtg aggaatattc atgaatgata tgaaagagta cgattttggc
    21421 aaacctcaga gttattcaag agaaaattta acttgtttaa attttttatt agctgaattt
    21481 tgtaaaaaat ataaaaacta tataatttat gaacttaagt gtaattctag tatgattgtt
    21541 gatagtatag accaagtaaa ttatcagact tttttagata gagtaacttc ttcatgtgta
    21601 gtagtacaaa atgcaatgga acctgttatg aaaaactttt cttttagaat agatagaagt
    21661 gtagctgata tgtggattga tataacttct ggaggaactg gagttataaa ggataatgat
    21721 agagagctta cagaacttga taaaaaagtg cttttacatc ttatggatga tttagtaaga
    21781 aatatgcact tatttgaagg ttttgaaaat atacaagtat tggatacaca tacacatatt
    21841 aatttaccac aattatgttc tccaactgca ccagtatgcg ttgtagattt aaaagtgcta
    21901 aatgataatg agtatattgg aagtgtttct ctttgttttc cgtataatgg tttagaggct
    21961 ctattagaaa gtatatctgt tatagaattt tttgataatg atgtaggaga tgattcagag
    22021 gaatttacca aaaaaatcta taatagtgta tctaatatag aattatcagt tattgcagag
    22081 ataggaaaag tatcaattaa tgttgaagac ttacttaaac tagaagttgg agatgtaata
    22141 gtaacaaata aaaaaataaa tgattatata gatatatttg tagaaaattc aaaatcatat
    22201 acagcaacac caggatttat atcaagcaaa aaaggtgtaa aaataaatga tgctgttgga
    22261 aaagaggtgt agttatggac aatagtaatc ttatttcaga tgaagaagca aagatgttat
    22321 taaatgataa agttgaaagt gaagttgatg gggatgatga gttagcctta gatgtctctg
    22381 aagttaatcg aaataatata caaaggatac ttgaccttaa gttagaatta tctgtagtta
    22441 tagggagaac taagatgact ctaagagata ttttaaattt acataaaggt tctcttattg
    22501 aactgaatac tttagcagac caagatgtgg aaatactaat agataataag gttttggctt
    22561 atggaaaagt agtggtggta gatttaaatt ttggtgtaaa gataacaagt atagtaagtg
    22621 aagaagacat ggtaaaatct cttgtttaga gataacatca attaatactt aaggcatgag
    22681 gtggtactta ttttaaactt aaatgatata aaaaaagata tactaaaact caataaaaaa
    22741 gactcatgtt ctctatataa tgaatctatt aataatataa ataataattg tatatcaact
    22801 ggattaaaat ttttaaaaca agcaagagat ttaaacccag atgatgttga tatattaaat
    22861 ctcacaggtc ttgtaaactt actaaaatgt aattttgatg atgctgtaga aagtttctat
    22921 aagagttcac tttgtgaaaa aactgctctg tgtgaaaagt atataaacat gcttacatct
    22981 gaggagtttt taattttctt tgaaaagtac aatcaactta taaggttaat aaataaagga
    23041 atgtataaag aagctataca agggtttaaa cttataactg accaatactg tgatttaata
    23101 gaaccttatg agttgcttgc cttactgtat gacaaggaag aagaatatat aaaatttgat
    23161 gaatgtttag aagtattgaa gattattgat aaggaaaatt atttattaaa tcaagaaaat
    23221 aacacattag atatgtataa tagtgctaat atacaaacta aaagtaatgt aaaagtagat
    23281 gattataaga atgagttttt acataaaagt agtagtaacc ataaaattaa tgattataat
    23341 aatgtaacta aagaaaaaag ttcaagcaaa aaccagaaaa gttcagattc taataagaaa
    23401 aaaagtatta aaaaaaataa gtacctatat atagccataa tattaggaat attattagta
    23461 ggacaaagta tatatagttc ttataagatg aataatttaa ctgataaaat atctaaaaat
    23521 aatgaaacta aaaatgaaga gtcaatagat tctaacttga ctaagaaaga aaataatata
    23581 gaagaaaatg aagatatgag taaggcttct aatataaata agagtaataa aaacattaat
    23641 aaagaggctg atgtaaaatc taaaaaaaat actgtagaaa atgaagatga taaagattct
    23701 gacttattta cagaggatga attgatgtct aaggcgaata ctttaaaatc agaaaaaaaa
    23761 actaaatctt ctatacaact atataagaaa gttgcagata taggtgaaaa taaagggaat
    23821 acatctgaag caacttatca agttgctatt ctcagtgaaa agttaaaaga ctataaaact
    23881 gctgaagaat attataaaat gtatgtagaa aattactcag aaaaagatgc ttattttgat
    23941 gaatcttatt ataatttagg gatgatgtac tataacaatg gtgatttaaa aaattctaaa
    24001 ttgacattaa agaagttagt taataaggta ccaaatagta tgtataataa ttcaaaagta
    24061 aaagaaattt taaaagaaga atagttaaat tagataagaa aaataaatta ttttatttta
    24121 aaataatatg attataatat attgacatac cattttaaaa aatttaaaat attaattata
    24181 aaatgaggag ggataaaaaa tggaatttga aaaagggagt atttcaattc atacagaaaa
    24241 tatttttcct ataattaaaa aatggttata ctctgataaa gacatattta taagagagct
    24301 tataagtaat ggatgtgatg cagtaagcaa acacaaaaga cttgtatcac taggagaaat
    24361 atcagaaaat aagtcatcag attataaaat tacagtatct gtaaataaag gagaaggtac
    24421 acttaaattt attgacaatg gaattggtat gactgaagaa gagataaaaa aatacataaa
    24481 tcaagttgct ttttcaggtg ctgaagattt ctttaataaa tataaggata agatggaaga
    24541 gtcaaatgac ataataggtc attttggatt aggattttat tctgcattta tggtgtctaa
    24601 aaaggtacaa atagatacac tttcatatac agaaggagcg actcctgtaa gatggatatc
    24661 tgaaggtggt acagaatacg aaatatctga atcagatgct agaaatgata gaggaactac
    24721 tataacacta tttattgatg atgatagtaa agaattttta gatgaattta ctgtaagagg
    24781 aataataaat aaatactgtt ctttcttacc agtagaaata tatttagaag atgttgaaag
    24841 attagaaaga gaagctaaag aagcagaaga aaaagctaaa aaacaaaagg aagatggaaa
    24901 agaagagatt gtagatgcaa aagtaattga gcctttaaac gacactaatc ctctatggtt
    24961 aaaatctcca aaagactgta ctgatgagga gtataaagag ttttatagaa aggtattcaa
    25021 tgtatttgat gaacctttat tttggataca tttaaatgta gattatcctt tcaatttaaa
    25081 gggtatactt tacttcccta aattaaaaaa tgagtttgaa ctgactgaag gaaaagtaaa
    25141 attatacaac aatcaagtgt ttgtggctga caatataaaa gaagttatac cagaattttt
    25201 acttttatta aaaggtgtta tagactgccc agacttacca ttaaatgtat caagaagttt
    25261 cttacaaaat gacagggatg taagcaaaat ttctaaacat ataattaaaa aagtagctga
    25321 taagttaaaa tctctttgta aaaatgaaag agaagaatac aataagtttt gggatgatat
    25381 acaaatattt attaaatatg gttgcttaaa agatgaaagt ttctatgaga aggtaaaaga
    25441 atgtatatta ttcaaaacta ttgatgatga gtatataaca atacaagatt atttagaaaa
    25501 atgtaaggac aaacatgaaa ataaggtatt ttatgtaagt gataaggaac agcaatctca
    25561 atacattaaa ctatttaaag aatacgactt aagtgctgtt gttttaaact catcaataga
    25621 tactcatttt atttcattta tggaatataa agagaatgga gttaagttta atagaataga
    25681 tgcagacctt tctgatgtat taaaagataa aaatgaaaat aaagatagtg aagaaaacaa
    25741 agaagagata gctaagatag aaggtttgtt taaagaagct gttggagaaa gagtgaagaa
    25801 ttattctgta gaaggattga aaaatgaaga cactccagct atggtattag tatcagaaca
    25861 atctattaga atggcagaaa tgcagtcaag atttgctgga atggatttag gaatgaattt
    25921 tgaggaagaa aaaactcttg taataaatga gaacagtcca ataatcaaaa aattggtttc
    25981 tcttaaagat gatgaggaaa agaaagataa gattacgctt atttgtaacc agatagcaga
    26041 tttagcttta ctttctaata aagaattaaa gccagatgag ttagattcat ttgtacaaag
    26101 aagtaataag cttatgagtc ttttgataga attataagat tgaatttgaa tacattgaag
    26161 caaatttata aaaagtattc aatatcagtg atgagaaaga agtgtctcaa aatgaacttt
    26221 tcagttcatg aggcgctttt tttatgtgaa attaaattta ttttaatcat ttcaacaatg
    26281 aaaaatatat ttatctctat tttgagacag accgttcttt atttttttat ctttggttaa
    26341 tttttaaata tttaagtagg ttctaataaa aaactctatt tagttaaata tagttaactt
    26401 gatatataat aagataatac atataattaa gtaaaagatt gttttttagt ttatgtttaa
    26461 atacataatt acactttgtt aaaaacataa aattattact aaagtagaaa taggaggttt
    26521 tagcatgaat tttaattata atttgccagt aaatatctta tttgggagag gcagaattaa
    26581 tgatgtggga aaagaagtat ctaaatatgg caaaaaagta ttgatagtaa caggtaaaaa
    26641 tagtacaaag aaaactggat tacttgataa aacaatagat ttactaaaag actctaaaat
    26701 agaatatgaa gtttttgata gagtagaaca aaatcctctt ataactacta tatattcagg
    26761 cgtagaaatt ataaaatcaa ctggatgtga ttgtgtttta gggcttggtg gtggaagcat
    26821 tatggatgct gccaagagta tagctttttc ttataaaaac ccaggagatt taaatgatta
    26881 tatatttgga attagacaag gtgaagaagc tttacctata atcttagttc caacaacatc
    26941 aggtacaggt agtgaaggaa attgttttgc agtacttaca aattcagaaa ctaaagataa
    27001 aaaatctata aagaagaata gtatgtatgc aaaagcatca ataattgacc cagaacttat
    27061 ggttacaatg ccaaagcata taattgcatc agttggattt gatgcacttg cccataatat
    27121 ggaagcatat ctttctaatg gaagaaaccc tcttgcagat gtacaagcta tttatgggat
    27181 tgaacttata tcagaaaacc taataaaggt atataatgat gttaatgatt tagaagcatg
    27241 ggaaaaaata acacttgcaa gtactattgg aggaatgacg attggtacag cgggtacagc
    27301 cttgccacat ggaatggagc atcctgtaag tggtcttaga gatgtaatgc atggaaaagg
    27361 acttgcagcg ttgactccta ctattataga aagttcatgg gatattgata ttgaaagatt
    27421 tggtaatatt tcaaaggtat taggaggaac ttgtgcagaa gattgttcag atgcaattag
    27481 aaatttcctt aaaaaaatag atttagatat aaaactaagt gatttaggta ttgaaaaatc
    27541 agatatagat tggttaactg ataattgtat gaaaatatca gttgcaaata ttaagagaca
    27601 tccaaagtat tttaataagg aacaaataaa ggaaatgtac cataagtctt tataatttga
    27661 ctttacatat ttagttttat ataaaaatta taaattaat
//
LOCUS       DAELSY010000037        27442 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.037, whole genome shotgun sequence.
ACCESSION   DAELSY010000037 DAELSY010000000
VERSION     DAELSY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27442
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.037"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..865
                     /locus_tag="KPG09_001447"
     CDS             <1..865
                     /locus_tag="KPG09_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0966066.1"
                     /translation="IIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRIN
                     HRLIRMYEVIQENRNSIAIEKADLQELVSDLSHQIKTPIANLKMINTTLLNQTFKTEI
                     QKDFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAE
                     KKNIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIRVATECWVMYTKID
                     IIDNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGK
                     GSTFSIFLPNK"
     gene            947..1612
                     /locus_tag="KPG09_001448"
     CDS             947..1612
                     /locus_tag="KPG09_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0966067.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGSLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            1625..4042
                     /locus_tag="KPG09_001449"
     CDS             1625..4042
                     /locus_tag="KPG09_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0966068.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNLSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKTISLQGNILSIIFIPLGAI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKQAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     SYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLIRNIEKKSL
                     TERLRSQ"
     gene            complement(4223..5014)
                     /locus_tag="KPG09_001450"
     CDS             complement(4223..5014)
                     /locus_tag="KPG09_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0966069.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(5047..5634)
                     /locus_tag="KPG09_001451"
     CDS             complement(5047..5634)
                     /locus_tag="KPG09_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0966070.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            complement(5811..7202)
                     /locus_tag="KPG09_001452"
     CDS             complement(5811..7202)
                     /locus_tag="KPG09_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBG0966071.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(7392..8690)
                     /locus_tag="KPG09_001453"
     CDS             complement(7392..8690)
                     /locus_tag="KPG09_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBG0966072.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSTERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKLLSSDLNN"
     gene            complement(9051..9926)
                     /locus_tag="KPG09_001454"
     CDS             complement(9051..9926)
                     /locus_tag="KPG09_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBG0966073.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIKEYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(10119..10805)
                     /locus_tag="KPG09_001455"
     CDS             complement(10119..10805)
                     /locus_tag="KPG09_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBG0966074.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(10854..11387)
                     /locus_tag="KPG09_001456"
     CDS             complement(10854..11387)
                     /locus_tag="KPG09_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG0966075.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDA"
     gene            complement(11431..12063)
                     /locus_tag="KPG09_001457"
     CDS             complement(11431..12063)
                     /locus_tag="KPG09_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBG0966076.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYNTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(12153..13232)
                     /locus_tag="KPG09_001458"
     CDS             complement(12153..13232)
                     /locus_tag="KPG09_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBG0966077.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(13365..14711)
                     /gene="pepV"
                     /locus_tag="KPG09_001459"
     CDS             complement(13365..14711)
                     /gene="pepV"
                     /locus_tag="KPG09_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBG0966078.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(14780..16060)
                     /locus_tag="KPG09_001460"
     CDS             complement(14780..16060)
                     /locus_tag="KPG09_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBG0966079.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(16187..16447)
                     /gene="hfq"
                     /locus_tag="KPG09_001461"
     CDS             complement(16187..16447)
                     /gene="hfq"
                     /locus_tag="KPG09_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBG0966080.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(16561..17496)
                     /gene="miaA"
                     /locus_tag="KPG09_001462"
     CDS             complement(16561..17496)
                     /gene="miaA"
                     /locus_tag="KPG09_001462"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBG0966081.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDIDAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKGKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTARWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(17534..19501)
                     /gene="mutL"
                     /locus_tag="KPG09_001463"
     CDS             complement(17534..19501)
                     /gene="mutL"
                     /locus_tag="KPG09_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBG0966082.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKDKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(19512..22355)
                     /gene="mutS"
                     /locus_tag="KPG09_001464"
     CDS             complement(19512..22355)
                     /gene="mutS"
                     /locus_tag="KPG09_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBG0966083.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(22518..23252)
                     /locus_tag="KPG09_001465"
     CDS             complement(22518..23252)
                     /locus_tag="KPG09_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0966084.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(23221..24234)
                     /locus_tag="KPG09_001466"
     CDS             complement(23221..24234)
                     /locus_tag="KPG09_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0966085.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(24256..25026)
                     /locus_tag="KPG09_001467"
     CDS             complement(24256..25026)
                     /locus_tag="KPG09_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0966086.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(25001..25291)
                     /locus_tag="KPG09_001468"
     CDS             complement(25001..25291)
                     /locus_tag="KPG09_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBG0966087.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(25422..25521)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      26141..26230
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            26283..26459
                     /locus_tag="KPG09_001469"
     CDS             26283..26459
                     /locus_tag="KPG09_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966088.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(26574..26930)
                     /locus_tag="KPG09_001470"
     CDS             complement(26574..26930)
                     /locus_tag="KPG09_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0966089.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKDVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
BASE COUNT         9165 a         4502 c         3073 g        10702 t
ORIGIN      
        1 aataatattt ttacttcata aaaaaatagt tatattttgt tctaaaatat gtcacacttt
       61 agatagtatg atgaatagtg aagacaatat tgaaattgaa ttagaagaag aaactatttt
      121 atcacgtatc aatcacagat taattagaat gtatgaagtt attcaagaaa atcgcaatag
      181 tattgctata gagaaagctg atttacaaga actagtttct gatctatctc atcaaattaa
      241 aactcctatt gcaaatttaa aaatgattaa tactactcta ctaaatcaaa catttaaaac
      301 agaaattcaa aaggattttt taatggatat ggaaaaccaa ttagataaat tagatttttt
      361 aatgcaatct atggtaaaaa cctccagact agagacaggt ataatcacac tatctaaaaa
      421 gaaaaattca atttatgaaa caattgctac agcacttagt ggaattttgt ttgatgctga
      481 aaaaaagaat attgaagtta cagttgattg tgacccaaat ttatatataa ttcatgacaa
      541 gaaatggact tctgaagcta tttttaatat attagataat gctgtaaaat acacaaattc
      601 taatggtaag atacgtgtag ctacagaatg ttgggttatg tatacaaaaa tagatattat
      661 tgataatgga aaaggaatat cagaaagtca tcaagcagaa atctttaaaa gattttatcg
      721 tgaagaagat gtacatgaca tagaaggtat tggaataggt ctatatcttt caagaaaaat
      781 tatatctctt caaggtggct atattaaagt tacatcagat attggaaaag ggtcgacttt
      841 ttctatcttc ttacctaata aataaatttt taaatatttt atttgtaatg attctgcaac
      901 attttgattt tatactgtat ttacaagatg ttaaggaggt attattatga gtattttaca
      961 agtaattaat ttaaagaaat attatggaca aagttcaaac attacaaaag ctcttgatgg
     1021 tgtaactcta tctattgaaa aaggcgaatt tatttctatt gtaggtacat ctggtagtgg
     1081 caaatccaca ttattaaata tgatgggagg attagataat cctacatctg gaaaggttat
     1141 tataaagggt aaggaattat ctactatgac tgatgaacag cttacaatct ttagacgcag
     1201 aaatataggt tttatatttc aaaattataa tcttgttcct ttacttaatg tatatgaaaa
     1261 tattatctta ccaataggat tggatggaga gaccatagat aaggatttta taaatgaaat
     1321 tataaatatg ctttctcttg aagaaaaatt atatgatatg ccaagtaatc tttctggtgg
     1381 tcaacagcaa cgtgttgcta tagctagagc acttgtaaca aaaccagcta ttatactagc
     1441 tgatgaaccc acaggtagcc ttgatagtag aactagttca gatgtactga gtttacttaa
     1501 agtgacaagt aaaaaattca accaaacaat tgtaatgatt acacacaata atgaaattgc
     1561 acaactaact gatagaatta ttcgtattga agatggaaaa attgttaact aggaggtgta
     1621 gtatatgttt agaaataata atcgcacatt tataagaaaa atagctttaa atgatttaaa
     1681 aattaataaa cttaaaacat atctttcagg tatcattata atgatttcta catgtttatt
     1741 attaacagtg accttagttt catataatgc ttctgtagat ttagttaatg catctccata
     1801 tcatgctata tataaatctg tagatgaaaa agcaaaaaat atactatata aagataaaaa
     1861 ccttagtggc gtaggaacct acaaattaat tggaagtaat aaaaagacag actgtattat
     1921 gtcgattgtt tatgcagatg atacagcaat taaacttatg aattttcaac cattgaaagg
     1981 caaactacct acaaataaaa atgaaattgc tatttcagaa aaatatttac aggaatttgg
     2041 tttagataaa gatattggag atagcattaa attaaattat tatgatgata ttacgaataa
     2101 agaagttcaa tgtgatttta ttattgttgg atttctagaa aattattatc aagataatgc
     2161 caagcaatac tatacagtcg tttcaaatga ttattttaag aatatagctt ctacaccttt
     2221 acaaaataaa agtaatactt ttgacgagtc aagacctgat actgtagatg tattagttaa
     2281 actatcagaa gaaaatacag agaaagattc agcttcaatt aagacacagt taaagaaaat
     2341 tggtttatct ttaggtatta agaaatataa tatatactta aatgataact atattgaatc
     2401 aaatttaatt gatggtgagc agattattac agttttagta gttggaatag ttgttctttt
     2461 ttctagcgtt tttgttatat atagtatttt ttatatttca gttgttaatt cagttcagat
     2521 gtatgctaag ttgaaatcgt taggtatgac ttcttttcag ttaaaaaaga ctatttctct
     2581 acaaggaaat attttgtcaa ttatttttat tccattaggg gctatagctt cttgtataat
     2641 tgcttacata attcaaccat tagcatggca aatgatagct gatttattta taattttaat
     2701 tttatcactg gtaatgtttt taactgttag aatttcttta tttaaaccag ctagaattat
     2761 ttctaaaata tctgctattg aagccatgca atatacagaa actaaattta gaaaacaagc
     2821 aaaaaatttt agttatataa atataaaaaa tctagcatta aagaatatcg agtcaaatag
     2881 aaagaaaaat ttgattgcat taatatcgct aagcattagt ggagttttat ttatttcaat
     2941 cgctaatctt gctaattcaa tagatttcaa aaaacaacta tctcaacaat ttgtctataa
     3001 cgaagattat ataattagca ttaagagtga taacttatat gagcatatta cagaaatcca
     3061 acaaaataat ccactaacag attctcttaa gaatgaaatt aaatctattc atggagtaaa
     3121 aaaattaatt gaatccaaat caattaaaag taatattata gaaccttaca tcaaagatag
     3181 agacgaaaaa caatttatta ctctaattaa aggtattacg cctgaacttt caaaatcact
     3241 tgaagaatat attgtcagtg gaaaaataga ctatacacat ttagattcag atagtttaat
     3301 tataaataaa tatagaacaa aatcttatgg attaaattta aaagttggag atacagttac
     3361 atttaatatc tttagtggaa atgatattat taaaaagaaa atgaaaatta taggaatcat
     3421 tgacaattcc aatacaggag gtatgttttt tgcttcagac aaaactttag aatctctaac
     3481 accatataat acaaatttag atttttctat tttaatagat aaaaagcatt ctaaagaagt
     3541 tgaagaaaag ttgttaagta taattcagaa aaattcaaat ttaatgcttt attcatatga
     3601 agatgattat agaatgatta ctagagcatt tcaatatata attatagctt catacatttt
     3661 tgttggttta atttcttgct ttggtatact aaatatgacc aataccctaa taaacagcgt
     3721 tttaattaga aaaaaagagt ttgccttact tcaagccgtt ggaatgacaa gaaagcaact
     3781 gcgaaatatg ctttatagag aaggtctaaa cataagtatc aaagctatat gtacttcaag
     3841 tatactaggt tattttggtt caaatctact ttgcactttt ataaaagatg taattagatt
     3901 agactttatt aattttaaat tttctatatt tactatttta attttttcat tcgtattaat
     3961 tggtatacaa gttttagtga ctgaattact tataagaaat atagaaaaaa aatcacttac
     4021 agaacgctta cgttctcagt aagaatgtat ataaaaaata accatttttt atacaagttt
     4081 taacataatt ttttaaaagc taaaaggata gaaaattttg actttacata attttctgtc
     4141 cctctacttg tttttatttt atacagatac acttccttat atctccaagc aacatcacca
     4201 gagacataaa caagcatatt ccttattttc taaatatgtc aattgcatga cttgtaactc
     4261 ctactaaatc ttcacgttca caagtagcaa ggtgatattt taaatataac gcaaatgcat
     4321 catcatccat agagtctact gcttcacgca tatacaaagc caaaccatct gatgcgacat
     4381 aatgcagtct agttacatta aatgcagaca tcaaatcatc aatgttttct ttacgtacaa
     4441 gctcaaacaa atcctttggt tcagacttag cagcaaatgt ctgtggatca atcaaacctt
     4501 tttcaatata ttcagataca ttaatatttc ctctatggaa cccctcgtca atgagacagc
     4561 catcagatat aacgtatgca gcaaaaacca caccaccagg ttttgttaca cgaattgcct
     4621 catgtaaagc ttgttgttta tcttctttgt tataaagatg atatagtggt cctaacagta
     4681 acgtaatatc atatttatta tctgggaaaa cagataaatc catagcatta ccctgagtaa
     4741 tgcttatatt ttcatttgat tgagtatttt tatggaaaac ctctatatta tgttcgacta
     4801 attccactgc gtcaacatca tatccctgac gtgccagtgt gtgagaataa cgaccagttg
     4861 cagcgccaat ttcaagcaca caatcacctg attttatgta cttatcaatg taatgcatag
     4921 tagtgagaaa ttccaccatt ccatgtttta atgcaagtcg actatcttca tcataagtat
     4981 tataaaagtc aattaagtat tgttttgttt ccataatctc acctcttcta tattagttaa
     5041 taaacatcaa atagacaaat tccaatctgg tttttcttgt cttataaaag ctgttaatat
     5101 atttttcaac attttttcag cactatatct atctaatgtt aatttttttg ttgctgttaa
     5161 gacagctatg ccatgagtat aaagaaataa atctaaaaat attacttttg cacattccaa
     5221 ctctattcca atgctttccg aaaaaagtct tgcctttttt tcattgtcaa tttctttata
     5281 aaaatcattc gcctctttca tcatcaaatc taaatcattt ataaataaca acttaaataa
     5341 atacgtttct tcttgagcaa attcaatgta tgaaagcgga agcagtaaat aagggcttac
     5401 acttttagaa ttacgataat taacaacata ctgattataa tattcatatg caaaagcaag
     5461 aaattcattt tttaattcat tcatgttttc gtaacaagta aaaattggtc gagtggaaca
     5521 ctgtaattta cttgcaatac tcctcgcatt tacagcttca aatcccactt ctcttgtaat
     5581 atccaagact gtattcaaaa tcatttcttt tgttatcttt actttaggtg gcatatatgt
     5641 tcacctctcc attaaatttt tataaaacac atatgttata taactatagt aacatatcaa
     5701 gaaattatgt caagaaaaag ctttgcattt ttttgcaatt cctaaaactt ataaataaaa
     5761 catgagttgc aattaatcta ttaatagata acagcaactc atattttcct ttaaatactc
     5821 ttctttttgt ctaatatttc atatacacaa tcttctattg aattaatatc tttttctttt
     5881 ctttttttag aatcaattaa aatgaaatac agtataaaac ctacagcaag tccccatgtt
     5941 ccgcctttag cagcaattac acctcccata agtccaatca atcccttctc aacacctgta
     6001 ttacatagct ccattgatag ctttgtacat aaatatcctt gaactaaaag tgttactgac
     6061 aatgctaatg gtataagagg tgtagcaatc tgcgcaattg gataaaaaca gataaaaatt
     6121 gctgaaaccc atactaatgt acccatgcca ctaaaaacag actgcatggc ttcctttcca
     6181 accttgtacc tttgagcaac acttcctgta agtgtcgcag ctaatggacc gcacattggt
     6241 atatatggag aaaagattgc cataaaaata tttcttatcc cacttataac attagaccta
     6301 tttgaattga aatctatata ttcatctttt ctattttcac tttcagaaag tactaagttt
     6361 tcacctgtta taaaatcacc aaaggcaatt atgtatatca taaaagccat tggtatccct
     6421 tgtataaata acattacact tggaaaacct attccaaaag gagatactgt atttatcaga
     6481 tttttaaaat ctactatgta aactaaattg tcaaaactaa atctaggaat ttctatttct
     6541 ttactaaata tacctactat taaactcact aaaatagctg atactacacc atattctcct
     6601 aaatgaaata aaaatttatt cttaactttt agagtatcaa atctttcaga aaacattaca
     6661 aaacaagcaa ccaaaattcc tactgttata gatataggat aagaatcaaa tctcccttta
     6721 ggattaaact ctgatattat agtcgatata ccgacaccca ttagtattcc acctttaata
     6781 gaatttggta tcttactaac taactttcca gctacaccag ttatacccat taaaataaat
     6841 attattccta aatctatttg taaagctgtt aatgcttgta ttctttcaat gcccatgctg
     6901 aactttaaca aatatgcagt tgtaagagtt atagaagctg taatccagcc aggtacaact
     6961 gggtctccca taaaaacatg caaacagtat aatattgagt taataatagc catactccat
     7021 gccacttcat agggtaatcc caaaatttgt gtactaaggc caattgtgcc taaacttgtc
     7081 atacttgata ttataccttg aatagcttct ggtttctcaa atctgtaatg tataaatgga
     7141 attcttatct taaacattcc aaattttata tgaggttgtt cccttctagc gagtctttcc
     7201 ataacttcac tccacctttc aataactctt ataagtttac aactttataa ttgtttataa
     7261 attgtcaatg tcattaaaat tagtatataa catgcaaatt atataaacaa taaattaaat
     7321 ctataaaatt gatagatttt gtgcatatat agaaaaaccg cttcattttt agttacttaa
     7381 atcacttaat attagttatt taaatcactt gataaaagtt ttttataaaa ttcaatagtt
     7441 ctattccatg attcaaactg tccttttgaa tttccttcta cagttccacc tgaagaaaaa
     7501 atccctccac tcatactcat acataaagtc tcctttagag gcataatata tggaattggt
     7561 aacggatgac ccatattatt gtatgttaat aattctacgt tatatggata attattttct
     7621 tcaaagattt ctaatgcctc taaacaagct tcataactat tccatatgtt atcttctttt
     7681 ccagcaatca taagtatatc tgctttggaa ttttctattt ttattctagc ctcttttcta
     7741 ttctctgctc ttttaagact tttcttataa gtactattaa atccaaatgg aatacctttt
     7801 gatatatttt ttttctgttc ttcaaagaat atatcattgt ctactttgat atatggaaat
     7861 ggctctcctt tatatgacca tgatgaagta tcatttccac tcatcaatcc attaagagct
     7921 tggaaacaat aaatgtgagg ttgtgatact acaacctttt taatttgttc atatcttgaa
     7981 gcaagtaaaa aggccaattc tccacccttt gatgtcccat gtacaaatat ttctttagta
     8041 ctggtaattt cattttcact aatccactca aatacttttt caaaatattc taatggaacc
     8101 tcttctaatt tatctggtaa cccttctaaa gcaaagtatg caactgttag tacattgaat
     8161 cctctagatg caagtggagc tgctaaaagg tctagagcgt ctaaatttcc atcagaacca
     8221 cctagcatta aaattgtttt attattagaa ttttgtttat aaaataattt tcctataaac
     8281 tcatcagata tgtattttgt ggtaatatct tctgatttaa atattctctt taattcaact
     8341 tcttttcttt cagtagaagt ttcaaatatc atattcataa ttattggttt atcaatagat
     8401 atattttctg caaaattttt accttcagtt ttagattttt ccagcgaata aattaacccc
     8461 atacaattat cagccttata agtaccttct ataggttcaa ctaaatctaa atctacttct
     8521 cctttttcat ttgaacaaaa cactccataa gatgaaaatt cttctccctt gcaccatgga
     8581 aattccattt tcacacttat tttaagttta gtatttggaa ttaagtctga aaccataatc
     8641 ttaagtcttt catccacaaa tgattctaca ttgtatacat ttactttcat aatctatttc
     8701 tccttataat aattatttat gctttatata taaaacaact ttatctaata caacataaat
     8761 aaatttttaa attaagacac catattgact atttcatttt atcaacatat ttcatttttg
     8821 tcaatatatt gtctttttta gtaatttaaa attatgtagt ctatatgaaa tccagttgat
     8881 ttttggctaa ttaaagatag gaatgctatt aggtttaaaa tatatctatt caaaataaaa
     8941 aaccactcat tttaagaatg gtttttaata taaactattt gttattaata aggatatttt
     9001 tttatttaaa tttatataat tgaacatcta atatttaatg tttaataaaa ttatgcattt
     9061 gcaaaagtat atatggcatt aactcttgct acatataaat tctcatcact acatccaata
     9121 tatctagcat atttttcaat cttttctttt gcattttcaa catcattttc atcgctctcc
     9181 attaactcat ttatataaat atccattaat gacgctttat attcttttat atcatagcct
     9241 aaaaattcag tcaatctctc aatttcctta ctaaaatctc catcttcttt taccaaaaat
     9301 tcgtatacat cttccctata tggatttaaa gttattattt gttttactac tttgcattcg
     9361 tcagccttcc caataaaacc atctttataa ttattaaata aggcattaga tttttttata
     9421 gattcttcat caatataaga atgtaccgca gttatacgat tttctataag ggcatctaca
     9481 acagcataat gaattttaaa acaatcattg tataaataat tgctcatttt ctttattgtc
     9541 gattctattt ctaagaagct agcgtgccca ttttttttat ttttatttgg tataataaaa
     9601 gttttattca agttattaaa aagtctctta aaatctaaat atttttcaca ataaacgctt
     9661 ttaaaggtat cgatatctat agttataatt ccaaaactta ctatagtctc aattcctttc
     9721 ttaaaagact cttctatata tttttctcct aaacttaaac ctctatcaga tatttgcttt
     9781 agattttgaa aactatttaa acaatctctc tcaaagtttt tagatatttc taaagcatat
     9841 aatttaaaga ttttccttat cttattataa ttattttttg tatcatcaaa tctgataata
     9901 gctccattta catcaaaagt aatcattgag ttttaacctc cccattagtt tcatcaataa
     9961 aataaattta atataaactt tataatcata atattaaact tatttatact ataattatta
    10021 atttgatgat aaaagctatt ttccttctaa ataaaaaatt tgtctaaagt ttctaaaaaa
    10081 tttagacaaa tcttaggtta ttattcttta aattattttt aaattattcc aaatacacca
    10141 aataatattg gtataaatat agctggtaac aaattagcta cttttatatt cgttaattta
    10201 agtacattta gaccaagtcc tataattaat aaacttccag ctgctgtcat ttctgtgact
    10261 acaggatcac ttaaaaatcc atttaaaaat gatgctccca cacatataat accttcatac
    10321 aaaaatacag atattgcaga aaacattaca cctataccta aagaagcagt aaatataatt
    10381 gaagctattc catctataac agattttaca aataaagtat catgacttcc tgtaagacca
    10441 ctttctaatg aaccaactac tgccatggct cctatacaga ataataaact agatgatata
    10501 aaaccctctg ctatagaatc ttttttattt cctttagaaa atttactttg taaaaaagca
    10561 cccaacttat ttacccattt gtcaatatct attaattctc ctatcagagc acctacagct
    10621 acggatatta ttgtcactaa tgtgtttttt ccttctagtg ctcctgatat accaatgtac
    10681 aatacacata aggcaattcc attcattata gtatcactta ctctttgagg taatcctcct
    10741 tttattataa gacctactat acatccacct gcaattgcta gactatttgc tattactcca
    10801 aacatttttt ctcccctcga ttagctaatt gaaatttatc ctattattaa aacctaagca
    10861 tctatacttt gtattggata ttttaaatat tccaaaaatt cttcaaaacc ttttctagat
    10921 atatttacag ttttagtatt tatgttagga tgaaaattta agtaatcttc tttaattaca
    10981 tctttatcta tgtaaagctc aacttttctt tctgtatcat ttattaatga aaaaggattt
    11041 acacttccag gaagaagttt aagtacattg tatagtcttt caggtgaagc aaacgataat
    11101 ctagaacttc ctatcttatc tttaagtgag tttaaatcaa cttgtttttc tcctttaact
    11161 acaataagat aatacttatt tcctttggca tttcttaaaa ataaattttt acattgacac
    11221 ccctttgtta ttccatctaa aatgactaat tgctctgctg tatatacagc ttcatgctct
    11281 actacatcat attcaacatt taatttatcc aaaatatcat atattttagt ttcattcatg
    11341 ctagattcat tttcatcttg ttctttttta ttatttaaat cactcatact tttctcctta
    11401 aacacttcat cataatatca ttgtgaggta ttattctact atagctattg ctcttactgg
    11461 agctccatca gcatcattat acttaaatgg tgctgctata aatagaaatt tttctggaac
    11521 attttctaaa tttgtaagat tttctatgat tatttttcct ttttcaaata aaatatgatg
    11581 cacttcaaat actgatgtgt tatatctatc cactgatagc atatctattc ctatgccttt
    11641 tatattagta ttagctatat agtttgcagc ttcttttgtt aatgtaggat aacctacata
    11701 gtactgcttt ttatcccaaa attttgacca acctgattta aatattataa aatcactatt
    11761 ttttatttca tcttcatatt tttttagata ttctaattct atattctcat ttcccttttc
    11821 aagttccaat acataagctt tcccaacaaa attttctata tccaacttat ctagggtctc
    11881 acctttagta tacatatgtc ttggtgaatc catatgagtt ccattgtgag aaaatacact
    11941 aattaatgtt tcttgataac cattttcttc aataatagct actttcttga tatctggtct
    12001 attaggctct gcatatacag gcatgtcatt atgtgtcaca tgagttaaat caaaaacctt
    12061 catctttata taccccctat gtttttattt tctattatat acctaaattc attttatttc
    12121 tatcagattt tattccatta tataccatac tactatatat tttttataaa cctcaaggga
    12181 tttaccgaaa aatcttggaa agaattatca gtaaatataa catcttcacc caaaaaacgt
    12241 ttataccttc cttcttcttt ctttaaagta attctctgtt tttgtgaatg attattaaaa
    12301 accattatct taagaggagg tttttttcta ttagtctgca ctctttttaa catgtatttt
    12361 atgtgcatat cagcttcagg aaatgaatat ccacaaaata ttaaaaggtc agtatctctt
    12421 aaagtttttt ctgtttcatt ccaaacatta cttaaaaata tgtttgacat attcttaaaa
    12481 tatgttggtg gcactataat aggttctgtt aactcaccac actctaaaca ttttgtctct
    12541 gaactatttt caataagctt catgacacca ccttcatgtg gtgtaagagt tacactatta
    12601 caaatggggc aataaagcca attcaatgac ccatgtatct tatataattt gattaatggt
    12661 tcttttggtt ttctccaatt taactcctct ttaaaatttg taaactccac accataatca
    12721 agcataattg gcaaatcatt cttatctctt aatccagtaa ctgtattgtc tatatgtata
    12781 tcataatttg cactaataaa agtagtatca agaagcaaat cattttccaa aagattttca
    12841 actaaaagtt tatgatattt attatctgtt ctaggtgtat tatgaattgc ttcagccatt
    12901 agcagtatta aatattgtct taaaaaacgt atactatcac ttctgttatt tagattttct
    12961 agtccaaaat ttctaaatgc ttcttttctt tgttctgcta agtctaaaag acctaaaact
    13021 tcttcaaagg taggaaaatt tgcattatct atattatctt ttactatatc aatattgaac
    13081 atctgtttaa agaaaatata caattctcta ttcattttgg tattataatt tgtgggattt
    13141 agttttttaa aatattcgct aaaaagttca ttttgaatag gtagattctc tgctgctgaa
    13201 gctcctgctc ctaaaaatat tgctattttc atatagttcc tcctaatata aatattactc
    13261 ctatttttat atccattaaa tatccatatc atttatttta tgatacaaca ataaagctgt
    13321 cttttactag aaaaaatcct attacaagac agccttatat tatattaaat ctttaataat
    13381 tcgtaaatag aaagagcata tatttgagta gcttttataa tatcctctac tattaagtat
    13441 tcattagctt ggtgttctgt ttctggtttt cctggaaaaa tagctccaaa tgcaacacaa
    13501 ttatctaaag ctcttgcgta tgttgctcct ccagatgaaa gtggtgttcc atcaagacca
    13561 gtttcttctt catatacttt tctaagtgtc ttaacaagaa gagtatcttc tggaacatag
    13621 attgaatcca agaaatcata ttcttcataa tttaaattgt atttatctgt cattttcttt
    13681 aactctttta caaaatcatc ctttttgtat gttactggaa tccttacatc tattcctgca
    13741 aattcttttt cgttatcaat agttactcta cctatattta ctgttagttt acctgataca
    13801 tcatccttgc aattaggaat tatattgttt ccattagcat cttcaccaat aacttctgct
    13861 aaaaacttaa ttatattaga atctatacct atattattaa gagctataca aagtctagcc
    13921 actgcattta taccagtatc acttgctgct gaatggacac tttttccaat tatacagatt
    13981 ttattacctt caacagtata ctcaaaattt aatttatcaa gttctttttt taatttatca
    14041 gagtattttc caatatagat tgctttacct ggaactgcat ttaaagcctt tccaactgac
    14101 aactctatat cacttttgct atcacaagtc aaatgaactt gtaaaagacc tttctctgca
    14161 tttgttattg ggaatctaga atctggagta aatccatagt ttggtatttc ttcattattt
    14221 tctttatatt tatttataca tctccaaaga ttttcttcat cagccccaaa aatgaatctt
    14281 atcctcttat taaagtctac atttaaatcc ataagagctt tcactgcata aattgctgat
    14341 attgtaggtc ctttatcatc ttgtgttcct cttccataca atttaccatc ttctaaaaca
    14401 gcttcaaatg gagggtaatt ccagctttct aaatcacctt ctggaaccac atcaacgtga
    14461 ccaagtatac ctatcatttt ttcaccttgt cctatttctg catatccata atatccatct
    14521 ttgtatactg tcttaaatcc taaagattta cataagtcta atatacctct taaagcttta
    14581 tctacatttt caccaaatgg gcaattttca gttgcttcag atataacact aggtattttt
    14641 acactttctt gtattgaaga aatcatttca tcttgtagag aatttacttt ttctttaatt
    14701 tgctctttca tattatcacc cttcaatttt catcataata tactaataaa tattttgacc
    14761 tttttaaaaa aaaatatgct tattttttca gtttttttat aaattcaatc gcttttaaag
    14821 tccccatttt tgaatggtca aatgtaagtc caccttgaaa ataaacatta taaggaggtc
    14881 ttattggagc atctgcactt aattctatag aagaaccttg tataaatgct cctgctgcca
    14941 ttatgacttc actttcgtat ccaggcatgt cccaaggaac aggttttaca taagaatcga
    15001 ctggtgcagc ttcttgtata ccttcacaaa aacttattac ttcatcagca ttattaagtc
    15061 taatacattg aataatatca cttcttaagt catcatattt tggtaataca tcatatccta
    15121 atttttcaaa agctcttgag caaaagatag ctcccattac agcctgagat actatataag
    15181 gcgccaagaa aaaaccctga agtacatttc tagttgttcc aaaagtaaga ccacattctt
    15241 ttcctattcc aggagaagtc attctataag atataagctc aatcaaatct tttctaccag
    15301 ctatatatcc acctgtcaat gcaagcccac caccaggatt ttttattaaa gagccagcca
    15361 taacatctgc tccaacatca gtaggctctt tggtatctaa aaactcacca taacaattat
    15421 caaccattac tatagcttct ggtttaacag actttattac ttctatagct tcttttatat
    15481 cactaattga aagtgatttt ctccaagaat atccttttga cctttgtatc ataacgagtt
    15541 tagttctatc atttattttg tttttaattc cttctaaatc taagtttcca tcctctaaaa
    15601 aatctacatc atcatatgta acaccatact ctttcaatga acctttttct tctcttatgc
    15661 ctataactcc ctctaaagta tcatatggtc taccagtaac agataaaatt tcatctcctg
    15721 gtctaactat accttgtata cataaagtaa gtgcatgtgt accatttaca attattggtc
    15781 ttacaagagc atcttctgta ttaaatactt tagaataaat ttcctctatt ttttcacgac
    15841 caatatcatt atatccatat cctgtagtcc agttaaagtg catatcactt agtttagatt
    15901 cttgcatagc ttttaaaact ttatattgat tatattctct tatttctttt atttcttcaa
    15961 acttatcttt tatttcttcc attatttctt gcgatagttt aaaagtatca tcatctattc
    16021 catagtagtc ttttaataat tcttttgtct cattaagcat tttatcacct gtctctttat
    16081 tattttattt aaacatgtaa aggagactat tttactaaaa caaataaatt atagtttata
    16141 agttagtata agtctcctct caatcatctt aaattaaatt aattatctat ctgttgttat
    16201 tattattgtt gttttggttt tgatttgtta gattaatata ctttccaggt tgtatagttg
    16261 atacagcatg tttataaatc atgttttgtt gtctattatc cccttctatt aatattatat
    16321 aactatcaaa cccctttaca agccctttaa cttgcactcc attaactaaa tatatagtaa
    16381 caggtatcct ttctttcctt gcattattta aaaacaaatc ttgtaaattt aaaactgtat
    16441 ttttcattag atttgtaccc tcccaagatt aaattgaact tctaaaatat tcctacctta
    16501 tattctatat aagttatgac aaattttcaa tattaagttg tatacttaaa aatttatata
    16561 ttattttata gaatctttaa tatataagat tatttcattt tttaattcgt ctatattttc
    16621 atattggtct aaatcaaacc atcttgctgt tttatatctt ttaaaccatg ttatctgacg
    16681 ttttgcatac cttctagaat ctcttttaat tatttcaata gcttcttcgt atgtatattc
    16741 tccatccaga tacttcaata tctctttata acctatacct tgcattgata tcatatcttt
    16801 tttaaatccc atacttaaaa gtttttttac ctcttcaacc aatccatttt taatcataat
    16861 atcaactctc atatttattc tttgatacaa atgttctcta tctctattta gaactattat
    16921 tattggttga tattcttcat taaattttaa atcacttgaa aaatcattca ttttcttacc
    16981 acttagacaa acttcaagag ctcttatgac ccttttagta ttgtttttat ggattctatt
    17041 agcagattct tcatcaagtt cttttaactt attatgcatg taatctattc ccttttcagc
    17101 aagctgtttt tgaagttctt ctcttaattc attattggca tcagattgtg caaaatccat
    17161 attatatatt aaagagttta aataaagtcc tgtgcctcca gttactaaaa cacattttcc
    17221 cttttcattt atttcatgta tatatttttt tgctctttgt tgaaactctg aaactgaaaa
    17281 tggatagtca ggcttaactt catctataag gtaatgcttc actccttgca tttcttcctc
    17341 tgtaactttt gcacttccaa tatccatata ttcatatatt tgcatagaat cagctgaaat
    17401 tatctctgca tctatatctt ttgctaattt tattgacaaa tctgtttttc caacagcagt
    17461 tggacctgta agtattataa gtggtatttt tttcataaca ttcctttcca tatattttaa
    17521 atatccaaat attttacata attcttttaa acattttttc tatttctgtc ttagatattt
    17581 cgaccattat aggtcttcca tgagggcatg taaatggatt ctcacacttt tctagctgtt
    17641 ccaataaact ttttatttca atatcgtata ttttatcatt tgcttttata gcagacctac
    17701 atgccataga tgcaaatctc tctcctttta aatcataatt acttgtaatt tcttctatat
    17761 tatcaattat ttgaagtata aatttctcag tttcaggtac tccaaatata gtaggaacac
    17821 atcttaccat tatatgatta ttgccaaaaa tttctagttc aaaaccaaat ttcataaata
    17881 actccaagtt attttcaatt tgaagcatat ctacatttga tacttcaata accactgggt
    17941 ctaaaagtat ttgcatattg atatcttgtc tatagaactt ctccatatat ctttcatata
    18001 gtattctttc atgtgcagca tgttggtcca acaaatacat agaatcgtct ttgctcaaaa
    18061 ttatataagt attaaataca actccaatta cagaatatcc atataaggaa aacttgcgtt
    18121 ttgaactaca caagtattct tcttcactgt ccttaatact atctcctaag taataatttc
    18181 cttcattttt aattccatca acttgaaact cttcttgtat attatcattt gcactatctt
    18241 cacttagata attaatgttt tttacatcat cttctacact tgcatttagg acttcactta
    18301 ctgattgaaa ttcatttatt ggtttttcac ttgataaagt cacaacttca atgacttcat
    18361 ccttatcttt agtaatatta ctatttttag gagtagattc taataaatca ttatttctta
    18421 atttataatc tgtacttttt tctctactgt taatagcgat tctaggttgt gtttttttat
    18481 cactataagt agcatatttt ccaataagat ttgagtgtat taatttaacc tttaaaaaat
    18541 ctctaagttc tatatacact tcttgttctt tttcaaattt aatttcaagt ttattggggt
    18601 gtatatttac atctatacat gatgggtcta cttctatatt taagaagcac actgcatgtt
    18661 tacctattgg aattatagac ttataacttt ctgttattgc atctatgatt attttacttt
    18721 ttacaaatct tttatttata tagatatgtt gtaagttttt atttgaacgg tatatattgt
    18781 tattacctat atatccattc attttaaagt gattgcactt aaactctaca tctattatat
    18841 tttccgttat ttcttttcca tatatacttc taatagtgtt tacaagtttt ccatcccctg
    18901 gagtatttaa catctgttta ttgttatttg tatatttaaa ttgaatgttt ggattaccta
    18961 ttgcaagttt atttatcaaa tcacttatgt ttatagtttc tgcatgagta gattttaaaa
    19021 atttctgtct tgctggtgta ttaaagaaaa tatcttttat aatgatggta gttccattag
    19081 tagaacctat tggttctttt gatattattt ttccgccttc aacatatatt ttagttccta
    19141 ttatttcatc tttagtttta gtcgtcattt ctaattttga tacagctgaa atagatgcca
    19201 atgcttctcc tctaaaacca agagagtata aatcatacaa atcatcaatt tttttaattt
    19261 tactagtagc atgtctcaaa aatgattttt caacttcact tgaaggtatt cctattccat
    19321 tatctgtaat cttaattaga ctttttcctc catcaattat atctatggaa attttatttg
    19381 cacctgcatc aatggagttt tcaataagtt cttttactac agaagaaggt ctttctacaa
    19441 cttcacctgc tgctatctta tttatagtca aatcatctag gatgttaatt atatttttca
    19501 tttttacacc tctaagattt tatttctttt gcttttcttt gaagattata aagagagttt
    19561 atagcatcta atggagtcat atttaaaata tccagattta aaatttcttc tgatagtata
    19621 tctctattta cactatcaaa agagatttga gttaaggcta cttcttttac agaatcttct
    19681 tgattaattt tctctttatc atcattttgc ttcattaaag caacttcatc atttaatgct
    19741 ttaaatttag aatttaattt atcataatcc ttttttatat gtttgtagtc ttttactata
    19801 agttcatgtt ctatttttag agattcatat ttattcttaa aattactttg atttacctta
    19861 actaattctt cgtccaaatt actattaatt atattattgt tttctttatc tccatttatt
    19921 gcaacactgt tgtaaacatg atttttttcc aagtctttaa gtatatattt tgctctttct
    19981 atgacttcgt ctggtaattt agctaattta gcaacatata ttccataact cttgtctgct
    20041 ccctgaggaa ttatttttct taaaaatatt attccttccc catcttcttt gactgctatt
    20101 gaataatttt taacctcttt aaactcttct tctaaatcag taagttcatg ataatgtgtt
    20161 gcaaataatg tcttacatct aatatttttt tgaatatatt ccactattga ccatgctaaa
    20221 cttatcccat cataggtact agttccacga cctatttcat ctaatataac taaacttctc
    20281 tctgttgcat tttttaatat aagggatact tcattcatct ctaccataaa agtagattga
    20341 ccttgagata aatcgtcact agctccaact cttgtaaata ttctatctaa tattggaata
    20401 tcagcagact ctgctggtac aaatgagcct atatgtgcca taagagctat tatagcagtt
    20461 tgtctcatat atgttgattt acctgacata ttaggaccag ttattatgtt aataatgttt
    20521 tctccacgat ttaaataagt atcatttgga acgaaattct cctctcctac tatattttcg
    20581 acaacaggat gtctaccatt tcttatatca agtttattat tttcatttat tgctggtttt
    20641 acatagttat ttatatgtgc aactgttgca agggacacaa aaacatctat attagctata
    20701 gtcttagcca ctttttgtat tctatctatg tttttatata ttgtatctct tatctctaca
    20761 aatatctcgt attctaaaga tttaatcttt tcctctgcat gtagtatctt ttcttctatt
    20821 tcctttaatt ctggagttat atatctttct gcattactaa gtgtttgctt tcttatatat
    20881 gtctcatcta gctttgcctg tttaaagttg gcttttgtta tttctatata atatccaaaa
    20941 actttattaa atcctatttt taatgattta actcctgttt tttctctttc cctgttttct
    21001 atttccttta caagaaaggc tccattctta gaaatctctc ttagttcctt aagttcatca
    21061 ctaaaatctg atttaattat gtttccatct tttatggtta tcgtaggttc ttctagaata
    21121 gcttcatcta ttaaattgta tatatcatct aatttatcca tttctgaaac atactctttt
    21181 aggatttttg catttgataa atttatagta tcttttaaat taggtaactt ttctatcgaa
    21241 ttttttaagt gaattaactc ttttggtgtt actctctcaa atgctatctt tccacatatt
    21301 ctttctatat cgtatatatt tttaagtata tcatttaggt cttctcttaa tataaagtca
    21361 tcttttattt cttctattac atctaatcta ttttcaattt tagatttatt tatcaaaggt
    21421 tcttctacat attttcttaa aagtctacct cccattgctg tagaagtttt atcaagtaca
    21481 tgtaataatg aacctttttt cttattccct cttatagttt gagtaagttc taaattagtt
    21541 ctagtaaaca tatctaaaac catatattct gatgaattgt atatatttat attattaatg
    21601 ttagatgtta tttgtttttg agtattgtat atataattca gaagaattga taagcttgac
    21661 tttattagac ctttatcatc aaactttagt ttttgtagat aaacatctgg aaaatattct
    21721 tttaatatat ttatatccaa atagttatca ctaaaacttt catttatgta tatattactt
    21781 acagtagcta tatctctaag cttttctata aagtctaaat catttataat tatttcagtt
    21841 ggatggattt ttgcaatttc ttcaattacc ttatcttcat ttaaacatgt tgcattagtt
    21901 tctcctgtac ttatatcaac ataagttaga cctatattag taccatcctt atacaaagat
    21961 aataaataat tattcttttt attttcaagt aaatttccat ctaaaactgt accaggagtg
    22021 atgactctta taacctctct tctaacaatt ccttttgctg tagatgggtc ttccatttgt
    22081 tcacctattg ctactttata tccattttca actaattttg atatatatga attggctgag
    22141 tggaaaggaa caccacacat aggagcacgt tcttctaaac cacaagcttt tcctgtcaat
    22201 gctatttcaa gagcttttga agctactaat gcatcttcaa aaaacatttc ataaaaatct
    22261 ccaagtctaa aaaacagtat acagtcttta tatctatttt ttacttcaag atactgtttc
    22321 atcataggag ttaatttatt catatctatt tgcattatca aacctccttc ttttctaatt
    22381 aaatattata tcatagtaga tgattattat taacacaata tataaaattt tgacatataa
    22441 ctaaaaacaa tccaaaatgt atactcacat tttggattgc taagtttaat gcttattaaa
    22501 taaaaatata tatattttta caataaagat attatttctt ttttaagttt aatgatattt
    22561 tcaaggttat cttcatttaa ctttctacta tctataacaa attctttctt tactattgat
    22621 ggtttttttg aaataacata aattctgttt gatagcatta tagcttcgtc aatatcatga
    22681 gttataagaa gtattgttga atttactttt ttcttaacat ctaaaagcca tttttgcatg
    22741 gatgttttag ttaatgaatc aagtgcccca aaaggctcat ctaaaagcat tatgtcatta
    22801 gaatttataa aagttctcaa gaagttagct ctctgtctca ttcctccaga aagttctgaa
    22861 ggatatttat cttcatatcc gcttaatcca aacacctcaa aataaggttt taccattgac
    22921 ctagctttac tctttttttc tttttttaaa attagtggta gtgacacatt atctattata
    22981 gttttatatg gcaagagtaa atctttttga tacatataac tatatttacc atttatattc
    23041 actttgccac tatcataatc tgtaagttta gttataatat taaaaatagt actttttcca
    23101 catccacttg gccctaatat agaaacaaac tcaccttcat aaacatcaat acttatatct
    23161 tttaaaactt caatatcttt ataagttttt gatacatttt ctatagatac ttttactttt
    23221 ttattgtggt aaaaattcat ttgtgtaagc atcacttgcc ttcatatcct tatctattaa
    23281 tttatattct tttaagaatg atgtatagtt gttccataca ctatctttca tttctcccca
    23341 tcgtggtgca tcatctttgt acttgcttgc taaatattct tgactcttta aagctagttt
    23401 ttcatctact tcaggtgcat gttttactaa aatttttgct gattcttctg gattttttat
    23461 tgcatattca taaccttttg ttgtagcttt taagaatttt ttagcaagct ctggattatc
    23521 tttaagcaca gtttcattag aaattataag tggtgtataa taatccaatc tctcatctaa
    23581 atcttttact ggtataaagt ttaagtcata tccaattaaa tcagctttaa ctgcatccca
    23641 accttcaaat atccatgcaa aatctacagt tttcatagct gcaaagaaat catcttgacc
    23701 agtattaact atctttaact tattaaaatc agctccattt ttcttcataa ctgctttaaa
    23761 aacagcttct tctgatggag agccccaacc accataagtt tttccttcaa aatctttagc
    23821 tgttgttata ttcttttctt ttggtgatgc aaacccagaa gtattatgtt gtataacagt
    23881 tgctacagct tttattggaa gtggatcttc actagtcttt gcataagtaa cttgttcttg
    23941 atagcttata ccaaagtcag cttttcctgt tgctactaaa gtttctgctc cactttcagg
    24001 tggttgtact atttcaacat ccaaaccttc ttctttatag taacccttat caagtgctac
    24061 aaataatcca gtatgatttg tatttggtgt ccaatcaagc accatagtta cttttttaga
    24121 agaagtatct ttatcattgt ttttgtcttt attgttattt gaagaacatc cagttattgt
    24181 tgccaatgta aaaacactta ctaaacctaa tattcctaac tttttaaaat tcataattgc
    24241 ctccctcaaa tacctttaat caattaatct atgtcttata acaacttttt ttacagcctg
    24301 tacaattcct acaaacatta aactaaatat aactactaat atagtagatg caaatacttt
    24361 atcaagtgca tatgcacttt ttgacctaag catataaaca ccaagtcctt tcgttccacc
    24421 tagccattct gcaacagttg ctgccattac agcatacgtt gcagatattt taagtccaga
    24481 aaaaaattta tccattgcca taggaaattt aaggtgaatg aatgtattaa ctttatttga
    24541 gtccatagtc ttaaataaat ttaaataatc tttatcaata ttctcaatgc catctacaaa
    24601 acttattaat ataggaaaaa aacaagtaag cattaccatc aatacttttg gcattgttcc
    24661 aaatccaaac catatcataa aaagtggtgc tataattatt gtaggtatca tctgtgtaac
    24721 caacataata ggataaagac atttttttat tattgataca aaatccatca atattccaat
    24781 taccaatgat attataattg cgactataaa accaagtatt gcttctccta tagtaaccac
    24841 agcatgcatg gataaatttt gataatcttt aaaaaacaca ttaattatat ctactggtgt
    24901 gggtagtata tactgaggaa cttctaaaac tcctaccata gattgccaaa gaataagtat
    24961 aaacaaaaat gttgctattg gatatatact atctttgact ttatctatga tttctaagtt
    25021 tttcatctat agtcagacct ccagcttttt catctactct agctactgtt atgactcttt
    25081 cacatccaag attaaaaggg attttatgta ccttttttaa taattcaaaa acttcatcaa
    25141 aatcaccttc aacagtagtg ctcattgctc ctatttcaca tttaagacca cttttttcaa
    25201 tttcagcaat acaagcatct actactttat acatttcttc ttcacctttg taaggtctta
    25261 gtggcattac tgctatgtct gcgattacca tttaaatcac tcctttaaaa ataattaata
    25321 cctactaggt atattttgtt ttttattaaa aaattttatt actaaaaaga tattatattt
    25381 aaataaaaga aaagtcttat ccaaaatagg ataagactta tttttatctt aactggtctc
    25441 cctacactgg cattatccaa acaggttcaa agggtcgaga taaaatctcc tctcagcaca
    25501 ttagctcccc gaaatatatt atatcttttc tacttgatta tactacactt atcatatttt
    25561 aacaaatttt ttatttatat tagatatttt atccgttcaa aaatatctaa tatacattgt
    25621 acattattac accaaataaa ttatcttaaa atggtaattt atcaacatct tccaaaacta
    25681 gattaactga attttctagt ttttattttg ctatttcaag catatttgga tgtatatcaa
    25741 ttccatacaa atattgttct aaatttctgt tttcactaat ctaatttgca atcgttacag
    25801 attacagatt agaaatagct tttagactct acttatcttt atctacaaaa aatccttcaa
    25861 taaattcagt aataatattc agtaatctaa aaataaacct ctatctggat ttttaagata
    25921 caagcctttt aggaagtatc ttttgtgata atataaaaat tactcgtcat tatcatcaaa
    25981 atcctcagca accaacattt tagataagaa tcaaactcta taatataatt attttatcca
    26041 atttattttt gagaaataaa atattttttt aatttttaaa ttttttaata tgcaccttga
    26101 aaaaagaaac gttttcctat attatggtaa tagtagaata aaactaaata ggcaaaacta
    26161 gagaaattta gtgacgcaaa gctataggga ctaagactta tgaaatgagt tatgtcagcc
    26221 agttgccaaa gagatatttt ctttttgttt ttatgaatgt tttttagggg gatatatgat
    26281 atatgtttat ggataaaatg gatagatgta cacatatact aactgcatat atatgcagtt
    26341 cacgcgatta ctgcaatttt atagatacac aattaaatga ttttatacta gaatatggag
    26401 agaatgtggt agaatcttgt ttgcatcaag tgatggtatt ggtaagcaga tataattaaa
    26461 gaaattttat tttctagaaa acccttttta gttttatcta cgttttataa ttcattaaag
    26521 aactaatcaa aataagcttc attatatatt tttcatgaag taaacagttg agtttatctc
    26581 atatactgtt taccccaatt acataattct aataatatag attccaatga tttaccacgt
    26641 ttagtagaca aatattcaac ttttggagga atagtcggat aagaaattct cttaataagt
    26701 ccatctcttt caagatactt taattcttgt gacagtatct tatgacttat atgaacaaca
    26761 tcttttttta attgactgta acgtattggc tcttgtttta aaatatgcca taaaataatt
    26821 gccctccatt tacttcctat cacagtcatg gtaaaaccaa ttgaacaatt aaaatctttt
    26881 tgtctttctt caatatttgg aaattcttca aaattattat ctatatccat ctcaatcctc
    26941 tctttcaaaa tagtaagtat ctaactaaaa agtgcattct tgtttagcgt actaattcgt
    27001 aatataatct tgattaaaaa atagctacgc acactcgtga ctttagtcat gagttagtag
    27061 ctaaaatagt caacatatag ggaaacctat atgtagagat aggatagaac ctatccaata
    27121 ctacttgaat tgctaaaaac ccctaaagct aaccaaacta aaacataagg atgaaataaa
    27181 cctaagtgtg aaagttacga aagtagaaaa aattggttag atggtataag gttaaaccct
    27241 aagtacttta aaatgggtaa tcagcaggta aactccgaat aggagaaact tcaacgacta
    27301 ttcctcttga gggaagtaca ctacaagtta tcggtagtgg aagtgggtag accttaacgg
    27361 ataatgccga aggataagat atagtctgtg cttatgtgaa agtgtaagaa gttcataaga
    27421 gaactgcata ggtagtagcg aa
//
LOCUS       DAELSY010000038        27439 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.038, whole genome shotgun sequence.
ACCESSION   DAELSY010000038 DAELSY010000000
VERSION     DAELSY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27439
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.038"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            435..3983
                     /locus_tag="KPG09_001471"
     CDS             435..3983
                     /locus_tag="KPG09_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0966090.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINNEMVIALMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTISCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKIIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDNFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADMALY
                     SAKGKGKNCYTLYDKKIDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESASNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMRNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEVTSIEAEENT"
     gene            4168..6102
                     /locus_tag="KPG09_001472"
     CDS             4168..6102
                     /locus_tag="KPG09_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBG0966091.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLENAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            6208..7203
                     /locus_tag="KPG09_001473"
     CDS             6208..7203
                     /locus_tag="KPG09_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0966092.1"
                     /translation="MKKYISIILLVVVTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSNIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            7175..7960
                     /locus_tag="KPG09_001474"
     CDS             7175..7960
                     /locus_tag="KPG09_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG0966093.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7948..8646
                     /locus_tag="KPG09_001475"
     CDS             7948..8646
                     /locus_tag="KPG09_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0966094.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8769..9242
                     /gene="ybaK"
                     /locus_tag="KPG09_001476"
     CDS             8769..9242
                     /gene="ybaK"
                     /locus_tag="KPG09_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBG0966095.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9439..10278
                     /locus_tag="KPG09_001477"
     CDS             9439..10278
                     /locus_tag="KPG09_001477"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBG0966096.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10291..10839
                     /locus_tag="KPG09_001478"
     CDS             10291..10839
                     /locus_tag="KPG09_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBG0966097.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10905..12086
                     /locus_tag="KPG09_001479"
     CDS             10905..12086
                     /locus_tag="KPG09_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBG0966098.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYIYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12259..12522
                     /locus_tag="KPG09_001480"
     CDS             12259..12522
                     /locus_tag="KPG09_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0966099.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            13048..13731
                     /locus_tag="KPG09_001481"
     CDS             13048..13731
                     /locus_tag="KPG09_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0966100.1"
                     /translation="MSSILLVEDDLSLIYGLEFSIQKSGFSIDIARTVKEAMQMYEEK
                     NYDLLLLDVSLPDGNGFQICEKVREVSNIPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNVASNVNELSSNNITIKLLENRVFKGEFEVELTTAEYKLLC
                     LFMKNKNIVLTRKNILDKLWDGNGSFIDDNTLSVYVRRLRNKIEDNPENPKFLLTVRR
                     MGYKWNVIK"
     gene            13742..14983
                     /locus_tag="KPG09_001482"
     CDS             13742..14983
                     /locus_tag="KPG09_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0966101.1"
                     /translation="MKIFSNKDIKIFFLILISILVMFIIVGQIVVISITNNYKKTLIE
                     HDYNIAGYLYSIGVDKSKIPSAFVAHEDKYILKGKEILDTAGYGFDIDVSFTPSVKLF
                     QSKYIKTMSIYLLVFFTMIIFILYMYFFRQQSKIEEASYKIDLFMNGNINERLNTYEE
                     GSLSKLFSSIDVMSTSLNTHILKEKQSREFLKNTISDISHQLKTPLTALIMYNQILQE
                     ESHNSDVVEAFVQKSQSELERIETLIKNLLKITKIDSKTIVLNKNNVNLKNLIVKILY
                     SFETRAEKEGKVISINGSENTLLFCDYEWMSEALSNLIKNALDNTKENDKITIEWFET
                     TITTIVSVKDTGNGIHVEDIHHIFKRFYRSKFSKDNQGLGLGLPLVKSIVEQHNGTIS
                     VESKLSQGSTFTISFLKLTNM"
     gene            15055..15738
                     /locus_tag="KPG09_001483"
     CDS             15055..15738
                     /locus_tag="KPG09_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0966102.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGRIFIDGKEICSMKEEKQTIFRRRNIGFIFQAYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKNHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSNDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGV
                     LTDLGVNRE"
     gene            15735..18065
                     /locus_tag="KPG09_001484"
     CDS             15735..18065
                     /locus_tag="KPG09_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0966103.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIIIAVCLVTAIFGLADMAI
                     QCQKIQFIKDDGNWHIVIRNIDEKFISEINSRVDIAHHGRGQVVDSGEFKSKEILIQG
                     LDEEVAKQFNLEIIRGRYPIGENETLIDEDAVKSFNLKIGDFIKIKLADGRNKNYKIV
                     GEYSSISSLRATDKHGLILSILGTQAIKKSETYDYYYITFKEKVNIRKATSDIKNTFN
                     LSDKQIGKNERLLSIIGQGDSDFSKPLYLTAGLLFVLVLVASIIMIYNSFNMSVIERV
                     KFFGLLRCLGSSKHQVKKFVVLESLILSIKAIPIGLVLGSLVTIISSIFLKYVNELFS
                     SMPILKVSFIGIAFGIIVGFLTVILSAIIPAKKASRVSPLSAVRGNLMSKEILNNGKT
                     SKVANTGSFKIETSMGLNHAFSRKKSFVLMTSSFAISIILFLGFSVLIDLMYQGLKPM
                     KPEAPHISIVDPNFKSVLTKEDINEISNIKGVKKVYSRMMTDIEATYNGKKTKSQLIS
                     YEKYQFNWAKKYLLKGEVNENKLQSENLALVEEGHGFDIGDKIYIKNRNAEKELEVGG
                     ILSSIPFEAKDKYIGNIITSENTFTNITGIKGYIIMDIQVDKDTPGDLIENIRRVVDD
                     NLEVRDKRQSNLEVKNSFYTMATFVYGFVFIIAIISMFNIINSMNISVTSRKNYYGVM
                     RAVGISNRQLRRMVIVEAGSYAMSGCLLGSILGLMLHRYMFISLVSSMFHLEWQVPFN
                     LLLITVLTMIIITLLSVRKPVKKICELDIIDSINAQ"
     gene            complement(18246..19088)
                     /locus_tag="KPG09_001485"
     CDS             complement(18246..19088)
                     /locus_tag="KPG09_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG0966104.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKAGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            19383..20117
                     /locus_tag="KPG09_001486"
     CDS             19383..20117
                     /locus_tag="KPG09_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0966105.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            20127..21260
                     /gene="nagA"
                     /locus_tag="KPG09_001487"
     CDS             20127..21260
                     /gene="nagA"
                     /locus_tag="KPG09_001487"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG0966106.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNITHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            21359..22108
                     /gene="nagB"
                     /locus_tag="KPG09_001488"
     CDS             21359..22108
                     /gene="nagB"
                     /locus_tag="KPG09_001488"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBG0966107.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKLENTHLP
                     NGMADDIEKECLNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            22298..22555
                     /locus_tag="KPG09_001489"
     CDS             22298..22555
                     /locus_tag="KPG09_001489"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966108.1"
                     /translation="MFSGILSSIIKLPIIGILPIGNIKKNTGISDSKKDGKQRRSNLG
                     ISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            23215..23919
                     /locus_tag="KPG09_001490"
     CDS             23215..23919
                     /locus_tag="KPG09_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0966109.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILDTNI
                     LIETVWGFGFRLKEKS"
     gene            23921..25408
                     /locus_tag="KPG09_001491"
     CDS             23921..25408
                     /locus_tag="KPG09_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0966110.1"
                     /translation="MNNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVFSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            25613..27226
                     /locus_tag="KPG09_001492"
     CDS             25613..27226
                     /locus_tag="KPG09_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0966111.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYEILEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNIHSQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQASFGNKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDSSVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
BASE COUNT        10948 a         2785 c         4757 g         8949 t
ORIGIN      
        1 aacttaagtt ttgagtatta atctaaaata tatgttttta tagggtttaa attttatgta
       61 tacaaaatta atttgctaac tgaatcatat aaaaaaaaca aaatattttt tagagggaat
      121 gattagatac tattttcttt gttatgaaat taattgaaga gtaattaatt agaaaaggaa
      181 taaataataa tgaatatatt acattattac tttttgataa atcaaaaata aagttatagt
      241 ttttaatatt aattaagaat gatgaaatat gaataaagat tttacaaata aatcttaagc
      301 tatcttattt aattctaatg caggaagaag gtaaatgtat attgaaaaaa tcatagaaat
      361 agaagtaaac aacttaaaca aatacaaagg tttgcaaaag aagtaatata attatataca
      421 atgaggatgt gagtatgatg aacagactaa agccaagaat tttaggtatt agtatcttgt
      481 ttattattat aattatttta acatgtggag ctgttttcaa gtttaaattt aaattagata
      541 ataaattaaa agaaaccaca catacatatt tgtctgagtt tacagaacac aatataatta
      601 atctcaagac aagacttagt ggtcaatttg acatgcttga atctattgct aacttcatag
      661 gaaacttgaa taatataaat aatgaaatgg tgattgcttt aatggattct gatgtaaagc
      721 gaagttcttt aattagaatg actatctcta cattagatgg ggaatgttat tctaatgatg
      781 gaatcaccca taatgttcaa gatagagatt attttaaaaa agctaaaaat ggagaaaaga
      841 atatttcaga gcctttaaaa tctattgtta ataataaaga aataatagtt ttatctaccc
      901 cagtttataa aagtgggaaa atagttggtg ttttaagtgg aatttatgat tcaaaaaagt
      961 taaatgagtt attaggatta tctgcatttg atgaaaaagc agatgttttt gttgcaaaga
     1021 aaaatggaga tatagttgct caaaaaacaa ataaaaatag aaagacgata tcatgtatat
     1081 ttcagctttt tgacaaagat aattgtgatg aagatgattg tctaaaaact ataaaagaaa
     1141 atatgaaaaa tgataagtct ggatatttta attataactt agataatgaa aaatttatgt
     1201 taaactatca gccacttgga gttaatgatt ggtatgtttt ttctgttgtg ccaaataaaa
     1261 tagtgtctat tcagtcagag aatatatctt atgatgtata tatttttgca ttagaaatac
     1321 tactagttat attgattttt ataatatata tagcatgtat tataaataat aacaccaaaa
     1381 ttattataaa tgaaaaagaa aaatttaaag tactcactga taatatatta ggaggggtta
     1441 aaacctgttt aaaagatgaa tatatgacta tgacatatgt aagtcagggg tttttagagc
     1501 ttacaggtta ctcttgtgaa gatttaaata aaatatttaa aaatagattt aaagacatga
     1561 tttataaaga tgatttagac aagtttaatt gtattattct aaatcaatta gaaaaacaag
     1621 ataaaattga agttgaatat agaattgtaa gaaaagatgg aagtataatt tggattttgg
     1681 agagaagtaa gtctataaat gataataaag gaagtgagtg tattcaggga gttttaactg
     1741 atataacaga acttaagaaa atacaagaag agttagattc aaaaagattt gaaatacagg
     1801 ctatcaatga tagtattgtt ggtggaatag tgataactga gataaatgat gattttgata
     1861 gtatatattt aaatgaaggt tatttaaata tgattggata tacacgcaaa caactggaag
     1921 atgagctaaa taataaatta atcaaactta tttttaaaga tgatagaaca gaattaatta
     1981 atagtataaa caaacaactc aaattaagtg atacatttgc atctgaacaa agagttgaaa
     2041 aaagggatgg tactattata tggattttat taaaaggaag aaagataatt tatgataatg
     2101 gaaaatctgt actttgttgt gttattgtag atatcacttc tgctaaatta ttagagcaag
     2161 aacttaaaaa gagtgaagaa cgtcatgaaa tcattctaaa gcagacgcaa aatatcataa
     2221 tagaatggga tatagaaaaa gataaaatga atatatcacc taattggtat aataagttta
     2281 aatatgaccc tataactgaa aacgcaacaa aaaatataat taaatcagta gatatatatc
     2341 atgatgatat ggataagttt atttcattaa ttaaacttac ttcacaaggt agtgagtatg
     2401 aagagtgtga aattagactt agaactagag atactattta cgcttggttt agtataaagg
     2461 caacaacttt atttgataat aatggtaaac cttataaagt agtaggaagt ttgactgata
     2521 ttgatgaatc aaaacgagaa gctgaaaagt tgaaagaaag ggcagaaaaa gaccctctta
     2581 cagatttgta taataaaaaa gcatcacaat cacttataga agagtacata acattggata
     2641 aaaaaagaaa aggtgcttta atgattattg atattgataa ctttaaagga ataaatgata
     2701 atttaggaca tttatatggg gatgctgtat taagtgaaat tgcaaatgac ctaataaatc
     2761 tatttagagc aaccgatata gtaggcagaa ttggtggaga tgagtttatt gtatttataa
     2821 aagatatttc tgaaacaaga gatattataa ataaagcaga agaattggta aaaacatttg
     2881 aaaggtcttt tttaggtaaa ggacgaaatt ataaaatatc attaagtgta ggaattgcta
     2941 agtttccaca agatggtatg gactatatga cactatttag aaatgcggat atggctcttt
     3001 actcagccaa agggaagggt aagaattgtt atacattgta tgataaaaaa attgataaag
     3061 tccaatatat aactaataca agaaatgcag gagatagtga taatgtagag atgtctttta
     3121 aagaacatat cacaaattat atatttgata tactatatga atcaagtgat atagaagtgg
     3181 cagtaaattt aattctaagt attgtaggta aacactttaa tgtgagtcgc atgtatatat
     3241 ttgaaaattc agatgataat cgagtatgta aaaatacatt tgaatggtgt aacgaaggtg
     3301 taacctcgga aattaataat cttaaaaata tttgttacaa ggatttaggc aactatcttt
     3361 taaattttaa tgcagatggg ttattttatt gtaatgatat tgaaaattta ggtagtgatg
     3421 tgaaaaaagt acttaagcca caaggcatac gttcaatatt acaatgtaaa attttagagg
     3481 gcaatgaatt tagagggttc ataggatttg atgaatgttc atataatcgt atgtggacta
     3541 atgatgaagt gaacactttg acatttatat ctaaaacgct cagtatgttt ttattaaaaa
     3601 tgcgtattca gagtaagcta gaagagtctt taacaattca aaaatctgtg atggataata
     3661 tggatatatg ggcatatgtt gttgacagga atacctatga attattattt atcaataaaa
     3721 aaatgcttga gtcagcatca aacactcatg taggtgattt atgttataaa gcgatatgga
     3781 ataatcaaga taaaccttgt aaaaaatgcc ctatgatata tttaaaagat ggtgatgata
     3841 cttataaatc atttatgcgc aataaaaagt ttgaggtaca ggcaaatata actacaacta
     3901 ctttgaaatg gtttgatggg aaagatgcat gtttaatatt tggaagtgaa gtaactagta
     3961 ttgaagctga agaaaatact taaaatactg tgttatatat atacttaaaa tcgcaatttg
     4021 aatttattag acttaaaatg tctatataaa tcatttaagt tgtggtttta agtatttttt
     4081 atgatataat taagcgttaa gaacaaaatt aaaagatact tttgtataaa aaaatatttg
     4141 ttaaatatat attgagcagg tgataatatg gaaaataagt ttttaccaat aagcaaacaa
     4201 gatatgatag atagaggatg ggaagaatta gattttgttc tagttacagg agatgcttat
     4261 gtggaccatc atagttttgg aacagcaata atatctagag ttttagaaaa tgctgggtat
     4321 aaagttggaa taatagctca accagactgg agaactacag atgattttat gaagctagga
     4381 aaaccgagac ttgcatttct agttaatgca ggaaatatgg attcaatggt caatcattat
     4441 tctgtaagta aaaagcatag agaaaaggat atgtattctc caggtgggaa aatgggttat
     4501 agaccagata gagctactat agtatattgc aataaaataa gagaagcata cagtgatgtt
     4561 gcaatagtaa taggtggaat tgaagcgagt ttaagaagat ttgcacacta tgattattgg
     4621 agtgataagg ttagaaagtc tatgttaata gatagtggag cagatttatt agtttatggt
     4681 atgagtgaaa agcagattgt agaagtagcc aatgcactta atgatggata tgacccaaaa
     4741 tatattagac atattgatgg gacatgctat atttcagata cattggaaga aatatatgat
     4801 aaatatatat taattccatc atataaagaa atttgtgaag ataaaatgaa gtatgtagaa
     4861 gcttttaaaa ttcaatatga tgagcaagac ccatttagag gtaatataat tgttcaacct
     4921 catggaagta agtatctggt tcaaaataag ccagaaaaac cacttagtag agaagaacta
     4981 gatgaagtat atggattacc atatcagaag acataccatc ctgtgtatga aaaatttgga
     5041 ggaataccag ctatagaaga agtaaaattt agtatagtaa gttcaagagg ctgttttggt
     5101 agttgttcat tttgtgcaat aacatttcat caaggtagag cagtgcaaag tagaagtgaa
     5161 aaatcaatta ttgatgaagc aataggaata acaaatttag atgattttaa agggtatata
     5221 catgatgttg gaggtccaac agctaatttt agaagaccag catgtaaaaa acaaattact
     5281 aaaggtgctt gtaaaaatag acaatgctta tctccatcac catgtaaaaa tttagatgca
     5341 gaccacagtg aatacttaca tttacttaga gctgtaagaa aattacctaa aataaaaaag
     5401 gtgtttgtgc gttctggaat tagatacgat tatgtaatgg cagataagaa caataaattt
     5461 cttagagagt tgatagagca tcatgtaagt ggtcagttaa aggtagcccc agaacatata
     5521 tcagaagaag ttttaaagta tatgcaaaaa ccagcaggaa aaacttatga taaatttaga
     5581 cagaagtttt ttgctataaa tgaagaatta ggtaaaaagc aatatttgat accctattta
     5641 atgtctagtc accctggttc taccttaaat agcgctatag agttagcaga gtatttaaga
     5701 gatactcact accaaccaga gcaggttcag gatttttatc caactccagg gactctatct
     5761 acaaccatgt tttatacagg tattgacccg cttacaatga agccagttta tgttcctaaa
     5821 tcaaaacgtg acaaggcaat gcaaagggct ttattacaat atagagcacc tagaaattat
     5881 gatttggtat actctgcgtt agttgaagca ggaagagaag acttaattgg ttttggacat
     5941 agatgtctta taaagccaaa aaatgaaaag ccttatttta atagaaataa ttctaaaaaa
     6001 aatgtatcta aaggaacaaa taaaaacaaa aaaactaata caaataacag aagtaataaa
     6061 aatcaacaaa aaagttcaac taaaaaaaag aaaaaacatt aaagtataaa taaaaatact
     6121 taaaaaagaa ggattattcg ataaattaac agaaattagt ataaataaat ataattattt
     6181 taatgacgtt acctttggga ggaagagatg aaaaagtata tttctataat attattagtg
     6241 gtggtgacta tggttgtttt ggtaggatgt tcaccaggaa aagataaccc taaaaataag
     6301 gagctatcag ttgtcaaaca agtaaaagta gctgttccag atggactacc agcagtggca
     6361 atagctaaac ttgcaaatga aaatccagag attaaagaag gatatgaaac agtgtatagc
     6421 attgaaaaga cacctgaggc gatttccact agagtgatga aaaaagatgc agatatagct
     6481 atagttcctt caaacatggc tgcaatagcg tataataaaa cttcatcata taatattgtt
     6541 ggaactgtag gaatgggttc actttatttg gtgtcaacag aaaatataaa agattattct
     6601 gatttagttg gtaaagaagt tggatgtact ggaaaaggac ttactccaga cataactata
     6661 aaaagtcttt tgttacaaaa ggatataaat tattcaaata taaaatttaa ttatgtaaat
     6721 tcagcaagtg aactggtacc attattggct actggtaaag ttaagacagg tattgttcca
     6781 gaacctgcac ttagtgcttt gatgtctaaa aatccagaca taaaaattat aaaaagttta
     6841 aatgactcat ggaaggaagt aagtggttca aaagacggtt atcctcaatc tactttaatt
     6901 gtaaagtctg aatttttaag agataataaa gattttgttg attcttttgt aggtcaatta
     6961 tcaaatagta tagattgggc aaacaaaaat cctgaagaat taggtgcata ttctgaaaaa
     7021 ataaaaatat ctactgaatc aaagataata ggaaaatctc ttgaaagagc taatttaaaa
     7081 tatattccag ttaaggacat gattaaagat tataaaaatt actatgagaa attagctaat
     7141 tttgatgata agacattagg aggcaaagta ccagatgaag caatatactt tgttgagaaa
     7201 taaaaaaaat aaagaacaat ttatagataa aatggaggta acactttctt gtgttatcct
     7261 cttattttta tggcaaatta ttgctttaaa aattgataat gatatttttt taccaacagt
     7321 aggtcaagta tttaatagta taaaagagat tatgctaaat tctagtttct atatagatat
     7381 actttactct atggggagat gtttttttag ttttttactg gctatgattt ttgccattat
     7441 actttcaata gtatcttatt taaatagatt ttttagaaac ttattaaagc caatcaatgc
     7501 tttgacacgt tcaataccaa ctatgatatt ggtagtactt gcacttattt ggtttgaaaa
     7561 ggatagtacc ccttttatag ttggatttac tatagttttt cctatattgt atgataacgt
     7621 tttgggcgct atattgaata tagacaagaa cttattagaa atggctaatg tgtataaaat
     7681 aaggtttata gataaggtgt taaaaattta tttaccagcc ataaaatttc aaattatatc
     7741 tatactagta tcaacattct ctttaagttt gaaagttgct atagcaggag aggtatatag
     7801 tcaacctaca tatggtattg gaactatgat acagactgaa aaaattaact ttaatacatc
     7861 tggaatattt gcatggataa ttattgttct tttaatatct gctattttac aaatagccca
     7921 aaagttttta gcaaggagag cttttttatg gaaaagataa agttgagtat tgaaaatata
     7981 aataaaagat atgatagtcg tattatattt agagatttta atattgattt ttatgtaaat
     8041 gaggtaaatt gtatccttgg aaaatctggt tgtggaaaaa ctacactctt gaacataata
     8101 agtgggatta ttaaaaatga caccaacaat ctaaatatca aagaaaattt aaatagagta
     8161 ggaaataagc ttgaagctag ttacatattt caagatgata gacttataga ctggttaaca
     8221 gtagaagaaa atataaagat agttgtaaat aaatactata ataaaactca attaaataaa
     8281 atttgtgatg aatacttgga attggttgga attagtgatt ataaaaaatt ctatcctcag
     8341 atgcttagtg gaggtattag acaaagagta aatattgcta gagcttttat atatccatca
     8401 aaaaacatta taatggatga accttttaaa tctattgatg caaagaatac acagcttata
     8461 atggataatt tcagaaatat attaagaaaa gaaaagagaa ctgtattatt tgtaactcat
     8521 aatattgagg aagcattgtt tttagcagat aggatattta ttttaggaga ctctccaatt
     8581 agaataaaaa aaatattaaa aaattctaag gaattggaaa aaaatgaagt tttgaagttg
     8641 atatagagtt ataataatac tgtcgtttta aaatatattt ggttaaaatc tataagacta
     8701 tattaaaata gtacagaata ttcaaaactg tgtataatgt tcgtatttta taaaaaggaa
     8761 gtgaatgtat ggttaagact aatgctatga gaatattaga ttcaaataag attgattata
     8821 aagtaatgtc ttatgaagtg aaaagtgaac atgtggatgg tgttgaggtg gcacatgata
     8881 taggaagaga tgtaaatgaa gtgtataaga cattagtaac tcaaggagtt agtaagaata
     8941 tatatgtata tgtaatacca gtacatgaaa acttagactt aaaaaaggct gcaaaagtag
     9001 ctaaagagaa aagtgtagag atgattcatg taaaagatat aaataagctt acaggttata
     9061 ttagaggcgg atgttctcct ataggtatga aaaagcttta taaaactttt gtaaatgaaa
     9121 gtgcaaagaa tttagataca attattgtga gtgcaggaaa aataggatat caaatagaac
     9181 tatctccatt tgatttacaa agacttataa aagtagaatt tgtagatgta ataaaaaaat
     9241 aagaattaca tataaaatat agagcttata ttcttaataa taggtgttgt aatattaata
     9301 aaatgaataa taattttaaa ataaatattc agttacttat gtgtaattgg tataaaaata
     9361 ttgagaaatt ttaaaaacaa tggtaaagtg taagtataat cacgaatatt ttttataaag
     9421 taatggaggg atgttcgctt gagaaaaata aaatctgaac aaatcgtcga gcaggtaaaa
     9481 aaactttgta tagaagcgag cctatattta ggagaagatg ttttaagttg tataaaagaa
     9541 aaagcgaaaa gcgaaaagag tgaagttgga aaaaatatat tgaatatatt agttgaaaat
     9601 gctgaaatag ctaaagaaaa aaatatacca atatgccaag atactggtat ggctgtattt
     9661 tttgttgaaa taggacaaga agttttaatt gaaggagaca cacttactga tgctataaat
     9721 gagggagtaa gacaagggta tgaagaaggc tacttaagaa aatctgttgt aagtccaatt
     9781 aacagagtaa atacaaaaga taatacacct gctgttattc attatgatat ggtaaaaggt
     9841 gataaaatta aaatagaatt tgcagctaag ggatttggaa gcgaaaatat gagtaagatg
     9901 aaaatgttaa agccatcaga tggattggaa ggaattaaaa aattcataat agatacagtt
     9961 tcagaagctg gacctaaccc atgccctcct atggtaattg gagttggtat aggtgggact
    10021 gtggacaaat gtgctcaaat agctaaaaag gcacttttta gagaactagg tgaatttaat
    10081 aaggacgaaa atatagcaaa acttgaaagt gaactattaa cagctattaa taaacttggt
    10141 ataggacctc aaggcttagg tggtactaca acagctttgg gattaaacat agagactttt
    10201 cctacacaca tagcaggtct acctgttgtt gtaaatataa actgccatgc atcaagacat
    10261 aagaaggtag taatttagga ggaaaataaa atgataaaaa taactacacc tgtaaatgaa
    10321 attgatatag caaaattaaa ttgtggagat acaattagtt tgagtggtat actttataca
    10381 gcaagagatg ctgctcataa aagattgata gattgtataa ataaaggtga agaattacca
    10441 tttgacgtat atggtcaagg aatatattat gtaggaccta ctcctacaaa gccaggagaa
    10501 gttataggtg ctgcaggtcc aacgacgagt tatagaatgg atgatttgac tatacctctt
    10561 ttagaaagag gtcttagatt gatgataggc aagggaaaaa gaagtgatga agttatagaa
    10621 ggtatgcaaa aatacggagc agtttatctt gctgcaatag gaggagctgg agcgtatatt
    10681 tcaaattcaa taaaatcatg tgaaattatt gcatatgaag atttaggagc tgaagctata
    10741 agaaagattg tagtagaaga ccttaaactt acagtagcaa tagatagcta tggaaataat
    10801 atatatgagc aaggtcgagc aatttatgaa tgtaaataat gtttagaaat aaatatgaat
    10861 atcaaaatta aaaatagttg tgtatatttt aaggaggaaa attgatgtct aaggattatg
    10921 caaaattggc attagaaatg catgaaatca acaaagggaa agtaagtgta gaaagcaagg
    10981 tagaagttaa aactaaggat gatttatcaa cagcatatac accaggggtt gctgaacctt
    11041 gtttaaaaat acatgaaaat caagatgatg tttatatata cacttctaaa gggaatctag
    11101 tagcagtagt tagtgatgga agtgctgtac ttggattagg taacattggt gctgaagcat
    11161 ctatacctgt tatggaaggt aaagctatat tatttaaaca atttgcagat gtagacgctt
    11221 ttccaatatg tttaaagact aatgatgtag atgagatagt taaatctgta gagttgatgg
    11281 agcctgtatt tggaggtata aatttggaag acatatcttc tccaagatgt tttgagatag
    11341 aagagagatt aaaaaaatct ttaagtatac ctgtttttca tgatgaccaa catgggacag
    11401 caataatagt tgcagcagcc ataataaact caataaagtt aattgaaaat aaaaaaatag
    11461 aagatttaga aatagttata aacggagcag gagctgctgg tatagccata gcaaagatac
    11521 tacttaatat gaatgttaaa aatataatct tatgtgatag aagtggagca ttggaagcta
    11581 gtatagaaaa tctaaactat gttcaaaaag aaatgttaaa agtaacaaat ataagaaatg
    11641 aaaaaggacc tttaaaagat attataaaag gaaaagatgt atttattggt gtttcaggtc
    11701 caggagctgt tacaaaggaa atggttgaaa gtatgtctga gaagccaata atattggcaa
    11761 tggcaaatcc tactccagag ataatgccag aagaagcaaa attaggtgga gcatttataa
    11821 tgggaacagg tcgttcagac ttccctaatc aagtcaataa tgtactggct tttcctggaa
    11881 tatttagagg agcattagat gttaaggcta aagagataaa tgaagaaatg aagatagctg
    11941 ctgcatatgc aattgctaat acaataagtg atgaagaaat atgcccagag tatatattac
    12001 cagatgtatt taataagaat gtagtaaaaa atgttgctaa ggcagttaaa gaagcagcga
    12061 taaaaactaa agtaaataga atataaattt ttataactta ttaaaaacta gaaaagtata
    12121 aaagactagt gaaattatgt acagtgggat gattttatta gtctttttat tttaaatatg
    12181 acacaataat gtcatattaa gcttgatata attttaatat ggttaggagc caaagaaaaa
    12241 tataaggagt gatttaaaat ggaagaaact aaattttgcc aatgctgtgc aatgccaatg
    12301 ggacaaactg atgaattata tgggacaaat aaagatggaa gtaaaagtac tgattattgt
    12361 agttattgtt ttaaagatgg tgagtttaca gctgatataa gtatggaaga gatgatagaa
    12421 gtttgtatcc ctcatatgct tcaaagtaat aaggatatga ctgaagatga agcaagaaca
    12481 atgatgaaca atttctttcc aacattaaaa cgctggaaat aattttatgc aaaattagtt
    12541 atatgcaaaa catgaaatat ggtgttgaga ataaggggtg gtgtgcttta tagcatacca
    12601 cctgttttgt tacttgctag ttcagttatt cttgtatgct taaatatgtt ctcatctctc
    12661 tttctatttt aaattgtaaa aatctaaaat atgttattta tattctactt ctttatccat
    12721 gtctttttct acctcaagtc aaatacaact acaaaatcat cattttcaat tcactgttta
    12781 atagaattaa cagataaaga gttataaaat tttctatttt ttatataaaa taatacaggt
    12841 caagctgact tctagctact tgggatagtg tatatattta tgatagttat aggagtaata
    12901 ccaatcatta gactgttgat ttgtgtagtc ttttgtaaaa acatttgttt taatatattg
    12961 agtttttgaa aaagtaaaat tataattata ataaattaag tgtaataatg tattagaagt
    13021 aaaaataatt aaaatagagg tgattaaatg agcagtatat tgttagttga agatgattta
    13081 agcttgatat atggattaga gttctctata caaaaaagtg gatttagtat agacatagca
    13141 aggacagtaa aagaagctat gcaaatgtat gaggaaaaaa attatgactt attgctttta
    13201 gatgtatccc tacctgatgg taatggattt cagatttgcg aaaaagtacg agaggtttct
    13261 aatataccca ttatattttt gactgcttca gatgaagagg taaatgtagt gatgggtctt
    13321 gatatgggtg gcgacgatta tataacaaaa ccttttaaat taaatgaatt aatttcaaga
    13381 ataaaagcat tacttagacg ttataatgtt gcaagtaatg tgaatgaatt aagttcaaat
    13441 aatattacta ttaaattact tgaaaatcgt gtttttaaag gtgaatttga ggtggaactt
    13501 actacagcag aatataaatt attgtgctta tttatgaaaa ataaaaatat tgtacttact
    13561 agaaaaaaca tattggacaa gttatgggat ggaaatggta gctttataga tgacaataca
    13621 ttatcagtat atgtaagacg attgagaaat aaaattgaag ataatcctga aaatcctaag
    13681 tttctattga ccgttagaag aatgggttat aagtggaatg taattaagtg agggtattaa
    13741 tatgaagata ttttctaata aagatataaa aatatttttt ttaattctta tatctatttt
    13801 agtaatgttt attatagtag ggcaaatagt ggttataagt ataactaaca attataaaaa
    13861 aacgttgata gaacatgatt ataatatagc tggatactta tatagtatag gtgttgataa
    13921 atctaaaata ccatcagcat ttgttgcaca tgaagataaa tatattttaa aaggaaaaga
    13981 aatactagat acagctggat atggatttga tatagatgtt agctttacac caagtgtaaa
    14041 attgtttcaa agtaaatata taaaaactat gtctatctat ttattagtat tttttactat
    14101 gattattttc atactatata tgtatttttt taggcaacaa agtaagatag aagaggctag
    14161 ttataaaatt gatttattta tgaatggaaa tataaatgaa agactaaata catatgaaga
    14221 aggtagctta tcaaaattat ttagttcaat agatgttatg tcgacatctt taaatacaca
    14281 tatattaaaa gaaaaacaga gcagagaatt tcttaaaaat acaatttcgg atatatcgca
    14341 tcaattaaaa actccactta cagctttaat aatgtacaat cagatactac aagaagaaag
    14401 tcataatagt gatgtcgttg aagcttttgt acaaaaaagt caaagtgaac tagaacgcat
    14461 tgaaacattg attaagaatt tactaaagat tacaaagata gattcaaaaa caatagttct
    14521 caataaaaat aatgttaatc ttaaaaattt aattgtcaaa attttgtata gctttgaaac
    14581 tagggcagaa aaagaaggaa aggttatttc tataaatggt tcagaaaata cattgttgtt
    14641 ctgcgattat gaatggatgt cagaggcttt aagtaatctt ataaagaatg cattagataa
    14701 tacaaaagag aatgataaaa taacgataga gtggtttgag acgactataa ccaccattgt
    14761 ttcagttaaa gacactggaa atggaatcca tgttgaggat attcatcata tatttaaaag
    14821 gttttataga agtaagtttt caaaagataa tcaaggttta ggactgggat taccactagt
    14881 taaatctatt gtggaacaac ataatggtac tatttctgta gaaagtaaac tatcacaagg
    14941 aagtacattc accataagtt ttttaaagct tactaatatg taagataaaa tacatcttgc
    15001 agtaaggtat atagattatt attatattaa tcaattagaa aggtgagaga gaaaatggat
    15061 attcttgtgg ttaataattt atgtaaaaca tatggaaatg gatatacaaa agtagaggca
    15121 ttgaaagatg tatcctttac agtttcaaaa ggagagtttg tgactgtagt tggagaatct
    15181 ggttcaggaa agagtacact attaaatctc ttaggaggtc ttgatactcc aacatcaggg
    15241 agaatattta ttgatggtaa agaaatatgt tcaatgaaag aggaaaaaca gactattttt
    15301 agacgtcgta acatagggtt tatatttcag gcatataacc ttataccaga actaactgtg
    15361 gagcaaaata taatattccc attgttatta gattataaaa aaccagatac tatttactta
    15421 gatgaagtat tgactatttt aggtcttaaa gataggaaaa atcatcttcc aaatcaatta
    15481 tctggtggtc aacaacagag agttgcaatt ggaagggctt tgattacaaa gccaagtatt
    15541 atactggcag atgagccaac gggaaatctt gacagtaaaa atagtaatga tgtaatttca
    15601 cttttaaaag tgactgcaga aaagtataat caaaccatta ttatgataac tcataatacg
    15661 aatatagctt ctacagcaga tagaatacta caggtttctg atggtgtgct tacagacctg
    15721 ggggtgaata gagaatgaga agctacttag aattagtacc acaatatgca aaagtacata
    15781 agaaaaaaaa tagaactaca attttatgta ttattattgc agtatgctta gttactgcta
    15841 tctttggact tgcagatatg gctatacaat gtcaaaagat acaatttatt aaagatgatg
    15901 gaaattggca catagtaatt agaaatattg atgaaaagtt tatatcagaa ataaatagta
    15961 gagtagatat agctcaccat ggtagggggc aagtcgttga cagtggagag tttaaatcaa
    16021 aggagatttt gatacaaggt ttagatgaag aagtagcaaa acaatttaac ttagaaataa
    16081 taaggggtcg ttatcctatt ggggagaatg agactttaat agatgaggat gcagttaaaa
    16141 gctttaatct aaaaatagga gattttatca aaataaaatt agcagatggt agaaataaaa
    16201 attataagat agtaggtgaa tattcatcaa tatcaagctt aagagctaca gataaacatg
    16261 gattaatact ttcaatattg ggtacacaag ctattaaaaa atcagaaacg tacgattatt
    16321 attatattac atttaaagaa aaggttaata ttagaaaagc cacttctgat attaaaaata
    16381 ccttcaattt aagtgataaa caaatcggta aaaatgaaag attactttct attataggtc
    16441 aaggcgactc tgacttttct aaaccactgt atttaacagc aggattactt tttgtacttg
    16501 ttttagtagc aagtattatc atgatatata atagttttaa catgagtgta attgagagag
    16561 ttaaattttt tggtctgtta cgttgtctag ggtcatcaaa acatcaagtt aaaaaatttg
    16621 ttgttttaga aagtttaatt ttaagtataa aagctattcc aataggctta gtattaggaa
    16681 gtcttgtaac tattatatct tctatatttc ttaagtatgt taatgagtta ttcagctcaa
    16741 tgccaatttt aaaagttagt tttattggta ttgcatttgg aataattgta gggtttttaa
    16801 ctgtaatatt atcagctatt attccagcaa aaaaagcttc ccgtgtatct ccacttagcg
    16861 ctgtacgtgg gaatctaatg tccaaagaaa ttttaaataa cgggaagaca agtaaagttg
    16921 caaatacagg aagttttaaa attgagactt ctatgggatt aaatcatgct ttttcaagaa
    16981 agaaatcttt tgtacttatg acttcttcat ttgccattag tattatacta tttttaggtt
    17041 ttagtgtgct tatagacctt atgtatcaag gacttaagcc tatgaagcca gaagctcctc
    17101 atatatctat tgttgaccct aattttaagt ctgttttaac taaggaagat attaatgaaa
    17161 taagcaatat caaaggagta aagaaagtat acagtagaat gatgacggat atagaagcta
    17221 catataatgg gaagaagaca aaatcacaat taatttctta tgaaaagtat caatttaatt
    17281 gggcaaaaaa atatctatta aaaggagaag ttaatgaaaa taaactacag tcagaaaacc
    17341 ttgcattagt agaagagggg catggatttg atataggaga taagatatat attaaaaata
    17401 gaaatgctga aaaagagtta gaggtaggag gtatactttc ctcaattcca tttgaggcta
    17461 aagataaata tattggaaat attataacat ctgaaaatac ttttacgaat ataacaggta
    17521 taaagggtta tataataatg gatattcagg ttgataaaga tactcctgga gatttaattg
    17581 aaaatataag acgtgtagta gatgataact tagaggtcag agataaaaga caaagtaact
    17641 tagaggttaa aaattcgttt tatactatgg caacatttgt atatggattt gtgtttatta
    17701 ttgcgattat aagcatgttt aatatcataa atagcatgaa tataagtgtt acaagtagaa
    17761 agaattacta tggtgttatg cgtgcagtag gcatatccaa cagacaatta agaagaatgg
    17821 taatagtaga ggcaggttca tatgcaatga gtggttgttt attaggtagt atactaggat
    17881 taatgttaca taggtatatg tttatatcgt tagtaagttc aatgtttcat ttagagtggc
    17941 aagtaccttt taatttacta cttatcactg tactaactat gataataata actttattat
    18001 cagtaagaaa accagtcaaa aagatttgtg aattagatat aatagactca ataaatgcac
    18061 aataaatata gctgttgatt taaattaaaa aaacgaagta aaaatgaaat agaaaatatt
    18121 tttcattttt acttcgtttt ttatattata aaatttttgt tatgttacaa aatttctata
    18181 aagatgatgt gaatctaaac attatatgac aacgtgagca tttaagttaa aatattttaa
    18241 atatactatt ttagataagt tccttcttca tcttgataaa ttaatccttt tttcataagc
    18301 actccaagag ctcgtttaaa atttttctta cttgaattaa ataatataga aatatcttta
    18361 gggtcagatt tatcattaaa tctcatatat ccatcagaac cctctaaata acttaaaatc
    18421 ttattttcta aagtatcaag ttcatccttt ctatttcctc taggagaaag accaagtttt
    18481 ccatcttcat atatttttat gacatgtaaa ttttcaagaa catcaccagc ttttagttca
    18541 gtaaagtact cattatgaag tattacacca gcatatttat tgtctacaca tatcattgca
    18601 gaattattag tttgaaatcc atatacaact ccagttacaa tatctcctac attgtaagta
    18661 tggtctgtta ataaatatga atctatatct gtagtagcag caattctttt gcttttgtct
    18721 aaatatatat aaaataaata tttttcgtct ctaaatagtg gataagtctt agctttaaaa
    18781 ggaactagta tgtctttagg aagtcccata tctataaaag taccaatgtc cgtattatca
    18841 actactttta aatgtgctac atccccaacc ttagctaaag gcggtattaa agttgcagca
    18901 gttctgtcat cagaatcttt aaatatgaaa gcatttactt catcacctat gtttatttca
    18961 ttttttccga ttaatctatt atgaagaagt atatcgtctt ttgtgttatt agtttgtcca
    19021 tccaaaaaat atccaaattc agtttttctt tttactgtaa gtttattaaa atcacctatg
    19081 tttatcaaat taaaatcatc ctctcatttt atatttataa tttagccata atatacttat
    19141 gtagtagttt gtcacagtat ctatattagt attagtgaaa tttacaataa ataatatagt
    19201 tatttatgta tttttcaatt ctctagcttc ttatatagta taaactccaa tttctttttt
    19261 gtctttaggt tttactaaaa tttttacaaa ataagttaaa aagtattgaa ataaaatttc
    19321 ttttaaagta taattggtat ataccaaaat tggtatatac catatcgaaa agtggtgact
    19381 atatgaaaat agtaaataaa tctagtaata taccattgca tactcaatta agttcaataa
    19441 taagagagat gatagaaact ggtgagctta aagagggtga tgcaataatg ccagaaagag
    19501 aactttgcaa catacaaaat gtaagtcgta tgacagtaaa taaaaccatt gttggattag
    19561 tcacagaagg acttctttat agggtacaag gtaaagggac attcgttgca aagcaaaaaa
    19621 agaagtatca gttttcaaat gtaaaaggtt ttactgatgt tatgaaagaa aagggagtaa
    19681 acataaaaac ggatatatta tcctttgaga tggaactacc agatgattta gttaaaagaa
    19741 agcttggaat aagtgataat actacaaata tatataagat tgtcagactt agatatactg
    19801 atggagagcc atttggatta gaaatagtat atctatcaga agaaatgtgt aaaggtctaa
    19861 caaaagggat attggataat tcttcattat atagggtttt aaatgaaaaa tatggctata
    19921 agatacaaaa agctgaacaa gtaatggagc cagtaatact ttcagatgaa gaaagtaagc
    19981 ttcttgaaac agatgaaggt gctttagctc ttaaacttca tagaaattcc tataatagag
    20041 aaggtagccc tatagaatat accatatcaa tatttagaac agataaatat caatatgaaa
    20101 tagtattgag tgagtaggtg aataatatga aatgtttaat taatggaaag ataatattaa
    20161 aaaatcaaat cttggaaaat aaagtattag tatttgatga aaaaataatt gatatagctg
    20221 atagtgtacc aaaagattgt gaagttatag atgctgatgg aaaatatata tctccagggc
    20281 ttattgatat tcatatacat ggaaatatgg gaaaagatac aatggattcg actgatgaat
    20341 ctattgaaac aatttcaaaa tcaattatga gacatggagt aacatctttt cttccaacta
    20401 caatgactat ggataaagaa catgtttatg atgcacttga agttattaaa aaagcccaaa
    20461 atagaaagct tgaaggggca caagtattag gagcacattt agaaggacct tttataaatg
    20521 agaattataa gggagcacaa aatgaaaaat ttataataaa ttcaaaatac gaatttataa
    20581 aagaatataa agatgttata aaagttataa cttatgctcc agaaaaagat attgattttg
    20641 attttactag agaaataaaa agatgtacag atatagtttt atctattggt catagtaatg
    20701 caaattatga ccaagctaaa gaagctataa atttaggagt aactaatata actcacatgt
    20761 tcaatgcaat gactggttta aaccatagag acccaggcgt tgttggtgca gcacttacaa
    20821 ctaatgtgta cagtgagcta atagcagata caatccatat aaataaagat ttatttcaat
    20881 ttatactaaa taataaaggg aaagaaagac tgattttaat aacagattct atagaagctg
    20941 gtggtcttga agatggcaat tattctcttg gaggtcagaa agtcatagtt aagggtaatg
    21001 aagctagact tgaaaatgga gcattagctg gaagtgtgtt gtctttaaat aaaatggtat
    21061 ttaacttctt agataataca aaccttaaag tgaatgaagc aattaattta gcttctttaa
    21121 atccagcaac atctttggga ataaatgaca aaaaaggaag cttagaaata ggtaaggatg
    21181 cagatatagc tgtatttgat gagaatttag attgtaaaat gacactttgc ttgggggacg
    21241 tagtatataa aaatatatag tatacaaaac aaactttaaa gacagtaaaa acaatataaa
    21301 aaataataaa ttaaacttac attagttaaa gtagtagtta aaatttagga ggaataagat
    21361 gagagtatta gtgtgtaaag attatgatgg aatgagtaaa aaagccgctg aaatgatagc
    21421 agctcaaata gttttaaaac caaattcaat actgggacta gctacaggaa gtacaccagt
    21481 aggtatgtat agagatttag taaaaaaata caatgataat atagtagatt tttcagatgt
    21541 gatgagcttt aatttagatg aatattataa gttaccaata tctaatgacc aaagctatga
    21601 ttattttatg aaagagaatc tatttaatca tgtaaacata aagctagaaa atactcatct
    21661 accaaatgga atggctgatg atattgaaaa agagtgtctg aactatgaag cttctataga
    21721 tgcagctggt ggtatagatg tacaagtact tggaataggt cgtaatgctc atataggttt
    21781 taatgaacct gatactaaat ttgcaaaaag aactcatgta gttgaactta cagagtctac
    21841 aatagaagct aatgcaagat tctttaaatc aagagaagat gttcctaaaa aagctgtaag
    21901 tatgggaata ggttcaatat tgaagtctaa gaaaatattg cttttagctt caggagaaga
    21961 aaaagctgat gcagtatata atactgttta cggagatata actccagaag ttcctggttc
    22021 tatacttcaa cttcataaag acactatagt tatagtagat gaagcagcag caagtaaact
    22081 gaatccaaaa gattataaat tagtataaga atagaatgta gtttaagtga aaaatagcta
    22141 tcattgaata atcaaattta tttaatgata gctattttta tttaaatttt ttacttcttt
    22201 ttatttaaat tagttattat tgtttaaatt acttgcttat tactttttac gaaaactcgt
    22261 tatcaagaag tatccgaata agatgttaaa aattattatg tttagtggga ttctatcaag
    22321 cataataaaa ttacctataa ttgggatatt accaattgga aacataaaga agaatacagg
    22381 catcagtgat agtaaaaaag atggaaaaca aagaagaagt aatcttggaa tatcaatata
    22441 ccaactacca tcctttttgt attctctaat caataattca gttcctagta acaaaccaat
    22501 accaccagag gaaattaaat taataaaaat gtatctttta aagtcttgca tataaccagt
    22561 gtgaaattga gtatagaaat cataatttaa tttaacggct aaaaaatata taagcaatag
    22621 aatgattaaa aataaaagat atctaagagc ttttttcatt ttaaattctc ccttcagaaa
    22681 tactttattc agttttattt tgttggaatc tgattttatc atttagttta agaaatccat
    22741 aatattagtg aagaatgaaa taagtattat atatgaataa atatttttag aattaaggtg
    22801 aactgtagtg cttaatataa ctataatatt aattgtttga atattcaata aaaagtttaa
    22861 aaaatatcaa ttttgatata aactgaataa atacagttga atattaaatt attattttat
    22921 caaaaatatg caaagtacta caccttcaaa tatcacctaa agtaattaaa aggcaattaa
    22981 agtgaagtag ttcaagtatt tctaaaatga gtttaataaa ttctataata atttattttt
    23041 tgccttaaaa ttgccttaaa atccaaacaa ttttaaaact aatgtactat aaaaagctgg
    23101 tataatttaa atatagtaaa aaataagatt tatcttatct attgggattt aattatttaa
    23161 tcaatatatt tataccattt aatttaaata aaaagtctat taaggagtga gcttatgagt
    23221 aagttaattt atatagcaga tgatgaagat aatataagaa atttggtaaa gacattctta
    23281 aaaaatgaag gtcatgatgt tatggatttt aaaacgggtg atgaactttt agaacaattt
    23341 aatataaagg agtgtgactt agtaatacta gatataatga tgcctggctc aagtggtttt
    23401 gaagtatgta caaaacttcg tgagaaaagt acagtcccaa taatcatgct tactgcaaga
    23461 gatacagata tagattatat tacagggata accttaggta gtgatgatta ttttacaaaa
    23521 ccatttagcc caatgtcttt ggttatgaga gtaaaatcta tatttagaag gatagagttt
    23581 gaaaagaaac aaaattatga taaatatagt aactcgatag atatggaatt aaaatttggt
    23641 gatgtaataa taaataaaaa gaataaaatc gttacaagta aaaatgtaaa tatagacctt
    23701 acaccaaatg aatataatct attgacttat ttgtttgaaa atatagatag agcagtttcc
    23761 agagatgaat tattaaacaa aatatgggga tacgatatag aagttgagac aagggctgct
    23821 gatgatacag tgaagagatt acgtaaaaaa attttagata caaatatttt aatagaaact
    23881 gtatgggggt ttggatttag gttgaaggag aaaagttaag atgaataatg gaaatgacaa
    23941 gaatagaatt ggaaagatta aatccaaatt taaattaaag ttctcaaaga aaaatataag
    24001 cataaaaact aggattttgt tgagcatact ttgtttgata attatagtat ttatgataat
    24061 attagtatct tttaatgtat ttcttgatac atatataaaa acaactgcga atgaagaatt
    24121 gacgaagtct acagaaatag tagaacatat ggatagtaat tttagaccta ttaagccacc
    24181 acaagatgat ggaaagcttc ctcccaaagt attatctaat tttatgagaa atgtacaaga
    24241 taaagttagg atggcagaaa cacaatcaga tgctgatgct atggtagttg attctaagta
    24301 taacctgata tttccaacaa gagaggatga tttcttaaaa aatgttgatg agatggagct
    24361 gattagggag tgtattcaaa atgaaaaatt agatttaaat tcagatgaaa atacacggat
    24421 atctaccaga aataaggatt attatatttc aactgtaaaa attactagta tatatggtga
    24481 acaagacaaa tatcttatac tttttataga catatcaaaa actctaaatc tagcacaaaa
    24541 gataaatatt gtattaatat ctgtaatgtg ttttgctgga atattagcaa tatttacagc
    24601 tgtgatttta tctgagaaaa ttgctaaacc gataaaagag ttgtgtgagt ttgcaaaaaa
    24661 aatgggtcaa ggagatttca aaagaaccta ctttgacttt tcagataaag aattagtaga
    24721 gctctcaact gttatgaata agagtgctga gtatctggat aagtatgata atgaacaaaa
    24781 aatatttttt caaaatgcat ctcatgaatt aaggacacca ctcatgtcta ttaaaggtta
    24841 tgcagaagct ataaaatata atgttataga tagtaaacat gcaagtgata taattcttga
    24901 agaaagtgat aggttaagtg atatggttga agatttatta tacatttcta aaatagataa
    24961 tataactaaa gattttgaac ttgtagaatg tgatttaaga gaagtatttt caaattgtgg
    25021 agctaggcaa aatgtaagag ctataaataa ggggataaaa tttatttatg aatttgatga
    25081 agaaatggtt ttatttgaat gtgatgaaaa aaacatttcc aaagcattta tgaatcttat
    25141 agaaaatgct ttgagatacg ctaagagtga aataaaaata gtgtgtaagt ataatcaaaa
    25201 aaatatagtt gtgataatag aagatgatgg aattggaatc aagaaagaag atttacctca
    25261 tgtttttgaa agattctata agggagtagg gggaaatcat ggaattggat tatcaatagt
    25321 taaatctatt gtaaataagc atggtgggcg tatatacgta gaaaatggca aaaaaggagc
    25381 taagtttact atattattta aattgtaaaa aaattagaga aaatataaag atgtttgaag
    25441 ttgtttttta tatatatctg ttgtatactg ttgaaaaaag aacaatatta agctgacata
    25501 tattacaata attgacattt aaagtaaatg cttaaatata gatacgaaag gaaaagtaaa
    25561 atctgaagtg tatttaggtt taagtaagct tcttatttta taaaaaatat gtatgaaaga
    25621 acagctgatt tttaatatta caaatttagt aacggttgta tttgaagctt ttgtcatcca
    25681 tatgttttta tcagactttt taggtcagaa agaagggtat ataaaagttg taagatatgc
    25741 taaactgggt tttataatat gtttaggatt ctgtaattta ataactttaa accctaaaat
    25801 cacaatgcct ttaattttta tccttatttt ttcaacatca tttttatata aaggaaattt
    25861 aaagacacga ttgtttacta ctgttttatt aagtatattc tttatactgt cagaaattgt
    25921 agttacaagt atttttgttt tatttgtaaa agaaggcttt gaaataatgc ttgaaaataa
    25981 tagtataaga gtattggcga caattttatc taaaattgtc tttttgttga cttgtaaaat
    26041 tatttgtcta tttaaaaaag atgtacattt agatatgccc ataaagtatt ggctcccttt
    26101 atttttaata cctatattta gtttatttct gtctgtttct atctttgatg taagtaaatt
    26161 ttttagtctt gaaagtttaa aatttctaag tttaatatct agtgttggca ttttatatat
    26221 taattttatt gtattttatt tatttaaatt tattatagat aaaactaaac tatctatgaa
    26281 atatgaaata cttgaaaaag aaataattca taaggaagaa ttaagactta gtaatgagtg
    26341 ttataaaatt attgttgaac agactgattc tgtcgttttc gaatggaata tcaaagaaaa
    26401 taaatcattt gtatcgcagg cttggactga aaaatttgga tataacaatg catgtaagaa
    26461 tatttttaaa gaaattaaag ataaagactt agttcattct gaagataaag ctatatttga
    26521 aggttttctt gaaagtatta aaaaaaagaa tatacacagt caagcagtat atcgtttaaa
    26581 aaaatctaat ggtgaatata tttggtgtag aacatcaatt acaagcattt ataatgatga
    26641 aaatgaactt ttaagagttg taggagtaat tgtagacgta gatagtgata taaaaaaata
    26701 tgaagagtta agaactagag cagaaagtga ctcattgacc aatatataca ataaaggtac
    26761 atttgaaaaa ttagtggaag aaactatagt tatgaataca ggtgataaaa aagatgcatt
    26821 attcattatt gatttagatg attttaaaga aataaatgat aattttggtc atccatttgg
    26881 tgattttgta ctaaaaacat ttgctgataa gatacaagct agttttggta ataaagactt
    26941 agtcggaaga attggtggag atgaatttgt tgtatatatg caagattatg ttacagaagt
    27001 aaatttgcat aaaaaagcta aagaattaaa tagagtactt tcggataatt acactgattt
    27061 gagttttagt tttgattctt ctgtcagtat aggtattgca aggtatcctc aagatggaac
    27121 ttcatttttt gaattgttta aaaatgcaga tagagcactt tatagcataa aagcatcagg
    27181 taaaaattca tattgtttat ttgaagaaga actatatgta cagtgaattt gaatttagat
    27241 tttatgtgag agtaagattt atatatactt gtggctgtct gatttataaa atatgttact
    27301 ttgtatattt ttatatgttt cagtaaggtt aattaacata agcaaaggtg taagtaaatt
    27361 tatattagtt taagtttact tacaccttta taaaaataaa ttttgtaaat ttaaacagtt
    27421 ggttttataa ggattttta
//
LOCUS       DAELSY010000039        27172 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.039, whole genome shotgun sequence.
ACCESSION   DAELSY010000039 DAELSY010000000
VERSION     DAELSY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27172
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.039"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(359..772)
                     /locus_tag="KPG09_001493"
     CDS             complement(359..772)
                     /locus_tag="KPG09_001493"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBG0966112.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(813..1943)
                     /gene="rodA"
                     /locus_tag="KPG09_001494"
     CDS             complement(813..1943)
                     /gene="rodA"
                     /locus_tag="KPG09_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBG0966113.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(2028..2312)
                     /gene="minE"
                     /locus_tag="KPG09_001495"
     CDS             complement(2028..2312)
                     /gene="minE"
                     /locus_tag="KPG09_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBG0966114.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(2330..3127)
                     /gene="minD"
                     /locus_tag="KPG09_001496"
     CDS             complement(2330..3127)
                     /gene="minD"
                     /locus_tag="KPG09_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBG0966115.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(3151..3834)
                     /locus_tag="KPG09_001497"
     CDS             complement(3151..3834)
                     /locus_tag="KPG09_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBG0966116.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYENESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(3932..6910)
                     /locus_tag="KPG09_001498"
     CDS             complement(3932..6910)
                     /locus_tag="KPG09_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBG0966117.1"
                     /translation="MEENKQVDRLKIIKIVMSVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKMNVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(6922..7410)
                     /gene="mreD"
                     /locus_tag="KPG09_001499"
     CDS             complement(6922..7410)
                     /gene="mreD"
                     /locus_tag="KPG09_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBG0966118.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(7425..8318)
                     /gene="mreC"
                     /locus_tag="KPG09_001500"
     CDS             complement(7425..8318)
                     /gene="mreC"
                     /locus_tag="KPG09_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBG0966119.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKDMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(8328..9338)
                     /locus_tag="KPG09_001501"
     CDS             complement(8328..9338)
                     /locus_tag="KPG09_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG0966120.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            complement(9459..10118)
                     /gene="radC"
                     /locus_tag="KPG09_001502"
     CDS             complement(9459..10118)
                     /gene="radC"
                     /locus_tag="KPG09_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBG0966121.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(10163..10747)
                     /gene="maf"
                     /locus_tag="KPG09_001503"
     CDS             complement(10163..10747)
                     /gene="maf"
                     /locus_tag="KPG09_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBG0966122.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(10967..11938)
                     /locus_tag="KPG09_001504"
     CDS             complement(10967..11938)
                     /locus_tag="KPG09_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBG0966123.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(11954..12529)
                     /gene="rsxA"
                     /locus_tag="KPG09_001505"
     CDS             complement(11954..12529)
                     /gene="rsxA"
                     /locus_tag="KPG09_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBG0966124.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(12544..13137)
                     /locus_tag="KPG09_001506"
     CDS             complement(12544..13137)
                     /locus_tag="KPG09_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBG0966125.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(13150..13719)
                     /locus_tag="KPG09_001507"
     CDS             complement(13150..13719)
                     /locus_tag="KPG09_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBG0966126.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQENNEL
                     RKAVLPEAKEFKEMDKSTYKDLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(13719..14696)
                     /locus_tag="KPG09_001508"
     CDS             complement(13719..14696)
                     /locus_tag="KPG09_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBG0966127.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(14719..16056)
                     /gene="rsxC"
                     /locus_tag="KPG09_001509"
     CDS             complement(14719..16056)
                     /gene="rsxC"
                     /locus_tag="KPG09_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBG0966128.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(16272..17216)
                     /locus_tag="KPG09_001510"
     CDS             complement(16272..17216)
                     /locus_tag="KPG09_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966129.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(17327..18601)
                     /locus_tag="KPG09_001511"
     CDS             complement(17327..18601)
                     /locus_tag="KPG09_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG0966130.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSNQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKSKNNGWYKVKLSSGTIGWVSASYISETNEGTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(18733..19104)
                     /locus_tag="KPG09_001512"
     CDS             complement(18733..19104)
                     /locus_tag="KPG09_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0966131.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(19332..19931)
                     /locus_tag="KPG09_001513"
     CDS             complement(19332..19931)
                     /locus_tag="KPG09_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966132.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(20132..20344)
                     /locus_tag="KPG09_001514"
     CDS             complement(20132..20344)
                     /locus_tag="KPG09_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBG0966133.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDISIEKLKEVIAELGFELKGIE"
     gene            complement(20417..22132)
                     /locus_tag="KPG09_001515"
     CDS             complement(20417..22132)
                     /locus_tag="KPG09_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0966134.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEEARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANINSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEKKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(22132..22686)
                     /locus_tag="KPG09_001516"
     CDS             complement(22132..22686)
                     /locus_tag="KPG09_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG0966135.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(22679..23281)
                     /locus_tag="KPG09_001517"
     CDS             complement(22679..23281)
                     /locus_tag="KPG09_001517"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBG0966136.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNISIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNSLTHPRIREKIISEIEELR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSKQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(23290..25938)
                     /gene="polA"
                     /locus_tag="KPG09_001518"
     CDS             complement(23290..25938)
                     /gene="polA"
                     /locus_tag="KPG09_001518"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBG0966137.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFKINR
                     LEIDGYEADDIIGTVSKKAENNGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPAQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMVFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDTEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETIPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            26257..26339
                     /locus_tag="KPG09_001519"
     tRNA            26257..26339
                     /locus_tag="KPG09_001519"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26291..26293,aa:Leu,seq:caa)
BASE COUNT         8247 a         5196 c         2993 g        10736 t
ORIGIN      
        1 tatattagta tttatagatt aaatactaat atacagctat ttatcaaatt ttaaaaataa
       61 atttttgttt tattaatgtt gtcgtaacaa catttacttg gttgaaatag tattacctat
      121 aagtttaaat atatataata atagacttaa ttctatttca tattattaaa atagagtaat
      181 agtttaaatt ctactactct atctataatc tatttcctaa ttatttaatc tcaaatttag
      241 ctcctcatca tttaaatctt tcataagatt tctacaggta ctaggtttat tgcaacagaa
      301 aaactagggc attaaacact tatatatctg tttctttaga ctgtaatatt aatatttttt
      361 aaatacgttg acttttgctt tttctaactt ctctccatga tagttctcct ctatcaagag
      421 cttttataaa tatttctgct gaagctagat ttgtagctat tggaacatga taaacatcac
      481 aaagtcttat taacgcttgt atatctgttt catgagcctg agatgtcaat gggtctctta
      541 aaaaaataac taaatctatt tcttgtgtta ctattaaaga acctatttgt tggtctccac
      601 ctaaaggacc agatgctaat ctatttatat ttaattcagt ttcatccatt attctttttc
      661 ctgtagttcc agttgcatat agtccatact tttttaaaat tgattcataa cctatacaaa
      721 atccgaccat agtgtttttc atttggtcat gtgctactaa tgctatattc atatatctaa
      781 tctcctcaat ttatgtttag atgtatttaa atttagaaat ttattttctt tcttctcata
      841 cacacattta gtactaatcc taagtttgct aaagatgtca ataacgaact tcctccataa
      901 cttacaaaag gtaatgttac tccagttact ggtattaacg caaccgtcat acctatattt
      961 tggattatct ggtaaccaaa cattcccata acaccaacca ctatgagggt tccataaaaa
     1021 tcttttgcat ctcttgcaat tgctaaaagc cttaatagga atatcataaa taagattatc
     1081 aacactgcca ttcctattac accaagttct tcacctacaa cagcaaatat aaaatcacta
     1141 tcttgtacag gtaaaaaatc ttcttgattt tgacttccat tgtaaagtcc tttaccagtt
     1201 acgcccccag aaccaattgc aatcagagat tgcataactt gatagtttcc ttttagagtt
     1261 acatcttctg gatttagaaa agcttctatt cttacctttt gatggtctgc catcattaaa
     1321 tacatcagtg gcattgacac taatagtatg attattcctc tttttatcaa ctttgagcta
     1381 agacctgctg tgaacagcat tgcaaatatc atacaacaaa atacaattcc tgttcccaag
     1441 tctggttgtg caatcaataa tccgataaat ggaagagaat aaactacaac aggcataact
     1501 tcctttaacg tatttaattt atcctttttg gattcaagta tctttgcata acttagtaca
     1561 aatgttaatt tgactatttc agatgtctgc aagtcaagtg gtcctaagtt aatccacgac
     1621 ctagctccac cttttactgt cccaattcca ggtaataaaa ctatggctaa caaaataaga
     1681 cttattatat aaagcgcttt ataatacctg cctagaagat tatagtcaaa aaataatatg
     1741 actataatca ttcctatacc aagtacaaaa gctaatcctt gtttaatcaa ttgattatat
     1801 gaacctgttg aattagcatg tgttgcacta ctaagtataa caagcccaaa aataaagata
     1861 gctaatactg taactataag tttccagtct aactgtttta tcaacttaat tgtcgactta
     1921 taattaatat tgattttttt caaagaatca ccgtctttct atctttaaac atataattaa
     1981 ataaaagggg catataccaa taaggatatc ccccttttaa gcactaatta catacatcta
     2041 tcttttatat tctttagtgg tatattagca actaatgcag atacaggttt gtcatcatgc
     2101 tcacctctgc atttagacat ttttatttca agaccatcat cttctatttc tgcataatta
     2161 gctattactt ttaaaatgtc tgtttttatc atctctaaaa gttgtggtga acaatctact
     2221 ctatcatgaa ctaaaactag ctttaacctc tctttagcaa cagatttact agttttagcc
     2281 tcgttagaaa aaactctaaa taaatctaac acagatgtat cctcctttgc tacttagcca
     2341 tgctaaacat ttttttaagt ctaccaaaga atccatcttc aacttcaaga tcaagtagag
     2401 gaacttcttc atttagtatc ctttttgcga tatttttgta tgcttgacca gcaagtgact
     2461 tagaatcaag tatagctggc tctcctttat ttgttgatat aattatgctt tcatcatcag
     2521 gaacaagacc taacaaatct attgctaata tttctattat atcttgttta tccatcatgt
     2581 ctccacgctt aaccatatca tttctaattc tatttataac taatcttatc tcttttattt
     2641 catttgcttc taatagacct attattctat cagcatctct tactgctgat atttctggat
     2701 tagtaactac aatagctcta tctgccccag ctactgcatt tttaaagcct tgctcaatac
     2761 ctgctggaca gtctattatt atatactcga aagattcttt tagtttctca cacagatcaa
     2821 tcatctgctc aactgagaca gcatttttat ctctagtttg tgctgctggt aataaatata
     2881 gattatcaaa tcttttgtct tttattagag cttgcttaag cctacaagtt ccttcaacta
     2941 catctactat gtcataaact attcgatttt caagacccat tactacatct aagtttctaa
     3001 gtccaatatc tgcatccaca actactgttt ttttattttc taggcttagc gcagttccta
     3061 agtttgctgc ggtagtagtt tttccaaccc cacctttacc agatgttata actataactt
     3121 cgctcataat actaccccct atttttcttt ctatttgtct aactttgaca aatagctttc
     3181 gattacaatt cttccattac ttacaaaggc tatttctggg ctaatttctg attcattttc
     3241 ataattttct tcatcatctg gagcttctgc aatattatct gctatttgta atattttagg
     3301 attaagattt ccagcaacaa catatgcaaa ttcattgcct tctgcacctg catgtacaaa
     3361 accttctatt ttacccatta caaaaacatt acctcctgct acaaccttag caccagcatt
     3421 tatatctccc ataacaactg tatttgattt tgaaaggact tttgaaccag gtttcatatc
     3481 attcaatata actacatcac cttcaaattc aatattttca ccagatctta gggtattgac
     3541 atattttgtt ggaaaattac tattttcttc aattttttga tcttcaacaa attccacatc
     3601 aaatctagaa gtaattcctt ctttaagttc tagaatctcc atgtcagtta aacaatcact
     3661 attaatttta ctaattttag ccccattaaa aaagccaaca tatgcttcca atttgtttat
     3721 tattttttcc tgtatttcct caaatggagc ttctcttttt atatttacta ttatccctct
     3781 tttattaccc ttgaactcta ctagttcttg actacaaatt tctcttaaag acataacaac
     3841 ctccagagtg atatatcatt tatattcttc aaatacgctt taaacccttt tattttttct
     3901 atataaagct aaagtttata catagattaa gctatctatt aatttgtgac tcgaaattta
     3961 tattttcgtc agaagaattg ttaacatcat tcgtcttgtt gtcattttta ctattagttg
     4021 gctctaatcc catataagtt ccaataactt ctctagttgg taaaagtgag aatacacttg
     4081 aatcaccttg aggaattaca catacaacag ctatttctgg gtcatctgct ggagcaaaag
     4141 caacagtcca tgtaaaagaa ccataatctt gtttaaatct atctatttgg tcatctgtta
     4201 tttttggatt taattctttt atagcttttc ttagatatga agaattcacc ttatcagtgt
     4261 cctctaatct aacttttact ccatcttcta attcttcctc taatttcttt ctttcttcgt
     4321 ctttcaaatc tttattttct aatttctttt ttatttcctt ttccttagct aatgaaagct
     4381 ctttttcttt ttctgctttc atcttatcag ctagtttaat cgcatcattt aggttgacat
     4441 tataagatga catatgactt tttaaatatt catactcgtt atctgtaggt attttcccac
     4501 ttttctcagc tgtaccagtt tttgctgcta catctattgg aaaatcagca aaagcactct
     4561 tcgctgtacc ttgtcttgca accaatttca taccttttgt taattctttt aagttatctg
     4621 gatttttaaa aggtattttt tcgactttct tctggtcatt aattttaact gatgaataat
     4681 cacttgaaac agctctatca actacagata attctactaa attacctcca tttgctatag
     4741 cagctacata tcttgatatt tgagctggtg tataagcatt ttcaccttga cctatagcta
     4801 agttaaatgt atctgcagta ctccattttg caaagttaaa ataactgaat actgctaaat
     4861 ctgctacttc ttctattcta tcttctttaa cattcatttt ctttagccta ttcattgttt
     4921 cgactctacc aggagttttc ttttctgctg cccagcttac aatctcattg attcttttct
     4981 catattcttt aggatttttt tctctagtta tatcaacaaa atcatttgcc atttctcttt
     5041 ctaaatttga ctttagaaga gcttgtgttg atttcaattt atcttctgtg ctaggaactt
     5101 tacctgctct ttcttctact tcatcttgaa gccctgtatt ttggtcaagc ccaaataatt
     5161 ttgcatactc aagtatttta tttgcattca tatctatacc aatgcttttt ccatctggcc
     5221 atgttttacc tgtaccaatt gttgccatat atatattaca agactcttgt atcgctttat
     5281 ataagtttgt cataccatgg tttcctctac catggttcca tacatagtca ccaaaagatt
     5341 ttttacctaa cattataacc ccagggtcat ttatagtaaa gtttggatcc aaaccatttt
     5401 ctaatgctgc cattgaagtt accatcttaa atgaagaacc tggctgaaat tccccttgtg
     5461 taaccaagtt taaaagtgga cttcctgcta gtaagtcatt tggatttttt ggttgaagtg
     5521 ctttataatc ctctgtagaa ataccttttg caaatttatt tgggtcataa ttaggataac
     5581 ttgatgaagc taatacttct ccatttttaa catcaatagc tataagagct gctgactgtg
     5641 cttttcctgc atatgcacta attggtttgt ccccaaattt acttttaaaa gtacctcctt
     5701 tagaagcaac ttcgattatc tgttttaatg cattatctga aacttcttgt aaatctttat
     5761 ctagtgtaag atacacagta tctcctgatt taggtttttt agattcaatt tctttactga
     5821 ttctacccaa tgcatcaact ttaaccattt tataaccatc agttcctctt aatctacttt
     5881 cattactctt ttctactcct gcaagaccta ccatatctcc agtttcataa cctttttgaa
     5941 ggtatgattc tatttgagtg gatggcattt ttcctacata tcctaacata tgagaagcta
     6001 atgacccatt aggataatat cttactggtt ctactgctat tgaaacacca acaaggtcca
     6061 tagcactttc ttcaatttga gcaatcgtag tttctcctac atccttagca atagttactg
     6121 gattatattg agaatatcct tttgatttta tcaaatccct tacgaccatt atttttcttg
     6181 catcttcatc actcaaactt ttatcaattt ctaatgcatc actatttctt actttttcaa
     6241 acgcttcttt ggcactcagt ttagtttctt ttatcttata actagccaac caatctctct
     6301 tatctctttc tgctgtgaac atccatttac taactaaaat aggtggataa tatccatttt
     6361 catttagttt tgcctgtaat cttgtcgcat ccaatctttt atcttcttga gataatattc
     6421 cagctgatat aagtttgtct actaagtaat aaaaactttc cttagcatta taatcatttg
     6481 gaataccatt ttctgattta tattccctaa tatctctgtc atatgtataa tagtactttc
     6541 cattttctac atatatagga aactcatcta catattcttc accatttctc tccagcaaat
     6601 ttataagttt taatgatatt tcatttgctc ttgaatgctt atttgcatct ttcttattaa
     6661 tatcgttacc tgaaacttga acagtaaata agtttttatt tcctgccaac agtcttccat
     6721 atctatcttt tatttctcct cttggagctt ctattgccaa ctttttatat gttttattct
     6781 cagctaactc attgtaatat tcatatttaa atgtagtcat atagattatt tttactaaaa
     6841 tagctaaaaa tatcacgctc atgactatct taattatttt tagtctatca acttgtttat
     6901 tttcttccat gaaatcacca actaatcttc tttaagtttt aaaactgctc ttttagataa
     6961 gcgatatatt actaaaccaa ttaaactatt cactattggt gctactagta gaccttttac
     7021 aaaaagtgtt gatatgttgt agctttgata aattatacta gatgtaccta tattgacaat
     7081 tgaatcaatt aaactgccta taaaaattaa aacgaaagta ataatataac tacctttata
     7141 tagctgatct ctcatgtatc caattgcata tgcaataata aacaaaatta atgcatttac
     7201 accaaaaatt ccaccaacgg ttatgtcttt aataagccct attattgctg ctataatccc
     7261 accttctaat tcatctaaat aaagagagat tatggttaca tatataagaa ctaaattaaa
     7321 gcttatgcca aaaatatcta tataatttgt tatactattt tctatagtca taagcacaat
     7381 ccccaacaga caaagtaaaa cttttttcat tgtttttatc ccctttatcc tatatttctt
     7441 ggttctatga ctacaacgtc atttatattc ttaaaatcaa cataaggttt tacaactaca
     7501 tattttaatg acttatcttt atcatcaacc accttttcaa cttcgccaat tggtatacca
     7561 tctgggaaga atccaagacc tgatgttgtt attatatctc cttgaattac attatatgat
     7621 gagtcaaaca tatatccttg caaatatcct ttactattat aactatcttt attatctaaa
     7681 gtagtatttt gagtaattgt acctttagca ttggcatcct ttgctagttt aaagcttaca
     7741 gatgccttag aatccaatag tgaaattgcc ttactataat tgttggaaac ttcataaact
     7801 atgccaacta acccactacc attcataact atactttctt tttttacacc tgaatttttt
     7861 cctgcaccta tgacaaaact ctcataccag tttccatcat ttttaccaac aacacttgct
     7921 gaaatacttg tcgccttata ctcttcttgt acaaagttca gtgtcttttt taactcttct
     7981 agcgattctg ttttatctag tttggcattt aaagctatca tatctttttt aagtttttca
     8041 ttttcttctt ccaatttttt taccttttta gaattatctt taaaatttgc tacatcttta
     8101 aaaccatttt tgataaaaga aaatccatca tttattccct tacctacact tgtaatagca
     8161 tctgaagcta ctcctaaatt tactggacta ccactagaaa atttaccaat tgatataccc
     8221 acaattccaa ttaaagtgat agcaactacg cccgttgcta tcactttaac attaatcctt
     8281 tttttctcat ttttgttctt gtcaaatctc aaggccatca ccaacttcta attcttagta
     8341 ttcatcatta atactttttc aaagatttct tggtcttcaa cagatttacc tgttccaagt
     8401 gcaacacaat caagaggtgt ttctgctatt tgtacagaca taccagtttc ctgtgtaata
     8461 agcttatcaa gacctctaag caaagcgcct cctcctgtta gcattattcc gttttccatt
     8521 atatctgagg ctaattctgg tggagttctt tctagtgtag attttattgc atctactatt
     8581 gaacttacag gttcttttaa tgcttctcta acttctgtag agcatatttc tattgtttta
     8641 ggtaatcctg agattaaatc tctacctctt atttgcatat ttatttcttc atcatcttta
     8701 aatgtagaac ctatgttaat ttttacattt tcagcagttc tttcacctat cataagatta
     8761 tattcttttt ttacataagc cactatagac tcgtcaaatt cgtctccacc tattcttata
     8821 gatttagcag taacgattcc tcctaaggat attacagcta tttctgaagt acctccacca
     8881 atatcaacta ccatattacc ttctggttct tcaactttta atccagcccc tatagccgct
     8941 gccattggct cttctataag gaatgcatct ttagctccag cttgtcttgc agcctcttct
     9001 atagctctct tttctacttc tgtaactcca aaaggaacac atatagctat tcttggactt
     9061 acaacacctt ttttatcagc cgctttttgt ataaaatagc taatcattga ttgagttatg
     9121 tcaaaatcag ctattactcc atctttcata ggtcttatag ctactatatt tcctggtgtt
     9181 ctacctatca tttttttggc ttcttcacct actgctaata cctctttact atcatctctt
     9241 attgcgacaa ctgatggttc tctaacaaca ataccttgtc cttttatgta aactaatgta
     9301 tttgcagtcc ctaaatctat tcccatatcc tttgtcatct tattaaaact aaaaaaacct
     9361 tttttttctt tctttttttc cttagccatg attttactcc ttcctactat taagaatcat
     9421 gtttaaaaca ttctcctatt attttatcac atataaagtc aaattatcat attttcttta
     9481 aagctaaagt ataatccatc tccaataatt atatggtcta ttatttctat tccaattatt
     9541 tctccacatt taataagtct tgatgtgata tttttatctt ctcttgatgg ttctacactt
     9601 cctgaggggt gattatgcat cactattatc ttacttgcac ttcttcttat agcttcttta
     9661 aaaacctctc ttggatggac taatgacgag cttaaagtcc ctatagatac atctacatca
     9721 gatattatct cattttttgt gtttaaaaga actgctttaa aaacctcttt ttttaaatac
     9781 cttaggctat ccatataata cctctgtata tcccaaggat tccttatttt atactttata
     9841 ggtttaaaac tagctactct agagcctagt tctaaagctg ctttaatttg ggttgatttt
     9901 gataacccaa ttccatctat attacacagc tcctctattg tcatatcttc taaatgtcta
     9961 ataccctgaa tatctctatt tattatatag ttggctaatt caatagcatt tttattcttg
    10021 ttgccagttc ttaaaagtat agccaacaac tcagcatttg ataagctttt tacgcctttg
    10081 gcaagcatct tttctctggg tctttcctct ggtgccattt cttttacctt aatggttgaa
    10141 ttaaatgact ttttcagata aatcacactc cataaaaaag atttatacta aaatattttt
    10201 ttaataggtc gcctaacctt gaaattggaa gaccaactat attaaaataa tctcctctaa
    10261 tttcttcaac taacaaagct ccataaccct gtataccata ggctcctgct ttgtcaaatg
    10321 actcattagt tttcagataa tcctcaatat cctgctctga taacgtttta aatttcacat
    10381 tgctgattac atagtcaatt acctttttat cttcgcacaa gtttattaag ctaatacctg
    10441 ttatgacttg atgctctctt ccagatagtc tttttaacat atctcttgca caagactcat
    10501 cttttggttt tcctaaaata gcattgtcta aaacaacaac tgtatcagct ccaataacaa
    10561 catcttcatt atgttcagat gctacagaca tacttttttc aaaagctaat ctcataacta
    10621 aatgttttgg tgtttcacct tccaatataa tctcatcaat ttcattttta attatatcaa
    10681 atcttacatt cgtattctct agaatttctt tccttcttgg agaggctgat gccaagatta
    10741 tattcattta ttcacctcat ttgtacgtaa tttgtaccta tttagtctat cactatattg
    10801 taaaaaattc aacagaaatg tcgaaccttt tttgttacac tattgttata tttattacca
    10861 ttagaataaa tatacataaa atagaggtat ggaaattatt ccatacctct gttttatgta
    10921 ctcattagag atatggaaat tattccatat ccttattttg tccatattac aaaattttaa
    10981 tagctttctt tggacatttt tccatacata aatggcatcc tacacattta tctgcatcta
    11041 ccttatgttt ttcttttaat tctccctcta ttgcatcaaa cttacattgt tttttgcaca
    11101 ctgtacatcc aacacaaagt tcttggtcaa tagtaacttt ctttttctta gttatgtcac
    11161 ctgatattac ttttgtaggg cattttgcta cacattgcat acaccctaca cacttattag
    11221 ggtctatctt agctattttg tcttcaaata ttattgcatc aaatttacaa gcttttacac
    11281 acattccaca gccaatacat ccagcagtac atttttcctt aacagcttta ccaaactctt
    11341 tgctattaca ctctacaact acttcttgac tttctggttt tgtgattatt atacccttag
    11401 gacaaacttc tttacatttt ccacaattta cacacttttc ttcatctatt actgctattc
    11461 catcaactat agaaatagca tcaaatttac aagcatcctt acatgttcca agacctaaac
    11521 atccaaattt acacatctta gcacctgagt ttaaaacatt tgctgctcta caatctgata
    11581 ttccttcata ctcatattta tcttttgcac ttgaacaagt tcctttacaa ataacttttg
    11641 ctacttgttt ctcacctgct tcagcactta ttcccattat ttctccaact tttgcaccaa
    11701 catctgctcc acctacaggg caaccattaa ctggtgcatt gccttcaact atcgcatttg
    11761 caagacctcc acaacctgga aatccacaac ctccacagtt ggctcctgga agtactccta
    11821 gtatagcttc cactctctcg tctacttcta cagcaaactt tttagatgcg aaatctagta
    11881 ctatcccgaa gattaagccc ataattccta aaactaatac agctgtaagt atcaccattt
    11941 tcacccctct tttttatagc tttataagtc ctgtaaatcc taagaaagct attgacatta
    12001 aacctgctga tataagcgtt atagggaaac ctttaaatgc ttctggtata tcagctaact
    12061 ctagtctttc tcttatacct gcgaatatta ctattgctaa agtaaatcca gctccggctc
    12121 caaatccatt tattattgtt tctactaaat tataactttt ttgaacatta actagagcta
    12181 taccaagaac tgcacagttt gtagttataa gaggtaaata aactcctaat gcttgatata
    12241 aagatggact catcttttga attaccattt caacgaactg aactatagaa gctataacca
    12301 atataaaagc tattgtttgt aagaatccag ttccagtttt tactaataaa atctgtataa
    12361 aataagttat aattgaagct agagttaaaa cgaacgtaac cgcaactccc atccctactg
    12421 ctgtatcaac cttcttagaa actcccaaga aaggacatat acccaagaat tgagatgtta
    12481 taacgttatt aacaagaacg atacttaaaa ataaaagtat taaattcatt tattacacct
    12541 ccactatgct tttttacttc ttaatttatt aaatcctgca aataagaatc ctaatgttaa
    12601 gaatgcacct ggtggtaata taaatattaa tactggttgg aaagatgctc caaataatgt
    12661 cattccaaat aaactaccat ttccaagtaa ttctcttact gcacctatta ttgtaagtgc
    12721 aacagtaaat ccaagacctt gacctacacc atcaactatt gaaggcatag ctcctgtttt
    12781 gaaagcaaaa ctttctgcac gagctaatat taaacagtta acaactatta atggtatata
    12841 tataccaaga gccttatcaa gagcaggaac atatgctttt aaaagcatac ctactattgt
    12901 aacaaatgta gctataacta ctataaatat aggtattctt atcttatctg gtgtaatttt
    12961 tcttattaaa gatataacta aatttgcaca tattaaaact gctgctgttg aaagacccat
    13021 accaattcca tttattgctg aagttgtaac ggctagtgtt ggacacatac ctataacttg
    13081 aacaaatgtt gggttctcat ctattagccc atttttaaat actttagcta aattcataat
    13141 taaccctcct tatttattaa gagcactatt gaaaacatct atagcagcat ttactcctgt
    13201 agtaactgct gtagatgtaa tagttgcacc tgatattgct aatatttgat tatctgctgt
    13261 tgtacttcct tttgctactt caagaggctc agcagttttt cctttgtatt gacccttaaa
    13321 tgcatcatcc ttagcttttg ctccaagacc tgctgtttct gacatattac caatgtcaac
    13381 acctgtaact tgaccttcag atgatatacc taccataact tcaatttctc caccatatcc
    13441 acttggttta gcttttaaag tatatccaac cacttcagaa ccatctaaac cttcatacac
    13501 ttcagctata agaccatctc caaggtcttt atatgtactt ttatccattt ctttaaattc
    13561 ttttgcttct ggaaggactg cttttctaag ttcattattt tcttgtatat ttctttgttc
    13621 tattactgga gctgtaacct gattagtagc tccaagagct aatgcagcta ttgcacttat
    13681 agcaagtaaa gtaccaccta atctcaccat actgttcatt atttagccac ctccccaaat
    13741 actctatttc taacaaactt atctataagt ggagcaagac agttcactaa tagtattgag
    13801 taagatacac cttctggata tccaccatac attcttataa cagttgttat aagaccagct
    13861 aatacagcgt atattatttg acctttttta gtcataggcg aagttgtata atccgtaagc
    13921 atgaagaatc cacctaacat aagtcctcca gcaaacagtt gatatattgc gaaagttata
    13981 ttaaatccac ctaaaaggaa tgttaatata aatacagttg ctatgtaaaa tactggtata
    14041 acataattta ttatgccttt atacattagg tatactccac ctaataataa taatattgct
    14101 gatgtctcac ctatacatcc accaatattc cctatcatca tgtcagcaag actaattcca
    14161 ttgctagaaa ggtctgccag tcctgctgct ccatttttca aaaagcttaa tggagttgct
    14221 gtagtaactg catctaaatt tttacctatc cagttaacac ctggttgagt ccaagcagtc
    14281 attgctactg ggaatgatgc taataaaaat gctcttgcag caagtgctgg attgacaaag
    14341 ttacatccaa ttccaccaaa taccatttta actactaata ttgcaaatat tcctcctact
    14401 agacacatcc accaaggaag tgatgctggt acgttaaatg ctaataataa tcctgttaca
    14461 acagctgaaa aatctcctat agtacttttt tgcttcgtaa atttttgata taaaaattca
    14521 gtacctactg taccaagtat acaaaaaaac atagcactca aagcattaag tctaaagaag
    14581 tatactcctg ctactgctgc tggaaggagt gctataataa cttgtttcat tatatatgaa
    14641 gtatcttcat tacttctcac atgaggagaa gatgatacta tcaacttatt ttccatcgtt
    14701 tttccccctt aactttcttt atttctgagc agcttgcttt cttctctttg ctcctatttc
    14761 tctctttgct gatcttatag attgaagtaa tggtcttctt gctggacaaa taaatgaaca
    14821 agacccacat tctatacaat ccattgctct atgatattca gctgtgtcat agtcatcttt
    14881 taaagaatat gcacttatga aaagtggttg taagaatgat ggacatacat ctgtacatct
    14941 tccacatctt aaacagtttt gaatatctgg agtacgtgat tcttcctcat ccaagcataa
    15001 aattcctgaa gaacctttgt tagtcgctat ttcagtagta tattgagcta ttcccatcat
    15061 aggtccaccc attataacct taccaatctt cttatcttct ttaaatccac cacattgttc
    15121 tattatttct gaaactaatg ttccaacttt tgttattagg ttctttggct ctttgatgca
    15181 actacctgtt attgtagtga ttctttcaac caaaggcata cctgttttaa tagacttagc
    15241 tatttgtgct gctgtggcaa cattgtcaac aactgcacct gctgctattg gtaatccacc
    15301 agatggaact tctttgcctg tacacgcata aataagttgt ttctctgctc cttgtggata
    15361 tttcacttga aggccaacaa cttcgatttc actatagtct ttagctacat tttgaattgc
    15421 ttctatagca tctggtttat tcgtttcaat tcctataaat ccttttttga catctaatac
    15481 cttcattaat gctcttaaac caaacacaac atcttctgga ttttcaacca ttaatctgtg
    15541 gtctgcagtt aagtatggtt cacattctgc accatttaat ataactactt ctgccttgct
    15601 atctggtgga ggagatactt ttacatggtt agggaatgta gctccaccca tacctactat
    15661 tcctgcctct tttatgatac ctactatatc ttctttgctc aaatcctcta actgaccttt
    15721 tggttgaaca ctctcatgta attcttcctt aaagtcattt tcgataacta cacattgagc
    15781 tgaacctcct ggtacttcat gttgttcaat agcaataact ttacctgata ctgaagaatg
    15841 aacgttacat gaaacaaaac cttgttgttc acctattttt tgcccaaact ttacttcatc
    15901 accaacttca acaataggtt tagctggtgc ccctatatgt tgctgaagag gaatgtaaac
    15961 tatctttgga gcttgagctt tttcaagtgc ctttgtgtta gagtactctt ttctatatgg
    16021 aggatgtatc cctcccttaa aagttaagag tttcattgtt ttaccccctt atctataaaa
    16081 aaacattttt tctgccttct ataagtatac tatatatatt aaaaaatctt aacaattgtt
    16141 aaaaaaatat ccgtattttt ataatttatt ttataaatca atacctctat attaagtcaa
    16201 actgaggaaa aggattgctt aattaaacaa tccttttcat atagctttct aaagtccaaa
    16261 ttttctgtat tttattttat agaatttata aacatagagt actcctgcat ggatgataaa
    16321 agcagacttt tacttacact atatttttct tcatttgctg cttttgaagc tgtatcaatt
    16381 ttttcattca cattctttac atactctatt aaattatctt taaataaacg catttcagaa
    16441 tagtcaatct tttcagcttc ttttgatata ttttgtgata ttgcctttct atttgtcaaa
    16501 atagtattat atttttccat atctacatta ttttcagcat tcgaccaaaa acttgattct
    16561 tctttaaagt ttgttattaa tttatttaat tctttagata tactctctat ataagcgtag
    16621 ttacttgtat actctaaaga caacataggt gcatctaagt gtgataaaaa tgcatctgga
    16681 aacacctttc ttacactgct tatctgcttt ctaacatcat tttcatcaaa agaggaatag
    16741 gtctgtattt tttttactcc atccttctcc ataactgaaa aaggtatgtc attattaact
    16801 aatgatgtct gtattttttt caaatcatca ttattatcta ttgcagcaac ttgaatagtg
    16861 tacatatccc agccttctat agtagctaat ttgacttctt ctggctcttt agtgttattt
    16921 accgcttttt ctggattagt ttcttgttta tcatctgaat tggtttgtga tttagatttt
    16981 ttcccttttt ttattgaatt taattcatca gatatatctt tataagttat atctgatgaa
    17041 ttatttagaa atggtatttt agccgataca tactccaata tcttgctatc ttttattttt
    17101 gtataagaat aatagccaat tagacacaag caagcaatcg ttataacata tttacgtaaa
    17161 tcaatcttat gtctattatt attaaatcct ctgtatatcg tttttcttct attcattagt
    17221 ttgtccctcc caaaattata ttattaataa agtatatttt taagagtcac aatttatgaa
    17281 tataaataat tatttcttct aattttttaa taagaaattg tacttactaa acaaatcttc
    17341 ttgcaccaag taatacttta gaccaatatg aactagttaa attatccact ttaaccttat
    17401 taggattagt ctgagtacca ctacaatgga taaatttact atttcctaca tatataccta
    17461 catgattagt tgttccatca ccatcagtgt caaaataaac taaatctcct ggtgcataat
    17521 tccccataga aattgcactt ccaaaattag cctgttctct agaaactcta ggaatagaaa
    17581 cgcctacaga atgtttaaaa acatactgtg taaatccaga acagtcaaat ttatcaggtc
    17641 ctgaagctcc ccattgatat ggtattccaa taagagtata tgcaaaatct acgattgttg
    17701 aaccttttgc tgtagattta tctgaatttc cagtaaatga tggctttgag ttactattag
    17761 attgagaatt ttggttagat gatgaatttg gtttttcttt tgtaccttca ttagtctcag
    17821 aaatataact ggcactcacc catccaattg ttccactcga taactttacc ttataccaac
    17881 cattattttt ggattttaat tcaacaacat ctccaccatt taattttcct attatagaat
    17941 agcttgtccc aggaccactt cttacattta aactcacagc agatgttacc ttaccattct
    18001 tgccttcaat tgattcttcg ctagatggtt ttttatcaga attattagta ctatttgaat
    18061 tactagaatt attcgttcca acatcctctg atgttttaga gatatattgg ctactagccc
    18121 atccagtaac tccatttgat agttttattt tataccatcc attgctttgt tctagtaact
    18181 ttactacatc tccattattt gctttaccaa ctaatgaata gttagtacct gcaccacttc
    18241 ttacattcag tctagaactc acatttactt taccatttcc actaatagtt gtcccacttg
    18301 aagttggatt attttgattt gatgtacctt cagatgaacc acttgttgat atgtattttg
    18361 cgcttcccca tccaacaaca gaactcgata cccttacttt ataccatcca ttagactttt
    18421 ctaaaatctc tacagttttt cccttgtata attttgctac cttatcacaa tcagaacttg
    18481 gtccacttct tatattcaag gcacttgctg ttactgttcc cttttcaaga gcacttgcat
    18541 ttatggagct aacagatact gctactgccc caattcctaa agctgctata gctttcttca
    18601 ctactatcac tccccccaac aaatttttat agtcataaat tcataacatt attatccatt
    18661 tttagtccct acaatttaat gataacttat tctcttatta attgtaaaga aattataaac
    18721 atatccttaa aattaatgat ttttaggaag tatttcaatc caataattat ctgggtccgc
    18781 tataaaataa attcccatac tttcattttc aaaacatatg caatctaaat ctttatgaag
    18841 tttttttgcc tcatcaaact cctcaacttc taaagccaga tgaaattcat tgtctcctaa
    18901 attatatggt ctgtcccaat ctctaagcca agttaattct aatgtatgac tagtaatacc
    18961 atctcctaaa taaactaata taaaacttcc atcttctgct tccttacgct ttacctcttt
    19021 aagccctaaa gcttttttat aaaaatctaa acttttttct aagttagtaa cattgaaatt
    19081 gttatggcaa aaattaaact tcattttgaa atcactctcc aatttttatt ttttttatat
    19141 tatatcatta ttataaattt atttaaatca tattaagtta aaaatttgaa tgatgatgac
    19201 caattaccaa atggtggtaa attatttaaa agaatatgtc aaaaaaagaa taagctcttt
    19261 caaatgcaca aaaaatttat acccaagtta tacttaatca tgcaaattgg cagaacttat
    19321 tctttaaaac tctaactttt aagtttttct ttgataatat aatttattac tttttttcta
    19381 atatcttcag cattataatc atcttctact tctatatcca aaagttttgc tattgacctc
    19441 atatcttctc tagtctttga cctatagctt gatattaatt catcttttat tttctctggt
    19501 tctaaaagat attcttcatc tcctcttttg aaaacaacgt atttttgagc atatgaactt
    19561 ctattatagt atatatatct tgatattttt cttaaaattt gattatatga aagtcgcttg
    19621 ttaatatcaa gttttaagta ctctgaaaaa gcaaatagct ctttttttga tttaaataat
    19681 gcttctttct ttatagtcct tgatattgaa gttacacttt cttgatatgc acactttttt
    19741 aacattttta aatatactct attaagacca atactttgat tgaatttttt tatattatta
    19801 ttaataatct tatccaagtt accttcaaag tatttttttg ctcctttttg tgatacataa
    19861 ctctcaagtt ctttgtgggc catatcaagt atcatttttt ttaaatcctc tttatatccc
    19921 ctagattcca tgatactccc tcccttagct atttatagta tattcaaaaa gctcaagaaa
    19981 tgtgagtcat tacaaaattt ttttactttt tatgtatata ttaatttaaa aggggagata
    20041 atagtaatgt aaaatcaaac atactcaatc tcccccattt ttagacctct attactcccc
    20101 caatagaggc ctttttttag tttatcaatt tctattcaat tccctttagt tcaaatccta
    20161 attctgctat cacttctttt aatttttcaa tagatatatc atctttcata tctacagatg
    20221 cacacttatt ttctaaatcc acatctaaaa cttcaatacc atctatatct tctgttaatg
    20281 cagttttaag atggtttaca caatgaccac aactcatacc ttctactaat agtttcttct
    20341 tcatttaatt acctccttat accccataag ggtattctat catatagttt atacttttta
    20401 aatagaaatt aatcatttaa tttgattcat tcgattttat atctaaatta gatattccct
    20461 catatacatc aaaatcattt ggagtaattt taccttttaa tctactatta cttacaatat
    20521 agttgtttct cacagctaaa cttataaatg ttgcattatc ttttatatat gtctgtacat
    20581 ctttgtatgc tttttttgtt tcaatttcag acgtagaact tgtaagtgca gacatatatc
    20641 catttagctt atcatcagta tatccactac ttgctataat actacttgca tctggtataa
    20701 ttgatagttt ccatccaact aatgctaaat cataattttt agaattaaga gcattttcca
    20761 tatccttatc tgacagctga tttactgtac tatttattcc tatagctttt aagttttcat
    20821 ttattatgtt tgcagttttt attctctcac tattatctct gtttactatt atctttaagt
    20881 tcatttcaga aagacttttc tttacttcct ctttttctct attttttttc ttttcagctt
    20941 cttttttgat tctatcttct tctgcttttt tagcttttgc ttcttcttct ggcgtaagct
    21001 tctttgagtt ctttttagct gattttttct gattttttac tttattgtca ttttgattta
    21061 ctgagtttat atttgcatat tctaccttag caagacactc tatcgcctta tctttattaa
    21121 actctaaatc cttcatttca ctattatagt attttgattt tgaattaagt ggaaaattaa
    21181 ctggagtagc atcatccata taaccttcac taataattct atctttatct atagagtgag
    21241 caattgcttt tctaaaatta acatctttaa aaaatggatt attataatta aataatacac
    21301 actcataatc tcttccttga tattttgtta tattaaactc tttttcttga aacttgctta
    21361 aatcatttaa tgtaacattg gttatatcac tatcaagagc cataaccata gatgtccttg
    21421 cctcttcatt aggcaccata cctactttta tgttcttcat ggtctttgga acttcttcat
    21481 agtaatcttt gtttacagat aaaaccattc cttctctttc tgtatatgat tctattttat
    21541 atttcccatt tcctatcata ttattattct tgtcatttac atcctttgaa tctaaatgat
    21601 tttgagatac gattggaaaa attaaactat caattgaaaa agcatatcta gctttaaatt
    21661 ttattgtaaa atctttatct gatttaatat tcacagaagc tatattttta gtaaattcat
    21721 tatatggact atctgcactt ttttgaatta aaccaatagt atagtttaca tcatttgaag
    21781 taacattttt tccatcatgc cactttgcat cttttagctt tatatctata ctcattccat
    21841 cctgagctat attatactcc ttaactaatt gaggaattac attgtagttc tcatctatag
    21901 taaataaact atcataaact aaattcatta tataaccaac agacttatca gtattagtta
    21961 taggatttat agtttttgga gttaccattg ttaagtttat atacttagca tctatagaac
    22021 tttgactttg taaacttgaa tcactactac tttgttttgt tttagtatta ctacaaccag
    22081 ctaccataag agttatagct aaaacgactg ttaagacttt tattctcttc attagttttt
    22141 tcctttcttg ccatatatct tattataatg ttcgtaatta ttttttactt tttctacata
    22201 ttttgaagtt tgcttaaaag gtatatcatg tagattttcg ccatctttac tatattcatt
    22261 attttctaac cattttttca cattacctga accaccatta tatgcagcta tcactaaatc
    22321 taatttacca aattctttgt ataacttact taaataccaa cagccaagtc ttatattagt
    22381 ctctgggtcg aaaatatcca cattttttaa atttagttcc tctgctcccc aatctctagt
    22441 tatatctaat atttgcatta atccttttgc ttcctttttt gaaacagcag aactattaaa
    22501 cttactttcg gctttaataa cactgtaaac tatattttca tctaaattaa attcttttga
    22561 atacttttct acatactctg aatatttttt aggatataaa aatttatgta ttactttgct
    22621 ttccattaat agtgccccaa ataagattat aaaaatagat agaatcaata ctttcttact
    22681 attcacgcca attctccttc atgtactcaa taaatttatg tgctttactt tctaattcag
    22741 ttattgttcc cgaattatct attatataat caccatattt ggatttttct tcctgactca
    22801 tctgtgaatt tatcctacta agtgcttcct gcttactaca attatctctc ttttgtattc
    22861 tactaatttg tacttcttgt ttgcaagtaa ctactaatag cttgtcaacc atatctaaaa
    22921 aaccactttc taccaatatt gctgcatcta atactactat attttcgcct ttttttctca
    22981 attcttctat ttcacttatt attttttctc ttattcgcgg atgagtcaag ctatttaatt
    23041 ccttaagttt ttcttcatcg ctaaaaacta ttttcccaag agcttttcta tttagagttc
    23101 catcatcatt ttttatactt tgcccaaaat ggacaaatac tttttccaat aacaacttat
    23161 cctcaaatat cttcctggat ataatatcag catccactat agaaatatta agatttctaa
    23221 atatatttga taggctgctt tttccacagc ctattcctcc tgtaagtcct aatatcaaca
    23281 taaaatcacc tactttgtct cataccaaga actacctaca tttaaatcta catctaagtg
    23341 aacatccata tttacagcat tttccatttc ttcttttact attttcttaa ctatatctat
    23401 ttcatcatca attgcttcaa ctatcaactc gtcgtgaact tgtagtataa gttttgattt
    23461 caaattgttc tcttccaatt ttttatgaac atttatcata gcaattttta ttatatctgc
    23521 tgcacttcct tgtattggtg cattcattgc aaatctcttt cctctatttc tttccataaa
    23581 attgcttgac tttatttctg gtatatatct tcttctgttt aaaattgtta cagcatatcc
    23641 atcctcacta gccttttcta ctgttgtgtc cataaactct tttattttat ggtatttagc
    23701 aaaataactt tcgatatatt ctttcgcctt tggaacaggt atatttaaat catcagctag
    23761 accaaagtca cttttaccat atataatacc aaagtttaca gctttagctg cacctctcaa
    23821 ttcactagta acatcttcca taggaacatt aaaaacttgt gaagctgttt tggtatgaat
    23881 atcttcatga tgtttaaatg cttctatcat attttcatcc tgactcatat gtgcaagaac
    23941 tcttagctct acttgtgagt aatcggcatc aaccaactta cagttatcat ctgcaataaa
    24001 cacttttctt atttttctac ccatttccat ttttataggt atattttgaa gatttggttc
    24061 tgtagatgaa attcttcctg tagtcgttat agtttgatta aatgatgaat gtattctatt
    24121 actttctgga tttattatat ttaaaagtcc ttcaacgtaa gtagaattta actttactat
    24181 ttgtctatac tcagttatct tatctattat tggatgctta tctcttaatt tttctaaaac
    24241 atctgcatta gttgaatatc cagttttagt ttttttaatt attggaagct ctaatttttc
    24301 aaataatata acacccaatt gttttggtga atttatattg aatggctctc ctgataattc
    24361 ataaatttcc ttttctagtg tagctattat ttctttaaac tctacactca attcatccaa
    24421 tatacttttg tctactttta taccttcata ctccatatga cctaagactt ctactaaagg
    24481 catctctaca tgataaaaaa gaccatccat actcatatcc attagacttc tttccatcat
    24541 aggaattgtt tctttaactg tgcatattat ttgacccata tactctgcta aatcctcaaa
    24601 ttctaaatcc tcatagtttt tagatttaac accctttcct aataattctt cttttgattt
    24661 aacctttttt gataaatact tcatagctat tgcactacaa tcatatgagg tagatgaagt
    24721 tgaatctatt aagtactcag ctattgttat atcaaaatac atattttcta agtttatatt
    24781 atatggtttt aaagatatat aatcatcttt taaattatat ccatactttt taatttcatt
    24841 atttgaaaat atactatcta actttgacac ttcatttgaa tctacataat atattttttc
    24901 tccatctaca cttataaaaa tatatattat atttttttca agtatattac cttgtcctct
    24961 aacagttttt aaaataactt gtcctttttt gtttacttca tctataaatt tttctgtatc
    25021 ttttaatttt aatatttcta ttttttcttc tacgtgttct gtgtcatctg aatcgaccaa
    25081 atcaactaat cttgataaga gacttgtaaa tccaaagtat ctaaatttat ctaaaagttc
    25141 atctttatca taatcgccaa aaaccatact atctaaatcc acttctattg gcacatctct
    25201 tataattgtt gcaagtcttt tactttgaat agctatttct ttattttcct ctattttctt
    25261 ttttacacta cctttaagct tatctgtatt ctctattaaa ttctctatac tagaaaactc
    25321 ttttataagc tttatacctg ttttttcacc gattccaggt actccaggta tattatctga
    25381 cttatctccc ataagacctt ttaaatcaat aaattgtgct ggagtcatct cgtatttttc
    25441 aattacagaa ttaaagttgt attcttcaac ttctccaact cctttttttg ttataagagt
    25501 agttgtatta tctgaagcaa gttgaatagc atctttgtct ccagttacta tgtatacctt
    25561 atacccatta ttttctgctt ttttagaaac agttcctata atgtcgtctg cctcataccc
    25621 atctatttcc aatctattta ttttgaattt atctaaaagg tcttttaatg gctgtaattg
    25681 ttctgccagt tcatctggca ttccctttct tccagcctta tactctttat attctaaatg
    25741 tctaaatgta ggtgcttttc tgtcaaaggc tacacttata tgtgttggtt tatatatatc
    25801 tatcatttta aatagcattg ttgtaaatcc atatattgca ttggtcttta acccttcttt
    25861 attattcatt tctggcagag cataaaaggc tctgtttatt attgaatttc catctattat
    25921 aattaatttt ttttccaaat tatatcactc ctaaaaatta tctatttttg taacatatat
    25981 attatatacc aatatagtgt tatttaatat tcccataata ataatagtat attaaaatta
    26041 aatatgttgc aaacaatata tttttttgct ataataatat acatgacatg cccgaatggt
    26101 tgaattggta aatacaatgg attcaaaatc aactgtgttg attcataagt ttttataatg
    26161 ttttacagtt tgatgagtat tttgtattca atattcctct tgcatattct tggaaaatct
    26221 ggtaggatat acaagtagtt tggatttaaa atataagccg ctgtggcgga actggcatac
    26281 gcatacgact caaaatcgta cgggaaacca tatgggttcg agtcccatca gcggcaccaa
    26341 taaaaaaaac tctttagtat atactaaaga gtttttttat tgtaatattc tttcatcttt
    26401 atttctagcc ttgtataagt ataaatacca tagtcataac ttcttgaact cctaaaatat
    26461 atctaaacaa agtaggattt tttatgtatt cacttaataa atccatactt cttatcatat
    26521 tgattttttc ccaatctcta aaaattttga tacaaataaa aaatacagag aactacttga
    26581 tatactagaa ggttattctg taccatttgg tattgctgta ggtgttccag atggaaattt
    26641 accaaatgct agagatgtag tttataaagt ttctagagtt taggataaag tgttgcaaca
    26701 atcaattagt tacttgaaga gttaaatcag taatatacaa ataaattaat ggaagaggaa
    26761 gctatttaaa aagtagttta gatttttcat tgtaaaataa ttaaataaag aataaggtga
    26821 taagtatttt atttattatc ttattctttt ttatttgtgg aatgataaaa ataagaatta
    26881 agtgtaatac aatgtaaata aaatgtgtta attttaagta tattttgtaa agtttataaa
    26941 atctgaaaag ctcaagatat attttcacta taatggataa aatatcaaaa tattgaaata
    27001 aacaaaaaat aatgatataa taagaaaaaa taaagaaaaa aagtaaccct tagttgtaaa
    27061 agggttattt ttttgtgata aaatatccat aaaaatattc ataccaaagt aaattaagat
    27121 gtatttcatt tctcaaaaat atcctaatat aaaaaataaa gaaatattta ta
//
LOCUS       DAELSY010000040        27110 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.040, whole genome shotgun sequence.
ACCESSION   DAELSY010000040 DAELSY010000000
VERSION     DAELSY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27110
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.040"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            600..1256
                     /locus_tag="KPG09_001520"
     CDS             600..1256
                     /locus_tag="KPG09_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966138.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(1726..2997)
                     /gene="sleC"
                     /locus_tag="KPG09_001521"
     CDS             complement(1726..2997)
                     /gene="sleC"
                     /locus_tag="KPG09_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBG0966139.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIVAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGADTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            3242..3958
                     /locus_tag="KPG09_001522"
     CDS             3242..3958
                     /locus_tag="KPG09_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBG0966140.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            4037..5347
                     /locus_tag="KPG09_001523"
     CDS             4037..5347
                     /locus_tag="KPG09_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBG0966141.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            5447..5983
                     /gene="lepB"
                     /locus_tag="KPG09_001524"
     CDS             5447..5983
                     /gene="lepB"
                     /locus_tag="KPG09_001524"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG0966142.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            6149..6685
                     /gene="lepB"
                     /locus_tag="KPG09_001525"
     CDS             6149..6685
                     /gene="lepB"
                     /locus_tag="KPG09_001525"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG0966143.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            6695..7450
                     /locus_tag="KPG09_001526"
     CDS             6695..7450
                     /locus_tag="KPG09_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBG0966144.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDIRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVREG"
     gene            7524..9191
                     /locus_tag="KPG09_001527"
     CDS             7524..9191
                     /locus_tag="KPG09_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBG0966145.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVILIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            9764..10693
                     /gene="glsA"
                     /locus_tag="KPG09_001528"
     CDS             9764..10693
                     /gene="glsA"
                     /locus_tag="KPG09_001528"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBG0966146.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            10791..11354
                     /locus_tag="KPG09_001529"
     CDS             10791..11354
                     /locus_tag="KPG09_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0966147.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            11842..12678
                     /locus_tag="KPG09_001530"
     CDS             11842..12678
                     /locus_tag="KPG09_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBG0966148.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHIGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            12930..13925
                     /locus_tag="KPG09_001531"
     CDS             12930..13925
                     /locus_tag="KPG09_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG0966149.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRNKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(14182..14586)
                     /locus_tag="KPG09_001532"
     CDS             complement(14182..14586)
                     /locus_tag="KPG09_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG0966150.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     FLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            15586..16500
                     /gene="queG"
                     /locus_tag="KPG09_001533"
     CDS             15586..16500
                     /gene="queG"
                     /locus_tag="KPG09_001533"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBG0966151.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFVDNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEMPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            16606..18843
                     /locus_tag="KPG09_001534"
     CDS             16606..18843
                     /locus_tag="KPG09_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0966152.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNRLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDKSDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNESSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            19040..20092
                     /gene="nth"
                     /locus_tag="KPG09_001535"
     CDS             19040..20092
                     /gene="nth"
                     /locus_tag="KPG09_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBG0966153.1"
                     /translation="MKNVNLKEINEKDVIVEEDINLKGMDKKDVIEKEEVNKKSIDKK
                     DIIEKEEIDKKNVKKVEVQKEIDKMISKKDEIIDEKDIENFRYIEEDKKSIKVSKKST
                     KKGETEKKTKKSSSNKATQKQSKEVKSKSKIKSESANKKDVNKILDELEKLYPDAKCE
                     LNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGL
                     YKSKSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDT
                     HVFRVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASC
                     PIKEDCNYYKELNETK"
     gene            20286..20756
                     /gene="trmL"
                     /locus_tag="KPG09_001536"
     CDS             20286..20756
                     /gene="trmL"
                     /locus_tag="KPG09_001536"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBG0966154.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            20792..21640
                     /locus_tag="KPG09_001537"
     CDS             20792..21640
                     /locus_tag="KPG09_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG0966155.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVDEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            21979..22983
                     /locus_tag="KPG09_001538"
     CDS             21979..22983
                     /locus_tag="KPG09_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0966156.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNAALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGIKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            23108..24220
                     /locus_tag="KPG09_001539"
     CDS             23108..24220
                     /locus_tag="KPG09_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0966157.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSVPTSAEKDKLNSKSKNEEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            24643..25404
                     /locus_tag="KPG09_001540"
     CDS             24643..25404
                     /locus_tag="KPG09_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966158.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            25407..26225
                     /locus_tag="KPG09_001541"
     CDS             25407..26225
                     /locus_tag="KPG09_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBG0966159.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            26405..27007
                     /locus_tag="KPG09_001542"
     CDS             26405..27007
                     /locus_tag="KPG09_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBG0966160.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
BASE COUNT        10897 a         2564 c         5033 g         8616 t
ORIGIN      
        1 tgtagttata aagcatattg ggaataatga aaaaggttaa aaacatatta ctataaagta
       61 tagggggtag gttaaaagag taatcataaa ataaatatac ggataatagt agataagaat
      121 accagaaaaa aatagatagt taaagggtta gggtgaaaga gtaatcgtaa aataaatata
      181 tagataatgg tagataagaa tatcagaaaa aatagataat taaagggtta gggtgaaaga
      241 gtaatcgtaa aataaatata tagataatgg tagataagaa tatcagaaaa aatagataat
      301 taaagggtta gggtgaaaga gtaatcgtaa aataaatata tggatgatgg tagataagaa
      361 tatcagaaaa aaatagataa ttaaagggtt agggtgaaag agtaatcgta aaataaatat
      421 atagataatg gtggatagag tagtaagaaa ataaatagat aaagggatag aataaattta
      481 aatatggaaa atgaatatct aataataagt gattaataaa taattgtaga tagattgtaa
      541 attaaataaa tctttaatag aaaggagaac tatttatata ttaaatagta tttataaata
      601 tgaaaaaagg cgattttata tggagtggag ttcttatttg tattattgct gtcttagcaa
      661 taccaagtag tagagaagta tttatggaag caacaggtgc acatccatat attgggggtt
      721 ttttaaagtt tgctgtatta gctacaatgg gagatttact tggtgctaga attttgtata
      781 aggattggaa aataccagta ggagtatttt atagagcaat agtttgggga ataataggtg
      841 tagttaccac atatggaatg tcaatctact catcaggtgt aggtcaagca caagtggatg
      901 gtatgcttcc atttgaggga atagcttttg caaatgctct atttaagagt gcaactatga
      961 atatactaat gtcaccaact gtatttttat tccataaggt tatggactgt tttatagata
     1021 aaaaatatga ggttggaaag gggattaagg taaaacttaa agatgtggta aatattgtgg
     1081 actggaatgc ttaccttggt tttagtgttt taaaaactat accatttttc tggataccat
     1141 gtcatacaat tgtattctta ttccctgctg agtatagagt aattgcttct gcatttgcat
     1201 ctatagcttt aggtctaata ttggctttag ctaataagtc taaagttgca gtttaggttc
     1261 actgatgaag ataatagtat tatttaaatt atgaataact ttttaaatta atattattta
     1321 tatttataat aaataataat ttagataaga aggaatattc taagtgatag atagtgtttt
     1381 aaataataag gagtaatatt ctaagtgaga agtagcattt taaataataa gtgagatttt
     1441 aaataaaaag taagcttttc gttagaatag cattttatta aacttaaaaa taaatataat
     1501 attttaaaga aatctaagtt gctaagcact gtttttaaga agcttaaaat cccccattta
     1561 aagttttaag attctaaaaa aacaagttat aactaaaaaa tggctgttaa gactacaata
     1621 cttataagta aaaatactta tggtatatat tagttcataa cagccatttt tattttaaat
     1681 taataatttt attcaataat ttgagtttat gttctaaatc aagctttaaa ttaaaggatt
     1741 taaagaagct attctagttg tagctacata aactctggat atttcatacc atgttttaaa
     1801 atctactact ccgctttgag gtaaaccaaa gattccttgg aatatcttaa cagcgttttc
     1861 tgtatctgct ccatagatac catcttctat aacttttggt acagctggat aagaattaga
     1921 tatagcattt aactgatttt gtatggttct aacatactgc ccagaagaac caacttgtag
     1981 tgtagtacca gggaatgaca ctggtacacc acttactata ggtgctcttt caaatactat
     2041 ctcatctcca tagaaatatc ttaggatact ctcatagtca tatccttggt cacctaagtc
     2101 tttactgccc cattgagtca tttggtcagg acattgagat ttttgtccat cacaatattg
     2161 tgctaatagt ggctgtcttg atgtaggagg tctttttata aaagtattaa aaatagcatc
     2221 tacaactacg ttaattggtt caaataagtt tctattattt ataaacttat ggtcgtatgc
     2281 tgttgttgaa gttattgtaa agttatatcc tttatttcta taccattcag taaagactct
     2341 atttaaagta aatgatatta tagctattac atttgcatag attgtctgtt caggccaagt
     2401 tgaatatatt tcagatgacg ctatgttttt aatgtactct ttaaatggaa tccaatagtt
     2461 tggagctgat gaatcttctg gcaaaccatc atgaactact ataaattcag gcactacagg
     2521 gttatctaat acaacgaaac cagtaggagg tggtaatggt tttaagttac tttctggaat
     2581 cttaggagga taagtaccat atagagtatg ttctcctatg tcaaaaatca actcactttg
     2641 tcttgagaaa gaacgcttac ttcttgttct aggagacatg ggaactcctt gtcttgctac
     2701 aattgtagct aacaactgcg ttccttctat gacaactgtc tcatatccat ctgctatggc
     2761 ttccactata tactggctat aaggtctaac atcagatggc tgtagagagt aatctacatc
     2821 tggtgctggt aatactaaac cagtcacttg accagattca ttacttctta ggttttggta
     2881 taaagttgat gagctttggc taccattaga cattgaatat atgtttacta ctgcattttg
     2941 aataggtcta ttgttagtag catcaattat gcttactgtt aagaaaccat cttgcatcaa
     3001 atcacccttt ctttaaatga atttagttcc cttatatttc aagatatgaa tatagcttga
     3061 aatttgttaa ataatataga tataaattta aatttttttg agtatttgag tatatttgta
     3121 tttaaataat ttaaaataat ttaatgtata ttttagtatg tatttaaaac acaaataagc
     3181 taaaaataag tagatgatat aaatcataaa taaatcaaaa attttgaaag gaaaataatc
     3241 tatgaagcta gatttatttt tattagcaat agttcctata ttgataggga tgttttggat
     3301 aaggtctaaa gatagatatt gtagagaacc cctaatccat ctaattaaat tttttttaat
     3361 aggtgctttt ctaagtgtta taattatact tttagaaaat ttactgatga agtttaatgt
     3421 atttgaaggg tattcagaac taatatatgt atcatttgta gttgcaggac ttgtggaaga
     3481 aggtgtaaaa gctctgatac tgattcctgc cctaattaaa gaaaaacact ttactgaaaa
     3541 gttagatggt attatttact ctgtattttt agctttagga tttgcaacaa ttgaaaatat
     3601 ggtttatatt ttttctgaaa gtagaaatct agcactgcaa gttggtatta atagggcagt
     3661 tatctctata ccagctcatg taatgtttgc aattactatg ggctactata tttctaagta
     3721 taaatttgag ggaaataaaa ataaaagacg tgagtatttg tttatggcag tgttaattcc
     3781 aattttatta catggagttt ttgatttcat attgatgata gaatatagat gggctattat
     3841 attacttata gtttatgtaa taattttgtg gaagataaat cttgataaac ttgagaaata
     3901 tatgaatcac tctaaaaaag tgtttttcgg taatcttagg aaaaagaaga agaaataaaa
     3961 tttaaaggaa agacataaat aattaaagta taataaatga cacacaagta aagcagattg
     4021 aagggggatt atagatttga cagaattacc aaaggaattt ttagaagata ttgaagaaat
     4081 actaaaagat gaatatgatg attttataaa aagttatgag gaaagtaaga ctactgggct
     4141 tagaataaat actttaaaaa ttgacaagaa aaaattattt gatttggagt tgtttgattt
     4201 gaatcaaata ccatgggcag aagaaggttt ttattatgat gataaaattg acagacctgg
     4261 tagaaatcca ctgcatgagg ctggagcata ctatttgcaa gagccttctg caatgagtgt
     4321 aataccaaag gcagatatta aagagggtga aaaagttctc gatatgtgtg ctgctcctgg
     4381 tggaaaatct acatatatac tatctaagct aaacgataca ggtttattgg tttctaatga
     4441 aattaatgct agtagaatta aggctctagg ggaaaattta gagcgatttg gagcgagaaa
     4501 ttgtattata acaaacaccg attctaatag cttaagaaag acgtttaatg ggtattttga
     4561 taaggttatt atagatgcac cttgttctgg gcaaggtatg tttaggaagg atgaggttgc
     4621 catatcagat tggagttatg caaaggtagt agaatgtcaa tccatccaaa aagagattat
     4681 cagagatgga tataaaatgt taaagaagga tggcatttta atttactcaa cttgtacttt
     4741 ttcaagggag gaaaatgagc atgttataaa tgagtttatc agtgaatatc cacatgcaga
     4801 acttattgaa atggaaagat tatggcctca taagataaag ggagaaggtc attttgttgc
     4861 caaaataaaa aaattagaag aagaagaatg taatgtcaaa gaattaaaaa taaaaaattt
     4921 agataaagaa ataaaggatt atagggagtt tgaaaagaaa tttttaaagg ttgattttta
     4981 tagtaataaa aaaaataaat tttatttaag aggagaaaat ttgtacctac ttcctgaaat
     5041 atatccagat acgaaaaaac taaaagtatt gagatacggt ttgcatttag gggttttaaa
     5101 gaagaataga tttgagcctt ctcatgcact atctcattac ttaaaagttg aagatgtaaa
     5161 aaatgttgaa aacttcagtg tacagagtga atcaattctt aaatatttaa aaggggatgt
     5221 tgtgaattct aatgatagta gaggatgggt tttggtttcg gttgaaggaa tacctttagg
     5281 ttggggaaaa gaatcttctg gtgtaattaa gaatcattat cctaaaggtc ttagaaaagt
     5341 attttaaact attagaaaaa agaagtataa tatttatatt gtaggtttat ggtagattat
     5401 tttgctgtag attgacttaa tatttaacac agaggagacg attttagtgg gtgaagcagt
     5461 taaaaaagaa gttgtagaat ggataaaagt gattgtcata gctcttgttt tggcatttgc
     5521 aataactcgt tttatagtgc caacaatagt caaaggagaa tcaatgtatc ctacattagt
     5581 tgaacgtgat tatttgatag ttaacagaat tgcgtacaag gtaggagagc caaaatacaa
     5641 agatataata gtatttaaaa ccgacttaac agaggaaaat ggaaagaaaa aagatttagt
     5701 aaaaagagtt atcggggttc ctggtgacca tgtaaaaata caagactcca aggtatatgt
     5761 aaatgataag ttgttagatg agacttccta tatacataat aatcgtactg atggagatat
     5821 tgatatcgta gttccagaag gaaaattatt tgcaatggga gataatagag aaaaaagttt
     5881 agatagtaga tacgatgagg ttggattggt cgacgagcat accattttag gaaaggttct
     5941 agtcagattg tatccatttt ctaagatagg aactattgac taaacatata aaatttggag
     6001 caatcgctga ttagctgaaa tcaccagacc atacacaata gttgtctata taaaaaagac
     6061 aacaccttat ttagtagact tatctaaaag atagtgttta agagacatta tcaataaaga
     6121 taatataact ataaaggaga atttgtgaat gaatgaaact attaaagaag agattgtaga
     6181 gtggataaaa ataattatta ctgcactttt ttttgcattt attataactc gttttataaa
     6241 accaacacta gtaaatggag aatcaatgta cccgacactt aaatcacatg attatttggt
     6301 agcaaatagg atgacatata agttatcaga accaaaatgt ggagatataa tgatatttaa
     6361 gactgattta ttacaagaga atggaaggaa aaaagagctt gtaaaaaggg ttataggtgt
     6421 tcctggtgac catctaaaaa ttaaggattc taaggtttat ataaatggta agttattaaa
     6481 tgaagtttca tatatacatg ataattatac tgaaggcgat attgatatgg tgattcctaa
     6541 gggaaaagta tttgcgatgg gagacaatag agaagttagt ttagacagta gatataaaga
     6601 agtgggattg gtagatgaag aaaatattaa aggaaaagtt attttaagag tatttccttt
     6661 tacagatata ggtatttttg agtaggtgat ttgtatggca gttggtatag cgtccttatt
     6721 gtttaatatt gaggaggctt taaacatttg tgagagtata aagcaaataa ctcatttaga
     6781 gattggaatt gacaatatat ctgaatgtag cgagttatgt aaatataaag agagaatttc
     6841 taagttagga ttatctatag ggatacactt gcctatggaa ttaaatactt gcgaaaacat
     6901 agagtatata cgaaattcat ggataaattt tattgagaag atagaatttg aactaaaagg
     6961 atttgatata aggtatttta accttcattt gggatatgtt atgacaaata gagttagaaa
     7021 gaatagagac aaatatttag gaaatagtgt ggatttttta gataaattaa atacaaattc
     7081 atatgtatgt atagagaata cttattcaaa aggtggggat ttttcaaata ttggaaatat
     7141 atcttatgat tttgaatata tatttaagag gattaaaaat tctaagattt gtttttgtta
     7201 cgatactgga cactgtttaa ttgatgagga tgcttatgta aagaacttaa aggataaaat
     7261 aagactgata cacttaagtg ataatgatgg tatcaatgat acacatgttg gtataggaag
     7321 aggaatttta tctgaagagg ggattaaaga ggtcttaaca ttagatgcag aatatttagt
     7381 attagaaata aattacgaag acattgaaga tactataagt aaattgaata atattgtaag
     7441 agagggttaa atacctctct ttatcttgtt gatatgcctc attaactaaa atttatattc
     7501 atcaaaagga aggagatgtt gaagtgaaaa aaataaactt aaaaatagaa ggaaaagata
     7561 aggaatattc attagaagaa aattcccctg gaatcagatt gggagatata gcgaaagaat
     7621 tttgtgatga gcataagggt tatataacac ttgcagttgt agacaataaa ctaaaagagc
     7681 taaattgtag agttaagaag gattgtgaga taaatttttt agacactact aatgaagatg
     7741 gagagagagt ttactttaga gtcatgtcat ttatatttgt aatggcttgt agagaaatct
     7801 tttgggatag cagagttaca atagaacatt cactatcaga tgggctatat tgtgaagttc
     7861 atatagatag aaagcttaaa gaagcagatg ttgaaaatat aaaaaacaag atgaaagaga
     7921 tagtaaacaa cgattatgtt atagaaaaaa ttgaagttac aagaaatgaa gctatttcta
     7981 tatttgaaaa taatgaaatg tatgaaaagg cagagctttt gaaatacaaa gagtatgata
     8041 gtgctaaagt atataagtgt agaaactata tagaccattt ttatggttat atgttgccat
     8101 caactggtta tataaaatca tttgatatta agctttataa tggaggcgtt ataattttag
     8161 ggcctagtga agaagataag actcttccta tgaagtttgt acctcaacct aagctatcaa
     8221 gtgtatatta tgaagcagaa gaatggtcaa gacttatggg aattgacaag gtagtatctc
     8281 ttaacaaaat aatagaaaac aatgaatatg gagatataat aaggacattc gaagcattgc
     8341 atgaaaagaa gttatcccaa atagcggata tgataaagga tggcaataag agagtaattt
     8401 taatagcagc tccatcttca tctggaaaaa caagttttgc tcacagatta tcaatacatc
     8461 ttagagtaaa taatttaaat cctatttcaa tttctttaga tgattatttt atagatagaa
     8521 aacatactcc tttagatgaa tatggaaatt atgattttga atcaatttat gcaatagatt
     8581 tagagaagtt taacttagac ttaaagaaac ttttagctgg tgaagaaatt gatgatataa
     8641 gattcaattt taaagaaggt aagagagaat acactggtaa aaaaataaag atagattcaa
     8701 atcagccaat tatattagaa ggtatacatg ggctaaatcc tattttaaca tcatctatac
     8761 cagatgaaga taaatttaag atatacataa gtgcccttac tcaaataaat ttggatgacc
     8821 ataatcgaat ccctactgct gatttgagaa tgataagaag aattgttaga gactataatt
     8881 ttagaggata tagtgctgat aatactattt tacaatgggc atcagttaga agaggagaaa
     8941 agaagaatat attcccgtac caagaggagg cagatgctat atttaactct gcttgtgtat
     9001 atgaattagc agttttaaaa cgatttgcga aacctttgtt ggaagagata aaggaagata
     9061 atcctgcaca tattgaagct actagattac taaagttttt gcagtatttt gttgaattgg
     9121 acgatacttc tgacatacca ggtatttcaa ttcttaggga atttattgga ggaagtaaaa
     9181 tagttgatta atatgtgtat tatagattgt tttatgaaga ttaaatggca tagcaagtag
     9241 tgtgattggt ttatgttaag tatattaagt gtaaagtgga gaattaaatt gatacagatt
     9301 ttaaagtttg agttgatata taaaaaggct tgttttatac ttttgtatga agcaagcttt
     9361 ttattttaaa ttttactttt ttgtttaata ttttgttagt ttatgtgata attttttaaa
     9421 agagtaaaag agtaaaagag taaaagagta aaagagtaaa agagtaaaag agtaaaagag
     9481 taaaagagta aaagagtaaa agagtaaaag agtaaaagag taaaagagta aaagatgtta
     9541 ggttagaaat ttttgacgtt ataagaattt aaattactgg attattagat tactaaatta
     9601 ctagattatt aaattattaa gctatttaaa ctgtttaaat tgtttgaaaa atgaattgat
     9661 tcttgtagaa aatagtttta aagtataatt ttttttaaca ctgttttatt atagtacaaa
     9721 cttgtgctaa aatagaatat ataacataat ataagaggtg catatgaata cgttagatga
     9781 gacattatta aaagaaatta taagttctaa taagaagtat actaattatg ggcaagtcgc
     9841 tagttacata ccagaattaa aaaacgctag aagaaatgat ttaggaattt gtatcataga
     9901 tagcgaaaat aatctttata gtgctggaaa ctgtagcaca aagtttacta tacaaagtat
     9961 ttcaaaacca attgtgcttg ctatggcact tatggataat gattgggaag acgttttttc
    10021 aaacgttgga atggagccaa gtggggaccc atttaattca ataatgaaac ttgaaattaa
    10081 tgacacaaaa aaaccctgta atccaatgat aaatgcaggg gctatagtaa ctacttccct
    10141 tataaatggg agttgtttag aagaaaaaga agaaagaatg cttagtttct ttagaaagtt
    10201 agcaaaaaat gataatatag gaataaacta tgatgtctat aagtctgaaa aaatgactgg
    10261 agatagaaac agagctatgg cttacttatt gaaaagtgat ggatttatca gagggaatgt
    10321 agaagatgtt ctagaccttt attttaaaca atgctcaatt gagatagatt ctgtagattt
    10381 agcgagaatt ggaataaatc ttgcaaatta tggagtagat atagaaaatg gtgagcattt
    10441 aatgagtgaa atggtttcta ggattgtcaa gacatttatg atgacttgtg gaatgtatga
    10501 tgcttctggg gaatttgcta ttaaagttgg cattcctgca aaatctggag ttgggggtgg
    10561 tataatggct tctgtgccgg gacgcatggg gattggtgtt tatggacctg cgcttgacaa
    10621 aaaaggtaac agcgtcgcag gagttaaagt tttagaggag ttgtcaaata aactcaaatt
    10681 aaatattttt taaaatatta agaaaggatt tttttgacaa caacatataa tttaatatat
    10741 aattaaattg taaagaaata tgaattaaat aaatttttta ggaggggtca atgaaagagg
    10801 tattggtatt acgagatttg gagtgcataa aggctattgc gcatccacgt agaatagata
    10861 ttctaaaagc atttgacaaa tcgccattgt cagcaaaaca attatctcaa ttattggaag
    10921 aacctcatgc aaaaataaac taccatataa aaatgttata caaagttggt attttagaat
    10981 tagttgagga aaaaataaag tcggggattg tggaaaagta ctattatcca agtgcaaaaa
    11041 atgtggttat agggaatagg atacttaatt tttctttaga caatggggaa gaaaaagaag
    11101 aattgtacat atcaaaattt gaaaatatga gtgaagtttt ttataaggca gcagaggaag
    11161 atgttttaga aaatgaaaat atagttgatt atcatgatat ttctcttact catgatgaat
    11221 tagttgaact tagtgacact atgaagtcaa agatagatga aattttaaat aaaagacaac
    11281 acaatgttga aggttctaag tatgatatag ctatggttat agttcctact cttgaggagg
    11341 aatgtcctag ttagagaatt aattatggat taattaaaga tataatatta gagattgaac
    11401 atcaaaattt acttaataat caaaaaatag tttttggtat gggtaaaaca atgattttta
    11461 atctctattt ttttgttttc ttatacctat tgtatttgta aataatatgt atagtgtaag
    11521 atgtatacac tatctgagaa gtgcttagat aatatacaaa gtatgtaaga ttataatatt
    11581 tgaaattgat taaaatgggt ataattaaag tgtaaatatt aaaattaaaa attaatataa
    11641 attaaatcaa tgctagaaat atgttaaaag aggaatgaga ttagttggga tagaacatcc
    11701 cttagagctt atgaattttt agtgaaaaag tactatcaaa taggaatctc catcttcata
    11761 tgttggctaa atatagttaa ggtggagtag ttcaaaatta aaattgagta caagtcgtag
    11821 tacaaataaa aggagattaa tatgattaaa ttaggaacac atctttcaat agcaaaagga
    11881 tttgctaatg cggctgagac agctgtatat attggtgcaa atacatttca attttttagt
    11941 agaaatccaa gaggaggaaa tgctaaagaa tttgatgaaa aagatataat taaatttcaa
    12001 gaaattagaa aagaaaataa ttttgcacca ctgcttgcac atgcaccata tactatgaat
    12061 ctgggtggta caaaggatga tgtatacgaa tttgcaacaa gggtgataga tgaggatata
    12121 agaaggatgg actcattagg aattgaatac atgtgttttc atcctggaag tcatataggt
    12181 ggtggtgtag attttggtat agatagaata gtaaaaggat tgaacaatgc cataaagggt
    12241 gatgaaaata taactatact tcttgaaact atgtcgggaa agggaactga aataggtttt
    12301 aaatttgagc aattaaaaag tataatagac aaaattgagc ataaagaaaa aataggtgtg
    12361 tgtcttgata catgtcatat tttttcagcc ggttatgata tagtgaatga tttagatggg
    12421 gttttggaag aatttgataa ggttataggc atagaaagat tgaaaactgt ccatctaaat
    12481 gatagtatga tgccatttgg agacaaaaaa gacagacatg ctcctatagg ggaaggaaag
    12541 ataggattaa aagcattaat tgattttatg gaacatccta gtctaaaaca tttaccattt
    12601 ttcttagaaa ctccttttga tgatgaaggt cacaagagag agcttaaaat gataaaagaa
    12661 atactatcta gtaaataaac ctttattaaa aatatgtgaa atactccgcc ctcaacataa
    12721 ttaaggtgga gatacaatca tgaaatgtga taatttaaat taatggatag gaataacaaa
    12781 aattgtatac aaattaactg ggtataaagt atgattatat tatgaatagt atgtaaatat
    12841 attgcaaaga ttctcataat ttgttgtata attaaaggta acatatttat attatataaa
    12901 aaattattaa aagttaggtg gggaaagata tgagaaattt aggcaatact aacatgaaaa
    12961 taaagagagt tggctttggg ggcattccaa tacaaagaat tactcaagat gatactaact
    13021 tagttataaa tgagttagaa aaacaaggaa taaactttat tgacagtgca agagggtata
    13081 caataagtga ggaagcaatc ggaattgcca ttgaaggaaa aagaaataaa tttttcttag
    13141 ctactaaaag tatgtccaga gattatgatt caatgaagag agatgttgaa ataagtttaa
    13201 ataattttaa aactgatttt atagatttat atcaattcca taatgttaaa gaagaggagt
    13261 atgataatct ttttaaagat aaaatggctt atagtgccct tttagaggca aaggagcaag
    13321 gtaagataaa acatattggt ataactagtc acaatttaaa tactatagaa aaagctatag
    13381 aagatggaaa atttgacact atacaattcc cttacaatat agttgaagga caagcagatg
    13441 aagtgtttaa aaaagctcat gaaaaaggaa ttgggataat agtaatgaaa cctttagctg
    13501 gaggtgcttt agacaatgca acactggcta ttaaatatat actatctaaa gattacatag
    13561 atgtagttat accaggtatg gagagtgttg aacaggttag acaaaatgtc gctgtacttg
    13621 aaaatcttgt tctagatgaa aaagataata aggaaattga agaaataaga aattctttag
    13681 gtaaaaaatt ctgtagaaga tgtgaatatt gtatgccttg tgctgtagga ataaatatac
    13741 cattgagttt tttatgtgaa ggttattata ctagatatgg attaaaagag tgggctaaag
    13801 aaaaatatga ggttatggat gttaaaccta ctgagtgtat tgactgtggg ctttgcgaga
    13861 gtagatgtcc atatgaactt cctataagag aaatgcttaa gactgttgtt gaaaagctag
    13921 gatagatttt atttattgaa atcggtgtat taaatttact ccgatttctt tttattagat
    13981 gtggtcttat atacaaatat taaatttaca tagttatagc tattatataa atttaatatt
    14041 tgtttaatac ttattaaata tttgcttaat gctttttaaa aacatttgtt tgagtataga
    14101 cataacacaa ttattattgt tgtattagta tagtttcaaa tactggagta tcaatataat
    14161 gtcgaataac acataaaatc tttatttttc aaaaatatta taggatatat cgtatttaga
    14221 tactggataa agttcaattt caaccctagg gttatcttta tctataaaat caaataaaaa
    14281 tactggctta agttgtttat cattttttat tattccactt ttttcaatac catcacaaat
    14341 actttttgga taattatgta aatcccccat tctcttattt ggaaagaata atttcaagac
    14401 tgttattaga ttttcttcaa cagtttcccc ttgatactgt tggtttataa aacctgcaat
    14461 catgttttcg tatgcaagat acttagaata ttttcccttt gaaggcatcc atgcttgacc
    14521 atttatgtta tgaagcttaa aattagattt actaataggt ctacctggaa ttgttatttt
    14581 aatcaaattt ttcaccatcc ttatacagta tttattataa tctgtatatg atttaaaagc
    14641 aaattgttat ttaaagtgtt tttacagggc ttgtttgtaa aattttccac tttagatgta
    14701 gtaatataag ataagtattt tgcttacaaa ttttaaaatt tgatatatga tggacatatt
    14761 catattagac tattataaga ccttcataat attaataagt ataggtttag taaagagagt
    14821 aaaaagaaat ccatataagc caagtaaaga cttaataaat ggcaatgtaa atgggatatt
    14881 aattataaga aaaaatcaat ctttttgcat taactatagg aagaatgtca aaatataact
    14941 aatgatattt gaagttttag agtttatatt aaaaggtaag aaggttaagt gggtaaaaaa
    15001 ggttgaatag attaaaaagg ctaagtatct aaaagagtta agtaggtaaa aaagttaaat
    15061 aagtaaatga aaaaagctaa gtatctaaat gagttagtag ataaaaaaat taaatagata
    15121 aaaagattaa gtatctaaaa gcgttagtag ataaaaaaat taaatagata aaaagattaa
    15181 gtatctaaaa gcgttagtag ataaaaaaat taaataaata aaaaagacta agtattcaaa
    15241 agagttagta ggtaaaataa ttaaatagat aaaaagacta agtatttaaa ggagttaagc
    15301 agataaaaaa gttaaataag taaatgaaaa agacgaagta tctaaaagag ttagtaaata
    15361 aaaaagttaa ataagtaaat aaaaaagatg aagtatctaa aagagttagt aggtaaaaaa
    15421 ctaaataggt aaaaagacga agtatctaaa agagttagta ggtaaaaaag ttaaataaat
    15481 aaaaaagacg aagtatttaa aagagttagt agctagaaaa ggttaaaaag actaaaaaaa
    15541 ttaaataaat ttaaaaagtc aattagatta aaaaggaata attttatgga taataaggaa
    15601 ttaaaagcat tttgtaattt aataggatta aactgtgtag gagtagcagg ggtagataag
    15661 tatgataatt tagaaaagat acttaaggac agacaacaaa aaggttattt aactggtatg
    15721 gaagaaccaa taattgagaa cagaattaac ccaagacata taatgaagga tgctaactct
    15781 ataatagtct gtgcatttcc atatcatata aatagacaag aaaagaataa aaattcaaat
    15841 ttatcaaaat attgtcatgg aaaggattat cacattgtag taaaggactt tttacaacaa
    15901 atttgtgatt atatttctaa aacagtaaca gattttaaat acaagttatt tgttgataat
    15961 ggaccattag ttgacaggta tttagcttat ttatcaggaa taggatattt tggaataaat
    16021 aataatatca taactgatga atatggttct tacgtattta tagggtatat agtaaataat
    16081 tataaatttg aagaagataa accactagaa aaaagttgtt ttaaatgtgg taagtgtgta
    16141 aaatattgtc cgggaaatgc tatattggga aattatgata tgaaccccaa aaaatgttta
    16201 tcatatataa ctcaaaaaaa aggagatttg tcagagaaag aaaaagttgt tttaaagaat
    16261 ggcaaaaagg tatttggatg cgatatttgt caagaagtat gtccacataa tgttgaaatg
    16321 ccaacaactc atattttgga atttaaagaa aatataatag attatttgga ttatgatgag
    16381 atacaaaata tttcaaataa agaatttaaa aggagatatg gagatagagc ttttagctgg
    16441 agagggagaa atataataaa gagaaatatg gaaataattt tagataagta tgatgactaa
    16501 taataagagt ctgtttcata tactttaatg tgtatttact ttaacacttg ttgtgtaaaa
    16561 gtcatattat atcattaaag ttaaatatgg ggaggtgggg gaatattgaa atataataat
    16621 acatctgaaa taaatccact agattatata ttgggtcaag aaagagctgt agaagctatg
    16681 gaattagggt tgaaaataaa taatccagcg tacaacatat atatagctgg tgaacctgga
    16741 actggtaaaa gtacttatgc cttaaaggtt ttaaatgaat atgcatccaa aaaaaataca
    16801 cataaagatt ggtgttatat atataatttt gaaaacccta gagagcctat tatagttgaa
    16861 ttagaaaagg ggtttggaag agaacttaag aaagatatgg agaaattaat tgaaagtctt
    16921 ttagaagatt ttaaaaatgc gtttgaaagt gaaaattttg aaatagaaaa aaatagactt
    16981 ctagatgaat atgaaataga aaaagatatg cttttgaagc aaattaagaa atatggggaa
    17041 gaaaaaggat ttaaattaaa acagagtaaa atgggaatag tttttatccc tttgaaagaa
    17101 gaggaagatg aaagtgataa gtctgatgaa gagttttaca aagctaaaag agaactagaa
    17161 aatatggcga tacaggtcgt atataaaatt agaaacttag aagaaatagc tgaaaaagca
    17221 gtgttagagc ttgaagaaga gatagctaaa cttgtagtag aacctcatat caagagattg
    17281 tgtgaaaaat atgaaaatta tgataagatt caaacttatc tggaaaatat aaagaaagat
    17341 ataatagaat atatgtattt gttctattta gatgaagagg agttaaaaga taagtatgat
    17401 aaagagcatt ttattaaata caaaattaac ctatttgtag acaatgaaag ctcaaagaat
    17461 aaagaatcag cacctgttgt agtagagata aacccaagtc cagcaaatct atttggaaag
    17521 gcagagtatg attatgcaaa tggaaatata aagactgatt tcacaaaact attatcagga
    17581 gcatttcata gggctaatgg tggttattta gtactttatg cagaccaatt actaaaatat
    17641 acaatgtctt gggaaattct taaaaagact ttgcagacta aaaaagtaat attggaaaca
    17701 caaacagcaa ttaaaccaga aaatatgcca ttggatgtta aattagtact gattggaagc
    17761 cactatatat atgatatttt atatagatat gatgaagatt ttgaaaaata ttttaaagta
    17821 tttgtagact ttgatagtga aatggataaa aatgaagata atgaagaagg aatagcaaga
    17881 tttatagcat accaatgtga taagaataat ttaaggcatt ttactaaatc agcagttgaa
    17941 gaggttatta aatttagcac tagattatct ggtgatttag aaaaactatc aactaagttt
    18001 aataaaataa tggaagttgt aatagaagga agtgcttatg cagagtttag aaattcggag
    18061 tatacagagc agtgtgatgt taaaaaagca ataagtgaaa agagaaaaag aatcaataga
    18121 gtggaaacac atatggatga atcaattgaa aatggattta ctctaataga aacagaggga
    18181 agaaaagtag gagtgataaa tggtctgtca gtcttaagta caggagaata ctcttttggt
    18241 agagcttcaa ggattacagc gaccactagc ccaggaagta aaggcatagt aaatatagaa
    18301 agagaagtaa atatgagtgg ctctatacat aacaaaggag tgcttatatt aggtggctat
    18361 ctttctgaga attttgcaca ggatttacta ttgtcactaa acgcatatgt ttgttttgaa
    18421 caaaattatg gtggaataga tggagatagt gcatcttctg cagaattata tgtactatta
    18481 tcatctctta gtggagtatc aattaaacaa aatattgctg ttacaggttc tataaatcaa
    18541 aagggtgata tacaagtagt tggtggtata agtgaaaaga tagaaggatt ttattctata
    18601 tgtaaaacta aagggttaaa tggtgagcaa ggcgtcataa ttcctagaaa aaatcaaaga
    18661 aatctcgttt tatcagatga agttaatgat gcagtgcgag atggaaaatt taagatatat
    18721 actgttgaaa gagtagaaga ggctatagag atattgacag atgttaaatt tgaagaaata
    18781 aagaaattgg taaaagaaaa attaatatct ttcagcaaga tacaaactgt gtcaaaagaa
    18841 taagtttatt atagaattta atgggtattt atgtataata aaaagcaaga actttaatag
    18901 gtttttgctt tttttgtaaa tatatataaa ttaaattctt attttgtatg ttttatatat
    18961 tgatgtttat tttttgttga tatgactaaa ctatttataa atagtgtttt ataaattggt
    19021 attttaaagg gggataatta tgaaaaatgt aaatctaaaa gagataaatg aaaaagatgt
    19081 aattgtggaa gaagatataa atctaaaagg aatggataag aaagatgtaa ttgagaagga
    19141 agaagtaaat aaaaaaagta tagataagaa agatataatt gagaaagaag agatagataa
    19201 aaaaaatgta aagaaggttg aggttcaaaa agaaatagat aaaatgatta gtaaaaaaga
    19261 tgagataatt gatgagaaag atatagagaa ttttaggtat attgaagaag ataaaaaaag
    19321 tatcaaagta agtaaaaaaa gtactaaaaa aggtgaaacg gagaaaaaaa caaagaaaag
    19381 tagttctaat aaggctacac aaaaacaaag caaagaagtt aaaagtaagt ctaaaattaa
    19441 aagtgaatca gccaataaaa aagatgtaaa taagatttta gatgagttgg aaaaattgta
    19501 cccagatgct aaatgtgagt taaattatgg gacagccttt gaacttttaa tagcaactat
    19561 tttatcagca caatgtactg atgtaagagt gaataaagtt acttcagagt tgtttaaaaa
    19621 gtacaacaca gctagagact ttgctaattt gagcattgaa gaaattagta aagagataaa
    19681 gagttgtggt ctttacaaaa gcaagtctca aaaaataaag gatacatctg aacagctatg
    19741 tgaattatat gatggtgaag tacctgatag cttagaaaag cttataaaat tacctggtgt
    19801 tggaagaaaa acggcagggg ttgtacttag taacgctttt aatcatccag caatagctgt
    19861 agatactcat gtatttagag ttagcaatag aataggaata gtggacgagc caaatcctca
    19921 aaaaacagaa tttgcactta tggaagctat accaaaagaa agatggtcac actcacatca
    19981 tgtattaatt tttcatggaa gaagaatgtg taaagctaga aatccagaat gtgcaagttg
    20041 cccaataaaa gaagattgta attattataa agaacttaat gaaacgaaat aacttttaga
    20101 aagtaagttg tacaagaggg attttctgat ataataatac ttgtgaaaat gttagataaa
    20161 aaactattat atacatgaaa taaaaattat tattaacatt attttatata atagtataat
    20221 attattgtta ttctgtttat aaaataaaat ttgataaata aaagcgatac ttagggagga
    20281 ttaaaatgtc attaaatata gtattagtag aaccagaaat acctcaaaat acaggaaata
    20341 taataagaac ctgtgcagca acaggttcaa ctttacattt agtaaaacca ctgggttttt
    20401 cattggatga taaacaccta aaaagatgtg ggcttgacta ttgggatata gctgatatac
    20461 aatattacga tagctttgaa gaattacaag aaaagtatcc aaatagtaaa tacttttttt
    20521 ctacaacaaa ggctaaacag tcacattcag atgtgaaata tgaagaaaat tgttttatag
    20581 tatttgggaa agaaacaaag ggattaccag aacctttgtt gaaagcaaat atggatactt
    20641 gtattagagt gcctatgttg gatgttgaaa aggctaggtc attaaattta tcaaattcag
    20701 ttgctattgt agtgtacgag gctttaagac aaataggata cccaaatatg aggtagtttc
    20761 aacaagccta aatgagaaag gagcatactt aatgaaaaat ctaaagataa tctgtgacag
    20821 tttatcggac gtgccaaaag atttgttaga taaatatgat atagatatag tttcattaac
    20881 agttatactg gaggaaagag aatacaagga tagaattgat atatctggag atgaatttta
    20941 taaaaggctt agagaagaaa atgtatatcc aaaaacttct caagctactt atgcccaatt
    21001 taaggaaatt tttgagaaat atacaaatga ggatagagag atactttata tagcagggtc
    21061 tgccacagct acagggacgt atcaaagtgc tgtaatggca aagaatgatg tggatggtaa
    21121 aatatataca tatgactcta acttgctttg tatgggaact ggtttacttg taataaaagc
    21181 aggagaactt gcaagtgaag gaaagtctgt tgatgaaata ttacctgttt tggatgaatt
    21241 aaaagagaag gcttttgtta tattttcagt agatacattg gaatatttac aaaagggagg
    21301 tagaatatca tctactaagg cagcaatagg aagtatatta aatataaaac cgattcttga
    21361 aataagagat ggactagttt cgcaaatatc tcaagtaaga ggaaagaaga atgtaatttc
    21421 taagatgatg gaattgataa aatcaaattg tggggatgat ttatcagacc aaattgtgta
    21481 tataggatat agtgatgatt ttaaggaaaa agaacaatta tctaaggtta tgcaagatga
    21541 attaagacct aaagaaatta gatttttcca aattggtgtg tgtataggtg cgcatgctgg
    21601 tcctggtgtc actggtatca tatgcttaaa aaataaataa tatatttttg gatgacatta
    21661 tttatttttt tattaaatta ggatagaata ttaattattg agatgttaaa ttggatttat
    21721 tttggatgaa attaagtgtt gatatattag atttagtttg tagggaagtg tgtaaataga
    21781 taagaatcta ttgcacactt ttttatagta agatttatta aaaaaatttc aaaatgtact
    21841 tgtaaattgt atataaaaaa attataaata ggatgtaaaa gtaaatagga tgtaaaagta
    21901 aataggatgt aaaagtaaat aggatgtaaa agtaaatagg atgtaaaaat atcgtaaatg
    21961 tatgaggagt gaaatttaat gcgtttcaaa agaatattga tattgatgtt aactgtagca
    22021 atgatagctg gaatgttggt tgggtgtgct aataacccaa gcaataatcc tagctctagc
    22081 aaagataata aaagtgatat aagcgataaa agtaattcaa aaaaatctgt atcaatgata
    22141 ctagatattg aaggaacaaa taatgaagct atgaataatg cagctttatt agcattaaac
    22201 aatgctcaaa agaaacttaa tatagataca aacaaagtgg aatcagatga ttcctcaact
    22261 ttttctaata gtatagatat tttatgtaat gacaattacg atttaattat agctgtagga
    22321 gctagatttg caaaaccatt ggagatggta gccaagaaat atccaaagca acaatttgca
    22381 attatagact atgagtatga caagcaacct tctaatataa cttcaatttc ttatgaagat
    22441 aataaatctg gatacctagc tggattaatt gctggaaaaa tgactgaaag cgataaggtt
    22501 ggttttatag gaggaataaa gagttctagt agggataaat ttgaatcagg ttttagagag
    22561 ggtgttaaat tttctaatag tagtataaag gatatttcag ttgagtatgc agatgttttt
    22621 aaagatagca aatcagtaga atctatagct aaaaaaatga tggataatgg agtggatatt
    22681 attttttcta ctactgaaga tgattcaaaa gctgtaatag atgcagtaag ggcaaaaaat
    22741 aaaaaagtta tagcaacgaa taaagaccaa catgagttag ctccagaaaa tgttataagt
    22801 tctattgtaa aagattttga gaatcctact tataatctga tacaaagttt tgtaaaagga
    22861 aattataaag gcggaaaagt tatagaaaac acagttaagt ctggaagtac tgggcttgcc
    22921 caaaatagtt ctcaaaatat tcctccagat gtgttagaat atgttaataa gaacaataaa
    22981 taatctacta taatatccaa gaatacttat gactttagtt atgaggtgaa ttggatgtta
    23041 taatagacat atatagaaag acgtatgcag aggtaggcag acctatctaa atcagggagt
    23101 gaattttgtg aaaataaaaa ggatattgat ggcttgcttt gtagtagttt ttacaatttt
    23161 tatattagct atatgtaact ttgattttgg atttgataaa gtaaataaga ataattctaa
    23221 tatagatagt gctaaatcga aacagagtga gtctaaaagt gtaccaactt cagcagaaaa
    23281 ggataaatta aatagtaaaa gtaagaatga agaagatgac acagaagatg attcatatgt
    23341 aagaaaaact attggagtat tgttagatac cgaaaactta aataacgaat catataataa
    23401 taatgtacta tcagccttaa aaagggctga tgaagatttt gatgttagga taaaacttga
    23461 aaaaccaaag acttctaaaa gttttaaaga gagtatagaa aaactacata agtatgaacc
    23521 agatttgata atagcagttg gagctagatt tgcagagcca attaaagaag ctgctactaa
    23581 atatcccaaa cagcaatttg caatagttga ctatacttat aaagttcagc cagctaatgt
    23641 aatttctgta tcatttcaag ataatgttac tggatattta gcagggctta ttgcaggtaa
    23701 aatgactgat actgggaaag tcggttttat aggcggagtt gagggttcta gtagagataa
    23761 gtatgaatct ggatttagaa aggggcttaa aacttcaaat aaagatgctg agttactagt
    23821 taagtattca aatatatttg aaaaccctga atcagctaga actattgcaa aggaaatgag
    23881 agattctgat agagatgttg tattttcagc tacagaagat gatagtaaaa gagctattca
    23941 ggtagcaaga gaaaatggtg gtaaagttat agctattaat aaagaccaat acgatatggc
    24001 tccagataat gtaataagtt caattataaa agatgtagaa caacctgtct atgaactgat
    24061 aaagaacgtt gcaaaagatg gttttaaggg aagtaaagta atggaattta aaataaaaga
    24121 tggagaactt ggcttgactt caaagagcag tagaaatata ccaccagatg ttttggaata
    24181 cataaaaaaa gagtataaca aagtgaaaga tacatattaa ttattgatgt agaagattat
    24241 aaaaatatac tgtttaagtt tagattagca ataaaaagat atatttatta aatttgtgct
    24301 aactgtacaa aatatattga ttaagtttag attggtgata aaaagatata tttattaaat
    24361 ttagtctaac tgtacaaaat atattgattg agtttagatt agcaataaaa agatatattt
    24421 attaaatttg tgctaactgt acggaatata ctgcttaagt ttagattagt gataaaaaga
    24481 tatatttatt aaatttgaac caactgtaca aagctatact aattaagctt agattaacta
    24541 tataaaatta taacaataag tgtgtgggca gaaaaattct gctacacact ttatttatat
    24601 tcatataaaa aaattatcaa aaaaaagaaa aaggagtaaa aaatggaatt tacaagagaa
    24661 ccatttatac tagatgacga tttgtgtcta gctttggtgg acaaaagaat tagttatcta
    24721 atggaaaatg aattaaaatt aagaaatata gagataataa aaacaataga atgtaaagaa
    24781 ttatatgaaa gcataaaata tcacccggat atatgtattt gtaatttagg aaaaggagat
    24841 ataattgttg cgccgaatgt atataatcaa tacaaagagt tattaagtag atataaattt
    24901 aatataatta agggagaagc taatttatta aatagatacc cttatgatat accttacaat
    24961 attgcgatag ttggtgacta tgctatacac aattttaagt atactgacaa agcaatatta
    25021 gattatattg aaaataataa acttaaaaag ataaatatag agcagggcta ttctaaatgc
    25081 tcaatctgta tagtagatag aacgtctata ataacatcag ataaaggtat ttggaaaagt
    25141 atgaagaata cagaaataga gtgtttattg atagaaaaag gtcacataaa tttatttgag
    25201 atgaattatg gatttatagg tggatgtaca gggttgattt ctaaggataa actagctttt
    25261 tgtggagatg ttaaaaaaca tcctgactat gaacgtataa aaagttttgt agaaagtaaa
    25321 aacaaggaga tagttacact tagctgtgaa aatttactag atataggttc tatagttcct
    25381 ctcatgacaa gaaaggagcg ataactttgg ttgagaagga taatatttct tccatgaaat
    25441 ttttgaggaa taataatgta aacgatagat taatatatac gaatgacatt gtagaagtag
    25501 tgatagattc tgcaaagcaa gaagtgttaa ataaaaaaat acttaatggt actaaagctg
    25561 aacaaagagc tttaaatatt gatgagatat caacggaaat aactagtatg ataataagta
    25621 taatgaaaga gaaacttttg aaagaataca ttctaaaatg ctatgggcgt acatatcctg
    25681 aagataaaaa taatatatac atatattcac tgaacatatt tgctaaaaaa gagattttta
    25741 tgagagaaac agtatttact agggtgagaa attatataga agaaaatgat tttataaatg
    25801 tagaaggttt tattagattt agaatgagag aatttatgaa atatatttct gctataggtg
    25861 atattgcagt tgaggagtat ttaataaaga aagaccaaga tgaatttata agagttttaa
    25921 aatactttat agatactcaa gaagaaaaga ttgatttgct taaagttcat ataatggaag
    25981 ataatacatt tgttttatac gataaaaatg gaaataagat agacagtata gatgatgaag
    26041 aaattataaa tatggtaata agagaaaatt taaattatga ggacttttta ataagtacct
    26101 tattaactct atgtcccaaa aaaatagaaa tattggattt gttaaataat aattgctcaa
    26161 aggaaattat agatactgta gaagcaattt ttgaaaataa agttagtata attatggaaa
    26221 actagaatct aagcatataa aagctagcca tataaaatta aaaaaattct ttttaaatcg
    26281 acacaaaaga cttataattg tatataaagc tatagtataa ctagagttat taagtataaa
    26341 gaatatctac ttgattaatg caatatagct ggcaaatata caattgataa ggagtaattt
    26401 tataatggat aatttaatta ggatactgat attagctgtt attggtggat ttataggtta
    26461 tgttacgaat gtagttgcta taagactgat atttagacca atagagccta ttaaaatacc
    26521 tattttaaat atagaaatag taggtcttat accaaagaga agagcagaaa tagcagcaaa
    26581 tgtaggagaa ataattcaag aagaatttct ttctatggac gaaatacttg caaatatcat
    26641 tactgacgaa gataaggaag aagttgtaag atacataaaa gcaagggtaa aaattataat
    26701 acatgagaaa gtttcattta tcccgagtgg tataaaaaac atgatacaag attatctagg
    26761 agagataatt gagtctgaag ttaagcaaag tatagatgaa ttgagtaaaa atataataaa
    26821 taaagcaaat gaacgtatag atatacaaaa aatggtcgaa gataagatta atgaactaga
    26881 tttatatgaa ttggaagaga taataataag aatagctaag aaagaattaa aacatataga
    26941 gtttttgggg cttgtcttag ggtttttaat aggtatagct caagggttaa ttactatgtt
    27001 tatttaaata tatgagaagt agcatttatt taagtactaa ttatatttac ctatttgagt
    27061 gaattactta ttaatagtat ttagatatta attgtattta cctatttgag
//
LOCUS       DAELSY010000041        26929 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.041, whole genome shotgun sequence.
ACCESSION   DAELSY010000041 DAELSY010000000
VERSION     DAELSY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26929
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.041"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(184..2313)
                     /locus_tag="KPG09_001543"
     CDS             complement(184..2313)
                     /locus_tag="KPG09_001543"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBG0966161.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            2579..2827
                     /locus_tag="KPG09_001544"
     CDS             2579..2827
                     /locus_tag="KPG09_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966162.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSTNKKQKSHSRDIKRKIS"
     gene            2993..4384
                     /locus_tag="KPG09_001545"
     CDS             2993..4384
                     /locus_tag="KPG09_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBG0966163.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKEDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            4499..5968
                     /locus_tag="KPG09_001546"
     CDS             4499..5968
                     /locus_tag="KPG09_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG0966164.1"
                     /translation="MSNVNKKLVIAIGAVALVAIIFIGIMCMQFKGEKIAKNTYVNGV
                     DIGKLTKSQAKQELAKKYKLENVEFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SSFFGNLSKTISTNFGKKSNLVVVINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLTNIDTNLGTY
                     STIFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            6033..6626
                     /locus_tag="KPG09_001547"
     CDS             6033..6626
                     /locus_tag="KPG09_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBG0966165.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            6808..8154
                     /locus_tag="KPG09_001548"
     CDS             6808..8154
                     /locus_tag="KPG09_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0966166.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIRKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            8336..8707
                     /locus_tag="KPG09_001549"
     CDS             8336..8707
                     /locus_tag="KPG09_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBG0966167.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            8700..9389
                     /locus_tag="KPG09_001550"
     CDS             8700..9389
                     /locus_tag="KPG09_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBG0966168.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            9909..10775
                     /locus_tag="KPG09_001551"
     CDS             9909..10775
                     /locus_tag="KPG09_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0966169.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            11017..11826
                     /locus_tag="KPG09_001552"
     CDS             11017..11826
                     /locus_tag="KPG09_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966170.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKKGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGQGSYTFFVDEGT
                     AMEYIGEFKEDKKNGKGNFVFSNGNEYIGHFEEDMMNGIGNMLYNSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            11969..13033
                     /locus_tag="KPG09_001553"
     CDS             11969..13033
                     /locus_tag="KPG09_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG0966171.1"
                     /translation="MKRLIALVLVILIVLFYSPELIEEGYDLKEKFFQSEIWNEFLDK
                     ININNNSKKDKKEQNQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     KLYPDDREELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HINKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            13061..13780
                     /locus_tag="KPG09_001554"
     CDS             13061..13780
                     /locus_tag="KPG09_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0966172.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVKNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            13804..14388
                     /locus_tag="KPG09_001555"
     CDS             13804..14388
                     /locus_tag="KPG09_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBG0966173.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGYVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIIDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLENFITEARERVHM"
     gene            14390..15739
                     /gene="pabB"
                     /locus_tag="KPG09_001556"
     CDS             14390..15739
                     /gene="pabB"
                     /locus_tag="KPG09_001556"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBG0966174.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHLIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDTHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            15729..16472
                     /locus_tag="KPG09_001557"
     CDS             15729..16472
                     /locus_tag="KPG09_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HBG0966175.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            16506..17207
                     /locus_tag="KPG09_001558"
     CDS             16506..17207
                     /locus_tag="KPG09_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966176.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISNIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELASKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            17233..17805
                     /gene="folE"
                     /locus_tag="KPG09_001559"
     CDS             17233..17805
                     /gene="folE"
                     /locus_tag="KPG09_001559"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBG0966177.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            17871..18662
                     /gene="folP"
                     /locus_tag="KPG09_001560"
     CDS             17871..18662
                     /gene="folP"
                     /locus_tag="KPG09_001560"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG0966178.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELEIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            18673..19038
                     /gene="folB"
                     /locus_tag="KPG09_001561"
     CDS             18673..19038
                     /gene="folB"
                     /locus_tag="KPG09_001561"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBG0966179.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            19031..19537
                     /gene="folK"
                     /locus_tag="KPG09_001562"
     CDS             19031..19537
                     /gene="folK"
                     /locus_tag="KPG09_001562"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBG0966180.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDILFFN
                     DVASTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTVKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            19518..20303
                     /gene="aroF"
                     /locus_tag="KPG09_001563"
     CDS             19518..20303
                     /gene="aroF"
                     /locus_tag="KPG09_001563"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG0966181.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLKILKAVKDEVGMAVITELMDVRD
                     IDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            20558..22348
                     /locus_tag="KPG09_001564"
     CDS             20558..22348
                     /locus_tag="KPG09_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBG0966182.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLDCGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            22366..23532
                     /gene="rpoD"
                     /locus_tag="KPG09_001565"
     CDS             22366..23532
                     /gene="rpoD"
                     /locus_tag="KPG09_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBG0966183.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            23697..24386
                     /locus_tag="KPG09_001566"
     CDS             23697..24386
                     /locus_tag="KPG09_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG0966184.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            24400..25497
                     /locus_tag="KPG09_001567"
     CDS             24400..25497
                     /locus_tag="KPG09_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG0966185.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            25559..26131
                     /locus_tag="KPG09_001568"
     CDS             25559..26131
                     /locus_tag="KPG09_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG0966186.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEVLEEMNIEVDIFRVSDEVAKT
                     LPQYYLINYKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(26191..26658)
                     /locus_tag="KPG09_001569"
     CDS             complement(26191..26658)
                     /locus_tag="KPG09_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG0966187.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
BASE COUNT        10965 a         2756 c         4787 g         8421 t
ORIGIN      
        1 taattaaaca acttgtaatt aaacaactta taattaaata aattttgatt aaaaaagcag
       61 attgtattat gaaaaaataa gtatctaatt tataaacaat taataatagt attaattaga
      121 tacaacttat ttaatataat aaaatctgct ttttatataa ctataaaatc ttcctacatt
      181 ctattagaat tgcccataaa taccttcaag ttcttctttt aatacttcag ggtcatttat
      241 accaatagac atggttctag ccagtatatt tgcttggtca taagttgatg tccatgagaa
      301 tacaccacct agacaatttc taagaacata ttgagttttt aagaatattg ataaagcatc
      361 atcatatgac attgcaaaat ctccatcctt aactaaatac ggaacttgag cagtattatc
      421 aaatctatat tcatatccat ttttatttat ataatccctt ctaagctcat cataagttct
      481 tgttatagtg gcacctaatc ttccataaaa tggtataccg agaaggattt tttcagaagg
      541 cattccagca ttctcaagat ttctcaccat tgcatcaaca ctatatcctg gcaaagataa
      601 gtctgaatca aaaagatttg cttgatgttt tctaccattt gggcctgttt cacctgctgt
      661 aaaatcataa ctcataagat taaaataatc tattatagga gctattttat ctatttcagc
      721 acttgaattt atatatcctc tatctcctgt tccagctaca ctaagccatt tatcatctcc
      781 tataacatct cttatggcag ttagtaagag tgtaaagttt tctctatctt gaggtcttga
      841 tgttattcca gatgcactac ttccaggata ttcccagtct atatctattc catctaaagc
      901 atattcattt atcatctgat tgacctgtct tgcaaaatta tatctagatg taggtgttaa
      961 tgctgcatca gagaatcctt cagctcccca acctccaata gctacaatta cttttaagct
     1021 aggtttttca cctttaagag caagtaattg atttaaaaat ctaggataag gaataaacaa
     1081 atttccagac tggtctatct cagcaaaagc aaatataact gcatctaata gttgagcatc
     1141 tacattagtt ggatttccaa gaacatattc tgctacaatt ggatttatat ctggacagtt
     1201 tgcattttca actattggat tgtatgggtc tgttatttgt ggtctaacta atttttttga
     1261 atcttttaaa gttttcatag ctattttttt agtatttttt tcgctagtat aatcgtagtt
     1321 tttataatta tgtttataga aatcttcata actactatct tctttgttat agctagaatt
     1381 tattttgtcg caagattcat ctttatcata gttaaaattc atttcatcac aagacttatc
     1441 tttaaactta tcttgtttgg agttgtgaac tttgttagta tttttagtat gttttttctc
     1501 atggttgcaa tcatctttaa tatcagtctt gtgttcttgt ttacagtcaa gagctttgtt
     1561 caggtagtca tgatgttcat gttcacagtt acattcattt tcaatgtttt tagtatgttc
     1621 ttttttccag ctacaagtat tatcaatttt cttgctcact tcttcatcat tataattatc
     1681 tggtacaaaa cataagtacc agtccataca tgaatatttc ttattacaac tgttaactct
     1741 attttttaat tccttatagt ttttaggata aggtaaaatc actggcattc caggaaacca
     1801 gtttgcagga gtaactatat tatctctatc actagtctga agtgcatcta ctattctaag
     1861 tatttctgga atatttcttc ctgtagttag tggataataa agaatcgttc ttagaatttg
     1921 attattatct ataataaata cagagcgaac agttgatgta tcactcattg gttttgatat
     1981 catgccgtat aacttggcaa ttctcatatc tctatcttct ataataggaa atggaatttc
     2041 tacacctgta agtaaagaaa tattatacat ccaagctaaa tgtgaactgt tactatcaac
     2101 acttagacca attagttctg tatttctttt tttaaattcg tcataatatt tagcaaaaca
     2161 taaaaattct gtagtacaaa ctggtgtaaa atcaccagga tgtgaaaata aaacaatcca
     2221 attaccctta tagtcagaga gtctaatagg accatttgtt gtattggctt taaaatcagg
     2281 agcctttgac cctaaacttg gcaaatttgg catgtaaatc acccctcaat aaattatcat
     2341 ttattttatt ctattaatta atacatatta gtgttactat tttattaaaa gttaagtaat
     2401 ccttatgtca aaaaaagata tttattaaaa agatttaata ttttcctaat ataaatgaca
     2461 atagtaattg ctattttttt actaatttgg tatagtaatg gataaggggt agtgtcaatt
     2521 ttagtgatta tacgaggtga ttggattgaa aaaaattcta agaattttag ttatctccat
     2581 gatgatatgt tttgcaatga cttttgcatt agtgttaata aattcagtta tgggagtaca
     2641 tataggattg gattattact ttttgatggg aagtgtgctt acagtttgtt taacaattgg
     2701 aattgtttta tgcaatgcta gaaaaatatt tgatagcaaa gctatacaca agacaagaac
     2761 gaaaactgtc agtactaata aaaaacaaaa gagtcattct agggatataa aaagaaagat
     2821 atcataattt atgtaaagga aatatttatg tataaaataa tgattgatag gtaaaataaa
     2881 aacacgattt aacttgtgtt tttatttttt ttcaagaatt atgatataat aaccaataag
     2941 aaatttagtt taccataaaa tataaagatt attaaatgga gagggaacta taatgaattt
     3001 tagagattta tttgagaagg ctgtagattt tattgatgaa aaaaggaaat ctatagttgc
     3061 gatttcatta tcaacattag gtgtaattgc tttgataata gtatttttct taagtagtaa
     3121 cgagtttagt gtaggaaatg aagctaatga gttgttaaag attatagaga aaagacagta
     3181 ttctatagct gttgattact acacaagtat agaaaaaaag ttttctgatt caaaaatgga
     3241 aaggtttaat aagtctgttt ctaaaaaaat aaataaattg ctattaaata gtggtgataa
     3301 atatttagat ggagacataa gtcaagaaag ttttattgga ttaataaata ctgtgaaaga
     3361 attaaacaaa ataagtatag atgtggatga cttattagct caagctgata gagttagaga
     3421 aatgtataaa gaagaaaata ctacttatga tattgccatt aactacataa gtactgtttc
     3481 aatattaaat ggtatgggta gtaacctaga tgtatacaaa cagaatatag aaacaatcaa
     3541 agagtctaga gatgtttatg actcagcagt aaaagaccaa aaagcatata agtattatga
     3601 agctattgaa aagtataata aagtattaaa agaagatgaa aagtattaca gtatggctca
     3661 aaatgggaaa gaacaatgta ttgaagaaat gtatgattac tatataagta gagctgaaga
     3721 ggcaaatact tctggagatt atgaaagagc attacaatat attgagtatt taaaagaaga
     3781 ttattcagat gatgaaaagg tacagagttt agaaaaaaaa tataaaaaga atttatcatt
     3841 gtatacctta acatctgatg acattataaa tgtaatagct aaaaaaagtg gcaaagacaa
     3901 ggcaaatcta tctataaatt cacttccaca aatgataaaa aataataaat actattatgc
     3961 tgaagtatat gagtatgata aattaataaa tgaagttttg gtaagtgcaa aaactaaaga
     4021 tttatattcg tataaagatg gtaaaaaaga ttacaaagta gattatggtg atggatactt
     4081 tagaatatta gaagatggaa gttatcaatt tggtattaca aaagataaag ctaagtttgt
     4141 gttaactaat actcttgatg aaaaagaaaa taagtataaa aagattgaga tattagatat
     4201 agagaaagca gatagatatg taaaaagtaa aaagagttta gaagaattgt ttggaaaata
     4261 taaaaatatt tattattatg ctgtagtaaa taaaggctta tttagaggca aagaagttta
     4321 tgctattaat atttacaatg aaaaaatata tgccatatca gaaaatggac taaatgaata
     4381 ttaattaata atattaaatt aatatataat taaaaagttt ttggggtttt aaggcgcatt
     4441 atgtagtaat ataataatag ggatatataa ataattttat tgtaaggaga agtatgggat
     4501 gagtaatgtg aacaaaaagc tagttatagc tattggagct gtagcattag tagcaataat
     4561 ttttataggc ataatgtgca tgcaatttaa aggggagaaa atagcaaaaa acacatatgt
     4621 taatggagta gatattggaa aattaactaa atcgcaagca aaacaggaat tagctaaaaa
     4681 atacaaatta gagaatgttg agtttaatta caatgataaa agttggaaag taaagagcaa
     4741 agatttaaat ttatctactg atctggataa aacagtagaa aacgcatata atttaaatag
     4801 aaagagtagt ttctttggca atctatcaaa aactatttca acaaattttg gcaaaaaaag
     4861 caacttagta gttgtgataa attatgataa aaataaatta aaagcagaaa tggaaaaaat
     4921 agctaaagaa atagatgtag atgtaaaaga tgctacactt gatataagtg gtgaaaaagt
     4981 caaggttata ccagattctg atggtcttaa aatggatatt tctaagagta tggagaattt
     5041 tgataatcaa actaaaaaag gtaactataa aaatgagtta gtagtaaaag ctactccagc
     5101 aaaagtaaaa aaagagcaac ttactaatat agatacaaat ttaggtacgt attcaacaat
     5161 atttaaaact tctcaaataa atagaagtat aaacataaaa ttagcaacag ataatataag
     5221 taatgtatta cttatgccag gagaaacttt ctcatttaat aaacatacag ggaaaagaag
     5281 taaggaaaat ggatataaaa gtgcacctgt aataatggaa ggtgagatgg aagaagatta
     5341 tggtggagga gtttgtcaag tttcatctac gttatacaat tctgtgttat acgcaggttt
     5401 agaaattgtt aatgtaaaaa atcatactat accatcaagt tatgttccaa aaggaagaga
     5461 tgcaactgtt gcagatagtg gaatagattt cttatttaaa aataatctaa agcatccagt
     5521 ttacataaaa aattacgttt caggaaatca aattgtttgc aatatatatg gaagtgcaga
     5581 agataaacaa aatataacga tatcaacaaa attagatggc gtttcacaaa ctacaatgaa
     5641 aagagttaat gaccctacaa tgcctaaagg taaagaaaaa gtagacaaaa gtggaagaaa
     5701 tgcatattct gtatcaacat atagaacatt taatgatgca aatggtaaaa aaattaaaac
     5761 tgaaaaaata gctaattcat attatcctaa gaaggaaggt attatattag ttgggactat
     5821 ggaaccaaaa ccagaggaaa aaccaaatac agacgaaaat aagaataatc aaaatacaaa
     5881 caatcaaaat ccaaataatc aacaaaaacc agaaacacca ccaactgata ataagcctaa
     5941 tgagacacaa ccacaaccgc aagcttaaag ttctcaagaa taggatttat gtagagtaat
     6001 aaaaaatata aatctaaata ggagggatta ctttgaattt aaataatata acgaattacg
     6061 ttcaaagtat ttgcgagaat atttctaatg tattggatgt agatgttact ttagttacaa
     6121 aagatttaac taggatagca ggtacaggaa tattcagagg taaaatagga gagacaatat
     6181 cagagaagtc agtgtactct cttgttttaa aagatggaaa atcctatgtt ataaataagg
     6241 aaatagaaga gaactgtaat aaatgtgcaa atagagaaaa ttgtaaagaa ttagctgata
     6301 tatgtactcc tgttaaaatg ggagaacata tacttggaat cttgggtata gctgcgttta
     6361 atgaagagca aaaagaaaag atactttcaa aagataagga gttaactgct tttgtaagga
     6421 gtatgtctga tttaatatct tttaaactag atgaacttta taaacaagaa gttaagacta
     6481 tagatgacct tgagagggaa gctatagaaa atgccataaa gaaatatgga agtaatacag
     6541 aaggtatgaa aaaagtagct gaggcattaa atataggtat agctacactc tatagaaaag
     6601 taaaaaaatt caacattaaa agttaatagt attgaaaaag aggtaacctt tttatgaaaa
     6661 gaagaggatt acctttttta ttatgagaat ggtggaaaaa tatgtatagt tttgacttga
     6721 catattaaat atgttatctt aaatatatat tttagagtgg aggtgagtat tattttagtt
     6781 aaagatataa aacaagacat agaaagcatg cctagaaaaa ttttagatat atctttgata
     6841 gaagataata aaagtgattt gacttttttt aatatagaag aagaagacgg tgatgaaaca
     6901 ttaaaagcat taaaacagag ttatgtgact acaaattata aagaagaaac tctattaaac
     6961 ttgcaaggag ctttaaattc aaagtcagac acaacgtcta aggagttaaa attagaatca
     7021 tataaaagat ataacaatgc attggattta gcatataaaa attatataac tagtgccatt
     7081 aatatggtta ataaagctct ggagataaat cctaaagata ttgatatact aaatcttaga
     7141 ggtcttttaa agttattaaa atgtgatttt gctaaatcat ttgagagttt ttatactgct
     7201 ttatgttatg agaataatca tatatctaga aaatatgtaa atctaatttc atctgaagat
     7261 tttaaaactt ttttaggaag atacaatcat tctataagat ttataaatga agaattaaat
     7321 tatgagtcaa tacatatttt agagaacata attgaagaag agcctgagct aatagagcca
     7381 tatgtaatct tatctttgtt atatgataaa ctaggtaatg taaaaaagag agaaggatat
     7441 ttagacaaac ttaaagagct agataaagat aatcctgtat ttgaaaaaaa tgaagaagaa
     7501 aaagaagata caagtaaaaa ggaagaaata agaaaagtaa aaagaaagaa aagtatttta
     7561 ccatatatta tagttggctg tgttttaata ggtatgggga tatatttgat tcaaagcaaa
     7621 aagagaatag aaaacttgaa taaccaaata agtagtaaag aggaaaagtt aagtgaaact
     7681 gataaaaagt taggagaaac gagtaaaaaa ttagaaaaaa cgaataagga attaaatgag
     7741 gttaaaaata aagaaccaga aagagagata actgtagctg atgaggaaga tttatattat
     7801 caagctctaa atttaaagaa aaacaaagaa tatgaaaaag ccatagataa ttttaaaagt
     7861 gtagtttctt ctggtaaaac taaaaaatat atttctgagt caatatatca attagcaata
     7921 acaaataaat tattaggaaa taaggatgaa gctataaaat attacaaaaa atatataaat
     7981 acttatacta aaaatgacca gtattatgat gattcatatt acgagttggg tatgctttat
     8041 tatgataatg gcgatttaaa aaatgcacaa cagacttttt atagtttgag aagtgaagtt
     8101 ccagacagta tgtataacaa ttcaaaaata aaagaaatct taagtgaaaa ataggtttat
     8161 atagttaagt tttttgaaac aaccaagtat gtattttatt aaattaaaat aaaaataaat
     8221 ttaaaaagag attgtgtcaa attataactt tctattttaa agaaagatat ttgcttgttg
     8281 ataaaactac aaatatatga tttgaagcaa tctctttaat ttttggaggt ggaaaatgaa
     8341 agttttcaat caattagtaa taattttatc tatctgggta ataggagagt atataagttc
     8401 ctttatacaa ggaattatac ttattcctgg gagtatagtt ggtatgctat tattatttct
     8461 actactacaa tttaaagttt tagacttgag tagtatagaa aatgtaagtg gatttttcct
     8521 cgataatatg gctatatttt ttattccagc aggtgtatca cttataaagt cattagacct
     8581 aataagggaa aatgtattag tgcttttatt agtcatatgt ttaagtacac taatagtaat
     8641 gtatactact ggaattattg tggagaaaat gataaaaaag aaggaagaag gacaaaaaaa
     8701 tgtataatgt attacagaca ccagtatttg gtataatagt aacaatagtt tttttcaatt
     8761 taggaagata cattcaacga aaaacaagta atcctatatg taacccttta ttaattgcaa
     8821 tcgtaggaat aatattgttt ttaagcatat ctaaaatccc ttatgagaat tacaagatag
     8881 gagctgatag tctaaatttc tttttaggtc ctgtcacagt agtacttgca gttccacttt
     8941 ataaacaatt tgaattattt aaaaagcata tgtttgagat tattgtagga ataggttgtg
     9001 gtatagtgat ttcttttgta tctgtattga taattggcaa aatagctaat tcagatgtaa
     9061 gtataataaa ttcactaatt ccaaaatcta taacaacacc aatgggaata tctttagcaa
     9121 attctttaaa tggtatagaa tcaataacag ttgtagccat aatttttaca ggtatttttg
     9181 gtggtatggt tgcttctaca gtattcaaac tggggaatat aaatcatcca gttgctaaag
     9241 gaattgctct tggaacatct gcacatgctt taggaactac aaaagctttt gaacttggag
     9301 aaatagaagg agctatgagt ggtgtttcta ttggagtttc tggtacaatt acagtaatac
     9361 taattccaat tataatgaat tttatataaa aaataaaatt aagacgagta ccttgaatac
     9421 ttaaaacaaa ataaaataaa aaaaatttaa aataaaaact gaacgaaaaa aatgcaaata
     9481 atgaaataaa aaaatcatgt actgaaatta taaaaagaaa aatttcattt aggaattaat
     9541 tataaagaga taattactaa acttgaattt gctaaaataa aataaattac tgcgaaaata
     9601 gatatttaga aaaaaatact ataaatttac tatattgcaa gttaaataat aagcagtatc
     9661 atattatgct agaaaaagtt tcatttaagg cataaatgag atgatttgaa cgtttttaaa
     9721 gattagaata taaaagattg cactagacat atatgaaaaa gataatataa tagatgatgt
     9781 aatagatgac agttcaagaa attataaaaa aaatttattt gaaatattat ctattacatc
     9841 aggaataata tatgtaagag taagctttta aaattctaaa gatttagaaa gattgaaggg
     9901 agaaagaaat gagtgaagtt aagaataccc tacaaaaaca tccagtttta agatattcaa
     9961 caatgctatt tgggatgttg atgacatcta taggaataaa tgggttctta agaccagcac
    10021 atctgcttag tggaggtgct acaggtatag caacatctat aaactatttg acaaatataa
    10081 atgtaggttt attaacattt ttaatcaata ttccaatatt tatcttaggg ttcatatacc
    10141 ttgaaaaaga gttttgtatt tcaagtttgg taaatatgat tgtattttca ttattattag
    10201 gagcaactca ggaaataagt aatattatac ctatacatga tatattgctt caaagtgtat
    10261 atggtggtat attatctggt ttaggagtcg gagttgtatt tagaactagg tcttctcaag
    10321 gtggtacaga tataatagct gcaatactaa aaattaagaa gaatatagaa atgaaagaca
    10381 ctgctttagc aataaatggc ttgatagtac ttacaggaag ttttttattt gggttggatt
    10441 tagcattata cactcttata ggtctatttc taaatgcgta ttcaatgagt tttattaaag
    10501 atgcaatgaa ttaccaaaaa tctgttatgg taatgtcaaa tgaagtcgat ttaatagcag
    10561 aagatataat gaagagttta gttagaggtg taactttttt agatgcagaa ggtgcataca
    10621 ctcatcaaaa aaagaagatt atatatacaa tagtttcatc aaatgagata ccaaagataa
    10681 aagatatagc tttgaagtat gataaaaagg cctttatatc tgtaaatgat gttacagaag
    10741 taaaaggaag agggtttaaa gcaaaagatt tatagaaaat tttacaagct gggagtttat
    10801 aatgtaaatt tgtatattat atctctcagc ttatttaaat ttagtatatg aaaataaagg
    10861 ttttatcaat gatttgtgta tcactttaaa agatatcatt ttaaaattaa aattgaaaaa
    10921 attactcaat atgtagaaaa cataaccact attaggatat aataaattta atagatttag
    10981 taaaatatct aggaataacg ttggaggaat agcattatga atttatctaa agaatatgtt
    11041 aatttgtatg ataattattt gaaatatata aatgaagtaa acaaagagat tagaagtata
    11101 aagaacatga agaaacgtgt tttaaaaaag ggcgattttg ctccagaaaa tgaagtatta
    11161 gaattggaag gaaaaacaat acgcgatata gatgatgtcg atacaaaaaa accaaaaaat
    11221 aaaatatata aattttcaaa tggggatgtg tatgtaggaa aatttttaga aggaaagatg
    11281 gaagggcaag gatcatatac tttttttgtt gatgaaggaa cggctatgga gtacatagga
    11341 gagtttaaag aagacaaaaa aaacggaaaa ggaaactttg tattttcaaa tggaaatgaa
    11401 tatataggcc attttgagga agatatgatg aacggtattg gaaatatgtt atataatagc
    11461 aaagatgaat atattggaac ttggaaaaat ggaaaaaaag atggaaaagg tatttataag
    11521 tggagtgatg gatgtatata tgcaggtgaa tttaaaggtg gaaagatgga agggtatggt
    11581 atttgctata atagtaaagg ggaagtactt tatgaaggag aatggaagaa taatttgatt
    11641 catggtaaag gaacttatat atgggaaaaa ggtaaaaagt atataggtga atttatgcat
    11701 ggtaaaaaac atggacaagg aactttttat ctaaataatg aattggttta tgaagggact
    11761 tggaaatttg ataagccatc catcttcgac agaacattag atgaaatatt ttcattaaga
    11821 ctatagcttt atctattaat agataaagct tttttctaat acttactgta aataagtatg
    11881 aatgatattg attagtatac actatcaata ttaactcaat aaatgtgata ttaaaaattt
    11941 ttgattgatt ttaagaagga gggatatatt gaagagatta attgctttag tgttagtaat
    12001 acttattgtt ttattttata gccctgaatt gatagaagaa ggatatgatt taaaagaaaa
    12061 attttttcaa agtgaaatat ggaatgaatt tttagacaaa ataaatataa ataataattc
    12121 taagaaagat aaaaaagaac aaaatcaaaa tttacaatca aataatactg aaggggagaa
    12181 cttaggagag tcagatataa agaattttga aaaaataagc ttaggagata atttaagcta
    12241 tgttttatca agtattggta aacctggtag aattgatatt agtgaatatg gttttgattg
    12301 gtatgtatat aatcaatatg gaaaagaatt tgcaatggtc ggacttgaaa atgatgaagt
    12361 agtagcactt tatagtaatt ctattaattc atgtgaaaat caagatataa aactaaatca
    12421 agatagacaa acagtgagaa ctaagattac accattaaaa tataagagaa aaggaaatac
    12481 cagatatata ataaactcag aaaatcaata tgatataata agtaaagaag gtaagtatat
    12541 tacaattttc tacgatattc atgaagaaaa tagagtttgc tcatatttaa taattgataa
    12601 aagtactgaa gatgagttta aaaaattata tccagatgat agagaagaac ttaaaaaatg
    12661 ttttgaactt gaagttatag accttgtaaa tagtgttaga aatcaaagag gattaaactc
    12721 attgagatac agtgaacagg caactttgag ttcaagaaaa catagtgaag atatgagaga
    12781 taataatttt tttgaccata taaataagaa gaatgaaact ccatttgaca gaatgaaaag
    12841 agaaggtata gtatatactt cagctggaga aaacatagca gcaggacaga ttaatgcaat
    12901 atatgcgcat gaagcttgga tgaactctga aggacataga aaaaatattt tggggaacta
    12961 taataatatt ggagttggag taatatttgg aggaagctat aaaacatatt acactcaaaa
    13021 tttttataaa taactaaaat atttgaaagg aagttaaaaa atgacatcat ggaaaaaaaa
    13081 gacagtatat aagtgcttaa tagcagtagt cttgttttgt ggaatagtgt taatatctaa
    13141 ttttagtaaa gtttctgctt tgatgatgga tactaatgga aatgttttga ttaaacatgg
    13201 ttcaagggaa aaaaagctaa tagcaattac ctttgatgat ggtcctcatc ctaaagaaac
    13261 tagtcaagtt ttagatgtat taaagaaata caatgtaaaa gctacttttt ttatagctgg
    13321 aaaacatgca aaatggtata aagaaccatt agtaagagca agtaaagaag gtcacgagat
    13381 aggaaatcat actttcaatc atcctgatat aagtaattta agttctagtc agatagaaga
    13441 ggagattgtt aagtgtgaag acattttgaa agaagtaact ggaaaaaaac ctacattatt
    13501 tagaccacca tttggaagtt atagagaaaa agacttaata gaaattgcaa aaaagcatga
    13561 ctataaagtt gttttatgga caggtgtaga tgtgaaagat tggaaaaatc caggagctaa
    13621 tagcattgca gataaaataa ttaataaagt aaaaaatgga gatataatac ttttgcatga
    13681 ttatgcaact aatgatacag ttgaagcttt agatatgttt attccaaaaa tgattgaaaa
    13741 aggttttaaa tttgtaacag tttctgagtt gataaaataa tttttagggg ggagatttac
    13801 agtatgattc ttatgataga caattatgat tcttttgtat acaacctagt ccaatatata
    13861 gaagaattag gggaagcagt tgtagtaaaa agaaataatg aaatcaaaat aagtgacatt
    13921 gaagaactaa atccagaggt aattgtgtta tctccaggac cgtgttctcc aaaagaagct
    13981 ggtatttgca tagatattgt tgagcatttt aaggggaaaa aacctatact aggaatttgt
    14041 ttaggtcacc aaactattgg atatgttttt ggaggagata ttataaaagc acaacagcct
    14101 gtgcatggaa aagtatatag tatcaaccat actaataaag gagtttttag gggacttaaa
    14161 aatcctttaa atgtcactag gtatcattca ctaataattg attccaatac agttccaaaa
    14221 gaattagaaa ttacagcaat tactgataaa ggggaaataa tggggattag gcataagaaa
    14281 tatttaatag aaggagtaca gtttcatcca gaagctattt tgtcagagta tggtcatgaa
    14341 atgcttgaaa attttataac agaagctaga gaaagggtac atatgtaata tgataagaga
    14401 gataaatact aaattaaatt cttttgagat atttactatt tttagaaatg aacatgacag
    14461 ttttatatta gacagtgcta tggataaaga aaaattggga agatattctt ttataagtag
    14521 ccaaccattt aaggtattaa agtataagga tactgatgaa aatcctttag aagttttaaa
    14581 agaagaacta cataagtata gggttgtaaa tgatactaat cttccatttg taggaggtgc
    14641 tgtgggatat ttatcttatg atttaggtaa ttacatagaa aatttaccta gaactgctgt
    14701 agatgatatt gagatgcctg atatgtattt tggattttat aatcatttaa tagttataga
    14761 ccatcttgtg caaaaaactt atatagctac tccaaacata gatatcgaat tagaagaaaa
    14821 gataatagat gatatagaac aaagaatatt aaaagaagag aaaaaaggta tagatagcat
    14881 atgttatgaa gaaaaagaag taacacctat aagacttaaa tctaatttta caaaggaaga
    14941 atttaaaaat gcagttcaaa gtgttagaga gtacataaga caaggagata tatatcaagc
    15001 taatttaaca caaaggttca gtggagaaac agaacttaca agctttgaat tgtataggga
    15061 cttaagaaga tttagcccag caccatttgg ggcattctta aactttgaag atacacatat
    15121 attatcaaat tctccagaaa gatttataag atgtgttaat aagagaatag aaactagacc
    15181 aataaaaggt actcgtccaa gagggaaaga taaagaagaa gatttaagac ttcaacaaga
    15241 acttagaaat agtgaaaaag atagagctga actacttatg atagtagatt tagaaagaaa
    15301 tgatattggc agaatatcta aaactggaag tgttaaagtt ccagagctat ttgtaattga
    15361 accatatgca aatgtaaatc acttagtttc cacagttgta ggtgagttaa aagatgataa
    15421 agatgctact gatgtaataa aagcaacttt tccagggggg tctataacag gagcacctaa
    15481 gattagagca atggaaatta tagacgaatt agagccaacg caaagaaatg tatatactgg
    15541 ttcaataggt tatataggtt ttaatggtga tatggatttt aatatagcaa ttagaacaat
    15601 aatcaaaaat gacaaaaaag tctattttca agttggagga ggaatgactt gggattctga
    15661 cccagatgaa gaatatcaag aaacacttga taaagctaaa tccataatga aggctttgag
    15721 gggatattat gaagaataat gtaggttttg atagtagttt aagtaaattt ggaattggtt
    15781 tgtttgaaac tattagagtt aaaaatggaa tagcagtaga tttagatatc catatagaaa
    15841 gaatgctaag ttcaataaat tgtttggatt tagatataaa ctataaaaaa gatttcttga
    15901 taaatgaaat tgttacatat ataaaaaaag aaaatgtcat taataaagca cttagaatta
    15961 ctgtttttga tgaaggttat aatatatcta ttagggatat tccatataat caagagacgt
    16021 atgagaaggg gtttaaatta gctatatcgc caatagttag ggggaatagt ttaattcata
    16081 gacataagac tactaattat tttgaaaaca tatataccaa aaatatcgca aataagactg
    16141 gttataatga tggtatattt ttaaattcag aaggtgtaat attagaatgt tctatgagca
    16201 atattttctt tataaaagga gaaagagtgt acactcctag tgatagatta ccgatattaa
    16261 atggaataat aaaaaaaagg attatagaga tttgtgacga attacatata gagttgatag
    16321 aaaatgaaat taatatatca gaaattagta gttttgattt tgtttttgtc acaaatagtc
    16381 ttatgggcgc tataaaggtt acagaaattg acaaaataaa atttaataaa gaaaatgttg
    16441 tttttgataa aatagtgaag ttattagaat aaattaaaat attaacaatt tgttggagac
    16501 aaaagatgat aggttgtatt tatgaagtat acaatgaaga gatgactaaa ataatagaaa
    16561 accaaaacac tttggctgta tatttagtag gttctagtaa agacgtagat tttactcttt
    16621 atgatgtaga gataaacgat attgatgtgt tcgtttttgt aaaagaaggg gaaaaacaag
    16681 agagaatact gtttaaaaaa aagggaatag aatttgatgt aaattacttt tcaaaagatg
    16741 gtgtaaaaaa attattatca aatagggaat atttctttgt gaaagaaatg aaagatgcta
    16801 aagtactgtt tgatagggag aatatatcaa atattataaa agatataagt agagatattt
    16861 acttagaagg acccagtaaa atgtcattag aggaaaagtc ttttataaag caagatattg
    16921 gagcaaaaat atcgaattta aaaaataaag aaaaatttga tgtttttgaa tatcattttt
    16981 tgacgaattt atatttaaaa gatataataa ttgggtactt taatataaat gataaatggg
    17041 taccaaaaga caaaaaattg ctaaaagttt taaaaaaaga aaataatgag ctttttgaac
    17101 tagcgtcaaa agtaagtgaa aattatgact atcaaagact tttagatgtt tataattata
    17161 tttttaaaga aattgagaca aaagaggtta taaaactaat cttctagtca caaatcaatg
    17221 gggggagttt aaatgaataa agttgataaa gaaaagatac aacatgcagt aagagaaata
    17281 ctagaggcaa ttggagaaga cccagataga gaaggtctaa tcgagactcc aaatagagtt
    17341 gctaggatgt atgaagagat attctctggt cttagtgaag aaccaagaga ccatttaaaa
    17401 gttctttttg cagatgaaaa acatgaagaa ttagtattgg taaaagatat tccattttat
    17461 tcatgttgtg agcatcatct agttcctttc tttggaaaag ctcatattgc ttatttacca
    17521 aaaggaggaa gacttacagg gctttctaaa cttgctagag taatagatac attggcaaaa
    17581 agaccacaat tacaagaaag aatcactaaa aatgctgcag atataataat ggaagaatta
    17641 caaccttatg gtgtcttagt agtagtagaa gctgaacata tgtgtatgac aatgagaggt
    17701 gtaaaaaaac caggttcaaa aactgttaca tcagcagtga gaggaatatt tgaaaaggac
    17761 attgcgtcta gagccgaggc tatgagtttg attactatga agtagcaatt tattatagat
    17821 ataatcattt attgagatat aaaatttaat ttaagggagt gtcattaatt gtgtttgatt
    17881 atggtaaaag aacttatata atgggaatat taaatgtaac tccagatagt ttttctgatg
    17941 gaggagattt taataacttg gacatagcaa tacaacatgc aaaagatatg gttgaccaag
    18001 gtgcagatat aatagactta ggaggagagt ctacacgccc aggtcatagc tatgtagact
    18061 cagacgaaga gctaagaaga gtaatccctg taataaaaaa acttaaacag gaattggaga
    18121 taccaatatc aatagacact tataaggcag atgttgcaga agaagcatta aaattaggtg
    18181 tcactatggt aaatgatgtt tggggtctta gaaaagataa aaatatggct tctgtaatag
    18241 gaaaatatga tgcagaagtg tgtataatgc ataatcaaga tgggacaaac tatgacaaag
    18301 atataatgga gtcaataaaa gactttttta aggtaagtat agaaatggca atgagttgtg
    18361 gtgtaaaaaa agaaaaaata gtacttgacc caggcgtagg atttggcaag gattttgaac
    18421 aaaatattga agtattgaga agattaaatg aattaaaaga tttagggtat cctattttgc
    18481 ttgggacatc cagaaaatca gtaataggaa aagtacttcc agttgagcct aaaaaaagat
    18541 tagaaggtac aatagctact actgttcttg gtataagaga tggcgtagat atagttagag
    18601 tacatgatgt ttatgaaaat ttaatggcag ctagaatgac tgatgcaata tatagaaagt
    18661 aggtataata taatggataa aatattactt agtaatctag gattctatgg atatcatggt
    18721 gttcttaaag aagaaaattt tctaggtcag aaattctttg tcgacatgga actttatatt
    18781 gattcaagag aagcaggtct tagtgacgat ataaataaat ctgtaagcta tgccgaggtt
    18841 tacaatgttg ttaaagatat tactgaaaat aaacaattta atcttttgga ggctttagcg
    18901 gaaaatattg ctgaagaagt cttgaataaa tttatactta taaatggagt tatggtcaga
    18961 gttagaaagc ctgaggctcc tgtaaatggt atttatgact attttggtgt tgagataagg
    19021 agaacaagag atgaataagg cttatttggg aataggtact aacatgggtg atagatttga
    19081 taatttatca agagcatgtg aacttttgaa aaatagtgat tctatatata aagtaaagga
    19141 atcaagttta tatgaaacaa aaccatgggg atatacagaa caagcagatt ttctaaatat
    19201 gtgtgtagaa atagaaacag aatttgaacc ttatgagttg ttagaatatt gtcaagaaat
    19261 agagagagaa ctacacagag agagaatagt tcattgggga ccaagaacta tagatgtaga
    19321 catactattt tttaatgatg tagcttccac tgatgaaaga ctaacaatac ctcatccgag
    19381 gattcaagat agagctttcg ttttgatacc acttatggat ttaaatgaag aacttataat
    19441 aaatgaaaaa acagtaaaag aacatttaaa cctcctatct gctgaagaaa gagaagaagt
    19501 aaaggagctt gtaggttatg aaagaaaacc tatttaataa taattcaaag atagagataa
    19561 atacaaataa caaaaagata actgtaaaaa aagataaact tataatagct ggaccatgtg
    19621 caatagagag ttatgagcaa cttttggaga ctgctaaatt tgtaaaaagt cagggagcaa
    19681 atattttaag aggaggagcg tacaagccaa gaacatcgcc aaattcattt caaggattaa
    19741 aaaaagaagg tcttaaaata ttaaaagcag ttaaagacga agttggtatg gctgttatta
    19801 cagaacttat ggatgtaaga gatatcgacg aactgtatag tataagtgat ataattcaaa
    19861 ttggttcaag aaatatgcaa aactttactc ttttaagtga agtgggaaaa caaaataaac
    19921 ctgttatgtt aaaaagagga atagcatcca caattactga atggataggt gcttctgaat
    19981 atatagcaat agaaggaaat agtaacatta taatgtgtga gagaggtata agaacgtaca
    20041 atgattatac tagaaataca ttagatttag ctgcggtacc cattattcaa aaggaaacag
    20101 gtcttcctgt tgtggtagac ccaagtcatg ccactggagt tagatatttg gtaaaaccta
    20161 tgtcactggc atcttttgca tgtggagcag atggaataat ggtagaagta catcctgacc
    20221 cagaaaatgc tttgtcagat ggtgctcaat ctctttgttt taatgaattt gaagatttaa
    20281 tgaaatcaat taataattat taaaagaggc aagagcctct tttttattcg taatcattgc
    20341 taaggtaaat ttataagtta taatttgata gttagattat ttaatataaa taaagattta
    20401 atattgatag aattaatata aaaaataaaa attttttgat taataagaag gatatcaata
    20461 aaaaaagtcg aattgtgtat attgagtgat tgacaaattt agaatatagt gtagaataga
    20521 aatgttaaca caaaaaatta caccagaagg tgataatatg aatgatataa aagacattat
    20581 tgaagaaata aaagctagat gcgatatagc aagtataata tctgaatata tgaatataaa
    20641 acaatctgga gctaattaca aagggttatg tccatttcat ggtgaaaaga caccttcttt
    20701 ttatataaat acatcaaaac agatttataa gtgttttggt tgtggtgaag gtggagacgt
    20761 aataaatttt gtcatgaaaa tggaaaattt ggattttatg gatgctgtaa aaattttagc
    20821 aaataaatgc ggtatagaaa ttaacactaa catgaatgaa gaaactagga ttaaaataga
    20881 aaaatctaag aaatttcaag atatccatac ggaagcagct agattttatc tttcaaactt
    20941 attgggaagc aaaaatcttg ggtatgaata tttaagaata agaggactag atgataaaat
    21001 tattaagaaa tttggtttag gattttcttt ggattcatgg aattcactta tgaatactct
    21061 aataagtaaa ggatacaaaa aacaagactt attggattgc ggtctaattg caaaaaatag
    21121 agatggaact aattgctatg ataaatttag aaatagagtt atgtttccta tatttgacta
    21181 tagaggaaac ataataggat ttgggggaag agtattagat gattctttgc caaaatactt
    21241 aaattctcca gacacattaa tttttaataa aaaacagaac ttatacggat taaattttgc
    21301 tagaaaaaat ttagaaagta aaactatagt cttagttgaa ggatatatgg atttaatttc
    21361 gctttatcag tatggtataa aaaatgtagt agcaaccctt ggaacagcct tgactgaaca
    21421 acaaggcatt ttaataaaga gatatgcgga cactgctatt atatcttatg attctgatga
    21481 agcaggaata aaagcgactt taagagctat agatatactt actaaattag gtatcaatgt
    21541 aaaagtgtta gacttaaaag atgctaaaga cccagatgaa tttgtaagaa aatacggact
    21601 tagtgattat aaaaaagcta tggatgtttc tactcactac ataaaatata aaatagatca
    21661 tcttaaaaaa gaatttaaca ttcaaaaaga tgaagaacga gtgaagttcg caaaagaagc
    21721 ttctaaaatt ataaaacaat tgacaagtcc tgttgaaatt gatttttata caaagtattt
    21781 aagcaatcaa atagatatta atgtcgagtc tataaagaga gaagtttatg gcaaaaatta
    21841 caataagcca tataataaca aaaatcaaaa gaaaatagaa gaaaaagtaa ttgaaaaagt
    21901 cgaagtcaga caagacggaa aacaattagt tgaagaaact ctaataaaaa taatgttaga
    21961 agacaaaaaa attagagaaa tagcgttact taaagtagaa gaaagtgatt ttttattgaa
    22021 agaaagtaaa gaaattttaa attacatgat taaaaatcaa gaattggaca aaataactat
    22081 tgacaaattg aaaagtttaa acatatccga agaatactta aaagaattaa attcaatttc
    22141 tttgaatagt ataaacttag agaacacaaa agaaatagaa ggaataataa caaatatcag
    22201 aaaaaactca ttagaagagc aaattaatag cttgttgaga gaacaacaag aattagaaaa
    22261 taataatgat atgaaagagg tagatggtag agttatggaa attgctttaa aaatagttga
    22321 gataaataaa attttaaaga gcttgtagag aaaggaggtt tgagtgtgga aaataaatcg
    22381 aataaaaaag agttaaaaaa ggttactgct aagacattaa tagaaaaagg aaaaaagcaa
    22441 ggttctctga cacttgcaga gataatggaa gctttttcgg agactgaact tgataaggat
    22501 caagtagaaa atctttatga gactttagga aatttgggaa tagaaataac agaaacaaaa
    22561 aattataaag ctgatataga tttttcggtt gctgatgacg atttaagtat aggccactta
    22621 gatgaagatg cagaggcaat ttcacatgat gactcttctg caatagaaat agaaactgtg
    22681 gatttatctt taccaaaagg gataagtata gatgaccctg taagaatgta cttaaaagaa
    22741 ataggaaaaa ttcctctact taaaccacat gaagaagtag aatttgctag aaggatgcac
    22801 gaaggtgatg agatagcaaa acaaaggtta gttgaagcaa acttaagact agttgtaagt
    22861 atagcaaaaa gatatgtagg aagaggtatg cttttcttgg atttaataca agagggaaat
    22921 ttaggtctta taaaagcagt cgaaaaattt gactatacaa aaggatataa gtttagtact
    22981 tatgcaacat ggtggataag acaagctata acacgtgcta ttgcagacca agctagaact
    23041 ataagaatac cagttcatat ggtagagact ataaataagc taataagagt atcaagacaa
    23101 ctacttcaag aacttggaag agatccaaaa ccagaagaaa ttgcaaaaga aatggaaatg
    23161 acagaagata aagtaagaga aataatgaaa atagctcaag accctgtgtc tttagaaaca
    23221 ccaataggag aagaagaaga tagccattta ggtgatttta ttccagatga tgatgctcca
    23281 gccccagcag aggcagcagc atattcttta ttaaaagaac aaatagaaga tgtacttggt
    23341 tcgttaaatg atagagaaca aaaagtatta aagcttagat ttggtcttga ggatggtaga
    23401 gccagaactc ttgaggaagt tggaaaagag tttgatgtaa ctagagagag aataagacag
    23461 atagaagcaa aagcactaag aaaactaaga catccaagta gatcaaaaaa acttagagat
    23521 tatttagact aaaaagatac atcattgatg tttttgtgat aaatgccatg agataagtga
    23581 tatctcatgg ctattttgta gtaaagattt aattttattt ttgtagttat ttcagtctaa
    23641 tatctataac ttggattaaa tcctataaat aaagggaatt gaaaggagaa ttatatttga
    23701 aattaacaga tagattattg aaaatagcat cattagtgtc agatggtaaa aaaatagctg
    23761 atatagggac ggaccacgga tacataccag tatatttgtt aaaagaggga aaagtaccat
    23821 ttgcagtgct agcagatgtt aataaaggac ctcttgacaa tgcacataaa gaagtcatac
    23881 agaacaatct tttagataaa gttgatttac gattgggttc tggtatagag atacttgaaa
    23941 taggggaagt agaagaagtg ataattgctg gtatgggtgg aatcttgata agtgagcttc
    24001 tcgaggctaa aaaagaagta gcacataatg tagaaaaatt aatattacag ccaatgcaag
    24061 cacaagaaga acttagatat taccttttaa acaacggata tgaaatttta gaagaagttt
    24121 tagtgagaga agattttaga atatatgaga taattgttgc caaatataca ggtaaaaata
    24181 ctataataga agatgagata tattatgagg ttggaataaa gcttttagaa aacaaagatt
    24241 ctctttttaa tgattttata gaaaaaaaga taaagacata tagttctata gttaataaat
    24301 tagaagggaa aaatggggaa gctatagata agaagagaaa ggaaagcgaa gttgcaataa
    24361 aaaaacttga aaatttaata aaataaatag gaggtatata tgctattaaa gagtctaact
    24421 agaaaaattg aaaaaaagta tcctctaaac ttagcagagg attgggataa tgtaggtctt
    24481 atagttggag attttgatat ggatgtaaaa aaggtattgg tatctttaga agctaatgaa
    24541 gatgttattg atgaagctat atccaaaaat atagatttaa ttgtgacaca tcatcctttt
    24601 atatttggga aaattaataa aattaatagt ggagatttaa aaggcagact tatccaaaaa
    24661 cttattaaaa atgatatatc actttattct atgcatacga attttgatat agcatttgat
    24721 ggtctaaatg actattttat ggaaattatg gaatttggta attcaaaggt tttagatata
    24781 actaaaagcg aaaatttgta taaattagca gtatacgttc cacataacta ttcagatgaa
    24841 cttagaaagg ttttaagtaa ttcaggagca ggtcatatag ggaattatag tgattgtacg
    24901 ttttcaatag aaggtgaagg acaatttaag ccattagaag gctctaatcc gtttcttgga
    24961 agtgtaaatg atatagaaac tgtaaatgaa gttaaaattg aaactgtagt accacagaag
    25021 cttttaggtg gggtaataag ttctatgtta gatgcacatc cttatgaaga agttgcatat
    25081 gatttgtaca agttagaaaa taaaggtgag atatttggac taggtagaat aagtaaactt
    25141 gataaaagta tgacattaga aagtctttca aggaaaataa aagagaaatt aaatatgaaa
    25201 cacataagag tagttggaaa ccttagtaca gacataacga aagtagccgt tgtaacagga
    25261 gctggttcag aatttgtaaa aaaagctaag agacaaggtg cagaagtact aattacgggt
    25321 gatgtaaagt atcatgaggc acaagatgca cttgatattg gaatgtgtat agtggattgt
    25381 ggtcattttg atacagaaga tatattcaaa aatgttatga agagattttt agatgaattt
    25441 agtgaaattg aagtaataaa gagtaatgta tatttgaatc catttagtac tatttagaag
    25501 tgtttgatta aaaaatacta tataaaaaat aaatttgagt ttaattaggg ggataatgat
    25561 gaaagtagca gtaatttttc atagtgtttg tgggagtaca tatttattag ctagagaata
    25621 taaagaagtg cttgaagaaa tgaatatcga ggtagatata tttagagtaa gtgacgaagt
    25681 tgcaaaaaca cttcctcaat attatttgat aaattacaaa gaatacaaag acgaatttga
    25741 aagtataaat gtaattaaat ctggaaaaga aatattagat tatgatgcta tatttatggg
    25801 ttcaccaaca tattatggaa atgtatctgg accaatgaag atgtttatgg atagtttttc
    25861 tgatatttgg gtaggtgctc ctttgagtgg taaaatattt ggttgttttg ctacagcagg
    25921 ttcacaacat ggaggaggag aattagcttt gcaagctatg aatatatttg ctcaacatat
    25981 gggaatgact cttttgtcag ttccatgttc agtaagagga ggttatccag cgtatggaat
    26041 acttcatata gcaggagata attctgatat aagaccaaat gatgatgcca agataggtat
    26101 aagagattac ctaaaaagac ttaacatata agaatatcat ataaaaatac ctaaaagtag
    26161 ctgtatatac ttttaggtat ttttacttaa ttataaacgt gaagctttac cagaaatatg
    26221 gtcaatttct attttaacaa cagttgttaa gttcatatcc ttctctatgt acttcatgcc
    26281 ttcttcaaag aatcctttag aatatttttt tattatttct acaagagcat ttttcttttc
    26341 ttcattttct acagtgcaag cttttccaaa aactatagca ctagaataag tagtactaaa
    26401 tttatctgga ataacacttt cattagcaac tactaaaaaa gaaactttgt tgtttttcga
    26461 tatattatct aacttatgtc catttcttgc acaatgaaag taaatagaat cattaaagta
    26521 tacataattt acagcaactc cataaggata accattttca gatatagtgg aaaaagtacc
    26581 aaattcacca ttttttaata cttcaacagt atcttctttt gtcatttctc tttttttcaa
    26641 tctcatttct ttaaacataa tatcatcctt tctaattttg atgcttaaat taaattttag
    26701 aacatttata aagattatgc tatgtttttt acaaatttta agaaaaaaat atagtttaag
    26761 ataaaaggta gtgaaagaga agctatttgg aggtttattt atgatattat tgttagagta
    26821 gtacaaacaa tactaataaa tttaatagct ggtttcttgt tatttaaggt agtaagcatg
    26881 tctataagaa ggggaagtta tcgagggtag aagttgttat ttataacga
//
LOCUS       DAELSY010000042        26630 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.042, whole genome shotgun sequence.
ACCESSION   DAELSY010000042 DAELSY010000000
VERSION     DAELSY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26630
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.042"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(145..543)
                     /locus_tag="KPG09_001570"
     CDS             complement(145..543)
                     /locus_tag="KPG09_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0966188.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMMILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(964..3456)
                     /locus_tag="KPG09_001571"
     CDS             complement(964..3456)
                     /locus_tag="KPG09_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0966189.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSINQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            complement(3470..4012)
                     /locus_tag="KPG09_001572"
     CDS             complement(3470..4012)
                     /locus_tag="KPG09_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0966190.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            4255..5769
                     /gene="glpK"
                     /locus_tag="KPG09_001573"
     CDS             4255..5769
                     /gene="glpK"
                     /locus_tag="KPG09_001573"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG0966191.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            complement(5883..6512)
                     /locus_tag="KPG09_001574"
     CDS             complement(5883..6512)
                     /locus_tag="KPG09_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0966192.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            7101..7892
                     /locus_tag="KPG09_001575"
     CDS             7101..7892
                     /locus_tag="KPG09_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBG0966193.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRSVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            7921..9141
                     /locus_tag="KPG09_001576"
     CDS             7921..9141
                     /locus_tag="KPG09_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBG0966194.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            9161..9856
                     /gene="pxpB"
                     /locus_tag="KPG09_001577"
     CDS             9161..9856
                     /gene="pxpB"
                     /locus_tag="KPG09_001577"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBG0966195.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            9886..10872
                     /locus_tag="KPG09_001578"
     CDS             9886..10872
                     /locus_tag="KPG09_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBG0966196.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRSKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLNSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(11091..11657)
                     /locus_tag="KPG09_001579"
     CDS             complement(11091..11657)
                     /locus_tag="KPG09_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0966197.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            12009..13076
                     /locus_tag="KPG09_001580"
     CDS             12009..13076
                     /locus_tag="KPG09_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBG0966198.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESVILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEESKDISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            13095..14255
                     /locus_tag="KPG09_001581"
     CDS             13095..14255
                     /locus_tag="KPG09_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG0966199.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEETELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            14268..16364
                     /locus_tag="KPG09_001582"
     CDS             14268..16364
                     /locus_tag="KPG09_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBG0966200.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGFNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTKEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(16523..16867)
                     /locus_tag="KPG09_001583"
     CDS             complement(16523..16867)
                     /locus_tag="KPG09_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG0966201.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            17159..18049
                     /locus_tag="KPG09_001584"
     CDS             17159..18049
                     /locus_tag="KPG09_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG0966202.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            18267..19553
                     /locus_tag="KPG09_001585"
     CDS             18267..19553
                     /locus_tag="KPG09_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG0966203.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDICEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKKFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(19642..20085)
                     /locus_tag="KPG09_001586"
     CDS             complement(19642..20085)
                     /locus_tag="KPG09_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBG0966204.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(20109..20903)
                     /locus_tag="KPG09_001587"
     CDS             complement(20109..20903)
                     /locus_tag="KPG09_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBG0966205.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(21469..21945)
                     /locus_tag="KPG09_001588"
     CDS             complement(21469..21945)
                     /locus_tag="KPG09_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG0966206.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPNVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(22046..23215)
                     /locus_tag="KPG09_001589"
     CDS             complement(22046..23215)
                     /locus_tag="KPG09_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0966207.1"
                     /translation="MLNIRTLCHEINDWIINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKNYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            23378..24241
                     /locus_tag="KPG09_001590"
     CDS             23378..24241
                     /locus_tag="KPG09_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBG0966208.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDDLYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            24312..24905
                     /locus_tag="KPG09_001591"
     CDS             24312..24905
                     /locus_tag="KPG09_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBG0966209.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(25156..25791)
                     /locus_tag="KPG09_001592"
     CDS             complement(25156..25791)
                     /locus_tag="KPG09_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG0966210.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNIHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(25871..26602)
                     /locus_tag="KPG09_001593"
     CDS             complement(25871..26602)
                     /locus_tag="KPG09_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG0966211.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLYMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQNNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
BASE COUNT         9885 a         3215 c         4303 g         9227 t
ORIGIN      
        1 atggataaga taatctaaat attataaatg tgcttgaaat tagactatac ttgtttttaa
       61 ataatccaat atccatattt tagtaatata ctacaaaaaa agaaggttaa tagatgatgt
      121 aaaatcgtat caaattatgt atgtttaaac cattttatct tcattattat tagaggaatg
      181 cttttttaag tctttatatt cagatatctt aagttcaagt attccttcta tttttatttt
      241 atcacgttcg tttagttgtc tgtatagatt taatatcatc atttcatcat tagtaacatg
      301 taagtaatct tctttatctt cttttacact actattgaca tttaccttct ctttaccata
      361 gagaagccag tcagtcgtaa cattaaaata atcagctatt gacattagta tatcacaatt
      421 aggttttcta tctcctgttt catacttgcc taagttttca aattttaaaa tatccataag
      481 tttgcgctga gtaagttttt tggagtttct caaataagca attctttttc ctaaagtatc
      541 cacaaaatac actcctttct ttttatgagt aatgtctaaa tgacatttga aattaaaaat
      601 atataaattt ataatataaa actactaaat taaagtctaa atgacatttt gcttaaatta
      661 atatgctcat aatatgattt taacatatta tagttgaaaa tatatggttt attttgattt
      721 gtatatataa caatagattt aattgttata aaaatgtaaa ggggtgtatg aatagattgt
      781 ataaatttat ttcgataaac taagattgct ttttgattgt ctgtaaaaga gaaaaagatt
      841 aagataaaaa tagtattata ttgtaattta tattaatcaa ttacaaagat tttatgaatt
      901 tattctttag ggtaaaatat ttaagaataa gataaattta caatataata ctataacact
      961 cttttatcta gttttatttt ctttatagaa caataatatt ataaatgcta gtagatttac
     1021 acagaatact gttatataca tctgtttgaa tcctgagttt agagtagatt gtagtgtatt
     1081 ttctattttt gttctattat tttctatgga atttatataa tccaattttg atttatcaaa
     1141 agcctttggt atttcagttt ttacctcttg tgtctttgct aaaactgctt ggagtgattt
     1201 tttttgctgt tgcattgtat caaggctttt ttccatgtct ttcttttggt ttatagaatt
     1261 atttaacttg ctttgtagct ctttcttttg agattccatt gatcttagtt ttttacttaa
     1321 ttcgttcttt tgagttttat ttttttgtaa ctttgtattt aagtcatctc ttgtcttttt
     1381 tagttgctgt atttgagcat ttaaactttc tttttgttca tttacatttg gtttattctc
     1441 gctggaattg ttattcttat tttctgtaga atcattctta gtgtcgtctg attcagaatt
     1501 actggtatta ctctggtttt gttcatttga gttattttcg ttatcagtag gagtcttttc
     1561 tgtagtaggt attttattat aagcagttgt aagttccttg atggcctcat cttgtttttc
     1621 aagaccttgt tccatacctt ttataccttt gtcaacacca gcaatacctt tttttacacc
     1681 atctattcct tgattaatac cagctctagc ttttaccata ttgtcaatac cagtttgaac
     1741 tcctttaata ccactattta gtttattttc accatcttca ataccgttta ttccactttg
     1801 tgttccatct attccttttt gtacattttt ctgtatttta gtaattatag atggaacttt
     1861 ttcatcaaac attgttgttg atatttcttt tgttatattg gtaatgttgg ttgtatctgc
     1921 tgactgaact ttattaagta aggattttgg aagtttgcca cctgtcatgt ccatatttat
     1981 atttgaagta tcattcatat ttgggataga gacatttttt aaattttttg ccatttcagg
     2041 gtctgatttt aagtcatcta tcatgttttc aagttcttca agttgaacta ttttaggagt
     2101 tgagggcttt ccaactaagt ccataatatt atcttgcaca gataatcctg catgtgctat
     2161 aaatccaatc attatagctg gagaaattga agtaccaatt gagcgtatca aggataatgt
     2221 tgcaagtgct gaattagatt cttctaatct tgtatttgat aacatcatat agtttagagg
     2281 agttcccata gtaaagccca ttcctaatcc cataaatatt aaacttacac atacactaaa
     2341 tacagtattg gtttttagag caattaaaac taggtaaaga gagcctatca tagatacaga
     2401 aaatcctagt aatagtattt tctttgcccc aaacttgtca ataagtcttc cgcttaaagg
     2461 agcagcaata cctgcaaata atcccaatat agctacaaaa tatccaccag aaccagcatt
     2521 tatctttaat gcattttcag catactgtgg aacgaatacc atacccatca ttccaatacc
     2581 aacaattaaa gataaaataa gtactatcaa tattctcgga tttaaaaaat attcaaagct
     2641 taaaataggg tctttagctc tattttcaat aaatatgaaa ataggtataa gtacaataaa
     2701 tgtcaataag tatggataaa ctgatatatc ttgtatagaa tttttaaaat taaaaaagtc
     2761 tatattcata agaccatata atagtgatac gattatacat atcaacatca atgtaccaag
     2821 cttatctatt ttttctttag attcagactt attgttttta atacagaaaa ctccaccaat
     2881 aagtatgatt aaacaaattg gaaggtttac aaaaaatagc cacttccaat tttgagttcc
     2941 aaagatactt agaattgtgc taccaatgct tgaacctaaa atattggcaa taccatatgt
     3001 tgccccaact aaacctaaag ccatgcctct tttattttct gggaaggtag ttccaaattc
     3061 tgctgtagca attggcatga ttccaccacc accaattgct tgaataacac gaccaattaa
     3121 taaaatatag aaattggaaa atagtgatga taatccacat attaatgaac cactaccaaa
     3181 tagaaatata ctaactagat acacatattt tcttcctatt ttatctgcta attttccaga
     3241 gataggaatt acagaagcgt atgttaatgt gtatatggta atcatccaaa ttcccgtttt
     3301 ttcatttact ccaaggctat tttgtataat cgttcttgct ggtgatacta ttccagtatc
     3361 aatagctccc ataaatatac ctaatagata aagtgtaagt actaaaaata tttttggatt
     3421 actgtccttg ttcttaatac tagtattatg attcaaggta aacaccacct taatgtatta
     3481 atttttcaat atagggtttt aaatcttcaa attctatatg aagatatgtt gaatatgaaa
     3541 aaaacatttt tattatgata ttgcatacta atttgtcatt attaaaaaaa ctttcttttt
     3601 ctaaagcttc atatacttta tttgaaagct gttcatgaaa gtcgtctttg tgttttttca
     3661 tttcaaaaaa tctctctgtt tcaaaagtgt ttaaaaagtc attgtattgg atattatgaa
     3721 ctttgtttat aaaatttctt atttcagtaa aaataatgtt tagttttttt atgtaatctt
     3781 cagtgttttt attggagtat tcaattcgcc ttatcaataa atcccattca gattttgtga
     3841 tatattctat tatttcttgt tttgaattaa agtaattata tactgtacct gcacttatac
     3901 cacacctttg agctattttc cttatgctaa tattcgagta atcttcttct aaaatcatat
     3961 cccgagatac ttttaatata tcttctttta cattttctaa aatctttggc atattaccac
     4021 ctcttttatt aaaattatta tgaacactgt ttattatact cctttaaaat ttattaatca
     4081 atatgtaagc tataatgtat aatattatta tatagatatg atagaaaata agtgtctttt
     4141 tataaatatc tattatatta aatttgtact taagtatgat tatttattga aatatgtgat
     4201 attaaatttt tctttttaat aaattaaata ttatatgata aggagaatat ggtgatggaa
     4261 aagaagtatg taatggcatt agaccaagga acaaccagtt caagagctat actatttaat
     4321 aaaaaaggcg aaatagtgaa aattgcacaa aaagagttca atcaaattta tccaaaagct
     4381 ggatgggtag agcatgaccc tatggaaata tgggggtcac aaagtggtgt aatgagagaa
     4441 gttatagaaa cagcaggtat aagaccagaa gaaattgctt caatagggat aactaatcaa
     4501 agagagacta cggttgtttg gagtaggtat actggtaaac ctatttataa cgcaatagta
     4561 tggcagtgta ggagaacatc agaaatatgt gatgaactta aaaataaagg attggaagaa
     4621 tcaataaaag aaaaaacagg tcttttaata gatgcatact tttcagcaac aaaagtaaaa
     4681 tggatattag ataatgtaga aggagctagg gaaaaagcag aacaaggaga gttattattt
     4741 ggaactatag atacatggtt aatatggaat ttaactcgtg ggaaagtaca tgtaacagat
     4801 tactcaaatg catctagaac aatgatgtac aatataaatg atttagaatg ggatgaggat
     4861 atattacgag aattggacat accaataagc atgttgccat tagtaaaacc ttctagctat
     4921 gtttatggtc atacagatga gcgcatgtta tcaggagcta aaatacctat tgcaggatgt
     4981 gctggagacc aacaagcagc attatttggt caaaattgtg ttgaagaagg tactgcaaaa
     5041 aatacttatg gaacgggttg tttcttactt atgaatacag gcagtaacat agttaaatct
     5101 aagcatgggt tacttacaac aattgcttgg ggagttgatg gaaaagtaac atatgcatta
     5161 gaaggaagta tatttattgg aggagcatct gttcaatggc ttagagatga attaaagata
     5221 attgaatctg caaaagatag tgaaatgtat gcaaataggg tagaggatac taatggtgta
     5281 tatgtagtgc cagcatttac aggtcttgga gctccatatt gggatatgta tgctaggggt
     5341 tcaattttag gacttacaag aggagcaaaa aaagaacata tagtaagagc cacattggaa
     5401 tcaattgctt atcagacaaa ggatgtttta gaagcaatgc aaaacgattc aaaattaaaa
     5461 ctaaaatcat taaaagttga tggtggagca agtaataata attttttaat gcaatttcaa
     5521 tcagatatat taaatgtaga tatagataga cccaaaatag tagaaactac agctttagga
     5581 gctgcttatt tagcagggtt gtctgttgga ttctatattg gaagaaatga aattacttca
     5641 aagtggtcag tagaaaaaga gtttaatcca aatatgagtg aagagaagag atgtaaatta
     5701 tataaaggat ggaaaaaagc tgtatcgaga gctttaagct gggaaaaaga agatgaattg
     5761 gatatttaaa aattaatcaa agttaaattt atttgaattt aaaaagagct ttatgtaaaa
     5821 tatgaagctc tttttttgtt gttaattttg catttttata taatcatact aaattatata
     5881 atttattttt cgttttcata taaaaacatt ggtatagaat tgtaaatgtg ttggtcacca
     5941 actactaaga gaatgtctcc ttctaaaaat tcaatatatg gtccaggaga tattataagt
     6001 tcttcacctc ttttaattgc aactattgta gcacctgtat tttgccaaaa tttagtttgt
     6061 gcagctgttt gaccaatata tttgcaatta gaatttatgg tgaactgaac gggcactaaa
     6121 ggattgatta tttcaagtct gttggaatag tctaaaattt tatttatagt atctaatatt
     6181 tcattttcta aattagattt agtatctatt aaattataaa gtgttgattt atatgatgat
     6241 atatttgtaa tatctctata tttttttata aaagaagaag ctttgtctgg agatagtaca
     6301 ataacacctt taccctttat gcttttaaca acgccaatgt cctctaaaat tttgacagct
     6361 cttcttatcg tttctggaga tacattgtat ttaccagcca aaacagagcg tccaaatagt
     6421 actgaatcct cttgtatttc acccgtgtat attttatttg caatatccaa cgctattttt
     6481 tggtatattg gcatcgtata gtttttatcc attgtttttc ccccaataca aaatttatgt
     6541 taaaaaaata tataatctta acaatactat tgtataattt ataggtttat atgtctacaa
     6601 tatgtacaaa tattgtgcat gttttaaaat aatattttaa catgcattta gtatacatga
     6661 ttttattata cttaaaacta tataaagaat taattataaa aatataaaga tattttactt
     6721 acaaagaatg ataattatat agatgaaaga aacaaataaa tgaagaaaaa tgattctgtc
     6781 ttgcatataa agtgattaaa aatgaaaaat actttaaaaa tgaaggttaa ggcccttatt
     6841 ttaagtgaaa ataattaatt attaactaaa aaatggtaaa aagatttttt gaaaaagaaa
     6901 tacaaatgaa tgttcaatta aaataattgc aaaaatattc cttaaatggt aaaattatat
     6961 taattaaatg caatagaata gttgcaaaaa taaaattgaa tacaagtgat aaaatatttc
     7021 atgcaatata taaaataaac aaaatttaaa aattaaaact acttattcta tcaaaatgtt
     7081 agcaaggagg gggtcataaa ttgtataaag tagatttaaa tagtgattta ggagaaagtt
     7141 ttggaacgta taagattggt ttagatgaag aagttttaaa atatatttca tcagctaata
     7201 tagcttgtgg gtttcatgca ggtgacccta gtcatatgga aaaaacagta cagctagcta
     7261 aaaaaaatgg agtgaaaata ggcgcacatc caggtttcct agatttaata ggatttggaa
     7321 gacgtgagat gaaaataaca aagcaggaag ctaaagatta tacaaaatat caattgggtg
     7381 ctttaatggc atttgcaagt tcaaatggat gtaatataca acatgtaaaa ccacatggag
     7441 cattatacaa tatggcagct aaagataaag agttagctat ggggatttgt gaagctatat
     7501 atgaagtcga taaagatatt atactgttgg ggctatataa tagtgaaatg ataaattctg
     7561 ctaaagaaat tggacttagg tttgcaaatg aagtatttgc agatagagct tatgataata
     7621 atggtttttt ggttccaaga agtgtagaag gagcagttat acatgataca aaacatgcaa
     7681 tagatagggt tgttaggatg gtaaaagaag gaactgtaga aactttaaca ggtgaagtta
     7741 tacatatcaa agcagattct atatgtgtac atggggacaa tccaaaagca atagagtttg
     7801 tcaaagaaat aagaaagcgt tttgaattag aaggtataga agtttgtcca ttagaaaaca
     7861 tagaagtttg ttcgtcagag aacatagtat aagactgata acatgagggg gagataaaat
     7921 atgagtacaa aaaatattac agataaaact aaaaataaaa aagatgtcgg agcacttata
     7981 ggggctgcat ttataatggc aacatcagca attggaccag ggtttttaac tcagactgca
     8041 caatttaccc aagattttgg accaaatttt tcatttgtaa ttttaattac aacaatcctt
     8101 tttatagggg ctcaagtaaa tgtatggaga gtaattggtg tatcaggact tagaggtcag
     8161 gatatagcaa ataaaataat accgggatta ggttatttag tagctttttt agtagctctt
     8221 ggaggattag cttttaatat aggaaatgtt ggtggagctg cacttggtat gaatgtaatg
     8281 tttaatatga atatgacatt aggaactgta ttaagtggat taatagctat atttgtattt
     8341 atgtcaaaaa attcaaattc tctagtcgat aagataacta aatttttagc attaggaatg
     8401 ataattatag ttggatatgt agcaatatca aatcatcctc ctgttggaga ggcagtatca
     8461 agaatggtaa aaccagaaaa tccaaaagga ttgatattcc caataataac tctacttgga
     8521 ggaagtgttg gaggttatat aacttttgct ggaggacata gattaataga cggtggaata
     8581 acaggagaag aaaatataaa agaaataact aaatcttctc ttttgggtat attagttgcc
     8641 acaatgatga gagttctttt atttttagca atactggcag tggtttcaaa aggtcttcaa
     8701 cttgacccag aaaatccagc tgcttcagct tttaaattta gtgcaggagc aataggatat
     8761 aaattctttg gacttgtatt atggtctgct gcaattacat cagttatagg agctgcttat
     8821 acatcagttt ctttcttaaa gacactaaat ccttttatag ataaatatga aaaatatttt
     8881 ataatagcat ttatagcaat atcaacactt ataatggcat ttataggaaa accagctact
     8941 ttattaattt tagcaggagc tttaaatgga ttgatacttc caatcacatt aggaattatg
     9001 cttatagcat ctaaaagaaa agatattgtt ggagattaca agcatcctac atggctttta
     9061 atatttggat taatagtagt tttaatatct gcatatacag gaataacttc tcttagcagc
     9121 ctaggtgctt tatttgctta atttagaaga ggtgacattt atggaaacta gatacttgat
     9181 atcaggggat aaggcagtgg ttgctgaatt tggaaatgag atatcagaag atataaacaa
     9241 aaaagtaatt tcatttatga gagcaataga aatatcgaat cttaaaggta ttgtaacaga
     9301 aatggtacca acatataggt cacttatgat aagttataat ccattagaaa tagattttga
     9361 tagtttgatt gagaatttaa aaaaaataga agataatttg gaaagtatag tattaccaaa
     9421 acctaaaata catgagattc cagtatgtta tgatgaggtt tttggtatag atattaaaaa
     9481 cgttgcaagc tacaataatc tcactgtaga tgaagttata aaaatacaca caagcagaga
     9541 gtacttgata tatatgctag ggtttactcc tggatttcca tatctaggtg gtatggatga
     9601 aagaatagct acacctagat tagaagtgcc aaggactaaa atttatggtg ggagtgttgg
     9661 aatagcaggt tcccagactg gtgtatatcc tattgatagt cctggtggat ggcaaataat
     9721 tggtaggaca cctttaaaat tatatgatga aaatagagaa gaacagattt tgcttagagc
     9781 tggagacttt ataaaatttg ttccaataac attagatgaa tttatagaaa tcgaaaaaaa
     9841 taacttaagt atttaaatat cgttatagta cagggagtgg tatatatggg attcaaagtt
     9901 gaattaggag gtttccaaac attaatacaa gacagaggta gagttggcta tggtcaatat
     9961 ggtgtatctg gatgtggagc aatggatgag tatgcacata gagtaggaaa tatattagta
    10021 ggaaattctg aagatgaggc ttctttagaa gttttaatgt taggtcctac aattaccttt
    10081 gatgaatata cgcaaatagc agtaactggg ggagatttag gggctaaaat aaatggaaaa
    10141 gaaattcaaa actggaggtc atatcaaata aatccaggtg atgtattatc atttagaggt
    10201 gtgaaatcag gtgctagagc ctatgtgtca atagctggtg gaatagatgt tccacttgca
    10261 atgggaagta agtcaactta tacaagatct aaaattggcg gatttgaagg tagagcactt
    10321 aaaaaaggag attatataaa tacttttatt caggaaaaag attttacaat aaataaaaaa
    10381 ttaaattcaa aatatattcc aacttatagt tcagaaatcg tactaagaat tgtaaaaggt
    10441 cctcaatttg atgctttttc taacggtgaa gtagagaaat ttttatcaaa taaatacaaa
    10501 gttacgaatg aaatagatag aatgggttgt agactagatg gagagagtat aaagcacctt
    10561 aatggagcag atattatatc tgatggtatt tcttatggag ctatacaagt acctggtcat
    10621 ggaaaaccga ttataatgtt atcagataga cagactagtg gtggctatac taagataggg
    10681 aacgttataa gtgtagattt gtataagtta gcacaagcaa agccaaatga tgtagtaaaa
    10741 tttgaacttg tagatatata tgaagcacat agactattga gagagcaaga agataagatt
    10801 caagatatct acaagagtat gaaaaatata agagttgtaa aggcaaaagt attaaatgat
    10861 atagcagttt aatttaattt taacaaatac aatttttatt ataatttata atataactta
    10921 caaatagcta tttactattt accataatta agatggttta gattagtcaa atagctattt
    10981 ttttagtttg agtaaatatt ttaaatttaa ataaatactt caattttaaa ataaaagcaa
    11041 tatttaaatt tattatatta tctacaaaaa gtaggtgtgt tatgttgagt ttatttagat
    11101 tttattggcc aaattggagg tgatataact ccatatccac gagctattgt atctttactt
    11161 ggattgatga ttttatgtgg tacattagct tttataaatg ttgagtcacc tggatttaat
    11221 ctaactacct tttcacccat acaaatatca accacaccac tcgttaccac cataatttct
    11281 tctgatgcat gatagatata atctttagca tcctcacttt ctggtttaag ttcaaactct
    11341 acaaataaaa ttgaaggtgt aaattcagtt gtaggcattg gagatactat ctcgtaaaac
    11401 atttcactct ttggaaattt catcataact ctatcttctt ttctaattac taaatcatca
    11461 gtattattgt catccataaa catatataaa ggagtgtcta aaacttcagc gatttttctc
    11521 aatgaagaca aggatggttc aatagcatct ctttctattt gagagatata acttatagat
    11581 aaatctgtca atttggaaac atcacttaat gtttttttct tgctttttct tatatttctc
    11641 atcttttcac ctagcataag aacctccaaa taataaaatt aatataaaat attcgcttaa
    11701 aacaaagtaa ttatagcaca acatagaagt agtggcaata agaatacata gtagattttg
    11761 acaattaaaa tttaattaaa tccaaataaa agtctatgaa aatgaattat tgttaaatta
    11821 atataaagaa aataataata aaaaaattta atagaagagt attaattgaa ttaagattat
    11881 cacattatat tttggaaaag agtaaatttt cagtaaatta tcttgacata tatattgaag
    11941 taataatata atttaaagta taaaatttta aaaattgaat tataacttca aaaaataagg
    12001 agggtaatat gaagataaca gatatcaaat ttgaaaagct tagaattaaa ttaaaaaaac
    12061 ctgtagtagt ttcatttgga gtaattgaat atggagaaag tgtaatttta aaaattgaaa
    12121 ctgatgaagg gtattgtgga tttggcgaag cagcacctct tgcagcagtt acaggtgaag
    12181 ttttggataa tgttctttca gtattgctta tgtttaaaaa agagttaata ggcaaagacc
    12241 cactagatat tgaaaccata catacaatca tggatggaat tattataggt aatacttctg
    12301 caaaagcagc tattgacata gctttatatg atattaaagg aaaaattatg aatgcacctc
    12361 tttataaagt tcttggagga tttgatagta aagtccaaac ggatattact ataagtatag
    12421 ataagccaga aaaaatggca gtagaagctt tagaaagagt aaaagaaggt tttaggatat
    12481 tgaagcttaa agcaggtata aatcctgaag acgatattga agctgtaaaa ttaattagag
    12541 aagccgttgg agagagtata agaattagaa ttgatgcaaa tcaaggatgg aacgtaaata
    12601 gcagtataaa tactatcaaa aaattagaag aatttaatgt tgatgcaatt gaacaagcac
    12661 ttccacattg ggatttagat ggtactgctt acataagaaa taaaagtaat acaaaaatta
    12721 tgatagatga atcgttacat tcaccaatag atgcaattaa ggctataaaa aagaatgcag
    12781 ttgatacatt taatattaag ctgatgaaat caagtggaat atatcctgct ataaaaatca
    12841 acaacatagc tgaggcaagt ggagtaaatt gtatgttagg atgtatgctg gaaacgagaa
    12901 ttggtataac agcagcagca aatttaatag catctaaaaa gaacataaca gaagctgacc
    12961 ttgatagttt tatgttttgt gaagaatcaa aagatatatc tggaggattt gtaatggatg
    13021 gagatattat gaatcttgtc aataaaccag gtttaggaat agaagtaaac ttatgataaa
    13081 tttggaggtt aaatatgaat acactttttt caaatccagg tagtatactt gctgtaatga
    13141 catcaatgat tgcattgggt ttttacttac aaagatacaa agccattaaa agcttaggac
    13201 cagcacttac aataatcata atgggaataa tactttcaaa cttaaaagta gttccagtaa
    13261 gtactgagtt atatggaact atttcaacat atgctatccc agtttctatg actataatgt
    13321 tgatgagtgt tgacttaaaa gaaatgacaa aattatcaag agaaccttta attgcaattt
    13381 ttgtagctgt attaactgta agtatcatgg catttttatt tggattagta tttgcagaaa
    13441 aaatatcaga aggttggaag gttgctggaa tgtttgtagg tacatacact ggtggaagtg
    13501 ctaatcttac agctattgga acaggcctta atgtaagtag acaaactctt gctgcagcaa
    13561 atgcagcaga ctatgtaatt ggagtaccaa cattgatatt tatgtttgct ctaccagcaa
    13621 tattaaaaaa ttcaaagaag tttaaaaaac tttggccata tcatgtagaa gaaacagaat
    13681 tagaagattg tcaaaatgaa gaatttatgg agtcaaaaga atggagtatt aaagatatag
    13741 cttggatgtt ggctataggt tttgttgtta cagaagtttc aacaatactt gctggatatt
    13801 tcaattcaag ttttagtagt gcagcaagaa tacttttagt tacaacaatt tcaattataa
    13861 ttgctcaatt aaagccagta aagaaactaa agggaaattt agatttagga ttatttgttg
    13921 cattattctt tttatgtaca ataggttttt cagtagatat aaaagagttt ttaggttcaa
    13981 cttttactat aacactttac tgttttagca taatttttgc ttcatttgtt tttcatttag
    14041 gcataactag acttttaaag ataaagtatc aatatgtaat actttctata gtaggagcaa
    14101 ttgctgatgg tcctacttca gctttagtag ctgcttctgc aaagtggaat tcgcttgtaa
    14161 gtgtagcagt cgtaatggga gtaattggtg gagtattagg aaattatgca ggtatatcag
    14221 tggcatatgc aataaagatg tctctaggat tatagggggg agtttaattg ataaaattta
    14281 aattatacat atctggacta tacagtggaa atgtaatctt tgatggcgat ttattaattg
    14341 aaaagttgaa tccttttaca aataaaatag aaagtttaaa accaataagc aaagaagaaa
    14401 atacatacta tttaaatctt acaaaaatag atttaaagtc tttatttaat aactttgatg
    14461 tatatactaa aagcttagta aacacagata aagacacagt tataaataac ttaggagaaa
    14521 cgcattctaa acttaatgaa tatatatggg ttcaaagaaa caaaaagttc ccactagata
    14581 taattattgt agataataaa atagtaggtt ttatctgttt atccagagaa acttgtacta
    14641 tattaattat ggatggatat gaagagtata ctgtattaaa agaatgggaa aaaacccata
    14701 aaaatgaaga gatatattct ataagatttg gtggaaatta tatgattgat atgaaagatg
    14761 gtataaaact ttctacagat gtatatctcc ctgattttgt agattctacc aaaaaagcac
    14821 caacaattct tatgagaact ccatatggaa aagaaaatga taaagaaata tattataaat
    14881 atgtacaaag aggatatgct gttgtaatac aagatgttag gggaagaaat gaatcagaag
    14941 gtaagtggga acctatgatt catgagaggg aagatggaga tagtaccata aattggatag
    15001 tatcacagga atggtcaagt ggaatagttg gaatgcttgg agcatcatat cttggttatg
    15061 tccaatgggc agctgcatct tcaggcaata agcatcttaa agctttagtt agtatagtaa
    15121 catcaggaag cccttttatt gatataccaa gaaaaggagg agcttttgta tctggtatgc
    15181 ttgcatgggc atttatggta tcaagaaata aagttgatag aagtaaaatg gtaagagatg
    15241 attgggatga tgttttaaac atacgaccaa tagaaaatat acctgttgaa gctcttggat
    15301 atagaattga atttctggaa gaatggttaa agagagtaga aaaagatgag tattgggatt
    15361 tgatggattg gcatttacaa aaagataaaa taaatgtacc tgcattggtt gtatctgggt
    15421 ggtatgatga taatagtatg ggaactacag aggccttaga tgttataaag gactatgaaa
    15481 aaggaaagag aaaggctatt ctaggtccat ggatgcataa ttcaaatact ttaagagata
    15541 taaatggtat ttctttagga aatagttcat taagatatga tttggattat aattatctat
    15601 tatggtttga taaatactta aaaggtatag aaaataacat agatacgact gctccagtag
    15661 agtattactc tgtaggtttt aataagtgga agacagaaga aaattggcca ataataaata
    15721 agatagataa aagtatgtat ctaataagtg atggaaatgc taacacatct ttaggaaatg
    15781 gtagattagt atttgataat gatttagaag aaaagtatga tagttatata tataatccaa
    15841 aagacccatc agtacaacta attgatatgt cagagaatga agttggagtt ccaaataact
    15901 acaaagattt agaaaaaaga agtgatatgt tgtgttatac atcagatgct tttagtgaag
    15961 aatttactgt aacaggtgat ataaagttag agttttttgc atcaagttct gccaaagata
    16021 ctgactgggt tataaaaatt atggacgtgg atttagatgg aaattcaatt aaattagctg
    16081 atggtatact tagtgctaga tttagaaata gtttttataa gtcagagttt atggaagaag
    16141 gagagattta taagtttacg attataactt caaaaatatc aaacacgttt aaagttggtc
    16201 ataaaattag actagatata acgtctagtg ctaaaaactt tattttccaa aatagcaaca
    16261 cgaaagaggg atataatagt attgaatata tagaagcaaa gaatacgatt taccatggag
    16321 gtaaatatcc atctaaattg atattgccaa tagaaaataa gtaaatttat atagattaaa
    16381 tagtgaggag gtgtctcaaa atgaattttt tagttcgtga gacactttct tttatacgaa
    16441 atcaaatata ttttcatcat ttcaacaatg aaaaagaggc ttatctctct gttttgagat
    16501 agcctcctat aagctaaatt gattattttg tagctgcaac aacgctcatt tcaactaaca
    16561 aatgctctct agcaagtcta gcttcaacac aagctctagc aggttcaaat cctttttcaa
    16621 cccaagcatc ccaaacagag ttcatagctt caaagtcttt catatctctt aaataaatag
    16681 taacagatag caaatgttgc ttatctgaac catatttgtt taataaatct tctattttag
    16741 ctaaaacttc agttgtttgt tcaattactc ctccttcagc atgagtttgt ccacataaat
    16801 aaactgtatt gttatgaact acagctctac tcattcttcc agttccttca tatctcttta
    16861 tttccatatt ttaaatacct ccatagttaa gaaatctatt aaaaatctaa ataataaatt
    16921 aaaaaaagtc tatactatat aaaattatat tatatattct gataataatc cataaaaaca
    16981 tataactttt tagctataat tattattgtt attaattgaa aagacttatt agttacttga
    17041 catagtttac acttttaatt atagtataaa ttggaatatt tagataatta ttatttaatg
    17101 tataagctaa gtttttaatt aatatggctt tatacattta tatagaaaag aggtacgtat
    17161 gagtagcaca atcaaatcaa atcgaataaa aggtataatg tttataatag catcagcgct
    17221 tggctttgca atgatgtctg cattcgtaaa gctagctgga gatttaccaa gttttcaaaa
    17281 agtgttcttt agaaatctgg tatcagctat aatagctttt tggcttatca taaaacataa
    17341 aggaagtcta actggtaaaa aagaaaattt aaaagtactt ttatataggt caatatttgg
    17401 aactctagga gttatattta actactatgc aattgacaag ttgatgcttt cagatgctaa
    17461 tatgctaaat aaaatgagtc ccttccttgt agtaatattt tgtgcaattg ctctaaaaga
    17521 aaaaataaat gtaaagcaga taggagctat tataattgca tttataggag ctttattcat
    17581 aataaaacca acatttagtg tagaagtaat tccatatctt gggggaattg caggagctgt
    17641 atttgcagca atggcatata cttgtttaag agttcttgga gatagagaag attactatac
    17701 aattgtattt ttcttttcag ttttttcatt agttactgta gggccaattg cttttgcagt
    17761 atatgaacca atgaccctta tgcaacttat atatctatta ctaggtggag tatttgcaag
    17821 tctaggtcaa tttggaataa ctttagcata taaatatgca cctgctaagg aaatctctat
    17881 atttgactat agtaatataa tcttttcagc aattttaagt atattcttat tcaatgtcta
    17941 tccagatatg ttaagtgttg ttggatattt aatagtattt tcggcagcat tttatatgtt
    18001 tttatacaat aagaagttag ataaattaga caaagaaaag aagaagtaaa ctagataaaa
    18061 gtgtttttat aatttgtatt gaatatttaa gtctttgtat taaaattaaa gaaaatttaa
    18121 ttaaaaatgt ttggtatatt aacttataac agcagttaaa attgaaaaat tataactgct
    18181 gtttctttta ttaaaaatat ccaaatgtct gtaaaaatag actataatag agtagtataa
    18241 ataatataaa ggggataaat taaagtatgg aaaaagtgaa agttgctgtg attggagcag
    18301 gtaatagagg aacgtatgca tatgctccat atatttatga aaattctgat atatgtgaaa
    18361 tagttgctgt agcagaacct aaaaaaggaa gacgagaact atttacacaa aaatataacc
    18421 tagatagtaa aaatgtattt gaaactttag aagacttttt taaacatgac aagatggcag
    18481 atgcagtaat aatagctaca aatgatgata gacattatga tgtagcaaaa ctagctcttg
    18541 aaaaaggtta tcatgtacta ttagaaaagc caatgtctaa tagtttagat ggtttggtac
    18601 atatagatga cttgtgtgat aagtataaag ataagatttt tatgatttgc catgtactga
    18661 gatattctcc attttataat aaattaaaag aaattgtaga aagcaaaaaa ttaggagagc
    18721 tagttagtat acaatataat gaaaatatag gatattggca ttttgcacat agctttacta
    18781 gagggaattg gagaaatagc aatgaaacca gccctttaat tttagcaaag agttgtcatg
    18841 acatggatat tttgttatat ttagtaggaa gtagatgcaa aaaaatatct gcatttggaa
    18901 gcttaaagca tcttaataac caaaatgcta gtggagagat ggcacaaaat tgtcttcagt
    18961 gtttagttga aaaaaaatgt ccatattcag caaagagaat ttatttagaa aaagatagaa
    19021 gtataaatag agctgttcac ataaatccaa ctgaagagaa tctattaaat atacttaaga
    19081 caagtccata tggaagatgt gtttacagat gtgataataa tgttgtagac aatatggtaa
    19141 atatattaca gtttgaaaat ggagtgacgg ctacttttaa tttatgtgca tttactaaag
    19201 aaaatggaag aactataaag ttaatgttta gtcatggtga agttggtggg gatttaaata
    19261 aaaatgagat aagaattaaa aaatttggaa aaaacgaaga aatagttatg aatccatcaa
    19321 atcaacagaa tatggtagag gaatatgata gaaatcttat agctgaattt attaaattag
    19381 tttcaaataa agaactagaa aagggtagag tagcagcaaa agaagctata caaagtcatg
    19441 taatggcatt tgcagcagaa tattctaggg tcagtgatga agtagtgtat atagaagaat
    19501 tttttgatag tgctaagcaa atgactaagg agatagaaga aactatattt taattaataa
    19561 aaatttatta aataaaataa agatagatta aataatagaa atacgaaaat atgtgttcta
    19621 ttaaggaatc tatctttttt attaaggttt tttcttatcc ctatcaaaaa aatccataca
    19681 ttctgataaa agataagaaa gtaaagcgaa taagatttct gtacttaaag gaatcgttac
    19741 tgaatccata aaggtatcaa ttatgttcat agctagaaaa gttagtataa aatcaattat
    19801 aaattcgcaa agtttatata ttgagtatgg taatttgttt acatatctga atatatctaa
    19861 aatagtagtg catagaaaat caattggagt ggttatacaa aaatatatta aaaagaaaat
    19921 taatacagct ccaagagatt tatattcaaa tcctaagaat gataagaagc caattcctat
    19981 aaccgtgaaa atacaaaaag ttataccaaa aaaaacagca aaaaataaaa atacagaaat
    20041 aaattcgcct aaaggaatcc ttcttttatt acttatctta atcaactcct taatttatga
    20101 tatgaaattt atataaataa taacattctt aaaaattcat aatagtcctc tgtttcaaat
    20161 agtactgttt gagaatctat ttttttcata tttggataaa aagaggcctt aaaagcttta
    20221 taatgttctc taaattttat ttctatctta aatttatctg gcaattttat taagtgccta
    20281 gataaatctc cagataaagc ttttttgaca cctttttcaa ttttttttag tgcaaggttt
    20341 ggatggattg aaattgaacc atttccaatt ccttttgaca caggaacagt gactatattt
    20401 ggatttagtt tttttgcact ctcacaaagc atttcatctc cacttaaaaa tgctacagga
    20461 acattataat atgcagcaat ataagcattt atagtaaatt cagatgctat ttcatcattt
    20521 attttaaaat agtcataatc aatatccata gtatgagaaa gtggattacc attacaagta
    20581 gctccagagt gatagccggt gaatatacat gcatcaaagc tttcatcaat tccagccatc
    20641 ataactagtg ggtctttggt ccatcctcta agtattttga cgttttcagg taaaatattt
    20701 ggattaatat ttctaccact gtcatgtgca tctttaacaa gtatttcatg tacaccagat
    20761 tgagtagctc catcacatgc tgctgttatc tctttactca tttgtgttct atagtgttgt
    20821 gaaaattcac attcaagttt tgtttcatcc caatttacaa taccacaagt accttctata
    20881 tcagcactta aatacaattt cataatatat acccccttat gtttattatt gatgataata
    20941 acagataaca gaaaatacat aatcaataaa gggcataaaa atattatgtc ttactattaa
    21001 ttattattaa atctgtaact gaaaaataac taaattggta ttaaaaaata taacactatg
    21061 tgtcatttat ttatatagtt ttttatatct gatgtttata taaaattgta acatataagt
    21121 ggttacattt actagtttaa catagttaaa atagttaggt attattttta agttaaattg
    21181 attattataa aatcgtagaa taaaagaaaa atttaattta ttaaaatgca tgaaatgttt
    21241 tttcctcaac ataatgctaa attaaactca aaaagaaata atagaaaaat cttatgtagc
    21301 ttagaaagaa tgtgaatgat ttaagggaaa aatagaaatt tattatataa tattaggctg
    21361 tattatgtaa tacagcctaa tattatatgt atatgtgaca taaaatcttg gtaagtgtaa
    21421 ataaaataaa tgtggaaaac aaattaatga aatcaagaaa aaagtaattt aaaattcaat
    21481 ttcatcttcc caattaaatt ctcctgattt ttctacactc ttaggccagt gactacacca
    21541 tttaggaacg gacggatttt caaccctatc aacgcttgga gtatatggct ttaagtctat
    21601 aacaggacta ttatcattag catcaatata tggtattttt attataccat tttcatggtc
    21661 gatattgata atttgtacag cagtcaatgc tattggattg ggtcttatag gtgaccttgt
    21721 tgcaaatatt cctataacgt ttggtccact tttataaggt tttggggttt ctaaaatact
    21781 tcttagttca ggattatcaa acccatcagc ccaccaaaat acatttaaat gactgaaatc
    21841 gtcgagttca tttaatgcag gtacatactt tttgtctaat ttaataaaga cctcttcgcc
    21901 ttgaatacat attttaccta taggatttat taatatttct ttcataatat tacctccaac
    21961 tatttaattt atcatttgta attttagggt ataacatgac atcatgtgag ggtcaatata
    22021 aaataataaa taaaaatatt aatagttaga atgataatac attttttaca tggagcatga
    22081 caccagtaat aaggcaatct tcgtcaatat caaaagtaga ggtatgcaaa ggggaagtaa
    22141 tgtttttttc acgatttgca caaccaaggt ggtaaaaagc tccttcacag tgtcttagaa
    22201 aatacgaaaa gtcttcagcc ccaagagtgg gcgagtcttt aaggataata ttttcttctc
    22261 caaacaattc tacagcattt tgcttgacgt aatctactag atggtttgaa ttgataagag
    22321 ctggataacc ttcctcaatt tcaacagaaa tacttcctcc aaatgtttta caagtaaaat
    22381 cgcaaatttc tctaattcgt ttttttgaaa actctctagt ttcaggagtc aaggttctca
    22441 aagtacccct tataaccact ttgtcacaaa ctatattttc cttgattcca ccttcaatct
    22501 ttcctagaga aataaccaca gaatcagttg gatttgtgtt tcgactaacg attgtctgta
    22561 gagctgtaat aatatgagca gatataacaa tagcatcaat accttgatgt ggataagctc
    22621 catgacattt tgaaccatgt acagttaatt gaagtgtatc ggtggaagca tttagagtgt
    22681 tgtattttaa ttcaattaga tttttgttga tattgggatt tacatggagt ccaaatatat
    22741 attttacatt tgggttttca agacatccat cttctatcat aagttgagcc ccaccaatag
    22801 tttcctcggc aggttgaaaa aagaatttta catttacatt taagtaattc tttaatttaa
    22861 atagtatatt acaagtaccc agtaaaatag cagtgtgtgc atcatgacca catgcatgca
    22921 ttttaccaat attaatagat ttataagaat aattaagttc ttctgttata ggtaaggcat
    22981 ctatatcagc tcttatagca actgtttcaa agtttggact cacgttaatg tatgcagtta
    23041 tccctgtatg attcttatat tcaatataat ttatacctat ttcatttaag tatttaataa
    23101 tttttttctt agtttgaaat tcttctaatc caagttctgg agtcttatgt aaatctcttc
    23161 ttatatttat aatccaatca tttatctcat gacaaagagt tcttatatta agcataaaac
    23221 acatcctaac tatattaaaa atttattttt cttcattaat tatattattt tatgtaaatc
    23281 attattatgt atcaagtaca aataaaatat cttataatta tattagagat atttagtatg
    23341 gagaagaata tatcatttta taagaagagg tgtttaaatg ttaggaataa ttgcgccatc
    23401 tgggccatta agaaatacaa gtttggagga aataaagttt aatttagaaa gttatggtta
    23461 cgaagttaag ttttctgaaa gttgtagttt aaattataaa ggttatttag ctggtaatga
    23521 tgatattaga gcaagggata tagaagatat gtttttagat aaagatgtag atataattat
    23581 gtgcttaaga ggaggttatg gaacaacaag aatattggat aaaataaatt atgatataat
    23641 aaagcaaaat ccaaaaccat ttataggttt ttctgatata acaggtttaa atttagcttt
    23701 ttataaaaat tgtggacttt taccatatca tggaataatg gcagcaaatg taggtaaatg
    23761 ggataaattt acctataaat cattggtaaa tgctttagaa tttaaagatg acttatattt
    23821 agaaaatcca aaagaagaaa agatttatac tgtatgtgaa ggtaaagcag agggaataat
    23881 aatgggagga aatttatcct taataattgc cacaatggga acaaaatatg aaatagatgc
    23941 aaaagataaa attttgttta ttgaggaaat tggagaacct caatataaac tggatagaat
    24001 gttaacacaa ctatattctt caggaaaact tgaggaatgt aatggtataa tttttggaga
    24061 ttttaaggat tgtattgaag aaaatgattt gatggaatta ctaatagaat ttgcaaataa
    24121 ggtcaataaa ccttctatat ataatcttca gtcaggacat tgtatcccaa tgataactat
    24181 tcctttgggc agaatgtgcg aattagatgc tacagaaaaa atcgttaaat taaaaaaata
    24241 gtgtgtatat aaatataata gaaatatggt ataatcttat acaagtaaaa atttagctag
    24301 gaggatgaaa aatgtctgat gatagaataa tagactttaa cgaactaaag aacaaagtta
    24361 aagattctga tgtagataaa tttgagcaat acatatataa cttatatttt tctgttatgg
    24421 atggaaagat gtccatggct gagttttcaa gaaaaatatt cgattatatg agagataaca
    24481 acatatctca agaaaaattt atgaaaatac aaaagcaatt tatggagaga tatggtatgg
    24541 acactgaaga agtagaaaaa caacttagaa actttggaat cgacccttct acagcaggtt
    24601 ttatgagtaa taatacttcc agcaaagtat ctacagaaga tttagagtca tttaagaaga
    24661 gtgcaggatt ttatgagaaa tatggagaaa aaatccaacc aaagagttgt ataacaacat
    24721 ttataaaaaa tgacttaaat gatattaatg taataatcga ccaagaaaag ataatgcttt
    24781 gtagtgatag aaaaataaat cttatggatt cagaactaaa tgagttttta ttagaatata
    24841 agaatatgtt taataaaaaa ataaaggtag taatgtgcga aactacgaat aagtatgatt
    24901 attaaaaaat gaatttaaat actgaagtag tatcaattaa ttgtactact taaaaataaa
    24961 aatcgcaatt tattattcct tataaatttg tataaggaat atctaaactg cgatttttta
    25021 tattgtgaag attagtcata atagatagtg ttttaaattg taatcttctc actaaaactg
    25081 tgtttttcta ttagaaattt tattaaaaat aaatatctga taattgtatt atatgggatg
    25141 taaagtatta gaggcttaac gaatctcttt taatacatta actccatttt gaatcatact
    25201 gttgaaaaat actaagttta aaatctcagc agggtgaatg ttaggaatat cataagtatc
    25261 tactaaattc ataggtacaa ctatattttt ttcaatatta tgttgattaa aatatgattt
    25321 tagagttatt gcaaattgat atatgcatat atctgtgcaa tcgccaacta taattatatt
    25381 atctatatta tcaaaatcca aattttctaa tgcaaaaaat ccattagtag aatttttagg
    25441 aagaatttgt aggttttcta tcgacttaag ttcatcaacc aattcacttt ctacatcatt
    25501 ttccaaacag tgaacaggat aactcaaaag ttctattgat ttaggagtat gagaatcagt
    25561 aaatgctatt actctattta atttgtttga tatttttttt gtaaacattt caattggttt
    25621 tattaaagac tctactctag gagagtataa tgcaccttgt cttgcaaagc cattattaac
    25681 atcaattata aataaagcag ttttactcaa atcataattt tctatttttt ctattggtag
    25741 attatctaaa ttactcttta aagcttcaag ttcatttaat aagttatcca tagttatacc
    25801 tccaagataa atttaataaa attgtacata ttaattatat aaaagtcaag gagtacaaac
    25861 tattgtataa ttagctgtat tgttttgagt gtaaaagatt tgaagcaata tctggatagt
    25921 tagtaaaaat accatctaca ttgagtttta gacagtgtag tatatcatcc tcttcattta
    25981 cagtatatac attgacacca agtagatttt tgtgacaaag ttcaattaga gattcattta
    26041 ctaaaactag aggtggatga aggtaactat tacccagaaa tttagcaaaa ttgactacat
    26101 tgtttatagg taaataacac aataatcctg tttttacact agggtctaat ttatgaaagt
    26161 tttttaagct attgctgttg aaactagata taagaactct atttttcata ttatacttta
    26221 aaataagctc taaaacgtct ttttctatat tgggatagtt tattttgtca gtttttagtt
    26281 ctatatttaa aataaggtct gtaggtgaaa ttagaattaa tacttcttct aaagttggaa
    26341 ttttacattc tttattgttt tgatatcctt caaaagatga gtttagagtt tttaattttc
    26401 ttaaagtaaa gtcttttaca aaaccatgtc cattaaaagt tctatcaacc ttttcatcat
    26461 gaataactac aatttttcca tccttagttc tgtggacatc aagttctatt ccatatatat
    26521 ccatatacaa acatttttta aaagctagaa tagtattttc tggatacttt ccactaaacc
    26581 ccctatgagc ataaatattc atttatatgg ttccctccta taagtataac
//
LOCUS       DAELSY010000043        26609 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.043, whole genome shotgun sequence.
ACCESSION   DAELSY010000043 DAELSY010000000
VERSION     DAELSY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.043"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            273..1109
                     /locus_tag="KPG09_001594"
     CDS             273..1109
                     /locus_tag="KPG09_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0966212.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFKFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1462..3039
                     /locus_tag="KPG09_001595"
     CDS             1462..3039
                     /locus_tag="KPG09_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0966213.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            3032..4216
                     /locus_tag="KPG09_001596"
     CDS             3032..4216
                     /locus_tag="KPG09_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0966214.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVITNNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     TL"
     gene            4234..4833
                     /locus_tag="KPG09_001597"
     CDS             4234..4833
                     /locus_tag="KPG09_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0966215.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4857..5327
                     /locus_tag="KPG09_001598"
     CDS             4857..5327
                     /locus_tag="KPG09_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0966216.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIELKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
     gene            5684..6430
                     /locus_tag="KPG09_001599"
     CDS             5684..6430
                     /locus_tag="KPG09_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBG0966217.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            6492..7166
                     /locus_tag="KPG09_001600"
     CDS             6492..7166
                     /locus_tag="KPG09_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0966218.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCLNCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCALRCMMSCPWSL
                     RYANQK"
     gene            7534..8880
                     /locus_tag="KPG09_001601"
     CDS             7534..8880
                     /locus_tag="KPG09_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG0966219.1"
                     /translation="MEIETKTPDWINVLELNSKIDITWKYDVSNMIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIVGTTNFITRGVSNI
                     SIITLEGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLFHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     REIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITKCGQYYKSVDYNTYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLDEHKIYVIKANETNKVMLTC
                     YAEADELIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSIEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            8907..10307
                     /locus_tag="KPG09_001602"
     CDS             8907..10307
                     /locus_tag="KPG09_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG0966220.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSSKLQEIIDAAPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKIICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GEPGHYENLFFSGFSGIALDMPNNSTANDITVFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKVIKMHKPEVGDIMSTIIKDENILSAHYGGTWGKIGE
                     KIEFGITVHYYKLIEK"
     gene            10517..11953
                     /locus_tag="KPG09_001603"
     CDS             10517..11953
                     /locus_tag="KPG09_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG0966221.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPDLQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSPTETTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNEEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKDYEGYGSYKINNITIDQCGYCGFLFDSVKGSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSSPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGKETVYYYKHHEPDKTNQIK"
     gene            12297..13241
                     /locus_tag="KPG09_001604"
     CDS             12297..13241
                     /locus_tag="KPG09_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HBG0966222.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            13410..14342
                     /locus_tag="KPG09_001605"
     CDS             13410..14342
                     /locus_tag="KPG09_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBG0966223.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            14447..14914
                     /locus_tag="KPG09_001606"
     CDS             14447..14914
                     /locus_tag="KPG09_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBG0966224.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGETMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(15158..16012)
                     /locus_tag="KPG09_001607"
     CDS             complement(15158..16012)
                     /locus_tag="KPG09_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBG0966225.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            16178..18142
                     /locus_tag="KPG09_001608"
     CDS             16178..18142
                     /locus_tag="KPG09_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0966226.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLSEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYIGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVAADQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSCCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFVKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLLEESI"
     gene            18142..18603
                     /locus_tag="KPG09_001609"
     CDS             18142..18603
                     /locus_tag="KPG09_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0966227.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSDAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            18606..18926
                     /locus_tag="KPG09_001610"
     CDS             18606..18926
                     /locus_tag="KPG09_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG0966228.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            18942..20042
                     /locus_tag="KPG09_001611"
     CDS             18942..20042
                     /locus_tag="KPG09_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG0966229.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            20162..22762
                     /locus_tag="KPG09_001612"
     CDS             20162..22762
                     /locus_tag="KPG09_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBG0966230.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKHVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDYILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNVFIEESGYYILKVR
                     INVQLPALGYKVVSFESSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLILKDSGNAGDTYDFSPLKNDEDIKLSFDRVYTEKTLGYEKLVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPEKY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
     gene            23231..23566
                     /locus_tag="KPG09_001613"
     CDS             23231..23566
                     /locus_tag="KPG09_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0966231.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            23703..24980
                     /locus_tag="KPG09_001614"
     CDS             23703..24980
                     /locus_tag="KPG09_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBG0966232.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNNSQKDSNNDNNDNG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            25101..26489
                     /locus_tag="KPG09_001615"
     CDS             25101..26489
                     /locus_tag="KPG09_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0966233.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSESAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
BASE COUNT        10540 a         2708 c         4669 g         8692 t
ORIGIN      
        1 tactggaaaa aactgaaaat aatttttgta atataaaatg tcgattgatt ttaaataatt
       61 aaatatacat aagaaataat taaatatatt tcaatgaaaa tatgataaaa tatgacaaat
      121 taattgatga caataaaaaa tattttgtta ttgtagtatt atagggtaaa ttgcaaaata
      181 tatacgaaag taaaatttta aatattgaca ttaaattaaa tatactatac tatgtaagtg
      241 tgatgagaga atttttttag aggggattta tagtgaatat aaaaaagata tttaataaca
      301 atgtagtagt aagctcccta gaagatggca cagaaataat tgtcactggt gctggagtag
      361 gatttaagaa aaaagtaggc gatttgatag atgaaaattt gatttctaag aaatattttg
      421 ttcaagacga tcaaagggac aaatacaatc aaatacttaa taagacctct attgaatact
      481 ttaaaatttc agaagaaatt atagaaaagg ctaatgaagt tttaaatact caagtgaatg
      541 attcaataat attagcctta actagtcaca tagaatttgc tgtccaaaga gaaaaacaag
      601 gtataaaatt accaaactta atattgaatg agacaaaaca actttatagg gaagaattta
      661 aatttggatt atgggcaatt gatgaaattg aaaaaaggat aggaataaaa ttgcctgaag
      721 atgaagcagg atatattgct attcacataa tcaatggttt ggaaaattct caaaaagatg
      781 aaggtattaa tatactagag ttttcaaaac aagttattca gataattgaa gaagtacatg
      841 gatttaaatt ggatgtaaat tcattaaatt atactagact tataactcac ttaaagtttt
      901 ttgttcaaag gattctccga aaagaaactt acaatgatag tgatattgaa gatatgtata
      961 agttagtgaa taaaaattac aataaaccta aagtgtgtac tgaagaaatt gcagcattgg
     1021 tattagacag atttgaatat aagattagta aagaagaaga attatattta atgattcaca
     1081 taaataaaat aacaaacagt ggttgttagg attgtaactg ataaagcagg caaaacctaa
     1141 gtcaatgagc ttaactatta gattaagttt tttgatttag gtttttttat tagatacaat
     1201 caaaatacag acagtgactt ttaggattgt aactggttaa gcaggcaaaa cctaaatcat
     1261 tgaatttaac tattaggtta agttttttga tttaggtttt tttattagat acaatcaaat
     1321 acagacagtg acttttagga ttgtaactgg ttgagcaggc aaaacctaaa tcattaaact
     1381 taattattaa attaagtttt ttgatttagg tttttctata aatttgatta tgtttttaga
     1441 taaaaatagg aggggaaaat tatgaaaaaa aataagttag gtttatggaa cttcttccaa
     1501 atgttaggga aaacgtttat gttcccaata gcactactta gtgtttctgg gatgatgcta
     1561 ggacttgggg caggttttac agaccctaag atgattgaaa tggttccatt tctagggaat
     1621 gaatatgtta acttagtttt aaacttcatg ttaacaatag gattatttgc atttaacaac
     1681 ttgggagcat tatttgcaat ggcaattcca ctaggattat taaaaaaaga aaaagagttt
     1741 ggtgcttttt caggattagt aggatttata gcaatgcata taggtacaaa tttttattta
     1801 acaagagctg atttgttagt accagcagaa cagatgtcaa ctaatggtca agctatgatt
     1861 atgggtatac aaacatataa tacaagtgtt ttaggtggta ttgtagcagg attaattgtt
     1921 tatgcaatat atgataaagt tagtaattta aagataccag aatcattagg attctacagt
     1981 ggaccaagat tagttccaat tgttagttta atagttatga gtatagttgg attattaata
     2041 cctattgttt ggccaccatt tttcaatgca ttccaaagtt taggaagatg gatttcttca
     2101 gcaggaccat taggatattt ttcatatgca gtggcagaaa gagttacaat tccatttgga
     2161 ctaaatcatc ttgtaacatc tgtattccgt tttacaccaa taggtggaac tgctgttatt
     2221 gatggagaaa cgtactttgg tacattgaac atgtttatgg catatgtaga aaataatcag
     2281 attatacctc ttgatttagc tggtaaaatg gaacaaggta aattgatgat tcaatatgga
     2341 ttagctggtg ctgcattggc tatctaccgt acagcaaaac cagaaaatag aaaggcactt
     2401 aaaggacttt taatatcagg agtgttgaca gttattatag gtggtataag tgaaccaata
     2461 gagtttctat tcttattcat atcaccagca ttatttgctt tccatacatt tatgaatggt
     2521 ctagcaaaca tggtacttcc atacttaggc gtgttgatgg gattcacagg agatttaatt
     2581 gcatttataa gttttggtgt gttaagagga acagctacag gatggccaat agcagtatta
     2641 gtagcagttt tatattttgc aatatactat tttgtattta agtgggcaat tattaagttt
     2701 aatataaaga ccccaggacg tgaggataaa gaaataaaag tatctgaagg aaatggttca
     2761 gatttcaaaa atttgtctac atataaagga caacaaatga tatctgcttt aggtggtcaa
     2821 tctaatatat tatcactaga taattgtgta actagattgc gtttaaaact acaagatgta
     2881 tcattaataa atgaggatgc aattaaagag gctggtggta tagcagttgt taaattggat
     2941 gagcatacaa tacaagtaat tataggaact caagtgtatt ctttaagaaa gcagatggat
     3001 aaagctatgg aaggtcattt ggaggcatgt aatgattaat tttgataaaa aagtagaccg
     3061 attgggtaca tattgtaccc agtgggacta tgtagaagat agatttggta agaaggattt
     3121 gcttcctttt acaatttcag atatggattt agagtttcca tttgaaataa cagaggtgtt
     3181 gcaggaaagg ttaaatcata gagttttggg atacagtaga tggaaacatg atgattttag
     3241 aaattcagtt acgaattggt atattaaaag atttaattgt agcgttgata gtaactggat
     3301 atattatagt ccaagtgtaa tgtattcaat tagcaaactt attgagatgt taagtaatga
     3361 aggagatgga atccttataa atactcctgc atacaatgct ttttatgatg tgataacaaa
     3421 taacaatcgt aatattataa agtcacatct tgtaaatagg aatggattct attcaattga
     3481 ttttaatgat tttgagaaaa agtgtaaaaa atctaagata tttattttat gtaatcctca
     3541 caatccaact ggtagagtat ggagtgagtt agaacttgtt aagatgatag gaatttgtaa
     3601 gaataatgat gtcaagataa tatcagatga tatacacatg gatattgtat atgacaatag
     3661 aatgacacct atcttaaatg ttgcaaagga ctatttagat tctgtattca tttgtacatc
     3721 agcgtctaaa tctttcaata taccagcatt gactgggtca tatgtgatta taccaaatga
     3781 aggtattaga aataaatttg aaaatatcac tagataccgt gattttgtaa attcgccagc
     3841 aattctagca atattagcta caatggtctg ttatgatgac tgcgaatatt ggttagatga
     3901 actattaaaa taccttaaag agaatcttaa atatacgatt gattatataa aagataattt
     3961 agaaccattg aaattggtca tgccagaagg ctgttacttt gcttggatag atttttccga
     4021 acttaaaata agtggtgaga aattccaaaa attattgatg gatattggag aagttgctat
     4081 tatgtcgggg tctgtatatg gtgaagaagg tgagttttat ttaaggttaa atgtggcatg
     4141 ttcaagagaa aaacttgaag atggtttaaa taggattaaa aaaactatgg attatattgc
     4201 aaaaagaact ttatagtagg aagggagata gttatgggat atttaaaaac aatggttgat
     4261 gttatgacta ctgaaatgaa tgccataaga tgtttaatag atgaagcagg aatagaatat
     4321 gaaagtatag ttagtgaaat tgcaaattgt gaaggcaaag taatttttat gggtgtagga
     4381 aaatctgcac atataggtaa gaaattggca gctacatttg ctagtactgg tacacctagc
     4441 ttttttgtac atgcaacaga ggcagtgcat ggagatttag gaatgattga aagtaaggat
     4501 attacaattt taatatcaaa tagtggaaat tctatggaag tggtaaactg tataaaatat
     4561 attaaagcaa taggttctaa gacaattgca tttacttcaa acagaaattc agtactggca
     4621 aaagagtgtg attatgcttt aatatatcct gcaaaggatg aagcagacca tttaaatcta
     4681 gcaccaacaa catcatctac aattactttg gttttaggag attctattgc atgtgcatta
     4741 tctaagagta gtaattttgg ttcttcagat ttttataagt accatccagg aggaagttta
     4801 ggtgagaagt taaaaacagc taacaatgga taaaagcaaa aaggagagta gtttttatgt
     4861 tcaaaatgtt taaaaaaaaa tcaataaaat cacccatatc aggaaaagta atagagttat
     4921 ctaaaataca agatggtgta ttttctcaaa aaattatggg agagggtgta gctatagatt
     4981 ctacaggaga cattgtatat gctccaatca atggaaagat agtagtagta gcagagacta
     5041 aacatgcatt tgtaatagaa ttagaaaatg ggatggaact tcttattcat gttggtttag
     5101 atactgtaaa tttaaaaggc gaaggttttg aggcattggt gagtatggga gaacaagtga
     5161 aggaaggaac accaatgttg aaaattaacc gtcctttgat tgaattaaag ggtatatcac
     5221 ttattactcc agttacaata actaatcatt cagagtacaa tatgaataca tgtaatgtag
     5281 gcaatagcgt tgaaggtggg aaagatactg taattgagtt taaataaaga attaaaatac
     5341 aaggttaata tttataaata cctataaact tatgttttat aagattgatt gtatggattt
     5401 attgtataaa aaagaactct ttaattataa attaaggagt ttttttattt tgtaaatatt
     5461 gtttagattt atatagaaaa acacttagta ataaatagaa taaataagta tttatatagc
     5521 taaaaatcta aatagttttg tttaaatata tttttatatt ttgtaatttc aaattaataa
     5581 ataattagaa ttgataaaca aaaaataaaa atgttgatta tattaataaa ctttaatata
     5641 atatatttat gaaaaatgta aattgtgaaa tggaggtagt aacatgaaaa ttgaaaatct
     5701 ttctattgat gatattaaac gaggctatat atttaatgat gatttaaatt gttatagttg
     5761 cattaaatgt acaaaaacat atgaggtagg agaaatatat aaaataggta atagattctt
     5821 tgaagcttca aaagctattg aacttcatat ggaattagaa catagtgatt atttagagca
     5881 attgcttaat agtgattcaa agtacaacac attgactgaa aatcaaaaac aattatttca
     5941 attatttatt agtgatttac cagataagga gatagcaaaa caggttggag tatccacatc
     6001 tactatacgc catcaaaaat ttattttcag agaaaaagca aaacaggcta agctctattt
     6061 agcactatat gaatctgtat ttgaagataa atctaataaa agtagtacta taataccaat
     6121 ccatgaacat gctcgaatgg tagatgaaag atatgtagta actgaagaag agagaaaaca
     6181 cattttggat acatcatttg aaagtatgaa tcctttaaaa ttaaaagtat tttcttcaaa
     6241 agaaaagaaa aaggtcgtaa ttttatctaa gattttggaa caatttgaac gtgggaagaa
     6301 atattcagaa aaggaagtta atcagatttt agaatctata tatgatgatt ttgcaacaat
     6361 tagaagatat ttaattgagt atggctttat gagtagaaat aaagaatgta caaagtattg
     6421 gttaacataa ttaaaataat aaattttact aatgtgtaat gttattttta tctagggggg
     6481 aggagttaaa tatgaatgat gaattaaaat ctattttata caataaaggt gtagacattg
     6541 ttcgctttgt tgatatatct gaatttccaa taaatcaaac acaaggtttt tctaaagcaa
     6601 ttttattttg tatagggtta tcaaaaaaat ttattacaga tatatacaat aacttaccta
     6661 cagatagtga tgagtttttg gaaaaagagg agaaagtaga agaattagct gactggattt
     6721 caaaatatat tcaaaataaa ggatatagag cgtattctca atcggagaaa aacaatctag
     6781 aacatggata ttttgaaaaa gcctatatta atccagaaat gcaatcaggg attagtcctt
     6841 taccacataa gacaatagca aatattagtg gcattggatt tatgggaaaa aataatttgt
     6901 ttgtaacaga ggaatacgga tgtgctttta gcatgtgtac agtacttaca gatgctccaa
     6961 tttctgttga aagatatcct ttgattgatt caaaatgttt aaattgtaat gtttgtgtag
     7021 agaactgtcc tgcaaaagct atacatggaa atgaatggac tttacctgga aaaagagaat
     7081 ctattattga tgtttcaaag tgtttttgtg cattgaggtg tatgatgagt tgcccatggt
     7141 cattaagata tgcaaatcaa aaataacttt actagagaag gtattaaatt gaatattata
     7201 aaatttttaa aatgtaagat tatataagat ttctcaaaat aaaaatttgt atagaacact
     7261 cagaaatgag tgtttttcta ttgggattaa attatattga tacaaagctt aactatttta
     7321 atcattatat aaaatttgta ttaaaaaatt aagaataact tgctaatagt aaaaatataa
     7381 tgagattata attgatttat aatataactg taatgatgtt gtaaaatgaa aattgtcatc
     7441 tcaaaattcc caaaaagagt aaaagtcact tcggttatag tcaaaaaatt ttttctgtga
     7501 aaagaattta gatttaaaag ggaggaatta atcatggaaa ttgaaactaa aactccagat
     7561 tggattaatg tactagaatt aaacagtaaa atagatatta cttggaaata tgatgtaagt
     7621 aatatgatac aagatattgt ctctgataag tcactagaag gttccattat atattttcca
     7681 aaaggtaatt acttatttga gaaaggtatt aaaattagtc aacaaataac acttcaagga
     7741 gattcttatt atggaggaga taatcaagta tcaaatttag ataaaaaaca atcaatcgta
     7801 ggaacaacca attttataac ccgaggagtt tctaatataa gtattattac attggaagga
     7861 acaagtcaat gtatcaaaaa tattaatttt tattatgata gtcatgatat agagaaaata
     7921 ccacctaaaa atgtatctgc tattacagaa tatggagaaa cacaaggact atttcacttt
     7981 gaacacctgt ttatttctgg attttcagga atagggattg aaattccata ctattcaact
     8041 ggcaatgata tcattgttag ttcatgtggt cttggtatga ggcttggaga gaagtcaatg
     8101 ctttctagta gcaagattta tgaatgtaaa aatggaatgg agattactac tggtgttagc
     8161 ttaaataatg tgagaataga agaaataaga gaaataggta ttaataataa aggatttggt
     8221 tttaacctga ttatgaatct tatggtcgac caatgtggat attgtggatt tatgtttgaa
     8281 aaaatgtgtt atagccagat aactgccaga attactaaat gcggtcagta ttataaaagt
     8341 gttgattata atacttatga gaatatggaa gatagaaaag aagaagctta ttccattttt
     8401 tatggagata ttttagaaaa ttgcaatatt gtattgatga atagtaatat tgatacctta
     8461 gaaaatgatt cacttgatga acataagata tatgtaatca aggcaaatga aacaaacaaa
     8521 gtaatgttaa catgttatgc tgaagcagat gagcttatac agtgcgcaaa gggcaacttg
     8581 ttgctagaaa atggtgaaaa tacttataaa ttttataatg gtgagatttg ttctgtagga
     8641 ggtgttcaaa tttctgatat agatgaaaaa gatttaatca aaataatcga gaacactctg
     8701 tatatagatg ataaaggtaa gtacttaatg gttcctagat taaaaaagaa tactgtaata
     8761 tattcagtac tagattctat agaagaaata aataaattac atggtggaat atgggaacag
     8821 attgatgtaa aagtattagc tggggaatct atgtactatt ataaaaagat aaaagattaa
     8881 attatcaaac taggaggtag tgattaatgg aagagtctat aataattaaa gatataagtg
     8941 gctggattaa tgtacttgat tatgggattt gtaatgatga aacaaaagat gtaagttcca
     9001 agttgcaaga gattatagat gctgcaccag aagattctat tatatatttt ccaaaaggaa
     9061 aatacttatt tgaaagtggt gttaagatta agaaacgatt aaccttatgt ggagattctt
     9121 atattttggc gaatgttcct aaaaacactg ctggagtaac acaatttact tttaaaggaa
     9181 agaaaaatcc taaaagtgaa aatatagcaa ttataacagc aaaaagtgta agacactgta
     9241 ttaagatcat ttgcttttat tcagatagtt gtgatagaga tatgacatct aatcatgaac
     9301 ctccaacaga tggaaaacct agatttcatc ataatgtaat tataaattat agaaatatat
     9361 cggctattgt atgtgaagat aaacagggag aaccaggtca ttatgaaaac ttgtttttta
     9421 gtggtttttc tggaattgca cttgatatgc ctaataattc tacagcaaat gatattacag
     9481 tttttacatc cggacttgga attagtacag gggaaaattc aacgattgct aacagtaagg
     9541 tttggggctg tgttgatgga atgataattt ctactggaac ttttataaat ggtatgaggg
     9601 tagaagaaat aaaaaaagtt ggaattaaaa atattggtag aggtttaaat tttattacaa
     9661 atatcacaat tgaccaatgt ggatattgtg gagtttggtt tgattctata tccaatgttc
     9721 agatatcagg aaatatcaca aggtgcggac agtactatta tgatgtaagc tatgataatt
     9781 atttaatgtt ggataataga gttaaagaag catattcttt attctatggt aatttattgg
     9841 agtcaagtaa tatagttttg tcaaatacca ataaagataa ttgggaagat ttcaaagagg
     9901 ataagcataa ggtgtatctt gtagaagcac ttgaaaccaa gaatgtacta ttaacatgca
     9961 atatagatgc tactgactgg attgttaagt cagaaaaagg aaatttaacg tacaataata
    10021 tgcgacaaac tgttatattt tacgatggaa aactaagtag tatcaatgga attggtatat
    10081 ctgatgaaga ttatggggat aaagtcaaga taaaaaaagg aaatatatat ataaatagta
    10141 aggataaggt tattaagatg cataaaccag aggtaggtga cattatgtca acaattatta
    10201 aagatgaaaa tatactttcc gctcattatg ggggaacatg ggggaaaatt ggtgaaaaaa
    10261 tagagtttgg aataactgta cactattata aattgataga gaaataagta aatacgtagt
    10321 atttgaatag atgtatttta ttggagagct aggactatag ttttaatgta aatggtttgg
    10381 taatagcatg gattgaataa tataaaaata atataaaaaa ttaatttgat tatgtaaaat
    10441 tctatgaagt agtaaacctg tgactttagt catggtaggt tcaaattaag taaatgggaa
    10501 aggaatatat aatattatgg aaggaagaac tgggattaaa gatggaagat ggattaatgt
    10561 agtagaaaga ggtattaaaa atgatggttc gtacaacata ggtcctgatt tacaggatat
    10621 tattgatgaa atagaaacag atttctgtgt cttatatttt ccaaaaggaa aatacttatt
    10681 tacaagtggt gttaaaatta ataaagaaat tgtattacaa ggtgattcta attcacctac
    10741 agagactaca caatttataa ctcgtggagt agaaaatatg agtattatta cattgacagg
    10801 gaagaaacaa tgtattaaaa atataaattt ctattctgat agttgtgaga tacaggtaaa
    10861 tgaagaacca ccgacaaaag gtaatcctaa atatcattat gagatgatta ttaataagaa
    10921 tgaaaaaaat gttgagatga ataatgtatc agcaattctg tatgataata gtaagaatat
    10981 gattaaaggt ctaggtcatt atgaaaatat ttatataagt ggtttttcgg gtacaggaat
    11041 taggatacca tattactcaa ttgtaaatga tattacagtt tcatcttgtg ggttgggtat
    11101 tgatacagga atagacacaa ttatttctaa tagtagaata tctaagtgtc aaaatggaat
    11161 gaggattaga actggaacta gcataaataa tgtaagaatt gaaaaaatac agaaagttgg
    11221 tcttataagt agtcaagtta aggattatga gggatatgga agttataaaa ttaataatat
    11281 tacgattgac caatgtgggt attgtggttt tttatttgac tcagtgaaag gtagttattt
    11341 ttctggtatt ataagaagat gtgggcagta ttattataat acagattatg acacatattt
    11401 aagtattaaa gatagagtag aagaagcata ttctttattt tatggaaatt attttacaga
    11461 atgtaatatt gatttagtta gtgataataa ggataactgg gatgatggct tagaatataa
    11521 acataaagtt tatgtattta aaacccttca aataccaaaa ttaacattga gatgcaatat
    11581 agatgcctat gattatatcg ttaaatcggt acatggagga ggtaatttaa tgttaaaaaa
    11641 taactttaac aattatatat ttattgattc atctagtcca gaagttatta atggaattaa
    11701 ttttaattat caaaagaatg gaaacctgtt aaggttaaat aatggtaaat taagttataa
    11761 aaatggaaag gaagaaacag ctatattcag acctaattat ggtcttattg tagcatcaac
    11821 agattcagaa tgtgaacaaa tagagactag ctatggagga aaatgggaga agcttggttc
    11881 tgaagtaatt ggaaaagaaa cggtatatta ttataaacat catgaaccag ataaaacaaa
    11941 ccaaataaaa taaaatttgt attatataat gatataaagt tttaatatat agtgtttacc
    12001 tttaatgtaa tattggtata tttgtatagt attaaatttg ttaaaaagta aaattgtagg
    12061 gtgcttgaaa atagcacctt ttttagtttc attataaaat tagttttgaa agtttaggaa
    12121 taatgtatat ttatttttaa agaattataa ccttattaag gatattttaa atgctagaaa
    12181 tgaatcagta aatcatactg gaattatctt aaatgttttg attaaattaa gtatttagag
    12241 ctataattat attaaaaata tattaggaca taattatatt actatggagg ttaaatatga
    12301 tagtaagtgt gagtagaaga actgatatac ctgctttcta tagtaagtgg ttttttaaca
    12361 gacttaaaga aggttttgtt tatgtaatta atccaatgaa tccaaaacag gttagtaaga
    12421 tagagttaaa tccacatact gttgattgtt ttgtattttg gacaaaggat gtaactccaa
    12481 tgttatatga tttaaataaa ttaaaagact ataaatatta ttttcactat actataacat
    12541 catatggaaa agaagtagag acatgtattt tagataaaag aaaggtaata gattcattta
    12601 aggaattgtc tagaaaaata ggcaaagaaa gagtaatttt aagatacgac ccgattttct
    12661 taagtgaaaa gtacacagta gattatcata tcaaagcatt tgcgagttta tgtaatcagc
    12721 ttgatggatt tacagaaaaa tgtataataa gttttatcga tttatataag aaaacaaaat
    12781 ttaatactaa agcattacat ataaagccaa tagaaattac agaaatagaa acattatcaa
    12841 aagaaatttc taagattgct cataagcatg ggataatact tgaaatgtgt tctgaagaat
    12901 ataatttaag taaatttggg attggaaaaa gtaagtgtat tgatgataga ttaatatcaa
    12961 aaattatagg atgtgaagta gaggtaaaaa aagatagtac acaaagagat atttgtggat
    13021 gtgttaaaag tgtcgatata ggacaataca atacttgcag acattattgt ctgtattgtt
    13081 atgctaactt taattatgca aaagtagaag aaaactgtag gctttataaa gaagataata
    13141 aattactcat agatacttta agagatgatg caaaaataag cattagagat atgaagacta
    13201 ttaaagtaga taagaataaa caaattagta tatttgatta aaataaatat actaattacc
    13261 ttaatatatc aagaatatag atacttttga taacaaaatt aaacttaata gatattttct
    13321 atataagaaa gatggtcata atatgtcaat ttgaccatct ttttgatata attaaaacac
    13381 aaataattta gtaacaaagg aggcagaact tgaatttatt aaaaaaatat cctataccta
    13441 ttgcaagttt gattttgtca atttttactt taggtaactt attacaatct tatagtgaat
    13501 cattacgtaa tgtaattgga tttataggat ttattttgta tgcaatttat atagtcaaaa
    13561 taatattact tagaaagggt gttaaagagc aattagagaa ccctttaata tcaagtacat
    13621 ttccaactat aacaatggca acaatgatat ttgctacata tataaaacca ttatctttag
    13681 aattaagtat tatgatttgg agcataggaa ttgtgggtca tcttgtgtta atagttttat
    13741 ttagtaagaa gttcttagtt aaattctcaa taaaaacggt atttccatct tggtatatag
    13801 tatatgttgg gatagttgca gctagtgtta cggcaccagc atttaatcaa cttttgattg
    13861 gcaaaattgc tttttggata ggatttgtat cttatatagt tcttatacca gtaattttaa
    13921 agagagtatg gataatcaag gaaatgcctg aaccatcttt accaagtata gctatattag
    13981 ctgcaccagg gtcactatta ttgtctggat atattaattc tttttcagat aaaaataatg
    14041 tagtattata cttgttattc atattatcaa ttgtatttta tattatagtt ttgggatact
    14101 taccgaaatt attaagattg cctttttatc cgagttttgc tgcatttaca ttccctatgg
    14161 caataagtgc aatgggtatg aaactaatga atggttattt aactaaatca ggtcatttta
    14221 taagttggat ttcatatgct tctaagttag aagagataat tgctacagtg attattttat
    14281 atgttttagt tttatttatt atatatctta gtaaatctga aactacatct aaagctaaat
    14341 aagaattatt aaatatgtgt atttaatagg aatattgata taaatatatg aagataagat
    14401 aaaccataga aacttaattt agcttaattt tattggagga aaacaaatga aaacaaaaga
    14461 agaatattta aaagaagtta gaaacatatc agaagaatac tttagaaaag gagagttttt
    14521 ttgctctgaa gcagttcttc agacaataaa tgatgcttta ggccaacctt taagtccaga
    14581 gataactaaa ttagcatcag gatttccgat aggacttgga aaagctcaat gcctttgtgg
    14641 agcgatatct ggaggagaga tggctttagg tatagtatat ggaagagtac atggagaaac
    14701 tatgaatcca aagatgtttg agcatgctaa aggtttgcat gattatatta aaaaagagta
    14761 tggtgcaaca tgttgtagag ctattacaaa gaaatgggat ggtgataact ttatgtctcc
    14821 cgaaagaaaa gagcactgta ttaaaataac aggtcaagtt acagagtggg ttgcagataa
    14881 gcttatagaa gatgaaaaac ttaatatcaa atagtgtaat attagttggt gaatttataa
    14941 agttaaattt acttatagta taagatactt gatattctcg tattgataga aaaatttata
    15001 gcatatgtat ttaatgaaat atacttatat ttagagtgtg gtttaatttc ataaatacat
    15061 atgctattat tttaagtata taagcaaatt tatttaaata aatagaaaca gttaatttat
    15121 atgtacaaaa ctatatatga ttagtgtaaa taaataccta ttttataata ctcaacataa
    15181 agacaccaga catgattagt attataccaa taaaaatagc aagccattct tttttgttct
    15241 tcttttcttt aaaaaatata atccctccta aagtggctac tatcacacaa ctctgagaga
    15301 tagaaaaact tattgtaact cccaatgtat tagtcgttat gaacatacct aaatttgcta
    15361 ttgaccacaa tataccagta attaaattga ataaaacatt ttccctatat acaatctctt
    15421 tagaaaaata cttgacaata atagatgaag ttatcaacat accaacagat tgaggtaata
    15481 taattctata tccttcaatt aaaaatattt gattagttac aacatataat gataaaaata
    15541 gagatgataa taatacaact ccaacacatt ttaacatatt tttatctgta gattttttag
    15601 tttgataatt agtgagaagt ataccaagta ttatacataa tattgataaa gaaccaatca
    15661 gtaacatttt agaactggtc cattcattaa acaagattac agctaataat gtcgtaaaag
    15721 ttaattgtga accatttgaa attggcatta ctttagatac actagcaatt tgaattgctt
    15781 taaactgcat taattgtccc aaactccaga atatgccaga aatcatactt atacaaaatg
    15841 aaaaaaatgt aatttcagga tgtataacca gagttattat agtggaaaat ataaacgcac
    15901 ttatggatgt acctaaaagc tgttcatgag gagaactttt ctttagattt gcaactatgg
    15961 gcatcaatcc ccatcccatt acaggaagta aagcaataat tattgtcagc attaaactat
    16021 ttcctttcat attaaattta gacagctttt aatttatcat ctaaaattaa agtgttcaat
    16081 acataaaaat taccgtattt aatcaggaaa tattagtagt tttttagaaa aacattttcc
    16141 attaaaataa agataaaggg aaggagtaaa attgaaaatg ttaactgata tgattcaaaa
    16201 tcgtgaaatt aaaataataa agtttttatt aaaagaaggt catacaactg taaaagcaat
    16261 agcagatgat ttagatgttt cagaaaaaac agtttcaaaa tctcttaaag aagtagaagc
    16321 agtgcttaaa aagataggtc tttctttaat aagaaaacct aagataggtg tttatattga
    16381 tggagattta aaaaaaatac ttccttacct agataataaa ggaagtcaaa ttcctacaac
    16441 cagagaggag agagttatct atatatattc agtactttta aagagtaatg attatattac
    16501 tatacaacaa ctttctgatg aactatatat aagtcgtgga actattgaaa gggatttatc
    16561 agaagttgaa aaactgctaa aaaacgaagg gatttccata tataagaaac ctagtaaggg
    16621 aataaagtta aatataagtg aaagagataa aagacaactt actgctaagt ttatacataa
    16681 attttggagt agaaactggt atattaagca agagcaagaa agcttttatc aagcatttga
    16741 aaagatacag gcagatgtaa atggtatttt ttcggaagat gatttatcaa taattattga
    16801 tattcttagg aatttcagca gagaaagaaa atttgagttc tctgattatg aatttcagtc
    16861 attagttatt catttggcaa tagctgtaga gagaattaga aaaggcgaat atatagaaga
    16921 aagtttgtat attggtaata tagaaaataa tcaaatgtca aataccaatt atttagcaaa
    16981 acaattagaa gaaaaatttt caatattatt accaaaatct gaaattggat atataaattt
    17041 acatttagta gcggctgacc aacatagaga tactaatgaa ctaaaaaata cttcaactaa
    17101 tccttgtgaa acaagtacaa ataatatatc aaatcttcgg gaaattataa aaaatacatt
    17161 gtatgagaca gcttatgaca aggatttaat tgatggattg acttcacatg tacattcatc
    17221 aataaataga ctgaaatatg gacttagtat taaaaaccca tatgtaaata caatcaaaca
    17281 aaacttttct ttatcatttg agcaagcttt agatttaaag aaagtaattg aatttatata
    17341 tgatattaga ataaatgatg atgaatgtgc ttatatagca ttacattttg aagcttttag
    17401 agaaagaatg aaagaacttc cacaaaatat tagagtaatt ctagtatgca gtacaggatt
    17461 aggttcatct aggttattgt ctgcaagaat aaaaaaatac tttccaatga taaagattga
    17521 aaaagtactt tccgtacaag aattagtatc cagtaaaatt gatgcagatt tagtaataag
    17581 tactatttat ttagagttag aaacaattcc aaccatagta gttagcccta ttttaaatga
    17641 aagtgatata agaaccttgg aaagaaatat acaaaatgtt tcatgttgta cttctaagaa
    17701 gtataaatct ttcagaaacc taatctttga agaaaatata ttgttaaata ttgaagcttc
    17761 aaactatgaa gatgcaatta agtatataac agataaccta ataaaaaaag gttttgtaaa
    17821 agaaggtata gctaaaagtg caattgagag agaaaattta tcttacacgt cttttcagtc
    17881 aatagctaca cctcatgcta atcctagtta tattaaaaaa tctaatatat ctgttgcaac
    17941 gttaaaaaat cctattacat ggggaaatga aaaggtggaa gttattttct ttatttcatt
    18001 agaaaatgac aaaaacctag agcttgatga aatatatgaa gttttttatg actttattga
    18061 caataaaaaa aatataaatg acatattaag tgcaacttcc acaaaggaag tgtacgattt
    18121 gctattggag gagagtatat agtgaaaata tcagatgtat tgaaaaaaga gcaaattgtt
    18181 tttaatcttg atacaaatac aaaggaagaa acaataaaaa aattaagtag tatatttgtt
    18241 gaaaatggga ttgtggacaa tgaagaagaa tatgttaagt ctgttttgga aagagaagaa
    18301 cattcaacta caggtattgg taatggtatt gctattcctc atggaaaaag tgatgctgtc
    18361 aaaaaatctt ctataatatt tgcaaagact aagcataaga ttgagtggga tgctttagat
    18421 gaaaaaccag tagatagtat tttcttattg gctataagca atattgataa ggatagcaat
    18481 catcttgtac tattatctaa attagcaact aaacttatgg atgatgacaa tgtagatgca
    18541 ctaaaattag ccaattcaga acaagaaata atcaatatat ttagtgaaga aggagagatg
    18601 tagaaatgaa aagaaaaatt atagcagtta cagcatgtgc aacaggagtt gcacatacat
    18661 atatggcagc tcaagcgtta aagaaagctt caaaaaagat gggtcacctt ataaaagttg
    18721 aaacacaagg agctacagga atcgataatg agttaactac aaaggatgca caaattgcag
    18781 aagtagtaat atttgcagta gatactaaag tcagaaatgc tgaacgtttt gagggaaaag
    18841 aaatactaga agtaccagtt aatgcaccta taaaggatgc agagaaagta ataaatgatg
    18901 cattaaagtt aattgacact aaataaatgg agggaaatgt aatgttgaaa aagatagtct
    18961 ctgagttaaa aaagcatgtc ttaactggaa tctcatatat gattccatta gtaattgcag
    19021 gtgctatgat aatggcaata tctcgtgtag gtggttcttt ctataatata ccagatatat
    19081 gggatgctaa atacgcagaa agtgctagta gtattgttag attattacat gatctagatg
    19141 gatttggagg tactgcatta ggattaatgt ttcctgtaat tgctgcattt ataggatttt
    19201 ctatagctga taaattagct attgtaccag gtcttgttgg aggtatgatt gctaaagata
    19261 taggtgcagg atttttagga gctttggcag ttggtcttat agcaggatat acatgtttgt
    19321 ttattaaaaa tcatgttaaa ttaccaaaat cagcagcttc aatagtacca atatttatag
    19381 taccagtatt tggaacatta atcactgttg tattaatcaa ttatgtgata ggtatacctt
    19441 ttgccacatt aaatactgga ttagaaaatt ggttaaatgg attatctggt acaaatcaga
    19501 tattaatggc agctattata ggggctatga tggcagttga cttaggtgga ccagttaata
    19561 aagcagcttt aacaacatca cttgcattat taacaagtgg aatttatgca cctaatactg
    19621 ctgcaatggt aggaatagtt ataccaccac taggattggg acttgctact atactagcaa
    19681 aacaaaaata taataaacag ttacgtgaag ctgggaaatc atctcttata atgggtttaa
    19741 ttggagtaag tgaaggagca attccttttg cggtagaatc tccattaaaa gtaatacctt
    19801 catgtgtaat tggaacagca attgcttcag ctatggcagt tggtttaggt tcagtaaatg
    19861 caactccaat tagtggattt tatggatggt ttacagtaga gaattggcca atgtatgtgt
    19921 tatcaatagc agttggaaca gtaattgtag ctggattagt tatagtattg cgtggaaatc
    19981 cagtgattga ggatgatgaa tttgaagatg atgattttga tgaagcagag tgggaatctt
    20041 aaaatttaat aactatttaa gacgtgttat aagctatatt caatatgaat cttatactaa
    20101 ataaagtgtt aaatattcag tttagactta aatataaaag ataaagaaca gaggagaata
    20161 aatgataaaa gcacatattg ttaatcatac acactgggat agagaatggt attttacttc
    20221 tggagatgca ttagtactta gtgaacaact atttacagat gtaattgatg aattagaacg
    20281 taatccagat gtaagttttg tacttgatgg acaactttct attttggatg attatgtaca
    20341 attacacaaa gaaaaaatag aaatcattaa aaagttaata aaagaagata gactacatat
    20401 tggaccatgg tttactcaaa ctgatgcatt ctttgctaga ggagagtcta ttttaagaaa
    20461 cttaatgatt ggaatttttg aaagtaaaaa gtatggtaag tatatgaaaa ttgggtattt
    20521 gccagataca tttggattta atgcacagat gcctatacta ttaaagcatg taggtcttga
    20581 taatatattc ttttggagag gtatacatct tggtaaacat gttcaatcac catattttaa
    20641 gtggaaaagc ttaaatggag aaagttatat atatgcagta aatatgccac atggatatgg
    20701 aactggtatg cttttagagc cttcattaaa gtatgttaat ggacgcttag acccagctat
    20761 tgattttatt aaatcatata ctgatgtaga tgaggtgtta ataccttctg ggaatgacca
    20821 gttaaatata ataggtgatt tcaaaaataa aattaaagcg attaatgata tcggaaagta
    20881 ttcatatata acgagttcat atcaagagtt tttaaaatat ataaagtcca tagaatcact
    20941 agaaagctat agaggtgaat ttagagaacc tgtattagca agaatacata aatcaattgg
    21001 ttctagtaga atggatataa aattagcatg tgataaacta gaaaacaagc tgattaaaag
    21061 aatagagcca ttattagtaa ttgctaaaaa gagtaaaatt gaaataagca atcagttact
    21121 tataaataca tggaaaaaac ttttggaagg acaagcacac gatagtctag ctggatgtgt
    21181 aactgatact gtaacagatt atattcttca ccgtataaga gaagcaaatg aaatatgtga
    21241 cagtattgaa aatactattg taaaaaagat ttcagaaggg cttaaattat ctaaaaatga
    21301 tattttagta tttaatacag aagctaagcg ttttaatgga tataaagaga tacaagttgt
    21361 aagtgatagt aaaaatattt attttaaaga taatttagat gctactataa ttgaagaaac
    21421 ttatgtaaaa ccgagagaaa atgtattaga agaaacacct gctggcaacg tatttattga
    21481 agaatcagga tattatatat taaaagtaag aataaatgta cagcttcctg cacttggtta
    21541 taaagttgtt agttttgaat caagtgataa agaaatggta tcacttgata aatctaatga
    21601 cacttttata tctaatgata attataagat aatctatgaa gatggaagct taaatttaca
    21661 acttaaagat ggaacttaca tatcaaactt tttaatatta aaagatagtg gtaatgcagg
    21721 cgatacctat gacttttctc cattaaaaaa tgacgaggat ataaaacttt catttgatag
    21781 agtgtataca gaaaaaactt taggatatga aaagttggtg gttaaaggta ctactttact
    21841 accacttaca ttagaagata gaaaaaacaa atatcttaat gggaaattaa atgtaaaagt
    21901 aagtattgtc ttatccaaag aaaatccatt gatggatgta aaactttgtg ttgataacac
    21961 gatatataat catagactac gtgttcatat aaaaactgat ataaaagata ataaaaacat
    22021 agcatcattg ccatttggat atataacaag agaaaatgga gttttagaca actgggaaga
    22081 tatttacagt gaaatgccta tagatttaga acctttggaa agtaatatta ctctaacaaa
    22141 ccaaaataga agctgtactg tatttactag aggaataaaa gagtatcaac atattgaaga
    22201 tgaaattgca ttgaccttac ttgctactac agatgaacta ggaaagcctg atttattata
    22261 tagaccagga cgtgcttctg gtgatacaac taaaaaaggt catataagaa ttaaaacaga
    22321 taaagctcaa atgcttaaga aattagaatt ttcatttgca atatatatgg attctaaatc
    22381 atttgatgaa ttggaaattg cgaatttaac ccacaattat ctaagggaat gtgtaaatta
    22441 tcaattacag gattacaatt tctttttata tcgtatagat aataagattc aaaaatcaat
    22501 tgtcaataaa actgtaagta gagaattaga aataatatct ttaccagaaa aatatcttat
    22561 atcagcatgt tatccttctt attacagtaa agataaattt ataataagag tggaaaatcc
    22621 aagtagtaat agaatgatat tggatgagca aatatttaaa aatagaaatg ggaaaattgt
    22681 aaatgcaata gaagatgtag aagaagaaca ggtatatgaa atagcacctt ttgacgttat
    22741 ttcaatatta ttggatttat aagtgtaaaa gtaagtttat tgtttaaagt ctgacagata
    22801 ttaatctgcc agacttttta atttgtataa atttgggtaa atattaatga atagttttag
    22861 tatttaaata tgaatttagt atttaatata gaatttattg aagtagaata attagtttaa
    22921 caggataagc agtttaaata aagaattatt atattagagt gattttatat ataatttaaa
    22981 ataataaaat gttaataaaa tgagttaaag attgtatatt aatagggttg atgtagttac
    23041 aaaaaagtta ccaattgtta tgtatttaca aaactgatat attaattaca taaaatctaa
    23101 aatagtttgt aataggagta tatataataa aaattttaat taaataaagc taggtatatt
    23161 tataatatta ataaatatat tgctataaaa gtttgtttaa taaattaaat aaagaaaaga
    23221 ggagaataaa atgtacaaca tagatgatat gatatataat tgtccagtag aagctttatc
    23281 aaatatttta ggtaaaaaat gggtggctat aattatatgg aaaatacaag aagataagaa
    23341 acgatttgga gagctacaaa gagctatacc agactgtagt aagaaaatgt tagttcaaca
    23401 actagatttt ttaattgaac aggggattat agtcaaccaa aaaaagttgg ttaataatgt
    23461 tgtagagtca acatattttt taagtgaatc aggattacga ttgcttcctg tcatggaaaa
    23521 aatgattatg tggagcaata aaaatttaaa ttgtgataaa tactaaaatt tactctgtaa
    23581 gtaatgaaaa tttaattaag ctatgagtag aaaaatgtat taaaattaca taatttttta
    23641 gttagatttt aatttagaaa tgggattgta atattggaaa taaaataagg aggaatttta
    23701 atttgcaaaa tgtaaattta tttttagtat ttatagaagg tatagtatca ttcttttcac
    23761 catgtatact gccaattttg cctatttatt tgagtatatt gtcaaatagt agtgtagaaa
    23821 atctaaaaga aggaaagacg agttttatag gaagttcttt atttaaaaat actatatttt
    23881 ttgctcttgg aatctcaaca acattcttta tattgggttc atcagtaaaa gtattgagta
    23941 tgttttttaa tgaaaataaa gatttaataa tgtttattgg tgggattata attattatca
    24001 tgggattatt ttacatggga ataattaaat cttcaatatt gaatagagaa aaaagattta
    24061 atgttaagtt taaagaaatg aaagcaataa cagcttttat attaggtttt acgtttagtt
    24121 ttggatggac accttgtatt ggtccaatat tggcatctgt attagtcatg gtttcaagtt
    24181 caagtaatca tcttagtgcc aatcttttaa tagcagtata tactattggt tttattttgc
    24241 catttataat tacagctatg ttttacagta aactatttaa aactatagat aagataaaat
    24301 ctaacatgga aattattaaa aaaataggtg ggattatact tatagtatca ggaatattaa
    24361 tgatggtaaa tggatttgga agtataagca aacactttaa tacgtcacaa aatagcaaaa
    24421 ttgaaagcaa gcaagaagaa aataagcgag aaaattctac tgataaagaa gaaaattctg
    24481 atggtaataa tagtcaaaaa gactctaata atgacaataa tgataacggg tctaatgatg
    24541 aagatagaat aaagtctatt gattttactt taacagacca gtatggtaaa acacataaat
    24601 taagtgatta tgaaggcaaa gttgtattct taaatttctg ggctacatgg tgtcctccat
    24661 gtaaagaaga aatgccttat atagaacaac tgtataaaga ctataataaa aacaatgatg
    24721 atgtagtaat tttaggagta gcttcaccta atttaggtag agaaggttca agggaacatg
    24781 tagttaactt cttaaaagac caaggttata cattccctgt tgtacttgat gaagatggag
    24841 cattagcata tcaatatgga attaatgctt ttccaactac atttataatt gacaaggaag
    24901 gatatgtaac tcagtatatt ccaggagcta tggataaggc aactatggca tcatttattg
    24961 aaaatcagag aaataaataa aagtatttaa gtatatgttt atgaatcaat ttaaggttac
    25021 aaaaaagtgc ctaattgtac cacctaaata atcttgttat agtaaaagta taaacaatac
    25081 aagaaagggt tgaattttat atgaaaaaaa catttgatgc tataataata ggatttggaa
    25141 aaggtggaaa aacattagca ggagatttgg caaacagggg attaaaagta gctttaatag
    25201 aaaagtcaaa caagatgtat ggtgggacat gtgtaaatgt ggcatgtata ccaacaaaaa
    25261 gcttagaaaa ttcagcaaac agcgtaaaaa caaagaatat aaattcttgg gatgaagtac
    25321 aagctgaata tgagaaagct atagataaaa aagaaacttt aattacaaaa ctcagagaag
    25381 ctaactataa taaattaaat tcaaatgaga atgtaactat attcacagga atgggaactt
    25441 tcattgatga aaaaacagtt caagttaaaa ctgaaaatga aatttatgag ttagtagcag
    25501 ataatatatt tattaatact ggttcaagac catttatacc taatattaag ggaattgaaa
    25561 ataagaatat agtatatgat agtgagtctc ttatgaatct aagagtttta cctaaaaaaa
    25621 tgacaattat aggagctgga tttataggtt tagagtttgc tggtatttat agttcatttg
    25681 gagcagaagt tacaatatta aactctaata atggcatttt accaaatgaa gatgttgagg
    25741 attcagaaga aataataaaa ttacttgcaa agagaaatgt aaaaattgtt aataatgcaa
    25801 atataaaaga gattaaagaa gtttctgaat cagcaatagt agaatatgaa gtagatggta
    25861 aatctaaaga attaacaagt aatatgatac ttgtagctac aggaagaaaa gcaaacactg
    25921 aagggctagg gctagaaaat gcaggtatag aattaaatga aagaggattt attaaagtct
    25981 cagaaactct taagactaat aaagagcata tatgggctat aggagatata aatggaggtc
    26041 cacaatttac ttatatatca ttggatgatt atcgcatagt tattaatcag ttatttggag
    26101 ataagactag aacaactaat gatagaaaga atatcccaaa ttctatattt ataagtccag
    26161 cattttcaag agttggtctt aatgttaaac aagctaaaga aaagggatat gaagtattag
    26221 tggcaaagat gccagtagaa gcaattccaa gagctaaaca gattggcaag gcagatgggt
    26281 ttataaaaat agtgatagat aaaaaatcca ataagatact tggagctagt atgatatgtg
    26341 aaaattcaag tgagattatc catcttattc aattagctgt agatttagaa gtagagtata
    26401 catatttaag agaccgtgtt tatgcacatc caactatgac agaagctctt aatgatattc
    26461 tatctcctaa tatgataaaa gaagtataac ataagtttaa tatataaacc taaaattatc
    26521 attataagta gattttagaa agaggctatc tcaaaaaata gagataagtc tcttttttac
    26581 tttttaaatg ataaaaatat atttgattt
//
LOCUS       DAELSY010000044        26150 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.044, whole genome shotgun sequence.
ACCESSION   DAELSY010000044 DAELSY010000000
VERSION     DAELSY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26150
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.044"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(187..942)
                     /locus_tag="KPG09_001616"
     CDS             complement(187..942)
                     /locus_tag="KPG09_001616"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG0966234.1"
                     /translation="MLKVIINGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1383..2372
                     /locus_tag="KPG09_001617"
     CDS             1383..2372
                     /locus_tag="KPG09_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBG0966235.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMIVLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVINATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(2643..3359)
                     /gene="dapD"
                     /locus_tag="KPG09_001618"
     CDS             complement(2643..3359)
                     /gene="dapD"
                     /locus_tag="KPG09_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBG0966236.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLNEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(3583..4332)
                     /locus_tag="KPG09_001619"
     CDS             complement(3583..4332)
                     /locus_tag="KPG09_001619"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG0966237.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDNEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(4399..5286)
                     /locus_tag="KPG09_001620"
     CDS             complement(4399..5286)
                     /locus_tag="KPG09_001620"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG0966238.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(5403..6404)
                     /locus_tag="KPG09_001621"
     CDS             complement(5403..6404)
                     /locus_tag="KPG09_001621"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBG0966239.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(6829..7710)
                     /locus_tag="KPG09_001622"
     CDS             complement(6829..7710)
                     /locus_tag="KPG09_001622"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG0966240.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            8033..9226
                     /locus_tag="KPG09_001623"
     CDS             8033..9226
                     /locus_tag="KPG09_001623"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBG0966241.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            9432..10550
                     /locus_tag="KPG09_001624"
     CDS             9432..10550
                     /locus_tag="KPG09_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBG0966242.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            10681..10926
                     /locus_tag="KPG09_001625"
     CDS             10681..10926
                     /locus_tag="KPG09_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBG0966243.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            10913..11659
                     /locus_tag="KPG09_001626"
     CDS             10913..11659
                     /locus_tag="KPG09_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG0966244.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            11944..12819
                     /gene="hslO"
                     /locus_tag="KPG09_001627"
     CDS             11944..12819
                     /gene="hslO"
                     /locus_tag="KPG09_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBG0966245.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPDDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            13210..14010
                     /locus_tag="KPG09_001628"
     CDS             13210..14010
                     /locus_tag="KPG09_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG0966246.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            14537..16777
                     /locus_tag="KPG09_001629"
     CDS             14537..16777
                     /locus_tag="KPG09_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG0966247.1"
                     /translation="MSNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNDIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSRDGEISEATKKKLSKLLIEDRCPICDGRRLNEGVYRSLI
                     NGYNIADLTSMQIDELAEVIKSINEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHVVDVGPKAGKYGGKVVYEGSYKNLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEIKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQRKNIIEVLEMSVLDAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            17191..18726
                     /locus_tag="KPG09_001630"
     CDS             17191..18726
                     /locus_tag="KPG09_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG0966248.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTAMTISGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGIGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNISDLKNVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVIVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSEVFKMVGDILTDKEMAKMNYDVENNNLEPREVACEFLNKK
                     GLL"
     gene            18742..19503
                     /locus_tag="KPG09_001631"
     CDS             18742..19503
                     /locus_tag="KPG09_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0966249.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            19841..20709
                     /locus_tag="KPG09_001632"
                     /pseudo
     CDS             19841..20709
                     /locus_tag="KPG09_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            20903..21154
                     /locus_tag="KPG09_001633"
     CDS             20903..21154
                     /locus_tag="KPG09_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBG0966250.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            21266..22045
                     /locus_tag="KPG09_001634"
     CDS             21266..22045
                     /locus_tag="KPG09_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0966251.1"
                     /translation="MNNTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(22180..22830)
                     /locus_tag="KPG09_001635"
     CDS             complement(22180..22830)
                     /locus_tag="KPG09_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966252.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIAFPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIDHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(22827..23330)
                     /locus_tag="KPG09_001636"
     CDS             complement(22827..23330)
                     /locus_tag="KPG09_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0966253.1"
                     /translation="MKLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGRFGCWI
                     KTPSECVVKDYYSNMGKLINESDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(23379..23894)
                     /locus_tag="KPG09_001637"
     CDS             complement(23379..23894)
                     /locus_tag="KPG09_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG0966254.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKIDVEENHCIDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            24210..24701
                     /locus_tag="KPG09_001638"
     CDS             24210..24701
                     /locus_tag="KPG09_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0966255.1"
                     /translation="MRTIDIKGENMRELLKWVSIINRYNTNYIDRKLADIGINNSQYF
                     YVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGVKDKRTWHLYPTE
                     KSLECYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMNEEAKLEAKENG
                     RKI"
     gene            24685..26088
                     /locus_tag="KPG09_001639"
     CDS             24685..26088
                     /locus_tag="KPG09_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0966256.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
BASE COUNT         9681 a         3230 c         4325 g         8914 t
ORIGIN      
        1 gactataaaa taataaacaa tataattcca tataccaata aataagataa tctttaagaa
       61 aaatgattat atttaaaagg taagcataaa atttataaac ttttagaata gaaatctcaa
      121 tcaattagat attcaaattg atgaatatga atacctaaat agattgagat ttattatgta
      181 caaaacttat cctagtatat ctgacatttt ataaagtcca gattgcttac caattaaaaa
      241 tttagctgca actatagaac cgtttgcaaa tacttcatca gattgagaat gatggcttaa
      301 tgtaagaact tcattatcac ctgcaaatat gacatcatgg tcgctgatta tattgccacc
      361 tcttatagag tgtattccaa tttcatttgg ttttctttta caatctctac catatctacc
      421 atgtgtattt tcactttcag gcaagacttc tctaattgca ttagctatca tagtagcagt
      481 tccactaggg gcgtcttctt ttctattgtg atgtttttct atgatttcaa tatcaaaacc
      541 ttctaacatc tttgaagcat ccttaactaa ttttattaat atattcactc caagagatgt
      601 atttgatgat aataatatag gaatgttttt tgatgcttct tctattttat ttaagtcatt
      661 ttcatcatat ccagtagtag ctaatactac aggtgtatta ttttttagag catatgacaa
      721 tattggctct aaagtctcat gatgtgaaaa gtctattatg acatcagttt tttcagttac
      781 atcagtcatt gttttgtaaa gctttgtatc actatgatga cctttttcat cagcagacct
      841 agctacacca caaaccaact ctaattcatt atcttttttt acacattctg ctaaaactct
      901 tcccattgtt ccattatagc cattaattat aactttcaat ataaattcct cctatgaaat
      961 taataaaaat ttgttatatt tctataagtt tattttatca ctaatattat ttatataaaa
     1021 taatattagt gataataagc ttacaattta tcttacatta aaaaaattta agtggatttt
     1081 tcaaaatata gataaacttc tttttcattg tataaatgat aaaaatatat ttgacttcat
     1141 atcaaaaaga gtgccccatg aactaaaaag ttaattttga gataccatat tcatatatca
     1201 tatgatttat ttggaaatca atattaatac attaaaataa ctaaatacat gtatgcataa
     1261 agatgtataa aaaatatata aacatatagt attaatttta aattcaacaa tttaattgtt
     1321 aaaagtgtta ttattaaatt agaaatatct taacttaata tactgagatg ggagtgatag
     1381 atatgttaaa agaagaaaaa acttatcaag agaaagacgc aaaagtattc aatggagtgg
     1441 cagctttgat aatgattgta ttatttttag tagtttcaat aggtatcatg gtttttggga
     1501 tagtaaaact tgatagtgga tttactggaa taggttcact tatgttaatt ttgggacttg
     1561 tatttatagt agtagatttt atcctatttt ttgggctaaa aatgattaat cctaaagaag
     1621 ctatagttct agttttgttt ggtaactatt atggaactat aaaaaaagaa gggtactatt
     1681 gggtgaatcc attttgttca gcaataaacc cagcagtatc aagaattaat gtatcgaaat
     1741 cttcatctga tgaaaaggta gatatatcaa ctaattcaag gggcaaaaaa gtatctttaa
     1801 aaacaatgac tcttaataat gaaaagcaga aagtcaatga tttattggga aatccaatta
     1861 taataggagt ggttgtaatt tggaaggtta taaatgcaac aaaggcagta tttaatgtgg
     1921 ataattataa tacattctta tctattcagt gtgattctac tattcgtaat gtttcaagac
     1981 tttatccata tgatgtatct gaagatggag atgaaaaatc tcttcgtggt agtagtcagg
     2041 aaatagcaga tagattaaaa gatgaattac aatcaagggt tgacattgca ggaattgaag
     2101 tgtgtgaagt gagaattact catctttctt atgcaccaga gattgcagca gcaatgttac
     2161 aacgtcaaca agcagaggct ataatagcag cacgtaaaaa gattgttgag ggggctgtaa
     2221 gtatggtaga gatggcattg actaatttaa gtgaaaataa tgttgtgact ttggacgaag
     2281 aacgtaaagc agctatggta agtaatctgt tagttgtgct ttgtggcaat aaagatgcac
     2341 aaccagtagt aaacagcagt tctatttatt aaattataaa aagtttaata gtttattaca
     2401 ttgatatatt gaaaaattga aggaaataaa aatacttgtc atcaagaaat aaaaatgata
     2461 taagaatgaa taagagaatt gtaaaaaaat aaaatgatta taaataaaag ataaaaatat
     2521 tataaaaaag agggtgcctc tgatgaatta aattcataca gagacacctt tttaattaat
     2581 tttttaaatt agtatattat tgtttaatta atctttctaa ttaataatat agtactattt
     2641 aattagtcta aatttctaag gtcttccata agcttagttt tgtcagccgt tttttcatct
     2701 ttcattttta taactttagc aggagaacca gcaacaacgg caccagcttc tacatcagta
     2761 gttacaacag caccagctgc aacaacagca cctttaccga ttctaacacc ttcaagaata
     2821 actgcattag ctcctattaa aacatcatct tcaactataa caggagtagc agaaggtggc
     2881 tctaaaacac cagcaacaac agcaccagca cctaagtgaa catttttacc aagagttcct
     2941 ctagctccaa taacagcatt catatctacc atagaacctt caccaataac agctcctata
     3001 tttataacag ctcccatcat tacaacagca tttttttcta tagtaaccat gtctcttatt
     3061 attgcacctg gctcaattct tgcatgttca tttaaagtat tttttagagg aatagcagaa
     3121 tttcttctat catattctac atgagtatct gttatatcat tcccatgttt atctaatact
     3181 tccattatat cctcatagtc acctacaaat atataagaac cattacttcc aaatacctta
     3241 aatttatcag ttgattggaa gtgtaaagag ttagtattta catatacctt aacaggtgtt
     3301 ttctttttaa cttcttttat ataccttgct atttcataag catcgtttaa atttaccatt
     3361 tgttaattct ccttgtattt cattatattt cttttattat atacttattt gtcaatttta
     3421 gtatatactt acttcttatt atttatattt tattttagtt agtttttatt gtatagacat
     3481 agtattaaat aaatttatta tatatgtttg atttgtattg cgtattatta tatctatatt
     3541 gcatatgttt aatttatatt gtgtattatt atatttatac tattatccca acatgctgtc
     3601 catattataa aatcctggtt tttgcttaac taagtatttg gcagcagcta tagaaccact
     3661 tgcaaagata ccacgagatt gagcttgatg gcttattgta agaacctcat tgtctccagc
     3721 aaatagagca tcatgttcgc caacaatatt tccacctctt atagcatgaa taccaacttc
     3781 atttggttgt ctttttgcac tacgaccata tctaccataa ttattttcaa cgtttggcaa
     3841 aacctctttt atagcgttag caatcataac agctgtgcca cttggggcat ctacttttct
     3901 gttgtgatgt ttttctatta tttctatatc aaatccttct agtaatttag ctgcatcttt
     3961 aaccaatttt actaatacat ttacaccaag agacatatta tatgattgga atacaggaat
     4021 aactttagcc gcttcacgta ttttgttcat ttcgttatca ttatatccag tagtagcaat
     4081 tactaaaggt gttttagtct ttaaaacgta agataagata ttatctaaat tagagtgatg
     4141 agaaaaatct ataactacat cagcttcctc aactatagta ctcatatctt cataaatttt
     4201 aaggtcgcct tcatagctat ccagacctct agctgcaaca caaaccaatt ctaattcatt
     4261 atcttctctt atagtatcgg ctaaaatctt acccattttt ccatcataac cagttgctat
     4321 aacttttaac ataatttcct cctatgatta agggctaact tttgaccaaa cttacttttt
     4381 accatgcatt aatctttact atgcattaac ttttagtcca aagttaacta attctttctt
     4441 taacacttct aaatttgctg ggtccatttc tgctagaggt aatcttaaat ctccaacatt
     4501 gaatcccaat aaattcattg cagtcttaac tggtatagga tttacttcta taaataaagc
     4561 tgcgattaga gcatccatac caagttgtaa ttttctagaa ccttcaatat cgccttcaaa
     4621 gaatttagct accaaatcgt gagtttcttg agggcaaaca ttagcaagta ctgatataac
     4681 tccttgacct cctaaagata ataaaggaag tatagtatca tcattaccag agtagatagc
     4741 aaagtcatct ggaactaaac tagctatttc tgcaacttgt cctaggtctc cactagcttc
     4801 ttttacagca actatgttat caattttagc aagttcaact ataaggctag gacttatatt
     4861 tacacaagtt cttccaggga cattgtaaag tataattgga agtttaacac tgttagctat
     4921 tgtttcaaaa tgacgcttaa gtccagcttt attagcttta ttgtaataag gtgttattat
     4981 taatagagca tctactccta atttttcagc ttcttgactt aagttaactg agtgcatagt
     5041 atcattagaa ccacttccag ctataacagg aactctttta ttaacttttt caacaacata
     5101 cttaatagta gcaagttgtt cttcatcagt catagtatta gcttcaccag tagtaccaca
     5161 agatactata gcatcagtac cattagctat ttggtattca actaattcac ctaatttgtc
     5221 aaaatcaact ttgttatcct ttgtaaatgg tgtaactaga gcaacagcag aacctttaaa
     5281 tatcatataa aatcaatccc ctctttttta tttttgtgtt ttatattaca gttacttcta
     5341 atgcttgttt ttgagttact attaatattt tacaaaatta tatatttata caagtttgta
     5401 tattatacta aatcgtatga taaagccatt tcagctattt gaattgcgtt agttgcagca
     5461 ccttttctta tattgtcagc aacaacccaa agatttacac cattatcaac actaaaatct
     5521 cttcttattc ttccaacaaa tacttcatct ctatcatcaa attctacagc agttggatat
     5581 tcgtttttag caggattatc aactactaca acacctggag cttcttcaag tgtttttact
     5641 aaatcatcta actcaaatgg attgttaaac tcaacattta tagattcact atgaccattt
     5701 tttactggaa ctcttacagt agttgcagtt atttttaaat catagttatc aagtattttc
     5761 atagtttcat ttaccatttt catttcttct ttagtgtatc cattttcagt gaatgaatct
     5821 atatgaggaa gacagttgta agagatttga tgagggtaga atccagtagg attaccgttc
     5881 attcctttct ctaaatcttc aacaccttta acaccagaac cagatacagc ttggtaagta
     5941 gagtaaacaa ctctcttaat tttgtattta tcatcaagag ctttaagggg aaccatagct
     6001 tgtatagtag agcagttagg attagctatt atacccttat gattttttat ggcttctgga
     6061 ttaacctcag gaactactaa aggcacatct ggattcattc tccattgact agagttatct
     6121 actactaaga ttccatttga agcagctatt ggagcatact tttcactaat acttccacca
     6181 gcagagaata atgctatttg tatgcctcta tcagtaaatg aagtttcctt taattcttct
     6241 actatatatt ctttaccaca aaacattact ttagaaccag ctgatttaga agaagagaat
     6301 aaaaatagat tttctattgg aaaatttctt tcttctaata cttttaaaaa tgttctacct
     6361 accattccag tagcaccaac tattgctaca tttacttttt tcatataaat tcaccccaat
     6421 tttaaattat taaaacatta ttttatttta gttatacaag taagatatgt taatattttt
     6481 atatatccaa aaactttaat attatattaa caactttaat tatatacaaa aaacgtacaa
     6541 aggtatactt tgtacgtcta atttatcact caaagtaaac cctgtagctc atcacctaca
     6601 aaaataagtg acagtattac acatgttatg tgcaatccca gaaaatatta ttgcaagaaa
     6661 tattttcttc ggctgtaaat cctttttcca atcattaaca cctgccagct actattggat
     6721 actcttagtt acagctacct ctaccctagg ttaataggat attaaaattt gattattcta
     6781 ataataatac atatttaaga aaaaatcaac ttatttatat aaatattgct aaaacttaaa
     6841 tccaaaattt gtcaattctt gttttaacac attaagatta gtttcttcca tttcagctag
     6901 aggaagtctt aaatcaccaa cattaaaacc taaaatgttc atggcagttt taacaggaac
     6961 tggatttact tctataaata aagcagcaat taaagcatcc atatcaagtt gtaatttttt
     7021 tgagccttca atatcacctt caaagaattt agttactaaa tcatgtgttt ctttaggaca
     7081 tatgttagca agtacagaga taacaccact tccacctaat gataatagtg gtagtatagt
     7141 atcatcatta ccggagtata tagcaaagtc tttaggtact aatttagcta tttctgcaac
     7201 ttgagctaag tctccacttg cttccttcac agcaactata ttatctattt tagcaagttc
     7261 ggccacaaca gatggcttta tattcacttt agttcttcct ggtacgttgt ataaaataat
     7321 tggcaactta acgctatttg ctattgtttc aaagtgaagt ttaagacctt tctcattagt
     7381 cttattatag taaggagtta ttattaaaag accatccacc cctaattttt cagcttcttg
     7441 actcatatga acagacaaca tagtattgtt tgaaccagta cctgctataa caggtattct
     7501 cttattaact ttttcaactg tatattttat tacagcaaaa atttcagatt cactcatagt
     7561 agtcgcctca cctgttgttc cacaaacaac tagtgcatct gtgccattct caatgtgata
     7621 ttcgattaat tcacctaatt tttcaaagtt gacattatta tcctctgtaa atggggtaac
     7681 taaagcaaca gcagaacctt taaataacat ataatcaaat ctcctttttt atctaaattt
     7741 aattttttaa gtacaataat aattatagta caaattaatt aattttaata ggtatagtta
     7801 atatcttaac tgtttaaata agatatttat attatattaa tattagaaaa attttaaata
     7861 aatgttttat tctcaaaata tgcatattaa tttaataaaa tataccaaaa tggaaataaa
     7921 aaataaattt tatgagaatt taataactaa taattaatat atttaggtta taataaatat
     7981 gtgcgtaaaa tctaacaata agcaataata ccgtaaattt aggaggaata atatggatac
     8041 tttaaaggaa ctttttaaaa taggaagtgg accttcaagt tctcatacta tgggaccaca
     8101 aagagcagca gagagattta agaatgaaaa cccagatgca gaaagtttta gagcaatatt
     8161 atatggtagt ttggcagcta cagggaaagg tcacttaact gactatataa ttgaaaaaac
     8221 tatagcacct aaaaaagtcg aaattgtttg ggaagaagat attataaaag attttcaccc
     8281 aaatgggatg aagtttgaag ctttagataa agataaaaat gtaacagctg aatggactgt
     8341 atattctgta ggtggaggga ctatagcaga agaaggtcaa agaaacagta aaagcaatag
     8401 tatatatcac ttagatacta tggatgagat agtaaaatgg tgcaaagaaa ataataagac
     8461 tttggtagat tttgtgttgg aatgtgaacc aaaagacata aaagattata taaaaactat
     8521 aaaagatgct atgagaaaat ctattgatga tggactaagt acagatgaaa taataccagg
     8581 caagttatta ttaaaaagaa gagcaagtac attttataat gcatataaaa aagataagag
     8641 cttctcaaca cttgtatatg catatgcact agcagcttca gagcaaaacg cttctggtaa
     8701 tataatagtt acagcaccaa cttgtggttc tgctggagta ataccaggaa tattctttgc
     8761 aatgcaggat ttctataatt atgatgacga aaaaataatt gaagcactac ttgtagctgg
     8821 aataataggt aatataataa aaactaatgc ttcaatatct ggagcagagg tagggtgtca
     8881 aggtgaagtt ggagcagctt gttctatggc agcagcagca gtagcatact taaaaggtgg
     8941 aactatagac catattgaat atgcggcaga aatagcactt gaacatcatt taggaatgac
     9001 ttgtgaccct gtatatggat atgttcaaat tccatgtata gaaagaaatg caatggcagc
     9061 tcaaagagct tatgatgcag ctaattatgc attattaact gatggctcac attctgtctc
     9121 cttagaccaa gttgttgaaa caatgaaaga aactggtata gatatgatgg ataagtacaa
     9181 agaaacagct aagggtggac ttgctaaaca tttcttctct tgctaaaaat aaaattttcc
     9241 aataataatt cctctgtttt gatttatgaa acaggggaat tattattaat ggtgaattat
     9301 ttaataatgc aaaatatttt tatattcaat ttagcttttt atttttaatt agtataacat
     9361 gctatataat attttaatta taaagaaata tagaaatatt ttttaataaa ttagaaattg
     9421 gaggagatta catgagagag gcatcatatt tacaagaaca actttataaa cataggaagt
     9481 cattaaatcc tattgcagag attgggagaa aagagtataa gacttcaaaa tatataagag
     9541 attatcttga taaattagga attgagtatg aagtttattt agaaacaggt atagtaggtg
     9601 taatagaagg taaaagacct aaaaaagata ttgcttttag agctgacata gatggtcttc
     9661 atagtgaaga aggggtaaaa catttatgtg gtcatgatgg acatgctact atattacttg
     9721 gattaattga gtatttaaat gataataaag aagacttaaa tgataatata gtatttatat
     9781 tccaaccagc agaagaaggt cctgggggag caaaagcttt aatagatgaa ggagtgctta
     9841 gaaaatacaa tattgatgaa atatatgggc ttcatatata tccagagtta ccagaaggtt
     9901 atgtaggtac aagagaaggc tattttatgg ctcaaatagg agatatcgac atagaaatag
     9961 tgtctaaaag tggacatggg gctatgccac aaaatggtat tgatggtata gtgatagctt
    10021 caaactttgt aagctcactt caaactatag tgtctagaaa tataagccca atagacaatg
    10081 cagtgttgac tattggtaga attgaagggg gagctagaag aaatataata gctgaaaata
    10141 taaagatgga aggtactatg agatgtttca gcccagaagt gtatgagaaa atgaagctta
    10201 gagttagaga acttgctaga gggtttgaat tggcttataa ctgtaaggtt aatataaata
    10261 ttatagatga ttatattgct gttaaaaatg acaaggattt gtatcaagag tttgtagagg
    10321 ctattggaag tgatactgtt gtagagttag agccacttat gatatcagaa gatttttctt
    10381 attatcaaaa agaagttcca ggattatttt ttatgttggg ttcaagaaac gaagaaaaag
    10441 gatttgtaaa tggtcttcat aatataaact ttaattttga tgaaaagata tgtgtaaatg
    10501 cattgaatgt ttattcaaaa ttattaaaat acaaagaagc aatagattag gaagataaat
    10561 tataaagatt aaaaagcata aatacctaaa tttaaaaaaa atgtattaat aaatacccct
    10621 tatatatttg tataaattta ttttaattga caaaaataca aataagaagg ggtgattttt
    10681 ttgaaagaaa aagtacaaag agaaaagaag ataactaatg gcaagaatac taaaatattg
    10741 acaaatgatg atataatgaa atatgagata gcctcagaac taggtcttat ggataaagtt
    10801 ggtgaacgtg gatgggcagg acttacagct aaggaagcag gaagaattgg aggtatgctt
    10861 acatcacgca aaaagaggct taaaaagatt gtggaggagc agaataaaaa ggatgagtca
    10921 atatagtgat tttgcgtttg tatatgatga acttatgaat gaagttgatt ataatggttg
    10981 ggttaaatat atagaagata taatagatag tgaaggtgta aaagtaaaaa atatattgga
    11041 attggcatgt ggcacgggta atcttacaat tccattgact aaaaaaaatt acgatatagc
    11101 tggtatagat atatctgatg agatgcttag tgtagcaaga gaaaaggcag aaaaagaagg
    11161 agtagaatta gttttacttc aacaagatat atcagagctt gattttgaaa tatctgattt
    11221 ggactgtgtg ctttgtgctt gtgatggatt taattatata acttatgatg atgatttgga
    11281 aaatgtattt tcaaagacat atgaattatt aaagaaagat ggaatattca tatttgatat
    11341 aagttctttt tataaattag ccaacatatt gggaaataac atgtatggag aaaatagaga
    11401 agatatagcg tatatgtggc aaaattactt tgatgaagaa gagaatctaa ttgaaatgga
    11461 acttacattc tttattaaag atgaatgtgg aaagtttgaa agatttgaag agatacacca
    11521 acaaagagca tatactgaag atgagatagt tgatatgctt gaaaaatctg gatttaagga
    11581 tataaaatat tatggggatt ttacatttga caaacccagt gaagaaagtc aaagaatctt
    11641 ctttgtatgt aaaaaataag atatttttga ctatataaga taattattaa aaataaccat
    11701 gatataaaaa tatagaaaat gcagttttat agattaaaag taaagtgatg atactttatg
    11761 acttttattt tctaataatt ataaagttct atagataaaa attaaaatat aataactatt
    11821 gcttagtaaa attaaaataa aatgggtttt gcccaaggtt aatatagata agtacttata
    11881 gattaaaatt aaggtagtaa tataataaaa ataagacaat taacgggaca tggaggaata
    11941 ttaatgagag attatgtact aagagcgaca tcaggaaatg gtcaagttag agcatttgtc
    12001 gcaactacta gaaatacagt agaagaagct agaagattac atgaaactac aaaagtagca
    12061 acagcagcac ttggaagaac attaacagcc acatctataa tgggattaat gatgaaaaat
    12121 gatagtgata aattgactgt aataataaaa ggtggaggtc caattggaac tattatcgcc
    12181 acatcagatt ctaagggaat ggtaaaagga tatgtaggaa atccacaagt agaggttgaa
    12241 gactatccaa atggaaagtt aaatgtagca gcagcagtag gtacagaagg tgttgtaaaa
    12301 gttataaaag acttaggtct tagagaacca tataatggga cttatccatt ggtaagtgga
    12361 gaaattgcag aagactttac ttattatttt gctgtatcag agcagactcc atcagtagta
    12421 gctttaggtg ttcttacaaa agaagatgaa gtagagtttg ctggaggatt tatagttcaa
    12481 ttaatgccag atgcagaaga agagactatt gctaagttag aggaaaatgt agctaaatta
    12541 ccttctataa ctaacatgtt aaaagaagga aaatcaccag atgatatatt aaatattgtt
    12601 ttggatggtt tggaacctaa aatacttgat acctgtgaag tagggtttat gtgtgaatgt
    12661 tcaaaagaaa gagtaaaaac tgctttggtt gcaattggta aaaaatcatt ggctcagata
    12721 atagaagaag ataaaaaagc agaagttggt tgccagttct gtaataagaa gtatatgtat
    12781 agtgaagaag agttgcttga aattttaaag gaaatgtagg ctataaaaat agttgtattg
    12841 atataagtaa tgttatagtg atatagtaaa gtcgtattga tatagtaatt aaagtataat
    12901 ttattaataa tagacatatt tatattaaaa aataaattta ataaattttt aagcttaata
    12961 aattttaaga attaagaagc tttctggctg taatatttgc aatgttgcag ttagaaggct
    13021 tttttatttt attatagttc gtgtcaaaga ttgtatgaaa caataaaata ttgtaaaata
    13081 atttgtatat ttagacctga ttgacacaaa ggtttataag atatttgagt tgattatacg
    13141 aaattctatg aagtagtaac cctgtgactt taatcatggg aggttcagaa gaaggagaaa
    13201 attttaaata tggaaagtaa aacgaagtac ataggaataa gtatattaat attattgatg
    13261 atatttttaa ttgcaaagaa ttttaacgga aaacttgaaa tacctattcc tgagagtaat
    13321 agtagaaaat ttaaatcagc agcagtgttt tatcctcaac atcaagatga tgaggtttta
    13381 tggggaggaa gtgccattgt agatgctata aaacagtgtg gtgttgataa tgtatatgtg
    13441 gtattagtat cagatggttc tggagttaac gttttcaaag ctaacacaaa atttagaaac
    13501 ttaactagga agcaaaagga agagcttaga aataatgagt ttaaatcagc tttgagagag
    13561 cttggagtga aacctcaaaa tgtgattata cttgcagata tagataaaaa ggaaggcaca
    13621 cattatgaat taatggaaaa gactatattg gattttgaac ataagtttaa aggcaatgtg
    13681 acccatatag cccatcacta taagtatgat gaccatataa tgcatagaaa gaatggggaa
    13741 gttttaaaga aacttaaacg tgaaggtaag ataaaagatg acttgtattt tgttaagcca
    13801 aaatatgtaa agtatatacc aagaaatgaa agagctatct ataaagcaat ggattttgat
    13861 gactacaata aggttaagag tgcttgttat gaatataagg ttgtaaattc aaagcgtaaa
    13921 atgtatggta taggatatat ttcggcacat agttattttg aacatcttct aaatgaccct
    13981 gaacttacgt ctgtattaag tgaagattaa tttcttataa gtagtaagtt atgtttgata
    14041 agttgattgg aattttctat agttttgaag gtattggaaa tttagtttta tttaaagatt
    14101 taaagataga ggtttttaat gtttaaaatt ttaaagttaa gttgaataat tgtatagaat
    14161 tatttgtgaa agaagaaaca gtgtaaaaca tagtatctta tttatggttt taaaatcata
    14221 ggcaagatac tttttttatg taactaatac cattttgagg ttattaactt aagaatttta
    14281 tataatttaa cactcatata taatgatgat atttaggtag aatagatgca attacgctta
    14341 aataaaataa ctgtattttt tataaaaatc aaaccctaca ctatactacc agataaaatc
    14401 tacatgctat aatttgacct gtgtttataa aaaaaggagt aatctaataa aatagtattt
    14461 cacttatgta taactaacaa ctattattac gattttctct aaataattta aagaaaaata
    14521 atgcaggagg taaaatatga gcaatgaatt tataatttta aaaggttgca aagagaataa
    14581 tttgaacaat atttccttaa aaataccaaa aagaaaaata accatattca caggggtgtc
    14641 gggttctgga aagtcatcta ttgtatttga gacaattgca aaagaatcac aacgccaact
    14701 aaatgaaagg tttagtacat ttgtaagaag ttttttacct aagtatggag aggttaaagc
    14761 tgattgtata gaaaatctgt caacaccaat tatcatagac caaagtagac ttggtggaaa
    14821 ttcaaggtca accctaggta ctattacaga tgtaaattcc tttttaagag ctttatattc
    14881 aaggtttggt agtgagtata taggtaatgc taatatgttt tcttttaatg atattgatgg
    14941 tatgtgccca cactgtcatg ggcttggaaa aaagctagtt cctaatatga accaaattct
    15001 tgatatgaat aaatcattaa atgaaggtgc tatattgctt tcagggtttg gagtgggttc
    15061 ttggcattgg aatatattcg ctgaatcagg attttttgat attaataaga ggatatgtga
    15121 ttattcagaa gtagaattgg aaaagttttt gcatggtaaa gcagaaaaga ttaagataga
    15181 aaatgctggt caaactaaca tgacatatga gggattaatg gttaaattta atcgtctata
    15241 cttaagtaga gatggagaaa tatctgaggc tactaagaaa aaattgagta aacttcttat
    15301 agaagataga tgtcctatat gtgatggaag aagacttaat gaaggagtat ataggtcttt
    15361 aattaatggt tataatatag cagatttaac tagtatgcaa attgatgaat tggctgaagt
    15421 tattaaaagt ataaatgagc cagaagcaca acctttaatt aaaggaataa tagaaaagtt
    15481 aaacagtatc atagatatag gtcttggata tttaacactg gatagagaga catcttctct
    15541 atcaggaggg gaatctcagc gtataaagat ggttaagtat ttaaatagta atcttgttga
    15601 tttaatgtat atttttgatg aaccaagtgt aggacttcat ccaaaagatg tgtataaatt
    15661 aaataatctt ttaaaaaaat tgcgtgataa agggaataca attatagttg ttgaacatga
    15721 cccagatgtt attaagattg cagaccatgt tgtagatgta ggaccaaagg caggtaagta
    15781 tggtggaaaa gttgtgtatg aaggcagtta taaaaatcta cttacaagtg gtactttgac
    15841 agggaatgct ttaagtaaat ttcttcctat taaagaaagt gtaagagaac atagtggata
    15901 tttagaaatt aaaaattgta ataaaaacaa tttaaaaaat gtatctatta agatacctaa
    15961 aggtgtattg actctagtta ctggagtagc aggagcaggt aaaagtacgt tgattaagga
    16021 tgaattttta aagcaaaatc ctagtgctgt acttatagac caaagccctg tttcagcaaa
    16081 ttcaaggtcg agtttggcaa catatagtgg aattatgaac aacataagaa gcatattttc
    16141 aaaaacaaat ggcgtaaatg cttctttatt cagttcaaat tcagaaggag cttgtgataa
    16201 ctgtaagggt tctggaatag tagaaatgaa tcttgctttt atggagagta taaagagtac
    16261 ctgcaatgtt tgtgaaggta aaaaatttaa aaaagaagtt ttagaatata ggtttcaaag
    16321 gaagaatata attgaagttc ttgaaatgtc tgttttagac gcaattgagt tttttaattt
    16381 aaaacagatt aaaacgaaat tacaatgtat tgaaaaaatg ggtattggat accttacttt
    16441 aggacaaact cttgatactt tgtctggagg agagtgtcaa agattaaagc ttgctagtga
    16501 attaaataag gaaagttcag tgtatatatt agatgaacca acaacaggtc ttcatatggc
    16561 tgatattgag aattttataa gtataattga agagattgta gataatggaa atacggtcat
    16621 aataattgaa cacaatatag atgtaattaa aagagctgat tggattattg agctgggacc
    16681 tgaaggaggg acaaaaggcg ggagagtaat atttgaaggt atcccaaaac aattatgtaa
    16741 caataaaata tcattaacag caaaatatat agtataataa ataaaatata ataaaattat
    16801 aatgatattt taataaatat aatgggtata taaaatgata attaaactta acagtaataa
    16861 ataatgtgag aaatattcaa atttaattaa ataaagtgtc agaaatttgt ttaaatttta
    16921 gtattttaac gattctgacc ttttttctta tttttatgga gagtatcgaa gatggagtct
    16981 ctaatagtta cttttttgtg tctacttgca agtttgatta aaaaatggct atacacactt
    17041 gtgacttcag tcatgggagg ttcagattat aatcttaata tataatttgt gaaataattt
    17101 gatgacgatt tgctgatgta taaacaataa agattaacta cattaaaatt taaatcttgt
    17161 taagtaaaac ttataaaagg agagagatgg atgataaatg atattctaac cttattatct
    17221 gaaagaagtg atttttttat aaagctatcc atagaacatt tgatgatttc tattatttct
    17281 ataataattg cgattgtttt gggtggtata attggtatta ttgtaagtga atttcataat
    17341 tcttctaaac ctatattggg aactatcaat tttctataca ccattccatc tatttctatg
    17401 ctaggatttc tgataccatt ttccggaatt ggaaatacta ctgctgttat tgcacttacc
    17461 atttatgcat tattgccaat ggttagaaat acatatactg gaattactaa tgtggataaa
    17521 aatattcttg aagcagctag gggaatgggc agtacagatt ttcagatatt gtataaaata
    17581 aaacttccat tagccctacc tgttattatg tctggtatta gaaatatggt gattatgaca
    17641 attgcattag ctggtatcgc ttcatttata ggtgctggag gcctaggtgt agccatatat
    17701 agaggaatca cgacaaataa cacagcaatg acaattagtg gaagtatact tattgctcta
    17761 ttagctttaa tatttgattt tattttaggg ctggtagaaa aattttttca acgaaaaaga
    17821 aaaaagaaaa ataggaaaaa agtttttgcc attctaggaa gccttattct tatagcaact
    17881 atctttacgt cttatttatt taatccttca aagactcaaa ctattcatat tgctactaaa
    17941 cctatgactg aacaatatat tcttggtgag atgttggata ttttaattga aaaaaacaca
    18001 ggtctaaacg tggaaattac acaaggcata ggagggggaa catctaatat ccagccagct
    18061 atggagaatg gagaatttga catttatcca gagtatacag gtacaggctg gaatatggta
    18121 cttaagaaaa atgaattata tactgaaagt atgtttagta aactacagtc ggaatacaaa
    18181 actaaactta aaatgcaatg gactggtatg tatggttttt ctaatagcta tgcattagca
    18241 gttagaaaag aagttgcaga taaatataat ttaaaaaata tatcagactt aaaaaatgta
    18301 tcttctaaat tgacatttgg agctgaatat gacttttatg agagagagga tggatatact
    18361 ccattgtgta atacatatgg actgaacttt aaagatacag ttgatttaga tattggatta
    18421 aaataccaag ctatcaatga aggtaagatt gacgtaatgc ctatatttac aactgatggt
    18481 cagtatagtg tttcagatgt tatagttcta aaagatgata aaaatttcta cccatcttat
    18541 caatgtggaa atgttgttcg tttggaagta ttagaggaaa atccagaact atcagaggta
    18601 tttaagatgg ttggagatat tttgactgat aaagaaatgg ctaaaatgaa ttatgacgta
    18661 gaaaacaaca atttagaacc tagagaagtt gcttgtgagt ttctgaataa gaaaggtctt
    18721 ttataaattg gaggaaatat aatgacacct attatacaat ttaaaaatat taaaaaacaa
    18781 tataatgata aaactataat agataatctt aatttagata tagaaaaagg agagtttttg
    18841 actgtaattg gttcgtctgg ttctggtaag acaacactct taaaaatgat taatggtctg
    18901 attttgccag atggaggaaa tattttgata aaccaaacag atatcaaaaa cgaggactta
    18961 attaaattaa gaagaagaat aggatattgt gtacaaggaa gtgttctttt tcctcatatg
    19021 actgttgaag aaaatatttc atatgtacca aatctcctaa gtaaaaagaa taaattagaa
    19081 gtaaaaagtg ctgtaaacaa gtggatggaa atagtgggat tgccaaatga tatgaaagtg
    19141 cgttacccat cagaattgtc aggtggtcag caacaacgtg tagggattgc aagagcttta
    19201 gcttcttctc cagaaatttt attaatggat gaaccttttg gtgcagttga tgagattaca
    19261 cgaaagcaat tacaaaaaga gataaaagaa attcataaaa aaacaggagt tacgattatc
    19321 tttattacgc atgatattta tgaagctttg attttgggaa caaagacttt ggtattaaat
    19381 catggagttg ttcaacagta tgatactcct gagaatattt taaatactcc tgctaaccaa
    19441 tttgtagacc agttattgaa tataagaaaa tctataatag atgatgataa tttaaaagaa
    19501 taaatttaaa tttaaaatca aattgttgtg ataaaaacta ttgatttaga gctaacttca
    19561 agttgtatac ttgaagtgga aaagttagat taaggtatag aagagaattt agcagttggc
    19621 tgtagacgag gtaactgctg aaggtgcttt caataaatgg ctagaacctg tatctattga
    19681 agattataat aaattgaaat aagaagctca atatacagag tagataggca cttcaaatag
    19741 aagtgtcttt tgtgtgccat aaaataaatc aaaatattag caaagaatgt gaaaaacaaa
    19801 ataaaataat atgctatctt taaaatagga ggggtgctat atggataaca atttgattac
    19861 cattgaaaaa actttagatt atatagaaaa acatctttta gatgagatgt tagatttaaa
    19921 tgtaatttct aaagagatag gctattcaaa gtaccattta catagaatgt ttacatcagt
    19981 tgtcggattt agtatacata attatataca aagacgtaga ctaactgaag cagcaagaat
    20041 gctcattttt acagaaaaac caattattga aattgcttta atctcggggt atgacacaca
    20101 acgttctttt tcaacagggt ttaaaaaaat gtttaagcat agccctaagt tttttagaaa
    20161 acatcaagat tttttaccac tacaattaaa atttgatatt aataatagaa aaaggttaca
    20221 tggaggtatg gttttagatg ttaaaaaagt taattgtaca gatataaata ttgttggcta
    20281 ttctggtaaa gtaaaaaaag gatttcatgt tattggaaaa tgttggaggg atttgcataa
    20341 aaataaagag ttgatagaaa acaaggtaga cttggatttt ttaattggag ttaatgatta
    20401 tagtaatttt aaagaaattg aaaataatcc tacatttaca tatattgcag gtgttcaagt
    20461 agctactttt ggtaaattcc taatggaatg gaaaaattcg tattaccgaa aagtaattat
    20521 attgttttta cttttattgg gaaaaatgag gatagtatgc aaaatgttgt agaatatatc
    20581 tatcaagaat ggttccttaa ttcaagttgc cagtttaatg aaaataattt atatgatttt
    20641 gtaaaatacg gagaaaaaac tgataagaat ggtttgagtg aaattcagtt ttgggttcca
    20701 atcatttaaa agaagggatt atgtataaag taggtggatt tatataggca aagttttata
    20761 aaagtatgta tttataaata atttatagtc tgtaggaaag aaatagtttt actataagat
    20821 tatgataaaa tacacaatac tatgataaaa tagtactaaa taatagattt aattttgcaa
    20881 aggataaagt gttttaataa aaatgaatgt agaaaacgaa ttacttaaaa acctagatag
    20941 attgcataca acagagttag gcgttgtaag aattaagaaa aatctttctt tggagacaaa
    21001 tgatgtagtg aactggtgta aaactaaaat agaatctcca aatgccatta ttaatagaaa
    21061 gggaaaaaac tggtatataa gtgtatatga ttgtattata acagtaaacg ctcacagtta
    21121 tacaattatt acagctcata aagaaaagaa atgaattgaa aaatacaaag gtcaaaagca
    21181 ttttccactg tttgtagtac ctatgaagaa gtatataaaa taatattact tttgaataat
    21241 aatttaaatt ttggaggttg aaaaaatgaa taatactgta aaagaaaaat tggcagagtc
    21301 actaacttct gatactactg atattttacc ttatttgcct tatttattac aagatttttg
    21361 ggaattagga agttcaccag aacaaatgtt acaattattt agaaagcata taccttttaa
    21421 tgaggataca aaagttttgg atttagcttg tggaaaaggt gctgtatctg tgaagcttgc
    21481 aaaagagttg aatatacata tcaaaggtat tgatttgata cctgaattta ttgattatgc
    21541 gagaagaaag gctattgaat ataaggttga tgctttgtgt gattttgaaa tgaatgatgc
    21601 taatagttct gtaaaaattg agcacgatta tgattgtgtg atttttggag ctgtagggga
    21661 tattttaggt aattataaag aaactataag taagctatta cctacaataa aaagtaaggg
    21721 gttcatttta attgatgatg catatgtagt agataaatct acaaatgaac agcttaattt
    21781 tgagagggtg tatcccactt acaatgagtg gtgtatattg ttcaaagaat taggtctgaa
    21841 actaattgaa tgtataccat gtttagaact tgaaaatgat gttgaagagg aaatgaactg
    21901 gattataact cgtgcaaatg agctaattaa aaaatatcct tctcaaaaag atatgtttga
    21961 acaatatgtt attagccaaa aaaatgaata tactgattta cagcaggacc ttgttggagc
    22021 aacttggatt ttacaaaaag aataagataa atattagact agataatcaa tgttgtagtg
    22081 aattatccca tgtctagtga atataaataa cataaaaaac cgtcaagatg aatagtttat
    22141 tcgtgcttga cggtttttag tatttgttga agtaaaattt tatttcttta gtatatcact
    22201 tttagtcaat ccatttgatt tagcttgagt attccattga tgattcccca taagtttata
    22261 taggaatcta gggaagttag gtgaaataaa aatagtactt ttgttggaat catctatatg
    22321 tatattatca ctaagtatat tttttgaaag ttcttttaat gcaattccta aatctttttt
    22381 aggtccttta cccaaaggta cggattctag tgaaccaaac atctccccag ctccaattcc
    22441 aataccttga acccatgtaa agttgatttt tttgcaccaa tgatctatca tttgaattgc
    22501 caaatgatta tgttcacctt cattgaatcc acagttaact attgcataca ctggaatagg
    22561 ttttttattt tgtgttttta aaaatttttc acatttctct aaaaaatgaa gtaaatgaga
    22621 tggaatacca tctacataaa gaggaaatgc tattattaat ctatcacaat taagtatggc
    22681 attaagagcg tcaggatact tagatgtgct atctacttta aatgtaaaaa ctttattatc
    22741 attttgaata agttgtttta attcagataa aaaatactgt gaattatttt tatttacttt
    22801 tggactacca tttatcatta ctatattcat atggattcac ctcttatatc ctgtatattt
    22861 tcatagaaac aaacagagtg ttcttttata ttgaaattta tagcatttcc atttactaat
    22921 ttcgtagcag tttctttttc agcttctgta atacaatctc catagaatat agcttttaaa
    22981 tttatctgtt tatcatatct gcttttatga cgcatcatat tatcttttat tacaaagtat
    23041 ggaagaatat aattaatgct tttatcaagt acgtttttga caaaagggct aaaccctccg
    23101 taacagcatc tgcttattat aataattcca tcggattcat ttataagttt acccatgttg
    23161 ctgtaataat cttttacaac acattcagaa ggagttttaa tccagcaacc aaaacgacca
    23221 atacatttgt ggatttcttt tgtgtttgga agtattttta tattgtcatc aagcttgggt
    23281 aataactttt ctaattcttt gtcagttaaa tcatgtatta aaagcttcat aaagtccatc
    23341 tccttttatt atgtatgttt atttctaaaa attatatctt atagattttt cagtaaatct
    23401 ttacaccaat taataacaaa attataatag tcaactccat aaatcattgt ggcatacata
    23461 tatgtatatt ggtcaataca atgattttct tctacatcaa ttttttgttt acttaattta
    23521 tttagaagta attctacttc agttattaac atttttaagt ttgtttcaat tatttctttg
    23581 ggtagatact tgtaaaagaa tatattaaca aggtggtctt gctttgtttt agaaaaattt
    23641 ataggtttct ttaaccattc gagaaatact tctttaccag tattagttat ggaatatagt
    23701 ttttttaatt tgctaccatc tataacttcg tgatagtgta tatatccttt tgtttctaat
    23761 cttttaagtg caggatagat acttccaaag ctggcgtcaa aaaagtacga ggtactttct
    23821 gacattattt gttttaagtc gtatccactt aattcttttt ccattaaaaa tcctaaaata
    23881 atgtattcta acatattacc tcctggtatc aatcagatat atatcgattc gatatattta
    23941 ttattacaga taataaatat tacgtcaaga tgctttttaa aataagctaa aaagaattag
    24001 gaattattga taaatatgca aaaaaattaa tataaaaggt aattaaatat caatgaagat
    24061 ttaaaaatag ttaaattaaa gtaagagtct aatatataaa atttatagta actttatggt
    24121 ttaaatataa acttgtgtgc tattgacaaa gtataaaatt agcagtaaaa taattgcatg
    24181 agcaaataga ttattgcgaa cgcaaaaaaa tgaggacaat agatattaaa ggagagaata
    24241 tgagagagct attaaaatgg gtttccatta taaatagata caatacaaat tatattgaca
    24301 ggaaattggc ggatattgga atcaataata gccaatattt ttatgtatta cacatatgta
    24361 ataatccagg tattacacag gacactatat ttgagaaaat tcttgtgaat cctagtaata
    24421 taacgagagc tttggcaaat ttagaaaaag aaggctttat aacaagagaa ccaggtgtaa
    24481 aggataaaag aacatggcat ttatatccta cagaaaaatc tttagaatgt tatgatgaaa
    24541 tactaaaaat aacaaatggg tatgttgagg agttgttgag ttcatttgag aaagaagagc
    24601 aagagttgtt tatgtcaatg ttaaagaaag ttgcattttt agctattgat atgaatgaag
    24661 aagcaaaatt ggaggcgaag gaaaatggaa gaaaaatata aggagccatt ggaatatgaa
    24721 aaggtttcaa agttattaac taagtacgca attcctagta ttatagcaat gttagtaagt
    24781 gctttatata acattgttga ccaaatattt attgggcaag gtgttggtgt attgggtaat
    24841 gctgctacaa atgtcacttt tccactaaca acaatatgta ctgcaattgc attattatta
    24901 ggaattggag gagcgtctaa gtgttcattg gaattaggtg ctaaaaattc agaaaaagct
    24961 acaaaagcag caggaaattc tatatgcttg atggctgttt ttggaataag tttatttata
    25021 gtagtatcaa tatttttaac accactatta aaattctttg gctctacatc agaaatacta
    25081 ccttatgcac acacatatac aaaaattaca tcaattggat taccatttct tataatgtcc
    25141 actggaatga gtaaattgat acttgcagat gggagaccaa aagcttctat gttttgtatg
    25201 ttaataggag cagttattaa tacaattttg aatccattat tcatttttgt ttttaatatg
    25261 ggaattgctg ggtctgcttt agcaactgtt attgggcaac ttatatcttt tggaataagt
    25321 gttcattata taaagaattt tcaacatatt aaattacata agaaaagttt taaattagat
    25381 agtataattt ataaacatat atttgcgcta ggttcaagct catgttttaa tcaattagca
    25441 atgactgtaa cacagatagt tatgaataat acattggcat attatggggc taggtcagtt
    25501 tatggaagtg agattccact tgcttgtgtc ggtatcatta ttaaagtaaa tatgattttt
    25561 atatctataa ttattggtat atcacaaggt atgcaaccaa ttatagggta taattatggg
    25621 gctgaaaagt ataatcgtgt aaaagaagca tataagtttg cagtgggaag tgcaactatt
    25681 atatctgtta ttgcattctt atgtttccaa ttcttcccaa gacaaattac gtctatattt
    25741 ggtaacggaa atgagactta ttttatattt gcagaacatt attttagaac tttcttgttc
    25801 ttaacattta taaatggtgt tcaaatagta tcatcaactt tctttacatc aattgggaaa
    25861 tcagtgttgg gactttttac ttctttgact cgtcaaattt tatttttaat tccattgata
    25921 gtaattttac caatgtttat gggaattgat ggagtaatgt ttgcaggacc aatagcagat
    25981 gcagcagcgg caatggtatg tatatacttt gcagttcatg aaatgaaatc cttgactata
    26041 aagcaaaata atttgatgag taaccataat aagattgcag aggtataaga caatatttaa
    26101 gaagtataga aaattaattt aagaaatata ataaactaat ttaggaagtg
//
LOCUS       DAELSY010000045        25419 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.045, whole genome shotgun sequence.
ACCESSION   DAELSY010000045 DAELSY010000000
VERSION     DAELSY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25419
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.045"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            105..1025
                     /locus_tag="KPG09_001640"
     CDS             105..1025
                     /locus_tag="KPG09_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBG0966257.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFIPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            1140..2297
                     /locus_tag="KPG09_001641"
     CDS             1140..2297
                     /locus_tag="KPG09_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG0966258.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(2382..3881)
                     /locus_tag="KPG09_001642"
     CDS             complement(2382..3881)
                     /locus_tag="KPG09_001642"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBG0966259.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
     gene            4477..5901
                     /locus_tag="KPG09_001643"
     CDS             4477..5901
                     /locus_tag="KPG09_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBG0966260.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDALFKRLESK"
     gene            5936..8065
                     /locus_tag="KPG09_001644"
     CDS             5936..8065
                     /locus_tag="KPG09_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG0966261.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKAFIDK"
     gene            8095..8517
                     /locus_tag="KPG09_001645"
     CDS             8095..8517
                     /locus_tag="KPG09_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0966262.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            8536..9687
                     /locus_tag="KPG09_001646"
     CDS             8536..9687
                     /locus_tag="KPG09_001646"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG0966263.1"
                     /translation="MKKAIQFGAGNIGRGFIGSLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEEILNNIEKSYIFMKNL"
     regulatory      9793..9894
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            10077..10649
                     /locus_tag="KPG09_001647"
     CDS             10077..10649
                     /locus_tag="KPG09_001647"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBG0966264.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            10713..11363
                     /gene="fsa"
                     /locus_tag="KPG09_001648"
     CDS             10713..11363
                     /gene="fsa"
                     /locus_tag="KPG09_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG0966265.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGAFLKL"
     gene            11515..14439
                     /locus_tag="KPG09_001649"
     CDS             11515..14439
                     /locus_tag="KPG09_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0966266.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYGTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
     gene            15028..15486
                     /locus_tag="KPG09_001650"
     CDS             15028..15486
                     /locus_tag="KPG09_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0966267.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFILGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLRTLKSL"
     gene            15547..15822
                     /locus_tag="KPG09_001651"
     CDS             15547..15822
                     /locus_tag="KPG09_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0966268.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            15881..17155
                     /locus_tag="KPG09_001652"
     CDS             15881..17155
                     /locus_tag="KPG09_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG0966269.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            17285..18337
                     /locus_tag="KPG09_001653"
     CDS             17285..18337
                     /locus_tag="KPG09_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG0966270.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     AVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDIDPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            18337..19368
                     /locus_tag="KPG09_001654"
     CDS             18337..19368
                     /locus_tag="KPG09_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBG0966271.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKDIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            19460..20272
                     /locus_tag="KPG09_001655"
     CDS             19460..20272
                     /locus_tag="KPG09_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG0966272.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            20275..21195
                     /locus_tag="KPG09_001656"
     CDS             20275..21195
                     /locus_tag="KPG09_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG0966273.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            21370..21816
                     /gene="rpiB"
                     /locus_tag="KPG09_001657"
     CDS             21370..21816
                     /gene="rpiB"
                     /locus_tag="KPG09_001657"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG0966274.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            21855..22520
                     /locus_tag="KPG09_001658"
     CDS             21855..22520
                     /locus_tag="KPG09_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG0966275.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            22630..24132
                     /locus_tag="KPG09_001659"
     CDS             22630..24132
                     /locus_tag="KPG09_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBG0966276.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTNIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNNSNLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(24444..>25419)
                     /locus_tag="KPG09_001660"
     CDS             complement(24444..>25419)
                     /locus_tag="KPG09_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0966277.1"
                     /translation="QAIIQYLVLIFFMYIYYSEAIAIVILFTLVNTVFHFYIYNFLSV
                     KFNYINNISKNIKNIENGNLKYKLEVIGDDEISTLAKSINNITGGLETAVDANIKNER
                     MKAELITNVSHDLKTPLTSILSYVDMLKNNDFDRETINDYINILDKKSQRLKLLIDDI
                     FEASKLSSGDVEFNITKTDIRELLIQSIVEFEDKIQNSSLDFIIETPEYAVFTMIDGK
                     KTWRVFENLICNILKYSMPNTRVYIDMFIKEENIILTFKNISNDKLNLKPEELIERFR
                     RGDISRKTDGSGLGLSIAQNIIELENAHMEIIIDADLFKVMLTFRRVN"
BASE COUNT        10262 a         2810 c         4553 g         7794 t
ORIGIN      
        1 acaatttgta gaatttcaaa ttccttgaca gtaataagta agtaattgta taatcaaact
       61 gcaatatatt aataatatta gtgaaaatga aaggattata aaatatgtat aaatcagaaa
      121 atttttcaaa aaaatctgta ttaataaatt catttttaag taaattttta aaaataattg
      181 gatggggttt ggtaagctta tttacattat cagccttagt actttcaact atttccatta
      241 taattggatg ggctgtaata cttcctattc aagtatgtgc agtattttta cttataggag
      301 gagtttgtat ttttaaaggt aagagattag gagaccaaat ttctcgttat aaaaaatatt
      361 gtacaattat aaacaataga aatattattc cagtagaact tattgcagaa aacacatcta
      421 aatcattaaa ttttataata aaagatgtac aaaagatgat agacaaaaac tactttataa
      481 atacttatat tgataaaaga aataatcaga ttgtgttaac aaatgaagat tttattccac
      541 caagatatga agatgtagca tatgaagtca aagaagaaga aaagactgat gaatccaatg
      601 aagttgatac aattataaat aaaggaatga attatctaac acaaataaaa gaagctaata
      661 aaaatattaa atcagaatca atgggcgata aaattgtaca agtagaagat gttacaagta
      721 aaatatttga tgttgtaaaa catgacccat caaaacttac tcaaatacaa aaatttatgg
      781 attattatct tccaaccact ttaaaactat taaattcata tcatacattg gaagaacaag
      841 gaattgatag agaaaatata acaacaacta tggaaagtat tgaaaataca atgaatacga
      901 ttgtagtagc ttttgaaaat caattggact atctgtttga agatgaagcc attgatattt
      961 ctacagatat tactgtttta gagaatatgt tagtccaaga aggattaacg agtggtaaat
     1021 tttaacttca atagaaaaaa tattctgtta tttttaaaca attaattagt cataaagtat
     1081 aaaatgtgta ttatataagt taatcagtac tttatgaagg aagaggagag aaagatacaa
     1141 tgaataattt agatgatata ccagttatgc caactttgac acttgaccca tttggagaag
     1201 aatcaaacac taatgatata gataattctg acttattaat gaaaaaggat gaaaaagacc
     1261 cagaagaaga aaaactgtct gaatctgaac gcaagatggt aaaggaattt gcggaaaaaa
     1321 tagatattac aaatacaaat atgattttgc aatatggagc tggtgctcaa aaaaaggtag
     1381 caagtttttc tgaaacagct ttaaagagtg taaaaacaag agatttagga gaagttggag
     1441 atatgcttac aaatctagtt actgatttaa aagcattttc agcagatgaa aaagaacaaa
     1501 gtggtttttt aggaattttt aaaaaggcaa ataataaaat ttcaaattta aaaactaagt
     1561 atgatagtgc agaggtaaat gtggataaag taagtaaaga gcttcaaaag catcaggtta
     1621 aaatattgaa agatatagca atgcttgata agatgtatga attgaactta gcttactcaa
     1681 aagagcttac tatgtatatt atagcaggaa aacaaaaatt aaaggatatg aaagaatatg
     1741 aaatgccaaa gttgagagaa aaagctcgtt tatctggttc aacagaagat gctcaatctg
     1801 taaatgatat ggtttcactt tgtgacagat ttgaaaagaa aattcatgac ttagaattaa
     1861 ctagaatggt atcgcttcaa atggctcctc agataagatt ggttcaaaat agtaataatc
     1921 tgatggcaga aaagattcaa tcaactatag taaataccat tccattatgg aaaaatcaaa
     1981 tagtgcttgc tcttggaatt tctcacttaa atcaagctat gaaagcacag cgtgaagtta
     2041 gtaatatgac aaatgagctt ttattgaaga atgcagaaac tttgaagatg ggaacaatag
     2101 aaacagcaaa agaatcagaa agaggaattg tagatataga cacaattaag aaaactaatc
     2161 aatctctaat atctactatt gatgaggtag ttaaaataca acatgatggt agaataaaga
     2221 gacaagaagc agaagttgaa ttatcaaaaa tagaaaatga acttaaaagt aagcttcttg
     2281 aatttacagt taaataaaaa ttatactaat ataaaaagag actgtctcaa aatagagata
     2341 agtctctttt tcattgttta aatgattaaa gtatatttat attataaatt tctattatct
     2401 tttaacataa catcatgagc tccaccttca actatactag tagaagatac aagtgtaagt
     2461 ttagcatttt tttgaagttc aggaatgcta agtgctccac agttacacat agtagaacga
     2521 actttactta aagaaagagt tacattgtct ttcaaagaac cagcataagg cacataagag
     2581 tcaacacctt cttcaaaaga aagtttctta tctccaccaa ggtcatatct ttgccagttt
     2641 ctagcacgag cagaaccttc tccccaatac tctttcatat aactaccatt tatattaact
     2701 ttgttagttg gactctcatc aaaacgagag aagtatctac caagcattat aaaatctgca
     2761 cccatagcta aagctaaagt tatatgatgg tcataaacga taccaccatc agagcaaatt
     2821 ggaatataaa tacctgtttt ttcaaagtat tcgtctctag cttgagcaac ttcaattata
     2881 gaagtagctt gtccacgtcc tatacctttt tgttcacgag ttatacatat agaaccacca
     2941 ccaataccaa ctttaacgaa gtcagcacca gcttcagcta ggtaaaggaa tccctcgcgg
     3001 tcaactacat ttccagctcc taccttaact gtatcaccat atttttcacg aatgaaatca
     3061 agtgttattt tttgccactc agaaaaacct tctgaagaat ctatacataa aacatcagca
     3121 ccagcttcaa ctaaagcagg aacacgttca gcaaagtctc tagtattaat accagctcca
     3181 actacatatc tcttagaaga atctaaaagt tcaagtggat tttctttatg agaagagtaa
     3241 tctttacgga ataccatata cataagatta ccattatcat caagtacagg aagagaatta
     3301 agtttgtggt cccatataat gttgtttgct tcctttaaag taacatcttt gttagcataa
     3361 actatagatg acataggagt cataaattca ctaactttag tatctaaatc catacgactt
     3421 atacgataat cacgacttgc aacaataccc aataatttac cagttgaagt tccatcttca
     3481 gtaacagcta ctgtagaatg tccagttttt tcttttaagt caagtatgtc ttttaatgtg
     3541 ttatcaggtt taatatttga atcacttaca acaaaaccag ctttgtggga tttaacacgt
     3601 gctaccatag cagcttcatt ttcaatagtt tgagaaccgt atataaatga aataccacct
     3661 tctttagcaa gtgcaatagc cattttgtca tcagaaacag attgcataat agcagaagtc
     3721 aaaggtatat tcatataaat atctgcttcc tcgccctttt taaatttagt aacaggtgtt
     3781 tttaaactta cattagcagg tatacaatca gtagaagaga aacctggtac aagaaggtat
     3841 tcactgaaag tatgtgatgg tgtatcaaaa taaaacgcca ttttttaatc actccttata
     3901 tttttatact ttttataatt gatagtatta attataactt attctttaat tttattaaag
     3961 tattttgtca aatattgaga tttttgaaaa atttaaacta ttttaattaa atttgtttaa
     4021 tatatattgc cttatagggt aaagttgtat attaaataat gtttagtgtt taatagtagc
     4081 taatttaaaa ttaatcttta gtgattcata taattacatt tgtatagttt atattgataa
     4141 tattactaga gagtgcataa acctcaaaaa tacagataaa ttaaatctat attagtatat
     4201 ccaaaagtac atttaataaa tttatttaac tgaattatta ttttcatttc tattaaattt
     4261 aaatatttta taaaaatagt attatttaca aaatcatata ctccaaaata ctacataaat
     4321 aaaattaaac aaaaactaaa atgacaaatg tcattatcta aagtgacata gaaacggttt
     4381 cttaaagttg ttactataaa atataaactc tataataaaa gtataacaaa agggaatcat
     4441 atcgatatag aatataattc aagaggaggt aaaattatga gtagttctaa tcaatcgaca
     4501 ataaagacat ctatacagcg agtagggaaa tttttaagtg gtatggtaat gccaaatata
     4561 ggagctttta tagcatgggg tcttataaca gcattattta taccaacagg atggatgcct
     4621 aatgaaaaat tgagtactat aggcgaccca atgataaaat accttttacc actattgata
     4681 gcttatactg gtggtaaagc aatagcagga caaagaggtg gcgtaatagg tgcagcagca
     4741 gctatgggag taattgttgg tgcagatata ccaatgttta tcggagcaat gataatggga
     4801 ccatttgcag gatgggtaat taagaagttt gataaatttg tagatggaaa gataccaaca
     4861 ggatttgaaa tgttagtaaa taacttttca ataggtataa taggtatgct tcttgcaata
     4921 ctaggatttt atgcaattgg accagcaata gtagcaggga cagctcttat agaatctgga
     4981 gttcaattta tagttagcaa aagtttattg cctctagttt cagtatttat agaacctgga
     5041 aaagtattat tcttaaacaa tgcaataaat catggtatat tagggcctat aggtatagct
     5101 gaggcaaaag aagcaggtaa atctataatg ttcttactag aaagtaaccc tggaccaggt
     5161 ttaggagttc tattagcata ttggatgttt agtagaggaa gtgtaaaaca atcagcacca
     5221 ggagctgtaa taatacattt ctttggtggt atccatgaaa tttacttccc atatatttta
     5281 atgaatccag ttttaatatt ggctacaatg gcaggtggag cagcaggaat attgactttt
     5341 tcaatattag gagctgggct tgtagcagca ccatcaccag gaagtatatt tgcacttatg
     5401 gcactagctc caaagggagg attacttcca gtattagcag gagttgcagt ggcaacagtt
     5461 gtatcattct tagtggcagc accatttgtt aagagagctt cagcaaatca aagtgaagaa
     5521 gacagtactt ctttagaaga agctaaagca aaaatgagtg atatgaaatc tgcaagtaag
     5581 aactctgaga aaaatattga ggaaaaacag cttgaagtaa atgagattaa gaagattgta
     5641 tttgcatgtg atgcaggaat gggttcgagt gcaatgggag cttccagatt taaaaataga
     5701 ataaaaaact tagacttaaa tatagaaatt acaaattcat ctgttgataa tcttccagat
     5761 gatacacaaa tagtagtaac tcacaataca ttagttgaaa gagttgctaa gaataattca
     5821 agtgttgaga tagtttcaat aaacaatttt ttaaatgacc caaatttaga tgcattattt
     5881 aaaagattag aatctaaata aaaataaaaa gtaccactaa ttaggagtgg ataatttgaa
     5941 aaaaaagaag attacatcta ggcaaaaaaa gataatacta atgatagtag aaaattcaaa
     6001 gaaaaacatt ccaataacta tttctgagat tgcagggact ttagagctaa gttcaagaac
     6061 tgtacttaga gatatgtctg gaattgaaaa atggtttgat gaaaatgatt ttaactttgt
     6121 aaaaaaacca ggagtgggat tgattttaga ggaaaatata gaaaatcaaa actttataat
     6181 tgagttgtta gaagaagaaa aaatagaaaa agaatacagc aaggaagaaa gaaatttaat
     6241 tatattatca aagctactag tttctaacga gccagtaaaa tcctattatt ttgcaaaaat
     6301 attaaaggta tctgaagggg tactgaataa tgattttgcc ttagcaagta aatggttgga
     6361 aagatttgat attgagcttg ttagaaaacc agggcttggt gtctacttaa aaggtcaaga
     6421 aaaaaacttt agagaggcat atgtaaatct tatatacgat tcattcaatg aaaaagaaat
     6481 actagatatg gtcagaaata taagtgaaaa tatacaaaca gataaagcaa tagaaatttt
     6541 aagtgaaaat agacttttaa atttaatgga caggtgcata ataagaaaag ttgaagctac
     6601 attaacaaag aagctttcag atttagatgt aaatttagct gatagtgcat atatagggct
     6661 tgtagttcat atttcattag ccttacagag gataaaaaat ggtgaaaata taactatgga
     6721 taaagagttt ctaaaagaat tatctataac agaagagttt aaattagcta aagaaatcgt
     6781 aaaaggtatg gaaacagatt ttaacatgga tataccagta gatgaggttg gatatataac
     6841 tatgcatata agaggtgcaa agcaaaggtc atcttcaaac cataaggctt taaacttaga
     6901 tgatatagaa ataatggaaa ttacaaataa gatgatagat ttggcggaag atgaatttaa
     6961 aataagttta aaaaatgacg agaggttatt taaagaccta gcaaatcact tagggccttc
     7021 tataaataga ttgaatatgg gattagaaat aagaaatcct cttttagatg aaataaaaag
     7081 caagtattca tatgcctatg atggagttga aaagatttct agaattataa aagataaact
     7141 aaatataaat tcaatacctg aatcagaaat aggatatatt gcaatgcact ttgcatcagc
     7201 catagaaaaa aatcttatga tgaatactaa tataaatatt gttgtggcat gtcccactgg
     7261 aataggtact tcaagattct tatcaactaa gattgaaaat aaatttccta atttaaatat
     7321 acttgaaacc atatcagcta taaacataga tgaagagtat ttaaaagaaa aagatgttga
     7381 tttaatagtg tctacagttg agttaaatac aagtttaaat tatatatgtg taggtccatt
     7441 tatgagttta gatgatgaac agataatcaa ggaaaaaata aaatctatag ctcaaaataa
     7501 attaatcaat ttaaatgcga aaaatgatac taaaagtaag aataaagtat atgaacagat
     7561 aacagaaagt atgaatatag gaaaagatat tcttcaattt ttagaggaaa ttagatttga
     7621 aaaatttgaa agtaaagatt taagtgagct tattgaagac tcatcaaaaa tatttgcaaa
     7681 atctagtgaa gatataatct ctataaaaga atcgttaaaa gaaagattaa aaatctcaat
     7741 tccatatata gaagaatcaa aaattctatt gttacattgt atgagtgaaa gaatagatat
     7801 tatgaagtta tcaataataa aactagagaa tagaatcgtg ctagattcaa atgaagaaat
     7861 cgataatgta gtttttatgt tacttcctaa aaattcacca agttatcaaa gacaaataat
     7921 gagtgaaata agtggttcat taatagacaa tataattttt actaataaga taaataaatt
     7981 ctctatagaa gagatgacat tagaggtaaa agatatagtt tttaattttt atacaaacag
     8041 attaaaagca tttatagata aataaaatta atattaaaaa ggaaggttga tactatgagt
     8101 aaagtattga atgagaataa tatattttta gggttagata gtgtttcaaa ggaagaagct
     8161 ataactttag caggaagaaa attagttgaa aatggatatg taaaagaaga atatatacca
     8221 gcaatgttag aaagagaaaa agtaatgact acatatatgg gaatgggagt tgctattcca
     8281 catggtgtaa atgaagcaaa aaaagaaata ttatcttctg gaatagttat attacaattc
     8341 cctaatggta tagattttga tggagaaaaa gcatatttat taataggaat agctggtgtt
     8401 ggagatgaac atttagaaat actatctaat atagcagtag ttttagatga tgacttaaca
     8461 gaaagactaa aaaattcaaa tgacaaacaa gcttttatgg aagcttttgc caactaatct
     8521 taggagggat ttaaaatgaa aaaggcgatt cagtttggag caggaaatat tggaagaggt
     8581 tttattggaa gcttgttagt taagtcgggt tatcatgtag tatttgctga tgtaaatgag
     8641 gagatattaa actcaataaa caaagataaa aagtatacta tacacataag agatgtagaa
     8701 tgtatagatg aagtaattga taatataagt gcggtttcat ctataaaaga agaaataata
     8761 gatgagattg tacaggctga aataataact acagcagttg gacctttagt tttgactaaa
     8821 atagcctcta caattgcaaa aggtataaaa gctagaaaag aaaagggatt aacaagtaat
     8881 ctaaatataa tagcatgtga aaatgcaata tacgcaagtt cttctttaaa agaagaggta
     8941 cttaagtatt taaataagga agaagtagaa tatttagaga tgtatgttgg ttttccaaac
     9001 tgttcagttg atagaatagt tcctcctggt aaaaacgaaa atcctcttga tgtaacagtt
     9061 gaaaattttt atgagtggaa tgttgaaaaa caaggattta aaggggaaat accaactata
     9121 gttggaatga atttagctga taaccttatg gcttatatag aacgtaaatt gtttacactg
     9181 aatacaggtc atgctataac agcttatata ggatatttaa aaggatataa gactatagaa
     9241 gagagtataa aagataaatt tatctgtgat atagtcaaaa gtgcaatggt agaaagtgga
     9301 gaaggattga ttaaaaaata caattttgac tcagaagtgc actataaata tattgataaa
     9361 attttaaata gatttaaaaa tccatatctt aatgatgatg tattaagagt cggaagggaa
     9421 ccattaagaa aactaagtga taaagataga ttgattaaac ctttaatgac agcaaaatca
     9481 tatggattat cagttgacaa tttgattctt ggaataggtg cagcgcttca ttataataat
     9541 agtgaagata ctcaaagtgt agaattacaa gagttgataa agtctattgg ggtaaaaaaa
     9601 gccgttgcta aaatagctaa tataagcaat gacgaagaaa ttttaaataa tatcgaaaaa
     9661 agttatatat ttatgaaaaa tttataaatt tacttgaaaa aaagtagttc tgataaagta
     9721 actataatat attattctac acagtttgaa ttatttgttg aataatagag aattataata
     9781 tataattaaa aggaataaat taatatattt acctgtatat gtttgataat atggttcaaa
     9841 tgtttctact aagctaccat taatagcttg actacaggag tgaagaagat atagtttata
     9901 cttattttaa attatatatt aagttaatta ctttgaattt taagctcctg tctatgatat
     9961 agacaggagc ttttttagta ttttaatata aaatacttac aagaatagat agcaaaatta
    10021 gaacaaatat aattttttta ttaatataat tttagaaagg gctaggtgga agttcaatga
    10081 aggctttaca agaaaagata ttgagagaag gcagtgtttc aggaaatgat atactaaaag
    10141 ttgatagttt tttaaaccat caaattgatg tagctttttt aaatgaaata ggaagagaat
    10201 ttaaagagag atttaaagga gaaaaggtgg ataagatatt tacaatagag gcttcaggaa
    10261 tagctatagc atcaattgtt tcacagtatt ttgataatgc acctgtagta tttgcaaaaa
    10321 aatcagaatc taaaaatttg gataaagatg tatatgaaac taatgtatat tcatttacta
    10381 aggcaagaga gtattcagtt aaagtttcta aaaagtacat aaacaaaggc gaaaatatat
    10441 tgatagtgga tgattttttg gcaaatggaa gagctgctct tggacttaaa gatttaattg
    10501 agcaagcaga agctaactta gttggagtag gaattgtcat tgaaaaagga tttcaagctg
    10561 gaggagcact tttaaaggct aatgatgtaa gattagagtc tttggcagta gttgaatcta
    10621 tagataatgg aactgtaaaa tttaggtaat ttatgatatt atataattgt gtttagcaca
    10681 tattttaact atgtaattat ggaggatttt ttatgaggtt ttttatagac acagccaata
    10741 ttgaagaaat aaaagaagca aatgatttag gtgtaatatg tggtgtaact actaatccgt
    10801 ctctaatagc taaagaaggt agagatttta tagaggtagt taaagaaata agtgaaatag
    10861 tagatgggcc aataagcgca gaagtaataa gtttagaaca taaaggtatg atagaggaag
    10921 cagaaaaatt atctaagata cataaaaata tagttataaa aatacctatg acagcagaag
    10981 gtctaaaagc ggtgaaagta ctatcaagta aaggtataaa gactaatgta acgcttatat
    11041 tttctgctgg acaagctctt ttagctgcta gagctggagc tacatatgta agtccttttg
    11101 ttggtagatt ggatgatata tcacaaaatg gtcttgattt aattgaagaa atcgttgata
    11161 tatttagtgt aaatagtatt gaggctcaaa taatagttgc tagtgttaga aatccaatac
    11221 atgttttaca agctgctaga atgggagcag atatagcaac agttcctctt aaagttataa
    11281 accaaatgat taagcatcct ttgacagaca aaggaataga tagctttatg aaggattggg
    11341 aaggtgcttt tttaaaatta taattataaa ctacgcttta taagtatatt aaagaggtta
    11401 ttatggaaat tctaagtaat aaggctaggc tattttgcct agtcttattt tggtgtataa
    11461 tatattgaaa catattattg aaatagatta attttttaag tgaggaggcg aaaaatggat
    11521 aaaatattaa gagagataca aagagaagat aaaaaaaatc catatactga ccaagaactt
    11581 gctgaaatgt taaatgtagc taggtctgaa gtgatatcag ttaggaaaaa aaataatatt
    11641 ttagattcga gagaaagaag aaaaaaaata ttaataaaag atatatcaaa aattgtaagt
    11701 gaaaatcctc aaatgtctga aagaaaaata actgaaatat taatagaaaa tggatataat
    11761 atatcgagaa gtgcagtttc aaaattatta aaagaggaaa atttaaatca gtcaaagatt
    11821 gggattaagg ataaagaccc agcatcatca ggaaagtatg tgaaggtgag taaagttgta
    11881 gaagaagatg gatttgaaga attgataggt ataaagggaa gtttgaaaga aaaggtaaat
    11941 ctagctaagt cagcaataat gtatcctcct aatggccttc atactataat atatggagaa
    12001 acaggagtag gtaaaagtga attagctaca tgtatgtata aatatgctat aaaaaacaac
    12061 attaaagaag agaacagccc atttatagtg tttaactgtg cagactatgc agaaaatcct
    12121 aatctattga tagctcaact ctttggggta gtaaaaggtg catatacagg agcagatgca
    12181 aatagagaag gtcttgtaga acaagctaat aatggaatat tgtttttaga tgaaattcat
    12241 agattgcctg ctgctgggca agaaatctta ttttcattaa tagatagagg agaatttaga
    12301 aggttaggag aaagtagttc taatagaaag gcaaatgttc tcataataag tgctacaaca
    12361 gaaaatccag attctaattt acttcaaaca tttagaagac gtataccaat ggtaataaat
    12421 ttacctaata tgagtgaaag accaaaatct gaaagatatg aaataataat aaagtttttt
    12481 gaaagagaag caaagcgagt aaacaagaat tttataataa ctaaagaagt tatgtgtgca
    12541 cttatgaatt ataagtgtac tggaaatata ggtcagttaa agagtgatat acaagtaact
    12601 tgtgctagag cttttagtaa atcgatattt tctagcgata aggtaattat agatttagat
    12661 agtttaaggg attatataaa aagtggatat tatgattgta attgtaaaaa tgaaaagtat
    12721 tctgagtttt cagttgagga tatatatata gatattagtg aattggtgag tggaaaaaat
    12781 agaattttat ctgataatga gataagcgag atatataatt atgcagaaaa agagcttaag
    12841 gttttggaaa gtaagtgctt ttcagaagaa gagttaaaaa atgcatttat agaaaagtta
    12901 gataagaagt ttaatgctat taaaaacaat aaaaatttat ctgatagaag aagtcgaata
    12961 gactggggag gacaattaaa agagtctaca ctggagataa tggataaggt aattgtattt
    13021 attaaggagc agtttaagca tgtaaatcaa ggtctgtact tggctctagc aattcatatt
    13081 gagcatgcta taggtaggat aaaagatgga aaaactataa taaatccgag tttagataaa
    13141 ataaaagcat caatgccaat ggaatatgag ttgtcaagat atataatggg tatagtagaa
    13201 gatttgacac aagtaacatt tccagaagat gagcttggat atttggctta ttatataaat
    13261 aaattttgtt ataatgagga aagtataaaa gataaagtta aagtagtaat agttacgcat
    13321 ggtaaagttg gaattgaaat gtctaaagtt gtaaatcata ttttaggtat agaatgtaca
    13381 ctaggtatag aaatagcact tacagattca ccatcagagg ggatagagca tgtattagaa
    13441 gagcttcaaa agatagaagc tagaaaaggt attctagttc taattgatat gggttctttg
    13501 gtaattcttg gtgatgaggt tgaaaaaagg cttggaatta gatgtaaaac tgtaaataga
    13561 gtagatacat tattggcaat ggaagcagga aaacttgcaa ccattgaagg aaaaagttta
    13621 gatggtataa tagctgattt aaagaaaaat aagaattatg ctatgataaa tacaaataaa
    13681 tttagttaca gaaaaaacga gtatggaaag aaaaatgtaa tcattacttt atgtttgagt
    13741 ggtgtaggta cagctttaaa tttaaaagag catatagaaa aacaaattaa ggaatatggc
    13801 actttaattg aagtaaagcc agttgctttt ttaaataata atttagaaga agagttaaat
    13861 gatattgaag ctgaatataa tattattgct atctgtggaa ctattgatat agattataaa
    13921 aacgtgcctt ttatttctta taatgaggta cttggtaaaa atggtattaa taaagtatta
    13981 gagcatctaa atagtaaaca accagcttta aatattgaaa accagttgaa taatttaata
    14041 catgaagatt tgattttata caattttgaa ggaatttcaa aggagtatat aattgatact
    14101 cttgtttcaa aacttgaaga aggtggatat gttgattcaa agtatatatt aagtgtatat
    14161 aaaagagaag caatgggaag tgttgtaatg gcctctaagg tagccgtgcc acatggactt
    14221 ccagaaaacg ttataaagcc tgcaatagca atcgcaaggt tgaataaacc tatagtatgg
    14281 gacaataaat ttatggtgga tttggtggta ctactggcac ttaaagaaaa taataagaaa
    14341 gaaatacgaa gcttattttc aaagattaat gaccagcata ctttagaagt tttattaaat
    14401 actgaagata aaaatgaaat aaaagaactt ttaagctaat tattagtacc gagaagaaag
    14461 acaaaaaaat ttgctttagt ataaaattta aaattactca attgctaagt agttaattac
    14521 tctatataag gctagttcta tgaaatttat ttatatgatt ttatagaatt agcttttttc
    14581 ttggtaaata aaatatatga atttatttat gtaaaaagta aattttattg gacaattcta
    14641 tgaaaatttt tcctatattt cttataaaat atagaatata taaaaaatat ataaaaataa
    14701 attaaattca taataaataa ttttataaaa tagttgttaa acataaaaaa ccatttgtaa
    14761 aaaacaaaaa aatcatttct ctaacaaaaa aaaccacaaa aaatataaaa aagtatggct
    14821 agttaagtga ttaacaagta aaaattaaaa taaaaatcaa aaaaaattta tcaatgaaag
    14881 tcagctaaaa tcctggaaga gagcattatt ttcgggaaaa cattttcttt tgtttcacaa
    14941 aaaaagccac tttttggcac gcaacatgct ttatataaag tcaagaaaaa ctaaggtata
    15001 agacaaaggc aattaggagg tgtaaatatg gagtttataa attttgtaag tgataaagtt
    15061 atagacctta aaatggattg taaaacgaaa gaagatttct ttaaagaaat acataataaa
    15121 gtttttgatt taggctttgt aaaagaagaa tttggagaaa aaatattagc aagagaaaat
    15181 gtatttccaa caggtttaaa tttaggtgat tatggagtag caataccaca tacagatgca
    15241 gaatatatta aagagcaatt tatatcagtc tgtactttta atgaaccagt agtatttagt
    15301 tctatggaag accaagatga aaaagtacct gtaaatttgg ctttcatatt aggacttaat
    15361 caaccacaca gtcaattatc tgtattaaca gaattaatgg gaattatgca aaataaagag
    15421 ttagttgata agttgatgag ttctactgat aaagaagaag ttttaaggac actaaagtct
    15481 ttataaaaat aaaaaagtac taaaaataaa aaatataaaa ataaaaatta ttaggaggaa
    15541 ataaaaatga aaaaaatatt agtagcatgt ggagcaggaa tagcaacatc aacagtagtt
    15601 tgtgacaagg tagagagatt agttaaggaa aataatatac aagcagaagt tatacaatgc
    15661 aaaatagctg aatgtgcaac aaagcaagaa ggagcagatt taatagtatc aacaactata
    15721 ctaccaacaa cttataatat accagcaata aaagcaacag catatataac tggagtaaat
    15781 acagcagcat tagataaaaa aatattagat gctttaaaat aatcctatca tagatacatt
    15841 gaaataatat tttataaaac agaaaaagga ggatttataa atggataaat tattagcagt
    15901 agtacaatac attttaaata tgggaccgac agtaatacta ccaataacaa ttgtagtact
    15961 aggaatgata tttagaatta agtttacaaa agctttaaag tcaggtctta caattggtat
    16021 aggatttgtg ggtataaact tagttgtaaa cctattaaca tcaacactag ggccagcggc
    16081 tcaaggaatg gttgaaagat tcggacttaa tttaacagta atagatgttg gttggccaac
    16141 aacagcatca gcaacatggg cttcaccagt tgcaccaata ttaataccag tttgtttatt
    16201 agtaaactta ctattattat acttcaataa aacaaagata ttaaacgttg atatatggaa
    16261 ttattggcac tttatagtag caggtgcagt tggatatata gtaacaggaa gttatgtttg
    16321 ggctatattc tgtgcaatac taatggagtt attagtactg tttattgcag aaaagacagc
    16381 aaaaaatgtt caggagtttt atgatttaga aggtatagca ttaccaactg gttcaacagc
    16441 ttcatttgct ccacttggat atttagtagg taaatttgtt gaaaaaatac caggaataaa
    16501 taaaatacaa gcagacccag actctataca aaaaagattt ggtatatttg gagaaccaat
    16561 gatgatgggg attatactag gtataatact tggagcactt gcgggatatg atgtagcagg
    16621 aatattcaat gtaggtatat caatgggtgc agtaatgtta cttatgccta gaatggttag
    16681 aatattaatg gaaggtttaa ttccaatttc agaatcaata agagatttct tacaagcaaa
    16741 atatggagac agagaacttt atataggtct ggatgcagca gtagcaacgg gacatccagc
    16801 agttatatca acagctctta tacttgttcc tataacgttg ttcttagcag ttatactacc
    16861 aggaaacaaa gttttaccat ttggagactt agcgacaata ccattttata tggcatttat
    16921 agtgtgcttt agaaaaggta atatagttca atcagttata acaggaacaa tagtaatagc
    16981 tatatcactt tatatggcta caaactttgc agatgttcat acacaattat tacaacaagc
    17041 aaatatgatg ccagaaggaa caacaatggt tacaagtata gatacaggtg gaaacttgct
    17101 aaaatgggga atattgaaat tatctcaatt agtttcttca tttggaatat tctaatatgt
    17161 agttaataga gattactctt ttttagtaaa gtatgcgaaa atttaacatt tagcagctac
    17221 taaaaaagag taatattttt tataaatttt taaaaataca aaatacaaga ggaggagatt
    17281 tatcgtgaaa gcagcagtat tacatggcac aaatgatatg agatttgaag atatagaaat
    17341 aaaaccatgt gaaagtgatg aagtaaaaat aaaagttatg gcagcaggaa tatgtgggtc
    17401 agatcctcca agagtattaa aacattggaa atatccagtt ccagctatac caggacatga
    17461 gttttctgga gtaattgctg aagttggtaa ggatgtaaaa aatgtaaaag ttggagatag
    17521 agtagttgca attcctttta taccttgtaa tgaatgtgaa tattgtaaaa gaggattatt
    17581 ttcattgtgt gatgaccatg gaatgttagg agctaaaagt tttggagctt ttgcagagta
    17641 tgtaaatata aaagctacaa atgtacttcc aataggagat atggactttg aagatgctgc
    17701 aatgatagag ccactagcag ttgctatgca tggagtactt aatataggtg ttcaagttgg
    17761 agacgctgta gccgtaatgg gtagtggaac aatggggcag ttggtaatac aaggacttaa
    17821 aatagcagga gcaggaacaa ttatagcagt tgatatatct gataataaat taagagaatc
    17881 aaaggaatta ggggctgata taataatcaa tgctaaagat attgacccag tagaaaaaat
    17941 aaaagaactt actggtggaa aaggcgttga catagctctt gaatgtgcag gttcaaaaat
    18001 aactcaagaa caatgtttac ttataactaa aaagaaaagt aagataggat ttttagggat
    18061 agcatattca gatataactt tatcagaaga agcatttgaa aatatattta gaaaagaact
    18121 agaattaaaa ggtttttgga actcttattc tgcaccattt ccaggacaag aatggactaa
    18181 gggaatcaat ctagttaatg aaggaaagat aaaattaaaa gaaatggtat ctcatagatt
    18241 ctcacttgaa gatacttaca aagcatttga aatgataaga gatagaaaag aagaatttaa
    18301 caaaatatta attttaccac aaggggttga aaaataatga aagcagtagt taaaactaaa
    18361 ccaggctatg ataatgtaga agttttagaa gtagaagaac caaaagctac aggagacaag
    18421 gttaaaataa aagtagaata tagtggtata tgtggttcag atatacattc atttaaaggt
    18481 gaatatgcaa atataaaagc cccagtaaca ttaggtcatg aattttctgg aattgttgta
    18541 gaagtaggag aagatgttaa agatataaaa gtaggagata gagttacatc agaaactact
    18601 tttgcaactt gtgagaaatg tatatattgt gaaactaaag attataactt atgcccaact
    18661 agaaaaggta ttggtactca agtaaatgga agttttgctg agtatgtact atcaagagaa
    18721 gaaagcatac atgtacttcc tgaaaatgta tctttattat cagcagccct tacagagcca
    18781 ctagcttgtt gtgttcatgc agctcttgaa aagactacta tagaaagttc tgatacagtt
    18841 ttaataatag gaccagggcc aattggttta ttattagctc aagttgttaa atctcaaggt
    18901 gctacagtta taatgtctgg tgttacaaaa gataaggaaa gattagaaat agctaaaaat
    18961 cttggagtag atagaactgt aaatgtaatg gaagaaagtt tggcagatgt agtgaatgaa
    19021 atgactgatg gaaatggagt aaacaaagga tttgattgtt ctgggtttat gccagcagtt
    19081 aacgaagcct taagactgct tagaaaaaaa ggtactttcg tacaagttgg aatctttgct
    19141 aaaaaattaa atgaaatgga ccaagaagca ataatccaaa gagagttaca atatatagga
    19201 agtagatctc aaaagccaag ttcttggatt acagcattag atttaatggc aagtgggaaa
    19261 gtaaatacag aagctttagt tactagaact gttggtttag acgaatttaa agaaggtatt
    19321 acagctttaa tgaacgggga agaaataaaa gtagcagtaa aatcttaaaa aaacatttgt
    19381 attattttaa aaaaaattgt aaaatctact gtaggataaa aaaatgtaag gtcttaaaaa
    19441 ataacaggag gaagaaagta tgagcactct tagagatcat tctaataata ttagaaaaaa
    19501 cataataaaa atggttgcag aagctaaaag cggacatcca ggtggttcat tatcaatagc
    19561 tgatatgttg acagtattat attttgataa gatgaacgta tgtgcagaaa atccaaaaat
    19621 ggctgataga gatagatttg ttctatcaaa aggtcatgca gcaccagctt tatatgctac
    19681 attagcagaa aaaggatttt tcccagaaga agaattaata acactaagaa aatttggttc
    19741 taagttacaa ggtcacccag acatgaaaaa agtagcagga atcgatatgt ctactggttc
    19801 tcttggtcaa gggctatctg cagcaaatgg tatggcactt gcaggaaaat tagataacaa
    19861 agactataca gtatatacta tattaggtga tggagaaatc caagaaggtc aaatatggga
    19921 agctgcaatg acagcatcac attacaaatt agataattta attgcatttg ttgacttaaa
    19981 tggattacaa atagatggtt ctaatgaaga agtaatgaat gtaaatccag tagatgacaa
    20041 attcaaatct tttggatgga atgtaatagt aataaatgga cattcttttg aagaaatagg
    20101 aaatgcagtt gatgaagcta aaaaagttac aggaaaacca actgtaataa tagctaaaac
    20161 agtaaaaggt aaaggtgttt catttatgga aaataatgct gcatggcatg gaacagctcc
    20221 aaatgcagaa caagcagagc aagcattgaa agatttagaa ggaggtctat aggaatggga
    20281 atagcaacta gagaagctta cggacaagtg ttaaaagaac ttgcagaaaa taaagatata
    20341 gtagtattag atgcagattt aggaaaagct acaaaaagta tatcatttaa agaagttgcc
    20401 ccagatagat tttttgacat ggggatagca gaaggtgaca tgattggtac agcagcaggt
    20461 cttgctactt gtggaaaaat accttttgcg agtacttttg caatatttgc agcaggaaga
    20521 ggctatgagc aaataagaaa ctctgtagca tatccaaatt taaatgtaaa aatagcagct
    20581 actcatgcag gtgtaacagt aggtgaagat ggtggtagcc atcaagctat agaggatata
    20641 tctctaatga gaggtatacc aaatatggtt gtattaaatc cagcagatgc tttagaagca
    20701 agacaagcta ttcttgcatc tattgattat aatggacctg tttatataag attaggtaga
    20761 gctgcaactc ctgatgtaaa cagtgaaaat tataaatttg aaataggaaa aggaactgtt
    20821 ttaagagaag gttctgacat aacagtaata gctacaggaa taatggtagc aaaagcctta
    20881 gaagcagcag aagaacttgc taaagaaggt ataaatgtag aagttgttaa tatatctaca
    20941 ataaaaccat tagatgaagc attaataaaa gaaactgcta agaaaactgg aaaagtagtt
    21001 acagcagagg agcacagtat aataggtggt ttaggttcag ctgtatgtga agctttagca
    21061 gaaactaaag atgtagtagt tagaagaatt ggtgttaaag atgtatttgg tcaatcaggt
    21121 acaccagctg atttattaaa acactatggt ttaactacag aagatatagt aaaaaatata
    21181 aaagagttgt tatagatata aatatatcta aataacaaat tgatttgaca taagtcaatt
    21241 aaagctgtct taaaaataga aagaaatttc tacaatggga cagctttatt tttacaatac
    21301 tttattttta tgataattat aaaatagtct gaaagtaatc aaaaaaatta taaaaagagg
    21361 tgctatttaa tgaaaatatc tattggatgt gaccatggtg gttatgaatt aaaggaatat
    21421 ataaaggaaa atttaataaa taaaggtata gaagttgaag atgtgggtac aaattcaaaa
    21481 gaaagtgtag attttccaat atatgcaaaa aaggtaagtc aaaatgttca aagtaaaaaa
    21541 tctgatttag gcatactttg ttgtggaaca ggaataggca tgtctattgc agcaaataag
    21601 tttaaaggaa tcagagcagc tgttgtttct gactgttttt cagctaaggc aactagagaa
    21661 cacaataata caaatgtact ttgtttagga gaaagagtta ttgggaaagg attagcaatg
    21721 agaattgttg aagaatggtt aaattcaagt tataatggag aaagacatgc aagaagatta
    21781 caaatgattg atgatataga aaaagaaaat ttataaatat atataatata taatttataa
    21841 caagggggag cgttatgtct attatttgtg cttcaataat gtgtgcagac caaatgaagt
    21901 taaaagagga attagaagct cttgaagaag caggtgtaaa attacttcat tgtgatgtta
    21961 tggatggaat atttgttaaa aacttggcta tgggaccaga gttattaaaa tcaataagtg
    22021 aaaatacaac aataccttta gacatacact tagccacaga aactccagat aaatatattg
    22081 atatgatgtc ttatataaag ccaaaatata tttcttttca tgtagaatca agtacaaatg
    22141 taaaagctga tatacaaaag ttaagaaatt atggtatagg gccagtgtta gcaataagcc
    22201 ctcaaacaag tgtggataaa atagaagaat atataagttt agttgagggt atcttagtaa
    22261 tgacagtaaa tccagggttt gcaggtcaaa agtttaatct ttctgtattg gataagttgg
    22321 ataaacttac agagatacta aaagattatg ataatcctcc atttatagag gttgatggaa
    22381 atataaataa ggatactatt aaacttatga atggtaaaaa agtagatata tatgttgttg
    22441 gtacttcagc tttatttaat gataagcctc caatttcata taaagataaa atagaagaac
    22501 taaaagaaag tataaagtaa ccatgtaata aaattataat atgtttctgt ttaatagtaa
    22561 aaatttaggt ggaaacaaga aattttataa tatttaataa aaattttggt tgagtaagga
    22621 gaatttttta tgggagacct atataaacta caaaatggta ctgatattag aggaatagcg
    22681 tatgaaaatg attctaaaga agttaaccta actgtagaag aagtaaaaaa aatagctaaa
    22741 gcgtttcata tttggctaaa agaaaagact aagaaagata aagtaactgt tgccatagga
    22801 actgattcta ggataacagg aagccaattt agaagtactg ttattgaaac tttaactaat
    22861 gatgattgta atgtaattga ttgtgagatt gctacaactc cagctatgtt tatgacaact
    22921 attatggatg gatacaattc tgatggggcg attatgatta cagcaagtca cttaccttat
    22981 tactataatg gtttaaagtt tttcacagaa agtggaggtt tagaaaaaac taatataaaa
    23041 gagatgttgg atatagctgt taaaaatgat agtgtagact atgaggaagt aaataaaaaa
    23101 ggtgaagtta tagtaaaaaa tttaatagaa gattattcta atttattgat agataaaatt
    23161 agaaaaggtg taaattcaag taaaaattat gaaaagcctt tttctgggct taagatattg
    23221 gttgatgcag gaaatggagc tggtggattc tttgctgaaa aagttttaca catactggga
    23281 gcagatacga caggcagtca gtttttgaac ccagatggta tgttcccaaa tcatatacct
    23341 aatcccgaaa acaaagaagc aatggaaagc atatgtaaag ctgtgttgga taataatagc
    23401 aacctaggaa taatctttga tacagatgta gacagagctg ctattgtagg taaaaatggc
    23461 aaaccaataa ataaaaatgc tcttatagct gttatatcat caatagtatt agaagaacat
    23521 ccaaaaacag ctatagtaac agattcgatt acttctgaag gacttgctaa atttataaat
    23581 gaattacaag gaagacatca tagatttaaa agaggatata aaaatgtaat aaatgaagct
    23641 ataagattga atagtgaagg tgaagagtgc catttagcta tagaaacgtc tggacatgct
    23701 gctttaaaag aaaattattt cttagatgat ggggcatatt taatagctaa aattttaata
    23761 aaagtagcaa agctatcctt ggaaggaaag actatagaag aattgataga aaaccttgaa
    23821 gaagctcaag aagagaaaga agttagaata ggcattaaca aagaagattt tagaccatat
    23881 gcagaggata tattaaaaga tttagattct catgttaaga gtattgatgg atggtctgta
    23941 gcaccaaata attttgaagg aataagagtt aattgtgatg aatcaaatgg agatggatgg
    24001 tttttactta gaatatcact acatgaacca ttactagctt taaatattga atcaaataaa
    24061 attggtggag cagaacaaat ttattctaag ttaaaacttt tcttagaaaa atatgattta
    24121 aaaggtcttt agggtaaata gaaagaatat atttatatta aagaacaaat gtaaatttgt
    24181 acctaagatt acaaatatta caaaattgta atcttaggta acagagatgt aactgattat
    24241 ggaaacacta tgtgatatta tagatacaga gagatgaaat atgtgatgaa ttatagagaa
    24301 atataattta aaatgtatta atatcacacc aaaaaggtat aatcaaaaaa tatgtaaata
    24361 taaaccccaa catttagaaa aagacctcta aggaggtttt tttcttgttt aaataactta
    24421 aaatatattt ttatattaat atatcaatta actcttctaa aagtaagcat taccttaaat
    24481 aaatcagcat ctataattat ttccatgtgt gcattttcta attcaattat attttgagca
    24541 attgataaac caagaccaga gccatctgtt tttctagata tatcacccct tctaaatctt
    24601 tctattaact cttctggctt tagatttagt ttatcatttg aaatattttt aaacgttaat
    24661 attatatttt cttcctttat aaacatatct atatagaccc tagtgttagg catagagtat
    24721 tttagtatat tgcaaattag attctcaaaa actctccaag tttttttacc atctatcatg
    24781 gtaaaaacag catattcagg tgtttctatt ataaaatcta aactgctatt ttgaattttg
    24841 tcttcaaact ctacaatact ttgaattaaa agttctctta tatcggtttt tgttatatta
    24901 aattctacat ctccactact tagcttagat gcttcaaata tgtcatctat taataatttt
    24961 aatctttgtg attttttatc taaaatattt atatagtcat ttatagtctc tctgtcaaag
    25021 tcattatttt ttaacatatc aacatagctt aatatagatg taagtggagt ttttaagtca
    25081 tgagatacgt ttgtgataag ctctgccttc atcctttcat tttttatatt agcatctaca
    25141 gcagtttcaa gaccacctgt tatattattt atacttttgg ctaaagtaga tatttcatca
    25201 tctcctataa cttctaattt atactttaaa ttgccatttt ctatattttt tatatttttt
    25261 gatatgttat ttatgtaatt aaacttaaca gataaaaagt tgtatatata aaaatgaaaa
    25321 actgtgttta caagtgtaaa taaaattact atagctattg cctcagaata atatatatac
    25381 ataaagaata tcaaaacaag atattggata atagcttgt
//
LOCUS       DAELSY010000046        25285 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.046, whole genome shotgun sequence.
ACCESSION   DAELSY010000046 DAELSY010000000
VERSION     DAELSY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25285
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.046"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(52..261)
                     /locus_tag="KPG09_001661"
     CDS             complement(52..261)
                     /locus_tag="KPG09_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966278.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(293..502)
                     /locus_tag="KPG09_001662"
     CDS             complement(293..502)
                     /locus_tag="KPG09_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0966279.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            801..1220
                     /locus_tag="KPG09_001663"
     CDS             801..1220
                     /locus_tag="KPG09_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0966280.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            1448..1903
                     /locus_tag="KPG09_001664"
     CDS             1448..1903
                     /locus_tag="KPG09_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966281.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(2128..2388)
                     /locus_tag="KPG09_001665"
     CDS             complement(2128..2388)
                     /locus_tag="KPG09_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966282.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(2651..3004)
                     /locus_tag="KPG09_001666"
     CDS             complement(2651..3004)
                     /locus_tag="KPG09_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966283.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQAKQDDDITNNFI
                     ANAEIYEMLLSQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGRKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(3021..8198)
                     /locus_tag="KPG09_001667"
     CDS             complement(3021..8198)
                     /locus_tag="KPG09_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome condensation regulator"
                     /protein_id="HBG0966284.1"
                     /translation="MKQNKLLQRGAYFNDKNILIDDFDKRYNDYDFVEFFTGISNSTF
                     GLKSDGNLYACGDNTGFQLGLGKDSSERRMFSKVKIDNVKYVSCGSKHSVAVTKDGFA
                     YGAGTSNVGQLGVIESTVYYEFTKLPIDDVKTVACGYDFTFVLKNDGTLYSAGLNSSG
                     QLGLGDTNNRATFTKVNIDSVKDVVTYNQSVFIIKMDGTAHACGLNSNGQLGINSTLN
                     KSVFNKIEGMDNVKQIACGSSHTILIKNDGTMYTTGYNGVGQLGTGNNNNSIVFTLSS
                     INNVKYASCGNNHTMILKYDNTLFSTGQNNYGQLANANKDVASRNTFAKVNVENIKDI
                     KCGSQFNFLINGSKEIFVSGCNLAGQLGSFFHTTFLYEFSNVQSSNLDNYSGLLVNDD
                     YLYVTKDNSEFLNVKLSDNFQDYKKIELTDSNMFIVMNDGTLYACGLNNYGQLGLGDT
                     VNRSVMTKVDIDNVLDIKGNGNSTFVLKNNGTLYSCGLNSNGQLGLRDEVNRNIFTKI
                     EIENVKDFCVGSNYVIALNHSKEVYGWGNNPYNNIEKTSNYPYKQGISNIEKIAAYDY
                     SVYMINSEGKLYVSGYNYNYQLGKGNNSNQSKALVSQCRTNSTSSTSNGLRTLPKITN
                     VFPFYDGCAIIDEGGYVYLTGYHGYLRTLNSSPSISDYSRYGTFIEATNSNHNTYFIQ
                     ETDFSGIEKVIGMSNNILFFKKGSSYITGYPKTFGSTITGHRSYTSINSESSNLGSNF
                     IIYHSNSKLYGKGIANSGQFGNSTNIDGTSNYDTGLKDIKDIIVKGNTVVVVDKNNNI
                     YVTGMNQNNKLGIGEYNNEPVKKFTNITEQSNSFIFMDDIKEITTSRNTMFIVKNDGT
                     AYATGNNSSGQLGLGDTINRNKFTQINLDNIKKISTSIDGNTTFAIRNDGTLYSTGLN
                     TKGQLGLGDIVNRNTFTKVNIQNVRDVVLGTTHSHAIKDDNTLYSCGENTHGQLGLGS
                     ESNHPDVLTFTVNNITNVRDVYCSDTTTFIVKDTNIAYCCGYNNNSQLGMGNTTDQYS
                     FIKCMENVKEVIPNEINTYIITIYNTAYSTGLNTDYCLGLNSNSNQSSFSEIPISNVV
                     KVAPNRNNAVLLLTSEGDVYTAGKCSNGSGTGSETPEKIKKIASKAKDIGMNYRCGHY
                     VSDNGDLYGTGFNNNGQLGVGDVTKRDTFIKTNTRVKKILPLEYANIAIKDTNDIYIC
                     GLNNYGQLGVGNRYDSRNNDNRIFNYKHMNFVMGDLTSIKNRHNFILLNNKIVIPTTK
                     DIDYGLVLGNLYKGDLYTELPYEDIKEVSISKTHIIILLNDGTMYGCGTNYHGELLQD
                     LSINQVDEFVQINVSDVKHVSCGDNFTYFIKSDDSLWSIGKNSEYQLGIGHNNPVTEL
                     QRITTISSCKEVHCGKNYTLVVTTGNELFVQGYNDKGALGLGSDSENTIIKFFTKALT
                     DIREIKSYGSDHILVLKNDNSVWVTGKNRDVYKIEQPVEFLKEFTIVPISEDVNTVKD
                     VLATDNTLYIISEVGTTNAAIEITEKSISSIKIKIQDPNKDISRIEMLINGESVKSVS
                     DLTTEKISFEVPPDKIKIGENKILFRAYCKGDDLYASLFIFKESTGNSIIKDSYVMIG
                     NRMYKVVNTTSNEQDITITLDRGLEEDLNLGDPIYQLINKTKVQVKINKSDLFKDMKL
                     VEIKKSDSSYQEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(8220..9206)
                     /locus_tag="KPG09_001668"
     CDS             complement(8220..9206)
                     /locus_tag="KPG09_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG0966285.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGDTDILTTDSKDLSGAINEVVKKIENISFDDVISGQIQTDISVLKNSY
                     NKLSEKVLDILIYLELESEVTVDEAGYWYDTLANGNNIVAIEGLKLDLNRKCITGEIG
                     NVIFRDVVLPFSANRVRYIHDMDNNFVETKSSNTYLKEQKDITLSKYSYEIR"
     gene            complement(9207..9905)
                     /locus_tag="KPG09_001669"
     CDS             complement(9207..9905)
                     /locus_tag="KPG09_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBG0966286.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSEMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(9902..10954)
                     /locus_tag="KPG09_001670"
     CDS             complement(9902..10954)
                     /locus_tag="KPG09_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG0966287.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGENNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(10947..11375)
                     /locus_tag="KPG09_001671"
     CDS             complement(10947..11375)
                     /locus_tag="KPG09_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG0966288.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(11375..11701)
                     /locus_tag="KPG09_001672"
     CDS             complement(11375..11701)
                     /locus_tag="KPG09_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBG0966289.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(11717..13246)
                     /locus_tag="KPG09_001673"
     CDS             complement(11717..13246)
                     /locus_tag="KPG09_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0966290.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(13528..13950)
                     /locus_tag="KPG09_001674"
     CDS             complement(13528..13950)
                     /locus_tag="KPG09_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966291.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEVATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(13950..16403)
                     /locus_tag="KPG09_001675"
     CDS             complement(13950..16403)
                     /locus_tag="KPG09_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966292.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASSRVNSLNSSLRKVQTTASSVSSTMAKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKRVEKRPYQGSINLKDMVSSAMARILPKLMLFKNT
                     FWSGVIAIKDMASSIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKTSQKDVDKYYDSLVNMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSSKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(16403..16570)
                     /locus_tag="KPG09_001676"
     CDS             complement(16403..16570)
                     /locus_tag="KPG09_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966293.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(16597..17043)
                     /locus_tag="KPG09_001677"
     CDS             complement(16597..17043)
                     /locus_tag="KPG09_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0966294.1"
                     /translation="MNENGLSKNINIVDLLLNADTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(17157..17585)
                     /locus_tag="KPG09_001678"
     CDS             complement(17157..17585)
                     /locus_tag="KPG09_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG0966295.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(17599..18663)
                     /locus_tag="KPG09_001679"
     CDS             complement(17599..18663)
                     /locus_tag="KPG09_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG0966296.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTEEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(18668..19111)
                     /locus_tag="KPG09_001680"
     CDS             complement(18668..19111)
                     /locus_tag="KPG09_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966297.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPIMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTITLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(19360..19800)
                     /locus_tag="KPG09_001681"
     CDS             complement(19360..19800)
                     /locus_tag="KPG09_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966298.1"
                     /translation="MENKKDILFKETDERLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(19905..20102)
                     /locus_tag="KPG09_001682"
     CDS             complement(19905..20102)
                     /locus_tag="KPG09_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0966299.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            20271..20591
                     /locus_tag="KPG09_001683"
     CDS             20271..20591
                     /locus_tag="KPG09_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG0966300.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            20673..21179
                     /locus_tag="KPG09_001684"
     CDS             20673..21179
                     /locus_tag="KPG09_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966301.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            21880..22635
                     /locus_tag="KPG09_001685"
     CDS             21880..22635
                     /locus_tag="KPG09_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0966302.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(22778..23206)
                     /locus_tag="KPG09_001686"
     CDS             complement(22778..23206)
                     /locus_tag="KPG09_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG0966303.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(23627..23827)
                     /locus_tag="KPG09_001687"
     CDS             complement(23627..23827)
                     /locus_tag="KPG09_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG0966304.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(24010..25179)
                     /locus_tag="KPG09_001688"
     CDS             complement(24010..25179)
                     /locus_tag="KPG09_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBG0966305.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSEDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
BASE COUNT         7929 a         4407 c         2705 g        10244 t
ORIGIN      
        1 aaaatataat acatttctat gtataattta tattcctaag aaatgcatta ctcatgtttt
       61 taattcttcc aacatctttc tatacacaat tgtacatcct tcataattcc cctctttaag
      121 aaggattgaa catgtactag aatatttagt taaagtattt aatctttcca tatttaagct
      181 ttttatatta ctaagtgctg taataaaatc atcaattgct ctacaacact ctataacccc
      241 atctgaatcc aatttgcaca caagaatatt cacctacttt atatactatt tattaatcat
      301 cttctgaata ttctatatta agtaattgat aaattgatga tttatatttt ttacctgttc
      361 ttcttccatt taaaatagct gacaaatagt tttcattaac acctattttc ttcgctaatt
      421 ccctttgagt catatttaat tcaataagtc tttgcttgac ttgcactccc aaaaaaggta
      481 tccatttttt tcgagcatcc atttatcaac ttcctttcta agtgatttat atttactttt
      541 atattacttt agcttacgca ccccctctat ttagtatata tttttaatat gtacacatat
      601 taagtatata aatttattta atactaaata agattttttg attactttgt gctaaaatta
      661 ggatgtaaat tcgtatcatt aagttaatta taaagtcatt ttgaccttaa gtcaatggtt
      721 tttatgtcat tttgaccttt aatttgaaac tatttacatc ttaaaaagtc tttttgacat
      781 taattgaatg gagagtgttt atggatactt taggagaaag aattgtttat ttgagaaaag
      841 caaaaaacct taaacaatat gaactagaag aaatgttagg ctgtgacaac ttaagtaaat
      901 ttgagagaaa cattagaaaa ccaaattatg aaatattaaa atctatagct gagatattta
      961 atgtttcagt agactggcta ttaaatggag ataatctatc acataaatca gatttaattt
     1021 gcgattcctc ctcaaactat ccacttaata gcataaactc taatgaaata aagcttttaa
     1081 acaactttag aaagttaaat gattatgata aagccaaaat agaaggtatg attgaattaa
     1141 aacttcatga atatgaaaaa gagaaggatt taggaaaaat tgagtataac aaaaataaag
     1201 atgagaaaat agataaataa tttttatgtt gcttttgata cgattttaca tcatttcttt
     1261 gttctaatat ttttatttca tttattttct tttttataat tttatgataa aataattagt
     1321 atattttgct ttacttgaat gtaattattt atacaaaagt caaacataat ataaatgtag
     1381 taaattttta gtttcaagta atatctcgtt attataacta aatatttatt aaaattagga
     1441 ggataaaatg ggcttaagag acaaattcgc gcaatcattt gctagatcaa aaactatgag
     1501 tggccctgag aaaaaggcta atgaaataat gggtaaattg cttcttaaga aagctatatt
     1561 acctatcgtt ttaatgttcg tgataataat cgctggagct atgcttaaaa tcaattcttg
     1621 ggttacactt ggcataaatt tagttattgc tgttggtgct ttcttctaca taagaaattc
     1681 tagtaaaaag tatcagaact ttaaacctta tgttggcaat ttgattagtt tagagaaaaa
     1741 aggtaaaaaa gagtacgttg ctattataaa acaaggtaag ttaccagtta agttacaaat
     1801 agcttatggt ggtgaagacc ttgaacatgt taagaaaaat caaatggttc agataagtta
     1861 taatccagat gctaaaatag ctattttggt taatagacaa taatttaata aaaaaaagga
     1921 gttgtcttaa aattgatttt ttctaatcaa atttaagctt aactcctttt tgaattttta
     1981 tatgttattt ttatatatta tttttattac tttttttaat taatatttta aaatataaaa
     2041 tccttctcaa taaaataggg cactatctac aatactactt tatcttttcc taataatata
     2101 taatctggtg ctactgctac tttcatgtta tacaccgttt tgtaacatac cttttatatc
     2161 agtaacatct tccttaattt cctctacatc tgttttcatt attcccattt gaacaagtat
     2221 atttttgttt atttcttgtt gttgtgtaga taattctata aaattttcta cagttttttt
     2281 atacatgtct ctatcttctt ttttctcctg catagtattt ttaaataata gagcacatag
     2341 tatacctatt gcccccagac tactcaattc ggttattaat ttatccatta tagcctccta
     2401 ttgtctaaat tttttatact tatagcttta atttttgtta tattcttaca tcatgcaata
     2461 ttatattttc aggattatca atttaatctt ctgtacttta aattgtaggt ttaatttata
     2521 gattgaataa ataataaata tatatacaat ttctaatata acaaaaaata agtacaaggc
     2581 ttacttatta aagttaagac cttgtacttt acataaaaaa aataactaat acatagttat
     2641 tctaaattta ctattcaact ggtatttcta attctttaca tctaattcta acttgctctc
     2701 taattactgc tggtacttct tctattgttt ttctgcctct taaaattaaa gctacatata
     2761 cttctaccat tccactctca cctccagatt ttcttacact aaaagtgctt atattacttg
     2821 cttcattgac agaactttga cttaacaaca tctcatatat ttctgcattt gctataaaat
     2881 tatttgttat atcatcatct tgctttgctt gacctatact tatgtctgaa atgtctttat
     2941 actgcatttc tacttttcta ccttgtttga tttgttctat tttattttta tcgaatttaa
     3001 acaattttta cacctccatt ttaagcttgt ggctcgttgt tttcagcatc atatctaaaa
     3061 atcatagatg gacgttttat agctgtccat ttatctcctt tttctactat gattttaggc
     3121 tgagcacttt ttatgttggc ttcttctaat tcatagattt cttgataact tgagtctgat
     3181 tttttgattt caactagttt catgtctttg aataagtcag atttatttat ttttacttga
     3241 actttagttt tatttattaa ttgatatata gggtctccaa gatttaaatc ttcttcaagt
     3301 cctctatcta gtgtaattgt aatatcttgt tcattagatg ttgtattaac taccttgtac
     3361 attctattac ctatcataac ataagaatct ttaattatag aatttccagt actctcttta
     3421 aaaataaata aagatgcata taaatcatca cctttacaat aagctctaaa tagtatctta
     3481 ttctctccta ttttaatttt atctggtggt acttcaaagg atattttttc agtagttaaa
     3541 tcacttacag attttacact ttcaccattt ataagcattt ctattctact tatatcttta
     3601 ttagggtctt gtatttttat cttaattgat gaaattgatt tttcagtaat ttctatagca
     3661 gcatttgtcg ttcctacttc tgatataata tataatgtat tgtctgttgc aagtacatcc
     3721 tttactgtat ttacatcttc agaaataggt actatagtaa attcttttaa aaattctact
     3781 ggttgttcaa ttttatatac atccctattt tttccagtaa cccatactga attatcattt
     3841 ttaagtacta atatatggtc acttccataa gattttattt ctcttatgtc tgttagtgct
     3901 tttgtaaaga acttaattat agtattttca ctatcgcttc ctaatcctaa agctccctta
     3961 tcattatatc cttgtacaaa taattcatta cctgtagtta ctactaatgt atagttttta
     4021 ccacaatgta cttctttaca gctagatata gttgtaattc tttgtaattc agtaactgga
     4081 ttattgtgac ctatacctaa ttgatattcg gaatttttac caatagacca aagactatca
     4141 tcagatttta taaaataagt aaagttatct ccacatgaaa catgctttac atctgataca
     4201 ttaatctgca caaattcatc cacttgattt atagacaagt cttgcaataa ttctccatgg
     4261 tagtttgtac cacatccata cattgttcca tcattaagta atataataat atgagtctta
     4321 gaaatagata cttcttttat atcttcatat ggaagctcag tataaaggtc tcctttgtat
     4381 aaatttccta atactaaacc ataatctatg tcttttgtgg taggtatcac tatcttattg
     4441 tttagaagta taaagttatg tctgttttta atagatgtca aatcacccat tacaaaattc
     4501 atatgcttat aattaaatat tctattatca ttatttctac tatcgtatct atttccaaca
     4561 cctaattgtc catagttatt taatccacaa atatatatat cattagtatc ttttattgct
     4621 atatttgcat attctaaagg aagtattttc tttactcttg tattggtttt tataaatgta
     4681 tctctttttg ttacatcacc aacacctaat tgtccattat tattaaaacc tgtaccatat
     4741 aggtctccat tatcacttac ataatgtcca catctataat tcattccaat atcctttgcc
     4801 tttgatgcta tttttttaat cttctctgga gtctcacttc ctgtacctga accattacta
     4861 catttgcctg cagtatatac atccccttca cttgtaagta aaagtactgc attatttctg
     4921 tttggagcta cttttactac atttgaaatt ggaatttcag aaaatgaact ttgattgcta
     4981 ttactattta gacctaagca ataatcagta tttaaacctg tactatatgc agtattatag
     5041 attgttatta tataggtatt tatttcattt ggtataactt cttttacatt ttccatacac
     5101 tttataaaac tatactggtc agtagtattt cccataccta gttgtgaatt attattgtat
     5161 ccacaacaat atgcaatgtt tgtgtccttt acaataaatg ttgttgtatc tgagcagtac
     5221 acatctctta cattagttat attgtttaca gtaaatgtca atacgtctgg atggttgctt
     5281 tcgcttccta agcccagttg cccatgagtg ttttctccac atgaatataa tgtgttatca
     5341 tctttgattg catgcgagtg agtagtccct aaaacaacat ctcttacatt ttggatgttt
     5401 actttggtaa atgtatttct atttactata tcacctaatc ccagttgtcc tttggtattt
     5461 aatcctgtgg agtatagtgt tccatcattt ctaattgcaa atgttgtgtt accatctata
     5521 cttgttgata ttttctttat attatcaagg tttatctgag tgaacttatt tctatttatt
     5581 gtgtcaccta atcctaattg tccagaacta ttatttcctg tggcataggc tgttccatca
     5641 ttttttacta taaacattgt atttcttgat gttgtaattt cttttatatc atccataaat
     5701 ataaatgagt ttgattgttc agttatattt gtgaattttt ttactggttc gttgttatat
     5761 tcccctatcc caagtttgtt attctgattc attcctgtta catatatatt gttattttta
     5821 tctactacta ctacagtatt tccttttaca attatatctt ttatgtcttt taatcctgta
     5881 tcatagttac ttgtgccatc tatatttgtt gaattcccaa attgcccact attagcaatc
     5941 ccttttccat ataacttgga attactatga tatattataa aattacttcc taaattagaa
     6001 ctctcagaat taatactagt ataacttcta tgtccagtaa tggttgagcc aaatgttttt
     6061 ggatatccag taatatatga acttcctttc ttaaaaaata atatattatt tgacatccct
     6121 attacttttt caattccact aaaatcagtc tcttgtataa aataagtatt atgatttgaa
     6181 tttgtagcct caataaaagt tccatatctt gaataatcag atatacttgg actgctattt
     6241 aatgttctta aatatccatg atatcctgtt aaataaacat aacctccttc gtcaattatt
     6301 gcacaaccat cataaaaagg aaaaacatta gttattttag gtaacgttct aagtccattt
     6361 gatgtagaag atgttgaatt tgttctacat tgagatacta atgctttgct ttggttacta
     6421 ttatttcctt tacctaattg ataattataa ttgtatccag aaacatatag tttcccttca
     6481 ctgtttatca tatatacaga ataatcatat gctgctatct tttcaatatt acttattccc
     6541 tgcttatatg gataattaga agttttttct atattattat aaggattatt tccccatcca
     6601 tatacttctt ttgagtgatt taaagctatg acataattgc ttcctacaca aaaatccttt
     6661 acattctcta tttctatttt tgtaaatata tttctattaa cttcatctct taaacccaat
     6721 tgtccattac tatttaaacc acatgaatat aatgttccat tattcttaag cacaaaagtt
     6781 gagtttccgt ttccttttat atccaaaaca ttatctatat ccaccttagt cataactgac
     6841 ctgttaacag tatctcccaa tcctaactgt ccataattat ttaaaccaca agcatacaat
     6901 gtaccatcat tcataacaat aaacatattg ctatctgtta actctatctt cttataatct
     6961 tgaaaattat cacttaactt tacatttaaa aattcactat tgtcctttgt aacatataaa
     7021 taatcatcat taaccaataa acctgaataa ttatctaaat ttgaagattg cacatttgaa
     7081 aactcataca gaaaagttgt atgaaaaaat gaaccaagtt gacctgctaa attacagcca
     7141 gatacaaata tctcttttga accatttatt aaaaaattaa attgagaacc acatttaata
     7201 tcttttatat tttctacatt aaccttagca aaagtatttc ttgatgctac atctttattg
     7261 gcattggcta gttgaccata attgttttgt cctgtactaa acagtgtatt atcgtatttt
     7321 aatatcatag tatgattatt tccacaagaa gcatacttaa cattatttat actagaaaga
     7381 gtaaatacaa ttgaattatt attatttcct gtaccaagct gaccaactcc attatagcct
     7441 gtagtataca tagttccatc attcttaata agaattgtat gactactacc acacgctatc
     7501 tgttttacat tatccatacc ttctatttta ttaaatacac ttttatttaa agtactatta
     7561 attcccaact gcccatttga atttaatcca catgcatgtg ctgtcccatc catttttatg
     7621 ataaatacag attgattata agtcactaca tctttcacac tatctatatt tactttagta
     7681 aaagtagctc tattgttagt atcacctagt ccaagttgac cacttgagtt taaacctgct
     7741 gaatataatg ttccatcatt tttaagcaca aatgtaaagt cataaccaca tgcaacagtt
     7801 tttacatcat ctattggtag cttagtaaat tcataatata ctgtagactc aattacacct
     7861 aattgaccta cattacttgt tcctgctcca tatgcaaatc catctttagt tactgctaca
     7921 ctgtgttttg aaccacaaga tacatatttt acattatcaa tttttacttt actaaacatc
     7981 cttctctctg acgaatcttt tccaagtcct agttgaaaac ctgtattatc gccacaagca
     8041 tataaattac catctgattt taaaccaaag gtactattac ttataccagt aaaaaattct
     8101 acaaaatcat aatcattata tcttttatca aaatcatcaa tcaatatgtt cttatcatta
     8161 aaataagcac cacgctgtaa aagtttattt tgcttcatta ttagtactac ctcctttatt
     8221 tatcttattt catatgaata tttacttaga gttatatctt tttgttcttt taaataagtg
     8281 ttactagatt ttgtctcaac aaagttatta tccatatcat gtatatatct aactctattt
     8341 gcactaaatg gtaatactac atctctaaaa atcacattac caatttcacc tgttatacat
     8401 tttctattta aatctaactt aagcccttct atagctacta tgttatttcc atttgctaat
     8461 gtatcatacc aataaccagc ctcatctaca gttacttctg actctaattc taggtatatt
     8521 aatatatcta gcactttttc agataatttg ttatagctat tttttaatac tgatatatca
     8581 gtttgtattt gaccacttat aacatcatca aaagatatat tttctatttt tttaactacc
     8641 tcatttatag ctcctgataa atctttagaa tctgtagtta atatatctgt atctccaatt
     8701 tttactttta tttcttttat atttttatca aacttatcat ctaaatcttg aatatccttt
     8761 tgagttacaa aaaccaacgt cgggtctact tctaatgtaa catttgaagt attggacaat
     8821 tgtaaaatca tttttacaac caaatcttta atactgccat cttcagcacg tggcttatac
     8881 gtctctggat acttacctat agctattata ttattctcat tatccagaac agcagcttct
     8941 cttatcataa atccaccaac tgttcctggt aaaatagtct ctataactat ccaattagga
     9001 tttttttcat caattcttag agaatttata tttccttccc aaactacatt ctttaaagca
     9061 gtttgttcct ctgttggctc ataatattct ccattactat ctccaacttg aaacttggtt
     9121 aatccaacaa gctctcctag tgcagttgca tttgctattt tttctctacc aattttggtt
     9181 aatattatat aaaatttttc ttccaattat accacctccg atttaggata aactactaca
     9241 ttttctacgc ctgtatcatt tgcaatagct atattaatct tacaattatt ttcaatgcat
     9301 ttaatgctta aaggataaac ctttatttct tctctactta acattccagc tccacagttt
     9361 acaacagcac tacattttat aattggttca aagtatggat gaacttttac ctcttcacta
     9421 cataccatac ctccaccaca atagaccata cttttatcta gtattattgg ttctaaatag
     9481 tgtaataagt gagcaggttt tactctttca atcatctcgc tacaatctaa aagagtttta
     9541 tagtcacagt tatttgctat aaaactcaat acgaatgtaa attcatcact ataaactttt
     9601 atatctgttt ctgattttgt atatgcctca catatacttt ttataacttc aatagtacta
     9661 gtacctctgc ttctcatttt agcttttata ttgctacgtc ttgtgtcaaa atcaagttct
     9721 tttttttcaa tgcagagtat gtcttcccac atatctaatc cccatgtcgc tgtatcaaca
     9781 aataattggt taaaggtatc atcaatatct tctttaagaa tatttaattc tgtatcgtat
     9841 gcaccttgta tctcctctac aatgtaattt ctatcaaatg atggtagttt atcaattagc
     9901 ttcattgatt ttccacctca ctaaaattaa cagttgttac actaggtatt ttatcttcat
     9961 taatagttat attatctgta cttccattta taagtagatt acttaaatcg tgtacaccag
    10021 tagtatttat aagtattccc atgacttttg tatagattat ctctcctcta atatctctaa
    10081 agtatgtatt tatactttct aggaaagatt cttttacatt gtctaatgta tatccatctt
    10141 ctagtttcat tactgcactt atacttattt ctattggtaa tggtgtcaca actgttatag
    10201 ttggtccaat aggcttctct tcatctatat gttgctgaca cctttcaatc gtttctgtat
    10261 caacagcttg attattttcc ccaaatatca agaccttaac tgttcctgga ccatcccatc
    10321 ttggataaac cttaacatta tagactccat ctacttccaa agcccattct tcatagtgag
    10381 ctttatttcc acttgtagct tgatttcttt ggattttata aaatctttct tttagttctt
    10441 catctgtctc tatatctgta ccaccttcaa aacttcttgt gttagtaatc tttgttactc
    10501 cagatatatt atcaactagt ttaaattcac aatttgtagg taaattatat ttcttaccaa
    10561 cttccagagc ttgaactggg ctattgtcac cttcttcact accaatagtt acatctttta
    10621 ttactacaaa tagtaaatct ctatatgata ttattgtgcc attatttatt acagttcctt
    10681 tctctccaat aaattcaact tctccatttg actctgtacc taactttcta tatacaccaa
    10741 attcattgac tcttttatca agaaactggt tatatgtgtc ttgtataaaa gccattttat
    10801 gtatctttga aagttctaga tatatcttcg aaagttctag attatttcca gataccatgt
    10861 tgtttagaaa agaaccttct cctttataaa tatcaagatt aatattttca agagttctat
    10921 tttttattac ttcatatgtc tggtcactat acatttattt ccacctcccc atagatagtg
    10981 gatactttta catttgcact caatacgtct ctgttaaagt tagcactttt tacgtttact
    11041 tctaatatat atggatttat tagaagggcc tcttttatga atctactagc ttcactttct
    11101 gtaagacctt ttgtatattt ttgtcctatt agttctgaaa gctctgtccc atattctaaa
    11161 gagtatatct catgttcata tctatttgtc tttatacact tgtatatcca aacttttaag
    11221 gcttcttttt tttcaattat tttaaagtca cctttttcta aaataggttc atctttttca
    11281 aaatcccatg ccacttcacg aaagattggc aattcttctg ttttaggtaa gatataatcc
    11341 tctgggacac ctataaaagg aaatattgta ctcattataa gctcaccaac ttacttacaa
    11401 cagcaaattt ttcgcctatt ctaaacatta ttaccatatc cccagattca aagttatcta
    11461 taaaaggatt ttttacttca tgctggtgtt cttgatttgt ttctgtgtca aatgtttcta
    11521 gctgtctatc aatcatccaa ctatctatca aaatatcttc tttttctaat atgatgttat
    11581 ttgtctctat ttttaaatct ggtaatttac ttttaatttt tccaataaaa aaagaaggtt
    11641 gattatgata ctttccttct tctcttatta ttcctataaa ttcatttatt ggattagcca
    11701 ttatatcacc atccttttat aatactctcc tagctgtatt aaaatctctt ctactactta
    11761 acttacttat tttaactaca tcaccttttt gtggagcatg taaaaattct ccattaccta
    11821 tatataatcc aacatggctg actggattat gaaaaaatac taaatctcct gcttgaagat
    11881 ttttttgttc tactttcttg ccttttttag attgttgatt tgacgttctt ggcaaactaa
    11941 cattaacttt tttaaaacag tagaccatta aaccagaaca gtcaaaacta cttggtccat
    12001 ttccacccca tttataaggt ttacctagat gtttttttgc ctctgatatt actattttag
    12061 ctttatcaga tacattacca gaataaccac caataataat ttttcctttt cttcttccaa
    12121 atttactagc ttcttcaaca tttccaaata aaatatctat atggtatgtt ccattttttt
    12181 ctatagttat tgcaggtcct acgtcattta ctttatagac tccatcacga ccagaaactc
    12241 ctgtaattac ctgtatttca tctccatagt tcaataaagg atgtttattt aaaaactctt
    12301 ttgtataata agtttgctca tatttaccaa ccataggagc agcgcaagtt tttttagatg
    12361 ggtcaagttt tttctttcta caatctgtat ctccaccttc ttcttttcta ggacagtaag
    12421 ctgtaaattc tgctgtaaac tcttttcctc ctgcataatc ttctccccct aaattacttt
    12481 cttccttagg ttcatcctgt cctgctgact tttcatccat aagattttga aaattaagtt
    12541 caagctcaat ttgatattct ccattttgcc aagtatgttt gtctgtatct atataaaata
    12601 gtcctacaag ctttgtataa gaatctttaa cttttactcc tctaccagtt atacaactta
    12661 catctccata acctttaaga gaacagcttt tttctatccc attaaactcg ctatcaatat
    12721 ctacatcttg attttcttgt tgctgaatta ctttttgcat tattacattt acttccttaa
    12781 aaatttcatt atctattttt tcgctaatct tgcttccata ctggtctacc actattactt
    12841 tattttttac attttccatg ctctccgaaa aggtggtatt tataatatta aatccctctt
    12901 caaacataac acttaatgta acagttcctt tttcaataac attaaactta tctaaattag
    12961 cctctatcat atactttttc tttgtctttt tacttgcctc tgtatatgca ctcattattg
    13021 tgtcataacc atttactcct ataaacatct ttgtatactt gacattggtc tttgctattg
    13081 ttccaactga aagcctattt tcagcaaata cttgctttgc tatgtcttca actaacttat
    13141 ctttaaaatt gtatgacact tcactttgtg taagtaaaaa tcccatatct ttagatacaa
    13201 aactaatttc attactgctt gaatctttag acctattaat tatcattcct ctaaagagtt
    13261 ctttctcatc tacatagaaa cagactgtac tagctattgg tatatcgatt tgttggaaat
    13321 ttacatcaaa tgatgattgt attattgaaa actctagtgt ccttgatgga gatttataat
    13381 cacctgacca tgataccttg tctactatgt cagttatgtc atatatactt ccgtttttta
    13441 tgtgtaccca aatcttaata ataaaaaatc acctgccttt tcatttaatt ctatcagcag
    13501 ttacttacaa cttattatct ataatagtta ttgttttgga gttacttttg gtatctttat
    13561 tcttttatac tcttttaaat ctaatgtaaa atatacatct cgagtcccat ctctttcttc
    13621 ataactaaac ccttcaatta tgacttccat atttatattt gtttccgtaa ttgtaaatct
    13681 taatataaaa ccttccttca tccatttttc tatcttattt acacaatcat atggtgatgg
    13741 aaaacctgta tagtcacaga aagtgtagtc tttatttgga aaaaaacttg atattgaaat
    13801 gcttttaaga gctacaccac caaaagttgc tacttcacct attttgagta tactagaaga
    13861 gtttacagca gcttttccat taatactaaa ggaagatgga aatactggaa atctaaatct
    13921 atcttctgct tgtctaagcc acatttccat tataagacac cccctaactg tgccaattgg
    13981 atacttgcaa ctaattttga tgttatcttt tctatatcag cttcttctct aatcactatt
    14041 gtatcagcta acttagctat gtttatattt ccaccatttt ttctgcttct atattgattt
    14101 gcttcttgtt tagttaaaac catttcacct tcatggagtc tggtttgata gtcattataa
    14161 ggaacatagt tcaatccaaa tgcattatta cctttcttac ttgaataagc tttaccagaa
    14221 tcaccagcct ttgttccagc atatggatta tctttgtaat tttttttact cttttccact
    14281 agatttaaaa ctgttcctgt gatgcttcca ttttttatct tattaatcat agtttgccac
    14341 catttgcata tatctttaac tattttacat actttatcta atatgttaaa aaaagctccc
    14401 aatattggct ctataatttt ccatccagtt tccaacagag gtcctaaggt ctcccataca
    14461 gttttccaaa taacacccag tgtctctata aaattgttta tttgaggtgc atattcacct
    14521 aaaaaagtaa aaattcttgt gataatagga ctaacagctt caactacagg tccgattagt
    14581 tggaatgctg tagttactat tgtaccaaat atacttatta atccttgtgc aattggtgat
    14641 ttattgttta tactatctag taaattattt actaagttta ctactggaag tagtgaatct
    14701 attaatgctg taaaatcaat attttgtaat agtgtacttc ctgcgttaac aattgctttt
    14761 ggtgcattcc ctatcattgt tgctgcatga ttttcaaaac gttcttgctt attttcaacc
    14821 atcattttac gcttttcagc ctcattagaa attcgatatt gagtagaatc agaaaactct
    14881 tttccatttt ttacacttct agattgcatc tggtcatatt tgttgccaat aattatctta
    14941 ccattcatta cattgcctat tttaccagcc attttttcag ataagccagc taaattccct
    15001 aatccacttt ctataaaagt ttttattttc tttagtcctt gacctaaaat atcatcaaaa
    15061 ggtttaacaa tactcttcaa accattgtta atgcttgttt gagctcctga tattatacct
    15121 ggtattgtat tactcatttc tttagctaac cccatctgct ttacacttat gccttccaat
    15181 gcttcatcaa aagttttata attttctcct accagagtat taagagattc catatttcct
    15241 tttgctgcac ttaagaaagc tgctgataca tcttgttcac tacttgtatt catatttaaa
    15301 gctctaacat ctaccatagc ttgagttata tctttttctt ttttgccacc agtaatatta
    15361 ctaatcattt tagctttagt tcccattgca acaacatctt cagggtcaaa aggcgtatca
    15421 tttgccatat ttactaaact gtcataatac ttatcaacat ctttttggct agtttttcca
    15481 gtatttcttt tcattacact ttctagaaca actttctgat tttgttcctg accaagtaag
    15541 tcaacaccct ttttcacagc gacacctata gtagcaccat ttgtcaaatc agatatcttc
    15601 ccttttatcg aaccaagtat tccacttgcc atatccttta cagctattgc accactccat
    15661 accttacctg caaacaatct caatttggga aatactttac ttataatgct acttgccata
    15721 tcttttatag ctattacacc actccaaaaa gtatttttaa acaacattaa cttaggcaaa
    15781 attctagcca tagcactact taccatatct ttaagattaa tacttccttg ataaggacgt
    15841 ttctctactc gttttaattt tcccaagata ctacttatca ctctcttagt catatctttt
    15901 gcttttatta cgatattcca aatcttgcca ccaatctttt ttaaatttcc aagtaattta
    15961 tttttagttt tttctacttc atcactttta gctttaaata ttaatataat cataatattt
    16021 gaattaaact catttttctt ttgaatcaac ttattaaaat aatcaataat catagctata
    16081 gttattgcta gtctcataat acttccattt aaacttccaa taactcctga aacagcattt
    16141 atgctagaac ttaattttgc catagttgaa cttacactac tagcagtagt ttgaactttt
    16201 cttaacgagg aatttaaact gtttactcta ctacttgcac ttccaataga attttttatg
    16261 ctactagcca ttacattagt ggaaagtgaa acagccacag ttgctctcct aaaattatca
    16321 gttactgata taatttggtt catttttggt gtgtaattat ctgtaagata catatttata
    16381 ccttcttctc tcacatttcc cattacatac cacctcctgt caaaggacac actccatatc
    16441 catgtttcat tttttcaact tcttcctcaa tttctaaggc gaaaaaagct tcaataagct
    16501 ttaattcgcc tttatccatt gcataaaaaa gggacggtct tatacctttt ttcttccaat
    16561 aatagtacat tattgtagtc ttggtatccg tccctattag ttttttacct cttctatcac
    16621 tgcattttta ccataaccca taacatcttg aagtattcta tataggttgg taatttcacc
    16681 tggtaataat agttttcttg ccaattcttt tggtgttgga gccttaaatt tatccattag
    16741 ttccttattt ttaaataaaa gctttccatc caaatcacaa acaccttcta taattgtaag
    16801 catctgcatc tccggtaagt ctatatccgt tttcatattt tcatcaattt taagacaagt
    16861 attttgtatc tcttcatctt tacttattgt taaagctcta cacattactt taaattcctg
    16921 cccaaatata gttgataatc tcttaagttc aactatagta cttggtcttt ctaagttttc
    16981 tgtatctgca tttaaaagta aatctactat gtttatattt tttgataatc cattttcatt
    17041 cattattatt tgctccttta tcaatcaaat tcaatcaact aactttttta cttattaact
    17101 acctaaattt attaagtata tatctactaa atatcagtat aaaaccaaat ataaaactaa
    17161 attatatcaa gaaaatcata ctcagtaaat gtgaaaggtg cttctatctc tccttttact
    17221 ccaacctccc aatcagctaa agttaaatca tcaaatgcta tattttttac tgctattctt
    17281 tctgctccat aagaatctgg gtcatttaat ttagatatag caacaaatct aggttcagaa
    17341 cctctcttta tcttttctcc aattaacttg tgcattcttg aactaacatg gtatagagtt
    17401 attgaacctt ttcctttata tcccatatac tttgtatcag tacccatttg acctgctatt
    17461 ataatatcct cttttgtaaa ttccatcttt gcttgaaact tctttacttc tgctactttg
    17521 tttccatcaa gccaaagttc tccccaagta ccactcatta catttctagc ttccatattt
    17581 gccatattaa taatcctcct atatttctat tgataaatct atatcttcca tagcatcaag
    17641 tacttttatt tttgctttta aaaatacttt agaacctgtg ttagcttctt ttatttcttg
    17701 taatgtcata taagataaat ctactccttt tgattttaaa tacgattttt gtgcttcaaa
    17761 atctatttca acagtagaat cagattctat aagtgctgac ttttctaatt cttctaaata
    17821 actttttata gctactatca ataaacattt gttgtcataa ctgttagtaa cctttcctat
    17881 atagtcatct attatcacct ttcttatgtc actatgtata atatctaaag tgtcaactat
    17941 ttttattttc tggaacattt ctcctttttc ttctgttaac tcagttaaag aatttactcc
    18001 tcttgcaatt cttatagccc ctgcttcctt aataagtata agctctcctt tattaaccct
    18061 tgattctgca tcaactttcg tcatcttagg tatatcaact acatcactaa gcttagtata
    18121 agttactgat tgacttaaag gtgttcctgc tataagtcca gccaccctac ttgtaaactc
    18181 atcaacactg tatttctttt ctccaactaa cacatcttct gtagtaaaat taattatccc
    18241 ttcatgattt cctacaactt ttcctaatac agctttaacc ttaaccttat ctatatctct
    18301 aagtttaatt atccaatttt ttatagcagt cttatcagct tctactgctt ttggcataca
    18361 tagataatta aattccttag tctctaaaaa atctaatgca gtttgaatat ctgcttctcc
    18421 ttctattaca taaactaata atttatttgg agtgttaaca tttcccatca aagctaaatt
    18481 tatatactct ttattttcag cagataaatc aactggtata tcctcttttt catgtatttc
    18541 attaagacct agtgccttag catctttaag caccattgca attattcctc tagctgaacg
    18601 ttctttaaca gttgtagcta gctccttaaa tgatatgttg atacttggta atcctatagc
    18661 catactatca ctctcctaaa tttaaattga tttcttccat attttcatat actacatctt
    18721 cttcatatac actatccaga taatttagtg taattgtaaa gttcaataca taaattgact
    18781 cttctttttt aatactttgt tctagctttg ttatatttaa ttttctgtct gttaccttta
    18841 tatttctgtt gaataactta tttaattcat ctgaaatttc atacattcta atactctttg
    18901 actgtggttt tggtaagtat tttatagaga taacaatatc tttattattc gtatttaaca
    18961 ttataggtgt acaaataact gatagtatac ttacaaaaaa acaagaccct tcagagcctt
    19021 gcacactttc atcatcaata aaaatacttt cagtaaagtt ttttttgaga atctcaataa
    19081 ttgtttctaa tatttccttg tatttaagca aataatcacc acactatcac taaatattct
    19141 tgcttaatct gataatctta ctataaatga tataagtcat ttttaaatat acatctcatt
    19201 tattcctgtc aaatatcttc tatattttta ggtaatatta ttcttttcaa tttatcacct
    19261 ccttcaatat ggatttcttt cacaataaca ttatctcaca tattttattg cctagtgttg
    19321 caatagtgta tataaagtgt tgtaaagttg tcatattttt tataatatct cactcaattt
    19381 aaatatcatt tttttcttta aattgtagca agatgttcta tctaagtgta gtttcataga
    19441 aatatatgtc atattgtttt ttgtcttact attataaaaa agattaaaaa attccatttc
    19501 tatatcattt agacatgtta gagcattttc tatcttctct ttttctattt ctatatcttc
    19561 tttttccatt tttaattttc ttaatctttc ctctcttttt aacacctcat tttccacaga
    19621 agaacttatg ttatatgtgt tactagtctt ttctgtatat accattgctc cacatccaga
    19681 gtattcattc tcacatcttt ttatgtccaa attaatattc tttatcttta tatccaaata
    19741 cttataatta tgtaatcttt catctgtttc tttaaataat atatcttttt tgttttccat
    19801 agtacttctc cttttttata tacttattat tagcttcaaa tttacaataa tctctttagt
    19861 gcacagtttt ttactaatct cacaactttc aagaacatat tgttctataa ctgtataaaa
    19921 aaaacttctt ctatattttg ttctaaaata ctagaaatct tcttagccat aagaagacca
    19981 ggatttttta tacctctttc tatttgagat atatagtcat ttgtaattcc agcctttctg
    20041 gcaagttgaa tttgtgtcat acctttgcat tttctataat atttcaagtt atttttaaac
    20101 attgttttat acctccatta ccattaagaa tattttgttc ttccacactt agattatata
    20161 gaatcatttg ttctttatca atagatttta gaaaaaaata ttcttttata ttttattagt
    20221 tttatttttt agaacaattt gttataatct atacataagg tggtgttata atgtttgcca
    20281 aaagattaag agaacttaga aaggaatttg gattgactca aagagaactt ggagaaaaag
    20341 taggcgtttc ccaaagagtc ttagggtact atgaaacaga aaacagattt cctgatgaac
    20401 acattttaaa caaattggct gatgtattta atgtatcagt tgattacctt cttggaagaa
    20461 ccttagttaa ggaaaatatt gatacagtag ctgcacatag aaaaaatcca catgaagagt
    20521 tacctgaaga agcacaagaa caacttaatg attatataga atttttacta aataaatata
    20581 aaaaaaaata aaccatatga tgagcagtct atttttctgc tcttttaata taatttaaag
    20641 gcaaacatac attctatttt agggggattt ttatgaataa tttggataag ctttttgaat
    20701 tagcttctca agaagagata ataattcatt ataccactta tattgcaggt gatttagaag
    20761 ggctatatat aaacaaacat ggtattaaaa ttatatcatt actcagtaat ttaaaacaaa
    20821 actcaaaaaa attgacatcc atcttagctg aagaacttgg acatcatttc actagccttg
    20881 gatactatgt atcctcttat aacgattatt atacaaaaat tattatagat aagtgcgaaa
    20941 ataaagcctt aaaatgggct tgtgagttct taattactga agaagatata ataaacataa
    21001 ttaattctgg catcacttgc gtttatgaaa tggcagacat acttaatgtg gatattactt
    21061 tctttcaaaa aagattagaa tttttatcat taaaaaaaca gtctttacaa cttggaaata
    21121 ataaatattt aatattaact aatttgccgt atttctacat atttgatcca atttcataat
    21181 aaaaatagta gtaattttaa ctggcttaac agtaaaatct atatatttat tttaacaaat
    21241 tttggtttct ataaattcaa tattaatata taataatgat atcatattat ttaaaattct
    21301 aaaaacaaat atatgctttt gttaagaaag gttgtttgat gaatgtataa agaaaagttg
    21361 tttgataatt attttaagtt cttagcacta ttattttggc caattatgtg gtataagtgg
    21421 atagtaattt caaatggtac tcttgaaaat atgttattta ccatatatgc aatcgtcgca
    21481 attattttca tcattttata ttcggtgttt atgataaaat ataaagacat tacacaaata
    21541 gatttctttt acaggataag tactctactt gcttttatat ttacactttt tagcttttta
    21601 atatacccaa agagtttatt tttcttgtat ttaaagatta tttttactgg aatatacctt
    21661 tattattcaa tagtaaaaac tcttaaattt aaagatgatg aaggtgtagt gggtattatg
    21721 agctcacttt tattaattgt aataactttg ttctactaaa tttaattcta tttgacttaa
    21781 tatggcttta aatattagac atctacatta ttttctgata ttatttaaga tggtagtctt
    21841 atattaataa tgaaatacac tatgaaggag ttaactatta tggaaaaaaa agttttagca
    21901 acagttggag aaaaagagat aactaatatt gatattgaaa atgcattaaa atcattagac
    21961 ccttatcaag ctatgcaatt taaaactgaa gaaggaaaaa aacacttatt aaatgattta
    22021 gtgaatcaag aattattctt cttagatgct aaagaagaaa aattagatga agaagaaata
    22081 tttaaattag aaatgaaaaa gatagaagaa aatgtattaa agcaatttgc tataaataaa
    22141 gttttatcta gtgttaatgt aacagaagat gaaaaagtaa aattctttga agctaataag
    22201 agctctttct ctaaaccaga aagtgcaact gctaaacata tattagtaga tagtgatgaa
    22261 aaagctaaag aaatacttgc tcaaataaaa tcagaagaaa tatcttttga agatgcagct
    22321 cttaaacatt cttcttgccc atctaaagat atgggtggtg atttaggaac attcggtaga
    22381 ggacaaatgg ttcctgaatt tgaagaagct gtgttctcta tggctaaagg tgaagtaagt
    22441 gaaccagtta aaactcaatt tggataccat ataataaaat tagaagacct tcaagaaagt
    22501 acagagtcta catttgatga agttaaggct gaagttgaaa aaagtttatt atatcaaaaa
    22561 caaaatgaag tttatggaaa taagataaat gctcttaatg ctaagtacgg aaaccttgta
    22621 aaatacaacg attaatcata aggtcatata taaaaaagct gtcttttcac aatatttatt
    22681 agaaaagaca gctttttata ttaaagttta aatattcagt ttcatattat cattctttta
    22741 ttgtaaagaa ctcagcttaa aagtttaaat aagattttta attatcatta gctaaatttt
    22801 tatatccacg tattatctca taacagtaac ctattacatc tcttcttttt attatcccaa
    22861 taaatgtgtc ttggtcatct ataacaggaa tgaaattttg atttaaagat tttataacta
    22921 aatcttcaat atctgcgttg atggaaacag gagaattatc catttttcta tttatatctg
    22981 tcactggaac atcttccaat ccctttaagt ccagatttaa atcgtttttt aatgtccata
    23041 aaaaatcacc ttctgttata gttccaacat atttaccatc cttacttatt attggaattg
    23101 cagagtattt gtgatattcc attttttcta gtgcttgcct tatagtgtag tcttcatata
    23161 tatatgctac ttcacttttt ggtgttagaa agaataatat gttcattaca ctacccaccg
    23221 ttactgaaaa cttataaata ttactcaata tttataatgt tacttttttg cagtaacttt
    23281 ctcctttctt attttctaag tattcttaat actatacgct gtaacaagcg tttttccttt
    23341 gctcatttta atcttattat gctttattaa tatttttata tggtctatca gtatcactta
    23401 ttctcttttt agaaaattta ctaaaaatta attgacttaa acttagccat aataatttta
    23461 ctctacacta ttaattttaa catatttatc tcattattaa tgattttatt ttaataattt
    23521 taatttattt tgctattaat tttatttaca aaaactgaaa aaaccgctaa atgattagcg
    23581 gcttatctct catattacta gaacactaat atatatgaat ttttaattat aatctaacta
    23641 cgttagcagc ttgaggacct ttagctccat ctactacttc gaactcaacg ctttgtcctt
    23701 cttctaatgt tttgtatcca tctccagtta tagctgagaa atgtacgaaa acatctcctt
    23761 caccttctac agatatgaat ccaaatcctt tttcgttgtt aaaccattta actacaccag
    23821 ttttcattaa taaatcctcc taaaaaaaca caaatattta gctactcttt tctgtaacct
    23881 tattactact ctatcatata tagctatgtt tgtcaattat tattattttt atacattaat
    23941 aatcaatttt tttactttat aattttaaat gattattatc ttttacactt tatttttctt
    24001 tataattttt taataattta tttttattat atggaatctt atggacttat cactccagcc
    24061 taaatcccat ggcaccaaca ttctatcagt attatggcaa gttaccaaag gatatcctct
    24121 tgagtctaat cctgtcactg tcgaaacatg tgttattctg cctttcttct cataaccaac
    24181 aaaatccccc ggacgtaaat tataagcttc tttgtatact tcttcatagg aacctttagc
    24241 aattaatgaa cctcttccac tatacagtgc ataattttta aaagcttgtg catttaccca
    24301 tgcttttgta cctgctctct cgctataatt ccaaattgag ttctttttga accttccact
    24361 ttcaaacatt atctgtgatg caaagtttgc acaatctcca ccttctggat tataatccct
    24421 atagtttgga ttaaatttca taccatgttc ttcatcagct gcggctccac agtatttatg
    24481 tgcataatct atagcctttt tttgttcatc tgtaagctgt aactctacac catcttgttg
    24541 cccaatatag tttcttatat cctcagattt tatattttct aaatttaaag agtctgcaaa
    24601 agggtctgtg taccactctt tagttatgac atattgatta tctataattt ttacatttag
    24661 ataatgatat gtacctattc taaaggcatt ttctttgtcc ttttgatttt catatgaata
    24721 cttatattca gtcgatactg aacagattat accatagagg tctttttctt tttcttttat
    24781 ttttttaatc ttaacccttg tctttatgtc tttaaatttt acaccttgct tactagacca
    24841 gttttctaaa tacttcatct tttttacttc attttcataa gcccacagac caaatttttt
    24901 atccgtctca taaagacctt ttaaaatgtt ttcatttttc tctaatattg ccttatttct
    24961 gtagtcaaat aagttttcaa gcaatagttt atattggtct tttacatcat caccttcaac
    25021 atttgcagat tccatgactt tttcttccat atttttcata cttaaatcat tgattaaaaa
    25081 tacacctgct gtagtagcta aagacaaaaa tacaaatgcc attatacctt tgatgtactt
    25141 attcttattt ttcagtattt tcaaagctac acctcccaaa tagtacacct tataaatgaa
    25201 attacaaaga agattctgtc atatacaata attttcatat ttaataattc tctattttat
    25261 tatgtccaat attaaaaatt aattg
//
LOCUS       DAELSY010000047        25004 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.047, whole genome shotgun sequence.
ACCESSION   DAELSY010000047 DAELSY010000000
VERSION     DAELSY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25004
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.047"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            9..1865
                     /locus_tag="KPG09_001689"
     CDS             9..1865
                     /locus_tag="KPG09_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG0966306.1"
                     /translation="MDIHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYRGGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIIDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALINKIFESDKGVVENKKVVTFELNVSINDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSENNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    1948..2188
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2254..2489
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2574..3791
                     /locus_tag="KPG09_001690"
     CDS             2574..3791
                     /locus_tag="KPG09_001690"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG0966307.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRSIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            3905..4399
                     /gene="cdeM"
                     /locus_tag="KPG09_001691"
     CDS             3905..4399
                     /gene="cdeM"
                     /locus_tag="KPG09_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBG0966308.1"
                     /translation="MENKKYANGGYSEDWYERGESTAKWFQNDREEYERAAYDEDRER
                     RGSNCGCSDSGGNRPRNCERFRREAEIREREAREAFCESSERKKEALAYECEARKLWE
                     EAEKYWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCN
                     CCRK"
     gene            4664..5929
                     /gene="hisD"
                     /locus_tag="KPG09_001692"
     CDS             4664..5929
                     /gene="hisD"
                     /locus_tag="KPG09_001692"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBG0966309.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6132..6884)
                     /locus_tag="KPG09_001693"
     CDS             complement(6132..6884)
                     /locus_tag="KPG09_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0966310.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANKKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(7002..7271)
                     /locus_tag="KPG09_001694"
     CDS             complement(7002..7271)
                     /locus_tag="KPG09_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966311.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            7746..8840
                     /locus_tag="KPG09_001695"
     CDS             7746..8840
                     /locus_tag="KPG09_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG0966312.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LRERSINGVISMCLKETDYQYSLSNKKGGY"
     gene            8842..9792
                     /locus_tag="KPG09_001696"
     CDS             8842..9792
                     /locus_tag="KPG09_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBG0966313.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGLKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINNNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKIDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            9785..10969
                     /locus_tag="KPG09_001697"
     CDS             9785..10969
                     /locus_tag="KPG09_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966314.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKKSLMREMNNVSKGVIDNEDTPSGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKSLLEISSYFKSKGLKIGIDTFSPFISYFTGQDLKKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIIAPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            10982..12478
                     /locus_tag="KPG09_001698"
     CDS             10982..12478
                     /locus_tag="KPG09_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG0966315.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNSHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHTVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSEGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            12501..13451
                     /locus_tag="KPG09_001699"
     CDS             12501..13451
                     /locus_tag="KPG09_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0966316.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLVSFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            13472..14434
                     /locus_tag="KPG09_001700"
     CDS             13472..14434
                     /locus_tag="KPG09_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG0966317.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            14806..15438
                     /locus_tag="KPG09_001701"
     CDS             14806..15438
                     /locus_tag="KPG09_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0966318.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            15849..15941
                     /gene="kdpF"
                     /locus_tag="KPG09_001702"
     CDS             15849..15941
                     /gene="kdpF"
                     /locus_tag="KPG09_001702"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBG0966319.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            15982..17664
                     /gene="kdpA"
                     /locus_tag="KPG09_001703"
     CDS             15982..17664
                     /gene="kdpA"
                     /locus_tag="KPG09_001703"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBG0966320.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            17689..19746
                     /gene="kdpB"
                     /locus_tag="KPG09_001704"
     CDS             17689..19746
                     /gene="kdpB"
                     /locus_tag="KPG09_001704"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBG0966321.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNVGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMIL
                     NLG"
     gene            19765..20391
                     /locus_tag="KPG09_001705"
     CDS             19765..20391
                     /locus_tag="KPG09_001705"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBG0966322.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNASYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNIEVAKILGLI"
     gene            20628..21536
                     /gene="cysK"
                     /locus_tag="KPG09_001706"
     CDS             20628..21536
                     /gene="cysK"
                     /locus_tag="KPG09_001706"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBG0966323.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            21563..22153
                     /gene="cysE"
                     /locus_tag="KPG09_001707"
     CDS             21563..22153
                     /gene="cysE"
                     /locus_tag="KPG09_001707"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBG0966324.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            22313..22501
                     /locus_tag="KPG09_001708"
     CDS             22313..22501
                     /locus_tag="KPG09_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG0966325.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(22555..23424)
                     /locus_tag="KPG09_001709"
     CDS             complement(22555..23424)
                     /locus_tag="KPG09_001709"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBG0966326.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDMNKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(23621..24865)
                     /locus_tag="KPG09_001710"
     CDS             complement(23621..24865)
                     /locus_tag="KPG09_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966327.1"
                     /translation="MENNLNIAKKNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYYMENNTNITIYENNTLQYKFDEESEDIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKSRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
BASE COUNT         9618 a         2678 c         4503 g         8205 t
ORIGIN      
        1 gttagaatat ggatatccat aataaatatg taaattttat aaaaaatata cctgtacctt
       61 ttttatattg tagaattgta aagagacaag aagatataga atatcgagta gaatatataa
      121 gtaagggtat gggcaaagta ttacaattag aagaaggtat ttgtgataaa aatatactag
      181 atgtattgcc tgtatttaag tctaaaaaat atttcaaaga gttgttctca aatgaagtgg
      241 attgtataaa aaggtacatt ccgacactta aaaactggat aaatataaaa aaacagataa
      301 taggggattc atacataatt ttgtattttg gtaaaattgt atttgattat agacagataa
      361 tcgattcctt tgataaaaag gaaaaagtag cttatattaa agacgaagaa ggcatatata
      421 tagattgcag tgaaaattta ataccaatat taaataacaa cattaaaaca acgaaagata
      481 tctttggtaa aaatgatata gaagtatggg gagagaatac aggaaaatta tttagagatg
      541 attatagagg aggagtatct agtaaaaaaa gatttttaca aaatcttttt gaatatgaag
      601 aaacattttt tatggttgaa aaatattttt tgtatgatga agacgaactt ttaggtacta
      661 tagggataat agataatata atatatagtg gttacagtaa tagaaattat aactcaaaag
      721 atttaatgaa gatgatagaa cactcaattc cagagaatat gttttataaa gatgtgtatg
      781 gaaattatat tggatttaat tcaggttttt tgaatttagc ttgtatgaat aaagaagaat
      841 tattagggaa gaatagttat aaaatatcag aagaagaagc tttaataaat aagatatttg
      901 aaagtgataa gggtgtagtt gaaaataaaa aagtagttac atttgagtta aatgtaagta
      961 taaatgatga aaataagtgt attgagatta caaaaaggcc gttttttgat agctatggaa
     1021 gtgttattgg aataattgga acagcaaggg atataagtag aagaaaaaga ttagaagagg
     1081 aaatggataa aactaggatg gaattttttg caaatttatc ccatgaactt agaacgccta
     1141 taaatttaat atcatcttca ttacaagtga tagaaaaaaa agaagcagat ttaatagaat
     1201 caaatgatac tttaaaaaga aatttaggta taataaaaca aaatggaaat agaatactga
     1261 ggttagtaaa taatgttata gactttacaa aaatgcaatc aggatattta gattttaaac
     1321 cagaagaatc tgatataatt gcatttatag aggagatatg tatgtctgta gctgattttg
     1381 caagccaaaa taatatacaa cttacatttg atacagaaat agaagaattt tcaatgttat
     1441 ttgattcaga aaaattggaa agaataatat taaatttact ttctaatgga attaagtaca
     1501 ataagaaaga tggaaaaata aatatatttt tatatgtgaa agataatgta tttaatatga
     1561 aaatatctga tagtggaata ggtataccaa aagaaaaaat agataaaata tttaatagat
     1621 ttgaacagat tgacaatgaa ctttcctaca gagttaaagg aagtggtatt ggactttcat
     1681 tagtgaaatc tttggttgaa ttgcatgagg gaagtatatc tttaaaaagt caacttggta
     1741 ttggaagtga atttatagtt tcattacctg ttagaagtga aaataatatt gaaaaatata
     1801 atcataaaag agaaatcagc aatgaactaa gtaaaaaatt agaaatagag ttttcggatt
     1861 tataagttta aatataaaaa ataaaatttt aaaaaagtat tgacaattga tttagaaggg
     1921 tttaaaataa attacaacat aataaataaa aaggcgatga ataggaagag taaatctaat
     1981 tcgattacca gagagaagag tccattggct ggaagacttt ttaaattaga gggattgaaa
     2041 actacctagg agctactttc tgaagtcatc tgcattagta gattttagta agaatagtcg
     2101 ttagctgcgt taaagtgtaa agtgagtttt catgatatat gaaaactaaa ttaggtggaa
     2161 ccgcgggaat aatactctcg tccttatgtt ataaggatga gagttttttg ttgtttaaaa
     2221 atataaacag taatcataat aattatagaa aatataaaag cgatgaatag gaagagtaaa
     2281 tttaattcga ctatcagaga ggaaagttca caggctgaaa gactttctaa gtttgagaaa
     2341 ttgaaaacta cctaggagct gtggtctgaa gttactttaa aaagttttag taagactaac
     2401 cgttaactgc gttaaagtgt aaagtggatt ttatcaataa atgataaaat caaattgggt
     2461 ggaaccgcga gtatagactc gtccctttat atagggatga gtctattttt attgcaataa
     2521 aagattgaaa ataaaaaaaa atcggaaaat tataaattta ggaggaaaat aaaatggaaa
     2581 ataaagtgaa aattggagta ctaggatatg gagttgtagg aagtggactt atagatataa
     2641 tagataataa taaagaaaaa agaagtatag aaatagttgg aattttagtt aataatttgg
     2701 aaaaacataa ggataaaaaa tattctaata taataactaa taatatagat gatatattta
     2761 ataaagatat agatatactt gttgaagtca tgggtggttt agaaccatca ttatcttata
     2821 taaaaaaagc attaaacaat aaaattcatg ttgttacagc aaataaagat ttgttagctg
     2881 aatgtggaga tgagcttgca aaattagcaa gtgaaaataa agtaagtatt aaatttgaag
     2941 cttcagtcgc aggaggtatt ccagtattaa aaccaataat agaatcacta gaaggaaata
     3001 atatagatag tataaatgca attttaaatg gaactactaa ttttatatta tctaaaatgt
     3061 atgatgagaa tctatcttat gatatggcat taagacaagc tcaagaatta ggttttgctg
     3121 aagcaaatcc agagtcagat gtattagggt atgatgctgc tagaaaacta tcaatattat
     3181 caactctagc ttatgacaat agagtatatt ggaaggatgt gtatttagaa ggaattacag
     3241 atatagatga aaaagatatt gaatatgcaa aaaaattaaa ttgtaaaata aaattgattg
     3301 gtcaaagtaa gtatgaaaat gataaagtaa gtgcttttgt tagacctgtt ttagtagaaa
     3361 aagataatat ccttgctaga atagacaatg agttcaatgc tgtcatagtg aatggagatt
     3421 cagtaggaga agtttctttt gtaggtaaag gagctggaag tttagcaaca ggaagtgcgg
     3481 tttattcaga tgttatagat ataatagata atagagtttc aagtatagat tcttttacta
     3541 aagataaaat acaagttaat aaaatagtta gagaaaagtg tggagcactt cttagattta
     3601 aaaaatgtaa taaagatgaa attctaaata tagttgagaa ttgtttagta aaattcgata
     3661 ttttaaatga tgatgatgaa cttgctatta tggtttatgc tgattctgaa tatgaaataa
     3721 acaacagttt atgtctaata aaagataaag gatattgcga aaaaatgaat aaaatgttga
     3781 aaataagtta aaaaagaaaa aataagttta tatgtaacat attttgacga ttttaatact
     3841 atatagtatg agatgaaaca aaacatctca taaaaaatta aaattcaagg gagagatatg
     3901 ttatatggaa aataaaaaat atgcaaatgg tggttattca gaagactggt atgaaagagg
     3961 agaatctaca gctaaatggt tccagaatga tagagaagaa tatgaaagag cagcatacga
     4021 tgaagataga gaaagaagag gctcaaactg tggatgttca gattcaggag gaaatagacc
     4081 tagaaactgt gaaagattta gaagagaagc tgagataaga gaaagagaag caagagaagc
     4141 attctgtgaa tcttcagaga gaaagaaaga agcactagca tatgaatgtg aagctagaaa
     4201 attatgggaa gaagcagaaa aatactggga tgaatattca aaatacaact ataaaggaat
     4261 cgaatattta gcagaagctg ctagattatt tgatgaaggt atggaatgtg aagctagaag
     4321 aaatggaaat aatggaggaa acaataataa ttgttgccat aaatgccata aatgtaattg
     4381 taactgctgt agaaaataaa aaaattcatt gacaaatcaa tagttaaaat atatcatatt
     4441 atgtataata aagagaaaat tcagaaaaaa ataatgatta gaataaattt aagtatattt
     4501 attagacaaa tagtgctcag ttagtgaggt gagtacaata tgattaaact cccatcctct
     4561 agcttttgat agagggtggg agtttttatt tattgatata tcttaaatga ggataatatt
     4621 gtaaaataaa tgagtatgag attgtaaaat taggaggtaa aaaatgaaaa gtattaaaac
     4681 atcaagtatg aaagaaaagg tagatgaaaa gatattaaga gagcaagtaa gttctataat
     4741 agaagatata agaaataata aggatgttgc acttaaaaaa tataatgaga agtttgatag
     4801 aaatacaaga gatgaattta gaattactaa ggaagaaata aaggaagctt ataagcatgt
     4861 agatgacgaa tttataaata acttaaagat tgctgccaaa aatataagag aatttgctaa
     4921 agctcaaaaa ctaagttttg aaaacccttt tgaaaaagaa atttatccag gtgtaatctt
     4981 aggtcaaact aatattccaa tagagtcatg tcttgcatat gttccaggag gtcagtatcc
     5041 attgttttca acagctctta tgcttataat acctgcaaaa gtagcaggag taaaaagaat
     5101 agtggcatgt tcaccaacta tgaaaaatac tgaaaagata aatcctaaaa cattggtagc
     5161 tatggatata gcaggtgctg atgaaatata tgcaactgga ggagttcaag ctatagcagc
     5221 atttacatat ggaacagaaa agattaatcc agttgacata attgtaggac caggaaataa
     5281 gtttgtaact gaagctaaaa gacaatgtta tggacaagta ggaattgatt ttgtcgctgg
     5341 gccaagtgaa gttcttataa tagcggatga aacttcaaat ccagtttata ttgcagctga
     5401 tttattagca caatgtgagc atgatttaaa tgcaagagga atattattga caaatagctt
     5461 ggaagtagca caaaaagttg aaaaaaatat agaaagtatg ctaaaagatt tgccaacaaa
     5521 ggacatagca tatagttcat gggaaaataa tggagagata atattggttg atgatatgga
     5581 agaagccatt aaaataagta atttttatgc accagaacat ttagaagttg cagtaaatga
     5641 gtataatgat atttgtgata gattaactaa ctatggttct ttatttattg gaaatctttc
     5701 tgctgaggtg tttggtgact atgtttcagg taccaatcat acattaccaa cacttaaagc
     5761 atcaagatat actggtggtg tttgggttgg aacttttatc aaaacttgta caaagcagat
     5821 atttaatgaa aaagctatac aatcattagc acctgtagct gaaaagctgg caaaagaaga
     5881 gggattatat gcacatgcaa aggctgctga ggttaggttt aaaaagtagt aaaaaatttt
     5941 attttggcag tcgagattta gtattagact gagataaaga aaacgctgag aacctgaata
     6001 aatactatat tttgtgggcc tcggcgtttc ttttatatct gtatataata ggataatact
     6061 acctgtgtgt ttaaatgcta acttaatatt tcttttctaa ataatgttta tgaaatagtt
     6121 aatattttta gttaatactt gaaaaaaact tttgctaact gtgcaaattc atctgcattt
     6181 gtcaatatag cagggtgtcc tccctgacta aaaatgtgaa tacttgcttt tgaaataaaa
     6241 gttgccattt gcttgtactc ttcttcaaga ttatgacgac acatttcgtc ttccttactt
     6301 cctgtaaata aaattggcat ttctaattta cacaattctt tatgaaataa agggcgtttc
     6361 ttgtttgcac attgcaatag agcttttgta tcaagattaa caacattctc ccaatctttt
     6421 ccatgacacc attcatagaa ttttcgtgct tgaatatctt gctttgattt ttcccttcca
     6481 gtgattagat tatctatgaa gttatcatta agagtacgtc catcaaagct atctgctata
     6541 attgcttcaa ctaaatcggg acgttctaat ccagtattaa cagctgccca tgctcctcca
     6601 cttgttccaa ttaagccaac tttaccacaa ttcaaatgtt caatcagtgc tattgtctgt
     6661 aaggcttcat catgccatat gtcaggagaa aattgagaaa ctctatcaga ctgtccatta
     6721 cctaaaaaat caattaagat acatctaaaa ttttctacat ataatggcac aagtgcttta
     6781 aacatattag aagaaccagt attaccatgc aggaaaatta tcggtttacc ttgtccatat
     6841 tctttataaa aacaagaatt accttgatac tgaaaatatg acatatattt tcctcctttt
     6901 tatttgttaa gttaattata acaatatagg tatgaatgaa aaacctcatg ttctttctta
     6961 gaataaaaat gtgccacgca taaaacgtga cacatttcta attataaatt ttgctgttta
     7021 agtattcttt gtatgctttt ctctgtcaaa aagtatttct ctgataactg cttgatactc
     7081 attccatttt ggaaatttat acagatttga gaattacgag aagctaagac ttgtttggtg
     7141 tttgtgtttt caccccaatg ctttttgtgc tcttgctttt tgggaatata aatcacttta
     7201 ccatctatat attcttgcac ttgctcaatc agttctttag gcagtaagtc aatcgaattt
     7261 tgatagctca ttttgcacct cctaaataat tactctttag gattagcaaa ggctactgct
     7321 actcacaatt aaaaataaaa ttttttatta tgtacaatag cctttgctat gcacaagttc
     7381 caattcccat aaactaaata ctcctttctg gtttgaaaaa ttcatctgaa cattgatttc
     7441 gattttaaga tagagttcaa tgacaaattt acatttttat tatatatatt cagatagata
     7501 attgcaactg tctttgtatg aggatatttt attatagtca atactataat atatatttat
     7561 atcatcaaac taatatgaac agttgaaagg aatatgaaca ataaaactta gtcaggaaga
     7621 ataattatga agcagacaat tgagaaattt gattttaaag ttttattgaa aatttattga
     7681 caaatttaac ttgtaaatat tatactaaac ttaataaata aagtttataa agtaaggtgt
     7741 tgtctatgaa aaatatagtt ggaaaaccgc aaacgataaa aaaagtaaat gaagatttaa
     7801 taaaaaacat tataaaaaat gaaggtccta taacaaaacc agaaatttca agcattactg
     7861 aattgagttt agctacagtt aataagattg tggagcaatt agctttgaaa aatgaagtta
     7921 aagtaagtgg actaagtgaa tctactggag gaagaagagc tcaattattt gaaataaatg
     7981 caaacttaga acacataata gctttatatt attatagaaa ttgttgtata ggggtagttg
     8041 ccaatttatt aggtgaaata atttatcaag aagaatttaa tataagaatg gatacatatg
     8101 aaaacgttaa ccaagatact ttttttgtta tagatactct aataaaaaag gaaaaaggaa
     8161 acaatataag ggctattgga attggtgtcc caggagttgt aaaaaaaggt gtagttaaaa
     8221 atataccaac tataaaaagt tgggaaggta taaatttaga aaatttaata aagagtaagt
     8281 acaatactaa agtgtttata gaaaatgata caaatattac tactattggg atttatcaaa
     8341 aacagtataa agacaaatat aaaaatatgg ctcttatgta tttagaagat ggtattggtt
     8401 caggtatagt tataaatggt gaattgtatt taggttctac aaattttgct ggagaattaa
     8461 gttatctaaa tatagaattt ggtaagtata actctaacaa cttaaatctt gaagattata
     8521 ttatgagttt aagaaaaaga tatttagaaa gtaaagataa taaatttaag aaagaaattt
     8581 tatctataat atcaaacttt ataaggactc ttgtatgtgt cctaaatcca caagctatga
     8641 ttatacagtg tagtatatta accaagagtg acatggagtt tatagaaaaa gatgtaagta
     8701 ataatgttgg agaagaaaat tttcctaaat taataaaagt agagtcattg agagaaagaa
     8761 gtataaatgg agtaattagt atgtgtttaa aagaaactga ctatcaatat tccttatcta
     8821 ataaaaaagg aggatattaa tgtgaaatat ttggtttcaa tagatggagg gggaacaaaa
     8881 actaaatttt gtgtaagtga cttagatgga aacatactta aagaacatac cactggttca
     8941 actaattata agtcagttgg aattaaaaaa acatatgaaa atattaataa tggacttaaa
     9001 aaaatattaa aagatttata tatcgattat gacgatattg agtacactgt atttggtata
     9061 tctggttgtg attctcctaa tgattataag attataatgg atgaaatatt aaaaattggg
     9121 attaacaaag aaaaaatata tctggcaaat gatgcagttt tagcttttta tgcacaagct
     9181 gattcccctg gtttagtaat tgtggctggg acaggttcaa ttatactggg aattaaagaa
     9241 gatggagaaa tatatagagt tggtggatgg gggtataatt ttagtgattt gggttctgga
     9301 tatgatatag ggagaaaatt attaaagaaa gtattgcttt attgtgatga atgtcatgag
     9361 tatagtgatt tatttagttg tgtcttggat ttctttggag caaatagttt tgaacaactt
     9421 gcatatatga ttactgatat aaacaataat gttgaaatag caaacctagc ttcattagtg
     9481 attgattgtg ccgaacaagg tgataaatta gctattgaaa tattaagaga atcatcaact
     9541 gaactatcta aattagctca agtaatattg agaaaaatat ctaatccaat gaaaatagat
     9601 aaaagcgaaa taaatatagt tttgtctggt ggtacattaa ataaaactgt atatgctcaa
     9661 atgttaatga ataatttaaa aacagaaaat acagataaaa atttaaaatt tatatctcaa
     9721 gaaaatcagc cagtatatgg ggggataaga cttgctatgg cattagctaa taggagagtt
     9781 aagcatggat agaaaaaagg taatagtaca aatttttact ggaggtttta aggctaaaga
     9841 aataaagttt aatgatataa aatgtaaact actagaatta ttggactgta ttgatataga
     9901 aaaggttatc atgggatgga gtgtagatga agaattatat gtagaaacta aaaaactatt
     9961 aaataaatat aatgttgaac tatatctttg gttgccatgt ttttctgaaa taggattact
    10021 tgaaaaaagt aatttactaa ttgattatat ggggaaggaa gtcaagggct atgctttaca
    10081 agaggatgaa aattttgaat tttattgccc aaataataag gaaaatataa aaaatataaa
    10141 gaaaatatat agtacttatt ttagtgatat taaatttgat ggaatttttt tagataaaat
    10201 aaggtatggt tctttttcga ataaactaaa tggtgttttt aattgttttt gtaaagattg
    10261 tttaaatata tatgaaaaaa ataatattga taaaaagtcg ttaatgagag aaatgaataa
    10321 tgtgtctaag ggagttatag ataatgaaga tactccatct ggtataacag aatacattaa
    10381 tggaaaatac aaatttaaaa ataaaatatg ggaagatttt tttgaaacaa aaaatcaaaa
    10441 tatatacaag tcattattag agataagtag ttattttaaa agtaagggat tgaaaatagg
    10501 tatagatact ttttctcctt ttatttctta ctttacaggt caagatttaa aaaaattaca
    10561 agaagtagca gattttataa aaccaatgat gtatagaact acaaaagctc cagctggttt
    10621 accatttgaa tttgaaaggt ttattgatga atccatacat aagaataaag agattggaaa
    10681 aagaaatttc ttagatattt taggaattaa agagtatgaa ggagatgtat atcctataga
    10741 atttgtacta gaggaaatcg attttatgac aaggaatctt aataaagata tatatgtagg
    10801 agttgaaata aataaaaagg aaattatagc tcctgttaca cctaagtaca taagtgaaaa
    10861 tatttcaaat ataaataaga caacagtaaa aggatatgtt ttatcttggg atttgttatc
    10921 agcaccaaaa gaaaatattg atgaaattat aaaatggttt cggaattagt gaggtgatat
    10981 agtgagtaag atattagaaa tgcaacaaat atataaaagt tttggaaagg tagatgtttt
    11041 aaaagggata aattttgagt tagaaaaggg agaagtaaga gctcttttgg gagctaatgg
    11101 agcaggaaaa tctacactta ttaaaatttt aggaggagtt cattctcaaa ctaaaggtaa
    11161 agtaattttg actggtaatg aaataatgtt taaaaattca catcattcta aagaaaatgg
    11221 tattagtata atttatcaag aattaagctt gataccaacg cttacagtac ttgaaaatat
    11281 ttttcttggt agagaaattt taaaaggaag tgtgttttta aacaagaaag acatgcttga
    11341 ggaatatatg aaaatttgtg aggattttga ttttaatata gacccacata cagtagtttc
    11401 taatttaagt gtagcaaacc aacaaatggt tgagataatg aaagctgttt catgtaattc
    11461 tgaaattatc ataatggatg aacctacaac atcacttact aataatgaaa aagatgggct
    11521 atttaaaata ataagtagac taaagtctaa aggtaaaaca atagtctata tttctcacat
    11581 attagaggaa gtttttaaag tatgtgatag tgcaagtata atgaaaaatg gaattatgat
    11641 tggaacttat gatgtagagg atttaactaa attaaagata actcaactta tgacaggaaa
    11701 tgataatcac aaacttattg agaagaagga ttatacattt gcaaagtatg aagagccacc
    11761 agtactagaa gtaaagaata taagctggtc taacaaaata aaaaatgttt catttaaagt
    11821 ttatagaggc gaagttgtag gtcttgctgg tcttgtaggt tcaagaagaa ctgaaattat
    11881 aaatttgata tttggtatag ataataaaga tagtggagac atttttatca atggtaaaaa
    11941 agtagacata acttctccta aggttgctat aaaaaacaaa ataggattga ttcctgaaga
    12001 tagaaaaaat ctaggattaa ttttagaaca agctatatac acaaactcat catcagtaca
    12061 aattgataaa ttcaagaaat ttttgtttat agatagaaat aaagaaattg agttttctaa
    12121 aaaaggtgtt aaggaattag gaataaaagt tagtgatgta aagcaagaag taaaagagct
    12181 tagtggtgga aatcaacaaa aagtagtagt ttctaagtgg ttagagaaag atttagattt
    12241 aataatatat gatgagccga caaagggaat agatattgct gcaaaagaag atattttcaa
    12301 tacaatcaaa gaattttcaa gtgaaggaat aggagtaata tttatatctt ctgatttaga
    12361 agaagtaata agagtaagtg atagaatttt agtaactaga gaaggttcta ttgtagatga
    12421 tttaaaaaat gagaatatac aggttcaaga tataatgaac agtatattta atgtataaaa
    12481 aatgaaaatg gaggactgag atgaagaaag cttttgagaa gttaaattta agagattatg
    12541 gagtattatt aggtttttta gctttatgta tagctataag tattgcaact ccatcatttt
    12601 taggtaaaca aaatatactt aatcttttac gacaatcttc tataattgga ataatttcag
    12661 caggtatgac ttttgttata atatctggaa actttgatat atcagttgga gcagtagcag
    12721 ctcttgcagg tgctataaca atgaaatttg cgactggtgg aactaatttg tttgcatcca
    12781 tgctcttagg tgtagcgatt tgtgcagtaa taggattaat caatggtata gtagttgcaa
    12841 agataaatgt accatcatta atagcaacta tggctatggt cactattgta agaggattgc
    12901 tgttgatgct tacaggagga tatccaatta ctgaaaatat accaatactt gattacattg
    12961 gaaatggata tttgtttaat ataccaatac cagtagtaat tttctttata gtggtgttag
    13021 tttcatttat tgtacttaat aaaactaagt ttggtagata tgtttattct gtaggaggga
    13081 atcaagaagc aagtaaactt aatggtataa atgtagattc tcacaaagtg aaagtgttta
    13141 ttattaatgc tgtacttgca ggaatagctg gaatcgtact aacaggaaga cttgggactg
    13201 caactgctat tgcaggtgaa ggatatgata tggatgcaat tgcatctgta gtaataggag
    13261 gtacatcagt ggcaggaggt tctggttctg tacttaaaac tgttatagga gtattactta
    13321 tgagtgttat aaacaacagt tttaatcttc ttggaataga tgtatatttc cagtacatat
    13381 ttaaaggatt aataatttta gcagcagttg gatttgattc ttatagcaag aaaaaacttg
    13441 cttcaagata aaaaataagg gggatttttt tatgaaaaaa ttaatgaaac atttagcatt
    13501 gttgttatca cttgttatga tatttggttt agttggttgt tcaaatggtg gtgacaaaga
    13561 aaaaaatgga gaaaagaaaa tagcagtatt actaccaggt tcaacaggtt actttgtagc
    13621 aactaaacaa ggtatagatg caaaatctaa agaattaggt gtaagcgtgg aatatgcaga
    13681 tgctcaatgg gatgctagta aacaattatc acaagctgaa gattttatgg ctaaaggggt
    13741 agacatgatt gcattatgtg gagttgattc tgctgtaagt gagagaatag ttaaagctgc
    13801 taatgattca gacgtcccta tagtagcatt tacaaattct ataggaagta acccaactgg
    13861 agaatttaaa ggtttagtta catatatagg acaaaatgag gaggaaacag gagcactaac
    13921 tggaaaaatt gctaaaaact tattaggaga aactggtggg aaagctgtat taatagaagg
    13981 agtaccagga actactccac aagtattaag aaaaaaaggt cttgaaaaag aattaaaaga
    14041 tagtaatata gaaatagttt ataatcaaac ttcaaattgg gagaaagagc aagctttaaa
    14101 agtaacagaa gatttaattc aaaagaaaac tgattttaat gttataatat gtcaagatga
    14161 taattctgct acaggtgcag gtcaagcttt aaaagatgct ggtcttaagg ataaagttaa
    14221 agtcataggt ttgggtggaa gtaaagatgg attaaaagct ataactgatg ggttaataga
    14281 tggtacaact tatatgtctg ctgtagaaga aggtggatta gtcatagaaa aggcatctaa
    14341 attccttaaa ggtgaaaaaa tagaacctgt tacacaaatt aaacaagttg aagttaacaa
    14401 agataatatt tcagagttca agggtgaatg gtaaattgtc atagataata aataagattt
    14461 ttagagttac tttagaaact atataaagta actctttttt taatttaatt tagtgataaa
    14521 ttattgaata gtatttctaa acaaataaaa aattaatata aacaaagact taattatact
    14581 aaattataag taaaactaat ataaacttat ggaaaaaagg aaaacgtcta aagtattgga
    14641 attactatag tgtaggtgtg gtattaaaaa aacataaaaa aacataaaaa aattgttgaa
    14701 aaaaatatag aaattttgta taattaaaga tttgaaaagt tcggtataca aagccaattc
    14761 ttttgtatat aatatatata aattcagaaa aaggaaggga tatgtatggc caggcggtta
    14821 tttttagaaa ggattggact tatattttta ggttcagcaa tattagcatt tggagtttat
    14881 aatttttatt atttaaacaa cataacagaa ggtggagtgt taggtatttt actactgtta
    14941 aaaaatttat tcaatataca accagcaata gcaaatgttg taatagatgg tttactactt
    15001 ttagtaggat ataaattttt cggtaaaaaa ttccttatat attcaatagt agcttctata
    15061 actttttcag tgttatatga cttatttgaa gctataggac ctctagtacc acaatcacaa
    15121 aatatgcttt tatcaactat tttggcaggt gtaactgttg gaactggtgt aggtatagta
    15181 gtaaaagcag gttgtgcatc aggaggagac gatgctttag cattagtaat ttctaagact
    15241 acatctttga acatagggca agtttattta gcaacagatg ttattgtatt gttgttatca
    15301 ttattttatc tatcagcgtt tgatatcttt tattctttga ttgctgttac aattagtggc
    15361 aaggtaattg attttattta ttatcatggt aaaagtttgg atatggatat aagtaatgat
    15421 atagttccag aatgttaata taaattaaat aactttagat tttattaggg gtggcagatt
    15481 tgccaccttt tttgtgtcta aatctttata gaaagtataa tcccatctag gtttttgcaa
    15541 atctcaaata tagaattatg ttggaatgac tgggaatacg attgctagat gtgcatttta
    15601 aaaacccaat tttatattta tgaaatacta atcattaatg gatgtttctt aatcctatct
    15661 ttatacctaa aatgataaga taaatgaagt agcaatacaa aactaaaatt tgtaaaagag
    15721 attaaattaa atggaggagc taaaatgttg gatatagtta tgatagcatt gttattcata
    15781 ggatttgttt ccatgaagtt acttgctgat tggtgtggga aacaaactga gaaaaaataa
    15841 tgtaatgtat ggagggatta cctatgtggt ttctggcagt tgtaattata atgttaataa
    15901 tatatttggt atatgcgctt attaatcctg aaaaatttta aacagtataa ggctaataag
    15961 taagttgaat tggggggaac aatttttatg gtgcagatag taatagtgtt agcgattttt
    16021 atgattttag taatacctat gggcaagtat ttatatcata tagctactaa tcaaaaaaca
    16081 tttggagata gactatttga taaagtggac aattttatat ataaggtatg tagtatagat
    16141 aagaaaaaag aaatgaactg gaaacagtat gcattagctt tattatttac aaatgcagtt
    16201 atggtattta taggatatat catacttaga actcaaagta tgcatatttt taatccaagt
    16261 ggaattaaaa gtatggaaca aggactatca tttaatacaa taataagttt tatgacaaat
    16321 acaaatcttc aacattattc aggtgaatca ggactttcat actttagtca gatgacagtg
    16381 ataatatata tgatgtttac atcagcagca acaggatatg cggctgctat ggcatttgta
    16441 agaggtctag tcggtaaaaa gaaaacattg ggaaattttt atgtagattt aattaggata
    16501 acaacaagag tacttttacc aggagcttta attattgggc ttatattagt tacacaagga
    16561 gtaccacaaa cttttgctgg gacagaaaca gtgactacta tagaaggaaa gcttcaagat
    16621 atagctagag gtcctgtggc agctcttgaa tcaataaaac atttaggaac aaatggtggt
    16681 ggattctttg gttcaaattc atcacaccca tttgaaaatc caacaataat atcaaatata
    16741 gtagaaatat tatctatgat gatattacca ggagcttgtg ttgttgcatt cggtcacatg
    16801 ataaagaata aaaaacaagg ttgggttgtg tttggcgcaa tgtcaatcat atttttaata
    16861 ggtctagtag tgtgctttaa agcagaaagt gcaggaaacc ctattttatc acaattaggg
    16921 cttaatcaaa gtatgggaag tatggaaggt aaggaagtta gatttggtat agcacaatca
    16981 tcattattca caacagtgac tacttccttt acgacaggta cagttaataa tatgcatgat
    17041 acactgactc ctttaggagg attagtacca cttttaaata tgatgctaaa tgtagtattt
    17101 ggtggtaaag gcgtaggtct gatgaatatg ttgatgtacg ctataatagc agtattctta
    17161 tgtggattaa tggtaggaag aacaccagag tttttaacta agaaaatcga aggaaaagaa
    17221 atgaagctta ttgctttact tattatatta catccacttt taatactaat gttctctggt
    17281 ctttcagtag caataccagc agggcttgaa ggaatttcaa atcctggatt ccatggatta
    17341 tcacaggtat tgtatgagtt tgcatcttca gcagccaata atggttcagg atttgaaggt
    17401 ctaggagata acactatgtt ttggaatata acaacaggta tagttatgtt ctttggaaga
    17461 tatgtgtcta ttatagtact actagcaata tcaagtttgt tagcatctaa aaaagctgta
    17521 aatgaaagta taggaacact tagaacggat aattttacat ttacaatagt tttagtactt
    17581 gttgttttaa ttgttggtgc tttaacattt ttcccagcct tagcgcttgg acctatttcg
    17641 gagcatctag tgttgtggca ctaaaaaatt gggagggagt aaagttttat ggaaactaaa
    17701 aaaacaaagt ttattacaaa agatattttc aaaacttcca ttatagaagc ttttaaaaaa
    17761 ttaaatccta aatatatgat gaaaaatcca gttatgtttg ttgtagaaat tggatttata
    17821 gtaacattga tacttacaat agtacctagt ttgtttggtg atgaaggcaa taatttaaga
    17881 gcttataatg ggattgtaac aataatctta tttataacag tcctttttgc aaattttgct
    17941 gaagctgtag ctgagggaag aggtaaagct caagcagata cacttaagaa aactagaaaa
    18001 gatacagtag caagggttat agatacgtta ggaaatgaaa aaatgataag tgcaagtgag
    18061 ctaaaacttg gtgatattgt tttagtaaat gttggagaag ttatcccaaa tgatggagaa
    18121 gttatagagg gcatagcttc tgtggatgag tcagcaataa ctggagaatc tgcacctgtc
    18181 atgagagaat caggtggaga tttcgcatct gttacaggtg gtactacagt tgtaagtgac
    18241 tggttaaaga taagaataac aacaaaacca ggagaatcgt tcttagataa aatgatagct
    18301 cttgtagaag gtgcttcaag acaaaaaacg ccaaatgaga ttgctttaaa tactttgtta
    18361 gtagggttaa cacttatatt tatggttgtt ttaatatctc tttatccaat ggcaaactat
    18421 gcaggtgtaa agattccaat ttctactatg atagctcttt tggtatgttt aataccaact
    18481 actataggtg gattgttgtc agctattggt attgcaggta tggatagagt aactagattt
    18541 aatgtaattg caatgtcagg aaaagcagta gaagcttgtg gagacgttga tactatgatt
    18601 ttggataaga caggtacgat tacttatggg aatagacttg catcagaatt tatagcagtt
    18661 ggaaatgcag attcaaaaga tttaataaat tattctgtta tgtgttcatt aaaagatgat
    18721 acaccagaag gaaaatctat agttgattta ggtatgaaat taggttcaac aggtaagaca
    18781 aaagaggcag aggaagctga gtttgtagaa ttttctgcac aaactagaat gagtggtatt
    18841 aacttagctg atggtagagc tataagaaaa ggtgcttatg atagcataat aaaaagagta
    18901 aaagaatctg gtggtacagt gccagaagat ttagaagaaa atgtaaatcg agtagctaaa
    18961 ctaggaggaa cacctttagt agtgtgtgta gataatgaga tatatggaat tatctactta
    19021 aaagatacag taaaatcagg gcttgtagaa agatttgcta gacttcgaga gataggaata
    19081 aagacaataa tgtgtacagg ggataatccc ttgacagcag ctacaattgc aagagaagct
    19141 ggagtagatg gatttattgc agaatgtaaa ccagaagata aaatagatgc tataaaaaaa
    19201 gaacaattag agggtaaaat agttgcaatg acaggtgatg gaacaaatga tgctcctgca
    19261 cttgctcagg cagatgtagg tcttgcaatg aatagtggta caacatctgc aaaagaagca
    19321 gcaaatatgg tggatttaga ttcagaccca actaagatat tggaagttgt tgaaatagga
    19381 aaacagcttt taataactcg tggggctttg acaacattta gtatagcaaa tgatgttgca
    19441 aaatattttg cgattatacc ggctatattt actcttgcaa tacctcaaat ggagatatta
    19501 aatgtaatga aactttctac atcatttagt gcgatattat cagctttaat atttaatgct
    19561 ataattatcc catgtttaat acctcttgca atgaagggag taaaatatag acctatgaaa
    19621 tcacaagcta tgttaatgag aaatatgctt atatttggtc ttggtggagt tatagtacca
    19681 tttttaggaa taaaattaat tgatttattg attacacctt tagtaatgat tcttaattta
    19741 ggttaataaa tggaggatat aaaaatgaaa acattaaagc cagcattact tgtaagtatt
    19801 gtattgttag ttgtttgtgg acttgtatat ccacttgttt tgacaggtgt aagccaagtt
    19861 gcatttaaag ataaagccaa tggaagtatg atagaagtaa atggtgtaaa agtaggttca
    19921 gaacttattg gtcaaagctt tacagatgcg agattcttta agggaagagt atcatcagta
    19981 aactataata cttatacaaa agaagattta gtaccagata aagatggaaa tgcatcttat
    20041 ggaggtgtat catcagggtc ttttaattat ggagctacaa atccagaact tcatgataga
    20101 gtacaaaaag atatagaaaa gtttttaaag gataatccaa ctgtcaaaag agaggatata
    20161 ccaacagatt tacttacagc atcaggttca ggacttgacc ctaatataag tccagcctct
    20221 gcaaaaattc agataccagc tatagcaaaa gcgtctggaa tatcagaaag taaattacaa
    20281 aaaattgtag atgataatac ttccaaaaaa ctttttggag ttttaggtga agatagagtt
    20341 aatgtactaa aggttaatat agaggtcgca aaaatattag gtcttatata gtattttgtg
    20401 gaattttagt aacaaaatat tactcaagat ttaatttaaa ggttaaattt taaagagtga
    20461 aaatcaataa tgattttcac ttttttattt gaatattact aatatttgga ataaagtgat
    20521 tgtaagtata aaatccaaaa aaaggttgca ttatcaaaat gataatacta taatatattt
    20581 atcgttatca atttgataat agatttggac atataggagg aaatagtatg ttatataata
    20641 acgcattaga gttgatagga aacacacctg tagtaaggct gaataattta ggctgtacta
    20701 atgggtgttc taacatatat gtaaaattag aaaaagtaaa tccagcagga agtataaaag
    20761 atagagctgt ttatgaaatg ttagagggct tagagcaaag aggagaatta aaaaagggag
    20821 atgtactagt tgaagcaact agtggaaata ctggtattgc attatctatg ataggaaaat
    20881 taaaaggata tgaagtagta atcgtaatgc ccgaaacaat gagcgttgag agaagagatt
    20941 taatgaaggc ttatggagca aatttggtgc ttacagatgg taaagctgga atggcaggtt
    21001 ccatagaaaa agcaaatgaa cttttaaaag aaaatccaaa ctataaaagc ttaaaacaat
    21061 ttgaaaataa agataatcca aatgcacatt ataaaacaac agcagttgaa attatgaatg
    21121 atgtcaaaga tttagatata ttcgtatgtg gagttggaac aggtggtact ttaataggaa
    21181 ctgcaagata tttaaaagaa caaaatccaa acataagagt aattgcactt gaaccaaaaa
    21241 aatctccagc tatatcagaa aacaagactg gacctcataa aatacaagga ataggtgcta
    21301 actttgttcc tgaaaattat gattctagtg tagttgatga agtaatatta gtagatgatg
    21361 aagatgcatt tgatacagtt aaattacttg cagcaaaaga gggaatttta gtagggatat
    21421 cttctggagc aaatatattt ggtgctatag agatggcaaa aaagtatcca gataaaaaga
    21481 tagttacagt agctccagat ggagttgata agtatatgtc tatgggaata ttttaataat
    21541 tttatttgag ggagtgaata ctttgtttaa gaaaataaat aaagatattg aatatataat
    21601 gaagaatgat ccagcagcta ggtctaagat agaagttttt ttactttatc catcagttca
    21661 tgcaatgata atgcatagga tggctcatgc tctttataag aagaagaagc tttttacagc
    21721 aagattaata tcccagatat ccagatttat gactggcata gaaattcatc ctggtgctaa
    21781 aatgggggaa ggtatattga tagaccatgg aatgggtgtt gtaattggag agacagctga
    21841 agtaggtaat agggtgacaa tatatcaagg agctacttta ggagctacag gaaaagatac
    21901 tggaaagaga catccaactg ttggtgatga tgtattaatt ggtgctggaa caaaaatact
    21961 aggaccttta aatataggtt caaattcaaa aattggtgca aattctgttg tcgttaagga
    22021 tgtacctaat ggtgcaacag ttgttggtat accagcaaaa atagtaaaaa taagaaacct
    22081 agaacctgta aaaaagaata aaaaagaagt gtcctatgag tatgatgaat tagataatgt
    22141 atattatata taggatttat ataactataa aataaacttt ataaaaatta ttttaaaagg
    22201 tatttttttc aaatttgagg acataataaa aaagaactga atgttacagt tttggttcat
    22261 caatctttga taaacaagat tttaaatttg tagcgtctag gaggatttta ttatgaaagc
    22321 aaatgttaat caagatactt gtataggttg tggattatgt ccatcaatat gtccagaagt
    22381 atttgatatg aaggatgatg ggaaatcaca tgttatagtt gatgaagtac caagtgatgc
    22441 tgaagaatca gcagcagaag ctagagaaag ctgtccagtt gatgcaatag atgtgcatta
    22501 ataaaataaa tgcctataaa aattgaattt tcaattttta taggcatttg tcttttattc
    22561 agttagtatt tcaacccctg tttctgtaac taaaaccata cattcccatt gagcagataa
    22621 tttaccatct tttgtataaa tagtccaatt attttcttca tcaacaaaaa tttcgtgagt
    22681 tccttcattt accataggtt ctattgtgaa tatcatccca ggaaccaata gcatctcagt
    22741 ccctttttta gatacataac ttacaaaagg gtcttcatga aagcttaatc caactccatg
    22801 accacctatt tctctaacaa cagagtatcc atttttcttt gcataagagt tgatagcatc
    22861 acctatatct cctaaaaatc cccatggttt ggcaacttca agaccttttt ggacacattc
    22921 ctttgtaact tgaactaatt tttttgcatt ttcattaaca ttacctatca taaacattct
    22981 tgaagcatct gaatagtatc cattaaaaat agtagaaaca tctacattta taatatctcc
    23041 atcttttaag ataatgtcct tgcttggaat accatgacaa acttcattat ttactgatgt
    23101 acatacactt ttaggaaatc cattatagtc tagtggagca gggatagctc cattttgtag
    23161 tgtaaagtca tgaaccaact tattcatatc ttctgtagac attccaattt cgattttatc
    23221 tgaaatataa tccaaaattt ttgtgtttat tacagcactt ttttttattt cctcaatttg
    23281 actttcattt tttataatat catgagttgg tacaatatga cctaacttag caaatgaatc
    23341 tattttatta tccatatcca tatgacattt tttatatttt ttagtacttc cacaccaaca
    23401 gctatcattt ctatttaact tcataacttt tcatccttct tttattattt atttaaatta
    23461 tgggttacat aaactatttt aaaacataac cattataaat attataacat aaatactaag
    23521 aaatccttta tagatagtga tttattcaaa gtataaatta ttatatggtt attgttgatg
    23581 aaaagaggat agttaaatgt attattggat attagcgcac ttaatagttt agttcaagtt
    23641 tttcaagaaa atcatatgtt agaggacaat tttcctttaa agtatttttt gaatcttcat
    23701 caagataata atgcacaaat gattctgcaa aaaattcttc tataaacttg cttatatatg
    23761 catgtgcaac ttgtttaggg tcataaagct tatatctttc ctgagcaaat acttccataa
    23821 attcttcaga atttgatttt ttatttaaaa catttttatc tatagcatgt gcagtttcat
    23881 gtaattctaa attttttgat gaatgtacat ttgcatagct agggttacta taacctattt
    23941 tagctactat agaattattt ctatatatac caggtacatc tttccaagtc ttgcctgatc
    24001 ttacccaaca tggaggtaat tgatatttta aatcactaaa ttctggttca tcagttagat
    24061 ttgaatttat taattttatt tttatatcct tttctttaag tgtctgtaaa tatttaatat
    24121 ttatttttgc aagtctgctt ttcatatttt caacttcatc tttatcataa ttcaagtcat
    24181 caggaagtat aagcatttgt tcaattatat ctatgctact gtcttttata tcttcagatt
    24241 cttcatcaaa tttatattga agagtattat tttcatagat agttatattt gtattatttt
    24301 ccatataata agagtgaata tcctctaagt ctaaaatatt catttgttgt aaaatatgtt
    24361 ttgtttcgcc actccatact actaaagaaa gtgaattaaa attaattagt ggattttcct
    24421 ttgcttttat atcattgctt atatctacat caaatacata ttcaattgag tcttctatgt
    24481 taagactatc tgtattcttt ttaatatata aatatgcttg ttttaagtct atataaatct
    24541 catctaaaga ttcatcataa aattctgata aattattagt aaagagttct tcacttaaaa
    24601 gttcattttt atcagttata ctttcaatta aaatattaat ttcttttatc atatcgtaaa
    24661 tacttatttt tctatttgct ttttcactag atacattgca tggcgcaaga gcaatgcaat
    24721 aaaaatcttt ttctttttta taaaaggctt taagtgattt tgataaaagt gtaaagtcta
    24781 cacctatctg taagttgacc ttaagtgagt cattttgtaa taattcagta ttattttttt
    24841 tagctatatt taaattgttt tccataatca ttgccccctt tttattaggg tatcataatt
    24901 tttttatatg tgtcaatttg tttttttgaa aattataatt tttttgaaaa ttttattttt
    24961 tataatttgt gaatttttta ttatagaaat aaatatgtta ttat
//
LOCUS       DAELSY010000048        24795 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.048, whole genome shotgun sequence.
ACCESSION   DAELSY010000048 DAELSY010000000
VERSION     DAELSY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24795
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.048"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            107..784
                     /locus_tag="KPG09_001711"
     CDS             107..784
                     /locus_tag="KPG09_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0966328.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            760..1221
                     /locus_tag="KPG09_001712"
     CDS             760..1221
                     /locus_tag="KPG09_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0966329.1"
                     /translation="MGGAGDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            1289..2068
                     /locus_tag="KPG09_001713"
     CDS             1289..2068
                     /locus_tag="KPG09_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG0966330.1"
                     /translation="MNSKIYYFSGTGNSLVVSKILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIIKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPSDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(2318..2944)
                     /locus_tag="KPG09_001714"
     CDS             complement(2318..2944)
                     /locus_tag="KPG09_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HBG0966331.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDNRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIEVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(3087..3344)
                     /locus_tag="KPG09_001715"
     CDS             complement(3087..3344)
                     /locus_tag="KPG09_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966332.1"
                     /translation="MILNRTELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FSNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            3670..4182
                     /locus_tag="KPG09_001716"
     CDS             3670..4182
                     /locus_tag="KPG09_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966333.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEETYSGCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDISNYYLVANYI
                     RCLKKYKLID"
     gene            4437..5141
                     /locus_tag="KPG09_001717"
     CDS             4437..5141
                     /locus_tag="KPG09_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG0966334.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTDGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            5138..5908
                     /locus_tag="KPG09_001718"
     CDS             5138..5908
                     /locus_tag="KPG09_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG0966335.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQSLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            5905..6681
                     /locus_tag="KPG09_001719"
     CDS             5905..6681
                     /locus_tag="KPG09_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG0966336.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            6736..7428
                     /locus_tag="KPG09_001720"
     CDS             6736..7428
                     /locus_tag="KPG09_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0966337.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            7447..8871
                     /locus_tag="KPG09_001721"
     CDS             7447..8871
                     /locus_tag="KPG09_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0966338.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMPYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANSRGR
                     FVFAVIIMALFSPFIYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMKGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(9111..11579)
                     /locus_tag="KPG09_001722"
     CDS             complement(9111..11579)
                     /locus_tag="KPG09_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0966339.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSFISALPSAIYASRVSPKIAMAGSTTKIKRKIKREKAIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIDKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYNSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNISLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(11582..12250)
                     /locus_tag="KPG09_001723"
     CDS             complement(11582..12250)
                     /locus_tag="KPG09_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0966340.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLKINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            complement(12536..13063)
                     /locus_tag="KPG09_001724"
     CDS             complement(12536..13063)
                     /locus_tag="KPG09_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBG0966341.1"
                     /translation="MKSFANNITFSRIVFALLMLSSTFCSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            complement(13280..14440)
                     /locus_tag="KPG09_001725"
     CDS             complement(13280..14440)
                     /locus_tag="KPG09_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0966342.1"
                     /translation="MDKISNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNINMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNPHFGMGLHISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            complement(14428..15102)
                     /locus_tag="KPG09_001726"
     CDS             complement(14428..15102)
                     /locus_tag="KPG09_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0966343.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDSLKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            complement(15923..16750)
                     /locus_tag="KPG09_001727"
     CDS             complement(15923..16750)
                     /locus_tag="KPG09_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG0966344.1"
                     /translation="MNFYKIAVCQMMTTENKIENINHAVDMVTEAAINGAKIIVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEYGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGVEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            17132..17452
                     /gene="sugE"
                     /locus_tag="KPG09_001728"
     CDS             17132..17452
                     /gene="sugE"
                     /locus_tag="KPG09_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBG0966345.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            complement(17585..18265)
                     /locus_tag="KPG09_001729"
     CDS             complement(17585..18265)
                     /locus_tag="KPG09_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG0966346.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            18661..19713
                     /locus_tag="KPG09_001730"
     CDS             18661..19713
                     /locus_tag="KPG09_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG0966347.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGTIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPMTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            19760..20176
                     /locus_tag="KPG09_001731"
     CDS             19760..20176
                     /locus_tag="KPG09_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0966348.1"
                     /translation="MIHMTGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRRLEDRIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            20205..20678
                     /locus_tag="KPG09_001732"
     CDS             20205..20678
                     /locus_tag="KPG09_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0966349.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDEHLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            20725..21483
                     /locus_tag="KPG09_001733"
     CDS             20725..21483
                     /locus_tag="KPG09_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0966350.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNENVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            21507..22346
                     /locus_tag="KPG09_001734"
     CDS             21507..22346
                     /locus_tag="KPG09_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0966351.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            22797..23444
                     /locus_tag="KPG09_001735"
     CDS             22797..23444
                     /locus_tag="KPG09_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0966352.1"
                     /translation="MNILIADDEISMLKILCAYFKKENFNVFTAKNGEEALDIFYENK
                     IDLAILDWMMPIIDGIEVCKEIKENSNTRVLMLTAKSQSEDEIEALNIGADEYIKKPF
                     DPRVLIIRAKKLINYKNTINIKDLKIDFGSSKVFKGDKELLLTKKELELIRCLINNKG
                     MVLSREKLLDLVWGLDYEGDFRTVDTHIRRLRVKIGEDIIKTYRGLGYSLEDFND"
     gene            23437..>24795
                     /locus_tag="KPG09_001736"
     CDS             23437..>24795
                     /locus_tag="KPG09_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0966353.1"
                     /translation="MTKLNKKLSISISIVVVVVYIISIIINSMFIHRYYLHEKRNILD
                     NVENEIKDKDIDKLKSDITLIEEKNNIDIVYIELDKNHKDKGNIDKINQDLLNAFWKK
                     GLSLNKFWIEENSLNNIDKKSINKIYNQGKTKYSLLVKFIKKDNYLFAISIPIEHSEE
                     TIGIVNRFNIIMGIFSVVIITILTFILSNRVIKPMERLKFLSKDISELNFRTEDIKTN
                     DEIEELAYSINIMSEKLEKAHNELNKRNENLKTFISDASHEMKTPIALIKAYAIGMKD
                     GLDDGTYTDTIIEQAENMTNTINSLLYWAKYEKKERSLCLVDLKERLYKSLKNYELLI
                     ERGSISVKCSVDDKELIISSDEDSIDMVFNNLISNAIKYTNNNEIEINLFEENERIIL
                     SIKNGIDYNMGDDIENMWKPFYVLEKSRSKELSGTGLGLTIVRTILEENNFRYRVEIC
                     DGI"
BASE COUNT         8993 a         2985 c         4145 g         8672 t
ORIGIN      
        1 atttaagctg tgtaagtata gctttaaata aatggtttat attgacagct aaaaatatag
       61 ttgatatact tattgttgat ataacaacta aaaaggagat atagaaatga taattacagt
      121 tataaatgga agccctagaa aaaatggagc tacatcaaaa gttttaactt acttgtataa
      181 agatatagaa agattaatac cagatgtaaa aattaattat tttgatttaa gtgaggtaaa
      241 tccatcatat tgtattggtt gtttaaattg ttataaaatg ggcaagtgta tcaatcaaaa
      301 tgataaagta gagtatattc atgatataat tactaagagt gatggtgtta tatttggtag
      361 cccaacttat ggaagtagtg taactggact tttcaaggtg tttacagata gagcacatat
      421 gatgcttgaa agattattgt atcgtaagcc atgtatagca gttactacat atgaaaatgc
      481 acgtggttca aaagcaattt cttttataaa atccatggtc ttagattcgg gaggttatgt
      541 ttgtggtagt ttatctataa aaactggatt taatcaaaat cctataactg aaaaagtaga
      601 atcaaagata caaaaggtat ctaaaaaatt tatttattgc atagaggaaa agaaaaatcc
      661 acctgtgctt tctcaaatct ataattttat tgctataaat gctgttttaa agccaatggc
      721 ttttaaagat attgagcaat ataagggaat aattgataga tgggaggagc aggggattat
      781 ttaaatggag gtaaaatgaa agattttgag aaactatatg aagctacaac taagttagta
      841 agtacttcgt taaaagtaaa agatgattta aaaaagatgt ttaaacaaaa tggatatgat
      901 ataacaactg accattatgc gttattaagg tttctatggg agcaagatgg aatttctcag
      961 atagatttat gtgaaaaatc atgtaaggat aaatctaata caacaagaat tttagacgtt
     1021 atgaaaaata agggattaat tgtccgtaaa gtagatgtta aagatagacg taagtttcaa
     1081 atattcctaa cagatttagg tagagaatta gaagaaccat taaatgaaat tgcaagtata
     1141 tatgcaacag agacatttaa atctatctct gatgaagaat tgcctttgtt tactgatatt
     1201 ttggatagaa ttggaaagta aatataaatc ctattttaag agacttttta actattgatg
     1261 atgtagaaat atagaaagag ggataagaat gaatagtaag atttattatt tttctggaac
     1321 aggaaattcg ctagtagttt caaagatttt aaaagaaacg ttaaatggaa gtaaagaagt
     1381 tataccttta gctatacata gaaaagaaaa taatatagac ataaatgaag atatattggt
     1441 aattgttttt cctgtttatt ttgctgatgt tcctgatata ataaagtctt ttgtagagaa
     1501 attaaatttt aatattaata ctcatatata tgccattgcc acttgtaatg gggtagctgg
     1561 acatagttta tttactatag ataagttact taaaaaaagg gggaaaaaat tatctgctgg
     1621 ttttttaatc aatatgcctg gaaatgcttt gataacacct tcagatgttg aagaagaaag
     1681 attgaaaaat cagactaata gggtagctga gattgcaaaa tgtatcaata acttggagat
     1741 tggaaatata gaaggaaaaa acaatttaaa gtcacatata gatagtgttg tacttcgttt
     1801 agtaggtaag aatatgcaaa ttgcaactac taaatttttt gcagaaaata aatgtaatgg
     1861 atgtgggaca tgtaaaaggg tttgtcctgt agagaatata agtatagttg ataaaaagcc
     1921 aaagtggggt aatgagtgtg aaagatgcct agcttgtttt cactggtgtc caaaagaggc
     1981 tattaatgta aaaaaatctt ttttaacgaa taggaagaag tatcaccatc cagatattac
     2041 tgtaaaagat atatttttag ataaatagat atatttgact tgtattaata attgttttat
     2101 taaataaaaa cgttagagat ataaaagaaa tataaagtaa tcttgtaaag agtaatgtta
     2161 cctagactaa atggaataga gattaaaaag aacaatataa ataataaatt atgttaatag
     2221 tatatagtta cactaatata aaataaaagt gcctcataaa tagaagtttt tgaggcactt
     2281 attatttggc taaattagtt tttattattt tgtaatatca accgtttata atctcgaaca
     2341 ttttttttat atcttctgat gccatttcaa attttgattt ttcaatcagt atgtttccac
     2401 cagtcataat atatgatgga aatactctta aattatcata ataaagttca ttattacgaa
     2461 gtttatatgt tgaaatggaa gggatgacat tcttttttat atgcttttta gtaatcatat
     2521 taaaagattt ttttgcaaca tctcccatta acattactac ctctaggttt ttaaataatt
     2581 taatttcttc ttctaaaata ggcatatgta atttaattgt atcacgagtt attgtatatt
     2641 ctgatttagg aagttttact gcattagtta tatagatacc catatctagg atatcattta
     2701 tgttagatac ttctattcct gcactgtcaa ataaaggaat agtagttttt aaataatcag
     2761 catctttatc tacactataa aaatcgtcat tggtgttagt tgggacaacc tcattaatca
     2821 ttatagtttt tattttgtct gtttctattg ttatattagg aaggtaggat attattctgt
     2881 tgtcatcaaa aagattgttc tgtttaataa attcacttaa ctttgatttt atatttattt
     2941 tcatataacg agtctcctta gttttaagct ttaaattact ataaaaagct agttttaaag
     3001 cacacaattt tgaaaatata gtagcatatt tgaaagtata ataacatata aaacatgaca
     3061 acagaatgtc atgttttata aataatctat cgcaacttag aatttattag agactctgcc
     3121 tgttcataat cgtctttatg aacataaatt aagtactcat aagagaaatc ttccttttga
     3181 cctaaattgc caagtgttat ttcattagaa aaaggttttt tagagtaagc attatttttc
     3241 actttatact tatatttaat tttatttact gataatatat tgcatatctc gttaaatcta
     3301 tttaaagaat atcctcggaa aagctcagtt ctatttaaaa tcataaggaa aacctccttt
     3361 taaaagtaat atattgaaaa gcataggaga aatgtactat aagttgttat aaataaaagg
     3421 tagcaaattt aaattgattt gtaaagtttt taagagtatt atattagtga taaaataaat
     3481 aatttttata atctaactaa taacaattta aaataggtaa cttatgtaag aatcgtaggg
     3541 actacggaga tatcctgtag agaattaata agacatattt aagtatgcaa aattctatta
     3601 agtaggaacc ttgtaacttt agtcatgtga gatttataag gatatttaag tattgaaagg
     3661 agagtttaat tgaaaaaaat atctcaagaa aaaataagta cacatatcca agaagtacca
     3721 caggaaaaaa ttgaacagtg gaaatcttta tatgaaaaat ataaaagtat tttaaaagta
     3781 aattctaagt ctgttttaga aataatagag tttttaaagc gaaaatatgt aatagtagaa
     3841 gaaacttcag aggaatttaa acatgtagtt atcaataata ttaagttaaa caagcctttt
     3901 tcacaaaaga tttccagtga taaagaatta aaacctattg tatttacaat tccaaatgaa
     3961 ggaaatgcaa aaaaattgta cgatactcaa gaggaaactt atagtggctg taagattatt
     4021 gtaggtatgg aatttgaaac aggttgtgtt tttgtagaag gtagtgatga gcttaatgat
     4081 gaaataactt cttttaaagg attagataaa tatgatataa gtaattatta tttagtagca
     4141 aactatatta gatgtctaaa gaaatataaa ttaatagatt agtgtttgat taataaatta
     4201 gtgcttgatt taaggattat agagtaaatt tgaagttagt gtgataatga aactaagctt
     4261 aaatctaata taactgtaaa acataaactt aaatagagtt gccaaaataa aattggtaac
     4321 tctatttttt attttaattt ttattaaaat tagtaggtgt tatagaattg ttattttttc
     4381 atgttagtat tctcttagta atagatttaa ggtattacaa gaagggagaa attaatatga
     4441 aagatttaat tcttgaaaca aaaaacttat caaaaagata tggagaacaa atggcagtta
     4501 acaatgtttc tctacaaatt gaacgaaata gtatatatgg acttttagga ccaaatggag
     4561 caggaaagtc cacaacactt aaaatgcttg ttggtcttct tcgcccaaca gatggtcgta
     4621 ttatttttga tagaaagcct tggaaacgag aatctcttgc aaaaataggt tctttaatag
     4681 aatcccctgc attgtatgga aaccttacag cagaggaaaa tttgttagta catacaaaaa
     4741 tattgggaat acctaaagat aaaatttatg aagtgttgga aatagtaaat ttgaaaaata
     4801 caggtaaaaa gagagcttca caattttcta tgggtatgaa acaaaggtta ggaatagcta
     4861 ttgcactttt gagtgaccca gagttgttaa tactagatga accaaccaat ggtcttgacc
     4921 cctttggcat tcaagaattg cgtgaattga ttgcttcttt tccaaaaaaa ggtataactg
     4981 taattctttc tagccatatt ctatcagagg ttgctcaagt tgtagataat ataggaatta
     5041 taaatggagg acgactacta tttcaaggaa tgcctgaccc taatgaaaat ctagaagaat
     5101 tttttacaga tgttattctt aaaggaggac aaaacagatg aatgctttac aatcagaact
     5161 tttaaagtat aaaagaacat ttatgggtaa acttattgtt tttttcccag tgtcttttgc
     5221 agcatatgca tttataatgc aatcaacact catgcaaaat ccattatctc aaacaacttc
     5281 ttgggcgtgg caaagtcttt tagcattaat ttttaactgg tggtcatttt tatttctgcc
     5341 tattggattt gcattgtttg ctactttggt agctttccag gaaaaaaaag ctggaaatta
     5401 tcgtgcttta cgtacacaca atgtgtctcc tatgacactt tggattaata aagttatagc
     5461 tatggcagtc tacagtttta tttcaacgtt agttttaata gtcgtaacaa ttataactgg
     5521 tttaatattg aaagctggtc cagttccttt tggacagatt ataggggcaa gtattgtatg
     5581 ctgggttgtt tctcttgcta tacttccact tcagctatgg ctagctacat ggaaaggtat
     5641 gttcctaagt atgggagttg gagctttagg tatgatattt ggtgtccttg ctgcgacaaa
     5701 accattttgg attgcagtac cttggagttg ggcagttaga atggtatgcc caatcatcaa
     5761 catacatcct aacggtacta tattagaagc tggagaccca cttttgaata cttcagtgat
     5821 tcctattggg attgttgttt ctttggttgt gtttatagta ttaacagcgc ttactgcagc
     5881 ttggttcaat aggagggatg acaaatgata aagctattat catcagaatg gcttcgagca
     5941 aagaggacag ctgtccagtg gcttactctt tgtatgccta ttattttttc attatgtgtg
     6001 gttttttatc tcacaactaa atctggaagt acacaagaat ttgcttttga aggatttttt
     6061 acagtatgga ctgtattcat cattcctatt ggagtaggta ttttagcagg atttatagtg
     6121 caggaggaag agcttgctgg aaattttaat ggttttttat gtgtaggtat ttctcgtgtt
     6181 agattatatc tgggaaaatt tctttttctt ctgttttgcc taactatatg cacatttatt
     6241 gctacgttga tactttgcat aggaatgagt attgcagtgc catctggagc aacaatatgg
     6301 ttattcctgt cagcagcagg acttgtggtg attgggacac ttccactatt agctattcat
     6361 ctttgggtga gttttgcatg gggtatgggt gcatctattg gtattagtat cggtggaatt
     6421 ttgatggcag caatacttgg attaactagc gttggtgaaa aagtatgggc atttgttcca
     6481 tggacatggc cagtaaaact aggtatgctt ccaggaattt attttattaa agaagctgga
     6541 actatttcaa ctgaggcatt ttactctaaa gtgatgcaaa cagctagtat agagcttata
     6601 gtagtagcaa taggtttgat tatattcttg ataggaggcg ttatttggtt taaaatgtgg
     6661 gagggaagga aaagttatta gtagacgaat attgttttaa tactgacctt cccataagag
     6721 aaaggtggtg ttatattgag taaaatattg attattgatg atgaaatgga cctagtaatg
     6781 ctacttgagg atgaattgaa ggcaaaagga catgaagtgt tgatagctta tgatggtcag
     6841 gcaggtatag agttatcaaa acaggagcct gaccttatta tactggatat tatgatgcct
     6901 aaaatgaatg gatttgaggt atgtcaggca attagagatg atgttttatg cccaattata
     6961 tttttaagtg caaagcaatc agaaaccgac aaaatcaaag ggttgacctt tggaggggat
     7021 gattatgtaa caaagccatt tggactgcgt gaacttatgg caaggattga agctaatctg
     7081 cgtagggaaa agcgttcaca gtatatcaat gaagagaaca agagatctac tttgtatttt
     7141 gggaagttat gcttatatat gaaggaacgt gcaattaaaa ttgatggtaa gaatattgat
     7201 ttaacaaaaa tagagtatga tattgttgaa ttactagcac tccatgcagg acaagttttc
     7261 tcaagagaac aaatctatga aaaagtatgg gggtgtgatt ctgaaggtga ttcctcaact
     7321 gttgttgaac gagtaaaaaa aatacgagct aagttttctg ctgtgacacc agaaaaagaa
     7381 tatatttcca cagtttgggg tattgggtat aagtggaata aaatgtaaag agagagaggt
     7441 tattaaatga aaaatcaatc ccttattacc caatttagac atacttttat ttttattata
     7501 atagccagta tagttgccac agtgattact tatgtatttg ctctctattt gtatattcat
     7561 tccttaaata aggatatcta tccaccaaac tattatgagc gacaagttcc tagaattgaa
     7621 aaatacatca atgaaaaaaa cataactttg ttatcacaat cgaatgaaga aggtttaaaa
     7681 agaacgattc gtggggatga tatgccatat caagttgtgg ataataatgg caatattcta
     7741 tatggtacta accccaaaaa actatttaaa acaaaagaag aattatttaa taattttatc
     7801 aataaaactg ttcgtaaagg tggttatatc catactgttc caatcaaagg ggataatgga
     7861 aagattgagg gagctgttat tctattctat caagtcaaaa taacctttgc aaatagtaga
     7921 gggagatttg tatttgcagt tattataatg gctctgtttt ctccatttat atatatcgtg
     7981 ggttttacta gatggctttc taagcgcttt gtaaagaata tcaatcaacc attacatcta
     8041 ttgattgatg cgtccaagaa gataaaagaa aaggaccttg attttgaaat cgactattat
     8101 tcagataatg aactcggtaa actttgtagt gccttttctg aaatgaaaga cgaattaaaa
     8161 ggttctttat ctgcgcagtg gaagatggag caggaacgtg ttgaaatggt ggaagctcta
     8221 gcacatgatt taaaatctcc tctttctatt attttgggat atacagatgc acttattggt
     8281 aataatacag atgacaatga aaaacttcat aggtatctta cagtgataag agaaaataca
     8341 gaaaaaagtg ctgctcttgt ccaaaagatg caatatacgt ctgacttaga aaagtcaaat
     8401 atacaattaa atcttgttcc tataaattta cctgaatttt tgaggcaaaa ggtgcaagac
     8461 tatgaattac aggctcatca aaaggaagtt gaacttatat tgaaaatgaa ggggaatata
     8521 caatccccaa ttcaaatcga cgtggatagg ttgacacgaa tttttgataa tatcatatcc
     8581 aacagtctgc aatacactcc atcaggtggt aatattagta ttactgtaaa ggatgaaaaa
     8641 aattgtattt cttatgagat ttgtgattct ggtagagggt ttagctctaa agatttaaaa
     8701 aaggctcttg ataagtttta tcgaggagat gaagctaggc agacaaaagg tggacattct
     8761 ggtcttgggc tttacattgt caaacaatta gtggaacagc ttggaggttc tgttaaaata
     8821 gaaaattcaa aatcaggtgg agcatgtgtg aaattttggc atagcattta actatatatt
     8881 ttaaattaaa ggggagtgtc tcaaagcgaa atttttaatt cgtgaggcac tctttttttg
     8941 tgaagttaaa tatattttca ttattccgac aatgaaaaaa ggcttatctc tattttgaga
     9001 caggcctttt ttaaagctta tattatagtt aaaaagattt attagttaga tttgaaagaa
     9061 aatagagaca gtcattgata atttaattta tattattatc tgtaaaatag ttataaattg
     9121 atatctatac agtcaattat attcatcttt ttaacttttc ttaacggaat aagagtagct
     9181 ataatacatg caagtacaga aataatagta gcttgtaaaa ttgaagtaat tggtatatta
     9241 gtgaaatcaa gcttttctat agttgccatg tttataataa tagcactggc atacccagca
     9301 attgaaccaa atatagcagc aaaaattcca taataaacac cttcccataa aaacatttta
     9361 tataagctaa tattactcat accaatagct cttttaactc caatctcatt agtcctagta
     9421 tttatatttg tatgagttgt gttaatgatg tttaaagtac caatcaatcc cacaaataat
     9481 ataactgccc atgctagaaa ttctatttgt ttaaaactct ctttaagttg cttatctgat
     9541 tcaatatatg atatccaatg agagccagga ttattttcac aaatctgttc aatagacaat
     9601 cttatttgtt ctaattttga tttatcttta gcataaatat ttatctggtt gaacttgttt
     9661 ttatttgtca aggtgttata aacagaatta tatacaataa catcaacacc atttatccag
     9721 ccattacctt gtaaaaagaa cattttatct actgtactat atatatctag ttgttttttg
     9781 ttgattgtga tactatcttt aggttttaaa tttgtatgtc tagtttttac acctggtgta
     9841 gaaatagcaa tcggattttt tataaaacag atagaaccat ttttaaagtc ttctagtata
     9901 gaatctgtaa tagaaggcat taagtctttt aaagtctgct cgtcaattcc aatgattttt
     9961 aacatctcac cactattttc aaaatttata tctgtatcta tttcaccctg tttgtattca
    10021 gagtacttta taaaggaagt gcgattgacg tttttcattt tatctatttt atctagtgtt
    10081 gatttatcaa aaccagatat ttcatttgtt aaggaaaaat ctccttcttt taaattgctt
    10141 actgacctag atacatctaa tacattagag aactcactca atgcaacgaa tactgttata
    10201 ctcataaata atgatagtat agttattata gttcttcctt tatttctttt taagtttagc
    10261 catgctagat gtctttcaaa gttttttatt gctttttctc ttttaatttt tcttttgatt
    10321 ttggtagtac taccagccat agcaattttg ggagagacac gtgaagcata tattgctgat
    10381 ggtaatgctg aaataaatga aaaaatcaaa gatacattag tgcttaatac taatgggaac
    10441 atatatgctg ttgtattttt atgtataagc tcagtaattt ctttattatc atttgctaga
    10501 agtatatctg gatttaagat attagtgacc attccagtta tgcctttaga agagataatt
    10561 ccaattacag ctccaatagg tatggcaaca gtacataaaa ttaagatttg taaaataact
    10621 atcttatata tctggtttgg ttcagctcct attgctctta aacatccata ctctttaatt
    10681 ttctttgtaa cagagatttt taaaatatta tagattacaa gtccagaagc cagtaaaacc
    10741 aataaggcaa caaataaaga taccaatata atcatagata taccatcact attagaacta
    10801 ttttcatctt tttcaaactg tatcttaaga gcatttaagt aaatccaatt atattgaatt
    10861 gaattgtgag aaagatttat ttttttagct aattgattta ctatttcttg aaacttttct
    10921 tgctgtttta ttttaaaatc caaagaggat aaaatatatc tcttaggtaa tagattttct
    10981 gatgtacctt tacctacaat accatttact ataccactta catatcctgt ataatcatcc
    11041 tctaaaatgc cactcaattt aaagttagct gtgtaattat atggtggtat ggtatcattt
    11101 aaaagtgaaa tatctaaatt cataggtata gtcactccta gtcttggttt tatacccata
    11161 agctttagag tgttttcatc taaggctatt tcgtttttat ctttaggcaa atgtccagat
    11221 tttaatttca ttaattttgg gtagttagaa agtcctgttt tatcatactc ttttacaagt
    11281 acagagatac cagaatcctt tattttacta ctgccaatag ttattgaagt accagcttga
    11341 taaactctat catctttctt tatgtcatca acttgatttt tagtaagttg gtgaaaggat
    11401 acatgtctat caccattaaa agaacgggct tgttcaattc ttaaattttg taggatacca
    11461 attgactgtc caaccacagt tgtcataata gttgatagta ctattgctat aatgatgaat
    11521 gtagtcataa gtttctggct ttttaattct tttattgaaa gtgaaaggta attcttcatt
    11581 tctatttcac ctccataaga acaccatcaa taattgaaat ttttctatca gcaagttttg
    11641 caattctatc atcatgagta attaccacta aagtctttcc aaactgtttt gaagttttta
    11701 taagaagatt cataacttca ctgccacttt tactgtccaa gtttcctgta ggctcatcag
    11761 caaatattat atctggtttt gcaataagtg ctctagcgat tgcaactctt tgttgctgac
    11821 cgcctgaaag ttcatgggga agatgattga gtctatctgc aattccaagt aatgatgaaa
    11881 tttcatttaa atagtcttta tcaggttgtt ttttatctag aagtacaggc attgaaatat
    11941 tttcagatgc tgtaagaaca ggtaaaagat taaattgttg aaagacgaat ccaaaggatt
    12001 ttcttctaag tgcagatagt tctttttcag tgtgtttata tatatcttta tcatcaagta
    12061 acacttgtcc agaggtagga ttatctaatc cacttaaaat atgtaaaagt gtgctttttc
    12121 cacttccaga aggtccaatt acagaaacaa attcacctga gtttattttt aaatttacat
    12181 tattaagtgc tataacttta ttgttattag aaccatatat ttttgataat tgttttgctt
    12241 ttataatcat aaaaaacact ctccttttct atataagaag agtgtaagtt ttaaaactca
    12301 attttttccc aacttttagc ttaaatctaa aaagtcttca ctatttacgg ataattcgca
    12361 gtgctgataa gcagtttcag tatcattttt aatttaaaat gtaatttatg cactaaaggc
    12421 tttacaatac aactctattg ctttaaaact attttacaca taaacttgtg gataattttt
    12481 tatgttttac ttcctcatta ttatataaat tatttttgtc acaaattcta agttgtcaca
    12541 taaatatact ttttatatca cgatttaatt cttttgattt tatcataatt gctatttctt
    12601 caaaagaaga aagaaaagcc acgatacata tcataattcc tgcaacatca agaccaaagc
    12661 aaatatagag taatggaaaa gcaaagatag aaattccagt gattttattc atcaaagtat
    12721 gaagcgatga aaatgtgcga tacttaaaaa aaccaatact gtaactaatc aaccgtaata
    12781 atgctattgc aacaatacac caccatagat acatgggaag agcaatattt tttccgacag
    12841 taaccaaaat tgtaaccgca aaaatgaaat cagcaatact atctaacttt gcacctattc
    12901 cactttgttg tctcaacttt cgggcaataa gaccatcaag tatatcacta attccacacc
    12961 aaaaataaca tatccagaaa ggcagtgaac agaatgtaga actaagcatt agcaatgcaa
    13021 acacaatacg agagaatgta atattattag caaaactttt aatataatca cctccaaaat
    13081 tctagtttca tactattcta catatttaaa tatatatttc tattattgtt tcaaatgcct
    13141 tttgccaaaa tattataatt gaagcaactc ttacaacttc tacaaaatag tatatatatt
    13201 ggttgtaaat tgaaatacat tttataaatt ctaaactaac aaagcttata taagaacaat
    13261 gaaagcttac aaaatcattt taatttcttg ttgagaattt agcagtaatt ttagcactgt
    13321 tagtagaatt attttcaata gaaatagagc caccatgttt ttcacataat atttttgaga
    13381 tatgaagacc cataccaaag tgtggattgc tgtcatttaa agtcttatct ctatagaaag
    13441 gtcttaaagc taattttaga gattccttac taaatcctat accatcatct tcaattacaa
    13501 aggtaaggag ttcttgttca agatatataa gtatggatac attatttcta gcatatctaa
    13561 aagcattcga tattatattt tcgacgactc taagaatgat attttcatca aaaaatagat
    13621 ttatattatt tatttgactt ttaagtgtaa aagattttcc ttcttgttta gcaatgatag
    13681 atatattttc atctaagttt tctataaact tgtttatatt cgacatagat tttgaaacaa
    13741 ctaaatcttc taatcgttga gcattactca tattttctac atagtattca attctgttaa
    13801 tatgctcaga cataagttgt gtggttgata aaatcttctc atcactaagt ttgcctgttg
    13861 gtatgtattt tgttaaatag tttgagtaac cttttagaac tgttagggga tttcttaaat
    13921 catgagaaaa agcagcatta agttgttttc tatcattaat catagtccac atatttatgt
    13981 tgtttttatt tagttcagac ctcatttttt caaaagcatt acatagatta cctaattcat
    14041 cattattatt atattctaag caaaaatcta aattgttgtt ggatatttcc attgcacctt
    14101 tattgaggat ttctatgggt gtttttaatt ttaccttgta aaaaattata tcagctaata
    14161 ataaaagtcc tataaaaaat ataagtggaa gtacaagctg aagtatgttg attatattaa
    14221 gtaatgtttt atcataatca ctaaattggt tcacattgtt ttgaactatg gttataatac
    14281 ctgtatttat aggtgttttt aaatacttat cttgtataga attatataga ttattaagta
    14341 aaatgtatgc gaaagtagta agtactgatg ctataattag tgaagataaa gtaagaacaa
    14401 agaaagcttt ttttaatgac atattactta ttttatccat ttataaccaa ctccccagac
    14461 tgtttctata taattttcaa gagtatgttt tgatagtttg attcgtattt ttctaatgtg
    14521 ttccattata acatcactat ttccattgct attgtatccc cagactctat catatatttt
    14581 ttctttatca aatacctgtc caggattcat agagagaagc tctactatat caaattcctt
    14641 ttttgaaagt gaaatctgat tattatatat acaaattaac cttttttcgt aatctattgt
    14701 taaatcatta taaaatttaa ttttagtttg atttgacttc ctttgttcac gtcttaaatg
    14761 tgcagatact ctagctccaa gttcgtctaa atcaaaaggt tttacaatat aatcatcagc
    14821 accagcacga aaaccattta tcttgtcagt attttcaatt ctggctgtaa gaaatagaat
    14881 agggcatgat acataatctc taattctatt acaaacttca attccatcta tttcaggcat
    14941 gtttatatca agtagaatga tatctggatt tttggatatt ttttttaatg agtcttttcc
    15001 actttgagca gtgtaaacct cataaccatt catttcaaag taatctttta agagtgttat
    15061 tataccttcc tcatcatcta cgacgagtat tttattgttc ataagaatgc tcccttctaa
    15121 attaagtttg tatagtattt atattttagt ataaaaatct caataaattc tcaaattctt
    15181 agttatattt caaaacttaa tcattaaagt tagtatattt ttatatatta aataaatcaa
    15241 ggatgaaatt attagaaatt ttaatagaat atatatttga tatttaatat ttattggaat
    15301 gcaaaaacac agctgatgaa atcttataat ttaagttgaa aatggataaa tagaaaggaa
    15361 ggattttaat taaaaaggta gattaattaa aaagtatata aattataaaa atgaattaat
    15421 ctacattaat tagttgatat atcaattatt attatttttt cagttgctta aaatatactt
    15481 gatatatcaa ctaatgttgt tttttaatta aaagaaattt taggaaagat aatattaact
    15541 aggagagata atatcaacat tgaagaaggt agagcaatat ttttacataa ttgtgaacat
    15601 tatctggcat gtgtgcattg gtgttttaag caagcaattc aatagaaaga tgtgactaaa
    15661 aatagaaaga aataccataa tccacatgta aagctagaag acataataag taaaaaaagt
    15721 aaagatattt aataaaagaa ataactaata gtgattaaat tagtaaatgc tgacaattag
    15781 cataatcatt agtaattatt agtattattt aattaaatta attttagcat tatttttatt
    15841 aaattaattt taaatcaaat taatgagaac cccataaatt tttttaacac ttaatttatg
    15901 atttatgggg ttatgttctg ttttatttca aagtattaag ttcatataaa tcagttcttc
    15961 tgtgcttaag aagtggtaac tgttgtctta tgtcaggaat taaatctcta tcaatgtcag
    16021 cataaataat acattctttt tcatcagctt gagcaattat tcttccccaa gggtctgaaa
    16081 ttcttgaatt tccaaaagct atataagaag cattcatatt tctagcaggt gcagcaccaa
    16141 catagaatat ctgattatct aaagcacgca ttcttataga taactcccaa tgggcgggac
    16201 cagtagtcat attaaatgca gcaggtaaga taacgatttc aacaccttta agagccatta
    16261 aacgtgataa ctcagggaaa cgaatatcat agcaaatagc tataccaact tttccatatt
    16321 cggtatctat tacagtgaca tcatttccag ctgtaagagt atctgattct ttaaaactaa
    16381 cttttcctgt aacttcaatg tcaaataagt gcattttacg atgttttcca attagagctc
    16441 catttttatc aaatacatag caagtattat agatttttcc atattctaac tcaggaatac
    16501 taccagaaac tagataaatt ccatgtttct cagctaattt actcaatatc gttgttgttt
    16561 cacctggata ttcttcagca aatttaggaa aatatttatt ttcatatggg cagttaaaca
    16621 tttctggcaa aactataatt ttagctccat tgatggcagc ttctgttacc atatctacag
    16681 catgattgat attttcaatt ttattttctg tggtcatcat ctgacatact gcaattttat
    16741 agaaattcat agaaattact cctttcaata tttttatgtt attataacat attaaataaa
    16801 aattagatat tcttagattg acctaaatgg gtatggagag aagtgaataa gaatattaaa
    16861 aggtatataa aagttatttt tattatagat ggaacccata tattgacttt ttttatatat
    16921 gttgatagta ttattataga taaaatagaa taattaatag agttttatag ggttttgcaa
    16981 ttattaatga ttggcctggt ccgagataaa acccacaaca tagtttttgt tgtgaacacg
    17041 gaaggacaaa aacctgggag atattttata atatttctca ggttatttta ttggaaaaat
    17101 aagagataca tttgaaaaga gggatgagtt tatgaaatgg ttatttttgt ttgttgcagg
    17161 tttgtttgaa gtgttttggg cttttcaact aaaaaattca catggatttt ctaaattaac
    17221 tccaagtata cttactgttg ttggaatgat tgtaagtttt tattttttgt cattagcact
    17281 aaaacatctt ccacttggaa cttcatatgc aatatggact ggaattggta ccgtcggtac
    17341 agctattgta ggcatggcta tcttaaatga gcctattagt atatcaagaa ttgcatgtat
    17401 aggctttatt gtggtgggaa ttgtagggtt aaagctatta tctactaatt agagtatatt
    17461 aatttaaata agctatgaaa ttaaaggcga gaaaatgttt aaaaataatc aacatttaaa
    17521 aagtctttac ttcatagctt atcttattta taagtttatt atatttgaaa attatatgta
    17581 tatactattt atctaataaa agagcagtta aatccatagg gtcaactatt acaccatctt
    17641 tataaacacg catatttcca gatgctattt catctattaa gattacttca tcattgtaga
    17701 atccaaattc aaatttgata tcataaagtt caagaccttt ttcagctata gtatcacgaa
    17761 ctatattaga aattttttga gtcatgcttt tcattgaatc aaattgagct tgagtcataa
    17821 tgtttaaagc ttctaaacct tcagatgtta taagagggtc acaacggtca tcatctttta
    17881 aagttgcttc tacatagcaa tctagtttag caccttcttc aacacatgaa ccataacgac
    17941 gtaagaaact accaacagct ctataacgac aaataacttc taatccatta ccaaaaactt
    18001 ttgctggtag aacttccata gttgcttctt ctaaattagc acttacataa tgagttttaa
    18061 ttccagcatt gtttagaatt tcaaagaact taacagatgt ctctaaatta gctctaccaa
    18121 ttccatctat acttagacct acactatttt cgcctgggtc aaatactcca tctttaccag
    18181 ttacatcgtc tttaaattta agtaagtaat ttccattatc taattcaaaa acatctttag
    18241 tctttccttg atacactttt ttcataaaca acctccaaaa agttaaatta ttatattgaa
    18301 taaggcatta gtgtcaaaaa agttctgata ttcgcatgaa aatttatatt gataccttat
    18361 atttaatatt atatcacaaa tagaagggaa atttccgaac aattttataa aaataacaaa
    18421 aaaagtacat tttataaaac ttataataag agaactaata taaaatgtta gtgtcgttta
    18481 tattgacaca cataagtttt aatataagaa acttttgaca cactttgaca cacatttaat
    18541 gataatttta taaaatgcat gaatgcaatg cttacatata ttggcatgaa agttgcttta
    18601 gatatttaac atataaacaa tagggatata ttctcaaata aaaagtaggg gtggttaata
    18661 gtgaagtcag ttagattcta tggtataaga gacacaagag tggaggatgt ggatgtacca
    18721 aaaatattag aaaaagatga tgtaataatt aaagtaaaag tagctggaat atgtggttct
    18781 gatatatcaa aatattcaaa gacaggtcca catatggttg gagaaatttt agggcatgaa
    18841 ttttctggag aagtggcaca ggttggtaaa gaagtaagga gttttaaaat aggagataga
    18901 gttgctgttt gtccagctat gccatgtttt gaatgtgatg aatgtaaaaa aggattgtat
    18961 tcaagatgta ataatgtagc aataatagga aataaagaac ttggaggttg ttttgcagag
    19021 tatacaaagg tgaaagagag gaatttaata aaaataccag atgagataag ttatgagact
    19081 gccgctgcgc tagaaccagt atgtatagca gggcatggtc tttttagatc agaagctaaa
    19141 gttggagata ctgttgtagt acttggtaca ggtacaatag gattattttc aatacaatgg
    19201 gcaaagatat ttggctctac aaaaataata gctgtagatg tatttgatga gaagttagac
    19261 ttggcgaaag agttaggggc agatatttgt ataaatgcaa aagaaaagaa tattgtagaa
    19321 gaaataaaaa ggttaactga tggagatggt gcagatattg ttattgaatc ggctgggact
    19381 ccaatgacat gtgggcaagt attgttgtta gctaaaaagg gagggacagt attgtatgca
    19441 ggtgtaccat atggggatgt agctttgact agagaacaat ttgaaaagat agtaagaagt
    19501 gaattaactg tcaagggaac ttggtttgga aactcttttc cattcccagg aaaagaatgg
    19561 agtgctggat tgtatcatat gcaaaagggt gatatgaatg ttgaaaaatt agtaacacat
    19621 agaataaatt tagaagaagt accagcttat tttgaaaaag tatacaaaag ggatattttc
    19681 tttggtaaga taatgattaa tatagataat tagattagag tactaaataa taatccatat
    19741 aaacaaggtt aagaaaggta tgattcatat gacagggata attatagcca cacatggaaa
    19801 tatggctaaa gcaatgttag aaagtgctga attaatagta ggcaaacagg aaaatgtaga
    19861 aactttagga ttaaaccatg gtgacagcat agatgagttt agtagaaggt tagaagatag
    19921 aattgagaaa tataaggatg aaggggtttt agtattgctt gatttttatg gcgggactcc
    19981 atttaataca tctgctatta taataaataa atttgctaag gtatgtgagt tagaatgttt
    20041 gacaggtgta aatttaccta tgcttttaga gctattttta aacagagata ctatgaagtt
    20101 aaaagacttg aagaatttat gtgaggaagt tgggatatca ggaatcaaag atgttaaaac
    20161 tgttttaaag ttgtaaaata aattaaaaat ctggaggaat aattatgagt aatatagcat
    20221 taacaagaat tgatgataga ttaatacatg ggcaagttat tactgcttgg tgtaagatta
    20281 catcggcaaa aaggatagta atcgtggatg atttagtagt aaaggaccca tttatagttc
    20341 aagttttaca aatggcagct ccatcaactg ttaaggttga agtacatgat gtagaatcag
    20401 gtgctgaagt tttaaaatca cataatggag atgaacattt aatagtattg gtcaaatacc
    20461 ctaaaactgt cttaggttta gtaaatagtg gggtagattt aaaggaattg aatgttggag
    20521 gtatgggagc agggcttggt agaaaaagtt tttataaaaa tatatctgtg tcagatgaag
    20581 aaaaagaaat atttagagaa ctaatatcaa aagatgttaa atgctttata caaatagtac
    20641 cagatgcaaa gaaaattgat gtaggaacat tattataaaa aagactatct tgtaaatctt
    20701 taaaagaaaa tagggaggaa gaatatgcac atttcactaa ttcaagcagt attaatagca
    20761 attttttatt atctttcttg gagtccgtgg ttgacctatg ttggattttt tacatggaat
    20821 agacctctat tggctggatt tgtaacggga attatattag gagaccctgt tcaaggagca
    20881 attataggag caggtataaa tatgatatat ctaggtttta tatcagcagg gggagctcag
    20941 atgggggacc cagcttttgc aggatatgta ggtactgctt tagctatagc atctaagcta
    21001 gatgtaagta ctgcaatggc aatagctgta ccattgggaa ctgttgcaac tgtactttgg
    21061 attggaaaaa tgacagtaaa ttcttttttt gcacattggg ctgatagaga ggttcaaaaa
    21121 gggaatgttg atagagtagc atttataaat atagtaccac cacagatact gttatttgca
    21181 atgagtttta taccagcatt attagttgtt tattttggtc caaatgcgat agatggtatg
    21241 ttggaagtaa tgaatgagaa tgtgttacat gtatttaatg taattggggc tatgttgcca
    21301 gcacttggga ttgctatgaa tttaaaattg attggaaaca agtttactat gccctttttc
    21361 atattaggaa tactaatggc tgtttatttt aaagtagata taatagtaat atctgttatt
    21421 ggagtaatac ttgctcttac aattacttca attaagtacg gaaaagatag tgctacatct
    21481 taaatacatg tgagggggaa aatattatgt cagatataaa aaatgaagta tctaagaaaa
    21541 cgttaagcaa aaaagatgta ataaaatcat ggttaagatg gtttttcttt gcgcaatcta
    21601 attacaatta tgaaagattg caatcaacag ctttctcaca ttcaatgttg ccagtactta
    21661 aaaaattata tccagataag gaagagttaa aacaagaagt ggagactcat ttagcatttt
    21721 ttaacacaga acctatatgt ggatgtgtta tacatggaat aacaatagct atggaagaag
    21781 aaaaagctaa tggagcagat gaaataagtg gagatggtat gaatgctatc aaaacaggct
    21841 tgatgggtcc actagcagga attggtgata ctttaactca aggagttata actcctatag
    21901 tgttagctgt ttgtattggt ttaactgagg gaggagcatc aattgcaggt cctatattat
    21961 ttgtaatagc tcaatatata ataatgactt caatttcttt tggaatgtgg acaaatggat
    22021 ataagtatgg gaaaaaagct gttgaaagta tacttcaagg tggaatagta aataaggtta
    22081 tagaaggggc ttctatatta ggtactttag tcatgggggg acttgtaggt aggtttatta
    22141 atttatctac accaataagt tatactagtg gtgattttaa atttagtctt caaacggatt
    22201 tacttgataa gatatttcca ggattaattc ctttggtact tactttatta gtactctttg
    22261 ctttaaaaaa aggcctttct ccaataaaaa taatgtttat actgatagta gtgggagcta
    22321 ttacaggtat tttgggacta ttttaaataa atatatatgt tggtgtgtaa attaggattc
    22381 tatatctatt gattatattt gtaagatgtt ggaaaattta tattaaaaag tttagcaatt
    22441 agggtaattt tataatagat taaattttaa acttatataa gctatatata gtatttaata
    22501 ttttaaaaag tatctcagaa agatttttaa tcagaatgag gtgctttttc atttgcatat
    22561 aaaatattaa gtaatttcaa aaaaatataa atttatttaa tttaattaaa atattataat
    22621 atattagtta catattaaaa tatataatcg agatattgtt aagacattgt cgagatatta
    22681 taatatataa ctaatgtatt ataaatagtt gtaaacttcc tgtaacatat attttatata
    22741 attaaagtaa tggatataat atgaaatata ataatgaatt ttagggatgt gaataaatga
    22801 acatattaat tgctgatgat gaaattagta tgcttaagat actttgtgca tattttaaaa
    22861 aagaaaactt caatgtgttt actgcaaaaa atggggaaga ggctttagat attttttatg
    22921 aaaataagat agacttagct atattagact ggatgatgcc aataattgat ggaatagagg
    22981 tttgtaaaga gataaaggaa aatagtaata caagggtact tatgttgact gccaaaagtc
    23041 aaagtgaaga tgaaatagaa gcactcaaca ttggagcaga tgaatatata aaaaaaccat
    23101 ttgatcctag agttttaata ataagagcta agaaactaat aaattataaa aatacaatta
    23161 atattaaaga tttaaaaata gattttgggt caagtaaagt ttttaaaggt gataaggaat
    23221 tattattgac taagaaagaa ttagaattaa ttcgttgttt aatcaataat aaaggaatgg
    23281 ttttatctag ggaaaaactt ttagatttag tatggggatt ggattatgag ggagatttta
    23341 gaacagtaga tactcatata agaagattaa gggtaaaaat aggagaagat ataataaaaa
    23401 cttatagagg attagggtat agtctggagg attttaatga ctaaactaaa caaaaaattg
    23461 tccataagta tatctatagt agttgtagtt gtttatataa tatctataat tataaatagt
    23521 atgtttattc atagatatta tttacatgaa aaaagaaata tattggataa tgttgaaaat
    23581 gaaattaaag ataaagacat agataaatta aaatcagaca ttactttaat tgaagaaaaa
    23641 aataatatag atatcgtata tatagaatta gataaaaatc ataaagataa aggtaatata
    23701 gataaaataa atcaagattt attaaatgca ttttggaaaa aaggtttaag tttaaataag
    23761 ttttggattg aagaaaatag tttaaataat attgataaga aaagtataaa taagatatac
    23821 aatcaaggta aaacaaaata tagtttgctt gtaaaattta tcaagaagga taattatttg
    23881 tttgctatat ctatacctat agaacattct gaagaaacta ttggcattgt aaatagattt
    23941 aatataataa tgggaatttt ttcagtagtt ataataacta tacttacatt tatattatca
    24001 aacagagtta taaaacctat ggaaaggtta aagtttttat ctaaagatat atctgaatta
    24061 aattttagga ctgaagatat aaaaacgaac gatgaaatag aagagctagc ttacagcata
    24121 aacataatga gcgaaaaact tgaaaaagca cataatgaat tgaataaaag aaatgagaat
    24181 ctaaaaactt ttatatcaga tgcatctcat gaaatgaaaa ctccaatagc acttataaaa
    24241 gcatatgcaa taggtatgaa agatggtttg gatgatggga cttatacaga tacaataatt
    24301 gaacaagcag aaaatatgac caatacaata aattctcttt tatattgggc aaaatatgag
    24361 aagaaggaga gaagtctgtg tctggtagat ttaaaggaaa ggttatataa aagtttaaaa
    24421 aactatgagc tgttgataga aagaggtagt ataagtgtta aatgcagtgt agatgataaa
    24481 gaacttataa taagttcgga tgaagatagt atagatatgg tatttaataa tttgattagt
    24541 aatgctataa agtatactaa taataatgaa atagagatta atctttttga agagaatgag
    24601 agaattatat tatccataaa aaatggtata gattacaata tgggagatga tatagaaaat
    24661 atgtggaagc ctttttatgt tttagagaag tcgagaagta aggagctttc tggtactggt
    24721 cttggtctta ccatagttag aactatactt gaagaaaata attttagata cagggttgaa
    24781 atttgcgatg gaatt
//
LOCUS       DAELSY010000049        24601 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.049, whole genome shotgun sequence.
ACCESSION   DAELSY010000049 DAELSY010000000
VERSION     DAELSY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24601
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.049"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            161..1171
                     /locus_tag="KPG09_001737"
     CDS             161..1171
                     /locus_tag="KPG09_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG0966354.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEESTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            1307..1795
                     /locus_tag="KPG09_001738"
     CDS             1307..1795
                     /locus_tag="KPG09_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0966355.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            1971..3899
                     /locus_tag="KPG09_001739"
     CDS             1971..3899
                     /locus_tag="KPG09_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0966356.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFILSNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFENLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            4074..4484
                     /locus_tag="KPG09_001740"
     CDS             4074..4484
                     /locus_tag="KPG09_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBG0966357.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            4506..4808
                     /locus_tag="KPG09_001741"
     CDS             4506..4808
                     /locus_tag="KPG09_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBG0966358.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(5024..5716)
                     /locus_tag="KPG09_001742"
     CDS             complement(5024..5716)
                     /locus_tag="KPG09_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG0966359.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMMAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(5742..6929)
                     /locus_tag="KPG09_001743"
     CDS             complement(5742..6929)
                     /locus_tag="KPG09_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0966360.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRIPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSISYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            7265..9142
                     /locus_tag="KPG09_001744"
     CDS             7265..9142
                     /locus_tag="KPG09_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0966361.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKTVYDEVIKIVPQSNDVGKKNESQEKQSIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSFIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            9454..10860
                     /locus_tag="KPG09_001745"
     CDS             9454..10860
                     /locus_tag="KPG09_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG0966362.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNTVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            10888..11736
                     /locus_tag="KPG09_001746"
     CDS             10888..11736
                     /locus_tag="KPG09_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0966363.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            12397..13089
                     /locus_tag="KPG09_001747"
     CDS             12397..13089
                     /locus_tag="KPG09_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0966364.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            13086..14021
                     /locus_tag="KPG09_001748"
     CDS             13086..14021
                     /locus_tag="KPG09_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0966365.1"
                     /translation="MKMDEQTIILFLFLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGNENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVDDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTVFTVSLPLI"
     gene            14169..15092
                     /locus_tag="KPG09_001749"
     CDS             14169..15092
                     /locus_tag="KPG09_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0966366.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRKFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAHAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            15076..15846
                     /locus_tag="KPG09_001750"
     CDS             15076..15846
                     /locus_tag="KPG09_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0966367.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVTVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            15856..16638
                     /locus_tag="KPG09_001751"
     CDS             15856..16638
                     /locus_tag="KPG09_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0966368.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGIAVLLSLFSFLCTFILGLLFCNINISVTLIVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            17015..17551
                     /locus_tag="KPG09_001752"
     CDS             17015..17551
                     /locus_tag="KPG09_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG0966369.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            17583..17972
                     /locus_tag="KPG09_001753"
     CDS             17583..17972
                     /locus_tag="KPG09_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG0966370.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            18009..18395
                     /locus_tag="KPG09_001754"
     CDS             18009..18395
                     /locus_tag="KPG09_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBG0966371.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGSQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            18522..19964
                     /locus_tag="KPG09_001755"
     CDS             18522..19964
                     /locus_tag="KPG09_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG0966372.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVENESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLIDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            20261..21076
                     /locus_tag="KPG09_001756"
     CDS             20261..21076
                     /locus_tag="KPG09_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0966373.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINNFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKRVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
     gene            21677..22861
                     /locus_tag="KPG09_001757"
     CDS             21677..22861
                     /locus_tag="KPG09_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966374.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(23238..24347)
                     /locus_tag="KPG09_001758"
     CDS             complement(23238..24347)
                     /locus_tag="KPG09_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0966375.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
BASE COUNT         9001 a         2917 c         4373 g         8310 t
ORIGIN      
        1 ggcattgtat actggaatta gagtaattaa tttatttttt aacatacaaa tgattttatg
       61 aaaaaaaaca agactcaaaa atttttatat tctttgatag aataaaattg agagtaaatt
      121 tattgaattt tataaaattt ataaggggtg agtctaagat atgcatgaag ataaaaatga
      181 tatatttgaa tcaatagata ctgaggattt tgataataaa acagaagaag aatgtttaga
      241 gctagtaaga aaaaatggat tgaatttaat atacataaaa aatcaaacag aagagatatg
      301 tctagaggct gttagacaaa attgtaatgc aataaaatgt gttcagaatc agacagaaaa
      361 aatatgtata gaagcagtta aacaagattg gagaatgtta gaatttgtgg aagagtcaac
      421 agaagaaatt tgcatgttgg ctattaacca agatggaaca gcattaaaat atatagaaaa
      481 tcagacagag gagttatgct taagagccgt tgaaaaaaat ggtgcggctt tggagtatgt
      541 aaaagaacaa acagaagaaa tatgtataga agcagttaga aacagcgaat ttggattggc
      601 aagagtgaaa aatcaaacag aaaaaatatg tatggaagca gttaagcatt gtagctataa
      661 tctaaaatgg gtagaaaatc aaacagaaga aatatgtatg gaagcagtga gacaaaatgg
      721 gcttgatttg aaatttgtaa aaaatcaaac agaaacaatc tgtttaagag cagtaagaca
      781 aaatggaatg gcattagaat ttgtaaagga acagactgtt ggtatatgct taaaggcagt
      841 aagacaatat ggaatggcat taaaatttgt aaaagaacaa acagaagaaa tttgtacaga
      901 agctataaaa caagataaaa gagcacttag ttttgtaaaa ggagataagg aaaaatacaa
      961 ggctctatat gataataatg aaccatttgc aaagagatat gttagaaatg taatagaaaa
     1021 agaaaacaga gcattgatta aaaaaggaga agaaaatgca aaaatcaaaa ttgcaggaaa
     1081 actttatgat agaggcatgt cttttgaaga tatatcagat atagttgaga ttgaaataag
     1141 caagttaaaa gtgagtttag gtgtttttta aagtaataaa tgtaggtgtt ttttatggga
     1201 aatagttaat aaaaaaataa tcgactagct ctttgtagga cagcaaaatt agtgatataa
     1261 tttgatgtat aataaattat ttttattaga taagggggaa ttaaagttga agattacagt
     1321 attataccaa acaagaagtg gaaacacaga aagggtcgct aagctgatag aagatggagc
     1381 taaaaaggtg gatggagtag atgtaaagct tatgagactt gataatattg atttagatta
     1441 tttaaatgaa agtaaagcag taatatttgg aacacctact tatttagcaa atacaacttg
     1501 ggaagtaaaa aaatggtttg atgaagattc aaaaaaagtt aatttagctg gaaaattagg
     1561 agctgttttt gctacatgtg attatatatg tggaggtcca gatgttgcga tacttactat
     1621 agtaggacat ttgatggtta aagggatgct tgtgtattct ggaggtggtt ctttaggaaa
     1681 gccatttata catttaggac atgtacattc aattgaaggt ccagagttac aagatgagaa
     1741 ggctataata tttggtgaaa gaattgctac taaggcaaaa gaactatttg cttaatttct
     1801 atatgcataa taatgattag agtaaattta gggttttaat ggagatttga agtaaaagtt
     1861 ataaaataaa aaattttcca taaaattact caagatgaat tataatataa ttataaataa
     1921 attatattat taaaatattt aaaatcaaac gtcaatagcg agggaagttt atggtatggc
     1981 atatagaata tgagattttt tctgcaatta tagttatttt tttaatggta tactttttta
     2041 gaagtaaatt tatacctact ttacaaaaca aaatatattg tgcactttta atattctcat
     2101 ttttatttat actttcaaat attttaggtt cattttgtct aaataatata gacaaaattc
     2161 caatttttat tactttttta ttgaatcaga tatacttatt gttattgcca ataccagcag
     2221 ctcttgtatc attttatgtt atggctatta tttatcaaga tataaggtat atgaaaaaaa
     2281 gattattatt tttatctata ccacttataa tttgtataat cttatctatt acaaatcctt
     2341 ttacgcatat tctatttgga ttgtcaaaag aaacaggtta tatacggggt tgtggatatg
     2401 ttgcaacttt tgcatctttt ttcttttatg caatatattc agcattttta tcttttaaat
     2461 atagaaaagc aatgcatgaa tcaaaaatat gggctattag aggttttctt atagtttcaa
     2521 ttgtggcaat tatcttacag gcaatttttt tacagtatct tcttacagga actgcatgtg
     2581 catgtagttt acttttgaca tatctttcta tgcagaatcg aggtcttatt attgatgacc
     2641 ttactggtgt actcaataga caatctttta ttcaagagct agatttgaat atcaatactg
     2701 aacaatcagg atttattatt acaattgcgc ttgatgattt taagtttatg aatgaaactt
     2761 ttggaacaaa aaatggagat attgcactaa aagaagttgg aaaatatttg attcagatac
     2821 atgatgtaaa ccatgtatat agaatagggg gagatatatt ttctattgtt gtcaatagtc
     2881 aattaggtat agaaccagat gatattatca gaaagataga agaaagattt aaaaagcctt
     2941 ggctagtaga agagataagt tttaatttag caactagtat tgctgttgtt tattacccag
     3001 aaaatgcaga aacgacagaa gatgtaattg ttgctataga tttctctata catgaagcaa
     3061 aacgttctga gtcaaggcga gttgtttatg cagatgcgtc tataagtgaa aagataaaga
     3121 agaaacatgt tattaaaaat tgtttaaaag aagcattaaa aaatgatggg tttgaagtgt
     3181 actaccaacc tatattttct aatagcaggg gcaggtttac ttcagcagag gcattactta
     3241 ggctagaaca caaagagtta ggttttatac ctcctgatga atttattcca attgcagaac
     3301 aaacaggatt gattaactca attggattag tagtctttga gaaggtatgt agatttatag
     3361 ctagtgatga gtttgaaaat ttaaaattgg ataatatagc tgtgaatctt tctgtagtac
     3421 aatgtatgca aaaaaactta gctgatgatt tactttatct tatgaataaa tataatgtat
     3481 caccaactaa atttaaactt gaaattactg agactgtggc tatgggctct tttaatataa
     3541 ttaaagaaac aatggaaaga ttaattgatt taggtgttaa gtttgcactt gatgattttg
     3601 gaattgctta ttctggagtt actaatatgc tttctttacc attttcagta attaagttag
     3661 ataagagttt gatttggtct atgaatgaag atagtaggca taagcttaca gttgagacga
     3721 ttatagctct tataaataaa ttgaatatga aggctgtagc agaaggtgtt gaaaccatta
     3781 agtgtgctga agatttaata gctatggagt gtgaatattt gcaagggtat tatttttcta
     3841 aaccagttcc agaagatgtt tttaaaaatt tgttgaaaga aaatactttt acaagttaac
     3901 accctttatt tttttaatta caactttgtt cattgacact gccagcgata tattataaaa
     3961 ttaacacaat aataaatatt aaatttatta tacatattat taatattaat taatggagta
     4021 aagatttatt atattttatt taattatcct aaagtgagaa aggaagatgt gacatgaata
     4081 aaaagaaaat attagtgcca attgatggga cagaaagaag tatgcattct ttggaattta
     4141 taaaaggaat atttaaaaaa gatgaagttg aagtagaaat aatgaatgta aaagaactag
     4201 tatttattga tggcatatcc ctggctgaag agataaaaaa ttctgaaaat ttaggaagaa
     4261 gaatcttgga taaagcagca gagataatgg gagagtatga tgttaaagta cattttactt
     4321 ttggatatcc aggagatgag ataattagaa aagcaaaaga agataatgta gattttatag
     4381 ttatgacaaa atctactaaa aaaggtctta caagaatgat aggttcagta acagcaagtg
     4441 tagttaaaca agcaaaatgt atagtgatga ttgtaccgga gtagaattag aaaggtattt
     4501 gaaatatgaa taaaaagata gtagcagttt gtgaaagtga aagaagttta aattctctta
     4561 agacagctgc aaatgattta ggatttgatt tagtttatga aattcaagaa aatgataata
     4621 taaaaaatga actttctatt tatgatatag aagatgcatc tatagtgtta tttgttacaa
     4681 gtgattctat agaaagcata gagaagattg atagatttat agatagagaa ttttatgaag
     4741 ttgatccaaa gtatataata gaggatgcaa aatctatagt aagtgaaatt ttaatagact
     4801 taaattaatc gtgggtgtcg cactaagaat agagcgacgc ctttttacat attttacata
     4861 agaaataaag ggctgtctca aactatagat aagactcttt ttcattgttg aaatgatgaa
     4921 aatatatttg atttcataca aaaaagagtg cctcataact aaaaagttca ctttgagaca
     4981 ctccattttt atatgttagt tgcatttaag gtaaaataga agtttaatca tgttgaaata
     5041 actgaattac atcttctaaa tgattgtaca ttgatgtata acctaagtaa gagtctttac
     5101 tatcaaatgc catcattatc ttgttatggt ggctaatcat atccttaaga gaatttctat
     5161 atttaaaacc gaagtattgg ttagaagaaa ctgttttagg tattaactca taatatttta
     5221 taaaaattgg attatgagaa gctaaaatta tctctttgtg aaattgtaag tcagcatcat
     5281 aacattcttc tatcaaacca tgagaaacct tatccaattc ctctattgac tttaggtaag
     5341 tatattttat tttttgaagt tctttagatg tagcatttaa aattgctagt ttagtagctt
     5401 cactttcgat aagttgtctt gctcttaaag attctacata aaattcttgt atgttatgat
     5461 ttttatagat ttcaccatca tcaaataggt caactttttt aaatccttca actataggac
     5521 ttgagtaagg cacagctcta agtattcctt ccttacagag tatatttatg gcatttctta
     5581 caggtattct acttatgtta aataggtcag caatctttct ctcacttggt aatttactat
     5641 taattttaaa agttccattt agaatgttta atttaatata atcaataatt ttttcagtgg
     5701 catttaagct tttcataaaa actcctaaac atataatttt tttatgattg tattatatca
     5761 tagttaagag ttaaatctac ttttttataa tatgtataag acaggataga ggccaaaata
     5821 gctataatag ccaagataaa gtatccatat tgtattgaag tatattccga tattttacca
     5881 actatcattg gaaatacaga cattcctata gcataaattg cttgaaaaaa acccattgca
     5941 gtagatttct gatgttttgg tacatttttc atagactcgc tagtaaggta agaaaggagt
     6001 atacctgtag atatacctgg tagcacttgt agtacaaata ttatattaat attttttatt
     6061 gttggaacta aaaagcaata ggctgaaact aaaataaaaa ctaaaggaat ccaaaatttt
     6121 ggcccatact tagtacaaaa tttagtagaa gcaaagccag aagaagcaac agcacacacc
     6181 atgtaagata tagaagatag acctatgagg gtagaactag aacctaagtt tttaagaatt
     6241 tgagtagtaa atgacattgt tgtagacatt tgaataccct gttgaattaa tgccataaag
     6301 gaaaaaaata caagtctttt atctttgcag atagataaat attttttaat tgatattttt
     6361 tcaggtattt tttttgaatt atgatttttt atatttaagg ctagaatcgt tccaataact
     6421 gaagacaata cactcatgat acataaggct gtcattccta atttttcata aaataaagta
     6481 ctcataacaa atccaatcaa cattcctaga ttgtttacca atattatcgt actcgtagct
     6541 ttttgttgat gttctgtaga aaaaaagttg gtatagtgaa ccatgtaaga aatccaagtt
     6601 gatgatgata ttccagataa aatgttagct ataagaaaac cattgcctcc aggagaaaag
     6661 actcttataa gagatgctaa accagcaaag aaaattccag acagtataaa tattttatgt
     6721 ttatttatag aatcagcaaa tactccaaca ggaatcctta ataaacattg tgagattcca
     6781 tatgcaccta ttattatacc aatcatattt gtagatacat tacatgcact taaatatgga
     6841 ttttggaaag gtatatatac atattgtgaa aaccagaaaa atgtaacaat agaaagtaaa
     6901 agtaattttt gattagatat ttttttcata ttatcctcca tatggtattt ttataatgca
     6961 aataatatac catttagatt ttttaatcaa gtactttctc tattaaaaaa aatttaaata
     7021 tataattatt atttacaaaa aaattatact ttatagttga attaagaaaa cgcttatgat
     7081 actataaagt taacaaattt gaatatataa cagtagacgt gttactggta atgcaggcaa
     7141 gatcgaatca atttttatag attatctata gagtactatg ggtataatct atttatttgg
     7201 ttcgattttt ttgtttaatt ggccataaaa caaaaagcct atagttgaaa atggaggatt
     7261 ttttatggat tataaaaata tagctcaaga aattctacta aatgtaggag gaaaagagaa
     7321 tgtaaatgaa gtgactcatt gtatgacaag acttagattt aaagtcaaaa gcgcttctaa
     7381 agtgaataag gataaattat ctaaaactga aggagtaata actgttgttg agagtatggg
     7441 tcaaatacaa gttgtaattg gaaataaagt aaaaacagta tatgatgagg ttataaaaat
     7501 tgtacctcaa tcaaatgacg taggtaaaaa gaatgaaagt caagaaaagc aaagtataat
     7561 taattctata ttatcagcag tagctggtat atttacacct acaatacctg ctatagcagg
     7621 ggttggtatg attaaaggta ttttatcagt tcttgctatg tattatatga ataaaaatgg
     7681 agtagatata aaagaaacgc aaagttatat tatcttaaat gccatggccg attctatatt
     7741 ctattttatg cctattatac taggatatac tgcggctaaa gtatttaatg caaataaaat
     7801 aatatctatg gtattaggag ctacactttg ttatccaact tttactgctt tgatggcagg
     7861 tgaagaatct gttagatttt taggattggc agtaacaaaa gctacgtata catcatctgt
     7921 tattccaata attattgcta tatgggctct ttcatacgtt gaaaaagtat tagaaaagta
     7981 tatacctgag gtaattaaaa taataatggt tccaacttta tcattggttg taatgttacc
     8041 tgcaacatta tttttatttg gaccaatcgg aatttatata ggaaatgtca taaacttttc
     8101 ttataagtat atttatgaat taagcccagc gttgtgtgga gcatttgtag gtgggttatg
     8161 gtgtgtgttg gttatatttg gtgctcatag agcattatta ccaataggta taagtgatgt
     8221 tgctcaaaca ggaagacaga atttattggc atttgcagga gctgcaaact tttcacaagc
     8281 aggggctgct ctaggagtat tctttaaaac taaaaatcaa ggactaaaaa caatttctat
     8341 gtctgcaact ataacagctt tatttggtat aactgaacct gcaatatacg gtgccaatct
     8401 tagattaaaa aaacctatgg tatgtgccgt aatatgtggt gcatttggag gtgcgataat
     8461 gggaatgggt ggagcttatg gtaatgcatt tgcaaaccaa ggtgttttaa ctatacctgt
     8521 atatgctgaa gcaggtgcat taggtttctt aagttatcta ggtggttgtg ctatagcatt
     8581 ttttggttca gctatttcaa cttatctagt tggatttgaa gatttggaag agtcttctaa
     8641 taaggaaaat tcatttataa aagtagaaac gaaggatggt gttattgata taacttcacc
     8701 tgtagagggc gaatgtatag aattgtcaga agtaaaggat gatgtatttg cttcaaaagc
     8761 tatgggagaa ggtatagctg tgttacctac aaaaggagta ataacagccc ctactgattg
     8821 tgaagtagct tctttatttc caacattaca tgcaatagga ttaaaattag ataatggagc
     8881 agaaatgtta atacatgttg gaatcaatac agtagagtta aatggtaaat actttacaaa
     8941 acatgttaat caaggagatt tagtgaaaaa aggtgataaa cttatatcgt ttgatataga
     9001 taaaataaaa aaggctggtt atgatgtaac tacgccagtt atagtaaaca atacatttga
     9061 ttttgggcaa gtagtttctt gtaaatctag ttatgtttca accaatgata atataatttc
     9121 attagtatta gctggtaatt aagttaattt gactagagat aagtaaattg atttaaaagg
     9181 aaataagtaa atcaacaatt aaatacatct aaaagtaagt ttattgcaat gaattgttaa
     9241 agaaaataat taatatggag taaagctatt gcaatgaagt aacaaagaaa ataattaata
     9301 tagagtaagt ttattgtaat gaattgttaa agaaaataat taatataggg taaatctatc
     9361 tcaatgaaat aacaaagaaa agagttaata tagagtgaac ttattgcaat gaatcaacaa
     9421 aaaaaataat caatataaaa aggagaaata tttatgaata caggatttcc aaaagacttt
     9481 ttatggggag catcatcttc tgcatttcaa gtggaaggag cttgggataa agacaataaa
     9541 ggaaaaaccg tagccgacta taattctttt aaaaagtctc atttacaggc agatactaaa
     9601 gttgcatcag acttttacca taattatgaa gaagatattg aattaatgaa agaattaggg
     9661 atgaaaacat atcgtttttc aatttcatgg gcaaggataa taccagatgg tgaaggagag
     9721 ataaaccaga aaggtttaga cttttataat aagataatag acaaactaat agagtgtgat
     9781 attgagccat tcgtaacttt ataccatttt gaccttccat ttaaattagt agagaaatat
     9841 aatggttggg aaagtagaga aactgtctat gcttttgaaa gatttgctaa aatatgcttt
     9901 aagcattttg gagatagggt aaagtattgg caacctcata atgaacagaa tttaattgtt
     9961 agagtggagg agagaattaa tatatatgat gaaacagatt catggaagat agataaaatg
    10021 cgtgctcaga tggattataa tctttgctta gcacatgcat tagcagtcaa tgcatgtcat
    10081 gagatgataa aagaaagtaa aattggagca gcagtatcat catctgtaac atatcctctt
    10141 acttctaagc cagaagatgt atatgcagct agaatgaatg ataattttaa agtgtactat
    10201 atgcttgata tgcatcatta tggagaatat ccagggtatt atatgaagta tcttgaaaaa
    10261 agaaatacag ttcctcacat ggaagatggg gataaagaga tacttaaaaa agctaaaatg
    10321 gattttatag ctgtaaatta ttataggaca aattgtgcag aagctttacc tgaagattca
    10381 caacatccat ttggattaag agaaggaact gttgatttta gtatgtatgg actatttaaa
    10441 atgtcaatga atcctaactt agaggcttct gagtatggag cagcaataga cccatcaggt
    10501 cttagagttg ccttaaatga atattggcaa agatatcact tacctgttat aataacagaa
    10561 aatggtctag gagctaaaga tatattagaa gatggaaaaa ttcatgatga ctatcgtata
    10621 gactatttaa gaagtcatat aaatgcatgt aaactagcta ttgaagatgg tgtagagatg
    10681 attggatatt gtccatggtc atttacagac cttttaagtt cttctcaagg ttttaataag
    10741 cgttatggac ttgtttatat aaatagaact gaccatgaag ttttagattt aaaaagaatc
    10801 aagaaagata gtttttactg gtataaagaa gttatagaga acaatggaat agtaaaataa
    10861 acataaatat atattgaggg ttttagtatg tacattacaa aggtattaaa taattcgttg
    10921 ctacttgcaa aggatgataa tgatgaagaa ataatattga tggggaaagg cattggccat
    10981 aattataagg ccgggtctga gttaaataaa aaagatatag aaaagatttt tgttcttcat
    11041 gatgaaaata taaaaaagag tattatacaa ttggcaacag aaatagatgc agaatatttt
    11101 ggtatagctc aaatgataat tgcatatgga atagaaaaat ataacttaaa acttatgaac
    11161 catatatatt tggccttaac tgaccacatt gcctttgcag tgagacgctt taaagaaggt
    11221 ataatgattg aaaatcacta tttgttcgaa atcaaggaat ttaatccaaa agagtatgat
    11281 ataggaaaat atgctattac tgtttttaag gaggttcttg gtttagacct accagaagaa
    11341 gagattggta atatagcagg ccattttata aatgcacaac aagataatcc ttatagtgat
    11401 agaaataaga gaagtgctaa aattgtaaat gctattttgc aaatagttca ttatcacttt
    11461 tctattgtat ataataaaga gtcattttat tataggagat ttgtaatgca tctaaaggcc
    11521 ttttcacaaa gatttctggc taatgaacca tcaagagaaa aaatagattt tatatatgag
    11581 caagttcaaa aaaattgtaa ggaagagtat gaatgtgtga aaaaggtagg agcttatatg
    11641 tataaggaat actccagaga attaccaaga caagaggagt tatatttgat gattcatatt
    11701 cataagattt taggagaatt atgcgaaaat agttagaata tcttaatatg ctagtaatat
    11761 tataaaaaat taaaaattaa ctacacttta aatatgaaca attgcattac ttaagggggt
    11821 tgtcttaaaa ttgatttttt taattaaatt tgaagctcaa ctcctttttt aaacctttat
    11881 atcattacaa ctttaatgtt tatacgcctg tactttagat gtatatctac tttttacatt
    11941 tctcaacatg caaacctacc attttttgca gtatgttaat tgaaataaac cattgagtta
    12001 accctaaatt tattctggac ttataggaat agttccaaag atactttaaa atattttctg
    12061 gaataattgc ttatttcaca tatgttttat tgtgatgttt tgatttaata aattcttcaa
    12121 ctctgtatat agtaaatgta ttatcatgtt tgttttattc aagcacactt ataatacgct
    12181 tgattttttc acaatgatga aatatactaa tgggtgttat aggtataaga atcgaattac
    12241 acttgaagaa gtaagtgata aatgagatat aaatcctcat atgataaact actatttcat
    12301 tgaggaacat attctaagtg atgtcaagat actacaatat gactaaattc ctagtgataa
    12361 aaaagtctac gaatgagtgt agaaagggga actaagatga ataaagtttt aattatagat
    12421 gatgataaag atctttgtat tttgttaaaa aagagtattt caattgaaca gattgaagca
    12481 aactattgcc attctgggag agatggactg gttgaacttt caaaacaaga ttatcagtta
    12541 gtagttctag acgtgatgat gccaggtatg gatggttttg aaactatgga acaaatacga
    12601 aaatatagca gtattcccat cttaatgctg acatcaaaaa gcgataatta cagcaaagtg
    12661 catggattgc gagctggtgc agatgactat ctaaccaaac catttgaaat tacagaattt
    12721 actgctcgtg tactgtcttt aatccgacgt tacacccgtt ttaatccaaa aaatgaggaa
    12781 ccacagcaac tttctttcaa aggtttgttg attgaccttg accagcatac agtgactacc
    12841 aatggcgaac ctatgttgct tccacccaag gaatttggtg tgcttcttta ctgtgcaagg
    12901 catcagggta agattttgac aaaacagcag atttatgaag ctgtgtgggg tgagccatat
    12961 gtatatgatg acagcaatat catggctatt atcagtcgcc taagaaaaaa aattgaacta
    13021 gacccatcat ctcccatcta tttgcaaaca ataaaaggaa ttgggtatca attcaacacg
    13081 gaggtatgaa aatggatgaa caaacgataa ttcttttctt attcttgctg tcgattggtc
    13141 tactttttct ttccatattt gctttttcca agcttgggca tattcacaaa cgcctaaaag
    13201 atatagagga gatactggct gatgtacaaa ttggaaacga aaatcgcaaa atactaatta
    13261 agccatgtga tgttatggct ccattggttt atcagcttaa tgagattgtc tacgattatg
    13321 aaaataagtt actttctctg aaaaagtcgg ataaagctag caaacaactt atgacaagtc
    13381 tatctcatga tgttcgtaca ccattaacaa ctctgattgg ttatctggat gctgtacaca
    13441 gtggtatagt tatcggacaa gaacgggagg aataccttga aatcgctagg cgtagagcct
    13501 acgacctcaa ggattacatt gatgttcttt ttgactggtt tcggttgaac tctgatgaat
    13561 ttacgctgtc aatagaatct gtggaaattg cagagttgtc acgcaatatt ttgaaagact
    13621 ggattcctat attccatgaa aagaaactga attttgaaat tgatattcca gaaaatcgac
    13681 taatggtaaa tctcgaccca gatggatatt cccgtgtggt taataatctg gttcaaaatg
    13741 ttcttgctca cagtaaggct cggcaaatca agattaccat gagtgaggat tccagaatgg
    13801 tattactccg tgttgatgat gatggtgttg ggattgccag agaaaattta ccacatattt
    13861 ttgagcgact gtataagtgt gataaaggac gttcagaaaa aggaagtggt cttggtctat
    13921 ctattgtcag tcaaatggtg gagcgaatgg gtggtcagat ttccgtagaa agtgagattg
    13981 gaaagtatac cgtatttaca gtgagcttac cattaatata aaattgtttt tcccccatgt
    14041 gacatgtata cacaggggga ttttttgtaa gttataaagt tgcaaggtta atacaaggtt
    14101 gttgcaaggt taatgtaaga ttgaggtggt atgatagctt taataaacaa aggaggattt
    14161 tattatttat gaataattta attattgaaa caaaaaatct cactaagcaa tatggtgctc
    14221 aaaagagcgt ttctaatttg aatattcacg ttcagaaagg tcgcatttac ggattgctag
    14281 gaagaaacgg tgcgggtaag acgacaacta tgaaaatgtt attgggtcta acaaaaccaa
    14341 ctactggaga aattaaaata tttggtcagg acattcacaa taacgagaag aagcttcttc
    14401 ctcgaattgg atgtttgatt gaatcaccag ggttttatcc taatctgaca ggaacagaaa
    14461 atctcaaaat ctttgcaagg cttagaggaa taccaaaaaa aaatgcagta gaatcagcac
    14521 tggatgtagt cggtttgcca aacaatgaca aaaagttatt ttctcaatac tctcttggta
    14581 tgaaacagag gttggcgatt gctcttgcca ttatgcatga cccacaactg ttgattttgg
    14641 acgagcctat caatggactt gatcctattg gtattgcaga agtaagaaag tttattcgtg
    14701 atctctgcac agaacgaggt aaaactattt tgatttccag tcacattctt tctgaaattg
    14761 ccttgttggc agatgacatt ggtattattg accatggtat tttgttggag gaagaaagtc
    14821 ttgcccaatt agaagaaaaa aacagtaaat acatacattt tgctgtatct gatgcagcac
    14881 atgcagcaag gcttctggaa acccaactta acctgaaaaa tttcaaagta gcagataatt
    14941 acaatattat gctatatgat acagatgttt ctgttgcaga aataaatcgt gctttcattc
    15001 taaacaacat tgaggtatca gaatctcatt tatgcgaaga tactcttgaa gattacttca
    15061 agaaagtaac agggggtgaa ggaattgctt gatataattt catgtgaatt ttcaaaactc
    15121 aagcggttaa aatttatact gatttctatt ttaggtgctt gcttatttcc aattcccgca
    15181 acaattttga ttgcaaagga caacttgccc tttgagcaat tgttcaaatt agttgtcaat
    15241 tttggatatt ttttgctatt accaattgta ttaagtattg ttgctagcca actatttttt
    15301 atagaaagag ataacgatgt attaaaaaat cttgcaacag tgccagtgcc aaaaggaaaa
    15361 ctggctctgg caaaattggc tgtgttgctg tttatatcat tgttctactc cgttgcaggg
    15421 ttaggagcta ctatcattgg aggtttcatt gttggaatag tagagggggt tactgtaaaa
    15481 ttaggaatga gcattgcttt tggaattatg ttttttgtta ctgtacttcc tgttgtggta
    15541 ctcattgtat atttcaatcg cagctatatt ttttctatta tactttcttt tgtatatgct
    15601 attttcaatt ttagtatatc cttaaatatt atcaattttg aaccaaataa cccactatta
    15661 agcgtattac ctgctcctgt tataatgcgt tggtggatgg cattttgggg agatcccact
    15721 ggcgaataca cggcacttag acagccatat ttgctctcaa ctcctgcttg tgtaggtatt
    15781 ttatgtttga taaccattat agcagtcctg ctgatttgta tgatatacaa aaagcaggag
    15841 aattaggagg tatctatgct tgatataatt actacagaat ttcaaaaaat aaaaagatat
    15901 aatattctat ggattggtgt tgttgctgtt ctgttttcag cactattggc agtttttcag
    15961 gttgtaagtt cacacggcag tgaccctcta acgtatgaga gctttgcaaa cggagttatc
    16021 tggaataatt tcagtcttag ttttccattt gggattacac ttattggagg atatcttatt
    16081 aaccgtgaat atacagacca aaccttaaaa aacatcctaa ctgttcctat ttcattacgc
    16141 aaattgctag tgggaaaatt aattgcggtt ggaggaattg ctgtattgtt aagtcttttc
    16201 agcttcttat gcacatttat tttggggttg ttattttgca atatcaatat atctgttaca
    16261 ctcattgtaa aatccttagt tcagattttg tctgttaatc tgtattgtta tgttgctgtt
    16321 ttgccgatta ttgcttattt tggtcgtaaa caaaatgcat ttctgacggg tgtagggatt
    16381 gcatttgtat acgggttttg tggtgtgttt gttgctggaa gaaatctgac agacttttat
    16441 ccaatcacag caggtcttgg tctagttgga tacaacaatg gtgctggtac tgtttatcag
    16501 ccacttatcg gtgctatgac tttgattgta attctaatct tgacaactat tctacttgta
    16561 tttacaccta attatgataa agttatgacc atatctaaga aaaaagggat aaagcataaa
    16621 aagtattctc ataaataaca taaatattgt ttctattatt taagttgcta gacagtgaaa
    16681 agaaaaaatc tgtcgtacag tggctatatg ctcatgtatt caagaagcta cagttttgat
    16741 ttttcataac tgtagcttct ttttgtattt attaaatcac ggctatcata atacttgcaa
    16801 taggtatagc gacaagagag agtactgtca tgtgtgtttt atatattaca ttaagtatat
    16861 tctaaaatat atgttttaaa taatatattt gaaaatagga atatttaaat aaaaaaaata
    16921 atattatata tagttgacaa aaattggcaa atgatataat atataaatat agaaatagta
    16981 atcattacta ttattaaaaa gggaggaatt aattatgaac ttaaaaggaa ctaaaacaga
    17041 aaagaattta atggcaggat ttgcaggaga gtcagaagca agaaataaat atacatacta
    17101 cgcttcaaaa gcaagaaaag aaggttataa tcaaatagct gctatatttg aagaaactgc
    17161 taacaatgaa aaggaacatg ctaaattatg gtttaaactt ttacatgatg gtatgccttc
    17221 aacagaggaa aatttaaagg atgcagcagc aggtgaaaac tatgaatgga ctgatatgta
    17281 tgcaaaattt gctaaagaag ctagagaaga aggatttgac aagatagctt atttatttga
    17341 agctgttgga aaaatagaga aagagcatga agaaagatac ttaaagcttt tagaaaactt
    17401 aaatgaagga aaaatattta aaagagatga agaagttgtt tggcaatgtc aaaactgtgg
    17461 tcatgtgtat gtaggtactg aagcaccaga aaaatgccca gtatgtgacc atccaaaagc
    17521 gtatttcaat ataaaagctg aaaattatta aaaattatca ttattaaaaa ggaatgttaa
    17581 ctatgaaatt ttctaaacaa cgagaactga ttttaaatga aatattaaat aatccggttc
    17641 atcctactgc ggattaccta tatgaaaact taaaaaaaga taatccaaat ttaagcttag
    17701 gaactgttta tagaaattta gctcagctaa cggaacatgg ctttataaga aaggttagta
    17761 ttccagggta tccagataga tttgatggtc gaatagataa tcactatcat atcatatgtg
    17821 aagtatgtgg agaggtatac gatttagaat ccgaagttct taataattta caagaattaa
    17881 tatctgagga gacagacata aaaataacat cttataacat aagttttaaa gggatttgta
    17941 ataattgtaa aaggtgtagc caagtaggtt agttttcaaa ttctaaaaat aattaatgag
    18001 gtgagtttat gtgtagtgaa caaaaatttt ttatatgtga gacttgtgga aaccttgtag
    18061 gaatgataca aagtggtggt gtacctattt tctgttgtgg aaaacctatg aaggaacttg
    18121 ttcctaatac aacagatgca gcagttgaaa aacatgtacc tgtaattgaa gtggatggaa
    18181 ataatgttac tgttaaagta agtagcacaa ctcatccaat gactaaagaa catcatatag
    18241 catgggtgta tcttatgact gaacaaggta gtcaacgcaa atgtctagca gtagatggag
    18301 aacctgtagt taaatttgct ttgaatgatg atgataaagt gatttctgcc tatgcttact
    18361 gtaatttaca tggattatgg aaggctgaac tgtaatattt aaataaattg agtcaaagag
    18421 gaggctataa atgtattttc atacaaatag cctcctataa atttatataa attcaagttt
    18481 taattttgtt taatgtcaat ataatataag gagaaaataa tatgtcaatt tataaatgta
    18541 gtgtttgtgg atatatttat gatgaatcta agaatgataa aacatgggat gaattaagtg
    18601 aagattggga atgccctgta tgtacaaaag gtcgtagtta ttttggaaag ataagcactg
    18661 tttactatga agaagatgaa aagatagcag aagacatagt tgaaaatgaa tctaaattaa
    18721 atactgaaaa agagggagat ttaaattact taagtacata tttaagaaga gatgatgaag
    18781 ttgagaaaca tatggatata attcacgaaa tggctgttac aggaaaatct ataatagaac
    18841 ctatgagaac taaactacca gtaatttctt gggatgatat tttaattatg ggtgctcaat
    18901 taaatccatt acctttaaat gagcatgatg aagtaaatac aacaacaata ataggaaaaa
    18961 aagcaaagaa acctatgatt atagaaaatc cagtttatat ttcccacatg tcatttggag
    19021 ctttgtctaa agaactaaaa atagctttag caaagggtgc agcccaaaat aagacagcta
    19081 tgtgtagtgg tgaaggaggt atattaccag aagagaagga agcttcatac aagtatattt
    19141 ttgagtatgt accaaataaa tatagtgtaa ctgaagaaaa tcttaaaaat tctgatgcta
    19201 tagagataaa aattggtcaa ggaacaaaac ctggtatggg aggtcatctt cctggcgaaa
    19261 aagtaactga agaaatagca aaagtaagaa ataagcctgt aggtcaagat gtaataagtc
    19321 catcttgttt tgaggaaata caatccaaag aggatttaaa gaaacttata gatgaactta
    19381 gagaagtttc agaagggcgt ccaataggtg ttaaaatttc agcaggtcat attgaaaaag
    19441 atatggaatt tatagcttat gctaaacctg attttgtaac tattgatggt cgtggtggag
    19501 ctactggagc aagtccaaag ttgcttaaag atgcaacttc aattccaaca atatttgctc
    19561 tttatcgtgc tagaaaatac atagatacac atggattaga tatagatttg gttataacag
    19621 gtgggcttcg tatatctact gattttgcta aggcaattgc tatgggggct gatgcagttg
    19681 ctattgcaag ttctgctctt atggcagcgg cgtgtcaaca atatcgtata tgtggttctg
    19741 gtaagtgtcc tgtaggagta gctactcagg atgaggaatt gagaaaaaga ttacatatag
    19801 aaaatagtgc taatagagtg gcaaactttt taaatgtaag tttagaagaa cttaagactt
    19861 ttgctagaat ttctggtcat aaagatatac atgatttaag tgttgatgat ttatatactg
    19921 ttaactctga aatatcaaat tatacaaaca tacagcatgt atagattgaa gtttgaaact
    19981 attagatttt agatagctat aaaagtaaat gataaatata ttttataaaa gagagtgtct
    20041 taaaatgaac tttttagttc ataagtcact cttttttgta tgaagccaaa tatatttatc
    20101 attttagcaa tgaaaaagaa tcttatctct ttattttgag atagactcct tctatttatg
    20161 aaaattaaag atattaaatt gtaaaatatt ctgtgatgtc gtactattaa actaaagatt
    20221 ctgtacttta aagtaaataa aaatttaagg agtgcttatt atggaaagat tgaagataca
    20281 agttttagtt gacaataata cttatattga tagatacttt gttggagaac cagcagtaag
    20341 ctattatatt gagattgatg gaaataggat tttgtttgat tcgggatatt cagatgtatt
    20401 catatcaaac gctgaaaaat taaatataga tttaggaaat ttaactcatg ttgttttttc
    20461 tcatggacat aatgaccata caagaggtat ccagttttta gaaaataggt atgatttatc
    20521 tacagttgaa cttatttcac atcctaactg ttttatacct agaaaacatg gagagaaaag
    20581 cataggagct ccattttctg ctgaggaaat taaaaatatt tttatgtata atcctaagga
    20641 taagccattt aatctaagta agaattgtgt gtttttaggt gaaatacctt ctataaataa
    20701 ttttgaaaaa agagctaaaa ttggtaaatg taaaatagga gatttgtggg aagatgatta
    20761 tgttttagat gattctgcta tagtttgtaa aactgataaa ggtattttta ttgttacagg
    20821 atgttctcat agtggaattt gtaatatagt agagtatgct aaaagggtat gtggagatga
    20881 tagagttatt ggtatactag gtggttttca tctttttgaa ttgaataata ggttagatag
    20941 tacaatacaa tatttggata aagaagaaat aagaatgttg tacccttgtc actgtgtttc
    21001 ttttaaggta aaagctaaga tgaatgaaat attgaatata aatgaagttg gagtagggct
    21061 gaccataagt atatagtatt tgaaagtata taagatttaa gttgaaggtt aaaaagatat
    21121 tttctatata aagtatcttt tttgttacgt ttattttaag agaaatcaat atacttttat
    21181 atgaaatatt gtaaaaaaat ttatttttaa tgagaatata ttttcattaa taaacaattt
    21241 tattatgtat taataatgct agaataaata taaataaatc aaagttactt acattaaagt
    21301 atattttttc acaaaatata ttgatttatt cttttaaagt ttgataattc caatgaatgt
    21361 aataaaagaa gggattttat actatacaat tattattata aaattataaa gaaaaaatta
    21421 aataataata aagaaaaaat gtataatatt taacattaaa aaagatattt cataaaatta
    21481 aaaaacaaag tgaatttgaa tactatttaa aaaataaaaa aatatataaa aaatatttaa
    21541 tacaaaggta gaaataagtt gaaaatgtaa agtcattgga aaaatatagt tgaatttaat
    21601 gaacaaaaat gttattgttt tatcaatgaa gtgttaaaat aaaaactttc aatatcattt
    21661 tagggaggat gatttcatgc aaaaaagtgt aaagaaagca aaagtaacgg gatctatgtt
    21721 agccattttt ggtgttgcaa gcgttctttt tagttcacat gcaggaggag gatttgctac
    21781 agggaatcaa gaaactcagt actatgtgca gtatggatgg acagcaccac ttatggcaat
    21841 tttagcaatg attatactta ctgctaccat gagagaagtt atcataatgt ataataataa
    21901 caattgtagg aattataagg atttattttg tgagctttgg agaccatatc caaaacttga
    21961 aataatatgg gagatatatt actatctaat ggttttaata gctgtaagtg ctgttatagc
    22021 aggtgcagca gcagtatttc aaagtatagg agtaaattat tttgtagctg tatttattat
    22081 tggagttgta cttcttgtct ttactatatt tggtgctatg cttgtatcta aagcagctac
    22141 agcaatgaca atagctattc tagtatgtac tcttactatt tttattgttg gtataaaagc
    22201 taaagtacca gaaataactg aaatattatc aaatagaacg agtttcacac ctggctatat
    22261 caagccaata cttaatactt ttatttatgc aggttttcaa tcggttgtta tacctacact
    22321 tgcaggatgt tcaagaccac ttcttaagaa ctcaaaagaa gctactaagg caatgataat
    22381 ttcatttgtt atgaatgcaa ttgcattagg acttgctgta actatgctaa tgggatggta
    22441 tagagaaatt attgctgctg ggcaaacaac actccctact ctttatgttg caggtcaatc
    22501 tggaaatcat actatataca taatttataa tgttgcatta ttcctttgtt taatgtctac
    22561 aggagtaact actatatttg gtcttgtaaa tagatttgaa gaccataaag cactttcttt
    22621 cctttcttca agaatgaaaa gaagggtatt tacagcttgt gcaataatgg tagtgtcaat
    22681 gcttatatca cttacaggat taagtaatat tgttaaatat ggatatggat attgtggtta
    22741 tcttggtcta ttcactatag taattccatt ccttacacta ggacattata agaataagaa
    22801 gtttgcaaag gaaaatccag aagctaaatg gccagcggaa ttaaatgaaa atgtaaatta
    22861 gatgtagtag caaaaaatat tgctaaagag aatactataa ataagaatac tatagataat
    22921 atatctgcat gtttattaag attatttgca aaatctaata ttgaacaact ataaacaagc
    22981 aatataaaac taagaataaa agataaaaag agggtgtctc aaaatggact tttagtttat
    23041 aaggcactct tttttatatg aagtcaaata tattttcatt attttgacaa taaaagagac
    23101 taatcctact ttggatagcc tcttttttct ttaatataaa atagtataac agcaaagtat
    23161 agattgatac atgttttctg tagctttcta tatattacag tgtttgtgtg gcattggtat
    23221 tttaagcaat ctataaatta taattctata attcttctac taattttaga ttctatagcc
    23281 gtctctattt cacttaacat tcgttcattt ttagggtcaa taaaagcaca tgtatagcct
    23341 tcttcaccag ctctaccagt tctaccaata cggtggatgt agtcttctgg tgtttctggt
    23401 aaatcgtaat tatatatatg acttactcca gatatatcaa ggccacgaga agctacatct
    23461 gtagctatta agtattgtat atctaaattt ctaaatgatt tcattattct ttctcgtttt
    23521 gattgagcaa tatcactatg aagtttttga caattatatc ctcttatagc aagagcttct
    23581 tcaagattgt caacccttct cttagtccta caaaatatta ttgccataaa aggattatct
    23641 tcatctagta ccttacataa ggcatctaat ttttttctat ctgtagtttc tactacttct
    23701 tgttttatat tattaagagt gatttcttct ttttgaatag aaactacaac aggatttttt
    23761 gtgtatctat aagctaattt ttttacttga gaatttatag ttgcagaaaa gcatagtgtt
    23821 tgctttttgt ttgaagtctc ttttaaaata gcttccactt cgtttttaaa tcccataagc
    23881 aacatttggt ctgcttcatc taaaacaaaa gtctttaact tactaaggtc tattgttttt
    23941 ctatttagat ggtctaaaag cctacctggt gtagctataa tcatatgaat atttcctttt
    24001 agtttcttta tttgagaacc aatatctttt cctccatatg ctgcaagaat acttacatct
    24061 ttagattctt taagtttcat agcttcttct gttatttgaa tagctagttc tcttgtagga
    24121 gaaagtataa gtacttgtat atcatttata tctaatgata tattttcaaa tatcggaagt
    24181 aaaaaagcta gtgtttttcc tgttccagtt tgagcttctg ctattacatc ttttccttct
    24241 tttattagtt taatactttc tttttgtata ggtgtaggtg ttgttattcc tgattttttt
    24301 agaatattta ttgtattttc actaatccct aagcttttga aatccattgt tatcttacct
    24361 ttcatgaaaa attttctttt ttattaacca attaataata tcatatttgt aagtattttt
    24421 agtaaatttt ttaattatta tttatatagc ttaaaaatat attaagatac tttaaaatgg
    24481 aatgtaactt aacttaaatt ttagtaggaa ttttataggt ttatttttaa aggttttttg
    24541 aaaataaatc tatttaaaaa ttaaattaaa atgcaagaaa aacataataa ctttcatttt
    24601 t
//
LOCUS       DAELSY010000050        24402 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.050, whole genome shotgun sequence.
ACCESSION   DAELSY010000050 DAELSY010000000
VERSION     DAELSY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.050"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            255..392
                     /gene="rpmH"
                     /locus_tag="KPG09_001759"
     CDS             255..392
                     /gene="rpmH"
                     /locus_tag="KPG09_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBG0966376.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            470..814
                     /gene="rnpA"
                     /locus_tag="KPG09_001760"
     CDS             470..814
                     /gene="rnpA"
                     /locus_tag="KPG09_001760"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBG0966377.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            846..1097
                     /gene="yidD"
                     /locus_tag="KPG09_001761"
     CDS             846..1097
                     /gene="yidD"
                     /locus_tag="KPG09_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBG0966378.1"
                     /translation="MNKIIYILKEISRYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            1129..1836
                     /locus_tag="KPG09_001762"
     CDS             1129..1836
                     /locus_tag="KPG09_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG0966379.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            1805..2434
                     /locus_tag="KPG09_001763"
     CDS             1805..2434
                     /locus_tag="KPG09_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBG0966380.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            2546..3925
                     /gene="mnmE"
                     /locus_tag="KPG09_001764"
     CDS             2546..3925
                     /gene="mnmE"
                     /locus_tag="KPG09_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBG0966381.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGERALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNILDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKEIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            4020..5915
                     /gene="mnmG"
                     /locus_tag="KPG09_001765"
     CDS             4020..5915
                     /gene="mnmG"
                     /locus_tag="KPG09_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBG0966382.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVDKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQVRR
                     TRGGKGE"
     gene            5908..6627
                     /gene="rsmG"
                     /locus_tag="KPG09_001766"
     CDS             5908..6627
                     /gene="rsmG"
                     /locus_tag="KPG09_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBG0966383.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGRPAKNPIK"
     gene            6802..7587
                     /gene="noc"
                     /locus_tag="KPG09_001767"
     CDS             6802..7587
                     /gene="noc"
                     /locus_tag="KPG09_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBG0966384.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            7722..8495
                     /locus_tag="KPG09_001768"
     CDS             7722..8495
                     /locus_tag="KPG09_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBG0966385.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            8497..9360
                     /locus_tag="KPG09_001769"
     CDS             8497..9360
                     /locus_tag="KPG09_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBG0966386.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            9393..10535
                     /locus_tag="KPG09_001770"
     CDS             9393..10535
                     /locus_tag="KPG09_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG0966387.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVSDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(10669..11244)
                     /locus_tag="KPG09_001771"
     CDS             complement(10669..11244)
                     /locus_tag="KPG09_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBG0966388.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDTSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            11504..12796
                     /locus_tag="KPG09_001772"
     CDS             11504..12796
                     /locus_tag="KPG09_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0966389.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            12901..13503
                     /gene="yedF"
                     /locus_tag="KPG09_001773"
     CDS             12901..13503
                     /gene="yedF"
                     /locus_tag="KPG09_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBG0966390.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            13576..13839
                     /locus_tag="KPG09_001774"
     CDS             13576..13839
                     /locus_tag="KPG09_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBG0966391.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            13854..14039
                     /locus_tag="KPG09_001775"
     CDS             13854..14039
                     /locus_tag="KPG09_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBG0966392.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            14142..15338
                     /locus_tag="KPG09_001776"
     CDS             14142..15338
                     /locus_tag="KPG09_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0966393.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLEKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            15552..15830
                     /locus_tag="KPG09_001777"
     CDS             15552..15830
                     /locus_tag="KPG09_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBG0966394.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            15867..16301
                     /gene="ssb"
                     /locus_tag="KPG09_001778"
     CDS             15867..16301
                     /gene="ssb"
                     /locus_tag="KPG09_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG0966395.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            16320..16547
                     /locus_tag="KPG09_001779"
     CDS             16320..16547
                     /locus_tag="KPG09_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBG0966396.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            16690..17010
                     /locus_tag="KPG09_001780"
     CDS             16690..17010
                     /locus_tag="KPG09_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG0966397.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            17018..17974
                     /locus_tag="KPG09_001781"
     CDS             17018..17974
                     /locus_tag="KPG09_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBG0966398.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            17985..19979
                     /locus_tag="KPG09_001782"
     CDS             17985..19979
                     /locus_tag="KPG09_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBG0966399.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            19980..20429
                     /locus_tag="KPG09_001783"
     CDS             19980..20429
                     /locus_tag="KPG09_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBG0966400.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            20448..21776
                     /gene="dnaB"
                     /locus_tag="KPG09_001784"
     CDS             20448..21776
                     /gene="dnaB"
                     /locus_tag="KPG09_001784"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBG0966401.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            21981..22289
                     /locus_tag="KPG09_001785"
     CDS             21981..22289
                     /locus_tag="KPG09_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966402.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            22371..23660
                     /locus_tag="KPG09_001786"
     CDS             22371..23660
                     /locus_tag="KPG09_001786"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBG0966403.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNKIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            23986..24061
                     /locus_tag="KPG09_001787"
     tRNA            23986..24061
                     /locus_tag="KPG09_001787"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24019..24021,aa:Lys,seq:ttt)
     gene            24068..24142
                     /locus_tag="KPG09_001788"
     tRNA            24068..24142
                     /locus_tag="KPG09_001788"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24101..24103,aa:Glu,seq:ttc)
     gene            24147..24222
                     /locus_tag="KPG09_001789"
     tRNA            24147..24222
                     /locus_tag="KPG09_001789"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24180..24182,aa:Val,seq:tac)
     gene            24256..24330
                     /locus_tag="KPG09_001790"
     tRNA            24256..24330
                     /locus_tag="KPG09_001790"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24288..24290,aa:Thr,seq:tgt)
BASE COUNT         9975 a         2574 c         4503 g         7350 t
ORIGIN      
        1 acatgttaaa attactttat ttgtgtatat aattgaaaaa aataatttag aataaaatta
       61 aatagatttt tgctagtatt ttcaataaaa aaaatacata atatatatat ggtagatttt
      121 gtgatataag cgtcccaaaa actcgttgac aacagtagta aaagtattta taattatatg
      181 tgttatgttt actttgaggg taaagttatt atttttataa acggaaaata aaatctgaaa
      241 ggcggtgcaa aataatgagt aaaagaactt atcaaccaaa aaagagacaa agaagtaaag
      301 aacatggttt cagaaaaaga atgaagacgt ctaacggaag aaacgtgtta aagagaagaa
      361 gagctaaagg aagaaataga ttaactcatt aattacaaaa gggccgctgc aaaggtggcc
      421 tttttgtgta aataattaca attcatagct gtgtttatgg aggttaacta tggactttaa
      481 taggactaaa gggttgaaaa aagactctga ttttagaaaa gtatataaac acggcaaatc
      541 ttttgcaaac aaatatttag taatatatat acttaaaaat aaatcagatt atagtagagt
      601 aggtatttct gtttcaaaaa aagtaggaaa agctattact aggaatagag taagaagatt
      661 aataaaagaa gcctacagat taaatattga tgaaaaaatt aagcctggat atgatatagt
      721 atttatagca agggtatcta gtaaggatgc tacttttaaa gacatagaca agtctataaa
      781 aaatttagtt aaaagaacgg atatttctat ttaattgtat gctataaata tggaggttaa
      841 tgactttgaa taaaataatt tatatattaa aggaaataag taggtattta tcgaatttat
      901 gtatttattt agtgagattt tatcaaaagt atatatctcc cttaaaggga cctacctgta
      961 gattttatcc aacttgctca caatattcaa tagaggcgtt taaaaaatat ggatttgtaa
     1021 aaggtatgta tttaacgata agaagacttt taaaatgtca tccttttcat ccaggaggtt
     1081 acgaccctct aaaataaaag agagtaacta ttaggaggta taaaaaaatt gaatataata
     1141 ggaaatttct taggattgat tcttaaggta atatttgaat ttgttaatca ttatggtatt
     1201 tctataatac tttttacgat acttgtaaag attattttat taccattaac aattaagcag
     1261 actaagtcaa caaaggcaat gcaagatata caacctcgta taaaagagat acaagaaaaa
     1321 tataaaaata agccagaaaa acaaaatgaa gaaattgtta agttatatgg agaagctaaa
     1381 attaatccac tatctggttg tttaccttta ttaatacagt ttcctatatt aataggatta
     1441 tttagcgttt tgagagaacc agttgctcat ggcgtttttg caaacaaagc tgcattttta
     1501 gcagctgaca atgggttttt atggataaaa agcttaacat caccagatta tgtattagca
     1561 gtattttcag gagcaagtgc atatgtcatg caaaaagtaa tgacacccaa agatcagtta
     1621 caaggctcta tgaaagtaat gacatatgta atggcaggta tgtctttcta ttggggattt
     1681 atattcccag caggacttac attgtactgg acagtaagta acttattctc aatagctcaa
     1741 tattacttaa taatgaatcc tttaaaagct aaactggctg ctaactctaa ggaggaaaaa
     1801 gttaatgaga aaaagcctaa aattaataaa aagtaagagt aaagaggaag ctataagtaa
     1861 agctatagca gagttgaatt taaaagcaga ggatatagaa gtagaaatac ttgaaaatcc
     1921 aagtaaaggg tttttaggtt ttataggtgc taaagatggt acttatgaaa tttttgttat
     1981 cgagagggaa gtagatgtag ctaaaaattt tatagaggta atgttgaaaa atgcaaatgt
     2041 tgatgccaaa gtcaatgtat ctcaaaaaga taatttaatc atggttgata ttgaaggcaa
     2101 agaagctgca agtttaattg gtagaagagg ggaaacttta gattctattc aatttttgac
     2161 tggattagct cttaataaaa taaataaaga ttctcataca agagtacttg tagatataga
     2221 aaattataga tcaaaacgag aagaatcttt aataagatat gctaataaag ttgctagaga
     2281 agtagcgaaa acaagaaaaa ctaaaaaatt agattatatg aatccatatg agagaagaat
     2341 aatacattca gctcttcaaa atgacaaata tgtaattaca tatagtgaag gaaccgatcc
     2401 atatagaaga ttagtaattg agtgtaaaag ataaagtcaa cctcctaaac attagggggt
     2461 tttatttttt aatattatta aatcattaat gattggttaa atgtaaacct atatgtaaaa
     2521 aattatgaat taagaggtga taaatttgtt tatagatgat acaattgcag caatagctac
     2581 agcacctgga gaaggtggta taggtatact tagaataagt ggagaaagag ctttgaaggt
     2641 tgcagaagaa atctttaaat ctatgtcggg aaagagtatt gaagaataca ataagagaac
     2701 attaatatat ggaaatatat tggataatga aaatataatt gatgaagttt tattggcata
     2761 tatgaaagga ccaaactcct atacaggaga agatgttata gaaattaatt gtcatggtgg
     2821 atttatttcg gtaaagaaga tattagaatt aatactatca aaagatgtta gattagcaga
     2881 ggctggtgag tttactaaaa gagcattctt aaatggaaga atagatttat ctcaagcaga
     2941 ggctgtaatt gatgttataa aagccaaaac tgatatagct catgaagttg ctcaaaatca
     3001 attagagggt tctttatcaa agaagataag agaattgaga gataaagtca ctgagatatt
     3061 agctcatgta gaagttgcta tagattatcc tgaggaagat attgagcata taacatatca
     3121 aactcttaaa gaaaaaactg atgaacttaa aaaagagatt aaaaaattat atgatacagc
     3181 tgaaagtgga aaaatactta gagaaggctt aaagacggta attgttggga aaccaaatgt
     3241 aggtaaatct tcactactta attctatttt aggagagaat agagcaatag ttactgatat
     3301 accaggaact actagagatg ttatagaaga gtttgtaaac ataaaaggta tacctttaaa
     3361 aattgttgac acagcaggta taagggatac tgatgatatc gttgaaaaaa taggtgttga
     3421 aaagtctaaa gaatcattta ctagtgcaga tttgatagta atggttttag atgcatctag
     3481 aaagttgtca gaggaagata tagagatatt agaaaaactt aaggataaac aaacaatagt
     3541 gctgttaaat aagaatgatt tgaaacaaga gattgaagaa gaaaaaatac ttaaatatgt
     3601 agaaaataat agtataataa aaatatcagc tttacaacaa gaaggaatag aagaattgca
     3661 agataaaata gagtctatgg tatataaagg tagtatcaaa aataattcta gtttagtagt
     3721 aacaaattca agacataaag atgcacttag taaagcatat aaatctgcaa cagatgcttt
     3781 aattgctttg gaacaaagta tgccatttga ctttgtagaa gtcgatttaa aaaatatatg
     3841 ggactatctt ggatatatta atggtgatac agtaacagag gatttactag ataatatatt
     3901 ccataatttc tgtattggta agtaatgtac aaaagtaaat ttgtttttat tatagagatt
     3961 tttaaaaaat ttgaagtgtt tctcatgaaa cattaatttt gaaaagaaag gaattataaa
     4021 tgataaagtt tgaagcagga aaatatgatg ttatagtagt cggagcagga catgctggat
     4081 gtgaagctgc tttggctact gcaagaatgg gatataaaac tttgataata acaatgtctt
     4141 tggattccat agcattaatg ccttgtaatc catcaattgg agggactggt aaaggtcaat
     4201 tagttaaaga aatagatgcc ttaggtggtc agatgggatt aaatatagac aagacatata
     4261 tacaaagtag aatgttaaat actgccaaag gtccagcggt acactcttta agagcacaag
     4321 cagataaatt taaatatcat gaagaaatga aaaagacatt agaagatgaa ccaaatcttg
     4381 atatagctat ggatgaagtt gtagaaatac ttcatgaagg aaatgttgta ataggagttg
     4441 gaactaaact aggatgttca tttaaatcta aagctgtaat attggccact ggagtttatc
     4501 tgaactcaaa gatatatatg ggtgaagttg cattttatga aggtcctaat gcattggggt
     4561 atgcaaaata tcttacagat agcttagttg agcttggtct tagaatgaga agatttaaga
     4621 cgggaactcc agcaagagtt catagagata gtatagactt ctctgtaatg tctttacaag
     4681 agggcgatga aaaagtaact ccattctcat ttatgaatga aaatatagaa aaaaaacaag
     4741 aaccttgtta tttaacaaga actacagaag aaactcaaaa agttatactg gataatttaa
     4801 aaaggtctgc tatgtatagt ggagtaatag aaagtactgg tccaagatac tgtccttcta
     4861 tagaagataa agtagtaaga tttagtgata aaacctcaca tcaattattt atagaaccag
     4921 aagggttaaa tactaaagaa atgtatatac aaggaatttc aactagttta ccttttgaag
     4981 ttcaattaga tatgtacaaa actataaagg gacttgaaaa ttgtaagata atgagacctg
     5041 catatgctat tgaatatgat tgtgttgacc caactcaact aaaaataagt ttggaaataa
     5101 agggtgtcga gaacttattt agtgcagggc aatttaatgg aacttctgga tatgaggaag
     5161 cagcagctca agggcttatg gctggtataa atgcagttag aaagatagaa ggaaaagagc
     5221 cgtttgtatt agaccgttca gaagcttata taggtgtctt attagatgac cttgttacta
     5281 aaggaactaa cgagccttac agaatgatga catcaagagc agaatatcga ctatatttaa
     5341 gacaagataa tgcagatatg agattaactc aaaaaggtta tgatatagga ttagttaaaa
     5401 aagatagata tgaaaggttc ctaaataaaa aggctgctgt agataaagaa tttgaaagat
     5461 taaaaaatga aagagttaca ccaaaagaag taaatagttt gttagaagaa aaaggagcaa
     5521 caccaataaa ggttggaata tctctatatg aatttttaaa gagaccagag gttacttacg
     5581 aattgcttga agaattaggg aagggcgctg gagaagatgt ttcaagagaa gttaaagaac
     5641 agtgtgtaat aataactaaa tatgaaggtt atatagagaa acaattgaaa caaatagacc
     5701 aatttaaaaa acttgagaat aaaaagctgg atgagaaaat aaattattca tctatagaag
     5761 gtcttagatt agaggctaga cagaaattag atgatataaa acctatttca atagggcaag
     5821 catctcgtat atcgggtgtt tctccagctg atatatcagt tttacttata tacctagaac
     5881 aagtcagaag aactagaggt ggcaaaggtg aataatatag aacttctgaa aaacgggatt
     5941 gaaggtttta atatagatac gaatgacagt atgttagaaa aatttaaaat ctatagagag
     6001 atattagttg aatggaataa aaagatgaat cttactggaa tagaagatga aaaagaagtg
     6061 tatataaaac attttttaga ctctatttca gctgtaaaga atggatatat aaaaaatggg
     6121 atgtctataa tagatgttgg tacaggtgct ggttttcctg gaataccatt gaaaatatgt
     6181 ttagatagct tagaacttac tttgttagat tcgttaaata aaagaataaa ctttttagag
     6241 gaagttagta gagtactaga gttagataat attacattta tacatggaag agctgaagat
     6301 tttggtaaaa atgaagacta tagagaaaaa tatgatgttg ctacggcaag agctgttgct
     6361 ggattaccga ttcttatgga attttgtgta ccatttgtaa aagttggagg gtattttatt
     6421 tgcttaaaag gtccgaatgc taatttagag ctagaagaat cacaaaaggc tattgatgtt
     6481 ttaggattgg agtatattga aaaaatagat gtagagttac cagaggtaga tttaaatcat
     6541 aatatattag tttttaaaaa ggtgatggaa acaccagtta agtaccctag aaaagctggt
     6601 agaccagcta aaaatcctat taagtaatga aaaaagtaat gaaaaaatta ttattggtaa
     6661 gcttaattat ttcttaggaa ataattaagc ttaccgtaaa aaaacaaaaa taaaggaatt
     6721 attaaagtag atgcagaaat aaatttgtat gtacatatag agaacaaaaa taatttatat
     6781 ctaaaataag agaggtatgt tatggaagat aaaagagtta tggagatacc tatagaagat
     6841 gtagttccaa atccatatca accaagaaaa atattttcac aagtttcatt ggaggaattg
     6901 agcaattcta taaaggtata tggtataatt caacctataa cagttagagc aaaagatggc
     6961 aaatatgaac taattgcagg agaaagaaga ttaagagcag ctaaattagc agagcttaaa
     7021 accataccag caattataaa taatatgaat gatgaatctt ctgctgttct agcgttatta
     7081 gaaaatttgc aacgagaaga cttaaatttc attgaagaag caattggtta tgaaaactta
     7141 ataaaagaac atgcttttac tcaacagcaa ttagcagaaa aattgggcaa aaatcaatct
     7201 actgtagcaa ataagcttag aatattaaag cttccaaacg atataaaaat gaagctaata
     7261 gagaataatt taacagaaag acatgctaga gcatttctta aactaccaag tgaagattta
     7321 atgcaaagtg tgttagacaa agtcataaaa aatgaattga ctgttaaaaa aacagaaaaa
     7381 ttgatacaag atgtattaga agaaactaaa gttcaagaag agcctgataa aaaacagaat
     7441 ataaagggtg ctatgagtat aagaatttac ataaatacta taaagcaggc ctttgatgct
     7501 atatcaaata caggaattga tgctaagtat aatgaaatag ataagggaga ttatatggaa
     7561 gttgttgtga aaatccctaa aaaatagaga ttactgctta ccagtaatct ttttttgtaa
     7621 tataagcctt gtaatataaa ttgatttata gacattattt atacttaaaa aacacctatt
     7681 caacatttaa aattaattta attcttaaag gggggaacat catgggcaaa gttatagctg
     7741 tattcaatca aaagggtgga gtcgggaaaa ctacaacaaa tgtaaattta agtgctagct
     7801 taggaacttt gggaaagaaa atattagtat tagatttaga cccacaagga aatacaacta
     7861 gtggatatgg tattaataaa aacgaagtag aaaatacaat ctatgaaatt atgttagatg
     7921 ggttacatat taaagaagca ataataagta ctgaatttga gaatgtagat gtagttccat
     7981 cagctactga actatcaggt gcagagatag agcttacatc taaaactaat agagagtata
     8041 ttttaaaaaa ttccataaaa gcggtaatag atgaatatga ttatatattt ttagattgtc
     8101 caccctcact ggggatgctt acaataaatt gtttaactgc tgtagatagt gttttaattc
     8161 caatacaatg tgaatattat gctttagaag gtgttagtca attaatggag actataaaat
     8221 tagtaaaatc aagattaaat gctgatatag aaattcaagg tgttgtttta agtatgtttg
     8281 atggaagagc taatttatca atacaagtag ttgaagaagt taagaaatac tttaaaggta
     8341 gtgtatatac aactttaatt ccaagaaatg taagacttgc agaagctcca agtcatggaa
     8401 aacctgtaat ttattatgat aaaagatgta gagggtctgt agcttatttg gagctagcag
     8461 aagaatttat agatttagaa gaggaggaat ggtagtatgg agaataagtc taaaagaagt
     8521 aatagattag gtagaggtct tagtgcattg ataccagaaa ttaaaggaga aacttcagaa
     8581 aaagaaatag tcaacataga tatagacaaa atatatccaa acgaagttca acctagaaag
     8641 caatttgatg aagaaaagat aaaagtattg tcggactcta taaaaaatta tggtgtttta
     8701 cagccaatag tggttaaaat ggatgaaaat aataaatata tgataatagc tggagaaaga
     8761 agatttagag catcaaaact tgcaaataaa aatcaaatac ctgctataat taaagatata
     8821 gatatgaaag atataatgga gatagcctta attgaaaact tacagaggga agatttaaat
     8881 tcaatagaag aggctctagc atataagagt ttaatagagc attacaatgt aactcaggaa
     8941 gaaatatcag aagctgttgg gaagagtaga cctcatataa ctaatacact aagactttta
     9001 aatcttggac aagatgttat agaaatgata gacagtggta gaattacagc aggccatggt
     9061 aaggctttat tgagaattgc agataaagat ttgcaacttc aaatagcgaa aaagatagaa
     9121 gaagaagaac tttctgtaag agaagtcgaa aatatagcta aaaaaatatc tgaaaataaa
     9181 caagaagaac ctaaaaaatc taaacctaag gatgtattta ttttagatgt tgaagataaa
     9241 cttagaaata tatttggaac aaaggtgaat atttctaaag gaaagaagaa aggtaaaata
     9301 gagatagaat actataatga tgatgattta aatagtattg tatcaatgct tcttgaatag
     9361 aacaaaaata taaaacagag aggaagattg ttatgattta tttagataat gctgcaacta
     9421 catatccaaa accagaaaga gtgtataatg ctgttttaga ttgtatgaaa aattattgtg
     9481 caaatccagg tagagcaggc cataaacttg ctatgagagc tgctagggag atatatgata
     9541 caagagagaa tatagctaag ttgttcaatg taagtaatcc tatgaatata gtatttacat
     9601 ctaatgcaac agattcttta aatttagcga taaaaggtgt attacaagaa ggagaccatg
     9661 taataacaac gtctatggag cataattcag taataagacc tataaaagca ttggaaaaaa
     9721 ggggtattga aaatactgta gtaaaatgcg attatgaggg atttttagat tatgaagatt
     9781 tagaaaaatc aataaagagt aatactaaac tgattgtaac aacacacgca tctaatgtat
     9841 gtggaacttt aatagatata aaaaaagttg gagaaatagc taaaaaacat aatatactat
     9901 ttttagtaga tgcatcacaa acagcaggtg tttatgatat agacgtaaat gaatgtaata
     9961 tagatatgtt ggcaatgcca ggtcataaat gcttatttgg gcctcaagga acaggtatat
    10021 tatatgtaag agaagggttg aacttaaata tattaaaaga aggtggaaca ggtagtaaat
    10081 cagaagaaat agttcaacca gagttattcc cagataagta tgaatcagga actcataata
    10141 ctccaggtat agcaggtctt aaccaaggta tactatttat atttgaaaga ggaatcaaca
    10201 atataagaca acatgaagaa gaattatgtc aatatatgat agacaaacta gaggaagtat
    10261 cagatataaa gatatatgga ccaaaggata gtaaaaaaag agcatcagta atcgctctaa
    10321 atataggaga tatggattct ggagaggtta ctttcttatt agatagtgat tataatatag
    10381 caactagatc aggaatacat tgttcaccac tagcacatac cactttaggt actttaaaac
    10441 aaggtgcagt aaggttcagt ataggatatt tcaacacaaa agatgagata gataaagcag
    10501 tggaagcttt gaaaaaaatt tcaaaaaata aatagcatga gatttaataa tatagaaata
    10561 aaaaaagggg atatttcaaa ataaaaattt gaatcatctc cttttttgta caaaaaagaa
    10621 aaggggctcc taagaacctc taatctattt gataattgtt tcaagaaatt actttaagta
    10681 atctccttct tcaagaacta tatgaccact ttcgtaatta gaaccatcaa cagtaatttt
    10741 tatttgttca acattcatat tttttatgta agtacgagca actgaatcaa gcattaaagt
    10801 ttctgcatca ctacctaagt ttgactttgt aaagtcagag ctaacatcta atataccagt
    10861 ttttacacca tcaatgtctt ttgtagtaaa tgaatttaat tttgcagatt ctggaacaac
    10921 tttaagtttt tgaagttctt caaataaagt gttctcatca attttattta aatcaacttt
    10981 attttcaatt aaatctgtct tatctacatc aaaagagtaa atagtagctg attgagtttt
    11041 agtttcttct tttggagtag gtttagcttt gttattagga acctcttttt cagtattgtt
    11101 ttcttctttt ggttgttcaa catttgtatt gctcttagtt tcttttttag atgtatcact
    11161 tccattttga gcattagtac aaccaactgc gaatatagat atagataata tacttaacac
    11221 taacattatt ttcttatttt tcataaaaat ttctcctctc ttaaaaacta ttcttatata
    11281 taattatagt cgttatgagc aaatatatga atacaaaata gtaacaaatt taaaaaatga
    11341 tttatttaaa ttaataaaac tcaaaataaa ctaaatttta gtcattttta tgggaaatca
    11401 tttaatgtac agtgaaaata ttgtaaaata aaagtagtgt ttttaaaata aatataagtt
    11461 ataattatat tataaaaaaa tgtcttcaaa aggtgggaat agtatggaaa ttttgagctt
    11521 aggagaaaag attaaaaaat taagaaaaga aaaaaacatg acattaaaag aattagctgg
    11581 agataggata acagcagcac aaataagcca tatagagagg gataaatctc atactagtta
    11641 tgaactgttg gaatatctag gcgataagct agatgtaagt gtagaatact tattagagac
    11701 caaggaaatg caatcaaaaa agataactga taatttaata ctacagagcg aaatttatat
    11761 aaaagaaaat gaattaaaca aggctgagga tcaaataaaa gaagttttag atatatgtaa
    11821 gagatacgat ttaatggaga actatggcag atgtaatttt ttaatggcta atattaattt
    11881 gaaaaaatct aattataatg atgctataga aaactttgaa aaggcattgt acttctttat
    11941 aaaaaatagt gatagtgaaa atatacttaa gtgttattta aacataggga aaatatatat
    12001 gcaggaagac ttttataaag gtgctataag ccactttaat tttgcagagg aaattttgtc
    12061 agagaataaa tttgaggatg ttaacataca caaggaacta tatagtaaga tggcatattg
    12121 ttacataaaa ctagataatc caaatatgtc attgaagtac atagaaaaga taaatgaaat
    12181 agataataga aatgatagaa aagaagatgt cgatattttg gtgttaaaag caaataatat
    12241 gcttgagatt ggaaaatatg aagaatcaag agattacttt aaaaaagcac taaaaatact
    12301 tgagagtgag gataataaaa ctggattagc aaatgtgtat ttaacaattt gtgatgttta
    12361 cagaaaaata ggtgaaacag atagagtact tgaatattca caaaaggtat atgacataaa
    12421 gaaaaatgat gaagatgaat acatgatgtc aggattattt aagataatag aagcatatat
    12481 agataaagaa gattatgatt tagctagaaa atattgtaag atagccttag cttcatcaat
    12541 aaaaaataaa aataaattca atgaatataa agcattgaga ttctatgcta atatgtataa
    12601 aaatcagaat gaagtaactc ttgcaattga atatctgatt aaatgcataa aaatagtatc
    12661 agatttaggc aacaatagaa tattggccaa tttatatata gacttaggtc aattatattc
    12721 aaatatatca aaagaaaaag agttagaata ttatcaaaaa ggtgtattta tgtacaaaaa
    12781 tttagaaata atgtagggta aataaaccta taagggtata gaaacaatct ataacaagca
    12841 agataatatc tatgataaaa tataaattgt atgaatattt ttaaggagat gaatcagaca
    12901 atgagtataa aaatagatgc gagagggctt gcttgtccta aaccagttat aaatacaaaa
    12961 agagaattgg ataacctaga agaaggtgtt gtagttacaa tagttgataa tgaaacagct
    13021 aaggagaata tattaaaact tgctaaatct ttaagttgtg aagcaaatat tgttgacgaa
    13081 aaagagaatt ttatatctat agaaattaga aaaggtaaaa atatactaga taaacaaaat
    13141 gttgtagata ataaagaaag tgaattagac aatacatgta tatttatatc ttcagataag
    13201 atgggacttg gaaatgacga attaggaaaa gtgttaataa aaggatttat atatacatta
    13261 acagaatcta aaccttatcc taagtatgta cttttagtta atggaggagt aaaattaagt
    13321 gctgaaaatg aagaaaccat agaaaatcta aaaatacttg agaatatggg tgtagaagtt
    13381 ttatcttgtg gtacttgttt agattattat aatttaaaag acaaattaca agtaggtaca
    13441 gttacaaata tgtatactat agttgagaca ttaaagaatg catcaaatac catttctata
    13501 taatgtatgt ttgtttaata atataaaact aataataaag attaactcaa atagatagtc
    13561 agaaaggatt aagtaatgaa tcaaatgtat atagtgtcat ttaattcgac acatcatgcg
    13621 ataagatcag aaaagttatt tggtgaaaat agtctaaaag taatggctct tccaacacca
    13681 agagaaataa cagcaagttg tggaatatca attaaatttt cttttgaaga tatggaaaag
    13741 ataaaaacta ttttggttga aaataatgta gatataaaag gaatttattg tataagtaaa
    13801 ttagaaaatg gttcaaaaga agtagagaag ttagattagg aggctaatta tttatgccaa
    13861 tggatttaaa aataggtgat attgttgaat taaaaaagca acatgcatgt gggtgtaaag
    13921 agtttgaaat cataagaacg ggaatggata taaagataaa atgtactaaa tgttcaaggc
    13981 taataatgct tgatagagag accttagaaa agagagtaaa aaagctaata aaaaagtgat
    14041 aaaaatttaa cacttaaaaa atgtcaaata ccgtatatta atatccaaaa ttttggtata
    14101 ataagaattg aagaaaacat tttcatgggg gtgctttaat tatggcagtt aaatatgcaa
    14161 aaagaatgca aggattacaa ggatcagaaa tacgtgagct tttaaaactt actcaacaac
    14221 cacaaataat atcttttgct ggtggaatgc cggctccaga gttatttcca gttgaggaaa
    14281 tgaaaaaagt atcagttgca gtgcttgaag aaaatggaag atcagctatg caatatacta
    14341 caacagaagg atatgaacca ttaagagaaa aaatagcagc tagaatgaat gataaaaaca
    14401 aaactaatgt aaacaaagat gatatattag ttacaagtgg ttctcaacaa ggtctagatt
    14461 ttgcaggaaa agtatttatt gatgagggtg atgtaatttt atgtgaaagt ccatcttaca
    14521 taggagctat aaacgcattt aaatcttatc aacctaaatt catagatgtt ccaacagatt
    14581 ctgatggaat gataatggaa gaattagaaa agatactaga aacaactgat agaataaaaa
    14641 tgatttacgt aatacctgac ttccaaaatc caacaggaag aacttggcca cttgaaagac
    14701 gtaaaaaatt catggaaata gtaaataaat ttgaaatacc agtaatagaa gataatccat
    14761 atggagattt aagatttgaa ggagagactt taccatcttt aaaatcaatg gatacaaaag
    14821 gattagtaat attcttagga actttctcta aaatattctg cccaggatac agattaggat
    14881 ggacttgtgc ttctccagaa attttatcta aatttaactt tgcaaaacaa ggtgcagact
    14941 tacaagcatc aactatatct caaatggaag taagcaaatt tatggatatg tatgacctag
    15001 atgcacatgt tgacaaaata aaagcagttt atgttaaacg tagagatgta atgcttaaga
    15061 ctatggaaga agagttccca gaaggattag tatttactca tccagaaggt ggattattta
    15121 catgggtaga gcttccaagt aatttaaatg ctaaagaatt aatgccaaaa tgtttagaga
    15181 agaatgttgc ttatgttgct ggaggaggat tcttccctaa tggtggtaga gaaaatactt
    15241 tcagacttaa ctattcaaat atgccagaag aaaaaataat agaaggtata aaaaatatag
    15301 cagctgtatt aaaagaagct atgggtgtag aagcttaatt aaaaataata acttatatat
    15361 aatacaaggg atggttgttg ccatcccttg tttgtttgaa aagtatataa ttcaccttat
    15421 actaaacttt gtccaaaatt taaattgaca gaaataagaa taaatgataa aatgtgtagg
    15481 tatgagattt tagtctcatt tctctgctct aagagtgtag agccgttaag tccaaaggga
    15541 ggtgaattat agtgagaaat tatgaattag tttatgtggt aaagccaaat tctgatgaag
    15601 aagtaagaga ggctatacta aacaaagtta aggaagttgt tgcaactgac ggagaaatag
    15661 ttaaagttga tacttgggga actaaaaaat tagcttatcc aatagctaag tttacagaag
    15721 gtttctatgt attagttaac ttcaaatcag cagtagacgt tcctaaagag atagacagaa
    15781 acttaaagat aaatgaaaac gtaataagac acatgatagt tgttgcttaa taactggttt
    15841 tattataaaa aataggtggt gtttttatga atcaagttgt attagtggga aggttgacta
    15901 gagaccctga attaagatac atcccaggta caggaacacc agtggcatcc tttacaatag
    15961 ccattgatag agactatgta aaaaaagatg gatctaaaga aactgatttt atacctgttg
    16021 aagtaatggg gaaatctgct gaattttgtg ctaactacat aactaaaggt agattagtag
    16081 cacttcaagg atctataaga gtagacaatt atcagacaca atcaggtgaa aagagaactt
    16141 ttacaaaagt aagtactaga tcagtacaag ctttggatag taaaaataaa tcggaaaatt
    16201 catatagaga gagtgctcca gcttttgagc caagctttga acctcaaggt ttagacccac
    16261 aaggttttca agctatagat gatgacgata taccatttta aaaaggaggg aaaaaagtca
    16321 tgatgaataa gaaaagacgt aagaaaaaaa gagtttgtca attttgtgct gacaaaaacg
    16381 ctaaaataga ttacaaaagt actcaaagat tacaaaagta tataactgaa agaggaaaga
    16441 tattaccaag aagaatatct ggaacttgtg ctaagcatca aagagaatta acagttgcta
    16501 taaaaagagc gagaaatata gcacttttac catatacatt agactaaaaa aatgagcctt
    16561 gtgctcattt tttattatat gtatttatat atctaaatga ggcgagctat aaagctcgtt
    16621 tttttgttta tatacttaaa tatattatta tttatgatat aatattatat attaaggagg
    16681 gttgtaaacg tgaaaataga taaaggttct gagataacga gaaacataaa gataatagaa
    16741 tggatgaaaa cagaaatatt aatgagtgta agtgatttgt ttaatttatt gtttaaaggg
    16801 gtaaaaccat tagatgaagc aattcaagat actttagcaa atataataat gataacatat
    16861 ctcttagcaa agagattagg aattagtttt agagatgtgg attataaagt caaggaaaaa
    16921 ataaaaattg gtatagatga ggaccatagc gttgaaagat ggtatggaga tttatctaat
    16981 ttaaaaaaac atatggataa tagggagtga tattaaattg aataataaaa ttagactatc
    17041 taaggcaatg tctataatag ttttagctat aataatcgct ttagtaatag cctatattcc
    17101 aatgctagga atatttagta tgttagcagc tgttccatat gtaattatag gtgctattac
    17161 tgatagaaag tattctctta tatctatttt ggttacattt tgtgtactga ttctatttgc
    17221 agacatttct tatgcattaa atatatgtat aatgtattct ttaccaggta tagtaatagg
    17281 aaagatgcta aagagaagct ttgaacaaga ggacagtaat aaattcgaac cgatatatgg
    17341 tggaaccata atatttgtac tttcaatgct tgtatatttt tttgtattaa agacattttt
    17401 aaatgtgaat ctattagatg aagtttctaa gatgatatca gaaattgtaa atattcaaaa
    17461 aaatagtttt tctgcaactg aacttaaagt gtttgataaa atgaagccag atgagattgt
    17521 aagttatttt acaaatatgc taccaatgat gttattttta caagggttgt tatcagcgtt
    17581 tgtcacatat tatttgagtg tattttttat aaaaaggatt acaaagatga atataagatt
    17641 tcctaaattt gctgattttt atttaccagg aaatgctata ctcacaactt tcttacttta
    17701 tttattagtc ttatttattc aaattattgg ttcaaaatta tatacagaat taataatgac
    17761 taatttacag ctagttttta atttaatgtt tgtaattcaa ggtatagcag tttgcattta
    17821 tttcttaaag aaatggataa gacagggacc aaataaaatt atgttttttt cagggataat
    17881 tttatgttta tttggattta tgggaatatc ttttgttggt atggtagata gtataattga
    17941 ctttagaaag gtaagaagct ataaatccac ttaggaggat agaaatgagt aacaaacaaa
    18001 cctttaaatt aaatatgcca gaaataaatt tatatataat tgttattggt atttctagta
    18061 taattttgct ttattacaac ttgtatgtag ggtgtttatt tttctgtatt tttgtatata
    18121 tggtttttca caattggaga actactaata ttagacgtca tgaatggaca gagtatattc
    18181 aaaatttatc tttagatata gatgaaacaa ctaaaaaagc cataataaat cttccaatac
    18241 cattatgtat tttagaattt gatggaaata tatcctggta taatggaaag ttttatgata
    18301 tgattggtca aaaagattta cttgataaaa atatagagga tatagttaaa aatcttaatt
    18361 taagaaaagt attaaatgaa aataaagaga tgtacacaga aataaactat aaagaaaaag
    18421 aatatacaat aatttataat gttataaaaa atgatcaaga aaaaaatcct aaatatttga
    18481 tgatattata ctggatagac aagactgaat atttaaaagt aaaacaaaat tatgatgatg
    18541 aaaaaaatgc aatgatgcta atacaagtag atggatatga tgaagtatta aaaagtgcag
    18601 cagaagataa gagagcttta attaatgttg aagtagagaa aatactttct gctttagaat
    18661 taaattcaaa tggtgcattg agaagaactt caaaagacaa attcttcttg gtaatgcata
    18721 aaaaagagct aaaaaaattg gaagctgaga agttttctat tttagataca ataagacata
    18781 ttgattatgg aaataatctt cctgttacta taagtatagg aattggtata gatggagata
    18841 cattaaatga gaacctaaaa cttgctacgg gtgctctcga tttagcactt ggtagaggtg
    18901 gtgatcaggc tgttgtaaaa acaaaagata aatttgtgtt ttatggagga aaatctaaag
    18961 ctgtagaaaa gaaaaccaaa gtaaaatcca gattaatagg tcatgcttta agagaggtta
    19021 tacaacaaag tgaccaagtt tatataatgg ggcataaata tcctgatatg gatgctatgg
    19081 gagcagcagt tggtgtttat gatatatgta aatcctgtaa caaaactgca aacatagttt
    19141 tacaatctgt aaatgaatct atagaaatat ttataaataa aataaatgaa aataattact
    19201 ataaaaagct gtttattgga aaagaggaag ctattgacaa ctgtactaaa aatacattag
    19261 ttgtagtagt tgatactcat agaccaaatt atactgaatg tgaagaactg ttaaagctat
    19321 cagagaaggt agtagttata gaccatcata gaagaggtgt ggagtttata aatgatgcag
    19381 tgcttttatt ccatgagata tatgtatcct caacgtgtga aatggttaca gagttagttc
    19441 agtatatgga tgaagatgta acaataaata aacttacagc tgaagggctt ttagcaggta
    19501 taagtctaga tactaaaaac tttgcattca aaacaggtgt taggactttt gaagctgctt
    19561 cttaccttag aaaagttgga gcagatacta tagaggtaaa gaaattcttc aattctgatg
    19621 taaaagattt cataataaaa gctgaaataa ttcaaagtac taaaataatt aataatagaa
    19681 tatgtttagc atattcaagt actgagatag atagtataaa tgttataata gctcaaactg
    19741 ctgatgagtt attaaatatc aaagaagtag aagcttcctt tgtattaggt gaaaaagatg
    19801 atactatatt tataagtgcg aggtctttag ggcaaataaa tgtacatgta cttatggaaa
    19861 aattaggagg cgggggacac atagatatag ctggagcaca gttaaaaaat gtttctctaa
    19921 aagaagctta taagatggta aataaaataa tagaggagta tttggaggag gaagaataga
    19981 tgaaagttat attattaaaa gacgtaaaag gaactggtaa aaagggagaa atgaaagaag
    20041 taagtgatgg atatgcaaga aatttcttat ttcctaaaaa gatggctgtg caagcagata
    20101 gtgtagcaat aaaagaatta aatgaaaaga ataaatctaa agaaataaaa gcacaaaaag
    20161 agtatgaaga agcagtttta ttaggtaagc aaatggaaga aattaatata gaaatatatt
    20221 caaaagcagg agaaggtgga agattatttg gttcaataac ttctaaagaa attgctgagc
    20281 aattaaagaa acaaaaagac atagacgtag ataaaagaaa aatattatta gatgaaccaa
    20341 taagatcttt aggttcaact ttcgtagaaa taaaaataca tcaaaaagta actactaaaa
    20401 taagagtgga tgtaaaagaa aaacagtaat aactagaggt gattaagatg gaagatatga
    20461 cgagaattcc tcctcatagt gtagaatcag aacaatcaat attgggttct atacttctag
    20521 ataaagatgc tataataacg gttacagaaa ctataaagcc tgatgatttc tataaagagg
    20581 ctcacaaaat aatatatgaa tgtatgataa ctttaagtaa taaaggtgag cctatagact
    20641 tgataacatt aacagaagaa ctaagaaaac aagggcatct taatgatatt ggaggaatca
    20701 gttatattac aagtctttct actatagtac cgacgacttc aaatgtaaaa tactatgctg
    20761 atatagtaaa agaaaagtct gtacttagaa agcttataaa agcttctaat gaaataataa
    20821 atctaggata tagtggagca actaagattg aagatgtttt agaacaagct gaaaagagta
    20881 tatttgatat atcacaggag aaaactagtg atgattttaa atctataaac ttagttttga
    20941 tggatgccta tgatatgata gaaaaattat atactaacaa gagtgatgtc actggtataa
    21001 ctactggttt taaagattta aacaaaaaaa taaatggtct tcaaaggact gatttaatat
    21061 taattgcagc tagaccagca atgggtaaaa ctgccttttc tttaaacttg gttcaaaatg
    21121 cagccttaaa aggtgatgcc tctgtagctg tatttagctt agagatgtct aaggaacaat
    21181 tagtacaacg tatgttatct tcacaatcta gtgtagaatt aaaaaaaata aagactggaa
    21241 cacttaatga taatgattgg cctagaatta tagatgctat ggcagtatta tcagatgcca
    21301 aaatacacat agatgatact cctggtataa aaatatcaga actaaggtca aagtgtagaa
    21361 agttaaaaat agaaaaagga ctagatttag tgcttattga ttatcttcaa cttatggaag
    21421 gtgaaggtaa taatgaaagc agacaacaag agatatctaa gatatcaagg tcactaaaaa
    21481 tattagcaaa agaattaaac tgtcctgtag ttgcactatc tcagttatca cgtgcaccag
    21541 aacaaagagc agaccataga cctatgttat ctgatttaag agagtctgga gcaatagaac
    21601 aggatgcaga tatagttatg ttcttataca gggacgaata ttatcatgct gattctgaga
    21661 gtaaaaatat aggagaagta ataatagcaa aaaatagaca cggtgaaact ggttctgtgg
    21721 aacttgtttg gcttggtgaa gttcaaagat ttggggataa gttgagagac ctataatcta
    21781 acgaatatcc acagaacgtc ctttctttct acaggaaaaa tgtggataca tgtcactaaa
    21841 tatgtccaaa cattgaaatt tagctaaaaa actatccaca ctatccacag atagcctgtt
    21901 gataagtgtg aataagtaga taaagcaagc ttttggcttg ctttatctgt gtataaatat
    21961 taattattag gaggaataat atggaaaaaa taagcttaaa atatatatat cccaatataa
    22021 ttaaagtact tgatgaaata aacttattta gagtaattga caataattta agagagtcta
    22081 ttgttgttta tgctaataat gtggataatc agtatcatat aaacatgact aatacaaact
    22141 ttggaaacat aattaacata tgtaaattag aaaaactttt agatgtggat aaatttatgg
    22201 aaaaagttat aaaatatgaa aaagaaataa tagagaaaga agagttttca aaaatagaag
    22261 aatatatgtt aaatattgga gaatactaaa aaagttgaaa aaaatacgaa tgtttgatag
    22321 aataagtaag taaagtgttc gagagtatat aaaatataga ggtgaattac atgaaaacag
    22381 ttgcaattgt tggttctcaa tggggagatg aaggtaaagg taaagttata gattatcttg
    22441 ctactcaagc agatgtagta gtaagagggc aaggtggaaa taatgcagga catacattag
    22501 ttgtagaagg taaaaaatac gcattacatc taattccatc tggagtttta aatccaaata
    22561 cagtgaatat aatcggaaat ggaatagtgt ttgaccctaa aggattttta gaagaattgg
    22621 aaatgtttaa aacggataat ataagtacag aaaatataaa aataagtgat agagcacatg
    22681 tcatattccc atatcataaa gaactagatg cattgtcaga agaagccaga ggcgatttaa
    22741 agataggtac aacaaaaaaa ggtattggac cttgttatat ggataaaaca gagagatctg
    22801 gtattagaat atgtgactta atggataaag ataaatttgc tataaaatta aaagctcaaa
    22861 tagatgctaa aaataaaata gttaaaaata tatatggaaa agaagaatta tttgattttg
    22921 aaactatata taacgaatat ctaggatatg cagaacaaat aagaaaatat gtggcagata
    22981 cgtctgttat tgtatatgat gcagtaagag caggtaaaaa agtattgttt gaaggagcac
    23041 aagggacttt attagattta gatttaggaa cttatccatt tgttacatct tctcatccaa
    23101 catctggtgg atttgcaatt ggtgcaggta taggaccaaa tatgataaaa gacgttgttg
    23161 gtatagtaaa agcatatact acaagagttg gagaaggacc gtttgttaca gagcaaataa
    23221 atgaaactgg agataagata agagaacaag gacatgaatt tggggtaact actggtagac
    23281 caagaagatg tgggtggttt gatgcagtta tagttaagta tgcagctaga gttaatggat
    23341 tgactagtat ttcatttatg ttacttgatg tattaactgg atttgataag ataaaagttt
    23401 gtacgtctta taaaatgggc gataagataa taacggattt cccagcttca ttagatgatt
    23461 tagcaaaatg tgaacctgta tatgaagaat tagatggatg gaatgaagat ataactcaaa
    23521 tagataactt tgatgacctt ccagaaaatg caaagaaata tgtagcaaaa atagaagaac
    23581 tagttggagt aagtgtagat atggtttctg ttggtccaaa tagagctcaa actataataa
    23641 gaagaaatat atttgcttaa tattttatca attaaataat ttaaaatagt tgaagaagag
    23701 tatctcaaga taatgtttat agttattaga gatgctcttt tatatttgac ttacattgat
    23761 tttttattaa gttaaagcta cttatacata ttctagctga aattaaagtt gaatttatgt
    23821 agatacatat aggtttttta aaaagaattt tgttgaaaat aaatggctaa agatgatttt
    23881 aattatattc taaaaaaagt aaaaaaaact attgactaaa aattgtatag acgctataat
    23941 ataacttgtg agtgaaagaa acgacgaaaa aagtcgaaaa aatgtggtct attagctcag
    24001 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
    24061 aaaatatggc tcgttggtca agaggttaag acaccgccct ttcacggcgg taacaggggt
    24121 tcaattcccc tacgagtcac cagtatggga ctatagctca gctgggagag cacctgcctt
    24181 acaagcaggg ggtcacaggt tcgagccctg ttagtcccac caaattttga attagagata
    24241 aatactagat tatatgctgg tgtggctcaa cggtagagca gctgacttgt aatcagcagg
    24301 ttgtaggttc gattcctatc accagctcca caaagtacat aaattcaaat taatataatt
    24361 ttctatgtta caggatactt ttaagtatct ttttttaatt tc
//
LOCUS       DAELSY010000051        24353 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.051, whole genome shotgun sequence.
ACCESSION   DAELSY010000051 DAELSY010000000
VERSION     DAELSY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24353
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.051"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(389..1219)
                     /locus_tag="KPG09_001791"
     CDS             complement(389..1219)
                     /locus_tag="KPG09_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0966404.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPITYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKI
                     "
     gene            1292..3019
                     /locus_tag="KPG09_001792"
     CDS             1292..3019
                     /locus_tag="KPG09_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0966405.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLIMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFVKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNREHFEKKRF
                     DRTAIDYSETAISINKVFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALI
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGRKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDVKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEIKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            3056..4918
                     /locus_tag="KPG09_001793"
     CDS             3056..4918
                     /locus_tag="KPG09_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0966406.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKVKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRRAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFSSEYDEEVV"
     gene            5251..5940
                     /locus_tag="KPG09_001794"
     CDS             5251..5940
                     /locus_tag="KPG09_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBG0966407.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNNCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSIDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            6045..6731
                     /locus_tag="KPG09_001795"
     CDS             6045..6731
                     /locus_tag="KPG09_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBG0966408.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKYGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            6748..8655
                     /locus_tag="KPG09_001796"
     CDS             6748..8655
                     /locus_tag="KPG09_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBG0966409.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLTSVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETHKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDKKEVNAELTSSVVKT
                     NTDNETENKTTNDSESKNNTDKDKSGNDNNQKLEGPRGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            9108..9797
                     /locus_tag="KPG09_001797"
     CDS             9108..9797
                     /locus_tag="KPG09_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966410.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            10233..10772
                     /locus_tag="KPG09_001798"
     CDS             10233..10772
                     /locus_tag="KPG09_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0966411.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            10791..11834
                     /locus_tag="KPG09_001799"
     CDS             10791..11834
                     /locus_tag="KPG09_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0966412.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            11836..12672
                     /locus_tag="KPG09_001800"
     CDS             11836..12672
                     /locus_tag="KPG09_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0966413.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            12777..13451
                     /locus_tag="KPG09_001801"
     CDS             12777..13451
                     /locus_tag="KPG09_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0966414.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            13451..14503
                     /locus_tag="KPG09_001802"
     CDS             13451..14503
                     /locus_tag="KPG09_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0966415.1"
                     /translation="MIKFKKTISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            14947..15795
                     /locus_tag="KPG09_001803"
     CDS             14947..15795
                     /locus_tag="KPG09_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0966416.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            15785..17449
                     /locus_tag="KPG09_001804"
     CDS             15785..17449
                     /locus_tag="KPG09_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0966417.1"
                     /translation="MMFKIFNKSLLKLVIVLSIVLTISNFSLFSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINENYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKVTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     ITKSDTRFKYISNISKILIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            17498..18754
                     /locus_tag="KPG09_001805"
     CDS             17498..18754
                     /locus_tag="KPG09_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0966418.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYKVSKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIEGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTTVIISDILCVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            18777..20858
                     /locus_tag="KPG09_001806"
     CDS             18777..20858
                     /locus_tag="KPG09_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBG0966419.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASSFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGTNINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKPKKEVKDSSWNMSINKTTKVFLMISFITIIVVMIL
                     SMLYIKKYKRR"
     gene            20895..21980
                     /locus_tag="KPG09_001807"
     CDS             20895..21980
                     /locus_tag="KPG09_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG0966420.1"
                     /translation="MRKYMNKFKLKRSILISLIILIFIFIVYFVSIELKDGSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWVENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDSSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            22034..23158
                     /gene="wecB"
                     /locus_tag="KPG09_001808"
     CDS             22034..23158
                     /gene="wecB"
                     /locus_tag="KPG09_001808"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBG0966421.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKALVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDSYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            23236..24210
                     /locus_tag="KPG09_001809"
     CDS             23236..24210
                     /locus_tag="KPG09_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG0966422.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSVTIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDIIRSYNLQLIDNKVETK"
BASE COUNT         9698 a         2448 c         4181 g         8026 t
ORIGIN      
        1 ataccacttg aattaagtct taaagcatta aagtttaaaa aagcattatt taaatcggta
       61 tccaaaagaa aaggatgctt tgatttgcta tatatggaaa aattaaaaat atattcataa
      121 tcaaatttat aaaattaata tctttccatc ctaacattac atttacttag attaattatt
      181 attataaaaa taaaaataca aataatagat tcatttacat tcttaaatga ttaaagctct
      241 ttacccttta aacatgcata acatatcgtt ataattccag taaataagac taaaatgccc
      301 attcctaaaa ttgcagtttc atcatctatt actccaaatg cttttaaagt aacttatttc
      361 gctttgtata ataataattt gtctcttttt atattttaac ttctgctgag atttgagtta
      421 gatattcgtt agaatctcgc acacttatat catatagaag atattcttca taaacaaaat
      481 ccccaatacg taaattattc tgattaaaaa aatttaataa cttttcataa gccttgtaaa
      541 tagtttcata actaccttta tgataagcag ttacataaag tccctttggt ttgattgata
      601 caggatattt cttgtactct ttattaactt ttgtaaataa atatgaatat gattcaaagt
      661 ctttattaat tatatcttca cctttaatga ttgagccaat cgtaccatag tcactcaatt
      721 catagttgtc cataaagtta atacattcat ataaaaaatt ttcttcctca tctcctaaaa
      781 actgttcttt atatgtaata ggtgttttga ccaagtactc ttcctcatgt tcttttaaag
      841 taatagtatt cgcatctata ttacaggcat ttttagtgaa tgaaatagta gattccatta
      901 gagcttgaat attatttagt ttttcaattt catttttaat atcaatggtt ttcttttcta
      961 aaagctcaat tagtaaatta ggtgttcttt tgtctatata agctttaatt tcttttaaag
     1021 gcatttttac ttccctaagt gaagaaatta taccaaaaat gctaagttga tggtatgagt
     1081 agtatctata tccattttct ttctttaatt ctggtgagaa aagacctata tcatcataat
     1141 ggaaaagtgt ttttttattg attccacaaa tctttgcaaa ttctccagtt gtaaaataat
     1201 tttttgaaac ttgactcata attataattc ctccttgact atcctgttac agtatagttt
     1261 atcatagtat tttgtaaagg agtgtgatta tatgaaaaaa ttaattcatt ttttaaaacc
     1321 atatagagtt ttaattgttg tagttttgat atttacgttt ttacaaacac ttggaacttt
     1381 gtatatacca acacttacag caaatatagt aaacaatggt gtagtaaagg gagatataga
     1441 ttatattgta aaaacaggtt taataatgat gatagtagct ggtataacag ctttatcagc
     1501 tgtgttagtt tgtaaagtat cagcaaatct ttcatctgga ttttgtagag atattagaga
     1561 agcagtattt gtaaaatcac aagatttatc aataaatgat tttaataata taggtacagc
     1621 atcaatgatt actaggagta ctagtgatat tactttaata ggtcagtctg tgtttatgtt
     1681 tattcaattg gtattaccag caccaattat aactgtttct ggtctattct tagcatattc
     1741 tatagataaa gcaatgacaa ttattattgt tgtagtaatg tttttattca tgctttcagc
     1801 atttttagtt ggtaaaaagc ttataaagtt gtttaaaatg atgcaaataa agatggacaa
     1861 tatgaatagg gtattacgtg aggtagtaac tggagtaaga gttataagag cttttaatag
     1921 ggagcatttt gaaaaaaaga gatttgatag aactgcaata gattattctg aaacagcaat
     1981 ttcaataaat aaagtatttg cagtacttat gcctattgta atgttaataa tgaatttagg
     2041 tatagtaagt attatttggt ttggtgggat gagagtcagt aatggaaata tggagatagg
     2101 tcatataatg gcacttattg aatattgtat cttaatttta ttctatttaa tcatgggagt
     2161 aatggtattt atgtacatac ctagagcagg tgcatgtgca gatagagtaa atcagatatt
     2221 agatatagaa cctgaaattg tagatggaaa tggtcgtaaa gatactgtct cagagagggg
     2281 acatttagta tttaaaaatg taacattttc atatgcacaa tcagaagaac ctgtgttaaa
     2341 taatataaca tttgaagcaa aatctggaga agtaactgca attataggaa gcactggctc
     2401 aggcaaatct actattgcaa atataatacc acgttttttt gaaattcaat ctggagaaat
     2461 atctataaat ggtcaggatg ttaagaagat tcctcaaaaa gaacttagag ataagattgg
     2521 atttgtgcca caaaaagctt ttttatttag tggaacaata gaagaaaata taaggtatgg
     2581 aaaagaagat gcaagtattg aagaaataaa gcatgctgca agtatagctc aagctgatga
     2641 atttatttct gatatggaag acaagtatga ctcatttgta gcacaaggtg gaaataattt
     2701 gtcaggtggt caaaaacaga gaatatccat tgcaagagcg ttagttagaa agcctgaagt
     2761 atatgtcttt gatgatagtt tttctgcatt agattttaaa acagataaaa gacttagaaa
     2821 agcgttgaaa aatgaaatca aagattcatc agctataatt atagcacaac gtataagtac
     2881 aatcatggat gctaatcaga ttatagtttt aaatgatgga aaaattgtag gtattggaaa
     2941 acataaagat ttacttgaaa attgtgaagt ttataagcaa attgcggatt cacaacttag
     3001 taaagaagaa cttgcataaa atgaagaatt aatctgatat ttggaggtgt atgatatgag
     3061 tgaaaaaaga atagatgaac aagatataga agtacttagt tttgaagatg gcatgtcagc
     3121 agaaaaagtt aaggatgcaa aaaaaactac taagagatta ttaaaatata tggcaaaaca
     3181 aaagtttaaa atattaatta tttttatttc agtattgata agttcagcgc ttacagtttt
     3241 agcacctatg gttatgggaa aagctattga ccaactattt aatggaataa aaactgcagt
     3301 ccaaacaggt acaaaattca gtgtgaattt tagtactatg ggggggatag tatcaatact
     3361 tctagggttg tacttaatta gtgcagtatt tatatatatt caacagtata ttatgtcagg
     3421 agttgcacaa aatctagttc ttagcatgag aaaagattta agtgataaac tcaataaact
     3481 tcctcttaag tactatgatt ctcataaaaa gggggaaaca cttagtatag taacaaatga
     3541 tttagaaaaa gtagcagata gtttacaaga aggattaatg caacttatta cagcagtagt
     3601 tacagtagtt ggttctattg taatgatgat aagtataagt gtaccactta caatagtatc
     3661 agctataaca ttacttgttt cattgggtat tactgttgta attgcaagaa aatcacaaga
     3721 aagattctct gaaaatcaaa aggcactagg agagttaaat agcaatatag aagagatatt
     3781 tactggtcaa atagtggtta aagcatttag taaagaaaag gatactataa gaaactttaa
     3841 aatattaaat caaaatcttt ataatgcaag tagaagagca cagttttcgt catatgcaat
     3901 atctccaatt attagattta taaacctaat tggctatgtt attatagcag ttgtaggtgg
     3961 gatatttgca tcaactggag caatgactct tggtagtata caagcgttta ttcaatatgt
     4021 aaatcaagca tcagagccta ctacagaaat atcttatatt gtaaatatgc ttcaagcagc
     4081 aatagcatct gcagagagag tttttacagt tatggatgag gtggaagaga taaaggatga
     4141 agaatcttca aaggttatta gcatgcctaa aggtaaagta caatttgagc atgttaaatt
     4201 tggatatagt gatgatttta ttcttatgaa agatattaat atcaatctaa atgcaggaga
     4261 caagattgca attgttggac ctacaggagc tggaaagaca actcttgtaa atttacttat
     4321 gagattttat gagatacaag gtggaagaat tactattgat ggtgttaata taaaagactt
     4381 aaagcgtgga gaacttagaa ctatgtttgg tatggttctt caagatacat ggctgtttaa
     4441 tggaagtata aaagaaaata ttgcatatag taagtcagat gccacaatgg atgaaattgt
     4501 aagagcagca aaatcagcaa gagtagacca ttttataagg actttaccac aagggtatga
     4561 cactatattg actgaggatg cttctaatct atctcaaggt caaaagcaac ttctaacaat
     4621 tgccagggca atactttcag acccatcagt attgattctt gatgaagcaa catctagtgt
     4681 agatactaga acagaggtag aaattcaaaa ggcaatgaat aacttgatga agggaagaac
     4741 tagttttgtt attgcacata gattatcaac tattcgagat gctgatttaa tattagtaat
     4801 gaaagaaggc actattattg aacaaggaag ccataaggaa cttattgaaa agaaaggatt
     4861 ctatgaagaa ctttataata gtcaattttc aagtgaatat gatgaagaag ttgtatgatt
     4921 atcaagtttt aggcaactaa atgattgata aatattaagt taaatatagt aacatgtaag
     4981 gttatatgga ataagagtat ttctctattt catataacct tttttattta tcaaaataag
     5041 ataaaatttt ctttctaaat tagattgaag aaagtattta tcaatataaa agtaaaataa
     5101 tcattattat tagtgaagta aatattgttt gccacatata tgccataaag atgcacttat
     5161 tttacggaga tttaacaaag tagtgtaaag tatactctaa atataaaata aatcaaatag
     5221 agttaaataa attgagaggt gatataaata gtgaataaaa aattgaacta tagatttgaa
     5281 atgaagcaca aaataactga agcagatgtg ttagctttaa aatcaagact ctatccaata
     5341 atgaagaaag atgaaaatgc atctaaagat ggaaaatatc ttattaggag tttatatttt
     5401 gatactcctg aagataaagc acttttagat aaattaaatg gcgttgcaat aagagaaaag
     5461 tttaggataa gattttataa taataactgc tcttatataa ggttagagaa aaaaatcaaa
     5521 cattacaaca tgacatcaaa gctatcaact aatttaacca aaaaagaagt aataaatata
     5581 ctaaacaatg atattgaatt tctaaaagag tcgacaaatt gtttattaag agaattttat
     5641 ttgaaattaa aatgtgaaag attagaggca aaaagcatag ttgattacaa tagagaagct
     5701 tttatttatc cagttggaaa tgtaagagtt acaatcgata gtgatattaa aacttctgta
     5761 aattcgatag atttatttaa taaagatttg ccgactgttt cggtaattga tgagaatatg
     5821 actgtattgg aggttaaata tgatgaattt ataccagatt ttataaagga tttaattcaa
     5881 attaataaga ctacttctac agcagtttca aagtatgctt caagtagatt atatatttaa
     5941 gaataaaggc tatgatttat ataaagatat acataaataa ggttaaaaaa caaataggtt
     6001 aaaaataaat aaaataaaaa ataaataagt taaaaatagg aaggatgatt ttaattatga
     6061 catttaatga tatttttaaa tcaagtttta tagaaaacgt atcaggtttt tcatttgtgg
     6121 acagtgcttt agcactaggt tcagcttttt tagtaggttt gtttatatat atggtctata
     6181 aaaagactta tatgggaatt atgtattctc gaccattcaa tgtgtcgctt gtagcactta
     6241 caatgcttac aacttttgta atattggcgg ttacttcaaa tgtagtttta tccttaggta
     6301 tggttggtgc tttatctata gtaagattta gaacagcaat taaagaccca atggatttag
     6361 tatttctatt ttggtcattg ggttcaggta tagttttagg agcaggtttg attcccttag
     6421 ctgtcatggg ttcaataatt atgggattga ttctaatatt cttttcaaat aaaacaatat
     6481 ctgaaactcc atatatattg atggtaaatt gtaataatga agattcagaa gatatagcaa
     6541 ctgataaaat taaaaaagta tttactaaat atcaaataaa atcaaaaagt gttacaccag
     6601 aaaaaggaat agaattagtg tttgaagtaa gaatgaaata tggagagaca agtcttataa
     6661 atgacttgag tcaagtagat ggagttacta atgcagttct tgtaagctat aatggtgact
     6721 atgtagcata gagaggagta atcttttatg aaagataaaa aatttaccct tcttatctct
     6781 attatgatta tatttttatg tgctgtagtt ggagtttata gtacatctag caacaaaagt
     6841 gttgatttat atagtgatgt atatattgaa aaatatttta acagagacaa ggttatggaa
     6901 gttaatatag agatagatga aagtgacttg aaggatatga atgaaaatgc tataaaagaa
     6961 gaatttaagg ttgcaaaagt aactgtagat ggagatacat atggaaacgt aggtataaga
     7021 actaaaggaa attcaagtct tacatctgta gcaaatagtg atagtgatag atacagctat
     7081 aagattaatt ttgataagta taatactagt caaagtatgg aagggcttac tcaattaaat
     7141 cttaataact gttactctga cccatcttat atgagagagt ttttaacata tagtatttgc
     7201 gaggaaatgg gattagcgac tccagaattt gcatatgcta aagtctctat aaatggcgaa
     7261 tatcatggtt tgtatttggc agtagaagga ttaaaagagt cttatcttga aaataatttt
     7321 ggtaatgtaa ctggagactt atataagtca gatgaaggaa gctcgttgca atataaagga
     7381 gatgacccag aaagttactc aaacttaatc gttgaaagtg ataaaaagac agctgattgg
     7441 tctaaaatta caaaactatt aaaatctttg gatacaggtg aagatattga aaaatatctt
     7501 gatgtagatt ctgtccttaa aaatatagca ataaatacag ctttattaaa ccttgatagc
     7561 tatcaaggca gttttgccca taactattat ttatatgagc aagatggagt attttctatg
     7621 ttaccatggg attttaatat gtcatttggt ggatttagtg gttttggtgg aggtagtcaa
     7681 tctatagcaa ttgatgaacc tacgacaggt aatttagaag acagacctct catatcctcg
     7741 ttattaaaaa atgagacaca caaaacaaaa taccataaat atctggaaga gatagtaaca
     7801 aaatacctag attcagacta tttagagaat atgacaacaa aattgcatga catgatagca
     7861 tcatatgtaa aagaagaccc aacagcattt tatacttatg aagaatttga aaaaaatata
     7921 acatcttcaa ttgaagattc tagtgataat aagggatttg gtaataaagg gtttgacaac
     7981 aataactcta ataacagtga ttctaataat aattctaata gtgaaaataa gcgctctgga
     8041 aatcaaagtg ataaaaaaga agttaatgct gaattaacat caagcgtagt caaaactaat
     8101 acagataatg aaactgaaaa taaaactaca aatgatagcg aaagtaagaa taatacagat
     8161 aaagataaaa gtggaaatga taataatcaa aagctagaag gtcctagggg taaaggaggt
     8221 aagtcaatac caggggtttt ggaagttgca gaagatatga gtaaaactat aaaatctcaa
     8281 ttaagtggag aaacttcttc gacaaagcaa aactctggtg atgaaagttc aagtggaatt
     8341 aaaggtagtg aaaagtttga tgaggatatg agtggtatgc cagaaccacc tgagggaatg
     8401 gatggtaaaa tgccaccagg aatgggtaat atggataagg gagatatgaa tggtaaaaat
     8461 ggcaatatga atatggatag aaatcaagat aatccaagag aagctggagg ttttggcaat
     8521 agaggaggag gctctgtgag taaaacaaca acatacttca aattaatttt aggtggagct
     8581 tcaatgataa taatgtcgat tatgttagta ggtgtatcaa gggtaaagag aagaagattt
     8641 ataaagtcaa aataagatta aaattgaata taaataattt taatactgtt aagtctaaat
     8701 atttacttag cagtattttt ttagaaaata atgtgtttga ttaataatat aaggtaataa
     8761 attgataaaa attaaataca gcatattaat aatacataat aaagtaataa aaaaactgtt
     8821 taagtataat aacttatatt tagacagttt ttttcattta taaataatat aaatgaaatt
     8881 gaaaacattt gaaggataga aattggttgg ataaatgtat aaattacttc accaaaaaat
     8941 aatgacaaga gttgtaacta agttgtaata aaatggtagt tatttagcat aaaataatgt
     9001 atcatagaag tatattataa aattaataaa ataagatttt tgtaagaaaa ctgctggctt
     9061 ttgatattta gagtgagtag ttaaattatg tttaggaggg gtagaatttg ttgcaaaaag
     9121 tattatggaa atttttaaag ctagtagtcg gtttatttat atgttcagtt ggtatagtat
     9181 tgacgataaa ttgtaacctt ggattatctc cctgggatgt atttcatcaa ggactttcaa
     9241 atcatatagg aataactata ggaactgctt ctataattgt aggctctata gtagttattg
     9301 ctgatgtcgt tcttggagaa aatgtaggat ggggaactgt ttttaacatg ctactaattg
     9361 gattctttat ggatttactt ttatatagta atttaattcc agaggctgat agtttatttg
     9421 taggaataat tatgttgata ttaggattgg ttcttttgtc tgttggaatg gtattttata
     9481 tgggaagtgg tttaggaagt ggacctagag atggtttgat ggttgcaatt caaaagaaaa
     9541 caggaaaatc acttaaatta ataagaggta ctatagaagt tggagcatta atagtaggat
     9601 ttttattagg tggaaaagtt ggtataggaa ctataataag tgcatttgga ttggggtatt
     9661 tcacacaaat ggtatttggc ttatttaaat tagattgtag taagattaaa catagattta
     9721 tagtagatga tataaaattt atcaaagtgt atatgagtgg tgagaaaaaa gctgctgttc
     9781 aaaatatagt taagtaattt aagaataaat tcaacgaaat gttaagtatt ttaaattaac
     9841 attttattta gatggataaa ttgaatatta aaagaatatt aaaatatgag gattgctaga
     9901 acactataaa atatagattt ctagcaattt tttatttgtt caatgttttt ttagtactag
     9961 aagtcttatt ctctatttaa tgaatgaatt aatttataat tagagatact aaactttata
    10021 gatattaata taaacacctt taaactgtgt attttccaaa gcctttaaaa tgcttgtatt
    10081 tattctaaat aaaaaaataa aaaaatgtca aaaaaactct tgtattatat ccaaatttta
    10141 tatataatta cctatgttgt ttagaaattt taaactcaat gtttagtaaa caaaatgttt
    10201 agtaaaataa aacagaaact aggaggtgca acatggatat aggtgaaaag ataaaaagat
    10261 taagaacaga aaaacaactg acccaggaag agctagctaa tagatgtgaa ttgtcaaagg
    10321 gatttatttc tcaattagaa aataacttaa catcaccatc catagctacc cttatagata
    10381 tacttgaaat actaggtaca aatttaaggg aattttttaa tgaaatagat gatgaaagaa
    10441 tcagttttac aaaagaagat atgtttgaaa ctgaagatga agatttaaaa tataaattaa
    10501 agtggttaat tccaaattca caaaaaaatg aaatggagcc aataataata acattgtatc
    10561 caggaggaca gtataaagaa gaaaagccac atgaaggtga agaatttgga tatgtattag
    10621 caggttcaat atatgtccat ataggcgaaa agaaaaataa agtaaaaaaa ggagagagtt
    10681 tttactttag accaaaagca aatcactata tatcaaacga aggcaaaact acagcaaaag
    10741 tgatttgggt aagtacgcca ccatcatttt agaatagagg tgtttaataa ttggtagaaa
    10801 atataataga attaagggat atatctaaac attatgagga acttactgtt ttagataatt
    10861 tcaatttaga tataaagaaa aatgagtttt taacattact tggaccaagt ggatgtggaa
    10921 aaactacaac attaaaaata atagcaggat ttgagtatgc tgatgatggt aaagttttat
    10981 ttgaaggtaa ggagataaat aacttgccac catatgaaag acaagtaaac acagtatttc
    11041 aaaaatatgc tttatttcca cacatggatg tttatgaaaa tgtagctttt ggattaaaaa
    11101 ttaaaaagac tccaaaagat gtaatagatg ctaaagtaaa agaggttttg aaacttgttg
    11161 cactagaagg ttttgaaaga cgacaagttg aatcattgag tggaggacaa caacagagaa
    11221 ttgcaatagc aagagctcta gtcaatgaac caaaggtatt attactagat gaaccacttg
    11281 gagcacttga tttaaagtta agacaagaga tgcaaataga attaaagaga atccaaaaaa
    11341 aattaggaat aacatttgtt tttgtaactc atgaccaaga agaggctctt actatgtcag
    11401 atactattgt tgtaatgtat aagggcaaaa ttcaacaaat gggaacacca caagatatat
    11461 acaatgaacc taaaaattca tttgtagcta aatttatagg tgaaagtaac atatttgatg
    11521 gaattatgat agaagattat aaagttaatt tctgtaatag agattttgag tgtgttgata
    11581 aaggattcga aaaaaatgaa aatatagaag tagtaataag accagaagat ataaaaatgg
    11641 taaagccaga agaaggaatg ttaaagggga aagttacatc tacagtgttt aaaggagtgc
    11701 attacgagat agaattgaat gaaaatggaa gattgtggat acttcataat accaaaaatg
    11761 ctgaggttgg gactgaacta ggcatggata tatatccaga agatatccat ataatgagaa
    11821 aagagaataa ctaagatgaa aagaaaatct tttttagcat atccatatgt agtttggagt
    11881 gcgatatttg taataattcc tttaatatta gtggtatttt ttagttttac aaaagaatca
    11941 ggtggaggtt atgcctttac acttgagaat tataaagaag taattgaccc tatatatatg
    12001 aaagtttttg ggcgttctat tcttttagca ggaggagcta cattaatatg ccttatagtt
    12061 ggttatccag tggcatatat aatttcaaag gctcgtgtaa gtagaagagg ttcattaata
    12121 ttattgttta ttcttcctat gtggatgaac tttcttttaa gaacatatgc atgggttgct
    12181 atattaggaa aaaatggact tttaaataca tttttaggtt ggtttggaat acaaccatta
    12241 gcaattttgt atactaattt tgctatactt ttaggtatgg tgtataactt tttaccattt
    12301 atggttcttc caatatatac agcactatca aagatggata atgatttgat aaacgctgct
    12361 catgacttgg gtgcaaataa tatgacagtt tttagaaaaa ttatatttcc tttgagtttg
    12421 ccaggtgtaa tgtctggaat aactatggta tttatgccag cagtcactac ttttgcaata
    12481 tcaagactgt taggtggagg taaaataatg ctggttggag atttgattga acaacagttt
    12541 acagttgttg gagattggaa ctttggttca gctatatcga tatttatgat gatagtaata
    12601 ttaatatcta tgtctataat gtcaaaattt ggagatgaat ctgacaaaga aggcggtggg
    12661 ttactatttt aaaattaaag aaaataactt ctaatatata tttagcatta gtattcttat
    12721 ttttgtatgc acccatattt gctttggtac tattttcttt caatgattca aaatcaatgg
    12781 caagatggaa tggatttaca tggaagtggt atggtcaatt acttcaaaat gagagtataa
    12841 tgagtgcttt atattatact attgtaatag ctatattagc gtctgtaata tctacaatag
    12901 taggaactat aagtgctata ggaatacata agatgagagg taagagtaag aaattaatac
    12961 taaatgtaaa ctatcttcct attttaaata cagagatagt tacagcagta gctcttatga
    13021 gcctgtttgt atttgtaaaa atggaatttg gatttacaac aatgctattg gcacatataa
    13081 tgttttgttt gccatacgtt atactttcag ttttgccaaa aatgaagcaa ttacctgata
    13141 atatagaaga tgcagctatg gacttgggag caacacctat atacgctctt agaaaagtaa
    13201 ttttaccaca aataaaacca ggtatagttt ctggattttt aattgcattt actatgtcaa
    13261 tagatgactt tataataagt ttctttaatg ctggaaatgg tgtaagcaat ctttctatag
    13321 aaatctatgg aatggctaga agaggtataa aacctgaaat aaatgcatta tcaacaataa
    13381 tgtttgcagt ggttctaggt ttacttttac tagctaataa aaaagaatct atagtaaggg
    13441 ggattaaata atgattaagt ttaaaaaaac aatatccctt atagctgtag gtatgatgac
    13501 agttacttta tttacaggat gtaataaagc aactgattct acaaaagttt taaatgtata
    13561 taatgttgga gattatatag atgagtctct tatagataag tttgaaaaag aaacaggaat
    13621 agatgttcaa tattcaactt atgatactaa tgaaatgatg tatcaaaaag taaagagtgg
    13681 tagtacacac tatgatttag tatttccttc agactatatg gtagagaaaa tgaaaaatga
    13741 aggtcttttg gaaaaattag attttaagaa tatacctaat atgaaatatt tagataagag
    13801 ttttttaaat cctgtatacg acaaaactaa tgaatatagt gttgcatata tgtggggaac
    13861 tcttggaata ttgtacaata agaaagaagt taaagaccct atggatagct ggaatatact
    13921 ttggaatcca aagtataagg gaaatataat gatgtttgac tctgttagag atactatagg
    13981 gataactctt aaaaaattag gatatagcat gaatagtgta aatccaaaag aaataaatga
    14041 agctaaaaat cttttaatga aacaaaagga tttagtattg gcttatgtaa atgatgaagg
    14101 taaggataga ttgcttggtg gagaagtggc aatgggtatg ctgtattctg gagatgcagt
    14161 tacgctaatg gagcaaaatc ctgaccttga ttatgctata cctaaagagg gaactaataa
    14221 atgggtagat gctatgtgtg tacctacaac agcacaaaat aagaaagaag ctgagctgtt
    14281 tataaacttt ttattagacc cagagaatgc aaaagtaaat gctgagtaca ttgggtattc
    14341 aacacctaat acaggagcac taaaattatt agacccagaa ataactcaga atcctgttgc
    14401 atatccatct aaagaagtac ttgataaatg tgaaactttt gaagatattg gtgaaaatat
    14461 aaaattatat gacaaggctt ggatagagtt aaaatcaaaa tagggtcttg ttggtttaag
    14521 taaatttact taataaatac ttaataaaac tgttcttttt tagaaaattt attttctaat
    14581 tattgaacag ttttattttt tgtttaggtc atgtttttat tttgagttta tttttatgag
    14641 aatttaaagt acagtaagtt tcattgatta ctatttttat aataataact ataaagaggt
    14701 tgtctcgaaa ttgagataag cctctttttc attgttgaaa tgatgaaaac atgtttgatt
    14761 tcataaaaaa agagtgattc atgagctaaa gaatttgctt tgagacagta ttttttatta
    14821 agttatattc atatgcaatt taaatagttt attaataaat gaaaacttat ttgtataatg
    14881 attttaataa taattagaaa aaatgggcaa cttaatagaa taattggagg tgtaaattta
    14941 taaaaaatga ataggataaa taaaaaaata gatttattaa tattggcttt gctcgttttt
    15001 atatttttaa ttgttggaat agtaatacta tcgattagga cagaaaataa attgaatgtg
    15061 ttcataatgt tagcaataat tttttttata ataatgttaa cttatttatc aaattcagta
    15121 gtggggttga taacaagttc aataattata tttatgtata catcctatat tttatataac
    15181 aatattactc acaatatgga tgttgaattt atatcatata tgtggataat agcaacacca
    15241 gtatcttcaa ttataatggg taatctgaac aaaagcataa atgaattgca aaatacaaat
    15301 aaaaaactat ctgaacagta taaggaatta gtgactattg atagtgaaac tggacttaga
    15361 aatttaaaga ttttttataa tgatgtaaat atggagataa gtaaatctat tagacataat
    15421 actgattttt ctcttatgat agtaaaatta ccatactacg gaaacctaca gactatattt
    15481 ggtgaaaaca aaactaataa aatagtaaaa tatattggtt caaatattat agaatgtaca
    15541 agaaatgaag atataattta tagtttacaa aaagatatga tagggatact aatgccaaat
    15601 acatctttgg aaggttcgaa agttgttaaa gacagaataa agaaaaggat aaaagaatta
    15661 aatttagatt taaacaatag gggaaaatat gtaaatatag atgtcaaaat tgcattttta
    15721 caatataaga gtagttttgg agatagtata aattttaaga atatagttga agaggaactt
    15781 caatatgatg tttaaaatat ttaataaaag tttattaaaa ttagtaattg tattatcaat
    15841 agtactgaca atatcaaatt ttagcttatt ttcatatgca tcagaaatta cttccagcac
    15901 agataaggta ttaatcatat atgattctaa gaaagaaaca gcttataata gagatatttt
    15961 aaatattatg agaactctat tgggaagatt tagttcagat atagagctat taaaattgag
    16021 taactatgat ggtgagataa atgaaaatta ttacagtcat atatttattt taggaatcaa
    16081 tgaaaactca tataataatg ataaaaacac taaaaacttg attagttcct taaattcata
    16141 taaagtaact atatgttggt taggatatgg tatagaaaat cttttggaac ataaaaaata
    16201 caatttagac tatgtaggaa aaacaaacaa tatagttagt gtaaattata gaggtaaatc
    16261 atataacttg gatgaacatt atgtttttaa cattgtagaa tcaaaagata caagtaataa
    16321 agtaatagga agtattaatg ataccttaaa taagtatcca tatataataa atgataaaaa
    16381 tcttttttat gtatcaaaat tagatttaga tggagtttta ttttatatat tttgcgattc
    16441 acttaatgat atttttaata ttaagacgtt tgataaagga cgtatatttg taagaataga
    16501 agatgtacat gcttttagag aaccaaaaaa tcttgtagag atagcggatt atttaagtag
    16561 taagaatata ccatttacga ttgcattaat accagcatat gtaaatccta aaaatcataa
    16621 agtaataact ttatcagaaa gccctgaaat tgtcaaagca ataaaatata tgcaagataa
    16681 aggtggaact gtgatattgc atggatatac tcatcaatac aagaaagaag aagtttctgg
    16741 agaagggtat gaattttggg atggtaaaaa agatgaaccc ttaaaagaaa atatgaagat
    16801 atttgtcaaa gatagagttt tgaatggtct tagagtatgt atagagaatg gaatttatcc
    16861 tcttgcattt gaagcgccac attatgcaat ggaatcagag ggatataaag aattaaaaaa
    16921 atatttttct acttatatgg ggcaacatca aaataatgat aaaaaattta gcacaaatac
    16981 atatccttat attattaggg atacagagga gtttaatata tttatacccg aaaatttagg
    17041 atatatagac ccagaagaca agtttacttt tcaaaatata aaagaaaatt tagataaatt
    17101 atctattgta agaggtttta gtggagggtt cttctttcat tcatatttaa acatagagta
    17161 tctaaaaaac acaatagaat atttagaaaa acagaatata gaatttatga atttaagaga
    17221 ttttaataat tgggtaaaag ttgatgaaat acaaataaga aataatggag atgaaattat
    17281 agtcaactat gataaggact tagatgaaat aactaaaagt gatactagat ttaaatacat
    17341 atccaatatt agtaaaattt taatattcat agtatctata agtgttttaa tatttgtgat
    17401 tatatttata tattttaaga gaatagataa aaagaaattt ttaaaataaa tgattttata
    17461 ttagaatcaa tattttaaaa tattttagga gaaatttatg agagatttaa atgtatcaga
    17521 gtatttgttc gtatttagtc tgtttagtat atggtcttta ttgcttataa atattatttt
    17581 agctatgggt ggatatatat tttattttaa aaactttgat aaagaaataa aagaaattga
    17641 tgaatatcct atgatatcaa tacttgttcc tgctcataat gaagctaaag tgataggtag
    17701 aactgtggag tctttgttat tactcaatta tcctaagagt aagatggaac ttattgttat
    17761 aaatgacaat tcttctgata atagtaaaga aattttagaa aatataaagg ataggtacaa
    17821 caattataat ttcactataa taaatacaga tagtttaaca ggtggaaaag gaaagtctaa
    17881 tgcattaaat attggatata aggtttcaaa aggagatttt atagctgttt atgatgcaga
    17941 taacacacca gataaaaatg cacttagata tctagtacaa actatagtaa tgaatgatga
    18001 attaggtgct gttattggga aatttaggac tcgaaataaa aataaaaacc tattaaccaa
    18061 atttataaac atagaaacat taagctttca atggatgtca caagctggta gatggcaact
    18121 ttttaatcta tgtacaatac caggaactaa ctttatattg aggagaagta tcatagaaga
    18181 aattgaagga tgggatagta aagcaattgc agaagataca gaaataagtt ttaggatata
    18241 taaattggga tacaagataa agcttgtacc acaatctata acatgggaac aagagccaga
    18301 aactgttaag gtgtggataa agcaacgtac tagatgggca aaagggaata tatatgtatt
    18361 aatgaaatat ataaaaaata tatttaaaca gggaagaaat aagattgtgt ttgatatagc
    18421 ttactttttt tctgtatatt ttctattttt aacaactgta attatatccg atattttatg
    18481 tgttttaagt atatctaaac tagtagaaat ttctattcca attaactttt ttctaatatg
    18541 gatactatct tatcttttat ttattataga agtgagtata agccttacta tagaaaaagg
    18601 tgaagcaaca attgaaaata tattcatagt tgcaataatg tattttacat actctcaact
    18661 atggctgttt gtagctataa aagggatgat agagtatcta aaagatataa tttttaaaag
    18721 agaggttaaa tggtataaaa cagaaagatt ttgattcatg tacaggagga attaacatga
    18781 aaaaatttat tatatcaatt atatctttag tattgttttt ttcaaatata tcacttatat
    18841 acaaagtaaa tgctgatgag actaaagtta aaaattataa gtttgaaaga gatataacta
    18901 ttgatggtgt tataggaagt aatagtactt tttttgaagt aaataaaaat tgggatattg
    18961 aagaagtttt attgcatctt aattttagta agagccaaat attaaatgga gatgtttcaa
    19021 gtttaactgt attgataaat aatgtaccaa taaaatctat taaattaaat gctaagacaa
    19081 attataaaaa tacattagaa gtattagttc caaaagatta tataattcaa ggatataatg
    19141 aaataaaaat aaaaacatac aaaacaatat ctgataaaat ttgccaagat gattccaata
    19201 ctgggaactg gatggttatt cacaaagaat catatatatc aattagatat aagcagaaaa
    19261 aggtggaaaa ctcaataaat gaatatccgt atccatatgc agaaattgaa aataatcata
    19321 aattagatac aactatagta gtaccagata atatgactag aggagagact acagcagtgt
    19381 ttaatttggc atcatcattt ggaaaaataa caaaaaatga tgatttaaag ttagatgtta
    19441 aattatattc agaaatgaaa aattggtcag atgataatat tatatatatt ggtaaacctg
    19501 aaaatacagc agaagaaatt ttggatattt taagtataaa ggaacaaacc ctattatctt
    19561 caaactgtat aatcaagcaa gtagattcac catacaataa gaataagaag atgatggtgg
    19621 taattggtag taatgaagat gatttaataa aggcatcaaa tttattaata gaaaatagac
    19681 tttctaacca agttttatca tcatcagtgc ttgtaaacaa agagactaat ataaaaatta
    19741 atagggaaca aaaattaaat ttaggtcatt taacattaaa agacttagga tattctgact
    19801 ttttgttgga aggagcattt aatcaacaag ctttgtttga tgtcaaaata ccaacgggta
    19861 aggtattaga tgatggttca aagattatct taaatttaag atattctgac aatttagatt
    19921 ttgaaaaatc tttagttaca gtgtctataa atgatgttat tgtgggaagt aaaaaattgg
    19981 ataggtcaca ttctaataat gataagttag aattaaaaat acctaaagat atagataata
    20041 aaaactatta tcaagttaaa ttgacattta atttgagtat aaaaaattcg aattgtgtta
    20101 ctagagagag taataatcct tgggcatatg tatcaaataa ttcttatttg gcgttatcta
    20161 cgaaagaaaa tgaaacctta tcttttgaaa attatccgta tccatttgtt agagatgatg
    20221 agtttaatga tttaactgtg ataatgccag attattcagg gtcacaagct atgacctgga
    20281 tgtttagatt aggtgttact ttaggtacga atataaattc acataatgga aatataaatg
    20341 taattagagg aaaagaattt agtgacaagt ataaggatac taatattgta gtttttggag
    20401 tgccacataa taattctgtt ataaagatgt taaataataa tttaaatata aagttcgata
    20461 aaaattactc caattttata tcaaatgata agataagctt tattgatgat tatggaaaaa
    20521 atatttctac cattcaatta ataaaatcac catataataa tcaaaaaaat atcatggtaa
    20581 ttagttcaat gaatgaaaaa aacttatatt taggaatgga ttatttgttg aataaaagta
    20641 aagttaatga tttaaaagga gatacattga taattgatga atacggagag gtagaagatt
    20701 tagcttataa tttaaaacct aagaaagaag taaaagattc aagctggaat atgagtataa
    20761 ataagacaac taaagtattt ttaatgattt cttttattac tataattgta gtcatgatat
    20821 tatcaatgct atatataaaa aaatataaaa gaagataata tgataaattt attattttaa
    20881 aagaggtata taatttgagg aaatatatga ataaatttaa attaaaaaga agcattttaa
    20941 tctcattaat aatcttaatt tttatattta tagtgtactt tgtaagtatt gagttaaaag
    21001 atgggagtct agtaaataat caatataaag gtaagattaa atcggccaat ttatctgtag
    21061 attatgaaat aaatcaagtt atgaaggata tagataaatt gggattgaat acagtaaatg
    21121 tacctataat tataaatgta gagtccatta attctgatat tatgtcaata gataataata
    21181 gtaaagaaaa ggcaattaag ttaataaaaa aattaaataa aaaaggtatt tcaacaatat
    21241 tagaggcata tccatgggtt gaaaatggaa aattgtatga gactgactgg aaccctataa
    21301 ataaaaaaag atttttttat acttggcaaa atgccatatt aaatgaactt ataattgatg
    21361 tagcaaatcc actggatgta aatgttttaa atattggttc taattttgta catttggaag
    21421 aatatcaaca aaattggggt gaaataatag attttgtaca atccaaattt gatggtcttg
    21481 taacgtacag aactaattgg tggtatacaa aaaaagatga ttcaagtagt aagttgtttt
    21541 atgaacaaaa attgaacaat aatttttttg ataagttaga ttttatatct atagcagcat
    21601 attttgagtt aagtaataaa cctgttaata cagtggatga attaatttct gccttacact
    21661 catctactgt aaacaataga ggacaaaata taaaacaaga aatatataac ctttataaag
    21721 ctcatagaaa acctatattt tttggagaat taggtttttc aaatagagaa agtgcaagtt
    21781 ctcagccgtg gaatcatact ccttcaaaag ctgtaaatgg cgaagaacaa gcaaggtgtt
    21841 ttgaggcata taagaaagtt tttgaaaatg aagactggat aaatggtttt tctgtgtttt
    21901 gtgtaggtaa aatagatgac gaaaaaaatt tttacccatc aaaagagagt ataaaagtta
    21961 ttaagtcttg gtatgaataa ttttaaaagt gtagaattta tatagattaa atagatgatt
    22021 aggagagata taaatgaatg acatcaaggt tatgactgta tttggaacta gacctgaagc
    22081 tatcaaagta gcacctttaa taaaggaact tgaaaaaaga gagaatataa aaagtattgt
    22141 ttgcgttaca gctcaacata gagaaatgct tgaccaagtt atagaaacct tcaatataaa
    22201 tgtagactat gatttggata taatggaaaa aggacaaagc ctgaatgata taacatgtaa
    22261 gattttaaat aaacttccct tgattttaaa caaggaaaat ccaaatataa tattggtaca
    22321 tggagataca accacaactc tagctacaag tttgactgca ttttataata aagctcttgt
    22381 tggtcatata gaagctgggc ttagaaccta tgataaatat tctccttttc cagaggagtt
    22441 aaatagacaa cttacaggta ttattgctga tatgcacttt gctcctacta atttggctag
    22501 aaaaaacttg atttctgaag gaaaacctaa taataatata tttgtaactg gtaatactgc
    22561 tattgatgct ttaaagatga caataaaaga aaactataat catcctataa ttgatgagat
    22621 tggtaacgat aggatgattt tgttaacctc tcacagaaga gaaaatttgg gtaaacctat
    22681 gaaaaatata ttcagagcta taaagcgaat agtagatgat tttgaagatg tacagatagt
    22741 atatcccata catttaaatc ctaaaatacg aactatagct gatgagatat ttggaaaatt
    22801 tcctgaaaaa attcatatta tagagccttt agatgtagct gattttcata actttttaaa
    22861 taaatcttat atgataatga ctgatagtgg aggaatacag gaagaagctc catcattagg
    22921 taagccagtc cttgtgttgc gtgataaaac agaaagaaca gaaggaattg aagcaaagac
    22981 acttaaatta gtaggtacta atgaagatag aatatataat tcagtgagcg atttattaat
    23041 aaataaagat agctatgttc agatgagtaa agcatctaat ccatatggag atggaaatgc
    23101 aagcaaatat atagtggata taataattaa aaaatttaat tgcaaatatt taaattagat
    23161 aaggtctaaa acattaacta attgatataa gatttatgat aatattaagg aatacaattt
    23221 ttaaggggga gtaatatggc tagaaaatta ataaaaaatt taggtaaaag taaaagtgta
    23281 aagagagtga aacttttatt taagaagatt tttattactg tattcatagt agcaagtata
    23341 gttgctattt ttaatataac gaaatatttt gaggaattat acaaagtaag ggatttaaaa
    23401 agtactaaaa ttgaatacta tatggatgtg gctgatgaag caggagatgg aaaggtccaa
    23461 ttaagttgga aggccttact tgctatagac atggtaatac atgatgaaga tttaagtaat
    23521 ataaaaaaga aagacacatt ggatataggg gaaaagttta tagtagaaga taaaaatgat
    23581 aaaggcgaaa aggtgtataa ggtaaaaaag tttaataagg tattaagcga attgaaattt
    23641 gactcttctc aaaaaagtag agcaagaaaa tacatgaaag atttggaata cacatacctt
    23701 ggaaataaac aattagatag tagtgatgaa aaaattaaat ttataaagaa gttagaagac
    23761 tcagctataa gagaatatat tgattatgga atattgccct ctgtaacaat tggacaagct
    23821 atattagaat ctggctgggg aaattctaaa cttacaaaac agagtaataa tttatttggt
    23881 ataaaagcag ataaagcatg gaaaggaaaa agtgtagaaa tttcaacttc agagcattat
    23941 aatgaaaaaa ttgtagctag ttttaggtcg tataattcat tacaagattc tgtcaaagac
    24001 cacagtttat ttttaattaa taataaaaga tatagaaaac atgggttgtt tgaggcaaaa
    24061 gattatatta gtcaagctca agcattagaa aatgcaggat atagtacagc agaagataaa
    24121 aaaggaaatc gtatatatgc agagctatta atagatatta ttaggagtta taatttgcaa
    24181 ttgatagaca ataaagttga gacaaaataa ttgttaactt tatatgagga gataacaaga
    24241 taagattctt ttctacaaaa agaatagaaa aattgtaaaa aaaactaaaa aaatgtattg
    24301 acttgttatt taaaaggtga tattattatt actgtactcg aaagagaaca aaa
//
LOCUS       DAELSY010000052        24133 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.052, whole genome shotgun sequence.
ACCESSION   DAELSY010000052 DAELSY010000000
VERSION     DAELSY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24133
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.052"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1794
                     /locus_tag="KPG09_001810"
     CDS             <1..1794
                     /locus_tag="KPG09_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0966423.1"
                     /translation="LKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYKSKIN
                     ESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNNRTIG
                     VIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMIEIIE
                     NVEKGVIILDINSKISYINNIALKKLDIYKNIIENIVNIVSVESSSNGHELLEIDIDN
                     KIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNSEAML
                     KVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPENLLE
                     SELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQERKFAR
                     IGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDDIEAI
                     MMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDGIIYK
                     DMLPLDILNYYNINGNVCKNKDINIIFEDDIVGGIVENQERILSIKELELTYINKLLN
                     KYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            1871..2410
                     /locus_tag="KPG09_001811"
     CDS             1871..2410
                     /locus_tag="KPG09_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HBG0966424.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSMYQFQREL
                     YEVLQDFDSNGWEWYHPNRWVPHCTIALTGDDESEAFFRASDVLLREFKKISGEFVSI
                     GLVKITFPVEEIFTVNMNR"
     gene            3033..4247
                     /gene="dpaL"
                     /locus_tag="KPG09_001812"
     CDS             3033..4247
                     /gene="dpaL"
                     /locus_tag="KPG09_001812"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG0966425.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLRDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            4580..5767
                     /locus_tag="KPG09_001813"
     CDS             4580..5767
                     /locus_tag="KPG09_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBG0966426.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYNGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSV
                     YKK"
     gene            5783..7366
                     /locus_tag="KPG09_001814"
     CDS             5783..7366
                     /locus_tag="KPG09_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG0966427.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            7394..8722
                     /gene="ssnA"
                     /locus_tag="KPG09_001815"
     CDS             7394..8722
                     /gene="ssnA"
                     /locus_tag="KPG09_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBG0966428.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            8921..10273
                     /locus_tag="KPG09_001816"
     CDS             8921..10273
                     /locus_tag="KPG09_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG0966429.1"
                     /translation="MSQDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            10465..11574
                     /locus_tag="KPG09_001817"
     CDS             10465..11574
                     /locus_tag="KPG09_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0966430.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            11857..13248
                     /gene="hydA"
                     /locus_tag="KPG09_001818"
     CDS             11857..13248
                     /gene="hydA"
                     /locus_tag="KPG09_001818"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG0966431.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            13242..15803
                     /gene="xdh"
                     /locus_tag="KPG09_001819"
     CDS             13242..15803
                     /gene="xdh"
                     /locus_tag="KPG09_001819"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBG0966432.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            15955..17301
                     /gene="rpoN"
                     /locus_tag="KPG09_001820"
     CDS             15955..17301
                     /gene="rpoN"
                     /locus_tag="KPG09_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBG0966433.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            18098..19120
                     /locus_tag="KPG09_001821"
     CDS             18098..19120
                     /locus_tag="KPG09_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0966434.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            19180..20187
                     /gene="gap"
                     /locus_tag="KPG09_001822"
     CDS             19180..20187
                     /gene="gap"
                     /locus_tag="KPG09_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG0966435.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            20409..21611
                     /locus_tag="KPG09_001823"
     CDS             20409..21611
                     /locus_tag="KPG09_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBG0966436.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKANNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            21637..22380
                     /locus_tag="KPG09_001824"
     CDS             21637..22380
                     /locus_tag="KPG09_001824"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBG0966437.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLSK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            22389..23921
                     /locus_tag="KPG09_001825"
     CDS             22389..23921
                     /locus_tag="KPG09_001825"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBG0966438.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFANKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         9712 a         2736 c         4826 g         6859 t
ORIGIN      
        1 ttaaaagaat ttcaacaaga aatgataaaa tacactgaaa cagtagctag tgtgttagat
       61 gtagatattg aaatagttga tgatagatta ataagaatta gtggtacagg tctgtataaa
      121 tcaaagataa atgaaagcgt tgttacagaa ggatttattt acgacaatgt tatacagaca
      181 ggtcaggaat tagtcgtatt ggatatatgt gataatcagc tttgtataga atgttcccat
      241 tacatgaaat gcctaaataa agtaataata gcagttccaa taaagtataa taatcgaact
      301 ataggagtaa ttggtgctat tagtacagat aaaactaaaa aagtagaaat aagtgcaaaa
      361 atagataatt atttaaaatt tgtcaatcat atatgcgatt taatttctat gaaaatagaa
      421 gaacatgaag cgtcaaaaaa tagcagtaga aaaatggata tgatgataga aatcattgaa
      481 aatgttgaaa aaggcgttat aatactagac ataaatagca aaatatctta tataaacaat
      541 atagctctaa aaaaattaga tatatataaa aatataatag aaaatatagt aaatatagta
      601 tctgttgaat cttcctcaaa tggacatgaa ttactagaaa ttgatattga taacaaaatt
      661 tataatataa atgccaaaat aattccggta tatccatata taaatcaata cgacaaaata
      721 ataatatttg acaagacata tatcaatcat aaaggacatg gaaaagtcaa tagtggctgg
      781 ggaaatagtg atatagagtc tataattgga aattcagagg caatgttaaa agtaaaagag
      841 agaactaaga aattagcaaa gtcaaattca actgtactta taactgggga aagtggtacg
      901 ggtaaagagc taattgctag ggctatacat gctgaaggta gcagatggaa caagccattt
      961 attgcgataa actgtgctgc tattccagaa aatctccttg aaagtgagct atttggctat
     1021 ataaaaggtg catttagtgg agctagtagt ggaggtaaag taggtaaatt tgaacttgca
     1081 aatgaaggtg ttatatttct tgatgaaata ggagatttgt caatgccttt acaagcaaag
     1141 ttacttagag ttttacaaga gagaaaattt gcgagaattg ggtcaaataa acttatagat
     1201 ttagatataa gagtgattgc tgctacaaat aaaaacttat tgaaattagt aaatgaaggt
     1261 aagtttaggg atgatttata ctatagatta aatgtaattc ctataaattt gccacctctt
     1321 agggaaagaa aggatgatat tgaggcaata atgatgaagt ttgcttctaa atattcttta
     1381 gaattaggta ttcagttaaa taaaatagaa gaaaatgtaa tgaatatgtt gattaactat
     1441 aattggccgg gaaatataag ggaactggaa aatgcagttg aatatatgat gaatttggtt
     1501 ggagatgatg gaataatata taaagatatg cttccattgg atattttaaa ttattataat
     1561 attaatggaa atgtttgtaa aaataaggat ataaatataa tatttgaaga tgacatagtt
     1621 ggagggatag tagagaatca ggaaaggata ctttcaatta aagaattaga gttgacttac
     1681 ataaacaagt tgttgaataa gtatggaaga gatacaaaga cgaagaagaa gatagcaaag
     1741 gatttaggta taggtttggc tacactatat agaaaattgg aagaggaaca gtagtaatat
     1801 aatattaaaa tgataggtat ttgtgtgttt ttatagatag gtatttttac tttatggtgg
     1861 aggtgttatt atgcaatatg cgatagagct atattatgat aaaaaaacgg aaaagcaact
     1921 ttttgattta tctaaaaaaa tcgctgatga aaaaattagt acgaagtact tagaatggaa
     1981 aacaagacct catcttactt tggcttgttt caatgatgtg gatgaggctt gttgcataga
     2041 caagctaaag gggtttgcac aaaaccataa ggtaatacct gcttacattg gttcagtagg
     2101 catgtttaat gatactaaaa cggtgtttgt atcacctata atgaatagca gtatgtatca
     2161 gtttcagaga gaattatatg aggtactaca agattttgat tcaaatggtt gggaatggta
     2221 ccaccccaat cgttgggtac cccattgcac aatagcactt actggagatg atgaaagtga
     2281 ggcttttttt cgggcaagtg atgtattact gcgtgagttc aaaaaaataa gtggtgagtt
     2341 tgtctcaata ggattggtta aaattacatt tcctgtggaa gaaattttta cagtgaatat
     2401 gaataggtaa ctttaactct gttgtggatg ttatgaatat tttgcatagt gcatttcatc
     2461 agataaatgc gaagttgcga atttcgacag ctcaatagtc gcaagtagga cacaatggta
     2521 cacatattgc acttttgctt attggaggga cgcccatgta aacttctgta agaagctatg
     2581 aaaagattta agccattttg gaggctttgg gtaataaaga atatttgggt aataaagaat
     2641 ataaagtata taaatttata gagtttatgc gtaagaaggt tatagccata tatttataga
     2701 aaataaaagt agtaatatat ctgacaaaac aatagacata taaaaaataa gagcataata
     2761 aaggaagaat taaaataaat aaacgatttc tcgaattgat aattctcgta ttgataaaaa
     2821 tttcccaata tgagaatttt ttcttttata aatcttattt ttcacattta atatttataa
     2881 aattcaaaga aattttataa atatatgtaa ttccaacata aaaaaattgt ttttagaata
     2941 aataatattg gcatagtaat tgcttcacta taagacacaa agataaaaaa ttaaaatgac
     3001 accgaaaaat ttgaaagggg aatataaaat aaatgagaga aataaaatgg aaattaaata
     3061 acttacctaa atcagaggaa aaagaaaaag gaattgagtt tttaagagat gaagaaattg
     3121 caaaggctaa agcttttcat gagagctttc cacaatatga aaaaactcct ttagttaaat
     3181 tagataactt agcaaaatca ctaggcgtaa gcggaatata tgttaaagac gaatcttata
     3241 gatttggttt aaacgcattt aaagttttag gtggttctta ctctatgggt agatacttag
     3301 ctcaaagatt agatacagat atatctgagt tagggtatga caaattaact tcaaaagaaa
     3361 taaaagaaaa attaggagaa ataactttct ttacagccac agatggaaat catggtagag
     3421 gagttgcttg gacagctaat aaattaggtc aaaaatctgt tgtattaatg ccaaaaggtt
     3481 cttctgaatt tagattaaat aaaatcaaag gagaaggtgc agatgctagt ataacagact
     3541 taaactatga tgatgcagta agattagcta atgattatgc agaagctgat gaccatggag
     3601 taatggttca agatacagca tgggacggat atgaagaaat accagcatgg ataatgcaag
     3661 gatatggaac tatggctaaa gaagctatag aacaattaaa agaatatgga gtagatagac
     3721 caacacacgt atttgttcaa gcaggagttg gttcacttgc tggtgcagtt caagggtatg
     3781 ttgcttcaat atatgatgaa tgcccaataa cagtagtagt agaggctgat gaggctgatt
     3841 gttactataa atcagcagaa gctggagatg gaaaaccaag atttgttgga ggagatatgc
     3901 caactataat ggcagggctt gcttgtggag aaccaaacac tataggtttt gaagtgttaa
     3961 aaaattattc atcagcattc gtttcagcac cagactgggt atctgctaaa ggtatgagaa
     4021 tattaggaaa ccctctaaga ggagatgaga aagtaatctc tggagaatct ggagcagtta
     4081 caactggagc attagtttca atccttgaaa gtgaagattt aaaagactta agagaagctt
     4141 tgaaactaga tgagaactct aaagtattat taataagtac agaaggagat actgaccctg
     4201 ataaatatag agatatagtt tggaatggtg aatgccaaag taaataaaag acttaaggaa
     4261 aataaaaact taacaaataa atgaaaaatt taaggaaaat aaaaacttaa ataagtgaaa
     4321 actttaaata aaataaaaac taaaacaaat gaaaaatcta aataaatgaa aaagtaaagc
     4381 aaatgaaaaa ttcaaaataa aaactaaata aaattaaata aaaccaaata aaattaaaat
     4441 aaagtctaaa taaaaattta ataaataaaa tataaagaaa taaaaaatca atagattaag
     4501 tgaaatgcta ataaattaaa aaagtgaaat atcaaaataa aacgtcaaca aattaaaaat
     4561 tatatggggg aatatcaaaa tgttaaatga aacaagaaaa aaacaattaa ctgaagtatg
     4621 ccaagactta ataagaaatg ctagttattc aggtcaagaa gaaaacgtag taaaagctat
     4681 agaagaaaat tttgctttac taggttttga tagttggtca agagatagat atggaaatat
     4741 agtaggatgt ataaaaggaa ataaaccagg taaaaaaata ttatttgacg ggcatataga
     4801 tacagttcca gttccagatg caagtaaatg gagtgttcct ccttttgaag cgagaatagt
     4861 agatgggaaa atatatggta gaggaacttc tgatatgaag gggcaagtaa gtgctatgat
     4921 ggctgctgtt tcatactttg cagaagatac taacaaagac tttgaaggtg aattgtatgt
     4981 agcaggtgtt gttcatgaag aaatatttga aggtgtttca gctagagaga taagtaaggc
     5041 tgttcaacct gattatgttg taataggtga atcttcagaa ttaaacttaa agataggtca
     5101 aagaggtaga ggagaaatag tagtagaaac ttttggtaaa ccagctcact ctgctaatcc
     5161 agaaaaaggt ataaatgcag tttataaaat ggctaatgta atccaaaaga tacaacaatt
     5221 agtacctcca actcatccag ttttaggtga tggaatatta gttttaacag atataaaatc
     5281 ttctccatat ccaggagcat cagtagtgcc agattattgc aaagctactt ttgatagaag
     5341 attattagtt ggagaaacaa gagaaggcgt attagctcca atacaagctt tattagatga
     5401 aatgatgaaa gaagacccag aattaaatgc aaaagtttct tatgcagttg aaaaagctga
     5461 ttgctataca ggaaatacta tagaaagtga aagattcttc ccaggttggt tatatgatga
     5521 agaagatgaa tttgttcaag cagcgtataa tggattaaaa gaagctggaa tagattcaga
     5581 aataactcaa tactcattct gtacgaatgg aagtcactac gcaggtgagg ctggaataag
     5641 aactatagga tttggtcctt ctaaggaaaa tcttgctcat acaatagatg agtatataga
     5701 attagaccaa ttattcaaag gtgcagaagg atattatggt atattaaaat cagtttataa
     5761 aaaataatta ggagatggtt ttgtgagaac tttaataaaa catgggctta tagtagatgg
     5821 aaataaaaca ccagcatatg aaggagatat tttaatagaa aacgagaaaa tattaaaaat
     5881 atcacaagat ttaaacgaag aagctgacaa agttatagat gcaaaaggta gagttatatg
     5941 tccaggtttc atagatactc atagccattc agacctagtt attttagtta atccatataa
     6001 tgaggtaaag attagacagg gaattacaac agaagtgttg ggccaagatg gtatatctat
     6061 ggcccctcta cctcaagagc atatatcttc ttggagaaaa aatttagcag gtctagatgg
     6121 tgaaagtgat gaaattgatt ggaaatatga aacaacagag aattatttaa aaatgatgga
     6181 ttacaatggg gtaggtttaa atgaaactta tttagtacct catggaaacg ttagaatgga
     6241 agctatggga cttgaagata gacctgctac taaagaagaa attcaaaaaa tgtgtgaaat
     6301 cacagagaga gaattaaaag caggagctat aggattgtct actggactta tatatatacc
     6361 ttgtgcatac tcattgactg aagaaattat agagatgtgt aaggtagtag caaaatatga
     6421 cggagtattt gtagttcacc aaagaagtga agcagataca attttaactt caatggaaga
     6481 aatcatagaa attggaaaac aatcaggagt aaaagttcat ttttcacatt ttaaggtttg
     6541 tggtaaagca aactggaagt atataccaca agttatagag cttttagaaa aagcagaaaa
     6601 ggaaggaata agagtatctt ttgaccaata tccatatgca gcaggaagta caatgcttgg
     6661 agtagtttta cctccttggg cacattctgg aggaactgat aagttaatag aaagattaag
     6721 cgatgaaaac gaaagagcaa aaatgaaaaa agatatagca aatggaatag aaggctggga
     6781 taattttata gaatttgctg gaatagacca aatatttgta acaagcgtaa aaacagaaaa
     6841 gaataaagat acaattggta agagcttatt agaaattgga aaaatgagag gaaaagaccc
     6901 attagatgca acgtttgatt tattaaaaga agaagaaaat gcagttggga tggtagattt
     6961 ctatggatta gaagaacata taataggatt catgaaaaga gacgagcaaa atgtgtgtac
     7021 agatggtctt ttagcaggta aaccacatcc aagagcttat ggttctttcc ctaaaatact
     7081 aggaagatat gttagagagt tgaatgtact aacaattgaa gaagctgttt ataaaatgac
     7141 taaaaaggca gctacaagct ttagtataaa agatagaggc gaattaaaag aaggatattt
     7201 tgcagatata gttatatttg acaaggatac agttagtggt tgtgatgatt ttatcaactc
     7261 tatgcagtac cctacaggta tagattacgt aattataaat ggaaattgtg ttgttgaaga
     7321 aggaaaatac aataaaatta aagcaggaaa agtacttaaa aattaaatct tagctttaag
     7381 gaggaataaa tttatgattt tagtggggaa tgggaggcta ataagccaag attctttaaa
     7441 tccctatatg gaagatggat gtgtagtaat aaatgataat ctaattgaag atataggtac
     7501 tacagaagaa atgaaagcta aatatccagt acatgaattt atagatgcag gcaagaaaat
     7561 aatcatgccg ggtctaataa attcacatat gcatatatac agctcatttg ctagaggaat
     7621 ggcagttcca ggaaaacctt ctgagaactt tttagagata ctaaataatt tatggtggag
     7681 acttgacaaa cagcttacac tggaagatac aaaatacagt gcatatgcaa cgtatataga
     7741 atgcataaaa aatggtgtaa caacagttta tgaccatcat gcaagtccaa atgcaattga
     7801 aggcagtttg tttacaatat cagatgttgc aaaggatttg ggaattagaa cgtgtctttg
     7861 ttatgaagtg tctgatagag atggtggaaa tacaatccaa caaggtatca atgaaaacgt
     7921 aaactttata aaatacgctc aaaaagatga tacagacatg ataaaaggaa tgtttgggct
     7981 tcatgctgca tttacactat ctgatgatac attaaataaa tgtgcttcag agatggctgg
     8041 tcttgataca ggttaccatg tacatgttgc tgaaggacta gcagacttac aatataatgt
     8101 agataaatat ggaaaaaggg ttttagaaag attcaatgat tttggagtac taaaagaaaa
     8161 aacaatagca gttcattgta tatatataag tgcagaagaa atcagtcttt taaataaaac
     8221 aaatacattt gtagttcata atccagaatc taatatggga aatgcagttg gatgttctcc
     8281 tgcaatagag ttattaaaaa ggggagttac agtaggatta ggaactgatg gatatactag
     8341 cgatatgttt gaatcattaa aagttgccaa tataattcac aaacatcact taaaagaccc
     8401 aagagttgga tttgaagaat cacaaacaat gttatttgat aacaatagaa aaatagctaa
     8461 agcttattat aaaaatgacc taggagtact taaaaaaggt gcttatgcag atgttatagt
     8521 agttgattat ataccacata ctccattgac agaaaataca atagggggac acactatttt
     8581 tggaatgagt ggaagaagcg tagatacaac tattataaat ggaaaagtac ttatgaaaga
     8641 tagaaagtta ataaacatag atgaagaaag tattctagca aaaagtagag aattaagtca
     8701 aaatctatgg aatagaattt aaataattga ttatgcaaaa ttctatgaag caggaaccct
     8761 gtgactttag tcatgggagg ttcagattaa tatacaagta agaaacaaga atttagatgt
     8821 taaaaacaga gcttaaacaa aaataggaat ttaaatgaag tatataaata aaaaatatac
     8881 ggaatagaat attaaattaa atatagaagg gagacccaaa atgtctcaag ataaaagccc
     8941 taaaatagct ccagtagatg aaaggttacc tgtatccaaa tcttgggtat tttcactaca
     9001 gcacgtttta gcaatgtgtg caggagcagt agcagttcct atgatgatag gaagtgctgc
     9061 acgactagat catgctagta ttgttttttt aataaatgct tgcttgttta tggcaggtat
     9121 aggaacactc atacaaagtg ttggtatagg aaatattgta ggtgctaaaa taccagtaat
     9181 agaaggaact agttttgccg ctgtggcagg tatgacagca atagcctcat catatagtga
     9241 ttcacaagtt gcaatacaga cagtatttgg ctctgtaata gtagctggat tgttttgtat
     9301 attacttgct ccagtatttg aaaaattaat aagatttttc cctagtgtag ttactggaac
     9361 agttgttatg gtaataggta tttcactatt tccagttggg ataaaatgga taactgacaa
     9421 caaattggaa gcggttgaac caaactcatt agcattagct ttagcagtat tggcaataac
     9481 tttactttca tttaaattct taaaaggaat ttggaatagc acagctatat tggtaggtat
     9541 agtacttggt acactacttt ccatggcagt tggaatggca gattttagtg cagtaaatgg
     9601 agttggttgg cttaacttaa atgtaccttt aaaatttgga atgcctagat ttgaaccatc
     9661 tgcaatacta tcattgtgtt tgattatgtt ggtacttatg acggaatcag ttggtaatat
     9721 gatagctatt catgaaatgg tagacaaaga agtaacaggt aaaaatatta aaagaggtct
     9781 atttggagat ggaatatcta cagcattatc tggaatattc aattcatttc caataactcc
     9841 atttggtcaa aatacaggaa tagtaggtct tacaggtata aaatcaagat ttatagcagt
     9901 ttacgcaggg gttatattgc tagtacttgg attcttacca aaatttgcag caataatagg
     9961 agcaatacct aagcctgttc tcggaggcgt aggatttgca atgtttggaa tggtaatggt
    10021 tggaggagta aaaactttaa gcaaagtgaa atttgatgga actaaaaatg gggttatagt
    10081 agcagttagt gttggtttgt ctatgatacc actaacaaat cccgatttct atcataattt
    10141 cccaacttgg gtacaaacaa tattccatag tggaataaca acaggtagtt tatcagcagt
    10201 gcttttaaac atattcttca atgaactagg ttctaaaaaa gaaaaaaata ataaagttga
    10261 agaagcaaaa taaaaataaa acaagtaaaa atctatattg acttaaagtc attaagcatt
    10321 aataaaaata attcagagat attaataatt caacaaaact atcttatatt ttaaataata
    10381 taaaaaagta aataaataac gaatacaaat aaaagaatac aatctaaata ttaaaaatat
    10441 ttaatttaag ggagagataa taatatggct aatttaaaag ttaatttata tggaatggaa
    10501 tttttaaacc caattatgac agcggcagga cctggagcaa aagatggtgc tctctgtcaa
    10561 gaggcagcaa aaggtggagc aggagctata tgtacaaaaa ctatatctgt attaccagct
    10621 gatgtaccaa gaccatgtat ggcaaaaact aatagtggtt ttttaaacac agaattatgg
    10681 tcagagcttc caaaagagca atggatagag gaagaataca aaaaggtaaa agaaacaggg
    10741 cttccagtaa tagtaagtat gggatattca gcagagcaaa tagctgaggt tgctccttta
    10801 gtaaagcctt atgcagatgc agttgaatta tctactcatt atgtaggtac agacgttgca
    10861 ccaatagtaa atgctttaaa agctgcaaaa gctgttttag atgtaccagt atttatgaaa
    10921 atgagtcctc atactgacat tcaaactatt gcaaaagctg ttgaagaagc aggtgcagat
    10981 ggacttgtaa tgataaactc atttggacca tgtatggcaa tagatgtaaa tacaggatac
    11041 cctataatgg gaagtaaagc tggatacgga tggttatcag gtgcaccaat aagacctcta
    11101 gcaataagat gtatatatga agcttctcaa gttgtaaata taccaataat cggtgttggt
    11161 ggaatcactt gtggaaaaga cgtggctgaa atgatgatgg ctggagcaag tgctgttcaa
    11221 gtatgtacag aagcaatcct taaagggcct tctatctatg gaaaaattgc taaagaatta
    11281 aatgaatttt tagatgagaa tggatatgct gatgttaatg aaataaaagg attaactcat
    11341 aagaaaatgg cagctagagg gtttagaact cattcagtag ctccagtagt ggatgataac
    11401 aagtgcatca aatgtaagca atgtgtaaca agctgtgtat acgaagcttt agaagttaca
    11461 gataagttga acatagatgc agacaagtgt tttggatgtg gattatgtgt aactagatgt
    11521 ccaaaaggag ctttaagcat ctgctacaat caagtagaag ctatgaatga gtaataatta
    11581 tatagaattt tggtaattaa cttagagtaa taggtattag gataataaaa tatatcagat
    11641 aaacaaacac acattatgca ttaataatac agtctaaagt taataatttt attatattga
    11701 tttaactaaa tattagttta gctgaatatt aatttaatta ggtattggtt taatcttaat
    11761 atagatttac tacgtataag tgaactaact agattacttt aagttaattt tttcatataa
    11821 aataaattga tacattacaa gtaaggtgga gtctatatgg gaattgtttt aaaaggtggg
    11881 aaagtagtaa caagtgaaaa aacttactgt gcagatgtaa gaatagaaga tgaaaaaata
    11941 gtcgatatag gtcttgatat atcaaaagaa aatgatgaaa tagttgatgt tagtgattct
    12001 tatgttatac caggaggtat agatacacac acacactttg acttagatac aggcgttact
    12061 aagacagcag ataactttga gacaggaaca aaagctgcta tagtaggagg aactactact
    12121 atattggatt ttgctacaca agctaaagga tgttctttga aagacggatt agaagaatgg
    12181 catgaaaaag ctaaggataa atcctattca gattatggct ttcatatggc tataacagat
    12241 tggaatgact cggtttgtaa tgaaatggag gatatggtga aagagggagt atcttcattt
    12301 aaactgtata tggcgtataa aggaagcctt caagttgatg atggtgtaat ctttgaagca
    12361 ttgagaaaag cagaagagat aggagggatt attggtttcc attgtgaaaa tggggacata
    12421 atatgtgaac ttgtggataa agcaaagtca gaaaataatt tatctccaaa atatcatcaa
    12481 ttaactagac cagctataat ggaggcagaa gctacatcaa gacttatgaa gatagctgaa
    12541 gttgcaaact caaaagtata tgtagttcac ttaagctgta aagaatcctt agaggaggtt
    12601 ttaaaagcta agcaaagagg tgtggatgta atagttgaaa cttgtcctca ataccttcta
    12661 ttaaatgatg aattatatga tttagatgga tttgaaagtg ctaagtatgt aatgtcacca
    12721 cctttaagaa ataaagaaaa taatgaaata ttatggaatg ctattttaga taatcaaata
    12781 gacactatag gaacagacca ttgttctttt aattataagg gacagaagga acttgggata
    12841 aacgatttca gtaagatacc aaatggttct ccaggtgtag aacataggat gagtctttta
    12901 tacacatatg gagtctgtaa aaataaaata tctatcaaca agatggtaga agtaacatca
    12961 actaatgcag caaagttgtt tggaatgtat cctaataaag gaactataga aataggcagt
    13021 gatgcagata tattagtctt aaatccaaac aaaaaatcca aaatagaagc aaaaaaacaa
    13081 ttacaaaatg tagattatac accttatgaa gggtatgaag tggattgtca aatagaacat
    13141 gtttatttaa gaggaaaaga agtagttaga gatggagaat taatttgtaa tactccaagt
    13201 gggagatata taaataggcg tctaacaaag ggggctagag aatggtagaa tttatattaa
    13261 atggagagaa agttgttgca aaagaaggca aaaaactttt agacttcctt agagaagata
    13321 tgaatataac ttcagttaaa aatggctgtt cagaaggtgc ttgtggaaca tgtatggtta
    13381 taatagatgg taaagctact aaagcatgtg tatttaaaac tgataaatta gctgggaaaa
    13441 acataacaac tatagaggga ttatcagaga gagaaagaga tgtttttgca tattcttttg
    13501 ctagtcaagg agctgtacaa tgtggatttt gtattccagg tatggtaatt agttcaaaag
    13561 cacttataga tgttaataat aatcctactg aagatgatat aaagaaagct ttaaaaggta
    13621 atatatgtag atgtacaggg tatgtaaaaa taattaaagc tgtcgattta gcagcaaaga
    13681 tttttagaga aaatatagat gtgccaaaag taaactgtaa gggtctagta ggtgaaaatc
    13741 ttcctagagt tgatgcagtt ggcaaagcac ttggatattc tgaatatgta gatgatatga
    13801 gaattgaggg tatgatttat ggttcagcag ttagagctaa gtatcctaga gctttagtta
    13861 aaaaaataga tatagaagaa gcaaaagctt atccaggagt tgttgcagtt ctaacagcta
    13921 aagatattcc aggaactata aaggtagggc acttaaagaa ggattgggat actttaatac
    13981 cagaaggtga aataactaga tatttaggag atggaatagt tttagtagca gcagaaacta
    14041 gagaagcgtt agaagaggcc aaaaagctag ttaagataga ttatgaggaa ttaacaccat
    14101 taagtactac agcagatgca cttagagaag atgctccaaa gatacatgag acagggaata
    14161 tacttgttaa ggaacattta gtaagaggaa acgctaaaga aaagatagaa aattcaaaat
    14221 atgttgtaac taaaagatat actacacctg ctacagaaca cgcattttta gagcctgaat
    14281 gtgcagtagc tggtccttat gaagatgatg gagtaataat ctattcaaca gaccaagggg
    14341 tgtttgccac tcaacatgaa tgtgcagata tgttagggtt accatatgag aaggtaagag
    14401 ttataaacaa aatggtagga ggagggtttg gtggaaaaga agacatgagt gttcaacacc
    14461 atgcagcctt acttgcatac catacaaaga gatttgtaaa agtacagtta acaagaaaag
    14521 aaagtattat tgttcatcca aaacgacatg ccacagaaat ggaatttacg acagcatgtg
    14581 atgaaaatgg atacttaacg ggtatgaagg caacgataat atctgacaca ggtgcatatg
    14641 cctctcttgg aggaccagtg ctacaaagac tgtgtactca tgcagcagga ccatacaatt
    14701 atcaagatat tgatataaca ggaacagcag tatatactaa taaccctcct ggaggagcat
    14761 ttagaggatt tggagttaca cagagttgtt ttgcaactga atgtaatcta aatttacttg
    14821 ctgaaatggt tggaatatct ccttgggaga taagatataa aaatgcaata agacctgggc
    14881 aagttttacc aaatggtcaa tttgcagatg aaggaacagc aatggcagag acactagaag
    14941 ctgtaaaaga agagtatgaa aagcatccac gtgcaggtat agcttgtgct atgaaaaata
    15001 gtggggttgg agttggtata cctgatgttg gaagatgtag attggtgata aaagatggca
    15061 aagttcacat aagaacaagt gcttcttgta taggtcaagg gcttggtact gttatgactc
    15121 aagtagtttg tgaaactgct agcctacttc ctgaaaatat agtgcatgaa gttccagata
    15181 ctcatttaac accagactct ggtaatacta cggcttcaag acagacagta tttacgggag
    15241 aagctactaa ggttgcatca aataagttaa aagaagcttt agactctggc aaaactttac
    15301 ttgatttaga gggagaagag ttttatggag aatatgaaag tattacagat aaaatgggtt
    15361 gtgacaaaga aaatccagta agtcatgttg cttatggata tgcaacacaa gttgtaattc
    15421 tggatgaaaa tggaaaacta gaaaaagtag tagcagcaca tgatgttgga agagctataa
    15481 atccaatcag tttagaaggt caaatagaag gtggagtagt tatgggtctt ggatatgctc
    15541 taaccgaaga ttacccagta gttaactctg ttcctacagc taagtttggt acattaggtt
    15601 tatttaaatc tacagatata ccagaaatac aagctttaat agttgaaaag aacaaagatt
    15661 tattggctta tggggcaaaa ggtgtaggag aaatttgtac agttcctact gcaccagcag
    15721 tacaaaatgc ctattatgta tttgataaag aatttagaac tgagctacca ctacaaaata
    15781 ctccatatag taaaaaaaag taacaaaaaa aagaaatatt aaataaaaaa atagatgaaa
    15841 ttgtaaataa aaaaaaccct tttgcataag gtatatttta aatgatagaa tatacatata
    15901 caggagggtt tttcttttat aaaataagtt taataaaatt gagaggtgag cagaatgaat
    15961 tttaataata gtctagaatt aactcagtct cagaagctaa taatgactac acaacttaag
    16021 caatcattat caatattaaa tatgagtaag ttagagttag aagaagagat aaaaagagaa
    16081 gcagagaata acccattagt tgaagtcgag aaaagtgggg agataaattg ggaagagtat
    16141 ataaaagaca tggataggtc aagacgttta gatagaacag agataagcta taatcctgat
    16201 aatgaagtta atctagaaaa tttagttaaa tattcgtcaa atttatatga agatttaaaa
    16261 tttcagataa gcctgtataa acttacagac aaagaattgg aagtctgtga gtatataatt
    16321 gatagtttag atgaggatgg atatttaaga actgaggaaa aagtaattgt tgatacctta
    16381 aagatagatg aagaactttt tgaaaaatgt ttaattagtg ttcagcagtt ggaacctagt
    16441 ggagtagggg caagaagtct ttcagaatgt ctgatgatac aaatggctag tttaggaata
    16501 tataatgaaa ttttagagga aatcgttaca aaagacttaa atttaattgc aaataataaa
    16561 tataaagaga taagtaaaaa gtacaatatg tcattacaaa aatgtgttga actaataaac
    16621 ataattaaga cattagaccc caaaccagga agaacttgtt cagtagagaa aagtgtatac
    16681 attcaacctg atgtaacagt agaaaagatt gatgatgaat ttattgtata tttaaatgag
    16741 aaagattctt accaaataag aatcaatagt tattataaag acatattaaa aaattcacag
    16801 tctgatgaaa gtgcaaaaga atttataaaa gaaagactaa attcagcaac aggacttatg
    16861 aaaaatatag agagtaggaa aactacagta cttaagattg cagaagagat agtaaaagct
    16921 caagatgaat ttttgagaaa agggacaaaa tatataaaac ctttgaaaat gaaagacata
    16981 gcagaaaaat tagaatttca tgaatctact ataagtagag gtgtaaatgg taaatatatg
    17041 ctaacacctt ttggcgtata tgaatttagg tactttttca gtagcgccat tgagactgag
    17101 aataatgaaa tggcatctag tacaagcata aaaaaaatta taaaggaaac aataaaagac
    17161 gaaaataaaa agaaaccttt aagtgatgac catatatcta agttattaaa agaaaaagga
    17221 atcaatgttg caagaaggac agttgccaaa tatcgtgaag aattaggaat cccatcttca
    17281 agtaagagaa aagaatacta ataagtaaaa gacaaatatc agtatggtaa ggggaaaagg
    17341 ctaattaaga gatgaatgta gattgggcta aaaattttat aaagtaaaat aatagaatat
    17401 ggaaaagtat taaaatgcta taaggtatta aaaaactggg gaacattaaa acattaagat
    17461 agcaaagaac ataaaaaact aaagaagatt aaaacattaa agaacattag gctttgagga
    17521 atattagaac tttaaagaat attaaaattt ataaaatata aaaatactaa aaagaattga
    17581 aaaactgggg aacattaaaa tattgtaaaa tgctaaaaaa ctagaaaaca taattaaata
    17641 agtgcttata aagaatgatt aaattaaata agtgtttgta aagaataatt agatattaga
    17701 aaaagaagaa tacctagata aataaactat tattaaattt aataatcaca aaaggaaaac
    17761 acgtagggta taaataaaaa tcttaactag ataaaagtaa acttagttgt agataaaaat
    17821 aactagaaat aaaagtaact agaaataaaa ttatttatag taataaatat tagaatgaaa
    17881 atataaaaaa atatatataa aaagccttat ataaaaagaa aaaatactgt gatttgaaaa
    17941 tggtggagaa atattttata aaataaaaaa ataaaaaagt ttttgaaaaa ctattgaaat
    18001 ctatacgcaa atcctataaa ataaaaatat cttgtgggac ttaaataaac actagggaca
    18061 aaaaaagtcc cgaaaggaaa gcaataagga gtatgttatg aagaacttat tagaaattca
    18121 aaagaaattg attccacagg caattgaact tatggaaaga agatactcta ttttaagaca
    18181 aatatcttta agtgaaccaa taggtaggag aacattatca aatgtattag agattagtga
    18241 aagaataatc aggtcagaaa ctgagttttt aaaagaacaa ggacttatag acgtagctgt
    18301 atctgggatg actatcacta aagaaggttc tgatttgtta gataaactta aggacatcat
    18361 gagtgacatc atgggattgt ctaagttaca agatagagtc agagaaaagc ttggaataaa
    18421 aagggtaata ctagttcctg gaagctgtga tgaaaatgga agtttactaa aagatgtaac
    18481 tagatatgga tgcgagtatt tcttaggcat tttaaaagat ggagatatcg tttcaataac
    18541 aggaggaagc actatgttgg agttttcaaa cgttattaaa actgataaga gatatccaaa
    18601 ctctacaata gttccagcta gaggaagtat gggaactgat gtagagatgc aatctaacaa
    18661 tatagttgct tcaataagta agaaattaca ttctaattgc aaacttttac atataccaga
    18721 tgagttggat gaaagtgcaa tgaaaactct tagccaagtg ccagagatta agacaacttt
    18781 ggagtatatt aaaagaacag acattttagt ttttagtata ggtagagcag atgtcatggc
    18841 aaagagaaga aaacttccag aagacaaggt tgatgagata ttatctaaaa atgcagttgg
    18901 agaagcattt ggatattatt tcaacaaaga tggagaaata gtttacaaac tgaacactgt
    18961 tggaattgat ttagaaacag ttaaacaagt aaaagaaact attgcaatct ttgcagggac
    19021 tgaaaaagta gaagccttat tagctatatc aaatatgcat aaaaacatgg ttcttgtaac
    19081 agatgaagaa agtgcttata agatattgtc attattataa aagtcaagac ctagcacaga
    19141 gctagaaaac atacatttac tttataggag gtagcgaaaa tggttaaagt agctataaat
    19201 ggatttggaa gaataggaag attagcatta agaaaaatga tggagcaaca agataagttc
    19261 gaagttgttg caataaacga cttaacagac gctaaaatgt tagctcattt atttaaatat
    19321 gatacagctc aaggaagatt taacggagaa atagaagtta aagaaggtgc ttttgtagta
    19381 aacggaaaag aaataaaagt aacagcagaa agaaatcctg ctgacctacc atgggctgaa
    19441 ttaggagtag atatagtatt agaatgtaca ggattcttta catctaaaga taaagctgaa
    19501 gctcatatac aagcaggggc taagaaagtt gttatatctg caccagctac tggagattta
    19561 aaaacaatag tattcaacac aaacagtgac atattagatg gttctgaaac agttatatca
    19621 ggagcttctt gtactactaa ctgtttagct ccaatggcta aagtattaaa tgataaatac
    19681 ggtatagaaa aaggattaat gactactata catgcataca ctaatgacca aaatacttta
    19741 gatggtcctc atccaaaagg tgatttaaga agagctagag ctgctgctgg aaacatagtt
    19801 ccaaacacta caggagctgc aaaagcaata ggcttagtaa taccttcatt aaaaggaaaa
    19861 ttagatgggg cagcacaaag agtaccagtt gtaactggtt caataactga attagtttgt
    19921 acattaggaa aaaatgttac agttgaagaa ataaatgcag ctatgaaaga agcttcaaac
    19981 gaatctttcg gatatactga agaaatgtta gtatcttctg acataatcgg aataagctac
    20041 ggttcattat ttgacgctac tcaaacaaaa gttatggaag tagatggaaa acaattagtt
    20101 aaagttgttt cttggtatga taatgaaatg tcttatactt ctcaattaat aagaacttta
    20161 ggatactttg cacaattagc aaaatagtca gttttgagaa aatagaaaag tagattgaca
    20221 aagtccgagt ttgtagttta atactacgga ctcaggactt tttttaagaa aaaatatata
    20281 aaatacacct aaataatgtt acatactata tataaaaata tgataatatg atatatttaa
    20341 tagggtaaaa gtatgtacta tattatactt ggcaataaaa aattacttta ttaaaaggag
    20401 agatatttat gtcaatgctt aacaaaaaaa ctatagaaga tattgatgtg tgcggaaaaa
    20461 aagtattagt aagatgtgac tttaatgtgc cattacaaga tggtgtaatt actgatgaaa
    20521 atagattgaa cggagcttta ccaactattc aatatttaat ttctaaaggg gcaaaagtta
    20581 tattatgctc tcacttagga aagccaaaag gagaagctaa accagaatta tctttagcac
    20641 cagtagcaaa gagattatct gagatgttag gaaaagaagt tgtatttgca gcagacgata
    20701 atgttgtagg agaaaatgca aagaaagcaa ctgaaaaaat ggaaaacgga gatgtagtat
    20761 tattagaaaa tactagatat agaaaagaag aaactaaaaa cgaagagaat ttctctaaag
    20821 aactagcatc acttgctgaa atatttgtaa atgatgcatt tggaactgct catagagcac
    20881 attgctcaac agttggtgca ggcgagtttt tacaagagag agtttgtgga tacttaattc
    20941 aaaaagaatt aaaattctta ggagaagctg ttgcaaaccc tgtaagacca tttacagcta
    21001 tacttggagg agctaaagtt tctgataaat tagctgttat aaacgaatta ttagaaaaag
    21061 ttgataatct aataataggt ggaggaatgg cttatacatt cttaaaagct cagggttatg
    21121 aagtaggaac ttctttactt gaaatagata aagttgaata tgctaaagaa atgatggaaa
    21181 aagctaataa taagggtgtt aacttattat taccagtaga cgttgttatg gcagaccact
    21241 ttgcaccaga tgctactcca atagtaacag aagatgctaa tgtaaaagaa gattacatgg
    21301 gactagatat gggacctaag actatagcaa actttgtaaa aactataaaa gaatcaaaaa
    21361 ctgtagtatg gaatgggcca atgggagtat ttgaatttga aaactttgca aatggtacat
    21421 tatcagtagc tagagctatg gcagagttga ctgatgcaac tactgtaatt ggcggtggag
    21481 atagtgcagc agcagttaat caattaggat ttggtgacaa gatgactcac gtatcaacag
    21541 gtggaggagc atcactagaa tttttagaag gtaaagaatt accaggtatt gcagcattag
    21601 ataataaata aactaatata tagaggagat tgtaaaatga gaaaacctat aattgcagga
    21661 aactggaaaa tgcataaaac aataaaagaa gctttagaat ttgtaaatga agttaaagat
    21721 aaagtaaatt ctgataaagt agaagctgtt atatgtgcac catttacttt attaaaggat
    21781 ttaaaagaag ctactaaggg tacaaatata aagataggtg ctcaaaatat gcactttgaa
    21841 gaaaaaggag catttacagg agaagtttca cctcttatgt taaaagaaat agatatggac
    21901 tatgttgtaa taggacattc agaaagaaga caatacttca atgaaactga tgaaactgta
    21961 aacaagaaag ttttaaaagc attagaagta ggaatagacc caatattatg tgttggagaa
    22021 actttagaac aaagagaagc tggaaaaact aaagatgttt gtaaagttca agttgaaaaa
    22081 gctttagaaa atgttcttaa agatgattta tcaaaagtag ttgttgcata tgagccaatc
    22141 tgggctattg gaactggtaa aactgcaact gctgaagatg ctaatgacgt tatatcttat
    22201 ataagagaag ttataaaagg attatatgga gaattagcta atgaagttag aatacaatac
    22261 ggtggaagtg ttaaaccttc aaatgtagcc gaaataatgg gtcaaagtga tatagatgga
    22321 gctttagtag gtggagctag tttagctagt aatgattacc tagaccttgt taatttctaa
    22381 ggagtgaaat gatgaaaaaa ccagttgctt taattattat ggacgggttt ggatacaata
    22441 aggatgttaa gggaaatgca attgctgagt ccaaaactcc taatttagat agaataaaaa
    22501 aagagtatcc aaacacattg ataaatgcta gtggtcttga tgttggtcta cctgatggtc
    22561 aaatgggaaa ttcagaagtt ggacacacta acataggtgc aggtagaata gtatatcaag
    22621 atttaactag aataacaaaa tctataaaag atggtgactt ttttgctaat aaagttttat
    22681 gtgaagcaat ggataatgca aaagaaaatt ctcttcatgt aatgggactt ttgtctgatg
    22741 gaggagtaca ttcacatata gaccatctta aggctataat caaaatggca aaagataaag
    22801 gcgtacaaaa ggtgtatgtt cacgcattta ctgatggtag agatactgac cctcaaagtg
    22861 cattagaata tgccaaagaa gtacaagcta gtatggatga aattggagta ggtgaatttg
    22921 ctacagtttc tggtagatac tacgctatgg atagagataa gagatgggaa agagtagaac
    22981 ttgcatacaa tgccatggtt agaggtattg gtgaaaaagc aaattctatt gaggaagcta
    23041 ttcaaaactc ttatgatgat ggaaaaaatg atgagtttat aatgccaaca gttattatga
    23101 aagatgataa accagttgga agtataaagg aaaatgattc aattatattc ttcaacttta
    23161 gaccagatag agctagacaa ataactagag ctttagtttg tgaagagttt gatggattta
    23221 aaagagaaga tattaaaaac ttctttgtat gcttaacaga gtacgatata actatagaga
    23281 atgtgcatat agcatttgga cctcaatctt tagccaacac tttaggtgag tatctagcta
    23341 aaaatggaaa aactcaactt agagctgctg aaactgaaaa gtatgcacat gtaactttct
    23401 tcttcaatgg tggagtagaa gagccaaata aaggtgaaga aagactattg ataccttctc
    23461 caaaggttgc aacttatgat ttaaaaccag aaatgtcagc atatgaattg acagataaag
    23521 ctttagataa attaggagaa gataagtttg actttatagt attaaacttt gcaaatcctg
    23581 atatggtagg tcacacagga agtatagaag cggctataaa ggctgttgaa actgtagata
    23641 cttgtgtagg aaaattgata gataaaatag tagagttagg tggaagtgca ataataactg
    23701 ctgaccacgg aaatgctgaa tatatgttag acccggaaac aggaaaaact gtaacggctc
    23761 actctattaa tccagtacca tttattgtag ttggtcaaga atatgaaagt gcaaaattat
    23821 tggatggagg aagattatct gatatagcac cgactgtttt agatatgatg aagttagaaa
    23881 agccagaaga aatgactggt cattcactta tttcaaaata gtcgattctg ataagtagag
    23941 aataatatat aaactacaat ataaatttaa aaataatgca gtttaggttt attttgtaaa
    24001 gtttagactt tatgacataa gatttagatt ttgtaagttc tggatattgt aaggtgtaga
    24061 tgaaggtata ttttataaaa tttacaagat agacaattta taaaataaaa aattataata
    24121 tagataataa tgt
//
LOCUS       DAELSY010000053        24128 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.053, whole genome shotgun sequence.
ACCESSION   DAELSY010000053 DAELSY010000000
VERSION     DAELSY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24128
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.053"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      228..329
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            402..1745
                     /locus_tag="KPG09_001826"
     CDS             402..1745
                     /locus_tag="KPG09_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG0966439.1"
                     /translation="MVPNTNNVQPDGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     gene            1892..3256
                     /locus_tag="KPG09_001827"
     CDS             1892..3256
                     /locus_tag="KPG09_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0966440.1"
                     /translation="MSQLLIKNAYLITMNADREVYETGSILIENNIIKNVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLIRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHNPGAAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(3434..5299)
                     /locus_tag="KPG09_001828"
     CDS             complement(3434..5299)
                     /locus_tag="KPG09_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBG0966441.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIEYNKIEFEKEINDILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(5535..6752)
                     /locus_tag="KPG09_001829"
     CDS             complement(5535..6752)
                     /locus_tag="KPG09_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0966442.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAILIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLIVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            complement(6952..7815)
                     /gene="aroE"
                     /locus_tag="KPG09_001830"
     CDS             complement(6952..7815)
                     /gene="aroE"
                     /locus_tag="KPG09_001830"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG0966443.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGIEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNAKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIESSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            complement(7897..9819)
                     /locus_tag="KPG09_001831"
     CDS             complement(7897..9819)
                     /locus_tag="KPG09_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG0966444.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDIHDSSVIAAYLEGCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            complement(9898..10746)
                     /locus_tag="KPG09_001832"
     CDS             complement(9898..10746)
                     /locus_tag="KPG09_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBG0966445.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGTEEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            11159..12040
                     /locus_tag="KPG09_001833"
     CDS             11159..12040
                     /locus_tag="KPG09_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0966446.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(12248..13111)
                     /locus_tag="KPG09_001834"
     CDS             complement(12248..13111)
                     /locus_tag="KPG09_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBG0966447.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(13266..15200)
                     /locus_tag="KPG09_001835"
     CDS             complement(13266..15200)
                     /locus_tag="KPG09_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBG0966448.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKAINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLSV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSLEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(15312..16325)
                     /gene="galE"
                     /locus_tag="KPG09_001836"
     CDS             complement(15312..16325)
                     /gene="galE"
                     /locus_tag="KPG09_001836"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBG0966449.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(16350..17219)
                     /gene="galU"
                     /locus_tag="KPG09_001837"
     CDS             complement(16350..17219)
                     /gene="galU"
                     /locus_tag="KPG09_001837"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG0966450.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(17417..17737)
                     /locus_tag="KPG09_001838"
     CDS             complement(17417..17737)
                     /locus_tag="KPG09_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966451.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(17727..19718)
                     /locus_tag="KPG09_001839"
     CDS             complement(17727..19718)
                     /locus_tag="KPG09_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966452.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCDIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKNMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(19840..22383)
                     /locus_tag="KPG09_001840"
     CDS             complement(19840..22383)
                     /locus_tag="KPG09_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBG0966453.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGAKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQAKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(22661..22995)
                     /gene="rnpB"
                     /locus_tag="KPG09_001841"
     ncRNA           complement(22661..22995)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KPG09_001841"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            23127..23804
                     /gene="srtB"
                     /locus_tag="KPG09_001842"
     CDS             23127..23804
                     /gene="srtB"
                     /locus_tag="KPG09_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBG0966454.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKTSSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKNNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(23964..24038)
                     /locus_tag="KPG09_001843"
     tRNA            complement(23964..24038)
                     /locus_tag="KPG09_001843"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24004..24006),aa:Thr,seq:tgt)
BASE COUNT         7725 a         4171 c         2709 g         9523 t
ORIGIN      
        1 gtgtattcag tacttttata attatatcac aaactcattt ataatagact tctatataat
       61 agtataaaac aagttagaat aattgacatt atacttatac atattcaatt attgaaaaca
      121 gtaattcttg ccaataaata accttaattt tttataaaat cgacaaaaaa acgaataatt
      181 ttattatatt tattgtaaat aatacaatat tacgatataa tacaacagaa aaacaaatag
      241 aaaatgttca tataatatta gtaatacggt ctaatagttt ctacaaactc accttaaatg
      301 ggttttctat gaatagaata tcaactttga atatatgttc tattctatct atatgaatat
      361 atattttttt gtttatatct taaagttttt tggaggtaaa aatggtacca aacactaaca
      421 atgtacaacc agatggaaat aattcatcat taagcttttt agacaaaatg tttaaattta
      481 gcgaacgtgg ctctagcctt aaaacagaag taataggtgg agttactaca ttcttaacaa
      541 tggcatacat agttttcgta aatccagcaa tattatctga agctggaatg gataaagccg
      601 cccttataac agttaccata ctagctacag ccattggtac acttatattt gcgattttag
      661 gaaatgcacc atttgctcta gctcctggta tgggattaaa tgcatttttc acatactctt
      721 tggttatagg tgcaggaatc ccatggcaaa caggtcttgg ggtagttttt ctatctggat
      781 gtgtcttctt tacactcgca gtaacaggtc ttcgaaagac aattgcagac gcaattccta
      841 gagaattggc agtagcatct tcagcaggta taggtctttt ccttgctttt gttggtttaa
      901 aaaatatggg tgttataata gctaatccag atactatagt ttcacttgca gattttacac
      961 caacagtttt aatagctctt tttgctctta tcttaatggc aatactagaa tataaaaaag
     1021 ttaaaggtgg aatattgata agtatagtag ttgcaacaat aatatctgtt atatttggat
     1081 ttgtggagtt gccaacagca ataatctcaa cacctcctag tatagcacca gttgctttta
     1141 aattagatat tgtaggtgca ttgaaattct cattaattgg acctatattc tctttcatgt
     1201 ttatagacat gtttgattca ttagcatttc ttattacttg ttgtaaacaa atgggtcttg
     1261 aagatgagaa tggtgaaata aaagatttag gaagaatgtt atatgcagat gtttcttcta
     1321 ctttaatagg ctcattctta ggaactagta ctgttacaac atttggagaa tctgcttctg
     1381 gtatcgcagc aggtgcaaga acaggacttg cttcaatagt aacagcagca ttattcttag
     1441 ctacacttat atttacacca ttgcttggta tagttcctac ttacgctgca gctcctgcac
     1501 ttgtaatggt tggtgtattc atgtttgaaa gcataaaaca gattgatttt gctgatagca
     1561 aaatagctgt acctgctttt gttacagttt tattgatgcc tttaacttat agtataagta
     1621 ttggtttatg ctttggattt atttcatata taataatgca tgtggtagct aaagaaactg
     1681 ataaaatcag tattacactt tggattatag gtttacttgc agttgtaaac ttagttctaa
     1741 gttaatattt taaatagttc taggtcaata ttttaaattt ataaatagca aaatcaattt
     1801 atgtataaat tgattttgct attaactttg tatagaaaat ttattatcat aatacataga
     1861 tataaaaata tatagaaaag aggttgtact tatgtcacaa ttacttataa aaaatgcata
     1921 tctaattact atgaatgcag atagagaggt atatgaaact ggaagtatac ttattgaaaa
     1981 taatattata aaaaatgttg gtaagtttga tattaatatt gtagataaag atgctgagat
     2041 atatgatgct aaaggaaaaa tactaatgcc agggttagtc aatacacacg ttcacttatc
     2101 ccaacaatta ggacgtggtc ttgcagatga tgttgtactt ttaacttggc ttagagagag
     2161 aatctggcca tacgaaagta gcttcaacta tgaagattca ttgatttcat ctactgcatg
     2221 ttgtgttgaa ttgataaaat ctggggtaac tacattttta gaatctggtg gtcaatatgt
     2281 agatgctatg gttgaagctg ttgataaaac tggacttaga gcctgtttag ctaaatcagt
     2341 tatggataca ggagatggtc ttcctgaagc atggcaaaaa actactgatg aggaaatcaa
     2401 cactcaatta gacttattcc ataagtttaa taacacatct aatgagagga ttaaaatatg
     2461 gtttgcatta agaactattt ttaatacaac tgaagaattg cttattagga ctaaagagtt
     2521 agctgataaa tacaatacag gtatacatat gcatattgct gaaatagctg atgaaatttc
     2581 atttgtaaag caaaacaacg gagttggaac agttgaatat cttgataaac taggtgtatt
     2641 aggaccaaat cttttagctg ctcacacagt ttggcttact cataaagaaa ttgatttatt
     2701 tagattacat gatgtaaaag tttctcataa tccaggtgca gctatgaaag tagttctagg
     2761 atttgcctca attcctgaaa tgttagaaaa aggaatacct gtttcaattg gtactgatgg
     2821 agctccttca aataatagaa tggaccttat gagggatatg tatcttacat cactaataca
     2881 taaaggtaga actttaaacc caactgtcgt accagctgaa gaagtcttag aaatggctac
     2941 tataaatgga gcaaaatgtg ctttactaga aaaagaaatt ggtagtcttg aagtaggaaa
     3001 aaaagctgat atgattattc taaatccaga tacaattcat tgtttaccaa tgcataatcc
     3061 aattggaaat atagtttatt ctatgactag tgaaaatgta gattctacta tttgtgatgg
     3121 taaatggttg atgaaggaaa gaaaagtatt agttgtagat gaatctgaat tattaaataa
     3181 agttaaaaaa caagcttcta aaataaaaga taaagcagaa gttaaaattc catctaaatt
     3241 taaaatcata aaataacata aatttcaatt gttttgtaaa tatgaatata taaatatatt
     3301 tatacacatc atatatacaa aacaattgtt tttttatttt atattcatta acattgctca
     3361 taaaatacac cataattcca gaaaaaataa ccaatcagtt aggattacat catctaagca
     3421 atatataaac gtcttagttt ataagtttta ttctactctt atctaaaacc atctttaaag
     3481 agttttcaac tgtttcaatt acatctttat aactcaagta cacatttgcg tagatacaat
     3541 cttcatataa attatattca gttactctac aatacagcag ttttcctcca tctaataaat
     3601 tgctcaaaat atagttttct tttcttggga catagcctat tacatatcca gtttcaccaa
     3661 ctattttaat agctttttca tcatatagat tatctttctc tctagttaaa tagtatatct
     3721 tactcttctc caattctttt tcaagatatt ctctatttct aaaccttgaa ccagcaacag
     3781 ctattgaagt caaatagata tccttaccat gttcatctac ttttattttt tcaatattta
     3841 caaattcttt ttttaaatta ttactttcat ataatactct tcttaattca tccttcaata
     3901 taacattttt ttctttttca tacgcttctt taacaatttt taattcttca agagatagtt
     3961 tctctctgta tctttttaag atatttatgg attcttttct tacttcattt atatttccaa
     4021 atattccttt taaagatata ttctttcctt caggatatag tgatttactt cctttaagca
     4081 caataaaaaa taacatatca tcataatatt cttgagtcaa tttatctttt tctatatctt
     4141 tcatttttcc aattaaatca tctattttaa acgtctcttc aaaaaaattt aataatttta
     4201 atctagaact tgtcgttccc tttttagata tatgccaata tacctcaaaa tctctaatat
     4261 ctctattcag atatggcaac aagtcatcaa aagataagtt tacatcataa atttctgaca
     4321 tttttataat atcttctgtt tttccactag catctcttaa tgcttcaaaa tatatatttt
     4381 tccatttttc actgagcaag atatcattta tttctttttc aaattcaatc ttattgtact
     4441 ctatattttc atcttttaag acgtttatag ctattaggaa aattgaatat aaactttcaa
     4501 agttagtccc ataatttact acagtaggta aaaatcgatt cactaattct aatttattac
     4561 ctatatattt aaattctaca cttagcaagt aattgcatat aagacgtgct aaattattta
     4621 tcttttcttt atctaaatct cgtttttcaa gatattcatt caaatctaca attgaaataa
     4681 tatagttcat caataatcgt tcgtactttg tatccttata accatcttca attagatagg
     4741 aatttatttt agaatctaaa ttttccatat taacaatagc aaatactttt attgaacctg
     4801 tagccttttt agataaatta aataaatatg tattgtaaaa ttcaagtttt ttaatagtat
     4861 tactcagata aaatacgtat tctccagatt tagaaaatgt atctacaact tctcttaagt
     4921 tttctacatt tagatatttc tcacttaaaa ctaaccctag ttttacatac tcaacttttt
     4981 tactcttata tattaattct ttagttaatt tatataacca tttctcatca ataatcattt
     5041 cttttaccaa agtatttatc tttttcataa aaatttcgaa ataacaaatt aaagacttag
     5101 aattactatc ctccaataaa taaatttcat tagattcact tttatacata tttaaaattt
     5161 catctactag attatttgca agttctactt gttctcttaa cggtatactt tcatatatac
     5221 ataggtaatt catttcaaat gattctttgt atccatcata accattaaat tcctgcaatc
     5281 gcttaaaaac attctccacc aaattatcct cctttacatg atgtaattta tattgattat
     5341 actacttatt tcgtcaagtt tacatttata cgcaaaaaaa tattactaga aattgaaatt
     5401 ctagtaatat ccttattatt aattatgtaa ataccctatc ttataactgt aaataatatc
     5461 cttattaatt ttaattactt ttaataaaaa tctatcctag atttagactt ctctatgcat
     5521 atattatact aatattagac atcataatca tacacttttt ctttttcata tctcatgttt
     5581 acaaacaatg caagtaaaat tccaacaaat gttattgcca cattaaacaa tacaacatat
     5641 tttggtccat taactccccc tgattttgta ataatacctg ctatatttaa aataacatag
     5701 tttgctatac tagatgcaat cataactata gatgttattt ttcctttatt agttggaaac
     5761 atttcatttg cagttgacgt tgttaactga agaacacctc ctgctgctga ataacctatt
     5821 acaaatcccc ctaacataca aatagttgga gtttgaacaa aatatacaat taatagcata
     5881 agtaaagcta ttataggata tataactaat attcttattg gtttaatata ttttttaata
     5941 agtaaagaag ttattaatac tgcacatatt actcccattg agtaaaagga ttgaattttt
     6001 gtagggtcag ccatcccata caatttaccc aattcctgat tacaatttaa ccataataca
     6061 aatgtagatg tacatgtaaa tccaatacaa acaagagcta tacttgttgg tgtaaatttc
     6121 atcttctctg atttaactgt tttgcctttg ttatcaataa cattattagc tggtggaaat
     6181 ggtaacacag ctatcaaaat ggcatctact acaataagaa tcgctgttac tataaaaata
     6241 actttaaatg atattgaatt tgctgcaaca aatccaatta taaaaggaag caaaaattgt
     6301 gctaaagcta ttgtaaattt agtaaacata tttgctattg ttcctaaact agcaaatatc
     6361 tccatacaag aaggagttac acaggtatct aagaatgaat ttgctgcccc tccaattatt
     6421 gcaaatacat atgctatata aaaatttggt gaaaatacaa ttccaacaaa gaaaattgca
     6481 tgtagaaagt ttcctataag accacttact tttcttccat acttatctga aataggtcct
     6541 gcaaatggat aagatataag tctccctaat cctagtgcag caataacagc taaaacaata
     6601 cttacatcca atgttccatc tgatagtgtt ttagcccccc acattgatga aaattctggt
     6661 ttgtattgtc ctaaaattga aattccaata ccaagtaaaa aatatgaaaa atacaacgct
     6721 atggcagtag gataatattt tttattgttc ataatttttc tcccattctt gttaatttat
     6781 taacattttt gtgtacaata aactcatcct cataatggat ttaaagtata gtcagttaaa
     6841 gcttcaaatt taactggact atgatgcaaa acccactatg acaatttgta atttattatt
     6901 aaaaatattg aaatacgtat gttttttcat tgtttaagat atcactctta tctatttaaa
     6961 gcataattca ttaacttctt ctacaggcat ttctaatcca gtatatagat taaaagcttc
     7021 tgctccttgg tataataaca ttcctaatcc accaatagcc ttacatccat tagcttcagc
     7081 atcttcaatc atttttgttt tcttaggatt ataaacgaca tctgttacta ctaagtcttt
     7141 tcttaaaaca ctagtatctt ttatatttgt ttcattgtca taaggcttca taccaataag
     7201 agttgcattt gtcaatatat cacttgattc tatctcctca taaagtttgt ttgtatcttc
     7261 taaatcatat agattaacaa cacattctgg tacatctttt tttatattct caactgtctg
     7321 ctctgctctt ttataaaaat catccttagc attaaagata gatatttctc ttgctccatc
     7381 taaagcacat tgtacttgta tagcagttgc tgcaccacca gcacccataa ttgtaatctt
     7441 tttaccttta acttctatcc cattttcttt taaattacga acatagccta ctccatctgt
     7501 tatatgacca actaactttc cattgtcatt tacaattgta ttacatgctc caattatacg
     7561 agctgctgga gaaagttcat ccatatattc tgtaacagca gatttacatg gcatagtaac
     7621 attggctcct tttatattaa aagtttttat tgctaaaagt gcctctttta ccttatctac
     7681 tgtgatatca aaggctaaat atctataatc taaatctaat ttcttaaaac tataattata
     7741 catcactggt gatttagagt gaccaactgg tgtacctatt aatgcaaata atcctgttct
     7801 acctgaaatt tccataacta taatttcctc ccaatattat cattttcttt gcaaataacc
     7861 tattcagtta tttacaaaga aaaatttatt tattatttat atatttaatg ctacttcaaa
     7921 agcttctttt gttgcatcta atgccctacg agcttttact gcatcaccaa caatataagt
     7981 ttcttttaca acttctttta taacttcact taatgggtcg taattacgag aacccatagc
     8041 aagtactact gaatcaaaac cttttaaatc atgctctgtg ccatctgtaa gtaaatagtc
     8101 gacaccatca tcataaaatt tagaaacttt tgcaccagta atggtatgta tattataatt
     8161 ttcaaaatca ttcataagga atttacggtg ttctgaaatt acatctgcac caacttcttc
     8221 tctaagctca ataacagtaa cctcatgttc ttgttctcca aggaaggctg ccacttcaca
     8281 tccaaccatt ccacctccaa caacaagtac ttttttccca caagattttt taccatcaag
     8341 aaggtctaca gcatgaatta atcctgaatc attgattcct ggtataggaa gtacaagtgg
     8401 agttgctcca gtagctataa taactacatc aggtgatttc tcctttataa gttctactgt
     8461 agcttctgta ttagtacata tttttacacc atattgatta cattttgaaa tataacttct
     8521 aactaagtta gtaatatctc ctttccctgg aggatatgca gctaaacgca tttgcccacc
     8581 aagaacttca gatttttcaa ctaaagttac attatgtcct cttttagcac atacccatgc
     8641 tgcaagcata cctccaacac ctccaccaac aacaagaact tctttgctaa tttcagctgg
     8701 cttaagctct gcttcatgtc ctaatatagg atttgcaaga caggctatag gttttccttg
     8761 gaacatattt ggtacacatc cttgtaagca tgcaatacat ggtatcattt cgtctagttt
     8821 tccattttta gctttattag gcatttctgg gtcagccaag ctttgtctac cgaatgctac
     8881 taaatcacat cttccttgtc tgaccattaa ctcagcaaac tgtggctctg tataacgtcc
     8941 aactgttatt actggtatac taacagcttt ttttatttca gtaactaatt ctgcactgaa
     9001 tccagcatgt aaagtcgtag gagcccacat atattcatca tgtatatgta tactacgtga
     9061 aacatgaagt ccatctattc cacaaccttc tagatatgct gctattactg aactatcatg
     9121 tatatcaatt cctcctggaa catcatcagt gctgtttata cgacataata ttgctagaga
     9181 atttcctact tttttacgta tattttcaat gataagacga ggtaatctca ttctattttc
     9241 aaaacatcca ccaaactcat ctacacgctt atttgttctt tgtgatataa aactactaac
     9301 cagatatcca tgagcgcagt gaacttcaac agcatctgca ccagcttttt gtgcacgtaa
     9361 tgcagcatct ccataagatt ctataagttc atatatttct tctgttgtta ttgctagtgg
     9421 tgtattacga ccatttgatg caggtattgc agaagcagct tttaatggat gtcctgctac
     9481 tttttcattt ccttcagaac cagcatgttg taattgtata gataccttcg aacctgcatc
     9541 atgacaagca tctattacat ttttaaaact atctattgtg ctatcatcat ataaacaagg
     9601 cttacgagga cctccttttg cttttttgtc tacaacacta gattctatag ttattaatcc
     9661 aaaaccacct aaagcacgct caagataata agccttagaa gtttcactta aagtaccgtc
     9721 tgtgtttgca aaattgtttc ccataggggg aactacaaaa cgatttggaa ctgtcattga
     9781 accaattctt ataggtgtaa acatagaact aatcttcata aggttttcct tcctttcaag
     9841 ttttatattt aaaagtttta tattataata ctctgtatat ttttatatta tataagttta
     9901 ttttggagat acttcagacc atgctttatc caaaactttt ttagttgcat taaatacagt
     9961 ttcagcagca gtggataacc ctttagatat tagagaatca gatattactt ctgcccctat
    10021 gactcttggt gatatattgt gttcttttat tattttcaca aattcctctg tgtttccata
    10081 tccttcacct ggagccaaac ggtcatgtag tgattcatcc ctaagttttt cataaggagt
    10141 ttctaaaaca tcacacactt gaattgatac tatcttatct gctggaacag gtgcaaggtc
    10201 ctctgctgtc tgctttgctc tagcccagtg ccaagtatct aatataagca ttgcattttc
    10261 acgattacat cctttcacaa cacgccatgc agtagccaaa tctggtacac cactatatgg
    10321 cataaactca agacctataa taagttcacc tgctctgtca cataattctc ccaatgcagt
    10381 tataatttgt tcttctggta tcatctctaa taatccacaa tttatatgtt taacattaaa
    10441 taagtgagcc atatgataaa cattttgctc tttttctctt tgagcttcag ttctatcttc
    10501 ttctgttccc cattgagtta tatattctac ttctgtaact ttcatatcat attcattaag
    10561 aattgcaatc atttcttcat ctgtaacacc tgcatttttt gcatcaatat aattttcagc
    10621 acgcaatcca ataccatcaa atccagcttc ttttgcaata cgaactctat cacggaatga
    10681 aacttctgtt cccaatgtat aagaactaat tgtaattgga atattaagat ttttttgatt
    10741 attcatagct taaatttgcc ccctttattt tctttataat taatagtatc tttgatttta
    10801 tatttttatt tatcctacta gtagtattat tttaccttaa aaataaaagt attttttgat
    10861 ttctatttat ctagatttta taaatttatt gtatcacgag actttactaa taacaaattg
    10921 ataatttcct attaattcat agattttgtt tatagatttt atttataaga taattcttgt
    10981 atttttctat taggttgtat aaatatattt atcttataat tcagacaaaa aacgataaat
    11041 ttttttaaaa gcttttattc ttactctata atcatatata ataaaaatgt aaaaatatct
    11101 taaaggttaa cttctacaaa tatataaata aaaataccta tgaaaggatg aacaaaaatt
    11161 gaatttatat cacttacgct attttgttac attggcacat ttagaacact atacaaaagc
    11221 ggcagaaaat ttatctataa cacaaccaag cttaagtcat gctatttctt tactagaaaa
    11281 tgaacttggg gtggctctat ttgaaaaaga aggtagaaat atagtcctta ctaagtatgg
    11341 aaaaatattt ttaaaagatg tggaaaaatc attagaaatt ttagattcaa gtgtaaaatc
    11401 attaaaaata actggtactg gtgaaggaca aatagactta gcttttctta gaacacttgg
    11461 tactgatttt atcccagaca tagtgcataa atttttaaaa tctaatcctg ctaaatcaat
    11521 agattttaaa tttcatactg gtgtaactac tgacattatt caaggtctta aagaaagaaa
    11581 atatgatatt gcattttgtt cgaaacttga aaaagaaaag ggaattgaat ttatacctgt
    11641 tgccaaacaa gatttagtac taattgtacc atatagtcat ccactagctg caaaggatac
    11701 tatagattta aaagaaacca taccttatcc tcaaatagta tttaatcaga gaagtggtct
    11761 tagatatata attgatgata tgtttaaaaa aataaatcaa cagcctaata tagtctatga
    11821 agtagaggaa gaccaagtaa ttgctggatt agttgcaaaa aatttcggaa ttgctgtagt
    11881 cccaaatatg aatatgctta gctttacaaa agtaaaagta attcagatta ttcacccaag
    11941 ttgggaaaga aacttttact tagcatttat aaaagataga tatttgcctc ctgcaattaa
    12001 aaactttaaa aactttgtca ttaaaaacgc tcaattataa tttattaata aaggggttat
    12061 ttttaaatat cctcttttat tctttttata aaataaaaaa atctatactt ttaaaagtat
    12121 agattcctaa ctgacagtcg gcatccgagt ctcagtaatt atcgttatac aacagcaact
    12181 ggattcagtt cctgaataac taggttattt acattatatc agattttttt aaatttgtca
    12241 attgtatcta acgcattttt tcaagccatg gtccaacagg ctctacaata ggaacttctt
    12301 taaatcccca atttcttata tcattcacac tcgttggagt atatattttg tttctgacag
    12361 atttgaatat tttgtttttt tctccatatt gcatacactt tggattagct tctattaacc
    12421 ctcttcttgc tctgctaaga ggattacata aaatatctgc tatttctaaa ccttctctat
    12481 agtcagcatc ttcttttttg gcaattataa aacccttaac cttatctctt aattcttcat
    12541 ctatatctaa gtatattgtt ccattattaa atacatcaaa aaacgccttt tgtactttta
    12601 agtttgagac atcatccctt gactctataa ctattcgtgc agattctaca gtttcattat
    12661 ttacaaatga ataaaaattc tgaagaacat gtataaaagc cacaacatac ggttcctttg
    12721 aaggtgcata atatttcttt aatctagctt tatctactgt tactgatatt atagtacact
    12781 ctaatctaga tagaaataaa ggcaactcat catagaataa cttaatctgt gaatcatcaa
    12841 acttctcaaa tgctcttgat tttcttgata tatctgtcaa atgtattcct atgttactgg
    12901 ttccaaaaca atactcttta aatgcatcca tttctttcct caaagaacat acataatcgc
    12961 cttgacttac tgcatattta tattcaaaaa ttactcctgt caatgtaaat gggtcttcat
    13021 gatttatatc agctcttcca ctttcatcaa taaacattat atattttttc tttataatct
    13081 tactcaccct cttaacatat gatgatagca tctatattca ttttatatga gttttaaaaa
    13141 tattccaaga tttttatata acttaaactt aattttatac taaaaaattg agatgtaaaa
    13201 caatcaagct tttattgatt gaatttcaca tctcaattat aattattttt caatatatat
    13261 ttttactatt ttgcaagaat atccattaat ttatccatta ctatataaga tactccgcct
    13321 cctatttgat aaggagtttc aactttttta tttgcaaata ctctttgttg ctctaatgat
    13381 aatcctgaat tatccattaa aactattggt gaatgtaatt ttacagctaa tggtccagct
    13441 gataaagcat ctactaaagc atctgattta ctaactaata cagcctgtaa atcagtatct
    13501 ccatagaatt tttctattac tttggcatta gtatctactc tactatctcc atatattcta
    13561 tttcctgaaa tatcttcagt agttatatca ttcatttttg ctattactga atcgttaatt
    13621 gcacttggtc cacctataaa gtatgcattt tctaattttc tttctgttag ccatttataa
    13681 gattcttctg taattccatc tttattgact aatattatag gttgtttttc ttccccagct
    13741 ttagaagcta cagataaagc atctgcttcc ccatttccag aaaccatata tgcagttttt
    13801 atttcttctc gatttccaag ttcttcagct attcttgtag cagtttgata tctatcttga
    13861 cctgctattc tctttgtctc tgtgttgaaa gtagttttta tttcttgctc taatttagaa
    13921 cttattgcat tttctccacc tatcaagatt actgttttag gtgctaataa cttcatttct
    13981 tcaacagttt cagttggtaa attagctgta ttagtaagta gtattgttga gtcatagcta
    14041 cttgctaaag gagttgctgt tataccatca gctattgcat taccatttac taatattaca
    14101 gtatctgaac tatcagcatt ccagtttcca actgaactta tctttttagc tgtatcaaat
    14161 ctatttgcac ctataattgt atcccatgta ggatttatag ttccatcttc attaaaagta
    14221 taatctttcc catctatttt ttgagaacca gttagcatct ttccatcttt gtctaaatag
    14281 taagacttac catctatttg ttgccatcct gtctgcatta ttcctgtttt gtcaaagtaa
    14341 tacttattac catctatttc ttgccatcct gttactgata aaccatcaga tcctagatag
    14401 tatttctttc cattcttttc ttgccatcct gtttgcatta ctccatctga gttaaagtaa
    14461 taagtttttc cgtctattac tttccatcca gatactcttg ctccatcagt tcctaaataa
    14521 tattttttgt tattaattgc tttccatcct gtttgcatta ctcctgaagc atcaaagtaa
    14581 tacatttttc catttacttt tagccaacta ttcttttggt atgttccatc agattttaca
    14641 taatacttct taccattttc tgttttccat cctgtttgat ttagatttat cttatatttt
    14701 gccgctatct ttacatagct gtcattacta taatctataa ttacatctga acctacagga
    14761 acttggtcaa ataaccatct aacatcttta ttgtgcattc ttatacatcc accagaaata
    14821 tgctttccta tagaactctc attattattt ccatgtattc catatgtatc tccataagtt
    14881 ccttttaatg ctaatcccat ccatctatct cctaatggat ttcttggact tcctccaggt
    14941 atattacctt tataatatgg tctatttttt atcttgttaa ctacttttgt tttccctgtt
    15001 ggtgtttctg accccttttt acctgttgca actctaaatt cttttacaag tttattattt
    15061 acaaaatatc ctagagtatt ctttcttgaa tttactataa tcacatgttt actggctgcg
    15121 cttgattctg gtacgccaca tgcaaatact aaaactaaag tcagtgctag tactaaatac
    15181 ttttttagac tttttttcac tataacttcc ccctctttta gaaacattac tacatgtgtc
    15241 taattataac ataaaattac caatacctta tatttacaaa tttgttacat ttatacatat
    15301 aagtatgtat tctactcttc atacccatta ggattcatac tttgccatct ccatgaatct
    15361 tcacacatct cttcaagttc atacttagct ttccatccaa gctctttttc tgcctttgaa
    15421 gaatctgcat aacacatggc tacatctcca gcccttcttc ctacaatctt atatggtatt
    15481 tcttttccac ttgctttaga aaatgctttt actaaatcaa gaacactata accctttcca
    15541 gttcctaaat tataaacaac aagtcctgga ttttcttcta atttttgtaa tgcttttaca
    15601 tgaccagcag ctaaatctaa tacatgtata tagtctctaa ctccagttcc atcatgtgtt
    15661 ggataatcat taccataaac actcaattcc ttcaatttac ctactgctat ttttgttata
    15721 taaggcatta gattgctcgg aactccattt ggttcttcac ctattctacc acttttatga
    15781 gcaccaactg gattaaagta tcttaaaaga gctatatcca atgacttatc tgcttttgat
    15841 atatctacta acatctgctc tatcataagt ttagttcttc catatggatt ggttacagaa
    15901 agtgggaaat cctctaatat aggacaagta tgtgggtctc catatacagt agctgaagaa
    15961 ctaaatacaa actttttaac accatattct ctcattaatt caaataaatt taaggtactt
    16021 attagattat tgctgtaata ttcaattggt ttttctactg attcaccaac tgcttttaaa
    16081 gctgcaaagt gtataattga ttcaatatta ttttctttaa atacaacatg catttcatct
    16141 ttatttctga tatcaatatt ataaaatttt actggttttt tagataattc cttaattcta
    16201 tcaaccacta ttgagttgct attacttaag ttatcaacta taacaacttc ataacctgat
    16261 tctaaaagtt ctattgctgt atggcttcct atatatcctg ctcctccagc tactaaaact
    16321 gccattgtaa ttacctcttt tctaatattt tatttttcac ttactttttt tctcatatag
    16381 tccattaaat catcttttaa attttctctc tttagagcaa agtctattgt tgcctctaaa
    16441 aaaccaatct tatctcccac atcatatctt ctaccttcaa agttataagc ataaatagcc
    16501 tcttgttttc caagagtttg aagtgcatct gtcagttgta tctctccacc tttaccaggt
    16561 gtctgtttct ctaatatact aaatattgca ggagttatta tatatcttcc aagtatagct
    16621 atatcagatg gagcttcttc aattgcaggt ttttcaacca tatctttaac tttatatact
    16681 ctatcttcga tatgttttac atccaatata ccatatttat tagtatcttc tttagctatc
    16741 ttttgaacac ctaatacagt tgttttatac tcattataag tatctattaa ttgttttaag
    16801 cacggaacat ctgaatctac tatatcatca cctaataata cggcaaaagg ttcatcacct
    16861 ataaaacttt ttgcacagta aattgcatgt ccaagacctt ttggctcttt ttgtcttata
    16921 taatgaatat ttaccatatt tgatatgtct cttaccattt ctaacatctc tgtcttacct
    16981 ttttgctcta gttctaactc taattcaact gatttatcaa aatgatcttc tatagatttt
    17041 ttatttctac ctgttattat aagaatttct tcaattcccg attctatagc ttcttctatt
    17101 atatactgta aagttggttt atctactata ggaagcattt ctttgggctg tgattttgtt
    17161 gctggtaaaa atctggttcc tagccctgct gctggtatta ttgctttttt aactgtcatc
    17221 tgcatttttt cacccttctt cgtaaatttt ctattgtacc aaattaatat accatattta
    17281 tactttttct tcaaactcta tattatccca atatttaatg ctataagata atataaggta
    17341 gatgaaattt catctacctt tagttttata agttaatatt gtattgttta tattttatcc
    17401 aaaacttata caggtactaa atttcatcta agatgccttt tatatcacta tctatatctt
    17461 catctagata agaatctaaa tcttctaagt caacttctag ttcttgatat gtcatatcaa
    17521 cactgacatc ctcatctata tccatttctg atattaattc tcctaaaata ctatttccat
    17581 cagaatctaa accactatct atatcttcat ctatatagtt ttcatttaaa atttcatcta
    17641 aatcaatctc gtaataaact tcattatttt tttcatcccc tgagtccaat cctaagaact
    17701 catagttaaa agtccaaaca ttgtatttac ttgacatact ttattcctcc tgtgaaaaac
    17761 atattttcat ctagtttatt ggttccataa tcaacagagt tatatatttt ttcaattatg
    17821 tcttttcttc tttcttttgt actattattt aatgtcaatt gtttaaatac atatgtattt
    17881 gttattctct cgaataatat ttcttttata ctatcatcat caatatttct atagtttttc
    17941 cacaataact caaataactt aaacatttcc ctactagttt catctacata ataaggatac
    18001 atattttcta ctaaatggtt taatcttgat atggcttgtt ttaaaaatac atcaagactt
    18061 gcactttcac tttcttttaa cattctataa ttgtatttct tcttatcttc tcctgaaatt
    18121 acaaagatga tattgtctaa acttttttca acttcattta aataactgct tatattatat
    18181 gataagtcag acaatagatt tttaatctgc ttaaacactt gtacatcaag tttagtatta
    18241 tacttttcaa acaaatctat ttcttgattt tctaatattc ttggcatata tgctgacaat
    18301 aaatctaaat tgtttctttg ttcttgtatt tgcttataaa gtgaatttat aacttctatt
    18361 cttatttcat catttaactc tttttctaaa atcttatcaa attcagattt catatagtaa
    18421 atatttccaa aaaaattaaa tattatcata tcattcatag ctttagacca attcttatat
    18481 atcacttcaa tagaattttc tttaagtaga ggttgctttg catatataaa tacatcttgc
    18541 ataagttttt gaaccatatt tttacttaag tttaaaagta ctttttgatt attaaatttt
    18601 atttccgttt ctacaaaatt agaattttcg ttttcaagtt ctttattaat tacaatgtct
    18661 tttatatcac tattttttat agatatgatt tctttttgaa aagactttag aatagtcaag
    18721 ctatcgaaat cttcttgaat aaaaaatgga gcatgtttta atgtatatcc cactatttca
    18781 ttttctatat cttttaagtc atctatttgc ataatttctc tatttttatc atttatatgt
    18841 aggttaaata catttttatt atcatataat attatatctt ctcctaaatt taacttagaa
    18901 caattatctt tcgctgactt tacaatagtc ttcttagatt tcttatttat aaaaattaat
    18961 tggtcttgat attttactaa aagcatgttt gtgtttttat aatcaaatcc atctatattt
    19021 cctgatatct tgcagaatac tatatcacaa tctttaggaa gatttcctat tttttggtta
    19081 tctttaactc tctcatttaa atcagatgga actacactat ctatattgtt attaaatata
    19141 gatacagacc ttactataat cccctctctt tccatatgaa agtatccctt caatgacact
    19201 cttttatctt ctaaaatgta tctttctata tcatcaaaat ttatttcaaa tctatttttc
    19261 ccactgataa atataaactt ctcattatct ttattaattc cacccatata aaacttattg
    19321 tcatctaaac ttaatatagt catattatcc tttagataga atataaattc tctttgactt
    19381 ttgcagatta aatcatattc atccaaagtt attttcatag tcaaatttgc tgtaacattt
    19441 ttacttgaat tttcactttt tatgtaaaaa ggcaagttat ttccatagac aattatttga
    19501 gaatcttcta aaactgcctc taatttttct ttattgtctg aaaaactata tatatatttt
    19561 acacctgtat cctcattaac ttcatctgat ttgctgatta ttatattccc tagatttgtt
    19621 atatgaagct ttttatattc ttttgagtat ctacttatgc cactaagctc aaacccatca
    19681 gaaagctcat atatgatttt ttctcctaat ttcaacatac cttcaccacc taaacagaat
    19741 tataagtatt ttataaaggt gaataaaatg tctcaaaaca aataatattt ttttaatact
    19801 ttttattctg agacatagtt tctccttttt tataatgctc taagcacctt caactatttc
    19861 ttcataaagt ttatctaaat gtttaacata ttcacgatat ccagatttgt tctttaactc
    19921 acttcttaaa tcttcatatt tttgtttaag ttcttcagca ctcaaagaag gagctgattc
    19981 ttttaattct tctaacttat aatcatagta cttatcatac atagctatat atgtatcaat
    20041 agatttctta gctagttcat tacatttttc ttgtgcttct ccatattgag aataatcacc
    20101 tatttttatt ttatagtatc cattttcttg aattattgtt gaagaaatac ctttagcctc
    20161 atattctttt gctgtttctt ttgctgtttt ttcaacttta agtgaacctg cataaacagt
    20221 gtaatataca cctttaccat ctttatatgc ttccatttct tttgctttct tttcagcttg
    20281 tgctttttct tgagcctctt tttcagcttt tgccttttct tctgcttctt ttttctcttt
    20341 ttcagcttgt gcttttttct cttcctcagt taatttattg ttagcttgtt gttgagttga
    20401 tgactttgga atattctctt tatcattttt cttattgttg cctactgtta taaaatatcc
    20461 tacaccaaca ataaccacta taactactgc tataatagtt tcttttaatc tactcttctt
    20521 agaaacaacc tcttcatcat ctaaagaatc ttctttatct gatacataat ttttcatttc
    20581 tttcttgttt tctctatatt tgtccttaga agaattttta gattttttat tactattttc
    20641 tttcttttta ataggtatta ctactgattt ttcattttct aatttttctt cttttatttc
    20701 ttttgctttt ttatcttcta agtttttact ttctttttca agcttcgatt cattatttga
    20761 agatactttt tcatctaagt tattatttat tggtttttct atttttacac tacgttcttc
    20821 ttctatatgt tgacttggtt cttcttgttt tatttctttt aattcatttt gctctatttc
    20881 ttctgatttt gcttgatgat tttcaactgg tttatctaaa attatatcag gagaaatatc
    20941 atttaaattc tcttcttctt gttcaacttc ttcattgtct tttttaataa atccaaataa
    21001 tttagccttc tttttactct ttttattttc tggttcaatt atatcttctt tattgctgtc
    21061 gtactcttta tctgtagtat ctttatcttc tacaacgtca cctatatctt gattattact
    21121 atctacttct tctttagccc cttcttcagc atcatcttct attgaaatac tacttctaaa
    21181 gcaaatatct ttatctgcat tttttatcat gtaaatgtat tcttcaatat cgatatcttt
    21241 atttcttcta gttctttttg atgcaatttc atgattttct aattcactat agttatcttt
    21301 tattagattc tgtgctttat tccatgtatc ttcactaatt tctgttttat ttttaattac
    21361 atagtccaca aattccttaa aattttcagg accaaattct ccaattatag tcagtctcat
    21421 tgcaccttct ataaggtctc tatttcttct ctttaaaaat tctaatacaa ccgttgcaca
    21481 agcaaaatct ttaataattt ctttgccaaa accatatcct tcaacagaat gaagttcttc
    21541 taatccacat aacttcttat attcattgtg tattgtttga gatatacttt cattatttaa
    21601 gctatttaac ttatctataa acttcgattc tatatcattt gattttgatt catgttttac
    21661 ttttgaattt ttaatttcat ttagtagcat gtcattttct gaattatctt tacctaattg
    21721 caaatcctca atttcagttg cttttatttc tactttgtct tgttcgttat tctcttccac
    21781 attattaggt accacatctt tattgctagc taatattttg tttacacaga atataatttc
    21841 tctttttaat tctcttttcg catctgcttg atttttaaga aataaatctc cacattcttt
    21901 cttttctaaa cttatttttc taagagatat attaaggagg tctttatata ggtttaaaac
    21961 cttataatcc ttttctaaac gtcttcttct ctctgatagt tccattttat cataagctaa
    22021 aaaactattt aaatcattgg cttttagaga gtctaatccc tctttcatag ccttatttac
    22081 atactttcta cattttgctg tagttaattc taaatctttt tctaattcat ctatttcagg
    22141 tatgtactta tctactatcc atttgccaat aagtggttct gtatttcttg ctaatgtctt
    22201 ataaaacaat atattaaatt ttaaaagctc tgcatatttt gcttcattaa attctacttt
    22261 ttcaaatttc ttttccaaaa caccatctag cttttcattt attttagact cttgttttaa
    22321 aaaacttgct gtgacattta aaacagtgtt tcttattata ttattttcac tcaaaacctt
    22381 cactccttaa atcaattatt ttattgtaat atttacattg tgcaaagttg ctaatttgat
    22441 tatgatatct aaataattat ttactaaatt tgctacatct tatatcatat catattattt
    22501 acatcatttt actttttctt tttatatcta tcttatatta taccattttt aatatctgta
    22561 ctaacttata ttacaatatt cctcaaaatt ttaaaagtag attagacaaa tttacaaata
    22621 aaaaaacatc agtttaaact gatgtttttg actatcgaaa taattaagca agtctataag
    22681 cggagttctg tattagataa tcatctttct aggcctattg tcaccaatag gctcaagcga
    22741 cctacctacc ggagagtacg agcagcactc tttattccta tcttggtctt gctccaggtg
    22801 gggtttacat agccaactag tcgcctagct gctggtgagc tcttacctcg cctttccatc
    22861 cttaccacaa tagtggcggt ttatttctgt tgcactatcc ttaggatcgc tcccactagg
    22921 cgttacctag caccctgctc tatggagctc cgactttcct catgtaagat tacatgcgat
    22981 tatctggctt acttaatatt tattttactt agttatgtta acacaaattc ttttacatgt
    23041 caaagtaata ttatactaaa aatttaaatt tattcaaaaa tagtgtaata tagatatata
    23101 aactccattt aactcgaggt gatatattga aaaaattata tagaatagta attaacatta
    23161 tactagtatt agtaatactt tacagtggat ttaatatata ctcaaaactt accaaataca
    23221 atcatgatac taaaacatcc tcagaacttc aaaaaaaaga atataaaaaa gaggatttat
    23281 caaagattaa cagtgatttt aaattttggc tttctgttga gaatacaaat ataaattatc
    23341 cagttgtaca atctaaagat aattcttact atcttgacaa agatttttat aaaaaagatt
    23401 ctatttctgg tacactgttt atggattata gaaataaatc tattgatgac aaaaatatca
    23461 taatatatgg acataatatg aaaaataaaa ctatgttcaa caacctaaat aagtttaaag
    23521 atgctgactt ctttaaaaac aataataaaa taaaaataac cttgaatgga aaagaatttt
    23581 tatatgatgt tttttctgct tatattgtag aatctgatta tgattatcta aaaacaaatt
    23641 ttaacaatga atctgattat caaaattaca taaatgatat tacttctaaa tcattgtata
    23701 aatctcctat taaggtaaat agcaatgata aaatagttac actttctaca tgtacctatg
    23761 aatttgatga tgccagaatg gtgattcatg gtagattgat ttaaaactca cagacacagt
    23821 ttaaaacaat aattttaaac tgtgtcatct tttataccta taatttataa attaggccac
    23881 taaaagtatg taatacaaaa taacaaatac atcaaacaat aaaaaacaat aaaaaaagac
    23941 tatgctaaaa acatagtctt tactggagct ggtgatagga atcgaaccta caacctgctg
    24001 attacaagtc agctgctcta ccgttgagcc acaccagcac acctattcta ttgtaaatta
    24061 gtaaaactaa tttttaaaag ttaacactct aaaaactaca catatatctt aatgaatgaa
    24121 catcaaat
//
LOCUS       DAELSY010000054        23893 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.054, whole genome shotgun sequence.
ACCESSION   DAELSY010000054 DAELSY010000000
VERSION     DAELSY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23893
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.054"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            293..1723
                     /locus_tag="KPG09_001844"
     CDS             293..1723
                     /locus_tag="KPG09_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0966455.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1826..2929
                     /locus_tag="KPG09_001845"
     CDS             1826..2929
                     /locus_tag="KPG09_001845"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG0966456.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMTYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            2944..3180
                     /locus_tag="KPG09_001846"
     CDS             2944..3180
                     /locus_tag="KPG09_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0966457.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEKACQEA"
     gene            3197..4246
                     /gene="vorB"
                     /locus_tag="KPG09_001847"
     CDS             3197..4246
                     /gene="vorB"
                     /locus_tag="KPG09_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG0966458.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4248..4988
                     /locus_tag="KPG09_001848"
     CDS             4248..4988
                     /locus_tag="KPG09_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG0966459.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            4991..5530
                     /locus_tag="KPG09_001849"
     CDS             4991..5530
                     /locus_tag="KPG09_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG0966460.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLLEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5654..7753
                     /locus_tag="KPG09_001850"
     CDS             5654..7753
                     /locus_tag="KPG09_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBG0966461.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKMIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWALDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNTKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(8020..8535)
                     /locus_tag="KPG09_001851"
     CDS             complement(8020..8535)
                     /locus_tag="KPG09_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBG0966462.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8627..9838)
                     /locus_tag="KPG09_001852"
     CDS             complement(8627..9838)
                     /locus_tag="KPG09_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG0966463.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDFPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
     gene            10514..11956
                     /locus_tag="KPG09_001853"
     CDS             10514..11956
                     /locus_tag="KPG09_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG0966464.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            11998..12351
                     /locus_tag="KPG09_001854"
     CDS             11998..12351
                     /locus_tag="KPG09_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966465.1"
                     /translation="MKKSNIFSIIFPTIIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            12542..13363
                     /locus_tag="KPG09_001855"
     CDS             12542..13363
                     /locus_tag="KPG09_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG0966466.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIKKVKSNNIA"
     gene            13621..15504
                     /locus_tag="KPG09_001856"
     CDS             13621..15504
                     /locus_tag="KPG09_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBG0966467.1"
                     /translation="MFNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            15728..16408
                     /gene="pgmB"
                     /locus_tag="KPG09_001857"
     CDS             15728..16408
                     /gene="pgmB"
                     /locus_tag="KPG09_001857"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG0966468.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            16405..18690
                     /locus_tag="KPG09_001858"
     CDS             16405..18690
                     /locus_tag="KPG09_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG0966469.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNKVASIEKYVSITSTRDYKESELLR
                     KCEDILNIELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNCIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKIIVYGKKYILEKYIKIDK"
     gene            18916..19809
                     /locus_tag="KPG09_001859"
     CDS             18916..19809
                     /locus_tag="KPG09_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG0966470.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIAEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEV"
     gene            19857..22157
                     /locus_tag="KPG09_001860"
     CDS             19857..22157
                     /locus_tag="KPG09_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966471.1"
                     /translation="MKKIFSIFISCMVVVTTITGCSSGEVIKNTSPINSVKNLDFSFE
                     VKPEIFEVSVESGGSKEKISEPSKERKVSNFKKNKTEASWTYPDENIDINIKKEDSYL
                     DVNIKSNLEKETSFNWPKVEGESYILPLYEGKFIPSNDKYWKEYLNESTYNVIESFSM
                     QFFAVNKAKYSVIYIIENEFNNVIEFSSKDKINFTFKHEYPSINKNKEYGFKIYLTNK
                     NITDVAKTYKNYIVDKGEFKTLKEKSKENKNIEKLYGAPHIYFGDKSIITINDIEWNK
                     LKENLSDDLTNWMKELLSMKSGNSEFIKVINQIKTQDYIDKYQKNQIVQAFNEVMQMN
                     TFYNEKVFESKDREIKKVLDKGISKLSRVELIEFNKKLLKSKLEESVNDVATWAKYNT
                     TDVINDINKSGIKNAWIGFDDMEEGYISLEMVKRANELGYLVGPYDSYHSIHKPGEEK
                     WSTASFKDKSLYENATIENKKGEKETGFQGEGRKLNPTLSLPSVKERVQSILDEGYEF
                     NSWFIDCDATGEIYDDYSQNHITTQTQDLNARLERMSYIRDHANMVIGSEGGNDFSSS
                     TIAFAHGIETPAFAWVDKDMSKNKGSEYYVGKYYSPTGGVPEIFSKQIPVKERYKKIF
                     IDPIYSLPLFRLVYNDSVISSHQWLWGTLKIKDEVGSRMMKEVLYNTPPMYYIDRTEW
                     EKHKDEIISNNKVWSAFNKKAIKKEMTDFKFISEDRLVQMTEYGEDLKVLVNFSQKDI
                     KYNDEVIKANSLVIYDADSKVIYKPE"
     gene            22391..22882
                     /locus_tag="KPG09_001861"
     CDS             22391..22882
                     /locus_tag="KPG09_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBG0966472.1"
                     /translation="MYENNKVIYNFERCEKLENALYLSQFNTYKNFKLTSKKTVINSL
                     DTSTYNMNIHSISLVDTMECISSEDEQMTGKKLILVGDIDTDVTLDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            22910..23857
                     /locus_tag="KPG09_001862"
     CDS             22910..23857
                     /locus_tag="KPG09_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966473.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNSIVFGCIEVEIDYSCKNR
                     SNFKTVNKFNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
BASE COUNT         9529 a         2509 c         4232 g         7623 t
ORIGIN      
        1 agaaatatga taaaataatt taaaatacgc atttcaatga catttcaaga caagaaaata
       61 ataaaaaaca aatctaaatt aagcctaaat agctttttag ttaattttag tagtaaaaaa
      121 taaaacttaa aaagtgtttg acaattatga attttaagaa tataattaaa ttaaaattat
      181 acacatataa aaatatactc ctgaatcaag aatgagatat ttaagattca aaatatatat
      241 tattatacgt gaataatgaa aagtaagaaa attttataag ggaggggtag atgtgagtag
      301 tcaagaaagc aaaccaaaag gaaatcttat atcaaatttt agagatgata tgagagaccc
      361 taagaaaaga gcaatataca catattcaag ttctataaac ttagcgtaca attttatagg
      421 ttactttgta tcaactgtta caaatatttt tttaactgat gtggcaaaac ttggtgtaat
      481 tatggcaggt ggtatgcaaa ttatacaatc tttcataaaa attgctgtag ctcctataac
      541 aggtacagta tttgacaaac aaccattcaa aaaaggaaag tattatccat ggcttagata
      601 tgcaccagca gcgttagcat taacatatgg aatatttttc atagttccat taatgaaaat
      661 aagtccagca ttacttttac ctttgatgac agctttatta ttaatagcat cttgtgctca
      721 gcaagtagtt ttcactataa tttatgctat ttaccctgta gttgctaaga cacctagaga
      781 ccgagttata ggctcaacta ctactaatat atttaaagaa gttggtaaat tcttagtagg
      841 tttttcatat ccgttattat tagttttatt tgcagatgta ttaggtggag aaggaatggg
      901 ttatttaggt acctacttat tctttgcagc aggatgcctt ttagtatttt ggttttcttc
      961 aacagagata ataaaatctg gagcagaaga agaagttctt caaaaagaag agaagccaaa
     1021 gattaaagca agtgaaatgg caaaagctct attcactaat gtaccactta tgatggcatt
     1081 ttgtttagaa tttcttatat gtataagatc tataggatta ggacctttgg ctccatatta
     1141 ttttaaatat gtaatagaag atgaacgtgg attagcagta tttttatcaa tcatgccttt
     1201 ggtaagtgta gcattcatgt ttttcgcacc agtgtttata aaaatctgta gagagcaaaa
     1261 actagcttca ataatttctt tttctatatg tgcactatgc cacttattgg ttgcatggac
     1321 tccttggggg aaaactactt taggagtaac tatgttgctg gcaattggtg gaggtttttc
     1381 aaatgtagta tcaataataa atcttaattt ctttgccggt tcttgtgatt atgggcattg
     1441 gaaatcagga aaagacttac ctggcttaag tatgtcacta tatccagttg caatacaagt
     1501 aggagtatta cttgcaacta ctataagaac agttttaatg aactcaatgg gatatcaagc
     1561 tgatatggta gtcacagaag cagttaaatc aggttttata aatatgattt cttattctat
     1621 ggcaatacct ttaataatag ctgttgttat agcaatacta tatccagtaa gtgacaagaa
     1681 attaaaccaa ataagagaag agttaaatca aagaaatgct tagttaaaag aaagttaagt
     1741 agatggttaa tttaagctat taaagtaagt agacgcaata gcgtttatat aaatcaaaat
     1801 acatatacag agggggcttt taaaaatgat agaaaaatta ttgagaaata ccgtaaaaga
     1861 attacatcaa tatgttccag gtgaaccaat agaaaaagtt aaggaaaaat atggtgtaaa
     1921 agaaataata aaattagctt caaatgaaaa tcctctagga ccatcaccaa aagcaataga
     1981 agctatgatt gaaatgctaa aacaaggtca actttatcca gagccagaag ctaacgaact
     2041 tagaagaaaa ttagctgaaa agctagattt aaaaccagaa aattttattg ttgctaatgg
     2101 tgcggataac gttatcactc taataggtga ggcttttata aatagaggag atgaagttat
     2161 atattgtaac ccaacatttc catcatatag aactgcaact ataaaaaatg aagggatacc
     2221 agtagaagtg cctttaacag aagattataa gtatgattta caaggtatat tagataagat
     2281 tacagataaa acaaaattaa tatgtgtatg taaccctaat aatccaacag gaacaattgt
     2341 ggatgataaa gagttagaag aatttttaaa gaaagtccct gaaaatatta taactatatt
     2401 ggatgaagca tatatagaat ttttaacagt tcctaattat gtggatggac ttaagtatgt
     2461 aagggaaaat tataacgtta tagtaacaag aactttctct aaaatatatg gtcttgcagg
     2521 gcttagagta ggatatggta ttgctaaaga tgagataata agaactttat ttacagtaaa
     2581 agagcctttc tctgcaaatc gtgttgctat aagtggagca acagcagcac ttgatgatga
     2641 agagtttata aaagaaacat atgaattaaa tagagttgga atgacttact ttaaagaaga
     2701 gtttacaaaa atggggtttg atgttgtaga ttcacaatca aatttccttt atgtagatat
     2761 gaaaacagat atacctaagt tatttgaaga cttgaaaaaa agagggtttg taataagacc
     2821 aagtgctaca catgcacgtg taagtattgg aactatggaa gaaaataaga aatttgtagc
     2881 agttttaaag gaaatattaa atattaaagg agagccaagt attggataat agatagaaat
     2941 attatgaaac ggggtgtaga tatgaactta aaaataaacg cagagagatg taaaagttgt
     3001 gaatactgcg ttatatcttg taaaaaagga gcattaaaaa tatcaagtaa gataaataaa
     3061 gaaggttatg ctcatgtaga agttgatgag agtaaatgca tactttgtgg aatttgctat
     3121 caggtgtgtc cagataatgt ttttgaaata attaaagaaa aagcatgtca agaggcttag
     3181 aaggagttgg gataatatga aaaagatgtg gaaggggaac cacgccattg cagaagcagc
     3241 cctaagagga ggttgtgaat tttatgcagg atatccaatt acaccacaaa cagaggtaat
     3301 ggaattttta tcacatagaa tgtctgaact tggaagaact tttatacagt ccgaaaatga
     3361 gatggctgct atatatatgg tatatggagc atatgcaagt ggaatgaggt ctatgacgag
     3421 ttcttcagga ccaggaatat ctttaaaaca agaagggatt tcttacttat gtgcaaatca
     3481 ttacccttgt gtaatcgtaa atgtacagag atgggggcct ggtcttggct cattggactc
     3541 agcacagaca gactatctaa gagatactag agggggagga aatggagatt atcatttgat
     3601 agtttatgca ccaaacagta ttcaagagac tgtagatttg atgtataact catttgatgt
     3661 agcggaaaaa tacagagtgc ctgtcgaaat tttgacagaa gcagctttag gtcaaatgat
     3721 ggaaccagta gaatttccag aatttaagaa gagagaagga gatttaggtt ggacttatga
     3781 tggaagtaat agagaccatg caaaggttgc agataaccaa aagccaactt tttgtatgga
     3841 gaaacgtatg agaataactg aaaatgaaca acaatgggaa gattatcaag tagaagatgc
     3901 agaatatgtg tttgtagcgt tcggtatacc atcaagaaca actatgaatg ctgttagaag
     3961 acttagagaa caaggtgaaa aagttggaat aataagacca ataacagtat ggcctttccc
     4021 atataaagca tttgaaaagg taagtaaaga tgtaaaagga tttatctcag tagaatcaac
     4081 agacacaggt caattagtag aagatgttgc tttagcctca aagaaagtat gtaaagaaaa
     4141 tgtacctgtg tatggtttat ttagtggaaa tcatatacca aaaacattgc aagtaatgga
     4201 cacttatagc aaaataaaat ctggcgaaat caaggaggtg ttctaatatg gctacagaaa
     4261 aattaacacc tagcgttgtt tcaataccaa acaagttttg cccaggttgt ggtcatggaa
     4321 tagtaaatcg tataatagca gaggtaatag aagaacatgg atatgaaaaa aatcatgtat
     4381 taacattagg agttggttgt gtctgcaata tgaattttag ctggaatggt gataagatgc
     4441 aaactgcaca tggtagagct tcatctacag ctataggagt taaagtagca cttccagata
     4501 cattagtaat gacttaccaa ggtgatggag atgcttatgt tataggtctt tctgaaactt
     4561 taaatacagc atatagaaat cataatgtga ctgtatttgt aataaacaat aataattttg
     4621 caatgactgg tggacaaatg tcatggacca ctatgccggg tcaagtcaca acaacaagtg
     4681 taaatggaag agatataaag actactggta gtccactgaa agttcctgag atggtggcaa
     4741 acttctttga tgtagcatat gtagcaagag gttcagttca tagcccaaag gaaataatta
     4801 atctaaaaaa atatataaaa aatgcaatag aagctcaatt aaatggagag ggatattcct
     4861 tagttgaaat attagcacct tgcccaacta actggggtgt atcactagag aaatctatca
     4921 aatggatgga agaagaaata gtgccatatt atgctcttgg cgaatttaag caaagggatg
     4981 gtgaatagtt atggaaaaag agctagtatt tgcagggttt ggtggtcaag gagttttaac
     5041 tcttggtctg attgtagctg agtcagcact tgaattgggg aaacaagtaa cttggatgcc
     5101 tgcatatggt ccaactatga gaggtggaaa agcctatagt gtagtaaagt tttctgatga
     5161 gagtataggt gggcctgata tggaagaaat agatgtttta gtagcaatga ataaaccatc
     5221 tttagattat ataaatttag ttaaagaagg tggaacagta gttattaata catctgcaat
     5281 agatgagaat gtaacattaa gagaagatat aagtgtagtt aaaataaatt gtcaggaatt
     5341 agctcaaaaa gtaaacaatc caaaagcagc aaatatagta gtacttggag cattaattgc
     5401 taaaactggg ttacttgaaa aagaattagc tttaaaaact atgtgtgatt tctttgagga
     5461 aaaaggaaaa ggaaagttta atgttcaaaa tgaagcagca ttcatggaag gctataataa
     5521 cgccatttga taaattaccc ttatcttaag tgatttatct caagatttaa taatctttag
     5581 tgccaagaga aacttcatct tgagataaaa ttcacttaaa atcacaacta taaacatata
     5641 ggagcgtgaa tttatgagtt tggaaaaact tattagacca aaaagtattg ctatagtagg
     5701 agtgactgat aaacttggtt ttggaagaag tgcagcactt agtatagtaa aaagtaagga
     5761 aacagataga gtatattatg ttaatcccaa aagagaagaa ttgtttggga gaaaatgtta
     5821 taagatgata caagaagttc cagaagtagt agactgtgta gtagtatgta cgcctagaaa
     5881 tgtagttcca tctgtattaa aagattctgg agagttagga acaaaggcag cagtggtata
     5941 tgctagtgga tttgcagagg aaggtacaga ggaaggtaca gatttagaaa atcaacttat
     6001 agaaatatca aatacatacg atatgaaaat attgggtcca aactgcatgg ggttgctaaa
     6061 ttgtatagat aaggttaatc tatgggcagg aggttctaaa tgggctttag atactaaaaa
     6121 acctggtata ggaattgtag gtcaaagtgg atttataact gctgaaattg tttcaagtga
     6181 ttattttaat atatcttatg gtttttcaac aggaaatgga aatatagtta cactagaaga
     6241 tgcagtagat ttccttgtag atgatgatta tgcatcagta atagcaattt accttgaagg
     6301 tttaaaaaat cctcaaaaat ttatagatgc tttaaaaagg gcagcacaaa aaagaaagcc
     6361 agtaataata ttgaaatcag gaaggtcaga aaaaggtgct atttctgctg cctctcatac
     6421 aggaaattta gctggttcga gtaaagcttt tgaaagtatt tttgaaaaat atggagtaat
     6481 ttctgtggaa aatttagaac agtttatgtg tttagcacaa gcttttagtg ttttagatgg
     6541 taatttacct gcaaattcta attttgcagc aataaacttc tcaggaggag aaaatactat
     6601 ttgtgcagat ttagcagaag aaaatggtgt agaacttgca gaaatatctt ctgaaactaa
     6661 agaagaaatg aaaaaatatc ttccaggatt tgcaacacct aaaaatccac tagatgctac
     6721 aacagcatta ttccatgaaa aggatatgat agtaggttta ttacatacct ttgaaaagga
     6781 tgatagtgtt ggatttacta tggtaggtgc aaatataaga gatgaagaaa atgaaatgca
     6841 tgaaaccctt tgtaaagctg taagtgaagc tagagaacaa ggattgaaaa aacctgtgtt
     6901 tgcagttcct acacttgaag gaaatagata tttagattat aggactagat tagaagataa
     6961 tcaagtgcct ataatgtcat cagttggaac tacatttact tgttttaata aaatggctaa
     7021 gtttatagat tatgattatt caaaaagaac tttagaattt aaagctgtta agaaaagaga
     7081 gtcaaataat gtagtagcat tatcagaatt agatagtaag attgagatga aaaaatatgg
     7141 agtaccagtt ccaggacaag gtaatacaaa aagtattgat gaattagatg aaatcttaaa
     7201 atatattaag tatcctgttg ttttaaaaat aaattcttct gaaatattgc acaaatctga
     7261 tgttggagga gttaaaatag gaattaagaa tagagatgag gcagtagatg catataatga
     7321 gattttaact aatgtaaaaa aagcaaaacc agatgctaat attgatggta tattagttca
     7381 agaaatggta gaaagtggta tagaaattat tattggtata actaatgatg accaatttgg
     7441 accaatgtta ttagtcggtt taggaggagt atttgtagag gtatttaaag atactacatt
     7501 atatccatta cctataaatc atgatgaagc tattatgatg cttaaaaaac taaaatcatt
     7561 taagctttta aatggctata gaggaagtga gccttgtgat attgatgctc ttgcagatat
     7621 gatggtcaaa ttaggaaaat atgcttatga aaataaggat gaagtcaagg aaatagactt
     7681 aaatccagtg tttgtatatc ctaaaggaaa aggcgtatgt gcagtagatg ccttaatagt
     7741 taaatataaa tagtttataa gttttggtat tcaaccataa ttaaagcacc gactgaaaat
     7801 cacattttgt aagttagata gtgtgatttt cagtcacccc aaatttaatt taaaaatatt
     7861 ttaaaatgaa agtatagttt tgagtttaat tgtgaaatag cactgtataa acaataaaat
     7921 actacgtata aaacgaataa taaaaggtgt tttaatgata agaatcaatc ttgattttca
     7981 ttaaaacacc ttctttatta tattaaaaat ttttaaacgt tatattgtca caggtgggtt
     8041 tgttggagct gcatatgtac gattagcaaa ttcttggtca acaaagaata gaccaccctc
     8101 tccagcaagt ttaacacgtt ttatattgtc ttcacaattt gtttcttctt ctgcttgttc
     8161 agatataaac caaagtaaga attgagaagt tctaaagtct gattcagagt ttgctacagc
     8221 tgctaagttg tttattaaag ctgtaacctt tttttcatga gctaacgtac gctcaagaat
     8281 atccattgca gaagtaaagt ctgaatctgg cattgggata gcatcaagct gaacctttcc
     8341 tcctacatta tgtaagtatt tgtaaaaaaa catagcgtgg tctgtctctt ctttatattg
     8401 aacataaaac caattagaaa atcctttaag tccttctgct tcaaggtaag aagacataga
     8461 aagataaaga tacgcagaat ataattcatg gttaatctgc tcattaagta atttttccat
     8521 tttagctgaa atcattttta tacctccaaa ttttaaatgc tcttataaaa tataataata
     8581 ttaaatggaa aaatattcaa taaaagtgtt taaatatttt acatatttaa taaaaattta
     8641 tactaccatt aaaaaaatta taacttacaa tggctataga gccaattaat ggtgcatttg
     8701 ctccaaaatt tgaatgcctt acacttattt ttttatattt agaaaaactt gctgaagcag
     8761 tcaatttttt taataatgta ttttctataa atgtaccagg tatattaaag tcatagccta
     8821 ttattataga actacaatca atcaatgtaa gtgtattaac taatgcatat gatacgtatg
     8881 tacaaaattc atcaagcaaa aacatggcaa gactatcttt attatttgcc tcatctatta
     8941 acttgactaa agaaaaatct ttacagttaa ttaatgggga gttaggataa atatggctca
     9001 atgattcaat tcttttaatt agattactga cacttgtata ataatctaaa catccattgt
     9061 taccacaact gcacaatggt ccactaaaat taattgatgt atgtcctatt tctccacttt
     9121 gtcctaggtt gccagtatga agtttatttt ctagaactag accagctcca ataccattca
     9181 ttatgtgtag gtatatataa tttgatatat ttttacctaa accatacatt ttttctgcta
     9241 atgcacccga attagcatca tttatcaaaa atattggaaa atcagacatt tcttttataa
     9301 aatctactat atttaggttt gatacatttc caaagtctgg gggatttagt atggtaccat
     9361 ttatatcatc tactggacct attgaagaaa taccaattcc aattatatct ctcttgttga
     9421 ttttcattat tttagtaaac attcttttta ttatatcttt taaaacatca tttgatagac
     9481 atttagggta agtttcagaa agttgtttta caatattacc ttttaaatct gaaataatta
     9541 ccttacatat atttcttctt attaggattc cacaaataca aggcgatata ttagaaatat
     9601 ctaataaaat aggtttacga cctgatgaat gtgcatttaa agttgatgac tctatttccc
     9661 taatcatatt ttcattaata agttcagtaa ctagatttga taaagtcatt ttagtaagtc
     9721 ctgtagactt agctaaatca actctggaca taggtgtatt tgtagaaagt aatttcaata
     9781 ttattaactt atttttaatt tttacatctt tgatgttaga accttgtttg ttactcatca
     9841 taaatctcct taaataaagt taagtgtttt atttattttc aagtatacta tcaccttata
     9901 atagagtaac atttttttac ttgaaaatat cattaattag aatatatcac attatatatc
     9961 ataaaacata taaaaaaatg ttagaattaa attaaaagtg aaaagtagat tgatatataa
    10021 atagtaaata cttaattaaa aaaagataat gatagcaata attattgtta ccaagaatct
    10081 actacttcaa gtgttacctt aggtatttaa gttgatagtg gtttgcacgt aataaacttg
    10141 atgtaatata tattcttaat aatatcgata aaagatttac ttaaaaataa tatagattat
    10201 gtaaacatat aatctattta ttgtatacaa taaaatttca ttttgtaaaa cagtgaatta
    10261 aatatgtaat atagatatcg atttttatta caaattgtta ctaaatatag gtacattaga
    10321 acaaaaatct agtaaattgt aactaaaatg taaatttttc ctttaatcta tacaatatta
    10381 tggaaacttt ttgtaattta tgatataata acaaatatat aattattaaa ttataataaa
    10441 tgactatagc gtagatgtaa tactatgtta tagtcatttt tgaattaaac ttgatatatc
    10501 aagggggaag aatatgagaa caattagaag agttttagca ttaggtttaa cattagctat
    10561 atttctaata aatgtaccta atgtagatgc attaacatca gatacaatta aaggtaacaa
    10621 tatatatgag acggctggat taatagcaga taaaaagagt tatgatacag caattatggt
    10681 aaatatggat aattctatag cagatggtct ttcagcaagt ggtcttgcag gtgctgttga
    10741 tgctccaatt ttgcttgcac aaaaaaataa gataccaaat gaaacaaaac aaagattaaa
    10801 aaatgttaaa aagatataca taataggtaa agaattatca ataagtaagt cagtagaaac
    10861 tgaactaaaa aacacagggg cacaggtaac tagattgggt ggagatgaca gaataaagac
    10921 tagttatagt gttgctaaag aggtaaatgg tatcaaaaaa gttgatgaag tgatattaac
    10981 taatgcatat aaaggagaag cagatacaat aagtgctgca cccgtatcag taagagatat
    11041 agctcctata gtacttacag atggaaagag tgtgcctttt tcaacaagca atgtaaaaac
    11101 atatgcagta ggtgggagta tatcaatgag cactagttta gttaataaaa ctaatgctaa
    11161 aagacttggt ggttcagata gatatgatac aaataagaaa gttataaaag agttttatcc
    11221 agatgcatca gaattttatt tgagtgatgg atatgattta gtaaatgcac ttacaggttc
    11281 tacaattgct aaggaaaatc caattgtatt ggtgtcagaa agtagtgata agtctatatt
    11341 agcaggagca gataagatta ctagattagg ttcaataagt gacagtgtgt ataataagtg
    11401 tgtttctgct gcacaaaata atggcgattc gtctacaaaa ggtgagtcac ctatgaaaaa
    11461 tgagacaagc atattaggtc agccaacagc tagtttagaa gcgtgcttaa aatgggcaaa
    11521 atctaaaaaa gctaatgatt tatttataga gttaatacca atattatatg atactgctgt
    11581 tcaggaaggt gttaatcctg ttttggcagt agctcaatct gcaaaagaaa ctggtttttg
    11641 taattttggt ggagtattag atgcatcatt taagaatcct tgtggactta aaacttctgt
    11701 aggtggttct gatactgata agaatgccca ttcaagattt gatacttggg aagaaggaat
    11761 attagctcaa attcaacatt tatgcttata tgcaggacaa gatggttacc cactttcaaa
    11821 tccagtagac cctagacatg aaaaatcttt atttggtaag gctaaaacag ttgaaagttt
    11881 atctaataat tgggctggag gtcaatatgg acaagattta gtaagaatga tgggagaaat
    11941 tgaggcaaca aaataattga agattatcaa atatccaata aataaggatt gattattatg
    12001 aaaaagagta atatttttag tatcatattt cctacaatca taatggtgct aatgttttta
    12061 acttttgcta cagatatatt taatattcca gatattcaat ctaaaagtat ttttgttgtg
    12121 ggaatggttt tgatttttcc aatttctttt ttaatacaag ggataatttg tgtattaaat
    12181 aagactaatt ggattttatc tcttattgtg tctttagtaa cttatatagt attaatgtat
    12241 atatttttaa atgattctgc atatatatat gttttacttt atacagcttt ttatatgatt
    12301 ggacatataa cagctaaact ctattacaaa attaagactt ttaaaaatta aattgaagta
    12361 tataaaagta acattaagag aagtatcctt aatgattttc taaaatcaga aataggtgct
    12421 tctctttttt gtaaaaataa aagtatgtaa ataaaattta accattataa ttttctttta
    12481 acaaatgtat acaataaact tgttgtataa ttaagataga taaaataaag ggagggataa
    12541 tatgaattta atagataatc atattcatac taatttttca agtgatggaa aggattccat
    12601 ggaagataca ataaaaaaag ctataagtat tggtgttaga tatttgactt ttacagacca
    12661 tttagaacat gatgaagata gaggattttg cataaattat aatgactatg ttccagtatt
    12721 taataagttt aaagaaaaat ataaaaagga tatagagtta ttactaggtg ttgaagtagg
    12781 ttacagaaaa catctaaaaa atgaaataga agaaataata aattctaatc cttttgattt
    12841 tgtattgtgc tcaactcata caattgacaa tgtacctgtt ccatcaaagg cgtattttaa
    12901 aggtcttagc aaagaagatg cgtattataa atattttaat agcatacttg aaactaatcg
    12961 tgaatttgga gattacaata tatatggtca tttggattat atatctaggt atggaatata
    13021 ttcagataat agagttatat acaatgactt taaagatgtt attgatgagg tcttaaagtc
    13081 aataatcaat aatgggagtg gtatagagct aaatacttct ggttatagat atggattaaa
    13141 tgctatacat ccaaatgaag atattctaaa aagatataaa gaacttggag gatttatagt
    13201 tactgtaggt tctgattctc atagggtaga agatatatgt aaagattttg atgtggccta
    13261 tgatatgctt aagtatcttg actttaaata tgtatcttta tttaaagaaa gagaaactta
    13321 ttttgtaaat ataaaaaaag ttaaatctaa caatatagct tagagtatgt tttgaagtaa
    13381 attaatggat agttaaaaag ttaatagtta taatattgta tcctctagaa aaatactaga
    13441 ggtttttatt ttgctaaaat aactaaatag aattaaattt aaatagtata gtttatttta
    13501 aattgaatag tttattgttg atatatttta ctattatata tttttattgt gatataatag
    13561 taaaatatat taacctatct ttagattata taaattatta taaaggaggg aaattttaaa
    13621 atgtttaata aaaataaaga aaatataact ttaagtaatc aaatcaaagg aacaattcaa
    13681 aatagtttaa caaaagaagc tatattacat ataccaatga gcaattatgc ctatggttat
    13741 gatagagaaa cactacatat aagacttaga actaaaaaaa atgaagcgaa aagagtaatt
    13801 ttacgaatag gagaccaata tgtgtgggat aaaggaggtg caggaggagg aaatttaaat
    13861 gcatctggag ttggttggtc tggtggaaca aatatagtaa tgagtaaaga agtagaaact
    13921 gaattatttg actattggat agctaagtgt aagcctttaa ataaacgctc acgatatggt
    13981 tttataatag aaggtcaaga agaaaaactt ttatttacag aaaaaagaat aatagagttg
    14041 ggaaatgaaa atgatgaaaa ggaattatgt gaaataggta atttttttgg atatccatat
    14101 ttaaattata tagatatacc taaagtgcca aattgggtga aagatactgt ttggtatcag
    14161 atatttccag atagatttgc aaatggtgac ccgtctataa acccagaagg agtagataaa
    14221 tggggagcta ttccaaccag agataatttt acaggtggag atttacaagg tgtgatagaa
    14281 cacttagatt atttgagtga tttgggaata aacggaattt acttttgccc tattacaatt
    14341 ggaaaaacca atcatagata tgatacggta gattatatgg aggtagaccc tacattagga
    14401 gataaagaga ctttaaaaaa actaatagaa gaagcgcata agagaaatat aaaaataatg
    14461 ttggatgctg tttttaatca tataggatat tattctaaac agtggcaaga tgtagttcaa
    14521 aataaggaaa attctcgata taaaaactgg ttttatataa aagatatgag taaaattgat
    14581 actccaatag aacagataga tgaaaaaaat ataccttatg aaacctttgg gtgtgaaaaa
    14641 tatatgccta aacttaatac agaaaattca gaagtaataa aatatttatt ggatgtaggc
    14701 aagtactgga tacaggaatt tgatatagat gcgtggagat tagatgtttc aaatgaagta
    14761 gaccatgttt tttggagaaa gtttagagaa gaagtcaaaa aagtaaaacc agacatttat
    14821 atattgggag aaatttggca tggtagttta ccatggctaa tgggtgacca atttgattca
    14881 gttatgaatt acttgatgtc agaagccatg aaaaaattct tttgtacaga tgagataaac
    14941 gccgaagaat ttaagtatat gataaatgat gtaatggtaa gttatccaat acaagttagt
    15001 gaagttattt ttaatttact tggaagtcac gatactacaa gaatattgac ttatgctaat
    15061 ggaaatattg atagatttaa acttgcatat atgtttatgt ttgtacaaac aggatgtcct
    15121 tgtatatact atggtgatga aatcggaatg gaaggagaat taagttcaat atctgaaggt
    15181 caaagaaaat gtatggaatg gaatgaagaa aaatggaata aggatatatt tgattttatg
    15241 aagaagatta ttagacttcg taaagaaaat aaagaactta ggtctatatc aaatgaatgg
    15301 gtgttagcag ataaggaaaa tggaacgatt atattgaaaa aagaatctat ttctataata
    15361 ataaataatt catctaaaaa tataattttg acgttaccag attatttagc aaataaaaaa
    15421 gtaaaagatt tatatgaaga agaaatagtt gatttaaaag aagaactaat gttagagaaa
    15481 tacaagttca ttattttaaa ataatacaaa aagtatttat tttagatttc atgttaaatt
    15541 attatacagt ttaaacaagt ctttataaaa cataaaaaaa tatggtttac aagtgagtga
    15601 attgaaagta gattttaaat taaagactga gttagagtag ataattttat aaataatatc
    15661 ttaaatacaa gatagaaaat aaattgatta ttagtctaaa aatttcttgt agaaagagga
    15721 aataagaatg attgaagcat ttatatttga tttagatggt gtaattacag atactgcata
    15781 ttatcactat atggcttgga gaaaactagc ccataaagtt gggatagata tagacacaaa
    15841 atttaatgaa tctttaaaag gcataagtag aatggagtca ttggacagga tattagaatt
    15901 tggaaataag aaatattcat tttcagaaga agaaaaagtg agaatggctg aagaaaaaaa
    15961 taattactat gtatcattga tagatgagat aacttcaaat gatattttgc caggcataga
    16021 aagcttgtta attgatgtaa aatctaataa tataaagata ggtctatctt ctgcaagtaa
    16081 gaatgcaata aatgtattaa atcatttggg tataagtgac aaatttgatt ttatagctga
    16141 tgcaggaaaa tgtaaaaaca ataaaccaca tccagagata tttcttatgt cagcgaaagg
    16201 gcttaatgta aatccacaaa attgtatagg gatagaggat gcaagtgcag gtatagacgc
    16261 tataaactca gctaatatgt tttctgttgg tgttggaaat tatgaaaact taaaaaaagc
    16321 aaatttagtt gtagattcaa caaatcaatt aaagtttgaa tatatacaag aaaagtataa
    16381 tgagtatata gttaggagaa ttatatgaaa tgtttattta gtaatgtaga tgagtggaag
    16441 attatacaag ataagattga aatcaaagaa aatagattag cagagtccat aatgagtata
    16501 ggcaatggat atatgggtat gagaggaaac tatgaggaaa attatagtgg agatacacac
    16561 aaggggtctt atatagctgg agtatggttt ccagataaaa ctcgtgttgg ttggtggaaa
    16621 aatgggtatc cagaatactt tggtaaaatt cctaattcag tcaactatat aggtgtaaga
    16681 attttcatag atgatgtaga attagattta gcaaaatgta aagtagaaga tttttataga
    16741 gaattagata tgaaaaatgg tattttaaat cgtaggttta ttgtaaatat aaatgataaa
    16801 aaatttgaag ttagggttac taggttttta agtatatctg ttaaggaact tgcagtaatt
    16861 aaatatagta taaaatcatt aaattctaat actaaaataa aatttgtacc atatttagat
    16921 tcaaatataa aaaatgaaga ttcaaactat gaggaaaagt tttggaatgg aatttcttca
    16981 agttctttag aatataaagg tagtattctt tctcgaacaa aggagaatgc atttaataca
    17041 ccagtattca ctgtgggttc aatgatgtat attaagacaa atggtgatgt agaagctgta
    17101 tctcatgaga gtgattataa ttactgtgaa aattcgataa caatggatgt tttaaaaaat
    17161 aaggttgcaa gtatagaaaa atatgtatct ataacttcaa ctagagatta taaggaaagt
    17221 gagctattaa gaaaatgtga agacatttta aatatagaac tatctaaaag ttatgatgat
    17281 atattaaatg aacatacaaa gttatggaat aaaagatggg aaagtgcaga tgtaaaaata
    17341 aatggagata aaagtagcca acaaggtata agatataatt tattccaact attttcaaca
    17401 tattatggag aagattcgcg attaaatata ggtccaaaag gatttactgg tgaaaagtat
    17461 ggaggagcta cttattggga tacagaagca ttttgtgttc cagtatatct aggtgtagca
    17521 gataaggagg taactagaaa tcttttagaa tataggtaca atcagttaga acaggcaaag
    17581 gataatgcaa aaaaactagg gcttaaaggt gctttatatc caatggtaac atttgatgga
    17641 gtagaatgtc ataatgaatg ggagataaca tttgaggaaa tccaccgaaa tggaagtata
    17701 gtatatgcaa tatataacta tacaaattat acaggtgact ataaatatat aaaagaaaaa
    17761 ggaatagatg taataataga aatagctaga ttttgggcaa gtagagttca tttgtctaca
    17821 aaaaaagatt tgtatatgat acatggagta acaggtccaa acgagtatga aaacaatatc
    17881 aataataatt ggtatactaa ttgtatagct aaatggtgtt tagattatgc tgtagaaaat
    17941 atcttacgat tagagaaaga gtgtaatgaa tgtattgaca gaaataatgt gactagggaa
    18001 gaagttgagt tttggaaaat aatatcaaat aaaatgtatc tgccgtatga tgaggaacta
    18061 caaataataa ttcaaaatga taatttttta gataaagaat ttgtagaagt aaaggattta
    18121 ccaaaagaaa attttcctct taatcaaaat tggtcttggg ataagattct taggtcgtgt
    18181 tttataaaac aagcagatgt attacaggga atttactatt ttggaaatag atttactaaa
    18241 gaagaaaaaa agagaaattt tgacttctat gaaaaatata ctgtccatga atcttcttta
    18301 tcttctagta tttattctat tattgcctca gaaatagata acctagaaaa ggcttatgaa
    18361 ttatactcaa gaacagctag gcttgattta gataattaca ataatgatac aaatgatgga
    18421 ctgcacataa cttcaatgtc tggttcatgg ctttccattg tacatggatt tgcaggcatg
    18481 agaacatgga atgaaactct atcttttgac cctaagttgc caaaagagtg gaaaaattat
    18541 tctttcaata taaattatag agataatatt attaatgtag atgtatgtag tgatttaatt
    18601 aaaatagaaa atttaaaagg taaaccaata aaaattattg tatatgggaa aaaatatata
    18661 ttagaaaaat atataaaaat tgataagtag atttaataat atttttatta tataaatagt
    18721 taaaataagt ttatttaatt atttatctat tttaactatt ttgtgtgata gaaaactata
    18781 tattataaca atagtagtgc ctttaattaa atatatttca taaatcttca taaaatcttc
    18841 aaaatttcgt aaagaatatg tcacaaaagt aaggtatatt atattcagca gatagtataa
    18901 gggaggacaa tatttatgaa gaataaaaaa ttcattgtta taatactgat aatatcaatt
    18961 ttaataggtg tattagtgaa ggaatattca tctagagaga taaaaaagga tgatataaat
    19021 gtcagtaagt atataaaata tgctgattta gcaagtaaaa ataatgcaca agttaactgg
    19081 aagtatgtag cttccattgt tgctgtgtta aataaaaata acttaaaaaa cgttaaggat
    19141 tcacagatac aagaagtatc agatttattt gttaagaatt tttctaaaaa taataaaata
    19201 aataagttaa gtgatatttt ggatgagtta gaatttagta atagacaaaa aagattggtt
    19261 gataattata tagacaatct taaggattat ggtataaaac cagaaagatt gaagtctgat
    19321 acaaaatata tgaagtttat tgctgaaatt aaaacagaag caatacaaaa ttataaggat
    19381 tataaaatac ttccatctat aactattgca caagctataa tagaatctag ctggggcaag
    19441 tcaactttgg caaaacaata taacaatcta tttggtataa aggctgacgc ttattggaaa
    19501 ggtaagtcag tgactttaga aacgaaagaa catttagata caattataga tgataaattt
    19561 agaatatatg atgataaaaa tgaatctata aaagaccatg caaaattttt agccacaaat
    19621 aaaaggtata aaaacaatgg tgtatttgat gcaaaaacgt atatatatca agctaaagca
    19681 ttagaaaagg caggttatag tactgcgaag gatgaaaatg gcaatagtat atatgcagct
    19741 cgtctaatag aattaattca acagtataat cttcaattaa tagacagcga aattcaaagt
    19801 gaagtgtaac ttttatgtaa cataggagtg atatatttat cgaggaggtg gtttttatga
    19861 aaaaaatatt ctcgatattt ataagttgta tggttgttgt aactactatt actggatgta
    19921 gtagtggtga ggtgataaaa aatacatcac ctatcaatag tgttaaaaat ttggatttta
    19981 gttttgaggt gaaacctgaa atatttgagg tatcagttga atctggtggt tcaaaagaaa
    20041 agatatctga accctcaaaa gaaaggaaag tatcaaattt taaaaagaat aagacagagg
    20101 catcgtggac gtatcctgat gaaaatatag acataaatat aaaaaaagag gacagctatt
    20161 tagatgtaaa tattaaatct aatttagaaa aagaaactag ttttaattgg cccaaagttg
    20221 aaggggaaag ttatatacta ccactttatg agggtaagtt tataccaagt aatgataagt
    20281 attggaagga gtatctaaat gagtcaactt ataatgttat agaatctttt tcaatgcaat
    20341 tctttgcagt aaacaaggca aagtattctg tgatttatat tatcgaaaat gaatttaaca
    20401 atgtaattga atttagttct aaagacaaga ttaattttac atttaaacat gaatatccaa
    20461 gtatcaataa aaataaggag tatgggttta aaatatattt gactaataag aatattacag
    20521 atgtagcaaa aacttataaa aattatatag ttgataaagg agaatttaaa acattaaaag
    20581 aaaaatctaa ggaaaataaa aatattgaaa aactttatgg agcaccacat atttattttg
    20641 gagataagtc tatcatcaca ataaatgata ttgaatggaa taaattaaaa gaaaatttat
    20701 cagatgattt gactaattgg atgaaagagc ttttaagtat gaagtctggc aatagtgaat
    20761 ttataaaagt aattaatcaa ataaaaactc aagattatat agataaatat caaaaaaatc
    20821 aaatagtaca agcatttaat gaagttatgc agatgaatac tttttataat gaaaaagttt
    20881 ttgaaagcaa agatagagaa ataaaaaaag tattagataa aggtatatct aaattaagta
    20941 gggtagaact tatagagttt aataagaaat tattgaaatc aaaattggaa gagagcgtga
    21001 atgatgttgc tacatgggca aaatataata caactgatgt aattaatgat attaataagt
    21061 ctggtattaa aaatgcgtgg ataggatttg atgatatgga ggaaggttat atatccttag
    21121 agatggttaa aagagctaat gagttaggtt atctcgtagg gccatatgat tcataccact
    21181 ctatacacaa accaggggaa gaaaagtgga gtacagcatc atttaaagat aaatctctat
    21241 atgaaaatgc taccatagaa aataaaaaag gtgaaaaaga aactggattt caaggagaag
    21301 gaagaaaact aaatcctaca ttgtctttac caagtgtaaa agagagagta caaagtatat
    21361 tggatgaagg atatgaattt aattcttggt ttatagattg tgatgctaca ggagaaatct
    21421 atgatgatta ttctcaaaat catataacaa cacaaacaca agatttaaat gctagacttg
    21481 aaagaatgtc atatataaga gaccatgcaa atatggtcat aggttcagaa ggtggaaatg
    21541 atttttcaag tagtactata gcatttgcac atggtattga gacaccagca tttgcttggg
    21601 tagataaaga tatgagtaag aataaaggca gtgaatatta tgttggtaaa tactattctc
    21661 caacaggagg agtaccagaa atattttcaa agcagatacc tgtaaaagaa aggtacaaaa
    21721 aaatatttat agacccaatt tactctctac ctttatttag attagtttat aatgattctg
    21781 tgatatcttc ccatcagtgg ttatggggaa ctttgaagat taaggatgaa gttggaagta
    21841 gaatgatgaa agaagtatta tataatacac cacctatgta ttatatagat agaactgaat
    21901 gggaaaaaca taaagatgaa ataatctcaa acaataaagt atggtcagcg tttaataaga
    21961 aagctattaa aaaagaaatg acagacttta aatttatttc tgaggataga ctagttcaaa
    22021 tgactgaata tggagaagat ttaaaggtat tagttaattt ttctcaaaaa gacattaagt
    22081 acaatgatga agtcataaaa gcaaattctt tggtaatata tgatgctgat agtaaagtca
    22141 tatataaacc ggaataatag atttaatgaa aataaagatt gtttaaattt caagtaaaaa
    22201 ataatataat attcatttag tttgtattaa agtagtgact atattaatct ggataattag
    22261 tatagtcact attatttata ttctttccta aaagtaagta gtaactgaat cgtgaataat
    22321 caaaaatggt aatttgaaat agtttttatg aatagtatta atatatagat atagggagat
    22381 tgatgtgatt atgtatgaaa ataataaggt aatatataat tttgaaagat gtgaaaagct
    22441 agaaaatgct ctttatttat ctcaatttaa tacatataaa aactttaagt taacttctaa
    22501 aaaaactgta ataaactcat tagatacatc tacttataat atgaatattc atagcataag
    22561 tctcgttgat actatggaat gtatatcgtc agaagatgaa caaatgacag gaaagaagtt
    22621 gattttagtt ggcgatatag atactgatgt aacattagat tatgcaggca ataaaagaaa
    22681 tagaaattct agtaaaaaaa atacatttaa attaaaaata ccctttagta catttataca
    22741 aatgcctaga aagatagaaa ataaagataa aattaattta aagtatctta tacaagatat
    22801 tacttcatct attttagatg ataatttatt tattagtgtt actgcaatta taagttatga
    22861 aaatagttcc aagatagagt aatctttaaa ttatagaaag gtggatgcaa tgagaataac
    22921 gaaaactact tttaatgatg tattttcaaa tgatgcaaat ggaagtatta taaaagtaga
    22981 aaaatatgtt gataaaaaat gtgcatctgt atttgagatt ttgacctaca ctattattgt
    23041 tacaaataca agtaggtaca aaactggaaa tatatttttc aaagattata tttctaaata
    23101 tatagagttt ataaataata ctgtaaaagt taatggcatt ataaaacgtg gattagaccc
    23161 acagcaaggt ttttacatag ggagaataga tgcaagttgt aaaaaaataa tatcttttaa
    23221 gagtgtagtt ttaccaaatt ctgcacatag gattataaag aatagtgcta atatatacta
    23281 ttattataag tgtaatctag ataaattccc aacaagaata tcaatagaaa gtaatagagt
    23341 ttatactaat gtaaataaaa ttgtattcaa acaattgaat ataagtaata ctttaaaagc
    23401 tcctaaaaat ctaagagata tattaaaagt aaatgtaaat agtaaaatat ttgatgttaa
    23461 acctatgaaa aatcttataa acaaaggtag taatttgaaa ttatgtaact cgatagtttt
    23521 tgggtgtata gaagttgaaa tagactatag ttgtaaaaat aggagtaatt ttaaaacagt
    23581 aaataagttt aatgatatta aaaaagataa gtattataaa aatgatatac aaaataacaa
    23641 tgaaaatata aacgattgta aaaagcaaat aagttttggt gataataagg taaaaaaaat
    23701 aacaaaaact tttggatttt cttgtttcat ttgttctcca gttggaatta tttatgaaga
    23761 tatgaaaaat ataaatataa aaattgaaca tacatcaata aatgaattaa atccagaaga
    23821 attatttgta aatacatcac ttttattgta ttattaagta ttttaaagct aaaacttaaa
    23881 cttattaaaa tag
//
LOCUS       DAELSY010000055        23681 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.055, whole genome shotgun sequence.
ACCESSION   DAELSY010000055 DAELSY010000000
VERSION     DAELSY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23681
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.055"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            297..1568
                     /gene="serS"
                     /locus_tag="KPG09_001863"
     CDS             297..1568
                     /gene="serS"
                     /locus_tag="KPG09_001863"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG0966474.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKHKLNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            1936..3441
                     /locus_tag="KPG09_001864"
     CDS             1936..3441
                     /locus_tag="KPG09_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG0966475.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            3555..4253
                     /locus_tag="KPG09_001865"
     CDS             3555..4253
                     /locus_tag="KPG09_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG0966476.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            4538..5626
                     /locus_tag="KPG09_001866"
     CDS             4538..5626
                     /locus_tag="KPG09_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966477.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            5865..7055
                     /locus_tag="KPG09_001867"
     CDS             5865..7055
                     /locus_tag="KPG09_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0966478.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIEYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYTIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            7146..8648
                     /locus_tag="KPG09_001868"
     CDS             7146..8648
                     /locus_tag="KPG09_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG0966479.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            8720..9976
                     /locus_tag="KPG09_001869"
     CDS             8720..9976
                     /locus_tag="KPG09_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG0966480.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            10148..12922
                     /locus_tag="KPG09_001870"
     CDS             10148..12922
                     /locus_tag="KPG09_001870"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG0966481.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAQALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRSMNSNSKN"
     gene            complement(12987..13172)
                     /locus_tag="KPG09_001871"
     CDS             complement(12987..13172)
                     /locus_tag="KPG09_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG0966482.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEVYKILQKLDIDFERVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            13544..14722
                     /locus_tag="KPG09_001872"
     CDS             13544..14722
                     /locus_tag="KPG09_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG0966483.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMFTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIVSIMIKIWLSRFNKFVGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKDMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     regulatory      14848..14933
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            15046..18000
                     /locus_tag="KPG09_001873"
     CDS             15046..18000
                     /locus_tag="KPG09_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG0966484.1"
                     /translation="MKKGNKKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEDNSTDNKKPEQTSEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQTPEGEKSIDNKKPEQIPEEDKSTDNKKSEQALEDEKPL
                     DNKNTEKTPEEDNLLEDENLLKVLEEELNEDYGFVVKINNNTIETESMKKISFNLTYT
                     PTSKGIQAGDSITFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGK
                     IGLEAYVKKVDEDTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEG
                     RKSTTVFMPVKNKDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEV
                     SDFNIEQSKNSISVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQED
                     DATVSIFDRGDNYLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGM
                     RLTTSGEDGHDFRVVEVPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTI
                     NLSKEEVESKFFIAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKK
                     EVDKKVLNSSDNQIVKYKINMSTYGVYDAGQVNLLDEVNPSLKISNIKYSDNLELKKE
                     AGDGKNTIRLVNKYEFKQIKEGEPVQSWVTFDADFTNVKVGETIQNVAQINGSSPPGV
                     ETTKQGYAFEAKKVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGVIQSSVKKPGI
                     YYLVEVMAPSGYEKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPVPPNTDEP
                     IVNPPVPPSTDKPRKPSNPSDTEDTIVINPPVPPSEDIINPPIPEILNPPVPPSEEVI
                     ETPVKQIVPIPEVVKPSVSEEKNNKAKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIV
                     GLVVLRRNKNTN"
     regulatory      18186..18271
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            18371..19033
                     /gene="zmp1"
                     /locus_tag="KPG09_001874"
     CDS             18371..19033
                     /gene="zmp1"
                     /locus_tag="KPG09_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBG0966485.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHAYSTTIQQNKDTLSQIVVF
                     PTGNYNKNEANAMVNRLANIDGKYLNVLKQDNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAEAFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     gene            complement(19151..20113)
                     /locus_tag="KPG09_001875"
     CDS             complement(19151..20113)
                     /locus_tag="KPG09_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG0966486.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHIFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVLSPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(20283..21473)
                     /locus_tag="KPG09_001876"
     CDS             complement(20283..21473)
                     /locus_tag="KPG09_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0966487.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            21779..22225
                     /locus_tag="KPG09_001877"
     CDS             21779..22225
                     /locus_tag="KPG09_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0966488.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEG"
     gene            22227..23321
                     /locus_tag="KPG09_001878"
     CDS             22227..23321
                     /locus_tag="KPG09_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBG0966489.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVIKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMKMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            23409..23525
                     /locus_tag="KPG09_001879"
                     /pseudo
     CDS             23409..23525
                     /locus_tag="KPG09_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
BASE COUNT         9403 a         2703 c         4054 g         7521 t
ORIGIN      
        1 ataaaagtta ataactatac aagattgata aagaaaaagt caaaatagat aattgattag
       61 caaaatagat ataattataa tgaagttaaa tctagcaata ttctcctaaa attaaatttc
      121 atttatttaa ttaaatataa tttgacaatg gacaaaaaag tgagtaaaat ataaataaaa
      181 taaaaataac aggttcggat aaagaaaaag gtttcttaac ctcaagcata aagtatatta
      241 tctaagttat ttgagattaa gagataaatc caatcttagg tactgaggag gaaaaaatgc
      301 ttgatataaa aagaataaga gaaaatttag attgtataaa aaaagcaatg gaaagaaggg
      361 gcgaaaaaga ctttaacctt gatgaagtag ttaagttaga tgatgaaaga agaaagattc
      421 ttcaagaagt tgaagttatg aaacataaat taaatacagc atctaaaaat ataccaaatc
      481 ttataaaaga aggtaaagat gtagaaaatg aaaagattaa actaaaagag ttatctgata
      541 aaattaaggt aatagaccaa aatcttaaag aagtagaaga taagatggaa tacctattaa
      601 tgagaatacc aaatgtacca catccagaag taccacaagg tgaaactgat gaagataatg
      661 ttgaggtaag aacttgggga aagactacta cctttgattt tgaatctaag gctcattggg
      721 aaattggaac agaattaggt atacttgatt ttgaaacagc gagtaaaata actggttcaa
      781 gattcactct ttacaaagga ttgggtgcta gattagagag agcattatta aatttttact
      841 tagacacgaa tacaaaagta aatggatata cagaagtcat tccaccattt atggcaaata
      901 gaaatagttt tttaggtaca ggacagttac ctaagtttga agaagatatg tttaaaatag
      961 aaggtctgga ctattttatg attccaacat cagaagttcc tttaactaat atacatgcta
     1021 atgaaatact taaatttgaa caattaccaa taaattatac agcttatact ccatgtttta
     1081 ggtcagaagc tggttctgct ggaagagata caagaggttt agtaagacag catcagttta
     1141 acaaagtaga aatggttaaa atagttgcac cagaagaatc ttacaatgag ttagaaaagc
     1201 ttactaacaa tgcggaaact atgctacaat tattgaatct gccttataga gtagtaaaga
     1261 tatgtacagg tgatttagga tttacggctt cttttaaata tgatgtagaa gtttggatgc
     1321 caagttacaa taggtatgtt gagatttcat cttgttcaaa ctgcgaagat ttccaagcta
     1381 gaagagcagg aataagattt aaaagagata aagattctaa agctgaatat gtacacacat
     1441 taaatggctc tgggttagct attggaagaa gtgttgcagc tatattagaa aattatcaac
     1501 aagaagatgg ttctgtggtt gttcctgaag ttttaagacc atatatggga gtttctgtta
     1561 ttaaataaga gttaaattat gtatttttaa gcaatacaat aatatagaag taataaaaaa
     1621 taattaatat aaaagtgata caataatata gaagtaataa aaaaatagtt aatataaaag
     1681 tgatacaata atatagaagt aataaaaaat agttaatata aaagtgatac aataatatag
     1741 aagtaataaa aaaatagtta atataaaagt gatacaataa taatagaagt aataaaaaaa
     1801 aataattaat ataaaaatgt gtacaaacta atgaatattg atatagtaag ctatcatatg
     1861 aaagagtatg tacacatttt ttttactaat ttttcacaat atgaacataa ataatttaca
     1921 ggaaaggaat cttttatgaa aattcaaaat actgagtggg gatatattga atggaaacat
     1981 acttatgatg aaaataatcc aaaacaagct atgaatatat acattgcagt aactatgcct
     2041 ggtaagaaac attttaatca tgttcattat ggtcaagagc agatgatata tattttagag
     2101 ggagagggtt tgtatattat aaatggagtt tggaagccat tttatcaagg tatgattttt
     2161 tatatagaat ctggtagcac acatgaaact ataaatacag gcgatagaga aataaaagaa
     2221 cttattgttt caaataatgt agatgatgta ggagagagtg aagttataga tattaatcct
     2281 aataattatt taaagaagac cttgataaat tatagtgaaa gtacattaaa tttgtatgca
     2341 gcagtagaat caattcgtgg tcaatttata gacccgttta aaattccatt aattatatat
     2401 gatgattcat ggaatatagt tttaaaaaac ccttattttc cacttttttg ttttgaaaaa
     2461 tgtaatccta tgaaatttcc tcaaaattgt gattgtatga atcaaaaaag tagcaatcaa
     2521 tttgtatgtg aatatgggat aactatatat aatataccaa tactgtacaa aagcaatagc
     2581 ataggtgtta taagaggagg gtatgtacta ttatctgatt taaatttaga tacagagcat
     2641 aataatttat atgatattcc agagggagct gctagaagta taaaacgcct cttgaaacaa
     2701 atatctaaaa atataataaa tttttgctct tttaatgata taagaaagga tttacaagaa
     2761 aaagagaaga ctatagcaag aacatatcat tatggagagc aacttgaaat gaatttaaaa
     2821 gtagctcaag atatggttac taatttaaga ataaatcatc actttttatt taatacatta
     2881 aattcaatgg caagcatagc actagatgat ggtagttatg atttgtatag tgcaataata
     2941 gatttatcaa gaatgtttag atatacaatg aggtctgatt taagatttgt agaattagaa
     3001 tctgaaatat tatacattaa aaattatttg aatttacaga aattacgtta tggagatgca
     3061 cttaaagtaa aatatcttat tccagagaag ttatataacc tttctgtgcc ttttaacttt
     3121 attcaaccta tcgtagagaa tgcatttaca catggattta gagatattga cacagaaaag
     3181 cgaattgaaa taattgcaag attagattct cagtatgcta ttatagagat tcataataat
     3241 ggaactatct tagatggaaa taatatagac aaaataaagg caggtattcg tagtaataat
     3301 ggtcatggtt tgtctttaat atatacaaaa ttcacatctg cttatggaaa taattttgat
     3361 atggatataa agtcgagtga taatgaagga acttacataa tgattaaaat tcctattgaa
     3421 aattataagg agtatgtata gaacttgtga aaaattaata agttttataa atgttaagaa
     3481 atatattaga tgtttttatg ttgttttata tatcttactt ttttatatta ttaagctaaa
     3541 agggaggtat tctcatgaga agagcactaa taatagatga tgaagaagct gttctaaaaa
     3601 taataacaca ttttatagaa atgaaagata tgccaataaa aattgttggt aaagcaacat
     3661 ctggtgatga agcagtagat aaaatcattg gtttagagcc agacatagtt tttatagata
     3721 ttcaaatgcc aatatacaat gggttagaag taatagaaaa aacatctcat ttatctaaaa
     3781 aaataaactt tgtagtaata actgcattta actattttga atatgctcaa aaagctttga
     3841 gattaaatgt aaaagatata ctccttaaac cattggatat gaaagaattt actaagtctg
     3901 tagaaaaaat tattggatat caatatacca ataatgattt attaaatgag atactggaat
     3961 atataaatct aaactatagc aatgacttaa agttaaatga ttgtgctaga ttatttttaa
     4021 ccaatccatc taatatatct aggattttta aaagtaattt aaatacaact tttatatcat
     4081 atctaaatca tataagaata gaaaaatcta ttgaattact tgaaaatact acaaaaagta
     4141 taaatgagat tgcagaaata gttggatata atagcctaaa taacttttat aaaaacttta
     4201 agatagaaaa aggaatgact ccaaaagttt ataaactgaa cactaaaaat tgaaattgat
     4261 aaataaaaag tcaatataga taattgtttg agtgtgtctg aataagtata ctttaattat
     4321 gataaaaatt aaagcgcttt aaacatattt aataatatta aaccttataa aagctgtacc
     4381 tatttatata aaaaaatata tataaataaa aagaaaaaga tatacaaaat aaaaaaagtg
     4441 aattaaatac tgtttcaaaa agacagaatt ttcatttaat tcactgagag tttatatttc
     4501 ttaaaggaag gtaactttat aatggaggta atttaatatg tcagacaaag tgaatatgaa
     4561 aaatattatc tgtattgcag gcgcttatct agcaacagca attggttctg ggtttgctac
     4621 tggtcaagaa attctacaat tttttgcctc acaagggtat atgggtttaa ttggagcatt
     4681 aatatcaatg gtattgtttt catttcttgg tgctgaagtt ataactagag gtagagaatt
     4741 aaaacttaaa gaaccaacaa gaatttacac tttttattgt gggaagattt taggaaaatt
     4801 ttacgaatgg tttagtccaa tatttttatt tgggattctt atagtaatga tttcaggtgc
     4861 aggctcaact ttaactgaat actatggtat aaatccttac ttaggaagag tattgatggc
     4921 tgttgctact ttaattacag tgtctatggg tttagaaaaa ttagtaagca tacttggacg
     4981 tattggacca gtgatagcag tatttacaat tgctgttgga ttaataagct tgtttaagca
     5041 ttttgatggt ttatctatgg ttggagaagc tatgaaaaat attgaagtta caaaagcatc
     5101 agaaagtcca atcgtttctg gtgtaatata taatacatta aatattattg taatgattgt
     5161 ttttttaaca ggaatgggag cttctattga aagtaaaaag gatgcttttt ggggaggttt
     5221 attaggtgga atagtgttta tgactgctgg tatagttatg tatttggcta tgatttcaga
     5281 tataggaaat ttgtatatga aagaaatacc atcactttat ttagcagata atatatctcc
     5341 tattgtaggt gtatgttttt cagttgcatt aatattaggt atctatacaa cagctgttcc
     5401 acttttatgg tctgtaacca atagaattgt tgaagatgaa catcctaaat ttaaattggt
     5461 aacaacagta atagctattt tggcttgtat aggtggtttt ttaccgtttg gtaaattagt
     5521 aaacatttta tatccatata caggttatat ggggctttta attttagtct gtatggtgta
     5581 taaagcaatt acatgtagaa aaaaatcaac acaaagtcag gaatagagtc acctattaat
     5641 agtttttatt taaagaaatt ttttatataa tccgaattta aaaagtcaaa atagttaaat
     5701 aaattgcaaa acatgataat atatttaatt aaaataatat agtaaaatat aaatataaac
     5761 taaaattaaa tttataaaaa atatctttat aaagattatt aacttatata atttgtagtt
     5821 aaattataaa aaataaaaaa ataaaatttt ggagaggtgt tagtatgcat gaacttattt
     5881 taatctcaga aaagtataaa gaagatattt tagatttttt tacagatatt catatgcacc
     5941 ccgaattaag ttttaaagaa tttaggacta caaaagctat aaaagattta ttagtaagtt
     6001 tagacataga aatattagat ttaggaatgg aaacaggagt tgttggatta ttaaaaggga
     6061 agtatgatgg tcccacagta gcactgagag gagatattga tgcattgcca atatatgaag
     6121 aggttgatat tgagtataaa tccagatatg atggtataat gcatgcatgt gggcatgata
     6181 tacacacatc ttgccttgta ggatgtgcat atgtattgtc tcatattaga gatagtcttc
     6241 atggaaatgt aaagtttata tttcaaccag ccgaagaagt caataaaggt gcgaagatgc
     6301 tagtggaacg aggagtaatg gaaaatccaa aagttgatgc tatatttgga cttcataatc
     6361 atccagatat accttgtgga aaaataggtg ttaagttagg tgggttaatg gcagcagtag
     6421 acactattaa gatagaagta aatggatttg gaggtcacgg aggtatacca aacagaacaa
     6481 tagaccctat agtagcatca agtgcaatta taatgggtat tcaaaccata gtaagcagaa
     6541 acataagtcc attagaatca gctgttatat caataggtac tataaatgga gggactgcaa
     6601 ataatgtaat ttcagaaaag gtagatatga caggtacatg tagaagcttt agtaatgaag
     6661 tacgaaagaa aatatcagaa aatttagaaa atatagtttg tgagatagct cgtggatatc
     6721 aagcaactgc aaagctagat tatttatttg atttaccagc tgttataaat agtaaagaga
     6781 tgtacacaat agcttgtaag tctgtgtgtg atttgtattc agaagatgct atagttgatc
     6841 caataccttc tacaggagga gaagattttt ctatatttat ggaaaaggcc ccaggatttt
     6901 tttactggtt aggggtaggc aataaagaac aagattgtat atatcagtgg cataatccta
     6961 aatttaaagc tgataaaaat tctatattgg taggtacaaa tgtactatgt cagtctgtta
     7021 taaattatat ggataaatta aaaaataaaa tttaagatag agcattctgg agataaagtt
     7081 tatgaggggg tattagtttg aggaaaatta attcacagga aaaaaacaaa gatagtggat
     7141 tattgatgag ggcactaaat gttgtagaga gagcaggaaa taaacttcca gacccagtaa
     7201 ccatattttt attactatgt ataattgttg taatattgtc agcagttata tctaatttag
     7261 gagtagaaga aatacatcct tctacaaaag aagttgtaaa ggtagtaaat ctactagaga
     7321 aagagcagat tcaatcatat ttaggtagca tagttacaaa cttccagtca tttgctcctt
     7381 taggattagt actggttaca atgcttggag caggtgttgc tgagaaatct ggatttatgg
     7441 aagtgttaat gaaaaagggt ataagtaaag ttccacaaaa acttgtaact gtagcaatcg
     7501 tatttgcagg tatgttatca catacagcag cagatgtagg atttataata ttaccaccac
     7561 ttgcagcact tgtgttttta ggaattggaa gacatcctct tgtaggtatg tttgcagctt
     7621 ttgcaggtgt tgcaggggga tttgcagcaa atgttatgct tagtactaca gatgtattgt
     7681 tagctggatt tacaatacca gcagctcaaa tgatggaccc atcttaccaa ggcaatccag
     7741 caatgaatct ttattttgct tttatatcag cgatagtttt aacttttgca ggagcatttg
     7801 taactgaaaa atttatagct cctcgtttta caaaatataa tgattcaaca aatgataagg
     7861 atatacagga attgagtgaa cttgaaaata agggtataaa atacgcttta ttatcattgt
     7921 tagttgtagt agtagtgata gttgccttat gtataggtga caatgcattt atgaaagatc
     7981 cagagactgg ttctatacta tctagtaatg cacctttaat gaaaggtata gttcctataa
     8041 tcactattat atttttaact ccaggattag tgtatggaaa agtaagtaag aagataaaaa
     8101 gtgacaaaga tttagtatct atgatgggta gtagtatgag tgatatgggt ggatatatag
     8161 tcctagcatt tatagcatcc cagtttatta atttatttaa tcttagtaac ttaggaacaa
     8221 ttttatcaat aacaggagca aaattactag cagagtcggg gatacctagc tatggattga
     8281 taataggatt tatattgcta tctggattta tcaatttatt tgtaggaagt gcttctgcaa
     8341 aatgggcgat tttagcacct atatttgttc ccatgtttat gctactagac tttaatccag
     8401 cattaactca aatagcttat cgtattggag atgcttcaac aaatccaata agtccacttt
     8461 ttccgtattt tccagttata ttggcctttg ccagaagata tgataaagat ataggtatag
     8521 gtactgtcat atcgaatatg attccatact cagtggtatt tacattaata gaaataataa
     8581 tattgttact attcatgggt attggcatac cattaggacc tggtggagga atatcttacg
     8641 ttttatagac cattataata aatttatcaa tgaagattaa actaataaaa tatattcttt
     8701 aattaagagg aggattatta tgttaaaaac aacaaaaatt tcagacccag agttatacaa
     8761 gattgtagct gatgagttgg ttagacaaga acacaatatt gagatgatag cttctgaaag
     8821 tacagcacca acagaagtac ttgaattaag tggatgtgta tttacaaata aaacagaaga
     8881 aggctatcca ggggctcgtt ttcaagcagg ttctgaagaa gcagataaac ttgaaaccct
     8941 agcaataaaa cgtgcaaaag aagtatttgg agcagaacat gtaaacgtac aaccgtattc
     9001 aggttctaca gctaactatt gtgtatattc atcaatatta aaaccaaatg acactgtttt
     9061 aagtatgcgt cttgaccaag gtggacactt gacacatggt agcgcagtta attttttaca
     9121 tgacatttat aaatatgaat tttatggggt agacccaaat actggacgta tagattatga
     9181 tgctcttgaa gcaaaggcaa aagaatgtag accaaaactt ataattgcag gggcaagttc
     9241 atatccaaga ttaatagatt atgaaagaat atcaaaggtt gcaaaagaag taggagctta
     9301 ctttatggta gatatggcac atgttgcagg attagtcgca gcgaaagtaa tacctagtcc
     9361 tgtaccttat gcagattttg tttcttcatc tacgacaaag actttctgtg gaccaagaag
     9421 tggtattgta ctttgtaaag cagaacatgc aaagaaactc gataaaggtg tttttccagg
     9481 aacattaggg tctatacatc ttaatacagt tgcagcaaaa gccttttcac tattatattt
     9541 aagtacagat aaatttaaaa agataatgga gcaagttgta gtaaatgctc agactctagc
     9601 ttctgaactg atttcacatg gttttagtat tgtaagtgga ggtactgata atcatatagt
     9661 aatggttgac ttaagaagta agaatcttac aggaaaacaa tttgaaaaag ctcttgaata
     9721 tgtaggaata acagttaata aaaatgttat acctgacgac ccacaatcac catttgtaac
     9781 tagtggtgtt cgtataggtc ttacatcaat atctcaaaga ggtctgaaag aaaaagaagt
     9841 tatacagata gcaggaataa tgaataaggt agcagaaaat attgataata aagaagtatt
     9901 agatgaatgt aaagcagagg cacaagaact tatatctaag ttcccattat atccagaagg
     9961 gtattttgaa gattaaatat tttgatttgt atagctcaca atttataaag cctgtaacat
    10021 aaaatttaat aatatataac cactataagg tgttgattaa tcaacacctt attttttttg
    10081 aaaagtaata tacaagctat cataagcata taattcctta tagataatta acaaggggga
    10141 taaagttatg aggtattata ataaacctac caaagaagta ttaaaatact taaaaacaaa
    10201 tccagaaatt ggacttgatg ataatgaagt agaagaaagg aagcttagat atggattaaa
    10261 tgaatttact ataaaagaag gtagaacatt ttgggacgag ttgggagaaa gtttgacaga
    10321 acctatgata ttaattctca taggagcagc agtaataagt tcttttgtgg gagaattaca
    10381 tgatgcatta ggtattttag gggctatttt tattggaatt tctataggca taataactga
    10441 aggaaaatct aaaaaagcag ctcaggccct gtcaaaatta acagaaaata tagaagtaaa
    10501 agtacttaga aatggaaaga taattaaaat atcaaaaaat gatttagtac caggagatat
    10561 tgtatatata gaaactggag atatgatacc agcagatgga agacttatac agtctataaa
    10621 tttgaaactt agagaagata tgctaacagg agaatctgat gatgtagcta agaatgctga
    10681 tgctgtatta gatatggaag tagtttattc taaaacagaa ataatcgaac aggatgcaat
    10741 acctgcaaag caagtcaata tggtatttgg agggactctt gtagcatatg gtaggggcat
    10801 aatggttgtc actcatactg gtgataagac agagatgggt aagatagcac agaatttatc
    10861 aaatgaagac cagcaaactc cacttcaaat taaattaggg aagttgggtg ctaaaatagc
    10921 aggaatatca ggcgtaatag ctactttatt gtgtatgttt atgattatac agatgcagag
    10981 aaaaggtatg ttaattttag atacaagcag tatattatct ttcttacaat cattagaacc
    11041 tgctaaaaat gcatatatgg tttgtattgc attaatagtt gcaactgttc cagaaggttt
    11101 gccaactatg ataaatatta ccttagctat aacaatgcaa aagatggcaa agataaatgc
    11161 attagtaaca aagaaagaag cctgtgaaac tataggttct gtttctgtaa tatgttccga
    11221 caaaacaggg actttaacac aaaacaaaat gatggtagag gtagcatatg ttgatggtaa
    11281 atatataagt ggaggagagt atcaaagtaa cagctatttt gaacaaaact gtatagtaaa
    11341 ttcaactgca gatatagaaa aagaagataa ttcatttaaa tatataggaa gtgcaacaga
    11401 gtgtgcatta cttttatatc ataatgataa gaactataat gaaatgagaa aacaaacgta
    11461 tttgatatca caaataccat ttagttcaga agaaaagaag atgtcaacat taattcgtca
    11521 agaggattca gatatattac tttcaaaagg tgctccagaa gtattattaa aaaaatgttc
    11581 ttatgtacaa caaggaaaaa atattgttcc aataactcca aaagtagaaa aaagtatttt
    11641 agatgagatt aagaagttac aaattaaatc tatgagaaca ttaggatttg cttataaaaa
    11701 aatgagtaat tctaaaacag aagttgcaat gacttcagaa ggtgaattga atttaatagg
    11761 aaattcaaga agttatatga aggaagataa tttagttttt agtggatttg taggaatagt
    11821 ggacccattg agagaagggg tcaaagattc cattgataaa gcatttaatg caggagtaga
    11881 tgtaaaaatg cttacaggag ataatataaa tacagcaacg gctattggaa atgaattagg
    11941 attattaaat gatgggaaaa aggcagttga agcaacatat atagatgtat tgacagataa
    12001 agaactgaga gaagaaatta aaggaatatc tattgttgca agaagtaaac cagatacaaa
    12061 aatgagaata gtttcagcgc ttcaaaaaag tggcgaagtt gtagctgtaa caggtgatgg
    12121 tataaatgat gccccagctc ttagtcaagc agatgttgga attgcaatgg gtatttcagg
    12181 aacagaagta tctaaaaatg cagcagatat aatattgact gatgatagct ttagtactat
    12241 agtagaggga ataaaatggg gaagaggtat atatgagaac tttcaaagat ttatacagtt
    12301 tcagcttact gtaaatatag tagcatttat aattgctata atatctcaac ttactggtaa
    12361 ggatatgcca tttactacaa tacaattatt atgggtaaat ataataatgg atggtcctcc
    12421 agccctagca ttaggattag aacctgttag agattatgta ttgaaaagaa aaccaataaa
    12481 tagacattct ggaataatag ctaggtctat gtttgtaaat attattataa atgctattct
    12541 tataattaca attgtattta ctcaatcagc ttttaatata ttgggagcta catcagaaga
    12601 acaaggtact gttatatttt ctttatttgc gttcagtgcc ctttttaatg cattaaattg
    12661 tagagaattt ggtttaaata gtacaatacc aaatttcttt aaaaataaac tggcattgca
    12721 aataatagta gttacaggga taattcaaat aatatttaca caagtgttcc agagtttctt
    12781 taattctgtt tctttggact ttgatatgtg gataaaaata atactatttg catccactat
    12841 attattgtca aatgaatttg taaagctaat acttagaaca atgaaaaata gtagaagtat
    12901 gaactctaat agtaaaaatt aattcttatg aaaccgcaaa gtactatata ttgtaacttt
    12961 gcggtttttg tttgtttaat gttgttttat attaccattt tcatatttaa ttttttatta
    13021 atctgaacac aatcttccat tgtaattgct tcatctgtgt caacacgttc aaagtcaata
    13081 tctaactttt gtaatatttt gtatacctct ttttgcaatg gagtatcaaa tttaataggt
    13141 gattttgtgt aaatatcact gataaaaaac atgatttatt tcattccttt ccgtttaaaa
    13201 tcttatagtt atagtgtatc ataaggttat aaaaacataa atatagtata aatttaaaat
    13261 atgtaaagac aaaacaaaaa aatcatattc taagtaatac ttgggaaaaa tgatattatc
    13321 ataataaata atctttgtaa aaacttatat agaatctatt aaacagacac taagccataa
    13381 aaactttacg aaatataaaa tttgtatcta aatagatata tagaataata ttttactgaa
    13441 gtagagtgat ttttaagaat tttatatata aattatgtta tcataaataa agatatagat
    13501 tataaaattt gttaataaaa taaagggcag ggaggtaagt ttcatgtttt ccaaaatcct
    13561 agtcaaaaca tttatcagag atagcgaaaa cgttcaaaat acagatgtta gaaataagta
    13621 tggttatgta gctggtgtag taggtatatt gtccaattta cttttatttg tgataaaggt
    13681 attcatagga atgttcacat ctagtatagc tataatggct gatgcattta ataatctttc
    13741 tgatatggca tcttcagcaa taactatgat aggttttaaa ttagcaagta agccagctga
    13801 caaagaacat ccttttggtc atggaagaat agagtattta tcagcattaa tagtagcatt
    13861 tatggtgatg ttggttggac ttcaatttgt aaagtcatct atagaaagga tagtaaatcc
    13921 aattcctgta aaatttgaag taataccact tatactttta attgtatcta taatgattaa
    13981 aatctggttg agtcgtttta ataaatttgt gggaaataaa attgattctt cagccttaaa
    14041 agcagtttca cttgatgctt taggagatgt atttacttca agttgtgtag ttatatcatt
    14101 tattgtagct agatttacta attttcctat agatggatat gttggtatcg tagtatcatt
    14161 agtgatactt tatgctggtt tctctcttgt aaaagatact ataaatcctc ttttaggaga
    14221 agcaccggat gaagaaatgg taaattctat tatagagcta ttgttatctt ataaatatat
    14281 aataggtaca catgatttaa ttattcataa ctatggagtt ggtagatgta ttgcatctat
    14341 acatgcagaa attccatcca acatagatat aatggaaata catgaaatta tagatactgc
    14401 agagagagaa atatctgaaa aattagatat atatctagta attcacatgg acccaatatg
    14461 tcttgaagat aaagaagtta tgtcagccaa aaaagaactt gaagaaatac ttaagaaaaa
    14521 tagtcttgta aaatctatgc atgattttag aatagttgga aaaggaagca agaagaattt
    14581 aatatttgat atagtagtta atccttccga attttccaaa gacatgtcag aggatgactt
    14641 aaaagaagat ataacaaaat tagttaagga gataaatcct gaatataatt gtgtaattgt
    14701 tgtagataag gattttattt aattattaat taatctcata aaaggtaaaa aatgtaaaaa
    14761 agatagacaa tagtatagag gcataatata atagaagtat ataattgtat ttttaatata
    14821 aataaagcaa ttttaattgt ttttatgaaa tattatagag atgttgaagt atattctatt
    14881 attgggcacc ttatggatat actgagtcag tggtgcaacc ggctatgaat ataaatttat
    14941 ttattttcat agcttttttt atataatttt ttagtaaaaa ttaatttata tacgatatta
    15001 atggaagatt ataaactata gataacctag agggggaaaa tttatatgaa gaaaggaaat
    15061 aaaaaggcat tattaattag tttaattatg attttaagta tggtggtgtc tacaatatat
    15121 ccaactgtat cttatgcttc agaattagga gagaatagtc agattcaaag tggttcaact
    15181 aattcatcaa ctggagagga gaaggaaagc gacaataaaa aaccagaaca gactccagag
    15241 aaagataagg caacagataa taaaaaacca gaacagaccc cagaggaaga taattcaaca
    15301 gataataaaa aaccagaaca gacttcagaa gaagataagt caacagataa taaaaagcca
    15361 gagcaaactc cagaaggaga aaagccaata gataataaaa agccagagca aactccagaa
    15421 ggagaaaagt caatagataa taaaaagcca gagcaaatac cagaagaaga taagtcaaca
    15481 gataataaaa agtcagaaca agctttagaa gatgaaaaac cactagacaa taaaaataca
    15541 gaaaaaactc cagaggaaga taatctatta gaagatgaaa atttattgaa agttttagaa
    15601 gaagaactta atgaagacta tggatttgtt gtaaaaatta ataacaatac aatagaaacg
    15661 gaatcaatga aaaaaatatc atttaattta acttatactc caacatcaaa aggaatacaa
    15721 gcaggagatt caataacttt taaggtgcca gatgtattca ataaagttaa tttagattat
    15781 acaagtgaat gctttgataa aacagaaagt aatggagagt atacacttac atttagagaa
    15841 ttacctaatg gccaaagtgt aatgcaaggt aaaataggtt tggaagcata tgttaaaaag
    15901 gttgatgaag atactaatgc aaaaatacat attgaaacca ctggaaaaat agaaagtgga
    15961 agtggagata tagatgtaga aataaaacct ggagataaaa cggatgttcc agatgctaaa
    16021 ggaacactta aaaagcttgt tgaaggaaga aaatcaacta ctgtatttat gccagttaaa
    16081 aataaagata taaattacag tatacaagta aatgaaaagc aggaagagtt aaaagatatt
    16141 attttgtatg atgagcttcc tgaaggattg acattgataa atggttctgt aagtgtagta
    16201 acaagtgatg gaaaagaagt tagtgatttt aatattgaac agagtaaaaa ttctatttca
    16261 gttaattttg gaaatataga taaatcgtat actgttaagt ataaggctag aatcagtgac
    16321 aaaaatgcaa aacatggtaa taaatataag aatgtagcta ggattgaatc tgatggtaag
    16381 aagatacaag aagatgatgc tactgtaagt atatttgata gaggagataa ttatcttctt
    16441 actaaaggac attcaggagc aacaaatatt actcaagttg gtcaagtaat aaactatcag
    16501 atttcaataa atgatgataa atcaccaata agtaatgttg ttataacaga taatatacca
    16561 gaaggtatga gattgactac ttcaggagag gatgggcatg attttagagt tgtagaagtt
    16621 ccaatgaatg ggagctggac tccttggagt aaagaaaaaa ttgccaataa tatttcatat
    16681 aaagtagaag aaaaaagaaa tgaaagtggg caagtagata aagttataac tggatttaca
    16741 attaatctca gtaaagaaga agtagaatca aagtttttta tagcttatac attaaaggta
    16801 attagtattg aagatagtta tataaataga gctgtccttg atgcaaataa tagtgaaatt
    16861 gataaaaatg atgagataaa ctttaagaaa aattctggat taataagcgc aaaaaaagaa
    16921 gttgataaaa aggttttaaa tagtagtgat aaccagattg ttaaatacaa aatcaatatg
    16981 tcaacttatg gggtttatga tgcaggacaa gtaaatcttt tggatgaagt aaacccatct
    17041 ctaaaaatat ctaatataaa atattctgat aatcttgaac ttaaaaaaga agctggagat
    17101 ggaaaaaata caataagatt agtaaataaa tacgaattta aacagataaa agaaggggaa
    17161 cctgtgcaaa gctgggttac atttgatgca gacttcacaa atgtaaaagt tggggagact
    17221 atacaaaatg tggctcagat taatggttca agtcctccgg gagtagagac tactaagcaa
    17281 ggttatgctt ttgaagctaa aaaagtggat gctttagata aaaatgtact ttcaggtgct
    17341 aagttcaatt tagaagatgc atttggaaat atagtagtta aggatttagt tagtgatgaa
    17401 gatggtgtaa ttcaatctag tgtaaaaaaa ccaggaatat attatttagt agaagtgatg
    17461 gctccaagtg gatatgaaaa actgaaggat aaaattaagg ttgaaattgg taataaagat
    17521 attggtagaa tagtagatat cggaaatata gaaaacataa gacaagaaaa tccaccagta
    17581 aatccacctg taccgccaaa tacagatgaa ccaatagtaa atccaccagt gccaccaagc
    17641 acagataaac caaggaaacc atcaaatcca tctgatacag aggatactat agttataaat
    17701 ccaccagtac caccgtcaga agatataata aatcctccaa taccagagat attgaatcca
    17761 ccagtaccac catcagaaga ggtgatagaa actcctgtta aacagattgt tccaatacca
    17821 gaagtggtaa agccatcagt gtcagaagaa aaaaataata aagctaaaga tgatactttg
    17881 gtaaatccac cagtaccacc aaaaacaggg gatagtacta caattattgg agaaatactt
    17941 ttagttatag gagctattgt aggacttgtc gtattaagaa gaaataaaaa tacaaattag
    18001 accctaacat tataaaaaat ataagttata ttaaaaaata tcttctaaaa caatatttaa
    18061 taatgaaaat attgttagaa gatatttttt tttgaagatt tatgtaaaaa aataataact
    18121 ttttagaaaa aagttccaaa gggatataga caattaaaaa gatataagct ataatataga
    18181 tataaaatta aatgaggcaa aactagggaa acctagtcac gcaaagctat aggggctaaa
    18241 gtttagtaaa ccatgccagc cagttgccga aaagagtaaa aatttctttt tgtatataca
    18301 aagatactct tgggtatctt ttttattttg aaatttatta ctcccggcct aactcaaagg
    18361 agggaataat atgagaccaa gtaaaaaatt attaatagct ataatatcaa tattcttaat
    18421 aagttcagtt ccagtaagtg cacatgcata tagtactact atacaacaaa ataaagacac
    18481 acttagtcaa atagtagttt ttccaactgg aaattacaat aaaaacgaag ctaatgctat
    18541 ggtaaataga ttagctaata tagatggaaa gtatttaaat gtattaaagc aagataactt
    18601 aaaaataaaa ttattaagtg gaaagttaac agatgaaaaa gaatatgctt atttaaaagg
    18661 tgtagttcct aagggatggg aaggtactgg aaaaacttgg gatgatgttc caggcttagg
    18721 tggaagtaca gtagctctta gaataggatt tagtaacaaa ggtaaagggc atgatgcaat
    18781 aaatttagaa ttacatgaaa cagcacatgc aatagaccat atagtattaa atgatatatc
    18841 taaatcagca caattcaaac aaatatttgc taaagaaggt cgttctttag gtaatgtaaa
    18901 ctacttaggt gtatatccag aagaattttt tgcagaagca tttgcatatt actatttaaa
    18961 tcaagataca aatagtaagt taaaatcagc ttgtccacaa acttatagct ttttgcaaaa
    19021 cctagctaaa tagagcttta ataaatttaa aataaatata aaagagatgc tattataata
    19081 gcatctcttt tatattgcgt ttaaatctaa taaattttgt cttatgtata acttgataga
    19141 ttatcaatta ctactcctta tacaaggctt caacgtgttt attatgcatt tgagtaatta
    19201 tcgtaggtat aatagaaatt attgaaccaa ttactatcaa taaaattcct ataaccatgt
    19261 ttatagggat aatttcttta agtattaata atgctagtat tggagatagt actggtttaa
    19321 agaagaatac taaagatgta agactagtag atgtagcttc cattgccata aagtaaagtg
    19381 cataaccaac acctgtaaca aatacgaata cataaatcat aatgggcata ttgtgtaagg
    19441 tatatccact aaaaataggg atatttgcaa atatatgaag tttattttta tttaatatat
    19501 ctgcgataaa attaatttta gtgagtaaaa ccaatatcaa catttccaaa ctaccaaata
    19561 taaagctaaa gcaagttaca actattccac caaattttaa ggtttttctt ttaccaaata
    19621 cagtatacat tgaaaaaata atagcagaag ataatgttaa gaatattcca ctaagagtta
    19681 gttttgtatg caatggattt attataacta taattccaag aacttctaat ataagtgaaa
    19741 ttacattgtg cctatatatt ttttccttca aaattaagta agctaaaagc attacaaaga
    19801 ctggattaca actgaacaaa acagcaacta cagatgcctt tgtgtttaga acagccagtt
    19861 gatacaatga catacttact actacacaca taaaacctaa aaatgcaaag tattttaagt
    19921 ctgattttgt aatagaaaga ctaagagctc ttaatctttt tacagcaaaa ggtagtaaga
    19981 ctaaaccgcc aacaaaaaat cttgataaag ttatttgtaa gggattaaag tcattagtga
    20041 cagtttttag actaatttcc attgaactaa aaaataaggt agtcagtatt atataaacat
    20101 aacctttttt caagtcaaac acctccttgt aatttatatt agtactatag ataagaatat
    20161 tcaagcatat tggaataata tggtatgtat aaaatatagt acataaaatt ataaactacc
    20221 ttaatataga atgtaagtat tctatactaa ggtagttaat tatttgaatt actttgattt
    20281 agttatttta aattatataa tttggctaat ttttcaaatg ctggtaaaga acttttaact
    20341 ttatcataag atacacaata tgatactcta aaatgacctg gacaaccaaa agaagaacct
    20401 ggtactaata ataaattaaa ttgttttgca tcattgcaga attttttatc atcttcaatt
    20461 ggagcttttg ggaacaaata gaatgtacca ttaggtttaa cacaagaata tcccatatcg
    20521 attaaactat tatatagaag gtcaaaattc tttttataaa tatttacatc aacttctgca
    20581 tctaaacttc ttgcaataac cctttgaaaa agagaaggag catttacaaa tccaagtatt
    20641 ctatttgcaa catttaatga agacataaca tcatcaaaat catccatttg cccattagca
    20701 acaatatatc ctatacgttc accaggtaga gataatgatt tactataaga gtaccctata
    20761 aatgaatttc tatagtattt tagtacacaa ggaactttgg caccatcata aattatttct
    20821 ctatatggct catcagaaat tagatatatg cttgtattaa attctttttg ttttgaatcc
    20881 aataattctc caagattttt taataattct tcagaatata tgacaccagt aggattattt
    20941 ggagtgttta ttattaaagc tcttgttttt ggagtaatag cattttttag agcatctaaa
    21001 tctggttcaa aagtctctat atttgtagga acttctatta attttccatc atagttttct
    21061 gtataatttt tatactcacc aaaatacggt gcaaaagcaa taacttcatc accaggattt
    21121 aaaagagttt ttaaaataat atttagacca ccagcagcac cacaagtcat aattaaattt
    21181 tctcttgtaa gatttaatcc atctttttta tttatatgtt cagcaatagc atctcttaca
    21241 tcctcatatc ctgagttgtt catatatcca tgaactaaat ttggagattc ttcatttaaa
    21301 atatcattta taactgcttt aatagtctct ggaggttcaa cacttggatt tcctatacta
    21361 aaatcaaaaa cattttcctc accataaata tcagaaagtt tctttccttc ttcaaacata
    21421 gctcttataa cagaactatt tgcaactaaa gtttgcattt tattagatat catatgtaac
    21481 actccttttt atagatttat attgcttatg tatatattac catattttta ctgaatttag
    21541 acaattttcg aaaaattact tgtttgaaaa tataaacttt tatataaaat atatttatgt
    21601 acagtgcata ttaaaaattc caatttctaa ttttatcatg attattttat ataatagttt
    21661 tttaaagaat taataaaaca tagatttaga gaataaaata ttcttgacta tattaattgt
    21721 ttaatataat atagttgata tagtcaaaca tattataatt atagaaggga aatttaagat
    21781 ggattttaaa aatttacagt atgaaagttt gtcatatatt atttgtaact tgcaaaaaaa
    21841 ctttaaactt tattgtgaaa aatgtttaaa accatataaa cttacgaatg gtctatattt
    21901 ctatttaatt tatattaata aaaataggaa ttgttctttg aatgatgttt ctacagaatt
    21961 tgaagttgat aaagctcata caacaagaac tatatctagg ttagaacaag atggatacat
    22021 agaaaaaatc caaaacccta atgatagtag agcttttcag ttgagagtaa cagataaggg
    22081 tgaggaagta ttaggggata taaaaaacat attttcaaag tgggataacc acataaaaaa
    22141 agagttctca gatacagaat ataaagaact ggtaaagaat ttacatgtgg taaaggatat
    22201 aaaaacagct gtggaggaag gataggatgt attgtaattt attaaaacca attaaaattg
    22261 gaaatttgga attaaaaaat agagtatctt ttgcacctac aagcatgggt cttaaattgg
    22321 aagaaaaaat taaaaagttt tctgatattg ctaagtcagg agtagctctg attactctag
    22381 gagatgtatc tattagacct agttttcata aggtagctat aagcctatcg gatgaagatg
    22441 gagtcattaa atacaagaaa attgtagatg aaatacacaa ttctggtgca aaggtatcag
    22501 cacaactatt ttgttcagat tatgatgtta atttaattaa agatactatg aagatgggaa
    22561 ttacttcaca tgatgagata aaaaaaataa tgaatgacgg agtaaaagat tacataacaa
    22621 atatgcccaa agaagaaatt aaaaatatta taaatttatt taaagtaaca gcattgaatg
    22681 caaaaaaagc aggttttgac atgattcaaa ttcatggtga taggcttgta ggaagttttt
    22741 catcaagtat atttaataat agaaatgatg aatatggtgg aacgtgtgat aatagaagta
    22801 gatttgcaag cgaaataatt agtagtatcc gagatgaagt taaggacatt ccaatagact
    22861 ataaatttgc aataagacaa gaaaatcctc attatggaaa tgcaggagtt ttactttcgg
    22921 aagttgagta ttttgttaaa aaatttgaat ccctaggtgt aaacagtttt catgtaacac
    22981 ttgcaaatca ttcaaaatta gaagatacta taccaaccaa taatcatcct tactttaaag
    23041 atgaaggctg ttttttatat ttagctgatg aagtaaagaa acacacaaat cttccagtat
    23101 gtggggttgg aaaattgtct agcccagatt ttatagaaag tattatttca aataatagag
    23161 tagatatggt ttctatgtca agacaattac tagcagatag taattggtta cagaaagtaa
    23221 aagatggaag agtagatgag attaaaaagt gctgttattg taataagaag tgtgcagatg
    23281 ctttacaaac acgctctcag tttggatgta tactagatta gaatatcttt ataaaaataa
    23341 ataatttgtt aaggttaata tatttttggt gatttagata agtatttaga taaagaggag
    23401 gagacatcat gaaaagttta gttttatatt caagtttaac tggaaatact aaaaagattg
    23461 cttatgctat atatgatgaa attcaagaag aaaaagacat taaagatgta aatgaattag
    23521 ttgattaaaa aatagctacg cacacttgtg actttagtca tgagttagta gctaaaatag
    23581 tcagcatata gggaaaccta tatgtagaga taggatagaa cctatccaat actacttgaa
    23641 ttgctggaaa cccctaaagc taatcaaact aaaacataag g
//
LOCUS       DAELSY010000056        23361 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.056, whole genome shotgun sequence.
ACCESSION   DAELSY010000056 DAELSY010000000
VERSION     DAELSY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.056"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(85..1041)
                     /locus_tag="KPG09_001880"
     CDS             complement(85..1041)
                     /locus_tag="KPG09_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBG0966490.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFIGIIIFKIKKLL"
     gene            complement(1380..2078)
                     /locus_tag="KPG09_001881"
     CDS             complement(1380..2078)
                     /locus_tag="KPG09_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0966491.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            complement(2112..4814)
                     /locus_tag="KPG09_001882"
     CDS             complement(2112..4814)
                     /locus_tag="KPG09_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBG0966492.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEVITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSSKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            complement(4992..6809)
                     /gene="ftsH"
                     /locus_tag="KPG09_001883"
     CDS             complement(4992..6809)
                     /gene="ftsH"
                     /locus_tag="KPG09_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG0966493.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            complement(6840..7283)
                     /locus_tag="KPG09_001884"
     CDS             complement(6840..7283)
                     /locus_tag="KPG09_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0966494.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            complement(7774..8664)
                     /gene="metA"
                     /locus_tag="KPG09_001885"
     CDS             complement(7774..8664)
                     /gene="metA"
                     /locus_tag="KPG09_001885"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBG0966495.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(8694..9959)
                     /locus_tag="KPG09_001886"
     CDS             complement(8694..9959)
                     /locus_tag="KPG09_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBG0966496.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(10069..10171)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(10225..12882)
                     /locus_tag="KPG09_001887"
     CDS             complement(10225..12882)
                     /locus_tag="KPG09_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG0966497.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNFSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKRKK"
     gene            complement(13010..13753)
                     /gene="yaaA"
                     /locus_tag="KPG09_001888"
     CDS             complement(13010..13753)
                     /gene="yaaA"
                     /locus_tag="KPG09_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBG0966498.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIIN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            complement(13763..14227)
                     /gene="bcp"
                     /locus_tag="KPG09_001889"
     CDS             complement(13763..14227)
                     /gene="bcp"
                     /locus_tag="KPG09_001889"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBG0966499.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            complement(14579..16276)
                     /gene="ade"
                     /locus_tag="KPG09_001890"
     CDS             complement(14579..16276)
                     /gene="ade"
                     /locus_tag="KPG09_001890"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBG0966500.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGASAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIASLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            complement(16582..17736)
                     /locus_tag="KPG09_001891"
     CDS             complement(16582..17736)
                     /locus_tag="KPG09_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBG0966501.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKFYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            complement(17981..18820)
                     /locus_tag="KPG09_001892"
     CDS             complement(17981..18820)
                     /locus_tag="KPG09_001892"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBG0966502.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFNNSKKYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            complement(18833..19429)
                     /locus_tag="KPG09_001893"
     CDS             complement(18833..19429)
                     /locus_tag="KPG09_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG0966503.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            complement(19480..20130)
                     /locus_tag="KPG09_001894"
     CDS             complement(19480..20130)
                     /locus_tag="KPG09_001894"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBG0966504.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGVDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKSSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            complement(20176..21429)
                     /locus_tag="KPG09_001895"
     CDS             complement(20176..21429)
                     /locus_tag="KPG09_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0966505.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEKENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLREIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            complement(21453..22094)
                     /locus_tag="KPG09_001896"
     CDS             complement(21453..22094)
                     /locus_tag="KPG09_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG0966506.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            complement(22296..22709)
                     /locus_tag="KPG09_001897"
     CDS             complement(22296..22709)
                     /locus_tag="KPG09_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBG0966507.1"
                     /translation="MIELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNLYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            complement(22860..23201)
                     /locus_tag="KPG09_001898"
     CDS             complement(22860..23201)
                     /locus_tag="KPG09_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBG0966508.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
BASE COUNT         7369 a         4154 c         2474 g         9364 t
ORIGIN      
        1 tcaatttaaa atattttttt atttacttaa atcagcttct caattgatgt atttttataa
       61 aatcaattaa acatcatctg taatttacaa taattttttt attttaaata ttataattcc
      121 aataaaaatt atacatagca taaaaaataa agttgctgga tatccataaa tccagtttaa
      181 ttctggcata tatctaaagt tcattccata tataccagtt ataattgtaa gtggtgctga
      241 tatggctgtt aacaaggtta attttgtaat taagttattt gttttctcac ctactctgga
      301 agaataaata tctaataatt tatctgctaa ctctcgtgtt gacaatgcga aattgtataa
      361 tttgtcaatt ccaaaatcta taccttgaaa attttctaaa ttatattttt ttacatttga
      421 atattttaaa tatcttatat tttccttaac tattctatcc ccaatataca aaagaggtct
      481 agtattctta actataattc ttgttaatcc tcttacatca cttattctag atacatgatt
      541 cttattaatg ttgtccatca tttcatcttc tatttgaaga attaactctt caactttttc
      601 caaagactca aaaccatttt ttataacttc tctaagaatc aagtagttta tcttaaatag
      661 attaataact ggattttttt caagttgaga gttcttcaaa ataatatttt ttacaaattc
      721 aaataaaaag tgttcttcat tcactacaac caatataaaa ttatctgata aatacatatt
      781 tacttcttct ataacagctt ctccatctct tagttcaaat gtatttaggc ttaaaaaatc
      841 gtaattatca aataaatcaa gtttggttat ttcatcaaat ttaagacaat cattaaaagt
      901 tatttcatct atatccaaag tttccttaag caattttaaa ttttttggag cacttaatat
      961 aagataagaa ttttctgcta cataaaattt atctctaaaa tcttcaatag tctctcttgt
     1021 atttaaattc agtatataca tacatctact cctaaatttc aatttacatt ctaaaaattt
     1081 tttataatcc ttactgtaat tattttacac tattttaaca attattattt aatatttaca
     1141 ttaaatttat tataaggtaa gtgttaaata catttattat ttaaccaaag tttttattac
     1201 agattacact atatacttat tttaaaacat atttcatatt attgatacta aaaagctaaa
     1261 aaataaaatg catgtgctaa actgacaaca agcacataca cttcattctt attttaacta
     1321 aactatataa ttttaatata tattaataag cacataatct aataattaat atattacaac
     1381 tattcatcat ttaatctgta tcctacacca acttctgtaa ttatatatct tgggtttgca
     1441 ggttgttttt ctatttttcg tcttaatgtt gccataaaga cccttaaaga cttagtctca
     1501 ttccctataa cactacccca tatttctttt attataaaat tatgagtaag cacttttcca
     1561 tgatgctttg caagtaatga aagaatatta tattcaatag gtgtcaaatg gacttcaaca
     1621 tcatcaacaa taacttttct tttatcaaaa tcaatcaata atttatctac ttcaaacgta
     1681 gatttaacat catctttttg ttgagcttct acttgtgaat gtctaaatgc gactcttact
     1741 cttgctaata actctactat ggagaatggt tttgttaagt aatcatctgc ccctgcatca
     1801 aatacttcta ccttatctct atcttgctct ctagctgaaa ctacaattat tggtatattt
     1861 gaaaattgtc tgatagcttt tattacctct attccatcca tatcttctaa gcctaaatct
     1921 aaaagaacaa catctggaga atatgagaca gcaatagata taccttcttt tccagttata
     1981 gcctccttga catcataatc ctgtgttaaa agtgcagtag atataaattt tcttattgtc
     2041 ttatcatcct ctactaacaa tataagtttt ttactcattt cttcctccaa aactaatttt
     2101 aaaatttaaa actattcatt ttccttaggc aaactaaact taaaagttga acccttatct
     2161 ttattgtttt ctaccataat ttcaccttta tgagcttgaa ttatagattt acaaattgat
     2221 aatccaagtc caacaccctt tctggaatct ttagctcctt cttctccagt aaaaaatctg
     2281 tcaaagatat gctttttaag ttcctttgat ataccaggac cattatcaga tacttcaaac
     2341 caaacataat catctttctc gtatacattt atctctatct tacaatcttc ctttgaatat
     2401 ttcaaagaat tatctattaa attaattaaa acttgttcta ttaattttgc atccatagga
     2461 acaaataata tctgttctgg tatattaata tctataagtg cattttcaat tctttttttt
     2521 attttttgta acgcttctga tataatttcc tcaaccagtt cagaatgttt tttaacttcc
     2581 aatttccctt catctatctt ggtcatacta agtaaatttt caacaagtct tattaaccac
     2641 tgagtatctt catatacacc actaagtaat tcatctatta tatcttgacc tatagtaccc
     2701 ttgtttttta taattgtact tactgcacca gatattcctg ctagtggagt tctaaggtca
     2761 tgcgaaactg ctcttaaaag attacttctt aatctttccc tttcaatctc aagattggta
     2821 ttttctttag cttctgacaa gatttctcta tcaagcgcta ttgccatttg agcaattatt
     2881 gtttcaatta agcttatatc ttctgtatcc aattttgaat caatacatga tattcctact
     2941 actcctaaag ttaaattcat tcctacaatt ggtatatagt atccctttga accaggaagt
     3001 gtatccgtgt catttcctgc cactgttgaa ttggataaaa cccaacttgc aacagctttt
     3061 tcatcttcac tatccaaatc atttttatcc ttattattag tatttttttt gtaaagtttt
     3121 gatttattat gcttatctac atttaaataa caaattacat ctctacttaa gctaagagac
     3181 aaaagttcta tacctttgtt tactatatca tctttacttg ataatcttaa aaatccctta
     3241 attatttgat atatcatttt tgtattttct tctcttttag cagcagtttc tgcttcatgc
     3301 tgtatcttag acgtaagagt gctagttatt atacctacta ttgagaatac aaccagtgtt
     3361 actatatagt ttgaatctgc tatagataat gtatataatg gtgctgtaaa aaaataattt
     3421 aaaagaacta tattaaatac agaacttatt atacctgtac tatacccttt agtccacata
     3481 gaaactaaaa ctacacccaa catatatatt aacaatatat tttctcttat aaatcccatg
     3541 ctttgtactg catatgctaa aatacttatc atcaaagtaa taaatataag ttttataaaa
     3601 tcaactttgc taattttaaa tttagatttt attgatgatt tatccttttt aggtttgtat
     3661 ttaacatctg aggcaaattt atatggaatt atatgtatat ctatataatc aacttcatca
     3721 attaatttgt caactatatc ttttttaaat cttttactaa atcttttatt acttgaatga
     3781 tttctaccta ttacaatttt tgttacattt ctcagttttg caattctaag tatttgctct
     3841 attatatttt caccatgtaa aacaattatt tctccaccta atttcttagc taattttagg
     3901 ttattttgta gttgtttttg ttcatcaaat ggaagtcttg ctgtgtttgg agtttcaaca
     3961 tataaagcta tccattttgc aaatgtactg tctgatattc tggatgcagt tctaataact
     4021 tttgctgaag atggagatgg acttatacat gcaagaagtg tatctgatgt aggtattaca
     4081 gttacatcac cctttgatag tctggtcatt tgaacttctt tattaactct ctctgctgtt
     4141 tttctaagtg cgatttctct aagtgcaacc aggttatctt ttataaaaaa attatttttt
     4201 gctctagtag cttgttcttt cttatatatc ttaccttcat tgaacctttc aagaagtaca
     4261 tcaggttcaa catctataag ttctaactgt gtatttgtat ccaaaaactt atctggtata
     4321 gtctctttaa ctgatacgtt tgtaattacc tcaacaatgt catttagact ttctaaatgt
     4381 tgtacattta cagtagtgta gacatttatg cctgctaaca gcaattcttc tatatcttgc
     4441 catctttttg tatgccttaa cccagcaaca ttagaatggg caaattcatc tactaatata
     4501 acttctggtt ttcttgctaa agcagcatct aaatcaaatt cttttagttt tatagattta
     4561 tattctatct cctttacagg aagaatttcc aagccatcaa gtagagccaa tgtttctggt
     4621 ctagtgtgtg gttcaatata tccaactact acatctacac ccactttttt taaattttgt
     4681 gcagattcca acatagagta ggtttttcct actccagctg cataaccaaa gaatattttt
     4741 aattgacctc tacttagttt ttcttcttca tttttaattt tttctaatag aatatcagga
     4801 ttaggtctat ccattaaaaa acctccttac accattcatt actattataa tatcaattcc
     4861 gatataaaat tgtagttaag atgtattttt aatgattaag attatgtaaa gatttaaata
     4921 tgaatttaaa aaatttagtt gtaaaataaa aatgcctaac aataggcatt tttatcttgt
     4981 gtataatcat tttagtttaa gttaagtatt tccataaact catcacctgt aatagtttct
     5041 ttttctaata aataattagc aagttcatgc aatttatcaa tattattttc taagatttca
     5101 attgcatttt tgtgacaagc ctttactatt ttaaatacct cttcatctat tttagcagca
     5161 gtgtcggaag aacaagcaag agaagtatct cctcctaaat attgattgct tacagtttca
     5221 agagcgacca tatcaaactc ttcactcata ccaaggcgag taaccatagc tcttgcaatt
     5281 ttagtagctt gttctatatc atttgatgct cctgatgtac aagtgttaaa gataacctct
     5341 tcagcagcac gaccacctgt aaaagtagtt attttatcaa ttgcttcttc tttagacata
     5401 agtacactct catcttcagc aacttgcata gtatatccca atgctcctga agttctaggt
     5461 attatagtaa tcttatggac aggtgcagaa tgtttttgca tagcagcaac aagtgcatga
     5521 cctatttcat gatatgctat aattttcttt tccttatctg aaattacagc tcctttacgt
     5581 tgatacccag caattacagt ttcaacagat tcatcaaaat cctgttgttc tactatatct
     5641 ctgccagact taactgctaa aagtgctgct tcgtttacaa tatttgcaag ttctgcacct
     5701 gatgctcctg atgtagacct tgcaattgca ttataatcta tatctgagct tacatttaca
     5761 ttttgagcat gtacctttaa tatagcttca cgtcctccaa ggtctggaag ttcaactggt
     5821 atacgtctat caaatcttcc tggacgaagt aatgctttgt caagactgtc aggtctattt
     5881 gtagcagcaa gtattactac tccaattcca ccatcaaaac catccatctc acttaacaac
     5941 tgatttaaag tttgttcacg ttcatcattt ccacctaatc cacttccatt atcacgcttt
     6001 ttaccaatag tatcaatctc atctataaac actatacatg gagctttttc ttttgcttgt
     6061 ttaaataaat cacgaatcct agaagctccc attcctacaa acatctcaac aaactcagaa
     6121 ccagatattg agaaaaatgg tacatttgct tcacctgcta cagcttttgc aaggagtgtt
     6181 tttcctgtac ctggaggtcc tactaaaagt gctcctttag gcatttgtgc tcctatcttt
     6241 ttatactttt ctggattgtg taagaaatct actatttcag ttaaagcttc ttttgcctca
     6301 tcttgaccag ctacatcatt aaatcttttt ccactttgag cagatacata cattttggcg
     6361 ttgctttttc caaattgcat agcattacca cccattcgct tttgcataga cttcatcatt
     6421 aagttaccaa atagcataag aatacctata ggtattatcc atgtaagtaa aatacttaaa
     6481 agaggtgaat tttccttagg aataacctta gtaaattcta cttttgcatt ttttagtcta
     6541 tctaccaatt ctgggtcatc cattctacca gttatgtata tattcttatc cttgctacta
     6601 gatggattta ttgctattct attattttct atctcaacag atttaacctt tcctgtatcc
     6661 aacattgtga gaaactttcc atagtcaatc tcaaccattt ttttatccat taaacttgga
     6721 aatattaaag catttaaaaa cattactact attattataa ctgtataata atagagtgct
     6781 gacctttttt ggggtttatg ttcatccata attttattct cctttaaatt atttttatgt
     6841 tatttgcaat caatgtaagt taaaagttta tttaatttct ctaaaccttc tattatatct
     6901 tccatatctt gcaatttttc actggtcata ttttcaaaat aactatcaaa aatactactt
     6961 gaaatatgtt cttgtatatc caatgattta tttgtaactt ttacttttac aattcgttgg
     7021 tcttctatat ctcttatgcg atttaaaaag ccattttttt caagcctttg acagattact
     7081 gatacattac tatttgtcat gtttaagttc ttccctaaat cgcttatttt tttctctcca
     7141 tgttcacata actcagataa aatcaaagct tgcactgctg taataccaag tggctgataa
     7201 aaatcactta ataaagttgt aaatttcata tttatatctc tagtcaactc tagtagctct
     7261 ttcttaaaac tcatctcatc catacaaacg ctccctacga aaatacttta tttcaaattt
     7321 aggctgaagt gcatactgta aataaatact taatactata atgtttaatg tattaaatat
     7381 tatagtatta agtatttatt gtcaattaca atattgtaac ttcaattgga attttgttat
     7441 cttattgata tttaatttta tacaggatat ttttaataaa atttaataaa aaatcgatat
     7501 atatatttta cactgatttc tcccttttta atctctttta aaagttaaaa tacaaattta
     7561 ttgatactat aaaggtttag tctcccatta tcgcaccata tataaaatta aaaccacctc
     7621 tatctacata cacctaaagt gattttctta aaatataaaa ctactattat aaggttaaat
     7681 ttaaaaaaaa taaggtataa agtatcatta agattaactt tatacctata aatttctata
     7741 tttttttagt taacatttat atactcttaa aatttattgt atcaaatagt tttttatcca
     7801 atttctaaac aacaattctg aatgtttttt ccattttaca gttggctctt ctgaagggtc
     7861 atcattaata tagtaatttt gaggtataga tattttatct cctttattta tatccctctt
     7921 atattcttta tcaagagtgt atttgtcata ctcgctatga cccattacga acacatctct
     7981 tgaatttgta attatatagg gaccagcttc tttagaatga ctcacaatgc tcaactcttt
     8041 attctctttc aaagcttcaa tatttacagt agtatgtctt gaatgtggag caaaaaattc
     8101 attttcaaat ccatctacta ttttactatc tttatctacc ttatgcttaa atactccaaa
     8161 acatttttga cttagtggaa gtttttttac attgtagtat ttgtaaagac ttgcttgagc
     8221 tgcccaacat atatataatg tagatttagt ttttctctta gaataatcca taatttcttc
     8281 tagttcattc caataatcaa cttcttcaaa ctccatttgt tcaactggag ctcctgttat
     8341 tataaagcca tcaaaatact ctcctttggc ttcatcaaat gttttataaa attctttaac
     8401 atattcatta caacttcttt tactttctct tgttgaaatt ttaataaatt caacagacac
     8461 gttgaatcct gttttatcta tccttcttag taaatctaat tctgtatcta tctttatagg
     8521 cattagattt agtatagcta ttttagtgtc tatgttttct tcatacttta attccttttt
     8581 aaaatcttct ttataaatta catctattcc ttcatctaat aatttattaa taacaggtaa
     8641 tccctttggt agtattaaag ccatatcttt tcacttctct ttctcttaga atattagaaa
     8701 tttaaagctt gatttaaatc agctattaaa tcctctacat tttctattcc aacagataat
     8761 cttattagtg aaggcaatac tccagatgca attaattgtt cttctgttaa ttgtctatga
     8821 gtagttgaag ctggatgtat aacacaagtt cttacatctg aaacatgtgt aaccaaagat
     8881 gctatctgta atttttccac aaattttttg gcattttcta acccacctct tactccaaat
     8941 gataaaacac cactacatcc tcttgataaa tactttttgg cattctcata atacttgtta
     9001 tcttcaagtc ctgggtaatt aatccaatct acattttcat gtttttctaa aaatctagct
     9061 attttaagtg cattttcact atgtctctcc attctaagat gtagtgtttc aacatttaaa
     9121 ttagtaagat acgcattgaa tgggcttaaa caatttccat agtctctaag caactgaact
     9181 cttgctttag ttgcatatgc ggcatttcca aatttttgag tatagcttat tccatgatat
     9241 gttgggtctg gctcaactaa ttctggaaat tttccattat cccagttaaa gtttccacca
     9301 tccactataa ttcctccaac acttgaagca tgcccatcta aatattttgt ggtagaatga
     9361 gtaactatat ttgctccata ctttaaaggg ttacaaagca ctggagatgc taatgaatta
     9421 tcaacaataa atggaacatt aattctctta gctacatttg ctattttttc aaaatctatg
     9481 acttcaagag tcggatttgc aagtgtttct gcaaatacaa ccttagtatt ttcctttgca
     9541 agttctacta tctcatcttc acttgaatct aagtcaaacg atattaaatc tataccaaat
     9601 tttttaagac taggtcctag taaattgaat gttcctccat atacttttga agaacaaagt
     9661 atactatctc ctgatttaca tatatttaaa actgccaaca tatttgcaga ttgccctgat
     9721 gatacagcta cagaagatac tccaccctct agtaaactta ttttttcttc aaaagcttga
     9781 atagtaggat tgcttattct tgaatatata tgtccatcaa cttttaaatc aaataattca
     9841 gcaagttggt ctatactgct atatttaaaa gttgtacttt gatataaagg taatactctt
     9901 ggttctccat tacctggttt ataatttcct tgcacacata ttgtttcttt attatacatt
     9961 cttgtatccc ccttagattt tagtaaaaaa aattaaacta tttatcagat gagagttaaa
    10021 ataaataaag cctgagtata taactcaggc tctgtataag cttaattata ctcatctctc
    10081 agctttcgcc gcaggattta gcaccaatgt atataaatat actggttgcc gggtttcata
    10141 gggccagtcc ctccacctct ctggataaga tttatttaat ttttctatgt tttatatctt
    10201 atatattttt cctaattttg tcaactattt ttttcttttt tttcttttaa taattttccc
    10261 aacctctact tgaattacag acattatggc taaaacaatc gttataagcc actctaagtt
    10321 atttaaaact gttaagttaa aagcatttct aaaaacaggt acaaacagta ttactgctat
    10381 taaaaatgca gaagttaaaa atgagattaa aagaaatgga tttttaccat tatttctatt
    10441 aaacattgaa tctgtatttg aacgctgact aaatgctcgt aacatctgtg aaaatgctaa
    10501 tacacaaaat gccattgttt gacctacaga atgattttct atattaagac caattttata
    10561 tgctaaaata gtcattattg ccacaaatat accttgggta acaactcttt gtataagatt
    10621 tttttcaaac aaagtggttg atttcactgg ctggtgcttc attatatttc tgcttggtgg
    10681 gtcaactcca agagcaaggg ctggaagcgt agcagttgca aggtttaccc atagaatatg
    10741 aacagcaaga agcggagtat cccaatttaa taatattgct acaaacaacg taagtatctc
    10801 tgctatatta ccaacaagca aaaattgtat aactttttgt atgtttctat atacacgtct
    10861 accttctttt attgcataag caattgttgt aaaactatcg tccattaaaa tcatgtcaga
    10921 agaatctttt gcaacatctg taccacttat acccatggct ataccaatat cagcagactt
    10981 taaggcagga gagtcattta caccatcccc agtcattgct ggtacttctc caattctttt
    11041 tagagattgt ataattctaa gtttatcaaa cggggacaca cgagcaaata ctgtagtatt
    11101 tttaacagct tcatctagtt catcatcact aaaattatca agttcatcac cagatatcac
    11161 agtattttct tcactccata ttcctaactc atgagcgatt gttgtggcag taactttatg
    11221 gtcacctgtt atcattatgg ttcttatacc tgcttcttta caagtactta ctgcatcaat
    11281 tacctcagtt cttggtgggt caatcatacc aaccatacct ataaatgtca aatcatattc
    11341 tatattttca ctatcttctt ttggtataga tgaaatctca cgcttagcaa aaccaagtac
    11401 acgcaatgca tctttagaca tgttaagaca taattcgtgt atattctttt tatccacttc
    11461 ggtaatgttt ctttcacctt ttgaagttaa tattttagta cataggtcga gaatttcgtc
    11521 tactgctcct ttagtataag caataatctt ttcatctata ttatgaacag ttgtcataca
    11581 ttttctatct gaatcaaaag gctgttcaaa tacacgtgga tattcttctt caagagcttc
    11641 ttgatctatt ccaaattttt tggccataaa aattaatgcg ccctctgttg ggtcacctaa
    11701 tatttcatct gttttatctg gattaataca tgcatcatta caaagtgcac cagcataaac
    11761 aatctcttta tacactaatg gatgtttttc agatgcagta tctattgatg tcacttttcc
    11821 agattcaaaa tctccattga cagcaatatg tgtaacagtc attttattta aggttaatgt
    11881 accagtttta tcacaacata ttactgtagc tcctcctaat gtttcaacag caggtaattt
    11941 tctaattaaa gcatttcttt tagccatacg ctgtacgcct aaagccatta caatagtagc
    12001 tgttgcaggt aatccctctg gaataattga tatggcaagg gaaattgcaa tcataaacat
    12061 tggcaagaga ggtctctgat aaaatgcacc tattgcaaat ataacaacgc atacaataat
    12121 acctatgaca cttaatgtct ttccaacagt attaagttta cgttttatag gagtatcaaa
    12181 atcatcttgt gaatctaaaa gatgtgctat gttacccact tctgtatcca taccagttgc
    12241 tactacaaca cctacacctc taccatatgt gactattgaa gatgtatacg ccatattgct
    12301 acggtctcct aatacacatt ctttaggtaa gattgtatct gcatcttttt ctgatggaac
    12361 agattctcct gttaaagaag cctcttgaat ttttaaactt gatgtttcta ttaaacgaag
    12421 gtctgctgga accatagctc catcttctaa aaaaacaata tctcctacta caagattact
    12481 tgctggaata acattttctt caccatcacg cataacacga gctgtaggtg agttcatatt
    12541 ttttaatgct tctaatgatg attctgcttt tttctcttga acaattccaa taactgcatt
    12601 tactgttaca attattagta ttacaaaagc ttctgtaaat tcgccaaata tcaaagataa
    12661 tattgctgct cctattagta tcataaccat agcatctgta atttgactcc aaagcatttg
    12721 taaagttgtt ctaggtggtt tttttctaag ttcatttaag ccattctctt taagccgttt
    12781 ttccgcctcg gtatcggtga gcccctcctc tgatgaatct agactttcta aaatttcatg
    12841 cgttgctctt gaatgccaag gcatctgttt gtgattttcc attttggaag accactcctt
    12901 tcttttttta atttaagaat aattatattt taaaatcaag ttcaattcaa tacacactaa
    12961 gtatatttta cctaaattta acaatgccat ttaatattta acaaaacttc tactctttta
    13021 caaatatgta ttcactattg ttagataagt tttctttata cttataacct tctaaattaa
    13081 actttttcag ttcttcaata tctcttatcc tattttttac aatgtatttt gtcatcatac
    13141 ctctagcttt ctttgcttgt gtagattcca ctttcaatat gccattttta tttactttaa
    13201 aagtacatgt aatatatgta tcactatctt taataaattt ttctatggac ttgctgtatt
    13261 catttgatga aagattaatg atagctgttt tttctttgat tagttcttta tatatacgat
    13321 ttccccaata ttcgtataaa ttcttaaact cacctactcg taattttgtc tgcatttcta
    13381 atctatattc atatatacta tcatatggtt ttacaacacc atacaatcca cttatgattc
    13441 gaatacagct atttgcaaac tcttcatctt tccttgtaaa attttctgct tctatatttt
    13501 tatattgtat tccatcataa gttaagattg cagggtttcc taatctatca aatttaatgc
    13561 tttcaaatct atttttattt aatacagcta atttttcatt aattttcatt ttatttttaa
    13621 aatcttctat accaaatgtc tttatatttt ccactatttc ttttgtgtta tctataaaac
    13681 aaggcaaact caaatctcta tcaaatactt ctatattctt catattttta gctggtgata
    13741 acattataat cataaattct tattactcct gtttttccaa atattcaaga atctcttgtg
    13801 catttgtgtc tggttttgct ttttcaaaga ctttttcaat aattccattt tcatctataa
    13861 tatatgtcgc acgtacaact cccaatgcag tttttccata catttttttc tctttccata
    13921 catcaaaagc ttttatagca actaagtcag ggtcagaaag taaaataaaa ggaagttcat
    13981 gcttttctgc aaatttttga tgtgatttta tactatcttt acttatccca ataacttgta
    14041 tatcttcctt tttaaatcca tcatatgcat ttctaaaagc acaagcttga cgtgtacatc
    14101 ctggtgtatt atctttagga taaaaatata ctactacttt tttgccttta aaatctgaca
    14161 tacttacctt atttccatct ttatcttcta gtgtaaattc tggtgctttt gttcctatac
    14221 tcaacataaa tatctctcct tttgttttta ttaataattt tatactatgc ttatattatt
    14281 accctataaa tctatatata aataaaatat tttatttttt atgtataaag tttcataaat
    14341 atataatttt catgtattta tattctaaaa tagactgaaa atagttttat taagatatct
    14401 atgcttaaaa caaggttaaa tgatagatta attaaaataa ggatatctaa aaataaaatt
    14461 tttagttcat tagatgctct tttttgtatg aaatcaaata tattttcatc atttcaacaa
    14521 taaaaagaga cttatcccta ttttgagaaa gcctcattta ttattacttt tatattatct
    14581 atataaattt aaattcctca acatcaaata gacctaaatc tgtaagtctt atttgtggaa
    14641 caactggaag tgccataaaa gaaagtgtaa taaaagggtc tacatactct ggaactccca
    14701 tttttcttgc tatctccaac atattatcag taatttcttg tacttcttca gctgtaaaag
    14761 tactaattaa tccagctact tgaagactta ggtgtgcaag aacttttcca tttgatacaa
    14821 ttacataacc accttgtatt tcctttaagt aatttatagc tactagaata tcatcatcat
    14881 tatcccctgc aactataata ttatgagaat catgggccac agtagtagca attgctccat
    14941 tttttatgcc aaatcctttt agtgaagcaa ttgctacatt tccagtcatt ctatgtctct
    15001 caacaacaca aagttttgaa tactctttat ttggaacaaa atatccatct gcacatggta
    15061 aagattcata taccttattt gtcaaaattt gataaggtac aatctccatt acataatttt
    15121 tttcacttac ttttaactgt attttttctt tattaatata cttaaacttg actgtattaa
    15181 gcaattcttt atctttaatc tcataattgt aattagaaaa catctcttcg tttaccaaat
    15241 taccatcttt atatacacta tccacttcca ttttgtctaa atcattcaat acaacaatat
    15301 ctgccttata acccgcacct attgctccaa ttttccttaa cccatatgct tttgcagaat
    15361 tataagttgc aacttttata gccctaacag gttccatacc taaatcaatc gcacatttta
    15421 tattccatct tatgtgacct tgtttttcta tatcatctaa gtgcttatca tctgtgcaaa
    15481 acataaattc ttcaattggc aagttatgct ttagcatacc acttactata gactttaaat
    15541 tttttgctgc gcttccttct cttattaaaa tttttaatcc tgctctcaat ttttcatata
    15601 cttcatcaaa agttatacat tcatggtcat tttctattcc tgcacataca tatgtctgaa
    15661 gtgatttacc atttatatta ggtgcatgtc catctacaac tttattttta aaaagctcta
    15721 gtttatccaa gatttcattt tcagaattta taacatcatt aaaacacatt acctctccta
    15781 aacctaaaat tctaggatta tttacataac tctccatgtc ttttgcacta aatttaccaa
    15841 ctccattagt ttcaaaactt gttgcaggta cggatgaagg caccataata tatacattaa
    15901 gtggtatatc ttttgtcgat tctaataaaa aatctattgc actagctcct tttacattga
    15961 ccaactcatg tgggtcggct atgatagttg tcgtacctga ttttaataat tttttcgata
    16021 attcaattgg cattaccatt gtagattcaa tatgcatatg tgcatcaata aagccaggtg
    16081 atatatattt attgctacaa tcaatttcta cattgccctt gtaatcgcca atgccaacga
    16141 tagtgtctcc tactattgct acatctcctg ttattaactc ttctgtaaat acatttatga
    16201 ttgtcccatt ttttagtaca atgtcagctt tttctcttcc acttgaaaca tcacactgtc
    16261 tttttaaatt acccattttt acaactcctc ctaagttttt tatatcaata tactggctgt
    16321 attacgtatt atagtttaat ttattaatat atattatata tgagttttga tagaaagtat
    16381 cacttgaaat ttgttccata attcctttaa ttaaatagag agaacatgag gaataatatt
    16441 atctcccatg ttctcattaa tatttaagca ataatttaat tcatattgaa ttttttattt
    16501 ttatatttaa tcttcactat tcttataaat aaatttatgt gctttattat ttttttaata
    16561 cttctttatt cgtaattata tttaacctaa aaatgcataa actatcatac ctacaagtat
    16621 tgctggtata gtagtaataa tcatagccca taaagaagat tttttatcca ttgctaaaag
    16681 tgggaaaagt gcatctccat cttgagaaat agagttggct atcactgcac caagtggtat
    16741 agttcccttc aaataaaatg acataagcac aatttgtatc ccacaacctg gaataagtcc
    16801 aagacctgca cctataaata cacttattac acctgttgat aatactagtt taactaagta
    16861 ttcatctcca cctaaaatta atattatcaa atcgtaaata agatatgcaa taaaaatcca
    16921 tgtaattaca aatgatattt ctccaacaga atggataagc atttctcgca aagaaatctt
    16981 tttattttct tcctcttttt gattagaatt tttaaatacc tttttaaaac accacatata
    17041 aattattgaa aataatattc caatcactgc tattacttct tccaatgaat gtaacttctc
    17101 aattatagga agattaagtc cagagtgagc aagtgacata aatataaaac cgattactat
    17161 aattcctata taaaatttat atccctttcc atgtgttatt ataaatgcaa gtctatttgt
    17221 atttccatga gatttagcta gagtgtctaa aattatttca tcatgattat gttgttcttt
    17281 attttcttta gggttatttt tacttatatc atatttatct gattttctac ggttttttaa
    17341 ccctaatttt tcttctagtt taaagtaatc cactaattgt cccgttatta ttcctgtaat
    17401 tgtactaact atagtgacaa aaaaatacat tttaatattt gatgaaataa gtacaaatgc
    17461 tgcatctccc atactagcta ttaaacttgc aattattgct ccaaagctta attttccatt
    17521 tatatacaat ggcattattg ccagacttcc gccacaacct ggtatagctc caattaaagc
    17581 acctattaag ggttgaaatt ttctgttttt tgatatgata ttagtaaaat ttccactagt
    17641 tttataatta atatatccaa ataacaaaat aaaaaatcca atcatagaac ctacatgtaa
    17701 aaacgcctct tcagaagcgg atatcaacat ttccattaat ttacctccta gactaatttt
    17761 gcaacaatta tattatattc tattttctat tgataatcaa tatcaatatt tcttttgaat
    17821 ttttaatatt aaaaagtgcg catcttatac tcttagtttt tcatcttaag atacacactt
    17881 tttgatatta ggttatctat ttaccacatg taatatgaaa tcaaaggtgg atattatgat
    17941 aaattataac aatgtccacc tatttttatt acctatcaaa ttataaattt tcaaggaatg
    18001 ttacaacttc ttctattatc caattaggtg tagatgcccc tgctgtaata cccaaatttt
    18061 tatatttttt aagcgtattt acatccaatt catctttagt ctctattgca aaagttggta
    18121 ctaagtcttc acaaaccttt actaactttt gagtatttga actgtgttta cctcctataa
    18181 ctatcataca atcaacttct ttagctaact ctttagctga ttcttgtctt gtttttgtag
    18241 ctgaacaaat tgtatcatta aatactattt cttctaattt atctgatagt ttattaacta
    18301 tacttatata caactctgga tttattgtag tctgtgcaac aagacaatat tttttggagt
    18361 tattaaattc catattttcc atttgttcta aggtctttat tattatagct gaattttcac
    18421 accatccatt tatacctata acttctggat gcttcatatc tccaataact attatttcat
    18481 agcctttatt atgatattca cttactactg tatgtatttt ctttacaaat ggacatgtag
    18541 tatccacaat ttttaatttt ttatctttgg cttccttata tatattttca ggaactccat
    18601 gagaccttat tatcatattt tcatgatttg gtattgtatc aatttcattc acagtcttaa
    18661 gacctttttc ttcatatttt gctactgctt gcttgttatg aataagagga cctaaagcat
    18721 atatgccaga atcatttttt tcaacttcat cccatgccat tttcattgct ctttttactc
    18781 caaaacaaaa gccagcattt tttgctattt ttatgttcat aactttaccc ccttattttg
    18841 aatatttaaa gtaattttgt tttatcactt ctaaaatctc ttgtgatatc ctttcatggt
    18901 catcattact caatttttcc ttaaaatgtt cttcaaatga tattggttct ccaatatata
    18961 caattacttt actaaaaagt ttatatttag atattattga tactggaaca actttagatt
    19021 tactttttat tgtaaataca gaaagacctg ccttagcctt accaaaagaa tctcctttta
    19081 ttctagtacc ctctggaaat attcctaata cataaccatc tctaactgac cttaaaatag
    19141 tttttattgt agatacatct ggtttttctc tatttatagg cataacatgc aattttttta
    19201 atataaatcc caatggcttt actttaaaca attctttctt tgcaattgca gctatttccc
    19261 tattttctat ggcagcagcc aagaatatag ggtcaagatt cgatttatga tttgatgcta
    19321 ttactatatt tcctctatct ggaatattct ctgcacctat aacttcatat ttaaaaaata
    19381 ttttagaaaa acccttaaaa atatttatta caaatctata aaaattcacg tttatctccc
    19441 ttcaacacta catttatttt cattatgatg cataaaaaac taaagattta ttttagatac
    19501 aacttcttct acaacttctt ctatagtttt tcctgtagta tctatttcta gtgcatcatt
    19561 agcttttacc aatggtgcaa attctctatt tgaatcaatt tcatctcgtc ttattatatc
    19621 ctcaattact tcttctaaag tagtattata tcctttatta caaagttctt tatagcgtct
    19681 atttcctctt tcttctggag tagctactaa aaagaatttg taatctgcat ctgggaaaac
    19741 atatgaacct atatctctac catctagtat tactgaactt ttcattccta tttttcgttg
    19801 aacttcaacc atcaactgtc ttacttcttt tatttttgct acattagaaa cattattgct
    19861 tacttctatt gttcttattt cttcatttat aacttctttg tccaaatata tattgttatc
    19921 tttaaaatct atatccgaat tttcagctat ttgaataact tctttttcat tattgacatc
    19981 aacaccactt tttaaacact tataagtaac tgctctgtac atagcacctg tatctatata
    20041 atttatattt agcttttttg ctactatctt ggctattgta ctttttccag ctcctgctgg
    20101 accatctaca gctataacta aattacccat atatacacct ctatttattc atatttaatt
    20161 attaaaaaat tatatttata cacttatacc cgcaagatat ccagttgaaa atgctatttg
    20221 taaattatat ccaccagttt ctgcatctat atcaattact tcacctgtaa aaaataagtt
    20281 cttaactaat ttagattcca tagtagaaga attaatttct ttaactgaaa taccaccaga
    20341 agtaaccata ccagtgttta tacctgtagt cccattacaa gttagcttca tattttttat
    20401 atactcaata attctaattt catctgcctt agacaaatca cttgcctttt tatcaactaa
    20461 agataataga gaaaaaatct ctcttaagaa attttgaggt agtatttgct taagattatt
    20521 tagaacattt ttatttggaa atgctctgat tatactagag atttcatcag tacttaaatt
    20581 tggtaagaaa tctatattta attctacctt ttccttttca ataatcttgt taatataaga
    20641 agacattata agaacacaag gtcctgttat cccaaaatgg gcaaatagca tgtctccaga
    20701 ttttgatatt ttccttttct taatcttaca agatatttcc acattcttca tggatattcc
    20761 ttgtaaatct ttaatccatt tttcctctat tgtaagtgga actaaagcag gatatagttt
    20821 gtttaatgta tgtccatgct ttttaagaat cttatacatt gaaccatctg aacccgtctt
    20881 tgaataactc actcctccag ttgatataat aaccttgtct cctagtatct tatctccatt
    20941 atctaaaata atccctttta ttagatactt tgacttgtcc ttattagaat catcttttag
    21001 agcaatttct tcagttataa tatctataac tttcttatta tacattatct ttacattgtg
    21061 attgagtaaa tctttattta atacttctat tacttctata gatttatcat ttttagtgta
    21121 tactttgtgg tcattttctt tttcaatctt atattccaaa tttctacttt caaaatacga
    21181 aataaggtct ttatttgtaa atgtatagaa actactatag agaaattttt tattactgac
    21241 aactttatca aaaaaatctt ctatctctct attattggta aaattgcatc taccgccacc
    21301 agttgcccta agttttcttc ctaattctcc atttctctca actaatatta cttcattatt
    21361 gttttttgaa gctgacaaag ctgccatcat tccagaagct cctccaccaa ctactataac
    21421 tttactcata aatttctcct tataattgat tcctactcca gtgcagaatt atttatcttt
    21481 acatgatttt ttatatgact tgtatcattc atttttacta taacaggtcc ataatcttta
    21541 aattctacta tatttaaagc agtattatct tgcttaattc tatatatatt ttccatacct
    21601 gattctaaaa atgacaacaa cataaccctc aatgtaattg aatgtgtaac aagaattata
    21661 tttttattat catatttttc atttagttcc tttataaaag catctactct ttcctttata
    21721 atcttaagag tttcgccttc tggtatattt actaagtgtg gttcatttct ccatgttgca
    21781 tatatatctg aataatcttt ttgaatttct tttattaaaa ggccttccca aactccgaat
    21841 cccatttctc taagtgcctc agttttttga acttcaatat tgagcttatc tcctaaaatt
    21901 tgagctgttt gcattgctct cccaaggtcg ctactaaaaa tataatcaat tgaatacttg
    21961 ccaatatctt cagaaagttc ttttgcctgt tctatacctt gaggcgtaag gtcagaatta
    22021 ccatgacctt gtgtttttcc caatatattc caatccgttt gtccatgtct tactatataa
    22081 aaagtatttc ccatatgtcc tcctaatata tgcctatttt ttattaaata taaattatca
    22141 cttctcaaaa cttaattata tcatataata ctttactttg tttctatttt ataatctaat
    22201 ctaacatatt atttacaata aatctcattt gtatttctgc aaattattgt actgttaaag
    22261 aatttttcta aatttataaa catatttctc ttatattatt tatatacttc ttcaaatctg
    22321 tattcttgaa ttgaattgac atacttatct gtatctactt tttctaaaaa catatccaaa
    22381 ggtcttgcaa atattcctgt atcatcatat aaggtcttgt ataaaactaa atcattagta
    22441 tcttcttctt ttttatgata aaggtttcca tctaaatccc tataaacata gatatcttct
    22501 tctctttcag tatgtctaat aactaattta tatttatttc tgttttggat taaattttct
    22561 ttaccatata tttcacatat gttttctata tcatcaatag ccttgctaag acccattgta
    22621 gcataatatt tccctttaaa atgtttatag atacaaggat attttaactt tctttcactg
    22681 atacctaagt tattctttaa ttcaatcatt ttattataat ctcccatatc tcgtcccttt
    22741 cttttaaaaa gtattttatt ctgaaataag ttacttgttt tatattttat cataatttaa
    22801 aatttattaa taatacaatc aataaataaa gaggctgtat aaaatacagc ctcccaatat
    22861 taatcttcat aatcatataa tttttctaat ttctttttta atttcttttt tctttctgaa
    22921 cctaaaattc ttataaaatt attattcaca aaaatacaat tattgctgag ataaaattca
    22981 cacttgtcta gatttacaat ataactatta ctgcatttga aaaaattttc ttctttcaat
    23041 agttttctta tacaaggagc attttctcca acttcataat taccactttc agtataaatc
    23101 tttacttcat actcttgctt ttctatgtac aaaacttctt tactattaat tgaaaaacgt
    23161 ttatcctcgt attcaacatt aataaaattt tcctctttca tttccatccc tccaagtact
    23221 tttattaatt taattttagt gttttttatc aatataatct atcggaaata tatgtaagac
    23281 tgatttactt aagatattta aataattatt attaatatta atgtataagt ttatttttta
    23341 acattaattt ttaaagttaa a
//
LOCUS       DAELSY010000057        23057 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.057, whole genome shotgun sequence.
ACCESSION   DAELSY010000057 DAELSY010000000
VERSION     DAELSY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23057
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.057"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(246..1337)
                     /locus_tag="KPG09_001899"
     CDS             complement(246..1337)
                     /locus_tag="KPG09_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966509.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEMTVMKEDISEV
                     KQEMTVMKEDMSEVKQEINVLKGDMSEVKQEMTVMKEDMSEVKQEINVLKEDMSEVKQ
                     EMIVMKEDMSEVKQEMTVMKEDMSEVKQEMTVMKEDMSEVRQEINIMKEDISEVRQEM
                     TVMKEDMSEVKQEINVLKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINV
                     MKKDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIE
                     IKIDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKED
                     IAEIRRDLNLVELATSKNWSDIVKLKSVK"
     gene            complement(1567..2652)
                     /locus_tag="KPG09_001900"
     CDS             complement(1567..2652)
                     /locus_tag="KPG09_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG0966510.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(2688..4001)
                     /locus_tag="KPG09_001901"
     CDS             complement(2688..4001)
                     /locus_tag="KPG09_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0966511.1"
                     /translation="MIAIKSKYLISSADEIYTNSAAVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(4002..6293)
                     /locus_tag="KPG09_001902"
     CDS             complement(4002..6293)
                     /locus_tag="KPG09_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0966512.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDTKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(6283..7077)
                     /locus_tag="KPG09_001903"
     CDS             complement(6283..7077)
                     /locus_tag="KPG09_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG0966513.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(7116..7562)
                     /locus_tag="KPG09_001904"
     CDS             complement(7116..7562)
                     /locus_tag="KPG09_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG0966514.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(7634..9040)
                     /locus_tag="KPG09_001905"
     CDS             complement(7634..9040)
                     /locus_tag="KPG09_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0966515.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(9058..10428)
                     /gene="hydA"
                     /locus_tag="KPG09_001906"
     CDS             complement(9058..10428)
                     /gene="hydA"
                     /locus_tag="KPG09_001906"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG0966516.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKN
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(10561..11748)
                     /locus_tag="KPG09_001907"
     CDS             complement(10561..11748)
                     /locus_tag="KPG09_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBG0966517.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSV
                     YGK"
     gene            complement(11875..13089)
                     /gene="dpaL"
                     /locus_tag="KPG09_001908"
     CDS             complement(11875..13089)
                     /gene="dpaL"
                     /locus_tag="KPG09_001908"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG0966518.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            complement(13452..14123)
                     /locus_tag="KPG09_001909"
     CDS             complement(13452..14123)
                     /locus_tag="KPG09_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0966519.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            complement(14206..16422)
                     /locus_tag="KPG09_001910"
     CDS             complement(14206..16422)
                     /locus_tag="KPG09_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0966520.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHNIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFRGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLIVEEAEKLLETSKEDLYLDNGYVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGLTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVNSYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            complement(16425..16895)
                     /locus_tag="KPG09_001911"
     CDS             complement(16425..16895)
                     /locus_tag="KPG09_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBG0966521.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIKEAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            complement(16957..18351)
                     /locus_tag="KPG09_001912"
     CDS             complement(16957..18351)
                     /locus_tag="KPG09_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0966522.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQLAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            complement(18424..19785)
                     /locus_tag="KPG09_001913"
     CDS             complement(18424..19785)
                     /locus_tag="KPG09_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG0966523.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            complement(20083..21405)
                     /locus_tag="KPG09_001914"
     CDS             complement(20083..21405)
                     /locus_tag="KPG09_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG0966524.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            complement(21761..22801)
                     /locus_tag="KPG09_001915"
     CDS             complement(21761..22801)
                     /locus_tag="KPG09_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0966525.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
BASE COUNT         6871 a         4659 c         2529 g         8998 t
ORIGIN      
        1 tgttctcatt gataaaattt tacttatttc aagcgtttta ttcgatttta tcatctaaat
       61 tttaggtttt atttttataa attaattgta tttacataaa aaaaaagacc taaaaacaaa
      121 tttttaacgg tcttttctca attttttatt ttcatttcta aaatttataa taaatagtaa
      181 gaacactata aattctttta tataaaattc ttagtttgtg gatgataaat tagtgaatta
      241 aaatactact ttactgattt tagtttgaca atatcactcc aatttttact agtggctaac
      301 tctactaaat ttaagtctct tcttatttca gctatatcct cttttataga attagtgtca
      361 ccttttatat atgctatgtt atgttctata ttatcatgag tagctttatt tatttcagaa
      421 ttatgtaata aacttcctaa aatattattc tgttctatct gtatctcttt cattgattta
      481 tcttcacatt ctagactatc aatcttaatt tcaattccat ataatttttc atttgctatg
      541 gatacctcgt ccttaattcc attcatatct tcattaatgt tatttatttt attttgcata
      601 aagtttactt ctgatttaac ttcggaaaca tcttctttca ttacagttat ttcttgtctt
      661 acttctgaca tgtctttttt cattacattt atctcttgtt ttacctctga tgtatcttct
      721 ttcattacag tcatttcttg tcttacttca gacatatctt ctttcattat atttatctct
      781 tgtcttactt cggacatatc ttcttttaat acatttatct cctgttttac ttccgacata
      841 tcttctttca ttacagtcat ttcttgtctt acttcggata tatcctcttt cattatattt
      901 atctcttgtc ttacctctga catatcttct ttcattacag tcatttcctg ttttacttcc
      961 gacatatctt ctttcattac agtcatttcc tgttttactt ccgacatatc ttctttcatt
     1021 acaatcattt cctgttttac ttccgacata tcttctttta atacatttat ttcctgtttt
     1081 acttccgaca tatcttcttt cattacagtc atttcttgtt ttacttccga catatctcct
     1141 tttaatacat ttatctcctg ttttacttcc gacatatctt ctttcattac agtcatttct
     1201 tgttttactt ctgatatatc ttctttcatt acagtcattt cttgttttac ttccgacata
     1261 tcatttttta tgctaactac atcttcattt atagtatata ataactctaa aatcttctct
     1321 tgcacttgca tcaccatccc tattaattat tataacattt tacacaaatg ctatacaatg
     1381 ttaacatata tatcagaatt attaaaattt taattaaaca aaattgattc actttattac
     1441 ttcaaaacta attaatatct aaaataacta caaataaaca aaaataaggt caacaacaaa
     1501 aaaggcttat ctctttgaga tagcctttct tatttttatt taaaactttg ttttttataa
     1561 aataatctat acattcagct ctgaacatga aatttttctt ccaagtgctt ttggtttaca
     1621 aactgatata caaagtccac aatatctaca ctcttcttca ttcaattcca tcttatcatt
     1681 caattttctc gcttgatatg cacaaactct ctcgcacatc ttacatttag tacatttttc
     1741 ttcatcataa gtaaactcaa agctaagtct ttcattaaca tctcccatat gattatgtac
     1801 tataccacaa gcattttcta tagtattata tcctaatcta ttaagattag attttaaatc
     1861 atcatgtatt ttagatatta cttgtgcgcc ttttaaaatt actactgaac atataccaac
     1921 aaagtcagca cctgccataa tcatttctaa ggcatcatct ccacttacac atccacctaa
     1981 gccaattatt ggtatattaa cttgtttttt tatttcatat attctctgta aagttattgg
     2041 cttaatagtt tctccactta aatatcctat tccatttcca cctaataaag gttgccctgt
     2101 ttctatgtct attctaaatg ctggtcctac agagtcacaa gcagttattg catctgctcc
     2161 tgcttcttct aattttttag caaaatcgcc tatctcatct atcataggag gaagtttaac
     2221 tattacaggt atatttactc tttcctttgc atctttaagc attggtatca aatctctata
     2281 gtcataagat actaattcaa taaagtcagc tcctgcattt gcaactttct ctactaattc
     2341 gctactttct tcaattgtat gacctactga agctatacag acagtcccat ctcttttcat
     2401 ttgtggtatt tcaaaatcta tccattgctc tggctcaaaa tctgtccatt tttcattatt
     2461 taaaagagca ttagctgtat ttctaaccat atgtggtgct ggatttatag ctctttcttt
     2521 tcttatagtc ttagtcacaa ctgcccctgc tcctgcatca cttaatatct tgcatccagc
     2581 tgcactatat gttaatggtc ctgaacctat tataagtggt gattttaatt ctttacctaa
     2641 aaaattatac attatctgct tccccttttc atatatattt acattcgtta aaatttccaa
     2701 aattcacaag caacttcttt catttcttta acaatagctt ttttatctaa atcaactaac
     2761 tgtttttctt ttaaaatatg tctaccatct atgtatacat ttgatataaa ttctttttta
     2821 ccctgaacta ctatctggtc aaatatatta tcaagagtca caggagtttt aaattcatct
     2881 tccataacta ttatatcagc cttattacca actgctattt taccagaatt ttgaagtcct
     2941 aatacataag caccatgctc tgttgccatc ctaaatacta aatttgctgg cattactgat
     3001 gcatcttctt caactgattt atgcattaaa aatgcacctc tcataacttc aaaaaaatca
     3061 tttatatatc catcagtacc cagtgccact tttatgcctt ttttaatcat ttttggtact
     3121 ggagaaaaac ctccacctac ttcacagttg ctaagaggca tatgaaccac ttttacacct
     3181 ttttctttta atatatctat ttcttcatca tttactttta cacactgaga tgctaataca
     3241 gtttcatcca atatatctaa atcattatag tagtcaactg cttttttgcc aaaatgtttt
     3301 tcagcatagt ttggctcata tatactctca cataaatgaa attgcattgg tgcatttaat
     3361 ttttttgcat cttctttagc tttcttaata aatctatcag aacatgtgaa gcttgtatgt
     3421 gtacacatta ttccatttat taatttacta ttttctctac tccactttat taaattagaa
     3481 ttttcatcta aacatctaag tccattttca aagcttattc tttcacaact ttctaaggat
     3541 aaaatcgctt tcatgcctat ttcttctagt atttttcctt gctcaattaa agttccttct
     3601 tctgtatttg gagcttctag ggtatcacaa aaagctacca caccagattc tataagttct
     3661 attgctgatg ctttagttgt tgccttcact tctttcaatc caattctatt ttctataaat
     3721 ggccaccaat agtcatttaa aaaaccttca aagtcagtaa aatgtacatt tgccggtatt
     3781 cccctagaaa gaactccata ttgatgcata tgaccattga cgaatcctgg cattattata
     3841 gcatctgatt tatctattat ttcttcaata tttttatatt ttatttcaac ttcttcattt
     3901 ggcaatatat cttttataat gtcattttct actacagcag cagaatttgt atatatttca
     3961 tcagcagaag atattaagta tttactttta atagcaatca tttatttacc tccctgattt
     4021 aattttttca tggccatata aactttctca gaagttatag gaagttctct tatacgaact
     4081 cctgtggcat tgtatattgc atttgctatt gctggtgcag gtgtatgaat aactatttct
     4141 cctaaagatt tagctccata tggacctgtt ggctcataat tatctacaaa atcaacaatt
     4201 atgtttccta tatctttttt tgttggaact ttgtattgta atagattaga agtttgaagc
     4261 tttccatcag aaccatgtct agggtcttca tacattgcaa gccctatacc ttgagttaca
     4321 ccaccttcaa cttgtattct agcaagtgca ggattcataa ctgtaccaca atctgtagaa
     4381 caagcataat ttattacttt aaactcccct gttgttttat ctaactctat ttcagcaaat
     4441 cctgctaaga atggtttagc actctcttgt ataccataag attcagatga atataaaact
     4501 tcttgatttc caccaccaac agattctttt cctaattcac ttaacaaaac aaacttctct
     4561 acatcttcac tataacaaac tctgtcttca tatagtatta aatcatctat tggtaaatcg
     4621 agtttcttct gtgctacttt taatatcttt tgttttaaac tttcagcaga ttttattgta
     4681 gcatttccag ttacataagt agtacaagaa gcgtatgcac cagtatcata tggacacata
     4741 tctgtatctc ctgtataaac ggaaattcta tcaatagatg tattcaatgc ttttgcagct
     4801 atttgtgcaa gtattgtgtc agaaccagtt cctaagtctg aagacccaga aaacaactta
     4861 taagttccat cttcatccat cctcatattt actattgcca tgtctatgtt ggctatacca
     4921 gaaccatgag ttcccacaga cattccaaca gccctcacct tattgtttcc aatatcccta
     4981 acagggtact tttcatccca tccaataagt tttttacctc taattataca ttcatcaagt
     5041 gcacaacttc ttattggaaa attcattatt ccgccttcag tttgtttttt tataatattc
     5101 tttaacttta tttctgttgg gtccatatta agtttatgcg ctaattcatc aattgcacaa
     5161 tcaagtgcaa atgtaccttg tgttgctcca tatcctctta atgctcctcc tggaactaaa
     5221 ttagtatata cagttctccc atcaaatttt attgcaggta cattattata tgttggaagt
     5281 acattatgac ctgattcaga gcaaactgaa ggtccattat caccataagc gcctgtatta
     5341 ttcaatgctt tcatatctat agcttttatg tttccttctt tatctgcccc aatttttata
     5401 tcaaaaaaca tctgatgtct agtattagtc attgcaaaag tttcttctct agtataaatt
     5461 agttttgctg gtctttttgt catccaagtt acaaatgatg catatatctc tgttacagct
     5521 atgttttttc caccaaaacc tccacctatt cttggtttta ttactctaac ttttgaaaga
     5581 ggtattccca gtgctttaga aacctgtctt ctcatatgat aagctgattg gtttgcagaa
     5641 gttactacta atctgccatt cgtatctata tacgtataag ctctatgagt ttccatcata
     5701 caatgggctt gtgattgagt catataagaa tgttctacta ttacatcact ttctttaaac
     5761 tctttttcta catccccttt acccattttt tctctcgata caatattttt tgtagcatcc
     5821 aatccaaaat caaatggaca aaacaaatca tcttcttcat ggattaaaat tttatttcct
     5881 tccgattctc ttgcatccaa tataggagtc attacttcat attcaacttt tataagtttc
     5941 atagctttaa tagcagccct ttcatcttca gcggctatta tagcaacttc atctcctaca
     6001 tatctaacta cgtcttcaag tattaattgg tcatgaggtg atgcttctgg atatgactct
     6061 ccaaccattg aatatctata atttggaaca tctttgtaag tgaatatgtt tactacacct
     6121 tttaccttta atgcattttt tgtatctatg ttttttatct tcgcaaaagc atgcggactt
     6181 ctaagaagct taattgttaa aacatcttta tgaaaaatta aatcttctgt atatattggt
     6241 tttccagtaa caatggcttc actatctatt tttcttacct ctttatttac catcacactc
     6301 acctccaata ctattataca ttctcactac taaggctttt gccatatctt ttctgtattc
     6361 tttacttcct cttatattac ttccaaatga aagttcttca cttgatactt caccagcttt
     6421 ttctatatca ttttctaaac ttaatatttc acttgcttta taggcaattt ttgctcttct
     6481 tggtctagcg cctatcgcaa ttttataaat acttccttct tttaacattg ctccatttaa
     6541 tactgcaaaa tcacctgtac attttcttat agaatcaaat acacctatag catctttttt
     6601 aggtaatata acttctatta atatatcttt ttcaagctca ctttctaaaa aatcagctaa
     6661 gttcataacc ccttttttat ataacttaac ctttgcatct accactagta atgaaggtat
     6721 taaatctgaa aatccatact tagcaaatac gctagctcct actctggctc cacttctaaa
     6781 ttgaactcct actatattag aaacagcgtt tgaaattacc ccattacagt aatttttaat
     6841 aaccttattt aattctaaag ttcttaaact tgtgtctgct ccaattaata tgctatcatc
     6901 agtttcattt atataatcta aagctaagtc ttttaaatct attgctgagc caatattttt
     6961 actacctagt tttaaaaatc cacatcctcc aagtattatg ttgttttttc ttgatattag
     7021 cagttcataa gcttcatcta aactttttgg aactacatat tcctttatag ttatcatact
     7081 ctctccccat atcaacttaa ttttcactta aaagtctaag tgtttaaata tttatgtatt
     7141 gctcttaatt gacctttata tcctgtacat ctacaaaggt taccatttac ataatctata
     7201 acttcttctt ctgttggatt ttgaagctct cttttcattg ccaaaaccat cattataaat
     7261 ccaggagcac aaaatccaca ctggtcagcc ccttcgtctg caagaaaatc tccaaacttc
     7321 tttgcttcat ccaagacccc ctcaagtgtt gtaatatgct gaccatctac tctagcagta
     7381 agataattac atgaaagcac ggccttgtca tttaccatca ctgtacataa cccacatgag
     7441 cctgtatcac accctttttt aacacttaca tatccatttt ctcttaatgt atctgctaaa
     7501 aaatcatctg gtcttatact taattctttt tctttattat ttatggtaca tttgataatc
     7561 aaataaatca cctcaaaact catattttta agctaaagtt ttactatact tttctatgaa
     7621 taaattacat ttattaataa aggctattcc atttatgacc ttccattcta ttttttatag
     7681 ttcctcttac acaaagtctc tcagctgtat cttgagcatc ttttaatgct ttctcttcat
     7741 tagctgtcaa tatttttgaa tcttccatta ttatatttcc atctactata acactctcta
     7801 tatttttcat actagaagaa tatactaatg tagataccgg attatgcata ggtatcgctt
     7861 ttggtgaaag cattggatta aatattagta agtctgcttt tttacctatc tccaatgaac
     7921 ctatctcatc ttccattcca atagctcttg ctccatctat agttgccaac tcaagaactt
     7981 tttctgccga cattgctaat gggtcacact tattaacttt atgttgaagt gctgttaatt
     8041 tcataagttc aagcatatct tgagagttat tactagcggc tccatctact cctaaactaa
     8101 ctgttattcc ttttttaagc atttctggca ctggtgcaac acctgaagat aagtacatat
     8161 tacttgctgt attatgagac actttcatat cgtatttttt agtaagttcc atatcttttt
     8221 cagttagata cacacaatgt accataagca catttggtcc aagtattcct aatttttcta
     8281 aaacatctat gtcatattgc ccatgcaatt cttttgctgc ttctctatca aaaggagtct
     8341 ctgatatatg tactgtaaat aaagcatcat catattcttt taccactctc catagcattt
     8401 caagcatttc ttgagaattt gaccatattg ctgcaggagc tactcctatt ttgattcttc
     8461 cattttctgt attatggtgt ttttcaaaca atcttcttac atctttttca accgtttcta
     8521 catcttgcat tattccagga tgaactccaa attgagctcc tgtattcata catcctcttc
     8581 caagtattcc tcttattcct aattctttat aagcatctat tattccatca caaagacctg
     8641 gcttactatg aggatacatg tagtcaacca tggtcgttat accacttcta agtccttcaa
     8701 tacatccaag cattgcagca tcataagtat cttttggctc taaatagtta gctgctggaa
     8761 atgtcatagt ttctaaccag tctttaagta ccatatcatc tcccaatcct tttaataaag
     8821 tttggaataa atgattatgt gtatttataa aacctgggaa taaaacttta cctttagcat
     8881 ctataatttt tttaacatca gtatatttac tttctatctc tttagaattt ccaatatcag
     8941 caatcttgtt atcttttact actaatgccc catcaaaaat aacttctctc tctttattca
     9001 cagttactat tatggcattc ttaataagta tatccatcat tcttatttcc ccccgcatta
     9061 tttagtaatt tttcttttaa tatattgacc tgttggatgt tctttaacat tacaactttg
     9121 tactacatga tttcctctca aaaatacatc ttcaacttta catttaacct taaatccttc
     9181 atatggagta taatctacat tttgtttttg agttttataa ctaattgttt cttctttatt
     9241 gatatttaaa atgactatat ctgcatcact tcctacagct atctcacctt ttttaggata
     9301 cattccaaat atttttgcag cattcgtaga agtaacttcc acaaacttat ttacacttat
     9361 tctattttct aagaccccat atgtatataa aagtgctaac ctatgttcaa caccaggtgc
     9421 tccatttggt atcttactaa aatcatcaat tcccaagtct ttttgcccct taaaattaaa
     9481 tgaacagtgg tctgtgccaa ctgtttgaat atctccacta gacaatcctt cccaaaggta
     9541 atttatgtct tctttttttc ttaaaggagg agacattaca tacttagcac cctcaaattt
     9601 attcttatta tataaattgt cttctaataa tagatactgt gggcatgttt ctactatcat
     9661 atctatattt ttttctctag ccatttttac agtttcaagt gactccctac aacttaagtg
     9721 gactacatag gattttgaat tagataattt agttatatca gcaagtcttg aaacagactc
     9781 tttctcaact atgttaggtc ttgtaagtgg atgatatttt ggctctaaat taccattttg
     9841 tatattttct tctatcaata catctagtaa atccccattt tcacaatgaa atccaactat
     9901 acaacctaaa tcccttgctt tctttaaaac cctgtatatt gttccatcat ctactttcat
     9961 gccatcatat gccatataca tcttaaaaga tactatccca tccgaaataa gtttttccat
    10021 atgattaaat gtatctttat ttaaacatgt aattgtcata tgaaaaccat aatcacaata
    10081 acaatttcca cttgcttttt tatgatatgc ttctacacct tgcagtaggt tttccccctc
    10141 attggcttct gcaaaatcta gtattgtagt agttcctcca gctatagctg ctcttgttcc
    10201 tgtttcaaaa ttatctgcag ttgtaataga acctgcattc atatcaaaat gagtatgtgt
    10261 gtctattcca ccaggaatta caattttacc tgtagcatca ataaccttat caccatttat
    10321 ttctaagtta tttcctattt caactatctt ttcatcttct atccttaaat cacttatata
    10381 agtcttatca gaggtaatta tagttccacc tcttagtatt gttcccatat atacctccta
    10441 acttttaagc tttaaagtat tttctaagta tcgtaaaact ttatttaaaa ttgtattttc
    10501 taaaggtgct atattcagca cctctagact atatttaaat aatttattgt gaactaaata
    10561 ttattttccg tatacagatt ttaatattcc atagtaacct tctgttccta taaataactg
    10621 ttcttgctct atatactcat ctattgtgtg agcaagattt tccttagaag gaccaaatcc
    10681 tatagttttt attccagctt cacctgcata gtgacttcca ttcgtacaga atgagtattg
    10741 agttatttct gaatctattc cagcttcttt taatccctta tatgctgctt gaacaaattc
    10801 atcttcttcg tcatataacc aacctgggaa gaatctttca ctttctatag tatttcctgt
    10861 atagcaatca gctttttcaa ccgcataaga aactttcgca tttaattctg ggtcttcttt
    10921 catcatttca tctaataaag cttgtattgg agctaatacg ccttctcttg tttctccaac
    10981 taataatctt ctatcaaaag tagctttgca ataatctggc actactgatg cccctggata
    11041 tggagaagat tttatatctg ttaaaactaa tattccatca cctaaaactg gatgagttgg
    11101 aggtactaat tgttgtatct tttggattac attagccatt ttataaactg catttacacc
    11161 tttttctgga ttagcagagt gagctggttt accaaaagtt tctactacta tttctcctct
    11221 acctctctga ccaatcttta agtttaactc tgaagactca cctattacaa catagtcagg
    11281 ttgaacagcc ttacttatct ctctagccga aacaccttca aatatttctt catgaacaac
    11341 acctgctaca tatagttctc cagcaaaatc tcgattagtg tctttagcaa aatacgctac
    11401 agcagacatc attgcagtat attgaccttt catgtcagaa gttcctctac catatatctt
    11461 tccatcaact atttttcctt caaaaggagg aacactccat ttacttgcat ctggaactgg
    11521 aactgtatct atatgtccat caaataatat tttcttacct ggcttgttac cttttataca
    11581 tcctactata tttccatatc tatctcttga ccaactatca aaacctaatt tttcaaaaga
    11641 ctcttctatt gcttttacaa caccttcttc atttccagaa gaactaggat tttgaattaa
    11701 tttttggcat atttcagtta cttcttgttt tctttgttcg tttaacattt cattatcccc
    11761 ctagattttt tataatgtat gtattttata tacttatata ttttctaaat tttagaactt
    11821 atatatcttc taaatatgta aaccaatatc taataattgc attttagtaa ttccttatat
    11881 acttggatat tctccatccc aaactatact tctatatttg tcagggtcag tatctccttc
    11941 tgtgcttatt aaaagtattc ttgaattttc atctaatttt aaatcttttc ttaaatctgc
    12001 taaatcttct ctttccatag ctgctactaa aagaccagtt gtaactgctc cagattctcc
    12061 agaaattacc ttttcatcac catttaaagg attacctaat gttctcatac ctttagcaga
    12121 aacccagtct ggtgctgaaa caaatgctgc agcatgattt tttaatactt caaatcctat
    12181 agtatttggt tctccacaag caagaccagc cattatagtt ggcatatctc ctccaacaaa
    12241 tcttggtttt ccatctcctg cttctgctga tttatagtag caatcagctt catcagcttc
    12301 tactactact gttattgggc attcatcgta tattgaagca acatatcctt gaactgctcc
    12361 agcaagtgaa ccaacccctg cttgcacaaa tacatgtgtt ggtctatcta ctccatattc
    12421 ttttaattgc tctatcgctt cttgagccat agttccatat ccttgcatta tccatgctgg
    12481 aatttcttca tatccatccc atgctgtatc ttgaaccatt actccatgat catcagcttc
    12541 tgcataatca ttagctaatc ttactgcatc atcatagttt aagtctgtta tactagcatc
    12601 tgctccttct cctttaattt tatttaatct aaactcagaa gaaccttttg gcattaatac
    12661 aacagatttt tgacctaatt tattagctgt ccaagcaact cctctaccgt gatttccatc
    12721 tgtagctgta aagaaagtta tttctcctaa tttttctttt atttctttcg aagttaattt
    12781 gtcataccct aactcagata tatctgtatc taatctttga gctaagtatc tacccataga
    12841 ataagaacca cctaatactt taaatgcatt taatccaaat ctataagatt catcttttac
    12901 atatactcct gcaacaccta atttttttgc taaaccttct aggtttgcta atggagtttc
    12961 tttatattga ggaaagcttt tgtgaaaatt tctaacctta gttatttctt cttcatttaa
    13021 aaatttgata caattttcct tatcaccctt tggcaaatta tttactttcc atttaatttc
    13081 ttttaacatc taaaatacct ccaaattatt gttaaatttt tatactttaa tatataagca
    13141 attaacatgc caacactaaa tatatactag atatttttct atgactcttt agtatagaaa
    13201 ttgcaatgct tttattattc tttcttttct atctttagtt catacaatgt atgtagaatt
    13261 taataactaa aaaacctttt ctatcttctc actttttaat tctcattttt aaaatttaaa
    13321 aataaatctc aaaacacttt gtttctatat tttttaatat aaaatttata aaatctaaat
    13381 taaaacataa aatagtgatg caataatcta ttacatcact atttgtatat atttaataat
    13441 ataataaatc tttagtggtc tctaagaggt gatttttcat cttttttaac atctaattct
    13501 tcttttacga cattccattt ttcaagacca aacttagaaa ccataactgc ctctgcagca
    13561 ccttttccaa gtctttttac tgtgtccata cctgtctcat cctcttgggc accttttgct
    13621 tttccatctt tactccatat tgtaccacct atatagcatc cacctaaacc tccagatact
    13681 aacatttcat gcattaggaa gaaatttatt ataggaagtt ttgtcaattc ttggccacca
    13741 tatctagttc caccagtagt tatagctgca ccaaccttat tttgaagttt tcctggatga
    13801 actaaataca ttggacgaag tctattaaat actgctgtaa gttgagcagt tatattcata
    13861 tcataaactg gagaacctac tataattcca tctgcctcca atattttttg ctctaattct
    13921 tggatatcat cttttataat acacatagtt tcttttctta tacatgcatc acaatgtaca
    13981 caaggactta tttttttccc tctaactgac caatactcag tttctatacc aggtatagtt
    14041 tctgcttctt tcaaagcttc taacactgca tattctgttg ctcctttacg tggacttcca
    14101 caaataccta atatctttgc cataataata tctcctcacc aaataaatct atttttatta
    14161 tctatttaca aatacaataa attgttgttt gtgctttaat ttctactatt ttaattttat
    14221 tggcaatgaa tgtatttcta catcagttgc attatatact gcatttgcaa cagctggcgc
    14281 aactggtaca acagcacatt ctccaatact cttaccacca aaaggtccag ctttttcata
    14341 actatttaca aatgcaactt gacattttgg catatctaca gttctaaata atttatatgt
    14401 tttaaaatca gtagctttag gtctaccttg ttcatcaaat gccaattctt cactcaatgc
    14461 gtatcctaga cccatatgaa tcccaccttc taattgacct tctaaattta aaggatttat
    14521 aactctacca acatcatgta cagctacaaa atcaactact tttatatcct tagtttcttt
    14581 atctactaaa acttcagcaa aatgtacacc ataagaagta agtccagctg gtgaagaata
    14641 atcttcaaca acagttaact ctcttaatga tttactttga ctatatactg cgacatcaga
    14701 aagtgatgct tttacatttt catcaagttt actaattaca tatccattgt ctagatataa
    14761 atcttctttt gatgtttcaa gtaatttttc tgcttcttct actataaggt ttttcaattt
    14821 ctctgctgtc tttttagctc ctccaccttc tacaaatacc cctctacttg catagtcacc
    14881 aaggttgaat gtacatgcat ccgtatcagt ttcaaatgta tcaactttat caggtgttat
    14941 tcctaaaact tcagctacca tcattgtttg catagttacg acaccattac ccatatcatg
    15001 tgctcctgtt agtagtgtca atgtaccatc ttcatttaat ttaagagtca atgtaacaac
    15061 atctctatgt gcaccaaaaa ctccattacc atgtgcacct attgccatac ctatacctct
    15121 taaatatttt ccttcttctt ttggaagttt tttcttttca tcccacttaa acatcttctt
    15181 accttgctct atacaatcca atactctagg atttccaaga gaaccgcgga agattatatc
    15241 gacatcatct ggctctactg catttttatt ttgaaaatcg actaaatcca tacctatttc
    15301 cttggctatt ttcgcaaatt gagcctgttg agccataaat atttgaggtg acccatagcc
    15361 tctcatagct cctgctattg gtgtatttgt atatacaggc atcccagtaa actttatatt
    15421 tggtatttta taaactttaa atactttatg actaagtgct cctataacat ttaaagcact
    15481 tgatgcatat gctcctgtat ttgtatatat ttttatatct tgtgctatta cttcaccatc
    15541 tttatttaca ccagttttta attttacaaa agacccatga cgagttcttg ttgatatcat
    15601 agattccttt cttgtaagaa ccattttaac tggtcttctt gtcatttttg ataatgcggc
    15661 tgctactggt tcaattgtaa gttctaattt tccaccaaat gagcctccaa tagcaggtct
    15721 tataactctt actttattta atgacatccc aaaaatttga gatagtatta ttctaaatcc
    15781 aaatgtattt tggcaaggtg tatgtacagt taatttatca ttataatcat agtgtgcaat
    15841 tacactatga gtctctattg cactatgatg tattgctgga gtagagtatt catcttcaaa
    15901 tatatagtca cattccttga agcctttttc tacatctccc ccatttatgt tgttttgaga
    15961 aactatattt gaatctccat gtatgcagat agcatcttct tttattgcat cttgtatagt
    16021 aaatacagct ggcaattctt catattcaac ttttataagt tttacagctt tctctgctat
    16081 ttctggagtc tctgcagcta ctgctgcaac acggtcacca acaaatctaa ctgtatctga
    16141 aaatataact tcattttcag gtatattgtg ctcataaaat cttaatgaac cattgtattt
    16201 tctttgtgat gtattgagat aagtagctat agctctaaca cctactactt tctcagcttc
    16261 acttgtatct atacttttta tttttgcatg tgctacagga cttaaaagca tcttagcgta
    16321 aagtacattt tgtggtttca tatcagccac gtatttcatt tgaccagtaa cttttaaagc
    16381 tgcatctttt agagggtatg attttcctat taccttttcc ataattatct accccctaat
    16441 ctagctgcag ataattttac agcttctata atctttacat atcctgtgca tctacagaga
    16501 ttttctttaa aagcttcttt aatttcatct tcattaggag atgcatttct atttaaaaga
    16561 gcttgtgttg ttataatcat accaggagta caatatccac attgtacagc tccaacttct
    16621 ataaaagctt cttgtattgg atgaagattt tctccattag caaaactttc tatagtgtat
    16681 ataactttac cttcgcagtt ttttgcaaga agtttacaag atttcatgtc aactccatct
    16741 atcaaaactt tacatgctcc acaactgcca ctattacaac cttgtttcgc tccagttaga
    16801 tttaattttt cacgaagtac atgcaaaaga gtttcattct tttcaacttc tatttcatac
    16861 atgtaatcat taatatttaa ttttattttt tccattttct cctcctacag ataaactaat
    16921 gttgtaatag aatattatta caatcttatt ttaatattag tataagctat tccacttatg
    16981 tccttccatt ctattagtta cattagctct cttacataat tcttctgctg caagttgtgc
    17041 attaagcaac acttgttctt cactttcaat tgtagttact tttccatttt caagtattac
    17101 atttccatca actaaaacac tctctacatt gtgcatacta gctgagtata ccaaagttga
    17161 tacaggattg tgaagaggta ttgattttgg agaaagcatt gagttaaata ttactaggtc
    17221 agcttttttc ccaatctcca aagaacctat ttcatcttcc attctaagag ttcttgctcc
    17281 ctcaattgta gccatttcaa gaactttctc tgcagaaatt gccagagggt cacaattatt
    17341 tactttgtgt tgtaaagctg ttaatttcat aagctctacc atatcttgtg cgttattact
    17401 tgctgcaccg tcaagaccta aactcacatt tatacctttt tttaacattt ctggcacagg
    17461 tgcaacacca gaagcaagat acatattact tgctacatta tgagaaactt ttatgtcata
    17521 ctttttaagc atttccatgt cttcatcagt tatacataca caatgtactg ctacaacttc
    17581 tggacctact ataccccatt tttcaagtaa ttttatatct atttctccat gtatatcctt
    17641 tgttgctgtt cttgcaaatt ctgtttcaga tatatgaact gtaaaatatg aatcatattc
    17701 tttaactata ttccaaagca tcttacccat ttcttcagat atagcccaca ttgaagaagg
    17761 agctacacag atttttattc ttccattttc tgaattatga tatttttcga atacccttct
    17821 caaatctttt tcaaccgttt ccacatcttc tataagctct ttgtgaatac ctaaatctat
    17881 acaacctcta cctacaaccc ctcttatacc taaatcttta tatgccttta ctattccatc
    17941 tgtaagtcct ggtctattgt gagtatgcat ataatcaacc attgtagtta taccactttt
    18001 taaaccttcc atacatccaa gcattgcagc atcataagta tcttgttctg ttaagaattt
    18061 tgctgatgga aacatcattg tatttaacca ctcatgaagt gccatatcat cacctaaacc
    18121 ttttaataac acttggaaca ggtgattatg agtatttata aagccaggga atattacttt
    18181 tccattagca tctataactt ttttagcatc aagatactta ggctcaatat caactgtatt
    18241 tcctatatcc aagattctgt caccttgaat agctattgct ccatcaaata taacatctct
    18301 gtctttatta actgtaacaa ttattgcatt ttttattagt atatcaatca tcttaaatcc
    18361 ccctttgaat actttcatta caacatttta ccatatggtt ttctcatatt atatctaata
    18421 catttatcca atattttcct caagatttga agtaacgttt tcagcacttt tacgtgactt
    18481 taatttaaat aatgtttgaa gtaatatagc tattattgct ccagaaacta tacttgaccc
    18541 tccaaggaca gttccaattg tttctgggaa ttggtctaaa acttgtggag cagttgtaag
    18601 acccatactt gttgctatag atacacctgc tataagattg ttttcttcag tgaatccatc
    18661 catactcata atagagaaac cagacattgc tattgatgag aaaactacta aagttgctcc
    18721 accaacaaca caagatggta tagttgtcat tattgcaccc aatgctggag atatccctgc
    18781 tagtaataat acaagtgccc ctagtgctat tacaaatcta cttgtaactt tgttcataga
    18841 tacaattact gtattttggc taaatactcc agttggaaga gctgcaaata gtgatgaaat
    18901 tatagaagtt atagaatttc ctaaaacaac tgatgctaat tcttcgtcag ttacctctct
    18961 atccattgct cctactgtag ctattgtaca ggcacccatc atatcagcta tacctattat
    19021 gtaaataata gtaaacatta ctattaactc tggtctaaat tctaatccaa aagctgctgg
    19081 tgttggtaaa gatactatag ctgctccttg aacggctgag aaatcaacca atcctaaaac
    19141 taaagatata gcataaccta ctaatattcc tattaatatt gaagaatttt ttactaaacc
    19201 tttaccgtat ttatttatca ttattattac aaaagcaact ataactccta ctgtgaagtt
    19261 tattgggctt ccaaatgttt gtgtgccttc tcctccagct aaattcttta tagctgttgc
    19321 gaatagtcca agtcccatac atgctattat agttccagaa actattggag tgaatatatg
    19381 tctaattttc ttaactccaa atcctacaaa gaatacgatt accccagcag caatctgtgc
    19441 tccaaataga gctgctaaac catctttagc tgctactgaa agacaagctc ctaaaaatac
    19501 ataactcatt cccatcatta caggtaatct tgaacctaac ttaaatcctt taaataaagg
    19561 tattggatat aactgtaaaa acgttgctaa acctgctgct aacatagaac cctgaatcaa
    19621 cattgttgct tgcccctggt taagtcctac cacgtttgct attaatatcg caggtaccat
    19681 atttcctact atcattgcta acagatgttg taaggccaaa ggtattgatt cctttagtgg
    19741 tggtatccca tcgacatcat attttgaagt attacgtgtt ttcataaaat aaatcctccc
    19801 atacataaat atcttgacta aatattttta taattatttt ctataaatat ttttatgacc
    19861 atgaatttat tgctcaaccg ataaacacag ctaaactgag ctatttttat aaaaaattaa
    19921 caaatttcta aatattccct ccccgatttt caacttaaat gaaacattaa ttgcctggta
    19981 tatatttatt tgttaaattt tttattaaaa tgaatagcta tcaaaaataa tctaacaatc
    20041 tcatactaaa taatttaaac aattctttta aattattata tcttaattag tctcttccat
    20101 aacagctctt tctttagcct catcctctag acttttattt ggtattatta gatttaaaac
    20161 gaatgcaacc aatgttgcaa ttactattgg agattctcca aatactaact ttacaaatga
    20221 aggaaatgcc tcttgggctg ctggtactga tgttattccc atgcctaagg caattgccaa
    20281 accaacaatg gtaatatttc ttgatgacaa ctcttctgta ataattaact ttatacctgt
    20341 cattgttatc atactaaata tagatattgt agctcctcct aaaacagaat atggtatagt
    20401 agtcattaat gcaccaaatt taggcacaaa ccccgacaat aacataaata taccagctat
    20461 tccaagcaca aatctactta ttactttgtt catagctacc atccccacgt tttggctata
    20521 tgatgatggt ggtaaacctc caaacaaagc tcctatcata gtacaaattc cacttcctat
    20581 tactccactt gataattcct tatctgtgat attcctatcc atgcctccca ttgtagtagc
    20641 tgataaatct ccaattgctt gaacagcatt gattatactt acaattacta ctgagattat
    20701 tacaggcata tcaaattcca tcccaaattc aagcggttta ggtatcgcaa accaagttgc
    20761 ctctctaata gggtcaaaat ttattatacc cagaactaaa gatacacaat atccaactat
    20821 aattcccaaa aatattgctg ccaattttgc atatcctttt gcaaattgac taagacctat
    20881 aactaccact aatgttatag ccgctactgc ccagttttgc atagaggcat aagtaggact
    20941 atttacacca cctgccatat aatttattgc tataggatac aaagataatc caattgtaaa
    21001 aaccactgtt cctgcaacta tattcggaaa aaattttctt atctttttta tgaatatccc
    21061 tattaatact gtagccactc cccctattaa ttgtgcaccc aaaatgcttg ctaatccata
    21121 atttcctccg actgctacaa gtgttggtac ataagtaaaa ccaactccaa aaattatagg
    21181 caatcttgaa ccaatatttc ctattggata tagttgtata agtgttgata taccagctat
    21241 tattaatgca tattgcacta aaagtgttat ttgtgtaggg ctaacaccca cagctcctcc
    21301 tacgactata ggtactgtaa cagttccaac tatcattgct agtatatgtt gcattgatag
    21361 tggcaaacac tttaaaattg gtggccttcc atcaatatca aacatagaaa cattcttttg
    21421 atatttactc aaggttatca ctcccaacta ttttttaatg acagttatta ttatctcaca
    21481 caacatcctt tcttttttta ttttgtcatc tcaatttaat atgaagcaat tataatgcca
    21541 attttttaac ataattgttt tacatatata atttttatta tatttgttag tatttgataa
    21601 tttattaaag catttatttt tttaaattct tttaaatttt ttttattttt ggataataaa
    21661 atgctattac acaaaaaaac acttagaaaa tagttttcca agtgtttttt atcaatttta
    21721 taatttcaaa ttttagaatt gagaataaaa aacctaatta ttatacatct atttttctgt
    21781 acaatgtagc tagtgatatt ccaagttttt tggcagccag ttttttagac ttagtatcat
    21841 ttccgtaaaa ctttaacact ttctttatat aggctctttc aaactcttca agtgtaagta
    21901 tgtcttgttc catacttatc tcatagtcac tttcctcaag taactttata tttatattat
    21961 ttaacttaaa atattcaagt atctttctat ttattattcc tttatgtatt attccatctt
    22021 cctcaagaag gtttaatgcc agttctatag tattttccaa ctctctaata tttcctggcc
    22081 aaggatactt aagcaacatt tgttccacat ctggttctat atggtacacg tttttcttca
    22141 tttttctcga atatttgtct ataaaattat ttattaatat ttgtatatcg tctcctcttt
    22201 ctctaagtgg aggcacttct aaaggtatta cattcaatct atagtataag tcttctctaa
    22261 atttattttc cttaaccaac tcaaataaat ctctattagt agctgatatg attctaacat
    22321 ctatatcaat tagttcattt gaacctattc tcgtaaactt cttttcctgt aataccctca
    22381 ataactttga ttgtagttga attggcatat ctcctatctc atctaaaaat acaacccctc
    22441 cattggccaa ttcaaactta ccaattttac cttttgaatc tgctccacta aaagctcctt
    22501 ttgtatatcc aaaaagttca ctttctagta aaggttctgg tatagcagca cagtttatag
    22561 caacgaatgt tttattccct ctgtttcctg cgagatgaat ggctcttgct attaattctt
    22621 ttccagtgcc actttctcca gttatagcta cagtagaatc actatttcca attttgcaaa
    22681 ttctattctt aagagcacgc attacatcag actctccaat tatggagtct actgttgttg
    22741 ttccccaaat attatatact tccattccac catacattac tgaatcactg tgtttgtcca
    22801 taaaatatca actccaaata agtattctat ataataatca ttcacattat ctatttaatt
    22861 atatcataac agaaaagctt agttataatt taataatatt attttaaaac aaaacttaat
    22921 aaaaagtata tctacttagt tttaatttga tagatatact ttcttcattt tacaaaaatc
    22981 ctctattaaa taagcaatta caatgccata ttttatatga aaataatttt taaaattttt
    23041 aatttaagtt cttatta
//
LOCUS       DAELSY010000058        23037 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.058, whole genome shotgun sequence.
ACCESSION   DAELSY010000058 DAELSY010000000
VERSION     DAELSY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23037
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.058"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(150..1079)
                     /locus_tag="KPG09_001916"
     CDS             complement(150..1079)
                     /locus_tag="KPG09_001916"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBG0966526.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTIYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(1094..1534)
                     /gene="def"
                     /locus_tag="KPG09_001917"
     CDS             complement(1094..1534)
                     /gene="def"
                     /locus_tag="KPG09_001917"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG0966527.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(1552..4077)
                     /gene="priA"
                     /locus_tag="KPG09_001918"
     CDS             complement(1552..4077)
                     /gene="priA"
                     /locus_tag="KPG09_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBG0966528.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNEIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(4316..5515)
                     /gene="coaBC"
                     /locus_tag="KPG09_001919"
     CDS             complement(4316..5515)
                     /gene="coaBC"
                     /locus_tag="KPG09_001919"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBG0966529.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLAFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(5520..5786)
                     /locus_tag="KPG09_001920"
     CDS             complement(5520..5786)
                     /locus_tag="KPG09_001920"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBG0966530.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(5789..6406)
                     /gene="gmk"
                     /locus_tag="KPG09_001921"
     CDS             complement(5789..6406)
                     /gene="gmk"
                     /locus_tag="KPG09_001921"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBG0966531.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(6436..7317)
                     /locus_tag="KPG09_001922"
     CDS             complement(6436..7317)
                     /locus_tag="KPG09_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBG0966532.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(7321..8151)
                     /locus_tag="KPG09_001923"
     CDS             complement(7321..8151)
                     /locus_tag="KPG09_001923"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBG0966533.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKINLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGVLEV
                     "
     gene            complement(8438..9472)
                     /gene="ltaE"
                     /locus_tag="KPG09_001924"
     CDS             complement(8438..9472)
                     /gene="ltaE"
                     /locus_tag="KPG09_001924"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBG0966534.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            9768..11543
                     /locus_tag="KPG09_001925"
     CDS             9768..11543
                     /locus_tag="KPG09_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBG0966535.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEIANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIVLSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(11758..13410)
                     /locus_tag="KPG09_001926"
     CDS             complement(11758..13410)
                     /locus_tag="KPG09_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG0966536.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEYLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGLLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLLEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGCFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYIISRNKKTPSRDKQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQITKLKNSF"
     gene            complement(13709..14920)
                     /locus_tag="KPG09_001927"
     CDS             complement(13709..14920)
                     /locus_tag="KPG09_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBG0966537.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKIVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLLLRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISIGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(15204..15737)
                     /gene="pyrR"
                     /locus_tag="KPG09_001928"
     CDS             complement(15204..15737)
                     /gene="pyrR"
                     /locus_tag="KPG09_001928"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBG0966538.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(16096..17043)
                     /locus_tag="KPG09_001929"
     CDS             complement(16096..17043)
                     /locus_tag="KPG09_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG0966539.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKAKYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDFVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEAIKKIK"
     gene            complement(17012..17458)
                     /gene="lspA"
                     /locus_tag="KPG09_001930"
     CDS             complement(17012..17458)
                     /gene="lspA"
                     /locus_tag="KPG09_001930"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG0966540.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRVIWEYVFNVADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(17768..18547)
                     /locus_tag="KPG09_001931"
     CDS             complement(17768..18547)
                     /locus_tag="KPG09_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0966541.1"
                     /translation="MKKAIWGICICLLSIVGVYTYNHNTHNQNSSKETYTLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQILDILKKNNVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYGSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDTIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(18597..19079)
                     /locus_tag="KPG09_001932"
     CDS             complement(18597..19079)
                     /locus_tag="KPG09_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG0966542.1"
                     /translation="MDTKKYKDKLLKEREKLTNLVVDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDSALSKIENGTYGFCDLCHNQI
                     DLERLDILPDTNLCNNCAKKENALLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGTLRD
                     "
     gene            complement(19263..20684)
                     /locus_tag="KPG09_001933"
     CDS             complement(19263..20684)
                     /locus_tag="KPG09_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG0966543.1"
                     /translation="MVSFLGSIVVLIVGYFIYGTFVEKVFGINDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGIVVAI
                     ILGLLFFKKAKDIKEQNAHKLAS"
     gene            complement(20830..21600)
                     /locus_tag="KPG09_001934"
     CDS             complement(20830..21600)
                     /locus_tag="KPG09_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0966544.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSSIEVVNEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYENYAVDAFSLDVFDYVLKP
                     YSEERIISMLNKLEKSEMSDIELSNSNSNLYKYKKETVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(21605..22807)
                     /locus_tag="KPG09_001935"
     CDS             complement(21605..22807)
                     /locus_tag="KPG09_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG0966545.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIVGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRTDNGT
                     LIVFYVKE"
BASE COUNT         7461 a         4129 c         2324 g         9123 t
ORIGIN      
        1 agtgatatta ttataatatt tatcatcaag gagaatattt gagttaaact tattataaaa
       61 aaattaacaa ctcctatcaa aattcctaat attaaaatca aaatcccaac acttgttaaa
      121 tacttcttta tatctgtcat acttttcacc taacccaata ctaaaccagt ttctatagta
      181 ttccctttta tatattcacc aaccaacatt ctctttttat tcggcatctg aatttcttca
      241 attaaaagta cattatctat agtacttact tttatcccat ctttgcttac atcaattatt
      301 gtacctacat cttttgtgct tttttcatct aacactttag ttttccaaac tttcattatg
      361 acatcattat agatagtata agcacttggc catggattta ctcctctaac taaattgtgt
      421 atttctctgg cactttttga aaaatctatg ttacctaatg atttattcat tattggcgca
      481 taggaaaatt cttcatgatt ttgtacttct cttggagcat ttccttcttc aattaatctt
      541 aatgtttctt ttaaagtttc tgctcctata ttcatcattt tatcatgaag ttctcctgct
      601 gttatatttt catcaagatt tacttctgtc tttagtatca tatctccagt atctaaccct
      661 tcatccatat acatagttgt aacaccagtc ttttcttcac catttattat tacccaattt
      721 ataggtgccg cacctctata ttttggaagt aaagaaacat gaacatttat acatccaagt
      781 ttaggaatct ctaatatttc ttttggaagt atctgtccaa aagctacaac tactattaca
      841 tctggattta aagatttaat tttatctata aattctttat ctctagcctt tactggttga
      901 tatacaggta tattattttc aattgccagt tcttttactg gactcatacc tagtttttta
      961 cctcttcctt ttggtttatc tggttgagtt acaacaccca atatttcata cttctcatct
     1021 attatttttt gtaaacatgg aactgctata tcaggagttc ccataaatac tattttcatc
     1081 aaatcaactc ctactacttt tctattttgt caacaaataa aattccatct aagtggtcta
     1141 tctcatgaca aaatgctcta gctaaaagtt cttctccctc taactctatt gtttcgccat
     1201 ttctatttaa agctctgacc tttacataat ttggtcttct aacatttcca gcttctccaa
     1261 caacacttaa acatccttcc tcatctattt gctccccaga tgtttctatt atttcaggat
     1321 ttataagttc tataagctct tctccaatat ctattactac tactcttttt aatattccaa
     1381 cttgaggtgc agccagtcca acaccatctg catcatacat agtttctgcc atatcatcta
     1441 ataattgtat tatcttttca tcaatttttt caaccttttt tgattttttt cttaatactg
     1501 gttctcctat ttgaactatt tgtcttaaag ccattataat tcctcctaaa tttataatac
     1561 actgtttgga ttaatatcta tagatacatt gatattctta tcaaatatca aatctcgttt
     1621 tgttatacat atatatttta ttataccctt taagagatta atttcaatat tttcatcttt
     1681 aaagagtatt tgccatctat aattttgatt tatttttgat attgaacatg gatttggacc
     1741 taatataaat cctaaatcat ttatacccct tttttctaat aaataaatca atgatgcatg
     1801 catatttttt atatttttta tcactaatct ttcatcttca ccactaacaa ctacacttaa
     1861 catattatta aaaggagaat atccaaatgc ttttcttatt tttatctcat cttcataaaa
     1921 accctcataa tcatattcaa tagcatgctt aatagcatag tgttcagtgt cataagtctg
     1981 aagtactact tttccctctt tatcagctct ccctgctctt ccagagactt gagttatcaa
     2041 ttgaaatgta gtttcaaaac ttttaaagtc tggaaaattt aatatcatgt ctgcagacaa
     2101 aatacctact aaagttacat tctcaaaatc cagcccttta cttaacatct gtgttccaat
     2161 aagtacatct gcttctttat ctttaaattt atttagtatc tcatctaaag cacctttttt
     2221 tgaagttgta tctttatcca ttctcaaagt ttttatatct ggaaaaatat attttaattc
     2281 ctcttcaatt ttttgagttc caactccaaa tggcttaaca taagtacttt tacattcagg
     2341 acactctttt ggaatttctt tctcatatcc acaatagtgg catcttccag tattgctttt
     2401 cttatgataa gttaaagata tatcacaatt ttcacattga aacacatatc cacactttct
     2461 acaagataca aaatttgcat aacctcttct gtttaaaaat agtatcactt gattattttc
     2521 ttcaatcgca taacgtattt ctttttgtaa tttcaaacta aatatacttc tatttccttt
     2581 atctaattca tctttcatgt ctaccacttc aatgtttgga agaggcttat catttgctct
     2641 actttttata tttataagtt tgtattctcc agattttgcc ctataatact cttcaattga
     2701 gggcgttgct gaacctaaaa taagagttat attatttttt aatgccataa atctagcaac
     2761 ttctatagca ttaaattttg gattcttctc agacttatat gatgactcat gacattcatc
     2821 aattattatt aagcctaagg aattaaaagg tgcaaataaa gctgacctag ctccaattaa
     2881 cactcttact ttacctgctt tgacagcttt gtaaacatca tgtttttctc cttcagatag
     2941 tttactgtgg aacactccaa ctaaatctcc aaacctactc tttagcctat ctattgtttg
     3001 aggagttaat gctatctcag gtactaagaa tatactgtct aagccttgat ttagagcaaa
     3061 ttctattatt tccatgtaaa cttcagtttt tccacttcca gttactccat gaattaagta
     3121 tggtttttta tcatctacaa acatattcga ctttatctca tcaactgctt cttgttgttc
     3181 attgtttaat tttatacttt ttagtacgga tttgtaaata ctttttggct ctctataata
     3241 atccttaaat tctagggtta ttagcttttt cttacttaga gaatttatac tttgttttga
     3301 agcatttaat aaatccaata aatcatttat ctctacattt tcattatttt taagaaaact
     3361 taatatctct ttttgtttac ttcccacatt tattttattt tgctctatat aatcgtcaat
     3421 cttatcactt tctaaactca aagatatata acatactttc ttttcatttt tgtgattttt
     3481 atattcccaa cataatttta taattccatt tttattcatt gtgtacaaag aactatttaa
     3541 gtttggtata tattttaact tatcaacctt aattttacct ttactatcaa tcactttctc
     3601 tactatttct ctatttttat cacttaaagt tgatattttt tccttcaatt caatctcatt
     3661 taatccactt aaactttcac ctaataggac tactttataa ttatttaatt tgtatccctt
     3721 tggatatatc aaatttatac attctatata agtacacaaa tatctatttt tcatccaata
     3781 aaccaactct aaatcttctt ttctaaatat tggattttca tctaatatat caataatttc
     3841 ttttattttt attttttcat ctaagctatc tgtaaattga aatacaaagg cttctgttgg
     3901 tttattacct tttccaaatg gtactaatat tctgtgcccg atttttatta catcagataa
     3961 aaatacagga atttgatatg taaataaatt atctgtatat atagtattac ttctaactat
     4021 tacctttgca tattttttca ttataaaacc tctacttaaa tttaatatat tgttcatatt
     4081 ttaatatact ttacaagata ttaaaatctc agaatttata agcaatttca agttattaat
     4141 attaacttta ttttaataat taactattaa ttatatttta tactttatga caaaataaat
     4201 gctgaaaact acaatttaac tgacatttgc gagtttttac tcgctaaaat taaattgcag
     4261 ctttcagcca ctttagctgt gataaaatac acaaatatca tatttaaaaa aggtcttatt
     4321 tctcaagcaa cgctttaact ttatctaaaa taacattggc tacttcttct tttttcatct
     4381 taggatactc agtaatattt ccctctacat ctattatttt tacaatatta gtatcagctc
     4441 taaaaccagc accttcttta gttaaatcat ttgccacaat aaaatctaaa tttttctttt
     4501 ttatttttcc tttggcatgt tctataaggt cattagtttc agctgcaaat ccaattagta
     4561 tcttatcttc ttttattttt cctatttcat aagcaatatc ataatttcta cttagttcta
     4621 ttgataaatc atcattcttc tttttaattt ttttgtcaga ataagttttt ggtttataat
     4681 ctgcaactgc tgcactttga attactattt tattttcact taaatttgat actactgctt
     4741 catacaaatc ttgtgctgac tctatttgaa caaatttttt aagattttgt ggaatagaaa
     4801 gatttgtagg acctgatatt aatgttacat cagcacctct agctatagct tctcttgcta
     4861 tagaatatcc cattttacca gaagacctat tagttaaata tcttactggg tctatactct
     4921 ctacagttgg accagctgta actatcatac tttgaccttt taaatcttgc tctttagtaa
     4981 gattagtcaa aacttcctct actattatgt ctggattagc taatttgcct gttccaatat
     5041 ctccacatgc aagcctccca ctttctggtt caataaattt atatccaaag ctctttaaag
     5101 tttgtatatt tttttgaaca attggatttt catacatatt agtattcata gctggtgcta
     5161 taagaacctg tgctttggtt gccataacag tagttgtcaa catatcgtca gctataccat
     5221 ttgcaatctt gcctataaca tttgcacttg ctggaactat taagaataaa tctgcttttt
     5281 ttgctaatga tatatgttca acttcccaag tttttggttc ttcaaacata tcttctacta
     5341 cataattttg actcagcgat tgaaatgcaa gtggtttaac aaattctgtt gctgattttg
     5401 tcattataac atggatattt gcatttagtt tttttaactt acttacaaca tcacaagcct
     5461 tatatgcagc tataccgcca cttatgccaa ttacaacagt tttatctttt agcatgacct
     5521 tactcctctt ctgaaatttg ttggtgttgc tcagcatgat gtctagcttc ttctatctct
     5581 atttcctctt ctgtcaataa tctatatgtt atttttccac ttgcaacttc tttagttgct
     5641 acacatacag gcttttcctt agttttattt gaaacatatg gttcttctcc atcaattaat
     5701 tttcttgctc tctttgaaac agtccctact aaatagtatc tgttgtctat tttttctagt
     5761 acctcattga tagatggttt taacataatt ataattcctc cttaaatttc tctattataa
     5821 tatttttata tctgctaact ttattctttt cagatgatat tataccttct atttctttag
     5881 cagatgtttt tacatcttca ttaaatataa aataatcata atccttaatc attttaattt
     5941 cttcatatgc acagctaaat ctcttctcta tttcagcttc tgtttctgta cctcttccaa
     6001 caattctatt ttttaattct tcaagtgatg gtggtagtac aaatataaat actccctctg
     6061 ggcaaacttc ttttatttgt tttgctcctt gcatttcaat ctccagtaat acatcttgtc
     6121 ctttttctaa acattccatt attgccgctt ttggagttcc ataaaaatta tcataaattt
     6181 gagcatactc caaaaactca cctttttcta tcatctcttc aaacttttcc ttacttatga
     6241 agaaataatt tactccatca acttctccag ttctgggttt tcttgtagta gctgatacag
     6301 aaagttttat tgtatcattt tctttaagta gttctttgca aatagtacct tttcctgcac
     6361 ctgatggtcc tgatacaact agcaataatc cttttttcac aaacatttaa gcattcccct
     6421 tctaatattg tattcttact ctatattttg tatttgttct cttatttttt ccaattcgct
     6481 ttttacttct actactaaat tagttatatt tatatcagat gacttcgaac ctatagtgtt
     6541 agtttctcta ttcatctctt gtattaagaa atctattttt ctacctatag agtcgtcttt
     6601 aaatatagta tttttaagtt gccctatatg acttttaaat ctaactattt cttctgttat
     6661 actgctttta tctgcatata ttgcaacttc ttgtgctaat ctattctcat ctattatact
     6721 tgggtcatcc agtaattcac ttattctcaa attcaatttt tctctataat cttctacaac
     6781 actacttgaa tatttttcaa tctcttctat atgatttttt aataaatcac atctcatggc
     6841 aatatcttca gctaattttt taccttcttc acttctcatt tctcttaatt tgattaatgc
     6901 atcttcaact gcttgattca acattgacca caataaatct tcatcttctt ccttttcttc
     6961 tattttcaca atatcaggaa actttgcaac attcataacg ctaatatcat ctataatatc
     7021 aaatttattt tttatttctt ttagtatgtc tatatactga gatgctaatc cctcatcaaa
     7081 atttaagttt acatcttctt tgcctaataa gtcaaacttt atgtataaat ctactcttcc
     7141 tctttttaca taatttttta ctaaatttct aactttatcc tctaaaaatg aaatttttct
     7201 tggaagacgt atgtttatat ctgaatactt atgatttatc gttttacact ctactaaaaa
     7261 gtaataatta tcatccttgt attctcctct tccaaaacca gtcatactta tagccattaa
     7321 ttaaacctcc aaaacacctt cacatatttt tactgctgga cctgtcatat aagagaaatc
     7381 tcctacatct attttaactg ttccaccttg agacgttaca tttacactac tttctaccat
     7441 attaagtaaa ttgcacacta ttgctgatgc agtcatacca gtaccacaac ctaaagtata
     7501 tccacaacct ctttcccaag tataaacatg tatattatac ttatcttcta cctttacaaa
     7561 atttacattc gttttttcag gaaatatttt attattttca atctcttttc catatttaca
     7621 aacatcatct acatctaatt catctactat aactactgta tgaggaaccc ccatcaacat
     7681 agaagtaact ttaagctccc tatctaaaac ctttatactt tcttgcacaa aacgttcttt
     7741 atttgtaagt acaggaacat ccttagctat aaaacttcct tttcccatat tgactcttat
     7801 agagtttatt tctctattgg taagatttat ttttatatct tttataccat ctaatgtatc
     7861 tactgtaaaa ctttcttttt taactataca attatcataa acaaatttac aaaaacatct
     7921 aaggccattt ccacacatag ctgctcttga accatcagaa ttataatata ccatttctac
     7981 atctgcaact tttgaatctt tcacaactaa caatccatcc gctcctacag aaaatcttct
     8041 atggcatact ttttttgcta aagcagtata atcagatgga tttatctggt cgaatcttcc
     8101 atctattgct ataaaatcat ttcctattcc atgtagtttc caaaatttca taagtatcgc
     8161 tccttcgtaa attaacgctt aaactattat aacataaaaa aattcccata aacttataat
     8221 ttatgggaaa agttttgtaa tgacaactat aatttgagga tgtgagggag agttgtcatt
     8281 acaattaggg taattatatt atgtttttaa gctagttctt tctatgttta tatattaata
     8341 tttctatatg aattttatgt atgttttatt taaactgtct atgaattttt taattaaaca
     8401 tctggtaacc agctataatc cttttagtag atatctatta acatacacct attaattctt
     8461 taagtgtttt taaaacatag tctaaatctt ccttagtaat atctacatga gttacaaatc
     8521 tatattcacc attttccatt ttattaattt ttatatttct ttcaaaaaat tcttttatta
     8581 aaacatcctc ttttataata tcttcactca tcttaaaaaa taccatactt atatctggat
     8641 taacattgtt tacttcaatg ccatcaattt tatttaattc acaagctaaa tattttgcat
     8701 tttcatggtc ttcattcaat cttaaagtca ttttttcaat ggcaattata ccagctgctg
     8761 caactatacc tacttgtctc attcccccac ccataacttt ttgctttctt ctagcttttg
     8821 ctataaattc tttacttcca gcaagtatag aacccattgg tgcagctaaa ccttttgaca
     8881 cacagaacat aacactatca caacactttg ctatttcttt agcttctaca cccaaagtta
     8941 atgctgcgtt aaataatctt gctccatcta aatgtactgg taagttgtgt ttctcagcta
     9001 tttttttaat ttctcttaaa ttatcaagac tcacaacagt tccacctgaa tatgcatttt
     9061 ctacacatat aagccctgtc tgtggttcat gtatgtcatc tcttctaatt gccttttcaa
     9121 caagttcagg atttagagct ccactttcgt cttcatctat agttctaagc tgaactccag
     9181 ctattacagc aggtgcccca acctcatgag ttacaatgtg ataaccttta ccaataatta
     9241 tttcttgccc tcttaggcaa tgagtaaata gagctaattg attcccaaaa gttccacttg
     9301 gaaggaaaat agcatcttct tttccaactt tagctgctgc taattcttca agtttattta
     9361 cagtagggtc atctctaaag attgaatctc caacaggagc ctttgccata gcttctctca
     9421 tttcgtcagt aggcatagta acagtatcac ttcttaaatc gataaatctc attttttcaa
     9481 ccttctttca tatttatata ctatagaacc ttaaaattga ttttttctgt aaagttcata
     9541 tactttcata gcttatactc tacacaataa ttatactata tttactttat atttaatcta
     9601 ctttaaatta ttaattttaa aaataaagtt tcctctctaa taagtataaa taattataca
     9661 atttaaatat taatctctat cgtttaaatt ccccctagtc acaaagcgta tattatgtgt
     9721 ataattaatt tactgactca ttttggtcaa agaaaggggt gctataaatg gcattagatg
     9781 gattagttat acattcaata gtcgatgaat tatcatcaaa acttacaggt ggaaaaatag
     9841 ataagataca tcaacctgaa gatgatgagg taatttttaa tataagaaat aataaagaaa
     9901 attttagatt ggttttaagt gcaagtgcct caaatcctag agtttatctt acaagtaatt
     9961 atcaaaaaga aaaccctcta aaagcgccta tgttttgtat gttacttaga aaatacatac
    10021 aaggtggaaa tatagtagaa atttctcaaa taggttttga aagaataata aaaataagtg
    10081 ttgaatcttt agatgaatta aaagaaaaga cagttaaaaa tattatgatt gaaattatgg
    10141 gaagacatag caacataata ataactcatg gtgaagaaaa taaaattata gattctataa
    10201 aaagagtccc ttttagcata agtagagttc gtcaagtttt acctggtcat gattattcat
    10261 taccaccaga acaaaacaaa ctcaaccctc ttgatgatat atctaaggat ttgtttatta
    10321 aaaacctaga agaattggaa ggacctatct ttaagtctat atattctaaa tttctaggta
    10381 taagccctat tatagctaaa gaaatttgct acagagcagg tgtaaatcaa aatgctatca
    10441 taaaggatat atctgatgaa caatttgatt ctttacacaa agtattttgt aatttattca
    10501 atgatataaa ttctaacaaa tactcccctt gcattattat agataaaaag gtggataagg
    10561 tagttgattt cagttgtata aaccttacat tatttagcga tttatcatat ataaataagg
    10621 atagtatgtc tcgtattcta gaagatttct atagaactaa agatattaaa gacagaatta
    10681 atcaacgttc atctgattta aagaagagta tatctgttaa gcttgataga ctttataata
    10741 aacttaaaaa acaagaagaa gaattatcag aatccgaaaa tgcagacatt tataagataa
    10801 aaggagaatt gattacttca tatatttata tggtagaaaa aggtatggaa agtattgaaa
    10861 ttgctaactt ctatgatgaa aattgtaatg atgtaacaat agaattaaat aaaaacttga
    10921 caccatctga aaatgctcaa aaatacttta aaaagtataa caaaatgaaa catgctaaag
    10981 tcgagatatc tcatcaaatt tccttaaata aggaagaaat agattactta gaaaacatag
    11041 ttttaagtat tgaaaactgc gaaaatttag ctgaattaca agatataaaa gaagaacttg
    11101 ctaaggttgg atatatcaag actcaaaaga aaaacagtaa aaaggatact atcccatcta
    11161 caaaacctca tgaatttgtg tcttctgatg gatttaaaat attggttggt aaaaacaata
    11221 agcaaaatga ctatcttaca ctaagacttg ctgataatga tgacttatgg atgcatacaa
    11281 agaatattcc tggttctcat gtaataataa agtgtgctgg taaggaagtc cctgataata
    11341 ctgtatttga aggagctatg ctagcagcat tctttagtaa gtcaaaactt tcttctcagg
    11401 tgcctgtaga ctatactaag agaaaaaatg tgaaaaaacc tagtggttct aaaccaggta
    11461 tggttattta tgaaactaac agtactattt atgttactcc tgaagaagaa acagtagcca
    11521 agcttaaggt taaatctgaa taaattctgc actaaataca aaataaatgt tagacaagca
    11581 cacattatca ttctaaataa aaatagtggc cttcttataa agtacaataa taaggccact
    11641 ttctaattag cttactttta attttctaac aaaatacttt tctgttttaa taactatact
    11701 tatattccca acaaatattt tcagtcttct tatatagatg taattaaaaa gttaacacta
    11761 aaagctattt tttaatttag tgatttgttt tagtaattca ttatattctg attcatacaa
    11821 ttcttttttt agattatctt tttctataga taacatagat ataacagttg taagtctatt
    11881 ttctaatact agaagattct cttttttgat ttgcttgtct ctactaggag tttttttatt
    11941 tctgctaatt atatattcgt cataattaca atcaaactcc cttattttag catcttttat
    12001 ctctataata tagttacaaa tatgtgatac aaatactcta tcatgagaca ctataagcat
    12061 agttttatca gtatttatca gtgccttttc cagtgcttcc atagatttta tatctaagta
    12121 atttgttggt tcatcaaata ctaaaaaatt attatcttcc aatattattt tacatagtgc
    12181 tatttttacc ttttctccac cactaagtac ttttactttt ttgtagacat catcaccttt
    12241 aaacccaaat aggcttaagt ttatccttat aaaagattca tcaaatgaac tattaacttt
    12301 agtattgtat agaacactct tttcctcatc taataaactt tgattttggt caaaacaacc
    12361 tacttttacc ttgttattta tttttatatt gtcattttta tcagccaata tttcttttat
    12421 cagagtactt tttccacacc cattatcacc taataatgct atctttttac ctctttttat
    12481 cttaaaggaa acattatcta acaagagctt attttctaac tttagagtca tatcttttac
    12541 ctctaataga tttttgctaa ttatctccat accatcttga atcttaatgt tcatttcttt
    12601 agaaacttta ggtttattct ttacctcaag tttatcaatt ctgcttttta tagccttcac
    12661 atttgagtcc aactttttct tattagtctg accacccatt ttgtgcaacc tagcttctga
    12721 attacccatt ctttttggtg tctttcttat gccatcactt aaagcttttt tatataaaag
    12781 agctttttca agcctttttt tctctttaac atattcttga tactcaaaat cagctctttt
    12841 tacttcttca tctttttgca ttaaataatc agtgtaattg cctttgtaag cttttagttt
    12901 tccatcttct agttctaata tagttgtaca taactcatcc agaaaacgtc tatcatgaga
    12961 tattaataat aatgctccct catgcctttt aaacatatct tccaaaagcc caatgctttt
    13021 tttatctaag tttgaagttg gctcatcagc tattatgata tttttcttat tactcagcgc
    13081 atctgctatt tttaacttaa ccttttcccc tcctgataag tattcttcaa atttatctgg
    13141 agcatttaaa agactcttct ccttactatg actacatgtt tcaatattat tttcactctg
    13201 acttatataa gaatagcttt ctgtaaggta agtatatcct tcatcaattt ctatttcacc
    13261 caatattgct tttaataatg ttgtttttcc ttgaccattt gaaccaacta gacctatttt
    13321 gtcattttct aatatttcaa gtttatctat atccaaaatt aatttatctg cataatattt
    13381 ctttaaattc tctactttta ctaacaacat aaaaaatccc tccttaacac acttaaatat
    13441 aaggagagat tctataaata ggcataacaa ataaacgtat agactataat ctactctcct
    13501 taatttaagc attgcaaata aagccatcaa aataggcttt tcaaaaatta caatacttta
    13561 aattataaaa aatagattat attctcattg tcctagttac cttgcccttt ctttcattta
    13621 tttttaatca ttatatcaac attaattaca aatgtctata gaaaattaac taattttagt
    13681 tattcaatat acaatttatt ctttaaattt attcttcttc aactttaaaa tcctgattat
    13741 ttaaaattct gtttaataca attccaacaa ttgcagctaa actaagacca gttatcttta
    13801 ctgtacttgt tatagggatt ccaatagaaa tgcctttatt agataaatat gttgttccaa
    13861 gacctattaa taatataact gctattatta atatatttat cttactttct acacaagaac
    13921 tgtcccttat agtttttaat cctacataag ttatcattga aaatagcatt atactaattc
    13981 cacccataac tgaacctggt atactttgaa gaaatccacc aaactttcct acacatgcaa
    14041 gcaatatagc aaatattgct gtacgtctaa gtattgaagg gtcatagttt ttagttatag
    14101 caagtactgc tgtattttct ccataagttg tattggctgg tcctcctaaa caccctgcaa
    14161 ctatagtcgc aaatccatcc ccaagtaatg ttctatttaa tcctggattt tctataaagt
    14221 ttttacctac tacagcacca ttagtagtca tatctcctat atgttccata aatacagcta
    14281 atacaactgg agatataatc acaattgctc ctaaactgaa cttaggaact gtaaagttcg
    14341 gtattgcaaa taaacttgct tctgtaatag tagttatatc tactaacctt aacagtaaac
    14401 agattatata cccagaaaaa acggctatta atattcctaa ttgttttata aatcctctac
    14461 caaacttatt ggttaaaatt gctattgcta aagttataaa tgctattaca aaattagctg
    14521 aagccatact aaaagctgtt ggcaataaat ttaatcctat aactactatc atagctcctg
    14581 taacttgtgc aggaaaatat aatttaattt tatttacacc aactattttt actataatcg
    14641 acattataat ataaattaat cctgctacta ttataccacc ttgagcatat gctaaatctc
    14701 caccatacgc ctctttagct gctagtataa ctggtataaa cgcaaaactt gaacctaaaa
    14761 aggcaggtac ttttccttcc gtacaaaaat gaaatatcaa tgtaccaaca ccagcacaga
    14821 aaattgcaac tgacggatta aaacctgtca aaatgggaac taatactgtc gctccaaaca
    14881 tggctaatag atgttgtaaa gacattattg tttttttcat aaaaacctcc aaaattatta
    14941 tatataaata aatcctctgt ttaattaata tcttttaatt aattatcatt ccctagattc
    15001 tattttttaa cctctatata cttattatat aatttatcat tattttacat tttacgacat
    15061 caaaaactca aataaaggag aataaaaact acattaagac tcacatccat aaaattataa
    15121 tatttatttt gtgcaatcta tgtaagcttt atcctcctcc ctgactattc aatgctaagc
    15181 ttttatataa tacttaaaac taattattct ttgattgtaa ctgaatcttc tccatcaatt
    15241 tctaataaac tgacagatat aatttcatgt ctagaagtag gtacattttt tcctacataa
    15301 tctgctctta taggcaactc tctatgccct ctgtccacta gaactgctaa ttgtattgat
    15361 ttaggtcttc ctatatctat aatcgcatct agtgaagctc tcacagttct tccagtgtat
    15421 aaaacatcat caaccaaaac tactgtctta tcatttacat taaaatctaa ataagttcct
    15481 ttaactacag gttcaacatg aattttttcc aaatcatctc tatataaagt tatatcaata
    15541 tctcctgtat ctacttttgt tccttctatt tgttctattt tcttagaaat tctatttgca
    15601 attggcacac ctctagtttt tattccaact aaaactagat tttctacacc tttatttctt
    15661 tcaattattt catgacttat tctagtaatt gctcttgcta ttgccttttc atccattaat
    15721 tgagcttttt ctaccatatc ataattattg tcaaagatat aatatatcta tttgacaaat
    15781 ctactccttt cttttaattt tttataaatt tatttttatt taaaatttca atttaaacaa
    15841 caaaaaactt ctcatcacaa gacaagaagc tcttatataa aaaatataca gtgtaattta
    15901 gcaattgtct tacgatatct ctgtatcgtt taaagataat tttttaatct atcaactcaa
    15961 attatatatt tatatttcaa atttttcaag cttttttaaa caaaatactt taatataaga
    16021 tattttattt aaatttcact aaatttattt acttatataa aaacaaaaaa tgatttataa
    16081 gtattataca aatggttatt ttatcttctt aatagcttcc ttaaaatact caggtaattc
    16141 tgaattaaat tcgatatatt ccttagtggt aggatgtata aatcccaact tcttagcgtg
    16201 caaagtttgt ccttttaatt taaatttagt ttcttttgga ccatatacaa aatcacctaa
    16261 aagtgggtga tttatagaca aagcatgaac tcttatttga tgagttctac cagtttcaag
    16321 cctagctctc atatgtgtaa acttatcaaa tctttctaca acttcaaaat gagtaattgc
    16381 attttttcca tcctttacta cagccatttt taatctatcc ttaggatttc tccctatagg
    16441 tttatcaaca gttattttat cttctttaac cacaccatag catataaatt catactctct
    16501 agttatagta tgttccttta actgttctgc tagaaaatta tgtgcataat tgttcttagc
    16561 tatcataaga agaccagaag tatctttgtc tattctatga actataccag gtctaataac
    16621 tccatttatt gacgacaact tatctttaca atgatataaa atcccattta ctagtgtatt
    16681 ttcatagttt ccaggagcgg gatgaacaac catatcctgg ggtttattta ttattaagac
    16741 atcatcatct tcatatacaa tatctattgg aatatcttga ggtaaaacct ctaatacttt
    16801 aggttctggt atctctataa caatattatc ttcctcttta actagatatt tagctttttc
    16861 tactttatta ttgaccttta cttttccatc ttttataatt ttttgtatat aacttcttga
    16921 catatcacct aattgttctg ctagataaat atcaagtcta acttcttctt cctcttcaag
    16981 tactaaaaac tcttttactt catccattat atcacctact tttactttca aaaaataaaa
    17041 catatataca taaaaacaca gttcctacaa ctacaaatac atcagctaca ttgaatacat
    17101 attcccatat aactctaaag tcgaagtaat ctaccacaaa acctaatcgt actctatcaa
    17161 ttagatttcc taatgcacca gatataatta atataattcc caaccttcca aatatatgta
    17221 ctttccttgt attaagatag tataatccaa atactgttgc aagtaatgca acaattataa
    17281 atatccactg attattttgt aataatccaa atgctgcacc tctattttcg acataagtta
    17341 aatgaaatac attatttatt attggtattg ttgaaacatc taccaaatta ttcaataccc
    17401 atattttaga cagttggtct aagcctatga gtagaattat tattaatata tatagcaatg
    17461 atttttcccc ctataaatta tttaaaaacc aattttattt gaaacacact attattatta
    17521 tacaaaaaaa atgctctaaa gagcaacttt attaaaattg tattttctta ataaagctag
    17581 agcaaaattt aaaatagtac catctttttt atattgcaga ttgctacata tttaaaatca
    17641 tattattatg tataaaaaat ttaaaaataa catactaatt aatacttttt gtgtgtttta
    17701 tagattaaag attaatgatg tctgtacttt aaacttacaa gcaccactaa tcttcaaaca
    17761 aatattttta cttttgcact tgcttatttt tatctaaatg ttgtataagt tcagatactg
    17821 ttacgaattt atatcctctc tcttttaatt taggaattac tacttctaaa gcttgtatag
    17881 tttgtgattt atcatttctt ttagtgttat aatcatgtaa aagtattata ttaccatttt
    17941 ggacctttgt aagaatagta tttataattg tatctacacc agggttagac caatctctag
    18001 ggtctaagtt tgaccaaaga attatattca tgcctttaga aactataata tcacagacac
    18061 ttttactcat ggctctatat ggaggtctaa atatctttgg ttctactcct attacacttt
    18121 ttataatatt ttgtgtatct gttatttctt tttcaacact accataacta ttttttgcaa
    18181 catttatatg tgtaaatgta tgattcccta tttcatgccc ctcatcatat tgccttttta
    18241 ataattcttt gttatattct actttttctc caactacaaa aaaagttgct tttacattat
    18301 ttttctttaa tatatcaagt atttgtggtg taaaatcctc atctggacca tcatcaaaag
    18361 tcaatgctag aatcttttca tcctcatttc ctttttttat tattacatct tcataatcgt
    18421 cttttatatt gtcatttaaa gtataggttt cttttgaact attttgatta tgtgtattat
    18481 ggttatatgt atatacacct actatggata ataaacatat acatataccc catattgctt
    18541 tcttcatgtc attacctctt ttctaattca ttctaacaat atatgatgtc aggcttctaa
    18601 tctctcaatg ttccattttt atttaaatca gttaaatttg ttaggtcttc tgaataaaag
    18661 ttagaatctt ctacatcagg attttgcatt gcatcaccta ataaagcatt ttcttttttt
    18721 gcacaattgt tacataaatt tgtatcaggt aatatatcta atctttctaa atctatttga
    18781 ttgtgacata aatcacaaaa cccatatgtt ccattttcta ttttagataa tgctgaatct
    18841 atttgataca gtcttccttc ttcatgattt attaaactat tttgcatatt ttgcatatat
    18901 aaatccgttc ccatatctcc aatatgattg tcataactag ataattctcc tgatgaatat
    18961 ttttcactag tatgtttagt agtgtctcca aacaaggtat tatctttcat atctacgact
    19021 aaattagtta acttttctct ttcttttaat aatttatctt tgtatttttt agtatccatt
    19081 ttcaacacct ctaattttaa tatttatatc ataaaaatag tatgtggaaa actaaattaa
    19141 aaatacaata aaaaacaaga tttgattttt tcaaaaaaaa ttgtaggtaa acttaaaatt
    19201 cacctacaaa ttctttacat atatttttaa tataatgttt ttagtttttg ataataatta
    19261 cattaacttg ctaatttatg agcattttgc tcttttatat ccttagcttt tttaaaaaat
    19321 aaaagtccaa ggattattgc aactacaata cctattccat tagatatatt aactggaagt
    19381 ctaaatcctt cttttgcctg cattatataa ctaaatgtta ctacagtcat aaaaagtgct
    19441 ggcaatgtag ttatgtaatg atttttattt gctttgacta aatatgcaga accagtccac
    19501 aacattatca tagcaagtgt ttgatttgcc caagagaaat atctccaaat tatattaaaa
    19561 tctataaatg taagggcaac ccctacagca aataatggca aagctattaa aaatctattt
    19621 ttaactgcat cttgattata attcattgca tcagctattg ttaatctagc acttctaaat
    19681 gctgtatccc ctgaagttat aggacatgct acaacaccaa gtatggccaa agcgcctcca
    19741 ataggtccca ttaatccaac agatactgta tttacaacag ttgctgctcc accatcatta
    19801 aatattacat taagctgagg tataccatgg aaatatgaca tagctgctgc tgcccaaact
    19861 aaagctatta taccttctgc aaccattgac ccataaaata ctggtcttgc ctcactctct
    19921 ttctcaacac atctcgccat aagtggtgat tgagttgcat ggaaacctga tattgctcca
    19981 catgctattg ttataaataa gtatgggaat aaaggtgttc catttggatt catattttgt
    20041 aaatttatat ttggtatgtt ataccccttt attattaatc ctccagctac acctactgcc
    20101 ataattaata atgctgctcc aaataaagga tatattttac caatcaattt atctattggt
    20161 aaaacagtag ccataatata ataaagtatt atcactccaa cccatatttg tgcgtttaca
    20221 cctgtcaatg tctttaataa tcctgctgga cctgttacaa atacaactcc aaccaatact
    20281 aaaagaataa cactaaatat tctcattgct atttttgcac catttcctag ataaagacct
    20341 accaactctg atgcagatgt acctttgttc ttcattgata aatatcctga agcaaaatcg
    20401 tgtactccac ctgcaaagat tgttccaaac acaatccaca aaaatgctga tggtccaaat
    20461 aacgcccctt gtatagctcc aaatatcgga cctaaccctg ctatatttag aaactgaatt
    20521 aaaaagattc tcttccattt cattggcaca taatctactc catccctaca agctatagct
    20581 ggagtttggt ttttgtcatt gattccaaaa accttttcta caaaagtacc atagataaaa
    20641 taacctacta taagtactac tattgaacct aaaaagctta ccataaattc atccccttag
    20701 tttttatata attgctttat aaatttttat gagtttaaga aaactatttc cagttcaaat
    20761 agtaatactt ttttatgcta atataaatat taattacatt aagtgcatat ttaagtacat
    20821 gaaatgactt tatatatgca ttaacagcct aaattctttt actttgcttc tgcttacagt
    20881 tacttcatag tcactatttt ttaatttcaa tatgtatgta ttgttaaacc atggtataat
    20941 ttcttttact ttggttaaat ttacgatata agacctatgt gttctaaaaa atgttttatc
    21001 atttataaga ttctctactt ctgatatgcc ttcttttaat ataaactttt ctttctcagt
    21061 atatataaat gtctgccttt cattagcttc acaataatat atatcatcaa tatctattac
    21121 aaccaattta tcacctttcc aaaggctaat tttatgtgtt atttcttcta tctcctgatt
    21181 tactgtctct tttttatatt tatataaatt actattgcta ttagacagtt caatatcact
    21241 catctcactt ttttctaatt tattcaacat agaaataatt ctctcttcag aatatggctt
    21301 cagtacataa tcaaatacat ctaaagaaaa agcatctact gcataatttt catacgctgt
    21361 tataaaaaca attttaggcc ttgatttaaa ctgatttaat gtttttgcaa gaagcattcc
    21421 atcgagatga ggtatattta tatccaaaaa aattacatct attttatttt cctgaataaa
    21481 gtctaacact tctataccat tagtaaattc atttactacc tctatgctac ttttattctc
    21541 aataaaatat cttaattcct ttcttgccgg aaattcatct tccactatta tagctctcat
    21601 acttctactc ctttacataa aatactatca aagtcccatt gtctgttctt cttatattaa
    21661 gccctcttcc atacatcagt ttaagcctta aatgtacatt ataaagtcca atcttatttt
    21721 ctggcatatt atcttgatat acattattta taatttcctc acttatacct ataccattat
    21781 cctctattga tattctcaca gtattttcta atgaataagg taactttttt atggatattt
    21841 tcactacacc agctctacca ctttctaaaa taccatgtat tatcgcattt tccactaatg
    21901 gttgtataat caaacttgga atctttatat ttacatcttc tatatcataa gatacagtta
    21961 gtaattctcc aaacctagct ttttctatct ctacaaaaga tttaacttgt tcaatctctt
    22021 tatttatatc aattaagtta tcactatatt caagattata tcttagataa gttgataaat
    22081 ttattattaa ctctctagcc ttatctggac taaatcttat aaatgaagct attgtgttta
    22141 gggaattaaa caaaaaatgc ggattaattt gtctttgaag tgcttttatt tctgctttat
    22201 ttctcgcttc tatatctttt ttatctttct caatcccctc tactatagat ataacaaatc
    22261 ctattccaat ctgtccaaca attaaaggta aatatatccc aacaattatt tgaattgcta
    22321 aagaatgtgg atatgatatt aagtatataa gtagtatagt aaaactttca gaaatcatac
    22381 ctaccaatat accatacatt actctatgtt gctttggtat cctcttatat agaaatcctg
    22441 ataatacacc tgctagtata cttgataaaa gacatggaac agatgttatc cccccaatat
    22501 ccataaaata tctatgaatc cctgaaaaaa ctcctgctgt aatacctacc attggaccaa
    22561 ataaaatgcc acctgatact atagatatta ttctagtgtt tactattgac cccatataat
    22621 ttataccact ataagttcca aatatggcta aaaaagtaaa aactaatgct atacagacca
    22681 agtcattaaa actatattct tccttttgaa atatctgctt aaatattttt aactttgtta
    22741 ttagaagaaa cagtactaca atgaggcttg tcttttctat taatgcgatt aaaattgatg
    22801 ttttcatatt ccacctttaa aaaataaatt tttacacact ctaaatttat tatgtttgtt
    22861 gtaacttata tatttgaagt aatcctatct cgacattttc cacatttaac cttttaatta
    22921 aataaaaaat ggatatactt ctattaacaa ctaatactag ctgactaaca caagcttgta
    22981 ataacttgta gttatcatta actctacaat tctagtattt aattttatac tttagaa
//
LOCUS       DAELSY010000059        22827 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.059, whole genome shotgun sequence.
ACCESSION   DAELSY010000059 DAELSY010000000
VERSION     DAELSY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22827
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.059"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..640)
                     /locus_tag="KPG09_001936"
     CDS             complement(<1..640)
                     /locus_tag="KPG09_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBG0966546.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVSYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYKQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLHLNPEVFKNDALIYTNIDEVSIY"
     gene            complement(1034..1225)
                     /locus_tag="KPG09_001937"
     CDS             complement(1034..1225)
                     /locus_tag="KPG09_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966547.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            1445..2491
                     /gene="cobT"
                     /locus_tag="KPG09_001938"
     CDS             1445..2491
                     /gene="cobT"
                     /locus_tag="KPG09_001938"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBG0966548.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            2523..3083
                     /gene="cobU"
                     /locus_tag="KPG09_001939"
     CDS             2523..3083
                     /gene="cobU"
                     /locus_tag="KPG09_001939"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBG0966549.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            3093..3866
                     /gene="cobS"
                     /locus_tag="KPG09_001940"
     CDS             3093..3866
                     /gene="cobS"
                     /locus_tag="KPG09_001940"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBG0966550.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWIIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            3886..4500
                     /locus_tag="KPG09_001941"
     CDS             3886..4500
                     /locus_tag="KPG09_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG0966551.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      4567..4746
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4876..6375
                     /locus_tag="KPG09_001942"
     CDS             4876..6375
                     /locus_tag="KPG09_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBG0966552.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVGVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            6414..7790
                     /locus_tag="KPG09_001943"
     CDS             6414..7790
                     /locus_tag="KPG09_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBG0966553.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDASVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            7791..8762
                     /locus_tag="KPG09_001944"
     CDS             7791..8762
                     /locus_tag="KPG09_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG0966554.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYVSSFVSMLVFTLIHVLCRMILINLII"
     gene            8794..9864
                     /locus_tag="KPG09_001945"
     CDS             8794..9864
                     /locus_tag="KPG09_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0966555.1"
                     /translation="MKDLGHGANVGEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIGINPDFIIPGNGATEVIYLLMKSVKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLGKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRAYKKTTKELKKDLLKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            9866..10753
                     /locus_tag="KPG09_001946"
     CDS             9866..10753
                     /locus_tag="KPG09_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG0966556.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSEVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVIILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDTLCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            10741..11373
                     /locus_tag="KPG09_001947"
     CDS             10741..11373
                     /locus_tag="KPG09_001947"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBG0966557.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            11382..12608
                     /locus_tag="KPG09_001948"
     CDS             11382..12608
                     /locus_tag="KPG09_001948"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBG0966558.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCKERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            12605..13213
                     /locus_tag="KPG09_001949"
     CDS             12605..13213
                     /locus_tag="KPG09_001949"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBG0966559.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNVVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKIPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            13203..13778
                     /locus_tag="KPG09_001950"
     CDS             13203..13778
                     /locus_tag="KPG09_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBG0966560.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNIKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            13771..14523
                     /locus_tag="KPG09_001951"
     CDS             13771..14523
                     /locus_tag="KPG09_001951"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBG0966561.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            14507..15628
                     /gene="cbiG"
                     /locus_tag="KPG09_001952"
     CDS             14507..15628
                     /gene="cbiG"
                     /locus_tag="KPG09_001952"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBG0966562.1"
                     /translation="MNIEGNIAIICITENGKNLAVKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIIFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSIDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPIAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            15625..16350
                     /gene="cobJ"
                     /locus_tag="KPG09_001953"
     CDS             15625..16350
                     /gene="cobJ"
                     /locus_tag="KPG09_001953"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBG0966563.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            16347..17099
                     /gene="cobK"
                     /locus_tag="KPG09_001954"
     CDS             16347..17099
                     /gene="cobK"
                     /locus_tag="KPG09_001954"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBG0966564.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            17118..18596
                     /locus_tag="KPG09_001955"
     CDS             17118..18596
                     /locus_tag="KPG09_001955"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBG0966565.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEEDGFDVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            18626..19531
                     /gene="hemC"
                     /locus_tag="KPG09_001956"
     CDS             18626..19531
                     /gene="hemC"
                     /locus_tag="KPG09_001956"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBG0966566.1"
                     /translation="MNIVVGTRGSTLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            19521..21020
                     /gene="cobA"
                     /locus_tag="KPG09_001957"
     CDS             19521..21020
                     /gene="cobA"
                     /locus_tag="KPG09_001957"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBG0966567.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEDIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDNDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEDKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            21037..22002
                     /gene="hemB"
                     /locus_tag="KPG09_001958"
     CDS             21037..22002
                     /gene="hemB"
                     /locus_tag="KPG09_001958"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBG0966568.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     NSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            22155..22733
                     /locus_tag="KPG09_001959"
     CDS             22155..22733
                     /locus_tag="KPG09_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBG0966569.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCRAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT         9331 a         2218 c         4250 g         7028 t
ORIGIN      
        1 tatatataga cacttcatct atgttggtgt atatcaaagc atcattcttg aatacttctg
       61 gatttaaatg taagtttttt aattttccaa taggtattat atcatctgta aaagaaccat
      121 ttaaattgat tttttctaca tcatttttac ttttaaactc aaggtagtgt ttagaaaaat
      181 cattttgttt gtaaaaagga aggttgaatg caggatgtcc acctatagaa aagaatatat
      241 ctttagagtc agtatttttt acaatccatt ctacctcaat actattatca tttaatgtat
      301 agtttatgat tagctcaaaa gaatatggat acttctttaa agtggattca ttatctgtta
      361 atttataaga tacaaatgta tctcctttat ctacaatctc aaagtccata tctctagcaa
      421 acccatgttg agtcatgcta tataaatttt cttctattat tgtttcatta tcaaaaagtt
      481 tacctactat agggaacaat ataggtgatt gtctacccca atatttttta ttaccatccc
      541 aaagaatgtt tttgtcgtat tttttagaaa atattttagt tagttcagca ccaaaactgc
      601 ttgattcgat aattaaattt tcattttgta atatattcat ttaaaatctc ctttattata
      661 ttttatgagt atattatact ataaagtata aagattatta aattttattt gatattatat
      721 ttgaaaggta gattttagta tattttgaaa attttgtttt gaaaatttgg gattatttaa
      781 gaaattttgt attataaata gaaggaaata gataaattgt atagaataaa atatattaag
      841 ataagttaat aatgttaaat gggaagtgag tttatggaaa aaccaaatac aaaagttgtt
      901 aactttcaag agtataaaga cagaaaaaac caaagagaag taaatagaga cgaactttta
      961 aagcttataa aaaaaattgt gacttcaaaa tagggagttg ttaaactccc tattttaatg
     1021 ggtttcataa taattaagac attctgagtc catctatata atctttgaat tgtgagaaat
     1081 ctgattcact acttaattct atggtttctg aaccttccgt ataaaaattc ttaagataga
     1141 tagtataatt attatcaaca caatcatgat aaatacatct atatccatct tcatacaaac
     1201 tttttaactt agaaaaatca tccacaatat catctcctta aagttctttt aaatatattt
     1261 ttacaaaaag catctaattg tatgcaaatt gacaatgtaa aataaaggaa atgaacataa
     1321 aatgtagaat aatttttata tgtatgtaaa aataaaaaga aaaagttgaa atttttagaa
     1381 aataagagta ttttttatct aaaaagagag tttaaaaaat aaaaaaatgt tgggaggata
     1441 aaaaatgagt ttattagaaa gtatatcaaa aaacatatat tccctagata actcttctat
     1501 tgaaaaaaca aagcagagat tggataggct tatacatcca actggaagct taggaaagat
     1561 agaagatatt tgcatgcaat tggcaggaat atttggcaat gaaaattttg acacaagtaa
     1621 aaaggttata attgcttttg cgggagacca tggtgtatat gaagaaggcg tagcacctga
     1681 ccctcaaaat ataacaaaat tacagtttcc aaatttttcg aaaggtctat gtggagttgg
     1741 agtaataagt aaatttgtag gggcagatgt tgtagctgta gatgtaggaa taaactgtga
     1801 tgaaaaactg gatggggtat tagactacaa gataagaaaa gggacatcaa atatggcaaa
     1861 aggtccagct atgagtaaac aagaagccat tagatgttta gagataggta ttgaaatagc
     1921 agagcagtgt atagaaaaag attataaggt tattggtatt ggggagatgg gtatagcaaa
     1981 tactactcca agtacagcaa taatatctgt aatttcaggg tgtgacccgt tagaagttac
     2041 tggtataggt gcaggtctta agaaagaaag actaaagcat aaggctgaag taattagaaa
     2101 ggctattgaa attaataatc caaatccaac tgatggagta gatattttat ctaaagtagg
     2161 aggatttgaa ataggctcaa tggcaggtgt tatattaggt tgtagtgcta ataggattcc
     2221 agtagtaatt gatgggttta tttcttatgc agcagcatta attgcatata agataaatcc
     2281 aaaaactaga gaatatatga tagcatctca tttatcagca gaatcaggaa ctaaaagagc
     2341 attagacata ttaaaattag accctctatt aaatatggat atgagattag gtgagggaag
     2401 tggagctgct ttagcatttg atataataga agcttcaaac tatacttaca aaaatatggc
     2461 aacttttgat gaaattgaca tgggaagata aatataaaaa tttaatatgt aggagtgttt
     2521 tgatgagtaa aattattcta gtaacaggag gagctaggtc agggaagagt aactttgcag
     2581 aaaaactatg ttttgacaga aataataata cagcttatat agcaacctct attccatttg
     2641 atgatgaaat gaaagataga gtaaaaaaac atcaagagaa tagacctaga aactggaata
     2701 cttatgaaat atatgaagat atatattcca taattaagga gatatataaa gaacatgaaa
     2761 ctgttatact agattgtgta acattattag taaataatct aatgtttgaa cacaatctaa
     2821 atatagaaga gtctagtcaa gaagaaataa acaaattaga acaatacata aaagaacaag
     2881 ttgtcaaact tattgaagaa atagaaaaaa caaatctata ctttgtattt gtaacgaatg
     2941 agttaggtat gggaattgtt cctgggaata aacttagtag aatttatgct gatattgtag
     3001 gtcgtataaa tcaatatata gcttcgaaaa gtaatgaagt ttattttgta gttagtggca
     3061 tagctatgaa gataaaggag tagtatgcaa ttatgaaaag atttatttta atactacagt
     3121 ttttgacaag gatacctata aaattaaatg taggatttga tgatgaattt tataagtcaa
     3181 tagtatattt tccacttgta ggttttgtca taggtatatt atcctattta ataggatgga
     3241 tttctatgct tttatttgaa ccatttatag cttctataat aataactcta gctggtgtct
     3301 taattactgg aggacttcat atagatggat taggagatac atttgatgca atatatagtt
     3361 atagggataa agaaaaaatg cttgaaataa tgaaagattc aagattaggt acaaattcac
     3421 ttctagcaat tatgtttgta cttttattaa aggttggatt cgtttacgat ataataagta
     3481 ataattcatt gtggattata atttttatgc caatgattgc aaggttagga gtaatgttat
     3541 tgacatataa aaccgtgaca ccaagagaaa aaggaatggg aaatttattt ataggaaaat
     3601 taactacaag tatgttaata actgctataa tatacacatt attgatagtt gctcttatta
     3661 ctaaatttat atttttatta cctaatatag tgttgattaa agtattaggt tcgattatag
     3721 tggtttttgt atttataata ttattcaaaa aacatattta taaaaaaatt gacggagtaa
     3781 caggagatat attaggctgt ggaatagaac tttcagagct tgtatattta atttacatat
     3841 atttgttaat ttttatgttt ttttaaagta aaaagtggtg ataatatgat aagattaata
     3901 ttaataagac atgcactgac aaatgataat aaaaaaggaa gattatcagg ccatataaat
     3961 agttgtatta gtgaagaggg caaactacaa ataaataaaa ttaccagata tctgagtaat
     4021 gaaaacatag ataaggttta tacaacacca tctacacgta ctaaagatac agtagaaaaa
     4081 atatcaaaat tgaaattatt agaaatagaa gaaaaagaag ctttaagaga aattagtttt
     4141 ggagactttg aaggaagaac ttttgaagaa attaaaatca aatatccaaa tgagtttgaa
     4201 aaaatgataa aagaaggtaa caactataga tatccaaatg gagaaagttt gatagactct
     4261 tataaaagag tggcaaaaga aattgataat ataattttag aaaatgatag taatttagat
     4321 acaaagacaa tacttatatg ttctcatgca ggaacaataa gaaatataat aacacatttg
     4381 atttctggta gttataagta tcactggaac tttaaaattg ataatgcatc tattacagtt
     4441 ttagaaattg atggtggatt tgctgtaata gataaaatga attttacaga cttcatataa
     4501 tttaatcaaa accaaacatt acaatctgtg tttgtaatat atctgtaaaa ttagcaatga
     4561 aaatagaata ttagattaaa attaggttct taattagatg ttaaaaggga aaaaggttag
     4621 atgcctttgc agcccccgct actgtgaaac caacgaaact ataaatacca ctgtcagttt
     4681 gatgggaagg ttatagaagt aggatgaagt taagccagga tacctgccta atttaattta
     4741 aaacaagagg tcttcggggt gaaagatttt taattaatat atataaaagt cctatcttta
     4801 gaagtattag gacttttttt aatttgtttt ttagtaaatt ttaacaagga gggatggaaa
     4861 gtggggaaaa aaataatgct acaaggaaca gcttcaaatg taggtaaaag tgtcttagta
     4921 gcaggtttgt gtagaatatt taagcaagat ggttatactg ttgctccttt taagtcacaa
     4981 aatatggctt taaattcttt tataactaaa gaaggattag aaatgggaag agcacaagta
     5041 tttcaagcag aggctgcgaa aatagagcca atagctgata tgaatccagt tttattaaaa
     5101 ccttctggga ataataaaag tcaagttatt gtaagaggta aagttgtagg agaaatgcct
     5161 tcttctaagt atcatgatta taaattggag ttagtaggtg tgttgaagga aacatttaat
     5221 agcttaaatt caaggtatga tattgtagtt atggaagggg caggaagtaa tgcagagata
     5281 aacttaaaag atagagatat ctctaatatg ggtatggcag aaatagctga tgcacctgta
     5341 ataatagtcg gagatattga tagaggaggc gtatttgcct cattagcagg aacaatgctt
     5401 ttattaaatg aagatgagaa aagaagagta aaaggtgtaa taatcaataa attcagagga
     5461 aaaaaagagc ttttacaaga tggtgtaaag atgctagaag acatcataaa agtacctgtt
     5521 ataggagtag taccttatac agatataaaa atagaagatg aagacagtgt aactacgaga
     5581 tttaagaaaa aaatgaataa aagtgatata cacatagaaa taattagaac cccacatatg
     5641 tcaaatttta cagattttaa tatatttgag acacaagaag atgtaagtat tagatatgta
     5701 gactatggag aatctttagg aaatccagat atattaataa tacctggaac taaaagtact
     5761 atagacgatt taacatatat aagaaaaagt ggtcttgaat cgcaaatcaa aaatttacat
     5821 agtcaaggta aattgatatt tgggatatgt ggtggatacc aaatgttagg taaaaaatta
     5881 aaagaccctt atcatgttga aagtgaaatt gaagaagttg atggaatagg tcttttagat
     5941 acagaaacaa cgtttgagaa agaaaagact acaacacaag tagaggctac tattgttgaa
     6001 tgtatcagtg aatacatgaa taaccttaag ggaaaaaaag taaaaggtta tgaaattcat
     6061 atgggcatta caaagcgagg taatgatgta agaagtttag atttaataac taaaaaactt
     6121 gataaagaag tgaattatac tgaaggaagt gtaaataaag aggggaatgt aattgggact
     6181 tatcttcatg gaatatttga tgaaataagc tttacaagag caattttaaa caatataaga
     6241 aagaaaaaag gtttaggaga attagaaagc aaagtaaact cttttaatga atttaaggat
     6301 aaagagtatg ataagttggc tgatttttta agagaacatt tagatataga gaaaatatat
     6361 gagattatga aataatgatg acctttattt ataatttagg taggaggtgt attatgaaaa
     6421 aaattttaat tgctggtact aatagtggag taggtaaaac aacaatatca ttaggaataa
     6481 tgcaagcttt ggttaagaga aatatgaaag tacagccata taaagtaggg ccagactata
     6541 tagacccttc atatcacact tttataactg gaagacattc tagaaattta gattcttata
     6601 tgttagatga tgaaaaaata aagtatatag taaataagtc ctcaaaagat gcagatattt
     6661 cagtaataga aggtgttatg ggcttatatg atggttttgg aatagattta gataattgta
     6721 caagttcaca tacttcaaag gtattaaagt cacctgttat attagttata aatggtaaat
     6781 ctatggcagc atctagtgct gctatggtac ttggatataa ggaattagat aaagatgtaa
     6841 atataaaggg tgttatagta aataatgtaa agactaatag ccattatcaa ataattaaga
     6901 gttctataga aaaatattgt aatgttgaag tattggggta ttttcctcca aataacaaat
     6961 tctctttaga atctcgacat ctaggattag ttcctagtgt agaaatgaaa tcactaaggg
     7021 aaaaatttta taccttagca gatgaaattg aacactatat agatattgac agaataattg
     7081 aaatatcaga aagcgatgaa tttgagagtg attttgattt tcaaactttg atagaaaata
     7141 ataaaataaa agaacatgtt aataataaat caatagctat agcatatgat aaagcattca
     7201 atttttatta tagagaaaat atagagcttt tagaggaact tggtttagaa ataaactatt
     7261 ttagccctct tgaagatgca tcagtgccaa atgcagatta tatttatatt ggaggaggtt
     7321 ttccagaaat atttggaaaa gaattagaag caaatgagtc aatgagaact tccatattag
     7381 aagcacataa caataatata cctatttatg cagaatgtgg aggactgatg tacctaggtg
     7441 agaaactatt gaatcaagaa gaacaagaat ttaatatggt tggaatattt agtggaatta
     7501 gtaagatgac atcttcttta aagagatttg ggtactgtat tggtgtagcc aaggaaaata
     7561 ctcttttatc taaaaaaggg gaatctatta aagggcatga gtttcatcat tcaatatttg
     7621 aaagtgatga aaaatgtgta tattcaatga gaaaagaaag agatggaaat atagtagaag
     7681 agtgggatgg tggttatagt aaaggaaaca ctttggctac atatcttcat actcattttt
     7741 ataataactt aaattgtata gaaaattttt tgaaaaaagg aaatgaataa atgagtattt
     7801 tatctattta tataggatat gtgacagact taattgtagg agacccatat tcttttcctc
     7861 atccagtgag atttattggt aaacttataa attttactca aggtataata agaaaggtct
     7921 ctaaaaatga taggcagtta aaatttgggg gatttatatt atggtttata accgttggaa
     7981 taacatattt aacaacttat gctatagtga agctatttag ttttaatata ttattgagta
     8041 ttgtagtgaa tagttttata atttatacaa cactagccac aaaatgttta aaagacgaag
     8101 cattaaaaat ttataatgta ttaaaaaccg aagatataga aaaatctagg attcagctat
     8161 cttatatagt aggtagggat actactaatt taagtgaacc tgaaataata agagcaactg
     8221 tagaaactgt tgcagaaaat actgtagatg gtataattgc gcctatgttt tatgcattta
     8281 ttggaggagc accacttgct atggcctata aagctataaa tacattagac tcaacagttg
     8341 gatataaaaa taaaaagtat aaagatattg gttttgcatc tgctaagatt gatgatatag
     8401 ccaattatat accagctaga atatctgtta ttcttatgac aattggaagt ttctttttaa
     8461 attataacta taggaactgt tttaaaatct ctattagaga tagaaagaat cataaaagtc
     8521 caaattgtgc tttttcagaa ggcgcagtat ctggtgctct aggaatacaa ctaggaggta
     8581 ccaacgttta ttttggggaa aaagtatata agccaacaat aggggataag cttagggaga
     8641 tagataaaga agatataatt aaaactaata aaattatgta tgtatcttca tttgtatcta
     8701 tgttggtatt tacactaata catgtgttat gtaggatgat tttaattaac ttaattatat
     8761 aaattattgt ttgctaactt aagggggaca tagatgaagg atttagggca tggtgccaat
     8821 gtaggtgaaa tggcaagatt atatgggaaa aacccaaaag aaataattga ttttagttca
     8881 aacataaatc caaatgtatt accaaattta gagagataca ttttaaaagg attagaagag
     8941 tgtaggaatt atccagatat aaattataca aacttgagag aaaatatatc aaaatatata
     9001 ggtataaatc cagactttat aatacctgga aatggtgcaa cagaagtgat ttatttgctt
     9061 atgaagagtg ttaaaaaaaa attggctata ataaatccga ctttttcaga gtatagaaga
     9121 agtgctgagt taaataattt agatattata gatttagagc tagatttaga aaataacttt
     9181 aaacttaata tagatataat aaaagaaaat ataaaaagat ttgatagttt atttatatgt
     9241 aatcctaata atcctagtgg taatgtccaa gatttaaagg aacttgttca tttactaggt
     9301 aaacataata aggtactaat aattgatgaa actttcatgg aatttgttga agatgaaagt
     9361 aaatacagtt tggttaaata tatagaatca aataaaaata tatttataat taaagctgtg
     9421 actaagtttt ttggtatgcc tggtttaagg ctaggatatg gtcttacaag caacactgaa
     9481 attatgaaca aaatttatga acacaaagag ccatggacta ttaattcttt tgcagatata
     9541 ctatccaatt ttatttttga agataaagag tatattaaaa atagtaaaga atattacata
     9601 gaagaaagaa aatatatgtt acaagagttg agaaacataa gaaatataaa ggtctatgat
     9661 acagatgcta attttatttt gataagagca tataaaaaaa caactaaaga attgaaaaaa
     9721 gatttgctta agcaaggaaa catattagtt agagatgcgt ctaattttat aggattagat
     9781 gatagtttta taagagtggc tataaaatct catgaagata acaaaattct tatagaaaat
     9841 atgaaaaatt tattgggtga ttaaaatgaa atcttatgga atatgcccag catcttgtgg
     9901 agaatttgta caaggtataa ttgatgatga agaatattta tgttcatatg caatagatat
     9961 gtattctgaa gtgtatattg aagaaaaatt agtagatata aatttaggga gatataagtc
    10021 aaggttagca attgaaaagg tatttgaaaa atttaattta cctaaaaaat atactaaaaa
    10081 tatttcttta aatataaata gtaaaatacc tgttggaaaa ggaatggcaa gttcaactgc
    10141 tgatatagga gctacaataa aggcaacatt atcattgata gataaagatt taagtagtga
    10201 ggagatttct aaattagcag cagaaataga accaacagat tctatattta tagataaaaa
    10261 tagtatattt aatcctctaa atggaactgt tataaaatac ctaggaaatc tgaccaatgc
    10321 gaaagttata atacttgaac caaacaaagt actagatact atgaaaatta gattgaggca
    10381 agattataat aaattaaaag tagaaaataa agaagtaata aaaaaatcat ttgctctttt
    10441 agaagaagga ttgaaaaaaa ataatttatc tctagttgga gaggcttgta ctttgagtag
    10501 cttagcaaat gagaatatag aaaaaaaaga gtatttaaat gaaataataa aaatatcaaa
    10561 aaaatatgga gcatatggag taaatattgc tcacagtgga actgtagtag gtattttaat
    10621 tgataagtct atgaatgata aaaagatgat agatacctta tgtgaatcta acataaattc
    10681 tgtctataat aaaatttata cacaaaacat aattaatgga ggaattaagg gggaaataga
    10741 atggaataca taaagaatcc tatgaaaata gaagaaaaaa gttttgaatt gatacaagaa
    10801 ataatagacg aaattagacc agattataaa tttaagaata atattgaaga aaaaataata
    10861 aaaagagcta tacatactac tgctgatttt gattacttag atatattaaa aatatcagaa
    10921 gaagcagttg atagtataat agatgcatta aaaaataatg caagtattta tactgataca
    10981 aatatggctc ttagtggtat aaataaaaga aagttagaat cattaggatg taagtataaa
    11041 tgtttagtgg cagatgatga aactgtaaaa ttagctaaag aaaaaggaat tactcgttct
    11101 atggcagcag tagaaattgc agctaaagaa gaaggaagaa aaatattcgt tttaggtaat
    11161 gctccaacag ctttatataa agttatggag atgaaatcag aaggtagatt agatttagat
    11221 gcagttatag gagttcctgt aggttttgta ggagcacaag aatcaaaaga tgaagttgaa
    11281 aaaacagata taccatatat aatttctaaa ggtagaaaag gtggcagtaa tttagcagca
    11341 gcaatagtaa atgctatatt atatactatg taggtaccta tatggaagaa tatgtatata
    11401 tagatggaaa aaaatataga agaggatata ctacaggttc atgtgcaaca ggtgcatcta
    11461 aggctgctgt atatatgttg attacaaaaa atagaatcaa tactataaat atagatactc
    11521 ctaaggggat accattacta ctaaaagttg ataatataaa tatttctgat acatttgtgg
    11581 aatgttccat aaaaaaagat ggtggagatg atatagatgc aactcatacc atggatattt
    11641 atgcaagagc tgaaattgta gctaaaaatg ataagaacaa agggtatctt actctaaagg
    11701 atatagatag tttaagcacc aatagtgagt gtaaaagtga gctctataag tttattaggg
    11761 tttatggcgg aactggtatt ggtgtggtta cgaaaaaagg gcttagtgtg gatgttggta
    11821 aacctgctat aaatccgact ccacttaaaa tgataaacca tgaaataaga aagcttatag
    11881 gagataattt tgaatctata cttggaaatg ataaagtttt aaaaataaca atctttgctc
    11941 cacaaggaga aactgtagca aaaaaaacat ttaatccaag attaggtata gttggaggaa
    12001 tatctataat aggtacaact gggattgttg aacctatgag tgatgagggt tggaagaagt
    12061 ctctttctat agaacttcag atgaaaaaag aacaaggctt agataaaata atattggttc
    12121 caggaaatca tggagaacag tttataagag aaaaattaaa cttagatatt aagtatgtag
    12181 ttagggtaag taattttata ggctatatga ttaaagaagc tcaaagaata ggatataaaa
    12241 aaatcttaat ggcaggtcat ataggtaaat ttataaaagt atctgctggt atttttaata
    12301 ctcatagtaa ggtagcagat gctagaagtg agatattagt ggcaaactta gctttaatgg
    12361 gagctagata cgaattttta aataaaataa atcaatgcgt aactactgaa gaagctgtag
    12421 agctaataaa taatagtgaa tatagagaag tatataatat attaagtaat aaatgtaagg
    12481 agagagtaaa gcaatacttg aatgaagatt cagatgatat tgatgtggaa gtaatcatat
    12541 tttcaatgga taagtcttta ttaggtaaat cggataatac agatgattta gtggaggtct
    12601 ttatatgata aacataatag gtctaggacc tggtaactta gactatataa caaaaaaagg
    12661 tgaaaattta atttccactt ctgatgtatt aattggtggt aaaagaaatc tagagtctat
    12721 aaaaaatttt gaaggagaaa aaatagttct ggattctaat ttaagagaaa ttatagagta
    12781 cataaataac aataaagaaa agcaaatatc aataatagca tctggggacc cattaatata
    12841 tgggataggt agatatctat ctaaaaatat tgataataag atgttaaacg tggtttctgg
    12901 gattagttca ttacagtaca tattttcaaa aatatatgta gatatgaatg atgtatatat
    12961 tacaagtagt catggcaaaa taccagattt tgattacata ttatcacata aaaaagtatg
    13021 tatggttaca gattcaaaga taggaccaaa acaaataagt agagaaataa tagatagaaa
    13081 tctaaataaa attattgttg taggagaaaa tctgtcatat gacaatgaaa aaataacaat
    13141 agcgaaacca gaagagataa taagaatgga caattttgat atgaatgttg tggtgatttt
    13201 agatgaggaa tagtgaattt ataacaggta aagttccaat tactaaggaa gaggttagag
    13261 caatatcaat aagtaagtta aatttagtaa atgctaaaaa cctcatagac ataggggctg
    13321 gtacaggaag tgtaagtgtt gaagctgcat ataattatcc taatttaaag gttatatcta
    13381 tagagaaaaa tgatgacgct atagagctta tagagaaaaa tataaaaaaa ttcaacttaa
    13441 aaaatataga agtgataaaa ggatatgctc caattaatct agggttaaat gttaaagttg
    13501 agtctatttt tcttggggga acaggaaata atctggaaga aataatagaa tggtctaaaa
    13561 aaaatctcgt tacaggagga agacttgtag ctaattttat aataatagat acatttaatc
    13621 aaactttaaa actgcttaaa aagcatggat ttaaagaaat agatgtatgt gttttgaatg
    13681 tttctaaact agagaaacta ggaaaaggtg aatattttaa accactaaat cctatatata
    13741 taatatcttg tgaaaaaggg gaaaatgatg atgaataaag tacattttgt aggagcagga
    13801 cctggagata aagagctgat aacattaaaa ggatacaaat tattaagtaa tgcagatgta
    13861 gtgatatatg caggttcact tgttaatcct gaacttttgg aatactgtaa agaagattgc
    13921 caaatacata atagtgcaca tatggattta caagagataa tagatgttat gagagaaggt
    13981 atagaaaata ataaatctgt tgtaagactg caaacaggtg atttttctat atatggttca
    14041 attagagaac aagtggaaga tttaaataaa ttgaatatag attatgactg tactcctgga
    14101 gtaagttctt ttttaggggc agcatcatct ttaggggttg aatatacagt tccagaaatt
    14161 tctcaaagtg taattataac aagaatggaa ggcagaactc cggttccaga gaaagaatca
    14221 atacaatcat atgctaagca tcagacatct atggtaatat ttttatctgt tcaagaaatt
    14281 gagaaagttg tgtctaaatt actggaaggt ggatatccaa aagatactcc tatagcagtt
    14341 atatacaaag caacttgggc agatgaaaaa atagttaaag gaactttaag tgatatagct
    14401 gtaaaagtta aagaaaacaa cataaataag actgctttaa taatggttgg gagattttta
    14461 ggagaagagt ataataattc aaagttatat gataaggatt tcaaacatga atatagaggg
    14521 taatattgct ataatctgta ttacagaaaa tggaaaaaat ttagctgtaa agataaattc
    14581 tctagtaaat aactgtgtta tatatcaagt aaaaaataaa aaaagtaatt tagttgttga
    14641 agaaacatgt gtacatacag ttcaaaagaa gttaagtgat tttgtaggag aaatatttag
    14701 caaatttgat tacataatat ttataatggc tacaggtata gttgtacgaa gtatagcacc
    14761 atacataata agtaagttta gtgaccctgc aattttggta atggatgaaa aaggcaaaaa
    14821 cgtaataagt ttattgagtg gtcatattgg aggggcaaac agcttgacac agtacataag
    14881 tagtcttatt gatgctaatc cagtaataac aacagctact gatataaatc aaaaagcttc
    14941 cttagatatg atagcaaaaa aactaaatgc acatattgat aattttagag aaagtgtaaa
    15001 agaagtaaat tctatgctag tgaataataa atgtgttgga ttatatgttg atgaaggata
    15061 tcaagttgat actaggggat ttaaacttat agaaagtaag gatgagttaa gagatagctt
    15121 ttcaaacaca tatatagaaa aattagttgt agtaagcaat aaaataaatt gtattgaaga
    15181 tattgagaat ttaaacttag ataataaatt tgaacattgc tatagtacta aaaaaataat
    15241 aaaggtaatt ccaagagata tagtgatagg aattggctgt agaagaaaca cagatagtga
    15301 gctgatgtat aattcacttg aagagtttct taataaacaa aatatagata ttagagcaat
    15361 aaacacagtt ggaagtatag aactgaaaaa agacgaaaaa gcaataattg aattatcaaa
    15421 aatactaaaa gtgccattta aaacattttc aatagatgaa atatctaagg ttgataatct
    15481 ttttgataaa tctgattttg ttaaaaaaaa tgtgggcgtg tattcagtag cagaacctat
    15541 agcatatctt ttgagcaaag gtaatttgtt agttgaaaaa cataaatata aaggaataac
    15601 tttttcagtg gggagaataa aaatatgata tacgttgtag gaattggacc aggaagtaaa
    15661 gatacaatga ctttagaagc aataaaagca atagaagatt ctgaggctat tgttggatat
    15721 aagacttata taaaactaat agaagaattt atacaaggta aagaaataat ccaaaatggt
    15781 atgaggcaag aagttgatag atgtaaacaa gcagtagagg aagcgaaaaa aggaaaaaaa
    15841 gtagcagttg taagcagtgg tgatgctgga atatatggga tggcaggtct cattttagaa
    15901 ttaatatcta aagaagatga agatataaaa gtaaaggtaa ttccaggtgt tactgcaagt
    15961 attggtgcag cagctatact tggagctcct attatgcatg atttctgtca tataagtttg
    16021 agtgatttaa tgactccgtg ggaagtaatt gaaaaaagat tgagattggc tgctgaagcc
    16081 gattttgtaa tttgtttgta taatccaaga agtaagggaa gaagtgaaca tctaagcaaa
    16141 gcatttaaaa taatgggtga gtttaaaggt ggagatacac ctgttggagt ggtaaaagat
    16201 gtaggtagag aaaaagaaga aaagtttgta tgtacttttg aaaatatgga ctttgaaaaa
    16261 gtcgatatga ctacaatggt tataatagga aataagtcta cctatattaa tggtgaattg
    16321 atgataactc caagaggtta tacagtatga ttttaatttt aggaggaact tcagatagtt
    16381 tatctatatg tgataagata aatgaactga aaaatcaacc atatatcctt tcagttacta
    16441 cagattatgg aagggaactt gcacaacaac atgcagagaa tgtaatttta ggtaagcttg
    16501 gaaaagaaga tatgctaaaa tttataaaag ataataatat agtaaaaata atagatgcca
    16561 ctcatcctta tgcagttgaa gtatccaaaa ctgctattac ttgtgcaaaa ttattgaata
    16621 tagattatat aaggtttgag agaaaatctt taattgaaga aatagattat gataataaat
    16681 ttatagtaga tacaattgaa gaagcctgtg aaatagcaaa taaaataggg acaaatatat
    16741 tcataggaac aggaagtaaa aatttgaaca gatttataga ggaaataagt gataaaaatt
    16801 tagttgttag agttcttcca acgagtgaag tcatattaag ttgtgaaaaa ttaggtttaa
    16861 gtgcagataa tatagtagct atgaaaggtc catttagcca gtatataaat gaagaaacat
    16921 ataaacatta caatatagat ttagtcataa caaaagaaag tggaatagct ggtgggttct
    16981 tagagaaggt aaatgcatgt aaaactttca atataccagt aataattata aaaagagaat
    17041 cgattaacta cgaaaatgta tttaatgaca taaataaaat taaagacata cttgtgtaat
    17101 ttttaggagg agaatatatg aagaaagcct tattagtagt gagttttggt acaagctatc
    17161 atgaaacgag agaaaagaca atagatatat gtgaagataa aataaaaaat tcgttaaaaa
    17221 gccatgattt ttttagagct tatacatcaa atatgataat aaataaaata aaaaaaaggg
    17281 atggaataga aatagataat ccaatacagg ctttagataa aatatatgaa caaggatatg
    17341 atgaagtaat aatccaaaca cttcatatta tatgtggcga agaatttaac aaattaaaag
    17401 aacaagtaga tggatattct tctaaattca aaaagattgt attaggaaga cctttgctta
    17461 cacatattga agattatcag gaagctgtag aggcaattaa gcatcaaatt cctcatatgg
    17521 aaagtaatga agcagtagta tttatgggac atggaacact tcatgaatca cattcagcgt
    17581 atccagcatt ggaatacatg cttagggata atggaataaa tgcttatgtt ggtacagttg
    17641 aagggtatcc agaaatagaa catgtaataa gaagattaaa agaaggaaat ataaaaactg
    17701 taaatttgat gccatttatg ttagttgcag gagaccatgc tataaatgat atggcaggag
    17761 atgaagaaga ttcttggaaa acaatattag aagaagatgg atttgacgta agagttcatt
    17821 taaaaggact aggagaaaat tcttatatac aagataaatt tgttcgtcat gctgtaaaat
    17881 gtgcagagaa tgctaagttc tatggtatag gagcagggcc aggagatggt tctttactta
    17941 cgataaaagc ggttaatact ttaaagaaac tggatattct atacactcca gaatcaaaaa
    18001 agggtggaga tagtctagct ttatcaatag ttagtgaata tttacctgaa tcagtggaaa
    18061 taaagtctag gcatttccca atgagttttg atgggaatga aaaaagttta gcttgggaca
    18121 atgtagcaca agaaattata tcagatgtta atgatggtaa aaatgtagga tttgttacat
    18181 taggagaccc tatgatttat agtacatatg tatatataat gaaaagaata ttaggagagg
    18241 tagaagtcga aactatacca ggaatttcat cattttccaa tatagcatct aatcagaatt
    18301 ttccattagt catggataaa gaagctttag tggtaattcc ttgtactatg gaagatgata
    18361 agatagagta tgctttgcaa aattataatt caatagtatt gatgaaggta tataaaaatt
    18421 ttaaagaaat aataaaaaaa ttagaaaagt atgatttaat agaacatgct attttagtaa
    18481 gtaattcttc ccaagaaagc gaaaatgtgt ttacagattt aaaagaagcg caccttgaag
    18541 ataagatatc ttatttttct actatactag taaataaaga taataaaata aaataggtga
    18601 aagcaatatt taaaggagac ttaatatgaa tatagtggtt ggaactaggg gtagtactct
    18661 agcgcttata caaacagagt gggtaataaa tgaattaaaa aagaagtatc cagaaattag
    18721 ttttgagatt aaaatcataa agacaaaagg agatttaatt caaaatgttt cactggataa
    18781 aataggtgat aaaggattgt ttgtcaaaga aatagagcaa caattattag atggaaaaat
    18841 agacatagca gtacatagta tgaaagatat gccttcttat ttagcagatg gactaaaatt
    18901 tgctcatacg cctaagagag aagacccaag agatgttcta atacttagag aaggatataa
    18961 aaatttagat gatttgcctc atggagcagt aataggaaca ggaagtaaaa gaaggaagtt
    19021 tcaattattg aaacaaagac ctgatttaaa tatagtacaa gttagaggta atgtggaaac
    19081 taggatacga aaaataaaag atgaaaacat gcatggtatt gtgttggcag catcaggaat
    19141 aattagagct aatttgcaag ataaaataag ttcttattta cctgttgatg ttgttattcc
    19201 agcacctgcc caaggagctt tagctataga aattagaagc aatgatagta cgatagaggg
    19261 aatagtaaat tcactgaagg atgagaatac agaaatccaa atattggctg aaagaggttt
    19321 tttagatggt gtaaatggaa gttgtcatat tccaatggca gcttattgtg aaataatcca
    19381 agataaaata catcttactg gcttatatgg agattcagaa ggaaaaaaag tagtaatcaa
    19441 gtctatagat ggagatattt cttctcctag agagttaggt ttaaagttag ctaaacttgt
    19501 attgaaggag tatgagaatt atgaagggta aggtttactt agtaggggca ggaccaggtg
    19561 attataaact catgacttta aaaggtttag agtgtataag aaaatcggat gtaatagtat
    19621 atgatagatt agcaaatagt agttatttaa gagaagctaa gcctgattgt gaacttatat
    19681 atgtaggcaa ggcatcaagt aatcatattt tgccacaaga agatataaat agaattatag
    19741 cagaaaaagc taaagaagga aaaattgtaa ctcgtcttaa aggaggagac ccttatgtat
    19801 ttggcagagg gggagaagaa gcagagactt tagtagatga agatatagaa tttgaagtag
    19861 ttccaggtat aacttctgct ataggaggtt tatgttatgc gggtattcct ataacacata
    19921 gagaccatgc ttcgtcattt catgtaataa caggtcattt gaagggtgat gatagtggag
    19981 aattaaactg gaatgctctt gcaaataaca aaggtactct tgtgtttttg atgggaattt
    20041 caaacctaga gaaaataagt gaaaacttaa tgaaagaagg aaaagataag gatacacctg
    20101 ttgctcttat aagttgggca actagatata atcaaagagt tgttacatct acacttgaga
    20161 atgtttatga aactgctatt aaagaagaag taaaaccgcc aactcttata gtagttggga
    20221 gtgttgtagg acttagagaa aagttgaact tctttgaaag taagccttta tttggaaaaa
    20281 gtattgctgt aactaggtct agaaatcaaa atagtgtatt ggtagaaaaa ataatggatt
    20341 taggtggaaa tcctatagaa atacctacta ttaaggtaga aaaaatacaa aataatatta
    20401 acttagaaaa tgaaataaaa aatataaata agtataatta tttaatacta actagtaaaa
    20461 atgccgtaga aatcttcttt gaaaagatat atgaaatgaa ttttgattta aggatattat
    20521 ctaatttaaa ggtatgtgct ataggttctg ctacatcaaa tgagttgaaa aaaagaggaa
    20581 ttatagcgga tatagtacca aaaaaatttg tagcagagtc actgtatgaa gagttagctc
    20641 ctattttaaa tgataacgat aatatactta tacctagagc agaaaatgct agagattatc
    20701 tggttgacaa acttaaggct atttgtgaag taacagaagt acatatatat agaacagtaa
    20761 tagatgagga taaaaaaggt gagatattag atattttaaa ttctaatgat atagattata
    20821 taacatttac tagttcctca acagtaaata atcttgttga aattatagga aaagaaaata
    20881 ttaaaatatt agaaaaaata aaaacactat ctataggtcc tataactaca gagacaataa
    20941 atagtttggg aataaaattg tacaaagaat ctgctacatc tactatagat tcgttagtag
    21001 atttaattat aaaaaattaa ttttggaggc ttgaatatgt taagaagacc aagaagacta
    21061 agagcaagta aagcaatcag aaatttggta agagaaacta aggtgaatgt agaagattta
    21121 atatatccac tgtttgtagt agagggggaa aatataaaag aagaaatcaa ttctttacca
    21181 gatgtatatc atttatcaat agacatgcta gaagacgaaa taaaagaaat aagtgagttg
    21241 ggaattgaac atataatgtt atttggtgtt ccagatgaaa aagatgagtg tgcttcacaa
    21301 gcttttaatg ataatggaat agttcaaaag gcaattagaa aggtaaaaga aataaattca
    21361 gatataaatg taataacaga tgtctgtatg tgccaatata caagtcatgg acattgtggg
    21421 attttaactg atactggtta tgttgataat gataaatcat tggagtattt ggctaaaata
    21481 tctgtaagcc atgctaaagc aggagctgat atggttgctc catctgatat gatggatgga
    21541 agaatacaag caataagaga agctttagat gaaaatggat tagaaaatgt tggaatcatg
    21601 gcttatagtg ttaagtatgc ttcaaattat tatggacctt ttagagaggc agctcattca
    21661 gcaccatctt ttggagatag aaaaacatat caaatggacc cagcaaatag caatgaagct
    21721 ataatagaga ctgaacttga tatattggaa ggtgcagata tacttatggt taaacctgca
    21781 ttatcatatt tagatattat aagaagagtt aaagataatt ttgacttacc tctagcagct
    21841 tataatgtaa gtggagaata ctctatgctt aagtttgctg tgaaggaagg tttattaaat
    21901 gaagatgtaa ttcttgaatc aattacatct ataaaaagag caggagcaga tataataata
    21961 acttattttg ctaaagacat tgctaaactg ttgaaaaaat aaattctagt agtataaaga
    22021 atatcaaaaa gtataggaat tgtaattaac tattatacag tagttttgtt tagacattta
    22081 ttgaagaata atagaaacta atttacaatt tctatacttt atttatgtaa ctaaataaaa
    22141 tttggaggta gaagatgcta tatcctatta atttaaaatt agatgagtta gatgtagtta
    22201 taattggtgg tggagaagtt gcatatagga aatgtaaaaa ttttttagag tttaacaaaa
    22261 atgttactat agtttctaaa caaattttaa ataaatttta tgatttaaaa ggaaatataa
    22321 aaataataaa agatgattat aaagaaatat atataaaaga tgcttctgta ataattgctg
    22381 ccacaaataa tagagagtta aatatggaaa taggattata ttgtagagca aaaaataagc
    22441 tagtgaatgt agttgataat atagaaatat cgaactttac tgttccatca tatataaaaa
    22501 gaggagattt acttataagt gtgtctactg ggggcaagag tccttcatta tcatcaaaga
    22561 taaaaaaaga aattgaagag agatatactg aagattatga ggagtattta aatgtattag
    22621 gagatataag aaaaaaggtt ataaaaaaat acgaagataa aagtaaaaga aaagatgtgc
    22681 ttaatatgtt ggtaacactt gacctagaag aacttaagaa gtttgatata taaaaaataa
    22741 aataaaaaat gttaaaaagg tgttgacgga ttatttttaa gatgatatag tgttacttgt
    22801 ccttaagaaa agggcaaaca taaaaaa
//
LOCUS       DAELSY010000060        22563 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.060, whole genome shotgun sequence.
ACCESSION   DAELSY010000060 DAELSY010000000
VERSION     DAELSY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22563
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.060"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            151..1431
                     /locus_tag="KPG09_001960"
     CDS             151..1431
                     /locus_tag="KPG09_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBG0966570.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKVDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
     gene            complement(1694..3085)
                     /locus_tag="KPG09_001961"
     CDS             complement(1694..3085)
                     /locus_tag="KPG09_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG0966571.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(3096..4316)
                     /locus_tag="KPG09_001962"
     CDS             complement(3096..4316)
                     /locus_tag="KPG09_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBG0966572.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(4330..5118)
                     /locus_tag="KPG09_001963"
     CDS             complement(4330..5118)
                     /locus_tag="KPG09_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG0966573.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(5135..6409)
                     /gene="larA"
                     /locus_tag="KPG09_001964"
     CDS             complement(5135..6409)
                     /gene="larA"
                     /locus_tag="KPG09_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBG0966574.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(6527..7240)
                     /locus_tag="KPG09_001965"
     CDS             complement(6527..7240)
                     /locus_tag="KPG09_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG0966575.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            7365..8624
                     /locus_tag="KPG09_001966"
     CDS             7365..8624
                     /locus_tag="KPG09_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBG0966576.1"
                     /translation="MKKNKIIAFFAPAIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKHEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFIKKFSRKK"
     gene            8892..9458
                     /locus_tag="KPG09_001967"
     CDS             8892..9458
                     /locus_tag="KPG09_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0966577.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(9659..10864)
                     /locus_tag="KPG09_001968"
     CDS             complement(9659..10864)
                     /locus_tag="KPG09_001968"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG0966578.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(10967..11073)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11165..11533)
                     /locus_tag="KPG09_001969"
     CDS             complement(11165..11533)
                     /locus_tag="KPG09_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBG0966579.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(11585..12862)
                     /gene="obgE"
                     /locus_tag="KPG09_001970"
     CDS             complement(11585..12862)
                     /gene="obgE"
                     /locus_tag="KPG09_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBG0966580.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(12973..13263)
                     /gene="rpmA"
                     /locus_tag="KPG09_001971"
     CDS             complement(12973..13263)
                     /gene="rpmA"
                     /locus_tag="KPG09_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBG0966581.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(13277..13594)
                     /locus_tag="KPG09_001972"
     CDS             complement(13277..13594)
                     /locus_tag="KPG09_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBG0966582.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(13609..13920)
                     /gene="rplU"
                     /locus_tag="KPG09_001973"
     CDS             complement(13609..13920)
                     /gene="rplU"
                     /locus_tag="KPG09_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBG0966583.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(14021..15442)
                     /locus_tag="KPG09_001974"
     CDS             complement(14021..15442)
                     /locus_tag="KPG09_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBG0966584.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLIIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(15460..16161)
                     /locus_tag="KPG09_001975"
     CDS             complement(15460..16161)
                     /locus_tag="KPG09_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBG0966585.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(16163..18007)
                     /locus_tag="KPG09_001976"
     CDS             complement(16163..18007)
                     /locus_tag="KPG09_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBG0966586.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFI
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            18326..19519
                     /locus_tag="KPG09_001977"
     CDS             18326..19519
                     /locus_tag="KPG09_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBG0966587.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(19731..20813)
                     /locus_tag="KPG09_001978"
     CDS             complement(19731..20813)
                     /locus_tag="KPG09_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG0966588.1"
                     /translation="MKKIRKLATKLLIATMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGESVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQEEAILDEVYGNNLRPKIERYEGVPCSLINSSINTIKFTSKNSNLDPS
                     RHTVSVIGFMNTPYGMVALAPDGVPVEYPFEYDKPFSLDFTGKNLSAAELSKKCKNNN
                     VIIKIEIGEIEPNSGRGQVRYARYVN"
     gene            complement(21377..22168)
                     /locus_tag="KPG09_001979"
     CDS             complement(21377..22168)
                     /locus_tag="KPG09_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966589.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTNIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(22172..22501)
                     /locus_tag="KPG09_001980"
     CDS             complement(22172..22501)
                     /locus_tag="KPG09_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0966590.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
BASE COUNT         7261 a         3988 c         2514 g         8800 t
ORIGIN      
        1 acatttaacc agttttttat gtaggtaaca tttctacatt tttataaatt tttttacgtt
       61 ttttaatttt ataattaaaa atttaaattt tatacatatt ataataaatt gtgatatata
      121 taaaataatt aaagataaaa ggagtatttt atgaacatac ttggattaat agttgaatat
      181 aacccatttc ataatggaca catatatcat ttatctaaat ctatagaaaa aacaaatgca
      241 acacatactg tagcaataat gagtggaaat tttctacaaa gaggcgaacc agctttattt
      301 gataaatata ctagagcaaa aattgctgtc gaaaatggtg tagacttagt tatagaatta
      361 ccaactttat ttgcttgtca aagtgctgaa attttttctc atggtgctat aacaatttta
      421 aattcactaa actgcataaa ttctatatgt tttggtagtg aagaaggaaa tacagaaatt
      481 ctttataata tagctgaaat tttagcaaat gaaccaaatg aatttaagtt acttttaaaa
      541 aaatatttag atgatggcct gttatttcca acagctagag ctaatgcact ttttgattat
      601 atacatcaag acaaaaaaaa taattttata caaaattatt caaaggaaca attatcaaaa
      661 acattaaatt catcaaacaa catactgggt atagaataca taaaaaacct catccattta
      721 aacagtagta taaaaccatt tactatctcc agggtccaat cagaatataa ttccgaagaa
      781 attgagagtg atatttgttc tgctactgct ataaggactt cacttaaaga aaatacagac
      841 ctttttcatt taaaaaatgt tattccagaa aaaacttata acatcctaaa tgaaaaagta
      901 gataatggtt ttttacctat gtttgatgac ttcttctttg atattttaaa aacaattata
      961 cttagagata tggacacttt aaaagagtat tttgaagtaa atgaaggcat agaaaacaga
     1021 atctataaag atatctttac atctgaattt ctacatgagc tacaattagc aattaaatcc
     1081 aagcgataca ctttaaccaa aatcaaacga actctaaaca atatattgtt aggtattaaa
     1141 aaaagcgaca tgcaacttat aaaaaacata gatactatac cttatataag aatacttgct
     1201 tttaatgata aaggtagaga aattctaaaa gagataaaaa ataaatctga aataaaaata
     1261 ataaacaagt tttcaaatat atcattttca ttagatgata cgatttttaa aacactcata
     1321 agttatgata taaaagctac aaatatatat aattcaatat attataaaaa caataaagat
     1381 ttagtaaaag gccctatgga tttttatact catccaatat atgtaaagta gttttttaaa
     1441 ataactatat ttataaaaat cattttatta actctttatt atataagata caatagtctt
     1501 actttattta tgtacacatg cttttaggat gacaacatca acttaaatat tatgttactt
     1561 tctaaattta agtaaatatt gccatcctaa aaattttaca atttagcttc agatttaaaa
     1621 gtaaagttta aaatattata tttaaatcta atttaatatt ttttgttttg taaatattat
     1681 taaattaata tttctaatat attttccctg gatttaaaat atttttaggg tcaaaagcta
     1741 actttatatt tttaattaac atcatataag catctgaatt tgattcatgt aagtactctt
     1801 ttttagcata tcctatgcca tgttcaccag aaacttgacc actaagttct ctagattttt
     1861 tatacatata gtcaaaagtt tcttttaatc ttattttcca tgtattatca tccataccat
     1921 cttttaaaat atatatgtgt agatttccat ctcctgcatg accaaaactt tttattctta
     1981 tttttaattt gtcttgaagc tcgtgagtgt atcttataaa ctctgctatc ttatctcttg
     2041 gaacaacgac atcacattca tccatttgtg tcgtagatgc ctttatagct tctaaaaatg
     2101 cacctctggc tgaccatata gaatcgtttc tttcttgtgt atctgatata aatacatcta
     2161 atgcaccatt ttctaaacat aaattagcta ctttttcata ttccttttct atatcttcag
     2221 tgctatttcc atcaaatgta agtaaaagat aagcatctga tgtattatct ggaaactttt
     2281 tacctaaaaa ttcttctgct gccaatatta catctctttc cataaattct atagccgttg
     2341 gtattgattt tgattttatt attttaggaa cagtttctat tgccattgat aaatctggaa
     2401 atggtataag caaacttata cttttctttg gtaaaggaag caattttaat atagctttag
     2461 taactattcc taaagtacct tctgaaccta ctaataaatc ctttatacta tatccagaac
     2521 tatttttaac cacctttcca cctacattta taatttctcc atttggtagt acaacttcaa
     2581 gtcctcttac ataatccctt gtaacgccat attttactgc tctcatacca cctgcattag
     2641 tgtttatatt acctgcaatt gtagcacttt tttcacctgg gtcaggtggg taaaataaat
     2701 ctctatcttg aacatattga cctattgtca tcaataaaac acctggttct actgtcaatg
     2761 ttaaattttc ataatcaact tcaagtattt tattcatttt acatagatta atcattattc
     2821 caccatttat ggcaacagca gcacctacaa gaccagtacc ttgacctctt ggtgtgactg
     2881 gtatattatt tttatatgcg tatttcatta ttttagatac ttgttcagtt tcaagtacat
     2941 ttactaaaac ttcagggtac ttctcaatcc ctccaagttc atcatgtgaa aaatcttcat
     3001 ttatatcact tccaactaat acattttctt ctccacaagt atctattaaa aaatctatat
     3061 cttttttatc gattaattta tacatttcta tccccctatt tagctgctga tgccatatat
     3121 ttttgattat tatctttgtt attttctatt ttttcaatca atttaggaat aatatcataa
     3181 atatcaccta ttaaagctaa atgtgctaca tcaagtatag gcgctttatc atctttattt
     3241 attgctacta tatagtcagc accctgcatc cctgcaacaa attgaactgc tccagataca
     3301 ccacaagtta ttatcaattt aggttttaca gttctaccac taagacctat ttgtttttta
     3361 gcatctatcc aacctgcttc tattacaggt cttgttcctg ccacttgagc gtttagctta
     3421 tcagctaatt tatatatcat ctccatatct tcttgttttt ttattgctct gcttgccaca
     3481 actatcactt cagcttcttc cagaccaact tctttatttt tttcttttac actcagtacc
     3541 ttaattttag attttagttc ttttttttct actttacata aagttatctt tcctgtagta
     3601 ttctctattt tttctggtgc tgaaaatatt ttatatctta cagtggcaaa ttgtggtcta
     3661 ttattaggtg tgtttatatg agccattata tttccaccaa atgctggtct tatttggtct
     3721 aaatcagtat ttgattgaat atcaagtatt gtacaatctg ctgtaagacc tgttctaaat
     3781 ctagcggcaa gtcttggagc taatgaccta cctaatgtag ttccaccaac taatactatc
     3841 gttggtttta ttttatctat aaagttttct attgcttttg aatatggttc tatcctaaaa
     3901 tctttaaatt catcttcatc atatacaaat acttcatcca ccccataaga aatcaaatta
     3961 ctgcacttat cttttatgtc ttttcctaca aatacacaat acacaggttg ttttattttt
     4021 gatgccatct cacgagcttt acctataagc tcgtacgtta caggatgtat atttccatta
     4081 tgatgttcaa catatactgc aattcccctc cattcatctt tattaatttg tactttacta
     4141 ctctctataa attcaaatgc gccttttggt ccacttttga cacacaactt gcacatctta
     4201 cagccagcac ttatttctac tatacctgac ttttcttcta tagccccaaa aggacatatt
     4261 tctaaaattc tctttaagtc agttatttta tcttgattta caactatctt tgacatttat
     4321 tactccccct tacaccaact ttaattcctc taatttattt acaagtgcaa aacttagttc
     4381 atctgcattt cctcttacaa tatttttatc tgtatttggc tttggaggaa atattcttac
     4441 aacttgagta ggagaaccgt taagtccata gttattttca tctttatctt caaagtcatc
     4501 taagctatat acaggtattt ctctatcttt agtattaagt tttaatttaa atgaaggtag
     4561 tcttggttga aaaatgtctt tttctacagt tattaaacat ggatattcaa tctcacatac
     4621 ttgtaaatca tttggcatat ctatttcaac aactatactt tcatctttaa cttcaattaa
     4681 ttttgtaacg ttagtaacat gaggtatatc taaaaattca gctacttcag gtcccacctg
     4741 tgctgtatca ccatcagtag tttgttttcc acaaattatt aggtctacct ttcccatctt
     4801 cttaatacct tgtgatatgg tataggaagt agccaataca tctgctcctc caaatctcct
     4861 atcacttatt aatgctcctt catcagctcc catcataaaa ctttcttcaa ttaccttctt
     4921 tgcctgagga ggacccatgc taagtacttt tagattcgct ttcttatctt cctttattct
     4981 aagagctgtt tctaaagcat ataggtcata gggattcatt tttgaatcta ctccatctct
     5041 ttttagtact cctgtagttt catcaacttc tactgaagtc gttccaggaa cttgtttaat
     5101 acatgtcaaa atttccataa agtacactcc ttttttattt aactataact gatactccat
     5161 caggtataac tactactttg ctctccttac caactatact attcgccatt tctaaagctt
     5221 ctttaaagtt aaatgcatgt ttcatatgca ttttttttat catttcagga ttacacttat
     5281 ctgttaccat tatcacagta tatttagata atattctcgc tagtatctga aattcccatt
     5341 ggtctggaat cgtatctact ctatcgacac ttctaatttt atctaaaagt tgttttggag
     5401 aatctgcatt tgctacattt tcataaaaag attgacctcc atgaccatca ttacatgctg
     5461 aaatcattat tattactcca ccgtctttac aagtagcttc agctgctgtc attcctttaa
     5521 cagattgata aatattttgg tctagtggat atccaccatt tgtagttaca acaatatcag
     5581 attttataga atttactttt gataactctg ttacaaatct acatccttta gtatgtgctt
     5641 tttcgacatc accagcaaag gaagcaatta tttttttatc tttatctatt acaacattca
     5701 atatgaatgc taactttgca actttagctg catataccat atcctcatga ataggattat
     5761 tattcaattt cccagttctt gaattatcac tatctataaa gtcactacag tgattgtaca
     5821 ttatagtctt tgcagatgcg attcctggaa gtacgctttt tctgccacct gaaaaacctg
     5881 caaagaaatg aggttctata aaaccttctg ctatcaaaag ttctgcctca tatgctagtt
     5941 tgtttatata taaatctcca cctgaaggca atatccctac tttacacata gatttatcat
     6001 cagtagatat atgcataact atatcttcat tatctactat ttcttctccc attttataaa
     6061 ttagctcttc ccttgtactt ggcctatgaa atccagttgc cactataatc ttcacatcaa
     6121 tttctggatt aacattccta atccttctta atataattgg catagttatt ctacttggta
     6181 caggtcttgt atggtcactt gtaattatga ctacattctt tttcccctta accagttctt
     6241 ccagagaatt agagcctata ggattattca tagattcttc aactatctct tcttgagtct
     6301 tatgttctat ataattatcc gattttgact ccaaaacacc gagaaaacgt tcatcaggta
     6361 tttctaaaat cattccttgt tttgaatacg gtacttttaa ctttaccatt tttaatctcc
     6421 cattataata aactataaaa ttggtatgtg gtcataccag tttcacattt atatgatagt
     6481 accattatct accatttgca agaataattt attttataac aaacttttaa ttttcatttt
     6541 ttctataatt tttacttata aaatccatat gttcctttgc tttctcacat gcaagttcta
     6601 catcattttc tacaactgca ttgtatatag cttcatgctg tttaagcaat atattttcat
     6661 taaaaggttc tgcgattatt ttttctcttg catcttctat aaatccatca aataattttg
     6721 atgctgtcat aaaaagactt tgaattaaat aatttttact taatgttgct agtttactgt
     6781 gaaatttttg gtctaataac accagtactc gattattatc ttttttatta gtttctactc
     6841 tcatttcatc tattatatgt ttcaattcta taacttcaga agaagttatt ctctttgaag
     6901 caagttttgc aatctcaatt tctaggatta ttctaagctc taatatatct tcaagtgtgc
     6961 cattattaag tttaaaaatc atagataaag gttctattaa agttttttct atatttgtac
     7021 atataaaatt tccttcaccc tgtctgcttt ctatcactcc catagtctct aataccctaa
     7081 tagcttccct tatggaagtt ctactcacat tcatttgttc agacaattct ctttctgata
     7141 gtaatttatc cccctttttt aactctccat ttagtatttt atattgtatt tgctcaatta
     7201 cttgttcata aactttttta ctagctatac tattaaacat tttttcctcc aatatgataa
     7261 tcgttgttgt aatatatatc tattatatat cttaaaccat tacattcata tatttttatt
     7321 acttttaata taatatctta taactcaaat ttacaggtga tattatgaaa aaaaataaaa
     7381 ttattgcttt ttttgctcca gctattatag ttggagttct agttttaggg attatcctat
     7441 acccaaatga agctataaaa tctgcaagag aaggcttctc tatatggtct aatgttttag
     7501 ttccatcact actaccattt ataattggag caaacttaat tgttgattta aaaatagtgg
     7561 atattatagg gtttatcatc aatcccataa ctagatttgt atttaatgtt tcaggaaaga
     7621 gcgctttagc ttttgctata tctacagtat ctggttatcc agtaggagta agtttagctt
     7681 ctgaatttag gagtaatggg caaatttcta agcatgaagc acaaagactt gtgtcttttt
     7741 gttctacatc aggaccttta ttcataatag gttcggttgg tacaggtatg ttccaaaatt
     7801 caaacttagg ttatttaatg attctctgcc attatctagg aactatattg gttggtcttt
     7861 tctttagaaa ttatggtaat gaaaatcttc caaagacaaa attggattta aaaacaaata
     7921 taaaaaatgt aatcaacatt agaaattcaa gtgggaatgg tttttttgtt ttatttggaa
     7981 atgctgtatt taatggagta aatacattac ttacagttgg tggatttgta attgtttttt
     8041 cagtagtatt taaaatacta tctcttttta atgtaatctc attaatagca tcagttatat
     8101 atttgcctct ttcattttta ggtgttagta aagaactctg tcaagcattt gtaagtggtc
     8161 tatttgaaat tactatagga tgcaataaag tatctagtat aacaagttct ccagaaattt
     8221 taagagcatc tttagctagc tttttgatag gatttagtgg attatccatc ctagctcaat
     8281 gttgtacatt tctatcaaaa acggacatag atttaaaatt atacatacta agtaaatttg
     8341 cgcatggagt tttagctgct atctttatat ttatacttta tccaattgca aactctgctg
     8401 tgttggtttc tagctttgct gatacataca acgttatata taataattta atctggtttt
     8461 attatcttag ctattatgat acaattcttc aaattgtagt aataatttat ctaatctcag
     8521 cagtatttat tgcaaaaaaa catatgaaaa ctttatctac aaatgggaaa ctaatgcgat
     8581 ataaaaatac tttttttatt aagaaatttt cacgaaaaaa ataaaactaa taaaacttaa
     8641 ctaaatactt ccattttttt attatatttg tcataatttt aataaccttt ttaattagtt
     8701 taacagcata gttatttcat cacaatttaa taattttatt agaaatattt ttcaaaaaat
     8761 tctccttaat cacatatatc acacttgtat aagtttactg ggaaattttt ccattatata
     8821 atgtttcatt tatattatat aataaatacc gacctaaact taccaaatca aactaagagg
     8881 agtggttgaa aatggcaaca agacctatag aaatagacga atataaaaag ataatggaac
     8941 tattacatac tggctttact tatagtgaaa atggcgttga aaaaagattt agaaaaaatc
     9001 ctaaagttgc attggcacta atgttagaag ctaatttagg tctaagaata tcagatattt
     9061 taagattaaa gataggtaat tttaaaggta atatgttgga aataaatgaa aaaaagacgg
     9121 gcaaattaca gtatagacca gttaataaaa atataattga gtccataaat gaacatgcaa
     9181 gaaagtataa tcttaaatca aatgactact tagtaaatat aaagacaaag gctattcaaa
     9241 aacagttgag gataatttgt aaacacttaa atctgtataa tataagtaca catagcttta
     9301 gaaagctcta tgctactact caatttgaaa agagtaacaa taatttagag ttggttaaag
     9361 aacttttaaa tcacagttct gtagccacta cacagcgata tataagagta actcagcaag
     9421 ctattaatga agctagtgaa aatttcttta ttggttaatt ataacgtata catttttcta
     9481 ttatcttatt aattcaccag atattaaaaa agagtgtctc aaaatgaact ttttagttca
     9541 tgagacactc tttttttata aaatcaaata tatttttatt atttcaatac tggaaaaaag
     9601 gcttgtctct ctattttgag acaccctatt aaattttcat aattcttgca taaacatatt
     9661 aatctatctt caataatttt ttagctgatt cactgtaatt atttcttttt acaatttcca
     9721 aaactctatc aatctcatgt tctgactttg cacttacaaa aatcgccaat tcttcatcat
     9781 ctataatatc ataactatct acatatttat ctaaacattc taaaattcca tctttattat
     9841 tatacccacc aaatctaact actgcactac actcatcttc tataagtcca cttaaatcca
     9901 atttcccttt tgaaaaagac tttatatttg aacttctatt atctattata tctataatat
     9961 ctgcataaac agcacttcct gttgcacctc ttccagctcc cttacctaca aaagaaagtt
    10021 cacctactga atcacctact aaaataacta cgttgaactc attatctatc tttgaaagca
    10081 tattattatt gtccactaat gctggtctaa caaatccact tacttctcct gattcatact
    10141 tacttctagc aaccaatttt atcttacaat ttaatttttt tgcatattct atatcttttt
    10201 catctattga agttattcct tctaataata aatctttcca atatactatt tttccataag
    10261 ataaagtaga caatatagat aactttctag cactatcata tcctaaaaca tctgcatctg
    10321 gattaacttc tgcaaatccc aactcttgtg cctgttttaa agcaacatca tatggtaaat
    10381 tttcatcata catctttgat aatataaaat tacaagtccc atttaaaata gcgtttatgc
    10441 tctttatatt gttgccttcc agtgattcta tcaatggttt taaaactgga ataccacctg
    10501 ccacagatgc ttcaaacttg atgctaacat tatttaagtt agctaattct ataaggtcac
    10561 tacctttttc agccagtaaa tctttattag ctgtcactac atgtattttt ttatttaacg
    10621 ctcttgttac ataatcaaaa gctggattta atcctccaat tacttcaact agtatatcaa
    10681 tatccttgtc aaatatatct tctatatcag tagttatctt atcaaaatac ttcttatact
    10741 tgtgtttttc tttattttta actaaaattc caacaacttc tatatctctt tttgaagtat
    10801 tattttctat taaatcaatt aatccacttc ctactgttcc ataccctaat attgctatct
    10861 tcactgtaac atacccccat cattttgatt atattaaatt atttatatat ataaatatac
    10921 caataaaaaa agcttgagta taaactcaag ccatatgacg ggtttatact catctttcag
    10981 cataaatgct gcaggattta gcaccaatgt ataattaaat atactggttg ccgggcttca
    11041 cagggccagt ccctctgcca ctcttgataa gattgctaat taaaatttat gtttaatagt
    11101 ataatacaat ttcttccaat tgtcactatt tttttaactt atttccctaa aaggatataa
    11161 actattatct aacagctctt ttagttggtt tacccttttt tgtaacatta ttatttttag
    11221 tctttgcttt cgcctgttca tgtcctggaa aaagtatttt aggctcttca atattcctct
    11281 tgtaaatagt aaatttaaaa ccaatagcct gcacaaattc agctcttaaa gcttcacata
    11341 ctgcattagc agtttcttta gtatcaagac ctgcattttc taatatagta acttttacta
    11401 attctctagt tcttaacata ccatctaatt gctctaagaa actctctgta acaccttctt
    11461 taccaatttg tgtagtaggc tttaaagtat ttgctaaaga tcttaaatac gccctttgtt
    11521 ttccttttaa catattctca cctctataat tctatactta aattatctat aatctatatt
    11581 taaattaatt gtaaaactca aattctaatt cataaatttt aaccacatct ccatcttcta
    11641 ttcccatttc tctaagccta tcaaaaacac cttgactctc catagctttt tggaaatatt
    11701 gaagggattc catatcatca aaatttacag aatacattat tcttctaagt gctttacctg
    11761 taactacata cacaccatct tcaatatcta tacttaactc ttcttcagta ccaatatcaa
    11821 gttctggtct atacatttct tcttctgaaa ctagctccac atcttctact tcttttagca
    11881 ttttagaaac ataggctatt acttcatcta tgccttgacg agttgctgct gacattttaa
    11941 atactgtata tcctctgcct tctaattcac tcttaaactt ctcaaactta ctctcatctt
    12001 ctaatatatc aaatttattt gcaactacaa cttgtggcct ctttgaaagc ttttcattgt
    12061 acaatttaag ctcatcattt atcttgtcaa aatcttcaat agggtctctt ccctctaatc
    12121 cagatatatc tacaatatgt atcaatacct ttgttctctc aacatgtctt aagaactcat
    12181 gacctaatcc tatgccttca gaagcaccct ctataattcc aggtatatct gcaagtacaa
    12241 aactatctcc aaacttagtt tgaacaacac ctagatttgg tgtaagtgtt gtaaaatgat
    12301 agtttgcaat tttgggttta gctttagtta caactgatag aaatgttgat ttacctacat
    12361 ttggaaatcc taaaagacca acatctgcta tcattttaag ttcaagagtt atccatcttt
    12421 cttctccatc tgtaccagat tttgcaaaag ctggagcttg tcttactgca tttgcaaagt
    12481 gttggttacc tttaccacct ataccacctt ttgcaactat agctgtatct ccttcttttt
    12541 ttaaatctgc taatacaaga cctgtagcct catctcttat tactgtacct gctggtactt
    12601 ttaatactaa attttctcca tttttaccag ctctttttcc tttgcttccg tcgccaccat
    12661 tttgagcttg atattttttc tggtacttaa agtccattag agttctaagc cccaaatcaa
    12721 cttcaaatat gatgcttgct ccacgaccac catctccacc gtctggacca ccagctggaa
    12781 cgtatttttc tcttctaaat gctaccgaac catttccacc atttccagcc ttaacaaata
    12841 ttcttgcctt atctataaac aaattcttca cctacttaat taaaaacttt taattagcat
    12901 taccaaaaat accattataa attcatcagt attataagaa aaaggagtgc ctcaacactc
    12961 cttttatgta tattactcag ctattgctac tggatatata ctaacttttt ttctcttctt
    13021 gtcttttctt tcgaatttaa cagtaccatc aactaatgca aataaagtat catcactacc
    13081 ttttccaaca ttagttcctg gatgtatttt agttcctctt tgtctaacta ttatacttcc
    13141 agcagttact aattgaccat cgaatctctt aacacctaat cttttagata tagaatctct
    13201 accattttta gaagaaccta cccctttttt agatgctaat aattgtaaat tcatgtttaa
    13261 catatggagt caccccctac ttttttaaaa tttttatatt ttttgggtat tcccgtttaa
    13321 tttctttaac cgctaattga aagtgattta gtaataattg tgcgtctttt acatgcgtat
    13381 ctttgactat acactctata tgaccttctt ctcctactac tttttcaaat tcaatctttt
    13441 gtaatttgtc taaagaattt ataactgcaa tagcattaga tgtaactgca gcacatacta
    13501 tatcatagcc tatttcagca aaatcagcat gtccctttaa acaaaaacct tgcaacaaga
    13561 aatcatcatt ataataatat ttaactttta tcataactta accaataact aagcgtttat
    13621 tttttctaca actatcttag tgtatgattg acgatgtcct tgttttcttc tgtaatcttt
    13681 ttttgcttta tacttgaaaa ctataacttt cttagcttta ccttgttcaa caactttagc
    13741 ttgaacagat gctccttcta ctactggaga acctaatttt aattctccat ctttagagca
    13801 agctaatact tcgtttaaag taacaacatc acctgcgtta gcttctaatt tttcaacgaa
    13861 taatacatca ccttcagaaa ctttatattg ctttccacca gtctttacta tagcgtacat
    13921 taaatacacc tcctcggcct caaactcgcc agtacaaggt acttcatttg aagatttaaa
    13981 cctttactgt gcggcattac aattattaat tataaaatat ttacatttta ttgtcaacta
    14041 ttttatcgaa aataacattg gttttattgt gctttaatgt actatttgcg tttaaagaaa
    14101 tctttatatt atatttttca ccaattttat caattatatc catataatat tcttttattt
    14161 gttcaaaaat aaaatcatta aattctacca ttatgttttt gtaaacagta tgttcactta
    14221 ttctcataat ttctttttca atactatcta gtatataatg tacagatttt attgtttgtt
    14281 caccatcaca acatggacaa gacattaaat aatacttatc aatggattct ttttctctac
    14341 gcctagcaac ttctactaaa ccaagttttg tcatacctaa tacttcagct tttcttttat
    14401 ctttattaaa ttcttcttct aggatgtgta aaatctcctt ttgatgcttc tttttatgca
    14461 tatcaataaa atcgattatt attatcccag ctatgtctct aattataagc tgtcttacaa
    14521 tttcttttgc agcttcaaga ttagttttat aaacggtttc atcaagttta ccagttccaa
    14581 taaacttacc cgtatttaca tctataaccg taagagcttc tgttttttca attattaaat
    14641 aacctccact ctttaaccat acttttttat ttaataattt ttctatttta gattctattc
    14701 tgtataaatc aaatacatct ctattttcct caagacacag tttatcaaca taagtcttat
    14761 ctatacctct taaaatagac ttaagttcag agtacttatc ataactattg gtaattatag
    14821 attcaatatc atcattcaca ttatctttta catatttagt tgcaaaatct aaagacttat
    14881 ataataattt cggacctata ccaagtttat attcttttaa aatattttca tactttaatt
    14941 tcaactcttc tatatctttc tttatttcat catgagtgca accttgagcc tcagtcctta
    15001 ttattaatcc tagattttcc ttatttactg ccttagtaat tttttttaat ctaaatcgtt
    15061 ctttttcatc agtaatttta tttgatattg tagttctatc attggaaggt atatagacaa
    15121 tataccttcc agaaatacta atctcagtgt ttaattttgc gcctttagtt cctatttctt
    15181 ctttatttat ctgaaccaaa atatcctgac cagattttat agtacttccc ttttttaatt
    15241 gtaaataagc aagtttctta aatccaatat ctacaaaaca agcttctatt cctggtataa
    15301 ctttttttac gattccacga tatatattgg atacagtctt cttatttaaa ttgtcttcaa
    15361 caaaaagttc tgttaatctt tcatcttcaa gtactgctgt tttctgggac cctattaggg
    15421 actcaataac tatctttttc acaacttcac ctctcgagct tacataggag tcactaattc
    15481 cccatcttct aatacatata aatctcttct caatatatca acatctaatg ggtcaatatc
    15541 aagttcaaac atctctaata tctttggtat aagtatatta gtatttaaat tagtttttga
    15601 gcctgtagct atagtagcag taagagtaat atgttcatct tctaaattta aaacatcaaa
    15661 ggttcttatc ataggtctta tatctacttc aaccatctta ccttttttat tcttttttgt
    15721 tatgattatt tctttattat tcataaagtc aattactttt ctttttacaa actctttcgt
    15781 cataggtctt ttcaaatcta tattaaacaa atattctcca tagtctatca cagaagaaag
    15841 agaaggtgtt tgtctatcaa tttcttttgc tttgataaaa tctatcccat ctggaagttg
    15901 aattgatact ttatttaaaa attcctctac tgataaatca tcttcttcta tttcaatatc
    15961 tacatactca ccttgacttt ctgtccctaa agccaaagca ttgccataac tcattttagg
    16021 gtgaggatta aatccttgag aatgtgataa gtttatttca gctcttctaa acgctctttg
    16081 taacaatctc tgaaggtcaa gatgtgatat atatatcatg tcgccttcct ttttaaattt
    16141 tactcttatt atcttactca tattagcaaa gcccccttcc tatatcatgt atatttattc
    16201 cacaaccatt gcagttatgt ctacaatcag aagtgatttt ttctttttta gcattttcgt
    16261 cttctctaat taagaatttt ttactaatac ctacatctat atggtcccaa ggaaatacct
    16321 cttcatagtt tctatttcta tgtgcataaa aatctataga aagattattc ttttccatag
    16381 cttctttcca tatatctaaa ttaaaatgtt ctgcccatcc atcaaattta gcacctagtc
    16441 taaatgcatc atatataact tttcctattc ttctatcgcc tctagctact actgcttcca
    16501 tcaaactagt ctttgaatca tgataattgt attttatatt gttatttctt aatttattta
    16561 ctaaatgtct ctgcttatta ataacttctt ctgtagtatc ttgaccatgc cattggaaag
    16621 gtgtaaatgg ctttggtaca aatgtagaag tactcactgt aacactaaaa cttctcttta
    16681 gctttcctcc atttactttt ctataaatat caataacttt ataagctaat ttagctatac
    16741 catctaaatc ttcataagtc tcagttggta aaccaatcat aaaatacaac ttaacactat
    16801 tccaccccat ctcaaaagct ctttctgttg cattttccaa atcttcttct gtaacacctt
    16861 tatttataac atctctaagt ctttgagttc ctgcttctgg agcaaaagta agtccagatt
    16921 ttcttacttg ttgtatttta tcagcaattt ccattgaaaa attatctaat ctaagggatg
    16981 gaagcgaaat cccaatatta ttagatgcat attcatctac caaataatca gtcaactctg
    17041 ataacttact ataatcactc gtactaagtg atgataatga tatttcatca tatccagtat
    17101 tcttaactaa tttatctaat atttctttaa gtcttttaac actcttttct cttattggcc
    17161 tgtatatcat acctgcttga cagaatctac atcctctagt acatcctcta aataattcta
    17221 gtactattct atcatgaaca gtatctatat aaggtactat tagtttttct ggataatcta
    17281 cagtctcaac atcttttatt atccttttag taggattttt agtaatccct tctctattag
    17341 gagttacact ttgaactgta ccatcttcat tgtacttgac atcataaaaa cttggtatgt
    17401 atactccttc tatgctagat attttatata aaaaatcttc tctagtcgtt ttatttttct
    17461 tccattcttt atactcattt actacttcta aattaacctc ttcaccttca cctaatatta
    17521 ctacatctat aaaatctgct aatggttcgg aattataagc acaaggacca ccaaccatta
    17581 taaatgggtc ttccaaagtt ctttcttctt ttaatattgg tatattggct aaatctaata
    17641 tatttaagat attagtgtat gaaagttcat attgaagtgt aaaagtgaca aaatcaaagt
    17701 ttgttatagg ctctcttgac tctaatgcaa ataatggtat acttttttct ctcattatat
    17761 tttccatatc aactgcagga gaatacactc tctcacaaaa tacatcttca tccttattta
    17821 ttacatcata taatatatga ctccctaagt gactcatacc aacttcatat aaatctggaa
    17881 agcaatgtgc atatcttatc aattcactat tactagtatc tttatgaatt gaatttattt
    17941 catttccaag atatctagca ggcttttcta ctttttttaa gatttttttc aagtctactt
    18001 tattcattta ttaaaattcc tccaaacaag ctctttattt ttattcttat ttataagtag
    18061 tattatacac gataaaatat tttctatcaa aaatataact aaaatattga atttaaaata
    18121 catattattc acaaaataga atagctaaaa atatttaaga atattttcta ttaaattttt
    18181 attgaaatac aattttttta caaagtatga tatattataa acataaaact attgttttac
    18241 atgacttatc atgacatctt ttgtttcatt cacctcaaaa ttgggtatgt tacttatata
    18301 atgataaatt ttaggagggt taaaaatgag taaagttttt gaagtaaaaa aagatatcta
    18361 ctttactggc gtagtagatg aaggtttaaa agtattcgac ataataatgg aaactgagtt
    18421 tgggacaact tacaactctt atttaatcaa ggacgaaaaa acggttttat ttgacactgt
    18481 aaaggcaaat tttaaagatg agtttttaag taatttatca gaggttactg atatagctaa
    18541 aattgactat gttgtaattc atcacacaga accagaccat gcaggtagtt taaaatactt
    18601 attagatata aatcctaata tagaagttta ttgtactaaa gctgctaaac tatacttaga
    18661 tggtcaaata aatagacctt ttaactgtca tgtaataaaa gatggtgaaa ttttaaatat
    18721 cggtaaaaga aatcttagat ttataactgc tccattctta cattgggttg atacaatgtt
    18781 tacttacatt gaggaggaca agactctttt aacttgtgat gcatttggtt gtcactttgc
    18841 aagtgttgat gcagaggtag tgaatagtga agattatcta aaatcagcta aacactacta
    18901 tgattgtata gttaagccat ttgctaaaca tgttttaagt gctgtagata aggtggtagg
    18961 actaaacata gaatttgata ctatattgac ttctcatggt ccaatgctta ctaaagaccc
    19021 aatggctgct gttaaaagat atgttgagtg gtctacagaa gctgttaata caactaatca
    19081 aaatcaagtt tctatattct acttatcagc ttatagtaat actttagaga tggctaaaaa
    19141 gataaaagaa ggacttgata aagaaggtgc aaaggctgaa ttatatgatt tagaagatat
    19201 gacattaaca gaaatgcacg atacattagt agtgtcaaaa gtaatattac taggttctcc
    19261 aactataaac aaaactatgg ttaaacctat gtgggattta ttctctgtta tagacccaat
    19321 ggctaaccaa ggcaagatag caggagtatt tggttcattt ggttggagtg gtgaaggtat
    19381 aactatggct gagactttac ttaaatctat gtcattcaaa atgcctgtag aatctcttaa
    19441 gaaaaaattc ttccctagtg aagagacatt aaaagagtgt atggcatttg gtgcagaatt
    19501 tgcaaaatta gttaaatagc cattaatcac acataatatt atttaaaata aatgatttta
    19561 tatttaaaaa taaattaaaa tggagttgtc tcaaaattaa tttttctaat ctaattttaa
    19621 gttcaactcc ttttttattt cccaaaatga ctaatataat gataaatgca ccctcaccaa
    19681 gagggtacat tatttataat cttttctatt tgtatacttg tatatttata ttaattcaca
    19741 tatctagcat atcttacttg ccctcttcca gaattaggtt ctatttcacc aatttctatt
    19801 ttaataatta cattattgtt tttacacttt tttgataatt ctgctgcaga aaggttttta
    19861 ccagtaaaat ctaaagaaaa tggcttatca tattcaaaag gatactcaac aggtacacca
    19921 tctggagcta atgcaaccat accatatggt gtattcataa accctattac agaaactgta
    19981 tgccttgatg ggtctaaatt tgaatttttg cttgtaaact taattgtatt tattgatgag
    20041 ttgattaaac tacacggaac tccctcatac ctttcaattt tgggacgtag attatttcca
    20101 taaacttcat caagtattgc ttcttcttga tctgctatca tagtgtgatt cccaggtcca
    20161 actggcgcag aaggcttagc tggcgtaggt ttcttaacag ttggcgttgg attctgtgtc
    20221 tttactgatt caccaacagc tggataggct ttcttgtcaa atggtaatgg gtgagtaaga
    20281 ctcttgtata tgatgtctgc tcctgctgat ttgttggcat caatatctac atgatacaaa
    20341 cgtccatcct caaaacgaat aagattccac tgatggtcat ttgttgtact atgtacataa
    20401 taacattcaa ttcccatagc atcacataaa gctttcaagc tcctacacat tccagaacaa
    20461 gcaccttttt tagtaaccaa tgtataagca gtattggctc cctctgctgc gttccagtta
    20521 acatatgttg tgttgttttt aatgtgatta taagcaaccc taatcttagc atctgtggtc
    20581 atgttctttg taattttctt gttcataaag tctgctgttg cttgtgctac cgaattaagt
    20641 tgagcttgtg ataactttgg accatatata ctgttggttc cagctacata gcttttcggt
    20701 gtctttgcag taacatcttt agctgtcata ccataaactg ttacactcat acatgaggtt
    20761 aaaaccatcg ttgcaatcaa tagtttagta gctaatttcc tgattttttt catattagca
    20821 ttctccttta taatatattt tactttcaat tccctaccat taattaagtt cattttagat
    20881 ttaaaatatg gattatcaac atgtgtgcat agcaattcac gtggtcatag aatatttaga
    20941 tttcttggaa aatctgttct atatttccta tatagttcgt acacaatcca actcacaaac
    21001 ctgcattttt gatatcatca caataaaatg ataataatta atatattcaa aaatgttggt
    21061 ctgtaacagg tttgtcaatt ttctaattat tggtaatgac gagagtgagg agaattgtca
    21121 ataatacatt taaaattaaa attgtataca cttcatacat tctcccctaa caacattata
    21181 cagattataa tttgtatatt caatacattt tttggaaggt ataattatat caaaatatgt
    21241 gattatgtat agatacaatc cactatggag aaatataaaa gaaaagagta taacaaacaa
    21301 taaaaaataa gatgattcaa attttatttt gaaacatctt atttactcta caaattaaaa
    21361 ttatttggtg aattatttaa ttattatttt ttattaaaga aattttaaat cttaatataa
    21421 aatacgctat tatacataat aatccaaatg caaatgctac tggaattaac atctccgatt
    21481 caaaataaaa gaaatattct aagaaaatat ctggatttac tatattccca ttcttgtcta
    21541 aaaaatcttt tccattatat cctaaatgct gtataaaatt acttatgtta ttataatctg
    21601 cttccctctc atataccata ttagcattca atccagaaga acttaaatta gtgtttccat
    21661 ctttcatatc tattacatat agtctaagag ctttgacttt actatactta tctttatcaa
    21721 gtattttttc aagttctaac ttatattcac caatatttgt catatcatgt ctttctgcca
    21781 attgctttat ttctccatca agttcttttc ctaaattata catattatgt tgtaaactat
    21841 tattgtaata ccacataaat ccagaaatta aaaatgcaat tatacttatg taacctaata
    21901 cttttttaaa ccccattaca attgatttat gcctatccaa tgataaagat agttctttta
    21961 ttatattaca taattcatac tgcatttcat caccatcatc aaatctttca attgcttttt
    22021 tacatgcttc ttcttcatct aatccttcta atttaaattc atttgcagat tctataagat
    22081 ggcatctcat ttcctgtttt aattctatag tacttttatt gtcacatttt ttatatagtt
    22141 tatctaaata ttcatctatt agtttcataa attaatccac ctctcccaaa aattgattca
    22201 taaccttttt aataaatctc cattcttgtt tctttatttc taaaaaatct ttaccttcat
    22261 tagttaaatt ataatatttt cttcttccac ctgaactttg ttcattgcta taataagatt
    22321 taattaaatt tttagattcc attcttttaa gtgcaagata cattgttcct tcttttaatt
    22381 caaactcact tctttctctt acttgctttg caatttcata tccataacaa tctttctttt
    22441 ctaatataga aacaattaat atatcgatat aaccttttaa cacttcttta tttaactgca
    22501 ttatgtacca ccgccttttc acctactact ctataatgta tagtacttat atttactata
    22561 ata
//
LOCUS       DAELSY010000061        21402 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.061, whole genome shotgun sequence.
ACCESSION   DAELSY010000061 DAELSY010000000
VERSION     DAELSY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.061"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..759
                     /gene="tuf"
                     /locus_tag="KPG09_001981"
     CDS             <1..759
                     /gene="tuf"
                     /locus_tag="KPG09_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBG0966591.1"
                     /translation="ELLELVEMEVRDLLTEYDFPGDDTPIVRGSALMALEDPKSEWGD
                     KIVELFEQIDEYIPAPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLKVQDEVEL
                     VGLTEAPRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAH
                     TKFTAEVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTME
                     VDLINSIVVEEGLRFSIREGGRTVASGVVATIIE"
     gene            1132..1443
                     /gene="rpsJ"
                     /locus_tag="KPG09_001982"
     CDS             1132..1443
                     /gene="rpsJ"
                     /locus_tag="KPG09_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBG0966592.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            1535..2164
                     /gene="rplC"
                     /locus_tag="KPG09_001983"
     CDS             1535..2164
                     /gene="rplC"
                     /locus_tag="KPG09_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBG0966593.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            2194..2817
                     /gene="rplD"
                     /locus_tag="KPG09_001984"
     CDS             2194..2817
                     /gene="rplD"
                     /locus_tag="KPG09_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBG0966594.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2817..3107
                     /gene="rplW"
                     /locus_tag="KPG09_001985"
     CDS             2817..3107
                     /gene="rplW"
                     /locus_tag="KPG09_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBG0966595.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTASFKKAVVKLTADSKEIEFFQGM"
     gene            3137..3967
                     /gene="rplB"
                     /locus_tag="KPG09_001986"
     CDS             3137..3967
                     /gene="rplB"
                     /locus_tag="KPG09_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBG0966596.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            4002..4283
                     /gene="rpsS"
                     /locus_tag="KPG09_001987"
     CDS             4002..4283
                     /gene="rpsS"
                     /locus_tag="KPG09_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBG0966597.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            4315..4650
                     /gene="rplV"
                     /locus_tag="KPG09_001988"
     CDS             4315..4650
                     /gene="rplV"
                     /locus_tag="KPG09_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBG0966598.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            4673..5488
                     /gene="rpsC"
                     /locus_tag="KPG09_001989"
     CDS             4673..5488
                     /gene="rpsC"
                     /locus_tag="KPG09_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBG0966599.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            5524..5955
                     /gene="rplP"
                     /locus_tag="KPG09_001990"
     CDS             5524..5955
                     /gene="rplP"
                     /locus_tag="KPG09_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBG0966600.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5957..6160
                     /gene="rpmC"
                     /locus_tag="KPG09_001991"
     CDS             5957..6160
                     /gene="rpmC"
                     /locus_tag="KPG09_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBG0966601.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            6184..6438
                     /gene="rpsQ"
                     /locus_tag="KPG09_001992"
     CDS             6184..6438
                     /gene="rpsQ"
                     /locus_tag="KPG09_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBG0966602.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            6464..6832
                     /gene="rplN"
                     /locus_tag="KPG09_001993"
     CDS             6464..6832
                     /gene="rplN"
                     /locus_tag="KPG09_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBG0966603.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6856..7161
                     /gene="rplX"
                     /locus_tag="KPG09_001994"
     CDS             6856..7161
                     /gene="rplX"
                     /locus_tag="KPG09_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBG0966604.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            7191..7733
                     /gene="rplE"
                     /locus_tag="KPG09_001995"
     CDS             7191..7733
                     /gene="rplE"
                     /locus_tag="KPG09_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBG0966605.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            7751..7936
                     /locus_tag="KPG09_001996"
     CDS             7751..7936
                     /locus_tag="KPG09_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBG0966606.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7969..8367
                     /gene="rpsH"
                     /locus_tag="KPG09_001997"
     CDS             7969..8367
                     /gene="rpsH"
                     /locus_tag="KPG09_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBG0966607.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            8398..8940
                     /gene="rplF"
                     /locus_tag="KPG09_001998"
     CDS             8398..8940
                     /gene="rplF"
                     /locus_tag="KPG09_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBG0966608.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8976..9344
                     /gene="rplR"
                     /locus_tag="KPG09_001999"
     CDS             8976..9344
                     /gene="rplR"
                     /locus_tag="KPG09_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBG0966609.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            9365..9874
                     /gene="rpsE"
                     /locus_tag="KPG09_002000"
     CDS             9365..9874
                     /gene="rpsE"
                     /locus_tag="KPG09_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBG0966610.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9889..10074
                     /gene="rpmD"
                     /locus_tag="KPG09_002001"
     CDS             9889..10074
                     /gene="rpmD"
                     /locus_tag="KPG09_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBG0966611.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            10107..10550
                     /gene="rplO"
                     /locus_tag="KPG09_002002"
     CDS             10107..10550
                     /gene="rplO"
                     /locus_tag="KPG09_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBG0966612.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            10594..11862
                     /gene="secY"
                     /locus_tag="KPG09_002003"
     CDS             10594..11862
                     /gene="secY"
                     /locus_tag="KPG09_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBG0966613.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11879..12529
                     /locus_tag="KPG09_002004"
     CDS             11879..12529
                     /locus_tag="KPG09_002004"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBG0966614.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            12530..13276
                     /gene="map"
                     /locus_tag="KPG09_002005"
     CDS             12530..13276
                     /gene="map"
                     /locus_tag="KPG09_002005"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBG0966615.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            13298..13567
                     /locus_tag="KPG09_002006"
     CDS             13298..13567
                     /locus_tag="KPG09_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HBG0966616.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            13605..13823
                     /gene="infA"
                     /locus_tag="KPG09_002007"
     CDS             13605..13823
                     /gene="infA"
                     /locus_tag="KPG09_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBG0966617.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13859..13972
                     /gene="rpmJ"
                     /locus_tag="KPG09_002008"
     CDS             13859..13972
                     /gene="rpmJ"
                     /locus_tag="KPG09_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBG0966618.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            14125..14496
                     /gene="rpsM"
                     /locus_tag="KPG09_002009"
     CDS             14125..14496
                     /gene="rpsM"
                     /locus_tag="KPG09_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBG0966619.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            14532..14930
                     /gene="rpsK"
                     /locus_tag="KPG09_002010"
     CDS             14532..14930
                     /gene="rpsK"
                     /locus_tag="KPG09_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBG0966620.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14962..15585
                     /gene="rpsD"
                     /locus_tag="KPG09_002011"
     CDS             14962..15585
                     /gene="rpsD"
                     /locus_tag="KPG09_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBG0966621.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            15663..16610
                     /locus_tag="KPG09_002012"
     CDS             15663..16610
                     /locus_tag="KPG09_002012"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBG0966622.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            16631..16972
                     /gene="rplQ"
                     /locus_tag="KPG09_002013"
     CDS             16631..16972
                     /gene="rplQ"
                     /locus_tag="KPG09_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBG0966623.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            17092..17925
                     /locus_tag="KPG09_002014"
     CDS             17092..17925
                     /locus_tag="KPG09_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG0966624.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17913..18779
                     /locus_tag="KPG09_002015"
     CDS             17913..18779
                     /locus_tag="KPG09_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG0966625.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDISFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18773..19576
                     /locus_tag="KPG09_002016"
     CDS             18773..19576
                     /locus_tag="KPG09_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBG0966626.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            19608..20339
                     /gene="truA"
                     /locus_tag="KPG09_002017"
     CDS             19608..20339
                     /gene="truA"
                     /locus_tag="KPG09_002017"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG0966627.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            20459..20890
                     /gene="rplM"
                     /locus_tag="KPG09_002018"
     CDS             20459..20890
                     /gene="rplM"
                     /locus_tag="KPG09_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBG0966628.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20919..21311
                     /gene="rpsI"
                     /locus_tag="KPG09_002019"
     CDS             20919..21311
                     /gene="rpsI"
                     /locus_tag="KPG09_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBG0966629.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8554 a         2533 c         4506 g         5809 t
ORIGIN      
        1 gagttattag agttagtaga gatggaagta agagatttat taacagaata tgatttccca
       61 ggagatgaca ctccaatagt aagaggttca gcattaatgg cattagaaga tccaaagagc
      121 gagtggggag ataagatagt agaattattc gagcaaatag atgagtatat accagctcca
      181 gagagagata cagataagcc attcttaatg ccagtagagg atgtattctc aatcacagga
      241 agaggaacag ttgcaacagg aagagtggaa agaggagtac taaaagtaca agacgaagta
      301 gaattagtag gattaacaga agcaccaaga aaagtagtag taacaggagt agagatgttc
      361 agaaaattat tagaccaagc acaagcaggg gataatatag gagcattatt aagaggagta
      421 caaagaaacg agatagaaag aggacaagta ctagcaaaga ctggatcagt aaaggcacac
      481 acaaagttta cagcagaagt atatgtactt aaaaaagaag agggtggaag acatacacca
      541 ttctttgatg gatatagacc acaattctat ttcagaacaa cagacgtaac aggagcttgt
      601 aagttaccag aaggaataga gatggtaatg cctggagata acgtaacaat ggaagtagac
      661 ttaataaact caatagttgt agaagaggga ttaagattct caataagaga aggtggaaga
      721 acagtagctt caggagttgt tgctacaata atagagtaat tctttactaa actaatggat
      781 ttaatataaa aaaaagaggg gggtaactcc tctctttttt gttttttgtt gacaagtagc
      841 gctaaagcat atatactaaa ctagtgcctt gagacagatt aacaatcaag tttaaggtta
      901 tgatgttgta atagaaaata aaattgaaaa aaacagaaaa aaaacttgaa aaaatgtaac
      961 agatgatata aaatataata gtgtgcttga gagattaaga aaataataat taaatcctag
     1021 tgaaatacga actgatatcg atttaaaagt tacaacacac ccggagcagt gtatcggtat
     1081 tggttgtacg caacttgaaa attaaacacg tgataaagga gggaaaacaa aatggctaag
     1141 aatgaaaaaa taagaataag attaaaatca tatgatcaca aattattaga tttttcagca
     1201 ggtaaaatag ttgaaactgc taaaaaggct ggatcacaag tttcaggacc tgtgcctcta
     1261 ccaacagaaa aacaagttgt aactatatta agagctgtac ataagtacaa agactctaga
     1321 gaacaatttg aaataagaac tcataaaaga ctaatcgaca tagctaatcc aacacctaag
     1381 acagttgact cattaatgag attagactta ccagcaggtg tagatataga aataaaatta
     1441 taagaacccc taagatatta tcctatagat gattatctta gaatgattac tctaaaagat
     1501 gtaatccgct gtaagaatta taggaggtgc taatatgaaa ggaatattag gcaaaaaagt
     1561 tggtatgact caaatattca ctgataaagg tgtagttata cctgtaacag ctgttgaagc
     1621 agggcctatg gtcgttactc aaataaaaac agttgataaa gatggataca atgctataca
     1681 aataggtttt gaagatgcta aagaaaaagc tttaaacaaa cctaaaaaag gacatttagc
     1741 tgctgctaat gttttaaaga aacatttaaa agaatttaga gtagattctg tagaaggata
     1801 cacagtagga caagaaataa aagctgatgt atttgaagca ggtgcaaaaa tagatgttac
     1861 tggaataagt aaaggtaagg gattccaagg tccaataaaa agacatggac aaagtagagg
     1921 tcctgaaact catggttcta gataccacag aagaccaggt tcaatgggag cttgttctta
     1981 cccaggtaga gtatttaaaa acaaaaaatt agctggacat atgggaagcg taaaggtaac
     2041 agttcaaaac ttagaggttg ttaaagtaga tgctgacaag aaccttatat tagttaaagg
     2101 agctatacca ggagctaaag gttcagtagt aactataaaa gaagctataa aggtttctaa
     2161 ataatgacta acctagagaa aggaggaata acaatgccaa aattaaatgt actaaatgtt
     2221 agtggacaaa acgttggaga aatagaatta tcagattcta tatttggcgt agaagttaat
     2281 ggacatgttt tatacgaagt tgtaaaaaat caattagcaa ataagagaca aggaactcaa
     2341 tctgctaaaa ctagagcaga agttagaggt ggcggaagaa aaccttggaa acaaaaagga
     2401 acaggtagag caagacaagg ttctacaaga tctgtacaat gggtaggtgg aggagttgct
     2461 tttgcaccta aaccaagaag ctacaaatat acattaccta agaaggtaag aagattagct
     2521 atgaagagtg ctttatcttc aaaagttcaa aatagtgaag ttatagtatt agatgcatta
     2581 aacatggatg ctcctaagac taaagaattt gctcaaatat taaacaatat aaatgctgct
     2641 aaaaaagctt tagtagttat agctgacaaa aacgataacg taataaaatc agctagaaat
     2701 atagaaggtg ttcaaactgc attagttaat actatgaatg tatatgatat attaaaatac
     2761 gattcattta taataactac agatgctgtt aaaaaagtgg aggaggtgta cgcataatga
     2821 ctaatccaca tgatgtaata ataagaccag ttgtaactga gcacagtatg gctgaaatgg
     2881 gtgaaaaaaa atacactttt gttgtggcta aagatgcgaa caaaactgaa ataaaaaaag
     2941 cagtagaaaa agtattcgga gttagtgttg ataaagtaaa tactttaaac tacgatggaa
     3001 aagttaaaag aatgggtaga actcaaggaa gaacagcaag ctttaagaaa gcagtagtta
     3061 aattaactgc tgatagtaaa gaaatagaat tcttccaagg aatgtagtat tataacaaac
     3121 gaaggaggga aaacagatgg ctataaaaaa gtttagacca acttctcctg ccttaagaca
     3181 aatgacagtt ttagtttctg atgaaataac atgtaatcaa ccagaaaaat ctcttttagt
     3241 taacttaaag aaaaatgctg gtagaaacgt acatggtaga ataactgttc gtcatagagg
     3301 tggaggacaa aaaagaaaat atagaataat agattttaaa agagataaag atggtatacc
     3361 tgctaaggtt gctactatag aatatgatcc aaacagaact gcaaatatcg cattattaaa
     3421 ctatgctgat ggagaaaaaa gatatatatt agctccagtt ggaattaacg ttggagatac
     3481 tatattatca ggattaggtg ctgatataaa accaggaaac tgtttagcat taaaagatat
     3541 gccagttggt actatcattc ataatataga attaaagcca ggaaaaggag ctcaattagt
     3601 tagatctgct ggagtttctg cacaattaat ggctaaagaa ggaaaaaatg ctttattaag
     3661 attaccatca ggtgagatga gattagtaag cataaactgt aaagctacta taggtcaagt
     3721 tggaaatata gagcatggta acgtagttat cggtaaagca ggtagaaaaa gacatatggg
     3781 aataagacct actgtaagag gttctgtaat gaaccctaat gaccatccac acggtggagg
     3841 ggaaggtaga tctccaatag gtagaccatc tcctgttact ccatggggta aaccagctct
     3901 tggatacaaa actagaaaga aaaataaagc ttcaaataaa ctaatagtat caagaagaac
     3961 taagtagtaa atttattttt gctcggaaag gaggaatata aatgtcaaga tcaactaaaa
     4021 aagggccttt tgtccatgca agacttttaa agaagataga agctatgaat gctagtggaa
     4081 ataaagaagt tataaaaact tggtcaagat cttcaactgt atttccacaa atggtagaaa
     4141 acactatagc tgttcatgat ggaagaaaac atgttccagt atatataaca gaagatatgg
     4201 ttggacataa attaggtgag ttcgttccta ctagaacttt caaaggacat aaggatgacg
     4261 aaaaatctaa taagagaaaa taattaaatt atgactaaga aaggaggaat aacaatggaa
     4321 gcaaaagcta ctgctaaata cgtacgtgta tcaccaagaa aagcaggcca aatatgtgac
     4381 cttgttagag gaaagaatgt tgatgaagca ttagcaatat taaagtttac tccaagagga
     4441 gcagcttcaa taatagctaa ggttgtaaaa tcagctaaag ctaacgcaga aaacaatcac
     4501 gaaatggata ctgaaaaatt atatatagca tcaatagttg ctaatcaagg accaacaatg
     4561 aagagattca tgcctagagc tatgggtcgt gcaactacaa taagaaaaag aacttctcat
     4621 atagaggttg ttgttaaaga aaaaaaataa ttaatagata ggagggaaag taatgggtca
     4681 aaaggttaat ccacacggct taagggtcgg tgttataaaa gattgggact caagatggtt
     4741 cgctactgat aaaaaagagt tcggaaactt attattagaa gaccataata tacgtaaatt
     4801 cttaaagaaa agattatact cagctggagt tgctaagata gaaatagaaa gatcagctaa
     4861 caaaataaaa atggacttac acgttgctaa gcctggtgta gttataggaa gagctggtgc
     4921 aggaatcgaa gcattaaaag ctgaattaga aaaaatgaca aagaaaacta taatagttaa
     4981 tatagtagaa gtaagaagta cagataaaaa tgctcaatta gtagctgaaa atatagcttt
     5041 agctatagag agaagggttg ctttcagaag agcaatgaaa caagctatac aaagagcaat
     5101 gaaatctggt gctaaaggga taaaagtttc tgcttcagga agattaggtg gagctgaaat
     5161 ggctagaact gaaggttata gtgaaggaaa tgtgccacta caaactttaa gagcagatat
     5221 agattatggt ttcgctgaag ctgatacaac ttatggaaaa atcggtataa aagtttggat
     5281 atgtaatggt gaagttttac caactagaga cggtgtaaat ccaagagaag aaagcagaaa
     5341 gagcgataga agagacaaca agagagataa tagaagaaac gatagaagag gaaatgacag
     5401 aagaggaaat gataacagag gaaactacag aggacaaaga cctcaaggtg gatcaagacc
     5461 tcaaagaact gaaaacaaag gaaattaata atagttaggt tcaaggaagg aggaaaaata
     5521 ctcatgttaa tgccaaaaag agtaaagcgt cgtagagttc atagaggaag catggctggg
     5581 caagctcata agggtaataa agttacttat ggtgagtttg gattagtagc attagaagct
     5641 tcttggataa cttctaatca aatagaagct gccagaatcg cgatgactag atatataaaa
     5701 agagggggaa aggtttggat aaaaatattc cctcataaac cagtaacaag aaaaccagca
     5761 gagactcgta tgggtgcagg gaaaggttct ccagaatatt gggtagctgt agttaagcca
     5821 ggaagagtta tgtttgaatt agcaggtgtt tctgaagata aagcaagaga agctatgaga
     5881 cttgctgcac ataaattacc aatcaaatgt aaatttgtta aaaaagaaga tttagaagta
     5941 aagggtggtg aataggatga aagctaaaga attaagagat ttaacaagcg aagagctaat
     6001 gaacaaatta aacgacttca aaagtgaatt atttagctta agattccaat tagctactgg
     6061 tcaattagaa aatacagcta gaataaagtt tgttaagaag gatatagcaa aagttaaaac
     6121 tgttcttgct gaaagaaagt tatacgaaac tagagcttaa tttggaaagg aggcttttta
     6181 aacatggaaa gaggaagaag aaaagttaga ataggccgtg ttgttagtga taagatggat
     6241 aaaacaatag tagtagctgt tgaagaattc gtacgtcatc cactatataa taaacgtgtt
     6301 aagagaacta agaaattcaa ggctcatgat gaaaagaata tatgtaacat cggagataga
     6361 gttaaaataa tggaaactag accattatct aaagataaga gattcagact agttgaagtt
     6421 gttgagaaag ttaagtagtt tttgaagaag gagggattac gatatgatac aacaagaatc
     6481 acgtctaaga gttgctgata actcaggagc taaagaactt ttatgtatac gtgttctagg
     6541 cggaagtaaa agaagatatg gtaacatagg cgacgttata gttgctactg ttaaaagtgc
     6601 aacacctggt ggagttgtaa aaaaaggtaa ggttgttaaa gctgttatag taagaagcaa
     6661 acaaggcgta agacgtaatg acggaagcta tatatctttt gatgaaaatg ctgctgttat
     6721 aataaaggac gataaaactc cagtaggaac tcgtatattc gggcctgttg ctagagagtt
     6781 aagagacaat gaattcatga aaatagtttc tcttgctcca gaagtactat aattaaggag
     6841 gtgcaatagg acatgatgcg tgttaaaaaa ggtgacactg ttgtagttat agcaggtaaa
     6901 gataaaggta aaaaaggttc agttttaaag gtatatccta aaactagcaa agtgttagtt
     6961 gaaggtgtaa atgtaataac taaacatcaa aaaccaagtg ctatgaacca acaaggtgga
     7021 ataataaata aagaagcccc aatacacata tctaatgtaa tgccatttga tcctgaaaca
     7081 ggaaaaggcg ttagagtaag atatgaagta aaagatggga acaaagtaag agtatcagca
     7141 aagagcggaa aagaattata ataaatattg cttgaaagga gggaccttaa atggcttcta
     7201 gattacaaga aaaatacatg aaagaagttg ctcctgcttt aatggagaaa tttggataca
     7261 aaaacgtaat ggagatacct aagttaaata aaatagttat taacatgggt ataggtgacg
     7321 ctagagaaaa tccaaaagga ttagaaaaag gcgttgaaga attagaaatg atatcaggac
     7381 aaaagcctgt tataactaaa gctagaaaat ctgttgctaa cttcaaatta agagaaggaa
     7441 tgccaatagg aacaaaagtt acattaagag ctgacaaaat gttctacttt atggacaaat
     7501 tagtttcagt ttctttacca agagttagag attttagagg agttaaccct aatgcttttg
     7561 atggtagagg aaattatgct ttaggagtta aagaacaatt aatattccct gaaatagaat
     7621 atgataaaat agataaagta agaggaatgg atataatatt tgttactaca gcaaaaactg
     7681 acgaagaagc tcgtgaatta ttaaaattat taggaatgcc attttctaag taagcaaagg
     7741 agggattccc gtggctagaa aagcgatggt tgttaagcaa caaagaaagc aaaagtacgc
     7801 tactagagaa tacactagat gtactatatg cggaagaccg cattcagttt tgaaaaaatt
     7861 tggtatatgc cgtatatgct ttagagaatt agcttataaa ggtcaaatac ctggtgttag
     7921 aaaagcaagc tggtagaagc ttgattagaa tggaaggagg gcaacaaaat gacaatgaca
     7981 gatccaattg cagatatgct tactcgtata agaaatgcta atgttgttaa gcatgaaact
     8041 gtagatgttc cagcttctaa tatgaagaaa gaattagcta gaatcttatt agaagaaggt
     8101 ttcataagag gttatgatgt tatagaagat ggaaaacaag gtatcataag aatacaatta
     8161 aagtacggac aagaaggcga aagagttata acaggtctta agaaaatatc taagcctgga
     8221 atgagagttt acgctgctaa ccatgagata ccaaaagtat taaacggatt aggaatatca
     8281 gttatatcta cttcaaaagg aatattaact gacaagcaag ctagaaaaga aaatgttggt
     8341 ggagaagtaa tctgctacgt ttggtaatta atatgaacaa tgtaaggagg tgcgaccatg
     8401 tcaagaatag gtgttaagcc aataataata ccagcaggtg ttgaagttac tatagctgaa
     8461 ggaaatttag ttacagtaaa aggtccaaaa ggaactttaa ctaaacaatt aagtgctgag
     8521 ttaaacataa aaaaagaaga aaatacaata atggttgaaa gaccaactga taataaaaaa
     8581 catagatctt tacacggact aactagaact ttattagata atatggttgt aggtgtaaat
     8641 actggtttcg aaaagaaatt agaattaaaa ggtgttggat atagagctca aaaacaagga
     8701 aagaaattag ttatgaactt aggtttctct catccagttg agatggaaga tccagaagga
     8761 ataactgttg aagctccaaa ccaaacagag ttaatagtaa aaggtataga caagcaatta
     8821 gtagggaatt atgctgctaa gataagagct tggagaaagc cagagccata caaaggtaaa
     8881 ggtataaaat acgttgatga agtaatcaga cgtaaagaag gaaaaactgg taaaaaataa
     8941 taccaaaact taaacgctag aaaggagtga gctaggtgtt aaaaaaggct gataaaaatg
     9001 ctaatagact tcaaagacat aagagagttc gtagaaaaat aagcggtact tctcaaagac
     9061 caagattatg tgtatttaga agtgctaaca atatatatgc tcaaataata gatgacacaa
     9121 aaagagtaac tctagttgct gcatcttctt tagaagcaga agttaagagt gctgtcaatc
     9181 atacaggaaa caaggaagca gctaagaaag ttggagaatt agttgctaaa aaagctgttg
     9241 aaaaaggaat cactgaagtt gtatttgaca gaggtggata cttatatcat ggaagaattc
     9301 aagaattagc tgaaggtgct agagaagctg gtcttaagtt ctaatacgaa ggagggaaaa
     9361 atacatgcta cgtcgtaagc cgatagatgc aggacaactt gacttacaag aaaaagttgt
     9421 tgaagtaaga cgtgttacta aggttgtaaa aggtggtaga aatttcagat ttgcagcatt
     9481 agtagttgtt ggagatgaaa acggacacgt tggtataggt gctgggaaag caatggaagt
     9541 accagacgct ataaaaaaag cagttgaaga tgctaagaaa aatctaatag tagtgccaat
     9601 agttgggact acaattcctc acgaagtacg cggacacttt ggtgctggaa atatattaat
     9661 aatgcctgct gtagaaggta caggagtaat agctggggga cctgctagag ccgtacttga
     9721 gctagctgga ctaaaagacg ttagagctaa atctttaggg tctaacaatc caagaaatat
     9781 ggtaaatgct acaatagaag gacttaattc tctaaaaaca gttgaagata tagctaaact
     9841 tagaggtaaa aaagttgaag aacttctagg gtaaggaggt agtgtaaaat ggctaaatta
     9901 caaataaagt tagttagaag tgttatagga actactccta accaaaaaaa gaatgttgaa
     9961 gcgttaggat taagaaaaag agaacaagtt gttgtaaaag aagacaatgc tcaaacaaga
    10021 ggtatgataa ataaagtaag tcacttatta gaagtaactg aaatagctga ataattttta
    10081 taaagtagtg caaggaggtg caatccatga agttacatga gttaaaacct gctgaaggtg
    10141 cagtaagagc taagagaaga ttaggtagag gtactgcaac tggacaaggt aaaactgcag
    10201 gacgtggaca aaaaggtcaa tggtctcgtt ctggtggtgg agtaagagta ggatttgaag
    10261 gtggacaaat gccactagct agaagacttc ctaagagagg tttcaataac atatttaaga
    10321 aagtttacac agaagttaat gttgaagttt taaacagatt tgaaaatgga acagaaataa
    10381 ctgcagaatt attaaaatct actaaaacta taagcaaaat aggtaaagac ggaattaaaa
    10441 tcttaggtga aggtaattta gagaaagctt taactgttaa agctgctaaa tttacagctt
    10501 cagctcaaga aaaaatagaa aaagctggtg gaaaagcaga gttagtataa taacctgcta
    10561 atctcctagc cagtacaggc ggggtgaatt agcgtgctgt caaaattaaa acaagcttgg
    10621 aagattaaag cagtaagaaa aaaagtaatg tatacactta tgatgatcgt gatattcaga
    10681 ataggtacta ctataccagt tccaggcata gacactagta ttatacaaaa gatggtaggc
    10741 ggcaatagtc tactttctct gtataatatg tttactggtg gagcatttag taacttttca
    10801 ctatttgctt taggtataag tccatatatc actgcatcaa tcataataca acttctaaca
    10861 gtaggttttg aaagtttagc agaacttcaa aaatctggtg aagaaggaaa gaaaaaaatc
    10921 aataagtaca ctaaatatac agcactagct ctagcagttg tacaagcttt gggaattaca
    10981 ttaggaattg taagaagtgc tttaatatca aatagtgtgt tttttatcac aactgttgta
    11041 ataacattag tttcagcaag tatgttagta atgtggattg gggacaaaat tactgaaaaa
    11101 ggaatcggta atggtagttc agtaataata tttgcaggta taatatcaag aatacctaca
    11161 gatgttatta aaatatcgca acaagttaaa tctggcgaag ttgcaccttg ggttatagtg
    11221 attctagctg tagttatatt acttacagta acaggagtta catttataca agaagcaact
    11281 agaaagatac ctgtacaata tgctaaaaga gttgttggaa gaaaaatgta tggaggacaa
    11341 agttctcata taccaatgaa agttaaccag tcaggagtta tgcctataat ttttgctagt
    11401 tcacttttag ctttccctca aacaatagct atgtttatgg gtccaaatgc tcaggctttt
    11461 gttcaaaaat acttaagtat ggcaacagaa caaggatttt ggacatatag atcaattgag
    11521 attctactta taattttctt ctcatatttt tatacaactg tatcatttaa tacagaagac
    11581 atatctaaaa atatgaaaaa taatggtgga tttataccag gtataagacc aggtgagcct
    11641 actatggatt atttaaatag aattttaact agattaactt tagctggagc tactttctta
    11701 gcaataattg ctatggtgcc agctcttaca acacattata tgaaagtaaa tatgagtcta
    11761 gctgggactt cattgctaat cgttgttgga gttgctttgg agcttaagag acagttagag
    11821 tcaaacttag ttatgagaag ctatcaaggt ttcttaaaat aaaatggaga tggataatat
    11881 gagaataata ttacttggac ctccaggtgc tggtaaaggt actcaggcgg caggaatagt
    11941 agaaaaatac aatatacctc atatatcaac aggagatata ttcagaaaga atataaaaga
    12001 gggaacagaa cttggaaaaa aagctaaaga atacatggac caaggtttat tagtaccaga
    12061 tgagttaact gtaggtttag ttactgatag aatatctcaa gaagattgta aaaatggatt
    12121 tatgttagat ggatttccaa gaaatgtagc acaaggagaa catttagata tcttcttaaa
    12181 aaatgctggt atatcactag ataaagttgt caatattgaa gttgataaga gtatattagt
    12241 gtctagagca gttggtagaa gaatatgtaa gtcttgtgga gctacttacc atgttgagtt
    12301 taatcctcct aaagtagaag gtgtatgtga tgtatgccaa ggagaattat atcaaagagc
    12361 tgatgataat gaagaaactg tatctaagag aatacaagtt tatctagatg aaactaagcc
    12421 tttagtagat tattatagca aacaaggtat aatagcagat ataaaaggtg atcaagcaat
    12481 agataaagta tttgaagata ttgtcgcagc cttaggaagt ggaaaataaa tgattatctt
    12541 gaaatcaaaa aaagaaatag aacttttaag agaagcaggt aaaatagttg ctgacactca
    12601 tgaagtttta agaaaagcta tttctccggg aatatctact ttagaattag ataaaatagc
    12661 tgaagagaat ataagaaaat acaatgcaga gccttctttt aaaggatatg gaggttttcc
    12721 aggaagtata tgtgcttcta tcaatagaga ggtagtacat ggtattccag gagaaactat
    12781 attacaagaa ggtgacattg taagtttgga tataggagcc tattataaag gatatcatgg
    12841 tgattctgcg aaaactcacg gagtaggcat gatatctgaa gaagatagga aattgataga
    12901 agtaactaga gaaagcttct atgaaggaat aaaatttgct aaattaggat atagactttc
    12961 agatatctca cacgcagtac aaacacacgt tgagaaacat ggtttctcgg tagttagaga
    13021 tttagtgggt catggagttg gagcaaatct tcatgaagac ccacaagtac ctaattatgg
    13081 actcccagga aaaggaccaa gacttagaga aggaatggtt attgctatag agccaatggt
    13141 taatgcaggt cgttatcatg taaagactct atcagatggg tggacgactg ttacaataga
    13201 tggaaaaaaa tcagctcatt atgagcatac gatagcaatt actgaacatg aacctttgat
    13261 attgacaaag ttgtaattta aaaagtaggt gaaaaaagtg ctatcagata atttaagtat
    13321 aggtcaagtt gttaaagttt cattaggacg agataaagga aatttattct ttgtagtcaa
    13381 aataattaat aatgaatatg tattaatagc tgatggaaaa aagagaaaac ttgacaaacc
    13441 taagttaaag aaagtgaagc acttaaaaaa atataatttt attaatgatg aagttagaaa
    13501 aagagtagta tctggacaag agattacaga ttctttttta agagctgaac ttactaagtt
    13561 aaattagata atttaactta ttgaaatgga gggctggtta gttaatggcc aaaaaagatg
    13621 ttatagaatt agaaggtaca gtttcagaag ctttacctaa tgctatgttc aaagttaaac
    13681 tagaaaatgg acatgagata ttatgccaca tttctggaaa gctaagaatg aacttcataa
    13741 gaattcttga aggtgacaag gtgaatgttg aactttctcc atatgacctt acaagaggaa
    13801 gaattacttg gcgtaagaag tagactatat taatatttag tctaaggagg gattaataat
    13861 gaaggttaga ccatcagtaa aaccaatatg tgaaaaatgt aaagtaataa aaagaaaagg
    13921 aaaagtaatg gttatctgtg aaaatccaaa gcataagcaa aagcaaggat aagaaattat
    13981 tactagcatt ttgttaaatt ttatagtata atactatatt gtgatgttaa caaaagaacg
    14041 ttaaaatcgt tgaacaatct ggcgtaggat tggcatatga tgtgaagttt taaacactaa
    14101 aaactatgtg taggaggtgc gatcatggca agaatagctg gggtagattt acctagagaa
    14161 aaaagagcgg agataggctt aacttatata tatggtatag gtaaagcaac tgctaacgaa
    14221 atattagcta aggctgagat aaatccagat actagaataa aagatttatc agaagatcaa
    14281 gttaatgagt taagaaaagt tatagatgac gatttcttag ttgaaggtga cttaagaaga
    14341 gaaattgctt taaacataaa gagattaaga gatataaaat gttatagagg aataagacac
    14401 gctaaaggtc ttccattaag aggacaaaga actaagacta atgctagaac tagaaaaggt
    14461 cctagaaaaa cagtatctcg taaaaagaaa aaataaataa ataataacta gatgaaggag
    14521 ggaaaaggaa aatggctaaa ccaaaaaaga aagttacacg tattagaaga agagaacgta
    14581 aaaatataga acgtggtcat gctcatatac aatcaacttt caataataca ataataactt
    14641 taactgacgt tcacggaaat gctatatctt gggcaagttc tggacaatta ggattcaaag
    14701 gatcaagaaa atcaactcca tttgcatctc aaatggctgc tgaaacagct gcaaaagctg
    14761 cgatggaaca cggactaaaa agtgttgagg tattcgtaaa ggggccaggt tcaggaagag
    14821 aagctgcaat aagagcttta caagcaactg gactagaagt aactatgata aaagacgtta
    14881 ctccaatccc acataacgga tgcagaccac caaagagaag aagagtgtaa ttaataagag
    14941 tacaatagga ggtgtagata aatggcaaga tatacaggtg catcatgtag acaatgccgt
    15001 agagagggaa tgaaattatt ccttaaaggt gatagatgtt atacagacaa atgtgctata
    15061 gtaaaaagaa actatgctcc aggtcaacat ggccaaggaa gaaagaaagt ttctaactat
    15121 ggattacaat taagagaaaa acaaaaagtt aaaagaatat atggagtttt agagactcaa
    15181 tttagaaatt tatatgaacg tgctgaaaat atgcctggta aagcaggtga gaacttatta
    15241 agtttattag agagaagatt agataatgta gtttacagaa tgggactagc atcttctaga
    15301 aaagaagcta gacaattagt gactcatggt catttcactt taaatggaaa taaagtagac
    15361 ataccttcat taatagttaa ggttggagat gttatagaag ttaaagagaa atcaagatct
    15421 tctgcaaaat tcaaaaattt agtagaagtt aactcaagaa tagctcctaa gtggttagaa
    15481 gctaatgtag aaggaatgac agcaaaagta gttggtgttc caacaagaga agatatagat
    15541 cttgagatag cagagcactt aatcatagaa ctttactcta aataataagc acacttttaa
    15601 aaattatttt aaaattttgt ttaccctcag tggattactg aattttaagg agggttttgt
    15661 ccatgataga aatagaaaag ccaaaagtag atatagttga gcttagcgaa gactatagat
    15721 atggtaagtt tgttatagag cctctagaaa gaggatatgg aataactata ggtaacgcat
    15781 taagaagaat attattatca tcattaccag gtgtagcagt gaatgctata aaaatagatg
    15841 gagttcttca tgaattttca acaatacctg gtgttaaaga agatgttact gagataatat
    15901 taactttaaa agagctttca gcaactatag atggtgaagg aagtagaaca cttaaaatag
    15961 aagctcaagg accatgctct atcacaggtg cagatataat ctgtcctcca gatgttgaaa
    16021 tattaagtaa agatttagca atagctacat tagatgataa tgctaaactt aatatggaga
    16081 tatttgtaga taaaggtaga ggttatgttt ctgctgaaga aaataaaaca gagaatgttc
    16141 caataggtgt tttacctgtg gattcaatat atactcctgt tgagaaggtt agttatcatg
    16201 tggaaaacac aagagttggt caaaaaacag attatgataa attagtacta gaagtttgga
    16261 ctaatggtag tataaatcct caagagggaa tatcattagc tgcaaaggta ttagttgagc
    16321 atttaaactt atttatagac ttaactgaac atgtaagtag tgttgaaatc atggtagaaa
    16381 aagaagaaga tcaaaaagaa aaagttcttg aaatgactat agaagaatta gatttatcag
    16441 ttagatcata taactgttta aagagagcgg gaattaatac agttgaagaa ttagctaata
    16501 aatctgagga tgatatgatg aaggttagaa acctaggtaa gaagtcatta gaagaggtaa
    16561 tacaaaaact agaagaactt ggattgggat taaaaccaag tgaagaatag cgagcaaagg
    16621 agggataaga atggctaagt accgtaaatt aggacgtgaa acagctcaca gaaaccttat
    16681 gttaagaaac ttagtaactt gcttactaag aagtggaaga atagaaacta cagtgactag
    16741 agcgaaagaa actcgtagaa tggctgaaaa gatgataact cttgctaaga gaggagatct
    16801 tcatgctaga agacaagttt tagcttatgt tatggatgaa acagtagtta ataacttatt
    16861 cacagattta gctccaaaat atgctgagag aaatggtgga tacactagaa taataaaaat
    16921 agggccaaga aaaggcgatg ctgctgaaat ggcttttata gaattagtat agtaaggaaa
    16981 ggattaggct tgatggctta atcttttttt tatttttttg actaaggtat gatataataa
    17041 tgtgtaaaat gatttttata agttattgct tactaaaagg agtatgagtt aatggataat
    17101 atagtaaagg taaataatat ttcgtttgag tatattacag atgaagcaaa acttaaagca
    17161 atagataatt taagcctaga tgttaaaaaa ggagaatttg ttgcgataat aggacataat
    17221 ggttctggta agtctacttt atctaaaaac ttaaatgcta ttcttatgcc tactgaagga
    17281 aatattctta ttgatgatat ggatactaaa gaagaagaaa gattatggga tataagacaa
    17341 actgcaggca tggtatttca aaatccagat aatcaaatag ttgctactat agtagaagaa
    17401 gacgttgcat ttggacctga gaacttaggt atagaaccaa aagaaataag acgaattgta
    17461 gaggagtctt taaaaagtgt aggtatgtat gatttaagag atagacagcc tcatttgtta
    17521 tcaggaggtc aaaagcaaag agttgcaata gcaggaatta ttgctatgag gcctaaatgt
    17581 ataatatttg atgaagcaac agcaatgtta gatccatctg gaagaaaaga agttatgaaa
    17641 acaataaaaa gacttaataa ggaagaaaat ataactgtta tacatataac gcactttatg
    17701 gaagaagcag tagaagctga tagagttgta gttatggaaa aaggaaagaa aatcttagag
    17761 ggaactccta gagaagtatt cagtaaaata aagatgttaa aggaaatcgg tttagatgta
    17821 ccttgtatga cagagttatc tagtttatta atagaagaag gtataaatat tagtagtgat
    17881 atattaactg tggatgagat ggtgatggaa ttatgtcaat tatagtaaag aatttaacac
    17941 atatatataa tgaagggatg ccttttgcaa gtaaggcact tgatgatatt tcttttgaga
    18001 ttaaagatag agattttgtt ggtcttatag gtcatacagg ctcaggaaaa tctacattaa
    18061 tacaacattt aaatggatta ctaaagcctt cctcaggaga aatatttata aatgatttta
    18121 atataacaga taaaaacttg aatttaactg agattagaaa aagagtaggg gttgtatttc
    18181 aatatccaga ataccaattg tttgaagaaa cgattgataa agatatagct tttggaccct
    18241 ctaatttagg cttagaggag tctgagatac ataacagggt aaaagcatca atggaagctg
    18301 taggacttga ttatgaggga tttaaagaca aatctccttt tgaattatct gggggacaaa
    18361 agcgtagagt agcaattgct ggagttatag ccatgaaccc agaagttctc atattggatg
    18421 aaccaactgc tggtcttgac cctggtggaa gagatgaaat ttttaactta ataaaagact
    18481 tacatgaaaa aaagaatatg actataatac tatcatcaca tagtatggat gatatggcaa
    18541 agttagcaaa aactttaatt gttatgaatc atggtagcgt agaatttatg ggaactccta
    18601 gagaagtgtt taaatcaaat gctagtaaat taaaagatat aggattagat attccacaag
    18661 ttttagagct tgcattgaaa ttgagagaaa aaggctttga catcagtgag gatatattga
    18721 ctttagaaga agcaaaacag gaaatattaa aagttgtgag aggacgagga ttatgttaaa
    18781 agatataact atagggcaat actacccaac aagttctgct attcataaat tagatccaag
    18841 gataaagctt gtagcgacaa ttgtatttat ggtatctata tttgtagtaa ataaattttg
    18901 gccttatata gtagttttat tatgtttatt agctatgata aaattggcta atataccagt
    18961 caaatatatt gttaagggtg ttaaacctct taaatggatt attttgttta catttcttat
    19021 aaatattttt ttcttaccag gtgatgaaat atggtcattt ggatttttag ctattacaaa
    19081 acagggtcta agacaagcaa tttttatggc aataagatta atatttttag tagttggtac
    19141 atcattgctt actttaacaa cttcaccaat agaattaact gatggaatag agagattgtt
    19201 gaatccattt aagaaaattg gtttgccagt tcatgaactt gcaatgatga tgacaattgc
    19261 tttacgtttt attcctacat tgttagatga gacagacaaa ataatgaagg ctcagatgtc
    19321 tagaggtgca gattttgaaa gtaagaatct tataaataga gcaaagaatt tagtaccact
    19381 tttggtacct ttatttgtaa gtgcatttag aagagctgat gaattggcta tggccatgga
    19441 ggccagatgt tacagaggtg gacataatag gactaagatg agagagtctg ttatatcaag
    19501 aagggattat atggcatgtg tatttcaggt tgtttatctg ggagcaatta tagctactag
    19561 atttatagca atttaattaa taagttatag atataatgag gtaaagtatg agaaatataa
    19621 aaattaaaat ccaatacaat ggtaagaatt attgtggatg gcaaaagcaa cctgattctt
    19681 tagggataca aggtacaatt gaaagggcta tatatgatat aacaaaagaa gaaaccagtc
    19741 ttatagggtc tggtagaact gattcaggag ttcatgctat tggtcagatt gcaaatttta
    19801 aaattaattc aggtataagt atagaaagta taccaatggc actaaatgcc aagttgccaa
    19861 aagacatatc agttatagaa gcatgtgaag taaatgatga ttttcactcc agatacagtg
    19921 caaaaggtaa aacatataaa tatttagtat ataatagcaa gtttagaaat ccaatactta
    19981 gtgagatatc atatcaagta aaatatgaac ttgattttga taaaatgtgt tcagaagcaa
    20041 aaagtttatt aggaacacat gattttaaag gttttatgag ttctggttca tctgtaaaag
    20101 atacagttag aacgatatat gatattgata taagtaaaaa agatgacttg attacttttg
    20161 agattagtgg taatggtttt ttatataaca tggtgaggat tattgttgga acacttgttg
    20221 atatgggtcg tggcagaatt aacgaaccat ttttagatat aatacaatca aaaactaggt
    20281 caagatgtgg acatacagct ccagcacaag gattatttct caaaaaagtt cattattaac
    20341 ttgacacacc aggctacatg tattaaaata tatgagaatg tgttaataag tccacttgcc
    20401 ccggacttga cataaacggt aaaaaaattt tatgaaaagt agttaaggag ggacagttat
    20461 gaaaagttat atagctaagc cagctgatgt acaaagaaaa tggtacttag ttgatgctga
    20521 aggaaaaaca ttaggtcgtt tagcaacaga aattgcgaca gtattgagag gtaaacataa
    20581 accaacattt actcctcacg ttgatggtgg agattttgtt gttgtagtaa atgcagaaaa
    20641 aatagtttta agtggtaaga aattagatca aaaatattac agataccata ctggttatgt
    20701 aggtggatta aaagaaatat cttacagaga tatgatggat aagaaaccag aagaagttat
    20761 atcacatgct gtaagcggta tgttacctaa aaataaatta agaagcagaa tgatgactag
    20821 attaagagta tttgcaggtg ctgagcacac tcatgcagct caaaatccag aagttttaaa
    20881 ctttaaataa gattaatagg tgaaaggagg agtttataat ggctaacgtt caatattatg
    20941 gaactggaag aagaaaaagt tctgttgcta gagtaagact agttgcaggt gaaggaaata
    21001 tattagtaaa tggaagagca ttagaaaatt attttaatta tgagacatta ataagagatg
    21061 ttaaacaacc attagtttta actggtaatg aaaataaata tgatgttata gtaaaagttg
    21121 aaggtggagg attcactgga caagctggag ctataagaca tggaatatct agagctttat
    21181 taaaagctga tttagattta agacctgctt taaagaaaga aggtttctta actagagatg
    21241 caagaatgaa ggaaagaaag aaatacggat taaaagcagc aagaagagct ccacaattct
    21301 caaaaagata attacttatt tttttggaaa agcaaaatcc tttatttact gttattcaga
    21361 aagtattggt attttaaaaa tgttgggtta agttaattat tg
//
LOCUS       DAELSY010000062        21360 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.062, whole genome shotgun sequence.
ACCESSION   DAELSY010000062 DAELSY010000000
VERSION     DAELSY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21360
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.062"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(206..466)
                     /locus_tag="KPG09_002020"
     CDS             complement(206..466)
                     /locus_tag="KPG09_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBG0966630.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCMLPHKKDNYNYIMGKFQDLVKITSKK"
     gene            683..1966
                     /gene="hflX"
                     /locus_tag="KPG09_002021"
     CDS             683..1966
                     /gene="hflX"
                     /locus_tag="KPG09_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG0966631.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTSESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGENGITLDVNFDEEDFNIYREYILEK"
     gene            1974..2651
                     /locus_tag="KPG09_002022"
     CDS             1974..2651
                     /locus_tag="KPG09_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBG0966632.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEAGEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            2737..3180
                     /locus_tag="KPG09_002023"
     CDS             2737..3180
                     /locus_tag="KPG09_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG0966633.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTNCVLAPEFQE
                     GGFYKVKDALDMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            3486..4130
                     /locus_tag="KPG09_002024"
     CDS             3486..4130
                     /locus_tag="KPG09_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBG0966634.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVVVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITNGTGKIILGEEFYLSEQNGEILI"
     gene            complement(4216..4593)
                     /locus_tag="KPG09_002025"
     CDS             complement(4216..4593)
                     /locus_tag="KPG09_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG0966635.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(4593..4979)
                     /locus_tag="KPG09_002026"
     CDS             complement(4593..4979)
                     /locus_tag="KPG09_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG0966636.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            5158..5913
                     /gene="nadE"
                     /locus_tag="KPG09_002027"
     CDS             5158..5913
                     /gene="nadE"
                     /locus_tag="KPG09_002027"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBG0966637.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYRAEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            5996..6715
                     /locus_tag="KPG09_002028"
     CDS             5996..6715
                     /locus_tag="KPG09_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0966638.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(6883..7887)
                     /locus_tag="KPG09_002029"
     CDS             complement(6883..7887)
                     /locus_tag="KPG09_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG0966639.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            8153..9049
                     /locus_tag="KPG09_002030"
     CDS             8153..9049
                     /locus_tag="KPG09_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HBG0966640.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            9187..10449
                     /locus_tag="KPG09_002031"
     CDS             9187..10449
                     /locus_tag="KPG09_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966641.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            10525..11718
                     /locus_tag="KPG09_002032"
     CDS             10525..11718
                     /locus_tag="KPG09_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG0966642.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LKRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            12347..13141
                     /locus_tag="KPG09_002033"
     CDS             12347..13141
                     /locus_tag="KPG09_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG0966643.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            13235..14575
                     /locus_tag="KPG09_002034"
     CDS             13235..14575
                     /locus_tag="KPG09_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG0966644.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            14712..15944
                     /locus_tag="KPG09_002035"
     CDS             14712..15944
                     /locus_tag="KPG09_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG0966645.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            15984..17126
                     /locus_tag="KPG09_002036"
     CDS             15984..17126
                     /locus_tag="KPG09_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG0966646.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGTDIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGIGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASSTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            17137..17931
                     /locus_tag="KPG09_002037"
     CDS             17137..17931
                     /locus_tag="KPG09_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG0966647.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFAPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            17942..18964
                     /locus_tag="KPG09_002038"
     CDS             17942..18964
                     /locus_tag="KPG09_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG0966648.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELEVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEVLPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            19063..20439
                     /locus_tag="KPG09_002039"
     CDS             19063..20439
                     /locus_tag="KPG09_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG0966649.1"
                     /translation="MKEQWYKDIFARILSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKDKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(20771..21115)
                     /locus_tag="KPG09_002040"
     CDS             complement(20771..21115)
                     /locus_tag="KPG09_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966650.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
BASE COUNT         8028 a         2529 c         4098 g         6705 t
ORIGIN      
        1 ttcactcagg aatgatgatt ttaatattaa ttttgttagc aagtatctta ctatttgtaa
       61 tgtatagatt atataaatct ttcaaaataa aataagatag caaaaacgaa ttatattaaa
      121 ggttatacaa taaaaatagc tatgtaataa tatttaaact aagagtagtc gataaatatt
      181 tatgcatagc tatttctaat agaaattact ttttactagt gattttaact aaatcttgaa
      241 atttgcccat aatgtaattg taattatctt ttttgtgagg taacatgcaa ctactgcaat
      301 ctttgatacc tttatctgta tacttaaagt ttccaccaca gttttctcct aaagcataca
      361 aaggacaata gcaaaataga cagttaaact cttctggttt attagtttta tgacaaggga
      421 aaaattcgca atctttgtga ttaaaaaact tataattttc gcccatattt tcctcctaat
      481 taaataaaat ttaaattttt tatactatat aagattttgg tgataatata attatactaa
      541 ataattttat gaatagtcga taatatagta ttattaatca tagtattaat ggtataatta
      601 gtcaacagaa aaataatgta aaaattatgt aagtgtattt tgtaattaga atatgtttta
      661 taacaaaaaa tgaggtgatt aagtgaaaga aattcaagaa aaagcacttc ttgtaggtct
      721 gaatttgact acaatagtga aaaaaaatga tgatatagac actagtgaat ctatggaaga
      781 attaaaagag ctaacaaaag ctgctggggc agaagttgta ggtagcttaa ttcaaaataa
      841 acattctgta gatgcagctt attatatagg aaaaggtaaa gtagaagaaa taagagccta
      901 tagtgactct ttagatgcta ctttagtaat ttttaatgat gaattatctg gtgctcaaat
      961 cagaaatata gagaatgttg ttggaagaaa agtaatagat agaactactt taattttaga
     1021 tatatttgct caaagagcac ttagtaaaga aggtaaatta caagttgaat tagcacaatt
     1081 aaaatataga ttgccacgac tttatggaat gggtggtgag atgagtagaa caggtgcagg
     1141 gattggtact agaggtcctg gagaacaaaa actagaaata gataagagac atatacttaa
     1201 taaagcagca gacataaggc gtgaacttaa agaagtaaaa aagaataggg agacacagag
     1261 agttaagcgt ttaaagtcaa atataccgat tgtagcactt gttgggtata caaatgcagg
     1321 aaaatctact cttttgaacg aattaataaa gacacacaag gattatgaac aagaaaaaga
     1381 agtttttgta aaagatatgc tttttgcaac tttggatgta actctaagaa aggctctttt
     1441 gccgaataaa aaagaatttt tagtagtaga tactgttgga tttgtaagta aacttcccca
     1501 tgatttagta gaggcgttta aagcaacatt ggaagaagta cagtatgcag atttaatttt
     1561 acatgtaatt gatgcgacaa atactagtta tgaattgcaa aaaagtacga ctgaaggtgt
     1621 attaaaagaa cttggtgtaa atgataaaaa acatatactt gtatataata aagtggataa
     1681 gctagaattg gatatatatc ctaaaagtca agaagatatt gtgtatattt ctgcaaaaca
     1741 aggcataaat atggataagc tattaaatat gatagaaatt gctttaatgg aaaatactta
     1801 ttcagtaagc ttgatgttac cttatgagag aggtgatata tttagtagaa taaaagataa
     1861 atataatgtg gagaattttg agtatgggga aaatggcata acgctagatg taaacttcga
     1921 tgaagaagat tttaatatct atagagagta catactagaa aaataggttg aatatggata
     1981 taaattggga aaatatagat aatcttgaag attattttat aacctatctt ctttataaag
     2041 aatctaaaac agtatctcaa attagtaaaa ttagaaatat ttcaagtact gaagcaggtg
     2101 agcaacttat acaagcaaaa ttaaaaataa aggaaatgca aaaagatgat tttgaggcat
     2161 ctaaggatat tttagataaa tttttagaac tagataaaat taagagactt gattttatgg
     2221 atagcttaga tgacgaaaaa atggtatact ttaagagaaa ggttttcaaa agaatccttg
     2281 tggaaaaaaa tgctgaagat ttaattgttt taatttgggc gacaggtgag ctaaaagatg
     2341 atagattctt aaagttgctt catcagctca caaaccacag acactcagat attagaagaa
     2401 taacttattc agcaatcaga aagattgaat caccaagtag tagagaagta ctgcaaaaag
     2461 gtctctacga taaaaatgca caaactagac agtattgtgc aaaggctctt tcaaaactag
     2521 gggatgaaaa cagtttaaag attttacagc agttgaaaga caagaataag aattttgaaa
     2581 aagaatatgt gcttagagct tatgacgaag ccattaagtc attggaaaat gcaaaaaatt
     2641 tgaaaaagta attttttatt aaagcaattt gtttacttaa tgatgtattt ctagagttta
     2701 acaaggtaat aaataaatta atgggagggg tttcaaatga tagtaagacg ttgtgcaggt
     2761 ggagtagtgt tttatgctaa taaagtactt attgtaaaaa atgatagggg agaatggaca
     2821 cttccaaaag gaaagatact tggaggagga cttccatatg agagtgctgt tcaaagagtt
     2881 aaagtagaaa ctggcataga tgctaaaatg atagatgtgg ctggcgatac tatgtatgaa
     2941 tttttctcaa gaagtagaca acaagaagtt tgtaatgcaa taatgtggta tgttatggaa
     3001 gcttgtaaca ctaattgtgt acttgctcct gaatttcaag aaggtggatt ttataaagta
     3061 aaggatgctt tagatatgtt atcacatcat aaagagcaag ctttagtaga agtatcttat
     3121 aaaaagttta aggaattaaa gaaattagtt tcagagggcg atgatgttcc agcaaattaa
     3181 ttgtgaattt agaggattct gaggaaggga tagccccttc cttttttaat tgaagcagtt
     3241 ttaagcgtaa attatatagt ctctttaaat tatgatttat caatttagag ctttagtagt
     3301 ttatatttaa aatcagtaag atatatttaa aataaaaatt aaaaagtata caattttatt
     3361 atataattgt cttatgatat tgattaaaga agtattaaaa gtccatttat ttttaaatag
     3421 aataatttta tagatatata atttgatgtg tatattagaa aatgattaaa ataggaggaa
     3481 atacaatgag caattacagg acattacatg aatttggaac agatgaaata actattgaaa
     3541 agtctgtttt tattggatat gctaagccta taaagagtga agaagaagca ttagagttta
     3601 taaatgaaat aaagaaaaag cataaagatg caactcacaa tgtgtgggct tatactgtag
     3661 gtaaaaatat gaatatacaa agatatagtg atgatggaga accacaagga acagctggaa
     3721 tacctacatt agaggttata aaaaaagaag atttgagaga tgtagtggtt gttgttacta
     3781 ggtattttgg aggaacaaaa ttaggtgcag gcggtctagt tagagcttat acaaaaggtg
     3841 ctaaattggg acttgaagca ggtaagataa tttataaggt aatgtatcaa gaagttaagg
     3901 ttaagataga ctatactcaa cttggaaaag tgcaaaatga gttaatgaat ttggggtatt
     3961 ttataaaaga tactgtatat gaggataatg ttgagatagt agtgtattca agattagaag
     4021 atgtagagaa attatcagaa aaaatgattg atataacaaa tggaacagga aaaataattt
     4081 taggagagga attttatttg tctgaacaaa atggagaaat tttaatataa tctaaaaggt
     4141 tattagaata gttaatacct caaatagcca attagtattt ttaagtgaaa agccattaaa
     4201 ttgatttaat ctaaatcaat ttaatggctt tttatcggat tttgcacaga atcctttttt
     4261 tacttcaaat aaaccgacta aaagtaaaaa tatcccaaat atttttttta atatatctga
     4321 agacagataa ttagctaata gtgaaccaaa tacagctcct aaaaccccaa acacagctat
     4381 aggtataacc aatttaaaaa ttacattttt attttttata tgtatagcaa gtgctataag
     4441 tgtcataggg atagatgaaa gtaaatttac gctttgtgct attttagtgt ctatgccagc
     4501 aagtaaaacg agtgcaggta taaggattgt tcctccaccc attcccattc ctccaataat
     4561 tcctgcaaaa aatccaatta gagcaaataa cattaaaaca ccatccttaa tgctgcaatt
     4621 atcataatta ttccaaaaga aattctaaga aactttccag taaccttatt tagtaattta
     4681 gctcctataa taccaccaag aatacttcca actgcaactt taattgttaa gttaacatca
     4741 taagtacctt tagatacata taaaacagaa cttgctgtcg ttaagaatat tattatagaa
     4801 agagcagttg catgagattt atgttcttct accttaacga tgtcatttaa tattggaact
     4861 aaaagagtac ctcctccaga accaaaaact ccatttataa atcctgtaaa aactccaatt
     4921 atcgtatttt ttaagttaaa atcaaagtat tttttcttta aatcatcagt ttttttcata
     4981 aaaatcacct tctgaaatag ttttaccaaa ataggcaaat tatatttata taaaataaga
     5041 ttaaatataa tctttcatgt ttgtttaatt ttgcataaaa aaaaacatat taaaacaaca
     5101 tattaatata tggtataata tattaaaaca aagtagcaaa tttgagagga gataacaatg
     5161 tcaaatataa aaatacaaat agacaaaact gtagaatggc ttataaataa ggttaatgag
     5221 gcaaatgcaa aaggattaat tgttggtgta tctggaggaa ttgattcggc agtagttgct
     5281 aatttaatta aaaaggcatt tccagaaaac tctatgggag ttataatgag cataaaaagt
     5341 aatcctcaag atagagaaga tgcacttaag gttatagagg gatgtgatat agaatacctt
     5401 gatttagacc ttatagaacc acaaagtgct atattagaca tggtagtagg aaatttaaaa
     5461 gataaacatt tatatagagc agaatattta aagatgacag atgcgaactt aagagcaaga
     5521 gtaagaatga gtacaattta tacaattgct aacaatctag gatatttagt tgttggtaca
     5581 gacaatgctg cagaaataca cacaggatat tttactaaat ttggtgatgg tggagttgat
     5641 attttaccta ttgcaaattt aactaaagga gaagtatatg aatgggcaaa agagcttggt
     5701 gttcatgaag atttaataaa caaagctcct tcagcaggtc tttgggaagg tcaaacagat
     5761 gaagatgaaa tgggaactac ttacaatatg atagatgctg tgttagaagg tagattggat
     5821 gaagttccta aaagagacca agagataata gaaagacttc atagattaag tgaacacaag
     5881 agaaaaacgc ctgcacaacc tcctaagttc taatttacta tttataagat ttgtgataaa
     5941 atttacaagt gatgaaaatt agaataatta attgtacgta ttaaggagtg gaaatatggg
     6001 acgtataggt aatataatta atagaaaagg taaacaagat gctcaaagag caaagatatt
     6061 tactaaacat gctagagcaa tagcagttgc agctaaagaa ggtggagcag acccagagta
     6121 caatgcagcg ttaaaaacag ctatagaaaa agctaaggca gataatatgc caaatgataa
     6181 tatagataga gctatcgcta aaggtgctgg agcaggcgct ggagaagact atgaaactat
     6241 agtttatgaa ggatatggac ctggtggagt tgctgttata gtagaaactt taactgacaa
     6301 caaaaacaga actgctggta acgtaagata ctattttgac aaaaacggag gaaacttagg
     6361 aactagtgga tgtgtgtctt ttatgtttga taaaaaagga caaatattag ttggtttagg
     6421 agatggagtt tcagaagaag aattgatgga tgttgcatta gaagcaggag ctgaagattt
     6481 cataacagaa gaagatggtt atgaaataat aactacacca gaagattttt ctagtgttcg
     6541 tgatgagtta aaagctaaag gatatgagtt tatatctgct gatgtgaaga tgatacctca
     6601 aactacgact gtattgactg aagaatctca tttaaaaatg atgaataaat tagttgatat
     6661 gcttgaagaa gatgatgatg ttcaagatat atatcataac tgggaagtag aataaatagt
     6721 ataagcatat tgaaatttat aagcattgaa tcaattttaa catgtgataa attttataga
     6781 ataagaaatt actaaaatag acatacgcac ctctaaccta tatttgcata ataatatgta
     6841 agtataggta aggggtgttt ttatatttat aggttaagaa atttaaagta aattttgagc
     6901 tggacttgat tgtctataaa taattgatgg gtatataact tgttgctgaa taccatttat
     6961 acgcccagtt actgtatcaa taatcatcct aagactacgt tctgctgtct cttccatttt
     7021 taaatctata actgtcaaag gaggaatact tcttgccatt agtcgtgaag gtccattact
     7081 tgctgcaaat atttgaatat cctctccaat tacaattcca gattcatgaa atgcattcat
     7141 cataccaaga gctacaacat catgattaca aaaaattact ttttttagtg aattttttgc
     7201 aagcattttt tgagcaatat catatccact atctatttta ttagaacaat aatatataga
     7261 atctgataga tgtattccat gttctttaca tgtttttaaa aaagatttac cccttaaagt
     7321 catacatgta aaggaagttg ggttgaatac aacagcaata tcatttttac cattttgaat
     7381 agcatgtaaa gctgctatac gaccagtttc ctcattatca atagatacac ttgaaaaacc
     7441 aggtatttca cgatttacaa caactacagg aattttaggt ttatttttta ctagatattc
     7501 tatatcatca gcattgatac ctgttaaaac aattccatca taataattat ttgcaaaaaa
     7561 gtattcatca tcacatagat gaccaacttt gtaaggtcgt atgatgatat ttatgtttaa
     7621 atgttctaaa gaaattatgt ggtttattgc ttgaagtata ttggttgatg ccaactcaaa
     7681 tccctcatcc tgccaataaa aagctatttt aatattatta tgtttaatag gagtagagcg
     7741 tttatatcca agcttatcag ctgaatttaa tataagctct tgtgtatgta gcggaatcct
     7801 gcgttcattt cctttaccac ttaagacgta cgagacagtt gctgaagaaa gaccacagtc
     7861 ttttgcaata tctttgattg ttgccatgat aaaatcctcc ttaaatagtt gcttaaattt
     7921 gcttatacca ataatcttaa catatattca actaaagaac aatattctac aaggtgcata
     7981 ttttacagga ttagaataaa taaatttaaa aaaacttctt acttatgttg acataattta
     8041 gtttatagga ttataatatg cttaaataag cttattatta actcaaatta agcaaagtta
     8101 agcaggaaca tgcaatgcta gcttgcaatg tgactgtaaa gggggaaaag gtatgagtaa
     8161 ggtaaatata tttgaagttg gtcctagaga tggatttcaa aacctgaaag agtttttaga
     8221 aactgataaa aagcttaaga ttattgatgg tcttattgct tcagggatta aacaacttga
     8281 aattagttca tttgtaagcc caaaagcaat acctcagatg aaagatgcaa aagaagtagc
     8341 aacttattgt gtagaaaaat atcctgatgt gaaactttat gcattagttc caaatttata
     8401 tggtgctaaa gttgcatggg agtgtggtat aagagatata tcctatgtta tatctttgag
     8461 tgaaactcat aataaaaaaa atataaatcg tacccatgaa gagtctattg atgagcttaa
     8521 aaaaataatg aatacttatc cagacatgaa tatatgtatt ggaatggcta ctgtatttgg
     8581 gtgtcctttt gaaggtttac caaaaattga agatgccata agatttgcta aacgcctttg
     8641 ggaaaacggt atcagggaga taaatctagc agatacaata gggattgctg accctaagca
     8701 agtaagagaa actattagtg cattagttga agctttacca gagtgcagat ggcaggtaca
     8761 tattcatgat actagaaata tgggaatggt taatactctt gtggctattg aatctggaat
     8821 tactactgtt cagtctacac ttggaggatt aggaggatgc ccatttgctc ctggagcatc
     8881 tggcaatact gcaactgaag atttagtata tatgcttgaa cgtatggggt atgatacagg
     8941 agttgatttt gttaaacttc ttgaattatc aaagtatcag aagagttttg taaatggagt
     9001 atatagtggt catcatgtaa atataaagtc tgatagttgt aattgttagt tctattgatt
     9061 aatcgattaa aaaaatatgt tgattaaaaa aatatgctga ttggtaaaat agatttattg
     9121 ataaaaaaga taaattataa aatacattat ctaataaaac aaataaaaaa attgaggggg
     9181 attagtatgg atattatagc tttactttta tttttaggag ttattattat tgcatttgta
     9241 cgtaagaata atgcaggtat tttggctcta gctattggtg tcatagctgt tagaattttt
     9301 ggaatggatg ataaagaact ttatggagct ataagcaact caatgtttac tacacttgtt
     9361 ggtataacac tactctttgc aatagtgaac tcaacaggag cattaaagct tgttgcagaa
     9421 aaaattgtag ctgcttctgg taaacgtgtt tggcttatac ctatagcagt ttttatagca
     9481 ggatttgttg ttgctggtgt tggacctggg gcaattccag cacttgcaat tatcccagca
     9541 atggctgttc ctgtagccat aacggttggt tataatcctg taatgcttgc acttataggt
     9601 gaatgtggat taatggcagg tcgtatgact cctattactc cagaaggtca aattataaca
     9661 accgttgcag aaggggttgg aattgaaaat gttatgccta ctatcttagc ctgtcaaact
     9721 ctttctacaa ttgtattttc aatagtattg tttataattt tcaaaggata taaacttaaa
     9781 aaacctataa atgttataga tagtaagcaa atagaaaaat ttaatataaa gcagataatt
     9841 tctctaataa gcatagtagt tatgattgct tttatcataa tttttgatat gaatgttggg
     9901 cttgcagctt ttgcagtttc agctgtactt atttttttca atattgctga tgaaggagaa
     9961 tgtatcaaat caattccatg gagcacgata gtaatggttc ttggagtagg tgcaatgatg
    10021 acaatcgtag atgcagcagg aggtattgac ttgatgagta atgcattatc atcactgatg
    10081 aatagtaaaa cagctactcc aattatgagt atttcagcag gacttatgtc tatggttagt
    10141 tctgctttag cagtagttta tccaactatg atgcctatgt gtgttgatat agctaaggca
    10201 gttggaggag tagacccact agcacttatt gctgcagtcg gtgttggagg ttctcttgct
    10261 ggtgtaagcc cactttctac tggtggggca ttgattttag cagctatggg ttcaagtaag
    10321 aaagacttta ataaggaaga acaaaataag gtttttgtac agcttttcat tatgagtgca
    10381 gttggcttat tggttattgc agtagtatca atattatcat ttaatgcaat aacacattta
    10441 atgaactaat tataataact gaaaataatt cattaaattt aatttttaat attaatcaat
    10501 ataatataat ttggagggga agttatgaaa aaaggagcat taagcaatat aaaggtactt
    10561 gaccttacaa gagttcttgc aggtccatat tgtacaatga tgttggcgga ttttggagct
    10621 gatgttataa aaatagagat gccaagaaaa ggtgatgata caagaggttt tggacctttt
    10681 gtaaatggag agagtttata ctatgcaaat ataaatagaa ataaaaaagg tatcactcta
    10741 aaccttaaat cagaagaagg aaaaaatata tttttagaaa tggtaaaaca tgctgatgtt
    10801 gtagtagaaa attacagacc aggtgtaatg gaaaagttag gtctgggtta tgatgagttg
    10861 gctaaagtta acaatcaaat aatttatgga gctgtatcag gatttggatg ttatggacca
    10921 tattcagaac gtccaggtta tgatattatt gcacaggcaa tgggtggtct tatgagtata
    10981 acagggcaag aaggaggaga accaacgaga gttggtaatg ctatgggtga tgtactaggt
    11041 ggaatgaatt taactattgg tatactcaca gcactcaacg ctagaaaaat aataggaaaa
    11101 ggacaaagag tagatgtttc cctcgttgat tctgttgtat cgtctctcga aactggtata
    11161 cagagatact ttgattcagg acaacctgaa cttatgggta atagatatgc ttcagcctat
    11221 ccttatgact cttttaaggc aagtgatgga agttttgtaa taggctgtgg taatgacaaa
    11281 ctttttgaaa agttatgtaa aaaagttctt tttagagaag accttttaat agatgagaga
    11341 tttaaaacaa atcttaagcg ttgtgaacat tacaaggctc ttaaaccaga aatagaaaaa
    11401 tggtctgtta agcatacaat aaaagaagca gttgacttaa tatcagaggc tggagttcct
    11461 gctgctccta tctataatct tgattgtgtt acaacagatg aacatatagc aaaagcaaga
    11521 gaaatgattg tagatattag tcatcctatt ataggaaata tgaaagtcaa tggaaatcca
    11581 gttaagttga tgacaacaaa agctaaaatt gatatgccag caccatctct aggggaaagt
    11641 aacgaggaga tttataatgg ttggcttggt tttgatagtg ataaaataaa agatttaaaa
    11701 gaaaatggag ttatttgatt aaaaaatagc taaacacact tgtgacttta gtcatgggag
    11761 gttcagttag aataatatca aaaaatatta aatcaatact gtaaatcaaa tatagaattg
    11821 tcagaagcat ttaagatggg aaaagctact tagacattga gtctttgtgg cttttcctat
    11881 tttcattaag aactttatat tatcagaata gatatgtact aaactaaagt attaacaatt
    11941 taaattaata gaaaataaac tacctaatta tgttgaataa agactacttt agtgaaaata
    12001 aacttataga gatgctattt aaattatttt aatggaaata tcaaaaatga gactgcattc
    12061 tcaaaaatga gactgcgttt tttaagtatt ttctcgaaaa tgagacaaaa atattgtttt
    12121 atgagtttta ttgaagaaaa attcttctgt gaattacaat ttaatcaatt ttaaagttga
    12181 aaattattaa aaatttagtg aaatttatgt atatttcgtt tattttatta aaaataacaa
    12241 ttttttacta aaaataaaga acttcaatgg tgattggcat agtacttgct attttatatt
    12301 aacaagaaat gtagaaaaaa taacaatgta taatgggggg aatatgatgg aatctgtaat
    12361 ggaaaatttg aacaatctta aggtggaggt taaggacaaa gtctgtgtta ttactataaa
    12421 tagacctaaa gctttaaatg ctcttaatag tgacactctt agggaactat cacaagttat
    12481 tgatgtagta tcagaaaatg aggcaatatt aggagttatt attactggag aaggtaaagt
    12541 ttttgtagcc ggtgctgata tacgccagat gcaaaactat aaaagtgagg aaggcagaaa
    12601 atatgcaggt tatgctcagg gaatttttga taaaattgaa gctcttgaga aaacagtaat
    12661 agcagcagtt aatggatatg cattaggtgg tggatgtgaa cttgctatga gttgtgatat
    12721 tagaattgca tcagaaaagg ctatatttgg acaacctgaa gttaatttag gagttattcc
    12781 atgttttggt ggaactcaaa gattatcaag acttgttgga actggaatag ctaaagagtt
    12841 aatttttacg gggagacaag taaatgcaga agaagctaaa tctattgggc ttattaataa
    12901 agtggttcca agtgatttac ttttagaaga gtctatgaag atgatgaacc aaatagttga
    12961 aaaagcgcca atagcaataa gatatgcaaa agttgttatt aacaaaggga tagatatgga
    13021 tttaaagaat gctcttgaac tagagaaaga tattgcaggt ttaacttttg ctacaagaga
    13081 taagcaagaa ggaatgaatg catttataga aaagagaaag ccagtttttg aaaataagta
    13141 gctattataa ctaaattgga acaatgtaga atatattcaa aatctaaatt tcgtgaaaaa
    13201 atacatatag tttaatttta aaaggagtta atttatgaat acgggagtaa ttataagttt
    13261 agtagggatt atcatagctc tatctcttct agttgtaatg gttatgaagg gaataaatat
    13321 ttttattata gcaattgtat gttcatcatt agttgctata acaggaagcc taaatgtgta
    13381 tgatgcactt aaagtggatt atatgacagg atttgttgga tttcttcagg caaatttctt
    13441 tattttccta acagggacat taatgggaaa aataatggag attactggtg gagcaaaatc
    13501 tattgctaag atgattgtaa gatggattgg taaggataaa gcactacttt ctattccaat
    13561 agcttgtgga atattagcct atggtggagt aagtgttttc gttgtaagtt ttgcagtgtt
    13621 tccgatagct ttagaaattt ttaaagaagc tgatttgcca cgaagattta taccagcagc
    13681 tttgactttt ggatgctcaa cttttgctat ggttgcacca ggggcaccac aaattcagaa
    13741 tattgttcca gcatcaacat tagggacaga tattatggcc ggagcagtaa atggttttat
    13801 atcatgtgca gtaatgttta tagttggaag taccattttg tacaaaatgg tttcaaaaga
    13861 aaaagccaat ggtggtcatt ttatagcaca tgaatcagat atatttgatg aagcagaagc
    13921 aactaattct aagagagaaa atggtcctaa tggtgtaatt gccttaattc cattgatagt
    13981 gtcaatttta ataattaatg taaaagttaa tggaaaagcg atagtaccaa ttgaggtggg
    14041 agtatttatt ggagctttat tagtatattt attgttaaat aaatatcaag ataatactaa
    14101 aatagttgga catgttggtg atgcatgtaa aactacagta gtagcaatat gtaatacttg
    14161 tgctgtagtt gcatttggtt cagtagtaaa atcagcagtt ggttttgact tcatagttaa
    14221 tgccatgact aatattcctg gtcctcctat tgcaggggct gcagttggaa ctacagttat
    14281 tgcaggtatt tgtgggtcag cttcaggtgg actaggaatt gcagctccac ttcttggacc
    14341 agtattttta gccaagggtg tttccgctgc agcattaact aggactatgg caatttcatc
    14401 agcagcatta gattcacttc cacacaatgg atatattgtt actgtaacaa atggtctgtg
    14461 taaagaaaca cataaggatg cctatatgcc aatattctgg gtaactgttt taactccgtt
    14521 tattggaacc atagtagcag tcctactgtt tacaatgttc ccgggattgc cataagagaa
    14581 aagataaaag gtataaatta tattttgata ttataaaatc aattttcaaa atatatacaa
    14641 taaatttcat gttttaatac tataaatatg tatttaaaat aacaaattga ccatataagg
    14701 aggaattgaa agtgttaaat aaaccattag agggagttaa ggttattgac ctaacgtatt
    14761 ttgtagcagg tccagggact tctaaaatat tagctgattg gggtgctgac gttattaagg
    14821 tagagccaag ctttggagac ccaggaagaa agacaggggc aacaatgaca atgccaatag
    14881 atgattataa taatcctttt tacagcacat ataattcaaa taaaagaggt ttgtctataa
    14941 atcttaagag tgaaactgga attgaaatta tggataagct tttaagtgaa gcaaacgtat
    15001 ttgtttcaag ttatagaaca ggagcattaa aaagactagg tcttgattat gaatcattaa
    15061 gtaaaaagca tccacacctt atctggggtc agataaatgg ttttggcgat tttggacctg
    15121 ctaaggataa tgcaggattt gatacagttg ccttttgggc aagaagtggg gctatgcttg
    15181 atatagcaga aaaagataca agtccaataa atccacctat agcctttgga gatgcaacaa
    15241 cttcttgtag tttatctgga ggaatatgtg cagctttgta ccagcaagct aagactggaa
    15301 aaggacaaaa agtaatggca tctttatttg ggcaagcaat atggaatgca tcatctttat
    15361 tagcatctac acagtttagt gatgagtatc caaaaactag aaaaaatgct atttcaccag
    15421 taataaattc ttatcgttgt aaggatggaa agtggatatt cttaagtatt ttagagcatg
    15481 aaagatattt tgaggcactt tgtagattat ttggaagaga agacttaata aaagaagaga
    15541 aatttgcatc ttcagtagaa agtaagaaaa atgctacaga acttattaat attttggatg
    15601 gtgaatttgc aaagtttact caaaatgaga tggttgaaaa acttactgct gctgatatag
    15661 ctcatgaaaa aattcaacat gttaatgatg tagtgactga tgaacaggct attgctaata
    15721 actatatata tgagcataca agtaaaaatg gaaataagac aatgcttgca tccactccag
    15781 ttaaatttgg aaatattgaa gttaacatga cttgtgatgc tccacttata ggagaacatt
    15841 cagatgagat attgagagaa cttggataca gtgattctga tatagaagct ctaattgaat
    15901 ctggaattgt aacaatgaat aaagaagcat gtgaaatatg ttaataaaca tgtgaagtat
    15961 gttaataaat aggagggaat tttatggatt ttagattaac agaagcacaa ttaatgcttc
    16021 aaagagtagc aaaagaattt gcagagaatg aaatagcacc aatagctgct gaaacagata
    16081 aaacaggtat ttttccaagg gagcttttta gcaaaatggc taaaatagga tttaatggca
    16141 ttggaacacc tgtagagtat agtggttctg gagggacaga tatagaaaag gtaattgttg
    16201 ttactgagat tgccaaaaaa tgtgcagcat ctgcagcaat attatcaata cacacaatat
    16261 atgctcaagc tattttgaaa tttggtactg aggaacaaaa gaaaaaatac cttccaatga
    16321 tggctgaagg tgggtgtgta ggtgctttcg ccttaactga acctaatgct ggttcagatg
    16381 ctgcaagagc tgcaacaact gcaattattg atgaagaaac ggatgaatat gttttaaatg
    16441 gaactaagtg ttttatttct ggtggaggtc aagcagaatc attaataata tttgcactga
    16501 cagacccatc aaagggaata aagggaatgt ctgctatcat tgtcgacaaa ggtactccag
    16561 gtttttcaat aggaaaaatt gaagaaaaaa tgggtattca tggttcagaa actgcagagt
    16621 taatatttga taactgtaga gttccaaagt ctaacttatt aggaaaagaa ggcaaaggat
    16681 ttaatatagc tatgacttgt ttagatggag caagaattgg aattggagct caagctgtag
    16741 gaattgctga aggggcttta gaagaaagta tcaaatattc taaagaaaga gtccagtttg
    16801 gcaagccaat ttcagcgttg caaggtattc aatggtatat agcagatatg gcgacaatgg
    16861 tggaatcagc aaaattatta gtatattatg ctgcagattt gaaagctaga ggagaaaaac
    16921 acactaaaga agctgctatg gcaaaataca atgcgtcaag tactgcaaga gaggttacaa
    16981 atctagccct tcaaatccat ggtggatatg gatatatgaa ggattatcca ttagagagaa
    17041 tgtacagaga tgccaaaata acagaaattt acgaaggtac ttcagaaata cacaaagttg
    17101 ttatttcaag agcagtacta ggataggagg aaaagaatga atatagttgt ttgcttaaag
    17161 caagttccag atactaatga agttaaaata aataaagaga caggtactct aataagagat
    17221 ggtgtaccaa gtataatcaa tccagatgat agaaatgctt tagaagaagc tctaaaaatg
    17281 aaagatgaac taggagcagt gattaaggtt ataagcatgg gacctcctca agctaaaagt
    17341 gctttaaaag aagctttggc aatgggagct gatgaagctt atttaatctc agatagagct
    17401 tttggtggtt cagatacttg ggctacatct acaattattg ctgctgctat agaaaaggtt
    17461 ggaaagtatg atgtaatatt ctgcggaaga caagctatag atggagatac agctcaggtt
    17521 ggaccagagg ttgcagaatt tttagggata ccacaagtta cttatgcaaa agaagtaaag
    17581 gttcaagatg acaaattact agtaactcgt tatacagaga caggggatta tttaatagaa
    17641 gcaaagatgc cagttttatt aactgctata aaagagttaa acaatccaag atatccaagt
    17701 gtaaaaggaa tcttagaagc ttataataat ggagatgcga agattactgt attaacactt
    17761 gcagatttag atgttgatac aactcaaatt ggattaaagg gttcacctac aaatgtttat
    17821 aagtcttttg caccagttaa agacaagcat aatgaaataa tagaaggtat aaataaaaaa
    17881 gaaaaagctg agaaactaat agaaatacta ttcgatttga aactagtata ggagtgttaa
    17941 gatgatgaga gcaaaagtta atcaaggtat aaatttaaat gattataatg gtgtttgggt
    18001 aataggggag caaagggaag gcaaaattaa tcctgttacg atagaactta ttggtgaagg
    18061 aagaaagctt gctgaccaat taggaaaaga gcttgaagtt gtgatagctg gatatgaagt
    18121 agagaaagaa gtgaaggaat tacttcacta tagtgttgat aaagtatatt acatcaatga
    18181 ccctcttctt aaagatttca ctactgatgg atatgcaatt tcaattgcaa atttaatcga
    18241 aagaaaaaag ccagaggttg tattggttgg tgctacttct ataggaagag atatagcgcc
    18301 tagaattgca ggtaaggttg gaacaggtct aactgctgac tgtacaaaac ttgaaataga
    18361 ttcaacagat aataagttat tacaaacaag accagcattt ggtggaaatt taatggccac
    18421 tattgtttgt ccaaaaaata gaccacagat gtcaacagtt cgtccaggag ttatggcaaa
    18481 agctgtaaga aacgaatcag agactggaat tttagaagtt gttactccag aacttactga
    18541 aaaaatgatt agaactagat tagtagaggt acttccacaa gaaaagaaat ctgtaaatct
    18601 aactgatgct agaataatag tatcaggagg aagaggatta aaaagagcag aaggatttga
    18661 gcttataaaa gaattggctg ataaattggg ggcagagatt ggtgcttcaa gagcagcagt
    18721 agattcaggg tggatagaac attctcatca agtgggacaa acaggaacta ctgttagacc
    18781 agaactatac attgcatgtg gaatatctgg agcaatacaa cacttagcag gtatgagtga
    18841 ttctaaatat atagttgcta taaataaaga tgcaaaagcc cctattttta gtatatgtga
    18901 ctatggaata gtaggagatt tatatgaaat tatacctgaa atgatagaat cattaaatag
    18961 gtaactttta gaaaacattg aatatgtaga atgataagtc ttataatatt gtattgtaag
    19021 acttattatt tttgctgaag gttatagtgg ggagatgaaa caatgaaaga gcagtggtat
    19081 aaggatatat ttgcaagaat attatctatg actgatgatg gttttattgt tgtaaataca
    19141 agtggagtaa taatagatat taatgataaa tattgtgact ttttaggaaa agaaagaaaa
    19201 gatataatag gacaaaatat acaaagtatt ataccaaata caaaaatgct agatgttatg
    19261 aaagataagt actgtgaaga aggagctata catcattatt caggtggaaa tacaaaagaa
    19321 aaaagtgtaa tagtaagtag gtcttatgta gaaaatgaca atggagaagt agttgcagga
    19381 gtagctcaag ttaaatttag attgcaatca tttgatgtag ctaaaaaatt aatgtcagaa
    19441 tatatggagc tccaatatta taaagagcag tttaaggata actgcggttt tgacaagctc
    19501 ataggtgaaa atagagactt catagaactt aagaaaacag gtgtgaaagc ttctaaaaca
    19561 aactttccag tacttttaac gggtgaaaca ggcacaggta aagaagtctt tgctagagca
    19621 attcacaata atagcagtag gtcagataaa cctatggtta gtataaactg cgctgcaata
    19681 ccagaagaat tattagagtc agagctattt ggttatgacg aaggagcttt tacaggagca
    19741 aaaaagggtg gtaaaaaagg taagttttta gttgcaaata atggtacaat ctttttagac
    19801 gagataggtg acatgccttt gaccatgcaa gcaaaacttt taagggttct tcaggaaagt
    19861 gagatagaac cagtgggagg tcttaaaact ataaagatag atgtaagagt aatctctgcc
    19921 acaagaaaga atttaagtaa aatggttgaa gagggattat ttagagaaga cctatactat
    19981 agattaaatg taataaacat tcatatgatg gaattgaagg acaggcaaga tgatatttta
    20041 ttgctagcaa attatatatt aaacaaatta aatgttgaat acaaggaatt gaaggtttta
    20101 agtgataagg ttaaaaattg ttttattaac tatacttggc cgggaaatat aagagaacta
    20161 caaaatgtaa taaaaagtgc ctatgctgta agtgatgata tggtcattat gatgtgtgac
    20221 cttccatcta agatggataa tatcagcaga gtagctcaat gtaatgtgga tagcaattgt
    20281 tcaattcatg aaatggttga gaattatgaa aaaagcttaa ttattgatgt gttaaggaag
    20341 tataattgga aatgttctaa ggcagcagag gttatgggaa tacacaagag tcttttatat
    20401 aagaaaataa aaaagtatga aattgaatta aataattaag ataacttgaa ttaaggcaat
    20461 gctaaaaaaa gcgcccttat tcaaagcgaa ctgtataaac tgttttatgc agttaaaacg
    20521 tgtaaaagta gatgtgaagt actaatgtca atcattagta ctttttattg tatttataat
    20581 tactatctat atttcagtat cttttgatat ctaagattat tatgtttaat ttaggatata
    20641 acaaaaccct cttagttatt gacagttttt tatattttaa ctgtctacta agaggggata
    20701 gtttagttta aatttagagt aaattttatc tagcatttat aaaaaatatt ttgaatagct
    20761 tttttgttat ttagtcataa ttttctactt ctttatcaag ttctttgcca caagaatcac
    20821 acttatatat agcattgtat ccataatcat attcatcaac tattttcttt tgtctaaata
    20881 caccaccaca ctcacatagg aaaccaattt ttataagtgt aacgataaat tgtgatttta
    20941 taaattttat atcattacat ttatcgttag tgtaaaatgt ataaatataa gagttatcgc
    21001 actcatctct taattcaaat ctttcaatat atgtgttatt ttttaattcc tcaaattctt
    21061 ctttagttaa atatgatttt tgttgcaatt catgtaaacg cttaatttgg tccattattt
    21121 atatcctccc aataatttta gtattttaag cttagataag gaatctaagt tttattaaat
    21181 ttatcccaag gaattattta aatcatattt agttttatta attacaacat atataaatta
    21241 cacgaaaata aaactaattg taaaaataat atcctgtgaa aacctaaact actaacccct
    21301 tactaacccg aaaactaaaa aaagaaaata tatccaaata taagtgatat ataaataaag
//
LOCUS       DAELSY010000063        21182 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.063, whole genome shotgun sequence.
ACCESSION   DAELSY010000063 DAELSY010000000
VERSION     DAELSY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21182
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.063"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     repeat_region   653..1009
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=653..681
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            1248..1436
                     /locus_tag="KPG09_002041"
     CDS             1248..1436
                     /locus_tag="KPG09_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966651.1"
                     /translation="MKIITDSSRADYFKQRRQNKKTFSVLLDREKVEKIEEHLKKQNK
                     TKTIWLEEKINEELEKEE"
     gene            1555..2421
                     /locus_tag="KPG09_002042"
     CDS             1555..2421
                     /locus_tag="KPG09_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HBG0966652.1"
                     /translation="MKNLIVKEFNGSQIYTFMWKEKSCWIANQIVGLFDYADVSKTIQ
                     DCIKAEDFEIEQEYDVLKGNEFNDFVTTLNVVANNIISNKARSITIFYEDGLYGFLQY
                     TDKPIGVQFRKWLRREVLPSIRQTGAYITNNANPEKLREKASEIEKLQLAYNSTSMLK
                     ELLDGAGFDNKSKLLTAKTLYKKAGIDLPIEINEEEHYFDTKQIASKLKIYSKSNKPA
                     QLAVCEIIKKIDLEENEVKGVWETNGSWTGTVNKYTKSVIDKVRNWIEENNRPTKIAG
                     EKKNYHVVYKIE"
     repeat_region   2874..3163
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2874..2902
                     /rpt_unit_seq="gttttatattaactatatggaatgtaaat"
     gene            3286..3813
                     /locus_tag="KPG09_002043"
     CDS             3286..3813
                     /locus_tag="KPG09_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBG0966653.1"
                     /translation="MREGKRRRGCLFWFILIILFSGVVGAIAGNSTNNESTEKQKEDL
                     TKYIGEEGSIGDLKLTVNSISKASEISVASGYLAYTPDSGKYGIINLTIKNQTKESQS
                     FMLNSFTLIGPDDSKYVPSLLIDVGNKYITMDTVNPNLDVTGNIAFEIPKDLLVSDCK
                     LKYSGSEQEFILKEK"
     gene            3951..4718
                     /locus_tag="KPG09_002044"
     CDS             3951..4718
                     /locus_tag="KPG09_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HBG0966654.1"
                     /translation="MGLFGKKKADICCICNTEIGVLNIEDGWICNSCFKEYCDALSMT
                     KAPKILRKLDIEKTISSTKKNNELQKIFNTTNNIENYIEFDEDNKKWLVSKKSINDKK
                     TPIIHSYEDIVKFELLENGETVTKGGIGRALAGGILFGEAGAIVGGITGKKTTRKVVD
                     TFKIKITINNIDNPIEYIELINKKTKTNSSAYEKAYKDAHKILSTLSAITQSIKETDN
                     INTKSVADEILKYKNLLDMEAITQDEFNTKKKELLNL"
     gene            4784..7156
                     /locus_tag="KPG09_002045"
     CDS             4784..7156
                     /locus_tag="KPG09_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBG0966655.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTTSINTTINSLERLQQRLHNPLN
                     TGGIQASQQSLNNIENILTRIEQKIGRNTNEQENFNNKIKQGSEAGSLLVSKLKSLAG
                     IYIGIRGIESITKAADTIASTKARLNLMNDGLQTTEQLNKMIYLSAQSARASYADTAA
                     QVAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMPVTFSQALTMMKNDAYMIFGQTLGKISGALQSVRFSEIVVSIRNVMIAISSNIYDT
                     LNIIKNILNSEFFSNLVQGFTFCAVVITKSLGSIVNTALNIVNIFAQNWSIIKPIVFG
                     VASVFILFRGVLLATKIATIGNVIANVAHAASSSLSALMTNIQAAALMRSNGATLSAT
                     IATWGLNAALLACPITWVVLGILAFVVVVFVAVAAVNKFAGTSLTALGVIVGAVFAAV
                     AAIQNVMIWLLNGCIAVNEGITNGWNQCVYLMKQAIAKGVIFIIEKMASLNNSVNNAG
                     NALGKAFIDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYIKQQIDSL
                     NRWVGDAPEKVKLERMGYKDIGAAFQKGNALGTKWQNAITDKFKDTFDINKMLEDAKK
                     KLGLDDLWNKQNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQ
                     ETINRFTGVNIKIDMNNTNNISKDTDVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            7173..7862
                     /locus_tag="KPG09_002046"
     CDS             7173..7862
                     /locus_tag="KPG09_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG0966656.1"
                     /translation="MAYDFYLDGVQLPIAPPKLEVKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDADNGSDTKVAIKLKQYRDYSTKKLVLAPPKNETGRPNVKIEPK
                     RVDSVNATNTKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKSMMDKANKGKNLSKY
                     TIYKGQVLKLG"
     gene            7855..9747
                     /locus_tag="KPG09_002047"
     CDS             7855..9747
                     /locus_tag="KPG09_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0966657.1"
                     /translation="MVDELVLANDRDVRLVIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKILAKDFNLKYNVIEDTKYKISRVEENKTLFDMILTALDDTLRE
                     KKEMYTLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSAHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     ALGDIRVRAGCLVPVFLNLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSIMKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLNKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSNTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            9761..10021
                     /locus_tag="KPG09_002048"
     CDS             9761..10021
                     /locus_tag="KPG09_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBG0966658.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESISPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKMGDKLVLIRMQGGQQYLILDRM"
     gene            10026..10445
                     /locus_tag="KPG09_002049"
     CDS             10026..10445
                     /locus_tag="KPG09_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG0966659.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLHIEKSKIDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISYVIPELERRITEALIQDDR
                     IENIDNFEFQNIKGKVQCRFSVHTKYGNIKAEKVVSV"
     gene            10446..11495
                     /locus_tag="KPG09_002050"
     CDS             10446..11495
                     /locus_tag="KPG09_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG0966660.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLEATHTIAKGVFNIDIPLDSRFNLGE
                     YNYVAIERISEKTYKMKCETTGPVFELGKLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYRDEVNKIQDVGGVKVYPTWDGGGTVKLVIINSNFKV
                     PSEELVNLVQEEIDPIGHQGEGLGLAPIGHRVTVEGVTSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINDVKSNL
                     TIDSNSIVVRGEVVG"
     gene            11488..12105
                     /locus_tag="KPG09_002051"
     CDS             11488..12105
                     /locus_tag="KPG09_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBG0966661.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            12117..13142
                     /locus_tag="KPG09_002052"
     CDS             12117..13142
                     /locus_tag="KPG09_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG0966662.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTELKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNINNIELKIDPTIIFATLKDIQDLDAK
                     ISNVNTKIDTTKTELTSNIETAKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTIT
                     IDDWIDNQEGGFKATVTHGLLTQRITVSIIDATTKDNVVPDFTIIDDNSIEVRSGVKV
                     ELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIHEEIGKVAEQLTGINSNIISTV
                     NNDILPI"
     gene            13159..14685
                     /locus_tag="KPG09_002053"
     CDS             13159..14685
                     /locus_tag="KPG09_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966663.1"
                     /translation="MALSMSYFNLPDKRKYTKNLAFNPFAGGRQNFEWTGGDHGLNGE
                     FKETCLSCTYNGSTLNWGSGNVWVLGEYGQYTFTYNCESMHVDTQQKFPYTSNRIITI
                     KGRPVISGSDTSLGNKRKGFSVDFTVSDDTPNVNLIVRAYLDDKLIQNITPVVQNSTL
                     TATVTDSQLNSLSVDGNHKLKIQLNDGYDNFDRIFTFKKIEKGIDISTSLVTDSQAKF
                     TVTKIYSELTKIECYLDETLKETFTTDLYSEKTINYELIDNAIHTLKIVVTDAENVVE
                     EKVISISKNIMPLQPDATLQDISTKLTEIGQGVRNGKTSIINTLALKNIDASLNNTLV
                     ELSEKIKGGFDSGDASLQDLMNQLTQANNTISQLNTKYKVASGTVTSFADSTKIAYPY
                     LTDNVTKPGSWIKVSNLGFKPNIFFADFDYYDAEYKNNYKLFLFACNGVATQRGVDFS
                     SVTSFIRKSGDEYFHANGWLYSNSEGDVYFNNTGVQIPAYNFDSTQKHTYKWYAIKFI
                     "
     gene            14700..14993
                     /locus_tag="KPG09_002054"
     CDS             14700..14993
                     /locus_tag="KPG09_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966664.1"
                     /translation="MNVPNRIIYDQTGRTIFETGESCGDVLPHYTITELHYIDIEYGS
                     IDYTRNRVIGINIETKEPILEEIPVYITDEEKRIQELENQLLIAENEKVGGLL"
     gene            14993..15175
                     /locus_tag="KPG09_002055"
     CDS             14993..15175
                     /locus_tag="KPG09_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966665.1"
                     /translation="MNINNVVVRILAERILSKGLNPLKNREFQLDDVTNTEYRKAVED
                     YIIKNSGVVEGAEPTI"
     gene            15209..15775
                     /locus_tag="KPG09_002056"
     CDS             15209..15775
                     /locus_tag="KPG09_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966666.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTESKFTSENVEGALKECIDR
                     ADEAFQEADSGKTLLSTAIGSPTTSEQTFQDYANYITGFKSNISNLETQLKSKYSIRY
                     GPIDGYDGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITINS
                     NKTGFSVHSYDTSYESYPFTGYYIACFA"
     gene            15806..16096
                     /locus_tag="KPG09_002057"
     CDS             15806..16096
                     /locus_tag="KPG09_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966667.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNNSNRKIQDETETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKIEASTKQAHLRIDEIVNRIGGK"
     gene            16100..16357
                     /locus_tag="KPG09_002058"
     CDS             16100..16357
                     /locus_tag="KPG09_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG0966668.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGINQTYKQLKDGEK"
     gene            16618..17481
                     /locus_tag="KPG09_002059"
     CDS             16618..17481
                     /locus_tag="KPG09_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HBG0966669.1"
                     /translation="MVEVKEEKTFDEQIDILKSRGLIINDKEDAKFVLSNVNYYRFTA
                     YLLSFKNDDGSYKEGTTFEEVYDIYRFNKEFRILLTDLLGSIEIAFRTYIAYTLAIKH
                     GACGYLERESFKDEKFYINFLTALEREKSNNSDKLFIIHHKEKYEGKLPIWVATEIMT
                     FGMLSKLYSNMLPEDTRYIKNNLCRVNTLLVKSWLQSLTQVRNQCAHYGRIYNNNFRI
                     ITIKNEYKKYNLDNKKIFSYILAMKHLTMDKLIWNSFFIKLQKLINDYNNSIDLKLIG
                     FPNNWIEILAK"
     gene            17560..18372
                     /locus_tag="KPG09_002060"
     CDS             17560..18372
                     /locus_tag="KPG09_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0966670.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSNPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFINR"
     gene            18466..20865
                     /locus_tag="KPG09_002061"
     CDS             18466..20865
                     /locus_tag="KPG09_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG0966671.1"
                     /translation="MIFKKYIKSLLIVVSVTLIMNKSILFVNALDLESNNNIKSYKRK
                     NVKEIDISNKFVDESFRRDIVEKVLDNRDDPLTNEVASKDNYKIYESDLNKIESCLEL
                     DLHKNPDVSNPNKIDNLDGIENFRNLEKLDCSSNNLESVDLSKNIKLKYLDISGNKIL
                     NIDLKNNNLINELHCDANKLSNLDLKNNKNLVSLFFTSNELTEIDLSNNVNLENLQFL
                     NNNLKSIDLSHNKKLKSLNCTNNKLKSLDLSNNVFLKNLSCSGNNLSKLNIKGLTSLE
                     YLGCAKNKLQDLDVSTNIKLDSLLCGKNSLTKIDISKNKELNSLECSGQYIEKSIIKN
                     GENIVVDIGIENSKLKELYPQNYNISTGFLTWENLSDFPNSISYSLEYEYGPVNDTET
                     MDVNISIDKLVKCRIRYIDYDGSIINEKEISDNSSINEIQINPQRENYEFSGWRNNKD
                     EKIYTSEQVSKLVFDDSTDFIATYKRKSTGSSGSSGGTSSKEDFNIAPSKEKITGADR
                     NETSVKISQKGWNKADNIVLINDSSISDALSATPFAKSKDAPILLTKNNNLNKLTEKE
                     INRLEAKNVYIVGGLKSVDEKVVSDLKKKGLNVIRISGNDRYETSIKLAKELDKNSNL
                     SKVVVVNGEKGLADAVSMGAISAKEEMPILLTNQNDDMKDIKDLIANKNISKSYVIGG
                     ESLFNNKEVNNTLPSVTKIAGSDRTETNSKVISHFYSKDTLNDLYVAKNGMNKQDDLV
                     DALSVGVLAGKTESPVVLVGNGLDDSQKELIKNKKFKNITQIGGNGNEKAFNEVENLV
                     K"
     gene            complement(20906..21091)
                     /locus_tag="KPG09_002062"
     CDS             complement(20906..21091)
                     /locus_tag="KPG09_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBG0966672.1"
                     /translation="MILLEQRKLRIGFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
BASE COUNT         8635 a         2283 c         3812 g         6452 t
ORIGIN      
        1 attgtttata taaaataaaa aataaaaatt tttattaaaa aaatgaaaac ttgattataa
       61 agcaattaat ttataaaata tatataaata agtaggtatt tatttacttg aatttcattg
      121 tttatataaa aaaaatggaa aaatatgtaa taattatagg ttataataat tgtagcaagg
      181 ataataatcg aaagtgcgaa gggtgattat tttcatatta aacgccaaat tccaaataag
      241 gaaggaggtg aaattatatg ataggttttt tattaagcat actagctggt gttatatcag
      301 cttatattta tgacaaaata aaaaatcacc cagacgccaa taagggtgat ttaaaaaaat
      361 aatattttca cttaacaact gaaaataatc actctttgta ggaataaatt atttccttgc
      421 ttttattata ccacaaattg gtacagatat tcaaaaataa tatttttatg atataataaa
      481 aatgtagaga ttttgcagtg agcgatattt gtgataaatt gaagtttaac agttgcaata
      541 taaggcgttg agggtgtgtg ataaatgtta tcaattgcac tactcatggt tcactgcaaa
      601 tttaagagag ttgtatatgt gtaggtattg aaaatactca acttattttg gggttttaga
      661 ttaactatat ggaatgtaaa tgttgtaaga agtatcattc tattttttaa tctttctgtt
      721 ttagattaac tatatggaat gtaaatttca gtgagaataa gctttattgt cgatgtaaca
      781 ctcgttttag attaactata tggaatgtaa atagtacata taatgagtct ttaacatcag
      841 ttatgaaagg ttttagatta actatatgga atgtaaatga ttgtacttta gcgtctgcac
      901 tagctttgtc tatcgtttta gattaactat atggaatgta aattatttta cagatgaaca
      961 attacagtta cttcttgaat gttttatatt aactatgtgg attcaaaatt aaatagaaaa
     1021 aagaaagcac ttacttaaat agtaggtgct tttgttttgc tcaaattact taattataaa
     1081 gttgctaagt acaacttttt agaattatac aatataaaaa tgcactaaag tgattgattt
     1141 tgctcaagtt ggtcggttga gtaaaataat tagaaaaaaa tgttaaaaaa ctcttgaaaa
     1201 gtgtcgcgat acaatgtata attatattat cgcgatacag aaaagaggtg aaaattatta
     1261 ctgatagcag tagagcagat tacttcaagc agagacgaca gaataaaaaa acttttagtg
     1321 ttctactaga tagagagaaa gtagagaaaa ttgaagaaca tttaaaaaag cagaacaaga
     1381 ctaaaactat ttggcttgaa gaaaagatta atgaagagct agaaaaagag gaataaaaaa
     1441 taagagacgt tctccccgac caaagattga acatccctta ttgacgtata ttatatacac
     1501 taactatagt atacgtcatt ccttgaaaaa attcaattaa ggagtgtaat agttatgaaa
     1561 aatttaatag taaaagagtt caatggaagt caaatttata cttttatgtg gaaagaaaaa
     1621 tcttgttgga tagctaatca aatagttgga ttattcgatt atgctgatgt atctaaaaca
     1681 atacaggatt gcataaaagc agaagacttt gagattgaac aagagtatga tgtattgaaa
     1741 ggaaatgaat ttaatgattt tgtaactact ttaaatgtag tcgcaaataa tataattagt
     1801 aataaagcta gaagtataac tattttttat gaagatggtt tatatggatt tttacaatac
     1861 acagacaaac ctattggtgt acagtttagg aaatggctta gacgagaagt tttaccaagc
     1921 ataagacaaa ctggtgcata cataactaac aatgctaatc ccgaaaaact aagagaaaaa
     1981 gcaagtgaga ttgagaaatt acagttagct tacaatagta catctatgtt aaaagaacta
     2041 ttagatggtg caggctttga caataaatcc aaactattaa cagctaaaac attatataag
     2101 aaagcaggaa ttgatttacc aatagagata aacgaagaag aacattattt tgatacaaag
     2161 caaatagcat ctaaactgaa aatatattct aagagtaata aaccagcaca gttggctgtt
     2221 tgtgaaatta ttaaaaagat tgatttagaa gaaaacgaag tcaaaggcgt ttgggaaact
     2281 aatggttctt ggactggtac tgtaaataaa tatacaaaga gtgtaataga taaggttaga
     2341 aattggatag aggaaaataa tagacctact aagattgcag gtgaaaagaa gaattatcat
     2401 gtggtttata agattgagta aatttataag attgtattaa ataatttagt ttagttttgg
     2461 ggggattaat acaatgcatg agaatttact tgatagtata gatattgaaa agagaaaaga
     2521 agaatttaga attaaacttt tgaaaataag agaaacagat atagatatat ataataagat
     2581 agaaagtata gtatataaac tttcttagaa aaaattagag aaaaataatt aaataaatag
     2641 ataaagcact tggatatttc actgtttcaa gtgctttata tggtaaaaaa tggtataata
     2701 aaatatagga attttgcagt gttcgatttt tctactcaag tatagtttaa caattggaat
     2761 acaaggcatt gagaaggttt gataagtgtt atcaattgca ctattacccg ctcactgcaa
     2821 atttgagaaa gttatatgtg tgtaagtctt ggaaatacac aatttatttt ggggttttat
     2881 attaactata tggaatgtaa atctttgtaa tggtagtgta tttaagattg aaacatcaag
     2941 ttttatatta actatatgga atgtaaatca acattagtag ttgtctttat acacatagca
     3001 tcacgtttta tattaactat atggaatgta aatacagctc ccaagacata caacgaatct
     3061 gtaacatcag tgttttatat taactatgtg gtatgtaaaa gacttattta cagctttatt
     3121 tgctaaatca gaaggtttta tattaactat gtggacttaa aattaaaaat aattaaaaaa
     3181 cacttactaa tgtaggtgtt tttttattga aaggaagtga ttataatgta aaaattttga
     3241 agatatagta taatattctt ataaaatata atttaggggg atattatgag agaaggaaag
     3301 agaagaagag gatgtctatt ttggtttatt cttattattt tattttctgg agttgttgga
     3361 gcaatagcag ggaacagtac taataatgaa agcactgaaa aacagaaaga ggatttaact
     3421 aaatatattg gtgaagaagg tagtatagga gatttaaaat taactgttaa tagcatttca
     3481 aaagctagtg aaatatcagt agcatctggt tatttagcat acactccaga tagtggcaaa
     3541 tatggtatta taaatttaac aataaaaaat caaactaagg aaagccaatc ttttatgcta
     3601 aattcattca cattaatagg accagatgat tcaaaatatg ttccatcttt attgattgat
     3661 gtaggaaata aatatataac tatggatact gtaaatccta atttagatgt aacaggtaat
     3721 attgcatttg aaattcctaa ggatttacta gtttctgatt gtaaactaaa atatagtggt
     3781 tcagagcaag aatttatatt aaaagaaaaa taatatatag atttaaaaat atgaagcatt
     3841 tactttaaat aagtaagtgc ttttttaatt aaaaggaggt gataataatg taaaaatttt
     3901 acttatatag tataattaca ttataaaatt attgtactgg ggggaatctt atggggttat
     3961 ttggaaagaa aaaagcagat atatgttgta tttgtaatac tgagatagga gtactgaata
     4021 ttgaagatgg ttggatatgc aattcttgtt ttaaagaata ttgtgatgca ctttctatga
     4081 ctaaagcacc taaaatttta agaaaattag atattgaaaa gactatatca tcaactaaaa
     4141 aaaataatga acttcaaaaa atatttaata caactaataa tatagaaaac tatatagaat
     4201 ttgatgaaga taataaaaaa tggcttgtgt ctaaaaaaag tataaacgat aaaaaaactc
     4261 ctatcattca ttcttatgaa gatatcgtga aatttgaact tcttgaaaat ggtgaaactg
     4321 taactaaagg aggaatagga agagctttag cgggaggaat tttatttgga gaagcaggag
     4381 ctatagttgg agggataaca ggtaaaaaaa caacaagaaa agttgttgat acatttaaaa
     4441 ttaaaataac aattaataat attgataatc ctattgagta tatcgaatta attaataaaa
     4501 aaacaaaaac taattctagt gcttatgaga aagcttacaa agatgcccat aaaattttat
     4561 caacattatc tgctattaca caaagcatta aagagacaga caatataaat actaaatctg
     4621 tagcagatga gatattaaaa tataagaatc ttttggatat ggaagctatt acacaagatg
     4681 aatttaatac taagaaaaaa gaattattga atttgtaata taataaacac ttactaatgt
     4741 aggtgttttt ttatatggaa atttatgaaa ggagagtgag aaagtggcaa cgatacaaac
     4801 ttcaataaag attttcgacg gaatgacacc tgcttttagg aatatgacta catctattaa
     4861 tacgacaatt aatagccttg aaaggttaca acaaagattg cataatccgc ttaacacagg
     4921 tgggatacaa gcatctcaac aaagtttaaa caatattgaa aacatattaa ccaggataga
     4981 acagaaaatt ggaagaaata caaatgaaca agaaaacttt aataataaga taaagcaagg
     5041 aagtgaagca ggttctctat tagtatctaa actcaaaagt ttggctggca tatatattgg
     5101 aataagaggt atagaaagta ttacaaaagc agcagataca attgcaagta caaaagcacg
     5161 tttaaattta atgaatgatg gattacagac aacagaacaa cttaataaga tgatttattt
     5221 atctgctcaa agtgcaagag ctagttatgc agatacggca gcacaagtcg ctaaacttgg
     5281 aatattagca ggagatgctt ttggaagttc ggcagaggtg ataaagttta cagaacttat
     5341 gaataaagct tttgtaattg gaggaacatc agcaaatgaa gctagtgcgg ctatgtatca
     5401 gcttactcaa gctatgggtg cagggaaact tcaaggtgat gaatttcgct ctataatgga
     5461 aaatgcacct ttattagcca ctaaaatagc tgatgcaatg ggaaaaacta aagaccaatt
     5521 aaaggaattg tcaagtagtg gagcaataac agcagatgtt ataagaaatg cactatttaa
     5581 agcttctgat gagatagaaa agaaattcgc aagtatgcca gtcactttta gtcaagcact
     5641 tactatgatg aaaaatgacg cctacatgat ttttgggcaa accctcggaa agataagtgg
     5701 agcattgcaa agcgttagat ttagtgaaat tgttgtatct ataagaaatg ttatgattgc
     5761 aatatcttca aacatttacg atacattaaa tattataaaa aatattttaa atagtgaatt
     5821 cttttcaaac ttagtgcaag gatttacatt ttgtgcggta gtaataacta aaagcttagg
     5881 ttctattgta aatactgcat taaatatagt taatatattt gcacaaaatt ggagtataat
     5941 aaaacctatt gtatttggtg tagcttctgt ttttatattg tttagagggg ttttgctagc
     6001 gactaaaata gctacaatag gtaacgtaat tgcgaatgtt gctcacgctg cttcctcttc
     6061 actatcagca cttatgacta atatacaagc agcagcactg atgcgttcga atggagctac
     6121 tttatcagca acaatagcca cttggggttt aaatgcagct ttattagctt gtccaatcac
     6181 atgggtagta ttaggtattc tagcatttgt agtagtagtt tttgttgcag tagcagcagt
     6241 aaacaaattc gcaggtacaa gtttaactgc cttaggagta attgtgggtg ctgtatttgc
     6301 agcagtggca gcaatacaaa atgttatgat ttggttatta aatggctgta tagctgtaaa
     6361 tgaaggcatt acaaatggtt ggaatcaatg tgtatatcta atgaaacaag ctattgcaaa
     6421 aggtgtaatc tttataatcg agaaaatggc atctctaaat aattctgtaa ataatgctgg
     6481 aaatgcactt gggaaagctt tcatagatgg ggcaaatatc gcaatacgag gtgtaaacaa
     6541 attaattgac ctaataaata aaataccagg gataaatatt ggtaaagtag gagaggcaac
     6601 gtttacgcca gttaaggcag ataatagtta catcaaacaa cagattgata gcttaaacag
     6661 atgggtagga gacgcaccag agaaagtaaa attggagcga atgggataca aagatattgg
     6721 agcagcattt caaaaaggaa atgcacttgg aactaaatgg caaaatgcta taactgataa
     6781 atttaaagat acttttgaca ttaataagat gctagaagat gcaaagaaaa aattaggatt
     6841 agacgatttg tggaataaac aaaatccttt aaacaacctt ggtggatttg gtggagattt
     6901 aggaaaaaat gtaaaggaca cggcaggcaa tactgcaaag atggcaaaga ctatggataa
     6961 aagtcaagag gacttaaaat acttaagaga tatagcagag caagaaacaa tcaatcgatt
     7021 cacaggagta aacattaaaa tagatatgaa caatacaaac aacataagta aagatacaga
     7081 tgttgatgga atagttaatg tcttaactga aaaactgaac gatgctatgg ttgtatcagc
     7141 ggaaggaata gtttaggaag gagagtgaga aaatggctta tgatttttac ctagatggag
     7201 tacaactacc aatcgcacca cccaagcttg aagtcaaagt gacaaacaag aacaagacag
     7261 ttgatttgat aaatactgga gaagtaaaca tattaaaaaa agaaggatta tctgaaataa
     7321 gttttgaagc agaatttaca cataataaac tacctttttg tagaggtcaa tttagagatg
     7381 ttcaattctt tttaagtaag ctagaattac taaaaactga ttgtaagcca tttcaattta
     7441 ttgtatctcg tgagttaggt aacaaggtct tatttaacac taatataaaa gtatctcttg
     7501 aagagtatgc tatttcagaa gatgcagata atggctcaga tacaaaagtt gcaataaaat
     7561 taaagcaata tagagattac tcaactaaaa agttagttct tgcccctcct aaaaatgaga
     7621 ctggtagacc taatgtaaag atagagccaa aacgagttga ttcagtcaat gccacaaaca
     7681 ctaaaacata tacagtaaaa gcaggggata gcctttggtc aatttgtcag aaacaacttg
     7741 gtaatggttc attatataag aaagtatacg aactaaataa atctatgatg gataaggcaa
     7801 ataagggcaa aaacttaagt aaatacacta tttataaagg gcaggtgtta aaacttggtt
     7861 gatgaattag tgttagcaaa tgatagagat gtaagattag taatagctca ttgggaagat
     7921 ttctacgaac ctgctgtcat tgatggtatc acatgggaga tagaaagacg aggtacacct
     7981 tctaagttag agtttacaat agtcatggat gatatactag agttttgcga aggaaattct
     8041 gtaaggctgt attataaagg aataggcata ttttatggat atatatttca aaagaaaaga
     8101 gataaagaaa atcacattaa aattgttgct tacgaccagt tgagatattt taagaataaa
     8161 gatacttatg tatatagtaa taaaactgca agtgaacttg taaaaatctt ggctaaagat
     8221 tttaatttaa aatacaatgt catagaagat actaagtata aaatatctag agtcgaagaa
     8281 aataaaacac tctttgacat gatactaaca gcactagatg atactttaag agagaaaaaa
     8341 gaaatgtata ccttgtatga tgattttgga agaataacat taaagaatgt tgcatcaatg
     8401 aaactagata ctgtcatgaa caatgatgta attgaggatt ttgactataa ttcatcaata
     8461 gatagtgata cttacacaaa gattaaactt gtaagagaca acgaggagtc aggaaaaagg
     8521 gatgtatata ttgctcaaga ctcagctcac atgagaagtt ggggaatact tcaaatgttt
     8581 gatacagtag acaaaaacat gagtgaagca gagataaaac aaaagtgtga tatacttcta
     8641 aaactatata ataagaaaac taagtcatta agtttaaaaa atgcacttgg agatattaga
     8701 gtgagagcag gttgtttagt acctgttttt ttaaatctag gagatattga attgcaaaat
     8761 tatatgttag ttgagaaagt aaaacataca tttgaaaata attcacattt catggatttg
     8821 accttggttg atggagacga atttgcttca tattcttcaa gctcatatag tagtggaaat
     8881 actaataata aagatgaaaa gaaaaatggt cctgcacaaa gtattatgaa aaaaaataca
     8941 ggtaaaaaag ttcctgctat atttactgca tattatccag gaaacaatgc aatggaaggt
     9001 ggaaaaacag attgcaatgg aaagccactt gatgtaaaat caagaactgt tgctggtcca
     9061 atgaatcgag aaggagttaa gaaaacttgg tatactgatg attttctaaa taaacatcca
     9121 gtttttgaat atggagataa agtaaaaatt atacttcctg gtactgccta tgacaacaaa
     9181 gtatatacag ttaaagataa tggaggaaga atatatgttg aaacaaacgg aacatatcat
     9241 atagatatac tattagctaa tgctagtgaa tgtaaaaaat ttggtagaaa gaatggctat
     9301 ataattatag gtggagatga agaacaaaca tatcaagttg aaggtaataa ccaaagtaat
     9361 acaaataaca attctaaaga agataaatta attagtatag caaaaagtaa actgggttgt
     9421 aattatgtgt atggagcaga aggtcctaat aattttgatt gcagtgggtt tactcaatgg
     9481 tgttataaac aaataggtat aaaaattcct cgtactgctt ctgcacaaag taaagcagga
     9541 aaagcagtag atttaaaaga tagaagcaag tggaaagcag gagacttatt atgtagaatt
     9601 ggtggaggaa gtagtaatca tgttgtaatg tacattggaa acaatcaaat aattcattca
     9661 ccacaaacag gagatgtggt aaaaatagag tctgttaatt cttatagaaa aggaaaagca
     9721 tacacacatg tgagaagatt tatataagtg aggtggcaat atgagccaag atttattaca
     9781 gataataaaa aaagctgcaa tggatgcagt agaaacaagc aacccaatga gggttgtatt
     9841 tggaacaata gaaagtatta gtcctctaag agttaagata gaacaaaaac tatctattgg
     9901 tgaaattttt ctaatacaaa cagatacatt taaaagatat acagataaaa aaatgggaga
     9961 taaattagtc ttaattcgta tgcaaggagg gcaacaatac ttgattttag ataggatgtg
    10021 ataaagtgtt accaagtgat aatttagatt atgatattga agatgtatca ataattaatt
    10081 ttgatgtaag acaagagcca agtaagacat ttaaacttca tatagaaaag tctaagattg
    10141 atggtatttg tgatgatgta gaagcattaa aacaaaccat ttttttaatt ttaaatactg
    10201 aaaggtatga gcatcttatt tattctagaa attatggtgt tgaattaaat gatttaattg
    10261 gagaacctat ttcatatgta atacccgaac ttgaaagaag gataacagaa gcactaattc
    10321 aagatgatag gattgaaaat atagataatt ttgagtttca aaatataaag ggtaaagtac
    10381 aatgtagatt ttcagttcat acaaaatatg gaaatataaa agcagagaag gtggtgagtg
    10441 tataattgtt tgagttaatg acatttgaaa atataattaa aagaatgtta gatagtgtac
    10501 cagatacttt tgataaaagg gaaggttcta taatatataa tgctcttgct cctgttgcta
    10561 tagaacttac agaaacatac attgctatgg atgaattact agaccaaaca ttcgtagata
    10621 ctgctagtta ttattattta gagaagagat gtaaagaaag gggaataaca ccacttgaag
    10681 ccactcatac gattgctaaa ggagttttta acatagatat tccacttgat tctaggttta
    10741 atctaggaga atataactat gtagcaattg agagaatatc tgaaaaaaca tataaaatga
    10801 aatgtgaaac tactggacct gtatttgagt taggaaaact aatacctatt gaatatatag
    10861 atggtcttga aactgctgaa ctaactgaaa tcttgataaa tggagaggat gaagagtcag
    10921 aagatagttt aagacaaaga tattatgata gcctaaattc acagagtttt ggcgggaata
    10981 tacaaaacta tagggatgaa gttaacaaaa tacaagatgt tggaggagtt aaagtttatc
    11041 caacttggga tggaggtggg actgttaagt tagtaataat taactctaat tttaaagtgc
    11101 caagtgaaga attagtaaac ttagttcaag aagaaattga cccaattgga caccaaggag
    11161 aaggcttagg attagcaccg attggacacc gagtcacagt tgaaggagtt acaagtacaa
    11221 ctataaacat atcagcagaa ataacataca aaaatggcta cacttgggag aatataaaat
    11281 caattgcaga agaagcaata gacgactatt taaatgaact taacatgagt tgggaagatg
    11341 aagaaaactt aatagtccgt atatctcaaa ttgaaactag attacttagt attgatggag
    11401 tattagacat tgcaaataca atgataaatg atgttaaatc taatctaaca atagatagta
    11461 acagcatagt agtgagaggt gaggtagttg gataaagaga ttaatctaat aaattacttg
    11521 ccacaaattc tacaagataa agaagaatat ataaaagtat ttaatgtaga aaataaagaa
    11581 ataaaaacac tacatgataa attaaaggac ctatcaaatg accagttttt agaggaccta
    11641 actataagtg gtataaaaag atgggaaaag ataatgtcta taactcctaa aagtaatgag
    11701 agtttagaag atagaaggtt taggattttt agtaaatata taagtaaatt accttactca
    11761 gagagatttt taaggaactg gctagataat gtagttggag aaggcaatta tgaattaact
    11821 attaataatg ctacttataa catacacctt gagagtgatg ctagaaatca agattggttt
    11881 gaggaagttc attcttttgt aagtaatatt aagccatgta atatgacttt agattacact
    11941 agagtgctta taagcaaaga caattatatg aattttggta taacaaccct aatgggtcaa
    12001 gaaataacta tatacccttg gagtccacca gatatagaaa cttatggaga aattgatgta
    12061 ttaactggca atggagttgg ataccaagag ataacaatat tttaggaggt gatatattgg
    12121 ctatagataa aagttattac actataatta cagatgtagg aaaagcaaag atagcaaatg
    12181 caagtgtcac aggtaataaa gtagggtttg taaaaattca acttggtgat ggtggaggaa
    12241 gtgagtatac tcctactgaa agtcagacag aacttaaaaa tgtggtatgg gaaggcaaca
    12301 tcggaaatac aaccacagat gaaactgcgc caaactgcat aatattagag agtttaatac
    12361 catcaagtgt aggtggattt atgataagag aaataggata tttagatgat gaaaataatc
    12421 taattgcaat ctctaaatat aaagaatgtt ataaaccttc tatagaacaa ggagcagttg
    12481 ttgatatgaa ggttaaaact gtgcttattg tatctaatat aaataatata gaacttaaaa
    12541 ttgaccctac aataattttt gctacgctca aagatataca ggatttagat gctaaaataa
    12601 gcaatgtaaa cactaaaatt gatacaacta aaacagagtt aacaagcaac atagaaactg
    12661 ctaagacaga gttaaacact agaattgaca cagaaaatga gaaacaaaat attaaaattg
    12721 accagctaat agcaggtggt tcaaatgttg catctactca aacaataaca attgatgatt
    12781 ggatagataa tcaagaaggt ggattcaaag caactgtaac acatggttta ttaacacaga
    12841 gaataactgt aagtattata gatgctacta caaaagataa tgtagtaccc gatttcacaa
    12901 ttatagacga taattctatt gaggttagaa gtggagtaaa agttgagtta aatgtttatg
    12961 tgattaatgg gaatgcagag actcatttta ttaatgcgac tgtagatgat aatagagtgt
    13021 ctgaaatgac tacttattca tcaaaaaaga tacatgaaga aattggtaag gtagcagagc
    13081 aactgacagg aattaatagt aatattatat cgactgtaaa taacgacata ctacctatat
    13141 agaaaggagc gtgaaaaaat ggcgttatca atgagttatt ttaatttacc tgacaaaaga
    13201 aaatatacta aaaatcttgc atttaatcca tttgcaggtg gaaggcaaaa ttttgagtgg
    13261 actggtggtg accatggact aaatggtgag tttaaagaaa cttgcttaag ttgcacttac
    13321 aatggttcaa cattaaattg gggttcagga aatgtttggg tcttggggga atatggtcaa
    13381 tatacattta cttataattg tgaatcaatg catgtggata cacaacaaaa atttccgtat
    13441 acttctaata gaataataac tataaaaggt agacctgtaa tatctggttc agatacttca
    13501 cttgggaata aaagaaaagg atttagtgta gattttactg taagtgatga tactccaaat
    13561 gttaatttaa tagtacgtgc ttatcttgat gataaattaa tacaaaacat tactcctgtt
    13621 gtacaaaatt caactttaac agcaacagta acagatagtc aactaaactc tttatccgta
    13681 gatggaaatc acaaactaaa aatacaatta aatgatggat atgataattt tgatagaatt
    13741 tttactttta aaaaaataga aaagggaatt gatatatcaa catcattagt aactgatagc
    13801 caagctaagt ttactgtaac aaaaatatac agtgaattga caaaaataga gtgttattta
    13861 gatgaaacat taaaagagac ttttactaca gacttgtatt cagaaaagac tataaactat
    13921 gaacttatag acaatgcaat acacacatta aaaatagttg taactgatgc agaaaatgtt
    13981 gtagaagaaa aagttataag tataagtaag aatataatgc cattgcaacc tgatgcaaca
    14041 ttacaagata tatcaactaa gttaacagag attgggcaag gcgttagaaa tggtaaaaca
    14101 agcattataa atactttagc attaaagaat atagatgcaa gtttaaataa tacactcgtt
    14161 gagttatcag agaaaattaa gggaggtttt gatagtggag acgctagttt acaagatttg
    14221 atgaatcaat taacacaagc taataatact atatcgcagt taaatactaa gtataaagtt
    14281 gctagtggta cagttacttc ttttgcggat agtactaaaa ttgcttatcc atatctaacc
    14341 gacaatgtta ctaaacctgg ttcttggatt aaagttagta atttaggctt taaacctaat
    14401 attttctttg ctgattttga ttactatgat gctgaatata aaaataatta taaacttttc
    14461 cttttcgctt gtaatggtgt tgccacacaa agaggtgttg atttttcaag tgttacatct
    14521 tttatcagaa aaagtggtga tgagtatttt catgctaatg gatggctata tagtaactca
    14581 gagggggatg tctattttaa taatactggc gttcagattc cagcttataa ctttgattca
    14641 actcaaaaac atacttataa atggtatgct attaaattta tttaaaagga ggaaaaaaca
    14701 tgaatgttcc aaaccgaata atatatgacc aaacaggtag aacaatcttt gaaacaggcg
    14761 aatcctgtgg agatgtattg ccacattata caataacgga attgcattat atagatattg
    14821 aatatggaag tatagattat acaaggaata gagttatagg tataaatata gaaacaaaag
    14881 aaccgatttt agaagaaata ccagtatata taacagatga ggaaaagaga atacaagagt
    14941 tagaaaatca attattaatt gcagaaaatg agaaagtagg aggattatta taatgaatat
    15001 aaataatgtt gtagtaagaa tattagcaga aagaatatta agtaagggat taaatcctct
    15061 aaaaaataga gaatttcagt tagatgacgt gactaacaca gagtacagaa aagctgtaga
    15121 ggattatatt ataaaaaata gtggagtagt agaaggagca gaaccaacta tatagagggt
    15181 tcttttttta ttgaaagaag gtgactaaat gacttttaaa gagttggtta ataaagttag
    15241 aaatcttgta ttagaagcaa agaatgtaac tatagaagat acagaaagta aatttacaag
    15301 tgaaaatgta gaaggagcat tgaaagaatg tatagataga gcagatgagg cttttcaaga
    15361 agccgatagt ggaaaaacac ttttatcaac tgctatcggc tctcctacta catcagaaca
    15421 aacatttcaa gattatgcga actatattac aggatttaag agcaatataa gcaatttaga
    15481 aactcaattg aaaagcaaat attctattag atatggtcct attgatggat atgatggtaa
    15541 tcctttttct gctaattttg gcaagagtgc aagttacctt attgtctatg tttactttag
    15601 aagaagtgta tattattata atcccagtgg tagttcttta ggaagtaata caggaggttc
    15661 tgaacgtgca tggattacta taaacagcaa taaaactggt ttttcagttc attcatatga
    15721 tactagttat gagtcatatc cttttacagg ttattatatt gcttgtttcg cataataaat
    15781 tatattatta aaactaggag gatgtatgga agaaattagc ataaatctac tatgtgcagt
    15841 agcaggagtt gtaatatcct acttagcatt tagaaataac tcaaacagaa agatacaaga
    15901 tgaaacagaa acaactacaa aattagaaca acaaataact tttttatgtg agaatgtaag
    15961 agatataaag catgatgtag caaagtttaa tactagtttc ttagatatta gtgaacgagt
    16021 tgcaaaaata gaagcaagta caaaacaagc acatcttaga attgatgaaa ttgtaaatag
    16081 aattggagga aaataagaga tggataattt aataagtttt ataccagagc agttgctaat
    16141 tttagtagct gctctctcta ttataggtaa aggttgtaaa aaatataaac aattagataa
    16201 taaatacatt ccagtagtgt tactgatact tggaataggt ttctcaatat ggatgctagg
    16261 attaaatcct gttgcagtct tacaaggtgt aatttgttgg ggagttgcaa taggtataaa
    16321 tcaaacttac aaacagttga aggatggtga aaagtaatga aattaacaaa aatattatta
    16381 ctcacaaaaa tttaaaaaat ttcattaaat agagtatact tttatagcta ataagtatat
    16441 aataatagta cagaactcgt aaagcattta tatgctcaaa tatacgggac attgattttt
    16501 gaaggaaccc gccaagcttc ttttatagct caaataggcg ggacagataa atttacccac
    16561 taatattaat tggtgggttt tattatataa aagtatatct atgaaaaggt gaaaacaatg
    16621 gttgaagtaa aagaagaaaa aacatttgat gaacaaatag atattttaaa aagtagagga
    16681 ttaataataa atgataaaga agatgctaaa tttgtattaa gtaatgtcaa ttattatagg
    16741 tttacagcat atcttctaag ttttaagaat gatgatggct catataaaga aggaactacg
    16801 tttgaagaag tttatgatat atataggttt aataaggaat ttaggatatt attaacagat
    16861 ttgttaggga gcatagaaat agcatttagg acatacattg catatacatt agcaattaaa
    16921 catggtgctt gtggatatct agaaagggag agtttcaaag atgaaaaatt ctatattaat
    16981 tttttgacag cattagagag agaaaaaagt aataattcag ataagctttt tattatacac
    17041 cataaagaaa aatatgaagg aaaacttccc atttgggttg caacggaaat aatgactttt
    17101 ggtatgttgt caaaattata ttcaaacatg ttgccagaag atactagata cataaaaaat
    17161 aatttgtgta gagtgaatac tttattagtt aaatcttggt tacaatcatt aacacaggtc
    17221 agaaatcaat gcgctcatta tggtagaata tataataata atttccgtat tataacaata
    17281 aaaaacgaat ataaaaagta taacttggat aataaaaaga tattttctta tatacttgct
    17341 atgaagcatt tgactatgga taaattaatt tggaatagtt tttttataaa acttcaaaag
    17401 ttaattaatg attataataa ttctatagac ttaaagctta ttggttttcc taataattgg
    17461 atagagatat tggctaaata aaatagttac tttaagaagt ttataaacac ttactatatg
    17521 taagtgtttt tttattgaaa agaaggagga aaataaataa tgaaaatatg tattacagta
    17581 ggacacagta ttttaaaaag tggtgcatgc acttctgctg atggagtagt taacgaatac
    17641 caatacaata aatctcttgc accagtatta gcagatacat ttagaaaaga gggtcataag
    17701 gcagatgtaa taatatgtcc tgaaaagcag tttaaaacta aagcagaaga aaagacttat
    17761 aaaataccta gagttaatag tggaggatat gatttactta tagaactaca tctaaatgca
    17821 agtgatggtc aaggaaaagg ttcagaagtt ctatattata gtaataaagg tttagagtat
    17881 gcaactagaa tatgtaataa gctaggtaca gtatttagaa atagaagagc taaattagat
    17941 aaaggattat atatcttaaa tagttcaaat cctacagcag tattaattga aagtttcttc
    18001 tgtgataata aagaagatta tgagaaagct aagaaactag gtcatgaagg tattgctaag
    18061 ttaattgtag aaggtgtatt aaataaaaat ataaataatg agggagttaa acagatgtac
    18121 aaacatacaa ttgtttatga tggagaagtt gacaaaatct ctgcaactgt agttggttgg
    18181 ggttataatg atgggaaaat actgatatgt gatataaaag attacgtgcc aggtcagacg
    18241 caaaatcttt atgtcattgg tggtgcagca tgtgagaaga ttggttctat gactaaagaa
    18301 aaatttacta tgataaaggg taatgataga tttgatacac tttataaggc attagatttt
    18361 attaatagat agattatata aaatatttta gtatttcttg aaaatcataa gtatggtaat
    18421 atttagataa atattattta ttattaatag gtatgggggg agattatgat atttaagaag
    18481 tatataaaat cattactgat agttgttagt gttacattga taatgaacaa atcaatatta
    18541 tttgtaaatg cattagattt agagagtaac aataacataa aatcttataa aagaaagaat
    18601 gtaaaggaaa ttgatataag caataaattt gttgatgaat cctttagaag agatattgtt
    18661 gaaaaggtat tagataatag ggatgatcca ttaacaaatg aagttgcatc aaaagataac
    18721 tataaaatat atgaaagtga tttaaataaa attgagagtt gcttagagtt agatttacat
    18781 aaaaatccag atgtttctaa tccaaacaaa atagataatt tagatgggat agaaaatttt
    18841 agaaatctag aaaagcttga ttgtagttca aataatctag aaagtgtaga tttaagtaaa
    18901 aatataaaat taaaatatct agatattagt ggaaataaaa tattaaacat tgatttaaaa
    18961 aacaataatc ttattaatga attacattgt gatgcaaata aattatcaaa tttagacctt
    19021 aaaaataata aaaatttggt aagtttattt tttactagta atgaattaac agagatagat
    19081 ttaagtaata atgtgaactt agaaaattta caatttctta ataataattt aaaaagtata
    19141 gatttaagtc ataataaaaa gttgaaatct ctaaattgta ctaacaataa acttaaatcc
    19201 cttgatttaa gtaataatgt ttttttgaaa aatttaagtt gttcaggtaa taacctgagt
    19261 aaattaaata taaaaggact tacaagtctt gagtatttgg gatgtgctaa gaataaatta
    19321 caagatttag atgtatctac aaatataaaa ttagattctt tactttgtgg gaaaaatagt
    19381 ttaacaaaaa tagatataag taaaaataaa gaattaaatt cactggaatg tagtggtcaa
    19441 tatatagaaa aaagcattat aaaaaatggt gaaaatatag ttgtagatat aggaattgaa
    19501 aattcgaaat taaaggaatt atatccacaa aattataata tttcaacagg ttttctaaca
    19561 tgggaaaatc tttctgactt tccaaatagc ataagttata gtttggaata tgaatatggt
    19621 ccagtaaatg atacagagac tatggatgta aatatatcaa tagataaatt agtgaaatgt
    19681 agaatcagat atatagatta tgatggaagt ataattaatg aaaaagaaat atcagataat
    19741 tctagtataa atgaaattca aattaatcct caaagagaaa actatgagtt tagtggttgg
    19801 aggaacaata aagatgaaaa aatatataca tcagagcagg taagtaaatt agtttttgat
    19861 gatagtactg attttatagc aacttataaa agaaaatcaa caggatcaag tggttcttct
    19921 ggtggaactt caagtaaaga agattttaat atagcaccat ctaaggaaaa aataacaggt
    19981 gcagacagaa atgaaacatc agtgaaaata agtcaaaaag gatggaataa agctgataat
    20041 atagtgttaa taaatgactc tagcatatcg gatgctttat cagcaacacc atttgctaag
    20101 tctaaagatg caccaatatt acttactaaa aataataatt taaataaatt aacagaaaaa
    20161 gaaataaata gattagaagc aaaaaatgtg tacatagtgg gtggattaaa gtctgttgat
    20221 gaaaaagtag tgtctgattt aaagaaaaaa ggattaaatg taattagaat atctggaaat
    20281 gatagatatg aaacatcaat aaaacttgca aaagaattag acaaaaattc taacctgtca
    20341 aaagtagtag tggtaaatgg agaaaaggga ctagctgatg cagtaagtat gggagctata
    20401 tcagcaaaag aggaaatgcc aattcttctt actaatcaaa atgatgatat gaaagatata
    20461 aaagacttaa tagctaataa aaacatatca aaatcatatg taataggtgg agaatcttta
    20521 tttaataata aagaagtgaa taatacatta ccatctgtaa ctaaaatagc aggttctgat
    20581 agaacagaaa ctaactcaaa agtaataagt catttctata gcaaagatac acttaatgac
    20641 ttatatgtag ctaagaatgg aatgaataaa caagacgatt tagtagatgc cttatcagta
    20701 ggagtgcttg caggtaaaac tgaatctcca gtagtattag ttggaaatgg attagatgat
    20761 agccaaaaag agttaatcaa gaataaaaag tttaaaaata taactcaaat tggtggaaat
    20821 ggaaatgaaa aagcatttaa tgaagtagaa aacttagtta aatagattga aaggtagtaa
    20881 ctggaaatag ttactacctc cttttttatt cattttcagt tttttcaata attatttttc
    20941 catcctcaaa agtaacaaga acatcccttt catcaggaga gatattcatc tctttaatcc
    21001 atgacattgg aagtatcaat ctgggtgtaa aagagccttt gccactttta ttaaatccaa
    21061 ttctaagttt tctttgctcc aataatatca ttcctttcta ctattaataa atgccagcaa
    21121 taataaagta aataccaaag caataagagc tttaaaagta aaacttatta ttatattaat
    21181 aa
//
LOCUS       DAELSY010000064        21132 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.064, whole genome shotgun sequence.
ACCESSION   DAELSY010000064 DAELSY010000000
VERSION     DAELSY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21132
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.064"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            167..2479
                     /locus_tag="KPG09_002063"
     CDS             167..2479
                     /locus_tag="KPG09_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0966673.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKENNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDTTSNKVNYISDELD
                     TTSNKVNYVSDELDTTSNKVNYVSDELGMALHKVNCVSDELDMTSNKVNYVLDELDIT
                     SNKTDYISNELYNLSNKSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIKFKVCF
                     DMFMTDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFME
                     LAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNLVAWEDKKFET
                     PSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKK
                     SIAQAYINQRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHG
                     NPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            2567..2902
                     /gene="spoIIAA"
                     /locus_tag="KPG09_002064"
     CDS             2567..2902
                     /gene="spoIIAA"
                     /locus_tag="KPG09_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBG0966674.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            2916..3344
                     /locus_tag="KPG09_002065"
     CDS             2916..3344
                     /locus_tag="KPG09_002065"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBG0966675.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            3367..4131
                     /gene="sigF"
                     /locus_tag="KPG09_002066"
     CDS             3367..4131
                     /gene="sigF"
                     /locus_tag="KPG09_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBG0966676.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            4411..4848
                     /gene="spoVAC"
                     /locus_tag="KPG09_002067"
     CDS             4411..4848
                     /gene="spoVAC"
                     /locus_tag="KPG09_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBG0966677.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            5002..6015
                     /gene="spoVAD"
                     /locus_tag="KPG09_002068"
     CDS             5002..6015
                     /gene="spoVAD"
                     /locus_tag="KPG09_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBG0966678.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            6029..6391
                     /gene="spoVAE"
                     /locus_tag="KPG09_002069"
     CDS             6029..6391
                     /gene="spoVAE"
                     /locus_tag="KPG09_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBG0966679.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            6574..7590
                     /locus_tag="KPG09_002070"
     CDS             6574..7590
                     /locus_tag="KPG09_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0966680.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFVGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDRSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            7965..8807
                     /locus_tag="KPG09_002071"
     CDS             7965..8807
                     /locus_tag="KPG09_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG0966681.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            8822..9286
                     /locus_tag="KPG09_002072"
     CDS             8822..9286
                     /locus_tag="KPG09_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966682.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIITCFGQMIALKTQAQDLLIGSISG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            9273..10445
                     /locus_tag="KPG09_002073"
     CDS             9273..10445
                     /locus_tag="KPG09_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0966683.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGKVALIKAIKVLAMCGVDILYSK"
     gene            10489..11424
                     /locus_tag="KPG09_002074"
     CDS             10489..11424
                     /locus_tag="KPG09_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBG0966684.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(12223..12621)
                     /locus_tag="KPG09_002075"
     CDS             complement(12223..12621)
                     /locus_tag="KPG09_002075"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBG0966685.1"
                     /translation="MKWKELYNNARGKLNPRTISPFIDAGGVAAAILTDSGNVYTGVC
                     IDTACTLGMCAERNAIANMITNGESKITKLVCVMSNGSVGSPCGACREYLMQLDKDSA
                     CIEILVNVETEKTVKLSELLPDWWGSDRFI"
     gene            complement(12920..15613)
                     /locus_tag="KPG09_002076"
     CDS             complement(12920..15613)
                     /locus_tag="KPG09_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG0966686.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQST
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANQG
                     VYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGGGTPAT"
     gene            15827..16525
                     /gene="yunB"
                     /locus_tag="KPG09_002077"
     CDS             15827..16525
                     /gene="yunB"
                     /locus_tag="KPG09_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBG0966687.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(16737..17744)
                     /locus_tag="KPG09_002078"
     CDS             complement(16737..17744)
                     /locus_tag="KPG09_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG0966688.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNSEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISNLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            17972..18856
                     /locus_tag="KPG09_002079"
     CDS             17972..18856
                     /locus_tag="KPG09_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0966689.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDKKNKIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            19058..20608
                     /locus_tag="KPG09_002080"
     CDS             19058..20608
                     /locus_tag="KPG09_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG0966690.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKEKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            20637..>21132
                     /locus_tag="KPG09_002081"
     CDS             20637..>21132
                     /locus_tag="KPG09_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBG0966691.1"
                     /translation="MKKFLNVLFSVCISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSEDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVFLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFN"
BASE COUNT         7882 a         2656 c         3697 g         6897 t
ORIGIN      
        1 ttagtatgca aaattctgtg aagcaggaac actgtgactt tagtcatggg aggttcagaa
       61 aatatagcta tgttttatat actgtagttt tgaacataga aagtttaata aattatagta
      121 tatattaatt tttaataagg taagaaagta gaaagaagga tattttatgg atataataaa
      181 aaaattaagt catggatgta cattagattg tcatgattgt tgtaaattca atgtttatac
      241 taaagaaaat aatgtagtaa aaattgaagg agataaaaat catccttata caaaaggttt
      301 tatatgcaaa aaagggatgg ctcatttaga tagattaaat cataaagata gaataaagac
      361 tcctatgctt aaagttgatg gagtatggaa agagatttct tttgataagg ctatagaaat
      421 aatggcagaa aagcttacat actataaaga aaagtataca tcaaagtctg tcatgcatta
      481 tgaccaatat ggaagtgggt ctgtgctaaa atacatagga gatatatttt ttaattttta
      541 tggaggtgtt agcagacata agggtggtcc ctgttggagt gcagggatgc atgctcaaaa
      601 atatgatttt ggagtagcta agagtcatgc tatagaagat atgttaaata gtaaaagtat
      661 atttgtgtgg ggaaaaaatc cagcatatac tacaattcat actatgcaaa taattaaaaa
      721 agctaaagaa aaagggataa agatagtagt gattgacccc atatatacaa aaacagctca
      781 aatagcagat aagtatgtac aggtaaatcc tggaacagat ggagctttag ctatagctat
      841 ggcaaaaatt atagtagaag ataaattata tgatgaagaa tatataaact cgtatgttat
      901 tggttttgaa gaatataaaa aatatttatc ttcattggaa ttaagttttt taattgatga
      961 atgtggtgtt aaagagaatg atataagaga gttagtggat ttatatacta acaaatattc
     1021 aagtataaat gttggatatg ggcttcaaaa atataaaaat ggtggaaaca caataagagc
     1081 gatagatgct ttaggtgcta taactggtca aataggattt agtggtggag gggtaaacta
     1141 tgctaataaa gtttatccaa gtgttataaa ttctgaccct tacaatagcc aatcatatgg
     1201 tgaagatagg gagttttatg taagtaatat cagtaaattt atagaagaat ctttaaaaaa
     1261 tacatcaaat aaagtaaatt atgcatcaga tgaacttgat acaacatcaa ataaagtaaa
     1321 ttacatatca gatgaacttg atacaacatc aaataaagta aattatgtat cagatgaact
     1381 tgatacaaca tcaaataaag taaactatgt atcagatgaa cttggtatgg cattacataa
     1441 agtaaattgt gtatcagatg aacttgatat gacatcaaat aaagtaaatt atgtattaga
     1501 tgaacttgat ataacatcaa ataaaacaga ttatatatca aatgaattgt ataatttatc
     1561 taataagtca atcaaagata atattcctat aaagatggct gtgataacta aaagtaatat
     1621 gctgaatcaa ctgccagatt tagtagaatt ggagagggtg ttttcaaaaa ttaaatttaa
     1681 agtatgtttt gatatgttta tgactgatac agctacttta tgtgacatgt ttataccgtg
     1741 tacaaataca ctagagagtg aagatataat ttatagctca atgaccaatc catatataac
     1801 ctataatgaa agagctgtaa agccagcaca taaattgatg gatgaatatt atttctttat
     1861 ggagctagct aaaaaaatgg gattaaatga ttatcctttt gtagaaaaga gaacttattt
     1921 agaaaaagtg attgaaccac ttaagcgttt tgacaaaaat ctggatatag aaaaacttaa
     1981 aaacaattac tttactatac ataaccttgt tgcttgggaa gataagaagt ttgagactcc
     2041 atctggaaaa tacgagcttt attcagaaag tataaaaaac ttgggaataa gtccaacacc
     2101 tgtatatata agcaataaat ataaagaaat agaagataaa aatatttctt ttagattatt
     2161 aactaatcat catgcagata ctttatttag tcagcatttt atggacaaaa aatctatagc
     2221 tcaagcatat ataaatcaga gaatggctaa aaaagtaggt attgaggaca aagatattgt
     2281 gatattgagg tcaaaaaagg caaaaataaa tgtacaaata aatatagatg atggagtagg
     2341 taattatata gtaaaaatgt atgttggttg gtggaagaaa catggaaatc ctaatagttt
     2401 aacagacact ggtatatcag attttggagg tcaggtaaca tataatgaaa gtatggtaga
     2461 aataatacga caaaactagt taaatttcat tgaaggaata aaaatataat tatagaattg
     2521 attaaaagtt actattttta caaaactatt gaaggaggga tttttgatgg taaattattc
     2581 cttagaacat aagaatctat acatagaatt tatgtgctca gaactagacc accatgtagc
     2641 caatgaaata agggaagaaa tagataactt attaagtgta aatcaagtaa aaaatgttgt
     2701 atttaatttt gaaaatatta attttatgga ttcttcaggt ataggcgtca tcataggtag
     2761 atataaaaag atttctaatg aagggggacg agtttcagta attaatataa gttcacgagt
     2821 taagaagatt tttgatttat ctgggttaaa taaaattatt ggcatttatg atacgtatga
     2881 agaagctcta agttctttgt agggaggagt atgtgatgaa taatattatg gaagttaaat
     2941 tttcggcaat atctgaaaat gaaagtttag caagagttat agtagcatcc tttgcagcta
     3001 agttagaccc aacagtagat gagttagtag atataaaaac tgctgtctct gaggctgtta
     3061 cgaatgctat aatacatgga tatgaagaag acagttctaa gtttgttttc ttaagatgtg
     3121 agatagaagg aaatactata aagattatag ttgaagatga agggtatgga atagaaaatg
     3181 tggagaaggc aatggaacca ctttatacat caaaaccaga acttgataga tcaggaatgg
     3241 gattcacagt aatgaaaagc tttatggacg atgtagaggt aagttctgta aaagataatg
     3301 ggactagaat tgaaatgact aaaaaaataa atgtgcctaa ataaagtcca aaagtgaggt
     3361 tttattatgg aagtaactgt tgccagagaa gaaaaaaaac ctcttcttag tcatgaagag
     3421 actctagaac ttattgaaaa agttcaaaat ggagatgaag aggcaaaaga aatattaata
     3481 tcaagcaatt taggtctagt taggagcgtt gtatctagat ttttaaatat aggatatgat
     3541 agagaagatt tatttcagtt aggttctata ggcttaataa aatcaatata taaatttgac
     3601 ccaaaattta atgtaaaatt ctctacttat gcagtaccaa tgattttagg tgaaataaag
     3661 agatacttaa gagatgatgg tatgataaaa gtatcaaggt cgttaaagca aatagctgta
     3721 aaggcaaaga tggaaagtga agctttaact aaaaaattag gtagagagcc aagtatagaa
     3781 gaattagcta aagcaataga tgtagaaaaa gaagatttag taatggctat ggaagctaat
     3841 ttcaatgttg agtatttgca aggagttatt catgaagagg aaggttctcc aatatgcctt
     3901 attgataaaa taagcatgaa aggagaaagt gaagaagaaa aagtagtgga taacatattg
     3961 ttaaaagaca tacttggaag actggataag agagagcgtc aaataatagt tctcagatac
     4021 tttgaagaca tgactcaaag tgaaatagga gagatgctca atatatcaca agttcaagta
     4081 tctaggatag agaagaaagt attgtctaag ttaaaagaat atatatcgtg ataatactta
     4141 tgatatgtag aataacaaaa tataataaat atattactaa aataaaatgt aataactttt
     4201 tatgttaagt ttttaaatta ttaaaagata ctaaaataat aaaaagcatt aaaaaagtaa
     4261 aaatataaaa acaaggtgtc tttatggaaa aatagatttt ttctttatta ggcatctttt
     4321 ttattttttg accaaacttt agatagtcaa aataaataat aggtttattt ttttaaaatt
     4381 aacaaataat actttagaga ggtgtttagt atggataaaa attataaaaa atatgtagac
     4441 caaataagcc caaaacctac atatttaaaa aactatacac ttgcatttat tgtaggtgga
     4501 attatttgta tgatagggca agcaataaat gatttgtata tgaaagttgg aggtcttgat
     4561 aaactaggtg ctagttctgc aacatcaata acattaatat ttataggggc atttttaact
     4621 ggattaggag tttacgacct aataggaaaa agagctggag ctggttctat aattccaata
     4681 acaggttttg ctaactcaat agtatctcca gcaatggagt ataaaagaga aggctatgta
     4741 ttaggtgtag gtgcaaactt atttaaaatt gcaggaccag ttttagttta tggtataggt
     4801 tcttctattt tatgtggaat tatttattat atttttaaga tgttttagat gatttttatt
     4861 taatttaaat tttattaatt atgaagaagg tataaaatct gaaaatagct actaaagtaa
     4921 ctaattgaaa aataattgtg cagatttaat taatattaaa aatttgaata gtaaaatgca
     4981 aaattaaaaa ggtgtgaaaa tatgaaaaat aaaagaattg gaaaaagaac agtcaagtta
     5041 gaaaataaac caaccataat atcaactgga actatagtgg gtcctaagga aggtgaagga
     5101 ccactaaagg actattttga tatgattatg actgacgatt tatatggtga aaagacttgg
     5161 gaattagctg aaagtaaaat ggtagagact gcatcccaac aagctatcca aaaggcaggt
     5221 aaaaaattgt cagatgtaaa ttatatgcta ggtggcgatt taataaatca aatagtgcca
     5281 gcatcatttg cagcaagaga actggctatt ccatttttag gtatatatgg agcttgttct
     5341 acaatggcag aaggtctttg tataggttct atgcttatag atggtggttt tgctgattta
     5401 gtactgtcag gtacatcaag tcactattgt acagcagaaa gacaatttag attcccactt
     5461 gaacttggaa atcaaaaacc aatgacagct cagtggacag ttacaggagc aggaagtgta
     5521 ttattagctc ctaatggaga cggtccaaaa gtaaagtatg ttactgttgg taaagtaata
     5581 gatgagggta ttgatgatgg taataatatg ggaccagcta tggctccagc agcaatagat
     5641 actatctact cttactttga agatactaat gatgacccaa atagctttga tataattgct
     5701 actggagatt taggtactct tggaaaacag atagcagaag actttctaaa agaaaaaggt
     5761 gtagatatat caaaagttta tactgactgt ggtatagaga tgtttaacct taaagaacaa
     5821 gatgtccact gtggtggaag tggatgtggg tgttcagcta cagtgtttgc aggatatata
     5881 tatgataagt tgagaaaaaa agaatttaac aaagtaatgc ttgtatcaac aggagcacta
     5941 ctttcaccga caagtacact acaaaagcag acaataccat gtgttgcaca tgcagtagta
     6001 atagtaaatg agtaggaagt gatataatat gttaatggac tatgtaagag tatttattgt
     6061 aggtggaatt atatgtttaa tagcccaagt aatgatggat tatttcaaaa tgcaaactcc
     6121 atatgttatg gtaacgtatg taacaactgg tgtagtttta acttttctag gactttatga
     6181 gccattagtt aagtttgcag cagcaggtgc aacagttcct ataataggat ttggatattc
     6241 acttgctaca ggagtaataa aatcaattca aagtgatggt tttttaggta tttttttagg
     6301 aggtacaact gctacagctg gtggaatagc agcagctata ttctttggat atattatgtc
     6361 agtagtattt actccaaaag caaaaccata aatctttatt aaaagaataa taagtttttg
     6421 aaataataac tttttataaa gcaggtggat atcttttaga ttccacctat tttttatata
     6481 aagaagcgtt tacataattg aagttgacaa ttatttacaa catagtgtag tattatatta
     6541 tcaaaataaa atgaagtgaa gtaggggata aagatggtaa ctattaaaga tattgcaagg
     6601 aatctaggta tcagttattc aactgtttct aggtgcttaa acaataatcc taatgtatct
     6661 gaaaaaacaa aaaagaaagt agtagaagag gcaaatagac tagggttcca ttttaatgta
     6721 aatgcaagaa atttagcaaa aaaagaaact aacaggatag gggtaatctt ttcaaataat
     6781 tttaatcatc aagatactag aaagtttttt agtgatataa tggatagttc tataaattct
     6841 attgagacta ataaatacga ttttataata cagcctaata ataacattag tggtgatagt
     6901 aatgtatata aaatggtaaa tggacaaatg gttgatggat tagtaattgt gtctcaatca
     6961 ataaaaaaag aagagtatga ttttttaaaa gacaataatt ttccacatgt atttatatat
     7021 tttaaaccat cttttgtagg agaagtagac aatttctttt gggacgataa cgtgtatgga
     7081 ggttatatag ctaccaagca tttaattgac catggtcata aagacataat aacagtaact
     7141 tcagatgata ggagttcaaa aatgcatgaa gatagaacta agggatattt aaatgctatg
     7201 gaagaggcaa atttaaagac cgaagtcatt aaatctaaaa tggattttga atctcaagtt
     7261 gaatttttag aaaaatatat tgataaaata aaaaaagctt cagctatatt tgtacaacaa
     7321 gatgtacctg cgatttctat aatacaagag ttaaaaacta cttatggtat aaatgtacca
     7381 gaagatattt ccataatagg gtataataat attgaattaa tatcatattt taggtcgcat
     7441 ttatcaacaa tagatgaccc aagagagcaa gttataaaaa atggagtaga tagtttagta
     7501 aatataataa ataaaaagag tacagaacac attccaagaa aattatatcc aaggttaata
     7561 atacgaaata gcgttaaaag aataaagtag taagctttta agtatcaaaa gtttattaaa
     7621 aatattatag attatgtgaa gttttttaac aaacaatata aataaaaatt aaaaggagta
     7681 ggtgtatata agtacattgt actcctttat ttattgagat agtttttata atatgaatga
     7741 ataattttat tcaattattt gaaaatccgt tattttaaca atataaatta ataaattatt
     7801 tgtattttat atcatgaatc tataatattt ttttgattca taactaaaag tatataaaaa
     7861 tgatgaataa atatatatta tacattatca atgataggtt atataataaa atcactaaca
     7921 attatattat aagaaaatta taaaaattac aaggaggtat agaaatgaaa gaaaaagcag
     7981 ggattagaaa tgccataata gcagccttta taatcacaat attggcagaa ttaattggac
     8041 ctatttcatt taaagttatg ggaataaatg taactttact tagtatacta tgggctatat
     8101 tcataggaat ggctgtttct cctcatttat tagggagagt tataccagcc ttaaaaaagt
     8161 ttatagggga taatgaaata aatgtatctc catatttatt atctttgaca ctatatccat
     8221 taggaataat gtttggaatt aatgcagggc ctaaagttgg tatattactt caagcaggac
     8281 cagcactttt attacaagaa tttggtcata tgggtacaat gttgatagca ttacctgttg
     8341 gagtagcatt aggtcttgga agaagtgcat taggtggtac tttttcatta tgtcgtgaca
     8401 cagcactagg cattattgga gataagtatg gattaaactc accagaaggt ataggaacac
     8461 taggaactta cataagtggt agtatatttg gaacattatt attttcattt ttagcaccag
     8521 tgggtgtact tttaggattt catccatatg ctttagcaat ggcatcagga atgggtagtg
     8581 gttcaatgat gggagctgca acagcatcat taatgaatac ggttccacaa atgtcagagc
     8641 agattttagc atattcagct actagtggat tgattacagc tgttactgga atatatatag
     8701 aattattctt atctttacca gtagccaact actattatag caaagtagct ccagttattg
     8761 aaagatttag aggaagaaaa acaagaagaa caagtaataa attgtaaagg aggatatata
     8821 tatggaaaaa acttttaaga ctattatgga gcagataaaa atagtattgt ttatatatat
     8881 tattacttgc tttggacaaa tgatagcact taagactcag gctcaagatt tgttgattgg
     8941 aagtatatct ggatttttgt tggtgataat tgctattata ataaagaatt ttgttaaaaa
     9001 acctaattta ccaggttttg catgggctac attagttgca ttcgttttat cgcttccaat
     9061 atctccagtg ggggatatca tagcaacaaa tgtagggaaa atcaatttta tgtctacagt
     9121 aacaccatta ttagcttttg caggaatctc tgtaggtaac caattagata tattaaagac
     9181 tttgagttgg aagttagtag ttatatcatt tatagttatg acatctactt actttggttc
     9241 tgcttttata tctcaaacag ttttaagatt aaatggaatg ctttaaggag gtagtaaata
     9301 tgaatgaatt aaaagcaaaa attaaagagg aaataaagtt attatctgaa gagaaaaaga
     9361 catcatttga aaaagtttct gactatttat tttcaaatcc agaattagca tttgaggagt
     9421 ataagtcaca aaaagcatta tgtgatttgt tagaagaaaa tgaatttaat gtaactaaag
     9481 gtgtaggagg attagaaaca tcttttgaag ctgtgtattc aaatggaact aatgggaaaa
     9541 cagttgcttt tctagctgaa tatgatgcac ttccaggaat gggacatgca tgtgggcaca
     9601 atatcatagg gacaagttct gttggtgcag gtatcatatt aaaagaaatc atgaaaaaac
     9661 ataatataga agggactgta aaggtttttg gaacacctgc agaagaaaga gtaggtggaa
     9721 aaataactat gattaaagaa ggtgtattta ataatgtaga tgctgcatta atcttacatc
     9781 caagtgatgc ttcaatgcct gatgatatat catttgctca agttaattta aaatttgatt
     9841 ttactggtaa ggcatcacat gctgctgctt ttccttggga agggaaaagt gccttaagtg
     9901 gagtaatagc actatttaac agtgtaaata gcatgagatt acatcttaaa gattatgcaa
     9961 gagtacatgg aattataact gatggaggta gcatacataa tataattcct gaaaaatcta
    10021 cagctatatt taatgtaaga gccttaagca tagaatattt aaatgagatt tgtgaaatgc
    10081 ttaaaaattg tgctaaagga gctgcaattt ctactggaac taatgtagaa atagttcaat
    10141 tagatgaaat atacaaagag attaagaatg attctgaatt agtgaatata gttagacaaa
    10201 attttgaagt tttgggtgaa gactatgttg aaagagattt aagtcaaggt ataggttcaa
    10261 ctgatacagg aaatttaact catgagatac ctgcaataca agcatatata aaattaaaag
    10321 aaaatactgc aactcataca gatgaatttg ctgttgcagc tggtggtgaa gaaggcaaag
    10381 tagcacttat aaaagcaatt aaggtgctag caatgtgtgg agtagatatt ttgtatagta
    10441 aataatatat aattttaaaa tatattaaga aaggaaatcg gattagacat gatattaaaa
    10501 caagtaatag aggtttatga cattttagat aaagcaaatg ctaatgggga agaagtaaaa
    10561 acttacttac aaggttatgg tgaagtagat gtaactgtaa aagaattgtc ttcaagtaaa
    10621 ggttcaacag atttagtaaa gcttacaatt cctgggaaaa atggaaaatt aaaaggtgga
    10681 gatgctccta ctcttggtat tttaggtaga ttaggaggga ttggagcaag accagaagtt
    10741 ataggttttg tctcagatgg tgatggagca ttggtagcaa ttgcagtggc tgctaaactt
    10801 ttggatatgc aacgaaaagg ggacatatta gatggtgatg ttgtaatttc aactcatatt
    10861 tgcccagatg ctccaactaa agaacactat cctactcctt ttatggattc tccaattgat
    10921 atgatgacta tgaatgaaaa tgaagttgat agtagttgtg atgcaatact ttctatagat
    10981 actactaaag gaaatagagt aatcaatact agaggatttg caatatcgcc aactgttaaa
    11041 gaaggctaca tattaaaaac tagtgataac cttttagata ttatgcagac tgtaacaggg
    11101 aaatctccat ttgtattccc tttgtctatt caagatataa caccatatgg aaacaatctg
    11161 tatcacttaa atagtatttt acaaccagca gtagctacag atgcaccaat agtaggagta
    11221 gcaattacaa cagagatgcc tgttgctgga tgtgctacag gtgcaactaa ttttagtgat
    11281 ttagattcag ctgggagatt tgcaattgaa gttgccaagc tatttggtag aaataagtgt
    11341 gatttctatg ataaagaaga gtgggaaatg cttattaaga gatatggcaa attaaataga
    11401 tttcaaacat ttggtataca gtaattagaa aaatattaag tttatataat ttgtgataca
    11461 actatttaag aagatataaa caattaggaa taaaagtaag aataaagcac ttagttaaaa
    11521 actttaaaaa aaaatattta tttgtttata taaaaaggag gattctggtt attgggattc
    11581 tcttttttat gaatacaaat aatatattgt aattataaaa aaaggagcgt ttcaaagtga
    11641 tttttttaat caccttgaga caactcctta tattttttat ttaaattcta ttttttattg
    11701 actgtatagc tattttcacc atcataaggt ggcatgacct tgaacagcgt ggttttgtaa
    11761 agttgggcac cacaaaacaa aaaatcaaca agcccttgtg tggtctgctg acgtgtttac
    11821 tatactatac tttcccaaaa accgcaagca ctatgggagg ctataaggcg gcgaccattc
    11881 aggggcgatc ccctcgaaac cgggataggg ttcccatgtg ggacataata aagagcgccc
    11941 cggaggtatc aactaccatc cggggcgctc tttattatgt cccgtgggaa ccatgcccac
    12001 gaagcagggc gtacaacgga cagataggtc ctaccctcct gacagactgg aatctcaatc
    12061 ccatcgaaaa caatcataac aatcatggca agattatata cactcctgta aaatattttt
    12121 gtgtgtacca tgaagtactg cttgccacta ttgagaacgg ataaaaggga agtacgtatt
    12181 ataccaataa ggtgaacgaa acaaaatact catttgataa agttaaataa acctgtcgga
    12241 accccaccaa tccggcaaga gctcactcaa tttaacagtt ttttctgttt cgacattaac
    12301 taaaatctca atacaagcac tatccttatc aagttgcatt aaatattcac gacaagcacc
    12361 acatggtgag ccaacactcc cattgctcat cacacaaact aatttagtaa ttttactttc
    12421 gccatttgtt atcatattgg cgatagcatt tctttctgca cacattccca atgtacaggc
    12481 tgtatcaata cacacgccag tataaacatt tccgctgtcg gtaaggattg ctgcggcaac
    12541 cccaccagca tcgataaaag gtgaaattgt gcgtggattt aattttcccc tagcattatt
    12601 ataaagttcc ttccatttca tcaggttgat cctcctgttg tatccatata aataatcttt
    12661 tatacgcctt ttccgcctaa ggcacacaac acattactcg cttttttcat tctaatattc
    12721 gtgcgtgaaa atgtcaaacg gtttacttcg tttcattact tcatactgct gttcataacc
    12781 ctgtttgttc acttgggcac cattcgagca ccaatttacc cgtttcaggg ttgtagcgaa
    12841 cacagcagaa atgctcattt catgggcttt tctggctaga aaaaaaattt tatcgctgcc
    12901 agatatttac tattttttat taagttgctg gagttccacc accatcagga ggagtagtag
    12961 agccaccacc attatttcca ccattatcag gaggagtagt agagccacca ccattgtttc
    13021 cgccattatc aggaggagta gtagagccac caccattgtt tccgccatta tcaggaggag
    13081 tagtagagcc accaccattg tttccgccat tatcaggagg agtagaaccg ccaccattat
    13141 cacctgtgtt attattgtta ttattatttt cttctggttg gttattgtct ggtttttttg
    13201 tatcttcaga tgatggttga gaacttactt tagtaggagc agtaccttca ataaatgctg
    13261 ctttagagcc agaagattgc tttcctccat cctctaaatt aatttttgta aaatatacat
    13321 ttttaggaac ttcaaattca gtaaccgtta aattagcatg tattttttcc ataatttttt
    13381 cccatagttt tgcagtacta gtacttccat gtggtacttc tctacgtttt atagtgttac
    13441 cagaatcatc ttttctacca gcatcatctc caatataagt tgcaccaaca taataaggag
    13501 tgtaaccgac aaaccaagca tcatatgcac tatttgtagt acctgtttta cctgctacag
    13561 gcattccctt tggaagtgta gcaacaccac cagtaccttc agtaattacg ctttctaaca
    13621 tatctgtaac aacataagct acttcagggt ctacaacttt atgttcttca ggagcatttt
    13681 taactaataa ttgaccatca aaggtagtta tagtagtaaa aatagaaggt tctacataaa
    13741 caccttgatt tgccaatgta ccataagcag cagccatttg aagtggagta ataccattcg
    13801 ccataccacc tagtgtcaat gatgggaatt ttctatcact tgcttcagat gaacctgagt
    13861 gattattctt taaagttgtt ataccaaagt tctttaagta atccatcatt atatctacac
    13921 attcatcata agagtctcca agcatttcag cagttttgac agctatcgtg tttgaagatt
    13981 tagcaagtgc tttacgtaaa ctcatagaac catgaccagc agtagtagtt cttggattcc
    14041 atttattatt ttcttcaaac ttatatcctc ctcgagaatc acttagagca gttgattgtg
    14101 ttatttttag ggtatcaata gcaggtgtat atattgctaa aggttttata gaagaacctg
    14161 gctgatgagg gttagtagca cgattcaaag tttttctacc acttatattt cttcctccag
    14221 ctagtgctct tatttcacca tttttataat ctaagattac cattgcagct tgtggttggc
    14281 taactccatc agggccgacc atatgtcctg ggaaattgct atttctatca aattcttctt
    14341 ctaaagcatc ttgcatttta gggtctatag ttgtgtttac aattagacca ccattgtaga
    14401 ataagttttg agcttcatca tttgtatgtc cttgttccat taaagcattt ataacttcac
    14461 tttcgataag gtcttctaca gatgaagaaa ctttatctga tgattgaggc aattttattt
    14521 ctattttagc tttgattgct tcatcatatt cactttgttt tatatatttc aattctaaca
    14581 ttttctttaa aacaatttct tgtctttttt tagcttcagg gttacttaca gcctttctaa
    14641 caactaatgt acctgctttt aattgtctat aagtatcatt agatataagc tcccaagatt
    14701 ttattttttc aatcatatca aagtccactt gagtagggtc atctaaatca tcatcagttg
    14761 tgttaacaaa aaataataat ttattttcta aatcctcctt agtttcattt ccctctaact
    14821 tagaagtttt ataggctgaa aatcttgatg gatttttagt tgaaccagca agaagtgctg
    14881 actctgctag agtgaggtca gctgcagact tatcaaaata accacgtgca ccagcctcag
    14941 cacctgctag acctctacca acgaagaagt tatttaaata agtttctagt attttatctt
    15001 tacttactgt tttactcatc tcatgtgcat agtaaatatc ctttatttta cgaggtatag
    15061 tttgctgttc agttgttaac agcattttgg atacctgcat aggaatagta ctaccacctt
    15121 gcttagtacc tgtaagggtc tttaatactg acctaaataa acctttaata tcgacaccct
    15181 tatgctcata aaaacgctca tcttctattg caattacagc atgttgaaga taaggggaaa
    15241 tcttatctaa tggaacgggt gtctttttat ttacacttgg agctgtagac aatgtttttc
    15301 cattagcgta tttaatagta gttgtttggt tcgtctgttt atctaaaaca gcttctgtaa
    15361 ctggagttac atctcttagt gacgcaaata ctagaccagc agtacctgct gtaccaacaa
    15421 ctagtaaaac taaaaatact atcccaaata ctcttagttt tttaaattta tcgctttttt
    15481 tagatttttt cttgtttttt gttgaacgag tcttgttagc tgaagttcta ttaattggtt
    15541 tatttgtatt accagatgag ctaacttttt ttctcctgat tttatttcca tttttatcat
    15601 tatcgttatt cataagattt taacctcctt acttaatgca atattataac acaaatagga
    15661 gtagttttgt aattaataga aaaaatgaat ttgctataat attctgtaca ttgtatatgt
    15721 ataaacgtaa tatattaaaa agtctatatt tttattttat ttatttttga caaatattat
    15781 aaaaattatg ccaaagctac atataaataa ataggagggg ataattttga atagaatagt
    15841 agatagaaaa acaaaaaatg aatttagaaa aatcatagct atttttttag taattctttt
    15901 cttgtctgta tttataggca gttttatata tatagataaa actttaagac cgacaataac
    15961 agttttagca gagactaaag ctcttgaact agcaaatagg tcgataaata aagctgttgc
    16021 tgagatggta gaaggaaaaa taaattatga agatttaatg gatatacaac ttgataataa
    16081 tggtaaaata actatgatac aagcaaacac tataatgatg aatgaaattg catctgcaat
    16141 agctttagaa atacaagatg aattaaaaaa ggataagaca gcaagttctt acatacctat
    16201 aggaacagca ttaggaagtc ctatattagc taaatacggt cctaaacttg aagtgtctat
    16261 tgaacctata ggaacagtat cagtaaactt taaaacggaa tttgagtctt caggtataaa
    16321 tcaaactaga catagaatat atttagaagc tcaaacacaa gttaaagtag taataccact
    16381 tataacttca acaaaacaaa ttaaagctca aatacctatt tgtgaaacta taatagtagg
    16441 tgatgttcca gaaagttatg taaatattcc agaaaaaaat ctaggaaatg ttttacctaa
    16501 cactggaaag aataccaaca agtaatataa agttgtatat ttttaaataa attcaaaata
    16561 atagtttata aataaaattt ttggaaataa tattaatata attttaataa aaatttatat
    16621 tttatactat gtaaaatatc taatatattt ggtctgtagg ttaaataaaa gtacctttag
    16681 accaaatata ttattaataa cttaataaca tcttaaaact tatttattta tattatttac
    16741 aacatccatt taataaatac accataacct acagctgggt cattttcaat tgcatgtgac
    16801 actgctccga ttattttatt atcttgaact attggagtac cactcatacc ttgtacaata
    16861 ccaccagttt gagctaaaag ctttttatct gtaattttaa ttttaaaact tttactttct
    16921 ggatgttttt ggttttctat atctataatt tcaatattat attttttaca cttattaaag
    16981 ttatcttgta aaattatttg agcttttcct aatttaactt catctaaaga tgctacttta
    17041 tattttttta aattggaaat atcaaagtta ttaatttgac ctcttattcc aaaatcagta
    17101 ttttctgtaa agctaccaat aatattttct cgttttgcat ttatacatcc aacactacct
    17161 ttataagatt tttctatatt aaggtgctta gttgtagaaa ttgaacctgt tttaataggt
    17221 atttttcttg cattaccaac acttattgga tgacctacag ctccaaactc tgatgtatct
    17281 ggattgatat acgttaaagt agcaaatcca gaaagtaaat tgttaaaact aattttttct
    17341 aatatgtgtt tggtagtttt cattgttata agttgtccac ctctatttaa taaaatagat
    17401 acttgttttt cttctggtaa agcatttaag gtattggcaa agtcactata tccatcaact
    17461 tcagaattat ttacctttac tatagcatca cccaatctaa atggagatgc ttctccagga
    17521 tttctaacta tgatattttt taattctgcg tcaatttgaa gtacgtttcc aattggtatt
    17581 aaatattcag gttcattttt ttgtaaattt tgtgcataaa ttaaattatt tgagaaaaaa
    17641 tataataaaa ataaaaatgt taaaacaatt gtaaattttg acttagctaa gttggttttt
    17701 ttacttaaag tattattttt aaaatgaaaa tttttaagtt gcataaaaca cctctttctt
    17761 attaaattat tttaataata gaattgaagt tttcttgctg cttctatatt atgttgtgca
    17821 agaattatgt tttgatgctt ggaaaactga ctcaataaca ccttagtttt cataaaaata
    17881 aataaaataa atagtgtata tgataaaata aatacatatt catataaata aaaataaaca
    17941 ataactgtct agcataagaa aggagcaaaa aatgaaaaaa tatagattgt tagttacttg
    18001 tataattgcc atgggaatca taattatagg aacatcaaat ccagttagca atttttacaa
    18061 gactacaaat ttgattagtt caggaaatat cacaaatgaa aaagcagata aagatgaaaa
    18121 ttcaaacaaa aataataaag ataaaaatga agacaaatct aattctaaaa gtaataaaga
    18181 taagtcatca gaggacaaat cgtcagataa aaaaaataaa attcaaaaaa ataaaaaact
    18241 tttaatttgt atagacccag ggcatcaagg aaaaggagat agcaatttag aaccagtagc
    18301 tccaggttct tcctctaaaa aagcgagagt atcttcagga acagagggta ttgctacaaa
    18361 aaaaccagaa tatgttctaa acttagaagc atctcttgtt ttaaagagta tattagaatc
    18421 taaaggttat aatgtaataa tgactagaga gacacatgat gtaaatataa gtaattcaga
    18481 gagagcaata cttgctaatg ataaaaaagc agatatggtt gtaagaatac atgcagatag
    18541 tttaaataat tcttcaaaaa ctggagcatc tatattgatt cctgaaaaag atggaaaata
    18601 tacagctcct atatatgaag agagtaataa atgtgctgaa tttatcaaac agaatatgga
    18661 acaatcaggt atacaaatca atggaatcgt tcaaagaggt gatttgactg gatttaactg
    18721 gtcaaaagta ccagccgtat tagtagaaat gggatttatg agtaactata atgaagacca
    18781 aatgatgtca aatccagact accaaagaaa aatgatgcag tgtatagctg atggattgga
    18841 tgcgtatttt aaataagata tgaatagaga aaaagttagg ttggtattat tttcctaatt
    18901 tttatctcta ttttttattg tacataatgc aaagaagaag tttattttag gttaaattgt
    18961 cgtaaatata actaaaaata ttgggaatac ttgatttaag tttaaaaaga atatattata
    19021 gtatagaatg tgtaaaattt ttttgagagg tgttgaaatg ctagtagtag aaaatgtgag
    19081 tcatggattt ggtgcgagaa caatattaga aaatgtatcc tttagactta gaaaaggtga
    19141 acatattgca ctagttggtg ctaatggaga aggaaaatcg agttttttaa atataataac
    19201 aaagaagctt atgccagatg ctggaaatat aaaatggtct tcaagagcta cagtaggtta
    19261 cttagaccaa catacggtat taagcaagga aaaaactata agagaagtgc ttagagaggc
    19321 ttttaagcat atgtttgact tagagcaaga aatgattgct atgtatgaca aaatgggaga
    19381 agctagtgat gatgaaatga gtaagcttct tgaagaaaca gcagagatac aaactatact
    19441 tgaaaatagt ggtttttata tgatagatgc aaagatacaa gaagttgcaa atggtcttgg
    19501 tcttggagaa attggtcttg ataaagatgt aactgactta agtggagggc aaagaacaaa
    19561 agtacttctt actaaacttc tacttgaaaa tccaactatt cttatattgg atgagcctac
    19621 aaactattta gacgaagagc atataacatg gcttactaag taccttcaag agtatgagaa
    19681 tagttttgtc ttagtttctc atgatattga atttataaat aatacatgta atgtaatata
    19741 tcatatggaa aatggagagt taaatagata taaaggaaat tatgatgaat ttgtaagact
    19801 taatgatata aagaaacgtc aagaagaaca agcttatgat aaacaagttg aagaaagaaa
    19861 aagactagag gattttgtag ctagaaataa agctagagta gcaactagag gaatggctaa
    19921 cagtagacaa aagcaacttg ataagatgga aatattagaa agaccaaaag aaaaaataaa
    19981 acctactttt gcttttaaag atgctagagc agctagtaaa ataatttttg aaacagagaa
    20041 tttagtttta gggtatgatg aagcattgac aaaaccactt aatttccatt tagaaagagg
    20101 aaaaaagatt gccttaaaag gaatgaatgg tattgggaaa tctactctat taaaaacttt
    20161 acttggaata ataaaaccat ttgaaggaaa tgttaaatta ggtgactatt tggaagttgg
    20221 atattttgaa caagagagtt caagggaaaa tagtaataca cctatggatg aagtttggag
    20281 tgaatttcca gggcttacaa actttgaagt tagacaggct cttgcaaaat gtggacttac
    20341 aaatgaacat ataacgagtc aaatgagagt attaagtggt ggagaagctg ctaaggttag
    20401 actttgcaaa gtaatgttaa aaaatataaa tttcttagta ttagatgagc ctacaaatca
    20461 tttagatgtt gaggcaaaag atgaattgaa gaaggctatc aaagagttta agggaacagt
    20521 tcttttagta tgccatgagc cagaatttta ttcagaaatt gttgatgatg tatggaatat
    20581 agaagatttt accactaaaa tcgtgtagtg atttaactta ttgctggggg gatttcatga
    20641 aaaagtttct aaatgtactt ttttcagttt gtattagtat tgttataata gtaggtgtta
    20701 ttaattttac tgttggattt aaacaacttt attattttga cattgattac ttaaatatat
    20761 cagagttatc tggtttatct gaagatgata taaaattgaa ctacgattat ctaattgatt
    20821 ataacttaaa taagaatgtg tctgagttca agttaccaac attaaaatct tctccacaag
    20881 ggaagataca ttttgaagaa gtcaggaata tatttcaaaa tataaataaa ttagctaaat
    20941 tattattggt agttttttta gtaggaatta tattgagtgt aaaaaataaa aatataaaaa
    21001 tcttgaaaac tacatcaata acacttataa ttatgccatt attattaaca gttccaattt
    21061 tattaaattt tgaaaaatca tttattattt ttcataaatt attatttaga aatgattatt
    21121 ggatatttaa tc
//
LOCUS       DAELSY010000065        20694 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.065, whole genome shotgun sequence.
ACCESSION   DAELSY010000065 DAELSY010000000
VERSION     DAELSY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20694
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.065"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            159..1757
                     /locus_tag="KPG09_002082"
     CDS             159..1757
                     /locus_tag="KPG09_002082"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBG0966692.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFDVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(1992..2183)
                     /locus_tag="KPG09_002083"
     CDS             complement(1992..2183)
                     /locus_tag="KPG09_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HBG0966693.1"
                     /translation="MAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQ
                     SAGTAATLTIFRIADTVMT"
     gene            complement(2352..2945)
                     /locus_tag="KPG09_002084"
     CDS             complement(2352..2945)
                     /locus_tag="KPG09_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HBG0966694.1"
                     /translation="MRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYY
                     NLRTITWNDRVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNY
                     PYQIFEFTSSRDCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSIGPMG
                     ATGPTGATGNSSQPIANFLVNAPSPQTLNNGNAITGW"
     gene            complement(2946..3092)
                     /locus_tag="KPG09_002085"
     CDS             complement(2946..3092)
                     /locus_tag="KPG09_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HBG0966695.1"
                     /translation="MRKIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFF"
     gene            complement(3770..4294)
                     /locus_tag="KPG09_002086"
     CDS             complement(3770..4294)
                     /locus_tag="KPG09_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0966696.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(4359..5111)
                     /locus_tag="KPG09_002087"
     CDS             complement(4359..5111)
                     /locus_tag="KPG09_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG0966697.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDISKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(5142..6440)
                     /locus_tag="KPG09_002088"
     CDS             complement(5142..6440)
                     /locus_tag="KPG09_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBG0966698.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQQGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREVNVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(6457..8721)
                     /locus_tag="KPG09_002089"
     CDS             complement(6457..8721)
                     /locus_tag="KPG09_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBG0966699.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(8976..9794)
                     /locus_tag="KPG09_002090"
     CDS             complement(8976..9794)
                     /locus_tag="KPG09_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG0966700.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGIDEKIIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIIL
                     LDEPTAGLDPVSTRSIVDIIKGLNKDNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFVNENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(9787..10485)
                     /gene="cbiQ"
                     /locus_tag="KPG09_002091"
     CDS             complement(9787..10485)
                     /gene="cbiQ"
                     /locus_tag="KPG09_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBG0966701.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIKNIFVLSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEIFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKMHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(10488..10772)
                     /locus_tag="KPG09_002092"
     CDS             complement(10488..10772)
                     /locus_tag="KPG09_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0966702.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPDYEPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGVQKGKRS"
     gene            complement(10762..11514)
                     /locus_tag="KPG09_002093"
     CDS             complement(10762..11514)
                     /locus_tag="KPG09_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBG0966703.1"
                     /translation="MKQNIKLGVIAALMLIVLTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLVGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIISFLIFKALKKKDG
                     NNAMPVFLAAAIGDLATYTVTSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(11703..11895)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(12027..13799)
                     /locus_tag="KPG09_002094"
     CDS             complement(12027..13799)
                     /locus_tag="KPG09_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBG0966704.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYNMDKHPALDSMTRRIVNDIRNIDTN
                     QDMMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(13813..14742)
                     /locus_tag="KPG09_002095"
     CDS             complement(13813..14742)
                     /locus_tag="KPG09_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0966705.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            14971..15159
                     /locus_tag="KPG09_002096"
     CDS             14971..15159
                     /locus_tag="KPG09_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0966706.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRAKRRKEVQYE"
     gene            15152..15853
                     /locus_tag="KPG09_002097"
     CDS             15152..15853
                     /locus_tag="KPG09_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0966707.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLLDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSINNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            15855..16778
                     /locus_tag="KPG09_002098"
     CDS             15855..16778
                     /locus_tag="KPG09_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0966708.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            16935..17858
                     /locus_tag="KPG09_002099"
     CDS             16935..17858
                     /locus_tag="KPG09_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0966709.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGCEKRVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDHTLRLYNTTLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            17851..18588
                     /locus_tag="KPG09_002100"
     CDS             17851..18588
                     /locus_tag="KPG09_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0966710.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFASIALGRRK"
     gene            18599..19372
                     /locus_tag="KPG09_002101"
     CDS             18599..19372
                     /locus_tag="KPG09_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0966711.1"
                     /translation="MWKLLWAECQKIRRSKIVWITFFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            19388..19540
                     /locus_tag="KPG09_002102"
     CDS             19388..19540
                     /locus_tag="KPG09_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966712.1"
                     /translation="MKKIKKLIILIVFLFLFGMLHCYTEWKFNNDPPKGVIERINEWT
                     DKIGLD"
     gene            complement(19913..20521)
                     /locus_tag="KPG09_002103"
     CDS             complement(19913..20521)
                     /locus_tag="KPG09_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBG0966713.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         6821 a         3460 c         2618 g         7795 t
ORIGIN      
        1 taatttttat attaaatttg tagtatttat gtaagaaata aattttatat aatatttttt
       61 cactaaattt ttttcttttt acaacaaata ctcactacct tttgatataa tatttttatc
      121 aattattatt atattcaagt tttgaaagga gtattaaaat gagtttatta gttttcggac
      181 ataaaaatcc agatacagat tcaatatgtt ctgcaatttc attagcacat ttaaagaaca
      241 aactaggaat aaaagcaaca gcttatgctt taggagatat cagaaaagaa gctaaatatg
      301 ccttggatta ctttaaggta gatgcacctg aaattttaga taatgttaga atacaagtta
      361 gagatttaaa ttatgataaa gtagtacctt taacaccaac ttcatctata ttagaggctt
      421 ataaccttat ggatgaaaaa aatgttaaaa cactacctgt aacttatgat gatggaactt
      481 ttgctggtat cattactatg aaagaaatag ctaaaaatct acttcatcaa cactttacaa
      541 ctatacacac atcactatca aacatttgta aaaaccttaa tggtactatt cttgtggact
      601 gtggtgaaga tataaaaggt agagtagtca ctttatcatt tggaatggag actctaatag
      661 agactttaaa agaaggagac atagctattg ttggagatag atatgatagt attgaatatg
      721 ctattgatac aaaagttaaa cttttaatac ttactaatca tacagagatt tcaaaagatt
      781 tgctagctct tgctaagata aatggagtgt ctgttgtttc tgttgaaagt gacaattata
      841 aaacttctaa tgtaataaat caatgtagtt tcttagactc tatagaagct acagaaaatt
      901 taattacatt caaagaagat gattatatgg atgaaataaa agagattatg ctagaaacaa
      961 acttcagatc ttatccaata ttagatgatg ataataagtt tttaggttta gtttcaaaag
     1021 gtcatctatt aaacccatct aaaaagaatg ttgttttagt tgaccataac gaatatgctc
     1081 aaagtgctga tggaattgaa caagcaaaca tagttgaaat aattgaccat cataaacttg
     1141 gtggtatttc tactgatgtt cctatgtcat ttagagttat gcctgttgga tgcaatagta
     1201 ctataattta tcaaatgtac aaagaaaaca atgttgagat accttatgaa atagctgggc
     1261 ttcttttatc tgctatatta tcagatacat tattatttaa gtctccaaca acaactgata
     1321 tggataagaa agcttgtgaa gaattaagta aaatagcaaa agtagacatg gaaaaatacg
     1381 ctatggatat gtttaaatgc ggtacttctt tagatgaata tactatagaa gaaatagtaa
     1441 atatggattt taaagaattt aatatgagtg gacatagagt tggtattgga caagtattta
     1501 ccttagacat agactctata tttgctaaaa aagatgagtt tttatcttat ataaattcga
     1561 ctgattatga caaactaatt cttgcggtta cagatataat caaagaaggt tcttatttaa
     1621 tctataaagc agaagatagc ttaattgcta acgcatttga tgttgaaggt gttcaaggta
     1681 catttgcacc aggtgttgta tcaagaaaaa aacaattagt ccctaattta actactgcaa
     1741 tcaaaaattt taaataatat aaaattttta aataacgaaa actgtctcaa atctatattt
     1801 gagacagttt tttattatac atatattgat aataaagtta aataaaaatc cataaattta
     1861 aaatttttat gaatatattt tattattatt gttattttat tcattctaaa ataggtaaaa
     1921 ctcaatatat aaaaatactt ttgtcattta aataaaacct aaatagtttt taagattatt
     1981 ttagacacgt tttaagtcat aacagtatca gctattctaa atatcgtcaa agtagccgca
     2041 gttcctgctg attgcccatt tcgtatccct actgttgtag atgaaaaatt atttattgta
     2101 aaagtagtcc ccccaaataa aaaaccagca aaatatccag aaagtactcc tccgcctcct
     2161 ggctcagtag taagccctgc caaatctttt cctcctaaaa ttgggaatag gatagcaaaa
     2221 gaatattgat ttatacttga tgaacctggt tgtaatgcta ctgaaactga tatataatac
     2281 acaccatttt cttgtactgt aaacgtacca tttgcatcta ctgttatact tgaactattt
     2341 cctattattg tttaccaacc tgttatagca tttccattat ttagtgtttg tggagatggt
     2401 gcatttacga ggaagttagc aattggctga gaggaattgc ctgttgctcc tgttggacct
     2461 gttgctccca ttggtcctat acttcctgtc gctcctcttg gtcccattgg acctgtaatt
     2521 ctaattggaa gacactcaca tttaggataa caattatcta taattctatc ttcaaatgtt
     2581 acaagtatat acggtttgtt acaatcccta ctagatgtaa attcaaaaat ctgataaggg
     2641 taatttaaag ataaagctaa cccataatta ggatatttat tcattgccca tgcttttata
     2701 gttcccgtaa tatttaaaca aatatagttg gatgtatcac ttatcccaat tggtaaatat
     2761 cccctgatat tttccataga cactctatca ttccatgtta tagttcgtaa attataatat
     2821 tcacttattc ttttaatttc aatatctact ttttctttaa aaatatgatt cttatttgaa
     2881 tcaatatgaa taaataactc tgcttttaat atacttttgt cttttaatat atctggtatt
     2941 ctcacttaaa aaaaagtaat caacttttct tttacaaaac tatttgaaca tttacttcct
     3001 attaagcaat aatctaaatt actaaagttt ttatctggat attttttaga tataaaagta
     3061 tcatcattta aataaagtat aatttttctc atttataatc cctccatttt gggtctatat
     3121 catactatgt ttaaaataca tgcttagtta caatacaatt aaaatttata gtattaaatt
     3181 tttatataat aatgctgatt tctacaaaaa aagaacatgt cattagaaac ttctaatagc
     3241 atgttctttt tcattattat atattacact ttaaattatt ccttgactgg cttagctggt
     3301 gtaaactaaa ccagtctttc taaaatatta agcacttttt tcttattatg ttttttagga
     3361 tttattgtct ctattattgt aaaccaaagt tataaaattg taaatagaat atattaccat
     3421 aacaatattt ttttataagt aaaaattata aaaaacctta tttttattaa atagaataaa
     3481 taaaataagt ttaaaaaata ctgaaataag tgttttccac atattttata tatttattta
     3541 atacattatt atttaaaaat aaatatataa acaatcataa taaaattttt ttaataaaac
     3601 tttatttttt agttaaaatc atacaatata tagctttcca caatacaaat aaattaaaaa
     3661 gtgaaatgta tttgtctaaa aacataataa catttcactt tatcctcatt tttaagtaat
     3721 atatattttt aatttttatt tataactcaa tctagttttt atataagttt tagttttttt
     3781 ctacatctga gtaatctatt ccaaaagttt ctacagtaac agacttcata atttgtggag
     3841 tttgtggctt atcagctgaa tctgttctaa ctgatgctat ttcatcaact gtatccatac
     3901 cttctatcac tctaccaaaa cctgcatact gaccatctaa gtgtggtgag tttttatgca
     3961 ttatgaaaaa ttgagaacca gctgaattag gtgccatagt tctagccatt gatatgactc
     4021 ctctttcatg ttttaaattg ttagtaaatc cattaccact aaattcacct tttattgagt
     4081 gacctgggcc acccattcct gtaccatttg ggcatcctcc ttgtatcata aatcctggta
     4141 ttactctatg aaatattatt ccattgtaat atccttcatt tactaatgat acaaagtttt
     4201 taactgtatt tggagctata tttggatata actcaatttt gatttctttt ccattttcca
     4261 tttctatagt tactatagga tttttatttt ccataattta ttaccttcct tttcattagt
     4321 taaattacat aattacattt attatattat actctaaatt aaagtctatc aagtatattt
     4381 tttcctattt cgtatccttc ttgataaata gttttacaaa actcatcatc aaagttaagc
     4441 gtatctttag gagcaaattg agtattaggt ctaactacta ttattttatc ttcatcatat
     4501 acttttttaa tatcctctgg caactcataa ttatattttg tggatattaa aataatttta
     4561 tccacattat ccccaattaa tggttcaatt aatgcactcc taattaaacc tccatctaat
     4621 ttgaaaccat cataaattcc ttcttgcaca tctttcacaa attgttcttt aaatccaaga
     4681 gttgcgtttg gatttgatgg taacaaccca ctgtatgaaa tgctgataat ggcttcatta
     4741 cgaggcattt ttgatatgtc aacaatcttt tctgatacta ctttattatc aacttcaaca
     4801 taattaacat aaaagttcat ttcttctata ttactatcat ctagttccct taggttgtct
     4861 atagcaggag aattatccac agtattattt actattttta catttccatt ctggatatct
     4921 tttatatttg tccacatttt ctccaagtta tcaacattcc ctgtgtacaa ataatatcca
     4981 tttattgctc ctattgatgt ccctgaaatc gaataaaatt tatttattcc tccatcatac
     5041 aaggctttaa ctactcctgc ttgataagcc ccttttgctc ctccaccttc taaacataat
     5101 cctaatttca tagtctcatc tccaaattgt ttatcttata tttatatttc aaaaaactta
     5161 ctaaatgttt tatatatact atagtggtcc tcaacatttc ctaattctct tatagagtgc
     5221 attgctaaaa ttggacttcc cacatccaca gatggtatat ctatatgagt agaagatatt
     5281 ggacctatag tacttcctcc tctttcatct gacctattaa caaattcttg atactcaaca
     5341 ttaacttctc tacatatatt tttataaata gctatagaat atccatcact agtatatgcc
     5401 tgactagcat ttatctttat aactggacct tttcccatta ttggtttatt agttgggtca
     5461 tgcttttctg gaatgtttgg atgaatagca tgagctaaat ctgatgatat cataaaagat
     5521 gaataaatag actcaaagaa tccctctcta tccttaccca ttgatatgca aattctctcc
     5581 aatatattta acaacatgtt tgaatctgca ccttgtttag tagagctacc aacttcttca
     5641 ttgtcaaata cagccattac atttacacct tgttgacctt tagtattaac aagagcattc
     5701 aaactagcat gtaccattgc aagattatct tgtctcccaa tagatataaa ttcatcatta
     5761 gcccctatta aacttccttt ttcaaattca tataaaaata aatcaaaatc tataatatct
     5821 tccatattta catctaagtt agatgctatt tgctttataa gaaaattatc tttttctaat
     5881 gtatcattta acatcccaac taaaggtaac atatcttttt gcttattata tgaatgacca
     5941 tcatttatac ttctatttaa atgtattgct agatttggta ttatacaaat tggcttttta
     6001 aagtctataa gcctttcatt tggttttaat atactatcac cttttaaaac tactctacca
     6061 gctatcccaa gaggtctatc caaccatgta cttaatattg ctcctccata gcattctgta
     6121 tttaacttca aataagattt ttcagcttct atttctgcat ttggtttaat actaaatgta
     6181 ggagaatctg aatgagagcc tattattcta aatccatctc tttctatatc ttctgaattt
     6241 actacaaaag ctactatagc tgatagattc ttagtaacat aatactttcc accaactctt
     6301 agattccatc tttgattcaa aactaattct tcaaatccat ttttatctaa gatttcctta
     6361 gtactctcga ctgcatgaaa agacgacgga ctatcatata tgaagtctat taagttttta
     6421 gcaaagttta tactatccat tcctcattta cctccattat aaaatattta gtgttgtata
     6481 ttctttattt atttttttaa ttccctgttg gtcaaaagct atagtaacat ctgtaccaat
     6541 tatagaaacc actgtcccaa ccccaaattt aggatgatgt accttagaac caagttttat
     6601 atcatctgga ttagtttctt tattagaatc ttttattgta gcattaactt tatctgcaac
     6661 tttagatttg cttatagtac tcatatactt tttagtatat ttatctaaca tattataact
     6721 tgctttagaa tagcttagtt ctttttttac tttgtacaat ctttctatac attcttctgg
     6781 cagttcttcc ataaatcttg aagctattgc aacatttgtt ttaccatata atgttctctt
     6841 ctctgtcaat gacatatata gctcttcttt agctctagtt ataccaacat aacaaagtct
     6901 tctctcttct tctatttgtg aatcactcat agattttata gctcttgata taggaaataa
     6961 cccttcttcc atacctgtta aaaacacaac agggaactct aaacctttgg atgtatgaat
     7021 tgtcataagc gaaaccctat cattgtcttc ttcatcattt gattcagatg taagtgctat
     7081 acttgttagg aatgtctcta agtctttatt ttcataagta tcatcattac tactttcaaa
     7141 ctccaaagct atagaaataa attctctcaa attatcaatt ctgtcttgag cttcttcacc
     7201 tttgcctgtt aaatcttttt catttttatt tcttatctct acaagctcgt ccatatatcc
     7261 tgtagtatcc aagacttttt ctataagttt gctaacagga taaacttctt ttatagttct
     7321 aagagtccct ataatatcaa caaatccact tatacttgcc cttgccttag tagatatatc
     7381 tgaatttgtt tctatatcaa ttaatactga gtatatactt tcctgtttta agttagctct
     7441 atcttcaatt ttttctatag ttctaagccc tatacctctt ctaggaacat ttataatcct
     7501 ttttatagat atatcatctt gtggattttg aataactctt aaatacgcca acaaatcctt
     7561 tatttctttt ctctcgtaga attttgtacc accatatata ttgtatggaa tttgacttct
     7621 atttaaagcg tcctctacag gtcttgcctg tgcatttgct ctataaagta ctgcaaaatc
     7681 cttataaggt ctattttctt ttctagctat ttgtgcaatt gaatcagcaa tgaaatcagc
     7741 ctcttctatt tcgctaccag ttaattgtat ctttataagt tctccttctt ttttttcact
     7801 ccaaagcttt tttctttttc tttctatatt attagatata actttattag ctgcatctaa
     7861 tatcacttgt gtagacctat aattttgttc taactttaca acatgtacat tgtcataatc
     7921 tttctcaaat tcaagtatgt ttcttatatc tgcgcctctc catccataaa tactttggtc
     7981 atcatctcca acaacacaaa tattttgatg ttctcttgct aataatttta tcaattcata
     8041 ttgagcttta cttgtatctt ggtactcatc taccattatg tatctaaatc tacttctgta
     8101 atatgctaat acttcatcat tagccttgaa aagttctaca gttttaagta ttaaatcatc
     8161 aaagtcaagt gcactatttc tttttaaacg gtcttgatat aacgcataaa tatctgcaat
     8221 cttaaccatt ctattatctg ccatgttgat atctttaaac tgctttggag tataaagttt
     8281 gtcttttgct cctgaaattg cacttataac tgcttttggt tcaaacactt tatcacttaa
     8341 atttaattct tttagacagt ctttaaccaa tgtaacttgg tctgctgaat cataaattac
     8401 aaaacttcta ttgtaaccaa tcttatttat atcttttctt agtatacgaa cacagcaaga
     8461 atggaatgta cttatccaca tatccttagt ttcaggacct atattttgtt caactctctc
     8521 tctcatttca tttgcagctt tgtttgtaaa tgttatagct aatatatttg gtgcttgtac
     8581 acctttatct tctataagat atgctattct tgttgttaat accttagttt tacctgaacc
     8641 agcaccagcc agtataagta ctggaccttc agtcttttct actgcttctc tttgagcagg
     8701 atttaatgta tctaaattca tatttctcct cctgttaaca gccataaata tattttaatc
     8761 taaaatatga attacttcaa attataatta tgtattatat taatagatta tttccaatat
     8821 attacaatta aataacattt ttccatataa tttatttaat tgtttaataa aaaaaataac
     8881 cttatgattt ggggaatcat aaggcacctt taaggggtct aggttaaggg ttattatata
     8941 taaatctatt tttttaaaat aggtttaggg cttaattatt tatttgtatt aagttctctt
     9001 tccttccaat acttatacaa atcttcttct ttcttaaata agggtaaatt catatttcta
     9061 tgtactttta ctaaccatgg ggattctagg ccagcttgta taatgttatt ttcatttaca
     9121 aatacatttt ctgcctttcc ttcatcaatt aaaatcccct tatccaatac atatatataa
     9181 tcgcatattt catacattaa attcatatca tgactggata ttactatttt tatattatct
     9241 ttattcaatc cctttattat atctacaata gacctagtac ttactgggtc taaaccagct
     9301 gtaggctcat caagcaatat aatttcattt tccattgcta taactgatgc tatcgctacc
     9361 ctttttttct gaccataact taaaaaatgt actggtctat ctataaaatc tattccatta
     9421 acagctacta gagctttgtt tattctctct ttaattattt tttcatctat accaatattt
     9481 ctcattgcaa aggctatatc atcatatact tttgaataaa aaatttgttt ttctgggtct
     9541 tggaacacaa tacctacatt ttttctaagg ttgtataaac ttctcttatc atattttaac
     9601 ttttcttctt taaaaagaat ttctccagat gtaggcttta atatacccat taaattcata
     9661 aataatgttg attttcctga accatttgaa cctattatac ctataacatt tccctttgaa
     9721 aaatccatat taatatttaa tagtgcattg gtattctttt catattgata agttaaatta
     9781 tttattttaa acatcattat cccctactat gtgaaattta ccatcataca attttatgtc
     9841 taatgatata cacatatcat cataccttgt cataagtctt ttgtacagca tactgccaag
     9901 aattcctaat gatttatatg aagtttttaa atttatgtac ccaaatctta gttcttgtga
     9961 cttatatatt tctgaaaatt cttctaaaaa tatgaatata aacctatata ttaacattga
    10021 tagctcaaca aaagtatcag gcaaatgcat ttttttaaag aagaatatta attgattaaa
    10081 aggtgttgta agtatacaaa agtatacaca agtcaaacaa gacatagctc taaataacac
    10141 atgaatagac atgtcaatag atgcttttga tattcctaca ttgaaactaa aaatttcaaa
    10201 actatataat aagtctgcct tattaaaaga tatatttaca agagttattc ctatactaag
    10261 aaataaaaag tacattggta ttctgagtaa ttttaaataa ttctttaaat ctattccagc
    10321 tacacaaaca actagaatac tcattaaaat cataattcca cttaaaacaa atatattttt
    10381 tattaccata gaagcaatca gaaagattac tcctatggct acttttttat ttggatttac
    10441 tttacttaat ctatttgtat atgcatattt atcaattata agcataatta acttctcttt
    10501 cctttttgaa cacctaatat gtaccctatt acaccagcac ctaacgctgc ttgtgcagag
    10561 aataataaac tttctatttc tccacttggt ggctcatatg ggctactaaa ccatggttca
    10621 taatctggat ttatcttagt aatttcactc tcagcttgac catctgctcc tccatattca
    10681 gctcccgaat taacaagtaa tgggaatatg ataagagcta ctactaataa aagtagtaat
    10741 atattagttt tcgtctttgt tttagcactc attagcttta actcctttat cattattttc
    10801 tagtatgctt ttttctccat tttctgtaat taaattatat acaattacag taagaatacc
    10861 ctctgctatt gctattggta tttgagtcat aaagaaaatt cctaaaaact taattgctga
    10921 agccatcact ccacctgatg ggtctgggaa agccagtgct aactgtatcg aagttactgt
    10981 atatgtagcc aaatctccta ttgctgctgc aagaaatact ggcattgcat tatttccgtc
    11041 ttttttcttt aatgctttaa atattaagaa tgatataatt ggtcctataa tagccattga
    11101 aaaagcatta gctcctaaag tagttattcc tccatgtgcc aatagtaatg cttggaatat
    11161 tagaactata gttccaagaa caaacattac acttggtcca aacattattg ctccaagacc
    11221 tactccagta ggatgtgaac aacttcctgt tactgaaggt atctttagtg ctgacaatac
    11281 aaatacaaat gctccacaca gcgctaataa tactttcttt ttagggtctt gctttactat
    11341 ttttcctatt gattttagtc ctactaaaaa aaatggtata aatataactc cccatgctat
    11401 agaccatttt actggtaagt atccttccat tatgtgcatt gcattcgatg taactggagt
    11461 taaaacgatt aacataagtg cagctataac ccctaatttg atattttgtt tcattttact
    11521 atcccctctc tccctttcta ataattaaaa tataaataat atatgtctga tttattttaa
    11581 aaataaaaat aaaaaagcct aactcgatgg ctaggcttct aattttcgat aatccaaata
    11641 caaacacata aaatgaacat atctgtataa ttattataag aaactttctc catccccgaa
    11701 gaaattactt taatatttta ggcaggtctc ctgacttagc ttcttcctac tcctcttcct
    11761 tcccatgaga tataaaaaat atcatcatag tggctaacaa aagtttcgtc tgcatcacag
    11821 tagcgggggc tgtaaaggaa tttaaccttt ttccctatta atcttgctta aagaacctat
    11881 aatattatat atttaatttt taaattattc ttaaatcatt ttctatataa atcattgtat
    11941 accttttctg tgaaaatttc tacatttttt ataaatttta ttcattttta tatttttatg
    12001 tatataaatt cttttttgta agtttattaa tttctaatta tcttctttaa atacattgaa
    12061 actacatcag aaaatctagt tatatctctt gaatatataa aatcatttcc atatattaat
    12121 ctttcagctt caagttctga ttcttttcct gtaaatactc ctagtactaa tatattttgc
    12181 tttctagctt ttctcacttc attggctgta tctctcaaac caactatacc cctataagcc
    12241 ttttctcctc ttaatgttct ctcactactt tttcctatct ttacatcatt tggtcttcca
    12301 tcgctgagaa ctatcaggat tttattttct tcatctctat ttattagacc actacatata
    12361 gattttattg ccaaaccatc tctattatta ccagcacaaa aatattcaaa tatattttca
    12421 ctatttttta tactatcatc ataatcttta aatcttttta atattgtata gtctaaaaaa
    12481 ctagaaaatc ccatcactct atttggtatg ccaacttctg ttagagcatt tgcaattatg
    12541 taagcttgaa ttgccacatt tccctgattt ctactttgag agccacttgc atccaataat
    12601 atatcaataa cgtatttccc tttttcattt tctatatctt tataaaatat tttattatta
    12661 ttacttctac caactctcca agctctattt gctaatatag taccacaatc agaatatact
    12721 ctacttttag cattttcttc tattaaaatt cttgacagac tttcttttaa tctctttatt
    12781 tgctgtctat acattctagc atgttctcta aattttccta aattattttc tttttgtctt
    12841 gttacatatt ttagttgaaa tacattatta cactcacttc taagaactcc atctgtaaag
    12901 tgtattctac aaccttcatg aacatttcta caattttgtg tctcaatttt tttaatttca
    12961 cctttactca aataagtttt tccataataa tgttctattt ttgaatatat cttatttgca
    13021 gtgtcttcat caacatatat aactcggtct gctgaattgc tacatttaga atcatcaaaa
    13081 tcccctacac cttctacaag catggatgaa gatattgaat ctatatcact ttcaataata
    13141 ttttcttctt catcactata aagttcttca tacatgaaat cagcaaatgt atcaaaattt
    13201 acttctacac tttctacatt attttcatca ataagactat tttcttctcc atttaatatg
    13261 tattcgaaat aagttaaata tataacatct aatgctttta aaatctccat catatcttga
    13321 ttggtatcta tatttcttat atcattaact attcttctag tcatactatc taatgctggg
    13381 tgcttgtcca tattataaag tacaaatgtg tatttaattt tatccataat attatcattg
    13441 tgtattttgg taaaacgtga aacaatatct ttataagcat ttcttcttat gtcttctatc
    13501 cctggcctat ctttaatgac cttttcttca actattgtat ttaatataac ttgaattaga
    13561 cccagtataa tatctttgtt gtatcctttt cttattctat ttgtaagata ttttttaact
    13621 atatcccaat ctacatactt tcttctagca cctgcaatta ttgcaaaata tagacctatt
    13681 tcttttgaag agtaatcttt ttctgatata tctatacttt cattgtaatc accagctatt
    13741 gtccatgata agttattagc tctattttca aattcataat catcatatat ccatttcata
    13801 atctcaccta tattaaatat gacttattgg aaatacatct atactctccc atttattagg
    13861 tattctagtt ttaattacat ctccaaccat ttctttctca tatacatcaa aagtctttcc
    13921 tgtaagtccc atattaattg caagagttgg ttttaatcct cttcttatag tcttaagaga
    13981 tgccataaga ccacgtaaat caatagcttt actagatatc tctccatttt gagacttcaa
    14041 ttgtaaatct aaaaatattc ctgcaaagtg tattaatttt tcttcatcag catctgaaaa
    14101 ttcatttttt aaaatcatca ttaacttatc ttcttctata ggaggaatat ctattaccat
    14161 aaatctagat accagtgctt cattaagttc ttttgttcct gcatattcat aattcatagt
    14221 cccaataaat cttgttgctg gatgtagatt gactctctca tacccaggta catcaattat
    14281 tcttctatag tctaaagctg aatgtaccac aactatagca tcattttttg ccatattaat
    14341 ttcatcaaat actccaaaac ctccattaat agcacactca taaactgagc ctcttctaag
    14401 tttaacttca ttgtctataa atgtatctgt acctataagt gttgaactgt ctgtatttat
    14461 atgaaatgat gtattccact gaggtctatt aaatatttcc cctagattat cagcaagtaa
    14521 attttttccc gttgcctttg gtcccgataa taatatattt tcttcttcta aaatagctgc
    14581 tatacacata gacaagatat ctttaccata aaaataagtt ttagactttg ttactctgta
    14641 ttctaaatct ttttctaaac cataataatt tctaaaatac agtacatctt caataagttt
    14701 ttcatctacc ccttgcaccc ttagattatc tattatatcc atatttttac cccttttata
    14761 taaatagtat ttgtttcatt tcttatcacc attttagcat aaaaggatat aagctaaaat
    14821 tgatttttta atctccccca ttttaagtaa aaccatgcac cattttatca ctacaaaatt
    14881 aagagtacta cttactgata tttcttgatt ttgttcgtct atacgtatat aatatatgct
    14941 gataaattat gaggaaagga tgattgaaat atggaatata tgtcttgccc agaagtagca
    15001 aaaaaatggg gcatttctga aagaagagta caaaaactat gtgaagaaag tcgcatacca
    15061 ggagtttcaa aactcgggta tatgtggctg ataccaaaga acgcagaaaa gcctgttgat
    15121 ggtagagcga aaagaagaaa ggaagtacaa tatgaatagc gttttgatta tagatgatga
    15181 caaagaactt tgtgctctta tgaaaaaatg tgttgagcaa gaaaatttat ctgctattac
    15241 tgcacattgt ggtgcagaag gtctacattt gctcgataaa aatagacaca cactctctct
    15301 tgtaatttta gacataatga tgccagatat agacggtttt caagttctga aaaaaattag
    15361 agaaacaagt aatattccag taattatgct tactgctaaa aatgacgagg atgataaagt
    15421 ttccggacta cgtttaggtg ctgacgatta cctaacaaaa ccgttcagca taaacgaact
    15481 tatggctcgt gtcaattctc ttattcgtcg gtatactatg ttaaaccctg cttttataac
    15541 agacacagat tgcataataa taaatggcat gattattgac aagacaaacc gaattgtttc
    15601 tatcaataat attcctgttc aacttacaag caaagaattt gatttacttg cttttctggc
    15661 ttctaataag ggacgggtat ttaccaaaaa acagatttat acacaagtat gggaagaaga
    15721 atatgctttt gacgatagta acatcatgtc tttcatcagt aaattgagaa aaaagattga
    15781 accagacccc aaccatccat tttatatttt gacagttcgt ggtgtcggtt atcgttttaa
    15841 taaggaggct taacatgaat acaagtgtct atctactttt gatattggct cttagcctga
    15901 ttattatagc gtaccttgtt tctgtcctgc tacgtgtccg aatacaactt acacttataa
    15961 aagatgcttt agaggatata aaaaatggaa accttaatcg ccgtatttta acagatgaaa
    16021 acgacattac aaaacaaatt tgctatgata ttaacgaaat agcaatcaat agccaatttc
    16081 aacttattca gcaaaaacaa tcagaacaag cctacaaaca gctaatgaca agtctctccc
    16141 atgatgtaaa aactccactt gcttctttgg tcggttattt ggaagcagta gaaagtaaaa
    16201 ttgttgtagg agaggaaaaa gacgagtata tccatgttgc gtctaataag gctcattact
    16261 taaagaattt tgtagaaaat ctatttgaat gggtaaaatt ggattctaag gaacaagttt
    16321 ttcattttga tatttttgac ttgaatgaat tatcccgtaa tattatatct gattggatac
    16381 ctgttttaga aagcagtcat tttgaatatg aatttgatat accagaaata gagtacttct
    16441 tacgtataga cgcaaatgca tatacccgta tcattaataa cctattacaa aatattatta
    16501 cccatagtag tggcaacaaa atgacattga gaatttttga aaacaaagag caagctcaaa
    16561 ttataataac agataatggg aaaggtatat cttcagacaa tctcccacat atttttgaaa
    16621 ggttgtacca atgcgaccat tcacgagctt caaagggaaa tggtttaggc ttggcgattg
    16681 caaaggaact tattaacgct cataagggaa atattaccgc caacagcact cccggtatgg
    16741 gaacagaatt tacaatattg cttcctaaag ccctgtaaaa gtacgaggct ttcttactct
    16801 aaaacaagat aaaagcaaga ttatggcaag gttttggcaa ggttcatgtt ttataattct
    16861 aattgtagtt gattttgacg ttgtatcaac tatcttttgt agaaacaaat ttattaaaag
    16921 tgaggaaatt atatatgaat gattttgtga ttgaaacaaa gcaacttaca aaaatttatg
    16981 ggaatcaaac agtagttgac aaagtcaatc tccatgtaaa aaaaggccga atatatggtc
    17041 tgttaggacg caacggagct gggaaaacca caattatgaa aatgatttta ggattgactt
    17101 caatcacgta tggcgaggta gatgtatttg gagagaatat taagggatgt gaaaaacgag
    17161 tatatccccg tatcggtgca attatcgaaa cgccaggatt ttatcctaat ctgacaggaa
    17221 cggagaactt ggagattttt gcaaaacttc gtggtacagc cgcccctaat tctgtaaaaa
    17281 gagcattgga gattgtaggc ttaccttata aaaataaaaa gctgtttagt aaatattcac
    17341 ttggcatgaa acagcgactt ggtattgcca acgctatcct gcatgacccg gaactgttag
    17401 ttttagatga gccaaccaat ggacttgacc ctattggtat tgcggagatg agaaagttta
    17461 ttagggactt gagcgttgag catggaaaga caattcttat ctccagtcat attctttcag
    17521 aaatctcatt gttggctgat gatattggaa ttatcgacca cggtgttctt ttggaagaaa
    17581 gtagtatgag tgaactagaa aagaaaaaca gtaaatatat cctgctacaa gtttcagata
    17641 ttccaaagac atcacttatt ttagagcgtc aatttaacac gaaggattat tccgttcaag
    17701 atgaccacac tttacgcctt tataacacta ctctggatat gggtgaaatc aataaagcac
    17761 ttgtaatgca gaatgtaact gttattagtt ctcaactttg taatgatact ttagaggact
    17821 actttaaaaa aatcacagga ggagagggca ttgcttaaac ttattcaaac agaatttctt
    17881 aaactgcgtc gtaggaaact tatttggctg atgttgttgt ctgctctcat tatgccgttt
    17941 ttcgcactac tatacttcaa ttattttggt aaaacaggtg ttgagccaac aaagttttat
    18001 aaatggtctg catttggtta tacactttgg attattttgc ccgttgtttt gggtatattt
    18061 tgcaccatgc ttatacacga agaaaaccag tatgatatgc tcaaacagct ttggattgta
    18121 cctattagta aaatgggata cttttttagt aaatttttcg ttgttttgat ttactctatt
    18181 tgttttatgc tgattacagc cgttgcttct gtgttattta gtgtactttc tggatatgtc
    18241 gtatttagct gggcaagcat tttatatttg ttgaaaaaat gtttagaaat tggtgtaatc
    18301 actgcttttg taatgttgcc gattttggca atcgctacat ctcaaaaagg atacattctc
    18361 ccaatttgta tcacattggt atatgccttt tccggttttg ttataatgac aataaatatg
    18421 tatttacacc cattatccag tatgggagtt attatcatga gaaataaaga cattccaggt
    18481 cttatatttt cacaggaaat taatgtccca ttagcatttc tttgtatttg tatttggagt
    18541 attgtatcag tattgtttgc aagcattgca ctaggaagaa gaaagtgagg tgtagataat
    18601 gtggaaatta ctttgggctg aatgtcaaaa aattcgtcgt tcaaaaattg tttggattac
    18661 attttttgca tccattatga tagcagtcat agtttttgca ggagggcagg atgtatataa
    18721 tgacccagat ttacattatg gcttaaagag cgtttatgac ggttcacggt atatagacaa
    18781 tgcaggttgg tatatggacg cggtacaacc ttggtcaact ttttttgttc ttcctgctgt
    18841 cattgcccta ttaggaagct atattatttg tcgtgaagaa gaggaggaca ctattaaatc
    18901 cctgcgtctt attcccataa atgaaacaaa attaaccata gcaaaaatga ttattacttt
    18961 tatattcagt atacttcttt atttgttact ctttactata acatttttga ctgaatccgt
    19021 tttacatttt tccgatttat cgacaaaact ggttttaagt tgcatgaaag agtattttct
    19081 tgatggcata ggtgtattct ttgcaatttc tcccattatt gccttggtat cgcgcatgaa
    19141 aaaaggatat tggcttgcat tagtattcac agaagtttat tctattgctg gactatttgc
    19201 gggtatgtca aatgtcttac aaaccttttt tcctattgca gcaatattta atatttctgg
    19261 atactatata actactggag aaaaaattat ggggagtatc ataagtttat tactttgtgc
    19321 ctgcctttct gcatttatac tgaaagggct aaaacacaat gaaaaaaatt gaaataggta
    19381 tatcaaaatg aaaaagataa aaaaactgat tattctaatt gtattcttgt ttctttttgg
    19441 catgttacac tgttatactg aatggaaatt caacaatgac ccacctaaag gggtaataga
    19501 aagaataaat gagtggactg ataagattgg actggattag taacttatgt taattatagt
    19561 gaatgtaata ttagcaagtt attgtttcgt attttaatac tataacttta aatatttggt
    19621 tttaatcttc cgaacgaata taaaaaaact gtcacagagg caacatattt ttgaatattt
    19681 ataaagtggt agttttgatt ttcaaagctg ccgccttttc ttctatataa gcaaacttat
    19741 cctcaacaaa gtgtaaaaca tggtacattt taagaaaaaa gtaccacaga cacaagcaat
    19801 aaaagaaacg ccgagaacct acaaaaacgt agtgcttatg cagatttacg acgccttcta
    19861 ttttctcaac cgtcttaatg accttgtaca aaagaaattt ttctaatatt ttttataatc
    19921 ttatcttgtg atgtttttta tgtataaatg acttaataac aaatccaata aaaattatgg
    19981 atattggtat aattagaacg aggaatttat aatcattata atgtgacatt attcttaccc
    20041 aactctcaga aaaaatatat cctatactga ttaatgaagt attccatatg gttattccca
    20101 cgatagatga tattacaaaa gttataatat tttgacctgc tatacctgct ataaaagata
    20161 tgtatgttct acataaaggg attaatctac atacacaaac agaaatagaa gagtatttat
    20221 taaaataatc ttgagatgca aatattcctt tctttgcctt tggacatatc tctattattt
    20281 tatttatgat agatttacct ccccaatttc ccactgtata acaaaatatc gaaccaagta
    20341 tacctgcaat tatacttaaa attaaaattg tcagaaatcc aaaatgattt cttgcagcaa
    20401 ttgctccaca taaaggtaaa acaacttcgc taggtaatgg aaaacaagca tactcaatca
    20461 taattaatac aaatacagag attaatccat attgtaaaat aaaactattg attacagaca
    20521 cttacttatc tatctccttt cttatttaaa ggttttaagg gtacatattc taaggattct
    20581 caccctatat ttaaagaatc aatctcttta aactattata tgtatttaca caaagcattt
    20641 ataacatact ttaaaaatag taccttaaaa taagaaactt aataaataaa aaaa
//
LOCUS       DAELSY010000066        20619 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.066, whole genome shotgun sequence.
ACCESSION   DAELSY010000066 DAELSY010000000
VERSION     DAELSY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20619
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.066"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            78..1277
                     /locus_tag="KPG09_002104"
     CDS             78..1277
                     /locus_tag="KPG09_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBG0966714.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            1444..2229
                     /locus_tag="KPG09_002105"
     CDS             1444..2229
                     /locus_tag="KPG09_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0966715.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            2398..3963
                     /locus_tag="KPG09_002106"
     CDS             2398..3963
                     /locus_tag="KPG09_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0966716.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFLFTFLIKKFDMKTPGREEDNEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKVVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            3977..5308
                     /locus_tag="KPG09_002107"
     CDS             3977..5308
                     /locus_tag="KPG09_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG0966717.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARILGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMIEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            5322..5810
                     /locus_tag="KPG09_002108"
     CDS             5322..5810
                     /locus_tag="KPG09_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966718.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            5824..6291
                     /locus_tag="KPG09_002109"
     CDS             5824..6291
                     /locus_tag="KPG09_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0966719.1"
                     /translation="MFFKRKKEASTINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            6294..7448
                     /locus_tag="KPG09_002110"
     CDS             6294..7448
                     /locus_tag="KPG09_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0966720.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMILSLFGCLAYGLIVLLNYLQE"
     gene            7766..8518
                     /locus_tag="KPG09_002111"
     CDS             7766..8518
                     /locus_tag="KPG09_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0966721.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFIAIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPAVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            8519..10426
                     /locus_tag="KPG09_002112"
     CDS             8519..10426
                     /locus_tag="KPG09_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0966722.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQEENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSLIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKHIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEVKTKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMNDLSGIYILSLVICLIIFVIYGAVS
                     VNKFKSVVYKN"
     gene            10578..11252
                     /locus_tag="KPG09_002113"
     CDS             10578..11252
                     /locus_tag="KPG09_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0966723.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFMDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIDILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYII"
     gene            11252..12271
                     /locus_tag="KPG09_002114"
     CDS             11252..12271
                     /locus_tag="KPG09_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0966724.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDILKNRYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYINLYNCIENSGEIDSYLIDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDDYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSKIGSYTIFNIYFYDISDY
                     LSLDS"
     gene            12701..13861
                     /locus_tag="KPG09_002115"
     CDS             12701..13861
                     /locus_tag="KPG09_002115"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBG0966725.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAIIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDKNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGVHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            13863..14252
                     /locus_tag="KPG09_002116"
     CDS             13863..14252
                     /locus_tag="KPG09_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966726.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSNKNTKKEMEKNQRTDYNIPTISFK
                     SGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRGFSL
                     GFFRLYILFVLSFAIIGYFWIYKGIHI"
     gene            14513..14821
                     /locus_tag="KPG09_002117"
     CDS             14513..14821
                     /locus_tag="KPG09_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0966727.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEASVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            14910..16256
                     /locus_tag="KPG09_002118"
     CDS             14910..16256
                     /locus_tag="KPG09_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0966728.1"
                     /translation="MLDKFTEFLDSKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            16489..17586
                     /locus_tag="KPG09_002119"
     CDS             16489..17586
                     /locus_tag="KPG09_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG0966729.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDMGFT
                     GSEKALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFDFKPSTK"
     gene            17685..18599
                     /gene="murQ"
                     /locus_tag="KPG09_002120"
     CDS             17685..18599
                     /gene="murQ"
                     /locus_tag="KPG09_002120"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBG0966730.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNRVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            18709..19629
                     /locus_tag="KPG09_002121"
     CDS             18709..19629
                     /locus_tag="KPG09_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBG0966731.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDGIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            19646..20497
                     /locus_tag="KPG09_002122"
     CDS             19646..20497
                     /locus_tag="KPG09_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0966732.1"
                     /translation="MSCILKLKQIFEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
BASE COUNT         8110 a         2047 c         3617 g         6845 t
ORIGIN      
        1 cttttaactg tttaggtgat aataaaataa tgatacaatg tattaaaata taaaatctga
       61 aagtggatga gaattacatg aaaaagaaag tattattttt agtaattatt ttagcattta
      121 taatcttatt aggtaattct acttattctc aaaagaatca gttgtctgaa gaggaaaagg
      181 tggatgactt taattatgtg tataatgttg taaagacagg atatccatat ctaggtgtaa
      241 ataagagact taataatatt gattggttag caaataagga tgaatataca aaacgaataa
      301 aaaacactga aaatgatgaa gaatttatag aagaactgtc atctatatta tctgatttaa
      361 ataataaaca cacagaagta atagataata agaaaagata tgaacttttt aagaaatcat
      421 actctaataa tgattggtat gattttctta atgataaaaa agttgtggat agatatgact
      481 ctatgaatct taaaataaaa atgccagatg atatttttgt aaaaaaagaa ttaattttaa
      541 aggatgttat taagggtcag attggatata tgtatttacc atcaatgtca tcaaagaatg
      601 gctctataaa taatgattta aaaataattg gagattatat taacacttta gaaaaacata
      661 aagctttaat tattgatata agaggtaatt taggtggaag tgataggtat tggcagggaa
      721 tcgtatctaa actcataaaa aatgatgtca aaataaatgg ttatagaatt tatagaaata
      781 ataatgaaat agtaaaaaaa tatacaaatg caagaaatat aaaattaaat cctgtagaaa
      841 atttaccagc aagtgtaaag gaaaatgcac ctaaagaaat tataaaagat tttaatgatt
      901 ttgaagatac ttcctatact ataaaatcta cagatgattc aaaatttaag ggtaatatat
      961 acttacttgt tgatagaaaa atatattcat catcagagtc atttgcaatg ttttgtaaag
     1021 aaactaaatt tgcgacttta gttggtgaaa ctactggcgg agatggaggg tgtatagacc
     1081 ctgtattgtt taatttaaaa aatagtggtc taatagttag aatggcaagt tgtatgtatt
     1141 taaataagaa tggaatatgt gatgaggaat ttaaaactat tccagaattt aaagtaaaag
     1201 attgtaaaag gactgctgat tttaaagatg ataattgcat aagaaaagca ctagaacttg
     1261 aaaatattaa ttattagata aaagaataaa attaaaacta cattttttta aacatcaatt
     1321 atagcagtat tttttatagt caattttatg ggctaaatag taaatataag aatacgctgt
     1381 aatttaatgt tgaatattgg tataatattt atgacgattt ttaattaaag gggataatag
     1441 tttatgagat tagaagaaat gattttttta aattataata aattgaatga tacagattta
     1501 ttaatatgga aatatataac tgcaaataaa aaacattgta gtaagatgtc aataaatgaa
     1561 cttgctatga aatgtaatgt gtctcgtgca actattacaa gatttgttaa aaaattagat
     1621 ttaaaaggtt tcagtgagtt taaagtatta ttaagctggg aatcagaaaa ggttcatgtc
     1681 atagaaaatc agtcatttga tattgcatgt aattcaataa ttaagtatgt tgaagatcaa
     1741 aaaaataaag attatgatgc attgtgtaaa cttctttatg aatcaagaac aatatacgct
     1801 tatggttcag gtgatattca aaatgtggtt gcaaaacaga taaagagaat gttcttatca
     1861 tgtcaagaaa taatatatga ttttgaggga agaacatttg atgattcttt ttacaatatt
     1921 gtcagtaaaa atgatgttat gattattata tccctaagtg gtgagaatga gaatacactt
     1981 aatattgcta gaagattaaa attaatagga actaaaataa tttcaattac agagtttaaa
     2041 aataatacat tgactgattt aagtgatgaa tcaatttata tttcagcgac taatatctca
     2101 tttttacaaa tgcaccctag ttataaattg acaatgttat attttatatt gattgagtta
     2161 ttatttatta aatatagtat ttataaaagg catagaatga ttacagaagg gattgattgc
     2221 aaattgtagt cattcttttt tttatgagaa tttgaacata aaaatgtgca aaaacttatt
     2281 tgactgtgaa taagctccaa atgtacccta aagattgtat atatttatta acctgttaaa
     2341 ataatagata tctaaggcca aagataaact aaaaatatga ggaggggagt aatttatatg
     2401 atgaagaaaa tacagcgttt tggaggggca atgtttacac caacactatt atttgctttt
     2461 gccgggataa tggttggatt ctcaattgta tttcaaaatc agtcaattat gggttcactt
     2521 gctacaccag aaaatatttg gtaccaattt tggggtgtaa tatctagtgg agcttggatg
     2581 gtatttaatc aattaccatt attatttgct atttctttac caatagcact tgcaaaaaag
     2641 caacaagcaa gagcttgtat ggaggcttta gcaacgtatt tgacttttaa ttattttgta
     2701 ggttctatgt taagtttttg gggaaaaagc tttggagttg atttcgcagc tgaaataagt
     2761 gctggaagtg gacttgtaag cattgctggt attaaaacat tagatactgg tatggttggt
     2821 gctttgttaa tatcaggtat tgtcatttat attcataata gattttatga taaagaacta
     2881 ccagatttta ttggattatt tagaggctca tcacttgtag ttgcaatttg tttctttgtt
     2941 atgataccag ttgcattatt aacttgtttt atttggcctc atattcaaaa tgttattaga
     3001 catttgcaaa cgttttttat aaatagtgga aatataggag tttggtgtta tgcattctta
     3061 caaaagattt taataccaac tggattacat cattttgtat atgcaccaat ttgttatgat
     3121 agtgttgttg ttccaggtgg tacatcagta tattgggcta ctcatattca ggactttcaa
     3181 acttcagcta agactctaaa ggaaatgtat ccaatcggat tttcattatc tggattatcg
     3241 aaggtgtttg gttcattagg tgtatttggt gctttctatg taactgcaaa gccagaaaaa
     3301 aagaaaaaag tattaggttt aatgattcct gctactctta ctgcagtatt aactggaatt
     3361 actgagccgt tagaatttac atttttattt gtagcaccac ttttattcct agtacatgca
     3421 tttttagatg catgcttgca aacgatttcg tttgccttag gagttgttgg tgattttggt
     3481 ggaggaatta ttaactgggt tgtattaaat tggcttcctc ttggaatgta tcactggaag
     3541 gtatatgttg ttcaagttgt tgtagggatt atattttcat ttatctggtt ctttttattt
     3601 actttcttaa ttaagaagtt tgatatgaaa actcctggta gagaggaaga taatgaagaa
     3661 acaaaactat atactaaaaa tgaatactta gaaactaaag atgaaaaagg aaataaattg
     3721 agtaaagcct ctcaacaagc atctgaatac ataaaattag ttggtggagc agaaaatgtt
     3781 gtagatgtta caaactgtgc tacgaggttg agattaacct tgaaggatga ctcaattatt
     3841 tctaaagaag aagattttaa ggtagtaggt gcacatggtt tagtgcataa tggaaaagct
     3901 attcagatta ttattgggct ttcagtacca agtgtaagag aagaatttga aaatttatta
     3961 taaaggagaa ggaaagatgg aaagaaaaag acagaggata ctaattgctg gtgggggtag
     4021 tacttatact gctggaattg taacaatgtt aattgaaagt gttgctaaat ttcctattga
     4081 atcaattaaa ttatatgata ataatgatga gagacaaaga aaagttgctg aagcatgtgc
     4141 aataattgtt agagagaaaa atccagaaat taaatttagt tatacaacaa atccagaaga
     4201 agcatttact gatattgatt ttgtaatggc acaaattcgt gttggtttat atgctttacg
     4261 tgaacaagat gaaaaaattc cattaaaata tggggttgtt ggtcaagaaa cttgtggggc
     4321 aggtggaatt gcctatggat taagaacaat aggtccaata atcgaaatga ttgattatat
     4381 ggaaaaatat tctccaaatg cttggatgct taattattca aatccagcag ctatagttgc
     4441 agaagcatgt cgtgtattaa gaccaaattc aagaattatt aatatttgtg atatgccagt
     4501 atgtttagag gaaatatttg ctagaatttt aggtttaaat tctcgtaaag actttgatgt
     4561 tagatattat ggattgaatc atttcgggtg gtggacaagt attaaggaca aagaaggaaa
     4621 tgatttaatg cctaaattac aggaatattg tgctaaaaaa ggatatgagg aatttacacc
     4681 tcaaggtcaa cacaaagaat ctagttggtt agaaactatg agagcagcaa aggacttatt
     4741 agaaatagaa ccgactacat tacctaatac ttatttaaaa tattatttaa tggctgatga
     4801 aactgttgaa catgctaatc caaattatac aagagcaaat gaaattatgg atagaagaga
     4861 aaaggataca ttcgaagaat gtgaaagaat tataaaaaat ggcacagcaa gggatacatg
     4921 gtttgatgca agtgaacatt ctcaatttat tgttgaatta gcttgtgcat tagcatttaa
     4981 tactcaggaa agatttttac tgattgttcc taataatgga gctattgaaa attttgcaga
     5041 tgatgcaatg attgaaattc cttgccttgt tggaaaagat atggttgaac ctatgtctat
     5101 aggtaaaatt ccaactttcc aaaaaggttt aatggaacaa caagttgctt ctgaaaaatt
     5161 agctgtagaa gcatggatag aaaaaagtta tcaaaaatta tggcagtcgt ttactatgag
     5221 taaaactgtt ccaagtgcaa aagtagcaaa agaaatctta gatgaaatga ttgtggctaa
     5281 taaggatttc tggccaactt ttaaataagg agaacaaaaa catgataaca aataaaataa
     5341 aaaatataac agcagtatta aaagatgaaa atatatttct ttcagtggtt atggtttgtt
     5401 ttctaatatt acctgtttta tatatagtgt atttagaatt gacatcatta tttacaaaca
     5461 tttcattttc acaactttta aacaatgatg ttaatgcaag tttaaattta atctctaact
     5521 ttagtgcagt atacagtgca tatgttattt acaatataaa aaagaataaa gagataaaag
     5581 aaaacatgat tgctttctat ttagtcttat tagggcagat attccaaatt tacacaataa
     5641 ctattgttat catgtttacc tatatttata aatttgttgg aattaaacaa atcataagca
     5701 gtattaaaga actacaatgg ggaaagagct ataaggctct tttttcctca attgcagtat
     5761 gcttaatatc gttatttgta atgataatta gaataaagtt aattatgtag aggagtgtct
     5821 ataatgttct ttaaaagaaa aaaagaagca agtacaatta attcatacat tgatggaaaa
     5881 ataattaatc ttggagaaat tcctgataaa gtattttcaa ctaaggtaat gggagatgga
     5941 tttgctatga tagcaaagga caatattctt aaatcaccga ctaaaggagt aataagtttt
     6001 attgctcaaa ctaaccatgc tataggaatt aaattgcaaa atgatgttca aattcttatt
     6061 catattggat tggatagtgc aacaacaaaa aaagaaaact ttaaattaga agttaaacaa
     6121 ggtcaagaag ttaatatggg ggaaaagtta gtagaaattg ataataagtt acttgaaaat
     6181 actttatata ttcctttagt tattgtagag aatcctaata cggtaaaata tcatctttca
     6241 aacttaaatc aggaaggaaa tagtggtatt aaagttatca attttgattg attatgatta
     6301 ataaagcgat taaatttaaa tttatcatat tctatttttt ctcatatatt atttttgcct
     6361 ttgcttcaac aaaattcaca cctttcttat caaaattagg ttactcagcg tttgaaagag
     6421 gaattatatt atcgagctat gcaataacca atatattatt tcaattatta tttggagtat
     6481 tagctgataa gtatcaaaca atgaaaaaaa ttgttttaat tagtttatca gtttatggaa
     6541 ttgcatcagt tattatgttt tctactgaat ctgcaacttt tataatatac ttattacttg
     6601 ttgctttatc aggaggatta ttaaatactt gctgtagttt atatgataca tggataatag
     6661 gttgtggaga taatattaat aattatttgt catttattaa gacttttggg tcgattggat
     6721 gggctattgg aagtatggta gcatcttact tgattcttag attctcatat tttggattat
     6781 ctgtagcgat tggtttaatt gtagtattac ttattataaa tatactatta ttacctgata
     6841 ttgaaaagat taataagaaa ataaatgtaa ctaataaaga tattattgaa ttattaaaag
     6901 ataaaaaata tatcttacta gtatgtattt tattcttatt atactcaatg gtggtagcaa
     6961 ataactgtac tgtaatagat aaaatgatag ctttaaatgc tacaaattct cagatatctt
     7021 taaagtggtc tttacagtca ttattagaga taccaacata tctggctggt tcttatttat
     7081 taaaaaaata tagcagttta aacttattaa aattaagtgc tattatgata acagttcagt
     7141 ttctattatt tgctttaaca aatgattcaa atatcattat tatactgtgt gtttttcaag
     7201 tgttttctac tcctttaatt ttaattactt caaaaagatt aatatttgaa gtttctccta
     7261 aaaaacttag aagtagtagc caattaatag ctttaagtat atttatggga ggttcatctc
     7321 tgattattcc aactgttgct ggttttttga gtattaatat tgggtataat tatacattaa
     7381 tgatattaag tctttttgga tgcttagctt atggcttgat agtcttattg aattatcttc
     7441 aagaataatt gttttaatat atagtagtaa tatcacttgt ttatagtacc tgtatttgcc
     7501 acaatcaagg ttgtattttt ggttttttat agagaagaat tattgtatag attagaggct
     7561 tcttaggggc ttcttttctt tgtttataca aaaaagtgaa tcatgctttt attttaagat
     7621 atttccacta taattataat agtcttccat atgttacaaa agtgtaatgc taacagttaa
     7681 aatgtaatat ttacttaagg atttatttat ataaaacctt taaaataaat atatattaaa
     7741 aaattagcaa ctggaggcta ttattatgaa agaaatatta aaaataaaaa atatatcaaa
     7801 agattatgga ataaaaggat ttaaaacaaa tgtgttaaaa aatatatctc ttacagtcaa
     7861 tgaaggtgat tttatagcaa taatgggtcc atctggtgca ggaaagacaa cattgctaaa
     7921 tttaatgtct acacttgaca aacaaacttc aggagaaata atactagatg gaattaatat
     7981 atcaaaagta aaaaataatg aactatcaaa attaagaaga gaaaaaatag ggtttatatt
     8041 tcaagactat aatctattag acaatatgaa actaatggac aatatagcac ttcctctagc
     8101 attaggaaaa aagaaaagca aggagataga agctaaggta ttttctatag ctaaaaaatt
     8161 tggattagaa aatcatttag ataaatatcc atatcagtta tcaggaggac aaaaacaaag
     8221 aggcgcagca gctaggtcat taattacaaa tccagcagtc atatttgctg atgaaccaac
     8281 aggtgctttg gactctaaat cagcatatga acttttagaa tcattagaaa aaataaacag
     8341 agaaaacaac gctactatta taatgattac acatgaccct ctaacggcaa gttattcaaa
     8401 tgaagtatat atgataaatg atggaaatat aaagtgtaaa ttaaacaaag gaaatagtag
     8461 aaaagaattt tatggaaaaa ttatggatat gctagcctct atgggaggtg agatgtaagt
     8521 gagtatactt ggaattgctt ataataattt taagagtaac ataagaactt atctagcatt
     8581 ttttatatct atggtttttt cagtagttgt gctgactaat tttgaattat taagatatgg
     8641 agatgctata aatgtgttac aagaggaaaa taaaaaattt acactatcag tgttagtagc
     8701 tgtaataatg atactatcag tatttttatt tttctttata tggtatgcaa caaatatatt
     8761 ttttaagaat aggtctaaag aaattggaat attgtccttt atgggattag atttatatac
     8821 tataggaaaa atatattttg tagaaaatat gcttatagga ataagttctt gtataacagg
     8881 aataattata gggataataa catctagatt ttttcaagta gtgataataa aattatctgg
     8941 atttaatata aaggtatcta atggacttag catagaagct atactatatg ctactttaat
     9001 ctttatttct atatttataa taatggctgt aaaaggtttt ataacaatat gtagaagtag
     9061 tgtaataaat ctaatcaata tatctaaaaa acaagaaaaa ataccaaagg taggaatagg
     9121 tatatatata ttagctatag tatcacttat agtaatagga tatggatact atctttctac
     9181 gaatataaga actggagata taactagtgt actacctgtt ataggtataa taatagtagg
     9241 tacttatggc ttattcaaat cagttatgcc tgttatattt gatattataa tcaaaaataa
     9301 aaaaatatta tttaatggaa ataatataat tgcgataaac aatataaact accgattaaa
     9361 taaaaactat aaaacttatg ctataatagc tataattata actacaacaa tatcaactct
     9421 tggtgcttca gttgctatga agcatataca taagaatgcc caagaacaaa gaaatgtgta
     9481 cactattagc atagtttcaa tggataaaga tgaagtgaat aaaaaacaaa tagagaacat
     9541 aataaaagat actaataaga taaagtactc cgtaaatcca gaactaacaa tcttaaagaa
     9601 aacagatata aaagaagctt atgatacaac agatactata atgaaatata gtgatttttg
     9661 taatactctt aaagtgaatg gcaatgataa agaattaaaa gagtatggtg aaagattagt
     9721 tagtggaaat aatgtaatag agctaaaaag tcctcaaacg atagcatcaa tagctacaaa
     9781 agaagtcaag acaaaacttg atggtcagtt atataatatt tcaaggggag aagtaaaagt
     9841 tccagtatta gggacaggac ttaatcaaag tattatagtg gtaaatgatg atacatattt
     9901 taaactaaga gataagggtc aacaagtata tttttatggt gcaaaggtat ttcaagaaga
     9961 aaatagccaa gtaatgttca acaatattga aaagaattta agtttaaaaa atggatatat
    10021 tcagaatgga tataaagctc aaggtgagag tcaatggatg aagtttgcat atgtagtgct
    10081 tgtgttttta ttcttagtct ttgcaattgt agctggaagt ataatatata tgaaaatata
    10141 tagtgatgca tatgaagata aagagaaata caagatttta ttaaaaatag gtactacaga
    10201 aaaagatata aatagagcta tacttaaaga agtagctata ttttatactt tgccaatgtt
    10261 aagtgcaact atatctagtt attttgcatt gagattagca ggagatttat tgatgaacga
    10321 tttatctggt atatatatac taagtttagt gatatgtttg ataatattcg taatatatgg
    10381 agcagtatct gttaataagt ttaagagtgt agtgtataaa aattagttga tatattaata
    10441 aagagaaagg agattcttgt aataagagtc tccttgttga ttaactaaat aatttgggta
    10501 ataaatttat ataaaatatg ttatgatatt aataattcat ggcatagtga aatattagga
    10561 taatggagaa tatacaaatg tatagaattt tattagtaga ggacgatatt gatttatcaa
    10621 aagagattgc gttagcactt gaaaaatggg gatttaaagt agaattaatt gatgattttg
    10681 aggtagtatt agatgaattt atggataaaa aaccagatgt agtactttta gatgtaaatt
    10741 taccattata taatggattt tactggtgcg aaaaaataag agctatttca aatgtaccat
    10801 taatattttt atcatcaaga gattctgata tggatttaat aatgggtata aataatggag
    10861 cagatgatta tattaccaaa ccattttcta tagacatatt ggttaccaaa ataaatggta
    10921 taataagaag agtctataat tatagtgatt ctaatagtat tttgtattgt gaagatttga
    10981 tgtttgatgt ggggaaaggg attataaaac ataagtataa ggataaaagt atagagttga
    11041 ctaaaaacga aataaaaatt ttaacattat tattaaaaaa taagaatagg gttgtatcaa
    11101 gggaaagtct tatgatgact ctatgggata atgatgagtt tgtgacagac aatgcactta
    11161 ctgtaaatat gaatagattg agaagtaaag ttaaagaact aggttttgat gattttataa
    11221 aaactaagaa ggggatagga tatataatat aatgctagta aaatatttaa aaggtaggtc
    11281 taagtatatt gctctacctt tagttgtagt tagtataata aatatctatt tatttgctat
    11341 agatatttta aaaaatagat attcagaatt aatatactta gattttttag tgctgtttat
    11401 aataatggta ttttttgtta tagattatat aaattttaga aatagttata taaatttata
    11461 taattgtata gaaaatagtg gtgaaataga cagttattta attgatggac aatcttttga
    11521 agaaaatcta ataaaagata ttatagaaaa cttaaaaata aaaaataata gtgacataga
    11581 gacttataaa caatcattaa aagaattaga tgattacata gctaaatggg ttcatgaaat
    11641 aaaaattcca atatcttctt taagtataat tacagatagg ttaagtagca tagaagatag
    11701 tttggatatt aagaatcagg ttgcaaagat aaatttttta gtaaactcta ttttgtatag
    11761 tagtagaagc aacactatgt ttgaagatgt atttataaat aaatttaatt tagaaaaatt
    11821 ggtaaaaatg tctataaaaa ataattcatt tttgcttata aaaaataatg tagaagttag
    11881 tttgaatgaa ttagaaaatg atgtttacac agattctaaa tgcatgtcat atgttttaga
    11941 ccaaattatt aataatgcta taaagtattc aaaggaagtt ggtaagattg aatttaattc
    12001 aaaaaagtta gaaaatggag tggtattatc tataaaagat tttggtatag gtataaatga
    12061 ggaagatata tctagggttt ttgacaaggg atttacgggg aaaaatggaa gaaatcaatt
    12121 gtataagtct actggaatgg ggatgtattt tgtaaagaaa atgatagata gtttagggca
    12181 tgagattgag gtatgttcga aaattggttc atatacaatt tttaatatct acttttatga
    12241 tatatcggat tatttaagcc tagatagtta ggttattaga ctataaaatc tatcatcata
    12301 tacaaatcga catatacaat aagaataaat ttagttcttg aaaatagtga agatttaatt
    12361 agtgccttaa atgatgttct atctatatta taaaataata attattatat atgaaaaaca
    12421 tatcagatag gtacctggta tgttttttat ttgtagtaac tattttaata tggtttttgg
    12481 agagagaata tgttttgttt gtgaaaaaaa attacatgca ttacattgac actgaaaata
    12541 aattaatgta agatttagct aaggtaaaaa acgtgtataa gattaactcg attaatttcg
    12601 taagagtaaa ttatcgcaat taaagatgaa ataagattaa ttctaaaatt attaaactta
    12661 ataccttgat ttggggagga ataactataa acttaaaagg atgataacta tgtatagtgt
    12721 aggtttaatg tcaggaactt ccttagatgg agttgatgca gtactagctg aaattagtgg
    12781 gaatggtaga aatactaaag ttaagcaaat agaatttata actctagaaa ttcctaaaga
    12841 tataaaagac gaaataagaa agtgctgtat agaagaggaa tcgagtgttg atttaatctg
    12901 tagcttaaat tttaagcttg gatatttatt tagtaaagct gtaaaatcag tgtgtcataa
    12961 agcaaacttt catatagcaa atttggattt tatcgcatct catggtcaaa caatctttca
    13021 tataccaaga agttataata attttgtatc ttcaacgctt caaataggtg aaccagcaat
    13081 tatagcatat gaaacaaata caaaggtaat atctaatttt agagtaatgg atatagcagc
    13141 aggtggagaa ggtgctccat tagtaccata tagtgaattt ttattatata gtgataaaaa
    13201 taaaaattta gcacttcaaa atattggtgg aataggaaat attactataa tacctaaatc
    13261 atgtaacata gatgatgtat ttgcatttga tacaggacca ggaaatatga taatagatgg
    13321 agtatgtcaa agattattta atagaaagta tgataaaaat ggatactttg catcaaaagg
    13381 taaaattaat gaagaaatgt taaaagattt gatgagtcat aagtatctta gtcaagcgcc
    13441 acctaaaagc actggaagag aggtatttgg acaagtctat ttggataaca tgcttaacaa
    13501 atataaacat gtagataaat atgatttgat agcaacagta actatgttta cggcaaaagc
    13561 tatatattat aattataaaa acttcatact tccaaaggtt aatgtagata ctttattaat
    13621 tggaggtgga ggagttcata atctaacttt aataggatat ataaaagagt tattattaga
    13681 ggttgaagta ttaactcaag atgaatatgg gtattcttct gatgcaaagg aggctttggc
    13741 atttgtaatc ttgggcaatg aaacattaaa taattctttt tctaatgtaa tatctgcaac
    13801 aggggctaaa aataaagtta ttttagggaa tataacacct aaaccttttg gaggtaaatg
    13861 acatgtctaa taataaaaaa aagaaaagaa agaaaaataa taattataaa aatgcaaaaa
    13921 gtaataaaaa tactaagaaa gaaatggaga aaaatcaaag aacagattat aatattccaa
    13981 ctataagttt taaaagtgga ttaatactaa tagtttcatc tctagtaagt actgtagttt
    14041 ttcctttgtt attatctata tttggaatag ataatagatt tggagttgtt ataggaaatt
    14101 cattaattac atcttttgca gttgcatata caagatactt tgttgaaact aagagaggtt
    14161 tttctttggg attttttaga ctgtatattt tatttgtttt atcatttgca ataataggtt
    14221 acttctggat atataaagga atacatattt aaaaaagtat tgagactatt ttatgttaat
    14281 aaaagatagt cttttttctt ttttaagaaa agatatgagt attttaagta gtgtgtaatt
    14341 ttagacaaaa gaatgattag ataaataaag caattaaagc aagatataaa tgcatgaaat
    14401 aaaattacaa ttaaaaatgt aaaatatgaa aaaatatata caaacgtatt cctgtaatca
    14461 aaaatacatg ctataatgat tgaaaagaaa taaaaaaaca gggggaatca atatgagagt
    14521 attatttgtt tgttcatcag ggatgtcaag tgctatagca gttaatgcat tacaaaaaga
    14581 aggagcaaaa aatggaatag atatagatgt tttggctgtt ggtactcaag agtttgagaa
    14641 tgaagttaaa aatggatggg atattgctat ggtagctcca caagtcagac atagatttga
    14701 ctatttaaaa gcttttgctg atgaggctag tgtaccatgt gcattaatac aagcacaagc
    14761 ttatagtcca ttaggaggac caaagttatt aaaacaagtt caagagttgt tgagtaaata
    14821 gtagttgcag tgttgttagg ttagaagaaa gttaatttta taaaaatttt aatgataata
    14881 aaaataaata ttaattaagg agagaaaaga tgctagataa atttacagaa tttttagaca
    14941 gtaaattatc aacttctatg gccagattgg cagaacaacg tcacttaaga gctgtaagag
    15001 atggaatcgt agcttcatta ccacttataa tagttgggtc atttttcttg atattagcat
    15061 ttcctccatt accagattca tggggggtaa ctcagtatct aaaggaaaat gcagttacaa
    15121 tattactacc atatagaatg acatttttta ttatatcagt atattctaca tttggcatag
    15181 gttatagttt ggcaaagtca tataagttag atggattgac aggaggttta ttagctacag
    15241 ctacattcct tttgacaata gttcctactg caatagaggg tcttacccct gatttattaa
    15301 agatagttga aaagagtcca gaacttttag cttggtataa ttcaattcct caaggatggc
    15361 aaatgccaat ggctaatctt ggtggaggtt caatgtttgt tgctattgta acatctataa
    15421 taggtgttga gatttataga tttactgaca aatctaaatt caagataacc atgccagaag
    15481 cagttccagc atcagtcaca aggtcttttg aagctctgac accagccgca ttaataataa
    15541 ttttaatggc tattgttact tatttcctag ggtttgattg gcatggcttc atagctaaat
    15601 tagtaactcc attagtttca gccactgaca cattaccatc agtaattata ctaatatttt
    15661 taataacgtt tttttggtca tttggaatac atggtgtatc aataataggt tcattggcaa
    15721 gacctttatg gatacaatta ttagatgcta atacagcttc atatgctgcg ggtcaagcag
    15781 ttccaggtat tgctgctgaa cccttttttc aatggtttat atggattgga ggttctggag
    15841 ctacaatagg cttagcttta cttcttgcat ttagagcaaa atcagcttat ggaaagtctt
    15901 taggaaaagc aactgtaata ccagcaatat tcaatataaa tgaaccaata atatttggag
    15961 caccaatagt attaaaccca atattaataa taccatttgt tgtggcacca atagtaatgg
    16021 caacagtttc atggtttgct atgagtgcta atttagtatc cagagtagta gttactgctc
    16081 cttggacatt accaggacca gtaggggcat atttagccac tggtggagat tggagagctt
    16141 cagttcttaa cttattatgt atagctatat caataataat ttactatcca tttgtcaaga
    16201 tgtacgataa gagattgctt catgaagaaa aagttgaaga gcaagaagct tattaattag
    16261 aaactatatt ggaaattaaa aactatatta gattagtata aatttcaaag tataacagtt
    16321 atgaaataag taacgaagat attttgttga ttgtttataa aaaaagaaaa ggtaaacttt
    16381 tcttttttta taaatatata tgaaactttt tttcaccaaa tttttaaaaa agtttcgtgt
    16441 aattcgaaat aaatttaaac acatatagga tataaggaga tgacacagat gggaagatta
    16501 ggaatatcaa tttatccaga aaaaacaaca tctaaggaaa tatatgatta tatagataaa
    16561 gctgctgaaa atggatttac acgtatattt tcatgtttat tatcagttaa agatactaaa
    16621 gaaaatataa ttaaaaaatt taaaagtata aatgaatacg ctaagtttaa aggttttgag
    16681 gtaatacttg atgtaaatcc cagagtattt ggtgaattag aaataagcta caaagatttg
    16741 acttttttta aaaagacgaa ggcagacgga ataaggcttg atatgggatt tacaggttct
    16801 gaaaaggcct taatgacttt taatcctcaa aatcttaaaa tagaaatcaa tatgagtaat
    16861 aatactaatt atatagatac aataatggac taccaaccaa ataaagaaaa tttgacagga
    16921 tgtcataact tttatccaca tagatatagt ggattaaatt tagaacactt tataaaatgt
    16981 acagaaagat ttaataagta tggattaaga acagcagttt ttgtaacaag tcaaaatgaa
    17041 accacatttg gaccatggcc tgtaactgat gggcttccaa caattgagat gcacagaaat
    17101 ctacctatag atattcaagt aaagcatata atagctatga ataatataaa tgatataata
    17161 atatcaaatt gttatccaac atatgaggag ctaaaggtat taggttctat gagaaaagat
    17221 atggttacat ttgatgtagc aatagaggaa aatatacgag aagttgagaa gaagatactc
    17281 tttgaagaaa tacattttaa tagaggggat atatctgaca gtctgataag gtctactcaa
    17341 tctcgtgtta aatataaagg atacaaattt gaactattta atactcctaa aataataaaa
    17401 agaggagata tagtaattga aagtagtgaa tatggtcatt atgcagggga gttacaaata
    17461 gccttgactg atatgaaaaa cagtggtaaa tcgaatgtag taggatatat aagaaaagaa
    17521 gaattattta tattagatta tattaaacca tggcaaaagt ttgattttaa accctcaaca
    17581 aaataattta ttatgaaaat attaatttat tataaaaata taattatgaa agttggagtt
    17641 aaaatgtgtt taaaaattta atattaagaa aatgaggaaa tgatatgtta ataaaaactt
    17701 tagaaaattt agttacagaa ggtagaaata agaatacatt gcaaatagat aaagaagata
    17761 cattaggcat aatagaatta ataaataacg aagataaaac tgtagcctat gctgttgaag
    17821 aacaaaagga gagtatagca aaagctgtta atataatagt agatagaatg aagcagggag
    17881 gtagattatt ctatatagga gcaggtacca gtgggaggat aggaatacta gatgccacag
    17941 aatgtcctcc aacttacgga gtggattttg agcttgttca agctataata gctggtggaa
    18001 atcaagcaat atttaaagca atagaaggtg ctgaagatga taaagaactt ggaaagcaag
    18061 atataataga tagaggagtt acatctaagg atgttatatg tggtatagct gcatcaggaa
    18121 ggactcctta tgttataggt gcaatggagt atgctaagga attggggtgt gcagtactat
    18181 caataactat gaatccgaat agtgaaatga gtaaaaaagc ggatttacca ataaacatta
    18241 tagtaggggc agaagtaata atgggttcaa ctagaatgaa aagtggtaca gctcaaaaaa
    18301 tggtatgtaa tatgttaaca acagcttcta tggttaagat gggaaaagta tactcaaact
    18361 tgatggtaga tgttaagaca tcaaatgaaa agttggttga aagagcaaaa agaataataa
    18421 tgatagctac aaatgtaaaa tatgacgttg cagaaaaatt cttagaagaa gcagataata
    18481 gagttaaatt ggctatattt atgataaaaa gtggtttaga taaggatagt gcaaagagta
    18541 tcctagatag acaagaaggt tatataagtg aagctttaaa atcaatagaa aaattgtaaa
    18601 ataaataata gtactgataa cctttcaata ttaaaaaaac agcattggaa ggttattttt
    18661 agatattaag tattgaaaga attattaaag atattgtagg aggatagtat gtatttttta
    18721 ggtgtagatg gtggaggaac gaaaacaaca tttacattag tagatgaaga attaaatata
    18781 gtaggaacca taaccaaagg aacttgtcat tataatcaaa taggatttga taatctaacg
    18841 aaattattaa taactgggtt ggaagaagtt tgtaaagatg ctaagataaa tgtagaagaa
    18901 ataacgtatg catttgttgg acttgctgga tatggaaaaa taaaagaagt attgtatgca
    18961 ttagaagtag caacaaaaaa tgcatatagt catataaatt atactctagg taatgatgta
    19021 gaaatagcat tagcaggttc tttaaatggg gaaaaaggta taaatataat tgcaggtact
    19081 ggttcaatag cacaggcttt agataaagat gggaatcttc acagatgtgg aggatggggc
    19141 tatgtattag gagatgaagg ttcagcgtat tatataggaa tggctacctt aaaaatgttc
    19201 acaatgcagt ctgatggaag gtgttcaaag acaaaattat atgatttaat aaaaagacat
    19261 cttaatatag agaatgatta tgacataata aaatatgtaa atgatggaat acaaggagat
    19321 agaatagaaa tagcaaagtt tgctacgata tgtttaaaag cagttattga aggtgataat
    19381 actgctagta aaatatttga tgatgcagct tatgaattaa gtaggcttat aataggatta
    19441 gagcatcatt ttgaacaagg tacaaagata aaggtatctt attcaggtgg agtgtttaag
    19501 tcaggagatt taatattaga gccacttaaa aaatatttga ataaaacgag atttggtata
    19561 gtgagaccag tgttgactcc agatttagga gcatgtttac ttgctaagaa gaaatattat
    19621 agtaaatagg tagaggtggt agagtatgag ttgcatttta aaattaaaac agatatttga
    19681 agatttaacg gaagtagata aaaaaatagc tgattacata cttaataata cagaggctat
    19741 aagtaagctt tctgtatcag aattggcgtc aaactcaaaa acaagtacag caagtatagt
    19801 taggttttct agaaaaatgg gatattcggg ttttggggat ttaaaaattg agatagctaa
    19861 agatttgatg ggcaaagaaa atgaatacac atatgtagat gaaaactatg attgtaatat
    19921 agatagtgtt ataaacaaaa ttacaaacaa aaacatagag actatcaacc aaacaaggtt
    19981 gttgaatgaa aaagatataa taaaagaagc agttgaacag ataataaagg ctaaaaatgt
    20041 atatatattt ggagtaggtg gttcggcttt agttgcacta gatttacaga tgaaactttt
    20101 aaggataaat aaacaggcat ttacctcttt agatagtcat acacaactta tggtatcttc
    20161 aaatgtagat aaagaagata tagctatagc aatatcttac tctggagaat caaaagaagt
    20221 aataaagtct atagaaaatg caaaattaaa aggatgtaag gtcatttgta taactaaata
    20281 tagtgacaat catcttagta agattagtga tttaaagcta gtagtaccaa atatagaaaa
    20341 aaggcttaga gaaggagcta tttcttcaag aatagcaatg cttacgttga tagatataat
    20401 atatatttct ataattcaag aaaacttaaa tgaggcagaa cagaaactac aagaaacaaa
    20461 aaggatactt gattatttaa atattaataa tgcttaaaac taggttatat atcaactaaa
    20521 caaataagtt agactataag cgtaagtcaa taagaaatag ataaatatct aaaatattga
    20581 cttacttaat aataatatat agcattttta taatacaaa
//
LOCUS       DAELSY010000067        20419 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.067, whole genome shotgun sequence.
ACCESSION   DAELSY010000067 DAELSY010000000
VERSION     DAELSY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20419
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.067"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(245..5302)
                     /locus_tag="KPG09_002123"
     CDS             complement(245..5302)
                     /locus_tag="KPG09_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBG0966733.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVTSALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNSDRQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTSEDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKFKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGIDYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDESGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQLVQPFKQVFREYYRINSDEISEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNINFEGSHV
                     LITGNLGEYSVHMGSGIVHKKGMGALNILPVHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDNKIKDPSILSNL"
     gene            complement(5327..5746)
                     /locus_tag="KPG09_002124"
     CDS             complement(5327..5746)
                     /locus_tag="KPG09_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966734.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSPINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(5979..6230)
                     /locus_tag="KPG09_002125"
     CDS             complement(5979..6230)
                     /locus_tag="KPG09_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966735.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGTKFINKKR"
     gene            6319..7050
                     /locus_tag="KPG09_002126"
     CDS             6319..7050
                     /locus_tag="KPG09_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBG0966736.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            7109..8020
                     /locus_tag="KPG09_002127"
     CDS             7109..8020
                     /locus_tag="KPG09_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0966737.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEVGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            8071..8883
                     /locus_tag="KPG09_002128"
     CDS             8071..8883
                     /locus_tag="KPG09_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBG0966738.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            8993..9685
                     /locus_tag="KPG09_002129"
     CDS             8993..9685
                     /locus_tag="KPG09_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0966739.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            9772..10713
                     /locus_tag="KPG09_002130"
     CDS             9772..10713
                     /locus_tag="KPG09_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966740.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            10750..11907
                     /locus_tag="KPG09_002131"
     CDS             10750..11907
                     /locus_tag="KPG09_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG0966741.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRHFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            12059..12619
                     /locus_tag="KPG09_002132"
     CDS             12059..12619
                     /locus_tag="KPG09_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG0966742.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            12647..13138
                     /locus_tag="KPG09_002133"
     CDS             12647..13138
                     /locus_tag="KPG09_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966743.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            13367..13918
                     /locus_tag="KPG09_002134"
     CDS             13367..13918
                     /locus_tag="KPG09_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0966744.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            14004..14936
                     /locus_tag="KPG09_002135"
     CDS             14004..14936
                     /locus_tag="KPG09_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBG0966745.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            14991..15854
                     /locus_tag="KPG09_002136"
     CDS             14991..15854
                     /locus_tag="KPG09_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG0966746.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFSNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            16101..17963
                     /locus_tag="KPG09_002137"
     CDS             16101..17963
                     /locus_tag="KPG09_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0966747.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            17966..19432
                     /locus_tag="KPG09_002138"
     CDS             17966..19432
                     /locus_tag="KPG09_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG0966748.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIAKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            19456..20292
                     /locus_tag="KPG09_002139"
     CDS             19456..20292
                     /locus_tag="KPG09_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0966749.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKKFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT         7659 a         2350 c         3357 g         7053 t
ORIGIN      
        1 tataaatttt caagaaagtg ctactttaaa taaatcttta gaaaaagatt ttctatacag
       61 attttatagg gttaaaaggt tttaatgaag aaaaaaattt tcagtgatat acaatatgta
      121 tgatgaagat atcatataaa tttgctatag ttataaatta attcgtataa aatataatat
      181 aaaacatatt ggactttcat ggaaaattag aaagcccaat atgttttaga gataaatctt
      241 atatttataa gttgcttaag atactagggt ctttaatctt attatcttca gcaagtaata
      301 agattttaga cataatctca gcagtcttag ggtcttcatc agcgaatgga aggaatatct
      361 tacctctact ttgagaatgt acaggaagta tattcaaagc acccatccct tttttatgta
      421 caataccaga acccatatgc acagaatact cacctaaatt acctgtgata agaacatgag
      481 agccttcaaa attgatattg ttaattttta ataataataa aagttcttta gcaattgcta
      541 tacgcatctc tacagtagaa tggctagttt cagggtcaac accaccagca tgagcgacac
      601 ttacaacaag gtctatatca cgcataactt ctgagaatag aattggaggt atagaagcta
      661 attctaaact tttataagtt tttctatcaa aaaattctac tgtttcaata gtaggaggct
      721 ctatatctgc tggtgaaaac caatctgcaa gtgcaaacat ctttgccact atattttctt
      781 tataaaatac tttctgtaat ccctcttcat aatcgacagt ccaaagctga tttctaagaa
      841 gtgctacagt tttctttggc tgaatttggt gaccagcata tctgcgagat atagtttttt
      901 cagatatttc atcagaattt attctataat actcacggaa tacttgctta aatggttgaa
      961 caagttgctt actaaataaa tcatgttgga actcactcca cttaccagat tcaaacaaat
     1021 gaactggatg tgcaagtttt aatttatttt ttgctgtcaa cttataagtt tcaccttcta
     1081 tattgcaaat aaagaactta gacttatcat tataaacata tccaatatta tcatcacata
     1141 cccatactag atttttaatc aaagggcaga taactggatt tttatagata ttggcaattt
     1201 ctttaactgt aaatactatt tcatttttca tagcttgttc taaactctgt tttgcacgag
     1261 aatactgact ctttaaatct ttttggacag atttaagttc aagtacatat ggatgtttag
     1321 caatagcttt tggtatactc tttaaagact tgttgccttt ttgaacttta gtagatgctt
     1381 ttccgctttc atcaacatct atccaaattg aagtaccatc aatatctttt ggtttaaata
     1441 gatgagcaac ttcatttagt ttttcagtct caagagccca catcatacga tttacatctc
     1501 caaatccagc acttctagct agattttcta gcgctatagt acaagctttt gattcacttt
     1561 cacgacgttg tgcaccaaat tgcttgcttt ctttagcaaa cttttgaata aattcataac
     1621 ggaaagttgc ttcttttagg tctccttttt tcataggaag taaagagaaa cttctcaatt
     1681 tatcttgatt tctatttgtt tcaatttctt tcatcaacat gtctttatca agcttaccaa
     1741 gtgtagcatc agcatagagt tgtgcacgtc tatgagagtt accaccacta gttatatatt
     1801 tagctgactt gtaaagtata ttgaatcttt cttccccaat agttttatat gaatctaaga
     1861 accaatctac atcaaatgca ccatcaatga aactacgtgg tgaaataggg gagaatagag
     1921 caacctctga ttctttttgt gcagagaagc cttcatttat atgtgcatgg aagtaccaaa
     1981 ttgtactttt cagacccttc cagtttaaat atttttcaat tatgtttacc cattgagggg
     2041 catacatagc agattctatt aatcttaact cagtaatgtc ggtattttta agtaaacttt
     2101 ttaatttttt tgaatcatct tctttagtag gatagcaatt tttaagtaga gaacttaaaa
     2161 catctttttt tgaataattg ctaccatatg aatatcctct ctggaaagtt tctttaccaa
     2221 gagcgaccaa aatatttaca aaataatcta taccttcaaa gtattgtata ttattagcta
     2281 gatgagacac ttctgttggc atatcacctc tacgtgattc aatttctata attcggtttg
     2341 taacagtttt taaattatct ttaagccaag ggtacttttc taatgtgtca ccagcccatc
     2401 taggatttgt taatgttctt attatatttg atgcgttgta tccacttatc atttctctat
     2461 taacttgctc ttcagtcatt aaacctaatt catgtgcacg agcaaacaca tttatgctag
     2521 tatagaaagg aaacttatat tctagcaact gataatttga aaacgtagtg tagaaaaact
     2581 tagtaaaaga aatatcatca taaataaacg aatgtgcttt atcaagccaa tagtttaaaa
     2641 taggggcatt taaagcaggg cagtattcat tttgtctgta atagttttta gtaaattcaa
     2701 ctctttgttt tttacattgt tctatagtca ataagtttct aagtttatag tatatttcta
     2761 aacaatagtt aaatatttct ttatggtcaa actcatttag taaaactctt aaaagttttc
     2821 ttgtaagtcc attaaattta aatttttcaa gttttgtata tattttattt tgttttttag
     2881 agtcaaggac atttgaatat atagatgaaa accaactttt ttctgtttca ttatatccac
     2941 atgtacttat actataaaat aagatatcaa gtattaattt gtaatctaag tcaagctttt
     3001 tactagtttc tctccaaagt tctgccaatg gataattatc aagagtatca ttagaaactt
     3061 tatatttaat aggagtcaag ttataagtag aataacctaa aagcactttg tctacattac
     3121 ctgaccatct ttcaacttca tattcataat ctgcattttc agtaataagc tcatcaaatt
     3181 tttcataaac ttttattatt ttatttatat ccgtatcgag aatttttttt gcttcttttt
     3241 ttgttgctac agaaacagaa tcctcactag tagataaact tatatttgaa ataattttat
     3301 taagctttga tgagctatta gaatcatctt taaaatcaaa ttgttctgga atttgaactt
     3361 tgttttcagg gttatatatt ccaaaaccat tttctttgca gtaatcattt tttgctttac
     3421 ctaacaaatt atcaattaat atttgaactt gagatgtatt ctttttagat ttatcaatat
     3481 cctctataag taaaataagc ttattatata gttcttgtct atcagaattg tcttttaata
     3541 agtttacaat ttcaagacct ccaaggcatt tttgagaatt tgaagatttt atcagtgaag
     3601 aagcaatatt ttccaaattt gaatcatctt gttcaagtaa aatttttatt actgtttgtc
     3661 taatagagct tgtctttaaa gacaatagat tggttatctt atctaattct acatctgtta
     3721 gctcaagctt tctaagtaaa ctcaataccg tttctctatt ggaaacactc ttatcactta
     3781 aacattccaa taaaaattca cgttgtctat ctgtctttgg ttcatttaca aaattgacta
     3841 taagtgaagc tcttaaatct gaactcataa gatgacaatt ttccataagc acatctataa
     3901 attgagcatt catatcgtat gctgttaaag tcatcataca tttaagtatt tcatgagaag
     3961 aaatttcaat ccaagtccat ggaaatacac ttcctgaaaa tgattttgtc tttgaattta
     4021 aaatttctaa agtatgtttt aaactttcaa attgttcttt tctagcatca aagtctttgt
     4081 caataggagt gtttgacaag tcaaaattag tttctctgtt ttgccaacta tagtagtagc
     4141 caacagaacc ttctacatag aaatttgtaa ttacccatgc tagtatctct aaatcatcac
     4201 ccttaatgtt tttagatgct atagaatgtt taaaatatgt actttctgta tgcgataaaa
     4261 agtaaagagc tacaagtttt ttatattttt cctgacaaga aagtaagttt tcaataagag
     4321 agtaagagtc atatatgttg taagttgcag tagcccataa ggcaaaataa atttcaagat
     4381 tatcattgct attaattgat tctatacgga acttttcatc agtcaatgat ttatatgaaa
     4441 gttctaaaca tttacgtatt attccaggtt tttggtctga aaaaccaagt cctgtccatg
     4501 tatccaaggc tcttataaca gaactaaatc gctctaaatt gttgtccaaa atagttttta
     4561 acatgtatat tgatgcagaa attgtaccct catctaaact ctctacaatt tgctgtctaa
     4621 gtccttcttg ttgtttggct gccaaaagaa gtttagaaag catttcatag catttatcac
     4681 tatgactctt catgatacca attatcattt gtcttgtaag tagagcagtt tgattttctc
     4741 caaatattac ttcttctaag gcacttgtta ctttttgatt tcctttgtct atttctagtg
     4801 ctattttatc actcattata gagtcatagt acaagacctt atttggaaga gtaagataat
     4861 ctataactga aaaatcataa taaattaaat caacatagtt tgaaataaac cttataatac
     4921 ctctaaaata atccacaagt ttatttgagc gatacccttt acgataatat cctcctgaat
     4981 atgtataatc catctttctt ttaattaatt ggcaaagttc ttctacttgt tcagaagtaa
     5041 atattaattc taatgcattt tttaaaggtc cagaaaggag ctcgtccata tttttaattt
     5101 cgtagtagtg gcaattgtag tatttcttta tcatatttaa gaatttgtcc tcaggttctc
     5161 tataatagta tgaactattt tctatgcagt cccaaagaga ttttgctaag tcgtagtact
     5221 gttttgaaga agatttagta aaattatttt taaaattttc aattatttta ggattttttt
     5281 ctactctagt acgttctaac atttatgtac ctccaaatta tttattttaa aacattcttc
     5341 cagcaagcaa ggtaagtctt ataaaagtaa atatatctcc acttttcaga tttatagggg
     5401 aatcgagttt ttcaataaca tcttcattta taagcacttt gtagattcca tcttcaaatg
     5461 cctgtaacgc tacttctatt gccttttcta cagattgttt attctcattg taaatagagc
     5521 caaatccaac ttttccaata tcagcttgag attcaatttc ttcattagtt aagaagttta
     5581 ttattgattt ttctgggatt ttctcattat attcaagaac atttttagat acaatttcag
     5641 agatagcttc ttttaggcaa ttgatgttgt cagatacatc aaaaggggta agttctaaca
     5701 taggtttgcg tttgccaaca gccttaactc taaaataaat ttgcataaaa tcacctcgca
     5761 aaatattaat tatgtattta gaaatttaaa atttatctat ttgttatatt atatcatttt
     5821 gtgaattatt aatgtgaatt atggaattat tttacaaaaa atagaaaaaa agctatctta
     5881 aagtctaaaa aagcaataaa agataacttt taactataat tatatagaat tataactgta
     5941 catttattaa attttaattt tagaattatt tttgataact atcttttttt atttataaat
     6001 tttgttccta aatatttttt gatatcttct tcaggagaaa agtcatcaat aagtatttct
     6061 ttcccagtgt cttcaaatac tacaagaact tcacagtcac cttgcaagaa aaattttcta
     6121 actgtatctt ctttaacttc tctaactttt attacattct ttttttcttt ttcaaagtct
     6181 tttgtgtaat aacttcccat catattaatt atttttgtat atctattcat aaaatacctc
     6241 catatgcgat taaatttaaa aaatatgtta taataattgt ttgtattaac ttaataatta
     6301 taacaaggag taataataat gactagtata aaaaataaag atattttaaa ttgtatagat
     6361 tattccatta aaaataagct ctttgacaaa ctaaatgatg tatataagtc cttgccagta
     6421 ggaaattgtt ctggatgtgg aaattgttgt atggagtcag taggtataaa tttaattgag
     6481 tttttgaata tattttgtta tcttgaagat agagttgatt taaaaaaaag ctgtataagt
     6541 aaaatattag attattattt tgaggagtat acaagaaaga acccatgtcc atttaaagat
     6601 actaataata gatgtctaat atatgaagtt agaccattaa attgtaggat gtttggacat
     6661 tggaaaaaag aggattataa taaaaattta gataatgtta ttgagaaaaa taataactat
     6721 agagagttaa tgaaaaagca atatggattt gaaataaatg atgaagtagt aaattacagg
     6781 atagattatt gtgatagatt tattccaaat aaagattatt taagtaaatc taagagatta
     6841 agcttttttg atgagcttat gatattagac tcaaatattt attcaagtgg aaatatagat
     6901 atagacttta gggatagagg tatagttgaa tattttatag agtcattact ttatagaaat
     6961 ttagcatata atgtgaaaat aaggatatct aaagaataca aaataagaaa aagaactata
     7021 gatagaataa aaagaattgt tcttgtatag ttttatatta gtaagtaata aattgtatca
     7081 taaatattgt caaaatgagg tgaggaaggt gaatcttaca aaagtaaaag gaaatacctt
     7141 ttttataaaa ggtggcacga atacaggaat atatgtattt gaagataaca cagcacttat
     7201 gatagaccca gggttatgtg gttcaagacc taggagagta aataaagtat tagatgaaaa
     7261 taatatacag gttagatata ttataaatac tcatggtcat gatgaccact atggagcttg
     7321 taatcaattt aaagaatatt ataaagatgt gtgtataatg tcatctgaat acgacaaatt
     7381 atatatagaa aatccagaat tgttttcaaa gtatattgtt gggggaaaga gcaatgtatt
     7441 tatggataat atcttaaaaa ataaaatgtt ggatgatata aagatagata aatctataga
     7501 agttggaaaa attgaattaa acaatgaaat gtttgatata attgagtttc agggtcatac
     7561 acctggtagt atagctgttt tgacgaagga taatgtgatt tttgttggcg atttattgat
     7621 aggagatgaa attctatcaa aatacgattt cttgtttcta tatgatatac aagaacaaat
     7681 aaattcatta gaaaaattaa aaaatataga ttttgagtac ttagttttag ggcatggcaa
     7741 gcaagttata tcgaaagaag attcttataa attaatagaa aaacatttga atgctttaaa
     7801 aaaatattta tatcaaataa gagagttgct tgtagaaccg aaatctttag aaattttatt
     7861 aaaaaacatt ataaataaca ataatttaag caataattat aaagagtatc atttttttaa
     7921 atcttcgtta gtatctgtca taagttactt agttgattta gaagaaatag ggtatatatt
     7981 ggaaaatgga gaattgcttt actatacaaa aacaaaataa attatgataa aataaagaag
     8041 gtcataaaaa acaaataaag ggtgagcaat atggaatacg aaaaatggaa tgagatacta
     8101 gcaccttatg aacatgcagt agaggaattg aaaataaaat ttaaaaatat aagaaaagaa
     8161 tatttagcaa aaggtgaata ttctcccata gagtttgtca caggcagaac aaaaaaaata
     8221 tcttccataa tatctaagtt aaaaaggata aatgcaaaag atatagaaac tgaaattgat
     8281 gatattgctg gtataagaat aatgtgccag tttgttgagg atatatatgc tatagttgat
     8341 ttgataaaag taagaaatga tatgactata ataggagaaa aagattatat tacaaattac
     8401 aaagatagtg gttatagaag ctatcatgtt ataataaaat accctataaa ctccattgcg
     8461 ggttcaaagg aaattatatg tgaaatacaa ataagaactc ttgcaatgaa tttctgggct
     8521 acaatagaac attcattaaa atataaatat gaccattaca tacctgagac tcttgctgaa
     8581 aggcttagaa gagcatctga tgctgccttt cttttagacc aagaaatgag tgaaataaga
     8641 gaggacataa tgaaagctca ggctatgtac caaatgaagt ctatagctat tagagatact
     8701 ctaaatagaa tacaagagtt atatgacttg ggtgatacac ataaagctat gcagtaccaa
     8761 agaaaacttg atagaactga aacagataaa aatataactg aaattttgga attaaaacaa
     8821 gaaatagata tattactaga gcaatataag gatactaagg tagatgataa agtagttcag
     8881 taaaaaacta cttgtagaaa atttgctaaa tattaatggg gctgcctctt aatggaaaat
     8941 aatttctaaa aagagacagc ctatttagtg taattaagaa aggaatgata ttatgagttt
     9001 gtatgctata ggtgatttac atttttcaac atcagtcaat aagccaatga atatctttgg
     9061 aagtaattgg gatggtcatg aaaagaaaat tatagataat tggaaagaag ttgttaagga
     9121 agaagatatg gtcttagtac taggagatac atcttgggga attaatttat cggaagctaa
     9181 aaaagattta gatataataa gcaaacttcc aggtcagaaa atacttataa aaggaaatca
     9241 tgattactgg tggacaaccg ttacaagttt aaataagcta tatgaagaca tgcgttttat
     9301 tcagacaaat ttttatgaat ataaagatta tgcaatttgt ggaggtaggg gctggatatg
     9361 tcctaatgat gttaaatttg atgagactga tgaaaaagtt tataaaagag aagagcatag
     9421 acttagacta tcattagaat ctgctcgtaa aagtggtcac tcaaaaataa ttgtgattac
     9481 tcattatcct cctacgaatg ataaattgga agaatcttta tttacaaagt tatttgaaga
     9541 atataatgtt gaaaaggtga tttatggtca cttgcatggt aaagaatctt ttaaaatggg
     9601 attgaagggg attcgaaatg gagtagaata tacattagca tcttgtgact atacagaatt
     9661 taatttaata aaagttcacg attaaaaaac ataaattggt aaatactata agatacatga
     9721 ttaaaaataa atatttgagg gatttcggtt ttgagaaagg agacacgact tatgaggtgg
     9781 atattagttt ttatattttt atatcttatt cctttagtag tgatattcag aaattacaag
     9841 aattttaaga ggtcttgtat ctacagtagt atttatgtgg tattagctac aacaataatg
     9901 ataagtaata tttatatgag tggacttaat aaaataaaag agtctatgta ttatcacaat
     9961 tatatttctg atgagacata tgatgaaaag tatgtatcaa attttaagga aaaagaaagt
    10021 accgatttaa aacaggataa taaaaagaat gaagaaatat caaataataa aaatttaaac
    10081 aacaaacaag atagaaatat acaaaataaa gaaagtaatg aagaaggaag taatgatata
    10141 aaagaaaaga ataattctga tagtaatgtt tcagtgaata ataaatcaga aaaacaagaa
    10201 gataatacaa aagaaaataa taaatataca agtaaagaaa aaaatagtga agatataaaa
    10261 aaaatagata tggaaagtat aggaattttt aagaaagaaa tttataatat agaaagagca
    10321 gctttagttc caatgagaga atgtatacct tacactaagg atatagcaaa aaatataact
    10381 aaattatcta gtataaaaaa agatgttaca aatgccagaa acaagtgtaa agaagttgta
    10441 aaagaatatg aaaatatgca gattccagaa ctttctaagg agaagtatac agaaacattg
    10501 agtaatgcta gagatgacgt aaagaaagca tatgagctta gagaaaaatc tatggaaagt
    10561 gctttgaaat taataaatac taaaagccct aaatatatag ggaagataac agaatatctt
    10621 gatttatcag ataagcagat agaaagtttt aaaaatagat tgaacgattt aaaagaagaa
    10681 ataaataatt cagaaacaaa taatattaat taataagatt taaagatata agttaaaagg
    10741 gagattatga tgaataaaca gagtatagga aaactaaata tgttttttat ggggattact
    10801 caaagaaacc ttgataatag gcattttttt attgagttag aggtgatttt taaatctgga
    10861 agaaaagagt ttaaaggaat tgcaatagag gaagataata aatataaatt taattttaaa
    10921 ggaaaatcag atttatatac gtttaatgag ttgcttaaga atatttctaa agaggcagaa
    10981 aattatgatg gattagtatt taaatatgtt gagcgaggaa ctattgttgt aattgaagga
    11041 gacaataaga aggtaaatgt aaaatattta gataataaag aagaagtacc taaaatagat
    11101 gaatttacgg catctcaaat aaaaaacaga gattattatg taaaagtagg acaagcaaat
    11161 gcacttttaa aagaaattgg agtacttaca aaagatggta agataaaaaa tgataaaata
    11221 agaaaataca atcagataga ccattttgtt gaattgatag atagtatttt aaaagaaata
    11281 aaggataaag actgtataac tatcttggat tgtgcttgtg gaaaatctta cttaagcttt
    11341 gtcttaaatt tttatataaa agaagtattg aagaaaaact gttattttat aggaatagat
    11401 tattcagatg tagttataga agcatcaaaa aatatggcta aaaacttggg ttataaaaat
    11461 atgtcattta taaaagagga tttgactaat tatacaccta atagggatgt tgaccttgta
    11521 ataagtctac atgcttgtga tacagctaca gatatggcaa taggtctagg aattagagca
    11581 aaatctgaag ctatagtagt agtaccatgt tgtcataagg agttattagg gcaatataga
    11641 tatgaagcta tggaaccaat attaaaacat ggtgtattta aagctagatt tgcagatttg
    11701 ataactgatg gtttaagaac attgttattg gagggcaatg gatatgatac ttctgtagta
    11761 gaatacatat ctccacttga tacaccaaaa aatttaatga taagagcgat aaaaactaaa
    11821 acgaataatg acaaagcatt aaaagaatat aaagaactga aatcacaatt tggagtggaa
    11881 ccaaccttag aaaaacttat atattagtta aaaatgtaaa atttacgaaa agcttgctta
    11941 aatatgcaag ctttttggta ttatataggt atagtcatat ccagagtttt gatttataat
    12001 taaagctgtg ctataatatt tgattaatca aatcaataaa tgttacatgg aggtaaaaat
    12061 gaacaaattg aatatatgta ttgatataga tggaacaata acaagccctt atcattttct
    12121 tccataccta aatgatatct ttaataaaaa tataactgaa gcagagtgta taacacataa
    12181 ttgggaagaa ttgtatggtt cgggaataag agatgtgtat gcggagttta ataataagta
    12241 tatacactca tatgatgaag caaagattgt agagggtgct acagaggtaa ttagcacatt
    12301 atctaaagga cataatttat cttttgtaac ggctagacat gagtgtttaa ctgatgtaac
    12361 taaaaattgg ctatcgagac aaggattttc agatatagat gtttacttat taggaagtga
    12421 ttataaagtt gaaaaggcaa gagaactgag ttgtgacata tttatagaag ataatccttt
    12481 aaactcagtg caactagctg atgatggtgt taaagtaata cttctggata ctaactataa
    12541 taaagatgtt aaacatcaaa atatagttag agttagtaat tggatagata ttgaaaagat
    12601 tataaaacaa ggtatttaaa gatttgtaga ttgaaagggg gagcatatga tagatttaaa
    12661 aattgaaaat agaaaaggaa aaatccatgt aaagagcagt gaaataaaag acattttaga
    12721 attacgtcca gacttcgaat atgtacaaga tatatcaaat actataaatc aagaaaatat
    12781 attggtattt gattgccagt tgacaggaaa ttcttttgat atggatgatt tagatataga
    12841 agaaatattg gaagagttag gtgaagaaat agatgagtct tatttcagtg ttttatttga
    12901 agatgtaaga gcgtatttaa aagatgctac tgatgagata gaagcagact tacaagataa
    12961 ttacttattt gataatttga gatgttattt tgatatatac aatataaatc aagaatttac
    13021 agattttaag tttgtatttt tagtttcttt taaagatata aaaatttctt cattagcaaa
    13081 cctagctaag attgtttcta agagacagct tataggtgca tctaagtttt atagttaaaa
    13141 tgaaatatta ttataataaa agctagtagc cttgtaaaaa tataggttac tagtttttta
    13201 tttgttcact ttcacaaaat ttcagaaaac tatttacatt ctaaaaaaag taatgtatat
    13261 taaacataat gtacagtata atggataaaa agtttctatt aaattactaa gtttatttaa
    13321 taatcaaaat aatttatctt atattagtat gtattagggg gtatttatgc aaagtttaaa
    13381 tgaaataatc gctgaaaatt taaaaaagat aaggaaagaa aaacatctta gtttagacaa
    13441 gattgcacaa cttagtggtg taagtaagag tatgctatct caaattgaaa agtgtgaggt
    13501 aaatcctact atatctactt tgaagaaaat aactaatgga ctaaaaatct catttacatc
    13561 attgatggag aggcaggaaa gtgacataga attgattcaa aaaagtgata tagaccattt
    13621 tattgaagat aatggaaagt atataagtta tccaatattt ccatttgact ccaaaagaag
    13681 atttgagata tttatgattg aaatagaaga aggtggaaat ttagattcaa atgctgaact
    13741 tccaggaaca caagagttta taactgtttt tagtggcgaa gtaaccatta aaataaatgg
    13801 agaagattac attgtatcta gtggaaattc tataagattt aaagctgatg tatcacatat
    13861 atatagaaac tcaggcaaag gtatagctaa aatgagtatg gtagtatatt atatataaat
    13921 aatttaatgg aattagagtg atatttagat gattttaggt atgaagatat attatattta
    13981 agttatagga gtgtgattta tggatgaata ataaaaaagc agtttttttc ttttgtagtg
    14041 atttaaagaa agacccagta gccagtaatg tgataaagtg ttgtgaagaa ataatggatt
    14101 taaatcaaac tgatattgtt attgatgata attttgtatt agaatttaag gataaaaaca
    14161 ataacttatt tcattttgta aaaacaaaag atgtaataag tcataattat aaatattatc
    14221 ttccaatttt aaatagacat ttcagagatt atgattttgc aggagttgta aattggcatg
    14281 agggagaaaa tgctccagaa catatcctaa cagctcatac aattggagat gttccaacag
    14341 gagaatttgg aaattcaaat ccaagatact ttaaaaatct aatcaatgct attgaggata
    14401 taaaaaatga aaatctactt gatgaattta ctacattaac agaagcaact cactggtctg
    14461 gaacaacata tggtgaagaa tctaaattaa ttacagaata ttctgtacct atgttggata
    14521 ttgagatagg aagtagtagt gatagtttta ataatagtat agcaatacag gtattggcaa
    14581 aatcattaat tagagtattt gattgtgatg aacctttgaa gactttatta tgtgttggag
    14641 gggtacattt tgagaaatct ttctcagata ttataaaaaa taaagaatat aacatatcaa
    14701 ttggacatgt attgccaaat caatggattg taagtggaat gtatgatgat gaatcgggat
    14761 ttgagaaact agaaaagtgt ataaatagta ttgaaggtgg aattgattgt atagtttttc
    14821 atgataaatt aaaaggtacc tataaagagc aatgccgtaa actaggtgaa aaacttaatg
    14881 tacctgtatt taaacataaa atattaaaaa atcccaaaga cttacctata tggtagtctt
    14941 atacagttaa tataacaata agtcggatta atttttaagc gaggtcaaaa atgatattat
    15001 cttattttgc attattaatt agagtagtgc tattagcttt tgaaagagta gttgtaagat
    15061 tattaggtga tgaagaagga gatatttata aaaatatagc ttcatcattt ctatttttct
    15121 ttataggggg agtatgttta cttccattta gcataatgga tagagttagt gattggtcgt
    15181 ttttaatacc atgttatata agtagtttag tctattcaat tggttcagta gcgtatgtaa
    15241 cgtctttagc aactggggaa gtttcacttg ttactccaat aaatagttta aattcattgt
    15301 ttttactgct tttatcagtt atatttttag gtgaaggttt aagtttagca aaagttgcag
    15361 gaatcataat tatgattggt ggagtgttta tattaaaaaa agtaagctct tttttaaaga
    15421 atgtctctgt aatttttaac aatttaccat gtagattaat gtttttatac atatttttgc
    15481 agtcagtagg aagagtattg gataaggctt tttttgtaca agtatctcct atattatatt
    15541 caactatact gtattttttc gtaggtttaa atttatttat ctttttagca tttaaaaaga
    15601 aacaaaaaat aatttgtgag atttttagta ataaaaaggg gctttcaatt ttaagtggtg
    15661 ctattaatgg atattcttat ttagcattat taattgcttt aaataatcta gaacttagta
    15721 tagcagagcc attttctcaa gtatctatga taataacttt aatacttgct cattttatat
    15781 tcaaagaaaa tataaaagaa aaaataccag gttcaatttt gatactaatt ggtgggtggc
    15841 tactgttact ttaattgaca taaaaaatga attataaagg tgctattagt tagtatcaga
    15901 aaatttaaaa aagtattgac aattctttta ggcaaatgtt aatataataa tacaaaatag
    15961 tataaataga agacatgtta ctggtaatgc aggcaggatc gaatatattg ctttgatttt
    16021 tcaaggtgtt tatataggtt ctgccttttt tgcatcttga atggccattc ataaaaaatt
    16081 atggagaagg agattacaag atgaattata aaaaaactgc cattgacatt attgaacaag
    16141 ttggtggttc aaaaaatatt ttagctttag aacactgttc aactagacta cgctttaagt
    16201 tgattgatag aagtaaagtc aatcaggatg ctttaaaaaa agtacaaggt gttatgagta
    16261 tagttaatgg ggcacaattt caagttgtca ttggaaatag tgttgttgat gtgtatgatg
    16321 aaattttaaa agtttgccct ttagatacaa atgatagtac tgtaaaaact gaagaaaaaa
    16381 taaacttagg aacaagattt atagaattta ttattaatat ttttcaacca ctgatttttg
    16441 ctattgcagg agctggtatt ttaaaatcac tgttgatgtt attagcaatg tttaatattt
    16501 taaaatcaga ttctactata tatacattat tagttgctat ttcagatgca acattctatt
    16561 tcctacctct tatggtagct gtaactactg ccaatgtatt gaaatgtaat cgtttagtag
    16621 ctattgccgc tgtaggatac ttattgcttc cagcaacaac aactgcttta agtgaaggtg
    16681 tcgaactatt tggatttaca attccaaaca ttgcctataa tgcacaagtt ttcccggcta
    16741 tcttatgtgt gtcttttttg gcaacaatgg agaaaatttt taacaagtat tcaccaaaac
    16801 caattcgaac tttctttgtt cctatgatgt ctttggcaat tactgttcca attactctaa
    16861 ctattttagg tcctttagga tataacgttg gtacaatttt tacaaccatt attttattct
    16921 tatataacaa aatgggattc ttagttgtag gattacttgc tgcttttctt ccatttatgg
    16981 ttgcaacagg aatgcacaaa gcactaatac cttattcaat taatacaatt ggaaagttgg
    17041 gttatgaagc tttatatatg acatcttcat tagcccacaa tatttctgaa agtggtgctt
    17101 gttttgcagt agccttgcga acaaaagatg aaacattaaa acaaacagca ttatctgcag
    17161 gaatttctgc cttgatggga attacagagc cagctttata tggtattact ttacaacata
    17221 aaagagcaat gttaggtgtt gtattctcaa gtgcaatttc tggtacattt ttaggattat
    17281 ttgcagtaaa aggatttgta attgttggac cagggcttgc cagtatgagt atgtttattg
    17341 acccaaacaa tggaaacaat ttgatttttg cgagtattgg ttttgttatt gcaatattag
    17401 gctcgtttat tattacactg attatttgga aggaagatac aaatacagta gagtcatcaa
    17461 ttggagatgt tgttgaagaa gagctagaag aagaaaaaga agtaaatatc ttagtaactc
    17521 cagtagaagg aaaagtaata gatttaagta aggttaatga tgaactattt gcaagtaaga
    17581 ctttgggaga tggagttgca attataccga ccaatggaaa tttatacgca ccttgtgatt
    17641 cagaagttgt gatgttattt gaaacaaaac atgcaattgg tttaagaaca aaaaatgggg
    17701 ctgagatttt gattcatatt ggaataaata ctgtaagtat gaatggagat ggatttaaaa
    17761 catttgttaa gacaggcgat aatgtaaaag aaggagattt attaattcaa tttgatttag
    17821 acaaaatctc acatgctaat ttagattcaa ctgttatgat tgtaaacaat aatggagcag
    17881 attatgctta taaagtgtta aaccagtctt atggcaatgt aaaaaaagga agtatcttgt
    17941 ttgatgtaaa gggaggaata taaatatgag aaagtttcca gaaggatttc tttggggagg
    18001 agctacagct gctaatcaat ttgaaggtgg ttggaattta ggtggtaaag ggtggtctgt
    18061 atcagatgta gcaaaggcac actttgatgc agatgtgaaa gactataaat ctaataatga
    18121 aatcacaaca aaagatatag aagaaggatt agcacatcct gaagatgaag taaattatcc
    18181 aaaacgtcat ggtagtgatt tttatcatca ctataaagaa gatattgctc taatggcaga
    18241 aatgggattt aagacatatc gaatgagtat tgcttggtct cgtatttttc caaatggaga
    18301 tgataaagag ccaaatgaag aaggtttaca gttttatgat gatgtatttg atgagttgat
    18361 aagttatgga attgaaccac ttgttactat gtcacattat gaaccaccat taaatatcgt
    18421 tttaaactat gatggatggt attcacgaca agttatcaat atgtttgtta ggtatgtaga
    18481 aactatctgt gaaagatata aaaacaaagt gaaatattgg cttacattta atgaagttga
    18541 ttctatgatt cgtcatccgt acactactgg tggtttagtt cgagaccgtt ttaaggataa
    18601 aaactttgag gaagttattt ttcaagctat gcatcatcaa tttgtagcta gtgcccttgc
    18661 aacaaaaatt tgccatgaga ttattccaaa ttcaaaagta gggtgtatgt taacaaaatt
    18721 aacgtattat ccatatacat gtagaccaga agatgttttg gcaactcaac aaaaaatgag
    18781 aagtatttat gcatatagtg atacgcaggt ttttggtgag tatccagtat atttactttc
    18841 gtattttaaa aataataaca ttcaaatagc aaaagaagaa catgatgatg aaattatgaa
    18901 aaagtatcca gtagatttta tttcattctc ttactatatg tcttcttgtg aggcagctga
    18961 tacaactgga ttagatatta cacctgggaa tacgttatta gctgtaaaaa atccttattt
    19021 agaaatgagt gaatggggtt ggcaaattga ttcaataggt ctacgtatat ctttgattga
    19081 attatatgat agatacagaa agcctttatt tatagttgaa aatggattgg gagctaaaga
    19141 tattttaact gaagataaaa aagttcatga ccaatataga attaattatt taaaagaaca
    19201 ctttaaatgt atgttagatg cgattatcga agatggcgtt gaactttggg gatacacttc
    19261 atggggttgt attgatttgg tcagtgaatc aacgaaacaa atgtcaaaac gttatggata
    19321 tatttatgtg gatgctgatg attatggaaa ggggacatat aatcgatata agaaagatag
    19381 tttttattgg tataaaaaag taattgaaaa taatagtata gattttaaat gaccaataga
    19441 aggtgagcaa ataaaatgaa ctacataata aagaaagttt tgaactcgag cgttgtttta
    19501 gttcatgatg caaagtataa tgaatttatt ttgctaggga aaggaattgg atatggtaaa
    19561 aaaacaggag aatatatttg tggttccaat gataatcaaa tgtttgtccc tgttgaaaat
    19621 acaaaatcaa aaaaattttt agaattaatg gatgatattc ctgtcgacat tttaaagata
    19681 acacaggaaa taatagtaga ggcagaaaag ttattgaact catcttttaa taaaaactta
    19741 tacttaatat tagcagacca ttttaacttt gcaattgaaa ggatgcgtaa aggaataaaa
    19801 attacaaatc gagtattttg ggaaataaaa aattattatc caaatgagtt taaggttgga
    19861 atggttgcaa tttatatggt agaggagaga ttgggcattc aattaccaga ggaagaagca
    19921 gctaacattg ctttccattt tgcaaatgct atggcttcag atggaaattc atatgatacg
    19981 attaagtatg ccaaagtaat tggagaaata attaatattt ttgttttttc tttgaataga
    20041 accttagata aaaagagtat gcattatatg agatttataa cacatataaa attttttgtg
    20101 gaaagatttt tttcagatag tatgttaagc agtggtgatg atttattatt taatcaaatg
    20161 aaaaggtcaa atccaaaaga aatgttaatt gcattaaaag ttagagattt tttagaaaag
    20221 aaatatggaa aaaagttaac aaatgaggaa atagctttct tggttgtcca tatcgcacga
    20281 gttaagcaat agagtgaatt actaatatta tcttatacgt ctacatggta gaaaattgaa
    20341 agaaggtaaa gtatataaag aaactgagac atattatttt gttgttcatt atgtttattt
    20401 tatgagtgta tcctgttaa
//
LOCUS       DAELSY010000068        20106 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.068, whole genome shotgun sequence.
ACCESSION   DAELSY010000068 DAELSY010000000
VERSION     DAELSY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20106
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.068"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            19..978
                     /locus_tag="KPG09_002140"
     CDS             19..978
                     /locus_tag="KPG09_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0966750.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDVALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            1292..1984
                     /locus_tag="KPG09_002141"
     CDS             1292..1984
                     /locus_tag="KPG09_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0966751.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            1994..2998
                     /locus_tag="KPG09_002142"
     CDS             1994..2998
                     /locus_tag="KPG09_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0966752.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGIHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKM
                     "
     gene            3246..4097
                     /locus_tag="KPG09_002143"
     CDS             3246..4097
                     /locus_tag="KPG09_002143"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG0966753.1"
                     /translation="MSLDVIFILKSVIIAIVEGFTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            4201..4947
                     /locus_tag="KPG09_002144"
     CDS             4201..4947
                     /locus_tag="KPG09_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0966754.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            4947..6863
                     /locus_tag="KPG09_002145"
     CDS             4947..6863
                     /locus_tag="KPG09_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0966755.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTYFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLIYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            7142..8059
                     /locus_tag="KPG09_002146"
     CDS             7142..8059
                     /locus_tag="KPG09_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG0966756.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYKSEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSIIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILSEIEKMKKIY"
     gene            8137..8874
                     /gene="cas6"
                     /locus_tag="KPG09_002147"
     CDS             8137..8874
                     /gene="cas6"
                     /locus_tag="KPG09_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG0966757.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KAYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKIVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            8880..10757
                     /gene="cas8a1"
                     /locus_tag="KPG09_002148"
     CDS             8880..10757
                     /gene="cas8a1"
                     /locus_tag="KPG09_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HBG0966758.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNLIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQIMFNDYLRQIIYFGELADLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKEYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTSINELFERNNSLKAQKSEYSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFTDKYANKKIQSIYDYKLK
                     SNVLDLLLKNRDLKHLTNNILRNKVKNDMESNNKSNISGIDCFRVVQLRALINSYKKG
                     VYKMDSKNLEKNDEKLRIIYYLGCDIHDYFVNKNSKNKIDGVSYKLLNSVKVGNKSDF
                     MDTIIRVFMSAEKQIPAFILDIEIEKDLDFESIGHAFISGLISGKYEGKDKLPNEKEE
                     K"
     gene            10759..11673
                     /gene="cas7i"
                     /locus_tag="KPG09_002149"
     CDS             10759..11673
                     /gene="cas7i"
                     /locus_tag="KPG09_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBG0966759.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGLADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            11675..12376
                     /gene="cas5b"
                     /locus_tag="KPG09_002150"
     CDS             11675..12376
                     /gene="cas5b"
                     /locus_tag="KPG09_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HBG0966760.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            12387..14708
                     /locus_tag="KPG09_002151"
     CDS             12387..14708
                     /locus_tag="KPG09_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG0966761.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQREFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIIGVGEANDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLADLTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFADSDSNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIHENKHTKQIFGKGVLISNEIDQFI"
     gene            14723..15274
                     /gene="cas4"
                     /locus_tag="KPG09_002152"
     CDS             14723..15274
                     /gene="cas4"
                     /locus_tag="KPG09_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HBG0966762.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEMLIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKEKIIENTLININEINMQLSP
                     PKIEKKHYCTKCAYYEFCFVKEE"
     gene            15277..16257
                     /gene="cas1b"
                     /locus_tag="KPG09_002153"
     CDS             15277..16257
                     /gene="cas1b"
                     /locus_tag="KPG09_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HBG0966763.1"
                     /translation="MYKDYYIISNGNIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNTLMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIVDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            16260..16526
                     /gene="cas2"
                     /locus_tag="KPG09_002154"
     CDS             16260..16526
                     /gene="cas2"
                     /locus_tag="KPG09_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HBG0966764.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITDGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   16710..18194
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=16710..16738
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            18384..18812
                     /locus_tag="KPG09_002155"
     CDS             18384..18812
                     /locus_tag="KPG09_002155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBG0966765.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIEPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(19268..20047)
                     /locus_tag="KPG09_002156"
     CDS             complement(19268..20047)
                     /locus_tag="KPG09_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966766.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMSSEVSSIRCCNCDELLEDITLNKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
BASE COUNT         8176 a         1821 c         3338 g         6771 t
ORIGIN      
        1 ttttaaggag tagagtaaat gaaattaaaa aagctattga taggtataac gatattaaca
       61 cttgcaacag catctttagc tggatgtgct aaaaaaaata atggagaaaa actttcagaa
      121 ataaatttaa cttatgtaaa atcaccatta aatgttcctt ctataattca gaaacaggat
      181 gatttatttg gaaaggaatt taaaaaggat aatataaaag ttaactttca cgaaattact
      241 acaggtccag agcaaacaca ggctttagct gctggagaaa tagacttctt acatgcacta
      301 ggtggtacgt cagctttaat agctgcatct aatggagtag agttaaagat attaaacact
      361 tatagcaggt caccaaaagg atttatgatt ttgactaata ataattcaat aaaatcagca
      421 gcagatttag taggtaagaa ggtggcaggt ccaaaaggga ctatattaca tcaggtattg
      481 atttcagcac ttgataaaga aggattaagt atggatgatg tggaatttgt taatatgggt
      541 attccagaag catcagcagc tctttctgat ggtagcgtag atgttgcatt gattgctggt
      601 ccagcagctc ttaaagctat gaagtcaggt agtaaacttg tagcaaatgg tgaaggttta
      661 gtagatggta taatagtaac tgctgttagc acagactttg cagagaaaca cccagaaata
      721 gttgaaagat ttatgaaggt tgaaaaggaa actttagaat atgtaaataa taattttgat
      781 gaagctatgg aaaaagtagc taaagaagtt gatttaagtc ttgaagaaac taaagaatta
      841 tatgcttggt atgattttag tttagatata acagataaag atatatcttc gcttgaagat
      901 acacaagact tcttaataaa aaataagtta caagagaaaa aagtgaatat aaaagaatta
      961 atctataatg ctaagtagaa actatgctaa aataatacta agtaagaaat ataataaaag
     1021 tttaagttac cttattaagg taactttttc tattttgaat tagtaatttc aatttatttt
     1081 agaatgatta gttgataata atgattaatt aatcataaaa agatactttt ttatagagtt
     1141 ttattaagat gtctaataat atggttatac ttatgaagtc agtaattgtg aagttaataa
     1201 tatgattaaa tttatatatt gtaaaaagat tataatatat taaaattgga ctgttatatg
     1261 atgagtaaat aggtatatta ggaggaatta tatgagttat aaagtatata ttgtggaaga
     1321 tgatatatca ataagtacat tacttaaaga ttatattgag aaatatggat ttgaagtgat
     1381 tgttgaagag aactttgatg atataatgat tacatttgaa agttttaagc cacacttagt
     1441 tctattagat gtaaatttac ctaaatttga tggtttctac tggtgtagat gtattagaca
     1501 acagtcgaat ttacctataa tttttatatc agctagagat ggcaatgtag accaaattag
     1561 agctttagaa agtggagcag atgattatat aacaaaacct ttttactatg atgtagtaat
     1621 ggcaaaaata aagagtcata taagaagggt atatggtgaa tattcaccta aaatagacga
     1681 aagaatagtg gagctagatg gattaaagtt ttatccagaa aggttagaac tagaattaaa
     1741 tggtgaaaat ctaattataa ctaaaaaaga aggtatactg attgaatgtc ttatcaagag
     1801 atacccaaaa gtagtaagta gagattatct tttagaaaag atatgggatg atattgagtt
     1861 tgtagaagaa aatactttaa atgtaaatgt tagcaggata aggaaaagat ttgaggaatt
     1921 aggtatagat aatgctgttc agactgtccg agggttagga tataaattaa ataagaattg
     1981 gtaaggagaa agtatgaagt tatttttaaa agattataaa ggttatattt ttatatatta
     2041 tataggcata gttatgacaa ttatttactg taatcttatg aagtttatta gttttggaga
     2101 gagtttatat attcttttat ttagttcttt tatattgtta tgttttttaa tatataagta
     2161 ttataaaaat agagaagtat ataatctgtt tgaaaaagga atacattctt tggaggagtc
     2221 atttttgtat ttaggaagct cattcttggg gagaaatata tcagttattt taaaacaaca
     2281 acataaattg tatcaatcaa tgatacaaga gcataaaaag atacatagtg aacatttaac
     2341 tttcatcaac caatggattc atcagatgaa aacaccaata tcagttataa gccttcagct
     2401 tcaagattat gagggagaag aagtaactga aaatataaga aaagaaatag atagattaaa
     2461 taaagggtta gatatggcta tgcattttgc aagggcagat aattttcaga aagactttat
     2521 agttgaaaaa gccaaattaa atgaaattgt atctagtatt gtaaatgaag aaaaaaggtt
     2581 atttataaaa agtggaatga ttcctaagat tcaagtagac aaatttactt atgtttatac
     2641 agatgtaaaa tggatgaaat ttataatagg tcaattaatt acaaatggag tgaaatattc
     2701 taaagattat agtaactacc taactataaa atctgaagaa aataataaat atgtaatttt
     2761 aagtataata gatgaaggaa ttggaattgt ttctaaagat ttaaaaaggg tatttgaccc
     2821 attttttaca ggagaaaatg gtcgtaagtt tggagaatct acaggtatgg gtctttatct
     2881 agtaaaaaag gtatgtgatg atttaggtca taaagtaagt ataaaatcag aagtaggaaa
     2941 aggtacagaa gtttcaatct actttaataa agatgtaaat agtttgataa agatgtaaat
     3001 tatctgttac aatagggttt ttagttaatt gtactttttt attatacaat ttattaaatg
     3061 aaatattcat agttaaataa tcaaaattta agagagttta tttgcaaacc ttacatagat
     3121 gtaaggtttg tgtaatgttt gcagatagtt aaattttcta atctaaatta caattaatat
     3181 aagaaataac atgagagaaa taacataaaa aaagaaattt tgtaaaaaag gaaggatgtt
     3241 tattgatgag tttagatgtt atttttatat tgaaatcagt cataatagct atagtagagg
     3301 ggtttacaga atttattcca gtttcatcta caggtcatat gattttggtt ggtaatttaa
     3361 tagattttaa ggggcaattt gctgaaatgt ttgaggttgt aatacaactt ggggctatat
     3421 tagcagtagt agtactttat tggaaaaaga taaaagatag cgttatagaa ttttttaaat
     3481 ttatatttac tggcggaaaa gaaggaaaga ttggttttag atttggaatg aatgtaatta
     3541 taggttgtat accatttgcg attataggag ttctatttta tgataatata aaatcattgt
     3601 ttaatttaca atctgtaatt ataggattta tagtgggtgg gattttatta ttagtagtag
     3661 aaactttgtt tagaaaaaag aatcattcaa cagataatat agataaaatt actccaatac
     3721 aagctttaaa ggttgggaca ttacaagttt tgtctgcatg gccaggtatg tcaaggagtg
     3781 catctactat tatgggtgga tggatagcag gacttaattc tcctacagca gctgagtttt
     3841 ctttcttttt agcagtacca gctatggtgg cttcttcagg aaaagattta tttgaatttg
     3901 attattcaat aatgacacca acattgtgga ttgctttggt agttgggttt atagtagctt
     3961 ttattgtatc aataatagtt atggaaaaat tcgtgaattt tttaaagaaa aaaccaatga
     4021 gagtgtttgc tgtatacaga ataatcatgg gtgtagtact tgcagtatta gcttttacta
     4081 acataattag tgtatagaaa aaatagattt attatctata aaaattaaac taagataaat
     4141 tataattaac aattatatat atgttatttc aaaaattaaa ttaggaagga tagttaaata
     4201 atgaaaattt taactgtaaa taatttaagt aaagtatatg gtaaaaaaat aattttcaac
     4261 gcattaaatg acataaattt tagtatagaa gatggagaat ttgtaggaat tatgggtcca
     4321 tctggtagtg gaaaaactac tcttttaaat atgatttcta ctattgacaa gccaactaca
     4381 ggaactatgg aattaaaggg aaagaatcct ttattgttaa gaggtgagga acttgcatta
     4441 tttagaagaa gagagttagg ttttgtattt caggatttta atttattgga tacattaact
     4501 ataggagaaa atatagtatt gccattgaca ttagataagg tcagtgtaaa agagcaagat
     4561 gagagattga atgaagtatc tacaatatta ggtataaaag atttattagg aaaaagaact
     4621 tttgaagtat caggtggtca agctcagagg actgctattg ctagagcatt gattaacaat
     4681 ccatcaatac ttttagcaga tgaaccaaca ggtaatttag attcaaagtc atcaaaggtt
     4741 gtaatggagc tatttcaaaa gataaataaa gaaaataaag ttactactat gatggtaact
     4801 catgacccat tagcagcgag ctactgtagt agaatcttat tcataaaaga tgggtctata
     4861 tataatgaaa tatataaggg tagtagtaga gagcagtttt atcaagagat aatggatgta
     4921 cttacattat tgggaggcga taactaatga atataaaaca gtttgctatt aacaatattt
     4981 caagaaatat taaagcgtat ttaggatact tagcaagtat tgttatatca tcttcactgc
     5041 tattttcttt tattatgttt acaagtcatc cagatttaga tgtatctcaa tttcctgatt
     5101 atttaaaaga aggtctcaag atgagtaaga taattgcata tctattctta ttcttctttg
     5161 tgttttattc agttagtgtg tttcttaaaa gtagatataa agaatttgga atactttata
     5221 ttacaggtat atcaaaacgc caagtaatga aattaatctt tatagaaaat atgataataa
     5281 atatagtatc ttctgtaatt ggaataataa taggcttaat ctttgcaaag atatttttag
     5341 tggttatgtc tactttttta gaattatcgc cattaaaatt ctatatacca tttaatgcga
     5401 tattgtctac tttaatctat tttatgattt tagctgtatt aacatcaata tttacttatt
     5461 ttatagttaa agaaaatcag gtgttgaagt tacttaaagg tacaaaaact ccaaaaccag
     5521 agcctaaaac atcactaatg cttgctatat taagtatttg tttgtttata atagcgtatt
     5581 ataatgctat tacctcaacc gaccaatata caacatatct gcgcttagtt ccagttacat
     5641 cccttactat agtagcaact tacttgttct tttctcaatt tagtgtattt tttatgaaga
     5701 agctaaaact aaacaagaga ttttatagaa aaaatacaaa tatgctttgg atttcgaact
     5761 tgatttataa ggtaaaggat aatgctagaa tatttttctt gataactata acttctgcag
     5821 tagcatttac tgctataggt acagtttatt ccttttggaa agatgtagag cgtcaaatta
     5881 atttaatata tcctaacaca atatattatt caacaatgac attgcataat gaccctaaaa
     5941 agcctgatag caaagaaaaa gacaaggaaa gaatgatgtt tatagaaaat aatctaaaaa
     6001 aagagaatgt agactataca aggactaatg gtgaatttaa aactgtattt cccaagaaaa
     6061 gtgattttaa tgtcagaatt atgaaagaat ctaagtactt agaaattaca gaaactatag
     6121 gggttaagcc tataagtttt gaagataatg aatgcatatc attattatca tcaagtttac
     6181 caggaaataa taacgtcaaa gaaaatatta ttgtagggaa tagaggttta aaggtggcaa
     6241 aacaagtgga agaatgtgtt atgccagcat attacaaaaa tatgtatgtg gtaaaggata
     6301 atttttatga tagtatagaa tcaaaattta tagtggatag atttactgca tttgaagtag
     6361 aggattcaag taaaactata gagatatgca gagaatttga aaataaattt aataatgagt
     6421 cagggatgca accatatttg tttttctcta aagaattaat gataggaact gggaaattaa
     6481 tatattctac atttatattt ttagcaatat ttataggttt aattttcttt gttactacaa
     6541 gtagtttttt atataataaa ttttatatgg attgccaagt tgataagaaa aagtatgagc
     6601 aattaaataa attggggatg acatataatg agattaaaaa agcttcaaca attgagattg
     6661 gaatagtatt tttgtttcct tatgtagtgg ctgttataca ttcaatattt gcccttactg
     6721 cactgaaaaa ttcttttgat atagaagtag catcatcagc tgttttggta atgggaagct
     6781 tgttcttggt tcaaatagtt tattttttaa ttgtaagaaa taattattta aaagagataa
     6841 ggttaaattt agtcaatttt tagttactat tattaaatga taataaaaag ggtttaaaat
     6901 taattaatat tactggaatt atagatgttt attttttcag taatattttt tattgtatta
     6961 actattaaaa ttttgtacaa atataaaata taatatggta aatgtcaaaa ttataaaaaa
     7021 attctactta tataaaatat aaagaggtta tatgtacata tgtgtagaaa taaataataa
     7081 aaatatcaat taatgttatt agggggggtt actcattagt aaattagcta atacacttag
     7141 aatgatgata ttaattcaaa gcagaggcaa aatgaaatgc aaagaattat cagaagagtt
     7201 ggaagtaagt gaaaggcaaa taaaaagtta caagatgtat ttagagcaag ctggtatatt
     7261 tataaattca acacctggga tttatggtgg ttatgaaata gataaatgta atagcatatc
     7321 attaattaaa ttattagata gtgaagtttc aattctagat atgataaatt cacagttaga
     7381 gtataataat gatatttaca aaaatgaatt taataatatt gtagaaaaaa taaaagcagt
     7441 attaaataca ggagaaaaaa gcgatacata tatggattat tttacagttc aagcacaacg
     7501 taattgtgat tataagtctg aaaaaaataa gtgtaatgaa ataattagag catataccac
     7561 aaaacacaaa ttttggatag aatattattc attaaattca ggaaatagtg aaagaattgt
     7621 tcatccatat ggtttattta attataaatc agatacttat atggtagctt tttgtgaaaa
     7681 aagatttaag tttatagact ttaaattatg tagaattaaa gattataagg ttttagaaga
     7741 aaaatataat gtagataaaa gttttagttg ggatgaatat agtaaaaata gtataggaat
     7801 ttataaagga gaagaaatta atgttgttat aaaaattagt catccatttt caataattat
     7861 aaaggaaaaa gtttgggtta ataatcaaca aatcatagaa tatgatgata aatctataat
     7921 gtttaaagct aaaatgagag gttatgagga gataaaaagt tggattttaa gtatgggagc
     7981 ttatgttgag gttgtagaac cagatagact tagaaatgat atattgtcag agattgaaaa
     8041 aatgaaaaaa atttactgat tttttcaaat aatttatact tggaccaata tacttcacgg
     8101 tctagtgtta atatattaat tgagagaggt gatattatga aaatgaaaat tgaatttagt
     8161 acaggttgta taccaatatc ttataattcg ctctttatga gtattataaa agaagcaata
     8221 agaaaatcta atgaagatta ctataaaaac atgtactatt ataaagaaaa gaataataaa
     8281 aaaactaaaa attttacatt ttcggtttat ataaaaaagt atagtattga aggtgacaat
     8341 tttattatag aagataaagt aatattaaat ataagtactc cagatttaga attgggtttt
     8401 catatataca atgggttaat gacttctaaa aaatgtttat ataaagccta tgaacttaca
     8461 agaattagaa tcgatttatc aagagaaaag aaggtaacag aagaaagggt actatttaat
     8521 gcattgtctc caatctgtgt aaagtctaaa gaaggaaaat tcttggagat aactgatgat
     8581 agatacatag aagaactaaa ctatataact aatgaagtgg ttaaaaatta taggggaaat
     8641 ggattaaaaa gaaagttaga atttgaaaat ataggtctca aaaagatagt agttaaagag
     8701 agcttaagag aatttaaaaa aataacaggt aaagagtatc aatatattaa tggttacaaa
     8761 ggtaaattta tattgaaagg tgatattgat gatttaaatc ttatctacaa tctaggaatt
     8821 ggttttagac gtgctcaggg atttggagat gtagatattt tagaatggag gtgagataaa
     8881 tgaagttaag agtttataga ggagattggt tttttaatat gggtatagtt ggatttttaa
     8941 atataataaa gaaagcagat aaacaagctg aaatatttat tatggaagat tatatcgaat
     9001 ttgatagctt atttcttgaa aactttcatg agtattattt taattacttt atggatgaat
     9061 atgatgtatc aaaaagaatt aaaaaaaata ttgattacag tataaatttt attaaatcta
     9121 agcctgatag aatcaaagat ggaattaaga aaattaaaga tagtgtgaaa caacaaaatg
     9181 ataagattaa aaaatttgat gaagaaaaat ttaacctgat aaaagagaag ctagatagca
     9241 tgtctaagat aaaaagttat gatgaatttg acagtttaga agctttagta gatgagacta
     9301 tagatatatt taaaataaaa tcaataaatg acaggcttac agcaaatctt tataaatatg
     9361 tagttcaaga taattatttt ggacaagtta gtttttttaa tgttgctaag gcgaaattag
     9421 atttagatgg attaaaacag ataatgttta atgattattt aagacaaatt atatattttg
     9481 gagagttagc agatttatta gaagagaatg actatgataa gttgaaaaac tatttaaatg
     9541 atagattaaa tagtatagct aaggatataa atgaaaaaaa agttagtaag tctagtataa
     9601 atactataga aaaaattatg aaggaaataa ataaaaactt tataaagaaa aataaaagca
     9661 tagaagatat aaaagagtat atggatagtt tagaagtatg tgaaatgtgt ggtttatata
     9721 agggaatatt agatgaatac tcagaaagta attttgctcc acttggagta agcaccaata
     9781 atgctaggaa tatgttttgg aatcaggcgt atgtaccatc aatatgtgat atttgtaagc
     9841 taatattgtt ctgtactcca gcaggggcta cttatacaag aaagaattat ttaattaatg
     9901 aagaaaatga gttttattta tttgttaaca tggatacttc tataaatgag ctttttgaaa
     9961 gaaataatag tttaaaagct caaaaaagtg aatattcaga ttccaaagat gaaaatcctt
    10021 ttaatcagct tataaaaagt atagtagaag aaaatacatt aaaaagtgaa tggcaacttc
    10081 gtaatatact ttttgtggaa tttaaagcaa gtatagattc taaaaaatgt aaaataaatt
    10141 attttaatat tccaacttat ttagcaaaat tttttacaga caagtatgca aataaaaaaa
    10201 tacaaagtat ctatgattac aagcttaaat caaatgtact agatttactc ttgaaaaata
    10261 gggacttgaa gcatctaact aataacattt taagaaataa agttaaaaat gatatggaaa
    10321 gcaacaacaa atcaaatata tcaggaatag attgttttag agtagtccaa ttaagagcat
    10381 taataaatag ctacaaaaag ggggtatata aaatggatag taaaaatttg gagaagaatg
    10441 atgaaaagct gagaataatt tattatttag gttgtgatat acatgactat tttgttaata
    10501 aaaattctaa aaataaaata gatggagtaa gctataaact tttaaattca gttaaagtcg
    10561 gaaataaaag tgattttatg gatacaatta taagagtttt tatgtcagct gaaaaacaga
    10621 ttccagcatt tattttagat atagaaattg aaaaagattt agactttgaa tctataggtc
    10681 atgcttttat atctggatta atttctggaa aatatgaagg aaaagacaaa ctaccaaacg
    10741 aaaaggagga aaaataagat gaaaagaggg ttaacattta cagtaatagc acaggctcaa
    10801 agtttaaact atggggaagg gataggtaat atatcagaat taaaaaaatt aagtagagat
    10861 gatggaaata tatatacatt tgcatctcgc cagtgtttaa gatatgacat agtacgatta
    10921 gcagcagaat tgtttaattg gaatttacag gttgtcgata aagaaaaagg tactattcaa
    10981 tttaaagaca atataagtat aagagaatca gaagaaatgg atttatttgg atatatgaaa
    11041 acaaataaaa aagatgagaa agataaaaaa ggaggttctt tgactagaga agctactgta
    11101 agattaagta atgcaatttc ccttgagcca tatagaagtg acatggactt tttaaataat
    11161 aaaggattag cagatagaat aggagaacat cctaatcttg ctaacataga gcaacattta
    11221 agttattata catatacagt gactatagat ttatctaaaa taggtaagga tggagatata
    11281 gaacttgata ataaagagaa atgtagaaga gtagtagaat ttttagaaat aataaaagta
    11341 ttgaatagaa atattagagg tagacaagaa aatttatctc ctctatttgt agtaggagga
    11401 gtatatgaaa tagcaaatcc atttttcttg ggtagaatta aattaaaagg agataaaaat
    11461 ggatttaaaa taaataagca agctatagaa gaagtaattc aaggtacttt tttaggaaaa
    11521 gatttaaaag aatttaccta tgttggaatg gtagatgggg tatttataaa taaagaagaa
    11581 tttaagggat tgtttgaaga taatttttta agtgtagata aattttttgg acaactagtt
    11641 aaagaagtaa aggaatatta tggggtgaat taatatgaag gttttgaaat tagatttatt
    11701 tcaagaaact gcatgctata aaaaaccatt tgctatgaag attacagaga catatccact
    11761 gcctccatat tctacaataa atggattaat tcacaaaata ttaaatgcta cagaatatat
    11821 accatttaat ataagtgtac agggaacata tgaaagtata tttaataatt accagacaac
    11881 atatttttat aaaaaagata gtattacatc tatgccaatg aatagtcata tgctattgaa
    11941 tgtaaatttg ataattcata taggtgggga ttttgattta ttggaagaaa tatatgatag
    12001 tttattaaat tatgacgagc acttatcatt aggtagaaaa gaagatttag taagaattaa
    12061 tgatattaga tttgtagaag tcaataaatt tgaagtagat gaggatatac aattagatga
    12121 ttatgaaaat tataagttat cagaatgtaa tataagaatg cctatatata taccaaaaac
    12181 aagtttacaa gatacagata tagatggtat aagttacaga ctcaataatt actatgataa
    12241 taatgctgtg gaagataaaa aaagggtttg gaataaggta gatagttatt atgttgagga
    12301 tggaaatact atatcaattg gagatatatt attagataat gaaggagatt tattatattt
    12361 taataatata aattaggagg gagagtatgt tatatgctaa atctaatcca gtggaaacac
    12421 ttagagaaca tactgatgaa cttttaaaac aaatgaatgt attaagagaa agttatggaa
    12481 aaaatataaa tagcttagat tttctagagg aagagatatt ttggcaatta cttgactttg
    12541 ttatagaatt tcatgatatt ggaaaagcat tttcaccgtt tcaagagtta ataaaaagca
    12601 gaatggatac taaaataaat gaacctaaaa tagtaactca tttagaaaat aatgtaggcc
    12661 ataattatct ttctcctgca tttattgatt atagttatat agacaggaaa aaaaataaag
    12721 aacttagagc agtattaaat caagttattg tatatcatca tgaaagagat atctttatag
    12781 ataaagattt taaaaattta atacaaaaaa tattagatga agatttaata aataaagttt
    12841 atgaactaca gcgtgaattt aaagttagat atccaataaa aacagaaaaa ttgagtaaag
    12901 tatatctaca aagtgtagaa aaaagaatag ataaaaatca caagtactat aatttatata
    12961 ttatgttaaa aggaatacta catagactag accatagtgc ttctgctcat gaagttgtgg
    13021 aatgcaataa tgttataaat ataggagaac agacagaaaa ttatttaatg aaagagtttg
    13081 gtagtttgag agaggctcaa tcatttgcta aatctaatag aaataagaat ataatactga
    13141 ttgcatctac aggaatggga aaaacagaaa ctgctttgat ttggatagat aaggataaag
    13201 cattttttac actaccatta agagtaagta taaatgcatt atttgatagg gctaaaaata
    13261 taattggagt aggagaagca aatgatacat tcttaggtct gttacattca acagcaatag
    13321 attatttaga agaaagtaat caggaaaatt caagtgagat tgtagattta gcaaagttat
    13381 tatcttgtaa actgacattt tccacaatag accaaatatt taaatttcct tttttgtata
    13441 gaggctatga aaaagtgtac tcaacattag catattcaaa agtagtaata gatgagatac
    13501 aagcatattc accagaaata gcagctgtat tggtaaaagg tatagaaatg atacataaga
    13561 taggtggcag atttatgata atgacagcta ctatgccaac catatatata gatgaattaa
    13621 aaaaaagagg tgtaatgaat agtaacttag ctgacttgac atgtaacaca gaaaaaataa
    13681 gacattgtgt gtctattgta gaaaattcaa tagatgaaaa cttaggtaaa ataatacaaa
    13741 gtggaatgaa taaaaaagtt cttgtaatag taaatacagt taaaagtgct gtagaaaaat
    13801 atgagttaat agaagaaata gttaaaccaa aaggaataga tgtcaactta aatcttttac
    13861 attctatgta tatacaagaa gatagagcaa aattagaaaa atatataaaa gaatttgcag
    13921 atagtgatag taatggtatt tggattacta cacaattagt ggaagcatcc cttgatattg
    13981 attttgatga gcttcataca gaaaattcaa cacttgacag tctatttcaa cgatttggta
    14041 gatgttatag aagtcgtgag tatgaagaaa atagcccaaa tatatttata tatacagaaa
    14101 aagctacagg aataggtagt atttatgata aggatattgt agaaaaagga cttgagctac
    14161 tgaaaacttt tataaatgga aaagaatgtg tgaagatgaa agaaaaatat aaggtagaaa
    14221 tggttaagat actatactct aaagaaagtc tagaaggtac agcttttgaa aagagattta
    14281 ccagtgcttt aaatatttta gatactataa cgccatatag tttaggttct aaagatgcac
    14341 aaaatatact tagaaatatt gaaggatata cagtaattcc agaggatata tataataaaa
    14401 ttgaagaaac tttaatcaaa gattatgaag agttgggaca agagcttata aatgcatata
    14461 gtaatgatga tagacaattg ataaataaaa taaagtctaa gagaaaaaaa gcaagaagag
    14521 agataattaa aaaaactgta aatttaccaa tatataaagc taaacaaaat gtaattgaca
    14581 tacaggtgaa aggtcttgaa gatttaaaaa tacttttata taaatatgat atacacgaaa
    14641 ataagcatac aaaacagata tttggtaaag gtgtgcttat aagtaatgaa atagaccaat
    14701 ttatttgatt tttatattaa caatgtggct aaaatttact aaagaaggtg attttgtgcc
    14761 aatagattta gaagtattaa aagttcaagg cgtaaaaata aattattatt atgtatgtaa
    14821 aagaaaacta tggctatttt ctaaaggaat aactatggaa caaaatagta gtagagtatt
    14881 gtctggaaag atagtacatg aagattcata taaaagaatg aaaaataaag aaatgttgat
    14941 tgatgatatc ttgaagctag atataataaa ggacgagtat gtaaaagaag taaaaataag
    15001 ttcaaagatg aaagaagcag atgaaatgca gttatactat tacttatact atttaaagaa
    15061 tagtatgggt atagagaaaa aaggaactat aaattatgtt aaggaaaaga aacaagaaga
    15121 attgatattg acagaagaaa aagaaaaaat tattgaaaac acacttataa atataaatga
    15181 aataaatatg cagttatctc ctcccaaaat tgagaaaaaa cattattgta caaaatgtgc
    15241 ttattatgaa ttttgttttg tgaaggagga gtaacaatgt ataaagacta ttatattata
    15301 agtaatggaa atataaaaag gaaagaaaat acgatttact attacaattt agaaggtgag
    15361 aaaaaaatat taccagtaga acaagttgat gatattcata tatatggaga agtagattta
    15421 aacacaaaac ttataaatta cataagtaaa tatggggtga tgcttcattt ctacaattac
    15481 tatggatatt attcaggtac atattatcca aggaagaaaa atgtatcagg attcagttta
    15541 gtatgtcaat cagcctgcta tttagataaa aatgaaagat tatacttagc aaaatccttt
    15601 gtggaaagtg catcatacca tattttaaga aatatgagat attacaaagt tgatgaaagt
    15661 ttaattgata ctataaagga tgaactaaat gatttgaatg atgtcaaaga aatatcagaa
    15721 cttatgggta aagaaggcag aataagaaaa acatactata aatcatttaa tcaaatatta
    15781 aaagatgatt ttgaatttta taaaagagaa aaaagacctc ccaaagaccc agtaaatact
    15841 cttatgtctt ttggaaatag cataatgtat acgaatgttt taagtgagat atataagaca
    15901 caacttgacc ctacaataag ctttttacat gaaccatcaa caaagagatt ttctttaagt
    15961 ttagatattg cagaaatttt taagccaatt atagttgaca caataatttt tagcctgatt
    16021 aataataaaa gaattaataa gaatgatttt gaatatcaag acaaaatttg ttatttaagt
    16081 gaaaaaggaa agaagaaatt tttgcaagaa tttgaaaata agatgcagac aactataaaa
    16141 catagaaatc taaatagaaa agtttcatat agaatgttta taaggttaga gtgttataaa
    16201 ttaattaaac attttataaa tgatgaaagg tacaaagttt taaaggtgtg gtggtaagta
    16261 tgtttgttat tgttacttat gatattgttg aagcaaggtc gttaaataga attagaagga
    16321 tacttagaaa atatttgact tggacgcaaa attctgtttt tgaaggcaat attactgatg
    16381 gaaagttaca taaatgtatt tctgaaatag aaaatattat tgataatagc gaggattcaa
    16441 tctatgttta tgagataaaa aatcctaatt caattaaaaa gaaatgttat gggattgata
    16501 agtattctga tgaaatgttt atataggttt gcagtgagcg atatttatgc taaaataggt
    16561 gttaacagtt ggaatataag ggattgaagg tgtatgataa ctgttatcaa ttgcactact
    16621 gctcgctcac tgcaaatttt gatgttttta ttgaattata attgcttgat tgaagtattt
    16681 tcaatgtatt caattatacc tattttgggg ttttatatta actaagtggt atgtaaattt
    16741 aaaggtgcaa gtcttaatac ttgttacatg agttgtttta tattaactaa gtggtatgta
    16801 aataacaaag aaccatttga atcacgaaaa ctaaaaaaca gttttatatt aactaagtgg
    16861 tatgtaaatt tattaacctt atcagaaacc tgaccatccc aattatagtt ttatattaac
    16921 taagtggtat gtaaattcca tttgtctaat gcttctaact ctttttttat acgttttata
    16981 ttaactaagt ggtatgtaaa ttccaaaatg ggcttggagt taagagttgt ataagaaacg
    17041 ttttatatta actaagtggt atgtaaatta attacacact cccttttaac gaacatattt
    17101 tagattgttt tatattaact aagtggtatg taaatagact cgacatagaa tcggttacta
    17161 aatccgaggg tttcaggttt tatattaact aagtggtatg taaatactaa acctttttgt
    17221 ttacttttat ttctcatttt acgttttata ttaactaagt ggtatgtaaa tccggatttt
    17281 tctgcttcga ctgttgttat tgagacttgt tttatattaa ctaagtggta tgtaaatagt
    17341 aattccatgc aatcttttat attgcttttt aacctgtttt atattaacta agtggtatgt
    17401 aaatagatat acaaggtgaa agtgttgaaa tttcaatctt gttttatatt aactaagtgg
    17461 tatgtaaata ttcttagatg ttataatttg tgttgcattt aaatcgtttt atattaacta
    17521 agtggtatgt aaatacaata ttattgttca tttcatttaa taattctagt agttttatat
    17581 taactaagtg gtatgtaaat ttgtttatac ctttaatctt taaaattaaa tataaaagtt
    17641 ttatattaac taagtggtat gtaaatcatt gtatactaag cttgatatag tgcaaaatga
    17701 aacgttttat attaactaag tggtatgtaa atactaattg ggttaaacaa atggatataa
    17761 ctaaattagt tttatattaa ctaagtggta tgtaaatcca acccttcgca cgaatccgtg
    17821 cgacgaaata taaagtttta tattaactaa gtggtatgta aattgtaaaa atattgctta
    17881 gcatattgat atcactaact gttttatatt aactaagtgg tatgtaaata caagaggttt
    17941 ttcagcaata tatgtacttc tataatgttt tatattaact aagtggtatg taaattcatc
    18001 aatattaatt ttgggaatat caacattttt tgcgttttat attaactaag tggtatgtaa
    18061 atgcgataag agattcgagt ctttttactg ggcttaatcg ttttatatta actaagtggt
    18121 atgtaaatag gataaagaaa agactcacac aagacacagt gtcaggtttt atatcaacta
    18181 agtggtagat aagtagaata ttagcaatat caacgagtat ttagaaactt attttatatt
    18241 aataaagcag tatataaaat tgtagaataa acaatagcat atactaaaac atccacctta
    18301 tattaactaa atcatatcta aataaaatgc acttacctat aaacattata aaatcaatac
    18361 aaaaatgagg tgaaacaaaa tttatgataa agaaattaaa caataaagac ataaataaaa
    18421 tcatggaaat atgggaaaaa agtacaatca aagcacatga ctttataagt aaagaatact
    18481 ggcaaaataa ctacaatact gttaaaaacg aatatatacc tatatcagat acatttgtat
    18541 atgatgatgg agatgaaata aaaggattta taagcataat agataaaagc tttataggag
    18601 ctttatttat agaacccaaa taccaaaatc taggtatcgg aggtaaactt ttagattatg
    18661 caactaaaaa atataaaagt ctaagtttag cagtatataa agataataaa aaagcagttg
    18721 tgttttataa taaaaaaggt tttaatatag taaaagaaca agtaaatgaa gattcaggat
    18781 ttaaagagta cataatggaa tatagtaaat aatatgatta cattctatta aaagaataat
    18841 taatttattt tctatttgat ttttataaga gtaattttta aaatagacta tatgaagata
    18901 aagatattta ttatagatgt aagttaatca gaattgatat agctacaaaa gaggaaaata
    18961 aaattgtatg gtgtatttca gatttaacaa atttactatt taaagataat aagtttgata
    19021 taatattaaa gatactttca cctataaact atagggtatt tgaaagaatt ttgaataatt
    19081 atggaatgct aataaaggtt tgttaaaatt taataattat tagattatag aaaattgcca
    19141 tgaaattagt tcagcaagaa ctagtttcat agcaatttta attcttataa gtgtatttat
    19201 ttttacagat ttaaaaaact tatacagtat ctaataatgt aatcttatta ctttagccta
    19261 taaatattta aatcttaaaa tgttctcttt gagataagtt tatttcagag tcacaatttc
    19321 cacaacagta agatgttgag acatctttaa atttttcagt attctcatca aagttcattt
    19381 ttttctttgc aataattcct accttagtta aattagaacc acaattatta cagttttgaa
    19441 ttttacatag gttatatagt ttattaagtg taatatcttc taataattca tcacaattac
    19501 aacatctgat agaagaaact tcactagaca tttcaatagg ttcagcttct ttagaagaaa
    19561 atttatatgt tatgtcattt gatactttat aacctgtatc aaatatatta gaattacaac
    19621 ttggacataa gtactttgga agatatatat cttttttttc aaaagtatca ctactaatat
    19681 tacagtttaa ttcatctaaa attggtcttt catctaggtt attaccttta tttaagctta
    19741 gaagataatt ataaattttt ttatcttcat tctgttttga tgtatctaac aaatgctcca
    19801 ttatattagt gtattttttg ttttttgaag gcatgtttaa atccagataa ttgttttcaa
    19861 tagcaaagca tactatattg aaatagttat atttaactgc aaaaaataat aacatttcat
    19921 caaatacttt atctattatt ttgtcagaaa tacttatcca aaaatctggt gactctaagt
    19981 aaactttcct actaagttca tctaactctt catctatatt atttatatta ttttcattaa
    20041 ctttcatatc atcacttcct tttaaatatc ttgtgtataa actatttgtt actatttttt
    20101 atataa
//
LOCUS       DAELSY010000069        19620 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.069, whole genome shotgun sequence.
ACCESSION   DAELSY010000069 DAELSY010000000
VERSION     DAELSY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.069"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            169..537
                     /locus_tag="KPG09_002157"
     CDS             169..537
                     /locus_tag="KPG09_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBG0966767.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNKVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
     gene            962..1207
                     /locus_tag="KPG09_002158"
     CDS             962..1207
                     /locus_tag="KPG09_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966768.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
     gene            1306..1569
                     /locus_tag="KPG09_002159"
     CDS             1306..1569
                     /locus_tag="KPG09_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBG0966769.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(1854..2801)
                     /locus_tag="KPG09_002160"
     CDS             complement(1854..2801)
                     /locus_tag="KPG09_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBG0966770.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALATEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(2804..4435)
                     /locus_tag="KPG09_002161"
     CDS             complement(2804..4435)
                     /locus_tag="KPG09_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG0966771.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            4565..5482
                     /locus_tag="KPG09_002162"
     CDS             4565..5482
                     /locus_tag="KPG09_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0966772.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            complement(5539..6120)
                     /locus_tag="KPG09_002163"
     CDS             complement(5539..6120)
                     /locus_tag="KPG09_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0966773.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(6160..6726)
                     /locus_tag="KPG09_002164"
     CDS             complement(6160..6726)
                     /locus_tag="KPG09_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0966774.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            complement(7181..8041)
                     /locus_tag="KPG09_002165"
     CDS             complement(7181..8041)
                     /locus_tag="KPG09_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0966775.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            8190..9080
                     /locus_tag="KPG09_002166"
     CDS             8190..9080
                     /locus_tag="KPG09_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0966776.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLSPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            9298..11052
                     /locus_tag="KPG09_002167"
     CDS             9298..11052
                     /locus_tag="KPG09_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBG0966777.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDVTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNVKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVSVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLVGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
     gene            11436..12422
                     /locus_tag="KPG09_002168"
     CDS             11436..12422
                     /locus_tag="KPG09_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0966778.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPMVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            12645..12728
                     /locus_tag="KPG09_002169"
                     /pseudo
     CDS             12645..12728
                     /locus_tag="KPG09_002169"
                     /inference="COORDINATES: protein motif:HMM:NF012587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            12759..14240
                     /locus_tag="KPG09_002170"
     CDS             12759..14240
                     /locus_tag="KPG09_002170"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBG0966779.1"
                     /translation="MYKEPKYRTILESIKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSVLEIFI
                     NDGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            14266..15237
                     /locus_tag="KPG09_002171"
     CDS             14266..15237
                     /locus_tag="KPG09_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBG0966780.1"
                     /translation="MKKVISIGEALIDFIPNQNGCKLKNVSEFRRVAGGAPANVSVVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFMTGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(15493..16095)
                     /locus_tag="KPG09_002172"
     CDS             complement(15493..16095)
                     /locus_tag="KPG09_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0966781.1"
                     /translation="MEICILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEKHSRQFAKQIYYILDNSIEVDNC"
     gene            16376..17587
                     /locus_tag="KPG09_002173"
     CDS             16376..17587
                     /locus_tag="KPG09_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBG0966782.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKYDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIVFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            17655..17852
                     /locus_tag="KPG09_002174"
     CDS             17655..17852
                     /locus_tag="KPG09_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBG0966783.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            17886..18857
                     /locus_tag="KPG09_002175"
     CDS             17886..18857
                     /locus_tag="KPG09_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0966784.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            19044..19421
                     /locus_tag="KPG09_002176"
     CDS             19044..19421
                     /locus_tag="KPG09_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0966785.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         7613 a         2365 c         2973 g         6669 t
ORIGIN      
        1 aaagtaactt tatataaaaa gtaatataaa ttgataaaat tataaattta tttatagtga
       61 atataggttg gattttatag atacaaaaat agtatatatg atatgtagac cattagcatg
      121 tttaaagtag caaaacatat aaaataaaat ttgaaaggaa gtataaaaat gacaagacca
      181 tcaatatatc atagccaagg atacacttgt gcagaggcat taataaagtc ttataatgag
      241 gaacacaaca cagatatacc aatctcaata ggaagtggta tgggggttgg aatgaatgtt
      301 ggaagtgttt gtggagctgt aaatgctgct gttgttataa tagggtattt aaatgggcga
      361 aatagtaatt tagatgaaaa tatagcaagg aagtattcaa gagagctatt gaataaagta
      421 agaaataaat acagctcaga aatatgtgta tctcttaaaa aaaataaagt aacttgtgaa
      481 gaaataataa atttcacata tgatgctcta aatgatatgc taaaaaatca aaaataattc
      541 taagaaacaa gtattttaca ttcgatagaa tacttgtttt tttatgatat ttttaatagg
      601 tattagttta ttataatttt taaatcaatt ttattatatt ttaattgaaa taatctactt
      661 tgaaaattac taactgacaa aagaatatga ttatagtata gaaaaaatgt aaattattta
      721 atattaagtg agagtatatg cttacctata taaaatcgta taaattaatg gctaaattta
      781 taaattataa agaaatatat aagaaaacaa aagaattaat tagtataata taaagttaaa
      841 aagtgaaaat ttatataatt tatttataaa gtaaagaaaa aatagaaatt tgtaatgaaa
      901 taaaaagttt atgacataat atggtataat tagtaaaaat atagagattg gaggaattgc
      961 tttgtttata gatgaagaat tagaagggta tatattaaca tgtaaaatat ctgaagactt
     1021 taaaaatata cctgaatata gtgatgaaga gttttatgtt acagtctata aagatgaaag
     1081 ttctgactct gggtactatg ctttattaga aaataaagaa gaaagagttg tatgggatgg
     1141 agaagttgtt gccaataata tttttaataa cctttggatt gtagtaaata aggttaaaac
     1201 tggataatag tctaattata tagtatttgt aatattttaa atgaattttt acatatttga
     1261 gatgagtaaa taacaaagat tattcgatac tggaggagta attcaatgga atatagttat
     1321 agtaagatga acttaaaaaa aggggatatt gtagaggtaa atttagagaa acaagccaat
     1381 gtaatattgt tagaccatat aaattatgtg aaatttaaaa atcaaagaaa ttatgattac
     1441 tatggtggat ttgcaaagaa aaatccatgt agaatgagag ttcctaatac tggaacatgg
     1501 tatctagtag taaatcaaga tggaaatagt ggaatagtaa acttttcaat caatacaatt
     1561 caaaattaat tttagaatat tggatattct tactttttgt actttttata tagttaaaat
     1621 aaaattaata agcaattatt ataaaataat gtgggtgcct caaaatgaat tttataattc
     1681 acgagacact cttttttgta taaactcaaa tatactttat catttcaaca atgaaaaaga
     1741 ggcgtatctg tgttttgaga tagcctcatt tatcatatat catatataga attttaatta
     1801 ttcttaacta aaattttttc cataggttat ctgtacaatt aaaatattta ttgttagcat
     1861 acagctccac ctgtaaggtg taaaagagcc tctagtatct cttcttttct ttccaaaact
     1921 tcatctgata aaagcatttc tataaattta tcagcaccaa ttctatcgat agttattcca
     1981 aaacgttcac ctgtgttacc ctgttctcta aataaaagaa ttgctttttc aattaaagtc
     2041 ataagttcct cttttgaaaa taatctatca atctgtgttc caggacgaac tgattttccc
     2101 catttccctc ctatatagac tttataacca gattcttttt cttcaatact atcaaaatta
     2161 caactttcta tacacttacc acagttatta catagattac tgtctatttc aagtttacct
     2221 ttatctgtta atttagctgc tttaacagga cagacctcta caacagcaca ttttttacaa
     2281 ccaacacaaa gctctgaatc atagtcaggt acacgttgac caacaattcc aagatcgttt
     2341 aggtcaggtt taatacagtt attagggcat ccaccaacac caattttgaa cttatgaggt
     2401 agttttacat cataccaatt tttgtaaaat tcttcgtgta tttcagtagc aagtgcctgt
     2461 gtatcagcta gaccatgaac acaaacagta cctttacaag ccacaacagg acgaaccctt
     2521 gatccagttc cacctgttat tagattttcg ctagcaataa attcacttag agcttcaata
     2581 tcttcaaatt ttacaccagg taattctact gttagtctag tagtaaatgc taactgaccg
     2641 tttccgaatt tttcagcagc ttctgcaatt ttttttgctt gtgatgcatt tattacacca
     2701 tttacagtta ttatacgtgc tgcaaaatgt tctccatccc tgcttggtag aaaacctcta
     2761 ccttttactt cctttttttg ttctgctgta acattactca tatttactcc tccattacct
     2821 taagatttgg ataaaatagt attccacctt caagaacttt cgtatttgta tagccaaagc
     2881 gtcttaagcg attttgcgcc atatagctat ttttaccttt agcacatata agaagaattt
     2941 tttcatcctt agcaagacct tcaatttctc cattaatttc accaacagga atataacgta
     3001 aactaggaat agaaggagct tttgaaacat caagtttagt ccaactgtca aaatcctcaa
     3061 tttcgtcaag taaaacacta tcaagttctc cgttaagttt attttgtaaa acatttactg
     3121 ccactacaaa agggtgaata gctgttgaaa aaggtggtgc atatgcaagg tccatacttt
     3181 ctaatgaata aaggtcagcg cctaaagtta ttgcagtagc agcgatatct actattttat
     3241 caaccgctcc aggtcccata acttgaacac ctaataattt tctagtattt ttttcagcaa
     3301 ccattcttat aataaaatta gatgcacctg gataataatg agctttatca tcggtagcta
     3361 tggttacaga gcaaacatta taaccttctt tttcagctat ttctttactt agacctgttt
     3421 ttcctgcatt gagctcaggt aattttacta ctgtcgttcc aagaacacca ttgtaagttt
     3481 tagattttcc agatatattt tgtgcgcaaa ttctaccctc atgatttgca gatgacccca
     3541 taggtgacca tgtaggttta tttgttaaag aattttttac aaaagcacag tctccaacta
     3601 cgtatatatc tttatcattt gtaagcatat attcatctgc aataattgta ttattagggg
     3661 caaattctaa acctgtgtct tttagaaaac ctgtgttagg acggattcca actgacataa
     3721 ccactaaatc tgttttaata gctctatttt tagtacgaag tttttcaact ttattttcac
     3781 cttcaatacc tacaacctgg tcacctgtaa aaatcattat gcctttatca gccatatgat
     3841 tttcaacgta ttcagcaaaa tcagtatcaa atccaggcat aacatgttca gccatatcaa
     3901 caacacttac acgtataccc attgcagaaa gattttcagc aatttcaaga ccaatgaaac
     3961 ctccaccaat aacaacagct ctcttaatac cgttttcaat agaatctctt agttttatag
     4021 catcatttgg agtacgcata aagaatactc caggtaaatc tattccaggt attggtggtt
     4081 ttattgggtc tgcccctgta gcaattacaa gtttgtcata gcttagattt tctttttctc
     4141 ctgttgaaag cgttttcaaa gttacttgtt ttgcatttct atcaatagac gtaacttcaa
     4201 ctccacaacg aacttctgct cctgttagtt tagaaaaact ttcaggtgta tttacgatta
     4261 aagatgattt atcttcaatt atcttaccaa cgtaatatgg aagaccacaa cctgcataag
     4321 aaatatcatc acctttattt agtattgtaa cagagcaatt atcacccatt tcacgtttta
     4381 tttttgcagc agtttttgtt ccagcagcaa ctccacctat aattaatact ttcacaatat
     4441 atcacactcc aatctacttt ataactctat aataaaatta tacttcttga caatatattt
     4501 gtaaaatact aaaatattaa taaaatcata agcatttact aatgtttgct aaggttgtga
     4561 taatatgaca attcgtagtc ttgaaatctt tgttaaggtt gctgaatgtg gtaaaatgag
     4621 tgaagtagca agaaatatgt atattaccca gtcatcagtt agccaagcta tttcagaaat
     4681 agaaaaagag tatggtgtta agttatttga tagaatttca aaaaaattat atttaactga
     4741 ggcagggaaa aaacttcttg gatatgggag gcatctttta gctgtaaatg aagaaatgaa
     4801 tgattgtatg aagcattgtg caaaaaatat ccgtataaga gttggggcaa cagtaactgt
     4861 tggtacatgt gtaataagcc cgataatgtt agaattatac aaggtaaatc ctcttataga
     4921 acctgaagta tttgtagaag atacaagact gattgcaaaa aaattactta atagtgaatt
     4981 agatattgcg attgtagaag gaaaaattaa gcacccagat attgttacaa aaagcattat
     5041 taatgataat cttgtactta tatgttcaca caagcatgag ttctacaaaa gagactctat
     5101 aaaagtttca gaactttcaa atcaaccatt aattatgcgt gaattaggaa gtggtacaag
     5161 agcacaatta gaaaagcaat taaaagaatt aaaaatccca atgaatatta gatggtcatg
     5221 ttataactct gaagctatat taagagcagt tgtagataat tttggcattg cagttatatc
     5281 cgaattactt attgaggatt atttaaaaaa gcatcttttg tgggcttgtg atattgaagg
     5341 tattaactta catagaacat tcgatattgc atatcataga agcaagtttt ttacagaaaa
     5401 tatttcagca ttttttgata tttcagtaga atatgggaaa aaacaagcga gaaagaagag
     5461 tagaagtgta aacagtttat aatatatcta aataaaaata agagtatatc aatatactct
     5521 tatttttatt tagatatatt agcctatatt aacgccaaaa tttttacaca atcctgcaag
     5581 tccatcctca aatccagaac ctaaagcatt aaatttccac tctccattat gtctatatag
     5641 ttctgccacg actattgctg tttctatgct aaaatcttca cccaattcat atttaataag
     5701 ttcctcgtta gtttcttcat tatatattct tatatatgaa ttttcaactt gtccgaaatt
     5761 ttgtcttctt gtaattgctt catttatagt aacactaaat gatattttag caatattttg
     5821 aggtatttta gataagtcta catttatttg ttcatcatca ccatcaccaa cacctgttct
     5881 attatcaccc atatattcaa cagctccaga agcgtgtttt aaattattat agaatataaa
     5941 gtctccatca ttcgttactt ttccatcagt tccagttaaa aatgcagagg catctaaatc
     6001 gaaatcaaat ccaccatcat atttattaat atcccatcct agtcctacta ttactttttt
     6061 tagaccaggg ttactttttg ttaaacttac tttttgtcct tttgctaaag ttatacccat
     6121 ttaaaccaac tcctcattat aaatttttta ttgtaattat tacttatatg tattaattaa
     6181 ttcacgaagc tcttcatttt gaccaattcc taaagcatta aatttccatt cttcatttct
     6241 acgataaatt tctcctgcca aaactgttac tttaccagaa aagtcttctt gaagattata
     6301 tttaaataat tcttgcttgg aatttgaatc aattaatctt ataaaagagt tgttcatttg
     6361 accaaaatct tggtctcttt ttttagcttt gtatatagaa actccaaaaa ctattttatg
     6421 tacatattca ggtatgtctt ctaaattaac aattatttgt tcatcatcac cttcaccttt
     6481 tccagttaag ttatctccag tgtgtataat agctttagaa gggtgtttaa gatttccaaa
     6541 ataaaccaag tcacctctaa cattcatttt atcttggtta gtcaatgcta ttgcaaatgc
     6601 atctaggtca taattttttt tacccaattt attcatatcc catcctaagc ctactactag
     6661 attttttaca ggattattat cttttactaa gctaactctt tgtccttttt gtaaactaac
     6721 actcatttta agttctcctt tttatatttt tagttttatt gatggtatca agtataagaa
     6781 ccatattagc tatattatat acgtatttag gctaagttgg ctatatttat aaattattta
     6841 ttatataact acatgaatat tttataattt aaacatattg acattatatg atttaaatta
     6901 ttttgtagtt ttataatatt taaatttgta tttgattgtt ataaagtaaa gtaagtaata
     6961 aagttagact caataattct aaaatttcta agcacactat ctaaatgaga acgtatttgg
     7021 aaatacttgt ataaaacaat aattgataca tttaattttt atcttaaaaa taacaattat
     7081 aatatataaa tagaaagtat aatatataaa tagcaagtat aatataacat ataaaaagtt
     7141 gaatttgtat atgttaatta tacttgctat ttattgttag ttattctatt tttttaaatc
     7201 cattacctaa agcttcatgt atatctgtaa ttataataaa agcattaggg tcgacatttt
     7261 ttactaattt ttttaattcg acaacttctt tcttttcaac tactactaaa agtacatttt
     7321 tattttctcc agtatatgca ccttctcctt ttagaatagt tgcacctcta tctaaatctt
     7381 ccattatttt tttacttata tcttttgaaa ggtcagatat aatagtgaat gatttggaat
     7441 aattaaatcc ttcaataagc aagtcactaa cttttactaa aatgtataaa gccacagttg
     7501 aataaagtgc aatttcaacc tgtctattta ctatacctga taaaacaact acaacgcagt
     7561 ctacaatacc cattaaaaca ggtatactaa atgtaggaaa atatttattt agtaggagtg
     7621 ccattaaatc agttccacct gtagagccat ttattctaaa aataagtcct tgaccaactc
     7681 ccattaaaat agaaccagat ataattgcaa gatacatatc atttgttata tctaatgtag
     7741 ccatatttgc agtaagctta agtgctatgg taagaaaaat tattcctaaa accgttttaa
     7801 aacaatcttt ttttgaaagt attttgtaag caaatatgaa taatggaaca tttaaaagta
     7861 aattgacaat ccaaagttct acaccgaata atgaatttaa aacaactcca agtcctgtta
     7921 taccaccagc agcgattgca tgtggattaa aaaataaatt aagagaaatg gccattaata
     7981 tacagcctac aattaatatt aaaatttctg aaaaagttat tctatctttt ttcaataaca
     8041 tcactcctta tatatagatt ataatatata ttatatagtt tttccaattt ttaataaaag
     8101 aaataaaaat atattataaa aacaatttga ttggaatttt cagaaaatta atgctataat
     8161 taaaagtaaa tttaaaggga ggttataaaa tggctaaatt aactctagaa catataaaag
     8221 gaaatacata ttatattcca ctccctacaa tagtaggtgt atacgtagat ggtaaagatg
     8281 caattttaat tgacagtgga aataataagg atacagctag acaagtttta agattattag
     8341 aagaacataa tttaagtcct aaattaataa taaacactca ttcaaatgca gaccatatag
     8401 gaggaaatgc atatttgaag aatcaaacaa aatgcaagat tgctacgact aaaattgaag
     8461 gatattttac agaaaatcca atattagaat cagcattttt atatggtggc tatccaagta
     8521 aagcactgaa gaataaattt ttattagcaa aggaaagtga agttgattat attatacctt
     8581 caaatggcaa aattgtagat acagaacttg aagcgatttc tcttccaggt cattattttg
     8641 aaatgattgg tgttagaact ccagataatg ttttgtttgt tgcagatagt cttacaccag
     8701 agaatataat tactaaatat cattttttct tcctactgga tatagattca cagtttaaga
     8761 cactagaaaa attgagaatg ttagaggctg atttctttgt tccaagtcac tctgtcaaaa
     8821 ctacagatat aaaaagtttg attaatatta ataagaagaa gatggaagaa ataatagata
     8881 atattaaaaa ggtttgttat gagccagtta tgattgatga agttattgaa aaaatgtgtg
     8941 atttgtacaa tgtcaagctt gatgctaatc aatatgtgtt ggttggaagt acaatacgtt
     9001 cttatataac ttatttatat gagaataata tggttgaata tatatttgat ggtggtaaga
     9061 tgatgataaa agctgtgtaa atagtaataa ttttcaaatt atatgatgaa attgttttag
     9121 tatgactgtt atttgaaaag taacataaaa aaatttatat aaataagcta agatgattga
     9181 tataaagtta ctgataattt taaggtaata tatacgttac tataatatta tgttattatt
     9241 tgtaaataag gggataattt ataagcaaat taaagacctt aatttacagg aggaatagtg
     9301 tatattatga ataaaaaatt caaaaaattc agtaaaaaac taagtatatt aattttagtt
     9361 ttgactatgt ttctttttaa taatttaact gcaaatgcac agattaaaga agattcaata
     9421 ataggaattg atagatatga aacagcagga cttatagcag atagacaaac ttatgatact
     9481 gtaatattag ttaatggaga taaaaattta tctgatgggc tttcatcaag tggattagct
     9541 ggagctatta acgcaccaat acttttgact aaaaaaagtg aaataccaaa agctacttta
     9601 aatagactag ataataagag tttaaatgtt gtaaaaaaag tatatataat tggtggttat
     9661 aatactattg aaaattcagt agaaaaagat ataaaaagaa aaggtattca agtggaaaga
     9721 ataaatggta acaatagaat tgaaactagt tataatgtag ctaaaaaaat aaatgaagta
     9781 agtagtgtaa aagaggtaat gcttacaaat ggatttgtag gagaggctga tgctatgagt
     9841 atagcacctg tagttgcaaa aaataaagga gctataatac ttacagatgg gaaatcagtt
     9901 ccatttaaga ctgaaggttt aaatgtttac gctataggtg gcaaatctgc gataaatgag
     9961 gatttagtaa atgaaactaa agcaactaga atcggaggaa atgatagatt tgagaccaat
    10021 aaaaaggtaa tggaaaagtt ttataatgga gcgacagatt tttatataac aaatggatat
    10081 caattagtag atgcactaac attatctcct ttagccaaag aaaaaccaat cgttttagtt
    10141 gctgatggaa gtaataaagg tattttaaaa ggagctaaaa gtataacaaa agttggaggt
    10201 attgatgcca atatatataa acagtgttta gatatgatta aatataataa tatggatgtg
    10261 actccaaata ttttaaaaca cttaacttca gttgaaggaa atgaagtttc tgaaatactt
    10321 atagaatcag ataatttagg tgtggttgtt gaaaagacga ataccaataa atttgagttt
    10381 gattacgtaa gtgtaactaa tgaaaaaaat tgtacttttt ctgtatataa agaaaattct
    10441 tctaataatg taaaaaatgg aaagctagtt gtaagtgcta agaaaaaaat agataagaaa
    10501 gatagtgatt tgcaagatat gaatggcgac aatatgataa atgcaaataa agataggatg
    10561 gtaaatgtga taaaaattgg agttcccgat aaaatgtatt ctaattttaa agtagatgtt
    10621 gaaagtggtt cagtagaact ttataatatt aaaggaggag ctactgttga tgtcaatgac
    10681 ggaattgcta acattgttga taacaatgtg acaaatcctt tcaatataaa tactaatagt
    10741 gggatttcag gtgtaacagc tgaaactata tcatctgaga taaaatttag agcaaacaat
    10801 ggaattgtaa gtgtaacagc tacaaatata aatggcaata taagtttatt tggaagcgat
    10861 aataaaggta cctttgatgg aatatttaaa ttaaatctga aaaaagaacc atcaaattta
    10921 cacttgaagt tagttggaaa tgggataaat aaattgccag aaggttggtc taaagattat
    10981 attttaggaa atggacaacc aattattgaa gttaaaaata atggcataaa taatatatca
    11041 attggagaat aaaaacatat gaaaataaag tttatatagt tttaaaaatt atagaatttt
    11101 aaaatattta tatatattct ttaaataaaa aattattttt taagtgataa ttaagtattt
    11161 atcgataagg ctatggttgt aaaaatcata gccttatttt tatattataa ataaattaga
    11221 gtatttatca attcatagta gataaaattg aaattaaata taacaacaaa aaatgaaaat
    11281 tacaattaat ttaatgttaa agactcaatt atttggatgt ggaatggata gtacaagtag
    11341 tatataaaaa gtattgactg gacaaataac tatattttat tttaaatata taatataatg
    11401 taataaaata tatcaaatgt agaaggtgat aacctatgaa aaaaataaca ataaatgata
    11461 ttgctaactt ggcaggagta tctaaaagta ctgtatcaag atatctaaat aataaagata
    11521 tcagtgattc tactaaagag aaaataaaga caataataga tgaatatgga tatgagccga
    11581 atgcatttgc tcaaagtttg agagctaaaa aaacatactt tattggtatt ataacaccgt
    11641 gtttagattc ttttgtaaaa tctaaaatta tgatggcaat agatgaagaa ctcaaagaat
    11701 taaaatatac atcactaatt attaatacta atagaaaaat aaggtcagaa atagatagta
    11761 tatcaaagtt agctagtcta aaagttgatg gaataatact aattggtacc gaaataacta
    11821 aagaacataa gaatgaaatt gaaaagttag atataccaat ggttgtagtt ggacaaaaag
    11881 ttgatggtat caatagcata gtgaatgatg attatggagc aggatataaa atgggtcaat
    11941 atattgctaa taaaggttac aaaaacattg tatatttagg tgttgatgaa agtgatatat
    12001 cagttggttt aaatagaaaa aatggagtac ttaatgggtt aaaagataaa gggtatgatg
    12061 caaaagtatt ttatactgac tttgaccaag aaacgtctat acaaagaagt ggagaaatgc
    12121 tagaaagtga aaatccagat ataataatat gtgcaactga taatatagca atagcaacaa
    12181 tgaaagaaat caataagaga ggtaaaaata taccacaaga tatatctgta gctggatttg
    12241 ggggatatga tgtattgtct attataagcc ctaaacttac aacaataaag tttgaaaata
    12301 aaaatgcagg taaagtagca gcaaatacga tagtgaactt aatacaagaa agaaaagaac
    12361 cactattaaa ggagattaaa tttgaattaa tagaaggtga aagtacaata aataaaaatt
    12421 aagatacaca ttacatatat tagtaatgtg tattttttta ctaaaaaaac tataattcta
    12481 aaaattagga aaactttttt gaaagaaata ttgcatctga aataaattta tgctaatctt
    12541 taattaaacc ggtttcgaaa ccggtttaat taaaccgaaa gaatagcaca atgagtgcaa
    12601 aaataactga ttgtaacaaa gtatcaaagt aattattaga gtacatagga aataaacaaa
    12661 atataaaagg tgtagcacat tgtgtaacaa gattaagaat tgttttagat agtaataata
    12721 aagcaaatat aaaattacaa aagaaaagga ataaaaatat gtacaaagag cctaaatata
    12781 gaaccatatt agaatcgata aaagaagaat taaataaatt aagaaatata tctttaaatg
    12841 ataaatataa accactattt catatacatc cacaacatgg actacttaat gatccaaatg
    12901 gattagcata ttataatggt aagtaccatg tattttatca atggtatcca tatgatgcaa
    12961 ctcatggaat gaagcattgg gcttatgtaa gttctgatga ttttgtaaac tggaatagag
    13021 aagatgtagc attaatacca atcgaaagtt atgaatctca tggagcatat tcaggtaatg
    13081 cgatagaggt agatggaaaa ttgcatatgt attatacagg gaatataaaa tatagtgcag
    13141 aagatagata tgcatatcaa aacttagcta taatgaacaa ggatggtaaa ataactaaat
    13201 atgaaaataa tccaatagta agtgaaatac caaaaggata tacaggacat gtaagagacc
    13261 caaaagtatt taaaagaaag gataaatact ttatgttact tggagcacaa acaagtgata
    13321 aaaaaggagt tataatagta tatgaatcta aaaactctat agattggaat tttaaaggag
    13381 aattaaatgt aaaaaatata gatgaagatt ttggttacat gtgggaatgt ccagattata
    13441 ttaatataga tgaaaaagat atattaatat tttcgccaca aggagtagaa cctaaaggat
    13501 ttgattatca aaatatatat aatgtagtat atgctattgg taatatggat ttagataatt
    13561 taacatttga aatagataca atgaaagaac tggaaaaagg atttgatttc tatgctccac
    13621 aaacatttat aaaggactca caaataatac tatttgcatg ggctggaatg ggagaagttt
    13681 tataccctac agataaaaac aaatgggcac attgtctaac tgttccaaga aaattaaata
    13741 taaaaaataa caagctattg caaatgcctg tagatgaatt aattaaatta agatatgatg
    13801 aaacaagtgg gcaaaatact ataaagaata atatcaatat tatagaaaat gatgaaaatg
    13861 tatatgagct taatattaat ataaaaaata ttgacagcaa taaatttggg ctggaattat
    13921 tttcatcaca agatgaaggt gtaaaattag aatttaacaa attaggaaac attgtaactt
    13981 tagacagaag caactttaaa aaagttttcg gtgttgaata tggaacaaat agaaaagaat
    14041 atataaatat tgatgaaaat actaatatca aagtactagc ggatagaagt gttttagaaa
    14101 tttttataaa tgatggagaa gtagtattta ctagtagaat atttgctaaa gaaaattcta
    14161 atcaaataag ggtttatagt gataaaatag tagagtatga gtatactaaa tttaaattaa
    14221 agcaaggtat tgaattataa aaacacatta taaggggtag aaaagatgaa aaaagtaatt
    14281 agtataggag aagctttaat agactttata cctaatcaaa atggttgtaa attaaaaaat
    14341 gtatcagaat ttagaagagt agcaggagga gctccagcaa atgttagtgt tgttgtagca
    14401 aagcttggag gaaaatcaag ttttatatct aagttaggaa aagatgcatt tggtgattat
    14461 ataatagatg tattaaatga agtaaatgta aatactgatt atgtattgag aacaagtaaa
    14521 gcaaatacag gacttgcttt tgtatcatta aaagaagatg gtaatcgtga tttctcattt
    14581 tatagaaatc caagtgcaga tatgctttta gaagctgatg aggttaaaaa agagtggttt
    14641 aataattgtc atatcttaca tttttgttca gtagatttaa tagatagtcc aatgaaatta
    14701 gcacataaaa aagcaataga atacgcactt gaaagtaata gtataataag ttttgaccct
    14761 aatataagat tacctttatg ggatagtgag caatcttgta aaaaggcaat atcagaattt
    14821 ttaccttttg ctcatattgt taagatatct gatgaagaat tagaatttat gacaggagaa
    14881 aatgatatag aaaaatcatt acataaactc tttgtgggga atgttgaact agtattatat
    14941 acaaaaggaa aagatggagt agatgcatac actaaaaaag taaaaggtat gtgtaaaggg
    15001 gttaaagtaa atgcaataga tacaaccgga gcaggagatt cttatatagg ttcattttta
    15061 tacaccttat tgtataaaca aataacattg aatgatataa aagaaatgaa acaagaaacg
    15121 ttaaatgagt atttagaatt ttctaattat tatgcagctc gtagtacaac tattaaaggt
    15181 gcgataagtt catatgcaac aaaggaagag ataactgaat ttataaaaaa tttatagtag
    15241 gaatgagtaa taggttttga aggaagtaac atgtaaagag tttttaagga tgtttactat
    15301 gaaaataatt aacatataaa aagcaataaa gaggctgtct caaaatagat ataagcctct
    15361 ttatcattgt tgaaatgata aaaaatattt taattattgt tactttttag aagaatatat
    15421 atcttatttt aaataagcaa attatgtaag atttatttat attatcaata ctttacttgg
    15481 agtattatgt ctttagcagt tatctacttc aattgaatta tctaaaatat aataaatttg
    15541 ttttgcaaat tgacgagaat gtttttcttt ttctttattc ataaaattag gtttacctaa
    15601 atcacgttca acaagcattc ctatatattt taactgtgaa tgcttacagt agcgcttcat
    15661 accattttcc caaatatcag cacccttttc aggtctatat ccacatgttg taataagtgc
    15721 tactggtttt cctgcccata gagcggggcc tttctcatta ccatagtact tattcattcc
    15781 atacaccaat ctgtcaagta gtgctttcat tggagaagtg cagtaccaag aataaattgg
    15841 agtagcaaaa acaattaaat cacacataag aattttcttg aaaattttct gaacatcatc
    15901 tttacaataa catccaaaat caaataagtt atcctgacat tttttacaag caatacatgg
    15961 ctctatttta caatcatata accatattac ttcaatattt gaattataat tttctaattc
    16021 ctcaataaaa ggttttaaaa tagcagcagt attacctttt ttcttaggac ttcccataag
    16081 aatgcatatt tccatattga tacctccaca gtattttaaa atagttttta gtgcttaata
    16141 tagttttagg atactatgag aatacgttaa caacaataca ctttgtatat ttttaataaa
    16201 attgaaacaa attgttttat caataaaagt tggaatgtat ctataattaa aaatatttta
    16261 ggtatgtttt tacatatatg tgtgtagtta actatggtaa tttattgtaa aattatctta
    16321 taggattata attgcttaat ttatgattat atttaaaatc ttggaggtaa aaaatatgta
    16381 ctcaaatgaa gactataaat ttatagaaga aaatgcacca tgttatatat atgatgagac
    16441 taatataatt gaaaaatgta aggaattgaa aaataatatt ttaggttttg attttttata
    16501 ttctataaaa acaaatccat ataacaaaat tgtaaaaagc attgcagagc aaggttttgg
    16561 agcagatgct tcttcaacta atgaagttat gatttcatca gaatgtggag tttctaaata
    16621 tgatatttat tattcatcac caggcaaaac agagattgat atagaaactg ttttagataa
    16681 atgcataatt attgcagata gtattacaga aatagaactt ataaataaga tatctaaaaa
    16741 gcataatcag aaagttaaaa taggaataag aataaatcct gattttacta tggatgatga
    16801 tagtgctaaa tcaagcaaat ttggtataga tatagaagat atgagcagtg tttttaagat
    16861 gttaagtatg tgtgattatg tatctgtagt tgggattcat atacatatta aatctcaggt
    16921 attggatttt gaaaagttag gacattatta tgaaaaatgt ttttttatag ctttaaattt
    16981 aaataaaagt aagtatatta atattgaatt tataaatttt ggtagtggac ttggagtagc
    17041 ttatgataat cttagagaaa atccacttaa tcttaaagag ctttcaaaat taactcaaaa
    17101 gattgttttt actaattcaa acacactgaa atcaagactt cttattgaat caggacgttt
    17161 catagtttgt aaatcaggta attattatgt taaaattgta gatattaaag tatcaagagg
    17221 gaaaaaatat cttattgtaa aaaatggact taatggattt atgagaccta ttatttctaa
    17281 tttaattgaa aatattactt gtggagtaga attagaaggt caagagccat tatatacatc
    17341 aaatcatgcc ttttctcttt cagtattaaa taatttaaaa gaagaagagt atgtaaatgt
    17401 tgttgggaat ctctgtacat cattagatgt aattgcaaat aatgttaaat tgaaaaaagc
    17461 aaatatagga gatttgattt caattagtaa tgctggaagt tactcctatt ctttatcacc
    17521 actattattt tcgagttatg aatttcctaa acaatattat aaaagaaaag atggatatat
    17581 tgagtaaaca ataggataaa ttaataaact tagtattatt gaaaaaattt aaaatgcagg
    17641 gggtgtaatt ctatatggaa ttgtggaaaa gaaatttatt tgtatgttgg ataggtatgt
    17701 tttttagttc tatagggatg agccaaatag cacctatact tccattatat attaaacagt
    17761 tgggggtaac tgatgttagt ttaatacaac agtattcagg tataattttt ggatgtacct
    17821 ttgtagtagc agcattcttt tcgccaattt gagggaaagc tgctgataaa tatggaagaa
    17881 aatcaatgct tttacgtgca agtattggga tgggaatagt tatatttacc atggcgtttg
    17941 ttcaaaatgt ttatcagtta ttaggattaa gaatacttca aggagttttt acgggttatg
    18001 ctactgcatg tactactctt attgcaacac agacagataa aaatcattca ggatgggctt
    18061 tgggtactct tgcaactgca tcaactactg gctcattaat tggtccaaca gtaggaggat
    18121 atatagaatc aatcttgggt cttaaaagta catttattat aacaggagga ttgttgtttg
    18181 tatcatttat catctctatt ttatttgtag tagataattt taaacctaaa gaaataaaag
    18241 aatcaatttc tatacctaaa gaacaattaa atatattgcc aaataaattt ttaattgcat
    18301 ctatttttgt aaccacattc attactcaac tagctttata ctctatagaa ccaattgtaa
    18361 ccatatatat atcacaactt acaaattttg catcaaatgt agctttaata gctggattga
    18421 cattttctgc atcaggatta gcaaatttat tagcagctca aaaacttgga aaaatgtcag
    18481 ataaaatagg tcctcaaaaa gtattgttaa tttcactttt atgggctggg attatattta
    18541 taccacaagc ttttgtaaaa actgcatggc aactgatgtt gttgagattt ttattagggt
    18601 tgtcagttgc agggcttaat ccttctgtta acagcttgct taaaaaaatt gcaccagaag
    18661 aatatgtggg aaaaatattt ggatataatg catctgccca gtatataggt tgttcatcag
    18721 gagcattttt agggggacaa atatcagcac atttaggtat tagaacagta tttttttcaa
    18781 caagtttatt gttgtttatg aatgcacttt ggatgtataa atttactgga ttgtttatta
    18841 aagataagac taaataatca tactatttac atttctaatt ttatgatgta aaataaatat
    18901 tttttatgta atttatcata gaattatata aaaaataact ctatggtaaa aattggaaaa
    18961 tattattatc gacaaaatct ttgacttaaa aactatattg cgatagtatt aatttaataa
    19021 aagtttgaga ggggcgtaag gttatgacaa tttcaaaaat accacaagca gaattaaaag
    19081 ttatgaagtt tatttgggaa aaaaatgata cagtaacatc aaaagaaact attgaagcta
    19141 tggaacgaaa atatggttgg aaacaaacaa ctacattgac acttttatca agacttgtta
    19201 aaaaacattt tttaagtgca gaaaaaatag atagatacac tcattataca atagttgttg
    19261 gacataagga gtatttaagt gttgagacaa aagatttttt aagcaatata cacgacaatt
    19321 ctttacagag tttaatttca gcactacatg atgatgaagt tgttgataaa gatatgttgg
    19381 atttatttga aagtcatttt aaaaatttag aagaagagta attatatcag gataaaactc
    19441 taagaattta attaatctat ggctgaaaac cataaatggt attatttaat cagctactca
    19501 ctattcataa agtaatatta aataggttct ctaattagta aaatacctaa aataattgga
    19561 atagggagta tcaagttaaa tgataccact tatttaaata agaaatgatt tttaaaattt
//
LOCUS       DAELSY010000070        19341 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.070, whole genome shotgun sequence.
ACCESSION   DAELSY010000070 DAELSY010000000
VERSION     DAELSY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19341
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.070"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1187)
                     /locus_tag="KPG09_002177"
     CDS             complement(<1..1187)
                     /locus_tag="KPG09_002177"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBG0966786.1"
                     /translation="MVFKNNKGVNYMFNTIEEAIEDIKNGKMVIVVDDESRENEGDLL
                     MAAEMATPESINFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGA
                     DTLTGISAYERAETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLA
                     RLAGFKEIGLICEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKV
                     SSAFLPTKYGNFEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKC
                     DCGLQLESSMKKIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGF
                     EEDMRDFYMAAQILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKL
                     YLKT"
     gene            complement(1177..1842)
                     /locus_tag="KPG09_002178"
     CDS             complement(1177..1842)
                     /locus_tag="KPG09_002178"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBG0966787.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(1876..2979)
                     /gene="ribD"
                     /locus_tag="KPG09_002179"
     CDS             complement(1876..2979)
                     /gene="ribD"
                     /locus_tag="KPG09_002179"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBG0966788.1"
                     /translation="MNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNIIGK
                     GYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKRVVI
                     STLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKSAVS
                     LDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQEKVS
                     HPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSKNNL
                     VDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPTFVS
                     GEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(3111..3232)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3421..5904
                     /gene="recQ"
                     /locus_tag="KPG09_002180"
     CDS             3421..5904
                     /gene="recQ"
                     /locus_tag="KPG09_002180"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBG0966789.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDLKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GANILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVMLDSYLENLKNNIIAE"
     regulatory      5992..6103
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6245..7546
                     /gene="thiC"
                     /locus_tag="KPG09_002181"
     CDS             6245..7546
                     /gene="thiC"
                     /locus_tag="KPG09_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBG0966790.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            7600..7794
                     /gene="thiS"
                     /locus_tag="KPG09_002182"
     CDS             7600..7794
                     /gene="thiS"
                     /locus_tag="KPG09_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBG0966791.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            7787..8590
                     /gene="thiF"
                     /locus_tag="KPG09_002183"
     CDS             7787..8590
                     /gene="thiF"
                     /locus_tag="KPG09_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBG0966792.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            8653..9423
                     /locus_tag="KPG09_002184"
     CDS             8653..9423
                     /locus_tag="KPG09_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBG0966793.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            9440..10543
                     /gene="thiH"
                     /locus_tag="KPG09_002185"
     CDS             9440..10543
                     /gene="thiH"
                     /locus_tag="KPG09_002185"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBG0966794.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYEFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            10544..11233
                     /locus_tag="KPG09_002186"
     CDS             10544..11233
                     /locus_tag="KPG09_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG0966795.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            11421..12815
                     /gene="yfcC"
                     /locus_tag="KPG09_002187"
     CDS             11421..12815
                     /gene="yfcC"
                     /locus_tag="KPG09_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBG0966796.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            13332..14699
                     /locus_tag="KPG09_002188"
     CDS             13332..14699
                     /locus_tag="KPG09_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBG0966797.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            14724..15332
                     /locus_tag="KPG09_002189"
     CDS             14724..15332
                     /locus_tag="KPG09_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBG0966798.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            15380..15751
                     /locus_tag="KPG09_002190"
     CDS             15380..15751
                     /locus_tag="KPG09_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBG0966799.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            15930..16268
                     /locus_tag="KPG09_002191"
     CDS             15930..16268
                     /locus_tag="KPG09_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBG0966800.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            16735..17238
                     /locus_tag="KPG09_002192"
     CDS             16735..17238
                     /locus_tag="KPG09_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBG0966801.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            17285..18244
                     /gene="moaA"
                     /locus_tag="KPG09_002193"
     CDS             17285..18244
                     /gene="moaA"
                     /locus_tag="KPG09_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBG0966802.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            18248..18718
                     /gene="moaC"
                     /locus_tag="KPG09_002194"
     CDS             18248..18718
                     /gene="moaC"
                     /locus_tag="KPG09_002194"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBG0966803.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDYMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            18754..19185
                     /locus_tag="KPG09_002195"
     CDS             18754..19185
                     /locus_tag="KPG09_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBG0966804.1"
                     /translation="MAKVVSINISEKKGVIKVPVPCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         7555 a         2064 c         3359 g         6363 t
ORIGIN      
        1 gtttttaaat atagcttatt aaagtcatta atttcttcat taatagggat tctattttcg
       61 atttttattc catatttctc taattgattt attttatccg ggttattact taaaagattt
      121 atacttttta tgtttaaatt ttttaatatt tgagcagcca tatagaaatc tctcatatct
      181 tcttcaaatc caagcattaa atttgcttca acagtatcgt aaccttcttc ttgaagttta
      241 taagccttaa ttttatttaa aaggccaata cctctacctt cttgcttcat ataaattaaa
      301 actccacttc cattttccac aattttcttc atagaagatt ctagctgtaa tccacaatca
      361 cattttagag agtggaacac atctcctgtt aaacattcag aatgaagtct aactagagta
      421 ttttctatat ttatatttcc ataagtaagg gcaatgtgtt cttcatttga ataagtatct
      481 ctatatccta tgatttcaaa atttccatat tttgttggaa ggaaagcact agatactttt
      541 tctatagtag tttcatttat tttgcggtat tctataaggt cttttatagt gatgattttt
      601 aaattatgct tttctttgaa aatcattaaa tcatcaactc ttgccattgt accatcatct
      661 ttcattattt cacagataag accaatctct ttaaaacctg caagtctagc aaggtctact
      721 gaggcttctg tatgtccatc cctgacaaga actccaccag tttttgctat taaaggaaat
      781 atgtgaccag gcgttttgaa atctttactt gtagaatttt catcacagaa caattttaca
      841 gtttcagctc tttcatatgc agatatacca gttagagtat ctgctccatc tatagtaaca
      901 gtaaaggctg tttgaaatgt atctgtattt tctctaacca taagaggtat attaagactt
      961 ttaaattttt tttcagtggc aggtagacaa attagacctc taccatatgt agccataaaa
     1021 tttattgatt caggagtagc catttcagct gccatcaata aatctccttc attctctcta
     1081 ctttcatcat caactacgat taccattttc ccatttttta tatcttcaat agcttcttca
     1141 atagtattaa acatgtaatt cactccttta ttatttttaa aaaccatttt taaataaaaa
     1201 atctttatct atatttgatt tatggttact actattagaa tttaattcct tgtagttatt
     1261 agccataaaa ttattaacat actttccaat tacatcgctt tctaaattaa caaaatctcc
     1321 aacattcttt gtcaagagaa tagtgttaat tttagtatga ggtataatag aaaccttaaa
     1381 tatttcatca tctaaatatg atatagttaa acttacacca tcaatagcaa cagaaccttt
     1441 gagtatcatt gaagataaaa gttttttatc aggtcgtata cttactaaaa ctgcattttc
     1501 atctttttca aatttgcaga tagttccttt accatcaaca tgacctgtta ctatatgacc
     1561 accaaatctt gaagaaagac tcatagctct ttcaagattt accttatcat ttgcatttaa
     1621 taaacctaaa tttgtggaac gaattgtctc catcataaca tcggctgtaa attcattttt
     1681 tccgaggttt gaaacagtta gacaaactcc atttacggct atactatctc caagatttgt
     1741 gccatctaat attttatttg atagtatggt tacttttcca ctcttaccat tagcagtaat
     1801 ttttctaagt attccaactt cttctactat gccagtaaac atcctaacct cctatatgaa
     1861 tgtattaaca aaaatttaat ttaaaacatc tgattctata agaatatcgc catctatcaa
     1921 agttacatta ttgattgtta actgtgttga atctgacaaa tgatttattc cttctccaga
     1981 aacgaatgta ggtgcatctt taccacctat aatcttgggt gatagaaata atttgacttt
     2041 atcaactaac ttatttctaa agagactgtc attgagagta gcaccacctt caactagaac
     2101 agaatcaatg tttaactctc ctaatttata cattacaaat tccaaatcta ctaagttgtt
     2161 ttttgaagga gaaattattg tttcaacatt attttctttt aacatagata attttatact
     2221 ttctttgcaa catgtgaaaa ctatagtttt actggtttta tctttaacca aattactatt
     2281 aagtggcaac tttaggtgtg tatctattac tattcttgta ggatgagaca ctttttcttg
     2341 attgagcctg caagttagta aaggattatc gttcaaaact gtatttatgc caaccattat
     2401 actttggtat ttatttctgt atttatgagt tagatacctt gatgattcat tagatatcca
     2461 ttttgattct agtgattttg tagcaatttt accatctaga cttactgctg attttacaat
     2521 acaaagaggt cttttgtttt ttatatgata aaaaaatgcc tcatttaaat ctcttgcttc
     2581 atcttctaat attcccacag taacatcaat attgttatct tttaattttt ttactccatt
     2641 accacaaaca agtggatttg ggtctaatgt agatattaca actctcttaa tcttattttg
     2701 tataatttta tctacacaag gaggcgtttt cccataatga gaacatggtt ctaagtttac
     2761 ataaagagta gagtctttta gactttgttt agcactgttt atagcattta cttcagcatg
     2821 attagagcca aatttctcat gatatccttt gcctattata ttactatctt taacaattac
     2881 acacccaact aatggatttg gatttacaaa gccttctcca tttttagcca attcaatagc
     2941 ttttttcata taataaatat ctttttcttt ttgattcaca atatgtacac ctcctgttta
     3001 attggataat ttttaaacaa aaaaccctga gaattaactc agggtttaga ataatctaca
     3061 ataaatgttt aataaaagta gaagaaaatt gctctacaaa aacactcatt aaatatcttc
     3121 ttccatccag actttactgt cggctttgga atttaaccaa atcaaccaag ttttaccttg
     3181 gctcgcgggc tataccgccg attgggaatt tcaccctacc ccgaagatta agtattaaat
     3241 ttatatttag aatgtatcat aaattatttt actttgcaaa ctattttttt aaaaaatata
     3301 tgttaatata taatatagaa tgtctataaa ctttgaaata ccacgattgt gaatataaaa
     3361 ataaaaattt gaacataatc aggaaagaag tctaattaat ttaaattagg agagtaatat
     3421 atgaataaaa aacttttaga aatactatca aaatactatg gatatacaag ttttagaaaa
     3481 ggtcaggaat ctataataaa ttctatctta agcgaaaaag atgtgttagc tattatgcca
     3541 acaggagggg ggaaatcaat atgttatcaa attccagctt tgattttaga tggaatgaca
     3601 atagtaattt ctccacttat atcacttatg aaagaccaag tagatgcctt aaagactatg
     3661 ggaattagtg ctgcttttat aaatagttca ttaagcagca aagaatttaa tgaaatatta
     3721 aataatatta gaaaaaataa ttataaaata ctctatattg ctccagagag attagatgca
     3781 caagagttta ttgagctaat aaataataac aatgtaagtc aagttgcgat agatgaagca
     3841 cattgtgtat ctcaatgggg gcatgatttt aggttgagtt atagaaggat ttctgatttt
     3901 ataaaaaatt tgcctaaaag acctatagtt acagcattta ctgcaacagc ttcagaagag
     3961 gtaagaactg atataataaa tttactgtgt ttagaaaatc cagattatta tataactgga
     4021 tttgatagag agaatttatc aataaatata gtaaaatcat ctagtaaaaa taagtatatt
     4081 ttagattata ttcaaaatca taaaaatgag tctggaatta tatatgttgc aactagaaag
     4141 gaagttgaaa atatatataa tggtttatct aagagaaata tatctgtatc aaaatatcat
     4201 gcaggtttat ctaaaaatga aagaagtaaa aatcaagaag attttataaa tgataatgtg
     4261 gatataatgg tagcaacaaa tgcatttgga atgggtattg ataaacctaa tataagatgg
     4321 gttatacatt acaatatgcc tcaaagtata gaaaactact atcaagaaat aggtagagct
     4381 ggtagggatg gagaaaaaag tgagtgtgta cttttatttt ctccaggaga tgtacatata
     4441 caaaaatatt taattgatat tggagttgaa aatcctgaaa gaaaattatt tcagcataag
     4501 aagcttcaat acatgattga cttagtttac agtaattcat gctatagaaa gacaatattg
     4561 agttattttg gagaaaatta tatagacaat tgcaataatt gtagcaattg tttagtagag
     4621 ggtgaaatag tagataaaac tatagatgca caaaaagtca tttcatgtgt agctagaatg
     4681 aaaagaagtt atggtgttac tactattgta gatgtactta gaggttctaa gaataaaaag
     4741 attttacagc ttggattaaa tactttgtct acatataaca ttatgagaga ttattctagt
     4801 gaagacttaa agaattttgt aaatacattg gtttctcatg gttttttaga tttagttgag
     4861 actctaggca atggaaatag aggtagtttt cctactataa gattaaatga gatgtctatg
     4921 aaagttttaa aaggtgaagt gaaagtagag tttaaagaaa ttgttatgac aaaatcattg
     4981 gaagtagagg atgagttata taatgcttta agagaactta gacattcaat tgcaagtgaa
     5041 gaaaggatag ctccatatat ggtatttgga gatggtacac ttagagccat gtctagtagt
     5101 tatccaatta ataaagaaga aatgctagat atctctggag taggagaaat taaatatcaa
     5161 aaatatggta gagattttga agcagtaatt aaagaatatg tcgaaaaaaa taatataaat
     5221 aaaaaaatag atgaaaaaaa tgtcgattta aagaacgtta atagtgattt cttagaggtt
     5281 aatacagata aagttttata tgaaaaactg ttagtcatta gagaagaata tgcaacaaag
     5341 gaaaaattac ctgcatatat gattttagcc aataaggctt tgaaagaaat aagtggaaga
     5401 tatccacttg atgaagaaca attaaaagat atttctggca taggtggagt aaaatttgaa
     5461 aaatatggag ctaatatttt ggcagaggtt aataaatatg taaaagaaaa taatattaat
     5521 gttatttggg agaaaaaggg caaaaggaag ttaatattag atggagaaag tagaaaaaat
     5581 aatgaaatag cattagattt gctaaatcaa ggaaaagata tacaaagtat cagtcaagaa
     5641 atagaagttt cattgtcaac cattattgga tatgtgcatg actatgtaaa ggatggacat
     5701 agtatatcat ttgaattagg actagaaaat ttttataatg aagataaaaa gaagatgatt
     5761 ttagaagttt gcgaaaaagt tggctatgat aatttaaata acataaaaag aaaacttcca
     5821 gattcaataa agtatgaaaa tataagagct gtgatgcttg actcttatct tgaaaactta
     5881 aaaaataata ttatagcaga gtaattttta acttaataaa aataaaattt gtattattgt
     5941 gttgaactaa taattttgct gtgttatact gaatttgtaa aaataatgaa aaattagagc
     6001 taggggcgct agaattggct agctgagaga taaagccgta acttacaaac cctaataacc
     6061 tgatcaagat aatgcttgcg aagggaagct ttttaatata attttattat ataaataggc
     6121 aaattattca atgttaggag aaatttgtct aagtgtatat acgattataa gagtgctttc
     6181 caaacgagga aaagcacttt ttttattttc aaaaactata actttaatct tggaggattg
     6241 taatatgaac tatacaacac aaatggatgc tgctagaaaa ggcataataa ctaaagaaat
     6301 ggaaatcgtt tctcaaaaag aacaggtaga tgtaaatgaa ttaagagaat taatagcaaa
     6361 tggacaagtt gttataccag ctaataaaaa tcataaatct ttaagtgcag aaggtgtagg
     6421 taaaaattta agaacaaaaa taaatgtaaa tctaggaatt tcaagagatt gtaaggatat
     6481 agaaaaagaa cttgaaaaag ttagagtagc aatagacatg aaagctgaag ctataatgga
     6541 tttaagtaat tatggcaaaa ctagagaatt tagagaaaag gttgttgaaa tgtcacctgc
     6601 aatgataggt tcagtaccta tgtatgatgc agtaggatat ttggaaaaag aattaaaaga
     6661 tataacagaa gaagagtttt taaatgtaat aagacaacat gctattgatg gagtagactt
     6721 tattactata catgcaggtc ttacaagaag tgtttgccaa aaaataaaaa atcatgagag
     6781 attgactcat atagtatctc gtggaggttc tttattgttt gcatggatgg aattaaataa
     6841 taaggaaaat cctatttata ctaattttga taaaatattg gatatatgtg aagaatatga
     6901 tgttacattg agtttaggag atgcttgtag accaggttgt ataaaagatt ctactgatgg
     6961 tgttcaaatt caagaacttg tagtattagg tgaacttact aaaagagcat gggaaagaaa
     7021 tgtacaagta atgatagaag gtcctggaca catggctatt gatgaaatag aagcaaatgt
     7081 agtattagaa aagagattat gccatggagc acctttttat gttcttggac cattggttac
     7141 agatatagca ccaggttatg accatataac aagtgcaata ggaggagctt tagcatgtgc
     7201 taaaggagta gattttttat gttatgtaac tccagcagaa catttaagat tgcctaattt
     7261 agatgatatg aaagagggta taatagctgc aaagatagct gctcatgcag gagatatagc
     7321 taaaaatgtt aaaggtgctc gtgaatggga taataagatg agtaaagcta gagctgattt
     7381 agactggtgt gagatgttta gacttgcaat agaccctgaa aaagctaaga gatacagaga
     7441 tgaatcaact cctactcatg aggatagttg tactatgtgt ggaaaaatgt gctcaatgag
     7501 aacagttaag aaaatattaa acaatgagga acttaatcta atataatttt tggctagtat
     7561 gactggaata aaaagaagct gaaaagagga ggtgatttag tgaaggttaa tgggaaggaa
     7621 attgaatttg aaaaagattt gactgttata gatttattaa ataaatataa tttaaaaagt
     7681 gatagagttg tagttgaagt gaatttagag ataattgaag aatctaacta caatacatat
     7741 gtattaaaag acgaggacat tgtagagctt attagcttta ttggaggtgg ttaaaataaa
     7801 aatatttgta aatgaattat taacagatgt tgaggaaaat acaactgctt ataaagttag
     7861 agatagctat aacaataaat gtgatgtatt ggtgttaaat ggatatccaa ttaaaacaga
     7921 tatgcccctt tgtgaaaatg ataaattaac acttatacaa aaaggagtta agccatcact
     7981 tgatgaattg gaaagcttgt tgattgctag acatactcca aatatacata ataaattaaa
     8041 aaaagggaaa gttgctatat tgggattggg gggacttggt tccaatatag ctatctcttt
     8101 agctagaata ggagtgggag aacttttact tatagattat gatgttgtag aaccatctaa
     8161 tttaaataga caacagtatt ttatagatga tataggaaaa ctaaaaactg atgccatgat
     8221 agaaaatata aaaaaaataa atccatttat aaaattaaat aaacgagata tttttttaaa
     8281 caagaacaat atggatacta ttaaggattc agatttaata attgaggcct ttgatgatgc
     8341 ttcatgtaaa gcacaggtat gtaattatgt attaataaat ttaaaagata aatatttaat
     8401 agcatcatct ggtatggcag gttactatga ttcaaatata ataactacta agaagataaa
     8461 agacaggttt tatatatgtg gggattttgt gaatgaagct aaatttggtg aagggcttat
     8521 ggctccaagg gttgccatct gcgcaaatca tatggcaaat ttggcaacgc aaatacttat
     8581 agatatgtaa atattaaaat tagatataaa aaagattgaa tattaaaaaa atttaacgtg
     8641 gagggaaata atatggataa attggtactt ggtggacatg aatttaatag cagattattg
     8701 gttggaacag gtaaatatgg ttcaaataat atattgcctg aggtaataaa agaaagtgga
     8761 agtgaaataa taacaatggc acttagaaga gtggatttag acaataaaca agaaaacata
     8821 ttaacgtata ttccaaaaga gatgacaata cttccaaata catcaggagc tactaatgca
     8881 gaagaagctg taagaatagc tagaatatct agaaaaatgg gatgtggaga ttttataaaa
     8941 atagaagtaa tatcagatac tagatattta cttcctgata atgaagaaac tataaaagct
     9001 acaaagatac ttgctgatga ggggtttata gttcttccat atatgacacc agacctttat
     9061 gctggaagaa gacttataga agctaatgca gccgcagtta tgccacttgg agctccaatt
     9121 ggttcaaata gaggtcttca aatgaaagaa atgataagaa taatgataga tgagttggat
     9181 ataccaataa tagtagatgc aggtattgga aaaccatctc aagctatgga ggctatggaa
     9241 atgggagctg atgcagtact tgtcaacact gctatagctt cagcaggaga ccctgtacaa
     9301 atggctagag catttaaatt agctgtagaa ggtggaagag aagcatatat tgcaaaaaca
     9361 ggaaatgtgt cagaatttgc aaatgcatct tctcctttaa caggtttttt aggcaattta
     9421 tagaaagtgg tgattggtga tgagttttta tgatgtaata gaaaaatata gggattttga
     9481 ttttgatgga tatttaaata atgttactga taatgatgtt ttaagaagtt tatcaaaaga
     9541 caagcttgaa gattttgata tattaaattt gctttctaaa actgcagtaa aacatttaga
     9601 agatatggca caaaaagcac ataaattaag tgttcaatac tttggtaaaa cagtatgttt
     9661 atatacacca atgtatatag caaattattg tgttaatcaa tgtgtttatt gtagctataa
     9721 tataaagagt ggaataaaaa gaaaaaaatt aactatggat gaaattagag aagagggaga
     9781 agcaatttca aaagaaggtt tcaaacatct acttgttttg actggagaaa gtagttttca
     9841 ctcaagtgta gaatatatag gtgaggctat agaaatatta agagagaaat ttccctctat
     9901 aggtatagaa gtttatccaa tggaagtaga agaatataag tacattgtag ataaaggtgt
     9961 tgaaggatta acagtatatc aagaaactta tgacgaggaa atatataaga gagtacatat
    10021 aaaaggccca aaatcaaatt atgaatttag actagatgct cctgaaagag gggcaaaagc
    10081 aggtatgagg actttatcaa taggtgcact tttaggtcta aatgatttta gaaaagaaac
    10141 tttttttaca atattgcatg ggaaatactt aaaaacgaaa tatcctcata ttgagctttc
    10201 atattcaaca ccaagaatga gaccttttaa agggtgtttt gaggaattgg tagatataag
    10261 tgatactgat ttagttcaag ctatggtatg tatgagatta tttgaccctc atgcagctat
    10321 aaatatatcc actagagaaa atttagaaat gagaagtcat ataatacctt taggagtaac
    10381 taaattgagc gcaggtgtat ctactgatgt aggaggtcat tctcaagatg aacatgatac
    10441 tgctcaattt aagataaatg atgaaagtac tgttaaagat gtggagaaaa tgctaaactc
    10501 aattggttat cagcatgtgt ttaaagattg ggaaagattt taaatgtatc tgataacaaa
    10561 cagaaaatta tgtagtgagg aacgatattt agaagttatt aaagagtcta tattgagtgg
    10621 tgtagaaaat ataataataa gagaaaagga tttggaatat caagagctaa gaaaattata
    10681 tatgaaaata aaaactaaaa tcaattgtat agattttcaa gagcaaatat cagatgaaag
    10741 tttgaaaact aatataaatc aaaaagagtg tagaaataaa ttcaaggtta attttataat
    10801 aaatagtaac attgaatttt ttgaaaaagt ggattgtcaa ggtatccatc ttccttttaa
    10861 attattcttg aatttaattg aaaataagta taattttaat gaaaataaaa tactaggatt
    10921 atctttacat aaagtagagg aagtagatta tttagaaaaa ttaataagaa atcaaaatat
    10981 aaaaatagat tacataacat tatcacatat atatgaaact aaatgtaaag aaggattaaa
    11041 tccaaaagga attgagcttt taaaagaagc taaaaagatt actgatatta aaataattgc
    11101 acttggggga attttaccat ctaatgtcaa agaaacatta aaatactgcg atgattttgc
    11161 tataatgtca actataatga ggtctaaaga tattaaaaaa accatatcaa attataatga
    11221 aaagttgaat tagtaaatat agtatattga atttttaact taaataaaaa taaaaaagtt
    11281 atcttttaaa ttttaaaagg tagctttttt attttatatc tttgctaata aaaaattgta
    11341 aaaagggaaa actttagtca gagagattaa acaatatcct agaaagattt aaaatttagt
    11401 aaagtatgga ggacattata atgactgaca gtaaaaagaa acgatttatt ataccacaca
    11461 cttttacaat aatattttta ctaatagtct tgatggcaat attgacttgg atagtaccaa
    11521 gtggtgaatt tagtagagaa gatgtagaag gtagaatggt agttgtacca gggacttatc
    11581 aaagtgtaga aagtaatcct caaggatttg cagatgtttt taaagcacca atagaaggct
    11641 ttatagattc agctgaagtt gtaggttttg ttttaatagt tggaggtgct tttggtattg
    11701 taaataggac tggagctata gaagcaggaa tagcttcggt tatctctaag tttaagggaa
    11761 aagaacttct tataattcca atatcaatga ttttatttgg acttggaggt acaacatttg
    11821 ggatggcaga agagacatta ccattttata tgatatttgt tccactcatg acatccttgg
    11881 gatatgattc tctaacagca atagcaattg tatttatagg agcagcagca ggagccgccg
    11941 cttcaacaat aaatccattt tcagttggta ttgcacaggc actagctcaa cttgctcctg
    12001 gttcaggagt aacttacaga gtagttattt ttatagcata tatactaata agtatagcat
    12061 ttgttatgta ctatgctaag aaggtaaagg aaaatcctga aagttcaata gtatatgaaa
    12121 tagataaaaa taataaatca atacttaatc aaacttcaac agaaataaaa gaatttactt
    12181 ttaaagaagt atctgttctt gctatatttg gaatagggat ggcaataatg atttggggtg
    12241 ttttaagtca aggatggtac attccagaaa tatcaatgtg ttttgctgca ataggtgtgc
    12301 tttctggatt ggttggtaga ttatctcaaa ttgaaatctc agaaagtttt atagatggag
    12361 caaaagattt agcatctgca gctcttgcta tagctttggc tcgtggaatc gtaataattg
    12421 ctcaaaatgg atttataata gatactatat taaattccgc agcagccttt ctaggtgggc
    12481 ttccaaaggt tatatttgta tatttatcat ttttcttgga agctgcattg gcatttttaa
    12541 ttccatcatc ttcaggtctt gcttcactta cagtacctgt acttgctcct cttggtgatt
    12601 tggttaatat atcggctcaa gtgatagtaa ccgcttatca atttggtgtg ggtcttacaa
    12661 actttataac tccaacttca ggagtactga tgggagcact tgcagtagcc catattccat
    12721 tttcaaaatg ggtaagattt ataatgccta tcttgttgat tttgacagct gtatctctag
    12781 tctttttaac aataggtatt tatatagggt tttaattaag tgtagtttta tttaagatat
    12841 ttagtaatgg tataaaatga taatatgttt tcaataaatt gccactaatt taattagtgg
    12901 taatttattt tttgtattta aaaataaatg tatttacttg tattttgaat taatatctac
    12961 ttagacattg ttttaattgt tatattttca tttaattaag cattgattta tcgtttttta
    13021 tatttcatat aacaatgcaa aaaataattt ttatatagta aatgacaaaa gtttacaata
    13081 aatgtatatt ttcttactca ttccttaaaa ttactcattt agtttaataa ctctcatttc
    13141 atatgtaaag ttggtaaaaa aattaaaatt aaaagtaaaa aactaaataa attaatgtta
    13201 atttgaaata tatatgaagg tttttggaga atacttactt atgataggaa aaaatgttaa
    13261 aataatagtg taatcaaata taaaggactg aaataattaa cagttattaa atattaggag
    13321 tgtaaaaatt tatgggatta aaagaatttg attttattga agagtctatt gatatgttga
    13381 ggacaatgtc accaacatta gaaactatat cagatgaaat agaagagtat tttgaaaata
    13441 tcttggatga aaagaatcaa gaatatataa atgtaacctc tagaataaaa tcagaatcaa
    13501 gtttaagaga aaagataatt agaaatagat atttaaaaaa atatggagaa gcaagtaatc
    13561 ttattcataa tgtttctgac ttaataggcc taagaattga gtgtagattt attgaggatg
    13621 aaaacaaaat atatagactt ttgagaagat attttaataa aacagatgac aaaataaact
    13681 attataataa agagaataag aatattaaat taaaactatc tgagaggcaa cccaataagc
    13741 aaaaaaatgg atttgaaata tataaaatag atggagtttt tgcgtattta gatagagaag
    13801 taaaatttga acttcaaatt aaatctcttg taaatgtgtt ttggagtgaa atagaacata
    13861 aaataattta taaaaataat acatatttac ttgctgataa atttataaag gatatgatgg
    13921 actctataaa gaataatctt actatgatag ataatcagct tttaagtatt tataaaaact
    13981 ttcattcagg gaagtctttt aatatgaaag taagtaaaaa agaaattgaa aaattatttg
    14041 cgaaactagt ctatgacgct ttttctgaaa agatgaataa aagtataggt tttgttgtag
    14101 attttaaaaa accatgtgaa actatattga actattcttt taataaacaa gatataagtg
    14161 atgaagtttt aggtaatttt atgcttgatg agtttgcaag attaaatgaa attgtaaaca
    14221 aggatataga ttttaatgag caaatagaat ttgaaagaga accaacattt gatgataagt
    14281 tttgtaaaga cctaggaaat cattttagaa gtagattaaa cacagaattt ccatggaacc
    14341 tattttttag aatattattt gaaatagagc catataacaa taccaaagac tttgaaaact
    14401 ttgtagagtt tataaaagaa aatgtgctac tagaagaaaa tagggagcaa ttattatgcc
    14461 aatttgaaga agatagcaag cttataattg aagatatata tgattgtata tttaatagta
    14521 taagtgagat tgatagtgtt gaaattttat ataactataa tcttgaaaaa ataaacttta
    14581 catcaagtga aatcataaat tgtatatgta gagaatatga gtgttatggt gaatacttag
    14641 aagaaaaaga aaagcttatg aatacattta aagaaaagat tattcaaatc tttgagtaat
    14701 aggtcaatgc tgaagtggcg ctaatgcgcc atttttcaaa tatatatgaa ttaagtaaaa
    14761 ttattttgga ggaggcgatt tgtatagaga aaacaggtgt aattttggca gggggaaaaa
    14821 attctagaat gggtagagat aaagcctttt tagagttaca tgataagctt tttattgaaa
    14881 ttgcaataga agcatttaaa aactttgatg agttgataat aatatctaat aatgaagaat
    14941 tatattctaa atacgatatt aaagtttata atgatatagt aaaagatgtt ggtccaattg
    15001 gaggaatata tactgctctt gaatatgcaa agtatgatat agttacaatt gcatgtgata
    15061 tgccatatct aaataatcaa atagtcgaaa gaatagctaa taaaatgaat gataaaagtg
    15121 tgatttcagt gacaaatgga aagttacaac cattgtgttc tggatataaa aagagtataa
    15181 taaataaagt atctttatgt ataagggaaa atgatttaaa gctcagaagc tttatagata
    15241 aaatagataa aagttatata tactttgatg aagaagattt atttttaaat gtaaacactg
    15301 ttgatgagta tgaaaaatta actaaagttt aattagctat ggttgtaaaa tagagtagga
    15361 tttatatgga ggagagttta tgaatttact taaatgcgat tcttgggcag taatattaaa
    15421 taatagcgat aaagaaagta aagcatataa aatattagat gaacttaaaa ggaatatgta
    15481 caaagttgtt gctatagatg aagagaaaaa acctattgaa ggaatagatg tgtatgaatg
    15541 tttaaaagat gttccacaca atattgatgt tgtagctatt attgataaac aatctaagat
    15601 ggatgtgatt ttagaagaag tagagctttt ggatatacaa aatatgtggt ttgaaaaagg
    15661 aagctttagt gaaatgatga ttaaaaagac taaagattta aaattaaata tagaatataa
    15721 tttaagttta tatgatgagt tgactagata aaattatatt tcttaataat aaaaatataa
    15781 ttagttaata aaacatatta ttttatagta tagattggtt aataaaagta cagattggtt
    15841 aatgaaacat taaattacaa tatttgttaa aagaattgtg agttacatga tataatgtat
    15901 taaataggat actaaggagt tgatatatta tgttaatagt aactacagaa aaagtagaag
    15961 gtaaaaagat atcaaaggtt ttaggattag tgagaggaag tacaataaga gcaaaacatg
    16021 ttggaaaaga tataggagca agttttaaga atcttgttgg aggagaactt actggatata
    16081 atgaaatgct cactgaagca agacaaattg ccataggtag aatggttgaa gatgcagaag
    16141 ctaaaggtgc aaacgcagta atagcattta gactgtcttc agcttcagtt atgcaagggg
    16201 cagcagaaat gcttgcttat ggaacagcag ttgttttaga agatgataat agtattcttg
    16261 aaaaataaaa tataaatagt taaaaagagg catcttttaa aaattttatg gatgcctttt
    16321 tttatttgaa aaaactaatt tgagaagaaa ttttaactat tatgacaata aaattagggt
    16381 gatattatat aaataagtaa aattatatta tataatgtat ggtatagact ttaaaaattt
    16441 tatttgtaga gaaaaatatg gacaatgtta taataattat agaaattaaa agtacttaca
    16501 ttcattatgt atgtgacatg tataattagt tttatattta atacagtttt gaagtaatat
    16561 cttcattttg agaacgttgg aataataaaa aatgtatgta ttataggtga tataaataaa
    16621 aaataaatat aatatgttat attgctgata gaaaagagaa taaaagaaaa taggttttat
    16681 taaaagagat ttttatttat aggacttaat caatgtatag aggggtgaga acatatgttt
    16741 aatgttgcaa ttataacact tagtgataaa ggatatgaag gaaaaagaga agatataaca
    16801 ggaaaaaagt taactgaatt tgttgaaaat acaggagctt ataaagttac tgagtatgta
    16861 cttataaaag atgataagga aatgctaaag gaaaatatta taagactttg taatagtaat
    16921 aaaatagatt taattcttac aaatggaggt actggttttt caaaaagaga tataacccca
    16981 gaagcaacta aggaagtgtt ggaaaaagaa ataccaggtc tgagcgaata tatgagaatg
    17041 aaatccacag aaattactaa aaaggccata ctaagtaggg gtgtgagtgg aataagaaat
    17101 aattctataa taataaattt acctggaagt cctaaaggtg cagttgaaaa tctaagtttt
    17161 ataattgatg ttttagacca tggcatagaa gttttaagag gagaagctac agaatgtgcc
    17221 actaaaaaaa gtgaatagta tataatctag atagataaaa aatagacaaa gaggtagata
    17281 aagtatgatt gataagtatg gaagaaaaat agactacctt cgtatttcac ttacagataa
    17341 gtgtaattta aggtgtgtct attgtatgcc accagatgta aaatttgata aaaattatat
    17401 aaatgaaaat ctaagctttg aagactacaa gtttattata aaggctatgg ctgaacaagg
    17461 tataacaaaa attagattta ctggaggaga acctctttta tatcctaaat taaatgagct
    17521 tataaaattt acaaaagaag aatgtggaat aaataacata ggaatgacta ctaatgctat
    17581 tgggctttct gatagaattt ttgagcttga aaaatgtggc ttaaataaag ttaatataag
    17641 tttagattct ttgaaggaat ataagtataa atccgtaaca agaggaggaa acttaaaaga
    17701 tgtcttaaaa tcgattgaat cttgtttaaa cttaggtata aaagtaaagt taaattgcgt
    17761 tgctataagt gggtttaatg atgatgagct taaagatttt atgtttttaa cagttaaatc
    17821 accaattgat gtaagattta tagagcttat gccattaggt gaggctaata gagtttataa
    17881 gaagggttat tttaatgtaa aagaaatgat tgaaaaaata tatggtttat ataaagtcaa
    17941 aaatgatgaa aaaagtacag cagaatatta tatgtttaaa ggagctaaag gaagaatagg
    18001 aattataact ccattaagct gttcattttg taacacttgt aatcgtatta gacttacttc
    18061 tagtggtgca ataaaattat gtctacattc aaaagaagaa atagatataa aaccattttt
    18121 acataaacca ttgttattta gagaatctat gaaagatata ataaaacaaa aaccagaaaa
    18181 acatcattta atagaaaata aatgtagtga tacaaatagg cgtatgtatg aaattggagg
    18241 ataagtaatg ttaacacata taaatgaaaa agggtatgcc aagatggtgg acataagtga
    18301 aaaagatgat actaaaagaa cggccgtagc tacagctaca attactctta gtaaggaagc
    18361 attagaaaaa gtaaaaaatg gtcagataaa aaaaggagac gtattgtctg tagctcaagt
    18421 tgcaggtata atgggagcta aaaatacatc ttcaaacata ccaatgtgcc atcagattaa
    18481 attagttgga tgtgatttag aatttgatgt agatgattat atgtctgcta ttaagatata
    18541 cgcaacctgt aaatctcttg ggaaaactgg agtagaaatg gaagctttaa atgcatgttc
    18601 tttagcagca cttacaattt atgatatgtg taaagcagtt gataaaaaaa tggttataag
    18661 taatatccat cttgtggaga agactggtgg aaaatcagga gattttaagt tcgagtaaat
    18721 taataatata ttgggttaaa atttggagga aatatggcga aggtagtgtc aataaatata
    18781 agtgagaaaa agggtgtaat taaagttcct gtaccatgtg cagagctaaa aataaatcat
    18841 gggatagttg gagatgcaca tgcaggaaac tggcatcgtc agataagctt acttggaaaa
    18901 gaaagcatag ataaaatgaa aatgaaaggc tttgaccaat taaaatatgg agattttgct
    18961 gaaaatgtaa ctacagaagg gatagaagtt ttttccctgc ctgttggtac aaagttaaga
    19021 atagggcaat gtgaagttga agtaactcaa atagggaaaa aatgtcacaa tggatgtgaa
    19081 attaagaaat tgacaggtga ctgtgtgatg ccacgagaag gaatttttgt aaaggttcta
    19141 aaagaaggaa ttataaatgt gaatgattca atagatgtaa tctaatgaag tcactattaa
    19201 aatataatta taagtaaata ctattaagat ttaaatatat attttaagag agaattaatt
    19261 taaataaaat attttagtta taaattggaa tattggttat ataaagctat gaatgtaaaa
    19321 tcatagcttt ttgtttttga a
//
LOCUS       DAELSY010000071        19277 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.071, whole genome shotgun sequence.
ACCESSION   DAELSY010000071 DAELSY010000000
VERSION     DAELSY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19277
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.071"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(427..849)
                     /locus_tag="KPG09_002196"
     CDS             complement(427..849)
                     /locus_tag="KPG09_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBG0966805.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            1327..4230
                     /locus_tag="KPG09_002197"
     CDS             1327..4230
                     /locus_tag="KPG09_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0966806.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILVL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     ISIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            4263..4709
                     /locus_tag="KPG09_002198"
     CDS             4263..4709
                     /locus_tag="KPG09_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0966807.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            4832..5134
                     /locus_tag="KPG09_002199"
     CDS             4832..5134
                     /locus_tag="KPG09_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0966808.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            5315..6721
                     /locus_tag="KPG09_002200"
     CDS             5315..6721
                     /locus_tag="KPG09_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG0966809.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFSDPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVISMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            7065..8123
                     /locus_tag="KPG09_002201"
     CDS             7065..8123
                     /locus_tag="KPG09_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG0966810.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYASPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            8198..8884
                     /gene="araD"
                     /locus_tag="KPG09_002202"
     CDS             8198..8884
                     /gene="araD"
                     /locus_tag="KPG09_002202"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBG0966811.1"
                     /translation="MLEILKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNVVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            8902..9549
                     /locus_tag="KPG09_002203"
     CDS             8902..9549
                     /locus_tag="KPG09_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG0966812.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GIGREELNVEHICVLDLNGNVIEVEKGIKPSSEVLMHIEIYKIRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFESWKYPEEIKF"
     gene            complement(9722..11089)
                     /locus_tag="KPG09_002204"
     CDS             complement(9722..11089)
                     /locus_tag="KPG09_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBG0966813.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLRSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(11279..11367)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            11795..13915
                     /locus_tag="KPG09_002205"
     CDS             11795..13915
                     /locus_tag="KPG09_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBG0966814.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESILKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNKLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKEIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            14019..15272
                     /locus_tag="KPG09_002206"
     CDS             14019..15272
                     /locus_tag="KPG09_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBG0966815.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFEDIETYSKDSQKKREKEL
                     DKFINTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEIYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTQV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGFMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(15426..16487)
                     /gene="ytvI"
                     /locus_tag="KPG09_002207"
     CDS             complement(15426..16487)
                     /gene="ytvI"
                     /locus_tag="KPG09_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG0966816.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLSLILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFLELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFIGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            16761..17501
                     /locus_tag="KPG09_002208"
     CDS             16761..17501
                     /locus_tag="KPG09_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBG0966817.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            17513..>19277
                     /locus_tag="KPG09_002209"
     CDS             17513..>19277
                     /locus_tag="KPG09_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0966818.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRILEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFV"
BASE COUNT         7752 a         2071 c         3225 g         6229 t
ORIGIN      
        1 gaactaagta gtatagattt atagataaaa aagatattga gagttttatg gaagagggga
       61 atatattttc tattagagat aagaatatga catatatttt ttattgattt gtacaaaaat
      121 actatatagt gaataaatta atatcttttc taaattagaa gaggataatc ttattagata
      181 aagattatcc tctttattga aagtttaagg gatattagag attattaatt tttctctctt
      241 tttttaacta aaagcaataa tacataaaat tattattgct aatgatactc ttaatgtggt
      301 atatagtcat agtgtcacat gtttatcaat agttttttca ttaattattg taacgaatag
      361 taggactcat aaaatactta gaaatatgtt tttagataga ttttaaatat ataatctaac
      421 ttatatctat aaattaacac tattattctc caattccatt tctagttttt ttataaattt
      481 attaaattct tctggttgcc tttttccaaa caatactctt acaagagctg aattgatttt
      541 tccaaatata ttcctcttgc tctcactttc aataaaaata agttttgtat tttctaagtc
      601 aatatgtact aattcatatc tagtaatagt actttctctt ttattgcttg tttttatctc
      661 atatactcta tttttttcga aattagttat ttcaacctta aatcttttat tatttttttg
      721 ttgtacaaat gttcctactg cttttttctc attaaaatta ggaaaattta gtcttgcaga
      781 ttttgtaaat atattaaata tgtcttttgc agaatatttt aataaaactt cagttttaaa
      841 tgtattcata tcgttttccc ttctgtatat aaatttaata tttataagtt aatttttaag
      901 ttaaaatagt ataatatgcc atattaaatc atgttaaaac tatttatata gctaaaacct
      961 tacctatata agtatgtata ctatcatata aaaataaaat cttaaaatag aaagggttaa
     1021 aaacacagtt tattataatt tttattgttg agtagatacg tactattata tatgattgag
     1081 caagtttttc aatcataaat ttataaagta ttttgcatta aaatttaaac aaagagttaa
     1141 attatttaag attatgaata actaatacat ttagtaaatt actaaatata caaaatgtaa
     1201 atacttatca aaatgtataa atacttatga catctagtta cttacagggt ttaagtagaa
     1261 aaaaatatca aagtaagtat ggcatgatag ttgcttatat atttatcaag aaaggagtta
     1321 ttaagcatgg agttgagaga taaacttgta aacatcataa atacagaaaa taaaaaagat
     1381 ccattaacag atgttcaaat agcaaagttt ttaagtacaa ctagagagaa cataactaat
     1441 ttgagaaaag aacttaatat aagtaattca aggcaaagaa gatatcctta tttaaaatca
     1501 gcaatttcgg caatattaca aaagaataag aatatttcta tatcagaaat tacaagggaa
     1561 ttgatgacag aaggctttaa tatatcgaga agagttgtgg aggaactttt acctaaagaa
     1621 agtttagaaa tggatgtagt agaggaatct gaagaagaat cgaatgaccc atttgaaact
     1681 ctgataggta ataaagggag tcttagaaat gctgtggagc aggctaaatc tgctatattg
     1741 tatccaccaa aaggattacc aacgttgatt attggggaaa gtggagttgg taagagttta
     1801 ttttctagac atatgtatga gtttgcaaag caaaaaaaga tagtaaaaga aagctccaat
     1861 tttgttgtat ttaactgtgc tgattatagt gacaatccac aattattatt atctttgtta
     1921 tttggatata aaaaaggtgc atttacaggt gctgaatatg atacaccagg tcttgtagaa
     1981 gaagctgatg aaggagtttt atttttggat gaaatacata gattgcctcc aaaaggtcaa
     2041 gaaatactat tttctatctt agatagggga aagtttagga gattaggtga aactaatgct
     2101 gaaagaaaag taagtattat gttgattggg gcaactactg aaaatgtaga gactaattta
     2161 ttattaacat tcagaagaag gataccaatg ctaataactt tgccaccatt acatgataga
     2221 ttactaaaag agaaaataga ccttatatac aatatctttc aacaagagtg taatagaata
     2281 aatgcaaaaa tatttgtaga taaaaatgtg attgaaatac ttgtactaaa aaagtttagt
     2341 ggaaatatag gacaattaca aaatatgatt caggttcttt gtgcaagagc ttttatgaag
     2401 tttataaatt ccaaagatga agatagtgaa tcgatagtag tagtagatat aaatgaagtt
     2461 ttaaagctta aagattcttt taaagatgct gcctttcaag agattgaata tacagagata
     2521 agaaaatatt tgaaaaatgc tatatttata ccatttaatt tagaagaatc atcagtaaaa
     2581 ggaagtcttt taagtgatga atatatatta ccagaggaca tatacaaaac aattgaaaag
     2641 aaatattatg acctaaaatc cctagacatt gaagatatag atatagagaa tattttatgg
     2701 acttttattt taaatagatt tgctgactta aagtttaatc tcgatagtaa tgaagaaatt
     2761 ttttcaatga gtgatttaaa tagttttgta agtgatagtt tagttaaact ggtaaaagaa
     2821 cttatgaatg atatagttga aaaaaatatg aatttagaag taaataaaaa tatgttcaaa
     2881 tatctagcaa tacacttaga agaagctata aaaagaatta agctaaatca aaagataata
     2941 aatgtaaatt tagaaaaaat aaagattgac ttttcaaaag aatatgaaat tagtaaaagt
     3001 tttgcaataa aaattgagga atcaattagt attaagattc cagatgatga aattggattt
     3061 ataacactct acataaagtc tgctctaaaa aatgaagtga aaaaagataa agtagggtta
     3121 ataataattt ctcatggacg tatagctaca gagactgtaa atgtagttaa agaactttta
     3181 ggtgtaaagt ttcctgtggc tatagatatg cctttagatg aaaaaccaat aaatatatac
     3241 aataaggcag tagaactgtc aaaaataata gaccaaggga aaggtatatt gtttttggtg
     3301 gatattggtt cacttacaaa cataggtcaa atagtaaata agagaaccaa tataagcact
     3361 aaaactattg atagagtaga cctcctaatg gcactagaag caactagaaa agtttctatg
     3421 ggagaggaag aacttgatga aatatttttt tctttgctaa aagataggat gggatataac
     3481 tatgaattag aaaaacacat agataaatca aatgcaattg taacattatg tcttactgga
     3541 gaaggaactg ctaagtatat aagtaaaacg ttggaagaaa agtatgataa cactaaatgt
     3601 tatcaaatga gtgcattgga tgaaaattta ttttgtaaaa ttgaaaatct aaaagagaca
     3661 aacaatatat tagctattgt tgggacaata aaccctaaga taccaggtat aaattttata
     3721 ccctataata aagaactttt caaaaatttg gatatctatc tatcaaaatc aaaacaacaa
     3781 gatgacagac cagttaaatc gtatgaaaga atgcttgatg aggatttagt gatttttgaa
     3841 ccagatatat attttaaaaa ggatttatta gaatatgtat gttcaattct tataaataaa
     3901 gaatatgtag aaaaagatta cttagattct gttttacata gagaagaaat gcttccaaca
     3961 tattctaaag gagctatagg tgtgcctcat ggagattctt ctactgtaaa tagtacaagg
     4021 tttgtttttg ttaaactaaa gaatcctatt gattggggtg taggtaatgt aaatttcata
     4081 tttatgccag tttttcaggc taatgataaa gagattgtta aaaatgttct tgagatacta
     4141 aaagatgcag aatttatgaa caatgccaat aagtgttttg ataaagatgc ttttagaagg
     4201 attatatttg ataaatttaa acatctgtag ccatagcaaa aaaattgaga ggagttagag
     4261 taatgaatgt agatttaata tcattgggtt tagaatctaa tgattatgaa agtgttattg
     4321 aagaattagg aagtataatg tgtaaaaaag aatatgtaaa agaaacatac ataaatgctg
     4381 tcttggaaag agaaaggaca ttaccaacag gtctagatat tggagaaatg tgtgttgcta
     4441 ttccacatac agattcaaag catgtaaatg agtctaatgt agctgtaggg atattgaaaa
     4501 atcctgtaaa gtttaattca atgatagacc caaaagatag acttgatgta gaacttgtat
     4561 ttttattagc agtaaagaac ccagattcac aagtaaaact gttaaaagat ttaatgtcag
     4621 tttttcaaaa tataaagtta ttaaaaaata taaaaaacgc atcaacaaaa gaagaagtag
     4681 ctaaattatt ggattttata gaaatttaat gtaggagaat tttataaata aaaaattaaa
     4741 atttcaacaa tcaaagtaag tattattttt ttaataggtt taatcttata taaaataaaa
     4801 atttagagtg aaaaattagg aggaaattat tatgaaacaa tttaatgtat tatcagtttg
     4861 tggttcaggg acagttacat catctatggt agcaggtaaa ttaaaagaag tactaggtaa
     4921 taaggggatt tctattacta caacagaagc tagaccaaca gaagcactaa atttagcaca
     4981 atcaggaaga tttgacttta taacatttac gagtccgctt caaacaggcg attatggaat
     5041 accaactata aatgcatttg cttgcctaac tggtataggg gaagaggcat tctttgaaga
     5101 agttttgact gttattgaag gcatagataa gtaagttttg gagacgtgaa tttatagtat
     5161 ttaaatcaat gatttagata ctataataag ctaatattaa ctaaatattt gtggaaaaaa
     5221 cattttatat gttgtcataa attaaaaaat tataaaaata gtcacttaag tattaaatta
     5281 agcttataaa tatttataag taggagggaa aaagatgtta gaatttatga aatctataat
     5341 cgatacattt ggttctgcaa ttatagttcc aattataata ttcataatag caaagatatt
     5401 taaagtaact actaagaaat cgtttctttc tgctgtgtat gcaggagttg ctttacaagg
     5461 ttttacactg attttaaatt catttacacc aataataaca ccagttatca ataggatggt
     5521 tgagagtaca ggagtaaacc taccagtttt tgatgttggt tggcaagcaa catcattggt
     5581 tgcattttca acaagtgcag gtatgatata tttaggtctt ggtatattat tacaaacaat
     5641 tttatttttg ataaaatgga cagacgtgtt tcaaccatca gatttatgga ataactattc
     5701 ttatatggta tggggagcaa tggttatagg agtaacagga aattttcctt taggtatagc
     5761 atgtatggtt ttattaaact tatatagttt attaatatca gagttagttg caaagagatg
     5821 gtctagttac tatagatatc ctaattgtac aataatagct atgcataatg ttgaggcttc
     5881 tgtgtttgca gtattttcag accctatata caataaattg ggattaaata aaataaaatt
     5941 aaatccaaaa gaattagaga agaaattagg gtttttaggt gaaccaatta ctttaggtct
     6001 ttttttaggt atgtttatag gtatattagg taatatgacg agaataaata ctatggaagc
     6061 ttggggagaa ataatgaaag taggtatatc tacaagtgca gtgatggcga ttttccctaa
     6121 agtagcaagt atgtttgctc aagcatttgc acctataaca gaagcagcta gaaagattat
     6181 gcaaaaagct ggaaaccgtg agtggtacat agcagtaaat gatgctgttg gatatggtga
     6241 acctgcaact ttaatatcag gtcttatatt aattccaata atgcttgtaa tatctatggt
     6301 acttcctggt aataaggtat taccagttgt ggatttgttg gcaattccat atatggttca
     6361 aggtttagtt gccatacata atggtaatat accaaaagta ttagtttcag gtataatttg
     6421 gtttggatta ggattatatg tttgtacatc tacagcacca ttatttacag atatggctac
     6481 aaatataggt gtagctatac ctgcaggagc aatgttaata acaagcttta atatactagg
     6541 aaaacctttg atgggattag ttttctttgc atttctaagt gcaaatccaa tttatatagg
     6601 tctttcagtt gtaatttact ttgttctatg ggcattgttt agaaagaaca aaacaagtat
     6661 tcttgattat ttagaaaaac aagcacttaa aaatgtagaa gaagagcctg tagcagttta
     6721 agtttataaa aatgaaataa gaataattaa aggagtttat aattgatttt atataagtat
     6781 cagataatac ctgagaaata tttaaatgaa atatagactt ctttttattt gataatattt
     6841 gtaattataa tgcattgtat ttttatagtt gagttttctg tataacctgc tatttaaaac
     6901 atactctttt atatttatac agtaatgtgg tttaatttta taaaatgtaa tctctactgg
     6961 taaaagactt tgtatataag gcatttgcct gaataaacca tttatctttt gtatggcata
     7021 gatattgcta tatagaagta tatagcatga aaggagatat ttatatggga gataaaatga
     7081 gagcatcagt attgtataat gtaggtgatg tcaggtatga aatggtagat ataccagaaa
     7141 taacagatac gcaagtgctt gtaaatgtaa aatatgttgg gatatgtggt tcagatttgc
     7201 ctaggtctat ggtaagtggt ttgagtggaa atacaaaata tccattgatt ttaggacatg
     7261 aattttctgg tgaagttgtt aaaattggag aaaaagtaaa gcatattaat gtaggagata
     7321 gagtagctgt tgcaccatta gtaccttgtg gaaaatgtga ttattgtaat gaaggtaatt
     7381 ttggcttgtg tgatgattac aatataattg gaacaagagt aaatggagcg tttgcagaat
     7441 atgtaagagt tccagaagaa catattttaa aactaccaga tacattggat tatgaaactg
     7501 ctgccggaat tgaaccagca actatagctt atcatgggat ttcaaagtct aatattagag
     7561 taggagatag tgtagttgtg ttaggatgtg gtccaatagg tcagtttgtt atacaatggg
     7621 caaaggtgtt tggtgcatct aagattatag cagttgatat ttttgatgaa aaattagaac
     7681 tttctaaact tctaggagca aactatatac ttaactcaaa agaagtaaat gtaatcaaag
     7741 aaataaaaaa aataacaaat ggtggtgctg atgttgtaat agagactgct ggtagccgat
     7801 ttacacaaga acaatctcta tttgtagcaa aaaagagagg aaatatagtg tttgtaggta
     7861 tatcacacac agaattacca cttagtgcag acgctactga gtgtatatta cgtggagaac
     7921 ttaccttgaa aggttcttgg aattcatatg caagccctta tcctggaaga gcatggactg
     7981 caacattgga ttttatggga aaaggtgaca ttatttttaa acccatgatt tcacataaaa
     8041 taggtcttaa tgaagtgggt gactttttaa gtaaaatgag caagagagaa attaatttca
     8101 ataaaatcct agtagaaata taaaaattta ataaaaaatt ctaaaaacaa ttaaatttta
     8161 taactatgac attaaataaa atataggagg aagaaaagtg ctagaaattt taaaagaaga
     8221 agtattaaaa gcaaacttag aattacctaa gaaaaagcta attacttata cttggggaaa
     8281 tgtaagtggg atagatagag aaaaaggatt agtggtgata aaaccaagtg gtgtagagta
     8341 tagtaatatg actgtagaag atatggttgt agttgattta gagggaaatg tagttgatgg
     8401 caaactaaga ccctcctcag atactccgac tcatctagtt ttatataaag agtttgaaga
     8461 attgggggga atagtacata cacattcaag ttgggcaaca atttgggctc aaataggaaa
     8521 atctatacca tcttgtggta ctactcatgc agattatttt tatggaagca taccatgtac
     8581 cagaaaaatg actagagaag aaatttgcaa tgaatacgaa aaggaaactg gaaatgtaat
     8641 cattgaagaa tttatgggaa gaaatccaat tcattgtcca ggaataattg taaatgacca
     8701 cggacctttt acatggggaa aagatgcaaa tgaggctgta cacaatgcag tagttttaga
     8761 agaagtcgca aagatggctt attatacaga gttaatgagt cctgataata ttatggataa
     8821 ggttcttatg aataagcatt tttcaagaaa acatggaaaa aatgcatact atggtcaaaa
     8881 atgattatct ggaggtgaat tatgctagac tctttaaaag gtaaagttgt ggaaatagca
     8941 attaaagcag agcattatgg tttatgtaaa gaaaagactg gtaattttag tattagagat
     9001 aaggatactg gatatatact tataacccca tcaggtatag gaagagaaga attaaacgtt
     9061 gaacatattt gtgtattgga tttgaatgga aatgtaatag aagttgagaa aggtattaag
     9121 ccaagtagtg aggtattgat gcatattgaa atatataaaa taagggaaaa tataaatgct
     9181 atatcacata ctcattcttt atatgcaaca gcattttcag tagcaaagaa accaattata
     9241 ccaattgtat atgaatcagt taattatggc ggttatgtag atattgctcc atacgaaaga
     9301 ccaggaactt taaaattagc taagagcgtt tcaaaattac tgatagatac agatgcatgc
     9361 ttattagaaa gacatgggtt gacaactgta ggtaatgatt tagacgaagc attattaaaa
     9421 tcaagatatg ttgaagaggt agcagaaata tactataggt ctttagtatt aaatcaattt
     9481 aaagaacttg aagtagtaga tgttgatgag tttgaatctt ggaaataccc agaagaaata
     9541 aagttttaat ctaaataatt ttcattgaag tggttaatta aaatatatgt attatttata
     9601 tcaattacta tgttttatta tattaatcac cgtattttgt aaatatattt attacatttt
     9661 ttcaaaaaaa tatagaatcc gaaagccata taaaattgac ttgggattct atacttttta
     9721 tttatgcatt tttctttatc tgattttctg gagaatttat atgttcaaaa tattttttag
     9781 tttcagatac tattataggt gataatgcaa gtaaagctat aaggttaggt attgccataa
     9841 gcccatttac aatatcagca attatccaaa caacttctaa ctttaagaaa gcacctaata
     9901 acaccatagc cacaaataca gttctatacc cattcatgcc ttttacacca aacagaaatt
     9961 caaaacatct ttctccataa taactccatc ccaaaattgt agtaaatgca aataatgtta
    10021 aactaactgt aagtaatatt ggtccaaatg taggaattgc tccgttaaaa gctgcttgtg
    10081 tcataacagc tccatttagg tctgaacacc acactcctga aataacaaga gagaatccag
    10141 taagagtaca gattataata gtatctataa aagtacctgt catagatata agaccttgtt
    10201 ccgctggcca ttcagtttta gcagcagctg cagctattgg agcactacct agaccagact
    10261 catttgaaaa tacaccacga gcaataccat ttctaattgc cagtgacata gttgctccta
    10321 aaaaaccacc agtagcagca gtaggtgtaa atgcactttt aattattaac ataaaagttt
    10381 cagggatttt attccaaaaa gatattaaaa tgattccaca gattacaaca tatataactg
    10441 ccataaaagg tactatttta gtagctacat tagatatact tctaagtcct ccaaatacaa
    10501 ccaaagctac aaatactgct aatacaaccc ctgtaattct agggtctatc ccgaaactat
    10561 tatttattgc atctgttatt gaatttactt gagtaaaagt accgattcct aaaagtgcaa
    10621 ctagtatacc acttatagcg aaaaatattg caagaggtct ccatttttct cccataccat
    10681 ttactatgta atacattggc ccaccagaca cttgaccatt tttatcttta gttctgtatt
    10741 ttattgcaag aacaccttct gaatacttag tagccattcc aaagaatgct gctatccaca
    10801 tccaaaaaag tgcaccaggt ccaccagctt tgatagctgt agcaacacca acaatattac
    10861 cagtaccaac tgtagcagct agagcagtac ataaggctgc aaaacttgaa acatcaccac
    10921 tacctttgtt ttcagcagag aatattaact taagagctct tggcagttta gttatttgaa
    10981 caactccaag cttaacagta agtagtatac cagtaccaac taatagtacc agtaggggtg
    11041 gtccccaaat aaaagcatca actttgttta aaatatcaat tagattcata aaaacactcc
    11101 ctcatttttt ttgctttaca taatcaatag aatttttaat gaagtaattg acaggaaata
    11161 agtataattt attaataatt tctagtgtaa taaaaaaagc taaatctaaa aaagattcag
    11221 ctctggatgt acactaatta gcaaccaaaa taaaagtatt agtaaatact gtttttggta
    11281 gataatccct gtccttttgc ctgagagatt gagcataaaa gccttgcccc ttcggcgccc
    11341 ataatgggtc tctccagagt tccgtaaatt ttacagtcct acttttaaaa gtacctgaga
    11401 gtgttactcc ttcggtgtgc tatatagcaa ctcttctata aaatcttaac cggatattga
    11461 tttgtcatag cttattgtat taataatatt aatataagtt tttatgtttt gtcaactaaa
    11521 aaattaaaaa acaaagaaaa aatataaaaa tgtaagccta tttacgaatt ataaacttag
    11581 aaagatgagt aatatacgta aaaatgaatt ttgtaataca catacaaatg aagtgtaaaa
    11641 taagttttaa ataaaaaaat aataaaatct taaataatta attagatatg attaaattat
    11701 ggtaatatta tagttacgat attaagaaaa gttctattta gttagatttt aagcgtaata
    11761 tgagattatg tatagagaaa ggatgtattt ttagatggga aaaatattag tgctagcaga
    11821 aaaaccttct gttggaagag atatagcaaa agttttaggg tcaaaaaatg aaaaaaatgg
    11881 ttatatagag gggccaaagt atgtgataac ttgggcacta ggtcatttgg ttacacttgc
    11941 tgaccccgaa agttatggag aaagatataa atcatggagc ttagaagacc tgccaatact
    12001 tcccaagcac cttaagactg ttgtaataaa aaagagtgga aagcagttta atacagttaa
    12061 atcgcaaatg aatagaaatg atatagatga aatagttata gcaacagatg caggtcgtga
    12121 aggagaactt gtagcaaggt ggataataga aaaatcacag gtaaaaaaac ctataaaacg
    12181 tctttggatt tcttcaagta cagacaaagc aataaaagac ggatttgcaa aattaagaag
    12241 tggaaaagaa tatgaaaatt tgtattactc agcaatagca cgagcagaag ctgattggat
    12301 tattggaata aatgcaacca gagctttgac aacaaagtac aatgcacagc tttcttgtgg
    12361 aagagtacaa actcctactc ttgcaatgtt gcttaaaaga gaagaagaaa taagaaattt
    12421 caaacctaaa gagtattatg gattagaatt aattgctaca aagggaaatt cagatataaa
    12481 atttatttgg aatgataaaa acaataattc ttcaactttt agtaaagaaa agatagaatc
    12541 catattaaaa aaagtaaaag gtgtagacat aaaaatatct gaagtaaaca agtcatataa
    12601 gaaaaaatat tcacctgcac tctatgattt aacagaatta caaagagatg caaataaaat
    12661 gtttggattt tcagcaaaag aaactttatc tataatgcaa aaactgtatg aacatcataa
    12721 agtacttact tatcctagaa ctgattctag atatttaaca tcagatattg ttggaacatt
    12781 aaaagataga ataaaagctg taaatattag tgattattca aagatttgta ataaattatt
    12841 aaaaagcaat attatagcta ataaatcgtt tgttgacaat tcaaaggtaa cagaccacca
    12901 tgcaataata ccaacagaac agaaggtatt catgagtgat tttagtgaca aagagcgaaa
    12961 aatatataat cttgtagtaa aaagattttt agcagtacta tctcctcctt ttgaatatga
    13021 gcagacaact ataagtgcaa ctgtagaagg agaaatattt aaggctaagg gaaacaaagt
    13081 aataaattta ggttggaaag aaagttataa agatattgaa gatgaagatg agtatgaaag
    13141 tataccaaat gttttagaaa aagatgtact taaggtcaag ggattaaaga taacttcagg
    13201 aaaaacaaat ccaccaccat acttaaatga gggaacatta ttgactgcta tggaaaagaa
    13261 tggtctaggg acagttgcta caagagcaga tataatagaa aaattattca attcattttt
    13321 aatggaaaag aaaaataaag agatatatat aacatcaaaa ggaagacaat tattagattt
    13381 agttccagta gacttaaaga caccagaact tacagcttca tgggaaaaga aattattgga
    13441 tatttcaaag ggaaaactta gtaaaaatac attcataaat gacatgaaag attattcaaa
    13501 agatattgta ctagaagtaa aaaatagtga taataaattt aaacatgata acttaactaa
    13561 aaatagatgt ccagaatgtg gtaaatttat gttagaggta aatggtaaaa aaggtaagat
    13621 gttgatttgt gaagatagag agtgtaatac tagaaaaact atatcacaag taaccaattc
    13681 aagatgtcct aactgtcata aaaaattaga acttcgtgga gaaggtgagg ggagaatctt
    13741 cacttgtagt tgcggataca gagaaaagtt gtcttctttc aacaagagaa agcaagaaga
    13801 aaaacagaag gcatcaaaaa aagatgttaa tcaatattta aaaaatcaaa ataaaaatca
    13861 agagacattt aataatccat ttgcagaagc attagctaag ctaaaaaaca aataatttta
    13921 aagttattat tatacacaat tataacttta actgtatatt agttatatgt actgatataa
    13981 tatagtgtat agtttaggca tgaaagtagg gctgttaaat ggatttaagt ggcatattta
    14041 aatactattg taaagagtgt gaaaatacat ggaataattc gagtgttgaa ttatttgagg
    14101 atatagaaac gtatagtaaa gattcacaaa aaaagaggga aaaagaatta gataaattta
    14161 taaatacaat atcagttcat ttagagaggt atccaagtga tgctgtattg agaaaaatgt
    14221 gggtaaaaaa gggcgaggtt ttcttacaaa agacattgga aaaagaaaat atttttaagt
    14281 tagaaaaaat ggatgtagag gatagaaaaa aatttttaga tataacaaaa cagtttatta
    14341 gagatgctag aaaatttgat gatgatttac ctataggtga tattatgcaa gctatgagga
    14401 atgtatggat ttcaaatgca ttacaattat tatttggtaa agaaatatat tattcaaaag
    14461 ctaactttgc atatagtatg ttatatccat atactgataa ttatttagac aatacaaata
    14521 tagataaaaa tgataagatt ttatttaata actggttaga aaaaaggctc ctgggagaac
    14581 atattaaatc taaggattat catgaaagta aagtatctca aatgatagat tatattgaaa
    14641 gtgtataccc tagagaaaag tttacacaag tttatgaatc gttattatta atatttaaaa
    14701 gtcaagtaaa tagtttaaaa caacatggta aggaaaatca tttgtgtaaa gaagatttat
    14761 tatccatttc tattgaaaaa ggaggttcat ccgttttagt agatggatat ttaataagtg
    14821 gatttatgac aaaggaagaa atagagtttt gtataggata tggattttta ttacaaatat
    14881 ctgatgattt acaagatata aaagaagatt taaaatacaa ccataaaact attattacag
    14941 agatgtcaaa agagggtact ttagataaag ttgtaaataa actaataaat tttactattg
    15001 agttaataga tagttttaaa attaataata aaaataaatc tgtaataact atgataaaga
    15061 atgattgctt aatgttaatt ttattttctg tagtttataa tgctgaattt ttttctgtag
    15121 gatatataaa agaagtagag aaatttattc catatacaat agattattca ttagagattg
    15181 aagaaaaaat aaaagaaaaa tttaaaaata tagatgtttt aaataatgaa aatgaatata
    15241 aagaaatgat tgatattatt tgtgcagagt agaaaaaaag ggagtgtctt agaatagact
    15301 ttttagttta tgaggcactc tttttgtata aaatcaaatg tattttcatc attttaacaa
    15361 ttaaaaagag cttatccctg tttgagaaag ccctttttaa tttaaataaa aatgtcaagg
    15421 ttaaactatt taaatgcagt aataatccca ttatcatgta agttttttat gattgtagct
    15481 gcgataggga atccaaataa tccagctatt ccaaatagtt gagctccaat aaacatacac
    15541 attaatgtta ttaaaggata taggccaatt tggtgtccta ctacctttgg ttctaatatt
    15601 tgtctaacaa cggttataat tatgtatagt attaaaagtc ctattgctaa attaatattt
    15661 ccattaatga gtgagataat catccaaggt gtcaaaatac ttcctgtacc caagactggc
    15721 aaaatatcaa caagtgctat gatgacagat atagtaaatg cattttcaac ctttaggata
    15781 gtaagtccaa taagtagttc taagaatgtc actgatagta gtattgcata ggcttttagt
    15841 agttttaaaa gagcattaat actgctattt tttatattta ttatcataaa ttgacttttt
    15901 tctggaaatt gttttaagat aaagtttact attttttgat agtcaaaagt aaagaaaaaa
    15961 gaagcaatta aagtaataat aagttttaac ataaatgcag gtgttttact tgcaatacta
    16021 gcaatagcat tgagtgcatt tgttgaaata gaagctacta aagacatcat agattgactt
    16081 atattatccc aaccaagtat taatgatagg ttaacattag gtgtactagc atctatcttt
    16141 gaaaataaag tatttagcgt tggttgtata cttgatttat atatttcagg aagtctaaaa
    16201 aatacatcac ttacacttgc aaatatcttc acaccaaagc taactaataa aaatatacac
    16261 aacccataaa aaattaaaag tattactata gaaataagtg tacgatttaa atttgtatta
    16321 tttgtaataa gtcttataat aggtcttaag atggatgcaa tgataaaact gattacaaaa
    16381 ggcattaata atggcaataa gaattttaat acaatatata caatacttgt tattaaaact
    16441 acatatgtaa atttaattat aaaattcttt tgcttttgaa tatccataat ataaaacctc
    16501 caatgtattt gatattattc taaatatggt cttttttaca gaaaatatac tactgtatat
    16561 tgattataaa ctataaagct tatgtaaagt aaataaatta tgtaaaattt aggttaattt
    16621 tgtataaaga gatactttta tattttacaa ttaataacca aatactaaaa aatattttaa
    16681 ataaagaact taaaattgtg ataaaattaa tttatagata ttaaaaaaag tataatatat
    16741 ggtttttaag aggtgctaag atgaaaaatt ttagagattt aggtgggaac aaaactgagg
    16801 atggcagaac tgtaaagaag ggattatttt ataggtctgc aaaattatca aatctaagtg
    16861 aaaatgatat aaagatttta aaagagttaa atattaaata tatatttgat tatagaagtg
    16921 atgaagaagc tcgtaaacat ccatcaacaa taatatcaaa tataaaaaat ataagaatac
    16981 cagcaatgag agagctagaa gaatcaggag gaagttttgg ttctattgaa gatatgattg
    17041 atggattgtt tgaaaaagat ggtgcattta atatgttaaa taatagttac tataatttgc
    17101 ctataaataa tccgtcatat aaaaaattag tagaacttat aagagactat tctaatttac
    17161 caatattaaa tcattgtaca gctggtaagg atagaactgg tgtggggagt gctataattc
    17221 ttatgatttt aggggtatcg agagagaata taatgaaaga ttatctaaaa agtaatgatt
    17281 ttgcagataa agagatagaa agatttatag aatataagcc taaatttaag gacattccaa
    17341 aagaaaattt aaaatatata tttggtgtaa atgaagagta tatgaaaact gcatttagaa
    17401 gaattgatga agaatacatt agtgtagaag catatttata tggagaattt aatttaaata
    17461 aagaagaaat aagaaaatta agaaatcaat atctagagta aaaggtgagt ttatgagttt
    17521 aaatataaca gcaagacaga taaatataat aaaaatattg ttaaattcaa aagatacaat
    17581 tagtggtatt gccttaagtc aagagatagg atgttcttca aaaactatac aaaatgaaat
    17641 aaaggatatt aataaacaac ttagaaatgg taaaattttg tcaataagag gagttggtta
    17701 taaactagaa ggaagttttg atgaggtaaa tctaaatagc aatctctatg atgatgtaga
    17761 tagagtagaa tatataatca gaaaaatatt aaccttaagt aatgaagaaa aaaatactat
    17821 aaaacttgaa gatttagccg attctatgta tgtaagtgtt tcaacagtaa aaaatgattt
    17881 aaaagaagta aaaagaatat tggaaaagta ttcaatatca gttaaatcta agcataaaca
    17941 aggtatatgt gttttggaaa gtgaagataa gatattaaat tgtatagttg acttaagcaa
    18001 taagagagat aatcaattga gtttaaatga ttttttaaat gacgatataa aggaaagaaa
    18061 actattaata aaaaaattat tattagacat attaaatgaa aatggattag tattaactga
    18121 tatagaattt aagagtgtgt taaataatat attagttatg ttaagcagac ataagtataa
    18181 tgaaaaagaa tttatagaga attatataga aaattatagg agcaaaagaa gctcaatttt
    18241 agaaaatgaa caaaacaaag aaattataat aaattcaata aaaacatttt gtaaaaattt
    18301 aaaattagct acatcaatag atataagtaa tgataaggta tttgaagagt ttttatataa
    18361 gcatattagt agtttatgta aaaaaataaa gttaggtata aatcaatcac ctataatggc
    18421 tcaagatata aaaataaaat atccatttgc ttttgaacta gctaatatag ctaaaaagac
    18481 aatagaaaaa gatttgaaga tagaaattaa tgaagatgaa gttgcaaaca tagctctaca
    18541 tataggagga gcagttgaaa gagcatctta taataataaa gataaggttt tcaaaactat
    18601 aatagtttgt acttcaggca taggcacatc aatgttaata aaagctaaat tagagaatat
    18661 atttaaggaa aaattagaaa tactaaagat tattccttca tacttagttg aatatataaa
    18721 agttatagat gttgattttg taatatctac tgttcctatt gaagtaggag atgtgccagt
    18781 aataaatata tctccaattt taagtgagaa agaaattaga ttaattgaaa aatatataga
    18841 aacaggaaat atatacctag atttagatat aaaaagcctg tttgatagtg aattattttt
    18901 tactgatttg gtgtttaaaa ggaaagaaga agttatagat tttatggcaa gtaaacttgt
    18961 acaaaagggc tatatagatg aggatatgaa aaaatcttac tttgagagag aaaaaatagc
    19021 aacaacagaa atagggaata tggtaactat accacatgga gctattggaa aaatttttga
    19081 taataaaata gcaattggta tattaaagaa gcctatttct tgggaaatat cagatgttcg
    19141 tttaataata atgttagccc ttgataaaga taaaatatta gattatgaag taatattttc
    19201 aaaaatatat aaaagagtag attctatagc aaaagttata agtatatgtg aaaatagtag
    19261 ttttgaaaaa tttgtaa
//
LOCUS       DAELSY010000072        19143 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.072, whole genome shotgun sequence.
ACCESSION   DAELSY010000072 DAELSY010000000
VERSION     DAELSY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19143
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.072"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            161..739
                     /locus_tag="KPG09_002210"
     CDS             161..739
                     /locus_tag="KPG09_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0966819.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            778..1356
                     /locus_tag="KPG09_002211"
     CDS             778..1356
                     /locus_tag="KPG09_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0966820.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLRHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1442..2071
                     /locus_tag="KPG09_002212"
     CDS             1442..2071
                     /locus_tag="KPG09_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0966821.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2061..4820
                     /locus_tag="KPG09_002213"
     CDS             2061..4820
                     /locus_tag="KPG09_002213"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG0966822.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            4907..6469
                     /locus_tag="KPG09_002214"
     CDS             4907..6469
                     /locus_tag="KPG09_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBG0966823.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGVIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDIHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6470..7606
                     /locus_tag="KPG09_002215"
     CDS             6470..7606
                     /locus_tag="KPG09_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG0966824.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7670..8746
                     /locus_tag="KPG09_002216"
     CDS             7670..8746
                     /locus_tag="KPG09_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBG0966825.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHSITGILIPKANSELIENCIEALYSMNLQDMYVI
                     PIIETREFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8724..9962
                     /locus_tag="KPG09_002217"
     CDS             8724..9962
                     /locus_tag="KPG09_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HBG0966826.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGVRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELNCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKKHYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10016..11104
                     /locus_tag="KPG09_002218"
     CDS             10016..11104
                     /locus_tag="KPG09_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBG0966827.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKHFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            11132..11923
                     /locus_tag="KPG09_002219"
     CDS             11132..11923
                     /locus_tag="KPG09_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBG0966828.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKPDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDFSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFKGMSGTEEILSNILDENCLLNI"
     gene            complement(12038..12592)
                     /locus_tag="KPG09_002220"
     CDS             complement(12038..12592)
                     /locus_tag="KPG09_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG0966829.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            12787..13989
                     /locus_tag="KPG09_002221"
     CDS             12787..13989
                     /locus_tag="KPG09_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBG0966830.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            14046..15026
                     /locus_tag="KPG09_002222"
     CDS             14046..15026
                     /locus_tag="KPG09_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG0966831.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            15273..16226
                     /locus_tag="KPG09_002223"
     CDS             15273..16226
                     /locus_tag="KPG09_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG0966832.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RVPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAVFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            16216..17172
                     /locus_tag="KPG09_002224"
     CDS             16216..17172
                     /locus_tag="KPG09_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG0966833.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKMLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            17169..17924
                     /locus_tag="KPG09_002225"
     CDS             17169..17924
                     /locus_tag="KPG09_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0966834.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            18016..18981
                     /locus_tag="KPG09_002226"
     CDS             18016..18981
                     /locus_tag="KPG09_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0966835.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTIKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKTSLPASLEKYKDKKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         7574 a         1938 c         3421 g         6210 t
ORIGIN      
        1 tgtaaaaagt aagaaatata aacatttttt taattatatt ttaaaaatat caagcaagtt
       61 ttcttcacaa atgaataaaa aatgttataa tatgattaaa gaaaaagata aataattagc
      121 aagatttgtt tagctaaaat taacaaggga gcgataaatt atgggtataa cattatcaaa
      181 aggccaaaag gttgatttaa ctaaaggaaa tccaggtctt aaaaatattt tagtaggttt
      241 aggatgggat actaacaagt atgatggtgg atttgacttt gatttagata cagcagcatt
      301 tttaacagga gcaagtggaa gtgttactaa tgatggagat tttgtatttt acaacaattt
      361 aaaacataca tctggagcag ttgagcatct aggagataac agaactggcg aaggtgatgg
      421 agatgatgaa caaatagttg ttgatttatc taagattcct ggagaaataa gcaagatttc
      481 ttttactgtt acaattcatg atgcgacaga aagaagacaa aattttggac aagtaagtaa
      541 ttcttatata agaatagtag ataaagacac aaatgaagag ttaataaagt atgaattagg
      601 agaagatttt agtatagaaa cagctatagt agtagctgaa atatataagc acaatggaga
      661 atggaagttt aatgcactag gttctggttt tgaaggtggt cttgctgcat tatgtggaaa
      721 ctttggaata aacttataat ttcaactata acaataaact aaagggggat attttccatg
      781 gctatagtat taaaaaaagg acaaaaaatc gatttaacta agggaaatcc aggtcttaaa
      841 aatataaaac ttggactagg atgggataca aattcatttg atagtggcta tgattatgat
      901 ttagatgtaa gtgttttcat ggttggagaa tctcaaagag ttgaaaaaga tgaagatttt
      961 atattctaca ataacttaag acatccatct ggagcagttg aacacttagg tgataataga
     1021 actggtgaag gtgatggaga tgatgaggaa atattagtga atttagagac aatgccaaag
     1081 catatacaaa agatagctgt aacagttaca atatatgagg ctgctgaaag aagacaaaat
     1141 tttggacaag taagcaactc atatataaga gtattaaatt ctgaaaatga tgaagaaata
     1201 ttaagatatg acttaggtga agaatttagt atagaaactg caattacagt atgtgagata
     1261 tataagtata atggagaatg gaagtttagt gcagttggag caggatttga aggtggctta
     1321 gaagcacttt gtagaaacta tggcttaaat gtttaattta agcaatataa tatattaaat
     1381 ataaataacc tatacaaata atgtataggt tatattatat aaaattttgt ggaggtttag
     1441 tatgtcgatt aatttatcaa aaggtgataa aatagattta aaaaaatcta acccaggact
     1501 gagtaacata cttgtaggtc taggatggga ccctgtgcaa caatctggag gaggtttttt
     1561 taaatcgtta tttggtggag gacaagctga tatagattgt gatgcatctg tatttatgct
     1621 taatcaagaa ggtaaattaa gtggaataaa agatttaatt tattttggaa atctaaaaag
     1681 tgcctgtaaa tcagttttgc atacaggaga caatctaact ggtgagggta caggtgatga
     1741 tgaacaaatc ctagttaatt tggataaagt tccatcaaat attcataaat tattgtttgt
     1801 agtaaatata tacaattgtg tagatagaaa acaacacttt ggaatgatag aaaatgccta
     1861 tataagagtt gaagaccaag gtaataagaa agagatagca aaatacaatc tatctgataa
     1921 ttattctgaa aaaactactc ttatagtagg agctatatat agaaaagatg gaagttggca
     1981 atttaaagca attggtgaag gaacaaaaga tgcgggtctt aaagaagtaa tgcaaaattt
     2041 agacaggatt gagtgtgcat atggaattta ataatgatat caaatattat aatgtagata
     2101 aagaagaagt tacaaaagac ttatctgtta atcctgaaaa aggtttatca gaatcagaag
     2161 tcaagactag aagagaaaaa tatggtttga atgaatttac tccaaaagaa gagggtagtt
     2221 tttgggatga tttaaaagaa agtttaagtg aaccaatgat agttatcttg attattgcag
     2281 cagtagtaag tgcagtaatt ggggaaactc atgatgcaat agggattgta ggtgctatag
     2341 caataggtat agcaataggt atgattacag aaggtcgttc taagaaagca gcagaggctc
     2401 tttctaagtt gactgaaaac atagaagtaa aagtattgag agatggggaa gtacatcaaa
     2461 tttctaagag tgaattggta ccaggtgata tagtatatat tgaaactggt gacatggtac
     2521 ctgctgatgg tagacttata gaaagtataa accttaaaat tagagaagat atgttaacag
     2581 gagaatctga tgacgtatct aaaaagtgtg atgtagtagt ttctatggaa gagatagaat
     2641 ctaaaggagc agtagtagta caggagccaa tacctgcaaa acagataaat atggtgtttg
     2701 gtggtacact tgtagcttat ggtagaggag ctctagttgt aacttcaatt ggagataata
     2761 gtgaaatggg taaaattgct caaaacttat cagaaacaga tgagcaaact ccacttcaga
     2821 taaaacttgg aaatttaggt ggtttgatag ccaaagtttc tagtgcagta gcaggattac
     2881 tatttatatt tatggttttc caaatgataa gtaagaatgt attaaatgta gatatgtctg
     2941 gaatattacc gtttttagaa tcaatagacc cagttaaaac agcatttaca gtatgtgttg
     3001 cgttgatagt tgcagcagtg ccagaaggtc ttccaactat gataaatatg acacttgcaa
     3061 taacaatgca aaaaatggct aaaataaatg cattggttac caaaaaagaa gcttgtgaaa
     3121 cgataggttc tgtttctgta atatgttcag ataaaactgg tacattaact gaaaatagaa
     3181 tgactgtaga agttgcatat gttgatggca gatatattga aagttcagaa gaggaaataa
     3241 atagttattt tgaagaaaac tgtatgataa actctacagc tgatgtagag cacaatgatg
     3301 gtgatattaa gtatttagga agtgctactg aatgtgcatt gcttttatat tacaaaaacg
     3361 tagattatag acaagttaga aaaaattcta atatagtagc tcaaaatcct tttacttcag
     3421 attcaaagag aatgacttct gttataggtc aagataatca tcatgtacta ttatcaaaag
     3481 gagcaccaga agtcttactt gaattatgtt cacatattca aagaggaaaa gacatagttc
     3541 cattaactga aaatataaaa catgagattt tagaagaaat aaagaaattg caaataaaat
     3601 caatgagaac tttagggttt gcatataaag aaatctcaca agctgaagaa gaagcagcag
     3661 taactgctga aagtgatgct atgaatgtaa gtgcaatgga aaataattta gtatttagtg
     3721 gttttgttgg tataagagac ccacttagaa aagatgttat agagtctgta aatacagcaa
     3781 ataaagctgg tgtttctgta aaaatgctta caggagataa tataaatacg gcaagagcta
     3841 taggtgaaga gttaggccta ttaaaaaata atatgagagt ggttgaagct tcatatatag
     3901 atactttaga tgatgaagaa ttaaaacaag agatacaaag tatttcaatc gtggcaagaa
     3961 gtaaaccaga tagtaaaatg agaatagttt cagctctgca aaagagcggg gatgttgttg
     4021 cagtaacagg tgatggtata aatgatgctc cagcactttc aaaagcagat gttggtatag
     4081 caatgggtat atctggtaca gaggtatcta aaaacgcagc agacataatt ttaactgatg
     4141 atagttttag taccattgta aaaggtatta aatggggtag aggaatttat gataacttcc
     4201 aaagattcgt tcaattccaa ttaactgtaa atgtaattgc atttttagtg gctataatat
     4261 ctcaagttat gggacaagag atgccattta ctacaataca attattatgg gtaaacataa
     4321 ttatggatgg accaccagca ctagcattag gtttagagcc agttagagac catgtgttaa
     4381 atagaaaacc tgttgataga aaagccaata taatttctaa gtcaatggtg tatacaatag
     4441 tattaaatgc attttatata acagcaatac taatgttaca atctacattt aatttcttag
     4501 gagttgatga agctcataag ggaacagtaa tgtttgcgtt atttgctttt agcgcactgt
     4561 ttaatgcatt taactgtaga gagtttaatg cagatagtat cttccctaat tttacacata
     4621 acaaattagc actacaaata atagtgttga caggaattgc acaagtaata tttactcagc
     4681 tattccaaga tttctttaac tctgtagcaa tggattttat gacatgggta aaagttttat
     4741 gtgtagcagc aagtgtaatt gtggttaatg aagttgtaaa atgtataata agactggtaa
     4801 aaccaaaaaa gactgcttaa aacaaatttt ttagaagaaa acagactgac tcaaaatatg
     4861 aattttgggt cagttctgaa taaacattta aattgggagg atagttatgg gtgactcaaa
     4921 ggtatttgaa aaaatattct ccagccaatc agatagagga aattacactc cttcaaaggg
     4981 atatctaagt tattttattt cgtatattgg tttagaagat gaagttttgt acaatctcga
     5041 gatatttaag acaaaacaaa atattgatag caaaaaagat attgctttat ttactgatgt
     5101 tatagcaaat cctagtgatt ttgatattat aaattatttt aaaagtggac ttcaaaaata
     5161 tagaactagc atggaagatg tagatataaa tattttggga tttgaagaga ttgattataa
     5221 aataaagcaa gcaatggata gagtgttaaa agaagaggaa aaagaattta ctaatgatag
     5281 agttaaacaa aattttatag tgaaaataat ggcatggata aaaatttata tcggtgcttt
     5341 agatataaat aaaaatgaag ctccaaaagt aatattttat ggagatataa aaaaacatga
     5401 agtttattta ctcttaatat tatacttggc aggttttgat gttttatatc ttaatccaaa
     5461 ttcaaagagt aatatagata ttttaaaatc agaaagatac aatatagagt ttgaagaagc
     5521 caacataata gaagaaaaaa tttcttttga agaaagggtt attttagggg aaaaaataga
     5581 taaaagttct gtaaaaaaag cttttactgt tggggcagaa gcatcaaagc gtataagtga
     5641 ggaattgtta aatgatgcag gatttataaa accttggcaa ctccaagata gaaaaataaa
     5701 aaatctactt ctaagttcaa ctgttgatga aatatcaata tactggaatc aaccacttaa
     5761 attaagacca ggatttaagt ttaatgatgc tattgtagaa gctccaaatt ttctttctaa
     5821 gattaatgga atatataatg ataagaatga atatattaag tttttagatt tattaagaga
     5881 ttcggaaagt tcaacattta ttgaatttaa tggagatgta gatagatttt caaaagcttt
     5941 tacgagagaa gcttttagtt tatccttttt attggattca aaaggtgtaa tagataaaaa
     6001 ctctgtttta aataataaag attattccat atcaacacta gcacttaatc aacaaattat
     6061 gattttagag aaagtggaag agttgttaga aggaagtatg tttttgaatg gtttaagtgg
     6121 agaagacaag ataaaaggtc tatttacagt acttcacatg gataagaaat ttgttcatat
     6181 gatgaacaat ttcgattact cacttataaa cccaaaattg attatataca tgtacaagag
     6241 tattgtattt gataaagaaa tagtttttct tatgctttta cttagtaaga ttggatttga
     6301 tattatcata ttatgtccag gaggagaaaa caatatagag aatgttataa ataaccaatt
     6361 gattgatata cacagattgg ataaaatggt atatgacctt aaattaaatt cgcttgaaaa
     6421 tgatatacct ttacttaaaa aaatttttgg aaagaggaga cgattttaga tggggataaa
     6481 tataaatagt ttttcaaaag ataagaactc tgtagaggtt actgagaatt tgcctacagc
     6541 agttcaagaa gaaaacttcg atataatgga atatacaaat aataaaaaaa atgaactgag
     6601 aaagtctaag gaagttgagg cattgacatc ccttatagaa gtagaaaatc cagatacgat
     6661 tttacagttt ggtaggaaag catctgaagg agtagcaaga gtttctgata gccttttaaa
     6721 tacaataaaa ttaaatagaa atgaagaaaa ttctaaaatg ctggttcatt taactaaaat
     6781 aatggataag tttgatttag acgattttca agaaactaag gaaccaaatt tcgttcaaaa
     6841 acttttcaaa aaagctaata atgccattga aatgatgttt caaaagtatg aaacattggg
     6901 aggagaagtt gagaaaattc aaattgagct tgagcaatac gaaagagata tagcactttc
     6961 taataagcaa ataggtgcta tgttaaatga aaactttgag ttttataatg aacttcaaaa
     7021 atatattgtt gctggagaaa tggcaataga agaaatggat aatgaaatac tacctttctt
     7081 taaacaaaaa tcagagacta gtggagacca aatggatgtt gtcaattatc aagaactatt
     7141 aaaagtttat gatatgttaa atcaaagagt ctatgattta agaatagcag aaaatatagc
     7201 tatccaaaca atacctatgc ttagaggtat gcaacataat aattatggtt taattagaaa
     7261 aataaatagt gcatttgtcg taactttacc agtatttaaa caatgtttat ctcaagctat
     7321 attacttaaa aagcaagaat tacaagcaaa atctctaaaa gcacttgatg acaaaactaa
     7381 tgaattattg cttagaaatg cacaaaatgt aagtactcaa agtgcacaaa tagctagaat
     7441 ggctggaaca agttctgttc aaatagaaac attggaaaaa atgtacaata ctatcaagtc
     7501 tggtatagat gaaactatga gaatagaaga aaacaataga gctttaatta aggataatac
     7561 aaagagattg gaagaattaa atacgactat aatttataat aagtagtatt atattaaggg
     7621 ctaatttgta taaagttggc cctttctatg tatatgagga ggctagacat tgaaatattt
     7681 cgattatatc tgcaaagatg atttggagag aatttttttg aaggagccag aagactttag
     7741 tgctaagact gaaaaagatg tgttgaaata tgcactgggc gcttttttat acgttccagc
     7801 tactcaatat aatatgattt ataagagtat tataggggat gttaagggag ttagaccatt
     7861 agctatttgt ttagaagatg ctgttggagt aaatggagag ttagaggcta tagaaaatct
     7921 tagattaatt ttaaaaaata ttagcaatga aagtataact aataaagatg gaataccact
     7981 aatttttgta agaataaaag atgtggagca actgttgaga attaaagaga ttataattaa
     8041 aaatagtcat tctataacag gaatattaat tcctaaagca aattcggagt tgattgaaaa
     8101 ttgtatagaa gctttatatt ctatgaatct tcaagatatg tatgttattc caataataga
     8161 gacaagagaa tttatatata atgaaaaaaa agaacttagt tttactaatt tgtacaatgc
     8221 tattttaaga cataagtcaa gaatattaag cattagagta ggcataactg atatattagg
     8281 gatgtatggt ataaggagag ataaaaactt ctctatatat aataatttga tttgttcttc
     8341 atttatatta gatataatca catatcttca aagaccagag ttagatatac ctataagtgg
     8401 tggagtatca gaattttttg atatgacaaa taaggatata agaaataagt atatagaaga
     8461 aatattattg gataaatatc atggattaat tggaaagacc gtaattcatc caatgcaaat
     8521 tcaaattgta caagcgctat ctacagtatc atatgaagat tttactgatg ctttggatat
     8581 actggatagt acagacagta agtatggtgt gagcaagggt gtattaggag agagaatgaa
     8641 tgaaacaaat ccacattttt tatgggctaa aaaaacattg attctatcta aaatatatgg
     8701 tgtattaaat aaaggagtag actatgaaga attgcttaaa ttttaattgt gagattgaca
     8761 ttattgataa tccattgaat ttagaactaa atgatttttt agacgtagga gtaagaaata
     8821 atagcaaaag acgttttttg tttatctcaa agaaactcgg taaacattta gcatgtaagc
     8881 caagtgagat ggataatttg ggaaaattaa tggctacaat ttataatgaa aaaaataagg
     8941 aagaatcaaa tggtactgtt atagcttttg ctgaaactgg tacagctgtt gggcatagtt
     9001 tttttgatta cttatgtgga gattatgaat ttattcatac tactagagag cagtttgacg
     9061 agttagaaaa tctggaattt ttagaggaac attcccatgc aacagaccag aatttatact
     9121 ttggtatgct taacaacttt aaatttggag atgagataat attggttgat gatgagatta
     9181 ccacagccaa tacatgcatg aatataatta ggaaaattca aagcatttat cctaaaaaga
     9241 aatacactat atgctcaata ctcaattggg taagtgatga aaatttaagt aaagttactc
     9301 gtttagaaag agagctaaat tgtaagatag attttgtata cttatttaaa ggaaacttta
     9361 aattttatat agatgaagat attatttcaa attacaacga taattatagt attactaaat
     9421 tagagactat ttcaagtgat aaagtaaatt tagtagtaaa tcatatttat gttgatttag
     9481 aagattatat tggaaataag aaatatgtaa aatatacagg aagatttgga attaatagaa
     9541 gagaacaatt tagattaaaa gaaatcgtta agagagaaag taaaaaaata actatggcca
     9601 gtaaagaatc tttaaaaaat gaaaaagaaa agattctatt tttaggtact gaagaattta
     9661 tgtatatacc tatgctgttt gcaaaagaaa aagaaaatca atatgatata tactatcatt
     9721 caacaacaag aagtccaatt attaatattg ataagaagca ttatcctatt aaaagtaaat
     9781 ttatgctaaa gagtttttat aatatagata ttcaaaatta tatttacaat atagataaac
     9841 ataatttttc taagtgttat ttatttgtag agctgaataa aagtgaaaat tcttatctag
     9901 aatttattga tataattaaa aaaacaagta taaaagaatt aactatagta tgttgtagct
     9961 aaagtgtttt aacaggataa aatatatcac gattttaaga taggagttga gtcatatgag
    10021 agattttagc acgtattcta aagatgatgt tattttttta ttaaaagata tatcaaattt
    10081 aattgaagaa gaaggaaata aagaaagaga attaaaaata caaaatggta gacattattc
    10141 agaaatgata ccaatagagt atgaagttag tgaagattat ctgaatttat atcatgagaa
    10201 actaagagaa aataaagata agcttgcatt ttctatagga gttatgtgtg aaaaaataat
    10261 taaaaaaaat ggaaatgatg tagtactagt ttcacttgct agagcaggaa cacctattgg
    10321 tattttggca aaaagatata taagaagcaa gtataatgta agtttacctc actatacaat
    10381 ctctataata agagataagg gaatagatat gaatgcaatt gaatatatag taaagaatca
    10441 tccgagctca aaaattcaat ttttagatgg atggactggt aagggaacta tatcaaaaga
    10501 acttgaaaaa gcatgtgatg aacttaatgt taaatttaaa aaacattttg actcaacact
    10561 tgctgtatta gctgaccctg ctggatattc aggactttat ggaattagag aagatttctt
    10621 aataccaagt gcatgtttaa attcaaccgt ttctggttta gtaagtagaa ctgtactaag
    10681 agaggattta attggtaaaa atgactttca tggagctaag ttttatagac atttaaaaga
    10741 taaagatgaa tctatgaact atatcgaaac tatagaagaa tgttttaaaa atcaatttaa
    10801 gaatatatct gatgaagttg aaaactggga aagtgacatt ataacaagag atggatactt
    10861 tgatgtttta aatataaaag aaaagtataa cataacagat attaacttta tcaaaccagg
    10921 tgtaggggaa acgacaaggg ttttacttag aagagttccg tataagattc ttgttaaaga
    10981 cttaaatgat aaatctttag accatatatt catattagct aaggagaaaa atgttgaagt
    11041 tgaacaaatg gatttaaaag cctataaatg ttgtggaata ataaaaaata tgaaagatat
    11101 ttaggaaata tattagacag gagaatattg aatgattgtt ttttcagatt tagacaggag
    11161 tatcatttac tccaataaat ttttaaatgc tgatagtaaa tatgctaata tagaaattta
    11221 tagggaaaaa gaaatatctt atatatcttt agatactata aatttaataa agcaaataca
    11281 atattatggt atgtttatac ctacaactac tagaacagtg gagcaattta agcgtataga
    11341 atttaataaa tatgggattt attttccttg gagtataact tcaaatggag gagttatact
    11401 taaagataat gaaatattga aatcatggag tgaaaaaata gataaactaa aacctgatta
    11461 tgagcccatt gaaagtatga ttcataaatt taaagactat ttgaatgtag atggaataac
    11521 taattttaaa gttgcagaag atacattttt ttatatagtt gttgatttct caagatttaa
    11581 tttggattca atcaaagaat atacaaatat attggaatca aaaaattgga agttttatgt
    11641 gagtggcaga aaaatatatt ttataccaaa agaaatttca aaagaaaatg ctataaaata
    11701 cttgactaaa gaattaggaa tagaatattt ttatgcagtt ggagattcta ttatggacta
    11761 tggtatgctt aatatctcaa ataaatccta tgtattgaag catggtgata taaataaaaa
    11821 tgaaatagaa aattcattta tatcaagttc tttcaaagga atgagtggaa ctgaagagat
    11881 attatctaac attttagacg agaactgcct acttaatata taatgactga tatataaata
    11941 ataaactaga aaaatctgaa ataaattata gtaaagttta tcatactaat ttatttcaga
    12001 tttttatcaa tttatattta agtaagaact caatatgtta aaatgaaggt ggagtcattg
    12061 cccagactga gatacatacc tcatcatcat aattttcata cacatgtggt atagtactgt
    12121 taaaataaaa actatctcct tcctccaata gatattcctt atttccaagt gttatcttca
    12181 ttttaccttt tattatatag ccacattctt ctccttcgtg tgtcacaaat tcactgtttt
    12241 tttgtgtatg accatctagc gtaacaagta taaattctat ttttttccca gataaattag
    12301 gaacaagaag ttcataaaat tttgtagaat cattagttac tatttttttt cgattatttt
    12361 tctttactac tatatcagag tcctctcttc caatctcttc aaaaaaataa cctatattta
    12421 tatcaagagc ttttgatatt ttccacattg ctacaataga tggaacaacc atattacgct
    12481 caatttgact tattaagcct gtacttaaat ctgaattttt tgctaaagta gagatactta
    12541 tatttttaga ttttctaaga gatctaattt tgcttccgac atctatattc attagataca
    12601 aaccccctca gacatgatta taacatataa tttataaaga ttaataaaat aaaaaaaagt
    12661 tttataatgc atgtattttt tatgttatat tgaattttct gaaaagatgc attataatag
    12721 tattaatatt caatatattg aatattgata atgatatttt aatttaatga aatggaggta
    12781 attaagatga agagttttgt aagtcctgat aatatttggg tattatgggc agttgtaact
    12841 ggatgggcag catttagtat ttacttggaa caaaagtaca attgggcatc aaaagtgtcg
    12901 ggagcaataa tagctcttgt aggagcaatg ttattatcaa atctaaatat tataccagta
    12961 gagtctgttg tatatgacca agtatgggga tatgttgttc ctttagccat agctttattg
    13021 ttatatcaat gtaatattaa aaagatatgg aaagaaagtg gaagattgct cattatattt
    13081 cttgtaggtt ctgtaggaac tgtacttgga gctatgattg gatttttagc attaaaaaat
    13141 gtagtaccag atttaaatat tgttgcagct atgatgacag gttcttatat aggtggaaat
    13201 gtaaactttg ctgctatgtc aggggcgttt gatgcgccag gagaattagt atcagcaaca
    13261 gttgtagcag ataatctttt aatggcatta tatttctttg tcttaatagc tataccatca
    13321 attggatttt ttagaaaaca ttttaaacat ccacatgtag atgaaatgga aagtataggt
    13381 ataactgaag gagaaacagt agcagcaaat tattggggaa gaaaagaaat atctttaaaa
    13441 gatatagcaa tagccattgg tagcgccttt ataattgtcg cagtatctgt agaactatca
    13501 acttggttta aagctataat accattatca aatccttttt tagcaatgtt aaatacttta
    13561 tttggaaatc aatatctaat aataactact ttgactatgt tgtttgccac atttatgcct
    13621 aatttctttg gtgatataaa aggagcacag gaattgggta catttttaat atatatattc
    13681 tttgttgtag taggtgttcc tgcttcaata acattaataa ttcaaaaatc accattacta
    13741 cttttatact gcggaataat ggtactaata aacatgctag ttacatttat agttgcaaaa
    13801 atatttaagt ttagtttaga agaagctatt cttgcatcta atgcaaatat tggtggtcct
    13861 acaacagcag cagctatggc aatatcaaaa ggatggtcta agctagtagg gccaatactt
    13921 atagttggaa catttggata catagttggt aattattttg gtcttttagt aggaaatatt
    13981 ttgatataat tttacgtgaa aagaagttgt tttttagaaa gatttttatt taattggagg
    14041 ttgttatgat tttcgatgga cattcagata cttgggcaga tgtaacaata agaaggctta
    14101 acggggaaaa agatgtattt agaagaatcc atcttgataa ttttaaaaga gcaaatatag
    14161 aaggagctat ttttgtaata tggatagacc caccatatga taaagagcct gaaaaaagag
    14221 ttaaacaaat tataagctct ataaaagatg aaattgaaga tatgaaagat gttgtaaaca
    14281 tagtaaaaaa atattcagat atagataaag gtataagtga aaacaaaata aatattttaa
    14341 ttggtcttga aggattaagc catattggaa gaaatataga tttactagat tatttttata
    14401 aagaagttgg tgcaagacat gcatcattaa cttggaatga acagaatgaa cttgctacag
    14461 gatgtttagg ggatgaaaat cgtggtttaa ctgaaatagg taaaaaagca gtcaataaaa
    14521 tggaaaaatt gggcatgata gtagatgtgt cacatttaaa tgaaaaatca ttttgggatt
    14581 taatgtctgt tgcaagtaag ccagttatag cttctcattc taatgctagg agtttatgtg
    14641 atgctaaaag aaatcttaca gataatcaat taaaagaaat ttcaaataca ggtggattag
    14701 ttggaataaa tagttttaga ggatttacta atagtaatcc taagaagcaa gatttaatac
    14761 atttggttga ccatatagac tatatggtaa atcttatagg tatagaacat gtatcttttg
    14821 gttttgattt tagtgattac ttagggaaag agactatagg ttcttttact ggaaatgcta
    14881 gtgtgccaag tgtagatggt cttgaaagca cacatacagc acagaacata gtagtagagc
    14941 ttgaaaatag aggttataaa aagaaagata tagagttaat ctctagtggt aatttttata
    15001 ggctctttaa agatatacta aaataatata ataaagaact taaatttata aaatagtata
    15061 tttatacaat aatatctaca tatttaaatt ggcgttcata atttattaaa ttaaatttta
    15121 atgttactag aagtttcaat tctagtaaca ttttttatgt taaaaataga tatagatata
    15181 tattgacaat gataattatt attgataaac tataaaagtg aaaatgataa taattttcat
    15241 tttaattaat aaaagatttt taaggaggga gaatgaagaa aaggtattta gtcataggaa
    15301 taataatttt gtcttatttt tctttattta taggagctga agatataaat ataatgcata
    15361 tatttgcaaa aaatcagcat aagcttatga tatttatcat gagtagggta ccaagattaa
    15421 taagtatatt gattgctggt gtaggaatga gcatagcagg tcttataatg caacagataa
    15481 gtaagaataa atttgtatca cctacaacag gtgctacaat agatgcagct caatttggga
    15541 tagtaatatg tatgctttta gtaccaactg cctctatttt tacaaagaca ataatagcat
    15601 ttgtattttc tcttgtaggg acttttacgt ttatgaaaat aatagggaag ttacaattta
    15661 aaaatataat ctttgtacca ttagtaggta taatgtttgg aaatataata ggttctatga
    15721 cggattttat cgcgtataaa tacgatttga gtcaaaatgt tagttcttgg atgcaaggtg
    15781 acttttctat gatacttaaa ggaaattatg agatattata tatcacaata ccccttataa
    15841 tattagcata tatttatgca aataaattta ctgttgttgg tatgggtatg gattttgcga
    15901 caaacttagg tcttagttac aaaagaattg tcaatatagg tctgataata gtagcacttg
    15961 ttacagtatg tgtagtcgtt acagctggta atatcccttt tataggtcta atagttccca
    16021 atatcgtatc cttgtatatg ggtgataata taagagcaag catatggtat actggtttac
    16081 ttggagcggt atttgtactt atatgtgata tttttggaag aattattatt tatccatatg
    16141 aaatatctat aggacttaca gttggagtta taggaagtat actattcctt tatttaatat
    16201 taaggaggaa tgtgaatgaa gcttagtctc aatcaaaaaa tttactttgt aagtatatta
    16261 ctattcatat caatagcact ttttataatt tatggaatag acatgaatca tttagaatat
    16321 gcattatcac aaagaatacc aaaaatattt gctatggtat taggtggagg atgtatagca
    16381 tttactactg tagtgtttca aacaattaca aacaatcaaa tattaacacc aagtgtacta
    16441 ggtttagatt cattgtatgt aatgattcaa actataatag tattcatatt tggttcaagc
    16501 agtagtctga ttataaatga aaattataat tttattatta atgtagctat gatgattgga
    16561 gcttcattgt tattatataa gatgttattt gaaaaaaata aaaataatat tttcttttta
    16621 atcctagtag gtatgatatt tggaacctta tttaaaagtg caacaacatt tattcaagta
    16681 atgatagacc cgaatgaatt tttagcttta caaactagta taatggcaag tttaaacaat
    16741 ataaacacta atgtattgtt aatagcattt ataataatta ttgccataat accttttata
    16801 tatgatgaaa taaagtattt agatgtattg tcgttaggta aagaacaagc aattaattta
    16861 ggtgtagatt ttgataaggc agttaaaaaa atgataatat taatagcaat tttagtctca
    16921 atatcaacag cacttattgg acctatgaca tttttaggac ttttacttgc taatataaca
    16981 agagaaattt ttaaaacata taagcataca tacttgattt cgggttctat gttgataggg
    17041 atgattacat tgataatagg tcaatttttt atacaacatg tatttaagtt tgatacgact
    17101 ttaagtgtag taataaattt tataggagga atttacttta ttcaattact attaaagggg
    17161 gcaaatagat gatagagata aaaaatatat ttaaaagata taagaataaa aatgttgtag
    17221 atgatgtctc ttttagtata gaaaagggaa agataacatc ttttatagga ccaaatggag
    17281 ctggaaaaag tacagtacta tcaatagtta caaggcttat aggaggtgat ggcggagaag
    17341 taataataga aggaaaaagc ttgactaatt atagcaataa agaattagca aaaaaaatag
    17401 ctattcttaa acaatctaac aatataactt taaagcttac aataagagaa ttagttggat
    17461 ttggaagatt tccatatagt gaaggtaatc taactaaaga ggatgaaaat tatattgatg
    17521 aagcaataga atatatgaag ttgactgata tacaacataa gtatttagat gaattgagtg
    17581 gaggtcaaag acaaagagct tatatagcaa tggtaatagc tcaagataca gagtacatat
    17641 tacttgatga accactgaac aatttggata tgaaccattc cgtacagatg atgaaggtac
    17701 ttagaagtct ttgtgatgag ttagataaga ctatagtatt agtaatgcat gatataaatt
    17761 ttgcatcatg ttattcagat aatatagttg cattaaaaaa tggaaaagtt gaaaaagtag
    17821 gtcgtacaga tgagatagta aatgaaaaag ttttagaaga tatttatgag atgaatttta
    17881 acattaagaa tataaatgga aatcgaatct gtatttattt ttaaaatatt gtatatgaaa
    17941 ataagcttgc tatatgattt tttataaagt ttttaaatta aaataaagat taataggaga
    18001 gaggaaaata ttgatatgaa taaaaaagca gcaatagtag cagcagtagc tattatagga
    18061 ttagtgacag tgtttgcatt aggtggaagt aaaaagaatg aatcaaaaac atcagaaaat
    18121 tcaaataata ccataaagat aactcataac ttgggagaaa cagatgtaaa attaaatcct
    18181 aaaaaagtag tagtatttga ttattcggct ttagatacaa tggatgcatt aggtgtagct
    18241 gagaatttag taggacttcc aaaaacgagt ttaccagcat ctttagaaaa atataaggat
    18301 aagaagtatg cagatttagg aggtttaaaa gaaccagatt tagaaggtat aaagtcggca
    18361 aatccagatt taattataat aaatggaaga caagaagatt tttatgaaca attgtcaaaa
    18421 atagcaccaa caataagtac aagtaaagat gataagaagt acttagaatc tgttaaaaat
    18481 aatatagaca aaatagcaaa aatatttgga gtagaagaaa aagcaaatca agaatttagt
    18541 aaaattgaaa agaaaataga aactttgaat aaaaaagtaa cagataaaaa tttaaatgct
    18601 ttaactataa tggtaaatga aggtaattta agtgtatttg gtgaagaatc aagatttagt
    18661 atattataca atagttttgg atttgaaaat aaagataaaa atataaaaga atcaagtcat
    18721 ggacaaaata ttacttttga atatatagct aaacaaaatc cagaagttat gttcgttata
    18781 gatagaggaa tagctactgg tagtgatgta aaagaaagct caactgcaaa atctgttttg
    18841 aacaatgata taataaaatc tatggatgca tataagaatg ataatataat atatttagat
    18901 tcaccaacat ggtatgtaaa tgatggtggt ctaacatctt taaataaaat gatagatgat
    18961 gcttcaaaag cggtaaatta ataatccgat ataatcaaaa tgtatttttt gtaaaatgat
    19021 tatcaaataa actaggagtt tttaagaact cctagtttat tttttgcata ataaagatat
    19081 agtttaaaca ataatattat ttataataag ataaatctat tcaaaatcta ataaaatatg
    19141 aag
//
LOCUS       DAELSY010000073        18629 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.073, whole genome shotgun sequence.
ACCESSION   DAELSY010000073 DAELSY010000000
VERSION     DAELSY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18629
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.073"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            28..1065
                     /locus_tag="KPG09_002227"
     CDS             28..1065
                     /locus_tag="KPG09_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBG0966836.1"
                     /translation="MSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEEKREPVKKDIT
                     ISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDLTVVNLEGPLT
                     TASNAKEKKFAFKGKPGYVNILKSGSIEAVSIANNHSEDYFEEGMKDTQFILDENKIN
                     YFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKSDVVVVYFHWG
                     IELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYSLGNFCFGGNK
                     NPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSSSRNNYQPKVLDGSEKDRVLN
                     KLEKISKDLNK"
     gene            1235..3136
                     /gene="cooS"
                     /locus_tag="KPG09_002228"
     CDS             1235..3136
                     /gene="cooS"
                     /locus_tag="KPG09_002228"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG0966837.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            3129..3602
                     /locus_tag="KPG09_002229"
     CDS             3129..3602
                     /locus_tag="KPG09_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0966838.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            3577..4803
                     /locus_tag="KPG09_002230"
     CDS             3577..4803
                     /locus_tag="KPG09_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0966839.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDVCY
                     ADFFNIKENGEFCYSV"
     gene            complement(4942..6129)
                     /locus_tag="KPG09_002231"
     CDS             complement(4942..6129)
                     /locus_tag="KPG09_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0966840.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKSFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            6402..7226
                     /locus_tag="KPG09_002232"
     CDS             6402..7226
                     /locus_tag="KPG09_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBG0966841.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            7355..8620
                     /gene="gluD"
                     /locus_tag="KPG09_002233"
     CDS             7355..8620
                     /gene="gluD"
                     /locus_tag="KPG09_002233"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBG0966842.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            8813..9469
                     /locus_tag="KPG09_002234"
     CDS             8813..9469
                     /locus_tag="KPG09_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG0966843.1"
                     /translation="MYFLPPHDYKLEELDWIFGHELMYFKSKDLHLKYLVLFLKIVYW
                     FNPFIYIMEKYMDLDCELYCDERVLKNCTVQQKQEYALTIVNSMKRGINFSNKFVTGL
                     NKQSDIRKRVMNMFSEKHKNGILMALIISLFSSITFFKFDSTSLIEENDFLERSRYAF
                     EGRQEDMFVTIDSTYADAPIKLRKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK
                     "
     gene            9800..10933
                     /locus_tag="KPG09_002235"
     CDS             9800..10933
                     /locus_tag="KPG09_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBG0966844.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGFEDLRTVSSIRFSVIGF
                     TYADAPKNMREEYKINCESLGLIPKDSDKIEVGVEYYDSLLND"
     gene            11860..12882
                     /locus_tag="KPG09_002236"
     CDS             11860..12882
                     /locus_tag="KPG09_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0966845.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            13178..14098
                     /gene="pyrB"
                     /locus_tag="KPG09_002237"
     CDS             13178..14098
                     /gene="pyrB"
                     /locus_tag="KPG09_002237"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBG0966846.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            14137..14829
                     /locus_tag="KPG09_002238"
     CDS             14137..14829
                     /locus_tag="KPG09_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBG0966847.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            14822..15724
                     /locus_tag="KPG09_002239"
     CDS             14822..15724
                     /locus_tag="KPG09_002239"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG0966848.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            15809..16393
                     /locus_tag="KPG09_002240"
     CDS             15809..16393
                     /locus_tag="KPG09_002240"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBG0966849.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            16608..17534
                     /locus_tag="KPG09_002241"
     CDS             16608..17534
                     /locus_tag="KPG09_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HBG0966850.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFNYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIGKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYILSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVENSLHRILEHSKEGRTSMLQDVEAHRPTEVDMF
                     SKNICKLGKKYSIPTPINQTLFYMIKVIESRF"
     gene            complement(17586..18476)
                     /locus_tag="KPG09_002242"
     CDS             complement(17586..18476)
                     /locus_tag="KPG09_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0966851.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSQKYPDIYVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYNSHLLKDFSFDKLQDQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
BASE COUNT         7319 a         1990 c         3309 g         6011 t
ORIGIN      
        1 aaaaataaat tagtttacat atgtattatg agtctattgc tatttgtagt aggatgttct
       61 aataatccaa ataaggaaag taccattaaa aaagactcta agaataacca aagccaagtt
      121 aaagaagaaa aaagagaacc tgtgaaaaaa gatataacta taagtttcgc tggtgatgta
      181 actatgggga actataaagg ttcatcatat tatggcagtt ttgaccatga atttaaaaga
      241 caaggagaag attacaatta ttttcttaaa aatgtaaaac ctgtatttga aaaagatgac
      301 ttaacagtgg ttaatttaga aggtccactt actacagcgt caaatgcaaa agaaaaaaaa
      361 tttgcattta aaggaaagcc tggatatgta aatattttaa aatctggaag tatagaggca
      421 gtaagtattg caaataatca ttcagaagat tattttgaag aaggaatgaa agatacacag
      481 tttatattgg atgagaataa aataaattat tttggattag ggaaagatgc tgttgtagat
      541 gtcaaaggaa ttaaagtggg tctattaggg tataatggat tatcaactga atataatgaa
      601 aaaaatttaa aacaaatgga agatgatatt aagtccttaa aaaagaaatc ggatgttgtt
      661 gtagtgtact ttcattgggg gatagaatta gattattatc cagataaaaa acaaaaagat
      721 tttgctcatt atgctataga caagggagca gatttagtta tggggtctca cccacatgtt
      781 attcaaggaa tagaaaagta taaaaataag tatatagctt atagtctagg gaatttttgc
      841 tttggaggaa ataagaatcc ttctgataca gatagttata tatatcaaca aacctttact
      901 ttttcagata ataaattaac ttcaattaaa gagccaaata taataccaac ctcaataaca
      961 tctagtagct ccagaaataa ttatcaacca aaagtattgg atggaagtga gaaggataga
     1021 gttttaaata aacttgaaaa aataagcaaa gatttaaata aataaaatgt aaaaaaaaat
     1081 aaatatatat atttatttta ataatttata ttttgtttag ggtatggtaa atcgtggtat
     1141 taaataagta gattttaata gttaaattat agtcaattat aataaagcta tttaatctac
     1201 ttttttaata taaaaatata agggggaatt tgtaatggct tgtaacactt gtaaaatgtg
     1261 cgaaagtgca gacaagaaat tagaaagttt tgtagcttca aaggatgttg aaacagcatt
     1321 tcatagaaca gaagatcaga aagttaagtg tggttttgga cttcaggggg tttgttgtag
     1381 actctgttca aatggaccat gtagagtaac tcctaaatca ccaagaggta tatgtggagc
     1441 agatgcagac actatagtag caagaaactt tttaagagca gtagcatcag gagcagcttg
     1501 ttatttacat gtggtagaaa atacagctaa gaatttaaaa aatgttggaa taactaaagg
     1561 tgttgttaaa ggagaaaaaa cattaaatca attagcagag atgtttggaa tagaaagtga
     1621 tgaaaagtat gataaatgta taaaggttgc agacaaagtt atcaacgatt tgtataaatc
     1681 aagagatgat aaaatggaat tagtagaaaa aatagcttat gcacctagag tgaaaaagtg
     1741 gaaagagtta ggtataatgc ctggtggtgc aaagtcagaa gtttttgatg caatagtaaa
     1801 atcatcaaca aatttaaata gtgatccagt agacatgcta gttaattgtt tgaacttagg
     1861 tatatcaaca ggattatatg gtcttacact aactaatctt ttaaatgatg ttatgttggg
     1921 agaaccagtt ataagaatgg ctcctgtagg atttaatgta atagacccag actacataaa
     1981 tataatgata acaggccatc aacattcaac atttgcaaat ttccaagata gattaaaaga
     2041 tgaagatgtt ataaaattag ctcaatctgt tggtgcaaaa ggctttaagc tagtaggatg
     2101 tacatgtgtt ggtcaagatt tacaattaag aggagaacat tatcaagaag tatttgctgg
     2161 acatgcaggt aacaatttta caagtgaggc tgttttatca actggagcaa tagatatagt
     2221 tctttcagaa tttaactgta ctataccagg gcttgaacca atagcagata aatataaagt
     2281 aaaaatggtt tgtttagatg atgttgctaa aaaagcgaat gcagaataca taggtttaga
     2341 cagaagtaaa ttagatgaac ttagtaacac tttaatagaa aaagcattgg agtcttataa
     2401 ggagagaaga ggaagtattg agatagatat accaaaagac catggatttg aacaatcttt
     2461 aactggtgta agtgaaaaga acctgaaagc gtttttggga gattcatgga aacctcttat
     2521 taatttaatt gcagaaggaa aaattaaagg tgttgcagct gttgtaggat gttcaaatat
     2581 gacagcagga ggacatgatg taaatactgt tgaacttact aaagaattga ttaaaaaaga
     2641 tataatagta ctatctgcag gatgttctac tggaggatta gaaaatgttg gtcttatgtc
     2701 acctggagca gaagaattag caggagaaaa cttaaaagaa gtatgtaaga ctttaggaat
     2761 accaccagta ttaaactttg gaccatgtct agctatagga agactagaaa tagtagcaac
     2821 agaattagca gctgaacttg gaattgacct accacaatta ccattagtgc tttcagctcc
     2881 acaatggttg gaagaacaag cattagctga tggagcgttt ggtcttgccc ttggcttacc
     2941 tcttcattta gctttacctc catttataac aggaggaaaa ttggtgacag aagtattaac
     3001 agaaaaatta aaagacttaa caggaggaca tgttatagtg aatccagacc ctaaatcttc
     3061 agctaatcaa ttagaagaaa taataataga tagaagaaaa aatttagggt taaaggatgt
     3121 ggaatgcaat gcatagaatt tttataaata aagatttatg tacaggatgt aagagctgtg
     3181 tactagcatg tatgctaaaa cacaataaag attatgacat gtacactctt gatttagaaa
     3241 atatagataa tgatagtaga gggcatatag aattagatag taaacataat aatccagttc
     3301 caattctttg tagacactgt gatgaacctg aatgtgtatt agcatgtatg agtggtgcaa
     3361 tgcataaaga tagtgaaagt ggaatcgttt catatgatga agagaaatgt ggttcatgtt
     3421 atatgtgtgt aatgtcttgt ccatatggat tattaaagcc agatgataga agcaagcaaa
     3481 acatacttaa atgtgattta tgtaaagatg aggagtatcc tagatgtgta gctaattgcc
     3541 caagtggagc aattgaatta cagaaggagg aaaatgatga attatgtagt gttaggggct
     3601 agtgcagctg gtataaatgc agtaaaaaca ttaagagaat tagataaaga ttcaaatata
     3661 gtagtaattt ctaaagatga aaacgtatat tcaagatgta tgttgcatca cgttatatct
     3721 gaacacagaa ctttaaaaca aataaatttt gttgatgaag attttatgga gcaaaataat
     3781 gtaaaatgga ttgcaggtaa gactgttaaa ggtatagaca taaataaaaa ggtagttcaa
     3841 acagaagaca ttactgtaaa ttatgataaa cttttaatag ctacaggtgc atcttctgct
     3901 attcctccta taaaaaattt aagagatgga aattttgtat attctgtgag aaacatagat
     3961 gatatttata aaataaaaga aaaagcacaa aattctaaaa atgtagttat aataggtgct
     4021 ggtttagttg gtatagatgc attagtggga ttatttaaat atgagcagtt aaatatttct
     4081 gtagctttta tggaaaagta cattttagat agacaattgg atgaatatac agcatcagtg
     4141 tatgaaaata agttcaaaga aaaaggtgta aaattttacc caagtgcatc aatacaagaa
     4201 attgtattgg ataacagtaa aaatgttaca ggtgttgcat tttcaaatgg tgaagtttta
     4261 gatgcagaca tggtaattgt agctacaggg gttaaaccaa atgctgactt tttagatggg
     4321 acaggaatag aatatgatag aggtataata atagatgata tgtgccaaac aacacaaaaa
     4381 gacatatatg cagcaggaga tgttgtaggt aaaaatgcta tttggcctct ggcagtaaag
     4441 caaggaattg tagctgccta taatatggtt ggaaaagata agaagataga agatgaattt
     4501 gcttttaaaa atagtatgaa tttcatggat ataccaacta tttctatagg aatgaataca
     4561 cctgtagatg atagctataa agtattaacc agacatggaa ttaatgatta taagaagttt
     4621 gtttttaaag ataatgtaat ctacggagca gttatacaag gagatatttc atatgtaggt
     4681 gtattaactt atttaataaa aaataaagtt gaaatatatg atttagaaaa taggatattt
     4741 gatgtatgtt atgcagactt ttttaatata aaagaaaatg gagaattttg ttatagtgta
     4801 tagtatatac gtggagtaag gtttataaat acagtaaatc aataaataga gttattctaa
     4861 aataagaaaa atgacaaatc ttatttagaa atagctctat tttttgtaat taaatttaaa
     4921 ttatattcaa tttgtaaaat attattttga acaattatac atatactctc tagttagagg
     4981 taaatcattt tttatacctt tagtgaaaag tatttgatgt atatctataa taccagtgtt
     5041 gaaagcagca gcacaagaac aaaggtataa atcccacatt cttataaact tatcatcaaa
     5101 catttcttta atctttggaa gattctcaga gaaacttttt tgccagtata gtaaggtttt
     5161 tacataatgt aatcttaaac tttctacgtc tatagtatga tatttgtaat cactacaaat
     5221 atgtatcatt tctcttaggc taggaataac tcctcctggg aaaatatatt tttttatcca
     5281 agggtctcca taagattcct gcaatccact tatatagtga agtaaaaaca atccaccatc
     5341 ttttaaaaca gcatttacgt ttttaatgaa aagttcatag ttttctctac caacatgttc
     5401 aagcattcca acgcttacaa ctctatcaaa cataaggtct gattttttta attctctata
     5461 gtccataagt cttacctcaa gatagtcttg aaggttctct tctttaattc ttgcgttaaa
     5521 ttccttaaat tgctcttcgc taagtgtaat tccaacgccg tgaattttat atttttttgc
     5581 agcttctatt agtaaaaatc cccaaccaca accaatatcg agaaggctca taccttcctc
     5641 taaatttagt ttagttaata tgtggtgaac tttattgact tgagcatcat aaagagtatc
     5701 attatcattt ctaaagtatg cacaagaata actaagtgtc ttatctaacc ataagctata
     5761 aaaatcgttg ccaatgtcat agtgggcatg tatttctttc ttctgatgtc tcattgaatt
     5821 tgatgagtgc aatagatgtt ttacagctga attatcaata ttgaatttgt ctatttgttt
     5881 taaaagtaga tttaaagtta agaataaatc tccctcaact tcaatgtctc tatttatgta
     5941 ggcttctcca agtgctaaag atgtactttt taatagttca gacttgctaa tatcattatt
     6001 tactataact ttgaactgag gggagccctc tccaattaga aattcttcct catctttaag
     6061 cctaactaga aatggtgttt caccaaattt ctctaaatag tcttttaaaa aattttttgt
     6121 aatattcata ttaccagctc cttctaatgt aaatccagta agtttccctg cttaaaatat
     6181 attatagtcc tttttttaaa aaagtaaaat tttttcaata aattttttat ttaaaggaaa
     6241 tttatttttt ataaagaaat ttcatgttgt agtatatttt ttgcttataa attttggtac
     6301 tggatataat attaaactgt taatgatata ataaatttaa tctataaagt aaataaaatt
     6361 ttgtaataaa tagttaaagc tattttataa ggagatatat gatggaagta tatgctttgg
     6421 ttgggtcaag tggcacaggg aagagcttta aggctttaga gttcgcatat gaaaatgata
     6481 tagaatatat aatcgatgat gggattttaa tatacaaaaa caaggtttta gctgggatat
     6541 cggcaaaaca agcaaatact actatagaag cagttaaaag ggctattttt tataatctgg
     6601 agcatagaca agaagttaga gaagcaatta aaaaagaaaa tataagaaaa attcttatta
     6661 ttgggacatc aaaaaaaatg gttaatcaaa ttgtagagag attgtcaatt ggaagggtct
     6721 gtaagtttat aaatatacaa gatataagta ctaaatatga aatagaaata gcaaaacaag
     6781 ctagaaaaga aggaaatcat gtaattcctg ttccagccgt tgagataaaa agtatggcaa
     6841 gtggtctcag tataaattct ttgaagagac tttttagaaa aggaaataat aggaatatgg
     6901 tagttttaga aaaaacaatt ataagaccaa cttttagtta tataggaaag ttttatatta
     6961 gtgctgatgt aattaaacag attattgaat atgaagtata caactttgga agcattgata
     7021 gaataaataa aatcaatata gagaattgca ataattttat gaatattttt gtaagtataa
     7081 atatcaatga tttagaaact ataaaaaaaa ttgaagacat acaacagaca ataaaaaagt
     7141 ctattgagaa aatgacttta gttaatgtac aaaaaataga tattaacatt cataaaatta
     7201 aaaaaataaa cttcctccaa tgttaaaaaa taaactgaga aaatgatata ctaatttgca
     7261 ataaagtgtg ctataattaa actgtaaatt taggaaatgg ttttttctaa atggagtata
     7321 cataattata acgtatttat aagggggctt tcaaatgtca ggaaaagatg taaatgtctt
     7381 cgagatggcg caatctcaag taaaaaatgc atgtgataaa ttaggtatgg aaccagcagt
     7441 ttatgaatta ttaaaagaac ctatgagagt tatagaggtt tcaattccag taaaaatgga
     7501 tgatggttct ataaaaactt ttaaaggatt tagatcacaa cataatgatg cagtagggcc
     7561 aacaaaaggt ggaataagat tccatcaaaa tgtttcaaga gacgaagtaa aagctttatc
     7621 tatatggatg actttcaagt gttctgtaac aggtatacca tatggtggag gtaaaggtgg
     7681 aataatagta gatccatcaa ctttatctca aggtgaatta gaaagattaa gtagaggata
     7741 catagatgga atatataaat taataggtga aaaagttgac gttcctgcac cagacgtaaa
     7801 tactaatggt caaataatgt cttggatggt tgatgagtac aataaattaa ctggacaaag
     7861 ttctataggt gttataactg gtaagccagt tgaatttggt ggttctttag gaagaacagc
     7921 tgctactgga tttggtgttg ctgttactgc aagagaagct gctgctaaat taggaataga
     7981 catgaaaaaa gctaaaatag ctgttcaagg tataggaaac gttggttctt atacagttct
     8041 taactgtgaa aaacttggtg gtactgttgt agctatggct gaatggtgta aatcagaagg
     8101 ttcttatgct atatacaatg aaaatggttt agatggtcaa gctatgttag attatatgaa
     8161 agaacatggt aacttattaa acttcccagg agctaagaga atatctttag aagagttctg
     8221 ggcttcagat gttgatatag taataccagc tgcattagaa aactctataa ctaaagaagt
     8281 tgctgaatct ataaaagcta aattagtttg tgaggctgct aatggaccaa ctactccaga
     8341 ggctgacgaa gtatttgctg aaagaggaat agttcttact ccagatatat taactaatgc
     8401 tggtggagtt acagtttctt acttcgagtg ggtacaaaac ttatatggat actactggtc
     8461 agaagaagaa gtagagcaaa aagaagaaat agctatggtt aaagcatttg aatctatttg
     8521 gaaaatcaaa gaagagtaca atgttacaat gagagaagct gcttacatgc attcaattaa
     8581 gaaagttgct gaagctatga aattaagagg atggtactaa gatttactct taataaataa
     8641 taggatataa ttatttaaga tataaagggg ctgtacatag tacagccctt aaattatgtg
     8701 gcttacaatg aagtaagcta tttttgtttt aacttataaa taacttgata aattatgtaa
     8761 tttagaatgc ataaataaga tgggtgaaaa ttaatggttg atttaatttt gaatgtattt
     8821 tttaccacca catgattaca aattagagga gttagattgg atttttggac atgaacttat
     8881 gtattttaaa agcaaagacc ttcatttaaa gtatctagta ttatttttaa aaatagtata
     8941 ttggtttaat ccatttatat atataatgga aaaatatatg gatttggatt gtgaacttta
     9001 ttgtgatgag agagttttaa aaaattgtac tgtacaacaa aagcaagaat atgctctaac
     9061 tattgtaaat tcaatgaaaa gaggtataaa tttttcaaat aaatttgtaa ctggattaaa
     9121 taaacaatct gatattagaa aaagagtaat gaacatgttc agtgaaaaac ataaaaatgg
     9181 tatcttaatg gctttgatta taagcttgtt ttcttctatt acatttttta aattcgattc
     9241 aactagtctt attgaagaga atgatttctt agagaggtca agatatgcgt ttgaaggaag
     9301 gcaagaagac atgtttgtaa caatagattc tacatatgca gatgcaccta taaaacttag
     9361 aaaagagcat gaagaaaact gtagagtcaa tggaatagta ccaaaagatt cagattcaat
     9421 agaaattatt cctagatttt ataaagattg tatggattta aaaaaataag ttagacttaa
     9481 tattgatgtt gatattatga attttttata tttaagcaat tgaaaatttt attatagatt
     9541 caattttata tgtgtttaag tatgaaattt atagatagtt gaaataatta aatatgattg
     9601 atgcatagga atcattacta agctataatt aattataggg gagtttaaat aaattatgag
     9661 ttacacaatt ataaaatgat aaaaaatagt tatctgttac aaaaaatgta tctttttaat
     9721 taataaagat aatatgaaaa cttaaaaggc aaataaactt aaacataaaa tactcttaaa
     9781 taagatagga gaaaaatgta tgtctaactt tattctaaat gggattttta gaacagttat
     9841 agtaagtagt ctaagcattc ttctgatatt gattttcaag aaaaatgtat ttaaaagatt
     9901 tagcaaaaaa tttaattatt atatatggat gatagttgtt attaaattat ttttaccatt
     9961 tacatattat acttttacaa taaatatctt gagaagtaaa aaacacatta atattaataa
    10021 cataaattta gagggattta ataatgtttc aactataagt aataatatta tattgtacac
    10081 ttggattata actgtagtag tgtatttaat ttacactata tttaaatata taaaacttaa
    10141 aaatttaata aatgacttat cttatgatgt tgatgataga gagattgtaa atctttataa
    10201 aaatatatta gaagaattta atataacaaa agatataaaa ctcaaatact cttatgaagt
    10261 tgaaacacca gcatttttta attcatgtgt acttttgcca ccacatgagt ataaattgaa
    10321 agagttagac tggattttta gacatgagct tatgcatttt aagagtaggg acctttattt
    10381 aaaatatgta atcctgtttt taaaaacagt atattggttt aatccattta tatatataat
    10441 ggataaacat atagatttag attgtgaact ttattgtgat gaaagagttt taaaaaatcg
    10501 aaattcagag gaaaaaaagg attatgcttt aacaattcta aatgcaatga gaaaaggttc
    10561 aaatacttca aataaattta tagctggact ccataagcaa tctgatatta aaaagagagt
    10621 tatgaatatg tttaatgaaa agtacaagaa tggtatatta atggcattaa ctttatgttt
    10681 attatcttct attacttttt tgaaggttaa ttcaacgagt attgttaatc ttaatgtatt
    10741 acctaaagga tttgaagact tacgaactgt tagcagtata cgtttttcag ttataggatt
    10801 tacatatgca gatgctccta aaaatatgag ggaagaatat aaaataaatt gtgaatcatt
    10861 aggtttgata cctaaagact ctgataaaat agaagttggt gtagaatatt atgacagtct
    10921 attgaatgat taaataaata ttattttaac agatattaat tataaagttt tgaatagtaa
    10981 tttgttgaga aataaatata tccttataaa aatagatact tatataccaa gaataaaaaa
    11041 tagttaagat agtacacaag gatatactta taaagaactt aaataagtga aataaattat
    11101 taattataag gtttataatt ttaaaaaatt agataaattc aggtgtaaaa tactataaaa
    11161 cacaattcgg aaaagtgtat gttgaacttc atttaaaata gaattttcaa agttgttata
    11221 gtaagtagtt taagcattct tcttatatta atctttgaga ataatgtact taaaaaagtt
    11281 agtaaaaaat ttaactattg tatattaatg atagttgcta ttgatatatt atctaaaaaa
    11341 attaagtatg aaaagattac tgctaggaca atcttttcat acttaatttt ttaatattta
    11401 tattctccaa aattttcatc tttttaactt agaattatta gaatttgaga atcactatta
    11461 ggaaataggt tttgatttat gcagggttaa aaatttcaca gaatatctaa ctttatacta
    11521 aatccatcaa aaaatatacg ccatataaaa gcgcataaaa aaacacataa aacaatattt
    11581 atgcgcactt taacctcgaa tggtacaaaa ttctttactt ttacagaccc atattatata
    11641 attaagtaaa taatgtatat ttaggtgaca tattggataa gctaatttat ttattttatt
    11701 aatataatga tactataaaa gttatataaa gtggtttaca aaaaagttac agatagagct
    11761 tttattaatg tattgtgtat tagtatttta tccttaggta cacttcacct acaaaataga
    11821 atcataagat aattatggga aaattttagg aggtttatcg tgaagaaaaa gatattaata
    11881 ccagtatttg catcagttat ggcattatca gtaagttcaa ttgtaaatgc agatgaagta
    11941 aatgattcat ctcaaaataa ggatgataaa acaaatactg aattaaattt aggtgaatat
    12001 aaagaagtaa aatataaagt tgcaaaaata aaagatggag tagctataaa gataagagaa
    12061 gaaggtcaag ttcaaaacat agcttattct ggagatgaat ttacggtttt aggaactcaa
    12121 ggagaatggg ttaaggttaa agttgaagat ggcgaaggtt ggttagctac tagatatgta
    12181 gatatatctg aaggtgtagg atatactaac gctgataaag taaacttaag aaaagataag
    12241 agtgaaagtt cagaagtaat tgaagagctg gaaaaaggaa gttctttatt ggttttggag
    12301 gataatggag attggttaaa agttaaagat ggggaaacag aaggatatgt aaaatcatct
    12361 tatatatctg ataaagctcc tgtaatagaa gagccacaag taaacccaag tattgaccaa
    12421 aatgtagatt ctacaaataa taatcaacaa aacaatgcaa atactaatca acaaaataat
    12481 aattcaaatg tgccaacagc taactctaat gcagttcaag ctgtacttaa cttggcatat
    12541 tctaagcaag gttgtccata tgtttgggga gcagaaggac caaacacttt tgactgttct
    12601 ggatttacac aatatgtgta tagaaatgca gtaggaaaga atataccaag aacatctaaa
    12661 gcacaatcta aatatggaca aactgtaagt aaagcaaatt tacaaccagg agacttagta
    12721 ttcttcacaa ctaatggttc tggttctgtt agtcatgttg gaatatatgt aggtgggggt
    12781 aatatgatac attcaccatc aacaggaaaa acagttagtg taactagtat aaattcatca
    12841 tactatacag caagatttgt tacagcaaaa agaatattat aatcggtttg taatatgaca
    12901 aattgataaa tacataaata tatataaacg ctctatatta tatttaattt aatcatggaa
    12961 acaaaaaatg attatatttt aaatgtacta tagagcgttt ttgtatcact ttaaatctaa
    13021 tcaagattca agttaatatg actattaaat aggagccttt tcatatgatg attatttgcg
    13081 caatcccttg acaggatagg gtaaaggtgg taagctacta acagtacaaa aatactttta
    13141 attttagttc agagaggcta aaaaaggagg acgaaagatg ttaaaatcaa gaaatttaat
    13201 ccaaccagaa gacttttcaa tagaagaaat agatgaaata ttagaattag ctcaaaaaat
    13261 aattgacaat ccgtcaaaat attctcgtat atgtgaagga aaactattag ctacattatt
    13321 ttatgaacca tcaacaagaa caagattaag ctttgaatct gctatgaaca gattaggagg
    13381 acgtgtagta ggcttttcag agccaaattc atcctctgca tcaaaaggtg aaactctagg
    13441 agatacaatg aggatagtat ctggttatgt tgatataata gctatgagac atcctcaatc
    13501 aggtgcagct tcagaagcag ctagatatac ggaagtgcca tttataaatg caggagacgg
    13561 caaaaaccag catccaactc aaacacttac agacttactt actataaaat cattaaaagg
    13621 aactttagaa agccatacaa taggattatg tggtgattta aaatatggaa gaactgtgca
    13681 ttctttggtt aaggctatgg caagatataa aaatacaaag tttgtattta tagcgcctga
    13741 agagttaaaa atgccagatt atataaaaga agctataaag ggacatgcat attatgaaac
    13801 taataatttg gatgatgtta taggaagctt ggatgtttta tatatgacta gagttcaaca
    13861 agaaaggttt gaagataaat cagagtatga aagacttaaa aactattata ttttaaataa
    13921 agctaaatta gaaaaggcat ctaaagatat gttagtaatg catccattac caagagtaaa
    13981 tgaaatagac atagatgtag atagtgatga tagagctgta tattttaaac aagctaaata
    14041 tggaatgtat gttagaatgg ctttaataat aaaactttta ggtataaatg aagactaata
    14101 gttatcatat aatagtaaaa caaaggagat tttgggatgt ataaaattct ggaaaatata
    14161 tatataggcg aagatatgta tagaatgaaa gtaaaaggta attttgaagg taaaatggga
    14221 caattctata tgttaagagc atgggatact tatccagtac tttcaagacc tataagtata
    14281 catgatatag atgaagaagg gataactttt ctttataaag tagtaggaga aggaactcaa
    14341 attttgagta acctaaaggt aaatgataat ataaaattag aaggccctta tggtaatgga
    14401 tatgctaagg ttgatggaaa ggtagctcta gtaggaggag gaatcggtgt agctccacta
    14461 tatttggttg ctaaaaatat aaaaaattgt gatgcatatc ttgggtttag agaagatgtt
    14521 atattagaag atgaatataa gcaagtatgt aataaagtct atacaacagt aggaaatact
    14581 tttgtaacag atataattga tgttgagaaa tatgattata tattaacatg tggtccaact
    14641 ccaatgatgg aaaaattagt taagatggta gaaggtacta aaacgagaat tatggtatct
    14701 cttgaaaacc atatggcttg tggagtaggt gcctgtttag tatgtacgtg taaaactaat
    14761 ggaggaaaca aaaagacttg taaagatggt ccagtgttct ggggagagga cgtgattttt
    14821 aatgggtaat ttaagtgtga aatttggaag tgtagaattt aaaaatccag taataatggc
    14881 gtctggaact tttggatttg gaaaagaata taatgagatt tatgatatac aaaaacttgg
    14941 tggtataagt agcaagggtc ttactttaaa taaaaaacct ggtaacaatg gtatgagagt
    15001 tcatgagaca ccatccggta tgatgaatag tgttgggctt gaaaaccctg gagttcaagg
    15061 atttatagac tatgaattac ctttttttag taaactagac ttagtcagaa tagctaatgt
    15121 tggaggtggg acattagaag actacttatt aggggttcaa atgttaaatg acaagcctat
    15181 agacattatt gaattaaata tatcttgccc aaatgtcaaa gctggtggaa tggcttttgg
    15241 tataaaaaat gaagtggcaa gagaagttgt tcgtgaggta agaaacataa cgaaactgcc
    15301 tttagtaata aaattatcac ccaatgcaga agatattgta ggaatggcaa aagtttgtga
    15361 agaagaaggt gctgatggag tttcattagt taatactttt aaagcaatgg caattgatat
    15421 aaaaaataga agaccagtat ttgaaaatgt atatgctgga ttatctggac cagctataaa
    15481 acctatagct ttgagaatgg tacatgaagt gtgtaaaaat gtaaatatac ctgtaatggg
    15541 aatgggcgga ataactaaag ccacagatgc tatagagttt ataatggcag gagctacttg
    15601 tatccaagtt ggaactgcaa attttatgaa tcctagaata ggtattgaaa taatagatgg
    15661 gattaatgaa tttatggata gagaaggaat aaaaagttta gacgaaataa gaggaattat
    15721 ataaggtaga ataaatagta ttttaaagat aaaaaatata gattaaataa acaataattt
    15781 tataacatta acatggagga aaaataatat gagtaaagta gatgtagtgg atatattaaa
    15841 gaaaagtgat gcattgttgg agggacattt tttattatct tcaggaaaac acagtaatag
    15901 atacgtacaa tgtgcaaaag tattaagatt tccagaatat gctgctgagg tattaagtac
    15961 agttgttgaa caaataaaag acttagatat agacttagta gtaggaccag ctatgggtgg
    16021 agtaatagtt tcttatgagt taggaagaca attaggaaaa gaagctgtat ttactgagag
    16081 aaaagacaat acaatggagt taagaagagg atttgaagtt aaaaaaggag caaagataat
    16141 aattgctgaa gacgttgtaa ctactggtaa atcaactatg gagacaaaaa gagtattaga
    16201 agccttaggt ggagaagttg taggtgttgc atgtatagca gatagaacta atcatgatat
    16261 aggtatgcct atatatagtg ctataaaact tgatattcaa gtttatgaat ctgatgagtg
    16321 tcctttatgt aaggaaggaa aattaccagt tgttaagcct ggaagtagag agttcaagga
    16381 attagggatg taataaaaac ttaattattt atagtgttac ttaaaaaatg taaatttttt
    16441 agagtaaaaa tttgtaatat aacatataac ttaaaaataa aaagcagtta gccttataaa
    16501 tgaaggttag ctgttttttt tgttaaaaat atatttacaa aataagagat agtagcagta
    16561 caatataagt ataaggtaaa agtattatat attagggagg ggtaagtatg aaaaatcaat
    16621 taaatgaaaa tcataaaaag gttgtatttt ttggagtagg tgctgtagga gctacttttg
    16681 cagaacaatt ttttaactct aaatatgatt ttaaaattct ttgtgataat gaaagaaaga
    16741 aaagatattt agaagaagga tttataataa atggaaaaag gtatgatttt aattatgtaa
    16801 ctaaagatga gtataaacaa gaggctgatt ttataattat aggtctaaaa tataataatt
    16861 taaaagaaaa tataaaagaa ttagatggat tagttggaaa aaatacagtt ataatgtctc
    16921 tgctaaatgg agttgatagc gaagagataa taggagaaag atttggaatt ggaaaaatgg
    16981 tatattcata tgttaccaat atagatgcaa agaaaatcaa taataatatt atacacacta
    17041 ctaatgggat aattgtattt ggtaacaaag ataatagtga agatagaaaa actaatataa
    17101 taactgaagt ctttgatgat gtaaatatag aatatatttt atcaaaagat attcaacgag
    17161 atatgtggtg gaagtacatg gttaatattg gtgtaaatca aacttcagct atacttggtg
    17221 caccttatgg agtttttcag agttctgagc atttaagaga attagcaaaa tctgcaatga
    17281 gggaagttgt tgctatagca caagcaaaag acatatctct tacagaagat gatgttgaaa
    17341 attcattaca tagaatacta gaacattcaa aagaaggaag aacatcaatg cttcaagacg
    17401 tggaagctca tagacctaca gaagtagata tgttttctaa gaatatctgc aaacttggaa
    17461 aaaaatatag catacctact cctataaatc agactttatt ttatatgata aaagtaattg
    17521 aaagtagatt ttaaagggta tttgtaagaa atattgataa ttattgattt taaattttat
    17581 taatgttata tactatttaa gttactaaat atttttaact cttcaatgaa aatagttgct
    17641 gcttttgaca aagttatatc ttttggaaat atatatgaga aatgtctgtt gtagctgctt
    17701 ccaagctcta tgacagacag ctcattgttt aaaacaggta agcttgtcac tagattagaa
    17761 actattgtaa ttcctaaatt atttctaaca gattctttga cagcataatt acttccaaaa
    17821 accatcatac tttttggaat aatttcttta acacttaaaa acatatctaa atagtctctg
    17881 gttccagaac catcctccct gactatccat ttttggtctt gaagtttatc aaaactaaaa
    17941 tcttttaata aatgactatt gtatggaagt gctaaaacca tcttatcttc aaaaaaatat
    18001 tcttgaataa atgaagggga agagcaagtg ccttcaataa gaccaatgtc taaaatataa
    18061 tccttaacat gagaagatac gattgatgta ttttttataa aaacttctac atatatgtca
    18121 ggatactttt gagaaaataa tgctaaaaaa tttggtagaa tatattctcc aatagttaaa
    18181 cttgcaccaa tctttaagtg accagttata gcatctgaaa cttggcttac atcatgataa
    18241 gtaatattta aaagatttaa tatttcttta gctcttttgt acaatatttg tccactttct
    18301 gttataaaaa tggttttttg tttaatagac ctgtttataa gcacaacttt aaaataattc
    18361 tctagatttt ttatgtgttt acttacactt ggttgtgata gatttaagta ttccccagct
    18421 ttagtaaagt ttttgtattc aacaacagct acaaatgttt ttaattcttc aaacaatttt
    18481 ttgtctcctt cacctaatga aattttacat tttttatata ctttctacag ataattatac
    18541 tatatataat aaccattaaa aacattaatc attacgatat tatatattta ttttacttat
    18601 ggttattaca attatatact attgctatg
//
LOCUS       DAELSY010000074        18556 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.074, whole genome shotgun sequence.
ACCESSION   DAELSY010000074 DAELSY010000000
VERSION     DAELSY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18556
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.074"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            669..1700
                     /locus_tag="KPG09_002243"
     CDS             669..1700
                     /locus_tag="KPG09_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG0966852.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALLMKVADVVRDCL
                     DIEDNNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            1833..3131
                     /locus_tag="KPG09_002244"
     CDS             1833..3131
                     /locus_tag="KPG09_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBG0966853.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(3245..3490)
                     /locus_tag="KPG09_002245"
     CDS             complement(3245..3490)
                     /locus_tag="KPG09_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG0966854.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3638..4504
                     /locus_tag="KPG09_002246"
     CDS             3638..4504
                     /locus_tag="KPG09_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBG0966855.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(4508..5044)
                     /locus_tag="KPG09_002247"
     CDS             complement(4508..5044)
                     /locus_tag="KPG09_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBG0966856.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(5234..5437)
                     /locus_tag="KPG09_002248"
     CDS             complement(5234..5437)
                     /locus_tag="KPG09_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG0966857.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            5728..6492
                     /locus_tag="KPG09_002249"
     CDS             5728..6492
                     /locus_tag="KPG09_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0966858.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKYDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKKTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            6881..8194
                     /gene="prdC"
                     /locus_tag="KPG09_002250"
     CDS             6881..8194
                     /gene="prdC"
                     /locus_tag="KPG09_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBG0966859.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     gene            8363..8525
                     /locus_tag="KPG09_002251"
                     /pseudo
     CDS             8363..8525
                     /locus_tag="KPG09_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8995..10755
                     /gene="prdR"
                     /locus_tag="KPG09_002252"
     CDS             8995..10755
                     /gene="prdR"
                     /locus_tag="KPG09_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HBG0966860.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            11184..13064
                     /gene="prdA"
                     /locus_tag="KPG09_002253"
     CDS             11184..13064
                     /gene="prdA"
                     /locus_tag="KPG09_002253"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBG0966861.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            13145..13429
                     /locus_tag="KPG09_002254"
     CDS             13145..13429
                     /locus_tag="KPG09_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966862.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            13467..14192
                     /gene="prdB"
                     /locus_tag="KPG09_002255"
     CDS             13467..14192
                     /gene="prdB"
                     /locus_tag="KPG09_002255"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:13917..13919,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBG0966863.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            14272..15030
                     /gene="prdD"
                     /locus_tag="KPG09_002256"
     CDS             14272..15030
                     /gene="prdD"
                     /locus_tag="KPG09_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBG0966864.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            15045..15512
                     /locus_tag="KPG09_002257"
     CDS             15045..15512
                     /locus_tag="KPG09_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG0966865.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            15530..16012
                     /locus_tag="KPG09_002258"
     CDS             15530..16012
                     /locus_tag="KPG09_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG0966866.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPAVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            16032..17039
                     /locus_tag="KPG09_002259"
     CDS             16032..17039
                     /locus_tag="KPG09_002259"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBG0966867.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            17159..18040
                     /locus_tag="KPG09_002260"
     CDS             17159..18040
                     /locus_tag="KPG09_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG0966868.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         7378 a         2063 c         3369 g         5746 t
ORIGIN      
        1 gacatcttat agtttattga acctatagta atgaattaat aaatggttta attctatgaa
       61 aagatttcct tttatgcaat ttatttattg aaatatgata taatatataa aggtgtaagt
      121 attagttgtt aaaataatat ttatattagt ttttatgcat acttggtata agtttttcat
      181 atattggtta aaattgatgt tgatttatta tttttagtat tttttcacct tttgattcat
      241 actttgtaga atggtgtatt ttataaaact gattataaga caagttcatt caaattagca
      301 tttttatatc atataaataa ttgttccccc taaataagat ttagatgtat agtattaaaa
      361 tcaaaaagta taaaataaaa ttgatgctta atcatagata aatattaata aaatatacaa
      421 ggggattgtc taataaaaag ataatctcct tttttacaaa taatcctaaa ttgtgatata
      481 aatctaaaaa ttttatctta acatttaacc atttaatgat aataattgat ataataaata
      541 taaattaaat atatggtgta cttattttat gtttttagca agaaatatag catttaaaag
      601 ataaattgtg caacttaaat caaagtttaa gatgaactat taacatagat atattaaaaa
      661 cttttgtaat ggagaatata tgtgatatga ataataaagt taaggttgca gtattagata
      721 caggaataga caaagaacat gattacttaa aagataattt agttggagga attgcttttg
      781 aatgtatgca tgattatatc tttatatcag ataaatttga tgatgaagat ggtcatggaa
      841 ctgcttgtgc atcaataata aagaaagagt atgaagatgt agagttattt gtaataaaaa
      901 ttttgagaaa agatggtatt acaaatataa aagttcttga agaagcatta agataccttt
      961 tagacactaa cataagatta attaatttaa gtttatcagt cataggagta gagagtgtta
     1021 agggcttatt tgaagtttgt tacgaattgt ttagaaaagg aaaaataata gtatgttcac
     1081 tcgctaatga ttttgattta agttatccag ctatgtttaa caatgttata ggtgtaagag
     1141 gatttacttt agatggagat gattcatttt ggtacaacaa aaaatatgat gttcaatgtg
     1201 ttatggatag caattcatat ataagttgtg atataaataa ttcctataga ttaccaccaa
     1261 aatgtaatag ttatttatca gcaaagctta ctggtaaaat agctaagata ttatcagaaa
     1321 aaccaggcat aacaatatct gatttaaatg ataagcttga atcattagca actagaaatc
     1381 attgggatag ttgtgatttt gatgagtgta gtagaattgt aaactttaaa ttggatttgt
     1441 atgacaaaga aaatgctctt cttatgaaag ttgctgacgt tgttagggat tgtttagata
     1501 ttgaagataa taatgaaaaa ctctttaagg gtagcctttt taataaggag ataggtttgg
     1561 tttatgataa ttgttttaat ctgttgaaaa agctagagca tagatttgac ataaagttta
     1621 attatatgga catctcaaaa tatgacttag tttcaatata tacactgact gaattagtgg
     1681 aaagatataa aaacttataa ataaagattt gtagctaaat ttaatgtaga aggtattaaa
     1741 ttatatgtat tctttgtcgg tagattaaaa gtaaaaaata tactataatt agatagcata
     1801 agagaaaatg ccaatacagg gggagttaga taatgaaata tgaagaagca ttagagtaca
     1861 tatcacaaac taataaattc ggtataagat taggattaga aaatatcgga aagttattag
     1921 aattattagg aaatccacaa gaaactttga atataataca tgtagctgga actaatggaa
     1981 aaggttctgt gtgttctttt gtttctaata tactaagaga gtgtggatat aaagttggtt
     2041 tatatacatc tccatattta gaaactttta ctgagcgtat aagagtaaat ggacaaaata
     2101 taccacagga tgatgttgct agaataattg agttaataaa agaaaaaata gagattatgg
     2161 taaaagaagg ctatgcatat ccaacagaat ttgaagtggt tactgcaatg gcattttatt
     2221 actattcaga acaaaaggta gactttgtgg cattagaagt tggtttaggt ggcagatatg
     2281 atgctacaaa tataataaca aaatcacttg taagtgtgat agtatctata agtttggacc
     2341 atacaggtat acttggagat acaatagaaa aaatagcgta cgaaaaggct ggaataataa
     2401 aggaaaatgg agtagtacta gtatatgacc agactgatga agcgaaggat gttataaagt
     2461 cagtttgtaa agagaaaaaa gctaaatata tagaagttga ttttgatgat ataaacataa
     2521 aaaaatcaga tataaattct caaatatacg attgtactgt aatgaaagaa acttatagag
     2581 accttgaaat aaaattgata ggtgaacatc aaataaacaa ttctatcttg gcaattagtg
     2641 taataaaata tctaaaagac ataaataaat tagcaaatat aaatgaagaa tctataagaa
     2701 aaggacttat aaatactaaa tggccaggaa gaatagaaaa gataaaagaa aatccaattt
     2761 ttataataga tggtgcacat aatgaagatg gagcaaaatc tttggcaaaa gcacttgata
     2821 agaattttaa aggtagaaaa ttaaccttat taataggtat gttagaagat aaagatattg
     2881 atagtgtttt agagatattg ttgccacatt ttaataaggt aataacaaca acaccaaata
     2941 acccaagggc cataaattca gatatattaa gagaaaaagt attaaaatat gtagatgatg
     3001 taacatcaaa gcatgagata gaggatgcag taaattatac attagaaaca tcaagtgagg
     3061 atgatattat aataagtgca ggttcattat atatgattgg tacagtgaga actttagtaa
     3121 agaaattata attttataaa ataaatatac aaataaagat ataaaaaaga gttgtctaaa
     3181 aaatggacaa ctctttttat ataaagtaag tttttaataa ttttactgat gtttatataa
     3241 gtaattatat agattgacgt atagccttag ataattggtc tgggcaagaa gtaccttttc
     3301 caccacagtc aataccgttt aatttatcag caacttcaag agcattttgg ccgattatta
     3361 agcttctaag tcctgataaa ttaccattac atcctcctat aaactcagca tcaacaatca
     3421 cattattttc atcaacttca aaaatcattt ctctacaaca tacaccactt ggattaaatg
     3481 ttactttcat taaaaagacc tccattaaat attatttctt tttttaacaa taataataat
     3541 accaaatata tgcgtaataa tgcaactata aagcataata caagctaatg aaataatata
     3601 ttattgtagt atatgtattg tagagaggag gggttatatg aaaatctata tttctaacta
     3661 cttatcaaag agtattagca tagttgatta ttctacactt gaactagaaa aagaaatagt
     3721 attagaagat aatatatatc cacaccattt ttgcatagaa aaagaaaaaa atttaatata
     3781 catacctagt tcaagtaatg ggatacttta tgtcttagat ttaagtaatg ataaaataat
     3841 tgatactgtc tctataggag gaagtcttag tcaagttgta cttagtgata atgaattgtt
     3901 tgtggcaaat gaagattcta atagtatata tgtactagat aaaaacacat taaatccaat
     3961 aggaattatt ggagttgata atatgccaca tggatttgac tttgatagag aaagtaaaaa
     4021 attatatgta ccgtgtataa actcgatagt atgtatagat acaattaata aaaatataca
     4081 aaagaaaata aatatggact ttagagcttg gcatatagag cttgataaac aaaagaaaga
     4141 aatgtatata tcaacacttg atggaaagct tataataata gatgaagtaa gcatggatat
     4201 aaaaaaggta ttatatgaat ttttattacc agtagaaata agatttaatt atagtggcaa
     4261 aaaagtttat gttgctgatt tgggttataa taatgtaaga attttagatt atacaacgag
     4321 tagatacatt ggaaatattg aaataaatgg gattcctcaa ggcttagaaa tatctaaaga
     4381 tgaaaagcta ctatttgtat cagatacaca agaaaattca gtaaaagttt atgaaactgc
     4441 aaacaataaa ttaataaaag aaatcaaggt aggaaaagag cctacaacta taatttgttt
     4501 gtaggcttta atgtatgtct cttataagta aatcgataac ttcggcatac atataagatg
     4561 cattattttt ccagttattg caaataagtt ttgcttgttt ctctgatgaa acatttagat
     4621 ttaaatctat taagttagac tgattttcta tgactctaag gttgacaata aattcattat
     4681 cactttgttt tacaaaacta gactttactt gagtatctgc tagtagattt tctttgttag
     4741 aatctatata cttgtctatt ttttcagtaa cactagtagg tattcgatta aagaagtatt
     4801 ctagactttc tacgcctctt tgagtaagag cataatattc tctgtcagag tctttatatg
     4861 tagtcaaaaa cttagattcc ataagctgag ataaaagctg ttgtaatgaa aaataattca
     4921 tcatctcagt ctctaatact acttgagtta tttgagaatt agttaaatcc attttaattt
     4981 tttctaatat atataaaatt aaaagtttgt gataggctaa ttcttcagac gagttttcaa
     5041 acataaatat catcctccgt caaactattt ctatatataa tatattaaac taatttgtac
     5101 aaaattaaaa gaacaatttg tattgtatta ttaatcttta ataattcata taaaacaatt
     5161 ttataaataa atatagagaa acagggctgc taattagcag ccctgttgat acccaggtgt
     5221 caaaatttac tatttatttt ccagccattt gtctttcagc catttcaact aatttcttag
     5281 tcatatatcc accaacatat ccattttctc tagctgttaa gttaccttta tctatgtttt
     5341 catagttaga taaacctatt tcgttagcta tttctaattt catttggttt aaagcagctt
     5401 ttgcttctgg aactactgtt ctgtttgaat ttgacatagt aaatttcctc ctcaaataat
     5461 tagtttatga tgtctaacaa cacttttatt tatgtgatgt taatactaat ttctccaaaa
     5521 taaaaataat atatacagaa aaataaaggt aaaaatgtaa aaaatataaa aaaattaaat
     5581 aaatgagtat tttagagagt ttaattaggt caatataggt tttatttttt tagttattgt
     5641 cttataaatt aaatattaca gtactttttt gagctgaaaa aaaataaatt ttaaatatag
     5701 aagaaattat ttcaaagggg gaatagtatg aaaagagaag ttaaaaggac taaagctttg
     5761 atatatagtg ttttattttt ttcattatta ttagtgttaa tatttgtttt tgtttatgga
     5821 ataaaataca tatgttctaa atacgacaat tcaaaaatgc ctatatataa agttgataca
     5881 aatgagaaaa aaatagcctt gagttttgat gtagcatggg gaactgataa tatggatgat
     5941 attttaaaaa ttttagataa acataatgta aaagctacct tctttttagt tggtagttgg
     6001 gtggatgata atgaagaaat tgtaaaagct atagatgaaa aaggacatga aattggaaat
     6061 cattcaaaca ctcatgccaa tttgaaggaa atatcaaaag aagacataac aaaagagata
     6121 gaaactacat cagaaaaaat atataatata acaggaaaaa agactaattt atttagacct
     6181 ccttttgggg acgtaaacaa taaagctatg gacatttgta gtgatttagg ttataaagta
     6241 attaagtggg atgtagattc tatagattgg aaggagcttg gaccaaatca tgtaattgag
     6301 aaagtaataa aagaatctca acctggttca atagttttat tccatgctaa tataaatgat
     6361 gtagataact atttggatac tataataagt cgactaaaaa aagatggata cagtctagtc
     6421 aaaatatctg atttgttgta taaagataat tatgtagttg attccaatgg agtgcaaaaa
     6481 actaagaatt aataagtaat aaatgtatat tttaagtaaa gttgtgataa attatatagt
     6541 atatgttaga aaagtgtcga taaaaagacg gtgtctattt tttgacactt tttatacatg
     6601 tatgcaatat caataattat agattaaatt gattaaaaaa gtgtctaata attgactttt
     6661 ttttgatgga tttaaaaatt tagaagaatt aatgaatgct gtaagacagt atttccaaag
     6721 ggtataagtt tgtttaaaag ttggcataga aattgctttg ttaataagcg atatgttcta
     6781 tagaaataaa caatatgtta agaccttata atagaaaaat agcattaaca ttatttgaac
     6841 agcttataaa aaaattaata tacttaaggg ggcgtactag ttgaacaaac tatattctat
     6901 tttattaaaa caacacgttg gtggtccaga taagccagta gtaagtgttg gagatgtagt
     6961 aaaaaaagga actctaatcg cagaaccaac tggattaggg gcaaatattt atgccagtgt
     7021 tagtggaaaa ataagtgaaa taaatgacca agcgatagta attgaagctg atgaagtaca
     7081 agaagatact tttgaaccat taaaaggaga aggtatactt gacttaatta aagaagctgg
     7141 agtagtagga atgggtggag caggattccc tactcatgta aaattaaaca tagatttaaa
     7201 tggtggaaca atattagcaa atgcagctga atgtgaacct ttattagctc ataatataaa
     7261 agaaatagaa gaaagaccag aaatagttta tcaaggtata aaatatgcta tggaagttac
     7321 taatgctgga aaaggtatgc tagcaataaa aagcaagcat ccaaaagcaa tagaagcttt
     7381 caaaaaagta ataaagccag gagacaacat agaagttgca gaactagttg atatgtaccc
     7441 aatgggagaa gagagagcaa tagtaagaga cgttctagga aaattacttg agccaactca
     7501 attaccttca gaggctaatg cagtagtaat aaacgtagaa acactaacta gaatagtaga
     7561 ggctgttgag caaaaaagac cagttatatc taaaaatata actgtagttg gtcaattaaa
     7621 cagtggaaaa gagtctatag tatttgaaga tgtgccaatt ggaacaactg tcggagaatt
     7681 aattgaaaga gctggtggaa taaaaggtga atatggtgaa ataatcctag gaggaccttt
     7741 cactggtaaa gctactactt tagatgctcc aataactaaa actagtggtg gtataatagt
     7801 tactatgcca tttgttaatg aaaagagaaa aatgggtctt ttagtctgtg catgtggacc
     7861 taatgaagag agaatgagag atatagcaac taaaatggga gttactgata tagtatcagt
     7921 tcaaaaatgt aaacaagctc aagaaattaa aggtgcatta aaatgtgaaa atccaggaca
     7981 ctgtccagga caagctcaaa aatgtattga atttaagaaa gctggagcag aagttatatt
     8041 aataggaaac tgtactgact gtagtaacac agtaatgggt tctgcaccaa aattaaagtt
     8101 aggtgtatat catattactg accatgtaat gagaacagtt aatcacccat taataagaag
     8161 aataaaaggt gacgtaagat taaaaataaa ataatagata aatagaagtt agacatcttt
     8221 agaagtaaaa cttaaaaatt acttctaagg atgtttttta atgtattttg agggcaatat
     8281 atagctttaa aagaaatcaa aaaagtgcca aatgtaaaac taattaaacc atatatattt
     8341 aagaagggat tgaattggag ttgtgaatta tgttagtaga aaccattcta agtgtttgtt
     8401 attggtacat ttgatatttg tatgtaaata cagaaaaaac tttttgctca gatggatact
     8461 ttgctttcag tgtaggaaat gtttctaaag aaatgattga aaaatacata caaagtaagg
     8521 gataggttta catccaccaa cctaaaggta tagtggtttt acgccttaat ttataaaaag
     8581 aaattaattt atattattat taagtttaaa ataaaaagtc tcaaattgat ttataagtca
     8641 ttttgggact tttttatgta aatataatat gcaaatatag tttgtttaaa aatatctatt
     8701 ttaattatat tcttaaattt atgattggat acaggcattc attaattgtt tgatgaatag
     8761 aaaaatgttt tgaaggtgaa aatagtattg aaaaaataat atactaaggg tataattcat
     8821 ttgatttcat ttgtataaat ttaattataa attttaaagt gattatataa atttaaatgc
     8881 taattactta ataaatttta ataataatta tttaataaat aattattata atatgctaga
     8941 ataaatatat ataaataagt tttgatgagg tactactata aaggggggga ctttatgttt
     9001 tcgataccag aagtaaaaaa agtagaagag gtaatggata caaaatttac aactatagac
     9061 gaagacacaa gaattgaaga tgcaataaaa gaaatgataa aaagcaacac aaaaactcta
     9121 atggtaatag attcatctga ccaattaaaa ggcattatat ccatgacgga tatacataat
     9181 ctttatgaga tgcataaaaa gtatgaaggt caaccagtaa aacttataat gaagaaggat
     9241 gtaatatatg tcaacgaagg tttaacctta gatgaatgta gagatataat gatattaaaa
     9301 aatataggga ttcttcctgt tcttagagat aataaaatta ttggagttct aaaacaagag
     9361 cacattaggg actatctata tatgcattta gaagattatg gattgacttt gaagtatatt
     9421 ataggtcaaa ttaaagaagg aatttgtgct ataaataacg aaggtgttgt tatcttgtgg
     9481 aataagttta tggaggaaag gtatgatata aagtctgaag atatagttgg tcgaccgatg
     9541 aatgaatttt tggaaaatac tatttcagaa aaagtactaa atagtaaagt tggaatgagt
     9601 gacttgtatt ttacagataa aaaagaaaat atgtatgcat tagttcatgc aaatcctata
     9661 ttttataaag aagaatttat cggtgttgta tgtacagaag ttgatgttac agaagctaag
     9721 atattagctc ttgaacttga aaaagtaaat gatacattaa aatatctaaa gaacgaagtc
     9781 aaaaatttat ctaaaggtag ttttgataag attttaggta aaagttataa attggaaaaa
     9841 tcgaaagcga ttgcaaaaca agtggctaga acaaatagta gcatatttat ttggggtgaa
     9901 agtggtacgg gtaaagaagt atttgcaaga gctattcatg attatagtga gagaaaaggt
     9961 caatttatac ctgttaactg tagtgccata ccaaatgagt tatttgaaag tgagtttttt
    10021 gggtatgaat caggtgcatt tacaggagca agtaaaaagg gtcgaatagg tatatttgaa
    10081 cttgcaaaag atggaactgt atttttagat gaaattgccg atttacctct tagtatgcaa
    10141 gcaaagttgc ttagagtatt acaggaaaaa gaaataagaa gagttggtgg agataccaca
    10201 ataaaaataa atcctagaat aatatcagct accaataaag atttgagtaa aatggtaaag
    10261 gcagaaaaat ttagagaaga tttatactat agattaaatg ttgttgaaat aaagattcct
    10321 ccacttagag agagaaaaga agacatagga ttattggttc atagcttttt agaggagata
    10381 tgtaaacaaa ataataagcc tgttcttaca attagcaaag atgtaataga tatatttcag
    10441 aattatagat ggaaaggtaa tattagagaa ttaaaaaata cgattgaaaa tattgttgta
    10501 ttgtcacaaa attctaaaat agatgtagat gatgtaccaa gttatatgat ggattctaca
    10561 aataatagta ctgaagaaga agagtatccc ttagatttaa cgaaggcaac tcaaaaaatt
    10621 gaaataaaga atataaccaa ggcacttaaa atgtctaatg gaaataaagc taaagcagct
    10681 aagatattaa atatacccag aacaactttg tattataaga tagaccaata caaaatagat
    10741 gtgtctaaaa tatgacatat gactaaattt tgacaaaatg ataaacttta atttaaaacc
    10801 tagtaaagcc aacacttgta taagtgttgt gtatatagat gtgttaaaaa aaatgaaaaa
    10861 gtgttgatta attgacactt tttttgtttg agaaaatgtt tacaaataca aaaaataaca
    10921 tattttaaat aaatatttat ataatatttg gctaaaaata gcgatagatg aagaatctat
    10981 tatagaaaaa atctttttgt taataagttg gcataggaat tgcttataat ataaagtgac
    11041 tttgagtgag aaaaaaataa tattaatcat aacaaattat gtaggtttta atttacttaa
    11101 tttctcagtt aaggggttac ctgttgagga attgagattt aaaataataa ataagaaaat
    11161 aatgtaagga ggtattttat actatgtcaa taactttaga aacagctcaa gcccatgcaa
    11221 atgacccagc agtttgttgt tgtagatttg aagcgggaac aattatagcg ccagaaaact
    11281 tagaagatcc agcaatattt gcagacttag aggattctgg attattaaca ataccagaaa
    11341 atggattaac tataggtcaa gtactaggag ctaagttaaa agaaacttta gatgcacttt
    11401 ctccaatgac tacagataac gtagaaggat acaaagcagg agaggctaaa gaagaagtag
    11461 tagaagaaac agtagaagaa gcagctccag tatcagaagc agcagtagtt ccagtaagca
    11521 caggagttgc aggtgaaaca gttaaaatac acataggtga aggtaagaac ataagcttag
    11581 agataccttt atcagtagct ggtcaagcag gagttgctgc tccagtagca aacgttgctg
    11641 ctccagtggc aagtgcagca gcagaagtag ctccaaaagt tgaagaaaag aaacttttaa
    11701 gaagcttaac taaaaaacac tttaaaatag ataaagttga atttgctgat gaaactaaaa
    11761 tagaaggaac tactttatac atcagaaacg cagaagaaat atgtaaagaa gctaatgaaa
    11821 ctcaagagtt agttgtagat atgaagttag aaataataac tcctgataaa tatgaaactt
    11881 acagtgaagc tgtattagat atacaaccaa tcgctactaa agaagaaggc gaattaggtt
    11941 caggtataac tagagttata gatggagctg taatggtatt aactggtaca gatgaagatg
    12001 gagttcaaat aggtgaattt ggttcttcag aaggtgagtt aaatactact ataatgtggg
    12061 gtagaccagg tgctgctgac aaaggtgaaa tattcatcaa aggtcaagta acaataaaag
    12121 caggaactaa catggaaaga ccaggacctt tagctgctca ccgtgcattt gactatgtaa
    12181 ctcaagaaat aagagaagca ttaaagaaag ttgacaactc tttagtagtt gatgaagaag
    12241 taatagagca atacagaaga gaaggtaaaa agaaagttgt tgttataaaa gaaataatgg
    12301 gacaaggtgc aatgcatgat aacctaatat taccagttga gccagttggt acattaggag
    12361 ctcaaccaaa cgttgactta ggaaacatgc cagttgtatt atctccactt gaagtattag
    12421 atggtggtat ccatgcatta acttgtatag gacctgcatc aaaagaaatg tcaagacatt
    12481 actggagaga gccattagta ataagagcta tggaagacga agaaatagat ttagtaggtg
    12541 ttgtatttgt tggttctcca caagtaaatg ctgagaaatt ctatgtatct aagagattag
    12601 gtatgttagt tgaagctatg gaagttgatg gagctgtagt aactactgaa ggtttcggaa
    12661 acaaccatat agatttcgca tctcacatag agcaaatagg tatgagaggt ataccagtag
    12721 ttggtgtaag tttctcagct gttcaaggtg ctctagttgt tggtaataaa tacatgactc
    12781 acatggtaga caacaataag tctaagcaag gtatagagaa tgaaatatta tctaacaaca
    12841 ctttagctcc agaagatgct gttagaataa tggctatgct taaaaatgct atagaaggtg
    12901 tagaagttaa agctcctgaa agaaaatgga atccaaatgt taaattaaat aacatagaag
    12961 ctatagaaaa agttacagga gaaaaaatag tattagaaga gaatgagcaa tctctaccaa
    13021 tgagtaagaa gagaagagaa atatacgaaa aagacgaaaa ctaaaattta gatactaact
    13081 aagatttaga caataacata aatttagatc caagtttaaa acagtataga gaggtagaat
    13141 tataatggat aggttaaaat atatctcatc agagactttt tatgaaggta ttatagttga
    13201 catcaaaggt ggtggagtga ctatagacct aaaaggtaga cttggacaat ttaaaatacc
    13261 taatagaatg cttataactg actacgaact taagataggt caagaagttg gattcatgct
    13321 aagctatcct gaggtattaa gtccagagcc taatcaagag tatgtagaaa atattaggag
    13381 agaaaaagaa aaacaagcag aaatgcaatc aaaacaagaa gaaaaataat aaataatcgt
    13441 ttgaaaatag aagggagagg aaatatatga gccttacaac agtacaagga cttcaatctg
    13501 aaatattcgt tccaataaca cctcctccag tttggactcc tgtaactaaa gaattaaaag
    13561 atatgactat agctttagct acagcagctg gtgttcattt aaaatctgac aagagattta
    13621 atctagcagg agatactaca tttagagcta tacctaatac agctacagtt gatgaaatga
    13681 tggtatcaca tggtggatat gacaatggag atgtaaacaa agatataaac tgtatgttcc
    13741 ctatagatag attacatgaa ttagctgcag aaggatttat caaaggagta gctcctatgc
    13801 actatgcatt catgggtggt ggaggaaacc aacatgtctt cactgaagaa actggtcctg
    13861 ctatcgctgc taaacttaag gaagagggag tagacggtgt agttatgaca gctggctgag
    13921 gtacttgcca tagaactgcc gtgatcgtgc agagagcaat agaggaagct ggaataccta
    13981 caataataat agcagctctt cctccagtag ttagacaaaa cggaactcct agagcagttg
    14041 ctccattagt tccaatgggt gctaatgctg gtgaaccaca taatatagaa atgcaaacac
    14101 atatattaag agatacatta gagcaattag ttgcaatacc atctgctggt aagatagttc
    14161 cattaccata cgaatataaa gctcacgttt aaaatgtaat ttatatttga aaatatagaa
    14221 cccttctcct acacggttag gagaggggtt ttaagaacaa aggtggatat tatggaagaa
    14281 aaaataatga gaagacttgt tataaaaccg tttcatatga atgaagttaa tttcggttca
    14341 aaaacttcta taaagaaaga tgtacttaca atagatttat caagtataga tgaaataaaa
    14401 gaaagagaag agttaataac agacataaaa gtagacatta taaaacctgg agattatgat
    14461 agagaaatta atactattat ggacattata ccaatatcaa caaaagtttt aggcagactg
    14521 ggagagggta tcacacatac tttaacgggt gtatatgtaa tgttaactgg tgcagatgaa
    14581 gatggaaatc aaatgcatga atttggttct tcagaaggaa ttttgaaaga ccaaatggta
    14641 tttgggagat atggaactcc atcagttgaa gattatataa tccatgtaga tgtaacttta
    14701 aagggaggtt taccttttga aagaacactt ccacttgcag cattcagagc atgtgatgat
    14761 tttatacaag aaataagagc gtctttgaaa atggaagacg gaagaaatgc tactcaagtt
    14821 cgtgaatatt ttgacaaaat tcgaccaaat gcaaaaaagg ttgtcatagt aaaacaaata
    14881 gcaggtcaag gtgctatgta tgacaatcag ttattctcaa aggaacctag tggatttgaa
    14941 ggaggaacat caattataga tatgggaaat gtacctatga ttatatctcc taatgaatac
    15001 agagatggtg cccttagagc tatgacttaa gagaggtgga tattatggga ataggacctt
    15061 caacaaaaga aacatctctt catcacttta gagacccact tttagacata gttgaaagtg
    15121 ataaggatgt agatttactt ggagtaatag tagtaggaac tccagatgga aatgaaaata
    15181 aaacctttgt gggacaaaga acggccgctt ggctggaagc catgagagta gatggagcta
    15241 tagtttcatc agatggttgg ggaaactcac atgttgacta cgctaacaca tttgaggaaa
    15301 taggaaaaag agatatacct gtagttggag ttacatttaa tgggactcaa gctaagtttg
    15361 ttgtaagcaa tcaatatatg gataccatag ttgatatgaa caaatcaaag gaaggtattg
    15421 agacagaagt agttggagaa aacaacacaa atgaaataga tgctaaaaaa gctttagcat
    15481 ttttaaaact taaaatgaga aaacatggat aaaaaattta agtaagggaa tgagttttgt
    15541 gggaatttct acatcaatga aaaacacatc acttcatata tttagagacc cgttactaga
    15601 tttgatagat aatgattctg acatagactt gttaggagtg gttgtagttg ggacatcaga
    15661 gcacaatgaa tggaaaacat ttttagcaac tagacttgga agatggattg aatcattgcg
    15721 acctgatgga gtgattataa ctttagatat ggcaggaaac cagcacattg attttacaaa
    15781 tgctatagca gagtttgtaa aatcagatat acctgcagta ggtcttacta ttatgggagc
    15841 agatggatta gtaattacca atccatattt ggataaagct accataattg attataaaaa
    15901 gacaacaggc tacatagaaa cagaagtagt tggagacaat cacatggatg aagttgatgt
    15961 taaaaaagca ttggctttcc taaaattaaa aatgcgtaaa gacgcaaaat aaacctaagg
    16021 gggaatttga tatgaaattt agcagaagta tacaagctat agactctcat acagcaggtg
    16081 aagcaactag aatagttgta ggtggaatac ctaacataaa aggaaattct atgcctgaga
    16141 aaaaagaata tttagaggaa aatctagatt atttaagaac tgctataatg ttagagccaa
    16201 gaggacataa tgatatgttt ggttctgtaa tgactcaacc ttgttgccca gatgctgact
    16261 ttggaataat cttcatggat ggtggtggat accttaacat gtgtggccat ggtacaatag
    16321 gagctatgac agcagctata gaaacaggtg tagttccagc agtagagcct gtaactcatg
    16381 tagttatgga agctccagca ggaataataa gaggagacgt tacagttgta gatggaaaag
    16441 ctaaagaagt atcattctta aacgtaccag ctttcttata taaagaaggt gttgaagttg
    16501 acttaccagg tgttgggact gttaaatttg acatatcatt tggtggaagc ttctttgcta
    16561 taatacatgc aagtcaatta ggtttaaaaa tcgaacctca aaatgctggt aaattaactg
    16621 aactagctat gaaacttaga gatataataa atgagaaaat agaaatacaa catccaactt
    16681 tagctcatat aaaaactgta gacttagttg aaatatatga tgagccaact catccagaag
    16741 ctacttacaa aaatgtagtt atatttggtc aaggtcaagt tgacagatct ccatgtggaa
    16801 ctggaacaag tgctaaatta gctactttac atgctaaagg tgaattaaaa gtaggagaaa
    16861 aattcgtata tgaaagtata ttaggaactt tattcaaagg tgaaatagtt gaagaaacta
    16921 aagttgcaga tttcaatgct gtagtaccta aaataactgg ttctgcttat ataactggat
    16981 ttaatcattt tgtaatagat gaagaagacc cacttaaaca tggatttatt cttaaataag
    17041 taataaataa attaggaaat taaaatagaa tagtagccac ttgtcggttg ctattctata
    17101 gcttttaggc tataagattc agaatatatg aaaaaataaa aaaataggag gatggattat
    17161 ggtagcaatc ttaaaggcat tatatgtagt attcgcagca atctttgcag ttgtgtttgg
    17221 taaagacgta gctaaagcaa acaaagaggg gaaactagaa gaacaaagta ctccaaaagt
    17281 tgctgtaaca ggatttataa cagacttctt tgatacatta ggaatagggt catttgctcc
    17341 aacaatagca atgtcaaagg ctttaaagtt aaatatacca gataaaaaaa tgcctggtac
    17401 attaaacgta gcacatacta ttccagttgt tacagaagca ttcatattta catcaatcat
    17461 accagttgat ggtgttactt tagtatctat ggttgtggca gcagcagttg gttcatatat
    17521 aggtgcagga ataatagcta agatggacga gagaaaaata caacttgtta tgggtgtaac
    17581 tttagcatta acagcaatat taatgttatt aggtcatcct tggattaacg tattaccagg
    17641 tggaggaaat gaattaggtc ttactggtgt taaattggta ataggtgtaa taggtaactt
    17701 tatactagga gctttaatga cagctggtat aggtctatat gctccaggta tggcaatggt
    17761 ttatttctta ggaatgtctg caaaagttgc attcccaata gttatgggtt cttgtgcatt
    17821 attaatgcca gttgcaagta tgaaatttat aaaagaagat gcatatacta aaaaagcatc
    17881 agttataata gctatatgtg gtttaatagg agttttcgta gctgcatata tagtaaaaac
    17941 tctaccaatg gatatattaa aagcattagt aatagtagtt atagcttata cttctgctac
    18001 aatgataatg gctgcaaata aaaacaaaaa gactgcttag ttaaagacta cttagtgttt
    18061 attaaatatt acagctaata ataaagatta taaactgctt taaaatattg aattaatatt
    18121 ttagggcagt tttttatttg ctaaattaat ataaagcaat taacatgcca attatgtaat
    18181 atatatagtg atttagtaga tttaatgtaa aaaacgttgt attttcaaag aataaagcat
    18241 aatttaaagc taaatattta aattttataa tcaataagtt caataaatgt tcatatatta
    18301 atacagatta ataaaaatat aagctgtaat aagtctaata taacttataa ttaagaaaaa
    18361 taagtttata atgactcaat attcatctga attatttgaa taaatttaat tcgttgaagt
    18421 tgcttatata gagtgaataa atttaagtta tatatgactt attaattata taataggatt
    18481 tttataatta atacaaaact gaaataggta gtttaattag tatatgtagt acaaaaagat
    18541 aataatttta taatta
//
LOCUS       DAELSY010000075        18317 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.075, whole genome shotgun sequence.
ACCESSION   DAELSY010000075 DAELSY010000000
VERSION     DAELSY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18317
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.075"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(264..1361)
                     /locus_tag="KPG09_002261"
     CDS             complement(264..1361)
                     /locus_tag="KPG09_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG0966869.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILFTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            1654..3180
                     /gene="glpK"
                     /locus_tag="KPG09_002262"
     CDS             1654..3180
                     /gene="glpK"
                     /locus_tag="KPG09_002262"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG0966870.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFESGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKEIKMLRVDGGITKNNFTMQFQSDILDIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTKQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            3221..4336
                     /locus_tag="KPG09_002263"
     CDS             3221..4336
                     /locus_tag="KPG09_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBG0966871.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(4617..5381)
                     /locus_tag="KPG09_002264"
     CDS             complement(4617..5381)
                     /locus_tag="KPG09_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG0966872.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            5640..5730
                     /locus_tag="KPG09_002265"
     tRNA            5640..5730
                     /locus_tag="KPG09_002265"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5674..5676,aa:Ser,seq:gct)
     gene            5818..6606
                     /locus_tag="KPG09_002266"
     CDS             5818..6606
                     /locus_tag="KPG09_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBG0966873.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHIGVLFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSVDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEISE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            6627..7367
                     /locus_tag="KPG09_002267"
     CDS             6627..7367
                     /locus_tag="KPG09_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG0966874.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDSLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAVVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            7545..7919
                     /locus_tag="KPG09_002268"
     CDS             7545..7919
                     /locus_tag="KPG09_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966875.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKDNKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            8011..9471
                     /locus_tag="KPG09_002269"
     CDS             8011..9471
                     /locus_tag="KPG09_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBG0966876.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTIGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIEEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLNVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFETWFESFKAELENAIKK"
     gene            9524..12400
                     /locus_tag="KPG09_002270"
     CDS             9524..12400
                     /locus_tag="KPG09_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0966877.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKILNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILNDKNINDKPIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQEGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRIEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKDKKLKWIRMNIFEDELKVLG
                     VVVDVTKEFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLD
                     NLKYINDTYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRD
                     IINSVKKQMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTI
                     KGEVSEFDINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPK
                     IKTFSSPIDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLS
                     KVDFEEIEDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNG
                     DAVLLYIMPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVISEGVETKGEME
                     TLINLGVDYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            12477..14543
                     /locus_tag="KPG09_002271"
     CDS             12477..14543
                     /locus_tag="KPG09_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG0966878.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVYTANRLIDKNKNRYEKVIKCSQGDGGSVTYISPHDSEEEAL
                     YIAKEIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     misc_binding    15135..15345
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15571..16386
                     /locus_tag="KPG09_002272"
     CDS             15571..16386
                     /locus_tag="KPG09_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG0966879.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            16414..17091
                     /locus_tag="KPG09_002273"
     CDS             16414..17091
                     /locus_tag="KPG09_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG0966880.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            17150..17872
                     /locus_tag="KPG09_002274"
     CDS             17150..17872
                     /locus_tag="KPG09_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG0966881.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
BASE COUNT         7214 a         1932 c         3247 g         5924 t
ORIGIN      
        1 caagaatata aaatgactga ataaaaaacg attgcgtcaa aaataaaaaa caattaaatg
       61 taagaataat tatgtcaaaa atataaaata aattaaaaga aaaagattat actgaaggaa
      121 aaagatttga aataggggtg aactttacat attatagttc acccttatac aaaaatataa
      181 aatataattt attataggaa aatttaaaaa aatcagtcta tttaatttat actgatgtat
      241 tgtaggattt tacaaacaaa ctgttatata acttttttta tctccaattt tgattttaag
      301 atatctaaga atttataaca atcttctgga tatatttctc ccatatttgc aacttggaat
      361 atgttagcat ctaggaattt gccttttcct ggatagacaa catagccact ttccctttct
      421 aggcctaata taaactcatt tacattgatt tctttaggta gaaatacaga tgttactgta
      481 ttagacatat acttatctgg aagtaaaaat gttagcccca attcttccat gccattcctt
      541 aaaatagcag aacattcttt gtatctttct attttactgt catcttccaa aaactcagtt
      601 aaagcttcat taagggcaaa tattaaagta acattaggtg tatttggtgt ttgagaagat
      661 gatttagcaa ctttatagtg tttataaaga ttaagatata cattcttaca ttgattttct
      721 ttaatatttt gtaaaatact ctctttagca caaaggtatg cagaacctgg aaatgctcca
      781 agagctttac caccaacacc tgttattata tctatattaa actcttctac atcaatatat
      841 tcacctgctg aggcacttat tgcatctaca aaaaatattt tgttatactt cttagcaagt
      901 tttcctagtt cagggactgg atttatcatt cctgaactag tttcatggaa caccatagca
      961 attactttta tatcagaatt ttcaattata tatttttcta aaatgtccag gtctgggtat
     1021 tctgcccatt caaaattagg cttatatgtt tttacattat aagtaaataa tatttcttct
     1081 agcctttctc caaaaacacc attactaact agcagaactg catcttcttt gtcaaagata
     1141 gaagatagta cacattcatt tacagatgtt ccagagccag atacaattaa tgagtagtaa
     1201 ttagaagttg cattatatag tttattgatt ttttgttgtg tatctttgaa taaatccata
     1261 aactcgtttc ctctatggca tatatcatag tgaagaagtg aatttctgac tttatctgta
     1321 accattactg gtccaggact gaatagatat gtttttttca ttgtaaagcc tccttaaaaa
     1381 gttaaattta aattttcgaa tgactgacta tgtgtaaata ttaaaatatt gtgtgttatt
     1441 ttaactagat tatagaataa aataatgaat attgcaataa tatttcaata aaaatacttt
     1501 tttaatcacc ataattaaaa ataacaaaaa atattgtaat gaatattgca taaatccaca
     1561 tgatgatata aaatataaat aacttgaata gattatatat tcaaaaaata aaaaagaatt
     1621 tattaattct gaaaataaag ggggacattt taagtgaaat atgtattagc aattgaccaa
     1681 ggaacaacag gaacaagagc agtattgata aacgaaaaat cagagtttgt ttttagcgat
     1741 tatatggaac atactcaaat atatccacaa tctggatatg ttgaacatga ccctatagaa
     1801 atttggaata atacaaaaac tgtgagtaat aatgtattac aacaggcttt tgaaagtgga
     1861 atcaatgaaa gtgatataaa aggaattggt atagataatc aaggtgaaac agtaatgtta
     1921 tgggataaaa atacaggtaa accactttac aatgctatcg tatggcagtg taggagaact
     1981 tatgattatg tggaaaattt aaagaatata ccaggtttag aaaataagat aatagataag
     2041 actgggttaa ttatagaccc atatttctca ggtacaaaaa ttaaatgggt aatagataat
     2101 gtaaaaggtg taaaagaaaa aattaaaaat ggagaagtac ttgcaggaac attggatagt
     2161 tggttaatat ggaaaatgac aggaggaaaa tcttttttaa ctgatgtatc tacagcagca
     2221 agaacaatgc tttttaatat aagagatatg gattgggata gagagttgct tgacattata
     2281 ggagtgccta gaagtatttt agcagatata atgcctacta gtggtgattt tggaactaca
     2341 gataaggagg cattttgtgg ggtttctata aaaattactg cttcaatggt agaccaacca
     2401 gcagctttat ttggcaatgg atgttttgaa ccaggaatgg ttaaaaatac ttatggtaca
     2461 ggatgtttct tatatatgaa tataggtgag gagttaaata tagcaggtaa tggtattttg
     2521 acaacagttg cttggaagat aggagataaa gttacatatg cttatgatgg tggagtgtat
     2581 atagcaggtg ctgcaataca atggcttaga gatggaattg gagttataaa aaattattca
     2641 gaaactgatg acatggcaaa ttccatatca agtactggag gggtatattt tgtaccagca
     2701 tttgcaggta tagcagcacc ttattgggac caatatgcta gaggcacaat ggttgggata
     2761 acaggaagta ctaaaaaaga acatatagta agggctactc ttgagtctgt tgcacttcaa
     2821 gtcaaggatg ttgttgattc catgaatttg gcttcaggga aagaaataaa aatgttgaga
     2881 gtagatggtg gaattactaa aaataacttt acaatgcaat ttcaatctga catattagat
     2941 ataccaattg aaattgcaga aaatcctgaa acgacagtac ttggtgtagg gtatatggca
     3001 gggctttcct gtggtgtttg gaacagtatt gatgaaatta gaagtgaatt tacaataagc
     3061 aaaaaatata ttccaaatat gactaagcaa aaaagagaag aaatattact tggatggaat
     3121 caagcagtaa aaagagctat gaattgggaa aacgatacca aaaaggtgtg tgctaattaa
     3181 gttacaatag agtattttac atgtatagga ggaattaaaa atgccctttg ataaaattat
     3241 attatacata atggctgtgg gagtgctttt aggagcgtta gatagaataa ttggaaataa
     3301 atttggactt ggagagcagt ttgaagaagg gtttaattca atgggtccat tggctttagg
     3361 aatggttggt atagtttgtt tagctccagt aatttcagat gtactaggtc ctataataat
     3421 accaatattc aatctctgtg gagctgaccc atctatgttt gcaactattt tagcgaatga
     3481 tatgggaggg tatccattgg ctatggaact tgcacacaat aaagaggcag gaatgcttgc
     3541 aggattagtc gtgtcatcca tgcttggatg tacattagta ttttcaattc cagtaggact
     3601 aggtcttata gaatatgaag acagaccatt ctttgcaaaa gggttactga taggcttgat
     3661 aacaatacct tttggaggta ttataggagg attaatagca ggatttaact tatcaatggt
     3721 aattataaat atgataccag tgataatatt ggcagtaata ttggcagtag gtttaaaatt
     3781 taattctaca cttatgataa atgggtcatt agtttttgga aaatttatat ctatattgat
     3841 aacgataggt ttgggagcag cagcatttca agaaataaca ggtatagtac ttataaaagg
     3901 catgacacct ataagagaag gtattcaagt aattggaagt attgcaatag tgttattagg
     3961 tacttttcca atattacatt tattagttaa agttcttaat aaaccactta caatagtggg
     4021 tggaaaatta ggtatggatg ccactagtgc agcaggatta gtttttactt tagccaatag
     4081 tattccagta tataaaatga tgaaagatat gagtccaaag gggaaagtag taaatactgc
     4141 ttggctggtt tgtgctacag cagctttggg ggaccattta ggatttacag caggagtagt
     4201 tccagagatg attacacctg ttgtaattgg aaaattggtt ggaggagtat tggcaattgc
     4261 acttgctata cttatgacaa aagatttaac tttggaagaa gaacaaagta ttaaaatgaa
     4321 gaaagatgca gtgtagaata aacaatactt aagtcaaatt taagttattt taaagtagga
     4381 gtttaaataa ctaaaaataa attaaaaaga cagacaatta aaaaataatc gaataaccaa
     4441 aactgataaa ttaaataaaa tggcaatcaa ataactaaaa atgataagct aactgaaagt
     4501 agttacataa ttaaaatata aagtatagtt tagacataag tggagctaaa taaataatta
     4561 tttggctcct taattttaat ttattacatc aaggaactaa aaatcaatca tacaagctaa
     4621 ttatcagcta tgactaaagg tattcctttt tgagaaaact ctattattat attatggtct
     4681 atactagcag aggtaataat agtatctatg tgttctatag gagctactga tgcaaatgaa
     4741 tttataccaa acttaggagg ttcgacaact actatagatt ttctagacct ttcaatcatt
     4801 ttttttctaa ccagtgcttc ttctaagtga tagtcagaga ttccattttc aagagtgata
     4861 cctccagcac ctaaaatagc tttatcaaca ttaaattgtt caatagctga gagtgtaagt
     4921 ggaccagaaa aagaaccttc agtttctcta agatagccac caagcacata tacttttata
     4981 ttagtattca taagttcatt tgcaactaaa attgagttgg ttattacagt tagattcttt
     5041 ttatctttca aattttttga aacttcaagt acagtagttc cagtatccat aaaaatagta
     5101 tcatttgtat ttataaactc actacattta atagcaatct gttttttatc gtttgagttt
     5161 attttttttc ttttaacata gttaagttct acgctacttg ctataactgg tatagcacca
     5221 ccataaactc tttttaaaag accgatactt tccatatggg ctaaatctct tcgtgcagtt
     5281 tctattgata tatcaaacat ttgaactatg tcatttattt taattatatt atctgatttg
     5341 agaatactta aaatcttctc gtatctttcc tgtggtaaca taaaatatcc tccttggact
     5401 agtattattt tagttttggc ataacttaca attaagtcag agtttaggta tatttgttaa
     5461 tgtatgtata tttatcaaat aatattataa gtgttatgca agataatttt accatattta
     5521 aaacttacat ttaagcaaaa aagtaaaaat agatgtttaa taattcaaaa taactcaaat
     5581 tttataaaac aattgactaa aatatgattt tattatataa tttactagta tagagatatg
     5641 gagaagtact caagtggctc aagaggatcc cctgctaagg gattaggctg ggtaaccggt
     5701 gcgagggttc gaatccctcc ttctccgcca attgttaaag gctattttta ttaaagatag
     5761 tctttttata atgtcataaa tgattagata tgtataatta aagaaaggag ggaatagatg
     5821 acaactttaa tttttttcat agtggggata gcatctttgg tagttgcttt tgttattgat
     5881 gggggacata ttggagtatt atttgtattt tcatcagcac ttgttgtgtt tggtggaact
     5941 ataggcgctg ttggagcttc gactccattc cctgtgttta aaaagtctat aaagataatg
     6001 ttattagctt ttaaaaataa aaagcctaat aacatagaaa atataatcta tttcaaagaa
     6061 attgcaacaa aggctaggaa agaggggctt cttagtgttg acaaagaaat aagtgagaat
     6121 gatgaattag acccttttat tagaaaaggg cttgaactta tggtagatgg ggtggatgca
     6181 gttactataa gaaatatatt ggaatcacag atacagataa catcaaaacg acataaagaa
     6241 ggaatagcaa tttttgattc agcaggtgga tatgcaccta cactggggat aataggtaca
     6301 gttatgggat tagtacatat tttaggtaat ctttcaagta atccagatgc attaggacct
     6361 caaatttctg ttgcattttt ggctactttg tatggtgttg gttctgccaa tttaatatgg
     6421 ttaccaatag catcaaaact taaggctata aatcaacagg aaataagtga aagacttttg
     6481 gtgatagaag caatattatc catacttgag ggtgataatt ctaatttaat gacagaaaaa
     6541 ttaaaaatat ttttaaattc aaaagaacta attgaacttg aagaaattgg tggtagagct
     6601 gaataatata aaaggatgga atgtttatgt tgtatgatga agatgaaaaa gaagaaaaca
     6661 atgagcgatg gcttttaact tattcagacc tcataacatt acttatgatt ttctttgtaa
     6721 taatgtattc tatgagtaat gttgatgcgg agaaatataa acaattatct caatctctaa
     6781 attctgcttt tggtggttct tctggagtta ttgaaggtgg taatagcaaa atagaaccag
     6841 tagttgaacc aggaagtaat gatttagata gtttacaaaa tgctaagttt aaaaaagttg
     6901 gtgaagaaat ccagaagtat ttaaatgaaa atggtatggc taattcagta tcattaagag
     6961 ttcaagatag aggattagta ataagcctta aagatacaat actatttgat acaggaaaag
     7021 cagttgtaaa ggataattct agagataaga ttattcaaat aggtaagatg ttaaatgaaa
     7081 tgaatagtta catgagagta gaaggtcata cagacaatat gtctataaaa aattcagagt
     7141 ttaagtcaaa ttgggattta tcagttatga gagcaaccaa tgttgttcaa cttcttattg
     7201 acaatgcagg tatagcacca gataaattat ctgctgtagg atatggtgag tttagaccaa
     7261 tagcagaaaa ttcatctgaa gaaggtaggt caaaaaatag aagagttgat atagttttag
     7321 ttgatagtaa atatgataat gtagaaaatg tttcaaagga taaatagtta aattagctgt
     7381 ctcaatacag aattaattct aaaggagatg gcttttttat gcgaaaatta aaaatagata
     7441 catagtaagt tacctttttg taggaatata tggtatctta aatatataat atttaaataa
     7501 tatataagct caattaaaaa aattttatag aggatgggaa ataaatgggg aataaaaata
     7561 aaaatacgaa atataaagat aaaacaagag aaagatataa taaaaaagtt gagaatttaa
     7621 ataaagaaaa attaaataaa gataataaac agtctaagaa ggtagataag attgatgata
     7681 ttattaataa atctagtatt gtattatgtg tacttctagt ggtgttttca ttactttact
     7741 tatttaaatt ggttcaactt aatataataa tgatactttt agtaattaca gtagctattc
     7801 aacaaggatt ggtgtcattt ggtgcattta gaaataaaaa ttatgtaaaa gctttatttt
     7861 ctatatttgt aatagtatgt ttcttaattg cttttaaata tgttgtaaat cctgtttaaa
     7921 tatatcaata aacgttctat aattaatata aatagtgaag ttacagtttt aatgctatta
     7981 taaattttat aatttgaagg agtagatttt atgaataggt gtaagtttgc aaaattaatt
     8041 atcacatcaa tgattgtttt attacttaca ggatgtagtt taaaaaagga agataattat
     8101 aagttagacc cagaaaatcc gacatcaata caaatttggc attattataa tggacagcaa
     8161 aaaatggctt ttgataagct tgttaaggaa tttaatgaca ctataggtgc tgaaaaagga
     8221 attattgttg aagcgtttag tcaaggtaat gtagttgatt tatcagagaa agttttagat
     8281 tctgcaaata aaaaagttgg ctctgaagat gtgccaaata tatttgcagc atactcagat
     8341 actgcatatg aaatggataa attagggtta ctagtagata tggataagta cataagtaaa
     8401 gaagaattaa atgaatacat agacgaatat gtagaagaag gcagaataaa ttctaaggat
     8461 gagtttaaac ttttcccaat tgcaaaatca actgaaattt tgatgttaaa taaaactgat
     8521 tgggataaat ttgcaaaagc tacaaattca aatataaatg atataaaaac aattgaagga
     8581 ttagtagaga catcaaaaaa atattatgaa tggacagata gtttaacgaa agagcctaat
     8641 gatggtaaag cattttttgg gagagatgct ttagcaaatt atattattat aggaagtaaa
     8701 caactaggag tagaaatttt ttctgttaaa gatggtaaag ttacactaaa tattgaagaa
     8761 aaagttatga aaaagctatg ggataatttc tatgtaccat atataaatgg atattttgct
     8821 tcttatggta agtttcgttc tgatgatgca aaaacaggtg atatattagc tcttgtaggc
     8881 tctagttcag gagcagcata ctttccagat agagtaatgc taaatgataa tgagagttat
     8941 aatattgaaa catctgtttt tcaagcacca cgttttaaag gagaaggaaa atatgctgta
     9001 caacaaggtg caggaatggc tgtaacaaaa tctactaaag aagaagaata tgcatcagta
     9061 gaatttttaa aatggtttac agataaagag cctaatataa aattctctat tgcttctgga
     9121 tatttaccag taaagaaaaa aacgaatgat ttgaatgtta taaaagaagt tatgaagaaa
     9181 gataatttga gtttatctga taaagttgaa ggttcaatag cctcatctat tgaacaaatt
     9241 aacagttgta atttatacac taataaagca tttgatggtg gaactaaagc tagacaaata
     9301 cttgaatatt caatgcttga taaagctaaa gaagattcaa ataaaataaa tgaattggta
     9361 aaaagtggaa cttctaagaa ggatgcggta gcacaattta ataatgataa gaattttgaa
     9421 acttggtttg aaagttttaa ggctgagctt gaaaatgcta taaaaaaata gatgaattgg
     9481 cagttagtgt gtagacaagc acaaaggtat aggtggaata agtatggaaa acaatctaag
     9541 aaaaaacaat tcaataatga aaaagatgat atttccccta attattgtta tgctaattca
     9601 aacaagttta ttttgtgcca caattttatg gggaggaact attaagaaat taaatgataa
     9661 ttcgtttgac atcttaaatg aaagagtaat taatcgtaaa aattatatac aaaatgaaat
     9721 gttacaaaga tggtcaaata caagtgaaac ggaagctaca attaattcct ctgtaaaaaa
     9781 aatattaaat gaaaaaaatg ccttaattga ggatgtaggc aacaatgaag aaattaacaa
     9841 agatattata aaaagcatat caaatgattt aatatatctt ttgagaaaaa actctgttac
     9901 aggagctttt ataattctaa atgacaaaaa tattaacgat aagccaatag atagtaaagg
     9961 tgaaataagt aggactggat tgtatataag ggatttagac cctaagttta atccaaatga
    10021 taattctgat ttgttaattg agagaggttc atcagatata gcaagaagtt ttggaatatc
    10081 tatggacagt tattgggaac ctaaattttc atttaaaagt gaaaatgagc aagagggaga
    10141 tgattttttt tataaaccat ttagagctgc aattgataat ccaaacgtat caagttctaa
    10201 tttgggatac tggggaagta agtttttatt gacaaatgca ggtgataggc ttatgacata
    10261 tacagttccc cttatttatg aagatggaac tgtatatgga gtacttggaa tagatttaag
    10321 tgtaaattat ttaagagaat tacttccata ctcagagata aatggaaata aaaaaggtaa
    10381 ttatatttta ggtgtagata gaaataatga tatggccttt gaaaatgtaa tttcaaatgg
    10441 atactcatta aaagaaaaat tttgggatgg tttacattct aatttagagc agaaggaatc
    10501 gtataaaaat atatataaga ttaaaaataa agataatgaa tgtggtaatg gcaacattta
    10561 tgctagtgta caatacttag atttatataa ctccaatact ccatttgaaa atgaacgttg
    10621 ggctcttttg ggtattattg aagaaaaaag tcttttgtca ttttcaaaaa ggatagagaa
    10681 attagttata atatctatag caatttcttt attaataggt ctaataggta tttacattgc
    10741 atcaagatta tttacaaaac caattgttct tttagccaac aaagttagaa acagtaatcc
    10801 atcaaaacca gtaaatcttg ataagataaa tatatctgaa atagatgaat tatcttcagc
    10861 tatagagtta ctcagtgcaa atgttgcaga ttcttcatca aaattatcac agattattga
    10921 aatggtagat atgccaatcg gggcttttga gttatacaaa aacgcagaga atgttttttg
    10981 tacaaaagat aaaaaactta agtggataag aatgaatatc tttgaagatg agctaaaggt
    11041 tcttggtgtt gttgttgatg tcacaaaaga atttattgag aaaaggaaga tagaatatga
    11101 aagagatttt gatattttaa cgaatctttt aaataggaga gcttttcatt cacagataca
    11161 agaaaaattt aagaataaag atgaacttaa gatagcagca tttattatgt gggatttaga
    11221 taatctaaaa tatattaatg atacatatgg acatgatgct ggtgatgaat atattaggtg
    11281 tgctgcagac atacttaaga agtttatatc atggaatggt atagtatcta gactttcagg
    11341 tgatgagttt tatgtattta tatatggata tgaaagtaaa gatagtataa gagatattat
    11401 taattctgtt aagaaacaga tgaataatac cttattaaaa cttcatgatg gaacagaatt
    11461 taaaataagg gcatcagcag gaatttcctg gtaccctgat gattcaacca actatgatga
    11521 attaatgaga tacgcagatt ttgctatgta tcaaattaaa aatacaatca agggggaagt
    11581 tagtgaattt gatattaata tatataataa ggattcattc ttattgagta atagagaaga
    11641 attaaacaag ttaattgatg gtcaattagt tgagtatgca tttcagccta ttgtagattg
    11701 taaaggtaaa gtatttgcat atgaggcact tatgagacct aaaataaaaa cttttagttc
    11761 accaatagat gtaattagat tagctcgttc acagtctaaa ttatatcaaa ttgagcgtat
    11821 tacatggttt aaagcactta aatcatttaa aaaatacaaa gaaaaatttg gcgattgcaa
    11881 actgttctta aattctattc ctaataataa tttatcaaaa gtagattttg aggaaattga
    11941 agatttatat ggtgaatatt taaatagagt agtattagaa ataacagaga atgataagat
    12001 taatgaagat tttattgaga aaaagaaagc atatatagac cattggaata tagaaattgc
    12061 tttagatgac tttgggacag gttacaacgg agatgctgta ctattatata taatgccaga
    12121 ctatgtaaag attgatatgt ctattgtaag aggtattgat aaagatgaaa acagacagaa
    12181 aatattaaaa aatcttatct catattctaa cgaaagaaat attaaagtga tatcagaagg
    12241 tgtagaaaca aaaggtgaaa tggaaacact gattaatctt ggagtagatt atatgcaggg
    12301 ttactatatt agtaaaccat catttatacc tgaagatata agtgacaaaa ttaaaaaaga
    12361 gctcaagata tgtcatgaaa ataggaatga tgtggtttaa tagaggaaaa attaaattta
    12421 tttttatgaa cttttagttt ataattaaaa agaaaataat ataattataa attaaggtgg
    12481 tggagctaat aatcacaaca aacatagata taagaaagtt aaatgaaaat caattagaag
    12541 cagttgaaca tatagatggg ccttgcatga tacttgcagg gcctggttca ggtaagacca
    12601 gagtaattac atatagaata gccaatatgg ttgtaaacaa aaatatagca ccaacaagaa
    12661 tcttggcaat aagttttacc aaggcatctt caatagaaat gaaaaataga gctttgaatt
    12721 taagtgatga tataagactt aataaggtaa cctatggtac ttttcactct gtatttttta
    12781 aaattctaag atactttgaa agatacaatt tagacagtat atttgatgaa aagtcaaagc
    12841 gaatgacaat caaggcaatc ctaaaaagtt taaatataga gaatgctgat gatgatgaga
    12901 atataggaca agttataaat gaaatctcct atgtaaaaaa tgaacttatg gataaaaatg
    12961 aatttaattc agaagtatta acaaaagatg agtttttaaa agtatacaac ttatacgaag
    13021 aacaaaaatc taaagttaac aaaatagatt ttgatgatat gcttataaga acttattatt
    13081 tacttttgaa caataagtct gcactagaaa tggttagaaa tgtgtataaa tatatattga
    13141 tagatgaatt tcaagatata aataaagtac agtttgaagt tttaaagtta atttgtagcc
    13201 cattaaacaa tatatttgca gttggagatg aagaccaaag tatatatgga tttagaggtg
    13261 ctagaccaga ttttttgctt gaatttgaac aatactttaa taatactaaa aaaataatat
    13321 tagatataaa ttataggtct aaaagtgaga tagtgtatac tgcaaataga cttatcgaca
    13381 aaaataaaaa tagatatgaa aaagtcatta agtgtagtca gggggatgga ggaagtgtta
    13441 cctacatttc tccacatgat tcagaggaag aagccttata tattgccaag gaaataattg
    13501 acgaaataca aaaagattat gtagagtatt cagattttgc agtaatttat agaacaaata
    13561 tacagtcaag agcattagta gatgtattta tggatatgag gattccattt gtagtaaaag
    13621 actcagtaat tacaatatat gaccattggg caagtcaaga tatactagct tatttaagaa
    13681 taggtattaa tccaaaatca aataaagatt ggttgagaat tataaacaag ccttttagat
    13741 atatatccaa agacagtata aatatggtta aggatgaaaa agactttata acagcattaa
    13801 taaataagtg taatcttcat cctaaacaag ttaaaacaat aaatgatttg gaaatagatt
    13861 taagttattt aaatacttta aatcctaaaa atgcaatatc ttatattaga actagtcttg
    13921 attatgatag atacgtttta gattattgta gcaacagaaa gataaaaact aatggacttg
    13981 tagagatatt aaatgaactt gagagttcag caactaattt taatactgtt acagagtttt
    14041 tagagcatat tgatagagta aagactgagt taacggaaaa taacaagaat aagcagactg
    14101 agggtgtcat atttacaact atgcatagtg caaaaggatt agaatttaga aatgtttata
    14161 ttataggtgt aaatgaagga acaatacctc atgaaaaatc atatgatatt tgtaaggaag
    14221 agaaaaaaga agaacaatta gaagaagaaa gaagacttat gtatgttgct attacaagag
    14281 ctgaagaaaa gttatgtata agctctactt taaataagta tggtaagaaa gttgataaat
    14341 cattatttat taatgatata aaatctccaa cgaaaaaaga aattgacagt attggtgtag
    14401 gggataaggt ataccataaa aaatttcatg aaggagaaat aattaaaaaa gatggaatta
    14461 tgtttacaat aaagtttaaa gatagagagc gaatcttgga tttgaaaact tgtttattga
    14521 agaatataat ttatactatt taatttagaa tctaaattta gatagaaata taatgtatta
    14581 tttattatgt gcaagacaaa tagatatgtg taaaatgtat tatttaactt attagaaaaa
    14641 gaaataagga tttaatatat tatttatagt tgtttagaaa aagcaacaat gtggaaaata
    14701 ttatttagta ttattaaaga aaagcaacaa tgtgaaagat attatttagt gtaatttaag
    14761 aaaaagcaac aatgtgaaag atattattta gtattattaa agaaagagca acagtgtgaa
    14821 agatactatt tagtattatt aaagaaaaag caacagtgtg aaagatatta tttagtgtaa
    14881 tttaagaaaa gcaacaatgt gaaagatatt atttagtatt gtttaagaaa aagcaacaat
    14941 gtgaaagata ttatttagta ttatttaaga aatagctaaa aatatgaaat atactattta
    15001 taattacaaa aataatatca ataaatataa acagtagtat ttagataatt tacaagtaaa
    15061 aattattaaa aaaataaaaa acttattgac aaaaatatta taaggaagta caattgtatt
    15121 aacaatttaa ggaaacaaat ccaatgatag agaaagtagc atatatggat ttacagcgag
    15181 ttaggggttg gtgtaagcct agcaatgaag tctatgtgaa gagagctctg gaggagatta
    15241 tcagaaactt tagtatgata attggtttac ccgccttaag ggattgagag gataagtact
    15301 tacttatcaa aaagagtggt aacgcggata taattcgtct cttagctgta aagctaaggg
    15361 acttttttga tttaaaaata aaaatatcca tacagcttaa atgctaacag tcacaaattt
    15421 tgaaagtaat ataagtattt atcaataaga gtggtaacgc ggtaaagtcg tctcttagtc
    15481 attaaagact gagagacttt ttttgtaact cgaaacattt aagtattaaa atctgaggga
    15541 aattataatt aaaagagagg ggtagttaaa atgaaagtat ttaaaaaatt attaagttta
    15601 ggattagtat taggattgac attatcgcta gtaggatgta gtggtggagg agaaaaaaca
    15661 aagttagaac aaataaaaga caatggaaaa ttagttgttg gtactagtgc agaatttcct
    15721 ccatttgaat ttcataaggt agtagatgga aaagattcaa taaaaggatt cgatataatg
    15781 ttggctgaag aatttgcaaa agagcttggt gtaaaggttg aaattaagga tatgtcattt
    15841 gatggattaa taggtgcttt aaatgcagac caagttgata ttgttcttgc aggtatgtct
    15901 ccaacaccag agagagaaaa gagtgttgat ttttcagaat tatattattt aagtagaaat
    15961 gcagttatag ttaaagatgc agatatagac aaagtaaaaa ctgaagatga tttaaagaaa
    16021 cttagagttg gagttcaagc aggaagtatc caagaagaat atgtagttaa cactttaaaa
    16081 atgacaacta caaaatcttt aaaagcaata cctgatttaa ttacagaatt aaaaaatgga
    16141 aacatagatg cagttgttac taatgaagca gtttctttaa taaatgttaa aaaatatgat
    16201 ggaataaaaa tggcaaatac tgaagttggt aaagatgtaa cagaaggtat ggcagcagca
    16261 atcaaaaaat ctgacaataa caaagatttt atagaattat taaacaaaaa gataaaagaa
    16321 ttacaagatg gtaagaagat agaagaattt ttaaatgaag catctactga agctgcttca
    16381 aattagtaag aaagttatgt gggagatgaa attatgagtt tagattttag ttttttaagt
    16441 agatttggga cttctttttt ggaaggaaca ggtgtgacag tatcaatttc tcttgtggca
    16501 ttatgctttg gatttataat aggtataatt atctgtatgg caaaaatatc aaagagcaaa
    16561 gtattaagag caatatcatc aatctatatt gaggttttaa gagggacacc attattagta
    16621 cagatatata tagtatggtt tggtttacct caattaggaa taagattccc tatgttgttt
    16681 ggtataccaa gtgaatttat agctagtgca tttgcattat cagtaaactc tggtgcatac
    16741 gtggcagaga tacttagatc aggtatacaa tcagttgata atggacaaat ggaagcaagt
    16801 agatcattag gattaaatta ttggtcaact atgagatata taataatacc tcaagctata
    16861 aaaaatatat taccttcatt agcaaatgag tttattacac ttgtaaaaga atcttcaata
    16921 atatctgtaa taggagttgt tgagataatg cgtactgctg atatagtaaa aaatgcagca
    16981 tttagagcat tagagccatt aatagtagca gcagcaattt actttgttat aacatttact
    17041 ctatcaagat tagttggatt attagaaaag aaactatctg tgtctaacta gatgagagca
    17101 tgtttagcat ttagggaaaa catagaatag taaaaaggaa agtgataata tgattacaat
    17161 aaaaaattta agtaaatctt ttggagattt aaatgtatta aaaaatatag acttagaaat
    17221 cgctaaaggc gaaataatgg ttatagtagg tccaagtggt tctggtaaaa gtacacttct
    17281 tagatgtatg aatttactag aaatacctac aggtggagaa ataatatttg aaggaaaaaa
    17341 tttggtcgat aaaaaaacta atattgatga ggttagacaa aatataggta tggtgtttca
    17401 aaactttaat ttattcccac ataagactat tttagataat ataacattag caccaattaa
    17461 attaaaaaag atgactaagg aagaagcaga aaaaaaagca gaaattttgt tgagtagagt
    17521 tggtctttta gataaaaaag attcatatcc atcacaatta tctggaggac aaaagcaaag
    17581 aatagcaata gctagagcac ttgctatgga accagatatg atgttgtttg atgagccaac
    17641 atctgctctt gacccagaaa tggtaaacga agttttggat gttataaaag aacttgctaa
    17701 agaaggtatg acaatggcaa ttgtaactca tgagatggga tttgctaagg aagttgcaga
    17761 tagagttatt tttatagatg gaggaagtat acttgaagat aatactccag aagaagtttt
    17821 cggaaatcca aaacatgaaa gaacaaaagc atttttagca aaagtgctat aatatataaa
    17881 aaggatgtta ttgatattag aaattcaata catccttttt tagtgatggt taatattaat
    17941 tttaaaatta aagttatatt aatattaact ttatttttac gattttttgc agaaaaaaaa
    18001 agagctcggg gtaagctctt tttttccacc gaattaattt ccgttactaa tccgattttt
    18061 aataaatata aaatatatcc ttatgaaagg gtattgggaa tagatatatt aactaactag
    18121 ggttaccagg gggataacct taatttatta taacaaaatg cgacacaaaa atcaatattt
    18181 tttttgaaat aaattataaa agttattttt ataacaagta agcttataaa tttaactttt
    18241 atagtaaaaa taagtatatg tataatataa tatatttatt ttatataata tattatttta
    18301 tcatatattg tatttta
//
LOCUS       DAELSY010000076        18107 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.076, whole genome shotgun sequence.
ACCESSION   DAELSY010000076 DAELSY010000000
VERSION     DAELSY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18107
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.076"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(47..898)
                     /gene="gatY"
                     /locus_tag="KPG09_002275"
     CDS             complement(47..898)
                     /gene="gatY"
                     /locus_tag="KPG09_002275"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG0966882.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(947..2116)
                     /locus_tag="KPG09_002276"
     CDS             complement(947..2116)
                     /locus_tag="KPG09_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0966883.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     NRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPQGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            2578..3495
                     /locus_tag="KPG09_002277"
     CDS             2578..3495
                     /locus_tag="KPG09_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG0966884.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     ICSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVNQ
                     GEELIELSKKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKHFSVNTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(3624..4553)
                     /gene="pfkB"
                     /locus_tag="KPG09_002278"
     CDS             complement(3624..4553)
                     /gene="pfkB"
                     /locus_tag="KPG09_002278"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG0966885.1"
                     /translation="MITVITFNPSIDRMYKVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEYNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            4822..5547
                     /locus_tag="KPG09_002279"
     CDS             4822..5547
                     /locus_tag="KPG09_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0966886.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRIQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            5630..6784
                     /gene="nagA"
                     /locus_tag="KPG09_002280"
     CDS             5630..6784
                     /gene="nagA"
                     /locus_tag="KPG09_002280"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG0966887.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNITDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQRKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFSVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNIATLEDAIKM
                     ATYVPALSCNIDNVCGSIHQGMDADFIVTDKNFNIYETYISGICMYKVNK"
     gene            complement(7144..8358)
                     /locus_tag="KPG09_002281"
     CDS             complement(7144..8358)
                     /locus_tag="KPG09_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBG0966888.1"
                     /translation="MFNKNEILKKLIEEYDVKTTTDIQDMLKDLFASTIHQMLEAELD
                     DHLGYDRYDNKNKNTKNSRNRYRNKNVKSDFGEITLNIPRDRNGDFEPKVIRNYENDI
                     SGIENQVIGMYSKGMSTRDISSHLKSIYGIDISHSLIAKITDRVLPLAHEWQSRALDM
                     VYPIVFMGAIHYKVRSEGRIINKAAYIAIGVNLEGITEVLGIWIGENESSKYWLKVLT
                     DVKNRGVKDILITSVDGLVGFSDAIKAVFPDTEIQRCVVHQIRNTLNYVSYKNRKEFA
                     KDLKKVYAAITEEVALSELSLLEEKWGNQYAIALRSWRSNWNELATYFKYPQEIRTLI
                     YTTNSMESYNRLLRKVTKSKSIFPSDDSLHKSLYLATMDISERWTQKIRNWTQILAHL
                     SIYFEERILTLN"
     gene            complement(8545..9702)
                     /locus_tag="KPG09_002282"
     CDS             complement(8545..9702)
                     /locus_tag="KPG09_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBG0966889.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            9856..10425
                     /locus_tag="KPG09_002283"
     CDS             9856..10425
                     /locus_tag="KPG09_002283"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBG0966890.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            complement(10607..11023)
                     /locus_tag="KPG09_002284"
     CDS             complement(10607..11023)
                     /locus_tag="KPG09_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966891.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(11249..12343)
                     /locus_tag="KPG09_002285"
     CDS             complement(11249..12343)
                     /locus_tag="KPG09_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966892.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            complement(12766..13707)
                     /locus_tag="KPG09_002286"
     CDS             complement(12766..13707)
                     /locus_tag="KPG09_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG0966893.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGINAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
     gene            complement(13700..14527)
                     /locus_tag="KPG09_002287"
     CDS             complement(13700..14527)
                     /locus_tag="KPG09_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG0966894.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            complement(14698..15081)
                     /locus_tag="KPG09_002288"
     CDS             complement(14698..15081)
                     /locus_tag="KPG09_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBG0966895.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            complement(15050..15337)
                     /locus_tag="KPG09_002289"
     CDS             complement(15050..15337)
                     /locus_tag="KPG09_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBG0966896.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            complement(15370..16527)
                     /gene="alr"
                     /locus_tag="KPG09_002290"
     CDS             complement(15370..16527)
                     /gene="alr"
                     /locus_tag="KPG09_002290"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG0966897.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            complement(16542..17132)
                     /locus_tag="KPG09_002291"
     CDS             complement(16542..17132)
                     /locus_tag="KPG09_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966898.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            complement(17153..17614)
                     /locus_tag="KPG09_002292"
     CDS             complement(17153..17614)
                     /locus_tag="KPG09_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG0966899.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            complement(17629..18009)
                     /locus_tag="KPG09_002293"
     CDS             complement(17629..18009)
                     /locus_tag="KPG09_002293"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBG0966900.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
BASE COUNT         5961 a         3124 c         2096 g         6926 t
ORIGIN      
        1 acaaatatcc taaacttatc taaaataaat ttaggatatt ttatttttat actctattta
       61 cagacccaca cactttaatc ttttctttta caaaatcctt cactgattga agccctgcta
      121 atccatactt tttagggtct ataacctcag aatcttcatc taaaactttt ttaacccctt
      181 tactatatgc aattcttaat tcagttgcat agttaacttt tgcaatacct ctttttatag
      241 cttcttttat aatatcatca tgtattcctg aacctccatg caatactaaa ggtattgaaa
      301 ctacattttt aatttctgat aacctatcta aatctatttt aggttcaccc ttatacaatc
      361 catgtgcagt acctatagca atagccaatg aagatattcc tgttctatcg acaaactctt
      421 ttgcctcttt tggatttgta taatcattac tattcccgcc atctaaatca tcttcttttc
      481 caccaacttt cccaagttct gcttccactg gtataagaga aggactacat gcttttacta
      541 ctgatttaga tatagatata ttattttcaa agctttcatg agaaccatct atcattattg
      601 atgtatatcc acttcttaaa gctttcattg caaggtcaaa acttgaacca tggtccagat
      661 gcattgctac aggtatcttt actctttctg cagcaacctt tgcatttgct agataataat
      721 caagacctgc atattttaca gtagatggag tagtttgtaa tattactgga gagtttaact
      781 cctcagctgc ttctataaca gcaattacca tttccatatt ttctacatta aatgctccta
      841 ctgcataccc ctcatcttgt gcctttttaa gcattgatgt tgttgttact aatgccacat
      901 tgataccctc ccaaattatt tagttataat attttaatct tatcttttaa tattcatata
      961 ttgttacacc ttttactact ctatttactg ttcctgtagc tgatggcgta tctggcttat
     1021 ttccaacctt taccgataca aacaatgcta tagtttgtgc aaacaatatg tatggcattg
     1081 caagatacac atctggtaat ttattatact tttctttaaa ctcaaacttt gttcctgaga
     1141 agttattttc attttctact gacactgcac aagtgaattt agcaatttta tcccttttta
     1201 tctcctctaa aacatctaaa tcatattttc ttgtatataa acagttagat acaaattcaa
     1261 atacaagtgt attttcatca ataaatgatt ttggtccgtg tctaaacccc ataggagaat
     1321 catacaccgt agatattttt cctgctgtaa gttctaaaag ttttagttgt gcttcttgtg
     1381 tcaaaccacc taatcctcct gaacctaaat atacaactct atcgaaatct ttatttatta
     1441 actcatgtat ttcatctttt ctatctataa catttcttcc catttcaatt attgcattta
     1501 tatatgactt ttctacatca tcttctaaac tatcgaatat taacatagca gataacatca
     1561 tacacgaaaa acttcctgtc atagcaaagc cttcatcatt tgattttgat ggcattaata
     1621 acaagtaatt attttcgtcg tttttagtca ttttagctag ttctccttgc ggattacaag
     1681 ttatagctaa atgataaaaa ttatctacaa tcttattccc taaatttaaa gcagccaaac
     1741 tttcaggact atttccactt cttgcaaatg aaattaataa tgttggtata tcttttttta
     1801 agtaatcata tgggtttgaa actatatccg tagtaggtat tgcttcaaat atgtattttc
     1861 ttatgtcatt tttattctta aggtaaggaa gtattgaatt tcctatgtaa gctgaagttc
     1921 cagcacctgt aaatataact ctaaactgcc catgattatt acttattgta tctatgaatc
     1981 tatttaattt ttctttgtta gatttatata tttcataagt ctctaaccat aactcaggtt
     2041 gttgttttat ttcattagtt gttattattg cacctaaatc ttttagtttt tgttcttcta
     2101 atttaaacat tttcattctc ctttcatact tgtaacgttt acatttggta atgaggttat
     2161 aaccacttta aactataaaa aaatatactc ttttataatt ttttgcttag attttttagt
     2221 actacttaaa ctcactataa aatatacgct atagatttta attaatttta tagtttatct
     2281 ttaaaaattt aataaaatca tactttagta tttttatttt acgataaaaa cttgttcaga
     2341 ccaaagtggt aattaccact aatccaaata taaattaatt tgaatttttt gtcaatacat
     2401 aaaatattta tatcaatcaa ttttatagat atcctacaaa attactttgt caatatttct
     2461 tatttcgtta atttacttat ataagcttta aatttatttt ttgatacaaa gtttacaaat
     2521 ttatgtgtct gaattataat caattctgta aatataaaaa ccttaggaga aagtattatg
     2581 aaaaatataa aaatgggaat gataggtctt ggctccatag ctcaaaaggc atatttacct
     2641 atattaacta agtctgagag atttgagttt gtaggagctt ttactccaaa taaagttaaa
     2701 agggaaaaaa tctgtagtga ttatagaatt atgccattcg attctattga atccctagct
     2761 aaaaaatgtg attgtatttt tctacatagc tctacagaaa cacactacga aattataaaa
     2821 atattattaa atttaggtgt tcatgtatat gtagacaaac ctcttgcttc aacagtaaat
     2881 caaggagaag aacttatcga gttaagtaag aaaaaaaact taaatttaat ggttggattc
     2941 aaccgtagat tctgtcctat gtacaaagaa attaaaaata atgctacaga aatcgtatct
     3001 atcaatatat gtaaacatgg attaaattcc ttaagaaatg ttagattcga cagtaccctt
     3061 atagatgact atatacatgt tattgacact gctttatggt tagcaaatga agatgttgaa
     3121 attagcggag aagatttatt tttaacagac aataagaatc ttatatttgt aagtcataaa
     3181 cttaaaggta aacatttctc tgtaaacacc tctatgcata gagattctgg aacaaagtta
     3241 gaacaagttg aaattctttc aaaaggtaag attcaaagag ttaagaatct taatgtactg
     3301 gaaatagaag aaggtggaaa cctaacttta aaacaaagtg gtgcatgggt aaatatatta
     3361 aaacaaaagg gttttgagga tatatcaaat cattttattg attgtataga aaataatata
     3421 aaaccagcta ttaatggaga agagtgtatc aaggctcaaa gattactaga gaaaattata
     3481 aattcagtaa aatagatatt tattataaaa ataggttaaa aatgaatttt tctaacctat
     3541 ttttatataa gactatatct tttatattaa aattaaaatc tattacaaca acattaaatt
     3601 tattatattc ccattaaatt tatttaactt aactttttta cttcaactaa atcttgatat
     3661 ttatcaattt ctcccaaatc tacaaatcca gtttccatat ttaaagcatt acttataccg
     3721 caagcatttg caaatttaag taaattttct attttataac cccttaaaat acctacacta
     3781 aatccagcca ctgtgctatc tccactacct acagtattta tacactttac aactggtact
     3841 tttacatcat atacattata ttcgtttaaa tacaccatac catcttttcc taaagatata
     3901 catacattct ctgcacccat ttcaataagt ttttttcctg acaagataat ttcctctctt
     3961 gattctatat ttattcctaa taaatgtctt atttcatcta tatttggctt tattaaatat
     4021 ggttttgatt ttaaagctat ttttaatgct tctcctgatg tatctaatat aaattttata
     4081 ttatttgctc tacatatatt tcctatcttt tcataataat ctatcggcat attctcacaa
     4141 taactaccag aagctactag tattttagta ttttttagta cttcctctat atcattttca
     4201 aatctctcta aatcgctttt ttctactatt ggtccttttt ctaaaaattc tgtactaact
     4261 ttattgtcct caattatgtt taagcaattt ctagtttcct gctctatttt tgtaaattta
     4321 ttttgtatat ctagttttct aagttcattt tttataaatt ctccattaaa tccacctaga
     4381 aatcccatag ctaaaggatt ttcttttaaa attttacata ccttagctac gtttaatcct
     4441 ttacctccag cagttgcatt cttacttact accctttgca cctcacctat gtttatatta
     4501 ttcactttat acattctatc tatagatggg ttgaatgtta ttactgtaat catactagat
     4561 tctcctcatt attaattttt acttttctaa acaataaatt aatctgtaaa ataaaaaata
     4621 cctcatctat aatataccca aagtggttat taccaatttg aatataaatt attttacttg
     4681 ttttgtcaat ataccataat tttataataa attaatagcc ttacttattg aattttgatt
     4741 aaactttatt cattgacata aataattcta tattctataa tcaaattgtg gtaataacca
     4801 cttagaaagg actatgtttt tatgataaaa agaaatgata aaagaccaat atatgaccag
     4861 cttgttgaga tattaagaac taagattgaa aatgagatgg aaccaaacga tagaatgctt
     4921 tctgaaagaa aaatttgtga tgaatatgga gtaagtagaa ccacagttag actagctatg
     4981 gctgagttgg aacatatggg atatatatac aaaagacatg gaaaaggaac ttttgttgct
     5041 gcactaagtc aaaattctca aaacttaatg gaaagttata gctttacaga ttatatgaga
     5101 atacaaggaa aaaagccagg tacaaaggta ttatcatttg aggtagtaga aagtacaaag
     5161 tatttctctg aaaaattagg tataactcct ggtgaaaaaa tgataaaaat aatgcgttta
     5221 agacttgctg atgaccttcc tatgatgctt gaaagaacat atcttcctat gaaagaattt
     5281 ataggattga caaaagaaaa aatcgaacaa aaacctttat atgaaatatt tagagaagat
     5341 tatggcgaga ttattaaagt tgctgatgaa gaattttcag ctggaataat ctctgataag
     5401 gaagctaagt tattggaagt accaggagat tcaccttgtc tgaaattact tcgtaccaca
     5461 tataatgatg acaatatagt tattgaattt actttgagtg ttgctagaag cgataaattt
     5521 gtatatagag taagacatgt aagatagtga attaaatagc tttcaagaaa atagaatgat
     5581 attataaacg tcaatgccac aatgatagta taattataag gaggttattt tgaaaagttt
     5641 tatttatgca gataaatttt ttatgaaaca tggaatcaaa gaaaaaggtt accttagtat
     5701 aattaatggc aaattcggtg aatttcaaca agaaagacct aatattactg ataatgtcat
     5761 agatttttca ggtaaatata ttgctcctgg cttagttgat actcacattc acggtctttt
     5821 aggtgcagat gtcatggaca atacatttga agcaataaac acaatttcaa aaggcttatt
     5881 aaaatatgga gtaacatctt tcctacctac aactttaact gattctgtag atacactaag
     5941 tgattctgta gaaaatataa gcaatagctt caatcgagtt gagggtgcaa aaatacaagg
     6001 gatttattta gaaggtccat tttttacaga aaaatataag ggcgctcaaa atgaaaaata
     6061 ttttaaagac cctgacatca atatattaaa attatggcaa agaaaatcga aaaatttaat
     6121 aagaaaaata gcaattgctc cagaacgtaa aggttcaata gaattaacaa gatatgcaac
     6181 taaaaataat atctctgttt ctcttggcca tagttcagct acatttgaac aagttaaaca
     6241 agtcgttaat tcaggtgcta aagtgtttgt acatacatat aatgggatga gccctttaaa
     6301 tcatagagaa cctggaatgg ttggggctgc tatggtacta aaagatactt atgctgaatt
     6361 aatttgtgat ggtcatcatg taagcccaat agctgttaaa ataatgatgg atgcgaagtc
     6421 tagtaataac atagctttaa ttacagattg tatgagggct ggtgctatgg aggatggaaa
     6481 atatactctt ggtaagttta gtgtaaatgt aaaaaatagt gttgcaagat taaatagtgg
     6541 gagtttagct ggaagtgtat taactatgga caaggctgtg agaaatatag ttgattggaa
     6601 catagccact ttagaagatg caataaaaat ggctacatat gttcctgctt taagttgtaa
     6661 tattgataat gtctgtggct ccatacatca aggtatggat gcagacttta tagtaacaga
     6721 taagaacttt aatatatacg aaacttatat aagtggtata tgtatgtaca aagttaataa
     6781 atagattcaa tataaagtta tggaggcttc ctaaaaatag agataagtct ctttttcatt
     6841 gttaaaatga tgaaaataca tttgatttca tacaaaaaga gtgcctcata aactaaaaag
     6901 ttaattttga gacactcctt ttattaaggt tctttatttt acaatataag tttataaaat
     6961 taagaatctg gaaatattta tgtgtattct ttgaataaaa atttattttt tagtgataat
     7021 taaacattta ttggtaaggc tatggcttta aaaatcatag ccttattttg atattttttc
     7081 catttttgta aaaattatta tccctttaag aaaatttctt aatttttttg aaatatttct
     7141 ttattaattt agagttaaaa ttctttcttc aaaataaata cttaaatgag ctaagatttg
     7201 agtccaattt ctaatctttt gagtccatct ttcagaaata tccatagttg ctaagtataa
     7261 tgatttgtgc aaagaatcat ctgaaggaaa aatagacttc gatttagtaa cttttcttaa
     7321 taatctattg taactttcca ttgaatttgt agtatatatc aatgttctta tctcttgagg
     7381 atatttaaag tatgttgcta gctcattcca attacttcgc caacttctta atgctatcgc
     7441 atattgatta ccccattttt cctctaagag cgatagctca cttaatgcta cttcctctgt
     7501 aattgcagca tatactttct ttaaatcttt tgcaaattct tttctatttt tatatgatac
     7561 ataatttaaa gtatttctaa tttgatgtac aacacatctt tgtatttcag tatcaggaaa
     7621 tactgcttta attgcatcag aaaatccaac taatccatct acagaagtta ttaatatatc
     7681 cttaactcct ctgtttttga catctgtaag cacctttagc caatactttg aactttcatt
     7741 ttctccaatc catattccta agacctccgt tataccttct aagttaacac ctatggctat
     7801 atatgcagct ttgtttatta ttctaccttc acttctaact ttataatgta tagcacccat
     7861 aaatacaatt ggataaacca tatcaagagc tctgctttgc cattcatgag ctaaaggtag
     7921 aactctatca gtaattttag ctattagaga atgagatata tcaattccgt aaatactctt
     7981 aaggtggctt gatatatctc gagtagacat accttttgag tacattccaa tcacttggtt
     8041 ctctattcct gatatatcat tttcataatt tctaattacc tttggttcaa aatctccatt
     8101 tcgatctctt ggaatattta aagttatttc tccaaaatct gatttaacat ttttatttct
     8161 atatctattt ctagagtttt tagtattttt attcttattg tcatatctat cataacctaa
     8221 atggtcgtca agctccgctt ctaacatttg atgaattgta cttgcaaata aatcttttag
     8281 catatcttga atatctgtag ttgttttaac atcatattct tcaataagtt tttttaatat
     8341 ttcgtttttg ttaaacataa aaagtgtgca ccttccttat atatagtgat atttaatatt
     8401 ttaattatca ctacttttta gaagaataca cactttattt taaagaccca caaaagagtg
     8461 cctcataaac taaaaagtta attttgagac actcctttta ttaaggttct ttattttaca
     8521 atataagttt ataaaaatta aactttattt atcaatctgt tctctaagcc atttagtcgc
     8581 tacatctaac tgtttatttt catctttttc ttcaatagta ggctttatgc ctttttcatt
     8641 gatataatct ccattaggtg taaaatattg agcagtagtt aacttgtatc ctgttccatc
     8701 cttcaatctt acaactgatt gtaccaaccc tttaccaaaa gttgtagtcc ctacagaaat
     8761 accttcttta ttatcaacga ttgcagctgt taatatttca gcagcagaag cactttctcc
     8821 attagttaac actactattg gaagtcccaa tttttcttta tcagatttca agtactcagt
     8881 atttccctta ttatcttttg tatatactat agttccttca ccaattaact catcagcaac
     8941 ttctttacat atatctagca ttccaccagg attatttcta aggtctatta ctaatccctt
     9001 gatatttttt gcttttaatt cttttaaagc tttatcaaag tcagtatatg tacttgatat
     9061 aaattgggta atttctatat atcctaaatc attatcaatt actttacttt ctatactctt
     9121 ctcaattatc tggtctgttt ttactttaaa gttcaattgt ttatcttctc ttaatatagt
     9181 cagttcaact gttttccctt ttttaccctt catcatacga acagcttcat ctgaattttt
     9241 atatgagaca gattttccat tgacttttac taatttatcc cctgatttta cacctgcttt
     9301 ctctgctgga gaatctttca tgggaactac tactaagcca ccatcttttg atgcccctat
     9361 atacatacct acaccaacat aagaaccttc actatcatta attagattct ccatttcatc
     9421 tttagtataa taaccagaat acttatcatt tgttcctaga aatagacctt ttgctgctcc
     9481 atttaccaaa tcagtatctg atactttttt ataaaaatct tgttttataa tagattctag
     9541 tcctaacaac ttatcatatt ttttatagtc ttcatagagt tctttcgata ttacaacttt
     9601 attccccaat gtcaactgaa atgctgatgt aaccatagct gttataatta ctaaaatcac
     9661 ccctaaaaaa atagctttct tctttgaaat cacaagatac atctcctttt acataaacta
     9721 ttattattat atgactagct gttattttta ttacatttac ccaaatatat tgctttatta
     9781 caataaatta tactaaaatt tatattaaat acaattgctg tacacattat aacatattaa
     9841 gggaggaatt attttatgaa aaaagaattt agaaaaaaag ttattgaatc aagaaaaaat
     9901 aagagtgcaa actttgtact tagtaattca aatttaataa ctgaaaaatt acttcaaatg
     9961 gaagatataa gaaatgcaac taatataatg ctttatctaa attttaacaa tgaagttcaa
    10021 acagataatt taatcaaaaa acttttgtct ctaaagaaaa tagtatcaag tcccatcaca
    10081 ataaaagaaa atcatacatt aatccctact caaataacag atttaaaaga gggattgaga
    10141 attggagcat atggaataag agaacctaac gaaaaatcac ctacaattga tattaaggcc
    10201 ttagatgttg taattgtacc agctgtagca tatgatatac attgctatag attaggatat
    10261 ggtggaggat tttatgatag atttttagaa aacttaagaa aagatgctat aactattggc
    10321 atagcatttg acttccagat attcgattct attccaaaag aaactcatga tgcacaatta
    10381 gactacatag ttactgaaac tagaattatc actccaaata attagaacat taagtatttc
    10441 cagaccccat ttaatatagt aataaatact actgttaata tcttacttta taagcatttt
    10501 aagtcatggc ttttacaaca ataagaacca tatacaattt acttacatta ataaaaacca
    10561 tgactttatt gtacaatatg cagttattta attataaaat accttcttat ttcgtcgcat
    10621 ttaattctat tctatctgga agttcactca tatttgtttc aacaactatg tatggaaaag
    10681 aatcttcctt atctttatac tttatcttga ctctcaaaac atcctttcct tcctcaacca
    10741 cactttctat tttgtccatt tctataccat atttattatt tcctctagtc actataatgt
    10801 atattttgtt atcgatttta gccgctaatg ctctttcttc atttgtatac ttatccatca
    10861 catctaatat cttttctggt attgaatttc tttgtaatac tgtataatct atgggtttag
    10921 atttctctac aaacatactt ggtatgaata taaatccccc tataacgaat acaagagcta
    10981 taactccata aattatacct ttaatattaa attttacatt caatcttatc actcctccca
    11041 tatattctaa atatatgatt tataagctta tcttagactc taaattattg attagtatat
    11101 atttatccat aattataaat atatgtaatg taactaaaaa acatgttttt cctatttcat
    11161 aataaacgag ctaaaaaata aaaagagcta tatgaaaaca ttttcattgt tttcatatag
    11221 ctcttgagtt tacaatatta aaaaatactt atttgtcact gattccttct tgatatccag
    11281 atgtttttgt aatctgtcta taaagaacac aaaccagtat tatgacgccc atatatccag
    11341 tatatggata taaaatacca accagcttat caaatggtag gaagccacct ataagaccta
    11401 atatagctgc tattaaagtt attaatttaa acttaggatg tttatcatct acaaatctat
    11461 tagttactga ccataataaa ggtacagctg ttgtatatat acctaatata agaattattg
    11521 agaataaaac tccaattact ggtgaaatct tatctgctaa gaataatgat ggtattgctt
    11581 ttgaatataa gtttcctata tcagaaagaa tagctaagtg catcattata gctgcagcca
    11641 taagtgctac tccacctaat ataccaccac aaatagcttc ttttttgtta gcagcacttg
    11701 ctcctaatcc agttaagaat gccataacaa caattgtgtt ataagtagta tatattacac
    11761 cagatagata ggcatttgga actggtttag ctatctttac agtgctaaga acttgtgcag
    11821 cttctcctat attacctatg tttttagcta aacttatacc accaactaaa agtgtaaaga
    11881 ttattattat aggccctatt ccacctagta tctttgataa tttatcaaga cctagtgaaa
    11941 cagttaataa tgctactact gccataccta ctctacctac aaatggattt aatccatagt
    12001 attcagttaa agttgctcct gctcctgaaa tcattactac gaaaacacca aataagaata
    12061 aaggtccaaa ccattcaaag aatatcccta aatacttacc acaataaaca cgataaattt
    12121 tgataggttc tttaagttga agctctttac cttttgacat aactgaagca cccatccatg
    12181 agaataatac cattgaaatt atcccaccaa taatacctgc aaaaccataa aaagagaaga
    12241 attgcattat ttcctgtcct gttgcaaatc cagaaccgat tactgttgct acatacgcac
    12301 ctgcgaaact gatgactgtt ttaacattta ctttttctga catatctcag ttacctcccc
    12361 atgtgaaatt ttttcttctg attaaataat tcgataaact acctacaatt tcctttaaca
    12421 taaaatacat gataactgtt ttatagcttt ttagtataat aacttaaaac ttataatctg
    12481 attttatacc ctactcctcc tctcataatt tttttagatt cttttatcta agtagtcatt
    12541 atatataaaa ttagtttctc tatagattat atattagtca tatttttctg aatagtcaat
    12601 atatatttga atattttctc tatttttgta aaaatttgaa tattttatta catttttgat
    12661 atattgatat catataaaaa aaagagagat ataaattaat ataacacttt atacctctct
    12721 tttaaatata ttatattatt aacaaacatg aataatttat atctcttaca ttctttttgc
    12781 aataatagct tctttaacag tctttattat gtcatcacaa gttaattcat atttttttaa
    12841 caattcattt ggagtaccag attctccaaa tgtatcttta attccaactt tctttacaat
    12901 ggttggacaa gactctccta ctacctcact tacagcagaa cctaatcccc caattatgct
    12961 atgttcttct acagttacaa tagcattcgt ttcttttgct gattctaata ttaaatcttt
    13021 gtctataggt tttatcgagg acatgtttat aactctagca tttatacctt cttcttgcaa
    13081 cttttcttgt gctaatagag cttcatttac cataattcca caagctataa ttgatacatc
    13141 ctttccttct cttaagactg taccttttcc tatttcaaat ttataatctt catcaaataa
    13201 aactggtact gaacttctcc ctaatctaac ataaacaggt ccattatact tagctatttc
    13261 aaaaataatt ttttctgttt caactccatc agctggaaca agaactgtca tatttggtat
    13321 agctctcatt atagcaatat cttccacact ttcatgagat gctccatcct ctccaacagt
    13381 aagacctgca tgagtagcac atatttttac atttagcttt ggataacaaa ctgaatttct
    13441 tataatctca aaagcccttc ccgtagcaaa catagcaaaa gtacttgcaa aaggtatttt
    13501 accagcagtt gataatccac aagcagcgcc tattaaattt tgttcagcta tacccatatt
    13561 aaaaaatcta tctggaaatg acttgtaaaa atcatgtgtt tttgtagact ttgataaatc
    13621 tgcatctaat actacaacat tgtcatttat ttgacctaat tttactaatg ctttaccata
    13681 tgcttctctc gtcgccattt tactcattgt cagcacctcc taattcaagc aaggcttttt
    13741 ctagctcctc atcacttgga gctgtcccat gccatcctgc ttgattttcc ataaaagata
    13801 ttcctttgcc ttttatagtt tttgcaacta tcattgtagg ttttttaact gttgatttag
    13861 ctatatcaag agccgcaaat atttggtcaa aatcatgtcc atctatctca attacattcc
    13921 aaccaaaagc tttaaattta tcaactattg aacctaaact cattacgctt tctatatctc
    13981 catctatttg aagtccatta ttatctaaaa aagcaattaa attatcaagt ttataatgtg
    14041 cagcactcat agcagcttcc caaacaattc cttcttgaac ctcaccatca ccaagcaaag
    14101 tatacacatt ccacggagca ttatccaact ttgatgccat agccatacca catgctactg
    14161 aaaaaccttg acctaaagaa ccagtagata tttccacacc tggcgtgcct ttcatgtctg
    14221 gatgaccttg taacatcctt cctatcttcc ttaatccatt cagttcttcc ttatcaaaat
    14281 agcctttttc agccaatgta gcatataatg ctggtgcagc atgtcctttt gataatacaa
    14341 atctatctct atcctccatt ttaggatttg aagaatccac attcatttca tcaaagtata
    14401 acgcagtaag tatttcaact actgataatg accctcctgg atgtccagat ttggctctat
    14461 gtatcattga aactatatct cttcttatga tacgcgcaat ctcatttaat cctttatggt
    14521 ctctcatgca attacctccc ttgagttcgt aattttatat caattccata ttattatatc
    14581 acttttatgc tatttttttg cgttattttt aattttatta acatagtaca ttaaaattaa
    14641 accttaagag atatttaaaa gaaaaaaggg attgtctaat aaaagacaaa tccctcttta
    14701 aaaagtaaac aatcctaaac ttatttgaag tccatcatct acttttatca tcatattttc
    14761 atctagacaa ccaatttttt ccctcagtct ctttttatcg atagttctaa tttgctctaa
    14821 aagaattact gagtctttat taagcccata ttcattggaa cttatctcta catgagtagg
    14881 taatttggct ttattaatct gagatgttat agctgatact ataactgtag gactatattt
    14941 attacctaca tcattttgta ttatcagaac aggtctaacg cctccctgct ctgaaccaac
    15001 aactggactt aaatcagcgt aatataaatc accacgcttt atttcacaat tagtactcac
    15061 cttgggcaat cttcccttca tttttatcat cagaagatac ttcttcaact gtcataccta
    15121 attcagctag tgacagatta atttgaccca tttctctata acccgttttc atagattctt
    15181 taatatgaat tttctttttc tgagttatat agaactgaaa agcagctttt gcaaaatctt
    15241 ctctgttact gttctccatc tgaactatac tatcaacttc agaaatcaga gagtcaggta
    15301 aagtaacttc tattttttct ttactcttat cgtacacaaa atcgcctccc agattataat
    15361 atcatggttt tattttagca aataactgtt tatttgtaca agctcattat tttccatata
    15421 aacacggtca actcttcttg atatcataca taacacttca tagtttatag ttcctaagtc
    15481 tttagctata cgctcagctg taacttcgcc ttctccaaat agtataacct catctccaac
    15541 ttttatgtct atatctttgt caattctaac cattatttga tccatacata ttctaccaac
    15601 tacatcaaac acttctccct taataagaac ctttggattt ttttggattc tagtaaatcc
    15661 atctgcgtat cctattggaa ctgtagctat tgtttcttta cctgtagttg tgtattttag
    15721 tccataactt attcctacac ctggttcaac ttgttttata tgtccgattt ttgattttaa
    15781 tttcatggct ggtcttaatt ctaatctatc tttaaataca tcatcagatg gataatgacc
    15841 atataatatt attcctgctc ttaccatatt taatcttaaa tcagggcaat ccataatagc
    15901 tgcactgttt gatacatgtt ttatagctat ttttacacca gcctcatcta atttatcaga
    15961 cataaattta taattattag cttgtttata agtgtactct ttacttactt catcagctgt
    16021 agcaaaatga gtaaacatac cttctaaatc gatatattct aatttattta attctattat
    16081 ttcctgtact gactcctcat taggttggaa acctattcta gtcatccctg agtctatttt
    16141 aacatgaaca caggcttttt ctcctaaaga ttttgcaatt tcatttatct tttgtgctgt
    16201 ttctaaagaa taaactgtca tagttatttt attttttata gaatcttcaa aagcttcgtc
    16261 tggagtatat cccaagttca aaataggaag tgttatgcca ttttgtctaa gttcaattcc
    16321 ttcagcagtt cttgctactg ctaagtaatc tactttttct ttttctagca attttgcaac
    16381 ttctactgca ccatgtccat atgcatcagc ttttattact ccacaaatct taatatcttc
    16441 ttctaataaa tttttaatat tatttaagtt aaatcttaag ttatctagat ttatctctgc
    16501 ccatgtaggc actgttattt tttgcatgtc tttagtcccc cttagtttct gtattcaaaa
    16561 tctttgtatt ttactgtaaa tcttggcact ccctctttat ctaacacttc cattttttca
    16621 ggattttttg tgtcagcatt tacatataat acttgcttgt taaaatattt attatcccca
    16681 ggaataaaag tctctaaaac caaatgtcct ttggataact ttactttcat ctcctcattt
    16741 tgaagatagt tttttataaa gtctcctaca aaaagatatt gattattttt tcctgtatta
    16801 ggtaactcca ctacatcact aatctttgga tttttaacta atattttatc accttgatat
    16861 tctatacttt tcccttttaa atgttttggg gaaattactt ctaatttata gttatcggtt
    16921 tttttataag tatgaattaa aacataattg tgtgggcttt tatttccaac tacttctact
    16981 tcagctttac aactgtaata attcatatca gaaatctgct tttgaaaatc tttgtatact
    17041 tcttcttttg tggactgtcg tttttggcat cctattacaa ttataaccag tgccaagaac
    17101 ataatacata ctatggtcca cttttttctc acatcacacc tcctacttca gtttattcat
    17161 tgtataaagc ttttattata tcatatctac aaattatacc tttaagtttg ttgtcatcat
    17221 ctactacagg aactctgttt attgattttt taatcattac attagctaca tcatcaaact
    17281 tatcatcttc atgaaccttt attatatctt tagacataag ttctccaacc ttataagcag
    17341 ctacttgttt tatatctttt tcaacttttt ttaagtcatc taagaaaatc aatccttgta
    17401 atagatttat atagagaggt gcttctatat atttttcttt tttaagaata tctgtttcag
    17461 aaattattcc aacaactcta ttctctgaat ctacaactgg cagacctcct attttttctg
    17521 caattagcat attagctaca tctgctatac tatcatcttg ttttgctact attacatctg
    17581 ttgtcattat ttcttttgct gttttatcca tcataatgta atcactcctt agtccttaat
    17641 tatggttatc gcatttgcta tagcataatc ttttgaatgt gatattgaaa cttttatttc
    17701 taaaacatta taatctatgc acatttttgc tagattatta taagttttta ctattggttt
    17761 tcccatttca tttctgagaa cttctatatc tttaaaatta aacaatctaa ttcctgtacc
    17821 tatagattta cttatagctt ccttggctgc gaaattacct gctatacttt ctgctttaaa
    17881 attcttagaa ttaaaatact ctatttctct atctgtaaat atcttctcca aaaacctatt
    17941 atttttgtct acagcctttc ttattctatc aatttcaatt atatctacgc caatatcaaa
    18001 tatattcatt gttcctccag aaaatttata atacttcttt caataattaa taatgaattt
    18061 ataaatttac tatcgataat acacattatt atttagttta tgttact
//
LOCUS       DAELSY010000077        17809 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.077, whole genome shotgun sequence.
ACCESSION   DAELSY010000077 DAELSY010000000
VERSION     DAELSY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17809
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.077"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            216..392
                     /locus_tag="KPG09_002294"
     CDS             216..392
                     /locus_tag="KPG09_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966901.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     SILEKELNISKANT"
     gene            566..1696
                     /locus_tag="KPG09_002295"
     CDS             566..1696
                     /locus_tag="KPG09_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG0966902.1"
                     /translation="MNNFEIKTRIKFGEGSLKVLQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKSIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSDTAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILNNIK"
     gene            1937..2287
                     /locus_tag="KPG09_002296"
     CDS             1937..2287
                     /locus_tag="KPG09_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG0966903.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKRD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            2295..2744
                     /locus_tag="KPG09_002297"
     CDS             2295..2744
                     /locus_tag="KPG09_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBG0966904.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDDIGLEKLFKYLEKSKNIDEV"
     gene            2846..3421
                     /locus_tag="KPG09_002298"
     CDS             2846..3421
                     /locus_tag="KPG09_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG0966905.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            3414..4823
                     /locus_tag="KPG09_002299"
     CDS             3414..4823
                     /locus_tag="KPG09_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HBG0966906.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSNENGTEVLITIPTQINI"
     gene            5007..6440
                     /gene="eutA"
                     /locus_tag="KPG09_002300"
     CDS             5007..6440
                     /gene="eutA"
                     /locus_tag="KPG09_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBG0966907.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQTIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSVDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            6458..7822
                     /locus_tag="KPG09_002301"
     CDS             6458..7822
                     /locus_tag="KPG09_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBG0966908.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFYKDGKLTELAGDAS
                     VLLG"
     gene            7835..8716
                     /gene="eutC"
                     /locus_tag="KPG09_002302"
     CDS             7835..8716
                     /gene="eutC"
                     /locus_tag="KPG09_002302"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBG0966909.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKTTEVVVDNNAFI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSQETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            8736..9389
                     /gene="eutL"
                     /locus_tag="KPG09_002303"
     CDS             8736..9389
                     /gene="eutL"
                     /locus_tag="KPG09_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBG0966910.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEIAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            9400..10101
                     /locus_tag="KPG09_002304"
     CDS             9400..10101
                     /locus_tag="KPG09_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG0966911.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRIDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEITDNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            10104..11573
                     /locus_tag="KPG09_002305"
     CDS             10104..11573
                     /locus_tag="KPG09_002305"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HBG0966912.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNIPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            11669..11956
                     /gene="eutM"
                     /locus_tag="KPG09_002306"
     CDS             11669..11956
                     /gene="eutM"
                     /locus_tag="KPG09_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBG0966913.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            12083..12844
                     /locus_tag="KPG09_002307"
     CDS             12083..12844
                     /locus_tag="KPG09_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HBG0966914.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLEDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            12857..13486
                     /locus_tag="KPG09_002308"
     CDS             12857..13486
                     /locus_tag="KPG09_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG0966915.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            13528..14253
                     /locus_tag="KPG09_002309"
     CDS             13528..14253
                     /locus_tag="KPG09_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HBG0966916.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEILEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            14266..14538
                     /locus_tag="KPG09_002310"
     CDS             14266..14538
                     /locus_tag="KPG09_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HBG0966917.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            14531..15082
                     /locus_tag="KPG09_002311"
     CDS             14531..15082
                     /locus_tag="KPG09_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG0966918.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYVVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            15094..16185
                     /gene="eutH"
                     /locus_tag="KPG09_002312"
     CDS             15094..16185
                     /gene="eutH"
                     /locus_tag="KPG09_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBG0966919.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NSGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLRKENV"
     gene            16187..16660
                     /locus_tag="KPG09_002313"
     CDS             16187..16660
                     /locus_tag="KPG09_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HBG0966920.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(16780..16881)
                     /locus_tag="KPG09_002314"
                     /pseudo
     CDS             complement(16780..16881)
                     /locus_tag="KPG09_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            17176..17697
                     /locus_tag="KPG09_002315"
     CDS             17176..17697
                     /locus_tag="KPG09_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0966921.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
BASE COUNT         7352 a         1977 c         3270 g         5210 t
ORIGIN      
        1 ttttataata tatgactatt tattttattt ctttactatt caagttctaa atcaataata
       61 aattttgata tttaattgta tcaggacaaa tctctaattt catatattat taatataaaa
      121 tcataggtta gtgtgaaaaa gttgaaattt aaatatttta aaagataatt ttttggaact
      181 aataggtata acaaaaattt atagggggat tgcatatgga aatcaatgtt atagaaattt
      241 tccctaaaga taaagctaaa cttaataaaa tagaaatgga taaagctagt tggtttgtaa
      301 atataataag taaaaaatat cctaaagaag ctttaaatga agcatttagt attttagaaa
      361 aagaattaaa tataagtaaa gctaatacat aagctttgat tattgatttt atatactatt
      421 ggttttatac gctatgactt gacattatat taatcaaata gtatattttt aaatatagca
      481 caatgaagtg ttaaaagagc aatggagctc catagataaa gtctatggag cttttttgcg
      541 tatttaactt aaggaggata gagctttgaa taactttgag attaaaacta gaataaaatt
      601 tggagaaggt tcactaaaag ttttgcaaga attaaaaaac aaaaatgtat taataataac
      661 agaccctttt atggtgaaat caaaaacaat agataaaata acatctaatt taataaactc
      721 aacatatcaa gtttttgaag aaatagtacc agacccacca atagaagtag tagtagcagg
      781 aatagaagca tttaaatcta taaatccaga cgtaataata gcattaggtg gtggttctgc
      841 aatagatgca gcaaaatcta taatggactt ttctaaaaaa atattaaaaa ttaacgaagt
      901 agagtttata gcaataccaa caacaagtgg aacaggttca gaagtaacat catttgctgt
      961 aataacagat agtcaaaaaa aatgtaagta tccattagtg agtgatgatt tacttccaga
     1021 tatggcaata ctagacccag agcttgtaaa aactgtacca aattttataa cagcagatac
     1081 agggatggat gtattgacac atgctataga agcttacgta tctacaaatt ctaatgactt
     1141 ttcagatgca ctagctgaaa aatctataaa gttagtattt aaatacttaa taaaagcata
     1201 taaagatgga aatgacatag aagctcgaga aaaaatgcac aatgcatctt gtttagcagg
     1261 tatggcattt aaccaagcat cacttggcat aaatcattct attgcacatg tattaggtgg
     1321 aaaattccat ataccacatg gaagaacaaa ttctatatta cttcctcatg taatagaata
     1381 taattcatca atagtaggat atactaacac aacttactct gatacagcta aaaaatatgc
     1441 acaaatagca gaattagtag gacttgggag tgctaatata agaatatctg ttagaaattt
     1501 agtaaatgaa ataaagaaac tacaaaaaga aatgaatatg ccaactaaat tgacacaatg
     1561 caaattaagt ttagaagata taactaatga agaaagagaa atagcaaaac ttgctataaa
     1621 agatacttgt acattaacta atcctaaggt accaactgaa gatgatataa taaacatatt
     1681 aaataacatt aaataactac attttattac atgtaaatat aatattatac aaatagggaa
     1741 aatgttttta aaaatataaa ttatatattg acaaatataa aacataaaac tataatgaaa
     1801 gtatcacaaa ggcgtgatac tttagtagaa agcaatggag cttcttagga atattaatat
     1861 tattcttatg gagctccttt tattttaagc aaatagcttt ttaataaact atattcaaag
     1921 aaagcaggga ataaatttga ctgaagaatc taagcaaaga gttattcaag aatatgttcc
     1981 tggaaaacag gttacattag cacatatcat agctaaccct aatgaagaca tatataagaa
     2041 gttaggatta gtacttgata aaagagatgc aataggaata cttacaataa caccaagtga
     2101 agcgtcaata atagcagcag atgttgctac aaaggcttca aatgtatctt taggttttat
     2161 agatagattt agtggttcag tagtaatatc aggtgatgta agttctgtag aatcagcgtt
     2221 aaatgatgtt ttagaagtct taggaaatat gttaaacttc tcatctacaa aaatcacaag
     2281 gacataataa tacaatgaag aatataatat ttatgggaaa gacaggtagt ggtaaaacta
     2341 ccttatgtca aaaactagac cagttagaat taaagtataa aaaaactcaa tcagtggaac
     2401 tttataataa tgcaatagat actcctggtg agtatatgga aaatagaaac ctatataatg
     2461 ccttaataac aacatcagca gatgctaaaa ttatagcaat agtgtatgat gccacacaag
     2521 aagaaaatta tatagcacca ggttttgcca gtatgttttg caaagacgta ataggaatta
     2581 taactaaaat aaataaagct actgaaaacg aagtcaatat agcttatgaa agactatcac
     2641 tagcaggagc aagtcaaatc tttaaaatag acacagttga tgatattgga cttgaaaaat
     2701 tatttaaata tctagaaaaa tctaaaaata tagatgaagt ttaaggggaa atattttttt
     2761 attcaaaaat attattaaag tatgataaaa taaaaaaatg tataaactat tttaagaaga
     2821 tttaatcttt aagcgaggta ggaacatggg gaaaaaaata ctattggttg atgatgaacc
     2881 actgttaagg atggatacaa aagatatatt agaaagtcat ggctatgaaa taataggcga
     2941 agcaagtgat ggatttgaag ctgttgaaat gtgtaaaaaa cacaaacctg atttagttat
     3001 aatggatata gagatgccaa tacttgatgg tataaaggca ggcaaaataa tatgtaaaga
     3061 aaatctagct ggtggagtac ttttattaac ttcatttgaa gataaagaat acgtagaaaa
     3121 ggctaaaagt ataggtgctt atggatattt agtaaaaaca gcaagtgaaa aatcacttat
     3181 cccaaccata gaaatgtgtt taaataaggt aaatgaattt gaaaaaatga aaaaagattt
     3241 agaaaaagta aattctaaat tgaatgaaag aaaattagta gaaagagcaa aaggtatact
     3301 tataagagag tttaagattt ctgaggatga agcatacact agaataagaa aattaagtat
     3361 ggataaaaga actacaatga tggaaattgc caaaatgatt ataataggat acgatgacta
     3421 atacgataaa aaaaatatca actgcacaaa catacttaaa taataacgaa attgaaaaaa
     3481 ttatagaagt atcaaaatcc ttagatttaa tggcttcttt ttatgaggct gatgttttta
     3541 tagatgtact aagtaaattt gatgatgaag caattgttgt ggcacatggt agacctaaag
     3601 aaaagtcaat gtactgtgaa aatgttatag gtaaaaaggc tttaattgaa aatgaaccag
     3661 gagttataaa gtgcttaaaa acaggagaaa gagtaagaga tattaaagct ttaacacagg
     3721 aatataaact ggtcaaacaa aatattcagc caataacact agatgacaaa gttttagcag
     3781 tactaatagt tgaaaaagat ataagtagtg aagttaagaa tgagtttaat gctagtgaag
     3841 acgaagaaaa taaaaattta catcagttta taaaacttat cggaaataat gactttttag
     3901 ttgataattt aaattcagcc atattaatat ttgataaaaa tggaaaatta aaaattaaaa
     3961 atcgtattgc aactgagata tacaaaaatc ttgggtttgg atatgatata caagatatgc
     4021 attacaatga tttagatata gaaaccaaca cctttgaaaa tatattaatg gatgaaaatt
     4081 atgatgagac aaatgaagtt tcaataggta atatgttttt taaaataaag accatataca
     4141 tcaaagatga tgaatttaag gtttgcaaaa tagtacaaga tataagtgat ttaaagagaa
     4201 aagaagaaga attagtttta aaaacagtgg ctataagaga agctcatcat agagtaaaaa
     4261 ataatcttca tacagtaata tctataataa aaaaacaaag tagattatct caaaatgatg
     4321 aagtaaagca gtgtttagat aacataataa acagagtatt tgcaatactt tcaactcatt
     4381 atttactatc taaagaagtt gataataata tatctatacg agaaggtatc aatttattag
     4441 tgtcaaatat tcaaggtggc tatttattta gtaaggatat agatatacgt ataactggtg
     4501 aagattttaa aattagtgga gaaaaagcaa cagcaatact tttggttata aatgaaatac
     4561 tacaaaattg ttatgaccat gcattttcgg atagagaaca tggtactata caaatattaa
     4621 taaataaaga taacgactat agaaatattg ctattataga tgatggaatt ggatttgata
     4681 gtaataatat aaataaaaat agcttaggga catatataat agatagttat ataacacaga
     4741 tgttaaaagg aaatatagag agaaaaagta atgagaatgg tacagaagta ttaattacta
     4801 tacctacaca aattaatatt taaataataa ctcatctaca aagtagtagt tttaattgag
     4861 caaagaggct taaggttatt aatagattaa gttaattgaa tgtatttata aaaattataa
     4921 gtatatattc aatttaacag gtatttatta taactttaag cctctttgct tgtttaagac
     4981 atacttaaat tttaggaggt gagaaaatga gagaagagtt attaagtgtt ggtattgaca
     5041 ttggaacaag tactactcag ttaatttttt ctaaattaat agtagaaaat actgaatcaa
     5101 atttttcggt acctagaata agtatagtag acaaagaaat tatttataaa agcaaagttt
     5161 atttcacccc tcttatttca aataatgaaa taaatggtca acaaataaaa gatatagttg
     5221 aaaatgaata taaaaaagca aatatatcta aagaggatat acatacagga gcagtaataa
     5281 taactggtga aacagctaga aaagaaaatg caaatgatgt acttcatact ttgagtggat
     5341 ttgctgggga cttcgttgtt gcaacagcag gacctgattt agaaagtata atatcaggaa
     5401 aaggagctgg agctcatatt tattcaaaag aacattcaac tacaattgta aatatagaca
     5461 taggtggggg aactagtaac atagcctcat ttaaaagggg agaagtaacc gatactgggt
     5521 gtttggatat aggtggaaga cttgtgaaga tagataaaat aagtaaaaaa ataacttata
     5581 tatcacctaa aatagataaa atcataaaag ataataattt aaatcttcaa gtaggcacaa
     5641 tggctactgt agaaaataca aagccaatag tggatataat ggttgactta ttaatggaaa
     5701 gcataggatt aaagaaacag acacctttgt ttgattttat aataaccaat aaatcaataa
     5761 gtatagatga aaatattaaa aatgtttcat tctctggtgg agtagctgat tatatttact
     5821 atgatggaga aataaaagat tattttgaat atggtgactt gggaatttta cttgggcaaa
     5881 caataaaaaa ttgtgaatac cttaaaaaag taaatgtagt taaatctatg gaaacaataa
     5941 gagccacagt ggttggggca ggttctcata ctactgaagt aagtggaagt acaataactt
     6001 acactagcga aaagtttcca cttaagaact tgccagtttt aaaattatca gttgatgaag
     6061 aaacaggtac ttcaaaacaa atcagtgaag ctataaaaaa taaacttaat tggtttaaac
     6121 tagaaaatga tttgcagaaa gttgctatag ccatgatagg aaagaaaaat ccaacctttt
     6181 tagatataca agagtatgct aaagggtttg tagatggaat gcaacaaatc atagaaaatg
     6241 atttaccttt tatagtaatt gttgaaaatg atatggcaaa agtgttaggt caagcaatat
     6301 atgcattatt aaattatgaa aaagaaattg tttgtataga cagtgtcaag gttgaaaatg
     6361 gagactatat cgacattgga aaacctatag ttgaaggtaa agttttacct gtggtaataa
     6421 aaacattagt ttttaattaa tagaaggagg taagtaaatg attttaaata caaaattatt
     6481 tggacgtact tatcagttta aaggcttata tgatgtaatg gctaaagcta atgaagaaaa
     6541 atcaggagat aaattagcag gtcttgcagc agaatcagct gaagaaagaa tagcagcaaa
     6601 agttgtttta tcacacataa aattggagga tataagaaat aatcctgcag tggcatatga
     6661 agaagatgaa gtaacaagaa taatacaaga tgctgtaaat gaaaaagttt atgaagaaat
     6721 aaaaaattgg acagtatctg aacttagaga atggctattg gattcaaata ctacaagtct
     6781 tgatataaga agaatatcaa gaggattaac ttctgaaatg atagcagcag ttgcaaaatt
     6841 aatgtcaaat atggacttag tttatgcagc taaaaagata aaggttactg ctcactgtaa
     6901 tacaactata ggagaacaag gaacattatc agcaagactt caacctaatc atccaacaga
     6961 cgacccagat ggaatacttg catcacttct tgaaggttta acttttggta taggagatgc
     7021 agtacttgga ttaaatccag ttgatgactc tgttgagagc gttactaaag tattaaaaag
     7081 atttgaagaa gtaaaacaaa aatatcagat accaactcaa acatgtgtat tggcacacgt
     7141 tactacacaa atggaagcaa taagaaatgg tgctcctgca gatttaatat tccaatctat
     7201 agcaggttct gaaaaaggaa atgaagcatt tggatttaat ggcgacacta tagaagaagc
     7261 tagacagtta gctttaaaac acggtacagc tacaggacct aacgttatgt attttgaaac
     7321 tggacaaggt tcagaattat cttcagaagc acatcatgga atagaccaag ttactatgga
     7381 agctagatgc tatggatttg ctaaaaagta tgaaccattc ttagtaaata cagttgttgg
     7441 gtttatagga cctgagtact tatatgatgc tagagaagtt gtaagagcag gacttgaaga
     7501 ccactttatg ggtaaactac atggcttacc aatgggtgtt gatgtttgtt acactaacca
     7561 tatgaaggct gaccaaaatg atatggaaaa cttagccata ctattaacaa cagcaggatg
     7621 tacgtatttt atgggaatac ctcatggaga tgatgttatg cttaactatc aaacaacagg
     7681 ataccatgaa acagcatcac ttagagaaat gtttggacta acagcaataa aagaatttga
     7741 tgcttggatg gaaaagatgg gattctataa agatggtaag ttaacagagt tggcgggaga
     7801 cgcatctgta ttattaggat aggaggtatt taatatgaat gagaaggatt taaaagcatt
     7861 ggtagaacaa ttagttggac aaatggttgg agaattagat actaatgttg tatctgagac
     7921 tgttaaaaag acaactgaag ttgtagtaga caacaatgct tttatagatg atataacaga
     7981 agttgatata agaaagcaat tactagtaaa aaatcctaag gatgcagaag cttatttaga
     8041 tatgaaagca aaaacacctg caagattagg tataggaaga gctggtacta gatataaaac
     8101 agaaacagtt ttaagattta gagcagacca tgctgctgca caagatgctg tattctctta
     8161 tgtagatgaa gaatttataa aagaaaataa tatgtttgca gtggaaactt tatgtaaaga
     8221 taaagatgaa tacttaacaa gaccagattt gggaagaaaa ttttctcaag aaactataaa
     8281 taatataaaa tctaaatttg gtacaaatca aaaggtttta atattagttg gagatgggct
     8341 tagctcagct gcaatagagg caaatttaaa agactgtgtg ccagctataa aacagggtct
     8401 aaaaatgtat ggtatagatt caagtgaaat tttatttgtt aaacattgta gagttggtgc
     8461 tatggaccac ttaggtgaag aattaggttg tgaagttata tgtatgttgg ttggagaaag
     8521 accaggatta gttactgctg aatcaatgtc agcatatata gcttataagc catatatagg
     8581 aatggcagaa gcaaaaagaa cagttatatc aaatattcac aaaggtggaa caactgcagt
     8641 tgaagcaggt gctcatatag ctgaacttat aaaaactatg ttagataaaa aagcttcagg
     8701 aatagacctt aaataaagtc gagggagtgt agaatatgaa aaatgatgta attcgtccta
     8761 atatactagg aattaaaata atttcgaata taagtccaga aatggcacaa aaactagaat
     8821 taaaatcaca tcacaaaagt ttaggtttaa taactgcaga ctgtgatgat gttacgtata
     8881 cagctcttga cgaagcaact aaagcttcag aggttgatgt tgtatatgca aaatcaatgt
     8941 atgcaggtgc tgcaaatgct tcaacaaaac ttgctggtga agtattagga ataatagcag
     9001 gcccaagccc agcagaagta agaagtggtc taaatgcagt agttgatttt ctagagtatg
     9061 gagcaacttt tataagtgca aatgatgatg attcaatagc atactatgct cactgcgtat
     9121 caagaacagg tacatacctt tcagagatag caggaataag agaaggtgaa gcattagctt
     9181 atttagtagc accaccactt gaagcaatgt atgcactaga tgcagcaatg aaagctgcag
     9241 atgttaagat gtgtgaatta tttgcacctc caactgaaac aaactttggt ggagcattac
     9301 ttacaggttc tcaatcggct tgtaaagcag catgtgatgc atttgcagaa gcagtaaaat
     9361 cagttgcaga caatccaaca ggattttaag gatagttaga tggatgctat aggcttaata
     9421 gaagtaatag gatatgttgc tgcaatagaa gcttctgatg cttgtttaaa atctgcaaat
     9481 gttaatattg taagaattga taaggttggg gcaggtattg ttactttaac aatatgtgga
     9541 gatgtcggtg ctgtaaaagc tgctttagaa gcaggtgaaa tagcagcttc cagagttggt
     9601 acattaagaa catcacatat tataccgaga atccataatg aagtaacaaa tgctttattc
     9661 aaaactaaag aacctaaagt ctgtgaagtt tcagaagata aagaaaaaaa ctttgagatt
     9721 acattggaaa taactgataa tgtatcgaaa aatttatcag aaaaagaaaa tgtaatttta
     9781 gatgtcaatg aaaacagtgg agatactgaa aatttatata gtgatataga taaaacaaac
     9841 aaaaaaattg aaacagttga tgatagttta acagaagcta atgaggaaaa aatagaaaat
     9901 actaatgata aagaagtatt ggaacaagtt aaaatagaaa attccgattt tgatagagat
     9961 gacctatcaa agaacagtgt aaaaaaactt aaagctatgg ctaagaaatt agattcttca
    10021 ataacttata aagatttaaa tgttcttaaa aaagaagaat taataaactt agttaaaaag
    10081 cttagcagaa gggatagata gttatgacac ttttagataa agatttagta tctatacaag
    10141 aagtcagaac tcttgtaaga agatctaaag tggctcaaga gaaactttct aaaatgagtc
    10201 aagagcaaat agataaaata gtaaaagctg ttgtagatgc agcatttaat aatgctacta
    10261 aattagctaa aatggcaaat gaagaaactg gatttggaaa atgggaagat aaagtaatta
    10321 aaaatacttt tgcatctatg ggagtttatg aaactttaaa gaacacaaaa actattggaa
    10381 taattggtga agataaagaa gccaatacga tagatatagc tacaccagtt ggagttgtgg
    10441 caggtcttat accttcaaca aacccaacat caacagtaat atacaaaact ataatatctc
    10501 taaaagcagg aaatactata gttttctctc cacatccaaa tgcaaaaaac tgcatacttg
    10561 aaacagttaa tataataagt gaggctgcta taaaggctgg agctccagag gattcaatat
    10621 tatgtataac taatccaagt atagttggaa ccaatgaatt gatgaaacat aaagatacaa
    10681 acttaatact agcaactgga ggagaagcaa tggttagagc tgcttactct tcaggaacac
    10741 cagctattgg agttggacca ggaaatggac cagcatttat agataaatca gcaaatgtta
    10801 aattagcaat caaaagaata atagattcta aaacttttga taatggtgtt atatgtgcat
    10861 cagagcaatc tatagtaata gaaaaatcta tggaagaaac tgttttaaaa gagcttaaaa
    10921 atcaaggtgc atatgtttta aatgaagagg aaagtgaaag attagcaaaa ttcatattaa
    10981 gagctaatgg tactatgaat ccacaaatag ttggtaaaag tgttgaggta atagcaaatt
    11041 tagcagactt aacaaatata cctaaaaata caaaagttct agttgcaaga gaaaataaag
    11101 ttggtcacaa tgtagcctat tcaagagaaa aacttacacc aatactagcg ttttatgttg
    11161 aagaaaatgt agaagctatt atgaacagat gtagagaaat attgttaaat gaaggtgcag
    11221 gtcatacatt ctcaatgcat gtaaatgatg aagaattggt aaaaagattt gcccttaata
    11281 tgccagtatc aagaatactt gtgaatactc cttcagcact tggaggaata ggtgcatcaa
    11341 caaaccttgt accagcacta acacttggtt gtggtgcaat aggtggaagt tcaacttcaa
    11401 acaatatagg accacttgat ttaataaatg ttaaaagagt agcttatgga atcaaagaaa
    11461 tagaagaact tggaagagat aaatctcaag ttgaaaaatc aatgtttgac tcaatggata
    11521 aagaagaatt aataaatctt cttgttcaaa aactagtcaa agaactagca taactaaaat
    11581 aaacaatgaa acttatggct atcgctataa atttataaaa atataaacta taaaaaacta
    11641 taaaattaaa tttttggagg ataagattat ggcaagtgca aacgcattag gaatgataga
    11701 aactaaagga ttagtaggag caatagaagc agcagatgca atggttaaag ctgcaaatgt
    11761 acaattagta ggaaaagagc aagtaggtgg aggattagtt actgtaatgg taagaggaga
    11821 tgttggagct gttaaagctg ctacagatgc aggagctgca gctgccgaga gagttggaga
    11881 actaatatca gttcatgtta taccaagacc acactttgaa gtagatgcta tattaccaaa
    11941 ggtatcagct gagtaatata agaataattt taaaatttaa attttgattg aaaaagaggc
    12001 tatctcaaaa atgagtctta ctaataaact caatgtattt tgaggtagtc ttttttatac
    12061 atctaatagg agatgtgata aaatgggtgt tttaacagaa aacgaagtaa aaagaaaaat
    12121 tggaaaaaac aaaaataata taactgaatt ttatatagaa aaaggtcaaa taataactcc
    12181 ttctgcaaaa tcttatttag ctgagagaag tattgagctt aaatatgttg ataaactttc
    12241 agatgtaaac aatgaaccta aagaagttgt aaaagaagta gttaaagaga aaaaatataa
    12301 atatactact gtatttggcg ctaaattgga tgaaaaacca gaacatatga ctcaattaaa
    12361 tggaaatctt ttagttttta aagaccataa aagaataata ttgagaggaa aaatagattc
    12421 tcttgaatca gaaatactaa aggtacagat actttgtcag aaactagata tgccaaagct
    12481 tgttaaggaa ttgcaagaag tattagactt tgtaagaaat ataatgagat gtgaagtttt
    12541 agatgaaaag ctagaagact ttaaactttt aggattaact cctcaagaac ttagagaaca
    12601 atctcactat cctaaaaaat actttggtat aggacatgaa ttcccaagtt atgaaatggg
    12661 agaagtagta gtagctataa atagtataag aagtaataca cgagaggttg aacttgcagc
    12721 ctatgaagca tttaagggtg agtatggaaa tgttcacaga gaagatttaa taaaggcact
    12781 taatagatta tcgtcagtat tttggataat gatatataag gttagaacta ataagtataa
    12841 gtagttggag gacataatgg agaatctatt aaatgaaata gtggaagaag taataactag
    12901 agttaaaaaa gaagcattta tagaagttga agcatcagga agacacgttc acttaagtag
    12961 agaagatgtc gataaattat ttggagaagg atatactctt acaaaactaa aagacctatc
    13021 tcaaccaggt caatatgcat gtaaggaaag agttacaata actggtccta aaggaagtat
    13081 aaaaaatgta gttgtacttg gaccatgtag aaatgaaact caggtagaaa tatctttaac
    13141 agatggttca actcttggac taaaagctcc tataaaacaa agtggagact tagaaggtac
    13201 tttaagtatt aaaatatcaa cacaatatgg tgaagttgaa cttgataagg ggttaatggt
    13261 tgctaaaaga catatacaca tgactcctaa agatgctgaa aaattcaatg tatgtgataa
    13321 agagatagtt gaagccaagg tgatgggtca aagaccttta acatttgatg atgtagtaat
    13381 aagagtgagt gacagtttta aaacttatat gcatatagac tatgatgaag ccaatgcatg
    13441 tggttattca aaaggaactg ttgctaaaat aataaagaaa gcataaattt aacctactat
    13501 ataaattacc tctagggagg atttaatatg aattatgata accttgtaga tttaataacg
    13561 gaagaaatct ataaaaaaat aaatagtaat gaaataaaaa tatctaataa acctaaagct
    13621 gtgattgtat ttgaacaaga taacaataaa tttaacttat taaaagatga atttgaaata
    13681 ctagaatttg ataaaagtat aagggaatgt gaaatagtga tagtttcaag actttgtatg
    13741 agggggctat cgaatatagc acttggaaat tcaacaagtg atgaagaaag atttatacta
    13801 aaaatgatta tgaaaggcaa aaaagtatat gtcctagatg aaggaattga atacaaaaaa
    13861 tataaggata ctgcaccaaa agcattatat aagaaattta tgtcatttga agatgaaata
    13921 tgtaaatttg gtgtagaaat tataaaagac ttaaatgtta taactaaaaa taagttaaat
    13981 ataaagaaag aactagaaag tactagaggt tctaaaaaag aagatacaaa agttttagat
    14041 aaagatgttt taaatttaac cagtgtagaa agtgtaaaaa tagataatga atttagtttg
    14101 gatttaagga ataaaaaact tatatcggaa gctgacttaa gaaaaccaac tataaatgga
    14161 gttaaaaaca tattagttaa taaaaaaagt ataataacac cattagctgt agattttatg
    14221 agaatacacc atctcaaact aaagaaactg taaggaggta attttatgga tataggaaaa
    14281 gttgtaggta atgtttgggc tacaagaaaa gatgaaaaat tatgtggcca aaaactttta
    14341 gttgtaaaaa ttttaaaaac taaagacaca tacaaggaag gcttcatggt tgcagccgat
    14401 aatgcaggag caggaaatgg agacttagtt cttatatcca aaggttcctc agctagagag
    14461 tcgattgaaa aatctcatgt tccagtagat gcaactatag ttggagtagt cgattcgttt
    14521 gaggtgtttg atgaataaaa gtataggagc tatagagttt aaaagcattg ctaaaggtat
    14581 agaagtatcc aatgaaatga taaagaaatc ttcagttgat gtattgtact taaaaagtat
    14641 ttgcccaggt aaatttctaa taatagtagg tggagaaact tcttatataa atgaatgtgt
    14701 agattatgga ataaaacttg gagaaggata tattgtagat aacttcgtaa taaatgcaat
    14761 ttcacaagaa atattagatg gatttaaaaa taagtatcaa aagctagata gtatagtctc
    14821 tataggcgtt gttgaaagta gtaaagtatg cgctggaata aaaatgcttg ataagacttt
    14881 aaaatctggc gacttagtat tagtaaaact tcaattatct tttgctatag gtggcaaatt
    14941 agtatatgta gtagcaggag atttaagtag tttagaatat gcattaaaag aaagtgaaaa
    15001 tgtagtaaga gaaaaagaag taatatataa gactgtaata ccatcagtag acagtcaaat
    15061 aataaaaagt ctaataaaat aatgggggga attatgagta taaacgaaat tataatctat
    15121 gtaatggtag tcttcatggt acttggagct atagataaat gtataggtaa caaatttggt
    15181 cttggagaac aatttgaaga aggtataatg gcaatggggt cacttgcagt tgcaatggtt
    15241 ggtgttatat gtttagcacc agttcttgct gatgtattaa gaccaattgt agtaccagta
    15301 tttaatatac ttggagctga cccagctatg tttgctggaa gtttattagc taatgatatg
    15361 ggtggtgcgc cacttgcact tgaacttgcg aaagacccta attctgggtt atttggtgga
    15421 ttaatagtag gggctatgat gggacctaca atagtattta taataccagt tgcgcttgga
    15481 ataattgaaa aagaagacca aaaattctta gcaacaggaa tacttgcagg tataataaca
    15541 atacctattg gagcattcgt tggtggatta gttgcaggat tcgaggtaat gatggttctt
    15601 agaaacttag tgccaataat aatatttgca gtagttatag cacttggatt attaaaattc
    15661 gaaaatgcta tgataaaagg attcacatac tttggaaaag gtgtagtaat agttataact
    15721 ttaggacttg ctgctgccat agttgaagct ttaacaggaa tagttgtaat acctggaatg
    15781 aaaccaatca gtgaaggtat agaaatagtt ggagatatag caatagtttt agcaggagca
    15841 ttcccattag tgtttgttat aacaaaggta tttaataaac cattaatggc tttaggaaaa
    15901 gttcttggaa tgaatgatgt atcagcagca ggccttgttg caagtttagc taactgtata
    15961 ccaatgtttg gtatgatgaa agatatggac aatagaggaa aaataataaa tgtagcattc
    16021 tctgtatcag cagcattcgt atttggagac cacttaggat ttacggcagg atttaattct
    16081 aatatgataa caccaatgat agttgctaaa ttagttggtg gtataacagc agtattgtta
    16141 gctatggtta tcgcaaataa gacactaagg aaggagaatg tataagatgg atatatcaaa
    16201 tatagataaa aatttaattg aaacattagt aagacaaata atagaagaaa aaataagtgg
    16261 aactaaagac acagtagatt ttgttagaaa taaagacatt agtggaataa cttctataaa
    16321 gttaccaact gtaaaagtta gtgaatctga tagattagat acagggaatc caagtgatgt
    16381 agtttacaca aaagatttat ttacattaga ggaaagccca agattaggtt gtggtatgat
    16441 ggaaatgaaa gaaacaactt ttgattggac tctaaactat gatgaaatag attatgtaat
    16501 agatggaact ttagatataa taatagatgg aagaaaggtt tctgcttctt caggtgaatt
    16561 aatattcata ccaaaaggaa gtaagataca attttctgta cctgattatg ctagatttat
    16621 atatgtaaca tatccggcag attgggcatc tcaaaattaa taaaaatcaa gttaagttaa
    16681 taataatcaa ataaataatt taaaatacaa actcttaaat aaacacacat atttaaaaaa
    16741 gctatgaatt aaaattcata gcttttttta taaagaatgt tatattttag acgtattttt
    16801 tctatattta ctgggagaca tattatattt tttattaaat aatctaataa aatgctctac
    16861 atttggataa ccaatactat tttactgata atcttttcct tatcactaat cttacatttt
    16921 aaaatattat ttttactcgt aatattatca taagaatttg tttcttattt aaaactaatt
    16981 agaattataa agtttattgc catgttatta ataaaataaa tccagttaaa tcgagtgcta
    17041 gttaaagtaa taagaaactt ttacaatggt ttcctttatc gattgaaata aaagttaata
    17101 tggtataata tctaaagaga gaatcttatc aattagaata tgcatagaaa caagtggctt
    17161 aaaaggagac gttatatgga agagatagta aagaaggaac aagcaagtat gaatgatatt
    17221 tttttgtatc ttaaagctaa atcaaactca ttatataaat ttgtaatatt atataatgag
    17281 tttatcaata aacctaaaga ttatggaact ggggaactta taaacatggt tgaagtacat
    17341 atgctaacag cgattgaaga aaatccagga ataagtccaa ctcagttagc taaaatatgg
    17401 aacagaacta aaggtgcaat atctcaaact atatctaaat tggagaaaaa gggttatata
    17461 actaaagaaa agaaagaagg aaatgcaaaa actatattac tatatccaac cacatcagga
    17521 gtagagttaa gtaaggcaca taaactatat gacaccatag atgttacaag aacatttgaa
    17581 tacttaatca aggagtgtac attagaggag atagatagtt tttataaagt aattgatttg
    17641 tttattaatt tattagaaga atctccgcag gaaaataatg attctgaaaa agtgtagcta
    17701 agattgaagt tgaataaaga gaaatagata ttatagaagt agtacttaag tacacattat
    17761 ataaaaatat aataggtata ggattaaaaa gtttagtggt ttaaagttt
//
LOCUS       DAELSY010000078        17460 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.078, whole genome shotgun sequence.
ACCESSION   DAELSY010000078 DAELSY010000000
VERSION     DAELSY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.078"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            231..434
                     /locus_tag="KPG09_002316"
     CDS             231..434
                     /locus_tag="KPG09_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0966922.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
     gene            598..1752
                     /locus_tag="KPG09_002317"
     CDS             598..1752
                     /locus_tag="KPG09_002317"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBG0966923.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELVNITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFRVEQENIDVVVHPQSIIHSMVQYADSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            1765..2769
                     /gene="rseP"
                     /locus_tag="KPG09_002318"
     CDS             1765..2769
                     /gene="rseP"
                     /locus_tag="KPG09_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBG0966924.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSNKVVNVTPKNNNGKYEIGIQPQREKDFLASIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            2952..4007
                     /gene="ispG"
                     /locus_tag="KPG09_002319"
                     /gene_synonym="gcpE"
     CDS             2952..4007
                     /gene="ispG"
                     /locus_tag="KPG09_002319"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBG0966925.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSRERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKHGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            4355..5179
                     /locus_tag="KPG09_002320"
     CDS             4355..5179
                     /locus_tag="KPG09_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966926.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLASTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSIWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(5652..6775)
                     /locus_tag="KPG09_002321"
                     /pseudo
     CDS             complement(5652..6775)
                     /locus_tag="KPG09_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
     gene            6972..7196
                     /locus_tag="KPG09_002322"
     CDS             6972..7196
                     /locus_tag="KPG09_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966927.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            7249..7773
                     /locus_tag="KPG09_002323"
     CDS             7249..7773
                     /locus_tag="KPG09_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0966928.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(7878..8024)
                     /locus_tag="KPG09_002324"
     CDS             complement(7878..8024)
                     /locus_tag="KPG09_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966929.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(8163..9089)
                     /locus_tag="KPG09_002325"
     CDS             complement(8163..9089)
                     /locus_tag="KPG09_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG0966930.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            9280..11151
                     /locus_tag="KPG09_002326"
     CDS             9280..11151
                     /locus_tag="KPG09_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBG0966931.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNASCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(11243..12178)
                     /locus_tag="KPG09_002327"
     CDS             complement(11243..12178)
                     /locus_tag="KPG09_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBG0966932.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            12698..13210
                     /locus_tag="KPG09_002328"
     CDS             12698..13210
                     /locus_tag="KPG09_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBG0966933.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKEAKMAIICKKIYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            complement(13365..14426)
                     /locus_tag="KPG09_002329"
     CDS             complement(13365..14426)
                     /locus_tag="KPG09_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBG0966934.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(14550..15443)
                     /locus_tag="KPG09_002330"
     CDS             complement(14550..15443)
                     /locus_tag="KPG09_002330"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBG0966935.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIHSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQNYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            complement(15453..16934)
                     /locus_tag="KPG09_002331"
     CDS             complement(15453..16934)
                     /locus_tag="KPG09_002331"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBG0966936.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKKFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSEINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     misc_binding    complement(17042..17273)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         6662 a         2067 c         2714 g         6017 t
ORIGIN      
        1 aatagatata taatagtaaa tattttttat atagttatgt tttagtaaaa taatgagata
       61 aatatatata aagcttaatt tttgaactta tggttttgtt ttattttaac atatttatat
      121 ttaataaact ataattgaaa tctatttaga taaataatta tttatctaaa tagatgaaaa
      181 ttttccaaaa tagataaaat gtgatagaat atattttgcg aggtgataac atgatttcat
      241 acgctccatt atggaaattg ttaattgaca gaaaaataaa gaaaatggaa tttgtaaata
      301 tatcaggtat aagtatttct gttttaggca gactaggaaa tgataaatct gtttctatgg
      361 atacaatgga gaagatatgt ttagcactag actgtaaaat agaagatgtt gtggaaataa
      421 aaaagggcat ttaataatac tagaatattt tataatattc cacaagatac aaaaaataat
      481 agattaattt taatctgtgg aataatatat gaaatttcta tgaatagtaa aaataattgt
      541 tttagtagtg ttataatgtt ttgacagggt atatttatta ttagaaagga ttattatatg
      601 aaaaagatat caattttagg ttctacaggc tctataggta aacaaacttt ggatgtggtt
      661 agagaacata gagataagtt tgagatagtt gcaatatcag ctaacagtaa tattgaatta
      721 ttgctagaac aaatagtaga gtttaaacct aaatatgtaa cagtttttga ggaaaataag
      781 gcattgaaat taaaagaaat gttgcctaaa aatatagaaa tagaagtttt agcaggaatg
      841 gaaggattaa aaataatatc atcacttgat gaagtggatg tccttttaac tgctgtagta
      901 ggtatgatag gattagtccc aactctttgt gctataaaga agggaataga catagcatta
      961 gcgaataaag aaacgttggt aactgctggt gaacttgtaa tgaaagaagc agaaaaatac
     1021 aatgtaaata ttcttcctgt tgatagtgaa catagtgcta tatttcaatg tctaaatgga
     1081 gaaaataaaa aaaatataga aaagataata cttacagcat ctggtggtcc atttagagga
     1141 aagaaaaagg gagaacttgt aaatataact aaaaatgaag ctttgaagca tccaaattgg
     1201 agtatgggaa gaaaaataag tattgattct tcaacgctta tgaataaagg acttgaagtg
     1261 atagaagcta gatggttgtt tagggtagaa caagaaaata tagatgtagt agttcaccca
     1321 caaagcataa ttcattcaat ggtgcagtat gcagatagct caataattgc tcaattaggg
     1381 tgtccagata tgagactacc aatacagtat gctcttacat atccagatag aatggaaagt
     1441 agctttgaaa gaatgaattt ttcaaaattt agtactttaa cttttgaaga accagattta
     1501 gaaacttttc catgtttaaa attggcctat gagtgtttaa aaatgggagg aacttattct
     1561 tctgttttaa attcagcaaa tgaagtttta gtaagtgaat ttttagagga taaaattggt
     1621 ttttatgaca taccatatta catagagaag actttagaag ttcatagtag cataagcgaa
     1681 ccaacattag aagaaatctt agaaacagat agatggagta gagcttatgt tgcaaatctg
     1741 ataaaaaaat aggaaggtga atttatgact ataatagccg cattaatact atttagtata
     1801 attgtattaa tacatgagtt aggacatttt atatttgcaa aaagaagtgg tatcaaagta
     1861 aatgagtttt ctataggtat ggggcctaag atatatagtg taaaaaaaga tacagagtat
     1921 tctataagag cacttccaat tggtggatat gtcagcatgg aaggtgaaga tgaagaacaa
     1981 ataagtccaa attcttttgg aaacaagtct atattacaaa gatttagtac tattgtagca
     2041 ggaccaatat tcaatataat attagcagca atacttttag ttccagtatt tttgtatata
     2101 ggttctccaa cgactaaatt aggaaagata atgccagata ctcctgcaca ggctgtaggg
     2161 cttcaagtag gagataagat aaataaaatt aatggtaatt ctgttaagac ttgggatgaa
     2221 gttgcaaata taataaacac ttcttctggt ggagaattaa aacttagtat aactagagat
     2281 ggaagtaata aagttgtaaa tgtaacacct aaaaataaca atggaaagta tgaaataggg
     2341 attcaaccac aaagagaaaa agactttttg gcgtcaatag taaatgcatg taaaactact
     2401 gtagatatga caaaacagat gttaacattt ctaggtcaaa tgattacagg acgtgtacca
     2461 ggagggattg gcaatgctgt agcaggacca gtaggtgtta ttggtatggt atcagatgct
     2521 gctcgtacag gcatcataaa tgtagtgtat ttagcagctg tcataagctt aaatttaggt
     2581 atagtaaatc tattaccaat accagcactt gatggatgga gaatacttat gctattacta
     2641 gaagctgtta gaggtggtaa aaaactcgac ccaaacaaag aaggcatgat aaatgttgtt
     2701 ggttttggag ctttaatgtt atttatgctt tttatcacat ataaagatat attaagatta
     2761 tttcaatgat ttagtttttt agagatttta agaaaatagg ctatgtagtt ttaaataaga
     2821 ataaattaat ttttatgtat gaaatttatt cttatttaac tagatagctt tttaatttat
     2881 aatatacatg taggttataa tattttacta tttaaagaga ttcaatataa aataatatat
     2941 agaggtggaa tatgagttac gagagaagaa agacgagaga agtaagtgta ggaactgtaa
     3001 aaataggagg agaaaatcct ataagtatac aatctatgac aaatactgat acaagagatg
     3061 cagatgctac aatagctcaa ataaagagac tagaggaagc tggttgtgat atagttagag
     3121 ttgctgttcc tgatattaaa gctgctaaga atatagcaaa gataaagtct agtgtaaata
     3181 taccaattat tgcagatata catttcgatt ataagctagc ccttgaagct atagaacaag
     3241 gtgtggatgg aataagaata aatcctggta atattggaag tagagagaga gttaagatgg
     3301 tagttgagaa atgtaaggaa agaaatttaa aaattagaat tggagtcaat ggaggttctt
     3361 tagagaaaga acttttaaaa aagcatggct ctgcaactgc tgaagcatta gttgaaagtg
     3421 caatgggaca tattaagatt cttgaagact tagattttca taatatagtt atatctctaa
     3481 aatcatccga tatttataaa actgttgatg catatgaatt aatatcaaaa aaagttgatt
     3541 atcctcttca tattggaatt acagaatctg gaagtgttca caaaggaaca ataaagtctt
     3601 ctataggggt aggagcactt cttcttaaag gaattggaga tactgttaga atatctttaa
     3661 caggcgaccc tgttgaagag gttattgttg gtaaacagat tttaagaagt ttgggacttt
     3721 taaatgataa gattaaagtt atatcatgcc ctacatgtgg tagatgtaat atagacttaa
     3781 ttagtgttgt aaatgaggtt gaagaaaaga ttggtagtat ggaaaaaaat atcactgttg
     3841 caattatggg ctgtgctgta aatggacctg gtgaagccag agaagcagat ataggtattg
     3901 ctgggggtaa aggtgaaggt cttttattta aaaagggtga aatagttaga atgattgatg
     3961 gtaacaaatt agttgatgag ttgctagaag agattgaaaa attatagtat acaattcata
     4021 tatatattaa ttgactagat ttttaaatac ttttgtaata agtttttatt tacagtattt
     4081 aattatctag tcatttttta ttttataaac ttcattaaat gtaagaaact tatattctaa
     4141 tttgactata tatgtatgag ttaaatcata ttgtaaattt ataaaattaa aaaaagattt
     4201 aatatagtga aaatttccat caatagtatt gaaacttgat agtaactatg ataggatata
     4261 gttactagaa cagttggtaa attatgttaa catagatttt aatgttttgg taaattgttt
     4321 tattataagt taatagaaaa agaggtgaag aactgtgaga tataaaaaaa tatctagctt
     4381 acctatttta gttgttgctt tgctgattac tggaacgaca acaacatttt ctcttagtac
     4441 gaccaattca gaaaaaacaa atgtatactt agcaagtacg gtaagtactg tcacaaagaa
     4501 agtatccgat attcaaagta gtaaaggtct tgtttataat gagaatattc cattatttat
     4561 aaataatagc aaaatacagt atgatgatac tccatatcat tggcctagca atgttataag
     4621 cttaacaaat agcagtgaaa aggcaattat ggattatgag attacatgct tagcatatga
     4681 taaaaatgga aaaccacttg agctatattg ggatgctcaa aatgtggcag cagatggcga
     4741 agtaggaagt gttggattta gcccagctgg tgtagactat ggtattgtta caggcatatc
     4801 tcctgtgtct ccaaaatcat attcacatac ttatagaaaa atgcaacaat caccaccaca
     4861 ggatataata agtatgtttg aaaaacaaca aggcaaggca tgggtagaaa attggttgaa
     4921 agaatggaaa caaatggaga aagaatatgc aaagcaaaat gcaatagcac caggtaagaa
     4981 tcaaaatgat gcctttctat tgtttgataa atggaaacaa tcaactggag agcatggtgt
     5041 aaagtacatt atatcttgtg taaaacaagt aacatttaat gatggtagta tatggaaaaa
     5101 ttcagcatat gagaattggt tgaaaagttt tcaagggaag gaagtttcaa atagtgtatt
     5161 agaaaattac tataagtaat gtttaatgat agcaaaatga gtacgaattt aattaatgtt
     5221 aatttaatta aataaggact attttgttta aaaatatcag tgttacaagg aaatttttat
     5281 tgtgacaaaa tggtatatat tcaaaataaa aaagcatgtg tcaagttttg atacatgctt
     5341 ttttattttg aatatatgtt ttctataaat ttatgatttt ttagttatgt aattttatat
     5401 gtgaattttt agatttttat atagatagtg aaataatgtg gaagttttat gttttaaaaa
     5461 ttttagttaa aacatttatt aaaaaagata atagaaatgt tttaactaaa atagttagaa
     5521 ttgaaatgtt atacagatag tataagtact atcaaattat ataagattgc ttgaagttaa
     5581 ataatttatt taaatagaaa acagctgatt taatatcagc tgtatatgaa taaaattttg
     5641 aattgtagtg tttagttata tggatataat tcataataag ttagcaataa atcaaccatt
     5701 ttaccataac tctgagtacc ttcagtaacg ccattagctt ttaagtaaga attgttaaac
     5761 tcattagaaa tttcattaat tttaccttca tatttttgcc aaaattcact ttcatttttt
     5821 aaatctctac ggacagaatc tgaaatgcca gcagaaatat caacatatgc attataatca
     5881 accttactta aagcagaagc agtataattt aaagctaaaa tatatccaga atagttaaaa
     5941 tcaatatggg ggtgttttat agaagtaaga tacgctataa aattagcctc atcttcacta
     6001 gcaaatcccc tttgatgtgc tatttcatgt tcaactgtac aaggaatata taagtcaggt
     6061 attgctatat ttacatttgc ctcacctgta aaaggaaagt atataccagt tattcctgta
     6121 tagcacatta gatttgatga aataatatac ttaggcttag aataactacc actaacacta
     6181 ggtaatatat ccaagatatt atcgtatcct agttgagctc tatttatgac accattataa
     6241 tctgtattag attttacaat accattttta tcttgtaatg tgagttttct agtttcattt
     6301 gctttaatta caaggaattt atataattta gtaagctctt taacactata ttgttttgat
     6361 tggatagagg tattgttttt taaattatag ttatttataa gtgtggtttc tagtggaatc
     6421 ctattatagt ttattcccca aagcactaca aaaagaaagt agactattga tacaatggat
     6481 aatatattta aaatggaatt ttttaaaaat atgtttaatt ttttttctta ttaaaaattg
     6541 tagataaagt acaacataaa aataagaaga tagatataac tattatgtac attgtaatct
     6601 catagagaga aaatgggaaa attccagaaa ctttactcaa tatttgtaca attattttat
     6661 ctatgaattg ggagtaatat ttttcaacaa tatttggaac tttgctggct ataaaattta
     6721 aaagtaaagc aataggaaag agaattaaag aaaaatattt tgtttttttg ctcatatttc
     6781 acctcacata cattagttta tataaacata ttatatatca attgaagaaa gattgtaaaa
     6841 taagtataaa tatatctatt tataacttta tactaaataa atgataagtt ataatatatt
     6901 aatatgaata agcagtatat atacaactaa taataagaaa aatatcaatt atataaggag
     6961 tagataaaaa tatgataata aaacttacta aagacaattt taattttaga tttaaacaat
     7021 atgaaggtat gttttttcat gctgaagtag ttgaaggtat ggaatccatt agatataaaa
     7081 taactaagga taaagaaggt acagaattaa aagaatttgg aagtacacat ataaaagata
     7141 atatgatata tgtacctcag gtagaagcat acatagattt gaacagtata aaataaagat
     7201 gtaaaatttt aattaaaaat aaagattatg tttgaggggg aatccaatat gagtaagcct
     7261 atagatataa caataagacc tataagaaga gaagatgcac aagcttttaa tgaaattaga
     7321 agaacataca gtgttatgag aaatactttg gcattaatga gtgacagagt cgataaaact
     7381 gaagctatgt tagcttcttt gggagaaaat gattatatgt ttgtagctga aactaataat
     7441 aatggagaaa aagatgtaat aggttttgct gggttacatg taaattcttc tccacgattg
     7501 agacatagtg cagaaatagg aataagtgtt gcagagaatt atcagggaag aggagtaggt
     7561 aagaagctta tggaacaact tttagatata gcagataatt ggattatgtt aactagagta
     7621 ggattagaag taattgtaga taatgaaaag ggattaaatt tatataaaaa actaggtttt
     7681 gaaatagagg gaactaaaaa atatgctgta atcagagatg gaaaatttga agatgtttat
     7741 attatgggaa gatataataa aaatttaatt taaagaagct aatagagaag taaaatagag
     7801 tgtctttaat aaaaaattta cattaaaaat aatctatttt acttctctat tgtattaaaa
     7861 aacttatttt taaatgttta caaaaacatt ctatatacaa ataaaattat tagcaatatc
     7921 aacgctattt taaaagctaa actagcaaca gttttaatga tttttacaga aacacctata
     7981 gcaactatag caaatactat tttaaagagt aaatccatat ccatatttaa gcctcctatt
     8041 ctataataca tatagatatt attgacaaat atctaggaaa aaattccatc aatatactac
     8101 aataaaatgt ttatatttgt aatttattga atatgttatt atataataca taaaaaaata
     8161 aatcaagata agagtaaaat acttataaaa ggtatagata taactgaaaa tagtgttgta
     8221 aaaaacacac attgtgaagc tagtgttata ttggagtcat attgattagc cattatagca
     8281 gtatttgcag cagcaggcat tgaagaaatt actactggta tagctaaaat catcttatca
     8341 tttatccatc cttttagaat aaaatacaca atcacaggaa gtaccaataa tcttataaaa
     8401 gtgactatat ataatttttt gttgacaaaa caatcgagag ctgaagaatt agcaagtaag
     8461 cttccaatga taatcatgga tattggtgta gttatacttc ccaacatctc aagtggctcg
     8521 tttataaatt ggggtaacct aagtcctgtt acaaataaaa aaagtccaat aacaatagct
     8581 attgtagcag ggcttataag tgattttatt gaaaaacctc tacttgtact gcctttagat
     8641 aaaagataaa ttcctaaagt gaaagaaaat aagttaaaag gcaaattgaa tattgcagta
     8701 tagaatattg cttcatgacc taaaactgct tcaattacag gataacccat aaaagcaaca
     8761 tttgaaaaaa caactatata ctgatatatt cccaaatctt ttttatcatt acattttaaa
     8821 atgtatttca ataaaaatgc aattataatt gaaactatat acataatcaa agatgtaaat
     8881 aataacaata gtattttttg tatcatttta ctatcaaagt taatttgcat ggagctaatt
     8941 atcattgatg gtaaaaaaac tgtcattgtc aaagttgaaa gcttagatgt gcagtggtca
     9001 tctaataagt tgaattttct gacaaaatat ccaactaaaa taattataaa taaaactgca
     9061 acttggataa aaatattgct gaaattcata aaatgtcccc ccctatatat acattatata
     9121 ccaattacat aataaaatct aatgattata aaatttaaaa tttaatgtta caatatagtg
     9181 acaaaaggta aaaattcaat attatagcat taattataga ttataatgta taataatgtt
     9241 tgagagaata tattttataa aatgggtagg tagcgaataa tgaaaaatac aaaattttct
     9301 ataaaaaaaa taaaaaatat atctaatgac ccgaaagtcg tacatactaa gggtgccctc
     9361 cttgaaaaga tgggtaagat ggaaagcgca atagagttgt ataagagtgc tgcattggat
     9421 aattacgtaa aatctcaata tactttagga aatatatatg aaagtaaaaa acaatttaaa
     9481 gaagcagaaa agtggtattc aatggcatat aaaagtggaa gtgaagatgc tgcttttgat
     9541 ttaggtaata tgtactataa gttagatggc tatgaatatg ctatatattg gtatgaaaag
     9601 atagcaaatt taggttattt acaagcacaa aataatttag gtgtatgcca ttttaaaatg
     9661 aaagattttg taaggtgtga aaaatggtta aaaacagcag cagacaagaa tttaggtaaa
     9721 gcttgtttta atttaggggt attatatact tttttggaga aagatgatga agcatatgaa
     9781 tactataaaa aaggttctgt gcttttagat gatgatgcaa aatataattt agcaatatta
     9841 aatggaaaaa agaaagataa taaatcaaca gtaagtttat ataaacagtt gtacaagtca
     9901 ggtcatatga aaggttgctt taacttgggt atgattatgg aaataaatga taatttagaa
     9961 gatgcagaga agtattataa aaagagtgct gatagagatg atgtgaagtc tgagtatagg
    10021 cttgcatatg tatatgatag aaaagaagag ctgggagatg caatttatta ttatgagaaa
    10081 gcaatagaaa aaaatcatac actttctaag tatagacttg ctaatttata taatagagaa
    10141 aatgagatag agaaggcaaa aacttattat aaaatggctg ctgaagatga tataacagaa
    10201 gcaaaaaata atctggcagg tatatatttt gaagaaaaag attatgaaaa tgctattaaa
    10261 tattatgagg atgctatagc tgttggatgc aaaagttccc ttgaaaatct tggggattta
    10321 tattatcaga atcaagatat agaaaaagcg atttcgtatt attcgaggat tccaaataat
    10381 gcatcttgtc aaatcaaatt aggaaatatc tatgaagatt taaataatat tgaagaagcc
    10441 ataagttggt ataagaaagc atctgaaaat ggagatacga ggtctagtta tagattgggg
    10501 tgtatatatg agagcttagg aaatacaaaa aatgctagaa agtattttga aatggcaagt
    10561 agcaaaaatc atatgaatgc tcgtatacat ctaggtagaa tttattttag agaaggtaaa
    10621 ttggaagagt ctaaaatgat gtttgataca cctgccaatg aaaataatgt gtacgctcaa
    10681 catatggtag gattaattta tgatatgttc tataaagatt atgtaaattc taaattttgg
    10741 tatgaaaagg ctagagcaca aggttgtgtt gaatcaatat ataatttagg ccaaatttat
    10801 ttaaaattaa atgatgacgc agaagctgag aaatattata aagaaggtat taaatatgga
    10861 agtaagaagt gtgagtatat gcttgcaggt ctttattata aaaagagcct agatatgtac
    10921 atttctttag caaatgaaga gtacgataat tgtgaagaga tagtagaagg ctttcccaat
    10981 ttaaatatag actttgatga agtattaatt cctccattta agttgcaaga agaggtaata
    11041 gatgaagaag agtatgtacc aatttatata ctaaatataa aagaaagttt agattcaatg
    11101 ctagaagggc ttgaaactga aatgattata gatgaagaac atgatagtta aaaagtatta
    11161 taagattata tagatttaaa tatataaaag ccattaggat aaatgtaaat caattaattc
    11221 taatggcttt tttattaata ttttataata gcttatttct tgacatgaaa aatgtaatca
    11281 ctatacatat tatagaggta ataagaagta ttatccaaaa tccgtttggg ttattagaaa
    11341 atggcatgtt gatgacattc ataccaaaga atcctgatat tattgtaggt attgagaaaa
    11401 ttagtgtaac tactgttaaa atttgcataa cattattctg attattactt ataattgcac
    11461 tgaaagcatc cataattcta ctcaatatat ctccatatat tttagccata tcaagagcct
    11521 gtctattttc aactataaca tcttctaata ggtcttcatc atctggatac ttctttatac
    11581 cagcagttct aaccattttg tttaaaacaa gttcattaga ttttagtgag gtagagaagt
    11641 ataccaaaga tttttctaat tccaataatt gaaccaattc tttgtttttc attgatttgt
    11701 aaatttctcg ctctattatg attgtcattt tatttatttt tcttaagtaa tgcaagtaat
    11761 aagctgcatt tttgtgaaga atttgaagca aaaagcgagt ctttttaaac gtaaaaaaat
    11821 ctttaatatg accaactata aagtcattta aaatcttagt ctgtgcatca caaactgtta
    11881 caatagcttc ttcagttagt ataataccta agggtattgt agtataatga gaagaattag
    11941 agtcactttc gtctactggt atatctataa gtataagagt atgattgtct tcaacatcta
    12001 ttcttgagcg ttcttcttca tctagtgcag attctatgct ttctacatct atatttaata
    12061 aattagaaat ttcgttgatt tcactatgat taggttcaac aagatttatc caacagccat
    12121 cttcaaaaaa actaagtttc tctaattttg aatctatagt tttgtaatat cttatcatgg
    12181 caacacatcc tttattcagt tttatataaa tctcatcatt taatagtatc tattaattaa
    12241 ctaatttata taactgaata tatgtattag ttttgatttt agatattatt aagtgatgaa
    12301 aatgtaaaaa attctctccg caacggacta gtatccattg cccatcactc tccttattta
    12361 ttattttata tatagctatt tatgaaatgc gtattttaag cactctttaa tataatacct
    12421 tttccaggta tataaatcaa gttttgattt gattaaataa tatttttacc ataatttaca
    12481 cataaagtat ataagttata tttaatttga gtaaagaata tatatattct gagtgaaatt
    12541 attaaatata ttaaaaaata agagtgtata tatatattta cttatttgca ataatatatt
    12601 taaaaaagta ttttattttc aaaactagac ttcatttata atttatcacg aaaataaaag
    12661 gaatataaat ataagagaat gagaggttat tagagatatg agtgatttta aagaaatttt
    12721 ggcagaagag attaaaaata atccatttgg attgataggt aaagattgga cacttataac
    12781 agcagaaaat gaaggtaaag tgaatacaat gactgctagc tggggaggac ttggagttat
    12841 gtggggaaaa gatgttgctt ttgtagtaat aagaccacag cgttatacta aggaatttat
    12901 agataataca gataaatttt ctcttacatt ttttgatgaa gattttcgta aagagctttc
    12961 atattgtgga aaggtatctg gtagagaaga ggataagata tcacagattg gatttaatat
    13021 agaacactta aatgatacac cttattttaa ggaagctaaa atggctatta tatgcaaaaa
    13081 aatttatagt cagaaacttg aaccccaatg ttttattgct gaaggtatag atggaagatg
    13141 gtatccacaa aaagattatc atactttata tatagctgaa ataaccaatg tacttgtaaa
    13201 agaagattag ttataagggt taatactatc tctaattaaa ttaaaatttc aataaaagga
    13261 aattgacaca aaaaagctaa ggacataaat atgtttataa agattaataa ataacgtatt
    13321 aaaactcctt agcttgataa aattttaatt tagttttctt taaattataa ttcagaaatc
    13381 aattcattga taacatcttc aacagtactt aatttatcaa ttttataggc atcatttcca
    13441 caaaataaaa gtgaattttc aacatctcct ttaactgcat tgattagagc ttgagaaata
    13501 cagtaaggag tttcttttgg attacaagga atcaaacaat tatagcactt ctttatttct
    13561 ggacgattta ttttaacttc ttctataaat ttgtttctta tggcgcgtcc aggaagacct
    13621 acaggacttt taactatatg aacatcttct tcactagcat ttatgtatgc cattttaaag
    13681 ttctcatggg catcacattc gtaagtagca acaaatcttg taccaacttg tacaccactt
    13741 gcacccatat ctatatattt tttaacatca ctactagaag ttataccacc agcagcaact
    13801 actggtatgt ttctaccaaa tttttctcca taagtatttg caacctttaa aatttcaaca
    13861 aattccttat catagtctat agaatctatg ttatcaagtt cctcgttaga gtatccaagg
    13921 tgaccaccag cttttggacc ttcaacaact attaagtcag cagtagtttt ctcttttcta
    13981 tcccacattt ttaaaattac attagcagct tttgcagttg aaactatggg tgctattttt
    14041 acattacttc cctttactaa tgatggtagc ttattaggta atccagctcc agatattata
    14101 aggtctacac cagctttaac agcttccttt acgtatgttt catattcttt catagctacc
    14161 ataaaattaa taccaataat accaccattt gagatttctt tggccttaga aatatgtttt
    14221 tttatagctc ttaaatttgc atttatagta ttagtttcaa aatcttcctc atcatatcca
    14281 atttgaactc cagaaattac accaacacca ccaagcttag caactgcacc agctagtgat
    14341 gacctagata taccaacgcc cattccacct tgaattatag gaacttttgc aactaaatct
    14401 ctaattatta actcttttat gataatccct cctcaatgta aacatgttta aagaactaga
    14461 ttataatact taatattatt acctagtcct aattattata ctacaaatta taataaaaga
    14521 gtagttatat tttgtataga agacgataat tatattatat ttaaaacagc tccattagaa
    14581 tcgcaacata actcttttat ttcccaatca gattttagtt tttgtaggta atttttcata
    14641 ttatccacaa aactatcatc attttctttt attaaagaca ttatagtagg tccagctcca
    14701 cttaaaaata ctccaatact atttaattgt tcagattttt caacaatatc attgtagttc
    14761 tcaattagag ttcctctata attttgatgc aatttgtctt gacaagctac tttaagtagg
    14821 tcaaagtttt tgtttagtaa ggcagatatt agtaaagcaa ctctactaac attgaatacc
    14881 ccatcagaat atggtatttc ctttggtaaa acacctctag ctttttctgt tgataactta
    14941 aaattaggta tcattgcaca aaatttaagt tcttcaggaa tttttataat atcataatgt
    15001 atattctcat tatcagtaac agatacgatc atatttccta aaatagcagg agcaacatta
    15061 tctgggtgac cttctatttc tgaagctata tttaataatt gatttttatc taaattagca
    15121 cctgatagag catttgcagc tataactcca gctactatac aagaggcact actgccaaga
    15181 cctctacata taggtacttc actgtggatt tttattttga taccagcagg tattttttct
    15241 ggtttaactc tatcaaaaaa gtacttcata gatgtataaa ctaagttatt ttcgtttttg
    15301 taagcatctt cacaaccttc tatttcaaga ccactttcaa tttcttcaac ataaaattta
    15361 ttgtatatat ttaacgcaat tccaagacaa tcaaatcctg gacctatatt agcacttgtt
    15421 gcaggaacta taatctctaa cataataatc acctatacct ttaagaattt ttttatagct
    15481 agttttaatt catctttttc ataaatatct ttatgtagta tttctgcatt tttaagattt
    15541 ttaataggtt ttggaatatc tattttagaa atacttgata atctatcaat tatagcaaag
    15601 tcatcaaggt ctttaaaatc acaatctata gactttagaa catcttcaga aaacttgaat
    15661 ggacttgcag tacttacaat aacagttttt gtgttatcag aagtttcttt tttatatttt
    15721 tcataacaat tgtatgcaac agcagtatga gtatctatca aataattata atttttaaac
    15781 acattgttta tcgtgctttt aacttcttct tcatttgaat attctccata gaatgaggct
    15841 aaattttttt ccatatcatc acttattttg tagactcctt tatcactaag gtctgataac
    15901 agcttattta caatatcagt atttctattt gatatttcaa acagtaatcg ttctaaatta
    15961 cttgaaatta atatatccat agaaggagaa gtagttaatt tcaatgttct atttttatca
    16021 tatacaccag ttttaaagaa atcataaaga acattattat catttgatgc acatatcaat
    16081 ttgtttattg gaagacccat ttgtttggca taatatccag ctagaatatt accaaaatta
    16141 ccagttggga caacaaaatt tattttatca tttaaattta tctcactatt gctaactaat
    16201 ttcatatatg aataaaaata gtatgcaact tgtggaagaa gtcttcctat attaattgaa
    16261 tttgcagaag ataatatata attattatcc aatagttttt gattaaattt tttatcagaa
    16321 aaaatcttct ttacacctga ttgtgcatca tcaaaatttc cttttatacc aactacatat
    16381 gtattttttc cagtttgagt tttcatttga agtttttgta ctgggcttac accatcttct
    16441 gggaagaata caattatctt tattttatct atatctttaa atccctcaag tgcagctttg
    16501 cctgtatctc cagatgtagc agtaagaatc acaacatctt tctctaaatt atctttcttt
    16561 atagatgttt taagaagatg aggcattata gtaagtgcca tatctttaaa tgctaaagtt
    16621 ggaccgtgat atagttctaa gaaataagta tcatttactt tatttaatgg agctatacta
    16681 ctacaatcaa acttttcatc atatgcattt tctatacaat ttttaatttc atcttcagta
    16741 aaatcacata aaaatttact aagtacaaaa aatgctatct gagaatacgt taaatttact
    16801 aaatttataa gttcattttt tacattggga aattcacttg gaacataaag accaccatca
    16861 cttgatattc cttttattat tgcttgtgat gcagttactt tttcttcggt gcctctcgta
    16921 cttgaataat acatttttta taatcctcca tatattttaa atttttaatt agctaattaa
    16981 attagataca gaatatttta aaaaccgaat ttaggtaata aaaaaagacc ctcatcccta
    17041 aaaaagggac gagaatctgt ttcccgcggt tccacccaaa ttgattttaa aaaatccact
    17101 tgatacttta acgcaagtaa taaaaacgtc tattcctact aaaatcatta agataacttc
    17161 ggatagaagc tcctaggtag ttttcaatcc ttctaattag ggaaacattt cagctagtag
    17221 tttccttctc tggtaatcga ttcagattta ctcttcctaa tcaatgcctt tcaatattct
    17281 taatattatt aattattcta gtttataaaa cagacaaagt caagtattaa tataaaaatt
    17341 tggaaaaaat attttttaaa ttgtaaaaaa attgtaatca aattgagtat tagaaaaata
    17401 tttttttaac ttttttaaaa gtttaaaaac ctagattttc atagtgatga agctgttttt
//
LOCUS       DAELSY010000079        17356 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.079, whole genome shotgun sequence.
ACCESSION   DAELSY010000079 DAELSY010000000
VERSION     DAELSY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17356
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.079"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(64..336)
                     /locus_tag="KPG09_002332"
     CDS             complement(64..336)
                     /locus_tag="KPG09_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966937.1"
                     /translation="MYDEIIEIYFDYWINEHMYRSILEKDEYYIECWKEVKKINELLE
                     INLEDDKKYLLVELNDLFNSLIEFERELSYKLGFKTGAKFIIELLK"
     gene            complement(586..1920)
                     /locus_tag="KPG09_002333"
     CDS             complement(586..1920)
                     /locus_tag="KPG09_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0966938.1"
                     /translation="MNNSNNLIKNTEAEQSILGSILLDSTIVEELEQMLNEDMFYHEE
                     HKYIFNAIKEINKRNEVIDIITLTEELKRKGHLNNIGGISYITSLSTIVPTVSNFTSY
                     VSIVIEKYKNRNIIDKFNKFNLGEIDDNTLIYQLENDINKANQKLIKKDSSITAISER
                     AFEYINDEMNEGIKTGIKFLDDTIGGLYGGELTTIAAKSGRGKTALALQILRNVIFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNDDEIVKYIQALSIINQENNLY
                     INDSVNTVSGIKREIKSTKPSLVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPVIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALNYCKTMGIE
                     DIRELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGNLFSFEECKKIQEI"
     gene            complement(1933..2676)
                     /locus_tag="KPG09_002334"
     CDS             complement(1933..2676)
                     /locus_tag="KPG09_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966939.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKRIKIKSGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSIIREYDKEAVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYHSKEIVINKETGIYNPDDSQLKW"
     gene            complement(2698..3012)
                     /locus_tag="KPG09_002335"
     CDS             complement(2698..3012)
                     /locus_tag="KPG09_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966940.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            complement(3016..3216)
                     /locus_tag="KPG09_002336"
     CDS             complement(3016..3216)
                     /locus_tag="KPG09_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0966941.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKEE"
     gene            3363..4127
                     /locus_tag="KPG09_002337"
     CDS             3363..4127
                     /locus_tag="KPG09_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0966942.1"
                     /translation="MQIGKNLKKIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYFLQSLFPEEYYSFDEFLNAISNLME
                     DDDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCVSNTELDFSLHPDDI
                     KEIIKNDVLIKQSIDELLDIGLSEENIEAIYDYFSEATDNGSNIRLKKLISQKENMTI
                     KNNDYFKLLQENNELKEKNKKLENTLKEIKKLLTKI"
     gene            4184..4381
                     /locus_tag="KPG09_002338"
     CDS             4184..4381
                     /locus_tag="KPG09_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966943.1"
                     /translation="MNKAYMLKYTYKYPTNLFYGDKKYTLTYIKRIMSKLVYDIDNLE
                     NNSFICVKIQSLFDDIFKVEL"
     gene            4378..4869
                     /locus_tag="KPG09_002339"
     CDS             4378..4869
                     /locus_tag="KPG09_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0966944.1"
                     /translation="MINNTIGEKIKQYRKSINMSRNELANKIGCSVHAIAKYEQNQRI
                     PSLDMIRKISTALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVVHILENYGYDI
                     KTDYKKKFEITDNADTIKPISLSFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            4961..5713
                     /locus_tag="KPG09_002340"
     CDS             4961..5713
                     /locus_tag="KPG09_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG0966945.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYIIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNNLFISDISIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            5703..6869
                     /locus_tag="KPG09_002341"
     CDS             5703..6869
                     /locus_tag="KPG09_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG0966946.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHMQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENRLEILTLEQQKNFIKAIEGHKLEVLFLVALGTGLRLGELLGLKW
                     FDIDFKNSNLTVKRTLQRTYFIDKNGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNVDILKAGELYRNDNYVFCNELGVPIDDKRPLRNLKSILSSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(6985..7215)
                     /locus_tag="KPG09_002342"
     CDS             complement(6985..7215)
                     /locus_tag="KPG09_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966947.1"
                     /translation="MEANKTLLQILYKKIILEFSNQTGKSLEESMDYLYTSETYKLIS
                     EGVSDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(7221..7700)
                     /locus_tag="KPG09_002343"
     CDS             complement(7221..7700)
                     /locus_tag="KPG09_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HBG0966948.1"
                     /translation="MIVYHDSYCVAKTPNIIFLRDSLDFGKGFYVTSIKTQAINWIER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFNSYNLEWLDFILECRNMSDVYMKYDVIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRCEEEV"
     gene            complement(7700..7915)
                     /locus_tag="KPG09_002344"
     CDS             complement(7700..7915)
                     /locus_tag="KPG09_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HBG0966949.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKSYIVEDLIQVLEEKGVM"
     gene            8826..9731
                     /locus_tag="KPG09_002345"
     CDS             8826..9731
                     /locus_tag="KPG09_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P63C domain-containing protein"
                     /protein_id="HBG0966950.1"
                     /translation="MESKEKSILRATHFGIIKIGEKDLNCAVLENGSRIINKSTVFKA
                     FGRTKRGRKKDEIRVPSKPNLPSFIDAKNLSDYISDELEDMLINTIKYKTKNGRIVEG
                     YPAEIIPLICDVYLQARLDNKLAVQQEGLAIASEILVRSLSKIGIVALVDEATGYQYD
                     RDRDELQKILSMYISKEFLPWTKRFPDEFYRQMFRLKNWTYPRPSSKRPGIVGTYTNK
                     YVYDLLPPGVKEELQRVNPTVKPGQRKHKHHQFLTEDIGNDHLKNHLLKVITLMQASK
                     DWKDFNVLFNRAFNLPVQLEIEYDE"
     gene            complement(10405..10754)
                     /gene="ssrA"
                     /locus_tag="KPG09_002346"
     tmRNA           complement(10405..10754)
                     /gene="ssrA"
                     /locus_tag="KPG09_002346"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(10912..11367)
                     /gene="smpB"
                     /locus_tag="KPG09_002347"
     CDS             complement(10912..11367)
                     /gene="smpB"
                     /locus_tag="KPG09_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBG0966951.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            11987..12814
                     /locus_tag="KPG09_002348"
     CDS             11987..12814
                     /locus_tag="KPG09_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG0966952.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            13831..14295
                     /locus_tag="KPG09_002349"
     CDS             13831..14295
                     /locus_tag="KPG09_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0966953.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLS
                     KEDKRKNFISFINDNKELIGFINLLDEGSYVFFGIGIKPDYCGMGIGKEIISLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYKSQGFKIVETQVQKTHLGDGEFFVMRYQ"
     gene            complement(14562..15206)
                     /locus_tag="KPG09_002350"
     CDS             complement(14562..15206)
                     /locus_tag="KPG09_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0966954.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKSLSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPIKRKIR"
     gene            complement(15136..16425)
                     /locus_tag="KPG09_002351"
     CDS             complement(15136..16425)
                     /locus_tag="KPG09_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0966955.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIDEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINLIWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFIMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(16422..17294)
                     /locus_tag="KPG09_002352"
     CDS             complement(16422..17294)
                     /locus_tag="KPG09_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0966956.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILDSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
BASE COUNT         6288 a         2516 c         2043 g         6509 t
ORIGIN      
        1 tagtacaact tattaataat ctcgatttac tatttaatta ttctattgag atacacaaat
       61 taattacttc aacaactcta ttataaattt tgctcctgtt ttaaatccta atttataact
      121 aagttctctt tcaaattcta ttaatgaatt aaacaaatca tttaattcaa ccaacagata
      181 tttcttatca tcttccaaat ttatttccaa taactcatta atttttttaa cctctttcca
      241 acattcaata taatactcat ctttttctaa aatacttcta tacatatgtt catttatcca
      301 ataatcaaaa tatatttcta taatttcatc atacatttta gtacctcctt gactttcatc
      361 aggtacttaa tatatactta agttgctagg ttagtgtaca ttaagtacct aatttataaa
      421 ggtagttgtt atatttatat ggcaactacc tttttctaat aagcatttaa atctaaactc
      481 aatatatttt aaatctctat tacatctaat taagttttat atatttacca ttcaatactt
      541 cttgaaacat aatttatcta ttacttaata aatcatgctt cttttttata tctcttgtat
      601 cttcttacat tcttcaaaag aaaacaaatt tcctatatac caatatggtc taaatcctga
      661 ctcaccatct ctatttttgg caacaataat ctcacttaaa ttagcaccaa aacctttttc
      721 tcttcgtctc aactctctta tatcctcaat tcccattgtc ttgcaataat taagagcctc
      781 tttttctgta ggtctatgaa tatatataac tgaattagaa tccatatata tctgctttga
      841 gcttctcatt actctatccc ctttaggtct ttcatccttc aattcatcat ttaattgagc
      901 aagctgaata actggtatat taaaatctaa agtaatcttt tttatttgcc ttgataagtc
      961 agcaaattta gcttctccta cttctttacc ttcataatcc aaaatttgca aataatcaat
     1021 tatgactaag ctaggcttag tactctttat ttctctcttt atccctgata ctgtatttac
     1081 agagtcattt atataaaggt tattttcttg atttattatg cttaaagctt gtatatattt
     1141 gactatttca tcatcattta gatttctatt tttcattttt acaactgata ttccagttag
     1201 tgaggctata tttctaaaca gaatctgtat atcactcatt tctccactta taaaaagtac
     1261 tttctttcct tgaaatatta catttctaag tatttgtaaa gctaatgctg ttttccccct
     1321 tccacttttg gctgctattg tagttagttc acctccatat aaacctccta tagtatcatc
     1381 taagaacttt attccagtct taataccttc attcatttca tcatttatat attcaaaggc
     1441 tctctctgat atggctgtta tacttgaatc cttttttatt aatttctgat tggctttatt
     1501 tatatcattt tctaactgat atataagagt attatcatct atttctccaa gattgaactt
     1561 attgaattta tcaattatat ttctattttt atatttttca ataactatac tgacataact
     1621 cgtgaaattt gatactgttg ggactattgt tgacaaactg gtaatatagc ttatacctcc
     1681 aatattattc aaatgacctt ttctctttaa ttcttctgta agagttatta tatctattac
     1741 ttcatttctc ttatttattt cttttatagc attaaatatg tatttatgtt cctcatgata
     1801 aaacatgtct tcatttaaca tttgctctaa ctcttctact attgttgagt ctaataaaat
     1861 gcttcctaat attgattgct ctgcttctgt atttttaatt aagttgtttg aattatttat
     1921 aatataacct ccttaccact ttaattgtga atcatctgga ttatatattc ctgtctcttt
     1981 atttatgaca atttccttgc tatgataatt gccctctagt atcttggtaa aattacttga
     2041 attcattatc caatcaaaat ttgctttcca tcctgtatta ttatttccat tcagaaaact
     2101 tgttttacta actatattta taacctctaa tacagcttct ttatcatatt ctcttattat
     2161 tgaatttatt ctcctctttc ttgtgtctgt tagtttcttg acctttgaaa gttcaataca
     2221 agttgagtta aatttgctta taatttcact atatataata ttattttcat tctttatatt
     2281 cttgattgtt gttgattgat tgttaatcgt ctgttgatta ggttgttgat tttctatgtt
     2341 ctcttggtaa agctcccagt tttcaatagt tatgactata tatttgtttg ttgtttcgta
     2401 tgttagaaaa ccagcatttt taaaaaccaa caaacaactt ctaaccattt gataactaac
     2461 tcctttacca catctttcag ctattttgct ataagaagaa ataaattgtc cagactttat
     2521 ttttatcctt ttattatcta taaaaacatg attttcttta tgactagcca taagtaatag
     2581 agttattaat attacttttc tttctggttt aatatctttc caaattgatt tatccaacaa
     2641 ctccctgtaa agctttatcc accctttatt gctcaattgc aaacacctac tttaagacta
     2701 taacttgata ccatttgcat tttcaataaa ccaatcatcc aatttgtttt ttattatgta
     2761 tactcgatta tttatccata tacaagggaa acctttattt ttagagtgac ataactgcct
     2821 aaattttgat tctcctatag gataaagctt agatgcttct ttacatgtta taagttcagc
     2881 tttatttctt ttcgcatttt caataatttt atatccttgc tttttttctt gtgcttcttc
     2941 ttcattcttt acaataatgt ttaaagtagc ttgtaatgtc tttaaaactt ctaaattatt
     3001 attctctttc atattttact cctccttact aaaaaataat tcttctactg ttgtatctaa
     3061 agctctagat agtttaatca tcaaatcatt gcttggatta ttagccttac ctgtttccaa
     3121 gtttgacaaa taatatcttg atataccaac tctatctgcc agttcttgtt gctttaaacc
     3181 ttttttaatc ctaaatattt ttaaattgat tcccatactt tttcctcctt ttttctagtt
     3241 tacattcgta aactttacat tttaattata atgtcgacat atgtgtgctg tcaataagaa
     3301 tatattattt ttctgttctc atttgtgtac ttttgggata tactttatac gaggtgataa
     3361 tattgcaaat tggaaaaaac ttaaaaaaga taagaaaaca aaaagaacta acacagattc
     3421 aacttgctga aattagtggc atctcaagaa atgctctaat aaattatgaa aatgataaaa
     3481 gaatacctag tatagatacc ttatcaaaac tcgctaaagc tttaaaaatt gaaaagacta
     3541 tcttttattt tgatattgaa aatgaagtaa tgactaatga agatgtttta tattttttac
     3601 agtctttatt cccagaagaa tactattctt ttgatgaatt tttaaatgct atttcaaatc
     3661 ttatggaaga tgatgatatt aggattacct caagtttatt gctaggtgct tggtctgata
     3721 aagaccaata ttataaactt ggtgaaaaat tagaactaaa tgaaaatcaa gtttataatt
     3781 ggatactttc agattgtgta tctaatactg aattagattt ttcattacat cctgatgata
     3841 taaaagaaat tattaaaaat gatgttctta taaaacaatc aattgatgaa ttattagata
     3901 ttggtctatc agaagaaaat atagaagcaa tctatgatta ctttagtgag gcaacagata
     3961 atgggagtaa cattcgacta aaaaagttaa tatctcaaaa agaaaatatg actattaaaa
     4021 ataatgatta ctttaaatta ttacaagaaa ataatgaatt aaaggaaaaa aataaaaagc
     4081 ttgaaaatac acttaaagaa ataaaaaaac tactaactaa aatataaaat aatatcaata
     4141 ctatctaaag caatgataaa aaactacaaa tggagttaca attattaata aagcatatat
     4201 gctaaaatac acatataaat accctactaa tttattttat ggagataaaa aatatactct
     4261 aacatacatt aaaagaatta tgagtaagtt agtatatgat atagataatc tagaaaataa
     4321 ctcctttata tgtgtaaaaa tacaatcatt gtttgatgat atatttaagg tggaattatg
     4381 ataaataata caatcggaga aaagattaaa caatatagga aatcgataaa tatgtcaagg
     4441 aatgagctag ccaacaagat tggttgttca gttcatgcta tagctaaata tgagcaaaat
     4501 caaagaatac ctagtctaga tatgataaga aaaatatcta ctgctttatc aattgccata
     4561 aataaactca ttgttgaaga aaatgattta gatactttaa caaataataa tattaattta
     4621 aaagtatttg ataaagagtt ggatttagta gttcacatat tagaaaatta tggttatgat
     4681 ataaaaacag actataaaaa aaagtttgaa attacagata atgcagatac aataaaacca
     4741 attagcttaa gcttctctga cttcaaagac ttctccaaaa atctaaattg ggtgataaat
     4801 ggtcttgttg aaaattttat acaagaaaac tctaatggtc ttattgaaga tgaggatgaa
     4861 gaatattagt attgcacatt ttcaaaatat gggtttacct attaactttt atacattcat
     4921 agttaataaa taaaaattag atatgaaagg agctaaaatc atgaataaat catatatacc
     4981 aaaatacaca cataaattcc ctacgaatct acctcatgga gataaaaagt atactttaac
     5041 atacattaga agaatgataa gtgaatttgt atatgatatg ggtaacctag aaaataaccc
     5101 cttcacattc ccagaagtac aaacattact tgatggtata acagtaggtg gacataaatt
     5161 aagtgaccaa aatcaaatac taaatataaa atcatcatgg gattatataa taaaattaag
     5221 taacaaagaa aacttatctt taaataaaga tacaatttgc tctatacata aaatagttgc
     5281 aaaagatgaa gctctaattg ttggagattt tagaaacggt aacataggta tagctggtac
     5341 tactcaatat gagtgcatag aagccactct attaaataat ttatttataa gtgacataag
     5401 tatcataaat aaaataacta accccttaga aaaggctata ataataaatt tatggttgtc
     5461 ctattgtcaa ttcttctatg atggtaacaa aagaactgct cgacttacct caaacctaat
     5521 acttataagt aatgatatag gagttctatc aataccagcc aaacataaac aagaatacaa
     5581 cacattaatg ctaaacttct atgaaacact agaagctgat gaagttataa aattcttatt
     5641 agagaaatgt attacattct tcgatggata taactataaa tcatataagg agttgtttaa
     5701 aaatggcaac taaaagagct aatggagaag gctctatcgg aaaatataaa gatggttggc
     5761 gttctcgtat aatgattggt tataatgaag atggaaaacc aataagaaaa gaattttatg
     5821 gtaaaactca aaaggaagtt aaagataaac ttgatgctta taaaaaacaa tattatttaa
     5881 gttctgatat ttcagatgat aaaataactt tagagcattg gttttacaat tggttgtttg
     5941 aatatagaat aaaagattta aaaccaaagt catttgaaag atatgaaagc atctatcgta
     6001 attatattaa agatactgat ttaggcaatg ttaagttaaa ggacttaaga gtatctcaca
     6061 tgcaaaaata ttacaataga ttgcttgatt ccaataaaag tatacctact ataaggcaaa
     6121 taaacactaa actgaaaacc tgcttatcag aagctgaaaa acaaggtctt atacaacgta
     6181 attattgtac tatggtaaat ataccagttg agaaaaagga aaataggctt gaaatattga
     6241 ctcttgaaca acaaaagaat tttataaaag ctatagaggg gcataaatta gaagtattat
     6301 ttttggttgc tcttggtact ggacttagat taggagaatt attaggactt aaatggtttg
     6361 atattgattt taaaaattct aatttaacag ttaaaagaac ccttcaaaga acatatttta
     6421 tagataaaaa tggaaataga gagcttaaag tattagagca agaaccaaag acatcaaatt
     6481 cttacaggac tgtacctatt ccaaaagatg ttttaaataa attgaaagag cataaagtaa
     6541 atcaaaatgt ggatatatta aaggctggag aattatatag aaatgataat tatgtatttt
     6601 gcaatgaatt aggtgtccct atagatgata aaagaccttt aaggaattta aaatctatat
     6661 taagcagctt agatatagaa cctataaagt ttcatggtct tagaaaaact tatgcaacta
     6721 gactttttga gaatgatgtt cctcctaaaa ctgtacaagt tttaatgggt cattatgata
     6781 tatcaataac tttaaatata tatactcaag ttatggaaga taagaaggtt gaagctgtag
     6841 aaaagttgga taaaatattt tcattgtaaa aatcaaatac ataaaaatac tatgatttca
     6901 tatagttaat tacaaaaccc aaataaggat acaaattaat tttatattat cttgtatcct
     6961 taattcgtat ctagttttaa ctatttatct tggatagcct ttatgataca ttaatccatt
     7021 ttctagcatt aattcttgag ccaagtatat atgaccctta cagtgcatat ctgatacacc
     7081 ctcacttata agtttatatg tttcagaagt atataaataa tccatacttt cttccaaact
     7141 cttgcctgtc tgattagaaa attctaatat aattttttta tatagtatct gtaataatgt
     7201 tttatttgct tccataagca ctatacctcc tcttcacatc taaactttaa atatttatct
     7261 attacatttt gattagttat acatatttgt tcatttggct tatagtattt taatcttttt
     7321 aaagcttcat ctttttcaat aagattatct tgatacagtt ctattgtatt gtaaatctta
     7381 tcatcagcaa ttccacctat tataacatca tatttcatat atacatcact catatttcta
     7441 cattctaata taaagtcaag ccattctaaa ttataactat tgaagatttt taccttgcag
     7501 ttttctttta atttatcaat atctagagaa tagctattta aatatgcttt tttacccctt
     7561 ttcttaaaac gttctatcca gttaattgcc tgtgttttta tactggttac atagaatcct
     7621 tttccaaaat ctaaagaatc tcttaagaag ataatattag gcgtttttgc cacacagtat
     7681 gaatcatgat atacaattat tacatcacgc ccttttcttc aagcacttgt attaaatctt
     7741 ccactatata acttttactt tgagaatgta gtgcttcata gcaaggtatt atatagttat
     7801 ctaaaatatc agtatcatgt ttaagaagtt tataaactcc ttctgaatct atattcaact
     7861 tttctgctaa agcttcaatg caaaaaatag aaaattctag ttttctttca tccattacaa
     7921 acctcaactc ctatccactt atataatata tatattttta ttaattaata acttaaacca
     7981 tcttatgttt ataaaaatat tatatcataa agtagatatt aaaattctat gttacataat
     8041 atcataaaca aataaagact agcatttcca aatatttcat catttatttt ttaactcagg
     8101 gaagatatac cacttgaaca atatcctcaa ctgatatttt gatatattaa atttctatta
     8161 taaaaatttt attctaatta taaatcatat agtaatatac attcattact ttataatttg
     8221 aaacctctta cactaataaa atttgaaagt gctcattatt caatacaaac atgcctaaaa
     8281 ttatatttta atttttacac tatcaaaaac atatatatta aatttgaccc acttaaatac
     8341 agtaaattaa tttctacaac atgatataat taaattatga caaaagactt tgatatttat
     8401 aaaaactttc caacagatgt ctcttataag atttttttat ataatcttaa attaaaaaac
     8461 tcattaatat gcccctattg ttattctgat aaaattagta cttataatga tttatttcat
     8521 tgcaatattt gcaatagtaa gttttcaatt acaactaata ccattatgca taaaactaaa
     8581 ctagactaca gaaaatggct acttgctatt aatttattta tatttgatga aaatattagc
     8641 tatagaaaat tatcaaaaaa tatcgttgtt aataaaaata ctgcgtacaa gatgataaaa
     8701 aaactcaatt atctatatac atattttaaa attgatatta ttagtcttat aaatactaat
     8761 ttatctaatg aagagatact aagtaatatc ttacttatta aaataaaata aaggatggtg
     8821 ttaaattgga atcaaaagaa aaaagtatat tgagagcaac acattttggt attataaaaa
     8881 taggtgaaaa agatttaaat tgtgctgtac tagaaaatgg ttcaagaatc ataaataaaa
     8941 gtacagtatt caaagctttt ggaagaacaa agcgtggtag aaaaaaagat gaaattcgtg
     9001 taccttctaa gcctaatcta ccaagtttta ttgatgcaaa gaatctatct gattatattt
     9061 cagatgaatt agaagatatg cttataaata caataaaata taagactaaa aacggaagaa
     9121 ttgtggaagg ttatccagct gaaataatac ctcttatatg tgatgtatat ttacaagcaa
     9181 ggcttgataa taaattagct gttcagcaag aaggactagc tatagcaagc gaaattttag
     9241 taagaagttt atccaaaata gggattgtag cattagttga tgaagctaca ggatatcaat
     9301 atgatagaga tcgtgatgag ctgcaaaaaa tattgtctat gtatatttct aaagagtttt
     9361 taccttggac aaaaagattt cctgatgaat tttatagaca aatgttcaga ttaaaaaact
     9421 ggacataccc tagaccaagt tctaagagac ctggaatagt tggaacatac acaaataaat
     9481 atgtttatga tttattacct cctggagtta aagaagaact tcaaagagtt aaccccacag
     9541 taaaacctgg gcaaagaaaa cataaacatc atcaattttt aacagaagat ataggtaatg
     9601 atcatttaaa aaatcattta ttaaaggtta taaccttaat gcaagcatca aaagactgga
     9661 aagatttcaa tgtgcttttt aatagagctt tcaatctacc tgtacagctc gaaatcgaat
     9721 atgatgaata atagactata gcctttaaca acgctttaaa tacttttaat ttatattata
     9781 aaaatagaat taacttatat cataaaagta cttattagta taggtacttt tatgatatat
     9841 tatttaaatt ttatatataa tatattgtct atatacaata gtcttgttag tattaaactt
     9901 aataattcta tgtgtttatt atcttaacat ttttcaattc cttttttaca tcttattttt
     9961 ttaaatacaa ttaaaaaatt taaattagca tcccacatag ataataaatt tgttctatac
    10021 ttatacataa tcttatagga ttctgtatat cattataact agtaattact ctaatagatt
    10081 aagtagcact tatatgcttt aaacttcttt atccttttaa tcataagttt aaacaagtta
    10141 tatatttcct agattgaaat aaaaagcata atataaaaac aattcatcag tataaaatat
    10201 ttattgtatt atcatcaaat tcactatatc tctaaaagta ttctttatat gttccatgga
    10261 ttaatcaaat gcaccccaat ataccccaaa aaaatctctt tttctgtggt tggggcacgg
    10321 ttggtggatt tcatctccaa aacaaaaaac actagtcaat aaagactagt atttccaagt
    10381 atttcatcat tcttttttaa aaattggtgg agacgagggg agtcgaaccc ctgtccgcaa
    10441 atcttttcct tgctgcatct acatgcttag ccaacttcac taatatctca cctcacatag
    10501 cgccaagtgg caggctctat gcttagccag tcccaattaa tctacttcag gtcgagacac
    10561 ccctaaagta gttccctgca taatgacgtc cagtcttagt cggcaggagg actaagaggg
    10621 acgagcagca actaggctgc tatcgcgaaa ttatcgtctg cgtttatttt aaagttcccc
    10681 agtttttacg tgacccagag taacacgaca tgcagcctca agttccaaac ccacgtcgaa
    10741 acctttacgc ccccttgagt agtttatagt ccagtagact tttcgcaaac tttatcacca
    10801 tttttattaa atttttaatg tatatataat ataatacttt taagaataaa aatcaatgtt
    10861 atgttctaga taataaagaa ttttattcta aaaaatacag ttttatctag tttaatactt
    10921 acctgtcatt tctctttcta tacgtctttg tgcatctttc ttagccatat cttgacgttt
    10981 atcatatagt tttttacctt ttgctaaggc aatctctact ttaactttac cgttttttag
    11041 atacaaacta agtggtataa gtgaataccc ctttatagta gttgctccaa taagttttct
    11101 tatttcatgc ttatgaagta atagttttct aactcttaaa gaatcaacat tgaatatatt
    11161 tccttgctca tatggactta tatgaacttg ttttaaaaat acttcactat tatctacaga
    11221 tgcaaatcca tcacttaggt taactttgcc ttgtcttatt gattttactt ctgttccttt
    11281 taattcaatt ccacattcat aagtttcttc tataaaatat tcatgtcttg ctcttttgtt
    11341 tgttgctaat atttttttac cagccatata aatctcacct tctttgttag aaatttattc
    11401 cataaatgtt agaaatttac taaatattta atgttgccaa attatttgac taacttgtat
    11461 gataacatat ttaggcattt tagtcaatat ttcactgata tattaagtta gttaggctat
    11521 atataattaa gtaatataaa gatatgaaaa tagctaaaaa actacttcga atatacttaa
    11581 tactaaacta actttaacca gtaacactga tttctaaatt tcaaaacaaa aatattacat
    11641 attataaatt ttctatcttc tatcttctat cttctatctt ctatcttcta tcttctatct
    11701 tctatcttct atcttctatc ttctatcttc tatcttctat cttctaaata attttaacat
    11761 gcccataaaa tataataccc acaaatgata aataatactc aaaatatcta ttgaaataac
    11821 tatacatcct taaataactt aacttaaatc taaacatttc aaaaacttct taaaaatatg
    11881 taaaaatttg atgataaagt agcgattttt tcaaaaaaaa tgtaatatag agttatatct
    11941 tataatattt tagagataaa ttttatcata gttgggagaa atttgtatgg atttaagcaa
    12001 aaaagtaaaa attttagaaa atgcatcaga tggcaaaagc gaatttgaaa tattagaatt
    12061 tgaaaaactt gagggagctt ctagcccatt aatggcaatg ggattatatt ttgctagaga
    12121 agctggtttg aaagctagac aaattaagat aaaattaaat aattcatcaa taaaaacaga
    12181 agctggtgca ttatactatt ataaaggaaa tatatcttct gaaactaaga ttggtggtgt
    12241 tggaggtctt tttaaaaaag gaatatcagg agcacttact aatgaatcaa tgatgaaacc
    12301 tgtatacaaa ggtactggag aagtttattt ggaaccttct tttagacatt atttatttat
    12361 gagtcttgat aatgattcta taattgttga taaaggatta ttctattgtt gctctgatac
    12421 aatagaagta aaagcattta cacaaaagaa cttctcatct gctttacttg gtggtgaagg
    12481 cttcttccaa atagaattaa gtggtactgg tgtaatcgtt ctggaatgtg ttgtacctca
    12541 aagtgaaatt gtagagtatg aattgaaaaa tggagaagaa ttaaaggtag atggtaactt
    12601 tgcaatagca agaacatctg gggttaattt ttctgttaca aaatcagata aatctttaat
    12661 tggttctgct ttaaatggtg aaggtttctt aaatacattt actggtaatg gtacagtttg
    12721 gctagctcca actcaagtaa tgtatgaaaa actaagtttt ggagcaatgc ctactaatga
    12781 taatatgaat aataaagttt ctaagcaaaa atagttgttt tataaatatt tattaaatca
    12841 ttattgattt tataaagtca ctataaattt tattagatta ttattataaa atcagagtgt
    12901 ggaaatgttt atgtgtattc tttaaataaa aatttccaca ttctgtaagg ttattatact
    12961 tgatatataa aattcaattt aggaacatag tctaacattt ataaacatat taaggtaatg
    13021 atttctcttg gtaattattc tcatatctta ttctttgatt atatttatct tattcctagc
    13081 ccttatgtca tattatattt cttatcttat tatttataat tagcattaac ttatctaaaa
    13141 tcttgtattt ttttaatagt aaagtttaca tctatattaa aaagtttaca tctatattaa
    13201 tttcataaaa caatcaaggt agtcaagaga ttcaacttta tttgatttta aaaatttaag
    13261 tgaatttgtt cttatacaca tctttttaaa attatatctg tagaaaatat catttagagc
    13321 tataattaaa ttattaaatt ttagaaaatt tattaaaaaa tataagttaa attaatattt
    13381 ttaaagattc ttttttataa ctactagaat actaatttag tagttattgt atctctaaag
    13441 tttaagcatg gatagatata catttgataa tttgcctata atttacctac tacttggagt
    13501 attggagcaa atacaatata ttaataataa gttcctaaaa ccaataaatt aactaataca
    13561 cataaagata ttgatgttga ttatacaact ataagaaaca gatttaacag aatttaatca
    13621 agctaagaaa ttacatatat cgtgatgttt ttgaattaga aggtaattca aaattagttt
    13681 atagtcttct gtgaatccaa taaaaagtac agggtacaag aattgttagt aaaattttta
    13741 aagattttat ggatagatat aaatagtttt acttattttg taaaatttaa aataccatta
    13801 attaccttaa atattttaat aggaggacat atgaatttaa ataaagttaa atttactgaa
    13861 gaacatgcta ttgaagttac taattggaaa tatgaaggta aatattcaat atacaaccta
    13921 cctccatggg aggaaattaa aaagaagaat ttttcactat caaaagaaga taaaagaaaa
    13981 aattttataa gttttataaa tgacaataaa gaattgatag gttttataaa cttattggat
    14041 gaaggaagtt atgtattctt tggtattggt ataaaaccag attattgtgg aatgggaatt
    14101 ggcaaagaaa taattagcct aggattacaa gaatgtagaa ataaatatag tactaaacca
    14161 attgtattaa atgtaagaac ctggaacatg agagctgtaa aatgttataa gtctcaagga
    14221 tttaaaatag ttgagactca ggtacagaaa acgcatttag gggatggaga attttttgtt
    14281 atgaggtatc aatagtttat aacactagtc atattgctaa tatgacaatt aaaatgaaaa
    14341 aatacatgtg tgtatgtcat agcaataagt gtctttgaaa tataaatttg agagcctttg
    14401 ataaaattcg gtatacatat atattttata ctacagatat ataaatctat ctacatacat
    14461 aaagagattg taagtaggtt tgtgtatata aaaataaatt tatatttcat aaatgtactt
    14521 tttatttgta ttacctttat tttatctaag ctctcatttt attacctaat cttccttttt
    14581 attggtttta ttaagagaga tgcaattatc aacacaacac ttccaagtat cagccaatat
    14641 ttctcatcaa aactaccttg actcatagac attataaaaa attttccaac atacttgcct
    14701 ttagtcatca tatttaaaat taaatatcct atagatatca tgtaacctat gattgtatta
    14761 ttaaataaat ttgagaaaaa cattcctaaa cttcctataa acatagctga tgcgaaactt
    14821 ccaataatat actcaactac tggaaaatta catccaagat atattaaaac atttacaaaa
    14881 cctatgatta ataccactat acctataatt gacatcagta tcctaattaa ttgtacaata
    14941 acttgagata atgattttga agatactatc tcatcaattt cttttgattg ctctagcgaa
    15001 aatacaggta ccaacattac tatccctatt attgcaacat aattttctaa acatatagct
    15061 gttgaaattc tgtctaaatt agctactcct tgtattagag gaatcataca tagcaatata
    15121 attgaacata ttaaattagt taaagtgctt aatttgaagt tttcttttat tagaactaaa
    15181 cagttttgaa aactttgccc aaacatcaag attccccctt cttttttgct catataagaa
    15241 tatagctaac aaaacaagca ccaatgctcc caagcttatc acaattctat taatcataat
    15301 ttgattgaag ttttgtataa taatatcccc atttaataca ctattatgtc ttatcattag
    15361 ccccattgtt gaataatcac ctttgagtcc tgtagaattt atactgtaaa gccaccaaat
    15421 taattgcaca ataattgcta ctggtgtatc tgttaatata gtaatactca ttcctacagc
    15481 tgtaactatc ataagagttg gtagtatcca ccataatgta tattttataa atgctaagtt
    15541 atcaatagat atacctttat ctttagcaaa gaaaatcaaa ggaagtaaat ctttcatacc
    15601 taaaattaat acaggcaata tcattataaa gacaagtgca aaatatcttg ataaaactat
    15661 tttgcatgaa gatacttgtt ttgaataaat aatctctctc attcttgaac gcttatcttt
    15721 aagtgacata aaaactgcca caaatactgg aaatatactt aaaactatac ctatataatc
    15781 gcaaaaaatt cttgcatacc cattactaac tttatctttt tgtattgtat agttgtaatt
    15841 cttcatagct tcatcatagg taactggaac tactccaaat ctttgtaact tgtcaaaagc
    15901 atatgcagac cctcctccta aaatgtcgtc tactctttgc attatttctt caaatctttc
    15961 aagagatact ttagaataat ctatttttat actttcatca gttttccaaa ttaaatttat
    16021 ttcgctaata cttttaccag ttatttcttc tatatatttt tctatggtct tcgttttatt
    16081 ttggtctaat tttatatact taactacccc tgttggatac gttgtatatg tgtttttttg
    16141 atattctcta attaggttta tagttgcatt tggaatgacc ttttctgttt tcatagattc
    16201 tttttgacca tatggatatt gattgtgtac agagtaatat tcgtcaatat tctctacttt
    16261 ttgtggagca actaatggat ttccatcatt aggaatattt ttacttacat catttgaaac
    16321 tgaacttcca agttgagaaa tatagaaaaa aactaaaact acaataaaaa tataatatac
    16381 catactcttt aatatatttt tacattcttt tataaataga ttcatatatt atctcctccc
    16441 attataagca tatagccatc ttcaatagtt ggattacaat ttactgctgt ctcaaaaggt
    16501 ttatcatctg atataatcct aagtatagcc tttccaccat gagtcaacat tcctataact
    16561 ataaatctac tttttatatt ttcaatgtct tttttatcta cttcaatact atatactttc
    16621 ccttcagcta gttttgctaa tgcttctgtt gagccattgt atatgacttt tccactatcc
    16681 aatatagcta cgttttcaca tgtagcttct atatctccaa caatatgtgt agataaaata
    16741 acgattctat catttgcaat ttcgcatagt aaatttctaa atcttatacg ttcttctggg
    16801 tcaagacctg ctgtcggctc atccactatt aaaactctgg gattatgaat taatgcttgt
    16861 gctatcccaa gccttctatt catacctcca gataaagatt ttactttcac attttgataa
    16921 ttagtaaggt ttactttgct taataacttt gctataattt ttcttctttt tcttagtggt
    16981 atatccgata aaactgctaa ataatccatt gctttatata ctgtcatgtt aggatacatt
    17041 gaaaaatctt gtggcaaata accaaccata tttctaattt ccttagtatt ctcaatagga
    17101 actccacaca tatgtattga accttctgtt tttgataata aagtggctaa aattctcata
    17161 aatgtggttt ttcctgctcc atttcttcct aaaagaccaa acataccact gtcgatttca
    17221 agatttatat tatcaagagc ttgttttttc ccaaatcttt tactaagatt ggatactgct
    17281 attttcattt ccatatataa atacaccccc agaaataatt ttatagaatt ttatttttat
    17341 aaatttttac tgtatt
//
LOCUS       DAELSY010000080        17265 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.080, whole genome shotgun sequence.
ACCESSION   DAELSY010000080 DAELSY010000000
VERSION     DAELSY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17265
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.080"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            119..289
                     /locus_tag="KPG09_002353"
     CDS             119..289
                     /locus_tag="KPG09_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0966957.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            complement(465..1004)
                     /locus_tag="KPG09_002354"
     CDS             complement(465..1004)
                     /locus_tag="KPG09_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG0966958.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAARKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(1143..1775)
                     /locus_tag="KPG09_002355"
     CDS             complement(1143..1775)
                     /locus_tag="KPG09_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBG0966959.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            1911..3824
                     /locus_tag="KPG09_002356"
     CDS             1911..3824
                     /locus_tag="KPG09_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG0966960.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            3840..5216
                     /locus_tag="KPG09_002357"
     CDS             3840..5216
                     /locus_tag="KPG09_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0966961.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(5356..6006)
                     /locus_tag="KPG09_002358"
     CDS             complement(5356..6006)
                     /locus_tag="KPG09_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBG0966962.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            6477..8489
                     /locus_tag="KPG09_002359"
     CDS             6477..8489
                     /locus_tag="KPG09_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0966963.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNALIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIDDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
     gene            8858..10078
                     /locus_tag="KPG09_002360"
     CDS             8858..10078
                     /locus_tag="KPG09_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0966964.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISSTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            10115..11464
                     /locus_tag="KPG09_002361"
     CDS             10115..11464
                     /locus_tag="KPG09_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0966965.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYSLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWFIRIISWSTL
                     AVGFATALGSFWPESATEYKGCIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFVIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     IALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            complement(11596..11889)
                     /locus_tag="KPG09_002362"
     CDS             complement(11596..11889)
                     /locus_tag="KPG09_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966966.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYDKDGNL"
     gene            complement(12373..12576)
                     /locus_tag="KPG09_002363"
     CDS             complement(12373..12576)
                     /locus_tag="KPG09_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966967.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
     gene            complement(12627..12878)
                     /locus_tag="KPG09_002364"
     CDS             complement(12627..12878)
                     /locus_tag="KPG09_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HBG0966968.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            complement(12894..13964)
                     /locus_tag="KPG09_002365"
     CDS             complement(12894..13964)
                     /locus_tag="KPG09_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HBG0966969.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            complement(13993..15414)
                     /gene="ccpM"
                     /locus_tag="KPG09_002366"
     CDS             complement(13993..15414)
                     /gene="ccpM"
                     /locus_tag="KPG09_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HBG0966970.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVNKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDYISIDDFISKNET
                     LKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIKDESMSPLSRIER
                     ALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSESSKMGMI
                     GNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCD
                     RIRHSTENNMKDYCVLKSLGYQY"
     gene            complement(15436..17208)
                     /locus_tag="KPG09_002367"
     CDS             complement(15436..17208)
                     /locus_tag="KPG09_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0966971.1"
                     /translation="MENGKFQFFKGVRNCFNMIYLHDKRYLFISMFMAIFQGIVPSIS
                     LLVSQNIINNMQTFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKHIE
                     TMMLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLIVV
                     LSQFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYAYK
                     EIMVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFIIIQ
                     GILKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQDLFYTDLLFKFLDYNCKEDSI
                     EHIKIFEKIGDINKIELINISYKYNHSSEYSLKNINLTLNKNDPIAILGKNGSGKTTL
                     LKILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGDVY
                     KSNLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMKDA
                     DMYIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFITHHIQHAKKITNNIVVLENGKL
                     LESGRHDELMDKKGVYFNLFCNCK"
BASE COUNT         6369 a         2378 c         2153 g         6365 t
ORIGIN      
        1 tttaaaacaa gaatatcata ataaagtttt tgttgtagaa ttaatttaat tgttatattc
       61 aataatattt gttataatgt tattgaatta caatacatat aaaagggagg atttcaaaat
      121 ggcatataaa attactgacg cttgtataag ctgtggtgct tgtgaggctg aatgccctgt
      181 tagctgtata tcagctggag acgacgcata tgttatagat gctggttctt gtatagactg
      241 tggttcttgt gcaggagctt gtcctgtaga tgcacctcaa ccagaataat aattcgttat
      301 tcataaagag actgatttta ttttctaaat actaatcagc ctctttttaa ttatgtatgg
      361 ttcaagtaat attatttata tattttatta tgttgctacc taattcatct taaaatactg
      421 ttagtacata tctaatactt ttaactttaa tctaatttat aattttaata ctcctcataa
      481 aatctttcat atttattaaa gcaatcaaga catactttct ttccttcttc aatccttata
      541 tattgctcag atgcaaattc accacacact tcacatttaa gagatttaaa tattcttgct
      601 ttttcaggaa gtcttaactt agtatctgta atctcaaata tttcttcaaa atcactttct
      661 aataagtact ttttgtatcc ttctctatcc atttgaaatg gctttgactt tacatatatt
      721 cttatagatt ttccattttc cctattaaag aaatgatata ctgatttccc agtcatccta
      781 agtagtaaat ttcctttacc cattgtgcaa cttagcacga cttgtatacc atctattcca
      841 caagcatcat tttcagaaat acatacaatt tcctcatctc ctgactgttt acatcctaaa
      901 tattttcttg ctgcaagtgc tgctttaaat cctatagcta accctggaca ttcatgacca
      961 tgaaattcaa ttgccttttt ccataattcc tccacaaaaa ccactctcct taatattgtt
     1021 tatttccata cattatttaa atttttatcc taatatttca aaaagccaga ggatttaata
     1081 ttaaatcttc tgactttctc aatactcttc acaactgctc attattaata tgtttaagtt
     1141 gtctactttc cttctttcat taaatacgat aaggtaaata aactttctgt taagtttaca
     1201 ttttcaacta taacaccttt cggaacttct aaatctaaag gtgcaaaatt ccatacacct
     1261 ttgattccag cttttacaac tctattaata acttcttgtg ctccattttt aggtatacat
     1321 aatacagcaa tatctatatt attgtttttt ataaagtctt ctaaagtatc tgaatctaat
     1381 acttcaaact ctcttatctt taaacctatc attctaggat ttgcatcaaa taaagctttt
     1441 atctcgaatc cagcttttct aaatcctgca taattggcta tagcttgtcc taagttacct
     1501 gctcctacaa gaactgcgtt gtatggtcga tccaacccaa gaattttacc tatctcagta
     1561 tgaagagctt ctacattata accatatcct tgttgtccaa atccaccaaa gttgtttaaa
     1621 tcttgtctta tttgagaagc ggtaaaccct attatatcac tcaattcttt agaagatatt
     1681 ctttgtatat ccctatctaa taagtctcca agatatctat gatattttgg gagccttctt
     1741 ataactgcca ttgatatatt tttatttccc aacatcttca cctcctaaaa gtgtcctctt
     1801 ttttgtttga ttcacaataa aatatttctt tttcaaaact aatacatata accttgatta
     1861 tattatatca aaacattata attttagata gttcaaggag gtagaaattt atgattgttt
     1921 tgtcatgtaa taacttaaat aaaagttttg gtatagattc tatattagaa aatattagtt
     1981 ttacagtaaa tgaaggcgat aaaattggta taatcggtgt taatggtact ggaaaaacta
     2041 ctttatttaa aataatttca ggaatttatg gatatgatag tggagatatt tatacttcta
     2101 aagattgtga aatcggatat ttagaacaaa ataccaattt ttattcggat aatactattt
     2161 tggaagaagt gctagaagtt tttaaaaatt tgatagaaat ggaatcctat ttaagagaat
     2221 tggaggttaa aatatctgaa gaaagtacaa aaacaaactc tccaattata gaaaaaatca
     2281 tggatgaata ttcacataaa ctagaattat tttcagattt aaatggatat ggatataaat
     2341 ctgaagccaa aggagttcta aaaggattag gatttagtga caatgatatg gataagccaa
     2401 taagcatact ttctggtggt gaaaaaacta gagttctttt aggaaaatta cttcttaaga
     2461 aaccaactct attgttactt gatgaaccta ctaaccactt agactctgaa gcaatagaat
     2521 ggttagaagt atttttaaaa caatataaag gaactgtcat gttaatatct catgatagat
     2581 atttcttaga ccaatccgta aatagaattt ttgaagttca caacaaaaaa cttaaagtat
     2641 ataatggtaa ctactctaaa tttgttgagt tatctaaaat agaaaaagaa ctagagctta
     2701 agaaatttga agaccaacaa aaagaaataa aaaagcaaga agaatcaatt gaaagactaa
     2761 aagcttatgg tagagaaaaa cacctaaagc gtgcaagaag taaagaaaaa gcattagaca
     2821 aggtagacgt tttagataaa ccagaagctt ataggaaaaa agctcgtata caatttacgc
     2881 cttctgtcca gagtggtaat gatgttcttc aaattagaga tgtatctatg ggttatggag
     2941 aaagaatact ttttaaagac ttggatttag atatatatag aggggaaaaa gttgcactta
     3001 taggtgcaaa tggtgttggc aaatctacat tatttaaaat aataactaat gaactacaac
     3061 ctcttagtgg caatattaaa tttggaacta atgtacatgt ttcctacttc catcaggaac
     3121 aaaaaactct taatttagat aacacaataa ttgatgagat ttgggaaaat aatactcatc
     3181 ttactcaaac tactttgaga aatatgttag gggcattttt atttgtagat gaagaagttt
     3241 ttaagaagat ttctacattg agtggtggag aaagagctag agttgccata cttaaactta
     3301 tactatcaaa tgcaaactta ttgttattag atgagcctac taatcaccta gatattgatt
     3361 ctaaggaagt ccttgaagag gcattaacta attatgatgg gactatattt acaatatcac
     3421 atgatagata ctttctaaat actgttgttg ataaaatttt agttttagat gaaaatggaa
     3481 ttactgaata tctgggaaat tatgattatt atatagataa aaaaaggcaa atccaagaga
     3541 tgagtattat agaagaaaaa gaagaaaaaa ctagaactca aataaaagat gaaaaaagaa
     3601 aagagagaga acaaagagaa atagaaaaga aaaatagaat aaaaagacaa aatatagaaa
     3661 aagaaattga aaagttagag attgaaattg aaaaattaga tatactttta tgtcaagaag
     3721 aagtatactc taatccagat aaagctaaag aagttagcca agaaaaaatt aatttagaaa
     3781 ataaccttgc ctctctatat gatgagtggg aagaatttat gtaaaaagga gaggattatt
     3841 tgcaagaact attttcttta aaagacgaaa ataaaagatt ttataaaata cttctatctt
     3901 tatgtatacc tattatcata cagaacttaa tttctacttc tgtaaatgta atagatacaa
     3961 ttatgataag tagtttgggg gagacatctg tagcttcagt tggagttgca aaccaatttt
     4021 tctttctttt taatatgtcc ttatctggta tcacaggagg tgctggagtt tttatttcac
     4081 agttttatgg aaagaaggat gtgagtaata taagaaaagt cacaggtctt acttgtgtat
     4141 tggcaattgt tttaagtttt gtatttgtta ttcctgcttt actaacacca aagccaatta
     4201 tacacatatt ttcgtatgat tctgaggtgg taaaactttg tatagattac tttagtattg
     4261 cagtatttag ttatccatta attgctgtaa gtactgtatt tagtactggt tcaagaggtg
     4321 ttagaaatcc taagttaggt atgatttgta gtgcatttgc acttgtaaca aatgttattt
     4381 taaattatgg atttatattt ggtaacttcg gtcttcccgc attgggtgta aaaggtgctg
     4441 ctctagctac tgttattgct agaatatgtg aattaatttt aatgattact tatgtttacc
     4501 tttataaaaa agactatata ttaaagtttg gtctaaaaaa tctaaaagct attgataaaa
     4561 tttttataaa atctttttca tcaaaaagct ttcctatatt tgtaaatgac tctgtatggg
     4621 caattggaac tgtcctgtat tctgttgcat atgcaagagc tggtacttct gctatagcag
     4681 ctagccagat agcaaccagt acaggtaatt tcttcataat gacagctgta tgtattgcat
     4741 ctggtgcatc tattatgctt ggaaatgaac ttggagctga ccatattaaa agagccattg
     4801 aatatgctaa aaagttttct atacttgtat tttcagctgg tttaatactt ggaattattt
     4861 taattttaaa tataccatta ttattaaaaa tgtttagtgt ttctgatagc ttagcttctg
     4921 atataactaa gatattcttt atcatgggaa tactaatggc tcttaaatct tttaatacat
     4981 tggttataat tggtatttta agaagtggag gagatactaa atatgctcta tttctagaat
     5041 taggatgtat gtggttagct tctattcctc taacttttat agctgcattt aaaggtgcac
     5101 ctatatttgt actcgtttta ctcacttata gtgaagaagt ggttaagttt atatttggtg
     5161 ttcctagagc attatctaaa aaatgggcta ttaatatagt aaaggaaatt gattaataat
     5221 tatactatac attataagta tacaatattt ctaaacagtc tcattgaaaa taactaaagt
     5281 atttttgatt ttcagtgaga ctatttctat tttttacaga aattattata ctttattttg
     5341 attaagtaac aatttttact catcttccaa aaactctttt acttcaaatg ccaatttatg
     5401 taattcatca tagtttaaaa tttcatatgt attacccatt ttttttacta tctccttttc
     5461 agttaacttt agcagagaat ggtatctact ttttctacta attgaataga tttcactgaa
     5521 attacgaata gcttttatat tgcagacatt attttcttgt ttatttgata tacaataggc
     5581 aataacttct gtgcaagaaa atagttctct taccattctg gttttatata attcatatat
     5641 tcttcttgta cattttctgt gaaatagtgc aataaaatct ggtttgagat aaaactcttc
     5701 aaatacattt ttttcaaatc gaaataattt tatattggtc tttgcaaata tatcacattt
     5761 caaattatgt tcatatatgt aactgatttt gcctataaat tcattttcag gtacaatgtc
     5821 aacaagaaat ttatttcctc ctctagtagt acactccact aatgcaactc cctctattat
     5881 aaagaacact tcttgtaagt ctttatctga tgctacaaat ctttgaccct ttttatattc
     5941 aacaattgat attttgttta aattattttt aataattgga tatatacttg ttttttctgc
     6001 tttcatcaaa gttacctcct ttacaatata ttgtataaaa tattttattt atatgtttta
     6061 atttatatta tttttaataa aagatacaat tacaactaaa ttaaacttga caaattcatt
     6121 ctactcacgc agaataaact tatacatata tattttcata tataatttat tttaatataa
     6181 ttctatattt acaaaattat attatcaata gaatttatta aaataaattt ttttgggtgt
     6241 atattataat ttaatcgaat agaaatgaaa aatatacata cagtttaagt tttaagcaac
     6301 aattaggggg atttggtgac aagattttta attataagaa agaaagtgtc ttaatgcaac
     6361 ttttataaag tcattataga cactttcttt tttatttgat aaatttaaat tatcaaaatt
     6421 acattgcttt atacaactat tttgttggaa actatttatt atataaggag aaataaatga
     6481 aaaaatcagt agctctggta aatgatagta gaaaagattt aatagacttt ttagaaaata
     6541 atttgaaatt agtttttgga gattctataa atattaatag atacttcata aatgaaataa
     6601 atgataatga tattataaat gatgatgtga tattagtaat gtcagtagaa agattagaca
     6661 aaattataaa taatatacta gataagaaaa aagtgattgt ggtaagaaga acttttaggg
     6721 aagataaaat ttataatcta ctatctttac cacagggtac aaatgctcta atcgtaaatg
     6781 attctgatga aactactcta gaaactataa gtttatttta taaaattgga gtgactaata
     6841 ttagaccaat tccatatatg aatgataata actataaaaa tataaaaata gctataactc
     6901 caggagttcc agaaaaagta cctagcttta tctcagatat atttgattta ggtcacagat
     6961 atatagatat ttctacattt atagaaataa taaatttatt acaaattgat tcaaaggaaa
     7021 tacaatctaa tctagttaaa tactcagaag aaataataag tttagatact ggtataaaag
     7081 ataaatataa ggaattattt ttaaaaatag aagaattaga tacaatattg aatttatcaa
     7141 aagatggtat attatttact tcaaaagatg gtgaaataaa tacatataat agtaaagtaa
     7201 aagatatttt agacataaat gaggatatat atggaaaata cattgatgat atatttgtag
     7261 atagtttaaa agttttattg agtgagaaag agatactaga taaagttgtc gtattcaata
     7321 agaaatatat aaatgtaaat aaaaaaaaca tatacaatag ggatgaaaaa atgggcacat
     7381 attatagttt acaagaaata acatacataa aaaaattaga acaaaatctc actaaaaaat
     7441 taagagaaaa aggacaaata gcaaaatata catttaagga tataaaaaca aatagtccta
     7501 aaatgtttga atgtatagat ttagctaaga aggtttctaa atctgactta agcatactta
     7561 tcagaggtga aagtggaact ggtaaagaac tcatagctca gtctatacat aacaactcaa
     7621 atagaaaaaa tcaacctttt atagcagtaa attgtgcagc cgttcctgaa aatttattag
     7681 aaagccagct attcggatat gacaagggaa cttttactgg aggtctaaaa gatggaaaac
     7741 aaggattatt tgaacttgct aataatggaa caatattctt agatgaaata ggcgatatgc
     7801 ctttagaatt acaaacaaaa ctacttagag ttcttcaaga gaagcagata atgcctgttg
     7861 ggtctcacaa tgtaattaat atagatgtta gaatcatatc tgctacaaat aagaatttag
     7921 aacaaatgat tgataatagc caatttagag aggatttata ttatcgatta aatacaattc
     7981 caataaatat acctcctcta agagaaagaa aagaagacat attaattatt atggaagatt
     8041 taattaataa gaaattagta atcacaccag aagcaaaaaa acttatacaa aactatatgt
     8101 ggaaaggtaa tataagagaa ctccaaaatg ttacatccta tttaaacatt atgtgtgaag
     8161 atattgtatt agagaaagat ttacctccaa acttaaggtc ttcagataac aaaaatacat
     8221 cattaaaact aaaatatagt aagaatgata tattgaatat attagagata ctaattttaa
     8281 ataaggaatc agatgttggt attggtaggg gattaatttt aaaagcacta ctcgataaga
     8341 accttcaaat tacagaagga aaaattaaaa aaatatttga atatctaaaa aaagaagaac
     8401 ttataatttg tagttcagga agatatggaa gtaaaataac tcaaaaagga gaagattttt
     8461 acaataaatt aaaatataaa ggtctataaa attataattt atcaaacaaa gaaatctatt
     8521 caggattaaa gtactccttc aataggtttt tttatttttc aagtacccta aaattataat
     8581 ttattttgta cataaataat aggaattaat aggaatagga taattttttt atttcctatt
     8641 ttttttatat aaactagcat ataaaatata ttttcttcaa aataatattt tttactgaaa
     8701 attgaaacta tgcaatcatt ttttatacta tacatataga taaatgatta tatttattca
     8761 atattcttat aaaaaacaaa attctccatc gaaaattatg gcataataat tgcttattat
     8821 attaataaaa caataaaaag caattggagg aataaaaatg caacaggctt tgaaattaaa
     8881 atatcaaacc aaagaattag gaaaagattt tgtaatagaa agttgtaatt ctataaaacc
     8941 atggcttata aacataagaa gagaattaca taaaattcca gaattagctc ttgaagaaaa
     9001 tcttactaaa cagaaggtaa tttcttattt aaaagaaatc ggaattgatt atatggaatt
     9061 tacaaaacac aatggaatta tggcatatat tttaaaagaa agtgcagata aaacgatttg
     9121 tataagagca gatatggatg ctcttcctat tgaagaagaa aacaacatac cttataaatc
     9181 aatacattct ggaaaaatgc atgcttgtgg tcacgatgca catacaacca tgctacttgg
     9241 agcatgtaaa gttttacatt caataaaaga taaacttaat gtaaatgtaa aattcttatt
     9301 tcaaccagca gaagaaggtt ttggaggagc aaaattttta gttgaggatg ggtgtttaga
     9361 aaaccccaag gccgattata tattcggact tcatgttatg ccacatatag aaacaggcct
     9421 tatcgaaaca aaatatgata cattaaatgc tagtgtagac actataaaaa taagtatcaa
     9481 aggtaaaaga gctcatggtg catatcctga aaatggtatt gatgcaatag ttacagcttc
     9541 acaaattgtt acatcattac aaactataat aagtagaaac ttagaaccaa ataatgctgc
     9601 cgttttgact ataggtaaaa tatatggtgg tgatgcacac aatgtaatat gtgaagatgt
     9661 aaaactagaa ggcactttaa gaaccttaaa tagcaaaaca agaaacttta tgattgataa
     9721 aatagctaaa attgttggag acactgcatc tgcatttggt tgtgttggga ctttacatgt
     9781 aagtgatgaa aactatcctg ctgttataaa tgaaaaagaa ttagtagata cagttatatc
     9841 aagtactaaa gaattacttg gagaggaaaa atttatattg agaccaaacc cgtctttagg
     9901 tggtgaagac ttttcttttt acacagaaca ttgtaaagga gcattcttcc atttaggatg
     9961 taaaaatgag gaaaagggct taatttcacc tcttcatacc tctagcttca atatagatga
    10021 agattgttta ccaataggtg ttatgatgca tgtaatgaat actttgtatt ttaattagta
    10081 cagtaattaa taaaagaata ggagataaat taatatggaa aaagctaaga aattaggtct
    10141 atttagtatg atattattag ggattaatag cataataggt tcaggtatat tcttactacc
    10201 aggtaaagtt tatagtctag caggtcaaaa cagtatgttt atatacattt ttgctacact
    10261 cttagttcta tcaatacttt tatgttttgc agaagttgga agcatgtttg ataagaatgg
    10321 aggagcatat ctatactcca aaaaagcttt tggagatttt ataggatttg aagttggtac
    10381 aatgtcttgg tttattagaa ttatatcatg gtctactcta gcagtaggat ttgcaacagc
    10441 tttaggttca ttttggccag aatctgccac tgaatacaaa ggttgtatag ctgcaatact
    10501 cgtaacacta ctttcaataa atagcttatt tggaattaaa agcacaaaga taatgaataa
    10561 tgtaataact atagctaaat tagtaccatt aatagttttt gtgatagttg gaatattctt
    10621 tataaaattt gtaaatattg ttccttcagg aaatgtagtt aactctagta tgggtccagc
    10681 cattatatta gtattttacg catttaccgg ctttgaatca tttattgtgg caagtggaga
    10741 aatggaaaat cctaaaaaaa atcttccaat cgcattaata accacaatat ttatatgtgc
    10801 aataatttat atattaattc aaattgtctg tatgggtata cttggagata gactatttga
    10861 aaacagtatt ccaattgcag atgcttcaag tgtattttta ggcaattatg gtaaagtttt
    10921 catatctgta gcaacattaa tatctatatt tggtataaat ataggttcat caatagtaac
    10981 tcctaaatgt ggttcttctc tagcagaaga aggttctctt cctgcattta taggaaaaac
    11041 aaataaatat ggtgctccat atgtagcaat aataatatct ttaatttgtt gtataccact
    11101 tgtgctaact ggaagttttg aacaacttgc agtaatgagt gtaattgcaa gatttgctca
    11161 gtacatacca acttgtttat cagtaatagt tttaagaaaa agaacagatg ttaaggcctc
    11221 ttttaagatt ccatttggtc ctgttatacc aatggtagct attttaggta gcttatggtt
    11281 gttacaacaa gcttgggcag aagatatttc aaaacctata actcaaaata gagtcttaat
    11341 aggattaggg gcaatgttgt taatagctcc tctatatata tttatgaaaa aaaataagga
    11401 aatcgagcat aaaaataaat cagaagattc taaattatat atttcgagag aaaaaattca
    11461 ataatatttt atactattac ttctcataaa atataataaa tataaaaaaa ttgtagataa
    11521 aactatttta taaaatttat ctacaatttt tttatataaa catatatcaa aatcaaaata
    11581 ttattaattt atactttaaa gattcccatc tttatcatat tttattctta taagaaaata
    11641 taatcctatt actactaact ctatcactaa aaatactcca taggtaagaa ttgaatcaat
    11701 actatctaac ccatttctaa aaaaaattaa tgctatacct gctgctataa tccctatagc
    11761 attttttctt actgcatttt gcccaactcc ataaaaacct ggcatttttc cttttatttc
    11821 gacagcagtt aaaattatcc caaacactat aaataatact ccaataagca tcataatcat
    11881 actaatcatc taattacctc cttcacttaa ggaaatttca atttctaaaa tctaaaataa
    11941 ataatattta ttttttataa taatattgta tatccaatta actattttgg aaattagtac
    12001 caataaataa tgtttaaata ttgaaataat atattaattc taatcaatat tattaatata
    12061 tatacatttt tacaaatata tttaaaaact ttacttttaa ttcttactat ttaaataagt
    12121 ataatttatt ataataactt ttaattaatt tatactttta aattgtaaaa atcaccccta
    12181 atcactatat ttaccactat aataattgaa gaggtgattt atataacaga tactatttat
    12241 gcagttatta taaaatcaca ctcttaatta taatcaactg catattttta taataaaagg
    12301 aaataaagta agaaagataa atttaatcaa atatgctaaa tcaatttaat agtatactta
    12361 ttactgaaat atttacattc catcaggagt tctttgacca tgtatcaatt tattataata
    12421 tgtattatat gatcctcctc ttgcattata atcattattc ttgcagttac aagatgaaga
    12481 tgccatcatc ttacaagtat aaggacacat aatagtacaa acacctcttg tctcaacacc
    12541 tatattattc atttctattc tttttccaaa actcattcat atcctcccct tttctataag
    12601 atcacttact ttataccttt attaacctat accatattga ttatttttag aacattatca
    12661 aaactcgtaa attgcctatt caaaagtttt tcctctggaa tttttatatt aaagttttct
    12721 attacctcgc aaaatactaa aagtaattct cttggctgta tatgtaaaga cggtgaaaaa
    12781 aataattctt tttttaaatt gttattttta ttaaaattaa ttccagtcac atcaaaaaat
    12841 atattctcta gtagtaattc cttttcagtg taattcataa ttaacctcca attctaaata
    12901 gatattactt tagcatactc ttttaattga ttcactatat tgtccactaa ttctagtaat
    12961 tgtttattat catgtataaa aaatgttttt tcataatctt ctatatcttc aacaaaaaag
    13021 ttttcatcta atgacatata tgaaaatttt tgcttcacct ctgtctcata tgtatctata
    13081 acttttttct ctatactaaa ataatctata tttataccat gtcttccttt aataaaatat
    13141 ttaagttctt cacaatattc tctatcatat atacttgatg gaatacataa tataccacaa
    13201 tcatttatta ctgcattaga aatttcaaaa gctctaattc caaaattacc aaccattttt
    13261 ctagagaaag ggaataattc tcctggaact tctataatta ttaaatctgg ctttgcctct
    13321 acctctttat cttttataaa atggttaaaa ttaataatct ttgtcttact acttatcgaa
    13381 tcatcaaaca taaactctgg tataggaatc attttaaata aatagctctc ttttttagga
    13441 ccaaattgta atattgaaaa cccccttttt cttagttctt ctcctatcct tatatttaca
    13501 tcaaaagtat ctaaccctcc ataaagacca caaataaata ttattggtgt gtttatctgt
    13561 gatataccct ctatatctct tatttcatat ttttctaaat tccattcatt aatctttttg
    13621 aattcataat gagatatata cctagatatt ttattaagat catcacgtgt atataatatc
    13681 tttttacctt cttttagagc tacttttaat ttaggaatta taaatttttc taagtctaga
    13741 ttttcttttg aatcaacaaa ccataccaaa gaattctttt taagttcact ttgaaaatca
    13801 tttgtaactt tagtaataat attattaaca ttatactcct tattgcataa cccccagcca
    13861 gaaggactaa ccagtgaatt aatttctata tctgaaattt tattgcaatg atttaaaata
    13921 gtaaaaaaat tataattaaa tggatatata agtacttttt ccataatccc atctttcctt
    13981 tcattagaaa atttaatatt gatatcctag agatttcaaa acgcagtaat ctttcatatt
    14041 attttctgta gaatgtctaa ttctgtcaca atcatgtggt tttatttcat tttcattaca
    14101 tttagtatca tcaatcttag ctatacatat atcacaataa ctataggccc aacaatcaga
    14161 acattgctct tcatataatt ttcctatatt taaaagtttt tcagcctttt ctatatctac
    14221 tccactatct aaatttccaa tcatacccat tttagaactt tcacttactc tctcacatgg
    14281 aaaaaattct ccatctgtag ttacaaatat tctaaaagcc cctggtatac atggtccacc
    14341 atgatgccac acatctggta actctaatct accttcaata tttttctcat atctaaaatc
    14401 atctaataag gctctttcta ttcttgataa aggtgacata ctctcatctt ttataaattt
    14461 taatttgttg agtaaagtca agaatctatc ataactctta cctaatatat attcatctga
    14521 tgaaactatt tctttatcag aatagttatt attaagagtg gaagctaaaa agtttgaatc
    14581 ttttaaagtc tcatttttag atataaaatc atctatagaa atgtaatcat tttctgaatt
    14641 tatcactgta ttaaatgtaa tattcttttt ataatattct ggatatttat tttttatgta
    14701 acgtacattt tttaatacaa catcatgaga tccttctcct gtatttgcaa attttctact
    14761 tttatcatga atttcttttc ctccatctaa actaatcata gttgttatat tattttttac
    14821 aaagaattct attatttcct cattaaataa agttgcattt gtagttagat taaagtatat
    14881 ttcctttcct tctccaacta cttttacata ttctactatc tgcttaatca aatcaaactc
    14941 aagtaagggt tctcctccat aaaagccaac actaacttca tttaattgac tagaattctt
    15001 aatcaaatag tcaatacttc tttttgctac atctatattc attcttttat ttgtatgaac
    15061 tctgttgctg tatgaaccag aataaatgca atagtcacaa cgtaaattac aattttgggt
    15121 tacttgtaaa ataatagagg atagattatt ttctatatga tattttatat acttagtttc
    15181 agggtgcatt gttttattta cccttttatt ttttagaaat ccataattaa tcaaggtatt
    15241 aatttcgtct tctatttcta tgttccagtc actttctttt tctaaattat gctcaatttt
    15301 atttagataa ttataaacag cagcatttac ctttaaaatc ttatccgtat taacatcata
    15361 aataaaatat gattttccag ctttaaatgt gtgtataaat ggtttttgat tcattaatta
    15421 acctcccttt aatatttatt tgcagttaca aaataaatta aaataaacac cctttttgtc
    15481 cataagttca tcatgtcttc cactttctaa caactttcca ttttcaagaa ctacaatatt
    15541 gttagttatc ttttttgcat gttggatatg gtgcgttata aatattccaa tcttattttc
    15601 aaatgttttt ttatataaag aaaggagttc atttcccgct tcagaatcta aagatgaatc
    15661 tggttcatca aaaatgtaca tatcagcatc tttcataaga gttctagata ttgctacttt
    15721 ttgccattca cccatagaaa gttgcttctt accaaaccag gttcctaaaa tagtttctaa
    15781 tccatcttcc tcataaatac ttggtttcaa tttagtttct ttaagtttac ttttaacttt
    15841 ttcttccaag ttactcttat atacatctcc catggcgaca ttttctttta atgtagcctc
    15901 atatttaata taatcttgaa acatacaact tacttttcta atgtattcat ctttttcaat
    15961 atcctttaaa ttttttccat ttatcaagat gtcaccttca taatcatcat aaaaacctaa
    16021 taatattttt aaaagtgtcg ttttacctga accatttttt cctagtatag caataggatc
    16081 atttttattt aaagttaaat taatattttt taaagaatac tctgatgaat gattgtattt
    16141 atatgaaata tttattaact ctattttatt tatatcccct attttttcaa atatttttat
    16201 atgttcaatt gagtcttctt tacagttata atctagaaat ttaaacagta aatctgtata
    16261 aaaaagatct tgtattactg aactaaataa cgaaaatatt gattcaacat tatcctttat
    16321 agattgaact gcttgtatat atgtagtcgc atctcctatt aaaatcttct ttaaaatacc
    16381 ttgaattatt ataaatccta taattcctgc agatacaatt gaatctaaaa ctgtacataa
    16441 tagcgtagca ataaaattct ttttagctat attcatatct tgagatataa tatcctcttt
    16501 taatttttta taatgattaa taagtttaga accaattcta aataccataa tctctttgta
    16561 agcataacct tttgacaaca aataatctat gtaccaggcc tttctttctt tccaagttct
    16621 gtttacacta acatcatatt gttcctttcc taatttaatg gaatataatg atttcaatac
    16681 aggaataaca ataacaacta ttattagcca aagttgaaat tgtgataata caacaataag
    16741 agaaagtgta gatactgatt ctcttattat tgaaataagg ttttcagata gtatagtaat
    16801 attaaatcct ttttgagatt gagctctatt tatcatgtca taagtattat catcctcaaa
    16861 atcttttaaa ctcaaagtta aagatttctc taacatcatt gtttcaatat gtttttcaaa
    16921 atttttatta aactttattc tataatatcc ataaaaactt tgaaaaattg aaggaattat
    16981 atttatgatt aaatacataa ttaacaaaac taatatttct tttacacttt tcgaaaatgt
    17041 ttgcatatta ttaatgatat tttgacttac taacaaagaa atagatggaa caatcccctg
    17101 aaaaattgcc ataaacatag agataaataa atatctttta tcatgtagat aaatcatatt
    17161 aaaacaattt cttacacctt taaaaaattg aaatttacca ttttccacaa tacacctcca
    17221 tacttaataa aattgaaata aaatatttta ttaattaata taata
//
LOCUS       DAELSY010000081        16981 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.081, whole genome shotgun sequence.
ACCESSION   DAELSY010000081 DAELSY010000000
VERSION     DAELSY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16981
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.081"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            120..431
                     /locus_tag="KPG09_002368"
     CDS             120..431
                     /locus_tag="KPG09_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG0966972.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      524..616
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            716..1510
                     /gene="thiD"
                     /locus_tag="KPG09_002369"
     CDS             716..1510
                     /gene="thiD"
                     /locus_tag="KPG09_002369"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBG0966973.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1534..2322
                     /gene="thiM"
                     /locus_tag="KPG09_002370"
     CDS             1534..2322
                     /gene="thiM"
                     /locus_tag="KPG09_002370"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBG0966974.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGIYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            2345..2977
                     /gene="thiE"
                     /locus_tag="KPG09_002371"
     CDS             2345..2977
                     /gene="thiE"
                     /locus_tag="KPG09_002371"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG0966975.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITERYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEATCAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            3116..3418
                     /locus_tag="KPG09_002372"
     CDS             3116..3418
                     /locus_tag="KPG09_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0966976.1"
                     /translation="MIKILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRVPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3484..4233)
                     /locus_tag="KPG09_002373"
     CDS             complement(3484..4233)
                     /locus_tag="KPG09_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0966977.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(4227..5189)
                     /locus_tag="KPG09_002374"
     CDS             complement(4227..5189)
                     /locus_tag="KPG09_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0966978.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVKMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(5193..5651)
                     /locus_tag="KPG09_002375"
     CDS             complement(5193..5651)
                     /locus_tag="KPG09_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG0966979.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            6007..6363
                     /locus_tag="KPG09_002376"
     CDS             6007..6363
                     /locus_tag="KPG09_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0966980.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6435..7151
                     /locus_tag="KPG09_002377"
     CDS             6435..7151
                     /locus_tag="KPG09_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0966981.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            7144..7968
                     /locus_tag="KPG09_002378"
     CDS             7144..7968
                     /locus_tag="KPG09_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0966982.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQNILFFGMLGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIVLITMIYGFLISQTATLFICKYIFNFIFRNIPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIINLFRKYYNLIGFQFNNLVAGLLSFLIIMFILNGISYLLIKKKVSL"
     gene            7980..9179
                     /locus_tag="KPG09_002379"
     CDS             7980..9179
                     /locus_tag="KPG09_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966983.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYANILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQDIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDNKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            9188..10000
                     /locus_tag="KPG09_002380"
     CDS             9188..10000
                     /locus_tag="KPG09_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966984.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFIFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVLEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            10011..11279
                     /locus_tag="KPG09_002381"
     CDS             10011..11279
                     /locus_tag="KPG09_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966985.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNKNPKSRIRNDYLNIDKKYSDFVNDKG
                     KELIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     YQQNINEIKFSIGSKLYLEIVNNKSKNGDIEGHDSYSNLIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMIKNNKLNLIVTDMSNSIINLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGMKNSISGELKGFKGIVDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
     gene            11645..12826
                     /locus_tag="KPG09_002382"
     CDS             11645..12826
                     /locus_tag="KPG09_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0966986.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQVDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(12968..13885)
                     /locus_tag="KPG09_002383"
     CDS             complement(12968..13885)
                     /locus_tag="KPG09_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966987.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            14138..14932
                     /locus_tag="KPG09_002384"
     CDS             14138..14932
                     /locus_tag="KPG09_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0966988.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            14933..>16981
                     /locus_tag="KPG09_002385"
     CDS             14933..>16981
                     /locus_tag="KPG09_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0966989.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFACYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEELFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     S"
BASE COUNT         6684 a         1809 c         2726 g         5762 t
ORIGIN      
        1 gtatgtttta tacatatatt ataattaatt ctttgaagat tgcatattca aaatacacac
       61 catttttttt aattctataa tataatattt atatatttaa tgttaggatg tgatttaagg
      121 tgtattttac atatattata agatgtaaag atgattctct atacactgga tataccagta
      181 atatagttag aagaatgaat gaacataagt taggaattaa ttcaaaatac acaagagcaa
      241 agggatttga aaaactagaa gtttattttg tgaccaacac aaaaagcaat gctatgaagt
      301 tagaatacta tataaaaaaa ttaactagaa ataaaaaatt gtctataata aaaaacccaa
      361 gtatacttat aaatttaatt gataataaag aagattatat tattggtaag gaaatagaac
      421 aattaacata aatatttata gtaacattat aatgggtgca tatagtgtaa aaacttgaca
      481 ataaatacta ttgtaatata atgaagttat aaatatagta aattaaaata gctaggggag
      541 ccaagagctg agaggtatac cgacccttac acctgatctg gataatacca gcgtaggaaa
      601 gcttagtaaa aattcaagta tgtattattt gtgatatatg ctatttctaa gctataccta
      661 ttgtaggtat agctttttta attaagttat ttaaattaaa ctttaggagg caacaatgag
      721 taattataaa ataccaacac ttacaatagc tggctctgat tcatctggag gagctgggat
      781 tcaggctgat ttgaaaacgt ttagtgcaat tggtacttat ggtatgagtg taataacagc
      841 tataacagca cagaatacta aaggagtttt tgctgtagag gatttaaata agaaaataat
      901 aaaaaaacaa atagaggcag tctttgaaga tatacctcca agagctgtaa aaataggaat
      961 ggtttcaagt cctgagatta tacttgaaat agttgagaat ctaaagaaat acaatccaaa
     1021 atatttggtt gtggacccag taatgatatc taaaagtgga tattatctat taaagccaga
     1081 ggcaaaagaa aacttaatta agtatttgat accacttgca tacataatta caccaaatat
     1141 accagaggca gaagaaataa caggaataaa aatacataat gtagatgata tgaagagagt
     1201 aggagaagaa atactacaac ttggacctaa atttgtactt atgaaaggag gtcatttaga
     1261 tggagaagca gtggatattt tagttggtaa aaatatattt aaagtttata aaagtgaaag
     1321 aattgataaa aaaaatacac atggaacagg gtgcacatta tcttctgcaa taacatctta
     1381 tctagcatta ggatatgaaa taacagaagc agtaaatcta agtaaaatct atataacaga
     1441 agcaataaaa agaagttttg atataggtca tggagtagga ccagtacatc atttttataa
     1501 atttgagtaa taaaaaaata ttggaggtag aatatgtata atttaattaa agatgtaaaa
     1561 aaattaaatc cattggtaat tcattatact aataatgtaa ctataaatga ttgtgctaat
     1621 gtgactttag ctgtaggagc aagtcctctt atgagttttt catatgagga agtagaagaa
     1681 atggtaagtg tagcaaattc tgtagtaata aatataggaa ctatgaactc aaatatgttg
     1741 gatttatttc tattagctgg aaaagcagca aataagtaca ataaacctgt tgttttggac
     1801 ccagttggag tatttgcaag caaagcgaga gcagaactta caagtagact tttaaatgaa
     1861 gtcaaattta gtgttgttaa agggaatgta tcagaaataa agtttatagg tggctttaat
     1921 gtaaggggta aaggtgtaga ttcatttgat gaagaagaag attctactga gataataaga
     1981 aagattgctg aaaagcttga atgtgtagtt gtagcaactg gaaaaataga tataataact
     2041 aatggtaaag gtatatataa aataaacaat ggaactgata aattaaaagg aattacagga
     2101 actggatgta tgactgctag tttgattgca agttttatgg ctgttactga gaatatatta
     2161 gaagcagcaa caatgggtgt tcttacaatg tctttaagtg gggaattagc taatttaaat
     2221 aatccaccaa taggtacatt taaagaaaat cttatgaatg ctatttatca aatggatata
     2281 gatactttaa gtaaaaattc taatattgaa tttttaaatt aataaagagt aagggagact
     2341 taaaatgatt gataaagaat ctttaaaaaa atgcttaaag ttgtatttgg taactgactc
     2401 tgaaatgctt aaaggtagag acttttataa atgtcttgaa gatgcaatat cttctggaat
     2461 aacaactgta caacttagag aaaaaaatgc ttctggaaga gagtttttaa gaaaagctat
     2521 gaaacttaga gaaataactg aaagatatgg tgttaagttt ataataaatg atagagtgga
     2581 tatagcactt atttgtgatg cagatggagt acatgtagga cagagtgaca ttgatgtgag
     2641 agaggttaga aagcttatag gaaacaataa aattctagga gtatcagcaa gaactttaga
     2701 agaagctact tgtgcaaaaa atgatggcgc agactattta ggtgtagggt ctatattcac
     2761 aacatctact aaattagatg caaaatcagc ttcgtttgaa actgttaaag aaattaaaga
     2821 aaaagtagac atgccatttg tgctaattgg aggtataaat ttagacaata tagataaact
     2881 taaatgttta gaaagtgacg ggtatgctat aatatcagct atattaaaag ctgaagatat
     2941 ctctaaagaa gtggaaaagt ggacattaaa aatataaaat tggctctggt agtgaaaaaa
     3001 atggaaaaat ttgatataat ctaagagtaa aatttaagca attataaatg ataaactttt
     3061 ctacatatac tttttaatca agatatatgt agatttggtt aaaaaggagc tataaatgat
     3121 taagatattg ttagtgtgtg ttggaggcat gtcttctacc ctattggtaa ataaaatgga
     3181 gaaggatgca aaaaaaagaa atatagactg taaaatatgg gcagttggag aaggagatat
     3241 aaaatcggag cttgataact ttgatatttt acttcttgga ccacaactta gatttatgct
     3301 tgatgacgta aagtctatag ttggagatag agttccagta tctataatag atatggtaaa
     3361 ctatggtact tgtaatggtc atgctgtgtt aaattcggtt ttagaaatat taaaataaat
     3421 aataaaagtg gattctatat aatagagtcc atttttatta tttgttatta ataggcttaa
     3481 aacttagctt accatttctt tttttaatct ccacatagca agtgaaaaac ttgaacagca
     3541 caatattaaa aatagtagga taacaggcag tatatctcca ataagaatag tctcaccaaa
     3601 tattaagcta cgtaaagcgt taataacatg ggtaaatgga ttgagcatca ctgctacttt
     3661 taatcctcca gataaacttt ctatagggaa aagagctgaa cttaaaaaga aaataggaag
     3721 tacaattgca gtcataattg tttcataaac tatttcattt ggcaataata aacttataga
     3781 ataagctaga cttgataaaa agaaggctgt cataaatatc agtactatca taagtaggat
     3841 tccaccaatg ccagattcta tccttactga gaagaatata cttactatac acaaaatagt
     3901 tacttctatg aaggaaacta agatagcttc taataattga ccaaggacga ttgaatacct
     3961 actgataggt gcaattaaga ctctatagaa acttccactg ttcttcatta taaagtttat
     4021 aataccacca ctactacaag aagaaaacac aaccagtaca atgatacctg gtagtataaa
     4081 agcagtataa ttattgatat ttatattatt catagtttgg tttgcaatag aactataaag
     4141 cacaagccaa agaaaaggtt gtaatatagt aactacaaat gagattggat ttttaaaacg
     4201 ccacttcata ctacgccata agatatttaa cattcccatt cctcctttaa gcttttttta
     4261 gaacttgtaa gtgctaaaaa tacatcttcg agacttggct ctacaatttc tattgcatca
     4321 aattctatat catgttctaa aagccattta tttattaaag taaaagcagt tctactatcg
     4381 tttacactta taaaaattga attttctcgt acgctcataa actttactaa accagtactt
     4441 ttaattgaat ctttatattt ttttatatct tcagtattat ggaaagttat acgtagtata
     4501 ttttgtctaa tgtaactacg taaacttgat ggtgttcctt gtgctaaatc ctttccattc
     4561 ttcataatac aaatattatc actcaattgt tcagcttctt caagataatg agtagttaga
     4621 aatatagttg tgccgtactc atctctaatt tttaataaca tatcccacat agattttctt
     4681 gaatcgacat ccataccaac agttggttca tctaaaaata ggattttagg agatgatacc
     4741 atattcatag ctatgtccaa tctacgtttt acaccccctg aatatgatga agtaggatat
     4801 tttaagtaac tagataagtc aaacttatca attaaagagt ctattctttg ttttgcaatc
     4861 tgtggttcta ctttatacat tttactttga aataccatat tttccataag ggataaatgt
     4921 tcatcaatag agatttgctg tgctacacaa gcaatttgag tacgtatcca agaaggatta
     4981 tctactaaat cttttccaaa catttttaca tttccagatg taggtttgta aaaggtagtc
     5041 aaaatattta taagagaaga tttaccagca ccattaggac caagtaaaga aaaaatttct
     5101 ccagcattta cattaaagct tagaccattt aaagctttca caccattttt atattctttt
     5161 actaaattgt ctatttcaat cgctaacata tattattcct cctctttaag tggaaatatt
     5221 atctctgtaa tatagttatc tgggtttcct tttatcaaca tacctggtcc tttaataaat
     5281 acttctctcc aaggagtttg tatttttata ttattttcat gaatatattt aaatatcatt
     5341 tcatacgttt ttggcaaatt actataagag cctgtgtgag taaaaaataa agcttttgtt
     5401 ttaggaaatt cttttgtagt tatacccata cgatttggag tttcagcagt tggaacacat
     5461 aattccattt ctgcaacacc agttttttgg tcaacatcat agtagcaaaa gaatggtgca
     5521 ccgttagact ttccttttat agctttaaat acatttggaa aatactttgt tgcctcagtc
     5581 ataggtccat tataacgcat ggtagccact gtgactgatt cgatattttt gatttcaatt
     5641 ttgcaattca tttgtttacc tcctaatgtg tgttcatatt cttactagat ttttaagcat
     5701 taatacatat aagattttga ttagttgaaa acatataatc ttatctcgtt tgcttgtttt
     5761 caagtctaac ataacataaa atatatatcc tctcatttga tgctccattt tgtaaagcta
     5821 ttatttaagc tttactttag attttttagc aaattgaaat aaagcaattt ataaaataag
     5881 tatttacaaa ttaagtgtat taatcgtata atacacttaa taggatacta gataaagtct
     5941 gaaataaagg cgtatttttt taaagtaagt gtactatcgt attaatacac tagaatagga
     6001 ggaatcatga attttgaatt agataataca actcccattt atttgcaaat agtaaagtat
     6061 ataaagagac aaatagtaac aggagagttg aaaccaggag agacaatacc atcaagaaga
     6121 gaaatggcac ttaatctaaa agtaaactta aatacagttc aaagagcata taaggagatg
     6181 ggtgatatga atataataaa tacttttaaa aattatcaaa gcagtgtaac agttgatgaa
     6241 aatatattaa aaaatttaaa attagagctt ataaatgaat cactatctgt ttttatagaa
     6301 gatatgaaag caataaatgt tagcaaagaa gaagttttaa aaataataga agataagtat
     6361 tagtttttta acaaatagaa aacttagttt agaaagttta tttataagca tattggatgt
     6421 ggaggatagt aaatatgata gaaataaaga atgtcagcaa aacatataaa aggatgcaag
     6481 ggcttaaaat aagaaagata gaagctttaa aaaatgtaag ttttaatata gaaaagggaa
     6541 aaataactgc attacttggt ataaatggtg ttggtaaatc aactatgtta aaagcaatag
     6601 caggacttat aaacattgat agtggagaaa ttcgtataga tggagaaaaa ataaatgaaa
     6661 aggtatacaa taaattggct tttgttccag atgtacaatc tcactttagc aatacaacaa
     6721 taaaagaaac atttgaattt atggaaattt tttattcaaa gtggaataaa gaaaaatcaa
     6781 aagaaatgat ggacatattt aaattggatg aagatgaaat aattgataac ttgtcaaaag
     6841 gaaacattgc aagagtaaaa ttaatacttg gattttgcca agacccagag tacatacttc
     6901 ttgatgaacc atttacagga attgatttgt ttaagaggga agagtttata ggagtaatag
     6961 cgcaatatat ggaggaaaat caagccatta tcataactac tcatgagata gtagagatag
     7021 aaagtttagt tgatgaggtt gtaattcttg atgaagggca gattataact tcatttaatg
     7081 cagaagaatt aagagaaaga gaaggtaaat ctatattaga taagatgagg gaggtatata
     7141 agaatgaata aacttctata tctaataaat gtagatttga gaagaagcaa taagttttat
     7201 atagcttata tatcattttt ttcattaatt acacttagtt taaatttatt cgaaattaat
     7261 aaatttaaaa attctaaatt tattatgaac ttagcaatag aagattttgg aggtatattt
     7321 tatggactag ggatacttag taatcttggt tttatccaaa atatattgtt ctttggaatg
     7381 ttgggacttt ttatatacta tatttttatg tggaaaagag agttcttttc aaataatcaa
     7441 agtatttata cacttatgat gttacctcaa aataagttta agatttgtat atctaaatca
     7501 atagttttga taacaatgat atatggtttt ttgataagtc agacagcaac attatttata
     7561 tgtaaatata tatttaattt catttttaga aatattccta taatcaatat gaactttgcc
     7621 aaagatttat attatttaaa cttgaagagt ataccaattg attttatctc ttttatggct
     7681 atttatgtat ttttactact tatagctatt tctattgtaa attgctgtat attatttatc
     7741 ttttcctata taaataaatt ttatgtaatt ttaatcactt tgctgattat agcaggctta
     7801 tatttttcaa caatatatat aaatataatc aatttattta gaaaatatta taatcttata
     7861 ggatttcaat ttaacaattt agtagcaggg ttattaagtt ttttaattat aatgtttatt
     7921 ctaaatggaa tatcttattt gctaattaaa aagaaagtat ctctttaagg aggggtggta
     7981 tgaaattaaa atctaaaata tctatactta ttatattttt gactttagca atatatttta
     8041 gtataggttt attaaataat gaagataatt taaagataaa aactataaaa ggtgactcta
     8101 atgaattaga ccatataaac ataaattttg gatatgcttt gaatggatat ataaattcat
     8161 attatacaat aaatggaagt aacaaaacta aaaagactga attaataaaa ctacaaaagg
     8221 atagtaagaa cacaaataat ttaattgatg tatctagtgg ttcagattta attgctcatg
     8281 ttggatttga aatgaatgag gagtcaaaag atataaactt tgtgatagat gagaaaaaag
     8341 aagatttaga aaaaggtgta tttccatcag aaggtagtga taaagaaatt gaatatgaaa
     8401 aattagatat gaataatttt acaaggacaa gatataaagt agataaaagt acagatgatt
     8461 attatgcaaa tatactagca tcaaaaagat ataaaaaata cttatatgta tctgtacttt
     8521 atgctacaag tgattcatca gaaaataaca aagaatattc aagtactata gatataataa
     8581 aaattaatcc agaaaataaa aaaatcaaga ctataaaaag tataaattta agtaattatt
     8641 tttccgagaa ataccaagac atagagaaca taggtgtaag ttatgctagc aaatatgagg
     8701 ataagatgta tattttagta aatgtactaa atacaaacat aggtgataat agtaattcta
     8761 aatttcattt acttgagtat aatcttgaag aaaataagga aagcataatg gaattaaaaa
     8821 ctgatagcaa aatggcttta gcaaaagcaa agttagaagc taataaatta tatatggtaa
     8881 tgtcgaatga ttatgataat agttcaatta cagagttaaa tttacttacc tataatatat
     8941 tagataaaag cttagatgaa gataaatatg ttttagataa tagagaaaaa agaggaatta
     9001 gaagtattga aattgattca aataaaatcg ttgcatatac atcaaggcta aactctatat
     9061 tatcttggga taataaattt atgctatata taataaataa aaattctaag gaaattgtat
     9121 atgaaggaga attacaattt aacagttcaa atagtatgtt agaatttgaa ataaagtagg
     9181 tgagttgatg aaagatttaa taagattatt cgaatttgaa ttaaaaagaa atataaagaa
     9241 ttatatattt ataataattt gttgttgttc ttttgtaatt ttaaacatag taaaaaattt
     9301 aaatgactat aattacataa tagaaaatgc agttaaaagt gaacagttaa caaaaatcgg
     9361 aaatacgata gaaacggtaa atgttacagg tttctcaagc tttagaaata taatgggttc
     9421 tacagaatcg tggtttatat ttggtataat tgcatgtatt tgctatgctt tttttatttg
     9481 gtatagagac tttaatggta ggagtaaatc tatatataca cttataatgc tacctaaaaa
     9541 tagaatcaat atttatatat ctaaattatt aaatatatta tttctagtgt atagctatac
     9601 agtagtactt acaatatctt tgtttattgc aagtaagttg ttgcctagac atatgcttgg
     9661 taatgtcaca aactatggtt ttgtacaaga aacaatatat gagttaaaaa tgttacttcc
     9721 atatagcttt gaaatactat ttgtagagta tatattttta ttgataggat ttgtatctgt
     9781 agtatttaca tcaattctta taaataaatc tatttataaa gtaagcactt taataagttt
     9841 tctattttta gttttggaaa ttttagtgtt tataatttct atagaattta ttatacctta
     9901 tgaatacagt gatgtattta ctgttctata ttcaagtata aacatatttg tatgtagtct
     9961 tatatctaat aaacttttga agcaaaagat tgacttttag gagggatatt atgaaagcaa
    10021 aaaatatagg aaaatataca atatgtttta taatagtatg ttttttaatg attactatga
    10081 ccttaatatt gagttatttt aataaggata aactatctgt aaaaacttta aaaggcaatg
    10141 taaaagatat agggagagta agtgtagtat atacacctaa tataagcaaa ttttcttatg
    10201 aggaggtaat tataagtaaa aatggagtaa gaaataataa aaaccccaaa tcaagaatta
    10261 gaaatgatta tttaaatatt gacaaaaaat acagtgattt tgttaatgat aaaggaaaag
    10321 aacttatata taaagatgat ttgaatatag gagaagcgta tacctctgtt aagtctgatt
    10381 tagtacaagg aatgaatgct ttaaatgatt gtataaaggt ttcaaataaa aatttaaaaa
    10441 gtggggtaga agaaaatttt gatattaatt taaataagtc tgattttaaa gaaaacagtt
    10501 ggaaaacctt gtttaataaa atatataaag gcaatttata tatggtaatt agtgaaacta
    10561 atatttcagg ttctaaggtc aataatattt gtatattaaa gatagatata aaaaataagt
    10621 cttataaatt attaaataga tttaatttag gattacagaa tggatatcaa caaaatatca
    10681 atgaaattaa attttctatt ggaagtaaat tatacttaga aatagtaaat aataaaagta
    10741 aaaatggaga tattgaagga catgatagtt actctaacct tattatatat gatatagaag
    10801 ataacacttt taatataagt aataaattta tggataatct aaacaaagaa aagaatgcaa
    10861 atccaaagga gattggctta ttaagtaaaa ctaagctaga ttatatgata aaaaataata
    10921 aattgaatct tatagttacg gatatgagta attccataat caatcttgtt tatgatatta
    10981 attctaatga gattaaatta gaagattatg agatacttga tttaaaagta gattataatg
    11041 aagcatcatc tggagttagg aaagttaagt tactggatga taagatttat tctatttcag
    11101 gtatgaagaa cagtatatca ggagaattaa aaggatttaa agggattgtt gatgtagtcg
    11161 gaactaagcc agtaaaattt caagtatttg atatgaaact taaaaagaat atttatgaag
    11221 ctgagctaat aaatggagat ataagtttac aagaaaagtt attttttgtt gcaaattaag
    11281 tattagaatt aattttagaa ctttaaactt ttatatgtat cacagataag tataagtaag
    11341 ggagtgtctc aaaataaact ttttagttca tgagacactt tttttatatg aaatcaaata
    11401 tattttcatt gtttagattt attttgataa tgaaaaaaag attatcttta ttttaagaaa
    11461 gacatttatt tataattata tacaaaaata ttttttaatg tttttgtgta taattataac
    11521 aatttaattt atattaatgc acttaaattt gaaatagaat gcttatagtg gtaaaattaa
    11581 ttattagaat attagaaata ataaataaat aatacattgt acttaaaagg gggagaaatt
    11641 taagatgaac aaaaaatata ttaaagcagg gaatttaatt gatggaacag gaaatagtat
    11701 tatgcaaaat aagggtataa ttattgatgg agatactata gttgaaattg aagatataag
    11761 agatgattta aatggatatg atgtgtacga ttacttagat aaaactgtta tgccaggcat
    11821 tatgaattgt catgttcact tgacaatgga gccagttgga aatccaaaga tatattatga
    11881 taatgtatca gatgtagagc ttgtagtaaa tacgataaaa caagttgatg cttatttgga
    11941 ttcaggagta acatatatta gaagcttagg ttgtcccaaa tatgtggatg tacaacttaa
    12001 aaatcttata gacaaagggt ctatagatgg tcctggtata gttacttcag gtccagttat
    12061 atgtatgaca ggagggcatg gatattattt tggtattgaa agtgatggag tggatgaatg
    12121 tagaaaatca gccagaacag ttttaaaaaa tggagtagat tgtgtaaaga taatggcaac
    12181 tggaggtgta acaactgatg gagttgagcc agggtcacca caattaactt tggaggaaat
    12241 gaaggctgct gtagatgaag ctataaaagc aggtaaaata actgccacac atgcacaagg
    12301 aagaactggt ataaaaaatg cagttcttgc aggaattact tctatagaac atggtgttta
    12361 tttagatgat gaaataattg atttgatgct tgaaagagga acttacttag ttccaactgt
    12421 ggctgcacca tattttattc taaatgcagg aaaagaaagt ggaataactg aagctactct
    12481 tagaaagtgt gagattgttg ccattccaca taaagaatca ttcctaaaag catataaaaa
    12541 aggaattaaa attgcagttg gttctgatgc aggaacttct tataatgaac atggaaaaac
    12601 atattatgaa atgaagctaa tgtcagatta tggtatggat aatatggata taatagttgc
    12661 tgctacaaaa acagcatctg aacttttaag aattgataaa aattatggaa ctctagaaaa
    12721 aggaaagaaa gcagatgtta tagttgtaaa tggaaatcca gtagagaata tagacgtact
    12781 tgcagatgtg ttagcagtat ttaaacttgg taaaaaagtt agataaatag tataaaaata
    12841 aaaaagagtg tctaaagaaa tttttaaata attatagaca ctctttttta ttagtaataa
    12901 ttaaatcttt tatactttag aaaatagatt ctattttata tttagatata actctttaaa
    12961 atcattctta tgcaatataa tctatagaat ctacacatac aaagtatatt cttgaatcat
    13021 tagaatttcc aagtactata acatcatttt tcttacctag aacttcaaca ccagttagaa
    13081 ccaaactacc agctactaca gtaacttgat tatttatatt tgacatataa agattatcta
    13141 ggataccttt attacaacag caatcatcac aatcgcagtt acaattacat tctttgtaat
    13201 gttttttatc aagatagtaa cctaaagatg ctatgaaatc ttcataatct gcaggtgtaa
    13261 tatcaaatgc aacagcttta agattacaca atgatgctaa atctacgttt gctaatggag
    13321 tagatgcagc aagtataaaa tcaattatag cagcaagtat tgcatctaat atagccccaa
    13381 ggtctatcgt aggaggaata actgcatcaa taagtgcaat taattcagct actcctggta
    13441 tagttcctat agtattaatt aatccctcaa gagttaaagg gactggaata ggataagata
    13501 ctctaccagc tatatctatt aaatcacagt tacaagcaga aaatctttca aaagtaccat
    13561 caagtccaga taaattatct tttggaggag ctgaaagatc tatacctacc aaattagcac
    13621 ctactataaa gaaatctgag ataaaagcaa attgattaaa gtttacaaaa ggtcttagag
    13681 catcatatct tagaagttct aaggcctctc tcatactttt tttacaacaa cattgtttta
    13741 ttttaggttc tttatattta tttggataat tatagtatct atcagctact tgataatttt
    13801 catagtatct ttcatttgta tttggataat cttcctcata ttcttgtgta aatctaaagt
    13861 cctctctaca tttattattt tccactaaat acactccttt tatattttaa tttaaatgta
    13921 tagataatta agcttatcta tatagtaata ttatatttat aatgtaaaaa aatgttacaa
    13981 aagatattat aaacgaataa aaaataataa tttgtcagat aaacttgtaa atttttatga
    14041 ataatccatt ctaaaacaaa aaactgtgta taaaaaaggt ttatatgtta tatttatata
    14101 aatataacat atgaacgaga aaggagtggt ttgataaatg attgaggtag ataaattgtg
    14161 taagtctttt acaagggttg taaaagatga taagaataaa agttcaataa gaaagctaaa
    14221 gaaagtaaaa actaaaaaag aagaattttt tgctgtaaat aatgtttcat ttaaagttat
    14281 ggaaggtgaa attgttggaa tattaggtcc aaatggtgca ggaaagacta cattacttag
    14341 aatgttaggt ggcatattaa caccaacatc aggaagtata aatatttctg gatatgatta
    14401 ttcaatagat agaaattcag caaaaaaaga aataggttat ctctctggaa atacaaaact
    14461 atatggaagg ctttctccga gagaacttct aacaactttt gcaagtctat atgagatgtc
    14521 aaaagaagat atagaagaat caattgaaaa tattgtaaaa ataatggata tgagtgaatt
    14581 tatagataat agaattgaaa atctatcaac tggtcagact caaagaacat ctatagcccg
    14641 ttgtttaata cattctccaa aagtatatat ttttgatgag ccaacactag gtctggatgt
    14701 tttaagtagt gcatctatta tagattttat gaagaatgaa aaactaagag gaaaaactgt
    14761 tctttactca actcattata tggaagaagc agaaacttta tgtgacaaga tttttatgat
    14821 tcacaatggg aaaataattg catcaggaac accacagagt ttaaaagagg aatctggagt
    14881 aaataatttg agagatgtat tcataaaact tgccagaaag gaggacattt aaatgagaag
    14941 taagattgta aaatacatat ttaaaaaaga aatgttagac atattgagag ataaaaagac
    15001 tttatttatg atgattgttg tgccacttct tttatatcct ttacttatgt tattaatgat
    15061 tcaagtaatg aatatgtctg caagtagtat gaatagtaag gacattaata tagcttttaa
    15121 taataaacct aataaggtgt tagtatccat gattgaagat gataatttag aaaaagatac
    15181 taaaaatact tcaaatgata aaggcaaaat aagagtgtta gatgtagata actataaaaa
    15241 gtcattagaa gaaaataaaa ttgatgctta tataaagatg gaagaaaaaa aatcatctat
    15301 aaattatcaa atatatataa attcttctca agaaaattct tctaatgcac taaaaagaat
    15361 tgaaactgtt ttggataaat ataaaagatt taccatagaa aaaactttat ctgaaaaagg
    15421 attagatgta caagctactt tagaaccaat aacatatagc actgtagatg ttgcaaagac
    15481 ggaagaagtg gcaggatact ttttaggtca gatacttcca tttatattaa taataggagt
    15541 tttactaggt tcaatatatc cagcaataga tgttatggct ggagaaaagg aaagaggtac
    15601 attagaaaca ttatttacac tcccaatatc aaacttagaa ctggttatgg gaaaatatat
    15661 ggcagtttct cttagtgcaa tagtaacagc aattttaaac attttatcta tgggactgac
    15721 tatggcattt attttagtta gtggaggtat tagtagccaa tttggctttg gaaactttaa
    15781 ttatggagac ttagtcttac caattataac tactttaatt tgcatatgtc ttttttcaat
    15841 ggtagtttca gcaataagta tgtgtgtatg ttcattagca aaaagtttta aagatgctca
    15901 aaactacata acacctctta tgttaattat tatgatacca tcatatgttt ctatgatacc
    15961 aaatatagta ttggatagag tgacagctgt aatacctgta gtaaatatat ctttacttat
    16021 aaaaagtgta ttatctttta aaagcgattt aaatttaata gccttagttt tagcatcaaa
    16081 tgtagccttt gttatattgg caatcatact actgtctaag atgttcaatt ctgaggaaat
    16141 actttttggt aatagtaaga gtttttcttt cttagagaaa agaagtaata taaaaaaagg
    16201 gtctatacca actgtaagtg atggtgttat tttatatgca gtaggattag ttctattgat
    16261 ttatgttggt tctttagttc aaattaaatt tggtatggca ggtattataa tgacacaatt
    16321 catgataatt tctttaccat tattatttgc atgctatatt aaagctgatt ttaaagaggt
    16381 atttagcttg acagttccta aattaaagca tgtgtttggg agcattgttc tttggattgg
    16441 tggatacata cttattatga ttataacaca gctaatttta ttcttatttc ctcaaaatat
    16501 ggaagttgct gaaagtttaa ataaggctct ttttatcaaa gatagtgttt tactgaattt
    16561 actagcaata gctttattac cagctatatg tgaagaactg ttttttaggg gatttatatt
    16621 ttcttcattt agtaaatcaa aagataaaaa taagtctatc aaactcgcta taatatgtag
    16681 tggggtacta tttggtatta tgcatatgga ttttattaga ataattccaa catcaatatt
    16741 aggtattata tttgcataca gtgtgtataa atctggttca atatttgtat caatgttatt
    16801 gcatttttta aataatagtg tagcagttgt gttaaatcat tatacaactg gaaatataac
    16861 caacttatat aaatttgttg aagttgattt ttcgaactta aatgtactta aatttgcctt
    16921 aattcttcta ataagtctta tattaattat gttaggatta agagttttag atagaaagtc
    16981 t
//
LOCUS       DAELSY010000082        16949 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.082, whole genome shotgun sequence.
ACCESSION   DAELSY010000082 DAELSY010000000
VERSION     DAELSY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16949
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.082"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..360)
                     /locus_tag="KPG09_002386"
     CDS             complement(<1..360)
                     /locus_tag="KPG09_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0966990.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKE"
     gene            653..870
                     /locus_tag="KPG09_002387"
                     /pseudo
     CDS             653..870
                     /locus_tag="KPG09_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            928..1290
                     /locus_tag="KPG09_002388"
     CDS             928..1290
                     /locus_tag="KPG09_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966991.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            1274..1990
                     /locus_tag="KPG09_002389"
     CDS             1274..1990
                     /locus_tag="KPG09_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966992.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVVEFTEEDEEYEEELKQNIIE
                     NIKKNFDEKKLVQVYKYRGV"
     gene            2010..2429
                     /locus_tag="KPG09_002390"
     CDS             2010..2429
                     /locus_tag="KPG09_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966993.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            2419..2943
                     /locus_tag="KPG09_002391"
     CDS             2419..2943
                     /locus_tag="KPG09_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0966994.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYVDENNNEESISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVLITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            3255..3812
                     /gene="efp"
                     /locus_tag="KPG09_002392"
     CDS             3255..3812
                     /gene="efp"
                     /locus_tag="KPG09_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBG0966995.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            3904..4359
                     /locus_tag="KPG09_002393"
     CDS             3904..4359
                     /locus_tag="KPG09_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0966996.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCPSCDDRE"
     gene            4522..5232
                     /locus_tag="KPG09_002394"
     CDS             4522..5232
                     /locus_tag="KPG09_002394"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBG0966997.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            5286..6356
                     /locus_tag="KPG09_002395"
     CDS             5286..6356
                     /locus_tag="KPG09_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0966998.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            6384..9938
                     /gene="smc"
                     /locus_tag="KPG09_002396"
     CDS             6384..9938
                     /gene="smc"
                     /locus_tag="KPG09_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBG0966999.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKILEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEASKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKVSLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEAYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSIATEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIGSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            9965..11245
                     /gene="ftsY"
                     /locus_tag="KPG09_002397"
     CDS             9965..11245
                     /gene="ftsY"
                     /locus_tag="KPG09_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBG0967000.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDSLENLEETIFSG
                     LEEEVIDKVEDIEEKTEESVNNEGTEEIENFEKIEVEMDSNAKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            11348..11716
                     /locus_tag="KPG09_002398"
     CDS             11348..11716
                     /locus_tag="KPG09_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBG0967001.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            11713..13065
                     /gene="ffh"
                     /locus_tag="KPG09_002399"
     CDS             11713..13065
                     /gene="ffh"
                     /locus_tag="KPG09_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBG0967002.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMAELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            13103..13375
                     /gene="rpsP"
                     /locus_tag="KPG09_002400"
     CDS             13103..13375
                     /gene="rpsP"
                     /locus_tag="KPG09_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBG0967003.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            13440..13667
                     /locus_tag="KPG09_002401"
     CDS             13440..13667
                     /locus_tag="KPG09_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBG0967004.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            13775..14290
                     /gene="rimM"
                     /locus_tag="KPG09_002402"
     CDS             13775..14290
                     /gene="rimM"
                     /locus_tag="KPG09_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBG0967005.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            14299..14994
                     /gene="trmD"
                     /locus_tag="KPG09_002403"
     CDS             14299..14994
                     /gene="trmD"
                     /locus_tag="KPG09_002403"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBG0967006.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVYIYNIRDFSNN
                     KHKKVDDYPFGGGVGMVMTPQPIYDTYKHIITTHNISNPSVIYLTPKGKVYNQSIAKQ
                     MSLKEDIILLCGHYEGVDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            15179..15529
                     /gene="rplS"
                     /locus_tag="KPG09_002404"
     CDS             15179..15529
                     /gene="rplS"
                     /locus_tag="KPG09_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBG0967007.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            15850..16746
                     /gene="ylqF"
                     /locus_tag="KPG09_002405"
     CDS             15850..16746
                     /gene="ylqF"
                     /locus_tag="KPG09_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBG0967008.1"
                     /translation="MSNEYEEYLMDDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINNIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIAATV
                     LNEFRDGKIGNITLEVPENVKR"
BASE COUNT         7302 a         1533 c         3148 g         4966 t
ORIGIN      
        1 ttctttatct gttccgatgg ggtcttgtag agatacttga gttttatttt ttttgttaga
       61 tctaatattc attaaaatct cattttcaat acatctagaa gcatatgttg ctaatcgagt
      121 tcccttatct atgttataag tttctatagc tttaattaat cctattgtac ctatggatat
      181 taaatcatcc tgatcttccg ttgaattatt atatttcttt gctatataag ctactaatct
      241 catgtttctt tcaattaatg tatcttttgc acttttatcg ttttctattt taaatttagt
      301 taaatactca atctcttctt caggggttaa gggtttttca aaagatttaa gagctgccaa
      361 taggtcaccc ccttttcaac ggtcacttta aattatatga aggagactta tccaagttga
      421 ctgttatcta ataaaataat atgcatattt ttagaattta aaacatatat ttgcaaactt
      481 ttgaatggaa tatgacataa agagtgggaa tttaaattta agtttttgtt gtatatatat
      541 gaaacaaggc gaagaaatta ttgatttata aagtataaat tggaattgag gtgtcaataa
      601 atataaaagg aaatagtatt tatatatgtt ttggtgttga ggtaaaagat taatgcaaag
      661 cattagagtc tataaaaata tgaatatagg aggtgtaatt ttacaatact taaaaaagta
      721 aatgtaatta attgtttgta taaagttgat tacaagcaaa aagtatcagt catagttaat
      781 tctgttaagg atgaaggtgg tgtgttaatt tatatatgta tgggaagaaa ttttgattac
      841 aatgaaaaaa cttacaaaag atatttatga aaaaacttat agttaaatgt ttatatataa
      901 aatattattt aggggttgtt tttattattg aaaataagaa agagtggttt tatatcaatc
      961 gaatgtataa taagtattgc tatattatat gtggctgttt atttagtttc tacatcattg
     1021 tataattgtt atagttttat cagtagaaat atatctgaca gagaaatgtt aagtacagca
     1081 aaaaaatata tagaagatga gaagtataga atacaaaata gtaagtatga gttaattgaa
     1141 gataagatag aaaaaaatta cataaatgga tatgaaatta acagtagaat agagcaaatt
     1201 ttagattatt atcaatgcta tgaaataaat atagagataa aaaatgaatt taaaaaactg
     1261 aggtttaata gctatgttac tagaaaataa aggctctata ttaataataa cattgataat
     1321 attttctatt ataagtactg tgtgcattat gtgtataggt ttaatttata cacataataa
     1381 tatgtttgag ttaggataca aggacattca actaaaagaa atttctttaa gtggaataga
     1441 aatatcagta agtaatgtac tttcttgtgt tgaggaagct attgatgtat ctaataatga
     1501 agacgaattt agagaatatt tcttaggaaa taatatgatt gaatgtataa aaaaaataaa
     1561 agatacatct tatagtagtt tagaagaact ttctttaaaa gtagaaaata atactattta
     1621 tgacagagga gatttttttg aatttgatat agaaagtaat gttagagaaa aagaattttt
     1681 aaaagcaata aaagtaagag tgaaaatcaa aaatccatgg ataggaattg acattgaaga
     1741 ttatatggat aaatctaata acaataaaat tgatgacaaa acattcaata attataaagc
     1801 atctgaaaat aatgaaattc taaatcaatt taaaaatgaa agtgaaaata taaggaatca
     1861 agaggttgtt gaatttacag aagaagatga agaatatgaa gaagaactta aacaaaacat
     1921 aattgaaaat ataaagaaga attttgatga aaaaaaacta gtacaggtat ataagtatag
     1981 gggtgtataa ctttgaaaaa agaaggaatg tgactgtaat agaattaata gtatctatca
     2041 gtataatact cttgatagtt acattatctt ttccaaaaga taatcttgaa aatcataaaa
     2101 taaatttatt tgcacgtcaa ctttgttcag atataaggta tgttaggcga attaatatgt
     2161 ttagcaacta caatacatat atatattata taaatcaagg taaacacgaa ggttatgtat
     2221 tgagaaaaga tggtaaaaac attaaatcaa taatgctacc taaaaatgca aaaatagcta
     2281 gaagtattga aataataaag tttaaatctg atgggtcgcc tcaaaaggcg tctacaatag
     2341 agatatataa taatagatta agaaaaacta taacaataac acctgtaagt ggaagagtac
     2401 ttctgaaaga gggaaaatat gaaacataaa tatggttact tgcttttgga aagtgtagta
     2461 tcattgtctt caatggtgat aattatacta gttttatatt ctatattttt gtctacaata
     2521 aacttaaagt taaaagtaga agataagata gaactacaac aacaaagtct tgaaatcata
     2581 aaatctatgg aaggaattat tagcaattct atgggaataa tgaatgtatc taattatgaa
     2641 gaaacattta aaaagacaac gtctataaaa tgtagatatg tagatgaaaa taataatgaa
     2701 gaaagcataa gtaataaaga aattatattg aatgaaagac gaaataagtt atttgtaaat
     2761 tctttaaatg gcgaaagtag tcaagcaggt ggttatgaaa ttggagatta tgtagatgag
     2821 atgtatgtat taattactaa taatggtcaa tatgttaata ttaaattgaa attatcaaaa
     2881 cgtagtcaaa aatatgagac tgatttcaaa ataaaagtat ggaattttag tgaaagtata
     2941 taaaaataca taaatttaag ataaagaaat gacatcaata agtcatataa aatataatta
     3001 ttgagaacta tataactaaa taatatcaat atgtaaagtg tatagattaa gaatatagag
     3061 gataataata aaaatataga ataagtaatt tgccaaatac ttgtttatat tgtataactt
     3121 atgatattat gatattagta aaaatttgta atcctgactt gacagaattc aagtaagaga
     3181 tataatttta aaggcatagt aatgtaaaat ttactaaaaa ataagaaatt tattttaata
     3241 tggaggaaca aaaaatggta tcagcaggtg attttagaaa aggtgttaca tttgaaaaag
     3301 atggacaacc atgtttagta gttgattttc aacatgttaa gccaggtaaa ggagctgctt
     3361 tcgttagaac taaatacaaa aacttaaaaa caggagctat aagagaagaa agtttcaatc
     3421 caagtgaaaa attccctaag gctgttatag atacaagaca aatgcaatat ctatacaatg
     3481 atggtgagtt atattatttt atggatcaag aaaattttga acaaatacca ttaaactatg
     3541 agcaagttga agatgcaatt aagttcttaa aagaaaatga agttgctaca ataagatttt
     3601 accaaggaca accattccaa gtggaagcgc caaactttgc agagctagaa gttacagata
     3661 cagagccagg tataaaaggt gatacagcaa gtaatgtaac taaagcagct acagttgaaa
     3721 ctggagcagt tgttcaagtt ccattattta taaatactgg agataaagta aagatagata
     3781 ctagaacagg tgaatattta tcaagagtat aaaaatttat gtatataaaa gcttatatgt
     3841 cttaataata taagatatat gagcttttag tttatatata aaaaagaaag cgaggggtgt
     3901 tacatgaaac atttatatga agaagttgca tacttaaaag gcctagcaga aggactagaa
     3961 ataagtgctg aaagtaaaga agggaaaatg atacataaaa tagttgatgc attagaagta
     4021 tttgcagatg ctattgtaac attggatgaa gaacaagaag aacttcaaga ttttgttgaa
     4081 tctatagatg aagatttagc agatttagaa gaagatattg ctgacatgga agaagatctt
     4141 tatgaagaag atgacgatga ggatgatgag gacttcagct atatagaaat ggaatgccca
     4201 aattgtggag agttagtaga aatagacgaa gatttgttat atgatgatga agtagatgtt
     4261 gtttgtcctg attgtaaggc tgtgatatta tcatcagaag atgattgtga tgatgattgt
     4321 acatgtggtg gttgtccaag ttgtgatgat agagagtaaa aattcataag aattaaaact
     4381 gcctatgatg cctatgagat attttgcttc ataggtagtt ttatttttat gaggaagggt
     4441 attatgtatt ttggttttgc aatacttgga caaaaatagt acactagaat ttatagaata
     4501 ggattttgga gggatgtaaa tatgaaaata agtaaaaaat tactagataa tatacaaaga
     4561 tttgaaaatg ttataaatta taaatttaaa aataaagaat atatactaga agctcttact
     4621 catagttcat attctaatga aaataaaaaa tataatttta acgaaaggtt ggaattttta
     4681 ggggattcgg ttttaggcat agtgataagt gattatttat ttaatgaaga agctaatctg
     4741 cccgaagggg aactaactaa acttagagca aatatagttt gtgaagattc gttaagtgaa
     4801 gtagctaatg atataaattt aggtattcat atgttattag gtagaggaga agaagcaaca
     4861 ggaggaagac ataggacttc aatattagca gacgcatttg aagctgtaat agctgctata
     4921 tatcttgatg gaggttttga aagtgcaaga cagtttatac ttcatcatat ggaaaatata
     4981 atttatgatt caagaaaagg aaatatattt agagactaca aaacgcatct tcaagaagtg
     5041 ttacaaggga atggagaaaa taatatctgg tacaggctta tagaagaaaa aggaccagac
     5101 cataataaaa gatttgtaat ggaagttgga attaatgacg atgtattggg tataggagaa
     5161 ggaaagagta aaaaagaagc tgaacagtta gctgctaaaa tagctttaaa gaaaaaatta
     5221 tgggaaaaat aaatataata tgtaaaaata taaaaagaaa tatttatatt agaaggatgt
     5281 tgtagatgaa aaagagaatt atacctatat ttgttcctca caaaggatgt cctcatgatt
     5341 gcatattttg taatcaaaaa aaaataacag gtgtgagtac tgatgttact agtgaagatg
     5401 caagaaatat aattgaggaa tgtttagaga ctatagataa agatgcagat gtagaaatag
     5461 cattttttgg tggtagcttt actgctatag atattgatat acaaaaaagt ttattatcag
     5521 ttgctaaaga atatgtagaa aaaggtcttg taaaagatat tagaatgtct acaagaccag
     5581 attgtattga tgaagatata ttaagtatgc taaaagaata taagacaagt ataattgaac
     5641 taggagtaca atccttagat gaaaaagtat tattagatag tataagaggt catcaatctg
     5701 aaattgtata caagagttca aagatgataa aaaatagtgg aatcaaactt ggattacaga
     5761 tgatgattgg tcttccagct gacacagaag aaaagtgtat atttacagca aaaaaatttg
     5821 tagagctaaa accagattgt gttagagtct atccaacttt ggtagtaaaa gatacaggtc
     5881 ttgaaaagtt aatggaacaa aataaatata atccatttac tcttgaagag agtgtaaata
     5941 tagtaaaaaa agttcttgta ctgttttatg ttaataatat caatgtaata agagttgggc
     6001 ttcaagctac tgatgatata caaattggaa aagctgtttt agcaggacca tatcatcctg
     6061 catttagaga gttggtagaa gcagatatga taaaagatta tttagaattt gttattttgc
     6121 aaaataaaaa tataaagcaa atgttggtca aatctaataa aaagaatatt tctaaaatca
     6181 taggaaataa aaaaacaaat gttaaatata tggaagaaaa atttggtgtg cttttaaaaa
     6241 cacaagaatc cgatttagat ataaatcaat tagaaattgt gttagatggg aaaagtttaa
     6301 ttagtgctaa tatgagagac attcatagaa aattgtatga catttatgac ctttagtttt
     6361 aataatgaag ggagagaaaa gatttgtatt taaaaagact agagttaaag ggatttaaat
     6421 cctttccagt aaaaacagat attattttta aagaaggaat aacagccata gtaggaccaa
     6481 atgggagtgg aaaaagtaat atatctgatg ctgtaagatg ggtattagga gaacagagta
     6541 ttaagagtct tagaggtgat aaactcgaag atgtaatatt tgcaggtaca gatacaaaaa
     6601 aacctatgaa ttattgtgaa gtggctctta ctattgataa ttctgaaaat caattggaat
     6661 tagattttac agaagtaact ataagaagaa gggcttatag aaatggtgaa agtgaatttt
     6721 ttctaaataa taaaagttgt agattaaaag atataaaaga agtcttttta gatactggta
     6781 ttggaaaaga tggttattca atcatagaac aaggaaaagt agatgaaata ttaagtaata
     6841 atcctttaag cagaagaaaa gtttttgatg aagcttgtgg catatcaaaa tatagatata
     6901 aaaaacagga agcagaaagg aatttaagta atacaaagga aaatttagaa agaatagatg
     6961 atgtatatat agaaattgaa aatcaattaa agcctctttt taatcaacag acaaaggcaa
     7021 aaaagtactt ggaaataagt gaaaaattaa agatacttga agtaaatagc tttataagag
     7081 aaattgaagg aatagaaaaa gaattaagtg aggttaatga acatcgtaag gttattgaaa
     7141 aggagctcaa tgaaaaagaa gaacaaaaaa atgttgtaga gaaaaagcaa gaagatatta
     7201 ataaagaggt agaggtatta caagatgtaa ttgaaaaatc tgtagattat ataaattcaa
     7261 ttaaaggagt tatatcaaaa aaagagtccc aaataaattt aattaaggaa agaataagaa
     7321 attttacgaa tgaaattagt agaaaaaatt tagaaataaa tgatataaaa gagaagctaa
     7381 atgaaaataa gcaatacata aaagaattag aaagtaataa attatcaggt agtgaagaac
     7441 tatctacctt gcaggaaaat ataaaagtat tggaggcaag taaagataaa caaaaaatta
     7501 agttagaatc cctaaataat gagatagaat tattgaaaga aagtataatt gatatattaa
     7561 ataagaaaca agagtttagt aataagctgt ccactttaaa cgcaaataaa gaaaatatga
     7621 atatcagaga tgaaaatata aattcagaga taacagagtt aaacaaaaat attgaaataa
     7681 aatcatctga attagatact ataaataaag aatttaatat gcaaaatgaa aatttaaaga
     7741 atgtaaataa tagacataaa gaattatcaa ttaatttgca ggattcaatt agtgaacaca
     7801 ataaactaga agatgaaata cagaaaagta aatataattt aaatggatat aattcaaagt
     7861 taaatgtcta tatagatatg gaaaatcatt atgaaggatt taatagagga gtaaaagaag
     7921 tactaaaaaa taaaaattta aaaggagttc atggagcatt aggacaaatt ataaatgtac
     7981 ctgaaaaata tgagaaatct atagaagcag ctcttggtgc ttatatgcaa aatataataa
     8041 ctgataatga gtttagtgct aagtctgcaa taaactatct aaaacagaat aatttaggta
     8101 gagtaacatt tcttccatta aatataatta aatcaaataa agtaagtcta ggaaatttaa
     8161 aagcaaatac taagttcata ggaatagcaa gtgatttaat tacatttgat gaaaagtata
     8221 gaaatataat tgaaaatata cttggaagaa caatacttat aaataatata gatgagggca
     8281 taaaatttgc aaaagaaact ggtcataggt ttaaaatagt aactttagat ggtgaaatct
     8341 taaatccagg aggttctttg actgggggaa gtctaaaaac taatgggaat atactatcga
     8401 gaaaaagata cattaatgaa tatacagaaa agataagtaa tatcaagaat gaaatttcac
     8461 atctagaatt aaaaagagaa tctttagaca aagatgtgaa aaatatcaaa aatgaaattg
     8521 attcacatga aagtaaaata aaagatttag aaaaaagtat aataataaaa agtacaagta
     8581 taaagaatgt tgaatctgaa attgaatcgt taaaaggtag tattactaaa ttagaaaatg
     8641 agaagaatga tttaaattct aatctaaatt atacacttga aaaaagcgat gatgttagaa
     8701 aagatatgga agagttagat gatttataca ataaaaataa agaaaaaatt gatgcattga
     8761 atgaggaaat aaaaagatac aatgatttat atgataaaga aaaatctgaa tttgatgaat
     8821 tgaacttaag tttagtgaag aaaacagaag cttataatag tatagttaga gatataaaaa
     8881 gaatatctgg tgaaaattgt gagctagaag aaaagaataa acaattagaa gagtcattaa
     8941 attatgaaga acatgaaata attaaattac aagattctat agccactgaa gaaaaagaaa
     9001 aagaaaattt aacaaaacaa ctaggggata gtaatagaaa tcttgaaact agaaaaatag
     9061 caaaagatga tttaaaaaat agttttgatg aaattaataa agaattaaaa actatagata
     9121 gacagcatat agagcttaaa gaaagtttat ttaaagttgg tggaagatta gaaagattaa
     9181 aaactagtca agacacatat ataaataagt tgtttgagca atatgatatg actttagtac
     9241 aagcattgga aattaaagat gaagatttgg atatagatag aaaattctta gaaagtctaa
     9301 agagagaaat aagaagtctt ggaaatataa atataggctc tataaaggaa tatgaggaaa
     9361 taaaagaaag atacgatttt tatagcgaac aaaagcaaga tttagaagag tctatggaag
     9421 agatagaaaa acttatacac actttagaag aaaatatgaa atctgaattt gaaattaagt
     9481 ttgaagaaat aagtaaaaat tttaaatatg tatataagag attatttggt ggaggttgtg
     9541 gagaattaac tattttagat aaagaaaatt tattagaaag tgatatacta ataacagctc
     9601 aacctccagg gaaaaaaatg aaaaatttaa atctattatc tggtggagag aaagctttaa
     9661 cagcaataag tatattattt gctatattga ttacaaagcc aacaccattt tgtatattag
     9721 atgagattga agcgccactt gatgatgcaa atatatttag atttggagaa tttttgaagg
     9781 atttatctaa agagactcaa tttatatcag taactcatag gagaggaact atggaagctg
     9841 ctgattatat ctatggagtt acaatgcaag aaaaagccat ttcaaaagtt attagcttaa
     9901 aattaaaaga agctcaggaa ataacagata ttatataact tagattgaca taggaggaat
     9961 aacagtgtta aaaaaattat ttggttttgg aaaagacaag gaaaaagaaa tagaaaaaaa
    10021 agatgctgaa gaagaaatag aagttgaaga cagtgttgat agtttagaaa acttggaaga
    10081 aaccattttt tcaggacttg aagaagaagt gatagataaa gtggaagata ttgaagaaaa
    10141 aactgaggaa agtgtcaata atgaaggcac tgaagaaatc gaaaattttg aaaaaataga
    10201 agttgagatg gatagtaatg caaaagaagt agaaaatatt tcaaataatg attctcaaga
    10261 agagataatc gaagaattag aaaattatga tgaaattgag tctaaagaga tagaagataa
    10321 agaagataag aaagttaact tgtttgaaag actaaaacaa ggattgacaa aagcaaagca
    10381 aggaataact gatagaatag atgaagtttt aaaatcatat acaaaaatag atgaagaatt
    10441 acttgaagat ttagaggaaa ttttaataac agcagatgtt ggtgtaaata caactatgga
    10501 tataattgaa agactaagag ataaaataaa gcaaaaaggt attactgaac caataaaggt
    10561 aagagaagaa cttaaatcta tagttgaaga tattcttaca aatgaaaact ctacactaga
    10621 tatagaacca gcaccatgta taatattaat ggtgggtgta aatggggtcg gtaagactac
    10681 tacaataggt aaactagcaa atagatacaa aaaagatggt aaaaaagttt tattagcagc
    10741 agcagataca tttagagcag cagcaacaga gcaattagaa atttgggcaa atagaaccaa
    10801 tgtagacata ataaaacatc aagagggagc agaccctgga gctgtagtat ttgatgctat
    10861 aaaagctgca aaagctagaa aaacagatgt attaatatgt gatacagcag gaagattaca
    10921 caataaggct aatcttatga atgaacttgg aaaagtattt aaaatagtag atagagaatt
    10981 tccagaagca aaacgtgaag tacttcttgt ggtggatgct acaacaggtc aaaatgcagt
    11041 tgttcaagca aagacattta aagaagttgc agatataaca ggtatagtac ttacaaaact
    11101 tgatggaact gcaaaaggtg gagtagtcct tgcagtaaaa tcagaagtag atgtacctgt
    11161 aaaacttatt ggagttggag aaagtgtaga agacctacaa gatttcaatg caaaatcatt
    11221 ttcagatgca ttatttggta actaatgttc ttttaaaaat aatggttgac aagagtgttg
    11281 ttatgatata aaatacaata gtgtaaagta aataaccttt acagtatact gctaggaagt
    11341 gtaagatatg aatatagaaa aaatggtcga aattggattg ttatttgaac agtataaaga
    11401 attattaact gacaagcaga aagaaatagt tgctctatac tatgaagaag attattcttt
    11461 aggtgaaatt agtgaaaatc tgaatgtatc aagacaaggt gtttatgata ccttaaagcg
    11521 ttcagaaaaa atactaagag attatgaaga aaagttacac ttagtatcaa agattcaaga
    11581 gcaggaaaaa aatattaaga ttataaaaga taaaattatt gacattaaag aagatttatt
    11641 gcacaataga gattgtgcta atttaatccc taagctagaa aatatagaag atgtatgtag
    11701 ggagatgata aaatgatatt tgaaggattg tctgataaac tacaaggggc atttagtaag
    11761 ttaaaatcta aaggaaaact tacagaagta gatgtaaaaa atgctatgag agaagttaaa
    11821 cttgctcttc ttgaggcaga tgttaacttt aaagtagtta aagatttcat aaaaaaagtt
    11881 caagagagat gtgttggaca agaagttatg caaagcttaa ctccagctca gcatgtaatc
    11941 aaaattgtaa atgaagagct tacaagttta atgggagatg tacaaagtaa agtaatgatt
    12001 tcctcaaagc ctcctacaat tttaatgatg gttggattgc aaggtgcagg taagactact
    12061 acagcaggaa aacttggagg atattttaaa aagcaaggta aaaaacctct attagtagct
    12121 tgtgatattt atagaccggc agctataaag caacttcaag ttgttggaga aaaattggat
    12181 atacctgtat tcaatatggg agataaagaa aatccagtaa atattgcgaa agcaggttta
    12241 agccatgcga ttaaaaatgc taatgattta gttattatag atacagctgg tagacttcat
    12301 atagatgaag tacttatggc tgagttaaag tctattaaat cagaagttaa gccacatgag
    12361 atacttttgg ttgtagattc tatgacaggt caagatgctg taaatgttgc agaaagcttc
    12421 aatgaggcac ttggtgttga tggagtagta ttaactaaat tggatggaga tactagaggt
    12481 ggtgctgcac tttcaataag agcagttaca caaaagccta taaaatttat ggggatggga
    12541 gaaaagctag atgatataga acctttccac ccagatagaa tggcatcaag aatattaggt
    12601 atgggagatg tcttaagttt aatagaaaaa gcacaagaaa atatagactt agagaaagct
    12661 aaagaattag agcaaaaaat taaaaaacaa gagcttgatt ttgaagattt cttacaacaa
    12721 atggaacaaa tacaaaatat gggacctctt gacaagatac ttggtatgat acctggtatg
    12781 ggaaatgtaa aagaccaact tggagatgta gatttgaatg gcaaagaaat gaagagaaca
    12841 aaagccataa ttcagtcaat gactacatat gagagaagaa acccaggagt attaaatgct
    12901 tcaagaaaga aaagaatagc taagggcagt ggaactagtg tacaagatgt aaatagactt
    12961 ataaaacaac ttaatgaaat gaaaaaaatg atgaagatgt ttgctggttc acaaaagagt
    13021 atgaagaaaa ggggcggctt aggcggttta ccttttttta aataaaatat aaaaaagtaa
    13081 aaattattcg gaggtgactg taatggcagt taaaataaga ttaaaaagaa tgggagctaa
    13141 caaaaaacct ttctacagaa tagtagtagc tgattcaaga gctccaagag atggaaaatt
    13201 tatagaagaa ataggatact ataatccaat ttctgaacct aaacaagtaa gaataaatga
    13261 tgaaaaagct ataaaatggt tagctactgg tgctcagcca acagaagtag ttaaaaaatt
    13321 acttgtaaaa aacggagtaa tagaaaaatt cgaagcttct aaacaagcaa aataattttt
    13381 tcttggcaat tagtttaagt aagggctcat tgctaaggtt actttaggag atgaaatata
    13441 tgaaagagct agttgtagat atagctaagg ctctagttga taatcctgac tcagttgttg
    13501 ttgaggaatt tgaagataat gatggtatcg tcttaaagct tacagttgct caagaggata
    13561 tgggtaaggt tattgggaag cagggaagaa tagctaaagc tataagaact gtcgtaagat
    13621 ctgttgcaaa tagagaaaat ataaaagttt ctcttgagat agtataaaat aggaggatta
    13681 ggtataatgc ctagtccttt ttatataaat gcgtagttta agattttatg atgatatgac
    13741 cacataagat taaatttttt aagaaggtga taacatgaaa gaaaaattaa ctcattttaa
    13801 ggtaggtcaa atagtaaata cacaaggttt aaaaggtgaa gtaagagttt atccacttac
    13861 tgatgatata gagaggtttg atgaattaaa agatttctat ctgggaaaag atttaaataa
    13921 taaattggct gttgaaaaag taagatacaa aggttctatg gttataatga agataaaagg
    13981 catagacagc atagagaaag cagaaaaact aaaaaataaa tttatgtatg tatctagaga
    14041 agatagcaga gagcttgaag aaggcgaatt ttttatagca gatatgattg gaatggaagt
    14101 tttaactgta gatggaaaat atgtaggaat actagaagat gttcttcaat actcagcaaa
    14161 tgatgtttat gtagtaaaag gtgaagaaga taaagaattt atgataccag ctataaaaaa
    14221 atttgtacct acaatagata ttgatgaaag aaaaatgatt atagacccta ttaagggaat
    14281 gatagattag gtggtaatat gagatttcat ataatgacac tttttccaga aatatttaat
    14341 tcgtacatgg atgaaagtat aatgaagaga gcagttgaaa aaggtataat tgaggtttat
    14401 atatataata taagggattt ttctaataat aaacataaaa aagtagatga ttatccattt
    14461 ggaggaggag tggggatggt aatgactcct caacctatat atgacacata taagcatata
    14521 ataacaactc acaatataag taatccaagt gtaatttatc ttactccaaa aggtaaggta
    14581 tacaatcaat ctatagcaaa gcagatgtct ttaaaagaag atataatact attatgtggc
    14641 cattatgaag gagtagatga gagaatcatt gatttaatag ttactgatga aatttcaatt
    14701 ggagattatg tattaactgg gggagagctc ccagcactta taatgataga ttctatatca
    14761 agattaatac caggtgtatt aaaccaagaa gaatcatttg aagaagaatc atttaaagat
    14821 aacttattgg aatatcctca ttacactaga cctagagatt ttgaaggatt aaaagtacca
    14881 gaagtgcttt tatcagggaa tcataaaaag atagatgagt ggagaagaga agaatctata
    14941 aggataacca aagaaagacg atttgattta tataaaaagt ctaatgaaaa ataaaaatat
    15001 aatgaaaaat taatatatat gtatacaata aagtacctat aaatggaatt aaaaacataa
    15061 aagtgcttgt aataactatt gaagtatagt ataatataaa atgttgaaaa tacgggcgtt
    15121 cctctgttta cactggtcgg gtagttaaga acgtctatga tatctaggag gaagaaagat
    15181 gaatgaaatg ctaagagcaa ttgagcaaga acaattaaaa aatgaagttc ctaattttgg
    15241 acctggggac acagttaaag tacacgttag aattatagaa ggaaaaagag aaagaataca
    15301 agtattcgaa ggtgtagtat taaagagaca aggtggagga gcaagagaaa ctttcacagt
    15361 tagaaaaatg tctttcaatg ttggtgtaga aagaactttc ccagttcact ctccaaaaat
    15421 agaaaaaata gaagttacta gaaaaggtaa agtaagaaga gctaaactaa actacttaag
    15481 aggtagagta ggtaaagctg ctaagataaa agaagctaga aataaataat taaaaacttt
    15541 acttataaat ggggggctga tttatcagtc ctctttctat tttaatgaaa attacagcaa
    15601 ataattatac tagaaataaa tagaaatatt tgttgaaaat atacattatt tatctatgaa
    15661 tttatagctt ttaaaatgaa aaataaaatt ggtaaatatt ggattatatt ttgtaacgat
    15721 attaaaaata agagaacagt attatgtaaa gtatgacgtt aaaaagatga caatattata
    15781 gtttgtgaag atagattttt taagtggaat tagaatataa taatataata ttaataagga
    15841 gtgatacatg tgtcaaatga atacgaagag tatcttatgg atgacaatct acatataaac
    15901 tggtatccag gtcatatgaa aaaaactaag gagttagtaa aaaataactt aaaattgatt
    15961 gatgtagtaa ttgaactttt ggatgctaga atcccgttta gtagtaaaaa tccagacata
    16021 gacaggcttg taggagataa gccaagagtt gtcgtactta ataagagtga tatggcagat
    16081 agggataaat tgaatcaatg gattgaatac tataagaaaa ttaatatcaa agctatacca
    16141 gtagatacaa taaaaggtgt tggtataaat aatataatcg aagaatgtaa aaatgtaact
    16201 agagaaaaaa tgagttcttt aaaagacaaa gggagaaaag aaagagctat acgaataatg
    16261 atagttggag ttccaaacgt tggtaaatcg tctcttataa acaaattaac aggtagaaaa
    16321 agcactcaaa ctggagataa gccaggagta acgaaaggga aacaatgggt tagattaaag
    16381 ggaaatcttg aacttttaga tacacctgga atactttggc ctaagtttga agaccaagaa
    16441 gttgcattaa atttagcatt tagtagagct ataaaggatg agattttaga tactgagact
    16501 ttggctttaa gacttataga aaaacttatg aaaatagaac ctgagaagtt gaaagccaga
    16561 tataagttag attgtctagg agaaactcca attgaaacta tggatatgat tggccataaa
    16621 agagggttta taacaggtaa aaaagaacta gattatacac gcatagccgc aactgtatta
    16681 aatgaattta gagatggaaa aataggaaat ataacattgg aagttccaga aaacgttaaa
    16741 agatagattt taaaacaaaa tcaaagattt aatgttaaaa aaataaaaaa gccttttagc
    16801 aaaggctttt ttattttttt gaaatataga caaatatctc catataaaag tataaattag
    16861 gttagaaaac ctttaaaaat attaatttaa gacaaataca cctaatattt tgaaaattaa
    16921 aatgagtgtg attattttaa attttagca
//
LOCUS       DAELSY010000083        16913 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.083, whole genome shotgun sequence.
ACCESSION   DAELSY010000083 DAELSY010000000
VERSION     DAELSY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16913
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.083"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(35..2155)
                     /gene="feoB"
                     /locus_tag="KPG09_002406"
     CDS             complement(35..2155)
                     /gene="feoB"
                     /locus_tag="KPG09_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG0967009.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(2181..2408)
                     /locus_tag="KPG09_002407"
     CDS             complement(2181..2408)
                     /locus_tag="KPG09_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0967010.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            complement(2735..3175)
                     /locus_tag="KPG09_002408"
     CDS             complement(2735..3175)
                     /locus_tag="KPG09_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0967011.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     INLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            3327..4316
                     /locus_tag="KPG09_002409"
     CDS             3327..4316
                     /locus_tag="KPG09_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBG0967012.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     misc_binding    4468..4720
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4792..6000
                     /locus_tag="KPG09_002410"
     CDS             4792..6000
                     /locus_tag="KPG09_002410"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBG0967013.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     gene            complement(6061..6942)
                     /locus_tag="KPG09_002411"
     CDS             complement(6061..6942)
                     /locus_tag="KPG09_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG0967014.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPGRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            7328..8272
                     /locus_tag="KPG09_002412"
     CDS             7328..8272
                     /locus_tag="KPG09_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG0967015.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLI
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            8495..9040
                     /locus_tag="KPG09_002413"
     CDS             8495..9040
                     /locus_tag="KPG09_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG0967016.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            9131..10279
                     /locus_tag="KPG09_002414"
     CDS             9131..10279
                     /locus_tag="KPG09_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG0967017.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQNSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSKKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            10332..11597
                     /locus_tag="KPG09_002415"
     CDS             10332..11597
                     /locus_tag="KPG09_002415"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBG0967018.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(11883..13115)
                     /locus_tag="KPG09_002416"
     CDS             complement(11883..13115)
                     /locus_tag="KPG09_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0967019.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(13118..13792)
                     /locus_tag="KPG09_002417"
     CDS             complement(13118..13792)
                     /locus_tag="KPG09_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967020.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLVN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            complement(13800..15029)
                     /locus_tag="KPG09_002418"
     CDS             complement(13800..15029)
                     /locus_tag="KPG09_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG0967021.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            complement(15141..16145)
                     /locus_tag="KPG09_002419"
     CDS             complement(15141..16145)
                     /locus_tag="KPG09_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0967022.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLINEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            complement(16169..16843)
                     /locus_tag="KPG09_002420"
     CDS             complement(16169..16843)
                     /locus_tag="KPG09_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0967023.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
BASE COUNT         6077 a         2292 c         2532 g         6012 t
ORIGIN      
        1 tctctaatat atgtctaatg tcagttaata ctaattatat aaataaattg taaactgtag
       61 ttgttaaaaa acaaacaaca aaagcaagta tagtaggcat taagaatcct aaggcagtcc
      121 attttaaact accagtttct ttttttattg ttaaaagtgt tgttgcacaa ggccaatgca
      181 gtaaactgaa cagcataaca tttaaagctg tcaaataagt ccatccatgt tctctaagaa
      241 cttgacctaa agcactaaag ctatctaatt ctatcataga accagttgct aggtaagcca
      301 ttaaaagtat aggtacaact atctcatttg ctgggaatcc aagaataaaa gcaagtaata
      361 taaaaccatc aagaccaatt aatttagcta aagggtctaa aaagtttgca acatgtgata
      421 atatgcttaa atctcctata tatatatttg caaatatcca agttatgaca ccagcaggta
      481 tggctaccat tacagccctt cctaaaacaa atattgttct atctattata gaggtatata
      541 aagttcttcc tatttgagga actctataag gaggaagttc taatgtaaat gtagacggaa
      601 ctccttttaa tagcgtctta gaaagagtat aagaaaccaa caatgttatt ataacaccta
      661 gaattatcag taaagttatg caaagtgcag ttgctacgct tgatacgaaa gagttggtta
      721 taactgagct aaaaaatatt gtagatatag ctatcaaagt aggaaaacga ccattacaag
      781 gaacaaagtt gtttgtaagt atagctataa gtctttccct tggagagtca attatcctac
      841 aaccaataac tccagctgca ttacatccaa atcccataca catagtcaaa cactgttttc
      901 catgtgcaca agctttttta aataggtggt ctaagttaaa tgcaactctt ggcaaatatc
      961 caaaatcctc aagtagggta aataatggaa aaaatattgc cataggaggt agcattacag
     1021 agattaccca tgctaatgtt ctgtataatc ctaaaaccaa catatcattt aaccatgatg
     1081 gacaattaat actatttaat atacccgaaa tacttggctc aaatccgagt aataaattag
     1141 ataataaagt ggatggatag ttagcccctt ctatagtaat ccacaatata gtacctaata
     1201 ataagagcat tattggaagc ccaaatattt tagatgttaa tattttatct actttttctt
     1261 ctctatcagt tgatttttta gcaacattag aacaacactc atcactcagt tttttagcat
     1321 aatcatagtt gattttagta aactcatctc ttattttctg tttatttata ttgtctggaa
     1381 tctttttctt tacttcatta atagcatcta tagaatcttt gtctatataa ttactcatag
     1441 attcaaatat actttcatcg ccatcaataa gtctaagtcc caaccatcta gaatttatac
     1501 ctggtataat attatccaat tctggttgaa ttgattttac cacattctct atattttcat
     1561 tgtatctaac aggtttatta tttaatttgt atttgtcaaa agatacatca tttaaagtat
     1621 ccaatagttc atccatacca gagccatttc ttgcagcagt taaaataact ggaataccaa
     1681 gagaatcctc taaaagtttt ttatcaatag taatgccttt ttttctagct tcatctatta
     1741 aattaataca caaaataact ttatctgtaa gttccatcac ttgaaatact aaatttaaat
     1801 ttctttcaag acatgtagca tcacatacta ctattactgc atctggatta ccaaaacata
     1861 taaaatcacg agctacaatt tcttcttgtg ataaggcaaa taaagaatat gttcctggta
     1921 aatctattaa tgcatattcg gtatttttat atttaaaatt accacgagct gtagctacag
     1981 tttttcctgg ccagtttcca gtatgctgtc taagccctgt aagatgatta aatacagtac
     2041 ttttacctgt atttggattg ccagccaatg caattacaaa ctggtcttcc ttattgtcta
     2101 tatcaaacat atcttttaaa gaactcattt tagttgagtt atgtgtcaac cccatattaa
     2161 tatcctcctg ctagaaattt ttaaatgtca gaaactaata ttaagctact ttcttcttgt
     2221 cgtagagcta tcttactacc tctgatttta tagacagtta aattattttt tggacctttt
     2281 ctaactacat ctataacagc tcctctagta agaccaagcg ctaacattct ttctcttaaa
     2341 ttaccactag ataatatatc ttcaactttg acagtttttt tcacttgaat atcgttaagg
     2401 tttttcatag tagcacctcc atgcttgaaa tttagcctgg gctaatcttc ttattctaaa
     2461 ctatgcatta tattattacc ttgttacttt tacaaaaaaa agataggcaa gatgcctatc
     2521 atacaaagtt gggtataatt cttacttttg gggaaagtag aatatagaat aaaaattaac
     2581 actttgaatc catataatta ttgaattttg ataataaaaa agtcagaaag caaaagcctc
     2641 ctgactctat gtaaggtatt ttcaaatacc taaatatcaa ttcaatattt aatttatact
     2701 taaattatat attaacaata ttagcttgtc aacactataa tctcatttta atatgttcaa
     2761 tacctgcttc gtcgtatact tctgagattt ctttaaaacc acaagataag taaaggttct
     2821 ttatataagt ttgtgctgat aaggttatat tattttcgtg taagttaatt tttataaaat
     2881 ctatagcaca atccatcatt tgttttgcta atccttttct tctatgacta gaaagaacta
     2941 aaactctacc aatggaaaca tcattataag agctgtattc tttaggaatt attctagcat
     3001 aagcacaaat caatccattt tcttttaaaa atatgtgata agagctttta tctatatcat
     3061 cataatcgtt taatgaaaat atcttctgtt cacaagcaaa tacctcatat cttgattttg
     3121 ctatttcata aaattcatct aaactcaaat cattaaagtg tttaatttta aacatagtaa
     3181 ttacctccta gcatcttaaa aaactgaata ttaagataca ttataccatt tttaacataa
     3241 atacctttat taaaatttat atttattaaa aatattttta aaaagtgata tactatatta
     3301 aaaacaaaac tttagttaga aaatttatga tttatggagg cagtgttttg aatactacta
     3361 aaaaattaac tgaagcagct ttattgtcat ctttattcat agtagtaaca attatagctg
     3421 tgagtactgg atttggatat gctatttact tagattttat agtgccagta tttttttgta
     3481 taatctgctt gaaatgtgat ttaaagtata ccattttatc tggaattact tcacttttaa
     3541 taatttctct ggtacttgga aatctaggaa ctgctatttg ggcaagtcag agtgtattac
     3601 ttggtataat gtgtggctat ctaataaata agcctacaat ggttatggat gatttagtat
     3661 atggttcagt atttggggtt attgtaatgg ttttcattga tatatatgct tcaacattaa
     3721 taggttatag ttttatgaaa gaatttcaag gatatagcaa atggatttat tttaatgggt
     3781 atacaaattt tatttattat ttaatgatag cattgtttcc ttttggtatg gtgttttgca
     3841 tatatttatt aagtttaatt ttagggaata aattgcatat tttagattct aattctttaa
     3901 aaaaattttt aatatttaag aattttagaa gtttaaacca atttttatgt tgctcaaaaa
     3961 aagcttttta catatgtgtt tcttatctag ccatttttga agttctaaaa ctgttgaatg
     4021 ttaaagttga ttctgtttat ttaaaaacaa tctttatatc aataacctat ataagtgtct
     4081 attttatcat tagagactca tgtatgtcac tacaaaactt tataatagct aagtttagaa
     4141 aattattata tgctagaatt ttatttttga taataatttt attgctgttt tttatatttg
     4201 atgtaacagt tattagttta ataattataa acgcattttt aaataagaaa ataaatataa
     4261 gattgagtca gagtaatata gtcaataaac aaataaatct tttgattgaa aaataaactt
     4321 tgggtattgg taaaaaatat aaaaaagtag tataatgtaa aaaaacatgt atataataat
     4381 aaaaaaggtt gacaaaatat aaaaataata atatattatg agtaagttaa gaaaaatttc
     4441 ttgtaattga tgaaataatc tgaatatata aatgctatga agagaagaag tagggctaga
     4501 ttttatttaa cagagagttc cagtttggtg aaatggaata aattgattta gtttgaatac
     4561 atctcagagc actatctctg aaagataaca accattaaga gatatcggat tagtacacgt
     4621 tatagtacac gagtatatgt aattgtactc taagagatta atatcgtgag atattgatga
     4681 actaaggtgg taacacgtaa gcaatgcttt cgtcctttta agaggatgaa agcttttttt
     4741 attataaaaa aaaaattata gattatataa atattttagg aggaatgaaa aatgaaatcg
     4801 atagacgagc aaatgcgaat aattatgaaa ggtgtagatg atttaataga tgagaaagag
     4861 ttaagggaaa agcttattaa atcagaaaaa gaaggaaagc ctatgatagt aaaattgggt
     4921 ctagacccaa gtgctccaga tatacattta gggcatacag tagttcttag aaagatgaaa
     4981 caattacaag atttaggtca tcaaatagta ataataattg gagattttac aggaaaaatt
     5041 ggagacccaa ctggaaaatc taaagctaga aaagctttaa caacagagca agttcttgca
     5101 aatgcaaaaa cttatgaaga acaaattttc aaagttttag ataaagaaaa aactattgta
     5161 agatttaata gcgagtggtt agccaagtta aattttgaag atgtaataaa attagcagca
     5221 actattactg ttgcgagaat gttagaaaga gaagatttta aaaagagata tgaaggacaa
     5281 atgccaatat cagtacatga gttcttctac ccattaatgc aagcatatga ctctatagca
     5341 ttagaagctg atatagaact tggtggaact gaccaaagat tcaatttact aatgggaaga
     5401 tcattacaaa gagaatttgg tatggagtct caaatagtaa taatgatgcc tttaatagaa
     5461 ggattagatg gtaaagaaaa aatgagtaag agtttaggta actatattgg aatagatgaa
     5521 gaagcgggaa taatgtacca aaaatctatg gaaattcctg atgaacttat aattaaatac
     5581 tacaatttag tgacagatgt tcatcctgat gaagttaata aaatagaatc tcagttgaaa
     5641 gagggttcag taaatccaag agatataaag atgaacttag caagagaaat agtaacatta
     5701 taccatggag aagaatctgc taaagaagct gaagaaagat ttaaatctgt attccaaaaa
     5761 ggtcaaatac ctgaagatat acaaacaatt caagttaagg aagatggatt tgacttaatc
     5821 gaagtactag tttctaatga gattgttaag agtaagagtg aagttagaag attagcttct
     5881 caaggtggag taaaggtaaa tggtgaaaaa gtagaggatt taagcactat tgtaaaagag
     5941 agtgaattag ttgttcaaat aggaaagaaa aaatttgtaa aaatcgagct agtaaaatag
     6001 agtaaagtaa aagcaagtct attgagtata aaatgtacat tcaataaact tgctttttta
     6061 ttatattaaa attttaaatt gataaccatt gtctttaaaa tatgttataa tttggtccaa
     6121 tgcatttact gtttcttgtt ttccataagt atcatgcatt aaaagaacaa ccatttcttt
     6181 gccttcagaa gatttctttg catgttcaaa taattcttgt gcatttttct ttttaccttc
     6241 tgcatcagca tttaaagcgt tccaatcaat tgatgccatg tttttctctt ttaagtagtt
     6301 tcctagaggt tccatatttt tccatgacat atacccgcct gggcatctaa ctacatttga
     6361 ggaaaaattc tttcctaaga cttttttcat agcttcatca gttttgttaa gttcatttac
     6421 gaatgtatcg agatttaggc ttctgccagg atatagtttt ttatagtcat gggtataaga
     6481 gtgatgggct attgcatttc cttcgtcaaa tgttcttttt aaaatttcat tagctccttt
     6541 tctttcaaga gaatcaccct taataaagaa agttcctcta acaccatgtc ttttcaagat
     6601 atctagaact tgtggagtat tagtagtaga tggtccatca tcaaatgtaa gaaagactat
     6661 cttttctcca ttgtttgaat agtcgtattt gttaagtttg tcccaaacta tttttgcgtc
     6721 atatccatat tcttttacag cttctttttt tgcaccaata ataggttcat ttttttcttt
     6781 ttcaattctt tctttttcta atctttcagc tttaagtctt tcttgagttt ctttcatttc
     6841 tttttttcta gacatgtccg cccatgccat agaaaaaata ccaataacta ttacaacaag
     6901 tatagtaagt gtaacagttt tcttttttct tttttttacc ataattacag cattccccct
     6961 tttgtcgaat tgtatcaaaa atatattata attatatcga agaaagtaga aaaagtaaac
     7021 cgacaaagtt gtaactaaga taactaaagt gtaacttata tgacattatt tgcataatat
     7081 taaagtatca tatatattaa aaatatctat aaaaaaattt agtataggta aaaaaatata
     7141 aaaaatataa aaatttatta aaaaggtgtt tacaaaaaat gattaatata ttactgttaa
     7201 atagtggata taaatttgag ctcaaaatgt atgtaaattt agatttaaaa tactgaaaaa
     7261 atttactata ttaatttata gtaatgctta tagaaaaact ctcttgctgt ttagggggtt
     7321 tataattttg caaaaaaata aaaaggaggg ggctttattg aaaaaatact ttggagaaat
     7381 aggactaata tttatagcaa ttatatgggg aagtggcttt gtagcaaccc aatttgcatt
     7441 agacggtggg cttacacctc tacaaatcat aactttaaga tttttcttgg cagctataat
     7501 tatgaattta ctgtttttta aacagattcg tgccaatatg ggtaaaaaat tattaaaagc
     7561 aggagggatt ttaggaatat ttttatttct agcattcaca gtacaaacta ttggacttat
     7621 gtacactaca ccatctaaaa atgcatttat aacagcagct aatgttgtaa tagttccgtt
     7681 cataggattt atactttata gaagaaaact tgataaaata ggaataataa gtagtttggt
     7741 agctcttata ggaattggga tactttcttt agaagctgat ttctctatta attttggaga
     7801 ttttctaaca cttatatgtt cattcggatt tgcatttcat atattcttta caagtgagtt
     7861 tgcaaaagat aacaacccaa tggcattgac agctattcaa tttacagtag cgtttttaat
     7921 gtctgttgta gttcaaacat ttgcaggtca attaaagatg gaagctgaat tatctggtta
     7981 tatgggaact atgtatctag ctgtatttag tactacaatt ggatttttat ttcagactat
     8041 atgtcaaaaa agagttgatg gaactagaac agctataata ttatccacag aggctgtatt
     8101 tggtacaata ttttctataa taattctaaa ggaacttatt acagcaaaat tgattattgg
     8161 tagtatcttg atatttgttg caatcataac tgcagaaact aaattatctt ttttaaagag
     8221 caaaaaagtg aaacttaaag attcagagga gtcttcatta gaaagtattt aatatgacaa
     8281 ctatttccta attaatcaaa taagttatgt attatgtaga aatttgacat caaaacttgg
     8341 ttgatactgg tgtttcaaag tgataaaatt aattaggaaa taaattttat ttttagtgtt
     8401 taagctataa agtctggggt atatatattc ttgtagcaag ttattaaact taaaaataaa
     8461 aatttaatta tataaaattg ggaggaaatt aattatgaaa aaatttgttt gtacagtatg
     8521 tggatatata catgaaggag atgctgcacc agcacaatgt ccagtatgta aagttggagc
     8581 tgataaattt gaagaaatga aaggcgaaat ggtttgggct gatgaacata gaataggagt
     8641 agctcaaggt gtagatgcag aaataatcga aggattaaga gctaacttta ctggtgagtg
     8701 tacagaagta ggaatgtatt tagcaatgag tagacaagct gatagagaag gttatccaga
     8761 agtagctgaa gcatataaga gaatagcttt tgaagaggct gaacatgctg ctaaatttgc
     8821 agaacttctt ggagaagttg tagttgcaga tacaaaagaa aacttaagag ttagagttga
     8881 tgctgagtat ggtgcaactg atggaaaatt aaaattagct aagagagcta aagaattagg
     8941 attagatgct atacatgata cagtacatga aatgtgtaaa gacgaagcta gacatggtaa
     9001 agcattctta ggattattaa acagacattt tggaaaataa taattaaaac atagattaaa
     9061 aggagaaact ttggtttctc cttttttaaa ttatacaaat aaactgttgt gatagaagga
     9121 tggagataat atgaagtcgg tatctgttag gagttgtaaa acttacaatt atgaagaagt
     9181 aaagttatct attgaaaaaa atttagagga tattggtggt atagataagt ttgttaaaaa
     9241 aggaagcaaa gtacttataa aaccaaattt attaatgaaa aaaaagcctg aagaagctac
     9301 aactacacat cctatggttg taaaagttgt atgtgaaaaa cttttagagt taaattgtga
     9361 tattacaata gcagatagcc ctggtggtcc atataatcaa aattctctta agagcattta
     9421 taaggcatct ggaattgaag aagtagcaaa tgaactgggc attaagttaa attatgatgt
     9481 ttcagatgtt aagatacata gtaaaaaagc acatgttcta aaatatatgg atattattaa
     9541 accaatagta gattcagatt atataataaa tctttgtaaa ttaaagactc atataatggc
     9601 aacctttact ggtggtacaa aaaacttata tggatgcata gcaggattga aaaaagcaga
     9661 gatacattat agatttccta cagaagagtt gttttgcgaa aatgtacttt tagatatttg
     9721 tgattatata aaaccaacat tgacaattat ggatggtata gtaggtatgg aaggtgatgg
     9781 accttcagct ggtactactc gagaaatagg agttttatta gcatcacaaa atccatatgc
     9841 tatagatgtt gttgcatgta agattataag tttacttcca aatagagttg ctacaattag
     9901 aggtgctatt aagagaggct atatacaaga agatttttca gatattgaaa ttttaggtga
     9961 agatataaaa gatttaatta taaaaaatta caagattcca aatgtaagta aggatttaag
    10021 attgttttcc gtaaagctac caaaattttt aaatgaacct atttcaaagc ttataacacc
    10081 aaagcctgtg gtaagatttc aagattgcat aaaatgtgga aaatgcaagg aagcttgtcc
    10141 tgcatccact atagagatag ggactaaagg tgctattata gacttaagta aatgtataag
    10201 atgttactgt tgccatgaac tttgtcctaa aaaggctatt gatgtcaaac gtaattttgt
    10261 atttaagtat gtaaaataat gtataatatt atatgagtct atataaatta aaaatataaa
    10321 ggagaaataa tatgatttta attaaaaatg gtagagtaat agaccctaaa agtcaaagag
    10381 atgagaatat tgacttaatt ataaaagaaa ataaaattta taaaataggc aagtttgatg
    10441 aaagtgatga atatgaaaaa ataatagatg ctagtggtaa tgtagttgct ccaggtttag
    10501 tggatgtaca tgtacacttt agagaccctg ggtttacata taaagaagat atagaaagtg
    10561 gagctaaatc tgctgctaga ggtggattta caactgttat atgtatggca aatactaatc
    10621 ctattgtaga taatgaagat acttttaatt atgttaaaga aaagtcaaaa aatgcatgta
    10681 taaatgtttt acaagcagca gctataacta aaggttttga aggtaaagag ttagttgata
    10741 tggaagctct taaaaaagct ggtgtaccag ggtttactga tgatggatta cctttaatgg
    10801 atagcaatct aattatggag gcaatgatta aggcaaaaga gttggatgta ccacttagtt
    10861 ttcatgaaga agacccttct ttagttggaa atccaggtgt aaatgcagga aaggtagctt
    10921 cagaactagg attaaaaggt gcatcaagtg tagcagaaga tgttatggta gccagagatt
    10981 gtatgttggc actaaagact ggtgctaaag ttgatataca acatataagt tcaggtgtat
    11041 ctgttgatat ggttaggttt gcaaaatact taggtgcaaa tgtagttgca gaagcttcac
    11101 cacatcactt tacattgact gaggaagatg tattggagtt tggaacaaat gcaaaaatga
    11161 atccacctct aagaagtaaa tgggacagag ataagattat agagggatta aatgatggta
    11221 ctattgaaat aatagcaacg gaccatgcac ctcatagtaa agaagaaaaa gatagagagt
    11281 ttataaaagc tccaagtgga attattgggc ttgaaacttc tttagctctt ggaataacaa
    11341 atttggtgca taaaaatcat cttagtatga tgcagttaat agaaaaaatg tctataaatc
    11401 ctgcaaagtt gtacaattta aatattggat ttatagaaga aggggctgta gcagacattg
    11461 ttatattcaa tccagaagaa gaatggacag ttgaaagctt tgtttctaaa gctgataatt
    11521 caccgtttaa aggtaaatct ttatatggaa aggttaatta tacaatatgt aatggggaga
    11581 ttgtatataa cgcctaattt aagattattt ttttaaggcg gagtgtacaa tgattgaaag
    11641 taaaagattg tatatcagca gaatgagatt gattaatcaa aattggaagt attagtttga
    11701 acaattagaa cataacatct tggtaattta gatatagtaa ggaagagaat cctttttatg
    11761 aaagaacgca ctattatatt tggaaagatt aagttcctaa tctatcatat ataatagtgc
    11821 gttatatatt taagtatgtt ttatataaac taatctaaaa atcgaaaatc tttatacaat
    11881 ttctacttat aaattgcctt tatagggtct agtttagaag ctttaaaggc tggaatcaga
    11941 ccaaatacaa ctccagtcaa tatagtagct acaattgcat aaaataagct atttaagcta
    12001 ggtatagctt caaaacctat atatttagaa acatagttag tagcagcaaa tccaactata
    12061 gttcctaaaa ttccaccaca tactgttata aaaacagctt caactaaaaa ttgaaataat
    12121 atagacctag gttttgctcc aatagcacgt cttataccaa tttctctttg tctttccata
    12181 acagatacat acattatatt cattacacca ataccaccaa caaacattgc aactacagtt
    12241 attatagata catatttgtt tacattggaa ttaatgcttt caagataagc tgtctgttca
    12301 gttgggtctg gtgtagtata agaaccattg ataccagggt gcatctcata taatttagct
    12361 ataacattgt ttgcaacttc atttacatta tagcctttag aagaaactaa gtctaactgg
    12421 taaatttcat ttgaataaga attttgactc attaaagtgt caaaagcctt tttaggtact
    12481 agggaagtag taaattgcat atcttggtaa cctccaaaga atcctccagc ttggttttgc
    12541 tgagaatcat ctaatacccc tattatttca aatatagttc catttatgtt gataccatga
    12601 cctaaggcat cttctggatt ttcaaaaatt tcacttgtac tttgtaatgt aagtaaaatt
    12661 acttttcttt tttcatcatc taaagaaaaa tctctaccac aaattaaatt tattttagaa
    12721 tcttttttta ctggtccaac atcaacatac gtagtttttt tatcaaaaga tgcttgtgaa
    12781 gagtaaactg aatcaaggtt aaacccatct cttgaaggag ctattctttc tactccttca
    12841 acaaatgata attcttctaa atcttttgca ttaaagggtt ttaaaaaaat actcatatca
    12901 tcagtaagac ctgtgttatc ggctgattca aacgaaatag tagttttatt agggtttaca
    12961 ttgtttacag atttcttaat ttctttttga aatccattac ctatagaact aacaagtatt
    13021 actgaagtta ttcctattat aatccagagt aaagctacaa atacacgaag cttgtgacct
    13081 tttaaattgg ccaaagaatt ttttaataaa ctcatgatta cacctccgaa gtaaacacac
    13141 catctttaag tcgtataacc ttagttgcat acttagttaa atcttgatca tgtgtaacca
    13201 ttattatagt cttaccttgt ttgttgagcc ttgttaaaag ctccataatt tctctgctag
    13261 tttcactatc taatgcccca gtcggttcat cagcaaagat tatctgaggg tcatttacta
    13321 ggcaacgtgc aatggctaca cgctgttgtt gaccaccaga taattgaagt ggtttctgct
    13381 taagcttgtc caaaagccca acagaactta atttatcttt aacaatttct tctctattag
    13441 atttttttag tgctttattg taaattagag gcaactctac attttgatat acatttagag
    13501 tttctattaa attaaattgt tggaaaacaa atccaatatt aatatttcta aaaacagaac
    13561 gttcattttc actcaaatta gttacatctg ttccatcaaa tatatatctt ccttcatcaa
    13621 atacatctag aaaacctaag atattaagta aagtagtttt accactacca gacttaccca
    13681 ttatcatgac aaattctcca gattcaattt ctaaatttaa ggattttaaa acatgtaatt
    13741 catctttccc tactttgtaa tatttttgaa tattttctaa tttaattaac atatgtaaat
    13801 taattcacat ccatagtaac ttcttctact tttttgtttg gagtagtatc tccattatta
    13861 gaaccagcag ttacgccatc gccttgaact gggtctcctt ctttcatttc tttagtaggg
    13921 tgttttatta ttatatcgcc ttgttcaaga ccgcctctaa ctacagcaaa atcatcattt
    13981 tgacttgcta catctacaat ttgctttttc aatataccat ctaaatcctt aaatacatat
    14041 gagtgtttgt tgtctttaag tacacaagat gctggtattt taaagcttga attagctact
    14101 tcaagtgatg cttgaagatg gaatccattt actaatcctt cttgattatc aaatgctatg
    14161 tttatatcat aataagatag tgtattctgc tgagctccca tttcagtatt tggagtagat
    14221 ggtttatcag atataaaact tattctacca gtaagctttt ggtcagttga aaaaactagt
    14281 atattgacca tttggtcaat cttcatttta ggtaaatctt gttcacttgc ttgtcctttc
    14341 atataaaact ctagaccttg aatagtcata aatgaaactg gttggtctgg atttccagtt
    14401 tggtcattca agtatactgt gccatcaaaa ggagcagtaa ctctgtcata agctttttta
    14461 tccagagaag ctatctgaga gtttaaagca gttatctgtg catttataga agcaatagca
    14521 tctttatttt cagcaatatc agaaagtgat attttttgtt ttttgtattg actaagttgt
    14581 gattttaaac tatctatttc tgctattata gttggatttt tagcagtaaa aagaaggtct
    14641 ccttgattta ctttttgacc atttgtaaca ttaacatcac ttaactcata atctccagat
    14701 atattgaagt ccttagtttg cttaggaaca accatcccat ttataaatat tttttcattt
    14761 tcaggaatag tatatgtttc gataaaattt tcattagctt gtaattcagt gtttttcgaa
    14821 tcaacataag caagaactcc tagaattaaa agtatgatta ctgctattcc tgcatatatt
    14881 ttgaatctct tatttttaaa gggattgttt ttaaaacctt tatttttaga cttgaagcca
    14941 ctacttccaa aactgctatt tctaaagttg ttacttttaa aactgttatt tctaaatctt
    15001 ttatttttaa atttttttag aaaagacatg tcttcctcct ttttataaaa actacttaat
    15061 tatatactgg gatgtacata tagaattact ataagaagtt gaatataagt taatgaaaat
    15121 aaactatcaa taataagata ctataaatca catttgaatg taatagtaaa ttttgtatat
    15181 tggttgtact ctgaatctac atcaatttta tgaccaagct tatcacaaag ttcttttact
    15241 aaataaagcc caagacctgt agatttagaa ttgtttcttc cattattacc tgtaaatccc
    15301 ttattgaata ttcgatttaa atcttccttt tttataccac aaccagtatc ttccactctt
    15361 aattgtaaat aatttttggt tcttttgcaa tagatagata tattatctcc taatgaggag
    15421 tattttaggg aattactcaa taattgctcg aatatgaagt atagccattt ttcatcagat
    15481 aaaaccttaa aattcaaatt atttaagtct aaaataattt tttttgaaat aaaatatttt
    15541 gagtattttt ttataatatt tctaatgatt ttttcaatat ttacctcttg aattaaaaaa
    15601 tccttatgaa aatcttcgag tcttgataaa tataaaattt tttcaacgga gttttcaatt
    15661 ctaaatattt cactatctat gtcatctgaa attttttcat taattaaatt ttcattttgt
    15721 tcaagaatga gttttattat agctataggt atctttacat cgtgtaccca catatttaaa
    15781 atctctttgt tttcttcaaa tttctcgata ttattattta ttatgttatt acattgatat
    15841 tgatgatttt taataatgtt caagtatatt ttttcttcat ttttacatct agctaattct
    15901 atatcattca tattaatttt gaatgtatca ttttttatct tatctaaaaa tttgttcttc
    15961 ttgatgtagc taataaatag atataaaaat aaaaacacaa catttacaac taatatatat
    16021 aaaatagtat cagtcagaaa aacatctaca ggagaaaata taataactga tactgtaaaa
    16081 attaagaaag taaaattgta tactaaaaaa cctagtttat ccttgagaaa atctttaaag
    16141 ctcataaatt cacctactta gaattgtttt aactgattat ataacccaat cctctttttg
    16201 tttctataaa atcttctaaa ccaatttcat ttaattttga gcgtattcta tttacattga
    16261 cagttaatgt attatcatct acaaaccaat cattatccca taattttttc atgagtttat
    16321 tcctacttac tatctgcccc ttatatttca taagctcatg aagaattttg atttcatttt
    16381 ttgttagctc aatagtatta tcttcatagt ttattgttgc agttgataaa tctaaagtaa
    16441 caccattgtg aataatctga tttgagttat tatcaacaaa attataagtt cttcttaaaa
    16501 gggcattaac tttagctact aagacatcta cagaaaaagg tttttgtata taatcatctg
    16561 cacctaaatt tacacccatt attacatcca tattagaatt tcttgaagaa ataaaaatta
    16621 taggaacttt tgaaattttt cttatttcat tacaccaatg aaaaccatca taaaatggaa
    16681 ggtttatgtc caataaaact aattctgggt tatagttttt gaactcttct attatatttt
    16741 caagattttc tatttgatga acatcatgtc cccatttagt aagacacttt gctatgatat
    16801 ttttcaatga gacgtcatct tcaactacca ttattttaaa cattcttacc tcctaattca
    16861 aaatagttat attaacataa ctatattcta cttgataaat taaaatattt cta
//
LOCUS       DAELSY010000084        16741 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.084, whole genome shotgun sequence.
ACCESSION   DAELSY010000084 DAELSY010000000
VERSION     DAELSY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16741
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.084"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            254..1627
                     /locus_tag="KPG09_002421"
     CDS             254..1627
                     /locus_tag="KPG09_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0967024.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILANSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIEKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDILLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEGKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            1883..2950
                     /locus_tag="KPG09_002422"
     CDS             1883..2950
                     /locus_tag="KPG09_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBG0967025.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGDVLIEMEHPQQIIPEVE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            3047..3355
                     /locus_tag="KPG09_002423"
     CDS             3047..3355
                     /locus_tag="KPG09_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBG0967026.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKVNPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3348..4778
                     /locus_tag="KPG09_002424"
     CDS             3348..4778
                     /locus_tag="KPG09_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBG0967027.1"
                     /translation="MNNSTSIKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGAKIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            4856..5230
                     /locus_tag="KPG09_002425"
     CDS             4856..5230
                     /locus_tag="KPG09_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBG0967028.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWNLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5224..7428
                     /locus_tag="KPG09_002426"
     CDS             5224..7428
                     /locus_tag="KPG09_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG0967029.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLRIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7428..8837
                     /locus_tag="KPG09_002427"
     CDS             7428..8837
                     /locus_tag="KPG09_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBG0967030.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELYIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8822..9886
                     /locus_tag="KPG09_002428"
     CDS             8822..9886
                     /locus_tag="KPG09_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBG0967031.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERSIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKKLQDINLPKILIRIPMLSELEEVINYCDISFNSELETI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            9916..11337
                     /gene="nhaC"
                     /locus_tag="KPG09_002429"
     CDS             9916..11337
                     /gene="nhaC"
                     /locus_tag="KPG09_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG0967032.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            11766..12230
                     /locus_tag="KPG09_002430"
     CDS             11766..12230
                     /locus_tag="KPG09_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG0967033.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12528..12890
                     /locus_tag="KPG09_002431"
     CDS             12528..12890
                     /locus_tag="KPG09_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0967034.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            13485..14345
                     /locus_tag="KPG09_002432"
     CDS             13485..14345
                     /locus_tag="KPG09_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0967035.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIIANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            14603..16717
                     /locus_tag="KPG09_002433"
     CDS             14603..16717
                     /locus_tag="KPG09_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0967036.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEHDVELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYVGTLTQLISM"
BASE COUNT         6631 a         1837 c         3135 g         5138 t
ORIGIN      
        1 ttttaataaa atcaataata tataaaacta ttggcatatt taatgacaat tcaaatattg
       61 tatataacca taatgaaaga tatataaata aaattttaaa tatacttaaa aatatattaa
      121 tataatgtac ataaaaaata tcataaatat atgataagat aaatacaata gcaatatttt
      181 attaattttt tgggttttta tatatttagg tactggggtg atatagttga accaagagat
      241 attttttaaa ataatgaatg aaatattgga aaatattgat gaaggggtac attttgttaa
      301 taaagacggg aaaactataa tctacaataa ttctgtacaa aaaatggaaa aaatgaacag
      361 acacgatatt ttagccaatt cattttttga tatagtcaac aaaatgaaaa taaacaggag
      421 tacactatta gaagttttgg aaaaaaggga agttatcaaa gacaatattc aaaaatatct
      481 ggacaaaaat ggaaaagaga taacagcaat taatactaca ataccaattg atattaaagg
      541 cgagtttgta ggggcacttg aaatatcaaa gaatatgaca acgatagaaa atttactaaa
      601 cagaaattat aaaaaaaatt tagtcaataa agttagtagt agacaaaaaa agggttatga
      661 atttgatgat attttagggg aaagtaattt gattaaagaa gcaatagaaa aatctaagaa
      721 agcttgcaag tctgatgctt cagtatttat atacggtgaa actggaacgg gtaaagagtt
      781 atttagccag tccattcatt atggaagtaa taggagaaac tatccattta tagcacaaaa
      841 ttgtgcggct ttgccggaat cactattcga aggtatcctt tttggaacta gcaagggagg
      901 atttacagga gcaatagata ggccaggctt atttgaacaa gctaacaaag ggacattgtt
      961 attggatgag ataaattcca tgcctattat gttacaggct aaactattaa gggttttaca
     1021 agaaggttat gtaagaaggg ttggaggaac taaggatata cctatagatg taagaattat
     1081 tgcaacgaca aatgaaagtc caaaggtaat tttaaacgag cataaaatgc gaaaagattt
     1141 atattatagg cttaacataa ttcatataaa aattcctcct cttagagata gaaaagagga
     1201 catacttcta ttgtgcaaaa aatttatttc aaagtataat aaaaagttaa ataaaaaagt
     1261 ggtaggaatt tcagatgaag ctattaaaat tttaaaaaat tacgattggc aaggtaacat
     1321 aagagagcta gaaaacataa tttattgtac tatgagtatg atggaagatg aggtacaaat
     1381 agaaacaagt atgattaatc taaacgacta ttacaatctt aaagagaata aagagagtaa
     1441 caattattct ttaaacgatt tggagggcaa aacacttagt aagattatca gtgagataga
     1501 agaaaagtat atatatgaat cattagaaaa ggcatcgtac aatataacta aagctgcatc
     1561 aattttaggt atgaataggc aaaatttaca gtataaaata aaaaaatata atatcaaaat
     1621 attataaata agcaagataa aaaaacaaaa tagaagtaaa ataaaaaagc aaaatatagg
     1681 agcaaaatta ttataaaaga gcaaaatatt ttgctctttt ttttatttct cataagaaat
     1741 attatttttt atttatgaaa atacataata aaataattat agtttcaacg ttccaataaa
     1801 tatttacttt aattttaaag ttggcacgat ttatgcttta actaattatt gagaatatat
     1861 aaatttttag gagggtgtat atatgagaaa agtaagagta ggaatatggg gatttggagc
     1921 tatgggaata ggtatggcta atatgattct aaagaaagaa ggtatagaaa tagtatctgt
     1981 ttgtagtaga agtacttcag ggaaaagtat gtatgatgtt ttaggaattg aaagaggaga
     2041 aagaccagaa gttattatca ataaaaatta tgaagaagtt tttagagaaa aaagtgtaga
     2101 tgttgttttg ttagcaactg attcatttac taaaaaggct tttgataaaa taattttctt
     2161 attaaataga aaaataaatg ttatatcaac agcagaacaa atggcatatc cacaagctga
     2221 tgatgcagat ttagctaaaa aaatggatga agttgcaaag gaaaatggag taagtatact
     2281 tggaacagga ataaatccag gatttgtatt agatttacta gtattggcac tttcaggtac
     2341 ttgtgaagaa gttactagta taaaagctaa gagagtaaat gatttatctc catttggaaa
     2401 atctgttatg gaggaacaag gtgttggagt aacaagggaa gaatttataa aaggtgtaga
     2461 agataagact atagcaggtc atgtaggttt tgtagaatca ataaatatga tagctgatgg
     2521 attaggttgg aaattagata agatagaaca aacaaaagag ccaataatga ctacagtaga
     2581 tagaaaatct aaatacggag aggcattggc tggaaatgtt gctggttgta gacaatgtgg
     2641 atatggatat gtaaatggag acgttttaat tgaaatggag catccacaac agataatacc
     2701 agaagtagaa ggtattaaaa caggagacta tgtatcaata aaaggtatac caaacattga
     2761 tttacaaata aatcctgaaa tacctggagg agtaggtact tatgcaatga tagtaaactc
     2821 tattccatta ataataaatg ctagaccagg tcttaaaact atgttggata taccagtgcc
     2881 tagagcaata atgggagaca taagaaatca gatagaagtt gaattggaag aagaatcaaa
     2941 ggctaattag taagataaca gttagctaat tggtaaaata atgtttaatt taaaataaca
     3001 gtaaattaat tagtaaggaa tagtcaatta ataaggggat gaaaaaatgg atgctaaaat
     3061 taatgattgg gttattatac acaatatagt attgactcca gaagaaagag ctccacaagt
     3121 tccagaggat actaagaaag tatccttaga aatgtgggtt aaaggtttta tacaaagtga
     3181 tgcttctata ggcgacttag tagaagttaa aacaattaca ggtagacttg taaaaggtaa
     3241 tttacttaaa gtaaatccat attatactca tgattatggt aaatgtatac cagagttact
     3301 ccaaatagga atccaagcta aagaaatatt atttggaggt gtatataatg aataattcaa
     3361 catcaataaa agatatgagt tatcaagctg ttatgggaag aaataacgaa ataatgaaaa
     3421 atgcaatcgg attggattat tcttcttttg aacaagaggg aatagggttt gattatgaga
     3481 aaatgatgag tgaaacagga tatacattac aagatattga agcaattcaa tcacagtatg
     3541 ctgttggaaa tactcctcta atagagctta aaaatttgac taaattagct agaaaatgtg
     3601 ctaaagaagg taaaggagct aaaatatttg taaaagatga agcaatgaat gcatctggta
     3661 gttttaaagc aagaagagca gctactgcag tatatcatgc caaacaaatg ggatataaag
     3721 gtgtaatagc agctaccagt ggtaactatg gtgcagctgt agcatcacaa gcggcaatgc
     3781 agggattaaa atgtataata gttcaagaat gttatgattc taatggtgta ggtcaacctg
     3841 aaattataga aaaagcacga aaatgtgagg catatggagc agaagttgtt cagttaactg
     3901 ttggaccaga gttattttat acgttcttag ttttattaga agaaacaggc tattttaacg
     3961 catcattata ttcaccattt ggaattgcag gtgtagaaac tttaggttat gagttagcta
     4021 tccaatttag agaaaagtat aaaaaagacc ctgatatagt tgtttgtaca aatgcaggtg
     4081 gtggtaatct tactggaact gcaagaggat tgataaaagc tggttccatt aatactaaag
     4141 tagtaggagc aagtgtagat ttaaaaggat tacacatggc tagtgataat caatttaata
     4201 aaaaatcatt tacaacagga catacaggtt ttgggatacc atactgtaca tggcctgata
     4261 ggtcagatgt acctaggtct gcagctagac cacttagata tatggataga tatgtattag
     4321 taaaacaagg agaagttttt tatacaactg aattattggc acagctagaa ggtatagaaa
     4381 gaggtccagc aggaaataca tctttagcag cagcattctc tttagctcaa gagttagatg
     4441 aggataaagt tatagtggta caagaaacag aatatacagg cgcaggtaaa catatatgtc
     4501 cacagcttac ttttgcaaga gaaaatggaa tagatataaa atttggaaat ccaagagaag
     4561 aaattgcagg agtaaactta atattaccag aaagaccaga attattgaaa tgtgttgata
     4621 ttgatatgaa taaaataaga aagtcattta taaaaaattg catatctaat aatcacattg
     4681 ataatattga taagctaagt aatcgagata tagaattttt aatggaagaa gtaaaaagtt
     4741 caagagattt tgttgttgat gtattaaata atttatagta attattgttt ttttatttaa
     4801 tataaaataa aaattcggaa aattctatta aatcaatgcg aatggaggaa tgttcatgaa
     4861 aaaaagagag gatgattttg aagttagaag aaaacatctt caagaactga gtgatgatga
     4921 gttaaaagaa aggttttgga atttagctac ccagatagta gaaccaatga tagaacttgg
     4981 taaaaagaat acaacaccat caatagaaag gtctgtcttg ttgagaatgg gattttcttc
     5041 tcttgaagta aaacctattt tagaaggggt tatggaaaga ggtcttatag gtaaaggagc
     5101 agggcatgta gtttataaat tggctaaatc taaaaatata acggttagag aagcaggttt
     5161 gttattagtt aagggtgaat attgggatga agttacatgt ttatttagag aaggggtaga
     5221 atcatgttaa aggaaaataa aaaattggat atagactata tattaaaaga tttggataaa
     5281 tataaaccaa aacgaagagg ttgggtttgg agagaacact tggaaaatct tgaaatggga
     5341 ccttttaaat ataaagactg tacaaaacct ttaaagaaga gtgtagggct accatcatct
     5401 aagtatttca ataatataga ccctcaacca agtccagtta ttactactga aatagcttct
     5461 ggtagatttg aggatgatat aagaagaatg agaatggcag ctcatcatgg agctgaccat
     5521 ctaatggtta tcagaactgc tggacaatca cattttgatg ggcttataga aggaactcca
     5581 caaggtatag gtggagttcc aataacaaga aaacaagtaa gagctcaaag gaaggctctt
     5641 gattttatag aagaagaagt tggtagacct attaattacc attcatatgt aagtggtgtt
     5701 gctggtcctg aagtagcagt aatgtttgct gaggaaggag tcaatggagc acatcaagac
     5761 ccacaatata atgttctgta taggaatata aacatggtaa ggtcttttgt agatgcttgt
     5821 gaggcaaaga aaataatggc ttttgccaat atagctcaaa ttgatggagc acataatgct
     5881 aatgctactg ctagagaagc atggaaggta atgcctgaac ttatggttca acacgcttta
     5941 aactctatat tctcagaaaa aataggtata gataaaagta atatatgctt atcaacagtt
     6001 ccaccaacag cacctccagc accatgttta agaatagact taccttacgc tgttgcactt
     6061 agagaattat ttagtgatta taagatgaga gctcaaatga atactaaata tatggaatca
     6121 tcaactagag aagcaacagt tactcatgta ttaaacttat taacatcagt attaactaga
     6181 gctgacatac aatcaactat aacacctgat gaaggtagaa atgtaccttg gcatatatac
     6241 aatatagaag catgtgatac agcaaagcaa gctttagttg gtatggatgg gcttatggat
     6301 atgatagaac ttaaagatgt cggtgaatta agagacaaag caagagaact taaagaaaga
     6361 gctgtattat atatggaaga aatatctgaa gttggtggat attttgaatc tgtggaacaa
     6421 ggattttttg ttgattctgg aaattatcca gaaagaaatg gtgatggaat atctagaaaa
     6481 ataaaaggtg gagttggtga aggtactgtt tatgaaagag aagaagatta tttagcacct
     6541 gtaactgctc actttggata caataatgta gctcaatatg atgaaaatgc tatagataat
     6601 ccatcaatgt taatagatgg atgtacattt gaaaatccag acaaaataat atatattgat
     6661 gagcttgacg attttgataa tgtagaaaat agactaaaag aatcatatga gtacagaaat
     6721 ggaactaaaa ttaagcctga aatggaatgg tgtgcagatg gaattgtaat gataacaatg
     6781 atgcttccta ctgataaaag aacagcagag tttgcagctt tggaatttgt caaaaaaatg
     6841 aacttacaag aaattgaggt cataagtaga gaagttatgc atgaatctga agggactaga
     6901 atagaagtga aaggaagagt tccgtttgat atagatttaa acaatttagt aataccagaa
     6961 gaacctaagg tactaactga tgaagaaata agagcagata tagaagaaaa acctatgaaa
     7021 atagtttcag ctacggttgg agaagatgaa cattcagttg gtttaagaga aataatagat
     7081 ataaaacatg gaggtattga aaaatatggc atagaatgcc actatctagg gacttctgta
     7141 cctgtagaaa aattggtaga tgcagcgatt gaactgaatg cagatgcaat acttgcatct
     7201 acaataataa gtcatgatga tattcactat aaaaacatga aaaaattaca cgattattgt
     7261 gtagaaaaag gtataagaga taaagtaatg attgcgtgtg gaggaacaca agtaacacct
     7321 gaaatagctg ttgaacaagg catcgatgct ggatttggaa gaaacagcaa aggcatacat
     7381 gtagccactt tcttagttga aaaaagaaga gaaatgagta ataaataatg gaaaatagca
     7441 aagtttcttt aaaaatagat gtgttggtag ctgaaattgg aagtactact acagtggtaa
     7501 acgctttcca cgatattaat actaacaatc ctatattttt gggtcaaggt caagctccaa
     7561 caacagtgtt tgaaggtggc gatgtaagaa atggcttatc aggtgctatt aaagatttag
     7621 ctaataaatt atctgtagat gatatagagt ataatgatat gtttgcaact tctagtgcag
     7681 ctggtggatt aaaaatgact gttcatggat tagtctatga tatgacagta aaagcagcta
     7741 aagaagcagc tttaggggct ggagctgtta ttcgtcaaat aacatcaggt agaattaaaa
     7801 gaactgattt aaataaaata aaagaaataa acccaaacat aattttaata gctggtggag
     7861 tcgattatgg agaaagagat acagctattt ataatgcaga aatgatagct tctatgaatc
     7921 taggcatacc agttatatat gctggtaatg ttgaaaatca agaggaaata aggttaatat
     7981 ttgaagatac taattataag ctttatataa ctgaaaatgt ataccctaaa atagatttat
     8041 tgaatataga acctacaaga agaatcattc aaagtgtatt tgaagaacac ataactacag
     8101 caccaggaat gaaatacatt aaagaaatgg ttaatgaaaa tattactcca accccaggag
     8161 ctgtaatgga agcttctaaa cttttatata aaaatattgg agatttatta actttggatg
     8221 taggtggagc aactactgat gtacattctg ttacagatgg aagtgactat attaataaaa
     8281 ttttggttaa tcctgaacct actgcaaaga gaagtgttga gggggattta ggtgtatatg
     8341 taaatatgaa gaatatagta gaagttatag gtaaagaaaa cttacaaagt gaactatata
     8401 tagatattga tgcagtaata gagaattatc ctccaatacc aaaatcaaaa gaagaaatat
     8461 tatttgttga gaggttgaca aaagaagctg ttgttaaagc tatgctaaga cattcaggaa
     8521 aaataagaaa tatctatgga acaactggaa aagtcaaaat tgcagaaggg aaagatttaa
     8581 cagaagtaag atatatagtt ggtacagggg gagcacttac taggctccct agtagaattg
     8641 aaatattaga taatatgctc aaatacaata aaaataatga attactattt ccaaaggaaa
     8701 aaacaaagat attaatagat aatgactata ttatggcatc tatgggtgtt ttatctaaaa
     8761 aatatgaaga agcatctctt aagctcctgt taaaaagttt gaattttgag gaggaaagat
     8821 tatgtatcct aggttagaaa tagatataga aaagttaaag cataatgcaa aattaatttc
     8881 gcagatgtgt catgaaagaa gtattaagat atcttttgtt actaaatcat tttgtgcaca
     8941 aaaagaaata gtagaagaaa tatgtagtga aggaatagac catatagcag attcaagaat
     9001 acaaaactta aaaaaattac aagatataaa tctacctaag atattgatta gaatcccaat
     9061 gttaagtgaa ttagaagaag ttatcaatta ttgcgatatt agttttaact cagaattaga
     9121 aacaataaag aaattaaatg aattatgtga aagtaaaaat attattcata agatagttct
     9181 tatgtttgac ttaggcgatt tacgagaagg atacttttat gaagaagact tttttaataa
     9241 tgtaagagag atagtaagat taaaaaatat tgaaataatt ggtatagcta caaacttaac
     9301 ctgttatgga gctattatac catctaggga aaatataggc agactggtaa gtatagcaaa
     9361 aagaatggaa gaaaaatttg gaataaatct agaaatagta tctggaggta attcaagttc
     9421 tattcatctg ttgataaata ataatatgcc agaaggtatt actaatttga gaataggcga
     9481 gtccattcta cttggtagag aaacggcata cggtgaaaat ataaatggaa catatcaaga
     9541 tgcatttaaa ttaatatgcc aagtagttga atgtaaagaa aaaccatcag ttcctattgg
     9601 agaaatagga gttgatgcct ttagaaataa acctgtttat gaagataagg gaatcttaaa
     9661 aagggcaatc atagctatag gtaaacaaga tataaatatt gactctttaa ttccaataga
     9721 cactgatata aaaatattag gggcaagttc agaccacatg atactagatg taagtaacac
     9781 aaaatatgat tataaattag gtgacaattt agaattttta ttaacttatg ggggtatcat
     9841 gtcatctagt acaagcaaat atgtttcaaa aaaaattata gtgtagtgtt taaataatta
     9901 ggaggaaact attttatgaa agagcgagaa tgtagaaaag caagtctgtt tgatgcaatg
     9961 ataccaatta cttgtttgat attattttta agtctaggag tattagtgta tggttcatca
    10021 cctcatgtac cactaatagg agcagcagca gtagcaggac ttgtggctgt atatagactt
    10081 ggatttaaat ggaaagagtt agaattaagt atgtttaata gtataaagat ggcaatgcaa
    10141 gcaatcttaa tcataattat aataggcgta cttataggaa cttggatagt aagtggagta
    10201 gttccatgta tgatttactg gggactaaaa attttatcac caaatatttt tttagtagca
    10261 tcaacattag tctgtgcaat agtatctctt gcgacaggtt catcttggag cacaatgggt
    10321 acagtaggag tagctctttt aggaattggt caaagtttag gtatgccaat tggattaatc
    10381 gtaggttcta taatatctgg agcttacttt ggagataaac tatcaccttt atctgaaacg
    10441 actaacttag ctccagcaat ggcaggaaca gatttgttta cacatataaa atatatgtta
    10501 tattcgacta taccatcatt attaatttgt ttagtaattt atggtgtaat tggaatgaag
    10561 tattcaggtc aagcactaga tataaaacaa atagaattaa ttaggtctac attagattct
    10621 acatttaata ctttatcacc agtattatta ttagctccag caatagtaat tggattagtt
    10681 gtatttaaag ttcctgctat accagggctt atagtaggtg ctgtattggg agtattgttt
    10741 gcagtagttt ttcaaggaga atctatgaat acgatattag atgcagcaca aaatggatat
    10801 gtttcgtcaa cacaaataca ggaggtagat gctttattat ctaaaggtgg gattatgaat
    10861 atgatgtcca ctgtatcctt aacaatatgc gctttatctc ttggaggtat tttagaaaaa
    10921 acaggtatgc ttgaagtagt agcatcttct ttactaagat tagcaaaggg tgtatttgga
    10981 acagtattat gtactatggt aacttgtaca attaccaata taatagcagg tgaacaatat
    11041 ctatctatag ttataccagg aagaatgtat aataaggaat ataaaaagag aggtatacat
    11101 cctaagatgt tgtcgagagc tttagaagat agcggaactt taacttctcc attagtacca
    11161 tggaatacat gcggggcgta tataacagca acattaggtg tatctgcact aacatatggt
    11221 ccatacgctt tattaaatat tataaatcca atagtatctt tagtattaat tgcatgtaag
    11281 tttaaaatag caagaataga agatgaacct gaaacttgtt taaattttga tgtttgataa
    11341 tagataactt atgtaagaac gtaggaacta gggggggagc ctgtggggaa ttagtaagac
    11401 atattttagt gtgcgaaatt ctataaatca gtaatcttgt gactttagcc atgggaggtt
    11461 cagattagta aatttatagt tttatattag tctatacttt gatcttttaa ttgtttgtat
    11521 aaatttttta tagaaaaaga tttcttttag acttgcctaa aaattaggca agtcttttaa
    11581 gtatgttctg attataagag cactgtgtga caagaatgat gagattattt ttgctatact
    11641 ttcgatataa gaaatggaag gaggttatgt tttatattta tatgaaataa ttagttacac
    11701 agtgaatttt cgtataattt ggtataaaaa gattagaaat gaaaataaag taatggagga
    11761 ataaaatgac tagaatatcc aatataacaa tagctgaaca atctgagtac tgttttttgg
    11821 caatacgaaa aacaattgac tttatggtag agttttcaga gttttcaaaa caaagttttg
    11881 aaaaaatttc taaatatctg gaggagagag gaattttatc ttcaggagca ccgattgtat
    11941 gttttcataa catggattta gcaaaattgg atgttgaagt agggtttcct gtagccactt
    12001 atatagatgg gaaaaatgag attctacaaa ggattgttcc tgcacagaaa ataataactg
    12061 caattgattt aggaccttat gaattacaag accctacttt agaagactta ttttcatggg
    12121 caaatagcaa tggatataaa cttcatggcg atatatatta ccagtattta aatgatatca
    12181 atcgtccagc tagtgaatat cttacgaaaa tgatgttacc cattatatag aaagttaaag
    12241 attatcgttt gttagttgat aattagaaga acatttaatg ctactgcata aattttatcc
    12301 taaataatat caaggtgatt taatttatgt attctaatgt aactgtagat aatcaattta
    12361 taactaactt agtgtaattt agatttaatt tattattcta atgtaacccc aatatattgt
    12421 taaggtctac ttaagatgct caaaaggcta tgcttaattt attgttatat aatttgtaga
    12481 gtggtataat ttagaagtga tactatatag atatataggg ggaaaatatg aaaatatatg
    12541 atatattaac acgtttttat attcaagata tagaaaaagc catacctttt tatgagaatc
    12601 ttttaaaaga aaaatgttct ttgcgttttt cttataaaga agttgggttg gaattagcgc
    12661 aaataggaaa tgttttacta ttatctggtt ctgatgatgc tcttaaacca tttatagaaa
    12721 caaaatctac ttttatggtt gattctgttg atgaatggag aagttatcta ttggataatg
    12781 gagcagttgt agtaagagat aagaaaaaag ttcctacagg atataatatg acattgagac
    12841 atccagatgg tacaattata gaatatgttc aacatacaaa gcaggattag ttaaaaatag
    12901 attttactac tgagcaacta attagaatct attcttagac gtacaattta ttatttttgg
    12961 tattaataga ttataagtca aattataata tttcaaaata gaatagaaat ttatttacat
    13021 aaaagagagt gtttaaaaat gaacttttta gttcacgaag cactcctttt tgtatgaaat
    13081 caaatacatc aatatataaa acaaactgat gaagagctta aattaaaata aaaagattca
    13141 taacaggacc ccattaaact ttaagatagg gtaagtttgt aatcatatta aataattgta
    13201 aaaagctgtt tttagtcgta taatataaat atttatcatt taattaagga atctaaaagg
    13261 aatctaataa gaagaaaata ataaataact ttttagattt actaaatgaa aaattattga
    13321 aaattaaagg attagagtct ttaatcagaa agtaaaaagt aatatattaa atttatgaat
    13381 tgaaaactta aacaacaaat ttgtttataa aatagcaaga ttcattaaat ataactaagt
    13441 aataattgat ataatgttgc atggataaaa ggagggaagc tgcaatgtct tacaaagtaa
    13501 ttaatgtcat aactgctatt gactatatag aggagcattt atcagaaaag ctggatttag
    13561 atataattgc taatgctgtt cattactcaa aatatcacct gcatagaact ttcacaactt
    13621 ctgttggtct aacaatgcat gattacataa agcggagaaa gttgacagaa gcagcaaaat
    13681 tattggtatt ttcaaagaaa ccaattattg aaattgcatt gatagctgga tatgaaagtc
    13741 aacaggcatt cacttctata tttaaagcta tgtataaaaa atctccaaat aaatatagga
    13801 aagaacaaga attttatcct ttgcagctaa gatttgtttt gaaaaaagat aacctatttt
    13861 caaagaatgt actagagttg gaaaaagaaa ttaaacttgc aagtatgtct gacataccat
    13921 tatggatgaa tttagtgaga ttagtcatag atggatttcc aaatttgcaa gaggaagaat
    13981 atatatatca attagaacag tatattttag aagaaagagc tttaatatta aaacttaata
    14041 atgtcgctat tgcaaatatg gtgtttaata gagaaactag aagtattgat ttttttggaa
    14101 tacatccaca gtatcgtaat tcagacattg cacaagtgtt tttgaagaaa gttattgaag
    14161 atttcttgat agatacagat atctcaatta ctacctttcg agagggtgac aaagctgata
    14221 caggacatag agatatgata aagaagttgg ggtttgcaga agcagagttg cttgtggaat
    14281 ttggatatcc tactcaaaaa tttattcttc cttgtgtaga agaaagagat aatagacaaa
    14341 attaattgta gattaaatgc aaagtttacc acatattttt tgtttgaatt attttatata
    14401 taagagtatt tctgctttta atgtattttg cttgtaagtg tacttatggt aataaatata
    14461 gaaagattta gttttttaaa aaaagaactt taaagctaaa aagctaacat ttaagttaaa
    14521 tagcaagaat gattaaaaac tccaaaacac aattaagtat aatgattcag tgtaagtact
    14581 taattaacaa aggaggattt aaatgtctga aaataaacaa gtagtaaatt gcgatattat
    14641 tgcatccaat tctaaacata atatgtgtga acatgatgta gagttattaa ctttttttcc
    14701 agatgagatt gcacatctaa tagagataaa taacaagcta gacaatgctt ttaagaaagc
    14761 tgaaaatctt gttgacaagc ttgataagga ttatatggac gctaaaatgt atatggtaaa
    14821 aaatcgtggt gaaatagacc cacatgaaat gtttcaaaat gaacaaggac taaaacaaat
    14881 agataactat ggggctttta tggtaaaagt tcgagataag attaataaaa tcaaagattc
    14941 tccatacttt gctagaatag attttaggct gaaagatatg gatgatgaat ctaagtatta
    15001 tattggtaga tttgcatttg attatgaaga tgagcttata atacttgact ggcgttctcc
    15061 aattgccagt atgttttatg attatgaaat cggaaaagct ggatatgatg ctccaatagg
    15121 atgggtggat ggagaaataa caagaaaacg acaatttaaa attaaaaatg gaaaattgga
    15181 atatgcacta gaaagttcca ttaatataca agatgatatt cttcaaaaag aacttagtca
    15241 cacttcagat gaaaaaatga agtcaatcat ttcaacaata caaaaggaac aaaatcaaat
    15301 tattagaaat gataaagctg atacactgat tattcaaggg gtagctggtt ctgggaaaac
    15361 atctatagca ttacacagaa ttgcttttct tctttatcga tttaaagata aaatatcagc
    15421 aaataatgta attatcctat cacctaataa agtctttgga gactatattt ccaatgtcct
    15481 tcctgagctt ggagaagaac cattatgtga attaagtttt gaaaatatag cagaggtaca
    15541 attagataga gttattaatt ttgaaagtga gaaagaccca ttagaaataa atgatgctaa
    15601 atggtctgaa agagtgcgtt ttaaatctac acttgatttt gtaaaactta ttgatgatta
    15661 tataaaacaa atgccaaata agatttttat tccaaaagac tacacttttg gaagttttac
    15721 agctaaaagt gactggatac agagtagatt tgaggcatat aacagatatc cagttaagaa
    15781 gagattagaa aaggtagctg aagacattca ttataaattt gaatcagata atattatgga
    15841 agaagattta ccaaaggtaa aaagtatact taaaagtttg aatggaatgt tgacaataaa
    15901 aaatactctt acactatata aggatttctt taaacaaatg aatatatcaa acatgtttgt
    15961 gatggcagaa aagaaaacac ttgaatggtc agatgtatac ccatttatat atattcatgc
    16021 tgcatatgaa ggtatacaag aagataagat tataaggcat attgttattg atgaaatgca
    16081 ggactataca cctattcaat atgctgtaat aaacttactt tttaagtgta aaaagactat
    16141 tcttggagat tttggacaat tggttaatcc taatcatact catacattag atgatatgaa
    16201 acagctatat aatgatgggg aactagttat gttgaacaag agttaccgtt ctacttttga
    16261 gattattaat ttcgccaaaa agattcaaga tatatcttca ttagagccta ttgaacgaca
    16321 tggtgaagaa ccggctttat taaaatgtaa taacgagcaa gatgaaataa ataagataaa
    16381 aatagaaatt gaagaattta aaaagagtga taatgcgact cttggtatta tattaaagac
    16441 agataatgac gctaaagctg tatatgatgc gttaagagaa gaatatagtg ttcacttaat
    16501 atcttctgaa agttctagct ttacaaaagg agtatccata acttctatta aaatgtcaaa
    16561 aggtttagag tttgatgaag ttattgtacc ttcagtaaat aataaaacat attatagtga
    16621 ttatgaccgc agtttgttat atattgcttg tacacgagct atgcacaaac ttaagttaac
    16681 atatgttggt acattaactc aacttataag catgtagatt atatcttata tgcatttatt
    16741 a
//
LOCUS       DAELSY010000085        16532 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.085, whole genome shotgun sequence.
ACCESSION   DAELSY010000085 DAELSY010000000
VERSION     DAELSY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16532
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.085"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(145..2283)
                     /gene="vanT"
                     /locus_tag="KPG09_002434"
     CDS             complement(145..2283)
                     /gene="vanT"
                     /locus_tag="KPG09_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG0967037.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(2301..3107)
                     /locus_tag="KPG09_002435"
     CDS             complement(2301..3107)
                     /locus_tag="KPG09_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0967038.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(3104..4204)
                     /gene="vanG"
                     /locus_tag="KPG09_002436"
     CDS             complement(3104..4204)
                     /gene="vanG"
                     /locus_tag="KPG09_002436"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBG0967039.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(4465..5547)
                     /locus_tag="KPG09_002437"
     CDS             complement(4465..5547)
                     /locus_tag="KPG09_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0967040.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            complement(5597..6298)
                     /gene="vanR"
                     /locus_tag="KPG09_002438"
     CDS             complement(5597..6298)
                     /gene="vanR"
                     /locus_tag="KPG09_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBG0967041.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KDLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(6732..9263)
                     /locus_tag="KPG09_002439"
     CDS             complement(6732..9263)
                     /locus_tag="KPG09_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG0967042.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVKEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(9371..9988)
                     /locus_tag="KPG09_002440"
     CDS             complement(9371..9988)
                     /locus_tag="KPG09_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBG0967043.1"
                     /translation="MCKKTYLLVVSILIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIITPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(10350..11234)
                     /locus_tag="KPG09_002441"
     CDS             complement(10350..11234)
                     /locus_tag="KPG09_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG0967044.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLVLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAVKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(11425..12675)
                     /locus_tag="KPG09_002442"
     CDS             complement(11425..12675)
                     /locus_tag="KPG09_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0967045.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFIYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDCKAI"
     gene            complement(12897..13661)
                     /locus_tag="KPG09_002443"
     CDS             complement(12897..13661)
                     /locus_tag="KPG09_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0967046.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(13655..14380)
                     /locus_tag="KPG09_002444"
     CDS             complement(13655..14380)
                     /locus_tag="KPG09_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967047.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(14382..14750)
                     /locus_tag="KPG09_002445"
     CDS             complement(14382..14750)
                     /locus_tag="KPG09_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0967048.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(14926..16470)
                     /locus_tag="KPG09_002446"
     CDS             complement(14926..16470)
                     /locus_tag="KPG09_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG0967049.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQFISKYAIQIILIMAIICMMLIFYIIYDKLNFKIKLQKIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIDLNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT         5651 a         2936 c         1650 g         6295 t
ORIGIN      
        1 aataaaaatc tgaaacatca cctttttata acttaaattg tcatgtaagt tataaaaaat
       61 atatattaga cataatatta ctaaaaatgt ctagtcagag taaaataaat tacacagtac
      121 aacaaatatt actatttaat tgagttactt ctttataaca tttaaccttc ttcctactct
      181 gctcaataat tcattcgata tactacatga gttatttgca acttcagaag cagataattc
      241 agataaatta tctcttccta ttattattgc cgtattacca acttctacat taggtatatc
      301 tgtaatatca acagcaagtt ggtccatgca aattcgaccc actattgctg ctctacaacc
      361 atttattatt acgtaacttt ttccacagga taaatttcta ggtataccat cggcatagcc
      421 aatagataat atagcaatta cgctatctct gcttgcaata aagactcttc catatccaaa
      481 actatcatct ttttgtattt ttctaataag aattacttgt gattttaagg ataaaacagg
      541 tcgtaaatca agttgcaatt tagtcgtatc atttggagaa cttaatatcc cgtataatgc
      601 aattccaatt cttacataat tacacttaag attgggataa ttaaaaaaac cataactact
      661 ttgaatatgt actttaggaa tttttatacc tttttttaac agaaaatcaa ttaacttata
      721 aaaacattca atttgtttat ttgtaaagtc tacatcttca tctttcagac tatctgaaac
      781 acacaagtgt gtaaatatgc cacatatatc taagtatttt aatttaaatg cctttaaaat
      841 tgacctcaca tcattaacat caaaacccag tctgtgcata cctgtatcaa ttttaatgtg
      901 tgattttatg ttgtatttct gtttgtttaa agagatagca tgattaaggt caataacact
      961 ctgtgtaaga ttgtatttat gaagttcctt tgcccttatt gggttagtat atccaagaat
     1021 caatatttct cctagtattc catatcgacg taaatcaatc ccctcatcta ttgttgctac
     1081 tgcaaatgct tttacaccta tttggtttgc acaagtcgct acttcaaaag cactatgacc
     1141 ataggcatta gcttttacaa ctgccatcaa ttcacaatct tctggcatag ctttttgtat
     1201 catctttaca ttatgttcca aattatttaa atcaatttca atccaagctc tattggtatt
     1261 taccttatgt tctcctttca agtctttttt tagaggttta agaatcaata taataattat
     1321 agaagctata aaagatgcta tacttaccac tatataatga ataaggctat tgtcaaccaa
     1381 gacagtttgt attccgaata ttttagaaaa catacgaata acaacaatca tcataggatg
     1441 tataatataa ataattaatg atgatgttcg caataaccta gctcgagaac cttttaaaaa
     1501 tgttaaggct gtaaataaga aatacataca tggcaataac ataatataca tgctatcatg
     1561 acgctgtacc cctagtttat gtaaaaacat tccctcacat aacataagta agaatgatat
     1621 aagaaatcca atcaagctat ttttagttga catataatta gatttattag atattattcc
     1681 accaagtatg aaaaatacag gtgcaaaaaa tataccattt cgtgtataat ctgatacttc
     1741 aaaaatatac ccatataaat tctttaatag tggtattttt tctaaaaaac cataataact
     1801 atcaccaaac ataccaatta cataaataac aatagtgatt actaatgagt actttaaacc
     1861 tagttttttt acaagaaagt atgcaatact tgcaccaata attgatgcag gtagatacca
     1921 taaatgatat aaagtcccat caaatacaat gtccttaata atattgggca ctaaattatt
     1981 catggaaaaa tatccagtgt aaatatttaa gggaatataa attacaatag ctatcccgta
     2041 aataaataat acatttttta gaaaagattt cagtttatta caattataat tatatgttga
     2101 aattagaaaa aagcctgata gcataaaaaa gaatggcaca gcaatacgag caattatact
     2161 agttaacaca aagtcagcca ttttactata tgataataat ggagatgtat gaattgcaat
     2221 tataagtaat gcagctataa tcctaaaata atcaagccct gcataagatt tattcctaat
     2281 cattcttgtt cctccatttc ttagtccttt ctatttctcc aaaatgtcac aataaaacca
     2341 tccttattgt tacccgatat ttgatataca cattgttcag gtatagagat tctcacttct
     2401 tctttagtat gaataggcac ataaaagatt tctataattt tttctttatc ttctattttt
     2461 aaacaatttg tatacgaaaa tctcttaata tattctatat attcttcaag ggcgagattc
     2521 attttattga taatctcaga atgtggatat ccgacataac gaaaatgcca tggttcttga
     2581 gatattcctg ttatttcttc ttttttacac tgataacgct ctataaaacc ataatgtagt
     2641 gctgccttac ggaactcatt acaaatacca ttataaggaa attcagggca gataaaatct
     2701 ataacctctt tcttcaaccc taaatcaata gcaagccctg tctgatgttc actgtgattt
     2761 ggaagtgcta caaacttttt agtaaagtct tttccattgt cacgcagaga gcttgaatat
     2821 atatcttgtt gttcctctgc agtacgatat ccacttacag gtactatttc atttattcca
     2881 catatacttt ttaaaatatt ttgtaaaata tttgtagctt cacgtttcat aagaatactt
     2941 ggaaaccttg tatctacagg aattaaatct atatcttcag atgttttaac tggtaaaaaa
     3001 gcatttacta gaattagatt cccatcataa actttatctt tagtcaataa gatagtattt
     3061 gtcattttat cttctttatt tttatctaat aaaatattat tcatcatttt atgtacacct
     3121 caattatctt atccaatatt tcttcaaaag acagtccaat acctttcatc atattcggat
     3181 aacgactatg agaagtaaag ccaggaattg tattgacttc attaaaaata atttcaccag
     3241 atggagttaa aaacatatct actctagcaa atccagaaca tccaagagtt ctataaataa
     3301 tttttgctgt ctcttgtatt cttttctcac aatccaaatc aattctagct ggcatatgaa
     3361 tttttgaact tttcaatgta tatttttctg tataatcaaa aaatcctttt gacaattcta
     3421 tttcatctac tcttccaaca agtaagtcat cgtttcctaa aactgcacac cctacctcaa
     3481 aaccatcaat attttcctca attattactt cattatcatg ttcaaaagcc aagtctactg
     3541 ctacttgtaa ctcttctttt ttataaattt tactaatacc aaatgatgaa cctgcacgca
     3601 caggttttat aaataatgga taagttaagt cttttgacag ttcagccaca ttaaatttat
     3661 ctgagtattt tagtaatatt gattttggta ctttaatacc ttctgaatga acaagtttat
     3721 gtgatttgtc tttgtccata cataatgctg aagaaagagt accacatcca ataacctgta
     3781 tacctgccaa ttcaaataac ccctgcactg ttccatcttc tccatttttt ccatgcaata
     3841 caggaaatac aaaatcaagt ttagtgtatt taatatcatt ttcacatgat tcaatagcac
     3901 tatttggaat atgattctga gaaacagcat caggaattaa aaattcaata ataccatttt
     3961 tgacacggtt ctgcgaaaca ataactggaa ttatatcagt atccctttca aaccaagtgt
     4021 tattctggat attattataa tctccaatat aatgatacca attaccctcc cttgtaattc
     4081 caattggtat aatatcatat ttttccgtat tcaaattctc aagtactgca aaagctgatt
     4141 gtaatgatac atcatattct gttgagtttc caccaaatat aactgcaatt tttttattat
     4201 tcatctttga ttctcctttg ttgatttatt tatttttata caatctacag aaacatgtta
     4261 aaatactttt tctatttcaa atccaatcaa ccttcactta caattgcatc tgttccacat
     4321 aatatatttt cacatttttc tattgatata attactgtat caaaataaaa gcactcttgt
     4381 tttgatacag taattatatc aataacaaga gtgctcttta tttatttatc ctaaagaatt
     4441 tcctattttt ttatacttaa attcttacaa atttcctaca atcggaataa taaccttaaa
     4501 ggtaattata ttgttctcgc tatgagctgt tattgtcccg ttatgaagct ccactatttc
     4561 tttagctata gcaagtccca acccagctct accactgttt gaattacgag atgtatccaa
     4621 cctgtaaaat tgttcaaaaa tacgctcaag tttttcctct ggaatcgtat tgctatgatt
     4681 tataaattta agctttatat aatcatcctc ctgtacagct gttatttcaa tagtagtatc
     4741 ttcaaaacta taataaacag catttcgtaa taagttatca aatacacgtt gcattttatt
     4801 aacatcacac cttatcatca tatttggtgt aacattcagt gtatatttta aattcttact
     4861 catcaaagta ggtttaaact catatgttag ttgttcaagc atacgagtta agcttactgt
     4921 actgtattca agtgatatat ttgataaatt aaaccttgta atttcaaaga attcattaat
     4981 taaatcttca agacgctctg ccttatccaa cgaaatagat aaatattttt ttcgcagttc
     5041 ttcagaaatc tgactttcat ctcgaagtag tgtcagatac cctataactg atgtaagtgg
     5101 tgtttttaaa tcatgtgcaa gataaacaat aagttcattc ttacgttgtt ctgccatttg
     5161 tgtctcaaat ttacgttgtt caagtatatg tttaattgag tttattttct tttctattgc
     5221 caataactct ggagataaag ccacttctcc aacatcttct ttgataagag agtcaatacc
     5281 ttgatttatt tcactaaaat attttgtaaa ccaatttaaa taaacacgga atattataaa
     5341 aaacacaatc accatagcta gtatcatgaa gagctccatg ctattacgga aaatctgctg
     5401 atataaattc aatgcagctt tatattccat acgaaaagca ttttcaaaga atgatactat
     5461 ccagatagaa aacttaccat acaatataaa agaatataaa atccatatga aaataatggc
     5521 tataaatgcc attaaagctg ttctcataaa aattttattt ctcacttgag taaagtcagg
     5581 attgacttta atatttttac ttttcaattg tataacctac tccccataca gtttttataa
     5641 atttcgggtt tttagaaggt tcatgaattt tttcacgtaa tcgccctata tgagccataa
     5701 ctgtgttatt attatcaaga tatttttctc cccatacagc ttcaaacaat tcttcagaag
     5761 gaaccacttt tccttgatgt tcacataaat accaaagaat agaaaattca atcggtgtta
     5821 atgctacttc tttcccaaat aaactacatt tatgtgtttc tttattgata atcaatcctt
     5881 taatatcata ctcattaaca attatcgact gttgttcata agaattatta tatcgcatat
     5941 atcttcttaa ttgtgtcttt actcttgcaa caacttccaa agggttaaat ggctttgtta
     6001 tataatcatc agcacctatg gttaaaccca ttatcttgtc tatatcttca attttagctg
     6061 ttaacataat gataggataa tagtattttt ctcgaatttt ctggcaaata tgaaatccat
     6121 caatatcagg aagcataata tctaaaatag ccatatccaa atctttagat tcaatacatt
     6181 ctagagcttc cttacctgta taaaacttgt atactgtaaa accttcattt tttaaatata
     6241 cttcaattaa atctgctatt tctttttcat catccacaat caaaatattt tcgttcatag
     6301 attatttttc actctcctta ttatcgaggt catcatttat aatattggta aatttacata
     6361 tcaaataatt acaagcatat ttgtaactaa tttttataag aaaagaacta ctacatcaga
     6421 atgtgttgat gctactattg gaacacaaaa actgagatta attgttatta taaaaactcc
     6481 cctaacacgt ccttattatc gcataacagg tattaacttt caataaaaat atctatcagt
     6541 caaacacaat tcaatttatt ataccagaga caatccatat aaaaaacaat taaacctatg
     6601 tatgtaacca cagacattac taaaatttcc atgttatttt atataaaaat tcaaaaaagg
     6661 agttgaattt aaaatttgat tagaaaaaaa tcaattttga gacaactcct ttttatttta
     6721 atgtatttaa attatagtat atcagccttc ataatatcct caaaatcacc tttttcagat
     6781 attaatctta tcaatttaac agtacagctt aaatcattta taagtatagc tccaactaat
     6841 gaaccatctt tcataaataa ttttttatat attctttgac cttcatcttt tgatgaaatt
     6901 tctttatcaa aattttgtat attaccaata ctaaatactt ttacattcat tgcatctaat
     6961 gaaactggat atacttcact ttcaaatgtc aaattatctc cacttgcatt tgctcctgct
     7021 atttttccca tttctactga agatggccat attgctaaac taatatttcc cacttgtgca
     7081 acatctccac aagcatatat atctttaaca tttgtctgta atgtcttatc tacaacaata
     7141 cctctatcaa tttttacaga tgtatcctga accatttgag tgtttgctct tacaccaatt
     7201 gaaaatacta ccatatcaca gtcaacacta tctccatctt taaatacaac tttttgagct
     7261 tttccatcac cttcaatacc atcaactgct acacccaatc ttacatctat tcctgtatct
     7321 cttacacatt gttctaatat tttactaccc tctttatcaa gttgttttga tagtatgcta
     7381 tccatggctt caactactac tacttctaat ccttttagtc taaattccca tgcagcttca
     7441 agtcccaata aaccgccacc aatcactaca actttcttag atttttctag tgcttctttt
     7501 acactatata aatcttttat atttctaaga gtaaacacat tttctaaatc atggccctta
     7561 atcggtggta taaagtttct acttcctgta gctattacaa gcttatcaaa tttaatagct
     7621 ccatcattca ctataatagt tttattcact tcatctaatt tatctacatt cgttcctaat
     7681 attactacta tattattttt atcataccaa tctttatctt ccatgtagaa atctgaacca
     7741 agttcttcat ttataccatc agaaagtgca ggtctatagt aagtcaactc atcttcatta
     7801 gaaatcatag ttatcttaca agttttattt ctttttctta tagcatctgc tgcataaaaa
     7861 ccagctgctc catttcctac tattacgaaa tattcatcag tatcttttct aaaagtaact
     7921 tcatcttctt caacttctat aaattggtct tccttagctc cacatacagg gcatacacta
     7981 ggagcactat caccttcaaa aacttcatta caaattacac atttaaattt cttagttttc
     8041 tttttacttc caaaagtaga atttaactta tctaggaatt tacctgtata agaatgtaat
     8101 ttatcaatct cttcatttga tggtttaaag ttaacagcaa aaggttttac tgctgtcatt
     8161 ctaagctgac ttattctctc catagcattt tctatacctt ctccactcca tccatatgaa
     8221 ccaaatactg aagcaacttt tccaccatga agaactgggt ttaacgatat taatatatcc
     8281 catataggct ttaacgcatc tcctaatatt gttggtgttc caaataatac accttgtgat
     8341 actgctattt ttgccaaaat atcttcttgc ttatgctcta ttacatcaaa taaatttact
     8401 ttatagttgc tatttttttc aatatatgct tttatagctt ctgccataat ctttgtatat
     8461 ccatgtgcag aaacataaca aattgtaact tctttttcta actttatttg ttcctttaca
     8521 ctccagttat aataaaggtc tataattttt ctaggatttt cagttaatac tggaccatga
     8581 cctggacata cagtatctat atctaaatct tttatctttt ctatagcagt taccatagat
     8641 ggtttaaatg gacccattat acaatcataa tagtatctta aagcatccaa gtaatccttt
     8701 tcttcatttt catcaagtgt atttactatc ttatcattac tatagtgact accaaatgaa
     8761 tcacatgtta caagagtttt atcctctttt atatatgtgt atatagtatc tggccaatga
     8821 agcataggta cagagaaaaa ttctaaagtt ttgtccccaa ttgataatgt atctccatct
     8881 gtaactgcaa catattcaaa gtctttgttt acgatttcct taagatattt aattgctgtt
     8941 tctgaagcta ctacttttgc atttttagct aaatccaaaa gcttttcaac gcttcctgca
     9001 tggtctggtt cagtatgact tacaacaata tagtctattt tttcaaaatc tatatttaaa
     9061 tcatttaatc tttcaatata attgtcaaaa tgcttatctt taactgtctc aaacaaaact
     9121 gttttttctg ttccttttaa tacatatgaa ttataagtag ttccataagg tgtatacata
     9181 attatgtcaa atactcttaa atcagggtca agtgaaccta cccaatataa attttctttt
     9241 atttttagtg atttcaatga cataccgtac tcctcctaaa atagttattt agtctcttta
     9301 taccctgctt gttatatttt aaacaaaatt tattcaaaaa agaaattatt ttaaagatag
     9361 tttaaatctt ctaatcttct ttttctacct tcaatacagt tcccttttta gaatctactt
     9421 ttatgtcata ttcattttta tctttatcta ttcttatttt ataaaaagta acatctaaat
     9481 ctttgtctaa actccattct ttaaccattc cattttgttc ttttaatgca atttccatag
     9541 cttgttgagg tgtaattatg tcatttaaat ctaatttttc ttttctagca actccattta
     9601 aatcttctga atctaacttt tctgttttat cttctaatac atcttttgta ttagcatcaa
     9661 ttttcatctt atattcatta ttatcatcta caccttccac agtataaaca aatttattta
     9721 aatctctctc aagacttaaa tctactatat cagcatcctt gtatttatct ttaaatatgt
     9781 ttattgcatc tgtgtatggc actttaaaat cttgttccat taaattttca ttattttttt
     9841 catcttttaa agcattattt gaagcatttg cctccttttt tgtattatct tgtgcttgtc
     9901 ctgaatcctt agttgggctc ccactacatg cagttaataa acctgctata agtatactta
     9961 ccactaataa atacgttttt ttacacataa aaatcctcct taaatatatt aatttattct
    10021 atattatgcc ctctaaaccc tttatttacc cttttatatt aaaaattcct cctattttat
    10081 caattttaaa actttatatt tactaaaatt aaagttacat aaaaattata ctgtctcaaa
    10141 atagaaacaa gcatcttttt cattgttgaa ataataaaaa tatattttat ttcgtacaaa
    10201 aagagtgcct catgaactaa aaagttcatt ttgagacact cccattcttt tatattttta
    10261 cttacaaaat aacagtactt attaattttt tacaaacctt ataagtatat ctatattata
    10321 ttcttaaatt taaacactat attaaaaaat taaattttgt ttaattcttt tactgttaaa
    10381 tccattttag gatatggaat cgtaattcct tcttcgtcaa acctaacttt aacctcttct
    10441 agtaaatcaa gataaattgt ccaataatct tctgtcttgc accaaactct agttgcaaat
    10501 ttaacagcat tatctccatg ttctactact cctataaatg gttctggctc atttattatt
    10561 aatttatgat tattaacaat atctttaagt actcttctaa catgtagtat atcatcttca
    10621 taccctacac taaaagtcaa atcaactcgt ctagtattct ttgctgaata gtttattaga
    10681 ctatcatttg atacagaacc attaggtata agtatctgtt tattatctgg agtaaccaat
    10741 tgtgtataaa aaagtcctat ttgttcaacc gttcctccat ggcctgctgc ttctacgtaa
    10801 tctcctactt taaaaggtct aatcaatagt ataataaatc ctccagcaaa atttgataga
    10861 ctaccttgta aagccaaacc tattgctacc cctgctgatg ctatcacagc agcaaaactg
    10921 gacaaactca ttccccaata ttccagaaca atgattataa cggcaacttt aagtacactc
    10981 aaaagaagag acttcaaaaa ccttcttaaa gttaaatcaa tatctctttt actaagtact
    11041 aacataacat ggtttacaaa tttttttatt attttaaatc caatagaaag tataagtata
    11101 cctattataa gttttactcc gcttgtagta gcccattcta gcagactttc cattatacct
    11161 cccatatcca tcttattaat gtctaaatta tttaaatcta acttgcttat atcatctatt
    11221 ttcgtcgtat ccataaaaaa ctccttacct tatattttag aaccaatatt atagtttaaa
    11281 gttcttattt tctatttaat ataatacaat accatttgta catagtaaac aagatttata
    11341 ttccaattat acatcttagg aaaatgcaaa ctatttgtta tatatatgca aaatataata
    11401 ctaaattaaa ctattaataa aacactatat agccttacaa tcatcatttt ttacccctat
    11461 atgagatgat actatagtta taatacttag tactataaat actacaaaac cttttttata
    11521 tccatcaact atattatatg aatttacttg taaagagata gctataatca aattaataat
    11581 tccacttcct ataaattcaa aagtattaat aacactagtt gccagtccag aataaatttc
    11641 tttattttta tacttggcat cattaaaaac ctgtagatgt gacataacta tacatcccat
    11701 aataaagaat gatattggta aaactataat tggtggtttt acctggtata caactacaat
    11761 tacaatccat ataaacaaat ctatcattgc tccaaatttt attatattaa atttgctaga
    11821 ccttatttta gcaaatacaa aatccataat tattgaacca aatataaatc catatgtaaa
    11881 aaatgatact ataaacgcag aaaaactctt actcacacca taaacgtcca ttatgtatct
    11941 tactccccac aagcttataa atgcacttgt aagaccaaca aaagagaaca ttatcattga
    12001 attataccat gttgatttgt tctttataac agacttaata ccatcaacaa tattaacctt
    12061 ctctgacttt tcatagggtt cagtatctac attgaatcca tactcttttg gtgtatctct
    12121 aacaataata tatataaaac atccaattac aacaccaatt ataccgatta ataaaaatga
    12181 atttctccaa ccaacaagtt cagacaaaaa cacaagtgga aaagtagcaa gaactccacc
    12241 taaatttcct acaaatgaaa atttagctgt tgcagaagca aattctgact tattaaacca
    12301 tctcccttga atttttaaaa tacaaagtaa tattactgat gttcctgccc ctacaattac
    12361 acgagaaata tatgctaatt ctatactttg aatcaaacca aataaaattg accctaaact
    12421 tgccattatt attccaagac tgctaatttt tcttgctcca tatttatcaa tcaaaattcc
    12481 tgcaggtatt tgcataaatg catatgcata taggcaaaag gatgctatat ttgaaatttg
    12541 tatagaatta aaacctaact catttgctaa attgtctgaa acaacagcag ttgacatcct
    12601 taaaaataat actaaaacaa atgaaaatgt aattataccc caaacactcc acttagcctt
    12661 tgtataactt ttcatatctt ctccctcaaa tccatatatt tttaaatttt cttgatataa
    12721 cttatataaa gtgtatagtt attcttacaa atttttacac gcaatatcat tatacaacat
    12781 atgtatatat ttaactagaa ataaaatact attcatatta tatacatgaa ttaactacat
    12841 aaataataag agccatcttt taacattact caaaagatgg ctccatataa aaacatttat
    12901 tctaaattta attttttatc taatataaat tttgtagcga aaaataacgc tattgcaact
    12961 acaagactac caagtattgc aagcataaaa ctaggactct gataaagatg atataccata
    13021 tttgtatctt catttggtaa gatgcttcca actgcatctc caacaattga tattactata
    13081 tttatagcta taaaagaagc aaatgccact atgtttctat gtttattgaa ttttggtaat
    13141 tgtcccattg agattgatgt atataataac aaaataaatg atgaatatga aataaacatt
    13201 actactacta ataataaaat atctaaaaag ttttcagaaa tccatttaaa actagtgtta
    13261 aacaaatcta aaatttcagg tagtaaaatt ccagaagtac caaaaagcat aagaactcca
    13321 aaagtgaata ctgcaactat aaaactaagt acagcgtata taagtgctgt tatacattta
    13381 gataatataa gtgaactagt gctaacagga agagtaaaca ttaaataacc ttcatccccc
    13441 aaaagatttt ttctaaacct ttgaattgta actacaatag ttaaaactcc taatgccata
    13501 aaaagacttg ttaatactag tattcctatt ccctgtacct gaagtatatt tgtattcata
    13561 aatattccat taaatacagc tactatcaat atggctatat ataacggtat aaatattctt
    13621 ccacttgctt ttaactcata ttttaataat ttacctaaca tttaaattcc tccctaaata
    13681 atgcatctat agattttcct ttttcttctc ttattgcttc aacgtctcct tgtaacacta
    13741 tttctccttt tgatatgaat attatctcat cacatatgtt ttctatttca cttattaagt
    13801 gggtagcaat cagtaaagta ctatcttcag aataattttt taatatagtc ttaagtatat
    13861 atgaccttgc agctgggtca acaccaccta taggttcatc caatatatat atgctcgcat
    13921 ttcttgacat tactaaaata agttgaactt tttctttagt tcctttagac attgtcttta
    13981 gcttttcatt tacatcaata tctaagcttt taatcatctc atttgctctt ctaacatcaa
    14041 aatcactata aaaatcatag aaaaacttaa gtaaatctga taccttcatc caatcattta
    14101 aataagttct ttctggtaaa tatgaaacta tttttttagt atctatggat ggtttcattc
    14161 catttatcat aatttcacca ttatcaggct gtaataatcc attcattagc ttaatcattg
    14221 tacttttacc acttccatta ggccctaaca gcccgactat ttttcccttt ggtatattta
    14281 aatcaatatt ttttaaaaca ttcttagttc cataactctt gtttacacct ttaaattcaa
    14341 ctattccatc ttgagaaaca ttgcttatat ttttatccat tttataattc tcctttcaga
    14401 ttttctgtaa taagttttag catttcatct ctatcatatc caagcttttc taaggttact
    14461 tttaatgtat ttatctccac attagctatt tcctctttca tagcttttat tttttcctta
    14521 tcatctgtta caaatcttcc tttagttctc tgactataaa ctaaaccttg tctttcaagt
    14581 tctgttagag ccttttgcat tgtatttgga ttaacttccg catcttctgc tagtgctctc
    14641 actgtttcta atttagatcc tattttaatt tcccctgaaa ttatttttaa ctttaaatgt
    14701 tctactaatt gtatatatat aggtttgtta ttatcaagtt cccattccat gttcatgcct
    14761 ccttattata ttattgtatt aatttattaa tacaataata taataataca aagtatatgt
    14821 caattacttt ttagtaaaag tataaaaaat ttcacaaaaa aaatgaacac tacattagag
    14881 tccattttta acacaattta ttaaattctt tatattacag attaattacc ctcttaccaa
    14941 gttggataca attaaatgtt caaattcttc aattggcatt ggctttccaa acaaatatcc
    15001 ttgtgccata tcacacttta cttcttttaa aagtttaaac tgttcttctg tctcaactcc
    15061 ttctgatatg actttcatac ccaactcttt tgccatttct attactttag taacaattat
    15121 tttgcttctt ttactattat aagtttccat aataaatttt tggtcaagtt ttaagatatc
    15181 cataggaatt tcttttaaca tatttaatga tgagtaccct gaaccaaaat catccataga
    15241 tattaaaaat cctatttttt ttaaattatt cattatatca attaaaatat caaaattatc
    15301 aaatatgata ctctccgtta attcaagttc tatatacttt gcaggtatat tgtatttatc
    15361 aattatattt ttgtatgttt ctataaaatt attgcaatat aaatgtattc ttgaaacatt
    15421 aactgaaaca gtaactggat ttattcctgc atcaatccat tttctaaggt atatacatac
    15481 ttcttcaagc acatataaat ctaaatttat aacaaaaccg tttctttcaa aaaatgggat
    15541 aaattcattt ggaggtatta gccctttatc aggacgtttc cacctaacaa gtgcttctga
    15601 accttgtatc tctcctgtat taagatctat cttaggttgt aagtatataa taaactcttt
    15661 attttctaaa cttgaaaaca ttgtattttc catctctttt tcttttatct cttggtcatg
    15721 aatcttatta tcataaaatg catatgaact tttatgtcct ccctttgcca ttttatgagg
    15781 tatattagcc ctatctatga ttgagttgat atctctatct tctggaagta taaaatagat
    15841 tccacaactt aaaactaact tatacttttc ttcttgatta ttattaaaca agtctaattc
    15901 ctcaaatata gtttttaacc tctttgttat atcttcttct tctatatatt tcataattat
    15961 aataaaatta tccgcagata ttcttgcaaa catttcctct tcaaatatat tactcttaat
    16021 aatttttgtt aaattacgca aaatttcatt cccaacttca tatccaaata aatcatttat
    16081 atatttaaat ttatcaatat ctatatataa taaagcatac tttacttgag tatttttaca
    16141 tagtattttg ttagcatcaa ttacaaattt atctatagta tttgctcctg taagattatc
    16201 tgtatatgct atcttttgca atttaatttt aaaatttaat ttgtcatata ttatatagaa
    16261 tataagcatc atacatatta tagccataat aagtattatt tgtattgcat atttgctaat
    16321 aaactgatta ttagacacaa tagactcaaa gttatttatg gagttgtttt tatagagttt
    16381 gcataaagaa tagactggtg ttgtgtatgt aatttgtaaa attatacaaa taaataaaaa
    16441 aaacatcaat tttttttgta aattcctcat aaaaatttcc tcccattaaa acacctctat
    16501 tttattagca aataagtaca tctcaaaagt tt
//
LOCUS       DAELSY010000086        16383 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.086, whole genome shotgun sequence.
ACCESSION   DAELSY010000086 DAELSY010000000
VERSION     DAELSY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.086"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(258..668)
                     /locus_tag="KPG09_002447"
     CDS             complement(258..668)
                     /locus_tag="KPG09_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967050.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(884..2266)
                     /locus_tag="KPG09_002448"
     CDS             complement(884..2266)
                     /locus_tag="KPG09_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG0967051.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKSGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIEQSNYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(2277..2636)
                     /locus_tag="KPG09_002449"
     CDS             complement(2277..2636)
                     /locus_tag="KPG09_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0967052.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(2841..3620)
                     /locus_tag="KPG09_002450"
     CDS             complement(2841..3620)
                     /locus_tag="KPG09_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBG0967053.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
     gene            complement(4081..4692)
                     /locus_tag="KPG09_002451"
     CDS             complement(4081..4692)
                     /locus_tag="KPG09_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0967054.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
     gene            complement(4784..5773)
                     /locus_tag="KPG09_002452"
     CDS             complement(4784..5773)
                     /locus_tag="KPG09_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBG0967055.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NRESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            complement(5840..6703)
                     /locus_tag="KPG09_002453"
     CDS             complement(5840..6703)
                     /locus_tag="KPG09_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBG0967056.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            complement(6809..7405)
                     /locus_tag="KPG09_002454"
     CDS             complement(6809..7405)
                     /locus_tag="KPG09_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBG0967057.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGLFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            complement(7445..8248)
                     /locus_tag="KPG09_002455"
     CDS             complement(7445..8248)
                     /locus_tag="KPG09_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBG0967058.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKSFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            complement(8284..8724)
                     /locus_tag="KPG09_002456"
     CDS             complement(8284..8724)
                     /locus_tag="KPG09_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBG0967059.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            complement(8737..10839)
                     /locus_tag="KPG09_002457"
     CDS             complement(8737..10839)
                     /locus_tag="KPG09_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBG0967060.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESTDEKEAIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKNVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQINEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMHGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            complement(11096..12799)
                     /locus_tag="KPG09_002458"
     CDS             complement(11096..12799)
                     /locus_tag="KPG09_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBG0967061.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINRV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            complement(12825..13784)
                     /locus_tag="KPG09_002459"
     CDS             complement(12825..13784)
                     /locus_tag="KPG09_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0967062.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNTRTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            complement(13920..14438)
                     /locus_tag="KPG09_002460"
     CDS             complement(13920..14438)
                     /locus_tag="KPG09_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBG0967063.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            complement(14481..14966)
                     /locus_tag="KPG09_002461"
     CDS             complement(14481..14966)
                     /locus_tag="KPG09_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBG0967064.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            complement(14963..15580)
                     /locus_tag="KPG09_002462"
     CDS             complement(14963..15580)
                     /locus_tag="KPG09_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBG0967065.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            complement(15646..16008)
                     /locus_tag="KPG09_002463"
     CDS             complement(15646..16008)
                     /locus_tag="KPG09_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG0967066.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVNNVLK"
BASE COUNT         5258 a         2967 c         1605 g         6553 t
ORIGIN      
        1 tttaacttag actttatttt tttattactt atttgagtct tatttatttt attctcactc
       61 catatgttca attacatttg tctaaattct ttgatacttt tattacataa ttttaacaag
      121 cttaagtgtt atttgggtag tgcagttgat aattatttaa ataatcatca actgcatgtt
      181 tatatcatta cagtagctta atgtatttaa tcatacttac ttaggatttt gaaatatctt
      241 agagattata tttaacatta gtcaattgct gcataattgt aagcaattga aatttgagac
      301 ccatcttcat tttctatagt gataggcttc ataattttta atccttcagt ttttatcttt
      361 tctaattctt gttccatttt cttaattgtt tcctcaaggt ctttttgact tacatctttg
      421 actgcattag ggtctttagc atagtctttt aattcttgaa tatgtttagc atactcttca
      481 taagtataat gctcaacacc atacttttcc aattcagtag ggtcagttat tcctgatttt
      541 attagagatt caagtttagg taaagcaata ttagatgaat catttccatt tgcaaatact
      601 gctccagttc ctcctagtac cattgcagat accatcattg cacttataag ggattttttt
      661 attttcataa ccaatcttcc tttcttagag atttatttgg gtagtgtagt ttaaaatgac
      721 tattgcaata gttatcaacc acatctttag actaccatag tagtcatatt tgtgtcaagt
      781 ctttttaaaa aacttttcaa taaaaaatac tatctcaaaa caaaattaat tattttgaga
      841 tagtatttta attctaaatc ttttatatta ctaagattta tggttactct atccatgttc
      901 tatattgttc tttattaact gctaacaaat tcccttttct tgatatgaca aagcgattat
      961 cattatagtt actttgctca attgaaactt ctcctgaatc agtataaagt acatccttac
     1021 tattactaga tttatttaca tacgtttctt cactattctc agaattagtt actttgctca
     1081 tagatgattc ttcatgctct ctaaattcaa ttacagatac ttttgccgta ctaaaatgag
     1141 tctctccatc acttttagct ttactaacaa gaatatcaga ttctactttg ggagaatata
     1201 aatcagttga tttactactt attgaaaatc cacttttatc actttgttca ttttttgtta
     1261 aaccaaaaga ttcaacctca ttaatctctt tattttctgt tgcaaaaaca agacctgtcc
     1321 cacaaactaa tacagttgaa actaaaaaag taacataagt tgatttttta aatttcataa
     1381 tattaactat tctcttttct atttttgtct ttccaaatcc actacatatt gtcattgaaa
     1441 atggattatt ctcctctgct agacttatta gtgtggttgc ataatcagcc ctatattctt
     1501 caccaagtat actcataacc ttttcatcac aactaaattc tatgtcttta tttagtaaaa
     1561 aaaacatcac ccaaactaag ggattaaacc aatggacaac aagagctaca agcataaata
     1621 actttaacag attatcaaag ctattaatat gaaccatttc atgagttaaa acaaagtcta
     1681 aagtttgatt attagcataa ttcatttgct ttggcaaaat aattcgtggt tttaaaatac
     1741 cagatgtaag tggtgtccta gtaaattcag atatgcaaac aataaatctt ctaaaacggc
     1801 tattcttaca catccatttt ttaatgaact catgattttt tatatgaatt gaagttttaa
     1861 aaagattaat tattttaaaa tatctaaagc ataaaagtat gctaaacata atagtaacta
     1921 taaaagacaa aatgcccaac atttccaaca tcataatatt ttcaatatta gacattattt
     1981 caaattcgtc agaattgttt ttacctgtag agtcactagc tttcttaaga gtataatttt
     2041 ctgttgatgt attacccaat tctacatcaa aagtcgattc aattatacta taaatactaa
     2101 aaccagaatt taaacttact ggtattagaa gtttaaataa aacaatcaac cacaataata
     2161 caaatacctt tttaggtaat tttttaaata aacatgctct tataaaaata actattaaaa
     2221 gtaatactcc agaagtaaaa ctcatttcta acaatttaat gaacataaat cttacctcac
     2281 tttaattttt caacaatttg tttgagtttt gtaatctcat cacttgaaag attttcttta
     2341 ttaataaaag ctgaaagaaa tttttctgaa gaccctccaa acattttatt aattaaagtt
     2401 tctgtttcct gtttttgtac ttcctctttg cttattaaag ctttacaaac aaaatttggc
     2461 tcaattctct ctattgcacc ttttgacact aattttttta taacggtata cgtagtgttt
     2521 ctattccatc ctgtttcttc ttttaaaatt tttactaact ctccagcctt gctttctcct
     2581 ctctcccata gaacttccat tactttaagt tctgaatcaa ataattttat ctccatataa
     2641 aatcgctcct caaatatagt tatcacaata gtcattacta taataaaaaa ctatcatagt
     2701 agtctatact atgtcaatca tttatttttt aataataaaa actgtctttt taaaaaattc
     2761 ttataaaaga cagttttatc aatgttatac tttaatgttt aattaaagcc atattttatt
     2821 taaatgtata actatctatc ttagtacata gtataagatt tttctttatc taatagtata
     2881 tcctctacat ctgggttttc ttttactaat tgtctataaa aattacaatt agtaacacct
     2941 acatatggaa ttagccatag tagacctatt ccaagagtag ctattgaaag tatataccat
     3001 cctataaaac taaagtttag tacaaataat ttaagtacat ttcctttcat catatctaaa
     3061 ctagcaccca ttgatttaaa tacacccata ttatccttgt cttcacaaat taagaaagta
     3121 gcttgagaaa catataatag caatatataa accggtaaca taagaacaag tattataaga
     3181 actagagtta ttccagtaga aataatagtc cctattccaa cattatcact tgataaatat
     3241 tcaattgctc cactagtaag taaaactcca aatgataaaa acgctcctat aattgtgatt
     3301 ataaaagaat aaatataaac aataattgat tcaaataaat aaaaaccaaa acttcttaaa
     3361 taaattctcc cacttacaaa gatatctcct atcttgactt tttcatcttt agtaattttc
     3421 aagaacatac atgtaaatcc tgctgaaacg caaaaagcta ttacagccac tacaatagta
     3481 gtcaatggtt caaaaggtaa tcctacaatt gatattaata ctatcaatgc aatatatact
     3541 gccatagaaa ttgcacatat tccccagttt ccagataact gagctttaga tactcgtttt
     3601 aattcttgcc ttgataccat aaaaaacacc cctttcattt tataaaaaaa cttttaaaaa
     3661 tataaataat cttttaacgt ataatattaa tatatcatat tttcgactat ataagataca
     3721 attattcacc atgttttatc aagatatgcc ataattgtat tactagtttt atatagtcaa
     3781 cattaccaaa tacatcaaat aaatttgatt aattattcaa caagtaataa aataatttct
     3841 attaattgta ctttatttag taaacaaatt atttaataaa aaaattactt aatacagtaa
     3901 attaattggt aagataaatt atttattatt agtaaagtaa attatttact atttatcaag
     3961 ataaattatt tataaaataa atcatttata aaatataatt tatactttga aaataaaata
     4021 aactatcttc caaacatttt attatatgct taaaagatag ttttgtgtct ttaaaataaa
     4081 ttaaattgta tatattcctt cacataactt ttgtatatta ctaggtggtt gccatttacc
     4141 atctttttta gtatatgtaa tcatagttgt aacttcctta gttggtttct tattgtaaag
     4201 gtctataatc atttttgtca tatcatcttg aacttctttt tcttttttat cgtaattctt
     4261 ttcatcagtt acataagatt ttactaaagt gttcgcttca ctcattactt tactagtatc
     4321 taaagcttta cttacaactt tcaaagttat cttatcctta ctagaagatt ccactgttac
     4381 tgtcttttca acaccttgaa ttaatttaac aattgtttcg tatatctttt cagcattttc
     4441 tttaccataa agaggtgtca attgagcaat cattgaaacc ttgtccttgc tgttataagt
     4501 ttgaatctgt ttatattcag cttctgttaa tgcatcagaa attccttttt catcatactt
     4561 atacatttct aatgcagcta aattagcttc aaaattcgac tttacttttt cttctgcatt
     4621 agaacaccct atagttagaa ctaaaactag agccataatc gtagacaata ttaacttttt
     4681 taccattttc atacaaacat cctccctcaa ttaaaataat aaaaaattta tttatcaaca
     4741 ttataacata aatttgtcta actatagaat tattttgaat attttaccac ttccagtcta
     4801 ttccaatacc gttttctttt gctttatcgt atattctttt tgctgttacg acatcttgta
     4861 cacctatccc aacagtttta aatacaatta tctcatcatc atcttctctt ccaggaatag
     4921 ttccatttat aacgtttcct aaatcaccac taaaatcatc tttacttatc ttaccttctt
     4981 ttaatggaat taatatatct cctgactcag ataaaactgc atctgttgat tcaaaaaata
     5041 tcttagacgc tctagttaaa cagtttgggc acaactcttg catatctggc atatatgaac
     5101 ctactgcact tattaaagct ccttttttca ctaagtttcc attaagtact ggttgttttg
     5161 atggagtagc taaaactatc acatctgcgt tatctatggc ttcatcagat gattcaactg
     5221 caactatttt agtattatat ttacttaatt ttaagttcat ttcttctgca aatttttctc
     5281 tactttccct gtttctacta aaaactctta cctcatccaa atttctagct tctatcatgg
     5341 cttcaagttg tggctcacct tgtcctccta gacctatcaa agctccaatc ttagaatctt
     5401 ttttagccaa tacatctata gctgctccag aggctgctcc agtacgaatc tgagttacat
     5461 aagtaccatc caatactgca ctaacttctc cagtttctcc atctaataat agtactgttg
     5521 ctggaattac tggtttgcct ttttgagcat ttttaggaaa tacagataca atttttattc
     5581 ccccacagcc taattcttct acgtatcctg gcataaataa catactagca tcttgacttg
     5641 gaacagatat attggttctc aaaggattta cactctttcc ttcacaataa attctaaaag
     5701 cttccttatc agcttcaatt gcatctctca ttgtaaatac tttttttatg tcgtcttttt
     5761 ttaaaagtag catttttata cccccaattt taataagttt ctatgctaaa tattatagca
     5821 taacatttaa tttaatcttc tatttcaaat caaataaatc tacatactct ggttttagca
     5881 tagaacttat caacgcttta tttattgaaa attctggtat tcccgctgca tatggagtta
     5941 tatcatataa atcaaaatat acaactagat tctcatcttg caagtaaaag ttttgattct
     6001 cttttatacc tttaaaatta tatattccga tattttcttc atcctttttt tcaagttcag
     6061 ctatttgtct tttaatttct tcatctataa cttctttata tttagtatta tccttaaata
     6121 agtccattaa ttctaaaaat tttccagttc tcatatccac attatatgct atatcttgat
     6181 aaaatccatg tgctccacca ctataagtat aatatctcac ttttatacta agagcagaat
     6241 cagtattctt tttaagttca aagtctacat ttgcaacaaa cttatttttc tcgtcaggat
     6301 tatccttaag atactgcttt gcttctttat atgatttatt ataaaattca aatatgtcat
     6361 tttttatctt atcatttgta gacctttcaa caattttatt tgaatttgtt attacaggca
     6421 tgtttatagt actttctaaa tactcatcat ttttcactat tgttttactt cctattgtac
     6481 ttactgataa agtttgttta ctatttgtat taccctttgg taatgactgg ttactcataa
     6541 cagtcactgc ttccttatta ctatttaaat taattgaagc atctttcctt aaattattat
     6601 atttatagtc atgtttaatt gccaaaacag ccattatagt caatgttaaa acaaatacat
     6661 atcttaaaac tttagctttt gtatcgactt taaaaaattt catattatca ttttcctcct
     6721 aaatataaat ctctcttatt atatttggag tttctagggt tttttatcca tataatttaa
     6781 taaaagatta atttttattt ataaaaaatt aatctttttg ttttaaaata ttaatatgtc
     6841 ttatcaaaca gtttgaaatt tcaccaagag gtaattgtaa cataacagaa cctctatcaa
     6901 aagctaccat tggcattcca tatactatac aagaattttc atcctgtcca attgtaaata
     6961 aaccattttc tttcattttt aaaagtccat ttgctccatc ataacccata cctgtaagta
     7021 ttattcctat tgaattttta cctgctactt ctgctactga atcaaacaaa acgtctactg
     7081 atggacagtg accacttatt ctttctcctt tttcacaagt tacataatat ccttgtttat
     7141 ccgtacacaa tttcatctgg taacccccag gtgcaattaa cacatttcct tgttcgactc
     7201 tatcattatt cttagcttct cttacattca taatacactg cttgtcaagc cgttgtgcat
     7261 acatataagt aaatatagct ggcatatgtt gaactacaac aatccctggt gttgattttg
     7321 gtaggtcttt tataatttca agtattgctt ctgtaccacc tacagatgcg cctatagcaa
     7381 taaccatatt attaaaacgt tccacattac cgcctccaaa aaatcatata tcaaaaatac
     7441 ctttttatcg tttatatata gcaggtttta catatttgta tttagtacta cttctgttga
     7501 ttacttctgc atgtcctaca aacaaaaaac tatctttttt ggtagcatta taaaatttat
     7561 ttataagttc ttctcttgtt tttaaatcaa aataaatcat aacatttcta caaaaaatca
     7621 aatcaaaatg ttgatatgag aaatcattca ttaaattaaa tgacttaaaa attacacttt
     7681 ttctaatctt atcacaaatt ttaaatgtac catccttgtt gtctataaaa tacttcttct
     7741 tccataactc aggaacattg ttaacatttg attctatgta tatacctctt ttagcttttt
     7801 caagtgagtt agttgaaata tctgtggcta tgattttaat gttccacaac tctttattat
     7861 atccaaaata atcatccaaa gtcatagcta ttgaatacgc ttcttctcca ctagaacatc
     7921 ctgcactcca tatatttatg tttcttattt tattattttc ttcccaaaaa ggaagtatac
     7981 tattctgtat aaactcaaaa tgttgaggtt ctctcataaa aaatgtgtgg ttagtactca
     8041 atctatttat taggtttata gcttcatttc ctgtcttatc tttaaataat agattcatat
     8101 attgactaaa agaactaagt tttttctcta tcattgtatt gtataagcga ccttcaatta
     8161 acgctctttt tttacttaaa tcaataccat attctttttt tacatggttt acaagaacta
     8221 taaattcttc atctgtgagt tttatcatgc aaaaacttcc tcctgctaat ttatcagaac
     8281 taactatatg ctattaatca aaaactttcg aatatctaaa attaatatag aattatcttc
     8341 aactttcgca atctctttta tatctttatt aatattagtg ttgttagaat tagtcgtctc
     8401 tagtaaattt agattttcta tatgaataac atcaacaact ttatctacaa taattccaac
     8461 ttgatactca tcaacctttg taattattat acaagttctc tcattataat cagctttagg
     8521 aagattgtac tttatatgca tattaataac tggtattatt cttcctctca aattaataac
     8581 tccacttata tatgttggaa gacatggtac atacgttatt gattgaaatc ttatggtttc
     8641 aatcacattt tctaattcta ttccataaat aacatcattt acataaaatg ttaatacttg
     8701 tttaataatt tcatctttag ccatacttta aatcccctaa tatatttcta taatgctatc
     8761 tgcatctata attaaactta tacttccatc cccaagtatt gtacatcccg ataaaccctt
     8821 attttttata ttaaatcggt tgaaataaac tggaaatggt ttcacaacaa cttgctgatt
     8881 acctattatt tcatcaacaa aaatgcatac tgctttatgc tcattttcaa taattatcat
     8941 attaccatgc atcagattgg ttacttttgt ttgaatatta tatattttat gtaaccttat
     9001 aatgggaata catgctccat atattgtaat taactctgtc ttatttcctt ctgtaattat
     9061 ttgatttaag tctgtaagtt taaatgttct tcttatggat gatattggta aagtaaatag
     9121 tgaatctcca actttaaact tcataccctc tactatagaa agagttaatg gtatttttat
     9181 agtaaaggtt gtcccttcat cctgtttact ttttacttca attgaacctc caactttttc
     9241 aatactttta cgaacaatgt ccatgcctac tcctcttcca gaaaattcag ttatagattc
     9301 ttttgtagaa aaacctggag tcataatcaa agaattgacc tcgtcatcag tatactcatt
     9361 aatttgatgt ttaagaagac cttgttcctt agcttttgcc aaaatcttat ctctcataat
     9421 gcctctacca tcatctgaaa tacttatcac agcttctccg cctagattct cagcagataa
     9481 aactatcttt cccacttctt ctttaccttt cgctatacgc tcctcaatag gctctattgc
     9541 atggtccata gcatttctta taaggtgcat aaaaggttct gctaaattat caattacatt
     9601 cttatctacc tctgtctctg ctcctatagt tattaactct acatttttat ctaatttttt
     9661 attcatatct cgtacaattc tatgcatttt ttgaaataca ccagcaattt gaatcatacg
     9721 catagacatt actatatctt gcaattcatc tgtaagcttg tgtaattgtc ttgttgcctt
     9781 attgaaatta tctatatcaa cacctgtaga atttacgcta tttacaacca ttgattccat
     9841 aattacaatt tctccaacta tattttgaag taaatccaat ttttctaaat ttacactcat
     9901 aatactctgc ttacttattg gtaaatgttc attttcttca cttagactat ctttttccat
     9961 atcagttttt tcatttaaaa catcttcttg tatctcatct tctgtatagt tattttgata
    10021 acttttactt actttattat tatcttttaa tttattttct actgcatcat tatttgatgt
    10081 atttttcatt tctatttctt ttttactaat atttatgtat tcttttacat agagattatc
    10141 ttctatagtt ttataaacta ttgccttatc ctctttagct acaaattgta tataaaaccc
    10201 ataactttta atatattctg tagactctgc atttgtttct acatcttttg gataaaaatc
    10261 tataaattca cagtaatctc taagttcaaa tacaagcatg aacgctctca cattttccat
    10321 catgcaacct tgttcaaaga ttatttttac tacatcagag tcttctttgt attcaaaatt
    10381 tgaatcgatt gcttcttttt cgtctgtact ttctaaattt gcattatcct ttgatttcat
    10441 agaacttgat aattccaaag tactcacata ctcttcaatt ccttcaataa aattagagta
    10501 atcagaagat atacaacctc ttgattgtag ctcatctata tctcttctta taaaatctaa
    10561 tgattgaaag actaaatcaa ataataggtc attgtctaaa acttctattt taccttcacg
    10621 tatagcataa aacaagtctt ctacactatg tgctaaagca gatatattgt ctaaccccat
    10681 catagatgct gaacctttta tcgtatgcat tattctaaaa gtttcattaa tgttgttatg
    10741 agtaaatact ttctcttttt ctatttgaat cataatttca tctaattgtt cgagtaataa
    10801 acttgtctca aatataaaca tatctaacat ttgtctcata ctcggttcaa tataattcac
    10861 atttatcacc tacgacctac aaataatttt ctattatata tgcatctcat aatttaaatc
    10921 cttgatgatt ctcttatctc actatatgta cctcataatt taaatacaca atcattctct
    10981 tatttcaata tatatacatc tcataattta aatacatgat aattctctta cttcaatatt
    11041 cacctcataa tttaaataca tgataattct cttacttcaa tattcacctc ataatttaaa
    11101 cccatgatga ttctcttact tcactttcct ttagatgaaa tttttctact aattctttta
    11161 aaatttgagc ttgagcagac atctcttctg aggctgctgc actttcctct gctgttgctg
    11221 agttcatttg gactactatg gaaatttgct ctattccaga taaaatctgt gtaattgcta
    11281 cttcttgact atgtacagcc gaagaaatac cattaatata attagtagtt ttttctacac
    11341 catttactac caagtccaat gcatttgcag tctctttagc aattctgaca ccattttcta
    11401 ctgttttaat agcttcttct atgagaatag ctgtattttg agctgctttt gcacttttac
    11461 cagccaaaga cctaacttca tctgctacaa ctgcaaatcc tctaccatat tgacctgctc
    11521 ttgctgcttc aacagctgca tttaatgcga gaatattagt ttggaatgct atctcatcta
    11581 ttgttttgat tatttttgat atttcactcg atttttcact tatttcaccc attgatgtca
    11641 tcatatcatc catacatcta cttccaaaac caacttgtga tgcagattgg tcagatacct
    11701 cacatgcaag actagcacta tctgcacact tcttaattcc atctgatatc tcattaatcg
    11761 tggaagctaa ttcttctact gaactagctt gttcagtagc accctgtgaa agagcttgtg
    11821 cacctgctgc aacttgttct gaagcacttg caacttgttc tgctgataaa tttatttcag
    11881 aaaaatcacc attaaaagag tttattatac tttctaaaga tattttaatt gatttaaaat
    11941 cacctataaa ttcttggtct atctctactc ttaaatctcc ttttgcaata ctagttaatg
    12001 tactgtctat aaggtctatg taatttaaaa gcatactttg catctttttg acagaactca
    12061 ctaatagtcc tatttcagat ttactttctt caacgtctat acttgttctt aagtccccag
    12121 atgcaattct tgaaaagtgt ttttctactc tattaatagg aatcactatc tttaatttta
    12181 ttactgtata ttctattatt gtcaatatta ttaaaaggga aagtataaat attgttctta
    12241 atattagact ttgtaattct ttttttactt ctacaatttt ggaatttgaa cgggtaacta
    12301 tatcctcttg aaattcattt attaaagaat ccattttctc ttgggagttt atatagtcac
    12361 taccaaaaat tatactttga gctttactct tatcattact ttcgactgcc tttattgcac
    12421 tttgttctgt tggtactaaa gaatctgata tttcttttgc ttctaagatt ttatttgact
    12481 cgctctttgt aagaccaatt ttttctaatt tgtttaaagc tgattctctt gtttttccgt
    12541 ataattcttt ttcataaata tctttattct ttttttctcc tgtaaataca tatcttctta
    12601 atacatcact tatctctcta gttgtatctt ttatatactg actttgagtc ctcaaattat
    12661 tcatatcatt aatacttttt tctacttttt caaatgcttt ataagatgaa atagaagaaa
    12721 aaatccctgc tagaacagcc aatactataa taatcagcaa tacaattgct aatttagaaa
    12781 gctttacttc tcttttcata tctgtactcc ttcataaagt aagtttatat cttttttaat
    12841 cctttcattg tactcactac gctacaatct tttcctaagt ttttaccctc atacattgct
    12901 ttatctgcta aggatatgtt ttcttctaat gatatatttg agtttacttc tactactcct
    12961 aatgtaatag tgcactttat tttatgttct ttatagttga aatcaaactc ctttatctct
    13021 ttacggattc tttctgaaag tatcttagca ccttctattg gagtatttgt gagcattatc
    13081 aaaaactctt ctccacccca tcttgataca gtatcaattt gtctacaatt cttttttatt
    13141 atacttgata ttactgttaa cacgaaatcc cctgcttcat gcccataagt atcattgata
    13201 accttaaagt catcaatatc accaaggata agggatattg atgtttcact atatttatta
    13261 attcttcttt gctccataag ctgattaatt atatgtcgcc tattgtaaag acctgtaaga
    13321 taatctctta caacgagtct attcaatttt tcaagagaag attccagttc attatttatc
    13381 ttttttaatt tatcttgaga tttctttact tcaagatgag ctataacacg agcttttaat
    13441 tctgtcatac taaaaggttt caccatatag tcaattgctc ctacaccaaa tccttttact
    13501 atgtctgtat ctgtattttt tgaagttata aaaattatag gtatatctgc tgtacgagta
    13561 ttagatttta cttccttaca aaactcaaaa ccagagttat ctggtaaaat tatatcaagc
    13621 aaaataatgt ctggtataaa ttcttcaata aattcttttg cctctattgc attaaatgct
    13681 tttttaacct taaaattatc ttttgtaagt gttttatcta ttaagtcaca catcagaaca
    13741 ctatcatcaa cgactaaaat tgttccctgt tgcttatttt tcatatacaa acctctctta
    13801 aacataatat attcaaattt taatgattta aaatttttat acttaataat tataccacta
    13861 tattcttaaa tgttctcatc acaatctttt tataataaca ctccaactac atccctatgt
    13921 tacatgatag tgcaatcaaa cccattgatt aattcatcta ttaaaacttc atctttatct
    13981 gtcaaaagtt tctctaaatc tagtaaagta attattttac cttctaattc tccaacgcca
    14041 cttacaaaat aatccgaata ttctatagta actcttggtg gaggtgatat tttacttttt
    14101 tctaagctta ctatatcatc aactctatca accacaaatc caactaccaa gtcctttatt
    14161 tcagttacta ctatatacat gcaatttgta gtcattaatt catctttttt aagtctcaaa
    14221 cgcatatcta ttattggaat gatttttcct cttagattta ttatgccttt agaatatgta
    14281 ggtagttctg gaataaatat aatttcttga tttccagtaa tttctattac cttttcaagt
    14341 tcaaccccaa aatcttgctc atctacttta aactttagat acttaattcc ttcatcaatt
    14401 agtccataat ctaaatatgg gtcatctgga aatctcattt aatacctccc tcctaaaata
    14461 cactttctat gaaatatata ttataatttc ttcttacctt tatttattga atctacaagt
    14521 aattcacccg tctcagtgta aaaacgtatt gttctaccat aattcatacc tgtgtcctca
    14581 gctaatattg gaatatttaa tcctaaaaga gtttttttta ctgcctcaac attcctatgt
    14641 cctatacttg caattactga attgctgctt cctaaaaaca tctgagcacc tccagcaatt
    14701 tttgctttta tatatattct atttgcacct atattctcca tagatttaat gagctcattt
    14761 attcctgtgt cagcaaattt acacttattt tcaatgttaa tagcatcttt actatatggc
    14821 aacataacgt gtaacattcc accaattcca gctactttat ctatcaaaca tactcctaca
    14881 caagagccaa gtgcataagt aactaaaaca tttggtctat aggctatctt catatctgca
    14941 atgttcacta ctatctctct attcatactt ctaatcccaa cctcacgagt atttctctaa
    15001 gtgagctcat ttctggaatc atcaaaatgt ggctagtaaa ataattatca ctcattttaa
    15061 atttattttc tataaataag actttgtcac caacatctga aaatcttatc ataggcacat
    15121 ttagaacagc tccaaccata tcaatacata tatctggaat tgaaacactt atagtcatgt
    15181 ttaacataga tgcaatagca tttacatatg aacttgccat aatatttcct atttccttaa
    15241 ttgctgaaat ttcaattgcc tcaatctcat ctaaaaaagc ttcttcttta ttaaaaagag
    15301 aacttagcat aagttgagtt attgattcat ccaacaaaaa cataaacatt ccattgatat
    15361 cacctttcat atcgagtaaa atacatacta ctttgttttc tggaccacca agcaaatttg
    15421 tcgcttcatt atattcaagt atcttgacca taggtattaa tacttctaca ttgctattta
    15481 tcatggatgc aagtgcagta attgcattcc cagagcctat attaccaatt tctctaagag
    15541 catctatatg aacatcatca agttctaaat aatttataat ataaacccct cccttttatt
    15601 attgcattta tttgcttttc attgtatcag tgtatattta cacttttatt ttaaaacatt
    15661 atttacaact ttaactaatg tttctgtttt gaagggcttc actataaaat ctaacgctcc
    15721 aagttttatg gcttctacaa tcatattctc ttgtcctatt gcagagcaca ttacaacctt
    15781 agcacttgaa tccaactttt ttatttctct tagcacctct aaaccatctt tttttggcat
    15841 tgtaatatca agcaacatca aatcaggctt ttcatattta aatatattac acgcttcttc
    15901 tccatcattt gcttctatta aattattata gccactttta ctcagagctt ctttaattat
    15961 cattctcata aaaatagcat catctactat caaaatttta ttttccataa aactaactcc
    16021 tccatatgta caacaataaa cgcttttatc ttcagttatt gtctatacta tctgactatt
    16081 tttcgctaca caataaatac atttcattga taaataaaag taattattta tgccatatta
    16141 tacttaattc tcttaacata atttgtcaac aaaaaaactt atatatctat tgtaatatat
    16201 gtttggcgtt ttgacgagtt attaaaaata taattgtata aaacataaaa agttatcaaa
    16261 agtaatcaaa ttataataaa acaactttat acctttttta tgtttgtata aaaaattttt
    16321 tcagattcat taaaatcatt tctgtagatt catataggat ttgactttct caatatctaa
    16381 tag
//
LOCUS       DAELSY010000087        16088 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.087, whole genome shotgun sequence.
ACCESSION   DAELSY010000087 DAELSY010000000
VERSION     DAELSY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16088
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.087"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(384..1787)
                     /locus_tag="KPG09_002464"
     CDS             complement(384..1787)
                     /locus_tag="KPG09_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0967067.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            2664..3173
                     /locus_tag="KPG09_002465"
     CDS             2664..3173
                     /locus_tag="KPG09_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBG0967068.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            3201..3395
                     /gene="rpmI"
                     /locus_tag="KPG09_002466"
     CDS             3201..3395
                     /gene="rpmI"
                     /locus_tag="KPG09_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBG0967069.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            3451..3807
                     /gene="rplT"
                     /locus_tag="KPG09_002467"
     CDS             3451..3807
                     /gene="rplT"
                     /locus_tag="KPG09_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBG0967070.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGIEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            4117..4662
                     /locus_tag="KPG09_002468"
     CDS             4117..4662
                     /locus_tag="KPG09_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967071.1"
                     /translation="MNEKLMDCFVNPIKCKLLLEVFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKEGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            4649..4822
                     /locus_tag="KPG09_002469"
     CDS             4649..4822
                     /locus_tag="KPG09_002469"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967072.1"
                     /translation="MKGNKSDLHEVEPIYTENRFMIYICIQSKVSLHGLYFIVPFHPM
                     TWSGTMKFFNFSY"
     gene            5098..7023
                     /locus_tag="KPG09_002470"
     CDS             5098..7023
                     /locus_tag="KPG09_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0967073.1"
                     /translation="MKIKFVIMPLVIFLIVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFISNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            7424..9328
                     /locus_tag="KPG09_002471"
     CDS             7424..9328
                     /locus_tag="KPG09_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0967074.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFKQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEGKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
     gene            9716..10495
                     /locus_tag="KPG09_002472"
     CDS             9716..10495
                     /locus_tag="KPG09_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG0967075.1"
                     /translation="MNYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKVFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVVLGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
     gene            complement(10805..11626)
                     /locus_tag="KPG09_002473"
     CDS             complement(10805..11626)
                     /locus_tag="KPG09_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HBG0967076.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDITVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADVLIISLVSDDGILNDGTKLEDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(12351..13169)
                     /locus_tag="KPG09_002474"
     CDS             complement(12351..13169)
                     /locus_tag="KPG09_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0967077.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNNTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDSVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            13337..13918
                     /locus_tag="KPG09_002475"
     CDS             13337..13918
                     /locus_tag="KPG09_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0967078.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            13915..14109
                     /locus_tag="KPG09_002476"
     CDS             13915..14109
                     /locus_tag="KPG09_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967079.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(14273..16015)
                     /locus_tag="KPG09_002477"
     CDS             complement(14273..16015)
                     /locus_tag="KPG09_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBG0967080.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEDLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
BASE COUNT         6185 a         1898 c         2529 g         5476 t
ORIGIN      
        1 aagtgataga tacctaatta agttgtagat atctgataag aaatttttaa gttttcaatc
       61 gttctaaatt gtgagtataa aaatctacta aatttagtta tgtaaaatat aaattataag
      121 tgtagaaact aggctactag cttttatacc acttcaaaca ttactggaag tagttaagtt
      181 tacagcagtt tagttttatg tagatataat attctgtaaa tagacaaaat taaaatatgt
      241 aattaaaata tgtaagtgtc tcaaaatgaa atttttaatt tatgaggcac ttttttgtat
      301 aaaatcaaat atatttttat aactttaaca ataaaaagag gcttatctct attttgagac
      361 agcctcaaat ttttaaaact atattagttt acagcttctt ttcctatcaa atcttttaac
      421 tcttcatgag tcatagtttc tttttcaaat aagaatttag aaacagaatg taaatcttca
      481 agattatcct ttaacatctc taaagtctct ttataacaaa tttcaactat acgatttgct
      541 tccttttgaa ccttatcaca taagaaagtc ttatcatttc catcaatagt cataaatcct
      601 aagttactca taccatattc acaaaccatt tggttagcta tttcagtgac tttctttaag
      661 tcgtcctttg caccagtaga tttatgatta aatattaatt cttctgaagc ctttccagca
      721 agtaatattt ttatttttcc aatcaactca tccttagtat aaatatatct atcttcatca
      781 ggtaattgaa gaacataacc taatgcttgt cctctaggaa ctatagatat tttttgtata
      841 ggacaaattc ctactatatc acttacaaga gcatgtccag cttcatgata tgctactatt
      901 tttttctctt tttcaaccaa tgcagagttt ttagattcta gccctgcaat aactctttca
      961 agtgccttgt caaaatgttc tgatgtaata cactcactgt catctctaac tgcaaatata
     1021 gctgcttcat ttgctatatt tgaaagatga gccccattga aaccatgagt ttttttagct
     1081 aaaagttcta gatttacaga tttgtctata ggtttgtcat ctgtatgtac ctttagaatt
     1141 tcaaatctag tgtgatagtt tggtgcacct atatgaatgt gtctgtcaaa tcttcctggt
     1201 ctaagtaaag cttcatctaa taagtcaagt ctattggtag cacctattat taatacattg
     1261 gaatctttat taaatccatc catttcaact aaaagttggt ttagtgtttg gtctttttcg
     1321 ttgttacttt caagatgtct ctttgctcct acagcatcta tttcgtctat aaatattata
     1381 cttggagctt cttttctagc tttttcaaat agagttctaa ctctttttgc tcccacacct
     1441 acatattttt caacgaactc tgaaccagta acattgaaaa atgatgagtt tgtttcacca
     1501 gctacagcag atgcaagtag ggttttacct gttcctggag gaccataaaa taatactcct
     1561 ttaggtattt ttgcacccat tttttgatat ttttgtggtg acttcataaa atcgactatt
     1621 tcaaatagtt cttcttttac ttcgtctaat ccagcaacgt ctctaaatgt agtctttggt
     1681 tttgtagaaa gtgaatcatc tgtttgatta tctctttcca tttttgtctg tgcaaagaca
     1741 ggagctggat tgttgatttt cttaaaaaag ttgtttagca ttttcatatt cacacctcag
     1801 gtaattaatt attgttaatc aattttatat tttccatatt tactattttt tattatttat
     1861 ttttcatgga aaaaatatac atgttatcta aataaaaata aatttgtaag atgtatgaaa
     1921 aaatgaattt atgtatatag agatttctac ttatgtattg aataaagtat tacaaattat
     1981 aaattatgta ttgattatat attgagtaaa gtattataaa gcatgtattg attaagtatt
     2041 tattaagtat tgaataaaat attacaaatt tagttgaaat gaaaatgata aaaaatgttg
     2101 tgctatattt tacaaatcat ataaatagag aaattgttgt agcacaaagt aataggtagt
     2161 atattagaat ataatatagg atttgttgtg tagtatagta gcatattaga atataatata
     2221 ggatttgtta tgtagtatag tagcatatta gaatataata taggatttgt tatgtatata
     2281 gtagcatatt agaatataat gtaggatttg ttatgtagaa tatatagagt catgtagaat
     2341 ataaatatat aaaaggattt tgggtataaa tttaaaaatt aatagcaaaa tgagtagtat
     2401 agacatataa ttagtagtag gaagtaatta aatttttcct aaacaaaaaa aatagttgca
     2461 aataatttga ttaagtgata tagtaaataa ggtaagaata aaacaaagaa gaagtccgct
     2521 tctcacctta cgccggaggt tagtaaggta aatataagtt aaagcttaat tttaatttaa
     2581 gtttgtttaa atttggtaac ttatattgcg gatgattacg tcatccgctt tttattttat
     2641 aaaaattttg gaggtgtcct acaattagca aagaattagc aatcaatgaa caaataaaag
     2701 ataaggaaat aagagtcctt tcaccaacag gtgaacaact tggagtaatg cctactaaag
     2761 aagctcaagc aatggctaat agcaaaaact tagatttggt tcaaatctcg ccgaatgcga
     2821 atccaccagt atgtaagata atggattatg gaaagtttag atatgagcaa gcgagaaaag
     2881 agaaagaagc taagaaaaaa caaaaaacta tagttgtaaa agaagtaaga cttagaccag
     2941 gtattgaaca aaatgattta aatacgaaag ctaataatgc tataaagttc cttaaaaaag
     3001 gggataaagt caaagttgaa cttagattta gaggaagaga attaggacat aaagacatag
     3061 gtaaagaagt tatgcttaag tttttagata taataaaaga gtttggagaa ccaacaaaag
     3121 cccctgcatt tgagggtaat aatatggttg taattataga tccaaaaaaa tagatgatta
     3181 actgagagga ggaatttgtc atgcctaaaa tgaaaactca tagaggtgca gcaaagagat
     3241 taaagaaaac aggaactgga aagttaaaga gagctaaagc ttttaaaaaa catatattaa
     3301 ctaaaaaatc tgcaaaaact aaaatgaact taagaaaatc aactttagta agtgatggag
     3361 atgccaagag aatagcacaa ttattaccat actaagatac agattgtaaa taataactat
     3421 agaagaggga tataaaagga ggtcataacg atggcaagag ttaaaaaagc tatgaacgct
     3481 cgtaaaaagc ataagaaaat attaaaactt gcaaaaggat tcagaggatc tagaagcaaa
     3541 ttatatagac cagcaaatac attcgtaatg aaagcattaa aaaatgctta tataggaaga
     3601 aagttaaaga agagagactt cagaaaactt tggatacaaa gaataaatgc agctgctaga
     3661 atgaatggaa tatcttattc aagattaatg aatggattaa aattatctgg tattgaagtt
     3721 aacagaaaaa tgttatctga aatggctata caagacccag aaggatttgc aaaattagca
     3781 gaagttgcta aagcaaaatt agcttagtat tgaatcatga gatgtaaagt ctttgagtta
     3841 tataaataat tcaaggactt ttttattttt tgaaaaatac ttattagtgt catgtacaac
     3901 caattttata atgataaata aaataagcaa tacaatagaa ttttatgaga tttattataa
     3961 gaattgttac aattatttat catagctata aaaagtcata gtaaaaatga tattgatgta
     4021 tgtttatata tgtataaagc tttcaatcaa tcttgaatta atattatcaa catgataata
     4081 ttatcttatt aataatataa ataaggggga gataatatga atgagaaact aatggattgt
     4141 tttgtaaacc caattaaatg caagttattg cttgaagttt tttcaaataa tcgaactaca
     4201 gctaaacaat taggagaaaa atttcaagat atatcaaagg caacacttta tcgttacctt
     4261 aataaaatgt tgaaagatga tattataaaa gtggttgaag aaaataaaat aaggggaact
     4321 gttgaaaaag tatatgctat gaagttagat atagctgatg atgtgaacaa aattatagat
     4381 gagaactctg gagaagaata tgctcaactg tttactcaat atatgatggg atttttaaga
     4441 gaattttatg aatacgctga aagaaaggaa aaggatatcc taaaagaggg ttcaggattt
     4501 tctgtctgtc cagtatatgc tagtattgat gaaatagttg aagtgagtaa aaaaattgct
     4561 agtatactag aacctctaag aaataactcg cacgaagaag gcagaagaat gcattctata
     4621 ggagtgataa tcactccacc taaagaggat gaagggaaat aaatctgatt tacatgaggt
     4681 agagccaatt tacacggaaa atcgttttat gatttacata tgtatccaga gcaaagtatc
     4741 tctgcatgga ttgtacttca ttgtaccatt tcatccaatg acttggagtg gaacaatgaa
     4801 gttttttaat ttttcatatt aatgcctaaa gaaatgaact ttagaccaca tatgcaatta
     4861 ggctacatta tacaattaga ccagatatac agaataaaaa ttagtatttt tatagcagat
     4921 aaattgactg ttattcttct taaaaatcat ctattagtta ttgttagcat attgaaagaa
     4981 attatagtaa tccattgaat aaatgaatat tgcccataat catataaact gctatagttt
     5041 aaaataaagc aggacgttaa attttatagt aatcttaaaa tttaagaggg ggattttatg
     5101 aaaataaaat ttgttattat gccattggtg atatttttaa tagtattatt tgtggggtgt
     5161 caaagtacaa aagatacaga agttaaagca aaagatacag aaattaaaat attaggtaca
     5221 ggtgatttgc attcaatttt gacagactct atggtttcat atgtaaatga ggaaagggaa
     5281 aagaataaaa atctgcttat ggtagatgcg ggagatttct atggtacaca aagtagagaa
     5341 atgtgggaat ggtcttctgg aaaaaagtta attaatatta aaagaaatgg tagagcagag
     5401 tatgatgaca taattaaaag tagtaaagat gaagtaccta tagttaaaga tatggcaaag
     5461 ttgaaatatg atgctgtagt tttgggagat aatgaattta tttcaaatga taagcaaagt
     5521 cttgataaac tagtttctga ttttaaaaat aataatatgc cattggtatc agcaaatata
     5581 tatgaacaat ctggagaaaa ttatgtacaa ccatatgtaa tgaaaaatat taagacagat
     5641 gagggcaatg taaaagttgg aatactaggt cttactataa aagaagtagg ggaaaccctt
     5701 ggtcttgagg attttgaaaa ggataaaaaa gctagagagc ttggagaaca agctgattac
     5761 aaaggaaaat tatatgcaaa tgatttagta gaagatgcaa agaaatggat aaaagttatg
     5821 gaaaaagaaa gtccagacat aatagttgca gttgtacact caggggaaga acctaaaaca
     5881 gatagaaatc caggaaatag aataaaagag ttagcaacta cagtggatgg aatagatgca
     5941 attgtagctg gtcatactca tgaaaaaata gatgaacaca aatataaaaa taaatctgga
     6001 gatgaagtaa ttgttacaca accaggttgg catggagata gagtatcaga gattaatttc
     6061 aaattaaata aagagaatgg aaagtgggac ataaaggaaa agtctagtaa attaaaaata
     6121 attgataaag aagtaagtat agttgctact gcagatttac attcacattt ctctgataaa
     6181 ttaacagttg accttgttaa tgaacgtagt aacccaattg aacctgttgt agtagatgca
     6241 ggcgacttct tagaccctca aacagatgaa atgactcaat ggtataaaga gtggaaaagt
     6301 ataagagata ataatagtga taaaacgatt agtagatgcc caatggtcat ggatatgaat
     6361 cagggaatgt atgatgcagt agtacttgga aatcatgaat ttgtatctga aaaagatgaa
     6421 ttttggtcag atgaacgcct tttagatgct gttatagagg attttgaaca ggtggcaatt
     6481 tctgtgttat cagcaaatat atataggcaa tctggtaaaa attatgttaa accatatatt
     6541 ataaaagatg tggagacgag tgaaggtaat gtaaaagtag gaatattggg acttactata
     6601 aaagaagtag gtaaaggtct taaaaatgta aacttacaag agcaatttca atataaggat
     6661 aaattgtatg caaatgattt agtggaagat gcaaagaaat gggtaaaagt tatgaaaaag
     6721 aaaaaaccag atataatagt tgcagttgta cattcaggag aagaaccaaa aaatccaaaa
     6781 cacccaggaa atagaataaa agagctagca actacagtag atggtattga tgcaattgtt
     6841 gctagtcata ctcatgaaaa aatagatgag catgaatata aaaataagtc tggagaaaaa
     6901 gttatagtga cacaaccagg tgagcatggt aaatattatt ctaaaattac ctttaaagta
     6961 aataaagaaa agggttcgtg gattattaat gataaattca gtaaaacaat tgaggttgaa
     7021 taactttata aaatttatag ataaagtaaa ttatctgata ttgacatgat atataagata
     7081 aagtttctaa tattagtata atgcataatg tgaaatattt taataataag agaataaaca
     7141 agacaaataa aaatatttta acatgtaaga tatagtatga actattatac taatgcttac
     7201 tttataattt gaacatgtta gaataagtct tatttgaaaa ttttagtgta ttagagtaag
     7261 tattatttta ccagtaaaaa atattgagaa ctatatgtag atttgcttaa aaaggaattt
     7321 acatatagtt cttttcgtat aataaatagt acaatcttat atggtaaaga taaagtgtga
     7381 tattaaattt tattatcatc caaaatctaa ggaggagtct cttatgaaaa taaaatttat
     7441 tatagtagca tcaataatat ttttaatagt attacttgtg gggtgtcaaa gtactaaaga
     7501 cacagaaatc aatgtactcg ccacaagtga tttacattca attattccag ataatttaat
     7561 ttcctatgta gaagaggaga gaaaatatga tgagaatcta cttatggtag atgcaggaga
     7621 tttttttgac atgcaaagtg gtgagatgaa taagtggttt actggtcaaa aattagtaag
     7681 atttaaagat gggataccag agtacaaaaa aatagctgaa ccacgtgaag gagaagttcc
     7741 attggctaaa aaaatggcaa agctgaagta tgatgcagtt acatttggga atcatgaatt
     7801 tgtatctaat gacaagcaga gccttgataa attggtttct gattttaaaa ataacaatat
     7861 accattagta tcagcaaacg tatatgaaca atctggagaa aactacgtaa agccatatgt
     7921 aatgaaaaat ataaatactg acaagggaac tgtaaaggtt ggtatacttg gtctgactat
     7981 aaaagaagta ggggaacgtt caaggtttaa aagttttaaa caagataaaa aggctagaga
     8041 acttggggaa caacctcaat acaaagggaa attatatgca aatgatttag tagaagatgc
     8101 aaataagtgg gtaaaggtta tggaaaaaaa agaaaagcca gatataatag ttgccattgt
     8161 acattcaggt gaagagccaa aaaatcctaa aaatccagga aatagaataa aagaactagc
     8221 aacaactgta gagggaatag atgctattgt agcaggtcat acacatgaaa aaatagaaca
     8281 gcatacatat aaaaataatt ctggtgaaga agtaatagta acacagcctg gagctcatgg
     8341 taatagtata tcaaagatta attttaagtt ggataagaag aatgataagt ggtttataaa
     8401 agacaaatat agtgaattaa aggtttttgg aaaggaagta aatataatta caacttctgg
     8461 tctgaatcca aaattttcag atgaaatggc agttaatttg tttaatgaaa gagatagaga
     8521 tgttcaacct atctttatag atactggaga ttttttaaat actaatacaa aagaaatgac
     8581 taagtgggct aaagagtggc aatacaatga aaataaaggc atctataaaa aaataaatga
     8641 atggccaatt gtatatcttg gttatgatgc tgtagtactt ggtagccatg aatttacaga
     8701 ggataaagag acttttgagg gaaaaaaatc tacattggat tatattatag aaaactttga
     8761 agaaatgaag ataccagtat tatcagctaa tatatatgat gaatctggag aaaattatgt
     8821 aaaaccttat ataattcata atgtagaaac tagggaagga aatataaagg ttggagtatt
     8881 aggtcttact actactgata aggatgaaga agacttaagt agctcaagct taagcaaaga
     8941 ctcaaagact tcgaaaattc ctgaagaaac aggaaagtta tatactaata atctagtaaa
     9001 agatgcaagc gaatgggtaa aagttatgca aaaggaaagt cctgatgtga ttgttgcagt
     9061 tgtacattca gataataata gtactctaaa aaatccaagt agtgaagtca aaaaacttgc
     9121 aatgtcagta gatggaatag atgctattgt tgtaggtcat acgaaaaata aggtagaaga
     9181 acatatttac aaaaataagt ctggagatga ggttatagta acacaatctg gagaagattg
     9241 ttattcaaaa ataagtcttg aactaagaaa agaaaatggc aagtggaata tcataaacaa
     9301 atatagtaaa gcaataaaaa tgaagtgaat ttattgatat ataaaccatt attaacactg
     9361 atttttataa tataataaat actgattcta caatataatt aacactactt ttttaaatat
     9421 aatcaatact gattctacaa tattattaat actacttttt ataatataat caatactact
     9481 ttttacaata taatcaatac tgattctata atataattaa cactactttt taaatataat
     9541 caatactgat tctataatat taacactatt ttttatgcta tataacaatt attgaaaatg
     9601 atataaatat aatatctcaa atgatatgaa tataacatct gaatagaggg tgttttattt
     9661 gtaaaaaata agtttacagt ttgagcgtat aaacttatgg taaaggagaa taattatgaa
     9721 ttataagaag attgcaatta taacgggtgc taatagtggt tttggtaaag agtttctaaa
     9781 gttattaata aatgaagaag aaataacaga aatatgggca gttgcaagaa acaaggcgag
     9841 attaaatcaa ttagtagatg aatttggaag taaggtaaaa gttttttcaa aagatttatc
     9901 aaaaatagaa gaagtaaaag aaattggtac atttttaagt aaagaaaatg tatgtataaa
     9961 ataccttata aacaatgctg gttttgcaaa attctgttca tacaatgatt taagtataga
    10021 tgaatctatt aatatgattg atttaaatat tagtgctgtt gtagtattgg gacttatatg
    10081 tattccatat atgcaaaagg gaagccatat tatgaatatt tcttcacaag catcattcca
    10141 gccattgcca tatcaaaata tttatagttc aactaaagcg tttataagaa attatacaag
    10201 agcattaaat gttgaactta agagtaaagg gataaatgca attgctgtat gtccaggttg
    10261 gatgagtaca aatctattta agagaggtat agttgtagct gaaaaaggga caaaaaattt
    10321 cccaggcata gtgtctccag acgttgttgc caaaaaagca ttgaaggatg caaagaaaaa
    10381 taaggatatt tctgtgtatg gtataaatac aaaatccagt catttattag caaaactatt
    10441 gccacaaaaa atgatgatga aggtttggtt aatgcaacaa catattaagg aataagagtt
    10501 tcggtaatta agtatcagtt ggagattcaa tctattatat attataagaa gttttgttga
    10561 gcaaataata attgtgacaa aacggcttat ctagtgccga taagccgtct ataatgtata
    10621 aaaaatatat ttttgatttt actttattta gatattgaag tattaaaata tgtaggactt
    10681 gaaattttat aaagataaat actatgatat tttgtaaaaa ctgagatatg ttgatattac
    10741 atagtttaac tatactgtta aattgaagtt ttcttatttt gcattatgct tattacttaa
    10801 tgatttagac tataatttcc ataccatcat atgcaaaata aatgttatca agtttctttt
    10861 ctaattcact gtaataacca tatgattttc cccacatttc gtcaatatgt gtaatgataa
    10921 tctttttaat tctataatag tttttaattt caacaatctc atctaaagta aatatttcat
    10981 ctttaaatgg tgtgtcttca agttttgtac catcatttaa tataccatca tcagaaacta
    11041 gacttattat taaaacatca gcatcaaagt acatatcact atgagtaaat ggcttgggat
    11101 tacagcaggc atatatgact tttttttcat tctctttgat aacatagaag gttatattcc
    11161 tgtctgggtt attgttaatc aaatcaatat caaaattatt tatctttaca tgcgaagttt
    11221 cattaataga aatgcaattt ataacatcac cataatattt aagacaatcg agattcgttc
    11281 ggtttatatc ttcaattact tcaggaagag cataaaagcc aattggatta ttttctcctg
    11341 tgataaagtc acatcctatt ttttcgacaa ttctaatacc cttaacatgg tctgggtcat
    11401 ggtgagaaat tgaaatatgt tttacttccg atattttttg tctgttacaa gctactgtaa
    11461 tatcttcagg tgtgtctatc agcattttta tatcatcaat atataaactt ggtcctaatc
    11521 gttcatatct tccgcctttt tgcctggctt cttcacatat tttgcattta caaaatgaac
    11581 ttggaataat tgacataccg ccactaccaa caatttttaa tttcaaagtt attacaccac
    11641 cttctaaaaa tattagatta tataaataat ctacaatcac aactgattaa caaaattatg
    11701 agttaataaa ttatatcaca tgtaaatatg aaagaatagt atgaaatttt tataagatta
    11761 tattaaatat ttctcaatga aattgacttt gtatatgagc cgaatgactt tattcaatat
    11821 agataagaca tttttcttgt aataagatat gattattcta taaggttcat gtaattttga
    11881 tataaaatat tgattaataa catatatgtt attggtgaac atatttctat agaatggaat
    11941 atataatttt ttatatttat cattgtatta ttatacaaag aaagttaaaa agagtagagt
    12001 acaaaggaat tatttgtact ttactctttg ttttgttaaa aactaattta tattagtgat
    12061 tcaaatattt aactagagta taagggttgt atataatctt ataagtaatt tatctgacaa
    12121 attaagataa tatcctaatc aaattctagt cattcgaaca taacatcaca agtgatttat
    12181 ctgacaaatt aagataatat cctaattaaa ttgtagatat ttatacataa cactaaaagt
    12241 aatttgttta acaaattaag ataatatcct aattaaattg taggtattta tacataatac
    12301 taaaagtaat ttgtttaaca aattaaaata ttatccaatt aatataaaaa ttacttcttt
    12361 ttaactggga agcatacctc tgttacccaa ttatcaggat tactatcgtt gccaggactt
    12421 acatgataaa tattaaacat aggaccatcc acatcatagt tattatctga tatccaatta
    12481 ccaatagcct cacaagcgtc tgcaacttga ttgaaatttc cattaacaat tgcagttgca
    12541 gcagtcacac ttggaacagt cttaaattta acatgttcag tatctttata ctttccagat
    12601 acagcaactt gtacttcaac atctacagaa tcttctttat atccagtatc ataaaagact
    12661 gctttagaat actttggggg ttcaatttga acattctggt cttttgtctc taaaaatgct
    12721 tgatgccata acattccttc gtcttgataa gttggtataa cccttctaag tgtcatcata
    12781 tattttggtg caaaattctt tatagttaca tcataatttt tcataatatc atttcctcca
    12841 attcttttaa tagtggtttc tatttttaat agttttttct gtgtatcttc taattgttct
    12901 tttacttgag aatggtggat atttagatat tttataagac tttctttatc gttatattct
    12961 gtcaaaattt cttttataga atacaagcca aagcccatat cttttagtgc atggattcta
    13021 tttgtagtag aaagttggtc tgcactatag tatctatatc cattagtgtt attcgtatga
    13081 gaaggagtta aaagtcctat ttcatcataa tgacgtaaca tacgaatact aattttactt
    13141 agctttgaaa aatcgcctat cttaaacatg tttttgtctc ctttataaag gtacttttag
    13201 cttaatatta atataaacca tatcataatg ttagagtcaa ctaaaattat aattaaatta
    13261 tattgacata tgatgaaaaa aatattatac tgaacatgtt cagaacctaa aatgttaatt
    13321 attttggagg tctattatga aaagtaatga taaagatgct agagaacgta taattgaagt
    13381 tacattgaat cttttaaatg aagtagatga tattgaggag attacagtta gaaaaattgc
    13441 tgagcgtgca aatgttggag ttggtttaat aaattatcat tttaaaacaa aggataattt
    13501 gttgagtact gccataggag atgtgatgtc aaatataata gcagagctat atgacgatag
    13561 tgtatatact ctaagaccta ttgaagattt gaaaaattta ttaaaaaaat tgtgtgatac
    13621 tggattacat tatgaaaaag tgcttccttt tgtgttgaat caatgtataa ccaatggaga
    13681 tatgcaagca gaacttgata ttgtccctat gcttcgtaaa atttttggaa ataaaaaaga
    13741 tgaaatgagt ctaagaatca ttgccctgca aattatttta ccaatacaga tttctgcttt
    13801 atctacagaa tcatttcagt tatacagtgg tataaatata aagaataaat atgagcgaga
    13861 taaattcatt gatattttaa tagaaaatat tattggagag gatgttgatg taagatgaaa
    13921 attataacta ttggttcaag tcttatcact gttttacttt ttctttcaac tatggtttgt
    13981 gggttctgga ttaagaataa taaggtaaca gatgcaagtt ctattaagtt ccatatgaat
    14041 agtgctattt ttacagggat atttttattg atttccacaa tccttttaat tatatatata
    14101 aaaaaataat aatattatta ataaataaag tatcagaaat tataaagaca agagtcttaa
    14161 aaatttctga tactttacca cataatttta ttaaacatta attgtgtgtg aaaaaatatc
    14221 acttatttca atctaatact aaatcttaag agtgagtata aagtattaga aattaagaag
    14281 cattttttag ttcaagtgga ttatctatac caaaataatc atataaagta gagcttattt
    14341 cctctaaatt tccatcttgg aaaggtaatt gtaaatttac cttttctacc acatctttaa
    14401 atccagcatc agtattttga tttaaaaaat ccatatatac atcaacagct tttctgtaat
    14461 cttcttgtga taattgccat atctgaagtg atacatcaga tgcaagacca taactcaagt
    14521 aataaaaagg aacttggaag ttatgagata caagtatcca aaaaggagca tttttttctc
    14581 taagtatatt attggaaact ccatattctt cctctaaatc aaaaaacaat ttattgatat
    14641 catttagact catatatgga ttcttgtata taatttcttg aaattcatca taaagacaag
    14701 catcaataat agtatttaaa acaatcgata agtgttcttt ttttatagct tcagattgtt
    14761 taccaaaaaa atcatcaaaa tatttataaa atagaatttc taaagatgtt gaatgaactt
    14821 cacatacatc tatacttggt tgcatcttat tatttaagtt aatactgttg tagtaattat
    14881 aaaaatgacc aaactcatga gcaaaactag taacacctaa aaaagaattg tcccatgtat
    14941 taaacaagaa aggtgcttta tatttattta tataagtagt atatcctcca ggcgatttgt
    15001 tttttccaga agaataatcg tacaagtcat attttttcat atagtcgaag ctctctttta
    15061 gttttggaga tatatcttca agtactttat caaacttcct aaatgagcga tttttatata
    15121 ctttaatata aatagaagaa tcagatggtt tagatgaaat ctctctatat aatggaacta
    15181 tgtaatcttt tacatcttgt ctaaattttt tagcttcttc ttgagaatag tctttgttta
    15241 ggttcatata ggcataatct atataattct taaatccttg cttacttgct atttcagttc
    15301 ttgtctgtat aagttctaag aatatgttac caaatatagg attgtatttt tttaagtaat
    15361 ctgaataaat tttcacatat tcttcagggc taagatttgg tcttgacaag gcttcttcaa
    15421 agtctacctc tataccatct attgatactg tagatttagc taataagtct tcatatttta
    15481 caactaggtc ttgctctttt ttaagtaaat cttcaacttc tttagaatta agttttccaa
    15541 tgttctttag ataattaaat gtatcctttc caagttcttt ttctaattct ttagagaatt
    15601 tagaagttac aaatctatct tgaaagtctt tgtatgacat gtcaacatct actgatttac
    15661 tcataagata cttatattcc ttagagtaaa attcgtctga tgaatctatg ttatttctaa
    15721 tttttgctat agtaagtgta ctgtaaaaat cctgataaat tttatcaact tcattaaata
    15781 aatttaattg ttccttagct gttttagctg ttaaaagttt gtcgttacac ttgttaatgt
    15841 tttcgcaaat tgactgtata ttaggacgct tatattccat tttttcaaaa ggaatagttc
    15901 ctctaacaga atcttttttt gtatcattag tataatttct tatgtatgat gcaaatataa
    15961 taaaaactgc aattaaaatt agaaaaacaa atattttctt cttggattta accataaaaa
    16021 tcctccaatc atggatatag ttattaatta ttttactata ttaaatataa aaaatgcatg
    16081 ttaagaaa
//
LOCUS       DAELSY010000088        15981 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.088, whole genome shotgun sequence.
ACCESSION   DAELSY010000088 DAELSY010000000
VERSION     DAELSY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15981
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.088"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            171..2030
                     /locus_tag="KPG09_002478"
     CDS             171..2030
                     /locus_tag="KPG09_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0967081.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVC
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICINKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEEILDKEFVYNS"
     gene            complement(2322..2921)
                     /locus_tag="KPG09_002479"
     CDS             complement(2322..2921)
                     /locus_tag="KPG09_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0967082.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(3024..3821)
                     /locus_tag="KPG09_002480"
     CDS             complement(3024..3821)
                     /locus_tag="KPG09_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0967083.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3934..4039)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4131..5117)
                     /locus_tag="KPG09_002481"
     CDS             complement(4131..5117)
                     /locus_tag="KPG09_002481"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG0967084.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLNEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5601..7331
                     /locus_tag="KPG09_002482"
     CDS             5601..7331
                     /locus_tag="KPG09_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBG0967085.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7441..7521
                     /locus_tag="KPG09_002483"
     tRNA            7441..7521
                     /locus_tag="KPG09_002483"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7475..7477,aa:Leu,seq:tag)
     gene            7753..7926
                     /locus_tag="KPG09_002484"
                     /pseudo
     CDS             7753..7926
                     /locus_tag="KPG09_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
     regulatory      8418..8522
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            8732..11206
                     /gene="gcvPA"
                     /locus_tag="KPG09_002485"
     CDS             8732..11206
                     /gene="gcvPA"
                     /locus_tag="KPG09_002485"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBG0967086.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISTLKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHHETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11206..12663
                     /gene="gcvPB"
                     /locus_tag="KPG09_002486"
     CDS             11206..12663
                     /gene="gcvPB"
                     /locus_tag="KPG09_002486"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBG0967087.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPLIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            13086..15443
                     /locus_tag="KPG09_002487"
     CDS             13086..15443
                     /locus_tag="KPG09_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBG0967088.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKDDFLYSGSFVVSGEAFVRVENIGKDNYANKIVNDAKISKKHNSQLRNSLN
                     SILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKIRIEVEKYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDNDIYEAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(15529..15846)
                     /locus_tag="KPG09_002488"
     CDS             complement(15529..15846)
                     /locus_tag="KPG09_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBG0967089.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         6023 a         1871 c         2619 g         5468 t
ORIGIN      
        1 aaaaaaattt ttgtatatat aaaggactct aaagatgaac tatataaaac aaggtttata
       61 tattctaata gtatagaaaa ttttattgta aacgcatgta tattattaga aaaaatagtt
      121 tgaaggtatt caataaacat aaaaaacaat ataggggata aaggtgtttt atgaagagat
      181 ttttgaggag aattatctta gttttattta ttttgttgat ttttataagt tttatttcta
      241 ttaaattaat tcataatgtt ggagattatg gaaaattgat taattatgtt gggattgttt
      301 gtggagctag ccaacgtcta actaaactag agatgaatca tcaaccaaat gatgaattaa
      361 tagaatatat tgatgaaatc ctacaggagt tgattacagg gcatggagat tatggtttag
      421 taatcactga ttgtaatgaa tacaatgagg acttactttt attagaaaaa aagtgggaag
      481 atttaaatat tgaaattaaa aaagtaagaa tgaaagaaca gaataaccag ttattaagta
      541 taagtgaaga gtttttttct cttgcaaatg atacagtgtt taaaatagaa aacttttcta
      601 aagaaaaaag taattactta atgactttaa taataattat atctattata ggtatattag
      661 catgtatcat attgatttta caatatagta aaaaaatggt tagactggaa aaattaaatg
      721 tagatttaaa aaatattgca tataaagatg aactaacagg agtaaatact atagaaaaat
      781 ttaaattgga tgcaaatcaa aatatttgta taaataagga taagaaattt gctgtattct
      841 atattgattt tgaaaacttt aaatacatta atgatatatt tggatatgat tatggtgaca
      901 tgatactgaa aagatatgct aatcttatga tgaatgacat agggaaatat gaaatatttg
      961 caagagaaat tgcagataga tttgttgcgt taagatgcta tgtagataag gaagatttag
     1021 tagtaagaca acgagatgta gacaatgaac taattaatat aacaaatgaa attaaaaata
     1081 aacatagtat tactgttgtc agtggaattt gttgcattga agatgtaaat gaaaaattaa
     1141 gtatagatgg tttaataaat agagcaaatt ttgcacaaaa aactgttaag aataaaccag
     1201 gtactaatta tgctttttat aatgacagta ttcgtaaaaa aatgattgaa gaaaatacta
     1261 tcaagagtag aatacatgaa gctattgaaa aaagagaatt tatagtttat ttacagccta
     1321 aagttaattt acatagtcaa aaaataaatt gtgcagaagc tttagttcgt tggcttactc
     1381 cggataaagg tattatatct ccagcaatat ttattccagt acttgagaag aattttttta
     1441 ttgccttagt agataagtat gtttttgagg aggtttgtaa atggattaga aagaggttag
     1501 atgaaaataa accttttgtt cagatatcag ttaatgtttc aagaatacaa ttttataaca
     1561 ctaaatttgt agaaacttat tccaatatac aaaataaata caggatacct aaaaatacta
     1621 tagaaattga atttacagag agtgttgctt ttgagaatca aaaacatctt ttggaaatta
     1681 tacatgattt gcatgaaaat ggatttactt gttctttaga tgattttgga aaaggttatt
     1741 cgtctttaag tgttttaaaa gatttaccat ttgatgtttt aaagcttgac tctatgtttt
     1801 tcaaggctag tttagataaa gataaagaga aaatagtaat aaaaaatatt gtacatatgt
     1861 taaaagagct taatatcaca actgttgctg agggcattga atgcaaggaa caagttgaat
     1921 ttcttaggga tattgggtgt gatttagttc agggatttgt attttataaa ccaatgccaa
     1981 tattagaatt tgaagaaata ttggataaag agtttgttta taatagctaa aatgtacttt
     2041 ataaaatatg aactaattaa gtaaaattat tttgtactga ataaattttt actttattta
     2101 cagaatagtg attatttaca gaaaagggcg aaaagtgtat tcctttaagt atgggatgga
     2161 tagcacaaat aggggagaaa aaatattggc catataagaa tgcataataa tataaatatt
     2221 tgaaaggtca gcattatata aaaattaaaa agaaaataaa taatttttct ataaaaaaga
     2281 gactatgaga aaagttactc atagtctcct aaataatgta tttattttct tcctgcaacc
     2341 cattttaagc cccaatgaaa ttcagtatcc atttctctat gacctttgaa gtatttaact
     2401 actttttcaa cactgaatgt ctcatcattt acattttcta tcaatgctat tgaacacata
     2461 ttatagccat ttttatgttc atcaagtttg acaactaagt ctgaatcttg tttttgtttg
     2521 attgttacaa ttccatcagc ttcagaccag ttagctacac cttcgtatat aaaagcataa
     2581 ataagtattc tttttatatc ttttatttta ttaccattta ttcttaaatt ttctccttgt
     2641 gtattacttc ctgttctgtc atctccatct aattgagcaa aaggtggatt gtctaaacta
     2701 ccaaaagcat ttcctaatgc ttgaacagca ccttttacac catttttcat ctcaaatagg
     2761 caacccaaat ctaaatctat attactgctt cttaaagagc caaaaaaacc ctttttttgt
     2821 actttttgat tccaatttaa attaactaag atttcaccta aatcgctatt ttcctttttt
     2881 gtaaggttta ttttttgtcc cttttttaat tctatagcca tagtaatctt cctccacaaa
     2941 catagattta tttaaatggt agcacttaat aagtcaatat tcaacaagat tagaataata
     3001 atattaaaac tatactaaat atactaaaaa gtaggaatca caataccttt gtatttttct
     3061 tcaattaagc ttcttgcttt atctgatgta agaacatctt gtagaacttt aatcttttca
     3121 ctgtctttat cttcagttct aaatgctaaa agatttgcat actcgtcatt tataggagct
     3181 gtataaattc cgtttttctt ggcatcaaga ccagcaggta cagcataagt agtatctata
     3241 aatgcagcag taacatctgg aagagaagat ggaatttgag caatatctaa ctctttgaat
     3301 tgtattccct ttggattttc tattatgtcc ttaggactaa ctaatccatc ccctttaggt
     3361 aacgttaaaa gtccaatact ttcaaggtat ctaaggttct tagagcaaga agatgggtta
     3421 ttgcttattg caattgtatc tcctttttta aattcatcaa cagattttat cttataagag
     3481 taaagtattg ctgggcatac atatagtttt ttaccagcca ttattttgta acctttggcc
     3541 ttaacagctt cttttagata aggttcatgt tgaaatagat ttgcatcaat ttcaccatta
     3601 tttaaagctt cgtttggaag tatgtaatca tcaaagtttt taacttctaa agtatatcct
     3661 ttttctttaa taagtggttt taattcttct aataactctc ctcctggaac taaagtagca
     3721 ccaacaacaa tttttttatc atctttagct ttagaacatc caaccacagc taaagttagt
     3781 actagacata ataacaaaca taataatttt ttgaatttca tataaattcc ctcctgaata
     3841 taattgatta gttaaaaata aaataccatt ctttatcaca ctgcaaaata aaaataagcc
     3901 tgagtttaaa ctcaggcttt aaaagctaat ttagactcat ctttcagtat aatactgcag
     3961 gatttagcac caatgtatat aaaaatatac tggttgctgg gtttcatagg gccagtccct
     4021 tcaccactct ggataagaag tatcaatttt taattatgtt ataaatatta tcatgatttt
     4081 ttacgtctgt aaacataatt ttataaatat tttaaaatct ttaatgtatt ttatttaaca
     4141 cctaaaattc cagaaactag aatatcagta tcagcaccta agaaatagtc atttatttca
     4201 atagaatcca gagtatttct caaagcattt tcttcatgcc ttataccaat aagtttattt
     4261 tctacaaatg caacatcaca ttttccaaaa aaatctccaa aaaattttat actagttatt
     4321 atgcctttat caacatttaa attaaactca acatttccac cagaatattt taattcattt
     4381 gaaagagtat attttggtga attaccaaaa ttccaatccc aagtttcgta ttttgattta
     4441 gtaagctttt ctatttgttt tatatcatgc tcattcaaac aataattagt attatcagta
     4501 tttgttttag aaagataatc cattatagca tctttaaatt ctaatatgtc catttttgaa
     4561 tgtaaatgtt cacttatatt ggttacacga gattttacag attttattcc ttttccttca
     4621 aattttatag gtttaacctt taatgctttt gagaggtcat ttatttcaga tgaaaataaa
     4681 agagtaccat gatgcattac tttgtttttg tagttgtatt gagcatttcc agaaaatttc
     4741 ttgccatcta taagaaggtc atttcttcca gaaaattcgg cattgacatc tagtgtttta
     4801 agtaaatcta ttatgggctg agtgaatctt ctgaaatcac taaaattatt attgttacaa
     4861 gctataaatg taaaatttat atttcccaaa tcatgaaata cagctccacc accagactgt
     4921 ctcctaacga caggtataga atattctttt atatattcta aatttatttc agaaagtgtg
     4981 ttttggtttt ttccaacaat tatagaaggt tcattcctcc ataaaataaa taagtcttca
     5041 tcaaaatttt ttagaagata ttcttccatt gctaaattaa aataagggtt tgttttttca
     5101 ttataaatta ataacattaa agtagttcct ccatagaatt gataaaattt ttattatgaa
     5161 tacaattaat tttataattc gacaataata taaaaactcc tttaaattag aaattttaaa
     5221 aactaccttg caaagtttaa ataaataata tatactcaaa atatgtttaa taaaagtgaa
     5281 tagaagggta gaggcgcaat aatctatcag tactaataag gagttaagca cagagagtta
     5341 ttaggaaagg agatattgcc gaagttttat aatcgatgct ttaagattat attgctgggg
     5401 gtacatagaa aatatgtaca actgtcacag gtttatttgt ggtgagctat catttaagga
     5461 attatattat atttattttt actattgttg atatattgta atgtttaagc cttgagtgtt
     5521 taccactcaa ggctttttta gttttattgt aacataatac cctgtacagt tcacaaaatt
     5581 aaaaggagag ggagatttat atgaggaaca aaaaaattaa tattattttt ctgacgacaa
     5641 tcatgttcat aatgtcaaca gtaatggttt ttgcagaaga agatatagac actattgcac
     5701 tagctaatgc agaaaaattt gggattttaa ctttaatacc accattagtt gctataatac
     5761 ttgcatttat aactaaaaac gtaataattt ctttacttat aggtattctt tctggtagtt
     5821 ttataattaa agctagtggt attaatgttt ttgcgacatt tatacaggca tttttagatt
     5881 tagtagatag agctttagta tctttagcag acccttggaa tgcagggata atactacagg
     5941 ttttggcaat tggaggagta ataaatcttg ttgctaaaat gggaggagct aaagctatag
     6001 ctgaagcact tgctaaaaga gcaaagtcag caaaagggac acagcttata acatggtttt
     6061 tggggctttt agtattcttt gatgattatg ccaattcact aatagtaggt cctatgatga
     6121 gacctgttgc agataaaatg aaaatatcaa gagaaaagct agcatttata atagatgcca
     6181 cagcagcacc agttgctggt ttagctataa tatctacatg gataggtctt gaggttggtt
     6241 taatacatga tgcttttgaa agtataagta tagatgtaga tgcatttggt atatttttaa
     6301 atacaatacc atttagattt tacaatatat taatattagc tttcattgta atatcagcat
     6361 tattattaaa agaatttggt ccaatgagaa aagctgagat aaaatctaga agtagaaaaa
     6421 ttagtataga ccttgatgaa ggcgtagaag agttggatga tttagctcca aaaaatggag
     6481 taaaattgag tgtatggaat gctataatcc caataggaac attgataata gttgcattag
     6541 catcatttta ttatagtgga tatacatcta taatgggagg agacgataag gctctgatac
     6601 aactatttac aaattctcca tattcttttg aagcaataaa agaagcattt agtgcatctg
     6661 atgcatctag agcattattt cagtctgcac ttgttgcaag tttagttgct attataatgg
     6721 ctgtagtaaa aaagattttt acaatatcag aagcaattga tgtttggata gatggaatga
     6781 aaagtttagt aatcactggt gtaatattaa tacttgcatg gtcattaagt tcagtaataa
     6841 aagagttagg tacagctaag tttttaatac acctattatc aggttcacta ccaccattct
     6901 tattaccaag tttaatattt ggtttaggag caataatatc ttttgctact ggaacagctt
     6961 atggaacaat gggtatactt atgccacttg caattccatt agcatactca ttaaatcctg
     7021 atatgtctta tgtaatagta agtactagtg cagttttaac aggtgcaata tttggagacc
     7081 attgttctcc aatttcagat acaacaatac tttcttcaat gggagctggc tgtaatcata
     7141 tagaccatgt taacactcaa atgccatatg ctatatttac agcagtaatc actattgtat
     7201 ttgggtatat accagctgga cttggattgc caatatatat agtattacca gtggcaatag
     7261 ctgctatatt tgtaggtatt caaataattg gtaagaaagt tgatgaagcg gaaatagaac
     7321 ttgtagaata aagatataaa taagtttcac cttttattat cactattttg ttagttttta
     7381 gataatttta aaaagttatt gaaaataatg gcgaaatata gtaaaataaa agagtgataa
     7441 gcggatgtgg cggaattggc agacgcacta gacttaggat ctagcgccat tggcgtgggg
     7501 gttcgactcc cttcatccgc actttacagt acttaattgt actgaaaaaa actggggttg
     7561 ctggctttcg gagtgggcta tcccgttttt atttttttgt atatataata gattggcact
     7621 ttagtataag gtgctttttt tataggcaaa aacagtacat ttatttctgt tttattatca
     7681 ttcatctatt agaatatact ttatttaaga atgtgttaat aagcagtgtt cacattcttg
     7741 ataaaatgtg gaataatatg cctaccaata agcatttggt gtataagtaa gagtatttat
     7801 atagttgatt gtgaaatgag tacaacaatt ctgtacagta taattggcat tataattgta
     7861 gcattgaatt taaagtttgc acagtcgctg attggaaaat ttacaaagtg gatgtcaaac
     7921 atataaattt tagtttattg gacagttcaa ggaatcagaa attggaattt tatagtatta
     7981 atattttata gtattaatat aatgtataaa attttatagc aatgtatttc ataagtataa
     8041 agtgactaga attttaagaa atatacaaat tctataaaaa ctaaataatt tcaagtatat
     8101 tttaaaaatc ttatttttca caagtttaaa attgtaaacg atttcatcaa gtctatgaat
     8161 atacttttaa catgttatag taaatctcaa tataaattta catttggttc tttgtgtgtt
     8221 attttaataa taaattaagt gaaaatgtta ttttacatta atatataata tctttgcata
     8281 agagtgaata atataaaaga gaaagaattg ataataaaaa cttaacaaaa aaattaatac
     8341 ataaaaaaaa ataaatataa tgtgttgaca tttaattgaa atattcatac aataaaattg
     8401 aaaataaata tttccaaatg agattagcga gagagtcctt tatacgaaaa ggcgccgaag
     8461 aagaaattta agaaatgcca atttttagag aagctttcag gcaaaaatat cgctaattga
     8521 tgggactctg gagagactta agttaagata ctataagtca ccgaagaagc aaaataatat
     8581 taaattatta aatctttcag gtatacaaac agggatatat aggctaggat tattttccat
     8641 gctgatatat ctttttttta tgaaaaagta caggaaataa tatcaaagtt acttaaatac
     8701 agtaggtcta attttctagg gagggttata tatgaatgaa ttgaaaagag tatctttgta
     8761 caatatacac aaagagcttg gagcaaaact tgttgaattt gctggctggg aaatgccttt
     8821 agaatatgag ggtataaata aagagcatga gaaggttaga aaaagtgcag gtatatttga
     8881 tgtatctcat atgggagagg tacagataaa gggagcagag tcagaaaaat ttatacaaaa
     8941 cctagttaca aatgatataa gcacattaaa gataaatgac attatatata cacctatgtg
     9001 ttatgaaaat ggtggagtag ttgatgattt attaatatat aaatttggtg aagaagatta
     9061 tttattagta ataaatgcag gtaatataga taaagatgta gcatggataa taaaacaaag
     9121 tgaaggatac aatgtagata taaaaaatat atctagtgaa gtgagtcaat tagcaattca
     9181 aggacctaaa gcagaagaaa ttcttcaaaa aatcacagat atagacttaa actcaataaa
     9241 attttataag tcaattccat caacaaaagt atgtggttgt ccttgtttag tttcaagaac
     9301 aggatatact ggggaagatg ggtttgagat atactgtaaa aataaatatg tagaaataat
     9361 ttggaatgaa gttctaaagg ttggaggaga agatatatgt ccagctggtc taggttgtag
     9421 ggatactttg agatttgaag cagctctacc tctatatggt catgaaataa atgaacacat
     9481 atctccaatt gaaggtggtt tatctatatt tgtgaaaaca aataaagaat catttattgg
     9541 taaatcaatt ttatcaaaag aaaaagaaag tggagctaaa agaaagttag taggatttga
     9601 aatgcaaggt aaaggaatgc ctagaaatgg atatgatata aggattggtg ataaaacagt
     9661 tggatttgta acaacaggat gtgcatctcc aacgacagga aaaattttag gtatgggaat
     9721 tatagattct gaatatgcaa aagtaggaaa tgaaataggt atagcaatca gaaaaaaagt
     9781 tgttcctgct gtgatagtta agaaaccttt ttataaaaaa caatataaaa aagataatat
     9841 tatattaaac aaagaaaata aattttcata tatacctgct acaagtgaag ataaatcaaa
     9901 aatgttaaaa gtagttggat taaattcggt tgatgaatta ttttcagata taccagaaga
     9961 agtaaaatta aaaagagatt taaatttgga aattggaaaa tctgaattag aggtaagcaa
    10021 aatagtaaaa agattgtcag aagaaaactt aagtttagaa gacttgactt gttttcttgg
    10081 tgctggtgca tatgaccatt atataccatc aattataaaa catataactt caagatctga
    10141 attttataca gcatacactc catatcaagc tgaaataagt cagggaactt tacaagtagt
    10201 ttttgaattt caatctatga ttgcagaaat tacaggtatg gaaattgcaa atgcatcaat
    10261 gtatgatgga gcaactgctg caatagaagc atgtataatg gcaatgaatc agactagaaa
    10321 aagtaaaata gtagtgtcaa aaactattca tcatgaaact ttgtcagtat taagaactta
    10381 cttacaatat aaagattgtg agatagtaga aattgacttt tgtaatgaat atgggactac
    10441 agatatagaa aaattaaaag cttctgtaga caaggataca gcatgtgttc ttatacaaac
    10501 tcctaatttc tttggaatta ttgaagaaat ggaagaaata gaaaagataa ctcatgaaaa
    10561 taaggctatg ttaattatga gtgtagaccc aatatcatta ggagttttaa aaacaccagg
    10621 tgagataggt gcagatattg ttgttggaga agctcaatca cttggaaacc ctcttaattt
    10681 tggaggtcct tatgtaggat ttttagctag caaatctaaa tatactagaa aaatgccagg
    10741 aagaatagtt gggcaaagtt tagatgtaga aggtaaaatt gcatatgtat taactttaca
    10801 aactagagaa cagcatgtaa gaagagaaaa agcaacttct aatatatgtt caaatcaagc
    10861 tttaaatgct cttgtagctt caatatatat ggctactatg ggtaaagaag gatttaaaga
    10921 ggttggtatg cagtctatga aaaaagcaca ttatacttac aataagcttg tacaaactgg
    10981 aaaatataag ccaatattta aaggtaagtt tttcaaagaa tttgctgtac aaggaaatct
    11041 taatatagaa actataaatg ataagctctt agaagaaaat atattaggtg gttataattt
    11101 agaatacaat tatccagaat taaaaaattc aactttgctt tgtgtgactg agaaaagaag
    11161 caaagaggaa atagataagt tagttggaat aatggaggga ttataatgaa agaatacaat
    11221 agtttgttga tagatatatc taaaaaaggt aggaaagcat actcacttcc aaagttagat
    11281 atagatgata taaaaataga agatatgatt gaccaagata tggcaagaca aagtgagttg
    11341 aatttgccag aagttggtga attagaatta gttagacatt atacattatt atcaaataaa
    11401 aactttggag ttgatacagg tttttatcct ttgggttctt gtactatgaa atataatcct
    11461 aaaataaatg aagatatggc agcacttcca aactttactg gaatgcatcc ataccaatct
    11521 tcggatacag ctcaaggttc tctttctttg atgtatgatt tatcaagaag acttgcagaa
    11581 attacaggta tggatgaagt tacactacag ccttcagcag gttctcatgg tgagtttaca
    11641 gggcttatga ttataaaagc atatcatgaa aatagaggtg accataagag aactaaagtg
    11701 ataattccag attctgcaca tggaacaaat ccagcaagtg cagctatggc aaactttgat
    11761 gtgattcaaa tagcatctga taaaaatgga gctgtagaca taaatgcatt aaaagaggtt
    11821 ttgaatgatg aagtagcagc tcttatgctt actaatccaa gtactcttgg tttatttgaa
    11881 aaaaatataa aagaaatagc tactcttgta catgaagcag gaggactttt gtactatgat
    11941 ggtgcaaaca tgaatgctat tatggggata acaagacctg gagatatggg gtttgatgtg
    12001 gttcatctta atcttcataa gactttttca actcctcatg gaggtggagg tccaggagct
    12061 ggtcctgttg gagttaaaaa agaattagta ccttttttac caataccatt aattgaaaga
    12121 aaagaagata agtatgtact aaattatgat agagaaaaat ctattgggaa aattaaaaat
    12181 ttctatggta attttggagt tcttgtaaga gcttacacat atatactaac tatgggaagg
    12241 gatggactta aagaggctag tgaaatggca gttttaaatg ctaattatat aaaggaaagt
    12301 ataaaagatg attatatttt gccaatagat accttgtgta agcatgagtt tgtattaggt
    12361 ggattgccaa gaggagaggc caatataaaa actatcgata ttgcaaaaag actactagat
    12421 tatggatacc atcctccaac aatgtacttt cctcttatta taaatgaggc acttatgata
    12481 gaacctacag aaagtgagag tatagagaca ttagatagtt ttatagaagc tatgaaaagt
    12541 gttgctaaag aggcaaagga agaaccagaa ctattgaaaa ctgctccaca taatactttg
    12601 gtcaaaagag tagatgatgc aagggcagta aagaaaccta tactaacatg gtcacaaaga
    12661 taaataatta aaaagtagag atttaaagta taaagagaat taaatttgta agaaaaagct
    12721 atctaactta atccagttta gatagctttt ttgtatatta gtatgtatgg tattttctat
    12781 ataaagtttt aaattatatt agtgcttata tagaatattg atatgatgat tatgtataga
    12841 tattaataag aattgttgaa tttatcttgc cttaacttta tttactcttt tatcttaaga
    12901 aaaacttaac taaaatatag cgaaattcat aaaaatttag gaaatcttaa gaattataat
    12961 atttaaatct ttagatttat attttattat ataaactata gaataataaa atataaaaat
    13021 tgagaatttt ataatatatt tgtagatttg agtataaaaa agtgctaagt tgaaaggagt
    13081 aaaatatgga taaggttaat ttagaaaggt atagttgtga tataaatgtt ggtttaaata
    13141 aaaatcaact tcaaagtaga atagatgata atttaattaa tgcatttgat aatggaaaaa
    13201 ccaaaacata taaacaaatt tttaaagaca atatatttac actttttaat ctcataaata
    13261 ttgttttagt ctgtttatta gtttcagttg gttcttttaa aaatacctta tttatattta
    13321 ttgctgtaat taatactatt ataggagtaa tacaagagat aagagctaaa agattattgg
    13381 acaatctatc tgttatagtt tcaaataagg tatctgtcat aagagaaaaa gagaaaaaag
    13441 agattgatgt acaagaatta gtattagatg atattatgat tttaaaaaca ggtaatcaaa
    13501 tttgtgcaga ttcaaaagtt ttaaatggaa aattagaggt aaatgagtct ttggttacag
    13561 gtgaatctga tattattata aaaaataaag acgatttttt atattctgga agttttgtag
    13621 tttctggaga agcttttgta agagttgaaa atataggaaa agataactat gcaaataaga
    13681 tagttaatga tgcaaagatt tctaaaaaac ataattctca acttagaaat tcattaaatt
    13741 caatattaaa aattgtaggt ataataataa ttcctttagg aattatattg tttgcaaaac
    13801 agcattttgg aaatggagat tctatagcta catctgtagt aggtacagtg gcagctgtaa
    13861 atggtatgat accagaggga ttaacattac ttacgagcat tgctctagct gtaggaacta
    13921 taaaacttgc tagtcataaa actttagtgc aagaactgta ctgtgtagag actcttgcta
    13981 gagttgatac actatgtctt gataagacag gtacaattac agaaggtaaa atgcaagttg
    14041 atgatatagt aatgttggaa gaagtggata ttaatgaaat aatggggaat atatgtaatt
    14101 ctctcaaaga tgataatgca acactaaatg caataagaga taagtataat gttttagata
    14161 cgtataaagc taagaattta ataccatttt cttcagaaag aaaatacagt ggggttacct
    14221 ttgaaaataa aggtacatat ttattaggtg catttgagtt tatatttaag gaaaaaaata
    14281 aaaaaattag aatagaagtt gaaaaatatt ctaaaaaagg aaacagagta atagttttag
    14341 ctcatagtga ttcttatatg gaagataata atattcctga agatataaag ccactagctt
    14401 ttatattgat attagataaa attagagatg aagctaagga aactttagaa tttttttaca
    14461 atgaaggtgt agatataaaa attatatctg gtgacaatcc tataacagta agtgaggtag
    14521 ctagaaaagc aggtcttaaa acggctagta attttattga tgcaacaaca cttaaaaatg
    14581 ataacgatat atacgaagca gttgataaat acagtgtttt tggaagggta agcccacaac
    14641 agaaaaaaca aatgatttta gcattaaaaa aacaaaatca tacagtagct atgactggag
    14701 atggtgtaaa tgatgtgcta gcgcttaaag aagcagattg cagcattgcc atggcatctg
    14761 gaagtgatgt tactaagaat gtttctaatt tagtcttatt agattcaaac tttgcatcaa
    14821 tgccaaaggt tgttatggaa ggacgaaagg taattaataa cattcaaaga gcatcttctt
    14881 tgtttttagt aaagacaatt ttttcattct tcttgtcact attaacatta ttcttcttca
    14941 gtagacagta tccatttgtt cctatacaat tatcactaat tggagctgta acagtgggta
    15001 taccatcatt cttcttagct cttgaagcaa acaaaagtag aatttcagga aactttttac
    15061 tcaatatatt aaaaaatgct ttaccaggag cactatgtgt gacagtaaat gtaattattg
    15121 tttacaatat agttgaatat ttaggctata gttcaagtgt attttctaca atgtgtgcta
    15181 tattaactgg tgtatgtggt cttttaatat taagacaaca atgtttgcca tttaataaag
    15241 aaagattatt tttaattgta agtatgacta ttgcatttgt tattgggatt ctatttttag
    15301 gtggattatt ctcatttgta gcattgagta gagaattaat tctcataact atggcattag
    15361 caatattaat gccaattatg ataaaagtta tgttatttgt cttggatgag atattagaga
    15421 caattgttcc agatttaaat taattttaaa atgatataaa taagagcagt tttagttgaa
    15481 ctgctctttt tagatgtgtt taattttata taagaatatg tatttttatt aatcagctat
    15541 atatgagcaa cagtagtcaa caacttcatt aacctttaaa tcaaaacttg aatcactagg
    15601 aacattaaat ttttgccctt ctttataagt tacaaactca ttgcttccag gcagttttac
    15661 atccatactt ccagcaagca tctccattat ttcttcttct ctagtagaaa aagtatattc
    15721 tcctggcaac attaatccta aagtttttct ttctccatta ggcagtatta ttactctgct
    15781 acttacctta ccatcaaaat aaacgtttgc cttctttact gcagtaacat tcttaaattc
    15841 actcattaga taaaacctcc aaaattattt gtacatatat attatataaa aataatgtaa
    15901 aaaatgctat atatttgcaa aaaaaattta gtatttatta aaaaaattaa taagaataaa
    15961 aaagagatga aattgcttaa a
//
LOCUS       DAELSY010000089        15959 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.089, whole genome shotgun sequence.
ACCESSION   DAELSY010000089 DAELSY010000000
VERSION     DAELSY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15959
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.089"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            235..384
                     /gene="rpmG"
                     /locus_tag="KPG09_002489"
     CDS             235..384
                     /gene="rpmG"
                     /locus_tag="KPG09_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBG0967090.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            410..631
                     /gene="secE"
                     /locus_tag="KPG09_002490"
     CDS             410..631
                     /gene="secE"
                     /locus_tag="KPG09_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBG0967091.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            651..1193
                     /gene="nusG"
                     /locus_tag="KPG09_002491"
     CDS             651..1193
                     /gene="nusG"
                     /locus_tag="KPG09_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBG0967092.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDVDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1229..1654
                     /gene="rplK"
                     /locus_tag="KPG09_002492"
     CDS             1229..1654
                     /gene="rplK"
                     /locus_tag="KPG09_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBG0967093.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1724..2422
                     /gene="rplA"
                     /locus_tag="KPG09_002493"
     CDS             1724..2422
                     /gene="rplA"
                     /locus_tag="KPG09_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBG0967094.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2474..2602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2645..3151
                     /locus_tag="KPG09_002494"
     CDS             2645..3151
                     /locus_tag="KPG09_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBG0967095.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3209..3574
                     /gene="rplL"
                     /locus_tag="KPG09_002495"
     CDS             3209..3574
                     /gene="rplL"
                     /locus_tag="KPG09_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBG0967096.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            3966..4754
                     /locus_tag="KPG09_002496"
     CDS             3966..4754
                     /locus_tag="KPG09_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG0967097.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5225..8941
                     /locus_tag="KPG09_002497"
     CDS             5225..8941
                     /locus_tag="KPG09_002497"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBG0967098.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGQSFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     VEATEDEDIENLEEFTEDDLFEEEIDFESDDFDM"
     gene            8983..12468
                     /gene="rpoC"
                     /locus_tag="KPG09_002498"
     CDS             8983..12468
                     /gene="rpoC"
                     /locus_tag="KPG09_002498"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBG0967099.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVLVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            12757..13179
                     /gene="rpsL"
                     /locus_tag="KPG09_002499"
     CDS             12757..13179
                     /gene="rpsL"
                     /locus_tag="KPG09_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBG0967100.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13302..13772
                     /gene="rpsG"
                     /locus_tag="KPG09_002500"
     CDS             13302..13772
                     /gene="rpsG"
                     /locus_tag="KPG09_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBG0967101.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            13821..15887
                     /gene="fusA"
                     /locus_tag="KPG09_002501"
     CDS             13821..15887
                     /gene="fusA"
                     /locus_tag="KPG09_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG0967102.1"
                     /translation="MARKFPLEKTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFINKMDILGADFYNVISMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6246 a         1985 c         3249 g         4479 t
ORIGIN      
        1 atggattaaa aggaataaca aaaagagggg ttaggctcct ctttttttaa catagaaata
       61 caaaaacatt gacacagcat aggattttgt gataacttat acaaggtaat gattatagga
      121 agttaataat atcttgaata ctgtagattt ttaataaata catattttaa agatattata
      181 ttcacatata ttaacaatat atattattgt attacaaagt tggaggtgta tcagatgaga
      241 gttaaagtaa ctttagcatg tacagagtgt aagcaaagaa actacaacac tactaagaat
      301 aagaaaaata atccagacag aatagaatta caaaaatatt gtagattctg taaaaagcat
      361 acaactcata aagaaacaaa atagtttttt ataaggatgt gagtatggaa tggctgccca
      421 aaaaaatgac ggtgcaaaaa ctaaaaaaag gtttagttta tttggatatt taaaagaaac
      481 taagcaagaa ttaaaaagag ttacatggcc gacaaaaaag gaactgttta aaaacacaag
      541 tatagtttta actgtagtta tttcatgcac tatattagta tggggtatag acactatatt
      601 atctggtgca ctagcgttat tactaaaata gacgaaaaga ggtaagttaa atgtcagaat
      661 tacaagaagc aagttggtat gtagttcata cctattcggg acatgaaaat aaagttaaag
      721 ctacaattga gaaagcagtt aaaacaagag gtatggaaga ctgcataaga caagtagttg
      781 ttccaacaga agaggtagtg gaaactacta aaactggaaa agaaaaaact agacaacgta
      841 aagtttatcc aagttatgtc ttggttaaaa tgataataac tgatgaatct tggtatgtag
      901 taagaaatac taagggagtt acaggtttcg ttggacctgg ttcaaaacca gtaccattaa
      961 gtgaagatga agtcaaagct atggggatag atacaacaga ccctaaagta gtaaatagcg
     1021 atgttgattt tgaaatagga gacacagtaa aagtatctca aggaccattt agtggacaaa
     1081 taggtaacat agaagaaatt gacttagaaa atagagaagt caaagtatgc ataaatgcat
     1141 ttggtaaaag gactctattt gtaatagagc ttgaaggtat agaaaaaatc taatactata
     1201 tagaaaaatc tgaggaggtg cgctcaaaat ggctaaaaaa gttataggtc aaataaaatt
     1261 acaaatacct gcaggaaagg ctactccagc gccaccagtt ggaccagcat taggacaaca
     1321 tggtgttaat ataatgggat tcacaaaaga atttaatgct aaaactgcag atcaagctgg
     1381 aatgataata ccagttgtta taactgtata tcaagataga tcattcagtt ttataacaaa
     1441 aactccacca gctgcagttt taattaaaaa agcattaaac ttaaaatcag gttctggaga
     1501 accaaacaaa aagaaagttg ctaaaatgac ttcagctcaa gtaagagaaa tagctgagtt
     1561 aaaaatgcct gacttaaatg ctgcatcagt agaagctgct atgagtatga tagctggtac
     1621 tgctagaagc atgggtgttg taatcgaaga ctaattaaaa gatacacttt ttttataaag
     1681 tgggaggcga gaagccgaaa ttaaccacaa ggaggaaaat aaaatggcta aaaaaggtaa
     1741 aagatatgca ggtgcattac aaaaagtaga tagaactaaa ttttatgatg catcagaagc
     1801 attaacatta gtttcagata tagctggagc taaatttgat gagacagtag aagcacacat
     1861 taaattaggt gttgactcaa gacatgctga ccaacaagta agaggtgcag ttgtattacc
     1921 tcatggaact ggtaaaacaa aaagagtttt agtttttgct aaaggtgaaa aagctaaaga
     1981 agctgaacaa gctggtgctg actttgtagg agctgaagaa ttagttcaaa aaatacaagg
     2041 tgaaaactgg tttgactttg atatagtagt tgctactcca gatatgatgg gtgtagtagg
     2101 tagattaggt agagtattag gacctaaagg tttaatgcca aaccctaaat caggaacagt
     2161 tacatttgat gtagctaaag ctatagatga aataaaagct ggtaaagttg aatacagatt
     2221 agacaaaact aacataatac acgttccagt aggaaaagta tcatttggtg gagaaaaatt
     2281 aactgaaaac tttactgcat taatggatgc tataataaaa gctaaaccag ctgctgctaa
     2341 aggacaatat ttaagaagta taacagtagc ttcttcaatg ggacctggag ttaaaataaa
     2401 cccagcaaga acagctgaat aataaaactg ttgacacagg ttgacaaaaa tgttattata
     2461 tcacttgtca atgtaaaaaa gaatagattt gccgtagata gtgggtgcga aagcttaata
     2521 tcccgccgag gttttagata aattgagatt actaaagctt ttgatgtcta cttttggcaa
     2581 caaaagcttt ttataatgtt gcggtaaata ttaagcctat ttggtgaagg aggtgcacat
     2641 ctatatgaga aaagctatag aaattaaatc agaagttgtt tctgagatag ttgaaaagtt
     2701 acaaaaatct tctgctgctg ttgttgtaga ttacaaagga ttaacagttg aagaagtaac
     2761 tgagttaaga aaacaaatga gagaagctgg tgtagattac aaagtataca aaaatacttt
     2821 agttagaaga gcagctaaag aagttggtat agagcaattt aatgatgaat tattagttgg
     2881 aactaatgcc atagcatttg gatatgatga tccagttgct ccagctagaa tattaaaagg
     2941 attcatggat tctcatccaa aaatgaaatt aaagatgggt attgtggaag gtgcattcta
     3001 tgatgaatct aagattgttg aaatggctaa cataccatca agagaagttc ttatcgctaa
     3061 attacttggt agcttaaaag ctccagtatc aaacttcgca tacttaatag atgcaatagc
     3121 gaaaaaggca gaaggtcaag aagaagctta attgcttaac taaaaaatta taaatattaa
     3181 tttgaaataa aattcggagg tgctaaaaat gacaatagaa caaatattag aagctataga
     3241 aaatatgaaa gttttagaat taaatgaatt agttaaagct gctgaagaaa aattcggtgt
     3301 atcagcttca gctccagtaa tggtagctgg tgcagctgct ggtggaccag ctgctgaaga
     3361 aaaaactgag tttgatgtag tattaactga cgttggttct tcaaaagttg gagttataaa
     3421 agcagttaga gaaataacag gattaggatt aaaagaagct aaagaagtag ttgacaatgc
     3481 tcctaagaca gttaaagaag gagcttctaa agaagaagct gatcaaataa aagagaaatt
     3541 agaagctgct ggagctaaag tagaagttaa gtagtttctt taaaaagata cccattgggt
     3601 atcttttttt atttataaaa aactataaag aaagtatttg gaattataaa gaaagtgttt
     3661 ttataaaata aaaaaaattt atataatagt actaaattaa gggtatcata tatggtgtaa
     3721 tggtatttta atttaacatt attatcaaat taaaatataa acaattgttg taatgtctgt
     3781 atataaagta tataattaaa ctaaaatgta aaattttaca ttttagttta aaaaaataca
     3841 cgagtgtttt ataaataaaa tgtaataaat aattaattaa caaatataaa ttttaaaata
     3901 agttagatat ttagaaaaat cgaaatttgt aggatttaaa ggacatattc aaggaggagt
     3961 taataatgga aaaattacaa ggaaaaattg cagtagttac tgcagcaaca aaaggtattg
     4021 gattagcatc agcagagata ttagcaaaaa atggagcaac tgtgtactta gcagctcgtt
     4081 cagaagaatt agctaatgaa gttataaata aaataagtgc agaaggtggt tgtgctaagt
     4141 ttgtttactt taatgctcgt gaagaagaga cctttacctc aatgatagaa gaggtagtta
     4201 aaaaagaagg taagatagat atacttgtaa acaattttgg ttcaacaaac ccttctcttg
     4261 acaaagacct tgtaactggg gatacagata atttctttga cacagtaaat actaatttaa
     4321 aaagtgtgta tttaccatgt aaagcagcaa ttcctcatat gataaagaat ggaaaaggaa
     4381 gtatagtaaa tatatcaagt ataggttcag tattacctga tttatctaga atagcttact
     4441 gtgtatcaaa agcagcaatc aactcattaa ctcaaaacat agctacacaa tatgctaaag
     4501 acaatgttag atgtaatgct gtacttccag ggcttattgc aactaaagca gcattagaca
     4561 atatgtctcc agagttcata aaagaatttt taaagcatgt tcctttaaat cgtatagggg
     4621 aaccagatga tatagcaaaa gctgttctat tctatgctag tgatgactca tcatttataa
     4681 caggggattt acttgaagtt gcaggaggat ttggtttacc tactccacaa tttgcagata
     4741 atatattagg ataattaaat tactaatatg caatgaaagc ttatgcatta gttatagtag
     4801 aaatgatatt tttataaagg ccatagatat aatttatatt ggaaaggttt gaggttcaaa
     4861 attccaagtt aaatttattt atggtcttta ttttgtatat tttaataaat tgtatatatt
     4921 tgtataaatg taattattta caatcatttt aatatggaat tataattaaa taggggggat
     4981 ggtacttgta taaaattttt tatagaaaat tagacctata aatgaaaaat atggatttta
     5041 tattggaaaa ttgtacataa tacataatat atgcttaaaa taatttacaa aaaatcaaaa
     5101 ataatgcata gtttacatat aagataaaaa atatattgaa aaatacttga cattatgcta
     5161 aaattattag atgcgttaga atgaactatt gttttatttt taattattga gaggtgaaaa
     5221 gtgaatgcca catcctgtca cgataggtaa aagaactaga atgagctttt ctaaaattaa
     5281 ggaaatagct gatgtgccaa atcttataga aattcaagta gattcctatg agtggttttt
     5341 aaaagaaggt ttaaaagaag tgtttgatga tatttcacca atagaagatt atactggtaa
     5401 tcttatattg gaatttgtcg actattctct agatgataaa ccaaagtacg atatagaaga
     5461 gtgtaaagag agagatgcta catattgtgc acctctaaag gttaaggtaa gacttataaa
     5521 taaagaaaca ggtgagataa aagaacaaga agtgtttatg ggtgacttcc ctttaatgac
     5581 agaaagagga actttcgtta taaatggagc agaaagagtt atagtaagtc aattagttag
     5641 atctcctggt gtttactatg cggaagaaag agataaaaca ggtaagagat tgatttcatc
     5701 tactgtaatt cctaatagag gagcatggtt agaatatgaa acagattcta atgatgtaat
     5761 atctgtaaga gttgatagaa caagaaaaca accagttaca gttttactta gagctttagg
     5821 aataggaaca gatgcagaga taattgacct tttaggagaa gatgagagat tatctgctac
     5881 attagaaaaa gataatacta agacagtaga agaaggtctt gtagagatat acaaaaaatt
     5941 aagaccaggt gaacctccta ctgtagaaag tgcatcatct ttattaaatg ctttattctt
     6001 tgacccaaaa agatatgacc tagcaaaagt tggtagatat aaattcaata agaaacttgc
     6061 tttatgttat agaataatga ataaaatttc agcagaagac ataataaatc cagaaactgg
     6121 agaagtattt gtaaaagctg gagaaaaaat aagctatgat ttggctaaag ccatacaaaa
     6181 tgcaggtata aatgtagtaa atctactaat ggatgatgat aaaaaagtta gagttatagg
     6241 aaataatttt gtggatatta agtctcacat tgattttgac atagatgatt taaacataaa
     6301 agaaaaagta cattatccaa ctttaaaaga aattttagat ggatatagtg atgaagaaga
     6361 aataaaagag gctataaaat caagaattaa agaacttata ccaaaacata tattattaga
     6421 tgatataata gcttcaataa gttatgagtt caatatattc tataatatag gaaatattga
     6481 tgatatagat cacctaggaa acagaagaat aagatctgta ggtgaattgt tacaaaatca
     6541 agttagaatt ggtctttcaa gaatggaaag agttataaaa gaaagaatga cggttcaaga
     6601 tatggaagct ataacgcctc aagcgttagt taacataaga ccagtttcag ctgcaataaa
     6661 agaatttttt ggtagttctc agttatctca gttcatggat caaacaaatc ctctatctga
     6721 gttaacgcat aagagaagat tatcagccct tgggcctgga ggtctttcaa gagaaagagc
     6781 tggattcgaa gtgcgtgacg ttcaccattc acactatggt agaatgtgtc caatagagac
     6841 tccagaggga ccaaatatcg gtcttataaa ctctctagga acttatgcaa aaataaatga
     6901 atttggattt atagaatcac catatagaaa atttgataaa gaaacatcaa cagttactga
     6961 tgaaatacat tatttaactg ctgacgaaga agatttattc gtaagagctc aggcaaatga
     7021 gccattaaca gaagatggta aattcgtaaa ccatagagtt gtttgtagaa ctgtaaatgg
     7081 tgctgttgaa atggttcctg aaagtagagt agattacatg gatatatctc ctaagcaggt
     7141 tgtatctgtt gccactgcta tgataccttt cttagaaaat gatgatgcca accgtgccct
     7201 tatgggagcg aacatgcagc gtcaggcagt gcctctagtt agaagagaag caccaattat
     7261 aggaactggt atagaataca gagcagcaaa agactctgga gcagttgttg ttgctagaaa
     7321 ttctggtata gcagaaagag taacagcaga tgaaataatt ataaaaagag aagatggaaa
     7381 cagagataga tataatctac ttaagtttaa acgttcaaac tcaggtactt gtataaatca
     7441 aacacctata ataaataagg gtgaccagat aataaaaggt gatgttatag cagatggtcc
     7501 agcaactgat ttaggagaag ttgcacttgg aagaaactgt cttatagcat tcatgacttg
     7561 ggaaggttat aactacgagg atgctatatt aataaatgaa agattagtta aagaagatag
     7621 attatcaaca attcatatag aagaatatga atgtgaagct agagatacaa aactagggcc
     7681 agaggaaata actagagata tacctaatgt tggagatagt gcaatcaaaa acttagatga
     7741 tagaggtata ataagaatag gtgcagaagt agactcaggt gatatattag ttggtaaggt
     7801 aactccaaaa ggagaaactg aacttactgc tgaggaaaga ttacttcgtg cgatattcgg
     7861 agaaaaagcc agggaagtta gagatacttc acttaaagta cctcatggtg aatctggtat
     7921 aatagttgat gtaaaagtat ttactagaga aaatggagat gatttatctc caggagtaaa
     7981 tgaattagta agatgttata tagctaaaaa gagaaagata aaagttggag ataagatggc
     8041 tggtcgtcat ggtaataaag gggttatctc gagagtatta cctgaagaag atatgccatt
     8101 catggaaaat ggaacgccat tagatataat acttaaccca caaggtatac catcacgtat
     8161 gaacatcggt caggttctag aagttcattt aggacttgct gctaagacat taggatggta
     8221 tgtagcaaca tcagtattcg atggtgctaa tgagtatgac ataatggatg ctcttgaaga
     8281 agctggatat cctagagatg gtaaattaac tttatatgat ggtagaacag gtcaatcttt
     8341 tgacaataga ataactgttg gatatatgta ttacttgaaa ctacatcact tagtagatga
     8401 aaaactacat gcaagaagta ctggaccata ctcattagtt actcaacagc cactaggtgg
     8461 taaagcacaa tttggtggac aaagatttgg agagatggag gtttgggctc tagaagcata
     8521 tggagcggct cacatacttc aagagatact tactgttaag tctgatgatg ttgtaggtcg
     8581 tgttagaact tatgaagcta tagttaaagg tgaaaacata cctgagccag gaatcccaga
     8641 atcatttaag gttcttataa aggaacttca aagtttatgc ttagatgtaa aagtattgac
     8701 agatgaagac caagaaatag aagtaagaga atctgtagat gaagatgata cgataggtga
     8761 atttgagcta gacgttgtaa atcacatggg agaagttgaa gaaagtaata taatagaaga
     8821 aattgaagat gattttgtgg aagccacaga agatgaggat atagagaatt tagaagaatt
     8881 cactgaagat gatttatttg aagaagaaat agattttgaa agtgatgact ttgatatgta
     8941 aatttaatta atgtaaatct caaatagaag ggagagaact ccttgtttga attaaacaat
     9001 ttcgagtcga taaaaatagc attggcttct ccagaaaaaa taagacaatg gtctagagga
     9061 gaagttaaaa agccagaaac tataaattac cgtactttaa agccagaaaa agatggtctt
     9121 ttctgtgaaa ggatatttgg accacaaaaa gactgggagt gtcattgtgg taaatataga
     9181 agagttagat ataaaggtgt agtttgtgat agatgtggag tagaagtaac taaatcaaaa
     9241 gtaagaagag agagaatggg acatatagag ctagctgctc ctatgtctca catctggtac
     9301 ttcaaaggta taccaagtag aatgggactt ttacttgata tgtcaccaag atcgttagaa
     9361 aaaatactat atttcgcctc atatgtagta gttgatccag gtgaaactgg attgaatgaa
     9421 aaacagttac ttacagaaaa agaatacaga actgctcttg aaaagtatgg atatactttt
     9481 actgtgggta tgggtgctga agctgtaaaa acattactac aaaatataga tttagaacaa
     9541 caaagtaaag acttaagagc ggaattaaaa gatagtacag gacaaaagaa agtaagaaca
     9601 attagaagat tagaagttgt agaagcattt aaaaaatctg gaaataaacc agaatggatg
     9661 attttagatg caataccagt aataccgcca gatttaagac caatggtaca acttgatggt
     9721 ggaagatttg caacttcaga cctaaatgat ttatacagaa gagttataaa tagaaataat
     9781 agacttaaaa gactattaga gcttggagct ccagatataa ttgtaagaaa tgaaaaaaga
     9841 atgcttcaag aagctgttga tgcattgata gataatggta gaagaggtag acctgtaaca
     9901 ggacctggaa atagaccact taaatcttta tcagatatgt taaaaggtaa gcaaggtcgt
     9961 ttccgtcaaa acttacttgg taagcgtgtt gactactcag gacgttctgt tatagttgtt
    10021 ggaccagaac ttaaatttta tcaatgtgga cttccaaaga agatggcatt agaattattc
    10081 aagccatttg ttatggataa attagttaaa gaaggttatg cacataatat aaaaagtgcg
    10141 aaatctatag tagaaaaagt taagccagaa gtttgggatg ttttagaaga tgttataaaa
    10201 agtcatccag ttcttcttaa ccgtgcaccg actctacata gattaggtat acaagcattt
    10261 gaaccaatct tagttgaagg taaggctata aaactacatc ctcttgtatg tacagcttat
    10321 aatgcagact ttgatggtga ccaaatggca gtccatgtac ctttatcagt agaagcacaa
    10381 gcagaagcaa gattcttaat gctttctgta aataatatac ttgctcctaa agatggttca
    10441 cctataacta ctccatctca ggatatggtt ttaggttgct attatctaac aatagaagcc
    10501 caagatggag ctaaaggaac aggtatggta tttaaagact ttaatgaact attacttgct
    10561 tattacaata aatcagttca tctacatgca ttagtgaaac tgaaggtaac acttgaagat
    10621 ggaagaagtt cattggttga aagtactgtt ggtagattta tatttaatga aaatatacct
    10681 caagacttag gttttgtaga tagaaaagaa aatccatttg cacttgaagt tgatttctta
    10741 gcagataaaa aatctcttgg taaaataata gataaatgct ttagaaaaca cggaaataca
    10801 gaaacagctg aattactaga ttatataaaa gctctaggat ttaaatattc tacattaggt
    10861 ggtataacag ttgctgttga tgatatgagt gtcccagaag aaaagaaagt atttatagct
    10921 gaggcagaag ctaaagttga taagtatgaa aaagcataca gaagaggtct aatctctgat
    10981 gaagaaagat atgaaaaagt tatagagaca tggacagaaa caactgataa agttactgat
    11041 gctcttatgg gtggactaga tagattaaat aatatatata taatggcaca ttcaggagcc
    11101 agaggttcta aaaaccaaat tagacagctg gcaggtatgc gtggtcttat ggcaaatgca
    11161 tctggtaaaa cagtcgagat accagttaaa tctaatttcc gtgaaggttt atcagtacta
    11221 gaatacttta catcttcaca tggtgccaga aaaggtcttg ccgatacagc tatacgtaca
    11281 gctgaatctg gatatttaac aagaagactt gttgatgtaa gtcaagatgt tattgtaagg
    11341 gaaatagatt gtggaacaga agatactaca gagatttatg ctataaaaga aggaaatgaa
    11401 gttatagaag agatatatga tagaattgta ggaagatata ctatagaccc tatattaaat
    11461 cctgaaactg gagaagttct agttgaggct gattcaatga tacaagaaga tgaagcagaa
    11521 actatagtag ctttagggat tgaaaaaatt aggataagaa cagttcttaa ctgtaaaact
    11581 aatcatggag tttgttctaa gtgttatggt agaaacttgg caacaggaaa agaagttaat
    11641 ataggtgaag cagttggtat aatagcagct caatctatcg gtgagccggg tactcagctt
    11701 acaatgcgta cattccatac tggaggagtt gcaggagctg atataactca aggtctacca
    11761 agggttgaag aattatttga agctagaaaa ccaaaaggat tagctgtaat aactgaagta
    11821 tctggtagag ttgaaattga tgaaacagga aagagaaaag aagtaaatgt aataccagaa
    11881 gaaggcgaga ctcaaacata tgtaattcca tatggttcta gattaaaagt taagcaaggt
    11941 caaatgctag aggctggaga ccctctaaca caaggattta taaatcctca tgatatagta
    12001 agagtaaatg gtgttaaggg agttcaagaa tatatagtta aagaagttca aagagtgtat
    12061 agacttcaag gggttgatgt taatgataag catatagaag ttatagtaag acaaatgcta
    12121 tctaaagtaa aagttgaaga cccaggagat acagatttat tgccaggtgg atatgaagat
    12181 gtattaacat tcaatgaatg taataaagat gctatagata aaggtctaag accagcagtt
    12241 gctaaaagag ttttacttgg tataactaaa gcatctcttg caactgattc attcttatca
    12301 gcagcttctt tccaagaaac aacaagagta ttaacagagg cagcaataaa aggtaaagaa
    12361 gaccacttaa taggacttaa agaaaatgtt atattaggta agttaatacc agcaggaaca
    12421 ggaatgaaga aatatagaaa tatagctgtt gaaaaaattg aagattaata gcatatagag
    12481 taaagaaatc ttgacaacca ttatttgtgg tgttaaaata gtaaagtgtg ttgtttaaaa
    12541 gtacactaag ggcttgaaca agttcttagt gacttttaga tggcaataaa tgagaactaa
    12601 aaagcatcaa gctagaaaag tgctaatatc acatatttat gtgttataga cattaaattg
    12661 taagtaacga atgttacaat cagtcagtat gaatattcat attgatgtag ttggcggtca
    12721 ctgataagat cgaaaatttg gaaggaggtg cagatgatgc caacaattaa ccaattagtt
    12781 cgtaaaagta gaaaagcgct agagaagaaa tctactgctc cagcattaca aaaaggttac
    12841 aactctttaa ataagaaagt aactgacgct agtgccccac aaaaaagagg ggtttgtact
    12901 tcagtaaaaa cagttactcc taagaagcct aactcagctt taagaaaagt tgccagagtt
    12961 agattaacta atggtataga agtttctgct tatataccag gagaaggaca caacttacaa
    13021 gagcatagtg ttgttcttat aagaggagga agagtaaaag accttccagg ggttagatac
    13081 catatactaa gaggtactct agatacagca ggtgttgata agagaagaca atcaagatct
    13141 aaatacggtg cgaagagacc taaagaagct aaaaaataaa acaattaatg attgattata
    13201 gttcggtgct ttaaatatat atatatttac ggcatcacgg cattaaatga tttatgtcga
    13261 gtacctatga tttaagttaa ataattaagg agggaagcga aatgccaaga aaaggtaata
    13321 ttccaaaaag agaagtttta ccagatccta tgtatgggag taaggtagtt actaaattaa
    13381 taaataatct aatggtagac ggaaaaaaag gaaagtctca aagaatagtt tatgatgctt
    13441 tcgcaattgt tgctgaaaag acaggagaag aagctttaga agtatttaat aaagcaatgg
    13501 ataatataat gcctgtttta gaagtaaaag caagaagagt tggtggggca aattaccaag
    13561 tgccaatcga agttagacct gaaagaagac aaacattagg tttaagatgg ctagtaaaat
    13621 atactagagc tcgtggagaa aaaggaatgg ttgaaaagct agctaaagag ataatggacg
    13681 cagctaacaa cactggagct tcagttaaga aaaaagaaga tactcataaa atggcagaag
    13741 ctaataaagc atttgctcat tacagatggt aggatatata gatatcttat aaattcaagg
    13801 atatcataag gaggataacc atggctagaa agtttccttt ggaaaaaact aggaatatag
    13861 gaatcatggc tcatatagat gcaggaaaga ctactactac agaaagaatc ctgttttata
    13921 ctgggcaaac tcataaaata ggagaaactc atgaaggagc ttctcaaatg gactggatgg
    13981 agcaagaaaa ggaaagaggt ataacaataa cttctgccgc tactactgct tcatggaaag
    14041 accatagaat aaacataata gatactcctg gacacgtgga cttcacagtt gaggttgaaa
    14101 gatctctaag agttcttgat ggttcagttg ctgtattctg tgcaaaaggt ggggttgagc
    14161 ctcaatctga aaatgtatgg agacaagctg aaacttatgg agtacctaga atagctttta
    14221 taaacaaaat ggatatatta ggtgcagatt tctacaatgt tataagcatg atgaaatcta
    14281 gattaaattc taatgcagtt cctatgcaat taccaatagg taaagaagat tcatttatag
    14341 gtataataga cttattaaaa atggatgctg ttatatacaa agacgaccta ggggttgaaa
    14401 tggaagaaac agatatacca gaagatatga aagaattagc tgctgagtgg agagaaaaat
    14461 tagttgaatc agtagctgaa actgatgaag aattaatgat gaaatacctt gaaggtgaag
    14521 aacttactat agatgaatta aaagttgcta taagaaaagc tactatagct tgtgaaatga
    14581 acccagtttt ctgtggtaca gcttatagaa acaaaggtgt tcagttagta atagatgcag
    14641 ttttagatta cttaccagca ccaactgata taccagctat aaaaggtata ttagcagatg
    14701 gagaagaagc tgaaagacat tcttctgatg aagagccatt ctctgcatta gcattcaaaa
    14761 taatgactga cccattcgtt ggaaaactag cgttcttcag agtttattct ggaacattag
    14821 aatcaggttc ttatgtttta aatgcaacta agaataagag agaaagaatt ggtcgtatat
    14881 tacaaatgca tgctaataca agagaagaaa taactaaagt gtatgctgga gatatagcag
    14941 cagcagtagg attaaaagat actactactg gagacacatt atgtgaccct gctaatccaa
    15001 taatacttga atctatggaa ttccctgagc cagttatatc tgttgctata gaaccaagtt
    15061 ctaaagcagc tcaagaaaaa atgggtatag ctcttcaaaa attagctgaa gaagacccaa
    15121 ctttcacagt taagactgac caagaaacag gacaaactat aatatctggt atgggtgaat
    15181 tacacttaga gataatagta gatagattat taagagaatt taaagtagaa gctaaagttg
    15241 gtgcaccaca agttgcatat agagagacta taactcaacc agttgatgtt gaatacaagt
    15301 actctaagca atcaggtggt agaggacaat acggtcacgt taagattaga gttgctcctc
    15361 aagagccagg tgaaggatac aagttcacaa acaaaactgt tggtggatca gtaccaaaag
    15421 aatatgttgg tccagttgac atgggtatac aaggagctat gcaatctggt atagttgctg
    15481 gatatccagt tgtagacgtt gctgttgagt tatatgatgg ttcttaccat gaggttgact
    15541 cttctgagat ggcgtttaag atggctggtt caatggcatt caaagatgct atgaagaaag
    15601 gtaatgcagt tcttttagag ccttacttca aagtagaagt tgttactcca gaagattaca
    15661 tgggagatgt tatgggtgac ttaaactcaa gaagaggatt aatccaaggt atggaagcta
    15721 gaagtggtgc acaagttata aatgcattcg ttccactttc agaaatgttt ggatactcaa
    15781 cagacctaag atcttcaact caaggtcgtg caacttacac aatgatattc gatcactatg
    15841 agcaagttcc agcttcagtt gctaagaaaa tagctgaagg aaaataattt aaaaataatt
    15901 aataatataa atataaaaaa taatcagcta gatgagatat atctcatcta gcacaagat
//
LOCUS       DAELSY010000090        15940 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.090, whole genome shotgun sequence.
ACCESSION   DAELSY010000090 DAELSY010000000
VERSION     DAELSY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15940
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.090"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            105..659
                     /gene="tcdR"
                     /locus_tag="KPG09_002502"
     CDS             105..659
                     /gene="tcdR"
                     /locus_tag="KPG09_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBG0967103.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSVFEEDICFFEYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            990..8090
                     /gene="tcdB"
                     /locus_tag="KPG09_002503"
                     /gene_synonym="toxB"
     CDS             990..8090
                     /gene="tcdB"
                     /locus_tag="KPG09_002503"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBG0967104.1"
                     /translation="MSLVNRKQLEKMANVRFRVQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTIVESATNDTLESFREN
                     LNDPRFDYNKFYRKRMEIIYDKQKNFINYYKTQREENPDLIIDDIVKIYLSNEYSKDI
                     DELNSYIEESLNKVTENSGNDVRNFEEFKGGESFKLYEQELVERWNLAAASDILRISA
                     LKEVGGVYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMVKLEAIMKYKEYIPGYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNAFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKKNYFEGSLGEDDNLDFSQNTVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGLDVDSLSTEIETAIDLA
                     KEDISPKSIEINLLGCNMFSYSVNVEETYPGKLLLRVKDKVSELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKIIVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLAECEINVISNIDTQVVEGRIEEAKSLTSDSINYIK
                     NEFKLIESISDALYDLKQQNELEESHFISFEDILETDEGFSIRFIDKETGESIFVETE
                     KAIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDATHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     VIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTAATTAIITSSLGIASG
                     FSILLVPLAGISAGIPSLVNNELILRDKATKVVDYFSHISLAESEGAFTSLDDKIMMP
                     QDDLVISEIDFNNNSITLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPVITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMNINIELNENDTWVIDVDNVVRDVTIESDKIKKGDLIENILSKLSIE
                     DNKIILDNHEINFSGTLNGGNGFVSLTFSILEGINAVIEVDLLSKSYKVLISGELKTL
                     MANSNSVQQKIDYIGLNSELQKNIPYSFMDDKGKENGFINCSTKEGLFVSELSDVVLI
                     SKVYMDNSKPLFGYCSNDLKDVKVITKDDVIILTGYYLKDDIKISLSFTIQDENTIKL
                     NGVYLDENGVAEILKFMNKKGSTNTSDSLMSFLESMNIKSIFINSLQSNTKLILDTNF
                     IISGTTSIGQFEFICDKDNNIQPYFIKFNTLETKYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVIISPNIYTDEINITPIYEANNTYPEVIVLDTNYIS
                     EKINININDLSIRYVWSNDGSDFILMSTDEENKVSQVKIRFTNVFKGNTISDKISFNF
                     SDKQDVSINKVISTFTPSYYVEGLLNYDLGLISLYNEKFYINNFGMMVSGLVYINDSL
                     YYFKPPIKNLITGFTTIGDDKYYFNPDNGGAASVGETIIDGKNYYFSQNGVLQTGVFS
                     TEDGFKYFAPADTLDENLEGEAIDFTGKLTIDENVYYFGDNYRAAIEWQTLDDEVYYF
                     STDTGRAFKGLNQIGDDKFYFNSDGIMQKGFVNINDKTFYFDDSGVMKSGYTEIDGKY
                     FYFAENGEMQIGVFNTADGFKYFAHHDEDLGNEEGEALSYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGISIINDGKYYFNDSGIMQIGFVTINNEVFYFS
                     DSGIVESGMQNIDDNYFYIDENGLVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFDPETKKAYKGINVIDDIKYYFDEN
                     GIMRTGLITFEDNHYYFNEDGIMQYGYLNIEDKTFYFSEDGIMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8212..8712
                     /gene="tcdE"
                     /locus_tag="KPG09_002504"
     CDS             8212..8712
                     /gene="tcdE"
                     /locus_tag="KPG09_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBG0967105.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAVLLDAMTDEM
                     NAKDEK"
     gene            8791..8952
                     /locus_tag="KPG09_002505"
     CDS             8791..8952
                     /locus_tag="KPG09_002505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967106.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYMDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            9041..9223
                     /locus_tag="KPG09_002506"
     CDS             9041..9223
                     /locus_tag="KPG09_002506"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967107.1"
                     /translation="MRDEGQNDRENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            9440..>15940
                     /gene="tcdA"
                     /locus_tag="KPG09_002507"
                     /gene_synonym="toxA"
     CDS             9440..>15940
                     /gene="tcdA"
                     /locus_tag="KPG09_002507"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBG0967108.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKNSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     LLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTIPRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKDSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKEYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPYISSHIPSLSV
                     YSAIGIKTENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENNGTKLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNTKIKLDKDTRNFIMPTITTDEIRNKLSYSFDGA
                     GGTYSLLLSSYPISMNINLSKDDLWIFNIDNEVREISIENGTIKKGNLIEDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNGSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLNNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIESNLVTGWQTINGKKYYFDINTGAASTSYKIINGKHFYFNNNGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSGSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTSIASTGYTIINGKYFYFNTDGIMQIGVFKVP"
BASE COUNT         6433 a         1697 c         2509 g         5301 t
ORIGIN      
        1 tttaatttat ttgccgatta tataattata atgactgatt taattccaat gttgtcaaaa
       61 tttttaaata aatcatcatt ataaatataa gagaggatga ttttatgcaa aagtcttttt
      121 atgaattaat tgttttagca agaaataact cagtagatga tttgcaagaa attttattta
      181 tgtttaagcc attagtaaaa aaacttagta gagttttaca ttatgaagag ggagaaacag
      241 atttaataat attttttatt gaattaataa aaaatattaa attaagtagc ttttcagaaa
      301 aaagcgatgc tattatagtc aaatatattc ataaatcatt actgaataag acttttgagt
      361 tgtctagaag atattctaaa atgaagttta attttgtaga atttgatgaa aatatcttaa
      421 atatgaaaaa taattatcaa agtaagtctg tttttgagga agatatttgt tttttcgaat
      481 atattttgaa agaattatct ggtattcaaa gaaaagttat tttttataaa tatttaaaag
      541 gatattctga tagagaaata tcagtgaaat taaaaatatc tagacaagct gttaataagg
      601 ctaaaaatag agcatttaaa aaaataaaaa aagactatga aaattatttt aacttgtaat
      661 taatgaattt aaagaaatat ttacaataga aatcaaattt tagaattaac tttattgtaa
      721 aatcaataac ttaatctaag aatatcttaa tttttatatt ttatatagaa caaagtttac
      781 atatttattt cagacaacgt ctttattcaa tcgaagagca aattaatcaa ctgagtgtct
      841 tcaatttaaa atgttaggaa gtgaatgtat atgaaaacct aagtagatat tagtatattt
      901 tataaataga aaggaggata tataaaagag ttttagcatt tagatgtaaa aatattcaat
      961 aaaaatatta tagtaaagga gaaaatttta tgagtttagt taatagaaaa cagttagaaa
     1021 aaatggcaaa tgtaagattt cgtgttcagg aagatgaata tgtagcaata ttggatgctt
     1081 tagaagaata tcataatatg tcagagaata ctgtagtcga aaaatattta aaattaaaag
     1141 atataaatag tttaacagat atttatatag atacatataa aaagtctggt agaaataaag
     1201 ccttaaaaaa atttaaggaa tacctagtta cagaagtatt agagctaaag aataataatt
     1261 taactccagt tgagaaaaat ttacattttg tttggattgg aggtcaaata aatgacactg
     1321 ctattaatta tataaatcaa tggaaagatg taaatagtga ttataatgtt aatgtttttt
     1381 atgatagtaa tgcatttttg ataaacacat tgaaaaaaac tatagtagaa tcagcaacaa
     1441 atgatacact tgaatcattt agagaaaact taaatgaccc tagatttgac tataataaat
     1501 tttacagaaa acgtatggag ataatctatg ataagcagaa aaatttcata aattactata
     1561 aaactcaaag agaagaaaat cctgacctta taattgatga tattgtaaag atatatcttt
     1621 caaacgagta ttcaaaggat atagatgaac ttaattccta tattgaagag tcattaaata
     1681 aagttacaga aaatagtggg aatgatgtta gaaactttga agaatttaaa ggtggagagt
     1741 cattcaaatt atatgaacaa gagttggtag aaagatggaa tttggcagct gcttctgaca
     1801 tattaagaat atctgcctta aaagaagttg gtggtgtata tttagatgtt gatatgttac
     1861 caggaataca accagaccta tttgagtcta tagagaaacc tagttcagta acagtggatt
     1921 tttgggaaat ggtaaaatta gaagctataa tgaaatacaa agaatacata ccaggatata
     1981 cttcagagca ttttgatatg ttggatgaag aagttcaaag tagttttgaa tctgttctag
     2041 cttctaagtc agataagtca gaaatattct catcacttgg cgatatggag gcatcaccac
     2101 tagaagttaa aattgcattt aatagtaaag gtattataaa tcaaggacta atttctgtga
     2161 aagactcata ttgtagcaat ttaatagtaa aacaaatcga gaacagatat aagatattga
     2221 ataatagttt aaatccagct attagcgagg ataacgattt caatactaca acgaatgctt
     2281 ttattgatag tataatggct gaagctaatg cagataatgg tagatttatg atggaactag
     2341 gaaagtattt aagagttggt ttcttcccag atgttaaaac tactattaac ttaagtggcc
     2401 ctgaagcata tgcggcagct tatcaagatt tattaatgtt taaagaaggt agtatgaata
     2461 tccatttgat agaagctgac ttaagaaact ttgaaatctc taaaactaat atttctcaat
     2521 caactgaaca agaaatggct agcttatggt catttgacga tgcaagagct aaagctcaat
     2581 ttgaagaata caaaaaaaat tactttgaag gttctcttgg agaagatgac aatcttgact
     2641 tttctcaaaa tacagtagtt gacaaggagt atcttttaga aaaaatatct tcattagcaa
     2701 gaagttcaga gagaggatat atacactata ttgttcagtt acaaggagat aaaattagtt
     2761 atgaagcagc atgtaactta tttgcaaaga ctccttatga tagtgtactg tttcagaaaa
     2821 atatagaaga ttcagaaatt gcatattatt ataatcctgg agatggtgaa atacaagaaa
     2881 tagacaagta taaaattcca agtataattt ctgatagacc taagattaaa ttaacattta
     2941 ttggtcatgg taaagatgaa tttaatactg atatatttgc aggtcttgat gtagattcat
     3001 tatccacaga aatagaaaca gcaatagatt tagctaaaga ggatatttct cctaagtcaa
     3061 tagaaataaa cttactggga tgtaacatgt ttagctattc tgtaaatgta gaggagactt
     3121 atcctgggaa attattactt agagttaaag ataaagtatc agaattaatg ccatctataa
     3181 gtcaagactc tattatagta agtgcaaatc aatatgaagt tagaataaat agtgaaggaa
     3241 gaagagaatt attggatcat tctggtgaat ggataaataa agaagaaagt attataaagg
     3301 atatttcatc aaaagaatat atatcattta atcctaaaga aaataaaatt atagtaaaat
     3361 ctaaaaattt acctgaatta tctacattat tacaagaaat tagaaacaat tctaattcaa
     3421 gtgatattga actagaagaa aaagtaatgt tagcagaatg tgagataaat gttatttcaa
     3481 atatagatac acaagtggta gaaggaagga ttgaagaagc taaaagctta acttctgact
     3541 ctattaatta tataaagaat gaatttaaac taatagaatc tatttctgat gcactatacg
     3601 atttaaaaca acagaatgaa ttagaagagt ctcattttat atcttttgag gatatattgg
     3661 agactgatga aggctttagt ataagattta ttgataaaga aactggagaa tctatatttg
     3721 tagaaactga aaaggcaata ttctctgaat atgctaatca tataactgaa gagatttcta
     3781 agataaaagg tactatattt gatactgtaa atggtaagtt agtaaaaaaa gtaaatttag
     3841 atgctacaca tgaagtgaat actttaaatg ctgcattttt tatacaatca ttaatagaat
     3901 ataatagttc taaagaatct cttagtaatt taagtgtagc aatgaaagtt caagtttatg
     3961 ctcaattatt tagtactggt ttaaatacta ttacagatgc agccaaagtt gttgaattag
     4021 tatcaactgc attagatgaa actatagatt tacttcctac attatctgaa ggattacctg
     4081 taattgcaac tatcatagat ggtgtaagtt taggtgcagc aatcaaagag ctaagtgaaa
     4141 caagtgaccc attattaaga caagaaatag aagctaagat aggtataatg gcagtaaatt
     4201 taacagcagc tacaactgca atcattactt catctttagg aatagctagt ggatttagta
     4261 tacttttagt tcctttagca ggaatttcag caggtatacc aagtttagta aacaatgagc
     4321 ttatcctaag agataaagca acaaaagttg tagattattt tagtcatata tcattagctg
     4381 agtctgaagg agcatttact tcattagatg ataaaataat gatgccacaa gatgatttag
     4441 taatatctga gatagacttt aataacaatt caataacttt aggtaaatgt gaaatctgga
     4501 gaatggaagg tggctcaggt catactgtaa ctgatgatat agatcacttc ttttcagcac
     4561 catcaataac atatagagag ccacacttat ctatatatga cgtattggaa gtacaaaaag
     4621 aagaacttga tttgtcaaaa gatttaatgg tattacctaa tgctccaaat agagtatttg
     4681 cttgggaaac aggatggaca ccaggtttaa gaagcttaga aaatgatggc acaaaactgt
     4741 tagaccgtat aagagataac tatgaaggtg agttttattg gagatatttt gcttttatag
     4801 ctgatgcttt aataacaaca ttaaaaccaa gatatgaaga tactaatata agaataaatt
     4861 tagatagtaa tactagaagc tttatagtcc cagtgataac tacagaatat ataagagaga
     4921 aattatcata ttctttttat ggttctggag gaacttatgc attatctctt tctcaataca
     4981 atatgaatat aaacatagaa ttaaatgaaa atgatacttg ggttatagat gtcgacaatg
     5041 tcgtaagaga tgtcactata gaatctgata aaattaaaaa aggagattta atagagaata
     5101 ttttatctaa attaagtatt gaagacaata aaattatttt agataatcat gaaattaatt
     5161 tctctggaac attaaatgga ggtaatggat ttgtatcttt aacattctca atcttagaag
     5221 gaataaatgc agttatagaa gttgatttat tatctaaatc atataaagtt cttatttctg
     5281 gtgaactaaa aacattgatg gcaaattcaa attctgttca acagaaaata gattatatag
     5341 gattgaacag cgaattacaa aaaaatatac cttatagttt tatggatgat aaaggaaaag
     5401 aaaatggatt tattaattgt tctacaaaag aaggtttatt tgtatctgaa ttatctgatg
     5461 tagttcttat aagtaaagtt tatatggaca atagtaaacc tctatttgga tattgtagta
     5521 atgatttgaa agatgttaaa gtcataacta aagatgacgt tattatatta acaggatatt
     5581 atttaaaaga tgatataaaa atctctcttt cttttactat acaagatgaa aatactataa
     5641 aattaaatgg agtatattta gatgaaaatg gagtagctga aatattgaaa tttatgaata
     5701 aaaaaggtag tacaaatact tcagattctt taatgagctt tttagaaagt atgaatataa
     5761 aaagtatttt cataaattcc ttacaatcta atactaagct tatattagat actaatttta
     5821 taataagtgg tactacttct attggtcaat ttgagtttat ttgtgataaa gataataata
     5881 tacaaccata tttcattaag tttaatacac tagaaactaa atatactcta tatgtaggta
     5941 atagacaaaa tatgatagta gaaccaaatt atgatttaga tgattctgga gatatatctt
     6001 caactgtcat taatttttct cagaaatacc tttatggaat agacagttgt gttaataaag
     6061 ttataatttc gccaaatata tatacagatg aaataaacat aacacctata tatgaagcaa
     6121 ataatactta tccagaagtg attgtattag atacaaatta tataagtgaa aaaatcaata
     6181 ttaatatcaa tgatttatct atacgatatg tatggagtaa tgatggaagt gattttattc
     6241 ttatgtcaac tgatgaagag aacaaggtat cacaagttaa aataagattt actaatgttt
     6301 ttaaaggtaa tactatatca gataagatat cttttaattt tagtgataag caagatgtat
     6361 ctataaataa agttatttca acatttacac cttcatatta tgtggaagga ttacttaatt
     6421 atgatttagg tctgatttct ttatacaatg agaaatttta tattaataac tttggaatga
     6481 tggtgtctgg attagtatat attaatgatt cattatatta tttcaagcca ccaataaaga
     6541 acttgataac tggatttaca actataggtg atgataaata ctactttaat ccagataatg
     6601 gtggagctgc ttcagtcgga gaaacaataa ttgatggcaa aaactactac ttcagccaaa
     6661 atggagtgtt acaaacaggt gtatttagta cagaagatgg atttaaatat tttgctccag
     6721 cagatacact tgatgaaaat ctagaggggg aagcaattga ttttactggc aaactaacta
     6781 ttgatgaaaa tgtttattat tttggagata attatagagc agctatagaa tggcaaacat
     6841 tagatgatga agtgtactat tttagtacag atacaggtag agcttttaaa gggctaaatc
     6901 aaataggtga tgataaattc tatttcaact ctgatggtat tatgcaaaaa ggatttgtta
     6961 atataaatga taagacattc tattttgatg attctggtgt gatgaagtca ggatatactg
     7021 aaatagatgg aaaatatttt tactttgctg agaatggaga aatgcaaata ggagtattta
     7081 atacagcaga tggatttaaa tattttgctc atcatgatga agatttagga aatgaagaag
     7141 gtgaagcact ttcatattct ggtatactta attttaacaa taagatttat tattttgatg
     7201 attcatttac agcagtagtt ggatggaagg atttagaaga tggttcaaaa tattactttg
     7261 atgaagatac agcagaagca tatataggta tctcaataat taatgatggt aaatattatt
     7321 ttaatgattc tggaatcatg caaattggat ttgtcacaat aaataatgaa gtattttatt
     7381 tctctgattc tggaatagta gaatctggaa tgcaaaatat agatgataat tatttctata
     7441 tagacgaaaa tggtctagtt caaattggtg tatttgacac ttcagatgga tataaatact
     7501 ttgcaccagc taatactgta aatgataata tctatggaca agcagttgaa tatagtggtt
     7561 tagttagagt tggtgaagat gtatattatt ttggagaaac atatacaatt gagactggtt
     7621 ggatatatga tatggaaaat gaaagtgata aatattattt cgatccagaa actaaaaaag
     7681 catataaagg tattaatgta attgatgata taaaatacta ttttgatgag aatggaataa
     7741 tgagaacagg tcttataaca tttgaagata atcattacta ttttaatgaa gatggtatta
     7801 tgcaatatgg ttatctaaat atagaagata agacgttcta ctttagtgaa gatggtatta
     7861 tgcagattgg agtatttaat acaccagatg gatttaaata ttttgcacat caaaatactt
     7921 tagatgagaa ttttgaggga gaatcaataa actatactgg ttggttagat ttagatgaaa
     7981 agagatatta ttttacagat gaatatattg cagcaactgg ttcagttatt attgatggtg
     8041 aggagtatta ttttgatcct gatacagctc aattagtgat tagtgaatag ataaaaatac
     8101 gttaaatata tcctcttata cttaaatata taaaaataaa caaaatgata cactacataa
     8161 agtgttctat ctaatatgaa gatttaccaa taaaaaggtg aactatgatg aatgcacagt
     8221 agttcacctt tttatatttc taatggtaac aaaatatttt tttatataaa cctaggaggc
     8281 gttatgaata tgacaatatc ttttttatca gagcatatat ttataaagtt agtaatttta
     8341 actatatcat ttgatacatt attaggatgt ttaagtgcaa taaaaagtcg taaatttaat
     8401 tctagttttg gaatagatgg aggaatcaga aaagtagcaa tgatagcatg tatatttttt
     8461 ttatcagtag ttgacattct tacaaagttt aactttttat ttatgttacc acaagattgt
     8521 atcaattttt taagactaaa acatcttgga atatctgaat ttttctctat tttatttatt
     8581 ttatatgaaa gtgtaagcat attaaaaaat atgtgcttat gtggattacc agtacctaag
     8641 agattaaagg aaaaaatagc agttttacta gatgcaatga cagatgaaat gaatgctaag
     8701 gatgaaaagt aagtaatggt agatataata aaaatattaa caaataaaaa gtgttatcca
     8761 aataagaata gctgaaagtt atcataattc atgaaactga taatgaaaac gagggagcag
     8821 atgccaagag acacacaagt attaaataca tataatttcg aagcaagtgt tcattactat
     8881 atggatgaca aggtagtata tcaaacattg gttcacaaag atggtgcatg gtcagttggt
     8941 aaaatctatt aagctacatt agttacagat atcacaaact ataatagtta aacatagaaa
     9001 tatgtgtaaa ttgtgatgga aattattcaa aaacacaaaa atacgtgatg aaggacaaaa
     9061 tgatagagaa aataagtatc aaaccttaat aaatgattta attgatagtt taaaagttat
     9121 aggaaaaata tataaagaaa taaaaacatt aaaaaaatat aagatatgtt tacaaattac
     9181 tatcagacaa tctccttatc taatagaaga gtcaattaac taattgagta tctttaaatt
     9241 gaaatgttag gaagtgattt aaatatgaaa acttaaatta taaaaaatca atattaattt
     9301 atctttaaaa aatagaaagg agtgtataag atttattttc aaagtttaaa aacaagaaaa
     9361 tcaatttaaa tttcagaagg aataaatgtg gttatagaag tggatttatt atcaaaaata
     9421 atagtactag gaggttttta tgtctttaat atctaaagaa gagttaataa aactcgcata
     9481 tagcattaga ccaagagaaa atgagtataa aactatacta actaatttag acgaatataa
     9541 taagttaact acaaacaata atgaaaataa atatttacaa ttaaaaaaac taaatgaatc
     9601 aattgatgtt tttatgaata aatataaaaa ttcaagcaga aatagagcac tctctaatct
     9661 aaaaaaagat atattaaaag aagtaattct tattaaaaat tccaatacaa gtcctgtaga
     9721 aaaaaattta cattttgtat ggataggtgg agaagtcagt gatattgctc ttgaatacat
     9781 aaaacaatgg gctgatatta atgcagaata taatattaaa ctgtggtatg atagtgaagc
     9841 attcttagtc aatacactaa aaaaggctat agttgaatct tctaccactg aagcattaca
     9901 gctactagag gaagagattc aaaatcctca atttgataat atgaaatttt acaaaaaaag
     9961 gatggaattt atatatgata gacaaaaaag gtttataaat tattataaat ctcaaatcaa
    10021 taaacctaca gtacctacaa tagatgatat tataaagtct catctagtat ctgaatataa
    10081 tagagatgaa actttattag aatcatatag aacaaattct ttgagaaaaa taaatagtaa
    10141 tcatgggata gatatcaggg ctaatagttt gtttacagaa caagagttat taaatattta
    10201 tagtcaggag ttgttaaatc gtgggaattt agctgcagca tctgacatag taagattatt
    10261 agccctaaaa aattttggcg gagtatattt agatgttgat atgcttccag gtattcactc
    10321 tgatttattt aaaacaatac ctagacctag ctctattgga ctagaccgtt gggaaatgat
    10381 aaaattagag gctattatga agtataaaaa atatataaat aattatacat cagaaaactt
    10441 tgataaactt gatcaacaat taaaagataa ttttaaactc attatagaaa gtaaaagtga
    10501 aaaatctgag atattttcta aattagaaaa tttaaatgta tctgatcttg aaattaaaat
    10561 agctttcgct ttaggcagtg ttataaatca agccttgata tcaaaacaag gttcatatct
    10621 tactaaccta gtaatagaac aagtaaaaaa tagatatcaa tttttaaacc aacaccttaa
    10681 cccagccata gagtctgaca ataacttcac agatactact aagatttttc atgattcact
    10741 atttaattca gctaccgcag aaaactctat gtttttaaca aaaatagcac catacttaca
    10801 agtaggtttt atgccagaag ctcgctccac aataagttta agtggtccag gagcttatgc
    10861 atcagcttac tatgatttca taaatttaca agaaaatact atagaaaaaa ctttaaaagc
    10921 atcagattta atagaattta aattcccaga aaataatcta tctcaattga cagaacaaga
    10981 aataaatagt ctatggagct ttgatcaagc aagtgcaaaa tatcaatttg agaaatatgt
    11041 aagagattat actggtggat ctctttctga agacaatggg gtagacttta ataaaaatac
    11101 tgccctcgac aaaaactatt tattaaataa taaaattcca tcaaacaatg tagaagaagc
    11161 tggaagtaaa aattatgttc attatatcat acagttacaa ggagatgata taagttatga
    11221 agcaacatgc aatttatttt ctaaaaatcc taaaaatagt attattatac aacgaaatat
    11281 gaatgaaagt gcaaaaagtt actttttaag tgatgatgga gaatctattt tagaattaaa
    11341 taaatatagg atacctgaaa gattaaaaaa taaggaaaaa gtaaaagtaa cctttattgg
    11401 acatggtaaa gatgaattca acacaagcga atttgctaga ttaagtgtag attcactttc
    11461 caatgagata agttcatttt tagataccat aaaattagat atatcaccta aaaatgtaga
    11521 agtaaacttg cttggatgta atatgtttag ttatgatttt aatgttgaag aaacttatcc
    11581 tggtaagtta ctattaagta ttatggacaa aattacttcc actttacctg atgtaaataa
    11641 agattctatt actataggag caaatcaata tgaagtaaga attaatagtg agggaagaaa
    11701 agaacttcta gctcactcag gtaaatggat aaataaagag gaagctatta tgagcgattt
    11761 atctagtaaa gaatacattt tttttgattc catagataat aagctaaaag caaagtccaa
    11821 gaatattcca ggtttagcgt caatatcaga agatataaaa acattattac ttgatgcaag
    11881 tgttagtcct gatacaaaat ttattttaaa taatcttaag cttaatattg aatcttctat
    11941 tggtgattac atttattatg aaaaattaga acctgttaaa aatataatcc acaattctat
    12001 agatgattta atagatgagt tcaatctact tgaaaatgta tctgatgaat tatatgaatt
    12061 aaaaaaatta aataatctag atgagaagta tttaatatct tttgaagata tctcaaaaaa
    12121 taattcaact tattctgtaa gatttattaa caaaagtaat ggtgaatcag tttatgtaga
    12181 gacagaaaaa gaaatttttt caaaatatag cgaacatatt acaaaagaaa taagtactat
    12241 aaagaatagt ataattacag atgttaatgg taatttattg gataatatac agttagatca
    12301 tacttctcaa gttaatacat taaacgcagc attctttatt caatcattaa tagattatag
    12361 tagcaataaa gatgtactga atgatttaag tacctcagtt aaggttcaac tttatgctca
    12421 actatttagt acaggtttaa atactatata tgactctatc caattagtaa atttaatatc
    12481 aaatgcagta aatgatacta taaatgtact acctacaata acagagggga tacctattgt
    12541 atctactata ttagacggaa taaacttagg tgcagcaatt aaggaattac tagacgaaca
    12601 tgacccatta ctaaaaaaag aactagaagc taaggtgggt gttttagcaa taaatatgtc
    12661 attatctata gctgcaacgg tagcttcaat tgttggaata ggtgctgaag ttactatttt
    12721 cttattacct atagctggta tatctgcggg aataccttca ttagttaata atgaattaat
    12781 attgcatgat aaggcaactt cagtggtaaa ctattttaat catttgtctg aatctaaaga
    12841 atatggccct cttaaaacag aagatgataa aattttagtt cctattgatg atttagtaat
    12901 atcagaaata gattttaata ataattcgat aaaactagga acatgtaata tattagcaat
    12961 ggagggggga tcaggacaca cagtgactgg taatatagat cactttttct catctccata
    13021 tataagctct catattcctt cattatcagt ttattctgca ataggtataa aaacagaaaa
    13081 tctagatttt tcaaaaaaaa taatgatgtt accaaatgct ccttcaagag tgttttggtg
    13141 ggaaactgga gcagttccag gtttaagatc attggaaaat aatgggacta aattgcttga
    13201 ttcaataaga gatttatacc caggcaaatt ttactggaga ttctatgcct ttttcgatta
    13261 tgcaataact acattaaaac cagtgtatga agacactaat actaaaatta aactagataa
    13321 agatactaga aactttataa tgccaactat aactactgac gaaattagaa acaaattatc
    13381 ttattcattt gatggagcag gaggaactta ctctttatta ttatcttcat atccaatatc
    13441 aatgaatata aatttatcta aagatgattt atggatattt aatattgata atgaagtaag
    13501 agaaatatct atagaaaatg gtactattaa aaaaggaaat ttaatagaag atgttttaag
    13561 taaaattgat ataaataaaa ataaacttat tataggcaat caaacaatag atttttcagg
    13621 tgatatagat aacaaagata gatatatatt cttgacttgt gagttagatg ataaaattag
    13681 tttaataata gaaataaatc ttgttgcaaa atcttatagt ttgttattgt ctggggataa
    13741 aaattatttg atatccaatt tatctaatac tattgagaaa atcaatactt taggcctaga
    13801 tagtaaaaat atagcttaca attacactga tgaatctaat aataaatatt ttggagctat
    13861 atctaaaaca agtcaaaaaa gcataataca ttataaaaaa gacagtaaaa atatattaga
    13921 attttataat ggcagtacat tagaatttaa cagtaaagac tttattgctg aagatataaa
    13981 tgtatttatg aaagatgata ttaatactat aacaggaaaa tactatgttg ataataatac
    14041 tgataaaagt atagatttct ctatttcttt agttagtaaa aatcaagtaa aagtaaatgg
    14101 attatattta aatgaatccg tatactcatc ttaccttgat tttgtgaaaa attcagatgg
    14161 acaccataat acttctaatt ttatgaattt atttttgaac aatataagtt tctggaaatt
    14221 gtttgggttt gaaaatataa attttgtaat cgataaatac tttacccttg ttggtaaaac
    14281 taatcttgga tatgtagaat ttatttgtga caataataaa aatatagata tatattttgg
    14341 tgaatggaaa acatcgtcat ctaaaagcac tatatttagc ggaaatggta gaaatgttgt
    14401 agtagagcct atatataatc ctgatacggg tgaagatata tctacttcac tagatttttc
    14461 ctatgaacct ctctatggaa tagatagata tatcaataaa gtattgatag cacctgattt
    14521 atatacaagt ttaataaata ttaataccaa ttattattca aatgagtact accctgagat
    14581 tatagttctt aacccaaata cattccacaa aaaagtaaat ataaatttag atagttcttc
    14641 ttttgagtat aaatggtcta cagaaggaag tgactttatt ttagttagat acttagaaga
    14701 aagtaataaa aaaatattac aaaaaataag aatcaaaggt atcttatcta atactcaatc
    14761 atttaataaa atgagtatag attttaaaga tattaaaaaa ctatcattag gatatataat
    14821 gagtaatttt aaatcattta attctgaaaa tgaattagat agagatcatt taggatttaa
    14881 aataatagat aataaaactt attactatga tgaagatagt aaattagtta aaggattaat
    14941 caatataaat aattcattat tctattttga tcctatagaa tctaacttag taactggatg
    15001 gcaaactatc aatggtaaaa aatattattt tgatataaat actggagcag cttcaactag
    15061 ttataaaatt attaatggta aacactttta ttttaataat aatggtgtga tgcagttagg
    15121 agtatttaaa ggacctgatg gatttgagta ttttgcacct gccaatactc agaataataa
    15181 catagaaggt caggctatag tttatcaaag taaattctta actttgaatg gcaaaaaata
    15241 ttattttgat aatgactcaa aagcagtcac tggatggagg attattaaca atgagaaata
    15301 ttactttaat cctaataatg ctattgctgc agtcggattg caagtaattg acaataataa
    15361 gtattatttc aatcctgaca ctgctatcat ctcaaaaggt tggcagactg ttaatggtag
    15421 tagatactac tttgatactg ataccgctat tgcctttaat ggttataaaa ctattgatgg
    15481 taaacacttt tattttgata gtgattgtgt agtgaaaata ggtgtgttta gtggctctaa
    15541 tggatttgaa tatttcgcac ctgctaatac ttataataat aacatagaag gtcaggctat
    15601 agtttatcaa agtaaattct taactttgaa tggtaaaaaa tattactttg ataataactc
    15661 aaaagcagtt accggatggc aaactattga tagtaaaaaa tattacttta atactaacac
    15721 tgctgaagca gctactggat ggcaaactat tgatggtaaa aagtattact ttaatactaa
    15781 cactgctgaa gcagctactg gatggcaaac tattgatggt aaaaaatatt actttaatac
    15841 taacacttct atagcttcaa ctggttatac aattattaat ggtaaatatt tttattttaa
    15901 tactgatggt attatgcaga taggagtgtt taaagtacct
//
LOCUS       DAELSY010000091        15837 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.091, whole genome shotgun sequence.
ACCESSION   DAELSY010000091 DAELSY010000000
VERSION     DAELSY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15837
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.091"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            581..1231
                     /locus_tag="KPG09_002508"
     CDS             581..1231
                     /locus_tag="KPG09_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0967109.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            1509..3575
                     /locus_tag="KPG09_002509"
     CDS             1509..3575
                     /locus_tag="KPG09_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG0967110.1"
                     /translation="MKEVVESLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYVWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYIYDSNINKLKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNICILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSSMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            3592..5547
                     /locus_tag="KPG09_002510"
     CDS             3592..5547
                     /locus_tag="KPG09_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG0967111.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKEAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDR
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGIDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLERSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            5563..6753
                     /locus_tag="KPG09_002511"
     CDS             5563..6753
                     /locus_tag="KPG09_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG0967112.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(7601..7784)
                     /locus_tag="KPG09_002512"
                     /pseudo
     CDS             complement(7601..7784)
                     /locus_tag="KPG09_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427897.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7887..8261
                     /locus_tag="KPG09_002513"
     CDS             7887..8261
                     /locus_tag="KPG09_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0967113.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFVSTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMGILYEED"
     gene            8263..9120
                     /locus_tag="KPG09_002514"
     CDS             8263..9120
                     /locus_tag="KPG09_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967114.1"
                     /translation="MENILEVYNLNKTYTNFSLSNISFVLPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMSEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTEYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDEIKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            9123..9761
                     /locus_tag="KPG09_002515"
     CDS             9123..9761
                     /locus_tag="KPG09_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG0967115.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVMIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            10735..11469
                     /locus_tag="KPG09_002516"
     CDS             10735..11469
                     /locus_tag="KPG09_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0967116.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEVSYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMVLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELTPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRDIIGTLKNLIIE"
     gene            12611..13867
                     /locus_tag="KPG09_002517"
     CDS             12611..13867
                     /locus_tag="KPG09_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG0967117.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFLLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGIAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKTLGLEKFS
                     FALSSKVVFDLPIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            13930..15123
                     /locus_tag="KPG09_002518"
     CDS             13930..15123
                     /locus_tag="KPG09_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG0967118.1"
                     /translation="MLTSLALIFLLGMASGGIFEKIKLPSLLGMLLTGIILGSYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMNIGIFLQIKYRVVARRLSVKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGIASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     EGKSK"
     gene            15330..15710
                     /locus_tag="KPG09_002519"
     CDS             15330..15710
                     /locus_tag="KPG09_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0967119.1"
                     /translation="MYLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKILSKLSKKRFLYVQETGKCMYYTVAIKEKTYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYI"
BASE COUNT         5870 a         1748 c         2784 g         5435 t
ORIGIN      
        1 tcgtatttca aattgataat acaagaaaat aaaaaataaa aaattttaaa ttttttattt
       61 attgcgaaaa aaataaaaag gtgctatact ttttgtaaca tattattcaa attattaaga
      121 gatagtaaat atttatagtt taatttgtag tacactagca tgatttgcag aacaatgaat
      181 taatctatag tataatagtt taatttgaag tacaacgatt taatctatag tacactagca
      241 tgatttgaag tataacgatt taatctatag tacaataact tgatttgcaa tacaataatt
      301 taatctatag tataatagca tgatttgaag tataacgatt taatctatag tacaataact
      361 tgatttgaag tacaacgatt taatctatag tacaataact tgatttgcaa tacaatgatt
      421 taatctatag tataatagca tgatttacag tacaataatt taaattgtag tacaaaagta
      481 ctatcaaata agaatttcta ctttaaaaat attactaaaa gtaattaaaa tccaataatt
      541 caatataata tggcaaaaga atagaaataa ggtgagagta gtgaaagtaa agtcaaattt
      601 agatgaaatt ttatatcata agattattga aagtcttatt cgtggtgaat actctgtagg
      661 tcaaaaaata ttactaaatg acctttgtga aaaatttgaa gtaagcagaa caccagttgt
      721 acaagcagta aaaatgctaa ataaagatgg agtgcttact attatgacaa atggtaaagt
      781 gtacgtacca gagtatgaat atgatatggt aaaacaagtt tgtgaaacga gaacactgat
      841 tgaaacatat gctttagaaa aaatgatgca agaagaggaa gaggtttttc aacaaaagct
      901 agatgtaata aaaagatact ctgataaatg cgaggagtac tatcgtcaag aaaaatctgt
      961 agaacttgca ctagcagatt tggaattaca taaagccatt gtagaagggg caaataatga
     1021 gattttaaat gatgtatatg ttggtataca aggaagattt atagtagtga actatttaat
     1081 tcgaccatta aaaaatcgta attatgaagg aacagtacaa gaccattttg aaatattaaa
     1141 attcattgaa gacagagata caaaaaaagc agtagataaa ctaagaaatc atattcaagg
     1201 aactattaaa agattctgtg aagatgaata ataagttttg aagttactat atacttatct
     1261 attaagttgc taaatgtagt ctaagtataa tctaattata gtttaaacat aatctaaata
     1321 taatctaaat atagtgtaaa taagtctaag tatgaattaa atatagatta agtatgaatt
     1381 aagcatggat taaatatact ttaaattaca aatattacaa ggaagccaga aggcttccta
     1441 aaaaaataac ctttataaaa ttttttattt taaatttaaa attttttatt ttaaatttaa
     1501 aataaaaaat gaaagaggtg gtagagagtt tgaaaaaaat tactaaagaa aggatagaaa
     1561 atactatgaa aaagctattt acgagcgaaa catttttgga atatcaattt ataagtgatt
     1621 gccaaatatc accagatgga ggatatacag catttattgt aaaaaaagct gatatcaaag
     1681 agaatggtta cacaagccaa gtttatatat taaacaataa aactggcgaa ttaaaacaaa
     1741 taacgtctat aaactctgtt ggagcatatg tctgggaaga tgaaaatact atcttgttcc
     1801 ctgctttgcg aaatgaaaaa gtaaaagaag ctgtcaaaaa cggtaagcaa tgcatgagtt
     1861 actatgcttt atctttaaat ggtggagaag cagaagaact ttttagactc cctatcaaag
     1921 gtggtaaatt gaatcctata ggaaaaggtt tgtatgcagt tattgattct tatgacaatg
     1981 accgacctat tgtagaggga ttgccagaag aagaacaaca gaaaaaaata aatgcttata
     2041 acaaaagaca ttatgaacat ttcaaagaaa ttccttatgc tgtcaatggg gagggatata
     2101 ttagcagaaa aagaaaacgc ctatatatat acgacagtaa tataaataag ttgaaggcaa
     2161 ttacagcacc tatgtttaat gttgtaggta tgaaaataag tgatggaaaa atcttatata
     2221 tagggcaaga gtttaaagat gtaaaaggtt tgaaaaatgg ggtctatgta tttgatacaa
     2281 acacaaacac aaatatctgt atattagaaa aagataagta tattataaaa ggttttgaat
     2341 tatatcaaaa tcaagtgata ctaaacctaa ctgatgcttt atcttatgga aatggagaaa
     2401 atggagattt ttatactatt gatattgata cgaaagaaat gaaacttctt tcagggcatc
     2461 aacatcattg tattggaaat acagtaacat ctgatgttaa aatgggtgct ggtcagacaa
     2521 caaaggtaga tggagaatat atttattaca cttctacagt agatatggac tgcattattg
     2581 aaagaataca tattccaaca ggaaaacagg aaaaagtaac acagacaggt tcagttgatt
     2641 ttatagatgt aaaagatggc aatattgtat gtgtgggttg tttaggaaat ggattaccag
     2701 aagtttatac agtagagaat ggaacattgt gtaagaaaac acatttaaat gaccatattt
     2761 tagaggaata taaaatttct gtgccagaat acatagagtc aaaaggaagt tctaaatggg
     2821 aaatacaagg atacgtatat aaaccagtag attatgaggt ggggaaaaaa tatccagctg
     2881 tattggcgat tcatggagga ccaagattga catatggacc atattttatg catgagatac
     2941 aggtatttac aagtgcaggt tactttgtat tcttctgtaa cccaaggggg tctgaaggtc
     3001 gtggcaatgc ttttgcagat attaggaaac agtttggaga tattgattat atagatttta
     3061 tggaatttac agatactgta cttgaaaaat atacagacat tgataaaaca aaattggctg
     3121 ttgaaggtgg aagttatggt ggatttatga cgaattggat aattggtcat acaaaccgtt
     3181 ttgctgttgc ttgtgctcag cgttctattg caaactggag tagcatggag ggtacaacag
     3241 acattggtta ttatttctgc aaaggacaaa caggtgcttc tcatatggaa aaccacgagc
     3301 ttcaatggaa acagtcacct cttgcttatg ctgataaatg tgttacaccg actttatttt
     3361 tacatggtga aaaagactat agatgctata tgcaagaagc attccagatg ttttcagcat
     3421 tgaaaattca tgggtgtcct acaaaacttt gtctatttga aggggaaaac catgaactgt
     3481 ctcgttctgg tagaccaaaa caaaagctac aaagattagt tgaaatgcta gattggtttt
     3541 ctgtgtacat aaaaaaagat aacataggac agtagaaagg agaatataaa aatggaaaaa
     3601 ataaagattg aaagttcaga tatttttgaa tatatattcc ctcatgatat ttcttgttca
     3661 cctgatggaa agcatattgc ttatattata tcaaatatca acgaggaaaa agactgctat
     3721 gaacatgact tatatgtgat ggacatcaaa acagaaaaac aaatacatat gactcaaaca
     3781 aaagatgtaa catcatttag ttggatttct aatacagaac tactttttac atctaaaaga
     3841 aataagccaa aagcaggtac aacagatttt tacactattt ctatagaagg aggagaagca
     3901 aaagaagcat tttctattcc aaaagcttgt agcgtacctg tatcacttgg aaataaattg
     3961 tggttattga caacaaaaaa tccaacggat tctaaaaaat cagaaccaga tagggcagta
     4021 gagggtgtag attattggac atttacagat aaacctttta taagagatag agaaaatttt
     4081 tctcaaagaa gacgtgtaac attagaatta tatcaagaag gtgaaaatat aacaaaagcc
     4141 attacaccaa aattttgtga agtagcaggt atagacgttt cttctgataa aaacagaatt
     4201 ttatatactg gtcagattta tgaagattgt gcaactcctt tttctggact ttgggagtat
     4261 catatagaca gtggtgaaac caaagaactt gtaccacaag gaaagtacca aataagtttg
     4321 gcaaaatata ttggcaaaga taaagtaatg ttgcaagctt caacattaga acgttctatc
     4381 acacagaatc atgatatttt tatacttgat ttgagtacta gtgaaataaa tatgattgct
     4441 agtccagatg gtatgtatgc tacattactg gatgtagatg cagtatatgg tggtggacgt
     4501 agtaataaag ttataggaga taaatttatt ggtgcaagaa tttgcagaac tatgacagaa
     4561 tttaatgagt ttgatacaaa gacaggtaat atacgtatca tcacaaaagt agatgctttt
     4621 acatcttttg atatttacga caacaccatg tacacagtaa tgttgaaaga ttatgagtta
     4681 gcagaaatat atagcattga tatgactaca ggtacaatga aaaaaatgac tgctttttct
     4741 aaaccatatt tagatactca taaggtttca ttacctgaaa aattgacgtt tgtagcaaaa
     4801 aataaagaag aagttgatgg ttttgttata ccacctatag atgctaaaga aggcgaaaaa
     4861 tatcctgctg tattgttcat tcatggtgga cctaaatggg catatggcta tatgtttaca
     4921 cacttgaagc agtgtgtaac atcaaaggga atgtatgtga tttattgtaa ccctcatggt
     4981 ggtgacggtt atggtgaaaa attcttagaa atggtagaaa gatgggggta tgttgactat
     5041 gagcatctta tggaatttgt agatacttgt attgaaaaat atcctgggat tgatgcagac
     5101 cgcttagggg ttgcaggggg aagttacggt ggttatatga caaactggat aattggccat
     5161 acagaccgtt tcaaagcagc agtttctcaa agaggtatta gtaacttgat tacagcttct
     5221 ttaattattg actttgggga tagaattatg aagcagactt gtggagataa gacgccttgg
     5281 aatcatgagg aggttctttg gaatcattca cctattaaat atgtgaaaaa tgttaagaca
     5341 ccaacattgt tcttacatag tgatagggat tatagatgtt ttatggggga tactttccaa
     5401 atgtttacag cattaaaaca gcttggtgta gatacagaaa tgtatttgtt ccatggagat
     5461 acacatggat tgtctagaaa tggaagacca tctaatagaa ttgcacgagc aaatgctatt
     5521 gtagattggt ttgaaagata tttataataa gggggttatt ctatgagtga tatgttatta
     5581 tggtttagtg tcgtattagt tttatttgct attggggatt taattgcaag caaaacaaaa
     5641 gcaaaagtat cagctgtatt tgttacacta cttttatttt tgattctatt tgtcacaaaa
     5701 gcaataccag ctgatattat tgaaaaagca ggtatgaccg cagcagctag ttggagtgtt
     5761 ccaatgatta tgtttagtat gggaacaatg ttgaatgtta aacaatttat agacgaatgg
     5821 agaacagtat tgacagcatg gcttggaatt gtagcagtta tagtctgtgt ctcattatgt
     5881 attcctttgt ttggaaaatc aacagtattg acaagcatac cagttataaa tggagcttta
     5941 cctgctacta ctattatgac acaagcagca ctagaaaaag gactgacact tgcagcagct
     6001 acagcaacag ttgtatttgc aattcagaag tttattggta caccaatagc ttcaagagca
     6061 gcgttacaag aggcaaaccg tcttttagta gaatatcatg aagcaaaatc aaaaggcatt
     6121 gatttagcaa atgttgatac tgataagaaa gaagcagaga atacaggcac aaaagtaaaa
     6181 caagctttct gtgaaaaata cgataaatat tattctacaa atgtttgtat attttttata
     6241 gcactattct cttatctagg gtatgaactt tcagagatta ttcatgtaaa ttattctatt
     6301 gtatgtcttg ttgttggtgt tattgtaaca cgtattggta ttgtaccaaa agacattttg
     6361 gaaaaaggaa aaatcaaggg atttattaac atggttgtgt ttgcagctgt tattccttct
     6421 ttagcaaaag tttctttgac agatttaatt agtctttttg taccaattgt gggtatgttt
     6481 gcagcttcta tcattggtat attccttatg atgaaggtgt taccaggttg gaaaattatc
     6541 ggttctaaac ctcttgcttt tggagtaggg ttttgtcaga tgttgggatt cccaactaca
     6601 tatttaattt caaatgaagt gtgtaatgct gttggagaaa cggaagaaga aagagcatat
     6661 ttgatgtcta agattatgcc taagctggta gttggtggta tggcttgtat gatttctatt
     6721 gtagtagctg gtcttatggt accaatgttg tagataggtt tcatagttaa aaagtggtgt
     6781 taatttatag gagaagctaa aaagtagtat taatttataa aataatttta tattagttaa
     6841 tttgttataa gaatattttc tttttatgat tataattttg attttataag agagcagatt
     6901 ttttatttgc tatgctaata gttataattt gtttttattt gctaaaatgc aactttaatt
     6961 tgatttatgt ataatatagg gtatttaata atatttttga agaattttta taaaaaatta
     7021 aaaagaggag aaaaaaggga gtgccacaaa gtgaactttt tagttctaag gcactctttt
     7081 ttgtacgaaa tcgaatatat tttcaacgtt ttaaaagtga gaaagagact tatctctagt
     7141 ttgggatagc caaatttaat tattaaaatt attttttgtt ttatttatca aagagtattc
     7201 gtgttttaga agttagtatt ttttatttta tatatccaag gttttccgtt tctaatgttt
     7261 tcttttttaa aataattagg gtattcaatt atagtaaaag gtggatggta ttttggaatg
     7321 atattattta aagcacagta ttgaatgttg aaatcctcac attttagagt attatctttt
     7381 gtaatcaaaa aaatcttcta tacttatagc aaggttactt ctgcaaatat tccatgcatc
     7441 ataatatcca aatttaaatt tttttaatat agagtttacc tctagtctac actcttgaat
     7501 aactctttcc tctatccaaa ttcttataaa ctcatcccat ttattactat ctatttatta
     7561 ctatctaaat tttttagtat gtttgtaatt ggaaatggat ttattccttt atgtttttta
     7621 taaatagttt ataacttaat ttattattaa acaaatcatt catacagaat tactttttct
     7681 tttagatatt caattcttac ttttaataaa tatttaatac attctatctt atgtggtttt
     7741 aaaaagtgtt tatggctatc aataatctct aatatttcat tcatatttat tttgtcatag
     7801 taatatttta catcttttat attgacaaaa aagtgctcta agtgtatagt gtgtatataa
     7861 taacatcaca ctggaggtga taaaaaatga aagtgctcat ttctaacaca tcagataagc
     7921 cattatacca acaaattaaa gagcaaatta aagaagcaat atttactgaa gaactgaaag
     7981 aaggggatgc aatgccctca ataagaaatt ttgctaatga tttaaaagtt agtattttga
     8041 ctattagacg agtgtatgaa gaacttgaaa atgaaggttt tcttacacgc caagttggaa
     8101 taggaacatt tgtttcaact ggaaatttag aaatactaag agactcaaag aggcgtatag
     8161 ttgaacaaaa aatggcagat atgattaagg atgcaaaagc actaggaatt acacagatag
     8221 agttacaaga aatgatgggt attctatatg aggaggatta atatggaaaa tattttagaa
     8281 gtttataact taaataagac ttataccaat ttttcattat ctaatataag ctttgtatta
     8341 ccagaagatt gtataactgg atttataggt gttaatggag caggaaaaac aacaacaatt
     8401 cgttcgatat tggatgtcgt aaagcgtgat agtggaaaaa taaaaatctt tggtaaagat
     8461 ttcgatggaa atgagaagga attaaaaaat cgaattggag ttgtatttga tgattcatat
     8521 ttctatgagg agttgtcaat gtcagaaatg aaaagtatta ttgcaccttc atatgataat
     8581 tggtgtgaag aagattatca acaaaatatg aagcgtttta atcttaatcc aaaacaaaag
     8641 attgatacac tttcaaaagg aatgaaaatg aaatttgctt tagcattagc attatcgcat
     8701 aaagctgagt ttttaattat ggatgagcct acaagtggtt tagacccact aatccgtagt
     8761 cagatacttg acatactaac agaatatatg aagaaatgtg gtcgtggtgt tttcttttct
     8821 actcatataa catctgattt agacaaaata gcagatatga ttgttatgat tgacaatgga
     8881 cgtattatat tagagcgtga aaaagatact ctattggatg aacatagaaa agtaaaaggg
     8941 gatattcaat ttctaaatga tgaaatcaaa aaattatttt taaatttaga aattacaggt
     9001 tatggtttta tagggattac ctcaaaaatt aatgaagtgc aaaaaaatat gccagatatt
     9061 ttaatagagc gtcctacaat tgaagatatt atgcttgcat atattgaaag gagagagtaa
     9121 ttatgttgat tcatttagtt aaaaaagata ttctgattgt aaaaaaatat gtattgttga
     9181 tattgtttat tgcaatagga atcccattat ttatattatg gcgtttacct ccatttgcac
     9241 aaatattggg atttgtacta tcaattatat tttcagaatt tatgctgtgc cagtatctat
     9301 tattaaagga gagtcaatat tcaaaagcat ctgtattact ttgttcaact ccttatccta
     9361 gacgtggctt agttcaatct aagtatgttt tatttgcaat ggtatttatc tattgtacat
     9421 tagtttactg gattgaaaat ttacttattc cacaaatagg aacttttgac ttaacatata
     9481 ttttgattgt tttattaata agttctatta tttatggagt ttatatgcct attcaataca
     9541 aattaggata tgaaacaaca aaattctttt tcgtgatgat aatcatggct tcatcatttg
     9601 tattaccatt aactatagct cacagcaatt ttgaaattcc atttatatat cagtatccat
     9661 ccatgatagt gaatattgta ttgttccttt taagtttaat tatactgtta gcttcaatgt
     9721 ttatttctat taaaattttt agtaataagg agcttgtata aggtaagggg gaattttata
     9781 tgccacattt gccagattgg gctgttttac tttgtatctt acttccaata gtatataaag
     9841 cttgtcagat atttaaaagt aaaaaataga gtacatattt atattgccat ttttattgag
     9901 tgaagttaga tggtatgaat ttaaaaggtt acttgaaaat aaagttactt ggtgtgggag
     9961 agtaacatca taatattata gattatatgt ttcttgtgaa tcaatttgta ttttttaata
    10021 gataatatcc agtaatttta ttttccattg attattttca attatgagta tgattgtgtg
    10081 tattgtatac catcttttaa ggtgttttta aaaaattata tcgtcaaaat ataaatctaa
    10141 ggaatcctat aaataaagct tttcaatttt agaaaactaa taaaaagtag gaagttaata
    10201 aaaagaagct gtctcaaaat agagataagc ttctttttca ttgctgaaat gacgaaaata
    10261 taattgattt aatataaaaa agagtgcttt atgaactatt tattttgaac agtagataca
    10321 cttgcactca atttagttac tcctaagctt tatgaactaa aagtttattt tgaagcctat
    10381 ttttctaaat aatcatctat atacagtatt gcgtttcttt tatttattca taatttattt
    10441 tcttattaaa gtttttctat attactttac ttttaatttt atacgaatta tcaaaaataa
    10501 aatagttatt agaaaaaaat aaaagtaatt gcaaagattg caattactta cttctatatt
    10561 aattactata ccataaaata acaaaaaaat caagtcgagt aatccttcaa cttaagtgat
    10621 atactaggta ttaataattt ttaattaata aaatataact agaggaaaat ctacattttc
    10681 tcaatttctt taattaaatt gacctctaat taagtaatta taaggagaaa aattatgaat
    10741 ttagcaaagg gattagatgt tttaaaaatt tcatcaaatg tattcggtga agataaagtt
    10801 atgtatattc ctgtaatcta cacagaagat gatgcaacac ttattgacac aggtcttccg
    10861 ggtcaaggtg atttaataat tgatgctctt aataaaagta acacgagctt tgataggtta
    10921 aaaaatataa taattactca tcatgatata gaccatatag gaaacataaa ttatctaaga
    10981 gaaaaatcta agaataatat caaagtatat gcatacaaaa gtgaagtcag ttatataaca
    11041 ggagaagaaa ctccttttaa actttatatg ttggaacaaa tggtagataa aattgatgat
    11101 aagatgctta gtatgctaaa tgttatggta cttggattta aatcatcata tactaaagta
    11161 gatgtgtcat tagataacca tgaaaaattg aatctaggtg aggaaataga agttatacat
    11221 acaggaggtc acactagagg tcacatatgt ctttatttaa aagagtctaa agtacttatt
    11281 gctggagatt tgttacaggt agaaaatgga gaacttacgc ctgttgatgt aatgcacagc
    11341 aacaaacaag agttaaaaga tgctattaaa aatataagta attatgatat agaaacgata
    11401 gtttttagtc atggtggatt atatcaaaga gacataattg gaacattgaa aaacctaatt
    11461 attgagtagt actatatgat ttacaataat ttcttaaatt acttaaaatt atatctaaag
    11521 atgtagaatc aaaattaaat ataagtaata agaattttgc tttaaaatta tacttaagtg
    11581 ttaagtatga tacgtagtat tacaagtatt ttagaatata attttttata gtaattttaa
    11641 ggttctacaa tacttcagtt gaaaaatatt taacagcata gtataatagg aaagaaaata
    11701 ttttaaataa actttagtat acaaagtgaa gaaaaatatt taaattatat ataatattgt
    11761 agagtgaggt aaaatattta agttatatat cataatacag agtgaagaaa aatatttaga
    11821 taatgtacca taatacaagg tgagtaaaaa tatttaaagc atgtataata atgcaaagcg
    11881 aagaaaaata tttaaataat gtaccataat gcagagtgag taaaaatatt taaataatgt
    11941 gttataatac aaagcgaata agattattta aagcatgtat tataatataa agtgagtaaa
    12001 aatatttaaa tcatgtatca taatataagc agagtgaaga aaaatattta aataatatat
    12061 cataatgtag agtgaggtaa aatatttaag ttatgtatca taatacaaag taaagaaaat
    12121 catttaaatt atatatcata aaaataatat gaaatattga acactcataa tattggaggc
    12181 tttaatattt taattgatag gttacattat ttataacatt aaaaatacaa tttacataaa
    12241 atagacatat gaaaaataaa aaattatata ataattatgg gaatgaggtt ctcccttgga
    12301 tgataatatc ctaaactgcc aatgagctga tgacttctat gatatcatag atgccaccag
    12361 ctttttttgt tgtttaaatc gataaaatgg aggtgaatta cttactaaat ttttattttt
    12421 taaatttgaa gaaatattta attaatagaa aaaacagtag attatatgtt aattatttta
    12481 ttaagaatgg gttaagagta tacctatatc ctatcaattt aaaaaacaat tatgatagtc
    12541 attacattga ctacagttga tgcaataaaa ctttcaaaaa atcttatgat gcaaatagaa
    12601 aggaattaaa atgatagaaa aatttaaaac tttaatatgg attaaaatga taataagaat
    12661 taaaagcgtt tataaaacat atggtggact atttttgtta ggactcatag gtataccagt
    12721 tggtgcaatt ataggtctta tagacaccat atttggaacg gttcttctaa aagttacaga
    12781 tattcgagaa acatatccaa tgtatctgat accattttta gcagtagtag gagttgttat
    12841 tgcttattgc tattttaaat ttggaggaaa aagtagtaaa ggaatgaatc taatatttga
    12901 ggtagggcat ggagaagaag aaattattcc tttaagattg gttccattta taatatcagg
    12961 tacatggtta acccatttat ttggtggtag tgcaggacga gaaggagttg ctgttcagat
    13021 tggtgcaact ttttcacatt gggtaggtaa aaggttgcct ataaaaaatg cttctagtat
    13081 ttttctagta actggtatag cagctggttt tgcaggatta tttgagacac caatagcagc
    13141 aatactattt gctatggagg ttttggtagc tggttcactg gagtatcaat cgctttttcc
    13201 tgcgtttact gcttctttta cagcaagtgc agtatcaaaa actttagggt tagagaaatt
    13261 ttcttttgca ctttcttcta aagttgtatt tgacttacca atattttgga agcttattgt
    13321 tttaggaatt atttttggaa tggttggtgg agcatttgca tggtgcctga aattgtcaaa
    13381 aagaaaaata ggtaatagat taaaaaatcc tatgataagg attgctatta ttggagtatg
    13441 tcttagtgta ttattccttt tattttataa aggaagatat tcaggtttag ggacaaattt
    13501 aattcaaaat agtttttatg gtggagaaat ttattcattt gactggctgt taaagtttat
    13561 acttacaata ctgactttgt cagcaggttt tcaaggagga gaggttacac ctttgttttc
    13621 cattggagct agtcttggtg tattgttagc aggatttttt aatttgccaa ttgaattagt
    13681 tgcagcgctt ggctatgcta gcgtctttgg cagtgctaca aatacttttt ttgctcctgt
    13741 atttataggg gcagaagttt ttggatacag ctatttacct tatttctttg tagtatgcgc
    13801 tatttcatat atatttaata tggataaatc tatttattca ctacaaaaaa tttcaacgaa
    13861 acaataaata aaacaacata atgtatgatt ataagagaaa gaatattaga gaaaggatag
    13921 ggtaggaata tgttgacaag tttagctttg atttttttat taggaatggc ctcgggaggt
    13981 atttttgaga agataaaatt acctagctta ttgggaatgt tacttacagg aatcatattg
    14041 ggttcatatg ttttaaattt gattgataat tcaatactag atatttcttc tgaccttaga
    14101 aagattgcat tgattattat attaacaagg gctggattat ctttagatat aaacgatttg
    14161 aagaaagtgg gaagacctgc tgtacttatg tgctttattc cagctacttt tgaaattatc
    14221 ggaatgatag ttttagcacc aaaattacta ggcgtttcta tattggaagc agcagtcatg
    14281 ggtgcagtag taggagcagt ttctcccgca attattgtac ctaaaatgct taaattaatg
    14341 gaagaaggtt atggtacaga aaaaagtatt ccacagatgc ttttagcagg aacttctata
    14401 gacgatattt tcgtaattgt gatgtttaca gtattcacag gattggcaca aggaaatagt
    14461 atatcagcta ttagtttttt acaaattcca gtatcaatta tattaggtgt aatagttgga
    14521 gcagtgattg gaatctgttt agcagtattt tttaagaagg ttcatatgag agattctgct
    14581 aaaggggtgt tgcttttaag tatctcattt ttaatgataa gtttggagac tgctttagag
    14641 ggtattgtgc cattttctgg attattagca gttatgaata ttgggatttt ccttcagata
    14701 aaatatcgtg tagttgcaag aagattgtct gtaaagtatt caaaactatg ggttggtgct
    14761 gaaattctct tgtttgtttt ggtaggggca acagtagata taagttatgc atttaaagca
    14821 ggaattggag ctgtaatttt aatatttgga gttcttttat tccgtatggt aggagtattc
    14881 ttctgcctta ttaagacaaa tttgacaata aaagaaagat tgttctgtat gattggctat
    14941 attccaaagg ctacagtaca ggcagcaata ggaggtgtac cacttgcaat ggggatagca
    15001 tctggacaac ttattttgac attagcggtt ctcgctatac ttataactgc acctcttgga
    15061 gcttttggaa ttgatgtaac ttataaaaaa ttattaactt ctgtagaagg taagagtaag
    15121 taagaataaa aataaagatt attttaagtg ataaattata ttttggaaaa aactatatta
    15181 ttgagtacaa tgttagaaaa tcatttgagt gattacagaa ggtagaagtt tatatcggat
    15241 tttaaggcat gtgatgatta ttagaattga ttttgtgaaa ttgttattgt aatatttaat
    15301 tatatagata aagaagcgga gtgataacta tgtatttaaa aaaattatca aaattagagt
    15361 tagttattat gaaatttatt tggaatttag atataaaaac caattcatat gagattattg
    15421 attatatgaa agaagagcat aatttaccag aaaaagtggc tttaaaaatt ttatcaaaat
    15481 tatcaaagaa aagattctta tatgttcaag aaacaggcaa gtgtatgtat tatacagttg
    15541 caataaaaga aaagacatat ttagagttta tatcaagaaa tgtgcaaaat cttttgaaga
    15601 ataattttat aaggaattta ttagtatcat ttcacgaaga ggagctaaca gaggaaaaga
    15661 taaagtcgtt agaaaactgg gtagtaaatt gggaagaagc ttatatataa atgttattat
    15721 ttttattaca atttagataa ttttggtttt gtaagctctg caactatttt tagatggttg
    15781 cagagcttat ttttattttt tattccctta agcaatactt aaaagtaaac gcattta
//
LOCUS       DAELSY010000092        15791 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.092, whole genome shotgun sequence.
ACCESSION   DAELSY010000092 DAELSY010000000
VERSION     DAELSY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15791
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.092"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            79..780
                     /locus_tag="KPG09_002520"
     CDS             79..780
                     /locus_tag="KPG09_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBG0967120.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIINIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1051..1869
                     /gene="larE"
                     /locus_tag="KPG09_002521"
     CDS             1051..1869
                     /gene="larE"
                     /locus_tag="KPG09_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBG0967121.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNMDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKILGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLFNLGFSQFRVRM
                     HGEIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            1924..3072
                     /locus_tag="KPG09_002522"
     CDS             1924..3072
                     /locus_tag="KPG09_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBG0967122.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            3208..4596
                     /locus_tag="KPG09_002523"
     CDS             3208..4596
                     /locus_tag="KPG09_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967123.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYTTLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            4625..5323
                     /locus_tag="KPG09_002524"
     CDS             4625..5323
                     /locus_tag="KPG09_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0967124.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     ENDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            5326..6759
                     /locus_tag="KPG09_002525"
     CDS             5326..6759
                     /locus_tag="KPG09_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0967125.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            6781..7518
                     /gene="truA"
                     /locus_tag="KPG09_002526"
     CDS             6781..7518
                     /gene="truA"
                     /locus_tag="KPG09_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG0967126.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    7629..7836
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8089..9288
                     /locus_tag="KPG09_002527"
     CDS             8089..9288
                     /locus_tag="KPG09_002527"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBG0967127.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            9535..10581
                     /locus_tag="KPG09_002528"
     CDS             9535..10581
                     /locus_tag="KPG09_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HBG0967128.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DGGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDERIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPSKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            10689..11852
                     /locus_tag="KPG09_002529"
     CDS             10689..11852
                     /locus_tag="KPG09_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0967129.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKIIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAQAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SKNPSSTDLQEIAKEITDISKKDYEIFSMYTGDDNWYYMIQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(11941..12321)
                     /locus_tag="KPG09_002530"
     CDS             complement(11941..12321)
                     /locus_tag="KPG09_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBG0967130.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(12324..12983)
                     /locus_tag="KPG09_002531"
     CDS             complement(12324..12983)
                     /locus_tag="KPG09_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBG0967131.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(13089..14063)
                     /locus_tag="KPG09_002532"
     CDS             complement(13089..14063)
                     /locus_tag="KPG09_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG0967132.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(14103..14450)
                     /locus_tag="KPG09_002533"
     CDS             complement(14103..14450)
                     /locus_tag="KPG09_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967133.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(14668..15624)
                     /locus_tag="KPG09_002534"
     CDS             complement(14668..15624)
                     /locus_tag="KPG09_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBG0967134.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTIYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIQAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
BASE COUNT         6185 a         1684 c         2576 g         5346 t
ORIGIN      
        1 aataatagtg gttttaaaaa tctgacaaca aaaataaaac tttcaaaaaa ttaaatctaa
       61 aaagaatagg agataaatat gagtattgaa gatttatatg attttgaatg tgttcctttg
      121 aacaatgaga ttgatattga tgttttattt gaaaagatga tagaagatgg aaaagaaaaa
      181 gaatatacac caattatagt cattgatgaa aaagtagggc tattaagaaa aaatatagat
      241 atgattacta aagagtatgg ttctcttgaa aactatagag agtattgttt aacaaaatat
      301 aatgaaattg atgtaaatca attttttgat tttaggaaga gtgagatttc agaattatta
      361 aaggagctaa ttgatttaga agatgatttt tatgattata aacattttag accaattata
      421 aatatagata tatttcatga atataacaaa gtttacatag ctaaaattcc aacaattaaa
      481 ccatatgaag tatttgcata catacctatt ggaggattta atgattgtcc tagagatgaa
      541 gaacatatag ctattgcaaa atattggtat gaaaagcatg gaatctttcc aatcgcaatt
      601 ggatgtgata cagttcaata ttctgtaaaa aatttaacta aagatggtaa aaaatttgat
      661 gacttatgta ttgaattagt attttattgt gaggatatta ttatacaagg gtatgaaacc
      721 ttaaaggctc ttaaggatgt attaaaacgc tctacaatag tgcttttttg gtgggattga
      781 tattctatca tttagatagt attaaattgt tagtcaccaa gaatccactt actttagtta
      841 tggggagtgt gtcaaactag gtttttatac acagtgtagc atagtaatat gtttaaatta
      901 caaaggttaa aaaaatactt taatattttt aatattataa ttaatagatt taaaggtaat
      961 tataatttat aaaataaata ctttatatgt tataatcatt aagaatgtaa aaaaataata
     1021 tatagtttta tagttcatat ttttccagat atggaggtaa attttgtgaa ggaaaaagaa
     1081 aaattagata aattaaaaaa aatgttactt gaattaggca gtgttgtagt tgcttattct
     1141 ggtggagtag atagtaattt tttgcttaag gtcgcaaaag atacattagg tgaaaatgtt
     1201 atagctgtga ccattcatgc aatgatgcat tcaagtaggg aaattgaaga ggctaaacaa
     1261 tatactcaaa attttggcgt taaacatatc atattaaata tagaaaactt tgatttaaaa
     1321 gaatttaaag aaaatggtgt tgatagatgt tatcattgta aaaaatatat attctctaaa
     1381 ataaaagagg tagcaaaaga acataatata aagtacatag tagatgggac aaatatggat
     1441 gatttaggag actatagacc tgggcttaag gcattgagtg aattaggagt gattagtcca
     1501 ttaaaagata gtagtttaaa aaaagaagaa attaggtcgc tttcaaaaat attaggatta
     1561 aatactttta ataaaccatc atttgcatgt cttgcaagta ggattcctta tggagttgag
     1621 ataacagatg aaaatttaag aatagtagaa aagagtgaag aatatctatt caatttaggt
     1681 ttttctcaat ttagagtgag aatgcatgga gagatagcta gaatagaagt tgggcaggaa
     1741 gaactaggta aattttttga aaataacaat tttaataaag ttgatactaa attaaaaata
     1801 tttggcttta aatatgttac actagatatg tcaggatata aaatgggaag tatgaattta
     1861 aatgtgtaga ttattttaaa aataaataag ttttatagtt aatataagga aacaggaggt
     1921 tttatgaaaa attatacatt aatatcgcca tgtttttttg gaatggaaaa aatgctggct
     1981 agagagatta ctaatttagg atatgaaata ataaaaacag aagatggaag aataacatat
     2041 aaaactgatg agtttggaat agcaaagtca aacatgtggt tgagatgtgc tgaaagagta
     2101 catcttaaaa ttgctgaatt tgaggcaaaa agctttgatg aattatttga aaatacaaaa
     2161 agaataaact ggtctagata tataccttat ggagctcaat ttcctatatc aaaagcttcc
     2221 tctataaaat caaaattata cagtacacca gatgtacaag ccatagtaaa aaaggctata
     2281 gtagaaagct taaagaaaag ttatttagag gatggtttgc ttaaagaaga taaggagaaa
     2341 taccctattt ttgtatttat acacaaagat aaagtaacca tttctataga tacaacagga
     2401 gatgctttgc ataagagagg atatagagaa aaggcaaata aagctccaat aagagagact
     2461 ttagctgctg ggcttattta tttaactccc tggaaagcag gtagagtttt agtagaccct
     2521 atgtgtggtt ctggtactat attgattgaa gctgctatga ttggtataaa tatggcacct
     2581 ggcttaaata gagagtttat atcagaaaag tggagaactc ttgataagaa aatatggtgg
     2641 gatgtaagaa aagatgcttt taataagata gataatgaat ctaaatttaa gatttatgga
     2701 tatgatatag atgaagaatc tatagatata gctagagaaa atgctgaaat agcaggtgta
     2761 gatgaatata tagagtttaa tgtaggagat gcaactcaat ttaaaagtga agatgagttt
     2821 ggatttataa tcactaatcc tccttacgga gaaagattag aagataaaga cagtgttaaa
     2881 cagttgtata aggagcttgg ttatgcattt agaaaattaa aaaattggtc gtattatttg
     2941 ataacttctt atgaagactt tgaatatgag tttgggcaaa aagcagacaa aaaaagaaaa
     3001 ttgtataatg gtatgttaaa aactaacttt ttccaatatc cgggtcctaa gcctcctaga
     3061 aataataaat aagttttaca tgatttattt gtaaaatata aaattttatt aaaataagta
     3121 aaacattatg atgccttgaa acgtctaatt attgtgagga gatttttgtg aacaaattta
     3181 aagatttata ggattaggag gagttaaatg aaagtgttaa agcttattac tgtagtagcc
     3241 ctgatgatgt tatttgttac tggatgtaat ataaaaactg aatctccaga acaattagcg
     3301 aagatacctg actatgatga tacaaaaaaa gtattgtatg atggtattga ccagttacta
     3361 aaaccagatt catctatgat attgccttct aatacaaaag aagtaggaaa aataaataag
     3421 gtagatttaa acagcgatgg aattgatgaa ttagtagtat ttgaacaaaa agaagattta
     3481 agtaataatg tgagtcaggt agggtttgta accttgagtt acaatggaga aaaatacgta
     3541 cttggagacc attttttaga aaatggcgaa tcaatagaat atgctaattt ttatgatttg
     3601 gactcagatg gatataagga agttatctta ttggttaaat ccaaagacaa aacgaatatg
     3661 catatatata aagtagtaga taatgaaata acaaaaatat atgacttgga tgcatcatgg
     3721 cttcaaaata aagaagattt taatgacatg aaagtaaaaa ttggatatat agatggagat
     3781 gacaagttag acatattgat gcttcatttg aataataaga ctaatgaaat gtttgcaagt
     3841 gttgcaaatt ttgatgggaa aatgaaaatc aaagattctg ttaagtttga aaatgtaaaa
     3901 aacttaagtg aattatatac aacattaggt aatgttgctt catctgttgg aaattcttct
     3961 gcagctataa aaggtattgt acttgatatt cctatattga aagataataa ctatattacg
     4021 cagatacttt atatgaaaga tgggaaaatt aacaaagctt ttagtgatta tgataaaact
     4081 attactaaat cttattatat acctgtagat gatattgaca aagataagat tatagaaata
     4141 cctatagttg caggcagtac aggtaataat aaaaatactt atagttctaa atcatctgca
     4201 actataagtt ggtatagatg gaatggaaaa gaaggtgctg actcaagttt aatatttaca
     4261 agtcagatat attataatta taaatataat tttaaattat ttattccaaa taatttattt
     4321 gataaaatac ttgttgaaca ggaatttgtt ggagaaaaag cagtatttaa attttactat
     4381 tttgactatt cagatcgtaa gaatttattt aatatagtag tagagagtaa aaacaaactt
     4441 gaagatagaa aaaatacagg tactcaaaac tctattgtac ttcaagagag cgatgaatat
     4501 actttcttac tagttgtaaa caatgcaaat gaaatggata agctagatat gactatagac
     4561 gcattaaaag aatatttctc attaatatat ggttaatatt atactcaatt ggaggtttta
     4621 ttttatgaag gaaaagatac ttatactaga agatgaaatt ggtattagaa gttttgttag
     4681 tataaactta aaaagagaag gctatgaaat agttgaggca ggtactggaa gagaagctat
     4741 agaaaaaatg acaacagaaa atgatattac aatagctctt ttagatgtga tgcttccaga
     4801 tataagtggt atagaagtat gtaaatttat aagggagaat tttgaccaag taggtataat
     4861 aatgcttact gctaaagctc aagaggatga taaaatagaa ggatttattt caggagcaga
     4921 tgattacatc ataaaaccat ttagtataaa agagctttta gtcagggttt ctgctcttct
     4981 tagaagagta gcaaaggatg attcaagtgt taagtcgagt gaaatagttt ctccaccatt
     5041 tatacttgat atagacaaga gaaaattatt taaaaatgga aaggaaattg agcttacacc
     5101 aactgaattt tcaatagtga aatatctaat atcaaatgct aagcaatcgt taagtagaga
     5161 ccagatatta gacgaagtat ggggaactaa ctatttatat gattttaaaa tagtagatgt
     5221 aaatataaga aggataagaa ataaaataga agatgaccca tcaaagccaa aatatataca
     5281 aactatttgg ggttatggat actgttttag aaaggaagaa taatagtgtt acattttgaa
     5341 ggtctgagaa aaaaagtaat aaaaaactat tttataataa ttattataat ggttactctg
     5401 tttgaaggat tgttcatgtt ttacatacaa aactactatt atgattctgt caaacaacta
     5461 cttgaatctg aaataaaata tgctgatgaa tataatgcta ttacaatgga aactacaagt
     5521 tttgagaaaa aagtaaaaaa tatatttgat aaacaaccat taactaaaaa ttcagaattt
     5581 ggaatttcaa taatagataa agataaaaat attatattag accaatatgg ttttaaaagt
     5641 aaagaaaaag caaactatga agatgttaac aatgcattaa aagatataaa aactaaaaat
     5701 ttaacacctt atacttatag aataccagat acaggggagc atgttatgag tatctcctta
     5761 ccacttaagg taaataatat aatagaaggt gttgttagat atacagtatc attagatgct
     5821 attgataatg caatattaaa gcaagctaca tggcttatat tagcaggaat atttatactt
     5881 attatagcta tactaataag tcttaaattt gctgagactc ttataaaacc attacgagag
     5941 ctaaagaaat ttgcaaatga attggctgta ggaaattata atataaaatt agaaaagatg
     6001 aagattgtag atgatgagat tggtgattta gcacagacat ttgaacacat ggcacatgag
     6061 attgacaaaa gtgaaaaatt aaaagaggaa tttatatcat ctgtatctca tgaactaagg
     6121 actcctctta catctataaa ggggtggagt gaaaccttag gttatgaaag tataacaaga
     6181 gaagaactag acttaggttt aggcattata caagatgaaa cagaaagact tataaaacta
     6241 gttgaagaat tattagactt ttcaaggctt tcttcagaca ggataaaact tcatgttgat
     6301 atagttgatg ttgaaggttt aattgtaggt gttgtaaatc aacttaaagt taaagcagct
     6361 gaaaaagata tatcactttt atttgaattt gaaaatgaat ttatagagaa cattcaagga
     6421 gataaaaata gattaagaca ggttctcata aatttaatac aaaattcatt taaatttacg
     6481 agtcaaggtg gttacataaa agttgtagct tctcaagatg aagagataac aaccatatca
     6541 gtagaggata atggttcagg tatagaaaag caaaatttaa ataaagtgtt agataaattt
     6601 ttccaagaag attataataa ggctggtagt ggattaggat tagcaattag taatgaaatt
     6661 gtaaaacttc atggtggaag aatgaaaata gaaagtgaaa agaatgttgg aacaaaaata
     6721 acttttaata taaaaaataa atttgctaaa caagcataaa ttcttagaaa gaggattaag
     6781 atgagaaaca taaaaatgat agtagcatac gatggttcaa gatataaagg atatcaaaaa
     6841 ttaggtgaca ataatatgac tattcaagaa aaacttgaaa atgttttaag taaaatgact
     6901 aatgaaacag tggagataat agggtctgga agaactgata tgggagctca tgcaagagga
     6961 caagtggtaa attttagaac taattgtatg gattctttgg ataaaattca aaaatattta
     7021 tatgaatatt taccagaaga tatagttgta aaaactgttg aagaagttga tgaaagattt
     7081 cattcaagat acaatgtaaa atcaaagact tatatgtata aaattgacaa taataagtat
     7141 cacaatcctt ttataagaaa atatgctact catgtaagta aaaaattaga tttagataga
     7201 atgagaaaag caagtgagta tttagtagga gaacatgatt ttactagttt tgcttcttct
     7261 aaatctaaga aaaaatcaaa tgtaagagaa atttattcta taaatataaa agaagatgat
     7321 aatgttatag aaatatatgt agaaggaaat ggatttttgt acaacatggt aaggataata
     7381 gtaggtgctt tgatagatgt tggtctaaaa agaagagctc cacaagatat taagtatatg
     7441 cttgaatcaa aagatagatg tcaatcatca gatactgcac cagctaaagg actatgtctt
     7501 tggaaagtaa catattagtc aattatataa aactctcttt aaaataaaaa taaaataaat
     7561 tatatagttg acataaatgg aaaaataaac tattatatat tttataagag agatataaaa
     7621 ctttatattt aaatcctgtg agaaagagag taacttctat gtgttttata gagagctgag
     7681 gatggtggaa gctcagcaaa taagtattag tgaaaagaac tttggaggat actaattgaa
     7741 tttagtatgt tagtaggtaa tctcgcctta agagaaagag tggataagtt tatcttatca
     7801 attagagtgg taacgcggat atatttcgtc tcttggtttt tccaagaggc gttttttaat
     7861 atatttaatt attagtgttt atctggtatg ttgaacattt actaattgat tagctaagtt
     7921 tatcaataag agtggtaccg cggatatttc gcctcttagt attttaaaat actaaggggt
     7981 ttttttagta ttaaaatgat tttatgtgag tctaaattct cagagtcatt caatattaag
     8041 aatttctata aaataaaaat tataatttaa gattgggagg aattaaaaat gaaggaaaaa
     8101 gtagtattag catattcagg aggacttgac acatcaatca taataccttg gttaaaggaa
     8161 aattatgaag atatagatgt tatagctgtt tgtggaaatg taggtcagga agataaaatg
     8221 gaagatgtat atgaaaaagc tttacaaagt ggagcatcaa aggcatatgt ggatgatata
     8281 agtgaagaat ttgtaactga aactatattt aaagccgtaa aagcagaggc taaatatgaa
     8341 ggaaaatact tattaggaac ttctttagca agacctataa tagctaaaaa attagttgaa
     8401 gttgctcata aagaaggtgc aaaatatata tgccatggat gtacaggaaa aggtaatgac
     8461 caagttagat ttgaagctac aattgcagct cttgacccga ctataaaagt tattgcacct
     8521 tggagaattt gggatataaa atcaagagaa gatgctatag actatgctga aaaacataat
     8581 ataaaggtaa cagcaactaa agctaaaata tattcagtag atgctaacct ttggcatgtt
     8641 tcaacagaag gtggagatat agagcaccta gaaaatgaac ataaaaaaga tgtttataaa
     8701 caatgtgtag acccagaaga tgcatgtgat gtagctgaat atgtagaagt ttactttgaa
     8761 aaaggtgtac ctaaaaaagt aaatggcgaa gaattatctc cagttgcact gattcataag
     8821 ttaaatgagt tgggttgtaa acatggaatt ggtgttatag atatagttga gaatagactt
     8881 gttggtatga aatcaagagg tatatatgag actccaggag gaacaatcct ttatgaagca
     8941 cataatattt tagaaagtgc tactttagac aaagatacat tacatttcaa acaaatggta
     9001 tcttataaat acggagaact tatttacaat ggactatggt attgtaaatt aagagaatct
     9061 atagatgcat ttatggaaca aactcaagat aatgttacag gaacagtaaa agttaaactt
     9121 tacaaaggaa atataaaacc tgctggaata tttactgaaa atgctcttta tgatgaagga
     9181 atatcttctt ttggaaatag tgaactttat gaccacaaag atgcagaagg atttattaat
     9241 ttattcactt taccattaaa aatcagagct atgaaagctg gaaaataata aaataaatta
     9301 tttaagagtg tataaaaact atttttaggt ttttatatgc tcttttttta tttgaattta
     9361 ttaaattata cagtaaatag tataaaaaaa tatattatag ttccaaatga cttaataatg
     9421 tgaaaatata agacctaaaa tataaatttt ataaatacaa tagttattga ctctctcatt
     9481 gtaagagggt ttatactgaa tctaatgaaa atttatagtg aggagataga ttatatgaga
     9541 aaaataattg aaattaaaca tcattcacat gattatgagt gtatgtggaa tggtatagaa
     9601 gatttatata taaataaaac cggtgaatgc cttccaaacg attttttctt tttactgtca
     9661 ggttttggtt cattttgtta tatgaaaaca aataaagctg atttgaaaag aatggtagct
     9721 ttgggagatg gaaggacaaa acagatgtat aaatttttag caccaattgt tggatttgaa
     9781 tacaaacatc atgagtttaa aaagtatgag caagcattaa agaaagcaaa atctgaaatt
     9841 gatggtggat atccagttat tttaggagct ttagatatgt attatctacc atattataag
     9901 aagctatatc ataaagaaca cattcctttt cattatatat taatggtagg atatgatgat
     9961 gaaagaatat atctgtatga ttgtggtaga acagaattat tacatctttc ttatgataaa
    10021 ttacgtgaaa gcatgaattg caattcccct ggattaagca aagaaaatac tatttgcaca
    10081 ataagaatga atacttcaaa gaataaatat aacattgcta gagaagctat tgctataaag
    10141 cgtgatatgt ttttaaatcc atctaaagga tttttgggat ataaaggttt tgaaaagttt
    10201 attaaagagt tgccacagtg gaacactgaa ttggataaag atgattatga taagattttg
    10261 accaatatgg ttatgttttt tggaacagta ccaacaattc caaatgcaat caaaggaatt
    10321 aaagaaccag atactgttca atttaaaggt ggatttgata aaatgagtag aatgctcaac
    10381 agtattgggg aggaatacaa aaacgactct tttatacaag tagctgatat ttttgataaa
    10441 actgctatta taattgagaa aatatccaat attataatag attatttaac tcaaacatgt
    10501 gatgatacag aacaacttcc gttattgttc tctgaggtct tagagcatat gaaaacagga
    10561 tatttaatct tggatgtata aaaaatatat tgttgaccct aacattacgt aatagtgtaa
    10621 tatgaaaata tagcttgaat atattagtag gtattgatgg ctgtaaaatt aaaatttgag
    10681 gtgtggatat gagaacagtg aaacaagttt ctgatttaac tggaataagt gtgagggcat
    10741 tacattatta tgatgagata ggtcttttaa aaccaaataa aattacagat gcaggatata
    10801 gactttatga tgatgaatct attaaaactc ttcaacaaat tttatttttt aaggagattg
    10861 atattccttt aagagaggtt aaggaaataa tgtcaagcca atattttgat aaggttgaag
    10921 ctctaaaaaa tcaaaaaaaa ttacttattt taaaacgaaa gagactggat gaattgatag
    10981 agcttataaa tcaaactcta agaggagaag gaaatattga ttttaaagag tttgatatga
    11041 gcgagtattt taatgtttta gaagaattta aaagagaaca taggaataag ataattaaaa
    11101 tttatggtag tgtagaaaaa tacaatgaat atatagaaag agttaagtct aatgaagaga
    11161 aaattgctaa aatggctata aaacaatatg gaactattga gaagtttgcc caagctataa
    11221 aaacaaattt tagcagtgat atattgaatt taggagaaaa atttgataga tataaaaatg
    11281 attgtttaaa agaaaaacat ccaaaattaa aagaattata tagaaagctt gtagaagatt
    11341 taagtaagaa tccttcttca acagaccttc aagaaatagc aaaagagata acagatatat
    11401 ctaaaaaaga ttatgaaatt ttctctatgt atactggtga tgacaactgg tattatatga
    11461 ttcaaaatta tctagtaaat cctatgtgga tagaagaagt agataaaaag tatggaagtg
    11521 gagcaggcaa gtttattgga caagtactaa agacttatct aagagataga aaaccaaaga
    11581 taaatacatt gtatgaaaaa cttgtagaag atttgagtag agattgttct tcaagggaag
    11641 ttcaaagcat tgttgaagaa atagacaatg aaatgaaaag aagtaatgaa ttttataaaa
    11701 tagacaatgg tgaaagatac tttgattata tgtcagagct ttatttacaa gattctaact
    11761 atataaaagt aactgataag aattatggtg atggtgcatc taagtttatt ggagaagctt
    11821 ttaagattta ttttgataat aataattgtt gaaaatttgg aagtaaaaaa tatgtaagtt
    11881 ttataaaagc acaaaaataa ggatgtagat tagctccatc tatatcctta tttaaagtta
    11941 ttaaaatata tagtttacat caaacttaac actttcaata atatttccca aatatgtttt
    12001 tatactttca acttcttcta aagattttaa tttattttca gtataaattg ctttgtccag
    12061 aatattaaac cttaatccta tttcatcaaa gtactcatga tttaaaactt tgtaccaatg
    12121 gtttttttct ttattccaac tcgtagatat tgaatcagct tctttttgag ctttttccca
    12181 atcttcattt tctatagatt tttctaaaac agatatattg tctttatatc cttctgtaaa
    12241 atcatatagt ttattgttaa cgtaaaatcc aaataataca aataagatag tccatagtat
    12301 aacaaatgtt gtagatttca atattaatca ccctttttat tttgaataaa tattttgtca
    12361 ttttcatcta aaacacaaat aagaacatct ttaaaagttt ctatgtgatt agattttaaa
    12421 atacccatta accagttagt atctttttga gctatgtcta gatttttatt tattattcta
    12481 ccatccagta taagtgggat aggtaaatga ttaaaagctc ttggaggagt actattgtag
    12541 cttgatgctg gtacaacact tagattacca tcagtctcca aaatagcata ttgtacatct
    12601 tttaggttga agtatccttg aatcctaagt tgttctaata attcatctat actaattctt
    12661 tcttttttaa gctctttata tacaattttt cctttatcta ttaatacaga aggtttacct
    12721 gataaaaatg aacttaattt tctgcttttt aatgataaga atgaaataag agtctgcatg
    12781 attacaagac cagatattgc tgcaacacca tttatgattg gtatattatt attttccatt
    12841 gggacagatg caacttctgc aattaatagt gttataacta aatcaaaaca gttcatttca
    12901 gctatttcac cttttcccat gactctaagt gcaattagca ctgttatgta taatataata
    12961 cttcttatta atactactgt catatctatc aattactcct ttaaaaaata tttacactta
    13021 gttagtatat cctatataaa aaatttaatt caaagtataa aaaaccttaa ataattggat
    13081 tcaactattt aaggtttttt tgataatttt aatattaaat tttttatgga ttttctgtta
    13141 cttttattta ttttaataac agattcaaca aaactctttt tatctcctaa ttttaagata
    13201 aaatcaggat attcttcaaa ttcattgagt tttattaatt tgaaagaaga agatagattt
    13261 ttaaagttaa tgattttaga gttattcact aatacacttc tttttttaaa ataaatttct
    13321 tttttactta tatttagtat agttaatatt tgtttaagat atattattat atcttctgga
    13381 gttctatcaa atttgagttc aaaattttca tcttcaaagt agggaatata atataaaggt
    13441 gttttgagag caggtaattt attatataat tcttcaagaa tatcgatatt tttaggggag
    13501 ttaaagaaaa agcatttaca tacgttcatt aaatccacag catctgaaag tgagttatgt
    13561 ttaatatctc cttctacaga aaaaattttt ttcatattca gtaaagatat ttgcttagat
    13621 aagattctat tattgtatgt aatatgttga gatataaaag gttgaatgtc aatcatcata
    13681 ttaaataact ctaagcattt acctgtatat ttatatactc taaatgaagt tagaaggcca
    13741 gttatatcaa aatcacccca attatatatt gtaacatcat caaatttaca aaaccattta
    13801 taaaaatcat ccataacata taaaagatta ggagcattat tgatatctag ttggttcaat
    13861 ttggttaaat ttttacattt atttgaaagt attttattgt attttggctt aactaaagag
    13921 taataattat ctattgtatt aaattcgtta tcaactaaaa ctccaccaat agaaattaat
    13981 tccgcaccat tatattcttt tggtttattt ttattatcat ctagtatatt aaattcaaag
    14041 tctataaata acttgtattt cataaatcta aagcacatcc tcaggtaaat tatttattga
    14101 gcctaagcat cataagaatc aatagatata atgactctaa acatcaaagg ttcattacaa
    14161 cttttattta tttcataatt taattcaaat aaatctttta agatataatt tataagtatt
    14221 ctttcccaac ccatttttaa attaacttta gagtctatta atatattttc tatcttttta
    14281 aatatttcta tatttgctgg tattatactg agctcaatat tacttttaaa cgggttagac
    14341 tgtaaatctg ttataagtat attttttaat tcctctgtaa gttcagaaat taaactatca
    14401 cctatgtatt tttttatgat ttcctctcta atttctttta aatttagcaa tattagtacc
    14461 tgctacttac atagtagctt ttcctttcct tttaatacta ttattcattt ttgaatctta
    14521 tttagatata ttttaagatt aaaagtcttt agctatataa ttttttaccc atttttaact
    14581 tatattaaca tattacaggc ttatatctcc accctgctta cattgaggat ggagtatatt
    14641 gcctattttg ataaaaatga taggttgtta tttgcctatt cctttttctg ataatttttc
    14701 taacatatct ttggtcattt tatctaaatc ataactaaac ttaaagttcc attcttttac
    14761 tgcactacta ctgtctagag agttaggcca agaatcagct atactttgtc ttacagggtc
    14821 tacatcatat tctatcacaa aatcaggaat atattttttt atagaatcag ctatttcttc
    14881 aggagaaaag ctcatagcag taatattaaa agcatttcta tggatgagtt tatccgctgg
    14941 agcttggatt aaatccacta ttgattgaag tgcatcaggc atatacatca tatccatttt
    15001 tgtaccttct gctatgtaag atttatatct cttatttttt aatgcctcat aatatatatc
    15061 aactgcataa tctgtagttc ctccaccagg aggcgtaaca taagaaataa gacctggaaa
    15121 tctaacacct ctagtgtcta ctccaaattt tgagtgataa taatcacata gtaattctcc
    15181 agaaacttta gtcacaccat atatagtatt aggtctttgt aatgtatctt gaggagtcat
    15241 attttttgga gaattttcac caaaagcagc aattgaacta ggtgtaaaaa atttacaatc
    15301 caattctttg gctatttcaa ggccatttat taatccattc atatttaagt tccaagcttc
    15361 taggggttta ctttcagcaa ctgcagaaag taaagatgct agatgaatta tagtatctac
    15421 atcatatttt tttgcaattt caaaaaaacg attcttatct gtaacatcta atatctcaaa
    15481 tataccagat tcacatactg gattaccttc ttttactctc cttgaactag cgattacatt
    15541 ttgttctcca tactcatttc taagctttat tgttaattct gaacctattt gtcctaatgc
    15601 acctgttata agtatttttt tcaattttac aacctcctaa gggtttaatt tatgtagtac
    15661 tacttatttt tattttagca aatcttttgt ttaaatacaa agaattaaag ataataatat
    15721 acgacttaat tgaatgaagt gcagtaaatt cctaaaaatt taaagttgag atataatggt
    15781 ctattgtata a
//
LOCUS       DAELSY010000093        15759 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.093, whole genome shotgun sequence.
ACCESSION   DAELSY010000093 DAELSY010000000
VERSION     DAELSY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15759
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.093"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(52..1149)
                     /gene="ychF"
                     /locus_tag="KPG09_002535"
     CDS             complement(52..1149)
                     /gene="ychF"
                     /locus_tag="KPG09_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBG0967135.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
     gene            1465..2730
                     /locus_tag="KPG09_002536"
     CDS             1465..2730
                     /locus_tag="KPG09_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG0967136.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            2745..4031
                     /locus_tag="KPG09_002537"
     CDS             2745..4031
                     /locus_tag="KPG09_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG0967137.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            4428..5174
                     /locus_tag="KPG09_002538"
     CDS             4428..5174
                     /locus_tag="KPG09_002538"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBG0967138.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKDDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKHNISEE"
     gene            5204..6139
                     /gene="rsmH"
                     /locus_tag="KPG09_002539"
     CDS             5204..6139
                     /gene="rsmH"
                     /locus_tag="KPG09_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBG0967139.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGKLVDIIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNKGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            6625..6972
                     /locus_tag="KPG09_002540"
     CDS             6625..6972
                     /locus_tag="KPG09_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967140.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIAIFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            6984..8963
                     /locus_tag="KPG09_002541"
     CDS             6984..8963
                     /locus_tag="KPG09_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBG0967141.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            9130..10503
                     /locus_tag="KPG09_002542"
     CDS             9130..10503
                     /locus_tag="KPG09_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBG0967142.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREASAQNAFVA
                     IVGENLDGHTFMKPAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDIYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            10544..11512
                     /locus_tag="KPG09_002543"
     CDS             10544..11512
                     /locus_tag="KPG09_002543"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBG0967143.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            11531..12886
                     /locus_tag="KPG09_002544"
     CDS             11531..12886
                     /locus_tag="KPG09_002544"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBG0967144.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEVVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            12938..14068
                     /gene="spoVE"
                     /locus_tag="KPG09_002545"
     CDS             12938..14068
                     /gene="spoVE"
                     /locus_tag="KPG09_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBG0967145.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            14086..15312
                     /gene="murG"
                     /locus_tag="KPG09_002546"
     CDS             14086..15312
                     /gene="murG"
                     /locus_tag="KPG09_002546"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG0967146.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            15582..>15759
                     /locus_tag="KPG09_002547"
     CDS             15582..>15759
                     /locus_tag="KPG09_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBG0967147.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNI"
BASE COUNT         6206 a         1690 c         2888 g         4975 t
ORIGIN      
        1 tagtttttta gttattatat ataatagtca aagtgtatat gattaatcat tttaaacatt
       61 aaatctaaat aatattacat ctccatcttt aactatgtat tctttacctt ctaatttaac
      121 caaccctttt tctttagcag cagccatagt accatgctca accaaatcat taaaggctat
      181 tgtttctgct ctgataaatc ctctttctat atcagaatgt atttttcctc ctgcctgagg
      241 agctttactt ccaactttta tagtccatgc tctaacttct tttggaccag cagttaagaa
      301 tgaaattaaa ccaagtaaag cataagaaga ttttataagc ttatcaagcc ctgattgttc
      361 aagacctaat gtttctaaaa actctttctt ttcttcagca gaatcaagtt cagaaatttc
      421 tgcttctatt tgtgcacata ctacaacaac ttcggcatct tctgtttctg caaatgcttt
      481 aacttgttga acatatttat tattttctcc attatcagct aaatcatctt ctgatacatt
      541 tgaagcatat attacaggct tagatgtaag taaatccaat gaatttacga atgtttgttc
      601 atcttctgta aattccatag ttcttacaca cttactttct tctaaagtag acattattga
      661 ttttaataaa tcaagttcag aacctaaagt tttatcagcc ttagcagctt tttgagtttt
      721 attaattctt ctttcaagaa tctctatatc tgagaaaatt aattctaagt ttatagtttc
      781 tatatcgcga agtgggtcta cagagccatc tacatgtaca acattttcat cctcaaaaca
      841 tctaacaaca tgaactattg catcaacttc tcttatatgt gataagaatt tatttcctaa
      901 accttctcct ttagaagcac ctctaactaa acctgctata tcacaaaatt ctatagcagt
      961 aggaactatt ttttcagaat tatataattc ttgtagtttg tttaatcttt catctggaac
     1021 agatacaact cccacatttg ggtctattgt acaaaaaggg taatttgcag attctgcacc
     1081 tgcttgagtt attgcgttaa atagtgtgct tttacctaca tttggcaaac caactatacc
     1141 taatttcata ttaatcgtcc tctctcttat tcttttttat attctttata taactttaaa
     1201 caatcaatct ataggattgc aaaattgttt taattataga catacatatt gattatacat
     1261 catatatatc aagcttgtaa actagacttt aaagcatatt gatttaaata atatttgcat
     1321 ttaatttaaa taaaaatatt gcttttagaa aataataaaa aggatataat taaatagaag
     1381 gtttttatat gtgagtatat ataattaaaa aataaataaa tttcaatttt aaaattgatg
     1441 tttaaataaa aataggaggt acatatggtt aatactaaaa aaataataaa tttaggtaat
     1501 aatattaatc ttactttgat aaaaacagaa aagtttaaat cgaatcttat aagtttatat
     1561 gttcagagac tattggatga aaaagaaact acaaaaaatg ctctaatacc atcaataata
     1621 gcaagtggaa gcgctaaata tcctagtgct agagcaatat ccaataaatt agatgattta
     1681 tatggttcat ctatgggagc tgatgctgta aaacgtggag aacgtcaagt attgagtttt
     1741 aaagtaatta acataagtga aaagtattta gatgagagta tatttgaaga agtagtagaa
     1801 ttttttaatg aagtaataaa taatacgctt gttgtagatg gtgggtttaa agaagaatat
     1861 ttaaatattg aaaaagagaa tttaagagaa aagatacaat caataataaa tgataaaaag
     1921 gaatatgcgc aagataaatg tattgaggct atgtgtaaag gagagagata tagtgtaagt
     1981 gaatttggtt atgaagatga aatagatagt ataacttcaa gagagttata tgaacattat
     2041 aaaaacatac ttaaaacatc acctatagat atagttgtag aaggtaattt tgatgaagat
     2101 aaagttgtag atataataag taaaaatcta aaatttgaaa gagaagaaat tataaatata
     2161 ccaagagcag attttataaa aaatgtggat gaggtaaaag ttatagatga acaaatggaa
     2221 ataactcaag gtaagttagt tatgggttat agagcaaatg tggattatgc agacatagat
     2281 aaatattatg ctttagtagt tgggagcaat gtcttaggtg gtggtccaca ctctaaatta
     2341 tttgttaatg taagagaaaa agaaagttta tgttactata tattctcttc aatagagaag
     2401 tataaaagta tcatgtttat atcatctggt atagaaacta aagattatga taaagctgta
     2461 gaattgataa aaaaacaagt agaaagtgta aaagcaggtg atatatctga tgaagaatta
     2521 gaaaacagta aacttgcttt agttaattct atgaaatcta taactgataa tataggtggt
     2581 atgtctgatt ttgttttctc tcagtctatg gcaaagacaa attctgaagt acaagacata
     2641 atatctagta tagaaaaggt tacaaaaaaa gatatagttg aagctataaa aaatatagag
     2701 cttgatactg tatacttctt gagaaattaa tggaggagat gtctatggaa aaaatagtta
     2761 atgatatatt gaaagaagaa gtttattatg agaaattgca aaatggatta gatgtatatt
     2821 ttatgcctaa aaggggtttt atgaaaaagt atgctatttt ggctactaat tatggttcaa
     2881 atgacttaga atttgtacca attggagaag ataagaaaat tagagtgaat gaaggtattg
     2941 cacattttct agagcataag atgtttgagc aacctgatgg aggagatgca tttgataaat
     3001 tttcaaaatt aggagtaaat gcaaatgcat ttactaattt tacaatgaca gcctacttat
     3061 tttctgctac tgaaaatttt tatgagagtt tagaacatct aattgactat gttcaaacac
     3121 cttattttac agatgaaaat gttgaaaagg aaaaaggtat aatagcacaa gagataaaaa
     3181 tgtacaatga tgaccctgat tggaatgtat actttaattg cttgaaggca atgtatgtaa
     3241 attatcctgc tagaattgat atagcaggta ctgttgatag tatatataag ataactaagg
     3301 aagagctata taaatgttat aatactttct ataatccagg aaatatggct ttatttgttg
     3361 ttggtgactt agatgtagaa aaagttatag atgtaactaa aaagtcaaac aattacaaag
     3421 tagacaaact ctctaagagt atagaaagat tttatccaga agaaccagaa agtgtaaaag
     3481 aaaaagaggt tatagaaaaa ttccctatat ctatgcctat gttcaacata ggttttaaag
     3541 atagtaatgt tggtttaaaa gggaaagaac ttttacgtaa agagattgta actgatatac
     3601 ttgtagggat gttatttaaa aaaggaagta agctctatga agatttgtat atgcaaggtt
     3661 taataaatga aaattttgga gctggatttt cgtctcaagt ggattatgct tttagcataa
     3721 tagctggaga ttctaaagag cctaaaaaag taaaagaaat aattcttgat tatatagaaa
     3781 aatcaaaaaa agaaggatta tcaaaagaag agtttgagag aactaaaaag aaaaaaatag
     3841 ggtcatttat taagtgcttt gattctataa actttatagg taatagtttt atatcttatg
     3901 tttttaagga tataaatcta ttggattatt tagatgttat taaagatatt acttttgagg
     3961 aagttgaaga aagattaaaa gagcatttta aagaagagta ttgtgttatt tctattgtag
     4021 aacctaaata gaaacttaat cataataaga ataactaatt aagtattatt tatgttttta
     4081 aatgtttatt ttggtaaaac aaatttataa aatatattta tataaattga ggtaaaaatt
     4141 taagagctat agataaggaa tagaagaggt tgatgtatat atgcatcaat ctttttgatt
     4201 aaaaaatatc tatgtacact tgtaacttta gtcatgagag attcagttta aaaaatttta
     4261 tagtaattat attagtataa tatagtgtag ggatttgtag aataaatttt aatatttttt
     4321 gtagaattaa cattaatgtg ttatcataat ttagtataat tacaattaat tgtaattata
     4381 gcatattgct ttggaaatat tttcaattaa tacaaagagg tgacgttatg gatagagtag
     4441 catttacact gtttggcata gacatcatgt ggtatggaat acttatggca tgtggaatga
     4501 ttttaggaac tttaatagca ataaaagaag caaaaagagt gggaattaaa gatgatgatg
     4561 tattaaatat agctataata gcaattccag ttggactaat ctgtgcaaga atctattatg
     4621 ttgtatttaa ttggagttac tatgctcaaa atatgtctca aatttttaat tttagaggtg
     4681 gtggacttgc tatacatgga gggttaattg gtggaatatt agctggttat atatacacta
     4741 agattaaaaa tattaacttc ttaaaaatgg ctgatacagt tatacttgga atgcctttag
     4801 cacaagctat tggaagatgg ggaaatttta ttaatggaga agcacatgga ggtgctacca
     4861 atttaccttg gggaataatg gttgatggag taaaggtaca tccaactttt ttatatgagt
     4921 ccatatggga ttttggaata tttatagttt tattactatt tagaaaaaat aaaaaatatg
     4981 aaggtcaagt tatagttact tatataactt tatattcaat aggaagattt tttatagagg
     5041 gattaagaac agatagttta atgttaggac cacttagaat ggcacaagtt ataagtctaa
     5101 ttggagttat tggtggcata attgcacatg tttatttatc aaagaaaaat aaacataata
     5161 ttagtgaaga atagtttaaa aagtgaattt aagggaggta actatggaat ttcatcatgt
     5221 atcagttctt ttaaatgagt gtatagagaa tttaaatata aagccagatg gcgtgtatgt
     5281 tgactgtact atgggaggag caggacattc taaagaaata gtaaaaaaac tgtcagataa
     5341 gggattattt atagggtttg accaagataa aaatgctata agcacagcaa aagagagatt
     5401 gagtgaatat gaaagtagag tgaagtttgt tcacagcaat tttgaaaata taaaagagga
     5461 attagaaaaa ataggagttt acaaaattga tggtgtatta gctgatttag gagtttcatc
     5521 tcatcaactt gatgaagctg atagaggatt ttcttatatg caagatgcac cacttgatat
     5581 gagaatggat gttaggtgtg agttttctgc atatgatgtt gtaaatactt atacagaaga
     5641 tgaattaact aagataataa aagattatgg agaagataac tgggctaagc gtatagctaa
     5701 atttatagtt gaagaaagag caaacaaacc aatagaaact acaggaaaat tagtagatat
     5761 aattaaaaaa gctatcccta agaaagctag aatagatgga cctcacccag ctaaaagaac
     5821 ttttcaggca atacgaatag aagtaaataa tgaacttgga gtaattacaa agatgataaa
     5881 tgatgcttct tctataatga ataaaggtgg aagaatatgt ataataacat tccattcatt
     5941 agaagataga atagtgaaaa atgcatttaa gcatttagca tcagattgta tatgtccaca
     6001 acatttacct atatgccaat gtgataaaga atctgaggtg aaaataataa ctagaaaacc
     6061 tatattacct agtgaggaag aaatagaagt aaatccacgt tcaagaagtg ctaagctaag
     6121 agtagcagaa aaaatataag tagttataag gctaaagaat aatcttatat ataaaataag
     6181 attactataa ttaattaaaa agtattatat attttgttga actggtaaag caattaaaaa
     6241 acaattaaca ctataagaaa tgttctttgt tattgagata tataatgaaa atttagatat
     6301 aaaaatatag atatagatgg tattaagtaa tataatggag aacttaggaa tcagagagta
     6361 gatagttagt tacatattat atttatggaa gaaattatct aaaaatacta gaagtgataa
     6421 aaataaatag aaattaatat ctaacctata gattcatata aaagtataag tgaatttata
     6481 ggtttttgtt gtgtatagaa aaaaatacaa ttagattgtg taatagtgta ttaatttagc
     6541 gatgaaattt tagaggaaga aatatgatta ataacaaata tagtatagag gaaaatttaa
     6601 actatatact atcggggtga gaatttgaaa aaaaattata aaatacataa tctaaatgaa
     6661 tataaaaata aaaaaaagaa taattataaa aagagaaata agaaaaaaat aggagctaaa
     6721 tgcattttac tgattgcaat tttttcagta ataatattga atctatgtgg ttattcaaaa
     6781 atctcggaaa tgaagtacga tatctattat ttacaaaaag atttaagaaa aaaagagata
     6841 gtattagagg aactaaaggc aaaagaaact gaaaaaacat caataaaaga aattgagcta
     6901 aaggctaagg aattgctcaa tatggactat ccaaaagatg gacaaataaa gtatatagat
     6961 gtggataact agggggaata gatttgagaa aagtaaagag gataagtaag aaaaggctgg
     7021 tactcgttct tattctagca tgtgcattgt ttttttgcct tgttattagg actgggtacc
     7081 tacaacttat gaagggaaat tggttgagta caaaagcact agaacaacaa acaagagata
     7141 taccaataga acccaaaaga ggaactatat atgatagaaa tatgaaagaa ttagcagtaa
     7201 gtgtaactaa gtatactgtt tggtgtaaac ctgtggaagt agaagataaa aaagaagcag
     7261 ctgaaaaggt agctgagatt ttggatgaag attataaaga tatttacgct ttaataagca
     7321 aaaaaaatat ggctcttgta aaagtaaaaa gatggataga tgatgataag gcaagtcaaa
     7381 taagagatgc taaattaagt ggaatatggg tagcagaaga taatcaaaga tattacccat
     7441 atgggaattt tgcgccatat gtgttaggac atacttcttc agatgcaaca ggtatatctg
     7501 gtgtagaaat gcaatatgat aaaaagttaa aaggaaagcc tggaaaactt atagtaagta
     7561 ctgatgcctc aggaagagaa ataccacaag gaatggaaaa atattatgag cctgtacaag
     7621 gtaatggtct tgtgttgtct atagatgaag taattcaaca ctatactgaa aaagcagtac
     7681 agaaagctta tgagttaaat aatgcaaaaa aggttactgc aatagctatg aaccctaaaa
     7741 caggtgatat acttgcttta gcatcaaaac cagattatga ccctaatgac tcaagaacgc
     7801 caatatatcc atattatcaa gaagaacttg aaaaatataa tgataaagat aaaataaaag
     7861 gctattatca aatgtggaga aatccagcag taagtgatac atatgaacca ggttctacat
     7921 ttaagcttat tacatcttcc agtgctcttg aagagggagt aattaaagat ggagaaaaat
     7981 tcacatgtac aggtagtgta actgttggag gaagaaagat taaatgttgg agacattaca
     8041 gacctcatgg aacacaagaa tttaaacaag cagtacaaaa ctcttgcaac ccagtatttg
     8101 ttgaattagg aagcagatta ggtgttggaa aaatgtatga ttatatagaa tcatttggcc
     8161 ttatggataa gacaggtata gatttaccag gagaagctaa gggaatactg tataacgaaa
     8221 aaaatgtagg accagttgaa ttggctacaa tatcatttgg tcaatctata tcagtaactc
     8281 caatacaact tataacagct atatcatcaa tagctaatgg tggagattta atgcaaccaa
     8341 gagtggtaaa atcttatact gacaataaag gaaatataac tgaaacagta aaaccaaaaa
     8401 aagtaagaag tgttatatct aaagaaacat ctaaaaaaat gttagaaatt gcagaatcag
     8461 ttgtaactga aggtggagga aagatagcat atataccagg gtatagactt ggtggtaaaa
     8521 caggtacagc tcaaaaagtt atagatggta agtatgctcc aggaaagtat atatgttcat
     8581 ttgttggaat agcaccttgt gatgacccgc aaatagtagt tcttgctatt gttgacgaac
     8641 caacaggagt aagtgcattt ggtagtacta cagcaggacc aatagtgaaa gaaataatga
     8701 atgactcttt aaaatatttg ggagttaaac cagtatataa agaagaagaa aaagctgagt
     8761 atgaaaagaa gcaagttaaa gtgccagatg taagaaatct aaaaatagga gatgctgtaa
     8821 aggcattgga ggatgcgaag ttaaaaccag acttagatgc agatatagaa cttccagaag
     8881 atacaaaggt aaaggatata ttcccaaaac ctggagtaaa agttaatgaa gactcaagta
     8941 taaccctata ttttgaaaac taaaaagtaa cagataatta acatataccc ataaacatct
     9001 tcaaaaaaac agaaatatag tatataatta taaagttaag agttatcgta ggcaatgtat
     9061 atataacggt atacattgcc ttttgttgct aagagaatat agctaaggtt ttaaaggagg
     9121 agtttaataa tggagtgctt aacaataaaa gagttagtac ttgcaactaa tgggaagtta
     9181 atatatggag attataatga ctgtgtaagt gatatagtta tagatagcag ggaggcaagt
     9241 gcccaaaatg catttgtagc catagttgga gaaaatttag atgggcatac atttatgaag
     9301 cctgcatatg atagtggatg taaaactttt ataaaaaatg aaagtaatgg aataaaatta
     9361 gaaagttcgg atataaattt aatagaggta aaagatacat ctttggctct aggagatatt
     9421 tcaaaatact ataaagaaaa atttgatatt ccatttatag gtgttacagg tagtgtggga
     9481 aaaactacta ctagagatat gatttactct gccatatctg caaagttaaa tatactaaaa
     9541 aatgaaaaaa acttaaataa tcattttgga gttccattga ctttatttaa tctaaataaa
     9601 gaacatgagt gtgctgtaat agagatgggt atgtctggat tcaatgaaat aaaatattta
     9661 gtggatatag taaatcctaa aattgcagtc atatcgaata taggattatc acatgtagaa
     9721 aaactgggtt cacaagaggg tattttgaaa gcaaagatgg agattacttc aaactttgat
     9781 gaaacaaata ctttaattgt aaatggagat gataagtttt tatctacatt aaaagaaaaa
     9841 gaacatgtat ataagttaaa aacatttggc tttaataaaa acaatgatat ttattgtgaa
     9901 agttatacta tggaagagga tagtttaaca tttatatgtg taataaatgg gaaaaaagag
     9961 gaaatattta taccaacaat aggagaacat aatatatata atgcaatgtc tgctatatta
    10021 gttggtttgt gtctaaatat ttctttagat tatataaaaa aaggtttaaa aaattttaaa
    10081 gggacaaaaa tgagactaga cataataaaa aatgaaaaat taacaataat taatgattca
    10141 tataatgcta gtccagattc tatggatgct gctttaaaga tacttggaag atataaaggt
    10201 agaagggtag cgatacttgg agatatacta gaaatgggtg aaatttcaga gtatggtcat
    10261 agattagttg gaaaatcatc tatgaataat actgatatta taataactat aggtgaaaat
    10321 tcagtgttta ttggagaaga agcaaaacaa ttaggattca attcagccaa tatatatcac
    10381 tttgagaata gagaagatgt gtttaacaag ttaaatgaat tagtaaaaac aggagacact
    10441 attttagtaa aaggctcaag aggcatgaga ttagaaaaaa tagtcgaata tctaaataaa
    10501 taagcttaaa ctaatgagtt aattgacaag ggagaggagt tttatgatgt taggaataac
    10561 agagcttacg tacaccgcat tgattgcgtt tttaatagta attataatag gaccaatatt
    10621 tataccaatg cttagaaaat ttaaatttgg tcagacagtt agagatgatg gaccccaaac
    10681 acatctagct aaaaacggaa cacctactat gggtggaatt ataatgattg ttgcaattct
    10741 aataactgga cttacaagag taaaagttag tcatgatatg gcagtaggtc ttatatgtat
    10801 tgcaggtttt ggttttattg gctttttaga tgattttata aaaataaagc taaaaagatc
    10861 acttggacta aaagcatatc aaaaaataat ccttcaagtt gcattatctt tttatgttgc
    10921 tttttatcag tatacatcat catcaagtgc ttcacaactt atgataccat ttacagattt
    10981 tgtgataaat gttggaatat tatatatacc aataatgatg tttataatcg tagcaatagt
    11041 aaatgctgta aacttgactg atggattaga tggattagca tcaggagtaa cattaatagt
    11101 ttctgtattc tttatgctat ttgcaagttc tattgctgga aatacagaag tcgcagtttt
    11161 agcagcagca actgttggag catgtttagg tttcttagga tttaattcat atccggctag
    11221 agtgtttatg ggcgacacag gttcaatggc attaggaggt gcagttgtag cattttcagt
    11281 tctaactaac tcagtgctta taatacctat aattggagga atttattttg cggaggctct
    11341 atcagttttg attcaagttg gatattttaa ggcaacaaga aagagattct ttaagatggc
    11401 tccaatacac caccactttg aacagtgtgg ttggccagaa acaagagttg tatttatttt
    11461 ttggattata acagttgttt tagcttggat aagtataata gcagtatttt agggctaggg
    11521 ggaatgaaaa atggacttaa aaggaaaaaa agttttatta gtaggacttg caaaaactgg
    11581 tatatctaca attaaacatt tagataaatt gggtgcaagt ataatcgtaa atgatataaa
    11641 agatgaaaat aaattgagaa atatacttga tgaattgaaa tctataaatg acataaaata
    11701 catattagga catcacccag aagatgtaga tgatatagat atggttgtag tgtctccagg
    11761 tgtaccacta gacctaccat tcatattgaa attaaaaaat tcaggcaagt atataattgg
    11821 tgaggtagaa ttagctttta aactatcgaa taatccaatt tttataggga ttacaggtac
    11881 aaatggaaag acaactacaa ctagtttggt aggagaaatt ttttcaagag caaaacgaga
    11941 tacatatgta gttggtaata taggaaatcc tgtaatagat actatagaaa cttcaagtga
    12001 agagtctgtg ttagtaactg aactaagcag ttttcagctt gagagtatag atgaatttag
    12061 acctaaagta agcgcaatat taaatattac agaggaccac ttaaatagac accatactat
    12121 ggaaaaatac atagaagcaa aggcaaatat atttatgaac caaactgttg aggatttctg
    12181 tgtgttaaac tatgatgatg aggtagtaaa atctttagct gataaatgca atgccaaagt
    12241 aatttacttt tcaagaacta aaaaagtaaa tggaggagta tatttagaaa ataatgacat
    12301 aataatagat atagatgata aaatcaaatt tctaaataaa gatgatgtaa gtttacctgg
    12361 aggtcataac ttagaaaatt gtatggcagc tatagcaata gcatatgtct gcaaaattga
    12421 tttagaagtt ataaaggatg tacttatgac atttaaagga gtggaacaca gacaagagtt
    12481 tgtaagaaat ttagacaatg taatatatgt aaatgattca aagggaacta atccagattc
    12541 cactataaaa gctatacagt cttatgatag acctataata cttatagcag gggggatgga
    12601 taagggaagt aattttgatg aacttcttga aactgcaaag tcatatgtta aatcattggt
    12661 cttattagga gaaactgcat caaatataga aaattgtgct aaaaataaag gatttaatga
    12721 catacatata gtaaaagata tggaagaagc agttaagact tcttatgaaa tttcaaaaag
    12781 tggagatata gtgcttcttt caccagcttg tgcaagttgg gatatgtatg aaagttttga
    12841 agtaagagga aaagacttca aagataatgt aaacaattta aaataatctc ttacctctaa
    12901 acttacttaa atggaagaat ggaggtagat tagttggatg cctaaagaaa atttgaaaaa
    12961 acaaattgac ataaggatga agactgaatt tgatggagta gtgttttaca ctactatgct
    13021 tttagtattt gtaggaatag ttatggtgtt tagtgcaagt tttatacaat cttcatttaa
    13081 acacaatgat gcgtactatt ttctaaaaaa gaatgtaata tatgctgtac ttggatttat
    13141 agttatgatt attacatcaa gaatagatta tagtttttgg aagaaaaatg ctacagcaat
    13201 tggagctata gcagtagtat tattattgtt agttttgacc cctttaggga tagaagcaaa
    13261 cggagcaaaa agatggttgg gaataggagc tttaacattt caacctgcag aaatcgcaaa
    13321 gtttgctaca ataatattaa ctgcaaaatt aattgaaaaa aattatgata aaataaaatc
    13381 tttgacaaaa ggtgttgtac cacttctcgt agttccaggg atattttttg cgttgataat
    13441 acttcagcca aatttgtcaa cagcaggtac tgtaattcta gttacttttg taatgatatt
    13501 tgttgctgga atggatatga aaattgtatt tgctatgata gggagtgggg ctgctttatt
    13561 tgctgcatta gtaattgctg aaccctatag acttagtcgt gttacatcat ttttagatcc
    13621 ttttcaagac cctcttggta aggggtatca ggtaatacaa ggtctatatg cactaggttc
    13681 aggtggattg tttggtctag gcttaggaaa gagtaaacaa aagtattttt atataccaga
    13741 gccacaaaat gactttatat ttgctataat aggagaagag ttaggattga ttggttgtat
    13801 aatagttatt atgctatttg tagttttagt ctacagatgt gtaagaatag ctttgaagac
    13861 ttctaatgta tttgcttgta tggttgttat aggtataggt gcacaaattg gaatacaagc
    13921 agctttaaat atagcagttg cgacatcatc aatgccagct acaggtgtag cactaccatt
    13981 tataagttat ggaggaactt cactcaccat ctttatggga gctgtaggaa tagtgttaaa
    14041 catctcaaag catgtcaaaa ttaattaaca ggagatgatg atttgatgaa agttttatta
    14101 tcaggcggtg gaactggagg gcacgtgtat ccagctatag ctattgcaaa caaaataaga
    14161 gatgagcatc cagacgctga gataattttt gttgggacgg aaaagggaat agagtcagaa
    14221 atagttccta agtatggatt tgaattaaag actgtaacag ttcaaggttt taagcgaaaa
    14281 atagatttcg ataatgtaaa aagagttttt aagcttttta aaggtttgga gcaatcaaga
    14341 aagattgtta agaaatttaa accagatata gttataggaa ctggtggata tgttagtgga
    14401 cctgttttat ttaatgcatc tatggggaaa ataccagcca taatacatga gcaaaattct
    14461 tttcctggtg taactaataa gattttatct aaaacagtta caaaggtact tacaagtttt
    14521 gaagattcgc ataaaagatt tccagaggca gcagaagata aattagtttt tacaggaaat
    14581 ccagttagaa aagaaatttt attatcaaga aaaaatattg caagaaagaa tctatcaata
    14641 agtgacgaaa aaagaatggt actttgctat ggtggtagtg gaggttcaag aaaaataaat
    14701 gatgcaatga gacttgtaat aaagaatatg gtaaatgaag atatagcttt tatatttgct
    14761 acaggcaaaa gctattatga tgaatttatg ggaagtatta gcgatataaa tcttaaacca
    14821 taccaaaaag ttgtgcctta cttagaagat atggcaaatg cacttgcagc aagtgatttg
    14881 gttataggta gcgcaggagc aatttcatta gctgagataa cggctcttgg aaagccatct
    14941 ataataatac ctaaagctta tactgctgaa aatcatcaag agtataatgc caagagtata
    15001 gaaaaacaag gggcaggaat tgctatttta gaaaaaaatc taactccaga gagcctaaat
    15061 acagcagtat ttaaactttt aggagataga gaacttttag ttgatatggc aaatgcaagt
    15121 aaaactatag gtaagcctga agccatagac cttatatatg atgagataat gaaagtatac
    15181 aattcaacac aaaagtcaac ttctaaaaaa acaaaaaagg aaaaagttat taaggaagta
    15241 aaagaaataa aaaaagaaac gactccaagt atagaaggtc aagctaaggt aataggaata
    15301 aagaaaagat aagtaaatat ttacccctaa tttataatat attagatttt ctaaattatt
    15361 ataaattagg ggtttatttg tatgtataca gatttaaata tataaatagt atgtagtata
    15421 ctatgtttat tttgatgaaa tattgattta tttataaaat ttaatacctt atttggatgt
    15481 tatgtaataa aatgcaaaaa tttgacttta aatattataa tttttttgta ggaaaaacaa
    15541 gctctaaagg tgtatttatt aaccaaaggg gttgattata tttgaaaaaa agaagaaagt
    15601 taaatacgaa taatgtcatg atagttttag tttttatact tatgttatct ttttgtatat
    15661 gtataattgt aaaaagtgat ttatttgatg taaagaaaat agatgtaatt ggaaataaga
    15721 gggtgaccaa atcaaatata atgaaagaac taaatataa
//
LOCUS       DAELSY010000094        15396 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.094, whole genome shotgun sequence.
ACCESSION   DAELSY010000094 DAELSY010000000
VERSION     DAELSY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15396
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.094"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            117..572
                     /locus_tag="KPG09_002548"
     CDS             117..572
                     /locus_tag="KPG09_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG0967148.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            621..1205
                     /locus_tag="KPG09_002549"
     CDS             621..1205
                     /locus_tag="KPG09_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBG0967149.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSREQMDEVKLAMGFKL"
     gene            complement(1210..1533)
                     /locus_tag="KPG09_002550"
     CDS             complement(1210..1533)
                     /locus_tag="KPG09_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0967150.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLESL"
     gene            1685..2203
                     /locus_tag="KPG09_002551"
     CDS             1685..2203
                     /locus_tag="KPG09_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0967151.1"
                     /translation="MNFVELTKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGRQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(2379..3047)
                     /locus_tag="KPG09_002552"
     CDS             complement(2379..3047)
                     /locus_tag="KPG09_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967152.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            3162..4577
                     /locus_tag="KPG09_002553"
     CDS             3162..4577
                     /locus_tag="KPG09_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0967153.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNSKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQYVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDHEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            4800..5258
                     /locus_tag="KPG09_002554"
     CDS             4800..5258
                     /locus_tag="KPG09_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0967154.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            5255..5839
                     /gene="clpP"
                     /locus_tag="KPG09_002555"
     CDS             5255..5839
                     /gene="clpP"
                     /locus_tag="KPG09_002555"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG0967155.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            6161..7237
                     /locus_tag="KPG09_002556"
     CDS             6161..7237
                     /locus_tag="KPG09_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0967156.1"
                     /translation="MIAISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            7304..9343
                     /gene="bclA3"
                     /locus_tag="KPG09_002557"
     CDS             7304..9343
                     /gene="bclA3"
                     /locus_tag="KPG09_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HBG0967157.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGGTGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGGTGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGATGATGI
                     GITGATGLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGVTGADGATGATGATG
                     ATGPTGADGLVGPTGATGATGADGLVGPTGPTGATGVGITGATGATGATGPTGADGLV
                     GPTGATGATGADGVAGPTGATGATGNTGADGATGPTGATGPTGADGLVGPTGATGATG
                     LAGATGATGPIGATGPTGADGATGATGATGPTGADGLVGPTGATGATGATGPTGPTGA
                     SAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAF
                     SMPRDGTITSISAYFSTTAALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPL
                     TGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            9512..10273
                     /locus_tag="KPG09_002558"
     CDS             9512..10273
                     /locus_tag="KPG09_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG0967158.1"
                     /translation="MKNKIETSMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            10582..10860
                     /locus_tag="KPG09_002559"
     CDS             10582..10860
                     /locus_tag="KPG09_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967159.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGEYIYIPRKSNNKKEWGSSTS
                     TREDLSIRNMQIYEDYQVGYDLKYLSEKYYLSLKSIQRIVLQEKRKDS"
     gene            11153..11728
                     /locus_tag="KPG09_002560"
     CDS             11153..11728
                     /locus_tag="KPG09_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBG0967160.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPETLEQFHTNDYNLAKQYAMNTELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            12166..12876
                     /locus_tag="KPG09_002561"
     CDS             12166..12876
                     /locus_tag="KPG09_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0967161.1"
                     /translation="MISIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSSGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            12870..14186
                     /locus_tag="KPG09_002562"
     CDS             12870..14186
                     /locus_tag="KPG09_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0967162.1"
                     /translation="MLTNLSIGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIFIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMI
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYIDNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIENND
                     ICSIFANSLDNAIEACMDINNELEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQ
                     TSKDDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            14198..14338
                     /locus_tag="KPG09_002563"
     CDS             14198..14338
                     /locus_tag="KPG09_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG0967163.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            14397..14978
                     /locus_tag="KPG09_002564"
     CDS             14397..14978
                     /locus_tag="KPG09_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG0967164.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNISVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNNDQ"
     gene            15055..>15396
                     /locus_tag="KPG09_002565"
     CDS             15055..>15396
                     /locus_tag="KPG09_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967165.1"
                     /translation="MNLLNIVESIVSCIKGLARRGINMSSIKVISDFITKIIRKNILF
                     AITVIIINYHLIQRWTHISNRGISFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTV
                     KILCRNNFDYFL"
BASE COUNT         6024 a         1788 c         2689 g         4895 t
ORIGIN      
        1 aaattttaag gaaaaataac atatttgtat taaattttat tgaaatgatt aaatttattt
       61 acttaatgta aattattagc taaaattgta ttaaataatt aaggaggtga gtagctctgg
      121 atgaaataga tttaaaaata attaattctc taaaaaagaa tagtagggct tcaacctctg
      181 aaataagcag acaaattaat ttatcagtac cagcagtctc agagcgtata aggaaaattg
      241 agcagtcaaa tttaattcaa aagtacacta ttcaaattaa tcgggaaaaa acaaaatata
      301 agcttttggc ttttatattt atcaatactg atttatctaa aagtatgaaa gaatttaaga
      361 aaactatatt ggaatatgat actgtattag aatgtcatca tatttcagga gagtatgatt
      421 acttaataaa agtattagca gaagatacaa gtaatcttga agagtttata tcagtgactt
      481 tgaaggaaat tggggggatt caaaaagcta atacaaccat tgttttatct acaattaaag
      541 aagaatttaa tacaataaaa gatatttttt aaataattta ctagtaaagt gttaattata
      601 ttttaggagg aaaatagaat atgaaagttt tagttttttt agcaaaaggt tttgaaacta
      661 tggaatttag tgtatttgta gatgttatgg gatgggctcg taacgattat gggcatgata
      721 ttgatgtagt gacttgtgga tttaaaaaac aagttatgag tacatttaat atacaagtat
      781 tagttgataa gacgatagaa gaagtatgtg ttgatgatta tgatgcccta gctattcctg
      841 gaggatttga agaatttgga ttttatgatg aagcatatga ttcatctttt ttaaatctaa
      901 taagggagtt taattcaaaa gaaaaaataa tcgcttctat ttgtgtggca gcattgccag
      961 ttggaaaaag tggtgtttta aaaaatcgta aagctactac atatcatttg aaaaatggta
     1021 aaagacaaag acagttaagt gagtttgatg taaatgtagt taatgaacca attgttgtag
     1081 ataaaaatat tataacctca tattgtccag aaacagctcc acatgtagct tttaagcttt
     1141 tagaaatgtt gacatctaga gaacaaatgg atgaagttaa attagcaatg ggatttaaac
     1201 tgtaatgctc tatagacttt ctaagtaatc agaaccccag tcacacatgg aatctaaaat
     1261 tggaataata ctctttccaa aatcagatag cgagtattct acttttggag gaacaacagg
     1321 ataaaccact cttataatta aaccatctga ttctaattca cgtaattgtt gagttaacat
     1381 cttaggcgtt gctttaggaa ttaatttttt taattcatta aacctcaata tagtgtcttt
     1441 aagatgccaa agtataagtg tcttatattt accaccaatc aatgcaagtg ttgcttctat
     1501 tgggcaactg taattatcta aacatttttt catttttgag ctcctttatt tattttaaag
     1561 tatcttttta gatactatat tacaaaaaag tgcattattg taatatagta ccatagtgat
     1621 aaaattatag cataggttaa ataaatcatc aacttagcct aataaaacaa tagggggtaa
     1681 ataaatgaat ttcgttgaac ttacaaaaaa aagatattct tgccgtaatt atcaagatag
     1741 aaaggttgaa aaagaaaaac ttgagaaggt tttagatgtt gcaagaatag ctccaactgg
     1801 tggtaatcgt caacctcaaa gattgattgt tattcaggaa aaagaaggta taaataaact
     1861 ttctaaggct gccaatatat atgatgctcc attagcaatt cttgtatgtg gagataaaga
     1921 caaagtttgg acaagacctt ttgatgggag acaacttaca gatattgaca caagtatagt
     1981 tacagaccat atgatgttac aggcaactga acttggattg gcaagtgttt gggtgtgcta
     2041 ttttaatcca gatataatta gagaagagtt tagtttgcca gataatctag aaccaataaa
     2101 tatattattg atgggatatg aatctaagat accagagagc ccagagagac atgaaaaaac
     2161 tcgtgttcct ttaagtgaaa ttgtttctta tgaaactctt taattataaa attaagttat
     2221 tcataggata aatgtaataa agtgtttatt ataaacaaaa ttcaaaataa ataatatcta
     2281 catataatta attttagtga agtatatcta gtttaactag aatgtatact tcactaattt
     2341 atttattcaa aatatttatg aggattttaa tatattaact aaaatatatc aattgaatta
     2401 acttttaaat ctggaaaagc acttgaaata tagtgtttta tgtcatttaa atggtcttga
     2461 atttgctttt ctgatatttt atcaggtcca tatacaaaat acataatatt tttagtatct
     2521 tttgttatag ctgttcgatt atcagggcca ttaagaatac tagataatat accaatatta
     2581 tctgataaaa ataaatcatc ttttgttaaa atatgctctt taccacctat accataaaaa
     2641 tgttcacttc catttgctaa ttctattttc aaaggtaatt ccattttatc aaggtcatga
     2701 cctgctgtta gaagaagatt tttcaattct gctaaaaaca ttgactcaat tgttacacct
     2761 acatctggga taccttttga ttttaataga atagattcta attgaagcaa tacagggtaa
     2821 gttttcttga attttttata atactttata taacttgaaa caggctctgt tttaattttt
     2881 tctttacgat tgtattctgg gtgttgttct ataatatttt taattgtaga gtttttaaga
     2941 actaaaaaat tttctttatt aggttttgaa tgtgtaatat ttataaccat aactccaaat
     3001 ttaatattgg gataaatttc tttaacttta tgtgatattt ctaacataat atagactcct
     3061 ttttatttta tattttatga tataatctta gcaagttaaa agtggatttt gtagttccat
     3121 ttttatagta attttataga accacttttg gaggtattag aatgtggatt acgattaata
     3181 gaaatagtaa tatatcatta tcaagacaaa tttattgtga gataaaagat atgattctgg
     3241 atggaagctt aaagtttgag caaaaactgc catcatctag gactttatca aaagaactat
     3301 ctgtatctcg taatacagtt ttagatgcat ataatcaatt aattgcagaa ggatatttgg
     3361 aaacaaggaa tggatatgga acaattgtag ctaaaggaat atcagggcat aaagttgatt
     3421 ttaagttaaa tgatactttt agagatgaaa aagatttaca tactaataaa caaaaaaatt
     3481 atatagattt tcgttcagga gttccagaat taaaactatt tccacgcaaa gaactttcaa
     3541 agctatataa acaaatactg tatgatttat cagattcgga tttcagatat ggaagtacag
     3601 ctggtgtatg ggttttaaga gtggaaatat ctaagtattt atttcgtact agaggaatta
     3661 gatgtaattc caaaaacatt atgattgttt ctggttcaac acaaggttta tctttaattt
     3721 cacagctttt atacaaaagt aataaaaagg ttgctgtaga agaccctatt cataaaggct
     3781 tattaaaggt tatttcatct attggatatt cagttgttgg aattaaagta gatgataagg
     3841 gaatgaatac caacttaata aaatcaataa aggggatttc ttttgtttat actacacctt
     3901 ctcatcaata tccattagga gggattttac ctattcaaag aagactagag cttattaaat
     3961 gtgcaacaga gaatgattac tatataattg aagatgatta tgatagtgaa tttagatatg
     4021 agggtcaacc aattagttct ctttatgaac ttagcccaaa taaagtaatt tacataggtt
     4081 cttttagtaa aatattatct ccagctattc gtgttggatt tatgttatta ccagacaata
     4141 ttttagaaag ctataaaacc ttaaaaatgt actctgatgt tcatacagaa acaatattgc
     4201 aatatgtatt agctgaattt attggaaatg gaggtttaga gaagtacata tttagaatga
     4261 agaagttgta caatagaaaa cgtaaatatc tcataaaagt tttaaacacg gaatttaaaa
     4321 atgaatttaa aataaaaggt gaagcagcag ggcttcatat agttgctcat ttctataatg
     4381 ttaaatttac ggaaagttta attcataaaa tttttgaagc aggtataaaa gtttatccta
     4441 tacaacaata tgcaattgag aatatattag aatatgacca tgaaattata ctaggttatg
     4501 ctcatcttag ttttgatgaa attgaaaaag gtgtaaaaat actcagcaaa ataatacata
     4561 tgaatcaagg tacatagata aaacatgtag caatctacat ataatagatg taattaagtt
     4621 taaactttaa aagaaatata tattaatatt tttgtataaa aatacttgat attgttttat
     4681 cgatacttta ttgctataca ctaaattaag atagcaattt atattaagaa aaaacttttt
     4741 gcataggtta taatacttaa aaggagagga tagagttgaa aacaaataca gatgttatta
     4801 tggagataat tgattatata gaagaaaatc ttacagaaaa tctaaattta gataaaatag
     4861 cttatattgc taattattca aaataccatt tacacagaat gtttacaaat gttgtaggat
     4921 gtactatcca tcaatacata cagaggagga gacttactga agcagctaga caacttatat
     4981 ttacagacaa atatattata gatatagcta ttacagtagg atattctaca catcaatctt
     5041 ttacactagc atttagaaat ttatataaag aatctccaca agtatataga aataaagatg
     5101 aattttatcc tattcaacag agatttgttt taaatctaaa tcatcaaatt caaagcgaaa
     5161 aatgtatttc ttatgcaaat atgaattata aacaaagaag agatgaaatc aattcttatc
     5221 ttaactttaa tattaattgt gaggtgaaag tagcatgagt ttagtgccac atgtaattga
     5281 acaaacaggt caaggggaaa ggtcttatga tatatattca aaattattaa aagatagaat
     5341 tatatttata ggagaagaaa ttaatgatgc tattgcaagt ttagtagtag ctcaattact
     5401 gtttttagaa tcagaagacc cagattcaga tataattata tatataaata gccctggtgg
     5461 ctctgctaca tcaggatttg ctatatatga tactatgaat tatgtcagat gtgatgtatc
     5521 gactatctgt attggtatgg ctgcaagtat gagtgctttt ttacttgcag gaggaacaca
     5581 tggaaaaaga tgtgctttac ccaattcaga gattatgatt catcagccaa tgggaggagc
     5641 taaaggtcaa gcaacagatg taaaaatagc tgtagataat atattgaaaa taaaagaacg
     5701 attagataaa attctttctg aaaatacagg taaatcaata gaagatatta gaagagatac
     5761 agatagagat aattttatga ctgcactaga ggctaaagag tacgggctga ttgattatat
     5821 aatgaatagg aatgaatagg aatgaataaa gtatcaaagt atcatattaa aatgtctgtt
     5881 ttacaaatat atatataata ttaaataaga gtgtctcaaa atgaactttt tagtttatgg
     5941 gacactcttt tttgtatgaa attaaatatg gttttatcat ttcaacaatt attaccaaat
     6001 ttcaagttta ataaattcta ctataaaaaa tacataaaag ataaggttct ttagtaactt
     6061 tctgtcgtca aaagttttta taaatttaat aacaaaacaa gagaaaatac catatattac
     6121 ttatgtctgc aatatataga agacaaagga gttgtacatt atgattgcaa taagtttgtg
     6181 catgattgtt aagaatgagg aagaagttat aggcagatgc ttagaatgtg taaaggatat
     6241 agtagacgaa attataattg tagataccgg ttctacagat tccacaaaag aaatagtctc
     6301 aaaatataca gacatggtgt acgatttcga atggatagat gatttctctg ctgcaagaaa
     6361 tttttcattt tctaaagctt ctaaagacta tactatgtgg cttgatgcag atgacataat
     6421 tttagaagct gatagagaaa aattattaaa gattaaagaa agcttggata cttccattga
     6481 tattgttatg gcaaaataca atgtatcctt tgatgaaaat ggtaacccaa ctctctcata
     6541 ttatagagaa cgattgttta aaagaagttc taatcataaa tgggttgacc ctattcatga
     6601 agtagtacct ctttttggag aggtatttta ttctgacatt gcaatatctc ataaaaagct
     6661 tcatagacag gaccctctaa gaaatcttag aatttttgaa aaaatgattt cagaaggaaa
     6721 aacattggag ccaagacacc aattctatta ctctagagag ttgtactata atgctaggta
     6781 taaagaagca atagaaggtt ttactaagtt tttagactca tcaagaggat ggattgagga
     6841 ttgtatcagt gcctgcagag atttagccac atgttattac ttaattaatg atgaaaaaag
     6901 tgccttatat tcattgttta gaagtttcga atttgatgag ccaagagcag aaatttgttg
     6961 tgacatagga aagcatatgt ttgatagaca aaaatataaa gaagccattt tttggtataa
     7021 ggtagcatta acaagagata agaatgatac taatggaggt tttaagtcaa atgattgtta
     7081 tggatatata ccatctatac aattaagtgt ctgttatgac aggttaggag aatcagataa
     7141 agctatttat tatcatgaaa aaacaaaaga aatcaaacct aacgatagtg ctgttttgca
     7201 caatgaaaat tattttagta aatttaaaaa tagttgatag gtcacaatca tactaaattt
     7261 gacatattat atatagagct taaaagctca aatatatcag gaggtgcttt taataatgag
     7321 tagaaataaa tattttggac catttgatga taatgattac aacaatggct atgataaata
     7381 tgatgattgt aataatggtc gtgatgatta taatagctgt gattgccatc attgctgtcc
     7441 accatcatgt gtaggtccaa caggcccaat gggtccaaga ggtagaaccg gcccaacagg
     7501 accaacgggt ccaacaggtc caggagtagg gggaacaggc ccaacaggac caaccggtcc
     7561 gactggccca acaggaaata cagggaatac aggagcaaca ggattaagag gtccaacagg
     7621 agcaacaggg ggaacaggcc caacaggagc gacaggagct atagggtttg gagtaacagg
     7681 cccaacaggc ccaacaggag cgacaggagc aacaggagca gatggagtaa caggtccaac
     7741 aggtccaacg ggagcaacag gagcagatgg aataacaggt ccaacaggag caacaggggc
     7801 aacaggattt ggagtaacag gtccaacagg cccaacagga gcaacaggag taggagtaac
     7861 aggagcaaca ggattaatag gtccaacagg agcgacagga acacctggag caacaggtcc
     7921 aacaggggca ataggagcaa caggaatagg aataacaggt ccaacaggag caacaggagc
     7981 aacaggggca gatggagcaa caggagtaac aggcccaaca ggcccaacag gggcaacagg
     8041 agcagatgga gtaacaggcc caacaggagc aacaggagca acaggaatag gaataacagg
     8101 cccaacaggg gcaacaggag caacaggaat aggaataaca ggagcaacag ggttaatagg
     8161 tccaaccgga gcaaccggag caaccggagc aacaggccca acaggagtaa caggggcaac
     8221 aggagcagca ggactaatag gaccaaccgg ggcaacagga gtaaccggag cagatggagc
     8281 aacaggagcg acaggggcaa ccggagcaac aggtccaaca ggagcagatg gattagtagg
     8341 tccaacagga gcaacagggg caacaggagc agatggatta gtaggcccaa caggtccaac
     8401 aggggcaacc ggagtaggaa taactggagc aaccggagca acaggagcga caggtccaac
     8461 aggagcagat ggattagtag gtccaaccgg agcgacggga gcaacaggag cagatggagt
     8521 agcaggtcca accggagcaa caggggcaac aggaaataca ggagcagatg gagcaacagg
     8581 tccaacaggg gcaacaggtc caacaggagc agacggatta gtaggtccaa caggagcaac
     8641 cggagcaaca ggattagcag gagcaaccgg agcaacaggc ccaataggag caacaggtcc
     8701 aacaggagca gatggagcaa caggggcaac cggagcaaca ggtccaacag gggcagatgg
     8761 attagtaggt ccaaccggag caacgggagc aacaggggca acaggtccaa caggcccaac
     8821 aggtgctagt gcaataatac cttttgcatc aggtatacca ctatcactta caactatagc
     8881 tggaggatta gtaggtacac caggttttgt tggctttggt agttcagctc caggattaag
     8941 tatagttggt ggagtaatag accttacaaa cgcagcaggg acattgacta actttgcatt
     9001 ttcaatgcca agagatggaa caataacatc tatttcagca tacttcagta caacagcagc
     9061 actttcactt gttggttcaa caattacaat tacagcaaca ctttaccaat ctactgcacc
     9121 aaataactca tttacagctg taccaggagc gacagttaca ctagctccac cacttacagg
     9181 tatattatca gttggttcaa tttctagtgg aattgtaaca ggattaaata tagcagcaac
     9241 agcagaaact cgattcttac tagtatttac tgcaacagct tcaggtcttt cattagttaa
     9301 tactgtagca ggatatgcaa gtgcaggaat tgcaataaat tagattagat aagaaaaggc
     9361 ttctaatatc gtattgatat taggagcttt tttaagaatg attgatgaac atagttttaa
     9421 tattaattag tgcgcatatt ataaaatact attatataat ataaaataat aacattaaat
     9481 tggaaatata gaattatcta ggggtgatac tatgaaaaat aagattgaaa catcaatgaa
     9541 caactttgta aaaaattatc ataaatcaaa taatataact acaaagtggc aagaactttt
     9601 gtttgcttat gcatctgctg aagatgatat gtttcagaag ctaaaaagtg ttgtaggaac
     9661 atctcatgct atgccaaaag attttctgga aaatggaaaa actataatta catattttat
     9721 accttttgat gaaagtgtaa ataatagtaa tattggaggg tttgaatgtt ctgaaatatg
     9781 ggcaagagcg tacatagaaa ctaatatgtt gatttcaaat ttaaatgatt tcttatgtga
     9841 agaactagag aagatggggg ctaattcata tgctattcct gggtcgtata attttgacag
     9901 tgaaaaattg gtaagtgact ggtcacaaag acatgtagca ttcatagcag gtcttggaac
     9961 atttggttta aataatatgc ttattacaga aaaagggtgt tgtggaagaa taggaagcat
    10021 aatcacaagt ttagaattag agccaactaa aagaccagaa aatgaatact gcttgtataa
    10081 acataataat agttgcaaaa aatgtgtcaa caaatgcgtg aaagaagctt taaaagaaga
    10141 tggatttaat aggaataagt gttatgaaat gtgtatgata aatgaaaaaa aatacactta
    10201 tataggtggt tgtgatattt gtggaaagtg tatagttggg gttccttgtt cggtaataaa
    10261 tccctcaaga taaaggactt atgttgatta ataactttaa atatatctaa taagaagaaa
    10321 aaagttagtc tctaaaatag atgaattatg aaaataaata aagataatat aaaggttaga
    10381 tgaagaagtt atgttaattg acaactattt atactgctga tagacttata tgtatcgttg
    10441 caatatataa ggaggggtga gatttatgtt tgatttaatc tttatttttg catagcaagg
    10501 gctattgcaa atataaaatt taatatataa atagcccttt gctattccta ataaaaaatt
    10561 attatttaat aggagtgcag aatgagctat aaaaaagcaa tacatatatt accagaagaa
    10621 ttacttgaat tgatacagga atatgtagat ggagaatata tctatattcc tagaaagtct
    10681 aataataaaa aagaatgggg aagtagtacg tctactcgtg aagatttatc aattagaaat
    10741 atgcaaattt atgaagatta tcaagttgga tatgatttga aatatttatc agaaaaatat
    10801 tacttgtctt tgaaaagtat tcaaagaatt gtattacaag aaaaaagaaa agatagttaa
    10861 gaatgagcca gtatagtttt ataattatac tggctcattt tcttttgata aaagtgtttg
    10921 aggtatagtt tcttataagt ggatgctata atttaaataa tttcaataac ttgtacagca
    10981 gataacgaat agagttgtaa agtatctaat aagaagtagt agctttttat atgtttgaaa
    11041 ttaaaattta ttaaaattag gaggtcttgt tttgaaaaaa atacttgatt atattccacc
    11101 ttttcataac tttctattaa tttagtaatc ttatagaaag gaagttgcat ttatgaaaaa
    11161 aattacttgg gaggtggaat atatctccca gttacctgta attaggtatt gtaagaaatg
    11221 tggaaaaaag actgaatata tttgttcagg tttatttagg gttaatgctc aacataaata
    11281 tttggatata tggttaatat acaagtgttc aaattgtgat tcaacatgga actcaactat
    11341 atattctcgt gtcaatccaa aaagcttaaa tccagaaaca ctagaacaat tccatacaaa
    11401 cgattataat cttgctaagc aatatgctat gaatacggaa cttttacata gaaatggagc
    11461 agaagttggt attccagagt ataaaatagt aggacaagaa attaatatta atgaccttac
    11521 acaagttcat attattagta aatatccatg tcagttaaag gtatcaacat tattacgtga
    11581 gaaactaggt ttatctagga aggtatttga cgaaatgttg gattgtggga ttattagaag
    11641 taactcagga gttgatttaa agaaaggtaa attaaattat gaaattatgt tagatatagg
    11701 caaggagaag caaaagtctt caaattgatt agaataacta acttaatata ttttcaggaa
    11761 catcatttaa aaaaatagtg gctttaaaaa atttaaagcc actatttttt aatatgtttt
    11821 atatgaatat ttaaaatata gtgtgtattc ttcaaaatta tagcgataat taaaatatta
    11881 ctataaaaag gaaggtgcat actttttgtt taacaaaaac tagatactaa aaacttctcg
    11941 aagaatatgt tattaaaatc gctaaatata ttcaatatat attaaaaaat tgtattataa
    12001 tgaaaattaa aatagattgt aaatccaaaa agtaatactt aatgtccaaa aagctaaaat
    12061 taaaatctaa atagctattt gctacagaag tatgttaaaa tatgtaaaat gaatagaaat
    12121 tttatatatt tattaaaaat atataagaaa ggatgtttaa gaattgtgat tagtatagga
    12181 atatgtgatg atgaactaca ttatcgaata aaaataaaag atatattaag tgaaatatta
    12241 agttcatatc ctataaatta taacatatat gaattttcat caggagaaga gttactaaat
    12301 aactatccta aagatttaga catattaatt atggacatac aaatgaaaac aataaatggt
    12361 atggatactg ctaggaagat aagagagttt gatcataagt tagaaataat atttgttact
    12421 tcatttgtag agtttatgca agaaggttat gaggtaaaag catacagata tatactaaag
    12481 ccaataaata aagaaaaaat ttctaaaagt gtattacctt gtataaatga gatgatgaaa
    12541 aagagaaata attacttaac tataaatgta aaaaattatg tagaccgaat taaaatagat
    12601 tctattactt atatagaaac agatagaccg aatatattga tttatacaca tgatgatatg
    12661 tatattacta aaatgagtat atctaaaata gaaaagatat taaatgaata tggatttttt
    12721 agatgtcata atagctatat agttaattta aaattagtag aatcaatgag tggaagtact
    12781 gttatagtag atggcaaaag tattccaata agtaaatata gagttaaagg cttgaaactt
    12841 gctataacta atatattagg ggatatagta tgctaacaaa tctaagtata ggattttgga
    12901 atattattaa tttagtttca ggtactatag attggattat atttttttta gtaatccact
    12961 tagttggaaa gaggaaaata tctaatataa gatatatatt tacaacaata attatattta
    13021 tatttatggc atacataaat gtagttaaag tgtttccaaa tattaagatt cttctatgta
    13081 tggtaatagg aatatcattt tatataacat catatgaaga taaaatatat aaaagtataa
    13141 tagttagttt attgttttgg ctaggattaa tgattgctga aggttctgca gtaggtctag
    13201 tagtatttat aaataaatta aataatatta acataacaca acatgaaaac ttatttagaa
    13261 tacaagctat tattatgtca aaggtatttt tatttatagg ttttatattg tttaagtatt
    13321 ttaaattatc tttagatttt aaacctaaag atatgatact aataggaata cctatattat
    13381 ctaatatagt tagcttatta cttatatttg aatataattt aagagtcaat acaattacta
    13441 cagaaaactt atttatattt atatttgcaa ttttattgat tttatcatca agtataatac
    13501 ttttaatagt aatagggaaa attgtacaag acgataaatt aaatctagag tatgaactta
    13561 taaatgaaag aataaaaaca aactacagga attatgaaaa tataaacgaa gttcatgata
    13621 gattaaaata tgtttatcat gatttgaaaa accatatgat ttgtataaaa aattatgaca
    13681 ctaaagaaga aattatatca tatatagata acttagagtt tcagataagt gaatttgaac
    13741 attttaaaaa tactggaaat aaaacattag atttaatttt agcagaaaaa ataagtattt
    13801 gtaaaaagta tgatattcaa attgaagata acataaatat atctaaatta aactttattg
    13861 aaaataatga tatatgttca atatttgcaa attcactaga taatgcaatt gaagcatgta
    13921 tggatataaa taatgagcta gaaaaaagaa tagaagttaa agctacatat ataaataaat
    13981 ttgcaataat aaaatttata aatactaagg taaatgatat taaacttatt gataaacgta
    14041 ttcaaactag taaggatgat gataagatac atggaatagg acttgctagt attaaatata
    14101 tagtaaataa atatggtggt gaaacaatag ttaattactc agataatgag tttatattaa
    14161 aaataatgat accaataaaa agttaggaga gtacaaaatg aaaaaaatag cgttaaactt
    14221 acttaaaaac atctcagcat taagttttgg gatagctgta ttatcagcaa acagtgcaag
    14281 ttcttgggta gcacatcagg caaaagagcc acaagcttta caaaaactaa aaaaataaca
    14341 ttaataaaag gacctcttct tattaaagga gaggttttac atgttgagga ggaaatatgt
    14401 ttaaaagatt atcatataaa tttgccaata tattagttaa taatgagatt gtagaaagtg
    14461 aagattttga aatatataga tatggatttg aaactttgat ttatttcata gttaatattt
    14521 cggtagcatt atttatagga attatttttg atagatttat acatacagtt atatttttaa
    14581 gttgttattg cactttaaga cagttcacag gaggttatca tgctaggaat tataaagagt
    14641 gtacattaac atttgcagtt atttatttga taactatttt ttcagctaat aatatagata
    14701 taaataaata taagtattta ttagtattat taatgattat tagtatacta acaatttaca
    14761 agttagctcc tttagaacat agaaacaaac ctcttagtga aagtgagaaa aagcattata
    14821 gaaagactgt gcaaaaaata ctattcgtaa taatatgttt aattatttta tgtaaaatac
    14881 ttaatatttt tcagcaatat gtgatttatg caataatttc tatatattgg attgctatat
    14941 taatatatat tggaatgaag gttaataatg atcagtaaga ttataaaaca ttttctaaat
    15001 taattaattt cactaattaa tattaatttt aaaaaataat gtcaaggtaa atatttgaac
    15061 ttattaaata tagttgaatc tattgtaagt tgtatcaaag gattggctag gaggggcata
    15121 aatatgagta gtattaaggt tatatcagac tttataacca aaataattag aaaaaatatt
    15181 ttatttgcaa taacagttat tattataaat taccatctca tacaacgatg gacacatata
    15241 tccaatagag gaatttcatt taatgattac ttatccttga gtgtatatac ttgtatgaca
    15301 attagtattt taaccatgtt aaatgtaatt ttttatatga gaaaaagtta tgatactgta
    15361 aaaatattat gtagaaataa ttttgattat ttttta
//
LOCUS       DAELSY010000095        14869 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.095, whole genome shotgun sequence.
ACCESSION   DAELSY010000095 DAELSY010000000
VERSION     DAELSY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14869
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.095"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            54..830
                     /locus_tag="KPG09_002566"
     CDS             54..830
                     /locus_tag="KPG09_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0967166.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IVKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            846..1211
                     /locus_tag="KPG09_002567"
     CDS             846..1211
                     /locus_tag="KPG09_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0967167.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(1377..2126)
                     /locus_tag="KPG09_002568"
     CDS             complement(1377..2126)
                     /locus_tag="KPG09_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG0967168.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     GKPFIFVCPTYAWRLPIVVTDFIKETKFLGDNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNS
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(2571..3128)
                     /locus_tag="KPG09_002569"
     CDS             complement(2571..3128)
                     /locus_tag="KPG09_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG0967169.1"
                     /translation="MRTLKYAILGLLNRNPMTGYDIVKEFNFQLAEFWNAKHSQIYPE
                     LKKLVNEKLIVYDIKISGDVLEKKLYTITEKGKDEFLKWLEKDEPMERTPKDIFRLRM
                     YFSNNLDIDTRLILLEHQLLQHQERITFLYSQMERYSEVPPLDSNDFGDYIVLDGAII
                     REQGAIKWLTNCINHHKNLDNTTLK"
     gene            3380..4678
                     /locus_tag="KPG09_002570"
     CDS             3380..4678
                     /locus_tag="KPG09_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HBG0967170.1"
                     /translation="MNLTQKRWIILIASCFINLCIGSIYAWSVFASPMAEHLNSLAGT
                     TFTAASLAIVFTVTNSVGPITMITGGMINDKFGPKKVVFVGGLIFGIGMILAGFSKGL
                     PMLILSYGIITGLGVGMVYGCTISNSIKFFPDKSGLVGGITTAAYGFSSVIMPPVANW
                     IINKYGVTSAFKSIGLVFLIIVCVSSFFIKKCPADFTPEGWVPPEKKGKVRVVINKNW
                     KEMLSTPIFYLMIIILTCGAFYGLMCVSLASSLATNMIGMTSSDSAIVVSILALFNTL
                     GRIVAGYISDKIGRINTLSGALVISIFGLLCLLFSREGDVATFYIGIIIIGICFGSFM
                     GIFPGFTVDKFGPKNNSVNYGIMFIGFAFAGYIGPAVMKNVYSVDGNYQRAFIIAIII
                     ALIGFILTFLYKYINKIQNLKIENLIKGDIKNGKKISKSM"
     gene            4650..6599
                     /locus_tag="KPG09_002571"
     CDS             4650..6599
                     /locus_tag="KPG09_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG0967171.1"
                     /translation="MERKYPNLCKPIKIGNVTFKNRMFSAPMGGTDITADCTIGSKST
                     AFYELRAKGGAGAVTISECMVHPETDGSHAYHLDLKIVDSLASFTYTADAIRRHGAIP
                     SVELSHSGQYSGTYLVDKDKKRGLSQWGVSPSVRPDGLEIKELTEEIIAEIVESYGNV
                     AALAKRAGFEMIMIHGGHGWLINQFLSPYFNKRTDKYGGSLENRVRFAKEVLDSVRKA
                     VGPGFPIEFRMSGSELFEGGYDLDYGTEIAKLLESRVDLLHVSAGTYQRGFAVTHPSM
                     FLEHGCNVYLAEEIKKHVNIPVATIGALNDPEMMEEIIASGKADVIYMARALLADHEL
                     PRKVMSNQDEKIVKCLRCFTCMAERATTSTRRCTVNPLIGRELDGTEVVSVLKPKKVL
                     IAGGGPGGLQAAITAVKRGHKVILCEKTNELGGILKGEQALPFKYKMYELGNTFGKIA
                     KDLGVEVRLNTTVTKEYVENENVDALIIAVGSEPLVPPIEGLDGENVVIVNDYYVEKE
                     KVTDEVIVLGGGLAGCEAAIHLAQEEKTVHLVEMRAELAPDANIRHRPILLEEIERQG
                     IYVYTEHKGLSVTTEGVVCMNKSGNKINIPGTSVICALGQKPRREVVDTLLDSAPYVA
                     QIGDCVRASTITNAVYQGHHAALDI"
     gene            7036..7173
                     /locus_tag="KPG09_002572"
     CDS             7036..7173
                     /locus_tag="KPG09_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0967172.1"
                     /translation="MINENLKSLRKIHQYTQEELAEKLNVSRQSIAKWESGVSQTKGY
                     Y"
     gene            7401..7775
                     /locus_tag="KPG09_002573"
     CDS             7401..7775
                     /locus_tag="KPG09_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBG0967173.1"
                     /translation="MFNLIEMLFPIMFILIFIIIVFTFVKGIATWFKNNNSPRLTVSA
                     RIVAKRQNTTHNNQPNAGDISGAHGFHTISSTSYYVTFQVDSGDRIELSVSGSEYGVL
                     AEGDKGQLTFQGTRYLTFDREN"
     gene            7791..8438
                     /locus_tag="KPG09_002574"
     CDS             7791..8438
                     /locus_tag="KPG09_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967174.1"
                     /translation="MNKFLSQIRRIDNEISITRKIINTIFILCFGIVLGTFAKFLDTT
                     ASNTLPFIFEYLDISNFFGRFAIWLLIALYIAIYSHSSIRASLNVLVFFIGMVSSYYL
                     YSYFVAGFFPKNYAMIWLGFTVISPLLAFICWYAKGKSKISFILSVIIIAILFNFTFI
                     YGWIYFDVYSILEVIVFGCALIALKRNTFRETTYMILSAVVIAVILNMLVPFHFG"
     gene            8818..9897
                     /locus_tag="KPG09_002575"
     CDS             8818..9897
                     /locus_tag="KPG09_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG0967175.1"
                     /translation="MNDMKFIETLKQKRNVCDYSQSRLAQELQINRQNLNEIENGKTK
                     ASKEMKHILLHYLDYCNCTQPFTLTIDYLRVRFPTTDALEIIKNVLAMKSEYFIHEDY
                     GMYAYEEQYIYGDISVNASKDSSMGVLLELRGMGCRDLEYVLQVRGIDWYCFLRCCID
                     YQGVFKRIDLAVNDMGGLLDIEILRERYYANKVWKRSRTHEAVDSGKLSGTHGDTAKT
                     FYIGSKNSSIYFCLYEKEKEQKSKGIKTDIKNRFEIRLKNGKAGQTVEQLVFSRNPEQ
                     TIASFILTQIDFPDYILWDIFLDNVTTSLPFIMTPVAVNMDKIKRWLERQVMPSLLMI
                     KEIEKKTGANYLEEIDRHTRLTEKQ"
     gene            10719..10932
                     /locus_tag="KPG09_002576"
                     /pseudo
     CDS             10719..10932
                     /locus_tag="KPG09_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
     gene            11044..12657
                     /locus_tag="KPG09_002577"
     CDS             11044..12657
                     /locus_tag="KPG09_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG0967176.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFERPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIVEKIFSLAYQGYGSAKITKVLREEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     ATNTANKTPYSYYACSYYGQFGKGTCSMHYIRYDVLYQAVLKRLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASAFKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITNLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEAITNEDNERIQEIEIYYRFIGKVD"
     gene            12686..13009
                     /locus_tag="KPG09_002578"
                     /pseudo
     CDS             12686..13009
                     /locus_tag="KPG09_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
     gene            13041..14228
                     /locus_tag="KPG09_002579"
     CDS             13041..14228
                     /locus_tag="KPG09_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBG0967177.1"
                     /translation="MSKIVFFSILAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIK
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFITHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            14420..14824
                     /locus_tag="KPG09_002580"
     CDS             14420..14824
                     /locus_tag="KPG09_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBG0967178.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYHALYVNKVMLQI
                     YDVENNNKLLRSNEELKSVKYPPIYLKYKPDK"
BASE COUNT         5433 a         1936 c         2752 g         4748 t
ORIGIN      
        1 tgtctacgtg ttataatagt ttaaactaat aaacgaaagg tagatgaatg aatatgaaat
       61 accaacatat aatagaagtt gacaaagaac tttgcattgg atgtgggctt tgtaaaaatg
      121 attgtccagt aaacaatata attatagaaa ataaaaagtc agtaattaaa aaacaagatt
      181 gtctaatgtg tggacattgt gcagcaatat gtcccacaaa agctattacc ttgacaggat
      241 ttgatgaacc tcctatagaa cttacaaata agccaaagct agattcagat gaattactaa
      301 tggcaattaa atctagaaga agtatacgta aatttaaaga caaagaagtt tcttcagaaa
      361 ttgtaaagca aattattgag gcaggtaggt atacccctag tgctaaaaat tcacaagatg
      421 tatcatatat tgttttagac aataaaaagt caatttatga aaacgaagca gtgaaatttt
      481 ttagaaaaat aaaacctatt gcaaatatag ctattaaata ttcaaaagaa gttgagattg
      541 acgataactt tttctttaag catgctccta ttgcaataat gataattaca aaagataaga
      601 ttagtggttc acttgcagca tcaaatatgg cattaatggc agagtcttat ggtctaggtg
      661 ttttatttag tggatatttt tcagatgtag caaataattc gccaaaatta aaaaagcttt
      721 taggtctaaa acgcagtaat catgttctta caacattagt aattggatac ccagatgtta
      781 aatatagacg tactgcacag aaagaagtag caactgttag atacttataa gagagggtgg
      841 attttatgga aaaagaatgt caacaaagaa tgcaagaaat aacatctaaa tttaaagaat
      901 gtcgcaaagc tatctctgct atgggtgatg agactagaca attgatttta attgctctct
      961 tagagagtga ttttaatggt atacgagtgg gagaaatcac aaaaaaaaca catctttctc
     1021 gtcctgctgt atctcatcat cttaagatat taaaagaagc agagattgtt aatttgcgta
     1081 gagaaggaac gaaaaattat tattatcttg attcaaaaaa atcacaatgg aagtcactaa
     1141 cagagctaat aaatcttata tttgttggaa tacaacatat tagtgaaaat gatataagaa
     1201 aaggtgatta aattatactt gatttaatac aaaaagattg atttaggaac taaaaagttc
     1261 actttgagac actatctttt gaactatacc catcttagga gacagttaaa atatgtaaaa
     1321 ctgtataaaa caatgggtat agttcatttt ataatggttt aaatattaag aatatattat
     1381 tctaaatagt atctttctct gttttgagtt ttatttttat actcaatagc ttcagtgggg
     1441 caaccacaaa tacatgccat acaatgagta cagctatttt tccaaactgg ctttttgttt
     1501 tttaaattaa tattatttaa aggacatagc tctacacatt ttccacagcc aatgcattta
     1561 tcagtataat gaaatccttt ggcactaata attgtcttat aaaaaactgt atttattata
     1621 ccacttttaa ttgtacctcc taagccacta ggagtaatag tttcaaattc attttcattg
     1681 cgtatatcag aaataatcct atatatttgt ttatctgctt ctttaatcat ttgttttgat
     1741 gtttctttgt ctgttgtaga aaataaagct atataattct ctggcatttt aatttctgcc
     1801 atacctttta attgccattc cttgtattta catagttttt gaatatagtt tatggatttt
     1861 gctgtatctc ccccacaagt cattacaaag taaactctgt tgtctcctag aaattttgtt
     1921 tcttttataa aatctgtaac cactattggc aatctccatg cataagtagg acatacgaat
     1981 atgaatggtt tacctgctga tattaattca tctgtttttt cctcttttat aagttgattt
     2041 agtgatatca gttcatcatt taattcttgt gcaattttac gagccacata tcggctatta
     2101 ccagtaccgc taaaatataa aatcataata atatctccct aactattttt tattttaact
     2161 cttaattttt tatttattat agtactttat tgaaatattc tattcaaatg tatgtaaaat
     2221 ttagtagtta agaaaattat aacacattag taatttttat attcattggt aaagaatttg
     2281 attaccatta aatttagtaa caaaatatca actggatttt tatataagaa gagatagtaa
     2341 ttttcatgtg aaagattgta aatataaaga tgattttgaa gggcaataag aagataaaat
     2401 tgatagctta ataaaaaata tttaactttt atattaactg ttttaaatat tataattatc
     2461 tatcttgaat aatcaaccat agaagactgt atacaaagac aaagttgtgt acagtctttt
     2521 ttataaattt aatgtctata attaaataca ctattaaaat caataagtta ttatttcaaa
     2581 gtagtattat ccaaattttt atgatggtta atacaatttg ttagccattt tatagcacct
     2641 tgttccctga taatagctcc atcaagaact atataatcac caaaatcatt actatcaaga
     2701 ggaggaactt cactataacg ctccatttga ctgtacaaga acgtaattct ttcttggtgt
     2761 tgaagcaatt gatgctctag taaaataagc ctagtatcta tatctaaatt attagaaaaa
     2821 tacattctaa gacgaaaaat atccttaggg gttctttcca ttggctcatc cttttcaagc
     2881 catttcaaga actcatcttt acctttttct gtgattgtgt ataacttttt ctctaataca
     2941 tcaccactaa ttttaatatc atatacaata agcttttcat ttaccaattt ttttagttca
     3001 ggatatattt gactatgctt tgcattccaa aactcagcta attgaaaatt gaactccttt
     3061 acgatatcat aaccagtcat aggatttcta tttaataatc ctaaaattgc atactttaaa
     3121 gttcgcataa atacacctct ttctttaatt acatacttat tataacacga tatatttagt
     3181 ttgaattaat taatatagct atgttttaat aagaactttt aaattatgag gaacaaaaaa
     3241 taaataaaac tatatataaa aatgaaatta tgtaaatata aacatgtaag tatttacatg
     3301 tttatattta catatagaaa aaattgtgat aatcttaact aagaaattct atttaataaa
     3361 tttatcgagg aggagagtaa tgaatttaac acaaaaaaga tggattatat taattgcaag
     3421 ttgtttcatc aatttatgta ttggttcaat atatgcgtgg agtgtatttg catcacctat
     3481 ggcagaacat ttaaatagtc ttgctggaac aacttttaca gctgctagtt tagcaattgt
     3541 atttacagtt acaaactcag ttggacctat tactatgatt acagggggaa tgataaatga
     3601 taaatttggt cctaaaaaag ttgtatttgt aggaggatta atttttggta taggtatgat
     3661 attggcaggt ttctcaaaag gattgcctat gttgatttta tcatatggga taataacagg
     3721 tcttggggtt ggcatggttt atggatgtac aataagtaat tcaattaaat tttttccaga
     3781 taaaagtgga ttagttggtg gtataacaac agcagcttat gggtttagtt cagtaataat
     3841 gcctccagtt gctaattgga taattaataa atatggagta acttctgcat ttaaatcaat
     3901 aggtttagta tttcttataa ttgtatgtgt ttcttctttc tttatcaaaa aatgtccagc
     3961 agatttcaca ccagaaggat gggtaccacc agagaaaaaa ggaaaagtaa gagttgttat
     4021 taataaaaat tggaaagaaa tgctttcaac acctatattt tatctaatga ttataatatt
     4081 aacttgtgga gctttctatg gattaatgtg tgtgtcatta gcatcttctc tagcaacaaa
     4141 tatgattggg atgacaagtt cagattcagc aatagtggtt tcaattttag ccttgtttaa
     4201 tacattaggc cgtattgttg caggatatat atcagataaa attggtagaa taaatacttt
     4261 atcaggtgca ttagttattt caatttttgg tttattatgt ttgttattct caagagaagg
     4321 agatgtagca acattctata ttggaataat aattataggt atatgttttg gttcatttat
     4381 gggaattttc ccaggattta cagtagataa atttggacct aaaaataata gtgtaaatta
     4441 tggaatcatg tttattggtt ttgcttttgc aggatatatt ggacctgcag tgatgaaaaa
     4501 tgtatattct gtagatggca attatcaaag ggctttcatt atagctataa ttatagcttt
     4561 aataggtttt atattaacat ttttatataa atatataaat aaaatacaaa atttaaagat
     4621 agaaaattta ataaagggag atataaaaaa tggaaagaaa atatccaaat ctatgtaagc
     4681 caattaaaat tggaaatgta acattcaaaa acagaatgtt ttcagcacct atggggggaa
     4741 cagacataac agcagattgt acaattggct caaaatcaac agccttttac gaattaagag
     4801 caaaaggtgg agcaggtgca gttacaatta gtgaatgtat ggttcatcca gaaacagatg
     4861 gttcacatgc atatcatcta gacttaaaaa tagttgattc cttagcaagt tttacttata
     4921 cagcagatgc aattcgccgt catggagcaa taccaagtgt agaactatct cattcaggac
     4981 aatattcagg aacttattta gttgacaaag ataagaagcg aggattatca caatggggag
     5041 taagtcctag tgttcgtcct gatggattag agataaaaga acttacagaa gaaataattg
     5101 ctgaaatagt agagtcatat ggaaatgttg cagcattggc taagagagca gggtttgaga
     5161 tgattatgat tcatggtgga catggctggc taatcaatca attcttatca ccttacttta
     5221 ataaaagaac tgacaaatat ggtggttctt tagaaaacag agttcgtttt gctaaagaag
     5281 ttttagatag tgttcgtaaa gcagtaggtc caggatttcc aatagagttt aggatgagtg
     5341 gttctgaatt atttgaggga gggtatgatt tagattatgg aacagaaatt gctaaattat
     5401 tggaatcaag agtagattta cttcatgtat cagcaggtac ataccaaaga gggtttgcag
     5461 taacacatcc ttctatgttc ttagaacatg gatgtaacgt atatcttgca gaagaaatta
     5521 aaaaacatgt aaatattcca gttgctacta taggagcatt aaatgaccca gaaatgatgg
     5581 aagagataat tgctagtgga aaagcagatg ttatttacat ggctcgtgca ttactagctg
     5641 accacgaatt accaagaaaa gttatgagta atcaagatga aaaaatagtg aaatgcctaa
     5701 gatgcttcac ttgtatggca gaacgtgcta ccacttctac aagacgctgc actgttaatc
     5761 cacttattgg acgtgaatta gatggaactg aagttgtttc agtattaaaa cctaaaaaag
     5821 tattaatagc aggtggtgga ccaggaggat tgcaagcagc cattacagct gtaaagcgtg
     5881 gacataaagt aatactttgt gaaaaaacta atgaattagg tggaatttta aagggagaac
     5941 aagcacttcc atttaagtat aaaatgtatg aattaggaaa tacttttgga aaaatagcaa
     6001 aagatttagg tgttgaagta cgtttaaata caacagttac aaaagaatat gttgaaaatg
     6061 aaaatgtcga tgcattaatt atagcagtag ggtctgaacc tcttgttcca ccaatagagg
     6121 gtcttgatgg agaaaatgta gtaatagtta atgattatta cgtagaaaaa gaaaaagtca
     6181 ctgacgaagt aatagtttta ggtggaggat tagcaggatg tgaggctgca attcacttag
     6241 ctcaagaaga gaagactgtt cacttagtag agatgagagc agaacttgcc ccagatgcaa
     6301 atataaggca tcgtccaata ttattagaag aaatagaaag acaaggtata tatgtatata
     6361 cagaacataa gggtttaagt gtaacaacag aaggtgtagt atgtatgaat aaatctggga
     6421 ataaaataaa tattccagga acctcagtaa tatgtgcact tggacaaaaa ccaagaagag
     6481 aagtggtaga cactttactt gattctgctc catatgttgc tcaaattgga gactgtgttc
     6541 gtgcatctac aataacaaat gcagtatacc aagggcatca tgctgcgcta gatatatagt
     6601 taaaaagacg tagatagtat tgttttatta taaaaaagga gtgtctcaaa atgaactttt
     6661 tagttcatga gacacttttt tacatgaaat taaatatatt tttatcattt caacaatgaa
     6721 aaaagattat ctttattttg agacagcact tttgttatta tgattttaat tcttattgca
     6781 agttttcatt tgattgatga gtaaaacttt ttaagatgta gaagtattta acaatgtaaa
     6841 tgaccctaac ttaaatactt tagcgaaggt tgacagattt gtgtttgctt ttatctatta
     6901 taacttaagg aaacatgtta cctgaacaat aaatttatat aataaaagta tataaatata
     6961 tatatgaatg ttaagattag ttgatagaat ttaaataata aatccaatat tataaacata
     7021 ggaggtatga aaaatatgat taatgagaat ttaaaaagtc taagaaaaat ccaccagtat
     7081 acacaagaag aattagctga aaaacttaat gtttctagac aatcaatagc aaaatgggaa
     7141 agtggagttt cccaaacaaa aggatattac tgaaataacc tgtgtttacc tgcttgtaga
     7201 gaaaagaata ttgctattaa gccagagata ctacataaat gtgttggtat ctctttttcg
     7261 ttaatgcaac acagtaaatt atttcatttt cctaattatt ttagactgat aagtaacttt
     7321 ttaaatgaag aaaagaaatg acaatactta aaggagcatg gtatacttaa aggagaattt
     7381 cattaaggag gaaaaagaga ttgtttaatc taatagaaat gttatttcct ataatgttta
     7441 tactgatttt tataataatt gttttcactt ttgtgaaagg aatagctact tggtttaaaa
     7501 acaataattc tcctcgctta actgtgtcag caagaatagt agcaaaacgt cagaatacca
     7561 ctcataacaa tcaaccaaac gctggtgata tatcaggagc acatggattt catacaattt
     7621 ccagcacctc gtattatgtg acatttcaag tagatagtgg tgatagaata gagctgtctg
     7681 tatctggctc agagtatgga gtgcttgcag agggagataa aggacagctg acattccaag
     7741 gcacaagata tttaacattt gatagggaaa attgaattta gaggtgcatt atgaataagt
     7801 ttttgagtca gataaggcgt atagataatg aaatatctat tactaggaaa attataaata
     7861 caatatttat actgtgtttt ggaatagttt taggaacttt tgcaaaattt ttagatacta
     7921 cagcaagcaa tactttacca tttatatttg aatatttaga tattagtaat ttttttggtc
     7981 gttttgctat atggttattg attgctttgt acattgcaat ttatagtcat tcttccataa
     8041 gagcatcatt aaatgtgtta gtatttttca ttggaatggt ttcaagttat tacctatatt
     8101 cgtattttgt tgctggattt tttccaaaaa attatgcaat gatttggctt ggttttactg
     8161 tcatttctcc attgttagct tttatttgtt ggtatgctaa ggggaaaagc aaaatatctt
     8221 ttatactttc agtaataata attgcaattt tatttaattt tacatttatt tatggttgga
     8281 tttattttga tgtgtattca attttagaag tgatagtctt tggttgtgca ttaatagctt
     8341 taaaacgtaa cacctttagg gagacaacgt atatgatatt gagtgcagtt gttattgctg
     8401 ttatacttaa tatgttagtt ccatttcatt ttggttaggc agattttaag attattatag
     8461 atagaatata aatttcacga accgccgaag cgatccgcca gcggtggcgg tcgtaagaca
     8521 tatcgccacg ccagcttgct ggcgaggtgg cggttcactt tggcggtttg gcgagcctgc
     8581 gagctttcta agcccccgtt atctaacagg gggggactat tacaaaagac aagagcaaaa
     8641 acaaatataa aactgctgat atgcaacgat ttataaggtt gtaagaactt atttcacttt
     8701 acaccctatt ctttctaccc gacaggtcta aaacagcata tcaatagaaa ataaatataa
     8761 gatacaaggt gtatgctcct atgagtatat gccttttttt gattggagga atgaagcatg
     8821 aatgatatga aatttattga aacattaaag caaaagagaa atgtctgtga ttattcgcaa
     8881 tcacgactgg cacaggaact acaaatcaac aggcagaact taaatgaaat tgaaaacgga
     8941 aaaacaaaag ccagtaaaga aatgaaacat atactcttac attatcttga ttattgtaat
     9001 tgtacacagc ctttcacttt aacgattgac tatctgcgtg ttcgttttcc taccacagac
     9061 gcattggaaa ttataaaaaa tgtgctggca atgaaaagcg aatattttat acatgaagat
     9121 tacggaatgt atgcttatga agaacagtat atctatgggg atattagtgt aaatgcttct
     9181 aaagatagtt ctatgggtgt tttattggaa ttaagaggta tggggtgtcg ggacttagaa
     9241 tatgttttac aggtaagagg gatagactgg tattgctttt taagatgttg tatagattat
     9301 caaggtgttt ttaaacgcat agatttagca gtcaatgata tgggcggatt gctggatata
     9361 gaaattctaa gggaacgtta ttatgctaac aaggtatgga aacgttcaag aacccatgaa
     9421 gcagtagaca gcggaaaatt atcgggtaca catggagata ctgcaaaaac tttttatatc
     9481 ggttcaaaga atagttctat ctacttttgc ttgtatgaaa aagaaaagga acaaaaaagc
     9541 aagggtataa aaacagatat taagaaccgc ttcgaaattc gtttaaaaaa tggaaaagca
     9601 ggacaaacgg tagagcaact tgttttttca agaaatcccg aacagaccat agcaagcttt
     9661 attctcacac agatagattt tcccgattat attttatggg atatattttt agataatgta
     9721 acgacctcac ttccgtttat tatgacacct gttgctgtta atatggataa aataaaacgc
     9781 tggctagaaa gacaggtcat gccctcttta ttgatgataa aagagattga aaagaaaaca
     9841 ggagcaaatt atctggaaga aatcgacagg catacaaggc taacagaaaa gcagtaatta
     9901 aagattaaac aaatgacaac agacatagca gatatgattg agaaagatac cgctgttcca
     9961 caagggaatg acggtatttt ttaatttctt caaaaaaact gcaacaaaat gactgcttgt
    10021 gtacagatat ggtgcgagga aaggaaatct aacttttttt gaaaacaggt cattaaatcg
    10081 gtggctgctg tccctatatg atgtgaaaga agaaacgatt tttaatttca acttaacatt
    10141 tgacagtttt cattccctat atggagcaaa gaaaatcttt tattccatag ttggcagtta
    10201 cggtgttgca tgggtagtta gggtgtgaaa agaaaaacag atttttttca tcaccaactt
    10261 agcaaactga tacttgcttt ccctatatgg tgcaggaaaa gagagtttaa ctattttcat
    10321 ttcaacttag caactggacg tttgctgtac agatatgtag tgaaaagaga aaaagttttg
    10381 aatattggtt acgcttcggc tcttttccaa cgcggtatat aggaaagacg attttcttct
    10441 ttcatgttct ttgacaactg aataaagcaa ttcaatacgt ttgacagaca gcgatccatt
    10501 gaagcagata cgccatgacc ttttccctgc aaaagcgagc gaaccctatt tgtgttcctg
    10561 taaaagactt gtcctctttt atcgccatga cctgttctgt ctgataaatg atactcccgt
    10621 acagccacag cttgaacgtt cagagcgtcg cacgcaatgg gtacggctac ataagaacta
    10681 tgcagaggtg gaattcctgt gggctatgac aaatgccgtt gaattgctta gaaaaaagat
    10741 tatggaaagg gggtatgtgt tattgctgat attgtaaaaa caatgcaaga aaagacaaat
    10801 aataagcgta gcattggcaa cagaggaaaa acaaagattg ttgtaaaaga gcatttttcc
    10861 gaaaagggaa agacaatgga ggaacttctg actgatgtta tgctggaaaa agcaaagcag
    10921 acaatcgcat aaaattggtg cagttgtaag acatagattg aatggcaaaa agtacccgtg
    10981 ttatacttta cttgcaagca ggtattgtaa acacgggtta gttataaagg aggataactg
    11041 acaatgaaac aacagattta caatactgca ctttatctta ggttaagccg agatgatgaa
    11101 ttacagggcg aaagttccag cattaccaca caaagaagta tgttgcgtct atatgcaaaa
    11161 gaacatcatt tgaatgtgat tgatgaatat attgatgacg gctggtcggg aacaaatttt
    11221 gaaagaccga gttttcaaag aatgattgag gatatagagg caggaaaaat caactgtgtt
    11281 gtaacgaaag acctttcccg acttggcagg aactatatta tgacaggaca atatacagaa
    11341 ttgtattttc ccagccataa tgtccgttac atagcgattg acgacggtgt agacagcgag
    11401 aaaggcgaaa gtgagattgc accatttaag aacatcatca atgaatgggt ggcaagagat
    11461 acaagccgta aagtgaaatc ggcatttaag acgaaatttg cagagggtgc tcattatggt
    11521 gcttatgctc cattaggata taagaaacac cctgacatca aaggaaaact gctggttgat
    11581 gatgaaacaa aatggattgt tgaaaaaatc ttttccctag cctatcaagg ttatggtagt
    11641 gccaaaatca caaaggtact gcgagaggaa aaagttccga cagcgtcttg gctgaatttt
    11701 acaaggtacg gtacttttgc acatatcttt gagggaaagc ccgaaagcaa acgttatgag
    11761 tggacgattg ctcatgtcaa ggcaatctta aaaagtgagg tttatatagg caacagcgtt
    11821 cacaatatgc agtctacggt ttcattcaag agcaagaaga aagtgcgtaa gcccgaaagt
    11881 gaatggtttc gagtggaaaa tacccatgaa ccgattattg acaaagaagt gttctatcgt
    11941 gtgcaggaac agataaaatc aagacgtaga cagacaaagg aaaaagcaac accgatattt
    12001 gcagggcttg tcaagtgtgc ggattgtggc tggtctatgc ggtttgcaac gaatacggca
    12061 aataaaacgc cttacagtta ttatgcttgc agttactacg ggcagtttgg caaaggtact
    12121 tgttctatgc actatatccg ttatgatgtg ttgtatcaag ccgtattgaa acgattgcag
    12181 tattgggcta aggcagtaca gcaggacgaa gaaaaggtgc tgaacaagat acagaaagca
    12241 ggcaatgcag agcgaatacg ggaaaagaag aaaaaggcaa gtgcctttaa gaaagccgag
    12301 aaccgacaaa atgaaattga ccgtttgttt gcgaaaatgt atgaagatag agcctgtgag
    12361 aagataacag agcgaaattt tgtgatgttg tctagcaagt accaaaaaga acaaatagaa
    12421 ctggaacagc agataaccaa cctaagagaa gaattaagta aaatggaaca ggatatgata
    12481 ggtgctgaaa agtggattga gttaatcaag gagtattccg taccaaagga actgacagca
    12541 ccgttattaa atgcaatgat agaaaaaatc cttattcatg aagcaataac gaatgaggat
    12601 aacgaaagaa tacaggaaat agagatatat taccgattta ttggaaaagt agactgataa
    12661 agagggttca tatctttaac taagggaaac tggagaatct attcctgata taggtagttg
    12721 cattaaactt gcaaaattgt acaaggtcaa actagatgac ttagttaatc attcggaaga
    12781 aaaaacaggt attatagtac ctcctaaagg aaagtttttc tttggagcag tagtagttgg
    12841 tgaacgtggt cagattgtca ttccaaaaga agcaagagaa gtatttaata taaatgcagg
    12901 agataaatta ttggtacttg gagatgagga aagaggatta ggaattgttc atcaacgtga
    12961 tttgataaat tttataggtc atatgggagt agtacgtaaa gaagaatgat tttaaaaaat
    13021 tataaaagga ggtatgaaat gtgagtaaaa tagtattttt tagcattctt gcatatggtc
    13081 atactaatcc tactattgaa gtagttcgtg aattagtgga tagaggaaat gaggtattgt
    13141 attattcttt taatgaattt aaagataaaa ttgaaggtgc tggagcaaaa tttatttgtt
    13201 gtgataaata ccttccagaa ttactccctg gagatgagaa aaaaattggt aaggattttc
    13261 ctggattaat tgagatgata gtagatacta ctatttcttt agatgaaaaa gtttgtaggg
    13321 aacttaaaga ttttaatcca gattgcattg tatcagattc attaagtttt tggggaaagt
    13381 tatttgcaca aaaacttaat attccatatg tatgctctac aacaactttt gcatttaata
    13441 aacatacagc aaagttgatg aaacaaaact ttatagaaat tatacgtatg atttttggtg
    13501 taagacgcat taataaaaaa ataaagcttt tacaaagaaa agggtatgaa gtaaaaaatt
    13561 ttatttctat agtttctaat gataatgata cggataccat tgtatataca tcaaaagaat
    13621 ttcaaccaat ggtagaaacc ttctctagta agtattcatt tgtaggtcca tcagtatcaa
    13681 agttgattat taaacctaaa gagagaaaaa gaaagttaat atatatttcg cttggtacaa
    13741 ttaataataa aaatgttagc ttttataaaa actgtataaa tgcatttaaa gattctgatg
    13801 ttgatgtgat tatgtctgtt ggtagaagta ctgatattaa aagtttagga aatataccaa
    13861 ataattttga ggttaaaaat agtgttgaac agattgctat acttcaaaaa actgatgtgt
    13921 ttattactca ttctgggatg aatagtgtta atgaaagttt atattatgga gtacccatgg
    13981 tattatttcc tcaacattcg gagcagagaa tggtagctaa gagagttgtt catttgggag
    14041 caggaattat gttaaaagag gataagtccg aaagtattaa aaaagcagta tttcaagtta
    14101 taaatgacaa tgaatataaa gaaaatgcaa ctaaactatc aaaaagcttt tataatgcag
    14161 gaggctcaaa aaaagcagca gatgtaatat tacagattat atgtaatagt agacaagttg
    14221 acatataata gatttgttaa aaatatatat acatacatgc aatttaatca aagtactgac
    14281 agtggggtct tttaacctac cagttggggc atatatattg atgctaaata tgagggatgt
    14341 tatattttaa taaaaaagat aacatccttt ttttagtgcg ttatatcaag ttttaaataa
    14401 taaatgaagg aggattaata tgaaaaaata taaagttatt tttatagtat taattatagg
    14461 tattataagt gtaacagggt ttgttgtata taaaaactgc attaaaccat ggaaagatgc
    14521 tgaaacatat attgataagt atatggtaaa acaagatatt tcaaaagata atataaaaag
    14581 tattacaaaa gaaaaagcca agaaggcaag ttacgaagga atactataca aagtgtatta
    14641 caaagatgac cctcgatatg agtatcaata tttttatagt gataattatc atgctctata
    14701 tgtaaataaa gtaatgttac aaatttatga tgtggaaaat aataataaac ttttaagaag
    14761 taatgaggaa ttaaaaagtg taaagtatcc accaatatat cttaaatata agccagataa
    14821 atgatttatg acaaaataga atgattttta ttaaaattta tttaaaaat
//
LOCUS       DAELSY010000096        14836 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.096, whole genome shotgun sequence.
ACCESSION   DAELSY010000096 DAELSY010000000
VERSION     DAELSY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14836
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.096"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            263..3361
                     /locus_tag="KPG09_002581"
     CDS             263..3361
                     /locus_tag="KPG09_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG0967179.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGINDISHVK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKFKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFIVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMEVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFDDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDILTGLYN
                     YESFCEQTSLMLKRYPDKSYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDVHKLIYKIKNSLNRKKFEYMLVPCFGVYKILDNN
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLRHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            3484..4770
                     /locus_tag="KPG09_002582"
     CDS             3484..4770
                     /locus_tag="KPG09_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0967180.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAISENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(5356..6462)
                     /locus_tag="KPG09_002583"
     CDS             complement(5356..6462)
                     /locus_tag="KPG09_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG0967181.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVTTEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DPLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHSMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(6497..8038)
                     /locus_tag="KPG09_002584"
     CDS             complement(6497..8038)
                     /locus_tag="KPG09_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG0967182.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            complement(8400..9146)
                     /locus_tag="KPG09_002585"
     CDS             complement(8400..9146)
                     /locus_tag="KPG09_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0967183.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCNSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDDGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            complement(9358..10668)
                     /locus_tag="KPG09_002586"
     CDS             complement(9358..10668)
                     /locus_tag="KPG09_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0967184.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            complement(10681..11151)
                     /locus_tag="KPG09_002587"
     CDS             complement(10681..11151)
                     /locus_tag="KPG09_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967185.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            complement(11155..12006)
                     /locus_tag="KPG09_002588"
     CDS             complement(11155..12006)
                     /locus_tag="KPG09_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG0967186.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            12931..13842
                     /locus_tag="KPG09_002589"
     CDS             12931..13842
                     /locus_tag="KPG09_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG0967187.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            14017..14514
                     /locus_tag="KPG09_002590"
     CDS             14017..14514
                     /locus_tag="KPG09_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBG0967188.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNAIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVILAGKYAKFVIVGGQKEVGDFWFKFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
BASE COUNT         5661 a         1854 c         2286 g         5035 t
ORIGIN      
        1 attgaggtgg agatttgtca tttatatgag aattaattgt aaacacataa taagatagtt
       61 aattataaat atattaaatt aggtattaca aaactctatt gtacgattga cacaataata
      121 attctattta ctaaaaaata gaactttatt aaggtactat tttaaaacat aaagaagtat
      181 aatatcattg ccaaatatat agcaaaattt taaaagcttt aaaaattcaa tctggaaaat
      241 gaataaaagg agatatatat aaatggatgt aataaattta atagatgagt tggatgaaat
      301 tgtatatgtt tcttctttag atacatatga acttttatat attaatgaaa ttgggaaaaa
      361 ggcaatggga attaatgata tatcccatgt aaaatgttat aaagcattac aggggaaaga
      421 ttctccctgc gaattttgta caaactctat tttaaaagaa gatgaatatt acatatggga
      481 aaatacgaat actcgaacaa atagacattt tatattaaag gacaagttaa ttaaatggga
      541 gggcaaacat gcacgattag aaatagctct tgatattaca gaaaaggaag atataagtaa
      601 gtcaatagca gaaaaattag agataaagga gttattagtt ggatgtatta aacatcttat
      661 aacagaatct aatttttctg atgctgtaaa tcttgtatta accaatattg gtaaatttca
      721 taaagctgat agagcatatg tttttgaagt atcagaagat agaaaaaaag taactaatac
      781 atatgaatgg tgcaatgaag gtgttgatga acaaaaacag gcactaacaa atcttgatat
      841 taatgaatta aagaattggt ttttattatt cgaacaaaag ggttttgtaa taattgatga
      901 tatagaaaat ataaaagaag aacagccaat agaatatgaa attttaaaac ctcaaaaaat
      961 tgaaagttta atgacatctt cactaaaaca agatgataaa gtagtagggt ttattggagt
     1021 tgataatcca cataaggctg ttggagatac ctctcttttg acatccctat cttatttttt
     1081 attaactgag atgaaaaaaa gaaaagtaga aaataaatta aattatatga gctactatga
     1141 cttcttgact ggtgcatata atagaaacaa atatattaag tatatagata attttcaaaa
     1201 aggaaaattt aaatccatag gagttgtatt tgtagatata aatgggttaa aagatattaa
     1261 tgataacttt ggacatgcta atggcgactc tgctattgtg tcagcatgtg taataataaa
     1321 aaaatacttt cattcttata atgtttaccg tattggagga gatgagttta ttgtattatg
     1381 tgaagatata tcaaaacaac tatttgaaga attagtgagt tggttaagtg aagaatttaa
     1441 ggatgagatg atatatagtg tagcagtggg cagtatttgg agtgacgata atattgatat
     1501 acataagcta attaaagttg ctgatgagag aatgtaccaa gataagaaga ggtattataa
     1561 taagaacaaa cttagaaata atgaaatgga agtaattagt gacttagctg attcgacgaa
     1621 taatatttta tttaaacata aagagttagt tgataaggtt tattttgatg atttaatgat
     1681 aaaagggtat tctaaggctt atgaaaaatt agcttataat acatatgata taattttatc
     1741 tatttgcatc gatactaatt taattactcc aatttatcaa tcagagagca atatttttag
     1801 ttttcctata aaggaaaatc ttgaaaaaac tttttcatac ataaaaaaac atttaattca
     1861 tcctgatgat ataatattat ttgatgataa ccaccttcta aaatctcttg atagtcttaa
     1921 taacagcaag caagacgatt tagaactaaa ttttgagtat agacgtttag gaatagataa
     1981 aaagtactat tgggcattaa tttatatgtt aaagattagt agtaaaaata agttaggtga
     2041 gaaacaaaca catattttag ttgctataaa aaatatagat tatcttatgc aactacataa
     2101 aaaccaaaag gatatattaa caggactata caattatgaa agtttttgtg aacaaacaag
     2161 tttaatgctt aaaagatatc ctgacaagag ctatgcaatt ataatgatgg atatagataa
     2221 gttcaaggta attaatgaca tttatggagt tagaggagga gataaggctt taatatatgt
     2281 tgcagaattg attcaaaaaa acattggttc taatggtata tgttgtcgta tgtatgcaga
     2341 tgtattttgt attttttatg agagttactc tgatagagat gttcataaat taatttataa
     2401 gattaaaaat agcttaaata gaaaaaaatt tgaatatatg ttagtaccat gttttggggt
     2461 ttataaaatt ttggataata atattccaat tacaaatgcg tgtgaaatgg caggatatgc
     2521 tcacaaaaaa gtaaagaaac aaagtatcaa taactatata ttttatgatg atactataag
     2581 aaatgatgca cttgaagaaa aacagatgga aagggaaatg gaatacgcac ttgaaaatgg
     2641 gcaattccat gtatatctac aacctaagta taatcttaat gcaaataaaa ttgttggtgc
     2701 ggaagcattg gttagatggg ttcatccaga aaaaggtata atacctccaa ttaaatttat
     2761 ttcactattt gagaagaatg ggtttattat cagattagac atgtatgtgt gggaacaagt
     2821 atgttctttg attagaaaat ggatagattt aggtgaagag ccagttccta tctctgtaaa
     2881 tgtatcaaga ttacatttgc ataatccaca ttttaaagaa attgttttaa gtttaataaa
     2941 taaatatagt ataccaaaat cttttttaga attggagctt acagaaagtt tatttattaa
     3001 aaatataaaa ttatttacta agataatagc cgaccttaga atggatggta ttgttttaaa
     3061 tatggatgac tttggttcag catattcttc attaaatatg ttaaaaaata ttaatattga
     3121 tacattgaag atagattgtg gcttctttaa tgaggaagga atcacagaca gagaaaaaat
     3181 agttttaagg cacactatag ccatggcaaa agatttagat atggatgtag tggcagaagg
     3241 tgtagagaca aaagaacaag ctgaattttt agtgagttta gattgtgtgg taattcaagg
     3301 ttattattat tgtaagccta ttccaagtgc tgattttgag aagttacttt atcaaaaata
     3361 agctaattat cccactctaa atgtaggtag gatggagtag ttcaacataa ttaaatagaa
     3421 gtagaaaata gtgaggatat aatttttaaa aagtataata tttatggatt aggagcgaag
     3481 tttatgatag tgggtattat aggtccttca gactctgggt ttaagataaa ggatgattta
     3541 aaacagatag acagtgattt aaagactaaa atatatgtaa gagaaaaagt agtagataca
     3601 atagaagtta tttctaaatg tgaagatgaa tgtgatgcta ttatatttac aggatgtgca
     3661 gtttatgaat ttataaaatc taagtatgag ataagtaagc cacattcatt tgtaccgaga
     3721 agtgggacga gtataatgaa ggcattttgg gcaataaaaa gtgcaaatat aaaattagat
     3781 aaattcagta tagatgtagt tgatagatat gttgtggaag atgcacttaa agaatttgat
     3841 attaaagcaa cgagtgtatt ttgtaatcca ttttctttgg aagtaggaga gtctgaactt
     3901 gtagaatggc atataaaatt atttgaagaa aataagacgg atataatgtt aactggtttt
     3961 ggtgcagtat ataatgaatt aaaagaaaga ggctatcctg tatttcgttt acaagctact
     4021 atacaattaa tcaaagaaag ttatgataag gtaaagagtg aatatgctct aagcaaggca
     4081 agattttctc aaattgctgt cgaaatattg agtctaatag attataaaga gaagatagat
     4141 aactattatt ctgacatgat aaaaaaatca gatatggata agcttgtagt aaactatgta
     4201 agaagcattc aaggttcctt atttttactt ggaagaaatg attatgttgt atttgttcat
     4261 aaaggggtag ctgataatga gtataattat gataagctat tcaatctaaa gagagagata
     4321 aaagacatgg gcttttctct tagtatagga attggtactg gagtaactgc gtatcaggct
     4381 gaaaataatg cacataaagc attaagacac tccatagatt caaaagaaat tggtatatat
     4441 ttggtagatg aagatgaaaa tataagagga cctttagctt ccgaaaatga attaaattat
     4501 tctttgatgc tatctgatga aaaggtaata gaaatatcta aaaagactgg aatgagttgt
     4561 gagtcagttg caaaaattat tgctataagt gagaatagaa aaagtaagat ttatgattca
     4621 aaagagttag cagagtatct aaatgtaagt gaaagaagtg caagacgtat attaaataaa
     4681 atagtaaatg caggtttagg tcgaatatgt gcaaaagaga ctagtattgg tggaggaaga
     4741 ccaaaaaata ttactgaaat actgttttaa gatattttat aaaactgtat aaatcattat
     4801 attttataaa attctacaac aaattactat ataaagtatt aaagcttatc atgaaaaaaa
     4861 ttagaacaaa ttattatata aaatactaga gcttgttatt aaaaatacta gaataaatta
     4921 ttaatataaa atactagagc ttgttattaa aaatgctaga acaaattatt ataaaaatac
     4981 taaagcttgt tattaaaaat gctagaacaa attattatat aaaatactag agcttgttat
     5041 taaaaatact agaacaaatt attatgtaaa atactaaagc ttgttattaa aaatactaga
     5101 acaaattatt ataaaaatac tagaacttgt tattaaaaat gctagaacaa attattatga
     5161 aaaatattga agcttattat gaaaaatgtt agaacaaatt actatataaa ataccaaagc
     5221 ttattatgaa aaatgttaaa ataaattatt atataagata ctaaaaccta tgtaaaacaa
     5281 ctttaatata taacttataa agttgaagat acataggttt ttatttttat aatttaaaga
     5341 ggtatattat gatatttata atttaatcat atgataaact attttggcag tatcatataa
     5401 ttcgtctata tttacatatt cattaactgt gtgtacatca tacataccta gacttaatat
     5461 agcacaatta tatccaagat tagctaatat atttgcgtca cttccaccac caattaccat
     5521 tggatttgga acaacaccaa cttgtcttat tgcttcttca cttaacttga aaacgtgact
     5581 atctctacta agttcaaaac tagggtaaga catactatgt tcaaaggtat aagtagtatt
     5641 aaatttagat gcagcatcct tacaacattt ttccatatga ttaagctcat actctaaagt
     5701 ttcaggtata tgagacctaa tttcagcagt caatgtaacc ttatcagtta ctatgtttgt
     5761 agctccacca ccttctattt tacctatatt tgaagtagtc aatggatcta ttctgcctat
     5821 gtgcatattg cttattgcat gagaggctac taatatagca tttaaccctt tttcaggttc
     5881 tataccagca tgtgctttct ttccatgaaa agagattttt atagattgtt gagcaggagc
     5941 tttgtatgct atcgaaccaa tagctccacc agaatctaaa atcaccatat ctttacaagg
     6001 caagttgtca ggattaaagt ttttgacacc atgcatacca gcttcctcac agattgtaaa
     6061 acaaagataa atatctctat gtggtatttt ttctgtagta acatgttcta aagcttcaag
     6121 tatagcagct attccagctt tatcatctgc accaagtgta gtagttccat cacttctaac
     6181 tacattacca tctagtattg gttttacacc taaacaaggt tgaacttggt ccatgtgtgc
     6241 acataaacaa agtggtctac ttccttcttc accttttata taggcaatga cattacctgt
     6301 attaccacca tatttttcac ctgcatcatc tataatggca tctatatttc tttcttttaa
     6361 gtagtttacc aaccacttag acatttctaa ttcttgatta gatgggctat caattctaac
     6421 catatccata aaattattta taagtctatt tttatctaac ataaattatt tctcctttta
     6481 gaaagattta aataaattac atgctatatg aaggaagtat tccaggtcct agtggaatat
     6541 taaataccat aaacaatatt aatagtatag tccacactat taagaatgat atagagtatg
     6601 gtatcatatt agcaattaca gtacccatac cagcatcttt atcgtatttt cttgtgaatg
     6661 ctagtataat agggaagtac ggaaataatg ggctaagtgg atttgttata caatcaccta
     6721 tacgataagc catctgagta agtgctggat tgtatccaac catcataaac attggtacaa
     6781 atataggtgc aagaatagcc cactttgcag atgcactacc tataaataaa tttataaaac
     6841 atgataatat aataaatcct atcaacaata aaggtccatt tattcctgct gattttaaaa
     6901 attctccgcc ctttacagct agtatcagac ctaaattact atttgtgaat aattgcaaaa
     6961 attgtgaagc agcaaatgct agtattatat atccacccat atcactcatt gcactagcca
     7021 ttatagagac tacatcctta tccttcttta ttttacctat agtttttccg tatacaacac
     7081 caggtatgaa aaatgctagt gctattatag gtaccatacc tttcataaga ggagcattgt
     7141 ttgatagtaa atctcctgtt tctgggtcag ctaagaatgg ttttttaccg attacactaa
     7201 gagctactat aatagctaca tatattaata tacttatacc agctttttta agccctttct
     7261 tttctacatc actaagactt ccatcaacat ctagttctgc atttccttca tatttaccaa
     7321 atctaggagc tacaattttt tctgttaccc atgttgccaa aagtattaga acaaaagttg
     7381 atgctatcat aaaaaataag ttcattgttg cattggcttg aaatccagaa tcaatcattt
     7441 gagctgctgg aactgtaaaa cttgcagcaa gtatatcatt gacacttatc ataatgtttg
     7501 cggcaaatcc tagacagact gctgcatatc cagaaaataa acctataagt ggatgtctac
     7561 caacaccaag atatacaagt gcagctaatg gtggaaataa tattgtacct gcatctgaag
     7621 ctatattggc aagcatacct atagtcatta caacaagagt tactcttgtt tcaggaatct
     7681 tgcttactga tgctttgatt gttgctgtca taagtcctgt tttgtcacat acaccagcac
     7741 ctatcatagt tactagaact agacccaaag gagcaaaccc ttggaaattg ctaactatgt
     7801 ttcctaaaag aatttgaagc tgttctacag tcaataaatt tactgcagtt atagtttctc
     7861 cagtaccagg atgaactact gatatcccca tagaaccaac tagactagat agtataagta
     7921 aaagtacaca taaccctaaa aatattgtaa ctgggtctgg tagtttatta ccaacagttt
     7981 ctattctgtt taatgtcctg tcaaaaaagt ttttcttctt aactacgtta tcattcataa
     8041 tatcctccct aatgtttaaa aatataatta aaaaataagt attgtggttg atcttacaat
     8101 ataaattctg cttgtcgatt taagacgtgt ccacaatatg tcctaaaaaa agtataaaat
     8161 aaactttttc ctgtgtcaat gcagttttct gaattttctg gttgagtggt gttagttaaa
     8221 tatgctttaa gtagattaat gacgaattat atctaatgta tttaaatagc atatgcactt
     8281 taaagttaat tttaaaacaa aattttatga aaagctagtg gaagattaag ttattaaaat
     8341 aagtagcgta gtaaagtgtt agatttttta attaatcaaa gaaatttact ttgtattttc
     8401 tacaaaaatc tgtgaatatc ttttgaatta tgtagaggat ataaatttat tttatttaca
     8461 tagattagat tttgttctaa tttactgtat tcaaatggtt ttccatgtcc aggataaatc
     8521 attttaggtt ttgtattgat gattgttttc caagattgaa taaattctag tttgctttct
     8581 gcaaatatgg taattttgtg agtacttgga aatccattca ttgcagaatc tccacaaaat
     8641 aaaacaccat catcaattag tagtgaaatt gaatcagcag tatgtcctgg tgtatctata
     8701 atttttccat ttagttcttt ttcaatttct ttacgattct tgtcagacac aagaatacac
     8761 cttttttcaa attcttgttt aagtggagaa aaactatgct caccttttcc aaatagtttc
     8821 ataatttggc aaaagagaaa ggcagtacgg ctgttacaac caccaataaa agagttttga
     8881 cctttatata gaatttctaa agctttatca tgcataacaa tctttgtatt aggagattcg
     8941 tctagaatct gatttagaaa accaacatgg tcatcatgag catgagttag aaatatgtat
     9001 cgaatatctt tgatttttat gttgaattta gaaattcgtt ttttgaaatg tttaaaacca
     9061 ttttcatatc cagtatcaat caagacaaag ccatcttgaa tagggtacaa ccatgtattt
     9121 ataattcgat ttcctgcatt aaacattttt tcaccacctt gaattagaat ataaatttat
     9181 attaattaga aagaatatag catagttcta ctgatatttt aacgtatctc taaaagtttt
     9241 aaggaggata atatatttta gtttaatcta aaagctaaaa gaaaagaagt atactgttac
     9301 aaaaacaaag agtggtataa cttttacagt agataccacc ctattataaa aatagactta
     9361 taaagtttta tcgtgttttt ctaggaatgt tagatattct tctttagtca taactggagt
     9421 aaaggtatct aatatatctt tagcatcctt agcattatca aaaagaaggt ctacaactgt
     9481 aagtgccatt gctttagcag gaattatgta agctaaatct tcatcaacta tttcaaaatc
     9541 tcttgtatga agagctcctt ttacaccacc aatcataggg tgaagagtag gcattatatg
     9601 agacacatct ccaaaatcaa aagagcctgt aaaatctcca ccatcaataa cttttccttt
     9661 tagacctaaa tcttcaagat tgtttttaaa taaattatca agacttttgt atcgtaatat
     9721 tggaagatat ccaggaattt cagttatctc aacatcagct ccaactgcca ttccaccagc
     9781 catcaaagcc ttatttattc tttcattagc atcaatcata ccatttatag ttctagctct
     9841 aacatatgat tccatatgaa catcagcagg aacaacattt actatatctc caccttttgt
     9901 gatgataggg tggaatctaa ctctttcaga ctctttaaaa gtttctctaa gtgcattcac
     9961 gttgtttata gcaagcattg ctgcatttaa agcatttatt ccatcatgtg gagcagaacc
    10021 agcatgagat tctttaccta taaatttaac cttttttcca acaaatccat tactctcagg
    10081 accaactaga gccttatctt ctcctaaatc agaagcatga aacatcatag atatgtctat
    10141 atcatcaaaa taacctcttt ttattaattc ttgttttcca ccaaaataag ttatttttcc
    10201 ttcttttttt aattgctctc tgtattccaa ctcaataaac tcttcagcag gagtagccat
    10261 aaaagatact ttaccatcta aatgttctaa aacgccactt tttactaaac caacagcagc
    10321 acctaacatt ccagctattt gaatattatg tccacaagta tgagaagcac ctatttcatt
    10381 agcatcttta tgttctttac atgatatacc atccaattct ccaagtatag atatatgagg
    10441 acctttcttg ctagaatttg catctgcttt gcatcctgta actgctatat ttttttctac
    10501 agataaattt aattcttgct caaaaaagtt tgaaacagct tctgtagttt taaattcttt
    10561 atatccatat tctggatttt tgtatattcc ttgacctatt tttaatattt tttctctatt
    10621 ttcatctata gtttttatta ctagagattt taattgttct ttattcataa taaattcctc
    10681 ctatatttta ccttgaatac ttaaaactat atgagctata ctcgcacatg caaagaatat
    10741 agttgtaaat acaataagtg aaattataac tattttccat gacatttctt ttaaagcttc
    10801 tattttgttt ccaacagaaa ttcctgcaaa agctagtagt ggagtagtta tagccataaa
    10861 gttaatatta ttagtgttag ttaagaatac ttcagatgtt ggactatatg gcatactaag
    10921 taaaaggccg attatagttg caaatccaaa agctggaaag attgatttag gaaataaatc
    10981 ttttataatc atagctgcca tagcagctaa tattagcatc accataccag gaagagctgt
    11041 aactatagat gtaccaacgc taattctttg tcctataaga attataactc caataacaga
    11101 cataaccaat aattgatatg aacttttctt taaatttaaa ttagcaaaca tttattagca
    11161 ctctccttct gtagatttaa tttctttagt ttttttattt ctcgaaaatt taggttctaa
    11221 aatcttatac ataaaattag taaatggtaa ggatacaaaa actaagaata taattcctgt
    11281 tatactagac atcatattgc ttgttgcagc ataggcaagt attgtatctg ccatttcagg
    11341 tggtaccatg gtagataatg aaccagcagc tgctgtcatc atacttccac taccaacacc
    11401 acaggccatt cccaaagcat atgggtgaag accactataa atagatattg aacctagtac
    11461 actaaagtat atagtaccaa gaactgttcc cattaaatat gtaccaagta caccactacc
    11521 ttctggtgag tttatcccat atttttctga tataactcct aaagaaggtt cacgacttat
    11581 acttgcagta gctccaacag cttctctttt cattccaaac attaatgcaa gaggtaatgc
    11641 aacgattggt gcaagtatat gaccaaattc ttgagcgata aatgctggtc cagcttgaat
    11701 tattttgtca atgtttggtc caactgtagt accatatttt ataccaagca ttactagtgc
    11761 aattccaaca acttcaccag caatatttat ttctttttct ccaaccactt ttttaagtgc
    11821 tttaattttt ttacctaata aatctggtgt tattataagt ccaattataa ctgcataaag
    11881 catagggaac agtactatag aggcttttcc aatagctata gttctttgcc ctataaattc
    11941 acatattact accaagatta agcaagttaa aaatacttga ataaaatgtt ttttattaat
    12001 ttccatgatt tcctccccaa aagaataaat attttactta tctgaaatga gaaaatacgt
    12061 tttcttaagt ataatacgtt ttcctaaata aaatgaaaaa actatttcaa ataagtttca
    12121 gaaatgtcaa taacatgtcc ttaattaagg ataaaacaaa aatttaacat tgtcaacgaa
    12181 aaatatctaa aatattacat attttgaata tattgtaata tagctttatt atgaaaaaat
    12241 tgtttaaata ttgtaggaat aatagctaaa aagctttaaa attagtaata taaaattgaa
    12301 aaataataat atagagttga gaaatagtaa attgaaattt taaaataaat gagtggaagt
    12361 agcttagtaa agtatggaaa tgaatattac aatagaaaat aaataatatg taaagacttt
    12421 aaattaattt taagtattaa gtacaaatac taatttttga tatagaaatt gaataaagta
    12481 aatgataata agcaaatgaa ttaagatgat aataagtgaa ttgatataga aataagtaaa
    12541 ttaagtaaga tgataacaaa tgaaatgata ataagtgaat tgatatagca ataagtaaat
    12601 taagtaagac gataacaaat gaaatgataa taagtgaatt gatatagcaa taagtaaatt
    12661 aagtaagacg ataacaaatg agatgataat aagtgaattg atatagaaat aagtaaatta
    12721 agtaagatga taacaaatga gatgataata agtaaattga tatagtaata aataaattaa
    12781 gatgataata tccaaagtga gaaagataat agaaagtaaa taaaatcatg taagaataat
    12841 ttagataaga ttctaagatt ttatttattt aaaaattagt atgattaatt tagattaaat
    12901 aaatttaata attttagtag gtggtaatga atgaaaataa atagattaac agaaataata
    12961 gtaattctat taaataaaaa attagtaaca gctaaagagt tggcagatag gtttgaagtt
    13021 tctacaagaa ccatatatag agatatagaa actctgtcta tgtcgggagt accagtatat
    13081 atgacgaaag gcaaaggagg agggatatct ttaatagaag aatattctat agacaaggcc
    13141 atactgtcaa aaaaagataa agagagttta atagttgcac tgaaaacatt acaggcgact
    13201 aagtatccag aaataaattc agtagtaaat aaaattggct caatatttgg agagcagaat
    13261 ttttcaaact ggattgaaat agactttact gaatggggaa gtaattttaa tgaagatgat
    13321 aaatttacaa aaataaaaga agctatttta agacgtaata ctataaattt taattatgta
    13381 aatagtttgt caagccagac aaacagaact gtcgaaccac tgaagcttat gtataagagt
    13441 aagacttggt acttgtatgg attttgtaaa ctaaaagatg attttaggat atttagaatt
    13501 agtaggataa ggaatttatc tataaaggat gaagtatttt caagaaagat aatagaagaa
    13561 gtatgtttga atgattctaa agtaattaag gaaaatacta taacgttgaa attgaggttt
    13621 aaagaaaaga tgctttttag agtatttgat gattttaata aggatttaat aactaaaaat
    13681 gaagatgata catatgatgt aattacagaa tttcctattg gtgaatggat ttatggatat
    13741 attttatcgt ttggtgacaa tgttgaagta cttgaaccta aagatgttag ggataatgta
    13801 ataactagac taagggagtt gtctaaaatt tattcattat aatatgatgc tagattaaag
    13861 attttattaa atatataata tcttgcattt agtatataat atctcactga gtttaattat
    13921 attaaaaata tgacataaag gtgtcgtata ttataaatta taattgaatt agttcaaaat
    13981 aaaatatatt aataaaaaca aagtgaggta taaagtatga attatgaaat agtagagata
    14041 aatgagaaaa tagttatagg aatatcgaag gagactacaa ataaagatgg acaagcggta
    14101 aatgctatag gtgaattgtg gaaaaaattt atgggcaaag ggatatataa tgctataaag
    14161 ggtaaaaaga atgacaagac aataggtcta tatacagatt accagggtga ttttacaagt
    14221 ccgtatagct ttgttgcatg ttgtgaagta aatagtaatt ctgataaaga gaagaattta
    14281 gaagaattaa acactaataa tactgttgga gaaagtatta ttagtaaagt tatactagca
    14341 ggtaagtatg caaagtttgt tatagtagga ggtcaaaaag aagtaggaga tttttggttt
    14401 aagttttggc aaatggattt tgataggact tatataagtg attttgaaga gtatcaatgt
    14461 aatacttttg atacaaagaa acaagaaatt catatttaca taggaataaa gtagatttgt
    14521 tattactata ttgaatgaag tattgagtat agtacattta ttatagatag ttttactatt
    14581 tataactata agcagtaaat ctgtaaaaga tttactgctt ttttagtaat taatagtatc
    14641 agtatcactt tgaatcttca gacatattcg tcatttaata ttgtttattg ataaatgata
    14701 cttgtaaata taatgatttg ttatttaatt taaaatatga aaaaaagtat tataaacaca
    14761 ttagaatact tctggattta tgagtataca atttgaaaaa tataaaaaat aatgtataaa
    14821 gctaaatcta atttaa
//
LOCUS       DAELSY010000097        14634 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.097, whole genome shotgun sequence.
ACCESSION   DAELSY010000097 DAELSY010000000
VERSION     DAELSY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14634
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.097"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(315..704)
                     /locus_tag="KPG09_002591"
     CDS             complement(315..704)
                     /locus_tag="KPG09_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0967189.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            896..1342
                     /locus_tag="KPG09_002592"
     CDS             896..1342
                     /locus_tag="KPG09_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967190.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEDIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            1606..2331
                     /locus_tag="KPG09_002593"
     CDS             1606..2331
                     /locus_tag="KPG09_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0967191.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCKYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            2318..3562
                     /locus_tag="KPG09_002594"
     CDS             2318..3562
                     /locus_tag="KPG09_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0967192.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMKNIDYGQKILSKYNYDKSIKINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            3642..4319
                     /locus_tag="KPG09_002595"
     CDS             3642..4319
                     /locus_tag="KPG09_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967193.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            4320..6881
                     /locus_tag="KPG09_002596"
     CDS             4320..6881
                     /locus_tag="KPG09_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0967194.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDQNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIKKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGESYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAIEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGFGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNDFNFYDEISSSESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            7102..8376
                     /locus_tag="KPG09_002597"
     CDS             7102..8376
                     /locus_tag="KPG09_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0967195.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(8537..11578)
                     /locus_tag="KPG09_002598"
     CDS             complement(8537..11578)
                     /locus_tag="KPG09_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBG0967196.1"
                     /translation="MFKQRISKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGNTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            11785..12084
                     /locus_tag="KPG09_002599"
     CDS             11785..12084
                     /locus_tag="KPG09_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBG0967197.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QDLINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(12322..14550)
                     /locus_tag="KPG09_002600"
     CDS             complement(12322..14550)
                     /locus_tag="KPG09_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBG0967198.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
BASE COUNT         5564 a         1771 c         2181 g         5118 t
ORIGIN      
        1 tcttagcttt tttatgtgta tgaatatata gtatactctc atataataaa aaacctataa
       61 taccaataaa atatgaaagt tccaatttat taaatgtaaa aaccagaata aaaactaata
      121 taaagtctat aataaaacct aaagataata gttttttaaa ttctttatct aattttataa
      181 agtaattata atttttatgc tcttggtcta tagaaactcc atagatgtgt tttttaccta
      241 caaaataggg cataaaataa aatgtagcat acatcaaaat tagaactgga aatattgtaa
      301 atagacattc aatcttactc ataatatttc acctcatact tgtcaaataa acttgaactt
      361 agtgacaaaa attcttcttt actaatacct gataaataag attttgccac caataattcc
      421 aagtcaagtt tatttttttg acgagttgta ttatctattt tcattggtag ttgagctaca
      481 atagcacctt ttcttctatc aatatttaag aatccctctt cttttaactc attgtaggat
      541 ttgttcactg tatgtaaatt tacaccaata ttttctgcca tacttcttac agatggaagt
      601 gattcattta ttttcaattc tcctgaagct atagccttag ttatttcttc tttaatttgt
      661 acataaatag atttatcact attaaaatct aaatttaaaa ccataaaaaa cctccattat
      721 gctttataaa tgaaaacgat tgttatatac atagtataac ataaaaaata ataacaaaca
      781 caaaatataa gtttatattt tgtaaaaatt gtaatttaaa aaataacata atttttatta
      841 aaataataca gatactattt atagatagaa ataaaaatta gaaaggagaa aaaatatgtt
      901 aacatattta atgaaaagac aaatgcataa aaaagatgat ggaatgagta aaccaatgat
      961 gattacagga gctgtaatta caggtgtagg agcatatgct gtatacaaaa tggtaaaaag
     1021 ccataaatct aattctcaaa tggtcaatac aaattattat ggaaatagcg attatgatga
     1081 ttataaatat gacgaatttg attatgactg tgattgtaga gacacaaaac aagattatga
     1141 atatgaagaa ttaaaaaaaa aagtaaaaga atttaattca agaagaataa attgtgaaaa
     1201 ttgtccacat gtttctcaag aagaaatgat gcaatatgta aatagtgtta aagatgtaaa
     1261 ggaagatatt gatttacatg aagatgctaa aaaagataat atttacaaag aagatgaata
     1321 caaaaaatat gaagaagaat aagtaatatt ttgtataact gaacaaatag taatgtcaaa
     1381 ttatttaata tctagtttta atatgtaagt tgtaacttaa cttgaaagaa tcagtatcat
     1441 tctttagata gttaagttac aacttacatt ttgtttttat aaattttaat agttctttgt
     1501 gactattttg taattttaga aatattttag tatccagggt aattgtgtat aataaaactt
     1561 gtatagatag atagtatgaa attaagcaga tagtttaaaa ttaaaatgaa aagaaaggaa
     1621 atagttatgt taaagttgtt gatagttgaa gatgatagta ctatatcttt tggaataaag
     1681 tatgctttag aacaagaagg atttagtata gatatatcaa aggatttatc tagtggaaaa
     1741 gaaatgattt cttctaatga atatagcatg atactgttgg atgttacatt gccagatgga
     1801 actggttatg agctttgtaa atatataaga ggattctctc aggttcctat aatattcttg
     1861 actgcttgtg atgaagaagt aaatattgta atgggtctcg atataggtgg agatgattat
     1921 ataacaaaac cttttagaat tagagaatta atatccagaa taaaagctgt tttaagaaga
     1981 aaaggtaata cttcagaaga aaataagaaa atacttaaat ttggagattt aagtatatat
     2041 acattagaag ctagagtata taaaaatgat aaagaaatat ttctgacatc agttgaatat
     2101 aaacttcttt taattcttat acagaataaa aatactgttt taagtagaac tcaaatattg
     2161 gaaaaacttt gggatgtaac ttatgatttt gtaaacgaca atactctaac tgtttatata
     2221 aaaaggctta gagaaaaaat aggtgatgat ttatgtgaac caatctacat agaaactgta
     2281 agggggattg gttataaatg gataggaagt gaaaatagtg tttctatata atccagaagt
     2341 gaagaagttt ttaagtaagt atgttacttt gatgtttatt gttatcataa tatctattgg
     2401 atttagcata atcaacgtgt ctttaactaa agagatggtt gtaagaaata atcaagctat
     2461 aatagggact ttatcgagta agtatccaaa cttagaaagt cagatagtag acataataac
     2521 tcaagggaag tctatgaaga acatagatta tggtcagaaa atattaagta agtataatta
     2581 tgataaatct attaagataa actctgagcc tattatttct aaattagttt tagagactat
     2641 caaaataaat ataatattag tatgtataat atttattttg atatttgtgt tggttgttag
     2701 atattttacg tcaatttaca atgatttatc agatatgact aagtatgttt actatagttc
     2761 agagggtaag ccatttgata tgaaaaacaa gaatcaagaa gggcagatag gtcttctaaa
     2821 aactgaactc ttaaagatga cgacaattct taatgaaaag gttgaacttt taaaaagaga
     2881 aaagatattt ttaaacaata caatttccga tatatcacat caattaaaaa ctcctatgac
     2941 ttctttaata atgttgaatg atttattata taatgatgta ccatacgaag taaaaatcga
     3001 ttttttaaat aagataaaaa atcaattaaa tagaatggat tggttgatta aaagcatgct
     3061 taaattatca aaggtagaag caaaagtgat aaattttaaa aaagataagg taaaatttag
     3121 tgaactcata catagagcaa tgctatctat gaaaatacct atggagatta aaaatcaaaa
     3181 actgactata gagggaaatg ataatgtatc ttatattggt gatattgatt ggtctgtgga
     3241 agcactagtg aatataatca aaaattgtgt agaacatact cctgaatttg gaaatataat
     3301 tataacctat aaagaaaatc cattattttc tgaactaata ataaaagatg agggtgaagg
     3361 tatagataaa aaagatatac cacatgtatt taaacgattc tatagaggaa gaagtagttc
     3421 aaaagaagat agtgtaggaa taggtcttgc aatgtcaaaa tctataatag aaagccaaaa
     3481 tggcgatata tatgtaaaca gcgaaaaagg taagggtaca gaatttcata taatatttca
     3541 taaaatgtat gatagtgact aatctgtaat atttaattca ctttatagta atagccaaaa
     3601 cctaatatat gaagtataga atataaatat tggaggtagt tatggaaatt ttaagagtag
     3661 aaaatctaac taaaagttat ggtaaaaatg aaacaaaagt tgatgctata aaaaatgtaa
     3721 gtttatcagt tgaaaaagga acgtttatag caataacagg tccaagtgga agtggtaaaa
     3781 gtaccttatt acatttatta ggaggagttg acagaccaac tagtggtaag gtttatataa
     3841 atgatgttga tatatataat ttaaaaacta aggatttggc gatatttaga agaagaaata
     3901 taggtcttat atatcaattt tacaacttaa ttccagtctt aaatgtgaag gaaaacatat
     3961 tgcttccagc agagcttgac aatagagata ttgatagaga atattttgat gatttaatga
     4021 aaacattagg tctaattgat agagaaaagc accttccaaa ccaattatca ggaggtcaac
     4081 aacagagaac ctctatagga cgtgcattaa ttaatagacc ttctataata ttagcagatg
     4141 agccaacagg taatttagat agtaaaaatt caaaggaaat attagaattg ttaaaattgt
     4201 cagtaaaaaa atacaatcag acactaataa tgataactca tgacaaaaat atggcacttc
     4261 aagctgatag aattataagc atagaagatg gaatcataaa aagtgatgag gtgatgtaga
     4321 tgaatttata tacatcacta accattagat acttaaaaca aaataaaaga agaaccattg
     4381 taaccataat cggtataata ctttcaacag ctttaatttg tggtatagga aatatatttg
     4441 aaagttttat ggattatcaa atgagagaaa ctataaagaa tgatggaagt ttccatgtta
     4501 cattttatga tgtaaataga aaaaatgtgg aatatgtaac gaaatcagct gaaatagaaa
     4561 aacatgcatt taccaaacaa cttggatatt ctaaattaga aaatagtgaa aatgctatat
     4621 taagtataaa gcaatatgat caaaatgcaa taaacggata taaagtttct ataaaagaag
     4681 gtagatttcc agctaaggaa ggagaaattg ttttaagtga aagtattatt aatcttatag
     4741 atgataagtt aaaaatagga gataagataa ctttaaaagt aggggatatg tttgactcaa
     4801 gcaagaaaaa gattgatagt atgacatttc atgaaggtga ttatatagca aatgaaaaag
     4861 atagaacttt taaagttgtt ggtataatta aaaaacctgg gtttgaacgt tataatggaa
     4921 ttgcaacagc tatagcctac tttaatccta tgaataatta caatgataca attaatgtat
     4981 cagtagcagt aagaaatcct aaagatgtgt ataagataag taataaaata agtaaaaata
     5041 tatattcaaa taaagataaa gaagcagttt ctgatatgat aaaatacaat gaacatttat
     5101 tgaggttaca aggtgctagt aagtattcaa atataaatag ctctataaag tctataatta
     5161 tagcagtaac tattttagta atcatatgta caattgctac agtgtacaat tcctttagta
     5221 tttctataaa tgaacgaaaa aaacagtttg ggatattaaa ttctataggt gctacatcta
     5281 gtcaaattaa aagattagtt tttatagaag gtattataat tagtttaatt ggtataccaa
     5341 taggactaat atctggaaca gtagctatag acctattgtt taaaattata aataagtatt
     5401 ttactgaatc ggttgttact aagatgagtt tgcagatagt gtataatcca ataataatta
     5461 ttgttagtat aataatagtt ttgcttacaa tctttatatc aattctacta cctgcaatat
     5521 cggcttctaa tatttctccc cttaatgtga ttaaaaatac tggagagtat aaagtgggca
     5581 aaatcaaaag ctcaagactt attaaaatga tttttaaaac tgaaggagta cttgcatata
     5641 aaaatattag gagaaataga aaaaaattta taataactct tttttcttta atggtaagta
     5701 taataatatt tatatctttt agtggcttta cattactttt actaagaggt gaagaaatta
     5761 gaaattctca aagaaaatat gatttgtatt taactgcaaa gaacacagct aggtctgtag
     5821 aggatactat aagtgaacta gaagatattg atgggataaa aaactttgaa ttggcaacag
     5881 gacaatatgt gtctataaga gtaagtgaaa ataaaataaa taaatccaag gaagatttaa
     5941 tcaggaaata ttatcaaaaa tataagattg gagaaagtta tgaatatgat ttcagtaata
     6001 atgaattgat ttttccggga gattttgcag taaaaaacaa aataaataac ttagtacagg
     6061 gctcttttaa taaagagaga gcaattgaag aaaatggtgt tatattagtt agaaaaagtg
     6121 caattgaaga aaatgggaag aaaggtgtag tagaacttac caactataaa gtaggagata
     6181 cagtaaattg tgaatatctt gatgaaaatg ccttaagtaa aaaagtaaag gtcaagattc
     6241 ttgctataac tgatgaagaa cgtctaggtt ttggttatca aaatatgggg ttacaattta
     6301 taacttatga tgaagttgct aaaaatttaa atttaaaatt aagtaaaagc cttatattta
     6361 ttgattcagg aggaagcatt aagactaaga aggaaataga agctttggca aataaaaatg
     6421 attttaactt ttatgatgaa atttcaagca gtgaaagtga aaaacaagat ttaaaggtta
     6481 ttaaaatatt tgtatatggt tttatagtag tgatatcttt agtgagtgtt acaaatattc
     6541 taaatacagt aagtacaagt ataaatctaa gaaaaagaga acttgcagta atacaatcta
     6601 taggggttac tcctaaagga tttaggaaaa tgatatattt agaaagtttt atatatggta
     6661 tactgtcatt gttgtttgga ataccaatta gtattggtat aattctaatt atgaataaac
     6721 ttatttcagg tgtaattgaa ttttctccga ttataccgtg gacagccata gttatctgta
     6781 tagtaagtgt ttttataata acttttatag cagggtatat accaatgagt aaattaaata
     6841 aagaaaatat aatagataat ataagaagag aaagtatata gatataaaaa taattcttaa
     6901 ctatataaaa tattattaaa atcatttaag tggtataaaa tattattata tcacttaaat
     6961 gattttttat ttatataaga aatctaataa taaattacta gaggtattta actatttaga
     7021 aagaattggt attatataaa gtaagagaat atataataag aaagaatatt tgattttatt
     7081 tgaaaacaaa agaaaaagaa ggtgattact atttctcata taactgcgaa gaatatgtat
     7141 aaaagtttag aggaaagaat aaacaagttt ccacaaggtg ctccaccatc tgatacatta
     7201 tacaaaatac taaatgtact atatacagaa caagaggcga agttagttgc tcagcttcca
     7261 ataaagcctt tcagagtaaa aacagcagct aaaatttgga gtgtaagtga aagtgaagca
     7321 tatagagtat tggataaact tgctagtaag gctctgatac tagatataga agataataaa
     7381 gggaagaaat atataatgcc tcctccaatg gctggttttt ttgagtttgc catgatgaga
     7441 acgagacacg atatagacca aaaacttttg gcagaacttt actatcaata tatgaatgta
     7501 gaagaagatt ttatcaaaga tttattttat tcaacagaaa cgaagcttgg aagagtatac
     7561 gttcaagagg aagttttaac taatgataat gaggttagta ttttagatta tgaaagggct
     7621 actcatataa ttgatgaatc aactcatata ggtataagta tgtgttactg tagacataga
     7681 atgcaacatg ttggaaaagc ttgtgatgct cccatggata tatgtatgac gtttgacaat
     7741 gtagcaaatt ctttaataaa taataaattt gcaagacgtg tggataaaat agaatgtaaa
     7801 gaactacttc atcaagcata tgaacataac ttagttcaat gtggagaaaa tgttaggaaa
     7861 ggggttacgt ttatatgtaa ttgctgtgga tgttgctgtg aagcaatggt agcagctaaa
     7921 agatttggaa atcttcatcc agtacagaca acaagtttta taccaaatat aaaccatgaa
     7981 aattgtgtaa agtgtggtaa atgtataact gcatgtccaa tagatgcgat aagcaaggtt
     8041 aaggaagatg gtaaagaata tataaaaatt gatgaggata gatgtttagg ttgtggagtt
     8101 tgtgttagaa attgccataa aaatagcatt atgttattaa agagagatga aaaaataata
     8161 actccagcaa attcagtaca tagagcagtt ctcatggcaa ttgaaaaagg gcagttacaa
     8221 aatttaattt ttgataataa tgctctggct agtcatagag ctatgggagc tatactgtca
     8281 gctatattaa agcttgaacc agctaaaaag attttagcca gtaaacaatt aaagtcagta
     8341 tatttagata aattgttaag tatgaatgat aaatagaaac taacttaaaa aaggcaaagt
     8401 tttagactta tctatatctt aataaactta tcatttaata tttaaattta atttttacac
     8461 atataaaact tataacttaa ataaaaaaag gctatattca attaatatta cttgaataca
     8521 gcctttttta gttatgttaa gctatttctt ttatttgctt aaatgagttt tcatttccat
     8581 ttccaccaat ttgtgttaca gatttaaatc tcatagtttt aaatagttct ttttgattat
     8641 aatctagtga gtttcctact agcattacag gagatttagt ttttccagcc aaaactccaa
     8701 cagataatcc gtctattaaa tcatcttgtt tattcattcc atttttagca acatataaat
     8761 tgtctatctt agaatccttg taaaattctt ttattacttt tgcattagtt tcatttctag
     8821 tgttacctga tattctctgt ggattttgaa gtttactttc tatattttta gaaactgtat
     8881 attctccacc gattacataa gatttatcaa tctttttgtt tttaaataag ttatttattt
     8941 cagttatatt actattttca ttagtaagta ttataggcat atcattttga gctgatacag
     9001 ctccaatgct tactgcatcg gctaatcctt tttcgccatt aactactgaa atctttgata
     9061 tattgctaat attgttcaat tctttagcaa tgttcataga agtttgatat ctatcacttc
     9121 ctgaaattct agatacagag atattgtttg attttatagt atctaaagat ttttcattta
     9181 tagaagcctc tccaccaact acatatacat ttttaacttt taatctcttt aattcagcca
     9241 aagttttttc atttatttga gagcttccag tcaataatat aggagcgttt ttcttgtatg
     9301 caaatggtgt tgcggccaaa gcatctgcaa ttgctgaatc gtttattaaa actgcatttt
     9361 cagcattttc ccaacctgct tgacttatct taatagaagt ttcatatcta tcagttcctg
     9421 taagtgcact tgcatttact tcatcttgat atgtaacctc aaatctagca tttggacttc
     9481 ctaagaaaac aattacgcct ccttttggca atctaactct attgttcttt gtaactaaag
     9541 aatagtttac aggaacacct ttatcatcat aaacagcaaa agctgaattt tgaggaagat
     9601 taacttctat ttttttattt gcgatatcgt caccaatctt ataccatttt gcataaccat
     9661 tggattctag ttcacaagta aaagattttt ctgctggcaa attagttata gaatcttcac
     9721 taacatacgt tagtgttcca aaagataaat actctgtacc attttcttta tgaagtttaa
     9781 tgtcggataa atcacgacct attacaccag gtatttctat aaaagcattt gagttattct
     9841 catccattat tttagtgtta actatataac ctggagtttc atcagaaaga ctaaaacttg
     9901 cttttacaga gccaaacata tagctttgag aagtatattt ttcatccact agatagtaac
     9961 ctttgccact tctttttttc cacacttctt tgacactatt tgatatattg ttgtcatcta
    10021 ctttttgagc tacatagtat aaacttgctg tctgacctaa tccagggaca tcatcataag
    10081 agctcatatt taaatatgtg atattatttt tttcttttac aaattttaaa caactgttac
    10141 ccttttcaga tacaaatcta tcttgaccta tgtatacgta tttatcttca ggtccatttt
    10201 ctatataagg agaagatact gttaaagtac cattatcatt aacatctact tttatcatat
    10261 ttgaagaagc ataaagacca ctattttctt ttagagagct aggcatttta acttgttgtg
    10321 gcttactaaa tgttttatca ggttttattt ctttaatttt acctttttct tttaatgcag
    10381 atagtaagat ttcttgtcca attatttcgt taagttgact acttccacca gaagaaagaa
    10441 ctgcaactgc catattttca tctggaagaa ctataagatt actgtggaat aataaagagt
    10501 ctccaccttt tgtaagagct tttaaattat actgattaaa tggataagtg ttgacacaat
    10561 cccaccctaa accatatcca agtatagaat cttctccttc aggccataga ccatttaaat
    10621 attctttgtt ttccatagct tttactgaag caggagaaag aataccattt gaatttttca
    10681 taaatgtttg tgcaaatgta catagatttt ctgcacttga atataaacca cctgcaccta
    10741 tagcattaag gttatcttgt ggaactgcat cttcccaata aggtacatat gcttttgcta
    10801 gttttgaact atcaaaacta ttttctgtag tttttgtatt ttgtaagtta agtggattat
    10861 ttatgtattt atcaagaaaa ttagtaaatg acataccaga tactctttct actaaaattt
    10921 cagctaaagt aaatccatca ttacaataaa cggaaaaagc tcctggttta gcttttaatc
    10981 tttgtttttg taattctttt aagaaattat catgaccata agaatcattg tctgctaata
    11041 aaatagtgtt tttaaaacta cttcccatca accctgatga atgatttaat aacattcttg
    11101 gagtgatttc cttgtatcta tcatcagcca ttttaaattc aggtatatac tttacaacag
    11161 gagtatctaa atttaatttt ccatcatcaa ccaacttcat aacagctgtt gtagtaaaca
    11221 ttttactgat agatgcgata ctatacatat tatccttatt taagtttttg ttatcctgtt
    11281 tgctgtaaac tccaccatta ccagaaatct caattttgcc attatcaatc aaagcatatt
    11341 gagcacttac agcaccatac tttgatagca aagtttggat tttctcttta gcaagagctt
    11401 tagtttttgt gtaagattcc gcgtttttac tataatcatg taattctata tcagaacttt
    11461 gatatttttc ggaattatct tttgtattat cagcaaaagt aatatttgga gcagcagtaa
    11521 ataacatact caatactaat gtactagata ataatttaga gatcctttgc ttaaacatat
    11581 tacattacct ctttcccttt ttaataattt ataattagca taacattctt aattatttta
    11641 atcaacagat tattatcaat aaaaaaagct tttataagta tagcttttta tataacagag
    11701 aaaaatataa aaataatatt tgttgttgta taaagaataa agggtatagg taatatataa
    11761 ataaaatgga ggaattttag acatatgtat aagaatataa ataagaagca acttaaagaa
    11821 atgatgaaaa atgaaaagga tgttttatta ttagatgtaa gaactaaaga tgaatttaag
    11881 gaatataaaa tagaaaatgc tataaatata tctttacaag atttaatcaa taatatagat
    11941 gaaatttacg attataaaga taagaaagta gtagtttatt gcagaagtgg tcatagaagt
    12001 gttactgcat gtaatttgtt agctgaagaa ggttttgagc atttatataa tttaaattca
    12061 ggaataattg attatatgag ttaaataagg taagttatac attaaatact atgtatagct
    12121 ctgtttatta agtaggaata tatctaaata tttataagat aaatttagga aagaacgaat
    12181 tttttcgctt ttttctatga cagtttttgt agtaaatatt agtttttaag ttggaaaaag
    12241 gtagtagctt tttagctact accttttaat atgaacttag aatattatta aaaaaatata
    12301 tttaaagaca gaattaattt tttaatgacc agttaaatta ctatcgccaa atttatcatt
    12361 aaatggtttt ataactctat ttataggtcc ttttagaagt acgtttaaac aaatagacag
    12421 aatcataaat agtaatagaa ctagtatatc tttagttaga tttggagcat atataccacc
    12481 aactgcttcc cttgtagcac ctattgcata ggtaaatggc attatagggt ttattctttg
    12541 gaagaatgta ggtgtaactt gtatagggaa tgtaccacca gaagaaccaa tctgaacgac
    12601 taatagtact attgcaattg cttttcctac atttccaaat acagaaacta aagaatacac
    12661 tataaagtta aataccatac tagtaaacat aagcagaact atcaataaaa ttggatgttt
    12721 tgctgttact cctaacaata atatatctcc agttcctatt ataaaacctt ggacaagagc
    12781 tatacttaaa aaagtaagac ctcttccaaa gtaaatttca taaggtttat agtttccatg
    12841 tacagatgta gacaatagtg ctgaaataag taatatacca acccatgtag aaagaacact
    12901 ataaaatggt gtcattgcag aaccataatt ttcaattgga tataatttct ctgttttcac
    12961 ctcaacaggt tcttttaaga aattagactt tgtcaatata tcactttcaa gcaagctgac
    13021 taattttttc atatcttcac cattactaat tttgcttaaa gtatcaatca aatcatccaa
    13081 cattcctttt gctaaaggaa gtctttctct tatcaatgat atactttcct gtgcatttcc
    13141 tgaaagtttt agagaagtag taagtatttc ttcaaccttt ggaagttttg cttctgcctt
    13201 atctaaaaca gtaattatat catttgctac ttttatactg tttgcaaata tactatttat
    13261 aggttttgat atttttgagt caaaattatt taaaatattt aaattaattc ttccaatacc
    13321 atttgataga cttaaaacat tattcaaagc acttatagat ggttgttgtc cagatataac
    13381 tttgttttta atatcattta atgttgatat agatgaatct attttattgc tagagtcttc
    13441 aagattatct attacatcat ccaatctatt atttgatgta agttggttta atttagttaa
    13501 aaagtccacc aaagtatcgt ttaaagattg caaattagaa agtttttctg ataaattatc
    13561 tatcaactta ggtacatctt cactgccact atttactgca tcaattaaat ttaatgtaag
    13621 agaagaagca gaagatgaaa ggtcaaccat taaatttaag tcagacttaa taagaggaga
    13681 aagttcgtct aaattatcat ttgtatcctc taagaatttt tttaagtcac ttgataatag
    13741 ttttgtatca ttcagagtct ttttaattgt aggtaaatca gatttaatat ctttaactgt
    13801 atcagataat tttgaagtag catcagaagc taaatccaca gtcttattta tatggtcaaa
    13861 tctcttttga aggtcaataa gggaattttc aacttttgtc aattttggaa gactgtgttc
    13921 aattcctgca ccaactccgt ttgatatacc aagaacgatt tcactaactg tttttactat
    13981 agtctgatta acctgtagtt gtattgtaga agcaccttta tctgtaatct ttggtgcaac
    14041 tgcatttatt ttttcattaa cagtatagat aatttcgcct tttttaacat catctgtaat
    14101 taaagatgtc aaatcttttg taaaattttt tggaatctta agtgatgcat aataagtacc
    14161 ttttttaaca ccttcaagag cagttttttc atctacaaac tgccaaccta aactcttgtt
    14221 atctttgagt tgttcaatta attggtctcc tacattaatc ttttgtcctg ctatttcatt
    14281 tcctttatct tcatttgtta ctgctacaag catattagca gtgcttccat aagggtccca
    14341 agatgccttt atattgaacc aagcatacag agatggtaaa aaagcaagac ctaaaactat
    14401 tacaagtacg gcataattgc cacgtatatc ttttaggtca cttttaaaaa tttctaatat
    14461 attttttaaa ccctttgaag gtcttaaatt cttgatatta ttccagttta aaatttcttt
    14521 taaactatta gtttttttca aatgtctcac ctcctaaaat attttgataa agaaaaaagt
    14581 tagatttata atcaatttta aattatatta ctttttacac aaatagtaaa gtta
//
LOCUS       DAELSY010000098        14613 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.098, whole genome shotgun sequence.
ACCESSION   DAELSY010000098 DAELSY010000000
VERSION     DAELSY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14613
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.098"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(46..2712)
                     /locus_tag="KPG09_002601"
     CDS             complement(46..2712)
                     /locus_tag="KPG09_002601"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBG0967199.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKAHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKVKKEKFEEMIKSVEERLTNLESKIK"
     misc_binding    complement(2788..3050)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3351..4178)
                     /locus_tag="KPG09_002602"
     CDS             complement(3351..4178)
                     /locus_tag="KPG09_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0967200.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(4226..5662)
                     /locus_tag="KPG09_002603"
     CDS             complement(4226..5662)
                     /locus_tag="KPG09_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBG0967201.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(5749..6618)
                     /locus_tag="KPG09_002604"
     CDS             complement(5749..6618)
                     /locus_tag="KPG09_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG0967202.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGNYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(6776..7429)
                     /gene="phoU"
                     /locus_tag="KPG09_002605"
     CDS             complement(6776..7429)
                     /gene="phoU"
                     /locus_tag="KPG09_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBG0967203.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(7457..8221)
                     /gene="pstB"
                     /locus_tag="KPG09_002606"
     CDS             complement(7457..8221)
                     /gene="pstB"
                     /locus_tag="KPG09_002606"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBG0967204.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(8240..9064)
                     /gene="pstA"
                     /locus_tag="KPG09_002607"
     CDS             complement(8240..9064)
                     /gene="pstA"
                     /locus_tag="KPG09_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBG0967205.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVIIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(9066..9983)
                     /gene="pstC"
                     /locus_tag="KPG09_002608"
     CDS             complement(9066..9983)
                     /gene="pstC"
                     /locus_tag="KPG09_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBG0967206.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(10365..11600)
                     /locus_tag="KPG09_002609"
     CDS             complement(10365..11600)
                     /locus_tag="KPG09_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0967207.1"
                     /translation="MRIFYLEIKRVLHSRRTLILLLLALVLSALMAFLPITYEGINYL
                     DNNGKVVELSGLDAIKYKKTIYANNSEISSGVVTPKKLKHALNIWKKTEEKYGKVGSE
                     DFPLDIYSKSILPIRPLLNRLPEAFANSETGFATELSEINTNDLDSFYEQCKTHLNDI
                     MKIERQTSSAQMKAQELYRKVDTPFEIYPGYSKDAFDYIIFYIFILLIICVAITAPTF
                     SGEYQTQSDSILRCTKHGHTRLAFTKILALFSIFIVMFVVCISIHLAISDLAFGTECL
                     KTSFQMLFSVISLVNINLLQAQILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAF
                     SICLIPMGIGVSWIGYLLPPGGIGLKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVI
                     EIPIFIFLAIRTYCKHQVS"
     gene            complement(11601..12488)
                     /locus_tag="KPG09_002610"
     CDS             complement(11601..12488)
                     /locus_tag="KPG09_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967208.1"
                     /translation="MELKIEHVTKKFKDKIAVEDVCLTLTPGVWGFLGANGAGKTTLM
                     RIITDIMKPTNGKILYNGKNIYIMGAEYRNLLGFLPQEFGFPQDFSVKDYLEYIAALK
                     DVPPREVKGKINNLLEILTLSDVKNKKIIKLSGGMKRRVGIAQAMLNDPKILIMDEPT
                     AGLDPGERVRFRNFISEFSHNRVVLISTHIVSDIEYISTQNAIMKSGKILDVGTTDKL
                     VKKVEGKVWECLISNRDISKYEMNLRIINQRSEKNNQVSIRYLAEKSQIDNSIAVEPR
                     LEDLYLWLFPEAVYSKEGK"
     gene            complement(12633..13748)
                     /locus_tag="KPG09_002611"
     CDS             complement(12633..13748)
                     /locus_tag="KPG09_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0967209.1"
                     /translation="MKKINNFTYRKLRDKYKFIKREKNTEAYLKYKLYIMLAIYTGIV
                     LVLMFMADYTFSNFSNPLVKWLKLRRDVLFFLVFIIGYITIFNFYWKKPWNYLREIIN
                     ATHTIYQQNDELVELSKPLKEIEQQMNQIKMSVLLSQQAVKEAENKKNNLVMYLAHDI
                     RTPLTSVIGYLSLLKEIPNMDNSQKSKYTEVALDKAQYLEKLINELFEITRYNSNQIK
                     INFEKVDLYYMLLQLKDEFYPLLSEKGNRAILKANESLTIKADSEKLARVFNNILKNA
                     IAYSYPNTEIIISAVHKDSKVDIAFRNYGQTIPEEQLSAIFEKFNRLDEARKSDMGGA
                     GLGLSIAKEIINLHGGDITVQSKDEVTTFTISLPTSI"
     gene            complement(13784..14482)
                     /gene="vanR"
                     /locus_tag="KPG09_002612"
     CDS             complement(13784..14482)
                     /gene="vanR"
                     /locus_tag="KPG09_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBG0967210.1"
                     /translation="MSGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQIRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
BASE COUNT         4574 a         2667 c         1624 g         5748 t
ORIGIN      
        1 agtatagaat atatactttt tgtatgtttt tactaattct tatatttatt ttattttaga
       61 ttctaaattt gttaatcttt cctcaacaga tttaatcatc tcttcaaatt tttctttttt
      121 tactttttct tcttcaatta aactttcagg agcttttgct aagaaacctt gatttgcaag
      181 ctttccatta actctcttta tttcgccttc taactttttc ttttctttag acaatctctc
      241 aatttctttt tcaaaatcaa ctaattcatt aagaggtata aatatttcaa caccatcaat
      301 aactactcca actgcatctt ctggaacatt agatttatct tttgctatct ctacatttga
      361 agcagatgct agagttataa aatagtcttt accaagctca atagcttcta ttttttcttc
      421 acttgggatt attatcaatt tagctttctt agaaggtggt acattcattt ctgctctcac
      481 atttcttata cttcttatac cttccatcaa taagttcatc atatcttctt cttcagccat
      541 gttatcttct tcattatatt ttggccattc agatactatt atacatcctt caacagtagg
      601 aagatgagta tatatttcct ctgttataaa tggcatgtat ggatgtaata attttaatat
      661 tttttcaaga acataagtta atgtatataa tgctgcagat ttagcatttg catcttcccc
      721 atataatcta ggtttaacca tttcaatata ccagtcacag tattctgacc aagtaaaatc
      781 gtatatcttt tgaagtgcta ttcctaaatc aaacttatcc atgttattag ttgcttcttt
      841 tacaatatta tttgctcttg atattatcca tttatcagct agagttaagt cctctttaac
      901 actttctcta gtcatgtttt taattatatc ttcatcaata ttcataaata caaatcttga
      961 tgcattccac aacttatttg caaagtttct agcaaattct actctttcca tatagaatct
     1021 catatcattt cctggggaat ttccagttgt taatgtaaat cttagggcat cagctccata
     1081 ttgctcgatt atctccaatg ggtctatacc atttcctaaa gatttactca tttttctacc
     1141 ttgagaatct cttactaatc catgaaccaa aacatggtca aatggctttt cattcataca
     1201 gaacatacct gcaaatgcca ttctaactac ccagaagaat attatatcgt atcctgtaac
     1261 aagtacactt gttggataat aataatctaa agcttcagtc ttatttggcc atcctagagt
     1321 agagaaaggc cataatgcag aactgaacca agtatctaag gcatcttcat cttgtttaaa
     1381 atgagtgctt ccacatttag gacacttatc tggcattttt ctagcaacta ctatttctcc
     1441 acactcttga caataataag ctggtatttg atgaccccac cataattgtc ttgatataca
     1501 ccaatctctt atattttcta gccattgtaa ataagtttta tcaaatttat ctggaacaaa
     1561 ttctaattct ccttttttaa gaatatctat agctggtttt gcaagttctt ccatcttaac
     1621 aaaccattgc tctgataatc taggctcaac tactgtatgg catctataac aagttcctac
     1681 attatgatta tgcgctttta tcttaattag gtaaccttgt tcttttaagt ctgctacgat
     1741 agcttttcta cactcaaatc tatccatacc ttcatatttt ccacaaactt cattcatagt
     1801 tccatcttca ttcatagtat ttaactgctc aagattatgt cttaatccta cttcaaaatc
     1861 attagggtca tgtgctggag tcatcttaac tgcaccagtt ccaaactcta aatcaacata
     1921 actatctgca actattggaa gttctcttcc tactaatgga agtattgctg tttttcctat
     1981 taaatgttta tatctatcat cttctggatt tactgctata ccagtatctc ctatcattgt
     2041 ctcaggtcta gttgtagcta tttctaaaaa gtcttcacta tcttttagtg ggtactttat
     2101 atgataaaag tttccgtcgt gttcttcatg ttcaacttct gcatctgata aagtagtttt
     2161 gcaatctggg caccagttta ttattctatt tcctctgtat atgtgacctt tttcatatag
     2221 gcttacaaaa aattctataa ctgcttcatt acacccttca tccatagtaa atctttcttt
     2281 atcccaatca caagaatcac ctagttgttt aagctgatta gatatttttc ctccaaattc
     2341 atctttccat tcccaggctc tctttaaaaa ttcttctcta cctatttcat actttgtctt
     2401 gccttcttgt ttttttattc tttcaacaac tttaacttct gttgctatag aagcatggtc
     2461 agttcctgga agccaaaaag cttcatatcc atccatacgt ttccatctga ttaatatatc
     2521 ttgaagtgta tggtcaagag cgtgtcccat atgcaattgc cctgttatat ttggtggtgg
     2581 catcattatt gtaaatggtt ttttatctgg atttggttta gatttaaaat acccttcatc
     2641 catccatttt ttatatagcc tagcctcaaa atcttttggg ttataagttt ttgataaatt
     2701 cgtattttcc attgtcctgc ctcctgtatt ttataaataa attttttaaa tatctttaga
     2761 aataaaaaag agctcttcat cctctttaaa aaggacgaaa agctcgcgtt accaccttaa
     2821 ttccaaagct taaaatacat taacatataa ttaaattatt agttaatgtt attttatcaa
     2881 ctttgactct caaagaattt aacggttcta accgtctaaa cttacttata gttcagttta
     2941 gaaactccaa agctacattc agtaaatctt atacttagaa aatcttacag ccagtgaatt
     3001 ttcctctctt ttagtagtat ttacttactc ctcttcttct cagtctttaa tattacatat
     3061 tattatatac aaatattatt ctctttgtca attattttta ttttattttt tctgaatttt
     3121 cttaattatg tctattctct tttatatagt tgttatattt taaaatatgt ttttacagtt
     3181 attctgtctt ataatgtcaa taattgcaag attattttat tttaatttta atgctttctc
     3241 attaatttta tattatatta tccttacagt atacctctta ttcgtatttt ttatattata
     3301 tgttttattt tataaataat ttactttatc ttttactata tttaaattta ttataagttt
     3361 tcttgccaga aatttttagg tgttatattt tctgtaattg gcaatattgt atatcctctc
     3421 tcttttaaga ttcttataat attatttaaa gcatttaaac tttgctcttt ctcatgaatt
     3481 aatactacta attcatctct ttttcttcca taatataata tattacttac tatctgactt
     3541 gtagaagact tccaatcctg tgtatccaaa ttccagtccc aaattaaata atcatttgcc
     3601 ataagtttat tatatgaacc ttctggcata tatggtttac ttccgtatgg taatcttatt
     3661 aattttgaac tctgaccagt tatttcttgt agagtattat tgcatgtatt aaattcccca
     3721 actgttactt ctgggctttt atataactta tgtatatcat gacttacact atgaaaacct
     3781 attgagtttt cctcttccaa gactctcctt acttcttcct tgtgattttt catattttga
     3841 ttaagcataa aaaatgtcgc tttgacatca ttttctttta aaatatcaag tagtgcattt
     3901 gtatattttg atggaccatc atctattgtt atgtatgcta ctttttcaga agtagcccct
     3961 ggaccttttg tttcttcttg agatgctttg tatatgattt ctcctaaagt attactttga
     4021 gaaagcagtt ccagtgtatt ttcacctttt gaacttatat tttcaactgg aacattatta
     4081 attttttcct ccaataatgt tccagttaaa attaaaacag ctataagaaa tatccttata
     4141 aatacatgaa aaacagcctt attatcttta attttcatcc cccatatctc ccttttataa
     4201 aagcataagt ttaagactta aaaagttatt ttttatattt attctctttc ttattacttt
     4261 ttatatctct actaaatatt tttttaacct tttcttcttc tttttctcct aaagattcta
     4321 ctcttttgat tcccaaagaa gttaaaaatt gtaatacatt taaattatca aatggagttt
     4381 ccatattcgt actttcacta tattcatcaa cattcttagt accttcttcc acacttaaaa
     4441 tacgcttcgg acctcctaca cagcctccta cacaccccat tccttctata aatgtagctt
     4501 ctatttcttt atttagaact ttctttagac cgtccatgca ctcttttgtt ccttcaaatg
     4561 ccttttcttc aaattttata tttttgtcaa ttcttctgac tgataactta actgctttac
     4621 ttacacctcc tgttctagca taaactctac ctgaaaggga cgcctctact ctattactct
     4681 catccattat tgctggattt aactctaatg catcaaatat ttcttgcaac tctttaaatg
     4741 taagcacaaa atcaactgca tcttttatat cttctaacat tgcttctttc ttttttgcca
     4801 tacaaggacc tatgaataca actttagcat ctggatttaa aatttttaca gctctcccac
     4861 aagcaatcat aggagaaacg gatggtgata cattctccaa tatctgagga aaactatttt
     4921 gtatcaaact aacccaaata ggacagcaac aactagttat aaagcatcct tcttcgtcct
     4981 ttttcatatg ctcataataa tcatatgctt cttttgcagt aagcatatct gctgccaaag
     5041 caacttctat catatcttca aatcctatct tttttaatgc agttcttaat ttaccagggc
     5101 ttacatcttt cccaaactgt cctacaaaag ctggtgcaac tattgcatat acagggctct
     5161 ttatatcttt taacaatttt atcattggta taaattctat tttatctgaa attgcaccca
     5221 acttacatct tgatacacaa tcaccacaag cactacatac atcttcctca ttacaattaa
     5281 tatgcttact tcttatctta catgactcat cttccttatt attttcatca caatatttgc
     5341 aagcatctga tattacagat attattggtc ttttgacttc atctaaattt acagcttcat
     5401 gtagtgactc ttttaaattt ttatctgtat cttctgtcac atccaatccc atagttaccc
     5461 taattaaatt taccatagta tctttatcaa attttccact aaaataatta tttaaattat
     5521 cctttaaatc ttcatttaaa attttattag gtaagttttc aatatcctct atatcatcat
     5581 tccacactgc ttttacaagt tcgccaaaaa gcttcatttt atatatttct agtattttag
     5641 aattgtcatt tctagtattc ataaaaaata gtacctccat aaattaaact tatatatgtt
     5701 taatatttac aaagatacta ttttttattt attatttaac tactattatt aatatgattt
     5761 atttagcttt ctcaacaact ctatttcaat cattattttc attagatgat agttgaatac
     5821 ggggcttttt tcttcatcta tattccagtc tagcttatcc acattatatg ttttcttaag
     5881 gtatttatat gtatgctctt tcaaataaag tttattttca agtaactcta tagactgcat
     5941 atgaaaatgt atttctctac atatccttaa ggcattctca attttttcta tattagtttc
     6001 actcaaacta taatccaact catcaataag ttttgaatat atttcttcta atttttctac
     6061 ttcttcttca aatttctcta catttaaatt ttcttttcta agaattttat cttcaataag
     6121 taacattgaa agttcttcta tctttttaaa ttcatctata acactattta gataatttgg
     6181 tctagccaca taataattaa caagtactcc aaacaaaaca cctacagatg tatctaacac
     6241 tctatgtaaa gaataataca cagggtcact atttcctaca cctagatgta tagccagaaa
     6301 ggttacacag gcaactacta ctgactttat tttaaacaga ttgcataaat atattgagca
     6361 cattactcct agaccacata aaaaagggtc tccagcttta atgatggcaa atatgaatcc
     6421 tattatacca cctaaaatag taccttccac tctattgatt cctgtcttta aagaaccttt
     6481 taccgtatct tgaacagaaa ttatacatgc tactagagaa taaaacatat tctctactag
     6541 atagttacca agaatcatac ataaagtgac actaattcct gtttttatag tcctcatacc
     6601 catcttttga agtttcaaag ttccacctcc taaaaagcac cttatataaa atacgcatcc
     6661 tcatatttaa tatgttccta gtatatcaca attttttaaa tttacccatt aatcatattt
     6721 ttttatcaat tctttcttaa tttagacaat aaaaaaactg ctttaaagca gttttttatc
     6781 ctatctcaac cattttaccc tcaattgcaa aaattatctt ctcacataca tttgttatat
     6841 ggtctccaat tctttcaagg tatcttccta taagtagaag ttttactcct tggtctatag
     6901 tatttgaatt ttcatgcatt atttttaaac aatcaatttg tacttgttca tacaatccat
     6961 ctacaatatc atctttagtt ccaacatcat aagctatact tgcatcaaat aagattaaag
     7021 cttctctagt ctgagacatc atagacttac attcttttgc catcttagga atatctatca
     7081 gctcttttat ataagcctct ccacctatct tcaacatttc tctagctata tttgctgcat
     7141 aatctcctat tctttccaac tctatcgata tattacctaa tgaataaata aatcttaagt
     7201 cccttgccat tggttgcttt aatactatta gctctatact cttatctctt atttcttctc
     7261 ttaatttatt tatttcttca tcatcttcta ctacttttct tgcaccctct aaatccttct
     7321 ctatcataca agtcacagac ttttctattg cttcttcaca tttttccatc ataagtactg
     7381 tttgttcttt taaagtatca atacttatat ctaaactatt attgaccatt gtctaactcc
     7441 tttatagtta tatattttaa ccaaatctac ctgttatata gtcttctgtt cttttatcta
     7501 caggagtggt aaacatagtt tttgtatcgc taaattctat aacctctcca tttaagaaaa
     7561 aagctgtttc atctgatata cgagcagctt gttgcatatt atgtgtaact atgactattg
     7621 tgtagtcttt ttttaaatcc tctataagtt cctctacttt taatgttgaa attgggtcta
     7681 aagcagatgt aggttcatcc ataagaataa cttctggtct catagcaatt gctcttgcta
     7741 tacatatacg ctgttgttga ccaccagaaa gtcctagtgc agatgattta agcctgtctt
     7801 taacctcatc ccaaatagct gcacctttta agctttcttc tacaatttta tctaattgct
     7861 tcttatctct tagtccatgt gtacgaggac catatgccac attatcatat atactcatag
     7921 gaaatggatt tggtttttga aataccattc ctactttagt acgaagattt ataacatcat
     7981 ctgaggtata tatgtcctct ccatcaacag aaatgtttcc tttaattgtt acatcttcga
     8041 ttaagtcatt cattctattt aacgttctta taaaagttga ttttccgcat ccagatggac
     8101 ctattaaagc agtaacctta ttctctttta tatccatatt tattttcttt aatgcttgct
     8161 tatcaccata aaataaatcc aaatctttta cactcatttt tatcttatcg attaattcca
     8221 ttattcacac ctcttaaatt taataattag ctttatttaa tttcttagca acaaatcttg
     8281 ctagcatgtt tagtgctaat acaattacta ttaaaacaat tcctatactg gctgctgttg
     8341 ctatatctcc actctcttta gtcagtaagt atgagtgtac agttaatgtt cttgcactat
     8401 caaatattcc acctggcatc ttggctacag tacctgctgt aagtaatatt gcagctgatt
     8461 ctccaattat acgaccaact gataagatta ctccagataa gattcctggt attgcacttg
     8521 gaagtactac cttatataaa gtttgaaatt ttgtagctcc taatgccaat gatgattctc
     8581 tataagcttg aggaactgtt tttaaagctt cttctgttgt acgaattatt actggtaata
     8641 ttattattgc aactgtaagt gaacctgcaa ctatggaata tcccattttt aatgtaacta
     8701 caaagaaaat tcctccaaat aatccatata ctatagatgg tatccctgat aaactttcag
     8761 ttgcaaatct tatagctgta actgcttttc cttttttagc atattcttgt aaatatatag
     8821 cagccaatat tcctatagga gttgctacaa ctatagaaac tactactgta tataatgttg
     8881 taattatcat tggaagtata ccaccatcac ctgatgctga ataattgcta aataagaaat
     8941 taaaattcat gttgccaatt ccctttataa atataaatcc cactataatg actaatgata
     9001 ctattgtaaa tagtgctgat agatatacta atgacttcaa tagattctct ttgaactttc
     9061 tcatcttaat ttaccgcctt atttgaaagt ttatttaaaa ctaagtttaa aattaatata
     9121 aatgtaaaca atactactcc agttgcaaat aacatttctt gatgtgtacc aaatgcgtat
     9181 cccatttcaa gtgctatatt agttgtaagt ggtcttacac tatctgttat tgcagttggc
     9241 ataactggtg agtttcctgc aacaagtatt actgccattg tttcacctaa agctcttcct
     9301 actcctaata ctactgcagc taatactcca gatttcgcag caggtagtac aactttgaat
     9361 attgtttcta tttttgatgc accaagtgct aatgaacctt ctttgtatgc ttttggtact
     9421 gctcttattg ctgtttctga tacagatatt attgttggta acatcattac tgctaatact
     9481 attactattg ctaacaaact ttgacctttt ggaagattaa atatactttg tatagttgga
     9541 actatcactg caagaccaaa tacaccatat aatactgaag gtatacctgc taaaagttca
     9601 actgctggtg acattatttt tgctaatctt ttaggtgcta cttctgctat aaatacagag
     9661 gttaatatac caacaggtac tcctatcaca agtgctccaa gtgttgcaaa tattgatgct
     9721 actatcatag atgatattcc aaatttattg gcagaaggaa cccaatctgc accagttaaa
     9781 aagtctataa atgaatatcc ttcaaatata aatggtctta agcctttata aaatacgaat
     9841 cctattatca aaagtaaact tgcaactgct actaaagcac taagtagaaa aatatttttt
     9901 gctacttttt caattacgta ttttgtctta ttgccattat tgtcattttt ttctaaacct
     9961 actaatctag attttactat cataataccc tcccattaaa atatacttat cgattttcgt
    10021 tctgtattca aaatttaatt ttttataatt cgtcttcatc aaatttcctg tacttttatt
    10081 ctactttttt taagttaaat ctatattagg attaagttaa gcaagtgtaa aattatattt
    10141 acttttttaa cattaatttt acttttaaaa ctatatttga catttacttt acaatatact
    10201 gttgtttctc tatattaaaa tagcagatat agataataat acagtttatg tataatttat
    10261 tacctatatc tgctattaag atttgttaac taaatttaac ttttatacta catatataat
    10321 tatcgtatcc tctagtttat atcactatta tttctatcaa attgttatga tacttgatgt
    10381 ttgcaatagg tacgaatagc caaaaatata aaaataggta tttctatcac tgagaaaaac
    10441 agtataatgt gtggtgtcca aaaactcata cctcctaagt gtaagaaatt aaatgatgtc
    10501 atttgatata ataaactaat cttaagacca attccccctg gtggtaatag gtatccaatc
    10561 caactaacac ctattcccat aggaattaag caaatagaaa aagcaatcag catagaagta
    10621 agagaatcct tacattttgc agataaaaat aatgttaaac taaccattgc aagtattgaa
    10681 attagaccac caatagctat aagaatctgt gcttgtagta aatttatatt tactagacta
    10741 atgacagaaa acaacatttg aaatgaggtt ttcaaacatt cagtaccaaa agctaaatca
    10801 gaaattgcca aatggataga aatacataca acaaacatta ctataaatat agaaaataga
    10861 gctaatattt tagtaaacgc tagacgagta tgtccatgtt tcgtacaacg aaggatgcta
    10921 tctgattgtg tctgatactc accagaaaaa gtaggtgcag taattgcaac acatataatt
    10981 aataatataa aaatgtaaaa tattatataa tcgaaagcat ctttagaata acctgggtat
    11041 atctcaaatg gagtatctac ttttctataa agttcttgag ctttcatttg tgcactggaa
    11101 gtttgtcgtt caatcttcat aatatcattt aaatgagttt tgcattgttc ataaaaagaa
    11161 tctaaatcat ttgtatttat ttctgataat tctgttgcaa atcctgtttc tgaattagca
    11221 aatgcttcag gaagtctatt caacaatggg cgtattggta aaatactttt agaataaata
    11281 tctagtggga aatcttcact acctacttta ccatactttt cttctgtttt tttccatata
    11341 tttaaagcat gctttaattt ttttggtgtt acaactccac tactaatttc actattatta
    11401 gcataaattg tttttttata tttaatagca tctaaaccac tcaattcaac taccttgcca
    11461 ttattatcta aataattgat accctcgtaa gtaattggaa gaaaagccat caatgcagat
    11521 aaaaccaatg ccaacaataa taaaattaat gtacgacgtg agtgcaacac tcgtttaatt
    11581 tccaaataaa aaatacgcat ttatttcccc tccttactat aaactgcttc tggaaataac
    11641 cataaatata aatcttctag tcgtggttca acagcaatag agttatcaat ttgtgacttt
    11701 tctgccaagt aacgaataga tacttgattg tttttttcac tacgctgatt gattatacgt
    11761 aaattcattt catattttga aatatctcta tttgatataa gacattccca tactttgccc
    11821 tctactttct taaccagttt atcggttgtt cccacatcaa gaatttttcc acttttcata
    11881 atagcatttt gagttgatat gtactcaata tcagatacaa tgtgagtaga tattaggaca
    11941 acacgattat gagaaaattc tgaaataaaa ttacgaaatc tcactcgctc accagggtca
    12001 agccctgctg ttggttcatc cattataagg attttagggt catttaacat ggcttgtgca
    12061 attcccactc gtcgtttcat tccaccagat aatttaataa tctttttatt ttttacatca
    12121 gataatgtaa gaatttccaa tagattatta atttttcctt ttacttctct tggtggtaca
    12181 tctttcaatg ctgctatata ttctaaataa tcttttacag aaaaatcctg tgggaaacca
    12241 aattcttgtg gtaaaaatcc cagtaaattt ctgtactcag cacccattat ataaatattt
    12301 tttccattat acagaatttt gccattggta ggtttcataa tgtctgtaat aattctcatc
    12361 aaggtagttt tacctgctcc gtttgctcct agaaatcccc acacacctgg tgttaaggta
    12421 aggcaaacat cttctacagc aattttatcc ttaaattttt ttgtcacatg ttcaattttt
    12481 aattccatat ttgaaactcc tctctttatc tacttacctg caaaaacaaa agtgtcttgc
    12541 ttagtattta ttttactaaa caaagacact cataatttta atattaaata gataatttct
    12601 taagaaaatc ctaatatttg atttatattt tactaaatag atgtaggaag cgatatagta
    12661 aatgttgtga cttcatcttt actttgaaca gtaatatcac caccatgcaa atttataatt
    12721 tccttagcta tagaaagccc taaacctgca ccacccatat cagattttct agcttcatct
    12781 aaacgattga acttttcaaa tatagcagaa agttgttctt ctggaattgt ctgtccataa
    12841 tttctaaaag caatatctac cttgctgtct ttatgaaccg ctgagataat aatttctgta
    12901 ttaggatagc tgtaagcaat agcatttttc aagatattat taaacactct tgcaagtttt
    12961 tcagaatctg cttttattgt taaactttca tttgctttta gaatggctct atttcctttt
    13021 tcagacagca atgggtaaaa ttcatccttt aattgaagta acatataata taggtctact
    13081 ttttcgaaat taattttgat ttgattagaa ttatatcgtg ttatttcaaa aagttcattg
    13141 ataagttttt ctaaatattg tgctttgtca agtgcaactt cagtatactt agatttttga
    13201 gaattatcca tattaggtat ttcttttaat aagctcaaat acccaataac tgaagtaagt
    13261 ggtgtcctta tatcatgtgc tagatacatc acaaggttat tctttttatt ttcagcctct
    13321 ttcactgctt gttgacttaa caaaacagac atcttaattt ggttcatttg ttgctctatt
    13381 tcctttaatg gcttagatag ttcaaccaat tcatcatttt gttgataaat agtatgtgtt
    13441 gcattgatga tttctcttaa ataattccat ggttttttcc aataaaaatt gaaaatagtg
    13501 atatatccaa ttataaaaac caaaaaaaat aatacatctc ttctcaactt aagccattta
    13561 acaagaggat ttgaaaaatt tgaaaacgta taatcagcca taaacattaa aaccaatact
    13621 atacctgtat agatagctaa cattatatat aatttatact ttaaatacgc ctctgtattt
    13681 ttttctcttt ttataaattt atatttatct cttagtttcc tataagtaaa attattaatc
    13741 tttttcataa tactacatcc cttcttgaca agaaaaatag gtgtcaaatc ttgtaaccaa
    13801 caccccaaat tgtttttata tattttgctt catctacagt atcacctaat ttttcacgaa
    13861 gatggcgaat atgaactgtt atagtattgt tacttttatt gtaatattca tctttccaga
    13921 tagcatgaaa aagattttct gaatttacca cagagccttt atttttcaat aatacatgta
    13981 gaatagaaaa ctctgttgga gtaagtgaaa gagctttacc atttaaatgg cattcatatg
    14041 tttgggtatt caattccaga cctaaaaaag aaaaaatatg tgaaagttct tcaccagatt
    14101 gtaaacttcc actgtatttc ttatatcttc taatctgtgc cttcactcta gcaacaagtt
    14161 ccaaaggtcg gaaaggttta gtaacataat catctgcacc taaagttaag cctgtaatct
    14221 tgtctgtttc ttcatcctta gctgttaaca taatgattgg ataagtatat ttttctcgga
    14281 ttttttggca taaagaaaat ccatttactc ctggcaacat aacatctaaa attgcaaggt
    14341 caaattcatt taaattaatg cagtttagtg catcaagacc agaataaaat ttataaacaa
    14401 tatagttttc atttgtaagg tagacttcta ttaaatcagc tatatcacgt tcgtcatcta
    14461 caactaaaat ttttccactc atagacttta aacatccttt ctatatatat ttatatatat
    14521 tatactaaat ttaatttttt tatatgattt tttacattag aatttattaa gaaattctta
    14581 atcttattat gtaactattt tattatttaa cat
//
LOCUS       DAELSY010000099        14611 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.099, whole genome shotgun sequence.
ACCESSION   DAELSY010000099 DAELSY010000000
VERSION     DAELSY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14611
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.099"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            421..1182
                     /locus_tag="KPG09_002613"
     CDS             421..1182
                     /locus_tag="KPG09_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG0967211.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNKKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            1247..2029
                     /locus_tag="KPG09_002614"
                     /pseudo
     CDS             1247..2029
                     /locus_tag="KPG09_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            2296..2715
                     /locus_tag="KPG09_002615"
     CDS             2296..2715
                     /locus_tag="KPG09_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBG0967212.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERIFNLKQEKRKAKHKGH"
     gene            complement(2818..3084)
                     /locus_tag="KPG09_002616"
     CDS             complement(2818..3084)
                     /locus_tag="KPG09_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBG0967213.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSMIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            3311..4012
                     /locus_tag="KPG09_002617"
     CDS             3311..4012
                     /locus_tag="KPG09_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967214.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            4094..4702
                     /locus_tag="KPG09_002618"
     CDS             4094..4702
                     /locus_tag="KPG09_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG0967215.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            5026..5490
                     /locus_tag="KPG09_002619"
     CDS             5026..5490
                     /locus_tag="KPG09_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HBG0967216.1"
                     /translation="MKKLVIILISIISVVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFAISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            6127..7143
                     /gene="gap"
                     /locus_tag="KPG09_002620"
     CDS             6127..7143
                     /gene="gap"
                     /locus_tag="KPG09_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG0967217.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYSDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNQKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            7538..8707
                     /locus_tag="KPG09_002621"
     CDS             7538..8707
                     /locus_tag="KPG09_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967218.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            9088..13341
                     /locus_tag="KPG09_002622"
     CDS             9088..13341
                     /locus_tag="KPG09_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG0967219.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKISYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            13490..13984
                     /locus_tag="KPG09_002623"
     CDS             13490..13984
                     /locus_tag="KPG09_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG0967220.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSVYIGDEKFNNLKNECIKK
                     GIDIKISKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKNIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            14100..14477
                     /locus_tag="KPG09_002624"
     CDS             14100..14477
                     /locus_tag="KPG09_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBG0967221.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
BASE COUNT         5934 a         1450 c         2602 g         4625 t
ORIGIN      
        1 tattaggcaa taggaaatct tttcatagtt aaattttcaa atatttttta gggaacttta
       61 taattgagaa ttaagggtat agtatgtgat gaacttacta aatttataat taggttatat
      121 aatggaataa tcatcaatag ttttacttta gggaaaatat atgcttaagg aagtatagct
      181 tactaatatt attaaaatgt atatttaaaa tgataaaaaa agaattagac aagtatttag
      241 taaattgatt atgtaaaatt ctatcaagca ggaaccctat gactttagtc atgggaggtt
      301 cagtaggtaa cttgttgaat taagtttata ttatttaaat aaactactta atttagataa
      361 tataaaaagt cagaatttta agaatagagc tatataatct tagctattag gagaagtact
      421 atgataggaa tatattttag tggaacagga aattcaagat actgtgtaga aaaattttta
      481 caagaatatg atataacagc aaattcttat tctattgaag ataatgaact tttacagcac
      541 atcaataatc atgagaatat tatttttagt tatcctgtgc aatatagtaa tgtgccaaaa
      601 atattgaaag attttataat aaatcattca actttgtgga atggtaaacg tgtttttatt
      661 attgcaacta tggctctatt tagtggagat ggctctggtg tgttagctag aatattaaaa
      721 aaatacggtg ctataactgt tggtggatta catgtgaaga tgccagatag tataggagat
      781 gaaaaggtat taaaacattc tttagagtat aataaaaagc ttgtaataaa agcagaaaag
      841 aaagtaacta aagctgctaa aaaactgaaa catgggtcac caccaaaaga agggttaggt
      901 tttttgagtc atattgtagg gttatttgga caacgattgt attttatcaa taaaacaaat
      961 atgtatacag ataaactaaa aattaatact gaggaatgta ttgtatgtgg taaatgtgtt
     1021 aatttatgtc caatgaagaa tctagtaatt aaaaattgta tgattgttgc aaatgggcaa
     1081 tgtacaatgt gttatcgttg tgtaaataat tgtgcaaaaa aagcaattac attgttaggt
     1141 aaaaaagttg ttgagcagag caatattaaa aaatatatgt aagaaattta aagttttatt
     1201 gaatgtaaat tgatgtatat tttatgttgg aaagagaggt caaataatgg aatactacat
     1261 agttgattca tttgcaacta aactatttaa aggaaatcct gctggagttt gtgttcttga
     1321 cagaataata cctttagagt taatgcagaa aatagcagaa gaaaacaatt taccagaaac
     1381 agcatttgta gttaaaggta aaggaaatta tgaattacga tggtttactc ctaaagcaga
     1441 aattgattta tgtggtcatg caactttagc agcagcttat gtaatatcaa attttattga
     1501 tgttaatgta aaaaggatag atttctttac tcaaagtgga aagcttgaag ttacacgaaa
     1561 tggaaatcta tatgaaatga tatttccaga aataatgcct attgaaatag agttatcacc
     1621 ataacaagct aatttgatag gctgtgtacc aagtgctgtc tattcttcaa gagatttaat
     1681 cttattgcta aacagtgaac aagaagttat aaattataaa cctaattacg cacaattgcg
     1741 taaattgaca gactggttag gtataataat cacaccacaa ggaagtaata cagattttgt
     1801 atcaagatat ttttgtccag aattagattc agaagaccct gttactggtt catcacattg
     1861 taattttatt ccatactggt ctgaaaagtt gggaaaacac aaaatggtag ctgctcaact
     1921 ttctaatcgt ggtggtataa ttcagtgtga agtgttgaag gataatactg taaaaatttc
     1981 tggtgaagca gtcttgttta tgcagggaac aataaagatt gatatttaaa taagtataat
     2041 aactaattta ttatgaaaaa taattaattt tcttagtgtt aacaataata aagtagagtt
     2101 aaaaagcttg atttcactta aatcaatatg atagatgttg atactaagat tgacaagcat
     2161 gattaaaaaa gataatattt aatctataaa gataaaaaca cagtctggtt ggtagtccag
     2221 acgcagcaat agctgtcagt aacctgcctc cttggttgtc cgttctttag aaagtaattt
     2281 aaggaggaac tagcgatgga caaaattagt ggaaaattaa cagtcttatt tgaagaacca
     2341 ttttgggtag gtatatttga aagacaagat ggtaaaaaat atgaagcatg tagggtagtt
     2401 tttggagcag aacctaaaga agtagaagtg tatgaattta tacttgaaag atttttctca
     2461 ttagattttg gtagcattaa attagaaaaa aatgtgacca aagacaacat tggatacaaa
     2521 agaatgcaga gaaaagtaaa aaaggaacaa gaaaaagaaa ctatagggac taaggctcaa
     2581 aatgctctta agcttcaata tgaagaaaga aaacaggata gaaaaaacct agctaaaaat
     2641 agaaaagaag aagaaaaaga aagaatattt aatttgaaac aagaaaagag aaaagctaaa
     2701 cataagggtc attaatttta attaaatatc tttttaataa ttgctagaaa aaggtgtata
     2761 aagtttttaa ttttatacac cttaatttat tgaaaagaat aaggttttat atttcaatta
     2821 ctttaaaata taaaaatata ctaataaaaa ttgtaaaaat agtataaatg gtataccaaa
     2881 tgtaaattta atgtgtttag ttttatgtct aaaagaatac attccaatca tagaacctat
     2941 actacctcca attactgcta tagacataag agtagcttct tttattctcc attcgtttct
     3001 aattgctctt ttcttatcta tgtacattga aaagaaagca atagaattaa tcactataaa
     3061 atatattaga ataaaatttt tcacagtctt gcctccataa aaatatatta tacattataa
     3121 aacaagatat ttgttttgga aagttaaatt tgtaattagt acatataaaa tatctgataa
     3181 gaataaatat aatattaatt ggaaaattta aaaaaattaa acaattagca aatagggggt
     3241 atatttataa gacacaaagt gataaaatct atttagaaaa taatgtaaaa taacaggagg
     3301 ataaagcaaa atgaaattta tagtagaaaa agaagttttt gataaattag aaaatgtatg
     3361 ctttggtgta gtagttgcaa aaggaataga taatactaaa gaaatagaaa gaataagcaa
     3421 ccttttagat ataagtatag atagagtaga agattacttc aaagataaaa aagtaaagga
     3481 atcagaagaa attataccat atagagaggc atttagaagt ctaggcatga acccaaataa
     3541 atttatgagt tcgatagaag caatgacaac aagggttgct aagaataaga aacttcctca
     3601 tataaatcct attgtagatt taggaaactc tatttcttta aaatatttac ttccaatggg
     3661 tgctcatgat atggatttta gaaatgatga tgtatatgtt agattttcta aaaaagggga
     3721 taaatttgta ccatttggag aaactgatgt tgaattaatg gaagaaggag aattaattta
     3781 ttcagttgga gacatggtaa aaactagaag atggatatgg agacaaggag aggaaggaaa
     3841 aattactaat agctcaaaaa atatattttt tcctatagat ggttttactg atgctaattt
     3901 agataaggta atgagtgcaa gagaagaatt agcaaaattg ttaaaggaaa tatttaattg
     3961 tgaaatcaaa gtaggttttg tagataaaga taatcctgaa atggaaatat aataagttgt
     4021 tgaatacttg ttaagatatt atatttactt aatgatgtta aaaaggtatt aaaaaagaaa
     4081 ggtcgtagta aatttgaaaa aaactataat aaagaatgta aatccaatga ttgaaaataa
     4141 gtttatagga ctttttgaaa tagagtataa aaacaaatta gatgaagata aggtttggat
     4201 ggtagcatca agaaaaagta gtgagcaatt aaaaaatatt tatttgaaga atgaagaaga
     4261 tagtgtagat gcagttgtaa tagttgggct tcataaaagt agtaaaaaat taattttaat
     4321 aagacaattt agagtaccta ttaatggata tatatacgag ttaccagcag gtcttgttga
     4381 tgaaggagag tctatagata tttctgttga aagagaactt agagaagaaa caggacttac
     4441 tcttttagaa attaaaaaaa ataaaagtat tgataaagtt tatttatctc ctggaatgag
     4501 tgatgaatct gttgcctttg tttattgtat atgtgatggt gatataacag atgaattttt
     4561 agaaccagat gaagagattg aagcgctttt aatttcgcaa gaagatgcaa aagagatttt
     4621 acaaagtaat cataagatag acacaaaagc atttttgatt ttacaaatgt ttgtgagttt
     4681 aggagctaaa ttatttgaat agatatatag aaaagaggtt atatagaaga gtaaaataat
     4741 aaagtttatt ttacttgtat aaattataat ctcttttttt atacaataaa gtataatttt
     4801 gtgtattttt aaataagtta cttgactctt aaattttcat aatatgttat aaaccaattt
     4861 aagatactac aataattaaa attccaaaat tatttagcat atttgcaatt aaaatgagaa
     4921 aaggtaaagg ctgaatgatt gaaaaatttg atgtaaaata gtagattagt ttaatattat
     4981 agtttgtagg gttaaaaatt agaaattgta aaggagataa attctatgaa aaaattagtt
     5041 attattttaa ttagtatcat tagtgtggtt agcttgacag cttgtaaaaa aagcaatgaa
     5101 agtaaatata ataatttaaa caacaaagaa tcaggacaaa caaccattga aatggactta
     5161 gataaaaatt atgatacttc agacccattt gttaatgcac gtttgttttg tgtgtctaat
     5221 gatatagatg ttttagacac agagatttct tttaaaatgg atggagatag tggtattgtg
     5281 gaaataaagg acaataaaac agatgaaact ttatggagta atacatggca tggaagcgtt
     5341 gataatgata cattcgcaat ttctctttct aatatacaaa aagagaaaga gtatgctata
     5401 tgctttaccg gaattaaaat aaatcatgca gttgtaaagg ttacttttga aagcaattta
     5461 gttaaggaga aagaaaaacc ctcaaaataa attccagctt gtctataact tgatattcag
     5521 tacataaaag cagttgatat ttatgattga ctgctttttc ctatcaaaaa ggataggttg
     5581 tgcattttta cttgaaatag tttatgcaca tcaaaagttg gtgagcatga tattggaaat
     5641 ggtaagcatg aaatcttgat aacacaagga aataaattta agttttcaat taagtatatt
     5701 tccataattt agttaacaat aagctaatta ttatttatat tttgatatat aaataataat
     5761 tgtggcaaat tttaaaaaat catatatttt ttgaactaaa taaaaatttg tttttattat
     5821 tttaaattag aggagattct tgaagatata ttatttttaa aaataagtat agtttaaaaa
     5881 ttcaaaaaaa agatggcaaa atatgtccca agggacattg agtaaaataa atgatttcag
     5941 cttatttaca ctaaaaagta catactaaat gaggggtgaa ataaagaaaa atgatatact
     6001 aattgtcttt tgataataaa aaaacatgga atttgattat ttaatcaaga aaaagtattg
     6061 aacaaatgat ttatttagtg tacaataatc atgtaaaaac atagtaatgg agggagacat
     6121 ttcataatga aaattaaggt aggaatgaac ggatttggaa gaataggaag agctgtatta
     6181 agaatagctc aagaagaatt aggagataat atagaaattg tagccataaa tgcaagagca
     6241 acaacagaat ctttggcaca tttatttaca tatgattcat gctatggaac ttttagaggt
     6301 gaagtagagg caaaggatga agacactcta gtagtaaact ctaaagagat aaaaatatta
     6361 agatatagtg acccagaaga attaccatgg aaagaactgg gggttgatat agtaatagaa
     6421 tcaacaggat tatttacaca aagagaaaag gcagaaaaac atattaaagc aggagcaaaa
     6481 aaagtaatta taacagctcc aggaaaaaat gaagatataa ctatagttat cggagttaat
     6541 gagaaagaat atgataatca aaagcacaat ataatttcaa atgcatcttg tactacaaat
     6601 tgtctagctc cttttgctaa agtattggac gaaaaatttg gtatagtaaa agggttaatg
     6661 actacagtac attcatatac taacgatcaa agaatattag ataaaagcca taaagatttg
     6721 agaagagcaa gagcagcggc tgaatctata atacctacca ctacaggagc agcaaaagct
     6781 gtgtcaagag tactaccaca attagaagga aaattaaatg gtttttcact tagagtacca
     6841 acaccaacag tttcacttgt tgatttagtg tgcgaactta aagaaaacgt tacatctgag
     6901 caagttaact ctgtgttaaa agatgctgct gaaggagaat taaaaggtgt tttaggatat
     6961 tgtgacaagc cacttgtttc tatagattat aaaggagatt caagatcatc tattatagac
     7021 gctctatcca caatggttat agaagacaat atggttaagg tagtttcttg gtatgataat
     7081 gaatggggat attcttcaag aactgttgac ttagtaaaat atgtagctga aaaattaaaa
     7141 taatatatat ttcttaaagc aaattttaaa aaaatttttt gaaaaaatta tataaaacta
     7201 aaaaaaaaga ttatgaaaaa tattgaagga aaaatttttt cataatcttt tttctttcat
     7261 gcaaaaagat acaagttaca acttatattt gcatattagg tcgttagaga agtatatata
     7321 tatttagtaa ggaatcatac tttttataaa atgaaagata agatgtcgaa aacttgcata
     7381 aatctgaaaa atttttatta ttaaaatact gattttcatt ttaaaaatga taaaaaaatg
     7441 acaaataaag ttgcaaatag taatacttat aagtataatt aaaaaagaga aaaggttttg
     7501 ttaaacttta taacaaaaaa agaagggatg attcaaaatg gagaagttaa cagacaggag
     7561 acaggttaag gcagtaagca tggaaacctt cattttccta atattactag tagttggttt
     7621 tgggtatgtt ggtagtatta tgggagcagg aatgatgttc aaagtaataa tgagtacagc
     7681 acatgcttta ctattggaga cagttttctt aattatggct atggcagttc ttgcaggcgc
     7741 attaagcgca ctactttcag agtttggagt aatagcgtta attaataaaa ttttcgctgt
     7801 atttatgaag cctttatatg gactacctgg tgctagtata gcaggagcta taacaactta
     7861 tttatcagat aatccagcta ttataccttt tgctaaagat aaaacattca cacagtattt
     7921 taaacaatac caagttccag cattatgtaa cttaggaaca gcctttggta tgggacttat
     7981 cttaacaaca tttatgattt ctcaaggtac agaatatgta cttccagcat taattggtaa
     8041 tttaggtgca attataggta gcgtcataag tgttagaata atgttgacat ttactaagaa
     8101 gttttataaa tacaatccgg aagaagataa agcaacagga acattagaga aaaaggatga
     8161 atttagagaa atcagagaag gaaatgtatt tcaaagagct ttagatgcaa ttcttgaagg
     8221 tggtaaaatg ggggtagata tgggaatggc tataattcct ggagtcctag tagtatgtac
     8281 attggtgatg ttattaactt ttggaccatc tacagaccct gttacaggtc aagaagttta
     8341 tacaggagct gcatatgaag gaataaaatt acttccagtt attggagata aacttggatt
     8401 tatattagaa ccattatttg gatttacatc accagaggca atagcattcc ctataacttc
     8461 attgggagct gttggtgcag caatgtctct agttccagag tttataaagt caggagctat
     8521 aactcctaat gatatagcag tatttacagc tatgggtatg tgttggagtg gatatttaag
     8581 tacacatatt ggtatgatgg atgcattaaa tgctagacag ttggcaggta aagctattct
     8641 ttctcataca ataggtggtt tatgtgcagg agcagcagct cactttatat ttacactcgt
     8701 aggataaacg taatcagcta agataaaaaa taatatacaa aaaaatggtt gttgatatgt
     8761 aataagaatt tacatatcaa caaccatttt tttctgacta aagataattt ataatacata
     8821 aacattaagt ggatattaga cttaagttat atttatgtaa aaagtaacaa aaatctgtag
     8881 aaatttatga caaaatttag tacactaact atatgttctt atttaaaatt taaggttaga
     8941 attaggaatt acctagtaga gtaagctttg attaataaaa gaatcataaa ataaaaatat
     9001 tagtcataaa gattataaat aacttactta aaataaagcc atagaactta caaaaattaa
     9061 aaaagcatat aaatatggag gggtacaatg gatgaattat ttcatatagg aattgatgta
     9121 ggttctacaa cggtgaaggt tgtggtactt aataatagta ataatatagt tcataaagaa
     9181 tatagaagac attattctga tgtaaaaaaa tcagttaaag aagtactaaa tggtatatat
     9241 gaaaaattgg gagatataaa tactaccatt ataataactg gttcaggtgg aattggaata
     9301 tcaaaaaaat taggtgtaaa atttgttcag gaagttatat ctagtacaaa atcaatagag
     9361 tattttcatc ctgaaactga tgttgtcata gaacttgggg gtgaagatgc aaagataaca
     9421 tatctaagtg gaggaataga ccaaagaatg aatggaatat gtgcaggtgg cactggagca
     9481 ttcatagacc aaatggcatc acttttaaag acagatgcca gtggattaaa tgaacttgct
     9541 aaagggtata atgttatata tcctatagct tcaagatgtg gtgtttttgc taaaacagat
     9601 attcaacctc ttataaatga tggagccaat cagactgata tagcaatgag tattttcaat
     9661 gcagttgtcg tacaaactgt gagtgtcctt tcttgtggaa gaaaaataga aggaaatgtt
     9721 gcgtttttgg gtggtccatt gtacttttta tctgaactta gagaagcatt taaaagagta
     9781 ttggatttaa aagatgaaaa cataatcttc ccagaaaatg cacaacttta tattgctatt
     9841 ggagcaggat tattatcagt agatgaagat aatatacaac taaaatctct aatagaaaaa
     9901 ttggatagta taaagaatat tgaagatgga gaagtaaatt tattagaacc tttatttaaa
     9961 gataaaaatg agtatgaaga attttcaaga agacatgaaa aagaaaaaat aagttatgtg
    10021 gacataaatt caataaaaag caattgttat ttaggaattg atgcaggctc aactacaact
    10081 aaagcagcac ttattgatga ggatggaaga cttgtatatt cttattataa tagcaatgaa
    10141 ggaaatccat taaaaacaac cattaaagtt ataaatgaag tatatgatat attaccaaaa
    10201 aatattaaaa tattgagttc aactgtaact ggatatggag aaggtcttat taaaaaagct
    10261 cttaaaatcg ataacggaga gatagagact atagcacatt acaaagctgc taaatttttc
    10321 aataaagatg tagattttat acttgatatt ggtggtcaag atatgaagtg cctaaaaata
    10381 aaagatggag taattgataa tataatatta aatgaggctt gttcatcagg ttgtggctca
    10441 tttttagaga catttgcaag ttctttatct atgacaatag aagaatttgc acatgaaggt
    10501 atttattctc aaaatcctgt agatttaggt tcaagatgta ctgtatttat gaattctaga
    10561 gtaaaacaag ctcaaaaaga aggtgcaagt gttggagata tatcagcagg gctatcttat
    10621 tcagtaataa aaaatgcttt atttaaagta ataaagatta gagatttaaa tgagattggt
    10681 aataatattg ttgtacaagg tggaaccttt tataatgatt tggtgcttag aagctttgaa
    10741 aaactaattg gaaaaaatgt aattagacct aatatttcag gaattatggg agcttttggt
    10801 tcagcactta tagctaaaga aaaatatgaa agtggataca aaacatcctt gttatctaag
    10861 gaagagttga ataatatcaa gctagaagca agtgtaacta gatgtaaagg ttgttcaaat
    10921 cattgtcttc ttacaataaa taaattttct gataatgaaa tttttatttc aggtaataga
    10981 tgtgagaagg gtgaagctat ttatggtgat aaaaaaatag gattagaaaa gaaaaaacca
    11041 ataaatttat ttaagtacaa atacaataga atttttagat acaaaccatt ggaaaaagaa
    11101 gaagcaaaaa atggggaaat agggatacca agagtattaa atatgtatga agattatcca
    11161 ttttggttta cttttttcaa tgctatgggc tttagagttg tactttcaga taggtcatca
    11221 aaacagttat atgaaactgg tataactagt attgcatctg aaacagtttg ttatccagga
    11281 aaattagttc atggacatat agaaaattta attgcaaaag gtataaaaaa tatattttat
    11341 ccaagtgtaa caaatgaaaa taaagaggat tcaaatgcag ataattatta taattgtcct
    11401 gttgtaatat catattctga agtaataaaa aacaatgtag agaatataag aaataagaac
    11461 ataaattata taaatccatt tataagtcta aatgataagg aaaaattgaa gaaaagacta
    11521 tatgatgaat tatcaagaca tttttctgat ataaaaataa ctaaagaaga agttaatcat
    11581 gcagttgata aagctactga tgaacagaat ttatttaaaa tggagataca agcagcaggt
    11641 gaaagagcac ttaaagaaat aaaagaaaga aatatgaagg ggattgtgtt atgtggaaga
    11701 ccatatcata tagacccaga aataaatcat ggaatgccag agcttataaa ttcattagat
    11761 atggcagtgc ttactgaaga ctctatatgt catttagcca atgtacaaag accacttaga
    11821 gttgtagacc aatgggtata tcattcaaga ttgtataaag cagctagttt tgtaagagat
    11881 aaaaactatt tggaacttgt tcaacttaat tcatttggct gtggattaga tgcagtaact
    11941 acagaccagg ttcaagaaat acttaatgaa aaatcgaaga tatatactat tataaagatt
    12001 gacgaaggca acaatttagg agcagctaag ataaggatga ggtccttaaa agcagctatg
    12061 tttgagagag aaagcaaaaa tatagatgta aaaaatataa aggtaaacag gatacagtat
    12121 gctaaaaata atcagataac gaacaaacat actatattag caccacaaat gtctccaata
    12181 catttccaat tccttgaaaa agctgttaac ttaagtggtt ataatataga aattctaaaa
    12241 gatactgata gtagtgtaat tgaagaaggg ttaagatatg taaataatga tgcatgttat
    12301 ccagctataa tagttgtagg tcagctaata acagcattaa aatctggcaa atatgattta
    12361 aataatactt ctgttactat aactcagact ggtggaggtt gtagagctac aaattatatt
    12421 ggattcttaa gaaaggctat atatgatgct ggatttaaag atgttcctgt cattgcactt
    12481 agtgtaaatg gaatagaaga tagtggtata atggataata tatcactaaa attaatcaat
    12541 agactcttta tgtctgttgt ttatggtgat ttgctgatga aagtactata tagagtaaga
    12601 ccatatgaaa aagttactgg aagtgcaaat gctctatatg aaaaatgggt tgatatatgc
    12661 aaatcttctc ttgttaaagc aaaaatttct gtatttacta gaaatataaa agatatagta
    12721 aaagaatttg ataatttaga gatactagat atcaaaaaac caaaagtagg tttggttgga
    12781 gaaatattgg tgaaattcca tccaattgca aataataatt tagtagatat attagagcga
    12841 gaaggtgctg aagcagtcgt gccagacctt acaaatttct tcttaagttg tgcctttaat
    12901 actatataca aacacactca tttagaagga agtagaaaga gtagaatgat aggagaagca
    12961 tttatataca taactggaat atatcaaaga gtatataaaa aagcacttga taagagtgag
    13021 agattttatg ctccagccaa tataaaaaat gttgctaaaa gtacagaacc agtagtgtca
    13081 ttaggtaatc aaactggaga aggttggctt ttaacaggtg agatggttga attattaaat
    13141 gaaggtgtag aaaacattat ttgtatgcaa ccttttggat gtttacctaa tcacataatt
    13201 ggtaagggtt ctataaaaga attaaagagg ttgtataaaa atgcaaatat aatacctatt
    13261 gattatgacc cttctgcaag tgaggtaaat caacttaaca ggataaaact tatgctttct
    13321 aaggctttta aaaatatata agatttatat tttgatatgt taaaagttaa tgaatttaaa
    13381 aatatactct atattaagta tctataaaaa agagttatcc tattttttag ggtaactctt
    13441 tattagttaa aaaattatat gtatactgat attaaagggg ggaagtaata tgagtttagt
    13501 atatgatgtt tataaatcaa aattaggaaa tttgtacata ttaagtgaag atggttttat
    13561 agttagtgta tacataggag atgaaaaatt taataattta aaaaatgaat gtattaaaaa
    13621 aggaattgat ataaagataa gtaaggaaga tttaaaggaa gcaataagcc agatagatga
    13681 ttactttaat ggaaaaagaa aagtctttaa tttaaatatt cgtattgaag ggactgaatt
    13741 tcaaaaatca gtttataggg aaatgttaaa aataccttat ggagaaacat tgtgttattc
    13801 agatatagca agaaatataa acaatccaaa atcagtaaga gcaataggac aagcaagtaa
    13861 aagtaataaa attccaataa taataccttg tcatagagta gttggcaaaa aaaatattgg
    13921 tggatatatg gggaatcatt ctgatttgaa agagatacta ttaaatttag aaagacagag
    13981 ttaacttaga aagaaatgtt tttattgtta atatttgata taataatagt aaaaagaagt
    14041 attttttaca aaaacaatga agaatttatt ttaatttaca aaaatgaggg ggaattttaa
    14101 tgtgtgataa agttatagca tcaaaatatc atgagaaggg atttaattgt gcagaatcag
    14161 ttataaaagc atataatgag gaatttaata cagatatacc agtttgttta ggaagtggac
    14221 tgggttcagg gtgtggggtt gctagtttat gtggtgcagt aaatgcatca aatataataa
    14281 ttggatacgt aaaaggcaga aaccacgaag atgaaagtac taaagctaaa gtatatgcta
    14341 aagatttaac tacaacagta agaaaagagt atggttcaga gttatgtata gacctaaaaa
    14401 aagatttagt tgcttgtaga gaaataatgg atttcgcata tgatagtcta aaggaaactt
    14461 taaaaaaaga attataagaa aataagctat caagtataaa ttatttgata gcttattttt
    14521 atagtcgata ctaaataaat caaatatatt ttagaattat tatttgtaaa taaacagcaa
    14581 ataattttag gatttttatt taatatagtt t
//
LOCUS       DAELSY010000100        14480 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.100, whole genome shotgun sequence.
ACCESSION   DAELSY010000100 DAELSY010000000
VERSION     DAELSY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14480
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.100"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            82..996
                     /locus_tag="KPG09_002625"
     CDS             82..996
                     /locus_tag="KPG09_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0967222.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            1038..2210
                     /locus_tag="KPG09_002626"
     CDS             1038..2210
                     /locus_tag="KPG09_002626"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBG0967223.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            2257..3069
                     /locus_tag="KPG09_002627"
     CDS             2257..3069
                     /locus_tag="KPG09_002627"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBG0967224.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            3273..4598
                     /locus_tag="KPG09_002628"
     CDS             3273..4598
                     /locus_tag="KPG09_002628"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBG0967225.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEVDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSIKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            4811..5890
                     /gene="mltG"
                     /locus_tag="KPG09_002629"
     CDS             4811..5890
                     /gene="mltG"
                     /locus_tag="KPG09_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBG0967226.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            6062..6736
                     /locus_tag="KPG09_002630"
     CDS             6062..6736
                     /locus_tag="KPG09_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBG0967227.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFASIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            6723..7970
                     /locus_tag="KPG09_002631"
     CDS             6723..7970
                     /locus_tag="KPG09_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG0967228.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRAPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            8042..9706
                     /locus_tag="KPG09_002632"
     CDS             8042..9706
                     /locus_tag="KPG09_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBG0967229.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(9803..10087)
                     /locus_tag="KPG09_002633"
     CDS             complement(9803..10087)
                     /locus_tag="KPG09_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0967230.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(10110..11627)
                     /locus_tag="KPG09_002634"
     CDS             complement(10110..11627)
                     /locus_tag="KPG09_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG0967231.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKACDLDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAIYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            complement(11752..12573)
                     /locus_tag="KPG09_002635"
     CDS             complement(11752..12573)
                     /locus_tag="KPG09_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0967232.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIEIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETK
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKSLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            12764..14191
                     /locus_tag="KPG09_002636"
     CDS             12764..14191
                     /locus_tag="KPG09_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HBG0967233.1"
                     /translation="MKIKKLLTIGLSLSIFIASCPISANALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMADGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSINKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKLVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTIAKNNAIVLVGNDSNKSV
                     LKSTIKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYE
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKIYN"
     gene            14334..>14480
                     /locus_tag="KPG09_002637"
     CDS             14334..>14480
                     /locus_tag="KPG09_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967234.1"
                     /translation="MDNFLFNVLASLTASVVVYLISKLFKKAKSHSRMNYTPLRRQLK
                     YVKLS"
BASE COUNT         5586 a         1652 c         2461 g         4781 t
ORIGIN      
        1 actatcattg gggttgcaat taatattaaa tactgtaagt attggtatta ttaagttcct
       61 agctaaataa aggagtttat aatggatatt atagagggat atatagatta cataaaagat
      121 aaaaaaaaat tatcagagaa cacagtggca tcatacttta tggatataaa aaaatatatg
      181 gaatatataa atcaaaaaag cattaaacta gtagatatag tagaaaatga tattataagt
      241 tacttaataa agttggaaaa agataatgta tcaatagcta caatagcgag gatgatatcc
      301 tctataaaat cttttcatga ttatttattt ttaaatcata tatgtacaaa taatcctgca
      361 aaggatataa aaaaacctaa aataaaaaaa gaaaatataa atatacttac agaagaagaa
      421 attgaaagac tattaaattt tccaaagtta acaacaccta aattgattag ggacaaggct
      481 atttttgaag tattatatgg tactggtata aaagtatctg aattagtaga gatgaatata
      541 gaggatattg acctagatat tgattatata tattgtaatt catgttgtaa aaatcaaaga
      601 gtgataccac tttgtgatat tacaaaactt tacttagaaa agtatctaaa agaagcaaga
      661 cctaaaatgg ctgtagaagg tgaaaaatca ttattcgtaa gttcattagg tcaaaggttt
      721 acacgacaag gtctttggaa agtcataaaa aaatattcta atctagccaa tatagataaa
      781 aatataaatc ctacaatgct tagacattca tttgctattc atttgttaaa tgaaggtgct
      841 aacattgctg ttgtaagtaa aattttagga aatgttaatt tatctagttt gcaagtatat
      901 ttaaatcata tagataaaaa cgtgagaaga gaaataaagg aaaaacatcc tagaaatgat
      961 gttgatgtag aattacaaaa agcagaagct aaatagacta tatagcaaat tttataattt
     1021 aaaaggagta tatttatatg agcagagtaa tttggattgt aattgatagt gttgggatag
     1081 gagcattacc tgatgctgaa aaatttggtg atagtaagga tgtaagtacc cttggaaata
     1141 tatttaagga atatccagat attcagattc ctaatatgag aaatttaggt ataggaaaca
     1201 tagatggtat agattttttt gaaagtataa aagaaccaat aggttgtttt ggaaagtgta
     1261 aggaaatgtc tcagggaaaa gatactacta caggtcattg ggaaatgact ggtataatag
     1321 ttgacaaacc atttaagaca tttgaacatg gattctctaa ggagataata gaagagtttg
     1381 agaaaaaaac tggaagaaaa gtagtaggaa ataaaccagc ttcaggaact gtaataatag
     1441 atgagtatgg agagcatcaa ataaaaactg gagatgtaat agtatataca tcagctgata
     1501 gtgtatttca gattgctgct aatgaagaag taattcccct agaagaactc tataatatgt
     1561 gcaaaatcgc tagggaaata atgatggggg ataatgcagt agctagagta attgctagac
     1621 cattcatagg taagaaaaaa ggtgagtttg tgaggacttc aaatagaaga gattattcac
     1681 tagacccatt tgaaccaaca gttcttgata atataaaaga atcaggactt gatgttttag
     1741 cagttggaaa aatagaagat atttttaatg gaaaaggaat tacagatgct atccacacta
     1801 aaagcaatat ggatggagtg gatgaaactt taaattatat gaaacaagat aataaaggtc
     1861 taatatattc aaatctcgtt gattttgatt ctaaatatgg tcatagaaga gacccagaag
     1921 gatataagaa agctcttgaa gagtttgata gtagacttcc tgaaataatg gctaatatga
     1981 gagaagatga tattttaata atcaatgcag accatggaaa tgacccaaca tataaaggta
     2041 cagaccatac aagagaatat atacctgtta tgatttatgg caataagatt aaaaaaggtt
     2101 ttaatttagg agtaaaagat acttttgctg atattggagc aactgttgca gatatactaa
     2161 atgtaaagtt gcctaaacat gggtcaagct ttaaagaaga tttattttaa actataaaat
     2221 gataaaaact tagcttaata ggatggagag attggaatgt ttgaaaagat agcacaaagt
     2281 agtaaattta taaattctaa aagtaatata aaacctaaga ttggtttgat attaggttct
     2341 ggactaggtg atttagcaaa tgatatagaa gaccctgtca caattaaata tagtgaaatt
     2401 ccaaacttcc ctgtatcaac agtagcagga catgctggac aacttgtaat tggaaaactt
     2461 gaaggaaaag aagttattgc tatgcaaggt agatttcatt actatgaagg atattctcag
     2521 aaagaagcta cttttccagt aagggttatg aaagaattag gagtcgagat tcttatagta
     2581 actaacgctg ctggaggcgt aaataaagaa tttaaagcag gagatttgat gcttataaga
     2641 gaccatataa actttagtgg aagtaatcca ttagtaggaa aaaatgatga cagatttggt
     2701 gctagatttc cagatatgtc agatgcatat tcttctaaat atgtagatgt agttaaaaac
     2761 tgcgctaaag aatgtaatat agatgtcaaa gaaggcgttt atatgttttt tagtggtcca
     2821 aactatgaaa caccagcaga agtgagaatg gcacaaattt tgggagctga tgctgtaggt
     2881 atgtctactg ttccagaagt catagtagct tctcactcca atataggagt tattggtata
     2941 tcttgtataa caaatatggc tgctggcatc ttaaatcaac cactaagtca tgaggaagta
     3001 atagaaacta ctcaaaaagt taaatcagaa tttatgaatt tagttaaaac aacggttaaa
     3061 aatttataag ttaaagctta ttatatattt tttgaggaag tttataataa aatagttaat
     3121 gtttaatttg aaaagaatta ataaaaagct gtcttgatat agaaagttat ttctaataaa
     3181 ggacagtttt tttataatac tacaacattt gaaagtgttt taaagtttaa tatttattta
     3241 aattattata gattagaatg gagatttcaa ttatgagaat gtatgatatt ataaaaaaaa
     3301 agagagataa cttagagctt agtaaaaaag agataaacta ctttatagaa aactattcaa
     3361 agggagatat acctgactat caagcttctg cactattaat gtcgatttat ttaaataaga
     3421 tgaataaaca ggaaactgta catcttacag aagcaatgat gaactctgga gacatgataa
     3481 acctgtctga ggtagatgga ataaaggtag ataagcatag tactggtggt gttggagata
     3541 agactaccat agctcttata cctttggttg cttcatgtgg agcaccagtt gcaaagatgt
     3601 caggtagagg tcttggtcat actggtggta ctattgataa attggagtct ataccaggtt
     3661 tttcgacaga aatggagata agtaaattta ttgattctgt aaataagtct aaaatagcag
     3721 tttgtggtca aagtgcaaag gtagcaattg cagataagaa aatatatgct cttagagatg
     3781 ttacagcaac tgttgataat atatcattga tatcatcaag tgtaatgtct aaaaagttag
     3841 cttcaggagc tgatgcaatt gtgttagatg taaaaactgg tgatggagca tttatgaaga
     3901 cattagaaga gtcttttgag ttagctaaat ctatggttga tataggtact ggtatgaata
     3961 gagatacaat agggattgta acagatatgg atgaaccttt aggttttgca gtgggaaact
     4021 ctttagaagt tatagaagca attgagacat taaaaggaaa tggccctaaa gaccttgttg
     4081 agctatgtga agtattagga gcatatatgt tggttttatc aaaggtagca catgattttg
     4141 aagatggtgt tgaaaagata agagaatcaa taaaatctgg tactgctata caaaaactca
     4201 aagagtttat agagaatcaa ggtggtaata aagatgtagt agatgattat tctttattca
     4261 aaaaatcaaa gtataaatat ccagttaaat caattaaatc aggttatata agtaaaatta
     4321 aagctgaaga tgttggtgta tcagctatga ttcttggagc aggtagagaa actaaagatg
     4381 atattttaga tttatcagca gggataattt tagaaaagaa agtaggagat tatgtaaatg
     4441 aaggtgaagt tttagcttac atgtattata atgatgaaca aaagctttca ttggcaaagg
     4501 gaagatttat tgattcctac tctattgtag attcaaaaat agaaaaaaat aaattaatat
     4561 atggtattgt aactaaagaa gagattaaaa agttttagaa aattttagaa aagtgattat
     4621 aaaaggccta acatgtcaaa aataatgtta ggttttttat tttttgataa actataaatt
     4681 ataccaaaaa tatatatttt aaaacatttt ttacaatata aataagttta ttctacaaga
     4741 tgattgaaaa ttaaagtatc tagttttata atagaaaatg gataaatata cataaggagt
     4801 agattatatt atgaacttta aagaaaatag attgaaaata gcagtattaa taattgttat
     4861 tttgattatc ttagcaggta tatttgtgtt tatacaaata ggtccttatg ataaaaataa
     4921 caaaaaagat gtaattattg acgttccaag tggagcatct gttggaaaaa tatctgacat
     4981 attatatgaa aataaattaa taaagaatga actattattt aaacttttgg taaaggttag
     5041 caataaagct ccgtctataa aatcaggaac atacttactt aatcaatcgt attcaaacaa
     5101 tgatataata agtcttttag tttcaggtaa aatatatcag gatggtataa aagtgactat
     5161 tccagaggga gctacatcta aagaaattat agcaatgtta gtaagtaaaa atttaggaga
     5221 taaagctact tttgaaaatt taataaaaaa acctcaagaa ttttatgata aatttccata
     5281 tttgaaggaa gatggaataa cctctttaga agggttcttg tatccagaaa cttattattt
     5341 taattcaaag aaacaatcag aagaagatat tttaagcgaa atgttaaaag tatttgacag
     5401 taaatacacg gacaagttta aaaagaaaca aaaagaatta aatatgactc ttcaagaagt
     5461 aatggaaatg gcatcaataa ttgagaaaga agctgttctt gacaaagaca gacctataat
     5521 agcaagtgtg ttttataata gacttaaggt aggtatgcct cttcaatcag atgctacaat
     5581 acaatatata tttgaagaga gaaagaaaat tgtaacatat gatgatttaa aaattgattc
     5641 accatataat agttataaaa ataagggatt acctcctaca ccaatatcaa atcctggtat
     5701 aaaatctatt gaagcagcat tatatccaga gaaaacagac tatttatatt ttgtagctaa
     5761 gattgatggt ggaaataatt atagtacaaa ctatcaagac catttaaaat acgttaaaga
     5821 atataaagaa gcaagggata aacaatcaaa agatacaaaa gcaacaaata aagaaaatac
     5881 aaaaaaataa accatgatta taaaaagtct aacagggtaa gaataatgtt gggctttttt
     5941 attgaaaatt gttattctta gatttataat aaagaaagtg taaatatata taacaatatg
     6001 aatttaaaaa ataataaatt aatataaaac ttgaatattt attattttag ggagtgtttt
     6061 tatgagtaat atagttaatg atttggttga agattatata agagtgacat taaaagaaaa
     6121 agaaggattt ttaaaagatt tagaaattta tgcagaggaa aatagtgttc ctataataca
     6181 taaagaagtt tcagatttat taaaagtctt gttaaaggtt caaaaaccaa agagaatttt
     6241 agaagtagga tgtgcaattg gttattcttc tatttttttt gcaagtatta taggaaaaga
     6301 tgcagatata ataactgttg agagaaatga aaaaatgata gaaaaagcaa aagaaaacat
     6361 aaaattagct ggatttgaca ataacataac tatactagaa ggagatgcag aagaaaaact
     6421 tagtcaagtc caaggtgaat ttgaccttat atttatagat gcagccaaag gtcaatataa
     6481 gctatttttt gatttagtta tagataaatt aaaagatggg ggacttttag tatctgataa
     6541 tattttgtat aaaggtatgg ttgctcatga tgattttgtt gtaagaagaa aaaagactat
     6601 agttaaaaga atgagaaatt atttagatta tatatgtaat tgtgactatt taagcacatc
     6661 attggtacca ataggtgatg gtgtagcact aagttataaa gaaagtaaaa gaggtgatgt
     6721 agatggaatt aaataaagta gaacttttag ctccagcagg tgatttggag agattgaaaa
     6781 tagctataac atatggagca gatgccgttt atataggtgg agaaattttt ggaatgaggt
     6841 ctgcagcgaa gaattttagt aaggaagata tggcagaggg agtagctttt gcacatgaaa
     6901 gaggcaagaa agtattcgta acagtaaata taatacctca taatgaagat tttctacaat
     6961 tagaagacta tttgttggaa ctagaagaga taggaataga tgcagttata atagctgacc
     7021 caggagtgct tagtgtcatt aaaaaagtaa ttccgaatat ggaaatacat ttaagtacac
     7081 aagcaaatac aaccaattat ctaagtgcaa acttctggta cgagcatgga ataaaaagag
     7141 ttgtagtggc aagagaatta tcttttgatg aaatatctga gataagagca aaaacacctt
     7201 tagatatgga tatagaagcg tttatgcatg gtgctatgtg tatttcttat tctggtagat
     7261 gtttaataag caactatatg actggaagag atgcaaacaa aggttcttgt gctcaatctt
     7321 gtagatggca atatcatttg gtggaagaaa aaagacctgg tgagtatttt ccaatatacg
     7381 aagatgaaag gggtacattc ttctttaact caaaagattt atgtatgata gagtatatac
     7441 cagagttaat caaatctggt ataacaagtt taaaaataga aggtagaatg aagactgcat
     7501 actatgtagc tactgttgta agagcataca gaatggcaat agatgaattt tacagagccc
     7561 cagaaaactg gaagttcaat ccaatgtgga tggaagaact aaaaaaaggt agtcatagac
     7621 attttacatc aggtttttac ttgaataaac caacaacaga agaccaaaat tatcaatcag
     7681 catcatatgt tagaaactat gattttatag gtatagttag agaaacagaa gatgaagatg
     7741 gtcttattgt tgttgagcaa agaaataaaa tgtgtgttgg tgatgaaata gaagttatgg
     7801 gaccatataa agaaactatg tttactaaaa ttgaagaaat gtacaatgaa gaaggagaag
     7861 ctatagaatc agctcctcat cctagacaaa tagtgaagtt aaagctatca gtaaaagttg
     7921 gtaaagatta tatgcttaga aaagttatag aagaaaaggt tgaagaataa taaaaaattg
     7981 ataaaaatag tattagcccc ttagaaattg tcaataatga aattatctaa ggggtgattt
     8041 catgtcaaaa aagaagactc catttttgaa aaaagtggga aagagaagct ggtgtatatt
     8101 tactataatt ttaataattt atagtgtttt aatttatagg ctagttgata tacaagtatt
     8161 aaaaggtgat aaatacaaac aaagtgttga atcacaaagc gttgagaaag tcgagttaaa
     8221 tagtggaaga ggaataatct atgatagaaa caacaagaag cttacagata caagtaaatc
     8281 tcaggtatta attgttgaaa aagaaaaatt aaataataac tataaaattt tggaacttat
     8341 taagaaagct actaagatga atgatttaga tatatataaa gctgtacaag agcaattgac
     8401 acgccctata atacagattc agactaaaaa tatcgataag agtatgaaaa aagaacttga
     8461 gaaaaatgga ataatggtag aagaaaaaac tatgaggtac gcaaaagatg gtttattatc
     8521 acatacaata ggatatataa aagaagatga taaatcaggt caatcaggaa ttgaaaagag
     8581 tatggatagt gtacttagaa attcaaatga aaagtatata agtgctttta aagctggaga
     8641 tgcaggaaat gaaaaatctc taaacatact aaagggaagt gtaaaaactg ttgataataa
     8701 ggataaagat agacatctaa aaactactat tgattataac attcaaaaaa aattagaaca
     8761 aatactaaat aaagaagaaa atcctacagc agcaataatc tcggaagcat ctacaggtga
     8821 aattcttgca atgtgctcta gacctaattt tgaccaaaat gacatatcaa aaagtttaaa
     8881 aggaaaaaat ggggaatttg aaaatagagt tataaaagcg acatatccac cagggtctgt
     8941 atttaagatg gtagttttat tttctgcact tgaaaatggt gttatagatg agaactatac
     9001 ttacaactgt acaggtaaga ctaaagttgg gaatactaat gaaatactgc gatgtaataa
     9061 aagagatggt catggtttcc aaaacttaag acaagctttt tctaattcat gcaatcctgc
     9121 atttttagat atagctatga aacttggaaa agaaaagata ctcaaatctg ctgaaaaatt
     9181 acatttattt gaaaaggttg atattggtct tgatgaagaa aaaattagag aagctccaaa
     9241 aaacatatca ataagaaatt tagctatagg tcaagaaaat atagaattta caccacttca
     9301 aataaatcaa atgactcaaa taatagctaa taatggaaca ttcaaaccac tatacttata
     9361 taaaagtctt gtggacaata atatgaatac aataaaaact tataagtctt caaaaaaaga
     9421 agaacttatt tctccttatg tgtgtacaca agttaaagaa tatatgaagt ctgtatctag
     9481 gattggtaca gctaaagacc taaaagatat tgaaggtggt tgtggagtta aaactggtac
     9541 agctcaaagt agtttaaata aaaaagctat agaccacgga tggataacag gattttatcc
     9601 tgaagaaaga cctaagtatg taataactgt gttagtagaa ggaactcaaa aaggaaataa
     9661 atctgcaaca cctatattta aggaaatatg tgaaagtata aaataaatta tgtactataa
     9721 aatttaagga tatatcgtaa ctttgatttt agatagaaaa ctttgtctaa tgatgtacga
     9781 tatatccttt taaaatatag tattaacttt cttgaacaag ctctttttct agttttttaa
     9841 gagctttttt ttcgattcta gatacatatg accttgatat atcgagtttt tgtgcaattt
     9901 ctctttgtgt cttatagcca taaggagtta acccatatct taactggaca atttctcttt
     9961 ctctaggagt aagaattttg tttagttgtt cgtaaagttt tccaacttga acttttagtt
    10021 caacttcatc aagaacatag ttagcctctg tgccaagtat atctaaaaga cttatttcat
    10081 tacacactac tctttatatg ttattggtat tactggtctt catgtaattt caaatgaacc
    10141 attaattcaa aagaatctag tttttgttct ttagacttat aataaattgc tttatcaatt
    10201 acagatttta ggagtttatt ttttccaagg gaatctgaat catgataaag tgataacaca
    10261 gttttaatat cttctattat tttagaattg tcttttttaa cagtactcaa tctatttttt
    10321 actgtcttaa ttgaatcttt aatttcttca gttcttgaac ttatattgtt ggacctttca
    10381 ataaatgtat cttcatcata tattccacgt tctaataaat catataactt atttttttgt
    10441 ttacctaatt cttttaattc agtttctaag ccctttaaga ctcgtttttg aaattctata
    10501 ttactatctt tattattttt atctaagtct aaatcacatg cttttaattg atattttttt
    10561 accgtgtctt ccaaagattt taaaatggca gcttcaataa attcaaatcg agaactttta
    10621 ttgcaaccag gatggtaaca aattatatag tcgtggtctg catatggtct atatactaat
    10681 ggtctaccac aatttttgca aatcactatt cctgcaagtg gattttttat ttgattacca
    10741 tagctgacat gaccgttttt tttaagtata ttttgagctt ttttccatgt tatttcactt
    10801 attaaaggtt catgctttcc ttttgcttca atccattcat ctttaggtct tagcttaaca
    10861 gtttttattt tatttggatt tttagatttt ttatagtcct ttttttgcca ctgaatgtag
    10921 ccacaatata ccttattttt tattatattt gtaattgcag aattgtacca attagcacct
    10981 gtagcagttt taatgcctaa gttatttagg tattttgata ttttactaca acccatatct
    11041 tcattaatat ataagtcaaa gatagtccta actacatcag catccttatt tggtacaagt
    11101 attctttctt ttctacctgt tgttacagca tcatatccaa atggagcaga agttcctatg
    11161 aaattccctt cttcaactga ttttattcta cctctttgca ttcttcttga tataagtttt
    11221 aattcttttc ttgccataaa tgcttcaaat tctgaatact cctcatcaaa ttcatctgtt
    11281 aggtcgtatg tttttctagg tgtgatgatt ttggttttac tttctttgaa tgtttctaat
    11341 atgatacctt ggtctttcat gtctccacga ccaagtctgt ctaagtccat tactaagact
    11401 gcatcatatt tattttcttc aacttctttt agaagttcaa gcattttggg tctatgtatt
    11461 atactttcac cagaaactaa ttcttctttt atctcaatta catctaagtt ttgttctttt
    11521 gctagtttta gtagggttga tttatgtcta cttaaagttt caaattcgcc ttgtttttct
    11581 gcctcttcat cagcacggga ttttcttaag tatatggcaa cactcataat aagtaatcac
    11641 actccttatg cactattata tagtaaatca atagagttta aaatatagaa aataagcatg
    11701 tcaaaagtta atttaacttt caacatactt attttctata atattaatgc cttataatcc
    11761 tttaatatca tttagattag tttttgcata ttcattatcc gggttaagtt caagcgcctt
    11821 attgtaagat tctctagctt tattaaattc tgatttactc ataaaaataa cacctcttaa
    11881 attgtaagca tcactagcgt ctattttata agcttcatta tttttatcta gatattctaa
    11941 ggatttatca attgacttta aagcctcatc atatcgttgt aactctatat aaatatatga
    12001 ttgtgtataa tatgcaagtg gatttttatc atcaatttct atagcttttt tgatatcaat
    12061 agcagcttta ttataatcct tatcttcatc tttttttgtt tctgacctag cgattttgac
    12121 ataattagag ctaagagctc ttttaaagtc tttatgccat aataatttat attcattatc
    12181 ttcgattata aaatatctat caaaatttgc agtatcttct ttattttcag tgatatactt
    12241 tatagtatca gtttctttta ttttaacaag atttgtgtac tctttattag aatatttgta
    12301 tttctctact ttattttctt caccaagttt ataatctttt agggtttgaa gtttaaataa
    12361 agtatcttga tattcttcaa atttttctag actgacactt tccttactgt ctttacttaa
    12421 taaattatat gctacttcgt agtctttgat ttctatacta tcaaggtact ttttagctac
    12481 ttctgtagca ctttcttttg gagcacatcc aaccattgat atcataatta aaaatgatac
    12541 taatattgct gttatttttt tactgattat cataatataa tcccccttat attgtctctc
    12601 actaatttta ttttatcacc atattctacc aaagtcatta atttaagaat gatatttttc
    12661 attaatatta tattaagtaa taataatttt taaaatttat ggtaacattt tcctgaaaag
    12721 tattatataa tttaggtgaa aatatttaag agaaggagat attatgaaaa ttaaaaaatt
    12781 attaacaata gggttatctt tatctatatt tattgctagt tgccctatat ctgcaaatgc
    12841 tcttgataaa attgaatcaa ttaaaggtgc agataaatac gaaactgctg gtataattgc
    12901 agacaaacaa aactatacta cagctatatt aattaatgca gacagtacaa tggctgatgg
    12961 attaagtgca agtggacttg caggagctat aaacgctcca attcttttga ctaagaaaaa
    13021 taacatacca aatgctacac ttaaaagatt agaaaaggca aaaaaagtat atataattgg
    13081 aggagaaaac tctattaata aatatactga aactgttcta aaaggcaaag gaatagagat
    13141 taaaagactt caaggtagtg atagaattaa gacaagctat aatgttgcaa aagaaataaa
    13201 ttcaattaac aaagtaaata aagtcatatt gactaatgct tttaaaggag agcctgatgc
    13261 aatgagtgct gcacctgtag cagttagaga taaagcagct atagtattaa ctgatggaaa
    13321 aagtgttcca tttaatacaa ctggtgtaga aagttatgta ataggtggta cttcatcaat
    13381 gaatgacaaa ctagttaagg atactaattc aactagatta ggtgggacag atagatatga
    13441 tactaacaaa aagataataa ataaatttta tagtggagta aaagaatttt atatagcaag
    13501 tggtactgat ttagtgtatg ctcttgttgg ttctaccatt gctaaaaata atgctattgt
    13561 gttagttggt aatgatagta ataaaagtgt attgaaaagt acaattaaat taactgctat
    13621 aggaaatttg agtgatagta tattacagca gtgtttaaat gtaacaaaaa atataggcga
    13681 ttctaatact ggaagagaat atacagtaga agatgctgct aatgcagtga aaaaactatt
    13741 aggtgtgaag aaattttatt acgaacctaa ttattcagaa gatgaagatt taatatttct
    13801 actagataaa aattttgatt tttcaggaaa agagtactat gtatttgctt acattaaaca
    13861 gggtgcagag ggtgatgtta gatactgtgt agctaaaaat gatatgagca aagtttatac
    13921 atatgatgtt aatggtaata tgaaagtatg taatccagat gattataaaa ataaaggaag
    13981 tataacagag gaacaagcaa aacagatagc attaaaagaa tgtgctaaga gacataaagt
    14041 cagtatcaat aacttagtag ttgatatgat agattttaga gatgatatat atagtggtaa
    14101 tgtctatgct gttaaaatca attcaaaaaa tagtaatggt acttttggtt ggttttttat
    14161 agatatagaa actgggaaaa tttataatta aaattagaac ttttaaacaa taattatata
    14221 atttaccata atatgttata atatttttag caagaagatt taacttacaa tctatagagt
    14281 ggagttcatg tacaaaagat tatcctccca acgattcgaa gggaggtgag ttcatggata
    14341 actttttgtt taatgtttta gctagtttaa cagctagtgt ggtagtttac ttaatcagta
    14401 aactattcaa aaaagcaaaa agccactctc gcatgaacta tacccctctt aggagacagt
    14461 tgaaatatgt aaaactgtct
//
LOCUS       DAELSY010000101        14255 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.101, whole genome shotgun sequence.
ACCESSION   DAELSY010000101 DAELSY010000000
VERSION     DAELSY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14255
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.101"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      complement(351..434)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(667..1509)
                     /locus_tag="KPG09_002638"
     CDS             complement(667..1509)
                     /locus_tag="KPG09_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0967235.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVMANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(1761..3545)
                     /gene="pepF"
                     /locus_tag="KPG09_002639"
     CDS             complement(1761..3545)
                     /gene="pepF"
                     /locus_tag="KPG09_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG0967236.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEKYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(3611..4276)
                     /locus_tag="KPG09_002640"
     CDS             complement(3611..4276)
                     /locus_tag="KPG09_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG0967237.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(4427..4588)
                     /locus_tag="KPG09_002641"
     CDS             complement(4427..4588)
                     /locus_tag="KPG09_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBG0967238.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(4666..5175)
                     /locus_tag="KPG09_002642"
     CDS             complement(4666..5175)
                     /locus_tag="KPG09_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBG0967239.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            5381..5599
                     /locus_tag="KPG09_002643"
     CDS             5381..5599
                     /locus_tag="KPG09_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0967240.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            5617..7563
                     /gene="feoB"
                     /locus_tag="KPG09_002644"
     CDS             5617..7563
                     /gene="feoB"
                     /locus_tag="KPG09_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG0967241.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKSNTH"
     gene            complement(7723..8778)
                     /locus_tag="KPG09_002645"
     CDS             complement(7723..8778)
                     /locus_tag="KPG09_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0967242.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRANLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGTISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(8826..9626)
                     /locus_tag="KPG09_002646"
     CDS             complement(8826..9626)
                     /locus_tag="KPG09_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0967243.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     KVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFLIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(9619..10410)
                     /locus_tag="KPG09_002647"
     CDS             complement(9619..10410)
                     /locus_tag="KPG09_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0967244.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMFDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTTLINAIPKVILDGLGVAGTLLPAVGFALLMDMLISKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAMNTLTEGEIDFE"
     gene            complement(10450..10872)
                     /locus_tag="KPG09_002648"
     CDS             complement(10450..10872)
                     /locus_tag="KPG09_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBG0967245.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLANIEYFCMTKDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSIFL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENRNKIKNVM"
     gene            complement(10888..11367)
                     /locus_tag="KPG09_002649"
     CDS             complement(10888..11367)
                     /locus_tag="KPG09_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0967246.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTRDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKEIGDLGIDIELRMLPRDKKKQLSDLIK"
     gene            complement(11499..14255)
                     /locus_tag="KPG09_002650"
     CDS             complement(11499..14255)
                     /locus_tag="KPG09_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0967247.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSKFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNTILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEIFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALESKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINGPMLFIDQISTISALEVGNLLMQGKNIEQIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLPNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         4608 a         2470 c         1622 g         5555 t
ORIGIN      
        1 agtcattttc acttataaat ttattatttt tctagaaact tgtctattgg ctagtttgag
       61 tattgattta tatgatttat atggttataa tataaaataa attgtattat tttggtaaat
      121 atattgttac aaaaggttta tattttgcat ctcaatgttt ataaaaactt tattttctta
      181 ttataatagt attatacttt ttaaaaaatt tcccttttcc aacaaatggc tactttaaaa
      241 gccaaaattt cacctatcaa taaaaatctt aacgactaat actcttattt tcaaattttg
      301 caacattttt gttaatttta aagcaaaaaa agactatgca atataaaaat tatattcata
      361 gccggttgca ccactaactc catatatctc cagatgccca ataataagat atacttctca
      421 gtttctaaaa tatttttatt aaaaatcttt tatatatttt cataaatttc ttttttattc
      481 taacattgta atattatttt gtcaatattt ccatattttt agatataaga tactaaaata
      541 cctattttat ctaaagatat ttatatttct acattcaata atagacaata ccctgcaagt
      601 taaagcatca tttttatgag cttatctcac agggtattat atgtttactt tatattataa
      661 ttcatgttat tgattaactt taataaatcc tttttctgaa acaagtgctt gtccttcatc
      721 acttaatata taatctatta aagctttccc tttttctgac aaaccatctt ctttattaac
      781 taatataaat ggtcttgata ttggatattt attagccatt acatttgcat cagttgcttc
      841 aacatcattt atttttaaac tatttacttt ctcatcaaca tatccaaatg atatatatcc
      901 tatagcattt tcatttcctt caactgtagt ttttatattt cctgaaccat cacttatttg
      961 tgcatcttta gttagttctt ctgaatcaaa ccctacaatt tcttggaatc catctctagt
     1021 tcctgaacct tcttctcttg atactattac tattggtgca tcatttcctc ctacttcttt
     1081 ccagttagta atctttcctg tatatatacc tttaacttgg tccattgtca ggttttttac
     1141 tgagtttgca gtattagtta tcaatgctat accatctatt gctattttag tttcctttaa
     1201 tccagcactt ttttcttcat cttttaaatc tcttgaactc attcctattt cagatgttcc
     1261 ttctatggca ttttttatac ctccggaaga accaatctgt tgtacttcta cactaacacc
     1321 ttgatttttt tcttgaaact tttctccgat agtttccata agaggaccta ttgaagttga
     1381 acctgaaaca gaaactttgc ttgaatctcc tgaatcacct gaacctcctg agttagaact
     1441 acatcctact gctaaagctc caactaacat tgtactagct aaaagaacgc ctattttctt
     1501 tttaaacatt ttacttaccc ccgcttatat tttaattttt tgttgtcatt tttctatatt
     1561 ttaataatac agattctttg ttaaatgaat attagagcag ggttaagtaa atgtaaatat
     1621 tgttaaatac atgtaaaaac ttaattttat atgatttatt cttaattaga ttaacttttt
     1681 aaagttactt taggcagata taaacacaat acaactcata ttatcagaag tcttcattga
     1741 agctatcaac ctatcacatc ctataattct ttttctagtt tattaactaa ttcagcaaat
     1801 acatttaagg cttcctctat agattctttt ttagtcatat ctacaccagc agattttaat
     1861 tgttctagtg ggtattctga ccctccactt tttaagaagt ttatatatct atctactgca
     1921 gtacttcctt ctgaaagtat ctgttgactt aaagcacttg cagcagaaaa ccctgtggca
     1981 tacttatata catagaagtt tgaataaaaa tgaggtattc tcgcccattc tattcctatt
     2041 tgtttatcta ctattgcaga ttttccgtaa tacttttcat ttaatttgta gtatatatta
     2101 gtaaattcat cagcagtaag tggttctcca ctctctaccc tttgatgtac aattttttca
     2161 aattcagcaa acatagtttg tctatgaact gtagttctaa attgttctaa ataataattt
     2221 aataagtata tagtttcatc cttagactta gaattttcta ataaataatt tataagaagt
     2281 aattcattta atgtagatgc aacttctgca acgaatatct tatagtccga atataaaaat
     2341 ggttgacttg ttcttgaata atgactgtga acagagtgcc ctaattcatg aattaaagtg
     2401 aatagagaat taaggtcatt tttatagctc attaatatat aaggatgtga atcataactt
     2461 ccccaagaat aagctcctcc ttttttacct tcattatcat atacatcaat ccacccttca
     2521 tcaaaagcac tttttatgac cttcaagtat ttttctccta aaggtttaag tgcctctaat
     2581 atgatatcct gtgctttatc atatggtata tccatgtcaa atttatctgt caaaggaaca
     2641 tacaaatcat acatgtgtat ttcattcaat cctaaaaatt tctttttaag ctcaacatat
     2701 ttatttagat taggaatact tttatctata gctgatataa gattattgta aacatctaaa
     2761 cttacatcat ctgaaaataa agacgcttct attgctgaat tatactttct agtttttgcg
     2821 taaaatatct ctgattttat tccaccataa agcgttgagg caaatgtatt tttatatttc
     2881 tcatatgttg aaaattcacc ttcaaaagcg tcttttctaa ctctactatt cttacttttt
     2941 ataaataaag agtagtttga atgagaaagc ttaaccttat taccctcctc atcttctatc
     3001 tcaggaaatt ccatatctgc atatgctaac atatcgtata cattttctgg aattgtcgat
     3061 aattctgatg tagctgcaag tatttcttcc tctcgttcac tcaaagtatg tggtttatct
     3121 ctcaatatat cctttatata ttttgtataa aaggatagct tctcatcttt taagtattca
     3181 cttaacttat tctcttctat cgccagtatt tctggaacta tataagaagt agccacacct
     3241 aaatcagtag ataacatttc tgtcttagtg gctcttcctt gattatcatt aattctagta
     3301 tcttcatgtt gtttcatatg agtatataca tataaatttt gaacaatcct agatgcattt
     3361 tcagagatat ttaatacttt atataagttc tccttactct cagaaagctt ccctttatat
     3421 tcttttactt cttggataag attttttact ttacttatgt ctttttcaat ttcctcttta
     3481 ctggagtaca tcttatctat cttccatttg tacttttctt ctattgtact tctatcagtt
     3541 cccatatcat acacctctat tttaatttgt tttatatttt attcacttta caaaatatat
     3601 tgtatatatt ttagtccaaa taatctacat ttattactcc ttctatcata tttatttctt
     3661 tcataacgtc gtgtaagtgt attctcttag cataagatac aaaaaaatta accttccagt
     3721 attcttcatt acgacattct atttccatat ttcttacgat aagttgattt ttttcaaatt
     3781 tatcatataa aaaaggtata gagtccaaat tgcttaattt taatatcaat acattctttt
     3841 cttggctaaa taaaaacgta ttgattatcc taagcacaaa taatgctatc attacaaata
     3901 atccacctag aattgccaat tcataataac catatccaac tgctatacca atacatgcag
     3961 ttgcccacat cccagcagct gtagtaagcc cttttactcc acctttattt tttaaaattg
     4021 ctcctgcacc taaaaaacca attccagata atacttgagc aggcattctt gctgggtcag
     4081 catttgtata caaattataa tcaaagaata attgaatcga agttattgaa acaatacaag
     4141 aacctattgc aattagtata tgtgtcctaa atcctgcaaa ctgtcttatt ttttctctct
     4201 ctaagcctat tattcctcct attattagtg ctaatagtaa ccttattgct atctccttac
     4261 ttcctatatc aatcataata taaaaccctc ctgacttaat tacaacttat gtcctacaga
     4321 taatttacac atcagaaata ctattttata tcttaatatt attattaata tatgtaaaat
     4381 atagccatct gttaattttt gatacaaaaa aacccacaac tgtgggctat ttgcttaggt
     4441 acatgtattt atttattaat atatctattt tcttacttga ttctactaac tcttcttgaa
     4501 atatttctgg atattctatg tatgtattta tctcttccct aactgcttca atctcatttt
     4561 ttaacgcttc taattcattg ttcgccataa taatcaccac tattacaaat ttgtatattt
     4621 ttatatacat tttatcaaac aatattatat tttgtcaata ttatgtcaat aaaacttact
     4681 ataaaaatgc tttaatttag gtattgtata cctggatact aagcctacaa acataccagt
     4741 tactaatgat gccattaaca taaatggtaa gtaccctata gcaagtatat tttctatgat
     4801 taaacttgca actattaatt gacctatatt atgccctatt gcacctataa tgcttatccc
     4861 tattagtgat aaatctttta ttttactcgc taaatacata aataaaagac tcagataagc
     4921 tcctcctata ctaaatagta gatatgataa agggcctgca aatattgatg acaaaattac
     4981 cctgatagta acaattataa cagtatcttt gacacctaac aacattaatg aaattaaagt
     5041 tataatattg ctcaaaccca attttgcccc tggaaatata actataagag gatttggtat
     5101 atatgtctct agtacatata aagctaaact ataccctatc ataagaccta aaaatgtcat
     5161 ttttctagtt ttcattaagt taactcctcc aaattttatc tatcatctta cattataata
     5221 caaaatatat aaaaacacct aatttagact aaaatatttt taatctattt agaataaata
     5281 ttagttattt caatgtgcaa ttacctctat aaatcttgac tttttgtatc atacttgtta
     5341 caattttaat gataatgaat ttcattagga ggtgttaata atgactgttt acgatttaaa
     5401 aataggagaa aaaggattta ttaatagtat agatggagat attaaattat ctaaaagatt
     5461 gcttgcactt ggatgtataa atagtactga aatagaagtt aaaaaggttg ccccacttgg
     5521 agacccaatt ataattagat ttagaggctt tgaccttgcg attagaaaat ctgatgctaa
     5581 aaatatttct ttacaataat agaggaggag ctacccgtga ttaatgtagc attattagga
     5641 aacccaaatg tcggtaaaac tactgttttt aatctactta caggttcaaa tcaatatgta
     5701 ggtaactggc ctggtgttac tatagaaaaa aaagaaggat ttcttggaaa agagataaag
     5761 gttgtcgatt tacctggtat atatgcaatg gatactttct caaatgaaga aaaggtttct
     5821 aaatcatatt tagaaaatga agatgttgat gtaatagtaa atgtagttga tgcttctaat
     5881 ttatcaagaa acctttactt aacaactcaa cttatgcaat ttaacaagcc tatagttata
     5941 ttgcttaata tgttagacat tgcagaatct aaaggagtta atatagatgc caaaaaatta
     6001 agtgaagaac ttggagtaat tgttgttcca attatagcaa aaaagaaaaa tggtgttgat
     6061 aggatagaag aagagattaa gggagctacg caaaaagctt ttatatatca gaaaaacttt
     6121 ggaagtgaaa cagaaacata taaaaaatta gaatccattt tatcgagatg tacaaaaact
     6181 acatatacac aaaagaaatc tatcagtgat aagatagaca atatagtctt gaatcctata
     6241 ttagcatatc cgatatttat tggtgtattg ttcctcttat ttaaatttac ctttgactgg
     6301 gtcggaggac cactacaaga gggatttgct agtcttatag aggcttatat ttctgcacct
     6361 gttagtgata tgttatctaa ctctagccca tggtttaaat cacttatagt tgatggaata
     6421 attggaggtg ttggtggtac tttaccattc ttccctttaa tatttacatt attctttggt
     6481 atatcgttgt ttgaagatag tggatacatg tcaagaacag catttttaat ggataaagtt
     6541 atgagaaagg ttggtctatc tggtaaagct tttataccta tagttatggg tatgggatgt
     6601 tcttctccag ctataatggc aactagaaca ttggaaagcg aaaaagatag aagagttaca
     6661 gctcttattg caccacttat gacttgtgga gcaaagctac ctatatatgc actatttgta
     6721 gctatattct ttccacataa tgctgcttta gtaactacgt cactgtattt ggtgggtata
     6781 gttatggcaa ttgtagttgc attattctta aataaaacag cctttaaaac agaggctgaa
     6841 ccatttatat tagaattacc agaatataga atccctacta tagatgcact tattaaaaat
     6901 acttggaata aatcaaaagg tttcttgata agagttgtta ctgtcatgtt tgcaatgtca
     6961 gtcgtaattt ggggactttc ttcattcaat gtttttggat atacagaaga tataaatgta
     7021 agcttcttgg ctactctagg tcatattata tctcctatat tcaaacctct tggttttgat
     7081 gattggagaa cttcagttgc tattttaagt ggtcttggag caaaagaaat cgtagtaaac
     7141 tctttaaata tactatatgg taatctaact gttgtcttac caacaatatt taatggagtt
     7201 actgcatata cattcttgat atttactgct ttatatactc catgtatagc tgcacttgca
     7261 actatgaaaa aagaatatgg caataaaatg atgtttacat catttgcata tcaatttata
     7321 ctagcatgga taatggcttt tatagttaaa agcataggtg gagtactatt tatgggtaat
     7381 tcaataatgg aagctttgat aggtggaata attatagtcc ttgcactctt tattctgttt
     7441 aataaattca agtctacaaa aaacggaaat gtctgtagtg gatgttctat ttgctctgga
     7501 tgtccaagtg ctaattcttg ctcaagtaag gaagacagca ataaaaaaag taatacacac
     7561 taatatataa atattttagt ttaaaataag ataaaagtgg gtgtctcaaa attaactttt
     7621 tagttcatga ggcactcttt tttatataaa atcaaatata ttttcatcat ttcaacagtc
     7681 aaaaagaggc ttatctctat tttgagacaa cctctttttt tgttacttat tcagtttctt
     7741 gctacctaac ttcatgtgga acctagtatc ttttggaaca cttgggtcta cgcctcttaa
     7801 tgtaggtatt tccccacata ttagttgtat aggtactata aaattaaatg tttgatagta
     7861 aggattatct gtgatatcaa tctttaaatt tttatcattt tctgtaacat ctctacctat
     7921 agcaaataca ttttccgtcc aaccagaaag tacatcaatc atcttagtta ctctaggttc
     7981 ttttcctgta tctagtataa atatagtaga atcactattt atagcattat atataccgtg
     8041 tataaactct tcaaactcat acccagttac aggtattctc attgtttcca ataattttaa
     8101 tgctgcttct aatgtatctc catatatatc agagtgacct attattctta tttctttaga
     8161 attaactaac ttttctttgt ttctttcaat ccattgttta gagagcttat acactgcttc
     8221 aaatctatta attgcatcta atatcttatt tatttcctca ttgtactttt cactagatat
     8281 tgtacctttt tctctagcta tttgaagacc taataacatt aagtttaact ttgtacaata
     8341 atatcctttt gttttagctc cagatttttc ttcaccacaa tttactgtta atatataatc
     8401 agaaatctca tctattaaag cattcttaca acctgccata gatgctattt tacaaccctt
     8461 atcttcagct aacttcatag cgttgtatgt agaataacta ctcccacctt gagatacacc
     8521 tactactagg gtattttcat tatcaaactt gaatgtatct tcagttatca taaatggata
     8581 cattttcaca acatctatat ctaataaatt ttgtaaatat ggttgcactt gcactcctga
     8641 gtgatagcta gtacctgaac ctgttattat aatctttttt agatttgctc tcttaacttc
     8701 attaaataag ctatctgcat tagatataat ttctctcatt ctaactggtg tttctaacat
     8761 ataatcttgt attgtcattt tatacacctt ccttatctat atattcaata aattataatt
     8821 aatttctata acaaacctat agcatgtaaa gcgattcctg ttactatagt gcctatagtt
     8881 aattttgtta cacttacctt tctcttcaac atgtaatata ttaaaaatgc aaaggttaaa
     8941 ggtaacatat taggtattat tttatctagt atatcttgta aaattacttc tgcaccattt
     9001 aagttaaatt tcaaaggagt agttatactc aacatagttg caaccattcc tcctacaacg
     9061 aataaaccga cagttgtagc catagacatt atcttctcca taagtccctc tctttgtatt
     9121 ctttcaagag aatttacccc tactttataa ccatacttaa gtccaaatat tctaagggca
     9181 aatgctggta tattgtataa taataagaat aatagtggtc caaagatatt gccttctttt
     9241 gcaagtgata ctcctactcc tgctgctata atcctaaatg ttccccagaa taatgaatca
     9301 ccaattcctg caagaggtcc cataagagct gttttaacag cactaattga actttcatca
     9361 aattctggat tattagcatt ttcttcttcc atagctgctg ttatacctaa tatagcagga
     9421 actaccactg tcgttgtatt aaaaatctct aaatgccttt ctattgcttt tgcttgattt
     9481 tctttcttat tatatagctt ctttattgca ggtaacattg aataacaata acctaagttt
     9541 tgcattcttt cgtaattaaa tgcaccttga agagcaaatg aacgccaaaa catacttctt
     9601 aattcttttt ttgttaattt attcaaagtc aatctctcct tccgttaaag tattcattgc
     9661 taattgagga ttttgattat tttctttgtt attgatataa aagtttagta caaatgctac
     9721 acatactcca aataaagcta tagcagttat atctaatcca ccataagatg ctaacaaaaa
     9781 ccctataaag aaaaatacag acatcttttt tgaaatcaac atatccataa gcaacgcaaa
     9841 acctactgct ggcaacaatg ttcctgcaac acccaatccg tctaatataa ctttaggtat
     9901 tgcatttatc aatgttgtaa ctttatcagc accaatcata attgctaaaa aggttggaac
     9961 tgatgtactt aaaaagaata atattactgg caaccacatc attatggtaa ctttcttaaa
    10021 atcagcctgt ttagcataga aaactgcctt ttgagaaaag tacgaattta ttattctaac
    10081 taatactcca agagattgtg ctaatactgc tactggcact gccactgcta aagcaacttc
    10141 tgcaccatta cctgataata tagcaaaagc tgtacccaat acacctcctg ttactacgtc
    10201 tggaggagta gctgcaccta tccctgcaat tcccatccaa attaattcaa gctgtgcacc
    10261 aatcataatt cctgatttta tgtctcctaa aactaatcca actaacatac ctgtaactat
    10321 tggcctatca aacatagatt gaccaaatat acgcccatct agtataccaa ctcctgctat
    10381 aatagctaac atagtagctt gtactaacat attgaacctc cattttaact tagtttaatt
    10441 tacattaaat tacattacat ttttgatttt atttcgattt tctaataact catttacata
    10501 aacaattcca ttttttgctt cctctaagcc tgatttaact attagttttg tatcctcctc
    10561 aactaataag aatatagata aaagcattgg taaatttacg cctgtcaaca attcaaattt
    10621 gaacccttgc aaaagtaagt ttgtacatat atttgccaca cttccaccaa acacatctgt
    10681 taaaacaaca aattcttcac tatctttatt atttgataat atacttctaa tttctttctc
    10741 agcatcatct gctgatttat cttttgtcat acaaaaatac tcaatattag ctaatgaacc
    10801 aattattaat ttacttgtgt caacaagttc tctagacaat tctccatgtg ttgcaattag
    10861 aaattttttc attctctttc tcctaaatta ttttattaaa tctgatagtt gtttcttttt
    10921 gtctctaggt aacattctaa gttctatatc aattcctaaa tcacctattt ctttaaagtt
    10981 atttatatca tcatcatcaa cagcaactaa atctgtaagt ttcttttttc cttcttcata
    11041 tcttaaccct cctacattta tttcttttaa tactcctcct gaatttttag ctacaactaa
    11101 agcatctctg gtatttttta ctaacactaa tacattgctt ttctttgaag aagcaaattt
    11161 ttctactatt tctccagatt cctctacccc ccttatatat aaagttacat tgctaggttt
    11221 cgctaaattc agtgacatag ctttcatttg gtcatttttt gcttcatcat ttgcaactag
    11281 tattacattt atacctagtg atgttgccca tccatatgca acctgaccat gtatcaatct
    11341 gtcatcaatt ctcatcaaag ataccatttt ccccaaatcc tccatcatac attaaaattt
    11401 ttattgttta tactctcatt tatctataaa agcaaaaagt atgccaaata ctggcatact
    11461 attttaaatt tcatttactt attttgtaat cttgtatttt actgtggtag accatagata
    11521 atattataaa tatatcctat ctctgaaatt ggtatttgca cactataact cttttctagc
    11581 acactaaatg cattttttat tagttttact tcactatgat ggtttttgat taacaaatcc
    11641 atatcagtat actcttcaat ttcagtttgt ctaactaatc tttcaatcat acaacttaaa
    11701 tgaacatata aagaaatctt tttatcattt ggaagttttt tcttttgaat attttctata
    11761 gcattgattc cttcttctat tctctctatt atcttttttg aatctaaaat agttagactg
    11821 tctattactc taattaatga aaaattcttt acaatagaat catttatgtt ctttattttt
    11881 tcattttcta ctactctact aaatattcta aaaacatcat cttctccttt tcctgatatt
    11941 atatcctcta acaatataaa atccacccca ttcacatttg gattagcagt tcctactata
    12001 gccatcacat catacatttc aaaaactggt tctttatctc ccatagaaac aagactatca
    12061 taatcataag gtattatttt gacatcaact atcccaatta aactttcgga aagtagatgt
    12121 tggattttta tagcagtacc tatccctgta aaacaagatg atattatagc atattcttta
    12181 tttttttgag gataatacaa ttgtatatta ggtttagttt cttcatccat aatttctgca
    12241 atctgttcaa tgtttttacc ttgcattaat aaatttccta cttctagtgc agaaatagtg
    12301 gatatttggt ctataaataa cattggccca tttatcttat tttttaaatc ttcatagata
    12361 agattaagag agcccatatc aaataacaca attaaaccat tctttatcct atctccttct
    12421 aggtatctag acatttgttt tgatacatca gcaatggtag catctatagg catatcaata
    12481 gctgtaaata tatttttacc caacatccga ttacatacat tcgccatact actggcagta
    12541 gcatatccat gtgctataat agtcgcatta acttgattaa atttcattct tataattgtt
    12601 gatttaataa atataatcat tatgattgta tctacattat taagatacat atccaatcta
    12661 ttcgttatta atgttgatat tttacttatt atagagtatt ctttgtaact ctcttttttt
    12721 aaatatatta gtaatttact ttctaaagct ttatactcat cagataattg ataagattcg
    12781 tccttcaaat aaagataaga cgaaaatgcc aatacattat tcccatcgta ttttatattg
    12841 ttattaactt ctaaatttct aaatatatcc tgcattatcg atattataag ctccataagt
    12901 acgttattat tttcatcatt atttgaaaag actaaattgt ccattagtct aatcattaga
    12961 ttagaactct ttttactaaa tatttctttt gttatttcat ttttttctag tctatcaaag
    13021 tagtctaaac attttatgaa actttgatag attatatttc ttttatttct tccatttgac
    13081 atttcaatac taaattgttt aggagaaaag ataatgcttt tgcttttacc actttggaat
    13141 tgaaatatat ctacattttc tatataaaga tttctaggga ggtcttgaag agtaacaaaa
    13201 atatttccat ttcttctttg attttttgca taagctcgtc cacatgcata ttttattaca
    13261 tttttacatt cacctatatt ccccttgaat tttatcttta aaatcgtatt taatgtaatt
    13321 tgagttatct ctataggcaa gccaagtacc ttactttctt cataaataaa atgttctaca
    13381 atctttcttt tttcaatagt ccctctttct tctatattgg gaacataaat atttatgggt
    13441 attcttctta aaaaagtttg caaaaatttg gatagacttt ctgttgttgc aaatattaat
    13501 cttaccctag cttttctaat tactccattc tctccaatcc tagaaaacgt acctgtatcc
    13561 ataaatgtaa ataacttttc ctgaccttca ctatttaatc tatgtacttc atctaaaaat
    13621 aaaacaccac catctgattg ttctattaga cctgctctag attcatttgc tccagtatat
    13681 gctcctttta catgcccaaa taatagacta gacaataact ccaaattatt ataatactgc
    13741 gcacagttta acacattgta tggagaattt ttttctaata cattactgct tacactatat
    13801 ttgtatatta tcttggctaa aaaagttttt cctactccac tttcacctgt cagcattatt
    13861 gtaagtccgc catttggata aagtacagcg gtttttattt gttcgattac ttcttttaag
    13921 ctattatcat atcctatgat actattaaac acagtctctt gctttaataa tttacactca
    13981 tttttaagtt catctaaatt ttcataaata cttttttttg taggtcctac tacatcattt
    14041 attttatttt taggtaaaaa aataacaggt ctactgttta tctttataag attatcagat
    14101 tttactaaat catttaataa tctacttgat tgagttctac ttaaattaaa cttttttact
    14161 atatcttctg ttattatgcc actttctttt ttaagttcat caaagttgtt aaataataca
    14221 cattcattta caaaacttaa aatttcatcc ctcat
//
LOCUS       DAELSY010000102        14182 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.102, whole genome shotgun sequence.
ACCESSION   DAELSY010000102 DAELSY010000000
VERSION     DAELSY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14182
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.102"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(250..888)
                     /locus_tag="KPG09_002651"
     CDS             complement(250..888)
                     /locus_tag="KPG09_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG0967248.1"
                     /translation="MKGLILKDLLNLKGNVKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
     gene            complement(888..1751)
                     /locus_tag="KPG09_002652"
     CDS             complement(888..1751)
                     /locus_tag="KPG09_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967249.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRNPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            complement(1748..2122)
                     /locus_tag="KPG09_002653"
     CDS             complement(1748..2122)
                     /locus_tag="KPG09_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0967250.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            2489..2893
                     /locus_tag="KPG09_002654"
     CDS             2489..2893
                     /locus_tag="KPG09_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBG0967251.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNICFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            2910..3506
                     /locus_tag="KPG09_002655"
     CDS             2910..3506
                     /locus_tag="KPG09_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967252.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            3543..6137
                     /gene="clpB"
                     /locus_tag="KPG09_002656"
     CDS             3543..6137
                     /gene="clpB"
                     /locus_tag="KPG09_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBG0967253.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSDDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(6334..7761)
                     /locus_tag="KPG09_002657"
     CDS             complement(6334..7761)
                     /locus_tag="KPG09_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0967254.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            7903..8799
                     /locus_tag="KPG09_002658"
     CDS             7903..8799
                     /locus_tag="KPG09_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0967255.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDFLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            9464..9604
                     /locus_tag="KPG09_002659"
     CDS             9464..9604
                     /locus_tag="KPG09_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967256.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(9601..9972)
                     /locus_tag="KPG09_002660"
     CDS             complement(9601..9972)
                     /locus_tag="KPG09_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0967257.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            10521..10778
                     /locus_tag="KPG09_002661"
     CDS             10521..10778
                     /locus_tag="KPG09_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967258.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(10888..11568)
                     /locus_tag="KPG09_002662"
     CDS             complement(10888..11568)
                     /locus_tag="KPG09_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG0967259.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
     gene            11828..12127
                     /locus_tag="KPG09_002663"
     CDS             11828..12127
                     /locus_tag="KPG09_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967260.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            12451..14106
                     /gene="ilvD"
                     /locus_tag="KPG09_002664"
     CDS             12451..14106
                     /gene="ilvD"
                     /locus_tag="KPG09_002664"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBG0967261.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIEFNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
BASE COUNT         5710 a         1593 c         2093 g         4786 t
ORIGIN      
        1 tttaatacat aaaaactatt ttagatgtta ttctattgct atgttcaata atatcattag
       61 tagttcaatt tataaaatga ataaattata taaataagat tagaaagtag taaatagtct
      121 tattaaatat aagtaataaa acaataaata cctagtagat attaataata aaaaaataaa
      181 atactaccaa aataaacttg atagtatttt atttaaaata cttaaataca gagacatgta
      241 tagtatattt tatacttctt ttttactata gatttttgat gatataaaat atgaaatcac
      301 aaatagtaaa atagcaacaa atggaagaga atatactagt gtattaagta taatttctat
      361 agaaataggt gaactaatat tttctaaaat acttttaaaa actaataaag ctagtgttgg
      421 aactaaaaag cataaaatca ttaataaacg agctctttct gtaccaaatt tataaataag
      481 tggtagaata aggctaccaa aacatactga aattgataag attagtgcat taataagtag
      541 tgtttcattt aatataagtg tatttttaaa ataaccaata gttacactaa caataagtga
      601 aacaagaaca cctataaagc taaatattaa catagttaaa tatttactta atacaatatc
      661 attgcgatta ataggcattg taagtacata actatcccat ttatttaaat catcatatga
      721 aaatgttgaa acaatcattg tagtacataa aactataata acacctataa agttattaac
      781 atttccatct cctaaagaag acataaaccc aaacataatg atgaataaaa gtataaactt
      841 tacatttcct ttcaaattaa gtaaatcttt taaaattaaa cctttcatta tttatcacct
      901 ctcacataaa ataacataat ttcttctata gacgtattat caacaataca atttggattt
      961 cttgatataa atctatgttt atctttaaga agtacttcat atccaaattg gcttttacga
     1021 taatatgaat aatcctctct tgaaaggtta tcaaaatcac taggtttaca ttttaaaact
     1081 cccatagtat caattaattc atccttattt tcactaaata caatttttcc tttatgtata
     1141 aatgtaatat agtctgctat cttatctaaa tcacttgtaa tatgtgaaga taagataata
     1201 gagtgttctt catcttgtat aaaatctaaa aatatatcta gtatttcttc acgaacgata
     1261 gggtcaagtg aactagttgc ctcatctagt attaataatt ttggtttatg agataaggct
     1321 acagcaatac taagtttcat cttattcccc tttgaaaact ccttaattaa tttattgtct
     1381 ggtactctca atttttttaa gtagtcttta aataatgtgt cattccaatt tttatagatt
     1441 ttactcatga tttttgaaac atggtcagtg tttaaatttt catgaaagtt gcttccctca
     1501 aatacaaccc caatttgctc tttaatttct ttttcatttt ttttactatc aagaccaaat
     1561 atttctatat ttccactatc ataagatatt aaatttaaaa ttgcttttaa tgtagttgtt
     1621 tttcctgcac cattttcacc tacaaatccc attacactcc cttttggtac tgaaaaagat
     1681 atatcttgta ggctaaaatc tttataagtt ttatttaaat tttttatagt tattgcatcc
     1741 atacttatca ttctccttta tataataaaa ataaaatatc ttttaattcg tcatatgtaa
     1801 tatcactact ttttgctgta tcaactgctt tttgtaggta ttcttcaatt aaacgaaatt
     1861 gttcttcttt gacaaaatcc atgtttttac tagatacaaa actgccttta ccagtaactg
     1921 tctcaataaa tccgtctcgt tctaaatcgc tatatgctcg ttttgtagta attacactta
     1981 ttctaagttc ctttgccaat agtctcatac ttggaagagc tgagccttct ggtaactctc
     2041 cgcttataat catactttta atttgtgtag taatctgttc atatattggc ttcccactcg
     2101 aattactaat aactatattc aataagtcac ctccttaaat tgtatatata gtatatacac
     2161 attatataaa tatatatata atatgtcaac tgattttaga aaaaactaat taccaatatt
     2221 atacatttaa taaatgtaat gtattttatg aaaggctgcc atattaaata gatttaaatt
     2281 tttttaaaaa atttataaat attattcaaa aatcctgcca ataaattgga aatactaaat
     2341 taaaaaaata ataaagttta gcttatagct aaacaaaagt ttacttataa ggtaaacttt
     2401 ttgcattttc agttttgaca tacatatttt tttgtaatat atttatatta tcaattagag
     2461 ataaggagag tgagaagaga tatttaattt ggataagaca ttaaaaaagg atttaaatca
     2521 ggaatataca gagagcaaaa gtttagttat gaataaaagt aagtatatga atatttgttt
     2581 taagaattat attaaagtag tagaaggaat gtatactaag agggatttaa ttccattaaa
     2641 attaataaac tctatggata aaaataataa gattagaaaa acattatatg aattatcttg
     2701 tgaatttgaa tatccaaaaa cttctttatc ttcattgttt acggagttga aaaaaatgga
     2761 ttttttacaa agagtgaaaa atggtgagta tatgataaat ccacatatag catataaagg
     2821 tagtagagct gataaagaaa gtttattagc agaatataat cagataaaaa gacctaataa
     2881 ggttagtaaa taaggaagaa ggtttttata tgagtttaaa acttgataga aatgttctac
     2941 agtggtttga ctatgtattt gagaatgaaa aaactagttt gagacactat aattttaatt
     3001 gtacactaaa ggagattagt tctaccagtt tgaacaaagt agcttttatt ttagaaaaaa
     3061 ataactctaa gtactggaag ctttattttg aaataccagc tgaagttact ttaaaactta
     3121 agcaaaacat tcatccttta tttagggaat atatatacga acaaatatca ctgtataaca
     3181 ataatcaaat ttacaatttt gtaaattcaa acatattaaa agtgtttaat aatattgcta
     3241 tttatcaata taatatttta gaaaatctat acactattga ttttaaaaag agttttatag
     3301 ataaatgtca gtatttatta ataggtgaaa aaagacttat agatgaagac ttgtatttaa
     3361 tagcaaaatc aaaagaagta ttcgattttt ttaattcaga tggaacattt aatttaacat
     3421 tgagtttcga tatacaaaaa aacgagaatt tacttgatag tcttttagag cttagaaaat
     3481 caataattat aaatgagaga atttaattta tttggatata atattaaaat ggagggattt
     3541 atatggacgt agaaaaaatg actttaagag ttcaaaaaag tttaaatgag gcttataacg
     3601 aagcagtaaa aaatcataac caacaagtag atgtaattca tcttttctca gcacttatta
     3661 atcaagagga tggattgata ccaaatataa tcgaaaaaat gaatatatca attgattctt
     3721 tgagaaatac tgtaaacttt gaaattgata agcttccaaa agtatatgga gaaggtgctg
     3781 actcacaagg agtatcagct actagaaaaa taaatgaggt gcttataaag gcagagagta
     3841 tttctaaaga atttaaagat tcatatataa gtgtagagca tgttatgctt gcaatgatgg
     3901 aaacagaatc aaaatcagct gttggtaaaa tattaaaaca atataatata aataagaatg
     3961 actttttaaa tattttatca caagtgagag gtagtcaaag agtagaaaca caagacccag
     4021 aaggcacata tgatgcactt gctagatatg gaacaaactt agtagattta gccaagaaaa
     4081 ataaattaga ccctgtaatt gggagagatg aagaaataag aaggattata agaattttat
     4141 caagaagaac aaaaaataat cctgtattga ttggtgagcc aggtgtaggt aaaactgcaa
     4201 ttgtagaagg gttagcagag agaatagtaa gaggagatgt cccagaaggt ttaaaagata
     4261 agattgtatt ctcacttgat atgggagctt taattgctgg tgcaaaatat agaggtgaat
     4321 ttgaagaaag attaaaggca gttttaaaag aagttcaaag ttctgatgga aagattatac
     4381 tgtttataga tgagattcat actatcgttg gagctggtaa aacagaaggt tcaatggatg
     4441 caggtaatct tattaaacct atgcttgcaa gaggtgaatt gaactgtatt ggtgcaacta
     4501 cttttgatga atatagaaag tatatagaaa aagataaggc tcttgaaaga cgttttcagc
     4561 ctgtaattgc agaagaacct actgttgaag acacaatatc tatacttaga ggtttaaaag
     4621 aaagatttga gatacatcat ggagtaagaa tacatgataa tgctattgta gcagcagcaa
     4681 aactttctca tagatatata caagatagat ttttaccaga taaagcaatt gatttaattg
     4741 atgaagctgg agctatgatt cgtagtgaaa tagattcatt gccaacagaa ttagatgttg
     4801 ttagaagaaa gttatttaca ttagaaactg aaagagaagc attacttaaa gaaaatgatg
     4861 ataaaagcaa atcaagattg gatgatattc aaaaagaaat agcagagtta aaatctaaaa
     4921 atgatgaaat gacagcaaaa tatgaaaaag aaaagagtca aatattagat attaaaaact
     4981 taaaggctca attagacgaa gctaaaggta aagctgaaaa gtatgaaaga gaatatgatt
     5041 tcaacaaagc agcagaggtt aaatatggtg aaatacctaa gttagaagaa caaataaagc
     5101 agtacgaaga aaatatgaat gatggttctg aaaattctct tttaaaagaa gaagttactg
     5161 aagaagaaat atctagcatt gtatcaaagt ggacaggtat accagtaact aaactagttg
     5221 aaggagaaag agaaaaatta cttaaactag aagatgaact tcataaaaga gttattggac
     5281 aagatgaagc agttacagca gtttcaaatg cagtaatacg tgcaagagca ggattaaaag
     5341 atgaaagaaa accaattggt tcatttattt tcttaggccc tacaggtgtt ggtaaaactg
     5401 aacttgcaaa aactcttgct agaaatttat tcgatagtga cgataatata ataagaatag
     5461 atatgtctga atacatggaa aaacatgctg tatcaagact agttggacct cctccagggt
     5521 acgtgggata tgaagagggt ggtcaactta cagaagcagt tagacgtgct ccttattctg
     5581 taatactatt tgatgagata gaaaaagcac atgaggatgt atttaacatg ttcttacaga
     5641 tattagatga tggtagactt actgataata aaggtaagac agtagacttt aaaaatactc
     5701 ttataataat gacttctaat ataggaagta attatctatt agaagcagga ggaaatataa
     5761 cagagactac taacaattta gtaatgaatg aaatgaagca tagatttaaa cctgagtttt
     5821 taaacagagt agatgatatt ataatgttta agcctttaga ccaagaaaat atcaagaaaa
     5881 ttatagatat atttatgaaa gatttaaaaa atagattaaa agaaaaagat attactattg
     5941 aagttacaaa ttcagcaaaa gatgttatgg tgagagaagg atatgaccct gtatatggag
     6001 caagacctct taagagatat ataggaaata cattagaaac tataattgca aagaaactta
     6061 ttgctggaga tgtgtataat ggatgtacta tagttataga tggaaaagat gaaaatattg
     6121 aagttttagt taaataataa aagaatcagt tgagcataat atactaaata ttttgctcag
     6181 ctgattttta tttataaact ataggaaaag aaatatttta tcaaattaat aaaacatttt
     6241 taagtcaata tgtccaaatt aaatttaact caaaggaagt attttagtaa gtttcttata
     6301 atataatcat aatttgaata taattataaa aaactactgt tcatttttat tatatttaaa
     6361 taaataaaag ccaattaata ttgcacctat taagcaaaca agtgtaacaa taatataaac
     6421 atttctcatt ccaaatacaa atattttatc attattaatc acataatcat tgaccctata
     6481 tccagcttta atactcataa aattatataa aagtgttgta gaaatagtaa tacctataac
     6541 ttgtcctaaa tttcttacaa gggaatttat gcttcctaca actcctagct tacttctaga
     6601 acagcttgac attatcaaag cattgttagc aggttgaaat atagcttgtc ctattgatat
     6661 tatcgatata taaattacaa ataaaataag ggcagaactt tctttcaatc ttgacattag
     6721 aaagaatcca aaactcataa cgagaagacc aattaaaata attttttctg acttgatttt
     6781 atctgatata ttcccaaata taggtgaaaa aatggctaaa atcaaaggtg atagtatcat
     6841 aaataaacct gcttgaatag ggggaagttt catagtactt tgtaagtaaa atggaattaa
     6901 aatacttgat gatgcaatac aaatgaatga tgtaagagca caaattaaat tcaatgaaaa
     6961 tttaaaattt ctaaaaattt caagatcaag aagcggttga ggatgttttt tttctaatat
     7021 caaaaataat ataataaaaa taattgataa taatagtgca attaatatat atggatttag
     7081 taatcctgtt tgttgagcac taattagagc actaaaaaat aatgttgttc ctaaaaattg
     7141 aagtatagca ccagttttat ccatattttc tgaagatttt ttactattag gaagaaattt
     7201 tgtatttaaa aagattacaa taagaccgat tggaatattt ataaaaaata taacattcca
     7261 atcaaatatt gacaaaataa atcctcccac agatggtcca accatatttc caatagcaac
     7321 tgcagtaact agaatcccta gggcttttcc tctgctttct tttggaaata gttctgtgat
     7381 aattccatga ttgtttgcca tgtaggcaga agcgcctatt ccttgaataa agcggcaaac
     7441 tataagtaaa gttaaagaat tacttaatcc acatagtaat gaagctgatg taaagagaat
     7501 agtacctact tggaatactc ttgatttacc aataatatct ccaagtcttc caaaaaataa
     7561 tagtgttgaa caaataataa ttgagtaact tgctattacc cattcaatag atgctagaga
     7621 tacatttagt ttgcttgcaa gaactgggag agcaacattt ataatactcg aatctaatgt
     7681 tgccataaaa gtcatggcaa tgactacaaa taaaactgtg attttttttc tattatcatc
     7741 ttttttaaaa gagtttgtca tttttattac ctccttatta taatgactgg cattatgtta
     7801 atgtttacaa tgatagttac attttatata atagcgatat gaaataatga atacaataac
     7861 tatattgctt ataattagca ctattttgat gaggggggaa taatgaagga taaaaataca
     7921 tattttaata ggaaaggtca agaaaacttt atagatattt ttttaaagga tgacattaaa
     7981 aaaggagcaa actttaaaag acattggcat gagcatctac aaatatatta ttttattgat
     8041 ggtatagcat atcttgaatg tggattggat aattttaagg tagtgcctgg aaatattgta
     8101 atagcaaaca gtaatgaatt acattattta gaaagcttat cagataattt aaaattctat
     8161 agtattcgta ttgagcccac attccttttt agtaatcaag ttgatttgtt acaaactaag
     8221 tacttagcac cattggcttt aaatcatatt gtatttaata atttaattca aaatgatttt
     8281 ctgattttaa aatgtattac aactatactt gatgaatatc acaataaaga agttggatat
     8341 gagttatcta taaaagctaa tatctatcaa ttaatagtac tcttattaag aagtcatatt
     8401 gataaaatat attctgaaga tgaatttata aaaagaaagt attctttaga aaaatttgaa
     8461 tgtatctttg acttgataga agaaaaatat atggataaaa ttacattaaa agaacttgct
     8521 agtcgtgtga acttgagtgc acatcacttt tgtagaacat ttaagcaaat aacaggtaag
     8581 actacaatcg attttataaa tggaatcagg cttgataaag ctatatatta tttaaaggaa
     8641 acagatttaa atatgacaga aattgctatg agatgtggct ttgatagcat aaattatttt
     8701 agtcgcttat ttaaaaggaa ttataatata tctccaacta agtatagaaa tgaacataga
     8761 gaagttagta atgttatttt aaaagaagat atgaaataat tcataaatat actttataga
     8821 aaatatatat ttttaaatct ttgagttttt cttaataaat attaataaag tatagtacat
     8881 aaatgagtaa gactacttgt atagttaaat gtgaaaatga gtatgaaaga atgtagtgtc
     8941 tatgaaaagt atattttaat atgaaactaa aattggaaaa ataagaatag cagaaaaaat
     9001 gattatatta taaatcagtt tttttgagac atctggctta gattctgaaa gatatttaat
     9061 caaagaaata aaactatcaa ataagcaagt aaacagctaa aatagtatga tttttataga
     9121 aaatttaaat ccatcctaaa gaattaaaag aaagtcaaat aaatataaaa aaattatata
     9181 tttataaaaa attttataat aaaaatcatc ctgaaaaata tataaaaata gaaaaaacat
     9241 acaagattct acaataatag atttttttgt tattattacg agttttttat tgcaactgta
     9301 gaataaaagt attattatat aattaagata attatctagg gaaattttgg ataatcaaaa
     9361 tatattttat aaaatcaaaa tacaattaca taaactttat taaaaatttg ttgggaattt
     9421 ctgactagca ttaagaacgt aagtttgttg ggggatggta tatgtggaaa agtcaaacac
     9481 tactgttaca aaaaaagaaa aaattgaaga tataggaatg aaattagaac atttattgag
     9541 tgaagatatt actttttatg aattattact tgagattttg tataaagaaa aagaagagaa
     9601 ttaattctct tctttaagga atctttttaa ataggcatta tcaatcattt tttctatagc
     9661 ttctctatct tctttttcaa gagaatatat tttttctaag agcttttttg ttttttcact
     9721 tactgataag ttctctatga ttaatttatc ttctttgctt atttttgtat tatcatttac
     9781 gcctaataaa taatctgttg atgtttctaa tgcttttgct aatctaacta ttatttcact
     9841 tttaggttct cttaaattat tttcataacg tgacaagcta gcctcagtta taccgacttt
     9901 agtagcaagt tctttttgat ttatatctaa ttcttttcgt agatttgcta ttctatctcc
     9961 taaactttcc atatgtacat cactccttac cgaaattata agttattttg ataaaaagta
    10021 aaataaaatt accaaaaata taaaaaaaaa gggttaaaat aataaaatat attgaaaatt
    10081 accatttgta tatgtataat atgaattata caaatggtaa tttgatgaga ggtggtatgt
    10141 tgaaataaga aactgaatta attaataaat atatagaaag ttttgaaaac ccttaattga
    10201 gatataaaca atatgtacaa aattttttgc aagtaaaatt accaaatgta taaaataaat
    10261 cagtagcata gataatttta tgctttaata cattagatta tttaatttag ttataatggt
    10321 caaaaatgca atctacaaat aaaacaaagt attatcaaaa aagtattctt taagagaatt
    10381 ttttattaga gatgtaattt atataaataa tcgtattaaa aaataaaata ctaagtttaa
    10441 aaatttaaat aatctataaa tcaattataa aatattattt cttagaaatt aaaatgaatt
    10501 aagtaaatgg aggaattgat atgataataa ttagaagtca agataaaact aatttaatgc
    10561 agataaacca aattaaaata gatggtagtc aagtttatgc agtatttgaa agtaaaattg
    10621 atactgtaaa aataggagat tatgaaaata atacaagagc tatacaagtg ttggacaata
    10681 tacaaaactt cattgaaaat ggaacaaaat atgattatat aacatcaaat aaagtcagat
    10741 acaatgttaa taaaatattt cagatgccag ctaaatagtg agtgtgcatt ttagtttaat
    10801 aactaaagcc cactttcttt tgtagtatga aagagggctt tacatatttg taaatatcga
    10861 agtacacttg ttttatcaat aaattattta ataaggtata atggaccaat taaacaataa
    10921 aaattctcct aaaacagcaa gtattaaata tataatagct tcatttttgt aaagagaggt
    10981 aatttttttg cttatgtcat catcaattgt aatagcaatt tcatcttgaa taatattttt
    11041 atatatagat gggaaaatat cttttttctt tataagatta aataaattat ttacacctac
    11101 ccatataaaa tatcctaaaa cagcacctaa tgtgttcatc attaaatcat caatatctgt
    11161 agctcttaag ttaaacaatt gacaaagttc aattgctaat gaaaaaccta atccagttaa
    11221 tgaaacattg ataatatttc taaaattttt ccaaattaaa ggtagtaaaa atcctaaagg
    11281 cataaacata attatattta aaatatatgt taagatgcca actgagtcaa aaggtattag
    11341 atttatttca tcaattctaa aaagcccact ataccttcct atatcccata tgctaccaat
    11401 tccagcttta ctaagtgcta gatagatata taataaaaaa atatataccc atactaaatg
    11461 tatcattaca ttcttttcaa ctttcaaatt ttttcgtttt ataacgacta tttggtatat
    11521 taagcatggt aatataacgc aaataaatga ataaaatgtc catatcattt tattttgtcc
    11581 cctttcaaat ttatgttttt agttttataa gtactatctt tatattaaac tgtaagtttt
    11641 aaaaatcttt taataaaatc ttaaaaattt attaatttat aaaaatatat tattaagatt
    11701 aaaaaatata tataatcaaa gtttctacaa ttatatagtt aggtatataa aaattttatc
    11761 gtatgtataa atattataaa tatatggaaa taataatagt atattttggt aaagtaggag
    11821 gaattttatg aaatggatta aatggtatag tattacatgc atatgtattt ttatagtagt
    11881 agctttttat atgtttatat ttcctaataa aatagaaact attgatactt catctgcata
    11941 tagctttgtt gaaaagaaag taccaaatag tgctgtatat caaggataca aaaagaatcc
    12001 agttgatggt actactacaa tttattacag ctatgataat tcaactcata tagttagatt
    12061 atcacatcca gaagactatt caaggaagat taattgggat aaagtttcaa acatcagatt
    12121 tgattaatct ttaatattta taaaagtcta tatatctaag gcaaatatat tattaatctt
    12181 attcatagtt gagcactttc tattatagaa gtgcttcttc tgatttatat cataaaatat
    12241 tactcaacaa tttgtaaaat atattagttt ataaaatata ttgaaaagat aatattatat
    12301 aatacgattg tgtaagatat tgataaaaaa tcgtaataaa ttttcattag tatatgctaa
    12361 tattttttta caattttcgt aattaattaa tagaaaaata tattataata atattatgaa
    12421 aatttaaaaa agtaagaaag aggtaatatt atgagaagtg atataaaaaa aggaatagaa
    12481 ggagctccaa agagagcgtt aatgtatgga atgggtctta ctaaagaaga aattgaaaga
    12541 ccattaatag gtatagtaaa tgcacaaaat gaggtgattc ctggtcatct gcatcttgat
    12601 gaaatagcag aagctgcaaa aaatggagta agaatgagtg gtggtttgcc attagaattt
    12661 cctgctatag gggtatgtga tggtatagct atggggcatg tcggaatgaa ttattccctg
    12721 gcttcaagag aattaatagc agattcaata gaagctatgg ctatggcaca tggttttgat
    12781 gctttggtac ttatacctaa ttgtgacaag atagtgccag gtatgttaat ggctgctgca
    12841 agacttaata taccaagtat tgttgtgagt ggagggccta tgcttccagg taaaaaaaat
    12901 ggaaaagtat atgattttaa ctctgcaatg gagggtgttg gagcatgtaa agatggaaca
    12961 gtaagtgaag aagaattaga agagttagcc atgaactctt gtcctggatg tggttcatgc
    13021 tctggacttt tcacagcaaa tagcatgaat tgtttaacag aagctttagg catgggtata
    13081 ccatataatg gtactgctgc gtcacattct ggagaaagaa aaagaatagc aaaatatgca
    13141 ggtatgtatg ttatggagtt acttaagaac gacataaaac ctagagatat tttaacaata
    13201 gatgctttta aaaatgctat agctgttgat atggcaatgg ctggttctac aaatacagta
    13261 cttcacttac ctgcaatagc ttatgaatca ggaatagagt ttaacttaga tttttttgat
    13321 gaaataagtg aaaaaactcc ttgtttaaca aaattaagtc caagtggaaa acatcatatt
    13381 gaagatttac atatggcagg aggaatacca gctataatga acgaactttc aaagataaat
    13441 ggaataaatt tagattgcaa aaccgtaaca ggcaagacta taggggaaaa tataagaaat
    13501 tgtgaaatag aaaatgaaga agtaatacat acattaaaga atccatatag taaccaaggt
    13561 gggcttgcaa tattgaaagg aaatcttgct ctaaatggag ctgttgtaaa aaagtcagca
    13621 gttgcagaag aaatgttagt tcatgaagga ccagcaagag tttttaattc agaagaagaa
    13681 gctgtaaatg ctatttttgg taaaaaaata aacaaaggtg atgttatagt tataagatat
    13741 gaaggtccaa agggtggtcc aggaatgaaa gaaatgctat ctcctacatc agcagttgca
    13801 ggaatgggac ttgataagca tgtagcactt cttactgatg gtcgtttttc aggagcaact
    13861 agaggagcat ctataggcca tatttctcca gaagctatgg aaggtggttt aatcggacta
    13921 gttgaagaag gagatataat ttctataaac ataccagaca aaaaattaga gttaaaagta
    13981 gatgaagttg aaatagaaaa tagaaaatta aaatttaaac ctttagaacc gaaaataaag
    14041 catggatact taagtagata tgctaaattg gtaacatcag caaatacagg agcagtttta
    14101 aaatagtgaa ctatatttac gaaaattaga aaaaattaag aaaatattaa ttggatatct
    14161 taggtgttat agcactaatg ag
//
LOCUS       DAELSY010000103        13989 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.103, whole genome shotgun sequence.
ACCESSION   DAELSY010000103 DAELSY010000000
VERSION     DAELSY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13989
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.103"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            159..1286
                     /locus_tag="KPG09_002665"
     CDS             159..1286
                     /locus_tag="KPG09_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBG0967262.1"
                     /translation="MIIPKLEPNDRKREFNKYSSEQINKVVYNYLFKNNMSNRKIDDE
                     ILDLSSKQSKGYQSMGIVHHLGINKNHKGIFTGYTIDKAIEVLKEDEIHFKQIINILL
                     EIRNINEYPIIVDSWELLDEKIALKTIDKSTLKHNGTGVPIEIRYFFEINNLGKNEKK
                     NVNFKYNDNYYSAVIEMDKLENPRSRIIWKSKFRDLLRSIFSEYYEKFLENKIEGKTN
                     LPKIRFIKLDSNNFNIEFIFTAEIEDDVLEEISQDNPLNINTINIELETEIRKEGKVK
                     YVYGKQYERNPRNRIEAIKYHGTKCIVCGFDFEKTYGDRGKGYIEIHHIKPLSSVGEE
                     SNINPKTDLVPICSNCHRMIHRKKDNVLSIDDLKQIIKDSK"
     gene            1766..2413
                     /gene="cas5"
                     /locus_tag="KPG09_002666"
     CDS             1766..2413
                     /gene="cas5"
                     /locus_tag="KPG09_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBG0967263.1"
                     /translation="MILNKIEIIGDMAHFKIPMHSKIQRTYEIPPISTVVGILKNIYG
                     EEIDDFILGYVIYHDGTFKDLQTIYKEINPNVKTLTDSDRFQTDVCPIEYLINPKLII
                     YTNIDKNIEFNEPLCLGKTNCLAKVLSLSEIEVNLIDKKAIGYNQYTDINIGDGMIKR
                     INTLTEYNEKKGYYDIYSSMVRENDEFEYGKYYDEDEQQNIFLWKWKKGGVIKCI"
     gene            2404..3168
                     /gene="cas6"
                     /locus_tag="KPG09_002667"
     CDS             2404..3168
                     /gene="cas6"
                     /locus_tag="KPG09_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG0967264.1"
                     /translation="MHLVRRHLILTVDNEVVLDYNYQYELMKRIYEAIEINDKRKALS
                     LHNEGYKVDKKVFKLFNYTIMFENAKYLKEGIHLNPQTKIKLILSGYDDILNNIIKGF
                     IKCKVFKLNNIEFKVSDIEEDSKKNFNNITLYKVRSPIVASLYDLKSRKQVYLNPMQE
                     EFYKALHDNLGNKYKLIHNKEYTGELYFDIEDVLAVKKKYITNIKGKGFIIGYTDFEI
                     FVQANKDMQEVIYYCGLGEKNSIGMGLLTYITSRRA"
     gene            3170..4336
                     /locus_tag="KPG09_002668"
     CDS             3170..4336
                     /locus_tag="KPG09_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967265.1"
                     /translation="MDRSYNVFVDNGLYVLAYYLNKDINDITYQDIENSIDLMSDKIE
                     EFVSCEKYSNLKSMCFSNSTLTQPKGKVTLNEKLKGFVKNQGDEYCSLCGQYKAKVKI
                     EDKEYNIGRSYMPNLVANTFYNFSNNLQGLNVCPYCLVLTMYSILNCRVSRYAFLYNS
                     TSSEFMEDYTFHIQKENLTDVELGAKKEKEKYSIVKLLESLVCKYNLFNGDIEQYMFN
                     NSGQSQDIQVNRIKNKYVNLLIKLQEKALLSNFEKLHLDRYILNGTLESNYLREVYRV
                     KKEEKMDEKEQKNFDELVIFLNREVNVLKKVKIDIIKKLTDKLKTSDLDIDKIYKELK
                     MLGKFYAYEDWLVNLNEMYYEKTGEKLFEAEDYIELDDIRSFRQIKNLILVSLI"
     gene            4357..5427
                     /locus_tag="KPG09_002669"
     CDS             4357..5427
                     /locus_tag="KPG09_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HBG0967266.1"
                     /translation="MENKNSNRMLNIGYAIEVLCGRLNGSSSEVEKKNNSYKNSTYTK
                     KIGSRSRISSQCQKYNQKLFMEQVCGYEQNVKTKDGKQVKLVPNPFKYATDDIFGFMM
                     AEKLSIEEDVYKELSDEEKEIYKKNKKKYEVNITKKRKSRWQMNSLINVSNRKVEWEW
                     NVCGTTGDSMPYQVEVYSGVHTNIANLNIGKIGEYDISDIASEHRDYTTLEAKSIGVE
                     KLDKEEKFKRIEALLKSLEYLSVEGNKTNHLTDTKPKFVILGEYSWGNNVFQGIIKEN
                     GLDVEMLKESIEQNEEFRKSDIWIGINKFYDDTFNEIEVNNEKEQIKKYLEEEFEEYD
                     FMKISNVHQAFESYLKYLKETL"
     gene            5438..7423
                     /locus_tag="KPG09_002670"
     CDS             5438..7423
                     /locus_tag="KPG09_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG0967267.1"
                     /translation="MNNLYEYKAKSDGTSIDKHNEDMNYIMNQLVSIYNISNDMKNDL
                     YEAIVCHDVGKVVKSFQDNIESSTRRVRHELLSASIKYLNRGQKLAILTHHKDIKFLE
                     GQITNSIYEIELKELKELLDIEVEDIRPFIKEISRARNDDTRKLENILLKGYLQYCDH
                     IASAGIKSIDKGFNSIKDFSIPKGVSYNSIQEKVLNMDKNEDIMIMAMTGLGKTATSL
                     YWSNLVQNEYKSKRVYYILPYTASINSLYENLYNRNISVSMLHSKAPYFLNKISDEED
                     IKQLYQIYKKSVKQINVCTIFQLVKSVFSCKRFEMLLSQLKDSIFIVDEIHCFDTRVL
                     CYTLEFLKYLKENLNVKICIMSASIPTCLKKLIQERLQISTIIEPDSKDLIFRHRLNR
                     LSEGILDSIHKIENDLMQGKRVLICVNSVDLSQELYGRLQKYNPKLIHGRFNTRDREQ
                     AEKGIEESFLLIGTQSIEVSLDISYDVLYTELAPLDSLLQRFGRVNRRGDNAELSNIY
                     IYDYDRKLNIYDEDIINRTDIVISDIIHNDNSIVDESKVPSYLDNVYLDIDMDKYNKH
                     KVALEIIMKNMRLGVWNKSANNDMLNNDTISVVPECLLEEYKLYIDKKMYLEASSLFV
                     NVSVKRYNYNKKMFYECGDIVVTSYSYNDKLGLIFDC"
     repeat_region   7676..8298
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=7676..7704
                     /rpt_unit_seq="gtttaaattacactaagttagttataaat"
     gene            8743..10299
                     /locus_tag="KPG09_002671"
                     /pseudo
     CDS             8743..10299
                     /locus_tag="KPG09_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444896.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            11477..12691
                     /locus_tag="KPG09_002672"
     CDS             11477..12691
                     /locus_tag="KPG09_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HBG0967268.1"
                     /translation="MGEKMQSVICKIFDNKLLISNDLSEQDDVLYEVMDKFIDDIEYI
                     SKLGDEQIKSMLMEVNKFEEDSTEFELYMKNLCYLKQSYFIDTNDKSEINSIDIDILK
                     NVKIEDLVDITNIKLERNLNINIEHISKAVNKIYEENEKWKEYNIQNTMSKYIGIPES
                     EVIFISNLSQYVSFLSELDNAGDYVSRGQKDCMYELLPSLHRKYKKDYGIHSDEYEEA
                     FKQKIIYYDKEIKNRSSEELRAEGQHFGLPTEYLDFTEAHLISLLFAIEDYKYTKQHS
                     IVYFIDSISYNTDTIKRSEKLVNYSDTSAIDNCSKFSSRSFFIKVGNSNERIHFQKGC
                     FLKVSSEDKGEFKEKLKKYCKVVVINKDAKKEILKELFNLGITFENIYPDKDNVVKSI
                     KFYYDEIVGGNL"
     gene            12691..13983
                     /locus_tag="KPG09_002673"
     CDS             12691..13983
                     /locus_tag="KPG09_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBG0967269.1"
                     /translation="MREQFKNSIKNIKSNRPLVSVQDIVNFIDKGEKYLKLSIDEIDE
                     MEILQGIVMSPDYQRTYKSSNKEESSIIESLLIGIPIPEIFLVKIGVNDMQIRHVMDG
                     QHRLNAIYRYVKNKYPLKNLDILGNDPLYDNKRFSELEKKDKIKILGSHLSILEFDGF
                     DNPEIEIELFKRYNRNTKPLEMQEIEMATYFSETSKYISKFINTLIKDKYKDEIDKSE
                     YKYANYKSDLLRIYNITKSRNDKQKNHQEICIIFSILENGLQENIRDGVTASKKFLEY
                     KSIQYKNNESEKLELLKDKFDEFNRMILKISEKIEYPFSTSLISDDKQRQSKYLIGVS
                     IVLASICYYYDIDLENNYLIQDIKKIISCSPIADIEYKASSTNMKNMILYLFIKNKIQ
                     DNKFESLNLKRDKIKEINTLVMNNDGYLNEQTRLLEIY"
BASE COUNT         6020 a         1248 c         2225 g         4496 t
ORIGIN      
        1 ttaataaaga ggggataagt atgccctctt ttatttttat gcaatcaatg acattgtagg
       61 atttacgtat tttgataaat agcacaatat attttaataa taaatatatg atacaataat
      121 tttattaaaa tctaaaataa tcaattgagg aggagaatat gataatacct aagttagagc
      181 ctaatgatag aaaaagagag tttaataaat actcatcaga gcaaataaac aaagtagttt
      241 ataattattt atttaaaaat aatatgagta atagaaagat agatgatgag atactagatt
      301 tatcatcaaa acaaagtaaa ggttatcaat caatgggtat agtgcatcat ttaggaataa
      361 ataaaaatca taaaggtatt tttacaggat acacaataga taaagcaatt gaagttctta
      421 aggaagatga gatacatttc aagcagatta ttaatatact tttagaaatt agaaatataa
      481 atgagtatcc tataatagtt gactcttggg agttattaga tgagaaaata gctttaaaaa
      541 caattgataa atctacatta aaacataatg ggacaggagt tccaatagaa ataagatatt
      601 tttttgagat aaataattta ggaaagaatg aaaagaaaaa tgtgaatttt aaatataatg
      661 ataattatta tagtgcagtt atagagatgg ataaattaga gaatccaaga agtagaataa
      721 tttggaaatc taagtttaga gatttactaa gaagtatatt ttctgagtat tatgagaaat
      781 tcttagaaaa taaaatagaa ggaaaaacaa atttacctaa aataagattc ataaaacttg
      841 atagtaataa ttttaacata gaattcattt ttactgctga aatagaagac gatgtattag
      901 aggagataag tcaagataat cctttgaaca ttaatactat taatatagaa ttagaaactg
      961 aaattagaaa agaagggaaa gttaaatatg tttatgggaa acagtatgag cgtaacccca
     1021 gaaatagaat agaagcaata aaatatcatg gcacaaagtg tattgtatgt gggtttgact
     1081 tcgaaaaaac gtatggagat agaggtaagg gatacataga aattcatcat attaaaccat
     1141 taagttctgt tggagaagaa agcaatataa atcccaaaac agatttggtg ccaatatgtt
     1201 caaattgtca tagaatgatt cacaggaaga aagataatgt tttgagtatt gatgatttga
     1261 agcaaattat aaaagatagt aaataatgct tatgtgtggc agagatgtat aaagtatata
     1321 ttctaaacta gttaacaaca aatactaata taaaaaatca aaaaatttat taggaaaagg
     1381 tagttgctaa agtataacag ctacttttat aaattaagta tttgaggtat attagtctaa
     1441 ccgattaggt atatcttaag ttattgatga aaattaatta agactaacat gtatgatgaa
     1501 tccatagaaa tgtattttta ttgttaaata aatgaacata catagaaata ttttagaaaa
     1561 ttacaagtac tatattgaaa gtttgaagga gaaccttgat ttaaggtaag tgtaaagttt
     1621 ttagaaactt cactaaaata atctaattaa gttatatatt tgttactttt aggaaaataa
     1681 tggtaaaaat taaaggtttt tataaatgaa tgtctaattt tataaataaa atggagtatt
     1741 ttaactaaat atttgaggag ataaaatgat attaaataaa atcgaaataa ttggagatat
     1801 ggctcacttt aaaattccta tgcatagcaa gatacaaaga acatatgaaa ttccaccaat
     1861 atcaacagtt gtcggaattt taaaaaatat atatggtgaa gaaatagatg attttatatt
     1921 aggatatgtt atatatcatg atggtacatt taaagattta cagactatat ataaagaaat
     1981 caatccaaat gtaaaaacat tgacagatag cgatagattt caaactgatg tatgtcccat
     2041 tgaataccta attaatccta aacttatcat atatactaac atagataaaa atatagaatt
     2101 taatgagccg ctatgtttag gtaagactaa ttgtttagcg aaggtattat ctttaagtga
     2161 aattgaagta aatctaatag ataaaaaagc tattggatac aatcaatata cagatattaa
     2221 tattggagat ggaatgataa aaagaataaa tacattgaca gagtataatg aaaaaaaagg
     2281 ttattatgat atatattctt caatggtaag agaaaatgat gagtttgaat atggtaaata
     2341 ttatgatgaa gatgagcaac agaatatttt tttatggaaa tggaaaaaag ggggagtaat
     2401 taaatgcatt tagtaagaag acatttgata ctcacagtgg ataatgaagt tgtattagat
     2461 tacaattacc agtatgagct aatgaaaagg atatatgaag ctatagaaat taatgataaa
     2521 agaaaagcac taagtcttca caatgaaggc tataaggtgg ataagaaagt atttaaatta
     2581 tttaattaca caattatgtt tgaaaatgct aagtatttaa aagagggaat acatttaaat
     2641 ccccaaacaa aaatcaaatt aattttaagt ggatatgatg atatattgaa taatattata
     2701 aaaggtttta ttaaatgtaa agtatttaaa ttgaataata tagagtttaa agtttcagat
     2761 atagaagaag attcaaagaa aaattttaat aatataacac tttataaagt aaggtcacct
     2821 attgtagcaa gtttatatga tttaaaaagt aggaaacaag tatacttaaa tccaatgcaa
     2881 gaagagtttt ataaagcatt acatgataat ttaggaaata agtataagct tatacataat
     2941 aaagaatata ctggtgaact atactttgat attgaggatg tattagcagt aaaaaagaaa
     3001 tatataacta atataaaagg aaaaggattt ataattggat atacagattt tgaaatattt
     3061 gtacaagcta ataaagatat gcaagaagta atttattatt gtggattagg tgaaaaaaat
     3121 agtataggaa tggggttact aacttatata acttctagga gggcttaata tggatagaag
     3181 ttacaatgta tttgttgata atggtctata tgtgttggct tattatctga acaaagatat
     3241 taatgatata acttatcaag atatagagaa tagtatagat ttaatgagtg ataaaattga
     3301 agaatttgta agttgcgaaa aatactctaa tttaaaaagt atgtgcttta gcaattctac
     3361 gctaactcaa ccaaaaggaa aagttacttt aaatgagaag ttaaaaggtt ttgttaaaaa
     3421 tcaaggagat gaatattgta gtttatgtgg tcaatataag gccaaagtca aaattgaaga
     3481 taaagaatac aatataggga gaagttatat gcctaatttg gttgcaaata cattttataa
     3541 tttctccaat aatttgcaag gtttgaatgt atgtccatat tgtttagtat tgactatgta
     3601 ttcaattctg aattgtagag taagtaggta tgctttttta tacaattcta catcaagcga
     3661 atttatggaa gattatactt tccatattca aaaagaaaat ttaactgatg tagagttagg
     3721 agcaaagaaa gaaaaagaaa agtattcaat tgtaaaatta ttagaaagtc tagtgtgtaa
     3781 atataactta tttaatggtg atatagagca atatatgttt aataactctg gtcaatctca
     3841 ggatattcag gtaaatagaa taaaaaataa atatgtgaat ttattaataa aattacagga
     3901 aaaagcacta ctatcaaact ttgaaaagtt acatttggat aggtatatct tgaatggtac
     3961 tttagaaagc aattatctca gagaagtata tagggtaaaa aaggaagaaa aaatggatga
     4021 gaaagaacaa aagaattttg atgaacttgt tattttttta aacagggagg taaatgtatt
     4081 gaaaaaagtc aaaatagata taataaaaaa attaacggat aaattaaaaa ctagtgactt
     4141 agatatagac aaaatatata aggagcttaa aatgttagga aaattttatg catatgagga
     4201 ctggttagtt aacttaaatg aaatgtatta tgaaaaaact ggagaaaaac tatttgaagc
     4261 agaggattat attgagttgg atgatataag aagctttaga cagattaaaa atttaatatt
     4321 agtaagttta atttaataat attatggagg gttaaaatgg aaaataaaaa ttcaaataga
     4381 atgttaaata taggctatgc aatagaagtt ttatgtgggc gattaaatgg aagtagtagt
     4441 gaagtagaaa agaaaaataa ttcatataag aattctactt atactaaaaa aatagggagc
     4501 agaagtagaa tctcaagtca atgtcaaaaa tacaatcaaa agctatttat ggaacaagtt
     4561 tgtggttatg aacaaaatgt taaaactaaa gatgggaaac aagtaaaatt ggttccaaac
     4621 ccatttaaat atgcgacaga tgatatattt gggtttatga tggcggaaaa attatctata
     4681 gaagaagatg tatacaaaga attatcagat gaagaaaaag aaatatataa aaaaaataaa
     4741 aagaaatatg aagtaaatat aactaaaaaa agaaagtcaa gatggcaaat gaactcttta
     4801 ataaatgtat caaatagaaa agttgaatgg gagtggaatg tttgtggaac tacaggagat
     4861 agtatgccat atcaagttga ggtatattca ggggtacata caaatatagc aaatcttaat
     4921 ataggaaaga taggagaata tgatatatct gatatagcaa gtgagcatag agattataca
     4981 actttagaag ctaaatcaat tggagttgaa aagttagata aagaagaaaa gtttaaaaga
     5041 atagaagctc tcctaaaatc actagaatac ttaagtgtag aaggtaataa gacaaatcat
     5101 ctaactgata caaaacccaa atttgttata ttgggagagt attcatgggg aaataatgta
     5161 tttcagggta ttatcaaaga aaatggctta gatgtagaga tgttaaaaga atctatagaa
     5221 caaaatgaag aatttagaaa aagcgatata tggattggaa taaataaatt ttatgatgat
     5281 acatttaatg agatagaagt taataatgaa aaggaacaaa taaagaagta tttagaagaa
     5341 gagtttgaag agtatgattt tatgaaaata tcaaatgtac atcaagcttt tgaaagttac
     5401 ttaaaatatt taaaggagac tttataaagg atatatcatg aataatttat atgaatataa
     5461 agctaaatca gatgggacaa gtatagataa gcataatgaa gatatgaatt acataatgaa
     5521 tcaattagtt agcatatata atatatctaa tgatatgaaa aatgatttat atgaggcaat
     5581 tgtatgtcat gatgtaggga aagtagtaaa aagctttcaa gataatattg aaagtagcac
     5641 aagaagagtt agacatgagt tattatcagc aagtattaag tatttaaata gaggtcagaa
     5701 gttagctata ttaacccacc ataaagatat aaagtttcta gagggtcaaa taacaaattc
     5761 tatttatgaa attgaattaa aggaattaaa ggaactatta gatattgaag tagaagatat
     5821 aagaccattt ataaaggaaa tatcaagagc aagaaatgat gatactagaa aacttgaaaa
     5881 tatattatta aaaggttact tgcaatattg tgaccatata gcttctgcgg gaattaaaag
     5941 tatagataaa ggatttaatt ctattaaaga ttttagtata ccaaagggtg taagttataa
     6001 ttcaatacaa gaaaaagttt taaatatgga taaaaatgaa gatataatga taatggctat
     6061 gacaggtctt ggaaaaacag caacatcatt gtattggagt aacctagttc aaaatgaata
     6121 taaaagtaag agagtgtatt atatacttcc ttatacagca agcataaatt ctttatatga
     6181 aaatttatat aatagaaata taagcgtaag tatgttacac agtaaagctc catatttttt
     6241 gaataaaatt agtgatgaag aagatataaa acaactttac cagatataca aaaagagtgt
     6301 aaaacaaatt aatgtatgta ctatatttca attagtaaag tcagtatttt cttgtaagag
     6361 atttgaaatg ctactatcac aattaaaaga tagtatattt atagttgatg aaatacattg
     6421 ttttgataca agagtattgt gttatacatt ggaattttta aaatatttaa aagaaaactt
     6481 aaatgtaaaa atatgtataa tgagtgcaag tataccaact tgcttaaaaa agttaataca
     6541 ggaaagattg caaataagta caattattga accagatagt aaagatttaa tatttagaca
     6601 tagattgaat agattaagtg aaggaatact tgatagcata cataaaatag aaaatgattt
     6661 aatgcaagga aaaagagtat taatatgtgt taattcagta gatttatctc aagagttata
     6721 tggaaggctt caaaaatata atcctaaatt aatacatggt cgatttaata ctagagatag
     6781 agagcaggca gaaaaaggga tagaagaaag cttcttattg ataggtactc aaagtataga
     6841 ggtaagttta gatataagtt atgatgttct ttatacagag ttagctcctt tagattcatt
     6901 gttacaaagg tttggtagag taaatagaag gggagataat gctgagttga gcaatattta
     6961 tatatatgat tatgatagaa agcttaatat atatgatgaa gatataataa ataggactga
     7021 tatagtaata tcagatataa tacataatga taattctata gttgatgaga gtaaagttcc
     7081 atcttattta gataatgtct atttagatat agatatggat aaatataata aacataaagt
     7141 tgctttagag attataatga aaaatatgag gttaggggta tggaataaat ctgctaataa
     7201 tgatatgtta aataatgata ctattagtgt tgtgcctgaa tgtcttttag aagagtacaa
     7261 gttgtatatt gataaaaaaa tgtatttaga agcgagttca ttatttgtaa atgtatctgt
     7321 aaaaagatac aattataata aaaagatgtt ttatgaatgt ggagatatag ttgttacaag
     7381 ttatagttat aatgataaac taggtctaat atttgattgt taagagaatt atagtataac
     7441 taattaaaat attttattga gaatttgtta taatttatat cgactacaaa ttttatatgt
     7501 tacaatagaa taattttgca gtgagcgaaa aatatgtgta aatggctgtg agttagtgat
     7561 ggcaaggttt ataactgaat ttttgttttt agctaaaaac actactggtg cctcactgca
     7621 aattaactat tttaataacc ctacaagtgt tgtaattact atagttttat ttggggttta
     7681 aattacacta agttagttat aaatattccg gaaagtactt ttgctatttg attcattcta
     7741 cccgtttaaa ttacactaag ttagttataa atttcactaa ttttagttac aactattaca
     7801 agtccagccg tttaaattac actaagttag ttataaatta tgtgtaatgt attttaatgt
     7861 tttctagcaa caatgtttaa attacactaa attagttata aattttataa attctatgaa
     7921 taattttaaa gagaggtttc gtttaaatta cactaagtta gttataaatt gtacatttga
     7981 catagaagct tcaaaatcca tcccagttta aattacacta agttagttat aaatgtaaca
     8041 gcacgaatga tggcacagac ctatctgcaa tgtttaaatt acactaagtt agttataaat
     8101 agaagtacac ttacaagtaa gataaaagac ccaggtacag tttaaattac actaagttag
     8161 ttataaattc tgtagctata tgtggaagta attttagtag agaggtttaa attacactaa
     8221 gttagttata aattattgaa ggggtgagag ttgtgactag gcactacaag tttagattac
     8281 actaaattag ttgtaaattt acttattcat taagaaactt aactgagagt ttgatttata
     8341 ttattgaatt agcaagagtt ttagaaagta gaaaattata ccccaataat accccaacca
     8401 caaaaataaa taataaaaaa tatccaaaaa aattagtata acatcataac taaatagcta
     8461 tatacctaca cttacgaaaa ccaacaaaac aatcaaataa ccacttgtag actcccctcg
     8521 tctccaccaa ttttgaaaat gaaaataata gaataaaagg aattacttat gtttggacta
     8581 agtaattcct tttattttgc ttaaaagcac acaattagca cacaactaga aggttatata
     8641 aataaaaagg ctgtcgcact agaaaatagt gctacgctct tttttgtata aaaaaataaa
     8701 tcccaagttc aaaagaacct gggattcttg tataataact gtatgctaaa acaaaatact
     8761 acacaaattg attatactac ttatggtgat aattatcaat ttaagctacc actaaatatt
     8821 gattacatga ttccaaaaga tgattctgta aggttattaa gttaaattat tgaggagatg
     8881 aatttagaaa aattatataa gacttattct cgaattagat gaaatagagt aatccataga
     8941 caatttttga aagttataat atatacagat atgaactgta tttattcttc gagagaaata
     9001 gaaagaacat gcaaaagaga tattaatttt atgtaccttc taggcgaaac ctccactcca
     9061 gaccattcaa caattgcaaa atttcgcagt atacattttt aactaatagg caaaagcttg
     9121 attgcacagt ttacaaactt tcttggagat aataatgaaa tttcaaaaga cacaatattt
     9181 attgatggta caaaaataga gtcatctgct aatagatata cttttgtttg gaaaaaagct
     9241 gtaaataaaa atatggctaa aatggtgaat aatatatctg acttcatatt gaaatgtgag
     9301 attgatgttg gcattaaaat agtttatgaa gataaaatta aaatctatca tttaaagaaa
     9361 ctaaaaaaaa tatcaaaaga agcaaatgta gcatttattc atggaaaagg aaatagaaaa
     9421 tctactaaaa accttcatat aatgggtgaa cgaaatagtt gttctaaaac agataacgat
     9481 gaaactttta tgagaatgaa agaagatcat atgaaaaatg ggcaactgaa accagcatat
     9541 aatatacaat ttggtgtaga ttaagaatat atagtttggc taaattcagg aaatcaaact
     9601 gctgatacta caacattaat accattttta aaatctatag aggaatatac taagtttaaa
     9661 tatcctaaag taatagctga tggtggatat gaaagtgaat aaaactatgc atttttagag
     9721 cataataatc agttatcttt cataaaacct gcaaattatg aaattattaa aactagaaaa
     9781 tataaaactt atattggatg tattgacaat atggattatg aagaagaaaa taattattat
     9841 atttgtacaa atgagaaaaa actatttgct aataaaataa taaataggaa atcaaaaaca
     9901 ggatataaaa gtgaaataac atgttacatt tgtgaagagt gtagtaattg tcaatataaa
     9961 agcaattgta ttaagagaag taatagtaag gttcctctta aaaataggac taaaaactta
    10021 caagtatcta agtcgtttca tgaaaaaaga aaagaaaact taaagagaat tatgagcctc
    10081 gagggggatg aacttagagt gaatagaagt atacaagcag aaggagcttt cgctcaagta
    10141 aaataaaata tgaaacttac aagattttta agccaaggta aagaaaatgt tttataagga
    10201 tatattattg tggcgatagc ttataacgtt tctagactac acaataaaat agtaaaaaat
    10261 aaacgtggaa tatatcttta tcatttaaaa aaatattcta aaattattat ttataatcaa
    10321 atattatctg aaaaaatggt atttttatat ctttatttgt catgcataat tttacttaga
    10381 acagaaactt tatataaaac aattgaaaat atacctatta agataattag aaaagagctc
    10441 tcacagaaca aatttaattt gctcatgcaa cagccccata ttgatataca tttaggttgt
    10501 ataattatac atttcaatat tacatgttca ttaggataat agttagtaaa tgatatgatt
    10561 gagagaatag atataataaa ctaatctagt cctgtattgc aatgtttatt aaagaatata
    10621 tttttaaaga tccaagtact gcatctcttc cagaattgag ttggttaata gttttttgat
    10681 gctatgcaat ttataataaa aagtataatg atttagttga tgaatcatca gaatttatac
    10741 cctcaaagat taatcagatt gcaataaaaa taatgatata ttctatagtt ataatatcta
    10801 ttttctttgc aacccctcat acaatgaata taaatatatc aaacttggat atgggtataa
    10861 ttctcatttc agttttattt atacagtttt tatctaaatt aattttgtat gtatattttg
    10921 ataaaaggga gatatgtaac taagtggtaa tattggtgaa aacaaaaatt aaagaatata
    10981 gaaaaaaact ccttatgaca caaaatgaac ttgctaaatc agttagggta aggtgtgaga
    11041 ctattgtaca cttggaaaat ataatccttc tttaaagtta gtgatagata ttgctaaagt
    11101 attcaatact acagtagaaa atttgtctga atttattgaa gaaggatagt ggatagatta
    11161 taaatagtta taagatggtt aatgaaatat agttacttaa ttattaagtg tgttagaaat
    11221 atttatatgt aacacaatgt gtagttaaga ttaccttatc tcatctaaga ctttaatttt
    11281 tcttcccata aagagggtat atcaattttc tagtgtggta gcccattttt tattgattta
    11341 aatttataat atatattaat agatgatact taaaaatttt ataaaatatc taagttgtca
    11401 taaataaaac gatatatcta accgaatatg gtaaaatttg ttataatata gataagttag
    11461 agtaaaaata attttaatgg gggagaaaat gcagtcagta atatgtaaaa tttttgataa
    11521 taaattacta attagtaatg atttaagtga gcaggatgat gtattatatg aggtaatgga
    11581 taaatttata gatgatattg aatatatttc aaaattagga gatgagcaaa ttaaaagtat
    11641 gcttatggag gtaaataaat ttgaagaaga ttcaactgaa tttgaactat atatgaaaaa
    11701 cttatgctat ttaaagcaat catattttat agatactaat gataaaagtg aaataaattc
    11761 tatagatata gatatattaa aaaatgttaa aatagaggac ttagttgata taactaatat
    11821 taaattagaa cgaaatttaa atataaatat agaacatata tctaaagctg taaataaaat
    11881 atatgaagaa aatgaaaagt ggaaagagta taatatacaa aatactatgt ctaaatatat
    11941 aggtatacct gaatcagaag ttatatttat atcaaatttg agtcagtatg tttcattttt
    12001 atctgaacta gataacgcag gcgactatgt atctagaggg caaaaagatt gtatgtatga
    12061 attattacca tcattacata gaaaatataa aaaagactat ggtatacatt cagatgaata
    12121 cgaggaagca ttcaaacaaa aaataattta ttatgataaa gaaataaaaa atagatcgag
    12181 tgaagaatta agagctgaag ggcaacattt tggactacca acagaatatt tagatttcac
    12241 agaagctcat ttaatatcat tattatttgc aatagaagac tataaatata caaaacagca
    12301 ttcaattgtg tactttatag actctatttc ttataatact gacactataa aaagatctga
    12361 gaagttagta aattattcag atacatctgc aatagataat tgtagtaaat tttcatcaag
    12421 atcatttttt ataaaggtag ggaattcaaa tgaaagaata cattttcaaa aaggatgttt
    12481 cctaaaagta tcatcagaag ataaaggcga atttaaggaa aaattaaaaa agtattgtaa
    12541 agtagtagtt attaataaag atgctaaaaa agaaatatta aaagaattat ttaatttagg
    12601 tattacattt gaaaatattt atccagataa agataatgta gttaaatcta taaaattcta
    12661 ttatgatgag atagtagggg gaaatttata atgagagaac aatttaaaaa ttctataaaa
    12721 aatattaaat caaacagacc tcttgtatca gtccaagata ttgtgaattt tattgataaa
    12781 ggagaaaagt acttgaaatt atcaatagat gaaatagatg aaatggaaat tttacaagga
    12841 atagttatga gtcctgatta tcaacgtaca tataaatcat caaacaagga agaaagttct
    12901 ataattgaat ctcttttaat tggaatacca atacctgaaa tatttcttgt aaaaataggt
    12961 gtaaatgata tgcaaataag acatgtaatg gatgggcagc atcgtttgaa tgctatatat
    13021 aggtatgtta agaataaata tccacttaaa aatttagata tattaggcaa tgacccacta
    13081 tatgataata aaaggttttc agaattagag aaaaaagata aaattaaaat attaggaagt
    13141 catttatcaa ttctagaatt tgatggtttt gataatccag aaatagaaat agaattattt
    13201 aaaagatata acagaaatac aaaaccatta gagatgcaag aaatcgagat ggcaacatat
    13261 ttttcagaaa ctagtaagta tataagtaaa tttataaata ctctaataaa agataaatat
    13321 aaggatgaaa tagataaatc agaatataaa tatgctaatt acaaatcaga tttacttaga
    13381 atatataaca taactaaatc tcgtaatgat aaacaaaaaa atcatcaaga aatatgtata
    13441 atatttagta ttttagaaaa tgggctacaa gaaaatatca gagatggagt tacagcctct
    13501 aagaaatttt tagagtataa gtcaattcag tataaaaata atgaaagtga aaaattagag
    13561 ttattaaaag ataagtttga tgagtttaat aggatgatac tgaaaatatc tgagaagata
    13621 gaatatccat tctcaacaag tttaatatct gatgataagc aaagacaaag taaatacttg
    13681 ataggagtat ctattgtgtt ggcttctatt tgttattatt atgatataga cttggaaaat
    13741 aattatttaa tacaagatat taaaaaaata atatcttgtt cacccatagc agatatagaa
    13801 tataaggcgt cttcaactaa tatgaaaaat atgattctat acttatttat aaagaataaa
    13861 atacaagata ataaatttga atcattaaat ttaaaaagag ataaaattaa agaaataaat
    13921 actttagtaa tgaataatga tggttattta aatgaacaga caaggctatt agaaatatat
    13981 taacaagtt
//
LOCUS       DAELSY010000104        13876 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.104, whole genome shotgun sequence.
ACCESSION   DAELSY010000104 DAELSY010000000
VERSION     DAELSY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13876
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.104"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1582)
                     /locus_tag="KPG09_002674"
     CDS             complement(<1..1582)
                     /locus_tag="KPG09_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG0967270.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGNCTIDAG
                     VLYLDLLTNLERISDHSTN"
     gene            complement(1891..2097)
                     /locus_tag="KPG09_002675"
     CDS             complement(1891..2097)
                     /locus_tag="KPG09_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0967271.1"
                     /translation="MFKKKSGYTFTNFLNKVRVEKNKKYLLKKDLSILDVTVLIGFNS
                     QNYYSIIFKKFNNLTPIEYKNIYN"
     gene            2402..2593
                     /locus_tag="KPG09_002676"
     CDS             2402..2593
                     /locus_tag="KPG09_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967272.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            complement(2682..3494)
                     /locus_tag="KPG09_002677"
     CDS             complement(2682..3494)
                     /locus_tag="KPG09_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBG0967273.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            3916..5067
                     /locus_tag="KPG09_002678"
     CDS             3916..5067
                     /locus_tag="KPG09_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0967274.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDDDLDKMLELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TESLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            5185..5823
                     /locus_tag="KPG09_002679"
     CDS             5185..5823
                     /locus_tag="KPG09_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG0967275.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            5824..6846
                     /locus_tag="KPG09_002680"
     CDS             5824..6846
                     /locus_tag="KPG09_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBG0967276.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEYIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRGYDNFLCDFGCNCLNTTPVENLQAAIKAGRINYKELDR
                     IDIDEL"
     gene            6836..7456
                     /locus_tag="KPG09_002681"
     CDS             6836..7456
                     /locus_tag="KPG09_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967277.1"
                     /translation="MNYNSNKNKYLLKDINLEDIFIDKNIVLKFLGYGNRKAPDTISE
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGIALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(7580..8266)
                     /locus_tag="KPG09_002682"
     CDS             complement(7580..8266)
                     /locus_tag="KPG09_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBG0967278.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALMIGNSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(8280..8825)
                     /locus_tag="KPG09_002683"
     CDS             complement(8280..8825)
                     /locus_tag="KPG09_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0967279.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCIDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            9053..10300
                     /locus_tag="KPG09_002684"
     CDS             9053..10300
                     /locus_tag="KPG09_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG0967280.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSSGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            10387..12381
                     /locus_tag="KPG09_002685"
     CDS             10387..12381
                     /locus_tag="KPG09_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG0967281.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIKNTFSENTN
                     KTSEIEIYNKILKINTFPVEYKKNNTEYIVVLIKDITNEKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            12381..13703
                     /locus_tag="KPG09_002686"
     CDS             12381..13703
                     /locus_tag="KPG09_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG0967282.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
BASE COUNT         5479 a         1535 c         2122 g         4740 t
ORIGIN      
        1 tatttgttga gtggtcagaa attctttcta gattagtaag taagtcaaga taaagtacac
       61 cagcatcaat tgtacaattt ccattattta gtctaatcat atgtgaattt ctaaaagttt
      121 tttctaaata atttacttca ttttcaactt ctaaaacttt atttgctaac tcaaaatctt
      181 taacatctaa aactattaag gcatccttaa aattagtaat tgtcttattg tatatttcgt
      241 atatttctct tatcgcacta tcagaaaaac taatgttcat ttccttaaca ctcatggcca
      301 attcagctat attttctgca tggtcagaga ctctttcaat gtcatttaca gtattaaaaa
      361 gcaaatctgt tctaaagcgt tcatcgtctc taagaggttc tttagaaagt tttaacaaat
      421 agttcaatat atctttctgt aacttattga caactttttc tgactcaaaa gctttattaa
      481 tatcttcatt gctcttattt aaaaatcctg caattgaatg ttccaaagag tttgtagctt
      541 tatgacccat tcttacaact tcttgtacag tattaccaag tgctatagat ggagtgctaa
      601 acatcctttc atctaagtac ttagtattat ttactatttc tgattcagtt tctttaatag
      661 gtataagtct atttgctaat ttaactatgt atttagaaaa tggtaataat attataacat
      721 ttgtaagatt aaataaagta tgtgcatttg ctatttgtct agctgtatca gttgggtcaa
      781 aatgagtaac tatggctgat ataggtttat ttaaaacaag cataaatatt attgtaccaa
      841 tgacattaaa acttaaatgc attacagctg cacgtcgagc atttctactt gcaccaacac
      901 ttgataaaag tgaagtaaca caagtaccta tattatctcc ataaagaata ggtaaagcag
      961 atgatagagg aagtattcct tgagaagcta gtgctattaa catacccata gatgcacttg
     1021 aactttgaac aataccagtt atggcaaatc ctgccaatat acctaataca ggatttttac
     1081 taaaatataa aagtgcatca gtgaatgctt tatattcagc aagtggagct actgcatctt
     1141 tcataaactc cattcctgta aataatatac caaaacctat aagtatctca gataaatgct
     1201 ttgttttttg atttgatgta aataaataga ataaaatacc tatacctaaa gcaattggtg
     1261 caattccttc aagattaaat gaaactaatt gagcagtaac tgtagtacct atatttgccc
     1321 ccattattac accaatagct tgacttagat tcattatacc tgcatttaca aatcctacaa
     1381 ccataacagt agtagcactt gaactttgta taatacctgt aacgactgta cctaccagta
     1441 cacccataac aacattactt gttaagagtt caataatttt ttttaatttg tctcctgctg
     1501 atttttgtaa accttctccc attaaactca ttccgtaaag aaaaagtcca agtccaccga
     1561 taaggctaat tactatattc acgatatttc ctcccttaag aaattaatag tttttttatc
     1621 tgaattataa actttattaa ctaaaattta ttgtttccat aatatagaat aacaaatttt
     1681 gtcaatagaa atattaatgt tttgttaagt atacgttaag agaatgttaa atttttaagt
     1741 tgttgttaat tttagaaaat ttatgatata tagcaattta aatacttgaa cttagatttt
     1801 tagtatttta tgtacaatca atttaacaaa gaattaacta taattgtaaa aaatatgtaa
     1861 aaaaatagca tccttttaga atgatatttt ttagttataa atatttttat attcaattgg
     1921 tgtaaggtta ttaaactttt taaaaattat actatagtaa ttttgactat tgaatccaat
     1981 tagtacagta acgtctaaaa tagacaagtc tttttttaat agatactttt tgtttttttc
     2041 aactctaact ttatttaaaa aattagtaaa agtatatcca ctttttttct taaacaaaga
     2101 aaaaaataac ttttattaag ttgtaattca ttacatatat tacttactgt tatatcttct
     2161 gagtagtttt tgtgaatata atctatagtt ttctttaagt tttctttaca taaaactaag
     2221 agttggtcta tgtttcattt tatcaaggca tatttattaa aaaatcaatc tctatttgtt
     2281 aaaataattt atgaatgatg ttagaaactg taatatattg gatataatat aataaacaga
     2341 taaactctgt attaagagga tatatagttt atcttaatta taggggaagt gaggatatat
     2401 tatggaaaga ttaatggaac tcatagaaag aaaattttta aaggatgtag aacttttaga
     2461 attagaagac atagaaaatg taatagaatg caaaatgaca cagccatata atgctcatta
     2521 taatgaatac ttagtaaaag taaaaaatgg agatgaagta gaagaatatt ttgtgtattt
     2581 aaaacataga taaacataaa agaggtttat atttatttgt atgattataa aataaataca
     2641 agcctctttt tttgataaat ataaaattat aaaattttta tttatttaat tttttcaaca
     2701 acattagtca atattgaatc taaatcatca ctaatttctg taactccatt atgagtaata
     2761 tctataattt ctttagtcat aggaagttct cctaaaagct ctaagtctaa atcatctaaa
     2821 aacttttcag tagattctcc ttcaaataac ttgatttttt tagaacaatc aggacattgg
     2881 atataactca tattttcaat aactccaagg acatttatgt tcattttttt agccatatta
     2941 acagcttttg atacaatcat agataccaaa tcttgaggta ctgaaaccat aactattcca
     3001 cttataggaa tagattgcat aaccgttaag gcaacatctc cagtaccagg aggcatatct
     3061 attaataaat agtctaattc ttcccaaagt acatcagtat aaaattgctt tacaactcct
     3121 ccaagtaaag gtcctctcca aacaacaggt tcattctcat catctatcat aaggtttata
     3181 gacattactt ttatgttatt tgagttttct acagggtaaa tataagagcc atcagaataa
     3241 gcttttacat tttttacacc cataagtcta ggaatacttg gtccagttat atctgaatct
     3301 aatataccaa ccttatatcc cattttgttt agcttattag caagtaaagc agttacagtt
     3361 gattttccaa ctccaccctt cccactcata acaccaatta tttttccaaa tttattgtta
     3421 gggttatttt caatagaaca atttgattga ctattacaat ttccctttga tggacatgaa
     3481 ttacaatctg ccatatctat tacctccaaa ctttaatatt tattagatta tttgtaacct
     3541 acatatacta ttttagtaca attgaaccat aaagtcatta tttaaaagca acttagaaga
     3601 ttttctattg atttattcat agactatagc aaaaagaata caataaaaaa attataacat
     3661 tcttgaaaaa gtgattattt caatttagtt atagaactta gtcatataaa tatatagaaa
     3721 atattttaat atattataca taacgggtta ttttataagt ttaactataa attaggttta
     3781 tattttataa aaatttgtta aaaaaaacaa ttaaaaattg taaagaaaaa agatataatt
     3841 agaactgtag taataaatgt tttattatag ataatagagt tttaagtatc ttaaataata
     3901 tttggaggaa tatgaatgga attaagacca agaagtgcaa aattagaaga atttgataaa
     3961 gtaatagaat taataaacta tgtatttaga atatcaagaa atcataagcc aactatgatg
     4021 gaagaatttc ctcttttact aagtaaaaat aatatagaaa atatgattat aatatcagaa
     4081 gatgataaag tggtttctga tgtaaactat ctaattcaag atgtatctat tcaaggaaat
     4141 agattgaagg ttgcagctat aggtggagta tgtactcatc cagattatga aaagagaggc
     4201 tattcatcta agatattgga taaagtagaa gaaaaaatgt tttatgatgg tgtagatatt
     4261 gtaattatat caggaacaag aagtctatac agtagaagaa attgtagttt ggtaaagagt
     4321 ttttataaat atacaataaa acctgaagat gtaaaaatag catatgaaat agttgaattt
     4381 gatgaaacta attttgaaaa agatgatgat ttagataaaa tgcttgaact atataatcaa
     4441 aatagtacta gatttataag aacgagagat gagtttcaaa aactattgca tgcagcaact
     4501 attgcctggg gaccaattgg ttacaagaag gtatttataa aggaaaataa taatataata
     4561 ggatatttga taataagaac aataaaaaaa gaagattcta ctgtaggaga agttgctgaa
     4621 atagggttaa actctgtaaa tgttgaaaat atattgaaat atgttgcaaa taaatttgga
     4681 ttagaatatt taaattataa agtacatgtg aaaaatctta aagaccagtt gaaatgtaat
     4741 gggacagaat ctttggacta tcaacaagga actatgaaaa taataaattt tactaagttg
     4801 tgtgatagtt taagaagtta ttttagtcaa tatgtagatt ttgaattact aaaatacatg
     4861 gaatttaaac aagttgaaaa taaatatatt attaaataca aagaagaaga acttgtaata
     4921 gaaaatttgg ataagcttaa taaactgttt tttgaaaaaa atgaggaaca gtataatgaa
     4981 tttaaacatc taaaaaatat atatgaattt gctacaaagg catttcctgt agattttcca
     5041 tggactgcaa atttaaatta tcaatagttt tagatgatat taaataaaat tgattgatac
     5101 ctagagttag tccttgttaa ataagagaaa ttattggata ttgaaaaagc aattttgtaa
     5161 gtaattagaa aggatgagta ttatatggac atacttgatg atatagcaga atgtatatta
     5221 aatatgggtg tagataatat agagaattta gtaaaaattg ctatagataa aaatattgat
     5281 gtggaagata tttatgaata tggtttaaac aaaggtatga taggagcttt agataagttt
     5341 gagaataagg aatactatct ttcagaagtt atagtatgca cagatgcatt aaataaaggt
     5401 attaatcttt tgaagggaac tggaaaagta aagaaaaaat caaagggagt aattctgatg
     5461 tctgtagtag aaggagatac tcatgagata ggaaaaaata ttgtaaaagt aatggtagaa
     5521 gccactggat ataaggttat tgacttaggt gtaaatagaa aaagtgaaga tataatagaa
     5581 gaagctataa aaaacaatgt agatataata ggcttatctt caatgatgac aactacaatg
     5641 gaaaatatga aatcggttat agacgagctt aatatgattg agatagataa aagaccaaaa
     5701 gtaataattg gtggaggacc tgtaagtatg gagtttgcag aagaaatagg agcagatgga
     5761 tacagttcaa atgctccaaa agcagttaaa ttaataaaca aacttatagg aggtgaagtt
     5821 taaatgtttt cagatatgac tttacttcag tatataaata gtgaagatag aggttttttt
     5881 ctgcctgata tgggtacaaa tggattgttc ttaataaata aaaaggcata tgaagtatat
     5941 gatagtgtag atttgcaatt agaattagca aagactatgg acaagtattt tgaatcagat
     6001 tttatatact cattctgtga tgggattaca ttttgtgaaa ctctgggttt agagacatta
     6061 agaccagatt atgattttcc aagtgtattg acacatacta taactgacag agataaatta
     6121 agaaaattag atgttccaga cccttatacg agtggacgta tgccactcaa tatagagagc
     6181 ttatcagtta tatcaaaaag tattaaaaaa ccactatatg aatcgataca aggtccattt
     6241 acacttgcag gtcaattagc tggagctact caacttttaa gatgtataat aactgataaa
     6301 aaatttgtag aagaattgtt ggaatttaca actgaattgg taagaagata tgctgtagct
     6361 gcaaataaag caggagcaaa atatatttca atagcagagc caacgtcagt aaccctaagt
     6421 aaagatagat ttgatgaata tatagtaaaa aatttaaata aaatctacaa tgaacttgat
     6481 tgttggaaag gtatgcatat atgtggagac accagagaat tattagataa tatgctaagt
     6541 tgtaatatag atgcagtaag tttagaccaa attttagact atgaagaaat tgctccaatg
     6601 attcctagag atatagtgtt gattggtaat ttagacccaa taaaacttct tggacgttct
     6661 aagccagata agattagaag agagacacta aaattgttaa agaagatgag aggatatgat
     6721 aactttttat gtgattttgg ctgtaactgc ttaaatacca ctccagtaga aaatttacaa
     6781 gcagctataa aagcaggaag aattaattat aaagaattag ataggattga tattgatgaa
     6841 ttataatagt aataaaaata aatatctttt aaaggatata aatttagagg atatttttat
     6901 agataagaat attgtgctaa agtttttggg gtatggaaat agaaaagctc cagatacaat
     6961 tagtgaaatc gttgaaagag aaataagaaa tataaatgat attttagata tcaagatata
     7021 tataagtgaa gttgatatta ataaaacacc taaagaatgt aaaaaagcat ttgctgtact
     7081 atatacaata ggggataaaa ttgatattaa gatgaatgac tatatggaaa attgcaatat
     7141 gatggcaggg atagcattgg ataaaatagg tatagtatgt ttagattata ttaatgagaa
     7201 aataaaaatg tatttaaaag gaaagtatga tagtcttaaa atatcgcatg aaatatatcc
     7261 aggagataaa gattttgaag tagagagaca aaaggatatt tataactatg taaaaaataa
     7321 atataatagt atagaaatag aaatcaatga ttatcatcaa ctatccccca taaaaagtgt
     7381 tgcaatgctt attttaatgg gagatgaaga aaatttagaa tcaagatgta gcaaatgtcc
     7441 tagaaaatgc ttttaactag ttttcaagac taagatttgt tttgtgtatg aattgcaata
     7501 atttttttgg tcattttatg tataaactaa aaaaattatt gcaaagtaat attctattta
     7561 atttgaacag acttacaaac tatagtagtt tctttaattc aataagtgta tttatttcat
     7621 aagttggtat atgattagta taatttatac tgttattgga atttaaccaa caagtatcta
     7681 ttcctgaatt tattccacct aagacatcag aggttaagct attcccaatc attaaggcag
     7741 aagatttatc atgacaatta aactttttga gagcgtattt aaatatttca ggatttggtt
     7801 ttgatatttt caactcttca gatacaacca ttccatcaat atatcctttt atttttgaat
     7861 tttcaagtct tttcttttga actgcagaaa ttccatttgt tattataaca atagtatact
     7921 tttttttaag atattgtaat atatcgattg cacctggaat taaaaatgta cattcaccta
     7981 aaaaatttaa gtacattttg ctaagagttt cagaatcaac atccaaagat attttatttg
     8041 caaaaagttc aaatctaagt aatttaagtt cattcaaagt tataatattt ttttcaaggt
     8101 cgagccaaag cttatcactt atatttctat atgactctat ataattctca aaattaaatt
     8161 ctaaatcaaa ctcagataga agttttttaa aagcatgtga ttcacttttc ttaaaatcaa
     8221 ataaagtatc atctgcatca aaaaatataa atttatatgt cttcatggtt tttactcctt
     8281 tacaagatga ctgcaaaaat tactttactt tcactatttg agtcattttg ccatttatgc
     8341 tttgttccta aaggaacttt tacactatct cctttggaca aagtaaattc atcatcatcc
     8401 ataaaaagtt taacttctcc ctccaaaaca tatgcaattt catctccatt atgagtaatc
     8461 ctatcaatac aagaagaagt atttggagct aaatccataa ttgcaaattc taaattacca
     8521 ctagagtctg gaattaaaat ctcatatata acttctttgc ttccaggtaa aataactttt
     8581 tttctattat catgtcttac tatcaagttt tttttatcta cttcagatac aaaaaatgta
     8641 aataaaggaa cgtttagaga agaggctata gattttaatg aatttacaga tggattagca
     8701 agacctcgtt ctatttggct caataatgat ggtgtaacat cagctagttt tgctaaatct
     8761 cgaatactta agttttgttt ttttcgaact tcagctattt tttcccctaa atttaaatca
     8821 ttcatataaa tatcctctct ttttaattta aaaaatatta acttaaaata atcaagtaaa
     8881 atgattatgt agtatgaaat tattataaca tatagaatta ggttttgaaa aaactatgtt
     8941 caatataatt aaataaatta aaatataatt gacaaagtgg atacagaata cttaaaatat
     9001 atttaaataa aattaagcaa aagatagtta atttatgata ggaggggaca ttatgcaaat
     9061 aacgactttt ggagctatat ttggtttatt aatagcaatt attttaatta taaaaaaatt
     9121 tcaagctgta tatagtttaa tgttaggagc atttattgga ggattagttg gaggagcaaa
     9181 tataacacaa actgtagatt ttatggcaaa tggagctatg aatatatcac catcaattct
     9241 tagagctttg gcatcaggtg tattagctgg aagtttaata aaaacaggag cagtagataa
     9301 aatatcagaa cagattgtaa aaatatttgg tgaaaagagg gctttatttt cgattgccat
     9361 ttcaactatg gtacttgctg gagtaggcgt aaatttggat gtatctataa taactgtagc
     9421 acctatagga ttatatatag gtagaaaatt aaattattct aaattgtcaa tactattagc
     9481 tatgttggga gggggtaaag caggtaatat aatatcacca aatccaaata ctatagcagt
     9541 agcagataat ttttctgtca atctctctag tgttatgatg gcaaatataa ttccagcaat
     9601 tattggagtt gttattacag ttattttagc aagtatatta ataaataaag gaaataaagt
     9661 acaatcatat gaaattttag agcaaagaga agatttacca agtttattta aatcattatg
     9721 tggtccaatt atagctatat ttctattgtt tttaggaaat gtatctccta tagttataga
     9781 cccaatgata gcccttccaa taggaggaat cgtaactcta attgtcacag gaaacttgaa
     9841 caattcaaga gaatatttag cttttggatt atctaagatg caaggggtat gcatattatt
     9901 acttggaaca gggactatcg caggaattat acagatgtct gaattacaac agagtacaat
     9961 aggtgcttta caatttttaa atatgccaca atttttattg gcaccagttt cgggtatact
    10021 tatgtcatta gcaacagcat catcaacagc tggtgctact atagcatcat ctacttttca
    10081 tgatgctata ataaatggtg ggctatcacc gatttcaggt gcatcaatag taaatgctgg
    10141 gtctagtgta ttcgagcaat taccacatgg ctcactattt catactagtt caggtagtat
    10201 aaacatggat ataggtgaaa gatttaagtt aattccatat gaagcattga taggaatagt
    10261 aatgactata atttcaacat ctattcaatt agttttataa taaatataaa tatttaatga
    10321 aaaaagtagg attttgttat taaatattga atttattata gtaaaaaaga agattggagt
    10381 tttaaagtga ataaaaaaaa gattgttatt atagggatta tttattcatt tttagtagta
    10441 ttttcactta caaatatgta tgtaaatatg gagtataatt taaatgtatt tgaatatatt
    10501 aagaagtcac ttccatttac agaagaagaa aaaaagtggt tagaaaaaca caagaatttg
    10561 atttacagct ctgaccaaag ttcacctcct ttgagatata agggtaagga agatggacag
    10621 tataagggaa ttattgttga tttaataaat tctctgtcaa ttcaaattgg tagagatttt
    10681 tattttaaac caaataattg gtggaaagaa tctttcgtaa attcaataga tgatagtata
    10741 aagttttttg acttgattcc atcaaaagaa agggcaaata aatttatatt tactgaccca
    10801 atatacactt tgagtgcaaa tatattgaaa gataaaaagt cccaagatat aaatagttac
    10861 atggatttaa aggggaaaac agtagcaatt ccagaaggtg attattctat aaatttttta
    10921 aagcaaaaaa ttcaagatat aaacatttta ttgactccag atataaagac tggagtcaac
    10981 catttaatgt ctggaaaagt agacgcagtt gttggtgatg aaccagtatt gcgatactat
    11041 ataaataatt atggactttc aaataaatac agtgttttaa gtaatcctat atatactaaa
    11101 aaagcagttc ttgctgtacc taaacaatat gaagagctgg ttagtatttt aaataaaggc
    11161 atttttaaac ttcagaaaaa tggagtgtat aaagatttaa agaaaaaatg gtattctacg
    11221 tataatgaag ttgatgatat attgtatgag cgaggaattg tgcctagtat ttatcttttt
    11281 ataggtataa ttttaatatc aatttatgtt ttttatagct atacatattt gttaaagata
    11341 gaaatcaaaa ggaaaacaga acaggtaata gaaaataaaa aaactttaga ggcaactttt
    11401 aatagtatta cagatataat aatgcttgta gatgaaaata acaatatagt agaatcgaac
    11461 aaagtactat atgactttat gggtgaaatg agttataaga tagcagattt gattagcatg
    11521 ataaaaggtg ttattaaaaa taccttctct gaaaatacta ataaaactag tgaaatagaa
    11581 atatacaata aaattttaaa gataaacact tttcctgtag agtataaaaa aaataatact
    11641 gagtatatag tagttcttat taaagatata actaatgaaa aaatagtaga ggcgaaatta
    11701 cttagagaaa acaaaatgat atctatagga caattagctt ctggggtagc acatgagata
    11761 agaaatcctt tgggtataat aagaaataat tgctatctat taaaagataa cgttacaatg
    11821 gaagaagtga atgattgtgt taaatcaata gaaagtaatg tagatagggc aagtaatata
    11881 ataaccaact tgttaaattt cgcaagaata tcagatgata atttagaaca tataaatata
    11941 agaaatttta ttgaaaatat agtgaaactt caatataaaa tgttacagtt aaagaatgtt
    12001 gaaataaaaa ttgactgtga acataattta gtatgctata taaatggaga atcgttaaaa
    12061 catgtattta taaatttaat ttctaattca atagatgcaa tacatcaaga tggaaaaatt
    12121 attatatact gttatgaaaa aaatcattgt ttatttatag attttaaaga taatggtgaa
    12181 ggaataaaag aagatgccct aaaggacata tttaatccct tctatacaac aaaaccaatt
    12241 ggtgagggaa ctggtttagg tttatatatt acttataatg agattaaaaa gaataatgga
    12301 gatataagtg ttgaaagtaa attgggggtt ggaacttgtt ttcatataaa aattccatta
    12361 aataaagagg tgactatata atgaaaatac tattagttga tgatgagcta gaatatggag
    12421 tggtaatgaa aaaaattcta caaaagaagg gctatcttgt agatatagca ctaagtggtg
    12481 aagaagctat aaatataatt aaaaaagata aaaattatga tttggtttta tctgatgtta
    12541 tgatgaaaaa tatggatgga gtacagctat tagatagaat taaatctatt aatagagata
    12601 tagaggttat attagttaca ggatatggaa gtattgaaaa tgcagttgat gctatgaaaa
    12661 gaggagcttt atcttatttt ataaaaagta atcccattga aaatttatta gaagaagttg
    12721 aaaaggtaaa ggcaagtaaa atatctgttt ctgtacaaaa aaataatttt gaatttactt
    12781 tggaatctaa aaatagagat tttaataata tcataaaaat agcaaaaaaa gctgcttgta
    12841 aagatgttaa tatattaatc ttgggggaat ctggtgtagg aaaagatata ttagctagat
    12901 atatacatag tataagtcca agaaagaatg agatatttgt acctgtaaat tgttgttctt
    12961 tttcagaaaa tctattggag tctgagttgt ttggtcatga aaaaggttct tttacaggag
    13021 cagtagatag tagaaaaggt agatttgaac tttcgaataa aggaactcta tttttggatg
    13081 aaataggaga tatacctcta aatgttcagg taaaactttt gaggacactt gaggacaaat
    13141 ctatagagag gataggaagt aataaatcta ttaaagttga ctttaggtta atttgtgcta
    13201 tgaataaaga accaaaagtg gaaatatcaa atggaaatat aagagaagat tttttttata
    13261 gaataagtac aataacaata actataccac cactaagaaa gagaagagaa gacttaacta
    13321 ctcttataga atttttctta aataagtacc aaattgaaca tgataaaaag atacactcga
    13381 ttgataagga agtaaaggat tttttattaa attacaatta tccaggaaat ataagagaat
    13441 taaaaaatat aataaataga cttgtagttt taagtgaaga aggcaattta tcaaaagaca
    13501 atttaaattt aatatcaaat aatgtataca tagatgataa aataagtatt agacctttaa
    13561 gagaaataag aaaagagttt gaatgtgagt atattgaaaa agttttatct ttgtgtggaa
    13621 ataatatttc aaatacagct aaaaaattgg agattagtag aagacagtta accaataaaa
    13681 tatcagagta taatattaag tgagaaaaaa acttctcatt aagtgagaaa aaaatttctc
    13741 aaactgtatt aaaagataaa gagaaaaaat ctgtttatct ttttttatta taattttaaa
    13801 aattaatatt tacaataatt attggtaaat tcaatactgg tgtaaattaa aaatgaaaat
    13861 ttctatgaca ttaata
//
LOCUS       DAELSY010000105        13857 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.105, whole genome shotgun sequence.
ACCESSION   DAELSY010000105 DAELSY010000000
VERSION     DAELSY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13857
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.105"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..394
                     /locus_tag="KPG09_002687"
     CDS             <1..394
                     /locus_tag="KPG09_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967283.1"
                     /translation="INAGFALYERSAQDLLTKLMTHENKISQNQKILLDEKFTLDDSI
                     KNLSSIIENFSNDLQVKLDMMFENSLDIQDKLDVMFNNSFMNDEVPLDSEELFKDDVN
                     NVFNPFSEEIYEIEDKEIKVIGEDELNE"
     gene            387..1019
                     /locus_tag="KPG09_002688"
     CDS             387..1019
                     /locus_tag="KPG09_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG0967284.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSKIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            1348..2505
                     /locus_tag="KPG09_002689"
     CDS             1348..2505
                     /locus_tag="KPG09_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0967285.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     FNSEEIVYFTPKGKSYHSIKNCSGLSRSKKILSGTIEESKKNDPCDICYGK"
     gene            complement(2653..3288)
                     /gene="lexA"
                     /locus_tag="KPG09_002690"
     CDS             complement(2653..3288)
                     /gene="lexA"
                     /locus_tag="KPG09_002690"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBG0967286.1"
                     /translation="MYLDLTEKQVLILEFIKSQITLKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(3392..4519)
                     /locus_tag="KPG09_002691"
     CDS             complement(3392..4519)
                     /locus_tag="KPG09_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0967287.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(4841..5686)
                     /locus_tag="KPG09_002692"
     CDS             complement(4841..5686)
                     /locus_tag="KPG09_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967288.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(5716..6087)
                     /locus_tag="KPG09_002693"
     CDS             complement(5716..6087)
                     /locus_tag="KPG09_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG0967289.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIEDACKILSCNGYEVE"
     gene            complement(6240..6500)
                     /locus_tag="KPG09_002694"
     CDS             complement(6240..6500)
                     /locus_tag="KPG09_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBG0967290.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(6689..7633)
                     /locus_tag="KPG09_002695"
     CDS             complement(6689..7633)
                     /locus_tag="KPG09_002695"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBG0967291.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(7654..8508)
                     /locus_tag="KPG09_002696"
     CDS             complement(7654..8508)
                     /locus_tag="KPG09_002696"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBG0967292.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKELLY
                     QILAMHKK"
     gene            complement(8523..9884)
                     /gene="accC"
                     /locus_tag="KPG09_002697"
     CDS             complement(8523..9884)
                     /gene="accC"
                     /locus_tag="KPG09_002697"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBG0967293.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(9900..10352)
                     /gene="accB"
                     /locus_tag="KPG09_002698"
     CDS             complement(9900..10352)
                     /gene="accB"
                     /locus_tag="KPG09_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBG0967294.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(10622..11068)
                     /locus_tag="KPG09_002699"
     CDS             complement(10622..11068)
                     /locus_tag="KPG09_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967295.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            11343..11621
                     /locus_tag="KPG09_002700"
     CDS             11343..11621
                     /locus_tag="KPG09_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967296.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(11750..12496)
                     /locus_tag="KPG09_002701"
     CDS             complement(11750..12496)
                     /locus_tag="KPG09_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967297.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLDFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(12625..13269)
                     /gene="trmB"
                     /locus_tag="KPG09_002702"
     CDS             complement(12625..13269)
                     /gene="trmB"
                     /locus_tag="KPG09_002702"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBG0967298.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(13407..13727)
                     /locus_tag="KPG09_002703"
     CDS             complement(13407..13727)
                     /locus_tag="KPG09_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBG0967299.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
BASE COUNT         4802 a         2151 c         1657 g         5247 t
ORIGIN      
        1 tataaatgca ggatttgctc tatatgaaag aagtgctcaa gatttattaa ctaaattaat
       61 gacccatgaa aataaaatct ctcaaaatca aaaaatcctt ttagatgaaa agtttacctt
      121 agatgactca ataaaaaatc tctcaagtat tattgaaaac ttttctaatg acctacaagt
      181 taaactggat atgatgtttg aaaattcttt agatattcaa gataagttag atgttatgtt
      241 taataactct tttatgaatg atgaagttcc tcttgatagt gaggaattat ttaaggatga
      301 tgttaataat gtatttaatc cttttagtga agaaatttat gagatagagg ataaagaaat
      361 aaaggtcata ggtgaggatg agttaaatga ataataaata tagaagaact gttaacagag
      421 aatttgaaaa gagtagcttt tggccaacat ttacagattt attatctact gtattgatgg
      481 ttgtgattct tatcttattt agctcagaaa gtatttcagg ttctgttgaa caagatttag
      541 ctaagaatgt caatgcttct gttgaagaaa cttttaaaaa aagcaaaata cctgtgaagg
      601 tagataaatc taatgggcaa gtaacttttg gtgaaaaaac catgtttgat gtagatagtg
      661 atgttttaaa accagaggct aaagaaatgc taaaaatgtt tgttccaaag tacatagaga
      721 ctatttataa agattatggt gactatatat ccaaaatagt tatagaaggt catacagatg
      781 atgtgggaag ttatatatac aacttagatt tatcgcaaag aagagcatat agcgttgcta
      841 aatttatagt gggagatgaa ataggtgact ataaatacaa agataaagtg actaagcata
      901 ttattgcaat aggaaggtca aaagctgaac tcatcaaaaa tggtgacaat agcgtgaata
      961 gagatgcatc tagaagagta gaactaaaat acgaaataaa tattaatcaa aataaataat
     1021 taaattttta taaaaaaaaa gaaggcagtt ggtgtgagct ttaagccttc tttaaggaaa
     1081 aagtttattt atttttgttg atacttaaca tattatagtt tttagaagca gtttgcaata
     1141 tattaaacgt aaatggtcgt ttaaaatgca taactggata ctactgatgt aaaatatttc
     1201 attttatata atataaaaat tatacagtgt caattttcta tattattgta ctataatcta
     1261 ttatttaaaa tattatgtaa aaaaatatca taaaaacgca tatcatgtta taattactag
     1321 agtattatga atatggaggc gtagtttgtg atagaaataa gtataaaaaa taaaaaaaat
     1381 tctaaaatta aaactttgat acttttattg atagtatcta tatcgattta tttaactggt
     1441 tgtactacat cagataatgg aaataatcaa tcaaataata atgattcttt gtctagtagt
     1501 atatcaagtg ataagaatgt aaaaattcat tttattgaca ctggaaatag tgatgcaata
     1561 ttgattcaag atggtaaaac ttttacattg atagatgggg gagataatga tgatgaggga
     1621 ttaatggtag attacctaaa caatcaaggt gtaaaagata ttaaatatct tatagctacc
     1681 cattctcatg cagaccattt aggtggactt gattctgttg tcaaaaactt taatatagaa
     1741 aatgtttttg tatcgaatgg ttcagcagaa acaaagagtt atagggactt tataaatgct
     1801 ttagcaaata agggtttatc cccaagtgta ccgcttgaaa ataataagtt ctatttagaa
     1861 gattcttatt ttgaagttct aaatacaaat gggggagata ctactaatga acaatctcta
     1921 gtccttgtat atactaatgg gaatgataaa gttttattta caggtgatgc tgaagaaggt
     1981 accgaaaaag aaatactacc taagcttgag aaagtagacc ttttaaaagt agctcatcat
     2041 ggttctcgta gtagttcatc tcaagagttt ttagataaag taaatcctga atatgcggtt
     2101 cttttagtag gcaagggtaa ttcatatggt catccacatc aggagacaat gaataaattg
     2161 aaaaaaatgg gagtaaaagt acatagaagt gatgagtgtt cagatataat atttgaatca
     2221 acaggtgatg gagtatttac aagttgtaaa gatggaagtt ataataaagg tgttagagaa
     2281 gatggaaact ctggaaaatc aaacagtaat acaagtaaaa atgattcttt taaaaataaa
     2341 caaaatacat tcaattcaga agaaatagtg tattttactc caaaaggtaa gagttatcac
     2401 tctataaaaa attgtagtgg gctttcaaga agtaaaaaaa tattatcagg cacaattgaa
     2461 gaaagtaaga aaaatgaccc atgtgatata tgttatggaa aataacaaaa aaacaaccaa
     2521 tttatctata aagcttctga actttaaatc atttaaaaaa taaaataaat gcataaaaaa
     2581 aataagctat aatcaataga taatacattt catctatacg aatagcttat ttttttaagt
     2641 attttttaat agctatttaa taactctaaa tacaccagtt acaataccta caatttcaac
     2701 ttctgagtca ttgagcataa ttggttccat aaaatcattt tctggctgaa gtcttattaa
     2761 atttccttct ttaaaaaacc tcttaactgt agcagattca ccatttataa gtgcaactac
     2821 tatctgagaa tttaaggccg tatttttttt gtctactaca acgtaatctt catctaaaat
     2881 tcctgcatta atcatacttt cgccttttac tcttaataca aaattgttac tgccctttac
     2941 taaactggca gggaatggaa tgtattcctc tatgttttgt tgagctaaaa tgggttctcc
     3001 agctgttatt tgacctacta aaggaagctc tattatttct tgattagctc cagaaacatc
     3061 atttacttta ctacgttcta aaacttcaat agctcttggt ttagtagggt cctttcttat
     3121 gtatcccagt ttttcaagtt tatttagatg agagtgcaca gttgaagtag atcttaaccc
     3181 tacagcagta catatttctc ttacagcagg tggataacct tttaatgtga tttgagactt
     3241 tataaattcc aatatcaaga cttgcttttc agttagatct aaatacataa catcactcat
     3301 ttccaaacat attttgtttt tatttccata cactttatat ttttaattat aacataatcc
     3361 atttatatct gcaaacattt gttcctgatt tttaatcgtc ctcaatccat tcaaaattct
     3421 tgactatatc acgttttaca tttttcttat gagctgcata aagctgagta gttgtgacat
     3481 tatcatggtc aagtaagttt tgaacatcat atatagaaaa tcctgtttgg attagagaag
     3541 ttgcagccgt tgctcttaat ttatggggac tatatccatt gtctctagta gtatcaagtg
     3601 gaatagatgt atattttttt accagtgttc ttatagcctt tgctgtaatt ctcttatttt
     3661 gaagagacaa aaataaagca tctttttctt catcatttaa tagttcatct cttgttcttt
     3721 cgttttgcaa ataatctttt attacatgtt cacaagtatg atttataggc attaaaactt
     3781 ctttcccacg ttttctatat attttaaatt cacctcttga aaaattaaaa gaggagatat
     3841 tgagttctct aagctcattt aatctaagcc cgtatgtaac aaataatgct agtatagctt
     3901 tgtcacgtaa tttagttttt ctccaatata ccttttcttt ctctgtaagc ccttcacctg
     3961 tttctactga ttcaagcatc ttagcaactt catctatctc aaggcgctta atagcatctg
     4021 gctgaggttt aggaagtttt attggattga atccatcagt tatattatta tctatctgag
     4081 agtttctgta taagaattta aatagagtag agatggatga tttttttctt gctaacgctc
     4141 tattgttatt ttcaaaaatc aatgtgtttt tttctgtatt tttgtaatat ctactgcaat
     4201 aatcacctaa gaataaattt atatctcttg attttattgt attaaaatca tcttctgtaa
     4261 tatcttttac acaatctgca tttgttaatt ctttggtttc aatcaaatat gaacaaaaaa
     4321 aattaatatc ttctaaataa gcaagtcgtg tagaaactgc aacagaacct tttaaatata
     4381 taaaataatc cttcataaat acaggaagtt tattttccaa aagtagacat ttttctatga
     4441 gttcattttc tttgataaca atattatcag gtttatctga tttattatgt acttttataa
     4501 ttttcttatc attattcaaa attaaacctc ctaaaactaa aatataaatc ttttatatta
     4561 ttttttgata tttataccaa gtgagtataa atatttaatt taatcatttt ttacattttt
     4621 aagagattag cagtgcttta gatgaaatta tcactaacta ttccctaaat ccacatttaa
     4681 agactagttg aatttaagta ctcttttttt atttctcaat atatttatag aaatatttta
     4741 aaatatattg agaaattttt tatgctctac taaaatcttt tttatataat atttttatat
     4801 atttctaacg aaaccaaata ttagtaaaat atgtatttat ttaactaaag tttttaaatg
     4861 ggctgactaa gcttaaaata taggatatta tgagagttac tattgaaaaa ataaacccta
     4921 ttgccataaa gaaacttata aatatgttat acttaactat taaactggat atattcaagt
     4981 tatcaaatcc aaataagtat gtaattgaac ttattacaaa aataactata actataaagc
     5041 tcagaccatt taagctattt tttaaatcag cactactaag tgacatatgt gtagatatag
     5101 aatacattaa aaacataaat ataaaatact tcaatgaata tacactaggt gttaacaaca
     5161 ttattcttat aaaattggaa atatcaccta aaataatgtg tataaaacca tttatattta
     5221 aattattaat catacttaca tataacttta aatctatatt ttgagtcact ttcatataac
     5281 tatctggcag taatatctta aaaattaaaa taatcattat tgttccacct ataataggtg
     5341 caattcctat aaagaaattt cctatacttt gataaagatt atctttttta tagctatgac
     5401 ttacatatcc tagtatacca tcatttttac tttctattgg tctgaaaaac ttaactgaat
     5461 ttattttatg catgaaaatc aagcacatta tcaaatgact caattcatga acaaacgttc
     5521 ctatagctcc agttacaata attccagttt ttccaaaaga tgaaaagata aatttattat
     5581 ttagattttc tgtaatgcta aaaatcaatc caaatacaat aaatattcct atgaaacaaa
     5641 aaatatttat tacacttatt tttaaaattt cgattatata aatcataagt taagtcctca
     5701 tgtaaaagta tttaactact ctacttcata tccattacag cttaaaattt tacaagcatc
     5761 ttctatatct ttttctttta ctaagatata gtctgtatca tacgtagaaa ttgcaaatat
     5821 gcttattttg ttgtctgcta acaaactact taacttagat aaaattccta caagtgaaaa
     5881 gtctaaagga cctagtatct taagtattct ccactccttt tctgacttaa catcagaagg
     5941 aatattatta tttaaacata caacacttaa ttcatcatca gttctagtta tagagtaaaa
     6001 cttctctgta tctatccatg ttggtatttt actgtcatta tttaatctac atactgcata
     6061 ctcttctacc aataatttta atttcataat gtatatttcc tccaatctta tttgtttact
     6121 taagattata cattaaaagt tacataaaat aaaacatata aagtaaaaaa ggttcactat
     6181 agtgtcctat ttctatagcc aacctttaaa acttaattaa attctaataa agagatatat
     6241 tatctaggtt caattataag tttaatggct gttttcttat caccttcgat ttctatatca
     6301 gtaaatgcgg gaatgcaaac taaatccatt ccactaggtg caacaaaacc tcttgctatt
     6361 gcaattgatt ttatcgcttg atttagagct ccagctccta tagcttgtat ttctgcaacg
     6421 cctcgttctc ttagtactcc agccaaggct ccagctacag aattaggatt agattttgat
     6481 gaaactttta gtacctccat ttcaacatct ccttgttaat ttgttaaagt atccattacc
     6541 taaagtacta aatcataatt gccatttact aattacaatc atcatttaaa gtatactttt
     6601 ataaaaatat acttctataa tcgttttaag aaaaccttta ttttttttaa ccattttatt
     6661 atgaaaaaac tacaaatttc tttatattct ataagcattt tccaatattt ctaaattttt
     6721 tatatctttg agctataatt tcctctttat ccatatcttt gtaatgattt aatctttcta
     6781 aaatataagc ttttatttct tttgaaacag cttctacatc tttttgtgct cctccaagag
     6841 gttcttttat aatcttatct attattttta aatcatataa atcattactt gtaagcttca
     6901 taacatcaca agcttcttta gctttagatg aatctttaaa tagaatacta gaaagccctt
     6961 ctggagaaat cacagaataa actgaatgtt ctagcataca aacatcattt cctactccga
     7021 tacctaaagc tcctccacta ccaccttcac caattacaaa agtgattact ggaacagaaa
     7081 gtttactcat ttcaagtaag tttcttgcaa tagcttctcc ttgtcctctt tcttctgctt
     7141 ctaaaccaca aaaagctcct gatgtatcaa taaacgttat tataggtcta ttaaattttt
     7201 cagcttgttt cataagtctt aatgcttttc tgtatccttc tggatgaggc attccaaaat
     7261 ttctttttat attttctttt gtatctcttc ctttttggtg tccaactata gtaacattaa
     7321 actttcctat ctgaccaatc cctccaacca tagaagggtc atctttaaaa agcctatctc
     7381 catgaagttc taaaaaatta gaacaaatat gttctatgta ttccaatgtc gtaggtctct
     7441 ttatatctct acttagtgtt accttttcat atgctgatag atgtgaaaaa gcatcttcct
     7501 taagcttttc caatttctca ttaagtgaat ttattttatc acttaaatct atattatttg
     7561 ccttagaaat agttattaat tgtttaatat cattttctaa gacttttatt ttttcactat
     7621 tattttcaat caaaacagtt cctcctttat gccctatttc ttgtgcatag caagaatttg
     7681 atataataat tccttcattt ttcttctatc tactatcatg tcaataaaac ctttttcaag
     7741 taaaaactca gaagtttgaa atccttcagg aagtttttga tttattgttt gctctattac
     7801 tcttggacct gcaaaaccta tcaacgtatt tggctcagaa attataatat caccaagcat
     7861 agcaaaacta gcagttactc ctccagtagt agggtcagtt agaactgata tatatggcaa
     7921 tgatttttct tcaagcttag aaagtgcttg agaagtcttt gccatctgca taagagatac
     7981 cataccttct tgcattctag ctcctcctga agcagaacaa ataattatag gtaaattatt
     8041 ttcagcagca tactctattg aataagttat tttttcccca acaatagaac ccatacttcc
     8101 catcataaaa tcaggattca ttacacaaat aactgtttta attccattta ttcttccata
     8161 acctgtgaca acagcttctg actcttttgt tttttcctta taagagtcta ctttttcttt
     8221 gtatttagga aaatttagtg gattttgaaa ttctacatca gaattaaact ccataaaagt
     8281 tcctttatca atcaataaat ttattctttt tcttgctctt aagccataat gtcttgaaca
     8341 ttttggacat acaaatgaat tttcttctat gtcctttcta aatacatgac tatcacaacc
     8401 tttgcaatag gtccaaaatt tatcatctac actattcttt ttaaagttgt catctaaaga
     8461 aatcgtaaca tacttagatt cttttgatga taaaaatttt tttatcataa tctatttact
     8521 ccttataatg tagcaaattc tttcttcata aagacatctt ctataaattt ggtatgatga
     8581 gtatcttctc taaactcctc acttgttaca agtgcctttt gaaattctac atttgtatta
     8641 ataccttcta caataatttc gtctagagct ctatatattc tacttataca ttcttctcta
     8701 gtttcagccc aacatattaa cttacctatc atagaatcat ataaaggtgg tatagtatat
     8761 cctgcatata caaaagtatc aaatctaaca ccaaaacctc caggtaaatt taatgattct
     8821 atcttacctg gtgatggagc aaaattcttt gatgaatctt ctgcatttat tctacattca
     8881 atagcatgac ctctaaaagt aatatcttct tgtttaaaag gaattttatc cccattagct
     8941 atagatattt gaagttttat aaggtcaaga tttgttacca tctctgtaac tggatgttca
     9001 acttgtattc ttgtattcat ctctataaaa taaaagtttt tatctttgtc aactataaac
     9061 tcaatagtac ctgcaccgat ataacctact ccttttacta tatctactgc aattttatga
     9121 agtttttgtc tttcttcatc tgaaaggtat ggacttaaag attcttctat tactttttga
     9181 tttcgccttt gcatagaaca atctctatca cctaaatgta ttgcatttga atgtttatct
     9241 ccaaatactt gtacttctat atgtctagga ttttctataa acttttccat atataaatca
     9301 ctattagaaa atgctgctag agcttctgaa cttgccatat caaaatttga aaaaagttct
     9361 tcttcttttc taacgattct catacctttt ccaccgcctc cacttgaggc ttttatcata
     9421 acaggatatc caatttctct tgcaacttct aaagcttctt ccttagtttt caaaacagat
     9481 tctgaaccag gaacaacagg gatattcaat tccatcatag tttctcttgc tctagattta
     9541 tctcccatca aaatcatttg ttctcttgtt ggacctataa aaataatatt attatcttca
     9601 cattgctttg caaattcagg attctctgat aagaatccaa agcctggatg aatagcatca
     9661 cactttaatt taagagcaag atagattata ttttctatat tattataact cttacttata
     9721 ttatttggtc ctatacaaat agattcatca gctatatatc tatgaaaaca atctttatct
     9781 atttcagaat agatagcaac agtttttata cctagttctt tacatgttct gatgattcta
     9841 actgctatat cgcctctatt tgcaactagt atttttttta tcaacttaaa tacctcctac
     9901 tacaatggtt ttatcttaaa taaaggttga ttatattcta ctagttcttc attactaact
     9961 aatacttcaa ttatttcacc tttaatttcg cttgttatct cattcataag cttcattgct
    10021 tctaatatac ataaagtgtc gccttcttca actacatcgc caactttaac aaagctatct
    10081 gcatctggac ttggagaatc atagaatgtt cccatcaaag gagctactac tactgataga
    10141 ttctcattag atgtgacagg tgtatcgttt acaactggct tttctaaaga aagatccact
    10201 actgattctt gttgtacact taaaacaggt gaacttgtag attgagcttt tttagatact
    10261 tctaatctta aatcgctact ttcaagtttt acatattcta agttagttga gtctatggcc
    10321 tttaataatt ccttaatttc attgatgttc attttgttac ccccttgtat tattacctgg
    10381 tgctaatatt atatcacaaa aaacaaattt ataaaagaaa aagtgctgac tagagaatca
    10441 acacatcaat atatctttta attttaaatt aacaaccagt aacttatatt tgttttaatt
    10501 ttccattatt aatataaatt ataagtataa aatgaataaa attcaatatt ttttgcaaaa
    10561 tacaagagaa tacaaattaa tacctcggga atttttaaga caaataccaa tattatgcaa
    10621 tttagacttc gctttctttc aaattagtat cattttcttt atttttacat aggatgtact
    10681 ttaatttagc tatcaaaaca ttactatata ggttaaaagt tattagaatt gcaggaatta
    10741 taaagtaaac tctagcacct ataaaaccaa aaactactgt aataagtatt aatcttttat
    10801 ttaaagtaaa aaaaggtata ttaaaaatac ttttgtcgga tgtttctgga gtataagaat
    10861 atataactcc aaaaatcaaa gtaagcatac aaagtggaag tagtgtataa cttgaaatta
    10921 ttcccagaaa tcctatactt tcaaaatatt tgctagcact atttgtataa acaaacatgt
    10981 atccatttaa atatgcgcat atacatgata taatgccaaa agttccccct aaggattgag
    11041 tgtaccaatt aaaaaattta gattccatat acctcacctc ctaaaactgt atatataatg
    11101 atatgcattt aaatagtaaa atatatcttt atatgtttct gaataaaatt ttgttaattt
    11161 cattaattta tgtttaagaa atttgaagta tagtgtagta tatattaatg tagatgaagt
    11221 tttatagtac ttatttcata gtgcttaatc aagttatctt attatggatt caaaagttgt
    11281 ttgtagggtt ttatattttg ttgagctgaa aaatttatta taaatgaagg gatgttttcg
    11341 ctttggtaga tacattagaa tttggtctta aaattttatt ttttatatta tcaataattt
    11401 ggatgggtaa aataatgatt ttaagaactg ataagcaaat agtaatcaat cctttgctta
    11461 ttggtatatc tgcagtcctt gtgatgttgc atacctcaca gagtaacatt gaattttttg
    11521 gattagatgt ccaatatata agaatagttt tgtatataat atattcactg ataattttaa
    11581 taggaatatg ggctactaac agaagaaatg gaatttttta gataatctat ttctataaaa
    11641 tttaaaggtt gctagtaaca aagcactaat attagcgttc tgttactaac aacctttatt
    11701 ttgattatac acttaaattg aaaatagaat gattgaataa gttgacatat tattttacat
    11761 tgacagtgat tagaacttct tgagatgtat ttaaatcatt tttgtcgctt agtataactt
    11821 ttaccacagc ttgacctgtt ttattacctt taattatcat atcttcacat gaaagtacac
    11881 tcttatcact tacagataca ataaagtcta aatcttttgg aagattatca gtaacttccc
    11941 agtatttata ataactatca attttcttcg taattatttt tctcaaatca atactttcac
    12001 cttttgctat atcaacatta tgatatttag agtccagttt atactgatta tcttttgtat
    12061 caataaaatt taaatcgcaa tttacatcca caccattatg agaaagagaa tttactacat
    12121 cgtcatcaat caaataatta ttggcaagat ttattgaatc catatctttt agacttaaag
    12181 cttccttagg aacctctttt atattattat tccaaaaaat aaaattgtct acattttcaa
    12241 gatatgatat tccttttaaa tttgaaatgc tctcaccagt tgcataaaat tctccatcta
    12301 aagcctttat attgcttata gtcatctcat ctgggtttat atatcttttt acaactgctc
    12361 ttaaatttgc atcaggaaaa atatctttaa gttgattttc gctcaaaacc ttatcatttg
    12421 aatttattgg acttaaagct gctgcagaaa taataaatgg tattaaatta gctaaattca
    12481 tagtaccctc ccacataaat tttttattat aataatttaa tttctatgag agggttctgt
    12541 ttccttgtta tatataccta ctttcgtaca ctttattaat ataaattaaa ataaatttct
    12601 actattaaac ttataaacat attactagtt acgtttttta gcttcacatc taaatattcg
    12661 cataccttgt gacataaatt tgtcttcata ctctgttgta acattatttt catactcact
    12721 atttcctaaa tcaagagaaa tattctttaa tagccaatta tttgcagcaa tttcatttaa
    12781 actaaactcg aataattttt cattatctgt tttaaagtga atttcgccat catcttccaa
    12841 aaccctattg tacatttcta aaaatccaga atgagtgagt cttcttttac tccatctatt
    12901 tttaggccat gggtcacaga aatttatata aattctcgat aattctttag cttcaaagta
    12961 atccaaaata ttgcttacat ctccccataa aaatagtata ttattcaaat tagaagcatc
    13021 tgctttttca acagctttta aaagaacttc ttccttaagt tccatggcaa tataatttat
    13081 gtcagggttc tgcttagcta aagttgttat aaacttacct tttccagtcc caaattcaac
    13141 atgtattgga ttatcatttc taaactttaa attccacttg ccttttaatt tgtctatatc
    13201 gtctcttaaa acatattttg tataacttaa aagcttttca tctgcacctt tttttcgtct
    13261 tcttctcaca ataattctcc ttacatataa aaaattagta aaatacattt ttattataca
    13321 ttatctcacc aaaaaataaa aagaactctc ttaaaataga aatttatttc tataaagaga
    13381 gttctaaact tactaaaaac ttaatcttag tggcaccctg aacaaccaga gcatcctcct
    13441 ccaccttgat ctttcatatt ttcaggaata actctaaatc caaatcctgt atcttctatt
    13501 attatatctc catactgact atattcatca gagctcatta caaattgtaa ttctccaact
    13561 tcataagtta agtcatcttc ttttttctca tctaaagcta atccaaaaga aggacctcca
    13621 caaccaacac ctgcaaagta tactcttata ttattaggtt tatcttgatt gtcttttaag
    13681 atacttttta aagtatcaat agctgtttct ggtaaagtaa ttttcatcaa atcattccct
    13741 ttcaaatatt ttattaaaac aatatctatt atatacccat acaaggtaca taatcaatta
    13801 gtttttgcat attttactaa aacttctacc taatattacc taaggcttta aaatatt
//
LOCUS       DAELSY010000106        13834 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.106, whole genome shotgun sequence.
ACCESSION   DAELSY010000106 DAELSY010000000
VERSION     DAELSY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13834
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.106"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(46..888)
                     /locus_tag="KPG09_002704"
     CDS             complement(46..888)
                     /locus_tag="KPG09_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0967300.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEISTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(1135..1629)
                     /locus_tag="KPG09_002705"
     CDS             complement(1135..1629)
                     /locus_tag="KPG09_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG0967301.1"
                     /translation="MSTLVTLLGLLVCTKISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKLSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPRMPLSVSLALAAVISSTDLVAVKSITQGLNFPKNMMSILEGESLLNDDDRII
                     NIME"
     gene            complement(1781..2752)
                     /locus_tag="KPG09_002706"
     CDS             complement(1781..2752)
                     /locus_tag="KPG09_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBG0967302.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPDEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTRALMSAVPVQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(2753..3730)
                     /locus_tag="KPG09_002707"
     CDS             complement(2753..3730)
                     /locus_tag="KPG09_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967303.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMVIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPVLGKNKDKRLYSIPGQV
                     PNPIGIPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            3949..5508
                     /locus_tag="KPG09_002708"
     CDS             3949..5508
                     /locus_tag="KPG09_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0967304.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKRKEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            5614..6573
                     /locus_tag="KPG09_002709"
     CDS             5614..6573
                     /locus_tag="KPG09_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0967305.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            6576..7388
                     /locus_tag="KPG09_002710"
     CDS             6576..7388
                     /locus_tag="KPG09_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0967306.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVISLTIISKKFSSNT"
     gene            complement(7603..8505)
                     /locus_tag="KPG09_002711"
     CDS             complement(7603..8505)
                     /locus_tag="KPG09_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0967307.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            8860..10038
                     /locus_tag="KPG09_002712"
     CDS             8860..10038
                     /locus_tag="KPG09_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG0967308.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINSGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            10047..10703
                     /locus_tag="KPG09_002713"
     CDS             10047..10703
                     /locus_tag="KPG09_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBG0967309.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            10703..11368
                     /locus_tag="KPG09_002714"
     CDS             10703..11368
                     /locus_tag="KPG09_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBG0967310.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     ILGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            11473..12249
                     /locus_tag="KPG09_002715"
     CDS             11473..12249
                     /locus_tag="KPG09_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG0967311.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            12311..13645
                     /locus_tag="KPG09_002716"
     CDS             12311..13645
                     /locus_tag="KPG09_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG0967312.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVLLNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKVGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSKLEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
BASE COUNT         4870 a         2023 c         2012 g         4929 t
ORIGIN      
        1 acataatata ttaaacctat acattctgta ttaaactact ctttattata aattcagtac
       61 agatataact cttttgatat gtaatgttaa ataaagtagt tcatcttgac ttacatagta
      121 atcataattc ttttctacat gagtttttat tttatatgca caatcataat ccttttcata
      181 ctttacttta attatatcta taatttcatt atttgtatca ttgatttttt ctttttttac
      241 taatcgtttt gcaaagaatt ttaaatgagt caaaagccta tcataattta tatcatcttc
      301 tttaaattca actctataat aatatcttat aatatttagt atgtctttta caattttagt
      361 aattaacagt gattcctttg aacttccctt atagtttgaa tttactatat gcattgctat
      421 aaaaccagct tcatctactg gaaatttcac atcaaattct ttatttatat aatctacagc
      481 ccatttacca atctcatatt cttttttatg tattcttttt atctcatgta gtaaatcatt
      541 ctttatagtt atattttcat gatatctttt aattgcaaat gcaatatggt cagtaagtgc
      601 tacataaata tattcataaa gcttagtatt caaattctcc tctgcataag caattatttc
      661 agtactaatc tcaaaaaatt tctcatcaac ctggcttgct agtttgctaa ttttatctaa
      721 aaagctttca tcttgtatta caaaagttct ttcaatttta cttttatcta tttcttgacc
      781 tttctttttt tgaaacccaa tcccacatcc tgttagtatt aattcttttt ttatattttc
      841 atctaaaact aatacaacat tattattata aatttgttga ataatcataa tcaatatctc
      901 tcccaaaata caatttccct tattcataaa aaaataccta aattaagata caattcacct
      961 gtatacctta acctaggtat tgcctgctta ccagtaacaa tccagcttgt taattcttat
     1021 atattgtatt caatttatgg ttttattcta ttataaataa atatatatgt caagtaaatt
     1081 attgaaaact atttcctatt ttttagtatt atttttaaca tcaaaaatca aattttattc
     1141 cattatattt atgattctat catcatcatt taataaagat tcaccttcaa gtatactcat
     1201 catattttta ggaaaattta atccttgtgt gattgatttt actgctacta aatcagttga
     1261 agatattact gctgccaaag caagtgaaac agataatggc atcctaggaa taagaaaatg
     1321 tataaagatt ccacctgcaa acacggttat caaaactaaa ccaaatgcaa gaagcaaaat
     1381 tggttttcta agctctagta actcttttct tgatacattt tgtccttcac tgaataataa
     1441 tggagctatg atacatatta caaatatttc tggattaaat gaaaatgata atttaaacgg
     1501 caaaatagac aatataatcc ctagtacaat ttgatatatt gcaagaggaa tattactcca
     1561 ctttttgcta aatacagttg aaatctttgt acaaaccaat aatcccaaca atgttactaa
     1621 agttgacata actacctctc ttttcaataa taatattata aattaaactt atatatcaac
     1681 taacttaata ttaacaacat gaaatcccat cttaatcaaa aaaattttca gtttattcta
     1741 ttattcttat tatatacatc aaatttaatt tttgaaatat ctatactaga tggcatgcaa
     1801 caaaatgatt tcttgatatt tcttttaaaa ctggaatctc ttttttgcat atttccttac
     1861 aataagggca tcttgtatga aacttacatc cacttggtgg atttgttgga gatggtatgt
     1921 ctccttttaa tataattcta tttttcttaa ttgtagggtc ttgtactggt actgcgctca
     1981 taagtgctct tgtatatgga tgttgagaat gtaagtaaag ttctccttta gttgcaattt
     2041 ctattataga acctagatac ataacacaaa ctctatgtgc aatatgattt actacactca
     2101 aatcatgaga tataaataaa tatgagagtt tatatttatc ctgtaaatcc ataagtaaat
     2161 ttataatttg agattgaata gaaacatcta gtgcagaaac aggttcatca caaactataa
     2221 attctggatt taatgctaat gctcttgcta tacctatcct ctgtctttga cctccagaaa
     2281 actcatctgg ttttctattt aaatggtact tagaaagacc acaactttct acaacattta
     2341 atatattatc ttttatttca ttctttggca ctaaattatg ttctataact gcctctgata
     2401 ttatttgttc cacatttaat cttggattta atgaactata tgggtcttga aatatcattt
     2461 gcattttggg tcttaacttt ctaagttctt ccttctctaa cttatatata tctacaccat
     2521 taaattcaac tttaccttca gttttttcaa ttaatctaag gatggttctt cctgcagttg
     2581 attttccaca tcccgattct ccaactatag ccaaagtttc tccttttttt aaatcaaaag
     2641 aaattccatc tactgccttt acacatttaa tttccttatc aaataatcca gatttaactt
     2701 caaagtactt tttaagacct tctactttta ataaactctc ttcttttttc aattatatct
     2761 cctccttttt atataaccaa catgcaatac tatgaccact attcaattct attaaaggag
     2821 gtatctgagt tctacattta tcacatactt tttcacatct atcactaaaa taacatgaat
     2881 ctggtattcc aattggattt ggtacttgtc ctggaatcga atacaatctt ttatctttgt
     2941 tttttccaag aacaggcttt gattttagaa gtccaatagt ataaggatgt tttggatttt
     3001 taaataattc aacaacctct gccacttcta taattttacc agcatacatc actaatactt
     3061 tgtcacacat ttctgcaact actcccaaat catgtgtgat catcattata gatgtgttta
     3121 atttttcttt tatatttttc attatatcta aaatctgagc ttgaatagta acatctaaag
     3181 ctgttgttgg ttcatcagct ataagtaatt ttggattaca tgataaagcc atagctatca
     3241 ttattcgttg tctcatacca cctgataatt catgtggata tgaattatat atttcttcag
     3301 ctcttggtat ttctaccagt tttatcatat cgattacctt tttcttagct tcttcctttg
     3361 ataaatcttg atgtatcata ataccttcaa tgatttgact tccaacagtg aacgctgggt
     3421 ttaaagatgt cattggttct tgaaaaatca ttgatatctc attccctctt attaccataa
     3481 gttcatcttc attcatcttt aaaatgtctt tgcctcgaaa gattatttct ccaccttcta
     3541 tttttgatgt tggaagtaac ttcatcaaag acatggctgt tacagactta ccacatcctg
     3601 attctccaac tattccaata gtttcacctt catttatatt aaaacttact tcatttacac
     3661 tctttacaat ccctttatct gtatgaaaat aaacttttaa atctttaacc tcaattagtg
     3721 ctttactcat tattttctcc tctcagcaaa attacttaaa ataacataca ccttttgatt
     3781 gtattttata aaaatttaaa ttacaacatt ttcttacatt ttattatttt tttctattaa
     3841 ttataataat ataatagtta atttctatat atattgattt aataatgatt actattatac
     3901 aatgaaaaat atgtgttaaa tattatttta tggaggtttt ataagaatat gaaatttaaa
     3961 aaattggcat cattaatctt agtttcatca ttaatgctta catttacagc ctgtgcttcc
     4021 aatgataaag acaagtctag tggaagcaaa tctggtggaa ctgttgtaat acctatggca
     4081 agtgacccag atataatcaa tggagctttt gcaaacgtta aagaagactc tcttgcatca
     4141 aatatagttt atgctcctct ttatacttat gaaaaaggga atttagtaaa ttatttagca
     4201 gaaaaagtag attttaaaga tagtaaggaa ttaactataa aattaaaatc caatttaaaa
     4261 tggcatgatg gaaaaccaat aactgctgaa gatgtcttat ttacatttaa tacagtttta
     4321 gacgaaaaac aaaattcacc atcaagacaa tacttattgg tgggtgaaaa accagttaaa
     4381 gtagaaaaaa ttgatgattt aacagttaag attactttac caactgcaag tgaatctttc
     4441 ttatatggta tatctaaaat ttctccaata ccaaaacatg tatttgaagg agaatcaaat
     4501 atagctaagt cagaaaaaaa taataatcca gttggttctg gtgcattcaa gtttaaggaa
     4561 tggaaaaaag gtgaaagcat agtatttgaa aagaatgcag attattttgg tggagaacct
     4621 aaggcagatt ctatagcgct aaaaataatt cctaatgaag catctcaaga agcagcttta
     4681 aataatggcg aaatttcact tatgaagaca tctgctgaag gatatgaaaa ggcaaaatca
     4741 aactcaaact tacaaactta tacttatagt gaagaaagat taaactacat tgtatttaac
     4801 caaaatatat caaacatggc taacaaagaa gtaagacaag ccttatcata tgccttaaat
     4861 agaaatgaaa tgatagaatc agcatatgga aaagaaggtt ctgtacctgc taaatctata
     4921 ttagttccag aagcagactt ctatacagaa gaaggtgttg aaggatatga ccaagataca
     4981 aataaggcta aagacttatt ggataaaagt ggtgttaaaa ttgataaatt aaaaataggt
     5041 tataacacag gtagatttgg gcataaaaac tatgccttag tagctcaaca agagttgaaa
     5101 aaaattggta tagaagctga aatagttcct tatgaaagta aggcattctt taatatacta
     5161 tttagtaata gcactgaatg tgatatgtat gtaaatggtt atgcttgggg attagaacca
     5221 aacccatata gaggtatgtt tgaaactggt caatattgca accaaactaa atattctaat
     5281 gctgaaatag atgctttatg ggaaaaagga tttactgagt taaacaagga aaaacgtaaa
     5341 gaaatataca aacaaataca acaggatata tctaaggatg cacctattta cactatagat
     5401 tatgaacaaa atttaatggc agctcaaaaa aatctaaaag gaataaaaga tgctaaacca
     5461 tctcctgcaa tattatttga agattggtct aaactgtatg tagaataatt gatgtaaaat
     5521 tttatattat taattagaaa aggagatgtc taaaaataat attgttttat tattttaaaa
     5581 cgagacatct cttttaatta ggagaattta cttatgttaa aattaatatt gaatagagta
     5641 aaagttttaa tacctatgtt aatcttaata tctatattat catttggact tttagaatta
     5701 gctcctggtg acccagcaga tgcatatata aatcctttaa tgtcttcaca ggatattgaa
     5761 aatataaggg ttaatatggg gcttgataaa ccagtatata taagatattt aaactggttg
     5821 aaaaacactt taagtggaaa cttaggtatt tcttatataa accatatgcc cgttacagaa
     5881 caaattatgg aaaaaatggg aaatacattt attcttatgg gtacttcttt gatattttca
     5941 atattggtag ctatacctct tggaatattt ttagctgtaa ataaaaattc aataactagt
     6001 aaagtatcat ctgtttttaa ttatataggt gtttctatcc cttctttttg gataggtatg
     6061 atactcattt caattttttc agttaaactt aacattttcc catcaggagg tatgcatact
     6121 attggaaatg atagtattgg agatttagta aaacatttag ttttgcctgt aataacttta
     6181 ggtctatata atactgctat ttttacaaat tatgtagaag ctagtgtaaa tgaacaatta
     6241 aaaaaacaat atgttattac agcaagagca aaaggattat cagaaaaagt catactattt
     6301 aaacatgtct tgaaaaattc actgacatca ttggttacaa tcctaggcat gagtatacaa
     6361 aaattagtca ctggtgcatt tgttacagaa gtagttttct cttggccagg tatgggaaga
     6421 ttaatgattg attcaatttt ttcaagagat tatactgtaa ttatggcaat aactatgtta
     6481 tcagcactat ttttaatttt aggtaattta gttgctgata tactatactt attaattgac
     6541 cctaaaataa aatctagcaa aggaggattc tagttgtgtt tacaagtatc ttaaaaaaat
     6601 taaaaaaaga taaatccttt gcttttgcat gtattactct tagcataata atcatattaa
     6661 ttatagcttc ttttatatta aatatagact ctaatgcaat tgatactact tctaaacttc
     6721 aagaacctag cttaaagcat atctttggta cagacgaact tggtagagat tacttctcta
     6781 gagctcttta tggtggaagg atttcactta gtgttggtat tttatcaatg cttatctctg
     6841 tagtatttgg tactgctgta ggtgttatta gtggatatat tggaggtaag ctagaccaat
     6901 tcttgatgag attagtagat atgcttatga gtataccaag tttcttagtt ttgattgtat
     6961 taaatactta tctaacacct aaagtttcca ctattatact aattattgga ttcttaagtt
     7021 ggatggaagt ttcaagaatt gtacgtggcg aaacattaaa aataaaggaa aatgaatact
     7081 gtttagctgc taaagctact ggtatcaaga ctagaaatat actattaaga catatagttt
     7141 taaacttaaa agagactatt gtagtcgctg gttcgttaaa tattgctaat gctatcttaa
     7201 ctgaatccgc tcttagtttt ttaggtcttg gaatccaatt acctcttgca tcatggggta
     7261 atatgcttca agatgctcaa agacatattt ttgacaagtt atatttagct gtatttccag
     7321 gattacttat atttttaaca gtcataagtt tgactataat aagcaaaaaa ttttctagta
     7381 atacttaaca aattttatat agaaaataaa aacagtgatt atctaaagat atacactgtt
     7441 tttatttttc gtttaaaaaa tacatatttt gtatattata actttaaata ctactctacc
     7501 atgttatgta aagcctatta ttgctctcaa acatatagat gaaaaactaa tagtttattg
     7561 taataaaaat tgatttattt atttgttatt ttaataacat ttttaagagt tctttttact
     7621 aaagaaatct ataataaatt taaatacatg ttggtctaca tggctataac gttctttttt
     7681 aggtctacat agtataactt tccatgtaat aggatgatta aaaggctttt ctataatttt
     7741 gctattttca aaaaaatcat gaactggtga tggtaaaatt gttataaaat cagaatttgt
     7801 ggttgatagc aacaaaaaat cccatgaacc agacattata tatcttttta acttaacatt
     7861 ttcatcagta aatttttgca ttagcttatg atgtatcata aaagtaggat taaatattgc
     7921 aagtaattgc tcattcaaat cgttccaatc tatcttatcc ttcttagcaa gtggatgatt
     7981 tatatttaaa aatgctgtaa gttcatcttc ttgaagcaca tgttcagtta ctacactttt
     8041 atcaatttta tgtggatgta acagtactgc ataatctagt tcctgcaaga taagcattct
     8101 atttaagtca tatgcaccct tctctactat atcaaattct atatctggat tatttgcaac
     8161 taactgtgca actacatctg aaaatgctat tccaagtata agtggaggta ttcctattct
     8221 aacttttcct ttaaattgaa ctgaatcttc tcttaaatct tccatcatat ttttgtattc
     8281 atttataata tgttctgcat ttacataaaa tctttctcct gctggtgtta acccatttaa
     8341 cctaccttta tacctttcaa ataaatatac atcttcactt tcttcaaaat tttttataat
     8401 ttgacttaat gctggttgag atatgtgaag tgtcttagat gctattgata aattaaaacc
     8461 agatttcact atttcaacaa aatatttcaa ctgttttata tccattttaa acccccttcc
     8521 ccaaaagtat aatttagatt atattttttc ttctaatgat attttatagg aaaatcaaca
     8581 taatttcatt aatattttaa caaaatattt ttctaaatct actataaatt tttatcctat
     8641 aaatttttct tatacactat ataaaaaatt agacttagac aaaatgcttt tttcgccgta
     8701 atctataatt agaaacagga aaacctaaag cattaggaat aaaagtaatt gaaatactta
     8761 aaaacaagct tgtattaaaa aagtacttta ttatagtgtt ccctaatttc tttattcaga
     8821 aaatttttaa ttatcattca agaaaggtaa ctattagata tgaaagatgt agtaatcgta
     8881 tcagcagtca gaacacctat aggaagtttt ggaggtgtat ttaaaaatac ttcagctgtt
     8941 caacttggaa ctatcgctgt taaagaagcg atatcacgtg taggacttaa tttatctgaa
     9001 atagatgaag taataattgg aaatgttctt caaacagggc ttggacagaa tgttgctagg
     9061 caaattgcta taaattcagg tataccaaat tctgttccaa gctacacagt aaataaatta
     9121 tgtggttcag ggcttaaaag tgttcagcta gctgcccaat caatcacatc tggtgaaaat
     9181 gatgtggtaa ttgcaggtgg tactgaaaat atgagccaag ctccttatat cgttccaact
     9241 gctcgctttg gtagtaaaat gggtgacata accatggtag atagtatgct aacagatggc
     9301 cttattgatg ctttcaatca gtatcatatg ggaataactg cagaaaatat tgcaactaaa
     9361 tttgaattta ctagagaaat gcaagataaa ttggcattag aaagtcaaaa taaggctgag
     9421 aatgctataa aaaacaaccg ctttaaagaa gaaatcgttc cagtagatgt tttaatacgt
     9481 cgaggaaaaa tagaaactat tgataaagat gaatatccta aattaggcat gacatttgaa
     9541 ggtttagcta aacttaaacc tgcattcaaa aaagatggta ctgtaacagc tggaaatgcc
     9601 tcaggtatta atgatggtgc ggctatgtta atactaatga gccaacaaaa agcagacgaa
     9661 ctaggtatta gaccacttgc taaaataaaa tcctatgctt cagctggtgt tgaacctgaa
     9721 gtcatgggta ctggtccaat ccctgcaact agaaaagcac tgaaaaaagc tggacttagc
     9781 atcaatgata tagacttaat agaagcaaac gaagcgtttg ctgcccaagc attagctgtt
     9841 aaaaatgaac ttcaaataga ttcatcaaag ttaaatgtta atggtggtgc aatagcatta
     9901 ggacatccaa taggagcaag tggagctaga atacttgtta ccttaattta tgaaatgcaa
     9961 aaacgtaaag ttgaaactgg acttgcaaca ctttgtattg gtggtggtca aggaatatca
    10021 atggtggttt cacgatagga ggaaatatga ataaaatagt tagcattgat gaagctctat
    10081 ctcatgtaaa agatggagat acaataatgg ttggtggatt catggccaat ggttcaccag
    10141 aaaatttaat tgattattta tgtagtaaaa atataaaaga tttgacacta atttgcaatg
    10201 atactggctt tattgacaaa ggagttggca aaatggttgt aaataagcaa tttaaaaaga
    10261 taattgcttc tcatgtagga ttaaataaag aaactggaag acaaatgaac aacaatgaaa
    10321 cagatgttga gttagttcct cagggaactc ttgctgaaca aatacgtgca gctggttatg
    10381 gtctaggagg tatactcaca ccgactggta ttggtactct tgttgaggag ggaaagcaaa
    10441 aaattgttgt cgatagcaaa gaataccttc ttgaaaaacc aatttatgca gatgttgctc
    10501 ttctatttgc atcaaaggtc gataaagcag gtaatctagt ttataaaggc tctatgaaca
    10561 attttaataa tttaatggca tctgctgcca aaattacaat agttgaagca gatgaaatcg
    10621 ttgaaattgg tgacattgac ccaaatgaag ttaatacacc aggtattttt gtgaattaca
    10681 tcgttgaagg aggtaattta taatggataa attagaaatg caagaatata ttgcaaatag
    10741 agtatctaaa gaattaaaag acggagccgt tgtaaacctt ggaataggtc taccaaccaa
    10801 agtagcaaat tatattccgg ataatgtaaa cgttatctta caatctgaaa atggcttttt
    10861 aggtttaggt tcagccgaag atggaaattc aagtgatgaa actattgtaa atgcaggtgg
    10921 tcaaccagtt actatacttc ctggaggatg tttctttgat agtgctactt cttttggaat
    10981 aatcagagga ggtcatgttg atataactat tcttggcgca cttcaggttg acaaatatgg
    11041 caatatagca aactatatga ttccaggtaa gatggttcca ggtatgggtg gagctatgga
    11101 cctagtaact ggagctaaaa aagttattgt agcaatggaa catacttcaa aaggttctgc
    11161 aaaaatatta aataattgta ctcttccact tactgcaaca aatgctgtag atttgatagt
    11221 tactgaaatg ggagttatgg aagtaacatc agatggaatt ttacttaaag aaataaatcc
    11281 agctttcaca ttagatgatg ttatttctgc tactgaagct cctttaatat tgtcagattc
    11341 attaaatgga aaaatatctg ttgtttaaaa taattaaaac tagaaaataa aaatctctta
    11401 gacgtattac atataaattt gtattgctta aaatattaat ataaaaataa aataaaattg
    11461 gaggaaatga aaatggttaa agataaagtt gtattcgtaa caggagctgc aagtggaata
    11521 ggtaaacaaa tagctgaatc gttcttaaaa aatggtgcta aagtaatgtt ctctgatata
    11581 aatcaagaag ctttaaataa taccataaca gagttacaaa aaaaaggtta tgattgcatg
    11641 agtgtaaagt gtgatgtaac taaggaagaa gaaattaacc acgctataga taaaactgtt
    11701 gaaaagtatg gcagattaga tatattaata aacaatgctg gacttcaaca tgtttctatg
    11761 atagaagatt tcccaactga taaatttgaa tttatgataa aaataatgct gacagcacct
    11821 tttatagcta ctaaaaaagc atttcctata atgaaaaaac aaggctttgg tcgtataata
    11881 aatatggcct caatcaatgg tgtaatagga tttgctggaa aggctgctta taactcagca
    11941 aaacatggac ttattggact aacaaaagtt gctgctcttg aagctgccga ttctggtata
    12001 acagttaatg ctttatgccc tggatatgta gatactccac ttgtcagagg tcagtttaat
    12061 gacctttcaa aaactcgtaa tataccatta gaaaatgtct tagaggaagt tttgtaccca
    12121 cttgtaccac aaaaacgcct attagatgtt caagaaattg ccgactatgc tatgttccta
    12181 gcaagtgata atgctaaggg tattacagga caagcttgct tactagatgg aggatatact
    12241 gcacaatagt tattatattt agggcttgaa gaaagtattc ttcaggtcct tctactaagg
    12301 agggatattt atgagtatta tcgcaccaat tggtataatt attggtatca tagctattat
    12361 atatttttca gttaaagaaa ttcatataac tatagctgct ccaatagcaa ctttaatagt
    12421 tgttctctta aataaaatgg atgttgtaac atcaatgcta ggtgctgaca aaaacaatta
    12481 tatgggtgca cttggaaact atataatgag ttattttgca atattcttat taggctcaat
    12541 acttgctaaa tttatggaag aaagtggagc cactgtatca atagctgatt ttattttaaa
    12601 taaagttggc tctaatcatc cttatagagt tttagttgct atatttatag taagttttat
    12661 attaacgtat ggtggtataa gcttatttgt agttatgttt gcagtcattc cacttgcaag
    12721 aactctattt aaaaaacttg atatttcttg gaaccttatt cagataccac tttggcttgg
    12781 tattgcaaca attactatga ctataattcc tggaacacca gccattcaaa atgttatacc
    12841 tattcaatat ctaaatacgt ctcttactgc agctactata ccaagtattg taggtagtat
    12901 aggttgtgct acgtttggac ttgtctatat gaaatatgct ctaaacaaaa gtattagaaa
    12961 aggtgaaact tacgcaactt acacttcaaa attagaagaa gaatctttag atagagaatt
    13021 accaggattt tttgcaagta tttttcctct attagcactc gtaattatag ctataactgg
    13081 aagtactttt ggttcagaat ttatgaaaaa aaatgtaatt tacatagctt taatagttgc
    13141 aattctttta tctgcttttt tattccataa atttctatca tctaagatac agacattgag
    13201 cataggtgct agtggttcta taggtcctat atttgctaca tcttctgcag ttgcatttgg
    13261 agcagtaata atggctgcac ctggttttga tgtatttagc aaacttataa tgtcaatacc
    13321 aggtagtcca cttataagtc ttactgtttt gacttcaaca atgtcagcaa taacagggtc
    13381 ctcttctggt gcattaggaa tagtaatgcc aaactttgca gattattatt taagtactgg
    13441 tctaaatcca gaattaattc accgtgttgc tacaatagcc tcaaatatac taactatagt
    13501 tccacaaagt ggtgtttttc ttacattctt aagcttatct aagttaacac ataaaactgg
    13561 atttaaagaa actttcattg tagttgcagt tggttcacta atagcagaaa ttattgtaat
    13621 tacattagga ttactaatgt attaatataa aacaaattaa aattcataat atgaaaggtg
    13681 gtgtctcaaa gtgaactttt tacttaatga gacactcttt ttgtatgaaa tcaaatatat
    13741 tttaatcatt tcaacaataa aaaagaacct ttctctattt tgagacaaac cctttttatt
    13801 ccttaaagat ttaaacaatt ataatttgtt acta
//
LOCUS       DAELSY010000107        13616 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.107, whole genome shotgun sequence.
ACCESSION   DAELSY010000107 DAELSY010000000
VERSION     DAELSY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13616
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.107"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1045
                     /locus_tag="KPG09_002717"
     CDS             <1..1045
                     /locus_tag="KPG09_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBG0967313.1"
                     /translation="INQLVYQLRDFRSLIGESLFYPFLFTITSLAIYSMISYKRNKQI
                     VDFIRKTNRKNQKLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKIN
                     NIVHQLRNITIEERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMY
                     YVDIAYEKSKNLNVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEF
                     MVSRINFNEEKLIVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKMEDMAVVKVI
                     NYGEPIPVLDLPNIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQ
                     TVFEVQLPIIQSA"
     gene            1327..1782
                     /locus_tag="KPG09_002718"
     CDS             1327..1782
                     /locus_tag="KPG09_002718"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBG0967314.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            1772..2497
                     /locus_tag="KPG09_002719"
     CDS             1772..2497
                     /locus_tag="KPG09_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBG0967315.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLNQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            2469..4850
                     /locus_tag="KPG09_002720"
     CDS             2469..4850
                     /locus_tag="KPG09_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBG0967316.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDNAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSCGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(5055..6284)
                     /locus_tag="KPG09_002721"
     CDS             complement(5055..6284)
                     /locus_tag="KPG09_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG0967317.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            6884..7798
                     /locus_tag="KPG09_002722"
     CDS             6884..7798
                     /locus_tag="KPG09_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967318.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGVKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            7822..8457
                     /locus_tag="KPG09_002723"
     CDS             7822..8457
                     /locus_tag="KPG09_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBG0967319.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTAQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            8833..9549
                     /gene="pyrF"
                     /locus_tag="KPG09_002724"
     CDS             8833..9549
                     /gene="pyrF"
                     /locus_tag="KPG09_002724"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBG0967320.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVRAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGADFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            9575..10618
                     /gene="carA"
                     /locus_tag="KPG09_002725"
     CDS             9575..10618
                     /gene="carA"
                     /locus_tag="KPG09_002725"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG0967321.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGEILTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIAPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            10671..>13616
                     /gene="carB"
                     /locus_tag="KPG09_002726"
     CDS             10671..>13616
                     /gene="carB"
                     /locus_tag="KPG09_002726"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBG0967322.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENG"
BASE COUNT         5490 a         1495 c         2535 g         4096 t
ORIGIN      
        1 aattaatcaa cttgtatatc agttaagaga tttcagaagt cttataggag agagtttatt
       61 ttatccattt ttatttacaa tcacatcatt agcaatttat tccatgatat cgtacaagag
      121 aaataagcaa attgtagatt ttataagaaa gactaataga aaaaatcaaa aattagaaat
      181 cattaaaagt gctgtaaatt taatggatga aggtaactta gaaaaaagta tagatattga
      241 gtatgattta gatatattaa aggataaaga aaaggatgat attgatgaac tagcacataa
      301 aatcaataat atagtccatc aacttagaaa tataactatt gaagagagac aagctcagca
      361 gacaaaaaca gatttaataa caaatgtgtc acatgactta agaacacctt tgacatccat
      421 aatgggttat ttaggagtga tagaagaagg taagtataaa gatgaagttc agatgatgta
      481 ttatgttgat attgcatatg aaaaatcgaa aaatttaaat gttcttataa atgatttgtt
      541 cgaattgact aaaatgcaaa ataatacaat aaaactaaac aaaatagaaa taaatttagt
      601 agagttatta agtcaagtag tatctcaatt tgaaatttat tttaaacaag aatttatggt
      661 tagtagaatt aattttaatg aagaaaaatt aattgtgaaa gctgatccaa ataaattggt
      721 aagagcattt gaaaatctta taactaatgc tgtgaaatat ggaaaagatg ggcattatgt
      781 agatatagtc acagagaaaa tggaagatat ggcggttgta aaagtgataa attatggaga
      841 accaattcca gtgttagatt taccaaatat atttgataga ttttatagag ttgaaaaatc
      901 gagaaataga aatgttggag gttctggatt gggtcttgcg attacaaaaa atattgtaaa
      961 tttacatgat ggagaaatta ctgtttcaag cgataaatat caaactgtgt ttgaggtaca
     1021 actgccaata atacaatcag cttagttata aaataaaagt acgattatgt gatataaata
     1081 ttataacaat aaagtgttta caaaaataaa aaagtgatat ataatttaag tcagtttaat
     1141 aagatactta tcaagaatta ggggagagag ttagctcagt gatcctatac caacctttta
     1201 tcaggtggtc ctgctaacaa cgatgagatg acttgaaata atatactctt cttatcaata
     1261 atagatagga agagtttttt ttagaattaa tgtaacaaac tttaaagtat aatggaggat
     1321 taaataatgg aaaaagtaga aagttttaag ctagaccata caaaagtaaa agctcctttt
     1381 gtaagaaagt gtagtgtact tgatggagta aagggagata aagtaactaa atttgacttg
     1441 agatttttac agccaaatgt agaaagtttt ggaactgctg ctatgcacgg tttagagcat
     1501 cttttagcta cttatttaag agatacatta gatggtgtaa tagacttatc tccaatggga
     1561 tgtagaacag gattttattt aatactatgg ggagatgtag atgccaaaac agttaagatt
     1621 ggtttagaag aagcattaaa aaaagttctt gaatcagata aaatgccagc agctacagcc
     1681 atagaatgtg gaaactacag agacttatct ttatttgggg caaaggaata tgcaaaagat
     1741 gttttagata aaggattttc tttaaatata tatggagaat aatttaattg gaagagaaga
     1801 tataagcata aatcaaagtg aagttttaag atatttacaa tacagaggtc aagagataga
     1861 taaagacctt aaatatacta ttgatgaatg tgtagctata actaaaaata aaataaatcc
     1921 tagatattta tctaggattt atcctattag aataaataaa gataatgttg tagaattaga
     1981 gggaacaaat ttaacattag agagtaaaga tgtatatgaa cttttaaaag aatgtaatga
     2041 gtgtgtaatt atggcagcta ctatagggat agatatagaa aaagaaataa gacgacactc
     2101 ttattcaaat ttaacaaaag gactaataat tgattcatgt ggaacaacag caatagaaga
     2161 agtgtgtgac attgttcaaa ataatttaga aaaggttttt ttaaaccaag ataaatttat
     2221 aactatgcga tatagcccag gatatggaga tttaccaata gaaaaaaata tagatatatt
     2281 aaacgttttg aatgctcaaa aacaaatagg tctaacaatt acttctaatg gaattatgat
     2341 accgagaaag tcagtagtgg ctattatagg tatttctaat acaagaataa ataaatctaa
     2401 gaaagaaaag tcgtgtgaaa attgtaaaaa ctatagaaac tgtacatata gaaaaggagc
     2461 gaactcttgt ggaaataaga caatatctga aaaataatat attaatattt gatggtgcaa
     2521 tgggaacaat gcttcaacaa aaagggttaa agttaggtga aaatccagaa gtatttggtc
     2581 ttcaaaatcc agataaactt atagaaatac atacagcata cttagaggca ggttcgaatg
     2641 taattcttac aaatactttt ggatgtaatg aactaaaatt agatagtaag tatactgtag
     2701 aagaggtgat tgacaatgct gttttggtgg ctagaaaagc tatagaaaat gtagataata
     2761 ctaaaccaag atatgtagct ttagatattg gtcctatagg agaaatgcta gagcctatgg
     2821 gtactttaag ttttgataat gcatatgaga tatttaaaag acaagtacta caaggcgtta
     2881 aatctggagt tgatgtaata gtaatagaga ctatgatgga tttatatgaa gctaaagtag
     2941 ctgtattagc agcaaaagaa aactctgatt tacctatttt ctgtactatg acatttgatg
     3001 aaggtggaag aagttttact ggatgtatgc cagaatgtat ggtggctact atagaaggtc
     3061 taggtgtaga tgcaatagga gttaattgtt cattaggacc taaacaactt ttaccaatag
     3121 ttgaaaaaat agcatcaaga gctacagtcc ctgttatggt tcaagcaaat gcaggtcttc
     3181 caaatatagt tgatggagag gctatatatg atgttgatgc aaaagaattt tttgaagggg
     3241 ttaaaaagtt cgttgaagtt ggtgctacta taataggtgg atgttgtggt acgaatccaa
     3301 gttttataaa agaaatatct gagaatataa attctgttac aaagggatgt atcgagaaaa
     3361 ttgataaatg tgtagtctgt tcaccatcaa aatttgtgga agtacaatcg cctacagttg
     3421 ttggagaaag attgaatccc acaggtagaa aatctctaca agaagcatta aaaaatgaaa
     3481 atgttgatta tgctataaat ttaggcttag aacaagtaaa tgctggagca caaatacttg
     3541 gagtaaatgt tggtcttcca gagattgatg aaaaaaaact aatgccaaaa ttaataagag
     3601 agattcaagc agtagtagat acacctttac aagttgattc ttccaatgta gaggccttag
     3661 agcaaggact tagatattat aatggtagaa caatagtaaa ctctgtaaat ggcaaagagg
     3721 agtctttaga aagtatatta ccaatagtta aaaagtatgg ttcatgtgtg gttggactta
     3781 cacttgatga aaaggggata cctaaaaagg ctgaagaaag atttgaaata gcaaaaagga
     3841 tagtcaatag agcagtttct tgtgggataa aaccaaaaga tatatttata gactgtttat
     3901 cattaactgt ttcagcacaa caagaagaag caatagaaac tataaaagct ataacaatgg
     3961 taaagacact aggagtaaaa actatattag gtgtttcaaa tatttcattt ggtcttccaa
     4021 ataggaaagc acttaatgct tcttttctaa cattagcttt aggggcaggg cttgatttag
     4081 caattataaa tccaaatgaa tatagtatga tggaagctat caattctttt aaaatattaa
     4141 ataacacaga taaaggatgt ataaactata taaatcaata tagtaatata aataattcaa
     4201 aaaaatcgga ttcatctaca aagattgaca aagatttgcc attggatatt ttagttgaaa
     4261 gaggattaaa agatgaagca aaaaatgtaa cactgaatct tttaaaagag aaggatgaaa
     4321 attacatatt agatgagatt ttaattcctg ctcttgataa agtaggaaaa agatatgata
     4381 gcggagatat atttttacca caattgattc aatcagcaga aacggtaaaa gtttcattaa
     4441 atacaataaa ggaaacttta ttaagtaaaa gtagcaataa tgtatccaaa ggaaaaataa
     4501 tagttgctac agtaaaaggt gatatccatg atattgggaa aaatatagta aaaataatgc
     4561 tagaaaatta tggatatgag gttatagatt taggtaaaga tgtccctatt gaagaagtag
     4621 tggaagttgc taaaaagaga aatataaaat tagtaggatt aagtgcactt atgactacga
     4681 ctgttcaaag tatgaaagat acaattcagg cactgagaga taatgagata aatgcaaagg
     4741 tatttgtagg aggtgcagtt ttgacagaag aatacgctga agaaatgggc gctgattatt
     4801 attcaaagga tgcaaagtct gctgttgaaa tagcaaagtt aaatttttag catataaatt
     4861 aaaataaagg ttttactaaa taggatattt tatatataat ataaatataa gttttagata
     4921 cttatgttta aaacaatagg aattaatcta taataatgag attaattaat ataaaaaata
     4981 tttatcatca acattaaaaa gggctgggaa gtctggtata acttccatta gcccttttta
     5041 gttaaaatca tatgttaaaa agcccaatta ccatttttaa aaatctgtat ttgttttcca
     5101 tcataagtct caccaataat atccatatca gcagaaccaa tcataaaatc tacatgtgtt
     5161 aaactgtcat ttacaccatg ttcatctaat tcttcatctt ttaaattgtt tccaccttcc
     5221 agacaagtct tataagcaga accaattgca aagtggcaag aagcattttc atcatataga
     5281 gtattataaa aaattatatc cgtattggat attggtgagt tgtatggaac aagagcaact
     5341 tctcctaaat aatgagagcc ttcatccgtt tcaataagtt tttctagtgt atctaatccc
     5401 ttttcagcag agaaatctat tattttacca tctttaaatg ttaagaagaa attgtcaatt
     5461 atgtttccac cataaacaag aggttttgta ctataaacag tcccatttac tttaaactta
     5521 tgtggcattc caaaaatctc ttcagttgga atattcggat taaaacttat tccattaggg
     5581 tctttggatg ctccacttag ccaaacatga ccctttggca agtctagagt caaatcagtt
     5641 tttgaggatt tatattttaa agttttaaag ttatttttat ttagaaagtc cattttagat
     5701 ttaagatttt cattatgttc ttcccatgct tttatagggt cctgaccatc tactcttgaa
     5761 catttaaata tagcatccca cagtttttct attgctttat cttctgataa atttggaaac
     5821 actttttttg cccaatcttt tgttggcaca gagactatac tccatttaca tttatcagtt
     5881 agagtgtaag aacgccattc ttttaaagct tttccagatg ccttctgaaa atttgcaact
     5941 ctttcagggt caacatcttt taataaatct ggattttggg catagactga taaaaatgcc
     6001 ccaccttctt ttgctatatc tacatattgt tctgctgtcc atttggggaa ctcatgaaat
     6061 gcttcatctg gagcatacaa gtatttaata agagtacttt cttcatcaga ccactctata
     6121 taaacatttt tagcaccaca tttatatgct tctttaagtg catttcttgc aaaatcagca
     6181 cattctattg gtgactttat aagtagaatt tgacctggct gtatgttaac tccaactttt
     6241 attgctaatt cagcgtattt ttctaaattt ctatttaaat tcatatttat cctcctatat
     6301 ttttaatatt gaataactta taaagtatta taacattact tatgttttta atattgaaca
     6361 acttataaaa tattataact ttacttagat tttgggtcat attcaaaata tttatacttt
     6421 atagattgag aaataaaaaa aatattttaa ttaaaacaga ttatgaggat aacaaactta
     6481 tttaagcagt atgttttttg ttgataataa tattaaagta gaaataaaaa tactttttta
     6541 gtgaaaagct atacaagaat accgaaaaaa acttataata taataatgag atagatattt
     6601 ataaatgaaa aaaatttttc tggcaaagct taattaaaaa ggtaatagct agtgttgtta
     6661 tttaaattag catagaaaat caaataaaaa aattatgcta atgtatagac attgataagg
     6721 tagaatttaa gccttttgaa tgctatatta aactagagta gatattctta tgaatttcaa
     6781 aatagggcag gaggaaaaag gaaataaaac tataaaaaat ataaagtatt tatataaatt
     6841 tatagagttt atacgtcaga agatttcagt taggagggca taaatggagc tttatgaagc
     6901 cattttttat agaaaatctg taagaaattt ttccagtaag aagataaaaa agccattgat
     6961 ggaagaaata aaaagaagtt gtaaaaattt agaatactta aatgaagatt taaatattaa
     7021 agcacatgtt gttgatagag gtcatataat acattttttg atgggtaagg aatgtaaagt
     7081 aaaggcacct cattatatag tagttacatc agacaaaggt gaggactatt tacagaatat
     7141 aggatttgct acagaaggtg tagttttgcg ccttacaaca ttgggaatag ctacttgctg
     7201 gttggaagga aacttaaaga gagaagatat cttggaattt gtagatattg ggaataagaa
     7261 ttttgaagaa gatgaggaag atgatttaga ttatctagct ggagtaaaaa cagaagaaga
     7321 tgatgaaaac aaaatagaaa atccttatat aataatagca tttggatatc cagcaaaaaa
     7381 tgaagagttg tttaggacga gatatgctag acctgataga aagccagtaa aagatatgat
     7441 aaataagata aataggaata gaaagtggaa agatatctta gaattgacaa gaagagctcc
     7501 atctgtaaaa aattctcaac cgtggtattt ccataaggat gaacgaggtc ttcatttatt
     7561 tgaaaagaga cctaagaaac actgtgaaga tatgaataag gttagcttag gtgtagcctt
     7621 aagacatttt gatatagctt gtataaaaaa taaaatagat gtttcatatg aaaaacttcc
     7681 tataaggaat aaaataggaa aatcatattt tataactgta gtcgagcatg tgaaaccaga
     7741 agaagaaacc caagaagaaa atgtaactct agaaaaagaa gaatcacaag atgaataaaa
     7801 ttttggaagg ggatattgga tatgtataag tgtggaaaat gtaatcttag aggctgttct
     7861 aatggaaata tggataaatt aatgaagata tgtccaacta gagaaattga actacaacaa
     7921 aaagcaaagc aactttatga agaagaagag aatagaaaaa tagcttataa tgctggatta
     7981 gttgaagcag aggggtattg tcaatatact agactaaaag agattataga gtttgctaaa
     8041 aagtgtggtt ataaaaagat tggcatagca ttttgtattg gactaaaaag agaaatgcaa
     8101 acagcacaaa aaatatttga acatcatggg tttgaagtat tttctgttat atgtaaaaat
     8161 ggggcaatgc ctaagagtac aattggacta gaagcatctc aaacaatatc aaagtgttca
     8221 gatgaggtaa tgtgcaatcc aataggacaa gctttatttt taaatgaagc taaggttgac
     8281 cttaatgtaa ttcttggatt atgtgttggt cacgatactt tagccatgaa atatatggaa
     8341 gctcctataa caactttagt tgtcaaagat agagtaactg ggcataatcc agttgctgca
     8401 atatataatg cagaatcata ttttgagaaa aaattgtttg ataaagatga agaataattc
     8461 aaagacttat tgacataaaa agcaattagt ggtttaatta taaaaaagat aaaactttaa
     8521 attatcctat agatacataa agttatataa ataaagaaaa atattacagc taaattcctt
     8581 aaagtagtcc agagagacta aagggttata gataatgtta gtatataacc tctagtaaat
     8641 cttctagagg tttttgatga gtataaaata aaaatttaaa tataataaag tcttttaaac
     8701 tagtccagag agactgggaa agatatctca tagctaatga atggagttat gtatagataa
     8761 aatccttatg cctcttttgg tgtgaggatt tttttatgat tatttaggct ttattaaaaa
     8821 ggagatgtaa aaatgataaa tggtaaagaa aaattgatag ttgcaattga tactgacgaa
     8881 tttgataaag ctaaagaact aatagacaga ttagaagata gtgtagacat atttaaggta
     8941 ggtctagagc aatacgttgc cacaaaaggt aaaacaatag attatcttaa agaaaaaggg
     9001 aaaaaaatat ttttagacct taaattccat gatataccaa atactatgaa aagtgcagtg
     9061 agagctgcag tgagagataa tgtttggtta atgacaatac atgtatcaga tatggaagga
     9121 atgagacaat gtgcactaat tgctagggaa gaagcagaaa gattaaatat agaaaagcca
     9181 ttagtagttg gagtaacagt tttaacttca ttaagtaacc aagatttaca ggacatagga
     9241 tgtaatatga caacagaaga gttagctata aagagagcta aactggcaaa agaatcaggc
     9301 atagatgggg ttgtttgttc agcgcaagaa gtagataaaa tagtagaagc atgtggagct
     9361 gattttataa cagtttgccc aggaataaga cctaaatcag cagaggttgg agaccaaaaa
     9421 agagttgtaa ctccatcaga tgcaataaaa aaaggtgctc attatttagt tgttggaaga
     9481 cctataacaa aagcagaaaa tccaagagaa tcagctatta atattgtaag agaaatagaa
     9541 aatgcttaga taaaatattt gggaggtagc ttatatgaag gggaaattaa ttttagaaga
     9601 tggaacagta tttgaaggaa aagcatttgg atatctaaag gaagctattg gagaagttgt
     9661 atttaatact tcaatggctg ggtatggaga aatattgaca gacgcaactt attatggaca
     9721 aatagttact atgacttatc cacttatagg aaactatgga ataaatttag aagatgttga
     9781 atcaaataag gtgcaagtaa aaggacttat tgtaagagaa aaaagtgata acccaaataa
     9841 ttttagatgt gaaatggata tagacacata tctaaagcaa aataaggtaa taggacttga
     9901 aggaatagat acaagagcat taactaaaat tattagaaac aatggaacta agagaggaat
     9961 tataacttta gaaaatgtat cattacaaga tgttcagtct aaattagaag agcattgtaa
    10021 caaagattca gtaaaaaatg ttacaagaaa agaaattgaa aatataaaag gtactggagc
    10081 taaagttgta gttatagact ttggtgttaa aaatagtgta ctagagtctc ttaaagcttg
    10141 taactgtgat ataactatag caccagcaat gactacagta gaagaaattt taaatataaa
    10201 tcctgactta atcttattat ctaatggacc aggaaatcca gaggatataa atgatatagt
    10261 tgaaaatata aaaaaattaa taggcaaaaa gccattgaca ggaataggtt taggacatca
    10321 aattttagca ttggcattag gaggaaagac ttcaaagctt gtatttggac atagaggagg
    10381 taatcaccca gttaaatgtt tagaaactgg aaaggtattt ataacttctc aaaatcatgg
    10441 atataatgtt tcagaaatgc cagaaaatac agaggtaact cacataaatt taaatgacaa
    10501 tactatagaa ggaatgagac ataaggaatt accaatatat agtgttcagt acattccaga
    10561 atacacaaca aaagaagatt tagattgtat atttaataaa ttcttagcat taatatagat
    10621 ttagtattat ttacagatag taaaaaccat ttagataagg agaaaaattt atgcctaaat
    10681 tagatagtat aaagaaaact ttagtattag ggtcaggacc aataataata ggtcaagctg
    10741 cagagtttga ctattcagga acacaagctt gccagtcttt gaaagaggaa gggattgaag
    10801 ttgtattaat aaatagtaat ccagccacta taatgactga taaagaagtg gcagataaaa
    10861 tatatataga accattaaca atagaattta tagaaaaaat aatagaaaaa gaaagaccag
    10921 acagtttact tgcaggtatg ggtggacaaa cagggctaaa cctagcagtt gaactttatg
    10981 aaaaaggcat actagataaa tatggagtaa agataatagg tacttcagta gaatctatca
    11041 aaaaaggtga agatagagat atatttagag aagtaatgaa agaaataaat cagcctgtaa
    11101 tagttagtga tatagttaca gatttacaag caggtcttga ttatgcactt acaattggtt
    11161 atccagtagt tgtaagacca gcatacacac taggtggaac tggtggtggt atagctgata
    11221 atgaggaaga attaagagaa atactttcac atggtctaca attaagtcct gttggacaag
    11281 tattaattga aaagagtata aaaggttgga aagaaataga atatgaggta ataagagatt
    11341 caaagggaaa ttgtatagtt gtatgtaaca tggaaaatat agacccagtc ggagttcata
    11401 caggagacag tatagttgtt gctccaactc aaactttaag taacaaagaa tgtgcaatgc
    11461 ttaaaaaagc atctttagat atattaaatg cagtagaagt tcaaggtggt tgtaacgttc
    11521 aatttgcact taatccacat agttttgaat atgcagttat agagataaat ccaagagtta
    11581 gtagatcatc agctttagca tcaaaagcta caggatatcc aatagctaaa gtttcatcaa
    11641 aaatagcttt aggttatact ttagatgaga tagaaaatgc agtaactaaa aaaacttatg
    11701 cttgttttga accaactata gattatgtgg tggcaaaaat accaaaatgg ccttttgata
    11761 aatttaaaaa agcaaataga aaattaggaa ctaagatgat ggcaactggt gaaattatga
    11821 gtataggaag taactttgag gctgctattc ttaaagggat aagatcactt gaaacaggaa
    11881 aatattcatt agttcacaca ccatctgagg agagaagcat tgaggaatta aagaaaagag
    11941 ttgtagtacc tgatgatgag agactatttg acttagctga aatgataaga agaggctata
    12001 aagtagagat gatagaacaa attacaggta tagataaatg gtttataaat aaatttaaat
    12061 ggatagtaga gcaagaagaa aaattaaaag ttatgaaaat tgaagattta actaaagact
    12121 atcttcttga attaaagaaa aagggattct ctgataaagg tatatctgac ttaatgaaga
    12181 taagtccaga aaaattatat gaattgagaa gtctatacaa tatcaaacca gcatataaaa
    12241 tggttgatac ttgtgcaggt gagatagatg ctatatctcc ttactattat tcaacttatg
    12301 agcaatatga tgaggtagtt gttagtgata agaaaaaggt gatagtatta ggttctggtc
    12361 caataagaat aggtcaaggt atagaatttg actattgttc agttcattgt gtaaaatctt
    12421 taagaaaaat ggacatagaa actataatca taaacaataa ccctgaaact gtaagtacag
    12481 actttgatac atcagataag ctgtattttg aaccattaac agaagaagaa gttttaagta
    12541 taatagaaaa agaaaagcca gaaggtgtaa tactacagtt tggtgggcaa acagctataa
    12601 aattggctaa gtttttacat gaaaaaaata tacctatact gggaacagat tttgatgata
    12661 tagatgcagc agaagataga gaaaaatttg atgatttact agagagacta gatataaata
    12721 gaccaaaagg aaagggtgta tggtcactta acgaaggtat tgaagttgct aatgaattag
    12781 gttatccagt attagttaga ccatcttatg tacttggtgg tcaaggtatg gaaataactt
    12841 ataacgaaga aaaattatct caatatctac aagatgcatt tgatagagac cataaaaatc
    12901 cagtacttat agataaatat ctaactggta gagaaataga agtagatgct atatgtgata
    12961 aggaagatat attgatacct ggtataatgg aacatttaga aagagctgga gttcactctg
    13021 gagatagtac aacaatgtat ccaagtcaaa atatatctga tgaaataaaa gaaaagatag
    13081 tagaatacac taaaaaaata gctttagacc ttaatgtgct tggtatggtt aatatacaat
    13141 ttatagaatt tcaaaatgaa ttatatataa ttgaggttaa ccctagagct agtagaacag
    13201 ttccatatat aagtaaagta agtggagtac caatagttga cttggctact aagtgtatgt
    13261 taggagctaa attaaaggat ttaggatatg gtacaggagt ttacaaagag ccaaaattag
    13321 tatcagtaaa agtaccagta ttctcaatgt ctaaattatc taaagttgaa gtaagtctag
    13381 gacctgaaat gaagtctaca ggagaagtcc taggtgtagg agaaaattta gaagaagcct
    13441 tatataaagg gttcttggca gcaggtagac atatgtctga tgaaagagga gtagtacttg
    13501 ctactgtaaa taatcatgat aaagatgagt ttatagagat agcaaaagat atgaaagaat
    13561 taggatatac ttttgttgct acagaaggaa cagcgaagag tttaagagaa aatgga
//
LOCUS       DAELSY010000108        13298 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.108, whole genome shotgun sequence.
ACCESSION   DAELSY010000108 DAELSY010000000
VERSION     DAELSY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13298
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.108"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..231
                     /locus_tag="KPG09_002727"
     CDS             <1..231
                     /locus_tag="KPG09_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBG0967323.1"
                     /translation="KSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWKVYGDTK
                     QSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            complement(329..1288)
                     /locus_tag="KPG09_002728"
     CDS             complement(329..1288)
                     /locus_tag="KPG09_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0967324.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(1322..2053)
                     /locus_tag="KPG09_002729"
     CDS             complement(1322..2053)
                     /locus_tag="KPG09_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967325.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(2046..2816)
                     /locus_tag="KPG09_002730"
     CDS             complement(2046..2816)
                     /locus_tag="KPG09_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0967326.1"
                     /translation="MFEKIHKRFKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVVFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(2901..3611)
                     /locus_tag="KPG09_002731"
     CDS             complement(2901..3611)
                     /locus_tag="KPG09_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG0967327.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            complement(3791..3943)
                     /locus_tag="KPG09_002732"
     CDS             complement(3791..3943)
                     /locus_tag="KPG09_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBG0967328.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(3969..6125)
                     /gene="feoB"
                     /locus_tag="KPG09_002733"
     CDS             complement(3969..6125)
                     /gene="feoB"
                     /locus_tag="KPG09_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG0967329.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTIIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHVPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            complement(6225..6446)
                     /locus_tag="KPG09_002734"
     CDS             complement(6225..6446)
                     /locus_tag="KPG09_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0967330.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            complement(6459..6671)
                     /locus_tag="KPG09_002735"
     CDS             complement(6459..6671)
                     /locus_tag="KPG09_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0967331.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(7195..7803)
                     /locus_tag="KPG09_002736"
     CDS             complement(7195..7803)
                     /locus_tag="KPG09_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBG0967332.1"
                     /translation="MVDNLIKYIHKSNIAIVVLHEIYGVNNFIKDVCQKYYKGGYDVF
                     CPELLGEKRVFSYLKAQEAYDFFINNVKFDIYKKVEKLINGLKSEYKHVFVVGYSIGA
                     TIAWRCSENSLCDGIICCYGSRIRDYMDVIPTCPILLVFAKYDSFDVDYVSSQLSRRQ
                     NIQIEILDANHGFIDTYSKNYSNAQTEVFNIKEKNFLSKCLG"
     gene            complement(7923..8351)
                     /locus_tag="KPG09_002737"
     CDS             complement(7923..8351)
                     /locus_tag="KPG09_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0967333.1"
                     /translation="MYGVIGATVLVIFVILTAGIMQGKKVDFQLPTVFMIVTFIVSAI
                     SEEIVFRGYIQTRLTGLIKNSVLSSCINAFLFLSTHYPVKWISSGDFSFAILSGFYVI
                     CLILLNFSCDLVYRKTNCLWGAWLLHILYNIGTGVLIFTT"
     gene            complement(8370..8630)
                     /locus_tag="KPG09_002738"
     CDS             complement(8370..8630)
                     /locus_tag="KPG09_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967334.1"
                     /translation="MFKTVFEDKEYVLLCKNYSKSDGVKAIFIYLISMLLLFFQGYLY
                     TTNLSSTILTNSQMILSILILLIGLGFVLLSKEKLNTIGITM"
     gene            complement(8904..9449)
                     /locus_tag="KPG09_002739"
     CDS             complement(8904..9449)
                     /locus_tag="KPG09_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG0967335.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(9567..11390)
                     /locus_tag="KPG09_002740"
     CDS             complement(9567..11390)
                     /locus_tag="KPG09_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967336.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGVLASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(11403..13178)
                     /locus_tag="KPG09_002741"
     CDS             complement(11403..13178)
                     /locus_tag="KPG09_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967337.1"
                     /translation="MKYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLREKIGVVFQNSSVFGKSILENIVMGNEGISREDVIKSC
                     EIANANQFITKLENSYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         4437 a         2432 c         1306 g         5123 t
ORIGIN      
        1 aagagtgatg tttttcctga tgaaataggt atttttttag gatatcctct taaagatgtg
       61 attggattta tgggatatag taattatgaa gtttctatga ttaaatattg gaaagtttat
      121 ggtgacacaa aacaatccga ggacacatat tcaaagtttt tacttcaccg taagaaaatg
      181 agaaaattac tagactacat aagtgtagat aaaatagtaa gttgttttta attgtatttt
      241 tgtataataa taaaaataca ccctctttta ccatacagtt tccataataa ataagggtgt
      301 attttccact ttaatatttt aaagtattct aattttgatt gtacaacata tctttaatat
      361 ttattttctt ttgttgcatt ccattagaca tcaaaaattc ctgtgtatct tctaattctt
      421 ttatatcctt gtccgttatt ttagaactaa agtcatacca agaatacatt tcttttgttt
      481 catctaaaga taaaccaact tctttagata caacatccat cacttcatcc ttgttttcat
      541 tcatatattt taaagtttct tcatgaacct tcataaatct ttttactaat tcaggatatt
      601 tttcagcaaa gtcatcactt acagcagtaa ctattattcc acttactaat ccttctccat
      661 ttacaatcat tttggcacct gaatttatcg ttttcaatgc cactggacct gcaagcattg
      721 ctacatcagc acttttactt tctaatgcag aagcagcact aggaatatcc atatttacat
      781 attcaacatc atctatagta tagccttctt tagcaagtgc agcaatcaac agttgatgaa
      841 gtatagtccc ttttggacct acaatttttt taccttttaa gtcttttata gatttaatat
      901 tatcattatt agttataagc atatatcctt ttggtgaacg gctataaata ttcgtaattt
      961 taagaccaac accatttgat gcagctatta ttgcagacgt tcctcctaat gcatgtaaaa
     1021 agtctagctc acctgctgct aatgcttgag tttgctctgg tcctgttgtc aattcatgga
     1081 agttgacttt tatattgtcc tttttaaatt cctttccaaa taaatcatcc tttttttcta
     1141 atatactagg aacattaagt ggtgatttta cataagttaa atttatttcc ttaggtaaac
     1201 tagcctcttt ttttgcaggt tcatctttct tatcatttgt tgttgaacat cctacagtcc
     1261 caaccaacac cattattaaa gatgtcatta ctgctaaaat ctttttgttt cttttaatca
     1321 attaaaactc ccccaattcc cttaatattt cttttttcaa gtttatataa tagtttttag
     1381 ttaaatctct attatattca tcttcgacac taaattgttt aatcctttta tttttactaa
     1441 aaacaattat tttctttgct atttccatag cttcttcaat attgtgagtt acaaatacta
     1501 cacctttttt agtattttta tggatattta caacttcttt ttgcattttt cttcttgtga
     1561 agtaatctag cgctgcaaat ggttcgtcca tcagcaaaat atcgggattg aaggaaagtg
     1621 ctcttgctat acttactctg tgagccattc ctcctgataa ttcatttgga tatgagtttt
     1681 taaacttttc aagcttcatc atttttaaat acttgtctaa atcaacttta tttctattat
     1741 ctttttcagt atgtaaaagt atattttcac ttacattcag ccatggcata agtctacttt
     1801 cttgaaatac cattcctact tttggagtac actttttatc attattaaaa aaatatatgt
     1861 ttccactagt agcattttca agccctgcta aaattctaag tagcgttgtt ttcccacatc
     1921 cactttcacc taatataaca gttatctctt cactagatat atttaaagat atattatcaa
     1981 gaactaaatg ctctttatta tcaactaaat atttcttata tatattctct agcaaaaatc
     2041 cacttctaac catttaaatc aacctgcttc cctttgctaa attttgttat tatctttgaa
     2101 aatagataat ctgaaagtat acctaaaaat ccaataacaa aaattcctac caatacaaca
     2161 tcagttctag acatatcttt tccatcagaa atcaaatatc ctagtcctga agaagctgca
     2221 ataagctctg ctccaattat agccctaaaa ctatacccaa gccctaaact aagtccaaca
     2281 acaatatcca gcatagaatt agggattatt attttataaa aaattctctt tttagataaa
     2341 ttaaaagatt tacctacttc tattaattta ttgtcacaat tttttatacc tttcaatgta
     2401 tttaaaaata ttggaaaaaa ggatgccaaa ataataatta taattttaga ttcttcacct
     2461 ataccaaacc ataaaataag cattggcacc aaagctagtg gaggtgtact tcgcataaat
     2521 tcaaatagtg gtttgaaata ttcatacatt tttttattta ctccaaatac aactccaagt
     2581 ggtatagcca tcaatgaact tattaaaaaa cctattaata tccttcttac actggcataa
     2641 acatttaaga aaatagaacc gtcatatatc atttttataa aagtatctaa gactatttca
     2701 ggaggaggca gaatatagct actccatata cccaaaacac atactatctt ccataaaatt
     2761 aaaattatta aaaaaatata atatccttta aaccttttat gaattttctc aaacatataa
     2821 aactcctttt aacatatcta tccctgctac cctttttatt ttttaatatc ttaaataata
     2881 attaagctat atcataagat ttaatctatg ccaagtctta atccacctga actcattcta
     2941 tcagaaggtg tagaatctgt ttttaagcca gacaatcttt cagatggttg tataatgact
     3001 atttgtccag gttcaacaaa ttccatatga taagattctc ctgaagtctg accaataaaa
     3061 gtcttccatg atagttttga tactttagga tatggttctc ttccagtcca agctactaat
     3121 gcatctgggt caacacaaca aggtccttca agaattagag gattaccatc tgttattata
     3181 gcaacatctt gattttgacc tgttttattt actaaatgtg tagtaaaaag acctttttga
     3241 gatattaccc ctgaaccaat caattttaca ctgtaattta attgttcaga aaatgccaat
     3301 aagttttcac tctcaacata aatggaatca cctgggtcaa ttgatattac atctacatgg
     3361 taagcatttt gagctaggta aatttcacct tcaccttcaa ccaccattat tggcatttgt
     3421 tctcctgtca atgacctctg aacatgacct aataatgctc ttcccaatcc accgccatta
     3481 cttggaccta acagcaattt ttcaaaatta aaatttcctt taaaggctac catagcacct
     3541 ttttttgcaa aaaatttacc acttcccctt gctatacaca taagctcgtt tgtaacatca
     3601 aatttaaaca taatattcac ttatattgct taatttagca ataatccctc ctaatcatat
     3661 atttatatat aattatacag taaaattaca gcatagtcta acctacttca aatatgaaat
     3721 ttgttattta tataaaaaat aagaacctga taataaagca atttaacttt ttatcaggct
     3781 cttatcctat ttatctctta gcgctatgac aactcttact acttgagcat ccagaacaac
     3841 cacatccaca actgctacca ttcttttttg aatttttaac catatacatt acagctagtg
     3901 ccattaatac tactacaata gctgctataa taaatgtagc catattcatc actccttatt
     3961 tatatgaatt aatttttcat ataacttaat ttacccattt tttctttttt catattttta
     4021 tttgaataag ttaaaactac aaagactact gctactgcta taaatataga tattattgcc
     4081 ccagcaatac ttcctccagt acctaaaact aaacttccaa cttgatttat aagaagagca
     4141 attatatacg ctgttaaagt ttggaatcct aatgtaatcc atgtccattt ccatgaaccc
     4201 atttctcttt ttatggctcc aatagctgca aaacaaggtg cacaaagcat attaaatgcc
     4261 ataaatgcaa acgcactagc tactgtaaac atacttgcaa ctgaagcaag aagtgttggg
     4321 tcttgctctg ttgcatctga tataccaaat aaaactccaa atgttccaac tacattttcc
     4381 ttagctacta gacctgtaac tgttgctact gatgattgcc aatttccaaa tccaagagga
     4441 gcaaatattg gtgcaactat atttcctaag ctagctaata tactatctcc agcatctacc
     4501 atttgtaatg accaattaaa tgattgtaag aaccagatta ctccacaagc aacaaatata
     4561 attgttcctg ctttgattat aaatgctttt cctctatccc acatatgtat taaaacacct
     4621 tttgcacttg gaacatgata ttgaggtaat tccataacaa atggagatgg ttctcctgca
     4681 aataaactag ttttctttaa tataattcca caaatgatta tcattgcaat acctaagaaa
     4741 tacattgatg gagctaccca tgaagcacct ccaaataatg cccctgcaaa taatgctatt
     4801 attggaatct tagctccaca tggaatgaat gtagttaaca taatagtcat ttttctatct
     4861 ctatcattct ctatagtacg agttgacata actcctggaa ctccacaacc tgaacttata
     4921 agcattggta taaatgattt tcctgaaaga ccaaacttac ggaaaattct atccattata
     4981 aaagctacac gtgacatata cccacaatct tctaatattg acaataataa gaataaaagc
     5041 atgatttgtg gtacaaatcc tagtactgct ccaactccac caattagacc atcaactaca
     5101 agtccttgta accactctgc tacatttaaa gaagctagaa aattacttac attgccttga
     5161 ataatttctc caaacagtac atcatttgtc caatcagttg ctatagtacc caatgaactt
     5221 actgctatat agtaaactcc ccacattata agtgcaaaaa taggtaatgc tagtatacga
     5281 tttgttacaa tcttatctat cttatctgat acagtttcct taccttttcg attcttcttt
     5341 attatacttg aaattattga actaataaat tcataacgat tggctgttat tatactttca
     5401 ctatcatcat ccaattcatc ttcacaattt ctagttattt cttcaatact attcaatatt
     5461 gttttagaaa tatcaagttt ttgtataaca ttttcatctc tctcaaatag ctttatagct
     5521 aaccagcgtg tttctacatc tatatatgga gtcttttctt ctattatttt ttcaattttt
     5581 tctattgcgt ccttactttc atcaacaaaa ggtaatttga aatttaattt attgttacta
     5641 cttgcaagtt caactgcctt ttcagcagcc tccataattc cttgaccttt aactgctgtt
     5701 acttctacca ctgggcaacc tataacttca gatagttctt taatatttat cttatcacca
     5761 ttcttattta caatatccat catattaaga gctataatgg tcggtatacc tagctctaaa
     5821 acttgagttg taagatataa atttctctct atatttgatg cgtctacaat atttattact
     5881 gcatccggct tatcatctag cataaaatta cgtgtaacta cttcttctaa tgtatatgga
     5941 gataaagaat aaatacctgg taaatctact atttccacat ctttatttcc ttttaatttt
     6001 ccaccttttt tttcaactgt tactcctggc cagttaccaa catattgcga cgagcctgta
     6061 agcccattga acattgttgt cttaccacag ttaggatttc ctatgagacc tatttttatt
     6121 gacatttcca ttccctccat tattatttaa atacatcact gtttgtgata cagaaaatca
     6181 ttctcattat ataattacat taaccctact ctaattaatc tttattattc cacaataatt
     6241 ttttctgcat ctgacttacg aattgatagt tcataatctc tgactgttac ctcaattgga
     6301 tcaccaagag gagctacttt tcttataaat atattaacac ctcttgtaat ccccatatcc
     6361 attatacgtc ttcgtgtagc tccttctcct tctaactttt tgacttttac agtctctcca
     6421 cacttaatat ccttcagtct gttcatgtca ttacctccct atattattat tctacttgcc
     6481 atttttttat ctaatgcaac tctagtatct tttacgtcta ttattaagtt accatctatc
     6541 ttagaaacta ttttaacatt tgtacctact acaaaaccta tactatttaa aaaagtcctc
     6601 gtcttatcac ttcccgaaat tttctttata actacttctt cacctgtatt tgataatatc
     6661 aatggcatca tataatattc ctcctcttca accaatttaa atttacaaac aacctctatc
     6721 ttatatataa atcacaattt cttaaatttt gactcgttaa aataattact tatctttttc
     6781 cttattctta caattcctaa ttttgattta agtctatcac aatagcatat tttttgcaag
     6841 tgattattgt attcttttga ttttttttct catttgattt attgtattaa taaaatctat
     6901 aaaaattcat actgctatcc tatatatttt gagatttctc ttagatactc ataaccatat
     6961 tctgcataag aatgttcttt tgataaagat attattctat agtaatgatt tttttctttt
     7021 caatgtaatt taaatattaa tctaatacaa tcataaaaac ataattaatt ctatgattct
     7081 actgactatc tctatctcaa aaaatataat gacactacta atatttttta acattagtag
     7141 tgtcattatg tttttcaaat tgattaaatt ttctatattt tataataaaa catattagcc
     7201 taaacattta gatagaaagt ttttttcttt aatattaaaa acttctgttt gagcattaga
     7261 atagtttttt gagtatgtat caataaaacc atgatttgcg tctaaaattt caatttgaat
     7321 attttgcctt ctagaaagtt gagaagaaac ataatcaaca tcgaatgaat cgtattttgc
     7381 aaacactagc aaaataggac atgttggaat tacatccata taatctctaa ttcgggagcc
     7441 ataacaacaa attataccat cacacaaaga gttttcacta catctccatg caatagttgc
     7501 acctatacta tacccaacta caaatacatg tttatactcc gattttaaac catttataag
     7561 tttttctact tttttataaa tatcaaactt aacattattt ataaaaaaat catatgcttc
     7621 ttgagctttt aagtatgaaa aaactctttt ttctcctaag agttctggac aaaacacatc
     7681 atacccacct ttataatact tttgacaaac atcttttata aaattattaa ctccataaat
     7741 ttcatgcagt acaacaatag ctatattact tttatgtata tacttaatca gattgtccac
     7801 cacctgtcga tattttttac atctagttta aattatttta agtatgtcaa tttactagct
     7861 ctaaaatttg acttataaaa taatccgttt cttacgataa actttctgca aagtaaaatt
     7921 gactaagtag taaaaatcag cactcctgtg ccaatgttat acaagatatg cagtagccat
     7981 gccccccaca gacagttagt ttttcgatat actaaatcac atgaaaagtt aagtaatata
     8041 aggcatatca cataaaatcc agatagaatt gcaaatgaaa aatctccact tgaaatccat
     8101 tttacagggt aatgtgtaga tagaaaaaga aaagcattaa tacaagatga aagtacacta
     8161 ttttttatta atccagttaa ccttgtttga atataccctc taaaaacaat ttcttctgaa
     8221 atagcagaaa caataaatgt aacaatcata aaaacagtag gtaattgaaa atcaactttt
     8281 tttccttgca taattccagc tgttaaaata acgaaaatta cgagtacagt agcaccaata
     8341 actccataca aaagactttt atttaaattt cacattgtaa tcccaatcgt atttagtttt
     8401 tccttcgaaa gtagtacaaa tcccagccct ataagtaaaa tcaagattga caatatcatt
     8461 tgtgagttag ttaaaattgt tgaacttaaa tttgttgtat acaaataacc ttggaaaaac
     8521 aatagtaaca tagatatcaa ataaataaat attgctttta ctccatcact tttagaataa
     8581 ttcttacata ataaaacata ttctttatct tcaaatacag ttttaaacac tataaatact
     8641 cctttctaat taaattctta tttatttctc atattttatc ataaatatat gtttaaatca
     8701 aattaattct atttattaca taaatcagag ataataaaac taaaaaatta atttcattaa
     8761 aactatattt ataatgattt aaatatttac aagattatag ctataaaaaa tataaaatac
     8821 aactattaaa aaggagcata tcattataat tttaaaaatc ataaatgata tactcctaaa
     8881 aatctactta acttatatta aatttatttt ccaaaatatc tatttaataa tcctaagaat
     8941 gctttaccat gtctagcttc atctttacac atttcatgta ctgtatcgtg tatagcatct
     9001 agtcctaatt ctttagcttt tttagctaat tttaattttc catcagttgc accatactca
     9061 gcatcaactc taactcttaa gttttctttt gtatctgcaa ctacaacttc tccaagaagt
     9121 tctgcaaatt tagcagcatg ttcagcttct tcaagagcta ttctcttata agcttctcct
     9181 acttctggat aaccttctct atcagcttgt ctactcattg ctaaatacat tcctacttct
     9241 gtacactcac caacaaagtt agctcttaat ccttcgatta tttcttcatc tacaccttga
     9301 gctactccta ttctgtgctc atcagcccaa tttttttcgc ctttcatttc ttcaaattta
     9361 tcagctccaa ctttacatac tggacattgt gctggtgcag catctccttc atgtatatat
     9421 ccacatactg tacaaacaaa tttcttcata attactttcc tcctaaattt tatatatcaa
     9481 attaaatttt ttatttatta agttagtcta tatatacccg caccatatac ctttaaacaa
     9541 tttttttaaa agttttttct aaatacttat tttccctgtg tcaataatat gtttctataa
     9601 taatgtccct ttaattttat caattcttta tgatttcctg attccacaat tctaccattt
     9661 tctaaaacta ttatattatc acaatttgat atggtactta atctatgagc tataattata
     9721 cttgtctttc cataagttaa attatttatt gcttcttgaa ttttagcttc tgttgctata
     9781 tctatgttac ttgtagcctc atccaaaata agtattggag cattttttaa tactgctcta
     9841 gcaatactta ttaattgttt ttgaccttca cttaaatctt cacccctatc cgataataca
     9901 gtactatatc cttcacgcat tttaactata aatttatgtg catttgctat tttactagca
     9961 tatataactt cttcatcagt agcctccaga tttccatacc ttatattttc cataactgta
    10021 tcattgaata tataaccttc ttgtggaact actccaaaac aagttctcaa atagtttctg
    10081 ttaatagagt atatatcctt accatctaaa aatatacttc cttcatctat atgataaaaa
    10141 tttgtaatta gatttataat cgttgttttt ccacttcctg tctctcctat gatggcattt
    10201 gatgtgccag ctttgatttt taggtttata ttatctaaaa ttttcttttt tgaatcataa
    10261 gaaaaactta catctttaag ctcaatttct ccctttacaa aatgagagtt taaattacca
    10321 tctttattat cgttaatctc acttttaaac atgttatgca cttcttgctc ttcttgttca
    10381 tctataattt caaaaactct ttcacatcca gctatggctg aaagtaatgt attaaataga
    10441 tttgctaatt catttaaagg tcttgtgaat tgtcttacat aacttaaaaa actagctata
    10501 acccctatgg ttataatatc tttagatgca agaacacctc ctataataca tatagttgca
    10561 aatcctatat tactaatcac attcataata ggcattaaaa gagaagtgta aatttgcgat
    10621 ttaagggaaa tatttagaag tctatcattg acttctttaa aatcatttac agattttctt
    10681 tcataattaa aagattttac tacatttatg tttgatatca tttcttcaat atgtgcattt
    10741 agttttccca actcaacttg ttgttgctta aaaaaaactc tagttctctt tgcaataaaa
    10801 ttactcacaa acattactat cggtattaat attacactgc aaaatgttaa aattgggctt
    10861 aatacaaaca tcataataaa agtcatcaat atgtttaaga ttcctgtaat taactgtact
    10921 aatgaatttg atattcctgt actgatatta tcaacatcat ttgtcactct actcataata
    10981 tctccagtag aacttttatc aaaaaaggtt attggtagat ttttaaattt taaaaataaa
    11041 ttacttctca tttctttaac tatactttga gataatctag ctgtacatat ccccttagac
    11101 agtttaactg aaaactcaat tatataaaat gaaagtaaaa taattattcc ttttaagaaa
    11161 atggattttg aataagtatc gatatctatt atgtcaacta cctttccaat agtatatggt
    11221 attacagttg atataattga atcaattatt actagcaaaa aaataaatct agtaatcttc
    11281 ttatcttttt taaagtacat aaaaattctt ttaaacgttt ttttaaaatc tttaggcttt
    11341 tcttttttct ttaatctact gaccttttga tttccagtac ttaagttcaa tatacttcct
    11401 ccttaattta catcttcact tactaaattc tgcaacgcaa aaattttttt ataaattgga
    11461 gaactaacca gtaaatcttc atgtgtagca aatccaacca cctcaccctc atccaagact
    11521 aaaatcttat cacagtcaat tatagaacta atcttttgtg aaatagttat tacagtacat
    11581 ttcttacttt ccttttctct taaattactt atatactctt ttatattctt tcttataatt
    11641 gtctctgtat taatatccat tgcactaaaa caatcatcaa gaattaatat ttgtggtttt
    11701 ttggctatag ctctagctat agtaattctt tgtctctgtc ctcctgataa atttttaccc
    11761 ctttgaaata aaacctcatc ataagaattt tcgagtttag ttataaattg atttgcattt
    11821 gctatctcac atgattttat aacatcttct ctacttatac cctcatttcc cataactata
    11881 ttttctagta tactttttcc aaacacactg ctattttgaa atactacacc tattttttcc
    11941 cttaaacttt cttcagaaat actgtatata ctacgtcctc caatcttaat atcaccttta
    12001 tcaattatat ataatttttg aattaaattt actaaagaag tctttcctga accagtttga
    12061 ccaatcacac caatattttg accttcttct attttaaagg atacgttttt taatattttt
    12121 tcatctgtat ttttgttata cgaaaaatct acattttcaa aaattatatc acaatttaca
    12181 aactttaaat ctttttctcc aacatctaaa ctttcttctc tctcaattac ttcttcattc
    12241 tcatcaacca aaacctcact tattctctca taagatgctt ttgccctaac aaaaacatta
    12301 aaaacatgtg aaatcattat aagagatgtt aatattctag tcatatagtt tacataagca
    12361 actataatcc caattttaat ttctcctgaa taaattaaat gccttccaat cagtaatatt
    12421 attattatgg aaacattaat gattattgta attgtcggtc taaaaaatga catcatagtt
    12481 gtagcttttc tactattttt aaaaagttca tcatttaact cttcaaagct ttggttctca
    12541 taatcgtatc tacaaaatag tttaactact tttattccac ttaaaaactg tcttaatttt
    12601 gaatttactt tatctgtact ttcttgtaca tttttaaata atctataccc tatcttcata
    12661 ttaagcaata ttataataaa tataattaaa actgcaatta gaataattat tgacatcttt
    12721 aaatttaatt ttatagctat aattaaactt cctatacaca ttattggtga ctttataaaa
    12781 actctcatca ttccattaac gaattgttgc atttgattga tatcattagt aattcttgtt
    12841 attaacgaag ctctaccaaa tttatctatt tgttttaatg agtatgtatt tatcttttca
    12901 aatacatcct gtcttaaatc ataagaaaaa tgctgagaaa ctcttgccga gattacactt
    12961 cttattattg caaatattgc acctaaaaaa gttataccta tcattatagc tcctgttgat
    13021 agtatgtagt ctaggttctt atttgaaacc cctatatcta ttatttttgc cattatagtt
    13081 ggttgagcta aatcacaaaa agcttccaat gttaaaaaaa atactgctgt tagaaataat
    13141 actatatatt tttttatgta ttttttaaaa tacttcattc aatcatccct tcatagaatt
    13201 ttctcattac caaaagtata tcataatggc taatttaatt gattatatca attataaata
    13261 tttgatttat tattaatttt tataccatat attttata
//
LOCUS       DAELSY010000109        12930 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.109, whole genome shotgun sequence.
ACCESSION   DAELSY010000109 DAELSY010000000
VERSION     DAELSY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12930
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.109"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(119..280)
                     /locus_tag="KPG09_002742"
     CDS             complement(119..280)
                     /locus_tag="KPG09_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003489153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0967338.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFD"
     gene            complement(454..1320)
                     /locus_tag="KPG09_002743"
     CDS             complement(454..1320)
                     /locus_tag="KPG09_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0967339.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNVEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTREYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(1546..2415)
                     /locus_tag="KPG09_002744"
     CDS             complement(1546..2415)
                     /locus_tag="KPG09_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0967340.1"
                     /translation="MDYKTIILNRKSVREYKNIDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNKSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(2921..3142)
                     /locus_tag="KPG09_002745"
     CDS             complement(2921..3142)
                     /locus_tag="KPG09_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967341.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(3643..4623)
                     /locus_tag="KPG09_002746"
     CDS             complement(3643..4623)
                     /locus_tag="KPG09_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967342.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRVYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(4787..5659)
                     /locus_tag="KPG09_002747"
     CDS             complement(4787..5659)
                     /locus_tag="KPG09_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0967343.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(5823..6374)
                     /locus_tag="KPG09_002748"
     CDS             complement(5823..6374)
                     /locus_tag="KPG09_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0967344.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSYVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(6394..7296)
                     /locus_tag="KPG09_002749"
     CDS             complement(6394..7296)
                     /locus_tag="KPG09_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG0967345.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRTQINNKCDLCGECIKVCLKDAICIMT
                     EKMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(7311..9683)
                     /locus_tag="KPG09_002750"
     CDS             complement(7311..9683)
                     /locus_tag="KPG09_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBG0967346.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAEGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(9902..10591)
                     /locus_tag="KPG09_002751"
     CDS             complement(9902..10591)
                     /locus_tag="KPG09_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967347.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMAGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEEFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(10832..12211)
                     /locus_tag="KPG09_002752"
     CDS             complement(10832..12211)
                     /locus_tag="KPG09_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0967348.1"
                     /translation="MLNFMNELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGSYFSNINYLFYILAFIAWGGYYNYFYKMDYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPHLESIKIQIYSNLIIILIYLVFSFFRKS
                     IKLISQDKRNYVYLLFALLANTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEICLKKGIKFTCS
                     INFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENINKYIRIKGTITKSFFVLKCEN
                     SKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPL
                     EQDTDSWGD"
     gene            complement(12205..12918)
                     /gene="rgbR"
                     /locus_tag="KPG09_002753"
     CDS             complement(12205..12918)
                     /gene="rgbR"
                     /locus_tag="KPG09_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBG0967349.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDDEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNAAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
BASE COUNT         4226 a         2241 c         1223 g         5240 t
ORIGIN      
        1 tagcaattca agtagtattg gataggttct atcctatctc tacatataag tttccctata
       61 tgctgactat tttagctact aactcatgac taaagtcaca agtgtgcgta gctatttttt
      121 aatcaaagta tctagctatc ttaaaggcta agatgataga aggattatac ctaccatttt
      181 ctaatgaacc tatagtttgt cttgatactt gcaaaataga agcaagttct tcttgtttta
      241 tgcctctttc ttttcttatt tcttctaatc tatttttcac atctatcccc ctttaatatc
      301 tcaactacgg aaagtctact ttccataatt atacaactat atttcattta tgtaaagctt
      361 gctttccata tttacaaaaa aataacagac tattttcagc ctgtaatttt aaaatttcat
      421 gttgtttatt aaatttaatg ttattcatta actttaactt ggtttctgaa ttggaatcca
      481 aacttctgta gtataatcag gagcattgat atcgccattg ggataaagtt caatatctgg
      541 taagttagca tacacatacc cagaacttgg taaccactca gtaataattc ttttttgcaa
      601 ttcctgtatt gccagagcat ttggcattgg agaaacacat tcaaatactg cccatgtacc
      661 tgatggtatt aagtattcac gagtatcttc taatacaggc ttatctgttg gtgcagcaat
      721 ataataatct aaacccttac tatttgtaca cacactaact ccaagcaatc cacaaggttc
      781 gttatttatt aactctgaaa gtttatcaat tgttccattt tttatgcatt tagcccataa
      841 cttaggaact ttctcaaagc actcttctac aatcatgttc atactttctt ttataccaac
      901 aatacgaaac cctttctttt ctacaatacg atattgcatt tctacatttc cttttacata
      961 aatgcttaat gtcatcttag gatatgcttt taaaacaaca ccctttgctc tagctgcaga
     1021 tggagaaatg ttatgtatat tttgaaaagc tctattaaaa gatgttggtg aatcgtaacc
     1081 atacttctca gatagttcta aaattttgat gttactgttc tgaagttcaa atgcagcctt
     1141 agtcatacgt cttcgtctaa tatattcaga taatggtact tctgcaatat atgaaaacat
     1201 tctttgaaaa tgaaaggttg aacaacatgc aatttttgct gcttctgcat aatccactcc
     1261 actttcgaga ttttcttcaa tataagtaat agcttgattt aatttattta tccattccat
     1321 ttgatattac ctccaagcaa attatacagt ttaataaaat aatatacctc tctattcatg
     1381 cacaaaattg taagcattta aattcataag tatttttcaa tcaatacaat aaatataaaa
     1441 attgtattat acaataaaaa agttcaataa cattttatta taaaatatta ttgaactttt
     1501 tataaaaatt aatttaagtg tagttaaatt aagtattaaa ctatcttata tattacaata
     1561 tcctactatc ttatagtctt ctggcacttt ataatctttg tctggagtac ctaaattcca
     1621 ttttaaatca aacatagttg cacttattat tgttgcaaaa taaagcataa ctataccaat
     1681 gtctattttt tcttcatata aattagtatg accatctttt ctaacagcta aaactacagt
     1741 gcctccatca actataaatc tccatggttg tctatttaat gtagatggag ctaatcttgc
     1801 atagtgaaat gcatcaagaa gtgctctttc ttctaagatt tctatacttg caacatttcc
     1861 ccattcgttc atataaacaa catcttctac acctagtcta gtagacccct tatctgttac
     1921 aacttgcata tcagattttg aataattttg cccagttttt gtagcattta atactttagc
     1981 attacttaca ttttctccat atccaatagc tataattcct gttacctctt tatctgaatt
     2041 aattccaagt ttttctttta ccttttttcc atcttcaaaa gtaacccaac aagaaccaat
     2101 tccaagttct gttactttaa agattatact ttctcctatg taccctgaat tttcaatgta
     2161 attatctttt atgtcagata atattataat ataacttgga gaatctatca tatttccttt
     2221 gtaccctgct actttatcta aattattaaa aacttcactt ttattcataa ttcttacatc
     2281 aacatctatt tctggaacta gtttcttaca agattctgag tatttcttaa tttcactaag
     2341 atattcttct ttaatatcta tgttcttata ttctctaaca gactttctat ttaatatgat
     2401 tgttttataa tccataattt tatcctcccc aaactagtat tgtcttaatt atatacccat
     2461 taatatatta ttttactcat ttatttaata aataaagatt acaattttgt aattattatt
     2521 tattaataat attccacttt tacattaata atgaccagtt gtgcaaaatc cattgccaac
     2581 caccaaataa aaacatatta taaaagtaat ataaaataac aaacaaatct taacaaaaaa
     2641 ttaaactttt tttacacgaa agaaggcttc ccgttgcctt ctttattttt ataaaaaaat
     2701 aagctgtttc taaaatgatt tcacattttt gaaacagcca agttattcta taatgacaac
     2761 tataatttga ggatgtgagg gagagttgtc attataaatt tagggtaatt atattatgtt
     2821 ttaagctagt tcttcctata tttatataat acattttata tatgaatttt atatgagatt
     2881 ggtataaatg atatatgaaa ttacatatgt ttatattata ttaaatttta ttattacatt
     2941 ttccacaaca atactcatat gtctccacat tatttggaat atttacaaat acaaattctt
     3001 tacagttctc acaatacttt aatatagata tagttccaca agtgtaacat tcaaatatta
     3061 cacaatcctc attttcctct actatttcaa actttttact atgttcacca cactctgggc
     3121 acttgtgatt atttacattc ataaatactc ctccttatta gtatagttac atttcaaaaa
     3181 cttaattaat gtattctata ttacttatta tatattctat aataaatcat attctaatat
     3241 aataaaacaa attaaataaa aaaaaaccat aaattttaaa tttatggatt aattcttgta
     3301 atgataactc tatttgagga tgtgagggag ggttatcatt acaatagggt attatattat
     3361 ggtttttaag ctagtctttc tacacctcat atgatacata tgctatatga attttatatg
     3421 atttttacat aaatatttta tgaaatttaa attttttagt cattttgaaa tttgtaaaaa
     3481 gttctctaaa aatccacagc atcataaaca gccaaaattt aagtaaaaca tcataaaaaa
     3541 atttgtccaa taattttaac aagtcatttc acctcattat aattattgac acaaacttaa
     3601 ataaacaatc tataaatctt atataaatct taaactttac ccttattgat acacaatttc
     3661 tagtttgtca attttatttt gcatctctaa gaaaatttct tcaacagaat tgttaaatat
     3721 attgcctaaa tagactcttt tatctttttg aaatgaataa gattttacac taccatcaga
     3781 agaaatatat aaatgactcc actctgctcc atattttgaa aacctctcct ttgtatcttt
     3841 aaacctcata tcttctattt ttattgcttt ttctctgtct cctcttacta cagtgtgacc
     3901 cctttttatt aaactggtta tttttatatc ttcttttaat ttaatacgtg tatcatcatt
     3961 ttttgaatat actttccata tcactttatg acgataatta tctaattctt ttattaagct
     4021 tctatcaata gtaaagctat ctttagttac cacaattaaa tcaaaatggt ctaacatata
     4081 ttgaaatatt tctctcatac ttttattttt taaaatatcc tcaccaataa cctctataaa
     4141 tctacagcct ttctcataca tgcagttcaa taaacttgtg atttcagaaa aataatcatt
     4201 tgtattatta tctccatcta tttctatagt aaagtacaaa ggagtaatgt atgatttatt
     4261 tcctgttatc tcaaatacac ttgatagtgt ctgtatatca tcttcaaacg ttaagtatac
     4321 attctcataa aacataaaaa attccataac tttttctttc atattatgga tatttctata
     4381 tctattggat aatatattta ttatatcgtc agtagtattt ttcccatcac attcagccag
     4441 tataacaatt tgcttccaac ttaagctatc cacatttccg atactttttt tatatccatc
     4501 ttcttcatca taaataggac caattataat gttttctttt ggccacagat gaaccctagt
     4561 tccatctttt aatttcggat atttattttt tacagactca taaaatctac ttgtatcacc
     4621 catgattctt tactcccccc catagtttaa atattctatg ctaaaaatag cactttattt
     4681 ttattaattc aacaattatt caaaattggt cattatatat gtttaaatgg atttaacatc
     4741 aaccttattt atatgttatt gatgtttata tatgttatat aaaatcttat ttctcatact
     4801 tcaatgtaat cttaactatc tctggtttac aattaagcct aattaaaact tttgagcctc
     4861 ctaagccttt atttaaaatc atagtgctat tatctttttc atattttccc cagtcatact
     4921 taggaaagaa ctctctattt ggagataata cacctccaac aaaaggtatt cttattatac
     4981 ctccatgaac atgtcctgct aaaaccaaat ctgctcccca ttttgcatat ttttcaaaga
     5041 aaaatggtgt atgtgctaaa agaatgttat attcattttc attaacttta cctagattgt
     5101 cttctacaaa attgtcactt agttctaatt ttttgttatt agcaaataaa tatggatagc
     5161 attccaatgg tataattaaa ccatataaat taagatggct attacctcta tatattctta
     5221 cagattcatt ttccatattt attattttct ttgaatatag ttccttaaaa taatccttat
     5281 atagatgttt atactttttt attaaagatt tttgttcatg atttccaatt atatgatata
     5341 cttcatattt ttcaaccaaa ttatctatta aatctaatgc aacttgaaaa tttttatttt
     5401 ctccatctac taaatctcct gttataaata taacatctgg gtttaatgat tcaattttat
     5461 ctatcaatac cttattattt ttatcaaaca ccttattatg taaatcactc acttgaacta
     5521 ttttaaagtt atcaaattcc tttggaattt ttttgttgaa aatagtatat tctgtcgttt
     5581 ctatatgctg agtttcatac ttatacagaa tatacaataa tattaatact aatactgtca
     5641 ttgaaacctt tataatcata ttctcttcct ctttattcta ttttgttaat attattataa
     5701 tgcttttatg ttttttttaa aacaataaca tcatcacata catataaatc ttttcgacat
     5761 tataaaaata agttatcctt ttctaaaatt ttcatttatt ttagaaaagg ataactataa
     5821 aattatctat aaacctctat attgtgaaat atagtttttc ctttatttaa atttttatat
     5881 atatgctcta aagttccatc aaacctaata gaatgtccag actctaaaat ataagttttc
     5941 ccatccacat taaggtcaag tttaccttct attacgagta catactcttc taaaacataa
     6001 ctatgtgtag atgaaacatg agtacagttt tcctcaagct caattataaa aagttcaaac
     6061 cttctttgag ggctaaaagg gtatattgga tataacttca tgttcccttt ttgctcaaca
     6121 ataggctcta cttcttcttt ctttataacc tcataggtat ttgtattttc atttaatagt
     6181 gtagtaaaat ttattttaaa tccagatgaa attttccata aagtagatac tgtcggactt
     6241 gattcacctc tctcaatctg tccaagcatt gctttactga cacctgtcat ctttgaagct
     6301 ttgtctaaac ttatgcccat ttcttgtctc aataacctta aattttctcc cacattagga
     6361 aatttattat ccatgttaaa atcctcctga tagctatttg tattctatga tttttactcc
     6421 tgtaagttct actatctctt ttaattttaa catatgattg ttacaaattt catcaaattc
     6481 atatatataa tcttcaagcc ctaatgcctt atatttatcg attccaaatt tatgatatgg
     6541 aagtaactcc atcttgcctc ctggaacatt ttgtttgaca aataatgcag tatttcttat
     6601 attttcttca ctatcattta ctccataaat aattggcact cttataacca tagatttatt
     6661 caactttgaa agcctacata tattatctaa tataatttta ttacttactc ctgtatattc
     6721 tttatgaata ttatcatcca tagattttat atctacaaat atgtggtcta ttttctcaaa
     6781 tacatcattt accttattcc aatcaaaata tccacaagtt tcaattgcaa tatttatgcc
     6841 tttatcataa aaaatatcaa ctaattctct taaaaaatca atctgaagtg taggttcacc
     6901 tccagaaaaa gttatccctc cattagattc aaagaaaaat ataaaatcct tttcaacttc
     6961 ctctactatt tcttcaacac tcattttttc ggtcattata caaatggcat ctttaagaca
     7021 taccttgata cactctccac ataaatcaca tttattattt atttgcgttc tatcaaatag
     7081 tgaacttata ttttgaggac aaacatctat acatctatta caggatacac atttatcttt
     7141 cataacacct aattttacta tgttactcca ggaatctgga ttagaacacc acttacattt
     7201 taatttacat ccttctaaaa atatagtagt ccttattcca tctccatcat ttactgaaaa
     7261 atgttgtact tttacaactc tacctttttt actcataatc tacctcataa ttaaaaattg
     7321 ctatgtgaat ttctagctat tatagcatct tgcatttctc ttgataaatt aacaaattga
     7381 gtactatatc cagcaactct cactaataaa tctttatatt cttctggatt ttcttgagct
     7441 tttaatagag tatcatttga tatcgtatta aattgtacat ggaatcctcc taaagcaaaa
     7501 tatgatttaa tcatagatgt tagatttcta agtcctcttt cagtttccac caaatcttga
     7561 ttcagtctta aatttagaag tgttccacca gtatgaacat catgatttat ttttgatgca
     7621 gatttcatgg ctgctagtgg acttgttgta tcagtaccaa caaacggaga aactccctct
     7681 gtcaaaggtt ttgtcgcttt tcttccacaa ggagttgcac ctacgatttt tccagttggt
     7741 acatagtttg atatacccat aaatgcacta ttaaatttag agccaaaaat atccttgtat
     7801 tttctagttt cagtataata aaaatcagaa acttcaattg ctaatgaatc tacataatca
     7861 ttatcatttc catacttagg aacatccaac gcaagttttc ttaattcttc ataaccttcc
     7921 caatcattat ttagtgcttt tgttaattct tctagcgtag tcaccttatc ctcaaatact
     7981 aattttttta tagctgccaa tgaatttgaa actacaccta accctatacc agttaataca
     8041 ggacctatat tatattttgc tcctccttta cttaaatcaa ctcccttatc caaacatcca
     8101 tctacacaag tagataaaaa tggtcttggt accatttctt tatgaatttg ttgagcaaca
     8161 gtagttccta taacagaact ttttacaaga tatgctaatt gctttaaaaa ctcatcctta
     8221 agttcttcaa aagaagtaaa ttctgtgata ttttttgtat ctaaccccat tttttctcca
     8281 gttaatctac tcttaccttc attcatagca tattctaaag ctgctgcaaa attgatattt
     8341 acggctgaag tccactgtcc tgtttttcta aaatgaggaa ctacacaacc acagttactc
     8401 caatctctag catcttctgc atcatatcca tcttgtaata acatttgtgc ccctgcacta
     8461 tcgctgtgta tagcagggaa accagttcct tgttttacca atttagcaac tgccattacg
     8521 aaattatctg gtgctccttg actcactcta acacttaatc ctggctggtg agtttttaca
     8581 ctttctgtag cctttaaaca catatatgag atatcattcg ttccatcagt accatttctc
     8641 tttttaccac caacagtaag attttggaat tgattatatc ctgcaaagta atttgctgta
     8701 tttgctgata tagtccatac ccattctgat aacttaatcc aaagtgcttc tatcaattct
     8761 tgagcttctg attcagttat tttaccttct ctagcatcat tttcataata tggatacata
     8821 tattggtcaa atctacctaa atttaatgcc aatggatttt ctgatagaat accacctaat
     8881 tgaacaaacc atacaaattg tattgcttca taaaaattag ttggagggtt tgctggtaca
     8941 ttcatacata tctctgaaat ttttataagt tctaactttc tcttttcatc tgtttcttgc
     9001 ttactcatct cttttgcaag atttgaatat ctttgtgcca atgtcataat accctctgca
     9061 actatagaaa cagatttgta aaaatctatc ttttcaatat tttctgaaac tgatatatct
     9121 aattctttaa tttttttatc tgcatcttcc ttaatccctt tatatccttt tacaaacaat
     9181 atatcttgat aatctggtgt tacttcacca actgcctgtc tccacttaga atcattatct
     9241 attattcctg tatctactaa tatcttagct gtatcttctg ggattcttgc taagaaaact
     9301 tcttccaaag attttccctt ccaatatgga aatatttctt ttcttataaa ttctatttgt
     9361 tcttttgata ttacatatgg gtcttgagtt cttttatcaa aagtatccat ttctttgtct
     9421 acccattgcc aagaaaactc tggtgtcaat atccctgttc ttctaaattt tcctgctgta
     9481 cctactatta attcattttc aaagattttt ataggaagtt ctttacatgt ctccaaaaaa
     9541 gctttagctc ttcttataca gatagcttct ccctcagttt ttttatgtga ttctgtaaaa
     9601 attctagccc tttcacaaca tatcataggt ttagaattaa tatattcttg tctaaatgct
     9661 tctattctat tagtttgact catttaaaat ccccctttgg ataatatata ctacattttt
     9721 gtatgttata acatacttta attatattat actgtccttt agcataaaat ggaagcaaaa
     9781 taactttttt tttcatctac cttgaacacc tatatattaa tacttttttt gttaaattta
     9841 taaaatctaa aaaaattttc tatgatatat tttctatatt gttatagtta tttctaatat
     9901 actaatgatt tgaaatatca aatttgtata ctagacttag ttttaaaacc ttagttgatt
     9961 gtttaacttt gttaacttca acatgtatat ccttcatata aggaagctcc acaacaccta
    10021 ctcccttatt aaattcttct tcaaccatag aaaaaatttc tttctcagtt aagttcgtat
    10081 tagtcaatga tttaaataaa ttgtatataa atttaatata tttatcactc tcagataact
    10141 tatcatcttc tggatactct ttagcacaga gaatatctat tgttactttt ccttctttat
    10201 cgtcttcaga catagaatag aataaacttc gaatattttt ttcaggtggt ttttcagcac
    10261 catttataga tactacaaag ctaccatctg attttgagtc agtaatacta taaccactgt
    10321 ctattatagc tttcgaaaat ctttgagata aatctttttt tacttcttct ctacctttcg
    10381 catcattaat cttttgtgag taattattaa atagctcgat agattcatct tttttatctt
    10441 caacttgttc tttcaatgaa tctttactaa tttgttctga gctacttaag ttcttatcag
    10501 atacaggaga catacatcct gccatcaatg ctaagctaag atttaaagcc acaattattg
    10561 ccactctatt tatattcctt tttactttca tacacaatcc tcccatcaca ctaaccgctc
    10621 taaagtatta ttcattgcat tagaatatat ttatattttc tttatattaa agatttatta
    10681 ttcaatacat atcaccttcc tatccaattc ttataactta ctattagtaa aattataagc
    10741 tccttaatac agaaataagg atgttatctt ttgttattaa aagataacat ccttattttt
    10801 ttgtatcaat acatatgtcc caactggtaa cttaatcgcc ccaactgtca gtatcttgct
    10861 ccaatggtat atatatattt agtataaatt tatcgatact attttcaaat aataattctc
    10921 cattatattt ttgtaaagaa gatttaatac tctgaatacc tattccatgt acgaacttat
    10981 ccattttgtt tgttaacaat atatttttat taaaagtaag ctgatttagt ttactatttt
    11041 cacatttaag tacaaaaaat gactttgtta tagttccttt aattctaata tatttattaa
    11101 tattttcatc cactatttta tcacatgctt ctattgcatt atccaaaata ttagaaaata
    11161 tgcttgacac atctataggt tttacaaaat ttacttttga aaaatttata gagcaagtaa
    11221 atttaattcc tttttttaaa catatttcac tcttttcgtt gattataatg tctaatatca
    11281 tatttcctgt attatagata tatttaaatg tttttatttc atcttttaaa ttgttaacat
    11341 attcattaat ttctttgcta ttgtttctaa ggttgtctat acaataaatg tggttattta
    11401 tatcgtgata tagtttctta actcgcatat gagattcttg aatacttaaa taatgcgcat
    11461 attgcatatc taacttattt tttattaatt cagcttgaga ttttaatcta ttctctttta
    11521 ttatttcttt aaacaataat ataagaaata tactactagc tcctaccaac tttggattaa
    11581 caaaattatc aaaatagtat ccttctgaat gtaatgaccc aaaccgttct aattttttta
    11641 atacataatt tacaagcata ttcattgtat tagctaataa ggcaaataat aaatatacat
    11701 aatttctctt atcttggcta attaatttta tagacttcct aaaaaagcta aatacaagat
    11761 atataagaat aattattaaa ttactatata tctgaatttt tatagattct aagtgaggta
    11821 ctaataagga ttctttagat gaaataacat tacctgtaat tgcaaaaaat agtattctat
    11881 atataacagc atccaaacat ttataatata cactaaatag ataaaagaaa atggaatact
    11941 taatataatc catcttataa aaataattat agtatcctcc ccaagcaata aatgctaata
    12001 tataaaataa atagtttata ttactaaagt aacttcctcc attaaataat ctacttatct
    12061 gaattataat ataaataaga aacaagacaa tatagaatac tattttttca ttttttttac
    12121 tatccttttt ataaattaaa attccgttca ttatagaaaa ctgtataaat atagataaaa
    12181 ctactaatag ctcattcata aaatttagca tattgtatca ccaagaaatt ttaaaaattt
    12241 ctccttaact tctttatatc tatatctact aataggtacc tcttctccac tttccagtat
    12301 tgcagcatta ggctttatat tttcaacata tcttaaattt actataaaac ttttatggca
    12361 tcttataaat ttatatggat ttaattcttt ttctatttta cctaaactat attttatatc
    12421 aaaattcttg tttattgtgt gtattagcat atcttttttt tgaacttcta tatattttat
    12481 ttcatttaaa taaatcttat atgtattaga cttatttttt attgtaatat gactttcctt
    12541 atttatttca atatctttga tacaagttag cacatgtttt tttagttctt ccaactctat
    12601 tggctttagt aaatacctat aagctcttac ctcataacct tcttgcacat aatctattaa
    12661 tgaagttaca aatattattt ccatttcatc gtctacttct ctaatctttc ttgcagtatc
    12721 cattccattt aatttttcca tttttatatc aagaagtaat atatctatat cttttaaatt
    12781 actttccagt aactcttctc cagatatata cttttgtatc tcatattcta tacttatatc
    12841 ttttaatata ttctcaaggt aatcttctat taaaagttgt gtttcttttt catcttcaca
    12901 aactgctatc ttaatcataa aatgcctcct
//
LOCUS       DAELSY010000110        12917 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.110, whole genome shotgun sequence.
ACCESSION   DAELSY010000110 DAELSY010000000
VERSION     DAELSY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12917
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.110"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            164..580
                     /locus_tag="KPG09_002754"
     CDS             164..580
                     /locus_tag="KPG09_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967350.1"
                     /translation="MSKSETGVKSKLILELLVITYLFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSKFILSKVNQIAITIMMYSIVIIAIFFATPYISNI
                     NISNLDTGIILIAVLFIQSLSKLILYIYFDKRGICN"
     gene            581..793
                     /locus_tag="KPG09_002755"
     CDS             581..793
                     /locus_tag="KPG09_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0967351.1"
                     /translation="MAISMKTKIKEYREKLLMTQNELAKSVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFGTTVENLFEFIEED"
     gene            1428..2471
                     /locus_tag="KPG09_002756"
     CDS             1428..2471
                     /locus_tag="KPG09_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBG0967352.1"
                     /translation="MKRKIAVVLLTVAMLLSLVACSNDNTSKSDKLEDTLVVYTTHGE
                     DMLGAISEEFTKETGVNVEFINLKGELADRVRSEKDNPQADIMFGGDTSTYMQLKEEG
                     LYEKTEPSWNNELDKSYKDSDGYWYGTIKTPVMMFYNTKLLSADKAPKDWSDLTKEEY
                     KDLIVTRDSLSSSMRSTISNLINYYSNTKSEEEAWKYLEDLNKNIKNYYNSGSVMYSA
                     IGKGEATISVAVLSDIIENKEKNNMPLEVVDAESGSVVITDCVAALKNAPHPNAAAKF
                     VDFVGSAKVQAMVANDFNRIPTLDSALKDSPKWMSKEYKAMDVDWSVIGKNQSNWIEK
                     WETDIIDNNKTIK"
     gene            2732..3606
                     /locus_tag="KPG09_002757"
                     /pseudo
     CDS             2732..3606
                     /locus_tag="KPG09_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3625..4617
                     /locus_tag="KPG09_002758"
     CDS             3625..4617
                     /locus_tag="KPG09_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967353.1"
                     /translation="MKNIELKEINHYYGKEKVLHDINISIKEGEFFTLLGPSGCGKTT
                     ILRIIGGFIKPSSGSIYVGDKNITNLEPENRNMGTVFQNYALFPNMTVEENVSYGLKI
                     KKLSKKIIKEKCDNYLELSGMKDFRKKKIDELSGGQQQRVAVARSLATEPTMLLLDEP
                     MSNLDIALRIKMREEIREIQQKTGITTLFITHDQQEALAISDRIAVMDKGKVLQIGVP
                     IEVYKSPLNDFVANFVGTSNCIGKEDYVNFNIEKETKPYIYKRPEEMILLQNTNQSGF
                     VKVKVESKNFLGAILEYTVSNDGKKYQVTELNRLNNSNKLNVGDMAYLGVLQGD"
     gene            4619..6313
                     /locus_tag="KPG09_002759"
     CDS             4619..6313
                     /locus_tag="KPG09_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG0967354.1"
                     /translation="MIKKINRSKFTSKNVIFIVLYSILFWILIGYIIIPIGNTFVQAF
                     QGKGGYSFEVFKEYFSNTNNLRVVSNTIILGLGSVFVCGIMGISLALYMTFVCDKHKK
                     IIHILLLSPMMIPGVIMVIACIQIYGESGIVTKSIEILLPFIKKPYDFSGLKGILFVV
                     AYTQYVYFYLNVYVALKYVDYSTVEAAKGMGASKLRVFFDVIWPVITPAVITSTIITF
                     ASGISSFSAPNLIGGGFKVLSTQIVRSKANNHMDIASVQVITLLLMGILFMILLQYYG
                     KKYGVVPSLKSQSYKVHNRKKDLFTFVCKVIIEIQIIMIMLPIVTIVYLSFVSTHSIM
                     TTIFPRDFTLENYQMIFQKSRVLQPILNSFKMSLMAVAVGLIITVPVSYLVIKKDTAY
                     NRLAKFIIMLPWSMPASAIAVNLINTFNQKSIFAFNESLIGGFYIVPIAYTIYALPLL
                     FSSNEVAMKSINLGLEESSRSLGAGRIKTFYNVIVPNMMPGIISGAILVFIRTIGEYT
                     MSALLYGVYNRPISISIVTNMQEFKIGVSMAYGVLVIGICYIALGIIFKLDRKRFT"
     gene            6864..7742
                     /locus_tag="KPG09_002760"
     CDS             6864..7742
                     /locus_tag="KPG09_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBG0967355.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQGANEKNAPV
                     ILAIHPNELSFLGDSFVVSCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            7778..9673
                     /locus_tag="KPG09_002761"
     CDS             7778..9673
                     /locus_tag="KPG09_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0967356.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSETKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFPPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVAKPTIRSENS
                     EVELLGA"
     gene            9941..11848
                     /locus_tag="KPG09_002762"
     CDS             9941..11848
                     /locus_tag="KPG09_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0967357.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDILSESEKVSSIMIENMSQFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            11838..12287
                     /locus_tag="KPG09_002763"
     CDS             11838..12287
                     /locus_tag="KPG09_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0967358.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNISERLLNVHTKKEILDIFYEV"
     gene            12387..>12917
                     /locus_tag="KPG09_002764"
     CDS             12387..>12917
                     /locus_tag="KPG09_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HBG0967359.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINS"
BASE COUNT         5177 a         1339 c         2252 g         4149 t
ORIGIN      
        1 atattatttg taaccattag atgtctatta tgaaatcttt tatggactat atttgtaaaa
       61 atattattaa gtatttacaa tattttcttg caatttattt ggaaaggtta tatatttata
      121 acatgaagtg ttataaatat ataaccttta aggtggattt tatatgtcta aatcagaaac
      181 tggtgtaaaa tcaaaactca tattggaatt acttgtaata acttatcttt ttatagctgt
      241 attagctact aagtatattt ttaaaggtcc aagtactgca tctcttccag gactgagctg
      301 gttaatagtc tttggatgct attcaattta taataaaaaa tataatgatt tagttgatga
      361 atcatctaaa tttatactat caaaggttaa tcagattgca ataacaataa tgatgtattc
      421 tatagttata atagctattt tctttgcaac accttatata agtaatataa atatatcaaa
      481 cttggatact ggtataattc ttattgcagt tttatttata caatcgttat ctaaattaat
      541 tttgtatata tactttgata aaagggggat atgtaactaa gtggcaatat cgatgaaaac
      601 aaaaattaaa gaatatagag aaaaactcct tatgacacaa aacgaacttg ctaaatcagt
      661 tggggtaaga cgtgagacta ttgtacactt agaaaatgga aaatataatc cttctttaaa
      721 gttagcgatg gatattgcta aagtattcgg gaccacagta gaaaatttgt ttgaatttat
      781 tgaagaagat tagtagatag gatataaata tttataagat tgttaataaa atatagttat
      841 ttaattatta attttgttaa aaatatttat aaataatata atgtatgagt aaagttaggt
      901 tgtaattatt ttaagataag aagactactg taaatgataa aaattagtga atatacagcc
      961 aattaataaa taatagataa aactttaaag cttagatgtg aggatgagat atggtcttac
     1021 agtagttcac acttttaatt aagacttaaa atggaaacca tacacataca tacactattt
     1081 actgaaagtg gactagataa aaaaataata agtgatttaa gaaagtatct catatactat
     1141 ataagtatta ttttaggtga cctatttata ggtcattttt tatgtatata aatgataata
     1201 atactaatat ttaaaatagg ttttaaagaa ataaaatatc cagattgaaa aatggattaa
     1261 tgttaataaa aaaattatat gtataaaaat aggtataatt atagaaaaaa acaataataa
     1321 aataagctgt tatagaaaaa tacaattata tagattgttg ttgaatcatt gactgcaaga
     1381 ataataacat ttaaaatgaa catggagaat atgggggagg aatcaatatg aagagaaaaa
     1441 tagcagttgt tttattaact gtagcaatgt tattatcact tgtagcttgt agcaatgata
     1501 acacatcaaa aagtgataaa ttagaggata cattagtagt atatactaca catggagagg
     1561 atatgctagg agcaatttct gaagaattta caaaggaaac aggagttaat gttgaattta
     1621 taaacttaaa aggtgaacta gcagaccgtg ttagaagtga aaaagataat ccgcaagcag
     1681 atataatgtt tggtggagat acatctacat acatgcaatt aaaagaagaa ggattatatg
     1741 aaaagactga accaagctgg aataatgaat tagacaagtc ttacaaagat tcagatggat
     1801 attggtatgg aactataaaa acaccagtaa tgatgtttta taacacaaaa ctattatcag
     1861 ctgataaagc gccaaaagat tggagtgatt taacaaaaga agaatacaaa gacttaatag
     1921 ttactagaga ttcattatca tcatctatgc gttctactat tagtaactta atcaattact
     1981 actcaaatac taagagtgaa gaagaagctt ggaagtatct tgaggattta aataagaata
     2041 taaagaatta ctacaatagt ggaagtgtca tgtattcagc aataggtaaa ggtgaagcta
     2101 caatatctgt tgcagtatta agtgatataa tagagaataa agaaaaaaat aatatgccat
     2161 tagaagttgt agatgcagag agcggttctg tagtaatcac agactgtgta gcagctctaa
     2221 aaaatgcacc acatccaaat gcagcagcta aatttgtaga ttttgttgga agtgctaagg
     2281 ttcaagctat ggttgcaaat gattttaatc gtattccaac attagattct gcacttaaag
     2341 attcaccaaa gtggatgtca aaagaatata aagctatgga tgtagattgg tctgtaattg
     2401 gaaaaaatca gtctaactgg attgaaaagt gggaaacaga tataatagat aacaataaaa
     2461 ctataaaata gcctaattaa ataatttaat attatataca aaaatagtga ggattttcct
     2521 cgctatttta cgtataagga aactatagta tctcataatt ttgtataatt catacaacaa
     2581 aatgatacta atacatactt attaaataaa atagtttttg agtaactgat gaagtaattg
     2641 actatataaa gtgatttgta gacatagtct ctcaaataaa gtgaattttt tattatgcaa
     2701 aacttttaat tatagtaaaa gtacaattta tatgggcaag gagataaaaa tggaactaaa
     2761 aattagtgat tataaattat tatgtaataa aggtagtggc gcttttaacg aagatatcgt
     2821 aggtttatca ccttatgggg catgggtact tgatggagct acaggtttaa ataataaaaa
     2881 tttagtctct acagaaagtg atgccaaatg gtatgtaagt tggtggaata gttatcttca
     2941 tgagaatatg gaaagagaag atagcttaaa agtaattatt aaagaaggca ttgaggagat
     3001 aacaaaagag tactacaaat taacagatga taaagaagta gaagcaatag atattccttc
     3061 ttgttcatgt tctatattaa agttttataa agataagata gagtatttac ttttaggaga
     3121 ttgtacttta ttagttaaca ataatgaatc tgtgaaaatt ttaaaagatg agactttatg
     3181 tgaacttgat aaaaaagtat ttgattttat gaaagaagcc aaagatgcta caaaccttac
     3241 tttaaaggag agaaaagccg aggtaatgcg attataatag agaatagact taagaaaaat
     3301 actgtggggg gatattggac tttggaattt tctaaagagg ccgttgacaa ttgtgtaagt
     3361 ggatttattg atatagaata taaaatgaaa attatgatgg ctagtgatgg tttttcatgt
     3421 ggatatgata gatataagat ttttgataaa aatgaaatga tgaaaatagc agagattaaa
     3481 ggtatagatt atatatatag aaaggttagg gaactagaaa aggatgatga aatggcaata
     3541 gagtttccta gatttaaaat ctatgatgat tcatcatgtg tatatttaga gatttataaa
     3601 tattagataa tttatggaga cgatatgaag aatatagaat taaaagaaat taatcattat
     3661 tatggaaaag aaaaagtatt acatgatatt aatatatcta ttaaggaagg agagtttttt
     3721 actttactag gtccttctgg atgtggaaaa acgactattt taagaataat tggtggattt
     3781 ataaaacctt cttctggaag tatttatgtt ggtgataaaa acattaccaa tttagaacca
     3841 gaaaatagaa atatgggaac tgtttttcaa aactatgcac tgtttccaaa tatgacagtt
     3901 gaagaaaatg tatcttatgg attgaaaata aaaaagctgt ctaaaaaaat tataaaggaa
     3961 aaatgtgata attatttaga gctgtcagga atgaaagatt ttaggaaaaa aaagattgat
     4021 gaattatctg gtggacagca acaaagggtg gcagtagcaa ggtcattagc gactgagcct
     4081 actatgcttt tattagatga gccaatgtct aatctagata tagctttaag aatcaagatg
     4141 agagaagaaa taagagagat acagcaaaag acaggtatta ctactttatt tattacccat
     4201 gatcaacaag aagccttagc tatttctgat agaatcgctg taatggataa gggtaaagtt
     4261 cttcaaattg gtgtgccaat agaagtttac aaaagcccat tgaatgattt tgtagcaaac
     4321 tttgtaggta cttcaaactg tattggaaaa gaagattatg ttaattttaa tattgaaaaa
     4381 gagacaaagc cttatattta taaaagacct gaagaaatga tattattgca aaatacaaat
     4441 caaagtggtt ttgtaaaagt aaaagtagaa tctaagaatt ttctaggagc aattttagaa
     4501 tatacagtat caaatgatgg gaaaaaatat caagtaactg agcttaatag actgaataat
     4561 agcaataaat taaacgtagg ggatatggca tatttaggtg tgctacaagg tgactaatat
     4621 gattaaaaaa ataaaccgta gtaaatttac atctaaaaat gttattttta ttgttttata
     4681 cagtatatta ttttggattt tgattggata tattatcata ccaataggca atacttttgt
     4741 acaagcattc caaggtaaag gtggatattc ttttgaagtt tttaaagaat atttttcaaa
     4801 tactaataat ttaagggtag taagcaatac tataattttg ggattgggtt ctgtgttcgt
     4861 atgtggaatt atgggaatat cacttgcctt atatatgaca tttgtttgtg ataaacataa
     4921 gaaaatcata catattcttt tactaagtcc aatgatgatt cctggagtaa ttatggtaat
     4981 tgcatgtatt cagatttatg gagaaagtgg aatagttaca aaatctatag aaattttact
     5041 cccctttatc aaaaaacctt atgattttag tggattaaag ggaattttat ttgtggttgc
     5101 ttatactcag tatgtatatt tttatttaaa tgtatatgtt gccttaaaat atgtggatta
     5161 ctcaacagtt gaagctgcaa aaggaatggg agcatcaaag ctaagagtat tttttgatgt
     5221 aatatggcct gttattacac ctgctgttat aacttctact attataactt ttgcatcagg
     5281 gattagttct ttctcagctc caaatcttat aggtggtggt tttaaagttt taagcactca
     5341 aattgttagg tctaaggcta ataaccatat ggatatagct tcagttcaag taattacttt
     5401 attactaatg gggattttat ttatgatttt attacagtat tatggcaaaa aatatggagt
     5461 agtgccatcc ttaaaatctc aaagttataa agttcataat agaaaaaaag acttatttac
     5521 atttgtatgt aaggttatta ttgaaataca aattattatg attatgcttc ctatagttac
     5581 tattgtgtat ttatcctttg taagcacaca ttcaattatg acaaccatat ttcctagaga
     5641 ctttacactg gaaaactatc agatgatttt ccaaaaatca cgtgtattac agcctatatt
     5701 aaatagtttc aaaatgtctt taatggcagt agcagtagga ttgataatta ctgtaccagt
     5761 atcatattta gtaattaaaa aggatactgc atataatcga ctagcaaaat ttattataat
     5821 gttaccttgg agtatgcctg caagcgccat tgcagttaac ctaattaata catttaatca
     5881 aaaaagtata tttgctttta atgaatcctt aataggaggt ttttatattg tacctatagc
     5941 atatacaatt tatgctctac cattattatt ttcatccaat gaggttgcta tgaagtcaat
     6001 aaatttagga ctagaagaat catcaaggag tttaggtgct ggaaggataa aaacctttta
     6061 taatgtaata gttccaaata tgatgccagg aataatttca ggagctatat tagtattcat
     6121 tcgtacaatt ggggaatata caatgtcagc cttgctttat ggtgtttata atagacctat
     6181 ttcaatttct attgtaacta atatgcaaga atttaagata ggagtatcta tggcttatgg
     6241 tgtattagta attggtatat gctatatagc attaggaata atttttaaat tagatagaaa
     6301 aagatttaca taagataaca aaggctaatt tataaattgg tgcttagaaa accaaatcac
     6361 tttattagct aaagatgaat ttgaagggtg acactttggt gttttatgta aagtttatgg
     6421 aagtaaaaca ttaaataatc gtgagtatga ttcctataaa tcgtatatac tattgaaatc
     6481 caagaaaagg tattggctaa atagctaata tctttttaat tttttgactc ccatatagca
     6541 ttaactatta catgagtatc tttaaattta tagttaaagt atcttaattg tttattataa
     6601 aaatatagga tggctaaagc acattaaatt aaaacactta aaaagagaag atgtacatga
     6661 gaaatataat taactatgat acagacttct aaaaattatt tagtgaaaag gaacttagag
     6721 tgtataataa aagttccttt tcaataagga aaacgattgt tattttctga gcttcattac
     6781 cctgatatta tttaaatata aacaacaaca aaattaacaa gaagtaaatt caacctaaaa
     6841 tttaaaatat cgggaggtac aatatgttaa tcaatatgaa ggaaatgcta aaagtggcac
     6901 aagaaaatca atttgctgta ccagcattta atataggaag tgggcaaata cttaaagcag
     6961 tagtacaggg tgctaacgaa aaaaatgcac cagtaatact tgcaatacac ccaaatgagc
     7021 taagttttct aggcgatagc tttgtagtta gttgcataga agaagcaaat aagagtaaag
     7081 tacctatggt aatacactta gatcatgggg aaaataaaga acaaatatta agggcaataa
     7141 gatgtggatt tacatcagta atgattgatg gctcacattt accatatgaa gagaatgtag
     7201 ctatatctag ggaaatagta gaaatagcaa aaggattaaa tgtgtccgta gaaggagaac
     7261 ttggaacaat aggaacaaca ggaacatcaa gtgaaggtgg tactgatgaa ataatatata
     7321 cagattcaaa attagctaag gattttgttg aaaaaacagg agtagacaca ctagctattg
     7381 caattggtac agcacatggt atatatccaa aaggttttaa accagagctt aaactagatt
     7441 tattaaaaga aataagagaa gtagtagata tacctttagt tcttcatggt ggttcatcaa
     7501 atccagatga agaaatagca caagcagtta aactaggagt ttgtaaaata aatatttcga
     7561 gtgatgttaa gtcggcatat tataagaagt gcagagaact attagaacaa aacccaagct
     7621 tatatgagcc agatactata tatccaccat gtataaaagc tgcaagagaa gttatagagt
     7681 ttaaaatgaa tctattcaat gctatagata agttaaagtg cttttataaa aataaaaatt
     7741 aattatgagg taacaaatta aaaggagggg agaatttatg ataggtaaaa tactacgaag
     7801 cgattgtatt gtactagata aacaattaca aacaaaaaaa gatgtaatta attatatggc
     7861 aaatcatttg taccatggaa attatataaa taataaagaa aagtttatag ctgatattaa
     7921 taaacgtgaa aaagaatcat caactaatat gggtaattta atagctatac ctcatgctaa
     7981 gtcaaatgta gtaaatcaac ctgtaatgat gtatttaaga acagatagag agattacaga
     8041 taatgatggt caaaaactta ggtcattttt tatgatagca gttccagaat caggttcaga
     8101 tgaacatctt aagattatat ctaaactagc tacatatcta atggaagaaa attttcttaa
     8161 gcatctacta agtgcagaaa ctccatacga aatattaagt atatttaata ataaagctgc
     8221 agaatcagga gattttagta ataatcaaaa taaaggtctt atagttggga taacaggatg
     8281 tccaacaggt atagctcata cttttatggc aaaaaaagca ttagaggaag cagggcaaga
     8341 acttggatat gaagttaaaa tagaaactca tggagctata ggtgtagaaa ataagttgac
     8401 taaagaagat ataaaaagag ctgaatacgt aataatagct tgtgataaag aagttcaact
     8461 gagtagattt ataggaaaga aagtgctaca aaagaaagta gtagatgcta agaatgtttc
     8521 attagctaaa aatttaatta taaacgctga aaatggaatg gctaaagaaa ttaaaccagg
     8581 tggaaagact ttagaagatt caaaagaaat gcttcaaggg tcagagacaa agggtgttta
     8641 taagcactta atgagtggtg tatctgaaat gattccattt gtagtgatag gtggaattgg
     8701 tatagccata gcatttatgt ttggtatatt tgcatcagac ccaactcatg aaacatacaa
     8761 ccctatagct ggattctttg gacaacttgg aggcgattca gcatttaagt tatttatacc
     8821 gatacttgct ggttttatag ctaaatcaat agcaggaaga caaggatttc ctcctgctat
     8881 gatagctggt tttatggcag ttattggagg ttcagggttt ataggtggta tggtagctgg
     8941 ttttgcaaca ggatatatag ctttaggagt aatgaagata acagaaaatt taccagatgt
     9001 attaaaagga gtaaatgctg tattgatatg tccacttttg attgcattta cgagtggagc
     9061 tataatgttc tttgtagtta atacgccagc atcatggtta aatgaagctt tacaatcttg
     9121 gttacaaagt atgtctggaa ctaataaagt attattaggt gcaatattag cagggatgat
     9181 ggcatcagat atgggaggac ctattaataa aacagcttca gcttttggat tggctatgtt
     9241 ttcaagtaat atatttgagc catcagcagc attaatggta ggaggaatgg tacctccatt
     9301 agcgatatca ttagctatgt taatattcaa aaataagttt actaaagaag aaagaaagtc
     9361 atctctacca actgctataa tgggggcatc tttcataaca gaaggcgcaa taccgtttgc
     9421 agctgcagac ccattaagaa tgattccagc aaatataatt ggttcttgta taggtggggc
     9481 aatctgtatg agtttagata taactttaat ggcaccacat gggggaatat ttgtaatacc
     9541 atttgcatgt agtgcaccag ttgcatatat agcttgtata ttgattggta cagtaataac
     9601 agcattgatt ataggggtag caaagcctac tataagaagt gaaaacagtg aagttgaatt
     9661 acttggtgct taaattataa ataatagtaa aaataagtaa tggaataaca acaagtatat
     9721 agaataataa taaggatatt tagaatcagt taactacttc taaatatcct taatttttat
     9781 tttgaaatta tattatctat tatatcaatt tagtaaggtg tatcataaat tcttttatga
     9841 tacagaagta tcttagtaat tttacatagt tagtaaatat gttaaaatag tacttaatat
     9901 aaacatagaa atatataaac ataggtaggg agggctaagt atgcttgtgt cgaggcaaat
     9961 tgaatttctt aaattgatac taaacgaaaa taaatataga cctataaaat actttaaaga
    10021 taaattaaat gtatctgata aaactttaca aaaagatttg aagatgattg agaaatattt
    10081 agaaactttt aatataaaaa ttgatataaa aagaggctat ggaatattaa tagaagattt
    10141 agcaaagaag aacatagact taattaatag tttaaatata caatcgaagg aaaataaatc
    10201 tttatcagta gagcagagaa gaatggagat tttaaagtat ttattactaa attcaaataa
    10261 tataacttca ataaatcagt tagcagataa atattatgta agtaaaactt ctatagtaaa
    10321 tgattttaaa tatatagagg catggataaa agaatacaat ttaaaactta ataaaacttt
    10381 agaaggaact aagattatag gtaaagaagt ggatataagg aaatcgattg ccaaaatgat
    10441 ggatgattta ttagatgaaa attatgaaca acatgatata tctgagttaa caagattaga
    10501 ttctgctaca tttagtgcat tgataaattt atttgatatt gattctatta tatttgtaga
    10561 aactataatt acagaattag aatacaattt aggatatact ataaatcagc cctactatat
    10621 aaatttaatt acccatatac ttatatgttt aaagagaatc gaagaaggta atcaaataga
    10681 atctcaggaa gaaagagaaa tttctgtaga taatttggac gagttagtat ataaaaatgt
    10741 cactacactt atagataaaa tagaaaatag atacagagta aaaataacga aagatgaaat
    10801 tatgtacata tatatttttt tggtatcatc tggatttagt agcgaaaata atagtagtta
    10861 taatgatgaa tcagatatat taagtgaaag tgaaaaagtt tcatctataa tgatagagaa
    10921 tatgtcacaa tttttaaata taaatctcaa aaatgatgac ttactacaaa aaagtctagc
    10981 atctcatgta aggcctatgt taaacagatt aagatatgat atccaaataa aaaacccgtt
    11041 gttaggagag atacaagagc gattttcaga tgtgctggga ttatgtatga tgactataaa
    11101 tattatgaca gagtatgata atctgaagaa tattagtata gatgaagttt cgtatcttgc
    11161 cacatatttt caagcggcaa tagaaagaaa tatgagtagt aaaagagtaa tagtagtttg
    11221 tcatacagga tatggaacat ctcagctcct tgcagccaga ttaaaaagag aatttcctga
    11281 atggactata gttgatatag ttcctatgca tcagttgagt aagcgaaatt tagatgatgt
    11341 agactttgta atatctactg taaacttaga tttgaaagat aaattacata tagttgtatc
    11401 agtactatta atggaaagtg atattaataa tataaaaaat gctttacttt ctaaacctaa
    11461 aaaaagtatg aacttagata caaacttatt aggaatgtat ttagaagata atatatattt
    11521 taattttgat gtagctcaga tggaaagttt aataggtgtg aatttaggga gtaaatatga
    11581 atctatttca ttaggaaaag atttgaatat atatatacat aagcaaagta aattaaataa
    11641 agggataatg aatatcaata atgttactag aacgattgat atttatttag aaattagtaa
    11701 tcaaagtttt ttaaagaata ttttaatgga aatatcaaat ttatataggc aaaagaacta
    11761 tataaactat ataatagatt gcaagagcaa agaggatatt aaaatattat ttaagaataa
    11821 tctattggag aaaacaaatg gaaattagag aaatattaac agaagaaagc attattttag
    11881 atttgaaatc aactacaaaa gaaggcataa tacaggaatt agtagataaa ttatacgata
    11941 caggaagtat aactttaaaa agtaagttta taaaggcatt aaaacataga gaaaaaatag
    12001 gctctactag tataggttat caaatggcca tgccacatgg aaaaacaaaa tatgtaaaga
    12061 aggcatccat agtaataggt aaatctaaaa aaggtgtaga ttggccaagt atagatggct
    12121 tacctgtaaa ggtaataatt atgtttgcta tgccacaatc tggaggcaat gaagatatga
    12181 tattaatatc taaaatagct tacattttag gtggtaaaaa tataagtgaa agacttttaa
    12241 atgtacatac aaaaaaagaa atattagata tattttatga agtgtaggta ttgtataaat
    12301 tataaatttg aggtttttaa aataaaatgt gtattctttt aaatagtata gattaatttt
    12361 tagacactag agaggagagg atatctatgg caatatattt agaaatgcct aaattaatac
    12421 atgaatttcc ttttcgtagt ctatcaaatg aaggagaagt attaacaaca ccacactggc
    12481 ataaggaaat agaaatatta cttataacag aaggtgtagt aaatctattt ataaatgaca
    12541 agcctatgca attaaaaaaa ggcgagatag tgataataaa aggaggagat attcattata
    12601 tactgccatc accgggaagt gaaagattag tatttcaatt tgatatatct ttttttaatg
    12661 atttaatttt attaaatgag gaaaaagaaa gcttaattaa tttattcagt agtataaaaa
    12721 aatgtagtag agattggtca gcaaaaataa gaaagtcagt tttcgatata ttgatagata
    12781 tctacaatga agacctatac aaaatagaag gatatagtta tgcaataaaa ggtaaaatgt
    12841 ataatttaat tcctatatta tatagacaga ttcctagaga aattgaagaa aatacagtag
    12901 aatatgaaat aaactct
//
LOCUS       DAELSY010000111        12901 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.111, whole genome shotgun sequence.
ACCESSION   DAELSY010000111 DAELSY010000000
VERSION     DAELSY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12901
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.111"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            154..867
                     /locus_tag="KPG09_002765"
     CDS             154..867
                     /locus_tag="KPG09_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBG0967360.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLFLDKIKEDTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            985..1854
                     /locus_tag="KPG09_002766"
     CDS             985..1854
                     /locus_tag="KPG09_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG0967361.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSITSNIITV
                     IPEKSRVEVLQVDGDWSKIVYDDKIGYVFNYFLSTDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(2009..3154)
                     /locus_tag="KPG09_002767"
     CDS             complement(2009..3154)
                     /locus_tag="KPG09_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG0967362.1"
                     /translation="MSTYYVPPINLLGKGCLNEAKDSIKSLGTKKAFVVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVDKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIVAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAVEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKEKIIELFMKIS"
     gene            complement(3487..4263)
                     /locus_tag="KPG09_002768"
     CDS             complement(3487..4263)
                     /locus_tag="KPG09_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG0967363.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            4845..5819
                     /locus_tag="KPG09_002769"
     CDS             4845..5819
                     /locus_tag="KPG09_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG0967364.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            5917..6222
                     /locus_tag="KPG09_002770"
     CDS             5917..6222
                     /locus_tag="KPG09_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG0967365.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGNEISFKLKDKTVKTITALDNI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            6378..7538
                     /locus_tag="KPG09_002771"
     CDS             6378..7538
                     /locus_tag="KPG09_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG0967366.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            7588..8490
                     /locus_tag="KPG09_002772"
     CDS             7588..8490
                     /locus_tag="KPG09_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG0967367.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESIFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            8570..9463
                     /locus_tag="KPG09_002773"
     CDS             8570..9463
                     /locus_tag="KPG09_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBG0967368.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNKLNIC"
     regulatory      9516..9697
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            9925..11055
                     /locus_tag="KPG09_002774"
     CDS             9925..11055
                     /locus_tag="KPG09_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0967369.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            11127..11972
                     /locus_tag="KPG09_002775"
     CDS             11127..11972
                     /locus_tag="KPG09_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG0967370.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KLIKK"
     gene            12594..12701
                     /locus_tag="KPG09_002776"
     CDS             12594..12701
                     /locus_tag="KPG09_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0967371.1"
                     /translation="MGRHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            12703..>12901
                     /locus_tag="KPG09_002777"
     CDS             12703..>12901
                     /locus_tag="KPG09_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0967372.1"
                     /translation="MVVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNNKETFTYKQCSSDLNNLKKE"
BASE COUNT         4887 a         1484 c         2311 g         4219 t
ORIGIN      
        1 gtattattaa gtgaggttaa gcgttataat taattataaa tattgaataa tctaaatgga
       61 taagataatg gtattaaaaa ttatcttaaa aggtgaaata atctgtattt aatgtagtac
      121 agttatatct ttaaagcaag gtggtgaaat gatataaata agatatctaa aagagggttt
      181 gatgatagtg ataaaagatg gttttcaagt aaagaattaa caagacttat aaaggctaaa
      241 gaagaaatag aatggttatt aaatagggat tacaaaatag acccagtcgt tacttttgtt
      301 ggtgatagat atcagttttc tataagacaa agagatgcat taaaaagagc agtctgtact
      361 gatgaaaaaa atattataag acaatcaaaa aggctttttc tagataaaat caaagaagat
      421 accgtaaata tagatggctt taatttgata atatccatag aggtagcatt atctggtggt
      481 actctagtaa taggaagtga tggaaatata agagatttag caggtcttag gggaacctat
      541 aagattatag ataaaacaga agaagctttg aatctgatag gtaaattttt taataaatac
      601 aatgcacaga atcttaaatt ttatttagat gctccagttt ctaattcagg aaatttaaag
      661 tataggatat tagagcatgc caaaacttgg ggaattgaaa ctgaagtaga attagtaaaa
      721 aatgcagatg ttgtattgga aaaattagat agagtagtta gtagtgatgc agtcatagtt
      781 gacaagtgta taagttattt taatgtggct agaggtataa ttgaagagta tattaaagaa
      841 tgtaatatca taaatttaaa taaatagata aatagaggtg agaatatata agtatatgta
      901 gcaatattat tagtaataca agtaaagttt gaaaatagga gaacgaatac atatcgaatt
      961 tcataaaatg ttgtaaaaag acatatgaga ggatggatat gtgtgaaatt aactaaacta
     1021 aatattaaaa aatatagagc acctatatat aaatatgctt tagcaaatgt aaatttgagg
     1081 tctgctaaat ctactaattc aagtatcata acagtaatac ctcagggagc taaaatggaa
     1141 gtattggatg aagaagatga ctggattaag gtgatgtaca attctcaaga aggatatgtg
     1201 tataaggatt tggtatcagt aagcgaatat gcttggagta acttaaactt aagagaagat
     1261 aaatctataa cttccaatat aattacagta attcctgaaa aatctagggt tgaagtcctt
     1321 caagtagatg gagattggag taagattgta tatgatgata aaataggata tgtttttaat
     1381 tattttttat caactgatgg gaataaacca aatgaattag attataagta tttttatact
     1441 gacatgatta aatttgttaa cgaaaataat attaaaagta ctagcgatta tctaattgta
     1501 acagacctta gaaataaata tacttatatt tttaagaaag ataatggagg ctgggggcaa
     1561 ctttacaagt ggcaatgtac tataggcaag cctgagactc caactataac aggtatattt
     1621 tatataagtg gaagaaaacc ttcttttgga acggatgagt attcagttaa gtatgctact
     1681 agaattaaag gtggatatta ctatcattct gtgctatatg attcaacagg aagttatata
     1741 atagatggca gacttggtga agctcttagt catggatgta taagattaag tacggaaaat
     1801 gcaaagtgga tatatgataa tataccagat acaacaacag ttataattca ttaagaaaaa
     1861 ttttgagttt ttattgggat aatgattctc aaattaatag gattatatat taacttcttc
     1921 ataaagagta ctatttaaaa agtgtaataa gagagtgcta ttaacaaaac aaagatattt
     1981 tgaggcactc tcttataaat atacataatt aagatatttt cataaacaat tctataatct
     2041 tctccttatc aaagaaaaca ggattagcac cagcacaagc atctttcata gagttttctg
     2101 caagcaattc aaaattagga ttatcaacac ctacatcttt taaagatttt gggattccaa
     2161 cctcttcaga aagagctttt atcttatcta ttacaaagtc aacacactct ttatctgatt
     2221 tattatttac atccattcca ataacttcag ctattggtcg gaatttggca ggggctgatt
     2281 tagcattttc ctcctcaact attggaagta gcatggcatt acatactcca tgaggtaaat
     2341 catataaacc tcctaattga tgagccatag catgtacatt tccaagacct gcattactaa
     2401 aggcaattcc atttaaaaaa caagcataac acatttgttc tctagcttct atgtcattac
     2461 catttttaac agctcttgga aggtatttaa atatctgctt tatagcatat aaggcagttg
     2521 aatctgtaac atcataagca ccattagcta ctacagcttc tacagcatga gttagtgcat
     2581 ccataccagt tgctgctgtt aaattagatg gtttgctaat cattaattct gggtcattta
     2641 ctgtcattgt agctagtgag ttggtatcaa ccattatcat ctttacatgt ctgtcttcat
     2701 cagttataac atagtttatt gtaacttcag cagatgtacc agcagtagta gttatagcta
     2761 cgataggaag acatttttta gaagttttgt tgataccttc atagtctcta atatctcctc
     2821 cattagtagc taatatagag atagctttac cacagtcttg tggagaacct cctcctatag
     2881 ttataacgaa gtcacaattt tctgatttta atattttaag tcctttatcc acattagtta
     2941 cggttggatt ttgtttaact tcatcataaa caacaaagtc tacatctatc tgagataaaa
     3001 tatctgttac tttttttaca gtaccatttg aaactagaaa tttatcacta acaacaaatg
     3061 cttttttagt tcctaatgat ttaattgaat ctttagcttc attaagacat cctttcccaa
     3121 gtaaatttat tggtggtaca taataagtgc tcataactaa aatctccttt tattttcatt
     3181 gatttttatg taaaaatata tatctaacaa ttttcactaa tttactcata atatatcgta
     3241 tcttagattg agatagggtt actggttctt atataagaac ttaattcgta attacgatta
     3301 tgattagaat ttaacatagt atttttatca tgtcaataaa aatataaaaa atattataaa
     3361 aaaaacaaag ttactgtagt taaatcagca actttgtttt tatagtataa atcaaataaa
     3421 tacattagtt taaaacaaaa tttttaatta ttatgttttg atatcttcca tatacagaat
     3481 attaatctaa tttattacaa tgtcttcatt acgttcttcc atcattgatt gaatattttt
     3541 ctgagcattt aagagtaatt tttttatcag ttctattttt tcgtcattag ctctaaataa
     3601 aggcagtgtt acactaacag cagctcttat acatccattt tttcttattg gtacggcaag
     3661 acattttagg tgttccattg attcttcaca ctctgaagct atttgtgttc tttgaacttc
     3721 aagaagctgg tcgtataaaa catcaaaatc tgtaattgat ttttctgtaa aaccttttag
     3781 accatctgga tataagctta ttagtttttc cttgtcaaaa tcacataaaa gagcttttcc
     3841 aagacctgta caattagcag gtattttttt tccaacataa gaaataagtc taattggttc
     3901 tggagaatct acttttccaa tataaagaac ttcattattg tcaagtatac caagctgaca
     3961 agtttcagaa caggaattaa ctatattagt catttcgtca attatatagt taaaaatagt
     4021 tcttttttca aaaaatgaag accctaatgt gtaagaacgt atccctatag aatatttgga
     4081 tgtgttttta tcaatagtaa tgtattttct agcattaagt gtatgaacta taggaaagat
     4141 gctacttttt ggagcattga taacatctgc tatttctgta agagtatatc cattttcgga
     4201 ttctgctaat gcttctaaaa tatctaatat cctagctgtt gggcgatgat tatctgaagt
     4261 cataaatttc cccctctttt tattaattta aagtattaga atatgttgat tttagcacac
     4321 tatacgatat tttacaatgt agaaaataaa tatgtaaatt tatatataaa taagctcttg
     4381 atttagatac agatagatat ttttttagac tgtacattaa ggaataacta atttccaaaa
     4441 tatacctaaa gaaagtaagt gatatttagt tatataattt aagtaaatat atgcaatagt
     4501 tatttttata tgtaaatatc aaaatattat gaatttatat tttatctatt ttttacactg
     4561 tataaaaact ttatagtttt gaatactata taattttaaa tacgctgtat ttttgcatat
     4621 aaggaagtca tttgataata tacaaggtat tatcagaaag tttgaaaaaa atttgacaaa
     4681 aatttatttt ttttattgac tcataaatag gaaaatgcta tcattaattc aaagattcgt
     4741 aattaaaaac ttagttctta aatacgaacc gaaacggccg ttagaattaa agctaaaaac
     4801 ttaataagag taattaaata tcatgtttta ggaggattaa ctttatgata atggatttta
     4861 ttaaaaaaat tcctgcaggt atgatgatag tgccaatgtt tatagcagct ttcattaata
     4921 ccttttgccc agatattgta caaataggtt cgtttacgac agcagtattt tcaagtgttg
     4981 ggtctgcaac tataattggg gttcaactgg tatgcttggg aactcagctt caatttagag
     5041 aaatgccaaa agttttaaag agaggtggta tacttcttgg ttctaaattt gctataggtg
     5101 ctttgatagg tatacttata ggaaagctat ttggaatgga tggtgtcctt ggtcttacaa
     5161 cattggctgt tataagtgca gttacaaata gtaatggtag tatatatctt tcattgatga
     5221 cttcatatgg agatgaaaca gattgtgcaa ctatgagttt acttacttta aatgatggac
     5281 cattttttac attaatagct cttgggactt caggtcttgc aaatgtgcca ttgttgtcat
     5341 tattagctgc aatagtgcca atacttgttg gtatgataat aggaaatttt gacaaaaaaa
     5401 tgaaagagtt tttagagcca gggtgtaatt tactcgtacc ttttgtaggg tttgcacttg
     5461 gtagtagcat aaatcttgca agtatactaa aaggaggttt atcaggagta ttacttggat
     5521 taatatctgt atttgtagga ggtatattta tagtattctg tgataaattt ataggaaaac
     5581 gtccaggata tgcaggatgg gctgtagcta caaccgctgg aaatgcaatt gctgtacctg
     5641 ctgctgttgc attagttgac ccatcatggc aaccatatgt tgcaacagct acaacacaag
     5701 tggcagccgc agtagttgtc actgcaatat tagtacctct tataactagt tggtgggcta
     5761 agaagtatgg atgtcctaag ttcccaaaac aaaatgagga tggtacaata acagcttaat
     5821 ttatattaca gtgagttgtt ggtaaaataa taaattcttt aattagattt taggatatta
     5881 ataaaattat attatttata aaggagagca agaataatga taaatgcagt gataattgat
     5941 gaaaaagata atgttgcagt agctattgaa gcaatagcta aaggtaatga aattagtttt
     6001 aaattaaaag acaaaacagt aaaaacgatt acagcactag ataatataac tatttatcat
     6061 aaattggcta taaaagatat ggcccaaggt gataaagtta ctaaatatgg tgaacatata
     6121 ggagaagcaa atcaggaaat aaaaagtggt caacatgtac atattcataa tgttagaagt
     6181 gttagagagg atttggataa acaagttatg actaatatat aaaataatat ttatagagtt
     6241 ataaaagttg tattcattgc ttttagtgtc aaatagtttt taaataggat tttagctgtt
     6301 tgattagtga gtgtaataaa aattataata tataaaaatt ataaaaagta tatagactat
     6361 tttaggagga aatcagtatg aaattttatg gatataaaag accagatgga agagttggta
     6421 taagaaacca catattaata ttgcctacga gtgtgtgtgc atcagataca actagaataa
     6481 tagcatcaca agttcaaggg gcagttacct ttaataatca gaatggatgt tcacaagtac
     6541 attcagacca acaattaact atggatgtta tggctggtta tgcagcaaat ccaaatgtat
     6601 atggtgtaat agttgtatct ttgggatgcg aaaattgcca aaatgatttg gtagttgatg
     6661 caataaaaga gagaacaaat aagccaataa aaactcttgt aatacaagaa gaacatggaa
     6721 ctttaaaaac tatagaaaaa gctgttcgtt atgctagaga aatggcacaa gaagcttctt
     6781 tgcttagaaa agaagaattt ccaatatctg aacttatact aggaactgaa tgtggtgggt
     6841 ctgacccaac aagtggatta gcagcaaatc cattaatagg tgaattaagt gataaacttg
     6901 tcgatttagg agcaacatca attttaagtg aaactactga atttataggt gcagagcata
     6961 tactagctag gagagcagta aatgaagaag taaaagaaaa aatattacat atagtacata
     7021 gatatgaaaa ttcattaaaa ttggttggag aagaagttag agaaggtaat ccatctcctg
     7081 gaaatatagc tggtggttta acaagtttag aagaaaaatc acttggttgt atacataaag
     7141 gaggtcatag acaaataagt gaagtatatg attatgccaa acaaattgat aagaaaggtc
     7201 ttgttataat ggatacccct ggaaatgatg cttcatctgt tgctggtatg gtagcaggag
     7261 gagcacaaat agtggtattt agtacaggaa gaggaacacc atcaggaaat ccaatagcac
     7321 cagtaataaa aataactgga aataaaataa cttttgcaaa tatggaagat aatatggatt
     7381 ttgatgctag tccagttatc tatggtccac aaacaatgga agaattaaca gatgatttgt
     7441 taaatatggt tgtagatgta gcaaatggaa aacaatcaaa agcagagtcc cttggatata
     7501 ctgaaatggc aatagctaga gtatgcaact atgtataatc tttaagtaag aaaataaata
     7561 catagaattt taataaaggg tgagattatg aagtttgtaa gttttactga aggagaaaat
     7621 gaatgtaaaa gaactggagt tttttctaaa gatgaattat ttattataga tgttaactct
     7681 ttaaatctca gtagaaattt taaggattta aatgaattaa ttcaaaatgt atctagtgaa
     7741 gatattatta agttgaaaac tattataaat gaaagtatat tctataatta tgatgttttt
     7801 aaaagagaga gatgtactgt acatgttcct atagaaaaac caatacatga tatcttatgt
     7861 gtgggagtga actataaaga ccatcttgaa gaaactcaaa ctcactttga tgatagtttt
     7921 gaagagccac aaaaaacggt ttattttaca aaacgtgtga caaaggcaat aggtccagaa
     7981 gatgagatag atggtcattt tgagataaat aatcaactag actatgaagt tgaacttgga
     8041 gtagtaatag gtaaaacagg tgttaatatt agaaaagaag aggtagaaga ttatatcttt
     8101 ggttacactg ttataaatga tatttctgct agaaagttgc aaaaagaaca tgttcaatgg
     8161 tttagaggaa aaggattaga tacatttacc tctttaggtc catgtatttt acataaaagt
     8221 gcagttcctt ttcccataaa acttaaagta tgtagtaggg taaatggtga agaaagacaa
     8281 agttctaata caggattatt catatcagat gtttcagaca tagtttcaga actttcaaaa
     8341 ggtatgactt tagaggctgg agacattata gctactggaa caccttctgg agtagggatg
     8401 ggattttcac ctcctcgata tatgagtagt ggagatgtag ttgaatgtga gattgagagt
     8461 attggaattt taaaaaatta cattaagtaa atgttttaat ataattaata ttaaagctaa
     8521 atctgatgta ttaatgaaca tattaaaata aattttagga gtttaaatta tgaaagcaga
     8581 atttttgaca ccagttgtta caatatttga tgaagaagga aatttagata aagaaggtaa
     8641 taaaaaagtg tacgaccatc ttatagatgg aggtgtagat ggactagtca taatgggaag
     8701 tactggagag ttctttaata tgtctatgga tatgcaaaaa gaacttatag atttagtaac
     8761 tagtcatgta aataagagag taaaagtgtt tataggtaca agtcgtatgt gtataaatga
     8821 atctatagaa ctttcaaact atgcacataa tagtggagca gatggcgtta tgataataag
     8881 cccttattat ttctctcttg atgatgatag tattgagtta ttttatagtg aaatagctaa
     8941 gaatacagag gctgaaatat atttatacaa ttatccagat agaactggtt atgatttaag
     9001 tccagaactt acattaagac ttgcaagaaa acataaaaac atagttggat ataaggatac
     9061 agtaactttg atgggacata ccagagattt aataaatact atgaaaatag agttcccaga
     9121 atttaaggtt tatagtggtt atgatgaaaa ctttatacat aatctaatgt ctgggggagc
     9181 aggttgtata gggggattat caaatttaat tccagaagtt tgctccaaat gggctgaagc
     9241 ttacaataat agagatatgg agaaggttat agaaattcaa aaatatatta ataaagcaat
     9301 ggatttttac ttaataacca atccatttat accagcaatg aaaaaagcta tgaatatcag
     9361 agggttacaa ataagtgatt atatgactgc tccatttgta aaaccaaatg aaaaacaagt
     9421 atgtgaaatt aaaaaattga tgaataaatt aaatatatgt taaatcaacc ttgcaaagag
     9481 gtcaacaata aattacaatt atcttaataa aaattaaatt aaaataatac tagggtacta
     9541 tattagttta atagggaaag tgatgaaaat tcactacagc ccccgctact gtatacggat
     9601 acgaaatcaa aatccactga aatttataaa aattttggga agggtgatgg aggaaaagcc
     9661 gtgagtcagg agacctgccc agtattatat aagttcaatt tagggtggag attgtaattt
     9721 ataataaaat gaagtatcaa ttcgtttttg taaagttgat atatgaactt ctccttatta
     9781 aattaggaga agttttttat tttattaaaa ggttatactt attaaagatg actttaggat
     9841 aactttaata agtatacttt aaaaagcata tattattaaa gttttttagc cactcataaa
     9901 aaataagata ttggagtgaa agagatgaaa tttaaatcaa atgttttaaa gcagtttttt
     9961 ttaattgcta tggtaatttt tatggtagga ggtcttatag ggtgtagtaa aagtgacaaa
    10021 gagaataaac aagctagtac tggtaagaaa ttagaattaa aatatgctag tggatttagt
    10081 gttgaagatt tagaagatgg aattaaaaaa gtaactgatg gagataatcg agaactgatt
    10141 ttaatcccaa agaagattaa aattcctgaa aaatataaaa atgctaatat agttagaacg
    10201 cctgtggaca atgtattaat agcatctaca acacaggctt gttctctgag aacaatagat
    10261 gaactagata gtataaaagc cgtaacaaca gaagagcagt actggactat taaagaaatt
    10321 aaagatttaa tgaaagatgg aaaaatacat tacgttggtt caaatttagc accagattat
    10381 gaaaaaatag tagacttaaa tcctgactta actatagtga gtagtggttt atcagaggct
    10441 gatacaaaat ttattgacaa attaaaagaa ttagggctta attatgttgt agacaatgag
    10501 tataaagagc aaaatccatt tggaagaatg gagtggacaa gtttaatagc tgcattttat
    10561 gataaagaag atatggctac tagtgaacta aataaaactg tacaaaaggt agaagaagta
    10621 agtaaaaaaa taaaaaacaa agctaaacct aaagttgtat gggcatctgt atatgaagga
    10681 agtgcttata tagctcctaa agattcatat gtggataata tgataaaaat ggctggtgga
    10741 ataaatgcat gtgcttcaat agattcaaat gaagggcgag taagtattga acaattgcat
    10801 gaagttgcaa aagatgcaga tatatttgta tactcatcaa gttcagatta tgcaccaaat
    10861 ttagattcta taaaatctag tgcacctgta ttggctgatt tggatgttgt aaaaaacgga
    10921 aacctatggg tgtttgctcc agattattat caatcagtag ataagacgga cgaattaata
    10981 gtagatttag ttgagatgtt ccacccaggt actacaggtg aaaaaatcaa acattatata
    11041 aattatgata aatagtacag agaggacaca aaaagatagg ttttatagat tttagttaat
    11101 ataactttgc taagaggagt atacaaatgg attttatcaa taaaatgaga ataaggaata
    11161 cgacaatatt tatagtatta attataagta tgatacttac ttttttagtg tgtgtagcat
    11221 ttggctctgt aaaaattgaa tttaaagaag taatagatgt tgtattaaga ggtatggata
    11281 atacagaaca ttataaaata atatacaata taagaatccc tagagtactt gcaaccatgg
    11341 taggtggagc atgcctagca gtatcaggta ttttattaca gatatttttt aaaaacccaa
    11401 tagttgaacc gtatatatta ggagtatctt caggttctac tttatcagta gctctcatga
    11461 ttttgggagg atacacattg ggatttgaga gtgtaaattc aatgagtata tttgtagcag
    11521 cttttatagg agctttaatt gtaatgtcta tagtcatttt atttgcaaat aaggtaaaga
    11581 atataacaac tcttttaata ataggaatta tgaccggata tgtatgtagt ggtataacaa
    11641 atgtgttaca atcatttgca aatagtgata aattaaaaga atttactatg tggtctatgg
    11701 gaagtttttc tggatttaca tgggaaaagg tagaaatact tataatagtt ggtatattag
    11761 gtcttttgat gactgtattc ataataaaac ctttaaatgc ttttttatta ggagaagagt
    11821 atgcaaaaag tattggcata aatataagat tttttagagt tttgatagtg tttatatcta
    11881 gtatattagc agcaatagta tcagcatttg caggacctat agggtttatt ggacttgcag
    11941 tgccacaaat ttcaaaattg attaaaaaat agctacgcac acttgtgact ttagtcatga
    12001 gttagtagct aaaatagtca gcatataggg aaacctatat gtagagatag gatagaacct
    12061 atccaatact acttgaattg ctggaaaccc ctaaagctaa ccaaactaaa acataaggat
    12121 gaaataaacc taagtgtgaa agttacgaaa gtagaaaaaa ttggttagat ggtataaggt
    12181 taaaccctaa gtactttaaa atgggcaatc agcaggtaag ctccgaatag gagaaacttc
    12241 aacgactatt cctcttgagg gaagtacact acaagctatt ggtagtggaa gtgggtagac
    12301 cttaacggat aatgccgaag gataagatat agtctgtgct tgcatgaaag tgtaagaagt
    12361 tcataagaga actgcatagg tagtagcgaa cttatgtgaa cgacaacctc aaaaatgata
    12421 aaagttaaga cttatggttc ttatatatgt tatttaaaat taaatataaa aaaccttcaa
    12481 aatacttgtt ttaagtaatt gcatatttta atattatgat aggtgatgaa aaatggaagt
    12541 tactcatggt agaggatatg catatagtat gtcaaatata gacagccagc attatgggga
    12601 gacatctttg gaatcctagt tatttcgttg ctactgtatc agaaaataca gaggagcaga
    12661 taagaaaata tattaataat cagaaaaaac aagtaaagtg aggtggtagt agtggaaaaa
    12721 gcttataagt ttagaatgta tccaaataaa aagcaacaag agttaattaa caaaactttt
    12781 ggatgttgta gatttgtata taataagtat cttgctaaaa gaatagatgt ttataaaaac
    12841 aataaagaaa cttttactta taaacagtgt agttctgatt tgaacaattt aaaaaaagaa
    12901 t
//
LOCUS       DAELSY010000112        12878 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.112, whole genome shotgun sequence.
ACCESSION   DAELSY010000112 DAELSY010000000
VERSION     DAELSY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12878
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.112"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(243..1280)
                     /locus_tag="KPG09_002778"
     CDS             complement(243..1280)
                     /locus_tag="KPG09_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG0967373.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(1299..2084)
                     /locus_tag="KPG09_002779"
     CDS             complement(1299..2084)
                     /locus_tag="KPG09_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG0967374.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(2117..3250)
                     /locus_tag="KPG09_002780"
     CDS             complement(2117..3250)
                     /locus_tag="KPG09_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG0967375.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(3298..4425)
                     /gene="hadC"
                     /locus_tag="KPG09_002781"
     CDS             complement(3298..4425)
                     /gene="hadC"
                     /locus_tag="KPG09_002781"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBG0967376.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKNETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(4425..5651)
                     /gene="hadB"
                     /locus_tag="KPG09_002782"
     CDS             complement(4425..5651)
                     /gene="hadB"
                     /locus_tag="KPG09_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBG0967377.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(5666..6466)
                     /gene="hadI"
                     /locus_tag="KPG09_002783"
     CDS             complement(5666..6466)
                     /gene="hadI"
                     /locus_tag="KPG09_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBG0967378.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(6505..7704)
                     /gene="hadA"
                     /locus_tag="KPG09_002784"
     CDS             complement(6505..7704)
                     /gene="hadA"
                     /locus_tag="KPG09_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBG0967379.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            8381..9379
                     /locus_tag="KPG09_002785"
     CDS             8381..9379
                     /locus_tag="KPG09_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBG0967380.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            9741..10268
                     /locus_tag="KPG09_002786"
     CDS             9741..10268
                     /locus_tag="KPG09_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBG0967381.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            10295..10891
                     /locus_tag="KPG09_002787"
     CDS             10295..10891
                     /locus_tag="KPG09_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBG0967382.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKDVIDTVSISLNAPNKEAYNRVTQPKFGEKSFDYMLGFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(10956..12539)
                     /gene="asnB"
                     /locus_tag="KPG09_002788"
     CDS             complement(10956..12539)
                     /gene="asnB"
                     /locus_tag="KPG09_002788"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG0967383.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
BASE COUNT         3988 a         2323 c         1644 g         4923 t
ORIGIN      
        1 tttaattaaa tttaagattt atttaaaaaa acaaagataa tccttacaga atataactca
       61 caatttgtaa gcaatataca tataaggatt atccagatta tcttgtatta taaaatttta
      121 ttcaaacaga ctttttaata actgacaaat acacactagc aataacttat caaagggtta
      181 ataattatgt tactaactaa taagacttat tcaatcagta atatttatat agtttgtatc
      241 tattaagctt ttatagcttc taataaatca tcaacgctat ctaatttttc tataagcatt
      301 ggaactattt catgtaagtc tccaactact ccatagtcag cgatttctaa aattggagct
      361 gctgggtttt tgtttatagc aattatgaaa tctgatgatt gcatacctgc taaatgttgt
      421 attgctcctg atataccaca agctatatat aggtttggtt taacagtagt tcctgtttga
      481 cctacttggt gagaatggtc tatccatcca gcatcaacag ccgcacgaga agaaccaact
      541 actccaccta atttgtcagc taatttctta agcatttcaa atccttctgg tccacctaat
      601 cctaaaccac ctgatactat aacatttgca tctgtaagag atactaaatc tttcttagtt
      661 ttaactgttt ctaaaacagt agttcttata tcatcttgag ttattttgta gtctaatgct
      721 ataacttcac cagttcttgt ttcgtctttt tcagctttat ccataacacc aggtctaact
      781 gtagacattt gaggtctatg gtttggacaa atgattgtag ccattatgtt tccaccaaat
      841 gctggacgag tttgttttat tttcttatct tctgggtcta tttctaactt agtacagtca
      901 gctgttaatc cagttccaac tcttgaagct attctaggtg ctaagtctct acctatatga
      961 gttgctccga aaagcattat ttctggtttt atttcatcta ttgcatcttt tattactttt
     1021 gtatatgcat cagttgtata tttttctaaa agagcatcat ctgcaacata aactttgtca
     1081 gctccaaaag tgattaattc tttagctaat ccatcaacat cttttcctaa taatattgca
     1141 caaagttctg catctacttc tttagctaat tttcttcctt ctcctaataa ttctataact
     1201 actggagcta tttttccttc tctttgttct gcaaatatcc atacgttttt ataagaactt
     1261 aaatctttta tatcattcat agtttgtact cctccaactt atataatgtg cttttgtttt
     1321 agaccgatta ttattttact agctatttca tcagctttac cttctaagat ttctcctttt
     1381 cctcttggtt caggagtaaa tgatctaaat actttagttg gtgatgcttt taaaccaact
     1441 tcttctttat ttacatctaa atcatcaatt gtccaaactt ttacatcatg tttatatgct
     1501 tttactattt tatcaacaga catgtatcta ggtgtattta actcttttac tgctgttaaa
     1561 agtacaggtg tacttacttt tattaattcg tatccatctt ctaattgtct ttgaactatt
     1621 atatcttttc cttctatttt gaaatcttgt acatatgtaa cttgaggaat gtctaatttc
     1681 tctgcaattt gtggtccaac ttgtgcagta tctccatcta ttgcttgtct tcctgcaaat
     1741 attatgtcat agtcacctac tttagatatt cccgctgcaa tagtatttga tgtagcccaa
     1801 gtatcagctc ctccaaatgc tctatcactt aataatattg catcatcagc acccatagct
     1861 aaacactctc ttagcataaa gtctgcttga ggaggtccca ttgttataac tgttatagtt
     1921 acatcatcat gctcatcttt aatttttaaa gcttcctcta atgcatttgc atcatctggg
     1981 tttaatatac ttggaactcc atctcttata agagtacctg tttctttatt tattcttact
     2041 tcgttagtat ctggaacttg ttttacgcaa actaatattt tcaaagtcta tacctccaat
     2101 ttcattagat tatttattat tttaatacgt ttgatgaaat taccattttt tgaacttctg
     2161 atgttccttc gtatattgat acgattctag catctctgta cattctttcg atttcataat
     2221 cttttataaa tccatatcca ccgtgaagtt gtaatgcttt ataagcaact tcatttgcaa
     2281 tttctgatgc atagtattta gccatagcag attcttgagc aactctcatt cctgcatctc
     2341 tcttttgtgc tgcatcataa actaatcctc tagctgcaca aacttttgtt tccatgtcag
     2401 ctatagtaaa ttgagtattt tggaatttag ctataggctt accaaattga actctttgtt
     2461 ttgtatattt aacagcttca tctaaagcac cttgagctat tcctagagct aaggcagcaa
     2521 cacctattct accaacttct agagtaccca ttgctatctt aaatccttga ccttcttttc
     2581 caagtaagtt ttcttttgga actttaacgt tttcaaatat taagtcagaa gtttcagttc
     2641 ctcttatacc catcttatct tcatgagctc ctgttgagaa accatcccat ttactttcta
     2701 ctataaacgc tgatgttcct ctaagtcctt tacttctatc tgtaactgct attactatag
     2761 ctacatcaca gaatggtcca ttagttatga aagtttttct accatttaat atatagtggt
     2821 ctccaactaa ttctgctgtt gtttgttgtc cacctgcatc agaaccagct cctggctcag
     2881 ttaacccaaa tgctcctatt aattcacctg ttgctatacc ttttaagtat ttttgttttt
     2941 gctcttcagt accattgtaa agtattggta atgaagcaag agaagaacca gctgtaacaa
     3001 aagtagctgt tgaagcacat cttctagcta tttcttccat tactatagcg taagaaacat
     3061 atcctgctcc cccaccacca tattcttctg ggatattact gcttataaat tttgcatcag
     3121 ctaatttttt gattaattcc ttaggcatta cacctgtttt atccatttct gctggtaaag
     3181 tatctaattc tttactaaca aaatctctta ctgcttttct taaaagttct tgttctttgt
     3241 tatataacat agcttcctcc aattttcata ttttatagta aatttatttt aatgaactta
     3301 cgctaaactc atcatctcag caaaagtttg aatacgagtt ctggcttgtt cattattctg
     3361 agtttgttgg tcgatttcaa catataaagt aggtattcca ctatcttcta tatcttttct
     3421 aactaaagga taatcgtatt cttctgggtc acagaatttc atcatacaga agataacacc
     3481 atctatatct ttctttttaa cttcatctac tataagtgac ccacgttttt tcttagggtc
     3541 ataagctaat gaacatcctt ctatgtttga ccattgtctt gctaatctct ctaacgcatc
     3601 atcacctgct ggtacatctg ttctgaattg tcttgtttct tgtgctaagt cgtcagctac
     3661 aactgatata ttgttgtctt ctaaaatgtc taatatatct ttagaatcag ctaatatacc
     3721 tgttaataaa actttttttc cagaacagac ttcttctggc atagcattta attttgctat
     3781 taaatctttc actagctcag tatgttcttc ttttctcata aagaacccac ttttaattac
     3841 taagcttctt attgatggtt ttatagtatt agaatgttta taagctactt caacaaagtc
     3901 tctcatagtt tttctatgtt cattgtaaac ttctatactt tcatgaattt ttgcttcttc
     3961 tatttcatat ccacaaattt cttcaagttc tctttttaca cctttgtact cactgattaa
     4021 gtattttaca ccagcttcaa gttttctatt ttgtgggtgt actaatgata tatattttat
     4081 atgaggtact gctgatttcc agttttgtcc taaacaaatt agtgtatcac acatacctgg
     4141 tataataact ccagataatt catcataagc accttttaat ccatattcta aacatgattg
     4201 cattattgaa catgcaaatg cagggaaata ttgtttagct aaatctaatt ctgtttgtcc
     4261 tccccatata ccaactggaa gcattccagc tgcatgtata atttcttctg ggcaataaac
     4321 tgggaaacaa cctatagctt ttttaccagt ttcattttta tattttttta cagccgcatt
     4381 tggattttca actacttctt tcattttaga taaaatagct tccattatat ctcacctcta
     4441 ttaagttttt ttctttcttc cattacttca actaaacctt gaattcttgt ttcaaattga
     4501 gcattagtga aagctctagg gtctgcttgg tcaccatcaa atccagcata tggtaatcca
     4561 gtttcttcag ctgctcttct ttgcatttca tattgtatta aactcataag tttacagctt
     4621 ctgttcatat gatagaatgc tccatcacat ttaccctcta ctaaagaatc aactctatat
     4681 tttgtcatac ggtctaggtt tacattgtta aacatagtac tatatgctac tgccattcca
     4741 tctaaatcat taacttcata ttgtaatgcc caagcatgtg ggtaaacact acctgtcatg
     4801 ttaactccaa atttagctaa tgttttcatc ttgtatccta tatatggcca acaaggtata
     4861 ccttccatca ttattctgta tttttcttcc cctctgaaag aagatttacc agttttcatg
     4921 ttgtcttcta attcttctat aagtaactta aatgcttctg tagtttcttt tttacctcta
     4981 gcacaaacta ttacagccat gtaagtaaat aagtcaaatc cattcattgg agaaggagaa
     5041 gaatctgctg gtaaactcat agaatacttc catagtcttc cgttttcagc tgatattttc
     5101 attacttctt caaacttctt agggtcaaat ttctttcctg atataatttc tagttgtttt
     5161 atagcttctt caaattgagc tttaatataa tctattcttg attgagtaac ttcgtcttca
     5221 ttattgaaag ttgtatcaat cattattaaa ggtatatcta attctcttga aatattttca
     5281 taccatttta taacttgatt acatatattg ttacagcaaa gtaggaaatc tggagctggc
     5341 atatccaaag cttcacatcc accattttct aaaagaccaa aatttgttct tgcatatgca
     5401 cataggtcaa tagaatatcc tttagattca gctatttcac ataattctaa agaacctttt
     5461 ttagctgcta ctccagctgc ttggttttct ggatataata cgtttaagtc aaatacttct
     5521 gctaactctt gaggaaatac tgatgttgac caacctacag gtcttccttc ttctttagct
     5581 ttaaatgcat ttgcatattg ttcagctaat aaatcattaa ttactactct agcttctttt
     5641 ttttcagaca ttttcatccc tctttttata tatttttcac ttctttttgt gattcttttg
     5701 cttcatcaaa agcatataac gctgctccta aagcaccagt taattgaggt atatcaggta
     5761 caataatttc tgtgttgatt tctcttgcca tagctcttac aataccacta tttctagcaa
     5821 ctccaccaac cataactaca tttctttgta ctcctattct ttttactagg ctagaaactc
     5881 tctttgctac tgaagtatga atacctgcca ctatatcttc aatttttgca ttttcagata
     5941 aatgtgatat aacctcagac tctgcaaata ctgtacatgt actgcttatt gatacttcat
     6001 tttgagaatt catagatata cttccgagtt cagatacatc aacctctatt atttttgcca
     6061 ttacatctaa aaatcttcct gtacctgcag cacatttgtc attcataaga aagtttaata
     6121 gtcttccatt attatctaat tttaatacct ttgcatcttg accacctatg tcaataatgg
     6181 ttcttgtctc tggaattatg aaatttaccc ctctagcatg acagcttaat tcacttattt
     6241 gcttatcagc atctgaatag ttcattcttc catatcctgt aactacaact tttttaatat
     6301 cttctcttgc aagacctgtc ttgccatata atttttctaa aactcttgat ggtccagtag
     6361 tcccagtacc agaggatatt gtttcagaag ctacaatatc ttccccattc tttaagatta
     6421 ctccctttga tgcagttgaa cctatatcta atcccattgt gtacatcttt agcattctcc
     6481 ttttaaaaaa tctagaatta ctaattaata tcttacaact ttactatctt taaagttatt
     6541 tattttctct tcatcgtagc ctaaagattt taatacttct acagtatgtt gacctacttt
     6601 tggtgctggt gtatattctt taattccttc atttctaaac ataactggag tattaactaa
     6661 gacacctgta tttccgctat cgtatgtttt cttaaataag aagtcatttg cccaagcttg
     6721 ttcgtcatct aataaatctt cacagctttg aattttttca aatggtaagt ctgcttcttc
     6781 taataaagct gaccactcgt ctaatgtttt ttctaacata gcttctccaa ctatcttaac
     6841 taaatcttca acatgattaa ccattgaatc tatgttatta tatctatcat cttctaatat
     6901 atattctcta tttataacct tacagaattt gcctaaccac ttgttgtatt gtattaaagc
     6961 taattgaatc catcttccat ctttacattt atatgtagtc attaatgggc tgtttggatt
     7021 ttctcttgat aaaggcattt catttccgta ttgtgctgtt gttatcattg ttcccattcc
     7081 atatatagct gtatggaaaa gacttactgt tactctctca cctttaccag tttgagcttt
     7141 tttatgtaat gctgctaaac ttcctgctgc tagtgctaga cctgcatagt ggtcaccaaa
     7201 tcctgctgct gtatttgctg gagatgttcc tttttccata acagattggc taactcctcc
     7261 tcttgcgaag tatgcagtat agtcaaatcc tggtttatct tttaaaggtc ctttttcacc
     7321 atatcctaat atttgagaga atattaatcc tggatactta tcttttattt ggtcataagc
     7381 tatacccatt ttttctaatg cttgaactct aacattagtt acaaatatgt ctgcttctga
     7441 taataattta tgtaatattt ctactccttc ttttgatttt acattaatac ttacaccctt
     7501 tttatttcca ttttctaatt caaacatagg gttttcatca tctgatgctg gagatttaaa
     7561 tgttccaccc ataactctta ttccatcacc ttctataggt tcaatcttaa taacctctgc
     7621 accccagtca cctaacattt ttgcacaaca tggtgctgcg atgaaacttg aaagttctac
     7681 tactttaact ccttctaaaa gcataacgtt ttcctcctta atagtatcaa ttataaaatt
     7741 tcgattaaat cactcataaa atactttttt ctgatattaa ctgtctgatg tcaaagttcc
     7801 taatcttatc taaaataact taaaaattat taaaattatt ttttattgtt ttattgtctt
     7861 acacctatat tgttaagcat ttatcgtgcc aactttatat ttaatcaaaa tatcgcttga
     7921 tttgaatgtt ttttaacaat atgcaattga ataatctttt tcatatcgtg gcatcttgcc
     7981 actatccatt gcattttgct attctttcta tgcttataaa agtttttttt atatattttt
     8041 ttatgaaaaa gcttgacatt tcaaaaaaag ttttcattgc taaactcaaa aacacatgtt
     8101 atcattgtaa ttaatcagca agttagttaa ctaatttcaa acttagataa ttttttaata
     8161 atatttaatc taaaaaataa caatttcttt gataaatttt agagtaatta ttacattaaa
     8221 taaaaaatta ctaaatatca aaataacaca gggaaaacaa tttcaaaact aaatttttaa
     8281 ccttaaaaat tttgtataaa aaatataacc ttatatgcta catattattt ctaaatgaaa
     8341 aattaccata ataatttaat aattacagga gggtattgat atgaaaatac tagtatttgg
     8401 agcacgcgat tatgaagaac cagtaataaa aaaatggtct gaagaacata aggatgttca
     8461 agtggatatt tatcctgaaa acatgactga agaaaatgta gttaaagcta aagggtatga
     8521 tggtatatct atacaacaaa ctaactatat agataatcct tatatttatg aaactttaaa
     8581 agatgctggg gttaaagtta tagcttcaag aactgcaggg gttgacatga tacattttga
     8641 tttagttaat gaaaatggac ttatcgttac aaacgttcct tcttattcac ctaatgcaat
     8701 agctgaatta gctgttactc aagctatgaa ccttttaaga aagactcctc tagtaaagaa
     8761 aaaagtctgt gaaggtgatt accgttggat agctgaactt cttggaacag aagttagatc
     8821 tattacagtt ggtgttatag gtacaggaaa aataggtgct acttctgcaa aattattcaa
     8881 aggcctagga gctaatgtaa ttgcatttga ccaatatcca aatagtgatt taaatgatat
     8941 attaacttac aaagattctt tagaagacct tctaaaagaa gctgacctta taacattaca
     9001 tactccttta cttgaaggaa caaaacatat gataaataaa gatactctag ctataatgaa
     9061 ggatggagct tacatagtaa atactggccg tggtggttta attaatacag gggatttaat
     9121 agaagcacta gagtcaggaa aaattagagc tgctgccctt gatacatttg aaactgaagg
     9181 attgttctta aacaaaaaaa tgaatcctgg agaattaact gacccagaaa taaataaact
     9241 tctttctatg gaacaagtta tattcactca tcaccttggt ttcttcacta gtacagcgat
     9301 tgaaaatata gtttattcta gtttaagtag tgctgtagaa gttataaaaa caggaactgc
     9361 tactaataga gtaaattagt attttataaa tatatttttt cattaattag ctattatcta
     9421 acaaaatagc ctttaatttc ccaaatataa gagagtcagc tcagtatata ttgagtagac
     9481 tctcttatat tattttttat attaaacttg tactcctctt atttatacca aattaaattt
     9541 atattaactt actctaacta ctcctactct atacatttta cctattttta cttttctaat
     9601 tatatacatt tgaatgggta caatctgtat tttacaaaaa aaacacctaa caaactatga
     9661 aaattgtatg attaatatta atatatttat aagaaaacat aacaattatg ttagctctgg
     9721 atttttaggg agggagtata ttggaaaaaa tgaataaagt tacttctaaa aaaactacct
     9781 atattgttac ttcagcatta tttgcatcca taatatgcct gacaatagca tatatattac
     9841 atattccagt tggtggaaac aatggatatg ttcatatagg agatgctttt atttatctag
     9901 ctgcaactat tttgccaaca aactatgcta tagctgcttc tgcaattggt gctggtctag
     9961 ctgatttatc tacaggtgct gctatctggg taataccaac tataatcata aaaccaatac
    10021 ttgtattatt tttcacttct aaaagtgaca agataataaa taagagaaat atagttgctt
    10081 cggttgtagc aggtatagta ggattagttt tatacatgtt tgctgaaggt ataattattg
    10141 gaagttttac aagtgcattt gtaatgagct tacttggctt attacagcca ataggaagtt
    10201 ttattgtttt tataatctta ggtatggcac ttgataaact tgattttaaa aaacgttatt
    10261 ttaattaaga atttaaattt atggagggaa aattatgaat atcttatata caattgaaaa
    10321 tagtatctat gtaaatataa ctaatacatg tccatgtagt tgtgtttttt gtataagaaa
    10381 cgaaaaagat gaagttgcaa atagtggtag tttatggttg gagcatgagc caagtgtaga
    10441 tgaagtaaaa gaagccttca ataaatacaa tttagacgac tatgatgaaa tcgtattttg
    10501 tggatatggc gaaccattaa tgagaataaa tgaattgata gaggtagcaa agtttataaa
    10561 agaaaaaagt agcataaaaa taagaataaa tacaaatggt ctaagtgatt taatacataa
    10621 caaaaaaaca gctattctgt taaaagatgt tatagataca gtatcaataa gtcttaatgc
    10681 cccaaataaa gaagcttata atagagttac tcaaccaaag tttggagaaa aatcatttga
    10741 ctatatgtta ggttttgcaa gagattgcaa aaaatatata aaagaagtag ctttttcagt
    10801 agtagatgaa atatctccag aagaaataga agaatctaaa caactggcaa aaaaacttga
    10861 tataccatta agagttagac ataagaatta gcaagtaaaa ttataaaaca ttaagaatca
    10921 cctgaatggt ctttcaggtg attcttcctc ttgtattaac attttacaaa atagatttca
    10981 taccacacta ggaatgtgta aatcatccaa atctttctcc aattatcaga gtttccacta
    11041 atgtagtcat taaaaatctc attaatctca tctatgttaa agaatttatc tgctatctca
    11101 ctattgaatt tatctcggat atccttatta tattggtcat cagccatcca tatacgaatt
    11161 ggcacaataa atcctaattt atttctaaaa gcaatctctt ctggtaatgt ttttgatgca
    11221 gctgtacgaa aggcaatttt attctctttt cctttcactt tatattctga tggtattctg
    11281 gatgcaatat caaatattct tgtatctgta agaggcattc tgacttccaa tccaactgca
    11341 gtactcattt tatctatatt aaaataaata tctccttcca accatatttg aatatctaca
    11401 tttgacatgt tagtaagtgt atctagttct tctgtttctt cataaattcc ttttaccact
    11461 tcaataggaa gcacatcagg attgtatttc ttaagaattt ttcttttctc accctcattc
    11521 ataatgtttg tatttccaat ataaaatgtc ttcaaattct ctccataaca ctctgcctta
    11581 tggtatatat tatatccacc aaaaaactca tctgctcctt ctccagagta acagatattt
    11641 gtgttttcag ctgctgcctt gcatccaata gcaaaaacga tagcagatgc atctccagtt
    11701 ggttgctcca tatggtacat tacataaggt actgacgcaa aatactcttc aggtgtaatt
    11761 atatatctcg aatttttaca cccaagaata ttagctgtct gttgtgctaa ttttgattca
    11821 tcaaaacgct cttcctcata accacatgaa cctgtttttt ctatatctga cattgccaat
    11881 atataagatg aatcaacacc tcctgataag aataactctg cagtttcatt ctctgatttt
    11941 acctctggca taattttctg aagtgttgta tgaatttcat ccgtccattc ctcaatagac
    12001 ttgctgtggt caggatgaaa ttcaggtttc caataacgac caatacatac tgttttattc
    12061 tgatagacaa ggtaatgtcc tggcataagc ttctttactc cacggaagaa tgtattttca
    12121 cctgctacat atgtgaaaga catataaatc tgtaacatgt cttcatttaa ctctttcaca
    12181 aatcctggtt gctccataat cttacgaata gatgaaccat agagaagctt cccattttca
    12241 gtctcataat aatagaaagg ttttactcca aactggtctc ttaggcagaa gagtttcttt
    12301 tccacagtat cccataatgc aaaggcaaac attccatgca tgtgatttgc catatcatat
    12361 ccccatgtct tatatgcttc cactaaaatt gctcgttcac gctcatctct acataatgaa
    12421 gaatcgattc caagttcttc acagagaact ttccagtttc gaatatgtcc attgtattct
    12481 agaattttta tcatatatgt gtcttttcgt gttaaatact tttcttctaa atttatcata
    12541 tgtgtatcct ttcgtattaa aacatttaac ttctaaattt attaaatgtt tatatatatt
    12601 tattcactta taatcactga aaaatcgact tttttcaaag taaattcttt ttgaattagt
    12661 actaatctgc tctttattaa tggcataaat atgattatca agtattatca gtgttataac
    12721 attgaatttt ttattcttca taaatattgc cccctttata ttaccaacta aatataatat
    12781 atcttatgtt acttacatat tctttaagaa attctgaaga ttttataaag ttttttgtat
    12841 tataattaat taaaaataaa attctagtta taaaacat
//
LOCUS       DAELSY010000113        12796 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.113, whole genome shotgun sequence.
ACCESSION   DAELSY010000113 DAELSY010000000
VERSION     DAELSY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12796
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.113"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      27..129
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            223..1599
                     /locus_tag="KPG09_002789"
     CDS             223..1599
                     /locus_tag="KPG09_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG0967384.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCLGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFM
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(1713..2858)
                     /locus_tag="KPG09_002790"
     CDS             complement(1713..2858)
                     /locus_tag="KPG09_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0967385.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     SRGNRSGNTISIITKKELDIIKDLQKEFSIVITPKTLQNGELIDIIK"
     gene            3110..3817
                     /locus_tag="KPG09_002791"
     CDS             3110..3817
                     /locus_tag="KPG09_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967386.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            3864..5459
                     /locus_tag="KPG09_002792"
     CDS             3864..5459
                     /locus_tag="KPG09_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0967387.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADSREKQKIMGMILSVVIITG
                     LLIFYVTSISLSLVDILKQINQMEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFFNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFMFIGQISSRIKYKNLILIIGSIILMLLYTLLMTRLNSMSVEMLKN
                     SVSISETIRKIYPPIYYFVDALKTGSVVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKTYKFKSQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIIGTILILL
                     FGADKVGELLKLNIDFNFIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLGFEIQFIIVSICLIASFSMFVALMGLLS
                     NLLYPNLDWKNEMAVVKRSASMIITMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TFILDFMIWRIISTKGVRIFKNL"
     gene            5562..6644
                     /locus_tag="KPG09_002793"
     CDS             5562..6644
                     /locus_tag="KPG09_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG0967388.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKEDKVNSNLDKVIEKLN"
     gene            6793..6954
                     /locus_tag="KPG09_002794"
     CDS             6793..6954
                     /locus_tag="KPG09_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBG0967389.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            7295..8563
                     /locus_tag="KPG09_002795"
     CDS             7295..8563
                     /locus_tag="KPG09_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG0967390.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            8758..9546
                     /locus_tag="KPG09_002796"
     CDS             8758..9546
                     /locus_tag="KPG09_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG0967391.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            9546..9995
                     /locus_tag="KPG09_002797"
     CDS             9546..9995
                     /locus_tag="KPG09_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG0967392.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            9999..12263
                     /locus_tag="KPG09_002798"
     CDS             9999..12263
                     /locus_tag="KPG09_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0967393.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKNALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            12403..12714
                     /locus_tag="KPG09_002799"
     CDS             12403..12714
                     /locus_tag="KPG09_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967394.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
BASE COUNT         4690 a         1390 c         2182 g         4534 t
ORIGIN      
        1 aattaaaata atattcgtaa agaaaaattc atgttttgta ctgtataaca tataattttg
       61 acaatatggg tcataagttt ctaccggaat accgtaaata ttctgactat gtatactatt
      121 atttagttaa ttaaatattt aattaactga attatgaagg atacggctca gcttatcaac
      181 tactgtgtcg tttttttgta aaatttaata ggaggtgttg ttatgcaaac aacaaacagt
      241 agtactatgc agaaactttt tccaattttg actaataaaa atgtcgacat gaagaaggaa
      301 ataattgcag gagtaactac ttttttaact atggcttata taatagctgt aaatccaaat
      361 attttatctg agacaggcat gcctgcaggt gcacttgtta caggtacttg tttagcagca
      421 gcatttggat gtattttaat gggtgtagtt gctaaccttc cttttgcttt agcatcaggt
      481 atgggtctta atgcattttt tgcatataca gtagttttac aaatgggtgt tccttgggaa
      541 gtagctctta cagcagtatt tgtagaaggt attattttta tagtattatc agtaagtgga
      601 gttcgtgagg ctgttgtaaa tgcgatacca aaaaatatga aattagctgt tacaggtggt
      661 ataggaatat ttattgcact tataggtcta gttaacagtg gaattgttat tggagaccaa
      721 gccactctta tcaaaatggg tagatttaca ccagcagtaa taataacttg tttaggatta
      781 ataattattg ctgtattaga taaaaaaaga gttaaaggtt ctatactttt tggaatagta
      841 gtgagttcat tacttgcttg ggcatttgct tttatgaatc cagagtatgc acaaaaatta
      901 ggtatatatt tacctggtgg attatttaaa ttcgaaagtt tagcaccaat agcaggtaaa
      961 atagatttag gatatgtact acatccaaca aatataggtg gttttttagt agtagtttgt
     1021 acatttttat ttgttgattt ttttgataca gtaggtacat tagttggtgt ttgttctaaa
     1081 gcaaacatgt tagatgaaaa agggaatgtt cctaatgtag gtagagcctt acttacagat
     1141 gcggttgcaa ctacagttgg tgcaggactt ggagtatcta cagttacaac ttatgttgag
     1201 agttcaacag gagttatcgc aggtggaaga actggatgga cagcaataac agttggaatt
     1261 ttattcttag ctgcaatgtt tttctctcct gtatttatag caattccatc atgtgctaca
     1321 gcaccagctt taatatatgt tggatattta atgcttggta cagttaagga tatagagttt
     1381 gataatataa ctgaaggtgt tccagctttt gttactattg cttgtatggc attaacatat
     1441 agtataggtg atggtttaac tttaggtata ttaacttatg ttttcgtaaa tatattttat
     1501 aatatatttg gagctaaaaa ggcagaagac aaaaaacatg tttctgtagt aatgattgtt
     1561 ctggcaatat tatttataat aaaactttgg ctattataat aggtattatt taaaatttta
     1621 atttaatata aaataaaggg ctataattta ttatcaattg tgatgataaa ctacagctct
     1681 tttgtatttg ttgtattttc gtatttattg tattatttaa taatgtctat tagttctcca
     1741 ttttgtaaag ttttaggtgt aattactata ctgaactctt tttgcaaatc ttttataata
     1801 tctaattctt tttttgttat tattgagatt gtgttaccac ttcgatttcc tctagaagtt
     1861 cgaccacatc tatgcaggta ttcattttta ctttttggaa aatccaagtt aaaaacatga
     1921 cttacatcca caatatctag tccacgagca gataaatcag ttgtaattaa aatctttgct
     1981 ttaccaagtt taaatttatt tattgcattc tttctatctt ctttttccat atttccaaaa
     2041 attcctattg ctttgtaatt atgataattt agttttgata ctaggacttc tatatttttt
     2101 tcattattta caaatacaat agcttttttt ggatttgtag ctgctaatgc ctttctaaga
     2161 aaagtaaatt tatcccttat ctcacctaat aaatatgagt gattgatgtt aggatttatt
     2221 tgtgattttt cctttgtttt tattatttca aactccttca taagtttatc acaaattttt
     2281 atagtgctat cagtaagact tgcagagcat cctataattt gtctatctct taacgttgtt
     2341 cttattatgt cttctataca agttatattt tttccattca atagattgtc cacttcatct
     2401 aagactatag tttttatatt atgtgatttt agcttctttt gttttatcaa gtctaaaact
     2461 cttccacaag agccaattac tatgtgtggt tttactgctt ttatattttt tatttgtttt
     2521 tgtatattga cctccccaat taaagctaaa gaagtaacgg agagttctgc atttttagcc
     2581 aataattcaa cctggtttgt aatctgcatt actaattcat gcgtaggtgc aagtataaga
     2641 gcttgtgttt ctctttttga tgtatcaatt ttttcaaata taggtaaaag gtacgctagt
     2701 gtttttcctg ttccagtctg tgaattaatc aataaatcct tattttgaat tatattccct
     2761 ataacaagtg actgaacttc tgttggagaa gttatgtctt gttttttaag tccatctatt
     2821 agagtggaac ttatttttaa ttgttcaaat gtattcatat tttgctcctt atatttaata
     2881 attatagtca actatttttt attatacatt gtttattcaa atcgaactat atattctttt
     2941 ataattatgt gtaatttgaa agtaaatatc aattatattt atacctttat atttctgata
     3001 cctttgcatt ttatagattt ctaaaataaa caaataaaag ttgtaattgt ttgatatttg
     3061 ttatatatta tatataacaa atatcaaaac tataatatgg agggaaatta tgttaaaagt
     3121 aatcaatgta tctaaatcat ataaaaataa aaaagtagta agtagtgtct catttgatgt
     3181 aaatgatgga gagctttttg ggtttatagg acataatgga gcaggtaaaa ctactactat
     3241 taaagcaatt gttggaatac acgattttga agagggagaa gtactaataa attctaaatc
     3301 tataaaaaaa cagcctgttg aatgtaaaaa ggaaatggca tatatacctg ataatccaga
     3361 tttatacgag tcattaacgg gtatacaata ccttaatttt atagctgaca tatttgaagt
     3421 tgccaaagac aaaagagaag agttaataaa atattattca aataagtttg aactcaataa
     3481 agcattagga gatttaatat cttcatattc gcatggtatg aaacaaaaat tagctataat
     3541 ttcggctttg atacacagcc caaaaatctt aattcttgat gaaccttttg taggtcttga
     3601 cccaaaagct tcctttatat tgaaggaaat tatgagggag ttttgtgaca aaggtggttg
     3661 tatatttttc tcaacacacg ttttagaagt tgctgaaaaa atttgtgata agattgccat
     3721 aataaaagat ggaaatataa ttgcttatgg aactacagaa gaagttaagg gaagtaattc
     3781 acttgaaagt atctttatgg aattaataga gaaataaaat gttttttata caatatagtg
     3841 tctaaagtga ggaatgataa aatatgagca atctaagtat attaataaaa actaatataa
     3901 taaatgaatt taaattaaat gcgttaaaaa aagctgatag tagagaaaaa caaaaaataa
     3961 tggggatgat actttcagtt gttattataa cagggttatt gattttttat gtaactagta
     4021 taagtctttc tttagttgat attttaaagc aaataaatca gatggaaatg ctacttatat
     4081 ttggatttgg attatcaaca ttaacaacta ttgttacatc attatataaa acgtcatcat
     4141 atctgtttca agcaaaagat ttagaccttt tgactagttt acctataaaa gaatctactg
     4201 tactagcaag taagatatta atgttggttg gagcaaacta tttattttct tcaatatgta
     4261 tgattatacc agcagtggtt tattttttta attctgatgt aagtataacg tatttccctt
     4321 atttgatact gctattttta gctttaccat tattaccaat agttgcttca tcaataattt
     4381 ttatgtttat aggacagata tcatcaagaa taaaatacaa aaatttaata ttaattatag
     4441 gtagtattat attgatgttg ctttatactc tacttatgac aagacttaac tctatgtcgg
     4501 ttgagatgtt aaagaatagt gtatctattt cagagacgat tagaaaaata tatccaccta
     4561 tatattattt tgttgacgct cttaaaacag gaagtgtagt ttccttttta gcatttatag
     4621 caatttcaat aataccattt tttatgtttg taacattatt tgcaaaaaaa tttaaagata
     4681 taaatgctaa gatgagtgaa ggatataagt caaaaactta taaatttaag agccaaaaaa
     4741 cttcaagacc ttcaaaagct ctattaaaaa aagaatttaa gagatatact tcttcattta
     4801 tatacttttt aaattcatct gcaggtttgt tattcctaat aataggtaca attttaatat
     4861 tattatttgg agcagataaa gtgggtgagt tactgaaatt gaatatagat tttaatttta
     4921 taaaaataca attattggga gtattcttct ttataatagt aatgaattgt acaacatact
     4981 gttcaatttc tcttgaagga aaaaatttat ggatactgaa atcttctcca atagatgaaa
     5041 gagaaatatt ctggtcaaaa atattgatga attttttatt gaatgcacca ttatctattt
     5101 tatgtttaat attagtatct ataagattag gttttgaaat tcagtttatt attgtatcaa
     5161 tctgtttgat tgcaagtttt tcaatgtttg tggcattgat gggattatta tcaaatttat
     5221 tatatccaaa ccttgattgg aaaaatgaga tggctgtagt aaaaagaagt gcaagtatga
     5281 taattacaat gttagtatca ttagcttacg taattatact tggaggtata tatgtattac
     5341 ttggaatttc gtttttaaat atatatatta ttttggcaag tatacttact tttatattag
     5401 attttatgat atggagaatt atttctacta aaggagtaag aatatttaaa aatttatagg
     5461 tgtatttatg tcaaataaag acttatcttt tttagtcttg caaataatag gttaagtagt
     5521 atactatgta tagtgatatg aaaaaattta ggagaaaata aatgagaaaa ttatccaaaa
     5581 gagaaaaaga ttttattcaa tatggatttt tattattttt gatatgtatt actacttatt
     5641 tagtattaac tactttagat ataaaactaa tacctaaaat tataaggcta gtaaataaga
     5701 cttatatatt tcttggcata ttgacaatta tagtatatat gataattgaa tctattgtaa
     5761 ctcatttgat aataaatgca gttcaaaaaa caaaagtaaa atttattggt attaaaatgg
     5821 ctacaatggg attttactat aacttagtta ctccatttgc atctgggagt caaccaatgc
     5881 aaatatatgc acttacaaaa tatgatgtaa gccttagtaa atcagtggct atagttacta
     5941 ataaaactgt agtatttcaa agtacagtta ctgtattttg tggaatttta attttgctaa
     6001 ataagtcatt actgatggag caagtacatt ctgtgaaagt cttgataact tttggaatga
     6061 ttatgaatgt aagtatgttg ttaggtggac tattaatagt atttagtcca aaacaagtaa
     6121 aaatgatagc agaagttttg attaattttt tatctaaatt taaaacattt aaatttttag
     6181 attcaaaacg tgatagaata aatcattata ttgatgatta taattattct ataaaaatat
     6241 ttattaaaaa tgtaagaatc ttagttttaa gtatattact tactgtgtta caattaatcg
     6301 catatttcag tatagtttat tgtgtgtata aagcctcaaa tttaagagga gtttcatatg
     6361 tacatataat gacattacag gtctttttat atatggctat atcaccaatt ccaacaccag
     6421 gaaatgttgg agctaatgag gttacatttt ttactatgtt ctccaatata attccaaaag
     6481 aattattggg gtactctgta ctcttatata gtgggtttgt atactatttt atattaatag
     6541 tttcaggttt atttacattt ggaacgcatt atacattaaa ttcttggaaa aaggaagata
     6601 aagtaaattc taatttagat aaggttattg aaaaattaaa ttaataaaat aattagggaa
     6661 attctatata taaatatggt gttgagtaag ctatattata ttgagatatt tccctttttt
     6721 gattaaaatt atagaaattt gaaataataa gtaataaaca gaaaaagtga tattgttttg
     6781 aaaggagaaa ctatgaaaca agataaacaa aatgaaaaac aaagtgagat ttctaagata
     6841 aaacaaagca atgataataa acttgaaata gctcaagaat tacatgtaag taaaaatata
     6901 gggaatatgc ttggatatag aatagataag aaaaaaatga atagaccaag ttaatgaaaa
     6961 tgttgaaaat ggtacatttt aaaaaatata tataatgaaa tgttttattg ttaaatttaa
     7021 acttatttta atggaggaaa aagtcgaaaa atatctaaaa agatatggat ataaaaaata
     7081 ttaatatata tatactataa acttgacaaa atcaaccttt taataactag caaaaaatag
     7141 caaaattata taagcgttat ttaattatca aaaatgaaag ctaaattgca tgtcctaaaa
     7201 aaaatgattg ataacattta tttgtgtgct ataattatat agtaaaacaa taagtatatt
     7261 gcataatgta tgcaaatcgg gaggagagac ggacatggaa gcatctatac acacaattgc
     7321 cagcaataat ttattgataa tttttgctat tgtaagtata actggaataa tttgtagtaa
     7381 gcttagtgaa ataataaagg ttccagatgt agtgttatat ttacttgtag gtattttaat
     7441 aggaccatca tttttaaaat ttatagatat tcgtggattt caaatagaaa atcaactaat
     7501 acttacattt ggttctgcat ttattttata tttaggtgga aaagaaataa gtttaaaagt
     7561 attgagaaat gtgaagatat ctgtattctt attagctact ttaggagttg tgatatctgc
     7621 ttttataatg caacaagtaa taggatttac ttttgggata agtgcaatga ctgctttact
     7681 agcaggaact ataatagcat caacagaccc agccactctt gtacctatat ttaatcaggt
     7741 aaagataaag gatagggtaa aacaaactgt aataagtgaa tcagctttta atgatgcaac
     7801 aggagctata ctaacatcag cagtgttggc tgtaattttg tcaaataaat tttcattagg
     7861 tgaaaatatt tatgaactgg ggacaatggt tattgtcgga gttttagttg gtttaattac
     7921 aggaatactg cttttgaaat tagttaatga taaaccttat ggaatattta aagattttgc
     7981 accaataata tctgttattt cagttgtagt tgcatatgaa ttatctacaa aattaggtgg
     8041 aagtggatat atgtcttgct ttattgtagg tataataact ggtaacaaga aaaactttaa
     8101 aatttggtta agtcaaaaat cttatgatgc agatttttat gttgctgaaa ctttaggtac
     8161 tatttgtcgt atggcaatat ttataatatt gggtagccaa gttaatttgg ctcagttaag
     8221 taaatatttt atgccatctg taattacagt attagtatta atgtttatag caagaccact
     8281 ttgcgtttta gtttgtacac ttgtagacag ggaagcaaaa tggagtaaaa gtgaaatatt
     8341 atttatgatg tgggtaaggg aaacaggcgt aataccagca gcactttgtg gtattatttc
     8401 agcaatgaaa gttcctggat acgaagtgat ttcatctgta gtatttatga ctatattaat
     8461 tacattagta atacaaggaa gtactacaaa attagtagct aaaaaacttg gattattaga
     8521 agaagaagtg ataaatataa gtgaaaaagt atcaactttt taaagttttg ctttaaaata
     8581 gtacctgtta atggctattt taatattgtc taatgatgat tattgaaata gtcatttttt
     8641 tatgtataag taatttacag aatttacata ttagacatat ttattatcaa aatttgttga
     8701 agctatgata taattagttg tatttaaaat aagaatagca aataagggag gaaatttatg
     8761 tttacggtta tggatattgt acagccagat acagtagaag aagcgtactc aattttaaat
     8821 aaaagaaaaa ctaaccaagt aattggtgga agtgcttttt taagaatggg gaaaaaaaga
     8881 ataggaacag gaatagaatt atctaaatta aatttagatt atataaaaga gtatgaagat
     8941 tacgttgaga ttggttctat gactactttt agaacattag aaacaagttc cataataaaa
     9001 aataattttg gaagaataat agaagattca gttaaaaata taataggagt tcagtttaga
     9061 aatgtagtaa cagttggtgc aacagtattt tctaaatatg gtttttctga cttgatagta
     9121 gcattacttt cattagatac ggaagtagaa ttatataata ttgggagaat tagtcttgaa
     9181 gagtttctaa atagagatta tgagaaagat ttacttatta agatatacat aaagaagaca
     9241 aataagaatg cttcttataa atcacttaga aatgcaaaga gtgattatcc aattctaaat
     9301 gtatctgtat caaaacatat ggaacaattt aagttatgtg taggagcaag accgcagaaa
     9361 gctacgattg caaaacaagc tagtgaattt ctatctaaca atgaaatcaa tgaaattaat
     9421 atagataaag cagtagaaat agcatcagaa gaactaactt ttggttctaa catgagagca
     9481 tctagggagt atagaaaagc tatgagtaaa gtacttttaa aaagagctat aatggaggtt
     9541 atttaatgtt agtaaagatt aatataaatg ggaagaaaaa aagtgttgat atagaaccag
     9601 aagaatatct ggttgatact ttaagaaaag ttggtaattt aagtgtgaaa agaggttgtg
     9661 acacaggctg ttgtggttta tgtacagttt taattgacaa gaaacctaca ttatcctgtg
     9721 caacactgac attgagagct ttagataaag aaataactac aattgaaggt ttagaaaatg
     9781 aagttaaaga atttagtgaa gttttggtaa aagaaggagc tgagcaatgt ggtttctgtt
     9841 ctcctggctt tatacttact gtacttgcaa tgaaggatga gcttgaaaat cctacagatg
     9901 atgaaataaa gcattatctt acaggaaatc tatgtagatg tacagggtat atgggacagt
     9961 taagagctat caaaacttat ctggaggtaa aatagaacat gaatatagtt ggaaaaggaa
    10021 tcaagaaaat agatggaatg agtatcacaa caggaaagcc tgtatataca gatgatttat
    10081 cagcaaaaaa tgctttggtg gtaaagatac ttagaagtcc tcacgcttat gcaaaaataa
    10141 aagatataga tacatcaaag gcagaaatgg ttgatggtgt tgaatgtgtt ttaacatata
    10201 aagatgtacc aaaatctcga tttacacttg ctggtcaaac atatccagag ccatctccat
    10261 atgatagatt aattcttgaa gatgttgtaa gatatgtagg tgacgaagta gctatagtgg
    10321 cagctataga tgaaaaaact gcaattaaag caatgaatct tatcaaagta aagtatgaag
    10381 tacttacacc tgttttggat tttgaagagg caataggaaa taaaactatg gtacatgatg
    10441 aagatagaca ttgtaacttt gatataggta tgcaaagaga aaaaaatata gtatcccatc
    10501 atgattatac aaaaggtgat gtagaaaaag tattaagtaa ttgtgatgta gtgattgaag
    10561 aaacttatta tactcaagct caagcacatg caatgatgga gacgtatagg gcatacaatt
    10621 atttagacca tatgggaaga ttggttgtag taagttcaac tcaaatacca tttcatgtaa
    10681 aaagacattt atctcgtgca ctagaaatac cagctagcaa aattagggtt ataaaaccta
    10741 gaattggtgg cggatttggt ggtaaacaaa ctgcgtgtgt tgagattttt tctgcaattg
    10801 ttactttaaa aacagggaaa ccagctaaaa taatttatga tagaaaagaa acttttaatt
    10861 gttcaacaag tagacatgca atgagattaa aagttagact aggtgctaca aatgatggca
    10921 taataaaggc aatagatata aaagcacttt ctgatacagg agcatatgga gagcatgctt
    10981 ctacaactat tggtttggtt ggagaaaaaa ctttaccaat gtataataaa gttgaagcaa
    11041 caagatttat tgcagatgtg gtatatacta ataagatgcc agcaggagca cttagagggt
    11101 atggagcatc acaaggatgt tttgctgttg aatcaactat aaatatgctt gctaataaat
    11161 taaaaatgga cccaacagat ttaaggttaa aaaatgtggt aacagaaggt gaaactacat
    11221 ttgcatatgg taaaacttta aatagtgtcg atttaataaa atgtataaat aaaggcaaag
    11281 aacttattgg atggaatgaa aaatatcctt gtaaacatct tgataatgga aaaattagaa
    11341 gtgtgggtat ggcaattact atgcaaggtt ctggtataca aggtcttgat aagtcttcag
    11401 ctgaaataaa attgaatgac tgtggagatt atactcttat gataggttct actgatatgg
    11461 gtacaggaag tgatacgatt ttagctcaaa tggctgctga ggttcttgat acaagcattg
    11521 aaaatataac agttatatca gctgatactg acttagttcc atatgaccca ggttcatatg
    11581 cttcaagtac tacttatgta acaggtatgg cagtagttaa ggcagctcaa gacttaagag
    11641 aaaaaataat ttctgctgga gcaactaaac taggtgttga gaaggatgaa gtagaatttg
    11701 atggtataaa attaactagt aaaaaaggta gcactttatt attcgatata gcagttgatt
    11761 ctacaattgg tccagatact aatcaattaa taggtcttgc ttctcatggt agcccagtat
    11821 ctcctccacc ttttatagca ggttttgcag aaactgaaat tgacacagaa actggaaaag
    11881 ttgatttagt aaattatgta gctgtagtag attgtggaac ggttataaat aaaaatctag
    11941 ctaagataca agttgaaggt ggaattgttc aagggattgg aatggcatta tttgaagaag
    12001 ttagatacac agaaaaagga agcatggaca caaatacatt tatgcaatac aagattccag
    12061 ctagatgtga tattggaaat gttaaagtag attttgttga aacttatgaa ccaactggac
    12121 catttggtgc taaatctgtt ggagaagttg ttataaatac acctttacca gcaatacaag
    12181 aagctgtgtt taatggaatt ggtgtaagaa taaatacact ccctattaca cctgaaaaag
    12241 tttttatgga aatgaataaa taattattta tgaaaattta gactagtttt acatgttaaa
    12301 caaccagtta tgtcatacct gttgaaagat atttaataag tagttattat aatattatga
    12361 attttactta taaaatacaa aattaaataa ggggatgatg ttttggctgg taatctaaat
    12421 aatatgagag cagtaaataa ttttagagga gataagaaca ttttagaatg tttagtcagc
    12481 tttgagggtc gttcaataag tcagagaaaa gtaagggtat tttttaaaga aaaacaaaat
    12541 caaatagaaa ttgattttgc agaagaggaa atttctaaat tggttgaaaa tgttgtttta
    12601 aatacatcat atcaagaaat gttatatgat gaaatagaga aacaactgga aattgattgt
    12661 ataggtactt ggatgatatt atctaaatta aaagatggta gtagagttca ctgattttaa
    12721 aataaattat tagagcctaa ttttaaggct cttttttgct gtttttaatt aacaatagaa
    12781 aatttatctt tagttt
//
LOCUS       DAELSY010000114        12661 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.114, whole genome shotgun sequence.
ACCESSION   DAELSY010000114 DAELSY010000000
VERSION     DAELSY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12661
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.114"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(189..374)
                     /locus_tag="KPG09_002800"
     CDS             complement(189..374)
                     /locus_tag="KPG09_002800"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBG0967395.1"
                     /translation="MEGFWRILKVEMYYLRKLDTYEQLVKTIEDYIYYYNNFRYQKRL
                     NSMSPLEFRKYLNTEIA"
     gene            complement(404..793)
                     /locus_tag="KPG09_002801"
     CDS             complement(404..793)
                     /locus_tag="KPG09_002801"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBG0967396.1"
                     /translation="MRAVGLSSVCRKKKFSYVKCTPEVIAENVLSRKFSADKTSQKWL
                     TGVTEFKLTNGMKAYLSAILDLDDRSIVSYVIGKSNNNNLVLETFDKAIELYPNAKPI
                     FHNDKSFQYTSKVFKSKLLTQGMIQSM"
     gene            complement(805..1265)
                     /locus_tag="KPG09_002802"
                     /pseudo
     CDS             complement(805..1265)
                     /locus_tag="KPG09_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888992.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1694..2704
                     /locus_tag="KPG09_002803"
     CDS             1694..2704
                     /locus_tag="KPG09_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967397.1"
                     /translation="MITKIKKTYIYLKEIQKIYKINEHKELVHIIKKLINDNKIKPIK
                     TSDLTPQHEQLYTKYRIIKQETEEDKKILEEINYKICDKLSIDYYRKNLVHYKNNRVA
                     IMDLNSYLIKKSDNLSKKISINERSYEIFKDEKFISSKVGKEVLKNLKIDLIKDLNVY
                     KTPEPFIYLSINRISPQKILIIENKDTYITITKMLLEGKEILKNKIDTVIYGEGKKII
                     NSIKGLKEDITIEYLTNKENEFLYWGDIDKTGLSIYTSLKDTNEINNIRLFEVAYEHM
                     IDKTKENTLRNIPKNQKDNFECGLENINNLNLKESIKNILVQGKYIPQEALNRFDLEE
                     YN"
     gene            2704..4323
                     /locus_tag="KPG09_002804"
     CDS             2704..4323
                     /locus_tag="KPG09_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967398.1"
                     /translation="MEMINILEGYNKRVNCIGSYYLIATLANNRNKFKEFDNIQFYNL
                     LIQVLCYIFDRSLRRKNCLRDDIKDFIEEINRMDYKIMLSEDDLKDLANYIINGLTNS
                     GKVYLFTYYSLEQEKHIDESIKIIEDKNVKINNQERLSYSLTTEGYRLLLSTKEYDEL
                     FQIQISQMIAKLRIEKGDYQGAKEDIYDIINTLEIHQQKIDNYIKIIKVNILYIKEEK
                     YSTVIDTTVETLLEEMDKYEELKKEVKLLIKDKEDYLEKDEDIDKEKIKNIRTQLLQM
                     KELIDGIVLAKSTATNLLSRVQDFREEFKEILKKLLTTPVIKRFNFKEEILDRLEKDS
                     DFLLIIRELYISLFKVELPNRFNIGVAYQEQKLLNTREIENEIVNKDELEIIENKEKE
                     ERINYCENYYFDFFKYLIKYGLEKGQFTLEEFLKNYLKYDKVIYLEITKDSKLMRDII
                     IYFANQRESINIKKIIRMLENSAFSPNTDFQIERLIERLLVEDFELLNSVSHIKSNKI
                     FEDEMKINTEINLETMTAVKLEIANIKFEII"
     gene            4341..5069
                     /locus_tag="KPG09_002805"
     CDS             4341..5069
                     /locus_tag="KPG09_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967399.1"
                     /translation="MNISLNDYKIANEIFYRLLKKSELKEKEAKELFLAYINNSNVRY
                     ALEAICEVSKIKIILISDTLYLIPNNDNDIFGFDYRRESLLGKDLKDSYLGYLIMTII
                     FSEFTNGVAPASYIQVLNLLDLVQESLERSIARDNLEEIEKSCEFNITILKNYWESKR
                     KWDESSKKGIETTSADFQIGFIRKIINFLKNEGLIVHIEDEDKIKPTRRFLDLMNNYF
                     LDEERKVLIEKLVLRKGESINASN"
     gene            5056..9411
                     /locus_tag="KPG09_002806"
     CDS             5056..9411
                     /locus_tag="KPG09_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967400.1"
                     /translation="MPVINKYKIINFKFGMGQERILSNRTMELYGENLQVEAENTAGK
                     TMSIQTIIQSICPLSDVAKPFTAIYNKKEPLYSMTEWLLDDGKTLLLTGIGFEKKAGS
                     SKEEGKEKKDDLYKYFMFIIEESKEMEVDIDNIPLLKNNDKGIRIVQPLTASQDYIKM
                     LRDKYGKNKVHFFNNYLRKQHREKLEEYGINQSEWKEIIIKLNSNNKEGGLSDFVKEH
                     NTSEKLIRDKILPLIETSLTTEEFAPILQIKEQVRKCINNIIDMNEKLSDYKNYRDLN
                     IRVESLQNKINRILFIEEDKNNVLGELANLYVHIENKRNELNEEIKQYQKMKASKEEE
                     FKIVEYEEDSYEYFRKEKEIQDTQVEFDKAKNELNDKIEELELDEQYLNISKFKRKKK
                     GLEEGEKDKIEKESEKQRKEVNQEEITAIINRLGSSIHYCLKEESIINNEKLKENTNN
                     NIAIQEKKNQLNDDKSKLTKVLISKNSEIKYIEGKERDFNNQLHEFKLNNTDIKGFIS
                     NNFLGEVIDYTRYINYLNIKVKSIEENLESKVMQIEESNKLKDDINEAINNLKLDNHE
                     SYMKLQVLKTRKKDIDNFKEKYKIIKTRFEIDNELDNSTKLYKEVKQELNKMKLQVQD
                     LENNKKQLEKELEDLQNYKDIIFEEEILTDLERNGIKPIEGMKYLLTLDGDLEYKNNL
                     ISKNPLLPYSIIITNKEYQKLKQINLKNKTKISMPIFVRDNIEDTFITTSSNLVISNG
                     LNILSSFDNKALDPEKREKDMNLIKEQINSIRDKVEQYKAEIEILEEFKLLTNKIDFT
                     AEDLSIDVKIENYKKIIFEINNKIKSNENNEKNLSNKIKELYENKEKLLNNKNILFNK
                     LKDIRNIQDTYSIILENKNEITKLKTSIEKIEEEKKVIKKSIEDIDVNIFNLNSEKDR
                     INRHIDDTESKINKFKIYEPIKLENAKLEELEIKYKKYLEDPTKKEIETLNYEISVCL
                     KRIESITIELEEFRQLISSDNFENIPILFTIEELNKRIVLNKSKVDELRKLKYSIKSD
                     IDKNQGSLNTKREEILKKFNGKEPLSTSLIVNFNFEERKIDIKKDIKDIGEIIEGNRT
                     TCQNLEQELRDLKKYKVERKAITYQPEDIFIESKKLQSKLQNVEYNLIKSINSYKNTI
                     LEIKEFNYKHMEKYKNFIETLNVNKDNYSRQKEQVEIVLLVIREEISKLEKHSEQLRK
                     EKTIISRQIKDYVMDCIDEFKIINKLGKHKGQALFNISLPKSDKIENSLSLVDMLMDE
                     IVEEANLGDVEQKINTFYILNKTLNIGRIPVSVIKYEINRNETIKWNSINNETTGGQR
                     FCISFIVTILLMEYKRYDRNAIVDESKYKGKVLLMDNPFGETSQQDFLKEIFELAKKF
                     NVQIISYTHVTNASIRAMFNKIYLMTVEKTTSNKEFVDINEIKQNKTDELVYMSKFYI
                     GNKEELIQEDIFSLI"
     gene            9513..9722
                     /locus_tag="KPG09_002807"
     CDS             9513..9722
                     /locus_tag="KPG09_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967401.1"
                     /translation="MKNFVIVSTKAALLRVIIVRLLLKIGLKTYKYLSYFMKKEKLKR
                     IIRPEGCELRVNRSIQVERAIAEQI"
     gene            10118..10258
                     /locus_tag="KPG09_002808"
     CDS             10118..10258
                     /locus_tag="KPG09_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0967402.1"
                     /translation="MKIKIKKYREKLLMIQNELAKSVGVRRETIVHLENGLMYHKGIQ
                     DK"
     gene            10396..11358
                     /locus_tag="KPG09_002809"
     CDS             10396..11358
                     /locus_tag="KPG09_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG0967403.1"
                     /translation="MAKIDNMMSILWMLNSDKKVTAKQISEKLEINIRTVYRHIDALS
                     ASGVPIISDTGHNGGYTLLNNFIKAPLFFDTEEQTALLHAAIFAKEEGSFLDDTLDKA
                     TSKLKMYSNQKQEETLTKHLIGFEVIKPTGKISIESVLKKLKNSIANELSVEINYRTA
                     HEEKSKCRIINPYGIVRWNNNWYIVAFCQMRNEIRSFRVDRISSVIETSNTFNRPKEF
                     SASEFFIRGIMDETKDEDNLISLVIEGKMNALDNLCQHWLLGYYLKERTSNKAIFKLQ
                     EEIIFTYVPNILIPYRKSIQIIEPLSLRDKMVEDLLELINHYQI"
     gene            11436..12065
                     /locus_tag="KPG09_002810"
     CDS             11436..12065
                     /locus_tag="KPG09_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBG0967404.1"
                     /translation="MKKRKVYMYVFDTMSDWEVGYLTAELNTGRYFKKGLKPLKVITV
                     GVDKNPIITMGGLKVLPEITIDELNIESNDLLILPGGNTWTSIGHEKILEKSKEALEQ
                     RSIVAAICGATLGLAKTGLLDFRNHTSNDLEFLKMVILSYSGEKYYKMESAVNDENLI
                     TASGIAPLEFTVQVLKVLDVFKEEALSSWLNLYKTHDSKYFFELMNCNK"
BASE COUNT         5577 a         1324 c         1847 g         3913 t
ORIGIN      
        1 gtgcttttaa ggttaataaa aatcttcttt atggcaatta tttaaatata atcaaatgtc
       61 gtaaaggaga ttttttatga acgctttaag aaaacaatta gccaaagaaa ttatatcaga
      121 tatctaaaaa agagcatgtc aaataaaacc ctaggtattt aaattttttt agagctactt
      181 aaaataaatt atgctatctc tgtatttaaa tatttcctaa attcaagtgg agacatagaa
      241 tttaatctct tttgatatct aaaattatta tagtaatata tataatcctc tattgttttg
      301 actaattgtt cgtatgtatc aagttttctt aaatagtaca tttccacttt taaaattctc
      361 caaaatcctt ccataggagc attatcaata caacgagcaa ctcttacata ctttgtatca
      421 tgccttgtgt taaaagtttt gatttaaata ccttactagt atactggaaa cttttatcat
      481 tatgaaaaat aggttttgca tttggatata gctctattgc tttatcaaaa gtttctaaca
      541 ccaagttatt gttatttgac tttccaataa catatgaaac tatacttcta tcgtctaaat
      601 ctagaattgc gcttaaatag gccttcatcc cattggttaa tttaaattca gttacacctg
      661 ttaaccattt ttgagaggtt ttatctgcac taaattttct acttaataca ttttccgcga
      721 tcacttctgg cgtacattta acataactaa atttcttttt tctgcaaaca gaacttaagc
      781 ctacggctct cattagtctg tacactctct acaatcttta acaatttcaa ctctttcatc
      841 taatgctgtt tttctagtct ccataaagat ttctcccttc gaggtttctt ttatctactt
      901 atgaggatta tactctttac cccaagcttt tagtgaataa actgatctca ttttatattt
      961 ttttgagatt gtgtcataag atccttcacc atttagataa gattttatag cattcaattt
     1021 aaattcttca caatagcata atttacaact ttgttttatt aaaccatcat ttccaaaagc
     1081 ttcataattt cgcatccact ctctgaatgt acgtttatcc aatatttaat tcttccatta
     1141 tttttattac tgtattctca ccttttaaat atgattcaat tgcttcgatt tttttgctct
     1201 gaagatatct tagttttaga tagtcttaca tattttaact gtctcctaag atgagtataa
     1261 ttcattcatc atcagttcta tagaatacac ctagttccta cttactgtag gaactaggac
     1321 tatatgaaac ccattcatag attgtagttt cttctgttgc attatagcgc agaatagtaa
     1381 attcatgtga aattttattt tgaatttact atttatatat aaatgaagag tggtcttatt
     1441 ttcaattaaa atttggaaca attttgggtt gacatctaca attaaggatt taagtttttg
     1501 ttatactaat aaataaaaac agagattcaa aatatataaa taatacttaa actagtgctg
     1561 atagattttc tttaagttct agttttttta ttgtcattat ctaatatgta ctattttatg
     1621 tacagtcaat ataatctgaa attgtaaaat ggtgtataat atagttataa gtaaagaagg
     1681 gatttaaaca tgtatgatta caaaaataaa aaagacttat atatacctta aggaaataca
     1741 aaaaatatac aaaatcaatg agcataaaga actagtacac ataataaaaa aactaataaa
     1801 tgataataaa ataaaaccaa taaaaacctc agatctaaca cctcagcatg agcaattata
     1861 tacaaagtat agaataataa aacaagaaac agaagaagat aaaaaaatat tagaagaaat
     1921 aaattacaaa atatgtgata aattaagtat agattattat agaaaaaact tagtacacta
     1981 taaaaataat agagttgcaa taatggattt aaatagttat cttattaaaa aatctgataa
     2041 tctatcaaag aaaatatcga ttaacgaaag atcttatgaa atatttaagg atgagaaatt
     2101 cataagtagt aaagtaggaa aagaagtact aaaaaactta aaaatagatc taataaagga
     2161 tttaaacgta tataagacac cagagccatt catatattta tcaataaatc gtatatctcc
     2221 acaaaaaata ttaattatag aaaataaaga cacttacata acaataacaa aaatgctatt
     2281 agaaggaaaa gaaatactta aaaataaaat agatactgtt atatacggag aaggaaaaaa
     2341 aataatcaac agtattaaag gattaaagga agatataaca attgaatatc ttacaaataa
     2401 agaaaatgaa tttttatatt ggggagatat agataaaact ggattatcaa tatacacttc
     2461 cctaaaagat accaatgaaa ttaataatat aagattattt gaagttgctt atgagcatat
     2521 gatagataaa acaaaggaaa ataccttgag gaatatcccc aaaaatcaaa aagataattt
     2581 tgaatgtgga ttagaaaata ttaataacct aaacttaaaa gaaagtatta aaaatatttt
     2641 agtacaagga aaatacatac ctcaagaagc tctaaataga ttcgacttgg aggaatataa
     2701 ctaatggaaa tgattaatat tttagaaggc tacaataaga gagtaaactg tataggatct
     2761 tattatctta ttgcaactct tgctaacaat agaaataaat ttaaagaatt tgacaacatt
     2821 caattttaca atttattaat tcaagttttg tgttatatat ttgatagatc tcttagaaga
     2881 aagaactgtt taagagatga tattaaggat tttattgaag aaataaatcg tatggattat
     2941 aaaataatgc tttcagagga tgacttaaag gatttagcaa actatataat taatggatta
     3001 accaatagtg gtaaagtata tctatttacc tactattctt tagaacaaga aaaacatata
     3061 gatgaaagta ttaaaattat agaagataaa aatgtaaaaa taaataatca ggaaagacta
     3121 tcctactcct taacaacaga gggttataga ttgttgttat caacaaaaga atatgatgaa
     3181 ctattccaaa ttcaaatatc acaaatgatt gcaaagttaa ggatagaaaa gggtgattat
     3241 caaggcgcaa aggaagatat ttacgatata ataaatacct tagagattca tcaacagaaa
     3301 attgataatt atataaaaat aataaaagta aatatactat atataaaaga agaaaaatat
     3361 tcaacagtaa tagatacaac tgtagaaaca cttttagaag aaatggacaa atatgaagaa
     3421 ttaaaaaaag aagtaaaact attaatcaaa gataaagagg actacttgga aaaggatgag
     3481 gatatagaca aagaaaagat aaaaaatata agaactcaac tattacaaat gaaagaatta
     3541 atagatggaa tagttttagc aaagtctaca gccacaaatt tgttatctag agttcaagat
     3601 tttagagaag aatttaaaga aatacttaaa aaactattaa caactccagt aattaaaaga
     3661 ttcaatttca aggaagaaat cttagataga cttgaaaaag acagtgattt tttattgata
     3721 ataagagaat tatatatatc tttatttaaa gtagagttgc caaatcgatt taatattgga
     3781 gtggcatatc aagaacagaa actattaaat actagagaaa ttgaaaatga aatagttaat
     3841 aaagacgagt tagaaattat tgaaaataaa gagaaggagg aacgtataaa ttattgtgaa
     3901 aattactatt ttgatttttt taaatactta attaaatatg gacttgaaaa aggacaattt
     3961 acattagaag aatttctaaa aaattatctg aaatatgaca aagttattta cttagaaata
     4021 actaaagact ccaaattgat gagagatata ataatttact tcgctaatca aagggaaagt
     4081 atcaatatca aaaagataat cagaatgtta gaaaatagtg ccttctcacc aaatacagat
     4141 tttcaaattg aaagattaat agaacgttta ttagtagaag attttgaact attaaatagc
     4201 gttagccata ttaaaagtaa taaaatattt gaagatgaaa tgaaaataaa tactgaaata
     4261 aatttagaaa ctatgactgc agttaagcta gaaatagcaa atataaaatt tgaaataatc
     4321 taaatataag gggaaataca atgaatatat ctttaaatga ctataaaata gccaatgaaa
     4381 tattttatag attacttaaa aaaagtgagt taaaagaaaa agaagctaaa gaattatttt
     4441 tagcttatat aaataacagt aatgtaaggt atgctttaga ggcaatttgt gaagtatcta
     4501 aaataaaaat aatattaatt tcagatacct tatacttaat accaaataat gacaatgata
     4561 tttttgggtt tgactataga agagaatctt tattagggaa agatttaaaa gatagttatt
     4621 taggatattt gataatgaca ataatatttt ctgaatttac aaatggggta gcaccagcat
     4681 catatattca agtattaaac ttactggatt tagtacaaga atccttagaa cgttcaatag
     4741 ctcgtgataa tctagaagaa atagaaaaat catgtgagtt taatataact atcttaaaga
     4801 actattggga aagtaaaaga aagtgggacg aatctagtaa gaaaggaata gaaacaacat
     4861 cagcagattt ccaaataggg tttattagaa aaataattaa tttcttaaaa aatgaaggct
     4921 taattgtaca cattgaggat gaagataaga taaaaccaac tagaagattt cttgatctta
     4981 tgaataatta ttttttagat gaagaaagga aagtattaat agaaaaacta gttttaagaa
     5041 aaggggaaag tattaatgcc agtaattaat aaatacaaaa ttataaattt taaatttggt
     5101 atgggacaag aaagaatact tagcaacaga accatggaac tgtatggaga aaacttacaa
     5161 gtagaagcag aaaatacagc aggcaaaact atgagtattc aaactatcat acaaagtatt
     5221 tgtccactat cggatgtagc caaaccgttt actgcaatat ataataaaaa agaaccatta
     5281 tactcaatga ctgaatggtt gctagatgat ggtaaaacgt tattattaac gggaatagga
     5341 tttgagaaaa aggctggatc atctaaagaa gaagggaaag aaaaaaagga cgatttatac
     5401 aaatacttta tgtttattat agaagaatct aaagaaatgg aggttgatat agataatata
     5461 cctttattaa aaaacaatga caaaggtata aggatagtac aacctttaac ggcctcacaa
     5521 gactatataa aaatgctaag agataaatat ggaaaaaaca aagtacactt ctttaataat
     5581 tacttaagaa aacaacatag agaaaaatta gaagaatatg gaattaacca aagtgaatgg
     5641 aaagaaatta ttataaaact taatagtaat aacaaagaag gtggactatc agactttgtt
     5701 aaagaacata atacttcaga aaaacttata agagataaaa tattaccttt aatagagaca
     5761 agcttaacaa cagaagaatt cgctccaata ttgcaaatta aggaacaagt aagaaagtgt
     5821 ataaataata ttatagacat gaatgaaaaa ttatcagatt ataaaaacta tagagattta
     5881 aacattagag ttgaaagttt acaaaataaa ataaatagga tcctattcat agaagaagat
     5941 aaaaataatg tattaggaga attagctaat ctatatgtac atatagaaaa taaacgcaat
     6001 gaattaaatg aagaaataaa acagtatcaa aaaatgaaag catcaaaaga agaggaattt
     6061 aagatagtag aatatgaaga agatagctat gagtatttta gaaaagaaaa agagatacaa
     6121 gatactcagg tagaatttga taaagccaaa aatgaattaa atgataaaat agaagaacta
     6181 gaattagatg aacaatattt aaatatatca aagttcaaaa gaaaaaagaa ggggctagaa
     6241 gaaggggaaa aagataagat agaaaaagaa agtgaaaaac aaagaaaaga ggtaaatcaa
     6301 gaagaaataa cggctattat aaatcgtcta ggtagcagta ttcattattg tttaaaggaa
     6361 gaaagtatta taaataatga aaaacttaaa gaaaatacaa acaacaatat agctatacaa
     6421 gaaaaaaaga atcaacttaa tgatgacaaa agtaagctca caaaagtatt gataagtaaa
     6481 aattcagaaa ttaaatatat agaaggcaaa gaacgtgatt ttaataatca actacatgaa
     6541 tttaaactta ataacactga tattaaaggc tttatttcta ataatttttt aggcgaagta
     6601 atagattata ctaggtatat aaattattta aatataaaag taaaatcaat agaagaaaat
     6661 cttgaatcga aagtcatgca aatagaagaa tcgaataaac ttaaggatga tataaatgaa
     6721 gctataaata atcttaaatt agataatcat gaatcttata tgaaactgca agtattaaaa
     6781 actagaaaga aagatataga taattttaaa gaaaaatata aaatcattaa aacaagattt
     6841 gaaatagaca atgaattaga taactctact aaactatata aagaggtaaa acaagagctt
     6901 aacaaaatga aacttcaagt acaggactta gaaaataata aaaagcaatt agaaaaagag
     6961 ctagaagatt tacaaaatta taaagatata attttcgaag aagaaatatt aacagatcta
     7021 gaaagaaatg gaataaaacc tattgaaggc atgaaatatc tattaacctt agatggagat
     7081 ttagaataca aaaataatct catatctaaa aatccattgc taccatattc tataataatt
     7141 acaaataaag agtatcaaaa actgaagcaa ataaacttaa aaaataaaac taaaatctca
     7201 atgcctatat ttgtaagaga taacatagaa gatactttta taactacatc tagtaacctt
     7261 gtaattagta atggattaaa tatactatct tctttcgata acaaagcttt agatcctgaa
     7321 aaaagagaaa aggatatgaa tctaatcaaa gaacaaataa atagtattcg agataaagtg
     7381 gagcaatata aagcagaaat tgaaatatta gaagaattta aacttctaac taataaaatt
     7441 gattttacag ctgaagactt atccatagat gtgaaaattg aaaattataa aaaaattata
     7501 tttgaaataa acaataaaat taagagtaat gaaaataatg agaagaattt atctaataaa
     7561 attaaagaac tttacgaaaa taaagaaaag ctattaaata acaagaatat attatttaat
     7621 aaattgaagg acattagaaa tatacaagat acatattcta taattttaga aaataaaaat
     7681 gaaataacta agttaaaaac aagtattgaa aaaatcgaag aagaaaaaaa agttattaaa
     7741 aaatcaatag aagatattga tgttaatata tttaatttaa attcagaaaa agataggata
     7801 aataggcata tagatgatac agaaagcaag ataaataaat ttaagattta tgaacctatt
     7861 aaacttgaaa atgctaaact agaagaatta gaaataaaat acaagaaata cttagaagac
     7921 cctacaaaaa aagagattga aacattaaat tatgaaatct cagtttgctt aaaaagaata
     7981 gagagtatta ctatagagtt agaagaattt agacaactta ttagtagtga taattttgaa
     8041 aatataccta tattattcac aatagaagaa cttaataaaa gaatagtttt aaataaaagt
     8101 aaggttgatg aactacgaaa acttaaatat agtattaaaa gcgacataga taaaaatcaa
     8161 ggttcattaa acactaagag ggaggagata cttaaaaaat ttaatggaaa agaaccttta
     8221 tctactagct taatagtgaa ctttaatttt gaagaaagaa aaatagatat aaaaaaagat
     8281 ataaaggata ttggtgaaat tatagaaggt aatagaacta cttgtcaaaa tctagaacag
     8341 gaattaagag acttaaagaa atataaagta gaaagaaaag ctataaccta tcaacctgaa
     8401 gatattttta ttgaaagtaa aaaactacaa tcaaaactgc aaaatgttga atataattta
     8461 attaaatcta taaatagcta taaaaataca atattagaaa taaaagaatt taattataag
     8521 cacatggaaa aatacaaaaa ctttatagaa actttaaatg taaataaaga taattactct
     8581 cgacaaaaag aacaagtaga aatagtatta ctagttataa gagaagaaat aagcaaatta
     8641 gaaaaacata gcgaacaact aagaaaagaa aagactataa tatcaaggca aataaaagat
     8701 tatgttatgg actgtattga tgaatttaaa atcataaata aacttggtaa acataaagga
     8761 caagccttat tcaacatatc actacctaaa tctgataaaa tagaaaatag tttgagctta
     8821 gtagatatgc taatggatga aattgtagaa gaagcaaacc taggtgatgt tgaacaaaaa
     8881 ataaatacct tttatatttt aaataagact cttaatatag ggagaattcc tgtatcagtt
     8941 ataaaatatg aaataaatag aaatgaaact ataaagtgga actctattaa taacgaaaca
     9001 acaggaggac agagattttg tatctcattt attgtgacaa tattgttgat ggaatataaa
     9061 agatatgata gaaatgcaat agttgatgaa agtaaatata agggtaaggt actacttatg
     9121 gataatcctt ttggagaaac gtcgcagcaa gatttcctta aagaaatatt tgaacttgct
     9181 aaaaaattca acgttcaaat tatatcctat actcatgtaa caaatgcttc tataagagca
     9241 atgtttaata agatttattt aatgacagtt gaaaaaacta cttctaataa agaatttgtg
     9301 gatattaatg agattaagca aaataaaact gatgaattgg tgtatatgag taaattttat
     9361 ataggtaata aagaagaatt aattcaagag gatatattta gtttaatata aataacaagt
     9421 aaacaaataa agtgattact atcagtgagg caggtagaaa ctgaattact ttgtgactca
     9481 ttacgactct tggggtaaaa ttgtgtataa atgtgaagaa tttcgtgatt gtcagtacaa
     9541 aagcagcttt attaagggta ataatagtaa gactcctctt gaaaatagga ctaaaaactt
     9601 acaagtatct aagttatttc atgaaaaaag aaaaattaaa gagaattata agaccagagg
     9661 gatgtgaact tagagtgaat agaagtatac aagtagaaag agctatcgca gaacaaattt
     9721 aattttatca tacgatagcc tcttgttatt ttagtgatga aatccatcaa gcataccata
     9781 actacagaaa aagaggcttt tctgatggta tattgggttc ttttagagtg tgtgatttgc
     9841 tacatgttca ttaggataat aatagtcagt aaatgatatt actgatagaa tagatataat
     9901 aaactaatct agttccatat tgtaatgttt attaaagagt atacttttat gattataata
     9961 gctattttct ttgaagctta ttgtacaaga gatttgaata tatcaaattt ggatataggt
    10021 ataattctct tagcagtttt atttatacag tctttatcta aattagtgtt gtatatactt
    10081 tgataaaagg gggatatgta actaagttgt aatattgatg aaaataaaaa ttaaaaaata
    10141 tagagaaaaa ctccttatga tacaaaatga acttgctaaa tcagttgggg taagacgaga
    10201 gactattgta cacttagaaa atggattaat gtaccataaa ggtatccaag ataaatagtt
    10261 aaaaaactag ataagaataa ggagccttta cagaacaaat ttagtgtgct cgtgcgatag
    10321 cccttttttt ataaaaatta atctaataac ctatataatt aaatttataa aacatatatg
    10381 aaagggtaaa actatatggc taaaattgat aatatgatgt ccatattatg gatgttaaac
    10441 tcagacaaaa aagttaccgc aaaacaaata tctgaaaaac tagaaataaa tataagaact
    10501 gtctacagac acattgatgc attatctgct agtggagtac ctataatttc agatacaggt
    10561 cataatggtg gatatacact tttgaataat ttcatcaaag cacctctttt tttcgataca
    10621 gaagaacaaa ctgcgttact tcatgcagct atatttgcta aagaagaggg aagtttctta
    10681 gatgacacac tagacaaagc aacctcaaag ttaaaaatgt attcaaatca aaaacaagaa
    10741 gaaactttaa caaaacattt aataggtttt gaagtaataa aaccaactgg aaaaatatcc
    10801 atagaatctg tacttaaaaa gttaaaaaat agtatagcaa atgaattatc tgtagaaatc
    10861 aactatcgta cagctcatga agaaaaatca aaatgtagga ttataaatcc atatggaatt
    10921 gtacgttgga ataataattg gtacatagta gcattttgtc aaatgagaaa tgagattcgt
    10981 agttttaggg tagatagaat tagtagtgtc atagaaacat caaatacttt taataggcca
    11041 aaagaatttt cagcaagtga attttttata agagggatta tggatgaaac aaaagatgag
    11101 gataatctta tatcattagt tatagaagga aagatgaatg cattggataa cttatgtcag
    11161 cattggcttc taggatatta tttaaaagaa aggacttcaa ataaagcaat atttaaactt
    11221 caagaagaga taatatttac atatgtaccg aatatactta taccttatag aaaatctata
    11281 caaataattg aaccattaag ccttagggat aaaatggttg aagatttact cgaattaatt
    11341 aatcattatc aaatttaata acttaactga cagagtatgt cagttaagtt attttacaat
    11401 taggtatatt aattaattat agaaggagca aaaaaatgaa aaagagaaaa gtatatatgt
    11461 atgtattcga tactatgtca gattgggaag ttggatatct aactgctgaa ttaaatacag
    11521 gaagatattt taaaaaagga ttaaaaccac taaaagtaat tacagtagga gttgataaaa
    11581 atcctattat aacaatgggt ggattaaaag ttttacctga gattacaatt gacgagctta
    11641 atattgaaag taatgactta ttaattttgc cagggggaaa tacttggaca tctataggtc
    11701 atgagaaaat attagaaaaa tctaaagagg ctttggaaca aaggtctata gttgcagcta
    11761 tttgtggtgc tactttaggg ttagctaaaa caggtttact agattttaga aatcatacaa
    11821 gtaatgattt agaattttta aaaatggtga tactaagtta ttctggagaa aaatattata
    11881 aaatggaatc tgcggttaat gatgaaaatt taattactgc ttctggaatt gcaccattag
    11941 aatttacagt acaagtttta aaagttttag atgtttttaa agaagaagca ttaagttctt
    12001 ggttgaacct ttataagacg catgactcta aatatttttt cgaactaatg aattgtaata
    12061 aataaattta agatttaatt ttaaattcaa attttaaatt actttttcta tggcaataga
    12121 taagtagttt agaattttat catatatata atttgtattt ataccaacac aaatagaaat
    12181 gatagttcca ataataaaat ttataattag ttccatatgg agtacatttc tttcctacta
    12241 atataactaa gaatatatga aaccaccata gattgtagtt ttttctgttg tattatatca
    12301 tgggatagta aagtgacgta tatttttgat gaaaacctag gattgtatat gaaatgaatt
    12361 aatcctgtgt attaatcttg agattcgtgt ctaatgattt gtaaaaataa ataatgaaat
    12421 acttagaaat acttgtaaat gctagtcaat attggctagt attttttatt ttagtaatga
    12481 aatccccaac tgtacttcat ccacaaaaaa agagatattt ttgagggtgt attggagtga
    12541 acttaggctg tataattata catttcaata tcttatatta attaggataa tagttggtaa
    12601 atgatatgat tgaaacaata gatataataa actaatctag tactgtactg cttactaaag
    12661 a
//
LOCUS       DAELSY010000115        12639 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.115, whole genome shotgun sequence.
ACCESSION   DAELSY010000115 DAELSY010000000
VERSION     DAELSY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.115"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(7..606)
                     /locus_tag="KPG09_002811"
     CDS             complement(7..606)
                     /locus_tag="KPG09_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBG0967405.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(624..3743)
                     /locus_tag="KPG09_002812"
     CDS             complement(624..3743)
                     /locus_tag="KPG09_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG0967406.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            3964..4788
                     /gene="pdaA"
                     /locus_tag="KPG09_002813"
     CDS             3964..4788
                     /gene="pdaA"
                     /locus_tag="KPG09_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG0967407.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            4977..5741
                     /locus_tag="KPG09_002814"
     CDS             4977..5741
                     /locus_tag="KPG09_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0967408.1"
                     /translation="MTTICVMIDHIFLDTEYRQPTIHKHLAKHIIISQSGNLSVTFDK
                     GESLQCKGIIIDSNVSHTVSSKDNKMLVFLIDDTSILAQNISQHQLLNHSFFVIDNLI
                     VERIQNIYTACQVKGLSQSYIDFSKQAFRLLNLNYHISSITDSRIQWALNHILKSTHI
                     HNEIFFEICAEVKLSKSRFSHLFKEQMGVSLNSYLALSKLRKAYELLLKSGDITSAAM
                     DAGFSSPSHFSASSKKYLGITASELIGKCKIYFIRQ"
     gene            5844..7022
                     /locus_tag="KPG09_002815"
     CDS             5844..7022
                     /locus_tag="KPG09_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBG0967409.1"
                     /translation="MKTNKISFNKGLYYLNTNANFNFQLNRIIMWDGGRLEDIQKVSS
                     KIVDSESWKTELMYLAQEAEQDGRTENAIAYYRMSEFFMYDGDPDKISVYKKATTLFY
                     NYYSEYFSNGTVQIFHVPYENITLPVMYAKAQGEVKDVILLHGGNDSYFEEFFFPMLY
                     LASQGFDIYLFEGPGQGGVMRLQGKHFTYQWERPVKTILDYFHLENITIVGASLGGFL
                     APRAAAFEKRIERVVAWSVFPNFLDIIVGSQPIKMQNLLRLLLKLKTKWLVNFIIKQK
                     MKSGDSMVVWGLKHGMYAYEASTPYEYFQKIKYYQIDSIAKNINQDILILGATQDHFI
                     DYRTVGKEIDLLNNVRSLTFRLMTEAEQASNHCNCGNSKLVLDTISNWIMMMKIHDRV
                     "
     gene            complement(7393..8178)
                     /locus_tag="KPG09_002816"
     CDS             complement(7393..8178)
                     /locus_tag="KPG09_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0967410.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFDIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKEHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            8643..9008
                     /locus_tag="KPG09_002817"
     CDS             8643..9008
                     /locus_tag="KPG09_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0967411.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            9086..9640
                     /locus_tag="KPG09_002818"
     CDS             9086..9640
                     /locus_tag="KPG09_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG0967412.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            9860..10534
                     /locus_tag="KPG09_002819"
     CDS             9860..10534
                     /locus_tag="KPG09_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG0967413.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      10889..10985
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            11039..11215
                     /locus_tag="KPG09_002820"
     CDS             11039..11215
                     /locus_tag="KPG09_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967414.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(11403..11642)
                     /locus_tag="KPG09_002821"
     CDS             complement(11403..11642)
                     /locus_tag="KPG09_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967415.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(11787..12596)
                     /locus_tag="KPG09_002822"
     CDS             complement(11787..12596)
                     /locus_tag="KPG09_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0967416.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
BASE COUNT         4371 a         1981 c         1424 g         4863 t
ORIGIN      
        1 tttttattat acaccaatag cttttttagc taatttatca gcttcctcat tatacttatt
       61 tccagaatga gctttaacct ttgtaaattt tacattaagc ttattcttaa tactgtcata
      121 gaaattttta tatgctatag ttccttcttt atttgttttc caaactccag tacaccactt
      181 ttcaatccct tcatagtcaa agtataaaat caagttttga acattattgt caatacagta
      241 ctgcattgca attttagaag cctctatctc tccagctaca tttctcatac ttactaaagt
      301 tttctctttt cctttttcac tataagtttt tataacttca ttatttttta aaatcacaac
      361 acccgaacca tacattttta ctgaatgttc ataacttcca tctacatatg cttctataaa
      421 ctccttatct tcttgaaaac tcaatttttc tgacccatca atatattctt tagcttcttg
      481 aaaagtagaa aaactcttat attcagctcc tgagaatcca tttacttgtt ttttgcactc
      541 atcccatgta ttatatacac ctatattttt accttttttc acagcataaa acttttgttt
      601 tgccaaatat ctcactccca ctcttaattt ttatcttctt ctctcataaa tatatctaaa
      661 tttataagat agtttctatc aaaatatttt actttcatat ctttttttat aatatcaata
      721 tggtacactt ctccttttac agctctttgt cttatatttt catttatatc tttttttata
      781 atatctttat caacatcatt gttatccatt ttagcatcaa cattcaacgc ttttatagta
      841 tcaaatataa cagattttag tattaaactt ctagatttat ttaaatgtcg ccttatataa
      901 gtataaaact cattgctatt aacctttgaa attttacaat cgtgtaaatc tttctctaat
      961 ctctttaaaa cacttatatt agttcctaca tcaaaaatca caaaacatcc atagtcatat
     1021 ttgcttctta aaattctccc catagcttgc tttaccttta tggtcatttg aggataattt
     1081 atagtatagt agtctatacc atacttcttc attatagtaa aatacaaagg gtcttttgga
     1141 tttagattcg gaagcttgtc taaagttaca caaaccaaac catcccctgg aatgtctacg
     1201 ccttcaaaac atccttttga gccaagtact acacattttc tatttaaatc gttaaggtgt
     1261 ctaatcccct ttttatcagc ataaatttct aagttaaaac tatgtaagta ttttttcaat
     1321 atttcataag ttttttcttg tctatcttta ctattaaaaa gagctaatgt atgaccttct
     1381 gtaatctttg atatattaga tataatttta ctcatctcat ttggaaattc agaatttctg
     1441 tactcacaaa tatcagaaaa tccaactaca gagactctat ttttataatc aaaaattggt
     1501 tctataatct tttccacatt cttaactctg tctattccta atgtattttt aaaataagac
     1561 atactttcag ataaactcaa agttgctgat aaaaatattc ctttttctaa ttgagataaa
     1621 atattttctt caaataaatc tgctattttt aatggtacta ctcgaaattc aaagtcatta
     1681 taattcttat caatttcaac tatccttgca taatcatcct tttcatcata ctctaaaaag
     1741 atttcaaata taatctttat atcctctaaa tctcttgtct ttgcctttcc aaatttatat
     1801 atatctgctt ctttatctat actatcatca tcaatattcc tataaattat tattattatt
     1861 gaaactaaat tttttattat tttctcacaa ctcaacttta tcttttcact ataggctcta
     1921 taacttattt cagtatctat accatccttt tttagttttc ccacaatttc atctatttgc
     1981 aaattcagtt cccatcttaa attataatta cttacattat tatattcatt acaattacca
     2041 aactttaata ttgaatctat ctcttcaata atgagattta tacttctagc aattttttgt
     2101 ttatcttctc ttccaatttt taatacatta taaaatttat caaaagcctt tatttttctc
     2161 atatttctac tatatttttt atatataaag ctactatttt gaataaactc gtaaggataa
     2221 atttcttgta ataaatacct taaagacttt gaatttataa tgcttgaaaa gaaatcatat
     2281 cctttttcag tcaaattatg tgcttcatct actattatat tttctaaagg tttttcatcc
     2341 ttatacggcc atttagctaa tagtgaatga tttactactg ttatatgttc ttcttttaat
     2401 tcttctattc tgtttttata taaacaatct tttttgcatt ttttaggtct gcacatattt
     2461 gggtcacaac ttacatttct taagtgggta cttatttcct tgaagttgtc aaacactaaa
     2521 taatttattt cttctatatc tccatacttg ccaccttcaa ctaatctctt caaaaatatt
     2581 aaagataaaa tttctccttt agtagggttt tgagactcat aatcatctat atatgcttcc
     2641 agtctatcta tacaaatata gttatttttt ccctttatat atccgtaact tacttttcca
     2701 tttagaccca aagaatctaa cacatttgga atatctttat ttattaattg tatctgcaat
     2761 tcctttgtgt cagtagaaat taataatctt tttttattaa ttctagcctc taatattgca
     2821 ggcaataaat atcctacact tttacctata ccagttggtg cctctataca tgcaattttt
     2881 tcttcatctt cgctatttct aaacaactct cttattgttt tagtaagctc atattgacca
     2941 ggtctatatt catatgtaaa tccttcttta ttctcccaaa tagttttgta ttttaataat
     3001 tcttcataat ttttttcttc ttcatttaaa atttttaagg tttctctttc cttcttcttt
     3061 tcttcttttg agtcaaatat attatattct ttttttatat ttatattatt tgataaatca
     3121 taatttgcct cttctaaaaa tttgctccat tcccatgttg gaagattaaa ttttttaaga
     3181 tatgaattta ttttaaaact caatggctcc aaagtagttt tttcatagtt attaaatttt
     3241 actaaaaggc aatttacaat atttattata tcaatagcat ctgataaagc tctattttcc
     3301 actttagaat tacaatttgt taaagtattt tttaagtatt ctaagctata atctttatga
     3361 tatggttcta aaataactgc taattcaatt aaatcaataa atttgttttt taactttggc
     3421 atatgatact caaaaaactt ttttttcaga cttaaatcat gacatataat gaaactatct
     3481 tctacaaaat ttacaagtct atcttccacc agttctattt cattagctat ttctaaatca
     3541 tttttatcta aatttttaca tactgagaat acttctatag gtacctcaaa tttgtttttt
     3601 attaatgatt cataggtata tattttccag tctttaacct tgacagctcc aacttctaaa
     3661 atttctgaat ttaaacaatc taatccacta acttctatat ctaaaaaaac aacatcattc
     3721 aatacactta ttatattatc catttttata tcccttcaag ttaaaccagc ctaacttatt
     3781 tacaatcatt tctctttata ttttatcatt tatatacact ttatttaaga taattaatat
     3841 caactttata tcttacagta aacttctttc atttattatt tgtatatgtg aatattttta
     3901 actataaata ttcaaactat attataagaa atatacttta aattttaaat aagaggtgaa
     3961 attgtgaaaa aagatagcct aaaaaaatac attatgatag gagcatttgc tctaatactt
     4021 tttggaattg ctagtataaa ttttaaatca ttagataaaa caaaaactca aatttctagt
     4081 ccaacactcg acacacatga atatgattgg tattttaacc ctagggagga tggtaaacag
     4141 ccatcaccta taaaagaagc tgattttttt aaaaaatacg gagcttatta tgtaggaaat
     4201 cccaatgaaa aagttatata tctatcattt gatgctggat atgaaagtgg aaacactcca
     4261 aaattattag atacgcttaa aaaacacaat gctaaagctc aatttttcgt agttgaaagt
     4321 tatataaaaa gcaatccaga gttaataaag cgtatggaaa aagaaggaca tttagtttgt
     4381 aatcattcaa aaagccatcc atcaatggca ggtattacag attttgaaaa attcaaagaa
     4441 gaaattacaa gtgtagaaaa agcatacaaa gatgttactg gaaaagaaat gcctaagtat
     4501 tttagacctc caatgggtaa atttagcgaa caatctttaa aatatacaca agatttagga
     4561 tataaatcaa tattttggag ttttgcttat gtagattggt atgaaaagaa acaacctact
     4621 catgaatttg ccaaaaacaa aatttattca agaactcatc ctggtgcaat agttttactg
     4681 catccaaatt catcaacaaa tactgagata ttagatgagg ttcttacaca ttgggaaaaa
     4741 gaaggttata aacttaaaac acttgattat ttaaataata aaaaatagta tcataaattt
     4801 taaaaaactc tatcatgaat catagtaaaa atatagttat ttataaatac tttaattctc
     4861 gatagagttt tttatttttc attgcctaaa ataggatttt taaatacttg taatcactaa
     4921 gctttcttgt taaaaactgt ataatatgct aagctatgaa caaggagttg acagctatga
     4981 ctaccatttg tgtaatgatt gaccatatat ttttagatac agagtataga caaccaacta
     5041 tccataaaca tcttgcaaaa catattatta tatcacagtc tggtaactta tctgtgacgt
     5101 ttgataaggg cgaatcatta caatgtaaag gaattattat tgattccaat gtatcgcata
     5161 cggtgtcaag taaagataat aaaatgttgg ttttcttgat agatgataca agcatactcg
     5221 cccaaaatat tagtcaacac caattactaa atcactcttt tttcgttata gataatctta
     5281 tagttgaacg aatacaaaat atttatacag cttgtcaggt aaaaggattg tcccaaagct
     5341 atattgattt ttcaaaacaa gctttccgtc tattgaattt aaattatcat atttcgtcta
     5401 ttacagacag tagaatacaa tgggcattga atcatattct aaagtccaca cacattcaca
     5461 atgaaatctt ttttgagata tgtgccgaag taaaattgtc caaaagccgg ttttctcatc
     5521 ttttcaaaga acaaatggga gtatcactaa acagctattt ggctttatca aaacttcgca
     5581 aggcttatga gttattgtta aaaagtggag atattacctc agctgctatg gacgcagggt
     5641 ttagttcacc gtctcatttc tcagcttcca gcaaaaaata tcttggcatt actgcaagtg
     5701 agcttatagg aaaatgtaaa atatatttta tccgccaata gcgtctattt gaaagtcagt
     5761 acttctaata tttgttacaa taaatgcata gcattaaaga ttactcttta ttgctatgca
     5821 ttatcttttt aggagtgatg acattgaaaa caaataaaat ttcttttaat aaaggtctat
     5881 attaccttaa tacaaatgcc aattttaatt ttcagttaaa caggattatt atgtgggatg
     5941 gggggcgctt ggaagatata caaaaagttt cctctaaaat tgtcgatagc gaaagttgga
     6001 aaacagaact tatgtatctg gcacaagaag ctgaacaaga tggacgcaca gaaaatgcaa
     6061 ttgcttacta tcgtatgagt gaatttttta tgtatgatgg tgaccctgat aaaatatcag
     6121 tctacaaaaa agcaactact ctattctaca actactactc tgaatatttc tcaaatggaa
     6181 ctgtacaaat ttttcatgta ccatatgaaa atatcaccct acctgtaatg tatgcaaaag
     6241 cgcaaggcga ggtaaaagat gttatattgc tccacggtgg aaatgattct tactttgaag
     6301 aattcttttt cccaatgctt tatcttgcgt cacagggatt tgatatttat ctttttgagg
     6361 gacctggtca aggtggagtg atgcgtttgc agggaaaaca ctttacttat caatgggaac
     6421 gtcccgttaa gacaatttta gattattttc atttagagaa tataactatt gtaggtgctt
     6481 ccttgggagg ttttcttgct ccacgagcag cagccttcga aaaacgaatt gaacgcgtgg
     6541 tagcttggtc tgtattcccc aatttcttag acataatagt tggttcacaa ccaataaaaa
     6601 tgcaaaatct acttagacta ctgctaaaac tcaaaacaaa atggctagtc aattttataa
     6661 taaagcaaaa aatgaaaagt ggcgatagta tggtggtatg gggtttaaaa catggaatgt
     6721 acgcatatga agcgtctaca ccctatgaat attttcagaa aatcaagtat tatcaaatag
     6781 attctattgc aaaaaatata aaccaagaca ttttgatttt aggtgcaaca caagatcatt
     6841 ttattgatta tagaactgtt ggtaaagaga ttgatttact caacaatgtc cgatccctta
     6901 catttaggct tatgacagaa gcagaacaag ctagtaacca ctgtaactgt ggaaatagca
     6961 aactggtttt agatacaatt tcaaattgga ttatgatgat gaaaatacat gatagagtct
     7021 aaatattgat ttttaaatta tacaaaatcc atctgctagc atatccttat tgctttgttc
     7081 aaataatcac ttgttaaaaa attggttccc acttacgaaa tactacgttt atagaaatga
     7141 actatatcat gtaagcacac aatatatatg ataataagac ccaattactc aatgtaactg
     7201 ggtctttctt atgtcataga ttttatagga ctcatgaaat gtaaaagagt atttcaccga
     7261 tttccaaagg gaactttact tagataagga gaccataaat gctaacaaac ccatctacta
     7321 gacacctttc aagtatttga gcgattatac aatacacgtt aagaattaca cgaaaattta
     7381 ttatttttat agctatttaa aaagaattgt ccattcattt atctctttgt atcctatatt
     7441 atgatatatt ttacctgcta catcattatt ataaaaaaga catggaactt ttccttccaa
     7501 caataattct ttactcaaat tgtaaaccat ataacttgca agtccttttc ccctatatct
     7561 tttatctgta cttacagaga ccaccattgc caaaaaacta gtttccatac cagttgacac
     7621 agtgctaatc atagtatcat catttctaat aaaatatgct cttacatttc cctcttttaa
     7681 atgttctttc ctttcttcta tgtaattttt acttacttta tagtctgacc tatttaaaag
     7741 ttttcctatt tcatcaatat ctttttcctg agcattttct attttatact ccttcatgtt
     7801 ggaaaaatct atttctttta tttcttttaa tacacaaaat ttattatcta gtttttcata
     7861 aaaatctttg tatttactta ttagtctatc tattacacat ttttttccac ttatgctttc
     7921 tacatctaaa tcttttatat gattcttcat ttctcctata tcaaaattat ttttaaaact
     7981 ataaattata agtgttgact tattttttaa tattatagag gttatatttc catcttctac
     8041 ctgagaccat atatcttgat attgagagct aaatccataa ttctcaacgt atccaattat
     8101 gaataaattt aaactttctt catgtgatac atattgccaa aattcttttt ctaaagtatt
     8161 atctattttt acaatcaagc agatatcctc cttctatgtt aataatgata taccttataa
     8221 tttatagtat catttaaaat cattatattc taccaaatat tctaaatttt taaatatcta
     8281 tgcaatatgt tcgtatttat ttttatattc tgttactgct tcactccaag cttcttctac
     8341 tgaaatacct ctcttatttg caattttttg tgctaattct attattttca tagcttttct
     8401 tgatatattc atatatattc ctcctctaaa ttatcataag ttattttaaa gttaaatttc
     8461 tatagtcatg ttatatattt atttacttat atcttactaa ttatttatgt actaaatatg
     8521 aactacaata gaagaaattt agaacttaat aaaacataaa atttatatac agttcatata
     8581 tatttgtcat atcacaagac aagtgatata atttattatc attttactgg gggtgatatg
     8641 ctatgaaaat aagagctgaa tatacatgtc ctcttgaaat tgtacatgac ataatcaaag
     8701 gaaaatggaa aactatcctt atatttgaat taagacaatc taataaaaca ttttctgaat
     8761 tgcaacataa tatttatgga ataaatcaaa aaatgttgat tcaacaatta aaagagttac
     8821 gtgattttgg gattattgat aaaataagtt ctgctggata tccattacat gttgaatatt
     8881 ttcttactaa tcgtggtaaa aaaatgttaa aagcagtgga aataatgcaa gaaataggaa
     8941 tcgaatatat gcttgaacat ggtcaacaag aaattctaga taataaagga atttgctaca
     9001 ataagtaatc ctatacatac caaaaagtaa gtaatttaca ttttttacaa tagtattata
     9061 ataaaagtaa atagaaggta taatcatgtc tatagcttta atattgattg atattcaaaa
     9121 tgattatttt aaaaatggaa aatgtgaatt atttcaatca gaagaaactg ctgaaaatgc
     9181 taaaaaaata ctactgtttt ttagaaaaaa caatttacca gttattcata tacagcatat
     9241 atcaacagat aaaacagctt ctttttttct tcctaatact catggttcag aaataaataa
     9301 aattgttttt ccactagata atgaagaaat tataattaaa cacacaccag atagtttctt
     9361 tgaaacagat ttgcaaagta tccttgagaa aaaaaatatt actaatctag taatctgtgg
     9421 aatgatgagc catatgtgta ttgatacaag tgtaagaaca gcaaagaaac ttcgatacga
     9481 cataactcta ataagcgatg catgtacaac gaagaattta tcttggaata atatagaaat
     9541 taatgcaaat actgtacatc aagtattcat ggcatcacta cagaacagtt ttgcagatgt
     9601 aaaaaacaca gataaatttt tatctaacta tcacaaataa aaacatacac tatgttaaaa
     9661 gagcaagttt gatttattta agcttgctct ttaatcattt aaaattagat gctttctatc
     9721 atttttatat acataatata cgtttataca ttttatattc atatataact attatataaa
     9781 ttttaaactt tactatattc acataaaaaa tgtagtatta tagtatatat aatattatat
     9841 agaagggggt gctagataaa tgaaccatca tatattgatt caatttgaat atatagcaag
     9901 aatcattact gctggtttct gtggggcttt aattggatat gagagaaaga atagactaaa
     9961 agaagctgga atacgcactc attttatagt ggcattagct tcagcactta taatcattgt
    10021 ttccaagtat ggatttaatg atatagtggg tactccaggt attggtctag acccagcccg
    10081 cgttgcagca caagttgtca gtggtatagg ttttttaggt gctggattaa tatttattag
    10141 aaatcaatca gtaagtggtc ttactacagc tgctggtata tgggcaacag ccggtacagg
    10201 tcttgccatt ggctctggat tatacttagt tggtattgtt tcaagtatca ttattgttat
    10261 tgtacagata acacttcata gagataggaa gtggatgaaa ctccctacag cagagcaagt
    10321 ttctcttaaa atttctaaca gcactgattc catagaatat attgaaggta aatttaaaaa
    10381 atctaatatt gaaattataa acatgaatat taaacgcctt gatggggatt ggttagaagt
    10441 agttatatat actaagttac ctaaaggata taaaaaaaca aatttattaa aattatttag
    10501 cgataatcct cttatagaaa ctttagaaat ataatataaa aattgaagct taaaaataat
    10561 aatatattta atcaaggtca atatataaat aattacggga tgagtctata taaaattcat
    10621 cccttgtttt ttgccattca tatatatttt ttcaagatat ttttgctcat ctattatcta
    10681 gccaagtaac tcttgtgaac taaacttttt aatacaaaaa cttcaataca aaactaaata
    10741 taatttttat taaatattct acatgtatag tttgtttctt gagtgaaaaa tagatttcat
    10801 attgttaaga tactgttaaa tatttcaatt ttctgacaag tatgttgaac aataacttat
    10861 cctagtgtat tattttagtt gtagaataaa actaaatcgg caaaactaga gaaatttagt
    10921 gacgcaaagc tatagggact aacgcttata aaataaacta tgagttatgt cagccagttg
    10981 ccaaaaagat attgttcttt ttgttttatg aaagtttttt agggggatag taatatatat
    11041 gtttagagat gaaatggata aatgtacaca catgttaact gcttatatta gtagtttata
    11101 tgattattgt gattttatag atacacagct agatgatttt atactagagt acggagaaaa
    11161 tgtagtagaa tcttgtttac atcaagtgat ggtattggta agtaagtata attaaataga
    11221 aattgttttc tagagaaccc tttttagttt tatctacatt taaaaatgtt attatattaa
    11281 aaaactaatc tcccctatat gcaaagtttt cttccatata acaatttctt cccatttata
    11341 ctttttcact tgtatcttta ataaaattca acttcaaata ttaatatact tcaaaaatta
    11401 ttctatttct tatctttaca ccaattccta tcagtattat cctttatata ggttctaact
    11461 atttcgccac aatctaaaca taaatctgaa tatatagtag attgtactgg aaaagtttta
    11521 tttataactt tatctaattt tccatctcct ctatatttta atccaatagt ttcagcatat
    11581 atgccaccag ttgttaaaat acccttttca atatttacac ttccacaatt agaacatttc
    11641 acatttataa ccccctaaac aattattttt tcatgctatc ttataattta aaatataatt
    11701 tgctaccgat tttataacac agtagcaaat cggtggcaaa tcaacatttt ttcattctat
    11761 ttaccatcta atccattgaa atatggctat ttagttatag gaacttgaat ttccgttaca
    11821 aactcttcta caattccagt ctcatgtata tctatcttat agatttctat agggtcagca
    11881 attatcttat aattattttc ttctataaat ttaaacacct tctccatata catcttatta
    11941 ttagaattac tcccagtata atttaaagtt acataatatc cttcatcaaa aactatatta
    12001 tatactttct cattactttc cagaaaacaa aatacagatt taaactcatt aaatatacct
    12061 ttattaattg cttcagtaga aaaggcagaa ccaatattgt tgtttccaag tatattaaat
    12121 ctatcttcat attctttctg aagtttctga attaaaaaat cgaagtcttc atccctcttt
    12181 atatcagcat ttaattctaa agcttttctt ttttctatat atacaacctc tatctcatct
    12241 atttttgtat tttgaattac actttcaatc gaactcaatc tttttataat attttcctta
    12301 tggcttataa attcagctat ttttttatca attaaatcga gttcttcatt taaaatattt
    12361 ttcgtagact ctatacttct gtcatctagg tattctttta ttttcttcat tggaaagttc
    12421 aagcttctaa attcttttat taaatttaat ttccatatat ctgatatatt atacattcta
    12481 tatccattta tatctctaac tggtcttaga atgcctattt cttcataata cattaatgaa
    12541 tctctaccta ttccatatat tttagatatc tctcctattt tatagtaatc tttcatttat
    12601 ataataaact cctccgaaat cctattttta ttttaaatc
//
LOCUS       DAELSY010000116        12603 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.116, whole genome shotgun sequence.
ACCESSION   DAELSY010000116 DAELSY010000000
VERSION     DAELSY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12603
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.116"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            128..2473
                     /gene="secA"
                     /locus_tag="KPG09_002823"
     CDS             128..2473
                     /gene="secA"
                     /locus_tag="KPG09_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG0967417.1"
                     /translation="MSVLDAILDKADEQEIKKLNLIVDKIESLEKDMEIKENEQLKEM
                     TNTFRLRLDKGESLDDILPEAFAVVREVSKRVLGMRQYKVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMRPVYEFLGLTVGVILSNQD
                     PSIRKEQYKCDITYGTNSEFGFDYLRDNMVPDLSHKVQRELNFAIVDEIDSILIDEAR
                     TPLIIAGDGDEDLKLYELANNFVKTVKEEDFEMDRKDKTIALTASGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKNESKTMATVTYQNFFRLYEKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNRPVIRADLHDKVFKTEEEKYSAVVEEIIRIHKTRQPILVGTVSVEKSEKLSKMLKK
                     QGIKHQVLNAKQHDKEAEVISKAGKLDAITIATNMAGRGTDISLGAGDKEEEEKVKNL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIKEDIQKMVKDIIQEAGETYLIGRKRDYYGYFKHLYSTFMPADTLLIPGVDKKS
                     VQEIIDSTYEISKRVYDLKKMMIGIDKVAELEKTVLLKVVDQYWIDHIDAMEQLRQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
     gene            2888..4759
                     /locus_tag="KPG09_002824"
     CDS             2888..4759
                     /locus_tag="KPG09_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HBG0967418.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRDLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTDTVTSKTIEIGIGDEKIDLTANPVDSTGTNLDPSTEGFRVNKIVK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISS
                     KDSETGKYKFTIKYTDASGKAIELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADVDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKADITDSQRNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            4866..5573
                     /locus_tag="KPG09_002825"
     CDS             4866..5573
                     /locus_tag="KPG09_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG0967419.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAREIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYKEGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDELAINKIQYVHAP"
     gene            5606..7441
                     /locus_tag="KPG09_002826"
     CDS             5606..7441
                     /locus_tag="KPG09_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG0967420.1"
                     /translation="MKISKKIVSLLTMTFLTVTLYGNTSNASTKDTLTGSGRWETAIK
                     ISQAGWKKSENAVLVNDNSIADALSATPFAKAKDAPILLTQSNKLDSRTKAELKRLGV
                     KNVYLIGGSIALSSEIEKQLNAENISFERISGNSRYDTSLKLAEKLDREKSISKIVVV
                     NGEKGLADAVSVGAIAAQENMPIILSDSENGTEVADNFIDSKDIEKSYVIGGTYSISN
                     SVERSLPNATRIAGSSRSETNAKIIEEFYKDTDIKNIYVTKDGTRSKHDLIDSLAVGV
                     LASKNGSPILLAGNKLDSSQKDVLNTKIIDKVIQIGGLGNENVVEDVIDMQEETKYTV
                     ETIDELNVAIKKSDANDIIRFKPEKDKNITNSFKIETNKAITIEFDGIYKQSITIDMP
                     NGDIKNFGDIDNDLVINNIKNETLVNEGSIQGIDVYDENGCKIENEDSGEIWLITILK
                     NSKDVYILNRGEITKISNNSSSVTIKNSGNINTISGSEEPSISGKKPITNDTDKDEDK
                     KARGLYPKVESCIPALKDYVRITISTKPESSKYKIYYRVVDEKPSAKYVGEKISTKGW
                     SMVTISNQSFIERAKDGSYIEAVEIDTSNNEVSRWGKSSITDDGI"
     gene            complement(7489..7884)
                     /locus_tag="KPG09_002827"
     CDS             complement(7489..7884)
                     /locus_tag="KPG09_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBG0967421.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIEIRFVFKEFLSFVSFRLLSGVVEMLIMYVMIDLLF
                     VNDVIVKIFTNIVVIVLNYLFSKMIIFKK"
     gene            8111..10522
                     /locus_tag="KPG09_002828"
     CDS             8111..10522
                     /locus_tag="KPG09_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HBG0967422.1"
                     /translation="MRKYKSKKLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMDLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISIGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNESAFNELN
                     TLLGK"
     gene            10819..12396
                     /locus_tag="KPG09_002829"
     CDS             10819..12396
                     /locus_tag="KPG09_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBG0967423.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVENLEAKVDSIEKAIDSISQKVN
                     KIQDILDKIGFLKKFLS"
BASE COUNT         5249 a         1408 c         2286 g         3660 t
ORIGIN      
        1 acttaaatat ataaagatgt aatttattta agtatgatta ttatactata caataaaata
       61 attgtctact attctataat tttaaggtat aataataatt gtatgataaa agaggaggtc
      121 aaaaaatatg tcagttttag atgcaatctt agataaagcg gatgaacaag aaattaaaaa
      181 attaaattta atagtagata aaatagaatc tttagaaaaa gatatggaaa tcaaagagaa
      241 tgaacaatta aaagaaatga caaatacttt tagacttaga ttagacaaag gtgaaagttt
      301 ggatgatata cttccagaag catttgctgt tgttagagag gtctcaaaaa gagttttagg
      361 aatgcgtcaa tataaagttc agttaattgg aggtatagta attcatcaag gaaagatagc
      421 tgagatgaag actggtgagg ggaaaacttt agttgaggta gctccagtat atctaaatgc
      481 tttaacagga aaaggtgtac atgtaattac agtaaatgat tatttggcag aacgtgataa
      541 agaacttatg cgtccagtat atgaattttt aggattgact gtaggtgtaa ttttatctaa
      601 ccaagaccca agtataagaa aagaacaata taaatgtgat ataacttatg gaacaaatag
      661 tgaatttgga tttgactatt taagagataa tatggtgcca gatttgtctc ataaagtaca
      721 aagggaacta aactttgcca tagtagatga gatagactca atattaatag atgaagctag
      781 aactccactt attattgcag gagatggtga tgaagattta aaactttatg agttagcaaa
      841 taactttgta aaaactgtta aagaagaaga ctttgagatg gatagaaaag ataaaactat
      901 agcattaaca gcaagtggta taagcaaagc tgagtcattt tttggtataa caaaccttac
      961 tgatataaag aatatagaat tatatcatca tataaatcaa gctttaagag gtcataagct
     1021 tatggaaaaa gatgttgact atgttatctc aaatggagaa gtaatgatag ttgacgaatt
     1081 tacaggaaga gtaatggatg gtagaagata tacagatgga cttcaccaag ctatagaagc
     1141 caaagaaggt gttgagataa agaatgaatc taaaactatg gctactgtga cttatcaaaa
     1201 tttcttcaga ctatatgaaa aactttctgg tatgactggt actgcaaaga cagaagaagg
     1261 ggaatttgag tcaatctata aattaaatgt tgtccaaata ccaactaata gaccagttat
     1321 tagagctgat ttgcatgata aggtatttaa aacagaagaa gaaaagtata gtgctgttgt
     1381 agaagaaata ataaggatac ataagactag acagccaata ctcgtgggaa cagtttctgt
     1441 tgaaaaatct gagaagttat ctaaaatgct taagaagcaa ggtattaagc atcaagtctt
     1501 aaatgctaaa caacatgata aagaggcaga ggtaatttct aaagctggta aattagatgc
     1561 tataacgatt gctacaaata tggcaggtag aggtacggat atttctctag gtgcaggaga
     1621 taaagaagaa gaagaaaaag ttaaaaattt aggtggtctt tatgttatag gaacagaaag
     1681 acacgaatca agaagaattg acaatcagct tagagggcgt tctggtcgtc aaggagaccc
     1741 aggtacatca agattttttg taagtcttga agatgatgta ataaagcttt atggtggaaa
     1801 aactatagag aaacttatga agagaacaag ttcaaaggaa aatacggcta ttgaaagtaa
     1861 agcacttaca agggctatag aaagagcaca aaaaggtgta gaaggtaaga attttgaaat
     1921 aagaaaaaat gttcttaaat atgatgatac tattaatgag caaagaaagg ttatatataa
     1981 tgaaagaaat aaagtgttaa atgatgaaga tataaaagaa gatattcaaa aaatggttaa
     2041 agacatcata caagaagcag gagaaactta tttaattgga agaaaaagag attattatgg
     2101 atattttaaa catttatata gtacatttat gcctgcagat acattactaa tacccggtgt
     2161 agataaaaaa agtgtccaag agataattga tagcacatat gaaatttcaa aaagggttta
     2221 tgacttgaaa aagatgatga ttggtattga taaagttgcg gagttagaaa aaacagtact
     2281 tttaaaagtg gttgatcaat actggataga tcatatagat gctatggaac agttaagaca
     2341 gtatataggt cttaaatctt atgctcaaaa agatccgttt aaagaatatg ctttagaagg
     2401 atatgatatg tttgaagctt taaataaaaa tataagggaa gcaacagtac aatacttata
     2461 taaatttaac taaaaaagtt catgtaattt ttattaaatg aaaaataaaa tattttgatt
     2521 ctttatattg taattacaat taaataatta aggaatacaa atgaaaaaga agctgaaaat
     2581 cagcttcttt ttctgtgtga gaaatacgga aaaaagagat gcttgaaatt tctttcttcc
     2641 atttaatatt atactataaa gaagatgaaa agtctattag ttataaactg gattattaaa
     2701 aaaaattaca attttgttac ttttttgtat acataaatat taaaaaaata aaatataaga
     2761 aaaagtttat atcttttggt taattattac aataagtctc atttattgaa ataatatcaa
     2821 atatatatta taattggtaa ataaggaaaa aataataaaa atttgaattt tttaggggga
     2881 aaataccatg aataaaaaaa atctttctgt aattatggct gctgcaatga taagtacatc
     2941 agtagctcca gtttttgctg cagaaactac acaggtaaaa aaagaaacaa taactaagaa
     3001 agaagctaca gaactagttt cgaaagttag agatttaatg tctcaaaagt atactggtgg
     3061 ttctcaagtt ggacaaccaa tatatgaaat aaaagttggc gagactttat caaaattaaa
     3121 aataataact aatatagatg aattagagaa attagtaaat gctttgggag aaaataaaga
     3181 acttattgta actataacag ataaagggca tataacaaat agtgcaaatg aagtagttgc
     3241 agaagcaact gaaaaatatg aaaattcagc agacctttcc gctgaggcta attctataac
     3301 agaaaaagct aaaactgaaa ctaatggaat ttataaagtt gcagatgtaa aagcttcata
     3361 tgatagtgct aaagataagt tagttataac tttaagagat aaaacagaca cagtaacttc
     3421 taaaactata gagataggta ttggtgatga aaaaattgat ttaacagcaa atccagttga
     3481 ttcaacggga acaaacttag acccttctac agaaggattt agagtaaata aaatcgttaa
     3541 actaggtgta gcaggagcta aaaatattga tgatgtccaa ttagctgaaa taactataaa
     3601 aaatagtgac ctaaatacag tttcaccaca agatttatat gatggatata gattaactgt
     3661 taaaggtaat atggtagcaa atggaacatc aaagtcaatt agtgatattt catcaaaaga
     3721 ttcagaaaca ggaaagtata aatttactat taagtatact gatgcatctg gaaaagcaat
     3781 agagcttact gtagaaagta ctaatgaaaa agatttaaaa gatgccaaag ctgcattaga
     3841 aggtaattca aaggttaaat tgatagctgg agatgataga tatgcaactg cagtggctat
     3901 agcaaaacaa acaaaatata ctgacaatat agttatagtt aattcaaata aactagttga
     3961 tggattagca gctacaccac ttgctcaatc taaaaaagca cctatattat tagcatccga
     4021 taatgaaata ccaaaagtaa ctttagatta tataaaagat ataattaaga aaagcccatc
     4081 agctaaaata tatatagtag gtggagaatc agcagtatca aatacagcta aaaagcaatt
     4141 agaatcagta actaagaatg ttgaaagact agctggagat gatagacata tgacttctgt
     4201 agcagtagca aaagctatgg ggtcttttaa agatgcattt gtagtaggtg cgaaagggga
     4261 ggctgatgct atgagtatag ctgccaaagc tgctgaactt aaggctccta taatagtaaa
     4321 tggctggaat gatctttcag cagacgctat caaattgatg gatggaaaag agattggtat
     4381 agttggtggt tctaacaatg tatctagtca aattgaaaat caacttgctg atgttgataa
     4441 agatagaaaa gttcaaagag ttgaaggaga aacaagacac gatactaatg ctaaggttat
     4501 agaaacatat tatggaaaat tagataaact atatatagca aaagatggat atggaaataa
     4561 tggtatgcta gtagatgcat tggcagcagg acctctagca gcaggtaaag gtccaatact
     4621 tctagctaaa gctgatataa cagactcaca aaggaatgca cttagtaaaa aattaaatct
     4681 tggtgcagaa gtaactcaaa taggtaatgg agttgaattg acagtaatac aaaagatagc
     4741 taaaatacta ggttggtaat aacttatact gtggagaaga ttccttaatt aatacaggaa
     4801 tctttttcta taattatgct atttattgta aattataaca ttttataaag taggaggatg
     4861 tagtagtggt agatgcaact ttatttttta caccacatca agatgatgaa accttaagta
     4921 tgggaagtgc tataattgaa catgttgaaa aaagtgatac acatgtcata ttatgcacag
     4981 atggtagtaa atctataata agaaaagttt tggatgatgg agggtcatgt tcttatcata
     5041 ttaaagatgt tcataaatac tctttatcag aaagtgaatt ttcaaaagat agagatgaag
     5101 aatttaagga tagctgtgaa gcaatgggtg taaaagaaag taatatacat atagaagata
     5161 atagagcaca tgatggtgag ttaagtaaag aaaaggcaag ggaaataata cttaagtact
     5221 tagaagaata tccagatgca aaagtaaaaa ctgttactcc gtttaaagct agtggaatac
     5281 atgaagacca tagggcatta ggagaagctg cactagagtt atataaagaa ggcaaaataa
     5341 aagatttaag attttatgta gaaccatacg actataagga ttttaaaaaa gttaatccaa
     5401 atgtagaggt atggaaggta ttacctagtc aagaggagaa gttattaagt gcaatgaatg
     5461 catataaaaa atggaatcct gaaagtggac attatgctat aggatatcat tcagtaaaat
     5521 ctcattttga tgagctagct ataaataaaa tacagtatgt acatgctcca taaaatatta
     5581 tgaaagaatt aggaggtaag aattaatgaa aatatcaaaa aagatagtgt ctttgttaac
     5641 tatgacattt ttaactgtta cattatatgg aaatacatct aatgcatcta caaaagatac
     5701 attaacaggt tctggaagat gggaaacagc aataaaaata agtcaagcag gatggaaaaa
     5761 gtctgaaaac gctgtgctag taaatgataa ttcaatagca gatgctttat cagctactcc
     5821 atttgctaaa gcaaaagatg caccaatatt attaactcaa agtaataaat tggatagtag
     5881 aacaaaagca gaattaaaaa gacttggtgt gaaaaatgta tatttaatag gtggttcaat
     5941 tgcattaagt tcagagattg aaaagcagtt aaatgcagaa aatataagtt ttgaaagaat
     6001 atctggaaat agtagatatg atacttcttt aaaattagct gaaaagctag atagggaaaa
     6061 atctatatct aaaatagtag tagtaaatgg agaaaagggt cttgctgatg cagtgagtgt
     6121 tggagctata gctgctcaag aaaacatgcc aataatactt tctgattcag agaatggaac
     6181 tgaagtagct gataatttta tagatagtaa agatatagaa aaatcgtatg taataggtgg
     6241 tacatattct atttctaatt ctgtagaaag aagtttacca aatgcaacaa gaatagcagg
     6301 tagtagtaga agtgagacaa atgcaaaaat tatagaagaa ttttacaaag atactgatat
     6361 aaaaaatatt tatgtaacaa aggatggtac aaggagtaag catgatttaa tagattcttt
     6421 agctgtgggt gtattagctt ctaaaaatgg ttctccaata cttttagcag gaaataagct
     6481 agatagttct caaaaagatg tgttaaacac taaaattata gataaagtta ttcaaattgg
     6541 cggattgggt aatgaaaatg ttgtagaaga tgtaatagat atgcaagaag aaactaagta
     6601 tactgttgaa actattgatg aacttaatgt tgctataaaa aaatcagatg caaatgacat
     6661 cataagattt aagccagaaa aagataaaaa tataactaat tcttttaaaa tagaaacaaa
     6721 taaagctata actattgaat ttgatggtat atataaacaa tcaataacta tagatatgcc
     6781 aaatggagat ataaaaaact ttggggacat agataatgat ctagtaataa ataatataaa
     6841 aaatgagact ttagttaacg aaggaagtat acaagggata gatgtttatg atgagaatgg
     6901 atgtaaaata gagaatgagg attctggaga aatatggctt ataacgatac ttaaaaattc
     6961 aaaagatgta tatattttga atcgtggaga aataactaaa atatctaata attcttctag
     7021 tgtaacaata aaaaattcag gtaatattaa tactataagt ggaagtgaag aaccatcaat
     7081 aagtggtaaa aaacctataa ctaatgatac agataaagat gaagataaaa aggcacgtgg
     7141 tttatatcct aaagtagaat cttgtatacc tgctttaaaa gattatgtaa gaataactat
     7201 atcaacaaaa ccagagagtt caaagtataa aatttattat agagtagttg atgaaaaacc
     7261 atctgcgaaa tatgttggag aaaaaattag tactaaaggt tggagtatgg ttacaatatc
     7321 aaatcaatct tttattgaaa gagccaaaga tggatcatat atagaagcag tagaaataga
     7381 tacctcaaat aatgaagttt cacgatgggg aaaatctagc attacagatg atggaattta
     7441 gacattaaac ttattcattt tgcagattac tatttagtaa tctgctattt attttttaaa
     7501 aattatcatc ttgctaaaca agtaatttaa aactataaca actatattag taaatatttt
     7561 tacaataaca tcatttacaa aaagcaaatc aatcattaca tacataatta acatttcaac
     7621 gacaccagaa agtagcctaa aactaacaaa agacaaaaat tctttaaata cgaaccttat
     7681 ttcaattctt ttactttcaa atacaaaaaa cttattagta acataagcaa ataaaacagc
     7741 aagtatccat gccaaagcat ttgcaaccat aaaattaaat aaaataactc tagtaaaaaa
     7801 taaatacgaa actatattta caagagtagt aaatgctcca aaaaataaat ataatattgt
     7861 ctctctatgc ttttttaaaa tcaaatataa tcctcctaat ctaatttaat ataaatatac
     7921 ttaaagatac taacaaacat tagtatagca catctattaa taaatcaata tatcttaaaa
     7981 tattataaaa ataaaactat aatattctat aattaatatg tactcataat ccatatttta
     8041 tcaaaataaa atatgacctt aatttagaaa tctgatataa taaaaattaa atacatataa
     8101 gggggtaaac atgagaaagt acaaatcaaa aaaattgtca aagctactgg cattgttgac
     8161 agtttgcttt ttaatagtgt caacaatacc tgtctcagca gaaaaccata aaactctaga
     8221 tggagtagaa actgcagagt attctgaaag ttatcttcaa tacctagaag atgtcaaaaa
     8281 tggagacaca gcaaaatata atggagtaat accttttcca catgaaatgg aaggtacaac
     8341 acttcgaaat aagggcagaa gtagtcttcc atcagcatat aaatcaagtg tagcatacaa
     8401 cccaatggat cttggtctta ctacaccagc aaaaaatcaa ggaagtctta atacatgctg
     8461 gtctttttca ggtatgtcaa ctttagaagc atatcttaaa ttaaagggat atggaacgta
     8521 tgacttatca gaagaacatt taagatggtg ggcaactggt ggaaaatatg gatggaactt
     8581 agatgatatg tcaggtagtt caaatgtaac agccatagga tatctgacag catgggcagg
     8641 tcctaagtta gaaaaagata taccatataa tcttaaatct gaggcacaag gtgcaactaa
     8701 accttcaaat atggatactg cacctactca atttaatgta acagatgttg ttcgtcttaa
     8761 taaagataag gaaactgtaa aaaatgctat aatgcaatat ggttcagtga catcaggtta
     8821 tgcacattat tcgacatatt ttaataaaga tgaaacagca tataactgta caaataagag
     8881 ggctccatta aatcacgctg tagcgatagt aggatgggat gataattatt caaaagataa
     8941 ctttgcatct gatgtaaaac cagaatcaaa tggagcatgg ttagtaaaaa gcagttgggg
     9001 agaatttaat tctatgaaag gattcttctg gatttcttat gaagataaaa ctctactaac
     9061 agatacagat aactatgcaa tgaaatcagt atcgaaacca gatagtgata aaaaaatgta
     9121 ccaacttgaa tatgctggtc ttagtaagat aatgtcaaat aaagtaacag cagcaaatgt
     9181 atttgatttt agcagagatt ctgaaaaact tgactctgtt atgtttgaaa cagactctgt
     9241 aggagcaaaa tatgaagtat attatgcacc agtagtaaat ggagttcctc aaaacaactc
     9301 aatgacaaaa cttgcaagtg gaacagtatc atattctgga tacataaatg tacctactaa
     9361 ttcttacagc ttaccaaaag gtaaaggagc aatagtagta gttatagaca acacagcaaa
     9421 tcctaataga gaaaaatcaa ctttagcata tgaaactgat atagatggat actatctata
     9481 tgaggctaaa gcaaacttag gtgaaagcta catacttcaa aacaataagt ttgaagatat
     9541 aaatacatat agtgaatttt ctccttgtaa ctttgttata aaagctataa caaaaacatc
     9601 ttctggacaa gctacttcag gagaatcttt aactggagca gatagatatg aaacggcagt
     9661 taaagttagt caaaaaggat ggacttcttc acaaaatgca gtattagtaa atggagacgc
     9721 aatagtggat gctttaacag ctacaccatt tacagcagca atcgactctc caattctttt
     9781 aacaggaaaa gataacttag attcaaaaac taaggcagag ttacaaagat taggaactaa
     9841 aaaagtttat ctaataggtg gagaaaattc tttaagcaag aatgtacaaa ctcaacttag
     9901 taatatgggt atatcagtag aaagaatttc aggtagtgat agatacaaga ctagtatatc
     9961 tctagctcaa aagttaaata gtataaaatc tgtttcacaa gttgcagtgg caaatggtgt
    10021 aaatggactt gcagatgcaa taagtattgg tgcagcagct gctgataaca atatgccaat
    10081 aatacttact aatgaaaaga gtgagttgca aggtgctgat gagtttttaa attcatcaaa
    10141 aataactaag tcttatataa ttggtggtac agctacttta tcatcaaatt tagaaagtaa
    10201 gctttcaaat ccaacaagac ttgcaggaag taatagaaat gaaactaatg ctaagataat
    10261 agataaattc taccctagct cagatttgaa atatgctttt gttgttaaag atggttcaaa
    10321 gagtcaagga gacttgatag atggactagc agtaggagca ttaggtgcta aaactgattc
    10381 accagtagtt ctagttggaa ataagttaga tgaaagtcaa aaaaatgtac ttaagtctaa
    10441 gaaaatagaa actcctatta gagttggtgg aaatggaaat gaaagtgctt ttaatgaact
    10501 aaatactctt ttaggaaaat agtgaatgga aagatagtaa ggatatacat tttattatta
    10561 aaaaatattc aatagaagtt tataaatata aaataacaaa aaggggtgtc tcaaaatgaa
    10621 ctttttagtt catgaggcac tcttttttgt tgttttatat ttttatcatt taaataatga
    10681 aaaataggct tgtctatatt tttagatagt tttcttatta ttgatgaaaa atgtaataat
    10741 tttaaactgt aaaatagtgt aaagttattt tataatataa atatagtaag attttaaaca
    10801 aaataagggg gaataataat gaaagcacca aaaactattt taacaatact gacaatagcg
    10861 cttactttaa gtagcatttc tataatacca tcatatgcac ttacagagga aaaattaata
    10921 ggtaatggta gatatgaaac tgctgtaaag ataagtcaaa aggcatacag ttctagttct
    10981 aatgtagtgt tagttaatga caattcatta gctgatgcac tttcagctac accatttgca
    11041 aaggctaaag gagcaccaat acttttgaca gaaagtgata agctagatga taggactgaa
    11101 aaagagataa aacgtctagg agctaaagat atttatttga taggaggtac tgctgtactt
    11161 aataaagata tagagaacaa gttaaaaggt aatggactta atgtagaaag aattaatggt
    11221 aaggatagat atgaaacttc actgattttg gctaataagc ttaaggatat aaaagatatt
    11281 aaggaagttg ctgtagtaaa tggtgaaaaa ggattaagtg atgctgttag tgttggagca
    11341 ccagcagctc aaaataagat gcctataatt ttgtctagtc ctaaggacgg agtagaagca
    11401 tttgataaat ttataagaga tgagaaagtt ataaaagcat acgtaattgg aggaacaaat
    11461 tctgtttcaa gagctgtaga aaagagcctt cctaatgcag aaagagtgag tggaaaagat
    11521 agaaatgaga ctaatgccaa agttatagaa aagttttata ctgatacaaa tttaagtaat
    11581 ttatatgtaa ctaaggatgg tagtaaaaat gagaatcaac taatagactc gttagctgtt
    11641 ggagtattgg ctgcaaaaaa tgagtctcct gttgttttgg ttggtaataa attaaacacg
    11701 aaacaaaggg atatacttag tactaaaaaa ttaaatacta taacacaggt aggtggaaat
    11761 ggaaatgaag aagcttttga tgaaattaag agtttacaag aaaaaactgt gtttgaagca
    11821 aaaactgttg aggaattaac tgatatgata aatatagcaa gtcctaatga tattataaac
    11881 tttaaaccaa aagagaatac tgtaaatgaa gcttttagaa tggtaacaaa taaaccaata
    11941 actgtgaata taaagggtga ttgttcaaag actcttacag tagatatgcc taatggtgaa
    12001 gtgaataact atgctacttt ggtaaatgtt atagttagaa atattggaga aggtggattt
    12061 aataaccatg atacaatcac tatattgtca gttcgtgaca aaaatggtag agttatagaa
    12121 aatactagaa attcagatat agatacgttg atgatattag cttctgctaa tgatacaaaa
    12181 ttgattaatg atggatatat aggaaagctt atagataatt catctaatag tgatataact
    12241 aataatggta ctatagataa aaaagttaat caagttgaga atttagaggc taaagtagat
    12301 tcaattgaaa aagctataga ttctataagt caaaaagtca ataaaataca agatatacta
    12361 gataagattg gatttctaaa gaaatttctt agttaagaat atgtatattt aagtgaagtg
    12421 tgtgttgtaa ttgttctaaa gttatatagt taaacaggtt tatatacaaa cagacttata
    12481 gataaatatg tagtttagta tatatttgaa taagctttgg ggaggtaaca tggggattgg
    12541 aattgaatgt aacaaaaaaa taaataaaag tattgacaaa actagaataa agaaaaataa
    12601 tct
//
LOCUS       DAELSY010000117        12489 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.117, whole genome shotgun sequence.
ACCESSION   DAELSY010000117 DAELSY010000000
VERSION     DAELSY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12489
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.117"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..744
                     /locus_tag="KPG09_002830"
     CDS             <1..744
                     /locus_tag="KPG09_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBG0967424.1"
                     /translation="NNKEIVDLENLINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHD
                     KYLHTIGNLTLTGYNSKLSNKTFEEKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNR
                     TKILLDRAVEIWKYPSVTYEPPSNSENIFTLEDDDVNFTNESIVSFSILGESYKAKNW
                     TEFYEQVALTLYDLDSVRFNSIVDKTYKRDFIDNIKSRDVYKLRKGIKISENIYLETN
                     LNTEAKLNIIRFLLNEFDIELNEVIFSIK"
     gene            complement(961..1527)
                     /locus_tag="KPG09_002831"
     CDS             complement(961..1527)
                     /locus_tag="KPG09_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0967425.1"
                     /translation="MDTKKVTFSSICIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            1693..2214
                     /locus_tag="KPG09_002832"
     CDS             1693..2214
                     /locus_tag="KPG09_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG0967426.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFNEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
     gene            3005..5083
                     /locus_tag="KPG09_002833"
     CDS             3005..5083
                     /locus_tag="KPG09_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBG0967427.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            5083..5862
                     /locus_tag="KPG09_002834"
     CDS             5083..5862
                     /locus_tag="KPG09_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0967428.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            5855..6664
                     /locus_tag="KPG09_002835"
     CDS             5855..6664
                     /locus_tag="KPG09_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0967429.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            6684..7166
                     /locus_tag="KPG09_002836"
     CDS             6684..7166
                     /locus_tag="KPG09_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0967430.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            7187..7591
                     /locus_tag="KPG09_002837"
     CDS             7187..7591
                     /locus_tag="KPG09_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0967431.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANDDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(7747..8907)
                     /locus_tag="KPG09_002838"
     CDS             complement(7747..8907)
                     /locus_tag="KPG09_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG0967432.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            9115..10665
                     /gene="xylB"
                     /locus_tag="KPG09_002839"
     CDS             9115..10665
                     /gene="xylB"
                     /locus_tag="KPG09_002839"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBG0967433.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNIIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYINTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYKIYHRLYKRIYKSLKDDFKLLDE
                     VVNTKINN"
     gene            10761..12098
                     /gene="xylA"
                     /locus_tag="KPG09_002840"
     CDS             10761..12098
                     /gene="xylA"
                     /locus_tag="KPG09_002840"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBG0967434.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNVTDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
BASE COUNT         4872 a         1400 c         2202 g         4015 t
ORIGIN      
        1 aataataaag aaattgttga tttagaaaat ttaatcaata ataatatgct aaatatagag
       61 catataatgc cacagacatt aacaaataaa tggaaggaat cactaggaga taactataag
      121 gagatacatg ataaatattt acatacaatt ggaaatttaa ctttgacagg atataattca
      181 aaactaagta ataaaacatt tgaagaaaag aaagaaatgg aagatggatt taaagatagt
      241 aggctttatc taaataaata tatttcatct atagataaat ggaatgaaga agaaataaaa
      301 aatagaacta agatattact agatagagct gtagaaattt ggaaatatcc aagtgttaca
      361 tatgaacctc caagtaacag tgaaaatata tttacgttag aggatgatga tgttaatttt
      421 acaaatgaaa gtatagtatc atttagtata ttaggagagt catataaggc taaaaattgg
      481 acagaatttt atgaacaagt tgctcttact ttatatgatt tggattctgt aaggtttaac
      541 agtattgtag acaagactta taaaagagat tttatagata atattaagtc tagagatgta
      601 tataaattaa gaaaaggtat taaaatatca gaaaatatat atttagaaac aaatttaaat
      661 acagaggcta aattaaatat aattagattt ttacttaatg agtttgatat agagttaaat
      721 gaagtaatat tttccataaa ataatgaaat atatgcattt tttgcgtaat agaatattaa
      781 ttatattaaa aataaaatag cacctcttga tatagattta tagctcaaat ttatatcaag
      841 aggttacgtt taattaaaat aatacaaata ctatctaatt aaattattaa taaaaatgtt
      901 atataaaaat gagtatatag cattttaaag agtaataaat cttacataat aaatgattta
      961 ttaatatgaa ctagaagtat ttttactata ttgttttata aaacctttag gcattttact
     1021 tataaaatat accaccataa tacaagacaa aggtttgtct actatattac taagaatcct
     1081 tggaaagaac gcagcagtaa atatcttctg accagactga actaaaaaac caaccaataa
     1141 atcagcacca cttccactta aaccaccaaa caataaaact gaaataggag taccaattaa
     1201 aggacaaaca atagataaaa ttactcctgt aataatagct tctttataac caaaaccttt
     1261 tttcttagaa atatatccaa caataagacc aagaacaata tttacaagag caaaaggtat
     1321 atttgtagga cctgatataa cacctaaaac taagtttgta cacccaccaa taagagcacc
     1381 ccaaaaagga cctaatacga cagccccaag tacagtacca actgtatcta aaaataataa
     1441 tggtatattt atcatagata taaccatacc taagacaata ttaataacta tgcatattga
     1501 agaaaatgtg acttttttag tatccaatta taatacctcc taaaaaaata tatacattta
     1561 tcatagtata gattataaaa gattactaat tgtatatttc tatatagaat attggaaata
     1621 tagaacaaat aaataatata attatcaaaa tatgtagtat cattaaaatt aagaataact
     1681 aaggagatta ttatgaagag aaatagagag attgtagtag tatgtcattg tatattaaat
     1741 tgtaattcta aagtagaggg attgtcagaa tttaagggag cacaaaatat agcgataaat
     1801 ttaataaata aagggtatgg aataattcaa ctaccatgcc cagaaatgat tatgtatgga
     1861 ataaataggt ggggacatgt aaaagaacag tttgacaatt tgttttttag agaacaatgt
     1921 aggcaaatgt taatacctca tgtaaggcag tttaatgaat atttaaaaaa tgattatgat
     1981 ataaaaagta tcattgcaat agatggtagt ccaagttgtg gttataataa gacatgtagt
     2041 tctaaacgtt ggtttggtga gctaagtggt tgcaatgaat tgatggataa aataaatgat
     2101 atacaaatgg tggatgggaa aggtattttt atagaagagc tagaaaagct acttaaagaa
     2161 tataatttag agatacctat agttggattt gacgagctta gagaagataa gtaaagatat
     2221 aaatatttat tgtctcatta tgaaacacca ttatatttgg cagaaaaata tgactgatgg
     2281 acagtacctg aatcattttt aagtggagta attagtactt ataaagatgt atctactagc
     2341 tgaaagaaaa tgtagagagg taataaatga attgataata tatataaagg agtgtctaaa
     2401 agtgaacttt ttagtttatg agacactctt tttttatatt aaatcaagta tattaaatca
     2461 aatatatttt aatcatttta gcaatgaaaa ataaatttat ctatattttg agacaacatc
     2521 ttttttagtt ttatttattt gttgtcgttt ttgacttggt atacaattat atattttctt
     2581 aaataatttc ataaattatt tataattata aaatccattg ttttgaacaa tatctaatat
     2641 attattatct gtatttataa ggtcactata catattttca agtccaatat tatctacata
     2701 ataggcaaat tttattcctg tatgtgttta aaaaaaacta gatgtatata ggtatcatca
     2761 cagatattaa aagtataaat aaaagatatt aagaacgact ttcaagcaac atattagaaa
     2821 attttactaa aatccggaaa gcgattgaca aacaaagtca aataaagtat attattggta
     2881 agttagttta atgtacaaat gaattatata taaaacctaa tttataaata acgatactaa
     2941 acgacattat ttaacttaag ctacacaaag atagaatatt ataccattga tttaggaggt
     3001 agccgtgata aaaatatctg aagactatat tgaaaaaaga tttgacaatg aactattgcg
     3061 aatagaagca tggggaaaaa actctttacg tattaggtcg tttgtagacc aaaattttgt
     3121 tgatgaaaac tatgctctta acgaaaaacc taagttaaat aaagatgata ttacaattaa
     3181 taaaaatgaa gatggaagtg caatcattaa aaatggaaag ataaaagctg tcttagacca
     3241 tagagataga attacttttt ataatgataa aaatgaaata ttattgaaag aatatattcg
     3301 attaagagct gtaaaacatg atgatggtgg agaagatgta gggacaattg agattaccaa
     3361 agactttaac tctactctaa agttaaaatc aagagaatat cgaccaagct atagtggaga
     3421 atttgaagta actactagat ttgaatctga gccttctgaa aagatttttg gaatgggaca
     3481 atatcaacat aaatttttag atttaaagaa tactgttttg gagttagccc aaagaaattc
     3541 tcaaatatca gttccttttt atatttctag tctaggttat ggacttttat ggaataatcc
     3601 gggaattgga aaagtatctt ttgctaaaaa tatgacagaa tggaagatgt tttctacaaa
     3661 ttttatagat tattggatta cttgtggaga aagcccaaaa gagttaaata aaaattattc
     3721 tcaagttact ggaactgttc ctatgatgcc tgaaaacctt ttaggactat ggcaaagtaa
     3781 actaagatat agaacttcag aagaagtttt agatgttgtg aaggaatatt ctaaaagagg
     3841 aattaagctt tcatcaattg caattgacta ttttcattgg cctaagcaag gtgaatataa
     3901 atttgactta gattattgga aaaatcctaa agaattagtt aaaaaattga aagaagagta
     3961 ttcagtagaa ccaatagtat ctgtatggcc tacagttcaa agtgatgcag aaaactacaa
     4021 tgattattta gaaaatggct atttagtcaa tgttaatcgt ggtgttagaa tgacaatgca
     4081 aattcaagga aatacagtct ttgtagatat gacaaatgaa aatgcaagag aatatgtttg
     4141 ggatagaata gataaaaatt ataaacaact tgggattgac tattattggt tagatgtagc
     4201 tgaacctggc tattctgtgt atgattttga taactataga tataaaaaag gaaatgtttt
     4261 aagttgtggt aatatttatc ctattgatta tttaaaaatg atttatgatg gtttacataa
     4321 tgacatagag tctgttgtaa ctcttgtaag aggagcttgg gcaggagctc aaaagtatgg
     4381 agctttagta tggtctggtg acattgattc tagttttgag gcttttaata atcaagtaaa
     4441 tacagggtta aatatgggac tggcaggtat accatggtgg actactgata ttggaggttt
     4501 ccatggagga aatcctaaag acccagaatt tagagaactt atggtaagat ggttccaata
     4561 tgctactttt tcaccaattt tgagaatgca tggagataga ttgcctcata gtaaaccatt
     4621 atcaaataaa ggtggaggct caatggtaac tggagctcct aatgaaatat ggtcttatgg
     4681 tgaagaagtt gaagtaattc ttacaaaatt cataaaaatt agagagagct taaaaactta
     4741 tttaaaaaaa ctgatgaaag aagcgcatga agatggaaca ccagttatga gaactttatt
     4801 ttatgaattt cctgaagatg ataaaacttg ggaggtggat aatacatata tgcttggaga
     4861 tgaaattttg gtagctccaa taatgaatta taaagataga agtagaaaag tatatttacc
     4921 aaaaggacat acatgggaaa atatatttag tggagttagt tatgaaggtg ggaaaactta
     4981 tgaagttgaa tgcccgctgg aagaaattcc tattttccta aagcaagatt ccagttataa
     5041 ttttaaagaa ctaaaaaata ttttgggaga ggtgaaatca taatggagcc acaattatgg
     5101 caattattat taatcgtact atatggattt tttataaact atgaaaaaaa ttcaacaatg
     5161 tttggaacat atcaaccagt aacagcaggt tttattactg gtcttatctt aggagatata
     5221 aatacagggt tatatattgg aggtacatta cagctattat cattaggtat tagtaacttt
     5281 ggtggagcat caatacctga ttatcaaaca gcaagtatag tagccacatt tataactatt
     5341 acgactaagc aagaagcatc tgtaggaatc tctataggta ttccagttgc tttattgatg
     5401 gtacaattag atgttcttag aaatactatt ggtatatggc tagttcataa agcagaagat
     5461 ggagctaaaa aaggaaatta taaaaatata acctatatgc aaatgttagg agtactatta
     5521 acagcagcta caacaggtat tccagttgct ctatctgtaa tatttggacc ttctttaatt
     5581 aataccattt tgaaatatac acctgaatgg ttaacaggtg gtttaacagt agctggagga
     5641 ctacttccag cagtagggat aggattatta cttagatatc ttcctgctaa ggagtatttt
     5701 agttacttag taattggttt tgttttagca gtatatatga aagtaccatt attaggtgta
     5761 gctttaattg gtggagcaat tgcacttata atttacaaaa agaatttgga aaatcaagaa
     5821 caacaatata ctgtagtagg aggtatggac gaagatgagt gataaggtat cattaaataa
     5881 atcaaatgaa aaggtgaaaa aaagtgtctt atggcgctgg ttttttacta gttctgtttc
     5941 ttcaaattat gagaagatgc aggccttggc atactgttat gcagtactac cttttttaaa
     6001 agtagtatac aaagataatc cagaaaagtt acaaagtgct gtattaaatc atttacaatt
     6061 ttttaacact aatccttggg ttgcccctta tattttgggt attaatgtag caatggagga
     6121 gttatcaagt gaagagactg aagaggctgt tgcttcaatt aaaacaggct tgatgggacc
     6181 agtagcaggt ttaggagata gtttatttgt cgttattccg tggacaattt ttggagctat
     6241 agcagctaat atggcgatag atggaagtcc atttggaatc ttactgtgga ttgtagtgag
     6301 tgtagcattg aaattattaa gtataccact atttaatgct ggctatacat caggtactaa
     6361 gctaatatct tcaattgaaa aaagtttaaa gattttaact gagagtacat ctattttagg
     6421 tttaatggtt gtaggtgcat taataccttc agttgtaaaa gctaatgttg ctttgaactt
     6481 tcaacaaggt gattttacaa tgaaaggtca agaaatatta gaccaaatta tgcctgggtt
     6541 agttcctgca attttagttg tgattgttta ttgggctctt aaaaagaata taaaacctat
     6601 atacttaatt ttaggagtaa tggttgtttc aataatttta tatgcattag gtattttaaa
     6661 ataaggaaag gatgatatta gatatggaaa tgaaaggtat taaaaatatt cgtatagatg
     6721 accgtttaat ccatggtcaa gttgcaacta tgtggagtaa taaattgggt gttacaagat
     6781 taatggtagt aaatgatgca gtagctaaca attctgttca aaaacaagtt ttaagaatgg
     6841 ctacacctgc aggtatagcg tcatctataa ttacagaaga gactgctata aaaaatatta
     6901 ctgcaggaaa gtatgaagga caaaatgtat tattaattgt taagtctcct gtggacctta
     6961 ttccatttgt agaggcagga tttaaaatac ataatataaa tgtaggaaat atgtcaagta
     7021 gaaaagatac aacagttcta agacctaata tcagtgttac tgaagaagag aaagaagcat
     7081 ttaaaagact tttagatgaa ggaattgaaa ttacaactat aatgacgcct gatgataaga
     7141 aaacttatct tgtagacatc ttatagattt aaagaggtgt attaaagtga atagtattat
     7201 tttgatgagt catggcaata tggctgaaga agtcttaaat tctgcaaaaa tgataatagg
     7261 ggattctata gattatccta ctgtaaatat gaaacaagat gatggaatag aaggaacaat
     7321 agaaaaatta aaaaaagctt tagaagtgta caaggaaagt tcagaaataa ttataatggc
     7381 agatttacta ggtggaactc catgcaatgc tgctttatta gtagccaatg atgacagtcg
     7441 aattaaaatt atttcagggc taaatttagg tatggttatt gaatcagctt ttttagatac
     7501 aaaggaattg gcagcaaaat tagagaaaat aggtaaagat aatatttcta tggttgaaca
     7561 aaatgcaagt ttattatctg atgatgagta ggaagattat attaatattt aaaacaaaat
     7621 acttgtgtta atacttaatt gatatacaat ttttaaaaaa tatagctaat cttgcataaa
     7681 ctatataata aatctaattt tagaatgtat aatttaaagg gttatggctg tatatagtca
     7741 taacccttat atttcattaa ttaaagttaa attttgtata ttcaaaaagc tcttgatatt
     7801 tacggcagag ccaccatata gagatgcctc agaccctaat gatgaaattt ctatatttat
     7861 tcctttgtta aaagagctaa ctaacatatc ttttgtaatc tgtaatatct cagggatatc
     7921 actaattatt tggctattta aatatattat ttcaggagca taagttgtaa tagcattatt
     7981 tatagctatt gttaagtaac tacaaaactc atgaataact tttttggcat tctggttatc
     8041 ttcataatat agttgcttta ctatatcaga atctatttta ggaatatttt ctaaagaaga
     8101 aagctgttca aatacctttt tttctgaaca atactgttct aaacagccac gatttccaca
     8161 aggacatagt ttaccattag gcatgatgat agtgtgacca atttctccac tcataccatt
     8221 tctgccgcta tataatttat tatttattat aatgcctgaa ccaaatccac tgtgaatact
     8281 gagactaagt agtgagttat gtacagttga aaaagtattc tcagctaaag ctgttaagtt
     8341 tgcttcattt tctatatgaa taggaaagtc atactttttg ctaaggatgc tatataagtc
     8401 aatctcatta agattataat atggagtaaa caatacttta ttttcacaag taatcccatg
     8461 tatagccaaa gtaagaccaa ttaccttata aggagtgtct attttggata tgttataact
     8521 atttataatt tcatctatca attgtataac attgtcttta cttacttgta tatctgttaa
     8581 tttcttagaa tttataatag ttccatctaa gtaagataga gaagaaaata tatagtcgta
     8641 tccaatgtcc atacttaaag aaatccctgc acatttatta aatactaata atatgggttt
     8701 tcttccgcca ctatgagtac tatttccaat tcctatttca tgaactagag attcatcaat
     8761 aagttttttg gtaatagcgg atatagttgc tttattcaat cctatagtag aagctatact
     8821 tgccctagaa ataggaccat tttttataat ttgttcaagt accaatcttt cattcatttc
     8881 tcgaatagtg tatttatcag taaccaattt gatattcctc cttaaaataa tattgtaata
     8941 ctttttacac aaaaataaaa ggttattttg cattgacaaa gataattaaa tattttatta
     9001 ttagttcata agttagttta atatactaac aaaaataaag caagtaaaat atacctaaaa
     9061 tataaaaaaa ttaggatagg aaaacgatag ttatgaagtg gcattcaagg agggatgaag
     9121 ttggagtatg tattaggtgt ggatattggt acatcaggta ctaaaacagt tttatttgat
     9181 aaactaggaa atattataaa aagttgtaca tatgaatatc ctcttataca agagaaatca
     9241 ggttgggcag agcaggatgc aaatgattgg tggaaagctg tagtcgaatc tataagagaa
     9301 gtagttcaaa gttcaaatat aagcagtgaa tgtataaaag gaattggatt atcagggcag
     9361 atgcatggtc tagttatgct ggacaatgaa ggtaaaacat tgagaaactc tataatatgg
     9421 tgtgaccaac gaacagtaaa agagtgtgag gagataactg atttagtagg tgaagaaaga
     9481 ctgattgaaa taactgctaa tccagcgctt acaggattta cagcatcaaa gatattatgg
     9541 gttagaaaca atgaaccaga tatatatata aatactaata aaatacttct tccaaaagat
     9601 tatataagat acaaattgac aggtgaatat gctacagagg tatcagatgc aagtggtatg
     9661 cagttgctcg atataagaaa aagagattgg agcgatgaag tactagaaaa gttaaatata
     9721 gataaaaatc ttttaggtaa agtttatgaa tcacaagaga ttacaggtta tgtgactaga
     9781 gatgtagcaa gtttgacagg gcttaaagaa ggaacaatag tggtaggagg agctggagac
     9841 caagcagcag gagctatagg aaatggaata gttaaagatg gagtagtttc atctactata
     9901 ggtacatcag gtgtagtatt tgcttatacg aaagagccaa aaattgataa agaaggtaga
     9961 gttcatactt tctgtcatgc aataccaaat acatggcatg ttatgggagt tactcaagga
    10021 gctggattgt cattaaaatg gtttaaagat aatttctgtc aaagtgaagt ggaagtttca
    10081 aattctttag gtgaagatgt atatgagatt ataaataatc aagtaagtca agtaccaaca
    10141 ggatgtaatg gattgcttta cttaccatat atgatgggag aaagaactcc tcatcttgac
    10201 ccttatgcaa gaggtgtgtt ctttggatta tctccaatcc attctaaaaa agaaatcgca
    10261 aggtcaataa tggaaggtgt ttcatatagt cttaaagact gtatggatat aatagagaat
    10321 cttaatatag acgttaatga agtaagagcc tcaggtggag gtggaaaaag taaagtatgg
    10381 agacagatgc aagcagatat gtttaatcaa gatgtatata ctataaattc aagtgaagga
    10441 ccagcacttg gagttgcaat tcttgcatta gtaggagctg gaatatatga aaatatacaa
    10501 aaagcttgtg atgctataat aaaaacttca actaaattag aaccaataag tgaaaatgta
    10561 gatacataca agatatatca tagattgtat aaaagaatat acaaatcttt gaaagatgac
    10621 tttaagttat tagatgaagt agttaataca aaaataaata attaaattag cttaaaaaga
    10681 aaaataaaga tagaaaatag ttttaaatta agaaaacata cgacatatat tatatgtgta
    10741 aaatattagg aggacatgaa atgagtgaaa tatttaaagg aataggacag ataaaatttg
    10801 aaggagtaaa atctgacaat gaattagcat ttagatacta caatcctgaa caagtggtag
    10861 gtaataagac tatgaaagaa cacttaagat ttgcaatgag ctattggcat actttatgtg
    10921 gagagggtaa tgacccattt ggagtaggca cagtagagag accttggaat aatgtaacag
    10981 acccaattga gatagcaaag attaaagttg atgcaggatt tgaatttatg tctaagatgg
    11041 gaattgagta tttctgtttt catgacagag atatagctcc agaaggaaga gatttagaag
    11101 agacaaataa gatattagat gaaatagttg agtatataaa agttaatatg gaaaagacag
    11161 gtataaagtt attatgggga actgccaata tgtttggaaa tccaagattt gttcatggtg
    11221 catctacaac atgtaatgca gatgtatatg catatgcagc agctcaggtt aaaaaagcta
    11281 tggaaataac taagtactta ggaggagaaa attttgtatt ctggggtgga agagaaggtt
    11341 atgaaacttt acttaacact aatacagaac ttgaaatgga taactttgca agattcttac
    11401 aaatggctgt agattatgct aaggaaatag gattcactgg ccaattctta atagagccta
    11461 agccaaaaga gcctactaaa catcaatacg attttgatac tgctactgtt ttaggatttt
    11521 taagaaagta taatctggat aaatacttca aaatgaatat agaagcaaac catgcaacac
    11581 ttgcaggaca tactttccaa catgaattaa atatagcaag aataaacaat gttttaggta
    11641 gtatagatgc aaatcaagga gacctattat taggttggga tacagaccaa tttccaacaa
    11701 atatatatga tgcaacactt gctatgtatg aagtgttaaa gcaaggtgga atagcaccag
    11761 gaggatttaa ctttgactct aaagtaagaa gagcatcttt tgaggtagaa gacttattcc
    11821 tagcttatat tgcaggaatg gatacatttg caaagggatt attaatagct cataaattat
    11881 tagaagatga ggtatttgaa aactttacta aagaaagata tgcaagtttt agtgaaggta
    11941 taggtaagga tatagtagaa ggtaaggttg gatttaaaga gttagaatct tatgcactgc
    12001 aaatgccagt tataaagaat aagtctggaa gacaagaaat gctagaagct atacttaaca
    12061 gatatatata tgaagtagat acaatttcta ataagtaatt gtgtagtaat taaacttgga
    12121 tgtttgaata aaataacagg atatgttctt gtttttattc taaaacggac aaagataagt
    12181 atttgtaaaa attctcataa tcatgataaa ttaaggatat agacttaagt ataattagtt
    12241 tatatcctta aaatttctat aaaaaataat catttctata gttatcaata tttttaatta
    12301 tcaatacctt tctcaactaa taaataaaaa tcagtaaagt atctaaattt aagtcattat
    12361 aatggataaa ttttttagca tatctttttt agtatatcac tgataagaat ttactttcag
    12421 gatatagcag taaaatagat atttgaatat aattgttatt ttataacttt catgaaataa
    12481 ttttgaaaa
//
LOCUS       DAELSY010000118        12267 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.118, whole genome shotgun sequence.
ACCESSION   DAELSY010000118 DAELSY010000000
VERSION     DAELSY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12267
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.118"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            247..354
                     /locus_tag="KPG09_002841"
     CDS             247..354
                     /locus_tag="KPG09_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0967435.1"
                     /translation="MGRHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            356..1475
                     /locus_tag="KPG09_002842"
                     /pseudo
     CDS             356..1475
                     /locus_tag="KPG09_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1593..2135
                     /locus_tag="KPG09_002843"
                     /pseudo
     CDS             1593..2135
                     /locus_tag="KPG09_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            2280..2942
                     /locus_tag="KPG09_002844"
     CDS             2280..2942
                     /locus_tag="KPG09_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0967436.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCGNISLNKVEVKAYLDNVEVLLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            2942..4123
                     /locus_tag="KPG09_002845"
     CDS             2942..4123
                     /locus_tag="KPG09_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0967437.1"
                     /translation="MKTPRNINSTVVFIIFCLIITFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(4304..6964)
                     /locus_tag="KPG09_002846"
     CDS             complement(4304..6964)
                     /locus_tag="KPG09_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0967438.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAQKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDRTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKMS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKTVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNTRSRRQEFAMLRSVGLSPE
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(6961..7644)
                     /locus_tag="KPG09_002847"
     CDS             complement(6961..7644)
                     /locus_tag="KPG09_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967439.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(7988..8926)
                     /gene="blaCDD"
                     /locus_tag="KPG09_002848"
     CDS             complement(7988..8926)
                     /gene="blaCDD"
                     /locus_tag="KPG09_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG0967440.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            complement(8954..9670)
                     /locus_tag="KPG09_002849"
     CDS             complement(8954..9670)
                     /locus_tag="KPG09_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967441.1"
                     /translation="MILIVILSIFIIASINIIILGKENIRDIVIPLTLFIVSDFLFLC
                     GNIICRGGVMLLFIGLFAIPLMLIAFIWICVHMIKLYYKKDLNKRKMIGCIFSIFISI
                     GTIFIPSPSESFRFKLYEKDYSVVAKAILKVYDEKKLSSESVFYIQSDKNELEKIFSK
                     EVIKKMKKLNRNLDVVKISGNRNLVYFFFGAELQSIDGIVIFKDLKHVKPKLDFINRS
                     IQAPRLEYITNNVYYFKGEL"
     gene            10002..12245
                     /locus_tag="KPG09_002850"
     CDS             10002..12245
                     /locus_tag="KPG09_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG0967442.1"
                     /translation="MSNIIIKGLTQNNLKNISFKIPKNKIVVFTGVSGSGKSSIVFDT
                     IAAESQRQMNETYSSFIRNRLPKYRKPQVELIDNLTASVIVDQTPLGGNARSTVGTIS
                     DLYSSLRLLFSRIGEPYIGTTSYFSFNDPNGMCKNCSGIGRVKVINIESIINPENSWN
                     TGCINDSLFKPNSWYWKQYAESGLFDLDKPIKDYSFEEYNLLLYGSKNKDGKQENPKI
                     EGIFNKYNQTYLNRDISNMSKHTKEKSERLITDKECPVCHGKRLNKETLNCNILGYSI
                     ADMCNMELTDLYDLLLQIKDKRTETVIKTLTDGLKRMIDIGLPYLHLNRESSSLSGGE
                     AQRLKLVRYMGSSLTGMTYIFDEPSAGMHPRDVHRMNQLLKKLRDRGNTVLVVEHDKD
                     VISIADEVIDIGPSAGTQGGHVVFQGSYDKLLLANTLTSNAMNLSLPIKTDSRHPKGT
                     LPIRDACINNLKHIDVDLPVGIMTVVTGVAGSGKSTLISRVFAQKYEKDVIKIDQSPV
                     TATNRSMPATFLGFFDEIRKVFAKENNVEEGLFSFNSKGACPVCDGKGVLVTELVFMD
                     PIINTCEACKGTRYSEEVLSKLYKGKCIVDVLSMTVDESLEFFTEKKLCKILQTMKDV
                     GLPYMTLGQPLSTLSGGERQRIKLAKNLNKKGTIYILDEPTTGLHGSDIEKLMLIFEH
                     IVDKGNTVIIIEHNLDVLKQADWVIDIGPDGGKNGGQVVFEGTPLEMFERGCTITSDF
                     LRKSINI"
BASE COUNT         4413 a         1808 c         1716 g         4330 t
ORIGIN      
        1 aaagtgtaag aagttcataa gagaactgca taggtagtag cgaacttatg tgaacgacaa
       61 cctcaaaaat gataaaagtt aagacttacg gttcttatat atgttattta aaaataaata
      121 taaaaaacct tcaaaatact tgttttaagt aattgcatat tttaatatta tgataggtga
      181 tgaaaaatgg aagttactca tggtagagga tatgcatata gtatgtcaaa tacagacatc
      241 cgtattatgg ggagacatct ttggaatcct agttatttcg ttgctactgt atcagaaaat
      301 acagaggagc agataagaaa atatattaat aatcagaaaa aacaagtaaa gtgaggtggt
      361 agtagtggaa aaagcttata agtttagaat gtatccaaat aaaaaacaac aagagttaat
      421 taacaaaact tttggatgtt gtagatttgt atataataag tatcttgcta aaagaataga
      481 agtttataaa aacgataaag aaacttttac ttataaacag tgtagttctg atttgaccaa
      541 cttaaaaaaa gaattaaagt ggcttaaaga acctgacaaa ttttcactac aaaacgcctt
      601 aaaagattta gaaaacgcat atgaaaaatt ctttaaagaa aaggcaggat ttcctaaatt
      661 taaatcaaag aaaattaata gattttcata taaaactaac tttacaaatg ggaacattat
      721 gtattgtggt caacatataa aactacctaa acttggtatg gttaagataa gggataaaca
      781 agtacctcaa ggtagaatac ttaatgctac tatatctaaa gaaccaagtg gtagatatta
      841 tgtatcatta tgctgtactg atgtagatat tgaagtattt gaaaatacta acaatcagac
      901 aggtttagat ttaggaatca aagaattttg tatctcaagt tgtggagaat ttatagaaaa
      961 tcctaagtat cttaagaagt cattaaacaa acttgctaaa ttacaaagag aattatcgag
     1021 aaagacaatc ggtagtttaa atagaaataa agcaagatta aaagttgcaa gacttcaaga
     1081 acatatagct aatcaaagaa atgattttct acaaaaatta tctactaaat taataaaaga
     1141 gaatgatatt atttgcatag aagatttaca agtaaagaat atgattaaaa atcataaact
     1201 atctcgctta atttctgatg tatcatggtc agaatttatt cgtcaattag aatacaaggc
     1261 taattggtat ggtagacaaa ttgtataaag taggtaaatt ctttgcaagt tcacaaatat
     1321 gcaataaatg tgggtataaa aacgaggaag ttaagaactt aaatataaga gaatggattt
     1381 gtcctagttg taatgaaact cacgatagag atataaatgc aagtataaat atattaaaag
     1441 aaggattaag actaataacg attcaaaata aataacttat gtaagaaccg taggaactac
     1501 ggggatggcc tgtggagaat tagtaagaca tattttagta tgcaaaattc tatgaagcag
     1561 gaaccctgtg actttagtca tgggaggttc agaatacaac tatgaatgag ttggcaaaag
     1621 aagttgaata tactaaacgc accatataca agtacttttc atgtaaagaa gatttattct
     1681 ttgctgttgt cttaagagga tataaaagac tttgggataa tgttaaaata gaaagtgcaa
     1741 aaggcaaaac agggtttgaa aaaataaaac tttcatactt tgcttttcat aaattttact
     1801 ctgtagaacc ttctctatta tgccttatgg gaatgattgg tattgtaaac tctgaaaaat
     1861 cggatactga tatgctgttt aaagaaaagt ttttttcttt taataaattt atgtttgatg
     1921 aaatacaagg aatgtttgaa attggaaaaa atgatagaag tattaggcat gatattgaaa
     1981 tacctactct catgtattca tcaatattta cacttacagg attttttaat ttactatctg
     2041 taacaggaaa gtcatacctg aacaacttta atatagatga ggaaaaattt attgaaacaa
     2101 ctcttgctct tttaatagat tcaattaaag cataaaccta agtttataaa ttaaagttta
     2161 tatacttaaa tgataatttt ttctatctct aacttctatc tctaacataa aaaatattaa
     2221 acataaaact aataacttag ttaatgaaat aactaaatta ctgaaaggac taaaagtaaa
     2281 tggctaaaat acttctttta gaagatgatg atacaattac atttggaata aaaacttccc
     2341 ttgaaaagaa aaatcacact gtatgttgtt ataatacgat tgctgaagct aaaaaaaatt
     2401 ttgataaaac tattgattta attctgcttg atttaaatct acctgatggt attggatacg
     2461 agttttgcca atatgttaaa agtatagaag atactccagt gatatttttg acagtccgtg
     2521 atgatgaaaa agacattata cgtggattag atatgggtgc agatgactat gtggttaaac
     2581 catttctgct ctcagtactt caatccagaa ttaatgcagt tcttagaaga actcattcaa
     2641 atactaacaa tactattaca tgtggaaata taagcttgaa taaagttgaa gtcaaagcat
     2701 atttagataa tgttgaagta cttttgacag caggagaata taaacttctt ctaagtctta
     2761 tggaaaataa aaatagagct atgacccgta caaagttact agaaatgctc tgggatgtag
     2821 atggtaattt cgtaaatgat aacactctta ctgttacaat aaagcgtctt agagaaaaat
     2881 taaaaaatcc accttgtata aaaacattaa gaggcatagg atatagaatg gaggacagct
     2941 agtgaaaact ccaagaaata tcaacagtac agttgttttt ataatattct gcttaattat
     3001 aactttttta ttaagtgtaa ttctaacaac tataacttct tttaatgaat acaatgtact
     3061 gtctaaggct cttggtgcat catatgagta caatcctaag tctacagaaa gtatactaaa
     3121 atcactgaaa aattctagtg ataaggattt taaaacagga aatgaaattt tatcaaaaca
     3181 tggatattct aaaagtacat tttggagtaa aaactttttt tattttctag tgatatcgtt
     3241 tggtataatt tcaacaatgg ctacagttgt atatataatc aaacatagaa caaataagca
     3301 aaaactgagt agaataaaca atcttactca atacttagaa gaagtgaatt taggaaaaga
     3361 gtctgtactt ttacgatgtg aagatgaatt ttctcacctt gaagatgaga tatataaaac
     3421 agttggggaa cttcgttgtt caaaagaatt tgctttaaaa gaaagacaaa ctctttcaga
     3481 taatcttgca gatattgctc atcaacttaa gactccaatt acttctatgt cacttatggc
     3541 acaattactt tcagaaaatt gtccagatga agaaatagat tatattaaca gacttacaaa
     3601 tcaaatctca agacttgaga gactagtttc atctttactt acattatcaa aattggatgc
     3661 aagtacttta atatttgaaa gcaaacttat agatgtacat tctcttctta cttatgcaat
     3721 tgagcctata gaaggttctt taagagaaaa acatcagaca tttactatcg aggcttctcc
     3781 ttcggtagaa tttgaaatcg atattaattg gactttagaa gctcttttaa atattcttaa
     3841 aaattgtagt gagcatatag aagatggtgg atatatagtt gcttattata gtcaaaatcc
     3901 tctatatgtt gagattatca tagaagataa tggtaaaggg tttgctcagg aagaacttcc
     3961 atatattttt aatagatttt atcgtggtaa aaatgctagt aaggacagta ttggtatagg
     4021 gcttgctcta tcaaagtcta ttatagaacg tcaaaatggt actctacatg cagaaaattg
     4081 ccataatggt ggtgcaagat tcataattaa attttacaca taaaatgctg gattataggt
     4141 aaaataccta taatccagct atatctatat tctatgttat ctattgtttt agtaatatac
     4201 ttctatttac atttaaggtt ttattaaaca aacaaattaa ctatccctat acttgtaagt
     4261 agtcttaaat cgaaaccttt aaataagttc aaatagcttt tgtttatatt gtttcatttt
     4321 ttatagattc tactatattt tcattcttca actgcttgta tcctgtcata tatgatgcta
     4381 ttacaataaa aagtatcaat atagtaaaac ctattattgt agatataggt gcaaatggta
     4441 aatactctat aaaatatatt tcatttatac gtagaaaagc atacactatt cctatttgca
     4501 caggaatact aagtagtaat ggtgtaattc ctaagaacaa accttccaaa acaagcatct
     4561 tttttatgcc ttctggagat aatccaacag aacgcaacat agcaaactct tgtcgtctac
     4621 ttctagtatt tcctgacact gtagcaagta catttgataa tccaatcata gccaataaac
     4681 ctgtaataaa gcctactatt aaattcatgg tctttattcc actagccttt tgtttttcac
     4741 tttctaaaac atcctcaata ctataatctc ctgaaccata gtctttatta taaatggact
     4801 ctaatttttc tcttactcta ggaatgtcct cacgattatt tactttaaat actcctatca
     4861 gcctatttga acgtaaccta cttacttcat cataattttt agatatttcc tgcaccatag
     4921 acattggcac tatttgcatt aatgtatatt ttgaactagt atctactgtt ttaggaagtg
     4981 tgcttgtaat agctcccact ttcatgttgt aagtataatc ccctttatct tcgtcatata
     5041 ctttttcatt taatttaatg ttatctccta ttgaaagatt taaataatct atatatactt
     5101 tatttctacg tgtactacga ttaatatctt cctccttatt atatacaatt gaggttggat
     5161 tttttgtatc ataaaataac tttgggtcta cagaaatttg cctacaatat tctgtaaaac
     5221 tcttatcatc aagcccaatt atatttgaaa ttatacgaaa cttgccatca cgttctatag
     5281 gagagtattt tttagatgaa actatacttt taaatcctcc atttttcgca agttcttttg
     5341 atgcatcttt ttcagtcagc caagtggaag cagacatttt ttgataatat agtgaacttt
     5401 ttacaccttc tgtattatct aatttttcaa tgaaagcaga ctctacaggt tcactatttt
     5461 ccaaataaac tgctatatct ctttggtcct tataattatc acttccataa acagcttttg
     5521 ctccttcttg atttgcaatt atatgcaaaa aacatgtaag taataaaaaa gataatgtta
     5581 aagatattgt tgcagtacga taagattttt tacgagcacg tagtgcgttt gatgcaagtt
     5641 ctccttctat tccaaatatc tttcctaatg aatatttatg cgactttttg attttaacac
     5701 tatctccttg tcgtattgct tctataggag atatctttga tacttttcgt gctggtataa
     5761 gtgcagaaat ccagactgtt actattgtaa gtaaaactgc tggcagaaaa gcaggaatac
     5821 cataagtaaa taccacttct ggaacatcag tatgataatt aactgaatta atataaacta
     5881 taagcctatt acacaataac catcctaaaa ataaacctat tggcaatggt ataattgtaa
     5941 gcaaaaaacc ttcaaacact acagacattt taatctgttt tggtgatgca ccaacactag
     6001 ataatatccc taattgagta agtctagcat ttgcagatag tgaaaacgcg ttgtaaatca
     6061 ccattacaaa cacagctact gtaaaaattg ctattgtaag atacataata ggagttgaaa
     6121 attttgatat actgctcata gatgctttct gttctgggga aaagactaac atttttaaaa
     6181 gataattact attatattta agattgtatt ttccatattc attagtcttc caccccaatg
     6241 atttagcaag ttttggaagt tccttgtatg tgtctcttat atttttaaat cgcaagtaca
     6301 cagttatact atcattaggt tttatacttg ttctatcaag atatccaaga gctgtatatg
     6361 caggtattac tgatgaagtg gaaacatcta cctttcctac tattgtcaat gtagtctctg
     6421 aaattttttt aaaactttcc cctttcgatt tttgagctat gggttcaaga ggctttccat
     6481 ttaggattct atctcctatt ggaagagtaa ttttatcccc aatttttaca cttggattat
     6541 tctcaaaata ttgttttgat aatgcaattt cattagcatt atttggtcga cgaccctcta
     6601 ttatagtatc cttttctggc atagaattcc aatactctga tgtagcattt ctataaatta
     6661 tataatctct tttctcatca ttaattttgc ctacatacca ttgtcctttt accattacat
     6721 catcaactga agaaaaattt tcaattaatg gtaagtcact tccataagta tagtcaaaca
     6781 attcaccatg ccagttacct tctttctcaa ttgaaagtct tataaaatct gtccacatgg
     6841 tatacaacag tccacacaaa gaagacatca tggttgtagt aagtaatata gctaacatta
     6901 tagctatact actacgttta ttgcgtcgta caaagtctaa ggtgtattct tgaagaatat
     6961 tcatttcttc accacctttt cagagacaat cactccatct tccatagtta caatacgatt
     7021 tgcctctagt gctattttct catcatgagt aataagaagt attgtctgat taaatcgttt
     7081 atttgataag tttaacaagt ctacaatttc ttctgaattt tttctatcaa gattaccagt
     7141 tggctcatct gctaataaaa tagctggtct atagattaaa cttctagcta tagataccct
     7201 ttgttgttgt ccacctgaaa gttgacttgg aagatggttt aatctatcag ataatcccaa
     7261 tatagataca atctcagaga atctatcctc atccaccttt cttttatcaa gtaacatggg
     7321 taataatata ttttttctaa catcaagggt aggtataaga ttataaaatt gataaataag
     7381 acctactttt ctacgtctaa aaactgcaag agcttcttcc tttaaacttg atatttctgt
     7441 atcttcaaca taaatctttc cagatgtagg tctatctact cccccaagca gatgtaacaa
     7501 tgtagatttg ccagaaccag atgctcctac aatggataca aaatcacctt tttgaactga
     7561 taaatttaca ttattaaggg ctgtcactct ggtttgattt gaaccatata tttttgtgag
     7621 attttcacat tttaatattt ccatatacaa tcatctcctt tatggttaaa ttttagcaac
     7681 ctaaaatgac attatagtga catgaataca aatataaatt actattaaaa atattgttta
     7741 gtttttgcac attatttaaa attataatat aaaacaaaaa ctatgcaata tattttattt
     7801 atacttttaa tatattacat agtctttaaa aggtattaac tttaaatcta taggtattca
     7861 atttaataat caatagcaag tattttatgt actaaaggag ttttgctcta tatagactca
     7921 atttagtaaa attatttaat aaatattaca gactataggt aaataatatt tacaaataaa
     7981 attaagttta ttcccttaca ctataatatt tcttaattat attaattgca atttccttta
     8041 cctttggccc tgtaatttct ttattggaat catctgattt aatagcaaaa taataggtgt
     8101 caccttcacg ctctagcata ccaacaaacc atgcatccac acgtttattt ttttcatcaa
     8161 atcctgttcc agtttttcca tatacattta tgtttttatc atttacatct attttcataa
     8221 tatctcttaa aatattaata tgctcttttt taaagtttgt atctccctca aatatttttg
     8281 ccattgtttg cacttgttct tttggagata tttgtaatga agattctaac caaaatccat
     8341 ttaaatgtcc ctttccattt tttaaatctc cttcccattc gctaatatcg caattcccat
     8401 actttaaatc atctaaaatg ttttggacag atttagcatc aactttatct atcaattttt
     8461 tgtagtacca aacacaagac tccttaaatg cctcttctaa actaagattt ttattccaat
     8521 ttttatttgg atactctgta ccatcatatc ccataactga ttcttttgag tttattaccc
     8581 ctttttctaa accaattaaa gttgaaacta ttttaaaagt agaacatggt gaccttcttg
     8641 tttcaattaa ttctttgtta tatatattat attctttatt tttagtatta taaaaaatag
     8701 ctcctccact aataccttca aaacagtcac tatagtcaac catattaatt ttttcttttt
     8761 tgtttttatc attgttttca actttggatt tatttaattc agagtttata tgattcttat
     8821 catgtttttg ttttgtgttg ttatgctttc ttcctacata atatactccc atagctacaa
     8881 caactagaag aataactatc caaataaaat tattttttct tttcatactt tataccatct
     8941 cctctacata agtttatagt tcacctttaa aataatatac attatttgta atatactcca
     9001 atctaggagc ttgaatactt ctatttataa aatctaactt tggtttaaca tgttttaaat
     9061 ctttaaaaat cacaatacca tcaatagatt gtaattcagc tccaaagaaa aaatagacta
     9121 aatttctatt cccagaaatt ttaactacat ctaaatttct gtttagtttt ttcatttttt
     9181 ttattacttc tttagaaaat atcttctcca attcattttt atcagattga atataaaaga
     9241 cagattcaga gcttaacttt ttttcatcat aaactttcaa aatagcttta gctactacac
     9301 tataatcttt ttcataaagt ttaaatctaa aactttcact tggactagga ataaatatag
     9361 tacctatact tataaagata ctaaatatac atccaatcat ttttctttta tttaaatctt
     9421 ttttatagta aagcttaatc atatgtacac aaatccatat aaatgcaatt agcatcaatg
     9481 gtattgcaaa taaacctata aatagtagca ttactcctcc cctacatatt atatttccac
     9541 acaaaaataa aaaatcactt acaataaata gagttaaagg gataactata tctcttatat
     9601 tttctttacc taatataatt atgttgattg aggctatgat gaatatactc aatataacta
     9661 ttagtatcat tatgtcctcc tttcagtttg tgaggtaatt atttattctc tatttaaaac
     9721 ttactattgt aatatttaaa tattacaata gtaagtttta aatgtcaagt cgttttaatt
     9781 gtataattat ctttgtactt atcaaattac gagtatctat tatatacttt tttatctatt
     9841 atcaactatc atttattcag attgtatggg attggttaaa tttagaaata ccttctataa
     9901 catagcagaa tatatcaaga aagtagattt taattttatt ataatatggt tacataatat
     9961 aattgcttta ctaaaatgta ctcagaaagg atttaacatt catgtcaaat attataataa
    10021 aaggacttac acaaaataac ctaaaaaata tatcatttaa aattcctaaa aataaaattg
    10081 ttgtttttac tggagtttct ggttctggaa aatccagtat cgtatttgat acaattgcag
    10141 cagaatcaca aaggcagatg aatgaaacat attcttcatt tatcagaaat aggctaccta
    10201 aatatagaaa acctcaggtg gagctgattg ataatttaac tgcttctgta atcgtagacc
    10261 aaactcctct tggtggcaat gcacgttcta cagttggcac tataagtgat ttgtattcat
    10321 cattaagatt attattttct agaattggag aaccctacat aggaacaact tcatattttt
    10381 cttttaatga cccaaatggt atgtgtaaaa attgttctgg gattggtaga gttaaagtaa
    10441 tcaatattga atctattata aatcctgaaa attcatggaa tacaggatgt ataaatgact
    10501 ctttatttaa acctaattca tggtactgga agcagtatgc agagtctgga ctttttgatt
    10561 tagataaacc tataaaagat tattcttttg aagaatataa tttattgctt tatggttcaa
    10621 aaaataaaga tggaaaacaa gaaaatccaa aaatagaggg tatttttaac aaatacaatc
    10681 aaacatatct aaatcgagat attagcaata tgagcaaaca cacaaaagaa aaatcagaaa
    10741 gacttataac agataaggaa tgtcctgtat gtcatggaaa aagattaaat aaagaaactc
    10801 ttaactgtaa tattttagga tattcgattg cagatatgtg caatatggaa cttactgacc
    10861 tttatgattt attgttacaa ataaaagata aaagaactga aactgtaata aagactctta
    10921 ctgatggttt aaaaagaatg attgatattg gtctccctta cctacatcta aatagagaat
    10981 catcatctct atctggagga gaagctcaaa gattaaaact tgtacgctat atggggagca
    11041 gtctcacagg tatgacttat attttcgatg aacctagtgc tggtatgcat ccacgagatg
    11101 tacatcgtat gaatcagcta ttaaaaaaac ttagagatag aggaaatact gtccttgtag
    11161 tagagcatga taaagatgtt atctccatag cagatgaagt tatagatatt ggcccaagtg
    11221 ctggtactca gggtggacat gttgtattcc aaggctctta tgataaatta cttttagcaa
    11281 acactcttac ttcaaatgca atgaatctat cattacctat aaaaactgac tctcgtcatc
    11341 caaaaggtac ccttccaatt agagatgctt gcataaataa tctaaaacat attgatgtag
    11401 atttaccagt tggaattatg actgtcgtta ctggagtagc aggttctggc aagagtactt
    11461 taatctctcg tgtttttgca caaaaatacg aaaaagatgt tattaaaatc gaccaaagcc
    11521 cagttacagc tacaaatcgt tctatgcctg ctacatttct tggtttcttt gatgagataa
    11581 gaaaagtatt tgctaaagaa aataatgttg aagaaggttt attcagcttt aattcaaagg
    11641 gagcttgtcc tgtttgtgat ggtaaaggag tacttgttac agagcttgta ttcatggacc
    11701 ctattattaa tacatgtgaa gcttgcaaag gtactagata tagtgaagaa gttttgtcaa
    11761 aattatataa aggtaaatgt atagttgatg tactatctat gacagtagat gaatctcttg
    11821 aattttttac agaaaagaag ctatgtaaaa tcttacaaac aatgaaagat gttggtttac
    11881 cttatatgac cttaggtcaa ccacttagca cactatctgg aggagaacgt caacgaatta
    11941 agttagctaa aaacctcaac aaaaaaggta ctatatatat tttagatgag ccaactacag
    12001 ggcttcatgg ttctgatatt gaaaagctta tgttgatttt tgagcatata gttgataaag
    12061 gtaatactgt aattataatt gaacataatt tggatgtatt gaaacaagca gactgggtaa
    12121 ttgatattgg tcctgatggt ggcaaaaacg gtggtcaggt cgtatttgaa ggtacaccac
    12181 tagaaatgtt tgaaagaggt tgcactataa catcagattt ccttagaaaa tcaataaata
    12241 tatagataat ataatactaa tattaaa
//
LOCUS       DAELSY010000119        12249 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.119, whole genome shotgun sequence.
ACCESSION   DAELSY010000119 DAELSY010000000
VERSION     DAELSY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.119"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            294..1586
                     /gene="brnQ"
                     /locus_tag="KPG09_002851"
     CDS             294..1586
                     /gene="brnQ"
                     /locus_tag="KPG09_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG0967443.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLI
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIKFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
     gene            2151..4340
                     /locus_tag="KPG09_002852"
     CDS             2151..4340
                     /locus_tag="KPG09_002852"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBG0967444.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEEFIDIKTDLEKVTKANISIRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
     gene            4408..5175
                     /locus_tag="KPG09_002853"
     CDS             4408..5175
                     /locus_tag="KPG09_002853"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBG0967445.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            5233..6249
                     /locus_tag="KPG09_002854"
     CDS             5233..6249
                     /locus_tag="KPG09_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG0967446.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVYAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            6315..6563
                     /locus_tag="KPG09_002855"
     CDS             6315..6563
                     /locus_tag="KPG09_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967447.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELGVLLEEKKELEEE"
     gene            complement(6740..7237)
                     /locus_tag="KPG09_002856"
     CDS             complement(6740..7237)
                     /locus_tag="KPG09_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG0967448.1"
                     /translation="MYKIGESVMYPKEGACYISGLVTKDINHHIQKYYELTVIYNSNL
                     KISIPVLNADKIGVRPIMDENEVENFIQSLDKVDCLWVFDRKKRLKLYHDKFHSGDVF
                     EIVKLIKMLMIQDNSKQLCSTDKDFLNKAQRFALSELAAAQCKSYTIVLEEMKNHILN
                     SKNNN"
     gene            complement(7478..8542)
                     /locus_tag="KPG09_002857"
     CDS             complement(7478..8542)
                     /locus_tag="KPG09_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0967449.1"
                     /translation="MIRLTIANIKRYIKNHALLVNMVLFPVILMFYLNFFTGNSENQS
                     MYFSPVAIVSNSSGKYEHKLIDSSKLKENSFGLNEQDKAMDLLKNNKVSAVFILDKNF
                     SSNIDNLKRPEVKCFKTENGGGSLWAESQIESFITNSLKLKIDRNIDDKLTKTNIIDN
                     SSGKNKNSFLIVFLICYFMYINAAHLASDLFSLKKSNTLKRLVSTKHNDIKIIFSIFL
                     GLFFTQAIVYTLTLVCFTSISNFKLTLNTLMIVLANSFVATGFVFWIARVFKNEGSIS
                     LMSTFYSLIGLAISISNMIPSLDKLSFMANLSKLTPFYWTIDAINNNGNILINIIVLI
                     LIGMVFVTAGSLNLRDFARN"
     gene            complement(8545..9642)
                     /locus_tag="KPG09_002858"
     CDS             complement(8545..9642)
                     /locus_tag="KPG09_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0967450.1"
                     /translation="MKLYISVKTMLKVLKSSFMLNLIYFLALPLVLAGFLGMVTESIF
                     QNPIKTDLTSIVIYDKDNTKLSSNLTGYLKNDLSSILTVQKDDSKADLKLTIPKGYEN
                     NLLNEKSNNLSIEKLKSNDNIAILLQDILDTYHEKLYLNNSQKLSSEDFSKLFNKNSI
                     DTSIIKNNVKQNSYEYFALVSLGFFAIIFIMNSILSNYVSESKGLSKRLYSIPVTRVQ
                     FLIYDFIGFWIYSFIFLSLYVLFFRTLGVTFKGNFVILALLCALASYFMTSISTFITT
                     FFSKKYGTVIVYALLFLQTVFGGIFSIVNDVFTKFTSLSPTYLIGTLFSNYETFKTID
                     SIESLIFTCLIASTVLIALSIVKEKYKWREV"
     gene            complement(9656..10591)
                     /locus_tag="KPG09_002859"
     CDS             complement(9656..10591)
                     /locus_tag="KPG09_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967451.1"
                     /translation="MDIVRVNNITKRFNDKLALDNISFSVKEGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGDIQINGLDLSKDCIKAKSIIGLVPQELALIETLTPFDNLEYFGT
                     YYGLKGKLLQERITEALEVTGLTDVKKKKIKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKNISKKQKTTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVVSILENNSLLELGVDKKLQLTDV
                     LSVVDKNNSKITRISYEEPSLEDVFLNLTGKNLRD"
     gene            complement(10779..11423)
                     /locus_tag="KPG09_002860"
     CDS             complement(10779..11423)
                     /locus_tag="KPG09_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0967452.1"
                     /translation="MNKIKVLIVDDEKLIRKGLNIILSSYDDLEIVGDASNGYEALEF
                     CKTNDVDIVLMDIRMKVCDGVLGTRLIKEYDSSIALLILTTFNDDEYIEDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMYPQKTIEQKHISDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILGKLELRDRTQLVIFAFKNKIVI"
     gene            complement(11416..>12249)
                     /locus_tag="KPG09_002861"
     CDS             complement(11416..>12249)
                     /locus_tag="KPG09_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0967453.1"
                     /translation="IFSFIIGLDKNLYLALESSSTLLIITLFSVYLENENLSKLYNQS
                     LYDKLRVSKDELKKANADLEIYANSIEELAILKERNRISREIHDSVGHSLSTTIIQLN
                     AIEKLLKDKPLISDLVHELREFVTESFQDVRRAISELKPIEYENYQNLLKIRELVNSF
                     IKLTNINVRLTISKNTWNLSTNQSIALYRLVQESLSNASRHGKATEIRIFITFNPSNL
                     IITISDNGIGCNNIKRGNGLSSISERIYELNGKVEFDNSNTGFTIRASFPKFSGGDFF
                     E"
BASE COUNT         4646 a         1436 c         1956 g         4211 t
ORIGIN      
        1 cgaataatct aaaaagtctt aatattttaa ttgttctgaa tagttaattt tatcaaggaa
       61 aattagagaa aaaacaaaaa accaactatg tttaaagtat acaggttgat tttgtttaat
      121 taagaaagtt gtattaaata ttgtgaagat tttataaatt atgaaacata attgacaaat
      181 ttaagtcaaa aatgtataat taaataaaat ctcgtcaaaa ggttttcatt taatgaagag
      241 attggactta ttttttagta aaaggtagta aaattcaaat gggggtgctt taaatgggtg
      301 agaaacaaat caattttaaa gatgtaattg taattggatt tgcactattt gcaatgtttt
      361 ttggtgcagg gaacttaatc ttccctccat atctaggagt tttatctgga agtagttggc
      421 taatagcttt cataggattc ctttttgcag atggtggttt ggcattatta gcagttatag
      481 cagcaactaa gttcaatgga gacacatcga agatgttctc aagagcaggc aaaggattaa
      541 gtattgcatt aggatgtgca atggtaatct gtatagggcc acttttagca atacctagaa
      601 cagcagcaac aacttatgaa atgggtatat tgcctacaat aggttctgga ataagtccag
      661 taatattctc tatagtattc tttgctctag tattggtttt aacaataaga ccatcaaaag
      721 ttgtagatat agttgggtca atattaactc cagctttact tatagcttta gcagtattaa
      781 tagttaaagg tatcgtatct ccattaggag aaataagaga cagttcttta attcaaaatg
      841 tatttgcaga aggtataact caaggttatc aaacaatgga tgcattggca gctagtgtat
      901 ttgcatcaat tataatcatg tcagtaatag ctaaaggtta tacaggtgag aaagaaaaga
      961 tgaaagccac aataagtgca ggtgtaattg cagtaatagg aatggcatta gtttatggtg
     1021 gattatgtta tcttggagca actgtgtctc aacaatatgg acaagatgtt caacaaacag
     1081 ctttaatagt tgcaataaca gcagctttac ttggaactac aggtaaaatt ttattggcta
     1141 ttatagttgc attagcatgt ttaacaacag ctataggtct ttcttcagca gctggtcaat
     1201 atttctcaac acttacagat ggaaaattaa agtatgagca tatagttata gtagtttgtg
     1261 tatttagtgc aatcatatca aactttggag taagtacaat aataaaattc tcttctccta
     1321 tactaagtat ggtatatcca gcaactataa ctctagttat actagcatta tttagtaata
     1381 aaattaaaaa tgacaacgtg ttcaaatgtg ctgcatacat ggcattatta gtaagtgtat
     1441 taacagttgc aacaagtttt ggtgtaaaaa ttcctctagt taatagctta ccatttgctt
     1501 cattaggatt taactggata gttccagttg taatagcagg aataatagga aactttattc
     1561 catctaaaag cagtaaaact gcataataat aaaactaatt gataattgtg gaattgcctt
     1621 taaattatat ggcaattcct ttttatattt aaaagaatta ttaaattatt gtggagaaaa
     1681 gaacattatt aatctcattt gataaattta tttttggagg agtatgattt ataaagacaa
     1741 taaaataaaa aagatttatt tataattata aataaaatta aattatatat tatgaatgtt
     1801 taatattaat ataaaaacat caatatatat ttttgtaaag aaaaaataaa gaatttattt
     1861 tctgaatact tattaattaa tcaaagttaa aatgaaaaaa atataaaaaa ataaagtata
     1921 taagagtata atttgacttt tatatacttt tttatattat ttttgagttt tttaggaaga
     1981 tattttacaa gttaagaaaa ctataaggag tgttatattg aatataaaat agtataatat
     2041 atattatgtt agataattaa tatgaaaata tacaaatata taaaaaagtt tgtttagagt
     2101 tatagttaat gtgtgtatta atttaatact aatttattgg aggtttaaaa atgaaataca
     2161 taataaaaag agatggtaca aaagaacttt ttagtaaaag taagattgaa gatgctgtct
     2221 ataaagctag tataaatagt gaaggtggag tagatagaga gttggcatat gatatatcaa
     2281 gaaaaatagc taatgactat aattctatgg aaaaatgctt atccgtagaa gaaatacaag
     2341 atatagttga agtactactt atggaatcaa atagaaaaga tatagcaaaa cactacatat
     2401 tatatagaga aagacgctct gaaataagga ctaaagcttg ggagtttgat gaattacaaa
     2461 aatcaatatg gaagaacaag tatagacata atggtgagag ttttgatgaa tggataaaga
     2521 gaatttcaaa taatagttca agaatatcaa aactaataag acaaaaaaga tttctatttg
     2581 ctggaagaat tttagcaaat agaggattac agaaagaagg aaaaaaagta acatattcta
     2641 attgttatgt aatagaacca ccaaaagata gcatagaaga tatatttgat actgcaaaaa
     2701 aacttgctag aacttttagt tatggtggag gagttggaat tgatatttca aagctaagac
     2761 caaaaggagc gaaagttaac aatgctgcaa aacatacgac tggagcagtc tcttttatgg
     2821 aactttacag tatgacaact ggcttaattg gtcaaagagg gagaagagga gctcttatga
     2881 tttctatgga tgtaaatcat ccggagatag aagagtttat agatataaaa acagatttgg
     2941 agaaggttac taaagcaaat atttccataa gggtaacaga tgaatttatg caagctgtaa
     3001 gggaaaaaaa gttatataat tgtacttttg aagttgaaac tactggagaa aaaatagtta
     3061 agatagtaaa tgcttatgat ttatttaata aatttgtgaa aaataactgg gattatgcag
     3121 aaccaggtat attgttttgg gataaaataa aaacatataa ttttatgagt gaagataaga
     3181 acttttgctt ttcaggagta aatccttgtg cagaagaacc acttccaagt ggaggaagtt
     3241 gtctacttgg ctcaataaac ttgtcagagt ttataataca accatttggt gaacatgcta
     3301 cttttgatat taataaatta aaatcttgcg ttagagaagc tgtagtagct ttaaatgatg
     3361 tcttagatga ggggttgaac ttgcatccat tacaagaaca aagagatagt gtacataagt
     3421 atagacagat aggtttaggt gtaatgggta tagctgatat gcttataaaa atgggaataa
     3481 gatatggttc tgatgaatct ttagaactat gtgatttaat tggtaagact atgttaaatg
     3541 aagctgtgaa gcaatcagca ttaattgcta aagaatatgg aacatttagt gagtataaat
     3601 atgaaaatat ttctaaatct aagtttttta tagaaaatct aaatgaagac actaaagaaa
     3661 tggttaaaaa gtatgggctt agaaattcac aacttttaac catacctcct acaggttcta
     3721 tatctacaat gataggtatc agtggtggaa tagaacctat atttaatctt tcatatataa
     3781 gaaaaactga aagcttgcat gatgaggatg tgtattataa agtatatact ccaattgtta
     3841 aagagtatat ggacaagaaa ggtattacag atgaagaatg tttaccagat ttttttgtta
     3901 ctgctatgaa tataggatat gaagaaagaa taaacatgca aaaagtatgg caaaagtata
     3961 ttgatgcttc tatatcatct acaataaata taccttttga aactacagtt gaagatgttt
     4021 tcaatatata tattaaggca tgggaggctg gtcttaaagg agtaacaata tttagagatg
     4081 gatgtaaaag gagtggaatt ttaataaatg aaaaaaagga agaaaataat aaagaagata
     4141 aaaaagaaaa tagtaaaaaa gaatattata aaaataataa aaaagaaaaa gataataaag
     4201 atgaaaaagc caaagaaata ataaataaaa attttgaaga agatgaaaaa tttgtgtgcc
     4261 ctgaatgtgg aagtgaagga attgcacata caggtggatg tagtatatgt ttaaaatgtg
     4321 gctatagtgg atgcaattaa actctaatta aaaaatttta atatatttaa aaattaaatt
     4381 tttaaaaacg aaagatgagg gaatggtatg caggataaaa gtgtgaggga gattaaagaa
     4441 ataatagaga ctttagaagt agaaaaatat atggaatata tagaactgtt aagagttgat
     4501 gaaagaaaat ctgtacaagg tctagctata aaactagcaa aaaagctgga taatattaga
     4561 aaagaagaag aaagattgga aactataaat atatttgaga acgaaggcta tgataaaggt
     4621 tatttgtata ttggaggaat tgatgaggcg ggaagagggc cacttgctgg acctgttgta
     4681 gcttctgttg tagtatttaa aaaagataca aaaatagaag gtgtaaatga ctctaagaaa
     4741 ctgagtgagg caaaaagaga tgaacttttt gaagtaataa aagaagaagc actagattat
     4801 ggaataggta tagtaaataa tgaagaaata gatgaattta atatattaaa tgctacatat
     4861 atggcaatga aaaaggctat aaattgttta aaaaaagcac cggattattt attagttgat
     4921 gcagcaacaa taccaggtat tgacatatct caaaatccaa ttgtgaaagg agattcaaaa
     4981 tctatatcaa tagcagcagc aagtatactt gctaaagtta ctagggatag tataatgtac
     5041 caatatgata gagtataccc agaatatggg tttaaatctc acaaaggata tggaactaaa
     5101 gaacactatg aagctattga aaaatatggc ataactccta tccacagaaa aagttttttg
     5161 aaaaatatac tgtaaatata atatgatata atgtatataa tataaattac tatagttaat
     5221 gggggattaa agatggaata taatgaatta ttaaaaagtg ctagagaaaa tttaaatgga
     5281 agttgtaaag tttgtaaggt ttgtaatggg gttgcttgtg caggtgaagt gcctggcatg
     5341 ggaggtaaag gaagtggttc ttcttttata gaaaatagca agagtttaga aaaagtaaaa
     5401 gtaaatatga gagtaataca tgatgtatca aatcctgata catctataga gatgtttgga
     5461 aagaaaatga gtgcacctat atttgcagct ccagtaacag gaactacttt aaatatgggt
     5521 ggaaaaatta atgaaagaga ttacatagaa ccagtagttg caggatgtgc taatagtggt
     5581 atttatgcaa tggttggtga tacggcagta gatgcttttt taatggaaaa tttagatgta
     5641 gtcaaaaagt ataatggagc tggaattgta tttattaaac cttgggataa tgaaaatatt
     5701 ataaaaaaga taagattagc tgaagaagct ggtgcatttg cagttggagt tgatatagat
     5761 gcctgtggtc tagtaacttt atcattacat ggaaaaccag tacttcctaa aaatgttaaa
     5821 caaataaagg agttagtaaa gtctactaaa ctaccattta tattgaaagg tataatgact
     5881 gttgaagatg ctttaatggc agtagaagca ggtgtgtatg caatagttgt atcaaatcat
     5941 ggaggaagag tgcttgactg tacaccagga gcatgtgaag ttttaccaaa aattgctgat
     6001 gctgttaaag gtaaagttac aatacttgca gatggtggag tgagaactgg tttagacgta
     6061 ctaaaaatga taggtttagg tgcagatgca gtattaatag gaagaccttt tgtgactgct
     6121 tcatttggtg gtgcaactga tggtgtagaa acttatgtaa ataaacttca atcagagcta
     6181 agtagttcta tgatattaac tggatgtcaa actataaaag atattgatgg aaaagttata
     6241 tacaaataaa attttataca aaaaagagga aaaatcattt taatgtagaa taattgtaca
     6301 aagggggaat ttatatgagt agaaaagctt atgaagaagc cttagtagaa ttagaaaaat
     6361 ttattgatga aagaaaagaa ataattaaaa gtgcggagga ctgtattgat aagtacatag
     6421 ttgacagaac gctacctttt gattacaaag ataaatgtgt ggaatggcaa caagagttac
     6481 ttgatattgc agaggcacaa gttttagaag caaacgaatt aggtgtttta ctggaagaga
     6541 aaaaagaact tgaggaagag taagttttat tactaaatct agggaaaatc aagaatatga
     6601 tttaccccta gattttttta tttaaataaa gatgtctata taattaatct atctttatca
     6661 atgttagatt aaaaggtttg aagaaaaata gagtaattgt atgaatagtt aaacaattaa
     6721 actagtctat aagaccaatt taattgttat tttttgaatt tagtatatgg tttttcattt
     6781 cttctaatac aattgtgtag gatttgcatt gtgctgcagc caattctgac aaagcaaacc
     6841 tttgagcctt atttagaaag tccttatctg tgctacatag ttgttttgaa ttatcttgta
     6901 tcataagcat cttaattaat tttacaatct caaaaacatc tcctgaatga aatttatcat
     6961 ggtatagttt aagtcgtttt tttctatcaa aaacccataa acaatcaacc ttatcaaggg
     7021 attggataaa gttttcaact tcattttcat ccataattgg acgtacacca atcttatcag
     7081 catttaaaac tggaatcgaa attttaagat tactattgta aataacagtc aattcatagt
     7141 atttctgtat atgatgattt atatcctttg ttacaagacc acttatataa caggctcctt
     7201 cttttggata cattacactt tcaccgattt tatacatatg gttatacctc caaactgaat
     7261 tttagatatt gcaatttggg tacaatgctt taattatcta ataattaata ttatatcacg
     7321 aaatgcttga caagtcaaaa agattaatat ataataaaaa gtttagaata atagatatat
     7381 ataattggct ataaaaatat aagataagag gatttaatat attaaaatat ctagttagta
     7441 ttgtataaaa taacatacta actagattta ataattatta atttctagca aaatctctta
     7501 agttaagact tccggctgtt acaaatacca ttccaataag aattaaaaca attatattta
     7561 ttaatatatt accattatta ttaatagcat ctatagtcca ataaaatggt gtaagctttg
     7621 aaagattagc cataaaggat aatttatcaa gtgatggtat catattagat atggatatag
     7681 ctaaacctat taaagaataa aacgtagaca taagacttat agaaccttcg tttttaaaca
     7741 ccctagctat ccaaaataca aatccagtag caacaaaact atttgctagt acaatcatca
     7801 atgtatttag agtcaattta aaattactta tagatgtgaa acatactaaa gttaaggtgt
     7861 atacaattgc ttgagtaaaa aataacccta aaaatatgct gaaaattatt tttatatcat
     7921 tatgctttgt agaaacaagt ctttttaagg tattagattt ctttaatgaa aataaatcag
     7981 aagctagatg tgctgcatta atgtacatga aataacatat caagaaaact attaaaaatg
     8041 aatttttatt cttacctgaa ctgttatcaa ttatatttgt cttggtcaat ttatcatcta
     8101 tatttctatc tatttttaat tttaatgaat tagtaataaa agattctatt tgtgattctg
     8161 cccaaagact acctccacca ttttcagttt taaaacattt aacctcaggt cgttttaagt
     8221 tatctatatt gcttgaaaag tttttatcta aaatgaagac agcagaaact ttattatttt
     8281 ttaatagatc catagccttg tcttgttcgt ttaatccaaa tgagttttct tttaatttag
     8341 aactatctat taacttatgt tcgtattttc cagaagaatt ggatacaatc gctactggag
     8401 aaaagtacat tgattgattt tctgagttgc cagtaaaaaa gttaagataa aacattaata
     8461 taacaggaaa caaaaccata ttaactaaaa gagcatgatt ttttatatat ctttttatgt
     8521 ttgcaatagt caacctaatc attattaaac ctccctccat ttatattttt cttttacaat
     8581 agaaagtgca atcaatactg tagatgcaat taggcatgta aaaattaaac tttctattga
     8641 atctatagtt ttaaacgtct cataattact gaacaatgtt cctattaaat aagtaggaga
     8701 taaacttgta aacttagtaa acacatcatt tactatgctg aaaatacctc caaaaactgt
     8761 ttgcaagaag agtaatgcat aaactataac tgtaccatat ttcttgctaa aaaaggtagt
     8821 tataaaagta gatattgaag tcataaaata agaagctaaa gcacaaagta aagctaatat
     8881 aacaaagtta cctttgaatg ttacaccgag tgttctaaaa aataaaacgt ataatgataa
     8941 aaatataaat gagtaaatcc agaaacctat aaaatcataa attaaaaatt gtactctagt
     9001 tacaggtata gagtagagtc tttttgaaag tccttttgat tcacttacat aatttgataa
     9061 aatgctattc attataaaaa ttattgcgaa gaatccaagg gaaactaaag caaagtactc
     9121 ataagagttt tgtttaacat tattttttat tatagaggta tcaatagaat tcttattaaa
     9181 taattttgag aaatcttctg aacttaattt ttgagaatta ttcagatata atttttcatg
     9241 ataagtatct aatatatctt gaagtaatat tgcaatatta tcattagatt taagtttttc
     9301 tatacttaga ttatttgatt tttcatttaa taaattattc tcatatcctt taggtatagt
     9361 aagttttaag tctgccttag aatcatcttt ttgaactgtt aatatagaag ataaatcgtt
     9421 ttttagatat cctgtcaaat tacttgatag ctttgtatta tctttatcat atataacaat
     9481 tgatgtcaaa tctgttttta taggattttg aaatatactt tctgtcacca tcccaagaaa
     9541 tccagctaaa actagaggta aggctaagaa atatattaaa ttaagcataa aggagctttt
     9601 taaaactttt agcatagttt ttacactaat gtacaatttc atgaaatcac ctccattagt
     9661 cccttaaatt tttacctgtt agatttaaaa atacgtcttc taaactaggt tcttcataag
     9721 atattcgtgt tatttttgaa ttatttttgt caacaacaga taatacatct gtaagttgta
     9781 attttttgtc tacacctaat tctaataatg aattattttc taaaatagat acaacacctt
     9841 tagtattttt taaatcaaat ataagttgag catttatatt ttttatttcc ataattaatt
     9901 ttgtatttgt agatactaat gattttaaat aatcattatc tccaaaagca atctctttac
     9961 cttcatccat aataaaaatc ttagaacaaa gatgctcaac ttcttccata taatgagaag
    10021 tatatattac tgtggttttt tgctttttag atatattttt tataaaattg aaaatatggt
    10081 ttcttgattg cgggtcaaca cctacagttg gttcatccaa tattaatatt ttaggatgat
    10141 ttaaaagtgc tattccaatg ttcaatctcc tttgcatacc accagataat tttttgattt
    10201 ttttcttttt aacatcagtg agtcctgtga cttctaaggc ttcagttatt ctctcttgaa
    10261 gcaattttcc ctttagtcca tagtaagtgc caaagtactc taagttatca aaaggagtaa
    10321 gagtctctat aagtgctaat tcttgtggaa ctaatcctat tatagacttt gcttttatac
    10381 aatcttttga caaatctaaa ccattgattt ggatgtcgcc actatttggt aacataagat
    10441 ttgtaattat atttataagt gtagatttac cagcaccatt cggtccaata agtccaaata
    10501 tctctccttc tttaacagaa aagctgatat tatcaagtgc taatttatca ttaaatcttt
    10561 ttgttatatt atttactctt actatatcca taataattcc tcccaaaata attttgtata
    10621 agttaaaaat ctataatact ataataaaga attatgaatc ataattctat tactataagt
    10681 cactaaaaaa agtgacttaa gtcactttat attttcattt attataaatg agtttgtgag
    10741 taagaaattt ctagggtata gatttcttac ctatatattt atattacaat tttatttttg
    10801 aaggcaaata taactagttg tgtcctatca cgaagctcta gtttccctaa gatattggtg
    10861 atattattct ttattgtacc ttcagataag aataattctt ggctgatttc tttgttgtta
    10921 agtccatttg cgattaatct aattatggat atttcttttt ctgttaaatt atacatatca
    10981 gaaatatgtt tttgttctat agttttttga gggtacataa ttttttcagc cacactttta
    11041 tcaagtacaa tatttccaaa aaacgaggaa cgaatccctt catgtaatac ttcatccgaa
    11101 ctatccttta aaagatatcc tgaagcccca aatttcatag catcttctat gtactcatca
    11161 tcattaaagg ttgtcaaaat taataatgct atagagctat catattcttt tataagtctt
    11221 gtgcctagaa ctccatcaca aactttcatc cgaatatcca taagaactat gtctacatca
    11281 tttgttttac aaaattctaa tgcttcatat ccattggaag catctcctac aatttctaaa
    11341 tcgtcataag atgataaaat aatgttaaga ccttttctaa tcaatttttc atcatctact
    11401 ataagaactt tgattttatt caaaaaaatc acctcctgaa aatttaggaa aagacgctct
    11461 aattgtgaat ccagtattag aattatcgaa ttcaacttta ccatttaatt catatattct
    11521 ttcgctaata ctgctaagtc catttcctct ttttatattg ttacacccta taccattatc
    11581 gctgatagta attataagat ttgaaggatt aaatgttata aaaattctga tttcagttgc
    11641 ttttccatgt cttgatgcgt tggaaagaga ttcctgaaca agtctatata aagcgataga
    11701 ttgatttgta gataggttcc aggtattttt tgatatagtt aatctaacgt tgatatttgt
    11761 aagtttaata aagctattaa ccagttctct gattttaagt aaattttggt aattttcata
    11821 ttcaatgggt tttagttcag atatagctct tctcacatct tgaaaacttt cagttacaaa
    11881 ctctctaagt tcatgaacta aatcagaaat gagaggtttg tcttttaaga gtttttctat
    11941 agcatttaat tggattatcg tcgtagaaag agaatgtccc acactatcgt gtatttccct
    12001 cgatatacgg tttctttctt tcaaaattgc taactcttct atagaatttg cataaatctc
    12061 taaatcggca ttagcttttt ttaattcatc tttagaaacc ctgagtttgt cataaagact
    12121 ttgattgtat aatttagata aattctcgtt ttctagatac acagaaaaga gggttataat
    12181 tagtaatgta gaacttgatt ctagtgctaa atataaattt ttatctaaac caataataaa
    12241 agaaaatat
//
LOCUS       DAELSY010000120        11955 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.120, whole genome shotgun sequence.
ACCESSION   DAELSY010000120 DAELSY010000000
VERSION     DAELSY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11955
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.120"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(204..872)
                     /locus_tag="KPG09_002862"
     CDS             complement(204..872)
                     /locus_tag="KPG09_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG0967454.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMTEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     GKAY"
     gene            complement(865..1668)
                     /locus_tag="KPG09_002863"
     CDS             complement(865..1668)
                     /locus_tag="KPG09_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG0967455.1"
                     /translation="MKKLKLIIMLALVLALLITGCSSGNNKNKEEQKTIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMVEPPIQFGAC
                     SFAIQKNEKGEKWTKEINEVIEEMHKDGTLTKISEAWLGKDITKEPINE"
     gene            complement(2075..2497)
                     /locus_tag="KPG09_002864"
     CDS             complement(2075..2497)
                     /locus_tag="KPG09_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0967456.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGACPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            complement(2511..4688)
                     /locus_tag="KPG09_002865"
     CDS             complement(2511..4688)
                     /locus_tag="KPG09_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0967457.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALHSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANVMNIDDDLLKAGYEASIRHMFKKTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDNEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYRESDINSIIDDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            4969..8148
                     /locus_tag="KPG09_002866"
     CDS             4969..8148
                     /locus_tag="KPG09_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0967458.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKRDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNIHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKETTIENKLTSSQFTKENNHYVFKGTDYDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIYKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIIDLFN"
     gene            complement(8329..8412)
                     /locus_tag="KPG09_002867"
                     /pseudo
     CDS             complement(8329..8412)
                     /locus_tag="KPG09_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(8433..9197)
                     /locus_tag="KPG09_002868"
     CDS             complement(8433..9197)
                     /locus_tag="KPG09_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0967459.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(9434..10927)
                     /locus_tag="KPG09_002869"
     CDS             complement(9434..10927)
                     /locus_tag="KPG09_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0967460.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDCNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINKGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKVNELKDIEKSRITHGKILFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            complement(11252..11794)
                     /locus_tag="KPG09_002870"
     CDS             complement(11252..11794)
                     /locus_tag="KPG09_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0967461.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT         4184 a         1861 c         1281 g         4629 t
ORIGIN      
        1 tataaaactt tgtcgaatat taaattacaa tagaatttta tttatttttt caatagaaaa
       61 actatttttt aacttgatta tacagtccat tttgatacta tattttctca ataatttaga
      121 ttactaagtt tatttttcta aaattaaaaa tttatttaca agtaataata tatctacatc
      181 atcccatttt taaatattga aatctaatat gcctttccta ttcttttttc aactttgctt
      241 tgtatattac tcaatattat agatactgcc cagtaaatta atatcgttat catatatatc
      301 tcaaaatatt taaaacttga agcagcctct acttgagcta ctcccattaa atcttgtatt
      361 cctatagtta gacctaaaga agaaccttta attatgtcca gtgtacagct tacaatagga
      421 ggtactgcag atgtagctgc ttgaggtaga attattttaa ccattgactg atatttactt
      481 aacccaaaag cataacatgc ttcctgttgt cctttatcaa cactctcaat agatgctcgt
      541 ataatttcag tcatatatgc agatgaattt acacctaaac aaattactat tgctaaataa
      601 ggtgaacaat ctttcataaa agaaaatatc attggaaatc caaagtaaaa gaaaaatatt
      661 tgaattaaaa gtggtgttcc tctaaagaat gaaacataca ctcttgttat gtattgcaaa
      721 acttttgatt ttgtctgtgt gaaaaaggca agtattacac ctaataatat tgataaaaaa
      781 aatgcaacaa tagatattag tattgttatt cccaatgctt taatcaaaaa tggaaatata
      841 cttagacaaa attcaaaatt aaaattactc attaataggc tccttagtaa tgtcttttcc
      901 tagccaggct tcagatattt tagtcaaagt cccatcttta tgcatctctt caattacttc
      961 gttaatttct tttgtccatt tttcaccttt ttcatttttt tgaatcgcaa aagaacaggc
     1021 tccgaattga attggaggtt ctaccatttt aagatttaat ttttgttctt ttatagctat
     1081 taaagcctca acatcctgcg caactactgc atctaatctt cccatttcta gatcttgata
     1141 attcccttta agagttgcac ttgaataatt tactattttt atttcatcat ttgggtactt
     1201 ttcttttagt atttcatttg ctactgctcc agtacccaca cctactttct tgccaactaa
     1261 atcatctatt gattttatac tagtatcatc ttctctaaca ataatgtttg ttccactata
     1321 attataagca tctgaaaaag catatttttt ctctctttca ggtgttgcag taagttgagc
     1381 agatacaaca tctgctttat cagcttctaa taatccaaat aatccactaa aatcagttaa
     1441 tacccattca actttacaat tagttcgttt agcaaattca tcccaaaaat ctacttcaaa
     1501 tcctttaagt ttatctttat ctttaaaagc aaaagggtaa tatgttcctg atgttgcaac
     1561 tcttatagtt ttttgttctt ctttattttt attatttcct gatgaacatc ctgtaattaa
     1621 aagtgctaat accaaggcaa gcataataat taatttaagt tttttcataa tttattctct
     1681 cctttttaat cttattttaa ttttttatat aatacatttt caaaggttga atcacttgta
     1741 ccattataat tgtttaatgg ctctgccata acataactcc caaatattga tactgtagta
     1801 aaagattgcc tcttatttta taccttacta aatttgcttt tataacacta taaatcaatt
     1861 ttctagttat tatattatac ttcgactttg aacaacataa gtttaatatg ttttacagtc
     1921 tttgtccaat agcaaaaaac tattactaaa aatcacatta tagtatccaa ctatatccat
     1981 aaaaaagcta tattgagttt ttattataac aaactcaata tagctttttg taaaaatatc
     2041 ttataccaag tataagtcac tctctatatt taatttattt attaaatttt aatgctcctg
     2101 taggacatac cattacacaa ggtgaatcca taccacactt atatctctca atacaaccat
     2161 tacacttatt tgccttacca tcatttccat aaattaaggc tccatattca caagcttttt
     2221 cacaaagttt acaccctata cagttttgat tgtctaatac aacgaatcct tcttcttttt
     2281 taaaacaatt tttaggacat gcacctaaac attttggctc attacaatgg atacatgcta
     2341 atgaaaaata ttctatattt tcaccttcct ctattgtaaa tacatgtcta aaaagttcac
     2401 cattgtctat attattatca ttttggtcta tacatgcaag caaacatgct ccacatgctg
     2461 tacataattc tttattaaat ttaaataaac ttttcattat atctctccat ttatctattc
     2521 attttttcaa tcttacactt gcaacttcta tacgcaggga atccactata agggtcaaaa
     2581 ttatcatcaa taatactatt tatatcagat tctctatatc catgatacat atgtatattc
     2641 ttatataaga ctctatcagt aacattgact tttacatcca aacttcctat cttagtagtt
     2701 atttttatat aatctccact gataacaccc aattcttttg catccaagct ggaaatatca
     2761 caactagctt cattttttaa tattcttgaa agtttgatgt catgaagtct tgaatgtagt
     2821 gcatttgaaa ttcttgctcc tgatgtcaat aaatgagtat attcattatc tatattatca
     2881 ttaaatcctc tatatgttgg tagtgcatcc agtccaaaat ctttatattt ttcaataatt
     2941 gtagatttta attcaaacct agaagtgcta gtatcatacc cctctcttgt atatgagcct
     3001 actatatatg gcttaaaatc tttacatttt aatggtaaat cactttcttt aagtttatct
     3061 acatctacac acgtcttttt aaacatatgt cttatacttg cttcataacc tgcttttaat
     3121 aaatcatcat ctatattcat aacatttgct aaatctgaaa gtatttctac atctgatttt
     3181 gaatcgtata atttatcaat aacttttttg gtatatgtaa tataccctcc tgggtaactc
     3241 ttacattctt ctctctcaaa tgatgtacac gcaggtaaaa gtatatctgc atattttgat
     3301 gtatctgtca taaataaatc tatattcaca aaaaaatcta cactatgcaa cgctttaaaa
     3361 agtctccctg aattaggaaa cattctagca tttatcccat gacaaaacaa agctcttatt
     3421 tcatatggtg tcttttcttc tatttgccta ggtaaatcca tagcttgaaa ttgagttacc
     3481 atttcattcc atagaggaaa tctttctcca ccaattcttt catttatatt tttaggtaga
     3541 acttctttta caaactcttt ttctagtgtt tggaatccac ctatcttacc agcaaagtaa
     3601 tcttgttttg gtatgtttcc acctattcta tcataattac ctgttatagc agataaagac
     3661 atcattgctc tatagttttg aaatccattt tgatgatgaa ctattggagc tgcactttca
     3721 tttatacaaa atggaccttg tgacaacatc tcaacagctt ttactatatt atctttagaa
     3781 actcctgtaa ttctacttac agtatctaaa tcaaataatt ttacatattc tttatattca
     3841 ttaaatccat gtacatgctt ttctatgtat tctctatcta ttaaatcctt ttgtataaat
     3901 agattcgcca ttccaagagc taaagctcca tcagttccag gtttaattct taaatgtaaa
     3961 tctgctagtt tttgagttgt cggtgttatt ctagggtcta caattataaa ttttagacct
     4021 ttttctttta attttattag attcacacta tttaaaaatt tagagtagta tggattaaat
     4081 ccccacccta aataagtttt tgcattctcc atatcttgca ttgtaaagtt tcttccagta
     4141 gcaacatttc cagccatctg tgctgatgtt gaacatgaac tagattctgt accataatta
     4201 ggtgaaccaa atgaagatgc aaacctttgc aaaaaaggtc tataccactt tccataaccc
     4261 gtaaaaaaag ctacatgctt tgctccatac tcagatttta ctctatttaa tttttctgct
     4321 atttctctat aagcttcatc ccatgatatt ggtttaaatt ttccttcacc tctttttcca
     4381 attcttttta gaggtgtttt tattctatct tctctatata tatattctct attgcaaaga
     4441 cctttggtac ataacaaccc tttattcatt ggatgttctt ttgtaccttc tatttttatt
     4501 actttaccat ctttaacata tgcatcaatt ccacaatgta ctcctggaga acaaatatca
     4561 cataaagtcc tctttatttc tatacctgtt tccttacagg gtattttagc tttaagttgt
     4621 ctataactat ccatattaac attttccttt cccaataata aatacaaatt attatactta
     4681 ttatacattc tttttattca aatatcatac tttatatttt atatagtgca atttctaaga
     4741 atatttttaa taataagatt ttatatttat taataatcta tttatataat tatttttcat
     4801 tcatagttta taagataatt caataaaaat ctaatagtaa attgttattt acacatatat
     4861 tgtaatagat aaaagctatt catatctgcc ttaattttag tcttaaattt agagtgtaaa
     4921 ttgaatgcat ttagattata atatatacag tgaggtaagg tgaataatat gaaaatagat
     4981 atgatatata aactaataag aagaaataca gaaccatcaa gatggtcaag aggtgtaaaa
     5041 tattatacta atcaattagt tgattatata gacatagata ttgatgacct acaaaataac
     5101 ctgttgacat ttcgtggtgg agtcaattct gaagataatt atagctttta ttttaattca
     5161 ataactatag atataaagca ctttaagata attgaaaatg attgtagttg cgctgactgt
     5221 caatcaaagg gcaaaagtat ttttatatgt aagcatatag ttgctattgt gataaaaagc
     5281 ttaaaacaaa taaaaaataa aagagatgtt cctcctattc taatagatat attaggtgca
     5341 gataaggata agctttctaa tacaaattct aaaacaagct tagatttaag caagcaatta
     5401 ctttctttgt tagacaatac taaaagagaa agggttaatt tagatgtatt gataaatgtg
     5461 gattcaaata aagcaattgc tgaatttaaa ataggaattg ataaaatgta cattttaaaa
     5521 aacattactg aatttgcata ttcaaaactt aatggatatg aattagattt tgggaagaaa
     5581 tttacttaca accccaacat tcattatttt gctgatgaag atgagaagtt agtagatatt
     5641 atagaagaat atgggacaaa tctttcatac aacttttctt catcacattc aaaaaaattt
     5701 atggagataa atgcaagctc tttaaaacaa tttttaaatg ctattaagta caaggatttt
     5761 acccttattt tttctaaaga ggaatatcat ccaaagatat taaatgatac tctacctgta
     5821 aggtttgatt ttaaaaatat agaagataaa atagtcttat attctaaaga ttcactacct
     5881 gttccactta ctaaaaaaaa tgatgtactt ttatatgatg gcaatatcta tttattatca
     5941 cataaaaaag ctcatgatta ttcaaaaata tatcaaatat tatcaaagac taaagagtta
     6001 aaatttgata aagaggattc taaggatgtt ttaggattgc tagttcctag attaaaatct
     6061 atatctcaag aagttcattt agatgataac ataaaaaata atataacaaa agattttaaa
     6121 tctgagttct actttgatat gattgatggc aacatatgtt gtgatgtaaa gtatatatat
     6181 gatgatgagg ataaaaaatt tgtgctcccc aacatacaaa aggaaactac tattgaaaat
     6241 aaacttactt cttcacaatt tacaaaggaa aataatcatt atgtgtttaa aggaactgac
     6301 tatgacctat ttacattttt agatttacaa ttagaatctc taaaggaatt tggagatata
     6361 tattattctg agaagtttaa attgaaaaaa atatataacg cttcatcaat tcaagcttca
     6421 atcaatcaga ctaaccagaa ttacctagaa tttaatttta atatatctga tataaatcca
     6481 aaagagtaca aggatatttt gaaagctttt caagaaaaac gtacttttta taaacttaaa
     6541 gatggaaact ttatagattt atcagaaaga gaaacaaaag atttttttga attagttgaa
     6601 aatctagata taatggataa atcaaaaaat aataaaattt ataaaaataa agctctatat
     6661 ataaatgatg taatacgtag taaaaattta aagtttatag atggtaaaaa agaactagac
     6721 agtatatgtg aaaaatttag aaattttgat agcataaatt tagaaattcc cgcaaattta
     6781 aatgcaaacc ttcgtgatta tcaaataaat ggcctcaatt ggtttaaagt tttagattat
     6841 tataaatttg gtggtatatt agctgatgaa atgggtcttg gtaaaacaat acagactatt
     6901 gcatttttat tatcattatc taataaaaaa agtcttattg ttacaccgac atcactcatt
     6961 tataattgga aaaatgaatt tgaaaagttt gctccagata taaaagtttt attgatacat
     7021 gggaataaaa gagatagaga aaaatgtttt atggaattag aaaattttga tgtaatactt
     7081 acaacatatg gtactcttag aaatgattta gaaaaatata gtgaaataaa gtttgattat
     7141 tgtatattag atgaagctca aaatataaaa aatccagtag ctttagtaac tgagtctgta
     7201 aaaagtataa atgcagaaaa taaatttgca ctcactggta cacctatgga aaataatcta
     7261 ctagaattat ggtctatatt tgattttata atgcctggct atctatatag taaagcaaag
     7321 tttcaggaat tgtttataaa taaagaggat aatgttaaaa acctgaaaaa attaataaaa
     7381 ccttttattc ttagaagaag taaaaaacaa gttatgaaag aacttccaga taagattgaa
     7441 aaaaactttt ttgttgaatt gaataaagag cagaaaaaga tatatagtgt atattcaaaa
     7501 gatatacaag ataaaatgaa agataaaaat ctaaagaaag ataaaatagt tatattttct
     7561 tatttaacta aactaagaca actttgttta gacccaagta tagtagtaaa agattacaat
     7621 aaaaaaagtt caaaaattga aacatgttta gaaatactta gggatagtat aaacgaaaac
     7681 cacaaaatat tattgttttc acaatttact tctgtgctta agaacataag taaggaactt
     7741 gataagtata aaattaaata ccattatata gatggtaaaa ctaatgccaa agaaagactt
     7801 gaacttgtag atgagttcaa taatagtatg gataaaaaag tttttttaat atctttaaaa
     7861 gcaggcggaa ctggtttaaa tcttacatct gctgacatgg taattcactt tgacccatgg
     7921 tggaatccat cagtagaaaa tcaagctagt gatagagctc atagatttgg tcaaaaaaat
     7981 tcagtacaag ttataaaatt gattgctaaa gggactatag aagaaaaaat aataaaatta
     8041 caagaaagta aaaaagagct tataaatcaa tttataaatg gagaactttc taatgaaggt
     8101 gtattaaaaa gtctatctga tgaagagata atagacttat ttaactaaat tatttaatcc
     8161 tacagcttaa ctatataaat ttattctaac atttatatag ttaagctaca atatctgaat
     8221 aaataaaatt tcatattgtt atttaatccc aactttacaa tctaatgata tctctttcta
     8281 tacatattgc atatacaaat gtaataactt gactattaga atattttgtt ttaacaatcc
     8341 tatttcatca taataatgaa gtatatgtac actcactttt gtaagttttg caatccattt
     8401 tacagttttc attattgtaa attttggtta aattattcct ctaaaaattt ctttgctaca
     8461 tttgaaaaat ctaatccatt cgataaaatt gcaggatgac cacctttatc aaacaaatac
     8521 attttaccat ttttaatctt ttcaagaagt aatgaaaaag tattttcaaa aaaatcacta
     8581 gatattagag atacaaactc atcttctcta cttcctgtta aaagcacctc tggttgcata
     8641 gtttctagag gcttatgaaa aaattttcct atagttttat aatgttcaaa aatagcttct
     8701 gtatcattat ctacaacttt ttcccagttt tctccttgat tataaatata aaaagctttt
     8761 gcaccatcat cttgctttga tgcctcacgc tctattttaa cattttgtac aaatgactct
     8821 aaaggaactt ctccttcaaa actatctgca attaccttat tcactaaatc aggtctctca
     8881 agtgcaacat ttaatgcaac aagagctcct ccactactac caattatgtt tactttttta
     8941 tagttcattg cttctaaaaa acatatcact tgcatagcct catcaaacca taagtcagca
     9001 gaaaatttat cgaccatttg agattttcca tgacctacaa aatcaattaa tattacttta
     9061 tattcatctt tataaaaatc gattatatca ttaaacattt tggaagaggc tgtatttcca
     9121 tgtaaaaaaa gtaatggtgt accatcccca atttcattat agtagcactg tttgtcgtta
     9181 tagttaaaat atgccatata ttacctcctt taatatccct ataggtatat atttattact
     9241 tatatttaaa atgactattg tcataaacat ttaaaaatag tatagcttag aatgtagata
     9301 aagtcaacat tcctttcctt aacaacttct attgtttttt gaaacttcat ttcgataaca
     9361 gaataattca taaatattga tactcttact taaatatatg atttcatcat cagagagagc
     9421 agtaataact aaattatcgt atatctcttt tcactttaac catttttttc tttataggta
     9481 tctccttata tttttttaat atcaaatctc ccttataatt taatatatct acatatgaac
     9541 ataaatcttg aacttcaatt acacctatat catcattagt tatacctttt atattactaa
     9601 atgcaccaac tatatctaaa actcttattt tcttcttctt accagcattt aaatataatt
     9661 taacaacttc actttctata tttagtttta tatctttact tttgtaagaa tttttatcaa
     9721 ctttttttct ctttgatact ttttttagga ttccttttga atattcttca aacaaaattt
     9781 tgccatgtgt aattctactt ttttctatat cttttaattc atttacttta tatcctatat
     9841 acgtttctat attctctata tatttttcat ccttttcagt tactatagta attgcctttc
     9901 cactatttcc ttttctccca gttctaccta ttctatgtat atagttttct ttatcttgtg
     9961 gcacatcata atttattact aaagaaatat catcaatatg tattcctctg gacgctacat
    10021 cactactaac taatatatta aatttttggt ttttaaaatc cttaattaca aaaattcttc
    10081 tttcttgtga taaatctgca tgtaactctc ttattaaaaa accctcttta ctcatcttat
    10141 tatataattt agatactttc tctttggtat tacaaaatat tatcacactc tttggatttt
    10201 cgctgtatat taattctttt aaaaggatgt acttctctct tccatttgcc ttgattattt
    10261 tgtcatctat ttgtcttgtt ttttgattag tatcgctctc attttcttct atatttataa
    10321 cactgtaatc tagcatatat ttttcacata tatattttat ctcttcatct attgtagctg
    10381 aaaaaagact tactattttc tccttaggta aatttaataa tattttttcc atttgttcaa
    10441 caaaaccttt attaaacatt ttatctgcct catctattac taaatacttt acattttcca
    10501 acttaataga acctttattt atatggtcaa gtattctgcc tggagtagct acaacaatat
    10561 gaaccctttg tttcaattca gctatttggt cttttataga ttgtttgcca aatatagcac
    10621 tacatctcac cttctttagt ctccctatat ctgatatctc atcttttact tgcaaggcta
    10681 attctcttgt tggaaccact ataagagctt gaatattatt gcaatctaca tttatattct
    10741 cgcataaagg tattgcaaaa cttgctgtct ttccacttcc tgtttttgac ctaactacta
    10801 agttttgacc ttttaataat tttggaataa cttctctttg tactctagaa ggtatattat
    10861 atcctaaact cttcaatgac ttaagtatct tttcatttaa cttaaatttt tcaaatgtat
    10921 atttcatata ttaccacctt attatattta tcttatctat atatttttct acattatatt
    10981 attataatac aaggttctac tatttttata ttatttatta gactgaagat aaaaatatat
    11041 actctataaa cctatagttt agtagcaaat caattaaatt tttatattta aatacctcct
    11101 taaatatact gataaatcaa tatttaggag gtatctcttg tcaataatca aactaatcta
    11161 tattttgaaa gtttaaatta tactaataca ttgtattttt atttatatct aatattaaaa
    11221 ggtattaatc aatacctaaa tcaccataat ctcacatatt tatatcttta ttcaatatac
    11281 cttctgtaat caatcgtgct atataatcat aacctgattc atttgctttg ttacaatcat
    11341 tcacattatc acagaaaaaa ctttcaatct gtattgcaac tggatttgta tttctcaata
    11401 tatataaatt ttctcttttt ttagctcctc tattttcaaa taattctcca agtctattag
    11461 atactctttt agcatattct aatccttcag tgccatagta aagcacttct gtaccacatt
    11521 gtgttccatc agctttattt aaatgtagct ctactaataa atcgtattta ccactgttta
    11581 cttttggaac tctatagaaa aattcttctc tttctgacat aaagtgcctt tctggacaaa
    11641 ttattacatc tactgtatta ccttgactta taagcatctc agctaacata ggagctaatt
    11701 ttttattata attgtattca tcaactatac catttacacc tgtactttta ccacctttta
    11761 gtatactatg acctactgtt atgcatattt tcatttactt ttttcacctt cttttaagcc
    11821 aatttttata taatagtaat ataattttaa agttattact aataataata tattaattga
    11881 agaaggtaaa agttctccgt gattatcatt tttttaacat atttgtctta ttttatcata
    11941 agtctatatt tttta
//
LOCUS       DAELSY010000121        11795 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.121, whole genome shotgun sequence.
ACCESSION   DAELSY010000121 DAELSY010000000
VERSION     DAELSY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11795
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.121"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..592)
                     /locus_tag="KPG09_002871"
     CDS             complement(<1..592)
                     /locus_tag="KPG09_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0967462.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKEKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLD"
     gene            complement(623..2341)
                     /locus_tag="KPG09_002872"
     CDS             complement(623..2341)
                     /locus_tag="KPG09_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0967463.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLNIGSYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMKSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIIDNIRVAIANKKWNDDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            complement(2567..3805)
                     /gene="fabF"
                     /locus_tag="KPG09_002873"
     CDS             complement(2567..3805)
                     /gene="fabF"
                     /locus_tag="KPG09_002873"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBG0967464.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            complement(3834..4058)
                     /gene="acpP"
                     /locus_tag="KPG09_002874"
     CDS             complement(3834..4058)
                     /gene="acpP"
                     /locus_tag="KPG09_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG0967465.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            complement(4112..4861)
                     /gene="fabG"
                     /locus_tag="KPG09_002875"
     CDS             complement(4112..4861)
                     /gene="fabG"
                     /locus_tag="KPG09_002875"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBG0967466.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTRVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            complement(4861..5811)
                     /gene="fabD"
                     /locus_tag="KPG09_002876"
     CDS             complement(4861..5811)
                     /gene="fabD"
                     /locus_tag="KPG09_002876"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBG0967467.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIEILLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            complement(5827..6756)
                     /gene="fabK"
                     /locus_tag="KPG09_002877"
     CDS             complement(5827..6756)
                     /gene="fabK"
                     /locus_tag="KPG09_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBG0967468.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            complement(6865..7851)
                     /locus_tag="KPG09_002878"
     CDS             complement(6865..7851)
                     /locus_tag="KPG09_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBG0967469.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            complement(7877..8899)
                     /gene="plsX"
                     /locus_tag="KPG09_002879"
     CDS             complement(7877..8899)
                     /gene="plsX"
                     /locus_tag="KPG09_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBG0967470.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            complement(8910..9467)
                     /gene="fapR"
                     /locus_tag="KPG09_002880"
     CDS             complement(8910..9467)
                     /gene="fapR"
                     /locus_tag="KPG09_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBG0967471.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            complement(9674..9850)
                     /gene="rpmF"
                     /locus_tag="KPG09_002881"
     CDS             complement(9674..9850)
                     /gene="rpmF"
                     /locus_tag="KPG09_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBG0967472.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            complement(9875..10399)
                     /locus_tag="KPG09_002882"
     CDS             complement(9875..10399)
                     /locus_tag="KPG09_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBG0967473.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            complement(10492..11688)
                     /locus_tag="KPG09_002883"
     CDS             complement(10492..11688)
                     /locus_tag="KPG09_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBG0967474.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
BASE COUNT         3465 a         2352 c         1228 g         4750 t
ORIGIN      
        1 tatctaaact tattaaactc tcagaatatg ccattaaatt tttacgtatt tctttaacat
       61 caattctaag accatttatt atttctataa aagtagatat atcaatatat ctattaccaa
      121 ggtcgattac tttatttata taacttggta ctttttcctt tactccaggc gttatagcta
      181 tgtctatatt tttataaatt tttccttcaa tatatggagt aaactttaaa ttttttactc
      241 ctattttgta aaataagctt atcgtttcta aggtagtttc ttttgtatca tttacaacca
      301 atacttcagt tccatttggt attgaaaata atccatatat ttcattttct ttaagtgttc
      361 tccttatcac tataatctta ctatcttgta ctaaatgctt tctcatactg tttaatctat
      421 catcattcat tactagtaca aaatcacctt gtattttatc attctctttt aaaacttcca
      481 gataatagtg gtttatatca atataattac ctaaaaccaa acgtatattt tcttctaaga
      541 aagatgatag tttagttatt ttatcagtta ctatagctat tgattgtttc atatttatac
      601 ctcttattta tatagtatat aattacttat atactacttt atttctacca agttgttttg
      661 ctttatataa atttttatca gcttcttcta gtacatcttt gctatctgaa atatgagcta
      721 cacctgcact tatagtcaca gtcacatcat cattccactt tttatttgca attgcaaccc
      781 ttatattgtc aattatatct gctacttcct caccagattt atttcttatt aatacagaaa
      841 attcttctcc tccgtaccta aagactttat catcttgttc taacataaca caaatggtat
      901 ttgttattcc ctttataaca gtgtcaccaa atttatgtcc ataattatca tttatgagct
      961 tgaaattatc aatatctatc atcacaagtg caaaattatc atctttctct ctgtactttt
     1021 catacataat atcaaaactt tttctattat aaacatttgt aagaccatca tgtaaatttt
     1081 gtgctttaaa atacttcaat cggttgtata aaaaagtaga ccctaatata gcaattaaaa
     1141 ttactgtaaa taatatttta taatatttta tttctttttt agttttttca aatagcttac
     1201 tttcgtttga tactttatca attatataga aactagtctc tgaatctctt atacttttgt
     1261 tctctttata ttcatttatt aacatctttt gatactcatt taaagattct atatttcctt
     1321 gtttttcata tatatttaat atcttattta aattttttat tttctcctga gttaactttc
     1381 ttttggtaga tatctctaaa ctttttttat aattatctat tgcagaatta taattttcgc
     1441 ttttatattc taagtttccc attgccatat aatagtacat atctttatct aatatataaa
     1501 ctgttctatc tgattttata agataatcag ctttttctaa aaaagtcttt gccttttgta
     1561 tattattttt gcccacatac gcatttgact tcattataga ggccattata tcaaaatcat
     1621 tgtaattatc cgtatttaca aactttttat aatcttttat cctgctactt atttcaatag
     1681 ctttatcata ttctccctct tctacatata tttctgctag atttataata gcatataatc
     1741 taaccattcc atttttttca ccttcatcaa tatctatttt aaaagaatcc tttattaatt
     1801 tctctcctgt ttcatagcta cctatattta aaaatatgct cgccaaatca acatctagtc
     1861 ttgctttttc ataattattc cccaatttct cagccaattt tatagacttt acagttaaat
     1921 ctactgcctt agaataatta ttatccatta tatacactga tgctaatctt ttttctatat
     1981 taaatctaca ttcatcagat aatttcacat ctgattctct catcttttct aaagaatcaa
     2041 tgactatctt cgttccatct ggaacatatg aaatagtctt taacatatcc caaacttcat
     2101 tatatacttt attgtatttt ttttctggaa tagattcgaa agaagtctta accacttttt
     2161 ttgcttcatc atactcctta tctgccacaa ggctttcaga catatatttt cccttattta
     2221 taatttctct ttctttcaat ccattttggt catactttat aaacaaaata atactaataa
     2281 gtattataca tataaaaatt atctttttgg gtaacctttt ctttggtagt ctaaaattca
     2341 aaattttagt cctccttttg tatattgtag aatgaaaact tgcatatata aataacctct
     2401 taataattaa taatacaata tatcaaaatg atttacaaca tatgctagac ttaaatctta
     2461 ataaaaaagt ctcctaaaat aatttaggag actttaaatt taagctaata taaatttata
     2521 cagttaaaac aagtgcttgt tttttagttt tatatttaat atttatttat acatattttt
     2581 ttagtactat tgtagcatta tgcccaccaa atcctaaaga atttgatagt gcatatctaa
     2641 tatttttttc tttaccttga tttggaacat aatctaaatc caaatcttca ccaacttctt
     2701 gtatgtttat agttggaggc acaaaactat cttctatact catagcacaa acaacagcct
     2761 ctatagcacc tgatgctcct aataagtgac cagtcataga ttttgttgat gacacacata
     2821 agtcatatgc cttttctcca aatacagttt ttatagccat agtttcatat aaatcattgt
     2881 aataagttga agttccatga gcatttatat aatctacttc ttctaatgga acatttccat
     2941 ctttcaaagc catattcata gacctagctg ctccctctcc attttcagct ggagttgtca
     3001 tatggtatgc atcagcagtt aaaccatatc caactacttc tgcatatatt ttagcccctc
     3061 ttgctaaagc atgttctaag tcttctaaga taacaatacc tgctccttct cccattacaa
     3121 aaccatctct gtctttatca aacggtcttg atgcagtttt agggtcatca tttctagttg
     3181 acatagcctt catgttacag aatccagcaa aagcaagacc tgttacagca gcttcagagc
     3241 cccctgctac catgatgtct gcatcacctc tttgaattac cttaaaagca tcacctattg
     3301 aatgagttcc tgatgcacaa gctgtacata cattagtgtt aggccctttt gctcctaaaa
     3361 atattgaaac ttgaccagct gccatatttc ctatcatcat aggtacaaaa agtgaactta
     3421 ctcttttatt tcctttttcc aaaagaattt tatgttgagt ttctatagct tcaactccac
     3481 ctattccaga acctattata acaccaaaac gttctctatc aactttatct aaatcaagat
     3541 ttgcatcttc aactgctaat ttagcagata ctattgcaaa ttgagcaaat ttatctaatc
     3601 tcttcaattc ttttttactt atatattctt ctggatgaaa attcttaact tctcctgcta
     3661 tctgagtttg atatgtgcta gcatcaaaat tagttatttt atctatacca gatactccag
     3721 attttatatt actccagaac tcttcttttc ccgttcccaa cggcgtaaca catccaagtc
     3781 ctgttattac tactctttta ttcatcggat acctccactt ttttaaagct cgactattta
     3841 ttagcttcta tatacttaac tatatctcct atagatttga agttttctgc ttctgtatct
     3901 ggtatttcta taccaaattc atcttcaagt gccattatta tttctactgc atctaatgaa
     3961 tctgcttcta aatccttcat taatgatgtc tccatagtta tttctttatc ttctactcct
     4021 aattgctcta ttattatttc tacaacttta ttaaacattg tgtacctcct aaaatttttt
     4081 attttaatag atttctctac tttttaaatt attattgcat taccattcca ccatctacat
     4141 ttattacttg tcctgttata taacttgaca tgtcactaac taaaaatgct acggcattgg
     4201 ctacatcttc tggtttacca taagatttct ttggtatgtt tgctagtgtt gattctttta
     4261 catcatcact tagtactcta gtcatatctg tttctataaa tccaggtgct actgcattta
     4321 tatttatgtt tcttgatgct aactctcttg cagaagcttt tgtaaatcca attacacctg
     4381 cttttgatgc acaataatta gtttgccctg cattacccat aattccaact actgaagtca
     4441 tatttattat tcttccatac ttctttttca tcataggttt agtaactgct tttgtacaat
     4501 taaagacacc ttttaagttt atatctataa ctctatcaaa atcttcttct ttcattctca
     4561 taagcagacc atctttagtt atgcctgcat tattaaccaa tatatcaatt tttccaaatt
     4621 cgcttacaac agaatctatc atttgattca cttcatcaaa tttagacaca tcacatttta
     4681 tagaggtaca ctttaccccc atactttcta tttcattttt agtttttagt gcttcatctt
     4741 ctttagaagt ataattgatt actacatcag ctccaaaaga tgctagtttt tttgctattt
     4801 cttttcctat acccctagag ccaccagtaa caactgctac ttgtcctgta agatttatca
     4861 ttatatctct ccatttctat aattttgtac aaattcatta agagaatcta taccatctat
     4921 attataaata ttgacatttg cttttttatt ggaagctatc ttcttaataa atgcttttaa
     4981 agtttttcca ggtcccattt ctataaaagt atcatatcca tcatttaaaa gtatttctat
     5041 agaatcttcc caaagaacag atgaacttac ttgttttata agcaggtctt taccttcgtt
     5101 tttatagtaa tctgcattta catttgccac cacatcaact ttaggtttag ttatgttaac
     5161 tttttctaat tcactggcca attttacacc agcttctttt aacattgatg agtgaaaagg
     5221 tccacttaca tttaatttaa ctgccctttt tgctcctgct tctttgcatt ttaccattgc
     5281 ttcttctatc ttttcatttt caccagatat aactatttgt ccaggagaat tgtaatttgc
     5341 tacttctaca atacctcctt gtatatcttt aaccacattt tcaagtacat ttctatctaa
     5401 acctaaaatt gctgccatag ctccaacacc tgctggaact gtttcctgca taaacttacc
     5461 tcttttttta actaaacata cagcatcttt aaagtcaatt gcatctgctg caaccaacgc
     5521 actatactct cctaaggata atcctaagca tgcattatat tttaaatcta tttgagcttg
     5581 taccgctttt aatactgcta cactatgtgt aaccatagca ggttgagtgt tttcagtttt
     5641 agaaagttct tcttcatttc cattaaacat aaggtttttt aaatccatat ttaaggcatc
     5701 actagcctca tctattacac ttttagcaac agcattattt tcatataaat ccttggccat
     5761 acctacatat tgagctcctt gaccagggaa tactagtgca acttttccca tcttgctcct
     5821 cctgatttac agttctattg ctggcataat ctctcttgct tgatttacca tagcctctat
     5881 aatttcctta caaggtgtta tttcttttac catagcagca ctttgacctg ccataaatga
     5941 acctttttct atgtctccat ctactgttgc aaatcttaaa gctcctgtac cttttttatc
     6001 caattcttca ggagtagctc cattttgttc catctttaaa aattcttttg atagtttatt
     6061 ttttaatgtt cttactggat gaccagtact tcttcctgtt acaattgcat ctctatcttt
     6121 tgcttttagt actaagtttt tatagtttga atgcacagaa cactcttcac tgcaaataaa
     6181 tctagttcct acttgaactg cactggcacc taatgcaaat gatgctgcaa ttcctctacc
     6241 atctacaatt cctccagcag ctattacagg tatgtttaca gcatcagcaa cttgtggaac
     6301 taagaccata gtagtaagtt ctcctatatg tcctccacct tcagtacctt ctgctattac
     6361 tgctgtagct cctaactttt ccattctttg tgccaaagct attgtaggta ctacaggaat
     6421 aacctttgtt ccagcttcct ttaatctatc catatatttt gcaggatttc cagcaccagt
     6481 agtaataact tgaacttttt cttctataat caaatcaatt atatcatcaa caaatggcga
     6541 cataagcatt acatttactc caaaaggttt atctgttaat tttttacact caacaatttc
     6601 tttctttata gcttcttttg gtgcgtttcc tgctgctatt atgccaagtc ctcctgcatt
     6661 agatacagca cttgctaatg atgcagtagc tacccatgcc attccccctt ggataactgg
     6721 gtattttata tttaatattt tgcaaatttt attcatacaa ttctccttca cttgaaatca
     6781 taaaagtaaa ctttttatta ttaatcaact ttattttgtg tataagttta attttgttaa
     6841 ttaaatcatt ttagatattt tatactataa agtccacttg acaactgaag ccccccaagt
     6901 tagacctcca ccaaagccaa ctaaaactac attatcaccc tttttaattt taccttctct
     6961 ataagcttca tctaatgcta ctggtataga tgcagcagac atatttccat atttgtctat
     7021 atttacataa acttttttca tatcaagctc taatctctta gaagaagctt gtatgattct
     7081 tatattagct tgatgtggca ctaagtagtc tatatcagtt gtatttaggt ttgctttttc
     7141 aagagctctt aaagaagttt ctgccatctt tctaactgca aatttaaaca catcactacc
     7201 tgccatttct attgtatgta gattagcttc tacagtctct ttacttgcct taagtcttga
     7261 acctccagca ggtactttta aacaatcttt tcctttacca tcagagccca tatgtgtaga
     7321 cagtattcca ccctcattta caggaccaat aacagctgct ccagctccat ctccaaacaa
     7381 tactgctgtt gttctatcat cataatttaa aaacttagaa caagtttctg ctcctattac
     7441 aagcacattt ttatagcaac ctgtttccac aaattgcttt gcaatagtta ctccatatac
     7501 aaatccagaa caagctgctg atatatcaaa tgcagtagca ttcactgctc ctattgcatc
     7561 ttgtactata catgcagttg aaggcaaaga catatcagga gtaattgtag ctaatataat
     7621 cagctctata tcctctacgt ttaactttgc acattctata gcttttaagg cagctttaga
     7681 tgctaagtca gaagttgctt cagactcttt ggcaaatctt ctttccttaa taccagttct
     7741 agtacttatc cactcatctg atgtatccat tattttttcg aagtgaaaat tatcatatga
     7801 ttgctcaggt agataacttc ctactcctaa tattccagcc tttgtattca ttatatatta
     7861 aacctccaaa acgagtttat tcatcttcgt tatttttatt ttcttcattg tatttgctta
     7921 taaattggtt aatatcttca acgacttttc cctttgaaaa ttttattcct tgatttatag
     7981 cattttttat agctttagca tcagaactac catgagcttt tataactcct ccatttagac
     8041 ctaaaagtgg agctccccca tactcagaat aatctataaa actttttaat tttcttaaat
     8101 catcctttat aagtaatgct cctattttac tttttgtact ggccataaaa gtttccttta
     8161 taagactcat aactgaaagt gctacacctt cagcagattt aagtaatata ttacctgtaa
     8221 atccatcaca tatgattata tctgtgtatg catttataac ttctctagct tctacattcc
     8281 ctataaaatt taaatctaat tttttaattt cttcatatga ccttttgact aaatcattgc
     8341 ctttgccttc ttctagtcca acatttgcta atgctacctt aggattttca agacctaaaa
     8401 cttttcttga ataaatatta ctcatagctg caaattcaac tagattctta ggtttacaat
     8461 ctgcatttgc gccacagtct gctataagtg tcattcctcg ctttacattt ggtattgctg
     8521 aacataagca aggtctatcg attcctttta ttctaccaac aacaaataat ccaccagcaa
     8581 gtaaggctcc tgtatttcca gctgatatta tagcatctgc ctttccttct tttacaagat
     8641 ttaatgctac taccatagat gaatcttttt tacttcttat agcctttact ggtttatctt
     8701 cgttttctat aatctcagta gtatgaacta tctctaattt atttctatca aattcataat
     8761 ttgaaaattc cttttcaagt aaatctttat ctcctgtgat tattaaatca acctgatatt
     8821 cttttatagc atttactgcg ccttctacat tagatttagg tgcattatct ccacccattc
     8881 catcaattac tattttcata atttccctcc taatctaatt cttcaaagat aaactttcct
     8941 ctaaatattt gctcttgggc tttattgttg ataacaactt ttacataatg ctttttatct
     9001 atatttctat acacttctgc ttttgctata agtctatctt gttgttctat caacttaaaa
     9061 ctttttacat ttgcaactct cataataacc ttaggtgcat ctattatagc cattgccaat
     9121 gaatctgcta gagaaaaaat atacgtatct tttaaagtat tagtcttaga gtaaatcatg
     9181 tcctgtgtaa cttctaacat agatatacct aatcgcccta cagataaatc aatgatttct
     9241 cctgtaattt cctttactcc taaagtcttt acttttgaac tctgagtttc tgctattgat
     9301 ttgactcttt ctcttagctc tggtatgttt agactcattc tatctaatct tatggtctgt
     9361 atacttacat caaacagact tgataattct tcatcagtat aaaaaggatc tgtctttagc
     9421 atatctataa gctctttttg tctttgggct ttacttttct ttttcatgtt ccctccaaat
     9481 tttatagctg taattagtac cagctactaa tagtagtata tagtaacaca caaaaatttt
     9541 caatacttat attattattt ttctattttt taatatgcat aaaatccact tattttctct
     9601 agtaaatcat cttatttttt tatatgtaca aaaaaaacat gtagagtata ctctacatgc
     9661 tttttagaag ctattactta gacacaactt ctttaccttt ataatatcca cagtctggac
     9721 acactctatg tggtaatttt ggctcatgac attgtgggca acttacaaat cctgttgctt
     9781 ccatctttga gttagctgct cttctcattt ttgtgtttga tttagacgtt ttacgctttg
     9841 gtactgccat ttacgccacc tccttagtca aaatttaatt aaataaatct tttaatttag
     9901 caaaacgagg atctatgtat tcctcatcgt ttgtaatttc actgcaagaa cactcttctt
     9961 catttaagtt tgccccgcat acttgacaaa gacctttaca gttttcacta caaagaactt
    10021 tatgaggtac attaaaatct aatgtctgtt ctattatatc aataaggtcg atttcttcac
    10081 catcgaatat aaaaggatca aattcctcaa actcatcttc atcataatct tcttctttta
    10141 ctaaaaaacc ttgtatagaa tactctagtg gtatttctac ttctttaaga catcttgaac
    10201 aattgtcaac aattgtaaaa ttgacatcta tatctagata caaaccttta ttagtctttg
    10261 acactttccc ttttaaattt acaggtgata ctaatttgta aatctcatca caataactaa
    10321 tagtatcaat tttttcacaa aaattcaagt ctattttgtc agtttctttt ctatttattt
    10381 tttctatgct aactttcatt aagttcacct caacatatca ccaaatttat tatacaaatc
    10441 tagcatttgt ttgtcaagta tttttacttg ataggcatag gtttaattta attattttac
    10501 caatcttaat gtatctctag ctatcattaa ttcttcatta gttggtatta aaagcatttt
    10561 tactttagaa tcagcagtag atattactgt ttcttttcct ctaacatcat tagcttcttt
    10621 atctaatttc attcctaaaa actccatgtt agaagcaata tctgctctta tagcctttcc
    10681 attttcacca acgcctgcag taaatactac aacatccaat ccattcattt cagcagcata
    10741 agcgcctata tatttttgta cttttttaac atacgcttct aatgtagcct gagctctttc
    10801 gtctccattt ttagctgcat cttctatatc tctgaagtca ctagaaattc cagtcattcc
    10861 atatactcca gattctttat tcattaattt atccaatcca tcagcatcta aaccttcttt
    10921 tctcattaag aatggtaata tagctgggtc tatatctcca catctagttc ccattattaa
    10981 tccttctaat ggagtgaatc ccatagatgt atctacacat tttccaccat caacagcagc
    11041 tatagaagca ccatttccta agtgacaagt tactattttt aaatctttta tatcttttcc
    11101 taatatctct gctgctctct gagatacata attatgagaa gttccgtgga atccatatct
    11161 tcttacccca tatttagtat atagctcatg aggtaaacca tataagtatg aagatttagg
    11221 cattgtttga tggaaagcag tatcaaatac acctaccatt ggtacatctg gaagtatagc
    11281 tttacaagca tctattccca ttatgtttgc tggattatga agtggagcta aatctataca
    11341 ttcttttaaa gcttcttcta cttctggagt tattactact gaagttgcaa atttttctcc
    11401 agcatgtaca actctatgtc ctactgcatc tatttctttc atttctttaa ctgcaccaaa
    11461 ttctggatga gcaacagctt ctaaaactaa tgctattgca tctttgtggt ccttcattgg
    11521 ttgttcaaca acgtatttat catctctacc tgctttttca tgttttaata tagaaccttc
    11581 tattcctatt ctttcaacca atcctataca taaaacttct tcattattca tatctattaa
    11641 ttgatatttt aatgaagaac tcccacagtt taatactaat attttcatta ttatttcctc
    11701 cttagatttt tttataaata atatgtaata aattttgatt tgtaataaat gtctaaataa
    11761 aactctatct tttattattt tgttatagat atatt
//
LOCUS       DAELSY010000122        11778 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.122, whole genome shotgun sequence.
ACCESSION   DAELSY010000122 DAELSY010000000
VERSION     DAELSY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.122"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..325
                     /locus_tag="KPG09_002884"
     CDS             <1..325
                     /locus_tag="KPG09_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0967475.1"
                     /translation="KYVEKNYKQPIKLEDVSYEVGFSVYYFTKFFKKNTGKTFITFLN
                     EYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVSPLEFKKRYNSLEKK
                     GGASK"
     gene            730..2178
                     /locus_tag="KPG09_002885"
     CDS             730..2178
                     /locus_tag="KPG09_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG0967476.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIIHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            2204..4594
                     /locus_tag="KPG09_002886"
     CDS             2204..4594
                     /locus_tag="KPG09_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG0967477.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHTRILDLNEGIVKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIELKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKDWI
                     NAAQNIYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLNYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKKDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGKNISILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            4596..5267
                     /gene="pgmB"
                     /locus_tag="KPG09_002887"
     CDS             4596..5267
                     /gene="pgmB"
                     /locus_tag="KPG09_002887"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG0967478.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLERGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            5306..6706
                     /locus_tag="KPG09_002888"
     CDS             5306..6706
                     /locus_tag="KPG09_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0967479.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNENKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIEKFINKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVKESTDNVVEEV"
     gene            7023..7865
                     /locus_tag="KPG09_002889"
     CDS             7023..7865
                     /locus_tag="KPG09_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0967480.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            8010..9938
                     /locus_tag="KPG09_002890"
     CDS             8010..9938
                     /locus_tag="KPG09_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0967481.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKFVPDLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMTIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVASPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            9999..11426
                     /gene="ascB"
                     /locus_tag="KPG09_002891"
     CDS             9999..11426
                     /gene="ascB"
                     /locus_tag="KPG09_002891"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG0967482.1"
                     /translation="MSFKDDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYRGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLVGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     INYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
BASE COUNT         4678 a         1202 c         2143 g         3755 t
ORIGIN      
        1 caaatatgta gaaaaaaatt ataaacaacc tataaaatta gaagatgtgt cttatgaagt
       61 aggatttagt gtatattatt ttactaaatt tttcaaaaaa aatacaggaa aaacatttat
      121 aactttttta aatgaatata gattagaaaa agcaaagtgg atattactta ataacaatat
      181 atccattgta gatttggttg aagaagttgg ttttggaagt acaaaaactt tttacagagt
      241 atttaaagaa aaaattggag tctctccatt agaatttaaa aaaagatata atagcttgga
      301 gaaaaaaggg ggtgcctcaa aatgaactta gttcatgagg cactttcttt tatatgaaat
      361 caaatatatt ttcagtattt caacaatgaa aattccattc atttataaag tctaaatttt
      421 agttctcaat acaatctcta tttcgaaata aatcctttta tatatataaa tatacagcaa
      481 tatatgagaa taagataaca ataatgagga tataaaaatg tatttgctat atatataatt
      541 atatacatag atatctaata ttgatattta atatgaaagt attgaaaata gacaatatta
      601 attatagtga gtaaaaaata taattggatt tttaaattga attatttaaa aggtatagta
      661 tgaaaaaaat cttataacta aatatgagat agaaattata aaactttata ctgtgaaaga
      721 ggtagatgca tgaaattagg gaaagatttt ttatggggag gggcaatttc agccaatcaa
      781 agtgaaggat tttatttaga tggaggaaaa ggtttatctc agtttgataa actcccatta
      841 aatgataaga gattaaaaga tgtatgttta gatgaggata atattttaaa tgaaaataat
      901 caatactatc caagtcatat agggattaaa ttttgtgaaa attacaaaga agatatagca
      961 atgttagcag aaataggttt taaatgtttt agattttcta tatcttggtc aagaatattc
     1021 ccaacaggag aagaaagtga accaaatgaa aagggattac aactttatga taatataata
     1081 aaagaattaa aaaaatataa tatagagcca ttaataacga taagccactt tgatatacct
     1141 tattctctag ttgaaaaata ttctgggtgg gctgatagga gagtggttaa tttatatgta
     1201 aaatatgctg aaattttgat gaaaagatat aaagatgatg tcaaatactg gattcctttt
     1261 aacgaaatga atatgataat tcatattcca tatataggtg ctgggttaac ttttatagct
     1321 gatgaaaatc aacttcagaa aaaatatcaa gcagcacacc atcaattatt agctaatgca
     1381 aaaactatag aaataggaag gtatataaat aaagaattta agtttgggtg tatgttagct
     1441 gctggaaaaa catatccata cacatgtaaa ccagaagatg tatttgcaac tttagagcat
     1501 gaaaggcata atttgttttt ttctgatgtt caagttaggg gtgtgtatcc aagctttatg
     1561 aattattatt taaaatcaaa tcaaataaaa ttaataataa aagaggaaga tttatcaaca
     1621 cttaaggata atactgtaga ttttgtatct tttagctatt attctagtgc ttgtagttca
     1681 gctagagctg aaggtttaga aaaatcaaag gcaaacggac cagaaaccat aaaaaatcca
     1741 tatcttccaa aatcaaatag tgtatggcaa aacgaccctg tagggttaag aataactatg
     1801 aatcagttat atgaaaggta tcagttacct ttatttatag tagaaaatgg actgggtact
     1861 caagatattg taaatgaaga tggaaaaata attgatgatt atcgtataga ttacatgaaa
     1921 gaacatatta aaaatatgat tcttggagta aatgaagatg gaatagagct tataggatat
     1981 cttatgtggg gatgtataga tgtaaccagt gtaagtgaag gaaagatgac aaaacgatat
     2041 ggcataattc atgttgattc tgatgactat ggaagaggct catttaaaag aactaaaaaa
     2101 caaagtgctt tttggtataa aaaagtaata gaaacagatg gagaaatttt atttaatgat
     2161 atggaaactc atgcataatt cattttaaga aaggagtgtt attatgagtt taatatttac
     2221 tactaaggac caacttatag agcaagggtt tagtaaggat acaataaaat ccaatgaaac
     2281 gttgtttaca ctagctaatg gacatttagg agttagaggg aatattgaag agtccgattt
     2341 taataatgag tatgtagata atatgggtac atatgtaaat ggattttatg aagaatctcc
     2401 aattacatat ggagaaagcg cttatggcta tgcaaaacaa aatcagacta tctgtaagtt
     2461 acctaatgct catatagtaa atttctctat tgaaggagat tgctttgatt tatcaaaagg
     2521 tattacaagt gaacatacta ggatactaga tttaaatgaa ggaattgtta aacgttcatt
     2581 tatatgggaa aactctcgtg gtagaaaagt taaagtatct atagaaagat tagtatcaca
     2641 agatatagaa gaattaatga tgatttcata tacaataaca ccattgaatt ttaatggaaa
     2701 aataaaattt aaaaataaaa tggaaaatag tatggagaag tttaattgtg aaaaagtaga
     2761 tgacccaaga gtggcatcta taaataagaa gcaatttaaa attaatgtaa aaaaatataa
     2821 caataagtat tttatgaatt tagttacaga aagaagtaga ttagggttat ggtgtgggtt
     2881 tgattcatat attacttgtg gaaatattac aaatgagacc tataatgttt cagatgaatg
     2941 tattgagcga gaaatagaag tctatgccaa aaaagatgaa aggttaaatt tagaaattgt
     3001 tgtaggctat agtagagtat attctgattt aaatctaaat gaagaacgtt taaaatcatt
     3061 agaatgtctg gagtcgttaa aatgtttaga atcagaagca catttaaaat cattagaatt
     3121 ggttttaaga gaagcatgta atattggatt tgataaagtc aaaaatattc agagaaagaa
     3181 aatgaatgag ttttggacaa cctctgatgt agagattgaa ggcgataaaa ctcttcaact
     3241 gggtataaaa accaatttat ttcatattta tcaatcagca ggaagagatg gaaagactaa
     3301 tatatcagca aaaggtctta caggtgatgg ctatgagggg cattatttct gggatacaga
     3361 aatgtatata ttaccatttt ttatatatac tcaaccatcc attgctagag ctttattaga
     3421 ttatagatac tcaatcttag atgcatcaag agaaagagct agggaacttg gaataaataa
     3481 aggttgctta tattcatgga gaactataaa tggtaatgaa tgcagtgcat attatccagc
     3541 aggaacagca cagtttcata taaatgcaga tatagcttat ggtattagaa cgtattttga
     3601 agcaactaat gatgaagaat ttatgatgca aaatggctta gaaattctta tagaaacagc
     3661 aagattttgg gttgaatttg gagattatat agaattaaaa aataataaat tctgtataaa
     3721 tggagtaaca ggaccagatg aatatacagc aatagtaaac aataattact acactaattt
     3781 aatggctaaa tataatttaa tatatgcagt acagatggta gataaattta agcataattt
     3841 attttcaaat gaagtattta gaaaagttaa attacaagaa tatgaagtaa aagactggat
     3901 aaatgccgct caaaatattt atttaccata tgatgaagag aagaaaataa gcaaacaaga
     3961 cgattcattt ttagaaaaat cagtatggga ttttaaaaat acaccaaaag aaaaatatcc
     4021 attacttctc aattatcatc cattaacaat atatagatat caagtaaata aacaagcaga
     4081 tactgtactt tcagcgttgc ttttccctga tgagtttagc ttagaacaaa agaagagaga
     4141 ttttgaatac tatgaaaaaa taactactca tgattcatct ttgtcaaggt caatatttgg
     4201 aatgatggca agtgaaattg gaaatcatga aaaagcttat aactatttta tggacacagc
     4261 attaatggat attaataacc tacaaaaaaa tacaagtgat ggaatacatg ctgctaatat
     4321 gggtggaaca tggatgagtt tagtatatgg atttgcagga atgaaaataa aaaaggataa
     4381 attaagtttt gaaccaagga tacctaaaca ctggaaaaat attaaattta atatattatt
     4441 tagagataat ttagtttctg taaatataga agaatataaa tcttattata agttaatcaa
     4501 aggtaaaaac ataagcatat tacatgataa taaagaaata aaattatcaa agaacataat
     4561 agaagttaat aattgtttta aagggagtaa ttaatatgaa agcagtctta tttgatcttg
     4621 atggagttat aacagatact gctaagtatc attttgaagc ttggcatgag atagcaaaat
     4681 cgctagatat agaaatagat gaaactttta atgaaaaatt aaaaggcata agcagggaag
     4741 aatcattaga taaaatattg ttatctgcta ataaagtaga tagttatact aaagaggaaa
     4801 agttaaaggt gtgtgaagaa aaaaataaca tttatataaa gttaataaac tcattaacat
     4861 ataaagatat attaccaggg ataaatgagc ttataaaaga acttaaaaaa aataatgtga
     4921 aattgggaat agcatctgct agtaagaatg cacctaggat attagaatct ttgggaataa
     4981 aagatgaatt tgattgtata gtaaatccat ccttattaga aaggggaaaa ccatatcctg
     5041 atatttttat tgaagggagc aagatgctaa atttaaaaca atatgattgt gtaggaatag
     5101 aagattctta ttcaggtatt aaagctataa atgctgccaa tatgacatca attggtgtgg
     5161 ggaaaaaaga agtactagga aaatgtgacc atattgtaga agatactagc ttattaaatt
     5221 acaatacact attacaagct tggagtaaaa agattaattt ggattaaaat tatattaaaa
     5281 attattttaa aagagaggat aaggtatgaa gaataaatat gaagaagtag ctcaattgat
     5341 aattgatgga cttggaggaa aggataatat aattgactta actcattgta tgacacgatt
     5401 aaggttcatc ttaaaagatg aaaagaaagt caatgcagat aaattaaaat ctatagataa
     5461 ggtagttgga gtaaactcaa cttcaactca atatcaggtc ataataggta atgaggtagg
     5521 tgttgtattt aatgcaataa taaataaggg gataaatacc acaggagata ttaaaagcga
     5581 tataaaaaat gaaaataaaa aaaatagtat tttaaataat ataatagata caataactgg
     5641 ttgtatgaca cctatgatac cagcccttac agcatctggg atgataaaag ttatattatc
     5701 tctttgtacc acttttaatt ggctatcatc aaattcaagt acatatagag tattagatat
     5761 aataggagat gtagcatttt actttatgcc tataatactt gcaataaatg cagctaaaaa
     5821 atttaatgtt aatacatcaa tagcagttat agtagtaggt gtgtttttac atccaaattt
     5881 ctctgcatgg gtatcaagtg gggaccctat atcatttata ggagtaccaa ttcaaggtgt
     5941 tatatatgca gcatctgtta tccctgcact tttaacagta tggatgatgt catatataga
     6001 aaaattcatt aataaattaa cacctagcat gttaaaaaca attttaaatc caactttagt
     6061 attattaata agtgcgccaa tagcattaat agtaattgga ccaataggaa atttcttagg
     6121 agagggatta gctagtataa taaatttact acaaggaagg ttaggattta taatggtttg
     6181 tttattagca gctgcaatgc catttatcgt tatgacaggg atgcatcatg cattgactcc
     6241 tatattcata tcagcatttg caactacagg acaagaatcg ctaatattaa tatctcaagt
     6301 ttgtgctaat ttagcgcaag gtggcgcgac attagctgta gcattaagaa gcaaagatag
     6361 taagatgaaa cagttagcat cagctgcatg gatatcagct atcatgggta taactgaacc
     6421 agctctttat ggagttacat tgaaacttaa aaaacctgct atgttagcat ctataggagc
     6481 aggaatcgca gggttatttg caggaattac tcatgtgact ttatatgttc cacaaaatag
     6541 tataatggca gtacttggat tatcagggga aaagggaatt tcaaatatag taaatggact
     6601 tatagttata tttatagcat tggttgctcc atttattatg acatatgttt ttggagttga
     6661 aaaaaatgtt aaggaaagta cagataatgt agtagaggaa gtataatatt aactatttac
     6721 attaatagaa tttttattat tattgactat aacttgtttt gtatttatat tgatgcaaag
     6781 aaataagtta atattaatga taataattat tataactgac aaaaagcaac acaaaagatt
     6841 gatgaagtat atggaaataa aaagttgaca aaatatgtct gatgttttat catattaatt
     6901 ggaattaggt agtaattgtt taagtacaag ctaagcttat tcattaataa tagcagtatc
     6961 ctaggaagga gtatgcccat tattatggat aagctttttt tatgcttaaa aggaggataa
     7021 tcatgataat taataagata ttaaacaata atgttgtaat cacattagat gataatgatg
     7081 aagaagttat cgttatgggt aaaggaattg gttatcaaaa atcaaaaggc aatctaatag
     7141 ataaaactaa agtaaataaa gtatttagaa tatcaaataa agaaatatca aacaaactac
     7201 aagaattact caataatatt cctatagaac acatgaaatt atcaagtgaa attatagaat
     7261 acgcacaaat aaagttaaac aagaagctaa atgaaagtat ttacatatcc ttaagtgacc
     7321 atacatattc tgcaattcaa agaatgaaag aaggaataaa tgtaaaaaat gcaattcttt
     7381 gggaaattaa aagattttat aaagaagagt ttgaaattgg aatgaaagca ctagatatta
     7441 ttgaaaacaa aacaggtata aaattaccag aagatgaggc tggatttata gcctttcata
     7501 tagtgaatgc tcagttaagt gaaggtcata ctttggcatc tgatattacg aagttaatac
     7561 aagaagtact tagcatagtt aggtatcatt ttaggataga atttcatgaa gaatcagtgt
     7621 tttattatag atttataatg catttaaagt tttttgcgca aagattgctt ttagatagtg
     7681 cacatgaagg tgagacagac aaagaattac taagcattat taaatctaaa tataataaag
     7741 agtttgaatg tgtagttaaa attaaaaatt tcattaaaaa acaatataac tacatattaa
     7801 cagatgatga gcttatatat ttaacaatac atttagctaa agtagttaaa gactcaaata
     7861 tgtaagtgaa ataatttttc agtagtatat taagaatgat tgaaaggagt gatactaaca
     7921 aaattatacc tatggttgga tggtaacatt gagttggcca aaccttcaaa tttcaaaaaa
     7981 ttaatttaaa cgaaatatgg aggagaaaaa tgggaaaata caaacaatta gcacaagaga
     8041 tagtaaacaa tgttggtgga aaagaaaata ttagtggatt agtacactgt attacaagat
     8101 tacgttttac tttaaaagat gaaagtatag caaatgacaa tgtattaaaa aacatggaag
     8161 gtattgttac tgtaatgaaa agtggaggac aatatcaagt agttatagga aatcatgttg
     8221 aagctgtcta taaagatgta gttgaaataa ttggtttaga tgattcaagt acaagttcag
     8281 aaactaaaaa atcaggtaat atactagata aaggtataga tataatatca ggaatttttc
     8341 agccagtgtt aggaataatg gcagcttgtg gtatgctgaa aggatttaat gcattatttg
     8401 tagcaatggg attatatagt gatgtatctg gtggttatat ggtaataaat gcagcaggag
     8461 atgcattatt tacattcctt cctttgttct taggatatac ttcagctaag aagtttggat
     8521 taaaaccaat gttgggatta gcactaggag ctgcagtatg ttatcctgct atacaaggta
     8581 gcagtatatc agcaggagca gatgtgatgt acactttatt taaaggaaca atgtttgcat
     8641 caccagtata tattgatttc tttggactac caattgtatc aattgattat actggaacag
     8701 tcgtaccaat tatatttata gtgtattttg cttctaggtg tgaaaaatta tttaataagt
     8761 ttgttccaga tttagttaag tttttctttg tgccaatgct tacattatta ataacagtgc
     8821 ctgttgcact tatagttata ggtcctatcg caacatttgg ttctacactt atttcacaaa
     8881 ttgtaatttc aataagagat tttagtccac tattggcagg tgctatagta gggtttactt
     8941 ggcaaattct tgttattttt ggtatgcact ggggatttat tcctgtatat ataaacaatg
     9001 ttatgacttt aggatatgat aatgttatga tgccattctt tgcttgtaca tttgcaagtt
     9061 cggcagttgt gttggcaata tttttcaaga ctaaagataa aaaactaaaa gagatgacaa
     9121 taccaaactt tatatctggt atatttggag ttacagagcc agcaatttat ggaatattat
     9181 tgcctttaaa gaaaccattt ataataagtt gtattgctag tgggattgga ggcgctttct
     9241 acggattctt taatcttaga aaatttataa ctggaggtat gggaatattt gaacttccag
     9301 caatgattga accagatggg ggaatgggca acttaatagt agctctttct ggtatagcta
     9361 tatcaatggt cgtagctttt gtattaacta tgattttata taaagatgag aaagtagaag
     9421 agtctaaaaa agtaagaaat aaggggaaag aagaaataaa agaagtcaag tctactaaat
     9481 tagaaagaga aatagtagca agtcctataa aaggtgaagt attaaaatta agtgatatag
     9541 aggatgcagc atttgcatct ggtgtactag gtcaaggggt ggcaataata cctagtgatg
     9601 gaaaagtagt tgctccagta gatggtgtag ttacaacctt atttcctagt ctacatgcta
     9661 tagggattct ttctgataca ggtgttgaag tgcttattca tattggatta aatactattc
     9721 aattagaagg gagaggtttt gaggcatgta taaaacaagg tgatagaatt acaaaaggtc
     9781 aaactatatt aaactttgat gttgatgcaa taaaagagtt ggggtatagt actgtaactc
     9841 caattgttat tacaaattca tcacaatttt tagatgtagt agaaacagaa agtaaaaata
     9901 ttgaattaga agataatcta attacagtct tattttagta aaaattgagg cattagcata
     9961 agctagtgct ttggttataa tttaaggagg ctttgactat gagttttaag gatgattttt
    10021 tatggggagg agctactgca gccaatcaat gtgaaggtgg atacaatgaa gacgaaagag
    10081 gattggcaaa tgtagatgta tgtccaacag gaaaagatag aacagcagtt attacaggga
    10141 aactaaaaat gtttgatttt gatgatgagc attattatcc agctaaaact ggtattgata
    10201 tgtatcataa ttataaagaa gatataaagt tatttgctga aatgggattt aaagtatata
    10261 gaatgagcat tgcatggagt cgaatatttc caaaaggtga tgaagaaaca ccaaatgaaa
    10321 agggacttca attttatgaa aatatattta aggagtgtaa aaaatataaa atagagcctt
    10381 tagtcactat tacacacttt gattgtccaa tgcatttagt taaaaagtat ggtgcatggc
    10441 gtaatcgaaa aatgataggt ttctatgaga aattatgcaa tgttatcttt agaagatata
    10501 gaggattagt gaagtactgg ctaaccttta atgaaataaa tatgatttta catgctccat
    10561 ttatgggtgc tggtatttgc tttgaagaag gtgaagatgt tgaaaagata aaatatcaag
    10621 ctgctcatca tgagcttgta gctagtgcaa ttgcaactaa aatagcacat gaagttgatt
    10681 caaataactt agttggatgt atgtttgcag ctggaagtgt atatccttat agttgtaatc
    10741 ctaatgatgt atgggaagct acaaagttag atagagaaaa ttacttcttt gtggatgtac
    10801 aatctaaagg taaatatcct aactatgcgt taaagtatat ggagcaaaaa ggtattacac
    10861 cagaaatgga acctggtgat attgaattat tgaataaata tacagtagat tttatttcat
    10921 tttcatatta caatagccgt tgtgttagaa ctgatgaaaa tgcagatgat atggctgaag
    10981 gaaacatatt tacatctgct aaaaatccat atctttcata cagtcaatgg ggatggccaa
    11041 ttgacccatt aggtttgaga attacactaa atcatgtata tgatagatat gaaaagccac
    11101 tatttattgt agaaaatgga ttaggtgcta aagatatagc tgatgaaaat ggttatgtag
    11161 aagatgatta cagaattaat tatctaagag aacatataaa agctatgcat gatgcagttt
    11221 ctatagatgg ggtcgattta ttaggttata ctacatgggc acctattgat ttagtaagtg
    11281 ctggtacagg tgaaatggaa aagcgttatg gatttatata tgtagataga gataatagtg
    11341 gtaatggaag tctaagaaga atgaaaaaga aaagttttga atggtataag aaagtaattg
    11401 cttcaaatgg tgaagattta tattaaacaa gaaatctatg ttaaataaga aatttgtatt
    11461 aaataagaag agtataaaaa ttaataataa gtataatttt taactttttt aactatagaa
    11521 aatcaataca gaattatttt acattgtatt tatgcaatag tatgattcac aaataaaatt
    11581 ttagaaaata aaattgttaa gaagtatggg aagtaagtgc aatttacttt gtaataaaag
    11641 atgaaaaagg cgagcttagg ttttaagctt gtctttttta ttatgtataa actatgatac
    11701 tttaaaattc tatgaaaaat ttatttaata gagaaattaa aaatttaatg gtaatcaaat
    11761 tctttaccaa tgaatata
//
LOCUS       DAELSY010000123        11743 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.123, whole genome shotgun sequence.
ACCESSION   DAELSY010000123 DAELSY010000000
VERSION     DAELSY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.123"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(109..795)
                     /locus_tag="KPG09_002892"
     CDS             complement(109..795)
                     /locus_tag="KPG09_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0967483.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINLFKQKVKAFLNHLTTKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            complement(953..2017)
                     /locus_tag="KPG09_002893"
     CDS             complement(953..2017)
                     /locus_tag="KPG09_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HBG0967484.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            2279..2950
                     /locus_tag="KPG09_002894"
     CDS             2279..2950
                     /locus_tag="KPG09_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0967485.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKACLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            2952..3860
                     /locus_tag="KPG09_002895"
     CDS             2952..3860
                     /locus_tag="KPG09_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0967486.1"
                     /translation="MFILAFIICTVVIFIVSTKINQRRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKSEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            3937..4623
                     /locus_tag="KPG09_002896"
     CDS             3937..4623
                     /locus_tag="KPG09_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967487.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            4620..7175
                     /locus_tag="KPG09_002897"
     CDS             4620..7175
                     /locus_tag="KPG09_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0967488.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYIVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETAMEGFVNDYLQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKRGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTGEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKS"
     gene            complement(7369..8568)
                     /locus_tag="KPG09_002898"
     CDS             complement(7369..8568)
                     /locus_tag="KPG09_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBG0967489.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            8687..9574
                     /locus_tag="KPG09_002899"
     CDS             8687..9574
                     /locus_tag="KPG09_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0967490.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            9764..10321
                     /locus_tag="KPG09_002900"
     CDS             9764..10321
                     /locus_tag="KPG09_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0967491.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(10561..11421)
                     /locus_tag="KPG09_002901"
     CDS             complement(10561..11421)
                     /locus_tag="KPG09_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0967492.1"
                     /translation="MKKQKSPILLEMLGLFLGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
BASE COUNT         4474 a         1505 c         1639 g         4125 t
ORIGIN      
        1 attagtttta aatcatcagt tattagataa attgaaatat taaataaaat agctgtaaaa
       61 attataccaa cagcaattat taaataaata gaactaaaag aaaatttttt aaaattcatt
      121 tctttcacct aaccacatat atcccatacc atataccgtt ttgatatact taagattgtc
      181 tttttcaatt ttgcttctaa tcctacttat aacagatgtt aaagtatgtt catctacaaa
      241 gttagcatct atatcccaga gtttttctaa aagtactttc cttgttaaaa tacttttagg
      301 attttcagta agcagtttta aagtacgata ttctaatggt gtaaatataa tttgatttcc
      361 atttatattg gcagataact cagaaaagtt aatttcaaga tatccatccc tatagtaatc
      421 ttgttttgtt tttgttgtaa gatgatttaa aaaagcttta actttctgtt taaataaatt
      481 gatattaaat ggttttgtta tataatccaa agctccaagt tcatagcctt taatcatatc
      541 acattccata tcattagcag ttaaaaaaat tattggtata ttgtaatttt cctttatttc
      601 attacataat tcaaacccac ttttatctgg taaattaata tccaaaatta ttaaatcaaa
      661 ttcacattca aaaatagact ttagtgcaga tttagctgta aattttgaag taattatata
      721 tccatcttca attaggttat atgatagagt tttatttaat atagaatcat cttctactac
      781 taatattttt tccatacagt tactcctttt agtttaatat tttataatgt caattataga
      841 ctaaaaacat tgctgaattg ttataaacga gaaaaagtat ttaaaaattt atatatttaa
      901 taaagaacct gtcctatgat aaattctagt acagattctt tattaaataa aattatttca
      961 accaagatgg atttccaggt ataaatattc ttttaccaaa ttttgaagta tactcatcta
     1021 aagctttttg aataagctca ttattaccct taaatgaagt agtattcttt atagtttcga
     1081 gattttggct aaatgggcaa gcagatatac acataccaca atctgtacct aaatatctcc
     1141 atttaatata gcatgtctca tgttcaatta cccaattata ttttccatca tcaccaattt
     1201 taatatcatt agaaagcgaa tgagaagggc agttaaaaga gcatttttta cagaccttac
     1261 aaaaatcttc tagtccaaaa tcaacatatt catcttctaa aagaggaata tctgtagtaa
     1321 caacacctaa tctaagtcta gagccataat ctttatttgt aagtatagca tttcttccta
     1381 tatctcctaa acctgcatca cgagctataa aaacaggcat tactaaataa ttagcatcca
     1441 tatgatttct agcatcatat cctaaatttc ttatatagta acttaaaatc atgccgacga
     1501 tagatgcatc cacatagcac tttgatgttt ctataacttc acatatcatt ggagctctat
     1561 ttatcatttc tttatccatc tcacatccaa atacaattgc atacttatga gttagattaa
     1621 tttcttcacc gtaattttct tcatgtctac ccctatttgt ataaatatga tagtctttta
     1681 atttagttat accaactaca gaagctccat actgctttgc aagaccctta attttttttg
     1741 tcattgactt tgcatctaca tctactttag tatcagaaac ttttccttcg cataaagatt
     1801 tgatatcaga taaaaaatcg aatgcactac ttgccattgg agagtttaac tcattgtaag
     1861 tcattgttcc ttcactacat aaatttggtc tatttcttat actatcatca atttcttttt
     1921 tgtcaggatt tttcttataa taatcattat aagcagtact atcttttttg taatttgctc
     1981 ttgcaaacat agtatttctc tcatcaaccc ttttcaattc tattccccct agtaaaaata
     2041 tatttaaaaa atataacttt attgttgtat aatttaatta tataagaata acgttatatt
     2101 ttcaatttaa gcgtaattaa attgggaaat atataactat atgcttgatt atgatgtttt
     2161 tattttgaaa acaaatttaa atacaatctt tttagtgaaa taaataaaat taaaatattt
     2221 ttagtatgat aatgcaagaa aataattatt tatctaaaaa atctaaagga gagtaaaatt
     2281 gaacttatta attattgaag atgatataaa cttaaatgaa ggtctttttt atgcatttga
     2341 gaatgatgga tttaatgtat ttaaggctta tacaaaacaa gaaggattaa atatatttaa
     2401 tagtaaaaat atagatttta taatcttaga ctgcaatctt ccagatggag atggatttga
     2461 tgtatgtcaa ataataaggg aaaagtcaga tataccaatt ataatgctta ctgcaagaga
     2521 ctcagaaatt gatgaagtaa aaggattaga aatagggctt gatgattata ttacaaaacc
     2581 attttcttta tctgtattaa aggcaagagt caaagtagca ataagaaaaa aatcgaataa
     2641 taaagtaata tattccaatg gaataaaatt ggaccaaaag ttactaaaag tatataaaaa
     2701 taaagcatgt ttggaactta gctctgtaga atataagctt ctaagttatc taattgaaaa
     2761 taaaggtcag atattattaa aggaacaaat tttacaccat atttgggaca gcgaagaaaa
     2821 ttatgttgat gataatattg tatctgtaaa tataagaaga cttagagtaa aagtagagga
     2881 tgacccatct aatccaaaat atataaaaac agcttatgga atgggttatt tatggaatga
     2941 ggtagaataa tatgtttata ttagccttta taatatgtac agttgttata tttatagtat
     3001 ctactaaaat aaatcaaaga agatatgatg aattaaattt gatgttaaat caaatattgg
     3061 aaggtaaaga agttacttat ccagatacga aggatacaag agcgtcaaaa atttctcacc
     3121 aagtaaaaaa aattaaggat atgatagaaa tagaggtaga acagtcaaag tcagaaaaag
     3181 aagcgattaa aggtttaata tcaaatatgt cacatcaatt aaaaactcca ctttcaaata
     3241 taaccattta ttgtgaactt ctggagaata taaatatttc cacattacag aagaaggagt
     3301 ttttacaaaa gatgaaaaat gaaactttta agattgactg gttgttacaa tccttattta
     3361 aaatgactaa attagaggat ggagttatag aatttgaagt agaagaacta ctaattaaag
     3421 atactcttat tcaaagcatc agtactatct ttaataaatc agaagctaaa aatataagga
     3481 taaatctcga accattttca gatataaagt tagttcataa taaaaagtgg accatagaag
     3541 caattgtaaa tgttttagaa aatgcaataa aatactcacc atcagatagc acgattacta
     3601 tttctgtaat aaaaatggag ctatatacga agattactat taaagatgaa ggaataggta
     3661 ttgatagtag agaactaaat gatattttta aaagatttta cagaagtaaa aatgtggaaa
     3721 accaaaatgg cacagggatt ggtctatatc ttacaagatt aattttagaa aaagaaaatg
     3781 gaaacattat agtagaatct aaactaggaa gtggatgttg ttttagtata ttcttacaga
     3841 attgtaagag tttaaactaa ctttgtagga tgagtgtaat tatcgtcact tacaatattt
     3901 tatagaacat atatctgtat tgaaaggaga aattaaatga gtatattagt gacgaataat
     3961 ttagtgaaac actatggaaa aggtgagact aaagtaaaag ccttaaatgg agtaagtata
     4021 gaaattgaac gagatacatt tactgctata gttggaactt ctggttctgg aaaaagcaca
     4081 ttgttaaata ttataggtgg attagataat ccaacctcag gtgatgtaat aattaaagga
     4141 aagaacatat ctaaaatagg aaaaaaagat ttgacagtat ttagaagaag aaatatagga
     4201 tttatatttc aaaactacag tttaatgccc gtattaaaca cttatgataa tatagctctt
     4261 cctgttacat tagataaagg aaaccatata gaccatgaat atattgaaat gctgatgaag
     4321 acattgggaa tttgggataa gcgtttaaaa tttccaagcg aactatctgg tggtcaacaa
     4381 cagagggtag caattgctag agctttggca aataaacctg cacttatatt agcagatgaa
     4441 ccaacaggaa atctcgatag taaaactaca atggaagtgg tatgtttatt aaaagaaagt
     4501 agtgcaaaat tccatcaaac gatacttatg gtaacacaca atgaaaatat agcacaagta
     4561 tgcgactcaa ttatccatat tgaagatgga gttgtggtga atactggtgg tgaagtacta
     4621 tgatgtctct agtaaaattt tcaattcaat atataaagca ttataaaaaa caatctatat
     4681 ctatagtttt aagcatagta ctatcagtag cattattgac gggaattggt tctttagttc
     4741 atagtgcaga caaaagtaga attgaaaaga taagggaaga tagtggagat tatcattatt
     4801 tctataaagt agataaggaa caacttaata aaattaaaaa aaataaaaaa tcaaaggaat
     4861 acattgtaga tagacttggc gttacaaaac ctaaaaatca gatagatgaa ccttttatta
     4921 tagaattttt aaatgctgat tcttcatatt tagacatgac aggtagaaaa ttattaaaag
     4981 gaactcttcc tagtaaagat ggagagatag tacttgacac atattctcta aacaatttaa
     5041 atgtaaacaa agaaattgga acagaaatca atttagatgg taaaatctat aagttatgtg
     5101 gtattttatc aagtctttca aacccagaaa ctgctatgga gggatttgta aacgattatt
     5161 tgcagcctaa agaaaaggat tcttatatag tttatgtaaa atatgatgaa aataaaaata
     5221 ttaaaaagca aaattataaa tttatgaaaa aattcaacat atcaaaagaa aaaacttata
     5281 taaactggcc tctttcagtt gaatttggag taaaaccacc aaagaatata gaaaaatata
     5341 atatatttga gttcttaaaa aatttagaac taaatacaaa tgcaattata ttgcttgttg
     5401 gagtgtttgg tgcagcagca gtatatagta ttttccatgt ttcaatactt cagagaatat
     5461 cacaatatgg ggttttagaa gtattaggcg ctaataataa acaactttta tttctactat
     5521 ttttagagtt atttttactt tttattgttg gatttcctat aggttgcttc ttaggtattg
     5581 gagtagcgtc tacaatatat gaacaatttc cacatatatt tttgagtagt gatattgttc
     5641 caggagcatt ttttataagt gaaaaatcta tttatttagg ctttttattt atcttattgt
     5701 tgttaatttt aattgctgta cgtgttgtac atcagttgaa taaatactct agtatagaat
     5761 ctatgaaaaa ctttaagatt actaataaga aaaagagaat attagaaagt gtaaaacact
     5821 ctaacatgac aaaagtatta tctcataaat acatgactca aaaaagggga atgtttattg
     5881 gaattttgtt ttcattatca cttggtggaa ttatatttct atgttctagt tactctatac
     5941 agcttactaa aaacaataat gaacttacaa tgaaagctga tgatggttta aattctgact
     6001 accaaataac tatgcaaaca acagattttg atgtaggtat accaaaagat aaaatttcaa
     6061 gattaaacaa tgttgaggga atatcaaaag tctatccagt aagatattta tttggagcta
     6121 tgcaaattaa agaagaacaa ctattttgga aaaatttctt taaacctctt gaaaaagatt
     6181 acaggataaa gacttttttt gatggtattt gtacaaagca aaatgaaggt ggctatcttt
     6241 taaaaacaaa tatttatggt tataacaatg gtatgttaga aggattaaat tcttatatta
     6301 tagatgggga gattaatacc catgatatgg ttaaaaataa taaggttata gttagactac
     6361 ctatggatgg aactgggatg tatgatgcag ttgatataaa tccaggagat acaataaaag
     6421 ttaaagtgcc aaagacaatg aaaccaacag gtgaaattgt taaatttaaa gaagaaaata
     6481 agaatgatta tacaacaaaa gaatttgtag ttgctgctac tgtaaaacgt gtgatggcaa
     6541 ataacatata tttcattggt gattatggta tggatatagt tatgacgaat gggcaaatga
     6601 aaaataactt tgatatcgaa aattataatt cagtaagtat aaaaaagaca gaagatagta
     6661 acagtgagga attgtctgag aacataaaaa gtgttgtaag tgatgtaaaa agatgtatcg
     6721 taacagatta taccattgct attgaaaaaa ataataccta cttaaatcaa aaactcttat
     6781 ttatatatgg tattgtattt gtacttcttg caataagttt gttccatata atcaatacag
     6841 taagctactt aattttttca agaagacatg agtttggaat tttaagagct atgggtatta
     6901 cggataataa gtttttactt atgatgataa gagaaggttt tttatatgga ttctatgcaa
     6961 gtattataat ggtaattgga agtgttattg gacaatttat gatttatttt atggtaaaac
     7021 gtgtatattt atatataaat ccaattctta aaataaatac acctctatac ataggtatga
     7081 ttattttaaa tatcactatt agtatagttg ctgtaattat tccagtaaga caaattttga
     7141 agagtgatat tatttcagaa ataaataaga gttagagtta tataaatttt taagattata
     7201 taactttatg acaatcttgt ttaaattatt taaatatatc ttatctgtga acattaaata
     7261 aacctcttaa tagatataac tcttgaaaag tcatatctat taagaggttt taataaattt
     7321 tcatgttaat atatataatc caaaaaaatt aaatcttatt aaaacatact attttgtata
     7381 gttatcgaaa tatccttgta tgtaaataat aggagtaccc ttatctccac ttccagatgt
     7441 taaatctgct aaagaaccta taaggtctgt taatcttctt ggagtagtac cttgagaaac
     7501 catgtttcct gttaaatcat cagatttatt atctttttca actatgtatt ttgaaattgc
     7561 ttcttttaac tcatcaccag ataagtcagc aaaatcatta tctgctaagt attttaattt
     7621 tacttcgttt ggagttccct ctagcccttt tgtataagca ggagatacta ctgggtcagc
     7681 taattcccat attttaccta cagggtcttt aaaagctcca tctccataaa ccattacttc
     7741 tacatctttc cctgttattt cttttatatt accttggatt ttgtttacaa cttcatcgca
     7801 atttataggg aataatttta ctgtttcttc tgtagctttg tttgaaccta aaagtccata
     7861 ctggtcattg taaccactac catctacact tgctgtcatt atatcatcaa gtgaatatac
     7921 ttttttagct ccattttgtc ttaaaattct ctttgttctt tgtcttgtat gtatatcaca
     7981 agttaaaata cttgttgtat agttaagaat agtttttgga ttgtttgcaa atactatttc
     8041 tacctctgca ccacaatcct tgattaaatt cttataataa tcaacataat ctacaccagt
     8101 aaatgtatgc ttgttatatc caaatagttc tctgtatttt tcttctgtta aaacatctga
     8161 ccaaggattt attcccttat catcaagttc atcaatactt attaagtgat ttccaacttc
     8221 atcagaaggg tagcttaaca ttaatacgac ttttctacat ccttttgcaa ttccttttaa
     8281 acatatagca aaacgatttc tacttaaaat tgggaatata accccaacag tatcatctcc
     8341 aaatttatct tttacatctt ttgcaatatt gtcaacgtga gcataatttc cctgtgctct
     8401 agctacaaca gcttctgtaa cggcaattac atctttgtcc ctaacttcaa agttttcact
     8461 ttttgacgca tttaaaacag aatcaacaac tattttaact aagtcatctc cttgtcttat
     8521 aataggtgct ctaacccctc ttgaaacagt tccaactaat ctatccatta tctaaaatcc
     8581 tcctaaagaa ttaatattgt catattagta ccatgtatat tataattcaa atataattat
     8641 aagtaaaata aataatacta atatttgaca taaggagtaa ttatatatga atataaaact
     8701 tgaactttat aaggttttta atgcagttgt aaataataag agtttttctc ttgctgctaa
     8761 ggaattgttt atgtcacagc cagctgtaag ccagtcaata aagcaattag aagaacaatt
     8821 agatacttta ttattttata gaaacaataa gggagtaaaa ttaactccag aggggaaaat
     8881 tctaagtgaa catgtcacta ctgcattaaa gttgatatca tcaggagaag atagaataaa
     8941 taaatttaaa aaattagaat acggttctct gaaaatagga gtaggagata cagctgcacg
     9001 ttttttcttg ttaaaatatt tagaaatttt tcataagaag tatccacata tacatgtatc
     9061 tacaataaat agaacgagta gagaacttat atctttactc aaagatggga atatagatat
     9121 agcaattata aacatgccaa tagaagatga tacactaaat attgttgagt gtattgaaat
     9181 acatgatata tttgtatgtg caaatgatta catagaatac aaaggtagga aaatatcatt
     9241 ggaagaatta aacacattgc ctttaataat gctagaaaat aaagcaaatt caagacttta
     9301 tgtaaatgaa tattttttat caaaaggtat aaaactaaac ccggatatag aattgggttc
     9361 tcatgaatta cttttagaat ttgcgtatat aaatcttgga gtgtcttgtg ttatagaaga
     9421 atttagcata gattatttgg aaaatgagaa attgtttaaa ttagatataa aagagccaat
     9481 acctaagaga aatataggtt actgccattt aaaagatata tctttatcct tagcaaccaa
     9541 agaattttta agcatgatat ctaataacat ataatttaga atacatattt cacaagtaat
     9601 ttaatataaa aatagagata aattcgtaaa tttgaaagat tcatatagta attagttttt
     9661 attaaaaata aagataaggt taatttgaga acatttatat aattaatatc gtattttaaa
     9721 tatgaacagt atatttagat taatgtataa agggggatta gaaatgtctg attctgaata
     9781 tcctcaaatg aaaaccttag aaactagaag ttgtatacta agacctgcat cattagatga
     9841 tgttgaagat ttttttgact gttataaaaa taaaatagta gtaaaacatt taccatttag
     9901 tgagcataaa tctttagaag atacaagaaa gtttataaaa tcattttttc taaatcccta
     9961 taaaaaaggg aaaattggcc actttgctat agtatataaa agagacaata aagttattgg
    10021 gaatatgggg tttaataata taaatcctaa agcattagaa gcagaaatag gtatatgtat
    10081 aaatccaagc tattggggac atgattttgc tactgagata acaaaaagag taatacagta
    10141 tggatttaga gaactaaact taaataaaat aattgcaata acctatgaag aaaaccaaaa
    10201 ttctacaaaa tcattagatt tattaggatt taaatgtaca ggaaagtata tcaaaaaaat
    10261 tcatactgga aatactgtaa aaaatgttcc ttgttatcaa tatgaattga aaaaacaata
    10321 gatataatca tttaatatct atcctggtta tttagtaatg attgttttct aaataaaatt
    10381 tcaataaagt agttgactca aaatatagat aatcctgttt tttattgtta aaatactgaa
    10441 aatgcatttg attttataca aaaaagagtg tctcatgaac taaaaagttt atgagacact
    10501 ctttttgaat aaatcgtatt ttaattaaaa tagtatatat acttaaaata ttaattttta
    10561 ttagttctct aaatttttaa atccttctcc aagaacctca tgcacatctg taacaataac
    10621 aaatgcagta gggtcagttc ttttaactaa tctctttaga taaagttctt gttttttaga
    10681 aactacgact aatagaactt ctttattttc tctagtgtat ccacctttac catcaagaat
    10741 agtaagacct ctatcaagtt cttcagttat agcttgtctt aaactttcag gctcatcaga
    10801 gataataaaa aatgcttttg aatggtcaaa tccttccatt atcatatctg ctatttttat
    10861 taatacacaa agtgctatac cagagtaaag agcagtttct aaactacgat ttactatacc
    10921 agaagatata acaaccattc catctaggca tgacatgaat tttgtagctt ttattcctgg
    10981 gaatttctta ctaagtatga gtgcaattaa gtctgttcct ccagtagaag catctgattt
    11041 aaacattatt cctaaaccta taccaactag gattcctcct gcaatagaag acaataataa
    11101 gtcattagta aatcttagtt ttgataatgg gtctgttaat tgaactatag ctgaaaatac
    11161 aactgttcca aataaagttt ttaatgaatt ttttgtaccc ataattctaa aagcaagtat
    11221 tactaatggg acacctataa ttaacatgat agctgatact ggaagtcctg taaccttgtt
    11281 tagtactaat gatagaccac taagtcctcc aggagcaatt gtatgtggtt ttaaaaacat
    11341 atttatacca atactcattg atatacaacc caaaaatagt ccaagcattt caagtaaaat
    11401 tggcgacttt tgttttttca tataaacacc tccatgaact tgtcacaatt taatattata
    11461 acatatttga ccagtttata ttatataaaa tttcttgata taagcaattt caagtatttc
    11521 attattctta atatttttga atttttatat agtgttttca tatctttact gatgttgtta
    11581 cactcctatg aagtttttta tatattataa tttttttatt cttttataaa gtatccccta
    11641 taaagatggt tctatgaaaa ttttctctat actgtttaac gtatatagag attttgtgta
    11701 taattataag gtatttaatg gaaaaaatta tcttgctttt ata
//
LOCUS       DAELSY010000124        11722 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.124, whole genome shotgun sequence.
ACCESSION   DAELSY010000124 DAELSY010000000
VERSION     DAELSY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11722
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.124"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            7..714
                     /locus_tag="KPG09_002902"
     CDS             7..714
                     /locus_tag="KPG09_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0967493.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            708..2018
                     /locus_tag="KPG09_002903"
     CDS             708..2018
                     /locus_tag="KPG09_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0967494.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMAQE
                     NYLKDTTESEETGYLVEYNEYFKEGTFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEKLEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            2135..2818
                     /locus_tag="KPG09_002904"
     CDS             2135..2818
                     /locus_tag="KPG09_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967495.1"
                     /translation="MKILYTENLSKHYGKGENLVRALDNVNLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            2811..5198
                     /locus_tag="KPG09_002905"
     CDS             2811..5198
                     /locus_tag="KPG09_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0967496.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFELIKGKLPTKENEIVLDSGALKALGYKNKDLGEKIKISYNDYKNDK
                     KIEKEFVISGILKTSEISEAGKYYYAIISELYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGGFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKNNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNLIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKAVISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            5477..6619
                     /locus_tag="KPG09_002906"
     CDS             5477..6619
                     /locus_tag="KPG09_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG0967497.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIVGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLDRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALKLTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNENLLNLFNKYYF"
     gene            6638..7162
                     /locus_tag="KPG09_002907"
     CDS             6638..7162
                     /locus_tag="KPG09_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0967498.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYITEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            7262..8080
                     /locus_tag="KPG09_002908"
     CDS             7262..8080
                     /locus_tag="KPG09_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0967499.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSELFAKKLEESLNPTQSVEKIVLYLEEMLLSILEEDLISNKSV
                     KLLNSFIYDNRYSNIKAFCDNTNINIKTLERICLKYTGYTPKILKRIYRFKMASSQLI
                     YNYKDNELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYNYL"
     gene            8158..8469
                     /locus_tag="KPG09_002909"
     CDS             8158..8469
                     /locus_tag="KPG09_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBG0967500.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(8772..10775)
                     /locus_tag="KPG09_002910"
     CDS             complement(8772..10775)
                     /locus_tag="KPG09_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBG0967501.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     NFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(10928..11566)
                     /gene="recX"
                     /locus_tag="KPG09_002911"
     CDS             complement(10928..11566)
                     /gene="recX"
                     /locus_tag="KPG09_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBG0967502.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
BASE COUNT         4539 a         1288 c         1876 g         4019 t
ORIGIN      
        1 atagggatga ataatatatt acttttggaa gatgacaaat ctttaaatag aggaattagt
       61 tttaaattaa aaaaagaagg atacaatgtt tttagtgcat ttggtataga agaagcaaaa
      121 aatattttcg caaaagaaga gatatgctta attatttccg atataggtct acctgatggt
      181 agtggttttg acttttgtga ggagattaga aagaaaagtg atgtttacat aataatgttg
      241 acagctcttg atgaagaggt ggatattgtc acaggatatg atttaggtgc agatgattat
      301 atcacaaagc cttttagctt gatggtatta atatcaaaag tgaatgcgct tatgaaaaga
      361 gtaaatacag taaaaaatta tactctatta gtttgtgatg atttattctt ttattatata
      421 gaaaataaat taattataag aacagatgat aaggaagaag agataatttt aagtaagaca
      481 gagacaaaac tattaaaata tttgatggaa aattctatgc agacattgac taaagaacaa
      541 ctgttagaat ctttatggga tagtagtggt aattttgtag atgataatac gattgcagta
      601 aatataagaa gacttagaca aaaggttgaa aaaaatccat ctgaacctaa atatataaaa
      661 acagtaagag gggttggata tatgtgggga gaaaggagta taaaaaaatg ctaaaaaata
      721 tgaacctcaa agatggttta agtgtgaaag atgattatat aatgagaaga gttattgtat
      781 attctgtttt atctgtacta atagtaaccc ttgtatttat actttttgaa tatagcagat
      841 tacataaatt gtatgaacat caaataaaaa atactcaaaa tattgctgga atattgattg
      901 aaaaatatcc taatgatgaa atagatatag taaattctat ttataattcc gattatagta
      961 aggtgtctac tggagaaaag gcatttaaga aatttggcta tggattggaa aataaaatgt
     1021 caaatgataa aaattttagt ttatacttaa aatctttttt taaggaatca ttttttattt
     1081 ttttaatgat gtttatagta gttataggta taatatatta ttttataaga tacataaata
     1141 aaagattgag taaaatttat tttatagtag ataagatggc acaagaaaac tacttgaagg
     1201 acactactga gtcagaagaa actggttatc tagtagaata taatgagtat tttaaagaag
     1261 gtacatttag taagataaat aactgtctat atgagcttaa tagaagttta aagataaagt
     1321 ttattaaatt agaaaaagaa aaagaaagtg taaaatcatt agtaacagat atttctcatc
     1381 aattaaaaac tcctttagca tccttaaaat tatacaatac attacttata gaagaaaaat
     1441 tagaagaaga tgaagccaac gagttcttat taaccaataa ggcttctata aataaattag
     1501 aaaatttaat aaattcttta gtgaacatat caaggttaga aattagcatg ataaacataa
     1561 aaaaagaaga taatgatata aaaaatacta ttctaaatgc agtaaaaagt gttaccccta
     1621 aggctaagag caaaagtata acattaaatt taaatgaatt taacagtaga attataccac
     1681 atgataaaaa gtggactgag gagagtattt ttaatgtatt agataatgct gtaaagtaca
     1741 cacaggtaaa tggagaaata aatatatctg tagaagaaac tgctaatttc tttaaaatta
     1801 taatagagga taatggcata gggattgaaa aaaatgagtt taataatata tttaaacgtt
     1861 tttatagagg ttctgctgat gaaattgaat ctgtagaagg tagtggggta ggattgtatt
     1921 taagtagaaa gattttagaa gaacaaggtg gaaatattat agtttcttca aaagtaggga
     1981 tagggagtaa gttttctttg tttcttacaa ctatgtaata ttaaatgaaa ggaaaatgta
     2041 agcctctaca tttattatat aagtataaac agctccagaa ataaaaaatt aataaaatat
     2101 ggggtaatat aacttagaat gaggggataa ttttatgaaa atactatata ctgaaaattt
     2161 atcaaagcat tatggaaaag gagaaaactt agtaagagct ttggacaatg ttaacttaga
     2221 aataaatgaa ggtgaatttg ttgcaataat aggaaaatca ggttctggaa aaagtacact
     2281 tttacatatg attggaggtc ttgatatacc tacatctggg aaggtgtata tagataataa
     2341 aaatatattt acattaaaag aagaagaact agctgtgttt agaagaagaa aaatagggtt
     2401 tatatttcaa agctataatt taattccatc tcttaatgta tgggaaaatg tagtgttgcc
     2461 aatcggtcta gatggtagag aagtagatga atcttttata aaagaacttt tgaaatcatt
     2521 aggacttgaa aataaacatg acgttttacc aaatacatta tcaggtggtc aacaacaaag
     2581 ggtggcaata gcaagagcat tagctacaag accagctata atacttgctg atgaaccaac
     2641 tggaaaccta gattctaaaa catctgatga ggttatgagt atattaaaat ctatgtctaa
     2701 gaagtacagt caaactctag ttatgataac tcatgatgac agtatagccc aaatggctga
     2761 tagagttata ttcatagaag atggcagggt gtcaaaggtt ggtgataaaa atgattaata
     2821 ctactaaaat agcaaaaagt aatttaaaac aaaataaatc aaaaagtatt ctaataataa
     2881 tcactataat actttctact ttaatgcttt cttctattgg aatttatatt gttgatgcag
     2941 gagcatatca aaaagaaaat actataaagt attcaggtaa ttatcaaggt atattagcaa
     3001 atgtagatga aaaacaggct gatatattga gtaatcatgc agatgttgaa ttaactggtg
     3061 aaatgaatgg agtaggtgtt gaaaaactgg aagatgattc aaatatttct ctagcataca
     3121 tgaatgaaga tgctttgaaa ttaaatagtt ttgaacttat caaaggaaaa ttgccaacaa
     3181 aagagaatga aattgtgtta gatagtggag ctttaaaggc acttggttat aaaaataaag
     3241 acttaggtga aaagataaaa atatcttata atgactataa aaatgataaa aaaattgaaa
     3301 aagagttcgt tataagtgga atcttaaaaa ctagcgaaat atctgaagca ggtaaatatt
     3361 attatgctat tatatcagaa ttatatatga gaaatactag agatatgtca caagaagatt
     3421 ttaatattta tgtaaaattt aatgataaaa gtaatttatc aatagagcaa gcaaaagaaa
     3481 aattagataa aattgctaat gatataggac ttgatactat taatactgct gtaaatgaaa
     3541 attatataaa tgctttaaaa ccagatatgg aaacgattat gggtggaggt tttgtaggat
     3601 tagtaattgt attatctagt attttagtaa tatacaatat attttatatt tcaatagtta
     3661 caaaagttca agaatttgga aaacttagag caattggtgc aactaagaaa caaataaaaa
     3721 atatagtgtt taaggaaggg tttatattag ccggaatagc aataccaata ggaataattt
     3781 taggctatgt acttgcaaat attataataa aatcatttat ggatatagat gtaaaatcat
     3841 cacgattacc agttatatta tcggttgtgg taataagctt tatatctgtt gttttatcac
     3901 ttttaaaacc aatgaaagta gcatcaaaag tatccattgt tgacgcagta agatatacag
     3961 gaaataaaat aagtaataag agtaagagaa aaggatataa aaatataaat ctaaagagat
     4021 tatcacatgc caatttagaa agaaataaaa aacgtactta catgacattg gcatcactta
     4081 tacttagtgg aactatattt ataacagtat caactgcttt agaaagtttt gatgctgaga
     4141 aaatggcaag agaacacttt ccttatgata tagaagttag actatctggt tatgaaatga
     4201 acagtgataa aaatcctaaa aataatttga atattttaca aatggataat cctctaagta
     4261 aagatttttt taatcaaata aagaacatgg aaggaattaa aagaatagaa tctgcaagaa
     4321 gtgtaaaaat tggtatggag gactatgatg ttgaatttaa gtatgacctt ttacaaagta
     4381 taaatgaaaa tgatgtaaaa tcattaaaca agaatctaat agatggaaaa ataaatcttg
     4441 aaagactgca aactggagat gaaatagtaa ttactcatgt tgatacagca aaagaaatgg
     4501 gagtaaaagc aggagataaa ataagattaa ctttatatga tggagataaa aagataaaaa
     4561 aagaatttaa agttcaagca atagctatgg gggttccaag ttttggtata gggaaggatt
     4621 ttattgatag aactttaaaa tacgattcaa ctagtagctt gggaatatat acagaagaag
     4681 gaaagtatca agaaatcaaa gatagtatca agaagattgc taaaaataat ggatttttag
     4741 agactgattt tatagatagt agaattgagt caaataaggc tgttatttca tttataaaga
     4801 taatgggata cacgctaaca ggcataatag gtgtgattgg atttatgaat cttgtcaaca
     4861 caatgataac tagcatagtt actagaaaaa aagaacttgg tatgcttcaa gctattggtc
     4921 ttactaataa acaattagtg aaaatgctta attcagaggc aatgtattac acttctggaa
     4981 tgatgatagg aagtatttta tttggaggta tattagggta tattgctgtt atatttctaa
     5041 aaaagactgg attatcttat gcaacatatt ctttgccaat agtaccaatc ttgttgatga
     5101 ttgtgtgtat tcttatagct caatttataa caacttattt gataggacga agttttaata
     5161 aagaatctct tattgataga gtaagataca gtgaataaaa tgcaaatatt tgtggttagt
     5221 ttaactagca aaaagcatat aaaaaatata gttaaagaga ttcataatta aataggttaa
     5281 agcatagtac agagcaaaca atttaaacgg atgtttgctc tgtattttat tatgtaatca
     5341 ataatatatc taatatactg atattattca tgttagaaaa tgtatcggta caattctaaa
     5401 aaagtataaa attgtaccat ataaaatgaa aattttgtat aataatcgta tagaaaataa
     5461 ataggagctg atatatatga ataagaagat taattatgca ccaggaccaa ctgaaactag
     5521 ggaaaatgta agattagcaa gagcagaaaa aacaacaaat ccagatatag atattgattt
     5581 tgttgaattt tataaaaaga catgcgagaa gtttggcaat atagttggaa caaaaaacga
     5641 tgtttacata ttgagtggtg aaggaatatt agggcttgaa gcagcatgtg catcacttac
     5701 cgaaaaagga gatagagtac ttgtaattga caatggtata tatggagaag gttttaaaga
     5761 ttttgtaaca atgtatggtg gggagtatgt gcttttttca agtgaatata ctaaaagtat
     5821 agatattgat gaattgaaag catttttaga tgaggatagt aattttaaat atgcaacagt
     5881 agtgcattgt gatactccaa ctggcgtatt aaatgatgta agcaaaatat gtccattact
     5941 taaagaatat ggaatattaa ctgtagttga ttctgtagca ggtatggtag gagaaagatt
     6001 aagtgtggat gaatctaaaa tagatataat attaggtggt tcacaaaaag ctatttctgc
     6061 accagcagga cttactatag ttggaataag tcaagatgca aaaaattgta taaaaaatag
     6121 aaaaactgat gtaataggtt tttattgtaa tttaaatata tgggaaggct attatgaaaa
     6181 aaaatacttc ccttatacaa tgccaataag tgacataatg gggcttgata aagcacttga
     6241 taatatatta gaagaaggaa tagaaaatgt attagataga catgaaaaga tagcttatag
     6301 tgttagaaaa tctattgagg aatatggtct agaactattt ttagaagaag gatattctaa
     6361 tacagtcaca gctataaaaa ttcctgaaag tataggagct ttaaaactta ctgattatat
     6421 gcttaaaaat tataatacac ttgttgcaac atcactaaat caatatatgg atactatttt
     6481 aagaataggt catatgggag agaatgcaaa tctaaataaa atagagcatg tactaaatgt
     6541 cttggataaa agtttaagtg cattaggatt caaaggaaat gaaaatttgt tgaatttatt
     6601 taataagtat tatttttaat agataggggg atatagtatg gaagttacag ctaagcaaaa
     6661 atttacaacg aaagacatgg tggaaacctc actattaata gcattagtat ttattgcaac
     6721 taaatttata aatataagat taccaatatc tataaatggt gggcttgtgc atttaggaac
     6781 agcaatgttg ttcatatcag caatagtatt tggaagtaaa aaaggtgctt tatcaggagc
     6841 tattggtatg tcactttttg atttaatatc tgggtggact ttatgggcac cgtttacctt
     6901 tatagttaga ggagttatgg gttatttact tggaaagatt gcatgggcaa atggtaataa
     6961 tgggaataat tttttaatca atgtaatagg aatatgtgtc tcatcaattt ggatgttatt
     7021 tggatattat ataacagaag ttatacttta tggtaatttt atagtacctc ttacttcaat
     7081 accaggtaac ttaatgcaag tattgatagg tcttataata gcgcttccta tttctaaggt
     7141 attgaaaaaa tgtataaaat aaatataaag tgaactaaaa tttatgatag cataaattat
     7201 actgtataat attaagatgg agtacataat taaaaaattt tagtatagag gttagttcac
     7261 aatgttatca gttacaagat attcaatcaa aaatgttcgt ttaaagccgt gggtaaagtt
     7321 tatttggtat tttgaaacaa aatcaaatat actgttaaat aataagttac ttcctacaga
     7381 cagtatagat attattctta atctttctga tgttatggaa tataaaattg aaaatcaaga
     7441 ctatactgct agtaatatgc attttaatgg aataagggat aaacatggat ttattattca
     7501 acatggaaat atacgtgtta tagggatttc tttttatcca tttggtctct atccattttt
     7561 aaagatacca atttcagagt ttaacagaca aattgttgac ttagaagcag tatcagaact
     7621 atttgccaaa aaattggaag agtcattaaa tccaacacaa tcagtagaaa aaattgtttt
     7681 atatttagaa gaaatgttat tatccatatt agaagaagat ttaatttcaa ataaatctgt
     7741 aaagcttcta aattcattta tatatgataa tagatatagc aatataaaag cattttgtga
     7801 taatacaaat ataaatatta aaacattgga aagaatctgt ttaaaatata cagggtatac
     7861 acctaagatt ttaaagagaa tttacagatt taaaatggca agtagtcaac ttatttataa
     7921 ttataaagat aatgaacttt ttgattttat ttatgaaaat gagtactatg accaagcaca
     7981 ctttataaaa gaatttaaaa aattttcagg gacgtcacca attaaattta ttggtgagaa
     8041 taagacgatt aaagaaaata taaagtacaa ctatttataa ttatactgtc gattttttac
     8101 aatacctcca atttattttt tgatatgata taggaaaata aattggaggt tttacatatg
     8161 aaaattatta cagaaatagt tgagtttaat gtggaagaaa atttatcaaa agaagagttt
     8221 atagaaattg taaatgactt agaattaaaa tttcattcta ttcaatctgg ttttattgat
     8281 actgagcttt tatatgatga aaagtctcaa aagtggatta tgatacaaca ttgggaatct
     8341 atagaagacc tcaagagtgc ttctagtaaa atgtttaaag attcttcaac tgagaaattt
     8401 agaaaagctt taaagaatca aactgttaag atgaatataa taccacaaat taaaagttgg
     8461 aaattataga actgaatgta aaagatagtt aaatagtatc aaactaaatt agccaaataa
     8521 cattttgtat ttaaattgtt atttggcttt tagtgtttac tttaatatga gtgttatata
     8581 ctagaaaaac caacaaactg ttagagtgtg gattataata atgtcatatt tgggagctaa
     8641 aaggcttaat actgatttgt caatatgtct ttttagcatg ttgaacaggt atattgccat
     8701 gctttcatta ttttattttt ttgccttata ctggactaca tttgcagttt cctaatatat
     8761 ttggctcata tttaaatgcc attatctaaa agtactattg ctgtacgaga ggcaataggc
     8821 attttagaag aaggaggagt tgagctaaag tatacatcat aatctcttaa gttttctttt
     8881 tttgtgtttc ctccaaactc taccatatca gtatttaata tcgttttgta gtttgtattt
     8941 tgaaactttg aaatatcaac tgattgtatt agattagggt ggaaattaaa tataaataaa
     9001 tatttgccct tactgtacac caaaatcttt ttatcattat ccatatgtat aagttcatga
     9061 ctattttgga taaaaatatc actgtatttt gtaagttcga gcatagattt atcaaaattt
     9121 aataattgtt tatactttaa gttgttatct tctgaaaggc tccattgtct tctggcatac
     9181 ttatagctcc aattgttgcc ttctcgtgga aaatcaatcc actcaggatg tccaaattca
     9241 tttcccataa aatttagata tccttcacct gctagagaaa aagttattaa tttaattaat
     9301 ttaataagag atattgctct attgataaca tggttatttg agtttatttc catattccag
     9361 tacatttcct tgtcagctag ccaaaaaata attgttttat cgccaactaa tgcttggtca
     9421 tgggattcac aatatcctat atttttttct ccaggtcgtc tagtagtaag ttcataccac
     9481 atttttccca aatcccagtc ttcatcagat aagttagaga tagtttttat ccaaaaatca
     9541 ggtactccca ttgcgagtct atagtcaaat ccaattccgc cgtcttttat agggatacac
     9601 ataccaggca ttccactcat atcttctgca atacttatcg aattaggttt aatttctttt
     9661 attaattcat ttgcaaattg gagatatgtt atagcttcaa tatctgtatt catactaaaa
     9721 tatttttcat aactatcaaa tgaaactcca agaccatggt tatgatataa cattgaagtc
     9781 acaccatcaa acctaaagcc atcaaaatgg tactcattta accaaaattt tatatttgaa
     9841 agcaaaaaat gtattacttc aggtttacca tagttaaaaa gttttgtacc ccaagcagga
     9901 tgatttccct ttgaacctga gtggaaaaat tgatgttcac ttccatcaaa ctcatttatt
     9961 ccttctaaag tattctttac agcatgagaa tgaactaaat ccaataaaac agcaatcccc
    10021 attgaatgag cagtgtttat gaggtttttt aagtcttcag gagtaccaaa cctagatgag
    10081 attgcataaa agtttgaaac ttggtatcca aaagatgcat aatatggatg ttccataata
    10141 gccataagct gaatggtatt gtatccagct ttttttatct taggaagtat tttttcagta
    10201 aattcattat aagttccaat agattcgctt tcagtagaca tacctatatg acattcatag
    10261 attagtggag aagttatgtt ttttaagtca aaattattgt cagtccagat aaatggagtt
    10321 tttggttgcc atatttgtcc attaaatcca ccaaaatctt tttgaattac tctttttata
    10381 tataatggta tacggtcaaa tgtttttcca tttgcagtaa cttgtacctt tacctctgat
    10441 ttgtgaggta agctattttg accaggaata aatatttccc aattaccaga cccaattttt
    10501 cttagaggat gagattttct attccaatta ttaaagtcac caattagtga caaactatct
    10561 gcatttggag cccattccct atagaagaat ccatcatttg tctgatgaaa tccatagtaa
    10621 agatgagcat ttgcaaaaga actaaaatct ttatagtttt tcaatgtttt tgatttcata
    10681 tcctcataat gtttcattcg cttttttata tcagattcaa atggttgtag gtatacatca
    10741 atatctaaaa ttttaaatct tttttgactt ttcattgtat ttctcctcca taataaatat
    10801 tacatttaaa aaataatatt tatctcatac tatcttctct gtataattga tatatattat
    10861 ataatcctaa taaaaagctt aagccaccaa tgattaattt cataatttac ctccctagta
    10921 tttatactta tatatcgtat tcatcaaaat ttaaaagttt gtttaaaact cttttaataa
    10981 tatcataatc aaagccttta taggaaagat gttgagaaat tttttgatat attttctttt
    11041 tatcttcttt ctttactctt tcataccttt tttttgctag atacatagca ttttcaaatt
    11101 cagtgtcctt gtctaattca gatactgctt cattgattgt acttctattg atacctttat
    11161 tatataaatt ctgcttaatt ctgtttttgc cacatttatt taggttaaca tttgtgttaa
    11221 caattttttg agctaataca ctgtcattta cgagattata acttttaaga aaatctatga
    11281 ccctctcaat tatattttca tcaaattctg atgatagttt ttcttttaat tttttttcag
    11341 attggtcagc atgagataaa atatttaatg ctttattttt agcttttata tacatttcat
    11401 catctaaaat aggttttaat tcttcttcat ttatttcatt cccttttttt aaattaaatg
    11461 tataaacaag ttctttaaat atagcaataa aaaatttatc atctacataa atattaactc
    11521 tatcatcatt tcttttttgt tgttctattt ttgtaattat acccataagt cctccttttt
    11581 tatatgtaat tatattaaca aaagcaacta ataaaattta acaaaaaact tatatttgta
    11641 tgttatattt tatcaaaatt aagacaaaat agtaaaaagt tatttttatt aaaaagtaaa
    11701 gcagaatcat gttaaataat ta
//
LOCUS       DAELSY010000125        11650 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.125, whole genome shotgun sequence.
ACCESSION   DAELSY010000125 DAELSY010000000
VERSION     DAELSY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11650
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.125"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            205..1530
                     /gene="cdeA"
                     /locus_tag="KPG09_002912"
     CDS             205..1530
                     /gene="cdeA"
                     /locus_tag="KPG09_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBG0967503.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GISFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKGRYKY"
     gene            complement(1625..2419)
                     /locus_tag="KPG09_002913"
     CDS             complement(1625..2419)
                     /locus_tag="KPG09_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0967504.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2420..3295)
                     /locus_tag="KPG09_002914"
     CDS             complement(2420..3295)
                     /locus_tag="KPG09_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967505.1"
                     /translation="MRSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3299..3667)
                     /locus_tag="KPG09_002915"
     CDS             complement(3299..3667)
                     /locus_tag="KPG09_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0967506.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3850..4509)
                     /gene="deoC"
                     /locus_tag="KPG09_002916"
     CDS             complement(3850..4509)
                     /gene="deoC"
                     /locus_tag="KPG09_002916"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBG0967507.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(4545..6089)
                     /gene="murJ"
                     /locus_tag="KPG09_002917"
     CDS             complement(4545..6089)
                     /gene="murJ"
                     /locus_tag="KPG09_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG0967508.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILITGIIFIGITSVMSAFLQIKENFIVVGF
                     GSIPYNIVIIISIMLSTVFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKD
                     DSLIKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYSDRLNGFVTGTFTA
                     SIVSVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFER
                     GAFDARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVL
                     DLVLIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIM
                     GVLSYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKR
                     KLHLKK"
     gene            complement(6563..7447)
                     /locus_tag="KPG09_002918"
     CDS             complement(6563..7447)
                     /locus_tag="KPG09_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0967509.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(7738..8214)
                     /locus_tag="KPG09_002919"
     CDS             complement(7738..8214)
                     /locus_tag="KPG09_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBG0967510.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGVLDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQAKDLEEHHHHDKNCGCGCNHHHEN"
     gene            8481..9656
                     /locus_tag="KPG09_002920"
     CDS             8481..9656
                     /locus_tag="KPG09_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBG0967511.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(9737..10540)
                     /gene="proC"
                     /locus_tag="KPG09_002921"
     CDS             complement(9737..10540)
                     /gene="proC"
                     /locus_tag="KPG09_002921"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG0967512.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINNEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(10580..11164)
                     /locus_tag="KPG09_002922"
     CDS             complement(10580..11164)
                     /locus_tag="KPG09_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0967513.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         3890 a         1887 c         1295 g         4578 t
ORIGIN      
        1 ttttcttaag aaacagttat tccttactaa tgcaatatac ctatttttac taatataagt
       61 ttattaatta gcattatact aatttatctt aatatgattt tttgttgatt ttaagctgtt
      121 tatcgtataa ataaaatttt tcttgactat atagttacta tatagcttat tatagataga
      181 gagtaataaa aggaggtaaa atttatggaa aatttattta caagaaaatt cactactttt
      241 gaatttctaa aatttgtttc tcctgcaatt atatccatga tatttatatc tttgtacaca
      301 ataatagatg gcatctttgt atcaacatta gttggttccg atgctcttgc tagtataaat
      361 attgtactac ctataattaa ccttgtttgt ggatttggaa taatgatggc aacgggtgga
      421 ggtgctatcg tttctatacg tatgggtgaa aatagacagg atgaagccaa ctctacattt
      481 tcttttatag ttttgttttc attgattgtt gggatttcat tcacagtaat ctcatatttc
      541 tttatcaaag aaatatctat tttgcttggt gcaacagata agttattacc atattgtata
      601 acttatggta aagttatgat tttatgtaca ccattttata ttttaaaatt tatatttgag
      661 tactttgcaa gaactgatgg aaattctaaa tttagtttat ttctatcagt cattggtggt
      721 gtaacaaata taatattgga ttatgtattt attaaatatt ttggaatggg tcttttagga
      781 gctgcagttg caactgctat aggtattatt ttaacttgtg ttttaggtat tatttacttc
      841 ttatctaata aatctacact caaattaaga aaaccaaaaa ccgattttag acttataaga
      901 gatactatga taaacggttc ttctgaaatg gttacagaat tatctacagg aattacaaca
      961 ttcttattta atgtagtagc tttaaaatta gcaggagaaa atggacttgc tgctcttact
     1021 atagtattgt atgctcattt tttaatgaca tcagtctatc taggattcgc tgctggagtg
     1081 tctccattaa taagctataa ttttggtgct gaaaacagtg ataaattaaa agaaacattt
     1141 aaacattctc taaaatttat atttgtttct tctcttttag tgtttattat tgctttagta
     1201 tttgcaccat ttattgttag ggtttttgta agtccagata acacagtatt taaactagcc
     1261 ttacaaggat taaaaatatt tgcatttgct tttttgtttg ttggtataaa tatatttgca
     1321 tcaggatttt ttacagcatt tcacaatgga aaaatttcag ctattatatc ttttagtcgt
     1381 gcctttgttt ttataatcat aggaatcata attcttcctc ctatgttaaa catgactgga
     1441 ttatggctta cagttccatt tgctgaggtt ataaccatat ttatatctat tctatttata
     1501 aaaaaatata aaggtagata taagtattaa aaattaatgc ttaaaatcaa catttattaa
     1561 aactaataaa cctgctttaa aatacaaatt tacaaatttg atttttaagc aggttttatt
     1621 ttagttataa taagtcattt gcatactctt taattggtgc ttttctaagt aataaaatta
     1681 taaatattat gcaaataact ataattccta cctttgttcc aaataaaata aaaggattct
     1741 gaaatgaata aataattttt tctaaataca atagcatttt atgcatattt ttagttgcaa
     1801 aattaactat ataaattacc attaatatcg gtaatcctat caatatagta atactagtaa
     1861 cttttctaac tctatatgta aaagctccaa gtaatactcc tataaaacct atacatattt
     1921 catttataca tatttgtaca aaataattta aataagttag tggcatactt gatgttgaag
     1981 gatctatcag tgctgatata gatgtccaaa tcatatctct ctttagtaaa actgatagtt
     2041 ctatactgta acctgttata atttctataa ataacttcga taaaaaaatc aaaactatac
     2101 ctaaaataga aatcacaaag cttaaaataa ttaaatctat gacaatagct tttatacaac
     2161 tttttcgatc tgctcttata cttaaagctc ctgaaaagtc tttatttgct atatttactc
     2221 catacacaaa tagcagtata gttatatatg ttaaaatagg attcacaatc gtggataaat
     2281 tagttttaga tgcttctcca aaaatcttga ggataaacac tggcaaaata ttcataacaa
     2341 ttcccacaat cataaatata attatataca atttagtatt tttattaaaa aattttatat
     2401 atggctttaa ttcattcaat tacaattcct ccttcttagt catgttaaca aacatatctt
     2461 gtagccctat gtatcctaca tcaatatttg aatttttaag tattttttca tcattttcac
     2521 taaaatcacc atagtaaaaa taagcaactg catttccaaa tgccttttta ggttttatat
     2581 tttttataca ttctaatttt tctaactctt ctttatttcc agtaatataa tgagcttttt
     2641 gttttatgac atcaatttct tcatctataa taatttttcc ctcatttaat attattacat
     2701 gctccagtaa atcatctatt tcatcaatca aatgagttga tattattatt gtccttggat
     2761 tttttatata actatctaaa attatgttat aaaactcttg tctatttact gcatccaaac
     2821 caattgttgg ctcatcaaat atggtgatag ctgaattaga acaaatacct attatatttg
     2881 aaagtatagt tttcatacct cttgacagtt gtttatactt ttttttagta tttaaatcaa
     2941 aatatttgca taattcttct tctaagcctt tatcataact tttataaaaa ctagaagaaa
     3001 attcaaatat ctgacctact ttaaaatctg gactataaaa ttctttttcc ctgacgatac
     3061 aaacttcttc caaaactttt atatcttctt ttgggttctt tcctaatatt tctatctcac
     3121 cttcattttg aattaactga tttaccatta tatttaataa tgttgttttt ccagctccat
     3181 tcttacctaa taaaccatat atcttatttt tttcaaagtt tagagacata ttattaaaaa
     3241 tattttgctt cttaaaactg tgagataaat tttctatctt aatagaactc ctcatatatt
     3301 aatcctcctt gaaattaatt ataatatcaa ccaattcttc tctatttatt tctaatttgt
     3361 tagcttcttg aagtaggttt tttacaaaat tatgtttaaa attttctttt ctagcttctt
     3421 ttataatagt ttttgcacca tctgatacaa acatccctat ccccctcttc ttatataaaa
     3481 tatttttatc aaccaatata tttatacctt ttaatactgt agctggatta attttataaa
     3541 gctttgaaag ctctgttgtt gatggaacct gttcttcttc ttttattcca tttgatagta
     3601 tctcatcttc aattgctcgt gctatctgaa taaatatagg ttcttcatta tttaatacta
     3661 atttcatagg ttcctccttt ctccattagt tagttactca actaattaac tatataactt
     3721 ataatatctt gaaaaatatt ttctgtcaat actaaataaa aaacagaggg aaaattccct
     3781 ctgttttttg ttctttatat ttataattaa attttgattt ttgtaatcct atcccataag
     3841 ttaaaaactt taataatctg ttgtatcttc attttttaat attttacata ttgagctagt
     3901 tcctaacctt tctgctcctg cctctataaa tttttctgca tcagaaattg atttaactcc
     3961 ccctgcagct tttattttta cattttttcc aacatgctct ttcataagct ttatgtcttc
     4021 taaagtagct ccacctgttc ccattccagt agatgttttt ataaaatctg aacctgacat
     4081 tgttactatt tcacacattt ttattttttc atcttcatct aaataacatg tctctataat
     4141 gacttttaat actttatctc ctatagcttt ttttatttct tttatttctt ttcctatatt
     4201 atcataatct ttatttttta tatctgaaat gtttacaacc atatcaactt catctgctcc
     4261 attttcaata gcatcttttg cttcaaatac ttttgtagca gtagtttgat atcccaaagg
     4321 gaaccctatc actgtacata tctttatttt accttttaga tattctgatg ctcttttcac
     4381 atatgatggt ggtatgcaaa ctgatgctac actcatatct acagcttcat cacataaaat
     4441 tttaatatct tcccatgtag ttgtagcttt taaaatagta tggtctactg tttttaatat
     4501 atgtttcata aaatcctcct aaatttataa ttaatataat tagattactt ttttaaatgc
     4561 aattttctct ttgcaatatt cagtatcata tcaacctctt caactttaaa aatagtcatt
     4621 agtagtgtat aaattccacc tccaactata actgaaatag ctagtgatac aaactcatta
     4681 aatgttccta ctcccaaaat tccaaatata aatttatacg taaagtatga taaaacaccc
     4741 ataattaaag atgcaactac cgatttcaaa gttgctttta ttattttatc ttgtccaaaa
     4801 tatccaactt ttctctttag gttcaagaag agtaacaata tacatgcaat tgatgatgaa
     4861 ctagttgcaa gagcaagacc accatgagcc attggtttta ttaaaactaa gtccaatact
     4921 atatttaccc ctacagatat aatcccattt accataggtg tttttgtgtc ttgtaatgaa
     4981 taaaaaacct ttcccaaaat atctcttaat ccaaatgcag tcataccaac tgcataaaaa
     5041 attaaggcag ttgctgtcat ttgggttgcc ctcgcatcaa aagcacctct ttcaaaaatg
     5101 attctaacaa caggagttgc aaaaaaaata gaagcaactg aaataggaat catagaaatt
     5161 attatcatat ttatactaga tttaactgaa ctagtaaact tcttttgatt attttctgca
     5221 cttaacttag ataacatagg atacataaca gatactattg aagctgtaaa tgtccctgta
     5281 acaaaaccat ttaatctatc tgaataagta agtgctggaa tgcttccttt aactaatgtt
     5341 gaggctaaag ttgtatctac caatgagttc acttgattaa cagcaacacc tataaatact
     5401 ggtgaaagta atgctagtaa tttaataagg ctatcatctt taaagtttaa gtagtataag
     5461 tatttgtagt tcgttttttt aacaaaaaac atgtaaaaga gcagttgaac taccatagca
     5521 accacagcac caattggaag tatgtatggt ccaaatacgg tacttaacat tattgaaata
     5581 attattacta tattataggg tatactccca aatccaacta caataaagtt ctctttaatt
     5641 tgcagaaatg ctgacataac actcgtaata cctatgaata ttattcctgt tatcaatatt
     5701 ttagtaaatt ttacggtaag taaaaacctc tctccttcaa atcctatagc aaatatactt
     5761 acaagttgct ttgagaaaat aactcctaat atagctacaa ctatgcatat accaactatt
     5821 atatttaaaa cattgtttaa aaactttagt gcttgctttt caccttgttt actacttata
     5881 tcttgataca ttggaataaa agtagtaact atagcagtac ctattgctgc aaatataata
     5941 tttggtatat tcatagctgt taaataactc tctgtatata agcctgtacc ataaattgat
     6001 gataatacta attctctacc cattccaaga atcttagaga ttatagtaac tatcatcaaa
     6061 taaaaagttg ctttagctac cttgctcatt ttatcactcc acttctaaca tacctctttt
     6121 taatcaagta aaatttggct cattagatat aattctatca actaatttgc cttgcttagc
     6181 cctatcaaaa tataattaac acctatttct taatatatca caaactacat tattcttcat
     6241 atttttttat ttctccaata agttttacat ctttcctata taaattgtat agcttcaatt
     6301 atattcaaag aaatggagtt gctacccgat aactttaaaa gtaaaaaaca gattttatta
     6361 tctttttaaa ctatattcta acatattttg tccatattga gaaattttac taaactatta
     6421 tgtactttat taaataaata aaataacagc acacctataa aatacaagtg tgctgttcat
     6481 aaacagattt tataaaaaaa taattcctaa gtagagatta acatgctttc tcatactctc
     6541 atatataatg tgtctaaaaa tattaataaa aatgctttaa ttgttttaac ttacttggat
     6601 gtcttaattt tcttattgct ttagcttcta tctgtcttat tcgttctctg gtaactccaa
     6661 atacctttcc aatctgttct agtgttttag ggtcatcatc atcaattcca aatctcattc
     6721 ttaaaacttg ctgttcttgc tcaccaagac ctgtaagtag ttcttctatc ttttctttta
     6781 aattatgctg ttcaacttcg tgtattacaa catctttaaa atcagcatct gatggtataa
     6841 attcaactaa actactatcc tcatcttctt ttaatggagt atccaaagat acaacatttt
     6901 tatctcttct taataattcc aacactttgt caacttctaa gccacatgca tctgcaatct
     6961 catctatagt tggctctctt aataatacat ttaaaagttc ttgtttcttt ttctttacaa
     7021 aatttattct ctgatgaaga tgtataggta tcctaatagt attttcgcaa tcgtcgatat
     7081 atcttgttat actttgtttt atccaccaag ttgcataagt gctaaatttg tatccttttt
     7141 tataatcgta cttttctaca gcttttataa gtcctatatt tcctgcttga attaaatcca
     7201 acatatcaat actatctctt ttatatctct ttgctatgct cactaccaac ctataatttg
     7261 aatttataaa tttttctttt gctactgaag atgagttgat tatttcctta gccagctcta
     7321 cctcttctcc tgctgacaac attttgtatt cctctatttc ctttaaatac attttcatcg
     7381 catttgatac atttgatgaa cccacacaaa tataattttc ataattattt tcttttttag
     7441 tatccataat caaacccctt ctattcatag aatttgctaa aacaaaaata aataatttaa
     7501 acctacataa tctacaatta cataatatcc cattctttaa atgaattgtt ttgtatatta
     7561 tgtatacaaa tttataaaaa ctatacctat aatattattc tacttctaac cgcaatatcc
     7621 tccttatata tataatttat tttattatat gttgttatat tcttagtttt ttgaatgata
     7681 tatgtctaaa aaaagactgt ttactaaaca gtcttttttt atatatatgc tcaatattta
     7741 attttcatga tgatgattac atccacatcc acaattttta tcatggtgat gatgttcttc
     7801 taaatctttt gcctgttgtt ccatttcatg aaactctttt accattatat cataagttgg
     7861 aagagctgcc tcagtatcgt attcttcccc tctccaatca gcgtacatca tagcatcccc
     7921 attagttaaa accaatctac ctgacatagt tatattatcc acttcttcta aaaattctat
     7981 ttgtttttgt aaaacttctc cttctaactc tacatcttca tctaaacatt gcactatcat
     8041 tacaggtgca taataatctt cttcatcttt aacatttaca acaatatcta aaactccttc
     8101 tgcaccttct tcaaattctg ccaactcaac atttgtatca atcatatctt gagggactaa
     8161 taaatcttct attaaatatt ttattactct atcttttact aattcttttg acatttattt
     8221 ttctccttcc gattctataa cattatcata atcatttcta tattatagta tactacatta
     8281 attgtttttt caatctctta caaaaattat ttttttatta tcttccttat attccctttt
     8341 ttattacaaa gttgtaatcg cattgattta aaaaatagtt tatatttagt ataatagaaa
     8401 tgtagcacat atttttattc aatatattaa taaaaaaata gattgaaaat gtaatgtatg
     8461 ttaaatctag gaggaatatt atgaagttta aaaaacaaat tagttctatt gttgtgtcta
     8521 ctatgcttgt acttgggagt atgaaccttt cttatgcaga tggtacaaat gtagttacac
     8581 ttggagctaa cttatcagag tctcaaaagc agcaaatgct taattacttt ggagtaaaaa
     8641 aagaccaagt cttagtttta gatgtaacaa atgcagaaga aagacagtat ttacaaggtg
     8701 ttgcaacaga gggtcaactt ggaaaagtta ctatctcttg ctcatacgtt gaacctacta
     8761 aaaaaggaaa tggaattaat gttaaaacag caaacttaac ttgggtaact agttcaatga
     8821 ttgcttcaac ccttacaaca gctggtcttg aagatgcaaa tgttattgct gctgctccat
     8881 atcctgtaag tggtactgga gcattgacag gaatcatgaa agcatttgag gatgcttcag
     8941 gaaagaaact tgatgaaact aagaaagaaa ttgcttctga agaattggta gttacaggtg
     9001 acttaggaga tgatataggg caagaaaaag caacaggtgt aatgaatgat ataaaaacag
     9061 aaattgtaaa gaatggtaca aaagatacca atcaaatagc aaatacaata aataatgttg
     9121 taaataatta caatattact attacaccag aacaaaaaga acaaattaaa ggtgtaatga
     9181 aaaaaatagc tgaccaaaat tatgattaca atagcatgaa aaatacacta gataatgtat
     9241 cagataatgt aaatgaacaa ttaaagaaat taggtgaaag tgttaagggt tctggtatac
     9301 tagatactat tggtggatgg tttagtggaa taggagactg gttctctggt ttattctcag
     9361 gcggagataa aaaagatttg ggaatcttaa ataacactaa tgatgagtct cttggctctg
     9421 atgctgtaat aaattctaca gaaggtgttc aaataaatcc acaaaataat actaattcta
     9481 acaatgatag tgatacaaat agctcaaaaa atgatacttc aagtaataat gaaaataacg
     9541 atagttctca aacaaatgaa aacaacacaa caaataattc agaaaatcaa tcaaataata
     9601 atgactcagt tggtttcttt gaaaaaataa aaaattggct ttcaggctta ttttagaaat
     9661 taaacaaata aaaagaattg cctaaaatgg aaattttcta taaaaggcaa ttctctttat
     9721 ttacaataaa tttttattac tcactcattt ttttagattt ttctatacat gcacatattg
     9781 cttttatcac agaagctcta aaaccttctt cttcaagtac tttaactgct tctatagtag
     9841 ttccaccagg tgaacatacc atatctttta attcaccagg atgttttcct gtctcaagta
     9901 ccatttttgc agaacccata acagcctgtg atgcaaactt atatgcttgt ggtcttggca
     9961 ttcctgcaat tactgctgca tctgctatag cttctataaa taaaaataca tatgctggtg
    10021 cagaaccact tgcaccaatt acagcttcta taagatactc tggtactacc tcagtatttc
    10081 caaaagaatt aaatacttct gtaactgctt ctaagtcttc attgtttata tttttattta
    10141 tagaaagaga agacatggct tcatttacaa gagctggagt atttggcata gttctaacta
    10201 tttttttatc ttctcctata attgactcta tatcctttat agattttcct gctgctattg
    10261 taactataat tttattgtca tctataaagt cttttatctc ttccaaaata tcatcatata
    10321 tatctggttt aacagctaca attactatat cagaggttct agttacttcc ttcgagtctt
    10381 ttgtagtatt tattccaaaa tttgcatcta ttctatcgag agttccttga ctatatgctg
    10441 atgctataac catattaggt tcaactaatt ttgaatttac tattccccct atcatagcgc
    10501 ttgccatatt tcctgcacct atgaatccaa tttttttcac tttagtacct ccttattgac
    10561 taatagtaat cgttaaaatt taagcttctt taactgcatc agacttttca aatggatttg
    10621 gtatacttac taccaaaatt ttcactacct tattagtata gttgacccaa ttatgattgt
    10681 tcttttcact attatagtgt attgtgtctc ctggatacat ttctatttgt tcatctccca
    10741 aaaacactgt aagagtaccc tctaatacat acacaaattc ctcaccttca tgtacgtaac
    10801 aacaaatttc ctcactactc ttacttggaa gaatttcaat taatcttggt agcattgttt
    10861 tttcctttaa actagatgat aaatgataat gtataaatgt tgagttttct acatcaaata
    10921 cttctttttc ataacttctt aatacagctc ttttatgctc ttttggcttc ataaaaaagt
    10981 aagttaattc tacatctagt acacttgcta tttttcctaa ggaatctgtt gccacacttg
    11041 ttaatcctct ttcaagttgt gataaaaaac caattgacaa gtttgttttt tcactcatat
    11101 ccttcagcgt catttgcttt tgagttctga gttgctttat ttttgctcct atgtccttat
    11161 tcaaaaccat cacccctaaa taattttaat tttttcataa tataaataca aaaatataaa
    11221 tataggtact gaacctatct ttatttcatt taaaaaaaat ttttataagt attactataa
    11281 tttatcaatc tttttttgtc tacattttta cgattattta cgatttattt ttcacatatg
    11341 tatatttaat cttgccaact aaagatacat tttttataag catttactct ttatataata
    11401 aaacttaagt tgttcatgta gtcttcttct ggtttaatta ttaaaatttc aaattatatt
    11461 attatttcta cacattatta catcctttag tacaattata acataatttt acaatttaat
    11521 gatatctatt caatgtcttt ttttactcta caacatattt tattttagag tttaatacta
    11581 tatatatttt ttatatacag ccttgtcatt ttttagattg tcaagagtta ttgaagtatt
    11641 ttatgtagat
//
LOCUS       DAELSY010000126        11590 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.126, whole genome shotgun sequence.
ACCESSION   DAELSY010000126 DAELSY010000000
VERSION     DAELSY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11590
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.126"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            197..895
                     /locus_tag="KPG09_002923"
     CDS             197..895
                     /locus_tag="KPG09_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967514.1"
                     /translation="MKISSQYRSQYSFRHESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINEAIKNLYKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            997..1545
                     /locus_tag="KPG09_002924"
     CDS             997..1545
                     /locus_tag="KPG09_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967515.1"
                     /translation="MEGLGFYAAALSGSSYQRIGFGKLDPIEVIADGDWISYKQAQDT
                     LTVIRNFLNSFDWRNASEMERANRAAKLVTEAKYVDSKYCNIVYGNLVDKRGVCGSFA
                     SSFHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRSDGSEISAFGGALDFDYRKLK
                     DATREMTTYYDAKALSILGFNQ"
     gene            complement(1946..2902)
                     /locus_tag="KPG09_002925"
     CDS             complement(1946..2902)
                     /locus_tag="KPG09_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0967516.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDNNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSYGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIQFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENKSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            3605..4600
                     /locus_tag="KPG09_002926"
     CDS             3605..4600
                     /locus_tag="KPG09_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0967517.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKTSVIVLKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            4578..5441
                     /locus_tag="KPG09_002927"
     CDS             4578..5441
                     /locus_tag="KPG09_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG0967518.1"
                     /translation="MSIMSNYNEASSNYSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            5438..6232
                     /locus_tag="KPG09_002928"
     CDS             5438..6232
                     /locus_tag="KPG09_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG0967519.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISKLNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            6517..7896
                     /locus_tag="KPG09_002929"
     CDS             6517..7896
                     /locus_tag="KPG09_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0967520.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKELLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            8351..9064
                     /locus_tag="KPG09_002930"
     CDS             8351..9064
                     /locus_tag="KPG09_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0967521.1"
                     /translation="MDTYFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSAVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(9336..10874)
                     /locus_tag="KPG09_002931"
     CDS             complement(9336..10874)
                     /locus_tag="KPG09_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG0967522.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVEEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
BASE COUNT         4226 a         1309 c         1734 g         4321 t
ORIGIN      
        1 tatgaattat aaaatacaaa taagatattt ataaaaatta ttgttttaat ttataataaa
       61 aattttttgt tgaaaacaat gctcgaacct ataaaactat taactaatat ttgtaaatgt
      121 atacttttga tgataaaata taattaagtt aagttggttt ttataaacat aaatttattt
      181 tagagaggtg aaataaatga aaatatctag tcaatataga agtcaatatt catttagaca
      241 tgaaagtaat ataaataata caagaataaa tgaaagtatg gttaagaaaa atgaaactgt
      301 aggaaaagac acttatttat ctaatattat gaaacaaaag caagaactta atgatagaat
      361 tagagattta aaatatagac aagaggttta tactaaaaaa ataaatgaag caattaagaa
      421 cttatataaa tcagaaataa gagaaacaac taataacttt tctaatatag aaataggtat
      481 taaaaatagc attatagaag ataaaaataa aagtacaatg ttagatgaaa attcaactta
      541 tctaaataca aatgatgaaa aagaatcttt aatcactaaa gagtctaatg aaaaaattga
      601 agaagaaata ttaaatgatg aaaaattaga agagttagaa caaaaaaagg attataaaga
      661 ggattctaat aaaaaagaga aagtatctga ggacttatct ttagtaggta aaactcgtga
      721 agagcttgaa aatatgctta aaaattttat aaatttaaca caagaagaaa taatgaaact
      781 tgagtcgaga atagaaaagt tagataaaga tgctgaagaa tacaaacaaa attcaaagac
      841 taatatattt gataaaacag atgaacaaaa aaaacatata aatgtactga tttaaacaat
      901 atgttttaaa aaaataagtt taatttactc tatggaggaa atccaaatgg agttgagctt
      961 cgagcagatg gtaaatatta ttatataaat aagggtatgg aagggctagg attttatgca
     1021 gcagcattgt caggtagctc atatcagaga ataggctttg gaaaacttga ccctatagaa
     1081 gtaatagccg atggagattg gatttcttac aaacaagctc aagatacact tacagtaatc
     1141 aggaattttt taaatagttt tgactggaga aatgcatctg agatggaaag agctaataga
     1201 gctgcaaaat tggtaaccga agcaaaatat gtagactcta aatattgtaa catagtctat
     1261 ggtaatctgg ttgataaacg tggtgtatgt ggaagttttg caagtagctt tcatttatta
     1321 acaaggttaa tgggaatgga ttcactatct atattaaatc catcattaaa tcatgcttgg
     1381 aactatatac aaattgatgg gaaatggtat aggtctgatg ggtctgaaat atcagctttt
     1441 ggtggagcat tagactttga ttaccgtaaa ttaaaggatg caacaagaga gatgactact
     1501 tactatgatg ctaaggcatt gtctatatta ggatttaatc aataaaaata ggggaggctt
     1561 tataaagagc ctttttttat tgatttgaaa tttaataagg aattttatat gaaaatttaa
     1621 tcaataaaag caaaaaaggc tctttataaa gcctttctaa gtgttttttt aagtaaaatt
     1681 ttttatatca acatcagttt taatcaaaaa atataacatt ttggacttac ttttaaaaat
     1741 aaaattatta aaatataata ttaatcatgt taataatgtt tttagtttat tatattctat
     1801 aatagtggtc atgttgacta ttgtagtttt attaagaata gttggtttat ataaatatat
     1861 gcaaagatag cttgttgttt actttactta ttagtatata ggacaagata ctctaataaa
     1921 tttgcctatt ttaatatctt ttgtactaaa accaactaaa gtttccttta ttatttcgtt
     1981 ttgaataata caaagcataa tccgattgtt tgtataaaga ctggaaagta tcagatttat
     2041 tttctaattt agatatacct atagaacaag aattactctc tagacataat tctaaaactt
     2101 tctttgcttt attttcaaca ttctcttttg attgattact atattcaaaa acagcaaatt
     2161 catctccacc aagtcttcca ataatagcat catttacgaa tactttagtc aaaatatttg
     2221 aaaactgtat tagtatttca tctcctcgaa catgaccaaa gatatcattt acctgtttaa
     2281 aattatctaa atcaaagatg taaaagtatc catatgaaac agttttaagc atttctttta
     2341 tgtgtacttc aaaaaaatgt ctattgtata gtccagtcat aaggtctttt tctgatttta
     2401 gtttaaattc atttgccatt gctagtgctt cttgtgcttt ttcagttatt gttttagggt
     2461 aatactcatc tttcatttgg tcaaagtttg gaatagaatg gtgaacatgg ataagtttaa
     2521 tcatatcatc tgtaactctg tataccatag taaatcgagt atccatttct acatagatat
     2581 ctttttctag gtaatctttt tctattaccc aaagagtacc atatactata aaagcttcgt
     2641 ctgtaacttt ggtacatgaa taccactcat ctataatctc aaagtctatt tgctctgatt
     2701 ctgtttggtc tttttcaaga cctataataa attcttctaa tgtttcgtaa aattcatgtt
     2761 ttcctgttcc aattaatgca aggttgttat ctaataagga aataataaat ttaaggtttt
     2821 cttcttctgg atttaaaata taaatatgcc aaattttttt tgataaatct cttaattgtt
     2881 tttgtattgt attattttcc atagattacc cccttaagat ttacagttta tattttactg
     2941 tattataagt tgaactctta ttacaatatt attataatat agaactaaag aaattacatc
     3001 ctgtataggt aaaagtatat cttttacaat aaaaatatta cagtaaaata accagtttta
     3061 atatacgtat tatattttta atttataaac gaatattata aatgaaattt aaattcaacc
     3121 ctgaagaaat aagctaaatg tattagtata taaatccata cttgaacata aataaccttt
     3181 atgatatgat atcaataata atattatgag aggagtgcct tgatgtcgga gttatataat
     3241 ggtaaccaac aattaaatag ttggagacac atatattctg tataatcaga aattttaagt
     3301 gtatttttaa tacatttaat ttttgtgtct tattctgcct ttatagataa ctgacatatg
     3361 acaacaaata gtatttaaaa tatatattac tttgttaaat tatggtgtag taggctttaa
     3421 tctgctacac ttttattaat tttgtatagt attattttga taactatttt gaaatagaga
     3481 gcattaattt ttctagtcca caggagtttt ctgtttttgt gctgttaaat ataagcacag
     3541 aaaaaggcag atatatacct gtggactttt tttatataaa ttatgaaata tggaggtagt
     3601 taaaatgagt ataattgaac ttaatgaact gactaaatct tatagaagat ttgagaaaaa
     3661 ggcaggattt atggggagtg taaagtcttt atttaatagg aaatatacag aaaaaacagc
     3721 tatatcgtca tttgatttaa agatagaaga aggagaattt gttggattaa taggtcaaaa
     3781 tggagctggc aaaactactc ttattaagat gttaactggt attattgcac cgtcttctgg
     3841 agaaatatct gttatgggtt attaccctaa caaattagaa aataatttta aaaaacaata
     3901 tgctgttgtt atgggccaaa aaagtcaact attctttgaa ttgacaccag cagatacgtt
     3961 tcttttattt aaagaattat atgatatatc taatgaagaa tataaaaaaa acttggatta
     4021 ctttatcgaa ttatttgagg tatctgacta tttaaatgtt caagttagga cgctttcttt
     4081 aggagaaaga atgaaaatgg aattaatagt agcccttcta cataatccaa aaatattatt
     4141 tttggatgaa ccaactatag gcttagatgc tatagcccaa aaacaaatta ggtatttcct
     4201 aaaagaagta aatgaaacaa aaggaactac tattatactt acttctcatt acatggaaga
     4261 tattaagagt ctctgtagta gaagtattgt tgtagaaaat agccgtaaaa tatatgatgg
     4321 ttctacagag aaactattta atcattttca aaaatataaa aaaataacag ttagtttttc
     4381 caatgaaact aacattgtat ttccaaaaga ttgtaaagtt gttgaacaaa caaatcataa
     4441 gacatcagta attgtgctaa aagaaagagg aaatgaaacg attaaatgga tttttgataa
     4501 atatgatgtt aaagatgtaa tgattgaaga agaagatata ggtagtattg tagagagaat
     4561 atataagaaa ggaagtgatg agtattatga gcaactataa tgaagcaagt tcaaattatt
     4621 caatgtttta taaatttaaa caaagtatta gaaggtatta tgaagtagcc aaaattaact
     4681 ttaaagtgca acttatctgg agatttgatg tggtggttaa tatggtgttt acgattgtaa
     4741 aaattttatt tgcttatatt ttatggaaag caatttttgg tgaaaataaa atagtctctg
     4801 gttttacatt taactctatg ttgtcttact atataataag ctcattttta gcaggtattg
     4861 atatgtcaaa gagtaccagt gaagagatat gtaatcgtgt aaaattgggc acattttcaa
     4921 agtacatgat tattccaatt aatgttcaaa attattttat tgctaaaaat tttggaatga
     4981 catgttttta ttttgtattt aatatgattt ctgcatttgt atggattttt atatttaaaa
     5041 tagattttgc ctttacatca aatccagttc aaatattaat ttcaattttg atgatttttc
     5101 tagggtttat ttttatgatg aaactccact tctttattgg gattttgtca tttaaatttc
     5161 ttgagattag tttttttaga atgataattg aaaatattgt tgaatttatt acaggaacaa
     5221 tgataccact tgtactctta ccagaaacta tagtaacagt tatgaaggta tttccatttt
     5281 attatacaac atatttgcca tctatgttac taataggtag aaacagcaat gaggcaatac
     5341 aaggattaat tattctagca ttatgggttt tatttttttc tgtagtaaat aaagttatgt
     5401 ataaaagaat gagaacatta tttgatgggg tgggtatatg atgaaaaaaa atttgaaaat
     5461 aatatttatg ttattaaaaa tgaaattatc aaagatgatg gtatttagat ttgatttttt
     5521 tggagcattt tttgtagata gttcattatt tatattacaa cttctaatgt ttaatagtat
     5581 atattctcat gtagactcaa tagggggatg gcaacaagga gagatgttaa tatttatagg
     5641 aactttctca ttaataaatg cgataaatat gacgattttc ttttttggta tttatgatat
     5701 tccacgaaaa atccaagaag gagaattaga ctactatatt acaaaaccat tagacccact
     5761 atttaggtta acatttgaaa atattaatat tggttcatca ccattaattc tagcaagtat
     5821 acttattgtt ttatatggaa tatctaagtt aaactcagag ataactttag ttatgataat
     5881 aatatatatt ttcatggtgt tattgatgac attacttttt tatgatgtat gtatcatact
     5941 tagaacagtt tcattttttg ttatatcatc ttctgcgata atgagattag aagataattt
     6001 attgccaatg aatatgaaaa ttcctggtgt aatatatgaa ggtgttttta aagtattatt
     6061 ctatttaatc ttaccatatg gaattatgtc gacaattccg actcaaattt tggctgggac
     6121 aataacaatt aatggattaa tatactcaat tttaattgta tttttgttta cattgtttat
     6181 gtggagattt tggaagttag gtatgagaca ttataaaagt gcaagtagtt aataaattag
     6241 taaaaataga caatgtataa ctatatataa atacgtttca tatgacacta aactatgtga
     6301 acaagtattt gcgctaaagg cactgaaagc tataaaatga ttaaagtaag gatttaaaag
     6361 tttctttagt agaaatataa ttgtgtaaaa aagcaagatt aattaaaact atatccaaat
     6421 aattagatat aattcttatg cagaaaggag gtctgtaaat gtctaattgt ttggaatata
     6481 ttttaaaaat aattaatgta cagaaaaata aacaaaatga gtgatgtata ttattcagaa
     6541 aatcatgaac aagataatct ttatgagcaa gataatttac tggcacaaag atttaattta
     6601 tttactctat tgaagtttgc atttcctact atttttatga tgatttttat ggggttgtat
     6661 acaatcgtag atactatatt tgtatcaaga tttattaata caaatgctct atcagcaatt
     6721 aatattgtat gtccaattat taatcttata gttggaattg gaactatgct tgccactgga
     6781 ggaagtgcta ttgtagcacg taagatggga aatggtgata ttgagggagc aaataaagct
     6841 ttttctttaa tcgtattatc aggagttgta attggggtaa taattactgg tataggtatt
     6901 gtatttatgg attatatcat tatatggtta ggagcaagtg aagttttaat taaatattgt
     6961 cgttcatatc tttttattat cttggttttt gcttctgcaa atatactaca agtattattt
     7021 caatgtttat ttgtaacagc tggaaagcct caatttggat tatggctagt gttaggtgct
     7081 gggtgtgcta atataattct tgactatgtg ttcattgttc tgttacaaat gggtattagt
     7141 ggagcagcac ttgcaactgg agtaggatac ctaattccta ccgtttcagg tatggttttc
     7201 ttttcaaaaa ataaagagtt acttaagttt tgtataccca gactagataa aagaatatta
     7261 tttgaagctt gttttaatgg ttcatctgaa atggtaggtc aactatcaac agcagttact
     7321 acttttttat ttaatgctat gatgatgaaa ttaattggtg aggatggagt tgcagctatt
     7381 acaataatta tttattctca atttctttta acttcgcttt atataggttt ctcaatgggt
     7441 gttgcaccaa taataagtta taactatgga agtgaaaata atacacagtt aaatcatgtt
     7501 tttaagattt gtatattatt tatttcagtg gcatctttat tggtatttat cttttctata
     7561 tcatgttcac caatcattgt agagattttt tctcaaagag gaagccgagt ttatgaaatt
     7621 gcaaggtctg gattttatat attttctttt agttttattt tttgtgggta taatattttt
     7681 acttcgtcta tgtttacagc attgtcaaat ggcaaggtct ctgcaatatt atcttttcta
     7741 cgtacatttg gatttataac tataggttta ttgatactgc caaatatatt aaatataaat
     7801 gggatatggt tagcagtacc aagtgctgag attttaactt tatttttaac cattttttgt
     7861 atatgtaagt ataagaatag atataaatat atgtagatat taaataaata gtttaggtag
     7921 atattttatg ttttaataag taattcaatt agtggtagat atatactatt gaaaattaaa
     7981 gttataattg atataaagaa ttaataaaaa taacatataa aagtttaaaa gggagttgaa
     8041 cttaaagttt gattagaaaa agtcaatttt aagacaactc cttttatttt actattgtat
     8101 taatctgtat ctgtatctgt aagtgtatta gtctaatatt ataaactttg gatgaaatta
     8161 attttctata ggttttaatg tatcatacac tttattttaa agaaacttat cttaattttc
     8221 atagtgattg aatttgtcta ttagagatgt tactattagt aatgtaaaag caagagtagt
     8281 gaatataaaa aaacaagata ttgatactgc aagattagaa tataataaat ttttatggag
     8341 agatttcaat atggatacat attttaataa aaaaatatta cttgttgatg atgaaaaaga
     8401 tatagtagat ttaattgaag aagtactcat aaatgatggt tttaaaaaca ttattaaagc
     8461 ttataatggt ttagatgcta tttcgctgtg taagatagca tgtccagatg ttgtcattct
     8521 tgatataatg ttaccagata tagatggtat agaagtgtgt aaaaaaattc gagagttttc
     8581 atactgttct attctttttt tatcatcaaa aaatgatgat attgacaaaa tactaggtct
     8641 tagtagtggg ggagatgact atataacaaa gccattcagt ccaagagaaa ttgcttttcg
     8701 agttaaagcg caattacgta gacaacagta tcaaagcatt gtaccatcag atagtgcagt
     8761 tattaaaata ggagatatta ctattgatat agaaggaaac cgtgtatata aagatagaaa
     8821 tgagattgag ttaactggaa gggagtatca tcttttaagc tacatggctc aaaatgtaaa
     8881 taaaattata ggaaaagaaa gactctatga acaagtatgg ggagtatata gtagtatttg
     8941 tgacaataca attatggtgc atatacgaca tataagagaa aaaattgaag ataacccctc
     9001 aaatcctaaa atacttataa cggttaaagg actaggatat aaacttgtga atagaatcga
     9061 ttagaataga ttttaatatt aatgaagatg tctcaagttg aatttttagt taatgaggca
     9121 ttcttttatg tgtgaagtca aatgtatttt cgttatctca acaatgaaga agaggtctat
     9181 ctctattttg agatagcctc attaagttta tatcctggta atgtacttta tgttctaata
     9241 aaattttttt agtttatttg cagtgaaaca ttaactagaa cattaaaatt tgcattgaaa
     9301 tatcaattaa ataatattgt atattgtgta taaacttata ctaattgttt ttctatatta
     9361 aacactttat caatccaacc tttataaaat ttttcttcat gggaaactag gattatactt
     9421 cctctaaact gtttcaaagc attttgtaaa gcatcctttg tctctgcatc aaagtgattg
     9481 gttggttcat ccagaataag aaaattacaa ggagataaca tcatacaaca taatttaacc
     9541 ttagattgtt caccaccact taaagtagat acactccttg aaacatgctc ttccttcaca
     9601 ccacatctgg ctagatgacg tctaatctct tttgtattta attttggata tttatctgct
     9661 actatttgaa gtggggtttt atcaggattt tcccatttta aatcttgctc ataataacca
     9721 attttaactt gttctgaaaa ctggaaatct ccagatatac gaggaatatc tttgactaat
     9781 gtctttaaca aggtagattt accgattcca ttaaaaccag ttataactat tttttgacct
     9841 ccatcaacag aaaaatttaa ttttggaagt aaagaatagt agtatccaac ttcaagattt
     9901 gttatagtaa gagcattttg agccgatatt tcaatttctg aaaattgaat atttggtttt
     9961 cctgtaaaac taggaggagc tattctttct attttatcca attgtttttg tcttcctctt
    10021 gcaatttttg aatttactcc agctttattt ttacgtataa atgtctcttc tttttctatt
    10081 ttcttttgtt gtgcttgata ccttctaata taatcttctc ttaaatgtga tttttgttta
    10141 aggaactctg aatattttcc atgatatttt ttaatcatac caaattctat atcaagaata
    10201 cctgtagaaa ttttttctaa aaaatcaaaa tcgtgggaaa ctacaataaa tgccccatta
    10261 aatgtattta aataatttga gagccattca acatgttctt tatctaaaaa gtttgttggt
    10321 tcatctaata atagaacatc atggttagaa agtagcaatt ttgctaaaat tacctttgcc
    10381 ctttgcccac cactaagctc ttcaacaaca cgattcattc ctattgaatc taatccaaga
    10441 ccatttgcta ttttatttat ttcattatct atagaataga aattacttgc tattagttgt
    10501 tcctgataat ctgatgcttt gattagatat tgctcatttt cagatatagc acttttttga
    10561 tagagtaaat tcatttcctt ttctatttta taaagttctt caaatgcagt gtgtaaatag
    10621 agtgatattg ttgtatctct attgatttct gcatattggt ctaaatggcc tatattgata
    10681 ttaggttgcc acttgatttc tcctgaatct ggaacaactt ctcccaataa aattttaatt
    10741 aatgtacttt tacctgtacc attttggcca actacaccta tatgttcacc tttgaataaa
    10801 tcaaagttag ctttctcata cagtgattta tccataaaac attgtgataa attagttact
    10861 tttaataaac tcataattca aactcctttt cttttattaa aaaatttatg gaaataaaaa
    10921 aagcagagga atggcattgt aaaatgccga cctctgcttt tggaaaaaca aaaggtcata
    10981 gacaagcatt gttgtctatg accagaaaat ctatactatc tatcggcaaa taggtaattt
    11041 taaataagca tagccaaaaa actatactaa acctatattt gccgatgaag atagaaagct
    11101 ttaagctttc agattaacct ctacacaacg cttcatcgta tacatttgtg tagagttaag
    11161 ttttctaaca agtttaacta attctatata aatcataatt attcacatcc taattttagt
    11221 attaattcta gtttatctat atttaaattg tacttttgta ctttgtggat tatcttattt
    11281 atttaaaata tgttttatta attatagctt aagttatgtt atcataatac ttggttatag
    11341 tcaataagaa agttatattt gctatatatt atattaattt agtaaatcta tcattacaaa
    11401 gttatttgaa gactcgctac caataaaatt ttatagtcta tgggaaaatt aagattatga
    11461 gttttatact tttgatatag ggatactatt ttcataaata ttacaacatg tagaagtata
    11521 tgtctagtat tgttgaattt tcaatgatat attaaggttc taatatatta tacaaaataa
    11581 aaacattaat
//
LOCUS       DAELSY010000127        11551 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.127, whole genome shotgun sequence.
ACCESSION   DAELSY010000127 DAELSY010000000
VERSION     DAELSY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.127"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            415..567
                     /locus_tag="KPG09_002932"
     CDS             415..567
                     /locus_tag="KPG09_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967523.1"
                     /translation="MKIFSFLLFVVGICFCIAGFMGHYPAFAGATLSFFATTMIAVRQ
                     VKTKKK"
     gene            complement(859..1563)
                     /locus_tag="KPG09_002933"
     CDS             complement(859..1563)
                     /locus_tag="KPG09_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0967524.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(1554..3161)
                     /locus_tag="KPG09_002934"
     CDS             complement(1554..3161)
                     /locus_tag="KPG09_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBG0967525.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(3415..4593)
                     /locus_tag="KPG09_002935"
     CDS             complement(3415..4593)
                     /locus_tag="KPG09_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967526.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(4819..5313)
                     /locus_tag="KPG09_002936"
     CDS             complement(4819..5313)
                     /locus_tag="KPG09_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG0967527.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(5314..6075)
                     /locus_tag="KPG09_002937"
     CDS             complement(5314..6075)
                     /locus_tag="KPG09_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG0967528.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            6798..9632
                     /locus_tag="KPG09_002938"
     CDS             6798..9632
                     /locus_tag="KPG09_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0967529.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHVSIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNISKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNIRKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            9675..10919
                     /locus_tag="KPG09_002939"
     CDS             9675..10919
                     /locus_tag="KPG09_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG0967530.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKV"
     gene            complement(11360..>11551)
                     /locus_tag="KPG09_002940"
     CDS             complement(11360..>11551)
                     /locus_tag="KPG09_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HBG0967531.1"
                     /translation="VGSKATYKTAIDEAQAILDKADATQKEIDDAVTALNTATATFEA
                     GKVPTTIALMLSRILGFMK"
BASE COUNT         4131 a         1852 c         1432 g         4136 t
ORIGIN      
        1 tataaaacta ttatatatta aaatttcttc aataataaat tatctagatt gttactttga
       61 tatattatat aatatcacaa aggctgatat aaaaagacaa aaagggtaat ttttttatat
      121 caaaaaaaat gttaaattga tattatccat atataaattg atattatcca tatagatgct
      181 atatttaaca attctactta cttcccttac acttattagc atatagttat taagagtttg
      241 ttgcaaaaca acacttagca cactttaaaa agtgtggtct aataagtaat caacgaagaa
      301 gacataataa agcataaaat atgatatact tttcctgtca tcaaaactga tgattttact
      361 attaatatct gctaaaatat gcttaataaa ttaaatttta gtgggagaat agttatgaaa
      421 atatttagtt tcttgttgtt tgtagtggga atatgttttt gtattgctgg atttatggga
      481 cattacccag catttgctgg tgcaacattg agtttttttg caactacaat gatagctgta
      541 agacaagtca aaactaagaa aaaatgaaat aggcaaatcc taactttaca agttaatatt
      601 acataagtaa ttagtctaaa attggatact ttttctatta cagaaataca tcatccactt
      661 tgtattctct taaaaaaata aaatagctat gtaaattcaa gattagatag attaactctc
      721 tatccatttc attgtaaaat atacatagct atttgtattc gatttaaaat acttagattt
      781 tcttacataa caaataattt ctatctgatt attactcata ttcatttagt aatcactcag
      841 caactaatca tttagtatct attcatccgc catacggtaa cctaccccaa cctcagtcaa
      901 aatatattct ggctgagcag gattcttttc aattttcctt cttatacttg ccataaaaac
      961 tctaagcgac tgattttcac tttcatagta atttccccaa actttatcta ttataaactt
     1021 atgagttaag actcttcctg aatacttaga catcaactca ataatcttat attcgattgg
     1081 agttaaatgt atttcttcac cattaattga cacaatatga ttttcataat ctatctttag
     1141 gtttttaact tcaaaagact ttggagcatc attatttata ttatcctgtt gagctttatt
     1201 tctaagagcc acacgtactc tagccaaaag ctctgttaca ctaaaaggtt tagttaaata
     1261 atcgtctgct cctccatcta aagcttccac cttatccctc tcatgctctc tcgctgaaac
     1321 aacaattatt ttattagtct ttgcacatgc cctaactcgt cctattacct caatcccatc
     1381 aatatctggt agtcctaaat ccaaaattat caaatctggg tcaagagaat gtataagtga
     1441 tatagctgtt cctccattat ctgtctctac acacctataa ttttgagttt caagagatac
     1501 ttttataaat ttacaaattg gcttatcatc ttctacaatt agaactaatg gctttatttc
     1561 cacttgtctt tcccctcctc atcatacata ggtatgctaa atctaaatgt agctcctcca
     1621 gaaggattat taaatgcgac aatttctcca ccatgagctt ctactataga tttacatatc
     1681 tccaatccaa gaccaatacc tcttctttca agatttctag acttattctt agtataaaac
     1741 ctattaaaaa tattaccgat atcttcattt ggtattcctt ttccattatc tgcaacttca
     1801 aatagcactt gatttgactc tttttttaca gttagttcta tcttagaatt tttaggtgta
     1861 tgccttatag cattatcaat taaatttact aacacttgtt ctattaataa tacatccaca
     1921 tgaacgatta ttatctcatc tggaatattg gtttttatgt ctctactatt ggcaaacttt
     1981 ttgactctta atatagattc agctattatt tcatcaacaa cttctggtcg tttttcaata
     2041 tccaatttcc cttcatctat tctagtcata ctaagtatat tctcaacaga atgagttagc
     2101 caactcgtat cttcatatat atttttcaat aattctactc tagtttcttt atctatcaca
     2161 tcatcatttt caagtattgt tgaagacgaa cccaatatac ttgtaagtgg agttcttaaa
     2221 tcatgagaaa ttgacctaag caaatttcct cttaatctct ctctctctgc ctcaagattt
     2281 gcttttttat ttttctcaaa taaattttct ctatcaattg ccaacgcaac ctgtgttgaa
     2341 actgcttcta atagtatctt ttcattttct gtaataatat ctatcttaaa acacgcaaca
     2401 cctataacac caagaacatt tgattcttga ccttttatag ggaaataata tgcactgctg
     2461 tttttgcaat attttgtccc aattccaact gggttacctg tcctaaaagc ttcttttata
     2521 gcttcttttt ctaatactga atggaatata ttctcacttc catcattgtt acaaatataa
     2581 atacttggct tacctagatt attattctca tctgcaatag tcactattac agttctattt
     2641 aatatattta ctaaattaga cccacaaaat tcaataattt gatttttatt ttttgcttta
     2701 agcaacgttt tgttattctc atagagcaat cttatcattt tttctctctt atgagaaagt
     2761 ctcacttctc tttttatcct agaagttaaa gtacttgtta taattgctgc cattagcata
     2821 atacaaaaag tcataggata atcttttcta taagctaaaa aagtatataa tggatgcgta
     2881 aagaaaaaat taaatgatag cactcctatt actgaagaaa gtattccaaa aaaatatcca
     2941 taggttatac ttgtagcaaa tagtacacct aatatgtata ctagtataat attagattca
     3001 ttaaaaccta ccttttcaaa aactactgat attccagtag caattgtcat aaccaatata
     3061 gtttttatga agtccttaat aattatcttt ttactgagat agccatcttc atcataatca
     3121 tactctttta agtcttcatg ttgtagtcgt tcctttttca tttactacaa ttcctcctta
     3181 ttgttactgt ttaaattggc tctattttat ataatacaat tttatcataa taaattcaat
     3241 caatacattt aattaatgta cttaaattac taaaaggtgc tgtcttaaaa tgaaaataaa
     3301 tctctatttc aaaacagcac ctatatattt aaattactaa tttagttttt acatacttaa
     3361 atcaatagtt ttattcaatt ttataaatta taatacttca attatggaac atttctatcc
     3421 aacaagcata aatataaagt gggcacatat acctgcacac aaaccaccta tggtatgcga
     3481 aagtatcgct ttaccagcca atggtcttgc atctaaagca tccatcatac ctatgtgtgt
     3541 acttaaatat ccactccaac acatacccat tgctgtgaat actgctatat cattaggagt
     3601 tatagcgcct gattttataa actctggtac taaagatatt gctgctccaa cagctcctaa
     3661 tgcagttaca gggaatgcta ttgcctctgg tgatgtgaat ccaaataatg gttctattat
     3721 gaagctaatc ttatcaccaa tagcaggtaa taattttata ccttcatatg cagctcctgt
     3781 gtaaacagct tgacctgtag cagggtctgt agatggtcca aaagttaata acataactaa
     3841 tgtacaaact actaaaactc ctggtattat agccataccc atttcaacac ctagcttacc
     3901 accttcaagt atagcatcca atgttctttg gaatacatta ccatctctta tttctctaaa
     3961 ctcttcaagt tttgcacctt tatcatttat ttgcttttca gtatcatttt tagggtcata
     4021 attataatac tttttagtaa aagtcaacat aattctaaca ctaataatac tacctattat
     4081 tgccccaaca ttacctatta tagctggtag tacatattct ttaccttgag ctatcataaa
     4141 agttgttaca ataagtccca taccaaaggc tgttcctaaa ttacataaag caggtacttg
     4201 atacttctta aaatattgtg taaatgtttt gtctttagca aatggtataa tagctggatt
     4261 atcagataaa tatgttgcaa ctactcctgc tatactagca ccaggtaagc cccatatagg
     4321 tctcataaga cccttaaata tcttattaac taaagatatt actccaaact ctgaaagcaa
     4381 cgcactaagt gcacctgcaa gaactgccat agccattatt aaaaatactg tgtctaataa
     4441 aagcgcatgt gctgtactca ttattacttt aaacatcatt cctgcaccca tgatgcttcc
     4501 tacatatcca aatcctaccg ccaatagtac aataaatacg aaggtttcca tacttactgc
     4561 cttgactctt ctcttatccg acagcttttc catcttgaaa tcttccccct tttaatttta
     4621 aattaaggtt cctaacattg gtacaaattt tttggtacaa actgagttct aaaacttaca
     4681 ctcgtaatgc aagttgtcag ttttttgaaa taaaaaaaaa ttacgtttat agaataagaa
     4741 tatcctcttt aatttggata ctcttattct attttatgtt attatattat catccttttg
     4801 tttaaaagtc aattttttct atttttgttg tttaatttta tttatactct ttttatattt
     4861 ttcattttct tttatctttg taagcactcc atataagttg acttttctaa atacagtagt
     4921 tataaatacc aatcctaatg caattgaact tgcacatgat aatgtactca ccccatattc
     4981 tacagctttt atataattat tggtcaaaac agaatacata gtattatata ttccataacc
     5041 aggtactaat attattaaac aacaaacact taatgtagtt gctggagtca tataaattct
     5101 tgcacataac tcacaatata tcgcaaaaca aactgtagct ataaaaaaac ataagttttc
     5161 tgttactccc atatttaaaa gaaatttata tgaaaaccaa gctatacttc cacctattcc
     5221 tgctagtatt aagttttttc ctttcatatt aaatagcact ccaaagctta aggcagtaaa
     5281 aaaagctgct attacttcta taaacaaatg cacttatatc ccccctaact tgaagtataa
     5341 tccaagtacc aagaacattc ctactgctat agatacacct acaaaaaata cttctgctaa
     5401 ttttgcaaga ccagaaatca actgtccatc taacaaatct cttaatgcat tagtcaaagc
     5461 aagcccggga actaagagca ttattgctcc tgctattaaa tggtctaacg tctttataaa
     5521 tccgagcttt aaaattaaaa ttgaacaaga agctataata actgctccaa ttgcatttac
     5581 aaaaaagtca ttaagtgaag atttacttaa atacataata acaaacttag tcagaatccc
     5641 aacagtaaat gcagctataa attcttccac atttcctcca aataatatcg aaaaagttgc
     5701 tgctgcaata cctgcaaaaa atattgttat taaatccgag taatagttat cttcacttat
     5761 ttcatctaaa attttctcac ataattcaat atctaaatta tttatactta ttgctcttga
     5821 taatgagtta acttcatgaa ccaagtttag gtctactctt cttgagctta ttcttttaat
     5881 tgctgaatgt aatttcccat ctacaaatac agatgccatt acagctgttg gagttgcaaa
     5941 tacatctacg tggtctattc caaaagattg acatattcta gctatcgttt cttcaactct
     6001 ataggtttct cctccacttt gcaacatagc tttacctgca tttgaggaaa agtttaaaat
     6061 tctatctata tccacaaaac atatctccca ttcttttata gtattattca tattttaata
     6121 caagatatta tttatttaaa ctattttaaa taattcatat ccaatattta cattttatga
     6181 aaaaacttgt tttttatgct tgtcaaaatc aaattttaag cttgtttaca ccataaaatg
     6241 gctgaattaa tatttgacaa acactttcaa ccatttatat aaccttctaa attgtataac
     6301 tttttactac aaataaaatt atatctcttt taattctttc tcttgagtat ttaagcttat
     6361 agcaacttca tatttatata tgtacaatat aaaacattat ttttatgata caaaatataa
     6421 tttgtaaacg tatatatgta ttaactgtgt atatataaat tattcttaac cttatcagtt
     6481 ttttcatata tcttcatcta tatcgaccat atattaatta cattgaccat atactaataa
     6541 atgatattac atagactgaa gtatattaaa aaacttaaat ttagttattt aacaacataa
     6601 tactctaatt taatctataa aaatccatac ctaatataat gcaactctta ttttaacacc
     6661 atctattaca caaaacaaca tattaaaaag taaaatacaa aatatccact ataaacctta
     6721 ttttaatact gaatatatat tataatatat ttaatgtagc ttaaatatag tttaaacata
     6781 aagaaaggaa atggtatatg ctaagaaaag ataaagttct attatctcta cataaattat
     6841 gtgaaaatat gactttaaaa gaccttcaat cagataaaac aggattcact acaatagaaa
     6901 ttgctaacca ttcatctctt gatagaagta ataccagtaa agaattaaat aacctatata
     6961 atgaaaaaaa agttctgaaa atatctggta agccaatttt atttctacat gttagtatta
     7021 tagaaaattt aattggccgt actttgaaca gtgatgaatt ttctataaat aattgtaatg
     7081 agctacttaa atcaagtaaa aatgactcag aagatatttt ttcaacatta cttggacatg
     7141 attctagtct taaactacct atagaaaaag ctaaatctgc tatactctat cctccaaaag
     7201 gtcttaatac gttactcata ggacctactg gtgttggaaa aagtacattt gccgaaacaa
     7261 tgtacaaata tgctgtagaa agtaaaatct tttcttctga aagcaaattc gtagtattta
     7321 actgtgctga atatgcagaa aatgctaact tattattaag taatttattt ggttatgtca
     7381 aaggttcttt tactggtgct aataaggata aattcggcat agttgcacag gctgatggag
     7441 gcatattatt cttagatgaa atacacagat taccaccaga aggtcaagag atgctttttc
     7501 ttttgatgga caaaggcata tacagacgat taggagaatc tgacattgtg cacaaagcta
     7561 atgtctttat tgtgtgtgcc acaacagaag atatacattc ttctctgctt gggacttttt
     7621 taagaagaat ccctgttaca atcaacctac ctgctttaca ggataggtct ttaaaagaaa
     7681 gattatctct tattaaatac ttctttatta tagaatcaaa atacaccaat gcaactataa
     7741 aagttcacaa agatgtaatt aaatcattac tcctttatga ttgttttggt aatataggtc
     7801 agcttaaatc tgacatacaa cttatgagtg caagagcctt tttaaattac aaaactggac
     7861 ttaaggataa tatagaaata gatttatccc ttgttcctga ttacatctat attggtcttt
     7921 taaaattgaa tgaaaatcgt agtaatctaa ataaattatc agaacttgat tatatgcttt
     7981 tttatgaatt taatggtaat aacttagaag ttgaatatac aaaagaacta tctaaataca
     8041 atatagatac atcaaacaat ggatttaaat actctacaga gattccttat ctggaaaatg
     8101 atataaggag tatgtttata aattacatgg aaaagtactc tacaaaatta ctttcgatat
     8161 cttcagtaac tcattctgat tatacaagta atgaaatttt taaagtaatc aatccaaaga
     8221 tttactatgc tgttgaacat tctttaaaag aggccgaaaa aatatcgaga aacaaataca
     8281 caaaacaaac ttatattgct ctttcaatgc atataagtgc acttatagaa aatataaatc
     8341 aagacaccta taatcctaag tacaatttat ataatgataa aattttctta gataaagatt
     8401 taaatatatc taaaatagtt ttaagtataa ttgaaaatga cttacaaaca tctttaccta
     8461 aagatgaagt aaaatttata tccatgtttc taaatagcat gtatcttgaa ccaaacaatc
     8521 aatctgaaag tataggagtc ataatacttg ctcatggaaa ttcaacagca agcagtatgt
     8581 gtgatgtagc aaattcattg cttggtacaa accattgtaa tgcaattgac atgcctcttg
     8641 atgtaaaagt aagtaccata cttgataaaa ctatagagtt ggtaaaagaa tgtgacgaag
     8701 gtaagggtgt acttctttta acagatatgg gctctcttat agcttttgga gaacttattt
     8761 cagaaaaaac aaaaatcaaa actcgctcaa ttgaaatggt atctactcca atagttttag
     8821 aagcagtaag aaaaagtaca ttaccagaaa tgactttgga tgaattagct aattccttaa
     8881 gctctttaaa tccatatatt ggacgagcag caatcgattc aacagaaata aactatcctg
     8941 aaaagtcaag agagtatgta ataataacta cttgtataac aggatatggt tcagctatga
     9001 aagtagcaga ctttataaat tcttcactta aaaatattcg taaatacaat attaatttag
     9061 ttcctcataa tatagaaagt tttaaaatgt ataagaataa atttagtaat actaaaattc
     9121 tggcagttgt tggaagtgta gattttaatg tagccaacac cccttttata cacatagagg
     9181 aattactaaa tggatatggc ctagatatgc taaacaaaat aatagaaggt acattcaatg
     9241 ccaatagtat tacaaatata aaagaaacaa atctatcata caacagttta ataataaact
     9301 ttatgaccca aaacttagaa cttttggact ctgttaaatt attcaactat gtcaattctt
     9361 ccttatttac aataatagaa ttaatagatg aaaacttaaa gagcaaattt aagctaggat
     9421 atatactaca ttgctctttt atgatagaaa gatgttttaa aaatacaact tttccatata
     9481 ataacattac tgaattaatt agtaaaaatc atgaactata tagtttaata cgtgaagcct
     9541 ttaagccaac agaagcttat tttaatattt tgataccaga cgaagaaatt gcttatataa
     9601 tagacatgat taatgagtat ctaaataaat aaaatttata gcacttattt tgtgcatatt
     9661 aattaatata tgaaatggag gacgagattt tgaaaggaaa aataaaacag ttaacaatac
     9721 ttgcattaat ttttatattt ataactcctg tatttgcatt tgcagatact ccaccagtac
     9781 caaattcttc aagagcagca ctacttatag accaggaaac taaaagaata ttatttgaaa
     9841 aaaacattga tgaaaaaatg ccattagcga gtttaagtaa aatgatgacc tttttattgg
     9901 caatagaagc agttgataag aatcaagtaa aagagactga tatggtgaaa atagacaagt
     9961 ctacagcttc agtaggaggt tctacttgta aacttaaaga tggtgatgaa atatctctag
    10021 gagagcttat gcagggtcta atgctagtat ctggaaatga tgctgctata gccatagcta
    10081 aacatattgg taagactgaa aagaattttg taaatatgat gaacaagaaa gcagaagaaa
    10141 taggaatgat tgatacgtat tattttaatc caaatgggct tcctatatat actgacccag
    10201 aacataaaga acctcctatt gaaaatatgt caactgctca cgatatagtt acacttggaa
    10261 agtatatgta tgaccattat gaaaatcaag ttacaagaat aaccactatg caagtatata
    10321 atgatactaa aaaggatttt acacattata acacaaatcc cctacttgtt tcagtacctg
    10381 gtgttgatgg aataaagaca ggctatacag ataatgctgg atattgcctt gcattttcta
    10441 tgatggtacc taaagatgct aaaaacgaaa gaaatcatag attgataggt gttgtacttg
    10501 gtgatggtaa caagaaaaat agaatttctt cttccgctac tttattgaaa tatggtaaag
    10561 ataatttcca ttctaaaaag atagcacata agggtgatat tatagagact ccttgtgttg
    10621 atggtattga tgattttaag ataacagtta aggttgataa agatttgtat ggtgttgttt
    10681 cagataacga aaatatcaat cctaaggtag tgtttaaaaa tatgaattat cctatccata
    10741 agggagatat tgttggtgtt gcaaagtatt ataatgactc tggtaaattt gttggaagtg
    10801 ttgatgtaaa aagtgaaagt aatataggat gtattccttt gaaggataag attaagatta
    10861 aggttgctaa gataaataaa aaacttgaga ttaaaaattc tgtttgtttt aaagtatagt
    10921 aaagcatact attaaaagag tatattaaat ttataaatac tccactttgt ggagtttttt
    10981 tgttttttac aaaaaattaa aatagtggca acaattgagg atgtgaggga gagttgccac
    11041 tattttgtat agggtattat attatgtttt taagctagtt ttactatctt ctatgtttat
    11101 ataataactc ttgtgtgtga atttaattga taattaatat gaaaattatg tgaagttttg
    11161 atattaggtt atactttgaa ctttatctaa cattttaaat tttatatctc taaattatct
    11221 aaaattacta tatcaattaa ctttacatga taaaaaggct gtgtataaat ttatttttaa
    11281 aaattttact cacagcctta aattaaattt tctatttaaa gtatatataa attttattaa
    11341 ataatttgct ttttatttct tatttcataa atcctagtat tcttgataac attaatgcaa
    11401 ttgtagtagg tactttccct gcttcaaatg tagctgttgc tgtgtttaat gctgttactg
    11461 catcatctat ttctttttgt gttgcatctg ctttatctaa tattgcttgt gcttcatcta
    11521 ttgctgtttt atatgtcgct tttgatccta c
//
LOCUS       DAELSY010000128        11468 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.128, whole genome shotgun sequence.
ACCESSION   DAELSY010000128 DAELSY010000000
VERSION     DAELSY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11468
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.128"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(257..682)
                     /locus_tag="KPG09_002941"
                     /pseudo
     CDS             complement(257..682)
                     /locus_tag="KPG09_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
     gene            complement(1155..1442)
                     /locus_tag="KPG09_002942"
     CDS             complement(1155..1442)
                     /locus_tag="KPG09_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967532.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(1462..1737)
                     /locus_tag="KPG09_002943"
     CDS             complement(1462..1737)
                     /locus_tag="KPG09_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967533.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAIGMLKGVDYEPLIVATQVV
                     AGTNFKFICNATSVTNPPHDYLAEIIVFKPLPCDEHNATITSINIIK"
     gene            1925..2744
                     /locus_tag="KPG09_002944"
                     /pseudo
     CDS             1925..2744
                     /locus_tag="KPG09_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(3003..5570)
                     /locus_tag="KPG09_002945"
     CDS             complement(3003..5570)
                     /locus_tag="KPG09_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG0967534.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTNQLEDVSKKIGELFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMDGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIALQSEKE
                     LWGLSEWVKQNNSINLYLQNSPAIKIAEDFMSSVMPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAYSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     APQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(5573..6178)
                     /locus_tag="KPG09_002946"
     CDS             complement(5573..6178)
                     /locus_tag="KPG09_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0967535.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPSDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(6552..8012)
                     /locus_tag="KPG09_002947"
     CDS             complement(6552..8012)
                     /locus_tag="KPG09_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG0967536.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDRAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            8343..8921
                     /locus_tag="KPG09_002948"
     CDS             8343..8921
                     /locus_tag="KPG09_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0967537.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIKRHNLKLKIEKEKAFGVCNS
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            9213..10463
                     /locus_tag="KPG09_002949"
     CDS             9213..10463
                     /locus_tag="KPG09_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0967538.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            10624..11193
                     /locus_tag="KPG09_002950"
     CDS             10624..11193
                     /locus_tag="KPG09_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG0967539.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVDFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAVAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         3770 a         1996 c         1610 g         4092 t
ORIGIN      
        1 ttacctgctg attgcccatt ttaaagtact tagggtttaa ccttatacca tctaaccaat
       61 tttttctact ttcgtaactt tcacacttag gtttatttca tccttatgtt ttagtttggt
      121 tagctttagg ggtttccagc aattcaagta gtattggata ggctctatcc tatctctaca
      181 tataggtttc cctatatgct gactatttta gctactaact catgactaaa ttcacgagtg
      241 tgcgtagcta ttttttaatc aatacaatta ctgcacatat agatgcagaa tttttaaata
      301 cttcacctaa tgaaactgct gcactaatat catgggctac tcccctattg tcgctaaaac
      361 ctgcagtatc acataatttt gcatccattc cttctattgg ttttctcata tttacaatgt
      421 ttgggtataa agtttgagaa acagctcctg aaccaatctc tgccacaggg tctaagcaat
      481 caatatatga tataccagta tcttcatctt ctacttccat cattacacat cctgcaagat
      541 agttaattgc agtacttttg ccacatcctg tcccaccaag aattagaatt aaattttgat
      601 tttcagcctt atttattgct acttttcctt catttaataa gagtgttatt tcttctaaaa
      661 gttgattgtt attttctggc atttattttc ccccttggtt atatatttta tttaaataaa
      721 gtttaatccc ttaagtagat tacaaagttt ttagactata tttaaatttg ttttttattt
      781 ttaataaatt tgcattttca ctcaataatt attcttcttt gtttattaaa ataactgtgt
      841 tgtataatat aatttttacc aacacagtta ttttatttaa tgcgcttatt ttattgtatt
      901 ttatgtaata cagttgtttt tttatattat atgtccacta ttttcctatg gttattacat
      961 atagattttt aattatattt gtaccattaa tttaaacatt cctatactac tgcttaagtt
     1021 ttacacctat accagcttga atattttatt tttatttgct taaatagttc aactatactg
     1081 gtttaatagg tttcctttag tttaaatagt tcacctatac ctacttgaat agttcacttt
     1141 tatttgctta aattctatac caatactggc ttaaacatta tactaccatg attgtattgt
     1201 ctatcttcaa ctacaataaa aacaggttct aaatctttag tagatgttat ttttaattct
     1261 aaaaaaacat tatcagttgg taatattttt acatcataat tatttaaatt attattcgtg
     1321 atactattaa atacatcacc ttgacgttca aaaaaaacat tatcaactat tacaaaataa
     1381 acatcttctt ttctactatt ttcaacctct atagtatttt gtttagttac atttagtctc
     1441 atcttccacc tcctattaat attacttaat tatattaata cttgtaattg ttgcattatg
     1501 ttcatcacaa ggaagaggtt taaaaacaat tatctctgct aaataatcat gtggaggatt
     1561 tgtaactgaa gtagcattgc aaataaattt aaaatttgtt cctgctacaa cttgagtagc
     1621 aacaataagt ggttcataat caaccccttt tagcattcct atagcttcat taaatacttt
     1681 taaatcttct tcttcgacag ccctaaattt tgaccatcca ccaacaaaaa gactcatata
     1741 aaaacctcct ttatatatat atttatttat atgatagtac caattttctt aatattcaat
     1801 atattacgtc taactggtca ataaaaatgc ataactggat aaaaagactt ataaaatagc
     1861 ttaactttac atataataag attattctaa aatatgagac atatcttgct ttgaatatat
     1921 tctatacaac aattttccag gcctcattgc caagtttaag gtaactgact ttggattcca
     1981 acttcttgat gctaataaaa aatattttga tatgttcaaa gataatgtaa attttctatt
     2041 agatgatata aatttagaag gttatcttaa caatgtgtat aaatatcata atgattttaa
     2101 atctgggaga tgcgtttgtt tttctatttc tccacaaact attaatggag atatttttca
     2161 catgcaagtt gtagctgaat gtatagattg ggaagatgat aagcctatat atcttttaat
     2221 atattcagat gtaacgacat tggtaaaaca atatgtctca ttatcatttt tcatccaaga
     2281 attttatcaa gaaatatgat aaaatagcta aaaaatatga tattccttct caccttatag
     2341 aaattgagat tacagaaaca attgtatttg aagatgtcaa agtatttagt gaaataataa
     2401 atgaaattca taaaaatgac tttaaatgct ctattgatga ttttggaagt ggctattctt
     2461 caatcaatat gcttaagggt atttatgttg atacattaaa attagataga gcattttttc
     2521 aatcagaaga tttagattct caacgtgaaa gaaatataat tgtatctata atcgaactag
     2581 ctaaaaagtt aaaaatgacc actgtagctg agagaataga gaccagtttt caaaaagatt
     2641 tcttgcaaga aataaactgt gatatgttac aaggatacgt gttctctaga cctttgcaaa
     2701 taaaagactt tgaaaaactt atgtttcaaa actcctccaa ttaacaatat aaaattgctt
     2761 attataagtg tattattgta tcaacaaata aaggaattgt ctcaaaattg attttttcta
     2821 atcaaatttt tattttgaga cattctcatt tttttgcata agaaaatacc caccaagcta
     2881 cggtttttct taccgctgtt cggtgggtat tagtagtatt atgaaatctg aaaactttaa
     2941 gtattactac ttccgctaac gcaatcacaa aggtcgaaag cttcaaagcg aaaataggct
     3001 ttttactcat caaacgtcac cgtacctgcc aacccgtcca ccgttatcat ttgtccgctc
     3061 ttaatagttc gtgtggcaat gtgtgtagcc ataacggctg ggatgccata ttcacgtgcg
     3121 acaatgcttg aatggctaag aggaccacca atgtccgtta caatcgcact tgcagatgaa
     3181 aataatggtg tccatgctgg agttgtggta actgcaacca aaacacttcc aggttgaaag
     3241 ctttcaaaat ctgctggact attcagtaca caagctggag ccgtcacaac accagtactg
     3301 gtaccaatgc ccgtcaacac aattttccca tctttctgct tctgtggtgc ttgtgatata
     3361 gtttttttgt ttttctctgg caacttggac ggtgacacat aacccatgta ttttttaagt
     3421 tcagcttttc gaacaggtat tatcccgttc atatcagata gagacttgtt tttatccaac
     3481 tgctctataa gtacctccaa ttccgatttt gtgagccaat aaatatcatc taaatgtgaa
     3541 atagctcctc ctcgtatgaa tcgttctgag atttcctgaa acatacaacg aatcagagga
     3601 tgtcccattc ccatcaaata aatagcattt tcacgcatag gagaggtttc ctgtgcccaa
     3661 tggagcaact taaaaaatag ttttttacgc aatccaccta tgtgttgtaa aatctcttct
     3721 tctgcatgtt ttctacgctt ttcaaacgtg gtctggcgta aaaaaggact ttcccctttt
     3781 ccttccacaa atgtttttac ggattcaaaa gttggtgtaa gtgtttcctg cggtgtggaa
     3841 tacgcaaaat caaactcata agcggtacga ccaaactctt taaagtactt attgattcga
     3901 tttttccatt ctatccatac ttcctgtgaa actccaacag gcataactga agacatgaaa
     3961 tcttctgcta tctttattgc aggactgttt tgcagataaa ggttgatgct gttattttgc
     4021 tttacccatt ctgaaagacc ccacaactcc ttttccgatt gcagagcaat cgtatcaaac
     4081 ccaagcagga aaacggaagt atccgttata ccagcacgtc gaactgctcc ttgaaataat
     4141 tttgtaaata atgtttcact gatagaagca gcaggcaatg taagctgaat ccttgtgaaa
     4201 taaatacatg cggcataaaa tattgtctga ataccatcca taatttgatg ggcgttcatc
     4261 gtatgcaggg gcttttcttc ccattgttta atagcatcct catatccttt tcgtgccgtt
     4321 tcccagcgtg caacgctgtt cataagtgtc ctacgtaaag agcgtgggga aagggatttg
     4381 atagcaatca aaagtggctt tgatttagca gaaagataaa catatccatt aataaccgta
     4441 tatgcgccgt tttttggtaa taattttttc gcacttttac caaacatatc tatccataaa
     4501 agctccgatg cacggtttac gatttcaaga ccaagcgtgg cgaacaaagg tgtaacagga
     4561 tttggcaagt gttctgccaa acttcccttt gtgtatacaa catcctgttc tggcaatacc
     4621 cattccggtg gcagaacagt aattggacga gcttgaacaa tgtacaattt atctttttcc
     4681 agtgcccact ctacatccat aggcatttga taatacttct caattttctt tccaagctgt
     4741 atcagttgca ttacctgatt acgagtaaga gcatattttt ttctcaacct ttcaggaacc
     4801 atggtttctt ctgttccttc tgaagtacga accgtcataa tctctttatt tgccacttca
     4861 tacgatataa ttcgttcgga gtccttatcc actacgattg tatcgggtgt aactaagcta
     4921 gaaaccaccg cttcaccaag cccccatgca gcgttaataa tcatttcgct acgtctacca
     4981 ttgattgggt ttaacgtaaa cataatacca gatgaatcgg aaaatgcaag cttttgtact
     5041 acaacagcaa gtgcgacaat ctcgtgcttt atattattct tcacacgata agcaatagca
     5101 cgagctgtcc ataaggaagc ccagcatctc tttacagcgt caataacttt atcttcacct
     5161 tgtatgttaa gataagtttc ctgctgacct gcaaaagaag cgtcaggtaa atcttcagca
     5221 gtcgcagacg aacgaacagc caccgatatg ttccctaatc cggcataagc tgttttaatt
     5281 gcgtccgaca cttcctgtgg catttctcca ttatggaaaa gctcccctat tttcttagat
     5341 acatcttcaa gttggttggt attattggaa tcaataccat ctagcagttt attaattcga
     5401 gactgaatat ggttcgtctc aacaaaaatc tggtatgaag cagtcgtaac atgaaaaccg
     5461 tctggcacag ggatgcctgc tgtcaatagt tttgataaag acatcccttt tccaccaaca
     5521 atttcaatgg ttgcttgttt atgttccagt ggcaagacat aataaatcat aatcattcac
     5581 ctccatcatt cataattccg tgcagaaata tattcattgt ttcatccaac attttttccg
     5641 aactatccga tggtcgtgac gtgtagctga ttgataacat ggagttaatt aacaaacgtg
     5701 tcacaagagc ggtatccgtt ttgcgaaaga tgcctgtggt cacaccatta tcaattaccg
     5761 attttataat atcttgatat gcataacgag catctttaag tctctttcga tactcttctt
     5821 ctaaataatc agattctgta tttaaccaca tcagtacagt tctatactgc tctggaaatc
     5881 taagatgatt aagcatgatt tttctaaggc actgttgtgg tgaaggttca ccatcaataa
     5941 tcctatgaac ttgttggata gttttttcta tttctttctc aactgcatat acaacaatct
     6001 cgtctttcgt tttgaaaaaa tcgtatagac ttgactttcc catgttggct aaaactgcaa
     6061 tttctctcat tgatgttttt tggaatccat tttcagccat gagactcaaa gcaacagcaa
     6121 ctattttttc tcggcgttct gcttgttgtt caggtgtaag cacaattcct tttggcatag
     6181 ggcgatacct ccttttagcg accaatggtc gtttcttttt tatttatcat acagcgacca
     6241 tcggtcactt gtcaaggtta atttttttca ttgtatatgg tactcgagat aaaaatgttc
     6301 attttagaaa ttgctctatt taagtagaaa taggcttcat cattagaaaa caagactata
     6361 aatttgagat atttatcatg ttcaaatttc atcaatatca aagtttaaga tattaataaa
     6421 aaaagaatta ctaagaaata acatataaag attcaaaaaa ggagttgaac ttaaaattca
     6481 attacaaaaa atcaaattta agacaactcc tttattcttt atctctacta ttctttatct
     6541 ctatttattt attacaactc cctaacattt accactactg ccttaggtct agtcatagcc
     6601 tcgattgaat acttaacacc ttgagttccc ataccagatg cctttacacc taaaaatggg
     6661 aagtggtctg gacctctttc agtcttatta tttatttgaa cagtaccaac ttcaagttta
     6721 tcagctatat aaaatgccct atcaatatca cttgtaaata ctgatgattg aagcccatac
     6781 tctgattgat tagcaatttc aattgcttga tttatatctt taactcttat aattggaagt
     6841 actggaccaa atggttcttc ccaagcaatt ttcatatcaa catttacatt atctaacaat
     6901 gttggataga ataaattatt ttttctaaca ttacctgtta ttaactttgc tcctttatgt
     6961 aatgcatcat caaccaaccc ttgaacaaaa tcagttgctt tattatctat aagaggagtg
     7021 ataactactt catccattgg atttccaatt gtcaacttct ctaccaatgg ctttattttt
     7081 ccgactaatt tatccgccac actctcttgt actaaaattc tctttactgc cgtacatctt
     7141 tgtcctgaat atgaatacgc tccagatact atattcttag ctgcaaaatc taagtctgca
     7201 tcttctaata ctatagccgc atcttttcca ccaagctcca ataatagtgg taccattcca
     7261 gatattttag atatatgctg accaacttca gtactaccag taaagttaat aaaattaatt
     7321 cctttatgcg ttacaacata atcccctatc tcactacccc tacctgtaat agtattaagt
     7381 actccatctg gtatcccagc ttttctcatg atttctacca catgaagtgc gctaatagcc
     7441 ccttgtgtag caggttttaa tacaactgca tttccaccaa taagcgctgg agcaatcttt
     7501 gacattgata aatttacagg ataattaaaa ggagatatag ctaaaacagt acctaatgga
     7561 acccttgaaa cataagacat cttatttctt gttccaccag ggaaattatc tccactaatg
     7621 gcttcaccct ccatattttt tcccacatca gctgtgtatc ttaaaaaatc tgctgttctc
     7681 tcaacttctg cccttgcaga ttttatgtct ttggctattt caagtaccag tatattagct
     7741 atctcatcta tattttctaa caataaatca gcagccttat gaaatatatt ggctctcttg
     7801 tatacaggca tttcagccca tacttttata ctctcttttg tgttttttat aacctcatct
     7861 acctcatgtt ttgtcataga ttgtactttc ccaacaagag aattatctat tggtgagtaa
     7921 atttctattg gctttctcga attagattct acccattttc catttattaa atttttatat
     7981 acattttctt ttgttttaag ttcattaaac atatttttat tcctctctct gatataactt
     8041 tgtgtattta aattatagcc atttattcgt tatgtaaaca tttctttttt tcataaacgt
     8101 ttacctataa aaaaaatttt aagttaacgt ttttctagat tcttctattt taataacata
     8161 ctcgctccaa atctatatag gtcacacttt aatatcaata taccaatata ccaatatacc
     8221 aatataaata ttataaacat aaatcacttg acaaatttat aagtagacaa tattatatat
     8281 ctatgactta aaatattttt aaagtcatag atagcatatg cagatagaaa ggattgataa
     8341 aaatggctaa aagttttaca gaaatagaaa aagacaatat aagaaaaaga ctaatttcag
     8401 aatgtgaaat caactggtct aaatatggat acaaaaaaac taacattgat gaactttgtt
     8461 caaaatcagg aatcgcaaag ggttctttct atacattttt tgattcaaaa gaaaagttat
     8521 tttttgaagt actaaatgga atccaaaaca gacttatatc atcagtagat gaaattttaa
     8581 gtaacatgcc tccaaaagaa ggctttgcaa aatccttaaa aatgttatat cgattatatg
     8641 ataaaaatcc atttttatat tcaccaaata atcctgattt catcagccta ataaataaac
     8701 tacctcaaga tatgttttta caacttgagt ccaataacat acaaagtttt tacgacttaa
     8761 ttaagagaca caatctcaag ttgaagatag aaaaagaaaa agcctttggt gtttgtaatt
     8821 ctctactatc aagtctattg gtaaaagaaa atattgaata taactatatt gaagtatttg
     8881 attttatggt agataatctt gtagaacaca tacttgaata acaatttaaa ataatttttc
     8941 taaatatttc tttatactaa agaggattaa tatgattgaa ttaatgaaaa aaataaacaa
     9001 tatcatttac aaaataaatg ataaactaaa tatacacaca aaacttatag accccagagt
     9061 taactcattc cactctactc taagccatct ataatagttc gtatatttct aataatatta
     9121 gatattaaag taagaaaaaa attccttata ataattgcta ttatatcaat tattataaat
     9181 attttaataa tcttagatag gtaggtatat atatggtaaa atcctcaaac acttttaata
     9241 aaaacttcaa cttgatgata ttaggtcaaa taatctcatt gtttggtgca tcaatactta
     9301 aatttgcttt atctttatac atacttgata taactggtaa agcagaaata tttgctacta
     9361 tactagcagt atcttcaatc ccaattatag tattctcccc tattggtgga gctattgcgg
     9421 atatattcaa cagaagaaac ttaatggtta tttttgattt ttcaagcagt cttacagtac
     9481 taatcttagc agtattctta ttcaacaata atggttcaat attacttgta ggaattatta
     9541 tgaccatatt atcagttata agcacaatgt atcaaccaac tgttcagtca agtacacccc
     9601 ttttagttga taatgagcat ttaatgaatg caaatggtat tgtagctgga gttgcatctc
     9661 ttacaaatat tgcaggacct gtattaggag gtgtcttgta tggtgtgatt ggaattaatg
     9721 caattatagt tataagttgt gtaagtttct ttctatctgc aattatggag atatttatac
     9781 agattccttt tatcaaacaa gagcaaaaca gcaacattat aaaaactata tttactgata
     9841 taaaggatgg tattgttcat atatgtaaag aaaatagatt tatactaaaa atactttttt
     9901 tagctatagc aataaactta tttttaagct ctatgttaat agttggtatc ccctatatag
     9961 taaaaatcac tatgggagct agtaacttct tatatggtct agcagaagga gcaatttctt
    10021 ttagttcact ggtagctgct gttagtattg gtattttctc aaaacatcta aaaatcacta
    10081 atttgcacat ttcctttatt atatgtgcag ttatgttcat accaatggcg gtttcagtat
    10141 atccatttat attaaacaca ggatttccag ttccattttt aatctttata gtatcatcta
    10201 tgctaatatt ttttacaacg actcttatct caatatatgt tataacagtc acacaattaa
    10261 aaactccaaa tgaactctta ggaaaagtaa tgtcaattct ttttgcagga acagcaattg
    10321 caatgcctct tggtcaggtt atgtatggta aactttttga tttattatca gatagagtgt
    10381 acatcattat actaggagtt ggatttatta cactacttat agcatttgta tcaaagataa
    10441 cattgaggaa cgaaaatctt tagatacaaa gaatttagat ataaagattt aattttcact
    10501 aatttctgaa tttaaatgca aaattgcgta tcagtatgtt gacgttttag aaattgattg
    10561 atataatcaa tggaaaataa actttataaa aattcaaaat attcagctag aggagtgata
    10621 aaaatgagtt acatgccaac tttagaacaa gcatgggaaa tattaagaaa atacaataag
    10681 gaagaatttc atataagaca tgctcaaatt gtatctggag ttatgagata ctatgcaaag
    10741 gagtacgacc ctgagagagt agacttttgg gaaatagttg gacttttaca tgatttagat
    10801 tttgaaatgt atcctgatga acattgtgtt aaacaaagag aattaatgac agagcttgat
    10861 ttagataaat caataatcga ttctacaata agtcatggat atggattaac tggttcagat
    10921 gttaaaccag agcaatttat ggagaaagta ttatttgctg tggatgaact tactggttta
    10981 attggtgctg ttgcaataat gcgtccttca aagagcgttt ctgatttaga attaaaatct
    11041 gttaagaaga aatttaaaga taaatcattt gctgctggct gttctcgaga cgttataaga
    11101 agtggtgcag aaatgcttgg atgggaactt gataagctta tccaaacaac tatagaagct
    11161 atgagaagct tgattccaga gatggcaata tagctcctca tacatatacc atagaagtta
    11221 catctttaaa gagcatttct ctttttgatg caacttcttt ttatattttg tagaataaaa
    11281 agtttaatat tttaaactca ctatataatc tataataaat actataaata atataatgta
    11341 taatagtcat aaactttcta accttaaaag tttctaatac tcaataaaca attctaatat
    11401 acaagtggca ttatatgatt taattatata tcttcaattt atatattaaa ataaatatat
    11461 atattaaa
//
LOCUS       DAELSY010000129        11430 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.129, whole genome shotgun sequence.
ACCESSION   DAELSY010000129 DAELSY010000000
VERSION     DAELSY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11430
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.129"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            30..1730
                     /locus_tag="KPG09_002951"
     CDS             30..1730
                     /locus_tag="KPG09_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967540.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            1900..2517
                     /locus_tag="KPG09_002952"
     CDS             1900..2517
                     /locus_tag="KPG09_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967541.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            2878..4071
                     /locus_tag="KPG09_002953"
     CDS             2878..4071
                     /locus_tag="KPG09_002953"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG0967542.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            4089..5381
                     /gene="lysA"
                     /locus_tag="KPG09_002954"
     CDS             4089..5381
                     /gene="lysA"
                     /locus_tag="KPG09_002954"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBG0967543.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            5598..6257
                     /locus_tag="KPG09_002955"
     CDS             5598..6257
                     /locus_tag="KPG09_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967544.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KYSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(6412..7497)
                     /locus_tag="KPG09_002956"
     CDS             complement(6412..7497)
                     /locus_tag="KPG09_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG0967545.1"
                     /translation="MIIILKENIKYYLIVVFIGILFFKFINTPSNFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            7688..8404
                     /locus_tag="KPG09_002957"
     CDS             7688..8404
                     /locus_tag="KPG09_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG0967546.1"
                     /translation="MRINKYIASCGIASRRKSEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            8462..9040
                     /locus_tag="KPG09_002958"
     CDS             8462..9040
                     /locus_tag="KPG09_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG0967547.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            9434..10306
                     /locus_tag="KPG09_002959"
     CDS             9434..10306
                     /locus_tag="KPG09_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0967548.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     repeat_region   11137..11429
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=11137..11163
                     /rpt_unit_seq="gttttatattaactatatggaatgtaa"
BASE COUNT         4674 a         1162 c         2013 g         3581 t
ORIGIN      
        1 taataacatt ttttagagag gagtattaaa tgaaacgata tcttaaaaca tatggagttg
       61 cattaataat tgtaataatt tatgcattta ttaaattacc tgtactaaga ttagatttta
      121 catctttatt ttcagtagga ataattttct ttgttgtagc aggaatacta gatatgatgt
      181 tagatagaaa tgaaagagca tctaaaatgg ctaaatataa tttttcaata gcaatagctt
      241 tgcttgtgta tattgtagta gttcccttta taacttctac acctgtactt catgcaaaat
      301 catatagaga actattggga aaagtaactg aaagtaaatt tactgatgac attagccctg
      361 tcagtgtaaa tgacattcgt cttgtagatg aagatatggc aatgaaatta ggtgacaaaa
      421 agcttggaga agttcctgca atagggagtg tatccaaatt aggaaaattt catatacaaa
      481 atgtagatgg agagttgtac tgggtagcgc ctcttgttca cagagacata attaaatgga
      541 taacatctct aagtggtaca agtggttatg taaaagtatc agctagtaat cctcaagacg
      601 tccaattagt tcaagaaata gatggaaagc caataaagat agtttaccaa ccagaagcct
      661 acttacatca ggatttacaa agacatttat acatacatgg tattgtaaat gttggtatga
      721 cagatttcac tcttgaaata aatgatgaag gaagacctta ttgggttgta agtttatatg
      781 aacataaaat tggctatgga ggagcaaatg caacaggaat agccactgtt gatgcagaaa
      841 ctggaaaaat aaatgtttat gatgttaaaa acacacctaa atgggtggat agaatacaac
      901 cacaaagctt tgtaacagac cagattaaag actggggtgt ttatgttaat ggatttttaa
      961 actctgttat atcagaaaag ggagttttag taccaacaga aggtacatca ttagtttatg
     1021 gcaatgataa caggtcttat tggtatacag gtataacatc ttcaggtgga gatgaatcta
     1081 caattggatt tatgttagtt gattctagaa caaaagaagc tagattatat aaacaaccag
     1141 gagctactga aacggctgca atgaaatcag ctgaaggtaa tgttcaagaa aaaaattatg
     1201 aagcgacgtt ccctgtaatg tataatatat tgggtcagcc aacatatgta tcatcattaa
     1261 aagataaggc tggtcttgtt aagagagttg catttgtatc tgttgaagat tataatgtac
     1321 ttggaatagg tgaagataag aatgaagcat taagaaacta taaggatgca ttagaatcta
     1381 agggtaatga tttaaaactt gataatgatt taaaagatga ggtattagaa ggcacagtga
     1441 caagaataag cccagatgtg cgaggtggta acactaatta ttatgtaact ttagattcaa
     1501 ataaagatat aatatttagg gctacttcaa aagtatctag cgaattgcca ttgactcagg
     1561 ttggtgataa agtcaaaata tcgtattcaa aagaagaatc tggtgtaata gaaatgtcag
     1621 aatttgataa cctaaatatt gcaaactttg aagataaaaa agcaaaagaa aaaacaggaa
     1681 ctgatgaaaa tacagattcc aaagattctg aaaatcaagg taatgtataa tttaaataaa
     1741 aagattgata atagtattta acatagtgat agctaatttt tatagaattt aattctataa
     1801 aaattagcta ttttatttta attctttgta aatttaatgt cataaaatta atataatatt
     1861 agtacaactt tgcaaataat acttcaagga gaatgtacta tgacgaatat ggaaaaagag
     1921 atattacttt taattagcag ggttagatgt gttacagagt ctcaatttag aaaaatttat
     1981 ggagaccaaa aaagatacaa taaaaaaaat tttaaaaaaa ctttgagaaa aatgtgtagt
     2041 gaatatacac taaaaaaata tccttgtgat gtggattatt ataattataa agacctctct
     2101 tatgtatatt atttaaacgg tagtaaactt tttaagggag atgatttaat aaaggctcta
     2161 gtaggatcag aattagttgt tagaatcaat actgcaggtt gtgatgtaaa aagattttac
     2221 cgaaatgtta gtgtagatga tgtgaaatac gatttattta ttgaatatac agaccaattt
     2281 ggtaatataa agcaagtttt agttgatgtt gacttaaata atgaaataga catttctaaa
     2341 tacgaaaatc tttctaaaaa aatagataaa tctacaattc cattttttaa agtaccaaac
     2401 gttttggtag ttacaccaga taatctatcc gaattaaatg atttagagag atatgataat
     2461 gagtatagca taaattttat agatttagga ttaaataaac tatttaattg tatataagct
     2521 aactttagaa aagaaaacta tcgatttgcc acattttgta aaataattgt tgcaatatta
     2581 tatagaatat gataaaacta tattaataaa ttaattaaaa ataatgagcc tagggtagag
     2641 gtagctgtat tcaatagtat tcaataggag ctggtaagca ttgataattg atgaaagggt
     2701 atatagccga agaaattttg ttgacggaca aattttctgg acttatgcag aatatgcata
     2761 aggctgtcac ttttaaagtg gtgagctaca aggttacttg gtctatgaaa tttacacgtc
     2821 caaagtatac ctttgggcgt ttttatttat caaaatttag cttgaggggg agaaagcatg
     2881 agtataattg ttcaaaaata tggtggaagt tcagtagcag atactgaaaa aataaaatca
     2941 atagcagaaa atataataga acgaagaaaa gaaaatccac aaatggtaat agttgtgtct
     3001 gccatgggaa aatccactga tgaatatata actttagcaa aggaattgag taatgaacct
     3061 agtaagaggg aattagatgc tttaatgtct actggtgaaa tgatttcagc atctttatta
     3121 tcaatagcat taaatgcact tggatgtaaa gctataagtt ataatgctta tcagttaaat
     3181 ataaaaacga gtggtcttca tggaaagtct caaattgatg atataaacgt aaacagaatt
     3241 aaaaatagtt tagatgaagg aaatgtcgtg atagtaactg gatttcaggg aatcaacgaa
     3301 gatggtgatg taaccactct tggaagaggt ggttcagata cgtctgctgt agctcttgcg
     3361 gttaaattaa atggaaaatg tgagatatat acagatgttg atggaattta ttttacagac
     3421 ccaagaaaat attcaaaggc tagtaaactt gatgagattg aatatgaaga aatgcttgag
     3481 ttagcgagtt taggagcaca agtaatgcat tcaagaagta tagagcttgc acaaaaatat
     3541 ggtgttgaaa tctatgtcgg acgtacatgt ggtacagaaa aaggaacata tataagaggg
     3601 ggaaaagaca tgaaattaga ggataaagta ataacaggat tagctactag tgatgatgac
     3661 agttctataa caataaaaga ttttaaagct gaaaatatat ctagcttatt tgaagatata
     3721 gcaactatag gtataagtgt agatatgata agccaaactg cacctatttt agataagata
     3781 agtgtatcat ttacagttcc aaaagaagag ttgggagaat gtaaaaagat agtatctaaa
     3841 tatacagatg aagaacatgt agtgattgat aataatatca ctaaattttc tttagtaggt
     3901 cttggtatga aaaatacttc tggagttgct gctaaagtat ttaagatatt caatgaaaat
     3961 ggtataatga taaaacttat aactacatca gagattcgta taacttgtgc tataaattct
     4021 gatgataagc aagtagctat agaaaaaata gcagaagtat ttaatatata aaatttgggg
     4081 aggctattat gaaacttcat ggaactatga ctgtaaataa tgatgaatta tatattggag
     4141 gagtgagttg tttagaactt agtaaaaaat atcaaactcc actttatgta tttgatgaag
     4201 ctttagttag acaaaattgt agagaatata tagaatattt taaggctaga gagggaaaaa
     4261 ataaggttgc ttatgcaggg aaagcttttt tacctcttta catgtgtaat cttataaatg
     4321 aagaacaaat gtgtttagat gtagtttcag gaggcgagtt atatacagct tataaatcaa
     4381 attttcctat ggaaaaagtt ttctttcatg gaaacaataa gactaaagaa gaaataaaca
     4441 tgggattaga tttaggtgta ggtaaatttg tagtggataa tttttatgaa ttagatttgc
     4501 ttgaagcatt atgtgaagat aaaggtaaaa ctcaagagat atactttaga ataactcctg
     4561 gtatagaagc tcatacacat gattatataa aaactggaca gatagattct aaatttggat
     4621 ttgctttagt aaatggagat ttatttagag caatagaaaa attaagtgaa tataaaaata
     4681 ttaaacttgt aggtcttcac gctcatatag gttcacaaat atttgatgta gaaccataca
     4741 tagatacagt ggatataatg atgaacttgg taaaagaaat aaaagaaaaa ttcaacatac
     4801 aactaacaga aattgactta ggtggaggag ttggcgttta ttatagcgaa ggagataaac
     4861 ctaaaactat aaaagagttt tgtgaaacca taatcactaa agcagaaact tcatgtaaag
     4921 agcttggaat agaagtacct acattggtaa tagaaccagg aagatcaata gttgcaaatg
     4981 caggaagtac attgtataca gtaggttcta taaaagatat aaaagatgtt agggtttatg
     5041 taagtgtaga tggaggtatg acagataatg taagaccttc gttgtaccaa gctagttatg
     5101 agtgtagtat tgtgaataaa attaatcatg aggctatgaa tcatgtaaca atagctggaa
     5161 aatgttgtga gagtggagat atattaattg gagatataaa cataatggat attaaaagtg
     5221 gagatatact tataacaaca tcaactggtg catatggata ttcaatgtct tcaaattata
     5281 ataaaatacc aagagcagca gtagtatcag tatttgatgg aaatgataag ataatttgta
     5341 agagacaaag ctatgaggac ttattagctt tagagatata aaatggaaaa gtatataata
     5401 aaaattaaat attaaagttt attatgagca gatttgctag gatatgtatc tctaaattaa
     5461 tttttggttt attgtatcgt tcgtagcata tctgcttatt ttttgtttta ctaacaaatt
     5521 tcttgtaata aatataaaat attttaaaaa ctttataatt atgataaaat ttaaagtata
     5581 tgacaaggag ggatttaatg agaggaagag taattttatt aatatcgata ttatctatat
     5641 ttcttgtagg atgttcattt aataaaaaag atgaaataaa tttagtagag caaggaaaaa
     5701 catatttaga aaaacataac tataaaaaag ctatggaatc attatcctct gcactggaag
     5761 aagatagtac taatgagaat gcaagagcta tgtatatgca agctatgaga atgtcaaata
     5821 tgacggagtt tgaagaatta aaaaattatg agggtgcaat caaggaactt aaacttatag
     5881 agaaaatcaa aaatggttcc tctgttatta aagaagaggc aactaaaaag aaagaagagt
     5941 taactaaatt agaaaaagaa caaaacgaag cagaggaaca aagaaagaaa aaggcaaaag
     6001 atacggctgg agaagatagg tatagagttg aatcagatgc aaggaaatct tcttatagtg
     6061 gaagtagtaa aaataataaa tatagttcta aaaataatga taaaaaatca gatagtaata
     6121 agtctgacaa taataacagt tcttctcaaa atcaagaaaa gccaaataat agtaatccaa
     6181 caccagcacc aactccacaa gatactactg gtggttcaga tagtggtgga aattctcaaa
     6241 actcaaataa tcaataagta taaaatatat gcataaattt aatgaacaaa ttatatatta
     6301 taaaaaatat ctcttagcaa ttttttctta ctaagagata ttttttgaga aataatttaa
     6361 taaataacag ttaaatttta aactaaaaat taaagtcaca gtagagataa gctattgtaa
     6421 acgattatct atatatttat caacataagt ttttacaaca gcagctattg gtactgaaag
     6481 aaaaactcct atgaaaccaa atagagattg tcccaccaaa atagcagata ttacccatat
     6541 agctcctata ccaacttgat tacccataac ttttggttct ataaaatttc catctaattg
     6601 ttgcaatata aatagaaatg caatgaccca tatagttttt tgtggactat ataatatagt
     6661 catggctatt ggagggattg caccaataaa aggaccaaaa tagggaatta tattacctaa
     6721 gaatattatt gaagctaaaa ataaagcatt ttctattctg ataacaaatt gaaatcctaa
     6781 aaatgcaatt ataccaataa taagagagtc taatattcta ccaagtaaat aatcatagaa
     6841 aatatcatga gtagtcctaa aaaattctat aatgttatct gccttgtttc tagcagtaga
     6901 agaatataga aatctcttaa atcccaatgc aattttttct ttgtcatata taatgtatat
     6961 ggatagtata aatcccattg tcatattaaa tagcatagaa gatatactaa gtgtatagat
     7021 taagaagttt ttaggaattt taccaacaaa attacttgct tcttttaata aattatctaa
     7081 actatgttgt atatgaggaa ttaaagtttt tagaaaatct atgtttgaca tatttttttc
     7141 tataaaacta tctatataat cagtatacat aggcatttca tttatcaatc tatttaatgt
     7201 attagctatt gatggaatta atagcctaat ggcaaaggct aaaataaaac ctataaatat
     7261 ataagataaa aaaatggcta aaagtctatg tgctttgaat tttacctcaa aaagcatcac
     7321 aagtggattt aaaagtaaag caagtaaaat agctaataaa aatgggctaa aaaactttaa
     7381 gaatccttca atactagata taaagtttga tggagtatta ataaatttaa aaaataaaat
     7441 tccaataaac acgactatta aataatattt tatattttcc tttaaaataa taatcacttc
     7501 cttaaaatag tgtttagtca gctattacat attaatagta tgtgaaaaga aattacaatt
     7561 atttaggaaa attaaataat ttttttgctt gtttttaatt aaggtctagt atagtataat
     7621 aatgtagcaa aattaacatt gataaaaata ttaaacacac ataatttaag ataaggaagg
     7681 attaagtatg agaattaata agtatatagc ttcttgtgga atagcatcaa gaagaaaatc
     7741 agaagaaata attcttggag gaagaataaa ggtaaatgga agtgtagtaa aagagttatc
     7801 ttttaatatt gatgaagaaa aagacatagt agaatttgat aataaaaaag taaaaccaag
     7861 tgaaaattat atatatatag tattaaataa accagaggga tatataacta cagtaaaaga
     7921 ccaattcaat agacccagtg taatagacat acttaaagat gtaagggaaa gagtatatcc
     7981 aatagggaga ttagattatg aaactagtgg tttattaata cttactaatg atggcgattt
     8041 aacatataag cttacccatc caaagcatga aattgataaa acgtatgtgg caagtgtgaa
     8101 gggaattata agtgatgatg agataagaca attcgaaaca ggactaaaaa tagaagatta
     8161 cacaacggca cctgccaaaa taagggtaac taaagaaaat aaagaaaaaa attattcagt
     8221 gtgtgaaata actatacatg agggtagaaa tagacaagta agaaaaatgt gcaaagctat
     8281 aaatcatcca gttttaaatt taagaaggat ttctgtgggg aaaatagtat taaaagacac
     8341 caaagttgga gaatatagat acctaacaga agatgaaata aaatatttaa aatcaataaa
     8401 ataagataaa aataaattga gaaaaaatga aataaagtta atagtaggga gaaatagtat
     8461 tatggcatat gcaaagtatt taactaaaat tacagatata aataaatttt atttagaaaa
     8521 tattataaat gaaggggaca tagtaattga tgctactatg ggaaatgggt atgatacaag
     8581 gtatctagct gagaaggttg gagaaaaggg ctttgtatat gcttttgata tacaagaaga
     8641 agcaataaag tctacaagaa agaaactaga gaaagaagga tatacagata gagtaaagtt
     8701 gatatttgat gggcatgaaa aaatgaatca atatataaaa gaagaagttt catgtgtttt
     8761 atttaatttg ggttatcttc caagagcgaa acattcaatt ataaccaaac cagatacaac
     8821 tttaaaggct ataaagttaa gtttagaaat gttaaaagaa aatggagtaa taagcattgc
     8881 catatataca gggcatgatg gaggtcaaga agaaaaagat tgcatatata accttgtcaa
     8941 taatttaaat caagatgaat ttaatgtttt agaatctaag tttttaaatc aagtcaataa
     9001 cccaccacaa ttgattttaa ttgaaaagaa aaaagcataa taaatactaa aaaacttgac
     9061 aagattctat taagagttaa tatatatagt gtgacctatt aaaaatactt taaataataa
     9121 gtatacaaca gcttattctc aaaacaaaat atacatctaa atatgcaaaa taagatagat
     9181 aacttttaaa tgagtaaaaa ttgaaaaatt taaaagaaat attggataaa tattaaaaaa
     9241 tgcaaggaaa gatggtataa taaatttaat cgaaaatact atgtgaagta taaggtacag
     9301 aaaagttaga attttatgag tttaagaagc tatgataaaa actaagatga aattgagata
     9361 aaaaataaaa tttttagaga aaaagacagt gttaataaaa caaaatagtt tatataacta
     9421 aaaggagaaa aaaatggaag aaacaaatga aatgaaggac agtaaacatt tgatatcaaa
     9481 tatacaatct caatatacga gattgagtaa gggtcaaaaa cttatagcac aatacatttt
     9541 aaataattat gataaagttg catttatgac tgcatgtaaa ttgggtgaaa ccgtaggagt
     9601 aagtgagtct acagttgtaa gatttgcaaa tgcattaggt tatagtggat atcctaaatt
     9661 acaagctgca cttcaagaac tcataaaaaa taaactaact acagtacaaa gagttgaaat
     9721 ggcacatgat tattccgatg attttgcaat actaaacaaa gttttaaaaa gtgatataga
     9781 taatataagg tctacactag aagaaattga cgagagagcg tttaaagaag cttctaacaa
     9841 acttttaaga gcgagaaaaa tatatatact tggaatgagg agttcttttg tagtagctca
     9901 atatttaggt ttttacttag atataatact tgacaatgtt catataatta gaatggatat
     9961 gggagatgct tttgagcaga ttgtaagaat aaatgaagaa gatgtaatag ttgctattag
    10021 tttccctcgt tattctaaga aatcttatca aattgtcaat tatgctaaag aaaaaggagc
    10081 ccatgtaata tcattaacag atagcttatt tgcgccagta gcatcacttg cagataacac
    10141 tcttctagtt aagagtaata tggcttcatt tgtagattct ctagttccag ctcttagtat
    10201 ttcaaatgcc cttgctatat ctgttggaat gaaggaaaaa gaagatatta aacagcattt
    10261 tgatgattta gagcaaattt ggaaaagata ttctgtgtat gagtaaagtt tgtatattaa
    10321 taagtattat taattattaa aatgttcaaa tatagtgatg tttggataaa atatataaaa
    10381 tagctgactg gcatttaact gacagtcagt ttttttattt aaacaatatt ttgcttaaat
    10441 tttaaggaat atctttgtct atttcttcta aatgagagta tttattcata gttattttca
    10501 tttattatgt ttcttgttga tgatatattt ttttatagaa aagaaaatag ttatggtttt
    10561 gtatctgtta tttttgcact tattcaattt atttttgtga aacctggaaa gataaaactt
    10621 taatagcaga taataacctt gagattgtaa agtaacaaag tgaaaatgta gaaagagaaa
    10681 aagaaatgga aggaagaaaa gtatatagat aaagagatat aacacgtatt ttgatttaac
    10741 tgtataagat gaaaaatttg atgattttga atagtttaaa aaattgttaa tgaaaataaa
    10801 agacttgttg agctaaagtg cgattacata ggaaggatgc aataaaaaga aaaaggaatc
    10861 attggaatag agaatataat aaatacttat aagaatgtag atgcttttag tttgtaaaat
    10921 tatcccattt ttattttata gtatgagttt tatgatataa taaaaatata aaagttttgc
    10981 agtgagcgat ttttgtgata aagtagggtt taatagttga aatataaagc gttgagagtg
    11041 tatgataact gttatcaatt gcactattgc tcgctcactg caagtttagg agaattgtat
    11101 atgtataagt attggaaata cttaatttat tttggggttt tatattaact atatggaatg
    11161 taaagtagga ttggtgtgtt acgaccacac aacgctggac tgttttatat taactatatg
    11221 gaatgtaaag agtgaatttg gcgaactcga tatagagaaa aaaataaagg ttttatatta
    11281 actatatgga atgtaaagga agtttttaat gttgtcgcga ctcttgctgt tgcttgtttt
    11341 atattaacta tatggaatgt aaagctgctt tggcttttgg tcgtataatg ctccttataa
    11401 tagttttata ttaactatat ggaatgtaaa
//
LOCUS       DAELSY010000130        11394 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.130, whole genome shotgun sequence.
ACCESSION   DAELSY010000130 DAELSY010000000
VERSION     DAELSY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11394
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.130"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            293..994
                     /locus_tag="KPG09_002960"
     CDS             293..994
                     /locus_tag="KPG09_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG0967549.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKSALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLLVVFILGHRMRGIVEKMNVKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAVISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIVLS"
     gene            1020..1676
                     /locus_tag="KPG09_002961"
     CDS             1020..1676
                     /locus_tag="KPG09_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967550.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGLRFGQMPMLSVIFQI
                     WGLVATTYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFITDSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDSNKTKE
                     "
     gene            1883..3523
                     /locus_tag="KPG09_002962"
     CDS             1883..3523
                     /locus_tag="KPG09_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG0967551.1"
                     /translation="MFDVKIANGIIIDGTGNSRYKGDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPIKLNLHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDKVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWDGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            3882..5204
                     /locus_tag="KPG09_002963"
     CDS             3882..5204
                     /locus_tag="KPG09_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967552.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMLKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDICCC
                     ITGLEEAYHAMKKENIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIVVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPAVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAI
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            5401..6408
                     /locus_tag="KPG09_002964"
     CDS             5401..6408
                     /locus_tag="KPG09_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG0967553.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDK
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGTMGCPPEILKKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            6446..7843
                     /locus_tag="KPG09_002965"
     CDS             6446..7843
                     /locus_tag="KPG09_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBG0967554.1"
                     /translation="MASTNKNNVTSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKGNIDKINTNKSDINKVNIDTVDTDKLNTNTLTEKNKKVIFKGEDN
                     MSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDYIMKITHNIWE
                     EKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIWSNFGA
                     DGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDNLWIVT
                     QLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEMMLEMT
                     SRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYEVERVT
                     CNQREKNEGYDVSIRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKEEWITF
                     DGMDVLKQMYMGVEE"
     gene            7988..9253
                     /locus_tag="KPG09_002966"
     CDS             7988..9253
                     /locus_tag="KPG09_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0967555.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTDIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            9295..10334
                     /locus_tag="KPG09_002967"
                     /pseudo
     CDS             9295..10334
                     /locus_tag="KPG09_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
     gene            10369..11391
                     /locus_tag="KPG09_002968"
     CDS             10369..11391
                     /locus_tag="KPG09_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0967556.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMVAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
BASE COUNT         4107 a         1297 c         2286 g         3704 t
ORIGIN      
        1 aataacaaag ttttttgatt aaaagtttta tattgaaatg tcaaagtaat aaaatacctt
       61 ataagttaat tacaggttaa aaaaataata tgtgaatttt ctgatattta ctaattatca
      121 ataaagtatt taagtattaa tgtttttagt gagactttaa gacagataat ctcctattaa
      181 agatactata tcttagataa aataaattaa aacaatataa aaacataaca agattacaga
      241 aagaaagctc ataataaaac actaaaaaat ttattttaag gaggaataaa gtatgaagtt
      301 tcaagaaatg attaatagcc caggtctttg gattgtttct agttttttag taattatttc
      361 agttgttcaa gcaatcgtat ttatgaaatc agcacttaaa gaggcttcaa atcttggtat
      421 tgaaagaaaa ggaattaaag cagcaattcg ctcagctagt gttacagcaa ttggaccctc
      481 tctatctcct gttattacat tattatcact ggtagctgtg attggtgctc ctacgacttg
      541 gatgaggctt tgtgatgtag gagctgccag aacagaactt ggagtaatat ctcttacatc
      601 aaatttatct ggagtagaag taggtagtgc tgcatttgga gcagaagctt tttcgtatgc
      661 actatgggga atggctttaa ataacttagg ttggttgctt gttgtattta ttttgggtca
      721 tagaatgcgt ggaattgttg aaaaaatgaa tgtgaaatat aatcctactt gggttaaaaa
      781 actacttgca ggggctacag ttggattatt tgcttacctt ctttcaaatc aaataaaaac
      841 attcgaaata ccaaaactta caccagcagt tatttcagct atagttatgc tagtattgac
      901 gactttattt aaaaagaatc aacgattaca agaattatcc ttaggtattg ccatgttgat
      961 tggtatgttt ggcacacaga ttgtactatc atgattaaaa cagataagag gtgataatta
     1021 tgtatgataa aaagatgata aaaataggga gaatttctat aggtcttgca attattgcaa
     1081 attttattcc tgcaatctat gtaggtcttc gctttggtca gatgccaatg ctttctgtta
     1141 tattccaaat ttggggatta gttgctacaa cttatggagt gagttggatt gttcaaccta
     1201 tagcttatta tccaacacta ggagctccag gttcttatat aggttggctt gcaggttctg
     1261 ttggagatat acgtatgcct gcagcatcaa tggcacagaa aattgcaggt gtagaagcag
     1321 gaactcatga gggtgaagtt gtaggaacaa ttggtacatg ttgttcaatc tttgtatcag
     1381 cttctatgat tactattttt accattgtag gctataaagt aattcctttg ttacctgaat
     1441 ttattactga ttcatttaat tatatacttc cagcattgtt tggggcaatt tacgttgata
     1501 ttgcgcgtaa agatattcga gctggtggat gtacgattgc tgtagcatta gctattatgt
     1561 actttggtga aaaaattggt ataccaggtg gattgttgac attgtttatt gtgttaggtg
     1621 ggatacttat tacaagggtt ttctttgtaa ttgatagcaa taaaacgaaa gaataggatt
     1681 gtttactagg aagaaagact agaattattt gctgagaaaa agaataggat tgtttactag
     1741 gaggaaagac tagaattatt tgctgagaaa aagaatagga ttgtttacta ggaggaaaga
     1801 ctagaattat ttgttgagaa aaaagaatag gattgtttat taagaaatat gaatataaat
     1861 aagtcttgaa aggagaagaa gaatgtttga tgttaaaatt gccaatggaa taatcattga
     1921 tggtacagga aattcaagat ataaaggtga tgtagggatt gtaggagaca aaatcatggc
     1981 tattggagat ctaagtcaaa aagaggctaa agagactata gacgcaactg gaaaatttgt
     2041 atcacctggt tttatagatt tccacactca ttctgattta tctcttgtgt atgacaaata
     2101 cacaagaagc cgtatccata caggtgtaac aacagacgtt attgcaaact gtggtatagg
     2161 agttgctccg ataagagaag agaagaagca ggagttaatt gactatcttg gaacgaggat
     2221 aattggtaca attccaataa aattgaacct tcactggaat acaatgcagg aatactttga
     2281 ttacttaact gaaaattctc cagcagtaaa tgttgtagct tatgtagcac aaggacctgt
     2341 acgtatcaat gaaatgggat tttcaaagga gccagctact ttagagcaac ttaaaaatat
     2401 gaagttggaa gttagaaagg ctatggaagc tggatgtgta gctctttcgt caggattagt
     2461 ttatttacca ggagcatata caaagaaaga agaaatggct gaattatgta aggaattgat
     2521 accatacaat ggatactata tatctcatat tagagatgag ggtgatgaag aaatggaagc
     2581 tcttgatgaa gcaatttata tagctaagac ggcaggagtt cctctacata tttctcattt
     2641 aaaagtcatg ggtcataaga attttggaac tattgataag gtatttaaaa agctagatga
     2701 agctgaggca gatggcttgg aagttacttt tgattgttac ccatatacag ctggaatgac
     2761 atctttgggt gcacttttac ctccttgggc ttttgaaggt ggtgtagaaa acatggtaaa
     2821 acgattagaa gtgcaagaaa atagggacag aattataaaa gagctagagg aagggattcc
     2881 tggatggcaa tgtttctacc agttagcagg tggttgggac ggtgttgtac tagcctctgt
     2941 aatgactgaa gcaaataaat atgtagaggg taaaacttta atggaggtag ctaaaatcaa
     3001 tggtgaaaat ccatttgata cgttctttag gcttttgatt gaagaaaaaa gtaaaattca
     3061 agtagttgtt catacacaag gtcaagagga tacagataag gtcgtttgcc atccaaagtc
     3121 atgtataggt tctgatagta tggacctttc tacagaaggc ttactttctc ttggaaagcc
     3181 tcatccccgt gcatttggta cttttggacg aattttctct tactatgtta gagaaaaagg
     3241 tatgttgact tttgaggaag cagttaaaaa gataacttat ctttcagcaa aacgcttagg
     3301 aatttataaa gaaaggggtc tacttaagga aaattatttt gctgacattg tggtatttga
     3361 cccagataca atagaagata aagcgactta tagtaatcct aaacagtata cagtgggagt
     3421 ggaatatgta ttggtaaatg gtaaaattgc attggcagga ggaaagcaaa ctgatgtatg
     3481 tgctggacgt gtaattaaaa atcctcttag catagcaaaa tagaatacat taagaacttt
     3541 tataaaaata gtctttcttt gaatattaag gaaggctatt tttgttaaat taataaagat
     3601 tactctactt gtgttttcta gagaatttaa ctttaagtag aaaaaacaat atattaattg
     3661 taaaatgtaa ttaatattaa tggataagat ttaaatacaa aaacttaaat acaaaaatgt
     3721 tttaacagat agtcataatt aaaaaactta aagtaacttt ttatataaga tataattgat
     3781 tatgcaaaat tttatgaagc agcaaccttg ttactttaag tcaagggagg ttcagaaagt
     3841 gtcttaagag ataatagtat ctttaggaaa ggagaaacga aatgaaaata ctttatgtag
     3901 gaccaggcat ggtaatcgac aaaatactag cagtaagtaa aaagaatttt cccaatattg
     3961 aagcagattt tcttagatat gatagatata ttgaagtacc tgatttattg gaaaaatata
     4021 gaggtagagt agatgcagtt ctttttacag gtaaatcgcc atttaaattt tttgaaaaaa
     4081 gacaaaaaga ttttgtgttg tctgattata ttcctagaca tgaaacaaca ttatacagag
     4141 tattgcttga aattacatat ttgcttaaat tcgatattag tagacttagt atagatactt
     4201 atgataaagc tatgctgaag agattgtata aagaaattgg agttgaattt aaggatgaac
     4261 atatgttttt tgcagagcaa aagtatttag aagaggacta cataaagttt gtgcttgaat
     4321 ttcataaata taattatcaa aataatgaca tatgttgttg tattacaggt cttgaagaag
     4381 catatcatgc aatgaaaaaa gagaatattc cagtagtttt agccttgcct tctgaggatg
     4441 taatggtaca aagtataaaa aacattcaga tgcgttatat tgcaaagaaa aacaatgaga
     4501 atcagattgt tgttcttgct atcaaactta atatgccttc agaatattca ctgatgaagg
     4561 aagatgagta cgcttatctt tcacaaagga ttaagattct tgataaacta taccatttca
     4621 atagccgtat agatggtgtt ttggtagagc agagtagaag cgaatttatg atttttacaa
     4681 ctaaaaaaat acttgagtta gaaacaaaga agtacagaga tatttatttg ttggatatga
     4741 ttcgtgaagt ttcagttata aacacttata taggaattgg ttatggaaaa acagcaaatg
     4801 aagctaaatt taacgcttat gagagtatta agatggctca aagtcaaaaa aatgatgcag
     4861 catatgtagt ttttgaaaat ggagaaatta tgggaccact aaagccaaat cctgctgtca
     4921 aagagaaaaa tagctttgat gaacgatttt atcaaattgc tacagagaca gggctaagtg
     4981 ctaatactgt atataaagtg ttcaataaaa taataaatag tggaaaaact gaatttacat
     5041 caaaggaatt agctattata tgtggtgtaa gtattagaac tatggacagg attatactta
     5101 gattgtgtga ttctggttat tgtgaggtag tgagtgaaca gcttatgagc aagtatggta
     5161 gacccagtag aatattacgt tttaaacctt ttctgttgtt ttaaatagat ataagttaaa
     5221 aattaaattt ttatgataac attttcttta caatttttta gatatatgtt aatatttaga
     5281 aaaatgcata tgtattcata aaataaactc aaaatagcta aaaaatcatt aaaaaggaaa
     5341 tcgtatgcat aaaatgagaa agttaattta taacttaaat aaatctggga ggagataatt
     5401 atgggaaaat atcaggattc aaaaaaagtt gtaagaaatt attttgaagc attagaaaat
     5461 gctactgggg atgaggttga aaatgtctta aagcaacata tgaaatcaga ttataactgg
     5521 aaaggtgtgt atccatttcg tgaacaagaa ggcactgaaa atgtagcaga tgtattttgg
     5581 aagcctttaa aaaaatcatt gagtaatatg caaagacgac aagatatttt cattgcagga
     5641 acaaatgaga ttgacaatag tgaatgggta atgagtatgg ggcattttat gggacttttt
     5701 gataaggatt ggcttggcat taaacatact cgtaaaatga ttagtttaag atatgcagaa
     5761 tttaactgtg ttcaagatgg taaaattgct aaaactggat tatttgtaga tataattggg
     5821 tttatgattc aagcaggagt taatccattg ccaccacaaa caggttcata ttttgtatat
     5881 ccagggccaa ttgaccataa tggattactt tttgaagatg cagatgaagt tgaaggagta
     5941 aaaactttag cattagtaaa taaaatggta gatgatttat ctgaattaaa tgaaagtggt
     6001 actatgggat gcccaccaga aatacttaaa aaatcttggg ctaaagatat gatttggtat
     6061 ggtccaggtg gaattggtgc ttcttatact atacctagat atcaagagca acaccagcta
     6121 ccatttagaa ataatttaaa aggaaagact ttcaatgggc atgtatgtag atttgcagaa
     6181 ggtaattttg cttgtttctt tggatggcct aatctgacta atgtaccatg tggaggtttt
     6241 ttaggattac caggtggaga ggttaaagca gatatgcaag ttgttgatgt ttattatcgt
     6301 aagggcgata aacttcaaga aaactgggtg ttaattgata ttccttattg gttgaaacaa
     6361 caagggcttg atattcttga gagaactaaa agtattttta atgcttagtt atgcatacgt
     6421 attctaaaac aaagggggat ataaagtggc atctaccaac aaaaataatg taacaagtaa
     6481 agaggaagaa aaagaagttt ctacaagtgg gcttgaatca tgtacggtta aagaaaacaa
     6541 agctaaaatg aatcaaacta attttagtaa tataaatgaa aatcaaacaa atgagaataa
     6601 aacgaacaca tataaaggaa atatagataa aataaacaca aataaatccg acataaataa
     6661 agttaacata gatacagtgg atacagataa attaaataca aatactctta ctgaaaaaaa
     6721 taaaaaagtt atatttaagg gggaagataa tatgtcatct acaaaaattt actatggaga
     6781 ttcattagaa gtaacgcctg ttggagtaat ggattacaat gatttttcaa aacagacaaa
     6841 aagggaacag gaaatacctg gatttgattc taagtataga gattttgtag attatattat
     6901 gaagattaca cataatatct gggaagaaaa aggtataggt gttatttatg atacatatca
     6961 caataatgtt acaatgcatt gtggctcttc aaatttagtt ggtattaaag atgttatttc
     7021 caatactctt caaacattac atgcgtttcc tgatagaaga ttaattggtc aaaatgtaat
     7081 atggtctaac tttggtgctg atggtttttt atcttctcac cgtgttttgt ctacagcaac
     7141 taacttaggt gatagtaatt ttggaccagc aacaggtaga aaaataaact ttagaactgt
     7201 aattgattgt gccacaacta acaatagaat ccacgaagaa tggttggtta gagataattt
     7261 atggattgtt actcaattag gattaaaccc tcaggaagta gcaaagagta tggcaaaggc
     7321 aagtgaatct aaagtgctta gcttacaatc aacttatggt atctgtgaat ctatggatgg
     7381 tcaatttatg ccaactaagt atcaagcaaa agatgattct gttggcgaaa tgatgcttga
     7441 aatgacaagc cgtatttata attataaata tataaatgaa gttaaaaaat actatcatga
     7501 taatgcagta gtacatttta tatgtgataa agatttaaat ggttatgatg agattcaagg
     7561 aatgattgta agtttacttg catcaattcc aaatggaagt tatgaagtgg aaagagtaac
     7621 ttgtaatcaa agagaaaaaa atgaaggtta tgatgtatct ataagatgga gattacgtgg
     7681 aataaatgaa ggtataggat tttttggaca gccttctgga aagcatatag aagtaatggg
     7741 aatcaatcat tatcatatac ttcaaggtaa agtaaaggaa gagtggataa cttttgatgg
     7801 tatggatgta ttaaaacaaa tgtatatggg agtagaagaa taaagctgtt tagagtataa
     7861 ataattttga aatgtatata gggaagtatt gctttataaa atttaagtgg ggaatgtata
     7921 tgaaaaatag atgtttaaac ttttagcaaa aattatgact aaatttaaaa gggggaatta
     7981 taaaaatatg aaaacaacgg ttgaaataga aaatgatatc atattaggaa aagactattc
     8041 aaacctaaaa agaggaattg catttttttc aattgcttta atatactttt tttattgcta
     8101 caattttatg gtgggtactt ttattaaacc aacaatgatt tatgctttag cagatggggg
     8161 atttggcttt tcattaaaac aaacagaaga aatatttgca gttatgtctt ttggtacaat
     8221 tccaggtaca tttatttttg gggtgatttc tacaaaaatt gggaagaaga ggactttaat
     8281 tagcgttgct ttactgattg gattgacaac atttatacca atgttatctc ctacagatat
     8341 tatgttatgg aaaatagcac gtttgtgtac aggagtagtg ttgggaggcg tatttggaac
     8401 agctatgcct ttagttgcag atatgtttcc aagtaaatat cgtggtaagt tagctgctat
     8461 attaactgct ttattttcat tagcaatgat ttttggtggg aaagtatatg gcgttttagg
     8521 tgatgcaaat tggcaaatat tgatgtatac tgcgattata ccacctatag ttggagctat
     8581 cttagcaatc ttctttgtgc cagatgattt ggagtataca aaggaattag ttaaaaaggg
     8641 aaaagaaact ggtgaaaaga ttagctatat aagtatgtat aagggaaaat acttatggat
     8701 tggattggga acgattcttt tatcaggtgc aaattttaca gcatattcag cattttcaaa
     8761 taatgcaact acatacctag tgacaggaat aggaatgtca gcagcagtgg ctgggtctat
     8821 atatagttta caaggaattg gtcaattagt aggatattta ttctggggtt caattgcaga
     8881 taaatttgga agaaaaatac cttttatagg tatggcatta gcagcagtat ttgtttttat
     8941 atatacaaaa ttaggtggag ataatacaac aacattctat atgatatcag tattactagg
     9001 tgttgctgtt ggatattctg gagcatgggg agcatactac acagagttgt ttccaagtaa
     9061 atatcgttcg ttgtcatctg gtatgtcatt taatggagga aggattatat ctacgtttgc
     9121 aatacctgct atagctggta cagcttctgg tactttaggt atgatgacta tcttttacat
     9181 atcaatcgca gtctttgtag caggtgctat tgtatggtta ttcttaccag agacgttaaa
     9241 taataaaaaa taaaatacta ggaatttatt ttgttactta gaaaggggtg gcttatgatt
     9301 tcatcaattg atgtatgtat tataatagga tatttgctat taatgttagt tattggttat
     9361 tattcaggaa aagataataa aaatcaagaa gattatttct tggcaggtag gtcaatgcca
     9421 tggattccaa ttgctttatc agttgcagct actatgatta gtgcaaatgg ctttataggt
     9481 ggtcctggat gggcatatgt agatggaatg tatccagtaa tgtaaaatgt gcaaaaaatt
     9541 cttttgtagt tagtgcattt gttatgaatt tagtttacta ctttatgcta ttggtaggag
     9601 taattttatt tgttttttat gggggaagga catttgaaac ttcaaatgaa ataatgatta
     9661 cttttatttt aaatgaactt ccagtaggag ccattggaat tattattgca ggtgtgtttg
     9721 cagcagcaat gtctagtatc gactctatat taaacagtat gacaactgtt tttacgaagg
     9781 atatttatga gttttatttc aaaaaagaca acaaagaatc atcattaaaa gttacaatga
     9841 ttataactgt agttatagga gttgttatga caggttttat tattatggga tttggaggaa
     9901 gcatcaaatc aattcttgac cttgttggta actatatctc atactttgct ggaccagcta
     9961 caggtgcatt tgtactagca atgtttacat ataaagctaa tgataaagga gtatctattg
    10021 gttttattgt agggttagtt ttaggatttt ttatttctaa taaatataat gtgagttggt
    10081 tgtggaatcc agcaataggt gctacaataa cattgatatt tggatatatt tttagtgtca
    10141 ttttaaagaa aaatcataaa gtagatgata ttaaaaaata cactgcattt ggtatgagaa
    10201 atcaccttat ttctcaagga atggataaag tagatggagt atctatagtg ccattttctt
    10261 taggtaaaca agaactaatt gtattagctt tttttattat ccaatatatt atattatttt
    10321 tgatacaatt ttaagaagaa atactagata tttaaggaga aatactaaat gaatcaaaag
    10381 aaagttcatt ataataaatc ggctacatca aaagatgtcg caagattggc aggagtatct
    10441 caatcatctg tttcaagagc atttggtagt gcaaatggaa agggcgtaaa acctgaagta
    10501 agagaaagaa tttttcgtgt agcaaacgaa atagggtatg ttcctaattt ggtagcacgg
    10561 ggaatgatta gtggaaaaac taatgttata ggtttgatag ttggtgatag tctaggtcca
    10621 ttttacaata gaattatcaa tttatttgta gaaaaaattc aagagatagg taaacagtgt
    10681 ctcgttttta aagttcctag acaacaaagt atagatagta taatttcaaa agtaattcag
    10741 tttcaagtag aagctgtgat tattacagct tcggccatga ataaggtgat ggctgagact
    10801 tgtgaagaaa acaatattcc tgtgattctt tttaatcgtt ttatacctgg tataaaaata
    10861 agtacagtct atgtagaccc aattgaaggt gggggcatgg ttgctgaata tctgcttaag
    10921 aaaggtcata agaatatagg atatattcaa tttacgagag aaacaagtga agaaatggaa
    10981 aagaaaattg gattttattc taagttaaga caaagtggga ttcataacct taaagaagaa
    11041 tctgcaaact atgattatga ttctggatat gaagcaggta agagaatgtt agcattatca
    11101 aatcctccaa cagcaatttt ttgcacaagt gacttgatag ctattggtgt aatggatgct
    11161 gcaaggtttg agtacaagtt gagggtgcca gaggatttat cagtgatagg atatgatgat
    11221 attcaaatgg cttcatggaa gagttataat ttaacgacga ttagacaacc actagatttg
    11281 ttgattgaaa aaacaattaa catacttaag tccttattaa atgaagaaaa tccagagtca
    11341 gtcgttgaaa tgttaaaacc tgaattgatt gaaagaggtt ctacacttta agtg
//
LOCUS       DAELSY010000131        11387 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.131, whole genome shotgun sequence.
ACCESSION   DAELSY010000131 DAELSY010000000
VERSION     DAELSY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.131"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            33..818
                     /locus_tag="KPG09_002969"
     CDS             33..818
                     /locus_tag="KPG09_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG0967557.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            1097..3007
                     /gene="phnW"
                     /locus_tag="KPG09_002970"
     CDS             1097..3007
                     /gene="phnW"
                     /locus_tag="KPG09_002970"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBG0967558.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNLWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            3364..4185
                     /locus_tag="KPG09_002971"
     CDS             3364..4185
                     /locus_tag="KPG09_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967559.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            4226..4993
                     /locus_tag="KPG09_002972"
     CDS             4226..4993
                     /locus_tag="KPG09_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HBG0967560.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGVIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            5001..5621
                     /locus_tag="KPG09_002973"
     CDS             5001..5621
                     /locus_tag="KPG09_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HBG0967561.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(5921..6508)
                     /locus_tag="KPG09_002974"
     CDS             complement(5921..6508)
                     /locus_tag="KPG09_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG0967562.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(6594..7031)
                     /locus_tag="KPG09_002975"
     CDS             complement(6594..7031)
                     /locus_tag="KPG09_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0967563.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            7135..8133
                     /locus_tag="KPG09_002976"
     CDS             7135..8133
                     /locus_tag="KPG09_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBG0967564.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMTGKHVGRTVVDLEKI"
     gene            complement(8253..9179)
                     /locus_tag="KPG09_002977"
     CDS             complement(8253..9179)
                     /locus_tag="KPG09_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG0967565.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTVLYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            9565..11169
                     /locus_tag="KPG09_002978"
     CDS             9565..11169
                     /locus_tag="KPG09_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0967566.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGAANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVLDNNREFIKKYINR
                     FKVGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
BASE COUNT         4491 a         1221 c         1948 g         3727 t
ORIGIN      
        1 agaaaaatat attatattta ggaggaaaat gaatgtcttt aaattcgata gatagaaaaa
       61 aagaaatact taaattatta gattcaaaag ggaaggtaaa tactaaagaa ttagtaaaaa
      121 gattagaagt atcatcagaa acaataagaa gatatttgga agaattagaa aatgaaaata
      181 aattaaaaaa ggtatatggt ggtgctataa aaataaatta tggagtgatt gaaccagaac
      241 atataaagag aaacaatact aacctagata aaaaacaaag tattgctaaa tacgcagcta
      301 agtttgtaaa tgacaatgag tcaattatca tagatgaagg tactaccaat ttgcagattg
      361 tagattatat aatatctgta aacaatttaa aaattattac aaactcttat ccagttctat
      421 caaaattgat ttcttatgag aatcaaggtt tgtttgatgg agaagttata tttattggtg
      481 gaaaagtaaa tttaaaacac caaaggacag catgtccaat gtcaatagac actatgaaaa
      541 acttatacgt agataaagct tttatatcta gtgaaggtat acttgatagt tttggaatta
      601 gtagtataga ccaaaataag gcattgttgt caaaagaata tataaaaaat tcaaaggaaa
      661 gttttgtatt atgtgatagt tctaaaatag gaattgctag tatgtataag atagcagatt
      721 tagaggaatt agattttgtt atttgtgatg tagaacctcc gaatgaatgg aaggataagt
      781 taaatgagat aaatataaca tggatagaag caaaataaac ccttagtttt atataataca
      841 actatgctta atacaactaa attcataaaa tatagtcagt tcatagatta agattagaaa
      901 gtaaatttta gattgtaact taggatttta tggaaatatg atataaaata aaaacaatta
      961 aatacaacta aaaacagaaa tttaatatat atttaaccta aatatgatgt ataattaaca
     1021 tatgaaagtt atactttaaa agtaaaaaca attaaaaaca caaaaattct aaaatgaatc
     1081 tatggaggct ataaatatga aaaagatata tggagaaaaa ataaaagcag tagtattcga
     1141 ttgggctgga acaacagtag attatggatg ttttgcacct cttaatgtat ttatagagat
     1201 atttaaaaga agaggtatag atgtaacaat ggaagaagca agaaaaccta tgggaaaatt
     1261 aaaaatagac catataagag aaatgtgtga aatggataga ataaaaaatc tttggtcaga
     1321 taagtttggt aaagtgccaa ctgaagatga tgtaaatgaa ctttatgctg aatttgagcc
     1381 aatgttattt gagacattag aagagtatac gactccaatt cctcatgttg ttgaaactat
     1441 tgaaaagttg agaaaaaatg gattaaaaat aggttctaca acgggttata caagagagat
     1501 gatgaacata gttgaaccaa atgcagcaaa gaaaggatac tcacccgatt ttcttgtaac
     1561 accatcagaa gtatctcaag gaagaccgta tccttggatg tgctataaaa atgctgaagc
     1621 tttaggggtg tctcctatga gttctatggt aaaagtagga gatacaataa gtgatgtaaa
     1681 agaaggcgta aatgcaggaa tgtggagtgt tgctgtaata aaaggttcaa gtgagttagg
     1741 gcttacacaa gaagaagtag agaacatgga taaggaagaa ttaaaagcta agatgagtat
     1801 agtttcaaag aaatttaaag aggcaggtgc acattttgta atagaaacta tggctgagtt
     1861 agaagatata ctaataaaaa tagagaatga aactattaaa tcagattttg taccagaaaa
     1921 tgattacata ttacttacac caggaccact atctactact aaatcagtaa gagcatctat
     1981 gttaaaagat tggtgtactt gggatgttga atataataac ctagtacaag atgttagaag
     2041 aagattagta tctctagcaa cccaaaatac agataaatac acaagtgtat taatgcaagg
     2101 aagtgggact ttttcagtag aagctataat aggaagtact atttcaaaag atggtaaact
     2161 tcttgtaatt gcaaatggag catatggaaa gagaatgaaa gatatatgta attatcttaa
     2221 catagaattt gttgactgca catttaaaga tatagaagca gtggatttaa atgtagtaga
     2281 aaatttatta aaagaaaaca aggatataac acatatatca atggttcact gtgaaactac
     2341 tacaggtaga ttaaatccta tacaagaggt agggaaactt gcaaaagaat ataacaaaat
     2401 ttatatagtt gatgctatga gtagttttgg gggaattgag attgatgttg aagattttaa
     2461 tatagatttt ttagttagta gttctaacaa atgtatacaa ggagtacctg gatttggttt
     2521 tataatagct aataaagaga aattatctaa atgtaaaggt atagctaaat cattatctct
     2581 tgatgtgtat gcgcaatggg agactatgga gaaaaataat ggtaaatgga gatttacttc
     2641 acctacacat gttgtaagag ctttttatca agctttatta gaattggaag aagaaggttc
     2701 tgttgaaaaa agatatgcta gatataaaga aaatcagttt acaatagctt caagattaaa
     2761 atctttagga tttgatactt tggtgaatga taatgctcaa tcacctgtaa taacaacttt
     2821 cttatatcct aaaaatgcaa agtttgaatt tatggaattt tatacttatt taaaagataa
     2881 tggatttgtt atctatccag gtaaattgac tgatatagat acatttagaa taggtagcat
     2941 aggtgaagtt tatccaactg atatggagag actagctgat gtaatagaaa aatttatcaa
     3001 tagatagaag aagatagtgt cttttaggtt acagaatagt gcggagagcc tactccttta
     3061 ggtgtgaaat gatagcacaa acagtgtata aaaagtattg acagtagaat ttaaagactt
     3121 atattttaga agtttataca atgaatatat aaagattgag taatagggat atattttatt
     3181 taaaccacaa tctaagaaat tagatttcta gtttaaataa agcatttccc tattattttt
     3241 atgcctattt atagaaaata aatgatagtt gctttattaa gtattattat cagacttgac
     3301 aacgtaacaa tgttttgtta tattaggttt aaagataaaa tacggtaaag aaaggatatc
     3361 attatgaatg aagaattaag ttcactaaga tttgatggtg ttggaaaatg taaaggtgga
     3421 atttttgata atgttaatat atcaggaaca ggcaaaatag aaggaagtgt aaaatgtagt
     3481 aaatttgatt cttctggttc agcaaaagtt tttgggacaa cagaatgtaa tgaatttttt
     3541 acatctggtt taagtaaagt agaaggaaac atactggcat caaaagttga gatatcggga
     3601 cttcttagat gtacaggaaa tattaattct gttgaaattg atactagtgg aattattaat
     3661 gtagaaggaa gtataaaatc ggatattatt catggagaag gatatttaaa agcaaatgag
     3721 aatatagatt gtgaaaatat caatttaagt ggaatcttgg agtgtaatgg atttttgaat
     3781 tgtgaagaag ttaatattag tttaaatgga acttctaatt ttaatgaagt tggagcaagt
     3841 agtataaata taaaaaaggg gcatggagat tattgcaaaa aatttcgtat gctagaaaaa
     3901 aagcatatca actttgaaaa agtgtcttca aatttaataa gtttaggtct attatcaatt
     3961 agaaagtata cagaaagtaa attgtttgca aatattatag aagcagatga aataagctta
     4021 gaaaatagtg aagtcaaagt tgtaagagga aaaaatatta aaataggcaa gggatgttat
     4081 gtagaaagtt tggagtacag tgaaagtata gagattgatg aagattcaat agttggtgaa
     4141 ataaagaata taagtgaaaa aataaaatta aagaaggata agtaataaat aggtttaaaa
     4201 ataaaattta aaatgaggta ataatatgga aaaagggtct ataaaaatac ttggagaagg
     4261 ctttataagt agtggtgagt atgaaaatat aaaaatcgtg ggaagttcta aatcaaaagg
     4321 aaatgttaaa agtaacgaag taaaagtcct tggagatgca aaatttgaag gtgacatcaa
     4381 tataaaaaat tttgttgtta acggaaaaac tattataaga ggcaatctta aagcagagcg
     4441 tgtaagagtg aatggtcaac ttaatataga gggagatgca gaaatagatg acttagttat
     4501 aactgctcaa cagaaagtgg atggcaaact taaatgtaat aatgttacaa taagaggaca
     4561 attaagtgta aatgaaaata tgtatgcaca agatataaaa atctatggcg aaatgagaaa
     4621 taaatcaaat atagaatgtg aagatataaa ggtatatgga gtaataaaat gtgatggtct
     4681 tttaaatgga gaaaatatat atgtttattc aggaggaggt tcttattgta aagagatagg
     4741 ggcaactaat ataaaaatag gaagaagcaa agatgctttt tcattaatta aaatcattaa
     4801 tattttttca ggaaagttta aatgtaattt gatagaggga gatactattg aattagaaga
     4861 tgcatcaata aaagatatac gaggcaaaag catatctcta gtagataatt gtgacgttgg
     4921 aaatattgaa tacagtgata atttatcagt ttcagataat tctactgtaa aaaacagtat
     4981 aaaggtgggc tagaaaagtt atggaagaat taatatctaa gaaagattta ttgagcaaaa
     5041 ctaatatatc ttatggtcaa ttatatagat ggaaaagaaa aaacataata ccagaggaat
     5101 ggtttataaa aaaatcagct tttacaggtc aagagacttt ctttccaaga gataaaatac
     5161 tagaaagaat agagttaata ttgtctatga aagaagagac ttctcttgat gatattgcaa
     5221 atatgtttat gaaaaaggat attaataagg aatttgacat tgattttatt ctcagtaaag
     5281 aagttatatg taattatacc aaagaaatat ttcaaaattt atataaaaaa gagcaaagca
     5341 taggtaaaaa agaactgctt atattgagca taatagagaa gtttttagtt aaatctataa
     5401 taacttttga agagttaaaa ttgatagtga atataataga agaaaatttt acatcaatat
     5461 acaatgaaag tggaaagatt tatttgttta gaaagtttgg agttccattc gtagtaggat
     5521 gtctggatta taaaaaagta aagtttgaaa aagatgtagt taagattatt gagattgact
     5581 tgacaaaaga aataaatgat ataagtataa agttatcata gtgtattatc ttatataatg
     5641 tattataata atctacttaa aatacacttt atttaaatat ttagtttaaa gataagattt
     5701 tatgtgagta tgtaaaagtt aaagatagat ttaagtaggt tatagggatt agtaaataat
     5761 aaaatgaacc atgatgaatt actataaatt gtatttaaac tacattttct acttatagta
     5821 atatcaatgg ttcattttat tgttattaag ataatacagt atttataaaa atacttgata
     5881 taaatatagt aaattgttta aatagaatta ttgccttagt ttattcaaat aatgagtttt
     5941 taaaatctaa gaaatatcct tgtggatgaa gacatacagg acaagctttt ggagcttctt
     6001 tacctatatg tatatggcca caatgtgtac atatccattg tatatcttca tttcttctga
     6061 ataaagtacc ttcttctaaa tcagtagcat atctaccaaa tctatcacca tgaacttttt
     6121 ctatttcagc tatattttca aataacttag ctatagattc aaatccttct tcacgtgctg
     6181 tttttccaaa gtttttatat acatcatccc actcttgaag ctcattatgt tgtgcagctt
     6241 taaggtgttg tgctgtcttg tcatataaat ctacagggta tgctcctgat atacttatat
     6301 tttcaccagc aaattctttt aatttagaaa agaattgttt tgcatgtgat ttttcttggt
     6361 cagcagtgta attaaataaa gactctatta tatataatcc ttcttttttt gctacagatg
     6421 cagaaatatt gtatctattt ctagcttgac tctcacctgc aaaagctctt aataaatttt
     6481 ctttagtttt actatctttt aaattcatat tctacctctc tatcataata aatttttaca
     6541 atatttgatt ctttttattt attattccaa taggagatat aattatgctc aatttagtca
     6601 gcatttttaa aattatcatt aatttttttt aaaattctaa gaaattcttg tttttcattt
     6661 tctgtaatat tatggtatgc tgtctcttta acttgtgttg agatgctatt aaaagtatct
     6721 tctataagca gtgccttttt tgtaagcttt atatacgtga tacgtttgtc atttgtacat
     6781 ttttgtcttt ctaaatatcc taggtttata agtttattaa ccaacactgt aacagtggat
     6841 ttatcttttc ctattttagc tgcaatttct ttcattgtaa gtattccatt attttcataa
     6901 agtgcagtta aaatatttcc atgagtaggt attaactcac ctaaaccatt tttactgagt
     6961 ttattttcaa tgaatttaat catactagcc tttgttttac ttatgaaatg tacaatatat
     7021 ttatcttcca taaattattc tccaatcttt aatctattta aattagtttg acataaaact
     7081 atttataaac tataattagt ttaacataaa actaaatgta ttgggaggaa atttatgaat
     7141 aaaaaattta aatcactata tattacagaa gaagaaggaa attttagaag aaatatagtt
     7201 gaaagagaag tagatacatt gccagaaggc gaagttctaa taaaagtaaa atattcttct
     7261 ctaaattaca aggatgcact ttctgcaaat ggtaataagg gtgttacgag aaagtatcct
     7321 catacaccag gaattgatgc atctggtata gtagaatcta gtagttgtga tgattttaaa
     7381 attggagaag aagttttagt aacaggttat gacttgggaa tgaatacttc tggtggtttt
     7441 tcagaataca taagagttcc ttctagttgg gtagttaaac ttcctaaaaa tctttcacta
     7501 aaagaagcaa tggattatgg tacagcaggg tttacagcag ctttatctgt gtataaattt
     7561 atagattctg tagacaagaa tatgggagat gtattagtca ctggtggtac tggaggagtt
     7621 gctgtaattg caactaaaat attgattaaa ttaggttatt cagttgtagt ttcaacagga
     7681 aaattagaag aacaaaaaga agtattacta aacttaggtg ttaaagatgt tatacatagg
     7741 tcagaagtgg atgataactc aggaagacct ctgcttagac caagatgggc tggagtaata
     7801 gatacagtag gaggaaatac tctagcaaca gcaataaaga caactaatta ttgtggagca
     7861 gtaacaactt gtggtaatgc aggaggagtt gattttacaa gttcagtata tccatttatc
     7921 ttaaagggaa ttactttata tggtatagat tctgtggatt gcccaatgaa cttaagatta
     7981 aaaatatggg atttattatc aaatgaatgg aaattagatg atttagatga tatggtaaat
     8041 gtagtatcat tggaagagtt aaatgatagt atagattcga taatgacagg aaaacatgtt
     8101 ggaagaacag tagttgattt agaaaaaata taggcgaata aatttataat taggattata
     8161 ggttttaagc aaaaatgaga tacttgaaat ttacttatga gtatctcatt tttttatgta
     8221 aaaaaattta tgtagatgtt tgtgtaaaaa gattattcat gaacaattaa ttcaaataca
     8281 tctggtcttg aatagtgacc ttctgaatca aaatctagtc tactaagtac tattttttct
     8341 aggtctaaat cagctattag catttcttct ttattgaaaa taggacctgc tacatatttt
     8401 ccaaaagggt caactataca acttcctcct ggacacataa tttctggttc tgcatctagc
     8461 tccttatagt agttaaggtc agttggatac atattttttt caacatattg attgcatcca
     8521 attacaaaac atcttccttc taaagcaata tgcttcatag ttgcttgcca ctcttctctt
     8581 gaatctgcag taggggctat gtaaagtttt acacccttag cataaagaac tgttctagct
     8641 aatggcatat aattttccca acaaatcaac gaacccattt ttccatatgg agtatcaact
     8701 actgtaagag tacttccatc tccttctccc caaatacatc tttctgttcc tgtaggttta
     8761 agttttctgt gtttaccaat tagcttacct tctggagaga aaaataagtt tgtgcaataa
     8821 agggtacaat ttatatcatt accatctctt tctgttatac ccattgatag atatactcct
     8881 gcttcttgtg ctgcttttcc taataaatct gttgtagcac taggaactgg tacagaatta
     8941 tcatagtatc ttttccaatc ttctctacct tccattgttc tacttccaac aacaaatcca
     9001 aatgaaagac ctcttggata tgcaggtatg aatgcttcag ggaaaactac gatatttgcc
     9061 ccttttctac cagcttcttt aattaatcct aatgcttttt caacagtttt ttctaaatcc
     9121 attatgactg gtgacgcctg gacaacagca attcttacat ttttattttc cttgctcata
     9181 aaaaacctcc taaaaaatat ttaaattttt tgcattgaat agattacatg agatgttatt
     9241 tgagaactaa gatacttata ttgtgaataa taatgtaact gtatatttaa aataatacat
     9301 catgatttaa aaaattacaa ttgtacagat ataattgagt ttagatattt aaatttaaca
     9361 acttataata ttttttcaat tagtataaag cctattaatt tgtcttattt acaatgaata
     9421 gtatttactt cttggcaaac aataattaaa aataaaactt ttatatgatt aaactctaaa
     9481 gaaaaagttt ataaagaaga taatataatt aagcagaaaa taatgaaaaa aatttattaa
     9541 agtagacaac gtaaggagaa ttttatgaaa gaaaaaattt attacaatgg aaatatcatc
     9601 acaatggaag attcaatatg tggagatgct attcttataa aagataaaat aataaaaaag
     9661 ataggaacaa aagaagaagt atttgctttg aaaaataaag atacagaaat tatagattta
     9721 caaggtaaga cattgatgcc atcttttata gattcacata gtcacttgat agcttttgct
     9781 actactctaa agctagttcc tttagaagat gcaaccagtt ttaaagacat agtaaaaaaa
     9841 atacaggatt tcaaagagtc gaataatata aaaaaaggag actggataat tggattttca
     9901 tatgataata attttttaga agaaaataag catccagata agagtgtact cgatagtgca
     9961 tcttcagaaa atccaatact aatttcacat gcttctggtc atatgggagc tgcaaataca
    10021 ttggggttaa aacagcttgg tgttaccaat gaaacacgtg acccagaagg tggacatata
    10081 ggtagagtag aaggcagtga agaaccaaat ggatatttag aagaaaatgc attttttaat
    10141 gtagcatcta aaataaaaca accatcatca aatgagattt ttaattcaat tgaaaaagca
    10201 cagaacatat atctaagcta tggtattaca acagctcaag aaggtttaat ggaagaaaat
    10261 caatttaaca ttttaaaagc aatggcaaat caaaataaat taaaaatgga tgttgtagga
    10321 tatgtaaatt tgaaaaaaag taagtctgta ttagataata atagggagtt tataaaaaaa
    10381 tatataaata gatttaaagt aggaggttat aagattttct tagatggttc tccacaaggt
    10441 aagacagcat ggttgagtag accatatgaa aattcagatg atggttattg tggatatcct
    10501 atatacaaag atgaagaagt agaaaagttt atagatattt cactaaaaga aaagatgcaa
    10561 ctcttaactc attgtaatgg agatgcagca gcagaccagt tgatagatgc gtttgagaaa
    10621 gttttaaact taaaagagca aagtagtgaa aataatataa gaccagttat gatacatgct
    10681 caaacagtaa gagcagacca gattgatgat atgaaagtaa taaatatgat accttcatac
    10741 tttgtagctc acacatatta ttggggtgat attcatatca aaaaccttgg agaagataga
    10801 gcgtttaaga ttagcccact taaaacaact atagaaaagg gattaattta cactcttcat
    10861 caggatacac cagtaatagc acctaatatg ctggaaacag tatggtgtgc tgtaaataga
    10921 attacaaaaa aaggtataca aataggagag aatgaaaaga taagcccatt agatgcttta
    10981 aagggtgtta ctataaatgc agcatatcaa tattttgaag aagataaaaa aggttcaatt
    11041 aaagaaggta aattagctaa tttgataata ttagatgaaa acccacttac aatcgaccct
    11101 atgaaaataa aagatattaa agtattgcag actataagag aaggagaagt attgtattct
    11161 ttgaagtaga tgaaagttag atattttata atctataaaa aatagtttta tataataagt
    11221 taggtagaat taaataagat tacagttaaa tcaacaatta tattttttat taaattttaa
    11281 ttattgtatt gaagatttat aagtataatt ttattgattc taccttttta aataactgtc
    11341 gatttattaa agttatctat gtactatatt aaacaattaa gtagtta
//
LOCUS       DAELSY010000132        11356 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.132, whole genome shotgun sequence.
ACCESSION   DAELSY010000132 DAELSY010000000
VERSION     DAELSY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11356
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.132"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            106..2004
                     /locus_tag="KPG09_002979"
     CDS             106..2004
                     /locus_tag="KPG09_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBG0967567.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            complement(2132..3253)
                     /locus_tag="KPG09_002980"
     CDS             complement(2132..3253)
                     /locus_tag="KPG09_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBG0967568.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLEEFKTVIHPEKIADYISELIDNHYS"
     gene            3452..3784
                     /locus_tag="KPG09_002981"
     CDS             3452..3784
                     /locus_tag="KPG09_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG0967569.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            3771..4829
                     /locus_tag="KPG09_002982"
     CDS             3771..4829
                     /locus_tag="KPG09_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBG0967570.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNIKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSVTIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            4822..5892
                     /locus_tag="KPG09_002983"
     CDS             4822..5892
                     /locus_tag="KPG09_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBG0967571.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDISVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSTSSYLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSNMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            5994..6530
                     /locus_tag="KPG09_002984"
     CDS             5994..6530
                     /locus_tag="KPG09_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0967572.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            6647..7354
                     /locus_tag="KPG09_002985"
     CDS             6647..7354
                     /locus_tag="KPG09_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG0967573.1"
                     /translation="MNNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRKLIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVIGYQGEVSKEQN
                     LEKLFMKVVAENRKRGE"
     gene            7356..8072
                     /locus_tag="KPG09_002986"
     CDS             7356..8072
                     /locus_tag="KPG09_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG0967574.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWAGKMIYCIICCSIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLTNMFFT
                     IVGVAVFSTTEMWYVFPYSIPSRLMVVVLHILPNGLPVPEGSELLNNAVILPGVLISV
                     ILFVLFALVTRAWFNNREVK"
     gene            8074..8832
                     /locus_tag="KPG09_002987"
     CDS             8074..8832
                     /locus_tag="KPG09_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG0967575.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            8976..10364
                     /locus_tag="KPG09_002988"
     CDS             8976..10364
                     /locus_tag="KPG09_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBG0967576.1"
                     /translation="MLRSKKSNNKKVEKLRTIFIKYLSLFFVMTISFALLLMLSFSVL
                     LSSGVILPANYSEKQFDKYKEKIISSEKAIEDIIPNLYEYGVYTLDGNLISGTFNKKE
                     SKQVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKLVSEYSSPVLRAYLPKPEMLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNILF
                     SMDKMKLALKNSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAINMSEFIKDVLLQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVSNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(10440..11261)
                     /locus_tag="KPG09_002989"
     CDS             complement(10440..11261)
                     /locus_tag="KPG09_002989"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBG0967577.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
BASE COUNT         4446 a         1238 c         1865 g         3807 t
ORIGIN      
        1 aaacacgaat aatttgcaag aaaaattcat gaatgtaagg aaaatgaaat ttatcttgta
       61 tttaacaaaa taaaatatac ataaaaatta tgggagagtg gaaaaatgga tagtctatta
      121 tacgtaatag agaaagataa acacactaat gaagaattaa gagaaatcct aaaaagcaat
      181 aaaaatataa gatttgtttc cttaatgggg gttgatttag gaggaaatgc aacagatgaa
      241 aagattcctg tagaattatt tttggaggat atagataaat ttttggaatc agctatacag
      301 actgatggtt caagtgttga gctttataat atagctacat taaataatgc gaaagtagat
      361 ttaatgccag ataaaagttg tcattggtat gtagattaca atatggaata tatagacgaa
      421 gaagtaggtc tacctgttgg aacattaaag atacctgcat ttttaataca tgataataaa
      481 aaagtatgtt ctagaggagt acttcaaaaa gcagataagt actttaaaaa gtcaatgtat
      541 gaaatattta gagaatatcc acatgtaata aataacatag gaatagattc agtagatgat
      601 atagaggaaa taatgttaac agcagctaca gaacttgagt tctgggttaa tactcctgag
      661 gataaagctg acttagagaa attatatgta tcacaaagcc taaaagagca atactggaaa
      721 agaactcatg gtattataag aacttgttta gagaaaagtt taattatact tcaaaagcta
      781 ggtgtaaacc ctgaaatggc tcacaaagag gtcggaggaa tacaaagttc tattagtata
      841 gatggaagaa caaaccatgc tatggagcaa ttagaggttt cttggaagtt ttctacacca
      901 ttacaagcag cagacaatga attattagta agagatgtta tagaggatgt atttacatca
      961 catggactag aagttacatt taaagcaaag ccaattcatg gagtagcagg aagtggtgga
     1021 catacacatg taggagtaag tgcgaagtta aaggatggaa gtataaaaaa tctatttgct
     1081 ccaaaagatt taaaagaaga ttatctaagt gaactaggat atggggctct tatgggactt
     1141 ctttacaatt atgaagtatt aaacccaatt gtaacagctt ctaatgatgg atttaataga
     1201 ttagttcctg gatttgaagc tcctgtatgc atagttacat ctttagggca tagttatgag
     1261 attccatcaa gaaataggtc agtgcttgtt ggattaataa gagatatgaa aaatccaaag
     1321 actgtaagat ttgagttaag gtcaccaagc cctctatcta acacgtattt agtaatagca
     1381 ggatgttatc aaacaatgtt ggatggtata aaagcagcag ctaagagtgg tcttagtaca
     1441 aaagaacttg aaaaagagtt atctaaaaat gtaggtgaag aaagtttcta tttagaaaaa
     1501 gatagagcat atagagatga aaatgatgta tttgaacatt atagtttgga agaaagaaat
     1561 gcaagatttg gaataccacc ggctacagtt tatgaaaata tgaaaaactt agaaatatat
     1621 gcttcaaaat taaaatcttt aaaacaaggt gatgtattta ctgatagtat aatagaatca
     1681 tttaagattg gagctataga taagtggcaa aaaaaattaa aaactagaat aatagaggct
     1741 gggattcaaa aaataagaag tattgtcaag atacacacaa aagaaaatat ggatgcgctt
     1801 gatgaggtag tttggaactc aatatcggat ttaaagttca atattatgaa ggatacttta
     1861 actagagaat cactattcac tagagtgaga gaagctatag aaaataagga ttaccagact
     1921 gcttcagatt tacaaataga attaaagaga agtatggaag aaatacaaca actgtatatg
     1981 cagtacaaga aaaatatata ctaatataac aatttgattt tgatatgata aaggcaatca
     2041 agtattatta aacactatga gtaaattctc aagtgtaaaa gcatacttga ttgcctttaa
     2101 ttttatgttt gaaatttttt aatagtacaa tttaactgta atgattatct attaattctg
     2161 atatataatc agcaattttc tctggatgaa taactgtttt aaattcttct aaattttttt
     2221 tcataaagtt tattatagaa tcaaattcat ttgatactaa tttataaaat atgtttttaa
     2281 ggtctaggtc atcactataa atcatgccaa gacccttatc tattataaat ttagcgtttt
     2341 caatttcttg tccaactttt ggcattttca cgagtactgg agtttgtgat tgaatagctt
     2401 caaatatagt tgctcctcca ggcttagtta gcattaggtc acattctctt attagattat
     2461 acatgtcgtt tacaaatcca agtactttaa tattttttag aggtttttta tgagttagat
     2521 tgtcatataa ttttttgttt ttaccagtaa ctattgtaat ttctatagaa ttactatgtt
     2581 cttcaataaa ttcatctatc caatgcataa aatcctcatc tatatcaaat aggcctcttc
     2641 ctccacctag aagtaatagt ctatatttat cacaacatag ttctttactt tcaatttgaa
     2701 atctcttatc aacaggaact ccagtaacct ttatggagtc tggatttatt cctttctgaa
     2761 aaaatctatt ttttatttca ggagaaggaa caaaatacaa atctgtattt tcaaaaaccc
     2821 attccatact atctacaaca tctgttataa ctgtaagtgt aggtatattt atatcaggat
     2881 tttttatctt aaagttattt acacaagcag ccgctagtgg aaatgtagag ataatcaaat
     2941 ctggatttat atcagcaata tactcagaaa gtttaggtgt atataattta tgagataaat
     3001 catgtcttga atcaaacgat ttcttaaagt aataatagta attatataat ccaggagtat
     3061 attttgtatt gttttcataa agttttacca taggtttatt catcatggga atacttgcat
     3121 ttataaagtt ttgtataaca acattatagt tactatattt ttcaataata cattctttga
     3181 ctgcttttgc agcacttata tgtccagcac caaattgagc agttaagatt aatattgttt
     3241 tatcatcttt cattttagcc aatttatcac cactttcata aaatatatct gattagagta
     3301 ttatacattt atagaaggat ttaatcaaca agaatattta aaaataacta ttgtgcagaa
     3361 catagtactg tgttattatg aagatataag aagttttaac atagtacctt gtaaaacaca
     3421 atactgtgta tttaaagaaa ggatggagta tatgaacaca caatttagaa agggtgtttt
     3481 agaaatttgt gtacttgctc tcatatctaa gaaagatatg tatggatatg agatagtaca
     3541 taacatctct aaagtaatag atgtaaatga aggaacaata tatcctattc taagaagact
     3601 tacaaaagat ttgtatttcg aaacatatat actagaatct aatgaaggtc ctgctagaaa
     3661 atactataga ataacttcac taggaaaaga gaatttatcc aatctgatgg aagagtggag
     3721 agaatttgta aaggctgttg agattttaat aagtgaagat gagggaggta atgaacttga
     3781 ataaaactga gtttcttgaa attttaaagg attatctaaa aaaagatttt tctgagagcg
     3841 aagttaatga tatattgaga gattatgaag agtatttcgt agatggtctt atagagggaa
     3901 agagtgatat ggagataata gcatcacttg gttcaccaaa atctatagcc aaagaactta
     3961 tcttgcagac taaagaaaaa aatattgaag aggaaaaaat aataaataca gatgttttga
     4021 atgataagtt tagtaaattt aaatcagata tgaaaaaagg ttactctatc ttaaaagatg
     4081 aagcaaaaga aaagtatagt aaatcaaaag aaaatataga tggaaaaata atgaatacag
     4141 atatattaaa tggtaaattt agtaggttta aatcaaatat aaaaaaggat tactctatct
     4201 tgaaagatga agctaaggaa aagtacaata aatcaaaaga aaatataaat gataaactta
     4261 caccaagtat agaagaaggt gtagggggat tatctagaca agttataaag ggattattag
     4321 tagtgttatc tttatttcta attgttccag catttgcgtt tgtatgtgct attgtatctg
     4381 ttactattgc attagtatgt tcattaattg cttttgttat attaattcca tttgtgatta
     4441 aatttatagc aatggttcca gatgttatga tgttttgtat tttcgcaacc atagcatttg
     4501 tagggcttga gatacttgca tggcaaattt tattatttgc tataaaacta ggtaaacaat
     4561 tactaaataa ttatatacat tggataaaga gaaaaaatat ttatataaag gcaaatgaaa
     4621 agatggaaaa aagaaaaaaa gctactttaa atgatgaaga tttgaataaa gacattaatg
     4681 aagattcata taataatgat tactacaata gagaagaaga aagcataaaa gaaactgatg
     4741 aatataatga tgatagtata aattttgagg cgatatataa ggaccttgaa gatatagaaa
     4801 ataagaaagg agaagataaa catgaataaa aaacttgcta tatttgctgc agttttattg
     4861 gtaataggta taatagggac aacatggtca gggattttag ttatgccaag cctaataaac
     4921 tttggtttag aaaaggaagc tgaatttaaa aaagaaaata aactttatca agaaaaagta
     4981 aatattgaca agttagatat atctgttgac aatataaacg ttacaataaa gaaaagttca
     5041 agtgaagatg tgcgtgtaac aacaagagga aataatgagt tttacaaata caatgtaact
     5101 ttaaaagata agactttagt tgtaaaaggt gaaagaaaat atgaaaataa aattaaaaag
     5161 attaaaaatt tcgaccaatt attaaataca tctataaata gtatgttttc acatgattat
     5221 agagaaatta taatatatgt accaaataat gtagacataa atgcatcaag tacaagttca
     5281 tacttatttg tatatgataa tgtagcttca aatactataa catataaaac ttcttatgga
     5341 ggttttagta gagctataac agagaacaaa gtaaatagat tagaaaacct aaacttaata
     5401 tctaatagca atcttcactt gtcaacaaaa tcaattttag gcgttaaaaa tgtaaacata
     5461 gaatcagagt cattggatat atcttcagag aatgaagatg tatttatcaa taatatagaa
     5521 gagtatatac ctgaaaatgt aaatataaaa gagaaaatag gtagaaattc aaattatgaa
     5581 agtgaatttt acttgtactc taatatgcca attgctaagt tcctggacat agaagttcca
     5641 aattctaagg taagacttga tataccagta aataagtata aatttaattg tgatataaag
     5701 tcaaaagaag caattgaaga atttaataat gatgaagaat atgacaatga ttcatatgat
     5761 tatgaacata gtgaagaaca tagcaacaag tatagaaata cgagggaaat aaaagggctt
     5821 ttgaataaaa atctgtcgaa tttagagaaa gaatatacta taaaaataaa ttcaaatagc
     5881 ttagaattgt aaataattta ctataataat ttataaattg atttaaaagt ataaaaatat
     5941 aatctatact atggtataat aagtatcgaa agagaattag gagagggata cttatgaata
     6001 aaatagcagt aagtttttta ataattgcta ctactttatt gagtacagca tgtatggatt
     6061 atagcatcag tgcagttgag ctagttgatt ctaaagaatc agcagttgta aagaaggatg
     6121 aagatgcaaa agaagaaaca actagtaaaa tgattaattc taaaaaaact actaaaatac
     6181 ctatagagat aatttcaaag gatgagaaaa tagtaaagta cttacagatt gatgaagaaa
     6241 gttcacttaa ggataaactg cgattaatct tagatacatt atcaaatgag tattttaatg
     6301 gtttagacat ggaagtagaa gttaaggaaa aagataatct tgttaaaata aacttgatag
     6361 aaccagataa aaaaagtaga gtttcttgga aagatgatta tttgaatgaa caaaatataa
     6421 tatatactat aaataatatt attaaaaatg tcatccaaga agaagataat agcatttgga
     6481 ttgaagaagt agagatatac tacaatggaa aattaataga attaagataa aaaaagatgt
     6541 gtttttaaca catctttttt ttgtttttta attcttataa aatccttata tttatgattt
     6601 agaatgattc ttgtaaataa gtttgacaaa agaaaggatg aaaattatga ataattatat
     6661 tttgcaggta aaaggagttt ctaaaaaatt taaggagcag atggcagttg ataatatttc
     6721 actagcaatt aaatctaatt ctatatatgg attattagga ccaaatggag ctgggaaatc
     6781 tactttatta aaaatgataa ctgggatttt aaatccaact tcaggaaaaa taatgtttga
     6841 gggacataga tggactagaa aggatttatc aaatattggt tctcttattg agtctcctgc
     6901 tatatatgag aatttaacag cacgagaaaa tttaaaagtc catactactt tacttggtct
     6961 tcctgactct cgaattgaag aagttttaga gactgtcgac ttaagagata cttttaaaaa
     7021 gcgttcaggt caattctctc taggtatgaa gcaacgtctt ggtattgcta ttgcattatt
     7081 aaatcaccca aaacttttaa tcttagacga gcctacaaat ggtcttgacc ctattggtat
     7141 tgaagaactt agaaaattga ttcgttcttt tccaaaacaa ggaattacag ttattttatc
     7201 aagccatatc ttgtctgaag ttgaacaagt cgtagatgaa atcggaataa tttctaatgg
     7261 agtaattggt tatcaaggag aagtatcaaa agaacaaaac ttagaaaaac tttttatgaa
     7321 agttgtagct gaaaatagaa agagagggga gtaatatgga aagatttatt agagtggaat
     7381 ttcaaaagat gaaaggaact tttggaatga agttgatatg gatagttcca gtgttttgta
     7441 tacttttgtg tggactaggt ggagggcaaa atgctgcttt caactggtgg tatacgacat
     7501 ttcttccagg agcattaaca ttaatatctt ctttaatagt attaaaagat gaaaaaagta
     7561 aatatcgtgg tattttacca ttgccaattg aaatgaaaga cgtttgggct ggaaaaatga
     7621 tttactgtat tatttgttgt tctatagcat cgattatttc tgcaataggt gtagtttttg
     7681 ttgggataat ctataaagaa actatttcaa taggtcaaaa tatcattgca actatattaa
     7741 ttattttact atttatgtgg cagattcctc tttgtatgtt tttagcttca aaatttggta
     7801 tatttgttac agttctaaca aatatgttct ttactattgt tggagttgct gttttctcaa
     7861 ctacagaaat gtggtatgta tttccatatt cgattccttc aagattaatg gttgttgtat
     7921 tgcatattct accaaatggg cttcctgtac cagaggggag tgaactttta aataatgctg
     7981 ttattttacc aggagtatta atatctgtta ttttatttgt attatttgct ttagttacaa
     8041 gagcttggtt caataataga gaggtgaagt aatatgagac aactaataag aatattttgt
     8101 tctgatttaa taaaattaaa gagaaatttt ataatactaa tgcacatttg cattgcttta
     8161 atggggatgg gattgttttt atggtattat aaggtttcag gagctgataa tatattaaag
     8221 atagtagcat atttacaagt tatagcaata gcttttcctt tattgtcagg tgttgtgtgt
     8281 tcaatgtttg ttgaacaaga atattatgca gggaattata aacatatgtt aacaacatct
     8341 aatccaaaat atcttactct tataagtaaa tatataatct tagtatgtct tggttttgta
     8401 gcaacattag tgtctgtatt aggttttaaa tttggtatat ctaaggctta ctttacatca
     8461 agcttttata tgatttcaat tctaatttta attgggtgta atttatttga atacatatta
     8521 catttattct taagtttaag gttcggaaaa ggaacatcaa ttggagtagg tatagttgaa
     8581 atgttacttt cagcattatt attaactgga ttgggttctg gcatttggca gtattttcct
     8641 tgtgcatggg gtgttagatt tataactatt tggtcgtctt tttcaagttc taaaacggtt
     8701 gaatatataa aggttgaatc aataaaagca tatcaatcaa caggtttaat gtgtggtttt
     8761 gtaacaattt tagcatttgt aattttatgt atttggtttt ctaaatggga aggtaaaaag
     8821 tcagaagaat aaataaaaat tgactgttta tttacattat atttatatta ttatgttaaa
     8881 atataactta gcaatgaaaa atataaaata atcaaataaa aactggttag gtattaatta
     8941 tgaattaatt aaaatatttg gaggatatgg cacaaatgct tagaagtaaa aaatctaaca
     9001 acaaaaaagt tgaaaagtta agaactattt ttataaaata tttatcttta ttctttgtta
     9061 tgacaatttc atttgcactt cttttaatgt tatcattttc tgtattatta tcatcaggtg
     9121 taatattacc agctaattat tcagaaaaac aatttgataa atataaagaa aagattattt
     9181 caagtgaaaa agcaattgaa gatataattc caaatttata cgagtatggt gtttatactt
     9241 tagatgggaa tttgatctca ggcactttta ataaaaaaga atcaaaacaa gtatggaatt
     9301 taatgagaga tattgaagaa aggcatgcat attcagaaag ttatataaag ttttttaaga
     9361 aagatgaagt ttttataata aaatacaaac tagtatcaga gtatagttct ccagttttaa
     9421 gagcatattt accaaaacca gagatgttag gtatgattat ttttagtata atatttttta
     9481 ttgaaattgt tatattatca aaagtatttg gcaagaaatt taatattgaa atggagcttt
     9541 taaaaaatac tacagaaaaa atagaacaac aggatttaga ttttgttgta gaatctagta
     9601 aaatttatga aattaacaat atcctttttt ctatggataa aatgaagttg gcgttaaaga
     9661 attctttaga aaaacaatgg atgctagaag aaaatagaaa ggaacagata tctgcactgg
     9721 cacatgatat aaaaacacca ttaacaatta tacatggaaa tactgactta ttaattgaaa
     9781 taaatgaaaa tcctgagtta ttagaataca tggaatatat ctcaaagggt gtcactcaaa
     9841 tagaaaaata tattaatact ttaatagata tatcaaaaac agagaatggc tatatattaa
     9901 ataaagaagc aattaatatg agtgagttta taaaagatgt cttactgcaa atagaagcat
     9961 tagctagtac taaaaactta gatgtggagt tttataaaca agataatttg cctaaaaata
    10021 taactataga tagagaactg ttattcagag ctattatgaa tataatttca aatgctatag
    10081 attattcacc aagccagagt aaactataca taagtgtatc agtaagtaat aaatacttaa
    10141 aatttgtagt tacagattgt ggaagtggtt tttctaaagc agatttgtca aaagctacag
    10201 aacagtttta tacaggagat ttgagtagaa attctaaatc tcattacgga atgggacttt
    10261 atattgtaaa taatattgta caaaaacata atggaatttt atatattgaa aattctgtga
    10321 aaactggtgg tgctatggta acaattgaaa taccaatagt ttagtaaata atatccattt
    10381 tgtaaatacc taaattttat gatttatagg tatttataaa atggatagtt tttttgattt
    10441 tatatattag ttaaatctga taaaaagttt tcaaacataa ctccatcatt tctatcagta
    10501 tcaaattgag atgtgtaatt tatagtttta tatataaaat ctgttgcagt attaaccgct
    10561 actcccaaat catgtttatt tactaacata ccacaaagta tagatgtgaa tatatctcca
    10621 gttccactgt aagaacaatt attgtatttg actgatgtaa agaatacatg gtcattatct
    10681 ctatcataag ccaaatttaa tatattatca tcttctaata taccagtaat aacgactttg
    10741 ctaggtccca aatcagatac agattttgca atatatataa gctcatctct attataatcg
    10801 cttttagtat aatcatttcc agttaagaaa caagcttctg ttaaattagg agtagttaaa
    10861 tctgaatgtt taactaattc tttaatttcc tgtctcattt cttctgtgaa tattggatac
    10921 ataactccat tatcacccat cacagggtca actacgataa aagcattggg ataatcattt
    10981 ataaaattag ccactatctc aatttgatgc tttgaaccca aaaaaccact atatatacta
    11041 tcaaaattga cttttaaatt tttccaaaca tttgaatatt ttaccatttc atcagtaaag
    11101 tctaagaaag taaattcagg ataaccagtt tgacttgaaa gtatggccgt tggaaatgga
    11161 caacattgaa cctttaatgc tgaaagtata ggtatagcaa ccgataatga gcattttcct
    11221 attccagata agtcattaat tgctgcgact tttttttgca taaaaaaccc tccattagta
    11281 tgtattaatt ataatgatac caaaatattt atatacatat atattactat aaaaaaaaga
    11341 aaaaagtaga ttataa
//
LOCUS       DAELSY010000133        11268 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.133, whole genome shotgun sequence.
ACCESSION   DAELSY010000133 DAELSY010000000
VERSION     DAELSY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11268
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.133"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            391..1251
                     /locus_tag="KPG09_002990"
     CDS             391..1251
                     /locus_tag="KPG09_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0967578.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1241..3112
                     /locus_tag="KPG09_002991"
     CDS             1241..3112
                     /locus_tag="KPG09_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0967579.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRLTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEASNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3132..4586
                     /locus_tag="KPG09_002992"
     CDS             3132..4586
                     /locus_tag="KPG09_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG0967580.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLMDLYLKYSETVI
                     RRFDDKVKYWITFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            5610..6818
                     /locus_tag="KPG09_002993"
     CDS             5610..6818
                     /locus_tag="KPG09_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967581.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNVLPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFRLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            6849..8228
                     /locus_tag="KPG09_002994"
     CDS             6849..8228
                     /locus_tag="KPG09_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0967582.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELSAY
                     GHGCGHNLLCTAHVGAVVGLKREMLENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            8334..9524
                     /locus_tag="KPG09_002995"
     CDS             8334..9524
                     /locus_tag="KPG09_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0967583.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEESTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEIIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(9652..10716)
                     /locus_tag="KPG09_002996"
     CDS             complement(9652..10716)
                     /locus_tag="KPG09_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967584.1"
                     /translation="MFLKLIFKYNTLDFSRAYFDLTDWADNPKDISYYLSNGTFGDLM
                     DSIDENASKMSLSDIAKLEKKIAKKHINLWNEGKIDFSTTRFSSIVPMLQNAGVNFHF
                     IYPDIDLLKNTFNNLIKDIHLHRMKGNQPSVIYITVKDTNYDDSTLDQVYKILNSIKK
                     DKLTDFIIEKNSNSFKIFTKCETIKSLTKNFTACYIKESIENSTNIKVCVGYGIGNDI
                     KQAMSSAISANKESKINPLNYSFLVNENYETIGPLLCDKLLTVSNKVTPHINTIAKSS
                     KLSTLTIQKIISVTEILQRDEVTPKELSSCLNVTVRTANRFLSSLLKSGNATILYEKQ
                     NSTKGRPERVYKLFISENKY"
     gene            complement(10864..>11268)
                     /locus_tag="KPG09_002997"
     CDS             complement(10864..>11268)
                     /locus_tag="KPG09_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0967585.1"
                     /translation="RLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIKNRRL
                     SRLISDVSWSEFIRQLEYKANWHGRKIVKVGKFFASSQICNKCGYKNEEVKDLNIREW
                     ICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         4155 a         1297 c         2011 g         3805 t
ORIGIN      
        1 agaatttatt tattaacata cagttcatta tatttaaaat cattactatg ataattcata
       61 atagtaaaat agataatggt tcagacaata ttgtaaatat tagtgacagt aaaatagtat
      121 caatttagta tataaacttt gccagataat cgcataacag agcttgtagt tatagaataa
      181 aaaaatatct aggaaaccgt tgacagattt atatggatag tataacatat agaatatgaa
      241 attaaacaaa aaagaatttt aattatggcg acgtagtcat aattttgagt atgttactga
      301 ttagcaggca gaactcaagt atatatgtga aaaatagagg gatactctat acatatatgc
      361 ttgagttctt tttattgaaa ggaggaatat atgaaaataa ttcagatttt aaataataat
      421 gtagccttag taaaaagagg taaaaatgaa attattgtat ttgttaaagg aattggcttt
      481 aaaaaaagag tgggacaagt aataaatgaa aatgaaatag agagaacata tatactagat
      541 tcatacgata tgctagaaca ctttagttat ctactacaaa actcagactc caataatata
      601 gttcttatta atgaaataat tgaatatggt gagaaagctt taaatataaa gtcaagtgat
      661 tatctaagct taaccttact agaccattta gagtttttac ttaaaagatg tgaaaagaat
      721 caatttataa aaagtccttt ggtatggaat gttaaaagat tttatccaaa gcattttgaa
      781 attggaatgc atgcactaaa attaattggt gatagtaagg gaattaatct tcctgatgat
      841 gaggcagtat caatagcact tcattttgta aatatggagg taaataaaga gtctcatgat
      901 agtacaattg tagagttgag aactttagca gatatagttt ctataataaa atatcatttt
      961 aatgtagagc tagatgaaac ttcaacaaat tatatgagat ttactactca tctacagtac
     1021 tttatccaaa gaattatgtc agatgaagct tcaatagatg aagataattc tatagattta
     1081 tataaacaag taagtcaact ttatccaaag tcatttggaa gtgttcaaaa gataagaaaa
     1141 tatataaaat cacaatttaa tgtcgatata tctataagtg aagaaactta tttaatgttg
     1201 catataaata gggtgactca aagactggag gcaaaatcag atgaaatata aagatttaaa
     1261 tgaaaatata ataaaactta tagggggaaa agaaaatata aagtcggtag cgcattgtgt
     1321 tactcgttta agacttactt taaatgatag aagtatcgct caaacagaag aaattaaaaa
     1381 attaaaagga gtaattgatg tagtttctaa tgatgttgct tatcaaatta taataggtac
     1441 tcatgttgta gatgtatata acgagtttat gtctatgata ggtttaagtt caagtgaatc
     1501 aagtataaaa agtgaaagaa aaaagggaat tagaggtatc aaaggtatat ttaattccat
     1561 ctttgtggta gtctcagaga ctatgacttc agtaattgaa gtactacttg cagctggtat
     1621 actcgcaagt atattagcac ttttaaatat gtctggcata cttcagagtg atagccctac
     1681 atatcaaata ttagatacat taagaagtgc tacatttcac ttcttaccag tattaattgc
     1741 aatagcatca gcaaaaagac ttaatgtaaa tccctatata gcagtagtgt tagcagttac
     1801 gttattatcg tcatcaatag atggtgtaga aggattaagt ttatttggta taaatttaca
     1861 ggctacaact tatgcaaaca gttttattcc aatactttta ggagtatggg caatgggaat
     1921 tataattgaa ggtttgaaaa aaatattacc taaagcacta cactattttt tagttccagt
     1981 gttatcttta ataataaccc tatcagttac attaactgta tttggaccta ttggtatgtg
     2041 gattggtgat ggattaaact atgcatgtat gtttttaatt gatacattgg gtaattggag
     2101 tgttgttgca ctgtatgcag ctttacaacc actcttaata gtttttggtg cagcaaactt
     2161 tactatgcct atatctttaa attctgtgac aacattgggg tatgacccaa tttttatggg
     2221 cgctgcaaca atatctgata ttgcagtttg tggtgcaatg tttggatatt tcttaaaaac
     2281 taggaataaa gaacaaaaac aattatttgg gacagtaagt tttagtgcac ttatgggagt
     2341 tacagagcct tcagtgtttg gtgtgtttat gaaatataga agaccatttg tagcagtaat
     2401 gataggtgga ggtataggtg gtttaatagc aggtttagct caagttaaaa catatggaat
     2461 ggtttgggga ttagctgcat taccaacata tcttgttgga ggagaggcat ctaactttac
     2521 atttatgata ataagtgtag tagtatcatt tgtagcagca acagcagctt cctatttact
     2581 aggaatacct tcagatgaaa aagaagaagt taagggtgat aatgaaaaag aattagaact
     2641 aataatcaac gagaacaatt caagaactat tagtattgga aatgttgcaa aaggtcaggt
     2701 aattgcactt ggggaagtta atgacaaagc attttcctct ggagcattag gtaaaggggt
     2761 tggagttata cctagtgaat caaaatcaac tgttatatct cctgtagatg gaactataac
     2821 tatagtattt ccaactaaac atgcatatgg tatagtgact gatgaaggat tagaaatatt
     2881 aattcatatt ggtatagata ctgtaaactt agatggaaaa ttctttgaaa gtcttgtaac
     2941 acaaaatcaa agggttaaaa aaggagatgc tttagcagta tttgaatctg ataaaattat
     3001 agaggaaggc tttgattcta ctataataac tgtagttaca aatactagcg attatttaga
     3061 tgtaatctca tcaaatgatg aatcagaaga attattaact gtaatagtat aaattttagg
     3121 agggaaaatt catgaatatt aaagatacat ttttctgggg aggaagtatt gcagcccatc
     3181 aatgtgaagg ttcatgggat agtgataaca aaggacctgc aattatggat tttgtaacta
     3241 agggctctta tgaaacgcca agagtaatta ctgataaaat agaagaaaaa ttggattatc
     3301 catctcataa tggaatagat ttttataata gatataaaga agatatagct ttatttaaag
     3361 aaatgggatt tagtgcatta agaatatcta tagattggtc aagaattttt ccaaatggag
     3421 atgatgaaaa tcctaatgaa ttagggatta aatactatga aggtctgata gatacattaa
     3481 tagaaaataa tatagaacct attgtaacac tatatcattt tgaattacct atgaatttag
     3541 tacataaata tggttcatgg aataacagaa aacttatgga tttgtattta aagtacagtg
     3601 agactgtaat aagaaggttt gatgataaag taaagtactg gattacattt aatgagatga
     3661 accatattga ccctcaaaca gagcactcgg atatttttac ttatatgata gcaggtatta
     3721 agtatagtga aatagaaaat aaggttcaaa cattggcaaa tgtagggtat aatatgactt
     3781 tggcgggtgt aaaggttgca aagttggcta gagaaatcaa tcccaacaat aaagttggat
     3841 gtgtatttgg actaaatcca atttatccat acaattgtaa tcctagtaat gtgttaaatg
     3901 catttttaga taatgatcgt gattattatc aaattgatgc aatgtgcaat gggaagtttc
     3961 cccaatataa attaaaagaa tatgaaaaaa ataatataac tattgaagtg actgatgaag
     4021 acaaaaaagc atttaaagaa ggtaagttag attttatagg tcttaattat tatttttcaa
     4081 gtgtatctac tccaaaagaa aatttatcag gtgataaatc tttatttggt gggattcaaa
     4141 atccatattt ggaacaaagt aagtggggat gggcaataga ccctataggt ttaagattta
     4201 ccttgaacta tatttacaga aagtatcaat tacctatctt aattacagaa aatggtttag
     4261 gggcatatga taaaatcgaa gaagatggaa ctataaatga tgattataga attgaatatt
     4321 taagtaagca tctatctcaa atgaagtctg caattgaaga ggataatgtt gaatgttttg
     4381 gatatttgat gtggggacca attgatttag tcagtgcaac tacaggtcag atgagtaaga
     4441 gatatggatt tatatatgtt gacttagatg atgaaggcaa aggaagtttt aagagatata
     4501 agaaaaaatc atttgattgg tacaaagaag taataaaatc taatggagaa gttttagatt
     4561 tttctacgga tatagtagta gggtagtaat aagataaata gaagttagga actaatgtta
     4621 attagtgtct agtaaagcat ttaaaataga gtatagggtc tttaaagtaa agtgtgtatt
     4681 cttattaaaa gtaatggcaa ctaaaattac tataaaaagg agaatacaca ttttttatgt
     4741 ttatacttaa gtaaatattt agacatattc atagtataat ttactcatta tataaaaata
     4801 gtatgattta ctcactatat aactttataa attttctaga aaaatgtcag aatatattga
     4861 caaaaaaaag atttgaaatt ataatctata ttgagaatag tttgacaata taaaaacaag
     4921 tatttttaca aatagtgaaa ttatttttat tgtcaattta agaataattt aggactacta
     4981 taaagatgaa ggtatagatg tcattatatg ataaatcata cttgtataga aagaatttta
     5041 aagtatgctt atcattgatt taatataata aataattttt gtttctagta atagttcata
     5101 ggaataagat atctaaattc gcttagaaat agtctaattt acaaaggagg ggatgcacta
     5161 gtagataaaa tttttatata aagaagactt aaagtggagt atattctgat aatattggtt
     5221 taatggttat attattattt cgttttagat attttaagaa taatttaaga cgtttgtaaa
     5281 aatgtattct atatctaata tttaaataaa tataagtctt ccaacatgta tactaagata
     5341 gaaagtagtt gaagtacaat tctaacttgt aaaattatgt agaaagtaaa aatcaaaaag
     5401 aaatctagta agttgataat tatagttaat atttagagag ttaactctag ttatatttat
     5461 ttagatattt taggaataat ttaagacgta tataatatca tcaaaaaata tcaagctata
     5521 ccatatgtag ttaagaataa ctgcataata tacattttat aattattgtt ttattgataa
     5581 aaaattatta tatttaggag gaaacaacaa tggaaaattt ttggaatcca cttacttgct
     5641 ttactgtatg tgtaatgatt tatgctttta gtgagttttt atctaagaaa acaaaaggtg
     5701 caatttcaac attgttattt gtatgtatta tatttttact aggattttgg acaaatgttt
     5761 taccaaaaga cattacagga gcatcaggga tattagctgc aatgagcaac tttggtatag
     5821 ctttattaat aactaactta ggaacactta taaatcttga agacttatgt catgaatgga
     5881 aaacggttgt aattgcatta gtaggtattg taggaatagg ggttggctgt ttaacagtgg
     5941 gtagtatgtt atttggtaga gagtatgctt taatagcagc acctccaata gcaggttcta
     6001 ctgttgccgg tattatcgta acaactgttg cagaagctgc aaatagacct gaacttgctg
     6061 catttgtaat tctagtatta tcatttcaaa agttctttgg cattccgata actacatatt
     6121 gtataaataa agaattaaaa tttaaaagag ataacgggga ttttttgaaa gatgagtcaa
     6181 aaagtaattt tagacttcca agtatgagaa tttttaaaga agatttaggt aaaaaatcat
     6241 ctactctagt gtatcttgca aagctttcat ttgttgctat gattggtaat tttgtaggat
     6301 tggctacttt aataccagga tttacccctg caaactatta tttaaatcct aatatagcat
     6361 gtttgttagt agggttgatt tttacacgaa taggtttttt agaaaaagga attttagata
     6421 aatctcaatc taatggaatt ataatgtttg gttgtatgtt gatgttacct ggaggacttg
     6481 ctaaggtaac tccaacagca ttgtttggga tgattgttcc aactatagga atacttgttg
     6541 taggttctgt atttataata ttatttactg caattgtagg aaaactattg ggatattctc
     6601 caagaatgtc agctgcaatt ggtgtaactt gtatgctagc ttatcctgca acacagataa
     6661 tttccaacga aggtattgat gctatggaag gaactgaaga agaaaaacaa agggcaacag
     6721 attatttatt accaaagatg attataggag gatttgttac tgtaaccatt gcatcagtgg
     6781 catttgctag tataataggt ccaattatat ttggttgata gtaaatatac ttataggagg
     6841 aataagaaat gataggaagt atatctataa atgagataga taaaaaaaga ttcaaacttg
     6901 atgatttaag taagaaaata tgggaaaacc cagaaaaagc atttaaagaa tttaaagcat
     6961 gtgaaaatac agcaaacttt ctaagaagtg aaggatttga tgtggaagta ggtgtaggag
     7021 gtttagccac ggctattaga gctagttttg gttcaggtaa accagttata ggttttatgg
     7081 gagaatttga tgcattacct ggtttaaatc aaaaagtttc aacaaagcaa gaagcatttg
     7141 aattgagtgc atatggtcat ggatgtgggc ataatttatt atgtacagca catgtagggg
     7201 cagtagtagg acttaaaaga gagatgctag aaaataactt aagtgggact atagtatttt
     7261 atgcatgtcc aggagaagaa caattaacag gtaaaggatt tatggctaga ggtggagcat
     7321 ttgaagggtt agacttagct ataaattttc atccaaataa aattagtgaa gctacaattg
     7381 gtacttcaac agctgttaac tctgtaaaat ttcactttaa gggaaagact gcacatgcag
     7441 gctcagaccc tcaaaatgga agaagtgcac tagatgctgt ggaacttact aatgtgggag
     7501 ctaattattt gagagaacat gttacttctg atgtaaggat tcattataca attacagatg
     7561 gaggagtagc acccaacata gttcctgata aagcatcagt atggtattat acaagagctt
     7621 taagtagaga agctgtagaa agtacatatg aaagacttgt aaaggtagca aaaggagctg
     7681 caatgatgac tgaaactgaa gttgaagtag agtttttagg aggttgttat aatactttaa
     7741 ataatcatgt tttagcaaat cttgtatcag aatgtatgaa tgagattaaa caagagcctt
     7801 ggactcgaga ggaattggat tttgcagctg aattagatag acaaagccca caacaatata
     7861 aggctatgat aagtaaatat aatttgccag aaggaactca tctatattat ggtggaggaa
     7921 atgttacaaa ctttaatagt tatggttcta ctgatattgg agatgtaatg catatagttc
     7981 cgacagcata cttttttaca gggtgtacga atctaggtgc accaggtcat agctggcagt
     8041 ttacatcttg tgcaggaagt tctataggag aaaaaggtat gatatatgca tcaaaaatca
     8101 tggctatgtt tggagccaaa atattaaata atccagagat agctaaaaag gcaaaagaag
     8161 agtttgataa atctatgaat ggtaaaacat ataaatgtcc tattccagat gatattccaa
     8221 ctccttaaaa agacatgaag tttaatatat aaaattataa tttttatatc agcatcattt
     8281 cattactaaa agaaagtgag attttgctga ctatctgatt aggaggaagg aatatgaaaa
     8341 atttttttaa agaagcgcag tcaatagaag gggaaattat tagatggaga agatatttac
     8401 atgaaaatcc cgaaattggt tgcaatctac cgaaaacagt aacctttgtt accagtactt
     8461 tagaaaaaat gggatattct ccgaaattga cgtctaataa tggaatagta gctgttttag
     8521 acagtgaaaa tggtggaaag accttattat taagggcaga tatggatgca cttcctatta
     8581 tggaggagac ggatttacct tttaaaagta agaataaaga tgcatctcat agttgtggac
     8641 acgataatca cacagcaatg ctcttaggag cagctaaaat acttatggat aataagcact
     8701 tacttaaagg tagagtaaag tttgtatttg aaccagatga agagagtacc agaggtgcaa
     8761 aatcaatgat tgatgatggt atattagaaa atcctcaggt cgatgcagct atggcatttc
     8821 acagtatggt gggtaaattt cttaacacag gtcaagtagc ttattcacgt ggtccagcta
     8881 tggcatctgc ggatatattt gaaataacag tagaaggaaa agcaactcat ggagcaagcc
     8941 ctgaatttgg agtggaccca ataaacataa tgtcgcatat acaaatagca ttaaacacta
     9001 tactttcaag agaaaaacct cagaaagagc ctgtagtttt aactattggt atgtttaatg
     9061 caggaaacgt accaaatgct ataccaaata aagcatctat gtcaggaact ttaagaacat
     9121 ttgaccaatt tgttagagaa aaattaaaaa atagaattat tgaaatatct acaggaatag
     9181 ctaaatcatt tggaggaaat gctaaagtgg aatttaaaat ggggactccc gcagttataa
     9241 atgatttgga tgtaggagat gagattgtat cttatgttaa agaaattata ggaaatgaga
     9301 atgtaataga ctttccatct gcaatgggtt cagaagattt ttcagagatt ttactaaggg
     9361 ttccaggagt atttttatgg attggaatgg gaagtattag tcagggatat aattttggta
     9421 tgcataatcc taaagcaatt tttaatgaag agggattagt ttatggttca gctttatttg
     9481 ctcattgtgc agtaaaatgg ttagaaaata attttagtag ataataacat aaaatattat
     9541 taaatatcat aaaaggtata tcttaaaaag atgcaaagat atacctttta gaaagtcata
     9601 tataatttta cttaatatta gatgataaaa tatttttatg cattttggat tttaatattt
     9661 attttctgat ataaataatt tgtataccct ttcaggtcta ccttttgttg agttttgttt
     9721 ttcatataat atagtagcgt ttccagactt taagagacta cttagaaatc tattagcagt
     9781 tctaactgtt acatttaaac atgaagataa ttccttagga gtaacttcat ctctttgtag
     9841 aatttcagta acagatataa tcttttgaat agtaagagta gacaatttag aacttttggc
     9901 aattgtattt atatgtggag ttactttatt tgaaacagta agtaacttat cacacagtaa
     9961 aggtcctatt gtctcataat tttcgttgac taagaaactg taatttaaag gatttatttt
    10021 agattcctta ttagcactta tggcactaga catagcttgc tttatatcat taccaattcc
    10081 atatccaaca catactttaa tatttgtact attttcaata gattctttta tataacatgc
    10141 agtaaaattt ttagttaatg atttaatcgt ttcacattta gtgaaaattt taaaagaatt
    10201 actatttttt tcaattataa aatcggttaa tttatctttt ttaatactat ttaatatttt
    10261 ataaacttgg tctagagtag agtcatcata atttgtatct ttaactgtta tatatattac
    10321 acttggttga tttcccttca ttctatgtaa atgaatatct tttattaagt tattaaaagt
    10381 attttttaat aaatctatat cagggtatat gaaatgaaag tttactcctg cattttgtaa
    10441 cataggaaca atactactaa aacgagtagt agaaaaatcg attttccctt cattccatag
    10501 attaatatgt ttttttgcaa tctttttttc aagttttgct atgtcagata aactcatttt
    10561 agatgcattt tcatcaatac tatccattaa gtctccaaat gtgccatttg ataaataata
    10621 tgatatatct tttggattat ctgcccagtc tgttaagtca aagtatgctc tagaaaaatc
    10681 taatgtatta tatttaaaga taagctttag aaacatatca tagtaacttt gtaagtcagt
    10741 accaaactga acctcccatg actaaagtca cagggttcct gcttcataga attttgcata
    10801 ctaaaatatg tcttactaat tcttcacagg ccatccccgt agttcctacg gttcttacat
    10861 aagttattta ttttgaattg ttattagtct taatccttct tttaatatat ttatacttgc
    10921 atttatatct ctatcgtgag tttcattaca actaggacaa atccattctc ttatatttaa
    10981 gtccttcact tcctcgtttt tatacccaca tttattgcat atttgtgaac ttgcaaagaa
    11041 tttacctact tttacaattt ttctaccatg ccaattagcc ttgtattcta attgacgaat
    11101 aaactctgac catgatacat cagaaattaa gcgagatagt ctacgatttt taatcatatt
    11161 ctttacttgt aaatcttcta tgcaaataat atcattctct tttattaatt tagtagataa
    11221 tttttgtaga aaatcatttc tttgattagc tatatgttct tgaagtct
//
LOCUS       DAELSY010000134        11107 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.134, whole genome shotgun sequence.
ACCESSION   DAELSY010000134 DAELSY010000000
VERSION     DAELSY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11107
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.134"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            367..762
                     /locus_tag="KPG09_002998"
     CDS             367..762
                     /locus_tag="KPG09_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967586.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNNIKCEEIKDNVIEEKNNTCTTE"
     gene            805..2904
                     /locus_tag="KPG09_002999"
     CDS             805..2904
                     /locus_tag="KPG09_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBG0967587.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEISVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLKKDATEILVPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGEAMVNQASLTGESLAVNKKEGSYIYAGTGIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNHSIAVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKKDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLREYGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVSGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYY"
     gene            complement(3129..8339)
                     /locus_tag="KPG09_003000"
     CDS             complement(3129..8339)
                     /locus_tag="KPG09_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBG0967588.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRNFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDRQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKTQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLCIPEGIYSINVSGK
                     DAEKLVGEGSIYDGMNGRNGNNAGTSGDILLSFKEIEKRGELKLYVDGGRGGNGQDGK
                     RGDTGITGEAGENAKIGSKKESKEFAKVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKASLLKGAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSAPIDDSDRNYYYFDRYVFNHASNENQVADDGEKLNALYEKHKDWTISKREDSGLV
                     GAEGKDGRSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFIGEKKNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKDELENK
                     MLSVNAKMNLLIQEIIKMKEEEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYAEKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSGSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYIDI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSESIDILKSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIC"
     gene            complement(8354..10477)
                     /locus_tag="KPG09_003001"
     CDS             complement(8354..10477)
                     /locus_tag="KPG09_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0967589.1"
                     /translation="MQNSVKQEPCDFSHGRFRPEADILDSRLTKVLDLFQQLNMCLHK
                     DNFRGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFEELLKDNGK
                     NIHLCKPLESDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIKEIQNNIVS
                     SLEDYVKAYTEDFISIIGSITMIQEIAKYDEVVKVFKNWLESTTTLTIERFINVLAKQ
                     SGLFLQDSCMELQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEKASKKIMEQ
                     NFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSILTNVTVTQ
                     NEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIRALIPNLVI
                     SSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMDFASEKNIILACNNLDVGSNVKI
                     NVSGKSGELVDSSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNGSKGIDGKN
                     GETGDSGSNGADAGYTSSFHGQMFKGYFAKGTSGKKGDKGGAGGNAGRGGNAGNKGNI
                     YFISDSEEQKNRIINHCDITVKDGEAGPNGRPGSGGYGGSGGYHGYNTLSFSPSGIDK
                     TVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDDGGFNAPNT
                     KLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(10542..>11107)
                     /locus_tag="KPG09_003002"
     CDS             complement(10542..>11107)
                     /locus_tag="KPG09_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0967590.1"
                     /translation="LGIKEFCISSCGDFIENPKYLKKSLNKLAKLQRELSRKTIGSLN
                     RNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIKNRKLSRLIS
                     DVSWSEFIRQLKYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLSIREWICPSC
                     NETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         3572 a         2062 c         1297 g         4176 t
ORIGIN      
        1 tataagctat ataaagtatt acctcaacta tataagctag atatataaat tacatacata
       61 agctagatat ataaattgca tatataagct atataagaat tatttcaact atataaacta
      121 catatataaa ttacatacat aagctagata tataaactag atatataaac tgcatatata
      181 agctatataa aaattatttc aactatataa actacacatg taaaattcat gtaaatttac
      241 taatataaat caaataaaat tactaaactc tctaaactaa ttttgtaaaa catactctat
      301 ttttaacatt tgttacctta tacataagta taaacaaaat ctaactaaat gaaaggataa
      361 tgtaaaatga atattttaaa aatgaaaaaa acaggaatat ttttaggagg agtacttttt
      421 ggtactgtag gtgttaagat actttcaagt gactgtgtaa aaaaaacttg cactcgtgca
      481 acagcaggag cattgaaagt taaagattct gttcttgaaa caactacgaa aattcaagaa
      541 aatgtagaag atattttagc tgaagcaaaa gaaatcaacg agcaagaaga cttatctaca
      601 tctaaggtaa acgttgtaca agatactctt aaatgcgaac aagaagttct taaaactgaa
      661 actataaaaa ctgtagaaga tactaattct aataacatta aatgtgaaga aatcaaagat
      721 aacgtaattg aagaaaaaaa taatacttgt acaacagaat aaatttaatg attattatga
      781 ttaataatat caggaggaag taaattgaat ttttatataa aacacgaaat aagaggtcgt
      841 atacgctttg acttacaact tggcaaatta aacaacaagc agtccgatac tctcttatat
      901 tatttaactt ctataaatgg tattaccaaa gcaaaagttt acgatagaac tggtgatgca
      961 gtcatttatt acactggtga aagagaatac ataataagag aaatcactaa attcagtttt
     1021 aacaatagca aaatagaaaa cctagttcct gaacatacca gcagggaatt aaacaattat
     1081 tatagagaca aattagcaaa taaactgcta gtcaaaagta tctccaaaat attttttcca
     1141 aagtccttaa gatttttttt aattggactt cattctataa aatattttaa aaaaggtctc
     1201 tcttccctac taaataaaaa aatagaaatt tctgttttag atgccactgc tataggtatt
     1261 tctattttta gaaaagatat caatacggct ggttcagtta tgtttcttct tggtatcggt
     1321 gagttattag aagaatggac tagaaaaaaa tctatagacg acttggctca aagtatgtca
     1381 ctaaacattg aaaaagtatg gttaaaaaaa gatgccactg aaatactagt tccaatttca
     1441 gagataaagg aaggagattt agtcagtgta actatgggaa atatgattcc attggatgga
     1501 gttatagtat caggagaagc aatggtcaat caggcatcac ttacaggtga atcccttgca
     1561 gttaacaaaa aagaaggctc ttacatatat gctggaactg gaatagagca aggcaatatt
     1621 gttatgtgcg tcaaggaaaa agctggtact actagattcc agaaaatagt aactatgatt
     1681 gaagaatctg aaaaactaaa atcttctgta gagagtaaat ttgaacatct agcagataca
     1741 ttagttccat acagcttttt aggaagtata ttaacctatg caattactag aaaccctata
     1801 aaatcactct ctatattgat ggtagatttc tcttgtgcat taaaactctc tatccctatt
     1861 tcagtactat cagccatgag agaatgtaac aatcacagca ttgctgtaaa aggtggaaaa
     1921 ttccttgaag gtgttgcatg tgcagatact attgtatttg ataagacagg cacattaaca
     1981 aaagctcaac caactgtttc agatataatt acattccaaa attataataa gaaggatatg
     2041 ttaagacttg cagcttgctt agaagaacac ttcccacact ccatagcaaa tgcagtagtt
     2101 tatgaagctg aaaaacaagg gcttagtcac aaagaaatgc atacagaggt agaatatatt
     2161 attgctcatg gcatttcaac aaaggtcgat gataaaaagg ttgtaatagg tagcaaacat
     2221 tttgtatttg aagatgagaa gtgtactatc ccagacggtg aggaggataa gtacaacaat
     2281 ctatcagatg agtattctca cctatttatg gcaatatcgg gtaaattatc agcagttatt
     2341 tgcataaatg accctttacg aaaagaagca aaatatgtaa tttcaaattt aagagaatat
     2401 ggaataaaaa agattgtgat gatgactgga gatagcgaaa aaacagctaa atctatagca
     2461 agtaaagttg gtgttgatga atattattca gaagtcttac cagaagacaa agccaatttt
     2521 gttaaaagtg aaaaactaaa aggtcgcaaa gtaattatga ttggtgatgg aatcaatgat
     2581 tctcctgcta tttcagaatc agatgtcgga attgcaatga gtgaaggtgc tgaaatagca
     2641 agagagattt cagatattac catatcagca gataatttga ataatttaat cgttttaaag
     2701 cagattagta ataagcttat gaaaagagtt gatttaagtt ctaagtttat tatcggcttt
     2761 aatttaggtc tgattttact tggcgtaagt ggatttgtta gaccaagtac atctgccttt
     2821 ttacacaacg catctactgt tggaataagt ttaaacagta tgacaaattt attggaaaac
     2881 accaatactt ataactacta ttaatcccca ttatataaaa catatgtata taaaaaattt
     2941 ataattcatc ttttaaaaag aaactgtctt aaaaggagct tttttctaat caaaattata
     3001 taaaacatat tttacaaatt ttaaactcaa cttatttcta ttttttacaa aaaggagatg
     3061 attcaaattt tcattttgaa acatcccctt ttgtaaattt ctttaaaact tatatattaa
     3121 ttatattttc aacaaataat tgtattttca accaatcttt cttcatagca ttttaaatca
     3181 ttttgcttta gataattaaa ttcagactct acataaatac cacagcctac caattcaatt
     3241 gtcaaattag gtatattttc ctttagtata ctagtctgct cataaataat tctttccaat
     3301 aaatcaactt gtaatataga aaaatgcttc ataatctccc catcactatc tatatctttc
     3361 ttgatttcat accaactctt tgacaaaatt cccttcaaat caatataccc tagactcttc
     3421 aatatatcta tactctcgct ccatgcatac ttactaggtt tatcaaagta tgattctagt
     3481 ttcttcttct gctcatttgt aagtgaatca taagaaagct tcttattaaa atgtgccttc
     3541 aaagaaatct tagccataga atatggactt ctaccagagt ttaatcctgc atttttgaac
     3601 ttttcaattc ctttatcttc tactgaagac catattgagt ttattgaatc tgaatcattg
     3661 tatttaacat caagaccatg tgtaatatct atatagtttt gtggaaatac atattgataa
     3721 tagacatctt ttttatcatc atatagggag aagtatccac tatcatataa atgaatatgc
     3781 acctttactt ttaaactatc ctctaaaata tttcgtctaa ggttcgggat atttgcatac
     3841 atggttacat attttatggc atcaaagttt ttatttaatg gaacttcaaa actaatttca
     3901 tctcctgtca aaagcttttt cacttcttct tcattacaat catatagaga atatgtattt
     3961 tcaaatccca cacgtgattt cccatctaaa ctgcctgtta atgctacctt caaaatatgg
     4021 ttatccgttt tttgccattg gtaagcatct tggtcaagaa actctctaat ctgtctattt
     4081 tgctctttcg cctgatttat aacctcgttt tcatcttctg taatacctac atggcatcta
     4141 tttaagatat ttactatatt gcttccaaat ggaaacgtcc atagttgaaa agctgctgcc
     4201 tcaacctgac acaaatatct aacctgattt aattcaattt gatggataag ttttttctga
     4261 tattcattca tctgattacc actacctgac atttgataaa tcaattctcc cattgcaaga
     4321 ttatcttttt tcttatcaat cttatcagta atgataattt gagtttccat catatcctgt
     4381 acttttttaa atattataga aatctcctct gaattttcca tttttaagct tccaaacttt
     4441 gtgcttaatt cagttaaaac ttccttcatc tttaatttac ttatctcatt ttcaaaaata
     4501 tcttgatttt taactgtact atcgaatttt tctacaaatg gtataacaga ttctttcaaa
     4561 gttttatctt taaattccac catttgagta ttcaattcat ctaatttctt aatattattt
     4621 tctttatctt taattgcctc tttaactacc tctattttac tctcaaaact agattttaca
     4681 ttaagacaat tattaacacc actaatcgca gtatttataa gagaacaagc agaacttcca
     4741 atactcaatg catttttaat tttagatgtc ttttgctgtc tttcaatagc ttgtttactt
     4801 ttctctagtg cagctaactt atctttttgc tgtttattat tgtaatttgc ctccaacata
     4861 cctatttctt ttttctggat ttttgtgtaa atttccttat actcgctttc acttaacccc
     4921 tcttttgctc tttgaatatc gtcagctttt tctgcatagt tatcatcatc tatagactta
     4981 actataatct ccttaatttg gtcaaattta gttcttcttt cttcatcaac acctgccaaa
     5041 ttataagtca ttttatctaa agcatttttt ctatcttcac ttagctgttc tccaagtaca
     5101 aatctcatac catcagaatc caatttaaaa tctgaaaaat caaccatcct gttagtccat
     5161 tggtattcct cttctttagc tgaaagattt tccttcaaag attttgcact gtcaaatgcc
     5221 cctagacaag cttgaccaac accactaaat gcagtcagta aactcaatgc gctttgtatt
     5281 ccagaaagaa ttaactgtgt tttcatttgt tcctcaagtt ttgcctgttt cttttttagt
     5341 tcctctatgt cttcttgctc ctcttctttc atctttataa tttcttgaat caacaggttc
     5401 atctttgcat taacagaaag cattttattc tcaagttcat cttttaaaac ttctatttgt
     5461 tgatttccag attcaatttg agttaggata ctttccttat aagtattgaa atatccttct
     5521 tttaactcat ctttctttcc ttgttccaat tctcttaagg tcttgtccag ataatctgtc
     5581 aaagaacgaa tatctgtaac acgatttcct ccaatttcac tttcttcaat catctgcaca
     5641 tagaatgctg ttttttgttc aagtgcaaat aaaatcatac tccattcgct aaaattcttc
     5701 ttttctccta taaaagattc tattctatca gactgcatca atttatttat aaggtattca
     5761 tagatttcct ttgcctcctt ataaaattct ttctttatat tcatatcatc cagagaaagc
     5821 actgctttat ttttcttctc aaaactatag tacttctgaa gtatttctct atttaattgc
     5881 atctctattt tctcaactgc atgaaaaatt tcataaatat gtgaaagttc taaaatatca
     5941 gcttctaaaa ttcccactgc acacaacagt ctaagatttc tataaccttt aattccagaa
     6001 aaaccaaatc tctcgaaaaa ttcatagaag aaggtatcta ttgacaaaga atactcctgc
     6061 tttaacaatt ccttttcttt taatgcatct ccagagcgac cgtcttttcc ctctgctccg
     6121 actaaacctg aatcttcacg tttgctaatt gtccagtcct tatgtttttc ataaagagca
     6181 tttaatttct ctccatcatc tgctacttgg ttctcatttg aagcatgatt aaacacatat
     6241 ctatcaaaat aatagtaatt tctatcactg tcatctattg gagcagaggt acctatatat
     6301 cttattgttt cttcatatat acctctaatt ccaccaagac ctcctgcacc accagctcct
     6361 ccagagcctc cttttcctgc tagaccatga tttccatctt ttttacttat agcacctttt
     6421 aataaagaag cttttccaag gagataaacc tgtccagact tgctcccaag acctgcattt
     6481 ccaccattac ctcctgtgcc tccagttcca ccttgttttc ctttttctgc ttttaccaca
     6541 tagtattttg tacctttaac cttagcaaat tcttttgact cttttttaga acctatttta
     6601 gcattttccc cagcttctcc agttatccct gtatctcctc tttttccatc ttgtccattt
     6661 ccaccacgtc caccatctac gtaaagttta agttctcctc ttttttctat ttctttaaaa
     6721 gataataaaa tatcacctga agtacctgca ttattgccat ttcttccatt cattccgtcg
     6781 taaatagaac cttctccaac caatttttct gcatccttac cagatacgtt gattgaataa
     6841 atcccttctg gtatacataa tttttctgta agtagtacaa tattcttacc tgagaagact
     6901 tctttctcca tattacagtc aattttcaac tcacaatctc catacatata tatagatgac
     6961 aaaatattaa gttgataacc agatattatt ttagtcaaat ccgataaatt cactttaggt
     7021 ataggaatat atatagaaag tacattttct ttctcatatg attttatata tgtacaactt
     7081 tgaatcttct ctatcctata ctctgcatct tcaagctttt tggcttcttc ttccctatat
     7141 tcttcttctg ctatccaata tttgatatct tttaatgtat cttctaaagt attttttagc
     7201 aatgatttta acaactcact tagaccaaca atatgagtct cactacagta gctatttaac
     7261 tctaaaagaa gacgtgtttc ttctaatata ttcactttct gtacgccatc accatatgaa
     7321 agttcttgta caaactccat aagctctttc atattattag tcctactaca taaatctgta
     7381 tacatcttat ctaattgagt ctttttatcc tttagactat ttatagaaga tagaatggaa
     7441 atagaatctt ccataccttt tttgacactt tctatataag gaactaaaag tttttctagg
     7501 tctttatagg ttacatcaac ggctttttgt atttctattt tacaagaagc agtaagcatc
     7561 aatcccaaat ctgttttttc aaatgtttca ggtagatttt tcatttcatc caacaacttt
     7621 tctggctgtc tatctagtgg attacataat ttaatagata caccatctga taatgctaca
     7681 tttaataagt acactaattc cttacaaccg tcgtaatctt tctcttgact atctaggtct
     7741 tcttttgctt tttccaatat attccttatt ttttcttctg ttacattacc gattagtgtt
     7801 ttaggtattt tattgataag aaagaagaaa ttttctttat actttgcata atcaccaagc
     7861 atatctttca aatttttgcc tgtttctatc aataatgtat gtctagagtc cagtaaggat
     7921 tgatagtcta aaataactac catccctttc acctttgaag atgtagaaat agtttccctt
     7981 acaagcatag caccagcaat catatactct gctccacggt tatcaaaaaa acctgggcaa
     8041 tcacataatg aaaaatttct atttgcaata tctacaactt ctggacataa agtcttagaa
     8101 tatacaccat ttccaatttc gacagctgaa tcttttgcag ttatatatat ttgtccagtc
     8161 tcttcatccg ttttttcttc catcacgcaa ccttttaaat aatttataca agtactcttt
     8221 ccaacgcctg aagcacctaa gaataacaaa attgactttt cttttaagtc atattttcta
     8281 agctcgtctc ttccttcttc caaaaggttt acgatttcac tataaagttt atttgccata
     8341 catccatccc tttctatatt tcggaacaat agccttgata tttttcttta ataagattct
     8401 cttctacctt tgaaacagct gttgataact tagtatttgg agcattaaat cctccgtcgt
     8461 ctcctctatc tccatattca gcgtattctc ttgctggtcc tgttttgcct tcattccaac
     8521 ttccgccact aaattctata tgatatttaa ttatactatc taacttataa tcccaacttg
     8581 taaaatatcc tgtatagtaa acagtcttat ctataccact tggagaaaat gaaagtgtat
     8641 tatatccgtg atagccacca cttcctccat atccacctga acctggtcta ccatttggac
     8701 ctgcttctcc atctttcact gttatatcac aatgattaat tattctattt ttttgttcct
     8761 ctgagtctga aatgaagtaa atattacctt tattaccagc atttccacct cttccggcat
     8821 ttccaccagc tccacctttg tcaccttttt taccacttgt accttttgca aaataccctt
     8881 taaacatctg tccatggaaa ctacttgtat atcctgcatc agctccatta cttccagaat
     8941 ctcctgtttc accattttta ccatctattc ctttagaacc atttcctcca tttgcaatca
     9001 gtgttaaacg ataatttcta atttcttctt taatatataa gtaaatatta cctgcatctc
     9061 taccatcttc accatttttc ccatacatag aactatctac aagttctccg ctctttccac
     9121 ttacattaat ttttacatta ctacctacat caagattgtt acaagcaaga ataatatttt
     9181 tctctgatgc aaagtccatt ccaaggttat catccatata aatagtatct ttggcaaaga
     9241 acataaatgt agaaacattg tcatctactt ttctattttt gaaatattcc aatacactac
     9301 taattactaa gtttggaatt aatgctctta tttcaatacc tttcttatca ttaatattaa
     9361 ttgtttttac tataaatgga tttaaaatgt atttaccaat aaattctcta attatctcat
     9421 taccttcttc tccatatgca cttaatcttg ttatactgtc ttctaactct tcattttgtg
     9481 taactgtaac atttgtcaaa attgaattaa aatcacttac actatataca taatcctttt
     9541 tatttgcaac ttccattaat ttttctctat ttggtaatgt aaatacctgt ttttgtactt
     9601 cataagaaac taaaactttt tctaaattct ttcttattat aatgaagttt tgttccatta
     9661 tcttcttact tgccttttct ataataaatt ccatatgtac agaaggtaat aaaatttccc
     9721 cagttccatg ttctgtatat ttacttaaag catcaattat atctaagttt ccttttatct
     9781 tattttgtaa ttccatgcaa ctatcttgta agaataagcc actttgtttg gctaatacgt
     9841 tgataaatct ctctatagta agagttgttg tactttccaa ccagttttta aatactttta
     9901 caacctcatc atattttgct atttcttgaa tcattgttat acttccaata atactgataa
     9961 aatcttctgt atacgcctta acataatctt ctaggctaga tacgatattg ttttgaattt
    10021 cttttatcaa cgtatctaaa actaatcttg catctgcact aattggatat tggaatgctg
    10081 atgtcttatc tttaacttca tctaattcta gtacttgctt taataagcct tccctctcgt
    10141 ctgattcaag tggcttacaa agatgaatat ttttaccatt atcttttagc aattcttcaa
    10201 ataaccaagc tttttcctct atattactat ctaaattttc acataattgt tcatattttc
    10261 tttttataac attatatatt tgtttttctg ttcttcctaa gaaacttttt gaaatgaaaa
    10321 aagttatact gcccctaaaa ttgtctttat gaagacacat atttaactgt tgaaacaggt
    10381 ctagtacttt tgtaagtcta gaatctaata tatctgcttc tggtctgaac ctcccatgac
    10441 taaagtcaca gggttcctgc ttcacagaat tttgcatact aaaatatgtc ttactaattc
    10501 tccacaggcc atccccgtag ttcctacggt tcttacataa gttatttatt ttgaattgtt
    10561 attagtctta atccttcttt taatatattt atacttgcat ttatatctct atcgtgagtt
    10621 tcattacagc taggacaaat ccattctctt atacttaagt ccttcacttc ctcgttttta
    10681 tacccacatt tattgcatat ttgtgaactt gcaaagaatt tacctacctt tacaatttgt
    10741 ctaccatgcc aattagcctt gtattttaat tgacgaataa attctgacca tgatacatca
    10801 gaaattaagc gagatagttt acgattttta atcatattct ttacttgtaa atcttctatg
    10861 caaataatat cattctcttt tattaattta gtagatagtt tttgtagaaa atcatttctt
    10921 tgattggcta tatgttcttg aagtcttgca acttttaatc ttgctttatt tctatttaaa
    10981 ctaccgattg tttttctcga taattctctt tgtaatttag caagtttgtt taatgacttc
    11041 ttaagatact taggattttc tataaagtct ccacaacttg agatacaaaa ttctttaatt
    11101 cctaaat
//
LOCUS       DAELSY010000135        11103 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.135, whole genome shotgun sequence.
ACCESSION   DAELSY010000135 DAELSY010000000
VERSION     DAELSY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11103
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.135"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            351..1673
                     /gene="dnaA"
                     /locus_tag="KPG09_003003"
     CDS             351..1673
                     /gene="dnaA"
                     /locus_tag="KPG09_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBG0967591.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            1915..3021
                     /locus_tag="KPG09_003004"
     CDS             1915..3021
                     /locus_tag="KPG09_003004"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBG0967592.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            3158..3364
                     /gene="yaaA"
                     /locus_tag="KPG09_003005"
     CDS             3158..3364
                     /gene="yaaA"
                     /locus_tag="KPG09_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBG0967593.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVKIEGTKIKVL"
     gene            3382..4497
                     /gene="recF"
                     /locus_tag="KPG09_003006"
     CDS             3382..4497
                     /gene="recF"
                     /locus_tag="KPG09_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBG0967594.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            4498..6399
                     /gene="gyrB"
                     /locus_tag="KPG09_003007"
     CDS             4498..6399
                     /gene="gyrB"
                     /locus_tag="KPG09_003007"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBG0967595.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            6419..8845
                     /gene="gyrA"
                     /locus_tag="KPG09_003008"
     CDS             6419..8845
                     /gene="gyrA"
                     /locus_tag="KPG09_003008"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBG0967596.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKIGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKNEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            8914..9210
                     /locus_tag="KPG09_003009"
     CDS             8914..9210
                     /locus_tag="KPG09_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG0967597.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            9210..9470
                     /locus_tag="KPG09_003010"
     CDS             9210..9470
                     /locus_tag="KPG09_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBG0967598.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            9482..9799
                     /locus_tag="KPG09_003011"
     CDS             9482..9799
                     /locus_tag="KPG09_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBG0967599.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVKKIFTITGLDKIFK
                     VEG"
     gene            9824..10210
                     /locus_tag="KPG09_003012"
     CDS             9824..10210
                     /locus_tag="KPG09_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0967600.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            10210..10983
                     /locus_tag="KPG09_003013"
     CDS             10210..10983
                     /locus_tag="KPG09_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBG0967601.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
BASE COUNT         4519 a         1151 c         2016 g         3417 t
ORIGIN      
        1 cattgaagtt tgacatatat tttgatatta taaatatact tgttgataat gtgcataaat
       61 gttatcaact tattagaaat tattcacagc ttgtggatac ttttgtgtat aacttttaag
      121 attactgaca atactctagt tataatcacc atttattgta tgtttatgtt atttataact
      181 ttatctacat attgtttata tcttttcata tttttttaat aatttatact attattaatt
      241 tatcaacacc attatccata aatttagtta ttctgtaaaa tttatatata tttttgcttt
      301 tctgttaata actttgtgga aaatattaat tattctgttc ggaggtttat attatggata
      361 tagtttcttt atgggacaaa accctacaat taataaaagg tgacttaact tcagtaagtt
      421 ttaatacctt ttttaaaaac atcgtaccat taaaaataca tcttaatgat ttaatcttac
      481 tggctcctag tgattttaat aaagatatct tagagaatag atatttacat ttaattgaag
      541 atgctattag tcaactgtca ttaaaaaaat ataatattaa atttgtattg agcgaaaaag
      601 aggttgctga tttaaattct gatagcacag atttaaatta tagagttctt taccccaatc
      661 taaatccaaa atacacattt gatacattcg ttattggtaa tagcaataga tttgcacatg
      721 ctgcatgcgt tgcagttgca gaatctccag ctaaagctta taacccactt tttttgtatg
      781 gaggagttgg attaggtaag actcacctaa tgcatgctat aggtcatcat attgttagtc
      841 aaaaaaaaga ctcaaaagta gtttacgttt cttcagaaaa atttactaat gaacttataa
      901 actctataaa agacgataaa aacgaagaat ttaggaataa atatagaaac gtagatgtat
      961 tactaatcga cgatattcaa tttatagcag gaaaagaaag aactcaagaa gagtttttcc
     1021 atacattcaa tactcttcat gaagcaaaca aacaaataat tatatctagt gatagaccgc
     1081 caaaagacat acctacacta gaagataggc taagatcgag atttgaaatg ggacttataa
     1141 ctgatattca agcacctgac tttgagacaa gaattgctat acttagaaaa aaggctcaac
     1201 tagaacgtat agatgtgcca aatgaggtta tgtcatatat agcaaaaaat attaagtcaa
     1261 acataaggga actagaaggt gcattaacta gagtggttgc ttattcttct cttagcaata
     1321 gggtaatatc ttttgatctt gctacagaag ctttaaaaga tattataaca acttctaaaa
     1381 atgaagaaat aaatgttctt agaataaaag aaaaagtatc ttctgtattc aatttaaaga
     1441 tggaagattt taattctaaa aaaagaacac gttcaatagc ttatccaaga cagatagcta
     1501 tgtatttaac gcgcgagctt actgatttat cccttcctaa aataggtgaa gagtttgggg
     1561 gtagagacca tacaacagta attcatgctc atgacaaagt atctaaggat atagaagaaa
     1621 gtgaagaaat taagactaaa atagacaaaa ttatatcaga tttgaaggga taatttatac
     1681 acaattgtat gtttatatct cttgaataac ctgttaatac tttttttgaa caaaaattat
     1741 taacaaatta tcaagtctaa aataacaaag atattaacat cttatacaca tgttaatatc
     1801 tttgtattta taatgttata catacttatt aacattatca acaacaccta ctactattac
     1861 tactatgatt ttaacttatt tttatatata aaaagccaga ggaggttatc cacattgaaa
     1921 ataatttgta atcaaaaaat cctagcaaat agaattggaa ttgctcaaaa agctattaat
     1981 ggaaaaacaa caatagaatt attaaaaggt atacttatat ctacagaaga aggtcaatta
     2041 aaacttactg gatatgatgc tgaaatagga atagaaactt atgtacaagc agaaatcata
     2101 gaaaaaggtg atgttgttgt agatgcaagg ctatttggag atataattag aaaattacca
     2161 gattcctttg ttgagataga aactgattca gaaaataata tttacataaa ttgtgtaaat
     2221 tcaagattta aaattaaagg ttatgcagca aaagaatttc ctaaattacc agaattaaat
     2281 gaagaagatt tatatagcat acctcaagaa attctaaaaa atatgattaa acaaactgta
     2341 tttgctatct cacaggacca aacaaaacct gttttaatgg gagaactttt agaaattgta
     2401 gatagaaact taaatttagt agcaattgat ggatatagat tagctgtaaa aagttgttca
     2461 gttgatagtt taactgaaaa tataaaagtc ataattcctg gaaagacact tatagatgta
     2521 aatagtttgc tttctggaga ggataatgtt aaagttgggt ttaacgaaaa aaatgctatt
     2581 tttataatta atgatacgaa aattattaca agattgcttg aaggagattt tattgattat
     2641 aaaaaattat tgccaagaga acataacagc agagttaaat taaacactaa ggaactttta
     2701 aatagtatag agagagcgtc tttattgtct caatcagaaa aaaataatct tataaagtta
     2761 tctataagag ataaagtaat ggcaattact tctaacacag aaaaaggaaa tgtgtatgaa
     2821 gaagttgaaa tagatttaga tggagattac ctagatatag ctttcaattc tagatatttt
     2881 atcgaaggtt tgaaaaatat agataatgag gaaatattta tagaatttac tacaaatgta
     2941 aatccttgta taattaaacc aacagatgat gttaattata tttacttact gcttccagta
     3001 agaatatcat caaatatata gcagacatga aggagttgtc ttttgtagaa aatttttcta
     3061 atcttagaca actcctttta taagcatatt tatggtataa tatactttta gtgtatcaat
     3121 aggaatttgt ttaaaatagt gacaaggagg aactattatg actgaaataa ctatagaatc
     3181 agaatatata aagttggatc aatttcttaa attagcagag attgcatcaa caggaggtca
     3241 tgcaaaattt ttaattcaag aaggtttagt gacagtaaat gatgaaatag aattaagaag
     3301 aggaaaaaag ataaaatcag gagatatagt taaaatagaa ggaactaaaa taaaagtatt
     3361 gtaaaaaaag gagaactttt tgtgaaactt aaaagtttgc aacttgttaa ttttagaaat
     3421 tataaaaagt tgcatctgga atttaatggg aaagtaaatc tacttgttgg taaaaatggt
     3481 caaggaaaga caaatatagt agaatctatt tatatgcttt cttttggaaa atcatttagg
     3541 actaacaaag ataaagaaat ggttagattt aatagtgaaa acttatacat tggaggaagt
     3601 ttttcaaaat ataataaata tagtctaata gagctaatta ttggaaaaga taaaaagggg
     3661 ataagaataa ataaagttcc tttacaaaag atacaagaac tacttggcaa cttaaatgtt
     3721 gttatatttt ctcctgaaga tttaaggttg gttaaagagg gtcctaaaga aagaagggca
     3781 tttatagaca aagagattag tcagattata cctaaatatt ataaatatct taccaattat
     3841 aataaaacac tttctcaaag gagtagggtg cttaaaaata tacatgttga tgaagcttta
     3901 ttagatgtat atgatgatac gttagcaaag tatggaagtt atatttatat tttgcgtaga
     3961 gactttataa aaaagatagc taatatatct gaaaatatgc atatgaattt gactaatgga
     4021 gttgaaagat tatcaattcg atataaaaat caaataaata taactgatga agatactata
     4081 gatacagtat ataataaatt tttagccaag ttgtcatcga atagacctaa tgatatagaa
     4141 tctaagacta ccagatatgg tatacacaaa gatgacttaa atatattcat aaatgattta
     4201 gatgcaagac tttttggttc acaaggtcaa caaagaactg cttcaatatc attaaagtta
     4261 tcagagatag aattaataaa aaatgaagtt gaagaatatc cagtattaat tttagatgat
     4321 gtttttagtg agttagatga agcaagacaa aaacttttag taaataattt aagcaatgtt
     4381 caaatgttta taacaagtgc tgaagtttca cataaaaaaa tatttgatga aaaaaatgtt
     4441 acaatattta atattgaaaa tggcgatgtt attagcatag agaatggagg aaattaaatg
     4501 aaacaagaat acggtgcaag tcagatacaa gtattagagg gattagaagc cgttagaaaa
     4561 agaccaggta tgtatattgg tagtacaagc cctagaggtt tacatcactt agtttatgaa
     4621 gttgtagata atagtataga tgaggcttta caaggatatt gttcagacat atatgtatct
     4681 attaatgaag atggcagtgt tttagtaaaa gataacggaa gaggtatacc tgttgaaata
     4741 catcctaaaa ctggtaaatc aactttagaa acagtattaa ctaatcttca tgcaggagga
     4801 aagtttggag gcggaggata taaagtatct ggaggtcttc atggagttgg tgtttctgta
     4861 gttaatgcat tgtcaaaatg gatggtagca gaggtttatt taaacggaaa gatatataag
     4921 cagacatatg agaaaggatt accaacatct aagcttgaag tagttggaga atctcaagat
     4981 aaaggaacaa tgattcaatt tatgccagat gagacaatat ttgatgaaat agagtttaaa
     5041 tatgaaacat tggagtatag gcttagagaa ctttctttct tgaataaagg aataaaaata
     5101 gtatttgaag ataaaagaga aggacaagaa aaaagaaaag aatttcacta tacaggtggt
     5161 ttggtagaat atattaagta tttaaataaa tctagaactg gtatacatga tgacatagtt
     5221 tatatagata aaaaggttga tgattgtttt gttgaactag ctatgcaata tacagatggt
     5281 tatacagaga acatatattc ttttgcaaac aatataaata cacacgaagg tggttctcat
     5341 ttaagtggat ttaaagcagc tcttactaaa actgtaaatg attatgctaa gagaaataaa
     5401 tttttaaagg aaaatgatgt taacctactt ggtgaagaca taagagaagg tcttacagca
     5461 gtagtatcag ttaaattacc agaacctcaa tttgaaggtc aaactaaaac aaagctaggt
     5521 aatagcttta tgagaggtat agttgatagt gtaacagttg atgaactggg gtctttccta
     5581 gaagaaaacc catcaacagc aagaatcata gttgataaag ctttaagagc acaaagagct
     5641 agagaagctg caaaaaaagc aagagaatta acaaggagaa aaagtgtatt agaaagtaca
     5701 tctttacctg gaaaacttgc agattgtgca gaaaaagatc catctaaaag tgaaatattc
     5761 ttagtcgaag gggattcagc gggaggttca gctaaacaag gtagagatag aaatagtcaa
     5821 gctatacttc cattaagagg gaaaatactt aatgttgaga aatcaagact agataggata
     5881 ctatcttcag atgaaataaa aaatatgata acagcttatg gttgtggtat tggagaagat
     5941 tttgatatag ataaggctag gtatcataaa attataatta tgaccgatgc tgatgtagat
     6001 ggagcccata taagaacttt attattaaca ttcttcttta gatatatgag acctcttata
     6061 gatgaggggt atgtttatgc agcacagcca cctttatata aagtgacaaa gcaaaagaag
     6121 gaacattatg tttactcaga taaagagtta aatatcttat tagatgaaat tggaagaaat
     6181 ggtgttgaac ttcaaaggta caaaggtctt ggagagatga atgcagagca attatgggaa
     6241 acaactatga atcctgagac aagaacttta ttacaagtta ctgtagaaga tgctgcaatt
     6301 gcagatgaag tattttcaat gcttatgggt gataaggttg ctccaagaaa agaatttata
     6361 gaagaaaatg caagatttgt tagaaattta gatatataga gagaggggat tagtatacat
     6421 ggaagaaaat aacaaaatac tccctattga aatagcggaa gaaatgaaaa aatcgtatat
     6481 tgattattca atgagtgtta tagctggacg tgctcttcct gatgttagag atggtttaaa
     6541 gccagttcat agaagaatat tatattcaat gagtgagtta aatttaactc cagataaacc
     6601 atacaggaag tcagctcgta ttgttgggga cgttttaggt aagtaccatc ctcatggaga
     6661 tactgctgtt tattatgcta tggtaagaat ggcacaagat ttttcaacta gagcactttt
     6721 agtagatggt catggtaact ttggttctgt tgatggggat tcaccagctg ctatgcgtta
     6781 tacagaagct aaaatgagta aattatcatt agaactactg agagatattg aaaaggaaac
     6841 tgtagacttt aaaccaaact ttgatgagtc gttaaaagag ccttcagtat tgccagctag
     6901 atatcctaat ttattagtaa atggctcaaa tggtatagct gttggtatgg caacttcaat
     6961 acctccacat aatttagcag aagtaattga tgcaactgta tatttgatag ataatccaga
     7021 gtgtagtgta gatgatttaa taaaatttgt tcaaggacca gatttcccta cagctgcaat
     7081 tataatggga aaagaaagta tagcagaagc atacagaact ggaagaggaa aagttaaagt
     7141 taggtctaga gcttttatag aagaactacc aaaaggaaaa cagcaaataa tagttacaga
     7201 aataccttat caagtaaata aggctaaact ggttgaaaga atagcagagt tagttaaaga
     7261 aaagagaata gaaggtatat cagacctaag agacgaaagt aatagaaatg gtatgagaat
     7321 tgttatagaa ttaaagaggg atgctaatgc taatatagta ttaaataatt tgtataaaca
     7381 ttctcaaatg gaagatactt ttagtataat aatgcttgca cttgtagatg gtcagccaag
     7441 agttttaaat cttaaacaaa tattatatca ttatattaaa catcaagaag atgttgttac
     7501 aagaagaact aaatttgaac taaataaagc tgaagcaaga gcacatattt tagaaggatt
     7561 aaagattgct ttagataata tagatgctgt tataagcttg ataagagctt caaagactgg
     7621 gcaagaagct aagataggtt taatagaaaa attcaaatta actgaaatcc aagcacaagc
     7681 tatattagat atgagacttc aaagacttac aggtttagaa agagataaga tagaagctga
     7741 atatgaagat ttaatcaaga agataaatag attaaaagaa attttagctg atgaaagatt
     7801 acttttaaat gtaataaaga atgaaattac aataataaaa gaaaattact ctgatgagag
     7861 aagaacagaa ataagacatg ctgaaggcga aatagatatg agagatctta taagtgatga
     7921 agaaatagca ataactctta ctcactttgg atatataaaa aggcttccat ctgatactta
     7981 taagagtcaa aaaaggggtg gaagaggtat ttcagcactt acaactagag aagaagattt
     8041 tgtaaggcac ttggtaacta caactactca tagtagacta ttattcttta caaataaagg
     8101 tagagtattt aaattaaatg catatgaaat accagaaggt aagagacagg ctaaaggtac
     8161 tgctatagtg aatttacttc aattgtcagc agatgagaaa attgctactc taatacctat
     8221 tgatggtaat gatgaaaatg aatatttatt acttgcaacg aaaaaaggta ttgttaaaaa
     8281 gactaagaga gaagagttca aaaatataaa taaatctggt cttattgcta taggtttaag
     8341 agatgatgat gagcttattg gagtagaact tacagatgga aaacaagaag tacttttagt
     8401 aactaaagaa ggtatgtcta taagatttga tgaaaatgat ataagatata tgggtagaac
     8461 agcaatgggt gtaaaaggta taactttaag taaagaagat tttgttgtgt ctatgaacct
     8521 ttgcagtaaa ggtacagatg tgttagttgt aagtaagaat ggttttggaa agagaacaaa
     8581 tatagaagag tatagaagtc aaataagagc tggtaaagga attaaaactt ataatatatc
     8641 tgaaaaaact ggtacaattg taggtgcaga tatggtcaac gaagatgatg agataatgat
     8701 tataaattct gatggagttc ttattagaat aagagtcaat gaaatatcac tgtttggaag
     8761 agttacaagt ggtgttaaat taatgaagac gaatgatgaa gtcaatgtag tttcgattgc
     8821 caaaataaat attgaagaag aataggataa atatcctatt ctttttgtat aatgtagtta
     8881 gataaaaatt taaaattgaa aggagtaatt gttatgtgca ataaaagaag ttgtaaaaaa
     8941 ggtggatttt tattattagg agctatttta gggtttatat ttggtatgtt ttttgcacct
     9001 aaaaaaggtt ctgaactaag aaaagaaaca aaagagaagt ttaatgatgt caaagaaaat
     9061 ccaaaagaag ttctacatga aacatttaat gatgttaaag agagaataat aaatttagtg
     9121 gacgatgata ataatgaaga agatataaag atttcagaag aagatatagt aataagtaaa
     9181 agctttgatg atgagggaga tgttaactaa tgaatgcatg ggggtggcaa attggagcag
     9241 tgttagtagg aagctctgct cttatagtag caatttattt agctaaaact ctaaacagca
     9301 taaataaggt tgttgaaaag gcatataaga ttgtagatta taatgaaaga catatacaag
     9361 atattgttga aaatgcatca tcaatatcaa agggaataga tgatatagtt tatgtaatta
     9421 ataaaatatt gagtatagga aatatattta aaattattaa aaggaaatag gaggttttta
     9481 catgtctatg aatattgatt caaatttaga ttcacaaaat aaattttgga atgtttgtct
     9541 tgatggagaa cttgatgttt ctacagcaga taaactaaag gaacatttac atgctttaat
     9601 agaaaaaaat atgttagatg taaagattaa tcttaaagat ttagattata tagactcaac
     9661 aggcttagga gctatgatag gtgtattaaa aaaattaaaa ataaatgaaa aagagatata
     9721 catagtaaat cctaagagta acgtaaaaaa gatttttact ataacaggtc tagataaaat
     9781 atttaaagtg gagggataaa ctttggcttg tgagactata aagatggaaa ttactacaaa
     9841 tccagattat gttagtataa taagattaac agcatctgga attgccaata agatgggttt
     9901 tccaatagat gatatagaag atataaaggt agcagtatct gaagcatgta caaatgcaat
     9961 aaaacacagt aaagatgatg tgtttaatat agtatttaat attttagata atgcaattaa
    10021 tatagaaatt caagataatg gaaaaggata tgatgttagc tcaataagta caccagattt
    10081 agagaatcca aaagagagtg gtcttggatt gtttataatt aaaactttaa tggatgatgt
    10141 agatattgaa tcagaacata atcaagggac aaaaataaaa atgactaaat atttaggagt
    10201 tgatatttaa tgaaaaatgt agctaatgct acacattacc taaatatgga tacaaaagag
    10261 ctatttaatt tatataataa aaataaaaat gtagacatta gaaatattct tattgaaaga
    10321 catctgtatt tagcaagatt gttagctaag aagtatataa acaaaggtgt tgattttgaa
    10381 gatatatatc aagttgcttc attggcatta atttatgcta ttgatagata tgatgttgag
    10441 aaaggatttg agttctccag ctttgcgaca ccaactatag taggagaaat aaaaaagtat
    10501 tttagagata aggtatggac acttagagtc ccaagaagaa tacaggaatt aagcaaaaag
    10561 ataagtgatg cgaagattaa attagaacaa gaaaataaga agcatccaaa ggtaaaagat
    10621 atagctgatt atataggcgt tagtgaagaa gatgttttag aggctatgga ggcttcttat
    10681 ggatatcaac caatgtcttt ggattcatca agtaatgatg attcagagga taaagatata
    10741 actcttattg ataagattgg aaaagaagaa ggtaactttg gaagtataga gtatgaagat
    10801 tttattaata agtttattga aactttaaat gaattagaag ttaaaatttt taaagataga
    10861 tttttctttg ataaaacaca atctagtata gctaaagaac tagggatatc acagatgact
    10921 gtttctagac tagagaaaaa ggtagtagag aagctgaaaa aagaatatga aaaaaattta
    10981 taaaaaaata taaaaaaagt attgacctac tgttttaaag atggtatagt attacttgtc
    11041 cttgaaaaaa ggaacaagaa ataaacacga acattgaaaa ttaaacagta ggttaattta
    11101 tat
//
LOCUS       DAELSY010000136        10794 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.136, whole genome shotgun sequence.
ACCESSION   DAELSY010000136 DAELSY010000000
VERSION     DAELSY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10794
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.136"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            126..590
                     /locus_tag="KPG09_003014"
     CDS             126..590
                     /locus_tag="KPG09_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBG0967602.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            597..1091
                     /locus_tag="KPG09_003015"
     CDS             597..1091
                     /locus_tag="KPG09_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBG0967603.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1107..2132
                     /locus_tag="KPG09_003016"
     CDS             1107..2132
                     /locus_tag="KPG09_003016"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBG0967604.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2119..4566
                     /locus_tag="KPG09_003017"
     CDS             2119..4566
                     /locus_tag="KPG09_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBG0967605.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4746..6119
                     /gene="radA"
                     /locus_tag="KPG09_003018"
     CDS             4746..6119
                     /gene="radA"
                     /locus_tag="KPG09_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBG0967606.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDENIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6122..7192
                     /gene="disA"
                     /locus_tag="KPG09_003019"
     CDS             6122..7192
                     /gene="disA"
                     /locus_tag="KPG09_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBG0967607.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7281..8375
                     /locus_tag="KPG09_003020"
     CDS             7281..8375
                     /locus_tag="KPG09_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBG0967608.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8504..10681
                     /locus_tag="KPG09_003021"
     CDS             8504..10681
                     /locus_tag="KPG09_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967609.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDMSTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSENGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVVFVELGDTYRLDLYKPNLNEKTYESVKDNIEKNIDVYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVLFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
BASE COUNT         4507 a         1093 c         2006 g         3188 t
ORIGIN      
        1 aaattcatat ctaaatttct ataaaaaatt taaatttata caatttaggg ctttaaaaat
       61 ttaaataata ggtgtaaaat aatagtcaaa gaaagtcaaa aataaaaagg tgtgtggtga
      121 tattaatggc aactatgaca gatataattg aaaaatttat taaagatttg atggaagaag
      181 ataatagcat acaaattcaa agaaatgaac tagcaaactt gtttagttgt gcaccatcac
      241 aaataaatta tgtcttaaca acaagattta caatagatag aggatattac attgaaagta
      301 aaaaaggtgg cggaggatat gttcaaatag aaaaaatacg aaaaagtaaa gatggacata
      361 ttagagaact actaaatgaa aaaataggaa gtcagatatc ctataaaaaa gcaaaggaat
      421 tattagaagg tttaaaagag tcggatttga ttaacgaaag agaattaaaa ttaatattat
      481 atgcaataga tgataaatca ttgtgtatgc caatatatga attaaaagag aaggtaaggt
      541 ctaatatttt aaagaatata ataataggat tatttagtat agaggagtga taatttatgc
      601 tatgtcaaaa atgcaataag aataaagcat ctgtatatta taataagata gtcaatggag
      661 aaaaaacaga gatgtattta tgtagtgagt gtgcaaaaga gaatacagaa atgaatttta
      721 acttagatat gcctttttca atgatggata tattttctaa tttaggtttt caacctaaaa
      781 aagaacttga ggaaaagcta gtttgtccta aatgcaatac aacatatagt gagtttaaga
      841 ataatgggag atttggatgt agtgaatgtt ataatgcatt tagtagtcaa gtaaatccaa
      901 tgcttcaaaa tatacatggg catatagaac atacaggaaa agcacctaaa aaatcttttt
      961 ataagataag tgtagaaaat gagattaaag aactaaaaga agatttagat agagctataa
     1021 aaaatgaaga gtatgaatta gcagctcaat ttagagataa aattaaatat ttaaaaggtt
     1081 ctattgacta agattggagt gatattatga aagagaacat agtcatgaaa tcaagagtta
     1141 ggctagctag aaatttaaat aactatccat ttccaaataa attagataaa gaatgtgcca
     1201 tggaaataat agaaaaagta aaaaatgcgt ttattaattc taacttagaa caaaaagaag
     1261 aatttgattt ctataaaatt gaagatttag accaatctaa aaaaatgctt atggttgaag
     1321 aacatataat aagcccagat ttagcagaaa atgataaaag tgcagttata gtaaaaaaag
     1381 ataaaactat aagcataatg ataaatgaag aagaccatat aaggatacaa actatatgtg
     1441 atgatttaaa cttagaatat gcatatagtg tagcaaatga aatagatgat ttgttagaat
     1501 caagtttaga atatgccttt aacacaaagc taggttatct aacatcttgt ccaactaata
     1561 caggaacagg aatgagagct tctgtaatga tgcatttacc agcattaagt caactaggct
     1621 atatggatga actttataaa atttcatcac aaataggtat agcaattaga gggatatatg
     1681 gagaacgaac agaagcattg ggaaatatat atcaaatatc taatcaactt acacttggaa
     1741 gaacagagtc taatattata gaaaatgtaa gtggacttac aaaagatgca atttcaaaag
     1801 aaataaaagc tagagaaata ttgcaaaaaa aattaggtat aaaactagaa gataaaatat
     1861 ttagatcgat agggacactt gaaaattcaa gagtaatgag tagcgcagag gcaatgagcc
     1921 atctatcaaa tataaaaatg ggaatagaaa tgaactatat agataaacta gatttaaggg
     1981 ctatagaaca acttatgata ggtatacaac cagcgcatca gtcaataatg tataagagtg
     2041 atgatgttga gaatagagat ataaatagag caacatatat tagagaaact ttggaaaaat
     2101 taagaggagg gatgaattgt gaacttcaat agatttacac aaagagctaa aaaggcaatt
     2161 gatttagcgt ttgaatctgc taaaagtcta ggacacaata ttgttggaag tgaacatata
     2221 cttttaggtc ttttaagaga agaagaaggt atagctgcta aagttctaag taaagttgga
     2281 tttacagaag cttatttaga aggcaaaata gttgatatgg aaggtaaggg agaagaaatc
     2341 tcagaagata tagtattaag tccaaggagt aaacaaatac ttgaattatc aggaatgttt
     2401 gcaaataaat taaaaacaaa ttatattggg actgagcata tattattagc aattattcaa
     2461 gaaggtgaag gaatagctaa taagatttta aattatgctg gagtaaatga taggacttta
     2521 gcccaattaa caattgatat gatgggtatt agtgataaaa atcaatacaa agcagagaat
     2581 agttacacgg gcaaccagaa tcaagcagaa tctaaagtac ttgataagta tggaagaaat
     2641 cttacactat atgcaaaaca gaataaaata gaccctgtaa taggtagaga aaaagaaata
     2701 caaagagtaa tacaaatatt aagtagaaga actaaaaaca atccagtgtt gataggagac
     2761 ccaggtgtag ggaaaacagc catagctgaa ggtctggcta caaatatagc cttaggaaat
     2821 gttccagaaa cactaaaaaa caaaacttta tactctttag agatgggttc attattggct
     2881 ggggctaagt atagaggaga atttgaagaa agaattaaag aagttgtaga tgaagttgtt
     2941 aaaaatggaa acataatctt atttatagat gagatgcaca caataatagg ggctgggtct
     3001 actggagaag gctctataga tgcttcaaat atattaaagc cagccttggc aagaggagaa
     3061 atacaagtta taggtgcaac tacaattgat gaatatagaa agcatgttga aaaagattct
     3121 gctctagaaa gaagattcca accagttatg gtagatgaac caactaaaga ggactcaata
     3181 aaaatattag aaggattaag agataaatat gaagctcatc ataaagttaa aataactgat
     3241 gatgctataa aggcagctgt agaattatca actagatata tatcagacag atatctgcca
     3301 gataaagcta tagacttgat tgatgaagct gcatcaaaag taagattaaa agaaaataca
     3361 cctccttcag aaataaaaaa attagaacta gaaatagaaa atatagataa agaaaaagaa
     3421 gaagcagtaa gatgtcaaga ctttgagaaa gcagccaaaa taagagatga acaaggtcta
     3481 cttaaaaaac aattagaaga tgttagagaa agatggaaca agtcatctaa acattcagat
     3541 ttagttgatg gagaagttat tgctgaagta gtgggcttat ggacaggaat accagttaat
     3601 aaaattcttg aagaagaggc tgatagactt ttgagacttg aggaaatact gcacaataga
     3661 gttataggtc aagaacaagc agtaaaatct atttcaaagg caatcagaag gtcaagagca
     3721 ggtcttaaag accctaatag accaattggt tcatttttat ttttaggtcc aacaggagta
     3781 ggtaaaacag aattatctaa ggcattagca gaagtgcaat ttggagatga aaatcaaata
     3841 attagaatag atatgtctga atatatggaa aaacatgctg tgtcaagaat gataggttca
     3901 cctccaggtt atgtaggtca tgatgaaggt ggtcaattaa ctgaaaaagt aagaagaaat
     3961 ccatattcag ttatcttatt tgatgagata gaaaaagctc atccagatgt atttaatatt
     4021 ctgttacaaa ttctagatga tggtagacta actgattcaa aaggaagaac tgtagatttt
     4081 aagaatacta ttgtaataat gacatcaaat gttggagcat ctacaattgg tagacaaaaa
     4141 actttaggat ttagtatagc taaaggagac gaagaagaaa aatctcaata tgaaaaaatg
     4201 aaagaaaata taatgggtga attaaaacaa agattcagac cagagttttt aaacagaata
     4261 gatgatataa ttgttttcca ctcactaaat gaaaatcata tatcaaaaat agtactttta
     4321 atggcagcga aactacaaga gagattaaaa gaaatggata taaaattaga aatgagtgat
     4381 gaggctgtta aattaatttc taaatctgga tttgacttag aatatggagc aagacctctt
     4441 aaaagagctt tacaaaaaga gttggaagat gagctatctg aagcaatctt aaaaggtaat
     4501 gtgaaaaagg gaagtaatgt agtagcaaaa gtcaaagatg aaaaaatagt ttttgaaact
     4561 aaataattta atagagttgc attttaaaat attgcaaata taagttttat aatctaaaca
     4621 aaattttaag ggcaatgaat ctataataat tcattgtcct taattatttt atttaatctt
     4681 tacatattgt ctttcagata tgctaaaatt atagtataga ataaaaaatt aaggatgata
     4741 aaagtatggc aaaaataaaa acaaaatatg tatgtcaatc ttgtggatat gaaacagcta
     4801 aatggcttgg taaatgtcca gaatgtacaa aatggaatac atttgtagaa gaaatagacc
     4861 aaaaaagcac caaaaaagag gtctttataa tagataaatc ttcatctaaa ccagtcagta
     4921 taaactcaat agaaagtaaa gaagaagaga ggtttacaac agatataaat gaattagata
     4981 gagtgcttgg aggaggtata gttaaaggct ctttagtgct tgttggtggt gaccctggta
     5041 taggtaaatc aacactttta atccaagtat ctagtaatgt tgccaattta ggaaaaactg
     5101 ttttatatat aactggagaa gaatcagaat ctcaaataaa aatgagagct aaaagattag
     5161 gtattaactc agaaaattta tatatatttg ctgaaaataa tttaagtata attgaatctt
     5221 atttagaaag tgtaaatcca gaattaataa ttctagactc aattcaaact gtcttcagtc
     5281 cagaaatatc ctcggcacca ggaactgtca gccaaattaa agaaggtact tcaaaattca
     5341 tgaaaatttc taaaaaaatg ggaatttcaa cgttcatagt tgggcatgtt acaaaagaag
     5401 gttcactagc aggaccaaaa ttattagagc atatggttga tactgttcta tattttgaag
     5461 gagaaagata taatacttat agattagtaa gagctgttaa aaatcgattt ggttcaacta
     5521 atgaacttgg agtatttgaa atgagggatt taggattagt agaacttgat aatccatcta
     5581 aaattttaat ctctgaaaaa ccaaaagatg tagcaggttc agtaataata tctactgtag
     5641 aaggtacgag accaatgtta cttgaattgc aagcattagt ttctcctaca agttttggta
     5701 taccaaaaag aacatctaca ggagtcgatt acaatagagt aggtatgtta ttagcagtat
     5761 tagaaaaacg tgttggtttg caaatacaaa atcaagatgt ctatataaac atagttggag
     5821 gaataaagat aaatgaaccc tcaattgatt tagggatagc catttctgta gcatctagtt
     5881 ttagaaatat accgatagat gaaaatatag ctgttacagg agaagtggga cttacaggag
     5941 aagttagagc agtaagcttt atagaaaaga gaatagctga gtgtaaaaaa cttggattta
     6001 aaaagatagt agttcctaga agtaattatg atgttgtaaa agagaccaaa ggcatagaaa
     6061 tatggccagt agataactta agacaagcta taaatatagt acttgggagg aatcaataaa
     6121 tatggagaat tttctagata ataaaaatat gctatatgca ttaaaaatga tatctcctgg
     6181 aactccactt agattaggtc taaacaatgt actaagagct aagactggtg gattaattgt
     6241 aattgcaaca aacgaagatg taatgaaaat agtagatgga ggatttgcta taaatgcaga
     6301 atattcacca tcatatctat atgaattagc taaaatggat ggagctatag ttttaagtgg
     6361 tgatgtaaag aaaatattat ttgctaatgc acaacttata cctgactatt ttatagaaac
     6421 atcagagaca ggaacaagac atagaacagc agaaagagta gcaaaacaaa ctggtgctat
     6481 agtcatagga atttcacaaa gaagaaatgt tataacagtt tatagaggaa atgagaagta
     6541 tgtagtcgaa gatatatcta agatatttac taaggcaaat caggctatac aaactctgga
     6601 aaaatataag acagtattgg accaagctgt aacaaattta aatgccttag agtttaatga
     6661 tttggtaact atttatgatg ttgcattagt catgcaaaag atggaaatgg taatgagagt
     6721 tacaagtata attgaaaaat atgtgataga attgggtgat gaaggaactt tagtaagtat
     6781 gcaattagaa gaattaatgg gtacaaccag aatagaccag aaattaatat tcaaagatta
     6841 taataaagaa aacacagaaa taaaagaact tatgaaaaag gtcaaaaatt taaattcaga
     6901 agaactaata gaattggtca atatggcaaa actattaggg tatagtggtt tttcagaaag
     6961 tatggatatg cctataaaaa caagaggtta taggattctt agcaaaatac atagactacc
     7021 aacagcaata atagaaaact tagtaaatta ttttgaaaac tttcaacaaa ttttagatgc
     7081 atctattgaa gaattagatg aggttgaagg aataggtgaa ataagagcaa catatataaa
     7141 aaatggactc ataaaaatga aacaattagt cttattagat agacacatat gatgaatttt
     7201 aaatgaattt tactactttt ttcttgaata taatatacac tttggtctat aatagataag
     7261 tataactagg gggtgaagca ttgataagga aagtaacaag aatattgttt attttacttg
     7321 gatttacaat aggtataaca acctatttaa cattaatgaa agattttgaa atattaacat
     7381 ttggaaaaga aacatatggt tatatcgcag ctgtagtagc aggtataata atagccattt
     7441 taggatatct tatagaacca tgggttgtaa ataaagtaaa agaaattgct aaaatagtgg
     7501 ataaagaatt gtcaaaatat cctcagactg atatattgtt agggtctatg ggtcttatag
     7561 taggatttgt tattgcgtat ttattaagtg gattagtaaa tagaatacca atagtaggtg
     7621 gaatactatc cttactatta tatttattcc taggatatct tggaatgaaa gttgcgctta
     7681 aaagtaaaaa tgatttattt aatattggaa aactaggaag attagcaaat cctattaagg
     7741 ataaagataa ggataaagaa aataaaaaag aagtaaaagc cattcctcca aaagtattag
     7801 atacaagtgt aataattgat ggaagaatag ctgatatttg taagactggg tttatagaag
     7861 gaaaacttat tattcctgct tttgttttgg aggaactaag acatattgca gattcttcag
     7921 atgatttaaa aagagtaaga ggtagaagag gtctggatat attaaatata attcagaaag
     7981 aattaaatat agaagttgaa ataagtgaga gagactttga tgatatagca gaagttgata
     8041 gtaaattatt aaagcttgct caggttttaa atggaaaagt tgttacaaat gattataact
     8101 taaataaagt tgctcaattc caaggtgttg aagtattaaa tataaatgag ttagctaatg
     8161 ctataaaacc agttgctata ccaggtgaag atatggttgt tcaagtggtt aaagaaggaa
     8221 aagaggctca acaaggtgtt gcatatctag atgatggaac tatgatagtt gttgatggag
     8281 gaagaaaata tatgaatgag actataaaag tactcgttac ttctgttctt caaactcctg
     8341 caggtagaat gatatttgca aaacctaaaa attaatatgt tcaaagaaac ctataatttg
     8401 ggctgtttat tttaaataga tggtccaaat ttatttattt ataacgataa tgtttaatgt
     8461 attcttaaag atggggattt aaatcagaaa gcagggagaa tttatgagaa aaaagattat
     8521 atctttctta gcaacattta tcataatatt aacatcagca agtcagtata gttttgcaga
     8581 tgatatgtca acacgtggaa aagtaatatt tatagatatg aatagaacta gtatgagtaa
     8641 tatgttaaga ataaaatcat taagagaaga actagacaat agagggtata ttggcttgat
     8701 gaatataaga ggcgataaag gaagtgatga tagaaggtca tatgctagta tgggtgctgg
     8761 tggtagagct aatgtggcta atgaagaaga tattaatttt gaaagctcga gtaaggatag
     8821 aaatatagtt tttgaatcag ctacaggaaa aagtgcaaaa ggtataaata atttaactat
     8881 aaataagtct ataaatgaaa atttaaactt tggagagtat ggttctgtat tagggtcatt
     8941 aggacaaagt ttatctgaga atggattaaa agcatctgta ttgggtaatt cagatataat
     9001 tgaaaatggt caacttataa aaaatagaaa cttatgtcta actgcaatgg atgaatatgg
     9061 aagaatacct aatggaaatg tagatactat aaataaaaaa gacttatcta tgccttatgg
     9121 aatatcaact gattatgata aattgattgt agagaccaaa gaagcttaca aaaataatga
     9181 tgtggttttt gtggagcttg gagacacata tagattagat ttatataaac caaatttaaa
     9241 tgaaaaaaca tatgagagtg tgaaagataa catagaaaaa aatatagatg tatatttaaa
     9301 aaatatattt tctatggttg aagaaaatga cactgtctac atagcaagtg catttccaag
     9361 tgatttagat tataaaaata aaagaagact ttctccaata ataaaattaa atggagaagg
     9421 gaaaggtata ttatcatctt caactactag aagagaaggt attgtaacaa atttagatgt
     9481 tggggttgaa atattagaca actttaatat aaaaaatcaa aatatggttg gaagaaaata
     9541 tgaacttatt aacagagatg ataataaaga atttttgatg gatgaatatc aaaagatagt
     9601 atctatttca agcataaggt ctacaatact gaatggtttt gtaagtatag ttttcttgtc
     9661 atggatagtt gctatgattg caattttatt tagaaagcat atatcaaaaa attataaaga
     9721 aacaacattt tttatattaa aggaactttt aaaaatagga atagtaatgc cactgtcatt
     9781 tatgataaca ccaataatga atttcaaaac accattagca atatctttag gaataattat
     9841 aataacactt actatatatc ttataagtaa agttttaata aagaatgatt taaaaaacat
     9901 gctgtttttt actggattaa ccatagtaat tatggttatt gatgctgggt ttggctcata
     9961 tttaatgaaa agcaatgtta tgagttatga ctgtataatt ggagctagat attatggagt
    10021 gggaaatgag tatcaaggtg tggcaatcgg tagtgcaata tttacatttg caattttact
    10081 tacatataaa aatataccaa agtggtctat aatagttttt tctttggtta tcttaattac
    10141 aagtgcatct ccaattatgg gagcaaatgt tggaagtgct atttctgaat gtgttgcatt
    10201 tttactattt atactattga tatataatgt gaagatagac ttcaaaaaaa ttgtattatt
    10261 aggtatagca gtattatttg tattaggagt ttttgtagca atagatatga ttttgggaag
    10321 taattcacat ttaggtatgt ttgtaaaaga aatttacttt aatggaccag gagaaataat
    10381 acaaacattt agtagaaaga tagagatgaa tttaaagcta gctcaaacca gtgcatgggt
    10441 aaatatactg ttaactggaa taggtgtaat tttagttctt atgattaatc agattagata
    10501 ctttaagcaa ctgatggatg agtatccaat tgtatttaaa ggatttatag catcaatagc
    10561 aggatgttta gtaactcttt tagtgaatga ctctgggatt gtgtcatcag ctacagcctt
    10621 tatttatgta attgtaccta tgataacatt aagtataaat ctcacagcat taaaagaata
    10681 agtactgtat gaataatatg taaatatgaa cacattaatt taacttcttt atttacaaaa
    10741 taaagaagaa agtttataaa aaataaatta taataaaaaa gagtttacct caaa
//
LOCUS       DAELSY010000137        10678 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.137, whole genome shotgun sequence.
ACCESSION   DAELSY010000137 DAELSY010000000
VERSION     DAELSY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10678
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.137"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..315
                     /locus_tag="KPG09_003022"
     CDS             <1..315
                     /locus_tag="KPG09_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0967610.1"
                     /translation="EDLQVKNMIKNRKLSRLISDVSWSEFVRQLEYKANWHGRQIVKV
                     GKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDINASINILKEGLRLITIQ
                     NK"
     gene            458..1006
                     /locus_tag="KPG09_003023"
     CDS             458..1006
                     /locus_tag="KPG09_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0967611.1"
                     /translation="MGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKADIEPVL
                     LKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEERLSEFI
                     NGNTLKYDFVKMPHHGRYDKLTETFLESTSPQYAVITCSEKKEPEEGVLKILERLNIK
                     TFLTSSGEVVINSDGKTLSVNQ"
     gene            1125..1316
                     /locus_tag="KPG09_003024"
     CDS             1125..1316
                     /locus_tag="KPG09_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967612.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            1539..1889
                     /locus_tag="KPG09_003025"
     CDS             1539..1889
                     /locus_tag="KPG09_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967613.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKTVTSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            2151..3656
                     /locus_tag="KPG09_003026"
     CDS             2151..3656
                     /locus_tag="KPG09_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBG0967614.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            4046..5143
                     /gene="dinB"
                     /locus_tag="KPG09_003027"
     CDS             4046..5143
                     /gene="dinB"
                     /locus_tag="KPG09_003027"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBG0967615.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGKPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKSSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            5399..6535
                     /locus_tag="KPG09_003028"
     CDS             5399..6535
                     /locus_tag="KPG09_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967616.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            6528..8075
                     /locus_tag="KPG09_003029"
     CDS             6528..8075
                     /locus_tag="KPG09_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG0967617.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            8409..9275
                     /locus_tag="KPG09_003030"
     CDS             8409..9275
                     /locus_tag="KPG09_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG0967618.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            9416..10558
                     /locus_tag="KPG09_003031"
     CDS             9416..10558
                     /locus_tag="KPG09_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG0967619.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
BASE COUNT         4138 a         1117 c         1917 g         3506 t
ORIGIN      
        1 gaagatttac aagtaaagaa tatgattaaa aatcgtaaac tatctcgctt aatttctgat
       61 gtgtcatggt cagaatttgt tcgtcaatta gaatacaagg ctaattggca tggtagacaa
      121 attgtaaaag taggcaaatt ctttgcaagt tcacaaatat gcaataaatg tgggtataaa
      181 aatgaggaag ttaagaactt aaatataaga gaatggattt gtcctagttg taatgaaact
      241 cacgatagag atataaatgc aagtataaat atattaaaag aaggattaag actaataaca
      301 attcaaaata aataacgtat gtaagaaccg taggaactac ggggatagcc tgtggagaat
      361 tagtaagaca tatgttagta tgcaaaattc tatgaagcag gaaccctgtg actttagtca
      421 taggaggttc agtttaaccc atttagataa ggaccatata ggtggagtag atagtgtgtt
      481 atctagtgtt aaagtaaaga atattataca agcaaattat aagaaagata gtaagcaata
      541 tgatgaatat attgactcac ttaaaaaagc tgatatagaa ccagtgctac taaaagataa
      601 tatgaatatt gtaatcaata gtgcagaaat aaacatacat ccggcttcaa aaagcaagta
      661 cgagagttca aatgattatt caataatcac taatataagc tatggagcac ataagttttt
      721 atttgcagga gatgcagagg aagaaagatt gtcagagttt ataaatggaa atactttaaa
      781 atatgatttt gttaaaatgc ctcatcatgg aagatatgac aaattaactg aaacattttt
      841 agagtctact tcaccccaat atgctgttat tacatgttca gagaaaaaag aaccagagga
      901 aggtgtttta aaaatattgg aaagattgaa tataaaaaca tttttgactt ctagtggaga
      961 ggtagttatc aatagtgatg gtaagacatt gagtgtgaat caataatttt ttttaaaata
     1021 tctaaaaaaa atttaaaaaa atcattagca aatattaccc cttaaacata gtatatttta
     1081 ggagctgaaa ctccacatac aaaatgacta ggaggtaaca tattatgttg gatagatttt
     1141 ttggtgaagg cggatttggt ggatgtgctt ggtggataat aatattattc ttcttattct
     1201 tagcattcca agattgttgg gctgaaatgg acttatgtgc atggatacct ttcttaatat
     1261 tattattaat actttgtgaa tgtggtgacg tttttgatgg aggatgtgga tgctaaaaaa
     1321 atcaatttag atgatttaat aaagcttatg gtgggtataa ataaaatacc aaaccaatca
     1381 caatctagtg tttgaaggta gtcggaaatc ccccgctacc ttcttttttt tattgttaga
     1441 atttattttt gaattatttc atttacatat aataattttt acaataaagt ctgatataat
     1501 taaaaagaaa ataaaattta tttataggag atgtaaaaat ggctatagtg gataagattt
     1561 tcaatgagat aaaacttatg gttgcaagac ttttattggg aaaaaaatac agtgaatatg
     1621 aagaaaaaga tttagtgtat aatacagaag aagaagttat acttataact ttaaaaagat
     1681 tagtttttca gggaaatgta aacgaagcag aagaaatttt atttgataaa gctaagactg
     1741 taactagtga aaatatgcag tatatagcaa tagaatttta tactatgctt atggaaaaga
     1801 ctgatgaaga attagaagct atgaattttt ctaaacaaga agtttatcaa ggtatagaag
     1861 atattagaaa agtattgaat ttagaataaa agatatatga aaatattgaa ataaattaat
     1921 gttaaggtga gaatattata cttatatata aaaatataat attcttattt tattagttat
     1981 attaggtata tcttatttac cataaaacta tcttgaaaaa aattaatctt aattgtataa
     2041 tattactttg agtgaaaaag tattaacatt tttttataag atgttttact aaaaaaatat
     2101 atataaatat actgttatca acaagaaatt taatttagtg aggtgttaag atgaaagtag
     2161 gatttgacca caaaaaatat ttagaagagc agtctaaata catcttagaa agagtaaata
     2221 attttgacaa actttattta gagtttggag gaaaattgat gggagacctt catgcaaaaa
     2281 gagtacttcc aggatttgat gaaaatgcaa agataaaagt attacaacat atgaaagaaa
     2341 aagttgaagt tgtaatttgt gtctatgcag gagatataga aagaaacaaa ataagaggag
     2401 actttggtat cacttatgat atggaagttt taagattaat agatgattta agaggttatg
     2461 aactagatgt aaatagtgta gtaattacta gatttgaagg acaacctgct acaactgtat
     2521 ttataaataa attagaacgt agaggaatta aagtttataa gcattatcca acaaagggat
     2581 atccgtcaga tgttgagact atagttagtg atgagggata tggagcaaat gaatatatag
     2641 aaactacaaa gccaatagta gtagtcacag caccaggacc taatagtgga aaacttggta
     2701 cttgtctaag tcagttgtat catgaaaata aaagaggtaa tgtagttgga tattctaagt
     2761 ttgagacatt cccagtttgg aatgtacctg taaaacatcc tttgaatata gcttatgaag
     2821 cagctacagt tgatttaaaa gatgtgaata tgatagattc attccacctt gaaacgtatg
     2881 gtgaaatgtc agtaaactac aatagagatt tggaattatt tccagtgctt aaaaaaataa
     2941 tagaaaagat aactggaaaa gaatcaattt tccaatctcc aactgatatg ggtgttaata
     3001 gagttggctt tggaataata gatgatgaag taataagaaa agcttctata gaagaaataa
     3061 taagaagata ctttaaaact gcctgtgagt ataaaaaggg acaagttgat aaaggcgctt
     3121 atgatagaat gaaaatgatt atggaagagc ttaacttaaa gccagaagat agaaatgttg
     3181 tattacctgc tagaaattac agtaataaat taaaagaaag tgccgacaag aatgatactt
     3241 gtcctgtagt tgcattagaa ttagaagatg gtacaatatt aactggtaag ggttctgatt
     3301 taatgaatgg aacagcagca gtagtattaa atgccattaa acatttagcc aatatatctg
     3361 atgatatgca cttaatatca cctgtaatat tagaacctat cataaactta aaaacaaaga
     3421 ctttagctag tagaaatgtt tcacttagtt gccaagaggt gttaactgca cttagtattt
     3481 gtgctgtaac taatccaact gcacagtttg ctatggaaaa gttagcatta ttaaaagggt
     3541 gtcaagctca ttctactact atattaaata gagatgatga acaaacattt agaaagcttg
     3601 gtatagatgt aacttgtgac ccagaatatc cttcacaaaa tctttactat agttaaaatt
     3661 atataaaaaa atatgggctg ttttaaagta atagtttgag cagtccatta caattataat
     3721 aaaatcagta ggctgtcttg tataagacag tcttttgttt ttaaaacatc ccataaatta
     3781 aatatatata ctgttatcca taaggaattt taaatttagt tccttggaat acctattgta
     3841 aagcttttat atagtttttg caagtgtttt taaggatttt ttataaatta tattgaagtt
     3901 aatggattat agatggtttt atttatggaa aatatgaata tataaagaaa ataatgaaaa
     3961 aataccaaaa agaaacataa gtttggtata attaaaaagt aatataaata aaattaatct
     4021 atttatactt taaaaggtga taataatgaa acattataga aaaataattc atatagatat
     4081 ggacgctttt tatgcatcta tagaacaaag agataataaa aaattaaaag gaaaacctgt
     4141 tattgtcggt ggaaatccac aaagccgagg tgtggttgca acgtgttctt atgaagctag
     4201 aaaatttggt atccattcag ctatgccatc agctgtagct tacaataggt gtccatatgc
     4261 agtttttgta agacctagat ttgatgttta taagagtgtg tcagagcaaa taagagatat
     4321 attttataga tacacggatt tagttgaacc attatcttta gatgaagctt acttagatgt
     4381 aacaaaaaat aaaaaaaata ttgattcatc aattgagata gcaaagcaaa taaaaaaaga
     4441 tatatttaga gaagttggtc ttacttcttc agctggtgtg tcatacaata agtttcttgc
     4501 taagattgca tcagacttaa gaaagccaaa tgggttaact gtaataaccg aagaaaatgc
     4561 ccaggacttt ttagataaat tacctgtgaa taaatttttt ggtgttggaa aagttacttc
     4621 aaatactctt aaaaatttag gtataaaaac tggatatgat ttaaggtgtt taaatttatt
     4681 tgaacttgaa aatatattca aaaaaagagg ttatgaatta tataaatttg ctagaggaat
     4741 agacgataga ccagtggaac caaatagggt tagaaaatct gttggggctg agactacatt
     4801 aagtcataat ttggatattg atgaagaaga aacacgcaac attcttgatg aactttgtga
     4861 agaagtatgt catagattaa aaagctctga gaagtttgga aaaacattaa cattaaaaat
     4921 taaatatgag gactttacaa agataactag aagccttagt ttagaacact atattgacga
     4981 atataatgat ataagaagtg gtgtagataa tttgttgaga aatgttgagg ttaatggaaa
     5041 acaaattagg ctattgggag ttacaatatc aaacttaagt gataaaaaag aaacttataa
     5101 agatattaca ttatttgaat acatggatag tattcaaatg tgagttttgt ttaaatgaaa
     5161 taacactagc tttaatacta tataattgag gctagtgtta ttttatgaat atttttatac
     5221 tttttgtttt ttgacattat atactatata taaatacttt atatttgtag caatattcag
     5281 acaaatagtg acctaaaaat cgtactttgt ttacataaat aaaatagtat gatataatta
     5341 taaaaacaac ttataatatt ggagaaggtt gcttgttcga aaaaggggat gataaattat
     5401 gatagagata agaaatgtaa caaaaaaaat aggaaataat gttatattag atgatatttc
     5461 tctagttgta gaaacaggta cattggtagt tttgattggt tcaagtggtt gtggaaagac
     5521 tactactttg aagcttataa acaaattaat aaaacctaca tctggtgaga tatatattaa
     5581 tggcaaacca atttctcagg aaaatgaaat agagcttaga agaaaaatag gatatgttat
     5641 acagaacaca ggcctattcc ctcatcttac aataaaagaa aatatagagc taattccacg
     5701 cctaaaaaag gaaaaatccg ttgaagaaat tgaaaaaaga acgttgcaac tacttgagat
     5761 ggtaggactt gatagtgatg aatttttgaa taagtatcct tcagaactta gtggtggaca
     5821 gcaacaaaga attggtgttg caagagccat tgctactgat gctgaaataa tattgatgga
     5881 tgagcctttt agtgctttag acccaataac gagaacatct ttacaagaac aattattttc
     5941 tcttcaagat gaattgaaaa aaactataat atttgtaacg catgatatgg atgaggcttt
     6001 aaagatagct gataaaattt gtattatgaa ggatggaaga atagcacaat atgatactcc
     6061 cgaaaatata cttagaaagc ctgcaaatga ctttgtaaaa gattttatcg gagaagatag
     6121 agtttgggat aatccagaat atataaaagc aagagatatt atgataaaaa atcctattgc
     6181 tgttaattcc actagaactg taactcaagg aatagaaatt atgagaacga gtaaggttga
     6241 tagcttacta attatagata gagcaaaaac attaaaaggt attgttactg taaaagatat
     6301 gaaagatatt gatgataagt caatattact tgctgatatt atgtcgagtg agcctttgca
     6361 tgtaaatgaa ggtgataatt tagtagaaat actaaatgta atgaatcgta attcggttgg
     6421 atatattccg gttatttctg atgaaaataa actggtaggg cttattacta gaagtagttt
     6481 actttctgtt ttaagtgaac aattcttaga aatggaggtg agtgttcttg gctaattttt
     6541 ttaactttat actattacaa aaagataaga ttatagagct tttaatacaa catatgagtc
     6601 taactgttac atctatttta atagctataa tagttggtgt tcctcttgga ataattattt
     6661 caagaatctc atctcttaga aagtttgtac ttggatttgt aaacttagtc caagcagttc
     6721 cgtctatggc acttttgggt cttcttgtac ctatacttgg cataggaagt aaaccagcca
     6781 tatttatggt agtggtttat tcattactac caattgtaaa aaatacgtat ataggtataa
     6841 caagtattga cccagtagta ttagaatcag ctaaaggtat tggtcttaca agaaatcaga
     6901 cattatttaa aatacaattc cctttagcac ttcctataat aatgggtgga gttagaatat
     6961 ctgcagttac agctgttggt ttgatgactc ttgcagcatt tataggtgct ggtggtttag
     7021 gatatttagt attttcaggg gttcaaactg taaataataa tatgatttta gcaggggcaa
     7081 tcccatcatg tataattgca ctgttagttg attatttatt tagtaaaatt gaagttgcag
     7141 ttactccaaa aggattaaat ccaaaagcac ctaagaaaaa ttatatagca cttaaagtaa
     7201 tatctgtagt ggttgttgtt tcaatgttgt tcgttgtttt tagttctagt ttttcaagta
     7261 aaaaagatac aataactatt ggttctaaag attatacaga acaacttata cttggtaatg
     7321 tttatgctga attagtagaa aaaaatacga atcttaaagt taagaagaat ttaaatctag
     7381 gtggttcatc tgttgcgttt aatgcaatta aatctggtga gttagacatg tatgttgact
     7441 atacaggtac acttttggta aatgtaatga aacatgctcc gattaaagat gctgatgaag
     7501 catataatgt tgtaaaagat actatggaaa aagaaaatca gttaacttta cttgaccctc
     7561 ttggatttaa taatacttac acattagcta tgatgcctga aactgctgaa aagtatggaa
     7621 taaatactat ttctgatttg actaaatatg gtaaagaatt tacattctca ccaactttgg
     7681 agtttgaaaa tagagaagat ggtcttgtag ggctttctag agattatggt ttaaaattca
     7741 aagatgtaaa agcaatgaat ggaagtttaa gatatacagc tttagataat aatgaatctc
     7801 aggttataga tgcattttta actgatggtc ttattaaaaa gtttaatctt aagatattag
     7861 aggacgataa aaatttcttt gtaccatatt atgcagcacc attagtaaga gaagatactt
     7921 tgaagaaata tcctgagtta gaaaaagtta tgaatatgct ttcaggaaaa ataaatgaag
     7981 aaactatgag agaacttaac tatcaagtgg atgaacttgg taaatcacca gaagaagttg
     8041 ctcattcatt cttagttaaa gaaggcttag tataaaaaat taatagttta tatcaaaaat
     8101 ttaagggagt gtctcaaaat gaacttttta gttcatgagg cactcttttt tatatgaaat
     8161 caaatatatt ttatcatttc aacaataaaa aagaagctta tctttatttt gagacagtct
     8221 cttattttta tgtataccaa aaccatacct acgattttta tttataatta agtttcaaat
     8281 agaatgaaca agtacaacca attttgaata taaatatagt aaatgtgctt atatacgatt
     8341 ttattatctg ggtgattgat attgatagat agctttcttt attgaatcga agggaggggg
     8401 aatttgttat ggataactat aagaaagtga agcaagtatt gtggataatt ttatttgcta
     8461 attttgcagt agcattatta aaaatcatta ttggtaatca aataaaaagc tatagtatga
     8521 cagcagacgg atttcattct ttgtcagatg gagcatctaa tatagtgggg cttataggta
     8581 ttttctttgc atctaaacca aaagataaaa atcatcctta tggacataaa aagtttgaaa
     8641 ttataaccag tttatttata agtggtatgt tgtttgttat agcaattaag ataattctta
     8701 gtgcagtgct aagaatagct aatccagttg tacctgctat aactatagaa agtttaattg
     8761 ctttaattat tacacttttt ataaatattt ttgtatgcat gtatgaatat agaattggga
     8821 ctaaactaaa tagttatgtt ttaatttcgg attcattgca tacaagaagt gatatatttg
     8881 tgtctctagg tgtattggtt actttagttg gtgttaagtt aggatttcca gtaataatag
     8941 aatctatagt tcctattatt atttctgcat tcataattta ttcggcttat ggaatattta
     9001 ggccatctat tggaattttg gtcgatagag tggctgtaga tgaggattat ataaaagaaa
     9061 ttgtgtttga atttaatgag gtaagggatg ttcacaatat cagaagtaga ggtagcaaaa
     9121 gttctattta tattgacatg catgtaatgg tagacccatt tataagcgta gagcaatctc
     9181 atgatttaac acataaaatt gagaaacaaa tacaagaaga aattaatgaa aatgctcaag
     9241 taatagtcca tattgaacct ttttatagtt tttaacttag ctataaaata caagttataa
     9301 aaaaatataa tttataaaaa aaatattata ccataaataa tttataaaaa ttatttatgg
     9361 tataatattt tctataaaga aattaagtct taatatatct aaggaggaat ttacaatgag
     9421 atacgttgaa tatggaaaga cagggaaaat ggtttcagta gttggctatg gaggattaag
     9481 gtttgattta gaaaaaagta atcaagaaaa tgctgattta gtcaaatatg cgtatgaaaa
     9541 aggtattaat tactttgata cagcgccagg gtattgtgat gacagaagtg aagatatatt
     9601 tggactagcc tttagacaaa tgataaagga aggtaagacc gatttttatg tatcaactaa
     9661 agaaaagcca aaagcatgtg atacatcaga aaaagctata gaggctgtaa aaaaatcaat
     9721 agaacgatta ggcgtatcta aaataaattt ttatcatgtt tggaatatta gaaagatgga
     9781 acattacgaa ctagcaatga gacctggagg tcaatatgaa ggattattga aatgtaaaga
     9841 agaaggattg atagaccata tagtattttc atctcatcaa ccaggagatg aggttataga
     9901 tattctaagt gaaaataagt ttgaaggtgt aactatggga ataaatatat taaactttcc
     9961 atataggcta aaaggtgcca aatatgctgt tgataatgga tatggtgttg tggcaatgaa
    10021 tccattaagt ggtggaacta ttccaaagta taataaagaa ctttcttttc tatcaaaaga
    10081 tggcgaaagt actatagaat cagctttaag attcaacata ggaatttcac aaataagtat
    10141 atctttgata gggtttaata aaaaacaaga tatagatgat gcatgtaaaa ttgcagacga
    10201 aaatagaatt tattcagatg aggatataca tgatattgag actagattaa ataagaatat
    10261 gaacgaaata tgtacaggtt gtggttattg taaagtatgt ccaaaaggta ttaatacccc
    10321 agcctatatg cttttttata atgaaaagca gatgtttaaa aagagtgatg aagaaatgac
    10381 aaagttagtt tatggtttag gacattggaa ttacacaatg aatagtaagg caaaagctaa
    10441 agaatgtata tcttgtggaa aatgtgaagt tgaatgtact cagcatttac ctataataga
    10501 tagattaaaa gaaattaaaa aatgggaaga agatggagct aatagtgtta aagtctaatt
    10561 aattttatac tatatttgaa atttgtatag tatttagtaa tgttttaaaa tatagtttta
    10621 atatttaagg agacagatta taattttgtc tccttaaatt atgctttaat ttgttata
//
LOCUS       DAELSY010000138        10329 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.138, whole genome shotgun sequence.
ACCESSION   DAELSY010000138 DAELSY010000000
VERSION     DAELSY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10329
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.138"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..188)
                     /locus_tag="KPG09_003032"
     CDS             complement(<1..188)
                     /locus_tag="KPG09_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0967620.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEG"
     gene            complement(458..1336)
                     /locus_tag="KPG09_003033"
     CDS             complement(458..1336)
                     /locus_tag="KPG09_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0967621.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            complement(1353..1670)
                     /gene="trxA"
                     /locus_tag="KPG09_003034"
     CDS             complement(1353..1670)
                     /gene="trxA"
                     /locus_tag="KPG09_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG0967622.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            complement(1820..3052)
                     /locus_tag="KPG09_003035"
     CDS             complement(1820..3052)
                     /locus_tag="KPG09_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0967623.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            complement(3042..3713)
                     /locus_tag="KPG09_003036"
     CDS             complement(3042..3713)
                     /locus_tag="KPG09_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0967624.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            complement(3729..4565)
                     /locus_tag="KPG09_003037"
     CDS             complement(3729..4565)
                     /locus_tag="KPG09_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967625.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            complement(4591..5265)
                     /locus_tag="KPG09_003038"
     CDS             complement(4591..5265)
                     /locus_tag="KPG09_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG0967626.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKRKQNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            complement(5279..6007)
                     /locus_tag="KPG09_003039"
     CDS             complement(5279..6007)
                     /locus_tag="KPG09_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG0967627.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIKDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSGLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            complement(6134..6823)
                     /locus_tag="KPG09_003040"
     CDS             complement(6134..6823)
                     /locus_tag="KPG09_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0967628.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLRVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMIGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(6831..7394)
                     /locus_tag="KPG09_003041"
     CDS             complement(6831..7394)
                     /locus_tag="KPG09_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0967629.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(7396..8364)
                     /locus_tag="KPG09_003042"
     CDS             complement(7396..8364)
                     /locus_tag="KPG09_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBG0967630.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(8465..8508)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            8873..9502
                     /locus_tag="KPG09_003043"
     CDS             8873..9502
                     /locus_tag="KPG09_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0967631.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKAIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQSY"
     gene            9477..9881
                     /locus_tag="KPG09_003044"
     CDS             9477..9881
                     /locus_tag="KPG09_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBG0967632.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            9884..>10329
                     /locus_tag="KPG09_003045"
     CDS             9884..>10329
                     /locus_tag="KPG09_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG0967633.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNKIELSK
                     FDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPSS
                     LLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNI"
BASE COUNT         3453 a         1712 c         1030 g         4134 t
ORIGIN      
        1 ccttctttat atcctgttat tatcccaaca tcttttaata tttttatatg ttgtgaaaca
       61 gcagaatctg aaatccctag atatttagaa atcccctttt gacatatatt tctcttggat
      121 actaatataa gtatgttaag tctagtttca tcactcaatg ctttaaatat tttaaccaag
      181 ttttccataa gtacctcgtt ttgattaagt aattacttaa ttaaattata gtactcttaa
      241 ttagattttt caaccattat ttttaaattt tggagtatat tataaataat tccaatttct
      301 actaatataa ctttgaattt tattaataaa agtaattaca tatagatttt aaaatacttt
      361 tattaataac ttaaaaataa aaaactgtct tgaaataata aaattatttc aagacagttt
      421 tttataaata tctttaaaaa gttaatcaat ataaatatta agccctcttt aatttatcta
      481 aatagtaaac agcatttaaa gctgctattt gcccttgacc agctgctttt atataagaat
      541 agggttttcc aacacaatct ccagcagcaa agcatccatc tatactagtc ttcatattat
      601 tgtcaacttt aatatgaatg ccatcaatct ctattccagg aaccaatgca cttggagccg
      661 tactatcttt tataacgaat actccatcaa cttcaatgtg gttttcttta aaagatactt
      721 tgtttacaag tttatctcca tctatctgta ctggtctgtc atgtataact tcaatagaat
      781 catctaggtt atctgaactt ctcataagat tatctttttt gtacataggt ataaaataag
      841 tctttgaagt gagttcactt aaaaaatttg cttcttcttt agattcttca ttatatccta
      901 taactgctac ctttttattt ctataaagtg gtgcatcaca tgtagcacag tagcctacac
      961 ctcttcctag aaattcttct tcacccttga ttggtctagt atactctacc ccagttgcaa
     1021 ggattacagt agtagcttca tacatatcat ttccagacat tattgtaaag tattctccca
     1081 tagcataaat attatttatt cttttatttt caattgaaat atccatacta tctatatgtg
     1141 atttaaactt gtcttttaat tcatcaccac ttatatcata aaaacctaaa taattatcta
     1201 ttgaaggagc tttaacaagc ttattactta aattatcatt accaaacatg ataattgttt
     1261 tatttcttat ttttgcattt atagcagcag acaatccagc tggaccactg ccgattatag
     1321 ctatatcata tctcataata ctcacccatt ttttataaat atgattttac ttgcatttca
     1381 attttgttct ttggcatgaa tccaattaaa gtttctactg gttttccatc tttaaatata
     1441 atcatagttg gtactgtaga tacattgaat tgttgagcta tttctaagct ctcatctata
     1501 tcaactttta caaatctagc tttatccttc atctcttcac ctaattctgc aaatacaggt
     1561 cccaacatgt tacaaggtcc acaccaagtt gcaaagaaat caactacaac tactccctta
     1621 ctaccttcaa cactacttct aaattcagat gtatttataa cttttgccat ttttaaatcc
     1681 tcctattaaa ctttattatc cctatataat tatactatga aatttataaa tataaattta
     1741 cttttataag gtctttttat attttatact aattagtata cccattcttc atttctaaaa
     1801 acaatttttt atatttttat tattttaact tttttggtat tgacaattca aatgtagttc
     1861 caatatctac atcagaagtt acatttatat caatagataa gtttaaacta atcatgcgag
     1921 ctattgaaag tcctattcca gaaccttcta aatctttatt tcttacattt tcacttctaa
     1981 aaaatctatc aaatataaga ggtatctctt ctttttttat gcctatacca gtgtcactta
     2041 taagtaaagt tacctcatcc tctatatctt cttttaattc taatgctatc tcatcaccca
     2101 attttgtata tttaaaggca ttatcaatga aaattaatat aagctgtctt aatttatttt
     2161 tatctgttat tataacttta ttcttcagtt tagaattaaa cacgaatctt ttttcctgaa
     2221 actcagcaat gtcaatatag tcttctgata tttcttccaa taatttttct aagtctattt
     2281 cttctaaatt aactttaaca atagagtcct ctttagtaag agacaataaa tcagttatca
     2341 tttttttaag tcttctagtt tccttcattg cagtagctat tgtttcaact tcatcattta
     2401 ctgtactttc tggggatttt aacatacttt ctaattttga tgaaactatt gttattggtg
     2461 ttcttaattc atgagaagca tcttgtataa acttagcttg atttttccat gctgtttcta
     2521 ttggaattaa agcttttcta gtgagatata cagccacaaa gtaagtaatt attacagaaa
     2581 ttaatattcc aattataaga acagatgtaa gctgtcttaa agaattcatc tcagaatcta
     2641 tatttctaat tatctgtatt tggtatctac ctacatctac atttaattct ctaaaagtat
     2701 atccattttc tgtgtattta aaaaacgaat tttttttatc tagtcttcta tctggaagta
     2761 actcatcaaa atattcattt tgagtatagt aacttatcct tccatcttca tatacataca
     2821 ccatatcttt aggatctttc agtcttattg ggttcaaaaa tgaagttctt ttaaattgag
     2881 agactatata ttcaagttca tcatttatct cactatcaat tccactatat gtaagtcccc
     2941 taaaatatga gtatataaat atagaaaaga ttattaaaaa actacctact acaataatat
     3001 acatttttat taaatgattt ttagtactgg taaatacatt cttatttatc atcgaatata
     3061 tatcctacct tacgtttagt tttaatatat ttatcatatc catatttact tagctttttt
     3121 cttagattac ttacatatac ttccactatt tcaatagttg catctgaatc aattccccat
     3181 attctatcat aaatctgctc ttttaataaa attgaacctt tatttaatac aaaatactca
     3241 agcaagttaa actgtttgtt ttgaagtttt atttcttctt tttcaatata cactcttttt
     3301 tctagtgtat ctaaatataa agatttaaat tctaatccgt ttctttcttt tatctttcca
     3361 ttagtcctta atatagcgta taccctagct actaactctt ccatataaaa aggttttgtc
     3421 aaataatcat tagctcctat agtaaatgct tctactttgt catctaatgt ttcttttgct
     3481 gtcaatataa gtacaggtgt atctatatta ttaactctca ttttctttag gatatcaaat
     3541 ccatttatat taggaagcat taagtctaaa ataactaaat catatatatt ttgatttact
     3601 aagtataaag cttcttctcc atcttcacac ttttcagttt caaaatgctt gctaagttct
     3661 tctactacac tttctaaaag tatcttatta tcttcaacta ttaaaacctt cataagtatc
     3721 ccctaccttt atttgtctat cttaacttta tgagatttta agtcaatatc cacattggct
     3781 ttttctttta tatcatcata cgtatatccc actatttcaa cactttttgc ctttgttgaa
     3841 cacaatatat ctatatctgt ctcttctgaa ggctttaaaa tcttatatga tggtacacga
     3901 tttactgtta aatattctcc attttcatta acttctgctg tcttaagaat aatatttcct
     3961 aaatcttttg atgatgaatt ttttactttt acttgatacg taattccctc atttgattca
     4021 tttacttttt taagttctga tgtagatagc acctcatact gactactatt ttctagctta
     4081 atattattat ttcttatatt aatagtattt tcttttaaat taacatacac aatcttaccc
     4141 tctgcctcat attcatatgc ataaacttct acacccttta caaaagtttt atgcccacat
     4201 tctatgtaag caactttccc aggcattaaa gtcatatcca acaaagcctt actatccgaa
     4261 gatataattt ttttattctc atcatattca ttatatatca attcaacatt ggatatatta
     4321 aatgttgaat tattttctac ctttgttgat gtatctactt tatcaccttc atctatattt
     4381 ataatttctg attttcctat attcaatact tttgcatttc cctcatctat ttttatctca
     4441 tttttatctt ctgtttgaat atcaactgaa tcaggctgtt catctttttc acatgctgtc
     4501 aacataaaaa taaatattgt taatataatc attagagaaa ttgatttttt tactatttta
     4561 ttcatattaa gcacctcata tagtagtatt ttatagtaga tttttatact ttttcataaa
     4621 ttctttcaat gcctgtgtaa gtatttgatg tttttcaaaa ttcgaattac tacataatct
     4681 ttcaaaatca ttccatattt ctttatctat acgtattctt gttgttttta attcatctat
     4741 gtgttcatca tctatatgta catctttttt attagatact tttttaggct ttatattgta
     4801 atcttttact tctaaatacc aatcatatac ttcacaaagc ttatctaaat cttcttgagt
     4861 tatatttatt tttctatctt tcttaggctt aacattttgt tttcgctttg cattttttaa
     4921 acctgtatca ttaaactcaa tttgttcaac acttttactt aaagcctttt cattagaaat
     4981 cttttttttg gtgcttactt tttttctatt acttatagta ttctttttac ttttttcata
     5041 ctccttagct ttctcttctt ccaagtttct attatccaaa cttttattat aatttaattc
     5101 gtcttttaat atgtatttac ctttttctga cttatatcct tttttattta aaaaagttct
     5161 taaagtactt tgactaatct ttagattttg agctatctct gcaaatttaa atcctttgct
     5221 ttgaagttct aaaaatttgt caattctgtc ttcattgtta ttcattaata ttcctccgtc
     5281 aacatcttct tgtctttctt ttcatacttc ttattatatt cttcttttct aaatctactc
     5341 tatttgattc aattattttt tgtagcattt tattataagt gaaatcatct aaattattat
     5401 tttttatata gagaaaagtt tcctcttcac atttaataaa acaaattgat agcaaccatg
     5461 ccatacccat tttaacataa tattcttcac tttgtatatt attacagtat tcaaatattt
     5521 ctttaatatg tttatcatca acataatata ttaaaaacat aactaatgca aatctaattt
     5581 cccatggatt ctttgaataa acatattttt taagtatatc atacatattt tctttatatt
     5641 ttttcataga ctttaaacca gaacaaaacc catcgcaaac tgaccaatta tcgattttag
     5701 gtataaatct ttttacatag tataaagttt cttcaaaatt actatttata ttgccaatta
     5761 ccataccttg tagcctaact tcttcatagt atgtatcctc tgctacttct aaaaattcct
     5821 tccaattccc ttggcttatt tcttttgata actttcttaa attaggaact ctaataccta
     5881 ttatattttc tgttccaggt ataagctttt tattaaaatc tctatatttt atatctttta
     5941 tagacatcat atattctaaa agttcattat aatccaattt attccatttt cccctatcaa
     6001 acatcattta attgctcctt aattcctttt ttcttattgt aacataaaac tctacaaaaa
     6061 aagcttctaa ctattagaag ctttttcaaa aacataagaa acttttcaat tttaaattta
     6121 aactaaatta atgctaatct cctacaccaa agtctgtatt attgtataaa acttctatat
     6181 ttggattttc atccaagaat ttatacttac taacaaacca tttcactaac tcttcatctc
     6241 tttttatact agaagaatta tttataaaaa cattcactgt cgctttttca aaagtattta
     6301 ggataatctc tatatcttta tctatcatct cttttgtttg tccttttatt ccaatcataa
     6361 tacaaggtga atcaaaatat tcttttacat cttgtggagt cttaaaattt gcatttttat
     6421 ttaaaatatt atttctaaaa tcattgtcaa aggtttcaac tccaatttta aataccacag
     6481 gaatttcaaa atactctctc atttccttaa gcctattttt gtaagaccaa tgactttcta
     6541 aaaataattt ttttatattc ttctctttta taatacactt tattttttct aaagtatcct
     6601 taggtaattc aaaacagctt ccagaattta tgacttcaag aactccatat ttcccagtta
     6661 cattttttaa aacccttaga tttaatttat tcatctcttc ttctaaatta gaattatcat
     6721 ctatataatc acaaaaagaa cactttcccc atatacaagg aaatccttta agcagaacaa
     6781 tttctctttg atttttattt gttatctcac tatatctatc cattttctat ctattttcct
     6841 ccagtcaatg atacctttaa agtttttgga actgcattta atcctaaatt cactaaaaca
     6901 actgctaaca gcttatctcc ataatcagta aatacttggg ttacaaacac actaaaaaca
     6961 tctcccagtc ctaacacttt caagatttgc actatatagg atgaacctga agaggtcata
     7021 cctccaaata aaaatgctga aattattgaa cttattatag atgttggaat agcaaataaa
     7081 agtactccta gtggagtttt taaacccctt aaaaaattct ttttaaatgc tattccagca
     7141 agtaatccgg ttgatatttg aactggtgca aaatagagag aatacacatc aaacgtaatt
     7201 ccacttatta aacttccaaa cacacctgta acaacagcgt atttaggtcc taaaagtgaa
     7261 gctatcatta tagttcctat tgaatctaag tatacaggta atcttaatcc catagctata
     7321 aaagcgccta ttatatttat agaaatacca aaagctataa gtgtaatagt ttttacacat
     7381 atttttcttt tcacactata tcactccttc cacagagtct ataatgtctt cataaccttt
     7441 aaaaagcctc tttaaaaaca tgtacataaa tttcttctca tcaacttcat ttagaacaat
     7501 tgcattagga ttttttttat agaaattgaa agaatccact atagactgac ccatagctat
     7561 tccatcttct acaacttcta catatgattc aaatccttta catatacttc tgtctataaa
     7621 gtacgctact gctagagggt catttatcac acagccaatt attccttcct gttcccaatg
     7681 gaaatcaata taaaatctag ttatttcagt tatatacttt gccatcttct tatcaagtct
     7741 atttataaac tcaataatat taggagtaag tacaattttt ctagttacat ctaaacctac
     7801 catgtggatt tttttagata aattcttgta aacataatct gctccatgtg ggtctaccca
     7861 ataattaaac tctgctactg gagagcaatt tccatgaatc ctaaatgctc ctcccataga
     7921 tacaaactca tcgagatttt caaatgcttt cttatcttta ataagtgctt tagctatatt
     7981 tgtaagtggt gcaagtgcta ttattgatac tttttcatga ttatgtaaag tatttattat
     8041 aaaatccact gctccatttt ttgctttagc tcctacaacc ttttgataaa agttttctcc
     8101 aataccatct tctccatgtg tatcttgagc tgttacaagt tttcttttta gtggtgcttc
     8161 ttctcctata tatactggaa tatttagtga agaacacatt tgaagtgttt ttagtgcatt
     8221 ttctgcacct atatttgctg gaacatttcc acaacatgtg gtaattccaa ttacttctag
     8281 ctctggtgag tttaaagcca gaagaattgc caaagaatca tcaattcctg ggtcacaatc
     8341 aattattact tttctctttt ccatttttta tcctccttga tttgtggtcg aggagaacta
     8401 cacatctttt ttccactttc aaaaaggtgt gtctaagggt attaattata tactgccact
     8461 atataattcc ttagtttttt atacttggag ttctcgaaca agtacagatg taaaatcata
     8521 tttttaggct acttactata gactcttaaa aactatagac tgttaaaaaa tttttaaaca
     8581 tctgatttaa tagctataaa ataaatttta tagcataaaa gaatttatta tattttaatg
     8641 tatcttatta aagtttattt agaattatga accaacttta ataagatgtg gcgcatttaa
     8701 accgattatt attatacaat aatatcttaa atattacaat taataacatt aatttattat
     8761 ccataaaaat atattaactt atattattta ataatttatt gacagtatta aaattaaatt
     8821 atacaataac agtgtaaatt aacttaacac cgttaagcaa agaggtgatt atatgagtat
     8881 caaagaaaga cgtaaaaatg aaaaagaaga aatgaagaaa aagattatgg atgctagcat
     8941 cgaaattata aaccaacatg gatatgaaaa tttatcaata agaaaaattg ctacaaaaat
     9001 agaatattct ccaactacaa tttatcttta ctacaaggac aaagcagaga ttatcagcga
     9061 tatgaccaat aaactttata acacagtaga aagtaatgct attgacatta tgaataattg
     9121 ctcatcacta ccaatagata aacaaatcat agaaattatg actagcttta taaaaactct
     9181 ttctagtgaa ccagagatgg caaaagctat catgcatagt agaatgaata ttatatttgc
     9241 atctgaaaat acaaacaata ctcctagcaa taatggtata aaaatgcttg ataaattcct
     9301 ttccattgga attgaacaag gaatattcaa aaaaaatata gataaaagct catggatgct
     9361 tataagtgca ttgttaggat tcgttttatg tgttgttgaa aataaactat attctttaca
     9421 taatttttct caacttatag aaaatttctt agatatttta gtgggaggac tttacaatga
     9481 aaattcgcaa caaagctact aataaaaaga aaaaatatgt aaaaatatct attgctctat
     9541 tctttgtatt tatatgcatt attattgtaa ttgtaagcaa cttaaagcca gaaaacttgg
     9601 tagtaaaaga tatagatatt aataatgtta aaaatggcat ttatactgga agtgctgata
     9661 ataacttggt aaaagctact gtgtctgttg aagttaataa tggtaaaatc caaaatatta
     9721 atatcttaaa acacgaccat cttttaggta aacctgctga aaaaattaca acaagtatta
     9781 ttaaacaaca gtctttagat gtggatgcta taacttctgc aacttatagc agtaatacta
     9841 tacgaaaagc tgtagaaaat gctttaagaa agggagaata attatgaata ctattattat
     9901 atattcaagt aaatatggtt gtacaaaaga ctgtgcaaat atactaaaaa ataaactatc
     9961 agataatgtc actattgttg atattaataa taataataaa attgagttat caaaatttga
    10021 taaaattatt attggaagtt ctatatatgt tggttcagtt tcaaaaaaaa tacaagtgct
    10081 atgtaatgat aatgtagagt tattaaacaa aaaacaagtt ggcattttcc tttgctgtgg
    10141 cttttctgaa caagcagata aatatttaaa atcaaatttt ccatcatcct tattagaaag
    10201 tgcaaatgct attggaattt ttggtagtga agctcgatta gaaaaaatga aatttttaga
    10261 taaacttatt atgaaggctg tttcaaaagg taattatgac agttttagaa tatcacagga
    10321 taatataga
//
LOCUS       DAELSY010000139        10264 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.139, whole genome shotgun sequence.
ACCESSION   DAELSY010000139 DAELSY010000000
VERSION     DAELSY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10264
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.139"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            48..782
                     /locus_tag="KPG09_003046"
     CDS             48..782
                     /locus_tag="KPG09_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0967634.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(924..1415)
                     /locus_tag="KPG09_003047"
     CDS             complement(924..1415)
                     /locus_tag="KPG09_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG0967635.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            1764..2147
                     /locus_tag="KPG09_003048"
     CDS             1764..2147
                     /locus_tag="KPG09_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967636.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKVVLKSPEYIGSHPKKPGKSVEFIKKIDNYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNNGRVKQYDG"
     gene            2653..3036
                     /locus_tag="KPG09_003049"
     CDS             2653..3036
                     /locus_tag="KPG09_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG0967637.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            3038..3388
                     /locus_tag="KPG09_003050"
     CDS             3038..3388
                     /locus_tag="KPG09_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967638.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(3482..3940)
                     /locus_tag="KPG09_003051"
     CDS             complement(3482..3940)
                     /locus_tag="KPG09_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967639.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(3985..4773)
                     /locus_tag="KPG09_003052"
     CDS             complement(3985..4773)
                     /locus_tag="KPG09_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0967640.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(4912..5349)
                     /locus_tag="KPG09_003053"
     CDS             complement(4912..5349)
                     /locus_tag="KPG09_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBG0967641.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKDCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            6171..6680
                     /locus_tag="KPG09_003054"
     CDS             6171..6680
                     /locus_tag="KPG09_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0967642.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDSLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVIVIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            complement(7591..8391)
                     /locus_tag="KPG09_003055"
     CDS             complement(7591..8391)
                     /locus_tag="KPG09_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0967643.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(8586..10184)
                     /locus_tag="KPG09_003056"
     CDS             complement(8586..10184)
                     /locus_tag="KPG09_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG0967644.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIIW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
BASE COUNT         3730 a         1486 c         1329 g         3719 t
ORIGIN      
        1 ttaatagagt gttgctaagt gaatatattt aaaatggagg aaactttatg aaacaaaata
       61 aatatgatga tgatagattt tttaataaat acagtaaaat ggaacgttct acaaatggat
      121 tagcaggtgc tggagaatgg catgttttaa aaaaaatgtt acctgatttc aaaggaaaaa
      181 gagttttaga tttaggatgt ggatttggat ggcattgtca atatgcagtt gagaatggag
      241 caatttctgc tgttggagtt gatatttcag aaaaaatgtt aaaagaagct agaaacaaaa
      301 cgcaacttga taatataaat tacatatgta tgcctattga agatattaat tttccaaaag
      361 atagttttga tgtagttatt agttccttgg catttcatta tattcaatca tttgaagata
      421 tatgtaagaa tataagtaat tgtctttcaa ataaaggtaa ttttgtattt tctgttgaac
      481 atcctatatt tactgcatat ggaagtcaag agtggtactg tgataaagat ggcaataatc
      541 tacactggcc agtagataga tattttgaag aaggagtacg taagtcaaat tttctaggag
      601 aagaggttat aaaatatcat aaaacattaa caacttattt aaatacgtta atgaaaacag
      661 gatttgaaat attagaaatt attgaacctc aaccagaaga aaaactgtta gatacaattc
      721 ctgatatgaa agatgagcta cgtagaccta tgatgttact tgtatctgca aaaaaaagat
      781 aagtaaatat aaataatcat tattgtagaa aaaaattgta gaaaataatt acactaatgg
      841 agatgattat accagtggta ttaattacac cagtagtaag gaggcaaaag cattcgttgc
      901 ttttgccatt ttttaagttg caattatact tctacgtagt atatttcgtt tccattttct
      961 tctataagct ttttcatatc ttcaaatttt aaaaatacag tagcagtatt atcatttgga
     1021 tgtataccta ttttatccat ttttaataaa tccttatcaa atacaacttt tacaacagcc
     1081 tctttatcat ttaatatacc aaaaggagtc acagctcctt tagtaagttt caagtatttt
     1141 tgaagtcttt cttctgatgc aaaactaaga ggtttactat caatttgttg acgaatatct
     1201 tttaaatctg cttttttgtc tttacctaat acaactaaaa aatgttcttt tcctttagca
     1261 tcacgaagaa ataggttttt tacaacaaat tccatatgta atgtttttaa ttcttccatt
     1321 tcttcaattg tataaactgg tggatgattt acagcctcat aaggtatgct taactcatct
     1381 aattttttat atacttcctg ttgttgattg ttcatgattt cctcccatta gaaatttatt
     1441 ttatatataa ataatattat cacagaaaac tttaaaagaa aacgttttat ataatataga
     1501 caatagatta tatttttata aagaagcaag aatactcaat aaaacttaaa gaaattaaac
     1561 aatttctaaa aataccttga aaatagaacc ttatttgcca tttataaatt atataaaaag
     1621 ggattatata aagtcatata tgaaaaaact atattatact atcttttctt tttggaataa
     1681 actatacttt aaatatagat atttaattta aataaatttt acagtattaa tagtaaatta
     1741 tcttagaaag gaatgtgctt tatatgtcta cagacaaagt tgttggaatc atagatgaag
     1801 aaacggcaga acttgcagga attgaatata ctggtaaaat ttatgcttct tctggtgtta
     1861 taaaacatat aaagaaaaaa catagatgtc aactgagtaa ggatattttt aatgatataa
     1921 tagacactat aaaagtggta ttaaagtcac ctgaatatat aggaagtcat cctaaaaaac
     1981 ctggaaaaag tgtggagttt ataaaaaaaa ttgataatta tattttagta gctacagaat
     2041 tagatacaaa aaaaggatat ttatatgtat cttcaatgta tgctataaaa gaagcaaaat
     2101 tagaaagtag agtaaacaat ggaagagtaa aacaatatga tggttaacat aatattatta
     2161 attttcccca aaatattgaa aaatattatt atataagtta taatattaat atacacgtag
     2221 atatttatta gttagatttg aggttggaaa aggcacccaa tacgctacta tagagtagtt
     2281 aatagagatg caggatacgc cgccctgcca aatctaatta aattaaaaag agaggattaa
     2341 ttcctctctt tttgtttttt tgagatacta atttaatcta tatagaaaga ttgaaggtca
     2401 tttatactaa aagttttcag ttatatacat taaactaatt aaataagata tttataatct
     2461 atatatgata catattatat attaataaaa gaaaaatata tagaaaatcc ttataaaaat
     2521 ttaaattgta atgaatagta agtaattaaa aagaattttt attgattttt aaaaatttag
     2581 ttagaaacta tatcattatg gtaaagaatg tattataatg tttagaaata ttataataag
     2641 gagcgattaa acatgaatct atcgaagaaa atagaagata aaagaaaagc taaacagaga
     2701 gctgaaaaag ttaagaaagc taaaattgct acagctggtg tggtattagg agctgttact
     2761 ggtgcagtga gtggagtact tttggctcct aaatctggaa aagaaacaag agaggatata
     2821 aaagatgctt cacaacaaat tgctgaaaag ataaatatga aaactgtaga tgtaaaaggc
     2881 aaagtatctg agaagctaga ggataaaaaa ggaaacttta tagaatcaaa gaaaaaaata
     2941 aaaaaatata tagataacaa aaagagtgtt gaaaattcta gtgaggaaga taaaataata
     3001 gatatagttg aaccagttag tgttactgag gaataatatg gacaatacaa taacgattaa
     3061 cataagtata atatatgtat tattagctat acttgcttgt attgctttag tttatttaat
     3121 tattgtactt gttaaactac ataagatttt aaataatgtt ggaactatgt taaatgaaaa
     3181 tactcaaaat ataaatacaa ctttaaataa tcttcctaat gttgtagaaa cttttggaga
     3241 agtaggagaa aatatgaaag atgtaacaga agtagttact gaagttacag cagattttct
     3301 tgttactaaa gacagtgtca aatcaaatat ggatgttata gtcgatatat tgactataat
     3361 taaaaatatt tttctagata aaaagtaata aatttaatat aaaataagat ataatttaaa
     3421 cgaccttaaa ataacattta taaggtcgtt ttttattagc ttgatttgac actatgtgtt
     3481 tttaataatt ggtaatattc ctctttatca tttgaacata tatatctatg cttgttgttt
     3541 caattacatc cataagaaca tctttacatt caagctcaaa atcttccatt ccttctaaat
     3601 attcatcatc ttcatcaaaa aagatataag aattatatag ccattcttca aatttttctt
     3661 caagtatatc ccaatacgaa ctccaaaata atccacaacc tgatatataa gatgctctac
     3721 tttgatttat ataaacctca tttagaagct tttgtaatct actgctataa agaaaatctg
     3781 ctcttccact gcttgatatt aattcattta aatcttctat tgacattaat tcaactttac
     3841 cacttttatc ataaggagta taattttcat caacccattt atgtggtttg ttttctctgc
     3901 atactgttgc tatcaactct acaacttttg ttcttatcat ttttttatcc taacttattt
     3961 attttttata aaataatcat gattttataa tttatctatt tcttgtaatg ctaatttaac
     4021 agtttcatat ctatcagcac catttattac tacatcacct tttacaagtt tattagcttc
     4081 tccacctaca acataaacct ttttacctaa accttgctta taactagacg cctcaattat
     4141 taaagaatct ttcaattgcc aatgtaaaat ctgagcagcc actttatcag catcacctgt
     4201 ataaacgatt acattttcat acactttatt ttcccctccc ccttgagaat ttgatataga
     4261 tttgtttagt ataccttctg ctatcaattt agcaacttta tctttattat taacataata
     4321 atctgtatct gctttactat ctacaaaaca tacttctatt aatattgctg gtgcaatagt
     4381 atgattaagc caataaagac ctctaacatc agactttgca cccctatctt tgaatactgt
     4441 agctaaccta gtattcactc tttctgcata ggttttgcca ttattagtct tatatattgt
     4501 ttctgtccct actggagttg aagttgtagc atttgaatta aaatgaatct gaacagccaa
     4561 atcagtttcc tgtttatttg ctatagcaca ttggtctgct aaatgattac ttgattcatc
     4621 aactcttcct gtataaacag tagctccacc tattttaagc catttaacaa ttaagtcatt
     4681 cagtattctt gtttctttgc tttcgtttat atacccaaca gctcctgtac cttttccaat
     4741 taaagtgtgc cctggtatta ttactacttt caaactaaca tctcctttac aattaaattt
     4801 aacttctatt taatatatgc ataacctaga attttgtttc tttttatatc ttatactttt
     4861 attataaccc attttgtaaa aatatagaat attaagtaaa taatgttaaa attataattt
     4921 tgtatataag acattacttg attgcaataa ttttatagca tattcatcta ttctatatcc
     4981 agttctataa tatatatgtt taattccagc ttgaattata gccttagtac aatttaagca
     5041 aggaaagtga gttacataaa tagagcaatc tttaactgaa ataccttctt ttgcacaata
     5101 ataaagggca ttttgttcag cgtgaatggt tctaatacaa tgtccatctc tcatttcatg
     5161 accaacttca ctacaatgct tatctccaga aatactacca ttatatccag tagacactat
     5221 tctgttttct gaattgacaa ttatagctcc tacataagct ctatcacaag ttcctctttc
     5281 agctactgtt tcgcacagtc tcataaaata ttcttgccat gagcatctat tttcaagttt
     5341 tttattcaaa tcatatacct ccaaaattta tatctaatat ataagattta taccttaaaa
     5401 ataatattaa aaacaaattt atacgataaa taaaatttat taatactgtt tttagtctaa
     5461 attatttcat tttaaattaa agttacaagg aatctaaaat tatatatatc tttcttcgta
     5521 atatgaatgt tacaatataa aaaactgtct caataaaata gagacagttt tacagtcaaa
     5581 gtttataata caagttcata tttttcttta aattctttta cagcttcggg ccatgccttt
     5641 tgttctgtaa tacctttttc tttagctatt ttaattgcaa tctctcttat cattgcagcg
     5701 ccttctaata ttcccatttt taatctccct tcaaatatct ttttgtatag aatataaata
     5761 gtgctaagat ttattacatg ctgcatatac aatatctata caacacataa cttgtaaatt
     5821 ccttttattt tcatacgtct ttaaccgaca tataattata ttcaataagt tttaaaatta
     5881 tgaactcaat tataattatt tttatttttt tagaatctaa acactaaaaa ttgtaggtaa
     5941 atttgtctta tgcctttata aatatttcta cagttttaat tactggacat atctattttt
     6001 attataaaaa gcgcttaaaa atcgagaata tccgcgtaaa agctgtatat aagcaaaacg
     6061 attaaaggaa aaagaagaaa tttacttgaa tgttaaaatg cgttgcttga agcaacgggc
     6121 agttttgcct ataatatcgg taacaactga aagaaaggtg gttagcccta atgttaagcg
     6181 aaaacattaa aacaatcagg aagtcaaaag gactttcaca acaagaactt gctatcaaac
     6241 tgaatgtagt gcgacaaaca atatctaaat gggaacaagg actgtcggtt cccgattctg
     6301 atatgttgat ttccatatca gaggtgcttg aaacgcctgt aagcacttta cttggagaaa
     6361 ctattgttga gtcggaagtt gatagcttaa aagtgatttc tgaaaaatta gagattataa
     6421 acttgcaact tgcacaaaga aaaaacatga gaagaaaaat gtttcattgg ttatttattt
     6481 tattatgtgc agtcatagta atagtttttg tggtcttagg gggactaaat agtccttact
     6541 tggattggaa ttataccgac cctgaaaccg ctgtttttgg agtagctttt catgcttttg
     6601 agtggctatt tgtcagatta gtaccgattg tccttatagg agcaattatt ggaatctttt
     6661 tgacacgtaa gaaaatatga agtaactctg ctttttataa tatagcaagc gttccttaaa
     6721 agtgtcattc tacatggcaa tcgaggacaa ctatgtaagg aagaaccttt ttgatttcca
     6781 actaagcgaa gttattgagg acgccagtag gcccaaaacc gcattaagta aagtacagga
     6841 agcaagactg ctggacttat actctcatgc gtcagatgca gaaaatgcga ggtctgtagc
     6901 atgatttaca acgatttgta caacgactac gagtagaaat atatggagat aagtattatc
     6961 tacgaaatca gaaaagaaat gacttgataa tttactcatg ttataattat tgatgtgaaa
     7021 ttaataaatc gaaattcctt ttttagattt aacaatactg agtaaggaaa aagtgaatga
     7081 cttttgcaag gaggtgttaa tatcaggcaa ataaagtctt ttgtttttcc agttatatcc
     7141 ttaatattct tagatcaaat tagcaaagtt cttataggat tattcttaat ggactttgaa
     7201 attgatataa ttgggaaatt tttaagattc aatcctgttc aaaatacaaa attaagtctc
     7261 tgctatctaa caaacggagc acaaaaatga aaattaaata ttaaatataa agcacagtta
     7321 attttgatta aaaagttgac tgtcttttct tttttataaa tttaagcatg ggtaataaag
     7381 aaatgactgc atttaagcat tatgtatata attggaggca ataaaaagcc agcaaagccc
     7441 ataaaataga gctgtgctag caacatgatg agttataaga agataatcaa aagcttacgc
     7501 tttattttaa tttaaaaaaa tgattactgt aaagaaatca ttttttatta taatcactga
     7561 tacatcaaat attctaaata aaatttttat ttatctggaa ttgtatattt tttctataat
     7621 attaactaca gcgtacattg cccaagcaca aatagccaat acaaatactc ccatcattac
     7681 taaatctaat ttaaatactt gagaaccata tactatcaag tagcctattc catatcttga
     7741 aactaaaaat tctccaacta ttacacctac ccatgccata ccaatattaa ttttagttaa
     7801 atttattaaa tttcctgtgt ttgcaggtaa tatgagttta aataatattt gagatttagt
     7861 agctccaaag ctttttaaca tcttaatttt ttcttcatct atattcataa aataattata
     7921 agctgaaagt atagtaacaa caacagaaat cgttacagct gttacgacga ttccttctat
     7981 tccagcacct acccaaacta taattatagg agctaaagca gtcttaggaa gagcatttaa
     8041 aactaccaaa aatgggtcta atatttttga taatttttct gaccaccaaa gagcaattgc
     8101 taccaaaata cctaatacag tacctataac aagaccaaga acagtttcat atacagatat
     8161 tccaatatgt ttaaataatt gaccactaaa tgcatattga ataaataaat tgtaaatatc
     8221 acttggtttg ctaaatagaa atgtatttat aacatttaag tcagctaaaa gttcccagat
     8281 tacaataaaa ccaattaaaa ttaatagctg gtagaataat acatttcttt tttctctttt
     8341 tacaccttta acatagttta tatatccttc agatctttta ttaagcctca tgtgtatcta
     8401 gctccttcca taatatgtcg aaatactttt gaaaatcagg aactttacgt gttttgagag
     8461 gcgtttttgt tccatccatg ttaagttcaa tatcgtaaat attttttatg gtagctggtc
     8521 ttttagatag tattgcaacc ttatctgata tgcttattgc ttctgctata gctaaaaaag
     8581 tgcaactaat tgcttaaaga aacagaatta tccacttcag cacccatata aacaatatta
     8641 agatttccta aactgctatc ccaaatgata ctattaataa tagtagataa taacaatctc
     8701 ttatcatcta aactggcatt atcaacttca tcattaaatc tcttcaaatt actaacaata
     8761 atatcaatat taatactgtc actactaata ttgatattac tagattccaa aatattaatc
     8821 ttatccttta aaatactaat ctcattattt aggctctcaa tctgggaaac tatataatca
     8881 gaagcagtac taccagtatt ttttgcaagc tctataacta aatttttaat aagtctttcc
     8941 ttatctaaaa tttgattttt aagtaaatta gtctcagatt taaaaatatt aaaaacatta
     9001 tcagtcttaa tgttattact attgaatgca cttataatag actcttcttt acaattctta
     9061 attttatcta taactaaact ttcaactaat ggaccattaa gatttttaca gttgcaacta
     9121 tcagacccaa gtgttttttt agtaccacaa atataataat actttaaatc cttgtcagtt
     9181 tttttgcctt tataggtaat tctcatatta gaaccacatt taccacattt tataagacca
     9241 gataaaagag cagtttttcc agttgatatc cttggagttt tatcacgatt tttatctaat
     9301 tgattttgta cttcaagcca tatttcaggt tctatgactc catggtgttt tgcaattgct
     9361 gctattgagt tagatgtatt tttagcatag gtaaggattc cacattttct atctggagta
     9421 cctacaacat ccataccact atttttaagg taatctaaaa tttcatcatt agcttttaca
     9481 tatgctggat ttctaagtat tctagataaa gaactagggt caaatttacc accacgagga
     9541 ccccttattc cattattata gacatattta tataacttag ataaggacct aagttctaaa
     9601 tatttattgt atattagctt tacaatctcc atttgttcat catttacttg aagcttatac
     9661 atacttcttt cttttagatt ttcatctaag aatgtgattt tttcactgtt gaatccatga
     9721 ggtgtattgc ctccaagcca tctaccacta cgggctaatt catacatgtt gtcttttata
     9781 cgttctgcta ttgtttctct ttctaattga gcaaatacac ttgatataaa catcatagct
     9841 cttcccattg gagttgaagt atcaaattgt tctttgatag atataaatga tatgtttagt
     9901 tcatttaatt tttctattaa agtagagaag tcagatacat ttctcgatat cctatctaat
     9961 ctataacaaa ctaggtaact aaatttctta tctttagcat cctgcatcat tttcttaaat
    10021 tgtggtctat ctatattgct accactaaat ccctcatctt catatatcaa aaaatctttt
    10081 acaccaatag actttgcata atcaatacat aattgtattt gattttctat agaatcacct
    10141 tttccagtaa atcttgattt tcttgaataa attgcagctt tcaaaattaa aacctcctat
    10201 aaattaggat atatatttga aattatttat atacaacctt ttaagtaaac ttattagaat
    10261 aaat
//
LOCUS       DAELSY010000140        10090 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.140, whole genome shotgun sequence.
ACCESSION   DAELSY010000140 DAELSY010000000
VERSION     DAELSY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.140"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            84..470
                     /locus_tag="KPG09_003057"
     CDS             84..470
                     /locus_tag="KPG09_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0967645.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            473..2272
                     /locus_tag="KPG09_003058"
     CDS             473..2272
                     /locus_tag="KPG09_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBG0967646.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVIILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSIIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLKQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(2627..4501)
                     /locus_tag="KPG09_003059"
     CDS             complement(2627..4501)
                     /locus_tag="KPG09_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0967647.1"
                     /translation="MNKYNKIANELIEIIGENNIISITHCATRLRVMVKDREIINDKK
                     VEKVNEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEVEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIGLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(4506..6170)
                     /locus_tag="KPG09_003060"
     CDS             complement(4506..6170)
                     /locus_tag="KPG09_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBG0967648.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDATPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSKYSDCLIYGSIEPIDIESDNVIAYRRQLDLVVIDCY
                     YNFSDKLVEIKTEEKYDIVFQNLDDINLLNKTVSLKPFQAVLLKVR"
     gene            complement(6184..7005)
                     /locus_tag="KPG09_003061"
     CDS             complement(6184..7005)
                     /locus_tag="KPG09_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0967649.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSIGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDVHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(7104..7868)
                     /locus_tag="KPG09_003062"
     CDS             complement(7104..7868)
                     /locus_tag="KPG09_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0967650.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVVKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDTSTIIDFAKEMRMKGTYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(7865..8614)
                     /locus_tag="KPG09_003063"
     CDS             complement(7865..8614)
                     /locus_tag="KPG09_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0967651.1"
                     /translation="MRLESLVNQSYNSLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKSGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(9118..9723)
                     /locus_tag="KPG09_003064"
     CDS             complement(9118..9723)
                     /locus_tag="KPG09_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0967652.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMKIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            9902..10000
                     /locus_tag="KPG09_003065"
     CDS             9902..10000
                     /locus_tag="KPG09_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBG0967653.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFD"
BASE COUNT         3617 a         1641 c         1116 g         3716 t
ORIGIN      
        1 atgaattatt tttaataatt gacaaactaa attaatgata atatacttta tgtattacaa
       61 atgtaagtag aaggaggtat agtatgaaaa agttaccaca tatttcagaa gcagaatatc
      121 aagttatgaa aattatttgg aaatatgctc ctatcagcac tactgaagta attgaaaagt
      181 tggtagaaac aagtacatgg agtcctaaaa caattcaaac aatgttattg agattagtta
      241 aaaaaggagc tttaacatat gaaaaaaata gcagagtatt cgtttatact ccacttgtta
      301 aagaagaaga atacgtagct acagaaagta gttctttttt aaatcgtttt tataatggta
      361 ctcttaattc tatggtacta aactttttag aaaatgataa actatctgaa gatgatattg
      421 aggaactgag agaaatctta aataaaagga cgactaaggg ggataaataa atatgtatgc
      481 cccattcttt atacgatttt tactcagtac attaacctta tcaatattaa ttgttataat
      541 tttacttgcc aaaaaagtat ttaaaaaaca tatttctgca aagtaccaat acaatattgg
      601 atttatattg cttataatgt taataattcc atttgtccct cttaaatata ttaatcttgg
      661 aaatgtattt aactatttat atacttttaa tcaacttgga agcagtacta ataggacttc
      721 tattataaaa aatagtccaa attctataat taataactca aatgcattaa acgatttttc
      781 agtatcccta aaccaagtta attttgatag tttaaatatt ttattactaa tatcatggat
      841 aattggaatt ttaatcatga tagctattac tattcattgc aatattaaaa taaaaaatat
      901 gaaaaaatca atacaaattt taagagatga ggagatagta tttatatttg aaaagtgtaa
      961 aaaagatatt ggtataaata aggattttat actatgcaaa tcacctatta taaatacacc
     1021 tattacattt ggatacttta aaccacacat tatattacca gataagtcta tatctaaatt
     1081 atctttaaaa gatataaaat atatcttact tcatgaatta caacacttta aaaataaaga
     1141 tatactgata aattatataa tgtgcttcct tcaaatatta tattggttta atcctcttgt
     1201 atggtatgca tttaaagaaa tgcgtataga ccgtgaaatt gcatgtgata tttcagtttt
     1261 aaaaaagctt gataaagata gtcatataga atatggacaa actctaataa actttgtgga
     1321 taatatttca agaccatcaa atcttacact tacatcagat attggtgggt caaaaaaaca
     1381 aataaaaaag cgactattga agataatgga ttttcataat gaatcaaata cgttaaaatt
     1441 caaaagcatt ttaattttta cattgatagc tttaattgta tttggaaata ttcccaaact
     1501 atctattatg gcagatatca acgaaaaata tagacctaca aatgaacaaa ctgtgtacga
     1561 ggacttaagt cagtatttta aaggttttga tggaagtttt gttatgtatg atttaaaatc
     1621 caagcaatat catatttaca ataaagaaaa aagtaaaaca agaatttctc caaattctac
     1681 ttataagatt tatagtgcct tattgggatt ggaaaatggg ataataacaa gaaataattc
     1741 acatatccct tgggatggaa agcatcattc taatagttct tggaatgaag accaagactt
     1801 attttctgct atggaaaact ccgtaaactg gtactttcag actctagaca aaaaaacagg
     1861 gctaaaaaac atacaaactt atcttaagca aattggatat ggcaattatg atgtttcagg
     1921 tggtatctca aacttttggc tagaatcttc attaaaaata tcacctattg aacaggtaga
     1981 attacttgaa agtttttata caaataagtt taaatttgaa gataaaaata tagaaacagt
     2041 aaaagattca ttgttaatat caaaaaagga tggggtatca ctttttggta aaacaggcac
     2101 tgctaatata aatggaaaag atgtaaatgg ttggtttata ggttatgtgg aaaaaaatga
     2161 aaatacttat ttctttgcta caaatattca aagcaaagat tattctaatg gtagttcagc
     2221 atctaagata acattatcca tattaaatga caaaaaaatc tataatcctt aatatattta
     2281 ctgtacattc taaaaaattc acataatttt catattaatt atcgattaaa ttcatataca
     2341 aggcttatta tataaacata gaagatagta aaactagctt aaaaacataa tataataccc
     2401 tatacaaata gtggcaactc tccctcacat cctcaattgt tgccactatt taattttttg
     2461 tatgaagtta aatatatttt catcatttca acaatttggg tgcagtacaa tctctatttt
     2521 gagacaacct ctttaattgt tttagagttt atgaattatt tctcatactt taaaaagatt
     2581 gaattactct tgataatctt cttttgtagt cattacatat cacctactac tcaacacata
     2641 ttctttgacc tatttttaca tccttgttta tatccaaaat atttaccttc ttaccatctg
     2701 gaaacaatac cattgtcaaa gtagactttc ctaattcatg tattctcttt aaatcaacat
     2761 taacaagaac atctccctgt ttaaccatat ctccttgttt aacagcaata tcaaagccat
     2821 ctccatttaa tgcaactgta tcaattccaa catgaattaa tacttctcca ttagttcctt
     2881 taatcccaaa tgcatgtcca cttgaaaata ctgtttctaa tttacctgaa ataggagaaa
     2941 caatttttcc atctataata tcaatagcaa aaccatcacc taacattttt ttagaaaatg
     3001 tttcatctgg tacattttct aatgcaacaa ttttcccctt agctggagac acaaagtttt
     3061 cattattttc attaaacact aattcttcgt cttcatcttc atttatagct tctttaggca
     3121 ctccaaataa acatgttaca ataaagcaga atgcaaccac aaatattgat actaataagt
     3181 atgtgattaa attatatcca tcatagatat acattaaata agaaggtaaa actgctaatc
     3241 catatgaatt agattgaata tgtaaaattg ataatattgc tgctcctaaa cctgaagatg
     3301 cacacataat aatcaaaggc tttaaattat atcgaagctg aatcccaaat aaaacaggct
     3361 cacttatacc aaaaagttga gatagcattg ctccaactgc agtttgttta acttgattat
     3421 ttttagattt tttcataaat gctagacatg ttccaatatt cgcaaatcca tacattgcac
     3481 aaagagtaat aagtgcatta aatcctgtat ttgctaatag tgatgtttca atcattacat
     3541 aagtatgatg taagccagta ataacagcta atggatatgt aaatccaata ataaatcctc
     3601 caattcctaa aggtaaacca atcaataaac tgatgatatc aaccattttt aattctattg
     3661 tatgcattat aggccctaca cctaacaaaa taactaacat tgttattagc ataacaaaga
     3721 atggtgtgaa aataaggtct aatacattag gcatatgttt acggaaccat ttttcaagat
     3781 tagctccaat aaatgctgta acaatagctg ttaaaacact tccttgacag cctacaatag
     3841 gaataaatcc aaatgccata attgcttcaa cttcatttga tgggtcagca acagaatatg
     3901 cattaggaag tattggagaa actaacataa gtccaataac aagtccaata acaggtgttc
     3961 ctccaaatac cttaaatgct gaccatacaa taattgctgg taaaaaagca aatgctgttt
     4021 ctgtaagaac cgaaacaagt gtctgaatat acaatggaat atttgcagaa gacattccaa
     4081 ataatccaag tacattatca ttaaacaaac aaccctttaa tcccaagaat aatcctgttg
     4141 ccgcaataac tggaataata ggaacaaata tatctgctag agttctcata actttcttaa
     4201 atccagtttc attattttta actaattcat cttgttcttt ttttgataaa gttttcaatc
     4261 ccattttttc aacttctgca taaactttat tgactattcc cgttcctaga ataatttgat
     4321 actgtccaga agtaaagaaa acacctttaa cctcattaac cttttccact ttcttatcat
     4381 taataatttc tctatcttta accatcaccc ttaatcttgt ggcacaatgt gtaatagaaa
     4441 taatattatt ttcaccaata atttcaataa gctcatttgc tattttatta tatttattca
     4501 tttttctacc tcactttcaa taatactgct tgaaatggtt tcaaactcac tgttttattt
     4561 agtagattta tatcatctaa attttggaat acaatatcat atttttcttc tgtttttatt
     4621 tctacaagtt tatcagaaaa attataataa caatcaataa ctaccaaatc taattgcctt
     4681 ctatacgcaa taacattgtc actttctata tcaataggtt ctattgaacc ataaatcaaa
     4741 caatcagaat acttactatc ttgtctcaac tttatcatct ttttataaaa ttctaaaata
     4801 ctttctgaat tgttaatctg actctgtgca ttaatatctt tataattttc aataaccttt
     4861 aaccatggtg ttgcatctga aaaacctgca ttttctttat ctgcccatgg aaatggcgtt
     4921 cttgcattat ctcggcttct taaattcaca aattctaacg cttcttcttc tgagtaacct
     4981 tcatctattg aacgatagta ttggtcaatg ctagataaat cattaaaatc atttatggta
     5041 tctcttttaa aatttgtcat tcctaattcc tgtccctgat aaataaatgg tgttcctctt
     5101 aaaaagaaat acattgctcc caacattttt actgcatcat cattcctttc gtattgcaaa
     5161 agatactttg ttgtagctct tggttggtca tgattttcaa taaaattagc tgcccatcca
     5221 tgttcctgaa catacatttg gctatctaaa atgcattttc ttaaatctgg aattgtccat
     5281 gaaattcttt taaaccactc actaccagaa gcaacatcta agtcagcatg tttaaagtca
     5341 aagaccattg aaaagaaacc atcttctcca ataaaatcac ctagttggtc atagctaata
     5401 cctgctgctt cagcaacagt catacaatca tattttttaa acgtattttc ttttaattca
     5461 taaagcattt tttctattcc aggctgattt ctaactgttt ttgtacattt aacaagacca
     5521 tcaacaccat cactttctaa actttgaaaa cttaaattct ttttgataaa tgtaatagca
     5581 tcaactctaa atcctgccac tcccatttga gaccaggtat taatcatttg gtaaagttca
     5641 cttcttaata ttggattttc ccaattaagg tcaggttgtt ttttaccaaa agaatgaaaa
     5701 taatattcgt ctctattctc taccttttcc caaacacttc caccaaaaac agacctccag
     5761 ttatttggta aatcgtttcc ttttttaaaa atataataat catgataagg actttctgga
     5821 ttttttaatg cttcttgaaa ccaaggatgt tcatcacttg tatgattaag aactaaatct
     5881 aatataattt tgattcctct atctttactt tctttaatta actcttttaa atcttccata
     5941 cttccaaatt cacttgcaat atctaaataa tcactaatat catacccatt atcgtccata
     6001 ggtgatttaa aaattggaca taaccacaat gatgtaatac ctaaatcttt taaataatct
     6061 aaacgagaaa taattccctg taaatctcca attccatcat tattactatc ttgaaagctt
     6121 cttggataaa tttgataaac gatttctttt tgccaccatt tcttttccat aaattactcc
     6181 tttttatatg atatttcttc tttttaattc tttataaatt ccattatcga atatgtcctc
     6241 acatatagat gtcgctatat ctttaagttg ttgatgacta tttttcattg caattgtaac
     6301 gtctgatgct tgaaacatga caatatcatt ttgaccatca ccaaaacata tggtttcttt
     6361 ttgagttacc cctaatcttt cttgtaatct cttaattgct ttgcccttat gaaaatcttt
     6421 ttgaataatt tcataatact gactagaaat atctctctgt gctaattcca ttttatcttg
     6481 tagtatatct ttcacttcgt caaatacctt ttcatttgac cataaacaaa ttttatgtac
     6541 atcttgtgac ttatactctt caatattatt ttcatatgtt attttttctt ggatatgttg
     6601 tttatttata caagaattag ttcctttcaa ctgattcatt gtttcaaata tttctttggc
     6661 tttttgattc atgaaaactt tttcctgact ctcaatcgaa aaagcaactt ctctcttttt
     6721 taacaaacaa acaacttcct ttatcaatct ttgattaaaa gattgattat aaagtaattc
     6781 accatgatat tgaatgtaat tacctcctcc tgcaatatat ccatcaaccc ctaatgataa
     6841 aacatcatct tgtattgtac ctatacttct tccagtacaa ataacaacac tgcaatgatt
     6901 cttttgacat aaacgtatag cgtgctttgc agattctggg attccatata cctcatccct
     6961 aagcgtacca tctatatcaa gtattaataa tttgtacaat ttcatcttcc tttcttacta
     7021 tcaaaatcat aatagcattc aaaaaaaaag atatcaatat tttgatatct tttaaaaaat
     7081 atgttcattt ttagcacaaa ttttcacttt cggggaatat gttcactttc taaatacatc
     7141 ttgtatttaa tataaagcag ttcaattaaa ataaaataag gtgttgttac atcgtagtcc
     7201 agttgtttat ctacagaaat agatatagat tgaatataca ggttttcatc acataatctt
     7261 gctaaaggat tacttatcat ttttgtaata gaaaccatat aagttccttt cattctcatt
     7321 tctttagcaa aatcaataat tgtagatgta tctccattca atgagataaa gaaaatcata
     7381 tcatctgttt ttataaaatg attaagcgcc tgaatcatat cataaccata aacatcatat
     7441 ataattttcc ctgttggtaa gaaaatcctt ttcatctctt taacaactct ttcctgagaa
     7501 taaccttctc caaagacgat tatacgattt gcctttttca ttctttcaaa cataatcgaa
     7561 caatccttct tctctatatc atctatcacc ttattataac aatccattaa ttggtgttta
     7621 ctttgatttt tatattctac ttctgtttca tttaagcgta taataactct taattcacta
     7681 tatccaggta attgtagttt ttgagcaaaa cgagaaagtg cacttgtaga tatatggtat
     7741 ttgtctgcaa attcatctat agaaagttta atacaatctt ctttatgatt aagaatacac
     7801 tcacagatat acttatcatt tgctgtaaat gaattataat atttattgaa aagtccttca
     7861 attttcattc ttttctcctt ctatactcta tgtaattcat atgaagcaaa tctaatagag
     7921 cataaaatgc tcctacgact tcataagata tagcttgtaa tgtatcaagt ttttgagttt
     7981 caacatacag attatactca caaagtgttg caattgtatt attatcaagc ctagttaaag
     8041 aaatcgtatg tatatgattg ttgataaatt ttgcaatttc aattcctgat tttgtttctc
     8101 cagacaaaga aatgataata aataaatcac ttttttgaaa tgattctctc ataaattcaa
     8161 tttcacctat atcaaataag tcaataacac atttacctac tgaaagaaat attctcttta
     8221 tttcttgagc taaacttttc tgttcattac ctgtcccata aatataaatt gtttctgcat
     8281 catatattat tttacaaata tcttgaaatg atattttttg taattcattc attaatagtc
     8341 gataattctc gcaaacatca tgaaaattat tatcattatt aacaaattct agttgattat
     8401 ttttaagtaa atactttaaa tcagagaatg aatctaatcc aatttttcta caaagcctta
     8461 ataaagtagc tctagaaaca ttacattcat ttgcaatttt ttcacacgaa aaattacggt
     8521 attctctttg acaaatcaat atttgtttaa taataatttt atcattttca tttaaactat
     8581 tataactttg gttaactaac gattctaatc tcattttttc cactccctaa atattatttt
     8641 aatatacatt agttgctttt tctagtgaag agtcttaaat aataaggata aaataatcat
     8701 ttgattttat ttttattaaa ataaaaataa cctgctaaaa gcaggcacca aaaagtactt
     8761 atcctttcga acatgtactt tagatattgc tttcttcgga agcttcatct cttcatactc
     8821 taaaatagag ttttttctaa ctcaaaagta ttttccatgg tagaaattac tttaattcat
     8881 ctgccattta ttttttatct tatttaataa ttactattac ttgaatattt acttctttaa
     8941 gtaaatttca tagttaacaa agctaaaact attattatag atgttaaaat acaaaattat
     9001 aagtattgtt ataaatatgc ttacagctac aaatatacat gatatattaa aaagaagctg
     9061 taaattatta tcatacatac aagcaactat gcctctatta atccatgtta atttttatta
     9121 tttaatacaa ggagttccat gagacataca tatccattca tattttaaat tatccaattc
     9181 tcttatagat ttcatcatct ttttataatc ccaattccat ggattaggat aaggaactat
     9241 atttccattc ttatttttga ctaaatcacc tgcaaataat acatcttcaa aaatcatgca
     9301 gacatgccct ggagtatgac caggagtatg gataattttt atattcccaa tcttcatatt
     9361 ctctccataa ggttcaacat ccttaatgag ccttctggaa attgcctttc caataaactt
     9421 cttaaaccca ggtctatcct tctctcctat aataaaaggt atatcctcta catgagccca
     9481 tacctttgct cctgttttct ctttcaataa tttaatatta gatatatgat caacatcatg
     9541 atgagttaat aaaatatgct taatatctgt aagtttaatg ccaagtgttt ttaattcttt
     9601 aattatattt ttccccataa aaggaagacc tgtgtctata agaataattt ctttatcgaa
     9661 cacaataaaa caatttgaac cacgagtact atctaacatt aatacatttt cttttatctt
     9721 cataaataaa ttccccttat ttggtatttt acctatataa ttaattaata aagttataaa
     9781 tagttataat acttgatatt aattttattt tttattaaag tatactatag gtatacaaat
     9841 attatattta cataagtata ccatcagtat acttttttat caatagaaag gaataattta
     9901 tatgtcagaa ttatcaaaaa gagataaaga aaaaatacaa agagaaaatg agattattga
     9961 taaggcagaa aaattatttt gtcttaatgg atttgattaa aaaatagcta cgcacactcg
    10021 tgactttagt catgagttag tagctaaaat agtcagcata tagggaaacc tatatgtaga
    10081 gataggataa
//
LOCUS       DAELSY010000141         9826 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.141, whole genome shotgun sequence.
ACCESSION   DAELSY010000141 DAELSY010000000
VERSION     DAELSY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9826
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.141"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    212..423
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            586..1875
                     /locus_tag="KPG09_003066"
     CDS             586..1875
                     /locus_tag="KPG09_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG0967654.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1929..3008
                     /locus_tag="KPG09_003067"
     CDS             1929..3008
                     /locus_tag="KPG09_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBG0967655.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            3029..4573
                     /locus_tag="KPG09_003068"
     CDS             3029..4573
                     /locus_tag="KPG09_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBG0967656.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4736..5278)
                     /locus_tag="KPG09_003069"
     CDS             complement(4736..5278)
                     /locus_tag="KPG09_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBG0967657.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDNKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5426..5710)
                     /locus_tag="KPG09_003070"
     CDS             complement(5426..5710)
                     /locus_tag="KPG09_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0967658.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5773..6537)
                     /locus_tag="KPG09_003071"
     CDS             complement(5773..6537)
                     /locus_tag="KPG09_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG0967659.1"
                     /translation="MNNYLKFATYQLIGFTSSIPSLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(6819..7085)
                     /locus_tag="KPG09_003072"
     CDS             complement(6819..7085)
                     /locus_tag="KPG09_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG0967660.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTDIEKPFWGEQCPVKSCCED
                     KNLIHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(7128..7937)
                     /locus_tag="KPG09_003073"
     CDS             complement(7128..7937)
                     /locus_tag="KPG09_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0967661.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKEKL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEV
                     QNNKRPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
     gene            8250..8531
                     /locus_tag="KPG09_003074"
     CDS             8250..8531
                     /locus_tag="KPG09_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967662.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            8795..9049
                     /locus_tag="KPG09_003075"
     CDS             8795..9049
                     /locus_tag="KPG09_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967663.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            9094..9417
                     /locus_tag="KPG09_003076"
     CDS             9094..9417
                     /locus_tag="KPG09_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967664.1"
                     /translation="MATEIFTVKITPLEAQKMIKENIDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTEKTVIKATSTGSAIGVLKIDWGAGGNLLKRVKNIL
                     AEYII"
BASE COUNT         3591 a         1132 c         1566 g         3537 t
ORIGIN      
        1 aatatatatt atttatactg taaatttata gataataaag tataaaaaaa atatattttg
       61 atgaaattta aaaaaataca ttatataaat atagtaatag tatgaaaatt tacatataaa
      121 actaaaaaat gtaagtaatt gactttttaa gttgacaaaa tattgcatat atattatgat
      181 ataaatcata aattaactac aaatcaatat tatatattcg tagaagaaga aagtagcata
      241 aaggaatttt acagagagtt agatttggct gagaactaat attggaattt atgtgaaaag
      301 gactttggag aatactattg taattatgtt agaatagtgg tttaattcgc cttaagaaaa
      361 tgagtgagta aaattaattt ttactaataa gagtggtaac acggatttat attcgtctct
      421 tggatttttc caagaggcgt tttttattat aaacttattt cagaaaattt aaaactatat
      481 attaaatgaa aattataaaa atatgatagt taataaattt ataaattaga aaattgtaaa
      541 atatgctaag gtcagtagtt ttacgattta aatggaggga tgaaaatgga agaagaaaaa
      601 attaactatg ctctggaaca gattccagag aatcaaaaac gtggttgggt agctatgttt
      661 tcagttttag tagcaatagg tgtggattta tcatctgtta ttttaggcgc tgagttggca
      721 cagagtatgc caatgaaaca agcaatattg tcagtaattg ttggttcatt tttttctgca
      781 attttgtata ctacatgctc tttggttggt tcatctacaa gtttatctac atctatgata
      841 acaaagtatg tatttggaga agctggagca aaaatatttt ctctagttat aggggtttct
      901 ctattaggtt ggtttggagt tcaagtaggt ttctttgcac aaaatgcaca gataataata
      961 aaagatatat ttaatttaga tgtcagtatg cagattttaa gcctaatagg tggtctcttg
     1021 atgatgagta ctgcaattta tggatataag gccatggaga agttgagtgt gtattcagta
     1081 ccatttttat tagttcttat gatgctaact atattcttag cttttagagc aaatggaatt
     1141 tcagttgatg ataatatgaa atctactatg acatttgcag gtggagtttc tctgtcaatg
     1201 agtataataa tagttggtgc aattgtatct cctgatataa gtcgttgggc taaaagtaga
     1261 agagactgtg ctctttcaag ctttttggga atacaatttg gaaatgcttt tatgataata
     1321 gtttcgatag tattagtaaa atgtatggga acttcagata ttatgagaat ttttattaca
     1381 ttaggaattg ctataccagg tataatagta cttacattag cacaatggac tacaaataca
     1441 agtaatgtat attcagcatc attaagtata gctcttgtac ttaagaaggc tcctgagaaa
     1501 gtattaacta tagtccttgg tattatagca acattattgg ctgtatttgg aatatatgaa
     1561 ggttttatag gatttttaaa tttacttgga attgtaattg cacctgttgg tggtgtatat
     1621 acagcagaat attacatagt aaagcaagaa cttaaaggct ttgataaagg agtattatat
     1681 aaaccaatag ttaaacgttc aatagtgtct tggataattg gtatattaat aacttacctt
     1741 tcaacatatg gatttataac tcttactact atagcacctt tagatggttt tatagcaggg
     1801 tttgtagtac aatctattat tggaaaagtg ctttgttcaa caaaaaataa gcaagaaata
     1861 gacaaagcag tataaagaaa tttgtttata aagtataaaa taaattagaa atatgaggag
     1921 aaagtaacat gagattttta acagaagaaa gtgtagataa aatagcagtt ggtgcagcag
     1981 tgcttggaac tggaggtgga ggtgacccat atgtaggtaa attggttgca aaacaagcca
     2041 ttaaaaagta tggtcctgta aaagtaataa gtttagatga acttgatgat gatgctttgg
     2101 tagttccagt atctggtatg ggttctcctg taattacaat agaaaaatta ctttcagaag
     2161 tagagttaac taccccttta gaaataatgg agaaattatt aaatagaaaa gtggatgtta
     2221 taattcctat agaaatagga gggataaact cacttatgcc tatagcagtt gctgctaaaa
     2281 aaggacttcc tatcttagat gcagattcta tgggtagagc tttccctgaa gctcaaatgg
     2341 taacttttta tcttgaagga tatgaggcat ctcctgccgt aatgtctgat gaaaaaggta
     2401 attctgccat cctttatcct gtggatggaa tttggtcaga aagattagct agaactttaa
     2461 ctgtagaaat gggaggaagt tcttctatta gtgactataa tttaagtgga gctcaagtta
     2521 aaaaagctgc tataggtaat actcttacaa tagctgaaac tataggaggg tttttacttg
     2581 aaaataaatc tgattcaaaa ggtgctgttg agaatatact aaaagaattg tgtggataca
     2641 agttatttga aggaaaagta attgatatta agagagagtt gaaaggtgga tttactagag
     2701 gacatgcctt ttttgctggg attaatgagt atgatggaga gtattctata ttattccaaa
     2761 atgagaattt aattgccaaa aaaggtgata caccactttg tattacacca gatttaatag
     2821 cagtattaga tttagaaact ggatttccaa taactacaga gagaataaaa tatggctctc
     2881 gtgttatggt agtagctttt ccttgtaatg aaaagtggag aacagaaaaa ggaattgaga
     2941 cagttggacc tggatacttt ggatatgatg tggaatataa aacagtagaa gaattacaag
     3001 gaaaataaaa gtttaggagg aaattttgat gtatcgtgta ggaattgacg ttggaggaac
     3061 aaatacagat gcttgtatac tggatgggga attaaaagta attcattcag ttaaggtggc
     3121 aactacaaaa gatgttgaaa ctggtgttta caatgcctta aaaaaagtta tagatgaaag
     3181 taagattgac cattcattaa taaaatatgc tatgcttggt acaactcatt gtacaaatgc
     3241 tattgtagaa agaaaaagac ttaataatgt agctatgata agaataggaa agccggccac
     3301 tactgccatt cttccattta tagattggcc agaagactta agagaaaaaa tagaattaga
     3361 ttcaatattg gtatctggtg gatatgagtt tgatggtaga aagataaatg aactttgtaa
     3421 agatgaagtt gtagagtttt gcaatagaat aaaaggcaaa gtggaaagtg ttgcaataag
     3481 tggagtattt gctccagtaa ataaacaaca agaagaagaa gttgcaaaat gggttagaga
     3541 aattttaggg gatattccta tatctttatc aaatgaaata ggtaatatag gtatattaga
     3601 aagagaaaat ggagctattt taaattctgc attgtctcaa gtaggtaaag ctgtatcttt
     3661 aggatttgaa aaagcactta aagacttagg tatagatgct caaatatatt tcagtcaaaa
     3721 tgatggaaca ttaatgaatt tagagtatac aatgaaatac ccaattttta caattggatg
     3781 tggaataaca aattctataa gaggagcttc cttcttatca aaagtaaaaa atgcaatagt
     3841 attagatgtt ggtggaacta caagcgactt gggtgtacta tataatggat tccctagaga
     3901 atcatctatt ccagttacaa ttggaggagt acatacaaac tttagaatgc cagatgtttt
     3961 aactattggt cttgctggtg gtacatgtgt aaaaggcgaa aaagataata taaagatagg
     4021 tcctgaaagt gtgggatata gaataactga agatgcaata atatttggtg gagacactct
     4081 aactttatca gatgttgtta caaaattaga tatggcgttt gaagatagaa aaagcaatgt
     4141 tgaaaactta gataaagagt attgtaatga ggtatataag aaggttattt taaaattaga
     4201 agatgctgta gaccaaatga agactagtca agatgatgtt gaattggttt taactggtgg
     4261 aggaagtata attgttcctg aaaaactaaa aggcataagc aatgttataa gacctcctca
     4321 ttatactgct gcaaatgcta ttggtgcagc tttaggacaa ataagtggag atgtagaaaa
     4381 agtatacagt ttagataaaa tgtcgagaga agatgccata gaagatgcaa aaaaagaggc
     4441 tgtaaataaa gctctaaaag ctggtgctaa agaagatact atagaagtat taaatataga
     4501 agatgttcca cttgcatacc ttcctggtaa tgcacttctt ataaaagtaa aagttgttgg
     4561 tgatttaatt taaagaataa atataagtat ttccagaatt atataaatat ttctggactc
     4621 atttttaaac ttattttatc aataaaaaca gcataacttt tgactatatt cttataagtt
     4681 tccgaacagc cgatatatac ctattttttg aggtttatcg gctgtttgtt atcttttatc
     4741 gcattccaac tatttttctt accgtatttg ctgtacgaat tgtaatctta tcacttacat
     4801 ttgaactggc agtctttgac caccaatttg tctttttata atctttacga ctaaaagacc
     4861 aaaagatagc atttttataa tgatatactt tttcatactc ttctttagga tttccaacct
     4921 catcataaaa ctcgtcataa gataagtcag gaaacataaa aataagatta tcatatattt
     4981 ctttgttgtt atctccccac caattaggag cattatttag tatatctttt aactcctctt
     5041 gtgaaataat aaaaactggt atttccaatt caaactgttt ttttatcatt aatcttattg
     5101 tatttgagat aaccttgttg tcatctacat cacttaaaaa gacaacatta ccactattaa
     5161 ggtatgtaga aacttctgaa taaccaagtt ctacaaagct tgcttttaat tcagacataa
     5221 ttatcttgtt cttaccactt atattgatac ctcttaataa tgcgatataa cttttcatat
     5281 aataagttca cctctaaaca ttcacatttt tctatgtagt acaaactgga atttgcatct
     5341 tttataattc tttcaaaact tcttcatatg taattttttt aatataaaaa aatcggtgct
     5401 taatttgtac cgatttttat ttttcttagt aaagaactcg tttttttgct tctgctatag
     5461 ctctagcagc actttttgct tgcattcttt tttcttcttc ttttttaaat tcatttattg
     5521 tttcatttag attcttatca tgttcatttt tttctttatc ttcgttttct ctgtatacta
     5581 atatataatc tattattgat tcgtttatta tctgagatat acttttattt tcattaattc
     5641 ctattctctt aacttcctta agtagagtct catcaatatg aaccgttgtt tgctttgtat
     5701 tttttgccac cttaatcacc tcttaaaaaa aattctaaca ttaaaatttt ttttagtcaa
     5761 taaattttaa tattaaaatt ctttaaatgt tttatttgat tttctaaaat tttctattgt
     5821 atttatcatc ttatttttta tattttcacc tagtacagaa gactttagtc taggattttc
     5881 tatatggtca taaattggtt ctaaaaattc gacttcgaca ggaacagagt taattttacc
     5941 aataggttgg tatccttcat aagtatcttt tgaatttttt attacgagtg gaacaatagg
     6001 gcattttgct ttatatgcta tctttaaagc tccacttcta aactccgaaa ccaatgaatt
     6061 tggttcttta atccatgtta aatctccttc tggaaacact gccatacttt gacctgtaag
     6121 tatattttga gaggcttggg ctatactctt tataccctca cgattgtttt ttctgtttac
     6181 atatacacat cttagtaatt ttgcccattc tttaaatata ggtatatttc tccaaactgg
     6241 ttcatctgca attacacatc caattggtct ttctacactt gctgttagta tgaagctgtc
     6301 aagcatacta gaatgattta caacaaatag aagtggttct tttggcagtg tttccttacc
     6361 aattatatta agctctatgt tcactatatc taaagatttt ttagcttgtt tttgcataaa
     6421 ttcaaatttt tcctttaagc taaatttatc aggattcttt ttaattttaa taaggctagg
     6481 tatagaagat gtaaaaccta taagttgata tgtagcaaat tttaagtaat tattcataaa
     6541 attatctcct taaaataaaa ttatttatta ctgtaaagat tttaacataa aataaaattt
     6601 aagtctattt taattcaatt ttgtaacata tattttaaat agacagagat tgagatttta
     6661 tatttctaat tttagtaatc agattttatc aataaattac tattctatta atttaattaa
     6721 ataaccttta aatttaatta taaaaactgt tgcagaaatt tctacaacag tttaaaaaat
     6781 ttaccttaca aaaacttatt ttataacaag agagtatatt ataaggctag tacagcccat
     6841 tttttgcact gagaaattct tttgccatca tcaccttgtt ctttgtcata tgcaaattga
     6901 ttaagcattt cacatggaaa tgtatcacat agaccacaat gtataaggtt tttatcttca
     6961 caacaagatt ttacaggaca ttgttcaccc caaaatggtt tctcaatatc tgtacaacct
     7021 ttacaattga ctttttcctt gtatccacat tcataacata aaattccaca tcttgattct
     7081 atcattttaa gtcactcctt aaatataaat tttattatga ttgtatatca aaatttatac
     7141 ttttcattgt aaaaatcgga caaatttttt atataggttg ttggattaac accacaaata
     7201 gatttaaaat catttataaa atgagattgg tcgtaataac caatatcttg tgctaaagtt
     7261 gcaaatggtc ttttattgtt ttgtacttct tgtaatacaa ggtttatacg aagtaatcta
     7321 aggtatgatt taacactcat cccaagagaa ttattaaaaa ttctatttaa atgtcgttca
     7381 ctataacaac taatttgtga aatgttttta acagacataa tagagttctg ttttctagca
     7441 ttttcaagaa ttggtatagt catatctact atatttgatt tcaataagta tgataagaat
     7501 aacatatcta attgttctac taattccttt atagtacttg ttctttcaaa aatggaatta
     7561 acctctaagc taaataatgt gttaaaatct tcaagtgaaa tttttaaatc tgtagattct
     7621 ctttgagaaa gacctgtcaa ataatatact ccacctgggc gaaactcaac gaatactcta
     7681 aaaagtacat tttctatatc attttttaca atagtagttt ttgttgtggc tccccagaat
     7741 gcgctttcta tacctttttt gtcaaattta aatatcatac atccactagc atcaggtatt
     7801 aatgtaagct tttctttaat acttgtatct ggaatagata ttgtatagtg tgctatatac
     7861 tgtcttaaca atttatgtgg caaaatatat gtatactgtt tgcaggtatt taaaatgtta
     7921 ttgattattt tattcataag tatatcctcc tgatatataa aaaataagta tactaattta
     7981 ttttttatat aataaaccag ttttgtttta attgaaatat tctaataaga tattttgtta
     8041 aatttagata caataattgt aaagtaaaat ctcaaaatat acaattatta taaatcatag
     8101 atatttatta aataaatata ttaaagaaga aggaatataa aacaagatta gaaaataata
     8161 gtgttacttt cataataaat acatataaag atatttttaa gaataaaatt tattattaaa
     8221 taaaaaataa aggagagtga tttaatcgta tggcatcaaa acaaacacca agaaacaatt
     8281 tagaaaagtt tttaagaata agagtcttat cacatacagg cgataaaata catataaagt
     8341 tacctgtaaa ttttgcaaaa agaatgattg agaataatgc tttggattta tttaatggaa
     8401 aagatgatgt agtagatggt aaaaaggtta ctgaaatatt agtcaaagca tttgactatg
     8461 atttaactgg agaagtagtt cacttggaga gaaaaaatgg agatattatt aaagtttcaa
     8521 ttgtacaata gaattatttt atatgaattt tatttttgaa tagttataaa taattagaaa
     8581 tttgtaaaaa agtataatat attattagta atggtgcaga atacacattt ataatttata
     8641 gatgtgtatt ttttgtttaa aaaatttaaa tatataaaac tataaaggtt acaattttgg
     8701 aaatagatgt aaatatatgt tctattgtat aataagagta atataaaatc tagtaaaaat
     8761 aatcaaataa atttaactta tgagagggat aactatggaa aataaggatt ttaaattaac
     8821 aatatgtata tttgcttttg taattggagt agtagtatta ttctgctgta taggtttggg
     8881 tgaatttagt gcagagcata ttattcaaca aaatggtgga atcatggata ctgaccaata
     8941 taatatttat ctagaccaat ctatttctca atatagaaat tttggttcta tattagctct
     9001 gttaggagga atagggatat tgtttgataa atatatcaga aaatactaga gattatgttt
     9061 tgtctaaaat aaattcttat gaggagataa aaaatggcaa cagaaatatt tactgtaaaa
     9121 attacaccct tagaagcaca aaaaatgata aaagaaaata ttgatgcaga tttagttttt
     9181 agtgattctt ataatttagg agatgataaa tttatactaa ttacaacttt tgagaagtat
     9241 tacgcaagaa ataatagtga tgcaggatta atcgtagttt atgaaaatac cacagaaaaa
     9301 acagttatta aagctacttc aacaggcagt gcaattggag ttttaaaaat tgattggggt
     9361 gcaggtggaa atttacttaa aagagtaaaa aacatacttg ctgaatatat tatttaatta
     9421 ttttatctaa ctgataatat gtcaagaaaa tctcgtaaaa gaggttaaga atatttttat
     9481 aatatataga taaataagga tttgaaggta aagtttttaa tgtataaaga aaaataatat
     9541 aataaaacga attggcttta taattcttta taaggcaaag agtacatttc tttatataat
     9601 tgttggatat taaataatat gtaaatacac catttacata taaaagttta attaatatgt
     9661 taaaagaaaa tgaaatggaa attactaaaa aatactttta aattggataa tgattgatat
     9721 tacgtttata caaaaatcaa gtagaatttt atatatttta aatatgatag tttaaaatta
     9781 tgaaagaatg ataaattcca gtttgtaggg tagttttgat aaataa
//
LOCUS       DAELSY010000142         9798 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.142, whole genome shotgun sequence.
ACCESSION   DAELSY010000142 DAELSY010000000
VERSION     DAELSY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.142"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            204..938
                     /locus_tag="KPG09_003077"
     CDS             204..938
                     /locus_tag="KPG09_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967665.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSVVIEKSDHEKSAVSLENFR
                     RIDLGEYVGVEESRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            1149..2330
                     /locus_tag="KPG09_003078"
     CDS             1149..2330
                     /locus_tag="KPG09_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBG0967666.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGEVFNKVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMIILNVKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGILGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGIHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVEIVI
                     R"
     gene            2335..2886
                     /gene="mobB"
                     /locus_tag="KPG09_003079"
     CDS             2335..2886
                     /gene="mobB"
                     /locus_tag="KPG09_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBG0967667.1"
                     /translation="MKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGYRVGVIKH
                     TCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQLYEDMDLI
                     IIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLNDYKNISKY
                     IESCIKNEILSISNVEIKNILKE"
     gene            3005..3280
                     /locus_tag="KPG09_003080"
     CDS             3005..3280
                     /locus_tag="KPG09_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0967668.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIHAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            3536..5236
                     /locus_tag="KPG09_003081"
     CDS             3536..5236
                     /locus_tag="KPG09_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967669.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            5599..6786
                     /locus_tag="KPG09_003082"
     CDS             5599..6786
                     /locus_tag="KPG09_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967670.1"
                     /translation="MEAITAFCLVLISLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTIVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            6846..8009
                     /locus_tag="KPG09_003083"
     CDS             6846..8009
                     /locus_tag="KPG09_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0967671.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IEIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            8160..8963
                     /gene="zupT"
                     /locus_tag="KPG09_003084"
     CDS             8160..8963
                     /gene="zupT"
                     /locus_tag="KPG09_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBG0967672.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            9059..9619
                     /locus_tag="KPG09_003085"
     CDS             9059..9619
                     /locus_tag="KPG09_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HBG0967673.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
BASE COUNT         3821 a          967 c         1778 g         3232 t
ORIGIN      
        1 tatcgtaaat tgaattttca aaattcaact ttaaagtgtt ttttattaca gaaaaattac
       61 aattagcttc ttaatcttat taaattcaaa taaatattat ataattattt tataatataa
      121 taaatataat tattgaaaaa agagataact gtaaattgag atatagataa atactataaa
      181 atttaagaaa ggaggattga tttatgaagt taaaaaggtc attagtttgt gtaactattt
      241 taggtattat acttgttgga tgtcataaag aaaatactaa agagaaaaat caagtagcaa
      301 gtaaagcaac acagcaaaaa actatgacaa aagttcaaaa tgatgttaat gaaattatga
      361 ataaagatta taagtacatt ataaagaata tgggtatacc ttataataca ttttattata
      421 taaaacctaa agttttaaaa gaatcgaata caatgcaaga tataaataca tcaagttata
      481 tgacattagt ttatccaaaa tatactggaa atgatgaact agatggaagt gctttatatg
      541 ttgatattaa cgaaaataaa gttgtcaatg ttgaaaccaa ttctttctca tcacaaggta
      601 tatcagtcat tgatgctgaa tctagtgttg tgattgaaaa aagtgaccat gaaaaatcgg
      661 ctgtatcact agaaaatttt agacgtattg acctgggaga gtatgttggt gttgaagagt
      721 ctagaataaa tgaaattgta ggagatgcga attatgattt aacagcatat aatcatgaag
      781 gtagtaaagt agttaagagt tatagattga aagaagataa caaaatatta aaaaaagagg
      841 tacttacaat aagtattgtg gataacaaaa taaaatctat aaaaactatt gaaagtgata
      901 aaattgtaaa aataataaaa ggaactttat tagaataaaa tattctaaag catatgatgc
      961 tgtttcaaga tagaaggaaa tttctagctg ggaacagttt ttttattaga tttataaaat
     1021 ttgaactgca cttatagata actattagtt ataattaaat ataagataaa agtatttcca
     1081 acttttataa tctataatat attacataga aatatcttaa ataaaaaata tattggggag
     1141 tatttaaaat gatagaatta gaagatgcca taaaaattat tgaaaaaaat gttttaccaa
     1201 taaacagggt caagatagta aatcttatag atgcagtaaa taaagtagtt gcagaagata
     1261 tatattcaac aattgatagt cctccatttg ataggtcacc ctatgatggt tatgcatata
     1321 atgcaaattc tgaaaataga aatttaaagg ttatagggac tttgtatgca ggagaagtat
     1381 ttaataaggt attaaataaa aatgaagcag tgaagataat gactggtgga aagataccac
     1441 agggtgcaaa ctgtgtaata aaaaaagaag atgtaacggt tataggtgat atgataattt
     1501 taaatgtaaa attaaaggaa tacgataatt acatatttaa gggtgaggat ataaaaaagg
     1561 ggcaattgat tattaataaa aatcagaata taggatatga tggtattgga gtattagcaa
     1621 gtcttggaat atccaaagta actgtttatg atgatttaaa agttggaatt ttgaatacag
     1681 gtacagaact acaagatata aatgagactt tagagaatgg taaaatatac gatagtaata
     1741 gatatacctt atattcaaga ttattaaaac ttggaataca ggcagataca atatgcaatt
     1801 gttctgatga tatattggag ttagaaaaaa aggtaaagaa tatgttacag agtgtagatt
     1861 ttttaattac aacaggtggt gtttctgttg gagataaaga tttaattcca gatgtattca
     1921 aaaaaatagg ggcaacagaa ttattttgga agataaacat taaacctggt ggagcttgtt
     1981 atgtaggtat attgggagaa aagctattat ttggtctttc tggtaatcca catgcagcaa
     2041 tagtggtatt tgataatgtg atagttcctg ttattgagta tttaacatat aagaagggaa
     2101 tacatcatat aaaagctata tttaaaggag aatttaataa actttcaaat aaagatagat
     2161 ttgtaagtgg aaaactatat acaagtgaag gtaaattgta tgtagagcta aatgataaga
     2221 aagatgcaaa agctagatta tctgcaactc taagaagtaa ttgtatgatt aagattaaaa
     2281 agaatgagac aataaaagaa aatcaattag tggagattgt tattagatag ttttatgaaa
     2341 ggacacaatt ttatgtataa tatattgtca gttgtctcat attcaggagt aggtaaaact
     2401 acgttgattg aaggcataat aaaagaatta aataaaaaag ggtatcgtgt tggagttatt
     2461 aaacatacct gccatgattt tgatatggat gaggaaggaa aagatactta taaacataga
     2521 aaatctgggg cgagaaaagt ctgtataata tcagataaac gagtagcata tattgaagaa
     2581 ctaaaagaga agaatccatt atataaaatg attcaattat atgaagatat ggacttgatt
     2641 atcatagaag gatataaaaa ttatagattt aaaagattgg aagtaactag aaaaggtaag
     2701 tatgaggata ttttatctaa taagtctgat ttgataggta ttgtcagtga tatagaatat
     2761 aaattgaata ttgaacagtt taatttaaat gattataaaa atatatcaaa gtacatagag
     2821 tcttgtataa aaaatgaaat tttaagtata agcaatgttg aaataaaaaa catattaaaa
     2881 gaataatttt aagatgttct tcttatataa aatgatataa ttatttatat aaataaagat
     2941 ataagaaatt tatcaatttt attttgagtt ataaaatatt aaagaaattg taggaggaca
     3001 atatatgaaa aaaatattag tagcatgtgg agcaggaatc gcaacatcaa caatagtttg
     3061 tgacagagta gaaagattgg ttaaagaaaa taatatacac gcagaagttg agcaatgtaa
     3121 aattgctgaa tgttcaacaa aacaagaagg tgctgattta atcgtatcta ctactatact
     3181 accaacaact tatgatatac ctacaataaa ggcaacagga tatataacag gtataaatac
     3241 aacagcatta gataaaaaga tattaaatgc attgaagtag ttttgtgttt ataaaaaaaa
     3301 tttagtatta aatatagagt tgtatcttta taaaatttta aatatagaga tatagctctt
     3361 tttatgctgt ttcaagtata caactatatg atataaatct tcaattttat gaaaaagatt
     3421 gttattactt tgtaagttta taaaagattt aaagttattg ttatgttata ctaatttatg
     3481 ggtatttttt agaagtacta tatagaagta ctaataaagg agggatttaa tacttatgat
     3541 aaaacaattt gtcaaatatt ataagccata taagaaaatt tttacactag accttatagc
     3601 agcttttcta ttttccttat gtgatttagt ttatcctatg ataactagaa atataatgga
     3661 tgacgttgtt ccaaataaaa acttaagaat gttagtagtt tttgcagtag cacttatact
     3721 tatatttata gcaaaagcag ggcttaacta ttttatgcag tattggggac atgttattgg
     3781 tgtagatatg caagctgata tgagaaatga ggtatttaca cacttacaaa gacttccaaa
     3841 tacgtatttt gataataaca aatctggagt aactatgtct agaatagtaa atgatttaat
     3901 ggatataaca gaacttgcac atcatggtcc tgaagattta ttcatttcta tagtaatgtt
     3961 ggttggttca ttctttatac ttatagatat aaatattcct cttacgctta taatatttgc
     4021 tatattaccg tttataattt ggtttgcaat agctaaaaaa gataagatga atattgcttt
     4081 tatgaagagt agagttacta taggtgatgt aaatgctact ttagaaaata gtatagcagg
     4141 tatgaaagta actaagtctt tttgtactga gaaagaagag ttaaataagt ttgtaagaag
     4201 caataaatta tttagaagag caagacaaga ttcttataag gtaatggcag aatattactc
     4261 tggtatgaat ttatacatgg atatcttaga gtgggtagtt gtaatagctg gtggttattt
     4321 tacttatata ggaaaaatta ctttgggtga ttttgctgct tatatactat atgtaaaaat
     4381 gtttatacaa cctatgaaaa aattaataaa ttttacagag caatatcaaa atggtatgac
     4441 aggttttaaa agattcatag agataatgga acaagaccat caaaaagaag ctaaaaatcc
     4501 tattgaactt gaaaatgtaa aaggtgacat tgagatagaa aatatatctt tcacatatga
     4561 agataagact caagttttgg ataatttaag tttatctata aaagctggta agactatagc
     4621 attagttgga ccttctggag gaggaaagac tacattatgt aatctattgc caagatttta
     4681 tgaatttgat aagggtgata taaaaattga tggaaagagt ataaaagatg ttagtttaaa
     4741 atcattgaga aaaaatatag gtatagttca acaagatgta tttttattta caggaactat
     4801 aagagataat attctttgtg ggaaccctaa tgctacagat gaagaaatga tagctgctgc
     4861 taaaaaagct agaatacatg actttgtaga gactttgcca gatggatatg atacttatat
     4921 aggagaaaga ggcgtaaaat tatcaggtgg gcaaaaacaa agaatttcaa tttcaagaat
     4981 ctttttaaag aatcctccaa taataatatt ggatgaggct acatctgctt tagacaatgt
     5041 aacagaaaga gaaattcaag agtctttaga ggaattaagt aaagatagaa caaatttagt
     5101 tgttgcacat agattgacta ctataaaaaa tgctgatgaa atcatagtat tgacggataa
     5161 aggaattgaa gaaagaggta ctcatgaaga gttagtaaac aaaaatggag tatatagtag
     5221 gttacacaat aactagtata caaattttac accatagcta acatacgcat taaacttaca
     5281 aacataggat atattaagat tcataaagac aattcatttt atatttatga gatgttttat
     5341 gaataaaatg tatcctatgt tttatttttt gcaaattaca tttaattctc tttgtaagaa
     5401 tagagttaaa tagccaatgg tcaggataac gtttttacaa aaagaataat ttagaaaact
     5461 aactttaatt tatggaattt atcgaatttt atgaaaaaaa tcgaattttg acttgaaatg
     5521 tttttcaaaa gtagtaaaat aatggataac tatacataca ttttataaaa atacatttat
     5581 tatgaggggg gattttgtat ggaggcaata acagcttttt gtctagtttt aatatcactg
     5641 acgataggag atatagtttc agcaaaaact aaagcttttg ttccgtcggt ttttgtatca
     5701 gcaattatat ttatagtagg attctggact tttttcccag aaaatattgt ggacttagcc
     5761 gctttaggaa caccattagc tcaattaggt atgcttttgt taataactca tatgggaact
     5821 atgatgagta ttaaggaatt agcaggtcaa tggaaaacaa tagtaatagc cttggcagga
     5881 atagtaggaa tatgtgtagg tgctttggca ttagggacaa tagtatttgg atgggataca
     5941 gcagtaatag ctacacctcc tcttactgga gggctagttg catctataat gatggcagat
     6001 gctgctacag caaaaggtct tgcaagttta tcagtacttg caattttgat gtatgttgca
     6061 caaggatttg cgggttatcc tattacagca ttaatgctta aaaaagaagg aaaaagactt
     6121 ttatcagact ttagaagtgg aaaagtaact gtaaatacag atgaagaaaa agttaaggat
     6181 ttacctgaac aaaaaagcag atttaagata attccagact taccagaaaa atatgatacg
     6241 acatatatga taatacttag attaggattt gtagcatggt tagcagtaca atttacaaat
     6301 cttacaaatg aagttgtatc aagatatgtt atatgtttaa tatttggtgt aatagcatca
     6361 gaacttggaa tagttgatag aaaaccactt aataaatctg gttcatttgg atggttaatg
     6421 acatcattga tggcttttat atttgcagga ttagcaaaag ctacaccagc aatgcttgtt
     6481 gaaatggcag ttcctcttgt aggaatcata atatttggtg taataggtat gggaatattc
     6541 tctattatag taggtaagct tttaggtgaa agtaaagaaa tggctttttc agtttcgctt
     6601 acatcacttt atggttttcc accaaactat atattaactg aagaggcagt aaaggctttg
     6661 gctgaaacac cagaagaaaa agaattttta atggataaga tgcttccaaa gatgcttgtt
     6721 ggaggattta ctacagttac aatagcttct gtagtaatag caggtatctt tgtaaatcta
     6781 atatagtatt taagtgataa aaagtatttt aaataagaag aatgtgtact acttggaggt
     6841 atggaatgga tattaaagaa ataacaaaga gttataaaga ctatgtaatt aagcttagaa
     6901 gggaatttca tgaaaatcct gagaaaagta tggaagaagt tagaacatca aagagagtta
     6961 aagaagaact tgataaaata gagataccat atgtatcagc tggaggtaca ggtgtaattg
     7021 caactataaa aggagctaat ccaggaaaga ctgtagcact taggggtgat atggatgctc
     7081 tacaagttgt tgaatgcact gatgtagaat acaaatctaa aaatgaagga cttatgcatg
     7141 cttgtggtca tgatggtcac acatcaatgc ttctaggggc agcaaaagtg ttaaatgata
     7201 ttaaggatag tatcaatgga actgtaaaat tattttttca gccaggagaa gaagttggta
     7261 aaggtgcaag agctatgatt caagatggtg ctatggaagg tgtagatagt gtgtttggaa
     7321 tacatctttg gactgatgta gaatctggga ctatatcggt agaagaagga ccaagaatgg
     7381 cttctgccga tttctttaaa attactgtaa aaggtagagg tggtcatggt tcacttcctc
     7441 atcaaggtgt tgatgcagtg cttgcaagtt cagcaatagt tatgaatcta caatctatgg
     7501 ttagtagaga agtaagtcct ctggaaccat tagttgtgag tgttggagtt ttaaattctg
     7561 gaactagatt taacgtaatt gcgagtgaag ctgtcttaga aggtacaata aggctattta
     7621 atccagagct tagaaagcaa attcctggaa ttttagaacg tatagcaaaa tctacagcag
     7681 aagcatatag agcagatgct gaattagagt atgggtactt aacaccagca gttataaatg
     7741 acaaagaatg ttcaaagata gctacagatg cagcaattaa gctatttgga gaagactgta
     7801 taacactatt tgaaaaagta acaggagcag aagatttagc tgagtttatg aatatagcac
     7861 ctggtgcact agcatttgta ggagctagaa atgagtcaaa aggtgcttgc tatcctcatc
     7921 atcatggatg ctttaatata gacgaagatg ctctagaaat tggaactgct ttatatgtgc
     7981 aatatgcagt ggatttctta aataaataat tttaatatac tataaagaaa aagcagagac
     8041 taagtttata tctctgcttt tttattgtgt atatttttta taaatattga ctatataaaa
     8101 tatatatgat agcataaaga aaatgataat caatatcaat taaaaagaag gtgatttatt
     8161 tgaataatgt aatatttgca tttttgataa cattattggc tggtttatct actggtatag
     8221 gaagttgtat agcatttttt gctaaaaaaa caaatactaa gtttctttct ataagcttag
     8281 gattttctgc tggtgttatg atttatgttt ctatgataga aatttttcct aaagcacaag
     8341 atgctttgac aaaatctatg ggagagaaat taggaagttg gtcaactgta atagcttttt
     8401 ttataggtat ggctatcatt gctgttattg ataagttaat tccacaagaa gaaaatccac
     8461 atgaaataaa gaaaatggag aatatagaag aaaagaatat caaaaaaaat aagtctcttt
     8521 tgagaacagg tatatttaca gcaatggcta tagcaattca caattttcct gaaggattag
     8581 ctacatttat atcagctctt gatgatgtta caatagctat cccgatagca attgcaattg
     8641 caatacacaa tataccagaa ggtatatctg tatctgtacc agtttattat gccacaggag
     8701 ataaaaagaa agctttttat tattcctttt tatctggtat gtctgagcct ttaggagcga
     8761 ttattggata tgtacttctt agaaattttt taaatgatat aactcttgga attgtatttg
     8821 caattgtagg aggaattatg gtattcatat cactagatga attattgcca tcagctagag
     8881 agtacgggga acatcattta tctatttatg gattgattgc aggaatgggt gttatggcta
     8941 ttagtttatt actatttaaa tagcttttta aatatatgat tgtaacctaa aattagtatc
     9001 aaaatgatat aattatttaa atagattttt aagctattta aaaatatcat tttggagaat
     9061 gagtgctatg gaaaagaaaa taaaattcaa atttgactgg tatagttcac ctgataagat
     9121 agagaaatgg ttagaagata ttgaagctaa aggatataat ttatataaga taaataaatt
     9181 tggaggaata ttttattttc ataagggaag tccaagaaaa ataaaataca ctgttgatta
     9241 ctactatgat gtagagaata attattttga tgaacataaa tcaaatggct ggaatcttgt
     9301 atttaggagt attggtagtt ttatgggtac atctgcatgg tatatatggt cgaaagaata
     9361 caaagacata cctcctaaat tatatagtga ttctgataaa aaaaacaaag taaagtcaat
     9421 tttatttatg aatatgttta tattctttat gttagtagta atatttatat atttattgaa
     9481 tattgaaata agaaatttat atagtggtta tggagcatct gtaactgcta ttgtactaca
     9541 gattatattg ttgattttat atgcagtaca attatatagg agattaatgt attatttaag
     9601 gcttaaaaag tttaaataaa taaaagaagg tgtcctaaaa ttattttttt aatattttaa
     9661 gacatcttct tttgttagta aagtactgtt tttaattata gtaatttctg tctttaaatt
     9721 gaaatatcaa ataatagttg tattggattg agtattgata aaaataattt aaaaatgtta
     9781 tcctaaagta tcagatat
//
LOCUS       DAELSY010000143         9774 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.143, whole genome shotgun sequence.
ACCESSION   DAELSY010000143 DAELSY010000000
VERSION     DAELSY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.143"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            291..902
                     /locus_tag="KPG09_003086"
     CDS             291..902
                     /locus_tag="KPG09_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967674.1"
                     /translation="MNIKRINLLLSTVMAAVIISLPLAVTANAMEKTESDNNVKIELS
                     DKEVEELHNYWPEDKIFSDEKIDEILIKTGQFTQKELDKINQEVDDSAKKVNQNSRVK
                     YINGYNKYRTFTKNGKNYLYIYVSGKTLKKIKAGTDLASTFGGFLPKIWVELDVVAIG
                     KIITINMDGINTNYGIVLSYIEDKWNFQGATYTYRYDHWFYQT"
     gene            930..1112
                     /locus_tag="KPG09_003087"
     CDS             930..1112
                     /locus_tag="KPG09_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967675.1"
                     /translation="MKKRLYKVFLKTIPNLIIVILSLALSFYTNTFDIDLKILLLIPL
                     AYVLLFIYYYIKTKKD"
     gene            1352..1894
                     /locus_tag="KPG09_003088"
     CDS             1352..1894
                     /locus_tag="KPG09_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0967676.1"
                     /translation="MLIYLAILELEEDKIKFEKVYEKYRQIMFYVANKILKDDHLSED
                     AVHNAFLRIIKNIDKIDEVDSPRTKAFIVIIVERIAIDFYRKRKREKVSDIEEEYKNR
                     EINFSIEDKVCESNLAIALAKLNESYFQVLSLKFQYGFSNKEIANALNLGEENVYKRI
                     QRARKKLKEILEEMEVGNIE"
     gene            1926..2588
                     /locus_tag="KPG09_003089"
     CDS             1926..2588
                     /locus_tag="KPG09_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HBG0967677.1"
                     /translation="MLRESIPIATKIIIDDLLSDDELNHVFSKGFERKMKKLIKQQKR
                     SSLTNKIIFYSKQTAIVFLIVLASLLTITMSSEALRSRFFRLVIRVYEDLTSFVFSTN
                     GEDNLSLKNKEPEYIPKGFKEVDRVDDFVITYRNDENIEIQYVTDKISSNSIILDTEN
                     AKVKDIFVNGHKAKYIVKGTFLQLFWNDNNFIYLLNVDYQNSGESEFYEDILIKIAKS
                     IK"
     gene            2778..3095
                     /locus_tag="KPG09_003090"
     CDS             2778..3095
                     /locus_tag="KPG09_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967678.1"
                     /translation="MKSDLYLCKSNKLYRDNEVLEKFMEDLSFLIDDYKFSLETISIL
                     CKINIEKLTNFYAGKGYILYEEMSLIESVLAPFLSAIDIAKHNYDVVIKSRESDKPLI
                     FEN"
     gene            3117..3560
                     /locus_tag="KPG09_003091"
     CDS             3117..3560
                     /locus_tag="KPG09_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967679.1"
                     /translation="MKRLFALLLTLSLVILPSTLVFANEKAENDSIYAEAPLVFQSNE
                     KAYTHGSGGTAYIGYMSSKHLNWRITSSKGIVILFSGTVEIRNSKNMLVASYPISGGG
                     SSTISGQIDVKYLKKGDYKAILKGTSTTGGGVLVVKSGLVQMFTI"
     gene            3778..4221
                     /locus_tag="KPG09_003092"
     CDS             3778..4221
                     /locus_tag="KPG09_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967680.1"
                     /translation="MNLRKTVISGMCALVCSSAVAIPAFADEYQDKNIEQYSAVNYID
                     DTMVKMSYIKSGTCNLSISGSTAKVTCFVRGASNVTSTSIIARLQKSNDNGKTWSTIQ
                     TWNASGNISCSLSKSKTIGKGYYRVHSTIKANSESKNIISSTKKY"
     gene            complement(4352..4772)
                     /locus_tag="KPG09_003093"
                     /pseudo
     CDS             complement(4352..4772)
                     /locus_tag="KPG09_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            complement(4750..6027)
                     /locus_tag="KPG09_003094"
                     /pseudo
     CDS             complement(4750..6027)
                     /locus_tag="KPG09_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(6050..6592)
                     /locus_tag="KPG09_003095"
     CDS             complement(6050..6592)
                     /locus_tag="KPG09_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967681.1"
                     /translation="MLLCDPASAGGSKHDYSAFLVGSESENGLLCGRLAELAKINART
                     DFDKYILHMIYLLKVYPDITHVYIEKNTFNGTAANQLELKIKNDDVLYYRDIEIINEH
                     QKKNKDDKISTLIPVLNKGQMIFAEEDKAFIQQILNFTGQKYSLHDDAPDISAEFINR
                     IFNIKVNESITLLDRRNLGL"
     gene            complement(6632..6850)
                     /locus_tag="KPG09_003096"
     CDS             complement(6632..6850)
                     /locus_tag="KPG09_003096"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967682.1"
                     /translation="MLFDFKNTNHLEDAKEFYWENEKEIKFPILWPSFWNCLDMALSY
                     YENPTSFKQEVQNDVNSIGEKWFKTIRT"
     gene            complement(7046..7789)
                     /locus_tag="KPG09_003097"
     CDS             complement(7046..7789)
                     /locus_tag="KPG09_003097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967683.1"
                     /translation="MDNLERNIQLIFSYMAKTFIRNGATIREAEKEANEIIKTNSSNL
                     WGVNGLAYQLGKINLEFFCMYFIQDTYLPKEDNVAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGKKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKKFSDDVKYAA"
     gene            complement(8089..8646)
                     /locus_tag="KPG09_003098"
     CDS             complement(8089..8646)
                     /locus_tag="KPG09_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG0967684.1"
                     /translation="MVVGYMRVSTAEQNEDRQLVTMKKHNVEKMYQEKISAKDTKRSK
                     LNAMLDFVREGDTIIVHDFSRLARSTKDLLEIVELLEKKHVKLISDKENLDTSTPTGK
                     LMLTMLGAIYEFERTNMLERQREGISIAKEQGKYKGRKEVKIDNFGSYYDRYKTRELN
                     KTQLAKELGVSRPTLDRLIKSHESN"
     gene            complement(8841..9029)
                     /locus_tag="KPG09_003099"
     CDS             complement(8841..9029)
                     /locus_tag="KPG09_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967685.1"
                     /translation="MNNKKKSFLISGTINVILLIIVFALFSYNIIENSTRNMIITVIL
                     IFIFEIIKIKILEKYYNL"
     gene            complement(9040..9615)
                     /locus_tag="KPG09_003100"
     CDS             complement(9040..9615)
                     /locus_tag="KPG09_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967686.1"
                     /translation="MLKKKIISFLMAGIFVLSSGYNLVYASTFEENEYKQELVNENEF
                     TIINSNENEMIVDKKGNNTYVSTKVTPGSWKNVKYLGQKSRKLSSPYTRERIIQTIQW
                     GLGYLSGGSSFLLGLAESIYREKVYDITKYGGVYTKTYYRRQITDIRNSHANVPVPYR
                     YEYVTYFYSSSNFKKVVYTERVGDGIKLANN"
BASE COUNT         3471 a         1314 c         1241 g         3748 t
ORIGIN      
        1 ttgtataagt tatattctat tttgtatgaa gtcaattaaa aactgaacta tattaaaatt
       61 aattttataa ataacaaata taaataatta caaaacctta actgcgttaa gtttgaaata
      121 taataatatt aaaaacactt ctctcaaatc aagtatcttt tgttatattt tcataagttt
      181 taaaaatata aattttttaa aacttgtgtc caaaaaacaa aattaaatat gttatatatg
      241 taaagggtta attttaaaac cttaaattta ttatagttag ggggatttaa atgaatatca
      301 aaagaattaa tctcctttta tcaacagtga tggcagctgt gataatatct ttaccacttg
      361 ctgttacagc aaatgcaatg gaaaaaacag aatcagataa taatgtgaaa atagagttgt
      421 ctgacaaaga agtagaggaa ttacataatt actggcctga ggataaaatt ttttcagatg
      481 aaaagattga tgagatctta attaaaacag gtcaatttac tcaaaaggaa ttggataaaa
      541 tcaatcaaga agtggacgat tctgctaaga aagtaaatca aaattctcgt gtgaaatata
      601 taaatgggta taacaaatac agaactttta ctaaaaatgg taaaaactat ttatatattt
      661 atgtaagtgg aaagacatta aagaaaataa aagctggaac agatcttgca tctacttttg
      721 gaggtttttt accaaaaatt tgggtagagc ttgatgtagt ggcaattgga aagataataa
      781 caattaacat ggatggtata aatactaatt atgggattgt tttatcatat atagaggata
      841 agtggaattt tcagggtgca acatatactt atagatatga tcattggttc tatcaaactt
      901 aaaagacatg cttttttagg aggaataata tgaaaaaaag gctttataag gtttttttaa
      961 aaacaattcc aaacctgata attgtgatat tatcacttgc attatcattt tatactaata
     1021 catttgatat tgatttaaaa atattgcttt taataccttt agcatatgta cttttgttta
     1081 tatactatta catcaaaaca aaaaaagatt aatttttatg gaattagata tatacgaaaa
     1141 taattaattt aaaaatttaa ctttgagaat catttgtaga gttaaggccc ttataatccc
     1201 gaaaatggat ttgaagggcc tttttactag atatctttaa aaagcttgtt aagcatagtt
     1261 atattctatt taagtagagt taattatata tttatgttat aattgaactt taaaatatta
     1321 attcagaatt tttatattgg aggaattcta aatgttaatt tatctagcta tacttgaact
     1381 cgaagaagat aaaataaagt ttgaaaaggt atatgaaaag tatagacaaa taatgtttta
     1441 tgtagccaat aaaatattaa aggatgatca tttatcagaa gatgcagtac ataatgcttt
     1501 tttaagaata attaaaaata tagataaaat tgatgaggta gacagtccaa gaacgaaggc
     1561 ttttatcgtt ataatagtag aaaggattgc tatagacttt tacagaaaga gaaaaagaga
     1621 aaaggtatct gatattgaag aagagtataa aaacagggag ataaattttt ctatagaaga
     1681 taaagtatgt gaaagtaatt tagctatagc acttgcaaaa cttaatgaaa gttactttca
     1741 agtgctttcc ttaaagttcc agtatggttt ttcaaataaa gaaatagcaa atgcattaaa
     1801 tttaggggag gaaaatgtat ataagagaat tcagcgtgct agaaaaaaat taaaagaaat
     1861 attagaagaa atggaggttg gaaatattga gtaataaaaa agatgatttt gatattacgg
     1921 atgatatgct tagagaaagt atcccaatag cgacaaaaat tattattgat gaccttcttt
     1981 cagatgatga attaaatcat gttttttcaa aaggttttga gcgaaaaatg aaaaaactca
     2041 ttaagcaaca aaaaagaagt agtcttacaa ataaaatcat tttttattca aaacaaacag
     2101 ctattgtatt tttaatagtt ttagctagtt tacttactat tactatgagt agtgaggcat
     2161 tgcgttcacg tttctttaga ttagtaataa gagtatatga agacttaact tcatttgttt
     2221 tttcaactaa tggagaagat aatcttagtt taaaaaataa agaacctgag tatataccaa
     2281 aaggatttaa agaagttgat agagttgatg attttgttat tacatataga aatgatgaaa
     2341 atattgagat acaatatgta acagataaaa ttagttcaaa ttctattata ttagatacag
     2401 aaaatgcaaa agttaaagat atatttgtaa atgggcataa agcaaaatat atagtaaaag
     2461 gaactttctt acagttattt tggaatgaca acaactttat atatttatta aatgtagatt
     2521 atcaaaatag tggagaatct gaattttatg aagatatttt gataaaaata gcgaaaagta
     2581 taaaataatt tttttaagtg tccaagggta gcctcttcat tcgttataca agtatatatg
     2641 aaaatgtgag gtgattttta tggttttaaa atctacattt acctgagcgt taaaaatatt
     2701 gttataggaa aaattaatac attatagtta taacatgctt gcaataggaa taaaacttta
     2761 aaattgaggt gtaaaaaatg aaatcagatt tatatttatg taaaagtaat aaattgtata
     2821 gagacaacga agttctagaa aaatttatgg aagatttaag ttttcttata gacgattata
     2881 aattctcgtt agaaacaata tctattctat gcaaaataaa tattgagaaa ttaactaatt
     2941 tctatgcagg aaaaggatat atactgtatg aagaaatgtc tcttattgaa agtgtacttg
     3001 cacctttttt aagtgcaatt gatattgcta aacataatta tgatgttgtc attaaatcga
     3061 gggaaagcga taaacctttg atattcgaaa attaatttaa aactggggga atacacatga
     3121 aaagattatt tgcgttatta cttactttaa gtttagttat attaccatca actttagtat
     3181 ttgctaatga aaaagctgaa aatgattcaa tttatgctga ggcgccatta gtgtttcaaa
     3241 gtaatgaaaa agcatataca catggtagtg gaggaactgc atatataggt tatatgtcaa
     3301 gtaaacatct taattggagg ataacatctt caaaaggaat agttatacta ttttcaggaa
     3361 ctgttgagat tagaaattca aaaaatatgc ttgtagcatc atatccaata agtggtggag
     3421 gatcttcaac tatatctgga caaatagatg ttaaatatct taaaaaaggg gattataaag
     3481 ctatattgaa aggtacaagt acaacaggag gcggagtctt agtagtaaaa tctggtctag
     3541 ttcagatgtt tacaatttaa tattataagt aaaataaaaa tcatgaattt attgggggat
     3601 agtcacaata catattattt acagatattc ataactatta tagttaaaga cattctatta
     3661 taaagaaaat gtataattta aaattataat aaaaaaaatt atcattagtg tccaaaaatc
     3721 acttcttcat tcgttatata agttatataa atagaaaaca aatgaagagg tgattttatg
     3781 aatcttagaa aaacagttat aagtggtatg tgtgctttag tatgttcatc agcagttgca
     3841 atacctgcat ttgctgatga atatcaagat aagaatattg aacaatattc agcagtaaac
     3901 tatatagatg atactatggt gaaaatgtct tatattaagt ctggtacttg caatttaagt
     3961 atttcaggaa gtacagctaa agtaacatgc tttgttagag gagcatcaaa tgttacaagc
     4021 acatcaatta ttgctagatt acagaaaagc aatgataatg ggaaaacatg gtcaacaatc
     4081 caaacatgga atgcttcagg aaatatttca tgttctcttt ctaagtcaaa aactatagga
     4141 aaaggatatt acagagttca ttccacaata aaagctaact ctgaaagtaa aaatataata
     4201 agtagtacaa agaaatatta aaaatagata ttgtatttat gagttgtagt aaacaagaat
     4261 aatagttttt taaaagcaaa tataattaat tttaaataga gtgccaatat aaaataggtg
     4321 tataatattt atactttttt atattggcac tctattttat aaaataagtt attatagaaa
     4381 atgaaacata atttatatat aaattttctg tttgacctgg tatataatct tttatatcac
     4441 atatcagtat tttcccatca ttataacccc aaccaatcac agttgcaggt atcatatcag
     4501 cttctccatc ataaatgatt gtatgtttat acatgatttt tccctcgcta ttatctatag
     4561 ttttattcaa aataccttct gctattaatt tagcaactat gtctttaata gtctgtatct
     4621 gctttactgt ctacaaaaca tacttcaatt aatatcgctg gagcttttgt atgactaagc
     4681 caatacaatt cccttacgtc cgattttgca cctctatttt cagcagattt atatatatct
     4741 tcagtcaatt tagttataat cttttgattc atctaataaa atctctccct ctgaaactat
     4801 ttcctgttct tttttagcct tttccatttc tgcatgagga ttatcaataa aacttggttg
     4861 agataatccg gtttcagtag ataatttatc tccaagttga cttaatatat ttgctacaac
     4921 taaatcatta tgtggtatct taggggtaaa tttagcctta atatctttat aatcatagtc
     4981 aaaaccatat tttttattaa gccaaatgca caataatttg tacctattaa ataaagtatc
     5041 tgcaatacta tcattattct ggctacaaat ccattctgta ctgattaatc ttgctacaac
     5101 tgctaaactt gatgtattac tttgcatttt ttcattgtga tttatatgag atgtaatttt
     5161 atatatgttt tcttttattg aagatagtgt attttgaaca aatgtatcat tcatgtcttt
     5221 aataagccat tctatttttc catctccatt aacttgaagt attccaagtt ctttcatcct
     5281 tggcaaatca tcttctttaa tattacattc agagaaggtt aaataagcat ttctaaaatc
     5341 actaatttca ttacttatat cacttaaatt agtttcataa gcatcttgta atccttttat
     5401 atcattgaat attgtatttt ttcctagttc ttcacttctt ttacaaattg atattggaac
     5461 attaccatta aatatattat ttactgtagg tttttctatc tctttaaaat ttgaatcaaa
     5521 atgatatatg tattctttat catatacatc tatataaata tcatctttaa aatctttttt
     5581 aaattctcta aaaaaataga tggtctctcc atcttcattt tcataatggc agccatctgt
     5641 tggttttatt attcttactt tcatttcatt gttttttaca tagtaaagtt catagcaaaa
     5701 tccaaataat agtgtatatc tcaataaatc tgaatcgtgc tttttactcc aatggcaagt
     5761 atagtattct aaatcattta taatcttctc atcacctaat ctacttgtat aggtgatttt
     5821 atttgcaaga gaataagcga cctcttcatt aataaacttt tttaagaaat tagtgtttat
     5881 cttattatta gacctcttgg taaccatttt atagttacta atagcatctg tttcaccttt
     5941 ataatactta tacatagtgt tgtattcttg cttttttaaa ctctagtctt catatatttc
     6001 tcttaataaa cttaaatcta tttccattta atgcatcacc tccatacaat caaagaccta
     6061 aatttcttct atctaatagt gtaatacttt catttacttt aatattaaaa atcctattta
     6121 taaattctgc tgatatatct ggtgcatcat catgtagaga gtacttttgt cctgtaaaat
     6181 taaggatttg ctgtataaat gccttatcct cttctgcaaa aatcatttga cctttattta
     6241 aaacaggtat taaagtagag attttatcat ctttattttt cttttggtgc tcattaatga
     6301 tttctatatc tctgtagtaa agaacatcat cattttttat tttaagttct aattgattag
     6361 cagctgtacc attaaatgta tttttttcta tataaacatg tgttatatct ggatacactt
     6421 ttaataaata aatcatatgc aatatatatt tatcaaaatc agttctagca tttattttgg
     6481 ctagttctgc caatctgcca catagtaaac cattttctga ttcacttcca acaagaaaag
     6541 cactatagtc atgtttagaa ccacctgcag atgctgggtc acataataac attgtctttt
     6601 tgaatgtatg agtttctata cattctctag attaagttct gattgtttta aaccatttct
     6661 cacctattga atttacatca ttttgtacct cttgtttaaa agatgttggg ttttcgtaat
     6721 aacttaaagc catgtctaaa caattccaaa aacttggcca taatatagga aatttaattt
     6781 ctttttcatt ttcccagtaa aattctttag catcttcaag atgatttgtg tttttgaaat
     6841 caaataacaa ctctttgaaa ttaagccata aaccagaatt aaataattca tctacattat
     6901 caactaaaac tcctttttca catttaaatt tccaagttgg taaattcttt agcctactat
     6961 agaaacattc tttatgttgt aaggttccta atgctataaa agttgttcct ctttttataa
     7021 tcttaccatt tcttattact ggtttttaag cagcatattt tacatcatca gaaaattttt
     7081 taaatttttt ctctctagca tcttctgttc taacattttc ttctgactga taatcatcaa
     7141 gtatgataag atctggacga ttattattgt atttttttcc tctcattggt gatgaagatg
     7201 atattgcttc aataaaggtt ttattagtta attctaattg agtactattg caaatatatt
     7261 ttctatcatt atcatttaag agaactccaa atgctttttt aatatactca ttttcaagta
     7321 atgcattttt tatatctttt acgaattttt ctgctgtaga gcctatatca gagcaaatta
     7381 aagtatatgt cttatgttta taacaatgtg accatacagt tgttgctaaa tctccaaatg
     7441 cacttttacc tgttcctctt ggataaattc ttcctaattg ctgtgaacca tctccaatta
     7501 tagactcttg tatatcttcc cataactcat gatgaacttt agctatagga gcagcaacat
     7561 tatcttcttt tggtaaatat gtgtcttgta taaaatacat acagaaaaat tctaaattta
     7621 tttttccaag ctgataagct aggccattaa ctccccaaag attgcttgaa ttagttttta
     7681 taatctcatt tgcttctttt tcagcttctc ttatagttgc tccatttctt ataaaggtct
     7741 ttgccatata actaaatatt aattgtatat ttctttctaa attatccact cgtatttact
     7801 tcctcttaat tcttctattt ttttaggttc agtgagtaat acaattaata aaataaacaa
     7861 tgcaaatttt tttctaataa aatcaccctc tttgaaatat gtctctggtt tttaattttt
     7921 gctagaaaat ttctagaact cattttctat aaataatact tttttgaaat agaaggatac
     7981 cccctatact gaaaaacagt tatttataat tattttaaac ataattaaag ggcataattc
     8041 tatgaatttg ccccactaaa attaacttaa gcatatatat tgctcttctt agttactctc
     8101 atggctcttt attaatctat ctaatgttgg tctacttact cccaattctt tagccaattg
     8161 agtcttatta agttctctag ttttatatct atcataataa ctaccaaagt tgtctatctt
     8221 aacttcttta cgtcctttat atttaccctg ttctttagct atagagatac cttctcgttg
     8281 tctttctaac atgtttgttc tctcaaattc atatattgcg ccaagcattg taagcataag
     8341 ttttcctgta ggtgtacttg tatctaaatt ttctttatca ctaattaact ttacatgttt
     8401 cttttctaag agctcaacta tttctaataa gtctttagta cttctagcaa gtctactaaa
     8461 gtcatgtaca attattgtat ctccttctct tacaaaatct aacattgcgt ttagttttga
     8521 tcttttagta tctttggcac ttattttctc ctgatacatt ttttctacat tatgcttttt
     8581 cattgtaact aattgtctat cttcattttg ttctgctgtt gaaactctca tataacctac
     8641 taccatcttt gatacctcca cctatgttta ctctataaat aatattatag cataaatgta
     8701 aaactagagt taaagatttg ccatgctttt tgtaaattaa ttggagtttt gattttattt
     8761 ttacatcaga atagatagtt tgtaatgtaa atttgtacca gcatacttta caatatgctc
     8821 ttaggaaaga tatttcttgt ttatagatta taatatttct ctagtatctt tatttttata
     8881 atttcaaaaa taaaaattaa aattacagtt attatcatat ttcgtgtact attttctata
     8941 atattataac taaacaatgc aaaaacaatg attaataaaa ttacgtttat agtgccagaa
     9001 atcaagaatg attttttctt attattcata tatttcctcc tagttatttg ctagttttat
     9061 accatctcct actctttcag tatatacaac ttttttaaag ttagatgagc tataaaaata
     9121 tgtaacatat tcatatctgt atggaacagg aacattagca tgactgtttc taatatcagt
     9181 tatttgtctc ctatagtatg tttttgtata tacgcctcca tatttagtaa tgtcatatac
     9241 tttttctcta taaatactct cagctaaacc taataaaaaa gatgaccctc cagaaagata
     9301 acctaatccc cattgaatag tttgtataat tctttctctt gtataaggag aacttaattt
     9361 gcgtgatttt tgacctagat atttaacatt tttccaacta ccaggtgtaa cttttgttga
     9421 aacataagtg ttattgcctt ttttatccac aatcatttca ttttcattgc tattaataat
     9481 agtaaattcg ttttcattaa caagttcttg tttatattca ttttcttcaa aagtagatgc
     9541 ataaaccaaa ttatatccac tagataaaac aaatattcct gccattaaaa aacttattat
     9601 ttttttcttt aacataatat tttactacct ttacttaaat atttattcta taaacactgt
     9661 catataacct gttagttata ttaaaaataa tctttttata taacttcaca atcaaacctt
     9721 gtcttatttc ataatagttt tatttaatat catataattt agacctcctt ataa
//
LOCUS       DAELSY010000144         9758 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.144, whole genome shotgun sequence.
ACCESSION   DAELSY010000144 DAELSY010000000
VERSION     DAELSY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9758
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.144"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            280..501
                     /locus_tag="KPG09_003101"
     CDS             280..501
                     /locus_tag="KPG09_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBG0967687.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            1193..1423
                     /locus_tag="KPG09_003102"
     CDS             1193..1423
                     /locus_tag="KPG09_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBG0967688.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            1436..1585
                     /locus_tag="KPG09_003103"
     CDS             1436..1585
                     /locus_tag="KPG09_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBG0967689.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            1842..2279
                     /locus_tag="KPG09_003104"
     CDS             1842..2279
                     /locus_tag="KPG09_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBG0967690.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            2536..3429
                     /locus_tag="KPG09_003105"
     CDS             2536..3429
                     /locus_tag="KPG09_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0967691.1"
                     /translation="MQLYNTPFNTNKHLIYFFTSTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            3607..4431
                     /locus_tag="KPG09_003106"
     CDS             3607..4431
                     /locus_tag="KPG09_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0967692.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKHEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREVVPDYQSEGILWEKLSKFIKEEHIE
                     VSRQPNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            4530..5138
                     /locus_tag="KPG09_003107"
     CDS             4530..5138
                     /locus_tag="KPG09_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0967693.1"
                     /translation="MEKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            5242..5418
                     /locus_tag="KPG09_003108"
     CDS             5242..5418
                     /locus_tag="KPG09_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967694.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            6714..7466
                     /locus_tag="KPG09_003109"
     CDS             6714..7466
                     /locus_tag="KPG09_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967695.1"
                     /translation="MLKVNQLCKSYKTGNKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            7456..9567
                     /locus_tag="KPG09_003110"
     CDS             7456..9567
                     /locus_tag="KPG09_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0967696.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYI
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVAGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIERVYMKKSSYDMFFNSK
                     DFSKLIDKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         3763 a         1253 c         1248 g         3494 t
ORIGIN      
        1 gtacctaaca tttaactcta cacatatgaa aatccataca aaactatctc aactaagcat
       61 gaatatatac aaatactatt tcaaaacact aagaacatat cttcaaaaat aacttgtaaa
      121 ctttttcatc atcagaaaag taaaattttt cctataaaaa tttacacaaa aaatctttag
      181 aaattactgt ttaactccga tattatctta gatgatagta ttatagctaa caaagaaagt
      241 cgacaaatct ttgaaattaa atttttagga ggtaatttta tggaagttgt ttcaacaaaa
      301 aatttatcta gtataaaact aaaattggat gctggttttg atgataaggg taaggcagtt
      361 gttaaaagta aatcatttgc aaatgtaaaa gcagaggcat taaatgaaga tgtttatgct
      421 gttgcagaag caatagcaag tcttcaagag aaccctattg tagatatttt aaaattagat
      481 agtacatcat tatctaaata gtatctttta aacgtactct gctttacaaa ttaaaaaaat
      541 taactcttta tataaaagat taagttatat aacgcactta atacgctata gatttggcag
      601 attataactt caattttggt tataacttta atatattaat gctttgaaaa attaatatat
      661 taaacgtatt tcaacttaaa catgttagct attttaaatg ctaccctact ttaatctaat
      721 taattgaaat aaattaaata ttttctatat cagcaataac tagagtcaac aattaaaata
      781 ctaatattga caattgaaac actgatacta acaaacaaaa cactaatatt gacaattgaa
      841 acactgatac taacaaacaa aacactaata ttgacaattg aaatactaat actaacaagc
      901 aaaacactaa tattgacaat tgaaatacta atactaacaa gcaaaacact aatattgaca
      961 attgaaatat taatactaac aagcaaaaca ctaatattga caattgaaat attaatacta
     1021 acaagcaaaa cactaatatt gacaattgaa atactaatac taacaagtaa aacattaata
     1081 ttgacaattg aaatattaat actaacaagc aaaacactaa tattgacaat taaaatacca
     1141 atattaacaa gcaaacaatt aaaatataaa ttattatatc ggaggtaatt atatggaaat
     1201 taagaaaaaa ttagtcatga gctttaaaaa tgtaaaggat aaacaagttt ctttttctat
     1261 agaaaatcca aaggatgatt taactgaaga aaatataaaa tctgtcatgg atttgatagt
     1321 atctaaaaat attttttcgg ttggaggatt tgacctagct tctgttgttg aagccaaggt
     1381 tgtggaaaca aataccactc cttatgactt agttatagaa tagggggcta attttatgga
     1441 tgatagttta caactgttaa taaccaatgt tggttttcct atagctctat ccatgtactt
     1501 gttggttaga attgagggta aattaaatac tttatcaaac agtattgata cattatccaa
     1561 aaatattttg atatttaaga agtaatttga caatatatgt aagtcataaa ataggtctat
     1621 ttgtaaaata attagacata ttctatgcta aatactagga tatttggata atttatccaa
     1681 atatccatag tatttttttg aattttacca cctcaaattt gaatttcact attttatttt
     1741 tgaatttcac tatgcttaat ttgaaattta gaacgcatct agttggactc aataagatat
     1801 aatgctattc taatgacagt aataaaatga ggtgatgatt aatgaaaata catgtcgaac
     1861 aaggtattaa ttgtgatgat attgaaataa ccataaaatg ttctgctcta gataataaca
     1921 ttgaaaaaat aatttcatta ttgagtacac cattacttga tataaaaggt aaaaaatcag
     1981 gagagattta taaactcaat gtctgtgata ttttatactt tgaaacagtt ggaaataaaa
     2041 catttgctta ttatgaagat gatgtatttg aaattgattt aaaaatatat aaattatcaa
     2101 aattattagc taatacatcc ttctcacgaa tatctaaatc catgatttta aatatggact
     2161 acataaaaag tataaaagta atatcacata atcgcatgca agctacttta aaaaacaacg
     2221 aaaaagttat tataaatcgt cgttatataa aaaatattaa agaaaagcta aacatttaat
     2281 aaaaatctaa agtaatttat taatatcact aattgcaaat ttattgacct atttaataat
     2341 taataataat ataatatgtt caatattaaa tactaattaa ttatatttag tgtaaaaata
     2401 attaatcaaa atataaaaaa taggatatta ttataggcaa taactaacat caaacgataa
     2461 cttaatacat ataaaaataa ataaacaatt aataaaaatt aaaacttcat tataaaacgg
     2521 aaggatagtg aaaaaatgca actatataac acccccttta atacaaacaa acacttaatc
     2581 tatttcttta catctacgct agtattgaac tggatattca gtcttacacc tgcgttgctc
     2641 ggaattacaa atacaatact tggagaaatc attttttatc ttgcatatac atcaccagct
     2701 attgtaggtc tattttttat ttctacaatc tatccacaaa gtgcaaaaca agattttatt
     2761 tatagatacc taagctttaa agaaattaga ataaaatgga tactattaac aatattattt
     2821 tttgctttaa cttttattac ttcgttaata atcggaaatt actttaatgt aaaaacttct
     2881 gatatagatt ggatacacat agcagtgttt agaccttcta aaatagctta tatgctatta
     2941 gcaagtttta tattgagtat acttagtcaa gaagctggat ggagaggata cgccattgac
     3001 aaattattag ttagatttgg atttataggc tcttcaatta ttttagggct tgtttgtgga
     3061 atctggtatc tagggtcgta ttttacacct aatcaaatac catataattt ggcacaatac
     3121 tccctatttg atgctttttt atttatacca aacattgtac ttttaaactt tataattaat
     3181 tttgtatata tcaatacaag tagaagcatt ttatcagctg ggttagttca tatgatgtac
     3241 tattttttca acattcaatt acttctacac taccctatta aattatgtgt aatagctcaa
     3301 tatgtacaaa taatatttgg tttaatattt cttgtatata tgatttcttc taataaattt
     3361 aaacagaagg taaattctga aattgaacag ataaagtcgg atgattttga atttgagtta
     3421 gactggtaat tttaatatga attataaatt aagataatct atgatttgat acgattacta
     3481 cttaaatata atttactctc tataatttat tgcaacatct ggaaaataat tgtatttcca
     3541 caatcgcttg aagtatataa aaacaaaata tatactaatt ataagtgaaa aggaggtcat
     3601 attttcatgt ttaaaatagg agaggtttca aaactaacac aaatttctat tcgtatgttg
     3661 cgttattatg atgaacttgg tattttaaag cctgcagaaa tagacaaata tacaggtcat
     3721 agattatact cagttgaaca gatttcaata ttacaaagaa ttgtattatt gagagattca
     3781 aaatttagcg ttgcagaaat agcaaatatc gtacataatt ggaatgatga atttgtaatt
     3841 aaagaattga atagaaaaaa gaatgaaata caaaaggaaa taaagcacga acaacaaaga
     3901 ataaataaaa ttgataaatt tatagaagct ataaactgtg ataaagatga aattcattac
     3961 aatgttgtct ttaaaaagat tccaagttat aaaatcatat cacttagaga ggtagtccca
     4021 gactaccaat ctgaaggtat actctgggaa aagttatcta aatttattaa agaagaacat
     4081 atagaagtat cacgtcaacc caacaataat atagcttttt accatgatga agaagtcaaa
     4141 gataatggtg tagatattga agttggaatg gttgtaaaga agattggaaa gaacaaatct
     4201 ggatttatat atcgtgagac agaagaaatt gatatgatgg catgtactat ggtttatggt
     4261 ccatatgaaa atatagctgg tgcatatgaa tcgttctgtt attggttaga taaaaatagt
     4321 gattatcaaa tatcaggtat aaatagacaa atcggtcata agggtgagca taatgaaata
     4381 aatcctgaaa attatcttac agaaattcaa atccccctta taaaagtcta atcgattagt
     4441 acttaaatac ttaagtacta atcacccatt gacatcccaa aattttcatg atagtatcta
     4501 aacgtgtatt aaacatgttt aattttttta tggagaaact tttaagggga aaaattatga
     4561 aagcagattc aacaaaggaa caattaatta atgccacaat ccagttacta tcttcacata
     4621 gagatgtttc aagcattacg gctagagaaa ttgtagctaa agcaaatgtc aatttagcta
     4681 tgattaatta ttattttaaa tctaaagagg agttaattaa tatttctatt aaaaaatttt
     4741 taaagcgtca tatggatgaa tataaggcta cacatgatac taaaaattta gcacctaaac
     4801 agcaattaag ggaaatacta attgcatttt ttgattttat aggtgaacat agccaattta
     4861 taaagatttc tgtaccctat atattgatac aagaagaaat tgtttatcca tttgagatac
     4921 tacctttaat taaggctcac tatgaaaatc agaataacaa aaaaactgaa gctgaataca
     4981 gaactattgc gtatcaaata acttcattta ttcagttgct ttttcttcgt tctaatgctt
     5041 ttttcaagta tagtggtatt gatattctta atgttgaaca gagaaatggt ttaatagatt
     5101 ttcaattaga tttattattg gtagatatta caaaataata ctatattaat tttttatgta
     5161 cattttttta ttttatggta acgtttgaaa atattctcat tgactaaaaa tacttgcatt
     5221 aaataagggg gaggaatttt aatgtttttt acatttatag gagtaatatc agcaatttta
     5281 ttgttgtgta caattatatg tggtttatgg ataaaactta ataatcgtaa taatgactct
     5341 aattctcact ttactttttc aatactttca attgcttttt cactgttaat tgtggtggcc
     5401 tttttagtaa gaaattaatg tctttgttat gctggggttt attattatag atttttatta
     5461 ttgcatgatt tttactatat tatagtattt ttattatatt acagggtctt atttattata
     5521 gtgttttgtt attacagcat tttattatat tacagagtcc tatttattat aggattttta
     5581 ttatattgta aaatcttatt tattatagga ttttgttata ttacagactt ttattatatt
     5641 acaatctctt atttattgca gtgttttgtt attacagtat ttctattata ttgcaaactc
     5701 ttatttatta tggtgttttg ttattgcaac atttctatta tattataaag tcttatttat
     5761 tacagttctt tgttatacta cagactttta ttattacagc atttctatta caatctctta
     5821 tttattacag ttctttgcta tactacagac ctttattatt gcaacatttc tattacattg
     5881 caaagtctta tttattacag ttctttatta tactacagac ttttattatt acaacatttc
     5941 tattatatta caatctctta tttattacag ttctttatta tactacagac ttttattatt
     6001 acagcatttc tattacattg caaagtctta tttattacag ttctttatta tactacaaac
     6061 ctttattatt acaacatttt attattttaa cgtctttact ataatatagt aatattttaa
     6121 tatctcaaaa tcatatcaat cacttttcag catagtctaa aatacttcca ctttctaaaa
     6181 ttatctatca tacctatact aataatactt tttaaccttt ttattataca tttcaattgt
     6241 ttttaacgac ctatttattt ccaaaactcc aatatatttt ctcaattaca ttgtgttaat
     6301 aacccctaaa tcctttcaaa ttatttacaa tacattccat taaataatct tactgctcta
     6361 taattctttt tttcttttta accctactat acattattta catcattttt ttcaatacaa
     6421 cattcgttat tctataacct ttttactttt actatatacc ttttttctaa ctacaccttc
     6481 aatgttccac atcatattac tttagcacaa caattaatac ctaaaaataa ccctctaaat
     6541 cctccaataa tataatcaat ttaactataa taataacctc tcacactatc caaacaatat
     6601 attttcaaac attacaaaaa aacatccaac tattacaata ttgctcatat ccctcctccc
     6661 ctttcaaatg ctatcattga cttacaagat aaatgaaagt gaggatgata tatatgctta
     6721 aagtaaacca attatgtaaa tcatataaaa caggcaataa aacatatcct gtattaaaaa
     6781 atatttcttt tgaaatcaac aaaggagagt ttgtcgcaat catgggttca tcaggaagtg
     6841 gaaaaactac tcttttaaat tgtatttcat gttttatccc aattgataat ggggaaataa
     6901 ttcttggaaa taataatata agaaatttaa aagaaaagga actttcagaa gttagaaacc
     6961 aacaactagg atttgtattc caagatttca tgctatttga tggtcttagt gtctttgaaa
     7021 atatatgtgt tccacaaatt atcaaaggta gtcaagtctt acaaatggaa aaccgtgcaa
     7081 ttaatctatg cgagctattt ggtattgaac atatcagaaa aaaataccct gccgaaatat
     7141 ctggtggaga aaaacagcgt acagcagtag caagagcact catgaacaac ccagatttaa
     7201 tattagcaga tgaaccaact ggtaaccttg atagtaagtc ttgtcgtaca gtcattgaat
     7261 catttttaaa agcaaagcaa gaattagatg ctactatact catggttaca cacgatagct
     7321 ttgcagcaag cttctgcgac cgagttattg tgcttaaaga tggaataatt tataaagaag
     7381 ttgtaaaatc aaattcaaga cataaattcc tagatgaatt actagaagtt ttaaaagaaa
     7441 tgggaggcgc tcaagatgac aattaataaa ttatacagca aacttcgagc ctataattgg
     7501 aagaactata caactttatt cttttgtata atactttctg tggtattagt aacttcatat
     7561 gctctaattt attttagccc aacaatacaa aacatacttc caactggtgg agatagcaga
     7621 aaacaggcta caatgatatt ctcagttgct atattaggat gcgctgtctt tactacatac
     7681 gcttcttccc tctttttaaa atttaaaagc aaggaaattg gtattttcat ggctctagga
     7741 acagataaga agaaattaaa aaaacttcta ttttcagaga tattgttaat ttcaatagta
     7801 tgctgttttt taggtcttat attaagtatt cctgtatctt ttggtatatg gaaaatattt
     7861 caattagtga ttattgatac cagagaaatg acatatcatt ttggaactca aggattcata
     7921 tatggattta tatttagtgt atttgtgaca ttatgcatat ttatactttc tgtaaaattt
     7981 attaatcgta caaatataat tgatatatta aatgaccaga gaatatctga accaatacag
     8041 gatgtaaagg actggtatgg tatattaggt ataattttta ttatactagg tatttttcta
     8101 ggatatatcg taccttcact aactataaac aaattaaatt atagactacc atctgtatgg
     8161 agctttactt atctactctc tcttattgga atatatatga ttatgacata catagttgta
     8221 catgctaaaa gaggaaaaca tccagaaaaa tactataaaa acatcatatc aactagcatg
     8281 atgagattta tgggaagaca aacagtaaaa aacatgtgtg ttatttcatt tttgattgca
     8341 ggagcattat ttgctgtatt ctatgttcct aatatggttg caggtatcta tgatactctt
     8401 aaaaataacc ctatagatta tacttttacc tacaatcaaa gagataaaca ggtatcaaaa
     8461 aaagatattg aacaattagc agataaacac aatataaaaa ttcaaaacta tatggaaatt
     8521 ccttctatag aattaattgt tgatggtgta gaatcaatat atcataaaga tggaactatc
     8581 gaaagggtat atatgaaaaa aagctcctat gatatgttct ttaattctaa agatttttcc
     8641 aagctaattg acaaaccaat aactataaaa aatggtgaat atcttgctgt tgtaaataaa
     8701 ggaaatgaaa atcaagatta taaatatacc aaatttatat caagcccagt cacagaaaaa
     8761 gaaatcccct ttagatatgc tggaaaaata atatttaatg gttctttttt tcaaaataga
     8821 agtatgaacg cctatgtcct taatgatgag gattataaat catttactaa agatttatcc
     8881 ataaaaaatt acaataattc tatactattt aatgtagata aagataccta tgcttttgcc
     8941 aatgacttaa agaaagaaat tataaaaaga acatctaagg aaatgggcat gttcagtggt
     9001 tatgatgaat acgtaaaaaa acgttatgaa tcaactggaa gaaaatattt tatggatgaa
     9061 gaatatccaa gtggtgaagg tcatttaaaa ctagacccaa acaacaatca gttgtatctt
     9121 aattggaaat actatcctaa ctttaaaatt ttacaaagcc aagatatgat aaaaaacatg
     9181 gcagtcttct taatgttatt catatatgtg gcaatcatat gttttacagc agtaggaatc
     9241 attgcatata caaggggtgt tactatagct ataaatcaca agcaggtctt tatggattta
     9301 aatcgattag gagcaaacaa tgattatata aagttctgta ttagaaatca attaaagaaa
     9361 gtattttcat accctcttat tgcaggaagt tttttaattt atctgtttaa ttttatgatt
     9421 atgttaaata atgatggtag aatcgtcaat agtgaggtca atgctctttt gataaactta
     9481 ggttttatag ggatttgtgg agtttatatg taccttatat atctattaac ttataagaaa
     9541 ttcaaaaaaa ttattggtat tttataataa ggaaggttgt tagattttta aagtaataaa
     9601 atcattactt tttataataa gaaaagcatt agcattttaa attaaaaaag gttattagat
     9661 ttttatgata tgaaaagtca ttgtattttt atagtaagat aattatttgc tttttataat
     9721 atgaaaatcc attatatttt tatagtaaga taattatt
//
LOCUS       DAELSY010000145         9754 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.145, whole genome shotgun sequence.
ACCESSION   DAELSY010000145 DAELSY010000000
VERSION     DAELSY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.145"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1040..2398
                     /locus_tag="KPG09_003111"
     CDS             1040..2398
                     /locus_tag="KPG09_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG0967697.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     ASGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2504..3298
                     /locus_tag="KPG09_003112"
     CDS             2504..3298
                     /locus_tag="KPG09_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG0967698.1"
                     /translation="MVTIKEYSIPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3291..5597
                     /locus_tag="KPG09_003113"
     CDS             3291..5597
                     /locus_tag="KPG09_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0967699.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVAESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRIIRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5736..7013
                     /locus_tag="KPG09_003114"
     CDS             5736..7013
                     /locus_tag="KPG09_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0967700.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIESLKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7196..7918
                     /gene="yqeC"
                     /locus_tag="KPG09_003115"
     CDS             7196..7918
                     /gene="yqeC"
                     /locus_tag="KPG09_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBG0967701.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIFYGSVKQKFCVRY"
     gene            8025..8594
                     /gene="mocA"
                     /locus_tag="KPG09_003116"
     CDS             8025..8594
                     /gene="mocA"
                     /locus_tag="KPG09_003116"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBG0967702.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            8591..9610
                     /locus_tag="KPG09_003117"
     CDS             8591..9610
                     /locus_tag="KPG09_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBG0967703.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
BASE COUNT         3857 a         1013 c         1820 g         3064 t
ORIGIN      
        1 ataaatagta atatgaataa aatttttact ataaaaataa tattactatt ttgcttatag
       61 atatatatat ctataaatag atagaaactt gtatatttta tgttgcagat acaaatatag
      121 taaaggagag tacattggta caactgagat acatagctta ttaagttttt ctgtcttatg
      181 agaattatat tataaaatat tcataaatat tttgagttta aattaaataa ttattttatg
      241 cttagattaa ttgatattta attaatttaa taacaaaaac agtgaaagag taaataatat
      301 tatttacagg gggtaaagca aagtatgtta aataaattgt acttttggcc ccgtacatag
      361 agaaatctat gtaggacaca actttaaaac cagtaaatcc taaagccttg caactacaat
      421 aaaagataga aagaaacttt tatgaatgtg gtgaaaacca gaaacaatag tgaggatggt
      481 atatggtgag agcctaagta cttaaaaatg gacatttggt cgccaagcct agaaatagga
      541 aggtcaaacg actatctcga gagagagtac actataagcg attgatagtg gaaacgggtt
      601 gctcttagca ggtaatgctg aagatgaaga aatagtctac gctcatatga aagtatgagg
      661 tcactagatt tgaagtgaca tctataaagt agcgtttata ggtaaatgtt taaaacttga
      721 aacatgtaaa tacttatgaa aacttataac ttataatgat taagattgaa agatttaaat
      781 tgaattaaat ttactataaa aaattaaaaa ttcatagcta taatactctt atatttgtaa
      841 agcatggtat ataatgtata caaaattaaa gaatacgtgt atttgttaat aattaaattt
      901 attgcatctg ttttttaaga ctaaaaactt aaatatatgt tggagtatag ttgatattta
      961 tgaacatcat taatttaata tattagattt ttaagtattt atataattca ataagtttta
     1021 tgtaacggga gtttttatta tgaaagcaaa aagtacatca aaatttgaat tagacggaat
     1081 accaccacta aaggaagcaa tacctttagg gttacaacac ttaatggcaa tggtaatagg
     1141 tagttgttta ccagcgatat tgattgcaaa tgcatctgga cttaatcata agatgtcaac
     1201 attattgatt caagccgcat tagtttttgc aggattatca actttattac aattatatcc
     1261 aatacctttg tttaaaggtt ttaaattagg aggaggaata ccagttataa tgggagctac
     1321 agctatgttt ataggagctg gggtatctgt tgcaagtaag tatggactac cagtattatt
     1381 tggctctcaa ataatagctg gtatagtaat aattataatt ggttttggtc ttaaaaaaat
     1441 acgatttata tttactccag ctgtatcagg aacaatagtt gcatgtatgg gtcttggact
     1501 ttttccaata gctataaaaa acttagctgg gggtatgggc aatgagactt ttgggaatcc
     1561 aattaatttt atagttggaa tatctgtatc tcttatgata ttaggattaa ataagtatgg
     1621 aaaaggatta tttaaagatg catcaatatt agttagcata atttttggat acattttatc
     1681 attaattttg ggtatagtaa atttttcttc aattcaggag tttactttgg ttgcgcttcc
     1741 aaaaccactt gcttttgggc tagatataag acttgaagtt gttgtaatgt tttcaataat
     1801 atatttagtt gagatagctg atataatggg tgcttgtaca ctttctgcgg taggagggct
     1861 taatagacaa gtaacagatg aagaactttc ttctgctgtt ttaggaaatg gagtactctc
     1921 tacaataggt gcattatttt ctgcaactcc aatggggatg tttggacaaa attctgcaat
     1981 cgtttccaat acaaaggttg taagtaaatt tgtactagct attggaggaa ttggattatt
     2041 tttggcagga atatcgcctt tattagctaa tttaataaga acaattcctc cctgtgtggt
     2101 tggaggagca acactcgtta tattttcaac tcttacaaca tctggtttaa gacttgttag
     2161 tatggatgga tttaatcaag aaaatagcat gatattaggt ctttctatgg cttctggtat
     2221 tggattcatg gtagctcctc aagttcttga aaaatttcca aaatttatag agacattact
     2281 tgcagattca agtgtagttt caggagcaat ggtagccata ataattcaag ctttatatat
     2341 ggtaaaattt cctggaaata aaagtgataa ggttgaggat aaaaataaaa gtttataatg
     2401 taaaggttta atatatgtga attaaatata ctacaattat aaatataaat aaaagattta
     2461 ttaaaaactt ttgtgtttta ataagttaat gatgggggat aagatggtta ctataaagga
     2521 atattcaatt ccacagagtt tagaagaagc atataaaatt ttaatttcta aaaaaaataa
     2581 tgttatttta ggtggatgtg gatttataaa attaagtaat aaaaatatag gaacagcgat
     2641 agatttaaaa gatataaatc taaattatat aaaagaggat gaaaaaaata ttttgatagg
     2701 tgcagatact tcacttagaa gtttagaaat tgataaaact ataaaaaatt actgtagtgg
     2761 aatactttca agcgctgttt ctaatatagt tggtgttcaa tttagacaag gggctagaat
     2821 tggtgcaagt gtattttcaa agtatggctt ttctgactta ataccagcgc ttttggttgt
     2881 tggagcgaag gtgagattat acaataaagg aatactagag ttaagtgagt ttttggatag
     2941 tgaattaagt agggatatat tggtcgaagt aatactacca aaaaaagatg cgatagctgt
     3001 ttttgactca ataagaaaat gcacaggaga tttttctgta ttaaattcag caatgctaaa
     3061 agaaaatgat acttacaaaa tagcaattgg ggcaagacct caaaaagcta gattagcact
     3121 tgaagcgagt aatatattaa ataaagaaaa agatatagat aaggcatcta ttgttgctag
     3181 taaggaactt acttatggaa gtaatatgag agctagtaaa gagtacagaa aggatatggc
     3241 aagtgcatta gttaaaagga tgtataatgc tattgaggag ggaatgtata atgattaata
     3301 aggggataag aaagatagat agtacagcaa ttgtcacagg aaaacctcta tatactgaag
     3361 acctcattat gcataaagat gttttgacat taaaactact tagaagtcca catgctcatg
     3421 ctaagataaa gaatatagat acatcaaaag ccgaaaaaat tccaggggtt gtgggtattt
     3481 atacgtataa agatgtacca caggtcagat attgtgtgcc aggtcaaaca gaaccagaag
     3541 catcacctta tgatagagtt ttacttgaag atacagttag atttattgga gatgaagtag
     3601 ctttagtggc tgctgaagat gagaaaacag cacttaaagc cttaaaaatg ataaaggtag
     3661 attatgaggt tttaaagcct gtgtttgatg caagagaagc agaaggaaat gaagttgtta
     3721 tacatccaga aaaagacata gtagagccat acccttttgg attggatgca aaaaataata
     3781 tagtatcaaa agaatttttt gaatttggag atgttgaagc taagcttaaa gaaagtgatt
     3841 ttgtggctga atcttcatac aaaacccaaa gtcaatcgca ttgcatgatg gaaactcata
     3901 ggtcatattc atataaagat ataaatgata gaattgttgt agtttcatca ttacaatcac
     3961 cacacaatgt aaagagaata attagaaaag tattgggtat accatcttca aaaataagag
     4021 taattaaacc tagaataggt ggtggatttg gaggaaagaa tatagcagca acagatatat
     4081 ttactgcatt tgttactatg aaaactggta gaccagcaaa atttgtattt acaagaagtg
     4141 aaacttttgt aggttctaat agtagacatg aaatgttttt tgatgttaaa gttggtgcag
     4201 ataaagaagg aaatttaaaa gcaatagagc taaagtcatt aaacaataca ggtgcttatg
     4261 gtgggaatgg tgtctctgtt tcttccgaat ctggacacaa taccttacca atatataatg
     4321 atattgatgc tgttagattt aatgctagaa cagtatatac taataggtta ccagcaggag
     4381 cacttagagg gtatggtgct acacagggaa tatttgcaat agatagtgct attgatgaat
     4441 tggcacataa gattggaatg gacccacttg aatttagaat taaaaatata atagataggt
     4501 ataccaatgg aaaagcagct gaagacgaaa tattaagttg tgagctgaga caatgtatag
     4561 aaaagggcaa gaaattaatc aattgggatg ataaatatcc atgtaaagat ataggaaaca
     4621 acaaagtaag agcagtaggt atggcagtag caaatcatgg ttctggtata ccgcgtgtag
     4681 atatggcaac agtcactata agaatggatg aagatggcac atataaacta ttatctggtc
     4741 ttgctgactt aggtcaaggg gctgatacaa ttcaagctca aattgctgcg gaagctttaa
     4801 atactactgt agatagaatt tctatttata caggtgattc agatttatgt ccctatgata
     4861 caggagcatt tgcttcttgt acaacaacgg ttacaggaaa tgcaacaata aagacagcaa
     4921 ataaattaaa agatatatta ctttcagttg cagagtcaaa gctaggattt aaaaaagaag
     4981 atttagagtt gggaaaagac agggtttttg ttaaaaacga taatagcaag tttgttctat
     5041 tagaggaatt gggtagagaa tctgttggag gaataggatt tgctagtgat gatgtaccag
     5101 tagttttgga agcaacagaa tcttttggca tgttgaaaaa aataaaacca tttatagcag
     5161 gatttgcaga gatagaatta gataaaaata caggcgaata taaggtcata aattttgcat
     5221 gtgtacctga ttgtggaaca gttataaatc caactttagc tagaatacaa gtagaaggtg
     5281 gtgtgatgat gggtataggg tttgcaatgt atgaagaccc tatatttgat aaagatggga
     5341 gattattaac agatagtttc ctacagtata aagttcctac aaaaaaagaa gttggaaata
     5401 tattggtaga ttttgctgag aatttcgacc caggaggtcc ttatggtgct aaatcattag
     5461 gagaaatagt tatacataca ccagcgccag ctatagcaaa tgcaatattt aatgctacag
     5521 gagttcgttt aagagagctt cctatgactt ttgaaaaagt ttatatgggt atgaaaaata
     5581 ataataaaca attataataa ataataaggt gtataaaagc aagtaaattt aaattatttt
     5641 gtaaaataaa gtaattaaat ggacattata tatggtgttt tgtaaaaata ccgtatataa
     5701 tgtctagtag agtatattct aaaggaggtg agttcatgtc aaacagaata aaaaatatac
     5761 actatggaac tcagagacaa caattaaaaa acttaactaa aaaagaatat caagctctac
     5821 gagagatgtg cttcttagct aagaacttat ataatgttgg tacatataat gtaagacagc
     5881 attatttcaa tcaaggagaa catcttaact atgaagctaa ttatcatgta tgcaaagaaa
     5941 atgagaacta taagcttctc aatagtaata tagcgcaaca aatattaaaa gaagtagaca
     6001 gttcgtttag gtcatttttt gggttaatta aattagccaa agaagggaaa tatgacttta
     6061 gagggataaa atcacctaaa tatttagata aagaaggatt ttttagcata ataataggtc
     6121 aaataagaat caaagaagat gggattttaa atgtacctat gtctccagca tttaaaaaac
     6181 tatatgggaa agtaagtata aaagttccac aaaatatact agagaaaaaa attaaagaaa
     6241 taagaataat acctaaacat aaagctaggt tctttgaaat tcagtattgt tatgaaatac
     6301 cgaaatcaga ggaagtttca aatcaaaaaa atgcactagc tatagacttg ggtttagaaa
     6361 atttatgtac atgtgttacc aatttaggtg atagttttat catagatgga aaaaggctaa
     6421 aatctgtgaa tcagtgggca aacaaacaga atgataaaat agaatcctta aaattaaaaa
     6481 agaatataac tgcaataaca agaaaacaat ttaaagtttg gaataagaga aataatcaag
     6541 ttaaagacta tgtaaataaa acctgttttt atataattga ttattgtgta aaaaataata
     6601 ttggaaattt aattgtagga tataatgcta agttagaaaa aagtgataat atggagagaa
     6661 aaatatatag aaattttata agaatcccat ttggagagat aaaaggaaaa ttagaatatc
     6721 tatgtaaaat aaaaaatatc aattttatta ggcaagaaga aagctatact agtaagtctg
     6781 atttttttgc tgatgatttt ctacctgata tcaatgtgga caatgttaaa aaatatgact
     6841 ttaaagggga aagaataaca cgtggacagt acaaatccag tgttggaatc attataaatg
     6901 cagatgtaaa tgcgagtctt aatatactaa aaaaatcaca agtagttaat ttatctaatt
     6961 tacagaataa tcctcaaata ttaaagcaac cacagagagt aagaatatct taattatatt
     7021 taaaagataa actgatagcg taagcttaca gcttagcggc gaattggatc cgccttgttg
     7081 tttacaacag aatctattta ctttcgagtt tatggaattt caaaaatcac ttttactaaa
     7141 aaggtctaac tataataatt aagagattag aaaactggtt ataagaggtg aaagtgtgga
     7201 actatcaaat ataatagaaa agaatgaaat aataactgta gttggagcag gaggaaaaac
     7261 ttcgtttata aactattttg caaattttta tagagataaa ttgaaagttc ttcttacaac
     7321 aacaactaaa atatatgtac caaacgatta tgacaatata attattacaa tagatggaac
     7381 tgttattcca tctatttgtc atggaataac agtctgtggc agttatataa ataatgaaaa
     7441 taaattagta agtattgatt cctctatttt ggatgaaatt gtagaccaat ttgatttagt
     7501 tttaatagag ggagatggct caaagagaaa aaaacttaaa ggttggaatg ctaaagaacc
     7561 agtagtatat cataaaacta caaaaaccat agggatatta gatataacat catttggaat
     7621 gaatattaat gaagagaata tacatagagt agaaatattt aaaaaaatag caaatttaga
     7681 tacaagtagt attaatagta gttctacagt aagtattgaa aacttaaaaa atatagtatt
     7741 aaatccaaat ggattattta aaaattattc tggaaaaaga gtattattta ttaataaagt
     7801 tgaaaatgaa aaatataaaa atttagctat aaaattaata gaaaatatta aagaatatga
     7861 aagtgaaata gagatatttt atggaagcgt aaaacaaaaa ttttgtgtaa gatattgaag
     7921 tattaaaata gtttatatga tattatatat atagataata tcaatagaga tattataaaa
     7981 atcatagaac tatcaatata ttataaatac caaggatgat atatatgata aatgcagtga
     8041 taatggcatc aggctttgcc agaagaatgg gagcaaataa acttttatta aattataaag
     8101 gctgttctat aatagaacat gtatttagag aagttagcaa gattgatttt catcaagttg
     8161 tagtagtaag tcaatatgaa gaggttttgt tattgtgtaa taaatatggt tttgaatata
     8221 tagataataa aaatgcaaat ataggtcaaa gtgaatctat aaaattagga gttttaaata
     8281 gctcaaaatg tgatggttat atgttctttg taggagacca gccatttatt gataacttat
     8341 atatagataa aattataaat acttttaaat tagatagaga ttttatagta attccaagat
     8401 acaaagatat gtgtggaaat ccagtcatat tcccatatag taagaaagaa gaattgttgg
     8461 cacttaaaga agatgaaaaa ggtaaaacca ttttaaaaaa ttcatcaaaa ataaagtatg
     8521 tagaagttcc taaagaaatg ttatttgata tcgatacaaa aatggactat gagagaatcg
     8581 ggggtaaatt atgaagctaa taaaaactat agatgctgtt ggacaagttt tatgtcacga
     8641 cataactcaa ataatacctg gagaatttaa aggaagaaaa tttaaaaagg gtcacatagt
     8701 aaaagaagaa gatattccag tgttattatc tcttggtaaa gataatttgt atgtatggga
     8761 aaagtcagaa ggaatggttc atgaaaatga aggcgcattg tttttaaaag aactaactgc
     8821 tggagaaaat ttagatttta gtgaaataaa agaaggaaaa atagatttta tagcagcatg
     8881 tgatggtcta ttaaagatag atgttgatgc actttttgat ttgaattgtg ttggagaaat
     8941 aatgatggct actcttcata ataattttat agtaaataaa ggtttaaaag tagctggaac
     9001 aagagtgata cctttggtta tagatgagaa aaaattagaa gatgcaaaaa aagtagttgg
     9061 taatagaaaa atagtaaatg tagtaccttt taaacctaag aaagtaggaa ttgttactac
     9121 aggaaatgaa gtgttttata gcagaattgt tgataaattt ggaccagtaa tagaagaaaa
     9181 agtaaaaggc tttggttgtg aagttatagg acagactata tgcccagatg ataaagaaat
     9241 tataaagagt gcaataaaag aatttataag tcaaggagca gaacttatct gttgtactgg
     9301 cgggatgagt gtagacccag atgatgttac gccaacagct ataaaagaaa ctggagctga
     9361 tttagtgaca tatggttcac caatattacc aggagctatg tttttacttg cttattatgg
     9421 agaagttcca attatgggta taccaggatg cgcaatgtat cataaaacta cagtatttga
     9481 tatagtttta tctagggttt taattgatga aaagcttgat aagtatgata tagctagata
     9541 tggtcatggt ggcctttgta tgaactgtga tgtatgtact tatcctgcat gtaattttgc
     9601 taaaatataa agttattaaa aaagagtatt tacaaattaa gtactctttt taaatatgaa
     9661 acaaaacagc tagaatgatg ttttaagaaa agcatattta tttagaaatt ataataactt
     9721 attttcaatg tgtaaaagaa tgaataaagt tgca
//
LOCUS       DAELSY010000146         9751 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.146, whole genome shotgun sequence.
ACCESSION   DAELSY010000146 DAELSY010000000
VERSION     DAELSY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9751
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.146"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            498..1406
                     /gene="trxB"
                     /locus_tag="KPG09_003118"
     CDS             498..1406
                     /gene="trxB"
                     /locus_tag="KPG09_003118"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG0967704.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1496..3319)
                     /gene="typA"
                     /locus_tag="KPG09_003119"
     CDS             complement(1496..3319)
                     /gene="typA"
                     /locus_tag="KPG09_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBG0967705.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3531..3773
                     /locus_tag="KPG09_003120"
     CDS             3531..3773
                     /locus_tag="KPG09_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967706.1"
                     /translation="MLEVTKIIIKYFASESDTFIKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3921..4112
                     /locus_tag="KPG09_003121"
     CDS             3921..4112
                     /locus_tag="KPG09_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBG0967707.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            4403..6904
                     /locus_tag="KPG09_003122"
     CDS             4403..6904
                     /locus_tag="KPG09_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBG0967708.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNVLAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            7064..7732
                     /locus_tag="KPG09_003123"
     CDS             7064..7732
                     /locus_tag="KPG09_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0967709.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            7716..8993
                     /locus_tag="KPG09_003124"
     CDS             7716..8993
                     /locus_tag="KPG09_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBG0967710.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEKSELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            9394..9615
                     /locus_tag="KPG09_003125"
     CDS             9394..9615
                     /locus_tag="KPG09_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967711.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT         3879 a         1105 c         1649 g         3118 t
ORIGIN      
        1 tcatataaat ataagtaaat agtgctaata tttaaactat taatcataat tggttcaaca
       61 aaagtatata gttaataatg tttaatttga agaaattgtt agtgtgaaat tacatatata
      121 ttcaaattaa attcataaat atttcacata ataatagtat actgtaaaca aaaggaggta
      181 ttaaatatgg gaatagtact tataacagca gcattagttt tggttatgta tggactttca
      241 attctatcat atatatcaga aagcaatata ttagaagaca ctgtaaatga taaccaataa
      301 ggttttgaat tatataaata agtgagaact gtaaataaaa gttaattttt atttgcagtt
      361 tttttatata tatgtaaaat aaagttaatt aataaaaaat aatgttttgt ttgtagtata
      421 tatggataat aatagtaaag tataagaatg atgagaataa tatataagaa ataataatat
      481 aaagagaggt aaacattatg gtagatatca ttgtaattgg tgctggacca gcaggtttaa
      541 cttcagcaat ttatgctatg agagcagggt taagcgttac agtatttgaa aaaaatattt
      601 atggaggtca agttgcaagt acctctgagg tagaaaatta tccagcagtt caaaagattt
      661 ctggggtaga attttctaat aatatataca atcaagcagt tgctcaaggt gttgatattc
      721 agtttgatga ggttgaagag ataaatttag agggaaaagt aaaggttgta aaaacttctt
      781 caggtgaaca taaagcaaag gctgttattc ttgcaaatgg agttgaaaga cgtaaattag
      841 gttgtgcagg ggaacaagaa tttacaggta gaggagtatc ttactgtgca acatgtgatg
      901 gagccttttt taaagataaa gaagttgcta tagtaggtgg aggaaatact gcactagaag
      961 atgctttatt tttagctaat aattgtacaa aagtgtattt aattcacaga agagatagtt
     1021 ttagaggtga agaagtgttg gagaaatctg tgaaagcaag agagaatatt gagatacttt
     1081 acagtcatgg tgtagaaaaa atagaaggag aaaagactgt atcgaaaata gaagtaaaaa
     1141 acttaaagac tgaagaaaaa agaactatag atgttagtgg tatttttatt gctataggtc
     1201 ttaaacctaa taataaaatg tttgaaaatg ttcttgattt ggatgaaggt ggatatataa
     1261 tttcagatga gtcttgtaca acatcagtag aaggtgtata tgtagcagga gatagtagaa
     1321 ctaagttttt acgtcaaata ataacagcag catcagatgg agctattgca gccgttcaag
     1381 cagctaatta tataaatgtt gaataatata aatttcaaat aaaaatagcc atataaactg
     1441 aaataatttt tcagtactat atggctattt tgttaattag aggtaaatgt acgttttatt
     1501 taccttgtct agatttgtta tatcttaatc tatcatgttc atttaagaat ctttttctaa
     1561 gtcttataga atcaggtgta atttcaacta attcatcatc ttctataaac tctagtgctt
     1621 cttcaagagt aaatattcta ggaggagata attttatagc atcatctgaa ccagaagcac
     1681 gtacgttact cattttcttg tttttacaag ggttgactac catatcatct tttctggaat
     1741 tcataccaat tatcatacct tcgtaaactt ctacaccagg gtctacaaac attacagctc
     1801 tatcacttaa ggcattaaga gaatacccca tagtaacacc aggtccttgt gctattaaaa
     1861 caccatttcc tcttgaaggt atttctcctt taaattcttc aaatttttca aatgaacgaa
     1921 ctaaagtacc ttctccacga gtagcgttta taaattcact tcgatatcct aaaagaccac
     1981 gagtaggtgc taagaactct atttttacat agtctccttc tattgacata gactccatca
     2041 taccttttct catgtttaat tcatttataa tagtaccaga gtaaacttca ggacagttta
     2101 caacaactct ttctattggt tccattagtt ttccatcttc tttatgcatt aaaacttcag
     2161 gtttagaaac accaacttca taaccttcac gtctcatatt ttcaagtagt atagataagt
     2221 gaagctctcc acgtccagaa actttgtatc catcagtagt atcaagaggt tcaactttaa
     2281 gaccaacatt tacttcaagt tccttttcaa gtctatcttt caaatgtcta gttgtaacga
     2341 attttccact ttttccaacg aatggagagt cgtttactaa gaagttcata gaaagagtag
     2401 gctcttctat ttttatcatt tccataggta gtggactatc taaatcgcat atagtttcac
     2461 ctatagatat atcaggaata ccagctataa caactatttc tccactagta gcttcatcaa
     2521 cttcaacttg ttttaaacct tcatacacag aaagttttga tacttttcct ttagatacaa
     2581 cagaatcttc tctacatata gcaacttgtg tattgttttt aagagtacct ttgtaaattc
     2641 tacctatacc aagtctacct acataatcat catatgcaag agctgatatt tggaattgta
     2701 gaggttcatt atcgtaatta ggataagctt caacgtgatt tacaatagtt ttaaatagag
     2761 gagaaaggtc ttcactatca tcatccattt caagtttagc aataccttgt ttagcaatac
     2821 catatattat aggaaattcg cattgctcat ctgttgcatt taagtctaca aataaatcga
     2881 aaacttcatt aactacttct tccgctctct ggtctttttt atcaatcttg tttataaata
     2941 atattggttt taaaccgaac tctaaagatt tttgtaaaac gaatctagtt tgaggcattg
     3001 ggccttcact agcatcaact aatagaataa ctgtgtcaac agttttcata acacgttcaa
     3061 cttcagaaga aaagtcactg tgtcctggag tatcaactat gtttattttg taatcttcat
     3121 agtttattgc acagtttttt gaatatatag ttatgcctct ttctttttca aggtcgttac
     3181 tgtccataac gcagtcttta actacttcgt tctttctaaa aacaccgctt tgagaaagga
     3241 aagcatcaac caatgttgat tttccagcgt caacgtgggc tataactgcg atatttataa
     3301 ttttatgttt ttgtgacatt tttgttgctc ccttcgatta aaataactta ttaattatag
     3361 cacaatatat tattaggtac aaggaggaat atattaaatt gtattaatat agtttaaatt
     3421 ggataaaact gttagttttg catctgaaaa ttattctgtt taaaaaaatt ttttagacaa
     3481 ataataatct taatagataa aaagattaat aaaagagtaa aggatgatat atgttggaag
     3541 taaccaaaat aataattaaa tattttgcaa gtgaaagtga tacttttata aaagaagaag
     3601 tttgtaattt tgaagaactt cttaagtggt ctcataaatt tattgaaggt aaaaagatag
     3661 aagatgtttt gtatataaag tctaaagaaa actataaagt aaatgtgatt aagtctttag
     3721 aaattaagga attggaaata gtgagaaaac aacagatacc accgtacatg tagtattaat
     3781 aatatataaa attaatatgt aatactcaca ataggatata aatggtatat atggtaataa
     3841 tttatattaa aaaaactttt ataaagttta tatttgggta taaaagtatt aattagacat
     3901 taattaggag gaacttaaat atgataagag tatgtccaaa ctgttcaaat gtggatgtaa
     3961 ataagctaaa aactttggtt ggagaagaaa atgtaaagac tggttgtata ggtcagtgta
     4021 gagcatttaa aaaagaagct gttggaataa tagacaatga gcttgaaata aaggaaacag
     4081 aagcagagtt ttttgaagca tgtaaagaat aaattttaaa taaaatataa attataataa
     4141 atagagcatc taaattgtat aagcattaaa caatttagat gctctatttt tatatataca
     4201 tttaattatg agtatacttt tctattaaat acaaataaat ataactaata catttattta
     4261 ttaaaaaaat attgatttaa tatacctata gggggtataa taaaaatata gatttctaaa
     4321 taagtaattt atcaatatta catatcaaaa taaaacgtta caaattagct gaaaatatat
     4381 tgaaagaaag gaagatttta ttatgagttc aaataaagtg gtagaaagtt ataaaattac
     4441 aggtatgaca tgtgcagctt gtgcaaaggc agtagaaaga gtaactaaga agatggatgg
     4501 agtatatgac caaagtgtaa atatagctac tgaaaaactt aaaatagaat atgataattc
     4561 taaggttaat tttgatgata taaagcaggt tgtagaaaaa gcaggatatg gaattataaa
     4621 ggaagaaagt aataaaaaaa ttgatatgaa aattgatgga atgacatgtg cagcttgtgc
     4681 aaaagctgta gagagagtgg taaaaaaatt ggatggagta gaaagtatta gtgtgaatat
     4741 tgcaacagat aaagctaata tagactatga cccatcaaaa gtaaaattat ctcaaataaa
     4801 agcagctatt gaaaaagctg gatataaacc aatagaagag gtaagaaata aggttgatgt
     4861 ggatgaagat aaattgagaa aagaaagaga aatgaagtct ttatttgtta aatttatagt
     4921 tgcaatagta tttgcagtac ctctgtttta tatagcaatg ggacctatga taataaagcc
     4981 tatagggcca tggcctttgc cagaaattat aaatcctatg acaaatactt ttaattatgc
     5041 attaattcag ttaattttag taataccagt aatgatagct ggttataaat tttatataaa
     5101 tgggtttaaa tcactttttt ctttaagccc taatatggat tctttagttg caataggaac
     5161 attagccgca tttttatata gtctatatac gacactacaa atagccaatg gacaaattca
     5221 aggtatgcac catcatcagt tgtattatga aagtgcagga ataataatag cacttatctt
     5281 acttggtaaa tatttagaat caaaatcaaa aggaaaaaca tcagaagcaa ttaaaaagct
     5341 tatggggcta caacctaaaa ctgcaatagt gttagttgat ggtaaagaag ttgaaactcc
     5401 tatagaagaa gtagagatag gagatatact attagtaaaa ccaggaacta aaatacccgt
     5461 ggatggagtt gtaatagaag gatatacatc agttgatgaa tcaatgctta caggtgaaag
     5521 tataccagta gaaaaaaatg ttggaagtaa ggtgacagga gctagtatta ataaaaatgg
     5581 tgttattaag tttaaggctg aaaaaattgg tggagatact gctcttgccc aaataataaa
     5641 gttagttgaa gatgcacaag gtacaaaagc acctattgct aaacttgccg atactgtatc
     5701 tggatatttt gtaccaatag taattgcaat agctgtagtt gcatcattat tgtggttttt
     5761 aataggagga aaagatatag tatttgtact tactatattt atatcagtac ttgtaattgc
     5821 ttgtccttgt gcattaggat tagcaacacc aactgctata atggttggga ctggaaaggg
     5881 cgcagaaaat ggaatcttga taaaaggtgg agaggcacta gaatctgcac ataaagtaaa
     5941 tacagtgata tttgataaga caggtactat aacagaagga aagccaaaag taacagatat
     6001 agtattaaat aataatgtaa aagaagaata tcttatcaag attgcttcaa gtgcagaaaa
     6061 aggttcagag catcctcttg gagaagcaat agttaagtat ggagaagaga agaatataaa
     6121 atttgaaaaa gttgataatt ttaaagccat accaggtgct ggtatacaag ttactattaa
     6181 tgatgaaagt atattacttg gaaatagaaa gcttatgaat gacaataata taaagcttgg
     6241 ggatttagaa gaaaaatcaa atgtattggc aagccaaggt aaaacaccta tgtatatagc
     6301 tgtagatgga aatttatcag gtataatagc agttgcagat gtagtcaaag aaagtagtaa
     6361 aaaagcaata gaaatacttc atgatatggg aataaaagtt gcaatggtaa ctggagataa
     6421 tgcgaaaact gcaaatgcta tagcaaatca agtaggtata gatatggttt tggcagaggt
     6481 tttaccagaa gataagtcaa aagaagttga aaaactacaa aatcaaggaa agtttgttgc
     6541 aatggtaggg gatggaataa atgatgcccc agctcttgca aaagcagata tagggatagc
     6601 aataggaagt ggaacggatg tagccattga atctgcggat atagtactta tgaagagtga
     6661 tttaattgat gttccaacag caattaaact tagtcatgaa actataaaaa atataaaaca
     6721 aaacctattt tgggcatttg gatataatac gattggtata cctgtagcag caggaatatt
     6781 atatgtattt ggaggacctt tattaaaccc aatgattgca gcagcagcaa tgagcttaag
     6841 ttcagtttca gtcgtatcaa atgcacttag acttaaaaac ttcaaggcat ataagagaga
     6901 ttagattaat aaattaaata aaatgtatcc aaaagagcta tctttaaatt aaaaggatta
     6961 atttacaaat taagatagct cttttttcat gtaaaattca caacaataag ataaaatata
     7021 agtaatatac gaaattttaa ataaaataga aaggattgat agaatgaatt cgtcaatact
     7081 tgtaattgaa gatgattcaa atatacaaga gttaatatca gaatttttaa gtgcagaagg
     7141 ttatcaagtt gatacagcaa atgatgggtt ggaaggtata caaaagttta agcaaggaag
     7201 ttatgattta gtaatattag atataatgat gccaaactta gatggttatg gagtttgtaa
     7261 aatgataaga aagtcatcaa gcgttccaat tatattttta actgctttaa atgatgaagg
     7321 agaccaactt aaagggtttg acttagaatg tgatgattat ataacaaaac cattttcatt
     7381 taatcttctt ataaaaagag tagaagcaat tttaaggcga agtaacaaga ctataaatga
     7441 caaatttata gtttttgaaa aattaaaatt agatttaaac acatatattg cagagataga
     7501 tggagaacct atagaactta cactaaaaga gtttaatata ttaaaagctt tgatagaaaa
     7561 gtatccacaa gttataacta gggaaggtct tttggatagt atatggggtt atgactatta
     7621 tggagatact aggattgttg atgcacatat aaaaaatata agaaaaaaaa tatcgttacc
     7681 atatattaaa actgtaaaag gaataggata tacacttgag aaggatattt gataagtggg
     7741 aaaagttaag tataaagtat aagttatttt caataacgac atcattatta atagcgttag
     7801 ctttaataat atatctaatt ttatattttt tattgccatc atattatcat gagtataaaa
     7861 ttgagtctct tcaagaaagt ttaaaatctt tagtagatag ttcaattcat ttcgatacat
     7921 atactttaga agaaagactt tattatatgg ctaaagacca aaatttggct attttattaa
     7981 aagataacca agggaaaatt gtatatggta agaatgaagt agttatttta agatatagta
     8041 agtacatgat taatagtctt gaggatgagt atagaacttc tatacctata tatactaaag
     8101 atgcaaaaga tggtccatat actttagaac tcgttatgcc attacaacca attgacgaag
     8161 caaacgaagt tataagaaaa ctaatgccat acatcatatc aatagcaata ttgatagcga
     8221 ttattggagc atatatatat tcaattgtaa taacaaagcc tcttataaat ataatagaga
     8281 gtgagagaga acaggaatat agaaggaagg attttgttgc tacaatatca catgaactaa
     8341 aaactccaat aaccataata agtggtcaaa ttgaaggtat gatttatagt gtaggtaagt
     8401 ataaggatag ggatacttat ctgaaaaaat catatgaatg tacacaagaa ttaaaagatt
     8461 tagtaaatga aatgatagag gtttcaaaat cagaaatact tgaaaaagat ttgaaattgg
     8521 tatcaataaa tataagtgaa ttattaaata gacttgtaaa gagacaagta tttttaatag
     8581 aagaaaaaca tatgaaaact attttaaaaa tagaagaaaa tttggaaatt aaagcagacc
     8641 aagagagaat aactaaggca atcaataata taataaataa tgccataaaa tattctcctg
     8701 aaaaatcaga gcttataatt agactctatg ataaaaataa acgaataagt aaaaagactt
     8761 ctaatcaaag agtggtttta gaaattgaaa atactggtgt aactatcgag aaacgttatt
     8821 tagaagagat atttaatcca ttttatagaa tagaaaaatc ccgttcaaga aaaactggag
     8881 gaagtggact tggactttat atagtcagtc aaatttttaa aagtcatggg tttgattata
     8941 gcataaaaaa caaagaaaac tctgtagtat tcacagtaga atttaaaaat taattactta
     9001 ttataaagaa tatataactt tcaataatag ttcattttaa aatcacaaaa aattcataat
     9061 gaaactgtat tatataaata taaagaagct taaaaccata aaatacacca actataatga
     9121 tgagatttcc ttacatcctc catataagtc atcattatag taacccaaca attaagttac
     9181 tatgaattta aaaaataaca ccgagcatat aatctatgct tagtgttatt tttattttga
     9241 aataaaaaat atttaattta ttgaaatgta tagatttaga cagtttaaac tatatttaag
     9301 acttaaaaat tataattgat attacaaata aatatatgta ctttgggaaa aataaatata
     9361 ggaagtatta tatttacaga ggaggtagtt ttgatggata gattaaaaag agatatgaaa
     9421 aatatgaaag aagatttaaa agaaggtgtt aaaaagacag cttatgttat gaaaaataca
     9481 gctgaagatt tgaaagatga tgcacaaggt gctatggtaa acttagaaat gaaaaaagat
     9541 gagatgatgg aaagatatga acaaaagaaa ttttctaaag aattagagaa gcaaatgaag
     9601 aatgaaatga aataagcttt tatttatgaa taacaaatta ttgtattcat ataaaaagta
     9661 gccagtaata ttaaatagtt tatttaagga aatagacttt ctaaaaataa ctgtattaga
     9721 tttagaaagt ctatttcaaa ttagttttat a
//
LOCUS       DAELSY010000147         9627 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.147, whole genome shotgun sequence.
ACCESSION   DAELSY010000147 DAELSY010000000
VERSION     DAELSY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9627
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.147"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            213..1172
                     /locus_tag="KPG09_003126"
     CDS             213..1172
                     /locus_tag="KPG09_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG0967712.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDIKRFKAII
                     ATSLVLGVLGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            1287..2072
                     /locus_tag="KPG09_003127"
     CDS             1287..2072
                     /locus_tag="KPG09_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBG0967713.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDGLEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            2364..3683
                     /locus_tag="KPG09_003128"
     CDS             2364..3683
                     /locus_tag="KPG09_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0967714.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMSIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(3758..4186)
                     /locus_tag="KPG09_003129"
     CDS             complement(3758..4186)
                     /locus_tag="KPG09_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967715.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(4277..5653)
                     /gene="hydG"
                     /locus_tag="KPG09_003130"
     CDS             complement(4277..5653)
                     /gene="hydG"
                     /locus_tag="KPG09_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBG0967716.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(5654..6739)
                     /gene="hydE"
                     /locus_tag="KPG09_003131"
     CDS             complement(5654..6739)
                     /gene="hydE"
                     /locus_tag="KPG09_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBG0967717.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSPDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNKDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(6775..7983)
                     /gene="hydF"
                     /locus_tag="KPG09_003132"
     CDS             complement(6775..7983)
                     /gene="hydF"
                     /locus_tag="KPG09_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBG0967718.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVNKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(7998..8249)
                     /locus_tag="KPG09_003133"
     CDS             complement(7998..8249)
                     /locus_tag="KPG09_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBG0967719.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(8630..>9627)
                     /locus_tag="KPG09_003134"
     CDS             complement(8630..>9627)
                     /locus_tag="KPG09_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBG0967720.1"
                     /translation="LDIPSPAGYTENAVLEIKKDFENLGLDTILTKKGALIATLPGED
                     DSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGENCTVITRKGKKIRGS
                     VVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLGISVGDFVCLDHRFEA
                     TESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYISNYEEIGHGVSKCLPE
                     KTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKLVDVAEDNNLDYAVDV
                     FTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIENTLKLLINYMLKE"
BASE COUNT         3409 a         1331 c         1372 g         3515 t
ORIGIN      
        1 tcttttaact gtagttatag ttttttctac attaagattg ttcgttattc aataaaatgt
       61 atttgtatga aaatttatta aagtctacac atttaagtgt aggctttttt gattttttgt
      121 ttgaaaatta aggaaatttt aaggaaattt taaaatcgtc ttaaatattt attgatatac
      181 ttaaaatatt aatagcgaaa ggagacttat cagtgataga aagtaaaaag tcagaaaaat
      241 tgttactgtc aattatatac ataatatttt tggtatatat tttaatgatt ataagaataa
      301 ttttatttag agatgtacca atttacgcta ttttcaaggg aacgtttagg aatgtaaatt
      361 taattccatt ttatacaata tatcaattta tagtagatag taatcttgac tttatgaaag
      421 ctactgtaaa tattatagaa aatataggta tatttattcc tatgggaatt tttttaccta
      481 tagtatgtaa aaatctaaat aaaaaaacta taattatagt aattatactt gttagcctag
      541 catttgaatt aacacaatat atttttgctc ttggaagtag tgatatagat gatgttattt
      601 taaattcatt aggtggaata attggaatta atatttacat aaacatgaat aaattatttc
      661 ctaatgatat taaaagattt aaggctataa tagctactag tttagtttta ggagttttag
      721 gattaggagt tatttctaaa aattatcata acttactaac ttttaaattt agacctgata
      781 gaaaaatatc aaaaatatta attgaagaaa atagggaaat aattaaagat ataaataaag
      841 atacagtaga tatagttggg acatttgaga gctttaaaaa aggaataata actattaaag
      901 caggttctaa taacaaagta aagagaccag agaatttaat tgattctgat ggaaatataa
      961 gaatttattt aaatgaaaat acaaaattag tatcttatat aattaccaaa gaatcaaaaa
     1021 tggatatagt taaatatgag gaatttgatg tgaagaattt agatttatta aggagatatg
     1081 atactattaa tgtttggatt gataaagaaa atcagccaaa ggataaacat ggtattatgg
     1141 ctagtaaatt attaattgga ttgtatgaat aaaatattaa acaagaataa aagaataaca
     1201 aatataaaaa acgaaaaaac tatttctata attattttta cgttgtgcat aaaataagaa
     1261 cattagtgaa ggagtagaga taaattatgg agataataaa atatatcttg tactttttta
     1321 tatattcatt tttaggatgg acagtagaaa gtataggatg ttctatagca agcaaaagaa
     1381 taatcaacag aggtttttta aatggtccaa tatgccctgt ttatggtttt ggagcagtaa
     1441 ttgtgatatc actattagga agatttaata atgtagtaat agtatttcta ttaggaatga
     1501 ttttaactac gatactagaa tattttacag gatttatttt agaaacctta tttcatgcta
     1561 aatggtggga ttattctgac agaaaattca atataaaggg tagagtatgt ttaaaaaatg
     1621 caatatactt tggtgtaatg tctgttttaa taataagatt tatacatcca tttataaaat
     1681 attttgtaag tataattcca tatagaatat taatttcaat ggctataatt acaacacttt
     1741 ggactatact tgatttgatt gttactatta taacacttaa aaaattagat ataaagttaa
     1801 acttactaga cgatataatt acagacctta atgatataaa tgtgaaattg gataaatttg
     1861 atagaggaga aatacaagct ttatttaaaa caataaataa cgacggatta gaagttagag
     1921 aaaaaataaa caaaataaat agtaagttgg accgtataga gtctaatatt attttacaaa
     1981 aaagagttat aaaagcattt ccacatataa aacataaaaa acaacaagag caactagaac
     2041 attttaaaaa aattattaat gagaaaaggt aattatacaa tttaatgaag acgaatagaa
     2101 taatggtatt tataagatta ttttcagagg actctataaa aatagaatag agtcctttat
     2161 tttttgaaaa ataatgttta tttgataaaa ataaaatata ttgtttctaa ataacaaata
     2221 ttttgaaaat tataaaaaca tatattgaca atttgtgtca aaaatactaa cattaagtat
     2281 ataaagaatt ttatatgaga atatctttat aaatcgcaat aattttagaa cataaattta
     2341 tttaaagagg ggatgatttg ccaatgagcg agataactag tcaaatgata agtacagaag
     2401 aaaaaaaata tgttgctaaa actcaaaaga taccttatta tccagtagca tttaaatcag
     2461 gtgatggtgc aatgctatat gattatgaag gtaatgaata tgtagatttt ttagcaagtg
     2521 caggctctgc aaatgtaggt catggtaata aggaaatctc acaagcagtt aaagaacaaa
     2581 tggatgatat aacacaatat acacttgcat atttccatag tgatcctcct gtaaaattag
     2641 ctgagaagct tgtggaaata gcccctggag ataatgataa aaaagtacta tacagtgcaa
     2701 ctggttctgc ttgtatagat gctgctataa aattagcaag agggtatact ggaagaacaa
     2761 agataatatc tatgtgtgaa tcttatcatg gaagtactta tggagctata tctatatctg
     2821 ctttaagtac aaatatgaga agaaaaatgg gtccattgtt accagaagtt tatcacttcc
     2881 attatcctga taaaaataga acagctaaag aatgtttaga tgagatagaa tatgcttttg
     2941 cacactatct accagcagaa gaggtagctg caatatttat agagccaatt gcaggagatg
     3001 caggaataat agtgcctcca gtagagtggg tacaaggttt aagtaaaata tgtaaagaaa
     3061 atggaatatt acttgtaagt gatgaaatac aacaaggtat gggaagaaca ggtaagtggt
     3121 ttggtataga aaattttggt gttgaagcag accttatagt tcttggaaaa tctgttggag
     3181 gtggattacc tttaggagca gttgttggaa gaacagaaat aatgcaaagt cttgatgcac
     3241 cagcacattt atttacttta gctggaaata caacagtgtg tgtcgctgct ttaaaatcta
     3301 tagagataat tgaaaaagaa aacttacttc aaaagagtat agaaatggga gattatatta
     3361 aagcaggatt tgaaaaacta aaagaaaaat acgatataat tggagaaata agaggaatag
     3421 ggttatctat aggagttgat atagttaaag gaaaaggttc taatgaaaaa catccagatg
     3481 ctacagctaa gatatgttat agatgtatac aaactggatt aataatgata ttcttaggac
     3541 aatctacttt aagagtacaa ccacctctag taataactaa ggagcaagta gataaagcaa
     3601 tgagcatcat agattctgca atagatgatt atttaaatgg tagaattggt gatgaagtat
     3661 atgaagtaac tcaaggttgg taaaataaat attaaatgaa aaaaagagtt atctcaattt
     3721 tagaaagtaa tttctgaaat gagataactc ttttaagtta cccacaaata agaaaattgt
     3781 ttgattcatt aaagactata tttcggatgt cgtttttgtg agtatcagag acagtattac
     3841 gagaataatt acctttattc gtactatctg ttaatttagt tataattaag atagctaaaa
     3901 gaataagaat tactgtgtat aaagcaataa catatggttt tcttttatat ttgtatttgt
     3961 tattgaaaaa agaatggcca taattatttt tatttacttt agatgtattt gtattctttt
     4021 tcttgttacc tttcttttta tcatcctttt ttctattttt agggtaagga atgactttgt
     4081 gtgtattatc aagtttgata tttttatttt tttgaatatt gttttccagg ctagcctttt
     4141 tttctccaaa ttgaatagtt ttattggtat tgttgttatc tttcaatgtt aatctccctc
     4201 ccttaaactt tccctttaga taatattact actaacctat ggaatataca ataaataata
     4261 tataagtaat aaaattttat aggtataaat ctctttctcc acgctctaag gcagtcatat
     4321 tgttagatac tatgtttctt atatcttctc tttctatctt acctatttct tctgcgataa
     4381 gttttttacc cataatttta agttcttcat ctccataatc gattaaaaat tcatttaatg
     4441 tagtaagagc atttggcgta catacattat ggatttgtcg acttttagct aagctcatga
     4501 atctatcgcc agttctacct ttacgataac aagctgtaca ataactaggt atatatccat
     4561 cagtaatcag ttctttaagc acctcaacag ggcttctatg gtctccaact tcgaattgtt
     4621 ctgtattgtc accatcttca tatgctttat atcctccaac acctgttaga gaacctgcac
     4681 taatttgtga tacaccatat tttagtaact catttctcat ttcagcagtt tctctagtgg
     4741 acattatgat tccagtaaaa ggaacagcca atctagttat agctactatt tttttgaaca
     4801 tatcgtcaga aactaagtga ggaaaatctt ctaagctcat accttcagct tttttcaatc
     4861 tagggaaaga tatagtatgg aaacctacac caaatttttc ctctaagtgt tcgttgtgca
     4921 tcataagtcc tagaacttca aatttagggt cagataaacc aaataaaact cctgcaccaa
     4981 catcatcaac accagcttcc atagctctat caaaagctgt taaatggtaa taatagtcat
     5041 tttttataga ttgaccatgc atttttataa atgttggttt gtgataagtt tcttggaata
     5101 atatataagt tcctattcct ttttctttta gtaatttata ctcatctaca cttgtagctg
     5161 ctatgttaac gtttaccctt cttatatttc cgttttcatt atatgttgaa taaattgcat
     5221 ctaagcaatc taagatgtaa tttatatcac aatttttagg gtcttcacca gcttctagag
     5281 caagtctttt atgacccatt ttttctaata ttttaacctc ttcctttatc tcttccatgg
     5341 taagtttttt tcttttaaat ttgttgcatt tactaaaacc gcaataaaca cattcattta
     5401 cacagtagtt tgatacataa agaggggcaa ataaaaccac tctattacca tatatttcat
     5461 tttttatttg cccagctata ctaaataatc tatctagttg ttttttatct tctacttcaa
     5521 gaagtgtagc tatatcttta taactaagtt tttcccttct atcagcctta tcaagtacct
     5581 tttcaatatc atcagaagtt gaattttttg catcttctaa taaagaatta ataagttcat
     5641 ggtttatgaa catttacaaa cgcctccatt caatattatc accgcgagaa tagtcaactt
     5701 caagtcctaa tgacttgatg ttttcttcta aactttggtg atattcacaa gcttcttgac
     5761 cagtaaaggc tttgttatcg taaagagaat attgttttct aaattcttga ggagaaaggt
     5821 ttggcataat tacattgcaa ccagctaaaa gacctgcatt tctaccacta gggtttatag
     5881 atgccaaggc tgttgtagcg ggtaataaaa cttttggaag tagaagtctt gtaattgaaa
     5941 gcataagtaa tgtcttctct aaatctccat gtttataatc cttaaaaatt gtatcatggt
     6001 gtggaataaa aggtcctatt ccaaccatat gaggatttaa ctcttttagg tataataaat
     6061 cacgaacaag gtctttgttg ttctgaaatg gtgattcaac cataaatcca gcacctacct
     6121 gataaccaat ttctttaagg ttttttaggc attcttttct agttctaagc tcaatgtttg
     6181 gcggatgtaa ttttttataa tgactgtctg tagcagtttc atgtcttaaa agatatctat
     6241 ctgcaccaca taaaaagtat tttttatacg aatcataact cttttctcct agtgacagag
     6301 ttatggcaca atcctcatat ttatttttta tacttgaaac aatttcacat attttatcat
     6361 cagtaaaata tgcatcttca ccaccttgca aaacgaatgt tctgtatcct attttataac
     6421 ctatatcaca acattcaagt atttcatcag gtgaaagtct gtatcttatg atatttttat
     6481 tagaagattg tattccacaa tagtagcaat tatttttaca atagtttgta agctctataa
     6541 gtcctctcaa gtagactcta tttccataat gtttatttct catgtcgcta gcttgtttat
     6601 aaagatagtt tcttaaattt tgttcgaagt tatattcctc attgtcgata ttttctatta
     6661 aatatagtag ttcatcaaaa cttaatgaat taatgctgta aagtttatct ataaagtatt
     6721 taatcttatt tttagtcata ttcaaatatg taattattaa gtatagttaa atttttaata
     6781 attgaaaagt tcaagtgatc tttccaaaat accttttaca tatgctatta agataccata
     6841 gtttacaata ggtacattga aagattttgc cttttctatt ctagataaca ttccagctct
     6901 attcatcata caagctccac agtgaactac aagggcatat ttatttatat cttcagtgaa
     6961 ggatactcca gaactaaatt caaaagtaat atttttgcca gttttctttc taatcatatt
     7021 aggaattttt actgttccaa tatcatcagt ttgtctatga tgggtacatc cttcagccat
     7081 caagatttta tcaccatctt ttaaattttc taaagcataa gctccattaa tcaattcttt
     7141 aaggtctccc ttttgtctag caaacaatat agaaaaagat gtaagaggaa tatcttttgg
     7201 tgtatcttta tttacttgtg gaaatacttg agagtcagta ataacaagtt ttggtttttt
     7261 gcctaaatta gatagagttt cttttaaact atcttctttt gtaactatag aaattgcacc
     7321 attatccaaa atatctctta taacttgctg ttgaggaagt ataagtctac cttttggagc
     7381 tgctttatct attggaacaa ctaaaacaac taaatcattt ggttcaatta aatctgatac
     7441 caatttgaac tcagtgctat ccttaggcaa aacttttatt atttcatttt ttaaattttc
     7501 aattccaatt ttatctgttg atgagacaga aacaactgga catttaactt tatttttagt
     7561 taagtttaaa atttctgatt gattcttaat agtatcaatt ttgttgagaa ttagaatatg
     7621 agggatattc ttatcattaa acttttcaat tagtgacaaa tcttcttgtg atataccaat
     7681 ttggcaatcc actactagta aagctatatc tgtcttttct aaaacatcta gtgatttacc
     7741 tattctaagt tcaccaagtt caccaacatc atcaagacca gcagtatcta taagaacaca
     7801 aggtcctata ggaaggattt ccattggtct aaatactggg tctgtagtag ttccagcaat
     7861 atctgagact attgcagcac tttgatttgt tatagcattt attatgctag atttacctgc
     7921 atttctttta ccaaagagac ctatatgtac tcttaccgat tgtggagtag aatttagact
     7981 catttgtact caccacctta aacttctttt tttgatatac tagtttttac agatacatga
     8041 ggtatgttgc ccaatttacc agtcaaacta ttaatagtat ccatttcgcc aacaactgct
     8101 atacatacga ttgagacacc ttcttcttcg caaggaatgc ccattcttcc tttaataata
     8161 cctttaaaac ctgatacaac ctcattgaat ttaaattgac attcttttgg ctcttccaaa
     8221 atagcactta taactgcaac ctttttcata aatatcctcc taactcagaa aatatgtctt
     8281 aaacaaatcc aaaatcaaaa aacttttctt tgtaaagaaa agtttctaca aaagaaaagt
     8341 tattaattac acaagtataa tgtgctatat taaactattt ccttacagat tagataactc
     8401 ttttctggta gggttagctt gtaaaaattt aatttaacaa caagtaactg aagaaaattt
     8461 ttatacaatt tatataaatt ataataaaaa ttaactaaag aaaattgaag ttacttatat
     8521 aatagtagta aattgaaatt aagtctatag aaaaaccaat ttttgtataa aaataaaaat
     8581 taaagttaat tatttgttaa gaattgactt tggtaaaata tataattaat tattctttaa
     8641 gcatataatt tataagtaat tttagtgtat tttcaatagc ttctatatgt gttctttcat
     8701 aatggtgact attgtttgtt ccaggaccaa cacatgctat ttgaacatct tctccccaac
     8761 gaatagcttc tgaagcatct gaaccataga aagtaaatac atcgacagcg tagtctaaat
     8821 tattgtcttc tgcaacatcg acaagcttgt ttcttaaatt aaaatcatat aatgataaat
     8881 tatcttttgc aactatagtt acaccatgtt cactagatgt ttgacttggt ccagtaggtg
     8941 ctatatctat tgctacaaat tcttttgttt tttcaggtaa acatttagaa acaccatgtc
     9001 caatttcttc ataattactt atataaaagt tagtagtatg cttaggcgtt aaattgtttt
     9061 ctttaaaata tctacatatt tcaagaacca ttgctacagc tgatttatta tctatatatc
     9121 tagattttat aaaaccagat tctgtagcct caaatctatg gtctaggcat acaaaatccc
     9181 caacacttat tcctaaatct aatacatctt gctttgtaaa tactttttca tcaagtctaa
     9241 taatcattgt ttcttcattt ctttcagtgg cagcttttgt ttgaccatat atatgagtag
     9301 aagcatactt aaatacaaca cttcctctga tttttttacc ttttctagtt ataacagtgc
     9361 agttttctcc ctctatagct gcccaacatc caccaccaac tttatggtat ttaagagtac
     9421 catcagatga tatttctttt actatagctc caagtgtatc catatgtgca gatattgtta
     9481 tttgatttga atcatcttca ccaggaagag tagctataag tgcacctttt tttgtaagaa
     9541 tagtgtcaag acctaaattt tcaaaatctt ttttgatttc taaaactgca ttttcagtat
     9601 atccagctgg gcttggaatg tccaaat
//
LOCUS       DAELSY010000148         9525 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.148, whole genome shotgun sequence.
ACCESSION   DAELSY010000148 DAELSY010000000
VERSION     DAELSY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.148"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            496..1701
                     /locus_tag="KPG09_003135"
     CDS             496..1701
                     /locus_tag="KPG09_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG0967721.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVGPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNVKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1772..1993)
                     /locus_tag="KPG09_003136"
     CDS             complement(1772..1993)
                     /locus_tag="KPG09_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967722.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(2134..2733)
                     /gene="recR"
                     /locus_tag="KPG09_003137"
     CDS             complement(2134..2733)
                     /gene="recR"
                     /locus_tag="KPG09_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBG0967723.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2746..3096)
                     /locus_tag="KPG09_003138"
     CDS             complement(2746..3096)
                     /locus_tag="KPG09_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBG0967724.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3155..4792)
                     /gene="dnaX"
                     /locus_tag="KPG09_003139"
     CDS             complement(3155..4792)
                     /gene="dnaX"
                     /locus_tag="KPG09_003139"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBG0967725.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKYSIDENESK"
     gene            complement(4869..5133)
                     /gene="ffs"
                     /locus_tag="KPG09_003140"
     ncRNA           complement(4869..5133)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KPG09_003140"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5171..5262)
                     /locus_tag="KPG09_003141"
     tRNA            complement(5171..5262)
                     /locus_tag="KPG09_003141"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5226..5228),aa:Ser,seq:gga)
     gene            complement(5263..5718)
                     /locus_tag="KPG09_003142"
     CDS             complement(5263..5718)
                     /locus_tag="KPG09_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBG0967726.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(6038..7309)
                     /gene="serS"
                     /locus_tag="KPG09_003143"
     CDS             complement(6038..7309)
                     /gene="serS"
                     /locus_tag="KPG09_003143"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG0967727.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKTRLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEYKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7434..7640)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7767..8648)
                     /locus_tag="KPG09_003144"
     CDS             complement(7767..8648)
                     /locus_tag="KPG09_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG0967728.1"
                     /translation="MNFLSLAKAASTNSNITKRTLENMLNNFSDNIPNIIYAIIVFVI
                     GIYISKIVRKMVSRFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGVIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFINEKR
                     ILDDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENNIKVPFVP
                     SSLLFNNMSNSNTMDKR"
     gene            complement(8660..9169)
                     /locus_tag="KPG09_003145"
     CDS             complement(8660..9169)
                     /locus_tag="KPG09_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBG0967729.1"
                     /translation="MNSDERRSKLIDILKESKCPVKGGMLAEILNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHRNSKEIYDELKIIVDLGGIVKDVIVNH
                     PTYGQITAELNIHSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIETLKEETIEVITK
                     ELEDRGILS"
     gene            complement(9296..9412)
                     /gene="rrf"
                     /locus_tag="KPG09_003146"
     rRNA            complement(9296..9412)
                     /gene="rrf"
                     /locus_tag="KPG09_003146"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3036 a         1667 c         1026 g         3796 t
ORIGIN      
        1 ctcagatact attgttatca aaatatctgg ctttatggtt tgtttcttaa tataaattaa
       61 cctactgttt aattttcaat gttcgtgttt atttcttttt ccttttttca aggacaagta
      121 atactatatc atctttatct attctagtca atataatttt ttactttttt taaatttttt
      181 atttatcatg tattctttat atttaatttt taaaacttaa tgctaagtaa acctatttat
      241 ttcataaaac taatatatat tctatgctct attttccttt ttattctata tttaaatttt
      301 ttatttacaa gaatattttt aatataacat atttgataaa attaatttac atttctttca
      361 cttgtaattt ttgcaatctt gtattagatt aatattaaaa ataatttatc aataacctta
      421 ttcacaaaga cagtttttat atatcaaaat ataatacaat ataaatacta tgctacattt
      481 taggaggatt ttattatgag caaaaaagta ttaataatgt ctgcttccac tggtggtggg
      541 cataataggg ctgctctagc tattaaagaa gaacttactt ccaaaacact tgatggagag
      601 ccaattgaat gtgaaataat agatagtttg aaattagtta ataatacaat ggataaaata
      661 atttcaagag gatacgaaaa atctgctata tatactccca aagcctatgg aagtgtatat
      721 agattatctg aaacaaatct tttgtcaaaa aatgaattta aagataatct actaataaca
      781 ttcatggcta aaaaatttaa aaagcttatt agaagcgaaa aaccagactt gattatagga
      841 acacatcctt ttccaatgat tgctctaagc acattgaaaa aaaactttaa tttacataat
      901 aatgaatcaa atgcttacac agagcatttt tataaacatt atacaaatac cattaatgtt
      961 cctccgctta tttctgtatt aaccgattat actactcatt ctacatggat acaaaatgaa
     1021 attgattact atattgttgg tcatgaatat gtaaaagaat tattggtgtt tgatggagtt
     1081 ggaccgagta aaattagaac atttggaatc cctgttgaaa aatcttttct ctctcacaga
     1141 gataaagata ttgttctttc tgagttgaat ttatctcctg ataaattaac tgttttactt
     1201 atggggggta gctttggggc tggaaatata aaagagacac ttgatgaatt actggatact
     1261 gatagagatt ttcaaatact tgtaattact ggtaagaatg aaagcctaaa agaaaaaatc
     1321 gaaaaaaaac ttatgtctcg ttatcatgat aaaaatgtat gtgttcttgg atacactgat
     1381 aagatgaatg atattttagc ttctatagat gtattagtaa gcaaacctgg tggacttact
     1441 acaactgagg ctcttttaaa agatgtacct atgatagttc cttattatat accaggtcag
     1501 gaagaagaga atcttgattt cttatctaat tgtggtgcag cacttagaac tacaaaaaaa
     1561 tataacttgt caatattact aaaagttcta atagatgacc ctagcagatt agaaatgcta
     1621 aaaagaaatg ttaaatcaat aagaaagtca aattcagccc aaaatatagc taatttaatt
     1681 ttaaatataa tttctggttg ataaaataaa taaaatccct atagtatata tattatctat
     1741 tagggatttt ttattttcta attatttaat attaaattgg ttcataaaag tcttcttcat
     1801 aatcatactg ataattttta taattatttc taaattcagc atttttattt cgtacatttt
     1861 ttttagtttt tttcttatta gaaaaactta tatcctcaaa atcatcatat ttaggtttat
     1921 cttttgattt cattttttta actctttttt ctttcattgc attatgttct tctaagttca
     1981 taattggttt catgttgcac ccctatcaaa atttttaaat gatttataca acaaattata
     2041 caatacagaa gaaatttatt caataaatct aaatcaaaat taatttgaat ttaactcaaa
     2101 aaactacaaa aatcgaggta ttaacctcga tttttatatt tctcttctgc cttctagtgc
     2161 ttttgatatt gtgacctcat ctgcatactc taaatcgcct ccaacaggaa gaccatgagc
     2221 tatccgagtg actttgattc ccattggttt tactaaccta gcaatataca tggctgtcgc
     2281 ttcaccttca atagtaggat ttgttgccat tattatttct ttgacttcct gattgccaag
     2341 tctttggata agttccttta tttttaacat atctggtcca attccatcca ttggagaaat
     2401 tactccattt aatacatgat actgtccttt aaactctctc gttctctcca tagctgctac
     2461 atctcttgga tcttccacaa cacatataac acttgaatct ctacttttat ttgaacacat
     2521 gctacatggg tctgtatctg ttatattaca acaaattgag caatatctta tttctctttt
     2581 tgcatctata atcgctttag ataaagcttc cacatcatta gtattcatat taataatatg
     2641 aaaagccaat ctttgagcag tctttcttcc tactccagga agctttgaaa attcctctat
     2701 aagtctcgtt atgggacctg tataaacttg cataaatacc acctactaga ataacccagg
     2761 tatattcata cctccagtta ccttactcat ttgacttgct tgtatatcat caatatttct
     2821 caatgcttga tttactgcac ttaaaactaa atcttgtagc atttctatat catctggatc
     2881 aactacttct ggcttgatag ttatatctat aacttctttt ttaccattaa cttttacagt
     2941 aactgctcct ccaccaactg aagcttctac ttctttactt tctaactctt gttgagcttt
     3001 ttgcatattt tcttgcattt tttgagcctg ctttaacaag ttgttcatat ttccaccacc
     3061 aggcatcatt ccgcctccaa aacctttttt agccataaac actattcctc ctaaacttat
     3121 tttcatttta ttattatatc ataatagtat tttattattt actctcattt tcatcaatac
     3181 tatattttat ttctaatatc tcttcactca ctatgttctt taatatctcc tcaccaatgt
     3241 cctttttctc tttaacttca agatttatat ctttaacttc tgatgtcagt actattttta
     3301 cattaaaact tctatttaaa acatctctta ttgttttttc taaataattt attgtatctt
     3361 gagagctcag tctagttttt gcaaaatcaa atttatcatc aaatataaca tacaacatat
     3421 tagaatatac attaaaactt tttacttcac ttaagagtgc gtatattggc atttttttat
     3481 cttttttaat ttgtttaagt atattcttcc aagatgactc tataagtata acgtcctcat
     3541 tttttacatc ttcataaact acattttctt gttttacatc tatttctcta tcgacctcat
     3601 actctttttc tttattattc ccaatttttt cagacttaaa gttccccagt tcaatcattt
     3661 tttctaagtt ctcaactctt tttataagag cttctttact ttcatcaaac attggttgag
     3721 caattttcat tatggttatt tccactaaaa cccttggatt tgaagatgac tttatatcat
     3781 cctgagttat tgataatata tttaaaatcc ttattaaatc atttatatct atatttttag
     3841 actgaatttt aagttgttct atagtttctt ctggtaatga aattatttca tccaattctg
     3901 atgaaacctt acataccatt aaatttctaa aatgatcaat taagtcattt ataagatttc
     3961 ttatatcttt gccccataag acaaattcat ttaatatctc aagagacttt ttagtgtcct
     4021 gctctacaat acattgagac aattcaaata attgttctat atttacgctt cccattaatt
     4081 caacaacatc tttataatct atcttacttt ctccaaaaga catacattgg tctaatatac
     4141 taagtgcatc tctaagagct ccttgagaat ttctagctat taaattaaga gctttatcat
     4201 ctatatcaat tccttcatct tcacaaatct ttttcattct aagggtcata tctttaactg
     4261 taactctttt aaaatcgtat ctttggcatc ttgataaaat ggttgcaggt attttgtgag
     4321 gctctgtagt tgctaatata aaaattacat atgatggagg ttcctctaag gtctttaata
     4381 atgcattaaa agctccttga gacaacatat gaacttcatc tattatatat actttatatt
     4441 tagcattggc tggagaatat ttcacacttt ctctaagctc tcttatatca tctacactat
     4501 tatttgaagc tgcatctatt tctacaacat ccattgtatt atcttttaat acagattcac
     4561 atatctcaca ctcattacac ggttcttcat ttattttatt caaacagttt actgctcttg
     4621 aaaaaatctt tgcagtagat gttttaccag tacctcttgt tccacaaaac aaataagcat
     4681 gtcctatatt atcactatat atttgatttt ttaaagtctt aataatatga tcttgaccaa
     4741 taacatcttc aaatttttgt ggtctatatg ctctatataa tgctttatgc atctttttac
     4801 ctctctttag caaaattaat aaaaaaatcc ctcattatct aagggattag tcttttatat
     4861 attaaatgct gtacaccatc tatcgactta attccatagg cgttaccaaa acagttagct
     4921 caacttaggc acccccacgg cacacaaaag tgatcactta ccgctgcttc cttccggatc
     4981 tgacggggtt catgagcttt cattgcgcag gacctaagca tcaacaccac ttatttaggg
     5041 cggccctaca gaaaataagc ctttagatgg aattcaatcc tgctatagcg gattgcaggt
     5101 tacagggcac cgctacctcc ccatctagta cagcaaaatt acaaccatat ttaattactt
     5161 tcattgtttc tggcggagag agtgggattt gaacccacga tacagtttta cccatatact
     5221 cgctttccag gcgagcgcct tcgaccactc gaccatctct ccttaatctc tttttctcaa
     5281 tgccttaaaa aattcttgca atatgatcga gcattcttct tgcaaaacat caaaagctac
     5341 ttttacatta ttattattaa aataatctaa tttaaactca tgctgttttt ctatatatga
     5401 attttttaca tgtcttgtcc ctattataag cttttttatt ctagaattaa ctattgcgcc
     5461 actacacatt atgcaaggtt ccattgtaac atataaatca caatcagtca acctccaacc
     5521 acctaatttt tctgatgctt gttttatcgc taaaatttct gcatgtgccg tagagtcttt
     5581 taacgtttct gtaaggttat gagctctagc tattatttga ttatctttaa ctataatagc
     5641 acctattggt gtttcttttt tattatatgc tttatatgct tcttttaatg cttctttcat
     5701 ataaaatgaa ctttccataa ttcctcccaa aacttataac aaacttatct tatcataatt
     5761 cattttataa ttcaacttaa ttttattttg tttatttaat tattactata tatagcgata
     5821 ttttttttaa ttagttctaa aataattttt agatattcat acaagagtgt tttataaaaa
     5881 tatttagtgt tttatgatga aaattctgct gtgtaaatta acataaaaaa actatgaaat
     5941 taatatttat aataatttca tagttttttt atttattgtt tactcataat atattctctt
     6001 catcaaagca tactctacat atacaattaa tataaattta ttttataaca tcaacaccca
     6061 tataaggtct taattcttct ggaactacta cagaaccatc tgcctgctga taattttcta
     6121 atatagcagc caaacatctt cctatcgcta atccagaacc atttaatgta tgaacatatt
     6181 ctgcttttga ttttttatct cttttaaatc ttattcctgc tcttcttgct tggaaatctt
     6241 caaaatttga acaagaagat atttctacat atctattgta acttggcatc caaacttcta
     6301 aatcatattt aaatgcagct gtaaatccca aatctcctgt acatattttt acaactctat
     6361 atggaagacc taacatttgt aacatagttt cagcatcatg agttaatgat tctaattcat
     6421 tataagaatc ttctggttta acaaatttaa ctaattcaac tttattaaat tgatgttgtc
     6481 taactaaacc tcttgtatct cttcctgctg aacctgcttc agatctaaaa caaggagtat
     6541 atgcacagta tttaattgga agctctgcaa catctaatat ttcattagca tgtatattag
     6601 taacaggaac ttcagctgtt ggtattaaga aatactctaa tccttctatt ttaaacatat
     6661 cttcttcaaa tttaggaagt tgaccagtcc ctataaaact atttctattg gccataaatg
     6721 gaggtaagac ttctgtatag ccatgcttag ctgtatgagt atttaaaaag aaattcatta
     6781 aagatctttc taatctagct cctaatcccc tatataaagt aaaccttgac ccagttattt
     6841 tccctgctct ttcaaaatct aaaataccta gacctgtccc tatatcccaa tgcgctttat
     6901 actcaaaatc aaatttagta ggttctcccc aagttcttat ttgaacattg tcttcatctg
     6961 tttcaccttg tggtacctct ggatgaggaa catttggtat tctcattaat gtatattcca
     7021 tttttgcttc tacttctttt actttctcat caataccttt tatcttatct gataactctt
     7081 tcaatcttgt cttttcttct gttacatctt ttccttcttt tattaattga ggtatttttt
     7141 tagaatttac atttaactca tttttcataa cttcaacttc ttgaagtatt tctcttcttt
     7201 tattatctag ttccactaca gcatcaaggt caaattctct ttcacctctt ctttccatag
     7261 cttttttgat atcatctaga ttttctctta ttcttttaat atctaacata ttaattcctc
     7321 ctaataattt tgataatata aattataatt ccaattaatt tacaagtatt ctagctgtaa
     7381 acttttaatt taaacaaatt tgaaaaataa aaaaagacct ccgtttaccc tataaaaggg
     7441 acgaaaatct tttatccgcg ttgccaccct aattgattat actaaaacgt ataatcctct
     7501 cagacctata acgtaggtaa accgtctagc tttaactaga agctctagag tagattcaaa
     7561 aaccattgta atgagttctc accatccact cattctctga atacaatctg ttttttacta
     7621 ttttccttca ccgcttttta actaattgtt ttatttgata atcattatat tatatatatt
     7681 aacatctttc aatactatta ttcaaatttt tttagaattt tctttaattt tttatattaa
     7741 attcactttt ttacttaatt gaaattctat cttttatcca ttgtatttga attgctcata
     7801 ttattaaata ataacgagga aggaacaaat ggaactttta tattattttc gtcaaattta
     7861 tttttgactt tttccattac atcaaagtaa acatcccagt aatcatcagt tttaacccat
     7921 ggtctagcaa ctatttgcat agtgttgttt cctatagagt ttaaacctat aattggtttt
     7981 gggtcatcca atatcctttt ttcatttata aaaatttcat tcaatatgga cttaactaca
     8041 tcaatatcac tatcatatga aatattatat gaaaaatcta tccttctttt attttgatgt
     8101 gtgtaattaa ttatattgtt acttgtcaat tgaaagtttg gtataattat ggttttatta
     8161 tctggggttt taagaacagt actaaatatt tgtatatcag aaactgtgcc ttctacacct
     8221 gagccttgaa tataatcacc tatattaaat ggcttaaaaa ataaaataat catacctgaa
     8281 cctaaatttg ataatatttc tttaaatgct aaacctatac taaatccagc agctcctaat
     8341 acagctacaa ctgatgtagt ttgtatccct ataacaccca agcttatcaa tgatataata
     8401 gataacatag atatgtatat tccatataca atgaaattat ttacaccttt actcattcca
     8461 tatttgatta aaaaccttga aaccatcttt cttactatct tagatatata gatacctatt
     8521 acaaatacga ttattgcata tataatattt ggtatattat cactaaagtt attaagcata
     8581 ttttctaaag ttcttttagt tatattacta tttgttgaag cagccttagc taatgataaa
     8641 aaattcattt aactcctcct tatgataata ttcctctgtc ctctaattct tttgttataa
     8701 cttctatagt ctcttctttt aatgtctcaa ttgtatgaat atgactacat tcagttagaa
     8761 ctgataactg tttaaattca tttgtctcaa ccttcttcat aaaattatct atatccattt
     8821 tagagtgaat attaagctct gcagttatct gaccatatgt tggatgattt acgatgacat
     8881 ccttaacaat tccacctaaa tctacaataa tctttaattc atcataaatt tctttagaat
     8941 tcctgtgatt cttacatttt atttgctttg taaaatatgg ttgattatat attatatatc
     9001 cttgaggtgt agctattatt tcaaatcctc tagctcttat aagggctata tcttgtacta
     9061 ttacttgcct acttacattt aatatctcag ctagcattcc accttttaca gggcacttag
     9121 attcttttaa aatatctatt aatttgcttc ttctttcatc agaattcata ttacaccttc
     9181 tttctaaatc aaaaacttta cctatactat ataattctat attttatctt attataaatc
     9241 aatattgatt ttatattcta tttaatatta aactttattg caaataaaaa agattacgtg
     9301 gctacgtcct actctcccag gcagtttccc accaagtacc atcagcgcta gagagcttaa
     9361 cttctgtgtt cggaatggga acaggtgtat cctctctgct attgtaacca cataatcttt
     9421 atgcttaata tttctattaa gtttttagaa tctagtaata ctagtttctt tagagttaac
     9481 actctaaaaa ctacatatat attttattga atggacatca aataa
//
LOCUS       DAELSY010000149         9232 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.149, whole genome shotgun sequence.
ACCESSION   DAELSY010000149 DAELSY010000000
VERSION     DAELSY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.149"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(90..743)
                     /locus_tag="KPG09_003147"
     CDS             complement(90..743)
                     /locus_tag="KPG09_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0967730.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKFMRENDLENVERVLREHWKTIPIAEMKKEKLL"
     gene            complement(988..2853)
                     /locus_tag="KPG09_003148"
     CDS             complement(988..2853)
                     /locus_tag="KPG09_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HBG0967731.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDIPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVDENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(2858..5278)
                     /locus_tag="KPG09_003149"
     CDS             complement(2858..5278)
                     /locus_tag="KPG09_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBG0967732.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNIDNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYEQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(5292..6734)
                     /gene="glgA"
                     /locus_tag="KPG09_003150"
     CDS             complement(5292..6734)
                     /gene="glgA"
                     /locus_tag="KPG09_003150"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBG0967733.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNRDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6763..7893)
                     /gene="glgD"
                     /locus_tag="KPG09_003151"
     CDS             complement(6763..7893)
                     /gene="glgD"
                     /locus_tag="KPG09_003151"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBG0967734.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGS
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(7895..9055)
                     /locus_tag="KPG09_003152"
     CDS             complement(7895..9055)
                     /locus_tag="KPG09_003152"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBG0967735.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKRSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT         2910 a         1707 c          902 g         3713 t
ORIGIN      
        1 tatttgtatt taaagttaaa tttattcttg tttttccttt aatatttaat agtaaaataa
       61 caaaattata aaaaaaataa agagaaactt tataaaagct tctctttttt catctctgct
      121 ataggtatcg ttttccaatg ttctctcaaa actctttcaa cattttctaa atcattctcc
      181 ctcataaatt ttactacttc aatatgttga ttattgctgt atgtcaacat tttatataac
      241 tcatcttcat tatcactagt atatgacttt gacataaaat tatatcttaa agagttcaat
      301 agatttacta acacctgatt atctgattta gttatgtata tatcgtgaaa ttcattactt
      361 aattttaaat agctattgta atttttatac tttatagaga ccgccatcat ttctgttaat
      421 tcttccattt ttactaggtc tgattcagtt aaattttgta atgctgaaga accggctaac
      481 gcatccaata ctccaattac ggaataggtg tctaatttct ccttttcctg aaattctttt
      541 actacaaatc ccttattagg tattttttct agtaaatttt cacctgctaa cctagttaat
      601 gcttctctag ctggtgttct actaactccg atagatttac aaatctcaga ttcagtaatt
      661 ctctgattag cttcatactc tccattttgg attttctttg atatatagtc atatacttga
      721 gttgtaagtg gatctacctt cataagacgt tcctccatat taagattata aaagttaaat
      781 ctccctacaa ctttggtaga gagttcagtt ttatcttact aatttatata atatcacaat
      841 tatgatattt tttatacttc tttgtaatat tttgaaacac taaaattctt atgataattt
      901 atacaaaaaa agtagaaaaa gttttgataa aacaaaactc tttctacatt caccttattt
      961 tctatttatt tttctaaaaa aagatattta atttactcta aaaattctaa atgatttaga
     1021 gtctaaatct actttcaact cattattttc aatttgtata gtactatttg catttttaag
     1081 actatacttt ataggtattt cctcaataaa actactctct atacataatg atatactttc
     1141 cacattttca cttctattta ctaaaattaa cattctatca ttattttcat caactctaat
     1201 aaaagaaaat acagaatcat tattagtata tataaattct gtatttccag aacttaaaag
     1261 tttattctta tttctaattt ttataacatc tctataaaaa ttaagcatat cttcgtcttc
     1321 atttttccat ggataagttc ttctattatc tgggtcaaca cctccacaaa gacctgcttc
     1381 atctccataa tatatatatg gtactccttc aaatgtcatc tgtatagcta cagcaagctt
     1441 cactaaatct acatcatcat ttaattccgt tttagctctt gtaacatcat gtgttccaat
     1501 taaatttaaa ttagacctaa atgattcttt tggataattt tctttaatct gcatatatct
     1561 attacaaagt tcatatgaat taatttctcc ttttaagaaa gaaaacatgt tatttctaaa
     1621 aggataaccc ataacagaat ctaattcttc acctaataaa taacttcttc tctgaccata
     1681 gctaatctta tttgatgcat cttcccatac ttcacctata agaattgagt catcatctgc
     1741 cttttttaat tcctttttta actctcttat aaacttagtt ggtaattcgt ctgcaacatc
     1801 tagtctccag cccttaatac ccatatttac ccatttattt ataacgctat cattgtcata
     1861 tattatataa tccatatagc tatgttctaa ttcatttata tttggcaatg tcttaacatc
     1921 ccaccaactt ttatactttt gaggaaattc ttcaaacata taccatgaat aatatggaga
     1981 ttctttagat tgatatgccc ctaaactatt atagtttcca tacatattaa aatatttact
     2041 atctgctcca gtatgactaa aaactccatc taatactatt gatatacctt tttcttttgc
     2101 tttatcaata agttctttaa atgtatcttc atccccaaac attgggtcaa tttttttata
     2161 gtcacctgtg tcatatttat gattactaga agcttcaaat atagggctta gatatagtat
     2221 acttacacct aattttttta aatatccaag tttattgatt atccctctta aatttccacc
     2281 atggaaatcc catcttataa catccccttg actatccttt atgtacatag gtatatcttc
     2341 ccaatttcca tatatgaaac tatttttttt aggattatca accttaccac tacgatttcc
     2401 attattgaat ctatctacaa atatatgata aagtattcct tctttgtacc atgttggtac
     2461 tttaaaatct ttgtaaacag tgatttgata tttattaata tcactataat tatattcaca
     2521 agaatatcca ttatctctgt tttttccata aaataaagtc tttatctgac catccatttc
     2581 aacatcaact ttaaagtaat aaaaataaac acctgtacca tcaaactttt caatcttacc
     2641 acaaaagcac tttccaagtt tatcattatc taattcttca tttaaagtta ttgttctcat
     2701 ttcttcttct ctatttattt ctagggaaat agacttgata ttgtatccct catttacttt
     2761 aacacaaatg gtcaattcct catcaaattt taaagctcca aacggagcct taaaattttt
     2821 atcccaagag ttatattcta ttacactttg catttaatca caccctttta aaccaaatgt
     2881 cttttgcata ctcagaaatt gttctatcag aagaaaaaaa tccagagtta gctatattaa
     2941 ctaaagacat tttattccat ttttctttat ctctatataa cctgtttata tctgcttgag
     3001 cttgaccata attttcaaaa tctctcaata caaaatactc atcattgtaa gtagtgagtg
     3061 aattataaat acttcttcct tcttcgccaa gattaggaat aaatccattt attaaatcat
     3121 ctaccaccct ttttatatcc atattagaat gatataagtc aagtgaagaa tatccaccat
     3181 atttattata atttagtact tgttctgcac taagtccaaa taaaaacata ttttctttac
     3241 caacctgttc acatatctct acatttgcgc cgtcaagtgt agccaaagtt atagcaccat
     3301 tcatcataaa cttcatatta cttgttccag atgcttcctt agttgtagtt gaaatttgct
     3361 cactaacatt tgctgctggt attattattt ctgctaatga tactccataa ttctctaaaa
     3421 atactacttt taacttgtcc tttactctaa catcattatt tatagtactt gcaacagaat
     3481 ttataaattt aattatacat ttagccaaat aatatcctgg tgctgctttt gcaccaaata
     3541 taaaagttct tgggtcaaga ttcaaattag gattatcaag taattcatga tacatatgaa
     3601 gtacattaaa tatattcagt aactgtcttt tatacgcatg caatctctta acttgaacat
     3661 caaatatcga acttgggtct acatttaaat cgtatttatc ttttataaac tttgctagat
     3721 ttgctttatt attgtatttt atatcatcaa gtctttgtaa aacagaggaa tcatttttaa
     3781 atttttcaat attttttaat tgtaaggtat ccttcttcca tgaatcacca attaaatctg
     3841 taattaaatt gctaagttga ggatttgaac ttataagcca tcgcctatgt gcaattccat
     3901 tagtcttatt attaaattta tttggttcat cttgatagaa atcttttaat acctctgttt
     3961 caagaatatg agtatgaagc ttagcaactc catttacact atgacttgtg acaatacata
     4021 agtttgccat attgatattg tcacagtcta ttatactcat tctttttatt ctatcctgct
     4081 catatccttt attatttaat tcctctacat atcttctatt tatttcttct attatcatat
     4141 aaattcttgg taatagttct ttcatcatac ttacaggcca tttttccatt gcttctgaca
     4201 ttatagtatg attagtatag gacactgttt ttttagttat ttcccatgct tcatcccaag
     4261 ataaattttc ttcatcaagc aaaattctca taagttctgg tatgcataag gtaggatgtg
     4321 tatcatttat atgaattgct accttatcga ataaattatc aatatttaat ttgttctttt
     4381 tatattttct tattatatct tgtatccctg cacttacaaa aaagtattct tgtttcaacc
     4441 tcaacaactt acctgcatag tttgaatcat caggatataa tacctgtgat atttcctcag
     4501 tataatactt atgttttaaa gcctcttcat agctaccaga ataatttaga gcatttgagg
     4561 taagttcacc aaaatctctc tttggtattt cactcttaaa caatcttaaa gtatttatac
     4621 attggttttg gtatccgatt attggtatat cataaggcat tgccattaca ggaaggtagt
     4681 ttttatgaat tacttttaaa tgactacctt ccttgattaa ttctacctca ccaccaaact
     4741 tcaccattgt tgcttcattt ggtcttacag tttcccatgc atatcgtcct tctgtaagcc
     4801 agttatcagg cacttctacc tgataaccat ttacaaattt ttgttcaaac agaccatatt
     4861 tataccttat tccatatcct tgaccactta tgttcaaaga tgccatagaa tctaaaaagc
     4921 aagctgctaa tctaccaagt ccaccatttc ctaaggctgg gtctacttca gcttcaatta
     4981 aatcatccaa gttgattcct aattcagata aaccttctct aacttcttct tcaatattta
     5041 aatttaataa attagatttt aactgtctcc ctattaaaaa ttctaaacta aaataataaa
     5101 cttctttttt cttatctaac tttgtagcaa cccatttttt tgctatctca tctttcaata
     5161 gactacataa tacatttaac aattgttcat cagtagcttc cttgatagat tgtgcatata
     5221 agctatacat ttttacttca aatttttgct tgaatttctt tttgcttatt gttaccaaaa
     5281 aaaccactcc tttaatcaca tatgtctcta tatgtttcta tatactcctt agctgatttt
     5341 ttccaactat tatctgtttt cattgcattt tcaactaatt taatccattt ttctttgtcc
     5401 ttaaatactt tgattgcatt tttcaaacag aaaaacatct catgagcatt gtaattttta
     5461 aaactaaatc cattgccttc tccagtaaat ttattataag gtgttactgt atctctaagc
     5521 cctccagtct ctctaactat tggtaaagaa ccatatctca tagctaacat ttgacctata
     5581 ccacaaggct caaataatga tggcattaag aacatatcac ttgcagcata tatctgttga
     5641 gccaatgatg cattaaaagt aattctagct gatactttat ctgaaaaatt agaatcgtaa
     5701 tagttgaaca ttgattgata ttgatgttct ccagttccta aaacgacaat ctgtaaatct
     5761 tctctcataa gttctggcat catgtatgct attaaatcaa atcctttttg agaaactaat
     5821 ctcgaaacta tacctatcaa cggaatagat gaatcttttt taagtcccaa tatatcttgt
     5881 aatctcaatt tattttctac ttttttgtca attgagtcta catcataatg aacaaatata
     5941 tctttatctt ttgcagggtc gtttaaatca taatctatac catttaatat accttttaat
     6001 tttcctgact cttttctaag taatccatct aattgttcac cataaaaaga tgtttgtatt
     6061 tcatttgcat aagtaggact aacagttata attttatctg caaaatttat accagctttc
     6121 ataaaattta ttgagccata atactctata tctctattat caaagtattt ttgtggtaag
     6181 tccaatatat catataaaac atgcttatca aatacacctt gatattggag attatgtata
     6241 gtatacatag tttttatatt tttatacccc tcaagggttg catatctttc ttttagaatt
     6301 aatggtaaca tccctgtatg ccaatcattt atatgtatta catctggata aaaatcaatt
     6361 ctacttatag cttctaaaac tgcatctgta aagaaagtat atctttctgc atcatctcca
     6421 tatccataga tactctttct ttcatcttct cttctaaaat aaaattcatt atctataaag
     6481 taaaatttta caccatccaa ttccatttct aaaagaccac agtattgatt tctccatcca
     6541 acacgtacac taaacacagc tatttctttt agttgatctt ttaaataact tggtatagtt
     6601 gaatatttgg gcattattac ccttacatct acaccttctt tttttaattc tttaggcaat
     6661 gcataagaaa catctcctaa tcctccagtt tttgcaaagg gccaacactc tgctgttaca
     6721 taaaaaacct tcattagtca ttcccccttc taaaaaattt atttatatat ttgtattctt
     6781 ttccactaca agaggatatt tttcatctcc tcttaattct ttcccatttg atacactcgt
     6841 atttttatct aatattgcat tatatagttt tgcatttgac tttattacac aattttgcat
     6901 tactattgag ttctctataa ctgttccttt ttcaacattt acctttctaa atatgatact
     6961 atcttttaca gtaccttcta ttaaacaacc agatgctata aagctatttt ctactttagc
     7021 actatctgta taaattgtag gtgattcatt cttttctttt gtaaatatct ttctttcaga
     7081 gtataataat tcatctgcaa cttctaaatt taataaatct ttgctcattt caaaatatga
     7141 ttcaacagaa tttacacatt ttagatatcc tgtatattca taagctccta ttttcatatt
     7201 ttctgcttct tcaataataa attcttctaa ataacaataa ctgcctttat ttacaatgtt
     7261 atatatacat tcaacaaaat cttcacgctt cataatatat gtctccatac atatgtttgc
     7321 tctagggaat ttgcctaaat tatttcctac atttattacc ctattgttac tatctaaatt
     7381 tagtgttaag ttaccagcaa aatctttatt tgcattattt acatttttat atattatagt
     7441 tatatcatta tgagattttt tatggtattc caatgcttga gaataattta aattgcatag
     7501 catatagctt ggagctatta aaatatattc ttcttcactc ttttctatat aatctagatt
     7561 agctaagata gtatatatat caccttctct atgtgagtaa gtatttctat acttagtgtt
     7621 ttctggacta aaaattgata ggccaccttt ttttctactt aaatcccaat ctttaccact
     7681 acctatatgg tctgtcaatg atctgtattt ttcttttgcg aatattccaa ctttagtgat
     7741 tcctgaattt atcatattag ataaagcaaa gtctattatt ctatatcttc cagctattgg
     7801 tgtagatgct attggtcttc catagttaag tttgtttatt gctgggtctc cttttttgtt
     7861 taaatttatt atccctaaac attcatttct catcttaaag acctccttct attaatacgc
     7921 atctaggttc gatattttca tactcagaaa ctacatttat ttcattattt acattcttta
     7981 tctgaacatt atcatttata gttgcacact cacctatcat agctttttct atagttacat
     8041 tttcacctat tacaacattt ggcattatta ctgaatcaat tactttactt cctttaccaa
     8101 caacaactcc atgtgataat acagagttgc ctacttcacc taaaattcta caaccatcag
     8161 ctatcattga tctatgaaca tttgcatttt tacctatata ttgtggaggc attgccatag
     8221 tatttgtata tatcttccat ttagggtctg ctaaatcaag tgtttctgga gattttatta
     8281 tatccatatt agcatcccat agactttgta tagttcctac atctctccag tatcctttaa
     8341 atggataagc atacattcca acattatctt ctaacatttt aggtattaaa ttttttccaa
     8401 aatcatcata atttatttct ggattttttt ctgcttcctt aaaataattt ctcaacattt
     8461 tccaatcaaa tatgtatact cccatagatg ccaaattgct cttaggttca cttggttttt
     8521 cttcaaattc atagatactt gaatcttcat ttgtattcat tatcccaaat cttgaagctt
     8581 catcccaagg aacttcaatc actgctattg tagctttaga gcgtttttcc ttatgaaatt
     8641 ttaacatttt gctataatcc atcttgtata tatggtcacc agataatatc aatacatatt
     8701 ctggattata ggtatctaca aagtccatat tttgatatat cgcatgtgct gtaccattat
     8761 accaatttcc ttctttttca ttcataaatg gttgtaaaac atagacacca ccatttattc
     8821 tgtccaaatc ccaatgactt cccatgccta tatgtgaatt taatatcaac ggtctatatt
     8881 gagttaaaac acctacagta tcaataccag agtttgaaca attacttaac acaaaatcta
     8941 taattctata cttacctcca aatgatacag ctggtttggc aattcttttt gtaaaaacac
     9001 ctaatcttga accttgccct cctgccaaaa tcatagctaa catctctttt ttcatttaat
     9061 gacactcccc tcatctagta attaatattt ataattgcat ttttactttt ataaacttta
     9121 tcagtcaata aatgccgtgt ataaatgttg cttaaataat accttaataa aaataaaata
     9181 atatcaacta tacaattgat ataatgtata ccccataatg aacttaccga cg
//
LOCUS       DAELSY010000150         9014 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.150, whole genome shotgun sequence.
ACCESSION   DAELSY010000150 DAELSY010000000
VERSION     DAELSY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9014
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.150"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            85..786
                     /locus_tag="KPG09_003153"
     CDS             85..786
                     /locus_tag="KPG09_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBG0967736.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            779..1102
                     /locus_tag="KPG09_003154"
     CDS             779..1102
                     /locus_tag="KPG09_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBG0967737.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(1308..3368)
                     /locus_tag="KPG09_003155"
     CDS             complement(1308..3368)
                     /locus_tag="KPG09_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBG0967738.1"
                     /translation="MEKYRALKLVSLIIISISFPLTILLATIGLAEPKYNIQKTSTKS
                     GIFNISNDFYDSNIFDEGTVESLLSNFKISVDSQLTSEEKSKLQTKSKSIDLSSNDSE
                     YDKIMINKQASEEYLSYLKNIKVYVINTYTGVFYTNTSYGNLDDFKQKTKSYCNIEII
                     NNVGKSSYIKTINGEKFELNNLSMELNGVDEAFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVACLSLAIVCLLSIILYSRIKGEVLKRNNVFLLIYSKIPMEFNVILVF
                     ISIAILTMYALAVSYHIDVIVLVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYIKVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(3564..4256)
                     /locus_tag="KPG09_003156"
     CDS             complement(3564..4256)
                     /locus_tag="KPG09_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0967739.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            complement(4868..5243)
                     /locus_tag="KPG09_003157"
                     /pseudo
     CDS             complement(4868..5243)
                     /locus_tag="KPG09_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            complement(5348..5725)
                     /locus_tag="KPG09_003158"
     CDS             complement(5348..5725)
                     /locus_tag="KPG09_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0967740.1"
                     /translation="MKEEILIEKLLRAELIVMEYLCKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINFQYHYKIIIDNKEYYTFKKKVLKSSKSIKIMRSLT
                     TTHKNVSKEKLDLLDEYYRNLKE"
     gene            6531..7025
                     /locus_tag="KPG09_003159"
     CDS             6531..7025
                     /locus_tag="KPG09_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0967741.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIIALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKNDYGNLIDITNDQNKINIVIKLLNN
                     KSNT"
     gene            7422..7572
                     /locus_tag="KPG09_003160"
                     /pseudo
     CDS             7422..7572
                     /locus_tag="KPG09_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8048..8236)
                     /locus_tag="KPG09_003161"
     CDS             complement(8048..8236)
                     /locus_tag="KPG09_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0967742.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGEGALKIKKFSILYGSS"
     gene            8398..8748
                     /locus_tag="KPG09_003162"
     CDS             8398..8748
                     /locus_tag="KPG09_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBG0967743.1"
                     /translation="MIQKKIFMGFCGFLGFLSLRYFSSGNVTDLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNVELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            8750..8950
                     /locus_tag="KPG09_003163"
     CDS             8750..8950
                     /locus_tag="KPG09_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0967744.1"
                     /translation="MPKFICNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
BASE COUNT         3112 a         1230 c          934 g         3738 t
ORIGIN      
        1 aattataatt gcaattatac aattaatcta tttttctgtt ttactaatac tttaatacat
       61 ttaatacaaa aggagtgatt tattgtggaa agaactcaac aattaaaaga aagctttatt
      121 gcttcaattc ctatattctt aggttatata cctataggta tagtaggagg aatactatta
      181 caaaaatctg gtctttcacc ttttcaaata gcactaatgg taactttagt atttggagga
      241 agttctcaat tcattgcagc ttcaatgatt tctactggtg caagtgtaac atctattgtt
      301 ttgactacat ttatagttaa cttaagacat tttctaatga gttctaattt gaacatgtac
      361 ataaaaaata aaagtccaaa atttatattg ccattttgtc atactataac agatgaaact
      421 tttgcagtta attatgaaaa atttactgct ggaaattggt ctgatagaaa tgctatttat
      481 cttaatttct tctgcttaat ttcatcagtt ttagctaatt ttattggagc atttttagga
      541 gaaagtatat ctatagacag ttctatttct ggcttcatat tgacttctat gtttatagct
      601 ttaatagttt ctcaaataaa aaataagatt tatattgtag tatttatatc atcaatcatt
      661 atttctgtta ttttatatgc attattcaaa agcaatttag taataatagt tgcatcaatt
      721 ttagcatctt taattggttt ctttatagaa gaatcttact gtaaaaagga ggaatcttat
      781 gaataataat tatatatttt ttattataat tggaatgtac attgttacat atctaccaag
      841 agtgcttcct atgttaattt tttcaaaaaa agagatgcct gattttttga aaaaaatcat
      901 gaaatttata cctgtagcaa tacttacttc cttaactgct aaagatgtat tttttaatgg
      961 agatagtcta tatttatcta tagctaaccc taaaattata tcttttatga ttgtacttct
     1021 tgttgcatat aagtttaaat ctattggtat ttctattatt acaggagtga tatctatata
     1081 tttatttgga attatcttgt gatatttgga taatgtttta ttgtataagt tattttaaaa
     1141 ctatgtgtac aattttaatt taagttgtta tattattgat tttaaataaa aaacagagtg
     1201 acttaaagtt actctgtttt tactcttcat tctctactgt aaatattcta atatattata
     1261 ataaaagctt tcgacagtaa aaaaaatata tatacttaaa tactatctca ttccatattt
     1321 gttatttctt cttcatattt ttgaaactct aaatttgctt taaataaatc tccatctatt
     1381 tctatataaa attttccacc ttgtaaagct actaaatctc ttgcaatagc aagccctaat
     1441 ccacttcctt cagtattcct tgattcatca gctcttttaa atctttccat aatctcactt
     1501 gggtcaaaat ttaattcata agaagatata tttttcatag ttacctttac attttcacca
     1561 tagtcatata catcaatata gactctagtt tcttctaagg aatattttgc tatattagat
     1621 aaaagatttt caaatactct atacattctt cttccatctg cccatatata caccttatca
     1681 tttggaacat ttaattttaa atataaatta tttttagata atttttcttc aagttcacct
     1741 atgctttgtc ttaatagttg cactaaatca atcttgctaa tttgtaattc tatattgcca
     1801 ctacttactc tactcgcttc aaataaatct tctatcaata attttagtct ttttgacttt
     1861 gactcaagta cctttatata atcatttata tactctggtt gtattgattc ttctttttta
     1921 attaaatcta cataatttat tatagaagta agtggagttt tcaaatcatg agatacattt
     1981 gttataagat cagacttcat tctttcactt tttacttgct cttctatagc tctatcaaga
     2041 ccttctccaa tattatttat attttcagct aaagacgtaa aattatcatt cccagtaatt
     2101 tttatcttat aatctaattc tccctttttt attctttctg taccttctat tacgtaacta
     2161 agatatgaaa gtcttttagt tatatatatt gtaaatccta aaaaaataca tatagaaaac
     2221 agtaatccca tacctatact tcgagtgcta attaccatga ttaaagctaa agatattatc
     2281 actataacaa taactttcct tgctaaagat actttcttta tagcgttact gacctccctt
     2341 acagttttta ttaaccataa aatcactctt actataatac ttgtcttaaa tatacacatt
     2401 ttattttcta aataactcaa ttgcacattt ataaaaaata tattatttga tactatcagt
     2461 aaagataaag aaactaatac aattacatca atatgataag atacagcaag agcatacata
     2521 gttaaaattg caattgaaat aaatactagt attacattaa attccatagg tatcttactg
     2581 tatattagta aaaatacatt atttcttttt aaaacttcac cttttattct gctatataaa
     2641 atgatagata ataaacacac aattgctaaa cttaaacaag ctacaaaaaa taatcttact
     2701 ttttctgtag cttgtttaaa tatttgaaaa ttagtgtata ctattccatc ttctatagtt
     2761 ggctcttctg gaaaagagac atatgcctca aaggcttcat ctactccatt tagttccata
     2821 ctcagattat tcagttcaaa tttttctcca tttattgttt taatatacga gctctttcct
     2881 acattattta ttatctctat attacaatag ctcttagttt tttgtttaaa atcatctaaa
     2941 ttaccatatg atgtatttgt ataaaatact cctgtatatg tattaataac atacactttt
     3001 atatttttta gataacttaa atattcttca gaagcttgtt tattaatcat gattttatca
     3061 tactcagaat cattactact taaatctata cttttacttt ttgtttggag tttacttttt
     3121 tcttcactag ttaactgact atccacagat atcttaaaat tagataataa gctttccact
     3181 gttccttcat caaatatatt actatcataa aaatcattag atatattaaa aatcccactc
     3241 tttgtagaag ttttttgtat attgtattta ggctcagcaa gtcctattgt agccaacaat
     3301 atagttaatg gaaatgagat agatattatg attaatgaaa ctaattttaa tgctctatat
     3361 ttttccaagt tgcccacctc atctacattg tcagtatttt atataaaata aagtttaaga
     3421 aaagcataat ccccatagag taaattaaac tctcagagaa aaaattaaaa catattaaaa
     3481 ttcctaacat ttgtgtaaat gtaaaaatga ctaaaatcaa aatgcaatta aatattaaac
     3541 tcttttcttt tataattttt ccactaaaat ttttcaatct tataaccaac tccccacacc
     3601 acctttaaaa atcttggttc ttttggattt atttcaatct tttctcttat tcgtctgata
     3661 tgtactgcta ctgtattttc tgaattaaaa ctttcctcat tccatacttt ttcgtatatc
     3721 tcatctattg agaaaactcg tcctttattt tctaaaagaa gttcaattat tttatactca
     3781 attggggtca tttttatgtt ttcaccatca attttaactt cttttgttag tgtatttaac
     3841 tccaaagctc cactttttaa aatttcttta catgtatctt cataattacc taaagttaca
     3901 tatcgtctta agtttgattt aaccctagct ataagctcta atagattaaa tggctttgtt
     3961 atataatcat cagctccaat atttaatcct attattttat ctgtatcttc actttttgct
     4021 gaaattaaaa ttattggaat atttctttct tctcgtattt ttacagttgc tttgattcca
     4081 tctaatttag gcatcattat atccattagt attagatgaa tgtcattctc tgttattatt
     4141 tccaaagctt tcattccatc atatgcttta tatattttaa atccttctga tttaagatat
     4201 atttctattg aatcaacaat ttctttatca tcgtctacta ctaatatatt gtacatattt
     4261 tcacttcctc tattgtataa taatacttat ctatattata caatatcttt cacttacttt
     4321 ctcaacaatg actaaattgt tatattactt tggcttacat ttttgtaact ttcccttata
     4381 actatataat aaaatataaa tcttaagaaa atattatcct aattcttaaa tatttcttaa
     4441 gatttaagat aaattgagat ttatttgaat aaattgattt gaattatgtt attttcagat
     4501 tacttttatt taagtatttt gattggattc agctgaatta atgtatatta attcttattg
     4561 aacagttttt atttttgggg tggaagaatg gtggaagaat tacatttcac taattttctt
     4621 ccaccattct tccacccaaa tatacttaga aacatttatt tcactgcaat aatagctact
     4681 tatttaaatt ctatttttat acattctatc tactatatac tgtacatatt ctattaaaat
     4741 ttatataaat aaaatacaaa cttatttcat ttttttaaca tgttcagaat aataaacaaa
     4801 atgaatgtag aaaaagttat aatttcatac cttcatttta tttattatgt atctagctgt
     4861 cattttctta ttttaaattc ttaaaatatt cttctaataa atttaaaata tcaccatcta
     4921 cttcatcatc cttactacat agtgctgaca ttaaacttaa tattgaattt ttatgtgtat
     4981 tctttaaaaa ttcttttgtt taaaatctct gatattattt ttcttttact aatacattat
     5041 agtgtgtacg cttatctatt ttctccgttt ctaaaaactc tcttctttag taatcttttt
     5101 aatactgtaa atatagttgt atctttccat tcatattttt gttccataga cttggtgatt
     5161 tctcttgata ttaatacctt ttctgattcc caaatatatc tcataacttt taattccgct
     5221 tctggtaatt tactaatttt catagtatac atccctttac caatttatgt atatgttaaa
     5281 ttctatatca ataaagtttt acaaacaaaa gaattaaggt aaataattac attcgatttt
     5341 tactatatca ttcttttaaa tttctataat actcatctaa taggtctaac ttctctttac
     5401 ttacattttt atgtgtagtg gtaagtgaac gcattatttt tatgctttta ctagatttta
     5461 gtaccttttt cttaaaagta taatattctt tattatctat tattatttta taatgatatt
     5521 gaaaatttat aatatatcta gccaaatacc ttttagcaac tagccttttt agtagtattt
     5581 ttattgtact tttatgccat ccataaatct gttttatttc ttttacaatt tcttttttag
     5641 gcattagagt gtctttttta cataaatact ccattactat taattctgct cttaataatt
     5701 tttctattaa tatttcttct ttcaatttat gtcacttcct taattcttct aacttaaatt
     5761 aataatagga gctttttaag acaaaaacta gaggtaagtt ttaccaaata ttctacatat
     5821 ttagtctgtt tttttaaacg taaaataata ttcatatata tatatttgtt taatttgcaa
     5881 acaatgctac caaacaataa atagctctat tgtattattt tagttgtaga atagcactaa
     5941 atcgttagaa tcaaagaaat ttagtagcac aaaactatat aggctaatgc ttataaaata
     6001 aattataagt tatgttaaca agttactaaa agcatatttt atttttgttt ttatgaaagt
     6061 tttttaggta tgtaataata tatatctttt aaaaatatgt attcatgtat acatattttg
     6121 acttattata ctgataattc atataattat tttgatttta tagatacaca gttagatgat
     6181 tttataatag agcataaaaa aaatgtagta taatcttgtt tatatcaagt tatggtattg
     6241 gtaagtaagt ataattaaat agacattatt ttctgggtcc tctttttatt ttcatctgct
     6301 tttaaataat attattgaca ttgatatgac tgtagttttc tttattttaa tccattgctt
     6361 atatcttttc taacaagaaa tgtcatttta aatcatttct ataaattgta aaataatagt
     6421 atatttgtca tagaaagtga tttttttgtc tatatatggt aaaatataga aagtagaaat
     6481 caacaaaata aaaatttata tattattaat tttatatgga ggggaaataa atgaaaaata
     6541 aaaaaggatt tactctagtg gaattattag tagtaattgc tataatagga atattggcaa
     6601 taatagcact tccagcatta tttaaaaata tagaaaaagc aaagatagct aaacttgaag
     6661 ctgatataag tgcaataaaa agtgcatctc ttagttacta tgctgatgaa tccaagtata
     6721 ctgatggagg aatgatatca tgggtaaaga aagatggaaa aataataata aatgggggtt
     6781 ttaaagatga cccattagca gataaaatag aaaatttagg gatgccttat aatggttcat
     6841 atctgttaat gtcatctcct ggtcatgaaa aatatctaga attaagcata cttccagaag
     6901 gagaaataag caaaagtggt ctagataaat taaaaaatga ttatggaaat ttaatagaca
     6961 taacgaacga tcaaaataaa ataaatattg taataaaact tttaaataat aaatcgaata
     7021 cttaaataca ataaatttga tttaataatt actgatgaaa taaacaatct tgtttggaga
     7081 aatgaaaaaa gcactcccca agaatagaat gcttagttat aactacagaa gtgtattggc
     7141 atacacttct attttttatt atatcacata atttatgttg ctaaaaatac ctatttaagt
     7201 tgtttttaaa ttgtgttgag taatattctt gatagtttat acattaatga atttcaaaaa
     7261 aataagcact ctttataata aaaagtactt tagtgtatgt taatgtccta tctaataaaa
     7321 ttgtaacatg tcttatgctt tagtatgata attttcattc tctttattgt tgcaacttat
     7381 actatagttt tgctaattta tctgcaaaca caacttcttt attgcatgtg tatcaaccaa
     7441 aaacatcaga atacttactt aatacatctg caaaatcgta tctttcatct atagtattct
     7501 tatttgacaa tataacaaaa aatgaatcga gatatgcctt ttttataatt agatatattt
     7561 tataaattct agcatatcat ttgttatttt ctgatatgct attgatgttt taaatcttct
     7621 atttatttta ttatttttta cttttccaat tgtaccttta taggagttat atttatgtga
     7681 tttatgaaaa aatgctatgc atttaagttc taattttttg ataatacatc taactttctt
     7741 ttgattaata atgataccac tatttttaat gtggcttccc tgaggcacac atctttttta
     7801 aaaactcaag ctcatctttt aaaaatttat tttcatattc taattcttgt tctcttgtta
     7861 atttattttt tctttagtgt ttttgttttt attcattatg gtcttccttt cattttgaaa
     7921 aaggcatcaa caccactatg taaaaatttt tgttttcatg ttgctataac tgtgctattg
     7981 tttattccaa aatacttagc gatttattgt acactatact tagttatcat catatagttt
     8041 ataacatcta acttgaacca tatagtatag aaaacttttt tatttttagt gcaccctctc
     8101 ctaatatatt aacccatctt tcaacttgag aatggtcata aactccatat tgttttacta
     8161 ttaaaatatc tcttccttct ctattcaaat aaaatttagc tacttttaat ttgaaattaa
     8221 aatcatattt agtcatacat aaacatctca agcgttagat ttttactcta acaattatgg
     8281 tgcagtacaa atattgtata atactgattt tctattgaca aaagaatata aaagagatat
     8341 atttgtatta taaagataca aatatatctc ttttaaaatt actaaggagg ataaaaaatg
     8401 attcaaaaaa aaatttttat gggtttttgt ggtttccttg gatttttaag tcttagatat
     8461 ttttcatctg gcaatgttac tgatcttaca tatattggat tttttgcttt tttctcaaac
     8521 tttattatag caaaaataaa tggtgataaa gcagatgaaa ggtatgtaca agatgaaaaa
     8581 gcagctatgg catttacagg acagcttgct attatagaat tattcatttt atggtgtatt
     8641 actatagtgt ctaggaatgt agaattaatg tgtgttttac tatcaattac atatgcaatc
     8701 actttaaatg tttatgctat aaaactttat attttagagg aaaaataata tgcccaaatt
     8761 catttgtaac ctaaaaaaat atagacaatt aaaagaatta actcaagaac aattagcaga
     8821 aaaagtagga gttagaagag aaaccattat gcgtttagaa gcaggaaagt ataatccttc
     8881 tttgaaatta gcaatagata tatcaaaaat agtagataca cctattgatg aattgtttat
     8941 ttttgattag tttttgcatt attaaaaatg tgttttaaat acaaacttaa attcaatctt
     9001 taagtcactc ctga
//
LOCUS       DAELSY010000151         9009 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.151, whole genome shotgun sequence.
ACCESSION   DAELSY010000151 DAELSY010000000
VERSION     DAELSY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9009
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.151"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            32..1660
                     /gene="aspD"
                     /locus_tag="KPG09_003164"
     CDS             32..1660
                     /gene="aspD"
                     /locus_tag="KPG09_003164"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBG0967745.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDEI
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1781..2776
                     /locus_tag="KPG09_003165"
     CDS             1781..2776
                     /locus_tag="KPG09_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBG0967746.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGIGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(2934..3602)
                     /locus_tag="KPG09_003166"
     CDS             complement(2934..3602)
                     /locus_tag="KPG09_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG0967747.1"
                     /translation="METVSNNQSSVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(3701..4013)
                     /locus_tag="KPG09_003167"
                     /pseudo
     CDS             complement(3701..4013)
                     /locus_tag="KPG09_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431045.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4440..6860)
                     /locus_tag="KPG09_003168"
     CDS             complement(4440..6860)
                     /locus_tag="KPG09_003168"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBG0967748.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(7214..7564)
                     /gene="rsfS"
                     /locus_tag="KPG09_003169"
     CDS             complement(7214..7564)
                     /gene="rsfS"
                     /locus_tag="KPG09_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBG0967749.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(7681..8253)
                     /gene="yqeK"
                     /locus_tag="KPG09_003170"
     CDS             complement(7681..8253)
                     /gene="yqeK"
                     /locus_tag="KPG09_003170"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBG0967750.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSRNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(8254..8943)
                     /locus_tag="KPG09_003171"
     CDS             complement(8254..8943)
                     /locus_tag="KPG09_003171"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBG0967751.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
BASE COUNT         2960 a         1532 c         1204 g         3313 t
ORIGIN      
        1 gtgaaactta ttacataagg aggaatttaa catgaaaaat catacttata aagaaatcaa
       61 aaacatatat ggaaaaataa gcccttttga atttaaggac aaattaatag atattgcaaa
      121 atacactgca aaagaaaata acagagaatt acttgatgca ggtcgtggaa atccaaactg
      181 gacttgttca actgctagag aagccttttt tacttttggt catttcgcta ttactgaaac
      241 tcgttctaat tgggatttag gtcatttggc tggaatgcct caaaaaaagg gtattaaaga
      301 aagatttttt aaatttataa atgaaaatat agatatgcct ggagcatatt tagctagaga
      361 tattataaac tttggtataa atgaacttgg atttgatgga gatgaatttg ttcatgaact
      421 agctgatgga attattggtg ataactatcc acttccagat agaatgttac ctcatatgga
      481 aaaaatagta cacgattacc tagttcaaga aatgaaatat gatatatcag gtaaatatgg
      541 tgatgtagaa atttttgctg ttgaaggtgc tacagctgct atgtgctata tatttgattc
      601 attaatggca aatgaacttc taaaaaaagg agatacaata gccttaatga ctccaatatt
      661 tacaccttat cttgaaatac ctaaccttcc tcgttacgac tttaaagtgg taaatataaa
      721 tgctaatgaa gttgatgaaa aaggtgctca tacatggcaa tatacaaaaa aagaattaga
      781 gaaacttcgt gacaaatcta ttaaagcttt attcgttgtt aatcctaata atcctgcttc
      841 tatagctatg gatgaaatat cttgtaataa tctaatagat gtaattgaaa attataataa
      901 agatttaatg ataatatcag atgatgtata tggaactttt gtagaagatt tttcatcctt
      961 aatgtcaaaa ctaccttaca acactgttgg agtatattct tattcaaaat attttggagt
     1021 tacaggatgg agacttggta catttgccct tcataagaaa aatgtttttg ataagaaaat
     1081 aaatgattta actggagaat taaaaaaatc agtagataag cgttacagtg atatgagtct
     1141 taacccttct agtctttcat ttatggaaag agttgttgct gatagcagac ttgttgctct
     1201 caaccatact gctggtcttt ctactccaca gcaagtacaa atggcgtttt tctctgcttt
     1261 tgcattgatt gacaaagtag atgcctacaa aaaactcaat atgagtatct gccatagacg
     1321 tcaaaaacta ttatttgaag ctttagagct tccaattaat gaaaacaaaa acaatgctgc
     1381 atattacact caatttgata ttgaagaatg ggctaaatta aactacggag aagacttatt
     1441 taaatttata agtaaaactg catctccagt agatgttctt tataaattag ctaatgacta
     1501 ttcagtagtt ttacttagtg gaaatggatt ctatggccct gaatggtcaa ttagaatatc
     1561 tctagcaaat ctatatgatg aagcttatac taagatagga aaagccataa gagagatatt
     1621 aaatggttat atcgttgaat ggcaaaaaat taagaaataa ttatattaca ctgaaatatc
     1681 taaattatat gaataaaaga gtttatgtag accttaacta ctttttttga tataatccta
     1741 tcggaattat aaattttatt tatgaaaggc agagaatcgt atgagtatta agaaaaaagt
     1801 tgccatagtt tttactggtg gaactatatc tatgacagta gatgataaag tcggagcagc
     1861 tataccaact ttatcaggtg aacaaatact atcaatggtt accaacattg ataaagttgc
     1921 agatgttgaa gtttttaatt ttgatgaaat tccaggacct cacataacac cttatagaat
     1981 gatggaattg aaaaattatg taaatgattt attagcaaga aaagatatta caggtgttgt
     2041 aataacacat ggtacagata gtttagagga aacagcttat tttctagacc ttacaattga
     2101 caacatcaaa cctgtaatag tcacaggtgc tatgagaagt agttctgaac ttggatatga
     2161 tggttcaagt aatttatctg catcagtttg tactgcagta tccaaagatg ctatgggaaa
     2221 aggagtcctt gtagttttaa acaatgaagt attattagct tctgaagcta ctaaaacaaa
     2281 tactctttct ctaaatacat ttaaatctct aactagtggt ccattaggaa taattgactg
     2341 caacgaactt gtccttacaa gagatattgt aaatagaaca attatagata ctgataaggt
     2401 agaatctaaa gttgccttat ttaaagcata tgtaggagaa aatgctgact ttataagatt
     2461 tgctgttgat agtggttata aaggtatagt tattgaggca atgggtatag gtaatattcc
     2521 tcctcaaatg ctttctggag ttgaatatgc tagagaaaaa ggacttcctg tagttatagt
     2581 ttctagatgt cattcaggta gagtattcga tagttatggt tactttggtt ctggtagaga
     2641 tttaaaaaat cttggttgta tatttggtgg ggacttgcca ggtcaaaaag ctagaataaa
     2701 acttatctta gcacttggaa aaactgataa ccttgatgaa atcaaagatt tctttgaaaa
     2761 aggaatctat tgctaattct aataacttct atttaattat ttagttcagt ttacaagaaa
     2821 atacacacaa accataaaat gagatgttga caacataaca gacagtatgc tcctgtaaaa
     2881 ggtaacatac tatctgttat attgtctgga ttcccaaatt agctatttgg aacttaacta
     2941 aaaaatccta atataagggc aactattata atggcaatcg gaatacatac tgtgtaatcc
     3001 tgttccatag agtcatcatc tgaatgtagt tttacaattc gcaatttagc aatcagtttg
     3061 aagcagaaat agcctattat tgttccaatt acattcgtga gtaagtcgtc tatatcggtt
     3121 gctctgtgta acgtaaaaag ctgaatagtt tcgataaaga gagataatcc aaaaccaatc
     3181 aagaatgtat ttctcaaccg ttcaaaggtt ttgctgataa gcggacataa aaaacctaat
     3241 ggaacaaaaa gtaaaatatt caaaataaaa ctcaaactaa atccatcact aaaaggaata
     3301 agatttatgt ttggattaaa gaacgtttcc cctaactgtg acagtcttat atattcactg
     3361 aatgtaggga taccaacaat atgcgatagt gttaaacata agtagtaata cattgccagt
     3421 gataaaatag atgtttttac ctttggaata tcactatttt tcttgaatat tacaactaag
     3481 aaaataccaa tcaagataat tcccaacaag aaaataggat taattaacaa taaatctttg
     3541 atagcttcaa acagactgag ttgaatttct acacttgatt gattatttga tacagtctcc
     3601 atttgtacac accccctcat aacatttcaa ttatttctaa aaaacaaaat agaagataaa
     3661 tcttaaaatc atcctatcaa aatttttaaa tttgccttta tcagtttata ccaccagtta
     3721 attcctgtct tacttctgta tggtattctt gggattcttt catcaaatta gacaaaaaaa
     3781 tggttaccat atcaataaac tggttacgct ccatatctcc tgaagcaaac atcccctgag
     3841 aataaatccg tatcaggaat ctccacatct actgcaccta tcacaagtca tttgataaca
     3901 tcctcaaaca atcccaactt ggatatatca gcaacaccca ttagaatact actgctgacc
     3961 atccttgttt tcctggtgat aattttaatc aactgggaag ttgaaacgcc tatctttttt
     4021 gcaagttcct ttttagaaac atggtagtta ttatatgaat ctgaatctcg ttgtctgggt
     4081 gtttaagata aagcatagat acgcaccttt tttgcctgtt ttcattatat aaaaaaaatt
     4141 ttaaaaatac aataattata tttttttcgt ttaagcttag agcagttcct tgatgaacaa
     4201 cactttagaa actgaataat ttacatacca cttaatacat taacacgatt taacattaat
     4261 tataataata tttaaaacaa caattagagt gtataagaat gaacttttta gttctttaat
     4321 catttttttg tataaaatca aatctatttt tgtaatttca acaatgaaaa aaggcttatc
     4381 tctattttga gacagcctca tttattttat aaatttatat ttttgttcat atttttgagt
     4441 tatttaacaa ctatatttac taatttttta ggtacagcta caactttaac tatagtttta
     4501 ccttcaacta atccttttat tttttcatct tctaaagcta ccttttccat ctcatcttta
     4561 gatatatttg agttaacagt taattttcct ctaacttttc cattaacttg tacaacaact
     4621 tctatttcat cttttactaa agctttttcg tcatatttag gccagtctat atcaaataca
     4681 cttccttctt tacctatcat agtccatagt tcttcaccta aatgaggggc aaatggtgca
     4741 attatagtta ctatagtttg tacaccttct tttataactg cttcgtttat attatctaac
     4801 tctttatatt tatacatatc atttattaat tccataagag ctgatatagc agtattaaat
     4861 ccgaattttt cacttaaatc agcagtaact tttttaagtg tagagtgtat agtatatctc
     4921 atatccttat cttgggaatt taattctcca aactctacat cctttttaac cacatctgct
     4981 aactcgtcaa ccaatctata aaccctattt aagaatctaa agcatccatc aacaccttgc
     5041 tctgaccagt ctaaatctct ttctggtggt gctgcaaata atacgaaaag tcttgcagta
     5101 tctgctccat actcatctat tatatccata ggagatactg tattcccttt agacttactc
     5161 attttagaac catccataag taccattcct tgagtaagta agtttttaaa tggctcattg
     5221 aagtctacca ttcccatact cttaaatgcc tttacaaacc atcttgcata aagcaaatgc
     5281 attatagcat gttctactcc acctatatat tggtctactg gatgccatct attaactaat
     5341 tccttactaa atggttcatt ttcattattg ctatccacat atcttaagaa ataccaagaa
     5401 gaatcaacga atgtatccat agtatcaact tcccttctag ctggttttcc acagtgagga
     5461 caagttgttg acataaattg ctttgaagtc gtaagtggag attctccctt tccagtaaat
     5521 tcaacatctg ttggtaaaag aactggtaaa tcttcctttt taactggaac tattccacat
     5581 tcatcacaat aaaccacagg tataggacaa ccccagtatc tttgtcttga aactaaccag
     5641 tctctaagtc tataattaat tgttctcttt cctctacctt ctttttctaa cttatcgatt
     5701 attccttcaa atccttctga agcatctaat ccattaaatt cttctgaatt tatcattttg
     5761 ttatcttctg ttattacatc aattatgtct aaattatatt tttcagcaaa atctctatct
     5821 ctttcatcat gtgctggaac tgccattata gcacccgttc catactcaac taatacatag
     5881 ttagctatcc atagaggaac tttcttgcca tttaaaggat ttataacata tctacctata
     5941 aacattcctt ctttttctac atcagctgct gttctttcta tttctgtcat agtatgcatt
     6001 ttttgaacaa aagcttcaac atctgcttca tattcagtcc ctgcaaccaa ttctttaact
     6061 aattcatgct ctggagcaag taccatataa gtaactccat aagttgtgtc tggtctagtt
     6121 gtaaatactg tcatagattt atcagttcca tctatatcaa aaactaaatc tgctcctgta
     6181 cttttaccta tccaattttt ttgcattgtt ttaacttttt ctggccatcc atctaatgta
     6241 tctaagtcat taagtaattc ttctgcaaat tcagtagtct taaaatacca ttgttctaaa
     6301 tctttcttta atactgttgc tttacatctc tcacatgctc cttgaacaac ttgctcattt
     6361 gcaagaacag tctcacaaga aggacaccag ttaacatatg attttttctt atatgcaagc
     6421 ccatgctcta aaaacttcaa aaatatttct tgtgtaaatc tatagtattc tggagttgaa
     6481 gttgcaactt ctttatccca atcataacta atacctaata aatttaattg ctctttcatt
     6541 tcttctatat tttccattgt ccatttatgc ggatgtattc catgttttat agctgcattt
     6601 tcagctggta atccaaaaga atcccatccc attggatgaa gtacattata tccttccatt
     6661 tttttgaatc ttgcgacaac atcacctata gaataatttc taacatgtcc catgtgtatt
     6721 tttccagatg gataagggaa catttctaat gtgtaataat taggtttttc agtttgtgca
     6781 gtatccattt tatactgatt attatctttc caaatttttt gccatttcga ttctacttct
     6841 ttaaaattat atacgctcat attttgcctc ctatttttaa ttgtgtataa ctaccttatt
     6901 tagtattatt tacttataat ttccaaataa ttctttgaaa ataaaaaatc ctcgtcacta
     6961 aacttacgtt tagggacgag gataaatctt cgcggtacca ccctaattag cctactacat
     7021 atatagactc tcttagatag cttttaatcc agccatggag aatagatttt aatctagtaa
     7081 gttcaaagtt atataatatt agttctcacc ttccactaac tctctgaaaa aatataatct
     7141 tttactgctc tagtctattc aatttaatat aatttttgaa attttctaat tataataaca
     7201 tatattatca tgtttatgct aatgtgtcaa tatctatata aggtgcatct ttccataatt
     7261 tttctaaatt ataaaaccct ctattttcct cattaaatac atgaaccatt atgtctccat
     7321 aatcaaggag tacccaagtt tgagttcctt gtccttcttt acctcttggc tctaatccta
     7381 gttttgctaa ttcatcttcc acattgtcag caatagcttt tacctgtctc tgagaagatg
     7441 ctgtagtaat aataaaataa tcacacaaac tagaaacttt tcctatattt attataactg
     7501 tatcttgacc cagtttatct tctatggcat catatgctat ttttgtcatt tgttccactg
     7561 tcatataaaa attcctcact ttcctaatta aaatacactt agttattgta acatattaat
     7621 acgattttgt aacatagtca aatttaaatt tattaatatg tttgccttta tatagctttt
     7681 ttataataac ttttctttaa ttagatagtt tcttgccata actgttcttg gatgtatctc
     7741 ttctttttta ttgatgacaa acattaaagt attattaaaa gaagttaaaa gtgcttcatc
     7801 aagcttccct ccatatgcta attctcttaa tgtatctaca actggaaatt ttcttccttc
     7861 ttcaattaaa tcagctatgt aaattatttt ttctaaaaga gacatgtcct ctctccctgt
     7921 tgtatgatat ttgattgcat ttataatatc ttcatcttct acttcaaatt catactcgca
     7981 tatgtacgcc ccaattacac tatgagacaa ggaacgattt ccatcctcta ttgggtctaa
     8041 atatatttca tacttattta cataatcctc tatttccttg tcacttaaat actttgcaca
     8101 atcatgaatc attcctgcta agtaagcttt ttctttgtca catccataaa tctcacataa
     8161 tttttcagca cattttgcaa catttcttga atggctaagc ctccccactg gtagcatttg
     8221 atttagtctt tggttaatat tttctaagtt catttaatca tctccatatt gatatagttt
     8281 attttcatat aaataatttt ctacattttc gggtactaag taacgtattg acttgccttc
     8341 atttagctgt tcccttatat atgtggacga tatatccagt gatggaacat aaactgttat
     8401 aatgtcagca ttatacttct tagttagttt ttctatagtc tcttgtgacc ttaacaaact
     8461 aattcctggt ctagttgcgg ctataaaagt agctaactca aagtttttct ttacatcttt
     8521 ccactcttca acactacaaa ttgcatctgc acctgttata aaataaatgt ctgcattttt
     8581 gtattttttc tttaagtatt ttaatgtatc tattgtataa gtccttttct ttctttcgat
     8641 ttctactttt gaaactaaaa aatcttcgtt gctttcagtt gctagaagtg tcatattata
     8701 tctatcatac ttatctgtag tgatacataa tttatgtggt ggatttcctg atggtataaa
     8761 gattattttg tcaatgtcat acttgtctct aataaattct gctgttgcta aatgagcata
     8821 gtgtataggg tcaaaagtac ctcccaatat acctattttg attttgcctt tatggcgatt
     8881 aaatttctct agtttttcag tatttttcaa ctctgccatc ttaactagat tttcacttct
     8941 catattttgt ctccttttaa tgttataata ctattgtacc attatttttt atttcttaaa
     9001 gcttataat
//
LOCUS       DAELSY010000152         8931 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.152, whole genome shotgun sequence.
ACCESSION   DAELSY010000152 DAELSY010000000
VERSION     DAELSY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8931
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.152"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            180..1241
                     /locus_tag="KPG09_003172"
     CDS             180..1241
                     /locus_tag="KPG09_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBG0967752.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGRKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNRLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            1332..2945
                     /locus_tag="KPG09_003173"
     CDS             1332..2945
                     /locus_tag="KPG09_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0967753.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            3220..5205
                     /locus_tag="KPG09_003174"
     CDS             3220..5205
                     /locus_tag="KPG09_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG0967754.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLSFGILEKPVVWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMIEELAKVKSFDEFYKIITKRR"
     gene            5210..5644
                     /locus_tag="KPG09_003175"
     CDS             5210..5644
                     /locus_tag="KPG09_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBG0967755.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            5673..6233
                     /gene="srlA"
                     /locus_tag="KPG09_003176"
     CDS             5673..6233
                     /gene="srlA"
                     /locus_tag="KPG09_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG0967756.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            6297..6665
                     /locus_tag="KPG09_003177"
     CDS             6297..6665
                     /locus_tag="KPG09_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HBG0967757.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            6690..7256
                     /locus_tag="KPG09_003178"
     CDS             6690..7256
                     /locus_tag="KPG09_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG0967758.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            7297..7662
                     /locus_tag="KPG09_003179"
     CDS             7297..7662
                     /locus_tag="KPG09_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG0967759.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTNDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            7763..8536
                     /gene="srlD"
                     /locus_tag="KPG09_003180"
     CDS             7763..8536
                     /gene="srlD"
                     /locus_tag="KPG09_003180"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBG0967760.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIETEFGCKSVGYGCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
BASE COUNT         3566 a          917 c         1649 g         2799 t
ORIGIN      
        1 catattattc gtatcctgta tacataaaaa ataaaaaatt gtcgctttaa agtataaaac
       61 agattatatg ttaataatgt ggtgaaatta tacccatata taaataaaat aaaacaaata
      121 aatagctaat taaggagaaa atatttttta ttttttgttt aggataaata aattttatta
      181 tggaaaaaag aaaaagcata catcttcaca aattgaatat ttatttaata gggggaggtg
      241 atagattgtt tatcacagtg gtattatttt tagtaggttt cctattaatt actaaaggcg
      301 cagatatctt tataaattgc actgtagaga taggtagaaa aacgaatata tcagaaatta
      361 tacttggagc tactatagtt agttttgcaa caacattacc tgaatttaca gtgtctctac
      421 ttgcttctat tgatggtcat actactatga gtttaggaaa tgcagttggt tctataatat
      481 gtaatacagg attagcatta gggttggttg tttttataag tccatttaat gtagataaaa
      541 aaatgttttt ttctaagtct ttactattaa tagtatccgt tatagtttta attttattga
      601 gtttagatgg agttataaca agaggtgatt cattattact tataataata ttaatctttt
      661 atatggttaa taattataga agtgtcgttg gaaaatctga tactaaaaat agaaatatta
      721 aaaataacac tattaaagac atatctacta aaaaagggaa aaattataga ggtttttccg
      781 tattagaaat tgtaaaaata ctattacttt ttgctacagg attgataatg atgataatag
      841 gttcacaaat ccttatagag agtggagtaa taatagctag ttttttaaat atacctcagg
      901 gtatagtaag tttaactata atagcacttg gaacttctct gccagagata gtttcgtcga
      961 ttactgctat aagaaaaaat catcatgaaa tatcagtagg aaatattttg ggtgcaaaca
     1021 tattgaatat agtatcagta atagctgtat cagcaatacc aaataacata ccaatactat
     1081 ctcaaaatag acaattggat ataccattca tgatattatt attattaata gtaataatac
     1141 caacacttaa atctaataga cttagtagaa tacaaggtat attgatgtta ttcacatatt
     1201 ttctgtacat atcaattttg tatttcatgt atattattta actatactgt ttaaaatatt
     1261 aattgcagaa tatcctaaaa aagtatataa tggataggtt aagattaatt gataacggag
     1321 aggaataata tatgaatata acaaagtttg aagatttacc aataagtgag ggaataaaaa
     1381 aagctatagc tgaaatgggg tttgaagaac catctcctat acaagcacag tctatacctg
     1441 cgattttgtc aggtaaagac gttataggac aagctcaaac tggtacggga aaaacagctg
     1501 cttttagtat ccctatacta gaaacaatag acccaaataa tagaagttta caagctgtag
     1561 tgctttgtcc aacaagagaa ctggcaatac aagtatcaac agaaataaga aagttagcta
     1621 agtattcgca cggaataaag acattaccta tatatggtgg ccagccaata gatagacaaa
     1681 taaaatctct aaagagtggt gtgcaagttg taataggtac accaggacgt acaatagacc
     1741 atatcaatcg taaaacatta aagatggata atgtaaaaat gattatatta gatgaagcag
     1801 atgaaatgct agatatggga tttagagaag atatagaaat gattttaagt aagattccag
     1861 aagaaagaca aacaactttc ttttcagcta ctatgccaag aggaattttg gaattaacta
     1921 agagatatca aaaagaccca gagcatataa aagttgttag aaaagaactt acagtttcaa
     1981 atacaaagca atactatata gaaactaggt catcaaataa attagaagta ttatgtagat
     2041 tagtagatgt atatgaccct aaactatctg ttgttttctg taatactaag agaaaagcag
     2101 atgaattggt aggagattta caagcaagag gatattttgc agatgcatta catggtgact
     2161 taaaacagac tcaaagagat atagtaatgg ataagtttag aaatggtaca atagatatac
     2221 ttgttgctac tgatgttgca gctagaggta tagatgttga tgatgttgaa tgtgtattca
     2281 actatgactt accacaagat gaagagtatt acgttcatag aattggtaga actggtcgtg
     2341 ctggtagaga aggtatgtca tttacttttg tatttggaaa agaaatgaga aagatgaaag
     2401 atatagaaag atatactaaa tctaaactaa taaagcataa tataccaaca ataactgatg
     2461 ttgaagagaa aaaagtaggt actttctttg cacaagttaa acaaactata gaagaaggtc
     2521 acttaactaa acaattacaa tggttagaag gtttctgtaa tgatgaagat tatgcaatgg
     2581 ttgatatagc tgctgcactt gtaaaactat ctctaggtga agaaatgaaa gaagaaataa
     2641 tagaagaaaa acctagaaga gagcgtggag atagaaaagg tggtactgga gctaaagatg
     2701 gtatgattag attatttatc aatattggta gaaatcaaag agtacaagca aaagatatag
     2761 ttggtgctat agctggagaa gttggaatac caggtaaagt agtaggtact atagatatat
     2821 atgataagta tacttttgtt gaaataccta aaaaagatgc aaaaacagta attgaaaaga
     2881 tgaaagatat taagataaaa ggtaataaga ttaatataga aaaagcaaat aagaagaaaa
     2941 aataacgtat agtatacaaa actataatta gaaaatatat aaaataatat attggatatt
     3001 taaaaagcct gatgtgtttt caaaacagat agtagataat ctgtaagagt taacagcata
     3061 aggcttttct tgttttatat gatattatga ttttccagat cgttgtgaaa aatgtatatt
     3121 agttcctttt tttcaaatct gatttgaaaa agagaatatt aattatctat atagtttaga
     3181 ttataataaa aaatgtaaga taacaaaggg gagaaaaaga tgtcaatgac ttatatggac
     3241 cctagggttt ttttgatttt agaaatggtg ttagaagaag acacaaatac tctagaacaa
     3301 ttttcaaatg ttttgggtgt tagtactagg actatcagaa actatataaa ccagattaat
     3361 cgagaattaa aaggtggtat tgctaaaata gttaaaaatg aaaaaggtac atatagtata
     3421 agaattgagg atgaaagtaa attaattgag attgtaaatt ttaatagagg aaagctatca
     3481 aattttataa atttaaattc acctgatgaa agaataaatt atgttcttga tattttaact
     3541 atgaccaaca atccaattac catagatgat ttggctgaag agatatgtat aggaagaaca
     3601 acattaataa aggatttaaa aaaagcagac agaattttag ctgaatatga tttagaatta
     3661 aaaaggaagc caaatatagg tatgatttta gttggtgaag aaatggatat aagattatta
     3721 atccttgata gattgtatga aaattatatt gatgtcttgg aaaacgttaa tcagataaat
     3781 ttagttaaag attatgatat tgaatgtctt agagatgaat taaaaaagtt gtttaaaaaa
     3841 gaagaactct acataacaga acaggtcttg agagatgttg aaagatatat aatcatgtct
     3901 gtcttaagaa atctaaatgg gtacaaattt gaaaagatag ataagagatt tgaagtgata
     3961 agatgttcag atgaatatac tttaggatta aagctcaaag ctttattgga aaagatattt
     4021 aatatagttt taaatgaaaa agaaaccata tttttgacaa tgccattaat aggtagaaaa
     4081 gctcctagta caaaattagc acttagcagt ataaagataa atgatagcgt aaaggaagtt
     4141 gtagatgaag taactagttt tttattagag acctcaggga ttgattttaa agaagataaa
     4201 aagcttatag aaaatttgga atatcacttg tattttgcac ttaatagaat gagattcaac
     4261 ataagggtaa aaaatcctct tttacaagaa ataaaggaaa gatatacatt tccatatagt
     4321 gttgcaaaga tagctgcaat gataattgag gaaaagtttg acttgaaaat atgtgaccac
     4381 gaaataggat atatagcatt acattttgga agttattttg aaaaaaattc aagaaaagtt
     4441 ctaaatgtaa atagggtagc tgttgtttgt ggaacaggtc ttgggactgc acaacttatt
     4501 agaataaaga taggaaaaat acttggagaa agtgtagaag taaatacttt ttctgatata
     4561 gaagctaaag ctaatttaga tttattaaag tattatgatt tagtatttac aacagtagat
     4621 ttcaaaacag acttagatgt tccaattttt agagtaaatg cgttatttaa tgaagaaagt
     4681 ataaagaaag agatagaaaa tgcaatactt ttgaaaaata gtataaatct taagtctata
     4741 aaaaagattg aaaaaaatat cccttttata ggtaaattaa ttgaagagga taagttcttt
     4801 atattgaata agagtacgtt tatggaaaat ttagaagata tgttagaaaa attagttcat
     4861 aaaggaatta ttgatataga gttcaaaaaa aaggtattaa aaagagaaaa gaaatcttca
     4921 acagcattag ataattatat agcattgcct cactcagtta atactaatgg ggaaaatctt
     4981 tatctgtcat ttggaatatt agaaaaacca gtggtttggg atacaaagga aattagaata
     5041 ataatattga tgataattcc agataagaat gtagatagta cagacctgat aataaaaagt
     5101 tatgaagaag tacttgaagt agggagagat aaaaaaatga tagaagaact tgctaaagta
     5161 aaatcatttg atgaatttta taaaataatt accaagagga ggtaataata tggacccagt
     5221 aatttttatt atagcatcac ttattacagt gtatttatta aatatttttc ttggatactt
     5281 acagcttaaa gattttaata aaaattatat tgaactaaaa agaaaaggaa gagtggcaat
     5341 aggcagaaaa aaaggaagaa tttcatcagg tacaattgtg ttgatactta taaatgatga
     5401 tggtgtgatt gtcgaaacta gaaaaatgca aggtgttaca gtattggcaa gagtaaagtc
     5461 atttgaaggt ttggtaggaa agagcttaga gtacatagaa aaatcagatt taactgttta
     5521 caataaacta cttaaaaaag caatactaga tgcagtcaaa cagtacataa catttgagaa
     5581 aaataaatgt gaagatacaa ataatctgag tactgatgaa gagtgtagga tggaagtagt
     5641 ttaaaaaaat atatttataa ggaggagtta atatgacgca attttttcaa gtattagcaa
     5701 gtggtgcaga tagttttatg aatttattta gggcaggtgg gcaacaattt gtaggttttg
     5761 taacagatat agtaccactt ttagtagcat tattagttac aatgaatgca attatcaact
     5821 ttattggtac agatagagtc gagagacttg caaaaaaatg ttctgcaaat atatttacta
     5881 gatatttgat attaccagtc cttggtacat ttgtatttgc aaacccaatg acactatcac
     5941 taggaagatt cttgccagaa aagtttaaac ctagttactt tgcagcagca agcttttcat
     6001 gccatacaat gaatggatta ttcccacata tcaatccagg tgaactattt atatatctag
     6061 gaattgcaaa tggtataaca actttgggat tctcaacagc agatttagca gttagatatt
     6121 tgttagtggg tatagttgca aactttatta aaggtgtagt tactgactat acaacaaaat
     6181 ttgtagagaa acaacaagga atagcattaa attcagatat aaagattgga taattacttt
     6241 aaggtataaa taaatttttc aaataaaaag atagtgtata gaggaggata aattatatgg
     6301 catttaacaa agttaaaata gtaaaaggtt ctggaggatg gggtggacca ttgataatag
     6361 aaccaacaga aaagaagaat aaggtagtat atgtgactgg gggagcaaag ccagaaactg
     6421 cagtaaaaat agctgaactt acaggatgtg agttaataga tggattttca cagggtgtta
     6481 aagatgatga aatcgcatgt gtaataataa attgtggtgg aacattgaga tgtggtatat
     6541 atcctcaaaa gaaaataccg actataaata taatgaaaac aggaaaaagt ggaccattag
     6601 caatgtttat aaaagaagat atatatgttt caggggtaaa accaacaaat gtagagcagt
     6661 tataattgag gtagtaggag ggaagaaata tggcttatga tactaaaaag aagataagtg
     6721 agcagagtca aggaattgtt gcaaaaatag gtatagcact aggaaatgtg acaagtactc
     6781 tttttcaagc tggtagggat actatagata cattaataca tactatactg ccttttatgg
     6841 ctttcgttgc agtcctaata ggtataataa atcaatcagg tataggaaat tgggtagcca
     6901 atttacttac tccattaatc ggaaatgttg gaggtttagt tgtcttatca ataatatgtt
     6961 catttccgtt gttgtctccg tttcttggac caggagctgt tatagctcag atagtggggg
     7021 tattggttgg tacagaaata ggaaaaggaa atattccacc aagtttagca ttaccagcat
     7081 tgtttgcaat aaactcacaa gcagcttgtg actttatacc agtaggtctt ggtcttgcag
     7141 aagctgaacc tgatacagtt caagtaggag ttccgtcagt tttatattct agatttttaa
     7201 caggtgcacc aactgttcta ttagcatggt tagcaagctt tgggttatat aaatagaaga
     7261 atactttata aataattaga gagaggttca gttgatatgg aattaatata cagtactcaa
     7321 ataaataaaa taggagataa tgcaaatgaa tttttacaac ataatatgtt tgtaactttc
     7381 aaagataatg ctcctcaaga gttagaaaat tactgttata tacattctga aaataattta
     7441 gtaaaagaaa tacttacgaa tgatgtgtta tcaataaata atgttgatta caaaattact
     7501 tctgttggtg agttagtaaa ccaaaatctt tcagaattag gtcatataac atttaagttt
     7561 acaggtgaaa aagatggagc aattggaggg accttatatc ttgaagaaaa agaaatagca
     7621 cctattggcg taggtactat actaaaaata attagaaaat aaaatttaaa acgaatctaa
     7681 tcatattata agtaaattat agataaaagt caatttaact aatcaagtta acttttaatg
     7741 tgataaaaaa ggggaaatga aaatgagaaa agtggcttta gttgttggag gaggaagaag
     7801 tttaggtgaa tatttatcta aacatttagc taaagtaggt tatgatgttg ctattgcaga
     7861 tttaaatgaa gacaacgcaa aaaaagtagc tgaagaaata gaaactgaat ttggttgtaa
     7921 atctgtagga tatggttgta atgccataga tgaaagacag gttataagca ctgttaaagc
     7981 tattgaaaat gattttgata gaatagattt attagtgtac aatgcaggag tggctgtaag
     8041 taaaaaaata actgattttg gatacaaaga ttttaaattt attgtagacg taaatttaaa
     8101 tgggtacttt ctatttgcaa gagaagtatc taaagtcatg atagcaaaag gtattgaggg
     8161 tagaatcata cagataaatt caaaatcagg tagagtggga agcaagcata attcaggata
     8221 ttcagcaggt aaatttgctg gagtaggact tactcaaagt ttagcgctag atttggcaga
     8281 acataagata actgtaaatg cactgatgct cggtaattta cttgattctg atatgtttga
     8341 aagtttaatt cctcaatatg cagtaaaatt gggaatacct gaatcagaag tcaagcaagt
     8401 ttatatagat aaagtacctt taaaaagagg gtgtagattt atagatgttg caaatatgct
     8461 tacattttat gcttcaaagg aagctgaata ctgtactggt caatctataa atattactgg
     8521 tggacaagtt atgtaaggtt tttatggagt gtctcaagat gaactcttta gttcatgagg
     8581 cacttttttt gtataaaatt aaatatattt tcatcattta aacaatgaaa aagaggttta
     8641 tctttatttt gagatagcct catttttgta attaagaaac atagtgttta agtaaacata
     8701 taagattgtt agttttttaa aagagaattt tttaataata ttatatttat gaagtatttt
     8761 taaacttaat ttgattaaaa aatagctacg cacacttgtg actttagtca tgagttagta
     8821 gctaaattag tcagcatata gggaaactta tatgtagaga taggatagaa cctatccaat
     8881 actacttgaa ttgctaaaaa cccctaaagc taaccaaact aaaacataag g
//
LOCUS       DAELSY010000153         8898 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.153, whole genome shotgun sequence.
ACCESSION   DAELSY010000153 DAELSY010000000
VERSION     DAELSY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8898
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.153"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            397..1872
                     /locus_tag="KPG09_003181"
     CDS             397..1872
                     /locus_tag="KPG09_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG0967761.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1959..2372
                     /gene="speD"
                     /locus_tag="KPG09_003182"
     CDS             1959..2372
                     /gene="speD"
                     /locus_tag="KPG09_003182"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBG0967762.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2402..3253
                     /gene="speE"
                     /locus_tag="KPG09_003183"
     CDS             2402..3253
                     /gene="speE"
                     /locus_tag="KPG09_003183"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBG0967763.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3243..4121
                     /gene="speB"
                     /locus_tag="KPG09_003184"
     CDS             3243..4121
                     /gene="speB"
                     /locus_tag="KPG09_003184"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBG0967764.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4301..4501)
                     /locus_tag="KPG09_003185"
     CDS             complement(4301..4501)
                     /locus_tag="KPG09_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG0967765.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
     gene            4968..6458
                     /locus_tag="KPG09_003186"
     CDS             4968..6458
                     /locus_tag="KPG09_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0967766.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            6716..8212
                     /locus_tag="KPG09_003187"
     CDS             6716..8212
                     /locus_tag="KPG09_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0967767.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACISACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFEFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            8346..8696
                     /locus_tag="KPG09_003188"
     CDS             8346..8696
                     /locus_tag="KPG09_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0967768.1"
                     /translation="MKKIVLKDILFLVTTLSIIVILLFVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKNTAFKTTILSTGKSDCILIEIGDKFIMIDTGEDKNGEQIVDKL
                     KEKEINALDYLIKK"
BASE COUNT         3589 a          944 c         1593 g         2772 t
ORIGIN      
        1 aaaattattt agtccaacta aaccaagaat tttgatatat gaaataagaa taatataaaa
       61 gtttagtaat aaaatactta taaattaaaa atagtaaact caaaagctta ctaaaactca
      121 aaaaagtaaa aaaagcaaaa taataaaaaa taagaaagtt tataaatact aagttttaaa
      181 aaaattaata taaacaacaa aaaaataaaa agtttataaa tattgatata gctaatgtat
      241 atgtttttga gtaacttcaa aaaaacactt gtaaaaatgc tggaatgtta atataataaa
      301 tatcatgagt ttaataaaag taaaaataaa aaatagaaat aataaataat aaatattaaa
      361 aatatttgaa tatagagagg agatgaattt gaaatcatgg atagcgagtt tgcaaatgca
      421 gttgaattaa gctatgagtg tgctccatta ttagagtcgt taaaggaata ctcagagaaa
      481 gatatagctt gttttgatgt tcctggacat gtaaaaaata gaggtgtagc tatattaaat
      541 aaatatctag gtgaaagtct catgaaaatg gatataaatt catcaccaac tatggataat
      601 gtatctgcgc caaatgggat tataaaaaat gcacaagatt tgctagcaca agcatatatg
      661 gctgatgagg cattctttat aacgaatggc acaactcaag caatacatgc aatgattttg
      721 agtgttataa aaccaggtga aaaagtatta cttccaagaa atatacataa atcagtaata
      781 aatgcgttaa tactttgtgg aggaatacca atatttatac aaccagaatt tgatgaaaaa
      841 ctaggtataa gtttaaatat tactcttgaa aaagttagaa cagagataga aaaagattgt
      901 aatataaaag cattattttt ccttaatcca acttactatg gagtttgtgc agaccttgag
      961 agtattatag aactatgcca taaaaataat gttttagtgt tagttgatga agctcatggt
     1021 gcacattttc catttcattt agatttacct ccttctgcaa taagtttagg agctgacatg
     1081 gtagctgtaa gtatacataa aactggagga gcattgacac aatcttcagc actcttattg
     1141 aatagagata atgtaagttt tgaaaaggtt cttcaatcaa tcaatatgct acaatcaaca
     1201 tcagcatctt atttgcttat ggcaagtata gatggtgcta gagttaatct agttgagaat
     1261 ggtgaaaagc aactgtcaaa agcattaaat ctatctaggt atgcaaaatc taaattgaac
     1321 aaaattgatg gaataaaagt tttatctaca gaaatattga aacaaaaagg tgttaaattt
     1381 atagatgaaa caaaattgtg tataaatgta aaagaattaa atttgactgg atttgaagta
     1441 tacgatttat tatataaaaa tttttcaatt caagtagaat tgggagattc atataatata
     1501 ctggcacttg tgtccattgg aacaaataaa tcagatatag atagattagt aaaagcactt
     1561 tctataattg caaagattta tagaaaagaa tcaacattaa atgaatttaa tatggttcaa
     1621 ataaatccaa ttattaaact taatccaaga gaagcttttt atgcaccaaa ggaaagtgta
     1681 gaaatcaatt catgtataga tagaatttgt ggagaatcaa taatggcata tccaccaggt
     1741 ataccgatta ttgctccagg agaattgata acagaagaaa ttatggagta tattattttc
     1801 ttaaaaaata gcaatgcgta tcttacagat gtacaagata aaaacttaga tagaatacta
     1861 gtaataaaat aaattacttt attttgcaag ggcaagtgtt tgttaagtag agtttatcaa
     1921 atagaactta ttaaaagtat aaaggagttg gggttgaaat gaaatttgaa acactaggaa
     1981 gacatatact agtagaatac tataattgtg atgaaaacgt acttaaagac ccacgtctta
     2041 tagaaaattt tatgaatgat tcagcattaa atgcaaaagc gacaattgtg gattctgtat
     2101 tccaccattt taatccttgg ggggtttcag gagctgttat aattgctgaa tctcatttga
     2161 caatacatac atggcctgaa tatggttatg cagcagcaga tttttttact tgtggtgata
     2221 tagacccatg gaaaagtttt gaattattag agcaactttt aaagtctgaa agaagcgagt
     2281 ctacagaaat tccaagaggc ttaactacaa aaattaaaaa atattcaaaa aaagatttag
     2341 gtaagattac tcataaacca gaagcagtat aaaacataca agagaaagtg aggattatta
     2401 tatggaactt tggtatacag aagaatggac agaaaatgta agattttcta taaaagtaaa
     2461 taaacatcta tttgaaggaa agtctcaatt tcaaaggata gatgtatttg atagtgatga
     2521 atttggaaaa tttctaacaa tagatggatt aatgatggta acatataaag atgaatttat
     2581 ataccatgag atgataactc atgttcctat ggctacaaat ttaaacataa agaaagtatt
     2641 agtcataggt ggaggtgatg gaggtacagt aagagaatta agccgttatc ctcaaataga
     2701 aaagattgac atggttgaaa tagataaaat ggttgtagat gtatctaagg agtatatgga
     2761 tatatgctca tgtaaattag atgataaaag agtgagttta tattttgaag atggtgttaa
     2821 ttttgttaag tgtgcacatg ataaatcata tgacctgata atagtagatt caactgaccc
     2881 aataggacca ggcgaagggc ttttctcaac tgatttttat aaggattgtt atagaatact
     2941 gactgatgat ggaattttag taaatcaaag tgaaagtccg tattttgact ttaatgctaa
     3001 agagatgaaa agagctaata aaaaattaaa acagatattt ccaatatcag aagtttatca
     3061 agcacatata ccaacatatc catctggtca ctggttgttt ggatttgctt caaaaaaact
     3121 taatccagta aaaaatcaag atagaaatgg atgggagaaa ttaagtttaa aaactaaata
     3181 ttataatagt gatatacatt taggttcgtt tatgttacct caatacgtga aggagatgct
     3241 agatgaagaa taatttctat catatgaaca cttttatgag tatggacaaa aattatgaag
     3301 aatctaatct tatagtattt ggtgttggat ttgatggaac tacttctaac agacctgggg
     3361 ctagatttgc aagtagctct atgagaaaag aattttacgg tcttgagaca tatagtcctt
     3421 ttttagattt ggacttagag gattataata tatgtgatta tggagattta gaaattagtg
     3481 ttggaagtac agaacaagtc ttaaaagaaa tctatcaaga gacatataag attgttagag
     3541 attcaaaggt accctttatg attggaggag agcatttagt tacattacca gcctttaaag
     3601 cagtacatga aaagtacaat gatatatatg taattcattt tgatgcccat actgatttga
     3661 gggaagaata taataatagt aaaaattctc atgcaacagt aattaaaaga atatgggata
     3721 ttgtaggtga taataaaata tttcaatttg gtataaggtc tgggacaaaa gaagaattta
     3781 aatttgctac agaagaaaaa cacacataca tggaaatagg aggaatagat acgtttgaaa
     3841 atatagttaa tatgctaaat ggaaagaata tttatctaac tatagattta gatgtattgg
     3901 atgcatctgt gtttccggga acaggtacac cagaacctgg tggtgtaaat tatagagagt
     3961 ttcaagagat ttttaagatt ataaaaaact ctaatataaa tatagttggt tgtgatattg
     4021 tagagttaag ccctgattac gatacaacag gtgtatcgac agttatagct tgtaaaatct
     4081 taagagagtt atgcttaata atatctgata aaattaaata gagtatttta tataaatagt
     4141 atatattata atataagaag ttattttaaa aaagttagtt taagtttgac gcttttctaa
     4201 agtaacttct tatttttgta attaaaaata aatataataa acaacaaaaa agagccctcg
     4261 atagctcctt ttctaaaata tatatattta aattaaataa ttatattcta tttacgtttt
     4321 cagcttgagg tcctctatta ccttgagtta tatcaaagct aaccttttcg ccttcttcta
     4381 aagttttgta tccatcattt tgtatagctg agaaatgtac gaaaacatca tcttctccct
     4441 ctacagatat gaacccaaat cctttttcat tgttaaacca ttttactatt ccgtttttca
     4501 ttataagaaa acctccgcat tattaaaaaa tacaaactaa aaaaataagt ctgttctatt
     4561 aatataacat aaattttaaa taaaagcaaa taaaattttt aaaaaaatta attactttta
     4621 gaatttaata aaaattgtta aattatacag tgagaagtat tgtaaaattt gacgaaaatg
     4681 taaatgtaga taataaattt aatgtcaaaa aatgcaacaa aaaggtatgt aagtaaaaag
     4741 taaaattaaa atatataata aaaaatatta tatattagat taaaaagtaa aaataaaaat
     4801 agtgaaattt cagaaatatc aataatatta tataataaaa taaaaaaaca ctatatatat
     4861 tcgataaaat aaaatttaaa catataatat tttatgtata tattaataaa aggacatatt
     4921 gtgttataat ggtatcgtat aaaatcattt acgggggtgg tattaaaatg tttcaatttg
     4981 aaacacaaat acacaaatta agacatgatg ttttaactgc aatagctaga cttggaaaag
     5041 aagataacct taccagagaa gcaatggata atattcagta tgaaataata aaaggtgaga
     5101 aacctacata cagatgttgt gtttataaag aaagagctat aatatctgag agagtaaatg
     5161 taagtatggg gttacaacca ggtcaagata agatagattt agatacaatg gatatagaag
     5221 aagatgaaca aattatatat attttggaag ctgcatgtga cagttgtcca ataaatgaat
     5281 ttacagtaac agatatttgt agaggatgtc tagcacatag atgtaaagaa gcttgtaaat
     5341 ttggagctat aacacatgtc ggaggaatgg cctatataaa tcatgaatta tgtaaggctt
     5401 gtggaatgtg taaaaaagct tgtcaatatg atgccatctc agaggttgtt agaccatgta
     5461 agagtgtttg tccaactaat gccttaggat ttgatagaga aaatatgaaa gctatgatac
     5521 atgaggataa atgtttaaac tgtggagcat gtatgtcagc atgtccattt ggagcaatat
     5581 ctgataagag tttgatagct cctgtagcta gaaagttggt tcaaaaagag aaaatgtatg
     5641 cggtagtagc tccagctata acaggtcaag ttgaagcaaa tgttacatat ggtcaaataa
     5701 aaaatgctat aaaatcacta ggatttgttg atgtattaga agcagcttgt ggagcagatg
     5761 cagttacagt tcatgaaagc ttagaatttg ctgaaagaat ggaaaaggga gaggctttta
     5821 tgacaaattc ttgttgtcca ggatttgttg gttatataga aaaaacatac ccaaatcatg
     5881 taggaaaaat atcttctaca gtgtctccta tgatagctag tggtagattt ataaaaagtc
     5941 atgaaccaga tgctaaagtt gtattcatag gaccatgtac agccaaaaag agtgaggcag
     6001 ctaagaaaga attaaaagga gctgttgatt atgtacttac ttttgaagaa ataatggcat
     6061 tatttgaggc atttgatata gacttggcta agtgttcaaa tgaagatata gatgatgcat
     6121 caatttttgg aagaggtttt ggagctgctg gtggtcttac aaaagctata gaaaattatc
     6181 ttgctgaaaa aggtattggt gttaattttg aacctgtaaa gatttctggt tcaagagaaa
     6241 tcaagaaaac tatgactatg gctaatttag gaaagctaga aggtaatttt atagaaggaa
     6301 tgatgtgtga aggtggttgt ataaatggtt ctggtaaatt cttatcaggt gcaaaggtta
     6361 aagccacttt tgatagaaaa aattctcaat caactaagaa aagtgtctta gcaaatacaa
     6421 agataaaaga ttttagtaat ataaatttag aaaaataaag ataatacaaa gcaacaaaaa
     6481 tacatattgt taatagagtt gttctaagtt gaggtttaac ttttggacaa ctctattttt
     6541 atttatatat ttaattaatg aacaactaaa ataaaaatta catatataat attttgtata
     6601 taacttaaca aagtatatgc ctatgttata ataatttata gaaataaata aaaaatacaa
     6661 tatacataat agtagaaata tagtaaaaag gtgtgattta tagggaggat tacttatgtt
     6721 tgaatttgaa acgcaactaa aaaggttgaa acatgaagtt ttaacagcag tagcaagact
     6781 atctattgaa gaaaatttaa caaaagaaaa tatagaaaaa attccatatg aaataattaa
     6841 aggagaaaag gcgagatata gatgttgtgt ttataaagag agagaagtag ttcttgaaag
     6901 ggcgcaactt gcattaagtt taactccaaa tagtaaatat ggagatataa atccagattc
     6961 agttagtttg gacacagatg agcaaattat atatatagtt gaagcagcat gtgatagatg
     7021 tccaataaat gaatttacag ttacagagct ttgtagaggg tgtttggctc atagatgtaa
     7081 ggaagcatgt aaatttgggg caatttctta tataaatggg agagcatata taaatcatga
     7141 tttatgtaag gcttgtggaa tgtgcaaaag ttcatgtcaa tatgatgcta tatcagaagt
     7201 tgtaagacct tgtaaaagcg tttgtccaac aggagctttg gattttaata gaaacactat
     7261 gaaagcaatg atacatgaag ataattgtat aaattgtgga gcatgtatat cagcatgtcc
     7321 ttttggagca atatctgata agagtttgat agcacctgtt gcaaaaaaac ttgcaaagaa
     7381 agaaaatatg tatgcaatag tagctccagc tataacaggt caaatagata gtaatattac
     7441 atatggtcag ttaaaaaatg ctataaaatc acttggattt gtagatatga tagaagcttc
     7501 ttgtggagca gatgcagtta cagttcatga aagcaatgag cttattgaaa gaataaaatc
     7561 aggagataat tacatgacaa attcatgttg tccgggattt tttaagtata tagagaagat
     7621 gttcccagat gaaaaagata gaatatcatc tacagtgtct cctatggtag caacaggaag
     7681 attagtaaaa aaaatagatt ctgatgctaa agttgtattt atagggccat gtacagctaa
     7741 aaagagtgag gcaacagaga aaagtttaaa agattcagta gattatgtac ttacttttga
     7801 agaactaatg gcattatttg aagcatttaa tatagattta tcaaaatgtt caaatgaaga
     7861 tataaatgat ggttcgattt ttggaagggg atttggagcg tctggtggac ttgccaaatc
     7921 agtacagaac tatattgctg aaaagggact tagttttgag tttgaaccta taaaagtatc
     7981 tggtccaaga caaattaaaa agacaatgac aatggctaaa ttgggaagat tagaaggtaa
     8041 ttttatagaa ggaatgatgt gtgaagaagg ttgtattaat ggagcaggta agtttttatc
     8101 aggtgtaaaa gctaaaaata cttttgatag aaaaaatgga caatctacta aaaagagtgt
     8161 tttatctaat gataagatta aagaatttga agatgtaaat ttagaaagat aattgttttg
     8221 gtacagtcat ttttttatca tgccacaaat atgcctaaaa ttttcccttt ttatatgtgt
     8281 attttacaga gttaaggttg ctatactata aatatgtaat aaataaaatt tataggagct
     8341 tttggatgaa gaaaatagta ttaaaagata tcctgttttt agttactaca ttatctataa
     8401 tagtaatatt attgtttgta acaaatataa ctactaatag taagataaaa tcaacaagtg
     8461 aatcttattt aaaaagtgac aaaaatagtg tgaggcaaaa tggtatccag aggagcacaa
     8521 aaaatactgc atttaagact actatattaa gtactggaaa gtctgactgt atattaatcg
     8581 agataggtga taaattcata atgattgata caggagaaga taaaaatgga gaacaaatag
     8641 ttgataaatt aaaagaaaaa gaaataaatg cacttgacta tttgattaaa aaatagctac
     8701 gcacactcgt gcctttagtc atgagttagt agctaaaata gtcagcatat agggaaacct
     8761 atatgtagag ataggataga acctatccaa tactacttga attgctggaa acccctaaag
     8821 ctaaccaaac taaaacataa ggatgaaata aacctaagtg tgaaagttac gaaagtagaa
     8881 aaaattggtt agatggta
//
LOCUS       DAELSY010000154         8826 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.154, whole genome shotgun sequence.
ACCESSION   DAELSY010000154 DAELSY010000000
VERSION     DAELSY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8826
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.154"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(126..641)
                     /locus_tag="KPG09_003189"
     CDS             complement(126..641)
                     /locus_tag="KPG09_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBG0967769.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(1004..2074)
                     /gene="leuB"
                     /locus_tag="KPG09_003190"
     CDS             complement(1004..2074)
                     /gene="leuB"
                     /locus_tag="KPG09_003190"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBG0967770.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDEIGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTRAMGELIVNEI"
     gene            complement(2081..2572)
                     /gene="leuD"
                     /locus_tag="KPG09_003191"
     CDS             complement(2081..2572)
                     /gene="leuD"
                     /locus_tag="KPG09_003191"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBG0967771.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKEKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(2587..3864)
                     /gene="leuC"
                     /locus_tag="KPG09_003192"
     CDS             complement(2587..3864)
                     /gene="leuC"
                     /locus_tag="KPG09_003192"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBG0967772.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTITKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(3821..5563)
                     /gene="leuA"
                     /locus_tag="KPG09_003193"
     CDS             complement(3821..5563)
                     /gene="leuA"
                     /locus_tag="KPG09_003193"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBG0967773.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIKGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(5687..5933)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6081..6527)
                     /locus_tag="KPG09_003194"
     CDS             complement(6081..6527)
                     /locus_tag="KPG09_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967774.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(6548..7267)
                     /locus_tag="KPG09_003195"
     CDS             complement(6548..7267)
                     /locus_tag="KPG09_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG0967775.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYSGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGTRVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(7385..8659)
                     /locus_tag="KPG09_003196"
     CDS             complement(7385..8659)
                     /locus_tag="KPG09_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBG0967776.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
BASE COUNT         2686 a         1730 c          889 g         3521 t
ORIGIN      
        1 acgctattac gataaatctt tttagtataa ctaatttatt tttatcttgt atttttattg
       61 tatttttgaa ttttattttt tctaaatata aaatttatgt tttaaaatat aaaattttta
      121 tttaatcaaa gttcaatata ttccactttt tctttcgatt cttcaaaaaa ccattcttca
      181 ttagaaaaat caccttctgc aatttgtcca ataggcattt tcatatactt tccagttgct
      241 gtcactgcta tatctccatt tggtagaatt atttctcctg taccttcaaa taattttctg
      301 ttttctttag ttattcttcc aacaactttt atagtagcat ctgttggtac tggtttctta
      361 tatttcaact ctaaagatac tgttactccc catatttggt catcatttat acttacagct
      421 cttccaatag tctcatctaa tattgcagca gacatacctc catgaactct cccaggataa
      481 ctttgatgcc aatcctttgt attacaaatt gatacaagct ctccattttc aagttcataa
      541 aaatctgctt taagtcctag ctcattttgt gttccacaaa ctaaacaaga tttactacta
      601 ttttgcttct tcaaaacttt atactttgcc aaaaaatcca tctcctaacc gacattagta
      661 gtcgcttaat attaaataat tctaaaatat attatataat attaataaaa tccctgcaaa
      721 cttctaataa taaaaatagg ctacttcaat atgataagtt taatttaaag tattattttc
      781 caaaaaataa ctttatcaaa atagaaactc aaagtagata taagcctctt tttcgctatt
      841 aaaatagtaa aaatatattt gacttaatat aaaaaagagt gtctcattaa ctaataaagt
      901 ttattttgag acacccctca taaaacaaat tatactacaa tagattttta ctaattctac
      961 aagttatata tttaaattat attattcaaa ttaaactata attctaaatt tcatttacta
     1021 ttaattcgcc cattgctctt gttcctacaa catttcccac accattatag atatcaatag
     1081 ttctataacc tttattgagt acactttgta cagcatcttc aatacactta gcttcttctt
     1141 ctaagttaaa actatgtctt aacatcatgc ttacagatag aatagttgca attggattta
     1201 caatatcttt tccagctata tcaggcgcac ttccatgtat tggttcatac ataccaaaac
     1261 tatcttctct aagtgatgct gatggtaaca tacctataga acccgtaatc atagatgctt
     1321 catcagatat tatatctcca aatatattat tagtcactat tacatcaaat tggctagggt
     1381 cttttacaac ttgcatagca gcattatcaa catacatatg tgacagttct acatcctcaa
     1441 actctttagc taagtcttcc attatacttc tccaaagtct tgaactctca agcacatttg
     1501 ccttatctac acttataagt tttttatttc tatttctggc tatttcaaaa gctctcttac
     1561 caattcttct tatttcattt tcgttatata tttctacatc ataagcattt tcgataccat
     1621 caatgatttt tcttccacgt tctccaaaat aaatgccccc tgttaattct cttacaacaa
     1681 caaaatcaat gcctttctca actatatccg ttctaagtgg tgaagcttct tttatacttt
     1741 catacattgt agctggtctt aaatttgcaa ataaacccaa tgactctctt aattttaaaa
     1801 gaccagactc tggtctttta tctcccttac atttatccca ctttggtcct ccaacagcac
     1861 caagcaatac agcttcgttt tttcgacaag tttcaacagt tttattaggt agaggttctc
     1921 caatttcatc aatcgcgcat cctccagcta atacatattc acaatcgaat ctatgattaa
     1981 atttacaaca aattttattt agaaccttta taccttcatc tataatttcc ggtccaacac
     2041 catcaccttt tatgactgct atattacaat tcatactcca ctacccccta tttgctttta
     2101 ttgagttaat taagccttca tttaatataa tattttgcat aaactcagga aatggttctg
     2161 ctttatattc tttttctttt gttatatttt ttatcacacc tgtagaaaaa tctacttcta
     2221 tattatcccc ttcatctatg ttattggcag cttcttcaca ttcaagtata ggaagcccta
     2281 tatttataga gtttctaaaa aatattctag caaaagtaga agcaatcaca caggatactc
     2341 cactttcttt tattacaatt ggtgcatgtt ctcttgatga accacaacca aagtttttag
     2401 ttgcaactat aatatctcct ttttttacct tagaaataaa tttatcatca atatcctcca
     2461 tacaatgcgt tgctagttct ttataatcag ctatatttaa atatctagct ggtattatta
     2521 catctgtatc tatattgtca ccgaatttaa aaacacttcc attggcaatc ataaatttta
     2581 cctcccttaa attatttctg atggttttga tatttttcca gttatagctg atgctgctgc
     2641 tacagctggg cttgcaagat acacctctga atcaacatgc cccattcttc ctacgaaatt
     2701 cctatttgtt gttgaaatgg atttttctcc tgctgcaagt atacccatat gtccccctag
     2761 acatggacca caagttggtg tagaaactgc tgctcctgcc tctataaaag tggtaatata
     2821 ccctttttct atagcttcaa gatatacttt ttgagttcca ggaaatatca taactctaac
     2881 tccatctgct acttttttgc ctttcattat ctcagctact acttcaaggt ctgatattct
     2941 tccatttgta caagaaccta ttacaacttg gtctatcttt atatccttat ctatctcatc
     3001 tactgttcta gtattttcag gaagatgagg aaaggctact gtttctttta gttcggctaa
     3061 atctatttcg tatacttcat catagactgc atcttcatct gcttcatata tatttacatc
     3121 ttttgttata gttgaacact tatgtgaatt tatatattcc atagtcttat cgtctactgg
     3181 aaagattcca ttttttgctc ctgcttctat tgccatatta caaatagtaa atctatcatc
     3241 catagataag tattctactc catctccaca aaattccatc gacttgtaaa gtgctccatc
     3301 aacacctatc tcccctatta tatgaagtat tatatcttta ccacttatcc atttatttgg
     3361 tttattcttc aaaacaaatt ttattgctcc aggaacctta aaccaacatt ttcctgtagc
     3421 catacctgct cccatatcag ttgaacctac accagtagaa aatgctccta atgctccata
     3481 tgtgcaagta tgagagtcag cacctataat tacatctcca gcagttacta tacctttttc
     3541 aggaagaaga acatgctcta ttcccatatc tcctacatca aaaaaattta caactccttt
     3601 tgattttgcg aaacttcttg taaatttaca ttgttctgca ctctttatat ctttattagg
     3661 tgtaaagtgg tctaaaacca tcactacttt agtcttatca taaacatctt caattcctaa
     3721 tttttcaaat tctctaatgg ctactggtga tgttatatca ttacctaaaa ctacatctaa
     3781 atctgcttcg attaattgcc ctttcttaac ttcatttaat ttagcatgtt tggctaatat
     3841 tttctgcgtc atagtcatcc ccatttttta tttcctcctc aatataactt ttatttattg
     3901 cattcataag tgcattaata gctgctgtat ttatgttttc cgatatactt acaccaaaat
     3961 agacttcatt attgtgctct attcctacat aagctacccc tcttgaatgt gacccttcct
     4021 ctaatgcatg ttcccagtaa tatctaaatt tataatcttt catgccacat tgctttagtg
     4081 cattattaaa tgaatctaca ggtccatttc ctataccaac aatcctttgt tcatttccca
     4141 tataacttat tcttgcagtc atctcaatca tgttagtatc attagaatca tcattttcat
     4201 agtttaattc atccattgat tttattttat acttcttaac tttataaggg ctctcaactt
     4261 caatatattc tttcttaaat aaattaaata ttgcttcact actaagttct tccccaattc
     4321 tatcagattc cattttgact acatttccaa aatcagaatg catattctta ggtatcataa
     4381 atccataatc tgtttccatt atataaacag ctcctccttt tccagattgg ctatttatcc
     4441 ttataatttc tttgtattct cttccaatat catgagggtc tattggtaaa tatggaactt
     4501 cccagtaatc attatctctg tttttcattg ctatcatgcc ttttctaata gcatcttgat
     4561 gtgaacctga aaaagcacaa tgtacaagat tacctgcata tggatgtcta tcatgtacca
     4621 taagttttgt acagtcttca tatataccaa ttatcttatc tatattgcta aaatctagct
     4681 ctgggtctat tccttgtgta taaagattaa gtcccacatt tactatatcc atatttccag
     4741 ttctttcacc atttccaaat agtgttcctt caagtctatc ggcaccagcc aataatccaa
     4801 gttcacttgc agccgtacaa gttcctctat cattgtgagt atgcaaactt agtataatag
     4861 aatccctaca acttatattt ttacaaaacc attctatttg gtctgcatat acgtttggtg
     4921 ttgccatttc cactgttgat ggaagattta ttattacttt tttttgagga gttggtttcc
     4981 aaacatctat cactgcctca catatttcaa gtgcataatc catctctgtt cctgtaaaac
     5041 tctctggtga atattcaaaa gtaaattctg tttctggata tttttttact tcttcattaa
     5101 ataattttgc tccttcaact gctatatcta ttatttcttg tttactcata ttaaaaacca
     5161 catctctttg aagaacagat gtagagttat ataaatgtat aattgctttt ttacatccct
     5221 ttattgattc aaatgttttt tctataagat gagccctaga ttgtgtaagt acttgtatag
     5281 ttacatcatc tggaatcata ttctcatcta ttaattttct taaaaatgta tactctgtat
     5341 ttgatgctga tggaaatcct acctctattt ctttaaatcc tgtatcaata agcattttga
     5401 acattctgac tttttcattt acactcatag gcgttggcag tgactgattt ccgtctctta
     5461 aatcgacact acaccaaatt ggagccttaa atatttcatt atctggccat ttacgattta
     5521 ctatttgcat tttatcatat tttttatact ttccacactt catgttattt cccccttttt
     5581 ctgttttttg tattcagtat accttatatc gacgcaatcc tgcacacttc ataaggataa
     5641 atgtcaattt tatcattaga ataaaaaaaa cctcatctct aataaaatta gagacgaggt
     5701 tttgctcgcg gtaccactct atttagcttt ctaaaaagct cccttagtaa tagccaatac
     5761 aataattata atgactatat ttcctataac gtggaattac tccgattatt ctaagattta
     5821 aatcttagaa aacagctcct agacaagttc agaactatca actactaaat cacaccatcc
     5881 tttagctctc tgaaagtttc attgttctta ctacttctat tcatcacttt tttaatcatt
     5941 taaattttct aaatattttg atttaatagt tattatatac acattaaaat gtattgtcaa
     6001 caaaaaaaat aattatctca attttttatt tttgataagc aattctataa aacatgctaa
     6061 ctattgaaaa cagtatgata ttatgcctta aactttttca tcttttcatc tatgatattc
     6121 aataattttt tttcatcttc caaactcaat gcccttaatc taattttata attagattcg
     6181 tcagtgtata tatttaatat attttttact acttctattt tttctattat cttataatta
     6241 aacttttgac tttgtttaga ttttatactg aatatacatt gattatttat taatattgaa
     6301 tgattatcga gagtaatata tacttttccc tcatatttat ttgaaaaatc acgaccttgt
     6361 ttaaatagaa aaactgctgt tattatacat aaaatatata atattgtcgt tgaatatata
     6421 aaaattgtag ctaaaattat aagtgctaaa accatatata tagtcattcc ttttttaaaa
     6481 taaccattgt cataataatt catatagata aacttatttt tactcaaatt ttttcactcc
     6541 caagattcta aaaatttata tcaaaaaatt ctataatttc atcttcatga taatttatga
     6601 ctaattcatc tggaaaatct actgcttcta aaactttttc tgcattttca aattctccta
     6661 tagaataaca tatatgagag tcagtaccaa gaattactct tgtaccatat gttttacaca
     6721 atgtcaacat atgacttaca ttttcccaag tacccgttct atgagagttt gaacttaatg
     6781 atgaattatt tatttctaaa aggatatttt tatctttagc tttttttact ataggttcat
     6841 aatctaaagg atacctactg tcatctggat gtccaattat ttttacctta ggtttatcca
     6901 taacttttaa aatagcattt gtattttctt cttttgtacc agaagctata caaggtctat
     6961 gcaaacttgc gattatataa tccaaatgtt gcaacatatc ttcttgaaca tctaaattac
     7021 cactataatc cattatattt ccttctattc ccctaagaat ctttacacct tgcacttcac
     7081 gtggaagcaa atgcaaatta taaaaataga atggatgtgg tcctcctggc atattaggtc
     7141 cgtggtctga tattcctaag tattttatat tcttcttact cgcctcttcg acattttcct
     7201 tgaaagtact atgtgcatgt ccacttatta gagtgtgaca atgtacatcc ataatagact
     7261 ttttcattaa atttcctcct aatattttac ataaattcgt gaaaatagct aaagggctat
     7321 ttcaaaacta aagctgtcat aatagacagt gtaacgtttt aaaacagccc cattttcaat
     7381 tattttacac ttctaaatct ttaaatttat ctttaaataa ttctccaata cttcctaaag
     7441 attcctctac ttctaggtat tctgttatat cctcttctgg ctctctagtt gcttctttta
     7501 ttgaaaccaa cattctttta gtctttggtt taaaatcaag aatcttaact ttaacctcat
     7561 ctccaatatt aacaacatta gtaactttag ccactctagt atcactcaat tcagatattg
     7621 gtaaatacgc atctacacct tctttaattt gaacaaaagc ccctttttct attattctaa
     7681 gaactttagc attgacaaca tcatcaattt gtacttcttt tgctattaaa tcccaagggt
     7741 cattatttat gtcttttaat gccaaggcaa tttttttagt ctctttattt acatccaaca
     7801 cataaacttc tacttcttgt ccctctgaaa caacatcttc aacactttca actcttgtcc
     7861 aagctaaatt atttatatgt gctaatcctt caatcccacc aatatccaca aatgcaccat
     7921 aaggcattat ctttgtcacc ttaccatctc ttttttgtcc aacttcaaga gaattaaata
     7981 agtcttcttt agctgctttg atacgagctt tttcttcttc tctttcttgc ttaattcttg
     8041 ctctttcagc ttctttttct gccttaattt gagctcttct ttcttctctc tctttattta
     8101 tacgctcttg taatacatct ctatgagatg ctacaagtct attttttcta acatccaatt
     8161 cttttatata aacttctaag ttaagaccaa caaatttaga tgtatctgta ataaattttg
     8221 tatcaagttg agaaattggt ataaaaaatg tatatgtatt gtaattagca aatacacctt
     8281 tatctatatt cttttcaaca tttacagtta ttattctatg ttcatcataa gccttttgta
     8341 actcggcaaa atcatttctt ttgtcaagtt gtaatttaga taaagttatt gtaccatctt
     8401 tttgagatac ttttgttatt acagcagtta tttcatcacc tatgtggtat atatcttcta
     8461 tatattgatt tttttctatg ttcaattcac ttataggtat tattccatca aacccatagt
     8521 ctaatcctaa ttgtacttca tcatttctca ctgcagttat ttttccagtt gtcaattctc
     8581 caactttcac tttgctgaat acttgttctt gctggtcaag taattcttgc attgttaaat
     8641 cattttccat tataaacatc ctttcctctt attgattatc atatctgtaa atattatacc
     8701 attaatattg caaatactta catttttctt actataattt ttaaaataaa ttcaacttca
     8761 tatatttata cattatagaa aatatcttat taaattaaaa tacaaattct aatttttatc
     8821 taataa
//
LOCUS       DAELSY010000155         8643 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.155, whole genome shotgun sequence.
ACCESSION   DAELSY010000155 DAELSY010000000
VERSION     DAELSY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8643
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.155"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            230..1309
                     /locus_tag="KPG09_003197"
     CDS             230..1309
                     /locus_tag="KPG09_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG0967777.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDEINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYMKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRRH
                     YPGIHSITIIINGVEKGKLDFELGV"
     gene            1633..2001
                     /locus_tag="KPG09_003198"
     CDS             1633..2001
                     /locus_tag="KPG09_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG0967778.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2118..2921
                     /locus_tag="KPG09_003199"
     CDS             2118..2921
                     /locus_tag="KPG09_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0967779.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFMPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPSELNQIMDIFIKQLNI"
     gene            3306..4196
                     /locus_tag="KPG09_003200"
     CDS             3306..4196
                     /locus_tag="KPG09_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0967780.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSARFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTIQNKDSCEKLLREECARYGILTD
                     MKEIIKAYKRSESYIQIKFF"
     gene            4659..5102
                     /locus_tag="KPG09_003201"
     CDS             4659..5102
                     /locus_tag="KPG09_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0967781.1"
                     /translation="MIEIKTIEINKKSQIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            5274..5444
                     /locus_tag="KPG09_003202"
     CDS             5274..5444
                     /locus_tag="KPG09_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967782.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            5995..6903
                     /locus_tag="KPG09_003203"
     CDS             5995..6903
                     /locus_tag="KPG09_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG0967783.1"
                     /translation="MTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFINDI
                     LKDAPYANRNIKPFTHIKRIIKAETDPTINYIGEKRPRLYILAEDEESNHINIEMQRA
                     LEDDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLTM
                     DGHPNIYSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIKR
                     ALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGVEKGKREEKFNIIIKLKNK
                     GYNLSEICDIIDDLNKSEVEKIYNQN"
     gene            7083..7571
                     /locus_tag="KPG09_003204"
     CDS             7083..7571
                     /locus_tag="KPG09_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBG0967784.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            7657..8064
                     /locus_tag="KPG09_003205"
     CDS             7657..8064
                     /locus_tag="KPG09_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967785.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            8065..8283
                     /locus_tag="KPG09_003206"
     CDS             8065..8283
                     /locus_tag="KPG09_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0967786.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
BASE COUNT         3400 a          855 c         1526 g         2862 t
ORIGIN      
        1 agtaaagtat aaaattctat tactataata aaagatagaa ctagaagtct ttatgaaagt
       61 gggaaaagta tttatataaa taaatttata tggtttatag tttaacagta ggaaatatcg
      121 tatgagtgag aatatcgtat tttacataaa gatttagcaa tatgacacac tgttgtcgtg
      181 ttctgtagga tatgattaat aaatcagata tgaaaggaga gtgagtaata tgccagaatt
      241 actaaaaaat atgtataatc gtgagtcact ttatgaagtt gcagtagcta tccaatcagt
      301 ttacaactca tttaaggtag acgagttcat aaaatctaca atggatgaga catggaataa
      361 cttagaatta aaagccagat gtcgaaagat aagtatgagt ttaggaatgt atttaccaga
      421 agactataag gaagctcttt ccattcttga aaaatcagtt acaggttttt attttgcttt
      481 tttctttcca gattttgttg aagtttatgg gcaggacgaa ataaattggg atttatctat
      541 aagtgcatta gaaagaaata ctgagtattg gtcttcagaa tttgctgtaa gagcatttat
      601 catcaaggat gaagagcgta tgatggcaca gatgcgtaaa tggtctaagc ataagagcga
      661 acatgtacga agacttgcaa gtgaaggttg tcgtccccag ttgccttggg gacaagcaat
      721 atccaaattt aaaaaagacc ctacacctgt actgcctata ttggagcaac ttaaaacaga
      781 tacatcaacg tatgtacaaa agagtgttgc taataatcta aatgatatct caaaaacaca
      841 tccagattta gtcataagca ttgcaaagga ttggtacggt aaaaataaat ctactaactg
      901 gattgtaaag catggttgta gaactctact aaaaaaaggc aatcgtgatg tattagccct
      961 ttttggatat gatgatacta cttctataaa tcttcaggac tttactttag aaacaacgtc
     1021 tatttctatt ggagaggatt taactttttc atttaatatt ttagcaaaaa aagcgacaaa
     1081 aacaaggctc gagtacggaa ttgattatat gaaatctaat ggaaaaagga ataggaagat
     1141 atttaaaata tcggaggttt cattaaaaga aaatgagaaa aagtcatata tgaaaaaaca
     1201 ttcatttgca gatgtaagtg taaggagaca ttatccaggt atccactcga ttactattat
     1261 tattaatggg gtagaaaaag gcaaacttga ttttgaatta ggtgtataat tttactaaca
     1321 gcataaccta tatcagatac tttgctaaac ctatatcgta tactctaata aatctatatt
     1381 aggtagttta ctacttgttt actgaattta aatacaaaga aatacaggtg ttaatccctg
     1441 cccattaaag gaatatgagc ataacacaaa agtagaactt atcaattctg ttacgggtat
     1501 agtagtaaat ccatagaatc acaattagaa gatgaagtgt aaaaaataag ttagtgttga
     1561 ttttagttgc tagtgatatt gaatagttat acaatagcca tataaattag aatttgtaga
     1621 ggagaaatgt aaatggatgt atatagtttt tggaatgaca ctttggcaca agatgctgtt
     1681 aagataagaa aatattttca tgaagatgct tatattaatt ggcatgatac aaatgagcat
     1741 tttactttag aagaatttat tattgcaaat tgtgagtatc ctgataaatg gaatggtaaa
     1801 gtagaaaggg tgctagaatt tggaaattta gtaattacag tgacacatgt ttatgcaata
     1861 aataaaccac tatcatttca tgtcgtttca tttatcagcc ttaaagatga taagattatt
     1921 tctattgatg aatattgggg agaggatgat gatgtgccac agtggagatt agataaacat
     1981 attggaaagc caataaaata gtgtttgttc tgttttataa ctgaaatttt gataacattt
     2041 gatattttga tttgtcattg gtcaacatag tcgttagtgt aataatttaa aaaagtagaa
     2101 tttggagagt gagcactatg gaagaaaaat acatcttatc tgaccgaggt aaagtatatt
     2161 attggataaa agaaagtaat agtgtgtttg caaagaactt aatttttttg ccaggactaa
     2221 cagcaaatca tcatcttttt gatttccaga tacagtactt ttcaaaaaat cataatgtat
     2281 tggtatggga cgctcctgct catggaaaat caagacctta cttagatttc tcatattcta
     2341 acttggtaga agaattgaaa gtaattttag ataaacaaaa tatgaaaaag gttgtactta
     2401 tagggcaatc ggcaggggga tttgtggcac agtcctttat tgtaaaatat ccaaatatgg
     2461 tagaaggatt aatgatgatt ggaacttgtc catatggaac tgactattat agtaagtcag
     2521 atattttctg gttaaaacag actgaatgga tgttaaagtt attcccagat aaattgttgc
     2581 gaaacataat atctaagatg tgtggagcta cgtctgaagg cagaaggagt atgatgaaaa
     2641 tgttagaagg ctacgaaaaa aaagaactgt gtcatctcat gtatctggga tttgctgggt
     2701 tcatgccaga aattcaagac ttaaatatac attgtccagt ttgtttggtg gttggagata
     2761 aagacaagac gggtaaagtg agaaaatata atgagcagtg gcacaataag actgggtatc
     2821 cattgcacat tataaaaaat gcttcacata atgtaaatgt cgataaacct agtgaactaa
     2881 atcagattat ggatatattt attaagcaat taaatatata aaaactacat actcattagg
     2941 taaattatgt agtatatgtg tttacatatg gttatagaaa taaatatgta ataaactact
     3001 taacacttaa aaagtggatt tgtccagagc ataatacaaa gcatgatata taaattttta
     3061 agcgaaatac atcaaaagaa gtattagaat tagtggagat aaaacagcta ttagagatta
     3121 taaataaata gttttaaagg tactacgaat atagaggaag gtaaatcata cgttattcaa
     3181 ggattataaa tgagtagttt aaaagtacta tcggataggt ctctgcatat ttaaacctta
     3241 gtaaaggtag ttaaagtagt agtttaaaaa ctaaaaaatt atataaagga attaggtata
     3301 agaaaatgat tgagagtata ccagcaaaac aaattttaca aaaagtgaaa tttgacaata
     3361 caagatggtt tggaattgac tataatatga atctctatcg tggttgttct catggatgta
     3421 tatattgtga tagccgtagc acaatataca acatagagaa ttttgacaaa gttagatata
     3481 aagaaaatgt gatagagatt ttaagtaaag aattgcgttc aaaaagaaaa aaaggggtag
     3541 taggcattgg agctatgtct gacacatata atccttttga aaagcaactt tgtattacaa
     3601 agcaagcact agatttgatt tcggaaaatc attttggagt gtctattgat actaaaagta
     3661 gtttggtggt tagagatata ccgattttgc agaaaattaa aaaaaataat tctgctattg
     3721 taaagttgac tataaccact gcaaatgatg aattatcaaa gaaaatagaa ccttatgtaa
     3781 atccaagttc tgcacgattt gacgcagtga aagaacttaa tgattctgga attttttgtg
     3841 gcatattatt gactcctatg cttccatttt tgactgataa taaagatgaa atacgagcaa
     3901 ttgtagaaaa agcacacaag gctaatgcaa agtttattta ttgcatgtat ggtgtaacaa
     3961 tgcgtagtgg tcagagagag tttttttatg agcatttaag agatatatca ccaaaattag
     4021 tacttaaata tcagaaaaca tatggtttaa actatgtatg tactatacaa aataaagatt
     4081 cttgtgagaa gttgttaaga gaagaatgtg cacgttatgg tattttgaca gatatgaaag
     4141 agattataaa agcgtacaag aggtcggagt catatattca aataaaattt ttttaagata
     4201 ggctttggat gagatataat agtggtgatt gatgtgttga tattatagtt gattgttaga
     4261 gaggatatta tagatgaata ttaatattga tattctaggt aattgctaga actaatatta
     4321 tagatgaata ttagtattga tattctaggt aattgctaga gttagtatta tagatgaata
     4381 ttagtattga tattctaggt aactgctaga gctaatatta tagatgaata ttagtattga
     4441 tattctaggt aattgctaga gctgatatta tagatgaata ttagtattga tattctaggt
     4501 aattgctaga gctgatatta taatttatta ttaatattaa tattctaggg aattatcact
     4561 atattttaaa aagtaaaaat atatatcaaa atgaactaaa taagtactgt aagaagtttg
     4621 ttaaattaag gtttgaataa agtgagggtg gagaacttat gatagaaata aaaactatag
     4681 aaataaataa aaaaagtcaa attgaccttt taatgttagc cgacccatgt gaagaaatga
     4741 ttgataaata tctcaataaa ggaactatgt acgcattgta tgataataaa gaacttactt
     4801 gtatagcagt tgtaaatgaa atttccaaag aaatatgtga attaaaaaac attgctacat
     4861 atgagcattt tcaaaatatg ggttatgcct caaagatgat ttatcatctt ttagatgttt
     4921 atagtaaaaa atattcatca atggttgttg gtacatctga aagcggagtt cctttttatg
     4981 agaagtttgg atttgtttat tcacataaaa ttaaaaactt ttttgtagat aattatccag
     5041 aaccaatctt tgaaggagaa ttacaatgtg ttgacatgtt gtatctatca tataactttt
     5101 agataggata atatttttag gtagatataa ttaattaatt catataatta tcggaattat
     5161 aatttataaa ttatgtataa taagagtata atttatttag aagctgttat attgtattat
     5221 attcaaataa gatatataat aatttgttta ttttgaattt tggaggatta attatggatt
     5281 caaataataa tactataaaa tcaactgtta aaaagggtat ttcttttggt tcttgtttag
     5341 caatgattat ttcttatact gcatggaaat ctattccatg ggctattttt catggcttaa
     5401 tgagttggat atatgtactt tattattggg ttaagtatgc atagattaaa ttaataaata
     5461 agtatgtaat attgtaaagt ttaagaaaca aaatgtaagg gagtgtctcg aagtgaactt
     5521 tttagtttat aaggcactct tttttgtatt aaataaaata tattttcatt acttaaacaa
     5581 tgaaaaagat gagtatctct attttaatac atacccttat attcattcat ttatcaacaa
     5641 tagttttaaa aagaaagcta tctaaaatct attgtaatct aagtgcttct ctttaaagtc
     5701 ttaactttag taatgatata taagtttata aggttcttat ttcaataatg caaattttac
     5761 ttagaagttc agactttggt atgaagaaat gggtaataat aaaaaagagg tgttgatatg
     5821 aaagaaaaat tagaagaaaa atctaagatt attaaaacat caaaagagta taaacgaact
     5881 ataattgaag aaaaggacag agaatgaacc aaaaaattaa atgacatact tagtagaggt
     5941 gatttttcta atgaaaaatt agataaaatc aaggatttaa ttccaaaaga aatattgact
     6001 agtgaaaatg ttggagaagt tgtaactgaa gatgggtatg ctaagcctga gtatgatgag
     6061 gtatttaaat cattgtttac tttaaataat gattacgatt tacttgcaaa ttttataaat
     6121 gatatcctca aagatgctcc atatgccaat aggaatatca aaccgttcac tcatataaaa
     6181 agaataatta aggctgaaac cgacccaaca attaattaca ttggtgagaa aagacctaga
     6241 ctttatattt tggctgaaga tgaagaaagt aatcatataa atattgagat gcaaagagct
     6301 cttgaagatg attatttgga aagagctgaa tattatctta gtcgtgtaca tggtagaaag
     6361 ttagaagaag gtaaagagta taaagaaatt ggaaaaactg ttggaataca tatactaaat
     6421 catgtaaaat ataatcacat agaagattat gtaaattgcc tgagattaac aatggacggg
     6481 catccaaata tttattctag taaaactgca ttatatttta tagaacttcc aaaaattcgt
     6541 aaatccagtt gtatagcaaa cagagttcta atatggggta aacttattga taatccttct
     6601 catattgata ttagaatact atctaaaaca gattatgtta ttaaaagagc acttgataga
     6661 ttaaaagaat taggcagtaa tgaggactat ttattaaatt taaagagagg tgcgtatatt
     6721 atgaataaaa gtagaaattt taaagaagaa atttttcaag aaggtgttga aaaagggaaa
     6781 agagaagaga aatttaatat catcataaaa ttaaaaaata aaggatataa tttgagtgaa
     6841 atatgtgata ttatagatga tttgaataaa tcagaggttg aaaaaatcta taatcaaaac
     6901 taatactata attgagatgg cactagataa atataaagct tcctctttta aatttaaatt
     6961 aatataaaac cttgttattt aactatagta gatacaaaga gatatataag ctaaggagat
     7021 gattcaaaat aaaagtttga actatctcct tcttatagaa aaacaaaggg gttgatttat
     7081 atatgaaatt ttatactata cagactttag aattttggaa caacaataaa aacaataaat
     7141 atctatcaaa tgattattct tttatattag aagactttga tattccatac aaatggatgt
     7201 atagtcaaat gacaaataga atatatgaat ttgatgatag tatgatatgg gtttggacga
     7261 agagacctga tttaagatgt agtggttatt tagaaaaagg aaaaaaaggt gttttacttg
     7321 aaatagaaat aagtgaagac aatgtgttat tatcagaatt tgatttatgg catttacctt
     7381 taatggatat tactgtagag ctttatgaag atgaacaaat tgaaaagaag gtaagttggg
     7441 aaagaatatt tgacttagaa ctgtgttcaa aattaaatga agttaatata gaagatttga
     7501 cattgcaagg agtgacatct aaagtgcctc tatctaatgt aaaattaata aaagaatttg
     7561 ttgcaaaata atatttttat tgaaatttat tcaaaaaagt tatatattta taacaagagg
     7621 tgttataaat acataacttt ttaaggggga ttttatatgt ctaaacaaca aactggtatt
     7681 aaatcaacac ttatttcaga aatattggga atttcttatt gtattggagc tacattagta
     7741 aataagtatt tttttaaagg aagtagtatt gaatatcttc ctataattat ttgggtatta
     7801 gctatgggag ctcagtgggt ctataataaa aagtatgatg atttaattga tgaggcatca
     7861 aaatgtatac tatcaagagt taatgatatt gcaataaaag tattgttttt ttctgtagct
     7921 atagtatcta tatttcttgt aactttggac ataaatgtgt caaatttaaa tataggaatg
     7981 tctttattag tcattttgtt tattcaatcc ttgttgaagt taattctatt tatatatttt
     8041 gacagaaagg ggatatacaa ttaggtgtca gtattactaa agacaaaaat taaatcctat
     8101 agagaaaaga atcttatgac acaaggtgaa cttgcaaagt cagtaggagt aagacgtgaa
     8161 actattgtac atttagaaaa tggtcgctac aatccttctt taaaactggc aatggatatt
     8221 gctaaggtat ttgatacaac tgtagaagag ttgtttgaat ttattgaaga aaataatatt
     8281 taaatttcat gtattactta tgcattagag aatgaattaa tagataaaaa attcaagttt
     8341 atgtattaga gaatgaatta attataggta aaaaaccaag cttatagatt agagaataaa
     8401 tgacttatag atgaaaaacc aagctcatag attaaagaat gaatgattta tagatgaaaa
     8461 agaaagctta tagattagag aatgaattaa ttataggtaa aaaacaagct aatagattaa
     8521 aaaatgaatg atttatagat taagaagtaa gcttatagat tagagaataa atgacttata
     8581 aataaaaaga aagcttatag attagagaat gaattaatta taggtaaaaa acaagcttat
     8641 aga
//
LOCUS       DAELSY010000156         8567 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.156, whole genome shotgun sequence.
ACCESSION   DAELSY010000156 DAELSY010000000
VERSION     DAELSY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8567
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.156"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(92..322)
                     /gene="dltC"
                     /locus_tag="KPG09_003207"
     CDS             complement(92..322)
                     /gene="dltC"
                     /locus_tag="KPG09_003207"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBG0967787.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(341..1489)
                     /gene="dltB"
                     /locus_tag="KPG09_003208"
     CDS             complement(341..1489)
                     /gene="dltB"
                     /locus_tag="KPG09_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBG0967788.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKHISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(1489..3003)
                     /locus_tag="KPG09_003209"
     CDS             complement(1489..3003)
                     /locus_tag="KPG09_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBG0967789.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVIFN
                     FSDERNFGDINVIDMDKLNDIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(3124..4314)
                     /gene="dltD"
                     /locus_tag="KPG09_003210"
     CDS             complement(3124..4314)
                     /gene="dltD"
                     /locus_tag="KPG09_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBG0967790.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGINPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(4602..5621)
                     /locus_tag="KPG09_003211"
     CDS             complement(4602..5621)
                     /locus_tag="KPG09_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG0967791.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLHGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQAITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(5797..6336)
                     /locus_tag="KPG09_003212"
     CDS             complement(5797..6336)
                     /locus_tag="KPG09_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBG0967792.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPILVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(6437..7117)
                     /locus_tag="KPG09_003213"
     CDS             complement(6437..7117)
                     /locus_tag="KPG09_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBG0967793.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(7146..8435)
                     /locus_tag="KPG09_003214"
     CDS             complement(7146..8435)
                     /locus_tag="KPG09_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG0967794.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         2829 a         1518 c          828 g         3392 t
ORIGIN      
        1 ttatcattaa aaaagtccat caaatattag tatcagatgg actttgtaaa attttatttt
       61 ataaattaat atttaaagtc taactactaa tttattttct tgtctccaag aattttacta
      121 aattatttac agtagacata tcttttctct ctaaatcagt tggttgaagc tctatcccta
      181 atctattttc aatttcaagt aaaacttcaa ttatagctaa tgagtctaaa agttccgtct
      241 caaataaatt aaggtctaaa tcttctctta tctcatcagt ccctaataca tcttcaaata
      301 tttctataac tgtttcttgc ataaattttc ctccttaaaa ttaagctaaa taacctgaga
      361 atatcagcaa tccaaaacac acaatatgga atgtaatgaa aatctgcact ctctcgaacc
      421 atttatcttt tttatgtttt ttataaaatt tagattttcg ctgatatata tcagttaaca
      481 caagtgccaa tccttggtat acaccataag caacataata ccaagttaga ccatgccaaa
      541 atcccattgt tatcatggtt atcatctgag ccacatgagc agcagtagtc cttttcttaa
      601 atcttttctt tctcattgat gacataacaa atctagaaaa aatataatct ccaaaccatc
      661 ttgaaagact tatatgccat cttgtccaga actctttcat atctttactg ataaatggct
      721 tgtcaaagtt tataggtact tgtattccaa atatgtaccc tgtacctata gcaaataaac
      781 tatatcctgc aaaatcaaag aacaggtata aactatatgc gtacatataa cttaaaatat
      841 ttataaaact catatcttta ggtattcttg atacccaata tgtatttatc aaaaatgcta
      901 taacaaactt ataacctact cccatgacaa tattttttat tcctggcagt aagtattctt
      961 ctatatactc ttttctagag atgtgttttt ccaagtcctg ttcaaatctt cttgacctat
     1021 caataggtcc agaacttaaa gttggaaaaa ataacataaa ataaagcatc tttgaaattt
     1081 taacttcttt tatagctcca tcataaattt caataaccat ttgtatagtt ctgaaattta
     1141 agtatgatat tccaataaat ccaattattc caaaagaagt aactggagat attttattta
     1201 ttattattgg gagcattgat gcaaacaaga atccccaata tatgtattta tttttagttt
     1261 ttcttcttac atattcatat cctttaacta ttattacttc aagaataata aatattacca
     1321 aatactttag ctggacatca attcctacta ttaagaatat cataaacaaa cttgccaaca
     1381 taccataata ctttatattc ttgcccatta gacctaaaat tactgctggt attgatgtta
     1441 ataataatat ataaagataa aaataatctc catactgtga aaaaatcatt aaatttcctc
     1501 cataagtttc tttctgtcta ttttaccatt gatatttgtt ggaaactcag agattattga
     1561 aatatttctt ggaatcatat atgaaggtat atactttcca agttcctttt ttattatcat
     1621 cccattttta atattactta aatcattctt ttcatttaac tcaacaatac cttttaagta
     1681 agcaattttt tcgtccttat atactggtaa aactactgca ttttttacat tatgtacttt
     1741 tctcaaatta ttttctatat cttctatttc aattctaaat ccatttaatt taatctgaaa
     1801 atcttttctt ccacaataat atatatttcc atctaaaaga tatcccatat ctccagtttt
     1861 ataagctctc cactttacac catctatttc atcatagaaa aatacttcat cagtcttttc
     1921 tttattattg aagtaacctt tagaaactga tggtccaatt attataattt cacccttttc
     1981 attttcttta agttcatttc catcttcatc aagtattttt attttacaat ttgacatagg
     2041 gtatccaact ggaagacttt tttcatcatc tatagctttt tgagtcatat catttacact
     2101 tacaccaaca gtagcctcgg ttggtccata tccattgata attcttgtgt ttggaaatct
     2161 actcataagt tctttagtaa gattttttga aagtgcttct cctatgaata tcattgactc
     2221 aaggttagga agcattttac tattaaactc tttttcagtt atacatactc ctgcaaaaga
     2281 aggagttgac acccatacag ctatatctga gatactaaat tgtctaaata gttctttata
     2341 gtctgctaat acatcttttg atattgaaaa taatgtagct ccatggataa gaccagggta
     2401 tattgttgta actgaaaggt caaaagaata tgctggttgg ttcattatta ctttttcact
     2461 tccatctatg tttagatatg ggcttatcca atctgaaaag ctatctaaat tgtttgaact
     2521 tatttgaacc ccttttggct ttcctgtact tcctgaagta aatagtatat atgcattttc
     2581 atcatctttt acccagtttt ctttatctaa tgattttccc tgatattcat ttattatatc
     2641 atttaattta tccatatcaa ttacattgat atctccaaaa tttctttcat cactaaaatt
     2701 aaatattact tttggtttta tctctttagt gacttcaaaa actctatcaa ttggaaaact
     2761 aatatcaaga ggcacatacg ctctacctga ttttaaagct cctatcatac atgccattat
     2821 catgttttct ttatttccat aaattactat aggagtatcc tcttctttgt atacatcctt
     2881 tagaaatact gatatagcat ctgaatattc atttaaatct ttataagaaa gtttatctcc
     2941 attacacatt agtgctgttc tatctgtatt tgaatatttt tttattcctt caataatttt
     3001 catataagaa tgctccttta aaatccgtta taaataaatg gcaattcttt aaatggagta
     3061 aatgttagaa ctcctatcgc caatattata accatagtta aacaaatact agttaacaaa
     3121 tctttattgt tctttaaata ttttaaataa tctctcacag acatctaccc atcctttcgt
     3181 accaagatgc attacatccc ttaaataata cttatctgac cctttatctt taaggtttag
     3241 aacttcaaag cctttgcttt ctgctatttt ttgtgcttta tcgtagtact gatttctttc
     3301 tttttcagaa ataccagtca aattatagaa tttatccata cttgggatta atacaacaac
     3361 tggattaata cctaagtcag tacatacatt tagcatcagt ttataatttt caaactcctt
     3421 agatgttaat aaatttacat ctttgtctct tcctttgtat tgctctatac catctttaaa
     3481 atgctcttta taatagtact tatcaatatt tagtggattt ttaccaactc tttttttagc
     3541 atcttcaatg gctttctttc tctcatatga ccaatttatt ggtctttttc tttttgttgc
     3601 tctcttttta tctagcttaa ttaatcgttt ataaagaata cccttttctt taagagctat
     3661 acagtacttt cttccttgaa aataaggctc taaaagcacc ttttcagctt tttcaggaaa
     3721 tgtcttaggt tcatatagct tagcatataa tgcttctgct ttatactcat cacttcccca
     3781 taatagctta cttgattttt ttgcatattc aatcttattt tgtttagata tttttggatt
     3841 atctaaaaat cgataaaatt gtattggtga aaatctagac tgataatggt gacttgtgac
     3901 tccgtctttt tccataaacc attgcataga aattaacaag acaactttct tattatcaat
     3961 attaggattc atacttccta atattgcagc atcttgtaat gtctgagtat aggctctccc
     4021 aattgcaaac accttatttt tactcctatt tgtattaaaa tagtactttg gatgttgttt
     4081 tgttgaatta ctaagttctg atgaccccaa aatcataata tccttttctc ttaaaaaatg
     4141 attatttgct gtaacccctt tatcttttat atcacttaaa gtatcatcca taattggaag
     4201 tagatttttc tccctcaaaa gttcatcagt tttagaatca agaaatttgt ctaacccaaa
     4261 taaaaatact acgcctatta taaaaggtgt aataaagtat atcaattttc tcatattttc
     4321 tctcctctaa aaatattcaa atttatgtta ttattaatca tattagtttt gatatgtata
     4381 ttcaactaca aaattgtaat aaaatcgtca aaactttttt tgatactgtt acaaaattgt
     4441 aaaaaaaaag aaaaaaacac caaaaataaa tttcttttta gcactttttc atacttataa
     4501 tttgacattt taacacttgg ctaagtatac accttacctt aacttaaaat taaatttatt
     4561 taagaatata tattataaat tcttatattt tctaatataa gctatatact atcagctgga
     4621 taaacaatac tactttgtct tcttatctca tccataattt ccattactat tattgaattt
     4681 tcatgagaat ttatatttga ttctatttta ttgttattta taagacttat aaactctaca
     4741 agttcataat acattccttc tatttctcta ggcttttcct ctgctttttc ttttggaata
     4801 gatatatctt cttctcttcc atctctgtat ttaataatag ctttttcaaa tagattaagc
     4861 ttttcaatta ttatacttcc ctcttctcct tgtatttcag atggtatata agaatcagct
     4921 actttagaat actgaattat tgcatccata tcatcatatt gaaatacagc tgaaccttct
     4981 ccatccactc cagtttgaag tatatgagaa actgccttta cagtttttgg agcaccaaat
     5041 aaattcacca taggatgtat acaatacact cctatatcca ttaaagctcc atttgacaac
     5101 tcttttttaa aggcattttc tataattcca tttttatatt tatcatatct tgaagaatac
     5161 tgacaatagc ttccaaaaaa tcttcttatt ttccctattt tatacaagtt ttctttaact
     5221 gctttaaagt ttggaacaca tgtaattctc atagcttcca taagcaatac attattatct
     5281 ttggctgttt ttatcattga tttgacttct tttaaattag atgctaacga cttttcacaa
     5341 agcacatgtt ttttgtgttt taaacaagta atggcttgct ttgaatgtaa tgcatttgga
     5401 gaagctatat atacagcatc tatatccttc gatttagcca tttgttctaa atcatcaaaa
     5461 aaaatacttg ctccatgtag agttccaaat tctctagctt tttctaaatt tctagaatat
     5521 acagcaacca attcaaaatc tttaacccta gatgcagctc ttagaaatat atttgctata
     5581 ttgcttgtcc caacaatacc aaatcttatt ttacccatca taaatcactc cccattttct
     5641 acactttatt ttaattttaa taaaaaactt tacaacttat agatatatta taaaataatt
     5701 ttatcaaata aaaaagaaag ctaattttaa atgatttcac cattttaaca tagctttctt
     5761 ttaactacaa tctaaattca acttatattt ttaaagttat ctttctacta tatctttatt
     5821 tcccctcaaa aattttatag taagaatttc atccacaaac aatgttacta aaagtatagt
     5881 tacaagaatt ggccaataag gtattataag actatataaa ctatcaacta tatacacttg
     5941 aaaatatcta actccaataa atgtcaatat agtccaatat acagaaaact ttagacatac
     6001 tattcctcta ctattgtact tataatctga ataatcccaa tagaccttat taaaatatac
     6061 tttcataagt attcctgtaa tatattctac tactgtcggc actactagac ataataaaag
     6121 caatgcaaat atattattgg ggtcaataaa tcttgagacc tctattataa tcgacattgc
     6181 aattgcatac attggtttaa atggaccatt taaaaatcca tcttcttgaa aatgcccttg
     6241 tttataataa ctatataagt tttcaattat ccaacctata aaaccataca agaaaaaatt
     6301 aaataaaaaa tataatagag aacctacatt atccatgaat attcctccta gctgtttata
     6361 cttatttttt ctcatattca tggatttatt ctaaatcaaa gaaattatat ttaatttatt
     6421 taattaatac tttatattat agattgtgac attctttaga gttcaaactt tttaggtatt
     6481 ctctaacttc cagtaaattt tcaagtatct tatgtgattg tgatttaatt tgctcatctg
     6541 gctcttttaa atctggtaca tttatacatc ttatcccacc attgtatgca gcttcaactc
     6601 ccataggcga atcctctatg acaatacaat tttggggatt aacatttatc tttttagctg
     6661 cttttaagaa tatctcaggg tttggtttag agtttacaac atcatctcca caaactatag
     6721 catcaaagta atcttttaaa tttgccttag ctaatctctt tactgctctt tctctctttg
     6781 ttgatgttgc aacagccatt ttgtatccat tttcctttaa aaaagagatt agttcattga
     6841 ctcctaattt gataggagct cccttcctct ccataaattc aatcatattt ttagtttttt
     6901 cgtcatataa atctattatt ggtacagaac tatcgtatat atctgtaaga ccttctatta
     6961 ttcctttacg atttcttccc ataactgatg tataaatttc cttagtcata gtatatccat
     7021 acttttcaaa cgtctccatc caaaattcaa gagaaattct ctctgagtca aacaaaactc
     7081 catccatgtc aaaaataatt ccttctactt tttgcataca attcctccaa tattttttga
     7141 tataattata ttacactttt tatgattaat tttacaaccc tcaatttatc caattctaat
     7201 attgtaaaat tgtaattttc aaaagttacg ctttggttta ctttaatatt tgaaccaagc
     7261 atataggata tccacccacc tatactgtca aatccatcat gctctatatc aagtccaaaa
     7321 tacttattaa tatagttgat agattcagtt ccatcaacta agtaagaacc tgctcctatt
     7381 ttcttaatat gagtctcttc tttgtcaaat tcatcttgaa tttctccaac tatctcttct
     7441 agtatatctt ctattgttac cacaccagat gtaccaccat attcatccac aactatagca
     7501 agttgaagtt tttcctttct tattctctca agagctttat ctatagttaa attttcagat
     7561 atatatataa tatctctcaa tatttcttct agttcaattt tattttcatt tatcttttga
     7621 ttgtataaat ctcttatatg tacaaaacct aatatatcat ctttattttt tctgcatact
     7681 ggatatcttg taacaccttc ctcttttaaa atagctaata tcttttcttc agaatcactt
     7741 tcatatatac ataccatatc agttctagga accattatct ctctaatttt cttttcttca
     7801 aattcaaaaa tattatcaac taatctttgc tctgattcat ctataagtcc acttttataa
     7861 ctttcttcta ctaatagtct tatttcgtca cctgtatggg gttcatcaac ctcatcagct
     7921 tgtgaatatc caaatggttt aagaaataaa tcagtactta agttgaaaaa atatattatt
     7981 ggatatgtta acttataaaa tcctactaat aaaaaagatg tactcaacat tattttttct
     8041 gtattataaa gtgctaaagc tttaggaacc aactctccta ttacaacctc catcattgtt
     8101 ataattaaaa aagaaatgga aattgatata gtatatataa tactttcact aagattaaat
     8161 acacttacta ttggtaaaat taactcttta actgtagatt ctcccatcca acctaatgca
     8221 agtgaacaaa gtgttatacc aagttgacat gcagaaagat aggaatttaa gttgtctttt
     8281 attattttac atctttttgc attattatta ccttcagtta tcatggtatc aatcctagaa
     8341 ctacgaagtt ttaccatagc aaattcacaa gctacaaaaa aagcatttat caaaacaagc
     8401 attactataa gtgttacttt taataactcc cgcataaagt attggttctt ataatgtaag
     8461 aaatctataa gaactcaaag ttcccccctt atatttataa atttaattta taatatatat
     8521 tctatcatat ttattaatat atcctacttt attacaaata attttta
//
LOCUS       DAELSY010000157         8492 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.157, whole genome shotgun sequence.
ACCESSION   DAELSY010000157 DAELSY010000000
VERSION     DAELSY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8492
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.157"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..749
                     /locus_tag="KPG09_003215"
     CDS             <1..749
                     /locus_tag="KPG09_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG0967795.1"
                     /translation="NQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFNIIDTFNSYVK
                     PTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCVWGVGDLKELY
                     RNINYHKLPSSSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELDEKMSYHNALN
                     DAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDEFRKILNRELT
                     KDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(841..1593)
                     /gene="xth"
                     /locus_tag="KPG09_003216"
     CDS             complement(841..1593)
                     /gene="xth"
                     /locus_tag="KPG09_003216"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBG0967796.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(1754..2704)
                     /gene="galT"
                     /locus_tag="KPG09_003217"
     CDS             complement(1754..2704)
                     /gene="galT"
                     /locus_tag="KPG09_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBG0967797.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            3089..4285
                     /locus_tag="KPG09_003218"
     CDS             3089..4285
                     /locus_tag="KPG09_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0967798.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(4397..5743)
                     /locus_tag="KPG09_003219"
     CDS             complement(4397..5743)
                     /locus_tag="KPG09_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG0967799.1"
                     /translation="MTMKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVV
                     EERQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKI
                     PLQFGLVGQETCGAGGFAYGLRSIGDMIEMVKTVRKYSPKAWILNYTNPAAIVAEALK
                     RVFPDDKRLLNMCDQPVNLLRSYGRLLDMDSRTFEPVYFGLNHFGWFTHLYDKNGEDL
                     VPKIKDLVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKV
                     AKSNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAH
                     NKHQIAIVITENNGAINNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAY
                     EKLAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIKVNKDYWPTLYKENEK"
     gene            complement(5876..6628)
                     /locus_tag="KPG09_003220"
     CDS             complement(5876..6628)
                     /locus_tag="KPG09_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0967800.1"
                     /translation="MNLIDKLDINQKKLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
     gene            complement(6787..8364)
                     /locus_tag="KPG09_003221"
     CDS             complement(6787..8364)
                     /locus_tag="KPG09_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0967801.1"
                     /translation="MKQKMKEFFLQLGKSFLLPIALISAAGIFLGISSAFSNPNIVSD
                     IPFLENNFIQGILGFTKAITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVL
                     HTSVNYMLGCRGILAEPDMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNI
                     KLPDYLGFFGGTRFVPIITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFV
                     FGFLERLLIPFGLHHILNAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPETISFST
                     EATRFLAQGKIPIMVFGLPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLE
                     FSFLFVSPILFVIHAFLSGISFMMMHIFGVAIGNTQGGIIDLVVFGVMQPNTKWIYSI
                     ILGLVYALVYYNVFKVLILRFNLQTPGREEITDINFDLEPLSNKVEIAQSGETTKRAL
                     SIIKYLGGKENIEDITNCITRLRVVVKDMSKVDENAFKKYTGAMGVIKVSDKDIQIIY
                     GPSVSQVRDEVSFAMSI"
BASE COUNT         2953 a         1383 c         1013 g         3143 t
ORIGIN      
        1 ttaaccaagg atttgataaa aaactcaata aaactgtatc caatgaaaag tgtccttttg
       61 aaattataca aataggagca attaaattgg attctaaatt caatataata gatacgttta
      121 atagctatgt aaaacctaca atttataaag acatacatcc ctttattagt cgtatgacta
      181 atattaaaaa ttctgatttt aatgattcgc ctactttccc tgaggtctac aacaatttta
      241 taaaatttat atcatcacaa gaccctatat tatgtgtttg gggagttgga gatttaaagg
      301 aattatacag gaatatcaac tatcacaagc taccatcaag ttccttacct aaatcttata
      361 ttaatataca acaacatgca tctaaatatt tcaataatcc agcaggaaaa agcattggcc
      421 tacaaaatgc tatttctata ttggaacttg atgaaaaaat gtcttatcac aatgctttaa
      481 atgatgcata ttatactgct aaagttttta ttaagatata caatccaagt atagtcccag
      541 atatatatct ttatacgagt ataaaaccta aaactataag atattctaat aaaaaaagag
      601 ttgattatga taagttattt gatgaattta ggaaaatttt aaatcgtgaa ttaactaaag
      661 acgagaaaaa aatcattaac ttagcttata atatggggaa aacaaatcaa tttacattag
      721 aaaatgttaa gcaaagaaaa aacaaataga gtttatttag ataaaaaata ggcatgtaga
      781 ttaaaaaact agttaacttt aaatttatat ctacagccca atttttatcg taattaattt
      841 ttattttatc tctaattcaa ctggacaatg gtctgaacct aatatttcag tgtgtatatc
      901 cgcacttact aatctatctt ctaatttttt ggatgcacaa aaataatcta ttctccaccc
      961 tgcattattt tttcttgcat taaatctata tgaccaccaa ctataaacgc cttctttatc
     1021 aggattaaaa tatctatatg tgtctataaa cccactgtct aataatttag aaaacttctc
     1081 tctttcttct ttagtaaaac ctgcattttt catattattt ttaggatttt ttaaatctat
     1141 ttctttatgt gcaacgttca tatctccaca cacaattaca ggcttatgat tatctagttt
     1201 ggttaaataa tctataaagt cgtcttccca tctagttcta tattctagtc tctttaattc
     1261 agattgagaa tttggcgtat atactgtaac aaagtaaaaa tcttcaaact ctagagttat
     1321 cactctaccc tctttgtcat gttcttctat atttattcca tacattactt ttaaaggctc
     1381 tttttttgta aatatagcag tacctgaata accctttctc tcagcataat tccaatattg
     1441 aaaatatcct ggtaaatcta actctatttg cccttcttgc aatttagttt cttgtaaaca
     1501 aaatatatct gcatctactt ctttgaaaaa gtctaaaaat cctttcccaa cacaagctct
     1561 tattccatta acattccatg atataaattt catacttata ctcctcatct aaacattgac
     1621 acactcccca tagctaaaga tgggggcttt tcaacaattt tattgtaaat tcttaattta
     1681 cttttatttt aacaatacta tactataatt tcatctataa ttactattta cttaatacct
     1741 aaaaatatat gttttaatca atttttaact tttttgttaa atcttcaggg ctcattgaag
     1801 aaacatacat atttgttcca agttcaaatc ctccaaaatt atattttctt gggattatgt
     1861 gcatatgaac attaaaatac gattttgttt caatctttgt tggatgagtg tgaatacata
     1921 agttaaatgg catatatcca ttgatattgt ataacttctt aaacaacttt tcaaatattg
     1981 tagaaagctc ctgtatatta ttatcattta actcaccaaa ctctatttta tctttaaata
     2041 ttattcttac ttctccacta tacttggttg catatggtat aagtaccaaa aaagcttctc
     2101 cattataaac aactcttcta tctaaactta tttcattttc tattaaatca tcatacaaag
     2161 agcttttatt gctgtcataa tactcctttg aaactaacag ttcattcgta atttcaggtg
     2221 gaataaaaga catagacaat atttgagcat gtggatgcat caaagatgct cctgcctttc
     2281 tcaagaagtt tttgaagata ctaacatact ctactttatc atctttcgac aaatctctaa
     2341 atctatttct atacataaaa aaaacatctt caaattcttt ttgactcatg ttataaaaac
     2401 tacgattatg tttatcgctc tctatcatta cttcatgaac accatataca tctcttgcca
     2461 tatcatcaat tataggaaat ttattgtaga cagattttac aagccatcct tcttcacttt
     2521 ctatttcaaa taatgcatca gttgcatgag cttcatttcc tttgcaaaat gggcattctt
     2581 catcatattc attagtttct tcatcttcat ttgggatatc tgctgtatct aaaggtcttt
     2641 ttaacctatc tgtagcaaat ataacaacat cattagtaat tggatctatt cttaattctt
     2701 tcataattaa cacctcttag ttaatagcct ttaatataat tatatgatat ttatcgtatc
     2761 tgttaaataa atttataata ttttttataa ttttgcatat ttattattat atattttgtc
     2821 tatagctgaa agattataac taaaaaatca ctatcagtgt atcagaatat tatcttacta
     2881 taaattaatt tatgaataca taatttagtg gcataaacat gggtatttta ctataatttg
     2941 ttaaaagttt attttaaagc tatattaatt ttttttgatg atttttaccc ataagtatgt
     3001 tattattatc ttgtaaagat gtcttgtatt caaacataca atttacataa tacaatatac
     3061 gtatttaaat taaatagggg gtattaaaat gaattattca aacagagtta gtgcaatgca
     3121 agcctctcca atcagaaaac ttgttccatt tgcacaagca gcaaaagata aaggaatcaa
     3181 agtatatcac ttaaacatag gacaaccaga cataaaaact cctaaaggat tttttgatgc
     3241 tgttaaaaac tttgatagcg aagtcttaga atatgctaca tcagaaggta ttccagaatt
     3301 attagaagca cttcaaaatt actataaaac ttacgatatg aattttgaaa aagatgaatt
     3361 attagtaact aatggtggta gtgaagcttt acttttcact atgatggcag tttgtgaccc
     3421 aggagataat ctattagttc cagaaccatt ctatacaaac tacaatggat ttggtcaatc
     3481 tgtaaatgta gaagttaatg ctgttacaac aaaggcagaa aacggtttcc accttccatc
     3541 taaagaagaa atattgtcta aagtagatga taaaactaag gctataattc tttctaaccc
     3601 aggaaatcca actggtgcaa tttatacaaa agaagaactt aatatcctag ctgaaatagc
     3661 taaagaaaaa gatttatgga taatagcaga cgaagtttat agagaatttg tttatgatgg
     3721 gcttgagtat acaagctgtg gtaacttaga gggtgtacag gatagagtta taataataga
     3781 cagtgtttct aagagataca gtgcttgtgg tgctagaatt ggttctatcg cttgcaaaaa
     3841 taaaggtctt atcgctcaaa tacttaaatt atgtcaagga agactttgtg ttcctacatt
     3901 agagcaaata ggagctgttg agttatacaa aactcctgtt tcttacttca aagaagttaa
     3961 tgaagagtac aaaaaaagaa gagatgtatt atacaatgaa ttaatgaagg ttgaaggtgt
     4021 tatatgtaaa aaaccaactg gtgctttcta tatagttgct aaattaccag ttgaaaatgc
     4081 agaagatttc acaatttgga tgttaaaaga atttaacaaa gataatgaaa cagttatggt
     4141 ttgccctgct gaaggattct atgctactcc aggtttagga cgtgatgaaa taagattagc
     4201 ttacatctta aatgaaaaag accttcatag agctgctaca ttattaaaag aaggtttaga
     4261 gcaatatgta gctttacaaa aataattttt taaacttaat ttatataaat ttaatgatat
     4321 aaaatgtccc cttttcagat attatttgaa aaggggacat ttcttaatta atatattaat
     4381 taagacttag acctgactat ttttcatttt ctttatatag tgttggccag taatctttat
     4441 tcactttaat taaagcatct aaaacttgtc ttgccttagt tggagagatt atagttctat
     4501 ttaatgttaa agataaaata gctttttcat aagattgttc aaagtatgca tcaactgcta
     4561 acttttcata agctagttga ccttctaaca gtcccttata aaatgttggt atattaccta
     4621 catgtaaagg tctcggtcca ttctttgtaa gaatcgcagc tacttctacc attgcatcat
     4681 caggtaagtt gttaatagcc ccattatttt ctgttataac tatggctatt tggtgtttgt
     4741 tatgagctat tgcttctgca acttcaacta taaattcccc atgagcatca ttatgcacta
     4801 aattagaacc tatagatgtt cctttttcta taacagattt acattcttca aatacacgct
     4861 tttctcttcc attcataact tctcttgctc tagtacaatt tatatttgat ttagctactt
     4921 ttttatcagg atatagataa tactgtacat atgtattagg taagtaatct ggtacatctt
     4981 ccaacatatc tttaaccatt ccataagtgt ctaaccaaga tttatctctt tgttctgcat
     5041 caacaggttg gaatccagag tttgctacta aatcttttat cttaggtacc aaatcttcac
     5101 catttttatc atacaaatgt gtaaaccatc caaagtgatt taatccaaag tatacaggct
     5161 caaatgttct tgaatccatg tctaacaatc ttccataaga ccttaacaag ttaactggtt
     5221 ggtcacacat attaagtaat cttttatcat ctggaaatac tctttttaat gcttctgcaa
     5281 caatagcagc aggatttgta taatttaata tccatgcttt aggagagtat tttcttacag
     5341 tcttaaccat ttctatcata tcacctatag accttaaccc ataagcaaat ccaccagctc
     5401 cacaagtttc ttgaccaacc agtccaaatt gtagaggaat cttttcatct aattctcgca
     5461 tcttaagacc accagttctc atttgaacaa atacaaaatc tacatctata aatgcttcat
     5521 ctgggtttgt agtatattca agtgttgctt taggatattc ttctttaaat aatatttttc
     5581 caaattcccc tattaatctt tgacgttctt ctactacatc taacatagtt actttattta
     5641 gaggaaatct atcactcatt ttacataaag actttaaaag tcctggagtc caagtactgc
     5701 ctcctccaac tatagctata ttgtatcttt ttttcattgt catattaatc acccctgttt
     5761 tttattaact aacttgtaag atgattataa tatataaagt tttctacatg aatactttta
     5821 ggtaatcatt aaaaatagtt caattttagt atttttttca aaattgtaat ttttttcatc
     5881 tattgtattt ttcaatgtat ttatgaaaca tatattccat aagagaaagt gctggcactc
     5941 tagacgtata gttaacacta ttaaaattat attgtggaga agatacaaat aattttatat
     6001 cagctatatt tgataatgaa ttgacactta tatttgtcag agaaattact ttaacttgat
     6061 ttgtttttgc tatattacat gccttaataa cttgagcagt ctctccagac atagatacag
     6121 caattacaag gtcatcttta gtaatatttg atgcagccaa atacatgaga gttgaatcat
     6181 catagtattt tacatttaca cctatagctg aaaaacgttg agacatttca gtacatacta
     6241 atctagaaga cccaacacca aaaaaatata tatgcttaga attatgtatt tcatttaaac
     6301 acatatctat attctctaca tttattaaat tttgtgtatc cataacatca gtaaaaatat
     6361 cactttgact tttagtaaac tccttatttc tttcatttct atttgaatat gaaaaaacta
     6421 aaaagttttt aaattcacta tatccagaaa agccgagctt ctgacaaaac cttactatgt
     6481 atgcagtcga attaaaagtt tcatttgcta tatctttaat ctttttttga tttatttttt
     6541 cagaattttt tagaaaatag tttaaaagag atatttcatt tgtagataat tttttttgat
     6601 ttatatctaa tttgtctatt aggttcattt atacctccat aataatgatt tgtagttaaa
     6661 caactttctc atatttatta tataaatata cactatacaa catttatctt atgtttatta
     6721 tactacaaaa aagacaatac aaattataaa ttagtattgc cttttaatat ttacattaga
     6781 tagacattaa atagacattg caaaagaaac ttcgtctctt acttgagata cagatggccc
     6841 ataaattatt tggatatctt tatcagatac ttttataacg cccattgcac ctgtatattt
     6901 tttaaaagca ttttcatcta ctttactcat atctttaact actactctta agcgagttat
     6961 acagtttgtt atatcttcta tattctcctt accacctaaa tatttaatta tggataatgc
     7021 tctcttagta gtttctccac tttgagctat ttctacttta tttgataaag gttctaaatc
     7081 aaaatttata tctgttattt cttctcgtcc tggagtttgt aagttaaatc taagtatcaa
     7141 aaccttaaat acattataat aaaccagtgc atacactaaa ccaagtatta tagaataaat
     7201 ccactttgta tttggttgca taactccaaa tactactaaa tctattatgc caccttgagt
     7261 atttcctatt gctactccaa atatatgcat catcataaat gatatacctg ataaaaatgc
     7321 atgtataaca aataatattg gtgatacaaa taagaatgaa aattccaatg gttccgtaat
     7381 tcctgttaca aatgttgcta aagcaccagc tagtaatata cctttaacct tcgacctatt
     7441 ttcaggcttt gcagttttat acatggcata tgctgcagca ggaagaccaa ataccattat
     7501 aggtattttc ccttgagcta aaaatctagt tgcttctgtt gaaaaactga ttgtttcagg
     7561 gtcacttagt tgagctaaaa atatgttcaa tgctccaaat acttcttgtc ctcctacaac
     7621 taaagaacct cctacttcag taaatctaaa catagcattt aatatatgat gtaatccaaa
     7681 tggtattaaa agcctttcaa gaaatccaaa tacaaaggtt cctaaatatc ctaaactagc
     7741 tataccaata cctacactct gaataattgt atttacatat ggccatatat atggtattat
     7801 tatccccaaa atagagaata ctactgttgt tattattgga acaaacctag ttccaccaaa
     7861 gaaccctaaa taatctggca gttttatatt atagtattta ttatgtaatg atgctgttat
     7921 tataccaact acaattccac caaatactcc taactctaat gtttgtatgc ctaaagttac
     7981 agcttgaccc atttctctca tcatgtcagg ttcagcaaga atacctctac accctaacat
     8041 ataatttaca ctagtgtgta atactaaata accaacaaaa ccagaaaatg cagaaactgc
     8101 tttttcttct ttagcaagac caacagatat tgatatagca aagaatattg ctaagtttcc
     8161 aaaaagtact cctgttatag cttttgtaaa acccaatatt ccttgaataa aattattttc
     8221 taaaaaaggt atatcactaa ctatattagg atttgaaaat gctgaactta ttcctaaaaa
     8281 tatacctgca gcagatatta atgctattgg caataaaaat gattttccta actgtaaaaa
     8341 aaactctttc attttttgtt tcatttctat ccctccattt ttaaattttt aaaacgtttt
     8401 ctatgtcata attatatatt tttattttgt ttttgaaact ttataatttg ttttaaaata
     8461 tttttacata aatagtaatt ttttcaaaac tg
//
LOCUS       DAELSY010000158         8402 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.158, whole genome shotgun sequence.
ACCESSION   DAELSY010000158 DAELSY010000000
VERSION     DAELSY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.158"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..240
                     /locus_tag="KPG09_003222"
     CDS             <1..240
                     /locus_tag="KPG09_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0967802.1"
                     /translation="FIRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLNI
                     REWICPSCNEIHDRDINASINILKEGLRLITIQNK"
     gene            305..766
                     /locus_tag="KPG09_003223"
     CDS             305..766
                     /locus_tag="KPG09_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0967803.1"
                     /translation="MQNSVKQEPCDFSHGRFRFGGKIIRRNEKTKFDWLSRDKEQVEK
                     YINDPFCGVVCSCGFFYDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGV
                     LRLRDRYIKLGVKDVTCKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            1231..2445
                     /locus_tag="KPG09_003224"
     CDS             1231..2445
                     /locus_tag="KPG09_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0967804.1"
                     /translation="MQIKIGKVIQRLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEKVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNIFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLWYANLSLEIKKMLSEDKGAFFSMYVDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(2865..3386)
                     /locus_tag="KPG09_003225"
     CDS             complement(2865..3386)
                     /locus_tag="KPG09_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0967805.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHEQRKSYLQTEILKNKE
                     YQFNLIYDKNQFVGIILCWETDNFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            3742..4539
                     /locus_tag="KPG09_003226"
     CDS             3742..4539
                     /locus_tag="KPG09_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG0967806.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            4649..5239
                     /locus_tag="KPG09_003227"
     CDS             4649..5239
                     /locus_tag="KPG09_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBG0967807.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNEETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFADFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            5305..7002
                     /locus_tag="KPG09_003228"
     CDS             5305..7002
                     /locus_tag="KPG09_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG0967808.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGISLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     NVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            7216..8055
                     /locus_tag="KPG09_003229"
     CDS             7216..8055
                     /locus_tag="KPG09_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBG0967809.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
BASE COUNT         3352 a          809 c         1413 g         2828 t
ORIGIN      
        1 tttattcgtc aattagaata caaggctaat tggcatggta gacaaattgt aaaggtaggt
       61 aaattctttg caagttcaca aatatgcaat aaatgtgggt ataaaaacga ggaagtgaag
      121 gacttaaata taagagaatg gatttgtcct agttgtaatg aaattcacga tagagatata
      181 aatgcaagta taaatatatt aaaagaggga ttaagactaa taacaattca aaataaataa
      241 cttatgtaag aaccgtagga actacgggga tagcctgtgg agaattagta agacatattt
      301 tagtatgcaa aattctgtga agcaggaacc ctgtgacttt agtcatggga ggttcagatt
      361 tggtggcaaa ataataagaa gaaatgaaaa gactaagttt gactggttaa gtagagataa
      421 agaacaagtt gaaaaatata taaatgaccc attttgtgga gtagtgtgta gttgtggctt
      481 tttttatgat ttagttcaag ggttaaaaga aattgaggat aaagaaaact taaaaaaagt
      541 gccacttgat atacctattt acataatatc tggagataaa gaccccatag gaaaaaatgg
      601 taaaggagtc ttaagactaa gggatagata tataaaatta ggagtaaaag atgtaacttg
      661 caaactttac aaagatggaa ggcatgaatt gttgaatgaa ataaatagag aagaagtttt
      721 tgaggatata atttgttggt taaacaataa aatagaaata ttgtaataaa aaaatataat
      781 gatatatttg ctttaaattt atttatatat aatatttgaa aagtggatta tgtatatgca
      841 aagtagaaaa gagagagcct agaagtgatt ttttgctata atgccattta ttgaataggt
      901 gggtgttatt ataaataaat tattaacact cagtattatt atcattgtat atggaattta
      961 ttttatttaa aattccttga aagtttaaat atagtaagtt gtagtaaaga gaaaatctaa
     1021 atatttaaat gctatttaat gtgaatctat taattaggtt atacttaaac ttaattaata
     1081 gattttatat ttcaagccaa tgagataggg ttttagatta aaatataacc aatggttaaa
     1141 ttacagacat tttgttaact ttccctcgat ttactagtaa atattttttt tatatactta
     1201 aattacaaca gatgtactag ggaggtatat atgcaaatta aaataggtaa agtcatacag
     1261 cgtcttagaa aagaacgaaa tttaactcaa gaacaattag caaagtttat aggtgtgtca
     1321 acaccagcag tatctaagtg ggagagtgga aattcttacc cagatataga attattgcct
     1381 ttattagcag acttttttaa tgtgtctatt gataagcttt taaattacaa gattgattta
     1441 agtgaagaaa aagtaatgaa aatttataaa gaattagaat caggctttgc tagaattgaa
     1501 attgatttat caacagaaga accaaaagaa gaatttagac aagacttaga atctgtaaaa
     1561 aagctttcta atatgtatat agagaagtat cctaaaagct atttattgaa gctaaggata
     1621 tgttcgctgt atcagatgta ttcatataaa tttggtaaga gtgaacttaa tgacagagta
     1681 aaagaaacaa cgaatattct tgaagatata gtgagaaata cagatgatat ccaaataaaa
     1741 gaaacagctc ttattatatt atcaaacgca tattgtatgt tggaagatta tgaaaaggca
     1801 gagttgtatt taaatatgat acataagtca ataggggata caagtgttaa tttagctatg
     1861 atatatttaa agcaaaatag acttgaagaa gcagaaatat tgcttcaaaa taaattgttt
     1921 agtaatatat ttaatataag catggattgt aagggaataa ttaatgtata caaaaatcaa
     1981 tacaaggaat taaagaaaaa attggagaat agaaatttta ataaaaatgc aattgagaga
     2041 gaaatggagt atataaaaaa taagttgctg tggtatgcaa atctttcttt agaaataaaa
     2101 aaaatgctca gtgaagataa aggtgcattt tttagtatgt atgtggatta tatggaatta
     2161 tctctaattt ttttattttt taacatgaaa gaagaagcaa aaaaagcctt atatagtcta
     2221 aaagaaatat tagaaaaata tcctatacat gaaagtttgg atgtaagtca aatgagattt
     2281 tttgataagg tagagtctaa aaatttatat acttttaata tatatacaaa cttgttaatt
     2341 gtacttaatg atgacagtta taatgagctt agagaagaac caatttttaa ggatgtaatt
     2401 gaaaagattt tagatatgga aaaaatgttg aaaaataaag aataagtaaa aatttaatat
     2461 aggacttaaa gattaatcaa attaaaagaa agtgtttcaa aataaaaatt tgaatcacct
     2521 tttttgttgg tcttaaaccg ttatttagaa aaatagaggt ttcaaaatag aaatctgaat
     2581 tatctgtatt attacacaaa aatagaaacc taaatttata aaaatagaat aaagtttgtt
     2641 atataaaatg taattttgtc tataaaatct cacatgtttt cttaacttta agcgtgaaaa
     2701 agaggatgta tcatgaattt tttagttcat gatgcatcct tatttgtatg aaatccaata
     2761 tatctttatt atttaaacaa tggaaaagag tcttatttct attttgagac atctctttta
     2821 tttaattatg aagttttata cagttcaata aataggaatt atatttagaa actcatgaca
     2881 ttatttctta gatagtgatt aaatttatca tattccattt tagataactt ttctgggtaa
     2941 gacattacaa tcaggttatg cccattgaat ttttcatggt atggtggatg aatgtgttca
     3001 taattatttg tcttatattt tactctttca tagaacgcct tgcgataaat tgaaatctca
     3061 tcaattggaa ggtcaatttc taaaataata gtttttcctt tcttatttaa aagctctaac
     3121 gctctttgtc catatctatt attgcgcatt tctggaaaaa tgcagaaatg ttctacatag
     3181 ataaagttat ctgtttccca acataaaatt atgccgacaa actggttttt atcataaatt
     3241 aaattgaatt gatattcttt atttttcaaa atttctgttt gcaaataaga ttttctttgt
     3301 tcatgaaatg ggaaactaat tttatataat tccatagcct tgtaatataa tttattttca
     3361 ctggttctta accgttcaaa tttcacttta ctcctcctaa tcacgaaaat tccaatttat
     3421 ttatgagttc attttaccat agtcagtatt tgcatacaat gtgttttaag ttgagtgtca
     3481 tttctttctt ttatttatgc ttatatctca tagactaaga gataattgct taagacataa
     3541 ctaaaaacta ttatattaag ctttgataat ctaaaaacag tatatttatg gacttttcta
     3601 ggtctaaaag taatcttaat aattttagaa gttacaaaaa agttacataa atttggaaag
     3661 ctataatata catatataat ataaggttaa aactgtaatg aataatatta atttaaaatt
     3721 tgaacatgga aggagattat tatggagaaa agagaaaaga ggataataat atcatcttta
     3781 ttaagtgtat caattttaat gggtttggta agtatatatt ctatattaaa taaagaagac
     3841 ataattttaa ctgttaaggg tcaagagcaa aaagtatctt cctttaagaa aacagttgag
     3901 gaacttttgg atgaacaggg tgtaaagtat aattctgaag ataagattaa tccaagttta
     3961 gatacagaac taaaagatga tatgaaaata aaagttgtta aggtaactaa aagtaaaaaa
     4021 gaagagattg aaaaaattcc atttgataca aagcatgtaa atgatagtaa tttgttaaaa
     4081 ggaaaatcta aagtttatca agaaggtcaa gaaggagaaa aaaaactagt ctataattta
     4141 acttaccatg atggaaaatt agtcaaaaaa gtcttatcaa aagaagtaat atctaaggag
     4201 ccaactacaa aaattataaa atatggaact aaagaaaaag tactaatagc atcaagagga
     4261 gcaaatataa gaggaggcaa acatatgaaa gtggttgcca ctgcatatgc aggagataca
     4321 ataacatcta ctggtacaac tccaagatgg ggtgttattg cagttgaccc acgtgtaata
     4381 ccatatggaa caaaagtata tatacctaaa ctgggtatga cttttgtagc agaagattgt
     4441 ggaggtgcaa ttaaaggtaa tagaatagac atttttatga acagtgaagg aaaagcttca
     4501 aattggggaa ggaaaagtat agatatatat ctacattaaa ttagtgaata gaataacttt
     4561 acttgaagga caaaaaagta taaaatatga attaactaaa ctgttagatt attcttaaaa
     4621 cataaaaata tatctaaggg gggataagat gaaaatagtc tgcttaggag acagtttaac
     4681 gtatggattt ggagtatcac gaagtaattc atggacaaac attgtaaatg aagagactcg
     4741 attagaaata gtaaataagg gtataaatgg tgatacaaca agtggtatgt tagctaggtt
     4801 taatgaagat gttgtaaaaa actctcctga tatagttttt ataatgggtg gcactaatga
     4861 ttttattgct ggtgcgggaa atgaaattat taattctaat ataatggcta tggtgcatca
     4921 gtcatattca aaaaatatta tacctataat aggtatacca ttaaaaccag atattccaaa
     4981 tgttagggaa gattggagtt gttttactga ttttaatata gtatctcaaa acttagaatc
     5041 atatagttat tggataaaaa aattttgtat gacatttaat actaatttcg cagattttta
     5101 ttcagaatat aataaaaata tggaactaga aggatataaa aaattatatt ttgatgggtt
     5161 acatccaacc aaagagggtc atagaataat ggcagacatt tttattaatt caattaataa
     5221 gtatatagaa gagttataga ttacattaaa atgcttgttt atgcgtcagt tgcaataaat
     5281 acatacagta gagaggggtt tattatggat tatactaaaa cttatgaaga gtggataaag
     5341 ggttcatatt ttgatgaaga tacaaagcta gagttggaga atataaaaaa taatgaaaaa
     5401 gaaatagagg atagattcta taaggattta gaatttggga ctgcgggtct tagaggaata
     5461 attgaagcag gaacgaatag aataaataag tacacagtta gaagagctac ttttggattg
     5521 gcaaactata tattagaaaa cacaacaaaa gaagaaacaa gtagaggagt agtaatagct
     5581 catgataata gacacaagtc tagacaattc tgtatagaat ctgcaaatac tttggcagca
     5641 tgtggtataa aagcatatat atttgatagt ttgagaacta ctccagaatt atcttttgct
     5701 gttagaagct taaatgcaat cgctggtata gtcataacag caagccataa tcctccagaa
     5761 tataatggat ataaggtata ctgggaagat ggagcacaag ttatgccaga gattgcaaat
     5821 gcaataacag agaaagtaaa tagtatacat gattatagta caataccaac cttaacggaa
     5881 gctaataaaa atttggtagt tttgttggac gaatcacaag atactaaatt tatagaagca
     5941 gtaaaaagcc aaattataag aaaagattta gttaaaaatg taggtaaaag ctttaagata
     6001 gtttatacac ctctttgtgg aacagggaat gttccaataa gaagagcatt gaaagaagtt
     6061 ggttttgaaa atataattgt agttccagaa gaagaaaatc cagacccaaa ttttgcaggg
     6121 cttgattatc caaatccaga agagaaaaaa gctttaaata gaggtatatt actcgcaaaa
     6181 gaaaaaggag cagaccttgt aatagcaact gacccagatt gtgatagagt aggtgtagca
     6241 gtaaaaacta ctacaggtga gtatgcttta cttacaggaa atcaaattgg tggtatgctg
     6301 acacattata taatagaagg attaaaagaa aataacaaat taaaagaaaa tcctacaata
     6361 ataaaaacca tagttacatc agaatttgga gcagatatag ctaaagcaaa taatgttgat
     6421 gtattaaatg ttcttacagg ttttaaattt attggtgaaa aaataaaatt atttgaacaa
     6481 aataaaaata gaagttatgt atttggatat gaagaaagtt atggttactt agttgggact
     6541 catgctagag ataaagatgg tgtagtttct tcattgttaa tatctgaaat ggcagcattt
     6601 tactattcaa aaggtataag tttatatgat ggtttaatag aactttataa aaagtatgga
     6661 tttttcaaag aacagactat atctttgact ttaaaaggta tagaaggtgt agaaaagata
     6721 aaagaaataa tatcttattt tagagaaaat caaattgatt atataaatag tattaaagta
     6781 gttgataaaa aagattataa aaatggtata gataatcttc ctaaatctaa tgttttaaaa
     6841 tactttttgg aagatgaatc ttgggtagct ataagaccat ctggaacaga accaaaactt
     6901 aaattttaca tagctgtaaa aggagctagt gatattgaag cagataaaaa gattcaaggt
     6961 ttgaaaaagt atatagatga catggtcgaa aaattaaaat aaaatattat tattttataa
     7021 gataattttg taataatatt tatttagctg aaaattataa tttactttca ttgagtagaa
     7081 tatggaaatg ttagtagaat tgttattttc agcttttatt tgtgttattt ttacttaaat
     7141 aattatatta tagaatgaat ctgagtataa ttaatattgt ataatgaaat taatttgata
     7201 gaaaagggga tatttatgtt tggtatagat aagaaattga ttgaatatat aattgcaaaa
     7261 tccaattaca gtgaattaag ttcaaagtca gcagaagtat ctttcttctt aatgttgtcc
     7321 atatttcctt ttttaatatt tacaataagt tctatagctt atataccgat tcttcattta
     7381 aataagtata tagctttgtt tagaaatatg atgccagaag gtgcttttgc tgtgttatct
     7441 tcaatcatag tttctgcaat agacaataga aacctgaaat ttttagcagt cagttttgta
     7501 cttactatgt ggacattttc aagagcagta aaagctctta ttaaaggaat gaatagagcc
     7561 tataaagtta aagaaactag gtcttttttt aaaattttat ctatatcatt tttatttaca
     7621 ataatgcttt tggtccttat ttttttatct atgatatttt tagtttacgg ggaaaaaata
     7681 ggttatttta tttttaatct tgtaggattg gatgagatat ttataaaaat atgggatatt
     7741 ctaagataca ctgtaggtat aataacaata atagtgatat ttacactttt atataaatac
     7801 actccaaata aaaagttaac tattaaagaa tctgcgccag gtgctatttt tgctactttt
     7861 gcttggtttt tagtatcatt tttatattct tattatacaa attactatgc taactatgaa
     7921 gtaatctatg gaagtatagc tgaaataatt gtacttatga cctggatgta ttttagtagt
     7981 tggtctatcg ttattggcta tgaggtaaat tcaagattgt attttagaaa gataagacat
     8041 gaaatgttaa agtaaagtat ttaaaataaa agagagtatt tagtattata aatgtaattg
     8101 aattaataaa gaaatattga catttaagtt aaaaagtcta gaatttttct gggctttttt
     8161 tatttttagt ataacttgac aaattttatt taatgggata gttttaattt ataatcatct
     8221 ttgaaaagat gactattgat aagtaaggta taaaattggt aagtatcaaa aaatatacta
     8281 aataaaaatt atagtcctaa tgagttaatt tctgtatttt caaataatgc agtatttagt
     8341 catgtgtttt tatatgcgcc tgtatttcca attaccaata tattagaata agctactgta
     8401 at
//
LOCUS       DAELSY010000159         8371 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.159, whole genome shotgun sequence.
ACCESSION   DAELSY010000159 DAELSY010000000
VERSION     DAELSY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8371
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.159"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            306..1682
                     /locus_tag="KPG09_003230"
     CDS             306..1682
                     /locus_tag="KPG09_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBG0967810.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1993..2664
                     /locus_tag="KPG09_003231"
     CDS             1993..2664
                     /locus_tag="KPG09_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBG0967811.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2855..3652
                     /locus_tag="KPG09_003232"
     CDS             2855..3652
                     /locus_tag="KPG09_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBG0967812.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
     misc_binding    3996..4230
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4280..5299
                     /gene="pheS"
                     /locus_tag="KPG09_003233"
     CDS             4280..5299
                     /gene="pheS"
                     /locus_tag="KPG09_003233"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBG0967813.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            5316..7709
                     /locus_tag="KPG09_003234"
     CDS             5316..7709
                     /locus_tag="KPG09_003234"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBG0967814.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            7734..8324
                     /gene="zapA"
                     /locus_tag="KPG09_003235"
     CDS             7734..8324
                     /gene="zapA"
                     /locus_tag="KPG09_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBG0967815.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDVYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
BASE COUNT         3264 a          933 c         1620 g         2554 t
ORIGIN      
        1 ttcaaaaaag tatatgaaat ttatactggt aaaatgataa ataaatttta agtacaaaat
       61 agtaaaagct atgatagttt tagacccatt atatgtaaaa aatatagatg tttttggtgc
      121 aatttaaatt aaaagatata tataaaaatt taaaatattc caaacagaaa ttataaaaat
      181 tactatcatt gtctttatgc aatatttata ctataataga ataataattt agaggaaagc
      241 tctaaaaata aacaaattaa gggtcttaac agtagtgatt ttatgttaag gaggtataaa
      301 gaaagttgaa aacaatagtt agaagactta ttctctacgc agatggaatg cgtcctactc
      361 aaataatggt atctgggttt gctgcgatta ttgtaatcgg agcattgttg ttaacactcc
      421 cgatagcgtc gcagagtgga gagagtatag gcttattaaa tgctctattt acggctactt
      481 cagcggtttg tgttactggt ctagttatgg tagatacagc aacttattgg agtttatttg
      541 gacagatagt tataataaca ttgatacaaa ttggtggtct tggatttatg accgtagcca
      601 caatgttttc acttatggca cgaaaaaaaa tacaactaag agaaagatta ttaatacaag
      661 agtcattaaa tcaagctgat ttatcgggac ttgtaagact tacaagattt gtattaataa
      721 taaccataac aatagaggga ataggtgcac tggttttatc aactgtcttt attccacaat
      781 ttggtttgtc taaagggata tggtatagtg tatttcacgc aatatcagcg ttttgtaatg
      841 ccggatttga cttgatgggc agtgtaagtg gcccatttac atcgttaaat tcttatgtaa
      901 acaactttac agtatcaatg actgtttgcg cacttatagt gcttggaggg ctaggattcc
      961 ctgttgtact tgatattgta agaaaaagaa gattttcaaa attaaatgtg cattctaagg
     1021 ttgtattgtt ttcaactgct acacttattt ttgtgggtgc attatttatt tttcttatag
     1081 aatttaatca aaaagctact atggcggact tgcctttaaa gggaaaagta ctatcagcaa
     1141 tatttcaatc agtaacagca agaactgctg gttttaatac acttgattta gctactttac
     1201 gagaaagtag tgtatttgta atgataattt tgatgtttat tggagcatca cctgcatcaa
     1261 ctggtggggg tataaaaaca acaacactag ctgttttaat cattacagtt agaagttttc
     1321 tatcaggaaa atcagatata gaagcttttg aaagaagatt agctccatca actataaaaa
     1381 aatctcttgg tatatttgta atcagtatat cagcagttat ttttggaaca ttgattattt
     1441 caataactca gcctaatttt acattagttc aatcagcttt tgaggttaca tcagcacttg
     1501 ctacagttgg ttcaagttta gctggaactc caaatttaaa tgctttaggt aagattataa
     1561 taataatctt tatgtttatg ggtagagttg gttctcttac tttatttatg gctatacttt
     1621 ctggtggtag aagaaagagt caaccaatca gatatgcaga aggtaaaatt atggttggtt
     1681 aagttaccaa ggagtaatat aaatttataa tggatttaga gtagcactgt aaatctgttg
     1741 ataaaataaa tgagcctaga tgtaattata tgaagaaata aaatcaacta ataaacaaaa
     1801 ttaattaatg aaataaaaaa acagggaaat aaaatacaaa taaaaaggta aatcaaaatt
     1861 aaattaatct gtgtatataa aattatatat tgtgtataag ttttgtatgt gtaaagtata
     1921 taagttttgt atgtgtaaac aattaattta agagatatta tataaattgt gtattaggga
     1981 ggaagtttcg agatgaagca atatatagtc attgggtgtg ggagatttgg aagttcagtt
     2041 gcgtctacta tgcatctttt aggacatcaa gtaatggcaa tagacaaaaa tgaagattca
     2101 gttcaaagta tatctgacaa ggtaacccat tcacttatag tggatgttac tgatgagcaa
     2161 gcgttaaggt cattaggttt aggtaacttt gatgtagcag tagttgcaat aggttctgat
     2221 ataagggcat ctataatggc gactcttata gccaaagaaa tgggtgtaga gttgataata
     2281 tgtaaggcaa aggatgaatt acaagctaaa gtgctttata aaattggcgc agatagagtt
     2341 gtatttccag aaagagatat gggagtaaga gttgcacaca atttagtttc ggataatata
     2401 ttagaccata ttgaacttga cccagagtat tcaattgttg aaatcgtaac tccaaatagt
     2461 tgggttggca agacacttat agagcttgaa ttaagagcta gatatgagat aactgtactt
     2521 gctataaaaa caggtaaaaa tataaatgtt acaccttctc cagatgagga acttacagcc
     2581 ggaagtatcc tagttataat cggtcaaaat actagtataa cagcgataac atctggaaat
     2641 aaggggataa ttagaagaag ataatttact atttaatata tatttaattg caatgaaagt
     2701 aaagagtatc atataattat gaatagttat atgatactat ttttatttaa tcgaaggtag
     2761 tatttatttg taagattaga taaagagaag ttaaattaaa agtaaggagg ctgtgctaat
     2821 ataaaaattt ataagttatt agcatttaga taaaatgatt acaaatataa acagtaagga
     2881 taatgaaaag ttaaagtata caagagcact attaaaatca aagaatagga ataaagagtc
     2941 aaagttcata atagaaggat acagaatagt aatgcttgca cttgaatgta tggcaaacct
     3001 tgattatgta tttatcaatg aagaatttga aaataagaaa gaacatgtaa aactattaga
     3061 agatttggat aaaaaaaaca caaagatata caagactact aataaaaact ttaaagaatt
     3121 agtggataca gaaaatactc aaggaataat aggtgtagtt tcatttaaga aaaaaaaatt
     3181 aagtgaaagt ataaataaaa aagataaatt tgtattgatt ttagatagaa tacaagaccc
     3241 aggaaatatg gggactataa taaggactgc tgattctgct ggagtagatg ccataatagc
     3301 actaaaggga tgtgtcgata tatacaaccc aaaagtaatt aggtctacta tgggttctat
     3361 ttttgatatg aatataattg atgcttcaca agatgaaact gtggacatgc ttaaatcatt
     3421 ggattttaat atagtttcaa gttacttaaa tacagaaaat ttttatgaca aaatagatta
     3481 tggttcaaaa gtagcattgg tgataggaaa cgaagcaaat ggaataaatg aagaacttgt
     3541 atcaaagtct gatattttgg ttaagatacc tatatatggt aaagccgagt cgttaaatgc
     3601 tgcgataagc tctgctatac tgatgtatga aataaaaaaa tacttaattt aatgtattgt
     3661 aataaatata atgtcatgtt ataatcttga attaaatagt agagtatcaa aaatagtcaa
     3721 tatatattaa aaaaataatt aaattaatta tatattagat gtatatcata taaaaaatga
     3781 ttgagttgat tatgtattag atatatatta tataaaaaat aattgagtta attatgcatt
     3841 agatgtatat tatataaaaa ataattgagt taattatgca ttagatatat attatataaa
     3901 aaataattaa attaattatg tattggatat atattaaaaa tagtcacata atttgaatgg
     3961 aaatgatata atactaaaat aaacaatata atattgtaaa tgcaatgaaa gaggaaagta
     4021 ttttgattaa acttagtaaa gagataaaca cctaggctgg gagtgttttc taagaggtca
     4081 tgagaagttc cctctggagt aacagagctg aaattttaca gtaggctttg acgtcaaaaa
     4141 cgcgttaagt ttgttagagg tggtttgatg atttttaaat tgttaaacta ctagggtggt
     4201 accgcgaaac tatagccttt cgtccctaga cagggattga ggggcttttt ttatacaaaa
     4261 aaacgaaagg gtgatatgtg tgcaagaaaa attacttgct ttacgtgaag cagctttggc
     4321 tgaaataaaa gaagcacaaa gcatagaaag tgtagaaagt ttaagagtta agtacttagg
     4381 aaaaaaaggt gagataactg ccatacttaa agaaatgggt aaattatctg ctgaagaaag
     4441 accagtagtt ggtaaggttg ccaatgaggt aagagaaaac attgaactta gcataaattc
     4501 taaaaaagaa gaaataaatg ctattgaaaa agaaagaaaa ttaaaagagg aagtgataga
     4561 tgttactcaa ccaggaaaag ttttaaaggt tgggaagaag catccaataa ctcaaattat
     4621 agatgaagta acagatatat ttatcggaat gggattctct atagcagaag ggccagaagt
     4681 tgagactgtt gaaaacaact ttgacgcatt aaacgctcct aaagaccatc catcaagaga
     4741 tatgagcgat acattctata tcaatgatgg ggtattactt agaactcaaa catctccagt
     4801 tcaagtaaga actatgagaa gtcaagagtt accaataaaa gtaattgcac caggtagatg
     4861 ttttaggtca gactcgccag atgctacaca ctcaccaatg ttccatcaga tagaagggct
     4921 tgttgttgga aaagatgtta ctatggcaga atttaaagga actatggata tcttcgttga
     4981 aaaattgttt ggttctgata tcaaaactaa gtttagacct cacaacttcc catttacaga
     5041 accaagtgca gaggttgatg ttacttgttt caaatgtggt ggtaaaggtt gcccaatgtg
     5101 taaatatgaa ggttggatag aaatattagg ttcaggtatg gttcatccaa atgtgcttag
     5161 aaattgtgga atagacccag aagtttacag tggatttgca tttggagttg gggttgaaag
     5221 acttgcaatg cttaaatacg aaatagatga tattagatta ttattcgaaa atgatatgag
     5281 attcttaaat caattttaat taggagggta tgtagatgtt agtatcttta aaatggctta
     5341 gagactatgt tgatatagac atggatgtaa aagagttcgc tgataaaatg acaatgacag
     5401 gaactaaagt tgaaacaata gattattatg gtgaagaaat agaaaatata ttggttggaa
     5461 agattttaga aataaaacaa catccaaatg ctgataagtt ggttgtaact aaagtagata
     5521 ttggagataa agttgttcaa atagttacag gagctacaaa tatatcagaa ggagattata
     5581 ttccagtagc tgtaaatggt tctaagttac ctggaggagt tgaaatcaaa cagactgatt
     5641 tcagaggtga attatcagat ggtatgatgt gttcagcagc tgaactaggt atagatgaac
     5701 attacattga ggagtataaa agaggtggta tatatatttt agaccacgaa gattcttatg
     5761 aattaggaaa agatataaaa gatgttttag gattaaaaga tgctttaata gattttgaat
     5821 taacttcaaa cagacctgat tgtaaatgca tgatgggtat agctagagaa gcagctgcaa
     5881 ctataggaac aaaagtaaaa tatcctgaaa tcgaagtaaa agaaagtgac gaagagatag
     5941 atttcaaagt tgagatagat aatccagatt tatgtagaag atatgttgct agaatggtta
     6001 cagatgtaaa aatagaacct tctccatatt ggatgcaaag aagacttaca gaagcaggag
     6061 taagacctat aagtaacata gtcgatataa caaacttcgt aatgttagag cttggtcaac
     6121 cacttcatgc ttttgatata aatcaagtag agactggaag aatagtagta agaaatgcta
     6181 aagatggaga gaaacttgta acattagatg atgttgagag aacattagat aaagatatgc
     6241 tagttataac aaatggagaa aaatcacttg gtttagctgg tgtaatgggt ggtgctaact
     6301 cagaaataac ttctaatacg aagactgtac tttttgaaag tgccaatttc aaaccagaaa
     6361 acataagaat gacagctaaa aaagttggta ttaggtcaga agcatcttca agaaatgaaa
     6421 aagacttaga ccctaatctt gcagagatag cagcaaatag agctgcacaa cttgttgaaa
     6481 tgttaggagc aggaaaagtt ttaaaaggtg ttgtagatgt atatccaaat aaaccagaac
     6541 ctaaaaaatt ggtagtaaat cctcaaagaa ttaaccacct attaggtgta gatgtaccaa
     6601 tggagcagtt tgtaggaatt ttagaatcat tagagtttaa atgtaatttg gtagctaatg
     6661 ataaattaga aatagatgta ccaagcttta gaacagatat ggaacaagaa gctgatgtat
     6721 gggaagaaat agctagaatt tatggatttg agaatatacc ttctgtacaa ttagaaggaa
     6781 atacgacagc aggaattaaa acttcaaaac aaaaattcat ggatgcttta aaagataatt
     6841 caactgctgt aggattaaat gaaatattaa catattcatt tgtaagccct aagggagtcg
     6901 ataagataag agtaccagaa ggtaatgcta agagaaactt tgttaaatta cttaacccat
     6961 taggagaaga aacttctgta atgagaacta ctttaatacc aaatatgtta gatgttttat
     7021 caactaatgt atctcataaa atagaagaag tatctgcatt tgagtgtgga catatattca
     7081 tcccacaaga ttcagaatta cctaaagaag aaaatagaat gtgtgtaggt atgtatggta
     7141 aagatgttga cttctttgca ctaaaaggaa ctatagaaac tatacttgta aatgtcggat
     7201 ttaaaggtta tgaaattgaa cctcaagata ataatactac cttccaccct ggtagatgtg
     7261 ctaaaatagt gtataataat aaatatgtag gtacattagg tgaattgcat ccagatgtta
     7321 tagaaaacta caatctaggt caaagagttt atgtcgctga aatagatatt gactttgtat
     7381 ttgataattc tgatagaaca aagaattatg tgccattacc aaaatatcca tctacatcaa
     7441 gagatatagc tcttattgtt aaagatgatg tatttgtaaa acaaatagaa gatataataa
     7501 aggaaaatgg tcaaggatta gtagaaagct acaagttatt tgatgtttac aaaggttctc
     7561 aaatagaagc tggatacaag tcaattgcgt attcaataac ttatagaagt aaagataaaa
     7621 ctctaacaga tgaagatgta gctaaggtgc atgataagat attaagtgaa ttaagcgaaa
     7681 aattaaatgc taacttaaga tcaaattaac aactaatgtc gggggaggtt aggatgaaca
     7741 aagtaatggt taaaatccat ggcgctgaat atccaatggt tggagacaaa tcagagaagt
     7801 ttatgattag tattgctgat tttgtagaca aagaaatgga taaaataaca cgtcaaaacc
     7861 cgaaattgag cttatcagtt gctgcgatac taactgcatt aaatatatca gaccttttat
     7921 ttgaatgttc agatgagaat gagaagttga ttaaagctaa tgaagaattg agtaagaagg
     7981 ttggagcatc taatgaagag cttcaattag aaatcaagtc tttaaagctt acgatagcag
     8041 aaaaagaagc agaaaatcgt gaagctgaga ctaagatgaa agagttaatt gagattatag
     8101 aaaataaaaa gcaagaaata ttcgaactta gtaatacgac agaaggttct agagcagagt
     8161 tagatgttta taaaaacaag attgaggagt taagtactca acttgaagaa gcaaatgaaa
     8221 gagctactat agctgaaaac ttagcttctg agtttcaaaa caaagcttat gatttacagt
     8281 taaaatgtac agggttaaat aatgatgcaa aaaatgtgga ataaactaaa agatttgctg
     8341 tctcttgttt tttgagacag cttcttttaa t
//
LOCUS       DAELSY010000160         8270 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.160, whole genome shotgun sequence.
ACCESSION   DAELSY010000160 DAELSY010000000
VERSION     DAELSY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8270
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.160"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            51..206
                     /locus_tag="KPG09_003236"
     CDS             51..206
                     /locus_tag="KPG09_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967816.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            336..1349
                     /gene="aroF"
                     /locus_tag="KPG09_003237"
     CDS             336..1349
                     /gene="aroF"
                     /locus_tag="KPG09_003237"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG0967817.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKIVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1371..2435
                     /locus_tag="KPG09_003238"
     CDS             1371..2435
                     /locus_tag="KPG09_003238"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBG0967818.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2435..3748
                     /gene="aroA"
                     /locus_tag="KPG09_003239"
     CDS             2435..3748
                     /gene="aroA"
                     /locus_tag="KPG09_003239"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBG0967819.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNSTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3726..4805
                     /gene="aroC"
                     /locus_tag="KPG09_003240"
     CDS             3726..4805
                     /gene="aroC"
                     /locus_tag="KPG09_003240"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBG0967820.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDKIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQRDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4808..6004
                     /gene="pheA"
                     /locus_tag="KPG09_003241"
     CDS             4808..6004
                     /gene="pheA"
                     /locus_tag="KPG09_003241"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBG0967821.1"
                     /translation="MEDLTRYRNEIDNIDKEIIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAIDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKTISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYELEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAENNINMTKIESRPMKNASWRYFFYIDFECSIYNSKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            6051..6860
                     /gene="aroE"
                     /locus_tag="KPG09_003242"
     CDS             6051..6860
                     /gene="aroE"
                     /locus_tag="KPG09_003242"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG0967822.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRIFEILNIESAYKNFEIPKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            6872..7405
                     /locus_tag="KPG09_003243"
     CDS             6872..7405
                     /locus_tag="KPG09_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBG0967823.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            7426..8265
                     /locus_tag="KPG09_003244"
     CDS             7426..8265
                     /locus_tag="KPG09_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBG0967824.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
BASE COUNT         3403 a          832 c         1489 g         2546 t
ORIGIN      
        1 taattattaa acaagaagaa taataaatta taaaaatgga ggataatact atgaaaggaa
       61 tattaaaaga actaacagca aaactttata gcttttttta ctttatgttc tggggctttt
      121 attttagcgc tggctatttc ttttacaaaa atatatgtac ttcctttctg gtgaggcctt
      181 attttagtga ttttgagcat aagtaaattt tataaaaatg attattttat atatttatat
      241 aagatttagt gcttgataga ttattttaga gaactcatta gtgagttctc ttttttattt
      301 taaatataaa attataatat tgggaggtag taaaaatgat agtagtatta aaaatgggag
      361 cagataaaaa tgaagttaaa aaattaattg aggctattgg gagagaagga gtagaagtta
      421 atcccataga tggaacagaa ttaactgttt taggtttagt tggagatact agtaaaatag
      481 atgccaaaag aatagaagca aataagatag ttgaaaaagt tatgcatgta gtagagcctt
      541 ttaaaaaggc caatagaaaa tttcatccag aaccttcaat cataaatgtt aatggaatgg
      601 aaattggttc aaagaaaatt gctatgatag ctgggccttg ttctgttgaa acagaagacc
      661 aaatagtatc tatagctaaa gatgtgaaaa aatcaggtgc aggatttttg agaggtggag
      721 cattcaaacc aagaacatca ccatacgctt tccaaggatt gaaatatgat ggattggatt
      781 tattaaaaaa agcaaaagaa aaaacagggc ttcctatagt tacagaaata atgtcaacac
      841 aagatataga tatatttgaa gaaaatgtag atgtaattca agttggagct agaaacatgc
      901 agaactttga tttattaaaa gaattaggta aaacaaataa aacaatactt ttaaaaagag
      961 gattatctgc tacaattgaa gagtggctaa tgtcagcaga gtatataatg gctggaggaa
     1021 atgaaaatgt agttctttgt gaaagaggta taagaacttt tgaaacatat actagaaata
     1081 ctttggattt aagtgcaatt ttagctgtga agaaacttag tcatttacca gttatagttg
     1141 acccaagtca tgcagcaggt aaatcatgga tggttgactc tttatctaag gcagcaattg
     1201 cagttggagc agatggtcta ataatagaag ttcataatga cccagcacat gcactttgtg
     1261 atggaaaaca atcgattaag ccaaatgaat atgatgaatt gataagtgaa ttaaagacta
     1321 tcgcatcagc agttggtaga gaaatataat ctataaaagg atggttaaac gtggaagaaa
     1381 aactattagt aaatttaaaa gaaaatagct acaatatatt tattaaaaaa aatatactaa
     1441 agaatatagg agaagaaata aaaaaaatat attttggaaa taagattttt attgtaactg
     1501 atgaaaatat agcaaaatta tacttggata aagtaaaaaa taatttaagt gaatctggat
     1561 ttgaaattaa atctttaata ttaaaatctg gtgaagaaac aaaatcattt aatactcttc
     1621 ctaaaatata cgatagttta cttgacttta agttaactag aaaagatttg attattactt
     1681 taggaggagg ggtaataggt gatttaggtg gatttgcagc agcaactttt ttaagaggta
     1741 taagttttat tcaaattcca acatctttat tagcacaagt tgatagttct gtaggtggaa
     1801 aagttggagt tgaccttgag agaggcaaaa atttagtggg aagcttttac caaccaaaag
     1861 ctgtgtttat cgacccaaat gtattattat cacttccaga aaaattctat agagatggaa
     1921 tggcagaagt tataaaatat ggatgtataa aagacaaaac ttttttcaat aaacttttaa
     1981 gtttaaaaaa tagagaagaa gtaatgcaac atatagaata cattatacat aaatgttgct
     2041 ttattaaaaa aactgtagtt gaagctgatg aaaaagactt tggagataga atgcttctaa
     2101 actttggcca tacattaggt catgctattg agaaatacta caattttacg ggttataccc
     2161 atggagaagc tgttgccata ggaatgtata agataacttt agaaagtgaa aagataggaa
     2221 ttacaaaaaa aggtacatca gaacttataa aatcaatttt aatgaattat aatttgcctt
     2281 atgagattga attaagagat aataaatcta tagtagatgc tatatctctt gataaaaaaa
     2341 atctaggtga tacattaaaa ataatacttt taaaagatat tggagagagt attatatata
     2401 atactacttc taaatttttt caggaggtaa gataatggga agtctaaaaa tatatccaag
     2461 taaattatct ggagatgtaa aaataccacc atcaaaaagt atggcacata gggcagttat
     2521 ttgttcatct ctaagcaatg gaaaaagtag aatttctaat atagattttt ctgatgatat
     2581 aatagctact ataagagcaa tgacatcatt aggagctatt atagaaaaga aagaagatat
     2641 tttagaaatt tcgggaattt tttctaaaga aggtatttta aatagagaga atcagttgaa
     2701 tcaacctaaa ttgacaatag attgtaatga atcaggctct acattaagat ttttagtacc
     2761 tatatcttta gcatttgatg gtgtaaaaag gtttataggt agaggtaatc ttggaaaaag
     2821 accacttgat acttattatg agatatttga cagacagaat ataaaatata gttacaaaga
     2881 aaatcagtta gatttgatta tatcaggtaa gcttaaacca gatgaattta gagtaaaagg
     2941 aaatataagt tctcagttta tcacaggtct attgtttata ttgcctacac tagagagtga
     3001 ttcaaagata ataattacta ctgagctaga atctaaagga tatttagatt taactctttc
     3061 aacgataaaa gattttgggg tagaaattat aaacaataac tataaagaat ttattataaa
     3121 aggaaatcaa acgtataaag cgagagatta taaagtagaa ggagattatt ctcaaggagc
     3181 attttattta tcagcagatg ctataggaga agatattagc atattagacc ttaaagaaga
     3241 ttctcttcaa ggggatagtg aagtagttga aattttatct aggatgggaa tggaaatatt
     3301 aagagaaggc aacaaaataa aaggaataac aaatggttta aatagtactt tgatagatgc
     3361 atctcaatgc ccagatataa tacctgtatt atctgtagta gcatccttaa gtatagggag
     3421 gactacaata attaatgctg gtaggcttag aattaaagaa tgtgatagac ttcatgcaat
     3481 aaatgttgag ttaagtaaac ttggagcaaa tatagaggag aaagaggata gtttgataat
     3541 cgaaggagta agcaagttaa atggtggagt agaggtgtgg agtcacaaag accatagaat
     3601 tgctatgaca cttgcaattg cttcttgtag atgtgataaa ccaataatat taaaagattt
     3661 tgagtgtgta tcaaaatctt atccacattt ttttaaagat tttaagatgt taggaggtcg
     3721 aattgatgag tggaatatgg ggaaataatt taaaagtatc aatttttgga gaatctcatg
     3781 gaaatgctat aggtataaat atagatggtc ttccttcggg gattgaactt gaccttgata
     3841 agattgataa agaaatgaag agaagagcac ctggaaaaaa ttctatatct acttcaagaa
     3901 atgaaagtga tataccagaa attctaagtg gatattttaa tggaagaact acaggaacgc
     3961 cattatgtgc aatcataaga aatagtgata ctcgttcaaa agattatgga gagcttaaaa
     4021 atttaatgag accaggacat gcagatttca caggtaatgt gagatattct ggatttaatg
     4081 actatagagg gggaggtcat ttttcaggta ggataacagc tccattagta ttttgtgggg
     4141 ctatatgtaa acaaatttta tcacaaaaag gaatagagat aggagcacac ataaaaaaaa
     4201 ttaaaaatat agaagatatg agttttgatt atgtaaatat ttcaaaacaa cagttatcaa
     4261 atttgcaaac tttggaacta cctttacttg atttatcaaa ggaagaagcc atgaaaaata
     4321 cgattataga tgcaaaaaat caaagagatt cagttggtgg tataattgag tgtgcagtag
     4381 taggtattaa tgtagggctt ggaaatccat tttttgactc tgttgaatct actttatcac
     4441 atttattgtt ttctgttcct gccgtaaaag gtgtagagtt tggtttaggt tttgaacttg
     4501 cagatatgta tggctctcaa agtaatgatg aaatgtatta tgaaggaaat caagttaaga
     4561 gcaaaacaaa taacaatgga gggattatag gagggattac tactggaatg ccaataattt
     4621 tcaaagtggc aattaagcca actccttcaa ttagcagaca acaaaacaca gttaatataa
     4681 aagataaaaa ggacgatatt ttatatatta aaggcagaca tgacccttgt atagttcaaa
     4741 gggctatacc tgtaatagaa gcagtaacag ccattggaat ttttgatttg atgaaaggac
     4801 gataataatg gaggatttaa cgagatatag aaatgagatt gacaatatag ataaagagat
     4861 tattcaatta tttgaaaaga gaatgaatac agttcttgaa attgccagac ataaaatgaa
     4921 aaataacact actatacttc aaaaagatag agaagaaaaa gtactaagta gagccataga
     4981 taatttagat aataaagcat actcacaaga aacggtacag ttttttaatt ctataatgga
     5041 aattagtaga ggattacaaa aaagattgat agataatgat ggtgagcaga aaatcgagtt
     5101 tagtttagat tcatcaaata agaaatttga tttgtcaaat gtaaataaat ataagtcttt
     5161 aaaagatgaa ttaaataaaa aaaatatcct agtaggattt ccaggaaaat caggttcatt
     5221 tacagaagaa gcactaaata aattcttcaa caaaacaata tcaaaaaaac aatttaaaga
     5281 atttgaagat gttttcatag cacttaaaaa taaagaaata aactatggaa taatccccat
     5341 agaaaattca tctacaggag ctatctctga aacttatgac cttttgagaa agtacggttt
     5401 ttatatagtt ggagaagaat gtataaaaat agaccaaaat ttaataggga taaaagacac
     5461 aaaacttgaa gatataaaag aaatatattc tcatccacaa ggtataggac aatgtagtga
     5521 atttttaaaa caaaatagta catggaaatt gataccattt aataacacag caacaagtgc
     5581 agaacttgta agaaaacttc aagataaaac aaaagcagca attgcaagca aaaaagctgc
     5641 aagtatttat gaattagaaa taataagtcc ttgtataaat gatattgcaa acaactatac
     5701 aagatttgta gtaatcagta atcaaataca cattgaagaa gaatcaaaca aaatgagtgt
     5761 agtattttct gttgaacatg aagcaggaaa actttataaa gtacttggat actttgctga
     5821 aaataatata aatatgacaa aaatagagtc aagacctatg aaaaatgcct catggagata
     5881 tttcttttat atagattttg agtgttctat atacaattct aaagtgtacg acttattaga
     5941 attgatagaa cataatacag catattttaa atttatggga gtatataaaa ataaaactga
     6001 atagataata taaattttta tttaatttat aattattagg aggtgtaagt atgaattttt
     6061 tcggtctagt aggtgaaaaa ctctctcaca gtgtttcacc tcaaatccac aagagaatat
     6121 tcgagatatt gaatattgaa agtgcttata aaaactttga aattccaaaa gaagatatat
     6181 ctaagctaga cggtgctata aaattattag gaatacaagg agtaaatgtt actgtaccat
     6241 ataaggagcg tattatgaaa tatcttgatt ttatatcacc agaggcaaaa cgtataggag
     6301 cagttaatac aattttactc agagaaaata tgttatatgg ttacaataca gattactttg
     6361 gtcttgatag tatgtttaaa atggctaata ttgatgttca aggaaaagta gcagttatat
     6421 taggaactgg aggtgcttct aaagctgctc ttacatattt tattgattct ggtatagaaa
     6481 agctttatgt ttcaactaga aaaaaggatg ataaaaaatt attaaatagt aaagctatat
     6541 taatagatta tgaagaactt aaacatataa aaggagatat aatattaaat gctacaccag
     6601 taggtatgta ccctaatgta ggaatttcac cagtgtcaaa atctattata caaaattttg
     6661 atatattaat agatttaatt tacaatcctg gagaaacaga gtttttaaga attggtaata
     6721 gtatgggtaa aaaaacttgt gatggattat atatgctggt agggcaagca ataaaatctc
     6781 aggaaatttg gcaagatact aaaatagata atagtatttt agatgttata tataatgaac
     6841 taaaattaga atttttgtaa gggagattgt catgataaat aaaacaaaag aaaaattgat
     6901 attaataggt atgccaggta gtggaaaaac tacaattggt aaacttttgg ccaaagaata
     6961 taattgtagt ttctgtgata tggatgatta tataattcaa atatctcaaa aaagtatagc
     7021 agagttgttt tcagaaggag aagatatctt taggaactat gagactcaag catgtagaga
     7081 actttctata agtgataaaa cagtgatatc tacaggtgga ggtgtaataa aaaaggatgt
     7141 taatatggaa atactaaaag aaacaggtat aataattttt attgatagac ctatacaaaa
     7201 gattttggaa gatataaata ttaattcaag accattatta aaaaacggaa aagatagatt
     7261 gtataatctt tataatgaga gaataaatct ttataaaaaa ttttcagata tagagatttt
     7321 aaatgataaa tcattaaata atgctgtata taatattaca aatgcagtca gtgaaaattt
     7381 taaatttgac tttaaagaaa aataatctag ggggaaataa aaataatgaa tattgttatt
     7441 gtaggactag gagttatagg aggctctttt gctaaagcat taaaaaaagc tggatatgaa
     7501 aatgtatttg gagtagatgt agatttagaa actttaaaaa aagcagaaaa agcaaaaatc
     7561 ataaaaaaag gatgtactac aggtaaagag ttatttaaaa aagctgattt aattatacta
     7621 tctatatatc ctagattagt tgtagatttt ctaaataata ataaaaactt ttttaaaaaa
     7681 gggactataa ttactgatac tacaggaata aaagaaacac ttatcaatga tgtgttgcaa
     7741 attattccag atgatataga ttttatattt ggacatccaa tggcaggtag ggaaaaaaag
     7801 ggtattgatt ttgcaagtga acaagttttt aatggcgcaa attatataat tactccaact
     7861 ggcagaaaca acataaaaaa tttagagtta gttgaaaatt taattttaga gattggattt
     7921 aaaagagtaa aaaagttaac ttcacaaaaa catgatgaga ttatagcttt tacttctcag
     7981 ttacctcatg ttatggcagt tgctcttata aatagtgatg aagaaggtag agatacaggt
     8041 aaatttattg gagatagtta tagagattta actagaattg caaatatgaa tgaagactta
     8101 tggagtgaac tttttttagg aaatagagat aatttactaa aagttataga aaactttgaa
     8161 tcagaagtaa atttagtaaa agaagctata tttaacaatg ataaaaataa attgatagag
     8221 tattttaaaa aatcatcaat aagaagagaa gctttagaaa aataaatatt
//
LOCUS       DAELSY010000161         8058 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.161, whole genome shotgun sequence.
ACCESSION   DAELSY010000161 DAELSY010000000
VERSION     DAELSY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8058
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.161"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(18..1658)
                     /gene="bclA2"
                     /locus_tag="KPG09_003245"
     CDS             complement(18..1658)
                     /gene="bclA2"
                     /locus_tag="KPG09_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HBG0967825.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGANGTTGSTGPTGNTGATGANGITGPTGATGATGA
                     NGITGPTGNKGATGANGITGPTGATGATGANGITGPTGNTGATGANGATGLTGATGAT
                     GANGITGPTGATGATGANGVTGATGPTGNTGATGPTGSIGATGANGVTGATGPIGATG
                     PTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGLVGPTGATGATGV
                     AGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGATGANGVAGPIGPT
                     GPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGPIGPTGPTGANGTTGATG
                     ATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLGNNTLVTFNSSFINGTNITFP
                     TSSTINLAVGGIYNVSFGIRAILSLAGFMSITTNFNGVAQNNFIAKAVNTLTSSDVSV
                     SLSFLVDARAAAVTLSFTFGSGTTGTSPAGYVSVYRIQ"
     gene            complement(2036..2575)
                     /gene="hpt"
                     /locus_tag="KPG09_003246"
     CDS             complement(2036..2575)
                     /gene="hpt"
                     /locus_tag="KPG09_003246"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG0967826.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(2903..4210)
                     /locus_tag="KPG09_003247"
     CDS             complement(2903..4210)
                     /locus_tag="KPG09_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBG0967827.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDEKLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4635..6410)
                     /locus_tag="KPG09_003248"
     CDS             complement(4635..6410)
                     /locus_tag="KPG09_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0967828.1"
                     /translation="MMVMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDVCRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6407..6544)
                     /locus_tag="KPG09_003249"
     CDS             complement(6407..6544)
                     /locus_tag="KPG09_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967829.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6622..7191)
                     /locus_tag="KPG09_003250"
     CDS             complement(6622..7191)
                     /locus_tag="KPG09_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG0967830.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7375..8010)
                     /locus_tag="KPG09_003251"
     CDS             complement(7375..8010)
                     /locus_tag="KPG09_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG0967831.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         2284 a         1637 c          942 g         3195 t
ORIGIN      
        1 tcttttcatc atataaacta ttgtattcta taaactgata catacccagc tggagaagta
       61 cctgtcgtgc ctgaaccaaa tgtaaagctt aaagtaacag ctgctgctct agcatcaact
      121 aaaaagctta aacttacact tacatctgat gaagtaagcg tatttactgc ttttgcaata
      181 aagttatttt gggctactcc attaaagtta gtagtaattg acataaatcc tgcaagtgaa
      241 agtatggcac gtataccgaa agatacattg tatatccctc caactgcaag atttatagta
      301 ctacttgttg gaaaagttat attagttcca tttataaatg atgaattaaa tgtcactaat
      361 gtattattcc ctaaacttga agaagatact gtaaattgtg cattgtttgc tgctaacact
      421 cctgttgctc cggtcgctcc tgttggacct gttgctccat ttgctcccgt tgctccggtc
      481 gcccctgttg ctccggtcgt tccatttgct cctgttggtc ctgttggacc tattggacct
      541 gctactccat ttgctcccgt tgctcctact gctcctgttg ggcctgttgc tccatttgcc
      601 cctgttgctc cggtcgctcc tgttgctcct gctactccat ttgctccggt tggacctgtt
      661 ggacctattg gacctgctac tccattcgct ccagttgctc ctactgctcc cgttgggcct
      721 gttgctccat ttgcccctgt tgctccggtt gctccggttg gacctactgc tccatctgct
      781 cccgttgggc ctgtcgctcc tactgctccg gttggaccta ttgccccagc tactcctgtt
      841 gctccggtcg ctcctgttgg acctaccaaa ccatttgctc cggttgctcc ggttgggcct
      901 gttggaccta ccaaaccatt tgctccggtt gctccggttg ggcctgttgg gcctgttgga
      961 cctaccaaac catctggacc tgttgctcct actgctccgg ttggacctgt tgctcctatt
     1021 ggacctgtgg cacctgttac tccatttgct cccgttgctc ctatacttcc ggttggacct
     1081 gttgctcctg tatttcctgt tgggcctgta gcacctgtta ctccatttgc tcccgttgct
     1141 cctgttgctc ctgttggacc tgttatccca ttcgctcccg ttgccccagt tgctccggtt
     1201 agtccggttg caccatttgc tcccgttgct cctgtatttc ctgttggacc tgttattcca
     1261 tttgctcccg ttgctcctgt tgctcctgtt ggacctgtta tcccattcgc tcccgttgct
     1321 cccttgtttc cggttggacc tgttattcca tttgctcccg ttgctcctgt tgctcctgtt
     1381 ggacctgtta ttccatttgc tccagtcgct cctgtatttc ctgttggtcc tgtagaacct
     1441 gttgttccat ttgctccagt cgctcccata tttcctgttg gtcctgttat tccattcgcc
     1501 ccggttgctc ccgtatttcc tgttggtcct gttattccat ttgctccggt cgctccggtt
     1561 gcgcctctag gccccgtcgg tcctgttgga ccagtagcac ctgttggccc tgttggacct
     1621 acatcttgat ataaacttgg acctgaaata tcactcatat aattaatcct ccttttttaa
     1681 agttagagta ttactctata aatattgagt attactctat ataattatat gaatcgacaa
     1741 tttcttatgt gataatgtat ctatttgctt atatcaacat atatgctttg attcttaaat
     1801 ttcattactt ttttgtaaaa tcttttacaa ttattagtac tcttttacaa ttattaatac
     1861 aattaaaaaa ctcatttccc atcaattatt taaaattttt ttattatgtt ataaaaaata
     1921 gataattaaa ttgcataaaa ttaatttaat actactttaa ttacttttta aataaaaaaa
     1981 cctcaacacg attaataaaa atcatattaa ggcatccaca aaattatata attgcttatc
     2041 tatcttcatc agtaacattg aatatataag gaacatttct ataataatca ccaaaatcaa
     2101 acccatatcc aaccacaaac ttatcttcta tttcaaatcc acaataatct ggcactaaat
     2161 caacttttct tctgcttggt ttatccaaaa gaacacagca ttttaaacta gcaggatttt
     2221 tagcttttag atgttttaac acaaaatcca tagtaagtgc agaatcagta atatcgtcta
     2281 caaccaaaac atcatatcct gctatatcag tagtcacatc tgaaaccact ttaacctttc
     2341 cagtactttc ttcattgttt ccataactag atgtagtcat aaaattaact ctaagtggta
     2401 aatttatatt tctaactaag tctgcacaaa atataaagct accttttaat agagaaacta
     2461 ccataagatt tttatcttta taatcttttt ctataattgc tcctaattcc cttaatttat
     2521 ctttaatttt ttcttctgaa tacaatactt cccatttttt tgtttctata tccataaaaa
     2581 gacctccaat tgtaataatc cttcttaatt ttataagaga ttgctcactt tttcaatact
     2641 cttattcaat attagtcaat attattgagt ttttatgttc attcaacaat tattaacaat
     2701 gtatcttata ttttaagtag ttcatatatc cacactaaat tacatactat atattttaga
     2761 caaaaaaaga aaaaacttgc actaacaagt tttttctatt atacataaat ttatacataa
     2821 tcatatttta ttagagggta ctaattaaat ttatttatgc tatttttgtt atacctcata
     2881 atacaaaaca tattataaag tactagtcaa ctttattcat atcattcatt acatttaaga
     2941 ttatctcatc tgttatagac atacctttgt cacttactct acctaagttt tgtatacttt
     3001 gctcaacact tgaaccaact ataccattta gtggtggtac aaaacaatct tgcttagcta
     3061 ttatagcact ttgtatagct gcagatgctg cagaagataa ttttaatgca caaccagctt
     3121 tcgcaccgtc acatatcatt ccacttaagt cagctatcat attttctata gctccttcta
     3181 tttgcttttc agtaccattc ataagccaag agataccagc agttgctcca gtagaagcag
     3241 caacaccaca tccacacaca gcagataatc ttcctgtata gttcttaata taaccagtta
     3301 ctagatgaga tatagctaaa gcctttgcta gtttttcatc agattgtggg aattttttat
     3361 tataagcaac gattggaaga attgctgtta atccatgatt tccacttcca tttgaactca
     3421 taacaggcat ttttacgcct gccattcttg catctgaagc tccagcagtt aacatcattg
     3481 cataatttat aacatcatta cctaaaagac cttcttctat agacttttta attccatatc
     3541 cgacaccaat accagttttt tgttttaatc catattcagc catctcttca ttcattttta
     3601 ctccatctag taaaaattct atatctttaa aatcaatttc ttctacattt tttattaatt
     3661 cttgtatagt tactgtatcc attaaacttt cagctttttc tgccgcatca tttgaagctg
     3721 atacttttgg ttcattgtct aataatactt ctccatcttt ttctaagaac gtaaaattat
     3781 catgtttagt tcttattctc actttagcaa tatgattttt tccttttaac acaacttcta
     3841 taaatacttt ctctcttgta tcagcaggtg ttattgatag tggagtatta tccatataat
     3901 cctcagctat tttaacttct tcttttgtta gagtttctaa cacacttaat ccattttcag
     3961 aatggccacc tactatacct agagctgctg caatttttaa tcctaatctt tcagtacctg
     4021 gtatacctac gcacatgcca tttttgtaga tactaggact aactaacata cgattttcaa
     4081 ctatttcttc tcctaataat tcctttgcct ttgcacatgc tagagctaag gctactggtt
     4141 cagtacaccc tactgctggt ttaacttctg cctttaacat ttccactaat tcttttctta
     4201 tatctctcat tatatataaa cctcccaatt aagtttagat ttagtactca aataattaat
     4261 taaataattt cttatctata tttatagcaa aatacatgcc atatttttta tatcttttta
     4321 tatttttagt cttatattct ataaccattg gtatgactgc ttctatttat gtattttttt
     4381 tatttacata gagtatttat attctttaaa atggagaaac atatgacaat ttcgcaaaaa
     4441 gacaaaaaaa agtctcagaa tgagattcta tatcattttg agacggctca aatgtgttat
     4501 tttttaactt ataaaaacca ttttttaatt cataaaaaaa gattatgcaa aacacttata
     4561 gtataagttt aaagctttct ttaaagttat gaatttagaa aaatttaaat tttatatagc
     4621 tttttattat attactatga aattatattg tatttttcaa tttttctata taatgtagct
     4681 cttgatattc caagtgcttt agctgttaat tctttatctt ttttataatc tttgtactta
     4741 tttaaagctt ttattatctc aatcttctct agttcatcaa gtggaattat cctatcgctt
     4801 ttaacttcta actcttctac agaggtattc ttaatttttt gaggaattac attcattgtg
     4861 attattgaag aagaagacat attcacacta tattcaataa tattctgcaa ctctcttata
     4921 ttccctggcc acttatattc catcatagca tcaacaaccc tgctatctat gtcaataaca
     4981 tttttatcta atttttttga atactcttta accatatgct tgattataag tgggatatct
     5041 tcttttctct ctcttaaact tggtaatttt ataggtataa catttaatct ataatataaa
     5101 tcttctctaa aagtgccatt ttgaaccatt ttttctaaat ctttatttgt agcagcaata
     5161 attcttacat ctattgagac atttgattta cttccaattt tattaagttc cttttcttgt
     5221 aatactctca ataactttga ctgtaaatgc aaactcatat ctcctatttc atctaaaaat
     5281 atacttccct tatgagctac ttcaaacatt ccaagctttc cacctttttt agctccagta
     5341 aaagctccct cttcatatcc aaataattca ctctctaata aattgtctgg tatagcagca
     5401 cagtttacag ctataaaagg attatctact ctattgctat ggttgtgtat ggctcttgca
     5461 aatagctcct tacccgttcc actttcccca gtaataagta ctgttgatgt tgatttagaa
     5521 gctattaggg cttccttttt agaatttctt atagctacac tttctccaat tatgttttcc
     5581 aattttattc tataatcttt attcatctta ttgtagtttt ttatagcatc tcttttatct
     5641 ataaaatcaa taacatatcc ctttaactca ttttttaata ctattttatt gatgttgtat
     5701 attcccttcg aatcatatcc gtatctttta taaaaaaaac tacacgattt gtctttctta
     5761 aagttattta tagaaggttt ctttataaaa tctaattcga caaatatgtt tttgccagtt
     5821 atactatcct ttatattgaa aagctcttta aattttgaat tatatttatc aatttttcca
     5881 tccacatcaa tagacacaac agctttgtcc atattgttta atagtgtctc taattttttc
     5941 ttttctaatt caagctcata agttttaatt tgtgctttta atttattaga tattaagtct
     6001 gccattttgc ctaaaaagtt tataagacca cttttattat tttctataat ttgtgcttgt
     6061 tcttttgtaa aggctataag gcctataacg ccatgagaat cgccatcaca tataatagga
     6121 caacacacct ctgcaaactc tacacaagcc ccacttttat agcattccct acatacatca
     6181 ctactctttg ggtcatcaat tagtattgtg atctgttctg taagtgattt tttaaaagcc
     6241 gtatatttat ctactttctc acctattttg tctatatata ctcctgtacc tgcaatccta
     6301 aataactcct tatttactat agttacatct atattgagta ctgatactat agcttcagct
     6361 atattttgaa tatcagattt tatatattct aaacctatca taaccatcat tccttatttt
     6421 tctttaacat aagtatttgt cttatttctt taagaatcaa gtatatctta tttaaagtaa
     6481 attggattga catcaggatt acaaagagca taacaattat tattaactca taatttattt
     6541 ccatctaaac atctccttat aacaaaagac taggcttaac ctagtctttt atattatatc
     6601 catatttata ttttaactca tttatcttct ttttcccaca taaatatatt ttaatttatt
     6661 tgactcttta atcataccat caaagtttaa tcttgaaaat atattaaata cttcatgtgg
     6721 atagtgaatc tttgcaacag aaccacaaaa tttatgttta agataattta acttaacttt
     6781 taaattcttt aaatttaaat cctctaatat aacctctcca ttgcccttta acactctttg
     6841 tatttcagat gaagcttttt cacaatctgt aataacatta aattggtcaa ctattattat
     6901 cttgttgaac tgattatcat caaatggtag aaactctaac ttacctttaa ctacattgca
     6961 attttcatta tacactagag aataacagac atcttcataa ttactattct ccagtatagt
     7021 tacagaagtt ccaagacctc tcaatctctt gcctaggttt cctatatgac caacaagcaa
     7081 tattttatca cctacattat actcaattgt atccaaaaaa cattctaatt catcagatgt
     7141 atatttagct ttaactggct gtttatttat ctgacataat atgcttttca caatgcttca
     7201 cctcttcatt tcctcaatta taaattatgt acagattaca ttataataaa tggcgagcaa
     7261 ctattcaaga gatatttcat tagtattttt ttaaacaatc tttatacatt ttaatatgat
     7321 attataccaa atttattagg attttattca taaaaatcag tctatttttt atgtttattc
     7381 attgttattt tcatccaatt tttttatttt cgatatggat acttcatatg ccattttttt
     7441 gaccacatta ttttcatcta tttttttctc atattctcta ctttgaaccc taccccaaag
     7501 ctgtattcta tcaccaacct ttagattctt tgcaaattta gcatttctac cccaaactat
     7561 tgatggtata taatcagatt tattataagg tctgtttata gcaactaata aatcggttat
     7621 ttcccttcct aaaggtgtct tcctatacac aggttcctta catacatatc catctaaaaa
     7681 tatactatta gggtctttat tttcctcgtc tatgcttttt atttctctga caaacactgt
     7741 aagcactaat ctatttttat tatttaatgt tttgttatat gatcttaatt ggcctacgac
     7801 attgactaaa ttttgtttat caaggtcaat atcttggaga agtctttctg atacagtcat
     7861 tggaagtata tcaaaagaat cgcttagtct gtttattttt attttcatat tataaaattt
     7921 ttctccaaat atctcatggc taaattctag cttattgtct aactcccctc ttaagtttac
     7981 tacgttattc tcatctttaa cagctatcat attttatccc tccacaattg ttaattctta
     8041 tatttctggt atcagctt
//
LOCUS       DAELSY010000162         8047 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.162, whole genome shotgun sequence.
ACCESSION   DAELSY010000162 DAELSY010000000
VERSION     DAELSY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8047
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.162"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            159..923
                     /locus_tag="KPG09_003252"
     CDS             159..923
                     /locus_tag="KPG09_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HBG0967832.1"
                     /translation="MQIIDININNIDSRHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     AFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKGFCLADTSEPNYELLYFPFTDNAPIPCF
                     KNCVKKRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKI"
     misc_binding    1146..1359
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1613..2647
                     /locus_tag="KPG09_003253"
     CDS             1613..2647
                     /locus_tag="KPG09_003253"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBG0967833.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKNI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEVL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2689..3906
                     /gene="argJ"
                     /locus_tag="KPG09_003254"
     CDS             2689..3906
                     /gene="argJ"
                     /locus_tag="KPG09_003254"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBG0967834.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKENDVLVASTGVIGKPLNINLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3921..4781
                     /gene="argB"
                     /locus_tag="KPG09_003255"
     CDS             3921..4781
                     /gene="argB"
                     /locus_tag="KPG09_003255"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBG0967835.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGMDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            4811..5998
                     /locus_tag="KPG09_003256"
     CDS             4811..5998
                     /locus_tag="KPG09_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBG0967836.1"
                     /translation="MNNNNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            6107..7129
                     /gene="argF"
                     /locus_tag="KPG09_003257"
     CDS             6107..7129
                     /gene="argF"
                     /locus_tag="KPG09_003257"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBG0967837.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAYVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQKIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7256..7873
                     /locus_tag="KPG09_003258"
     CDS             7256..7873
                     /locus_tag="KPG09_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBG0967838.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     TVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
BASE COUNT         3281 a          763 c         1490 g         2513 t
ORIGIN      
        1 tttaattata tatgcaattt gataattcaa ataatctaaa aataatatta tgtaaaataa
       61 aaatatatat gacatgatgt tgttgtattc aaaatattaa gataaaaata tatagaatat
      121 atttaaaaat tattatgtaa ttagaaaagg agataagtat gcagattata gatataaata
      181 taaataatat tgacagtaga catatatgct gttcaatttc tgaaaaaaaa ggagaaaatt
      241 gtgttttttc taaaaaagct tggatgaaaa ataattttga taaaggtcta gcctttaaac
      301 gatttgatgt aagaggtaaa gcttttatag aatatattcc agcagaaaat gcatggtgtc
      361 ctattactgc ggacaattat atatttatta actgtttttg gatatctgga aaatttaagg
      421 gtcaaggata ttctaatcta ttgctacaag agtgtgttga tgatgcaaaa tcaaaaggta
      481 aatatggatt agttgtgctt tcgtctagta aaaaaatgcc ttttttatct gatgctaaat
      541 atctaaaata taagggcttt tgtttagcag atacatcaga accaaattat gagctattat
      601 attttccctt tactgataat gctcctattc catgttttaa gaattgtgta aaaaaaagaa
      661 aattaaatga gaacggtata gttttatatt acagtaatca gtgtccacat acagaaaaat
      721 atgctcctat tttgaaaaaa attgcaaatt ctagaggtat tgatgtaaat cttataaaat
      781 tcgaaacagc aaaagaagca caaaatgctc ctgcaccatt tactacatac agtctttttt
      841 ttaatggtga atttgtaacc aacgagatac tttctgagaa aaagattgag aaatttttag
      901 ataccaacat taaaaaaatt taactaaaaa tagatgtaaa tattgtattt tgagattaca
      961 ttattttata atccaatata ttttggaaat ttcttaagtt gatgaatgat aatataaaga
     1021 ttataactca atttagatac aaaatgctgg aaattaacca tatagaaaat atttccaaaa
     1081 atttaaaaat tatattgaca aagttaaaaa aataaactat catataaaac aataagaaaa
     1141 taaattgaaa tcctgtgaaa aagagagtag cataaagcta gttgatagag agctgggaat
     1201 ggtggaagcc tagtaacgaa ttttattgtg aatagagctt tggaggagat taccagaagt
     1261 aatagtatgg taattggtga atctcgcctt aagagattag agtgggtaag attatcttat
     1321 caattagagt ggtaacgcgg gtgtatattc gtctcttggt ctttgaccaa gaggcttttt
     1381 taattttaaa tgtacatttt gtagatactt tttaatttga gttttaatga ttattgtaat
     1441 tttaccaatg agagtggtac cgcggaaaat cgcctcttag tatttataat gctaaggggt
     1501 tttttgtttt aattagaata ttttaatcta catattatca aatgattatc atatagtaaa
     1561 gtggaaagat agaattttaa tactatttga atataaaaat ggagggaaca aaatgaataa
     1621 aataaaagtt ggtattattg gcgcaactgg atatgtcggg gcagagttga ttagattatt
     1681 aatgaatcat gataaagtcg aagtgacagc aataggttca aattcgtatg ttggaaaaaa
     1741 tatagttgat atatatccaa gtataggata taaaaataac atgatatgta tagaaaatga
     1801 aaaagttata gatatgtgtg atgttgtgtt tacagccctt ccacatggag ttagtgaaaa
     1861 gtttgtaata aaagcaatta aatcgaaaaa gaaagttata gatttaggtg ctgactttag
     1921 aataaaagat gaagaagtat atagtaaatg gtatggagta tcttttatag ataaaatctt
     1981 acataaaaaa gctgtatatg gattaagtga aatatacaaa gaagatataa aagatgcaga
     2041 tataattgca aatcctggat gttatccaac tagtatttct ttaccattga tgccattact
     2101 aagttctaaa ttgataaaaa ataataatat aataattgat tctaaatcag gtcttacagg
     2161 tgctggtaga gaattatcga ttagtagtca ctttacggag gttaatgaaa atatcactgc
     2221 atataaaata gggaaacaca gacatactcc agaaatagaa caaaatttaa gtgaatcctg
     2281 caaagaaaaa gtaagtgtag tttttacacc aaacttaata ccagtaaata ggggaatctt
     2341 atcaactata tattgtacaa aagaagataa tatttctata aatgatatac atagaaaatt
     2401 aactgattat tatgaattta aagaatttat agaagttctc ccattagata aggtagcttc
     2461 tttaaagaat gttaggttct caaataaatg tgtcatatca ttacatgaaa atggagatac
     2521 attgataatc tgttcagcta tagataatat gattaaggga gcagcaggtc aagctataca
     2581 aaacatgaat ataatatttg gtattgaaga aaatacaggt ttaaaaaata ttgcaccatc
     2641 attttagtaa tgaaaaaata ctaaactcat ataattagga ggataaatat ggagattata
     2701 aagggtggag ttacagtttc agaaggattt tttgcttcag ggattcactg tggcttgaga
     2761 aaaaataaag aaaaaagaga tttagctttg gtttattcag atgttttatg tgatgcagca
     2821 gcagtagtaa ctcaaaataa agtaaagggc aatcctgttt atgttacaca ggaacacatt
     2881 aaaaatggaa aagcacaagc tatcatagta aatagtgcta atgctaatac ctttaatgga
     2941 aaagaaggac ttataaatgc ttataaaatg gctaaattca ctagtgataa attaaatatt
     3001 aaagaaaatg atgttttggt tgcatctaca ggtgtcatag gaaagccatt aaatataaat
     3061 ttaatagaag aaaatatgga tgaattagtg aataatctaa gtaaacaagg tcatattgga
     3121 gcaagagaag ctattatgac aactgatatt ataaaaaaag aaatagcagt agcaataatg
     3181 tatggagata aaaaaataac tattggaggt atggcaaaag gttctggcat gatacatcct
     3241 aatatggcaa caacgttagg atttataact acagatgcaa atattgatgg agttttgcta
     3301 aaagaagctt tgaaaattgc tgttgataaa agttttaata gagttagtgt agatggagat
     3361 acaagtacta atgatatggt gatgattcta gcaaatggaa aagcaaaaaa tgatagaata
     3421 aacaaaaaag atgaacatta tcaagtattt ttgagtgcac ttacatatgt ctgtatagaa
     3481 cttgctaaac ttgttgcaaa agatggagaa ggatgtacaa aactaattga atgttatata
     3541 aatggagcat taagtgaaga acatgcagta aaattagcaa aaacagttat atcttcttca
     3601 ttagtaaaga cagctgtatt tggagctgac gcaaattggg gtagaatact gtgtgcttta
     3661 ggttatgcag gtgaagaaat agatatggaa aaagttgata ttatttttga gagtatgaaa
     3721 ggatatattg aagtatgtaa aaatggaaat ggattagatt ttaatgagga aaaagcaaaa
     3781 aaaatccttg aagatgacga aataagtata ttagtagatt taaatatggg caatgccaga
     3841 ggaaatgcat ggggatgtga tttaagctat gattatgtaa gaataaatgg aagttataga
     3901 aattaggagg atatacttta atgattaata tagaaaaagc aaatacactt atagaagctt
     3961 taccatatat agaaaaacat cagggaaaga ctattgtagt taagtatggt ggaagtgcaa
     4021 tgaaaaaaga tggtctaaaa gaatctgtta tggaagacct tgtattaatg agttatgttg
     4081 gaatcaatat agttttagta catggtggag gggcagaaat caataagatg ctagcaaaag
     4141 tggatataga aagtaaattt gtaaatggtc ttagatatac agatgaagag accatggaaa
     4201 ttgtaaaaat ggtacttgct ggaaaggtta ataaagacct agtaaataaa atacatacaa
     4261 aaggtggaaa agcagtaggg ctttgtggaa tggataataa tatgatttta tgtgacccat
     4321 ataaaaatta tgaacttgga tttgtaggag aaatcaaaaa agtaaatgtt gagcttatag
     4381 aatcttgtct aaagtcaggt tatatttcag taattgcaac tataggtgtt ggagatgatg
     4441 gagagactta taatataaat ggtgacacag cagcttctgc tatagctaaa gagttaaatg
     4501 cagataaatt gatacttctt acagatgtcc ctggtttgtt aagagagcca gatgaagaaa
     4561 aatcattaat aacagaagtt atattagagg atgttgataa attatttgaa gaaggtataa
     4621 ttactggagg tatgatacct aaaatagagg gatgcgtaga tgccttaaat aacggagtaa
     4681 atagggtaca tattttggat ggaagagttc cacattctat aataacagag ctatttactg
     4741 atagtggaat aggtacatta ataagaaaag aaaatgaata aaattataag tttaagaggg
     4801 gagaataaga atgaataaca acaatacaat ttcaaaatgg aatgaatatt ttatagatac
     4861 atataaccaa cccaattttg tcatagatta tggtgaagga agttgttttt ttgatactaa
     4921 tggcaataaa tacatagatt ttacaagtgg atatggagta tcttctcttg ggtatagtaa
     4981 tagtaattta aagaatgctc taaaagagca agtagataaa ttattacata cttcaaattt
     5041 atattttaat gagccagtgt tgtattcagg agagaaaata attaattcaa gtggaatggc
     5101 taaagtatat ttttgtaatt ctggaacaga agcaaatgaa acagcattca aaatagcaag
     5161 aaagtatagt agtgacaaat atggtaatgg tagaggaact atcataagtt taaaagactc
     5221 ttttcatggt agaacaatga tgtctttaat ggcaactggt atggacaaat accataagta
     5281 tttttatcct cttcccgaag gttttaaata tatagaaaga aataatattg aagatttaaa
     5341 aaataatctt gattccacag tttgtgcaat aattcttgaa gcgattcaag gcgaaggtgg
     5401 agttaatgtt cttgaaaaag attatgtttt ggaaatagta aaaatatgtc aagaaaagga
     5461 tattgtagtg atttttgatg aagtccaatg cggtatagga agaactggta aactttttgg
     5521 ctatgaatat tttgatgtaa aaccagatat agttaccgta gcaaaagggt tgggagcagg
     5581 aataccagta ggaggagtat tggtaaataa aaaactttca aaagtactag gaaaaggaga
     5641 ccaaggtaca acttttggag gtaatttatt agctatggtg gcagcttcag ttgtattaga
     5701 tgagatatct aaagatggtt tttataatga agtattagaa aaaggaaatt atataagaaa
     5761 atctattgaa agttttaata ataaagttgt attaaagaca aaaggtattg gtctaatgat
     5821 aggaattgaa actaatattg aatctagtat tatagaagag aaagctagaa aaaaaggatt
     5881 gcttattttg actgctggaa aaaatgtatt gagattttta ccaccattaa caatcagtta
     5941 taaggaaata gatgaggctc ttgaaatcct aaaagatata ttactagaga ttaactaaat
     6001 attcaaaaaa tagttaatta aagataaaaa ttagaagtaa ataaaaaagt aaagtgctaa
     6061 caagtttaat tgaatttaag aagtttataa gaaggggcga attaatatga ataatataag
     6121 tttaaaaggt aagagttttt tgacattaaa ggattttaca aaagaagaga ttagatattt
     6181 attaacttta tcaagttatt taaaaactaa gaaaaaagtt ggcataaaag gcgatttgct
     6241 acaaggaaaa aatatagctt tattatttga gaaaacatca acaagaacta gatgttcatt
     6301 tgaagtagca gctcatgatg aaggagcata tgtaacttat ctaggtccaa atgattgtca
     6361 tatgggtaaa aaagagtctg tatcagatac agcaaaagtt ctaggaagat tctatgatgg
     6421 aatagaattt agaggcttta aacaagaaac agttgaaagt ctagcaaaat attctggtgt
     6481 acctgtttgg aatggactta cagatgaata tcatccaaca caaatactgg cagatttttt
     6541 aacagtaatt gaaaatgtag ataaagattt gaataaagtt aaatttgtat atgttggtga
     6601 tgctagaaat aatatgggaa actcactgat gataggatgt gctaaaatgg gaatacattt
     6661 cgtagcacta gcacctaagg aactttggcc aaatgaagaa ttggttaagg aaatgaagga
     6721 aatagccaag aaatccaaag gagatataat gttgactgaa aatattgatg atgttaaagg
     6781 tgcagatgtt atatatacag atgtatgggt ttctatggga gaagaggagc aatatgaagc
     6841 aagaataaat caacttaaag attatcaagt aaatatggat atgataaaga aaactgaaaa
     6901 tgaaaatgtt atatttttac actgtcttcc agcatttcat gatttagaaa ctaaggttgc
     6961 tcagaagatt tatgaaaagt tcggattatc agaattagag gttacaaacg aggtatttaa
     7021 ttcaaaatat tcaaaggtat ttgaagaagc tgaaaataga atgcatacaa taaaagctgt
     7081 tatggtttct actatgactg atgtaaatat gaacaataat ttaatataga tatgttttaa
     7141 tatagacttt actttaatga aaatttataa aatattttaa atatattatt caaaaactct
     7201 cattatatgt taaaataact tatgtatcaa caaattaaga gggaggattt gagatatgaa
     7261 aaaaatcata ttagctttaa caactttttt attagcaata tctttaacag catgttcaag
     7321 tgcaaaacca gaagatacaa ttgatagttt ttttaacagt gcgaaaaagt ttgactttga
     7381 aggcatgaat aaggtaatgg aaaataatga cgaaaaatat aaggatatat taaaggaatt
     7441 agatacaaaa gacccaaatg cccaatatgt tttagattat ctaaaacaga atgcatctaa
     7501 aatcacgtat acaataaaag acagcgaggt taaaggtgat aaggctacaa tcaaagtaga
     7561 atgtaaattt atagattcaa caccattact tcaagaaata gtagctgagg catttacaaa
     7621 gatgattggt atgagttttt caggacaaga cttaactgat gagaaaacaa ctgaaatgtt
     7681 agtatcaatt atgaaagaaa aacaaaaaag cgttaaagag acttatgtta caaaaactgt
     7741 agagtttgaa tgttctaaaa aggataataa atggataata agttcagtaa atgatgcagt
     7801 agctgatgta ttgttatcta atcttgttac agcaggacaa gaattttcta actctatgaa
     7861 tacacttgaa taatattgtt taggtgtagt aaagtaataa atatatgatt atttttataa
     7921 ataaacatat aaatatttat gaaggatacc atttttggta tcctttttat taatataatg
     7981 aaatacatgg ttaatttcta tattgtcaag aaactatgat atatgttata agtctatact
     8041 atttaaa
//
LOCUS       DAELSY010000163         7936 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.163, whole genome shotgun sequence.
ACCESSION   DAELSY010000163 DAELSY010000000
VERSION     DAELSY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7936
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.163"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            27..101
                     /locus_tag="KPG09_003259"
     tRNA            27..101
                     /locus_tag="KPG09_003259"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="KPG09_003260"
     tRNA            116..200
                     /locus_tag="KPG09_003260"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="KPG09_003261"
     tRNA            210..293
                     /locus_tag="KPG09_003261"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="KPG09_003262"
     tRNA            317..391
                     /locus_tag="KPG09_003262"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="KPG09_003263"
     tRNA            416..492
                     /locus_tag="KPG09_003263"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            502..577
                     /locus_tag="KPG09_003264"
     tRNA            502..577
                     /locus_tag="KPG09_003264"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Gln,seq:ttg)
     gene            586..661
                     /locus_tag="KPG09_003265"
     tRNA            586..661
                     /locus_tag="KPG09_003265"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Lys,seq:ttt)
     gene            664..752
                     /locus_tag="KPG09_003266"
     tRNA            664..752
                     /locus_tag="KPG09_003266"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Ser,seq:tga)
     gene            756..831
                     /locus_tag="KPG09_003267"
     tRNA            756..831
                     /locus_tag="KPG09_003267"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Phe,seq:gaa)
     gene            838..911
                     /locus_tag="KPG09_003268"
     tRNA            838..911
                     /locus_tag="KPG09_003268"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Met,seq:cat)
     gene            926..1002
                     /locus_tag="KPG09_003269"
     tRNA            926..1002
                     /locus_tag="KPG09_003269"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Ile,seq:cat)
     gene            1020..1096
                     /locus_tag="KPG09_003270"
     tRNA            1020..1096
                     /locus_tag="KPG09_003270"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:His,seq:gtg)
     gene            1105..1180
                     /locus_tag="KPG09_003271"
     tRNA            1105..1180
                     /locus_tag="KPG09_003271"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Lys,seq:ttt)
     gene            1188..1261
                     /locus_tag="KPG09_003272"
     tRNA            1188..1261
                     /locus_tag="KPG09_003272"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Cys,seq:gca)
     gene            1269..1345
                     /locus_tag="KPG09_003273"
     tRNA            1269..1345
                     /locus_tag="KPG09_003273"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Arg,seq:acg)
     gene            1358..1433
                     /locus_tag="KPG09_003274"
     tRNA            1358..1433
                     /locus_tag="KPG09_003274"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Val,seq:tac)
     gene            1509..1922
                     /locus_tag="KPG09_003275"
     CDS             1509..1922
                     /locus_tag="KPG09_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967839.1"
                     /translation="MYIVIGNEIVDSEELKIIIDENSKFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLSSLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2231..5662
                     /locus_tag="KPG09_003276"
     CDS             2231..5662
                     /locus_tag="KPG09_003276"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBG0967840.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5904..7832
                     /locus_tag="KPG09_003277"
     CDS             5904..7832
                     /locus_tag="KPG09_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG0967841.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3101 a         1017 c         1596 g         2222 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      541 catgtatgcg taggttcgag tcctgcatcc ccagccaaac agtatggtct attagctcag
      601 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
      661 aatggagagg tgtccgagtg gtttaaggag ctggtcttga aaaccagtga cttcgaaagg
      721 ggccgtgggt tcgaatccca ccctctccgc caaatgccca gatagctcag tcggtagagc
      781 aggggactga aaatccccgt gtcggtggtt cgattccgcc tctgggcacc aaaatgtggc
      841 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      901 tattaccgct actaaaattt aatgtgggcc attagctcag ttggttagag cgcccggctc
      961 ataaccggta ggtctggggt tcgagtccct gatggcccac cacattaaat taacttatgg
     1021 tgggtatagc tcagttggtt agagcgccag attgtggctc tggaggtcgt gagttcgact
     1081 ctcattatcc accccaactt atatggtcta ttagctcagt cggtagagca cctgactttt
     1141 aatcagggtg tcccgcgttc gagtcgcgga tagatcacca aaaaggcggc gacatagcca
     1201 agtggtaagg cagtggactg caactccttg atccccagtt cgaatctggg tgtcgcctcc
     1261 aataaaatgc gctcatagct caactggata gagtgtctga ctacgaatca gaaggttagg
     1321 ggttcgagtc cctttgggcg caccatatga atagcgaggg attatagctc agctgggaga
     1381 gcacctgcct tacaagcagg gggtcacagg ttcgagccct gttaatccca ccagtgcaaa
     1441 cctctagtaa aactactaga ggttttattt ttgattgaaa aagtttttat atttaggagg
     1501 taagaaattt gtatatagta ataggaaatg aaatagttga tagtgaagaa cttaaaataa
     1561 ttatagatga aaatagtaaa tttaaagttg aaaaagattt aagtaaaagc acaaaaagag
     1621 aagatgtaat agcatatcag ttaagtatag atttaaatta cctagattct cttataaatg
     1681 agcaatgtaa tttgtccagt ttatctgatg aagagaaatt tgatgaatat atgactttgt
     1741 ctgatgaact tgcactggat ttagaagagt taatgccaaa atatacaata ataaatgcta
     1801 gagcatataa attagacgag gtagatggaa ttgtaaaaat aatactagct gtagcgtatg
     1861 cagatttagg gcacttaaaa ctttctgatg tagtaaagag gctatctaga caagtagatt
     1921 aggatatgaa tatgaactat attaaaaaaa tgtataaaat cttacgtaaa gatgaaaata
     1981 attatgtaaa catagaaaaa aacagtataa aatcaagtca attgtagaaa ttttcacata
     2041 aattcatctg aaattctata aaaaatgcct taattttaat gaggtaaatc gagtttacac
     2101 agtctaaaaa ttacttatat ttataaattt atcttattaa ttaaggaact ttcttaaaca
     2161 tcttcaaaaa taaagtattt tagtctataa tctttaatat tacacaaaca ataaaggaga
     2221 agaatagagt atgcttaaaa agtttaataa aatactagtg gctaatagag gcgaaatagc
     2281 tataagaata tttagagcct gttcagaatt ggggataaaa agtgttggta tatacagtaa
     2341 ggaagataag tatggattgt ttagaacaaa agcagatgaa tcatatttga taggagaagg
     2401 taaagggcca atagatgcat atttagatat ggatggaata atagacctag caaagagaaa
     2461 aaaagttgat gctatacatc caggatatgg ttttctagcg gagaatgcag aatttgctcg
     2521 taaatgtgaa gagaatggaa ttacttttat aggaccatca tctaaggtaa tgaatatgat
     2581 gggggataag attaactcta aaaaaatcgc aaaagaagtt aatgttcaaa ctataccagg
     2641 agtagaaaaa gcaataagaa gtactgaaga agcaaaggaa gttgcaaaca aaataggata
     2701 tcctgttatg ataaaagcat ctaatggcgg tggcggtaga ggtatgagaa ttgtacatcg
     2761 cgaagaagac ttagaacttg aatatgaaac tgcttgtagt gaatcaagaa aagcttttgg
     2821 agaagatatt atatttatag aaaaatatat agctgatcca aaacatatag aagttcaaat
     2881 attaggtgat aattatggaa atatagttca cttatatgaa agagattgtt cagttcaaag
     2941 aagacatcaa aaaataatag aatatgcacc agctttttcg ttagatgata aagtaagaaa
     3001 agagatatgt gaagatgcag ttaagttatc aaaacatgtg ggttattcta atgcgggaac
     3061 attagaattt ttagtagatg caaatggtgg gcattatttt atagagatga acacaagagt
     3121 acaggttgaa catactgtaa cagagatggt tacaggaata gatatagttc aaagtcaaat
     3181 actaatagcc caaggttaca gcttagatag tgaagaaatt aatataaaat cacaagatga
     3241 tgtggaaata agaggttact ctatccaatg tagaataact acagaagacc ctaagaataa
     3301 attcatgcct gatacaggta aaattcaagt ttatagaact ggttcaggat ttggtataag
     3361 actagatgga ggaaatggat ttacaggggc aaacataagt cctcattatg atagtttatt
     3421 ggttaagaca atatcttggg atagaacatt ccaaggagca ataaataaaa ctataagatc
     3481 tataaaagaa cttagagtta gaggtgttaa aactaatgta ggattcctag ttaatgtttt
     3541 aaataatcct atattctcaa atggtaaatg tagtactaag tttatagatg aaaatccaga
     3601 tttatttgaa ataacagaaa gtaaagacag aggaacaaaa ttacttcaat ttataggaga
     3661 tgtaattgta aatgataatg cttgtaaaga aaaaccacta tttgatgcat tacatgaccc
     3721 aagaatggat aaggatggtt caaaatcaga aggaagtaaa atacttttcg ataagttagg
     3781 taaatctgca tatatagaaa agataaaaaa tgataagaaa ttactattaa cagatacaac
     3841 aatgagagat gcacatcaat cattattagc aacaagaata agaacatatg acttattaaa
     3901 agctgctaag ccaacagaaa aatatcaaaa ggatttattc tcacttgaaa tgtggggagg
     3961 agctacttat gatgttgcat atagattttt aaaagaatct ccttggcgta gattacaaaa
     4021 gctaagagaa gaaataccaa gcataatgtt ccaaatgttg cttagagcat caaatggagt
     4081 tggatataaa aactatccag ataatgtaat agaagaattt acaaaagaat ctgctagaca
     4141 aggtatagat gtatttagaa tatttgattc attaaactgg gttgaaaata tgaaaccatc
     4201 tattaataca gctttagaaa ctggaaaaat tgttgaagca actatgtgtt atacaggtga
     4261 tatactagat aagactaaaa ctaaatacaa tctagaatat tatattaaaa tggcacaaga
     4321 attagaaagc ttgggagcag atataatagc aataaaagat atgtcaggtc ttcttaaacc
     4381 atattcagct tacacactag ttaaagagtt aaagaaaaat gtaaaagcac caatacattt
     4441 acacactcat gatacaagtg gaaatggtgt agcaacttgt ttaatggcgt cagaagctgg
     4501 agtagacata attgatgctg cattagaatc tatggcagga cttacaagtc agccatcgct
     4561 taatgctata gtagaggcac ttaagaatac agagagagat acaggaatag atttatttgg
     4621 atatgatgag ttaggaaaat attataagga tttaagaaaa gtttataata aatttgaaag
     4681 tgatttaact aattcttgtg cagaaatata taattttgaa ataccaggag gccaatatac
     4741 aaacttaaaa cctcaagctg atagtttagg attagttaat agatttgatg aagttaaaga
     4801 aaaatataaa gaagctaatg aagttgtagg agatataata aaagtaactc catcttcaaa
     4861 agttgtagga gacttagcta tctttatgac taaaaataaa ttagataaag acaatataat
     4921 agaagaaggt aaaaatttat cattcccaga ttctgttgtg gattactgta aaggtatgat
     4981 tggtcaacca gaaggtggaa taccaaaaga tttacaagag gtagttctaa aaggtgaaga
     5041 agctataaca gtaagaccag gttcattatt accagcagaa gattttgatg aaatagctaa
     5101 atatctaaat gaaaagtatg atataaatgc taatataaga aatgttataa gttatgcttt
     5161 atatccaaaa gtatatgaag attatatcaa acatctacaa cattataatg atatatctaa
     5221 acttgaaagt gatgtattct tctatggatt aaataaaaat gaagaatgtg aagtagaaat
     5281 agaagaaggt aaggtattaa caataagatt agttgaaatt ggtgaagtta aggaaaatgg
     5341 atttagaact ataggatttg agttaaatgg aatggttaga gaagtagaaa taaaagataa
     5401 gaatttctct ggaaaaatta ataatgttga aaaagctgat atgaatgacc ctcttcaaat
     5461 tggagcaagc ataccaggaa aagttataaa aataatggtt aaagaagaag atgaagtaaa
     5521 agcaaatcaa ccattaatcg ttatagaggc tatgaagatg gaaactataa tagttgctaa
     5581 gactgatgga gtaattaagt caatcaaagt aaaagaagat gatatggttg aagataaaca
     5641 attattaatg ataatgaagt aatttaatta aaataaatta aaaagcactc tgaaatgttt
     5701 aataaaaaat gttttagagt gtttttattt attttaatta aatatttgtt aaaaaaaaga
     5761 ggacaattac ctccagatgt agaaataaag ctagtaagga aaatgttatc taaaaattct
     5821 aaatatagta aatattttcc ataatatttc ttaggaaata acagcaaaga taaaatacat
     5881 aaatttaaaa ggtgggtgct tatatgaagg tttatgatag taaaatgttg agaaatgtag
     5941 cagtattagg acatagcgga tgtggaaaaa caaatctaat cgaaactata gcttacacag
     6001 caaacactaa taagatacct aaattaacag acaaggtaaa tatgacttat agcatgggat
     6061 taatacctat agagtataat gattataagt ttaacttatt ggatacccca ggatattttg
     6121 actttagcgg agacgttgtg tcatctctta gagcaagtga tgcagcaata atagtaatag
     6181 atgcaacagc gcctattcaa gttggtacag agaaatcatt agaactaact gagagtattc
     6241 ctaagataat gtttataaat aaaatagata atgaaaaggc aagatacaaa gatgcaatag
     6301 caatgttaag agaaaaatac aataataaga tagttcctat gataagtcct atatacaaag
     6361 ataaaaactt tgtaaagtta cataatgttt ttgaaaacat agatgattta gaaggtgaat
     6421 ttaaggaaca agctatgagt gttaaagaag cattaatgga acttatagca gaaactgatg
     6481 accaaatatt agataaatat tttaatggag aagaactaac tacggaagag atacaaaaag
     6541 gtattattat aggtatacaa agaggtgata ttatacctgt aatatgtggt tcaactataa
     6601 ataatatagg cacaaaggaa atattagata caatatcaag ttatttagaa cctatcttta
     6661 cagaggaaag caaaccattt aggggattag tatttaaaac tatggttgac ccatttgttg
     6721 gaaagatgtc atatataaag attactgagg gtgtactttc taaggataaa gatgttttta
     6781 atataaataa aaatgtaaaa gaaaaaatag ctaatatata tacattgaga aatagtgaat
     6841 tagtagaaat agaaaaagct aaagctggtg atatagtagt tataactaaa gttaattctc
     6901 taaaaacagg agacactata tctgcagata aagatgcaga agctttagaa aagatagatt
     6961 ttcctaaacc acaaatatat tatgcagtaa ctcctaagaa taaaggtgat gaagaaaaag
     7021 tagcatctgt attaaataaa ctagtagaag aggaccctac attacattgg tatagaaata
     7081 cagagacaaa acaagcactt ttaggaggtc aaggagaact tcatataaaa acaattaaaa
     7141 ataaaatgaa agataagttt ggtgttgatg ttgaattgaa tgaccttaaa gttccttata
     7201 gagaaactat aaaaggaaca gccgatgtac aaggaaaaca taaaaagcaa tcaggtggtc
     7261 atggtcaata tggagacgta aaaataagat ttgaaagatg tgaatctgat tttgaattta
     7321 cagaagaaat atttggtggt tctgtaccaa aacaatatat accagcagtt gaaaaaggtc
     7381 taaaagactc tatgcaaaaa ggtatattag caggttatcc tgttacaaac ataaaagcta
     7441 cgctttatga tggttcatac catgatgtag actcttctga aatggccttt aagatggcag
     7501 caagtgcagc ctttaaaaaa ggtatggaag aagcgcaccc aatactacta gaacctataa
     7561 tgaaacttaa gataactgtt ccagaagaat atatgggaga tgtcatgggt gatataaata
     7621 agagaagagg aaaaatattt ggaatggaac cagatgacaa agggaaacaa ataatatttg
     7681 cagaagctcc acaagcagag acatttaaat atgcaataga tttaagagca atgacacaag
     7741 gtagaggata ctttgaaatg gaattagaga gatatggaga ggttccatca cagtttgcag
     7801 aaaaaataat tgggttagct actgcaaagt aatgaacaaa ataaatagaa aaataattaa
     7861 tatatattat taaaaatatt tttagggttt taactaggta taggtttatg ttaggtaaac
     7921 ttatacctag ttttta
//
LOCUS       DAELSY010000164         7904 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.164, whole genome shotgun sequence.
ACCESSION   DAELSY010000164 DAELSY010000000
VERSION     DAELSY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7904
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.164"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(155..1183)
                     /locus_tag="KPG09_003278"
     CDS             complement(155..1183)
                     /locus_tag="KPG09_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG0967842.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDINKKMDTIDKLKDI
                     EEGYFYLLEDVYLDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NLNVVNKI"
     gene            complement(1231..2091)
                     /locus_tag="KPG09_003279"
     CDS             complement(1231..2091)
                     /locus_tag="KPG09_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBG0967843.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKSI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2370..2579
                     /locus_tag="KPG09_003280"
     CDS             2370..2579
                     /locus_tag="KPG09_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG0967844.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2579..2845
                     /locus_tag="KPG09_003281"
     CDS             2579..2845
                     /locus_tag="KPG09_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG0967845.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2874..3446
                     /locus_tag="KPG09_003282"
     CDS             2874..3446
                     /locus_tag="KPG09_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG0967846.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3680..4870)
                     /locus_tag="KPG09_003283"
     CDS             complement(3680..4870)
                     /locus_tag="KPG09_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG0967847.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENVENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSAGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(5130..5561)
                     /gene="dut"
                     /locus_tag="KPG09_003284"
     CDS             complement(5130..5561)
                     /gene="dut"
                     /locus_tag="KPG09_003284"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBG0967848.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5674..7902
                     /locus_tag="KPG09_003285"
     CDS             5674..7902
                     /locus_tag="KPG09_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBG0967849.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEYEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
BASE COUNT         2762 a         1248 c          989 g         2905 t
ORIGIN      
        1 acctaaagca atatacaata aaaaaatgta gaaagtaagt atctctctac attttttatt
       61 atatattatt tttatattaa tttaatttta tcttgattca gttttataaa aaattgttat
      121 aattgtcaag ttctactcta ttatttgtaa tttcttatat cttgttaaca acatttaagt
      181 tattacattt atttatatat attttttcat tatctaagtc ttcatcaaac atttttatta
      241 tattttttat ctcatacttg tactcatctt cattcattct ataaagcatt aaatcttcta
      301 atacatcttt catgtcgagg tacacatctt ctaataaata aaaatagcct tcctctatat
      361 ctttcaattt atctatagta tccatttttt tatttatatc tttcataatt tctaaatttt
      421 gtatatttac atctatgatt gttaataagt acattctcac agtttttatt atttcaacag
      481 aaaaatctac aatattataa tttccttctt ttttatataa tgactcctta aatttgttaa
      541 aacaattatc taaattagaa tttatatcgt taaatggtgg tcttattatg tcacgtatta
      601 ttaaaagatt ttgtatatct acattatttg tgattgcctc atccaaaata aataaattat
      661 atgcatcatt taatgggtca tgggcatctc cactaaactc taagttaaca aattcaagta
      721 aatttttcaa tgatatatag ttcatacatc tcataccaca atttatatat ttttctttta
      781 tatctacaaa taaatctcta atattattta caaaccttgg atggttattt tttttagcac
      841 ttctcatatc tgtgttattg aaacatgtca aatccaaatt accaaaagta tatattcctt
      901 taatatcaga aaatatgcca agccattttt taaattttct catgacctct tcataacttg
      961 gtgcactctt caattcttca gaagatatat gtgttaattc ttgtatatga ggatataact
     1021 cctcattact tactggtctt attaaagatt taaatttatc aatatttttt gttttttcat
     1081 catacataac tgctccgatt gcaattatat cagaattaaa tactgctctt tttgatttag
     1141 aatttggcat attcatttca aaatctatat atactctttt caatataaac accctttcca
     1201 attgtttata gtgattcttt ttaactattt ttattcaaaa ttttctaaga aagaatgttt
     1261 ctccccatct ttttgccaac caagtactga atcaagattt acattttctg ctgccttaaa
     1321 tatagatttt tctacatttt taaaatttga ttctaagctc tttattaagt cttttatctc
     1381 atatctttta ttacctgttt ttttagcagc aaccatacat gcacaattta atggccatat
     1441 accactattt tgaataaatc ttataatatg ttcttctctt atataataaa gtggtcttat
     1501 aatttttata ccttcaaaat tagtagaatt aagtttagga agcattgttt taaaatttcc
     1561 agcacacaat aagttaagca ttgttgtctc tattacatca tcataatgat gacctaatgc
     1621 aagtttgtta caccctaatt cttctgcctt tgaatataat gctcctctcc tcattcttgc
     1681 acacatatag catggataat cttttgctat ttcatctgct atctcaaata ttcttgaatc
     1741 aaataaatgt attggtatat ttagatactc acaattatct ataagtaatt gtcttatatt
     1801 tgcatggtat ccagggtcca tagcaataaa ttcaacatca aaattaactt gtccatgttt
     1861 ttttaactct tgaaacattt tagccataag aatactgtct tttccaccag aaatcgctac
     1921 agctatctta tccccttctt caaccaattt ataatctctt attgacttca taaacttaga
     1981 ccataaattt tttctatatt tctttattat acttttttct atatctctta ctggaagtct
     2041 ttcctcaaaa ggaactataa tttcacagcc ttttcctgat agttcactca tctaatcacc
     2101 tcgtatattt atgtatattt atgttaataa tattttacaa aaaagtctac cttcatccac
     2161 aaaatactat aatttttact ttaattttac taaataattt atagtttaca ataacacata
     2221 aacttataat aacataaata acctctttct tttattatct tttttatttt tctcatttaa
     2281 tattttatta ttagtaacga tttttaaatg attgaatatt atacactaga tagagttacc
     2341 tatctattta caacaaggag gttttcatct tggaaaataa gactagagat atcaaaaaaa
     2401 attgtggtcc atctttggct agaccataca ttaataatca aattttcaca gaaatgtact
     2461 gcctttctga tgcattaaaa tttggaacta tatttccaga actaaactta ctagagtctg
     2521 aaatgtacaa caaagaatta tatgctaagc caaaaaaata tagaggaggt agaagataat
     2581 gaaagactca tctagagctg aacttatgag aaaagttcag gaaacatctt ttgcatgtgt
     2641 tgatatgaac ttatatttag acaatcaccc tgatgataaa aatgctatca acacttataa
     2701 ttcattatgt aatcaatttg cgcaagctag atatgcttac gaaaataaat acggtccttt
     2761 aactaacttt gggtatgctc caagtagatg cccttggcaa tgggctgacc aaccttggcc
     2821 ttgggacaga gaatttaatt attagacatc aagtattgga ggattgaaaa ttcatgtgga
     2881 tatatcagaa aactatacaa cacccagtta atataaaaac ttgtgaccct agaatggcta
     2941 aatttcttat aactcaattt ggtgggccaa atggagaact tgctgcatct ttaagatatt
     3001 taagccaaag atatacaatg cctactggta atatgcgtgc acttttaaca gatattggta
     3061 cagaagagct agctcacgtt gagcttatat gtactatggt ttatcaatta acttctgatg
     3121 caagcccaga agagttaaaa gctgcaggtc ttggttcaaa ctatgctcaa aatggatatg
     3181 gaatttatcc aacagattca aatggtgttc catttgatgt aagacctata gcagttatgt
     3241 caaatcccgt aaccgactta catgaggata tggcagctga acaaaaagca cttgcaactt
     3301 attatcaact tataaaccta acagatgacg ttgatgttat agatgtatta aaattcttag
     3361 gtcaaagaga aataattcac tatcaaagat ttggtgaagc tttaatggat gcttacgagt
     3421 tagaagaatc tcaaaaaatg ttctaaaatt tttatttgta taactattat tatttgataa
     3481 tttataattt actgttgtgt taaaatttat aaaaaatata aatataattt attttctata
     3541 aacagagaaa ataaataaaa agcaggtatc aatcaaatag taacatgtat tatactatac
     3601 attactatca atattgtacc tgctttcata ttataagcaa taaagaaaaa tctatttatt
     3661 aggttttata ataaaacaat tataaaactc ttcttgcatt tacatatcta cttgaatagt
     3721 agctactatt tatattagat attgttactt tttttgatga acctgaagat gcatgtatca
     3781 tatctccatt tcctatgtat attccaacat gacttacaga accattattt gaaccttgtg
     3841 tatcaaagaa tactaaatct ccacttctaa ggtctcctct atttacatat gttccatact
     3901 tactttggtc tctagatact cttggtatac taactcctgc agattttttc ataacgtact
     3961 gtgtgaatcc agaacagtca aaactatttg gaccttctgc tccccataca tatggttttc
     4021 caagtagtgt ctttgcaaaa cttattacac tatcagctcc ttgacttgga gaattagtcg
     4081 aataagatgg aattgaagta tcaacagaac ctttttcagc aaggtattga cttgacacat
     4141 atccagtttg attgccataa cttacttttg tccatcctcc actttcagaa atacttccaa
     4201 cctcagtacc ataacttaat gtagctattt tagaatgact tgtacctggt cctgttctaa
     4261 cgttcaatga ctttgctgtt acaacctttg taccctttac aaggtcattt gaactgtttt
     4321 caacattttc atttgtagag tcatttacac tagttcctaa atatttactt gaaacataac
     4381 ctactctacc attagaactt atttttgacc atccatttga ctcagatatg tatccgactt
     4441 cagtcccata tcctagtgtt gatattttag aactacttgt acttggtcct gttctaaaat
     4501 ttaatccttt tgctgttaca acttttgtcg acttaactga agtatcacta ctttcattgc
     4561 tacttccaag gctatttatt gctacataat ttccatgcac ataacctgtg tttccattat
     4621 atttaacttt tacccaagaa ccttcttttc ctacgtactc aactttatca cctttgtaaa
     4681 gtttccccat tgattcatgg tttgtacctg gtccttttct aaaatttacc gcattccctg
     4741 ttattgttct gctttctata ctcacactct ctacttcaga ctcattagca tatgctaaat
     4801 tccccatagt aggcattatt atcgcagttg ccattatact ggctgctatt ctcttttgat
     4861 taaaattcat aactttccac tcatctcctc ttttctacta tctttatttc acttcaaaat
     4921 ataaaagaaa taattgacac tattaaaata tcaattattt cttttatatt aatattatta
     4981 caaatttgta actatttgtc tatagtaata tttgcttacc ataaattacc atgtataaat
     5041 tttaatcttc aatagcctgt aatttagtaa tatagccatc ttttaccttt tttaaaaatg
     5101 gctatatttt ttaacttttt tgtaactttt taaaatccag ttgagccaaa cccacctttt
     5161 cctctttcgg agtcacttaa ttcttttact tcttctatgt taatatcata gataggtttt
     5221 actatcattt gagcaatttt catatgcttt tcaaccttaa aatcattttt cccatgattt
     5281 attaatataa ttttaagctc tcctctgtat ccttcgtcaa tagttcctgg tgtatttaat
     5341 acagtaacag aatgctttaa ggcaagccca cttctaggtc ttacctgcgc ttctgtcata
     5401 gtaggaagct ctatacatat cccagttttg ataagtttag tctctccagg aggtattaca
     5461 acctcttcta tagaatacaa atccatacca gcatcacctt tgtgagcaaa attaggtatt
     5521 atagcatcat catttaattt ttttactttt aaattgtaca tcttatatct cctttcaact
     5581 ttctataaat attataactt aaatttcgac ctcttgaagt tttttatttt tttgtggtaa
     5641 tataattatt agtgttttaa aggaggattt tttatgaaac ttgtacttgc tgagaaacca
     5701 tctgtggcaa aaactatagc atcattttta ggtgcaaaga ccagacaaga tggttacttt
     5761 gaaggaaatg attatataat tacttatgca gttggacact tagtatcatt atatgatatg
     5821 aaggattatg ataaagataa atactctggc tcttggaaaa tgaacaattt cccatttgtt
     5881 cctgaggata agtttaaatt taagattgat agttctaaaa ctaaacaatt caacactgta
     5941 aaaaagcttt tgaatagaaa agatgtagaa tatgtaataa atgccactga caatgaccga
     6001 gaaggagaac ttatagcatt tttaatattt ttactggcaa aaaataaaaa accagtaaaa
     6061 agaatcttgg taaatgaatg gacaccagaa gatattacaa gaggtataaa aaacctaaaa
     6121 gatgaatatg aaatgagaaa tcttcaagct gctggatata ctaggcttat tactgactgg
     6181 ttgattggaa taaatttcac ttctgttgct acattgaaat atggaaatgg gaaactatta
     6241 aacataggta gggtaattct tccaacagtt aaattagtct atgatagaga tatggaaata
     6301 ctaaactttg ttcctaaaac ttattatgaa atagaaggac attttaaagc tgaagctggt
     6361 gaatataaag gtaaatatgt taagggtaaa gaaagtaaat ttgatacact tgaagatgca
     6421 aataaaatta tagcttcaat tacatctgaa actggtaaaa tactagataa aaaagttact
     6481 atgtcaaagg aatatgcacc aaaacttttt agtttgacat cacttcaagg atatataact
     6541 tctaagtatt ccaattttac atctgataag gttttaaatg tctgtcaatc tctctatgag
     6601 ggtaatggta agggtggata cataacttac cctagaactg attccatttt cttagaagaa
     6661 agtttggctt caaaagcttc tcaaacttta gataaattaa aggttggtct tgaatatgaa
     6721 aataaaatta aattttctaa aactaaaaga gtttttgatt cttctaaagt agactcccat
     6781 agtgctataa ttccaactta tataattcca aaaagtttat ctaaggatga acaattagtt
     6841 tatgatgcca taaaagatag atttgtagca aactttatgc ctcctgctga atatgagaat
     6901 acagaaatta agactgaagt agataattgt actttcttaa caaaaggtaa agtgcttaaa
     6961 tcaaaaggat accttgaggt atataataag gaagaaaaaa acgatttact tccacttgta
     7021 aataaagatg atgttgtaga tgtgttagaa ataaaaccat taactaagca aactactcca
     7081 cctaaaccat atactgagga caccctttta aaggctatga aaaattgtgg taaaaatgtg
     7141 cctgaagaag atacaacagt gctatctgga tattcgattg gaacatcagc cacacgtgct
     7201 gatgtgttaa agaagataag tcaagtgggc tatgtaaaga aaaaagggaa atcttattct
     7261 ataacagacc ttgggaaaaa tttagttgaa atttttcctg ttaaagactt atttgatgta
     7321 gattatactg gaaaacttga aaaaagttta agcgatattc aaaaaggaaa gtatacaaga
     7381 aaagaatatc ttactaatat cattagtttt atatatcaaa atgtcaatat tataaaacaa
     7441 gatgcttcaa aaaatataaa cacagaagca tatactttcg attcaaaaac taaaaaattt
     7501 actaaaacaa aagaaaaaaa acagaaaaaa gatgtaacta atactgaaaa agaagttgct
     7561 atatcaaaag attctaataa caaaaaagaa gttaaaaatg gtgatgaaaa tcaatctctt
     7621 ggcaaatgcc ccatttgtca aggtgatgta ctagaatttg acaaaggctt tgcttgtaaa
     7681 aatcataaag aatgtaaatt tgttatttgg aaaaatgata agtatttaag tctttataaa
     7741 aagaaagtta acaaaacaat ggtcaaaaat attttaaaaa aaggtgaaac taaagtaaaa
     7801 tctttaactg ctaaaaacgg taataaattt gatgctacat taaaatatga taaaaatcca
     7861 gataccggct atttcaattg gaaaatagag tttgacaact aaat
//
LOCUS       DAELSY010000165         7874 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.165, whole genome shotgun sequence.
ACCESSION   DAELSY010000165 DAELSY010000000
VERSION     DAELSY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7874
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.165"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(68..1345)
                     /gene="hflX"
                     /locus_tag="KPG09_003286"
     CDS             complement(68..1345)
                     /gene="hflX"
                     /locus_tag="KPG09_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG0967850.1"
                     /translation="MDMTKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELYDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDCVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            complement(2064..3896)
                     /locus_tag="KPG09_003287"
     CDS             complement(2064..3896)
                     /locus_tag="KPG09_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967851.1"
                     /translation="MSRMGRGPMGKSMEAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(3889..6135)
                     /locus_tag="KPG09_003288"
     CDS             complement(3889..6135)
                     /locus_tag="KPG09_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967852.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(6203..6664)
                     /locus_tag="KPG09_003289"
     CDS             complement(6203..6664)
                     /locus_tag="KPG09_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0967853.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(6969..7535)
                     /locus_tag="KPG09_003290"
     CDS             complement(6969..7535)
                     /locus_tag="KPG09_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBG0967854.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         2548 a         1407 c          789 g         3130 t
ORIGIN      
        1 atttacaatc aatttataat aaattataaa ttaaatatat tttaggctat aataagtttt
       61 tcaaaattta ctctaaagca tactttttat acttattata ttctatattg ctacattcta
      121 catctaaaat agctccatct tctctgtatt ctgtatttat aatgctcgta ttttcgttaa
      181 aataagaaac tacatgacct ttatcatatg gaattaagaa cttacatctt acacaatctt
      241 taaatatgct atcacaaata cattttatca ttttatctat atttatatct tttttaactg
      301 aaacaaatat accttcttta tcgatactat ctaaaattct atccaatact tctacatcta
      361 ttaaatcaat tttattatac acatgtatca taggtatacc atctgcacct atttgtttta
      421 aagtatcttc tgtcacttta atatgacttt tatagcttgg atttgaaata tcaataacat
      481 gtaacagtac gtctgcctca catgcttctt ctaatgttga cctaaatgct tttactaagt
      541 catgtggtag attacttaca aaaccaacag tatcagataa taaaaattct ttgttatttg
      601 caagaactat atttctgaca gaagtttcaa gtgtagcaaa taacatgttt ttctcaaaca
      661 ctttcttttc ttcagagttt ttaaatatat ctactagttt attcattatt gaagatttac
      721 cagcatttgt atacccgact agagctacat ttggaagatt tgattttctt ctcatacttc
      781 tttgagtttc tctttggaat tttaaatcat acaactccct atttaaagat gttatctttt
      841 cttctattct tctacggtca agttctaatt ttttctcacc agaacctcta tttttagtac
      901 caactccacc actttgacgt cctaaatttt catttgaacc tataagtctt ggtaacatat
      961 attttaaact tgctacttct acttgaagtt tagcttctct agtttttgcc ctttgtgcaa
     1021 atatatcaag tatcaatgcc gttctatcta taatttcaca attaagcttt tcttcaagat
     1081 tttttagttg tgatgtagaa agttcattgt aaaatataac tatctctatg ttttctttct
     1141 ttattaagtt taatatttcc tcaactttac ctgtcccaat gtaaaatgct ctattaacct
     1201 gctttgcatt ttgagttata ctccctacaa catcaatatc acatgctgaa cataagtttt
     1261 caagttcaat cattagttca ttaaagtcct cagatttatt atttatgttt aatcctactg
     1321 ttattccttt tttcgtcata tccatattct ccttatctgc tattttttat ttaatctata
     1381 gcacgagatt atggctctat aattaaattc atcaagaatt ttgcttttta tcttatcctt
     1441 aaatgacact tcttatttac tattataata accataatcg tgcattattt tctcaatctt
     1501 atataaaaaa tcactttctt acacctcctc ccaaatatat tatgcagttt tttcaataaa
     1561 aaaactcctt acaaatctaa gtaatacaaa acaagctact tatatcataa gaaggattaa
     1621 aattctaaat atatattaaa aatacattag tttaattaac ttatatgtaa ttatcatttt
     1681 atatttaacc ctattcttaa atttaagtag ttatttgcat gattaataaa agtaataaaa
     1741 acatatactt caatataatc atcaactatt aaaattaaat tctatttata ataaaattta
     1801 aagaataaaa ttaaatatac attttgataa tcacctccaa cattttcctt actacatatt
     1861 ataataaaat aaacactttg taaagacata aaattatgtg gctatctcaa aatatagata
     1921 atcctctttt tcattgttaa aatgataaaa atacacttta tttcatacac aaaagagtgc
     1981 cttatgaatt aaaaagttca ttttaaggca ccctcattta ttttatctta ttaattacat
     2041 gtctatgcta agatagtttt ttgttacatt gcttcatctt cttcaaattg agaattgtaa
     2101 agattggcat agaatccgcc tttctttaga agttcctcat gatttccttg ttcaactata
     2161 tctccttcat tcattacaag aatcatatca gcatcacgta tagttgataa tctgtgggct
     2221 ataacaaaac ttgttctacc ttccataaga ttatccatag ctttttgaat caatacttct
     2281 gttctagtat cgacagaact tgtagcttca tctaatatta aaattttagg atcagctaaa
     2341 atagcacgag ctatagtcaa taattgtttt tgaccttgag aaacattact tgcttcttca
     2401 tttaatttca tcttatatcc atctggtaac gtcttaataa atctatgaac atgtgcagat
     2461 ttagcagcct ctataacttc ttcatctgta gcattcaact taccatatct tatattttcc
     2521 ataattgaac cactaaataa ccatgtatct tgaagaacca taccaaacat ttctctaagc
     2581 tcactacgat taaaatcttt aatattatga ccatcaatca gtattgaacc gctattaaca
     2641 tcataaaatc tcattaacag tttaactatt gtagttttac ctgctcctgt aggtccaaca
     2701 atagcaactt tttgaccagg tttaacattt actgagaaat catttattat agtcttgttt
     2761 gggttatatc caaagttgac attttcaaaa tcaattttac cttctaaacc atctattgaa
     2821 actgcatttt caacaatttg gacttcttct tcctcttcta aaaactcaaa tactctttca
     2881 gaagctgctg ctgttgattg caataaattg gcaacctgtg caatctgagt cattggctgt
     2941 gtaaagtttc ttacatattg tataaatgac tgtatgtcac caacctcaat tgttttctta
     3001 attgcaagcc aacctcctaa gattgatacc attacatatc ccaagtttcc aataaacatc
     3061 ataagtggtt gcattatacc tgataaaaat tgagatttcc acgctgaatt atacaggcta
     3121 tcatttagtt tatcaaactc ttcaattact tcatcttcac gattaaatgc tttcactatt
     3181 gtttggccac tataagtttc ctcaacttga ccatttacat ttcctaaata ttcttgttga
     3241 tatctaaagt atttttgtga tttctttatt attcttgaaa ttacaaacat tgatattggt
     3301 aatattaaaa ctgccactaa tgtcataatt ccacttatag ataacatcat tatcaaaaca
     3361 cctatcatag ttgttactga tgttataagc tgtgtcatac tttgatttaa actttgattt
     3421 aaagtatcta tatcattcgt aattctcgat aatatttcac catgcgtctt agtatcaaaa
     3481 tacttcattg gtaatctatc caattttgct gaaatctctt ttcttaaatt ataggatact
     3541 ttttgagaaa ttccactcat tataaatcct tgtataaaag agaacagtgc acttatcaaa
     3601 taaaggaaaa gtaaaaaggt taagattttt ccaattgcat taaaatctat acctacgttt
     3661 cctccagaaa ctttactcac taacccttca aatatctttg tagttgcctt tcctaaaatt
     3721 tttggaccta ctatagaaaa cgatgcacta ccaatagcaa atacaattac taaaattatt
     3781 gatattttaa atttagataa atatgcaatt agttttcgca tagtcccttt aaagtcattt
     3841 gctttttgtc ctgcttccat agattttccc ataggacctc ttcccattct actcattagc
     3901 tagctcctcc tttgaaagtt gtgataaagc tatttgctca taaacttcgc aattttttag
     3961 aagctcttta tgtgttccta ttccaactat ttctccctca tttaaaacaa ttatctgttc
     4021 tgcatttaag atagtactaa ttctttgtgc aactattaga actgtacttt cagcagtttt
     4081 tgattttaaa gccttacgaa gagctgcatc agtttttaag tcaagtgctg aaaaactatc
     4141 atcaaatata taaacttctg gattttttgc tattgctctt gcaatagaaa gtctttgttt
     4201 ttgacctcca gatacatttg ttccaccttg agaaatttca gttttaaaag cttctggttt
     4261 agaatctata aattctgtag cttgagctat ttcagctgct gttctcattt cttcatctgt
     4321 tgcgtctttt ctaccatatt ttaagttgga ttctatagta ccagaaaaca atactccctt
     4381 ttgaggtaca tatccaattt tttcccttaa atcatgttga gaaacatttt ttatattaac
     4441 tccaccaagt aatatttctc cttcagtaac atcaaagaaa cgaggaatta aatttataag
     4501 agttgactta ccactaccag tacttcctat aaaggcagtt gtttctcctg gctttgctgt
     4561 aaaagatata tttgttaata taggttcttc tgcgtctgga tacatgaacg aaacattttt
     4621 aaattcaaca agaccctttt tatcttcctt aaactcttgt gcctctgatt tttctgggtc
     4681 ttttatgctt atatctgaca ctaatacttc atctatacgt gtagctgaaa cggatgctct
     4741 tggaagcata atagatacca ttgatatcat taagaatgac attattattt gcattgtata
     4801 ttgtataaat gccatcatat ctccaacttg cattgctcca gaatcaacac tgtatgcacc
     4861 tctccaaact ataagaactg tgataaggtt cattataagc atcatagcag gcatcataca
     4921 tgtcataact ctatttacaa aaagatttgt tttagttaaa tttttatttg ctttatcaaa
     4981 tctttcttct tcatgttttt cagtactaaa agctctgata actggaatac cagtaattat
     5041 ttcacggctt actaagttta acttatcaac aagcttttga accagtttaa acttaggcat
     5101 aaccactgag aataacacta caattaatga taagatagca actactgcta ctgctataat
     5161 ccaggccatt gaagtatttg tgtttaaaac ctttatcact ccacctatag ccattatagg
     5221 tgcatagaat actattctaa gcaacataac catcaacatt tgtatctgtt gaatatcatt
     5281 tgtactacga gtaattaatg aagctgtaga aaattcattc atttcagtat ttgaaaatga
     5341 cataactttt ctaaatactc cacttcttaa atttctacct acacttgctg ctactcttgc
     5401 tgctaaaaat cctactgtta ttgtagctac catactaatc attgctatac caagcatttt
     5461 agcaccagta aataatatat aattattttg agttttttct gtatcaatac ctattttctc
     5521 atattctgac cttacatatg aaacagagct ttgtgttatc atactctctg gcatactttc
     5581 aaagcctttg ctcatctctt tagtcaatgt atctaactgt tcctttggca ttgctgataa
     5641 caatttaaat atatcaacat ctccactttg agtagcttgt ggtggaagtt tactcattaa
     5701 ctgagctttc atagctttta cctctttact atcgccctca agccctgata ctattaaaat
     5761 agctttacca aaaataacat ttaaatcatc tatagtgtct ttgtctttag tatttaactc
     5821 ataaagagat tcatctttta gacctgggta cttatcttta tacttaacat aatccttttt
     5881 attcagcaaa gtataattat ctagtacttt atccttacta cttttatcca taaataaagt
     5941 aattttattt aattcactct ctctaatcac agatggaaca gagttttcta ctccaccttg
     6001 ttgtatacct acatttacta tattggatgt gtattctggt aaagaaaggt cacataccgc
     6061 ttgaattacc aatagacaga ctattattac tattgataat gccgatttct ttaaatattt
     6121 aattaattta aacatacatt tcttcccttc tgtaatatta attagtctaa taacattaaa
     6181 ttgaaaaaca ttattttaaa ctttaaaaat atttttcatc ttcatgctta tgacatcctg
     6241 cttttaaatt atctcgtact tgcattagaa gtcttctaag tactattttt tcttcttcac
     6301 taaatcctaa aaaaaactct ctttccaatt ccttgttaaa ttcttcaact ttattacata
     6361 tttctaaacc tttgtctgtc aaaaaaatcc tagatactct ttggtccttt tcatcttgtt
     6421 ttctttccac aagtccagct ttttccatcc tctttatcat aactgtagtt gtagatgctt
     6481 ttactttcct tttatctgct aattcttttt gactttgccc atctttcatc tttaaagaaa
     6541 aaagtatttg gggttgacca tgatataagc ctaatttatc aaaataaaaa tgagttctta
     6601 taaaatatat tcgcattaat tgcaagaaaa catcatttaa tttttcttca gaattgctat
     6661 tcatacttgc cctccttatt ttttatatta cttagtcggc taagcattat tttactatac
     6721 tgcatgcatt ttttcaatat atttataaaa aattcatata aatttcattt attaaatatt
     6781 tttatatcat atagttataa ttttaataaa taatagtaag atttttaatt agtcagctac
     6841 ttatattttt tattgtaaag cttaaaataa accttttcat atattttcaa ataaaaaagt
     6901 tgtcccaaaa taaattttga aacaacatca tattcaacag tatcttgatt atataacatt
     6961 acaatactct acttatcttc atatccattt acaacaacgt ctaacttccc agtttcaggt
     7021 gatataatta gaccatgtac aataatacca tctggaatta aaggatgccc ttttattaaa
     7081 tcaatactat cttttacaga ttcttctgca gaatcgaatc catgtaacca ttgttttgta
     7141 tcaattccag cactacgaag tgtaagtatt acatcattag atattcctct atctaaaatt
     7201 ttaccaagta acttatctgt atcaacatta cacataccac atccatgatg ccctacaacc
     7261 ataacctcat ctacatcaaa ttcgtaaatt gcaactaaaa cacttctcat aatacttcca
     7321 aaaggatgca ttatagtagc tcctgcattt tttattagtt tcacatctcc atttttcaaa
     7381 ttcatagcct taggcaaaag ctcagttaat cttgtatcca tacaagataa gactactatt
     7441 tttttatttg gatgttttga tgtcacatat tgttcatatt ctttgttctc tacaaatgat
     7501 ttattatatt ctaatatctc ttcaagtttt ctcatatgta atatcaccct ctttaaaaat
     7561 ttttttatta ttatatcact attaaagtac aatatataca catataattt aatttatatt
     7621 tattaaatta ttttataaga ttattactct actataaatc ttgtatataa cttgtattta
     7681 taagtatttg tatatttatt ttttataatc taaactaata ttatatattt tcattatttt
     7741 tttaattata atttttttaa aattttattc taaatgcctt acttataatt aattttttat
     7801 ttcacctata tataattaga tttaaaaatg tttaccttac caatataatg attaaactca
     7861 aaagtcttat tgaa
//
LOCUS       DAELSY010000166         7826 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.166, whole genome shotgun sequence.
ACCESSION   DAELSY010000166 DAELSY010000000
VERSION     DAELSY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7826
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.166"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            100..2001
                     /locus_tag="KPG09_003291"
     CDS             100..2001
                     /locus_tag="KPG09_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0967855.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASNLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            2004..3047
                     /gene="ypdE"
                     /locus_tag="KPG09_003292"
     CDS             2004..3047
                     /gene="ypdE"
                     /locus_tag="KPG09_003292"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG0967856.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            3062..3367
                     /locus_tag="KPG09_003293"
     CDS             3062..3367
                     /locus_tag="KPG09_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0967857.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPTMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            3464..3799
                     /locus_tag="KPG09_003294"
     CDS             3464..3799
                     /locus_tag="KPG09_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0967858.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            3831..5153
                     /locus_tag="KPG09_003295"
     CDS             3831..5153
                     /locus_tag="KPG09_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0967859.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            5163..6233
                     /locus_tag="KPG09_003296"
     CDS             5163..6233
                     /locus_tag="KPG09_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0967860.1"
                     /translation="MNNTIERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            6238..7425
                     /locus_tag="KPG09_003297"
     CDS             6238..7425
                     /locus_tag="KPG09_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0967861.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVANNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(7622..>7826)
                     /locus_tag="KPG09_003298"
     CDS             complement(7622..>7826)
                     /locus_tag="KPG09_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBG0967862.1"
                     /translation="SMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYK
                     DKKFINKLSKGKVFTASYNINIH"
BASE COUNT         3080 a          844 c         1420 g         2482 t
ORIGIN      
        1 atataaaatt cttaaataaa aagaaaaggt tgtttttata aaaaatattt tttataccac
       61 tttaaaaaac acaataataa cactgaggga ggtggggata tgagagaacg cattattctt
      121 gaaacattaa aaaatagtag tggttatcta aacatagatt attttgcaga taagttgggg
      181 gtatctactc gaactatcag aaatgaaata aaaaagatag aaactataaa agaatgtaat
      241 ggttttaaat tagaatataa gtctaaatta ggatatattc taaatattaa agaccaagat
      301 aaattcgagc attatctaag aaacttaccc tcagatttag ttgaaaatcc agaacaacgg
      361 ttggagtcta taatagtaga gcttttagta aatgaaggtt ataaaactat agaacagtta
      421 agtaaaaaat ttctagtaag ttcatcccaa ataaaaaatg atttaaaaaa gatagatgaa
      481 aagataaagg atacagaatt aaaactagag cgaaaggctc attatggaat taaaatagaa
      541 ggttctatta aagaaattca aagtatattg gtggatagtt attttagagg gaatagaaat
      601 ataacagagt acaggaataa gtttatagat aatttaaaat tagctaatat tagaagtaca
      661 ataaaaaatg tacttaatga acatgattta gaagcaaatt taacagaact tgaggaaata
      721 ttagcacaaa ttattatatt gtatataaga gttagtatga gagtagtggg aaatgctagc
      781 aatttaaaat tatatacaga agatttaatt atagataaat tattagataa aatttttaga
      841 gataaaaaat ataaccttaa ttatgatgaa aagtattatt taaaggagct tataaaacta
      901 aagactaaag ataagaaagc cactataaag aatattgata agaataaact tcaagatatt
      961 atgttcgaat tttttagaga gatagataaa aaatataact ctaatttttt agaagataaa
     1021 gagtttttta acttatttta cttacatatt gcatgtttaa tagaacgtat aaagaagaac
     1081 cacaagataa taaatccttt ttcagttaaa ataagtcaac agtatccaac tgtttttaat
     1141 ttatctattc aattttcaaa aatcatagaa aatgagtatc atataaaaat aagtcaagat
     1201 gaaatagggt ttatagctac tcacattgct gttccttttg aaaagagaga agaagctaat
     1261 tttaataaaa aatataaaat agcaataata tgctcttctg gtggaggtag tgcatttttg
     1321 ataaatttaa gacttaaaga aatttttcca aatgctgaga taagaaattt ttcattacta
     1381 gaagaaaaag ctgtaataga atttgcacca gatttgattt tttcaataac aaatctttta
     1441 tttgaaataa atgctccagt tatacttata aatgaaattt tagacgaatt ggattattta
     1501 gatattaaag aaagtgttag atttgcagac catataggaa atatatcaag cccaaaacaa
     1561 tatatattag gtctatttga taagaatcac tttaggtgta taaaagataa agctaagtat
     1621 aaggatatcc ttgatagtat gtcaaaatgt atagttgacg agggagcttg ttctccaacc
     1681 tatcccaaag atgtatggga aagagaaagc tatttgagta ctatttatac aaatggggtg
     1741 gctatacctc atccaatcga gatgacagga aacaaaaata taatatcagt agctttgata
     1801 caaactgata ttatttatga aaatagggtg cccaaaatta ttttcatgat ttcattaata
     1861 aagggaaatt tagaacttca taagcaaata tctaaatatt taagtaagat tatggcaaat
     1921 aaggatatgg tagacatgct aaataagtca caatcttatg aagagtttat gtataagtta
     1981 aaaatctatc tgggaggtta gacatgaata tagagttttt taagaggttg tcagaagcag
     2041 atggtatagc atctaatgaa gaagaggtta gacatgtttt attggaagaa ctaaaggctt
     2101 atagtgataa aattatctgt gatgggctag ggagtatcat cttttctaaa ataaaagatg
     2161 agagtgcacc aaacgtaatg atttgtgctc atatggatga agttggattt atggtgagaa
     2221 gtatagacaa acttggtatg attcacttaa taacaatagg aggagtaaaa cctcttgcac
     2281 agtttgtaca aaaagttaga attacaacaa aagaaggaaa gaaaatccct gctgtcataa
     2341 atgctacata taataatggt aaggctgaaa atatatatgc agatattgga gcatatacag
     2401 aagaagatgt atataaccta gggattaatg ttggggatat ggtaacatat acaacaagtt
     2461 ttgaggaatt tactttacca gaccgattag tgggaaaggc ttttgatgat aggataggat
     2521 gttttgtgat gggagaagtt ttaaaagaac ttaggaaaga aaatctaaat tgtaatattc
     2581 actttgcggc tacaagtagt gaagaggttg gaataagggg agcaaagaca tcaacacagt
     2641 taataaatcc agatatagta tttgtaatag atgtagcatg tgctaaaaat gagtttgtga
     2701 gagactatac aaatcagaaa caaattgata aaggtgtaat gctaatgcat agagatagga
     2761 ctctagtccc tagtagaaaa atgatagatt atgtaatgga aattgctgat aaaaataata
     2821 taccacttca acatgatatg tttgaatcag gtgggactga tggaggagaa gcacatctcg
     2881 taaatgaagg gaaaccttgt gtagtaactt gtgtaccagt tagatatggt cattgtgcat
     2941 tttcaatagt aagtaataaa gatttagaga acattataag attgtataca cagttaattt
     3001 taaattttga tgaagaacaa tataaacata taagaaattt tatctaaagg agaataagaa
     3061 aatgtcaaca gaagtacaaa taatggagct tatatcaact gcaggagaga gtaaatcgaa
     3121 agcttttgag gctttaaaaa aagtaaagtc aaaagatttt aaaggagcaa aattgttaat
     3181 agaggaggct cgtaaaatag atattgaggc tcacagaata cagacagaat taataactca
     3241 agatttaaat gaagatgttg aaagcccaac aatgagtctt ttaatggtac atgctcaaga
     3301 ccactatatg acttctcagc tagcaaaaga cttgatagag gctttgatat attgtctcga
     3361 taagtaattg taatgtagaa atagattatt tatgtaaaaa ttcttttaaa agtttagatg
     3421 cttttaagag ataataaaaa tacaaacttt aggaggaatt attatgaaaa tattattatg
     3481 ttgtgcaggt ggattttcta ctaatatgtt aatgcaaaat atgaaaaagg tggtaaaaga
     3541 gagtgctaaa ctcaatgaaa aagattttga gtttacagct ataccagcag attcaataga
     3601 aagtgaagtt gatgattggg atatagtact tataggtcct caaatagctc ataaaataga
     3661 cttcttaaaa ggaatattag agccaaaaaa taagcctttt gcagtaatag ataaagatgt
     3721 atatggaaaa atggatggag ctacagttat gaagttggca ttaataactt ataagaaaca
     3781 tcaattagct caaggataat aaaaataatg agggaaaagg gagattagat atgttttcaa
     3841 aatttgaagc atttatgaat agagtattta tgcctttagc tcataaggtt gacaatcaac
     3901 gtcaccttgg ttctataaag gctggtatgg ttgctatgac accattcaca atattaggaa
     3961 gtatatttgc aatacttcca gcacttccaa atatgcttgg agaaaataat ccaataagca
     4021 aattcatact taacaattca gcaatattgg atttaccagt aagtctttcc ataggtttaa
     4081 taggattata tgcttgtatg tgtattgctt ttaatctggg gaactactat aaattatata
     4141 taccaggttg tgttgcacta tcaacttttt cttttttatt cctagtagcc tcatttacac
     4201 cagatggagc tttagaaata ggaaatttag gagctaaagg gctatttaca ggtatgatag
     4261 cagggcttgg ttctgtggag ttatataatt tttgtaataa gaagaatatc acaataaaaa
     4321 tgccagaaaa tgtacctgat tttgtatcta ggtcttttga attaatacct actactataa
     4381 tagtttgtgg aacttttata gttgctcgtt ttatttcatt aaaagtattt ggagaattac
     4441 ctccacagat acttactcaa tttttatctc cattagtagg aagtatggat aacccatggt
     4501 cagttcttgc acttaacttt gctatatgta ctatattctt ttttggaata cattcatcag
     4561 tatttagtcc tataactaga cctataatga ttacatttat agctgaaaac atagcagcta
     4621 tgcaagctgg agaaacaata cctcatttct ttacagcagg agcttcaagt gcattctttg
     4681 gatttacagg atgtggaatt agtataggtt gtgtaattgc atgtatgtta tcaaaaaatg
     4741 cttcctataa gaagattgga cgtgtatcat tattccctgc attatttggg ataaatgaac
     4801 caatattatt tggagcaccg ataatattaa atccaataat gtttatacca catgtatttg
     4861 gtggagctat aataggtaca ctaccaatgt tttttatgca ctggggatgg ttagcaaagc
     4921 cagtatttga ccctccatat gtaggggtat tcttagaagg atttttaact aatggagatt
     4981 ggcattctat attagcaaat gcattgcaat tagtattagc agttttatta tattggccat
     5041 tctttaaaat aatggaacgt caacaaaata ataaaaaaca aaaagaagaa actaagaata
     5101 tattctctaa agaagaagaa gacttattgg atgatttagg attggatttc taatattttg
     5161 ttatgaataa tacaatagag agattagtat ccttaatcta taaaagaggt gttgacgctc
     5221 ttttgattaa agcaaaaaat actaaaagat atataggagc tttatcaggt agtggtgtat
     5281 atgtgcttgt tactaaggat aaaaattatc aaattctaga tggcagatat attgatgaag
     5341 cagataaaaa aacgactggt ttcataaaaa ggattgtatc tcaaggttct tatataccaa
     5401 ctataattga gttattaaaa gaattaaata taaaaaagtt agcaatagaa aaccaagcta
     5461 tgagtataca agagtatctt tcactaaaaa atgaaggttt tgaaatcaat ttaataacaa
     5521 atgaattatg gaagctaagg gctataaaat ctaaagaaga aattgaactt attaaaaagg
     5581 cttgtgagat tacagatgaa gtatttgatg aggttatttc tgaaattaaa aaagatatga
     5641 ctgagctaga agtatcagct ctaattcagt atcatgcatt aaaaaaaggt gcaagtggaa
     5701 tgtcttttga aacaatagtg gtatcaggag aaagaggtgc aatgccacat ggaagaccta
     5761 caaacaaaaa attgaaagtg aatgaagcta taacaataga ttttggtgtt gtttatcaag
     5821 ggtatcaatc agacatgact cgtacaatat ctataggtaa acctcctaaa attattaaag
     5881 agatatatga tgtagtattg gaagcacaat tatctgcaat agagtcaata aaggaaggaa
     5941 ctagggcaag tgatgttgac aaagtggcta gaaaaattat agataaacat ggatttggcg
     6001 aatattttaa tcatggactg ggtcatggta taggtcttgg agatggtgaa gttcccactt
     6061 taaatccaaa tagtgaagat atattagtgg aaggaatggt aatgtcttgt gagccaggta
     6121 tttatattcc aaatgtaggg ggagttagaa tagaagatga tattgttata atagatggaa
     6181 aaggtgttcc actcaataag acatccaaag agtttataat attaggagag taaaattatg
     6241 aaatataatt ttaataaagt attaaatcgt aaagaaggaa attgtagaaa atggagcaac
     6301 catgtgatta aagaaaaatt cggcttaaat gaagatgcaa ttcctatgga tttagctgat
     6361 atcgattttg agtgtgctcc tgctataaaa gaatctatct taaaaagagc atcagtagga
     6421 gattatagtt atacttttat aggagatgac ttctatgatg ctattataaa ttggaataaa
     6481 agaagatttg atgtatatat agaaaaggat tggataaaat taacttttgg tactgtttca
     6541 acccttcatt atatagttca agcatatact aaagaaggag agggtgtatt gataaataca
     6601 cctgcttatg acccttttgc agaggctgta gcaaataata atagaaagtt gtgttgtagc
     6661 tcattaaaat tagataacaa tagatattat ttagatttag aggatatgga aaatcaaatg
     6721 aaatacgaga atataagatt acttatattc tgtagtccac aaaatccatc aggtcgtata
     6781 tggacaaaag aagaactata tcaagtgtca gagttatgtt taaagtataa tgtaattttg
     6841 gtaagtgatg aaattcatag agatataata tttaaagatt atagatttgt atctttgtgg
     6901 aatgctcatc cagaaattta taaaaatagt ataatctgcg tatctcctaa taaaggcttc
     6961 aacttaggtg gattaaaaac atcatatgta ttaataagag atgtaaaaat tagggagact
     7021 ttattagaaa ggctaaaaag taattcaata acttctccaa atgtatttgc aataccagca
     7081 atagttgcag catacaatga aagcgaagaa tggctagatg ctatgactag ttatgtagag
     7141 gagaattttg aaatagttta tgatttcttt gaaactaata taccaaaagc caaagtgatg
     7201 aagtcagact cttctttttt agcctggata gatgtaagag aagtatttag aaatgaagaa
     7261 gaatctaaag aattttttag acatgcaaac ttaactatgg tagttggaag ttattttgtg
     7321 aaagatggag atggatttat tcgtattaat gtggggtgtc ctagagaaac attaaatgag
     7381 gctctaaata gaatcaaaaa aacgtatata agtatgtatt gttaaatcat aaaaaaagta
     7441 tctatggttt atataaaaac acattattta gttatcaaat attactggaa ttttgtattc
     7501 cagtaatatt ttttgattaa ggaatattat tgaggaatat taaataatat tatttcatac
     7561 ccttatatta acttatataa aaatatttta ttattttggg aacgggttag ctaaccttta
     7621 tctagtgtat atttatattg taagaagcag taaaaacttt tcccttacta agtttattaa
     7681 taaacttctt atctttataa attttgttag aatttatact atcagcaaga ccataccaag
     7741 gttctataca taaaaaagga gcagtagaat tagtttcact ataataagga gtccaaatac
     7801 caactagagg gaaatcttcc atagat
//
LOCUS       DAELSY010000167         7767 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.167, whole genome shotgun sequence.
ACCESSION   DAELSY010000167 DAELSY010000000
VERSION     DAELSY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.167"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(396..2675)
                     /locus_tag="KPG09_003299"
     CDS             complement(396..2675)
                     /locus_tag="KPG09_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0967863.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITEKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYIARSNGNIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFAQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2946..4400)
                     /locus_tag="KPG09_003300"
     CDS             complement(2946..4400)
                     /locus_tag="KPG09_003300"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBG0967864.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYNSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4564..5433
                     /locus_tag="KPG09_003301"
     CDS             4564..5433
                     /locus_tag="KPG09_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0967865.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDATSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGYLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(5647..7719)
                     /locus_tag="KPG09_003302"
     CDS             complement(5647..7719)
                     /locus_tag="KPG09_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0967866.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLSHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYASIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKLLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSKKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNCLSTISPVVFTNLEQNQKLSIKKGITTAKEENRVSIL
                     RYDIEKIG"
BASE COUNT         2643 a         1282 c          821 g         3021 t
ORIGIN      
        1 attatataga atttaaaaat aaaagaagag tatttcaaat gatttgtata aattattatg
       61 aaaaactctt tttttgatat aataaaatta cttacatata taacatttaa atttataaat
      121 tatcttttta ttttttaaaa tatgtataac acacctatag aaattactat tattactatc
      181 taaagtattt tgctataatc ccatatactc ttacctcaaa aacattcaaa tggaaaaaat
      241 actacatatt aataaacttt gcccaataat tcctaaaata atatcctaac aatgatttat
      301 gtttttataa tagtatattt ttacataaaa aagtgaacat tgattaacaa agttcacttt
      361 tttataaatt tttatgttat aattcacaat tttatttaat ttgtttcttt tctatttaaa
      421 ctatatctat ctaatattaa tttttcatat tcttctattg gcattggtct agcaaataaa
      481 aatccttggg ccatatcaca acctataaac cttaaaaact ctgcttgctc tacagtttca
      541 acaccttctg aaacaacttt tatatcaagt tgctttgcca tttgaacaat acttgaaact
      601 acaatttgac tcttttttgc atttcccact gttataaaga attgcctatc taatttcaat
      661 acatcaattg gcatatcttt aagcatattt aaagaagaat aaccagaacc aaaatcatcc
      721 atagaaattt taaatccaac agatttcaaa gtttgcatta ttttaaataa tatttttgta
      781 ttgtcaaata ttgcactttc tgtcaattca atctcaatta aatttggttc aatttcatat
      841 tcttttgtta ttctttcaag ctcagaaata aaacctggat tctcaagatg tactcttgat
      901 aagtttattg atataggaaa tgtggggaaa ttttcatttt cccaacgttt aaaattttta
      961 caaacttggg caaatacaaa catatccaat tctgttataa aaccattttt ctcaaaaata
     1021 ggaataaata cacctggact tattagacct tttttgggat gttgccaacg tacaagcgct
     1081 tcagcaccta caattttagt agttgataaa tcaaccttag gctgtaaata aactttaaat
     1141 tgttcatttt ttaaagcatc atgcatttct tgttcaatat cacgttcttc tatgagagtt
     1201 tcttttaaat tttcagcata aatctcataa acttttaaat attttctttt tactgtagtt
     1261 tttgcaatca tagctttgtc aagacaggta gaaatatttg tttctccctc ttcaacaaaa
     1321 tatacaccta tggatggaat taaattgagt tctactccca aacttgtttt tatcattgac
     1381 aattttttac gtataagttc acatatttct ataattgact ctctattatt tttcttttca
     1441 aatattatag caaaattgtc atttgaaatt cttgaaaata cagattgctc tttaaatata
     1501 ttcttaatta tctctgctat ttcctttaat actctatctc cttcttcata cccaaaccta
     1561 tcatttacta gcttaaactt atctatatca aaataaatta aaactagatt ctttttctca
     1621 tgagaggata aaaattttcc tccttcaaga ttaaatttat ttgaattgcc aatacctgta
     1681 acgttatctt taaaagcaaa ttcttccaat ttttccttac tctttttatg tataaatagt
     1741 atataaattg ttaatgccgt aaagattata atcaacacta cacatgctga taatgtaagt
     1801 tttataatag cgtatgattt ctcagatata gcagttttag gaacttcaga aaaaagatac
     1861 caatcattga ttcctattgg ggcataactt aaataatatc cttttccaaa catactataa
     1921 cttaatgaac ctgatttaaa gttttttata tttgacttca tattattgat actagatttt
     1981 gatattgaat cacttctgcc aatgtaagaa aataaatttt ctttagtaga gttggcgttt
     2041 ttgctgcttg gatttaaaac aatattacca ttacttctag ctatataaga atatcctttt
     2101 ccattaaatg taggtacaga aagtattttt tcatacaact tcgtactatg tgtggcaaat
     2161 ataactcctt taacctcatt atctttataa attggcacag aatatacatt aatattacta
     2221 ttggtatttt tatcatcttc tattacatct tttaattttt ctgttataac cacttctcct
     2281 tgcattgatt ttaagaaata atctctttta gagaagtctt ctatataacc atctgtagtg
     2341 tatgctgtac catttggtaa aataactccc attcttttaa atgaactatt aatagcttgt
     2401 ctttttagta tagaaaggat attatctact tcaaaagact cttctccctc aataaatatt
     2461 gaaattgact ctagtaactt tatatctcca ttaatttgtt tttttaacac attgacgctt
     2521 tgatttgcta tttctgaaag atattgattc gtttcttcac ctaatgtctt attcaacctt
     2581 gaaccataaa tcaaacagga aattgtaatt actaagatac ttatgacagc aattatggac
     2641 aaaccatacc tattattttt tgcatcactt aacattatgc ttcctgcctt tctcgaatct
     2701 atgtatgtat aatcattatc ttatgacaat tattgtaaac attttataat aatacttatt
     2761 tttccatatt tactgtaatt atacaaaatt ataaacttta ttacaacaat aaattaatta
     2821 atatagatat tagggtttat attattgcat tatctatttt acaatcattt acatataaaa
     2881 aaatgtaata tcaaagtgaa acttgatatt acatttttgt tcttgtattt tgtttaattt
     2941 ttatgctaca ttttacttaa acactcattt gcaacttctt tatcatcaaa atgatgctta
     3001 actttaccta taatttgata agtttcatgt cccttacctg ttataattac aacatcatcc
     3061 ttttttgcca tacttatagc ttctgctata gcttgttctc tatcaacaac aacatggtaa
     3121 ttttcttttt tcttgtcaat tccttcaagt atatctttta ttattgcctc tggttcttca
     3181 gttcttggat tatcagaagt tataatacat aaatcagaat atttttgtcc aattgctccc
     3241 atcaaaggtc tcttttcagt atctctatct ccaccacatc caaacacaga aattattttt
     3301 ccttctgcaa attgttgagt tgtctttagt acattttcaa gtgcatcagg agtatgagca
     3361 tagtctacaa ttacacttat acctttatca tttggaactg tttcaaacct tcctgatact
     3421 ccattagaaa gtctaagacc ttctttatat attggtttag gtatgccaag tacatagcaa
     3481 gcagctataa ctgctaaagt attatatact gtaaacattc ctggaactgg tataaatatt
     3541 acttcttcat atgaaggtgt tattaatcta taagatactc catccgaatc tgattttata
     3601 tctctagcca taaaatcagc cttagtatca acaccataag tgtaacaagg tacatttata
     3661 ccttttatat tttcatatat cttttttcca ccttcatcat ctatgtttat tatatttgcc
     3721 atagtagtct taaaaaataa tttttctttt gcttttctat aatcctctaa atctttatga
     3781 aaatctaaat ggtctggtgt taaatttgta aatatgccta gtttaaagtc tgtttcatca
     3841 actctattta atgccaatga atgtgatgaa acttccatcg cacagtagtc acatccatta
     3901 tccaacatat tattaaaatg atattgtaaa tctatacttt ctggagttgt tgaattggaa
     3961 acaatgtctt tttctccgtc aaaaattttt atagttccta taagtccaac tttattctta
     4021 tttaaagtta atatctcatt tagtatagta gttatactag tttttccatt agtacctgta
     4081 actcctataa cgttaaattt ctgagatgga tgattataaa aattatcagc tacttttgcc
     4141 atatcttttc ttgtatcttt tacctttata aatgtacatc cttttatttc tacatcttct
     4201 tcaacaagaa atgcacttgc tcccttttct atggcatctt ttatatactt atgaccatct
     4261 gacacaaatc cttttataca tataaataat gtaccttttg taacctttct tgaattatat
     4321 tgtacattat taatatctat atttaattca cccttgacat tgattatatc caacccctgt
     4381 ataatatcat ttaatttcat aaatgctttc ctccctttaa actctttctg ctaaaattac
     4441 actacatata atattatatt aagaattttt tactatgtga atggttaaaa atgcttgaaa
     4501 tattgcaaaa aatgctctat aattttctat aatataactg aggaaaggtc ttctataaaa
     4561 actatggata ataaaatcgg gtacttaaac gataacttta aaatatttaa tattagagat
     4621 aaaaaagata tacaatttga ataccataat catgatttta ataagataat tatatttata
     4681 aatggaaatg taacttattt tgttgatggt attccctata aactaaagcc ttgggatata
     4741 ctctttatca gcaataatga aatccataaa ccagttatag attcaaatgt cttttatgag
     4801 agaattgcaa tatggattag cccagaattt atgaaaaaaa atagtgatgc tactagtgac
     4861 ttagaaaaat gttttaaaat agctacggag aataaatgca atagattgcg tttaaatata
     4921 catgatattg aatctattaa gagttttatt gagcaaataa atatttccga atccaatact
     4981 gaatttggaa atgaaatatt gagaaatgca ttatttatac aacttatgat attcttaaac
     5041 agaattttcc taaaaaatga aaatatggaa tcaacagaag acgtttttta tgatgaaact
     5101 attaagaaag tattaaacta tataaattca aatatagatg ataagctttc aatagatagc
     5161 atatcgtctg aattttttat aagtaagtat tatcttatga gaaaatttaa atctcatact
     5221 ggaacgtcta tacacaatta tatagttcaa aaaagattaa ttttagcaaa gtcactaata
     5281 accaatggtt accttatgag tgaagtttgt aataaatgtg gattcaacga ttattctagt
     5341 tttgtaaggg catttaaaaa aaactacggt gtatctccta aaaattatat aaacaataaa
     5401 aatcctttag atattactct tttagatgaa tagataattt atgtaaattt tattttaata
     5461 aaaacttgaa gtattagtgt atttatctta ctaatacctc aagtttttta atgttttaaa
     5521 atatatcaaa atataatgat atatatttct attacttaaa atatatatta aataaattgc
     5581 atgttaactg aacctattta tatatttatt aaatctcaca taacttttag ttatactatc
     5641 atatagctat cctatttttt caatatcata tcttaatata cttactctgt tctcctcttt
     5701 tgctgtagtt attccttttt taatgcttaa tttctgattt tgttctaaat tcgtaaatac
     5761 tacaggactt attgttgata gacaattttt ttcaatatat tctaagtcca ttttaactaa
     5821 tttttctcct gcttttactt catctcctac attcacaaat acttcaaatc catttccttc
     5881 taaatttaca gtttctatcc ctaaatgtat aagtatttct actccacttt ttttggattt
     5941 tattattatt gcatgcttag ttggaaatat aactccaata gttccatcaa ctggtgaata
     6001 aatcaatcca tcactagatt taattgcaaa tccatccccc atcagtttac ttgaaaatac
     6061 agaatctggc acttcttcta tatctaatat ctctccattt gttaatgaat caatcttttc
     6121 tactaaagca ttttccatat tggcaacttc catatttttt ttcactttat ttttatcaac
     6181 atttttagta acagtacttt caacctttgt ttttccctct attatatctt ttatatcaga
     6241 ctttattgaa tccgattttg gtccaaatat agcttgaaca ttgttgccaa taaccataac
     6301 acctgctgct ccaagtaatt tcaatttttc tttatctact ttatttatat catttacaac
     6361 caatctaagt cttgttatac acgcatctag gctctttaag ttgtctttat ttcctaaagc
     6421 aattaaaatc tctgatgcta attcattctt agacatttca atgacttcag cgttagtttc
     6481 tgactcatct tctcttcctg gtgtctttaa attaaatttt cttatcataa atctaaatac
     6541 actatagtat aatactgcaa aaccaatacc taccaagata gccaagtacc atggagttct
     6601 attaggaaga acaccaaata ataagaaatc tattaatcct cctgaaaatg tgaatccaat
     6661 tttaactcct aataattgca taattgcgta tgataaacct gaaaataaac aatgtattgc
     6721 aaataatact ggtgcaacaa ataaaaacgc aaactctatt ggctcggtta ttcctgttaa
     6781 aaaagaggtt aatgctgctg atgccattat acctgctaca aattttttct tttctggctt
     6841 tgcttcatgc accatagcaa gagctgctgc tggaagacca aacatcataa atggaaatgc
     6901 acctgtcata aaagtacctg ctgtgaaatt tactccatct ttaagttgag caaagaatat
     6961 cttttggtca cccattacaa gctcaccagc tttatttaca tattctccaa attgatacca
     7021 aaatggatta tggaaaacat gatgcaaacc aaatggtatt aatagtcttt ccaaagaacc
     7081 ttccataaat gctgctagtg tttgatttgc ttctataaca ttttttgaga aactaagtaa
     7141 tccctcttgt attggaggcc aaataaaaga caatataatt cctattgcta tagcaactaa
     7201 tgaagtaacg attggtacaa atcttttacc tgcaaaaaaa cctagatact gaggtaattc
     7261 aattttataa aactttttat aaagataagc agccactatc cctataataa taccaccaaa
     7321 tacccctgtt tgaattgttg gtattccaac aatacttgca tacatagaat tgtctaccat
     7381 atcttctgta acacccgcca taacacccat agatgtattg agaattaaaa aacctactat
     7441 ggctgccaat gctgcaacac cttctccttc agttaaacca acagctaccc caactgcaaa
     7501 tataagtggt aagttattaa atataatttg acctgaactt tccattatac ttattataag
     7561 ttgaaatgta ggattcgcca aagctggaaa atgagacaac aatgcgtctc ctgctaaagc
     7621 attactcacg ccaagtaata tacctgctgc aggaagtaat gcaactggta gcattagaga
     7681 ttttcctact ttttgtaaaa caccaaatac ttttttcatt tacttttctc tccttattat
     7741 tttatatttt tttaaataaa aaagact
//
LOCUS       DAELSY010000168         7651 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.168, whole genome shotgun sequence.
ACCESSION   DAELSY010000168 DAELSY010000000
VERSION     DAELSY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7651
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.168"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(320..883)
                     /locus_tag="KPG09_003303"
     CDS             complement(320..883)
                     /locus_tag="KPG09_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967867.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(1042..1755)
                     /locus_tag="KPG09_003304"
     CDS             complement(1042..1755)
                     /locus_tag="KPG09_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBG0967868.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            2047..3711
                     /locus_tag="KPG09_003305"
     CDS             2047..3711
                     /locus_tag="KPG09_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBG0967869.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDSKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(3818..4717)
                     /locus_tag="KPG09_003306"
     CDS             complement(3818..4717)
                     /locus_tag="KPG09_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBG0967870.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(4744..5382)
                     /locus_tag="KPG09_003307"
     CDS             complement(4744..5382)
                     /locus_tag="KPG09_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG0967871.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KVGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(5719..6717)
                     /locus_tag="KPG09_003308"
     CDS             complement(5719..6717)
                     /locus_tag="KPG09_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HBG0967872.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(6815..7390)
                     /locus_tag="KPG09_003309"
     CDS             complement(6815..7390)
                     /locus_tag="KPG09_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967873.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
BASE COUNT         2615 a         1189 c          826 g         3021 t
ORIGIN      
        1 attatatatt aagatatctt tatatattta tagttctgct tattcaacta tatttaacat
       61 tatacgtgta tgtataaata ctttttatac actgtttgtg ctatccatcc cgcacctaaa
      121 ggagtgggat ttttgcacta ttctgtatta tatatatcca aaaatattat aaaaatacaa
      181 atattagcag aaatttaaaa actgaagaaa gtattctaac taagatttac aaattcttaa
      241 tcctatcgaa gttaaactac tatcttcagc ctaattatct agcaatattt ttttatatga
      301 aaaactctaa atcaagtttt taaatgtttg cgatttttga tacataagaa tgttgtctag
      361 aattatattc tgataataaa ccttcatact ctaaggttaa gccagataaa ttataaaaat
      421 catttggcgt tacttcaaat gcttgtttat ttaaaatgtc tatctcactt ttttctaaca
      481 tgtcagcaac aaagtttgaa catacatatt tatattcaga ttgtctctga atattcatta
      541 gtaaatatat taatgccatt gtatcgtaat agtaatcttc tctataatca ctaattaatt
      601 ttatattttt atatagattt tcatattgta aattatctac ttctaacgaa tataccctac
      661 acacagtgtt ttttcttata gcatacaatc cttgatttat attttcttca acaaatcctc
      721 caataaatgg attccttgga tttagccttc caaaagaata cattggtttt agattttcat
      781 ttaatgatat tgacacatgt gtatacagct ttttacttat attccttatt aagtaagata
      841 atatagttcc tgtatatgtt gttacaatat agactttatt catcatttcc ccccaatcta
      901 gtaactaaat gatattatac acttacaatt taatatcata agatattcta acataataaa
      961 tgtggtttta caaattttga ttttaaggta ctttaatatt atataatata ataaaaatat
     1021 aaatataagt tttctatatt tttaaccaac agatgttttc ttcaaattaa tcagatgatt
     1081 tagtatatct tgaacctttc cagagtcctt taaatatcct aatgccccat ttatttgtga
     1141 ttttaaatct atttctactt ccatatccaa atcaacattt ccttttttta tagatatatt
     1201 tttaagtgtt attgggtaaa gataactttt ttccaaagta atcttatttc cattaacctc
     1261 aaatggaact tttttattgt agttacttaa tattttttct agcattttat cacttagttt
     1321 aaattttcca attttcacat tctcaagact tacaactaag tttccatcct ttaaagtagg
     1381 tctcaattca agttcatatt gactatttac caaaccaaat accttatatg gtcctgctac
     1441 ctttatttta ccatttttca tttcaacaaa attattttca agttcattaa ttccatgctt
     1501 ctgcatcaat gtatatatta aatttttaaa ctcttcctct gaaacagata tttttccaac
     1561 cattctaagt ggatttttta ctacctccat agaatttata tatgagcctt ttgataaata
     1621 ttcttcatct atgattttat tattttgtga actaacatca tatctttctt ttggtgtcaa
     1681 gatatataca acaattcctg caagtactaa aacaataaca attaaactta acaaaatctt
     1741 tgcaaccttt cccataatcg tctcctttac aaatctgtca tcagatttaa aataaattta
     1801 gataactaac ttcacataat attcttaaca atatacatac tataatacag tcttttaaaa
     1861 attaattcaa ccaatctaat gttttgtaaa taaactgtaa aaacatgttt ttagtataaa
     1921 agttaatatt ttgcataata ctcttaatta cacctttatt tttttctaat cttggattaa
     1981 aattaatatt atgcaataca gtgtagtgtt ctgtattata atcaaattaa ataggaggta
     2041 tagaatatgt caaatgaaac gaactcatct aactttataa agaatattat tataaacgac
     2101 ttagaaacag gaaaacatga cagtatcata actcgttttc cacctgagcc aaacggatat
     2161 ttacatattg gtcatgcaaa aagtatatgt cttaattttg gattagctaa ggagtttaat
     2221 ggaaaagcta atttaagatt tgatgataca aacccattaa aagaggatgt tgaatatgta
     2281 gagtccataa aagaagatgt aaagtggtta ggatttgatt ggaacgagtt aaattttgca
     2341 tctaactact ttgatgaaat gtataagaga gctttaatac taataaaaaa gggtaaagct
     2401 tatgtatgtg atttgacaca agaggaaatg agagaatatc gtggtactct aactgaacca
     2461 ggaaaagaaa gtcctcatag aaacagaact attgaagaaa accttgattt atttgaaaga
     2521 atgaaaaatg gtgaatttaa agatggtgaa aaaacattaa gagccaaaat agatatgtct
     2581 tctccaaata taaaccttag agacccaatt atatatagaa tatctcattc aacacaccat
     2641 aatacaggag ataaatggtg catatatcct atgtatgctt ttgctcatcc aattgaagat
     2701 gcaattgaag gaataactca ctctatatgt actttagagt ttgaagacca aagacctctt
     2761 tatgattggt ttgttaaaga atgtgagatg gaaaatatac ctagacaaat agaatttgct
     2821 agacttaata taaataatac tgtcatgagt aaaaggaaat taaaacaact tgtagatgag
     2881 ggtattgttg atggttggga tgaccctcgt gttcctacaa tatccggaat cagacgtaag
     2941 ggatatactg cagaagcatt gcgcaatttc tgtagtgaaa taggggtatc aaaagttaac
     3001 tctacggtag atagtcaaat gcttgattat ttcctaagag aaaacttaca accaaaggct
     3061 cctcttacta tgggagtttt aagaccttta aaacttatta taactaatta cccagaagat
     3121 aagattgaga tgcttgaaat agagaataat gctaaagatg aatctcaagg aaagagatta
     3181 gttcctttct ctagagaatt gtatatagaa caagatgatt ttatggaaga acctgttaag
     3241 aaatatttta gatttttccc aggaaatgaa gtccgtttaa aaggtgctta ttttgtaaaa
     3301 tgtactgatg taattaagga tgaaaatgga aatgtagttg aaatacatgg tacttatgac
     3361 cctgaaacta agagtggctc tggctttaca ggacgtaagg taaaatctac aatacattgg
     3421 gttgatgcta aatcagctat accatgtgaa tttagattat ttgaaccatt aattcttgat
     3481 gatatacctg aaaatgaagg taaacatttc ttagaacaaa taaatcctaa ttcattggaa
     3541 atattacaag gttttgttga acctacacag ataaaagatt ctaaaccttt tgataaattc
     3601 caatttgtta gaaatggatt ctttagtata gataataaat atacaactga cgaaaaattt
     3661 gtatttaata gaatagttcc tctaaaaagt tcttttaaac caggtaaata atcttacaat
     3721 caaaaaatga agacttaaaa tataaattac ttctactata aataaactta gttagaagta
     3781 attttttatt tgagtcttca tcatttactt taaatgatta acttttttca ctacagattc
     3841 ccataacttc tattccatct tttatgcatt ctatgttaag aggaaaatct ggtccttttt
     3901 ctccgtcaat atctgttata agtgaatctt cacattctat atgcaccttt ttagttttaa
     3961 agtatagtat ttcatttcca ttatattgat ctaaataatc acctctaagc acacttatca
     4021 aaactggtat tgattttggt attggcattc ctttaaaaat aattacatcc aaatatccat
     4081 catctatttc agccttatat gctagattta tatttcctgc tgttttgcca ttaaaaatca
     4141 acataagata catatctcca tcataataca tttcctcaga agatactttt atcttaaatt
     4201 tcctcatata caatgcttct tctattccct ttatataata agaaattctt ccaaatgaat
     4261 tttttaagtc tgtgtttatc ctttgtgata catctgtaaa cataccagca cttgcaacat
     4321 ttataaagta cttattattg actttcccta tgtctatttt ctttggtttt gaatttatga
     4381 tttcatttat agcttctttt atatcaaatg aaatttgaag agcatttgaa aaatcatttg
     4441 cagttcctgt aggcaaaatt ccaattggaa tttttatgtc taattctttc attgcattta
     4501 ctactaaatc tacagtacca tctccaccag ctatcaatat gtgttcataa gaactatcaa
     4561 cttccttaaa tgcattcttt acaggttctt taatagttaa tctatagggt atcaataaat
     4621 aatttttttc ttgatataat tctatgatag tgtctaaatt tgataatatc tttttttcac
     4681 ctgaattagg attgtatata agttttactt ttttcattat ttctccttac tcattttatt
     4741 cttttagtat ccaaaatctt catttaaaaa aatcacatgt attctttctg gtgttaattg
     4801 tctttttata agtgtataac tacagcatat tcttcctgga cttgaacagt ctatacatat
     4861 accagtagtc ttacatccag tatttgtact taatctttta caattggctg gagcagctat
     4921 tgtttttgtt ctttttatag cttcatctac atctttaact attttattta ctcctgctat
     4981 aactattact ttatcaggtc catatatcat agcagcaact ctattcccat tcccatctaa
     5041 attgtaaagt tctccatcct ctgttatcgc attagtactt gtaaaataag catctgcaga
     5101 aaatgacttt ctatatattt ctttcatttc ttctggtttg atatcttttg catatctatc
     5161 aagaaaatta tatttttctc ttataaaatc aatcactcct gtttcaaata aggtcataga
     5221 acctccacat cctactagag aacctacttt aactatttct tcaatttttt taagaagctc
     5281 ttctttgtta cttactaaat accctttcat attattgttt tccagagatt ttattgtttt
     5341 ttctattctt ttttcattaa gccaatttaa attttgatcc attttaactt cccccttatt
     5401 tttaatatgt acttaattat ttataaactt tattgtcttt ataagtttat tttagtacat
     5461 ctaaaaaaaa atacaatata aaacttaaat agttttatat aattttttcc aaaataactt
     5521 actcaatcaa aaaaggaaag tagatgtact attctacttt ccctatattg tatgtagtat
     5581 ttattgccta aaatctatat attaattata tgtttcatat tatataatat cacagttaat
     5641 taaaatgtaa tactatcttc tttttaaaca tataaattgt attattaaat ttaatatatc
     5701 caacttcaat aaatcttatt aatttgatga agaagatgct atagctgcac ttgttgctgc
     5761 cattacacat gcaatttgaa tagcaacaac aatatttaaa aatatattat tagttataga
     5821 gcctatatta ttctcatttc gtatcaaatc tgcataaatt gaagctacaa tagcagaact
     5881 aatcattact ctattacttt ttcctaaaga ccaatttcca aagccttttt cttctttaag
     5941 tttatttgaa acatttttta tctcttctat tatttcatct tctctataat ctaataatga
     6001 aattgctcct attattggat atccataata attcatttta caatcatttt ctttaaatgc
     6061 ttttttaagt tcaatagtct ttttacattt aagcatagtt ctatcatcac taaataaaag
     6121 aatttgggat aatgcttgta aatcatttcc tttataaaaa ccattttcat ttagaaattc
     6181 ataacatttt tctatgtatt ctaaatcttc atctaaattt tttgaatgaa ttgcaataag
     6241 tgctatatta caatagtcat cactagaagt tagaaatgga tgttttttcc tcatgtaatc
     6301 atatattatt ttcatttttg atatacatgt aagataatcc attttctctc tattttcata
     6361 aactattgat gctgtaagtg gtaaatatgt atcgccccaa aacttttcac ttttaagttc
     6421 tttgtatata tctaaaatca tcttaaaact actttctgga tttgattcaa acgaaagtag
     6481 tgttgccata taaaacatat tatatcctct aaaattagag aataatccag tatttttttt
     6541 aataagctct attcgttctt ccactaatct aggacttatt ctcttgttct taagagtaaa
     6601 tgcaagtgca caactatgtt gtatcaatcc catttgccaa gaacctttaa cttcatttaa
     6661 tgctttgtaa ttttctatta ataagtttac ttttccattt ataaacgatt gttccatatt
     6721 gccctcctag taatatatct cattgtacta atagttttat atttattata tatatattaa
     6781 atactacaat caatacatat tacttcaaag aacatcattc ttcaacaata atgtagcctt
     6841 ttttgtaatg tcttcccata ttattgactc tatcatagta ctctgattca ctttcattac
     6901 tccatttggg cacaaataca tctattgcat tagaattact aaaatactta ctagaccccc
     6961 tatccataac agtttttaat tttccatcta caataatctt agttccaaaa ctcaagtgat
     7021 tgtttgctac aaaaccatct tctaaatcat ctcctgtagc agttatcgct ccatatccat
     7081 tttcttgatt aagacttgtg taataactaa cttcaaaagt tataggtact aacttcttta
     7141 ttttaatacc taactgattt tgtagtttct ccttttgtac tttcaactta tactcatcac
     7201 tatttttata atcttccata ccttgattat acttggaatt aaagctttta cctgacttta
     7261 ctatagtatc tatatttact atagattctt tacaatgatc tgcatctatt tttgtttctc
     7321 catttaccaa taatggagtt aaacatacac tacatacaac acatgtaatc aatgcttctt
     7381 taagcttcat aaattccccc cttattattt tgatatatta gcatatttaa tattatatgt
     7441 ctatatttag atttaaatac cacacccatt tgctttaaga agttgtctta tatttaattc
     7501 accttaaaca cttataaatc ctctatctta gaatcatata gaataaagct tttatcgtta
     7561 aatatttttt attaatcttt taaaatctaa agctacttta tttaattttc tcttttttag
     7621 aatttaagaa ttaaaattct ataattttaa a
//
LOCUS       DAELSY010000169         7422 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.169, whole genome shotgun sequence.
ACCESSION   DAELSY010000169 DAELSY010000000
VERSION     DAELSY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7422
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.169"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            42..1019
                     /locus_tag="KPG09_003310"
     CDS             42..1019
                     /locus_tag="KPG09_003310"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBG0967874.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKTHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1012..1980
                     /locus_tag="KPG09_003311"
     CDS             1012..1980
                     /locus_tag="KPG09_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBG0967875.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2164..2754
                     /locus_tag="KPG09_003312"
     CDS             2164..2754
                     /locus_tag="KPG09_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0967876.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2773..3543
                     /locus_tag="KPG09_003313"
     CDS             2773..3543
                     /locus_tag="KPG09_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBG0967877.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3680..4648
                     /gene="dusB"
                     /locus_tag="KPG09_003314"
     CDS             3680..4648
                     /gene="dusB"
                     /locus_tag="KPG09_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBG0967878.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4757..5236
                     /gene="greA"
                     /locus_tag="KPG09_003315"
     CDS             4757..5236
                     /gene="greA"
                     /locus_tag="KPG09_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBG0967879.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5251..6780
                     /gene="lysS"
                     /locus_tag="KPG09_003316"
     CDS             5251..6780
                     /gene="lysS"
                     /locus_tag="KPG09_003316"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBG0967880.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7089..7283
                     /locus_tag="KPG09_003317"
     CDS             7089..7283
                     /locus_tag="KPG09_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967881.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NIWADRVRANSYSYENKIAK"
BASE COUNT         2957 a          753 c         1506 g         2206 t
ORIGIN      
        1 agaaaataag tcatgaaatt atgtcatatt agaggtgaaa tatgcgagat aaaataatag
       61 agattatatt aaataataaa tctgaattta tttcaggaga agaattatca aaacaattag
      121 gggtgtcacg tgcagctata tggaaacata tgaaagcctt aaaagaagag ggatataata
      181 tagaatctgt aaataaaaaa gggtatcgat tagcggaaaa tccaactgat ttattaagcc
      241 cacagaatat ttactataaa ttagatacag agtttattgg aaaaaatatt gaacatcttg
      301 acactataga ttcaacaaat gaacatgcta aaaggatagc tataaatagt gtagatggga
      361 ctgtgatttt aagtgaacat caagatatgg ggaaaggacg attgggaaga agatgggatt
      421 caaaaactca tgaaggaatc tggatgagta ttatattaaa accagatatg gaaccattca
      481 aagctccatt tataacttta atagcaggag caagtgtaac gaaagcttta aacaatctag
      541 gagcaaatac attaataaaa tggccaaatg atgttatttt aaatggaaaa aaaatatgtg
      601 gtatattaac agagttatct gctgaaattg aaagaacaaa ttatgttgtt cttggaattg
      661 gaataaatgt taaaataatg gattttgaac aagaaatagc tgacaaagcc acatcattgt
      721 ataaagaagg gtatatgtta tccagagtag atattgttag gaatgtattg actgaatttg
      781 aaaaattata tttagattat actgtaaata attccaaaga aaaaacctta aaaatatgta
      841 gagattattc tgcaattata gataaaaata tatatgtatt aaaaggagat aatagagaac
      901 tagtgaaatg tttagacata aatgaagagg gaaatttatt agttcaaaga agtgatggaa
      961 gtataaaaga aattatttca ggtgaagttt ctataagagg agaaaaagga tatgtataat
     1021 aatattttgg atatagaagg cttaaaggtt gggcaggttg aggatagaaa tggtcttact
     1081 gggtgcacag tagtaatatg tgaagaagga gctacttgcg gtgttgatgt aaggggttct
     1141 gctccaggga ctagagaaac cgatttatta gaccctgtaa atatggttca aaaagttcat
     1201 gcagtagttt tggctggagg ttctgcatat ggtcttgaat ccacttgtgg tgtaagtaga
     1261 tacttagaag aaaaaggaat tggctttgat gtaggtgtag gtaaagtgcc tatagttgta
     1321 ggagcagttt tatttgattt aggagtaggg agctttaaat gtagaccaga tttaaagatg
     1381 ggttatagag cttgtgaagt tgcaaatact gatattttaa atcaaggaaa ttatggagca
     1441 ggttgtggag ccactgttgg aaaaataaga ggtcaggaat atgctatgaa aggcggaata
     1501 ggtagctatt ctataaaatt agataatggt ctaattgtat ctgcattaat tgctgtaaat
     1561 gctcttggag atgtttatga aaatggtaag gttatagctg gtgctttaaa tgataaaaag
     1621 acagaaacaa ttgatacata taaattaatg aaacaaggtg aaagtaaagg tggatttagt
     1681 gtagataata ctacaattgg gataattgca acaaatgcta agttaaccaa agctggatgt
     1741 aaaaagatat ctcaaatggg acatgatgga tatgctaagt ctatttttcc tatccataca
     1801 cctcatgatg gagatacaat attcacaata tcaacaggag aaatagaagc agatataaca
     1861 ttagtaggtt cattggctgt agaggttgtg gaaaaaagta taataaatgc agtaaaaaaa
     1921 gcggatagtg tagaaaatat aatggcatat aaagatatag taaaaaaacg agaaatatga
     1981 tgaaattagc atatttttct tgtcaaattg ttgacaatgg caactcatct tgataaaatt
     2041 attcttgtta gagcgggcat cctacaatgg tagcttgact catagttata agaaaaaaac
     2101 gaaaaaaatt aaaaaattaa gtagtccggc atcttaatga gctggaacgg aggtgtattt
     2161 tttatgatga acacaagtgc atcagtaaga aaagtaaatg taagaaagat gactataata
     2221 ggagtactat cagctatttc tattatgcta tctatgactc cattgggatt tattccagtt
     2281 ggacctacaa aagcaactat aatgcacata ccagttatta ttggagctat tatggaagga
     2341 ccaatagttg gggcagcaat aggatttata tttgggatat caagtctctt aaatgctata
     2401 acaaacccaa cagtaacatc ttttgttttt ataaatccat tagtttcaat attaccaaga
     2461 gttatgatag gtgtattagc ttattatgta tatcagttaa taattaaggc tacaaataag
     2521 gtatacatat caggtttaat tacaggggca ataggttctc tgttaaatac agctggagtt
     2581 ttaggaatga tatatgtctt atatgctgac aagtatcttc atgcaatggg taaaagtggt
     2641 tcagcaggaa aagtaataat ggctcttgcc gcagcaaatg gggtgccaga agcaatagta
     2701 ggggctttag tagtagctgc tgttgccact gtacttaaga aaagcaaaaa atagaaagaa
     2761 ggttaatgac atatgcttct agtatttgat gttggaaata ctaatatggt tttaggtata
     2821 tataaaggtg acaaattagt taattactgg agaattaaaa cagataggga aaaaacgtct
     2881 gatgaatatg gaatcctgat aagtaaccta tttgattatg ataatgtgaa tataagtgat
     2941 attgatgatg ttataatatc atctgtagtt ccaaatgtca tgcattctct tgaaaacttt
     3001 tgtataaagt actgtaaaaa acagccatta atagtaggtc caggcataaa aacaggtcta
     3061 aatataaaat atgataatcc aaaacaagtt ggggcagata gaatagttaa tgctgtagca
     3121 gggatagaaa agtatggagc accaagtata cttgttgatt ttggaacagc aactacattt
     3181 tgtgctatct ctgaaaaagg tgaatatttg ggtggaacaa tagcaccagg aataaaaata
     3241 tctagtgagg cgttatttca aagtgcgtct aaattaccta gagtagaatt agctaagcca
     3301 ggtatgacta tttgtaagag tactgtatca gccatgcaat ctggaataat ttatggatat
     3361 gttggtttag ttgacaaaat aataagtatg atgaagaaag aattgaattg tgatgatgtt
     3421 aaggttatag ctacaggtgg attagctaaa ctgattgctt cagagacgaa aagtatagat
     3481 tatgtagatg gttttttaac actagaagga ttgagaataa tatatgaaaa aaaccaagaa
     3541 taattaatat acagggaatt gggttatata tccaattccc tttttatgat agatttctag
     3601 ctatatgtgg tatattataa tgaggaaata agaatttaaa gttaaatcac aaaataaagg
     3661 caaaaatagg agaaaaacaa tgaaaatagg aaatttagaa ttgaaaaata aagtttttct
     3721 atcacctatg gcaggtgtaa cagaccttcc atttagatta atttgtaaag aacagggatg
     3781 tgggctttta tatacagaaa tgataaatgg taaggcactt tgctatgatg atgaaaatac
     3841 taaaaaaatg cttaagatag aagaggaaga acatcctgta gcagttcaga tattcggttc
     3901 agaaccagag tttatgggaa gagcagcaga gataatgaat gattattcaa atgagattct
     3961 cgatataaat atggggtgtc cagctccaaa agtagttaaa aatggagatg gttcagcact
     4021 tatgaaaaat cctaagcttg cagaagaggt attgagggca gtagtgaaga actcaaaaaa
     4081 gccagtgact ttaaagattc gaaaaggctg ggatgataat agtgtaaatg ctgttgaaat
     4141 agcaaagata gcagaggatt gtggaataag cgctttagca atacatggaa gaactagaga
     4201 acagttttat acaggcaaag cagactggga tataattgca gagattaaga aaaatttgag
     4261 tatacctgtt ataggaaatg gtgatgtatt tacaatagaa gattcaataa atatgttaga
     4321 caaaactggt tgtgatgcta taatgatagg taggggtgct caaggtaacc cttggatatt
     4381 taaaagaata aaccattata tgaatacagg cgagattttg ccagagccaa ctcttaacga
     4441 aaaaataagt actgccatta agcatttaaa acttgcagta gaagaacatg gagagtatgt
     4501 agctgtaagg gaaatgagaa aacacatcgc ttggtattta aaaggactta gaaactctgc
     4561 aaaattaagg gatgaaatta acaaaataga agactatcaa gaagttgtgt cgaaattgga
     4621 gtactatatg gaagactcct tgacataaaa ataatagtga cttataatat agcgcataaa
     4681 ttaaaaaaag attataaaat ttttaaaata taattaggaa aattcctaag aaatttgtga
     4741 aggagttgta tttataatgg aagaaaataa agagttttta ttgacacaag agggatatga
     4801 taaattagaa gaagaattag aaaacttaaa ggttgtaaag agaaaagaag ttgcagagag
     4861 aataaaagtt gctatatctt ttggtgattt atctgaaaat gctgagtatg atgaagctaa
     4921 aaaggaacaa gcacaagttg aagaaagaat tttaaaatta gaaaatatgg ttagaaaagc
     4981 tgttatcata gatgaaagca aaatagacct taatgttgtt acaataggat caatagttaa
     5041 agttaaggat ttagaatttg atgaagaggt tgaatatact atagttggtt cgacagaagc
     5101 agacccatat gatggtaaaa tatcaaacga atctcctgta gggaaagcac ttttaggtag
     5161 agctgcgaaa gaagttgtgg aagtgcaagt tcctgatgga gttgctaagt ttgaaatatt
     5221 agaaataaga agataatttg gaggaaaaaa atgaaaaata atcaacaaag caatgaagaa
     5281 gcgcaaattc aagaagacct tagtgaagta cttcaagtaa gaagagataa actaaaaaaa
     5341 ttacaagagt caggtagaga tccttttaaa gaaagtagat atgatagaac tcattattct
     5401 atggatataa aagataactt tgattctctt gaaggtaaaa ctacaaaaat agcaggacgt
     5461 ataatgagca agagaataca agggaaagct ggctttatag atattcaaga ccaagaggga
     5521 agaatccaat cttatgtaag actagatgct ataggagagg aagaatatag tgtattttca
     5581 acatatgata ttggagatat agttggaatt gaaggagaaa tctttaaaac taaaaaaggt
     5641 gaaatatctg taaaggctaa atctgtagta ttactatgta aatctttaca agttttacca
     5701 gaaaaatatc atggattaaa agaccaagaa ctaagatata gacaaagata tgttgactta
     5761 atagtaaatc cagaagtaaa aaatgctttt ttaattagaa caaaagcatt aaaagcatta
     5821 agagcatact tagatgatag aggattttta gaagttgaga ctccaatatt aaatactata
     5881 gcaggtgggg caaatgctag accatttata actaatcata atactttaca tataccaatg
     5941 tatttaagaa tagcaaatga attgtattta aaaagactta ttgttggtgg atttgataaa
     6001 gtttatgaaa tgggaagaat gtttagaaat gaaggtatgg atttaaaaca taatcctgaa
     6061 tatactgcaa tagaattata tcaagcatat gcagactata ctgatatgat ggaaataaca
     6121 gaaaatgtta ttgcacatat ggctgaagtt gctacaggaa gtatgatagt taactatcaa
     6181 ggaacagaaa taaactttac accaccttgg aaaagaatga gtatggaaga ttgtgttaaa
     6241 gaatattctg gtgtagattt ttctactata aatacagatg aagaggcact agaagttgct
     6301 agagaaaaag gtatagagat aaaacctgga atgagaagag gagaagtaat aaatgctttc
     6361 tttgaagaat ttggagaaga taaattaatc caaccaacat ttataactca tcatccagtt
     6421 gaagtatctc cattatctaa gagaaatgtt gaagacccaa gaagaactga tagatttgag
     6481 gcatttgcta ataagtggga gttagctaat gcattttctg aacttaatga ccctattgac
     6541 caaaaaggaa gatttataga ccaattgaga aaaagagaat taggtgatga tgaagctttt
     6601 gaaatggatg aagatttctt aaaagcatta gaggtaggtc tacctcctac aggaggacta
     6661 ggaataggta tagatagagt aattatgtta ttaactaact ctccatctat aagagatgta
     6721 ttattattcc cgactatgaa acctattgat aacaatccaa ataaagaaga agaaaactaa
     6781 gaaatattag cattctacta agttttaaat aaagtttagc tattaaatta gcaataaata
     6841 gctaaatttt gtttaaaatg tcagtgtaga atgcttttta tatgcttcaa agattctttt
     6901 agatatttaa tttataaatt accaacaaac gttagaaatt gatattagtt tttgttgatt
     6961 gagactttat atttagaaaa tatgattgaa agtaatttat tatgaaaata tttagaataa
     7021 tttatgagaa ggtaaaaaga ataatttatg agaatactat atataaaaaa ttattggggg
     7081 aatgtctgat gtttcaagta attaattttt tatgtttatt ttgtgttgga tttttggttc
     7141 ttgatgttat gatatatttt tgtaaggatg caatggatat ttttgagggt acaactaatg
     7201 ttaataaagt taagagtata aatatttggg cagatagagt gagagctaat tcatattctt
     7261 atgaaaataa aattgcaaaa taggtaaaat tatatatgca tgtattgttt gtaatatcaa
     7321 gggttatatc tattagtttt aattatattt attaaaaaaa ccaaaagtta taaccttttt
     7381 taaaataaaa aacaaaaaaa gtattgacga aatattttta ag
//
LOCUS       DAELSY010000170         7411 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.170, whole genome shotgun sequence.
ACCESSION   DAELSY010000170 DAELSY010000000
VERSION     DAELSY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7411
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.170"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            177..1370
                     /gene="nifV"
                     /locus_tag="KPG09_003318"
     CDS             177..1370
                     /gene="nifV"
                     /locus_tag="KPG09_003318"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBG0967882.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVI
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1395..3320
                     /locus_tag="KPG09_003319"
     CDS             1395..3320
                     /locus_tag="KPG09_003319"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBG0967883.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSNVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3342..4337
                     /locus_tag="KPG09_003320"
     CDS             3342..4337
                     /locus_tag="KPG09_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBG0967884.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGAEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4664..5137
                     /locus_tag="KPG09_003321"
     CDS             4664..5137
                     /locus_tag="KPG09_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0967885.1"
                     /translation="MVGSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5171..5665
                     /locus_tag="KPG09_003322"
     CDS             5171..5665
                     /locus_tag="KPG09_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0967886.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5762..6283
                     /locus_tag="KPG09_003323"
     CDS             5762..6283
                     /locus_tag="KPG09_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0967887.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMYDDNIEADKYIREELKVPSKYSVECIISIGYSDEEKKAYND
                     SDLDYKKVHFNNF"
     gene            6472..6783
                     /locus_tag="KPG09_003324"
     CDS             6472..6783
                     /locus_tag="KPG09_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG0967888.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6811..>7411
                     /locus_tag="KPG09_003325"
     CDS             6811..>7411
                     /locus_tag="KPG09_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0967889.1"
                     /translation="MKYSYKKCIIDSILLMFIIQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFG"
BASE COUNT         2980 a          747 c         1447 g         2237 t
ORIGIN      
        1 gcaataaatg atacaaagta atataataga ttacaagata tgaaaagcta aaaaaataaa
       61 ttgcaaatga aattaaaatg aataaaaaat aaaaaaataa tcataagtta gaggtatcaa
      121 tgaaaaataa gacttaatgt ttttaaaatt aaaagtatac taaggggggc attaatatgt
      181 gcgttatatc aaaagataga gcaaaggaaa taaaaatagt agatactact ttaagagatg
      241 gagagcaaac agctggggtt gtatttgcaa atagagaaaa aataatgatt gctgaaatgt
      301 taagtgacct aggtgttgac caaattgaag ttggtatacc tactatgggt ggagatgaaa
      361 aaaatgtaat aaagcacata tgtagtagaa atttaaaatc agatataatg gcttggaata
      421 gagcagtaat aaaagatgtt gaagaatcta tttcttgtgg tgttgatgca gtagctatat
      481 caatatcagt atctgacatt catatagaaa ataaattgag aacttctaga ggctgggttt
      541 tagaaaatat ggctaaaaca gttgaatttg caaagaaaaa tggattatat gtttcagtaa
      601 atggagaaga tgcttcaaga gcagatatag acttcttaac tgaatttata aatgttggaa
      661 aacaagcagg ggcagataga tttagatact gtgatacagt aggagttatg aatccttttt
      721 ctataaagaa tgcaatagaa actctttatg aaagaactaa ttttgatata gaaatgcata
      781 cacataatga ctttgggatg gcaactgcaa atgcacttgc aggaatagca gcaggagcaa
      841 attatgttgg tgttacagta aatggattag gtgaaagagc aggaaatgca gcattagaag
      901 aagttttaat ggcacttaag tgtgtttata aatgtgattt aaataatata gatactagaa
      961 aatttagagg aatatgtgag tatgtagcac aggcatcagg aagaatactt cctacatgga
     1021 aaccagtagt tggggataat atgtttatac atgaatctgg tatacatgca gatggagctt
     1081 taaaagaccc gcataactat gaaccatttg acccaagtga agtaaatctt gaaagaaaaa
     1141 tagtaatagg taaacattca ggaagagctg cagttataaa taagttgagt gaatatgaaa
     1201 tgtatatttc tccagaaaac gctaccaagc tattaaatgc tataagagct acatctataa
     1261 gattaaaaag aagtttaatg gataaagaaa ttcttcaatt atattgtgat atactagcac
     1321 atgagaaagg cacaacagaa gaagaagcag ttagaggttc atatatttaa gatataattg
     1381 tttaggagga aaatatggga gataatatag tttataaaat aattaaaaaa catatagtag
     1441 atggagaagc tgttgctgga agttcaattg gtataaaaat agaccaaact ttaacacaag
     1501 attctactgg aacaatgaca tatcttcaac tagaagctat gggaatagat aaagtaaaga
     1561 ctaaaagaag tgtggcattt gtagaccata atatgttaca acaaggattt gaaaatgcag
     1621 acgaccataa gtatatacaa acagtggcag ataaatatgg agtttatttc tctaagccag
     1681 gaaatggtat ttgtcatcaa gtatttttag aaagattttc aacaccagga gatacacttt
     1741 taggttctga tagccacaca cctactgctg gaggtgtggg catgatggct attggagctg
     1801 gtggattaga tgttgcttta gccatggcag gaggagcata ctatataaaa gcaccaaaag
     1861 tctgtaaggt gaatctagtt ggaaaattaa ataatatggt atcatcaaaa gatataatat
     1921 tagaagtgtt aagaaaacaa actgtaaaag gtggagtagg taaagtttat gaatatggtg
     1981 gagaaggtgt caaaagccta tctgttccac aaagagctac aataacaaat atgggggctg
     2041 aacttggagc aactacttca atcttcccaa gtgatgaaaa aacactagaa ttttttaaga
     2101 gtcaaggaag agaagacgca tggatagagt taaaaccaga tgcagatgca gtttatgatg
     2161 aagagattac tataaacttg gatgaattaa aaccattagc tgcaaaacct catagcccag
     2221 acaatgtaga tgaagttgaa aatataggca agattaaaat agaccaagtg gcaataggaa
     2281 gttgtacaaa ttcttcttat gaagacttaa tgaaagtagc acaaatcctg aaaggaaata
     2341 aagtacataa ggatgtgagt ttggtaatag caccaggttc aagacaagtt atggagatga
     2401 ttgctagaaa tggggctttg gcagatataa taagtgctgg agctagaata ctagaaaatt
     2461 cttgtggtcc atgtattggt atgggtcaat ctccaggtac tgactctgtg tctttaagaa
     2521 ctttcaatag aaatttctat ggtagaagtg ggactttgtc agcacaagta tatttagtaa
     2581 gtccagaagt ggcagcagta agtgcaatca agggagtgct tacagaccca agagaatttg
     2641 atattaaatt tacaaattta gatgtaaatg aatttttgat agatgatagc atgataatta
     2701 aacctgctga tgtaggaagt aatgtagaag tagttagagg acctaatata aaacctttcc
     2761 ctcttaatac agagctaagt cagtctatag gtggaaaagt tatattaaag acagaagata
     2821 atataactac tgaccatata atgccatcaa atgctaagtt acttcctttt agaagtaata
     2881 taccgtatct tgcaaactat tgtttcaata ctgtagatac agaatttcct cagagagcaa
     2941 aagacaataa tggtggattt atagttggtg gagataatta tggtcaaggt tcaagtagag
     3001 aacatgcagc tttagcacca ttatatctag gtgttaaagg ggtaatagtt aagagctttg
     3061 caagaatcca caaggctaac ttgataaata gtggtataat tccaatggag ttttgtgatg
     3121 agaaagatta tgaaaatata tctcttttag ataatctgga gataccaaat attttagaca
     3181 atctaggtag tggaatttta gaagttaaga atacaactaa aggaacttct tttaaggtca
     3241 aagtagaatt gtcagcaaaa gaagttgacg tattaaaagc tggtggaaaa ttaaactaca
     3301 ctaaaaatca agctaattaa tatgaaaaga gggaatattg aatgtataaa gtgacactta
     3361 taccaggaga tggaatagga ccagaagttg cgaaagctat gaaaaaggta gtagaagcta
     3421 ctggagcaga aatagaatgg gaagaagtta atgcaggaga ggcagttatt gaagagtatg
     3481 gtactccact tcctgaatat attatagata gtataaaaaa gaacaaaata gctataaaag
     3541 gcccaattac tactcctgta ggtaaaggat ttaggagtgt aaatgtaact ttaagacagg
     3601 cattagattt gtatgtcaat ttaagaccta ttaaaagttt taaggggata aaatcaagat
     3661 atgaagatgt tgatttagtg gttgtaagag aaaatacaga agatttatat gctggaatag
     3721 aacataaaat aggtgattat gcagctgaaa gtataaagat aataacaaga agtgcaagtg
     3781 aaagaatcgt tgattttgca tgtaactatg ttaaagataa taagagaaaa aaagttacag
     3841 ctatacataa ggcaaatata atgaaaatgt ctgatggatt gtttttagat gtgtttagag
     3901 aagttgcatc aaaacatggt gtagaatatg atgatttgat tgtagatgca gcagctatga
     3961 acttagtttt aaatccagaa aattatgatg taatggtaat gccaaatctt tatggagata
     4021 tactatcaga tttaggtgct gggcttgttg gaggtttagg tataattcca agcgcaaata
     4081 ttggtaaaga ttgtgcgata tttgaagctg ttcatggaag tgctccacaa atagcaggtc
     4141 aaaataaggc taatccaaca gcacttattc aatcatcagt aatgatgctt agatacttag
     4201 gtgaatatga aaatgcacaa aaaatagaaa cagctttaga aaaagtattt cttgaaggaa
     4261 gtaaattgac tgtagattta ggtggctcag cttctactac agagtttgcg gatgaggtgt
     4321 gtaagtatat tgtatagatt ttatttaggg aaaaagtgaa atatagattg aaggaaagag
     4381 gctttctcaa aatagagata agcctttttt tattggaatg ataaaatata tttgatttta
     4441 tacaaaagag agttcctcat aaacgaaaaa gttaattttg ggatatccat tttaatttta
     4501 aaattaattg attaatacgc aaagaaagat taaacaaatt tattattttt aattgaactt
     4561 tcaaccaaaa tatggtaaaa tttaaaaaag ttgaactttc aactaaaaat aaatatattg
     4621 attatgtgaa attctataaa acagaaacta tttgacttta gtcatggtag gttcagtgga
     4681 ggtgtgtttg attaagactt tagatagtaa tatattaaga gaagtaggta ctttatctag
     4741 agcagtaaat tcaataaatg atattaagta caaggaatta aagttacaaa aaggacaatt
     4801 tacatttttg acaagaatct gtgaaaatcc tggtataaat cttgtggaat tatcaaatat
     4861 gttaaaggta gacaaggcta caacaaccaa ggctatacaa aaactaataa aggcaggata
     4921 tgtagataaa aagcaggatg agtttgataa aagaggatat aatttaactc caactgataa
     4981 atctttggaa gtttatgagt tgataattga agaagagaat agaagtatag agatatgttt
     5041 tgataatttt acagatgaag aaaaacaagt ggtaactaag ttattagaaa aaatgagcaa
     5101 aaacgtagaa aatgaatggt ttaaagtaaa gagatagtaa ataaatacat taacttaagg
     5161 agatattttt atgattagaa aagcgaatat gaatgattta gaaagcatta tgaaaattat
     5221 aaaatctaca gtggaagaaa tgaagactta taataataca caatgggatg aaaactatcc
     5281 attagaaaaa gactttgttt cagatattaa gaaacaagat ttatatattt atgaagtgga
     5341 tggagaagta gcaggattta tatgtcttaa ttatgaagag ccagaagaat atttaggctt
     5401 aaattggtca tctaataaaa aagcaatggt aatacataga atggctgtaa atccaaactt
     5461 tagaaagaca ggaatagcat ctaagttagt tgattttgct gaaaaattag ctgtagaaaa
     5521 caatgtatca tacttaaaga gcgatacata ttcaataaac tcaaaaatga atttattact
     5581 tacaaagtgt ggttttataa aaatcggaga aatgagtttt ttaggaaagg agaaatcttt
     5641 ctactgttat gataaaatat tataaagagc atttataata caaggggtaa ataatagatt
     5701 ttcagcatta gtaagggcat aatataaccc agtatatgct attatgaaag ggatgattta
     5761 tatgattgga tttctaaaga aaagaagaag tattagaaaa tacaaggatg ttgaagtaga
     5821 aaaagaaaaa ttagataaaa tattaaaagc tgctctttta gctccatcat caaaggggct
     5881 tagaacatgg gaatttatag tggtagatga taaagaaaag ctaattaatt tatctcaatg
     5941 tagaactaag ggtggtggat ttttcttaaa aaatgcacct ttagctattg taattattgc
     6001 agataaagaa aaaaatgatg tttggataga ggatgcaagt atagctgctt catacataca
     6061 acttcaagct catgaactag gtttaggttc ttgttggata caagtaagaa atagaatgta
     6121 tgatgataat atagaagctg ataagtacat tagagaagaa ttaaaagtac ctagcaagta
     6181 cagtgtagaa tgtataattt ctataggata ttctgatgaa gaaaagaaag catacaatga
     6241 ctctgattta gactataaaa aggttcattt taacaacttt tagtatataa tatgttgtat
     6301 taagacatta tatttttttc tattgttaat atatacttaa aattgtaaaa atatatctga
     6361 attttactaa cttatctagg ggttatgtac aaggatttta aagataaata tagaaattaa
     6421 tgtttataat cgatacataa cttgagtaac aaaatttaag gaggttaaag tatgggtata
     6481 tttaaaacaa aaatggatga ggactggaaa gtaaactaca ttaaagagtt taatgaaatg
     6541 agagattctt atgagagtaa acttcaaaaa aaacagtttg aggtagatag tttgaaatca
     6601 gaattggata gactaagaag ctataaaaat agccttaagc caaaagaaaa acaaattact
     6661 gatgatgata taaataatat aaagagttta agaagagatg gcttaagcta taaagaaatt
     6721 tcaaatcaaa caagttggag caaagcaact gtaagtaggg tattaaatgg actttacgat
     6781 tagcatatac atggggtgat ttaaattggg atgaagtata gttacaaaaa atgcataata
     6841 gattctattt tattgatgtt tataattcaa atattaagga tgattttgaa ttatgtactt
     6901 ctaagtcaat ttgaatttac tcttgaaaat tttaatatta taaacttaat ttcttttaca
     6961 ttagttggtt tatcgttaat tttattttta aaagataatt ctttgtataa caaggttaga
     7021 aatagaaaaa tcacagaagc ttttgaagaa aataaaaaca atatacttat tgaaaagtgt
     7081 aagcttatac tttttgttgt ggttttatct ttagcaataa ttgtaactta ttgtactaag
     7141 ggatatgtac tttttaatgt aactatgatg actttgtctg tattgatagt tccaatattt
     7201 gaagagttat tttttagaga atatatatgg aactatctaa gtaattttat aaagtcaaag
     7261 agtaaaatta tctgtataac atctatacta tctggaattt ataatatagg atacatagat
     7321 gttataagaa attatgtaat attgtataac aattcaagct acacctttga agttattatt
     7381 tcaaaaatta tgataggtac tgtttttggt a
//
LOCUS       DAELSY010000171         7408 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.171, whole genome shotgun sequence.
ACCESSION   DAELSY010000171 DAELSY010000000
VERSION     DAELSY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.171"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(518..1330)
                     /locus_tag="KPG09_003326"
     CDS             complement(518..1330)
                     /locus_tag="KPG09_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0967890.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPVNYPNLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNDEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            complement(1700..2602)
                     /locus_tag="KPG09_003327"
     CDS             complement(1700..2602)
                     /locus_tag="KPG09_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0967891.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDGQISFFD"
     gene            complement(2652..3113)
                     /locus_tag="KPG09_003328"
     CDS             complement(2652..3113)
                     /locus_tag="KPG09_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBG0967892.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            complement(3165..3620)
                     /gene="def"
                     /locus_tag="KPG09_003329"
     CDS             complement(3165..3620)
                     /gene="def"
                     /locus_tag="KPG09_003329"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG0967893.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(3592..3855)
                     /locus_tag="KPG09_003330"
     CDS             complement(3592..3855)
                     /locus_tag="KPG09_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0967894.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(3952..4668)
                     /locus_tag="KPG09_003331"
     CDS             complement(3952..4668)
                     /locus_tag="KPG09_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0967895.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGTLAFIITMILLIFFSLTKISFLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYIYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            complement(4665..5342)
                     /locus_tag="KPG09_003332"
     CDS             complement(4665..5342)
                     /locus_tag="KPG09_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0967896.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILISIIIIIYFITYYFICSSWK
                     KVGGVKL"
     gene            complement(5339..6184)
                     /locus_tag="KPG09_003333"
     CDS             complement(5339..6184)
                     /locus_tag="KPG09_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967897.1"
                     /translation="MIKVDDLSFSYTDRNFLQNINFEVEKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIKKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            complement(6298..6888)
                     /locus_tag="KPG09_003334"
     CDS             complement(6298..6888)
                     /locus_tag="KPG09_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0967898.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRVVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            7177..>7408
                     /locus_tag="KPG09_003335"
     CDS             7177..>7408
                     /locus_tag="KPG09_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967899.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
BASE COUNT         2620 a         1158 c          686 g         2944 t
ORIGIN      
        1 aggttccaat attttaatta tgtcaactat atctcttata gttttaataa tctcatcaat
       61 tggtttatgt gcttgttgca ctttatcaat agtattaatt aaatccattt acacatctag
      121 cacctgtatg gcaatttagc attatcctta tcttatactt tgttgtaaat tcttgattac
      181 ataattctat tatctaactt atagctgtgc aatctacatt atctgtaaat atctttgctt
      241 tatatatttt ccctgaacct ctaacattaa tataacagcc tttttgctat acacttttaa
      301 ctatatactt aattttaata acattacttg tttataaaaa tatactatag ataaatttca
      361 cataaaaata ataattcaag tattgatttt tatataagaa aatttgtaat gtactaaaaa
      421 taacataaaa gtttccattt ttcaatatgt aagcttttct tatgttattt tattgttaaa
      481 ggtttcaagt ttttatttat tcattttaaa aaaattatta tttttgtaaa actaatccca
      541 tagtcatact atcgtcagaa tatgcttcac ccttaacatt actaaaaaac tcaacagact
      601 taaatccaat attttcaact tctaataaga aactttcttt tgaaaaatag tgattccaaa
      661 cataaaatac attttcatct ttttcggtta ttacaagagt ttgttctaat gtattataat
      721 cattgtattt acaattatct tgtagacaca tatatttttc attactccaa aatcctccat
      781 cttcatttat ttcccaatat ttaatttcct caaaattatt gtatttattt attgtaaata
      841 catctaatat taattttcct ccaggtttta aactatcata aattttttcc attaataacc
      901 ttctattttc agttgacaaa gctccataat cacaataaat taaagtagcc aaatcaaatt
      961 catcattata gttcatattt aaataacttt gaaacaagta atttatgttt aaattagtct
     1021 ccttatttct attttgtgca tagttaatag aacgttttga gaaatcaatt ccagttacct
     1081 tataaccttt ttgtgcaaat ttctctgcat ataaacctgg accacaacct aaatcaagta
     1141 aattaggata attaactgga tttgcaactg taactatcca atttacagaa tcctcgataa
     1201 agttaaaatt tctacttgct ccttcaaaat tagtgtctaa atgtgctttt aataattgtt
     1261 ttgaaatata ctcatcatcc caaaaattaa tttcactttg tttataaagc tctggtcttt
     1321 ctaaatacat acataatttg tttaacaaaa cataccactc cttaaaattt atttttaaaa
     1381 taaaaaaacc acagagttta ctctgtggtt taacttcaaa aagatgagtt taaataagag
     1441 taaaatagaa ccaaatattt ttaagcacaa caataaaact atagctttac tttgtttgct
     1501 tattaaattt gattttattc actcttttcc agcaatactt atatcaacga ttttatgata
     1561 tcaaatatca ccgaacaaca tcatcctttc atatttttat tttatgaatc aattatatat
     1621 ttaaatttta tatttgtcaa taaattttaa ttttaataaa ttttaatcat catagtaaaa
     1681 aaaaattttt tatataaaac taatcaaaaa aagatatctg tccatctcct gagtttttta
     1741 acttttcctt cattatttta gagtaactgc ttgatagaga atatttatat ctaagttcat
     1801 taaccatttt atacaattga tttttatact ccctattaag tgaacctgca ctatatagta
     1861 ttgataattt atcaaataat tcaggaaact cttttttaac aaagtcaaaa aaaacacctc
     1921 tagttatacc ccttaagtat aaagtcccag gtaaaacata atgtacatta cattcttttg
     1981 catgtctaaa tagcgaattt atattatcaa aatcatctgt aatatatggt ataattggca
     2041 taatatgtag accaactgat gcgttagttt ttctaaattc ttttaacatt ttaaatcgtc
     2101 tcattgaatc cacaccattt ggctcaataa gcctttgtgt atcttcattt actgtggtaa
     2161 ttgtagatgc tacattgata tatgtaatcc tagatagctt atcaattaaa tcgtaatctc
     2221 ttaaaattaa atctgacttt gtagaaataa tagctggtgt cttgtatttt atcagcaggt
     2281 ttaatatttc tggcataatt ttatagtcag cttctattgg ttgataacta tctgtaactc
     2341 ctcctatatt gattacttct ttcttccatt tactgctttt aagttgtctc tcaagttttt
     2401 ctactatatt tgtttttaca taaatctctt caaaataatt attggagtta atgtacttat
     2461 gagagtaaat agcataacaa tatttacatc catgctcaca tccacgataa atatttaaat
     2521 cccatttata tggcatactc ctttttaatt tattacaagc tatttcacaa tttattggaa
     2581 tatattttat tttattatac aaataatcac ctctttatat acaataatac tataatgtta
     2641 atctatagta attaaaacat gatatttcca gaccacaatt cataatcttt actatattta
     2701 caaagtaata tattccattc atttattggt attcctccac atgttgcttt ttcttctaaa
     2761 ttatataagc attgctttaa atacatttta ttatgccaat catcatataa attttctaaa
     2821 tttaaactat gttcattaga tatatcattt gcaaaatttc tatttttcca ttttaatata
     2881 cttgtatatt taagttcatt aggtttataa tttcttttta acatttcatc atgtacaatt
     2941 tttgtataaa atttaaattc atctatacta taattcaata ctttattcac taatagatgg
     3001 tttggagtac cttttttatc tatagaacct tttatagcaa gtagctctct ccattgactt
     3061 actaattgat ttttaggtaa tacatcaatt aaatccttat gccataatct cataatttaa
     3121 ctccttacta taaaatattt tataaattca ataaccaatc tatcttacaa ttttacatgt
     3181 tcaataattt tatctgtaaa aactatacca tttaaatggt ctatctcatg acaaagacat
     3241 tttgccataa aacctgaacc tttaatttct attttctcac cgtattcgtt tagagcttgc
     3301 actgtaactt tttttggtct ttttaattta ccccatactt ctggaaaact taaacatcct
     3361 tcaacaacaa tttgttcccc ttcttgttta ataatttcag gattaacaag cttaataagt
     3421 ccttctccta aatctataac aactaatcgc tttaatactc ctacttggca tgcagctaat
     3481 cctcctccat ttggagtatt gtacatggtc tctgccatgt catttaatat ttctcttatc
     3541 ttattatcta cgttttctac atacttactc ttttttctta aaatttcatc atcaaatgtt
     3601 cttatctttc ttatagccat aatattctcc cctacttatc tgtgctcttg attttctttc
     3661 actttcttca aataaaaata cactttcaat tggttcatta aaaattcttg aaatatcata
     3721 tgccaaccat attgaaggtt catttttacc agtttctata gcatttatag cctgtctaga
     3781 tgttccaact aatcttccta gttgttcttg tgtcaatcct aatttttctc ttaatatctt
     3841 tattttattt ttcatatttg ctccattcat gtaaggttaa ctttacatct taaatgtaac
     3901 atttttcaac attactgtca agtcaacctt acatttatat attaaatttt cttatttaac
     3961 tattttttta ctaaactttt taaataataa taaaatccat attaatgata taacaattga
     4021 aataaacata tagatataat tatcttcctt aaatgccttc gatatccaaa aagttggtaa
     4081 gaagaacaga atatattgtg ctttatttaa aataaaaaat ggtgctaaaa tacccatcat
     4141 aaaaacacct gagaatttcg taattaccat tccttctagt ttatttgatg ataaagaaat
     4201 cactagtatt gatataataa ctccttgtaa taaaactaat aaagaaattg ataagtttaa
     4261 caagaaagaa attttagtta gagaaaaaaa gataagtaga atcatagtaa taataaatgc
     4321 taaggtacct ggtattccaa atcgtgaaat aagataacca gttttaccaa gcggagttac
     4381 tattaaatat cttgaaatgc tgtcatcaat ttctcccaat atgaccattg tagaagcaaa
     4441 aaagaacata aaaggtgtaa taaaagctaa aagtaaatca aacatcaaga aatacggctc
     4501 taaatttaat tgataataaa acttgttaag tgatatattc tgtattaatg gtattccaaa
     4561 ttttataaat actccacata atattggaga aacacatact ataaaaagca tagcatctct
     4621 cttaatttgt tcaaactctt gtttaaacgc atttataatc tttatcatag ttttactcca
     4681 cctacctttt tccaactgct acaaataaaa taataagtta taaaataaat tattattata
     4741 attgatatta aaatattaat cattataaaa tttttatctc cactaataag agaaatacat
     4801 atattaaaag ggtacaaatt tgcatatgat tttgtgtcaa gaagtacatt gagtattggt
     4861 ggaataaagc atataatctc aataggaata cttaaaacaa taaactgatt caaactagat
     4921 gctttacttg caagtatcaa tccaagcaaa gaaaatataa tagaagaaaa aaaggtacct
     4981 atagttgata ttataatatt atctaaattt aatgtaatag caataaacat agctacaata
     5041 ctagatatta ctcctaatga aattaccttt gataaaatat actcagatac ttttactgga
     5101 gatacagcta aggaatttaa aaccctttga cttttctcaa gaagtacaat agctcccata
     5161 aaaaacaatc ccattgctgc tgggtcagaa taaatcataa ttatagctat tttttctctc
     5221 ataaaagttg gaaatacatt gataatacaa atatatataa tacttaaaaa aagatataaa
     5281 aaataaaaac catacttaaa ttgaaacttt atatctccta aaataagatt ttttagcctc
     5341 attgtaaatt cctccctgtt atctcaataa aaatatcatt taatgttggt tcactactat
     5401 gtattgataa aagtttattt ttcttaataa gcatattcaa atttttatca ttagcagtat
     5461 tgttcagaaa acattcagaa gttttttctc cattatcaaa gtaagtatat ctaactttaa
     5521 tagctccttt tgacataata agattatgtg gtgtatctaa agcagatatt ttaccattta
     5581 caatgaacgc tactctgtca caaagctccg ttgcatctaa catgttatga gttgttaaaa
     5641 taatagtttt acctttagat ttttctgata aaattatatc tttcataacc ttgctatttg
     5701 acgggtcaag accacttgtt ggttcatcta aaaataaaat atctggatta tgtaataaag
     5761 cttttataaa atttaatcga gatttcatac cttttgaata ctcagatacc cttttatttg
     5821 cttcattttc tagtcctact gattttaata actcatcaat agatagtagt tttttagaat
     5881 atagagaccc aaaatatttt aaattttcta ttgcagtcaa cttttcatat aaactaggaa
     5941 attcaaaatc aactcctata ttttcataaa atttatttga gtgtctttta ctctctactc
     6001 catttacaat gacacttcct ccataattag ttatcattcc aattagaatc ttttgtaatg
     6061 tagattttcc tgcacctgat ggtcctaaaa atcctagtat ttcccctttt tctacttcaa
     6121 agttaatatt ttgtaaaaaa tttctatctg tataactaaa tgataaatca tcaactttaa
     6181 tcattcaata tcaccctttc aaatttttga ctattttgac tatatggtca ataaaatata
     6241 atttttttgt ttgttaataa tttttaataa aagttattag caaacaaaat ttaaattcta
     6301 ttccataagt tgtataacta atccgtttat cattaatctt aaagcatctt caaaacaatt
     6361 acttccaacc tcgcgcttat atagcatggt aagaaaaact actctaaatg ctccactaaa
     6421 gctttctatg tctttatttt ttgcatttgg tatgcaagat ataatctctc caatttcaaa
     6481 gtcatcatgt ctaaagtgtt cttcaattat agaatctggt aactttctca ccaatatctc
     6541 aaattctcca tttatcatga gatttaaaat tgaagtacta tcaaccatcc tatagaattt
     6601 cataaatata tctgtcaatt gttcacatgt aatactttca tctactctat ttatctcata
     6661 taagatttct ttttgtatag agtcatgtat atctctcaat acctcaaaaa agagcaattc
     6721 ttttgattga aaaaatagat aaaaagttcc ttttggtatt tttacccgtt ttacaagctc
     6781 atcaacagta gtttttctaa taccatattg ctccatacac aatcttgctt cttctttaag
     6841 tctttttatt atatactctc tttctttatc agaatacgat ttaggcatct ttttcctcca
     6901 gtttgactaa aataatttat atagtcaata taatactttt tctatctaat gtcaatagta
     6961 ttattagtat atttaaaaat aagcttaaaa actttcgaaa attctgaatt ttatatagca
     7021 ttttttatta tctagtgtta taattaagat ataaataata tattattcta attttcatat
     7081 ctatttgctt attttttaac ttagtttcta atagaagtac tcatatttat ctttttaaaa
     7141 gtttcaactt taataaaaaa taaaggagtt gattttatgg ctactactac aaaaaaccgt
     7201 ttttacgatt atgttcaaga aaacttaaat aaatatgttg aagtacattc ttactttagc
     7261 agttgtcctc tatatggtga aattatagaa gcagatagct tatcaattac actttgctca
     7321 agatatatag atgaatctaa tccaacttta aatgaaactt acacattata tttaccatta
     7381 agttcaataa tatctattag aacactat
//
LOCUS       DAELSY010000172         7372 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.172, whole genome shotgun sequence.
ACCESSION   DAELSY010000172 DAELSY010000000
VERSION     DAELSY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.172"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(244..696)
                     /locus_tag="KPG09_003336"
     CDS             complement(244..696)
                     /locus_tag="KPG09_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0967900.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(1007..2452)
                     /locus_tag="KPG09_003337"
     CDS             complement(1007..2452)
                     /locus_tag="KPG09_003337"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBG0967901.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINKRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(2596..3504)
                     /locus_tag="KPG09_003338"
     CDS             complement(2596..3504)
                     /locus_tag="KPG09_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0967902.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            3761..4585
                     /locus_tag="KPG09_003339"
     CDS             3761..4585
                     /locus_tag="KPG09_003339"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG0967903.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILINEFIKLNF
                     SNTFINTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRVFFNSTVVAIALIVYGIIMIGLEKRNKRPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            4625..5155
                     /gene="lepB"
                     /locus_tag="KPG09_003340"
     CDS             4625..5155
                     /gene="lepB"
                     /locus_tag="KPG09_003340"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG0967904.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSVVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEILG
                     KVMVRLLPLDNIGKVD"
     gene            complement(5237..7018)
                     /locus_tag="KPG09_003341"
     CDS             complement(5237..7018)
                     /locus_tag="KPG09_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBG0967905.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
BASE COUNT         2427 a         1071 c          868 g         3006 t
ORIGIN      
        1 agaaatttaa aaagagatta gaaaataaaa ttattgaata taaaatggaa aaatataaat
       61 tacacaaaga ggtatgagta ggtactaaca aataagaaaa tgtataagaa attataaaaa
      121 agtacatcaa gaaaatttaa gatgtacttt ttagattgta tttagactta taattatata
      181 gttaaatgaa taacaaagaa ttaatcttag tactattaaa tataaaaatt ttttatattt
      241 tttctatcta aattcttctt tgtttaatat atattgaaga gtatttattg cttctaaata
      301 tttttttctg tcttcttcag ttaatttaga aagtttttct cctaaaccat cacttaattt
      361 atccataaaa tcagttgcta tcttatctcc ctcttcagta atgtcaatta gatattttct
      421 tctatcaact aaatctcttt tccttgttac atacttctta tcagttaaat catcaaccat
      481 actagttaaa ctgccttttt ctatatttaa cttagagcat aaatcagtca tacttattac
      541 tccataattt tttatgaaaa ctaatgctct taattgtgtt ttatttacat tataatctgg
      601 tgcaaatttt tttaggtctt ctaagtacag actagaataa atcttaggaa atgtttttac
      661 taaaaaactt atggtatctt ttactatata gttcatataa tcacctcggt tgttttaaaa
      721 tttattaaat tatctaaata actttatttc agtttacatt aaaaatattg caagtacaat
      781 agtttttgct gaatgtttaa atttaattag taatcttatc atttttttat ttttatttat
      841 attatttcta ttaaatatta tttatatagc tattttatta aattcatggt atagtaagta
      901 aagaagaact cttcacagcg ctgaagagaa gacgctagcc cattaaaatg gctatcttat
      961 ttataaataa gatagccgtt tttaattata ttttatctgt acaattctat actcttaggt
     1021 ccacatctac ttttcctaag tcttccttat aatcttttat agcaggttct acaacttcat
     1081 ttacaaattc tacaacttgt tctggagctc tacctataaa gttttttggg tccattatag
     1141 atagtatttc ttccttgctc attttaaatg aatcatcatt cattataagt tcaattaagt
     1201 tattatcctt accttcgtgc tttactctat aggcagcttt catagaatat tctcttatta
     1261 tttcatgtaa ttcctgtcta tctccacctc tttttacagc ttccataagt attgtttctg
     1321 tagccataaa aggaagttct tcatttacac gtttatttat catattttca tatactacta
     1381 aaccatcagt tacatttatc cctatttcaa gtatagcatc tgctgccata aatgcttgag
     1441 gtattgccaa tctcttattg gctgagtcat ctaatgttct ctctaaccat tgagtggctt
     1501 gtaccattgc agggcttata gattcagaga ttatatactt agataaagat gctattctct
     1561 cacttctcat tgggtttctc ttataagcca ttgctgatga acctatttga tttttttcaa
     1621 atggctcttc taattctttt aaactttgta aaagtcttat atcgttagtc atcttatgca
     1681 tactttgagc tatccctgat agtatgctta ttacttgata atctaacttt ctagtatatg
     1741 tctgtccact tactgcatag gaagaatggt atcccatctt tttacaaatt ttcttatcta
     1801 actcttttac tttttcgtgg tctccttcaa ataagcttaa aaaacttgct tgagtaccag
     1861 tagtaccttt aactcctctt aacttcatgt tatctattct atagtttaaa tcttccaaat
     1921 caagaattaa atcttgagcc cataaacaag ctctttttcc tactgttgta agttgagcag
     1981 cttggaagtg tgtaaatcca agagctggca tatctttgta cttaagtgca aattctttta
     2041 aattgtttat taaatttaca agctttactc ttagaagttt taaagcttca ttcatttgta
     2101 taacatctgt attatctcca acatatgcag atgttgctcc taaatgtatt attcctttgg
     2161 ctttttcaca ttgtaaacca taagctttta cgtgagacat tacatcatgt cttacttctt
     2221 tttctatttt tctagcttca tcaaaattta tattatctat attagctctt agctcatcta
     2281 attgttcttc agttatattg attcctaact catgttcacc ttctgcaagt gcaacccata
     2341 accttctcca tgtagagaat ttaaattgtg gagaaaatat ataactcatg tccttgctac
     2401 aatatctgtc tgtaagtggg tttgagtaag tagtatattt attatctatc attatattag
     2461 caccttcctt aaatcttttg tcgtgtgatt actgtgtaca cattagtaca taaataatta
     2521 tatataattt tacgaactat aacaagactt ttttattttt tgttcacatc tatatatagt
     2581 attttttatt tattactatt ttttatttat atccaaatta tctttttcaa ttaagtaatc
     2641 cactatttca ctatcaacaa aatttaatac atacttatat ctaattgcat agtatttatc
     2701 actccatcca agctgatttt ttacatcact ttcatctgcc ccaagcctta aacttattgc
     2761 tgcaaatgaa tgtctaaaat ctcttgctga atattgagaa agtccagcta aatctaatgc
     2821 ttttttgact ataagtctga catttctatc tgttatagaa ttactctttt gagttgtaaa
     2881 aataaaatca tcactaggta ttattacctc tggaagccca gaataattcc tgtaatcttc
     2941 taaaagttta aaaaagtatg gatgtaattt aactactctt tcttttcttc ccttacctaa
     3001 ccttacatat gagttatcgt tttcatctac cataaggtct ttccacttta agctaactaa
     3061 ttcatttaat aaacacccag tagtaactag acagactatt attactctat ctcttatatt
     3121 taattttggt aggataccta taagtttatt tatttcttgt atacttagaa catcatcttt
     3181 tgtttttatt cttgttactg tgggtttttc tacatatcta aatggattta tatctatgta
     3241 tccttctttt tttaagaagt tataaaaact atgtaaataa ctatatatct tttcacaagt
     3301 tgatttcgca tatttagtct gtatatagtc tacaaaattt ttacaatctt cttttgtagc
     3361 ataaatcaat tctttccctt ttaaaaattc tttaaacagt ataacctttg ataaataatc
     3421 gtgtttcgtg tttggattta attttgtaga aaaaacatta aaagtttcat actcataatc
     3481 atctagtatt tttgtactat acataataac tcctttcctt ttcataaaat aaaatagcta
     3541 tacttttact ataccatata tttccccctc aaatataccc cttattcaat agataacttt
     3601 taagtttatt ttgttatatt atgaaggttt tatgaaaata tcaatcttcc attgtgcact
     3661 acacagtact atggtattat aagttagtaa aaggtggcat ttttttatta atctatgaat
     3721 aaaatatgct actataaatt tgtattggag gtattttgat ttggaaacat ttagtttgct
     3781 tgaaattttt aaggcagtca ttttgggcat agttcaggga ataacagaat ggctaccagt
     3841 aagtagtaca gggcatatga tattaattaa tgagtttata aaattgaatt tttcaaatac
     3901 atttataaat acatttttag ttgtaattca atttggttca atactagctg tacttgttat
     3961 attttttaga aagttaaatc cttttgatag tgcaaagaat attaaacaga aaaaagaaac
     4021 agttcgttta tggcttaagg ttataattgc agttatacca tctggagtta ttggaatact
     4081 gtttgaagat gacatagaca gagtattctt taattcaaca gtagttgcta ttgctcttat
     4141 tgtctatggt ataataatga tagggcttga aaagagaaat aaaagaccta agtataaaga
     4201 tttttctcaa gttacatata aattagctct ttgtatagga ttattccagt gtctagctct
     4261 cataccaggt acatctaggt ctggttctac tataataggt gcagtattac ttggtacttc
     4321 tagatatgta gcagcagagt tttcattctt cttagccatt cctacaatgc ttggagctag
     4381 tgcattaaaa cttctaaaag ctggatttgg atttactgga tttgaatggc ttattttagg
     4441 tgttggttct gtagtagcat ttgttgtttc aattgtagtt attaaatttt ttatggacta
     4501 tatcaaaaaa catgacttca aagtatttgg ttattataga atagtcttgg gtatagtggt
     4561 tcttgcttat ttctttctat tataatgtat tctaatttaa taacaaatga taaggagaaa
     4621 agttatgagt gttaaaaaag aaatatttga ttggattaag tcaatagcta tggctattgt
     4681 acttgcattt gtaattctac aatttataag accttctgtt gtaagtggag aatcaatgta
     4741 tcctacttta gatgataaag attatctgat tttaaatagg atatcataca aggttggtaa
     4801 acctgaaaaa ggcgatattg tagtttttaa aaccaattta gttgatggag aaacaggaaa
     4861 gaaaaaagac ttaataaaaa gagttatagc tactgaaggt gacagaataa aaatatcaaa
     4921 ttctaaagtg tatgtaaatg gaaaattatt aaatgaacca tatatacaca ataactatac
     4981 ttctggagat atagatactg ttgttccaaa aggtaaacta tttgcaatgg gagataatag
     5041 agaaaatagt aatgatagta gattccctga tgtaggtatg gttgatgaag atgaaattct
     5101 tggtaaggtt atggtgagac tattacctct tgataatatt gggaaagtgg actaaaaaat
     5161 aagagactga attggtaaag tttattttac aaatctcagt ctctttttat tttcttaaat
     5221 tacaaatatt tttttactaa atatcttttg attctgtaat aagtcttatt atatcacttg
     5281 cttcttcatt aaaagttgtt actatactta ggtagtctgt tgctctactc atagctaaat
     5341 aaaccttatt taaatctcct acaaagtcat ttacttgata atcttgttca atatcattta
     5401 tagtttgatt gtataacatc tctaattcac atactattac cgctttatac tctaaattct
     5461 ttattgcata tatatttgct attgtagttc ctaccttctt acttatattt gtcaaattat
     5521 cttctgcaca tatataagga atttttgcct cttccaatgc tttttttaac atatattgaa
     5581 agtaaatggt ttttccactt tttaatttct tcttattata aggataaacg atagcaactt
     5641 cagaataatc aagacctttt ttacttatta aatactcaat ttcccagatt acagaactta
     5701 tttggtcatc aaggtcactt acttttatta tatcaacaga cttatttcca cctctaagtg
     5761 ctttagtata aaagaaagta ctaaattctg tatttggtct taattcgttg atatatgagt
     5821 tagaattatt tgaaaattta tttgtaaaat ctacaatctc tttagcttgt ctataatttt
     5881 tacttaagat aatcttatca tcaaattcta taccattata taatttttta aatatattca
     5941 atctattaga gatattcaag gagttacaag aacacacatt aaatatgaat tttgttttat
     6001 ataaaaactc ccttataaaa tctatttcat actctaaaaa gctttctgct tcatcaataa
     6061 aaatagcctt gaacatattt ttatttttta taatgttctg tgcttgtttt acaagattat
     6121 taaaggtctt ttcgtaatca ttttttaaca tgttataatc tacaattaaa ttgaattttt
     6181 tagctagttt aaatataaaa gaactaaatg tgtgaacttc taaattgtta ttatctttgt
     6241 ataataactc tatcctctct cgaagctcat tgcacaattg ttttgtatgt gtaaatatga
     6301 ctaatttatg atgtggatat acttttgcta attttatagc cctagaaagc ataattgctg
     6361 ttttgcctat accagaacca ccttcaataa gtgtattccc atatttaata gaaacaacat
     6421 ctttaatttg aacgtcttcc atcatagttg ctgtatactt atagtcatca ttgtagaatg
     6481 aaattttttt aaatttttca tttagatgaa gtttatcatt tattatatag tattctgaac
     6541 atatgtctag taaaaataaa tttaagttta tttcatcatt ctcatcatta agatactcct
     6601 ccagcgaacc cttattcatt atatcttgaa gcttattttt atctattata ttattattaa
     6661 caaattcttc aaattcatat gtttcctcaa cttcaacgta tggcataatg taaacgtagt
     6721 tataacttat atttctattc ttttgtccca tttttagttt caaaagttca tattcctcat
     6781 tcataacttc aagtaactct tcttctaaaa taaaaaatat atcttctgtt gtatccataa
     6841 attttataaa taaaattctt ccatctttaa catacaacaa atcggtattt attcctttaa
     6901 aaggagtaac ttttggcact actttaacgt cctcattgat attttcgtaa aatattttct
     6961 cgaactcctt caattttagg gcgtctaact gagaatttat atttatgtat tggctcactt
     7021 acattacctc cttctgttat acttaattat accattttga actatatttt tatatatact
     7081 ttttataggc aaatttagta aataaattca aaataaatac aaataaatac aaaactgcta
     7141 aaataatttt gcaataaata tagatattta ttgttataga atactttctg taaataataa
     7201 aatgataaat ttcataatta atgtttaaaa tagtaagtaa agttttaaaa aaaataaatc
     7261 ttgttgacta aaaataagat ttattataaa aaatttaaaa acctgctaaa aataatctat
     7321 tttcattagc aggttttatt tataatcttc ttatattaat actattatat cc
//
LOCUS       DAELSY010000173         7297 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.173, whole genome shotgun sequence.
ACCESSION   DAELSY010000173 DAELSY010000000
VERSION     DAELSY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.173"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            145..1548
                     /locus_tag="KPG09_003342"
     CDS             145..1548
                     /locus_tag="KPG09_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0967906.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIIFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLTLANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGSK
                     FVGGKSIVFSIIGIIFLIILISGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNKYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            1768..4245
                     /locus_tag="KPG09_003343"
     CDS             1768..4245
                     /locus_tag="KPG09_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0967907.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDVLTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEMTENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIENVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDVVMIQIRDLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKKRSIAMYKSIGLSDK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            4260..4946
                     /locus_tag="KPG09_003344"
     CDS             4260..4946
                     /locus_tag="KPG09_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967908.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALVGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            5219..6052
                     /locus_tag="KPG09_003345"
     CDS             5219..6052
                     /locus_tag="KPG09_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0967909.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCMQLKNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMYICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            6090..6551
                     /locus_tag="KPG09_003346"
     CDS             6090..6551
                     /locus_tag="KPG09_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG0967910.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            6704..7129
                     /locus_tag="KPG09_003347"
     CDS             6704..7129
                     /locus_tag="KPG09_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0967911.1"
                     /translation="MHDELKIKMYSFTMDCKDPHELAKFYAALLNWEIAFYNEEYACV
                     GAPGTNQGTYPGITFQRNSEYKPPVWPEKPESQQQMAHMDFAVNDLEEAVQYEIHCGA
                     TIAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
BASE COUNT         2820 a          792 c         1134 g         2551 t
ORIGIN      
        1 cagcttgata acatataatt atataaatat aatttatgta gatagttgta tttttaaata
       61 aatttttaca tatcaataat tattatctat tttatataag caacaatcaa actaaaataa
      121 aagtttaaga aaagagttga ttaaatgcat aaacctagtt ataaaacagt atttcattca
      181 tacattattt ttttcatgat ttttatttgc ttaatcctga cagtaatagg tattttctta
      241 tcacttatta ctattcagaa accaaatggg gaatttattc gtagtgactg gccgaaaaaa
      301 tttacagaaa actttaatga acaaatcata tttatagata ataagccaca agtaaaacag
      361 tctggattag aaaagcttcg agaaaatcaa ttgtggttgc aaattttaga ttctagtgga
      421 aatagagttt atgggttttt agaaccaaaa gagcataaag attattattc taattctgaa
      481 ttattaacat tagctaacca aaataattca agtaatggaa ctgtatactt taatacaatt
      541 aaaaataata aaaatgacta tatttatatt atatattttc cattaaatat atctaaagtg
      601 aatatgtttt ttgatggaag taaatttgta ggtggtaagt caattgtatt ttcaatcata
      661 gggattatat ttttaataat tttgatttct ggaattgctt atggttactg ggtaacaaag
      721 tgcatatcaa atattacatc tgctgttagt gatatttcaa aacgaagcta tcttccagtt
      781 aatattaaag gtgtttttgc tgatatatat aatagtttaa atattttaaa tgaagaaatt
      841 catagaagtg atgagataca aaataaaact gatattatga gagaagagtg gatttcaaat
      901 ataactcatg atttaaaaac tccactttca ccaataaaag ggtatgcaga gcttcttaca
      961 gatagtagta ctaattattc acctgaaaaa gtatataaat atgcttcaat catgttaaag
     1021 aatatagaat atacaaatac acttataaac gatttaaaat taacctatca attaaaaaat
     1081 ggaataattc ctttaaataa aaataaatat gatttagtaa gattcttgaa agaattatcc
     1141 attgatattt taaattatcc agaatatgaa tcaaatatag ttgaatttaa gagtaatata
     1201 gataatatta actttgattt tgattgtaca ctattaaaac gagcttttag taatcttatt
     1261 gtcaatgctt ttacatatgg agataaaaat actaaagttt gtgtacatat aacattaaat
     1321 gaaagaattg aaatcattat atctgataat ggaaaaggga tgtcaagaga agaagcatca
     1381 aatttgttta atcgttatta tagaggaagc aatacaaaac aaaaaataga aggaactggt
     1441 ttaggactag caataacgaa gcaaattatt gaagttcatg gagggaatat atctgttgaa
     1501 agtatacctg gattaggtag ctcattttat atcacctttc ctatttaatt aaggttaatt
     1561 aaggtcaaat aaaaaatata ttaaggttga tagattatta tatttatata gtagtttatc
     1621 aatctttttt atttaaggag gtgatttgat taaaaatatt ataaatagaa tgataaaagt
     1681 atggaggagt ctattagaga ttcaatttgg attaaagtat aaaaatatta tactgaaata
     1741 tcttagataa aggaagaagg tgtaaatttg aaaattatat taaagtatat tttcacaaat
     1801 attaaagaac gaaaaattag aacagcagtt atgttactgt ccatagtttt atcaacagtt
     1861 ttactttttg tttcattctc aattggatta tcttatgaaa gtgcacaaag aaaaatggca
     1921 aaaggtatgt atggtacagc gactatatct gtccaaagta aaaatccaga tgtattaacc
     1981 aatttagaag acatacctga tttaaatgct ataaaatcaa aggttggtgt attggaaagc
     2041 tctgctattt ataataaagg gggatactat gaagaattta gtcttatatc agcagactta
     2101 agtcaactaa ataaaatcaa caagccacga ttagaaaatg gcgatagtat tactgatttt
     2161 tctggtgata aaattattct tccaaatcgc tttacatcaa aatataaaat aaaaaaaggt
     2221 gattctatta ctttacagat ttatggaaag tcttatactt ttcaagttag tgatattgca
     2281 tcttatgata cagtattttt gaggaataca agaggtgtaa atgcactgct acccaaagag
     2341 actctatcaa aaattataaa taaaggcagt ggatatacaa gagttcttat agagtcagaa
     2401 gaagaaatga cagaaaatct agtaaataaa ttatccgaag aactttccac tgaaaaatat
     2461 atggtatcaa atactataaa tgaaactaag ataatttcag atgctagaca aaagacaatg
     2521 cctttctttt taataagttt ttttgcatta acccttagta tatttatcat atacagcagc
     2581 tataaggtta ttacattaga acgtcttcct tttattggaa catttagaag tattggtgcc
     2641 aatgaaaaaa ctgtaactcg tatattgatg cttgaaagta tattatacgg aagtatagga
     2701 ggattaattg ctattcctat tggtgtagtt gttttaaatt taatgcttca tgggttagga
     2761 aattctctcg aacaaggaat atcaattcca gtagtaatat cacccattgg agtgattata
     2821 tctgttatag tagcaataat cgtttcttca tttagtgcat atatacctgt taaaaaagct
     2881 agccatttgc caataaaaaa tattgtacta ggaacagtag aagaaaagaa tgtttcaaat
     2941 cgttctatac tttttatagg aagtataatg tttattttat ctattttact tccaagaata
     3001 tcccctgaaa atacattgta tctagctggt ggattttcat tactaggatt aattgtagct
     3061 actattgtgt tgattcctct tattactgat atcatgtcta tagtttttga gtttgtttat
     3121 aaaaatattt tagggaatga gggtaaatta gctgctagaa atatgaaaaa taacaaaaat
     3181 ataatacaga atatcacttt actatttata agtatttctg cagttattgc aataagtgtt
     3241 gttggtaact ttgtcaaaac ttatataaca gatgtgtttc gtgacgcaga attacaaggt
     3301 tttgcagatg gaaagatgaa tgaagagttt attgaaaatg ttagacatat ggatggaatt
     3361 aaaaaaatat tacctcttta tgttatgaat aatgagattt ctggtaatgg tgtaacatta
     3421 tcaagactag agggtacaga taatataaaa ctatataact ctatgtttgg aatcaattat
     3481 acaaattttg agataaaaaa acaagtaata gaagctttta atgataaacg ttctgttatt
     3541 ttgaatgaag atactctaaa aaaagtaggc ttatctatag gagatacaat aactctatct
     3601 aatgataagt atgatttttc ttataaaata gtaggtagtt ttaaatcccg tgcaaatgat
     3661 gtagaagctg ttataccatc acattatgca gttaatgatt ttgataaaac taattatggt
     3721 tttttagtat atacagcagt caatccagat gttgttatga ttcagataag agatttattt
     3781 ggagatacat ataattggag tcgtacagtt gaagaattta ataatgattc tttaaatact
     3841 ataagtagtt ttttgtctcc tatgaataag atgacctatt tcatttttct tcttgctaca
     3901 gtaggtatta ttaataatct actgattaat tatattcaaa aaaaacgttc tatagctatg
     3961 tataaatcta taggactaag tgataaacaa aatataaaag tgacattgat tgaagggttt
     4021 acttcaggtt tacttggagc tgttattggt attgtgatat ctatacttga gattcaaact
     4081 atctttatag ttgcaggacc aaaaatttca atgaaaccag atttagattt taaaacattt
     4141 attattgttg ggttattggg aattatagta actttaatag gttcgatagt gcctattata
     4201 aaagggaaga aaatgaaact gatagaagaa attaagtttg aataattatg gaggttaata
     4261 tgaaaattac acataatagt ttagctgtag aagcaaaaaa tattatcaaa gaatataaaa
     4321 ttggaaatac aacaactcga gtgttaaaag aggtatcttt acaagtaatg aaaggtgaat
     4381 ttgtttctat catgggtcaa tctggttctg gtaaaagtac tcttttatat attttaggtg
     4441 gtttagatac tccaacgagt ggaaaagtgt atatgaatgg agctgatata tcacatttta
     4501 atgatgagaa aatgagcata atacgtcgta gaaatatagg attcgtattt caattttata
     4561 acttaatacc aaatttaaat gtggaggaaa atataatgct tcctttgtta ttagatggta
     4621 aaaatttaaa agattataaa aatcaattag atgaaatctt agatatagtt ggtttaactg
     4681 atagacgtaa acatacacca cgagagttat ctggtggtca gcaacaacgt gttgctatag
     4741 ctcgagcttt agttggaaaa ccagagattt tatttgctga tgagcctact ggaaatttag
     4801 atagtaaaac agggatagaa ataatagatt tactgaataa aataaacaga gacaatggac
     4861 aaactataat tatggtaact cactcaccag aagcagccaa aagtagtagt cgtactatta
     4921 cagtttcaga tggtttaatt gtataaggac taattattgt atttgagata agcattttta
     4981 actatataaa ttagacatat gtaaagtgga tataagttaa atactataaa tgtgctataa
     5041 tattaaaata acagataaaa gtactatatt ttttattgat tattttaatt tatttgttta
     5101 ttaaatatct aatgagtatt catactatct ctattattaa aagcagttaa tgatatatag
     5161 agataatatg gattcttctt actttaataa atattcacga taggacagga tataatatat
     5221 gttaaaaaaa gaaagtattc attttataaa taaaaatagg tcacttatag tttgtgaaga
     5281 caattttgtt tatctcttgc cttgtatgca attaaaaaat tggatatcga attttactat
     5341 atcgtttcca aatcaaacaa taatttctga taattacacg attattcctc atggaagtgt
     5401 aactttggtg tttttttatg atgccacagg gttacatagt ctattatttg gtccaactac
     5461 taaaccaaaa acagtaggaa atattgctaa tagatgtgat gttatactaa ttatagaatt
     5521 tcaacctgct ggttttttcc cgcttatagg tatacagcaa agtgaactta ttgataaagt
     5581 tgttccattt tctataatta atacttcatt agatttagaa attaaaaaaa tatttaatga
     5641 atctttatct atagataaat tgattttaaa gttggaagaa ttattgattt ctaatattaa
     5701 aattgaatac tcatatgagt ttattttggc aattcagttg attattcaaa actctggaaa
     5761 catatcttca caagaaattt ctaaaaaagt attttatagt tctaggcatt taaacagact
     5821 ctttaatcag tacctagggt tgagtatgaa gtcattttct cgtttggtac gtattaataa
     5881 atctataaaa cttctaaaca ataataaaac ttcattgatg tatatatgta atgaattagg
     5941 attctatgat ataccacatt ttattaaaga ttttaaaatt gtctgtggaa taactcctca
     6001 aaaataccga gctaatatgt ctgattttta cagtgaaatt gcaaaatttt aagttataat
     6061 acttataaca aagaaaggat agtgataaga tgaaatttgg aggagtattg ctaattgtca
     6121 aagacataaa taaatcaaaa aagttttatg aagatgttat ggagcaaaaa attacattag
     6181 atttaggaga acatgtatca tttgaaaatg gatttggatt acaaagtaat tataaagcta
     6241 taattggata tgatttacca atatatcaac aatcaaataa ctttcagttg tattttgagg
     6301 ttaatgatat caaacaatgg gaaagtaaga taaatagaat aggaaatatt gaatttttgc
     6361 attatataaa agaataccca tggggtcaaa gaacttttag gttttatgat tttgataaaa
     6421 atattataga aatttcagaa agtatggaaa gcgtcataaa gaggctttta aaacaaggtt
     6481 tagctttaga agaaatttca aaacgcacta tgtatccagt tgaatttatt acacaatttc
     6541 aacaattata aaatattaaa atatataatt ttttctttat attgcaaaaa acaaaggctt
     6601 ggggcataat agaaaaatac ttgaaataaa ttacatactt aatgtttgaa tttagcttaa
     6661 aaatgaaaag acgaagaaat ttatacttgg aggtaattac gttatgcatg atgaactgaa
     6721 aatcaaaatg tactcattta cgatggattg caaagaccca catgaattag caaaatttta
     6781 tgcagcgtta ctcaattggg aaatagcatt ttataatgaa gaatatgcat gtgttggtgc
     6841 tccaggaaca aatcagggaa catatcctgg tataacattt caacgaaatt ctgagtataa
     6901 accgcctgtg tggccagaaa aacctgaatc tcaacagcaa atggctcata tggatttcgc
     6961 tgttaatgat ttagaagaag cagttcaata tgaaattcat tgtggagcta caatcgcaga
     7021 ggagcaattt actgatgact ggagagttat gattgaccca gctggacacc ctttttgctt
     7081 atgtcagatg aaatcaatta tggaaagtag ccatttttca ttgttataga ccaaaatatt
     7141 gtttattaat tacaaaaaag cacaacagta tataccgttg tgcttttact tattatacat
     7201 gaatacacta aaaaatataa atagtagagc tttatcaata atgataattt ttaaaaaata
     7261 gtattttttt attgaataat ttcttagata ttttaaa
//
LOCUS       DAELSY010000174         7267 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.174, whole genome shotgun sequence.
ACCESSION   DAELSY010000174 DAELSY010000000
VERSION     DAELSY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.174"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..240)
                     /locus_tag="KPG09_003348"
     CDS             complement(<1..240)
                     /locus_tag="KPG09_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967912.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKE"
     gene            complement(347..850)
                     /locus_tag="KPG09_003349"
     CDS             complement(347..850)
                     /locus_tag="KPG09_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0967913.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            complement(902..2101)
                     /locus_tag="KPG09_003350"
     CDS             complement(902..2101)
                     /locus_tag="KPG09_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBG0967914.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKNIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEENKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            complement(2361..2549)
                     /locus_tag="KPG09_003351"
     CDS             complement(2361..2549)
                     /locus_tag="KPG09_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967915.1"
                     /translation="MNYHTKEELMEVLRVASSRIINCEKVQKKFSEETSHHTRFKNII
                     EAMYISKSLIMDEISKRD"
     gene            2884..3852
                     /locus_tag="KPG09_003352"
     CDS             2884..3852
                     /locus_tag="KPG09_003352"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBG0967916.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTKL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPNTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLRECIALSKTL"
     gene            complement(3969..5099)
                     /locus_tag="KPG09_003353"
     CDS             complement(3969..5099)
                     /locus_tag="KPG09_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0967917.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDINISDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(5252..6205)
                     /gene="msrA"
                     /locus_tag="KPG09_003354"
     CDS             complement(5252..6205)
                     /gene="msrA"
                     /locus_tag="KPG09_003354"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBG0967918.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRIEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEVLGYGEYLKLFE"
     gene            complement(6329..7234)
                     /locus_tag="KPG09_003355"
     CDS             complement(6329..7234)
                     /locus_tag="KPG09_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG0967919.1"
                     /translation="MKSSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
BASE COUNT         2410 a         1254 c          704 g         2899 t
ORIGIN      
        1 ttctttcaac ataactcctg caactccaaa tataaaaaat agagaagcaa taaattttcc
       61 aataagatta ttattaggat aataactaaa aagcataaaa caaagtacaa atgtaagtac
      121 tcctattgca ttaaacacaa ttttattaga tgctattttt cctttattga aaaagaaaca
      181 gaatattgac actaaatcta aaaataaaaa tacataaaac attcctttta aaacgcccat
      241 ttttataccc cctaaaaatt ctaattataa tatccagttt atttcatata atcgtcaata
      301 taatcaatac tttataacat cagtataaca gtattacaaa tttatattac gaatgtatta
      361 cttcgtattc atacatatta taatcacact catacatatt taaagactca tatgttgctt
      421 ttgcatttat gttttctttt tctacatata atctcattcc aactatattt tcatctttat
      481 cacatatatt ttttatataa ttaaataaat aattaaagat accttttctt ctatattcct
      541 tatctacata tacactctga atccaaagga aatttccatt tctccaatca ctccactcat
      601 atgtatacat aatctgtgca acaactttat caaatactgt atatacatga tattttcctt
      661 ttctctcatc taataacaat gcttttacac ctttagttaa aacatccata tcaagttctt
      721 ttccttcagt ttctttagcc aaattataat taaattttgc tattgtatcc aaatcatcca
      781 tacgtgcttc tcttatctct attagttctt cattttcata taactctatg tagtcctttt
      841 gttgctccat acttattcct ccatatctat tttatctact aaggtttttt atataatata
      901 tttatagagt tttttctatc ttcataaatg cataatcaag tgtagcctct aaatttccat
      961 tattcttacc tcctccttga gctaatactt tacttccacc acccttgcca tctataagac
     1021 ttattgcatc ctttaataaa actcccatat taatatcttt taaattttta gaacatgcaa
     1081 aaactaaatt tattttatca ttaaatttaa cagctaataa tgcaatacta ttttcatatt
     1141 ctgcaatttt atttgctatc ttatttacat atttcaaatc ttcatcatca taaatttttt
     1201 taatgaccga aatattgcca accttatttg ctttttcaat catttctttg acctgatatg
     1261 tagatactac ttcttcaagc cttctttttt taattaaagc ctcttccact ttactattta
     1321 gatttacaat accatttata gcatcttcct cactacagct taaatatcta catattttag
     1381 tcaaatatat atccctcttc aatacttcat ccactgccct tttacctgct agaaactcta
     1441 ttcttgtact tgacttatgt ttttcccatt tctttatttt tatcattctc aattcaccag
     1501 ttgattttgg atgcactcca caacatgcat tcgtatctaa atcacctatt tttattactc
     1561 ttatctcttc atcagtattt ggcaaatcac gtcttaacca aatctttttt agttcttttt
     1621 tcgttggaag taatgtctct acttgtatat tttggcgaat aatttcattg gcaaacaact
     1681 ctatctctct aattttgttc tcttctaaat taccttctat atctactgta ctatactcat
     1741 ttcctaaatg aaaacctgtc gtatttgcat taaaagtttt aaaaaaacat ccagataata
     1801 catgttgtcc aaaatgttga tgcattccat cttctcttct atcccaatca atactacact
     1861 ttaccttatg tatctttata ggttttttat ctaatacatg gtatatattt tttccatctt
     1921 catacacatt tattacatca tcattctcta tttttcccag gtctccaaac tgacctcctc
     1981 caccaggaaa aaaagctgtc ttatctaata aaacatgata cttattttct acttctttta
     2041 tttcaattat ttctgctgta aaatctctta tatactgatt tgtataatat aacttttcca
     2101 ttataattct atctccattt tcttaattat agactacttt actttttaaa tacaaatttc
     2161 aagctaagct taatttaatt gtattaaaat agaactcatc ctattattta ctaacatctt
     2221 ctactatatt ttaacatata gatattagtg ttaacaaatt tataatttta tattatttca
     2281 atattttata acaagtatgc ttacttattt tcttatataa taaaatgtat attaaaacat
     2341 attaaattgc aaaataagct tcaatctctt ttactaattt catccattat taatgatttt
     2401 gaaatataca ttgcttctat aatattctta aaacgtgtat gatgagatgt ctcctcagaa
     2461 aatttcttct gtactttttc acaattaata atccttgaag atgctactct cagtacttcc
     2521 atcaactctt cttttgtatg ataattcata acacatttac tcctttgttt tttaatttag
     2581 agaaacagta tacctcaatt taatatcatt atcaactgat atcattatta tttatatata
     2641 tcttttaaca cttaaatatt ctaactatca gtgacattac atattttaat aattattaat
     2701 atacatataa tactttaaca ttcattaata gagagtatat taacattgct tttttaaaaa
     2761 ttttagtata aaatcatatt aaataaattt tctgaaataa taaaatatta aaatatccaa
     2821 aaacagcttt tgataacatc tataattttc aatgtatcgt tattttaggg ggagtgattt
     2881 aaaatggcaa taaagccaag aaaaatatct attgtaggtt cagggcatgt tggttctcat
     2941 tgtggattta gtttaataac tcaaggagtt tgtgatgaac tctttatgat tgatatagat
     3001 gaatctaaat caaaagctca agctttagat ttagctgatg cagtttcata tctaccacat
     3061 aaagtacata tagaaaaagg aactttttct gactgtaaag attctgacat agtagtgata
     3121 agtgttgcag actcttcaga agggccttta cgtagacaaa acacaactag attagattta
     3181 ctaagaccaa ctattggaat gataaaatct atagtaaaac ccatagttga ttctggcttt
     3241 gatggtatat ttgtagtcat atctaatcct gttgatgtag ttacaaatta tatttgggaa
     3301 aaatctggct ttcctaaaaa taaagtcatt ggaactggaa cagctttaga ttcaacaaaa
     3361 cttagaagga ttttatctga agaaactgga atcgctcaac aatcaattca agcttattct
     3421 atgggtgaac atggagattc tcaaatggtt ccttggtctc atgtttcaat tggtggtaaa
     3481 ccaatcttgg acatgataaa agacaaccca aatacttata gcaatttaga cttaccatca
     3541 atagttgaaa aaaataaaaa aacagggata agtataataa atggtaaaga ttgtacagaa
     3601 tttggtatag gtactgcttt agtagaaatt gtaaaagcta tactacacaa tgagaaaaaa
     3661 gttcttccag tttctacatt actagaaggt caatataacg aaagaaatgt atttgcaggt
     3721 gttccatgtg ttataggaaa agatggtatt gaagaaatta ttgaaatcaa tatgactgaa
     3781 tatgagcaaa acgaatttaa taaatcttgc tctgttttaa gagaatgtat tgcactttca
     3841 aaaacacttt agattcaata ttctttctta cactaaaagc aggtaaatga cttattgaaa
     3901 agacttttaa ctaagttaat gtttaaatca tttatacctg cttttaattt taatactaaa
     3961 atataaacct attttttacc atcaaatttt ataactttac tttttttatt tacctcttga
     4021 gataacaatc cttttttcaa tcccatcaat aattctaagt cttctggata aagatttatt
     4081 ccaaattcct ctaaacttga tactatacta tttacactca ttattttatt ttctatcaat
     4141 aattcaactg ctctttttaa atacgttggg cttgaacctt tcattttgtc tagtggttct
     4201 tctatcaacc agcctttttt atccatttca ttcataagat aattgtattt tttgtaattt
     4261 atcttttcta gattatatgc tctaaaaatc atagcagata taggtacaat ccattttttc
     4321 tttagttcta cataataatc taaatcttct ggatgagtca aatcttttaa aaaactctct
     4381 tttggcaata aaaatgcaca ggcatactca tcttctgaaa attgcttact tggtattctt
     4441 aattcacttg atattatata tccaagctca catgccaaag tataatttct aattgatgct
     4501 gactttttgt cattacccag agatattaga tatttagatt ctttactaat tctctgtttt
     4561 tgagtaaaga tagttgcccc ttttctatct acattcatac ctgatattat aatcccttta
     4621 gattctaaca aacttaccat atttactatt ggagtactat ttaaatccca aaactctcta
     4681 gtcttatatg caagagattc tatatcactt atattaatat ctgacattac ttttctattt
     4741 ggcaagtttt cttttggaaa cttaatataa ttctccataa atgaatatat tttatgtatt
     4801 ataattattt tttctctata agaaatctct tctactctag gtagtttaga ttgagggtta
     4861 aaatggctat cctctacaac gatatttata ttttcatttt tataaaaata ctcttttgga
     4921 aaatttaata tattagataa ttttaatgta ttttctatat ttggtacata cttattttcc
     4981 tcaaaagctt ttaaatcttt tttatttatt ttagtttctt tagacaactg ttctatcgtc
     5041 tttgctctaa aaattctagc tgattttaat ctctttccat taaagtcttt taaattcatt
     5101 tgttttctct ctttcaaaat taaattctta atctattggt ataaataaga ttacaattaa
     5161 taatttatca tattattgac aaaaaaacaa aaggtgaaaa tattttttta tattttcacc
     5221 ttttagataa gtttataata aatattccta attactcaaa aagttttaaa tattctccat
     5281 atcctaaaac ttccatattt tctcttggta taaatttaag agaagcagaa tttatacaat
     5341 atcttaaacc tccaagttct tttggaccat catcaaatac atgacctaaa tgagaatttc
     5401 ctttagcact tcttacttca attctgtaca taccttgtga gaaatcactt ctctcaatga
     5461 tagatttatc tataactggc tttgaaaaac ttggccaccc acatccagaa tcaaatttat
     5521 cttttgaaga aaataacact tctccactta caatatcaac ataaattccc tctttgttat
     5581 tatcataata ttcattttca aaaggtactt ctgtcatatc attttgagtg acttcaaatt
     5641 gtataggagt taaagtcttt tttaactctt ccctatttat attgtttcca tcccaatgac
     5701 tttttacaaa attatatctt ccagaccctt tataatacat attgtagttt aatggtcttt
     5761 ttttataata atcctgatga ttttcttctg cttcgtagaa tttagttgct ggaattatat
     5821 cagtcactat tttactatca aatattttac tattttccag atgttctctt gactctaaag
     5881 ctatattttt ttgttcttca tcatgataaa atatagcagt cttatattga gttcccctat
     5941 caccaaattg tccaccatca tctgtaggat taatctgttt ccagaagata tttagaagtt
     6001 catcataact tattatatca tcatcatatg ttatttgaat tgcttctaaa tgaccagtag
     6061 ttcctgaaca tacctcttga tatgtagggt tttcaacatg accacctgta tatccagaaa
     6121 ctactttaat aactccatca tacttatcga aaggttttac catacaccaa aaacatccac
     6181 ctgcaaaagt agcaagtttt atcattttca tcactcctta aataaattat actttattta
     6241 ttccctaaga agtaacttaa taatctgcat tttattattt ttacctactc aatgaaatca
     6301 tgtgcaaaaa tacaattgca aattgaatct aatttctata aaagctcttt ttctttatcc
     6361 atccaaaagc atcttttata tcacaatttt ctcctaaaga cattagatat tctaatgcat
     6421 cacaatcatc catagctaca tctttttcca tcttaggatt taatctatag taattatctc
     6481 caagaagttt ttgagaaaat aactgactca tttgagaatt tccttctaag gtaatagata
     6541 taattggtaa atcgggttct ttattaatta tccagtcaat agctccccat tcagtagtat
     6601 ctgcctttat actattatat acatatccag tccctattga aaaaagtctt gtattcttaa
     6661 gtttaaatcc actgtcaatg gcatgaatta tacacgctaa acttgggtca gttgctatta
     6721 ttcctccatc aacatgacga ttatatgttg gaaaaaatac tggagcagca ctacttgcca
     6781 ttgctacatc tacaactttg ctagtctttg taaaagaatt tggcatattg ttataaaaca
     6841 ctggtttcca tgcattttcc tcatttccta tataaaaact tggtatcatt actaatttat
     6901 ttaaatcttt taattctagt tcctctggaa atatacttaa taaaacttct tttaagtttt
     6961 tgttttcata ctttggtctg agtatttctg cataactttt attaaaaata tacttagaat
     7021 tttcaattga gtaaagttct tttatctctt tagggcttac accatacgca agtcctagtg
     7081 caattagact ccctgtagat gtccctccaa acatatttat attgtttaat agatttggat
     7141 actcttttac tatcctctca agtatggata tactcaatgc cccttttaat ccaccaccat
     7201 caaatgccaa tatattaaaa ttagaacttt tcatatacat atacccccac ataaaacacc
     7261 ttatatt
//
LOCUS       DAELSY010000175         7194 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.175, whole genome shotgun sequence.
ACCESSION   DAELSY010000175 DAELSY010000000
VERSION     DAELSY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7194
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.175"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(27..1475)
                     /gene="ddlR"
                     /locus_tag="KPG09_003356"
     CDS             complement(27..1475)
                     /gene="ddlR"
                     /locus_tag="KPG09_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBG0967920.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            complement(1499..2398)
                     /locus_tag="KPG09_003357"
     CDS             complement(1499..2398)
                     /locus_tag="KPG09_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBG0967921.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            2656..3912
                     /locus_tag="KPG09_003358"
     CDS             2656..3912
                     /locus_tag="KPG09_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBG0967922.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            3982..4761
                     /locus_tag="KPG09_003359"
     CDS             3982..4761
                     /locus_tag="KPG09_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0967923.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(4865..5449)
                     /locus_tag="KPG09_003360"
     CDS             complement(4865..5449)
                     /locus_tag="KPG09_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBG0967924.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(5649..7079)
                     /locus_tag="KPG09_003361"
     CDS             complement(5649..7079)
                     /locus_tag="KPG09_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0967925.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNADLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
BASE COUNT         2522 a          947 c         1011 g         2714 t
ORIGIN      
        1 aactaggcta acaatattta gttaacctag aacatttcat ttatagtttc tccaattatt
       61 tttattccaa tttcaatgtc ttcaaaagac aacctagaaa atccgagtct taaagtatca
      121 aaaccagagt tatctgtaaa aaataaatct ccaggcataa atataacatt tttttcataa
      181 catttttcta ataaaactct agtatctata ttttttagtt ttataaatat atgcaaacct
      241 ccatcaccca agatatattc tgtttttata tattttttta tacatttata tgcaaagtta
      301 aatttgtccc catagaattt acgtattttt ttaatgtatt tttcaaaacc tccattactt
      361 aaataatcaa ataaaattcc ttggtctaaa aaggatacat gtatgtttct gcaccttttt
      421 acactttcta gtttattgat tactttttta tctgcaaata tccatccaat tctcattcca
      481 ggaaataaaa tttttgaaaa actaccaata tatacgaccc cattattcat attatctagg
      541 gaacagatag gaaatatatg agaactattg tagagtaatt cttcattaaa tccatcttct
      601 ataattgcta tattgtattt tttcataaga ttatagaatt tgtacctatt ttcaggagtc
      661 attacaattc ctgtaggatt gtgataagag ggtgtaatgt atgcaaactt tattttattt
      721 ttatacttaa ctaaattgtc ttctagcata ttgaagtcca aaccatcttc atttatatct
      781 acacctaaga tattgaggtt atgagatttc ataattttta tagcagtatt atgagtagga
      841 ttttcacata ttatgtagtc tttttcttgg gtaaaagatg acataataat atcaaagcct
      901 tctgtgaatc catttgttat tagtatatct ttattactta tatcagcacc cttgtttgtc
      961 atatattcta gtaaatagtc aataaggggc ttataacctt gtgcatatcc atagttgagt
     1021 aatttatgtc cttctagtga aattctattt aaaaaggatt tttttaattc ttccatgtcg
     1081 aataaatctc catctggaga aatactttta aaggaaatca ggtcagagct ccaaggaatt
     1141 tcacttttta taatgtccaa ttcattggct tttttagagt atgtattctc taaacaatcc
     1201 cagtttaatg accatgtagt atttgaagac ttattctttg atttaacaaa tgtacctttt
     1261 cctgaaatag agtagattaa tccttctgat tttaattctt cataagttaa aactacagaa
     1321 tttcggctaa cttgtaaaag ctgactaagc tcccttgtag aagggagctt actattgtct
     1381 ggaattagtc cttttgatat catatcactt atataatttt taagttgtat ataaattggt
     1441 tcattatcat ttagttttag atttgaaaat atcaaattac cacctcaaat tatttcatct
     1501 aatttaaaga gtattctata agtttatcta atagttcact gtacttaata cctctagcag
     1561 cagcacttct aggaattaaa ctagtttgag tcattccagg aagagtattt agttcaagaa
     1621 catatggaat accttcactt ataatcatat caactcttac atatgctttg caattaaaaa
     1681 ttttccaaca agtctcagaa atttcgttta ctcttttttg aagttcttca tctaagtaaa
     1741 caacttcttc ctttgcacct ttttcaactg agtatttagc ttcataatca aagaatcctg
     1801 aatcagattt tatagatatt gtagggaaca cttctccatt taatatgaat gaagtatatt
     1861 ctccacctgg tatatatttt tcaatcatta caaattcgtc aacttccaga ccttttctaa
     1921 ctgcttcttc aacttcatcc ttagaatgaa taaagaatgt ggctacactt gaacctcctg
     1981 agtttggttt tataaatact ggataaccta tattatctat ttcatcatag tcgatttcat
     2041 caactgattt tactaaaacc catggagcag taggtaggtt tgaatctcta agcatttttt
     2101 tagttatatt tttatccata cacatcccac tgcaaagagg tccacatcca gagtatggta
     2161 tatccatagt ctcaagtatt gattgaatac atccatcttc accaaatttt ccatgtaaag
     2221 caagtattgc aaaatctata tcttctggta tctttgtaaa tatatctttt ttatcatcta
     2281 ttattacttt tacaacttca tatttatttt tatcaaggtt attatatatc tcttttcctg
     2341 aatttaaaga aacttctctc tctgatgata ttccacccat tataactgct attttcatat
     2401 tatacactcc tattttaatt tttattattt taaagtaaac tttttattta catacttaat
     2461 tatatgaatt aacagttgag gatgaaagta ccactatata cgaagtttat attatgtgtg
     2521 gttttagaat aataatctag tttaaagata tgtttaaatg attttttaac tttatttatg
     2581 ttattatttt tgctaaaatt attgttatga acttaagact agtcttaatg gctataaatc
     2641 aaggagtgat aaattttgaa agctgaaata ataagtgtag gaacagaaat attattggga
     2701 gatatagtaa atacaaattc tcaattttta gctaaagaac ttgcatcatt gggaatagaa
     2761 gtataccatc agagtactgt tggagataac aaacaaagac ttttagaatg ttttgatgaa
     2821 agcttaaaga gaagtgattt tgtaattact acaggtggtc ttgggccaac tggtgatgat
     2881 atgacaaaag aaactgctgc agaatacttt ggtcagaaat tagaactaca taaaccttca
     2941 ctagaggttt tggaatcttt ctttgttaaa acaggaaaaa aaatggccga aaataatatg
     3001 aaacaagtgt actttccaaa agatgctata gtactaaaaa ataataatgg tacagctcca
     3061 ggggcaattt taaaaaaaga tggtaaattt ataatagtat tgcctggacc tccaagagaa
     3121 atgaaagcta tgtttaatga gagtgtcaaa ccttacttac aacaattcac aaatgaaatg
     3181 ctagtatcta agactttaag attatatgga ataggtgagt caaatctaga attagaaatt
     3241 ctcgatataa ttaatgagca gactaatcca acagttgctt tatatgcaaa agaattagaa
     3301 gttacaataa gaataactgc aaaggctgaa aatgaaagag aagcttttaa attaataaaa
     3361 ccagtagaag aaaaaataaa aagtagagtt ggtaaatatg tatatactga aggagatatt
     3421 tccatatctg aaggggagac tgctttagaa gatgctgttt ctaagttact tgtagaaaaa
     3481 aatctaacaa tagcagtggc tgaatcttgt actggaggtt tggtttcttc atctcttata
     3541 aactatccag gaatatcttc tgtatttctt gaaggatgtg taacatattc taatgattct
     3601 aaaatgaaaa gattaggagt aaaaagagaa actttggaag agtttggagc agtaagtgag
     3661 caaacagcta tagaaatggc tgagggcgtt gctaaaggac ttaaagctaa tataggtata
     3721 tccacaactg gagtagctgg acctggtgga ggaaccaaag agaagcctgt gggactagtt
     3781 tatactgcaa tatatataaa tggaaaaaca atagtaaaga aaaatatatt taatggggat
     3841 agaagaaaga taagattaag agctactaga gatttactta atgaattgag aatacaatta
     3901 gaaaagcttt aagttgttat gatattggat atatttgaag tgttactata atataaaatt
     3961 gtttttggag gcttttaatt tatgataaaa catatatttt gtgatttaga tggaactcta
     4021 tatgaaaatg gaaccataac aaaagaagat atagtagcaa ttgaagaaat agaaaaaaag
     4081 ggagtacagt ttaatgttgc tacaggtaga atttttaaac aagcacataa tattatcaaa
     4141 gatagtttag atatgaatgg atattatgta tgtgaaaatg gttcatttat atatgataaa
     4201 gattataatg taatttttaa aagaacgata gatgataatt tagtaaaaaa agtcattgat
     4261 agatttgaat caagtgatgc acaactttat tttaaatata agggtgatgt aattgtgggt
     4321 agtgatacca ctgcttttag acattattct agtgacttta tagttgaccc agattttgaa
     4381 aaaagaagta gttttgataa tcttatagga aatataggcg tatgttctga aaatttggaa
     4441 gagttatcca gaatagaact ttatctaaaa agtgagttta gtgaagtatt agaaatatat
     4501 ttttctggaa catatacttt aaatatagtt cctaaaagtg tgtcaaaaag aggaagtata
     4561 gagcatgtaa taaaaacgtt aaatgtaagt ccagatgaag ttgctactat tggggattct
     4621 ccaaatgata tttgcatgtt agaaggattt aaatatagtt ttgcaatgtc taaggcaagg
     4681 gaagatgtaa agcaaagtgc aaattatgta gtagatagtg taaaaagtgc catagatgtt
     4741 ataatggaga taaatagtta gtataaaaaa tgactgtcct agagaagtat aaaattatat
     4801 tctatagagc agtcatttta attatattca attataagtt tatataaatt taaaaattct
     4861 taagttaacc gcaatttgct atatgatatg attttattgt attagataaa atagagtcaa
     4921 gtatataatt tatgacacta ttacaagtat aagctgaaaa ttttaaatca ttcatataat
     4981 cagttttctt tttgtaatca ctataaagtt tagtaaaaaa cttctcaaaa ctactattta
     5041 tagtaccaca tttaatttta gataaatcaa catcttttgc aaatgctcca agttctcttt
     5101 cataagtaac atgtttattt aagctatgct taaattccca acgttcactg tgagctacac
     5161 agaaaaaatc acataaaaaa tgacatatga ctcctagttc ctgactaagt ctacttactg
     5221 aaaagtattt agaaagacta tctatattaa gactgcatag atagtttatt ttagaaacaa
     5281 tcatattaaa tgactcatct aaataatgtt tttttaattt atatttagaa aaagcatcag
     5341 gctttatatt gccatatata aaattttttt cgcttataaa gaatgtttta ttttcatcta
     5401 tattatcaag tattgatttt gccataataa aatgggtatt cattaacata tttacacctc
     5461 gtatagttta ttagacttat taaatatatt attatgatat gatatttatg ataaaagttt
     5521 aatggtaaat atatgaaact aagtcaacag tatttattta aataatttca accatattca
     5581 taataacagt aaatggccaa atttacaata tgtaaatagt gttaagtttt tatttatcaa
     5641 atttaaaatt aagtattagt atactttcta attaacctat tggctttaga ctgtgagatt
     5701 tcaaggtgtt tggctacttt ataagaacta ccaaattttt cataagcatc caaaattata
     5761 tttttttcat actctttaat taaggtgtca aaatttttat ttttataatt agtagatttt
     5821 gagtcagata taataacctt attaatttgg gatatatgtg ttttatttcc attattggta
     5881 atatgtatat tttttgtaag aatgtttggt aagtcatcta ttgttatatg attatctgta
     5941 caagtaagca agattttttg aattatattt tgtaattctc ttatattgcc tgggaatcta
     6001 taatttgcaa tagcatccat agcttcttta gaaatagttt tatttaggtt gaagtcactt
     6061 gagtacctat taaaataata ttttataatt tccactagat tatcccttct ttctctaaga
     6121 ggaggcaatt ttatttctat aacatttaat ctatagtata aatcttctct aaactttttt
     6181 tcttttactt ttgaaactaa atcagcattt gtagcagata taattcttat atcaaccttt
     6241 ttatttttta agctcccaac tggagtaaaa gttctatcct gtattaattg caaaaatttt
     6301 gcctgtagat tttgtggtat atctcctatt tcatctaaaa aaagagttcc tccattagct
     6361 aattctacga gcccaacttt acctttagta cttgcacctg taaaagcacc tgaagtatat
     6421 ccaaaaagtt cagattcaag aagttgtgga gatatagttg cacaatttat tgttataaat
     6481 gggccattag atctattgct gttgtggtgt atatatctag caagtgagct ttttcctgtt
     6541 ccagattcac ccaataaaag tacacttaca tccgttttag aaacatgatt tactgtgttt
     6601 atgattggtc tcatcactat tccgtccata attacagtat cttcaattct aagtgcagtt
     6661 ctgtatagtg tttctacctc agatttatat ttttttactt gcttttttgt gtcttcaagt
     6721 ttatttttta tattattaag ttcagttata tctctacaat tttcaacagt aaattctaag
     6781 tttccatttt catcaaatat aggggtagca gttatgataa gctttttccc tgtttgagta
     6841 tcttgttcca gtgaaaattt agtctttgtt tttaatacaa ttggtatggg gctttttgtt
     6901 gaatatccag agtcttctaa aaaagaaaca ttttttccta aaatatcttc tttttttaga
     6961 ccataatttt cttcaaatgc tttattacaa tatattatta ttcctttatc atcagaaaca
     7021 catatttcat catgggaagc ttctaatatt ttttgataaa attccaaatc taaattcatt
     7081 tttatatttt cctcctaaat ttttaatgat atattaaaat aggtttaatt gattaatata
     7141 cttaaaattt gatatttata taagtttcag tctattttaa ctttaaataa gtta
//
LOCUS       DAELSY010000176         7150 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.176, whole genome shotgun sequence.
ACCESSION   DAELSY010000176 DAELSY010000000
VERSION     DAELSY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7150
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.176"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..665
                     /locus_tag="KPG09_003362"
     CDS             <1..665
                     /locus_tag="KPG09_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two pore domain potassium channel family
                     protein"
                     /protein_id="HBG0967926.1"
                     /translation="RIFEKVKFKGPDLVVNKVENFPRMDGIIFSMCTKFVLKNVEYGK
                     SEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMKRSNYPTFSDYLVEKFF
                     DQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNSTLVALDINNIGDYSLS
                     EIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFFGVTIGAIWASVIIKRM
                     IR"
     gene            769..1953
                     /locus_tag="KPG09_003363"
     CDS             769..1953
                     /locus_tag="KPG09_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG0967927.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADGKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNEKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGVIKYYTSSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            2160..2873
                     /gene="rpsB"
                     /locus_tag="KPG09_003364"
     CDS             2160..2873
                     /gene="rpsB"
                     /locus_tag="KPG09_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBG0967928.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            2952..3863
                     /locus_tag="KPG09_003365"
     CDS             2952..3863
                     /locus_tag="KPG09_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBG0967929.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            3948..4652
                     /locus_tag="KPG09_003366"
     CDS             3948..4652
                     /locus_tag="KPG09_003366"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBG0967930.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            4678..5235
                     /gene="frr"
                     /locus_tag="KPG09_003367"
     CDS             4678..5235
                     /gene="frr"
                     /locus_tag="KPG09_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBG0967931.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            5238..5441
                     /locus_tag="KPG09_003368"
     CDS             5238..5441
                     /locus_tag="KPG09_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967932.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            5470..6204
                     /locus_tag="KPG09_003369"
     CDS             5470..6204
                     /locus_tag="KPG09_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBG0967933.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6217..6996
                     /locus_tag="KPG09_003370"
     CDS             6217..6996
                     /locus_tag="KPG09_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBG0967934.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         2940 a          752 c         1388 g         2070 t
ORIGIN      
        1 accgtatatt tgaaaaagtg aaatttaaag gacctgattt ggtagttaat aaagtagaaa
       61 attttccacg aatggatgga ataattttta gcatgtgtac aaaatttgtg ttaaagaatg
      121 ttgaatatgg aaaaagtgag tatgagcatg gcaagataaa ttatagaata gctagaaatc
      181 aagcaacaaa aattggagaa tatgagatga taggatttta ctattataag gaaagaattt
      241 atagtagtaa aataatgaaa cgttctaatt atcctacttt cagtgattat ttagttgaaa
      301 aattttttga ccaaatagca cgttacacaa caggttatgg agaaaagcca tggaatatat
      361 tattagtgat tatagcaata ataagtgttt ttgctttatt atacttattt gtaggcatag
      421 aaagttcaaa tagcacactt gtagctttag atataaacaa tataggtgat tactcactaa
      481 gtgagatttt caaaatgtat atggatttat ggtattttag catggctaca tttagtacag
      541 taggatatgg agatatggtt gctacaagtt tgattgggaa agcattggca ggtatagaag
      601 tattttttgg agttacaata ggagcaattt gggcatctgt gattattaaa agaatgataa
      661 ggtaatattt gtgtaaatat ggataggtat taattactag atataaattg gaccaaatag
      721 ggaaaattta aatataaata tacttaataa tccaagaagg tgaaaaattt gaaaaatgca
      781 ctaaaaaaaa ttatatcatt agccatgatt ctagttttta taataccaaa ttattcatat
      841 gcagatggta aatctgtaga caagtattca aaaacatcta tacttataga ccaagaaaca
      901 ggtagagttt tatatagtaa agaacctgat atgaaggtac cattagctag tttaagtaag
      961 atgatgactt ttttacttgc aatagaagct attgaaaata aagaagtaaa agaaactgat
     1021 aaggtaaaaa tagacaaatc tattgcatca gtaaaagggt caagttataa acttaaagat
     1081 ggtgaagaag tgccacttat agagcttatg agaggtctta tgattgtatc aggaaatgat
     1141 gcggcaattg ctatagcaaa acatatttgt aaaacgaaag aagcatttgt agatagaatg
     1201 aataaaaaag caaaagaaat aggaatgagt aatacacatt ttctaaatcc aaatggtctt
     1261 ccaatatacg atttaagcaa ccccaaaaaa ccagcaaaag aaaatatctc tactgcaaaa
     1321 gatatagcta ttcttggaaa atacatgttt gaccattatg aaaaacaagt aacagctata
     1381 actgatatgg agacatatac aaattcagat agaagttttg aaaaaagcaa tacaaatgca
     1441 ttattaagaa ttataccaga agtagatggt ataaaaactg gatatactgg caatgctgga
     1501 tattgcttat cattttctat ggttgtaaat aaaaatgaga aaaatgagaa aaaacgtaga
     1561 gtaatagggg ttgtattagg tactaaccat aaaaataaaa gaacctctgc ttcattagct
     1621 ctactgaaat atggaaaaga aaatttcaat atgaaaaaag taattgataa agatagcttt
     1681 attggtaaga aatatataaa aggaatgaaa gagttagagg ttacattaaa agcaaaagat
     1741 gaactttata caatattaaa agatgatgaa agtataaaat cagaagttaa gattaaaaat
     1801 atagagtacc ctgttaaaaa aggtgatact atgggtgtta taaaatatta tactagttca
     1861 ggtgacttac ttggaagtgt agatatagta agtaataata gtatagataa cgtatcatta
     1921 aaaacaaaat taaaaatgaa atttgcaaat taaattcaaa aaatggtata aaatacacaa
     1981 aaaaatataa aaatagaaaa tatggaaaaa atattgttat ttcttcgcct ctgtgatata
     2041 ctactagagg tgaaaaaata aaataaatca cacacagcat tgggatcctt aaaaggtgcc
     2101 tattacatgg ttttttaggg agaagatcaa gctggaggta taaaaccagg aggtaagata
     2161 tgtcagtaat atcaatgaaa caattattag aagcaggtgt tcactttggt caccaaacaa
     2221 gaagatggaa ccctaaaatg gctaagtata tattcacaga gagaaatgga atatatataa
     2281 tcgacttaca aaaaacagtt aaaaaagttg aagaagctta caagttcact aaagaagtag
     2341 ctgaaacagg aaagccaatc ttattcgtag gaactaaaaa acaagctcaa gatgcgataa
     2401 aagatgaagc tgaaagatgt ggaatgtact ttgttaatga aagatggtta ggtggaatgt
     2461 taacaaacca caaaactata aaaacaagaa taaataaatt aagagaatta gaaaaaatgg
     2521 aagaagaagg cgtatttaat gttcttccta aaaaagaagt tataaaatta agagctgaaa
     2581 aagaaaaact agaaaaatac ttaggtggaa taaaagacat gcctgaatta ccaggtgcaa
     2641 tgttcgtagt tgacccaaga aaagaaaaca tagctattca agaagctcat agattaggta
     2701 taccagtagt tggtatagtt gatactaact gtgacccaga acaattagac tttgctatac
     2761 caggaaatga tgacgctata agagccgtaa aattaataac tggtgctatg gcaacagctg
     2821 taattgaagg tagacaaggt gctgaggaag aagtagctga agaccaagaa taataatttg
     2881 acaattaaat ggtaagggtc gcccttacca tttataatat ggaatagaat aagtaggagg
     2941 aaacttaaaa aatggctaat ataactgctc aaatggtaaa agagttaaga gaaagtacag
     3001 gcgcaggaat gatggactgc aagaaagctt tacaagaagc tgaaggaaac atggaaaaag
     3061 cagtagattt attaagagaa aaaggattat caaaagctgc taaaaaagct ggtagagttg
     3121 ctgctgaagg tctagttgca atagaaatga atgatgataa tacagttgct tctatggtag
     3181 aagttaactc agagacagat ttcgttgcta aaaacgaaga cttcaaagtg ttcgttaaag
     3241 atgctgcttg tatggcatta gctactgata aagaagatat agcttcttta ttaggtgaaa
     3301 ctcataaaga gggaataact ttacaagaag ttttaaataa tagagttgca aaaataggtg
     3361 agaaattaga ctttagaaga tttgctaaag ttgtaactaa tggacaagtt gctggataca
     3421 ttcacggtgg tggaaaaata ggtgttcttg ttgaaatgga aacagaagct agagatgcta
     3481 aagttttaga attaggtaaa gatgtagcta tgcaagttgc tgctatgaat cctaagtatg
     3541 tttcaagaga tgaagtagat gctgaataca tagcacatga aacagaagta ttaactcaac
     3601 aagctttaaa tgaaggaaaa ccagctaata tagttgaaaa aatggttaaa ggaagattag
     3661 aaaaagaatt aaaagaggtt tgtttattag agcaaacttt cgttaaaaat ccagatataa
     3721 ctgttaaaca attagttgct gatgttgcta aggcagtagg ttctgatata aaagttgtta
     3781 aagttgtgag attcgaagtt ggtgaaggta tacaaaagag agaagaaaac tttgcagaag
     3841 aagttgctaa gcaacttaag taataaccaa agagagaaca cgcaggtgtt ctctttttta
     3901 aaaaatagaa aaaattggat atgaaggatt gaaataggag gtttctgatg gataaaccaa
     3961 tgtacaaaag ggtattgtta aaattaagtg gagaagcatt agcaggagaa agaggttttg
     4021 gtataaataa tgatgtagtt aatgatattg ctattgctat aaaaaaaata caagaaatag
     4081 gtgttgaagt agctgtagta gtaggtggag gaaacttctg gagaggcaga accagtgaag
     4141 gaatggatag aactactgct gactatatag gaatgttagc tacagttatg aatgcaatgg
     4201 cgctacagga tgcactagaa aatatagatg ttgctacaag agttcaaact gctatagaca
     4261 tgagacaaat tgcagaacca tatataagaa gaagagcagt tagacactta gaaaaggaaa
     4321 gagttgttat atttggtgca ggaacaggaa acccttactt tacaacagat acaactgctg
     4381 ctttacgtgc tgctgagatg gaagcagaag ttatattgct agctaaaaat gttgatgcag
     4441 tatatgacaa ggatccaaaa gtacatgcag atgctaagaa atttacagaa ttaagttaca
     4501 tggaagttat acagaaagaa ttaaaagtaa tggattctac agctacatct ttatgtatgg
     4561 ataataaaat tccaataaag gtatttgagc ttacaacaga gaatataata agagcagtaa
     4621 aaggcgaaaa tataggaact actgtaaaat aatgaagaat gaaggaggag taaaattatg
     4681 aaactggaaa tacataagca attagaagag aaaatgaatg ggacaattga tgctttaaag
     4741 tttgaatttg gaactataag agcaggaaga gctaatgcac aaatgctaga caaaataaga
     4801 gttgattatt atggaactcc aactccaata aatcaaatag gtgctatatc tgtaccagaa
     4861 ccaagaatat taatgatatc tccttgggat aaatctgcta tgcatgaaat agaaaaagcg
     4921 atagctaact cagacttagg attaaatcca tcaaacgatg gtgaggttat aagactttct
     4981 gtacctgctt taacagaaga aagaagaaaa gaattagcta aaaaagctag taaagctgca
     5041 gaagaattta aagttagaat aagaaatgaa agaagagacg ccaatgagaa aattaaaaag
     5101 atggaaaaag gcggagaatt aactgaagat gaattaaaaa aagcacaaga tgaagttcaa
     5161 aaaatgactg ataaatttat aaaagaaata gatacattat tatctaaaaa agaaaaggac
     5221 ataatggagg tataatattg aaaaaatatt ataacctttt aggtcttcat gtagatgagg
     5281 taaaacaata ttttgatgag caaaatataa attataccgt aaaatctatt gaaggtaaaa
     5341 aggataaaga aaaacttgta gttcctagag ttataaaaat atctgagata ggtgatagtg
     5401 tagaactgat aatcacatac ttttctgact ccttagttta aggagtcaga acaatattgg
     5461 aggcaaaata tgaataacaa cattatatat gatatagact taaataacat acctactcat
     5521 attgctatta taatggatgg aaatggaaga tgggcaaaag ctagatttct tccaagaact
     5581 gcaggacata aggctggagt agaaactata agagatatag taaaagaatg ctcaaaatta
     5641 ggtgttaagc acttaacatt atatgcattt tcaacagaaa actggaaaag accgaaactt
     5701 gaagttgaca cacttatgaa cttattatct acatacttaa gaaatgaaat agctgaactt
     5761 caccaaaaca atgtaaaagt aactgctata ggagatataa gtgctttacc aaaaacttgt
     5821 ataagagaat taaactcagc taaggaactg acaaaaaaca atacaggagt taacttaaat
     5881 ttagcattaa actacggaag tagagcagat ataaaaaatg ctcttataga tatagtaaaa
     5941 aattgtgaaa gtggtaaaat agatataaac aatattgatg aagatattat aaaaaactat
     6001 ttaagcacaa agtctatacc agatccagac ttggtaataa gaacaagtgg tgaacaaaga
     6061 ttgagtaact ttttactttg ggaagttgct tattctgagt tttactttac tgatatacat
     6121 tggcctgatt ttaaaaaaga agaactacag aaggctatat acgtatatca aagaagggat
     6181 agaagatttg gaggacttaa gtaaggagat tattttatgc ttacaagaat tattgcttca
     6241 ctagctctgg ttccattatt tctatttgtt gtatatggag gtattcctct atatatagct
     6301 gagacagcaa tagtttatat tgcattgcat gagttttata aggcatttaa aataaaagat
     6361 gtacatccaa tattcattat aggatattta ttttctattt atttagctgt aaaaaatatt
     6421 tttaatttac cattagaata tacttatgca gtaattttta ttttattttt agcaagtata
     6481 atttatatgt tgatgggcaa aaacaatgtt atagatgttt cgattacgtt tttaggagtt
     6541 ttttatatag gagtattttt agattttata attataacta taaatggatt tgaaaaaggt
     6601 agtatatatg tatggcttat atttgtgata tcttttatga cagatatatt tgcatatttt
     6661 agtggttatt tacttggaaa acataagcta attcctaaag ttagtcctaa aaagactata
     6721 gaaggagcta ttggaggaat aataggaagt acactttgtt gtattttatt tggatattta
     6781 tttggcattg atttgcttca attagcgata ataggaagta taggaagtgt aatagctcaa
     6841 ttaggagatt tatttgcatc ttctataaag agatatgtag ggataaagga ttatggaaag
     6901 ataatacctg gtcatggggg tatattagat agatttgata gtgttatatt agttgcacct
     6961 tttgtatata gtgcgattaa attttttatt agataaagat tggttggatt taaaagagca
     7021 atctataatt gctagtaatg aaaatagcta tgtaactttt aaatgcagag atattctgca
     7081 gttattagtt acatggctat tttcattgtt caaaaattta gaagaccttg gggaatttta
     7141 taagaataag
//
LOCUS       DAELSY010000177         7121 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.177, whole genome shotgun sequence.
ACCESSION   DAELSY010000177 DAELSY010000000
VERSION     DAELSY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.177"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            229..2274
                     /locus_tag="KPG09_003371"
     CDS             229..2274
                     /locus_tag="KPG09_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0967935.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNILIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILNSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            2535..3734
                     /locus_tag="KPG09_003372"
     CDS             2535..3734
                     /locus_tag="KPG09_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBG0967936.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDICRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARNLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            3786..4481
                     /locus_tag="KPG09_003373"
     CDS             3786..4481
                     /locus_tag="KPG09_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG0967937.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            4494..5171
                     /locus_tag="KPG09_003374"
     CDS             4494..5171
                     /locus_tag="KPG09_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967938.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            5282..6883
                     /locus_tag="KPG09_003375"
     CDS             5282..6883
                     /locus_tag="KPG09_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0967939.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDDLRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDNKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGIIKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
BASE COUNT         2863 a          694 c         1322 g         2242 t
ORIGIN      
        1 taatatatta acgaatttat attaaatttt tgtttatatt ccaataatgt caaactaacc
       61 gttttagaaa ttaaattata tgtgaaaaaa gatagcttac attattttat tatggaaaat
      121 aatagtaaat ggtacaaaat ttgattttta agatttaaaa cttttttaaa aagtgctaca
      181 atagaccaaa ttattacttt ctatactcgt taaacaggag gtgggactat gaaaaaacat
      241 aatattttat ttgtatcaac tgatgacaaa ataaatatag atatatcaaa acaattggaa
      301 aatatatttg gagaattttg taatatagat aacttggtat atgtaaatag gataaatatt
      361 gaactgtcaa gctatgagct tgtagtatgt tcagacaatg atataaagga atatatacat
      421 aataatattg ataaaaatat acttattgta atagtacatc gtactataaa tattgaaaat
      481 ataaatcaga taataagtat agaaaatgat tctgatgtta tggttataga tgcttataaa
      541 gagtcagcag atgaaactgc caagataatt agaaaactag gattaattca tattaatcta
      601 ataccatatt atccaggttg tgataagtct aaatgtgaga ttggaattat tacgggaagc
      661 agaaacagca taccacaaaa cataaagcaa ataattgaca taggtgataa ggtaattgat
      721 ataaatacag ttatagaaat ctttacaaaa ctaaatatat ctatagataa attacatatt
      781 attaaggaaa aatacaatga agatacagta agtggatata ggtattatac aactatgaat
      841 aaaaccatga agagtttttt ggagattata gatgaaggta ttgcctctgt tgataaatta
      901 ggaaaattta tatattgcaa caaagtattt tctaatctag ttggaataga ccaaaatgaa
      961 attatatcta ataactttat ggacttattt agtgataagg ttgtcaaaaa aatatttttt
     1021 caagaagatg aagttaatga tgaagtagta aatttaaata acaaaaaact gataattaat
     1081 aaagtaaatg tttatgaaaa taatgaacga ataaagagta ttataagcat taaaaatata
     1141 agtgcaattc aagtgcttga ggacaagatt caaaataaac ttcagtcaaa aggttttgta
     1201 tcaaagtata catttgagag tgttgtaggt gagagtaaaa taataaaaga aaaaattaat
     1261 attgcaagaa aaatagcaac tacagacttt tctgtgttga tacttggaga aaatggaaca
     1321 ggaaaagaaa tatttgccca ggcaatacat aatgaatctc taagaaaaaa caagccattt
     1381 gttgcagtta atctatcttc tttgtcagat acacttattg aaagtgaact ttttggctat
     1441 gatgaaggaa gttttacagg agcaataaaa ggtggaaaaa tgggtatatt tgaaagagcg
     1501 catacaggaa caattttttt agatgagata ggtgatatat ctttagatgt acagcagaga
     1561 ttgctaagag tattacagga aaaagaagtt atgagaatgg gcggaagtaa aattatacca
     1621 atagatgtta gaattatagc tgcaacaaat aaagatttaa agaaaaaaat taaagaaggt
     1681 tcttttagag aagatttgta ttataggatt aatgtattac atatagagat acctagactt
     1741 agagaacgta aggaagatat acctcttata agtaagtatt ttttagatga aataaatagc
     1801 aacaaatgtt ttacagagga atcaatgcaa gctttaacat tgtatgaatg gccaggaaat
     1861 gtaagagagc ttaaaaattt aatttattat attgatacta tagttgagga agatagagta
     1921 gattacgagc atttgccaga acaatttaga tttgaaaaaa acaatacttt agttaatgaa
     1981 aatttcgatt caattatctt agatttcaaa caatctaatt tctttgagga aagtatatgt
     2041 atattgaaca gtatagaaac ttggaataat aaaaatatat tattaggaag aaataaactg
     2101 caagagattt taaaagaaaa agatatagtt ttaagtgtag accaaattag aaaaagaata
     2161 gataagttaa agagccatgg tttactttta tctggagtaa aaaaacaagg tagttttata
     2221 actgatgaag gaaagaactt tatatcatat gtaaaaatta agggagtgat ataggggact
     2281 aaaaaggcaa aacaccctaa aaaaatccct ataatggata gaaataaata aaattatctt
     2341 taagattaag ataattcaaa aatattgaat aaataatcta agaagattat ttttaataaa
     2401 acagtagtaa atatcaacct tactactgtt tttatttttt gcaagattta ttatacaatt
     2461 aaaatttata ttttggcata tcaattgcta cataggaaaa atataaaaaa ttgtagaact
     2521 tggaggatgt tgttatgaag tcagcttaca aaggatatga agcctatagt tatttagaaa
     2581 aaggaaaaga tatttcatct attgagatgt gtaaaggaga taaaagagtt aaagagtacc
     2641 ttattgaatt agatgatatt caagaagcta aggttaaaag tatagtggat gaaaaaatat
     2701 ttatatcatt acatgaacat ccagtattat ttccagaaca cctagaaaga gatatatttg
     2761 aatataatag ggaaggtaaa caaagatgcg cttatgaagc attatcaaga ggatattatg
     2821 atgctatatt tgataattta atggatggtg tatgtacgat aacatcaaat tcagggtgga
     2881 aatgggatga tatattaata gatttaggca tgagactatg tgatttagct catcaagatt
     2941 ttgtcataaa gtgtgaaaaa gtagaagata tatgcagagc taaaagtgaa ggtaaagtag
     3001 cattgatacc aacattagaa ggtgcagcac caatagaaaa tgaacttgat agaatagata
     3061 tactgtatgg atttggtata aggttaatgg gtataacata cagcgaatcc aatgcattag
     3121 gttcaggact aaaagaagag aaagatggag gtcttacaaa tttcggaaaa aaagctgttg
     3181 aaagaatgaa taaaattgga atggcaatag attgctctca cgttggagtt caaactacac
     3241 ttgatgtaat agaatatagc aaggacccaa taatattaag ccatgttggg gcaagaaatt
     3301 tatggaacac aaagagacta actccagatg aagtattaat agcatgtgct aaaaaaggag
     3361 gggttatagg tgtagaagca gcacctcata ctacaataac agagaagaat aaagaacatt
     3421 caatagagtc atttatggaa cattttgagt atattaaaaa tttagtagga atagaccatg
     3481 tagcttttgg accagataca gtatatggcg accatgtaaa attacactca gtactagctg
     3541 ctaaatttga tataaaaaaa ataacaggta caaaagaaca tcccaaagta gaatatgtaa
     3601 aaggtcttga gaacccaaca gaaacttcaa aaaacattgt cagatattta gtaaagcatg
     3661 gatatagtga tgaggaaata gagaaggtac taggtggaaa tatacttaga gtactaaaac
     3721 aggtttggaa atagatatat tagaaatatt aataaaattt gaggatttat agggaggttt
     3781 tataaatgga ttatttaaaa ttagctaatc atccactttt gtggatagcg agtacagtag
     3841 cagtatcaat agtagtggta caatccctta tatttgctat aaaatcttgc aaagttggta
     3901 aaacaatggg aataacagat aaacaaataa agtcagctgt taagagtagt gctatttcag
     3961 ctattgggcc ttcaatgact atatttgctg gaatggtatc gcttatagta actatgggtg
     4021 ggcctatagc atggatgaga ttatcattta taggttctgt aatttttgaa tcaatgtcag
     4081 ctggatttgg aacagatgca ttaggtataa ctttaggttc tcctgaaatg acaaaacttg
     4141 catttacaaa tgcagtttgg actatgatat taggttcttt aggatggata atattcacat
     4201 tactatttgg acataaacta gataagataa ccaatgttat atctagtggc aagaaatcat
     4261 ttataccaat aatatctcta ggagcaatgt tgggttcatt tgcatatcta aatgctgata
     4321 gggtgttgcg ttttgataat ggaacaattg catgtataag tggaatggct ataatggtaa
     4381 tattgtgtat gcttgaaaag aagaaaaata taaaatggtt aagagagtgg gggcttacaa
     4441 tatcaatgtt tagcggaatg ataatagctt cagtaatata aggggggagt tttatgagta
     4501 aagtaaaatc tatatacaat gaagaatatt taccttttat gataaggtat ggaagactaa
     4561 cactatcttt aggtattata gcagcattag ttcctggaat aattttgagc tttggatttg
     4621 gtattatgcc tccaatatca gcattattag caagtacaat ggcaattgtc agtatgagtg
     4681 ctccaaatta cataatagaa ccagtttctt attcaccaat cttaggtatc ccaggaactt
     4741 atatgagttt tctttctggt aatatatcta atatgaggct accatgttca atagcagctc
     4801 aaaaggcagc agaagttgaa tcaggtacag aagaaggctc gataatatct acaataggaa
     4861 tagctgtatc tattttagtg aatataagta tactaactat aggggtaatt ttaggaggaa
     4921 gcgtattatc taaaattcca gctgaagtag tagaaaaatt aaacttaatt ttaccagctc
     4981 tatttgggtc agtttttgga caagtatttt tacaagataa aaaattaggt ctagtagcaa
     5041 ttgtaatatc tgtattgact attatattat caaagcaagg tataatacca cagtctctag
     5101 tagttcttat atgtgtattt ggtactatat taattgcaag agctatgtat aaagataagc
     5161 ttagtgacta attacaatga aataatagtg aattttcaaa tatgaaatat aataatttga
     5221 ttttcaaata taaaaattac attaagatta tttaaaaatt ggtttttaag gagggaatat
     5281 tttgatatat gatttaatct taaaaaatgg atttataata gatggtacag gtaatcctgg
     5341 tttttatgga gatatagcaa ttaaagacaa cttaatagca aaaatagatt ctaagataaa
     5401 tagcaataca aataaagaga ttgattgttg tggaaaagtt ataacacctg gatttattga
     5461 ccctcatgtt catgaagaaa tagtagcaat actagatggt aaatttgaaa agtttctaaa
     5521 acaaggagtt acaacaacta taaatggtaa ttgtggacat tctataacac catattcttc
     5581 tgaaaatgta tatgaatata tgtataaaaa tggtctatta cttgaagaag aaaaaaagta
     5641 tcttatagat aaaaataaat gttggaataa ttttacagaa tattgtgacc ttatatctaa
     5701 aagtggtatt tctgtcaata tgggattctt gttggggcat gggactataa gatggagtgt
     5761 tatgggaggt tctaaggata gacctcctac agaaaaggaa aaaaatgaaa ttacagatat
     5821 aataaatgat ggaatgaaaa gtggtgcttt tggaatatct actggattgg catatatacc
     5881 aagtaagtat gctgatattg atgaacttgt tgatattgca aggcagatta aagagcatga
     5941 tggtatatat acatctcata ttagagatta cataggaaga tataatgcag taaaagaagc
     6001 catagaggtt gggcaaaaaa gtggtgctag ggttcaagta tctcacttaa gtccagttga
     6061 aatagaagca tttgatgaga tattaaaggc taggtataat ggtgttgaaa taatggttga
     6121 cacagtacca agaagttctg ggcattgtat gaagaaaaaa agagtaattc aatttattat
     6181 ggcaatatca tctagtctat ttgaattagg tatagatggg gtaatggatg atcttagaaa
     6241 tgaagaggga agaacactta tattaaaaga agcatttata cttggagata gggggagtat
     6301 aatacttcta aacactaaag atattaatat ggaaaagaaa tcaatacgtg aaatagctac
     6361 acaaaaagga atagatgagg acaagttatt actcgattta cttttagatg gagacgaaga
     6421 acttatcttt tgtttaggag gaatgtatag agctgatttc ccagataaac tacatgataa
     6481 taaaataatt gataatccat ttgttatggt tggttcagat tgtttgttta gtgtgggtgg
     6541 ggatatgtca tggtttgaac tacaaagaag aggagctttt ccaattttct tcaatatgta
     6601 cagaaagtct ggtgttagat tggaagagat tgtaagaaga gttacttcat taccagctag
     6661 gcaatttaaa ataaaaaata gaggtattat taaagaaggg ttgatagctg atatagcagt
     6721 tattgacatg gataactata gttattctag gtcagaagat attgattttt caaagccaca
     6781 attactagcc aatggtgttg aatatgtaat tgtaaatgga aaaattgcgt tagaaaatgg
     6841 agagattact gagaataaat gtggtgaagt attaaaaaga taaggtttta aaaataatag
     6901 gatttagaaa gtaaataaat atctaagtaa gtaaagacga agtttgggat ttatttaaat
     6961 aaaaatcatg cttcgttttt atttatagta ttaatattta ttttaataat cagataatat
     7021 ttatacaaaa aatatttata ctttatagtg aaaaaattaa tatttttaat cattaaatta
     7081 tgtaaaaaat tggataaata gataactaaa tcatttgaga a
//
LOCUS       DAELSY010000178         7087 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.178, whole genome shotgun sequence.
ACCESSION   DAELSY010000178 DAELSY010000000
VERSION     DAELSY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.178"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            519..5030
                     /locus_tag="KPG09_003376"
     CDS             519..5030
                     /locus_tag="KPG09_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBG0967940.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTNPP
                     IGSTNLDGTTPNTLLITVAGSDIINQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGIGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADVEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIINPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWN
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVINLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NTSTSEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPAS
                     ILSPEYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNLNNNTSTSEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVINAGPSTAQNVTL
                     TDNIPASILSPEYSIDNGVTFQPWNGSLSIGTLGAGEIRNIIIRGIVSQTAIGTIINT
                     ATVSSTTPDPNLNNNTSTSEIDISSSADISVIKLANKTEACVEEQIDFVIVVSNAGPE
                     NAQNVTLIDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVILLRGIIKSTC
                     LDRLTNIAEVTSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCC
                     NCCKNDFWC"
     gene            5487..5834
                     /locus_tag="KPG09_003377"
     CDS             5487..5834
                     /locus_tag="KPG09_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG0967941.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(5984..7033)
                     /locus_tag="KPG09_003378"
     CDS             complement(5984..7033)
                     /locus_tag="KPG09_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0967942.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWVQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
BASE COUNT         2639 a         1060 c         1133 g         2255 t
ORIGIN      
        1 atttaaaaag caatataaaa gcttgttatt ttcgagaaaa atagaccctc taaactcaat
       61 tagataaaaa taaaatacaa aaaattatat ctctgaatat ctcaagtaat taagtttaaa
      121 ttttacgata ttttgtttga tgatttataa tataaaagca tattcttaat cttctcaaat
      181 taaaaagagt aaaaaataac aaaaaaatca ctgacatttc actgacaaat tgtaattttg
      241 ttattataaa tacattgaaa atacttgatt attgaaaaat ggcataaaat aatacctttt
      301 atgccattta ctattgtttg atagttttta acttatgaca taagatattt tttaacatat
      361 acaataaaat ataaaaaaat tgatattgca aataaaaaaa atacatatta gatgttatag
      421 aaaaaaagcg cacttatagg cttaaaatgt cacataaaag agtttcaaag aataatatat
      481 tatgaacaca aattttgtaa tcacaaagga gtgataatat ggcaacagta tcaggacaaa
      541 tagtttttga tagaaataga agtgctacca ttgattctgg ggactcagga atagccaata
      601 taccagttgt attacaagat atagcaactg gagttagact tgtggttctc acagatgcat
      661 caggaaatta tgtttttact aatgttccaa atggtaacta tagaattgta gaagcttttg
      721 gaactacagg aggagtacca acaccaggga attttgcaac agcgatagta ggtccagttc
      781 caattgcaga aatgccacct attagttctg tgacaaatcc tcctattggt tcaacaaact
      841 tagatggtac aacacctaac acactattaa taacagtagc aggttcagat ataataaatc
      901 aaaatatatt aaatgggcct gtgatttata cacctataga gaatatattg gattcttgcg
      961 tgtctgtatc taatataaac cttattacag atgcagataa cggaacattc ggtttttttc
     1021 caccaggtac accaagtaat acaggacctg aaatagaacc ttacccaaat gtgacgccag
     1081 attttacgta tgttgtacca gacccaacta agtttacacc tttagatgga gaatatacag
     1141 tacaaaatat tatgacaaat gcaatgagta atgttatagg agcttggtgg cgtattgctg
     1201 accatactac aggaaatgaa acaggaagaa tgatggtggt aaatggtttt aatcctggag
     1261 ctgtattttt taaatcaaca gtacaagtta cacctaacac taattattta tttagcactt
     1321 ggattttaaa cctttttaag gtaacaggat atccaccacc acagctaggt gtaagaatat
     1381 ttgaccaaaa taataatata ctatatcaag caacattagg agctcaaatt ccagttaata
     1441 caaacgcacc agaatggaaa gaagtaggta ctgtgattaa ttctcaaaat aataatcaac
     1501 ttacagtaga attttttagt gaaggagaaa gcgccattgg aaatgactat gctatagatg
     1561 atgtttcttt aagagaaata gctgttccaa cctttactcc aataaaaaca agtaacacag
     1621 aaactgctaa tgtaggtgat ataatcacat atacagttac attagataat acttgtgata
     1681 gccctttaac taatgtattt tttagggatt taattccaaa tggtttagag tttatacctg
     1741 gaactgtaac ggttgatgga gtatctgtac caggagtaga ccctaataca ggttttccac
     1801 ttccagatat tggaggagga ataggggttg aagtaacttt tgatgttgtg gttgaaagta
     1861 ttcctaaccc aaatcctact aataatatag ctactataga ttactcctat acacctgtag
     1921 aaggcggtat accaaatgat ttttcagtag attctaatcc agttcctgtt gaagtaatct
     1981 cagcagatgt tgaagttaca aaattatctg aacctactat agtgaatcca ggagaggaat
     2041 taatatacac tataaaagta gtaaataatg gaccattccc atctgagaat gtggttttaa
     2101 cagatgatgt accagcttct attataaatc cagagtattc attagatgga ggagtaactt
     2161 ttcaaccatg gactggaagt ttaaatatag ggacattaga agtaggagag acaagagtta
     2221 ttataattag gggtattgta aatccatcaa cagtaggaat tataacaaat acagctgtag
     2281 tatcttcaac tacagcagac ccaaacctta ataataatac atctacaata gagacagaag
     2341 tgaatttatt agcagatatt ttagtaatga aaacagcaga acctaattca gctgttccag
     2401 gtactttatt gagatatact attcaagtag aaaacttagg accagcaaat gctgaaaatg
     2461 taatattaaa tgatgatatt ccagcctcta ttataaatcc agaatattca ttggatggag
     2521 gagcaagttt tcaaccatgg aatggaagtc ttaatatagg aactttaaac tctggaattt
     2581 cattaacagt tttaattcaa ggaacagtta gtttaaattc ttcagaatat atagtaaata
     2641 cagcaacagt ttcttcaact acacctgacc cagatttaag caacaatata tctaccatta
     2701 ttacaccagt taacccacaa gcaggtattt ctattataaa agtagctgac gaggatgtag
     2761 ctgttccagg agaagaattt gtatatacaa ttgaaatttt taatgaagga ccatctaatg
     2821 ctacgaatgt agttttaaca gatgatatac cagatgttat tttaaatcca gaatattcat
     2881 tggatggagg agcaactttc caaccatgga atggaagtct taatataggt acagttgcac
     2941 cagggcaatt aataagaatt atcataaagg gattagtaag ttcaactgct acaggagata
     3001 ttactaatac agcagaagta agtgcagatg taccagaacc agttacggat tcatcaacag
     3061 tgacaactcc tatagttcca tcagcagata tagaagtaat aaaaactagc aatatggata
     3121 cagcagttcc aggagaaact tttagttata ctataacagt tataaattta ggaccatctg
     3181 ctgcacaaag tatcgttttg acagatgata taccagatgt tattttaaat ccagagtatt
     3241 cattggatgg aggagtaact ttccaaccat ggaatggaaa tcttagtata ggcactttgg
     3301 atgctggaga aataagaagt attataataa gaggaacagt gagccagaca gcagtaggta
     3361 caattataaa tacagcaaca ataagttcac ctacacctga cccaaatcca gataataata
     3421 cttcaacaga tgaaacagat ataagtccat tagcagatat ttctgtgata aaaggcaacg
     3481 aaccagttgc tataccaggg ggaagattta tttatggaat tgaaattgca aatgcaggac
     3541 catcttttgc agagaacgta actttaacag ataatatacc agcttctatt ttaaatccag
     3601 agtactcaat agataatgga gtgactttcc aaccatggaa tggaagcctt aatataggta
     3661 ctttagatgc tggagagata agaaatatca taataagggg aacagtaagt caaacagcaa
     3721 taggtacaat tataaataca gcaacagtaa gttcaactac acctgaccca aatctaaata
     3781 acaatacttc aacatcagaa gcagaggtaa gttcatcagc agatatttct gtagtaaaac
     3841 gaagcaatca gactgttgtt gtaccaggag atgtactaga ctatacaatt gaggtgagaa
     3901 atgcaggacc gtctactgct cagaacgtaa ctttaacaga taatatacca gcttctattt
     3961 taagtccaga atattcaata gataatggag tgacttttca accatggaat ggaagtctta
     4021 gtataggcac tttagatgct ggagagataa gaaatattat aataagggga atagtaagtc
     4081 aaacagcaat aggtacaatt ataaatacag caacagtaag ttcaactaca cctgacccaa
     4141 atctaaataa caatacttca acatcagaag cagaggtaag ttcatcagca gatatttctg
     4201 tagtaaaacg aagcaatcag actgttgtcg taccaggcga tgtactagac tatacaattg
     4261 aggtgataaa tgcaggaccg tctactgctc agaacgtaac tttaacagat aatataccag
     4321 cttctatttt aagtccagaa tattcaatag ataatggagt gacttttcaa ccatggaatg
     4381 gaagtcttag tataggcact ttaggtgctg gagagataag aaatatcata ataaggggaa
     4441 tagtaagtca aacagcaata ggtacaatta taaatacagc aacagtaagt tcaactacac
     4501 ctgacccaaa tctaaataac aatacttcaa catcagaaat agatataagt tcatcagcag
     4561 atatttctgt aataaaatta gcaaataaaa cagaagcatg tgtagaagag caaatagatt
     4621 ttgtaatagt agtatcaaat gcaggaccag aaaatgctca aaatgttact ttaatagaca
     4681 atgtttcaga taagcttaag aaagcagtat tttcacttga tagaggtgta agtttccaac
     4741 catggactgg aagtcttaat ataggtacac taccagctgg aactttaaga gttattctat
     4801 taagaggaat tataaaatca acatgtcttg atagattaac aaatattgca gaagttactt
     4861 caacaacacc agattcaaac cttaacaata atatatcaag agtgcaagta gaaataaaac
     4921 aatgttgttg taatgattgc tgttgtaaca actgtagtaa ttgttgttgc aatgattgtg
     4981 gttgtaaaga ttgttgctgt aattgttgta aaaatgactt ttggtgttga ttttataaaa
     5041 taattttgta taccttaaat taaactgaaa taaaaaataa attcacatat gagtaaaata
     5101 ttttataaat attaataaat tttctatctt tgatatggac cacattcgta atactagtta
     5161 ttaaatgaag tggtcctttt taattcatta aaattattta ttctaattaa attttaaatg
     5221 tattaaataa ttttaatttt ttataaaaaa tattcatata ttctttatat taaagtttag
     5281 atatctaaaa atacttttag aatttattat attatgttaa tttttttata tcttataatg
     5341 attatatttt tctaactata aaaatagtat ttacttatat ttaaaaataa aagaatttta
     5401 ttcctattgt gtaaagtgag aatactattt tcttatattg gtataataat agtaaatcta
     5461 taatatagaa aagaggtatt tctagtatgc atgaaaataa aaagaatcta ttagacggaa
     5521 gcttagctca atataatgat acagcagttc caaaaatacc agtatttgct ggtaacgata
     5581 taacttcaga agtttattat tttaaaccta atcaagttct taatgctcat agacacccta
     5641 atggtgaaca aatatttgtg ttcctaaaag gagaaggtaa aatgaaactt ggtgagcatg
     5701 aatgtgatgt aaaaaacggc gatactgtat ttgtaccaac aggagagtgg catgaaatta
     5761 ctaatggaag caatgaagaa atggtagccg ttcaaattac aaaaattaat gctggagcag
     5821 aatacagagg atagatatat aagtacagtt agtactttaa tatctatttt attatataaa
     5881 ctttaatctc aaatataaaa agagtctttt aaaaggctct ttttatattt ttgttaattt
     5941 tcattataaa aacaagaaaa tatctagcta aaactaatat attttattta atatttttta
     6001 tagcattgtt tgcaaactca gtatttacga tatctttata aggtgcttcc ttatctagtt
     6061 ctttagcttc tttcataaca gtcatcaatt tcttcaaacc ttcctccttt aatacaggat
     6121 tttgagacca tgcatctatt gatttatatc tatctacaac tgttgttaaa tcttctaaag
     6181 atatatcttc aaagaaaggt tgcatagcct tagctacttc tttgctagat gcttgttgaa
     6241 cccataattg acctttataa acagcatttg taaatctttg aattaaatct ttattgtttt
     6301 tcatgtatga tctcgtagta gagtatgctg taaaaggtat ttctccagaa tcttttccta
     6361 tagatgtaac tatatatcca ctaccttctt tttccattgc tgttgctgta ggttcaaatg
     6421 ctgttgtaaa gtcaccctct ccacctgcaa aagctccagc cattacacca aattgtacat
     6481 cagttcttac attaacattg cctgctttta gattttctcc aatagtaaga cctttttctt
     6541 ttagaacata ttcaaaagtc attagtggaa ctccaccttt tctaccacct aatatttctg
     6601 ttccttttaa atcattgtaa gagaaattag gcattttttt cctagcaact agaaaactac
     6661 catctctttt tgtcaattga gcaaagttca ctgcataatc gtttttgttt tgattatata
     6721 catatataga tgcttcagga cccattaaac ctacgtttgc ttcacctgaa atcaaagctg
     6781 ccatagtttt atctgcacct tgggtattta taaggtcaat ctttatacct tcatcttcaa
     6841 agaatccttt cgtaatagca gcatactgtg gagcataaaa aacagagtga gtaacttctg
     6901 ctatagttac tttatccaga tttttagacg aagaagattc atcttttttg tcattagagt
     6961 taaaatcaca tccagttaga catattgtac ttacaatgat agagcttgct attgcgacta
     7021 tttttttaaa cataataaat acctcccata agacatacga atagtatata gtattaaaaa
     7081 tttattg
//
LOCUS       DAELSY010000179         7071 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.179, whole genome shotgun sequence.
ACCESSION   DAELSY010000179 DAELSY010000000
VERSION     DAELSY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7071
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.179"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(258..920)
                     /locus_tag="KPG09_003379"
                     /pseudo
     CDS             complement(258..920)
                     /locus_tag="KPG09_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(1107..1616)
                     /locus_tag="KPG09_003380"
     CDS             complement(1107..1616)
                     /locus_tag="KPG09_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG0967943.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLE"
     gene            complement(1691..2104)
                     /locus_tag="KPG09_003381"
     CDS             complement(1691..2104)
                     /locus_tag="KPG09_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG0967944.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNSDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2541..3200
                     /locus_tag="KPG09_003382"
     CDS             2541..3200
                     /locus_tag="KPG09_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBG0967945.1"
                     /translation="MDEKILELLQEIQGNISNMQGSISGIEGTVNGMQGNINYMQGNI
                     NYMQGTISDMQGTISDMQGSINGIEGTISDMQGSINGIEGTISDMQSNINDIEGTISD
                     MQGSISSMDGTINSMQDSITDMQGEIKEVNSRLDNMEPDIKDLKTGQVETSSRLDTIQ
                     SDVTEIKSDVKINRNTLDTVIKHTSSLVEDTSDIKNDLNNIEIVTSKNWNDIAKLKSV
                     K"
     gene            complement(3807..3986)
                     /locus_tag="KPG09_003383"
     CDS             complement(3807..3986)
                     /locus_tag="KPG09_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0967946.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(4211..4744)
                     /locus_tag="KPG09_003384"
     CDS             complement(4211..4744)
                     /locus_tag="KPG09_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG0967947.1"
                     /translation="MQYISYYHSPIGNILLASDNVGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGEEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     HKVAQQKGISKMSAQAIGGAVGHNGISIIVPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            complement(5562..6134)
                     /locus_tag="KPG09_003385"
     CDS             complement(5562..6134)
                     /locus_tag="KPG09_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG0967948.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGSCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(6196..6459)
                     /locus_tag="KPG09_003386"
     CDS             complement(6196..6459)
                     /locus_tag="KPG09_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG0967949.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDLYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(6459..6680)
                     /locus_tag="KPG09_003387"
     CDS             complement(6459..6680)
                     /locus_tag="KPG09_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG0967950.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYSNPKNRVRAKKGGNMY"
BASE COUNT         2472 a         1197 c          856 g         2546 t
ORIGIN      
        1 ttacctgctg attgcccatt ttaaagtact taggatttaa ccttatacca tctaaccaat
       61 tttttctact ttcgtaactt tcacacttag gtttatttca tccttatgtt ttagtttggt
      121 tagctttagg ggtttccagc aattcaagta gtattggata ggttctatcc tatctctaca
      181 tataggtttc cctatatgct gactatttta gctactaact catgactaaa gtcacaagtg
      241 tgcgtagcta ttttttaatc aaactggaat acagactcca ttcctaggaa attggctgaa
      301 atatagtctg ctgtcaaaga tgctgtcatc acagaaatta aaacagatac tgaaaagttt
      361 ttatggactt cttcaagtga aaacataaca cctgccagtg gtgcatgaaa ggctgctgat
      421 aatccagcac ttgccccaca ggttaacagg aatttttcct ctgtcttccc tcggtcaaga
      481 gctctagaaa tacctttacc tgccatagca ccaagctgaa tggaaggacc ttcccttccc
      541 aaagcaagac cacccataag tgataagaat cctccaataa attttgtggg caacactcgc
      601 caccatactt gttttatttt ccctgtcatt tccccctcaa gctgagggat accacttcca
      661 gaaatcattg gctcccagtc caccaacttt gatacaatcc acgcaagtac tgcaagtact
      721 gcaaaccaag ctattatttt tattgtagaa ccttctacaa attttaaaat ttggctaagc
      781 caagtaccag cctgccctaa aagtatcctg tataaaagca caatcaaacc agccacagcc
      841 ccaaccataa gtccttcacc aatcaaaatt acctgaaagt gctctgcccg tttgataata
      901 tgagatgtat cctttttcat actgttcctc cttcatccaa taattctaca caatctgaac
      961 ctccaatgac taaagtcatg agtgtgtaca gatatttttt aatcaacaaa aatagatttt
     1021 tatttactaa atataagaaa ataattgttc aatttctcaa taaatgtaaa tttattcttt
     1081 taggtattta ttctccacag tcgtgcttat tccagcaaaa tatttattag ttcttgcttt
     1141 ctctgtgtat agattttacg attgtcctca aactgtaatg ctaatttttg tttatgacta
     1201 tcatagagtt ttgctttgtc tgtaaaagta tttagataat ctcgaaaatt gatataatta
     1261 ttccatgcga cactattcca ttcaacaaca tgaatatggt gagtacgagt atttttctca
     1321 aaatctccca tagcaaaaag taattgtttt ggtacatcct gacctcgaaa aacgattcca
     1381 cattgctcta acctttcgag gtataattta gcttcctcta aagaagcaac acctacaaca
     1441 atatcaataa taggctttgc gctaatactg ggaatagaag tgcttccaat gtgttgaata
     1501 tcaatggcag tcttatccca tatacttttc aataatataa tgacatcttt tgcactttta
     1561 tcccatatca tctgatgttc caataattta accataccac attttaaacc tatcactata
     1621 taccacctca atataatcca cctttatata tatattacaa ttttttctat cttgataaac
     1681 agtgatctag ctatttcact ttttcttgac actcgctaca ttccctatca tgaatagata
     1741 taattcctcc acatttagaa caagtatatc tctccttctg cttttgcata aacttctcta
     1801 aaccgtactg ctgaacaaat ttactatttt ccataaggct tgcttgatat ctcttgttat
     1861 aacttttttc aagatttttt atgagtttac aaggaaaatt gaaacattca aaacaataaa
     1921 acaacccttt ttgacagaca caatccttta ttttacactt acggcagtgt tctggctttc
     1981 ctgtatcgct gttcaaacaa ccagcacatg gctttttgtg ataacaatgc ttatagcaaa
     2041 ccatacaatt cattccacaa ggagcaaaca taatcgtatc aatactctct tttggcattt
     2101 tcataacatt ttactccttt caaaaatcca atttgtcgaa ttgttatata ttcatattgt
     2161 aacataaaaa atagctaaaa aattcatgca ctttcattgc caataaattt tcaaggaaat
     2221 atgaaagttt aaaactaaga ataaaaagaa ataaaaagga agctactaat caaaatattc
     2281 aaacacaagt aatcaattat gatattacca gcagttttaa atacaaatat aaccacatat
     2341 gcctcaaaaa cacttattat agtatcttag acacttagac aatacacaag attcattaat
     2401 aaaaaagttc tcaatataga tatatttaac catgttttga atagcagata attatatgat
     2461 taaaaattaa atatgacata gcgtttgtgg aatataacac ctaaattatg ttataataat
     2521 aaaacaggag tgtgatagtg atggatgaaa aaatattaga attattacag gaaatacaag
     2581 gtaatattag taatatgcaa ggtagtatca gtggtatcga aggtactgtt aatggcatgc
     2641 agggtaatat taactatatg cagggtaata ttaactatat gcaaggtact attagcgata
     2701 tgcaaggtac tattagcgat atgcaaggca gtatcaatgg tatcgaaggc actattagcg
     2761 atatgcaagg cagtatcaat ggtatcgaag gcactattag cgatatgcaa agcaatatca
     2821 atgatatcga aggtactatt agcgatatgc aaggcagtat cagtagtatg gatggcacta
     2881 ttaatagtat gcaagacagt attactgata tgcaaggtga aattaaagaa gtcaatagta
     2941 gattagacaa tatggaacct gatattaaag acttaaaaac tggacaagtt gaaactagta
     3001 gtagacttga tacaattcag agtgatgtta cagaaattaa gtcagatgta aaaattaaca
     3061 gaaatacatt agatacagtg attaaacata cctcttcatt agtagaagat acatcagata
     3121 ttaaaaatga tttaaacaat atagagatag tcacatctaa gaattggaat gatattgcta
     3181 aattaaaatc agttaaataa tataccatag taaaaaatac ctgaaaattt cacatacttc
     3241 tttaaagtta taccaaattt tatacagaaa tactgattat ccgacaacac actttactta
     3301 taaataattt attaaaaaat actcatttta tcctaaaatt aacacgacta aatgagtctt
     3361 gatataataa taaaaaataa gggagattga acactaaaaa gttgtgtatc tcccttatat
     3421 tctataatat agttatataa tttttcagcc taaaataaaa gtccatcttt gattaaaaca
     3481 tatcccctta tcacttataa aaggataaaa tcttgttttc atttttccat ttatatcttc
     3541 ttgcttaaga gtaataattt tttcttttga tactttaagt gtttggttaa atttaaccaa
     3601 tcgactaagt tttgttcaac atctattttt catattttcc aatacttaat ctatttttta
     3661 taaataattc ttttatttct aactcactta cattttttac ataaaacgaa gcatataaat
     3721 ttcaacctta tcttgtatgg ttcttaatac ttaatgagta gcttaagtat caatcattat
     3781 aaattttccc cttatattta acactcttac catttccaaa atatacaaaa tatattttga
     3841 acatttgtac aatgatgaaa tacaatacat gaaaatacaa tatcataact gttatctaaa
     3901 aatagaagtt cttcaacata acctttcaca aagaacatat tatctaagta ttttttgtct
     3961 gctccttgtt ttcctgtttg tagcatataa attactgcat ctaaacatat aactactcac
     4021 acgaaaaaag aaaataaact tccaaaatac aagctctaaa actacctcta aagttatata
     4081 atgtttacta ggaataaggt tgtataacat atagtttaaa gaatagtttt ttaaaattgc
     4141 aagttattgc tttcaaaatt agatgtttat atatcaaata aatgattaag aatttctaaa
     4201 tttaatggct ttataatgca ctccctttct ttgggataaa ataatgttgc acagatactt
     4261 tttctaaaga taacaatttt atttttctat caattccacc agcataccct gtcaaactcc
     4321 catttgttcc tacaacacga tgacatggaa caataataga gattccatta tgccctactg
     4381 caccaccgat tgcttgtgct gacattttag atattccttt ttgttgagca actttatgtg
     4441 caatttctcc ataagtgatt gttttaccat atggtatttg ttgtaaaatt ttccacactg
     4501 tagtctggaa aggagtacct accatatgga gtgatggtgt aaaattgggc tcttctcccg
     4561 aaaaataaat atctagccat ttctttgtct gctcgaaaat tggcaattct ttttcttgat
     4621 gttcttggtc aagattataa gcataatact tttgattctc aaaccaaaga ccagttaacc
     4681 caacattatc tgatgctaaa agaatatttc caattggact atggtaatag ctaatatatt
     4741 gcatacaatt cctccttata ttctgaaaac ttcactcaaa tcattagaca cttcttttct
     4801 atctgccatt atgtaaaaac atactaatat aatttccttc tgactacact ttcgttataa
     4861 tatctatcct catatcatat tagttttata tttacctgaa tctgttcatc ctttttttcc
     4921 tttccctttg atggatataa aattcataca tttaagcttc ctactgtatt aacttttata
     4981 gaagtgtata ttataatttt atcactaaat agacaaaaaa atatcgatat aacatcaaga
     5041 aatattttac tgagtttcgc ttattaaaag caactgaatt tctaaagggt acagatgagc
     5101 taataagtga tattgatata aaagttgttt ttcattaagt aatctatttt agtaaatgtt
     5161 ttaaagaaaa aacgggatat tctccaaaag tgtacagaaa tataaaaaaa gagatatgat
     5221 tttttatttc ttttgattat ccaaagagta aataaaaaat attgtaaaaa acttactaac
     5281 aacggcaatg ctttgaacta tggtaatact ttgaactttt gattggactt tttttgctaa
     5341 aataatatat ttaaatagca aagtaatctg tttaatcaat aaaaaataag agataaaaga
     5401 aatcaactaa aacctttacc tcttattttg atagtttatc agagctaata tttacactct
     5461 ttttattttt gatacttcat taaaaatgat gcttattctc ttttcatttt taatacttca
     5521 ttaaaattga tgcttactct ctattcactt ttaatactta attagaactg atgcttacac
     5581 tctgtaaact cataaacatc cattaatgct tcaccaaatc tttgatagtg aactacttcc
     5641 ctctcaccca aaaatctcaa tatatctttc aaatcagggt catcagttaa atttattaat
     5701 tgatagtacg tagccaatgc tttttgttca gcagccatat cctcatgtaa atcagttacc
     5761 gaatcgccaa agacattaat ataacttgta gtaaagttta ctccattaga atctactggt
     5821 tgaagaccat gtccaaaatt agcataatta ctaccaagtc cagcagcctt aagctcttcc
     5881 ggagtacaac tgcctattaa ttgatataac actgaagaaa ttatctcaac atgggccatt
     5941 tcctctgtac ctatatcagt taaaagtgcg cgagattttc cagttggcat agtatatctt
     6001 tgttgaagat atctagttgc agctgccaac tcaccattag gtcctcccaa ctgagtcatg
     6061 atatattttg ccattctagg gtctgcttgt cttatgttaa ctggatgttc cagtgttttt
     6121 tgataaatcc acatattatc ttactcctcc ttaatatata tttaataata attctaattt
     6181 taaaacttaa taattttatt tataaaatga tttgtcccat ggccaaggtt cagatatcca
     6241 tgaccaagga tattcacttg attcatatcc aaaattagtt aatgctccat atttagattc
     6301 ataatcacac acagcttgtg taaatttatc gcagtatcta ttgtataaat caagagcttt
     6361 cttatccctt ggattgttat ctaaatataa atttaattct atacaggcaa attgatattc
     6421 tgaaattctc tctaaaagtt ctcttctatt taatgacatt agtacatgtt accccctttt
     6481 ttagctctaa ctctattttt aggatttgaa tatagccaat cattatagtt atttgactct
     6541 attaaatcta attcaggaaa taaagtacct ctttttaatg ctgtttttgg attgtatgtg
     6601 tcatcataaa tttgacgatt gatgtatggt ctagcaaatt cacaacaata gtcttgttta
     6661 tcctctttta aatatcccat taattatcct ccttaatttt tagaatttta aaatagcatt
     6721 atttttaaaa attgtatact tgttctatat tatagtcagt agttttaata ggtctttaat
     6781 aagattaaat aattaaataa gtatcttttt ttgttcttat tttacctata aaaattttcc
     6841 taattaatct ttcataaata tctattttaa ataaataata tagagtttat acaatttttt
     6901 ataacttaat tactatttac tatatattat tcatttttaa ataaaatgtg attatttttt
     6961 attaaaaacg atataaatat acaatttaat actattttta acaaataaat ttatattaaa
     7021 atacattaat ttatatagtt ttatttattc taaaagtata attataaaaa t
//
LOCUS       DAELSY010000180         7053 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.180, whole genome shotgun sequence.
ACCESSION   DAELSY010000180 DAELSY010000000
VERSION     DAELSY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.180"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(15..839)
                     /locus_tag="KPG09_003388"
     CDS             complement(15..839)
                     /locus_tag="KPG09_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBG0967951.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     TLFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(850..2253)
                     /locus_tag="KPG09_003389"
     CDS             complement(850..2253)
                     /locus_tag="KPG09_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0967952.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIREYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(2257..2982)
                     /locus_tag="KPG09_003390"
     CDS             complement(2257..2982)
                     /locus_tag="KPG09_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0967953.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDVQNESKNLNKNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(3094..3867)
                     /locus_tag="KPG09_003391"
     CDS             complement(3094..3867)
                     /locus_tag="KPG09_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0967954.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFLGFLLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNEPIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVRYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(3873..4760)
                     /locus_tag="KPG09_003392"
     CDS             complement(3873..4760)
                     /locus_tag="KPG09_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967955.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDANYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVFDEP
                     TAGIDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSGDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(4774..5796)
                     /locus_tag="KPG09_003393"
     CDS             complement(4774..5796)
                     /locus_tag="KPG09_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967956.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETERYYID
                     KEKGCIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            6371..6547
                     /locus_tag="KPG09_003394"
     CDS             6371..6547
                     /locus_tag="KPG09_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967957.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         2464 a         1122 c          640 g         2827 t
ORIGIN      
        1 aatatcactt acaatcaaaa accatttatt aagtatattc ctaagatata caatatatat
       61 acatggattg gatagtataa ataaaataaa tacttcatac tcttcccttt ttctccattg
      121 taaagtaaca ttaaaggtag agaagcaatc atcatccatt ggtaattttg taaaatagaa
      181 ttttttaaac tataatctcc atttgacatt gttgtaaata taaatataca catcaaaaca
      241 taacaaatac tcaaactgaa ctttttacct ctacacaaat aaaaaataat cccaagtata
      301 atccaaattg gtccaccttc tactaaaaat ggtactggta ccaaaatcat aaataaagta
      361 ataactctgt tagtgacttt aaaaagtaaa gctactgtaa atattcctaa aattatagga
      421 atgattatca tcaataatcc tataataaaa tatttaattt ctttctcttt tctaaatttc
      481 tttatgaact ctattgcttg taaataaatc gctattaaaa ataatgttga aaaaatgtta
      541 tttataatta tagctccttc tggatgtgaa aatacattat taattatttg atttcctata
      601 gccataataa cagaccaaat atacaatcta cttatatatt tttttctgtt actagtgtga
      661 taaaatcctt caacagccat gaatataaac agtggtgctg atattctgcc tacccaatga
      721 aaccaaattg gtatttgcat tgtttgtggc ataaatgctg ctatatggtc aaatgtcata
      781 aatattaatg ctaaaatctt aatagtaaac ccacttatac cttttttaaa aaaatccata
      841 tcatatctcc taattttgtt ttatctctat aataattttt gttcctattc caatttcact
      901 acttacataa attttacctt tatgaatctc tattatttct ttagaaatag ccataccaag
      961 acctgaacct ttatgcatct cacctgtatt agtacctcta taatattttt taaaaatatg
     1021 tttcaaatca cttttgctta ttccaattcc attatctttt attataattt ctatattatc
     1081 tttttttaca atttctacac ttatcaaagt tccttcgcta ttatgaacta ttgaattaaa
     1141 tataagattg gttatagccc ttctgaataa gattgaatct acatatactt caattaaatc
     1201 ttcattactt tcaaattcaa tattcctatt tttatatcta ttatcattta aaatatcaat
     1261 tattatatct ctgactaaac tgactaaatt tatttttttc ttgtccagaa ctatagtatt
     1321 attttttaat cgagtagata aatttaaatc atcaactaaa tcttttatat acttagactt
     1381 attatatata atttctgtat actctcgtat ttcatcttct tcaaactcat aatctttgtc
     1441 attaattatc tctgcatacc cttgtataga tgctagaggt gtctttatat catgtgttat
     1501 atttgccagc cattcctctc tcaactcttc attttctttt ctctcaactt catttactct
     1561 taaagtatca gctaacatat ttatattttt aaaaacttct tcatatatac ctctgtcctt
     1621 aagatataaa ctataatttc cattagccat agtctcaata ctccatagta cgcttgaaat
     1681 tggttttgta agttttctac taaataaata tcctacacct aaagctacca cgctatcaat
     1741 tactagtgtt ataatgataa ctttatttaa agttttttta atgttatttt gagaataggt
     1801 taaaattatt cttgatatat ctctactagg tatacctact attatgttta gatgtttttt
     1861 gtttaaatat ttttctagta caaaattaac atactttgtt tctatatact tgtaattatt
     1921 caccatttga aagggtgtat atttttttgg agtatccttt ggcacattta ctccataaac
     1981 ttcctctcct aaatcattta aaacctgtat ccaggaattt gatttttcta atttttcagc
     2041 tccaatctta gatagtttaa attctccatc tttttcatat aaatcttttt caaaagaacg
     2101 tgcaaattct tcaggattat ttccagaatc tacaacttta aaaaagctat tagttgaaat
     2161 cacatacaat atagatataa tatttataat tactactgat atagctacaa acacaattgt
     2221 aaatataaaa tttctagtaa tcttccactt catattctaa tcttccttta ctgctaattt
     2281 atatccaaga ccttttattg tcagaatata ttcaggcttt gaaggattat cctctaattt
     2341 ttccctaatt tttcttatat gaaccattat agtattgtca aatccaacat aatcttctcc
     2401 ccacacttta tcccatagtt tttctttact tataatttga ttttgattat gtgcaaataa
     2461 agataggatt tttagttcct ttgcagacag tcctatagat tttccatctt ttataagttc
     2521 tgccctactt tcatttaata tatatgggcc aaatgatata attttttctt cattcttatt
     2581 tagattttta ctttcgtttt gaacatcact aaaataactt aatcttctca aatgcgcttt
     2641 aactctaaaa gccacttcct tagggctaaa aggttttgta acataatcat ctcctccaat
     2701 cgctagtcca agtaacttat ctaattcttc ggttttagct gataaaaata ttattggaga
     2761 attggaggtt tttcttattt ctttacaaat atcataacct tcgccatctg gtaacataat
     2821 atccaataca atcaaatcag gattaatact tttaaataat tctaatcctt cttttttagt
     2881 ttttgcagta tatatattat taaggccctc tttttttagg acagtttcta aaagcttcaa
     2941 tatttctact tcatcatcta ttattaaaac acgattttcc ataaacaacc tcctgtaata
     3001 atttacatat atattagcat atattttatc taaaattcat ttatcttatt tttaattaaa
     3061 ctaaaatata aataaaatta ctattatttt tagttaaaac cttttgcttg atgtaaaatt
     3121 ttcatatgtt attttagtta acactatcat taatataatt gagattacta tagaaaaaac
     3181 tatcaaaata cttggtacat tatacctaac catttgttgg ctcatcataa tccaatttgg
     3241 agtcactgac ataaatttta tagctttatc tggtataaaa cctacactaa agtatggaat
     3301 tgccattatc aatccaccta gtataaatgg aattacagcc gatttaaata tagatgaaag
     3361 tgttaagcct atttgtgcaa atataaaaga agcaaaaata gtagtcaata taacaattat
     3421 atacatttgt attactgtaa gatttaaatt actatttcca aagtaccata agttatgaat
     3481 cggttcattt aaatttttaa aatcaaaaaa actaaaacct ctaattccta ctaacaatat
     3541 aaaagaaata ttaataatag ataaaattaa tccattagct actagttttg ataatactac
     3601 tgattttttt ccctttttag ttgtatttat taaacctgtc atattagaat catattcttt
     3661 aatgtaattt ccagaagtta ttataaacat tatcaaacct attagaggta ttgagccttc
     3721 tatcttgtaa aatatatctg cataagtatc taaaacatca aactttctaa tcagaagaaa
     3781 tcccaaaaat attaaaaaca gccaaattat ccaaacactc tttgatttta atatttttat
     3841 catttcagct ttaactaaat acatcatttc tactacacct cccttgcatc ttccaaatca
     3901 aaataaaaca tgtatacatc ttcgaatttt gcttgaactg gttgtatatt atccccactt
     3961 ggaatatcct ctgaaacaat tctaagtact actgaatcaa tatcactttg aatacttaca
     4021 accttatatt tagattgtat atcatcaact tcacattcat ttttaacttg aacattatat
     4081 actttatttt ccatgtgact taatatgtct ttgtgtgttc ctttcattag aagtttgcca
     4141 tctttaatca ttattgtttc ctttgcaact gattctatat cggaaattat atgagtagaa
     4201 agtattatta tcttatctct agacattttt gctattaaat tcctaaatct agctctttct
     4261 tgtgggtcaa tacctgctgt tggttcatca aagactatta tcttagggtc gtttagtaaa
     4321 acttgtgcta tcccaactct cctcttcata cctcctgaaa attttcctat agacttattt
     4381 ctcacatcgt aaagacctac taaatctaat aattctccta tcttactatt taaatatttt
     4441 ccccttaaac cttttaaagc tccaatatac tgtaaaaatt cttttgctgt aaaattttta
     4501 taaactccaa attcttgtgg caaatatcct aaaatatttc tataattagc atccatttta
     4561 aatatatcct caccattata aagtatctga ccttcatcag gttttatcaa cgttgttatt
     4621 tgtttcatta atgtagattt tcctgctcca tttggtccta gaagaccata aactccattt
     4681 tccagagtta ctgtaatctt gtcgtttgct acttttttac catatatctt tgaaagattt
     4741 tctatttcta atttattcat cttaactccc cttttatcta cttatataat ctgattttat
     4801 tactaaaaaa cataatgaca tagcacctat caatattgcc atcaaaagtc ctattgctat
     4861 attgacaatg tctatattca ctttaaatat cataatatca cctagataat ttcctattat
     4921 tttattaaaa ccaaatatca aatcataata atttacattt gatacaactg aaagtaattt
     4981 tttaggtaat atatggatta ccgacattat cttagttata gatataataa atataaaaaa
     5041 acttattgaa gatattgaag atttacttat aaaagaccca aatatggcta tcatggccca
     5101 tccttcaaac atcaaaagct ctatgcctat atttaatatt acatactttg ttatacttaa
     5161 attagttttc aatgcaacta cattgtctac tattctaaaa gattctaagt ctccattaac
     5221 aggtactcct tgttgcatac aagttataat aaatattccc attaaataaa cagaataaat
     5281 tactaatgga actaataaag caacaaatat tttagacttt aatgcttttt tcttctcttt
     5341 tgatgatagt atgatatctt ttaccgaagt aacgacttca tctacgtaaa tattggatat
     5401 aattatagat ataatgatta acattcctac atttataagt gaatttgtaa ctctatagtt
     5461 aaatactttc caaaaactaa tatccttata ttcattcaca cttagattat caagtttttt
     5521 ctttgacatt aattcaattt ttttagaaaa ttcacctgta tctgtttgat ttgaaaactg
     5581 ctttaaaaca ttaatcttat tttgaaattt ctcattacct atgtcgatac cacttctcgt
     5641 atcttctata catccttttt ctttatctat ataataacgt tctgtttcta aaacaggcgt
     5701 aataaagata ctaatcatta gtattaacac taatatccct agagatataa acatactctt
     5761 agatttagcc aattttttta tttcccaatt taccaatata tttctcctcc taaatcattt
     5821 taattacaca tatatattaa aacttaaatc ttatataaat ataatactaa ccttaaataa
     5881 agcttaaatt attttataat ttataggtgt gtttattatt ggagttacat ttattatttt
     5941 tctgggaaat tatacatttt caattatttt tattattttt gatattttta ttgattggaa
     6001 attttattgt tgttcaaatt gtagttattc tttaaattta aagatggagt tttttaagaa
     6061 tagaacctac tgtccttatt atggaaagat tattaaatag ttgcatataa ctttatttat
     6121 cttgaatctt atttttaaaa acaattataa ttttagaata ttttctattc tcaaacaagc
     6181 atgttgaata atgaagcatc ttctagtatt atatacttgt agaataaaac taaattggta
     6241 aaactgggaa tatttaacct taaggagtta agttatagaa actagtacta aaaaatttta
     6301 taaattatgt taattgacta atagaatgcg actttttatt tttactaaat tttttagggg
     6361 gatggataat atgtttattg gagaaataga caaatgtaca catatactaa ctgcttacat
     6421 cagtagttca tatgattatt gtaatttttt agatacacag ttagatgatt ttatatcaga
     6481 atatggagaa acggtagttg aaacctgctt acatcaagtc ttattattag taagtagata
     6541 caattaaaat taaatttttc tagaaaaacc tttttagatt tatctactct aataaatata
     6601 taattttttg tactttataa gtaactataa cttctgctaa agtaaagatg tattgggagt
     6661 acatctttac tttcccatta tagaagttaa aagcatacac cataaaatca atcatccaat
     6721 acaaattata tatttcctat gtctaactta atttacaaga atcattctat atgcatattc
     6781 tgcaatccac taaacatgct ctaaaccttt actattttat acattctcta taaatcatac
     6841 aaatcatata taaaatcccc ctaatatgtt taaaccacct ataaaataac accaatattt
     6901 agaatattca atatcaactg cataactggt caataaaaac gtataactag acaaatttca
     6961 atatttatat aaataataca agaaaaacaa gcattttatt tatataaatt ttttattttt
     7021 tgtttatatt aaaaaactat attgattaat tta
//
LOCUS       DAELSY010000181         6936 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.181, whole genome shotgun sequence.
ACCESSION   DAELSY010000181 DAELSY010000000
VERSION     DAELSY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.181"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..363
                     /locus_tag="KPG09_003395"
     CDS             <1..363
                     /locus_tag="KPG09_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0967958.1"
                     /translation="LMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGITL
                     FKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLATGNLNISL
                     KKTNAKLVPKINQKKEVL"
     gene            364..1119
                     /locus_tag="KPG09_003396"
     CDS             364..1119
                     /locus_tag="KPG09_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0967959.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTSPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            1250..2272
                     /locus_tag="KPG09_003397"
     CDS             1250..2272
                     /locus_tag="KPG09_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0967960.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRIGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(2436..2993)
                     /locus_tag="KPG09_003398"
     CDS             complement(2436..2993)
                     /locus_tag="KPG09_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG0967961.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2999..4075)
                     /locus_tag="KPG09_003399"
     CDS             complement(2999..4075)
                     /locus_tag="KPG09_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBG0967962.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(4076..4759)
                     /gene="modB"
                     /locus_tag="KPG09_003400"
     CDS             complement(4076..4759)
                     /gene="modB"
                     /locus_tag="KPG09_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBG0967963.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4908..5720)
                     /gene="modA"
                     /locus_tag="KPG09_003401"
     CDS             complement(4908..5720)
                     /gene="modA"
                     /locus_tag="KPG09_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0967964.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKTDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEVESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5913..6842
                     /locus_tag="KPG09_003402"
     CDS             5913..6842
                     /locus_tag="KPG09_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0967965.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
BASE COUNT         2398 a         1126 c          924 g         2488 t
ORIGIN      
        1 cttatgattt caaacggact tatagcttta tctggtagtc ttatggctca gttcttggga
       61 ttctcagatg taaatatggg tataggaact ttagttcttg gaattgcctc tatcataatt
      121 ggtattacac tatttaagaa atttactttt atcaaagata ctactgcaat aattgttggc
      181 tcttttatat accaattcac tatatacttt gcaatgagtt taggaatgtt atcaactgac
      241 ttaaaactaa taactgccat agttataata gctttcctag ctactggtaa cttaaatatt
      301 tcactaaaaa aaacaaatgc aaagctagta ccaaaaataa atcagaaaaa agaggtgttg
      361 tagatgttac aaattaaaaa tctttcaaag agtttcccta atccatatgg tgaacctaat
      421 actatctttg aaaatttatc aatcgatata gaagatggtg aatttgtaag tatcataggt
      481 agtaatggaa ctggtaaatc tactttatta aatataatat ccgggcttat aaaagaatct
      541 tctggtcaag tattattaaa taaacataac ttgtctaatt tagctgaaca cagaagaact
      601 caaatagtaa gtagagtttt tcaagaccca agtcttggaa cttgcccttc tatgactgtt
      661 agagaaaact tgtctttagc tcttaacaaa ggaaaactat taaatctaag aaaatgtctt
      721 cgttacaaaa gagatttctt agaaaattta ttggaaggtg tatctcttga tttaaaaaaa
      781 taccttgatg ttcaagtaaa atttttatca ggaggtcaaa gacaatcatt atcactaatt
      841 atgtcgtgct taacaagtcc atcagtttta ttacttgatg aacatacagc agcacttgac
      901 ccaaaaacat caaatgaagt aattgaattg actaataaga ttgtaaggga aaaaaatata
      961 actacactta tggttactca caacttaaaa cacgctcttc attatggtga tagattgata
     1021 atgcttcata agggtgaagt tgtattagat gtaaaaggta aagaaaaaga acaattaaca
     1081 gttgaagaaa tcttagaaaa atttgaatat gcagtttaaa ataatatatc tattgtatta
     1141 aactcggctt taaaataaag ttaaaattac aataattatg gttactttgt aataattcat
     1201 atgaatatat gaacatatat aataataaat ttttgagggg gatataaaaa tgattaataa
     1261 aaaaagattg gcaagtttga ttttagctgg tgctcttagt atatctatgc ttacagggtg
     1321 ttcacaagga ggagactctg gtaattcaaa acaagaaagt aattctaaag gtaaagaagt
     1381 taaaaaaatt ggtataactc aactggttga acatccagca ttagatgcca ctaggatagg
     1441 ttttgtaaaa gcattagaaa agaacggatt taaagatgga gaaaatattg atatagactt
     1501 ccaaaatgct caaaatgaca tgcctactac acaaagtatt gcgagtaaat ttgcatctga
     1561 taagaaggac ttaatatttg ctatatctac tccatcagct caggcagcat ttaatgctac
     1621 taaagatata cctatactta ttactgctgt gagtgaccct gttgctgctg gattagttaa
     1681 aactcttgaa aagccaggca caaatgtatc tggaacatca gattttgttt ctgtagacaa
     1741 aggtttagaa cttcttaaaa tatttgctcc taaggcaaaa actataggag ttatgtacaa
     1801 tactagtgaa gtaaattcca aagttcaagt tgatgcttta aaagaatatg catctaaaaa
     1861 tggatttaaa gttgttgaaa aaggtataac tacttcaaat gaagttaacc aaggtatttc
     1921 tagtttagtt ggcaaaatag acgtattata tgttcctaca gataacttag tggcttcttc
     1981 tatgcctata gtctctaaaa tagctactga gaataaaata cctgttatag cagctgaatc
     2041 tgggccagtt gaaaaaggtg ctcttgcttg tcaaggtatc aactacgaaa aactaggtta
     2101 caaaacaggt gaaatggcag ttaaaatttt aaatggcgaa tcagtatctg atatgcctgt
     2161 ggctacatca gatgatacag atatcattgt taatgaagat atattaaaag ctttaggtat
     2221 ggaaaaacct tctaatgaaa atatttctta tgtaaagact aaacaagaat agaaagatat
     2281 tttctatata cgattaatat gcctgtttct ataaattaat ttatagaaac aggcatatat
     2341 ttttatttta atgtattatc tatttaaaga ttatatgtaa atgctcttaa tatgattgta
     2401 taaattttac ttgtataaac tttatacaat catatttatt cttctatttt ctttattact
     2461 ctacatggat ttccaacagc tacagtattt ggtggtatat cttttgtgac tacacttcct
     2521 gcacctatta ctacattatc acctatagta attcctggtg ttatgattac tcccccacct
     2581 atccatacat tatcacctat ttttacagga gaaccatact ctatgcctga atttctaagt
     2641 tgtgcatcaa ttggatgata ggctgtatag atttgaacgt ttggagctaa cattacattg
     2701 tcacctattt caattttaca tacatctaaa aaaatacaat cataatttgc aaagaaattt
     2761 tcgcctacat gtatgttata tccataatca cacctaatgt tttgttctac atttatttgc
     2821 ttgccaactg aaccaaatag ttgtcttaaa atatcttctc ttttttcata ttcatcctct
     2881 aaagtattat taaacaatct agttaatttt ttacaatact ctctttcttt tactaacaat
     2941 tcatcatttg catagtatcc ttttccagac aacatttttt ctttttcagt catatagttt
     3001 actccttttt gttataattg tatctaaagc aaaataaata ttcctgtgga aaatctaaat
     3061 atattctgtc acctttctta aatctcattt tacttttttc caattctatt tgaataggta
     3121 cacattcgtt agataaatca gtttttctaa catatatagt atatgtaaaa ggattttcta
     3181 caatgttaat caattcgaaa cataactttt catttaaatc ttcattagaa tccaagacct
     3241 taatatgatg ctccctgatt cccacatatt ctatattatc acctatttct ctatttaatg
     3301 tcaactcgca tccccagtct gtagcataaa tagtattttt atccagtcta tttaatttgg
     3361 atatattttt acaccctgtt atcttagctt ctataaggta ctttggagat tcaaagagct
     3421 cttttttagg tctctttgga agaccttctc ctttgttgta cactataata tcatcacaaa
     3481 ctcggtaagc ttcttcaata tcatgagtaa caaatagtat atctcccttg taatctttta
     3541 acatattcat aagttctttt tccatattac ttcttaaatg atggtccagt gctgagaaag
     3601 gttcatctaa aagtaatata tctggagatg ttgccaatgc tctagctagt gctacccttt
     3661 gttgttgacc accagataat tgccaaggat atctcccttc aaacccattt aatttaagta
     3721 tatctaagta ccttgctact atttcctttt tttcactctt acttagtttt aaaagcccaa
     3781 gttctatatt ttgacttaca gtcatatgtg gaaacaaagc atagttttga aataaaaatc
     3841 ctacctttct atcttgtgtt gataaattta tcttcttttc agaatcaaat agaactctat
     3901 catttaatac aattttacct cttgttggtt tctcaagccc tgctatacat tttaatgtca
     3961 tactttttcc agaaccagat tcccccaaaa accccagagt acctttttct tgtttaattt
     4021 ctacctttag tttaaaagag gataaatctt tttcaatatc cacgtataag ctcaactatc
     4081 cacacctctt tcctataatt ttttgctgaa attccgacca ataatttagt aataaaatca
     4141 ttgctataga tattgctaca ataatcataa cccacatcat agctttattc atatctccgc
     4201 cttcaacagc aaaaaatata gctattggca tagtttgagt ttttccaggt atatttccag
     4261 ctatcataag tgtagcacca aattcgccaa gagctctggc aaacgacagc accaatccac
     4321 caattattcc aggccatgct aatggaactg caatcttaaa aaatactttc cattcattaa
     4381 gacctaatgt tctagcagct gacagcatat tattatcaat ctgttcaaaa gctgaccttg
     4441 aagttctata catcatagga aaagacacca ctactgctga aattacagtt gctgtccaac
     4501 taaatattaa agtcctatcg aaatttaata aaaactttcc tataaatcca ttcttcccac
     4561 atagtaataa taaaaagaaa cctacaactg ttggtggcaa aattagtgga agtgtaaata
     4621 gtccatctat aagacccttc cacttacctc tatagttagc cataatatat gacacaaata
     4681 ttcctattac aaaagttatt attgttgaca aaatagatgt cttaagtgat atccataagg
     4741 gtgaccaatc tgttcccatc tttccctcct taattttaaa tggaaatagt gaaactaata
     4801 actctattaa tttcactatt tccattttat tataatacac aattattttc aattttcatt
     4861 tatttatata taatttatac ttaattacac ataatttatt tataagttta ttctactttt
     4921 ttatatccaa attcttcaaa aatcttttgt cctgactcac ttaataaaaa ttcttcaaat
     4981 ttcttagcag catctacgtt tttactagct ttaattactg aaactggata tgtaataggt
     5041 gaatgagttt tttcgtctgt tttttcaact acttttatct tatcattatt gacagtatca
     5101 ctgaagtaaa cgaatccaac atcagcattt cctgattgca cccatgctaa aacttctttt
     5161 acatcttttg caaatactag cttatctttt aacttatctt ttaaatttaa attagttaac
     5221 acctcatctg catacttacc agctggaaca ctttcaactt caccaacagc aattttcttc
     5281 actttatctg tagtcaaatc tttcatacct gatacactac tgtcagcaga agaaataaga
     5341 actaaatcat tttttactaa atctttcata gtatcagaaa ctaataactt ctcttcatcc
     5401 aaagctttca tttgtttttg acctgctgaa ataaataaat cacaaggtgc tccttgttct
     5461 atttgttgtt gtaatgagcc tgatgctcca tagttaactg taagttttac atttgaatca
     5521 acttttttat attcttcttc aatctttgcc attgcttctt ttaaacttgc agcagccgat
     5581 atatttaact ctactgaatc actactttta gttgtttttt cttgttttcc atcatctgtt
     5641 ttgtcttcat tactattgca cccaactgtc cccaaagtta aacatgcaac taatcctaat
     5701 attcccaata tttttttcat aacaatctcc ctttttgtaa atttgataca cattttaata
     5761 tgttttatta tgttttatca tgttttgtta tatatagttt aattatatat acatgataat
     5821 aaattttcaa gtaatttatt tacactactt ttaaatattt tgctacattt tccttttttt
     5881 aatgttaaaa taaccttata tggaggtgga ttatggactc attatcatct ttgacacctg
     5941 tcgaggtagc tgaattatta aagataacaa aaaatactgt atatgaatta atcaaaagag
     6001 gtgaacttcc ttcttataaa gtagggaaaa aaataagagt tgatactaaa gatgttgaag
     6061 aatatatcaa cagccaaaaa actggtaaag taaaggatac acatttcaac aataaaaata
     6121 tagatataaa taataatttc aaaattatta tttctggtca agatgtaata ttagacattt
     6181 tagctaggtc tgttgaaaaa aaattggatg gaataagtac atttcgctct tatataggta
     6241 gttataatgc tttgtatgag ttatataatg gcagagtttc tatagcatca tcccatctat
     6301 gggatttcga aacggatgaa tataatagca cttttgttaa aaagctattg ccaggaatac
     6361 catgtgtact tataaatcta gcttatagaa tgcaaggttt ctatgttgct aaaggaaatc
     6421 ctaaagagat aacaacctgg gaggatttaa caaaaccaag tataaccatt gtaaataggg
     6481 aaaaaggttc tggaactagg gttttactag atggtaaact tagacttcta aactttaatg
     6541 gtaaacatat aaaaggttat gataatgaag aattatcaca tcttggagtt gcaagtactg
     6601 tttctcgtgg aatagctgat gttggtttgg gaaatgaaaa agctgcttta caagtaaata
     6661 atatcgactt tattcctctt cacaaagagc gctatgattt agttattaaa aaagaagact
     6721 tacaaaatcc agtttaccaa actataatta acataataaa ctctcctgag tttaaagctg
     6781 aacttcaagg tcttggcgga tatgatttaa ctgatactgg aaaaactata gcaaaagttt
     6841 aagattaaaa tcgcaatcta ttttataaga tttataattt ttcacaaaat ttataactta
     6901 aacacgaaat aaattgcgat tttagtgtct caaaaa
//
LOCUS       DAELSY010000182         6911 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.182, whole genome shotgun sequence.
ACCESSION   DAELSY010000182 DAELSY010000000
VERSION     DAELSY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.182"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(88..477)
                     /locus_tag="KPG09_003403"
     CDS             complement(88..477)
                     /locus_tag="KPG09_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0967966.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            complement(2066..3472)
                     /locus_tag="KPG09_003404"
     CDS             complement(2066..3472)
                     /locus_tag="KPG09_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0967967.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILKDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTIKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGVKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVAGIINAHRGKLDI
                     MSIDSQGLKTILKLPLIK"
     gene            complement(3517..4224)
                     /locus_tag="KPG09_003405"
     CDS             complement(3517..4224)
                     /locus_tag="KPG09_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0967968.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            4386..5309
                     /locus_tag="KPG09_003406"
     CDS             4386..5309
                     /locus_tag="KPG09_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967969.1"
                     /translation="MKHMEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGA
                     GKSTTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENM
                     TIMQRLLDLPKKNVNEALKIVRLENQKNKKVNQYSLGMKQRLGIAMAIMKFPKLLILD
                     EPTNGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQD
                     SIKELHNRGQSQIAIKAKNVVSAQKLLNTKGYMSLIQNGYLTIENLKDSDVAKINGML
                     VHSNIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            5309..6070
                     /locus_tag="KPG09_003407"
     CDS             5309..6070
                     /locus_tag="KPG09_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0967970.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPILMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            6082..6819
                     /locus_tag="KPG09_003408"
     CDS             6082..6819
                     /locus_tag="KPG09_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0967971.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         2357 a         1103 c          784 g         2667 t
ORIGIN      
        1 catttgtcat attacttaag tagactaata tattacataa atacacttac atacacttta
       61 tattaatgct ataagaaata cgtttgctta tacgtctttc tcctcttctt ttagattttt
      121 tatccactct tcaataagta agatatcatc tttgctcaaa ttaatatttt catgtaatga
      181 caacaataaa ctttttatag aagaatcatg aatatttcta aaaaaatctc ttgtctcaac
      241 tcctatgtat tccttttctt taattaaaac ttcataatgt gtatatttat ctatcttttg
      301 agaatttaga aatcttttta ctactaatct agataaaaga gtcaaagttg tcgtttgttt
      361 ccattgatat ttttgctcca ttcctaaaac aatatctcta gatgttactt tggaatctga
      421 tttccatata aatttcatta tctttaattc tgattgtggc aactttttta tagtcatgat
      481 aaaaccttgc tactcagaat atagacaagc aatatccata ataaaatttt tcgattaaat
      541 acatcttata cggatttata taagctcaga cttataagat atttttcaat cttaatccta
      601 aatttagcta tttgtacacg gtaaatatat ataaataatg tcaaaattct cttgtattat
      661 attgcttaat tttatgagat attttgtact cagctatcta ttttggatac ttgtccagta
      721 gctctccttt cttttaaact ctttgatttt aaatatttgt atttcagtaa atcctacttc
      781 ctaaattttt tatttactac aacttattac tgaacttcct acttcctaaa cttttcattt
      841 actacaactt attactgaac ttctcactta ctaaatttct tacttactat aaattataac
      901 taaacttctc acttactaaa tttcttactt actataaatt actactaaac ttcctacttt
      961 ctaaatttct tacttgctat aaattattac taaacttcct actttctaaa tttcttactt
     1021 actataaatt attactaaat ttcctacttt ctaaatttct tacttactac aaattattac
     1081 taaacttcct actccctaaa tttcttactt gctacaaatt attactacta aacttcctac
     1141 tccctaaatt tcttacttgc tacaaattat tactaaactt cctacttcct aaatttctta
     1201 cttgctacaa attattacta aacttcccac tttctaaatt tctcgcttac taccacttat
     1261 cactaaactt cctactttct aaatttcttg cttactacca cttatcacta aactttttac
     1321 ttcttaaatt tcttacttct taagaataat gttaatttta attttaagat tatagttttt
     1381 gccttgttta attgaaacat tctatataat tatatccttc tattttagag tttttttatt
     1441 tatatattac aaaatttaaa ttttttcatt aattatatta taaatttccc atatatttca
     1501 acataaatac atatatatat atgttatttc taaaaatact acacaaaaac tctactttag
     1561 tagaactcaa tggtgcaaaa tacttcaatt taaaacattt atcgtacttt aaataaataa
     1621 ttataataaa atatacttac aatagttaca tagaagctat tataactaca cagaacaacg
     1681 tataattaaa acttttttag gatgtcatta tattttttct taaaattaaa aaacactata
     1741 ccataattct attttagtag aatttttttg tcaataataa aattaaatat taactaaaaa
     1801 ataaaaaaaa tggaattatc taaagaattt aataacctta caataattcc aaaatattat
     1861 ttctatgaaa attttgatac catgagtaac tcagccataa gcgcataaag cacaagttac
     1921 tctttacttt aataaaactt gacaagaaaa gaatttatcc ttattctact ctaatagagt
     1981 tattttgtca ataaagaata aaattatttt caaaatattt tcattcttca ttcttcattc
     2041 ttatctgtta ttattttata aaatatcact taattagagg taattttagt attgttttta
     2101 gtccctgact atcaatactc ataatatcta atttacctct atgtgcatta ataattccag
     2161 ctactatcaa aatacctaga ccatgatttt gacctttggc atatgcctca cttagtccat
     2221 tttggctctt taataaacta ttaattcttt cttgactcat accttttcca tcatcttcaa
     2281 caattatata gcaaaaatct aaatcagact tagaggtaac ccttatatta catccttgtt
     2341 ggttatgagt tatactattt tgtattaaat tactaatagc cctttttaat aattttttat
     2401 ctgcatctat aataatatct tcatttagta tatcaagttc aacataaaag tcattatcca
     2461 aattattatt tataaactca gaaataagct ctctcaaaaa cgcagaaact ctaatcttat
     2521 cacaattcaa tgcttgcata ttatattcaa gtttagatac taaatttagg tcatttacta
     2581 attctctgat tttaacccct tgattgcata taatagacac ttgctcttgt tctctatttg
     2641 aaaggctact gctttcttca agttctccag catatccaat tatcattgaa agtggagttc
     2701 ttatatcatg agatattcct gcaatccagt tagacctagc ttcctctttc ttcctaagtt
     2761 tttcatcttt catttgcaac tctttagata cagtattaat agattttgaa atatcactta
     2821 gtattccttt ttcttgtaaa agtactggtt ctccttttga cataagcttt atgccagtaa
     2881 taattggttt tatagatttc accattttta aacctatgaa tagagctatt aaaaaagtaa
     2941 ctattgcatt aaccagaaac atacctatta ttaacatagg aaattgttct atttccttaa
     3001 tcgtataagt aaggttaaat ttagtgtatt tgtcctttgg ataacctgtt actaatattc
     3061 catttggatg ttcccataca tacacaggat agtcctttaa aaagaacctt gaaaactttg
     3121 ctacatctgt caaagtataa tgacttggaa tttcttttgg taaatcaaat ccccatattc
     3181 tatctccatt gttatcaatt aacattgccc aaactttatt tttatgtagt acttccttac
     3241 tagaatcatc taaaataaac tttccatctt ttaaaatcaa attatcttca atcataatca
     3301 agtttgattt tatttctctg ctatcacttg tatctaaatt atcagagtat ttagatacaa
     3361 atagcatata tgaaaacaga agaaaacctg ctatatttaa tacaataaaa agtaatgaaa
     3421 tcaagatact tgaacctata aatcgcctca atatgtactt tacactatcc aaaattaaat
     3481 cactcctctt gatttctttt attctaatat ctaaaattaa ttaatcatca acttatatcc
     3541 aagacctctt actgtaataa gatgtttagg tgtagatgga gttttttcta tcttctctct
     3601 aacccttcta acatgaacca taagggtatt ttcatatcca tacatatcat ctccccaaat
     3661 ggcttgacat agtgaatcat tagtaacaat tctcccttta ttttcataca actttaaaag
     3721 caacgaatgc tctttagctg ttaaagaaat ctcttgccca tctttctcaa cacatgcact
     3781 atttaaattc acaacaacag aatctcccag tttaaatact ggcaactcct cttcttctat
     3841 aggcacatat acacgattta atatacccac aagccttaaa gtaagttcct ttggtaaaaa
     3901 tggctttact atataatcgt cagcccctaa tcccaaacca atcaatctat cttcatcttc
     3961 acctcttgca gagagaaaaa taactggtat cttagatgtc tctctaattt ttctcataat
     4021 agagaaacca tctccatctg gcaatacaac atctaaaatt gcacaatctg gctttacatt
     4081 tctaaatacc ctaacagctt cttcataact tgatgctaaa aatactctac taaatccctc
     4141 ttttcttagg atttcatcta tcatttttaa taattccttg tggtcatcaa ccacaagtat
     4201 ttttttatct tttattgact ccataatcat caccctcctt tagtatacca tatacccatt
     4261 ttcacaactt tgcctaaata gacttttaag gttattgtaa ggtaaacttc agctaactga
     4321 aatgtaacca agttattata ctgatataag ttaaaagaat caataagcac agattaaaaa
     4381 aggggatgaa acatatggaa atgataatta gaacacaaag actttcaaag gaatacaata
     4441 aaatatttag agtaaaggat atagacctaa gagttccaca aggagctgta tatggttttt
     4501 tgggtcctaa tggagctgga aaatctacca ctcttaaaat gcttcttgga cttgctaaac
     4561 ccacacaagg aaatattaat attttaggta aagagttaaa tgaaaaaaat agaatatcaa
     4621 ttttaaaaga tataggttct ttgatagaat ctccatcata ctatggtcat cttacagggc
     4681 ttgaaaacat gaccataatg caaagactct tagatttacc taaaaaaaat gtaaatgaag
     4741 cattaaaaat tgtaaggtta gaaaatcaaa aaaataaaaa agttaatcaa tattctttag
     4801 gtatgaagca acgtcttggc attgctatgg caataatgaa gtttcccaaa ttattaatac
     4861 ttgatgaacc tacaaatgga ctcgacccag cagggattga ggaaattaga gaattaataa
     4921 aatctctacc agaaaaatac ggcatgactg ttctaatctc tagccatctt ttaagtgaaa
     4981 ttgaccaaat tgccacatca ataggaataa taaatcatgg cgaactggtt tttcaagata
     5041 gcataaaaga acttcataat agaggtcaaa gtcagattgc tatcaaagct aagaatgttg
     5101 tatctgcaca aaagctatta aatactaaag gatatatgtc acttatacaa aatgggtact
     5161 tgactattga aaatttaaaa gactcagatg tggctaaaat aaatggtatg cttgtccatt
     5221 caaatataga tgtatttaga atagaagaac ataaaaaaag cttggaagat atattcctag
     5281 aacttactgg taaggcggtg actttataat gaaatacctt ggtctagaat tttataaact
     5341 taaaagaaag aagattattc tgatgacctt tttatttgta gctgttgaaa taatgtggtg
     5401 tatcgtaaat tcaaataggg ctattacacg taatccagat atgttaggtg gatttgaata
     5461 ttcctatatg cttatgagtt ttgcaagttt aaatggtcta ttctttccta tcttaatatc
     5521 aataattaca tcacgcataa gcgatataga acataaagga gatacatgga agcttttaaa
     5581 atcttctgtg acatctttaa atagtattta tttatccaaa tttttatgtt ctgctatttt
     5641 ggtatctata cctatattga tgcaagtgtt atctattgtg ttgtttggac attttagagg
     5701 ggttattgag tcaatttctg tatctttact tttaaaatac acattaggaa caattcttgt
     5761 aagtattgct atcattgcac tacacctatg gattgcaact gtaattccaa atcaaatgat
     5821 tgcaattaca tttggcatga ttggtagttt tataggtttg acatcaggtt tgttttatca
     5881 aggtattcgt agattgttta tatggtctta ttacctagag ttatcacctt taagttatgc
     5941 ttatgataat gttttaggtt catctgtcta tagaattgat atgaattttt ctattacgat
     6001 tttagttttc ttaattggaa tgctaattta ttatattgga agaaatcatt tattaaaaaa
     6061 agaagtatag gagggattta aatgttaagg aagtcagttt tgggagaaat tctaaagtta
     6121 aaaaattctt ttatatggta tgtattatta gctttgccac ttataagtgt gttgattggt
     6181 tctggtaatt tttatctaaa tcaaggcata ctcaaaaaag aatggtacag cttatggact
     6241 caagttagtc ttttttatgg ggaatttttc tttcctatct taatcgcaat cttttgtgca
     6301 tatatttgta ggcttgaaca tatgaatcat aattggaata atgtaatgac actgccagtt
     6361 aatttaaaaa atatcttttt atctaaactt acagtagttt caatacttac agctattaca
     6421 caagttttct ttgttatctt ttatatagtt gcaggaaata tgtttggttt ttctggtagt
     6481 gtacctaaag aattgactgg ttggattgct aaaggttgga ttatttctat ttcaatagct
     6541 tctatacaat tatacatgtc tataaagatt agaagttttg caactcctat tggtattagt
     6601 ctttgccttt ctctatttgg tatgggatta atagtagcaa aacctgctgt tggtatattt
     6661 ttcccttatt ctatgttagg tagtgctatg ggggttataa agcaatctgc tttaaatgtt
     6721 aatgacacta tagttacaat atgtgttgca tttattacta ctatagtatt ttctgaaatt
     6781 gccaatagaa attttagaaa gaatgatgtt atttcttaat ggtaaatgct agatattttt
     6841 tggtttgaaa tttatattct ttttggtttg aaatttatat cccttttatt taaaaatata
     6901 tatcttttat t
//
LOCUS       DAELSY010000183         6789 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.183, whole genome shotgun sequence.
ACCESSION   DAELSY010000183 DAELSY010000000
VERSION     DAELSY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.183"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            105..1094
                     /locus_tag="KPG09_003409"
     CDS             105..1094
                     /locus_tag="KPG09_003409"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBG0967972.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEVHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            1166..1906
                     /locus_tag="KPG09_003410"
     CDS             1166..1906
                     /locus_tag="KPG09_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBG0967973.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            2106..3257
                     /locus_tag="KPG09_003411"
     CDS             2106..3257
                     /locus_tag="KPG09_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG0967974.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLEPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            3282..4037
                     /locus_tag="KPG09_003412"
     CDS             3282..4037
                     /locus_tag="KPG09_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBG0967975.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKNTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            4229..6616
                     /locus_tag="KPG09_003413"
     CDS             4229..6616
                     /locus_tag="KPG09_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBG0967976.1"
                     /translation="MKKFTSKKVARFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SDTYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSVKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMDGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVSYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFNIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
BASE COUNT         2735 a          700 c         1271 g         2083 t
ORIGIN      
        1 gatgtgactt ttttagaatg aaaaaccgta gtatattgat ataatagaaa taagacttaa
       61 aaatctgttg atagtattat ttataagtac agtaggagga taaaatgttt gaaaatttac
      121 ctaagataga tttacattgc cacttagatg gaagtgttag agtagaaacc atgcttgata
      181 tagctattaa agagaaaata gatttacctt caaataatat ggatgaaata aaaaaattag
      241 caaaggtttc ttttaattgt acttcactag atgaatattt agaaaaattt gatttaccct
      301 taaaagtaat gcaatctaag gaaaatttaa aaagaattac ttttgaactt ttggaagatg
      361 caagtagaga aaatgtaaaa tatattgaaa ttagatttgc accattactt cacacccaaa
      421 aaggtatgag tgttaaaaat ataattgagg gtataattga aggaattaga gaagctgaaa
      481 gtatttatga tataaaaggc aatttaatat taggttgtat gagaacaatg acctcaaaag
      541 aggcattatt agtaattgaa gaagggaaat catttgtaaa taaaggtgtt gtagcagtag
      601 acttgtgtgg tccagagaaa gagggatttt gtaaggaata taaagatgtt tttaaattag
      661 caagagaata tggatataaa gttacaatac atgcaggaga agctgcaagt ggagaaaatg
      721 tattggatgc tataaatatt ttaaaggcgg ataggattgg tcatggggta aaaattaaag
      781 accataaaaa ggcgtataat ttagttaaag ataaaaaaat attgcttgaa ttatgtccaa
      841 ctagtaatgt acaaactaag acagtagatt cttatgaggt acatccattt tatacttttt
      901 ataaagataa tttacatgta agtataaata ctgataatag aactgtatca gatataaatt
      961 taagttcaga actaaatgtt atatttgata cttttaaact aggattagaa gactataaaa
     1021 ttatttatag aaatgcagta gaagctagtt ttgcagataa agaaactaaa gagtatttga
     1081 atagtttaat ttaatagata aaaatattta aactttaaat taagctatta aattgacaaa
     1141 aatacattgt attaggagaa aaaatgtgaa agattatgaa gatgttgttt atattaaact
     1201 agtcaaaaaa gaacttgaag aatggaagaa aaatatatta aagaaacctt ctatatttga
     1261 caaagcatca aagggtatac agaataaatt taatggagta ttgccagata aatatcatga
     1321 aataattact tcttctataa aagtaatgac aaaaaccgta ctgtttggat ataaatatgc
     1381 tacaaagcca ccacttagaa atatgtcatt agaagaaaga gaaaaattag ttttagaaaa
     1441 agctaaaact tacaagacca cagcaatgat tgaaggggca ggaacagggg caggaggaat
     1501 cctcttaggt ttatctgatt ttcctttgct tttaggtatt aaagtgaaat ttttatatga
     1561 tgtagcttca atatatggat atgatgttaa agattataga gaaaggattt atatattaaa
     1621 catattacag ttggcttttt ctagtcaaca gcatataaac aaaactttta aattgatgaa
     1681 taactgggat gaatatgtaa atagtctacc agaagacatc aatgattttg attggagaac
     1741 atttcaacaa gagtatagag attatttaga tttagctaag tttttacaac taatgcctgg
     1801 aataggagca tttgtaggat tttatgtgaa tggaaaatta attgataaat tatgtgaaac
     1861 tgctgtgtct gcttatagat tgaggattcg agagcttaag ttataagaga aattataaca
     1921 taaaaaatta gttatacaat atatctatat gtatctaatt ttgcattgat aaaaacaatt
     1981 atggataaaa ggtaaagcat gatagaatct aatgtagagg tttgtatttt aatggaaaaa
     2041 tttagttttt attgcttaag atacaaattg aattacgaag agaagtaatt gggaggaata
     2101 ttttcatgac aaatttgcca aaacaatttg atagcttcaa tgaagctaga caaaagggat
     2161 ttataaatgc aaaggaactt aaggaaagtg ggaaaaagat ggttggagtt ttctgtacat
     2221 ttactccagt tgaaattcca atggcagctg gagcaactgt agtaggtgta tgcggtgtta
     2281 gtgaagagcc aatacctgat gcagaaaggg tgcttccaag aaatctttgt cctttaatta
     2341 aatcaagcta tggacatgca ataacagaca cctgtcctta tttctatttt tctgatttgt
     2401 tgataggaga aaccacttgt gatggtaaga aaaaaatgta tgaagaactt gcaaaagtta
     2461 agccaacata tgtaatgcat ttgccaaata cttcaaaagg agaatttgcc tataaacttt
     2521 ggaaagatga aatgataaga ttaaaagaag aggttgagaa atctttaggt gtaactataa
     2581 ctgaagaaga tataagaact gctataaaag ataaaaatga agaaagagaa cttttaaaag
     2641 agttttatgc acttggtaaa ttagaaccat ctgcacttac aggccttgag ttacataatg
     2701 ttttatatca agcaggtttt aaatttgacc gtgcagaact taaacattct ttaagaaaag
     2761 ttatagatga tatgaaagaa agatatgaaa aaggtgaatg tcctgttcaa aaagataagc
     2821 caagaatatt gataacaggt tctcctattg gtggaataag cgaaaaaata gttaaaactt
     2881 tagaagatgc aggggcatca gtcgttgcat atgaactttg tggagctatt agatcaaacg
     2941 atttattggt tgatgaagaa atagaagatg tatatgatgc tttaacacaa aaatatataa
     3001 atataggttg ttcttgtatg atgaataatg acaatcgtat tgaattatta gatagaataa
     3061 tagatgaata taatgtagat gctgtaatag atgtagtttt acaagcatgt catacattta
     3121 atatagaaag ttatagaata agagaatttg ttacaaaaga aaaaaataag ccatttatgt
     3181 ctcttgaaac tgattattca aaatcagata ctgaacaatt aagaactaga tttgaagcgt
     3241 ttgtagagat gttgtaggaa taaataaaag taggaggaac tatgtttagt ataggagttg
     3301 actcagggtc agtggcaaca aagggtgtgc tatttgatgg tgaaaaaatt attaagaaaa
     3361 taattatacc aactggatgg agccctaaaa atacatctaa acaggtctat gagttgctaa
     3421 gtagtgaaat tgacaaaaaa gatataaaaa aggtggtagg tacaggttat ggaaggggag
     3481 taatggattt tgctgataag aaagtaacag aaataacttg tcatactaga ggtatttact
     3541 ttttaaacaa gaacataagg actattttag atgtaggtgg tcaagatagt aaagttataa
     3601 atttagatag agatggaaat gtatttaact ttataatgaa tgataagtgt gctgcaggaa
     3661 ctggtcgatt tttagagatt acttctaacc ttttaggaag cgatattgaa agtatagata
     3721 cattagctaa aggatatgaa ccagttaata tatctagtat gtgtacagtt tttgctgagt
     3781 ctgaaattgt aagtcttcta gctcaaaaca tttctactgg agaggtagca gcagggatac
     3841 ttaaatctat tgcaaataaa tccacctcaa tgttagcaag aggtgaggta atagatgaag
     3901 ttgcatttac tggagggctt gcaaaaagta aagaactagt aaaaatgata gaagaaatac
     3961 tgggtaaaaa aatttttatt gcagaagata ctcaaataat aggagcttta ggagctgctg
     4021 taataggatt tagataaaaa catataaaaa gaaaatgaaa agctgaattt atcataaata
     4081 agttcagttt tttattttat tttgtttatt tataaaggaa aactttggta taattgattt
     4141 aaatgattgt atatttgtaa atacttgata tttagtcata tataacatta gaattttaaa
     4201 aattagctca aaataagggg gagaaagcgt gaaaaaattt acttcaaaaa aagtagcaag
     4261 gtttgttgca ctgtttttaa tatctatttt agtaatgtca acaattcctg tgtcagctga
     4321 caatagcaat acattgaatc agataaaaaa agcagaatac agtgatactt ataaacaata
     4381 tctaaatgat gctaaaaatg gacgtacaga aaaatataat ggaattatac caaatccata
     4441 tcagttagaa ggtactcaaa ttcaaagtaa aggaagaaca gctccaacat cttatgaccc
     4501 tagaaaatta ggtcttatga catcagtaaa aaatcaagag gatttaggaa tatgctggga
     4561 ttttgctgca atggcaacct tggaaagttt cttgaagtta aataattatg gagactacga
     4621 tttatcagaa gaacacttaa gatggtgggc ttcagatggt gaatatggat ggtctgtaaa
     4681 tgatatggat ggtgctttaa attatgaagc tatgggatat ttaacatctt ggtcaggtcc
     4741 taaattagaa aaagatatcc catacaatgg tagagtatct aaagcacaag gagcaaaaaa
     4801 accaactaat atgaatacag cacctactgt ttttaatgtg acagatgctg tgtgtgtaag
     4861 taatgacata aattctacaa aaaatgctat attacagtat ggagcagtaa catctggtta
     4921 ttatgaagat attaaatatc aaagtgatga ccaaaattca tattattgtc caacaaaaag
     4981 ttttaataca aatcatgcta tttcaattgt gggttgggat gataactatt ctaaagataa
     5041 ttttaatgca aatattagac catcaaaaaa tggtgcttgg ttaataaaaa atagctgggg
     5101 agattataat tctgagggtg gatatttctg gatatcttat gaagacaaga cacttatgtc
     5161 tgatatagat aatttttcaa tcaaaggtgg taaaaaacca aatgatgatg agaagatgta
     5221 tcaacatgac tatgcaagta ttgtaccttt aatttcaaaa aagataacag ctgcaaatgt
     5281 atttgatttc aatagaggcg atgaaactct aaaatctgta atgttcttaa ctgaaagtat
     5341 aggagctaag tatgaagtat cttatgctcc agtagtaaat ggtatacctc aagaaaacaa
     5401 tatgaaaaaa ttaaaagaag gtactgccca atattcagga tacataacag ttccaattga
     5461 ttcttttaat ataccagaag gaaagggtgc aattgtagtc agtatagagg ggaaaaatgg
     5521 agagtcaact ataggttctg agtcaaatgt accaggatat gatacattta aagcaaaagc
     5581 taatttaggt gaaagttata tcatagataa tactggaaaa ttctttgata taaatagaga
     5641 tagtaacttc tatccgtgta attttactat aaaagctgtt acagaaaagt cttctggtga
     5701 gagtatcccg aatgagtctt taattggtag tgatagatat gaaactgcta tcaaagttag
     5761 tcaaaatggt ttcaattctt cagaaaatgt agttttagta aatggcagtt caatagtaga
     5821 tgcattggca gcaactcctt ttacatctgc tataaattca ccaatattat tgacacaaaa
     5881 agaagcttta aattcaaaaa ctaaagctga gatacagaga ttaggtgcta agaaagtata
     5941 cctaataggc ggagaaaatt ctataagtaa agaaattgaa caacaattaa aaagcttaaa
     6001 tatatcgata gagagaatat caggaagtga tagatacaag acaagtttat tattagctca
     6061 aaagttaaat ggtataaaaa atgtatctca aatagcagtt gtaaatggag tgaaaggatt
     6121 agctgatgca attagtgtag gtgctgctgc agcagaaaat aacataccaa tcatacttgc
     6181 aaatgaaaag agcgaattgc aaggagctga tgaatttcta aattcattaa atatagagaa
     6241 atcttatatt attggaggaa cagcatccct atcaaacaat ctagaaagta agttaaaaaa
     6301 tccaactaga ttatctggta gtagtagaga cgaaactaat tcaaagataa tagataattt
     6361 ttataaaaaa gatactttaa aaaatgcatt tgtagttaaa aatggaatca agaatcaaaa
     6421 tgatttaata gatggattgg cagttggtcc tttaggagca aaaactgggt caccagtaat
     6481 tttagttggt gataaattag ctgatagcca aaaagaagtt cttaaaaata agactttaga
     6541 aaaagtaact caagttggtg gaggagcaaa taaaaatgca tttaaagagt tagtaaaatt
     6601 aaaaagtgct aaatagagta cttaagtatt aacatatatt agtgtgtatt agtaacttat
     6661 cttaataaaa gctgaaaata attactttaa attattgata aataagataa tcaatgattg
     6721 taaagttttg ttttcagctt taaatttatg tagtataagt ttaaaaacat aaaaaagggt
     6781 tactatata
//
LOCUS       DAELSY010000184         6750 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.184, whole genome shotgun sequence.
ACCESSION   DAELSY010000184 DAELSY010000000
VERSION     DAELSY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.184"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1265
                     /locus_tag="KPG09_003414"
     CDS             <1..1265
                     /locus_tag="KPG09_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0967977.1"
                     /translation="IKNKEKFRKDSMYILKNYYQDNFYLVQLDVNKFKYINEMFGYAE
                     GNKILIHISQVLNNNTNKYEICARMDNDHFILLIACNTEDELLNRLSKINKEICNLNT
                     TNSSKYKIVMSSGIYKITKKDDIKKIDLLIDRANIAAKSKKEKYEHSYSFFNEDTRNR
                     LYKEKRLEDNMNKALEKGEFIVYYQPKYSLDDVNEIEGAEALIRWNSPEFGFISPIDF
                     VPLFEKNGFIVNIDMFVFEEVCKTLNKWINKGYTPVPISVNMSRVHLYRDNFIENITD
                     LISKYNISPEFIELELTESVVFDNLNILIDIMKKIKEIGFLISMDDFGSGYSSLNLLK
                     DLSFDILKLDRGFLIETTDTKRGKIIISKIVEMAKAIDIKVICEGVETYEQVEFLKEI
                     GCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            1412..2158
                     /locus_tag="KPG09_003415"
     CDS             1412..2158
                     /locus_tag="KPG09_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0967978.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PTILLADEPTGNLDSKSTDDVLKLFTRINKEQNVTTLMVTHEVYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            2158..4038
                     /locus_tag="KPG09_003416"
     CDS             2158..4038
                     /locus_tag="KPG09_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0967979.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELTILGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEIYFPVKAILLTIVS
                     FMILFLITGPMSLKLLRKKSILELLVGTKKPKKEIKSSRLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYDEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFRLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            4157..5548
                     /locus_tag="KPG09_003417"
     CDS             4157..5548
                     /locus_tag="KPG09_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0967980.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            5655..6695
                     /locus_tag="KPG09_003418"
     CDS             5655..6695
                     /locus_tag="KPG09_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG0967981.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
BASE COUNT         2674 a          653 c         1131 g         2292 t
ORIGIN      
        1 gaataaaaaa taaagaaaag tttagaaaag attctatgta tattttaaag aattattatc
       61 aagataattt ttacttagta caattggatg tcaataaatt taaatatata aatgagatgt
      121 ttggatatgc tgaagggaat aagatattaa ttcatatatc acaggtttta aataataata
      181 caaataaata tgaaatttgt gctaggatgg ataatgacca ttttatattg ttgattgctt
      241 gtaatactga agatgaactt ttaaatagat tatctaaaat aaataaagaa atttgtaact
      301 taaatactac taactcatct aagtataaaa ttgttatgtc aagcggaata tataagatta
      361 ctaaaaagga tgatattaaa aagattgacc tgttaattga tagagcaaat attgcagcaa
      421 aaagtaaaaa ggaaaaatat gaacattcat attcgttttt taatgaagat acaagaaatc
      481 gattgtacaa ggaaaaacga ttggaagata atatgaataa agctttagaa aaaggtgaat
      541 ttatagtata ttatcaacct aagtatagtt tagatgatgt aaatgaaatt gaaggagcag
      601 aagccttgat aagatggaat agtccagaat ttgggtttat ttcacctata gattttgtac
      661 ctttatttga aaagaatggt tttattgtaa atattgatat gttcgtgttt gaagaagtat
      721 gcaaaacatt aaacaagtgg ataaataaag ggtatactcc tgtaccaatc tctgtaaata
      781 tgtctagagt tcatttatat agggataatt ttatagaaaa tataactgat ttgataagta
      841 aatacaatat atctcctgag tttattgaac ttgaactaac agaaagtgtt gtgtttgata
      901 atttaaatat attgattgat ataatgaaaa agattaaaga gataggattc cttatatcaa
      961 tggatgattt tggttcggga tattcatctt taaatctgtt aaaagacctt tcttttgata
     1021 ttttaaaatt agatagagga tttttaatcg aaacaacaga tacaaaacgt ggaaaaatta
     1081 ttatttctaa gattgtggaa atggcaaaag cgattgatat taaagtaatc tgtgagggcg
     1141 tagaaactta tgaacaagta gagttcttga aagagatagg ttgtgataag gttcaaggat
     1201 acttatttgc taaacctatg gtattagatg aatttgaaaa acacttaaac tttaaatttg
     1261 attaatcatt aataattatt agactataat ataccctaac aacttactta caaatatgta
     1321 agttaattgt tagggtattt ttatggttaa agtagtatat tcacgatatt atggtattgt
     1381 agtaaaaata aaaaattatg gagaatataa aatgaacata ttgagtatta gaaatatatc
     1441 aaaaacatat tgcggaaata ttccatttaa agcattagat aaggttagtc taaatataga
     1501 aaagggagaa tttgtgtctg ttatgggacc atctggaagt ggtaaatcta ctcttttaaa
     1561 tataatatct acagttgata gacagagtga aggtgaggtt gtattagatg gatatgatgt
     1621 aagtaaattg aaaggagaaa aacttgctga atttagaagg aaacaacttg gttttgtatt
     1681 tcaggatttt aatttgattg acactttaac tgttggcgaa aatattatgt tgccacttac
     1741 actagagggg gcaagtatca aagatatgaa tatacaaact aaaagtatat ctaaattttt
     1801 aggaatagat aaaattttag atagaaaaac ctatgaaatt tcaggaggtc aagctcaaag
     1861 gtgtgctatt gctagagcta ttataaataa acctaccata cttcttgcag atgagccaac
     1921 tggaaatctt gattcaaaat ctactgatga tgttttgaaa ttatttacta ggataaacaa
     1981 agaacaaaat gtaacaacac ttatggtaac tcatgaagta tatagtgcaa gttattcaga
     2041 tagagtaatt tttataaaag atggttgtat atatacagaa ataaaaaaat ctgaaagcag
     2101 taattctttc tattctgata tactagctgt tttgtctcaa ataggaggtg taagatagtg
     2161 gattttagaa agtttgctta taataatata gttagaaatt tcagggcata tttagcatat
     2221 tttttaagtt ctgttttttc tataatgata ttttttgttt ttgcagtgtc tatgttccat
     2281 ccgattataa caaaatctgg aatgcagagt ggttctactg catttatggc tcttatgact
     2341 tcagaattaa caatattagg ttttagtcta ctttttatat tgtactcttt aggaaatttc
     2401 ttgaaatata gattgaaaga ttttggagta ttgatgataa ttggaatgag caacagacaa
     2461 ctgaaaaaat tgatattaat tgaaaatttg ataataggat ttttagcagt gatgttagga
     2521 atattacttg gtttatcaat ttcaaaattg tttttattgt atttaagcaa actgttttat
     2581 atgaatttaa ctgaaattta ttttccagta aaagcaatac tattgactat tgtatcattt
     2641 atgattttat ttttgataac aggaccaatg agcttaaaat tattgagaaa aaagagtatt
     2701 ttagagcttt tagtaggtac aaaaaagcct aaaaaagaga taaaatcttc aaggctattt
     2761 ggaataattg gtgtaatatg tcttatatta ggatatgcta tgatactatt tggcaataag
     2821 ttaaaaataa atggaggtat gtatacaaca gcactattaa ttactttggg gatattttta
     2881 tttttttctc aatttactat tttgatatta aagtatttaa aaaataaaga aaaattttat
     2941 aagaacaaag taaatatgtt acttataagt aatttgatat ataaattaaa ggataataca
     3001 cgattgttat ttttaataac aatactttta tcggctactt tagtagcatt tacaacaact
     3061 tcaacacttg taagttctca aggagaagct tctaaaaata atttcccaat ggtttactca
     3121 tatttttcag agcataataa tgaaaaagaa tatgaagaac tacaacaaat tagagaagca
     3181 atcaaaggat atgattataa ggagttaagt tttcctgtac ttaaatataa tagggaagtc
     3241 ttactaagtg taaagaatta taacaaatta tcaaaggaat tgggattaga taaaattagt
     3301 tcaaagttag aaaaatatga agggattata attcctagta gcaattcaca agaacatata
     3361 aattctttaa aacaattaaa aaattatagt ataaaaaatt taaatataga agtaaaaaaa
     3421 tcaaaaaata aaataatttt tcctacagga ctgtttagta aagttgtagt tataagtgat
     3481 gacttatacg atgaagtaaa aggtgaattt tcaaaaatta attttcatgg atttgattat
     3541 aaaaactggc aaagcagtgc aaagataact gaaaatttaa aagaaaaaca ttttaatttg
     3601 gatagagaat ataacaggtc atatttttta acattaccag atatgtattt agtagagtta
     3661 caacaaagta aaattcttca atttatgggt gtttttatag gagtgatatt atttatagca
     3721 gtttttagtt ttttatactt cagattatat acagacgaga tagaagataa tgtaaagtac
     3781 aaaaaactat caaaaatagg tctatctttt aaaaatatga ataagattgt tagtatagag
     3841 ataggtacat tgttttttat accgtatttt atagcaatta taaatagcat aatttcttta
     3901 ctgtttttaa atcaagtatt aaataatagt tttaggttag aaaatttggg tatattacta
     3961 ctgatatcat ctatatattc actttatttt tacttattaa aaaaaattta tactagaaaa
     4021 gtttggtttt ttgattaaag tggtatataa tagatatata aattaatatt taaaaagtag
     4081 ttaatattag gggagggcat ttgcacctcc cttgtaatat gtggaaaatt gaatattaga
     4141 agaaggagga gacttgatgg aaaatcaaca attattagga acagaacgaa ttagtaaatt
     4201 actactaaaa tattctatac cagctataat aggtatgttg gtaaatagtt tatataatgt
     4261 agtagataga atttttattg gaaatatacc aggggttgga ccacttgcaa taacaggtct
     4321 tggtgtaaca atgccaataa tgacaataat acttgcattt gggatgttga taggtatagg
     4381 aactactact accatatcaa taaaacttgg gcaaggtaaa gtagaagagg caagaaaact
     4441 tataggaaat gcaatgacgt tatctgtaat aacaggaatt atcataatga tacttggaat
     4501 attatttgca aataaaatac ttacattgtt tggagcaagt gaaaatactt taatatatgc
     4561 aaaatcatat ataaatatta tattattagg tacagtagta aatcttcttt ccttttcttt
     4621 aaaccactca ataagagctg atggtagtcc taaaatttca gcaggaataa tgatagtagg
     4681 atgtttaact aatatagttt tagactggat attaatattt ggattcaatt tgggaataca
     4741 aggagctgca attgctacag taacttctca ggctcttaca gctattttaa ctatagggta
     4801 ttatataagt ggaaaatcaa acttacgatt cagtaaatct aatctaaaat tagataaaaa
     4861 gctaataaaa gcagtgtttg caataggtat gtcgccattt gcgatgcaac ttgcagcaag
     4921 tttagttcaa gtaatatcaa atattgcact taaaactcat ggtggagatt tagctatagg
     4981 ggctatggct actatttctt ccattgcaat ggtatttttg atgcctatct ttggaataaa
     5041 tcaaggagcg caacctatta taggtttcaa ttatggagct gaaaaatatg atagggttaa
     5101 aaaagcatat ttaggttcat tagtagtagc tactataatt ttatgtatgg gtatggtagt
     5161 agttatgctt ttcccagaag ctattatagg tatatttaat aaagaccctg aacttatgaa
     5221 tatatctgtt aatggattaa gaatatactt acttatgttg ccaattgtag gcttatcagt
     5281 tacaggaaca aactttatac aatctatagg aaaagcaaaa atggctatgt tattgagcct
     5341 tttaagacaa gtaattttat tgataccagc agtattaatt cttcctacat tcttaggttt
     5401 acagggtgta tggacagctc aaccagtgtc tgattttata gctacagtaa ttacaggtat
     5461 agttgtattt agagaattaa aaagatatac tcctaaaact gaaaaattaa atgaaaatga
     5521 aagactaaat gagataacta ctgagtaaaa atataaattc agataattga gcaagaaatt
     5581 gcaaaaaata cgattatatg ataaaataaa accgctgtca aggataagag aaattctaat
     5641 tatggagtga ttttatggaa tttacaaaga aaagcaaatt aaaaaatatg tatgtaaaca
     5701 tgaatgtagc tagaatgttg tctaagataa atgagtataa gggtaggcaa ctgctttaca
     5761 aaaaacaacc aaaagaaatt ttagaaaacc tagaaaaaaa atctttagta gattgttctg
     5821 aatctacata cattggaaat caaagagata gtagtaattt taatctagaa aagttgataa
     5881 gtaatgaagt tacacctaga agtagagagg aattgagtat tgttgaatac agagatgtgg
     5941 taaaaactat taacagtgct tatgagagta ttcccatcag ttcacaaacg attcttgagc
     6001 ttcatgggta cttatataaa ttttcatcta ctagaggagg aagttataag tcagataatg
     6061 attttattga acacaattta aaacctagcg aatttataag tattgattca agtgtcaata
     6121 aagttgaaaa agctgtagaa gaaatatgtg aagcttataa tacattgata gaggaagatg
     6181 aaattgatat attaatatta atttctgcat ttgtgttaga tttcatattg atacatccat
     6241 tcaaagaagg taatataaaa atggcgagag tacttatcct attgttacta aataaaaatg
     6301 ggtatgaagt tggaagatat ataagtttag gaaagatatt tgatgatagt tcatatgaat
     6361 attatagtaa tttaaattat ttgaaagcct caataggtag tgaaaaagca gatatgaatg
     6421 catggattga gtatttttta gagacgatat taactgcata tgagaagctg gatgatagtt
     6481 taaatatatc tgataagaaa agacaaacaa aaactagtag aatagaaaaa ataataaatt
     6541 ctacccttgg atattttaca aaagaagata taagagattt atgtcctgac attccagaac
     6601 ctactataaa tagagttttt aataatctaa gaaaacaaga taagatagaa gttgttgcaa
     6661 gaggaagaag tgcaaaatgg aagaaaaaat attaaaaaat gtttaaataa ctaaaatggt
     6721 atagtgtttt acactatacc atttttaatg
//
LOCUS       DAELSY010000185         6744 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.185, whole genome shotgun sequence.
ACCESSION   DAELSY010000185 DAELSY010000000
VERSION     DAELSY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.185"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(451..2838)
                     /gene="cadA"
                     /locus_tag="KPG09_003419"
     CDS             complement(451..2838)
                     /gene="cadA"
                     /locus_tag="KPG09_003419"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBG0967982.1"
                     /translation="MEANNLIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLIVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENASSKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFKEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELVPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGESSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKFNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKAIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2861..3226)
                     /locus_tag="KPG09_003420"
     CDS             complement(2861..3226)
                     /locus_tag="KPG09_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0967983.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3425..4273
                     /locus_tag="KPG09_003421"
     CDS             3425..4273
                     /locus_tag="KPG09_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967984.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMSNNMNSNMGMPNNMPNNMNSNM
                     GMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
     gene            complement(4711..5799)
                     /locus_tag="KPG09_003422"
     CDS             complement(4711..5799)
                     /locus_tag="KPG09_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967985.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLIIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKELMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5924..6652
                     /locus_tag="KPG09_003423"
     CDS             5924..6652
                     /locus_tag="KPG09_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG0967986.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
BASE COUNT         2364 a         1119 c          730 g         2531 t
ORIGIN      
        1 actatatcac cttaaaaata tttcgtcaat acttttttaa attatttttt aaaaatattt
       61 actttcttat attacataat ttattgaatg acagtcatac tgttggttta gagtatttta
      121 acacacacat tttgttgtaa aaattataac tattaatata atatatatac aatcataagt
      181 attataattt ttatgaatct aatttaaact taatatagac ttattaataa accttctatg
      241 tctaaatttt aaaattacaa tataaaagct ctacactaaa taaatacatt aaataaacac
      301 aatggataat gaatataaat ttatatatgc aaagcgctcc aataaaaagg taccaataaa
      361 gttaaaagaa acttattgat acctttcatt attacatact tcaatatata attataagtt
      421 ttttaaatag tatggattta catttatccc ttatctcata gctctcatag catttactac
      481 tgctataagt gccacaccta catcagcaaa tatagcttcc cacatagtag ctactcctcc
      541 agcacctagt atcataacta taatttttac tcctagagca aatattatgt tttgccacac
      601 tatcttatta gtcttgtttg ctatttcaat tgctttagat atcttgctag gctcatctgt
      661 cataagcact acatctgcag cttcaatagc agcatctgaa cctaagcctc ccatagctat
      721 accaacatca gcacgagcta atactggagc atcatttatt ccatcaccta caaatgctat
      781 tttctctttt tcagttcttc cttcatataa atcttctaat ctatctacct tttcattagg
      841 aagtaaattt gagtaaactg tatccaattc taattcttga gcaatatttt ttgctacttt
      901 ctcattatct ccagttaaca ttacaacttc ttttacccct attgctttta agcttcttat
      961 ggcattttta ctatcttctt ttacttcatc agatataact atataacctc tatatacttt
     1021 atcaacagca atatagacta ctgtgcctac ttctttagct gatgaataag atatattttc
     1081 ttttttcata agtttctcat tacctgctaa tataaaattg ccattatgct ttactctaat
     1141 tccataagct gctatttcct cataactgtc tattgtatct aaatcaattt ttttattata
     1201 gtaacttaat atagactttg ctattggatg atttgaattt acctccgcaa tagctgcata
     1261 ttctaataat tcttcttctg atataccttc aggatttaac ttagttacat tgaacacacc
     1321 tttagtaagt gttcctgttt tatcaaatac tactgtatct acacttctta aagcttctaa
     1381 atagttactt cctttaataa ggataccatt tttagatgca aacccaattc cactaaagaa
     1441 actaagtggt attgataaaa ctaatgcaca agggcaagaa acgactaaga atatcaatcc
     1501 tctatataac caatcactaa atacctcacc tgagattaca agtggtggca caaatgctat
     1561 taataaagca gctattacta ctattggagt atagtatctt gaaaacttag atataaagtt
     1621 ttctgtcttt gattttttaa tgctagaatt ttcaacaagg tcaagaatct tagaaactgt
     1681 tgattctcca aaactctttg taacttcaat actcaataaa gcatttttat ttataacacc
     1741 tgataagatt tcatctcctt tttcaacttc tctcagcact gactctcctg tgagtgcaga
     1801 agtatctagc atagaaactc catccactac tacaccatct aatggtactt tttcaccagg
     1861 ttttactact attatatccc ctacatttac ttcttctggt gacactacct taacttcaga
     1921 attaaatttt agatttgcat aatctggtct tatttgcata agtgaagtta tagactttct
     1981 ggatttacca actgcaacac cttgtaaata ctctcctact ttatagaaaa gcataacccc
     2041 tactgcttct gatgactccc ctattgttaa tgctcctact gttgctagag ccatcaaaaa
     2101 gttctcatca aatactcttc cttttgatat atttcttatt gctcttaaaa gcacatcgcc
     2161 acctactatt acataggcaa ctataaatac tatattgcta aactgacttt cataacctgt
     2221 agctgtttga tatataccaa atatatatac aagtgctcca attataagtg gaactaattc
     2281 tttcttattt gtatcattta cagctcctga tttttcagta gttctccctt ttactttgtc
     2341 agtagaccct acttgtatat caagtcctgg ctctgttgaa tttattattt cttttatttt
     2401 atttataaca tcatcctctt taaagttatt acttatattt actgtaagtt ttttatttac
     2461 aaagtttaaa ttagaagatt caacttcttt taatttagaa accttgttat taatctcctc
     2521 tgcacaatgt gcacagttta atcctcctaa tataagctct tttttaattg atgttttact
     2581 agatgcattt tctttagaac ttatttgtat atcaagacct ggttctgttg aatctattat
     2641 atctattatc ttttctatag tagtatcctc attaaagctt tcctttatat ttactataag
     2701 ttttttattt ataaaattta agttggaaga tttaacttct tgtagtttag aaactttatt
     2761 attaatctcc tctgcacaat gtgcacagtt taatcctcct agtataaatt cttttttaat
     2821 taaattattt gcttccatat atattcactc ctctcattta tcatttataa gtttctctaa
     2881 tatgatttaa tccacaatca aatatctggc ttatatgact atcatctaat gagtaataca
     2941 ctgtttttcc ttctcttcta aactttacta atctagcctg ctttagtact ctaagttgat
     3001 gagatattgc agagtgagtc atatttaata aactcgctat atcacataca cacatttcat
     3061 ttgcaaataa tgcatataat atctttactc tagtagtatc cccaaatact ttaaataatt
     3121 ctgctaaatc atataacatc tcttcatctg gcatagttga tttagcctct gttactattt
     3181 cttcatgtac tatattacaa ttacaatcat ttatctcttc tctcatcttt aatcctccat
     3241 tgttttaata tatgaataac tgttcatatg ttcattatat gctatcattc ctaaatttgc
     3301 aacaataatt tttattaatt taatataatt agaatgcaca tattataaac ttttttaata
     3361 tatatattat tagaataagt ttttgtaaac ttttttatag ttttctagga ggtgcaagtt
     3421 taatatgagt aacaagaaaa aaaaggattt agatactagt tacatgcctt acttagaccc
     3481 agcgaactat gcagattata ttactaatga agaaattccc caagatgaat tgagaaatga
     3541 ccctcaaatt tctaatggtt atacaaaaat acctaataac cagaacataa ataacctata
     3601 tcctaataat gtaaatcatt atcctaaaag tagtaattct ccaaacaact tgcctaacac
     3661 aaataataca tccggaaata tgaattacaa tacatctgct acaaataata tgcctaataa
     3721 catgaataat aatacgggta tgcctaataa tatgtctaac aatatgaata gtaatatggg
     3781 tatgcctaac aatatgccta ataacatgaa tagtaacatg ggtatgccta acaatatgtc
     3841 taacaatatg aatagtaata tgggtatgcc taacaatatg cctaataaca tgaatagtaa
     3901 catgggtatg cctaacaata tgcctaataa tatgaatagt aacatgggta tgcctaacaa
     3961 tatgcctaat aatatgaata gtaacatggg tatgcctaac aatgtgccta ataacatgaa
     4021 cagtaatatg gggtataata tgaataatac tatgggtatt cctaacttct catgtaatca
     4081 aaatatgcct cctaatgtac ttatgatgat accaggagtt atatgtcata atacaatgca
     4141 aggtatgcct gtagttatgc ctagtactat gcctcctaat atatatccta ctccttatgg
     4201 ttctagtaat atgtctatac aaggtatacc acaggctact aatattgaag aatttgatga
     4261 ggaagaaatg taatatatat ttttttgagc aagttgtata aaacattaat aattgaccat
     4321 aataataaat gtattcctca taatattccc ccaatattat tataggtata gaaagttgac
     4381 tgagtatgcg cttattcggt caacagatat taacataatc taataagata aacatagaaa
     4441 aagtcagata aaatttaatt ttatctgact ttttcattta aaaaactctt tcttgtgttg
     4501 gtgggtcaga atagtatggt actcgtattt gagactaata ttggcaaaca acataaagaa
     4561 aagtataaat tttttataat aaactgtata aaattttaat aattgaccac aataataaat
     4621 gtattcctca taatattccc ccaatattat agaaataaat ttaagcgaga ttaaattcag
     4681 ttaatctcgc ttaaataatg tctaaattta ttattccaaa aaatcatcaa aatctaatga
     4741 acctgattct gcaaagtcat ataatgcatt ttgctcaaag aaaaatttct cttcaaattt
     4801 aatatagtca ctataattag actgacttat ttcattgata taattgcaat cattttcgtt
     4861 ataagtacca ataaaaaact tatttcccat taagtaatat atagctttca tatcattgtt
     4921 tattatatat acattttctt ttacatggtc attattaaaa ttaaatctag taaaaaatat
     4981 accttcattc atattacttt ttaacagaaa tggattttct ctataaaata ctcctgcaaa
     5041 ttcttcaggt gaatttacac tgtaaacact tacaaattca cttttagaaa tctcatcaaa
     5101 tacctcaaaa tcatacataa gttctttatc tgtcactaat agagaattaa ttctaaaacc
     5161 agtttcatag cacttactta tgcaaaaagc aattttacat atataatatc catcattgtc
     5221 ttcatataaa gcttcactta tatatctgcc ttgaccttta tcacttacca cgtttttaag
     5281 taatgttcca tttatattag taatatgcat atttgcaatc tcgtcacttc cagaaaaata
     5341 ttttaaactt tctctatccc atgctataaa tctcatagtc atatagttta taaattcaac
     5401 ttcctcatca actcttttac atattttatt gtataatttt tctataatta aatctgtttt
     5461 atagtttttt ataatatcaa tatactcaca tttatctcct ggcaatagct tatcataata
     5521 aagattctta ttaccaaaat aagatactaa aaataaagat tcatccttcg ttaattcaac
     5581 tctatctgaa ccaaatcctc ccatcaatct ttcttcttcc atataagctt cttcttgtgt
     5641 tgggttattt aaactcacat aatctgctaa tcctaatccc tctgcatcta ataaaaaata
     5701 ttgacaaaag tgattgtttt catcatctat ccaatttatt ataagaccta aactgcccat
     5761 aagtctagaa tttgtagcct ttgcaaaatt aaaattcata tagtcacatc ctttaaactc
     5821 taaaatactt gttttttaat atataaatta ttatacatat attttaacaa cttagataca
     5881 atttataata taatataagg aaatgattaa ttgaggtggt cttatgaaaa atatagcagc
     5941 tttttttgat atagatggta ctctttatag agactctctt atggttgaac attttaaaaa
     6001 attaattaaa tacgatataa ttgaccaaaa aaattggtat aaacatgcta gagacacttt
     6061 tatggattgg gataaaagac aaggtaatta tgatgattat ctattagaaa tatgtgacct
     6121 ttatgttgat tcactaaaag gattagataa aacctgtatc aactttacaa gtgaccaagt
     6181 aattaaatta aaagcagata gagtttataa atacacacgt tcaaggattg agtggcatct
     6241 aaatcaagga cacatagtta tatttatatc tggtagccct ggatttcttg ttgaaaaaat
     6301 ggcaaaaaaa tataatgtaa ccgattttct aggaagtgat tatgtctttg aaaataatat
     6361 atttacaggc acagttattc caatgtggga ttctataagt aagaataatg ctataaatga
     6421 ttttgttgta aagtatgatt tagatttatc aaactcgtat gcctatggag atactaatgg
     6481 tgatataaat atgctaaaaa gagttggaaa tccaatagca ataaatccaa caaaggaact
     6541 tttatctcaa atatcacttg atgaggaaat ttctaaaaat gttgagataa tagtagaaag
     6601 aaaagatatt atttattctc tatctacaga tgtaaatata gtggatattt aatttatact
     6661 atctatttta aaaacttata ttatatctaa tatttatatc taacatataa aagctttgac
     6721 taaaaatcaa agcttttata tgtt
//
LOCUS       DAELSY010000186         6718 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.186, whole genome shotgun sequence.
ACCESSION   DAELSY010000186 DAELSY010000000
VERSION     DAELSY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.186"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(40..525)
                     /locus_tag="KPG09_003424"
     CDS             complement(40..525)
                     /locus_tag="KPG09_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0967987.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            777..2000
                     /locus_tag="KPG09_003425"
     CDS             777..2000
                     /locus_tag="KPG09_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG0967988.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTKESEPEKPIAVSVQKAVGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNANNKFKAGMSAQVNLSIEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            2004..5138
                     /locus_tag="KPG09_003426"
     CDS             2004..5138
                     /locus_tag="KPG09_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBG0967989.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            5172..6470
                     /locus_tag="KPG09_003427"
     CDS             5172..6470
                     /locus_tag="KPG09_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBG0967990.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINNVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNQDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
BASE COUNT         2632 a          884 c         1149 g         2053 t
ORIGIN      
        1 agttttaact tttttaatag tgacttttac aatcacactc taacctaatt tctttactat
       61 ttctgataac tttaatgtat gttcatctaa taaagttagt tcctcttcag aaagattaga
      121 taattttttt actagagatt ctttgagaat agcttttact gcatcaaata atttaaatgc
      181 tttatctgtt aattcaaccc taagaatcct tctatctttt gggtcaggaa atttattgac
      241 taaaccatgt tccactaatc tgtcaataat cggagtcata tttgatttta aaattcctaa
      301 attagaagct atttgagaaa ttgatgatga atttgatgtc ataagataaa gaattacctt
      361 agcatgtgat ggagccattg gacattcttc aaaataagct ttcattatat cagagttatt
      421 ttgaaacttt tttaacaaat cgctttcttt aaatatatta ttttcaagca caaataaaaa
      481 atctaaaaaa gagtccgcta ttttaagtat attgttgtta tccatatttg taacatccct
      541 tcaatagata atataataaa acattgttgt ttaatatagt ataaattata aactattaat
      601 aaatttaaaa caagaaaaag ttattgactc ataataatta taaatttata atcaaattag
      661 agaattataa attgccaata aagggaagtt ttttaattag tctaaaacta aacttaaatt
      721 ataagacact ttaaattcta caacaagcta gtgtgcaata tataatagga ggagatatga
      781 aaagatattt aatagttttc ttaatgtgta gttcactgtt tttagttgga tgtggaaaaa
      841 caaaagaatc tgaacctgag aaaccaatag cagtttctgt tcaaaaagca gtaggtggag
      901 aaattgaaaa cacaaattct ttctcaggaa ctacaaaagt taaagatgaa acagctgtta
      961 ctgcacagac tgttggcaca gttcaagaag tgtatgtaaa acttgggcaa aatgtaagaa
     1021 aaggagatga attattgagt ataagcagtc cagagctaga aaatagtgta aagcaaagta
     1081 aagcctcact agatttagca aaagcaagtt attcaagtgc cactggtggt tcattagagg
     1141 cacaagtaaa tcaggcaaaa acagctcttg acaatgcaaa gattcaatat gaggagtctc
     1201 aaagaaatta tgacaataat aagattcttt atgaacagga agtcattagt ttagaccaat
     1261 ttaaaaaaat agagttttct ttagagcaaa caaaacaaca gttagattct gctcaaagag
     1321 catacgatac agcaacttca aaaagtatac ctcaagctaa agcacttgct aaaaaacagc
     1381 ttgaccaagc tcaagtatca tataatttag caatgagtaa tttagacaaa ttaacattaa
     1441 catctccagt agatggtaca ataacagcta aaaactttga ttcaaaggaa atgataacac
     1501 aatcacaacc agcttttatt atttctaatc ctaatatact agaagttgat ttaaatgtag
     1561 cagaatcaga tataggtaaa tttaagaaag atggaaatgt agatgttata attgaagacc
     1621 aaaggatttt aggaaaaata gattatgtac cttcagtagt tgaccctcag acatctttat
     1681 atcctgttaa agtacttgta aataatgcaa acaacaaatt taaggcaggt atgtctgcac
     1741 aagtaaactt aagtattgaa aaagaaaatg gtgctgttac agtgcctaaa aaagcaatat
     1801 ttgaagaaaa tggtaaaaaa tatgtataca tagcaacaaa agacaatata gcaaagaaac
     1861 acttggttca aactggaatt gtaacagagg ataaaataga aattaaaagt ggtgtaagtg
     1921 ataaagatac agtgattatt ggaggaataa gtcttatttc tgacggaact aagatatttc
     1981 ctgtagaaaa ggagaagtag actatgaatt taacacagac ttccgttaaa cgtcctctca
     2041 caatcataat ggtcttttta gtattaatag tttttggtgg tattggatat aaaaaaatgt
     2101 caataaatct aatgcctgat atagaaatac cagttgtaat ggtcatgact acttggacag
     2161 gtgctggtcc tcaagacgtt gatgaacaag ttagtcaaaa agttgatgaa agtttatctg
     2221 ccgtttcaaa tgtaaaatct actatatcaa gttcacaaga aagtgtttct atggtagttg
     2281 ctcagtttga atttggaact aatttggatg aaataatgaa tgatgttagg tctaaagttg
     2341 atgcattaca gaccagcctc ccagatgatg cagcaaaacc aacagtttta aagctggata
     2401 tgaatgcaca ggctataggt caacttgtca tatctggagg aaatgaaaat agttcacaag
     2461 ccttaagaaa atacgctgaa gatgtaattc agcctaaaat agaatccata gatggtgtta
     2521 cttcagcaga ccttaaaggt ggagaaaaag cacaggttaa tgtaatagct gacccagctg
     2581 ttctttcaaa ttatggagtt agtttatcca ctataaaagg tgtcttatcc tctagtaata
     2641 aaacattccc ttatggttca attactcaag gtgaggataa aatagtttta agagcaattg
     2701 ataaattaga atccctagat gatataaaac agattcaaat tcctgtaaaa ggaggaaata
     2761 cagttagatt agataatctt tgtagtgtag attatggata tgcagacaaa gatagtattt
     2821 atagatacaa tggaaaagac agtttggtaa tagatatttc taaacaacaa gatgccaaca
     2881 ctgtaaaagt tatgcagagt gcatacaaat acgtagatga attgaataag gaaaatcctc
     2941 aatttaaaat taaaattgct aatgatacaa gttcatacat aagtgaatca ataaacagtg
     3001 taatgagcaa cttgcttata agtgctgtta tcgcatttat tgtaatattt gctttcctaa
     3061 aaagtgtaag agcttcacta gttgttgcag ttgcaatacc aatttctata ataggtgcta
     3121 tagcaattct ctactttact ggtgaaagtt taaatcttat tactgcaagt tcattggtaa
     3181 tttcagttgg tatggttgtt gataatgcta cagttgtaat tgaaaatata tttaaatata
     3241 ggaaaagtgg cagattgagt cttgatgatt gtgctattga gggaacaaga actgtaacaa
     3301 atgcaatact tgcatcaaca ttgaccacga tagcaatatt cttacctatc atgtttacag
     3361 aaggtatagc taaaataact tttggggcat taggtaaaac actaatagct tcactagcat
     3421 tctcctttat aggtgcaatt acactcgttc ctagtgtatt tgcaaaatta agcagaggaa
     3481 aaaatagtca aaagatgcaa gaaaaagata gtcctatatt tgataaggta agtgaagcat
     3541 ataaaaaatt attaagtgta tgtttaagac atcaacgctt agttgtaata tcaagtttac
     3601 ttatttttgt agcttcctta tttggaatgg gatttatagg tatggacttt atgcctgcag
     3661 cagacaaggg tgaattatct atttctatag aacttccaaa aggtcttagc ctagaatcca
     3721 atgattacta tgtatctatg actgagaaaa aagtctcaga tattccagaa attaaaactt
     3781 taataacaac tttgacatca gattcaaata atagtaaagc aagtatagca attgaattag
     3841 aatctcaaaa aaatagaaaa agaactactg atgaaataga aaaagagata attgatagag
     3901 tggctactgt accagattgt aaaataaatg taagtcaaaa cagtagtgtt atgggtggag
     3961 ctggaggagc agacttcaca ttagaaatga aaggtcctga tttagatgta ttaaaaatac
     4021 tgggaaaaga agctgaaaaa gaatttagct ctataaaagg atttagaaat gttgaaactt
     4081 ctcttacaga tacaacacaa gaagctcaat ttactataga taaacataag gctcaagaat
     4141 atggtgtaaa tactgctgaa attgcaggca tgttacgtac agcaataaat ggtgactccg
     4201 taaccactgc tactattgat gattacaaag tggatgtaaa cctaaaattt aaagcagata
     4261 ctattgatag cttagaagat ataaagcaac taaaagtaaa ctctgcaact ggaaaagaag
     4321 ttcctcttgg tgctttttca gatataaaaa tggcagatgg tctacaatct atatctagga
     4381 ctgatggaga tttttctgta aatataactg ctacacttga caatttagat gctggtactg
     4441 ctacaaaact agcactagaa aaaacgaata aaatggtaat tcctaaagat tactctatag
     4501 gtgttggtgg tgaaacagaa atgatgaatg aatctatggc aagtcttgga ttttcaatgg
     4561 tcatagctat agcattagtt tatatggtaa tggtagctca gtttgaatca tttagtaaac
     4621 catttataat catgttctgt gttccatttg cattcgttgg agttgtgctt tcgcttctag
     4681 ttagtggtca aagtttaagt actgtcggta tgctaggtat aatcttactt atcggtattg
     4741 ttgttaataa tggtatagtt cttattgact acatagagca acttagaaaa acaaatcgta
     4801 acaatgattt aattggtttg gtttcaattg gttgtgcagc aagattaaga ccagtcttaa
     4861 tgactacagc aacaacaatt ttaggtatgt tgccaacagc actatctctt ggtgaaggtg
     4921 gagaaactat gcaaccacta gccatagtta taatgggtgg tctaactgta tctacactta
     4981 taactttggt gcttatacct actatatact taatatttga taaatgggaa aataagtttt
     5041 cagacaaaat gcctaaaata tttggtaaga taacagataa aataggaagt ataaaacgta
     5101 aaataataaa gaaaccaaaa ttgaaagcta aagtttaaat agaatggagg gtgattgtat
     5161 tgaataaaaa aatgagcaaa atagttgcaa ttggctgtgg tgttggtata ataactgcca
     5221 atttaatccc agtttacgca gataataata cttcttctat aaacaacgtt gataattcta
     5281 ttttaagtga ctcaaataaa agaacatctg aaaaggaaat tttgactgct agagaagctg
     5341 ttagtgctgc acttaaaaat agtgaaaaat taaaaatgaa atcagaagaa ataaaaatgt
     5401 taaaagaaaa gttagaagta caagatgagt ttgatagttt tactggaagt gacaactcgt
     5461 ttccatacga ccaaattgga cttcttaaaa atcaaagtga acaggctaaa ggctttatgg
     5521 aggaccaaat agcaaatgat attactaata aatttaacga tttagtttca agagaaaatg
     5581 agctagataa gataaaaaac aatcttgaaa taaaaactaa agaaattaaa gatatgaaat
     5641 taaaaaaaga tttagggtta gtaacatctt tagaaactga atcagctgaa cttgaacttc
     5701 aaacactaca aaatactcaa aaagctaaac ttcaagaact aaaaaacaat caagactatt
     5761 ttaagttatt gactaatata gatttaaata attatcaatt ggataaagaa tatagatttg
     5821 aatcttttag agtaggtggc tcagttgatt catatatgga aggtaaggtt aatgaatatt
     5881 taaaatacga ccaattgatt ttagaacgta cagaagaaag ctttaatgat aaagatgaga
     5941 acaaagcaga tttaccagat agacctaatc ttacaagacc agttgctcct acaaaaccag
     6001 agcaaggtaa cctaactgat gatgaataca aggttcttat ggataagtat gaaaaagact
     6061 acaagagata tgaaaaagaa gttgaagcat acaatcttga aagacagact tatgctgctg
     6121 gccttactac ttatgcaaat tatcttcaac aaaaatttaa taccgaaaat ggtctagtaa
     6181 cattagaaga cagtaaaaaa gctcttaaaa aagggttaat tgatagctat gctcaattac
     6241 ttgctttaga agatacaatt cagattacta aaaaacaact tgatttatca gaaaaacaat
     6301 taaaaaatac taaattaaga tatgatttag gattaattac tctaactgat tataaaaaac
     6361 aagtggtaag taatcaagat gctaaaaata gctatgacac tcttattgtg aattataact
     6421 cactaaaaaa tggtatagaa aaaccttgga tacttaatac tggaaaataa ttctggtaaa
     6481 tagaaagatt actttaacat acgtttgatt aaaaaatagt tatgcacact cgtggcttta
     6541 gtcatggaag attcagaagg caaataatag aaatagatta gtaaaaatat atttacttat
     6601 aaaactgtat acttttatag aggctatctc aaaattggat atgtccctgt caagtagaca
     6661 gacttaaaaa aggctatctt tatgcggaat gagttcgata ttcaatcgaa ctcattcc
//
LOCUS       DAELSY010000187         6716 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.187, whole genome shotgun sequence.
ACCESSION   DAELSY010000187 DAELSY010000000
VERSION     DAELSY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.187"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(535..1203)
                     /locus_tag="KPG09_003428"
     CDS             complement(535..1203)
                     /locus_tag="KPG09_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG0967991.1"
                     /translation="MPVLMAIGVPVHLAIGTNKFAASAGCISSAYRYAKSGKINNDLL
                     KKLVPFTIIGSVLGVRCVLSISEEILNVLVVVMILIVAIYTFISKNLGQEDNFEAVNK
                     KNLRLGMLMALIMGFYDGFFGPGTGTFLTFGFIKIYGYDFLHASANTKILNLTSNITS
                     LLLFMINGQVDYKIAIVFALVMIMGAYVGAKVAIKKGSKMIKPIFLVMALFMVVKLVY
                     QTLV"
     gene            complement(1148..1288)
                     /locus_tag="KPG09_003429"
     CDS             complement(1148..1288)
                     /locus_tag="KPG09_003429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0967992.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGTYKHACSYGNWSASTSCHW
                     YK"
     gene            complement(1347..3002)
                     /locus_tag="KPG09_003430"
     CDS             complement(1347..3002)
                     /locus_tag="KPG09_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0967993.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQMIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKGI
                     FETKKENKFCDICKNIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVDSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(3231..3599)
                     /locus_tag="KPG09_003431"
     CDS             complement(3231..3599)
                     /locus_tag="KPG09_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0967994.1"
                     /translation="MKYSITDVSKITKISSSAIRFYEEKGLLLPINRKPSGVRYFSEE
                     DVERLIFIKELKNTGLSIKDIHECFHFCDMGDGTIDKRIQLFQQHKQNIKNKIHELES
                     CMDKIDEKIYYLKEKKMCKK"
     gene            3748..4542
                     /locus_tag="KPG09_003432"
     CDS             3748..4542
                     /locus_tag="KPG09_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase"
                     /protein_id="HBG0967995.1"
                     /translation="MEKYATMIKEKLNEGKVLCGVAVSLSDSVVSELIGISGYDFIWI
                     EGEHSALDRKDIQQHMIAAHAGGAASLVRLVNSDPDLVKPIMDMGTDIICFPFINTVE
                     DAQKAVAACTYPPNGIRGYNPQRASYYGNMNYFDYVKNANNETLKMMLIEQKAGYDNL
                     EEIVKVEGIDIIALGPGDLSLDMGFSGYMNRIEIKDMISHAAEICKKYKMPFIIFPTI
                     EKKSIEEWVNIGANMLCFAQDTTFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(4695..5123)
                     /locus_tag="KPG09_003433"
     CDS             complement(4695..5123)
                     /locus_tag="KPG09_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG0967996.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(5599..6537)
                     /locus_tag="KPG09_003434"
     CDS             complement(5599..6537)
                     /locus_tag="KPG09_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG0967997.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         2271 a         1109 c          661 g         2675 t
ORIGIN      
        1 aaaaattaat ttagcataac cttgttaaat atagatttga atatgaattt aaagttatgt
       61 aaaattaaaa atacaggaat gaaaattcca ttcctgtata aaatctaact tttgagtctc
      121 aatacaatct tattttgaga caatcttttt tgttttatat tttcttatat ttaaaatata
      181 acgcatatct tatgcaacat attcatattt ttctttatat tctttcatcg catcattcca
      241 agcgtcttgt actgttacac ctcttttatt agcaattttt tgtgccagtt caattatctt
      301 catagctctt cttgatatgt tcataaacat tcctcctaca aattaccata tatttcatat
      361 atataaatca aggttatatt tttatgatat ataatccttc tattgtaata ttacaattta
      421 tatgtgtatt atatatgaac tatattagaa gataattaga atttttataa agctatatga
      481 ttagattaaa tataaaagct ttacaattac ttataaattt aaaaagtcta taaactaaac
      541 taatgtttga tataccaatt ttacgaccat aaataatgcc attactaaaa atattggttt
      601 tatcatcttt gaacctttct ttatagcaac tttagcacct acataagctc ccattatcat
      661 cactaatgca aacactatag caattttata atctacttga ccatttatca tgaataataa
      721 caatgaagtt atattacttg ttaagtttaa tatttttgta tttgcagaag catgtaaaaa
      781 atcatatcca tatattttta taaatccaaa agtcaaaaaa gttcctgtac ctggaccaaa
      841 aaaaccatca taaaaaccca ttataagagc cataagcatc cctagtctta aatttttctt
      901 attaacagct tcaaaattat cttcttgacc taaattttta gaaataaagg tgtaaatagc
      961 cactattaaa atcataacca caaccaaaac atttaatatc tcttcactta ttgataatac
     1021 acatctcaca cctagaacac taccaataat agtaaatggt actaattttt ttaaaagatc
     1081 gttattaatt tttccagact ttgcatatct ataggcacta cttatacatc ctgctgatgc
     1141 tgcaaattta tttgtaccaa tggcaagatg tactggcact ccaattgcca taagaacagg
     1201 catgcttata agtccctcct cctcctgcta tagaatctac aaaagcagct aaaaaccccc
     1261 ctatacataa aaaaataact gaaaacataa aattcctcca tcattctctt tcaatttttc
     1321 ttacaatata tattatataa tcttatttaa tacttattat ccagcatatt ctttctatat
     1381 tcatttggag ttatactatt aaacttctta aatatttgtg aaaaataact actgttatta
     1441 aaacctactt tcttacaaat atcactaata ctcatgcttc tatctaacag atatttttta
     1501 gattcttcta tcctaagaag atttatatac tcagttattg aaactttatt tctttcatta
     1561 aaaatagaac tcaaataatt tggacttata aaaaaaacat ctgatatact attcaaattt
     1621 atattttctt tataatattt ttttatatat tcgtttatat tttcaattag atttccttta
     1681 tcttttctcc aaaatgctct attcttatca atacactctt tggaaaattc aaataataca
     1741 ttccataaat cttcatcact gtctacaagt gatatttttt tattatattt atttcttaaa
     1801 taagatataa gttttaaatc ctctaaatct ctaagcactg ttctagacaa tacaaatatc
     1861 atttcctgaa tagtatctct aacatcatta tatcttaatt tgtttgtatc tattagatta
     1921 taaacacctt ctaaattcat atttttaatt tctttttcaa tataaagttc taattcaaag
     1981 ggatattctt ttgaagaatt attgaacata tctatcggat taataatata ctcttcttct
     2041 aataatctac tatttgcttt tttaagtgcc tcttcaagtc ttatattttc ttttctactc
     2101 tttaatatct cctcatcttt tatactaagt tgattttcaa aaaaattcct cttagctatt
     2161 tcctgaataa attcagacat cattaataaa ttttctttta atacatataa tctactttta
     2221 ggatttaatg gaaattcatt gtaaattttg tttatgtatt ttatatcaat accagaacta
     2281 cttgataatt cagataaatt tttatctata tcaatagact tatatactat aaaattacca
     2341 cattctatat atccaactat attttcttct aatactatag gtatctctaa actggaaaca
     2401 ccatactcac aatggtacac tttttcaaag ttatcattat ttatgctttt aacaagtttt
     2461 ttagtacacg gttttatatt tttacaaata tcgcaaaatt tattttcttt cttagtctca
     2521 aatattcctt tatactttaa gtcatataca gatattctaa gcttcaatat attagatatt
     2581 tgatttgcaa gattttcaat tatgtttaca ttaactaact cttgtatatt gttttcagaa
     2641 aatactatag gtatttgtct caaactcgaa aatttatgag gaacataagt aattaatatt
     2701 actaaatcaa ttttaccagt gtttataatt tcatgttctg aatatggtgg gcaataaact
     2761 atagatgttt cctttatatt gaccttttta ccatcaatca tttgtattcc ttctccctga
     2821 atcacatata aaagctgttc ttcacttaaa tgaaaatgct tttcttggca gtttcccgga
     2881 aaaaatctta caattccagc actaagtcta ttgaattgtg tttttgatgg ttcgtgtatc
     2941 cacataacct ctccccaatc aaatttttgt acttcattat ttgactcaat aaaatccacc
     3001 ataaactcac ctcgtagttt ttatctaaat atttttttat tcctttaaat atctccttat
     3061 aatatacatt atcataattt tcttttttat tgtatagata aatagttata tagaacattg
     3121 tccaattctt cctaaaaata tagtgtaaaa aatctttcct caaatgtttc caattgaatt
     3181 tactctttat attagaaaaa tcaaaaaatt atttctttat tctataagga ttatttttta
     3241 cacatctttt tttcttttaa gtaatatatt ttctcatcta ttttatccat acaactttct
     3301 agttcatgta ttttattttt aatgttttgc ttatgttgtt gaaataattg gattctttta
     3361 tctattgtac catctcccat atcacaaaaa tgaaagcatt catggatatc tttaatagat
     3421 aaaccagtat tttttaattc tttgataaat attagacgct ctacatcttc ttcagagaaa
     3481 tagcgaacac cacttggttt tctattgata ggaagtaata aacctttttc ttcataaaat
     3541 cgaatagcag atgaagaaat ttttgttatt tttgatacat cagttataga atatttcata
     3601 atctctacct ccctaaattt tatatgaata agaatattat aaaaatacct tatacagtag
     3661 tcaacaaaaa agtattgact tcaagttagg ttgaagttgt actctttgaa gtgtaaaaga
     3721 aaacttacaa aagaaaggat gatataaatg gaaaaatatg caactatgat taaagaaaag
     3781 ctaaatgaag gaaaggtctt atgtggagta gctgtttcac tgtctgattc agttgtaagt
     3841 gagttaatcg gtatatctgg atatgatttt atttggattg aaggagaaca tagtgcttta
     3901 gaccgtaaag acattcagca acatatgata gctgcccatg ctggtggtgc tgcttctttg
     3961 gtacgattag taaacagtga ccctgacctt gttaagccca taatggatat gggaacagat
     4021 attatttgtt tcccatttat taatacagta gaagatgcac aaaaagccgt agcagcttgt
     4081 acttatcctc caaacggtat aagaggatat aatcctcaaa gagcctctta ttatgggaat
     4141 atgaattatt ttgactatgt caaaaacgca aacaatgaaa cattaaaaat gatgttaatt
     4201 gaacagaaag caggttatga taatcttgaa gaaatagtaa aagtcgaggg tattgatatt
     4261 atagcattag gccctggtga cttatcttta gacatgggtt tttccggata tatgaataga
     4321 attgaaataa aagatatgat ttctcatgct gctgaaatat gcaaaaaata taagatgccc
     4381 tttattattt ttcctacaat agaaaagaaa agtattgaag aatgggttaa tattggagca
     4441 aatatgcttt gttttgcaca agatactacc tttatcactt atgttatcaa tcaattatta
     4501 tctttttata atgagattgt accaattcaa aaacaacatt aaattataaa tctaatttta
     4561 tattaaaaga gactaatatt ttatctatct ctattaagga tattttctta tgtatcataa
     4621 tattagtctc tcttattatt atatactgct atcatcatta gttgctattt atactatttt
     4681 tataatatac aaatttattc agctaacgtt ttgcctaatt ctatacactc atcttttgct
     4741 tcttcatttg ggtcttccat tgctgtaatt ccttctccac caataattgt agcaccagca
     4801 ttttgcatac gttcttccca gtctctcatc cattcacaat ttccccatcc atatgaacca
     4861 aataatccaa tctgtttacc tgataccata gattctaatt ctgaaacaaa tggctccata
     4921 tacccctctt ctagttgctc tgctcccata gatgggcaac ctaatgcaaa tttatcttct
     4981 tcttttaaat cacttggttt cagtaagctc acatctaaaa cttctggtgt cttacctttt
     5041 aacttcacac cttcagccac aaaatttgcc attttttcag tatttcctgt tccactccaa
     5101 taaacaatat atattttact catttttacc tcctgtagta ttttttaatt aatcaatagt
     5161 tagttatagc taactaacat ctaaaaaaca aaagatttct ttatctgaaa tctctttatt
     5221 tttctaaata tagaatatca caattgattt tgaaattcaa tatttattga taatatttat
     5281 catttatatt ttatattcat tttataatat caaaacactg tatcgtattt ctaatctttc
     5341 atagaaaaat ttctaactta tagtactttt gcatctctta taaatctttt atatgtataa
     5401 gtacatagaa aaacacacct tttccagatg tgctatctat aatatcgtat atttattttt
     5461 caattctaac tagctaatat gtttataaat tttatcactt aataccttta tacattgatg
     5521 taaaatctta cacctaagta atttttatta tttgtctcga ctctatataa tatagctatt
     5581 ttatcatcaa atcaaaaatt atcttttgct attattcttg tatttataaa gatttaaata
     5641 taagtatcca tttccttgtt ccgttctagg tattccaagc gctctcgtac attttattaa
     5701 ttgtgcatac agttcagctt catctaaatc tctttcaaac tcctctcttg cccattcttt
     5761 aattagtgct aatgagcctg atacgtatgg tgcactcata ctagtaccac tcataatagc
     5821 aagtttatta tccatataag tcgatataat atttcttcct ggagccacca aatctattgt
     5881 agtatttgaa ttactaaact tttcaaccaa atagttttca tttattgcac ctacttctat
     5941 tacctcagca taactggctg gataactata ctcacttgta ctagaatcac catctccatt
     6001 attacctgct gcacacacta cagaaatatt attttttact gcttgcatga cagcattttt
     6061 taaattctta tcatctttgt ttcccccaag agacatagat ataatatcaa ccttgttatt
     6121 tacagcaaag ttaatagcat taattatact ttgatatgtt ccggtaccat ctttatttaa
     6181 tgcttttgct attaataatt tacaatctgg agctactccc ataacttcat tattatagtt
     6241 agatgcagct ataatacccg ccacatgagt tccatggcca ttaaaatcct catatatatt
     6301 tttatttcca ttactgtcat cactaaaatt tgcaccacca attatttttc cttttaaaag
     6361 aggatgagat atatcacaac ctgtatctat tataccaact acaatatttt taccagtata
     6421 accttcatcc cacattcccc tagcattcat ttgttttatt ccatatggga atttattttt
     6481 tgcacctctt agattttcat ttatttcata cggaattagt ttcattcttt tattcatcat
     6541 atatcctcct aaaaatttta ttgcttataa aaatcatgaa ttttatttta taataggttt
     6601 ataaatcaaa ttaaaaccta tatctactta tttatattta tgatatattt tttattttta
     6661 ggagtcatat ttttttgtat ataaaataat atttagataa ctatattaaa aaatac
//
LOCUS       DAELSY010000188         6704 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.188, whole genome shotgun sequence.
ACCESSION   DAELSY010000188 DAELSY010000000
VERSION     DAELSY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.188"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(24..632)
                     /locus_tag="KPG09_003435"
     CDS             complement(24..632)
                     /locus_tag="KPG09_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBG0967998.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(741..3479)
                     /locus_tag="KPG09_003436"
     CDS             complement(741..3479)
                     /locus_tag="KPG09_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBG0967999.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNRKFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3627..4418)
                     /locus_tag="KPG09_003437"
     CDS             complement(3627..4418)
                     /locus_tag="KPG09_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0968000.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4454..5110)
                     /locus_tag="KPG09_003438"
     CDS             complement(4454..5110)
                     /locus_tag="KPG09_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0968001.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5103..6068)
                     /locus_tag="KPG09_003439"
     CDS             complement(5103..6068)
                     /locus_tag="KPG09_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBG0968002.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEHVKQYLTSKGIRWEEMINE"
     regulatory      complement(6174..6280)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         2128 a         1135 c          614 g         2827 t
ORIGIN      
        1 acatatctta cctatataaa aacttattct atataaaaac gccataaaaa atcttttgct
       61 tcttctgcat aatctatatt tatacgttta ctagttttta tatctaactc atctttacta
      121 aatacttttt ttcctttatc atcataataa aaatcactta tatataattc ttcacccatt
      181 atactcttac tatttagact tctatctatt tttaaagctt tgcaaagttt tcctggtccg
      241 ttagtaatat tttttacttg atatttactc aactcagtgt aagctttgtt gtatctattc
      301 atagatatct catctaaaga agttataggc tctacccctc ttattaatac acactctggc
      361 acattttcta tatttgcaga taaatttaag caattataca taccataaat taaataaaca
      421 tatatatgtc caccatctaa atataatggc ttagttctat ctgtcttttt attgccatat
      481 acatgtgctc ctttatcatt tacacccatg tatgcttctg tctctatgat tttggtaaca
      541 attactttat tttcatattt ccttattaga tatttaccta atattgattt ggctaaatca
      601 attccatttt ttctaaaaaa atccttttcc aaaatttcac ctcataaaat ttatttaaaa
      661 cgcatcaaat acaactaaag taataaataa aattaactct tattaatgtg atatatataa
      721 acaataaatt tagccattta ttatttatat atatcaaaaa attctatctc aaacttttca
      781 atattatcat tgtaatttga attatcatat acttcttcaa caacctcttt aattgggaat
      841 ataactttta cattagttcc ttctcctacc ttactatcta tgtaaatctt cccattttga
      901 atttcaatca atgattttgt aaggcataaa cctataccac ttccaaattc atttgatata
      961 ccactgtttt caatttgttt aaatcgatta aatatatcat ttaatttttc tttgtctatg
     1021 cccactccag tatcttttat aattatctct actgtttcgt cttttttata tatacctaca
     1081 aggatttctc ctccttctaa agtaaatttt atagcatttg ataatatatt tagtattatt
     1141 ctttctatag attctgggtc aagccctacc ataacagttt cttcatccgt atcaaaaatt
     1201 aaatctattc ctttagactt agcataacta ctaatagatg taactattct ttctgttatg
     1261 tcaataatat caaaattaac tgctttaatc ttatatatgt ctgacttaac ttgagaaata
     1321 tttattatat tatctattaa tctcaataat ctaaacatat tttgtttagt tatttttata
     1381 tgttctttta gtatatcctg aaaatcaatt atttttcctt ttttataaag gttttcaata
     1441 agttgaactg aactatatat attatttaaa ggtgtcttaa gttcatgaga tatattagca
     1501 taaaactcca tcttcatttg gtcatactgt ctttgtttta ttaatgcttt tttaagtctt
     1561 tcagactctc tttgcacact taaatcttta ataattctca ctctacttct tttctgtcca
     1621 aagtataatg ttcttgatat catttttact tctttaatgt gattattttc atttactata
     1681 tggatactct tctctctttc tcctatattt aaattgttta tgtatttttt tactaatata
     1741 tttctatatt tttgtgttgt aactcctaat acatcactag aaattccgaa caactctcta
     1801 aaggctctat ttgtatatag tactcttgat gtacttaaat cctcaagaaa aatagcctct
     1861 ggtataacat caagtaattt ttcatatatt attttttgtt catttactgt tctaagaata
     1921 tttatctctt tagttatatc tctaaatgta agcaatttaa agagtttatt atctgaccaa
     1981 tagtctccaa ctctaacttc ttcaacatgt tcttcacctt ctgagtcaga catcacaaaa
     2041 ttaactatac agtttgctgt gtcattgtaa cttactattt cagctttctt ttcttgtgtt
     2101 agatttaatc ttttaaattt cctattgtca aagaaatcta tgttaaaagt ttcaaaatat
     2161 tgttctaatg tcatatcgac taaatcactt gaaacacatt taaaactctt ttcatatttt
     2221 ttatttatac ttattacctt attcttcttt atgccacctt cagcacttct tacaagtata
     2281 ggagatggaa tatttagtat aatgttttca tagtctttat ttatatttct tatttcttca
     2341 tctattttga tttcatttgt aatatcattt aaacttatta agtaattgct ttcatcttta
     2401 ttattactta aacttaacaa ataaatttta tcatttaact ctaccagttt atcaattata
     2461 cttatatcgt tatcaaattt cttcaatttt tcagcaactt tttcatcttc ctttataaaa
     2521 gttaatattt tataataatt attcatatcc tttgaaaatt catctttaaa tttattattt
     2581 gtgtaactta tattaaaatc atagtctgtt actatccata catttggtat tgtatccaga
     2641 gcttgtctaa gtaattcttt tctctggtct atgtagtttt ttaatgttct ttgtataatc
     2701 atatcctttt ctattgctaa atttatcttt cctaattgct ctgacttttt acttaatttt
     2761 ttattgattg aatcaagttc ataatatgga tacactactc tattattaag actgtataca
     2821 tatacataat aagtatggtt acataatagt aaatatatta ctcctattaa tctatctgaa
     2881 tctactatat taatcttaga aattgctaca aaacatataa tagttgtaaa aaaaacaaaa
     2941 tatgttaaat cttttattaa cttatagtgc tcaatgttac tatcaagttt attcaacaaa
     3001 tatagtaaat taaacataga tatacaaata ttaattaata taataatcat attccagtta
     3061 atatcactaa aaatatttct tataaagtaa tctaatatca caaaagcact ataaaaaatt
     3121 agtattgggc ttattcttag tttttttact ttagaaatgg ttaagtatat aaaagtagac
     3181 atattaatta tgcttaagtt aagcaataca atttggaatt tttgaatttt taaatttact
     3241 actcctaaaa tacttacaaa aactaataca agacacccta ctttcaatat atttataaat
     3301 tcatctgttt taaagctact tatataaact atagaatatc caaatatcca aagtaaaatt
     3361 attctataca tatttataga aactgtatac aaatttatat ttaaaagtgc aataaccaaa
     3421 aataacccat tagctagtat atagacctta ttcttactta aagttccttt agattccata
     3481 ttccccacca cttcttcatt tttatatagt tttacctata aatatgttac cacaattctt
     3541 ccaaaaaatt tgtcactttc tagttataaa aaaaaatgag taaatatttt taattttact
     3601 cattttgtag attttgtata ttcagtttaa aaagctggaa ctatacttcc tttatattca
     3661 tcttgtataa attttttaac ttctttacta ttcatagcct ctgataaagc cttaatctta
     3721 tcactatctt tattattttc tctacaagct attatattta catatggaga atcacttgat
     3781 tctattacta ttgcatcctt agtaggattt aagttagctg ttaaagcata attagaattt
     3841 attactgctc cgtcaacatc ttttaatact gttggtaatt gctctgcatt catttctttt
     3901 attactatat ttttaggatt tttagttata tcttttttag ttataagctc tccatctttt
     3961 acttctatca atttattttt tgctaataat ttaagtgctc tagctccatt agttgcatca
     4021 tttggcacag ctataacagc tccatctttt aattcatcta atgacttaac tttttctgaa
     4081 tagaatccca ttggctctat atgaacttta gatgtataag taagtttata tcctttctcc
     4141 ttcacagttt cctctaaaaa tggaatatgt tgaaagaagt tagcatccaa agacccttca
     4201 tctaaagctg tatttggaag tacataatca tcaaatattt ttacttctaa atcatatcct
     4261 ttttctttta acaaaggttt tgcaacttcc aatatctttg catgtggatt tgatgaagct
     4321 cctactagta tttttttatc ctctttgttt gaacaaccta ctgctgatat tgctattgct
     4381 gacaccaatg ctactgataa taatttcttt aatttcattt ttactccccc tctttaaaat
     4441 gtttataatt aatttatttt agtttcttat aagccagatt tcctactccc tggattatct
     4501 gaactaatat tataagtgct attacagtat ataccattac tgctgtatca aatctttgat
     4561 atccataaat aagtgctata ttacctaatc ctccagctcc tactgcacct gccatagctg
     4621 tataaccaag tatagatatt attgataatg taattccaga tactattgaa ggcatagctt
     4681 cctttatcat aactttaaat actatttgtc tttttgtagc tccaaaagat tttgctgctt
     4741 ctatcaatcc tttatcaact tcatttaatg agctttctat aattctagcg ataaacggtg
     4801 ctgccccaat agttattggc actattgcag cagtttcccc tatacttgtt ccaacgatta
     4861 atctagttat tggaatcatt gcaactataa gtatcataaa tgggaaactt ctaaatatat
     4921 ttactataaa tcctaatgca ctatttattg tgctatttgg ttttaatcca tctggttttg
     4981 taactactaa tatgattgct aatataaatc ctaatattgt tgccactata gttggtacta
     5041 taaccatata caaagtttgt aacaatgcat ttggaaatag tgttgttaaa aagtcaatta
     5101 aactattcat ttatcatttc ctcccatctt atgcccttac tagttagata ttgttttacg
     5161 tgctctccac ttttatctga aatgtttata ataagtgaac ctaaaatatc atctttaaat
     5221 tgttctaatt tcccaaatat tatagacaca tctatattta gttctcttgc catagtcgta
     5281 ataatagctt cattagaaat atctttaggg aatagtatct ttatatttgt tccttttggc
     5341 aatattatac tttcttcacc tattaatttt ctaagtcctt tagtatttct taagaatatt
     5401 tcttctgtat ctcctacctc taatacttct ccattttcca ttattgctac tcttccacaa
     5461 atctgcttta taacttccat ttgatgagtt acaactatta ttgttattcc caatttttta
     5521 tttatatctt caagcaatga aagaattgac tttgtagtat ttgggtctaa tgctgaagtt
     5581 gcttcatcac aaagaagtac ttgtgggttt agtgctaaag ctctagcaat tgcaaccctt
     5641 tgtttttgtc cgccacttag atttctgggt ttatcatttt tcttttcaga tattccaact
     5701 acttctaata actctaaaac tcttttttta atttctgcct ttgagtatcc aaaacattct
     5761 aatggcaatg ctacattatc aaatacagtt tttctttcaa gcaatgaaaa gtgttgaaaa
     5821 atcattccta actcttttct taaatctctc atttgctttt cattcaatga ctttacttct
     5881 ttatcagaaa caagtacact gccttcttgg tattcttcta atccgtttat acatctcagt
     5941 agtgttgatt tccctgcacc actatgacct attattccaa aaatttctcc agattctatt
     6001 tcaatactta catctttaag cacttgaatc tttccataat acttattaac attttttatg
     6061 ctaatcataa aatttcctcc catatattct gctcagtttt tatgtttatc ctctcttatg
     6121 tattacaaca aaataaaagc ccgagtataa actcaggctt agaaagctat ttatactcat
     6181 ctatcaacta tattagttgc aggatttagc accaatgtat ataaaatata ctggttgccg
     6241 ggtttcaaag ggccagtccc tccaccactc ttgataagac gttatttaat tcaattctat
     6301 tatcatttgt tgctatgtta tatatgatat attcattaat agtatttgtc aataaattta
     6361 tttaaatttt ttatttttta ttatcgttaa ttaactattt ttaattagtt ttctagctcc
     6421 taacacttta tattttaatg tctatataac tatagaacat cattataaga aataaattaa
     6481 ataacatcaa tctatctaaa tcatctgtaa agtaaattaa gaaaattaac attttatcca
     6541 aactctatcc tactattaaa taaaataatt taaatacaat gatgattata tagctctaat
     6601 actttagact ttctgaacct cccatgacta aagtcacagg gttcctgctt catagaattt
     6661 tgcatactaa aatatgtctt actaattctc cacaggctat cccc
//
LOCUS       DAELSY010000189         6573 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.189, whole genome shotgun sequence.
ACCESSION   DAELSY010000189 DAELSY010000000
VERSION     DAELSY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.189"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(204..713)
                     /locus_tag="KPG09_003440"
     CDS             complement(204..713)
                     /locus_tag="KPG09_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG0968003.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(715..1362)
                     /locus_tag="KPG09_003441"
     CDS             complement(715..1362)
                     /locus_tag="KPG09_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968004.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(1376..2548)
                     /locus_tag="KPG09_003442"
     CDS             complement(1376..2548)
                     /locus_tag="KPG09_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0968005.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNIESYTHCKESVNKLAE
                     RLKLEFEKEGLDCELVDVGENGNTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGALCAFNMETGLVDNSLCIGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSRENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            2816..4192
                     /locus_tag="KPG09_003443"
     CDS             2816..4192
                     /locus_tag="KPG09_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968006.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEILNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            complement(4443..5276)
                     /locus_tag="KPG09_003444"
     CDS             complement(4443..5276)
                     /locus_tag="KPG09_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0968007.1"
                     /translation="MTIKEVEEKTGLTRSNIRFYENENLIKPLRNERNGYREYSDENV
                     EDIKKIAYLRTLGITIDDIRNIILQKVSLVEVVEKQEKVLETKISDLENAKTMCNKML
                     ESDELSYLNLKVEQYITELPEYWNKNKPVFKLDSVSFLYIWGGKITWGIITIACLLIA
                     ITVFPKLPPQIPIQWNNGVANYVDKKFIYAYPVICVVFRILLRPFIWRWLQIHAFYSD
                     TIADYLTNFMCFVALSIEIFSILFIYNIARHVTIIIFIDILVFIVLLIIGVYKLFKKY
                     N"
     gene            complement(5605..6071)
                     /locus_tag="KPG09_003445"
                     /pseudo
     CDS             complement(5605..6071)
                     /locus_tag="KPG09_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816343.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
     gene            complement(6136..>6573)
                     /locus_tag="KPG09_003446"
     CDS             complement(6136..>6573)
                     /locus_tag="KPG09_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0968008.1"
                     /translation="LNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDL
                     QVKNMIRNRKLSRLISDVSWSEFIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKN
                     EEVKDLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         2262 a         1131 c          704 g         2476 t
ORIGIN      
        1 gaaattatga aaatatactt gatttaatac caaaagagta tctcatgaac taaaaagttc
       61 attttgagat actctcttct tttttaatta atatacatat taattactat attagtttat
      121 tactatatat caattactat tcattaatta ctattcatca attcttatat atcatttata
      181 attaggttat aattttatgt tatttacatt aatcccataa cagtgagtaa aactcttact
      241 atcaacatcc ccactattgc atcaaatata ggaactgcca acagtaacat tctatactta
      301 gtactggcat ttgctacaag cactattctg gcataatgac caactagagt acccattaac
      361 atacatggca taattaatat ggttgcttgt ggtgcagtga ttagaccttg tgcaaataag
      421 tttgcagctg tagctgctcc tgctgcctta gcaaagaatg ctgctatcaa aactacaaca
      481 gattccccag gtagtccaaa aatacccatt actggaccaa aaatatttcc taaaatatca
      541 actaaaccag ttaattgtaa aaactgcact attacataac ccattatcat ggctggtaat
      601 atctgttcaa cacctatgta aaaacctttt tttgctcccc ccataaatat ctcaacagca
      661 gttttcttag gtttactatt tacaattgca tttccagtat tttgttgact catactacac
      721 agcctcctta gccttattct ttttatttct tacacctatg attaatctaa ctagattagc
      781 accaagtatt ttacagaata tctctataag gattattgga cctaatgcca ataatgctat
      841 tggtaaaagt ggagcttgag tatttattgt gttcagtata acagctgaac cagcatattg
      901 atatgctaca aatatagttc tttcatcatc tgttatttcc ccactttcaa ataattcttt
      961 cgtcattatt gaggctacat ctgaactagt aaatgaagat acaaatgcta ttcctgctat
     1021 tccaggtata ccaagcagag gtctaagtaa tggattaaat atctttgatg ctgctttcat
     1081 tcctcccata gattcaacta catctataag acctacagca aaacagacag ttggaatcaa
     1141 agtcagagca aataagaaac catcttttgc tcccgttcct cctttaccca taaagtctac
     1201 accatctgca atagtaccaa atgaacctgt cagattagca aaatctaatg ctttaaggaa
     1261 gccttgacta tctttaaata ctcctgaaaa taatactatc aatacaaata aagataaata
     1321 ccctttccat gttaccttat tatctccagc tgttacatcc atataaaatc cccctttata
     1381 cctcaaattt atctaagttt aaaataactg tagaaattaa tttcaccctc tcaaacatag
     1441 aatctactac tgcatattct cttgatgtgt ggttccattc tcctcttaca ccaaatgaac
     1501 agattgtagg aacgtttgca atagttaaat aacttgcatc tgaactacct ccaagtctct
     1561 tagcatttaa ttctccaaat ccattttctc ttgacacttc atttacaaat gtatgaaatc
     1621 tcataacatc ttctgtagtt tcaaatggca tcatttcact tataaaatta acttctgtag
     1681 aagttccttc tatgtaagtt tctttacata tctctctaac ttgttcttta acattctcca
     1741 tttcttctac tttttcaaat ctaaggtcta cttttatact acaatcttct ggaatagaat
     1801 tttccactct tccaccctta atcacactta cacttactgt agttccaaca tctaaattag
     1861 tcaatttttg tatgcgtagt atcttattag ccatttcttc tatagcattt cttccacctt
     1921 caaaatcatt acctgcatgt gtttctacac cttttacatg tatattacat cctattcttc
     1981 cttttctacc tatacataaa ctattatcta ctaatccagt ttccatatta aaagcacaaa
     2041 gagcaccttt tgcttccctc aatataacat ctgcaccagt tgaatcttta tgacctatct
     2101 cttcatctcc agaaaatact atttttattg gtctttcttt gtatcctatt ttatttaaag
     2161 ctttaataac gtataaagag attattatac cccccttcat atctaaaaca cctgggccat
     2221 atgcttttcc ttctattatt ttaaatggat tctctccaaa tgtacctgtt tcaaagacag
     2281 tatccatgtg tcctgaaaaa attatcggct ttttatctat attagaacca agtgtaccta
     2341 tcaatgtatt accattttcc ccaacatcta ctagttcaca atccaaacct tctttttcaa
     2401 attcaagctt taatctctca gctaacttgt ttacactttc tttacaatgt gtataacttt
     2461 ctatattaac aatctctttc cataatgaaa ctatttcttc tctattctca tcaacaaact
     2521 tagaaatttc atttaaatat gtattcataa tcttctcctt tattttcaaa taaaatgtat
     2581 ttctttccta aaatctaagt ttattttagg aaatcttaaa agactagtcc ttaattacaa
     2641 aatagcacaa ttattcttgc cagtcaacat atttttcagt cttttttata ttttctgatt
     2701 tttctttcaa ttatcctttt gtaaaataaa ttgacgcgtt ttatctacta ttataaaatg
     2761 aggttataaa ttgattgatt agttacaata ctctcgaaat tttaaaggag gttttatgaa
     2821 atataaaatt ggttttttga caacatctta ttttatgtca gataagttta aacaagctct
     2881 taaagaatta aatgatactt gtgaaattac atttattgag cttaaagggg agaatccttt
     2941 taataattta cctaatatat actatgagca agtagataat tttgatggat ttgtatgcag
     3001 tggtatagtt ccatatagtg aacttataat aaatataaaa gacataaaag ttccattaaa
     3061 ttttttaaag cttgatgaaa gagattttta taaatactta ttcaaattat taaatagcca
     3121 aaaagatatt gactttacaa aatcatttat ggatttttta agagaggaca acaattatta
     3181 tgatatatat tcattaatag actctaataa atgtccatat acaattaaag attttaatat
     3241 ccctcaacct gtatatgact ttaaaaaatt aaatgataaa ttattggaaa tacatttaga
     3301 cttattgcga gataataaga ttaacctttc ttttactaga aattacatca ttagttgtga
     3361 acttaataag cttggatatg attgtattta catggttcca tctacagaat caatcctgaa
     3421 tacatttaaa tctctggtga atgaaataac attacaaaac ttagacaaaa ataaatctgc
     3481 aacttgtata gttacagtaa atagttcaga gtacataaat gaagatttga tatttaaaaa
     3541 tgatgagata caaaatcaaa tatataacac tatattaaat tctctatcaa gacatggatt
     3601 ctatggagtt caggtcaaaa aaaatgatat gaaaatagag atacacacta ctaaagaaat
     3661 attaaacaat atgaccaata actatacaga tttttttatt tcagaagatt taaaagaaat
     3721 taaatatagc ttaaatatag gttggggcat tggaaataca aatattcatg cagagcaaaa
     3781 tgctcgtcag gctaatggta aatctgcttc tttgaatgga aactgtactt ttatagtaaa
     3841 tgatactaat gatacaatag gccctttata ttcaaataaa aattctaata atattgaaga
     3901 tagctcaata gccaataaag ttgcttcttt tatacctcta tctaatacaa atgtatctaa
     3961 aatcatgtgt atgattaatg atagaaattc aaataatgtg tcagcagaaa tattagcaga
     4021 ctatatgaac ataactctta gaagtgctaa tagaatattg agtatcttat ataaagcagg
     4081 aattgcaact attgtaaata caaaactcga taatcaaaga ggaaggccaa aaaaaatata
     4141 taaagttgat ttcttatctt ttctaaataa gatgcagaaa ttaaattctt aatatttaat
     4201 tttttaataa aagcaatgta ttttctctaa ttttaaagaa tcaaaatgtt taaattttat
     4261 tagaatatac aactttttac tttaaaatac ctaaaccttt tatcagtgaa ataaaccata
     4321 atatatcgta gcttattaga aaaacaaaat aatttcatat acaaataata aaatagccac
     4381 tacaatccat aatttgcagt ggctactttt tacactatca cttattactt aatttttaaa
     4441 aattaattgt attttttaaa taatttatac acacctatta tcagtaaaac aataaataca
     4501 agtatatcta taaaaataat tattgtaaca tgtctggcaa tattgtaaat aaaaagtata
     4561 gagaatattt ctatagacaa cgctacaaaa cacataaaat tcgttaaata atctgcaata
     4621 gtatcactat aaaaagcatg tatttgtaac catctccata taaatggacg taaaaggatt
     4681 ctgaatacaa cacaaatgac agggtaggca taaataaatt ttttatctac ataattagct
     4741 acaccattat tccattggat aggaatttga ggtggcaact ttggaaatac tgttattgca
     4801 atcaacaaac aagcaatagt aataattccc caggtaattt tccctcccca tatatataag
     4861 aaacttacag agtcaagttt aaaaacaggt ttgtttttat tccagtattc tggcaactca
     4921 gttatgtatt gctctacttt caaattcaaa taacttagct catcagattc taacatttta
     4981 ttacacatag tttttgcatt ttctaagtct gatattttag tttcgagaac tttctcctgt
     5041 ttctctacaa cttcaactaa agatactttc tgtaatataa tattacggat atcatcaata
     5101 gtaattccta gtgtacgtaa atatgcaatt tttttgatgt cctctacatt ttcatctgaa
     5161 tattctctat agccattacg ctcattacga aggggtttaa ttaaattctc gttctcataa
     5221 aaacgaatat tagagcgtgt caatcctgtt ttttcttcaa cttctttaat tgtcatttta
     5281 aacactacct ctctattcta ttaatttaca actataatat agtcttaaat attttcactc
     5341 tataaaattg tatatttagt atatcttaaa atcaagttaa ttatctttta taaattttca
     5401 tacaataata tctcctttca ataatatctc tttttctata tttaaaacta tcttattttc
     5461 tataaaataa tgtataaaca aggtttacag tcaagtattt ttattatttt tttgatttca
     5521 taaaaaaaag catagcaaat catttgctat acttttttac taaattattt attctatatc
     5581 ttatatgaac ttattgttta actactacac aatactagct ttaatcatca tattttttgc
     5641 aaacatactg gcttcatata tttcttgttc atctggatga ctttgagatt cctcatggtt
     5701 atttaattgt tggcgaatat gttcgttgtc aggagaaagt tttaacatct ctttatattt
     5761 tgctttcaat ccttctgcta tcttaccttg acatatgaat gaacctaata tttcattatc
     5821 ttttgggata attgattcta tcttttctat tatactagct ccataacttc cttttttact
     5881 tgctcccatt gttccaaata gtgctatctt tttattgtga atattttcaa gcacttgttt
     5941 tgaattttta tcacaaattc ccttgtccac ccaatagcct aagaatacta tatcataatt
     6001 ttcatagtca ataccatact gaacctccca tgactaaagt cacagggttc ctgcttcata
     6061 gaattttgca tactaaaata tgtcttacta attctccaca ggctatcccc gtagttccta
     6121 cggttcttac ataagttatt tattttgaat tgttattagt cttaatcctt cttttaatat
     6181 atttatactt gcatttatat ctctatcgtg agtttcatta caactaggac aaatccattc
     6241 tcttatattt aaatccttaa cttcctcgtt tttataccca catttattgc atatttgtga
     6301 acttgcaaag aatttaccta cctttacaat ttgtctacca taccaattag ccttgtattc
     6361 taattgacga ataaattctg accatgatac atctgaaatt aagcgagata gtttacgatt
     6421 tctaatcata ttctttactt gtaaatcttc tatgcaaata atatcgttct cttttattaa
     6481 tttagtagat aatttttgca gaaaatcatt tctttgatta gctatatgtt cttgaagtct
     6541 tgcaactttt aatcttgctt tattcctatt taa
//
LOCUS       DAELSY010000190         6519 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.190, whole genome shotgun sequence.
ACCESSION   DAELSY010000190 DAELSY010000000
VERSION     DAELSY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6519
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.190"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1655)
                     /locus_tag="KPG09_003447"
     CDS             complement(<1..1655)
                     /locus_tag="KPG09_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0968009.1"
                     /translation="MKYLIEIAKKERLLVFIYLALGIGIELLNSFSANYYQSLIDRFN
                     NSTINFHIIFIYGTVLVTLCLLRYIDEYPGRRLEHSFFLDLKLKALEKMSKIDYLEYQ
                     MLGTGKLIQKIENGANAGRNIFFNFLLAVVRKIIPSILFSMVFIYRIDKKVMFTILIG
                     YFIVFIITNLLIKALYQIKEHILINEEKMNHFLVRGFVEMVVFRVNKRFEYEICQSKF
                     AKKEIIDSRVKMTLIHEAFFTIFSLIVTFIKMLIIVYSWKTKTISIGSIIALITLLDN
                     AYTPIAIFNVLYIQFKLDKAAYKRYTDILELNNDGQLLAGKEVNLMYGNIEFNEISFL
                     YNNKREIFNKLSLSIKSGEKVAIVGESGSGKSTLIKLLIGLLKPSEGTIFIDDYNLLE
                     MNLNSYYNYVTYVSQDSPIFDGTLRENIVFGKTIKSNDIIEVLEKVGLKNLYLKLEAG
                     LDTKLGEKGTTLSGGEKQRLALARLWFSDAKIIILDEATSALDNITEEKVMLNVMEFL
                     KDKTTIMIAHRFNSIKNVENIIVLKEGSIIGSGSFKKLLDGNL"
     gene            complement(2578..3159)
                     /locus_tag="KPG09_003448"
     CDS             complement(2578..3159)
                     /locus_tag="KPG09_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0968010.1"
                     /translation="MNNKKHWYDYLWIFSSIYLVLGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(3152..3382)
                     /locus_tag="KPG09_003449"
     CDS             complement(3152..3382)
                     /locus_tag="KPG09_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0968011.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            complement(4707..4982)
                     /locus_tag="KPG09_003450"
     CDS             complement(4707..4982)
                     /locus_tag="KPG09_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0968012.1"
                     /translation="MLELTKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            complement(5058..6359)
                     /locus_tag="KPG09_003451"
     CDS             complement(5058..6359)
                     /locus_tag="KPG09_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0968013.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
BASE COUNT         2230 a         1203 c          535 g         2551 t
ORIGIN      
        1 tataaattac catctaatag ttttttaaag ctaccactac ctattatact cccctcttta
       61 agaacaatta tattttcaac attcttaatt gagttaaatc tgtgagcaat cataatagtt
      121 gttttatctt tcaaaaattc cataacattt agcataactt tttcctctgt aatattgtct
      181 aaagctgatg tagcttcatc taatataatt atcttagcat ctgaaaacca aagccttgct
      241 aatgctagcc tctgcttttc tccacctgat aatgtagttc ctttttcacc taatttagta
      301 tctaaaccag cttctaattt taagtataaa ttttttaaac ctactttttc taagacttca
      361 ataatatcat tactttttat agtttttcca aatacaatat tctcacgtaa agttccatca
      421 aatataggtg aatcctgtga cacatatgta acataattat agtaactatt tagattcatc
      481 tcaagtagat tataatcatc tataaatata gttccttcgc tgggctttaa taatccaatt
      541 aatagtttaa ttaaagttga tttaccagaa ccactctctc caacaatagc tactttttct
      601 ccacttttaa tacttaaact aagtttatta aatatctctc gcttattatt atacaagaag
      661 ctaatctcat taaattcaat atttccatac attaaattaa cttctttacc tgctaacagt
      721 tgaccatcat tattaagttc caaaatatct gtatagcgtt tatatgctgc tttatctagt
      781 ttaaattgaa tatacaatac attaaaaata gctataggag tatatgcatt atctagtaaa
      841 gttatcaatg caattataga acctatactt attgttttag ttttccaact ataaactatt
      901 attaacattt ttatgaacgt cactattaat gaaaaaatcg tgaagaaagc ttcatgaatc
      961 agtgtcattt taaccctaga atcaataatt tcttttttcg caaattttga ctgacaaatt
     1021 tcatactcaa atcttttgtt tacccgaaat acaaccattt ctacaaatcc acgaactaaa
     1081 aaatgattca tcttctcttc attaattaat atatgctctt tgatttgata tagagctttt
     1141 attagtaaat tagtaataat aaaaactata aaatatccaa ttaatatggt aaacataact
     1201 tttttatcaa ttctataaat aaaaaccata ctaaataaaa ttgatggaat tatttttcta
     1261 acaacggcta ataaaaaatt aaaaaatata tttctgccag cattagctcc attttcaatt
     1321 ttttgaatta attttccagt gcctaacatc tgatattcta agtaatctat tttactcatt
     1381 ttttctaaag cctttaattt caaatctaaa aaaaaactat gttctagtct tctacctgga
     1441 tattcatcaa tatatcttaa aagacataaa gtaactaata cagttccata gataaaaatt
     1501 atgtgaaaat ttatagtact gttattaaat ctatctatta ggctctgata ataattagca
     1561 ctaaaactat ttaaaagttc tattcctata cctagtgcta aatatataaa aactaataac
     1621 ctctcttttt ttgctatttc tattaaatat ttcataactg tacacctatc cttatttatt
     1681 ttactttatt aacttaaaat aattttatag ataaaatgtt tctttatttg tttaaattta
     1741 gtgtgattat taatcatttc aagttaacaa aacctaaagt ttttagcagt acagttctat
     1801 ttgttagcaa attattattt tactcagtac attatgctaa catataatct aactgtactg
     1861 agtaaatatc tcgtttcatc atataaaatc tcctttttat agtttaatat atatagttaa
     1921 atttactata taatctgaat atttattttt ctatcacctt taccagaaat aaaatcaatt
     1981 atttattgta taagcaaaac ttcataatat tttcaacata taattattat acagaactcc
     2041 taagttaatt taaagttaga atttattttt ataagaaatg tcattgacct acctttaata
     2101 aaacagccac cattttttta tgtattaaca tataatatat agtaatgaaa acatattaat
     2161 taaaaaatat tgtataaatt agtttatctg taacaaatcc agtttatgtt tcaacaagca
     2221 cgtaagactt cttgaaattt aatctgcttc ctttaaatat ctattacttg ttttaagcat
     2281 aagtttacaa tcttctataa catttatcat ttcttcagat ttctctgacg tacttaaaat
     2341 acaagttgtt acattgtttt tctatttaat tagtaatgta tcatgtaaaa aagaagcata
     2401 aatgagttta ctttatacaa acttcattta tgctccactc atatacctat catagctaat
     2461 agaaactatt actcatcttg aatctatttc atatttagat atttatatct ttcttatttt
     2521 cttaaatcaa ctctagcttt aaacattaaa ccccttaaat cttcacaaaa tctcttccta
     2581 tacagactta ctctttgcct tacatatcat ctgagtcata gtacccatag ggcaataaac
     2641 acaccatgaa cgaggtttaa acaatatcat agtaatcaaa ccaagaacag ttgatgtaag
     2701 cataacacta taaaaaccaa atgcaaactg taccacccaa ggtgaaacta aagttccatg
     2761 atacgcccat tgccaaggca tttttatagt ccacaacaaa gtcactactt ctttaaaatt
     2821 tctacttcct tcaaacacta aataagttga aaaaagcata ttggcaaaca ttaccataaa
     2881 aaatgtcaaa aacccatatc taaaccactt acttctaata aatttaggaa tatctctatt
     2941 tcttgacagc ttcaatttat ttccaagcaa attaaacagt tgccctcttc cacaatactt
     3001 attacagtaa gctttatttc ccttaacaat agaaatagct agtggaacaa agaaacaaat
     3061 tagcccaatc caagcgaaaa taatatttac aaatcccaat accaaatata ttgaagaaaa
     3121 aatccataaa tagtcatacc aatgtttttt attattcatg acacttacct tcttcctctt
     3181 ttaatattgt tataatctca attacagatg ctggacatat cttttcacaa atcccacatc
     3241 caacacataa atctctatta atttttgcac taatgccaca tggaactgag atagcacttc
     3301 ttggacatgc ttttatacag ctaccacaag caacgcactc atttgctaaa atatttgcat
     3361 acttcttttt tttattagac ataataacct ccctatatta ttaatctata agaattatat
     3421 caagatactt actttttatc tgtgacttaa tcacataaaa cctcaatatt aaaaatcaca
     3481 ctctaaatac aaaggagttt caaataaaaa acgatatgaa gtcattaagt ttagcttcat
     3541 atcgcctttt cttttgtgta tctatataaa tatttattat ctaatattta atatatctat
     3601 aatattttct tatacagctc tataatttta cacctaatca tattctatta gtaacaggtt
     3661 aatacccatc taaaactata actttattca caatcttatt ctattagcca caagttaaac
     3721 tcatattgaa gtttatcttt agttacactc ttactctatt aatcacaagt taagcccata
     3781 ttgaagttta tctctattta cactcttact ctattaatca caagttaagc tcatattgaa
     3841 gtttatctct atttacactc ttactctatt aaccacaagt taaactcata ttgaagttta
     3901 tctctattta cactcttact ctattaatca caagttaagc tcatattgaa gtttatctct
     3961 atttacattc ttactctatt aaccacaagt taaacccata ttgaagttta tctctattta
     4021 cactcttact ctattaacca caagttaagc ccatattgaa gtttatctct atttacattc
     4081 ttactctatc aatcataggt taagctcata ttgaagttta tctttagttg caccttactc
     4141 tattaatcac aagttaaacc catattgaag tttatcttta gttacacctt actctattaa
     4201 ccacaagtta aacccacatt gaagtttatc tctatttaca ctcttactct attaatcaca
     4261 ggttaaacct attataatct taactttatt cacaatttta ttctattagc cacaggttaa
     4321 atctattata atcttaactt tattcacata ttacatcatt aatcgcatat caagatttta
     4381 taataagctt taactaaaca cacgatttta ccttattcgt atagtttaat ctattaaatt
     4441 attatataat cttaagcaaa catacaactc tacactatta attatagccc ctccttttaa
     4501 atactcataa tcttatacac aaccttacct tattaatcac acatttaact catatatatc
     4561 atcaatttta ttcacaaaat catgctgtta atcacactta tactcctcta taatcttaaa
     4621 cttatacaca aattagccac aattttattt caactataat aacttctatt tcagaagtat
     4681 atcacacttg tattctccaa aaatatctat ttttttctaa caatatgcct gacattaata
     4741 acactcattt caaacatcct cgctaattcc atataatttt tcccattaaa caatttaatc
     4801 ataagctcat ttctagcctc ttttaatgcc ccttccagtg ttttaatata aaaatatgtt
     4861 cccccacttt gttgacacaa ttcaaggtat tccaataaac tatacttatg taactccttt
     4921 ggaatactta cttctatctt gtgttcttca agtaattcta taattttcgt taattccaac
     4981 atattttcac ctcatatttt ataatctatt tgctatttcc tgacttttaa taagcttgtt
     5041 attactactt tattgaccta ataatttagt tcttgaaaat gtaacttatc cccaaagtaa
     5101 caaaaatgct tatgcctagt ctgtccatct ctacacttcg caactattaa atcaaccatc
     5161 ttaattcccc tgtattctga tgctctaact gcctcctcac tttctccaat atctctcact
     5221 gcttcttcat aatcactccc ctttaattga tgtatataaa ccacattgtt gctatcgtgg
     5281 tagatggctt tgctatccct cataggtcta tctccataag gtcttgaatc tttcatttca
     5341 tcatttaact gacttaattg gattacaggt atattaaaat ctaaagtcat atttttaagt
     5401 tctctcgaaa aagtagctac ctccctttct ctcttatcca tgcttttctg agatgtaagg
     5461 agttgcacat aatccactat taataaatct ggctttacct gccttatcct ctttctaatt
     5521 gcactaattg tatctatctt atcattaata taaattaagt tattttcgct taaatcacct
     5581 atagcattta caataagttt ccaatcattt tcatctattt ctttagattt catattcctt
     5641 gtacttacac cagttactct gcatatattt ctcataaata cttgctcact ggtcatttct
     5701 ctacttataa ataaagtttt tttaccttgc ttaaatgaat ttaacattat ttgaagtgcc
     5761 aaagcagttt tcccaacccc cgatttagct gctattgtgg ttagctctcc tttaaaaaga
     5821 cctcctatag tagtatcaag taattttata ccaaatttaa atccaatatc ttttttgttt
     5881 tctaaaaaat caagcacctt ccctgctata ctatttacat catcattttc ataagtatcc
     5941 ttgtccaaaa tctcctttat attactttca aatttgtaaa tacaagtatc gatattttct
     6001 ccaaggttta ggctgtgtaa aaggttttgg cagttttgat ttatgcttct tctagctaaa
     6061 ttttctttta aaattttaat atgaccatca agaccaaaat tctgacccca agtaattaga
     6121 ttagtcacat cactcgtttt tattgctaga gattttctat ctatctttga tttcacagtg
     6181 gccacatcaa tcactatgtt ttctttttta agttctttca taattttaaa tatctcaaga
     6241 catatatcat tcataaacat gttttcattt aattcatcta gcttatagct taatttattg
     6301 tctaaaagaa ttgtgcctaa aatagacttc tctatttcaa catcttttga tttacccata
     6361 taaattaatc tctctttcct tataagtttt tatattttga tttttaaata cattatcaat
     6421 tataaataca ttatttgtta tgactcacta tttgttatga ctatattatt cgtcatgata
     6481 tactacaaat tattaatata ctatttattc tgatacata
//
LOCUS       DAELSY010000191         6470 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.191, whole genome shotgun sequence.
ACCESSION   DAELSY010000191 DAELSY010000000
VERSION     DAELSY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.191"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            319..1263
                     /locus_tag="KPG09_003452"
     CDS             319..1263
                     /locus_tag="KPG09_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBG0968014.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1285..1911
                     /locus_tag="KPG09_003453"
     CDS             1285..1911
                     /locus_tag="KPG09_003453"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBG0968015.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1945..2994
                     /gene="hisC"
                     /locus_tag="KPG09_003454"
     CDS             1945..2994
                     /gene="hisC"
                     /locus_tag="KPG09_003454"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG0968016.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2997..3575
                     /gene="hisB"
                     /locus_tag="KPG09_003455"
     CDS             2997..3575
                     /gene="hisB"
                     /locus_tag="KPG09_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBG0968017.1"
                     /translation="MRIWKVERNTFETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3577..4182
                     /gene="hisH"
                     /locus_tag="KPG09_003456"
     CDS             3577..4182
                     /gene="hisH"
                     /locus_tag="KPG09_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBG0968018.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDITLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELVAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4179..4901
                     /gene="hisA"
                     /locus_tag="KPG09_003457"
     CDS             4179..4901
                     /gene="hisA"
                     /locus_tag="KPG09_003457"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBG0968019.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGINTKIIEDIANNIDIPIQVGGGVRDKGKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSDVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4883..5641
                     /gene="hisF"
                     /locus_tag="KPG09_003458"
     CDS             4883..5641
                     /gene="hisF"
                     /locus_tag="KPG09_003458"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBG0968020.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKNMGVEVRL"
     gene            5669..6337
                     /locus_tag="KPG09_003459"
     CDS             5669..6337
                     /locus_tag="KPG09_003459"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBG0968021.1"
                     /translation="MDNKCNNVYSDEVEEFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQKLWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMEPEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2644 a          542 c         1267 g         2017 t
ORIGIN      
        1 attattatta atagtaataa aaaactgtaa atttagaata ttaataaaaa ttatttggga
       61 ggaaattatc atggtgagat taaatatagt aaaattaaat acaaaattaa atcatcacca
      121 tcatcataga ttaaagggat tgtaatatga aattaattca tagacacaag ccctgttttt
      181 agtgtaattg gtcttgtatc tatgatggga agtaatttta ggagaaaatt atagccatat
      241 aggtataaag cctatatggc ttttttattg ttttaaattt gcaaatataa gagtaaattt
      301 aatgaagagg gtgagaatgt gaaatttttg aggattgaag atgaagttat ttattctaaa
      361 aaaagatata aattaaaaag ggaaatagat aaactttttt tggatgaaga atattttcaa
      421 atagaaccac aattatttga agaatatgat gaatttacaa ctataaataa taaaattcca
      481 gaagaatcta tggtaaaggt tgtaaatgga aaagtaatgg tacttagagc tgatataact
      541 acaagtataa taaaaagcct tgtaccaaga tgggaagatg gtttaaagct taagcttttt
      601 tataattcat caatatataa aaataaaaat acagttggaa taaaagagat aagacaaata
      661 ggttgcgagt atctaggaga agcctctgta gaagcagata gagaagtagt gaaattagct
      721 cttaagatac ttgaaaaata caataataat tttattttag aggttgggag tagcaagtat
      781 attcatggtt tgttagaaga attaaattta aataagaatt gtgaaaatca aattaaaaat
      841 ttactatata cgaagaacac tcatgaatta aaagtatata ttgaagaact gaaaattaag
      901 cgtgaagtaa aagagctatt atcaaatatt ttaagcttgc aagggaattt atacaacgtt
      961 attgaaaaat caaacaagtt ctattgtaat aataagatga agcaagctct tgaagaatta
     1021 aagcaagtaa ataatttaat tgaggaatgt aattttttag ataaggctag atttgattta
     1081 tcaatgatta ctatgcttga ctattatgaa ggaattatgt ttagaggata ctacccaaac
     1141 tcatataaag aaattcttag tggtggaaga tatgattctt taactaaaga gtatggaaaa
     1201 gaaattccag ccattggatt tacactaagt gttgatgagt taatgaagta tgtatataag
     1261 taaagtttag aaggagggat tgctttggat tatttgacta tagcactagc aaaaggacga
     1321 attgaagggg aatcatttaa aaaatttaaa aaaatgggtc taggagatag tatagacaca
     1381 gatacgagaa aactaatttt taaagatgaa gaaaataaaa ttatatatat acatgtaaag
     1441 ccatcagatg tagttacata tgttgaaaaa ggagtcgctg accttggtat agcaggtaaa
     1501 gatactattt tagagaatga gacagatgta tatgaaatat atgatttagg ttttggaaaa
     1561 tgtaaatttg ctgtagcagg attaaaggga gatagcatat atagagaaga tgaatatcta
     1621 aaagtggcaa caaagtatcc caatatagca aaaaagtatt ttaaagagaa aggtcaaaaa
     1681 attgaaataa taaaattaaa tggttctgta gaactagctc caatagtagg gttgtcagat
     1741 gtaattgtag atatagttga gactggcaat acacttaaag caaatggtct tgaaatatta
     1801 gaagacatat gtaatataag tgctagaatt atatcaaata gagctagcta tagatttaaa
     1861 tatgaacaaa ttcagaatat aattcgttta tttgaagaac ttgataatta aaatataatt
     1921 gaactttata aggagggatt taagttgaga gaaaaagaaa gtataagaga gttaagggga
     1981 tatgaaccaa atcacgtaaa ctgtaaagta aaacttgatg caaatgaagg aagtaagagg
     2041 ttgtttaaat accttattaa agaaatttca gatagtgata tagatttaaa tttatatcct
     2101 gaagattctt atagtgattt gaaagaatct ataatagatt acataaatat ttctggagta
     2161 aataagaaaa atcttctagt aggaaacggt tcaagtgaaa taattgattt aataatacat
     2221 acttttgtag ataaagatga agtaatattg agtttctctc caagttttag tatgtattct
     2281 atatatagtc agataaatgg gtctaaattt ataggtgttg aaagtgatga aaatttagtt
     2341 ataaatatag ataatgtgat tgaaaaagta aaggagaata atcctaaaat agtaattgta
     2401 tgtaatccta ataatccaac tggaacaata ttaaagaggg aagagataat aaagctttta
     2461 gattcaacta atagtctagt ggttttagat gaagcatata tggattttgg agaagaaagc
     2521 atgttaagtg atgtttttaa gtatgataat ctaatagttc ttagaacttt atcaaaggca
     2581 tttggattgg ctggaataag aacaggatat atgttatcta atagtagttt aataaattct
     2641 gttgagaaag taagacctcc atataattta aattcattat cagactttat tgctacaaga
     2701 gcacttagaa ataaagatgt ggtaaaggct tatataaagg aagtaaaaga ggaaagagaa
     2761 gttttatatg aagaaatgat tggtatggga attaaagcat acaaatcaca agcaaatttt
     2821 atattatttt attctgaaat cgaaaattta tcacaaaaac ttattgatag aggagtttta
     2881 ataagaaaat ttggtggaaa gttagaaaat tattacagag ttacaattgg tgataaagaa
     2941 gaaaattcta tgtttgttgg agctataaga gatattttga aaaaggagaa ataggtatga
     3001 gaatttggaa ggtagaaaga aatacttttg aaacacagat tttagtagaa ttaaatattg
     3061 atggttctgg taaagctgaa atagatactg gaataggatt tttagaccat atgcttacct
     3121 taatgtcatt tcatggcaag ttcgatttaa aagtgatttg taagggtgat acatatgtag
     3181 atgaccacca tagtgtagaa gatataggta tagctatagg tgaagcattt aaaaatgcac
     3241 taggtgacaa aaaaggaata cgaagatatt ctaatatcta tatacctatg gatgaatctc
     3301 tatcaatggt agcaatagat ataagtaata gaccttatct tgtatttaat gctaaatttg
     3361 atacccaaat gattggtagt atgagtactc aatgctttaa agaatttttt agagcttttg
     3421 taaatgaatc gagagtaaca ttacatatca atctcttata tggggaaaat gaccatcata
     3481 agatagaaag tatttttaaa gcatttgcaa gagctttaaa agagggtagt gaaatagttt
     3541 ctaatgagat agcatcttca aagggggttt tgtagaatga atataatagt agactatggt
     3601 cttggaaata tagattctgt gtctaggggt tttagaaaag ctggaataga aactaaaatt
     3661 tctagtgata tagatgaaat aaaacaggca gattcactta tacttccagg tgttggagct
     3721 tttagagatt ctataagtgc cttagataaa ctaggattga taccaataat aaaggagcat
     3781 gtttctaagg gtaaatttat gataggtata tgtctaggaa tgcaattact ttatgaaaaa
     3841 agttatgagt atggagaata tgaaggatta ggacttataa aaggaagtat tgataaactg
     3901 gatataactt taaaagtgcc acatatggga tggaataatc taaaatttaa taaggcaaat
     3961 gatgatatac ttaaatatat aaacgaagat gattatgtat acttcgttca ctcatattat
     4021 gcaaattcat caaatgaaga actagttgca ttttcagaat atgaaaagaa aatccctgct
     4081 attgttagaa aaggcaatgt atatggtata caatttcatc ctgaaaaaag tggagaagta
     4141 gggttaaata tattaagagc atatggggag atgataaaat gataatattt ccagcaatag
     4201 atataaaaga taataagtgt gtaagactca cacaaggaga atttgacaaa gtaaatgtat
     4261 attatgataa tccattagaa gtagcatata aatggaaaaa tgaaggagca gaatatatac
     4321 atatagtaga tttaaatggt gctagaagtg aatttggtat aaatacaaaa ataattgagg
     4381 atatagcaaa caatatagat atacctattc aagttggtgg tggagttaga gataagggaa
     4441 aagtcaaaag tttaataaat gctggtgtta ctagagtaat attaggtagt atagcgattg
     4501 aaaatttgaa tttggttgaa gaattggtca atgaatataa agagaaaatt gtagtttcta
     4561 tagacgctaa agatggaaaa gtagcagtta ggggatggga agttgtaagc gatgtagact
     4621 cattaacatt atgtaaacaa cttgaaaaga taggtgtaca aactattgtt tatacagata
     4681 tatcaaaaga tgggatgttg caagggccaa attttgacat atatgaaaga atagcaaagg
     4741 aaacgtcatt aaatgttatt gcttctggag gcgtaacttc tatagaagac gtaaaaagac
     4801 taaaagctat gaacttatat ggtgcaataa taggtaaagc gctttatgat aaaaagatag
     4861 actttaagga ggcacaacaa ttatgcttac taggagaata ataccttgtc tagatgtgag
     4921 aaatggaaga gttgtaaagg gaaaaaaatt taaagatata gtagatgttg atagtccaga
     4981 agtacttgga aaattttata gtgattgtgg tgcagatgaa cttgtattct atgatataac
     5041 tgcatcaaat gaagagagaa agacatcttt agaatttgtg acaaaagttg cagaaaatat
     5101 aaatatacca ttttgtgttg gtggaggagt aaataaatta gaagatttca ctgatatttt
     5161 aagaaaaggt gctgataagg tttctataaa ttcatcagca gtcaaaaatc ctgaattaat
     5221 aagggaagct tcattaaaat ttggagcaca atgtgttgtt ttatcgatag atgcaaagaa
     5281 aaatgaagag ggttcatgga gcgtatacgt aaaaggtgga agagaaaaaa ctaatctgga
     5341 tgcaattgaa tgggctgtta aaggtgtaga acttggagca ggagaaattg ttgtaaatag
     5401 tatggacgaa gatggaatga aaaatggata tgatatagaa ttattatcaa agataacttc
     5461 tttagtaaat gtacctgtca tagcatctgg aggcgctgga aaaaaggaag atttttatga
     5521 ggcagttaat aaatctaatg tagatggaat tttagcagca tctgtttttc attttggcga
     5581 gataaaaata aacgatttaa agaaatattt aaaaaacatg ggtgtagaag taagattata
     5641 agttaaatac taaaaggaga ttagaaatat ggataataag tgtaacaatg tatacagtga
     5701 tgaagtagag gaatttataa gaagtataaa gtttgatgat aaagggttag tccctgttgt
     5761 tgttcaagag gtagttagta aagatgtact gatgttagca tatatgaata aagatgctat
     5821 aaagaaaacc ttaaaagata aagtagcttg ctattttagt agaagtagac aaaaactttg
     5881 ggttaaaggt gagacatctg gtaatactca aaaagtagtt aagatgtctt atgattgtga
     5941 tgtagatact atactacttt ttgttgaaca aactggtgta gcttgtcata caggcaatta
     6001 ttcttgtttt tacagagact tgtttgatga tagttcaaaa atggagcctg aagtacaaac
     6061 aaatatattg aaagaattat atgatttaat caatgaaaga aaaaataatc cagttgaagg
     6121 ttcttatact aattatcttt ttgaaaaagg aatagataag attcttaaaa aagtaggtga
     6181 agagagtagt gaggtaatta tagcatcaaa aaataccgat aaaagtgaac ttatttacga
     6241 aattagcgat ttagtgtatc atacattggt tttaatgata gaaaaaggtg ttgaaattga
     6301 tgaaataaaa aaagaattac ttaaaagaag aaaatagaat aggatagatt aatggtaata
     6361 agaaaatata ccgcttttaa taaaaagtgg tatatttttt attttatatt ttcgatttat
     6421 agaataatat ttaaattact tgataaaatt aactatatat ataatttatt
//
LOCUS       DAELSY010000192         6173 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.193, whole genome shotgun sequence.
ACCESSION   DAELSY010000192 DAELSY010000000
VERSION     DAELSY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.193"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(175..390)
                     /locus_tag="KPG09_003460"
     CDS             complement(175..390)
                     /locus_tag="KPG09_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968022.1"
                     /translation="MHINQTGFISNGLGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFELIATLVREGSSWAFNFIVQP"
     gene            complement(573..917)
                     /locus_tag="KPG09_003461"
     CDS             complement(573..917)
                     /locus_tag="KPG09_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968023.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
     gene            1044..1427
                     /locus_tag="KPG09_003462"
     CDS             1044..1427
                     /locus_tag="KPG09_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968024.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
     gene            complement(2636..2887)
                     /locus_tag="KPG09_003463"
     CDS             complement(2636..2887)
                     /locus_tag="KPG09_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968025.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            complement(3133..3288)
                     /locus_tag="KPG09_003464"
     CDS             complement(3133..3288)
                     /locus_tag="KPG09_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBG0968026.1"
                     /translation="MALDVKIEVKIIKDVVILNIIHWKKETSLLTVKIARGQGDLSYV
                     KYFTNES"
     repeat_region   4505..5458
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4505..4533
                     /rpt_unit_seq="ctttacataccacttagttaatataaaac"
BASE COUNT         2204 a          867 c          626 g         2476 t
ORIGIN      
        1 atacattttt tataatttgt taaaataaaa ttcatatatt aagaaatatt aatagtaaat
       61 aagcaattgt tactataacg taggaattat ctttaaaaac acaaattaat ttacattctc
      121 taattttaaa gtatatgtat atctttgatt ttaataaaat tcacttaaaa tattttaagg
      181 ttgaacaatg aaattgaatg cccaagaact tccttctctt actaatgttg caattaattc
      241 aaacccacaa ttgtattgaa ctgaatctct acctactggt ttccaactag aaggtgcatt
      301 atatgagaaa gaatttgatg ttggctcttt cttttgttgc tgcttaattc ctagcccatt
      361 agaaataaat ccagtttgat ttatatgcac ccttctattt gttacacgaa tctggccgtc
      421 tgcaatagac cagtttccag aaactttact aacggaaata tattgaaatg ctccatctcc
      481 ttttgatatt gtatatgtaa ctgtaccaga tgcttttaca ccaccagttt tatcccatcc
      541 atcttctgat atacttgctt ttctaagagg ctttattgta ttagtatcaa aaaaactgtc
      601 ttagaatata tctttgatgt taaattttta gattgtttaa cttctaataa ttgagttgtt
      661 gatatagaat caacgtatat ggactctcct gttcttgcat ttttgattat acctttttca
      721 ttggaacttt taacatcagt aattccattt ttagccatat cgtataattt gtccatgtct
      781 gttacttgtt tctctgaata cattactttc atagatgaat cttctacctt actagcactt
      841 gcatttgttg gtaatagtat tgtcattatg agtaaagata aaatagttgt tacagatttt
      901 tttaacctat aattcatatt aatccttact ttttatttgt tataacagac attctattgt
      961 aatagaatat aaatccacaa tatttttaaa attagtattg aataactatt ctcatgttat
     1021 aattattcaa aggagggatt gatatgagga aaaaattttt tataggtatt cttatagttt
     1081 taataatctc tgtgatagct atacttaata taaaaaatga taaaaatgtc tttattggag
     1141 aatcagaaaa ttggaaagct aaatttgtaa ctactgaagg acactcaaaa ttagaaataa
     1201 aatataaaaa tagtaaagat gtagttgaac aaaaatttaa ttattcctat caatttatag
     1261 atggtgaaga tgcagttgta gttgtaaaaa atgaaatttt agaagcaggt caagaaaaag
     1321 caataaaaat acaaagacca ggtctttatt caaatagaga atctagttac aagctaatta
     1381 tatcttggaa tggaaatgag gaaactataa acttgaaaaa gaaataacca tattatcaat
     1441 ttttaaaaaa ttattattca aattcttata aaaagagtgt ttagaaaaat gaaacactct
     1501 tttttgtata agtttttgaa aactataaat gaaatatatc aaaatcgttc gaaaaattac
     1561 tgaaataaac tcttaaatat attataaatt aaggtatatt atacatgaat ttattgggag
     1621 tttatgagtt ttaaaattgg aaaccaaaca ataagggcta aaaatacttt aaggaaaata
     1681 tataaagttc tataaattat caaaaatatt tcgcaaaaat tgataattat tacaaataaa
     1741 ttgataatac taataatatt agaaatatcg aaattgaaca aactaggatt aaagctcaat
     1801 tagaaaaatc cactaatcct gagaatgctt tgttattctc atttattact gttgtttttt
     1861 cattatttgc taatttattt ttcagttcat taaatgttaa atataactct ataaatttaa
     1921 ttttacagtt aatatgtatt atagttctta tatttatata tattaataca tttttatatc
     1981 gcctcttcaa tttgagcccc atcattatat aaaattaacc tacttgatat cttaatttaa
     2041 tatatgtaca ctattcatat cttaatagtc aatattattc atttttaata gctcttttta
     2101 gatgtcagaa ttattatatt ttgaaattta ttaaattttt gcattaaaaa aactagaaat
     2161 taatttaagt cttgaattac ttataacttt ctacgtatct acctttatat tcttactctg
     2221 attttatatc taatattaac tcttcatcaa agctattaaa tatacctcta attgagtcaa
     2281 ttatttcact tacttcacct ttaattttta tattaacttc aaccatttca aatctcatct
     2341 ttttatctta taatttctat aagttttttt atttattgtg ttgcttactt tgaccagcac
     2401 ttttttattt aatagtctaa atgatatttt ttaccagttt taatatcctt aaatactata
     2461 tttactaaaa acttataatt tcttttaata cattcttatt actaataaca atactcaaca
     2521 attttatcgc cttttttcaa atatgattta acttttttat tcctaattat ttaaaaatag
     2581 ttgtccaatt ttcttctatg agaaagtgct aagtattagc aacttctcta ttctcttaac
     2641 tttctactgt ctcaactttc ttcatagcta tattaatagc tacttcttta gctacttcat
     2701 tgaactcttc ccatcttctc ctattttctt catcagatat acgtggatat actatataaa
     2761 cttctgtgtt ttgattagct ataatttttt catcatattc tatatttttt tctttatcat
     2821 atttgtatgg tttaaattgt tgtagtattt tcataacatc accccttgtt ataaaatatg
     2881 aactcataaa cttgtcttat acttatttaa ttgttacaat ttttacttaa aatcacttta
     2941 tattctatat tttaaagcta aatcctttac aaaagtaaca tattcttcta taagtttctt
     3001 atcctcttat atagtatcta gtttgtttaa cttatctctt ttaaacttac atacgccttc
     3061 ttctgccatt cgtcttttat taattgttaa cattgtttca agagacacac caaaactctt
     3121 gttaagaagc tcttagctct cattagtaaa atattttaca taacttaaat caccttgacc
     3181 tctagcaatt ttaactgtta atagacttgt ttcttttttc caatgtatta tatttaatat
     3241 aacaacatct tttattattt taacttctat ttttacatct aatgccatat tattgcttta
     3301 ttaatttgta tccttaaatc ttaaattttc tattaataca tcttctatac agcttagttt
     3361 ctctcgctta atcttgaaat atattttctc taaattgtca aattgttccc aagttttatc
     3421 aatatctaat attttgcagt gtctatttgc acatctttcg atccaaaggt ataattgact
     3481 agaatgtttt gaaatcaact gactattagt aggttagttc ttaaattctt tcaaatactc
     3541 gaattgtaat aaaacataat ttacattctc tttatttcta ttgaagttag tttatatatt
     3601 gtttacatca ttttcatata tatctgctag ttgctatgca tttaaaattc tcttattatt
     3661 tctttatatt acttgtaaat tattcatcct catcatcctc ttaattaaat ttctatctta
     3721 gaaatttaat ttgtaaaaaa attttctata tcataatcaa gtatcttaga tagtttatag
     3781 gtaacatatc aactctaatt gaatattacc ccttcctgta cttaaaataa gttgatgcat
     3841 ttttaaaacc taactctttt gcaacacttt gtaagtataa attttatgta tgtcttcttt
     3901 tttataaaat catagtgtat ctttttcata tattcacctt aattgctata tttcaataac
     3961 aatatttcca aaacagaaat ttttcgaggt aattatgtat agatgtaaat caaagaatta
     4021 aatatactag ggaaaatatg aatagttctc aaaaagaatt gcttgaaaaa acaaatataa
     4081 atacaagtgt tatgaataga atctggtgaa agaccaacaa gagacgatgg gttaataata
     4141 tttgctaaaa tttttggttt atatactgat tatgttcttg gtttatcaga tacagaaaaa
     4201 ctaaatattg gcaattaaaa ctgaattgat tgtattatat gtgtgatgaa tataaatata
     4261 gaattttttg agaagcttga aatagcttag aaaaaataag agattcactc aagaataagt
     4321 tactaaagat taaaaaataa caaaagtatc tactaaagga tatgaaaatg gtttaagaga
     4381 gttaagtctg gaaatattta tgtgtattct ttaaataaaa atttattttt taaatgataa
     4441 ttaaattttt actgatatgg ctatgctttt aaaagtcata accttatttt gatattagat
     4501 ctgtctttac ataccactta gttaatataa aactaaatat agcttgtaac tcctttgatg
     4561 cacttaaaag ctttacatac cacttagtta atataaaacg aatttagcta tagaagcatc
     4621 aaattgtggc acagcacttt acataccact tagttaatat aaaaccttta aagacaaaac
     4681 gtcgtataat taaatcatag caactttaca taccacttag ttaatataaa accttataat
     4741 tctttaagta ctagactgag caatttaatc tttacatacc acttagttaa tataaaacca
     4801 tcccattcag caacaacagt cgctaaatct tctgccttta cataccactt agttaatata
     4861 aaacttttag tatatttttt aggtctgccc tctttaaaac tctttacata ccacttagtt
     4921 aatataaaac acgaggttat agttaatttt gtcgtgggag tttataactt tacataccac
     4981 ttagttaata taaaacaatt tatatatata tatatattaa agtcagaccc ctttacatac
     5041 cacttagtta atataaaact tgttgttcag cattagcaat catatcatct attcctttct
     5101 ttacatacca cttagttaat ataaaacctg taccatcaga gacagtcatt aaaagattta
     5161 taaactttac ataccactta gttaatataa aacttataaa ttgtctctgt acccatttta
     5221 ttcaatgtcg tactttacat accacttagt taatataaaa ccatgaaaaa atgggtaatt
     5281 caaactcttt tattttcttt acataccact tagttaatat aaaacgttgg agaaaataat
     5341 ccttcttatg atttgccaag cggctttaca taccacttag ttaatataaa accagcggat
     5401 gaattattta tgttaattgg agatggagac tttacatacc acttagttaa tataaaaccc
     5461 caaaataaat tgagtatttc caacacttac atctatacaa ttctctcaaa tttgcagtga
     5521 accatgagta gtgcaaatga taacatttat cacataccct caatgcctta tatttcaact
     5581 gttaggccct attttatcaa aaatatcgct cactgcaaaa ctatttataa ttttataata
     5641 ttatgaatat attattttta aatatctgta ccaatttgta gtataataaa atcaagaaaa
     5701 taatttactc ctataaagag tgattatttt taagtgaaaa tattattttt ttaaatcatc
     5761 cttattggcg tttgggagat ttttatttta tcataaatat aaactgatat aacaccagct
     5821 gacatgcttg ctgctttcga ttattattct tgctacaatt attataacat ataaaactta
     5881 cttattttta ccaaatattt tatacaaaca ataaatatta acttatgaaa tatctactca
     5941 tttacagata tttcataagt taatagctct ataataaaat ttttatcatt gtttataaaa
     6001 aattttactt tgcatttttt tataaacaac agttttattt ataatgtttt gtggtataat
     6061 aaaagcaaga agaactacaa tctattttgc gttagagtgg agttcatcat aaacagagtt
     6121 tattttttgg atttaaatat aaatttaaat tcaactcgta agtcactctt tgt
//
LOCUS       DAELSY010000193         6139 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.194, whole genome shotgun sequence.
ACCESSION   DAELSY010000193 DAELSY010000000
VERSION     DAELSY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6139
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.194"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            129..1493
                     /locus_tag="KPG09_003465"
     CDS             129..1493
                     /locus_tag="KPG09_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBG0968027.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSNFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVENTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            1632..2798
                     /locus_tag="KPG09_003466"
     CDS             1632..2798
                     /locus_tag="KPG09_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG0968028.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            2915..3697
                     /locus_tag="KPG09_003467"
     CDS             2915..3697
                     /locus_tag="KPG09_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBG0968029.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYAESLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3816..4469)
                     /locus_tag="KPG09_003468"
     CDS             complement(3816..4469)
                     /locus_tag="KPG09_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0968030.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4493..5857)
                     /locus_tag="KPG09_003469"
     CDS             complement(4493..5857)
                     /locus_tag="KPG09_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG0968031.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         2318 a          762 c          927 g         2132 t
ORIGIN      
        1 tttttttatt ggaagattta tgttatattg agatgaattt attatgatat aaaaaataat
       61 gttaggataa atttaaatct ttttctaaaa atgtattaat ataattctta aagaaaggat
      121 ttttaacaat ggatataaaa gagttagtta aaatgcaaag aaaatattat aatacaggta
      181 aaacaagaga tatatcgttt cgtatagaac agttgaagaa acttaagtta gttgtatcac
      241 aaaatgaaga aaaaatactt ttagcactaa agaaagattt aaataaatcg aattttgaag
      301 gttttatgac agaggtaggc atgttttata gtgaattaaa ctttgctata aagaatataa
      361 gaaaatggag caaaataaaa agagttaaat caagtatggt taattttcca tctatatcca
      421 aagttgttcc acaaccttat ggtgttactc tcataatgtc accttggaat tatccctttc
      481 aattagcact tataccatta gtttggtcat tagcagcagg gaattgtgtt atattaaaac
      541 cttctgaata ttctgtgtca acatctagtg tagttaaaga catagttgaa aatacttttt
      601 caaaagaata tgttgcagtt gttcaaggaa gtcaagaaga aagtgaaaag ttactattgg
      661 aaagatttga ttatatattt tttacaggaa gtactaatgt tggtaaaata atcatgaaat
      721 cagcatcaga gcatttaact cctattactt tagaacttgg aggaaaaagt ccttgtatca
      781 ttttaaaaga tgctaatata gaccttactg ccaaaagact tacttgggga aaactaataa
      841 atgcaggaca aacctgcgtg gctccagatt atgttttagt gcatgaagat agaaaaaatg
      901 agttaataga gaaaataaaa tattatacta ataagtattt tggagataat ccatgtaata
      961 atgagcaatt tcctaaaatc ataaatcaga gacattttaa tcggatatta tctcttattg
     1021 ataaagataa gatagtctat ggtggaaatt ataataagga gacattaaag atagagccaa
     1081 ctatagtaga caatgttaat tgggatgata atataatgaa ggaagaaata tttgggccta
     1141 tatttcccat tttaacatat aaagatttag atgaagtaat acaaaaaatt atacaaatgc
     1201 caaatccatt ggcactttat atttttacaa aaaataagta cctagaaaat aaacttttag
     1261 aaatgattcc agcaggcgga tgttgtatca atgatacagt tactcatata gctacgaatt
     1321 acttaccttt tggtggtatt ggtgaaagtg gtatgggctc atatcatgga aaagctggat
     1381 tcgatacatt tactcactat aagagtgtgt taaaaaaatt aaatttggat gtccctatta
     1441 gatatgctcc atacgataat aaattaatta aggttcttaa aaagataatg taggttatat
     1501 tgtagaataa taaatataaa taaaattgag tatttgtatt ctgaatatat tttattttta
     1561 atttggtata tttgttataa aagtggtgat tttgtgataa aatttaatta tatagaaata
     1621 aggaggaaaa aatgaaaatt aattggaata caaaatacac aacaatagca atttatacat
     1681 ttataatagc tgctagtagt attatatttt atctagtatc atcacaaatt gatgtatttt
     1741 ctaataattt agatgctata tttacaacat tacaaccttt tataatagga tttgctattg
     1801 catatcttct taattttatt ttaaagtttt atgaagacag aatttttata aaaagtgaga
     1861 aattaaaaaa actaaaacaa agttcaaaaa gaggcttggg tcttttatta acatatgcaa
     1921 cagctacatt gatactttac ttatttatgc atttcgtatt gccacaagta attgagagta
     1981 ttgttggact agcaaatgat atacctatgt atgtaaacaa tgcaactaag cttattgata
     2041 aattaatgac tgatttaaat cttgatgagc aatactttaa tttggcagtt gataagtgga
     2101 atgagtttgt tacatatatt ataaagtttg taacagattt aatcccaata ttaggaagta
     2161 tgttaaagaa tgtagcctct agtatatgga atattgtact aggattaatt gtttcagtat
     2221 acttattaat tgataaagaa aaattctatg gattaagcaa aaaaataact tatgcagtat
     2281 ttacagaaaa acaagctgcc agaattttag aattaactca tagaagtaac tatacatttg
     2341 gaagattttt aggtggcaaa atattagatt cattcattat aggtattcta acatttgtaa
     2401 tacttacatt agttaaaatg ccatatacat tattaatatc agttataatt ggtattacaa
     2461 atattatacc tttctttgga cctttatttg gagctatacc atctactcta atagtgatgt
     2521 ttgtatctcc tataaaagct ttctggttat tgttgattat attaataata caacagatag
     2581 atggaaatat tataggacct aaaatacttg gagattcaat tggtatatct gcattttgga
     2641 ttttgttctc cttattggtt gcaggaaaat tattaggttt tattggaatg gttataggag
     2701 ttccaatgtt tgcagtaatt tattctataa ttaaggatat tgttgaaagt aaattggaca
     2761 aaaagggatt accgacagat acttccgatt acatgtagct aaaaaatatt atgtataatt
     2821 aaaaatatat aaattaagca atatttaaag cactgagaaa gttaaactct tggtgctttt
     2881 tagatataaa attattatag aagaggtgtt ttttgtggca tttatagaat gtaaatttaa
     2941 atctaaatat actggaggag aaactgatat aattgtaatt ttgcctgtag caaatggaaa
     3001 agaacttttt agtggtaaag agatatatga ttttgaaggt aaatttaaaa cattatactt
     3061 atttcatggt ttagaagatg accaaagctc atggattaga tatactaatg tagagcatta
     3121 tgctaaaaat aaaaatatag ccctagtaat accaagagta gaaactagtt tttatacaga
     3181 tatggcgtat ggacaccaat attttacttt tgtaagcgag gaattaccta aatttgttag
     3241 agctatattt cctttatcag ataaaaggga agataacttt gtagctggta tgtctatggg
     3301 gggatatgga gctttaaaat tagcactttc aaaaccaaaa gagttttcgg cagttgcaag
     3361 tttttctggt tcgcctgata tgattcatga gttagaaaat caacaaatag aaaccaatgc
     3421 agacatttta tttcacgatt ttggtagacc agaagacgct agaaatagtg agaatgattt
     3481 actttacata cttaagaacc ttaaagaaag aaatgaagat attcctaaaa tttatcaatt
     3541 ttgtggtgat agagattttt tatataaaaa taatcagatg tttaaaaaat atgcagagtc
     3601 tctgggtgta gatgttgaat atgaagagtg tgtagggggg catgaatggc gtctttggaa
     3661 tttatggata aagaaattta tagatataat tgggtagtac acttatggta ttaacaggta
     3721 tactataaaa tatctttaaa tatgataaag actgaaatcc taaaatcaaa ttatgatttt
     3781 aaaatttcag tctataagaa tttaataaaa ttttattaag ttcttacacg agttattatt
     3841 ggagtaaaat tgtcaactat atctatatag tatttactca attcctctaa aatctcttcg
     3901 cataaatccg tactatgacc aagattaata agaaactgtt ttgtaaaatg ctttattaca
     3961 gcttgagaag atatatcgta cttgtgtgaa gtataaactc taattatttc aggtttcata
     4021 gcaagtatac caatacaaga aagtattaat tcatcatgtt ctatagattc tttatcatta
     4081 acatatcttt tcatgaccat ttcggttaca cttctttgat agcttaaaat agcttcttca
     4141 taagctatat ccaaataaaa atttctatat gcttcgtttt ttagaaggaa acaaaggtca
     4201 gcattcatag caagaaagaa catcatcata gtaccttctt catggtagtt atagattaaa
     4261 gactgcattt catttcttat atcttcatat attatagctc ctatttcgct tttacttgag
     4321 aagtaatagc ttattagacc taaatttata ttagctcttt ctgcaatttg cctagttgtt
     4381 gtcttatgat aaccttgctc aaaaaataat tctctagcag cacttataat actagctttt
     4441 gtttctttgc ccttttctga ttttgacata tttactgtgt atacctctct tttcaatctt
     4501 tttttgaaaa tttgctaact ataaatattg taatagaacc acctattata ggaaccaaaa
     4561 ttctgtcaaa tacagtaaga ataccaatca tatttaataa tgccaatata acacttacta
     4621 taacagcata gatagctcct ttttttgtag cgcctttcca gcaaagacca attacaaatg
     4681 gtgcaaatat tacacctgta cataatggag ccggccaaaa caataattct aataaactat
     4741 caaatttaag tgctatgatt gcacctatta tagctgtcaa agctgatgca atttgagaat
     4801 actttagagt attttttaaa tcattaaatt ctatattagg atgtaataat ttgttataaa
     4861 tatctcttga tataatagca ctcattgagg tcattaatga atcggcagta gacattatgg
     4921 cagccaatat agcagctaag aataatacag atattaaagg tggcaaagta gtcgatatca
     4981 tttgaggtaa aacctgaaca gatgatatgt ttggaaataa actaccacca atagctccaa
     5041 ttattcctat agggaaggca agaaataata gtataaatcc tgcaattaca ctacttttaa
     5101 ctgcattttt agcattatca gcacattgat atcttaaaaa actttcataa gaaaatattg
     5161 agcctaaaac taatggtaaa actgtataca taaaatcact aatcgattta tttcctggaa
     5221 agaatggttc ttttaccatt gattgtaaca taagtaaagg gttgtcgcca actaatttta
     5281 agcttatata agctactatt ggcaaaaaca ctatacaaat agcaagttga ataaatgctg
     5341 atactgcaac tccccacatt cctgatgtat aagtatatat aattataact ataactccta
     5401 taattacagc tatatcataa ggaataccaa atgcagtaga tacagaacca aaagaaatta
     5461 attgactacc cataatagct agtatagata ttggtgttac aagactttga taagctctta
     5521 aaaatacact atgaccatat ctagtttcaa tataatcagc aggtgttata tcacaagata
     5581 agcttcttaa ttttcctgcg aaaaaggcat ttataaagca agctaaagct gcacctatag
     5641 tagaatacat tccaacccaa agtcctcttt cagctcccca ttgaactcca ccaactaagc
     5701 accctcctcc aaaagatgta gcagcaatgg ttgcagctag tataggcaaa ggaagatttc
     5761 tacctgcgat taggaaatcg gatgctgttt taactttttt tgaaaaataa aaaccaatcc
     5821 atatgtatat tgcacacgta acaattaaaa gtaacatagt cttttctcct ttataaaata
     5881 ttttaataaa atatataaaa tatatttaaa atatatttta tataaaagta gtacgatatt
     5941 tgttttattg gttttagatt aatatagcta taataattta tgatattaga tggaaattac
     6001 gtaaaaatag aaaattgaaa attctgaaat ttttgcatat tgatatttat ctcatagatt
     6061 cttttttgta attgtaacac gcttttattt ttaaatcaat agtacatgta taaaaataaa
     6121 aggaatctaa ataagaaaa
//
LOCUS       DAELSY010000194         6121 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.195, whole genome shotgun sequence.
ACCESSION   DAELSY010000194 DAELSY010000000
VERSION     DAELSY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.195"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            165..803
                     /locus_tag="KPG09_003470"
     CDS             165..803
                     /locus_tag="KPG09_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0968032.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            810..1652
                     /locus_tag="KPG09_003471"
     CDS             810..1652
                     /locus_tag="KPG09_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBG0968033.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            1713..4340
                     /locus_tag="KPG09_003472"
     CDS             1713..4340
                     /locus_tag="KPG09_003472"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG0968034.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            4554..4799
                     /locus_tag="KPG09_003473"
     CDS             4554..4799
                     /locus_tag="KPG09_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0968035.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            5011..5328
                     /locus_tag="KPG09_003474"
     CDS             5011..5328
                     /locus_tag="KPG09_003474"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0968036.1"
                     /translation="MSKKHKKYSKELKLKAVNLYIKEGYSSYKIAEMLNIRSKTQVQN
                     WIKDYKNKGKTAFNEETRGRFKNISLENDNRKFKSVEEELKYLRMENEFLKKLSTLLD
                     KSK"
     gene            5355..>6121
                     /locus_tag="KPG09_003475"
     CDS             5355..>6121
                     /locus_tag="KPG09_003475"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBG0968037.1"
                     /translation="MSRKYSISSMCKLIGVSRSGYYKWLSYSKKSSDRGIKDRIIKDY
                     IIEIHKKYRGTYGRKRICTYLNKILDSPINHKKVYRLMKELGIKSIIRKKVYRRKFKS
                     YEVYDNILNREFRANQPLEKICMDITYIPIGKKFLYMNVAKDLFNGEIVAYEISTKMD
                     TKLVNKTVNQLINMNLAKDCILHTDQGSQYTSRSYSKRLKDNGIIQSMSRRGNCWDNA
                     PIESFFSHFKSELIYLIDTTDPKEMISLINDYIYFYN"
BASE COUNT         2493 a          677 c         1165 g         1786 t
ORIGIN      
        1 aagaaatatc ccccattaat atataataaa tatctttaag cgagagaact tagtaaaaaa
       61 tatatcgtat tcaataaaaa atctgtttac agaatggtat atatagttat ataatatact
      121 atataagatg cgaaaagata aaaagtatag cacaatgaaa gaaggtgatt attattcagc
      181 ttaatgaaag acaactaaaa attatagata tagttaagga aaatgaacca ataacgagtg
      241 aaagtattgc ctccagcttg aatgtgactc gtgctacact tagatctgat ttagctatat
      301 taacaatgac aggaatatta gacgcaagac caaaagttgg atatttttat tcaggagtta
      361 gtgaaataaa tttaattggt aaaagtgtaa aagaaaaaac agtagaggat ataatgagca
      421 tgcctgtact agctaagaag gatgaaagta tatatgatgt aatagtcact atgtttttat
      481 ctgatgtagg gagtatagtt attatagatg agaatgaaga gttatgtgga gttgtttcca
      541 gaaaagactt gctaaaagca actataggag gttctgatat aaataaaatg ccaataggta
      601 tgataatgac aagaactcca aatgtagtca ctttaactaa aggagcaagt gttttattag
      661 cttcaagaaa aataatagaa catgaagtag attcgattcc gattgttgaa tataagggag
      721 aagacaaaaa tcatatgaga gttgttggga gaatatcaaa aaccaacata actaagctat
      781 tcttagaaat agttgacaat taaggaggta tgctggtgaa aaacctaatt atatatgcag
      841 tatcagattc agtaggggag actgcacaac aagttgcaaa ggcatgtatg tctcagtttt
      901 atgtgaatga aacttatgaa ataaaaagat ttccatacat gattaataaa ggagtactac
      961 ttgaaacttt ggagaatgca aaagctgaaa acgctttaat tgtatataca ttagttgatg
     1021 aagaattatg cagtattgtt gaaagatatt gcgaaagaga aggtcttagt tgtatagatt
     1081 taatgacaga tatactaaga gaaataagca agagaacagg aagaaaacct aaaagagaag
     1141 ctggtataat tagaaaatta gatgaatctt actttaagag agttgaagct atagaatttg
     1201 cagtgaaata tgatgatgga aaagacccta gaggtgttct tcaagcagac ataattttag
     1261 tgggaatatc aagaacatct aaaactcctt tgagtatgta tttagctaat aaaaatataa
     1321 aagtagctaa tgttccatta gttccagaaa taccaattcc taaagaagtg tttgagatag
     1381 atactaaaaa gataataggt cttactaatt cacctgaaaa gctgaatgag ataagaaccc
     1441 aaagattaaa agctttagga ttgtccagta aagcaaacta cgctaattta gaaagaatac
     1501 ttcaggagtt agattattca gaagagataa tgaaaagaat tggatgccca gtaataaacg
     1561 tttcaaataa ggcaatagaa gaaacagcag gtataatttt agacataatg aaagaaaatg
     1621 gtctaaagat atacaaagaa atagaaattt aataatgtct attaataaaa atgatataaa
     1681 ttaaaataaa aagattaatg ggggatttaa caatggaaac taagtatgtt tatagttttg
     1741 gtgaaggaag caaagacatg aaatctttac taggaggtaa aggtgccaat ttagcagaaa
     1801 tgactaagat aggcttacct gtccctcctg gattcactat aacaacagaa gcatgtaatg
     1861 attattatgt taacaatgaa agtataagag cagagataat aaaagaaata gaagagcatt
     1921 tagctacttt agaaaaagac ttaaataaaa cattaggatg caataagaat ccattattag
     1981 tatcagttcg ttctggtgca gtattctcta tgccaggaat gatggataca attcttaacc
     2041 taggtcttaa tgacaatagt gttgttggct tagcagaggc tacacaaaat gaaagatttg
     2101 catatgatag ttacagaaga ttcatacaaa tgttttctga tgttgctatg gaagttccaa
     2161 aatataaatt tgaaaatgta ttagacagag taaaagaagc aaagggatac acagttgata
     2221 cagaacttac aactgatgat ttaaaagaaa tagtaaaaga atttaaagca atttataaaa
     2281 aagaaataaa aaatgatttc ccacaagacc caaaagagca attaatgctt gcaatagagg
     2341 ctgtatttag atcatggaat aatcctcgtg caatcgtata ccgtaaattg aatgatatag
     2401 ctcataactt aggaacagca gtaaatattc aatcaatggt atttggtaat atgggagaaa
     2461 ctagtggtac aggtgttgca tttactagaa atccagctac aggagaaaat aaattatttg
     2521 gtgaattttt aatgaatgca caaggtgaag acgttgttgc aggtataaga actcctcaaa
     2581 atatatcaac tcttgcagat gtaatgccag ctgtattcga tgaatttgta aaaattactc
     2641 atatacttga aggtcattat aaagatatgc aagatataga gtttactata gaaaatgaaa
     2701 gattatatat actacaaact agaaatggta agagaacagc agcagcagca ataaatgtag
     2761 cggttgattt agttgaagct ggaataattg atgaaaaaga agctatcatg agaattgagc
     2821 ctaatcaatt agaccaatta ttacatccaa aatttgaaga taaggcatta aaagaagcca
     2881 aggttatagc aaaaggttta ccagcatcac caggtgcagc aagtggtaaa gtttatttca
     2941 atgctgatga tgtagtaaaa gcaaatgaaa aaggagaaaa agtagtttta gttagattag
     3001 agacatctcc agaagatata gaaggaatgg ttaaagcaga aggaatcctt acagctagag
     3061 gtggaatgac atcacatgca gcagttgttg ctagaggtat gggtaaatgt tgtgttgctg
     3121 gatgtggaga aataaaagtt gatgagttta ataaagaaat aagagcactt gatgatgtag
     3181 ttataaaaga aggtgaatac atttctatag atggttctac aggaaatgtg tacctaggag
     3241 atgttaagaa aacagaagta tctttaactg gaaactttga aaaattaatg aactgggtag
     3301 acaaacacaa atgtatgatg gttagaacta atgcagataa tcctagagat gctcgtgcag
     3361 ctatagagtt tggtgcagaa ggtataggtc tatgtagaac tgagcatatg ttctttgatg
     3421 aagctagatt gccagcagtt agagaaatga tattatctaa tacagtagaa caaagagaaa
     3481 aagctcttga aaaaatatta ccaatgcaaa gagaagattt tgtagagtta tttaaagtta
     3541 tggatggaaa accagttaat ataagattac tagacccacc actacatgaa tttttgcctc
     3601 atgatgatga gactatagaa gaactttcaa aatctatggg aataaaagta agtgatatta
     3661 agaagagaat agttgattta gatgagttta acccaatgct tggacacaga ggttgtagac
     3721 ttgcaataac ttatcctgaa atatgtgtaa tgcaatctaa agctataata caaggtgcta
     3781 tagaagcaat caaagcaggt gttaaagttt ctccagagat aatggttcca ttagttggag
     3841 aagtaaatga gttaaaaata ataagaaaaa tgatagtaga aactgttgac gctataataa
     3901 aagaagaagg tatagaagtt ccttatactg taggtactat gatagaaata cctagagctt
     3961 gtttaactgc tgatgaaata gcacaagaag cagacttctt cagttttggt actaatgact
     4021 taactcaaat ggcatttgga tattcaagag atgatgctgg taaattctta ggtcaatatg
     4081 tagatgaaga aatattagaa aaagacccat tccaagtact agaccaaaat ggtgttggta
     4141 agttagttaa gatgggagct aaattaggta gagaagttaa accagaatta aaactaggaa
     4201 tatgtggaga gcatggagga gaaccatctt cagtagagtt ctgttatagt gtaggactta
     4261 actatgtatc ttgttcacca tttagagtgc caattgctag acttgcagca gctcaagcat
     4321 ctataaaaaa tcctagataa taatagactg caaaatatgt aactaaataa aaataaaatc
     4381 ccctattgtt caatgtatca aattaagtat ttgtaaattg aatatagggg atttttatta
     4441 taaaaattta acttatacag ttgtaagtga aacgtattta attatattgc atatattatt
     4501 gtagagagaa atttcaacta tcaattaaat tgagtttact ggaggggata aagttgacaa
     4561 agtgtcctaa atgtggaaag gaagtatcat cacgtccagg tagtatatgc ccacgatgtg
     4621 gatttaaagt agcagatgag agtctaaaaa taagatgtcc agaacctagt tgtagggctt
     4681 tggtatctag gaaactggat tattgtccta aatgtggatg caaacttaga ggatataata
     4741 tttctgaatt tgttaacatg gtaagatgta aaatagatga gacatttgat aaaaaataat
     4801 taaatttaga gttaagcttt tgagggtgtc tcaaaatgaa ctttttagtt cagaaggcac
     4861 tctttttgta tggaatcaaa tatattttta taatttaaac aataaaaaag aggcttatct
     4921 ctattttagt ataatcccct tatagtagac atttaaataa aaggctatta taataagtct
     4981 aaaaagcgta tactctaagg gggtttttct atgtctaaaa aacataaaaa atatagcaaa
     5041 gaacttaaac taaaagctgt aaatttatat ataaaagaag gatactcatc atacaaaatt
     5101 gctgagatgc tgaatataag aagcaaaaca caagttcaaa actggataaa agattataaa
     5161 aataaaggta aaactgcatt taatgaggaa actagaggca gatttaaaaa tattagtcta
     5221 gaaaatgata atagaaaatt taaatctgtt gaagaagaat tgaagtactt acgcatggag
     5281 aatgaatttt taaaaaagtt aagtacccta ttggacaagt caaagtaagc gataaattta
     5341 aaacaattaa acttatgtct aggaagtata gtatttctag tatgtgtaag ttgataggtg
     5401 tgtcaagaag cggttactat aaatggctaa gttattctaa aaaatcttcc gataggggta
     5461 tcaaagatag aattattaag gattatataa ttgaaataca taaaaaatat agaggaactt
     5521 acggtagaaa aagaatttgt acttatttaa ataaaatact tgatagccct attaatcata
     5581 agaaagtata taggttgatg aaagaactag gaattaaatc tattattaga aagaaagtat
     5641 atagacgtaa gtttaaatca tatgaggtat atgataatat attaaatcgt gaatttagag
     5701 ctaatcaacc attagaaaaa atttgtatgg atataacata catacctatc ggtaaaaaat
     5761 tcttatacat gaatgtagcc aaagatttat ttaatggtga aatagtggca tatgagataa
     5821 gtacaaaaat ggatactaaa ttagttaata aaacagtaaa tcaactaata aatatgaatt
     5881 tagctaaaga ttgtatttta catacagatc aaggttcaca atatacaagt agatcttact
     5941 ctaaaaggct taaagataat ggtattatac aatctatgtc ccgaaggggc aactgctggg
     6001 ataatgctcc tatagaaagt tttttcagtc attttaaatc tgaattaata tatttaattg
     6061 atactacaga tccaaaggaa atgattagtc taataaatga ttatatttat ttttataata
     6121 a
//
LOCUS       DAELSY010000195         5980 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.196, whole genome shotgun sequence.
ACCESSION   DAELSY010000195 DAELSY010000000
VERSION     DAELSY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.196"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(418..1134)
                     /locus_tag="KPG09_003476"
     CDS             complement(418..1134)
                     /locus_tag="KPG09_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0968038.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSREIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            complement(1675..2475)
                     /locus_tag="KPG09_003477"
     CDS             complement(1675..2475)
                     /locus_tag="KPG09_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG0968039.1"
                     /translation="MTFKNKKFTINKYEILSIILVLCLILTGFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNVGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            complement(2790..3521)
                     /locus_tag="KPG09_003478"
     CDS             complement(2790..3521)
                     /locus_tag="KPG09_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968040.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(3508..4203)
                     /locus_tag="KPG09_003479"
     CDS             complement(3508..4203)
                     /locus_tag="KPG09_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0968041.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(4205..4996)
                     /locus_tag="KPG09_003480"
     CDS             complement(4205..4996)
                     /locus_tag="KPG09_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0968042.1"
                     /translation="MKNKFFTTLLTGFFAIVFFFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            complement(5006..5818)
                     /gene="modA"
                     /locus_tag="KPG09_003481"
     CDS             complement(5006..5818)
                     /gene="modA"
                     /locus_tag="KPG09_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0968043.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
BASE COUNT         2080 a          844 c          729 g         2327 t
ORIGIN      
        1 aaattgtaga ttattaaatt agtgttgtaa aacattttat tacttgtaaa tgcatgaaac
       61 cttaaggtta atagtatcaa agttaatata agtgtaagtt ctatttttac cttagaaaaa
      121 aggcttatga cattctctaa gaatttgact ttagtctgtt tatttttttt atacaatgta
      181 aaacatagta tagtcaataa aaaacttgat aatataatac ctatgcagac aaactctaat
      241 tctacttttg acaaatgtac aggagtaccg aaaaaataaa aataagggag agcaaactct
      301 ttataataac aattaatatt ataagaaaag ttattattca aatcataaac tgcttcaaat
      361 aatatagaaa ctaatagaaa cagtaataat aataaaataa attttgcttt agaatcatta
      421 ataattaagt ttttcaattt tgtaaccaat gccccacacc acctttaaat attttggttc
      481 ttttggatta atttcaattt tttctctaat tcttctaata tgaacagcaa tagttctctc
      541 acaactataa gcatcttcat tccagacatt ttcataaatt tcacgggaag agaaaatttt
      601 accttttcgt gacataagaa gtgtaagtat tttatattca agtggtgtca ttttaatttc
      661 ctcaccatct aaagaaacta tttttgtatc tgtatttagc tctaggccac cactttttag
      721 aatataatta gtatcatttg agttatttgt attactaaaa tcacctaaat ttatatacct
      781 tctaagttga gattttactc gagcagtaag ttctagtgga ttaaatggtt tagttatata
      841 gtcatcagca cccatattaa gacctataat tttgtccata tcttctgatt ttgcagataa
      901 gagaattatt ggtatattcc ttttttctct tattttaagt gtagttttaa gcccatctaa
      961 attgggcatc attacatcca ttattattaa cttaatatca ttttcgatta gtatgtccaa
     1021 tgcttcaagt ccatcatatg cctttaaaac attaaagtta tcaggtctca aatagaactc
     1081 tatagcatca actatctctc tatcatcatc tacaaccaat atattttgat tcaatttatt
     1141 aacccccttg aaattttatt tttaaccaat ttttatttta taccaaaaag aggtattaga
     1201 aaacagtaag cccgttcatt atgaaataaa aactagtact tttaatatat tgtcaatgca
     1261 tatagtaaat ttaaaagtat agacgcccat attaatctga aggtaaaagt ttatgaaaat
     1321 atctagggta atctatcaca ctccttatgt tattttgggt ttattaattt tttaggttta
     1381 aacatatatt aacaaatata aataacaaat gagtttcata attattaaga atttataaca
     1441 aatatttatt ttaatgtagt ttaatataaa taggtagttt aatgtagata tgtataaagt
     1501 atttaataag gcttataggt tttaatgatg atatctttat atattttaag agatgatgaa
     1561 attagatatg ggtaatagtt gaatgagaaa aagtagtata gcacaaaaag tgctatatca
     1621 caaaatctca aataatttcc ttcttataac ttaaattaaa agaataaata tttcttataa
     1681 attaggtgta tgaagtatag caggaacatc tttattattt aataacttat caaatgattt
     1741 atggtcagac ttatagccaa taccttctct atattctaat tcatcaacaa ttttgtattg
     1801 ttgacaggca tttttaacct tttcatagtc ttcagtagaa tgagctttat aaactatctt
     1861 attattaaaa gttattttat cagcaaattt aggttttaca aaatatttag catcttttat
     1921 tttaccaaca ttatataaat tttggataac agaaccattt tttaaatgtt gtaaatggtc
     1981 atctaactta taagtatgtg ctatatgagt tacattttta aatttttctt caaatgtaag
     2041 agcaattttt tccatgtaat tgaagtcagt attaccattt gtatttgttt ctggtaataa
     2101 tattatattt tcccttatta caccaaggct attaagagaa gctaaaaatt ctctatttct
     2161 atattctgat ttttgtatat ttgttaggtt ttcatatttt ttatatttat caaagacgga
     2221 tataccacat ccagtagata ctaaaactac aaatacatga ttttttcctc tttcttttat
     2281 agcattaaca atagcactcc cagcccacaa cacttcatca tcttgatgct gaacatagaa
     2341 gacaacataa tctttaaatt ttttatgact ataattcaca tagctactaa aaccggttaa
     2401 aatcaagcat aaaactagaa taatagatag aatttcatat ttatttatag taaatttttt
     2461 attcttaaat gtcatttaat catctcctga aaagttgtct tagttatatt atcaaacaaa
     2521 gtactaaaaa ttatttctta aatgctgaaa aatatagagt agattaatgg aataaaattt
     2581 tttttattag tttaaaataa agaaaacatt tatgtataag actaatcaaa atattaataa
     2641 ttgataggaa ttgagattta aagggattgt acatttattc gggattcatt ttaatagaat
     2701 tttactttag tacttagaat aaaagtaata actttttaaa tttaaagagt acacatatat
     2761 atttgtgtac tccttgatga tatgattgct tatttactac aaaaacagag tctttttaaa
     2821 cctaaaatat ttgatgcacc agctatcgta gttccataat agtaaactgg tttgttaagt
     2881 gatagaaaat ttataattga gttattacat aatgtacttc ctgtgcaaat cactaaatca
     2941 gcccaagata ttacatcttc ataatctcgt ataccatctt caatcttaac attatattga
     3001 atagtatcca caaattcagg atttaaatct aatactcttg tttcgaaaaa gtccttaata
     3061 ttatcaatta tggctggttg atatcctata attgctactt ttggattacc aaattccatt
     3121 tttataaact cagggaattt ttttgcacat acctctggct cgttattttt acaatgaatg
     3181 gttctatccg tcttgcctaa gtatttcata actgcattta atgatgctat aaaaagactt
     3241 ctatcatgag gattgtcatg taaatctaat gataatatgt cttttaaaga accttcaaat
     3301 gtagatggag tagaagtaaa tgcttgtcct ttagctcctt taaagtcagc ttcaagcatt
     3361 atttctttac caactataat tgggaaatct ttacgcttag tatttccaat tgcttctttt
     3421 gaagatagag atttggacac aatacttatc atatccatat ttaaattata tttatcgact
     3481 tgtactaata gataatctct caaaatatca aatatattca tagaaaaaag attctccttt
     3541 attttttatt tcttcattta aatcaaaaat atgatttatt tttccattgt tcattattcc
     3601 tatcttatga gcaaaaaaca ttgcttcaga aatatcatga gtaacatgaa tcactgttgg
     3661 tttgtatttt atgtagacat ccttcaaaat tttgcttata tgtttttttg taatataatc
     3721 caatgaactc ataggttcat ctaaaagtaa tatatcaggt tcagctatta aggcacgtgc
     3781 aaaagcaact cgttgctttt cgcctccact taaattttta ggatacctat tttttatatc
     3841 ttctatctgc attaatttag acaagtggtt agccttaaga atctgttcat ctataggtat
     3901 ttttcttaat ttaagtggaa accttatatt atcttcaaca ttcatatttg gaaataattc
     3961 aaaattttga tatacaaatc ctatatttct attttctgga ggtaagtttg atattaaatt
     4021 ttctttgtat gatacatttc ctttagcaga tggatatctt cctgctaatg tttctaggaa
     4081 tagagtttta cctgacccac tagctcctaa taaaacaaag tattcatgat tattaatatt
     4141 taaactattt tcttgtaatt taaaattagg taaatcaaga gataaatttt ttatttttaa
     4201 catattaatt cttatcaacc tctctattaa aaaatatctc aaaaagcatc aagcatgtaa
     4261 gtgagataaa tatcaaaatt acagctattc cgattgcaat gtctatatca ccagtagaca
     4321 tatttaaata tatagatgta ggaataattt cagttttcat acgtgtagaa cctgctatca
     4381 tagctactgc accaaattca ccaagtgctc ttgaccaagt cattacagtt gcagatatga
     4441 ttccagtttt tagatttgga agaattataa atctaaatgc ttcccaacta ttacatccaa
     4501 gggtacgtgc aacaaatatc atcttttcat tactttcatt tagtgaagtg tgaagtattt
     4561 taatagtaag tggaagattt acaaatactt gtgctaaaac tataccttgc tttgtaaata
     4621 taaaatcaag cttaaaaatc ttataaatgg aatctccaat ccccattctt ccaaacaaaa
     4681 gtaaaagagc aattcctgat actaagtgtg gaagagacat agggagattt attatctgta
     4741 taatcagttt tttaaatggt aatttagtaa tatgtaaaaa atatgctact ggaatagcta
     4801 ataaaaggca tactatagta gatattaaag atgttttaat actaagtgtt attgcatact
     4861 gtacttccac ggattttaag catataggaa cataacttat ccctttaatg attaacataa
     4921 gaagtggtga tacaataaaa aaaaagacta tagcaaaaaa tccagtaagt agtgtagtaa
     4981 aaaatttatt tttcaagttt aatcactact taacaggttc aaaaccatat tttttaaata
     5041 tctgtttagc ttcatctgac tctatataat ttataaattt attagctaaa tcttttttat
     5101 cactatagtt tacgattcct gctgatatga gttggtctat atttttatct tgggcaattt
     5161 ctataacttc tacatcttca ttgccaaaga ccgaatcttt tgtggctatt gtagcatcag
     5221 ctttacttga tgttaactgt acaatcattt catttacagt tgcagctgta gagacaacat
     5281 tatcatttat agattttaaa ttattttttt caagtatttt ttgtgtagtc ttacctatag
     5341 cattagactc tttgtctccc aaaataagtt taactccatc tttatttaag tcttccaatt
     5401 tttgaatatt agctggattt cctttaggta caactattgc aggtgtatga tgtgcaaccg
     5461 ttttatgttc acctacaagt tctttttcaa aggcctttga ataaattggt tcagaaccaa
     5521 caataaatac gtctcctttg tgacttgtct caatttgagc tattagctgt gcagaacctg
     5581 catagctata ttcaactttg acattatttt ctttctcaaa tttttttgct atttcatcca
     5641 ttggtttctt taaacctgcc cctgaataga caaaaagagt ctctttttct tttgaatcat
     5701 cagctgttgt cactttagta ttagattcat cattagcttt gttttcttta gaattacatg
     5761 cagtaatact taatgaaata atcatagcta aagctagtac tgaaagtttt ttaaacataa
     5821 gatattcccc cttagtaaat tagtgtaaat tcttttttag tatatcatac taagaaaacg
     5881 atgtctaagt ttttgttatg ttttataatg ttttgttatg ttttattatg tatagttaag
     5941 aaaaaatcta tttataatgt catttccata ggtagttatt
//
LOCUS       DAELSY010000196         5977 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.197, whole genome shotgun sequence.
ACCESSION   DAELSY010000196 DAELSY010000000
VERSION     DAELSY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.197"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            117..1469
                     /locus_tag="KPG09_003482"
     CDS             117..1469
                     /locus_tag="KPG09_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0968044.1"
                     /translation="MTVTITGWIILSFLFLCIIIYILYSKAKSQKTIEKVAYTDFVTG
                     YSNWRKFELDVTNLLKKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLN
                     ELTNINETFARVSADNFNILLTYNKKEDIINIIKKIIANNELVNLSFGIYEIKDKDLS
                     VSVYSDRASLAKSSIKNNSDVNFAFFNDKLREKLLLEDKIEKEMEYALESGQFVMYLQ
                     PKYNIKLDKFCGSEALVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIR
                     SLFDKGISPLPISVNFSRVDFLKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTD
                     TLFNVSRNLQDIGFIVAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIV
                     IKSIVSLIKQLGIRVVAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI
                     "
     gene            1502..4939
                     /locus_tag="KPG09_003483"
     CDS             1502..4939
                     /locus_tag="KPG09_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG0968045.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPIFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEISKQL
                     YKNKTIELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENILGGIIITEFNDDFTIKYTNSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIIDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSEMVKTISNSIIHKDNKEVFLNTFLCKNLLSAYNKNITEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIKLKLKAERDTLTGLYN
                     KGTTENLINDFLNSDEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKHLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENTKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNNCIYQNELENNISDNNIKLANK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSER
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            4957..5703
                     /locus_tag="KPG09_003484"
     CDS             4957..5703
                     /locus_tag="KPG09_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBG0968046.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNSIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5652..5972)
                     /locus_tag="KPG09_003485"
     CDS             complement(5652..5972)
                     /locus_tag="KPG09_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG0968047.1"
                     /translation="MEKIMNFLKENSTFYFATVEDNKPRVRSFGFIMDYEGKLYFGIV
                     KYKTSYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAK
                     NSLV"
BASE COUNT         2501 a          595 c          894 g         1987 t
ORIGIN      
        1 gattaataat aaagaatata tcaatagcat ttatagtact attctttttt agtgtattta
       61 cattctttta tataggtaat attagtagag tattagaata tgaaacaaat gacataatga
      121 cagttacaat tactggatgg attatacttt catttttatt tttatgtatt ataatttaca
      181 tattatattc aaaagcaaag agtcaaaaaa ctatagaaaa agtagcatat acggattttg
      241 taacaggtta ttccaactgg agaaagtttg aattagatgt tacaaattta ttaaagaaaa
      301 catcacaaaa taataaatat gcaatggtca tattcgatat tgataaattt aaagcaatta
      361 atgatatata tggacataaa aagggcaatt taatattaaa agatattgca gatactttaa
      421 atgaattgac taatatcaat gaaacttttg ctcgtgtaag tgctgataat tttaatatat
      481 tattaacata taacaaaaaa gaggatataa taaatattat taaaaaaatt atagcaaata
      541 atgaacttgt aaacttatct tttggaattt atgaaatcaa agataaggat ttatctgtaa
      601 gtgtatatag tgatagagct tcacttgcaa aatcatcaat aaaaaataat agtgatgtaa
      661 attttgcatt ttttaatgat aaattaagag agaaattatt acttgaagat aagatagaaa
      721 aagaaatgga atatgctctt gaaagtgggc aattcgtaat gtatttacaa ccaaaatata
      781 atattaaatt agataaattt tgtggaagtg aagccctagt cagatggcaa tatacagaaa
      841 aagaagtcat atatccaggt gattttattc ctatatttga aaaaaatgga tttattagaa
      901 aaattgatat gtacatatta gaacaagctt gtaaagaaat ccggtcttta tttgataaag
      961 gaatttcacc tttacctata tctgtaaact tctcaagagt tgatttttta aaaaaagatt
     1021 ttattgaaaa catagtaaat atatgtgata gatataaaat accatatagt ttaatagaaa
     1081 ttgaaattac tgaatctagt atgtttggag atactgatac tctatttaat gttagtagaa
     1141 acctacagga tattggtttt atagttgcaa tggatgattt tggctcggga tattcttctg
     1201 taaatatgct taaaaatata ccattaaatg taataaaatt agatagagga ttttttgttg
     1261 atgataaaga tgttgataag agtcaaattg ttataaaaag catagtttct ctcattaagc
     1321 aattaggaat aagggttgtt gctgaaggaa ttgaaacaag aagtcaaata gaaatgttaa
     1381 aaaaagcaaa ttgtgatatt gttcaaggat attatttttc aaaaccatta ccaattaaag
     1441 aatttgaaaa attagtctat aaaatatagt cagcgagtct taaatgggag gtggtgatta
     1501 agtgataaga gcaaaatcgt taaaaaatgt actgagtatt gttttaatat ttttatttac
     1561 aataatttct ttttctgatt ttgcatttaa aactaatgtc caggaagaag aaaatagtaa
     1621 aattaattta agggaaatat ccctacaaag ttccaatgct ataaaaataa aattagatag
     1681 ttctctatcc tttattaaaa gtgtggcaaa tatggtttct tattttaata ctatagataa
     1741 ctcaaaaata gttccattat tagctaacat atcaaaagaa tctgactttc aaagaatctc
     1801 tattgtatct cctgatggaa taagtcatac cagcgatggc aaaattatca atgtgtcaaa
     1861 atatcagtat tttaagaaag ctattaaagg agaaagcaat atatctgaag ttgtgaaatc
     1921 tccaattgat ggaaaagata cttttgttat agctactcct atatttaata agaataacaa
     1981 aataattggt gttttatacg gttcttacta caatgataaa ttagctgaat ttatagatat
     2041 aacaagtttt aatagtgaag ggtacataga tatatttgaa tctagtggtc aatttgtttt
     2101 aaaatcttca catacaaaca aactgattaa gtcaagtagt aacatatatg atatatttaa
     2161 aaaagcttct tttgatgatg gttatagcta taaaaaaata tatgaagata caaaaaataa
     2221 caagagtggc tttacctcat atgaatacaa aggagaaaat cgttatgcta cttatacacc
     2281 cattggtgta aatgactggt atgtaatttc tgtgatacca caatatactg taagaagaaa
     2341 cattgataaa ataaatcaat tagcattttc tcttattttc aaagtattct tattgtttat
     2401 tatgcttata atatatatta tttataaaaa caataaatct cagaaagata tactaaaagc
     2461 taacaaagaa atctcatcta ttacaaatag cattcctgga ggagtacaaa agtgtaccct
     2521 gggtgaaaca tgcaatatta attttttaag tgatggattt atagagttgt ttggttatac
     2581 aagagaagaa ataaaaacat tgtttaataa tgatttttat agtttaatac ataaagatga
     2641 tgttgaaaga ataaaaaaag aaatttctaa acaactttac aaaaataaaa ctattgaatt
     2701 agaatatagg attataacaa aaaatggtaa tacagtatgg gttttagata aaggagaact
     2761 tataacagaa cctgatggaa aagaatattt ttgttgtgta actgtagata ttactagaac
     2821 aaaacaatta gaacaagaga tggaacaaaa aaatctagaa cttgaaatga tatcagaaaa
     2881 tattttaggt ggtataatta taacagaatt taatgatgat tttactataa aatacactaa
     2941 ctctggttat ctttcaatga ttggatatac acgtgaacaa ttagaaaatg aacttaaata
     3001 caaagcttgt aatcttgttt tttatgaaga tagaaagaag gctcttgaaa gtatacaaca
     3061 tcaattaaac acaaaaggaa tagctgtata tcaacataga ataagaagaa gagatggtaa
     3121 tattatctgg gtttctataa aaggaaggct tattattgat gatgatggta ttgaaaaagg
     3181 tatttggatt ctaaatgaca taacagattt aaaaaatgca gaacaaaaac ttaaaataaa
     3241 tgaagaaaga tttagaattg cattaaagca aacatcaaat actatatttg agtatgatat
     3301 aaaaacaaag tcacttttaa atactagtaa agagcttgat aaatataata tttataaaat
     3361 tactaaaaac gttccatatt ctctagtaga atcaggagtt attcataaag attttagtga
     3421 tgattttata aacatgtatg aaaatgtaat aaatggagca aaaaaagcaa gttgtgtagt
     3481 taagatgaaa cttccagatg aaaattatat atggaataaa gttaccttga ctactatttt
     3541 tgatgaagat ggtaatcctg taagagcaat tggtatacag gaagatataa gcaaacaaaa
     3601 ggaagcagag ataagatata atcaagaagg taagtatcat tcagctatat tgtcggaagc
     3661 aatgataacc tatgaaataa atgtatcaaa agatactttt atcaaaggaa atgaaagttg
     3721 gcaaaaacca tttggaatta agcctgacaa taattattct gaaatggtaa aaactatatc
     3781 aaacagtatt attcacaaag acaataaaga ggtgttttta aatacatttt tgtgtaaaaa
     3841 tttattatct gcatataaca aaaatataac tgaaataaaa ttagaatatc gaagaatgaa
     3901 tcaagaaaat aaaatgattt gggtcatttg cacaatgcac ttaataaaag attctattac
     3961 gaatgatata aaagcacttg cttatgttaa agatattgac aagcaaaaaa atgaagaaat
     4021 taaattaaaa ttaaaagctg aaagagatac tcttacagga ttatataata aaggtactac
     4081 agaaaatttg attaatgatt tcttgaactc tgacgaagca agcttaggaa tgcatgcatt
     4141 tttaattgtt gatatagata attttaaatc tgtaaatgat aatttaggac atgtatttgg
     4201 agataaagta ttatttgaag tttctaaaca cctaaaacct atatttagaa aagatgatat
     4261 agtaggtcga attggtggag atgaatttat agtattctta aaaaatattc agtcttttga
     4321 gaataccaaa aagaaagcag aaaaaatatg cgatatattt agaaattctt atacggaaaa
     4381 taaaaagaca tataaaatat ctggaacagt tggaatttca tttgtaccag aacatggaac
     4441 tgtatttgaa gaactttata aaaaagcaga catagcactt tattatgcaa agagcaaagg
     4501 aaaagataag tatgcacttt ataataattg tatctaccaa aatgaacttg aaaataatat
     4561 aagtgataat aatataaaat tagcaaataa atgtgagaaa tccaaaccag tacttaaaga
     4621 aattcttgat tctattgata attatattta tatagtgaat ccaaatactt atgaacttct
     4681 ttttgtcaat aataaggtgt ctcgtttgga tttagagata aaattaggag aaacctgtta
     4741 taaatcacta ttaaaatctg agagaccatg taataattgc cctcttaacg gattagaaga
     4801 aaataagcat tcaaaattca aaatagagac aaatacattt ggtaaaaata tcagtatagc
     4861 aagttggatt aattgcatag atggagaata caactgtctt atagagtgta tagattcttc
     4921 tttatacaaa aataaataaa aaaggactat gaagatatgt taaatagtat aactttgata
     4981 cttggtataa tatttacaat ttactatttt tatctcaaaa tagtttttgg aggaatttca
     5041 tttagtgaga ttttcttagt tgtaggaatt gtactaatta tctatcaaat atttaagaaa
     5101 aaaattaaag aaaagaaggt tttttatagg gtgttaaaaa taataatcag cattttttta
     5161 atagtatttg tagttactga gagtttaatt attttttatc ctaaaaatag tttagaatca
     5221 aaaagtgatt atctcttaat tttaggagct tctgtaaaaa agactacacc aagtacaacc
     5281 ctgaagggta ggttagacac tgcattgaaa tacctaaaaa ttaatgataa ttgttatgtg
     5341 gtagtatctg gtggtaaagg aagtggagaa aaaataactg aggcaaaagc tatgaaagat
     5401 tatcttataa aaaatagtat agataaaaat aggataatag aagaggataa atccacgaat
     5461 acttatgaga attttaaata ttcaaaattg aagatagaag aacatagtca aagaaaacta
     5521 agtgatttaa aagtaaaaat agtaacaaca gattttcatg ttctcagaag taaaatatta
     5581 gcatatagaa atggatataa aaatacgtct ttttatgcta gtaaatcaaa actttcattt
     5641 gtaccaactt actatacaag agaatttttt gcattatgga aaacaatcat ttttgataga
     5701 taataaataa aaagactaga tataaactca tatctagtct tttctattcc cttaattcta
     5761 atccattttc caactttact tgttgtagaa atttcaacat tgggatttac aagtaattgc
     5821 ttataagaag ttttatattt tacgattcca aaatataatt ttccttcata atccattata
     5881 aatccgaatg atcttactct tggtttatta tcttcaactg tagcaaaata gaatgttgag
     5941 ttttctttta aaaaattcat tattttttcc atgatta
//
LOCUS       DAELSY010000197         5902 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.198, whole genome shotgun sequence.
ACCESSION   DAELSY010000197 DAELSY010000000
VERSION     DAELSY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.198"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            114..734
                     /locus_tag="KPG09_003486"
     CDS             114..734
                     /locus_tag="KPG09_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBG0968048.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(900..1436)
                     /locus_tag="KPG09_003487"
     CDS             complement(900..1436)
                     /locus_tag="KPG09_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG0968049.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(1549..2223)
                     /locus_tag="KPG09_003488"
     CDS             complement(1549..2223)
                     /locus_tag="KPG09_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBG0968050.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            2426..4300
                     /gene="asnB"
                     /locus_tag="KPG09_003489"
     CDS             2426..4300
                     /gene="asnB"
                     /locus_tag="KPG09_003489"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG0968051.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEYVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(4392..5828)
                     /locus_tag="KPG09_003490"
     CDS             complement(4392..5828)
                     /locus_tag="KPG09_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG0968052.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVHFTRY
                     IGVDDSIDKYIEKVKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWM
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIAQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
BASE COUNT         2267 a          685 c          861 g         2089 t
ORIGIN      
        1 aaacttatat agattattta ctttttaata ttagattcat tttttgagat tggtatataa
       61 taaacatata gagtatataa taatagttaa agttaaaaaa ggggaaaagc aatatgcagt
      121 atatacaaga attacttgat ttgactcaaa attattgggt gttagccaca ataatcggct
      181 tattaagtgc atttatagaa agctttatac cagccttacc acttgttgct atagttacag
      241 caaatgctgc aatacaagga ttatttattg gatgtttgtt atcatggatt ggctcaggac
      301 ttgggacaac ttcattattc ttattaataa gcagatttaa tgatagtaag ctttttaata
      361 agttgagaaa tagtaaaact gaaaaagcaa taagttggat ggataaacag ggatttaagt
      421 tattatttat agcgtatgcc tgtccattta tgcctggttg tttagttact atcgcatcag
      481 cttttggtaa aaaagattta aaagattttg tgccagcaat gttggcagga aaatttgtaa
      541 tgtttatagt tataagttac gtggcaagtg atatagaagg ttttataaca agtcctttaa
      601 agatagcatc atttatatta ttggtgtttt tgtcatggaa gattggaaat agagtaaata
      661 aaaatcttga aaatcataat tatgatttcc atcataaaaa acatcacaat gataaagatg
      721 ataaaatgat ttagcttgta cgaaataaaa cgtatcttaa aatgatttag tactaaatta
      781 ttttaagata cgttttattt tttgtattaa ggtacaaaat ttttagttct tttgtaaact
      841 aaaatatata aaacttattt atacttagtt caaaatattg ttatttattt tttctacaac
      901 tataataaaa tataaaattg atatccaatc aaaaaaataa ctatgaaatt taatagctta
      961 gatatgaatt tttctgtttt tgagttagtt tttataggta gtaaagaaat gtttatggta
     1021 atgggataaa acaattcaat accttcttta gtaataaggt caagacacaa gtgagataga
     1081 tatcctatta taaacccact ggacaaacat gagaaaacta tattattgtt agtgaagatt
     1141 gtcagagatt taaaaaaact ataaataagt aataaggcaa ttagactgtg agtaaaccct
     1201 ctatgtctaa acctacttcc aaataatttg tatattaaca taaatttttt acttatgtaa
     1261 gaacctctca tatctatatc tggaaataaa gaacctaaat atgcaaaata aatataaatt
     1321 ataattaata ctattttata tgcaatatcg tatttaacta ggtacagatt gtttataaag
     1381 gggagtatca aaagtgcgaa tatataacct cccctttcat gtgtttcttt tatcatataa
     1441 aaatcctcct gaatcgtcac tataaacatt atattggtaa atttagacta tcttttatta
     1501 tattatatag caaacataag tttttttcaa aaggatttta atatttaatt aatcttgaga
     1561 ttttaaattt ttgtaatttt ctaaagcacc attccaattt actacattaa accagttgtc
     1621 aatgtattca tttcttctat tttgatattt taaatagtaa gcatgctccc aaacatcaag
     1681 tcctattata ggagttaggt ttttacttac agggctatcc tgatttggag tagtcataat
     1741 agataatttc ccatctttag tagctacaag ccaagcccaa ccagaaccaa agacatctaa
     1801 agcagatttt tggaactctt gcttaaattt ttcaaaagaa ccaaagtctc tatcaatagc
     1861 ttcttttaaa gattcagaag gtatggtttt ttctggcgtc attatatcaa aaaagaattt
     1921 atgattataa gctccacctg cattatttct tacagttgta gcaatatctt taggtaaaga
     1981 atctaaattt tgcaataatt cacataaaga ataattataa agctcaggat atttttcaag
     2041 agcagcattt aatttatcaa cataagcttg ataatgctta tcatgatgca gtttcattgt
     2101 ttctttatct atataaggtt caagtgcatc atatgcataa ggtaatggtt taaccttaaa
     2161 tttgttattt tcaggtgtaa aagcaaatga agttatgcac tgtgagatta taaataaaga
     2221 cataataact ggtattaata tttttttctt cataaaaatc acctcctaat atagtatttc
     2281 tagaaataaa aaaaatatga aatattaaca gataatttat aagaatttaa tagcaatttc
     2341 attttaaatt gaataagttt taaagccaaa gacaaaacta tgaaatatag aataagacta
     2401 aaggacagaa aggaattttg attatatgga cggcaatatt tcaatttatt ataaaggtga
     2461 tatatctaaa aaagaagata ttaaatttaa aataaaagaa tctgtaaata atacaagact
     2521 ttctagtaga atagagtata aggatgagga aaaaactttt gattttgaaa gatttaatgt
     2581 aattgatgct atttatggaa ataaaccatt tgaaaaagat ggaaaagtta ttgtttttaa
     2641 tggaaatata tataactatc atgaaataaa agaagaatta attaaaaaag gacacaattt
     2701 tactacaaat gatgatattg aagtcttatt ggcatcttat atagaatttg gtaagaattg
     2761 tgtacataag attaaaggaa tgttcaactt tataatctat gatagagaaa atgaaagtat
     2821 atttggagca agagatttat ttgggataaa accactttat tatataaata aggaaaatgc
     2881 aataattttt tcctcagagt ataagttttt acttgaatat atgaaaaatt taaatataaa
     2941 tgaaagaagc ttacaaagtt atttttcatt tcaatatgta cttcctgaag atactatgat
     3001 acaaggaata aggctgatac cagcaggtca ctattttagg gtagaaaatg gtatactatc
     3061 tttaaagaga tataataaac ttgagtttag aagtagtaca aaattttttt atactaaaaa
     3121 tcacttaggc aatagagatg tgaatgagga tgatataaga aatatagtag tagattcaat
     3181 agtaactcat atggaagagg agaaagaaat tggaacattt ttgtctggtg gaatagattc
     3241 atctattata actactgtgg cttcacaaat taatccaaat ataaaatctt tttctgcagg
     3301 ctttagtgtc aaaggatata gtgaattaga agtagcaaag aaaacagctg ataagttagg
     3361 tatagaaaat atccagatta atataacaca agatgaatat ataaaatcat taccgaatgt
     3421 aatttattct ttggatgacc ctatagcaga cccttcagaa gtaggtcttt atttcttaat
     3481 taaagaggca ggtaaacatg ttaaagtagc attgtctgga gaaggagcag atgaattatt
     3541 tggaggatat aatatatata aagagtatag tactatgaaa tcagttgtca attcacctac
     3601 ttatattaaa agtatattag gaagagtttc agaacttatg ccaaacataa aaggaagaaa
     3661 ttatctttat agagctacaa caccattaga aaaaagatat ataggaaatg caaaagtttt
     3721 tgaaaacagc gaagtaaaaa gattcttttt taagtataag gaaaagaata tatatgaata
     3781 cctgttgtct aatttgtata gagatgcaca aaaaaataat tacgattata taagtaaaat
     3841 gcaacatata gatgtgaata catggcttca aggcgatata cttcaaaaaa ttagcaaact
     3901 ttctacagct gagcaggttg aacttagagt accattttta tataaagatg tatttgatgt
     3961 agcaaaaaat ttaagaatgg agcaaaaaat aaataagaat aatacaaaag ttttacttag
     4021 agaggcgttt agagaaatag tcccagaata tgttgttcaa aggaaaaaat taggatttcc
     4081 aactccaata agagtttggt taaaagattc tctaggggat attgtaaagg aaactattta
     4141 taattcaaat gtagatgaat ttatagataa aaaatatgca ataaaacttt tagatacgca
     4201 tcttaaaggt catagagata attcgagaaa aatatggact atatttacat tttgtctatg
     4261 gcatcaatta tttattgagc ataaaaatgt cgagtattaa tacttttttc aatattaatc
     4321 agagggcaaa agcatgcaat gttttcaccc tcttgattat tatggaatag gataagtaga
     4381 attttgattg attagtttac tatctcaata aaattattgg caaatatttt gacaagatta
     4441 ctttcaacag ctaaatttaa gttttgtcca tcatcatatc cagcggaaat caatcctagt
     4501 aagtttccaa acatatctaa aagagctcct cctgaactac cactagagat tggagctgta
     4561 aattgtatca tttgaaccgt ttcaaaatca cgaaaagctg agataattcc atctgaaact
     4621 gtattaaaaa gacctaaagg gcttccaatt gcaactattt tttgacctct cacaggtttt
     4681 ttactgactt taacaggtat tggtttgcat tttctatcaa cctttatcac agctaaatca
     4741 taatctgaat ggtattttat aatttggtaa gatgtataaa ctttatcatc attttcaaat
     4801 ctaactaaaa atgaataccc atcattgact acatgaagat tcgtaagtat ataaccttca
     4861 ttatttatta ctactccaga accactctta aacggtttat tttcgttatt gcacacaaca
     4921 atcataacaa cagaagctga taactttgca aggtcttcat aagaaagttc tacttcctca
     4981 tcttgtgcaa tttttttaga tgttagattt gtgttattaa gtgtattttt agagttaggt
     5041 ttattcgtag taggagaagt cttattggta ttttcactgt aatttatatt tgtaatatgc
     5101 ctttcaaaag gtatttctaa tatatttgga gttttcctag aaagttcaaa tagagtagaa
     5161 aattttgaac attcaggata taaatcaaga atatctttat ttggatttat atataaaata
     5221 tcacacccaa gttgtgataa aaaatataag aaaaaaagtt cttgcctttt aatatttcca
     5281 aagtatacta tctttggtga aatagtagtc ttcaccttgt tataaaataa tttaggtaga
     5341 aaatagtcca tccaacacaa aagtttaact gcaaagtttt tgatgataga ggatgtacaa
     5401 tttggcatac ttgtggaaaa taaatctaaa gtatttttaa atgcaatctt tttagtccaa
     5461 tttaaagtgt cattttctat tttacatgga aagtcgatat tatataaatc ccaactgtta
     5521 aacttagaac tatcataaat attaaaaatt ttaagcatct tatctgtttg ttctttgtca
     5581 aaatttattt ttagattgtt taatctaata tagggaattg taggattatt aaaaaaataa
     5641 gtatctatat ttttaacttt ttctatgtat ttatcaattg aatcatcaac acctatatat
     5701 cttgtaaagt ggacttcttt aatatatgaa gaactcttat ttttataggt agtatcagat
     5761 ataaattcaa ttagaggttc agttgacttt tttattgaaa tctttaatgg tttaatagta
     5821 caaatcatat tttttctcct ataataagat gatataatct attataaaac aacttaaaac
     5881 aatatagtac aataattttg ta
//
LOCUS       DAELSY010000198         5874 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.199, whole genome shotgun sequence.
ACCESSION   DAELSY010000198 DAELSY010000000
VERSION     DAELSY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.199"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..120
                     /locus_tag="KPG09_003491"
     CDS             <1..120
                     /locus_tag="KPG09_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0968053.1"
                     /translation="DLSIREWICPSCNETHDRDINASINILKEGLRLITTQNK"
     gene            239..757
                     /locus_tag="KPG09_003492"
                     /pseudo
     CDS             239..757
                     /locus_tag="KPG09_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            784..2145
                     /gene="rlmD"
                     /locus_tag="KPG09_003493"
     CDS             784..2145
                     /gene="rlmD"
                     /locus_tag="KPG09_003493"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG0968054.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMNEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            complement(2227..2442)
                     /locus_tag="KPG09_003494"
     CDS             complement(2227..2442)
                     /locus_tag="KPG09_003494"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0968055.1"
                     /translation="MIFDILIITTLEEYTLYFKNPDTMLLENGTNIKDIQHILGHAKT
                     STTMDTYLHVTNNMRNQMVNIFEIIVE"
     gene            2469..2624
                     /locus_tag="KPG09_003495"
     CDS             2469..2624
                     /locus_tag="KPG09_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968056.1"
                     /translation="MFSKNEILKKLIEEYDVKTTTDIQDMLKDLFTNTIHQIFPLSNF
                     NGLNIIY"
     gene            2668..3702
                     /locus_tag="KPG09_003496"
     CDS             2668..3702
                     /locus_tag="KPG09_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBG0968057.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPYEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKNKKRKKI"
     gene            3789..3992
                     /locus_tag="KPG09_003497"
     CDS             3789..3992
                     /locus_tag="KPG09_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBG0968058.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            4163..5323
                     /locus_tag="KPG09_003498"
     CDS             4163..5323
                     /locus_tag="KPG09_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HBG0968059.1"
                     /translation="MKLSKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVIIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
BASE COUNT         2328 a          587 c         1130 g         1829 t
ORIGIN      
        1 gacttaagta taagagaatg gatttgtcct agttgtaatg aaactcacga tagagatata
       61 aatgcaagta taaatatatt aaaagaagga ttaagactaa taacaactca aaataaataa
      121 cttatgtaag aaccgtagga actacgggga tagcctgtgg agaattagta agacatattt
      181 tagtatgcaa aattctgtga agcaggaacc ctgtgacttt agtcatggga ggttcaggat
      241 gaataaacct gatggatata tatcagcaac tacagataaa tatgacccaa cagtattaga
      301 tttaattgat ttatcatact tagcttttga accatttcct gttggaaggt tagataaaga
      361 cacagaggga ttattagttt taactaatga tggaaagctt tctcatagag ttttatctcc
      421 taagaagcat gtgcctaaga cttattatgc aaaaattgat ggagtagtta ctgaagaaga
      481 tgtagaagct tttttagaag gtgtagtgct tgatgacgga tataaaacta tgccatcaca
      541 gttaaatata ttaaaaagtg atgatgaatc agaaatagag cttacaatac atgaaggtaa
      601 gtttcaccaa gtaaaaagaa tgtttgaaag cgtaggcaaa aaagtagtat acttaaaaag
      661 attgtctatg ggcaatttaa aattggatga aagtttagag ttaggtgagt atagagagtt
      721 aacagatgaa gaagtaaaat tgattgaaga aagataataa agatactata gtaaattgga
      781 ggaatgtctg tgaagaaaaa agatgttata gaatttgaag tagataaaat ggaatttggt
      841 ggtacatctt taagtcaagt tggagataga gttatccata tgaagggtgg aataagtggt
      901 caaaaggtga gagctggagt taaaaaagtt agaagtaaga aagctgaagt taagatgata
      961 gaattattag agaactcacc acttgaaact gaagaaactt gcaagcactt tagagagtgt
     1021 ggaggatgta cactattgtc tgtgccatat gaaaaacaat tagaaataaa agaaaaacaa
     1081 gttatggatt tatttttaaa gcaagattta tttggatttc agtttttagg gatagaagga
     1141 agtccagaaa acaaatatta tagaaataag atggaatata cttttggaga tgaagtaaaa
     1201 aatggacctt tgacattagg tcttcataaa aaaggaaagc atatagatat acaaactgtt
     1261 gaggagtgta tgttggttga tgaagacttc tcaaagatat tagtttctag tgtagagttt
     1321 ttcaatgaaa aaaagcttcc ttattataga acaatgaatc acaaaggata tttgagacat
     1381 cttgtagtta gaaaagggat tcatactaat gagattatgg ttaatatagt aacttcttct
     1441 caagaagatt ttgatatgaa tgagtttaaa gatatgcttt tagggattga tttaaaggct
     1501 gagttagtag gtgtacttca tactattaat gatggtttag cagatgcagt ccaatgtgat
     1561 gagttaagag tattgtatgg aagagattat atacaagaag aaattttagg tcttaagttt
     1621 aagatttcac cattttcgtt tttccaaacc aatactaagg gagctgaggt tctttatagt
     1681 attgctaggg attttattgg agattacaat gataaggttg tatttgactt gtatagtggt
     1741 actgggtcta ttggacaggt tatggctggt gctgctaaga aagtgtatgg tattgagata
     1801 gttgaggaag ctgttgttgc tgctaatgag aatgctaagt tgaatggact tactaattgt
     1861 gagtttattg ctggggatgt ggctaaggtt gttaaggatt taaaggacaa gcctgatttg
     1921 attattgttg accctcctag accaggcatt cataaggatg ctattaggga tatttgtgga
     1981 tttggtgcta aggagattgt ttatatttct tgtaatccta agtctttggt tgttgatttg
     2041 gttgatttta aggggtatgg ttatgagatt aagatggtta agtgtatgga tatgttccca
     2101 aatacgccac attgtgagac tgtagttaaa cttattagag aataacaggg tttcagactt
     2161 ttatattgtg tcttaaaagt agatttgcca cctatttgcc accataatta tttttgggtg
     2221 gcaaatttat tctacaatga tttcaaaaat atttaccatc tggtttctca tgttattagt
     2281 tacatgtaaa tatgtatcca tagttgttga agttttagca tgtccgagta tatgctggat
     2341 atcttttata ttagtaccat tttctaataa catagtatca gggtttttaa aataaagtgt
     2401 gtattcttct agagtagtga taattaaaat atcaaatatc actatacaaa ggaaagtgca
     2461 ccctttttat gtttagcaaa aacgaaatat taaaaaaact tattgaagaa tatgatgtca
     2521 aaacaactac agatattcaa gatatgctaa aagatttatt tacaaataca attcatcaaa
     2581 tatttccatt gtcgaatttt aacggtttaa acataatata ttgactgata taataaaatc
     2641 atagaatatt ttagggggaa atagattatg tttaagaaaa agttaatagc tgttgcaatg
     2701 agtataacta tgattagtgt agggagcttt tcgtatgctc attcaggaag aactgattca
     2761 tcaggaggtc atcgtgataa taaaaataaa tctggattag gaagttatca ctatcattgt
     2821 ggtggttatc cagcccattt acataataat ggtggttgtc cttatactgg aggaggaagc
     2881 agttctggaa caacaacttc ggttaataat gaagaaaagc aaaagaggtc tgttggagaa
     2941 aaaggatata atcaagggta tgaagatggg tataaaggaa attatagttc atcaaattat
     3001 agtggagatt atagtgatac atatgaatca aagtactcag aaggatatga aaaaggtaag
     3061 gctaaattag aagaggaaga aaaggtggct aaagagactg gatataattt agggcttaca
     3121 ggtgctaaat ctaataatac atatgaaaaa gaagttttaa aaaatgctta tgataccgga
     3181 tattcaactg gatacaatga atataagact aaaaagatag aagaatataa agctaaaggg
     3241 atagaggata gcaataaaga taaagaaaaa atgacatttg aagaaaacat agattctgaa
     3301 tttatagatg cttataataa tgcttatgac gaaatacaag aacaattaaa aaatgattac
     3361 actactcaag gatttgagag tgctatcaaa ggagaaagat ttgacacatc aactataggt
     3421 aatgttaaat atgctaattg gtttaaagaa ggatatgata atggtaaggt taagttacct
     3481 aaagcaaaag aaagtgtata taatcaaggt tacaatgaag aagattttag tataccatat
     3541 gaaatgaagt ctattgaaac taagttaaaa ggtgtatatg atgaaggttt agaaaaaaga
     3601 gaagaagaga aaagtagaaa tgctacttat ggggttggag ctggaacagc agctgtagtt
     3661 gctggagtag tatataaaaa taaaaaacgt aaaaagatat agatgctaga tataccaatg
     3721 atgacaagtg tataacaaat ataattttaa gtataaatat ttaaaatata gaagggaagg
     3781 ttatatctat gggatttaac tttaggaaaa gcataaatct aggaggagga ctcaaattaa
     3841 atttaggtaa gaagagtgta ggtataagtg ctggtgttaa aggtgctaga gtgtcagtta
     3901 attcaaaagg aagaaaatca gctacattga gtatacctgg aactggaata tcatatacaa
     3961 aaactagtac aaagaaacgt aaacctaaat aaatatttga tatactaaga aatagactgt
     4021 tacaaatagt ctatttttta gtatatagat ttaaaaagtg acaaaatttt gaaatataaa
     4081 ctaatttcat aaaggaaaat attcttttat gtagaattat aatatttaag taatattata
     4141 aaatttatag gaggatttca ttatgaaatt aagcaaaaca ctattggcaa tagggatgtc
     4201 tgtttcatta ttgattgcta gttgtccaac atcaaacgct cttagttcta ttaattcaat
     4261 aaaaggtaca aataagtatg aaacagcagg tttaatagca gataaacaaa attatgatat
     4321 tgcaatactt attaattctg aaaatggatt atctgatgga ttgtcagcaa gtggattgtc
     4381 aggagctaaa aatgccccaa tattgttaac taagaaagat acaatccctt cagaaacatt
     4441 aaaaagatta gaaaaggtaa aagaagtata tctaattgga ggaaaaaatt cgataggagc
     4501 atcagttcag aaaatattaa gtgataaaaa aataagcata aatagaataa gtggaactga
     4561 tagaatagat acaagtcata aagttgcaaa agaaatagga agagtaagta cggttaaaaa
     4621 agtaatatta actaatgctt ttaaaggcga accagatgct atgagtgttg cttctatagc
     4681 agttagagat aaatctccaa tagtattaac tgatggaaat actacaagtt ttgctacaga
     4741 tggattagaa acttatgcaa ttggtggaaa agtcagtatg agtgataagt taataaatga
     4801 aactaaagca acaagaatag gtggagtaga tagatacgat acaaataaac aaataataaa
     4861 taaattttat aatggctcaa aagaatttta catagcaagt gggactgatt tagtttattc
     4921 tttagttggt tctacaattg cacaaaaaac acctatagtg ttagttggaa atggaagtaa
     4981 taagagtata ttaaaaaatg caagtaaaat aactgctatt ggaaatattg atgatgttat
     5041 tataaaacaa tgtttaaata taactaataa tgttggggat tcaaatacag gtattgtaaa
     5101 agatactaat tcaaataata caaacaaacc aagtaatgag cctagctcta ataatcctgg
     5161 attaactgat aaaagtacag tatatgtaac tcctaatgga aaatcatatc atctaacaaa
     5221 aaattgttct acactaaaaa gaagtaaaac aataaataaa gtaacattaa aacaagctaa
     5281 gtcacaaggt aaaatagacc cttgtaatgt ttgtgtaaaa taatgtatta gccaaatata
     5341 tattaaaaaa gacaattaat taagattgtc ttttttattg aagaaatata aatttatatt
     5401 atgtattatt aaaaagaaaa ttattaatta taaaaaataa taataaagac gataagtatt
     5461 aaaattttat gtttccaata tttatgatat aattaatatg gcaaggaata aatatttgaa
     5521 aaagctatga gtggtgtttc catggaagtt tcttcacttt cttatgagag gaggtgaaaa
     5581 ctatggaaaa tctaatgatg agtattataa ctggtgttat agccagttac atttacgata
     5641 aattaaagag ccactctgac caatgaacta tacccactgt tttatacagt taaaacatgt
     5701 aaaagtggtt ataaagtact agtgtcaatc attagtactt tttttattat attcatataa
     5761 ttaatagcta tattttaaag tttactatct aaaaaatagc acgtcaaata aaaccctaga
     5821 tatttaaatt tttttagagc tacttaaaat aaattatgct atctctgtac ttaa
//
LOCUS       DAELSY010000199         5872 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.200, whole genome shotgun sequence.
ACCESSION   DAELSY010000199 DAELSY010000000
VERSION     DAELSY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.200"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            120..1475
                     /locus_tag="KPG09_003499"
     CDS             120..1475
                     /locus_tag="KPG09_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0968060.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATVLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(1593..2261)
                     /locus_tag="KPG09_003500"
     CDS             complement(1593..2261)
                     /locus_tag="KPG09_003500"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBG0968061.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(2328..2756)
                     /locus_tag="KPG09_003501"
     CDS             complement(2328..2756)
                     /locus_tag="KPG09_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG0968062.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            2863..3456
                     /locus_tag="KPG09_003502"
     CDS             2863..3456
                     /locus_tag="KPG09_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBG0968063.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENATKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            3576..4511
                     /locus_tag="KPG09_003503"
     CDS             3576..4511
                     /locus_tag="KPG09_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBG0968064.1"
                     /translation="MDKFKYAFDDKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(4782..5726)
                     /locus_tag="KPG09_003504"
     CDS             complement(4782..5726)
                     /locus_tag="KPG09_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG0968065.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKVCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
BASE COUNT         2037 a          873 c          746 g         2216 t
ORIGIN      
        1 ttatatacta tttgctgttg acattggtat tataccatag tttatcattt atattagaaa
       61 taaaattatt aattaatagt ttcgtaattt tataatagat ttacagggag tgataataaa
      121 tggatacaaa aatatctata aacgaaaaca tgagtctagg taaaagattt tttaaatacc
      181 tagcaccatc tgtagttgca atgtgggttt tctcactata tacaatggtt gatggaatat
      241 ttgtaagtaa aggagttgga gaattggctt tagctgctgt caatatatca atgcctttta
      301 ttaattttat atttgcagtt tctctattat tttcaactgg tgcatctacc ataattgcaa
      361 tctatctggg gaaaaaagat attaaatctg ctaacgaagt cttttcattt aacttagtat
      421 caataattat attatcaata attatattag ctataacttt ttttaattta gatagattag
      481 cactattttt aggagctaca gaatctacaa ttggtatggt taaagattat ttaggaataa
      541 taatattttt taacggattc tttatagtgt cttattcttt agaagttata ataaaaactg
      601 atggctttcc aatattagct actgttggtg taataatatc tgctcttaca aacatcatac
      661 ttgattactt gtttgtaatt gaatttggat ggggtgtaaa aggtgctggt attgcaactg
      721 ttttgtctca agtcttttct actattttct tcttaataca ttttttaaga aaaaactcta
      781 ctttgaattt ttcaaaattt agaattgatt ttaagacact tagaaaaatt gtatttatag
      841 gttttcctga ttcaactact gaattaagtt gtggtatagt tgttttactt tttaacttaa
      901 gtcttactaa atatattgga gaaaatgctc ttatttatta tagtgtcata aattatataa
      961 atacattagt acttatgact atgatgggta taactcaagg tatgcaacca cttactagct
     1021 tttattatgg tgcaggaaat atagataatg taaaaaaatt attaaaaatg gggataaaag
     1081 ccacaataat agcatcagtt gctgtatttg caatttgtat ggctttttca ggaccaatag
     1141 tctctttatt tatacaccca gaagaaacaa tgctattcaa tgaaggagtt cgtgtattta
     1201 aaatattctc tatatctttc ttattggtag gaattaatgt aataatttct ggattctttg
     1261 tatcagtaga aaaaccatca atttctactg ttatatctct tggtagaggg cttgtaatag
     1321 ttgttttaag tctaatttca atgatactaa tatttggtgg acaaggaata tggatgacta
     1381 ctattgtttc tgagtttata tgtcttattt tatcattagt ttttctaaaa aagaattttt
     1441 ctacattaga ctctaatcta aataaggttg cttagatgat aagagtagca atatatgaaa
     1501 atatctaaat aaaaaatacc tattatacaa tcttgcaaat attagacctt tgcaaaaatt
     1561 aggattatat aataggtatt ttatttatgt aattatttta tttctaaata tccatagcca
     1621 tcttttatta aagttccttt tacttcatat tctacaggtt ttttaaatat tggaccatct
     1681 acgacaaatg tatgatactc tccatgttct ccacatgggt ctgcaccagc attttttatc
     1741 ttctccacaa gttctctagt taattttttt cctaaaaatt cctctcctaa gtgctttaag
     1801 tttacttttg ttacaacact acaaaatcca ctgtctataa attcatatac taaatcctct
     1861 ctactctctt gccataatgg taaatctact ttcataccaa ctgcttcaca tctatcagta
     1921 tcccattttc tatgactttc tatatctata tctccatatg cacatatcgt tgctcccatg
     1981 tttttagctt ttattaaagc cctctcaaag ctctcttcat atccttctac accacactca
     2041 gcaactaata aaggtaatcc tatattgcta cttacttgct taagcatatt atattctaaa
     2101 ttatgtgtcc atgactttcc atgttcttca cttatagttg tcaagagtgc cacaggctta
     2161 tatccttttt tcaacattcg atttaatgct aatatactat ccttaccacc actaaatgac
     2221 attacaaatt tttcatcagc caattttaac acctctttca tctaactttg ttttattaac
     2281 ataattatac caaatttttg agttgcatag caaattatat taaattgtta attcactata
     2341 aagaatgtcc ctaatattaa aagtataacc cctatgtatt tgtataaatg cattgtttct
     2401 tgtcccaaaa ttacgctatc aataaaaaat ccaactaaaa cttgggatac aagtgttaat
     2461 ataagtgtat ttgatactcc taaatttgga attgccttta ctgtaaaata tattattaaa
     2521 cctccaaata ctcctcctaa taacaactta taattaaaac catttataga tactagacac
     2581 tttaagtcac ctgtaactat tatacttgcc aaaaaaaata ttgctcctac agttaaactt
     2641 ataaaggtgg ccactaatgc tgttgtactt tttcctagtt ctccatttat aaaagcttca
     2701 agagctgtag aaccacctgc taataatgca aatattattg cccataattt tgtcatggac
     2761 aaacctcctt ctatataatt ctattaatag aagtctatga tacaacctat taattaatta
     2821 ctaagtatag tttaattttt atacactagg ctttatttgg atatgaattt ctgttataat
     2881 tgttctatcg attttggagg tgattttatg tacacaaatt ttaatatcga ttttaataac
     2941 tttaataaaa aagaaaatgc tactaaattt atgttaatgg gagtattgct cattatctta
     3001 ggattattat gccttacatt taagacatta ggtataaaat taatttcttg gacttttggt
     3061 attgcacttt tattctttgc atatttaaat ttaaaaaata ttaatgaact aaaaagatat
     3121 gctactaaag aagaaattaa accctctatt aatatccaat gggttctaat aattgcatgt
     3181 atattacttt ttgtatttcc tcaaaaaata caaagcatat tttcattatt tttagggttt
     3241 tatttaatct ttaatcaact agtagcactt gtaaatagta agaataatcc ttattctaaa
     3301 tttacaactt ggaatatagt taagattcta tttggtatat gccttatatt atctccactt
     3361 tttttatcta gatttatagt atcgataatg tcttttttca taatattatt tggactagtt
     3421 ctatttttct ctggaaatac agcgagaaaa tattaattat tttctcgctg tatttttata
     3481 ttacttaaaa tagaatttgt ttaagttatc tatttgtggt aatatgtttt ttgtacaagc
     3541 tcagcgtaca attttattag aaataggtga tttttttgga taaatttaaa tatgcttttg
     3601 atgataaaag atatcatact tggaattact atcttagaaa tacttttgga gaaaaagttt
     3661 ttaaggtatc tataaatgca ggatttacct gcccaaacat tgatggaagt cttggttatg
     3721 gcggatgtac ctactgtagc aaagaaggct caggtgattt tgctggaaac cctaaagata
     3781 atcttatcag tcaattttat aatataaaag aaatgatgtt aaaaaaatgg cctcatgcaa
     3841 agtatattgg ctactttcaa gcctatacaa atacgtatgc tcctcttgaa gttttaaaag
     3901 aaaaatatga gactatatta gaacttgaag atgtcatagg tctttcaata tccacaagac
     3961 ctgactgttt gccagatgat gttgtagaat acttatctga actaaataaa agaaccaatt
     4021 tatgggttga acttggactt caaacaattc atgattctac atctaaaatt ataaatagag
     4081 gtcatgatta taaaacattt gttgaaggtg ttgaaaaatt aaagtctaaa aatataaagg
     4141 ttgtcgtaca cattataaat ggtcttcctg gtgaagatta taatatgatg atggaaacag
     4201 caaaggcagt tggtaaaatg ggtgtagacg gtataaaaat ccatctattg catgttataa
     4261 aagatactcc tatggaaaag atgttacaaa atggtatgtt aactcttatg gaacaagatg
     4321 aatatatcaa tcttgtctgt gaccaattag aaatacttcc tgaaactatg ataatccata
     4381 gactcactgg tgatggtaag agggatgaac tagtaggtcc tatatggagc ctaaaaaaat
     4441 gggaaatttt aaatcaaatt gatgatactt taaaggctag aaattcttat cagggctgta
     4501 agtttgttta atacaaaata ataactatag aaatggttat ataaatatag tcctttctat
     4561 agttatttag tatttacaaa acaaaatatt aatttgtaca tttatacaat atataataaa
     4621 aaatagatag tctaaactgt atctacttat attttatttg tactgtccac tttattctat
     4681 aaaatttata aatagttatt ttttaatacc aaatataatt tggtataatt tttgttggat
     4741 tctaaaatgt ataccaataa atccttttta agtatataat tctaaatact ttcagttttt
     4801 tcaatctgct ttaacttgtt ggatttataa aatattctta tttcatcata agaaagatta
     4861 agtgctacta ctgcagatat taacatcaac atcactccaa ataacttccc taaggaaaaa
     4921 ctttctccta atatagtgca accaattatt actgaaccaa ctaactctac tgatgccaca
     4981 acacctgcaa ctgacaactc tacaccttta gatatacctg ctgcataaaa aatatatgca
     5041 agtgcagctg gaacaatgcc taacattagc atacaagata aaacatctaa atccttagca
     5101 taatttaaga tttcccaagg tctcgaagaa ggaatcataa atatagctcc aaataagaaa
     5161 ctgtatacta atattgttga acttgaaaat tcctttaaag catttttact aatagttggc
     5221 ataagtgcat atgctattgc tgataatacc ccaagtaaaa gtccaaaagc gtttaaacct
     5281 tgaaaatcca aacgtccacc agtcactgcc ataattgcac caataaaaca aagaataagt
     5341 gaaaataatt tgcttctagt aatattttct ttataacata cttttgaaaa tattgctaaa
     5401 aacagtggtg atgtatatag taaaactgct gcaattgata cacctgcaat gtcaattgct
     5461 ttaaaataac ataaattaaa cattgcctga catataattc ctattattac tgaatatata
     5521 attccttttt tgcttatctt taagatttgt ggtgtcctta tagatgaata tacgcctaaa
     5581 actataaatc caaaaaacaa tcttgtaaat gctacttgtt ctgatgtcag tccatttccc
     5641 attaaaaact ttccaaataa tccaagtgta gcccataata accctgctat tgctataaat
     5701 acataccctt tacttttttc tcccatttta aattcccttc tatttttatt tagtcaattt
     5761 acactcttct ttttaagcaa aaattatgcc atcttaaacc aatatatatc gactctaaaa
     5821 ccctagtctt tccaataata ctaataagtt ttcaattttc ttatatttat tt
//
LOCUS       DAELSY010000200         5745 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.201, whole genome shotgun sequence.
ACCESSION   DAELSY010000200 DAELSY010000000
VERSION     DAELSY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.201"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            88..2319
                     /gene="pflB"
                     /locus_tag="KPG09_003505"
     CDS             88..2319
                     /gene="pflB"
                     /locus_tag="KPG09_003505"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBG0968066.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFS
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            2479..3213
                     /gene="pflA"
                     /locus_tag="KPG09_003506"
     CDS             2479..3213
                     /gene="pflA"
                     /locus_tag="KPG09_003506"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBG0968067.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3335..5005)
                     /locus_tag="KPG09_003507"
     CDS             complement(3335..5005)
                     /locus_tag="KPG09_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HBG0968068.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYEEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
BASE COUNT         2027 a          825 c          828 g         2065 t
ORIGIN      
        1 ttaaaaaaat atataaaata aattaagtat ataaaaaata attttaatag tattatttat
       61 catttaaatc taaggaggaa atacttaatg aatgcatggc aaggatttaa aacaggaaga
      121 tggactaaag aaataaatgt tagagagttt atacaattaa actattctcc atatgaggga
      181 aatgactctt tcttagctgg tgccactgaa aatactaaaa aattatggga cgaagcaatg
      241 gtcctattta aaaaagaaag agaaaatggt ggtactttag atgttgatac aaaaactgtt
      301 tctggaatag ctgcatatgc tccaggatat ttagataaag aattagaaac aatagttggg
      361 ttacaaacgg atgccccttt aaaaagagct gtcatgccat atggtggtat aaagatggtt
      421 gaaaattcat gcgaagcttt tggatatgaa ttagacccag aaataaaaga tatatttact
      481 aaatacagaa aaacacacaa ccaaggagtt tttgatgttt atactccaga aatgagagct
      541 gctagaaaat ctggtataat aacaggtctt ccagatgctt atggtagagg tagaataatt
      601 ggtgactaca gaagagttgc tctatacggt gttgatgctt taatagaaga taaaaatgag
      661 caaaagaaat cattagaagt ttcttgcatg gatgaggaag ttatcagatt aagagaagaa
      721 ataactgagc aaatatcagc tttaaatgaa cttaaaaaaa tggctgaatc ttatggattt
      781 gatatatcaa aaccagcaac taactcaaaa gaagctgtac aatggttata ctttggttat
      841 ttaggtgctg ttaaagacca aaatggtgct gcaatgtctt taggtagaac atctactttc
      901 ttagatatat actttgaaag agatttaaaa gctggaatcg taacagaaga agaattacaa
      961 gagtatatgg accattttgt tatgaaatta agaatggtta agttcctaag aactcctgat
     1021 tacaataacc tattctctgg agacccaact tgggtaactg agtgtatagg aggtatggga
     1081 atagatggta gaacattagt tactaaaaac tcatttagaa tgttaaatac tctatataca
     1141 ttaggacctt ctccagagcc aaacttaaca gttctatggt caactaaatt acctcaagga
     1201 ttcaaagatt tctgttctaa agtatctata gatacaagtt cagtacagta tgaaaatgat
     1261 gatttaatga gagcatactg gggagatgac tatggtatag cttgttgtgt atctgcaatg
     1321 agaattggta aacaaatgca gttctttgga gctagagtta acttagctaa aactttatta
     1381 tacgctataa atggtggtgt agatgagaaa tctggtgttc aagttggacc aagatttgaa
     1441 cctataactt ctgagtactt agattatgat gaagttatga gtaaatttga accattcact
     1501 gattggttag caacattata tgtaaatact ttaaatgtaa tacattatat gcatgataaa
     1561 tattcttatg aagcattaga aatggcatta catgatagag atatatttag aactatggct
     1621 tgtggtatgg ctgggttatc agtttgtgca gactcattat ctgctataaa acatgctaag
     1681 gttaaaacta ttagaaatga acaaggaata gctgttgatt ttgaaataga aggagattat
     1741 ccaaaatacg gaaacaatga tgatagagtt gatagtatag ctgtagagtt agttgaaagc
     1801 tttatgaata aaataagaaa aaataaaact tatagaaatt cttatccaac tcaatctata
     1861 cttacaataa cttcaaatgt tgtttatggt aagaaaactg gtaatacacc agatggtaga
     1921 agagctggtg ctccatttgc tcctggtgct aacccaatgc atggtagaga tactaatggt
     1981 gctttagctt cattatcttc agttgctaaa ttaccatatg aacatgctca agatggtata
     2041 tctaatactt tctctatagt acctgctgct ttaggaaaag atatgactga aagaataaac
     2101 aatctttctg caatgatgga tggatacttc tctcaaaatg ctcatcactt aaatgtaaat
     2161 gtatttgata gagctacttt agaagatgct atggaacatc cagaagaata tccacaatta
     2221 actataagag tatctggata tgcagttaac ttcataaaat taactaaaga gcaacaatta
     2281 gatgttataa acagaacatt ccacggtaaa atggcttagt ctttaaattt ttatacttta
     2341 actaagatta ctactgtgta aaaaataaaa ataaaaggct atcttatatt tgaattattt
     2401 ctggtataag atggcctttt ttagacaaat attataacta ttgataaggt tattaaaata
     2461 tattgtgagg tgaactctat gattaaggga aagatacatt ctatagaaac gtttggtact
     2521 gtagatggac caggaataag atatatatta ttttttcagg ggtgtcctct tagatgcaaa
     2581 tactgtcata acagggatac atgggatatt aaatcaggaa aagaatacac agttgatgaa
     2641 ataataacgg atgctcttaa gtatacttct tttatgaagt tttctggtgg aggtataact
     2701 gcctctggtg gtgaatccac tcttcaacca gaatttttaa gtgaattatt taaaaaagca
     2761 aaagaaaatg atatacacac ttgcttggat acatctggat tcgtagatat agaaactata
     2821 gacccagttt tagataatac tgatttggtt ttacttgatt taaaacatat ggttgaggaa
     2881 aaatctattg atttgactgg tgttggtatg gataaggcat taaaacttgc tagacactta
     2941 gaatcaagaa atattcctgt atggataaga catgttttag ttcctggcat tactgatgat
     3001 atagataatt tagaaaaact aggtcaattt gtagctacat tgaaaaatgt agaaagattt
     3061 gaattgctac cataccattc tatgggaata cacaaatggg aaagtttagg tatcgattat
     3121 gaactaaaag atgttcctga tgccactaag gaagatgtcc aaaaagctag tgaaataatc
     3181 tctaaatttg gagtgaaagt atataatagt tagtgtagta tattaatcct ttatagaaga
     3241 cataacaaaa tagggtaaag tgtaaatttt taataaactt caaaacttcc actttgccct
     3301 atttttatat actatatatc tcattttatg tacatcactt tgatagttga aatttataaa
     3361 attcagaggc acttacaggt cttccaaata aaaaaccctg aataatatct acacctaagc
     3421 tttttactat atcatactct tcataagact ctattccctc aacacaaact tttaaattta
     3481 taatatgagc taatttaata atatattcaa gaaaagtata ttcataacta ttatatgtaa
     3541 tattcttaac aaaacttctt tcaattttta ttatattaac agacagttct ttaaggtaat
     3601 ttaaagatga ataccctgta ccaaagtcat ctattgcaat atatactcct atacccttta
     3661 aactaataaa tttatcattt agtaaattta tatctggtat ccaacaattc tctgttaatt
     3721 ccaaaattaa aaattcaggt cttaattgat actctactaa acattctact ataaaatctc
     3781 taaaaaaatc ttcttttaac tgaatatacg atacatttac agatatttta aagtctggat
     3841 ttattttatg ccattcttta cactgtttta cagcttcttt tataatccac ttcccaactg
     3901 gtataattaa attactctgt tctagtattg gtataaattc aacaggagat acttctccat
     3961 aagtactcga atgccatctt aaaagtacct ctgcccctat tacctctttt gtaacagcat
     4021 ttacctgggg ttgaaaaaac agctcaaagt cattaaaatt attttcaacg cactctctta
     4081 gtttttgttg cattgaaatt acctttagtt tattttcata gagctcttgt gaaaaaaact
     4141 taattctatt tttaccacta atttttgcta tatccaatgc aatatcagca tgtttaaata
     4201 aatctaaata attatctcca tcttctggat acatagcaac tcctgcagtc actgtgcaat
     4261 agtacttatt tgattcaatt tcatgctgag tatttgtata catctgtatc ttttcatata
     4321 ttttctctat agtttcttta gtacacatag ggtaaaaaaa tgcaaattca tctccatcta
     4381 acctatacaa tcttacatct tttggaagat attctaatac ttcagtagca attgcacgca
     4441 atactttgtc accataagaa tgtccatatt tttcatttat atttttaaaa ttatcaatat
     4501 ccatgataaa catagcacca tttttataaa ttttctcttt tataagataa ttcattctat
     4561 gttcaaattg ttctctattc cacgttcctg tgatagaatc aaatttattt ttttcaccaa
     4621 tattctttat tctcccgact agaaatatag tttttgggtc atccgacaca taaactttac
     4681 ctctacaaga aatccatact atttcttcat atttattaat caacctatat tctaaattgt
     4741 gctcaccttt ttttccatgt agtaattctt gtatatcatc tttccatatt tgaacatcat
     4801 ccggataaac aattttactc caacaattga ctaaatcaga ttctatctct ttacttaagt
     4861 taaattcatc aaaaattgct gaagaaattt taaatttatt cctatttata tcccaaaaaa
     4921 atatataatc ttctgtactt tgagataaaa gttcaaagaa aatttctata tggtctaaac
     4981 ttaaagagtt cgattcttct ctcatctttt ccccctacaa tattactatt agtgtagttt
     5041 aatcaacaaa atattcttat ttagattata ctattttact aattttttta tctcatcaaa
     5101 cacatttccc caactttagt tatcttatca aatctcttac tcttaactaa gtttttttga
     5161 gattcataaa gtaatttttc tacaatcatt actggtgatt tattttcacc tgctaaaaca
     5221 ccatgttaaa tcatttatta aatcattttc tcttaaaagt ccatttttta ttatataaag
     5281 agttctcagt tctttattct ataaagcttt tctattactc ttgaatttgt tttatttttt
     5341 ctcagcctat tctctttgga tttggtattg cttttctata tattttggca aagaagtgcc
     5401 tcttcctata atataagatt ttgatatttc ttcatcatct ataaattttt taatagggat
     5461 tatatcattt tttatattta tttatataaa tattttttgc ttttaatttt ttatctctta
     5521 ttatgtactt acatttaatt tattcttttc agttaataaa ataggtacat ttttagtaca
     5581 tgaaaataga gttgcttaca gggtatctag gattgatgta ctatttacta gtattacatt
     5641 ttcagccgat tcaacccctc ttttgcttac ataatttaag tatatttttc tggattttta
     5701 aatttatgta ctttaaaatg gatatcctta ttttcattga tttta
//
LOCUS       DAELSY010000201         5709 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.202, whole genome shotgun sequence.
ACCESSION   DAELSY010000201 DAELSY010000000
VERSION     DAELSY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.202"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            161..661
                     /locus_tag="KPG09_003508"
     CDS             161..661
                     /locus_tag="KPG09_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBG0968069.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIISGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            718..1704
                     /locus_tag="KPG09_003509"
     CDS             718..1704
                     /locus_tag="KPG09_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG0968070.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            1754..2476
                     /locus_tag="KPG09_003510"
     CDS             1754..2476
                     /locus_tag="KPG09_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBG0968071.1"
                     /translation="MENFNKNAKIIGIGVEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            2505..4439
                     /locus_tag="KPG09_003511"
     CDS             2505..4439
                     /locus_tag="KPG09_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0968072.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFIEESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALDGGYFFPVRWIEYGKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNKYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            4432..5700
                     /locus_tag="KPG09_003512"
     CDS             4432..5700
                     /locus_tag="KPG09_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HBG0968073.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKIRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDECSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLGYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDDIKNTKNDLKKIIFNYKF"
BASE COUNT         2395 a          430 c          974 g         1910 t
ORIGIN      
        1 atttgtatgt attatattgt ataatataaa atgaatttgg aaaatagttc gctaaaataa
       61 tttgttttta taaaattact gtttaagttt atagattttg tataaaataa agttatgtta
      121 ttaatttttt tagaaataaa acttttacta ggagacagat atgagaaaaa atagtattaa
      181 tatagcacta actgcagcaa ttacagcaat ctatgcagtt ttaacattat cattgggatt
      241 tataagttat ggaccaatac aatttagggt agcagagatt atgatgcttt tggcattttt
      301 agataaagga tatatagtag gtcttacatt aggttgtttt ttagcaaatg taataggacc
      361 atatggtgta cctgatataa tttctggaac attagccaca tttataagtg caagtatgat
      421 ttatattact agaaagttag gtaaacaaaa taaaagtact ttagttatag cttctttatg
      481 gccaactgtt ataaattcga ttgtggttgg tctaatgtta aatgtattct ttgggcttcc
      541 attaattcta tctatgatac aggtaggttt tggagaattt gtagtagtaa caattgtagg
      601 agtaccattt ttctgctttt taatgaaaaa atataaaaat gtcattaata ttaaatttta
      661 aatagtgtat agtaatttaa ttaattgaga gtattattag tgaaaagagg tgtttatttg
      721 tcgaaggtaa aaagattgac tgatgtttgg atatatatta atgataagca aaagtttaca
      781 ataaaagagt tgtcagagga atttaatctt tcagctaaaa caatacaacg atatttaaaa
      841 gaattaaata aaatgggact tccaattcaa gcagaacaag gtagaaatgg aggttatagg
      901 gtgctaaata atagctatat acctcctgtt atatttacag aaaaagaagt tatgattata
      961 ctatttgcac ttaagtcact tcagatttat aattataagt taatggaaat agaaattaat
     1021 tctataatga gaaaagtaag ttatgaacgt aaaagcagta taaaagtagg tatagaaaat
     1081 attaaaagat acattggctt tgtagctaat gatactaatg atggagattt taagagtagt
     1141 aatgtaactg agttttttag agcttcttca gaatctctaa ttttaaatat taagtataag
     1201 tgtggtaatc agattttgga aaaaaacgta tgccctatag gtatatattt aaataaggga
     1261 gcatggtttt caccagcata tgattgtaaa agtgataaaa taatattagt tcatatagat
     1321 aatataattg atataaaaag aacaggagaa agtaaaaaaa tacatataaa cttggaagaa
     1381 tggttacatg aagtatataa caatttatat ggaaatagtt caactactaa aaaatttggt
     1441 gaaattattt atctaaatgt attattaact aaagaaggtg tttctaaaat aaaggacagt
     1501 tcttggaatt tagatatgga gctaaatgaa gatggtagtg gaataattag tacattcata
     1561 aaggttgaag atatagattg ttatgcttcg attttatttt ttattggaaa taatgctaag
     1621 gttatagagc caaaattttt gaataagcta ctttatgata aggccaatga gttaagatat
     1681 ttttatgaag aaaatatggt ataatattta gaaatgttga aattaaataa ttattaagga
     1741 gatatacgtt ataatggaaa attttaataa gaatgctaaa ataattggta ttggagttga
     1801 aggtattaat ataataaatg aagtagaaga aaaaataaaa gcaaatatgg atatagaaaa
     1861 aataaacata aatcaagaga ttgaaaaaga atatgttaga agtcttttag atggtgtaga
     1921 tatattattt ttgatatatt catctgaaga taaacatatt agagatatta taaaagctgt
     1981 ttcatatatg tcaaacgaaa gaagagttct atctatagga atggattgtt cagagaaaga
     2041 aaataaagaa gatttagagc ttgggagaga gtttaaaatc aataatgata gtatatttaa
     2101 atttgttgat ttaatgaata tcatggtaga gtctatttct gattcttgca tgataaatat
     2161 agatattact gatttaaaag aagctattgt aggagataaa ggaattaagt attcttttga
     2221 agagtttgaa gatacaaagt catatagcga gatagctgac attttatttg acaggatgga
     2281 atatattggt gaggaattta taagtaagaa aggaattgta tttgttgaag gaagtccaga
     2341 attttctata atggaattga atgatttaat aagtaatatt cagagcaagg tagaagaaag
     2401 ctatgaagta atattttctt tatatataaa ggaaaattta aatggaaata ttagagtagg
     2461 cttattgtat aattagtata ttttagatat taaggagagt gtatatgtct gaaaatataa
     2521 gaggtacaga gtattggtta atggaagaat ttttttatca ggaaacagac acaccacaga
     2581 tttttataga agaaagcata attgcacttg gatttgatat aaaaataaat ttaaaaaaag
     2641 tattcagtat ggggaaaaaa tcagaaaagt atattaatga gaattgtaat gataaagatt
     2701 ttataaaaaa gtttatgtcg attaaaaaag gagattattt tttatataaa gcatatcaaa
     2761 atatagaaga aaaagttaaa tgtaatgtgt ctattggcat agttgaagaa gattttgaaa
     2821 atggatatga attaagtcat attttagggc atacactacc agtaaaatgg atgcataatt
     2881 ttgatacagt ttcaaattgt aaagtatata caagtgaatt ttataaatta aataataatg
     2941 atattagttc atatttagaa attattaagt cacaagataa gaaattattg agaaaaagaa
     3001 tagaagaaag tgaaaataat aaatggtatt taaaaaacta ttattataaa aatgtagata
     3061 agtgtgtaga tgatgaagaa ttatttattc agcccattaa tctagaacaa ttagaaagcc
     3121 taaaaaatat aaagacagat gattatattt tattgtataa tagcgagaaa gtaatttgtg
     3181 aaaatgacca tacaatgaag agtctatttg gcgtatgtca agttatggaa gggttcgata
     3241 aagagaaagt aagtgctttg gatggtggat atttttttcc tgttagatgg atagagtatg
     3301 gaaaggagaa tatagacgga aatgcatggg ttatatttga aaataatagt atagttgaaa
     3361 agtatgtaaa aagatttact aataaaatgc cattaaatac tatactttat ggaccaccag
     3421 gaactggtaa aacatacaat acaaaacaga tggtttacaa tctagtaagt gaaaccagtc
     3481 taagaaacaa atactataca ggttacttag attataaaaa caatgtagaa ttttgtacat
     3541 ttcatcaatc atatggatat gaggaattta tagagggtct taagtctgat ggtgaaggca
     3601 attttaagcc agaagatggg atattaaagg atatatctat agaagcatgt tataatgcat
     3661 tgaaatttga aaaaaagatg agctttgagt tggaaaaaga tagcttgact ttagaaacta
     3721 taaaagacag gaaaaagaaa ttagtattag aaaatataga atatagtgag agttttaatt
     3781 ttgaagaatc agaaaactat gttttaataa tagatgaaat taatagaggg aatgtatcta
     3841 aaatatttgg tgagctaata acattattgg aagatgataa aagacttact aaatcaaatc
     3901 aaactattgt caaactacca tattcaaagg aaaaattttc tctacctcca aatttgtata
     3961 tagttggaac tatgaacaca tcagataaat caatagcact tttagatata gctttaagaa
     4021 gaaggtttaa ttttgaagaa attatgccag attatgattt attgtctatt gtagatggta
     4081 tagatattag aaaaatgcta tatacaatca ataaaaggat agagttttta tatgatagag
     4141 accatgttat tggacaagct tatctacttg ctgtagaaaa tattaatgat atagtagtag
     4201 tatttagaag taaaataata ccacttcttc aagagtattt ttattatgat tcagaaaaag
     4261 taggtttgat tttaggagga attggaagaa atgaaaaaga taaatacatt gtttataaag
     4321 aaaaacttat agcttctgag ttatttaaaa ataataatgc ctcaataatg gagagtaaag
     4381 ttaactattg tataaaacaa aacatatcta gtgaggagct aaaaaatata tatgagtaaa
     4441 aatatatatg ttaaggaaac ctatgaatgg ataagggtag gtaatggtga aaatgagctt
     4501 acagaaattg aatatgaaaa attgcttaaa tatctagaga acaataatga tgtattaaaa
     4561 agtaatataa tagatattaa atacaaaaaa attagattta taaactatgt aggcatcata
     4621 tgttttgaaa atgtaatact tgaaatactt cctaaattgt cattaagtga taatcttgta
     4681 aaagatagag agatactatt acagatgctt tctatatgta ataaaattcc aattacaatg
     4741 aatgaaaaaa taagattgag tttgaaaaat tataatctct taaatttttt tgttatgtat
     4801 ttcattgaga gtatgcaaac acagatgaaa aaaggtattt attttgaata tataaataaa
     4861 atagagaact taaatgttat gaggggtaaa atattattaa gtacatatgc aaaagaaaaa
     4921 ggcatttctc caatgaaaat aagatgtgaa tatgatgagt gtagtgaaaa taatttcttg
     4981 aatcaagttc ttaaaaaagc atgtatatct attttatgta gaataaatga caattcaatt
     5041 caaggcaaga ttaaaaaaat tttaagctat tttcaaaatg tagatttaat ttatatagat
     5101 agaaaaaaac tgttgggtta taaattttat aaaaacaatg atagatttaa agattgttat
     5161 ttactagcta gacttctact tttaaatcta tctatggata atagtcaaaa taatcaagag
     5221 gcattttcta tattatttga aataaatact ttgtatgaag aatatatagg aattttaatc
     5281 aaaagtatat gggacaattc atttagagaa acatatatac aagataagag taaatttctt
     5341 ttgaaaaatg aacaaacagg taagaaaaac tttaatttaa gaccggatat agttttatat
     5401 gacttaaaaa atgaatatga aattataatt gacactaaat ggaaagctat tgaagtagat
     5461 tcaaatgttt tttataggtc aagtgatata taccagatgt atgcatatat aacagcatat
     5521 gaaaatgcaa aaagatgtat attattatat ccttgtattc aaaaagataa gaattatagt
     5581 tcctggaaat tatcagaatc atttaagggt aagtttatag aagcaaaaac tgtgaggtta
     5641 gatgatataa aaaacacaaa aaatgattta aaaaagataa tttttaatta taaattttaa
     5701 aaataagaa
//
LOCUS       DAELSY010000202         5608 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.203, whole genome shotgun sequence.
ACCESSION   DAELSY010000202 DAELSY010000000
VERSION     DAELSY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.203"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            181..486
                     /locus_tag="KPG09_003513"
     CDS             181..486
                     /locus_tag="KPG09_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968074.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            894..1583
                     /locus_tag="KPG09_003514"
     CDS             894..1583
                     /locus_tag="KPG09_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0968075.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYFVKTAVDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNTIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            1583..2500
                     /locus_tag="KPG09_003515"
     CDS             1583..2500
                     /locus_tag="KPG09_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0968076.1"
                     /translation="MELFLVVISIVLFAFYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATLEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            2573..3499
                     /locus_tag="KPG09_003516"
     CDS             2573..3499
                     /locus_tag="KPG09_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0968077.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNDVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFSKKVAETDFEILKKIGTIIENPYFYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     KNQIGINSIGRKQSSLEDYFFQTTGTGGIETL"
     gene            3496..4176
                     /locus_tag="KPG09_003517"
     CDS             3496..4176
                     /locus_tag="KPG09_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0968078.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSILGILLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLVFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            4621..5322
                     /locus_tag="KPG09_003518"
     CDS             4621..5322
                     /locus_tag="KPG09_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG0968079.1"
                     /translation="MNNSFLDKLIETKELKNSLYNVLKHNFLYHSNKIEGSTFTTEAL
                     ALLLDKNVVTGRHTLDDVQETVNSSYVFDTVIDSLKEKITHNFLRNLHSSLIFNTTLH
                     SRGMAGIYKTIPNMILGTDVSIAQPFEVEPKLDELIEWYYSQSEVSIKVIAEFHYRFE
                     LIHPFQDGNGRIGRFVMLKQMLENNLPIKIVSWDSEDLYRNSLNSCSLGNYVPLIEYL
                     SSLEDFREVYKMLWK"
BASE COUNT         2167 a          582 c          869 g         1990 t
ORIGIN      
        1 aatttataat aatatctata tgaaaattag aattatttta tcacaaaaaa attaagttaa
       61 ataaatatga aattacttcc aaatatactt ccaaaaacat gtaatcgcat atataatata
      121 attataaaaa taataaatat ttttatagta gtagttaatt attaataagg aggggtcaaa
      181 atgcctagca tgattatgga agttgtagga gtactattta tagctattgg attaatcttg
      241 acattcattg aaataaaagg aaatttccct tctttttatg gttttggtca aaattctata
      301 gggaaaaatg ctgtaggtat tatagcagca ggaattgctt taattttctt tagaaatggc
      361 atagatagca tagattctat agctacttat ggtgtatttt tagcaataga acttgttgta
      421 atagttgcat atttcttaat aagaaaaaaa tttactaaag aagataaaca tttaagaact
      481 aattaactaa attatctact ataaaattgg aaaattaaaa acaaataaaa gtttgttttt
      541 aattatttaa aatcccaatt tgattatttt taaagaaact atattctgta taaaactaga
      601 atgtggtttc ttttttatgt ataaaatatt ttttagcaaa agttaaaagg tgttttataa
      661 tataaaatag ttaattgtag atgttaataa aagaaaatta tgtatatttc agataaagat
      721 aagagagtat actatatttt aattattttt tcaataaaac ttattgaaaa gaactttaac
      781 ttagaagagt atcaccaaat tgcagataca atatcataag cagttattta ttgacaaccc
      841 tttttaataa atatatcatt tgaagtatac tgacgaaaag gagttgaata ataatggaca
      901 tcttattggt tgaagatgat aagaatattc gagaaatggt atacgatttt ctgattagtg
      961 agaattattt tgttaaaact gccgttgacg gaaatacagc tattgaaata tttaaaaatt
     1021 attcatttga tattatttta ctggacttga tgttaccaaa gtctagtggt tttgaagtat
     1081 taaaatatat acgaagtgtg agtgttgttc ctgttattat tactactgcg aaagatagtg
     1141 acacagataa aatgctaggg cttaatcttg gtgcagatga ttatattaca aagccttttt
     1201 caatcgtaga attgcttgca agaattaaag ctaatattag acgtgcaacg gtgtatagca
     1261 gttcaagaaa ctctgaagat atggttattc agtataaaga tttgattata aacatatcaa
     1321 aatatgtagt ttcaaagaat ggaattgact taaacttaac acatacagaa tttgaaattg
     1381 ttaaattact agcgactaat caaggacgtg ctttttcaaa ggaacaatta tacaacacta
     1441 tttggaaaga accttattat ggaaatgaaa atgtgctaaa tactcatatt aacaggttaa
     1501 gaaataaatt gtctgatggt atagaaaaaa ataattatat taaaactctt tggggaattg
     1561 gctacaaaat ggaggaagaa taatggagtt atttttagtt gtaatttcga ttgtactatt
     1621 tgctttctac ttaaaagaac gaaataagtc aaagaaaatg gaaagtgatt ataagtatat
     1681 caattcaaga cttcgtgata taacaaatga tgatgaaatt aattatattt tagtaccatc
     1741 tgatatttcg attgtaaaag aaacagcagt agatataaat aaacttcttg aaaagtttta
     1801 cagtaggcaa attaattata atagaagtga aaaagcgata ttgcaaatat ttacaaacat
     1861 atcacacgat ttacgaactc ccatcacagt attaaagggt tatattgaaa tgctatatct
     1921 tcaaagtcaa aaagaagaat tatcttcttc aatgagagca actttggaaa aaatgcaaac
     1981 aaattcaaat gagcttgtta attcaataaa taacctgttt aatatggcaa aaatccaatc
     2041 tggagatatg atattaaatc ttcaaaaatc caatctaaca caattatgtc atgagattat
     2101 tttagagttt tatgatttac tagataaaga aaactttaat gttgaagtta atattgaaga
     2161 tagacctata tatgcaaatg ttgacacaga agcattacaa cgtatactca aaaatttaat
     2221 tgataatgct ataaaatatg gttcagatgg tagattttta gggatttcac tatatgaaaa
     2281 ggaaaaacat gtttttatag atattgagga taaaggttca ggtatttctg aaactgaaaa
     2341 agagcatatt ttcacaagag cttatactgc tgacagaaaa aaaggcaatg gtttaggttt
     2401 ggctatttct caagggcttg caaactctat gggaggttca atttatgtaa atagtagacc
     2461 taatgtaaaa acagttttta cggtaaaatt gaaaagttag aaaaaagtta gatttaaaag
     2521 agagaaaaga taattttatt ttgtatactg tttttagaaa ggagggagta tgatgaacta
     2581 tatcatagag acccatcaat taaagaaaat atataaagat aaagctgttg ttaatgatgt
     2641 taatattcat gtgaaaaagg gagaaattta tggatttgtt ggaccaaatg gagcaggtaa
     2701 aagtacagta atgaaaatgt tgctaaatct cgttaagcca aattctggag aaatagttat
     2761 gttcagtaag aaagtggcag aaactgattt tgaaatatta aaaaagattg gtactattat
     2821 tgaaaatcct tatttctatg agaatttgac tgctaaacag aatcttgatt tacactgtga
     2881 atatatgggt tattataaca aagaacatat atcagaggta ttggaatgtg tcggattgtc
     2941 aaagcaatca aacaagaaag tttcaaaata ttctttaggg atgaaacaac gtcttgcaat
     3001 agctagagca attcttacta aaccagaatt attgattctt gatgaaccta taaatgcact
     3061 cgacccagag gggataagag aaatgcgaga attattccgt aagttaaata cagattatgg
     3121 aataactata tttatttcta gtcatattct ttcagaggta gaacaaattg cggacactat
     3181 tggaattatt caagatggta aacttattaa agaaatttct atgtcagata tacacaaata
     3241 tcaaacagat tatgttgaag tagatgtaga taatgtggaa cttgctggat acttgttaga
     3301 aaaagaattt ggaataaaag actttaaaat tacttcggaa agttgtattg aaatatatga
     3361 tttacgaaaa gatgtaaaag aaatatccaa aatatttatc aaaaatcaaa ttggtataaa
     3421 tagcattgga cgtaaacaaa gttctttaga agattacttc ttccaaacta caggtacagg
     3481 aggtatagaa acactatgat acatttaatg aaactagaat taaaaaaagt tgggctaaaa
     3541 agatattttt tattttcaat actggggatt ttgttatgta tgttttttgt ctttactgga
     3601 ttgaatgata aatctacttc aatatatgat tatgatgtta catttaggac aatcagttta
     3661 gttttctgtt tttactatat tatattattt gctgtgttga atgtggcata tataataaat
     3721 gaatatacaa ataaaacaat cttgatttta ttctcttatc cacttgaccg taaaaaactt
     3781 attttatcaa agttactttt gattacgact cttatgatat catcaatgat agttggatat
     3841 atctgttgtt ttacattcgt tgttgtgttg gataagtatt ttaatttagt tattggagat
     3901 tttcatattt caattttatc ttattggata ccaggagcaa ttaaaagtat tattgtattt
     3961 tgctcattag gtatatggac attcgttgtt ggaatgataa gaaaatcaat acctgttacc
     4021 cttgtcagtt caattatttt tatcaattta agacaattta ttttagctgg tacaaataca
     4081 actcaagaca gtctgatttc tgtttttatt atagttggaa taactgtgat tggtatatat
     4141 tatatactaa atcataaagt tactgaaatt gactagatac tgtggatagg aaggtagtta
     4201 ttaattgtat cttcttggga taataatgat ttgctattat atcagctatt attaatatta
     4261 ttagagtttt aataatatta ataatagcta tatatttttt cttcttattt ataaatttaa
     4321 tgatttatag tcataaaatg aactctaaga agagataaaa atgcgtttta tatgttttta
     4381 gattaatatg aaatatcaca atgaaatgtt gtacaaagtg atatataata attatatgaa
     4441 ctataaaatt gactaaactt attgactaat aaattagatg aaaagttgag acttactgaa
     4501 aataaataca atgaaatata tactacaagt attaaattaa atctgaactt tacatactat
     4561 tagaataagt attataagat tataatgatt taaaaatatt aaaataattg aggtgataac
     4621 atgaacaatt cctttttaga taaattgatt gagacaaaag aacttaaaaa ttcactttat
     4681 aatgtgttaa aacacaattt tctttatcat tctaataaga tagagggcag tacttttact
     4741 actgaagcat tagcactttt actcgataag aatgttgtta caggaagaca tactttagat
     4801 gatgtacaag aaactgttaa ttctagttat gtatttgata cagtaattga ctcactgaaa
     4861 gagaaaataa cacataattt cttaaggaat ctacattcaa gtttaatctt caatacaacc
     4921 ctacattcca gagggatggc tggtatatat aaaactatac ccaatatgat actaggaact
     4981 gatgtaagca tagcacaacc atttgaagtt gagccaaagc ttgatgaatt aattgaatgg
     5041 tactatagtc agagtgaagt gtctattaaa gttatagctg aatttcatta tagatttgaa
     5101 ttgatacatc catttcaaga tggtaatggt cgcataggta gatttgttat gctaaaacag
     5161 atgttagaaa ataatcttcc tataaaaatt gtatcttggg atagtgagga cttgtataga
     5221 aactccttaa attcttgtag tttaggtaat tatgtccctt taattgaata tttatcaagt
     5281 ttagaagatt ttagagaagt atataaaatg ttatggaaat aattgtttgt agcaaaaaaa
     5341 gtttttatat aaatatattt ttaactatgc tatttaacgt tgatttctta gttattctta
     5401 gcttggacta tacccactat tttaactata taaaatagtg gatatagttt aactatctct
     5461 ttataactat ttttatgtat cttagttgtt tcttaatatt gggacagttc ataaaactaa
     5521 aagtgatttt taattttatc atttataatt taccattaca tcagaaaaat gcttatcatt
     5581 agaattttca taaaaggtac ctattttc
//
LOCUS       DAELSY010000203         5456 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.204, whole genome shotgun sequence.
ACCESSION   DAELSY010000203 DAELSY010000000
VERSION     DAELSY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.204"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            18..800
                     /locus_tag="KPG09_003519"
     CDS             18..800
                     /locus_tag="KPG09_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0968080.1"
                     /translation="MSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSNPNKKGNIK
                     SQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGKSMPTDEPI
                     SFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEKIKLIDDYD
                     AVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDVTPKISTVY
                     ADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            826..2745
                     /locus_tag="KPG09_003520"
     CDS             826..2745
                     /locus_tag="KPG09_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG0968081.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVKRGAQ
                     ARSTKQKARLQRFDELMNRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(2987..3334)
                     /locus_tag="KPG09_003521"
     CDS             complement(2987..3334)
                     /locus_tag="KPG09_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBG0968082.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(3321..5249)
                     /locus_tag="KPG09_003522"
     CDS             complement(3321..5249)
                     /locus_tag="KPG09_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBG0968083.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTTLPIVLSKVKNNEQE"
BASE COUNT         2098 a          829 c          775 g         1754 t
ORIGIN      
        1 taatactggg ggataatatg agtaaaaagt caaaacgaag aaaaattaat aaattaaaat
       61 taacgattgt aataactgta ttatttcttg ctttattatc tatatatgag atactgttct
      121 ctaatcccaa caaaaaaggt aatataaaga gtcaagaaac tatgcagaat aaaaataata
      181 caacagatga ttctaaaaat aatcaaagct ctaccaaaaa gcaggaagat aataatatca
      241 agcctacttt ttcatattct tcaataccag ataatataaa aaataaaatg attggtaaat
      301 ctatgcctac tgatgaacct ataagctttg atagtctatc ttatttaaaa cttacatatt
      361 atggatttga tgaaaaaact catcaaggag aaatgatagt aaacagtgaa ctagctccag
      421 aagtagtgga tatttttaag gaattatacg aaaaaaaata tccaattgaa aaaattaaat
      481 taatagatga ctatgatgct gtagatgaaa aatctatgtc tgataataat acctcatcat
      541 tttgttatag aactatagct ggtacaaatg tagtttcaaa tcatggtaaa ggacgtgcta
      601 tagatataaa cccattacag aatcctcaag tttctggaaa tgatgttaca cctaaaataa
      661 gtacagttta tgctgatagg tctagcacaa aatttggaat gataaaaaaa ggtgatgatt
      721 gttataatgc ttttgtaagc agaggttgga gttggggtgg ctattggaaa aacccagatt
      781 atcaacattt tgaaaaataa tagattagaa ataaaggtga attatatgaa tttaatgaca
      841 ttggaaaata taagtaaaag ctattctgaa aaaaaattac tagaaaatat atctcttgga
      901 ataaatgagg gggaaaaaat aggacttatt ggtgtaaatg gtactggtaa atctacctta
      961 ctcaaaatta ttgcaggagt tgaagaaagt gagactggta atataacaaa aactaatggt
     1021 attaggatag aatatctacc tcaaaatcca acttacaatg aaaattcaac tgtacttgaa
     1081 caagtattta atggaacttc tgaagaatta aagctacttg gtgaatatca agaaatccta
     1141 gaaaaactta attcaaactt tacagaggaa ttaaataaaa aattattatc agtacacgaa
     1201 aaaatagatg ccttaaatct ttgggattta gaaagtgaag ctaagtcagt acttacaaag
     1261 cttggtataa atgactttaa tcaaaaaata gcagaacttt ctggtgggca aagaaagaga
     1321 gtctctctgg catcagccct tattacacct tgtgagtttt tgatactaga tgagcctaca
     1381 aaccatctag acaatgatac tatagattgg cttgaggaat atcttaattc aagaaaaggc
     1441 tcattgctta tgataactca tgatagatac tttttagacc gtgttacaaa tagaattatt
     1501 gaactggaca agggtagatt attcagttat gatgggaatt attcaatatt tttggaaaag
     1561 aaaatggaaa gactttcgct agaagcaagt attgaagata aaagacaaaa tttaataaga
     1621 acagagcttg cgtgggtaaa acgaggagct caggctcgta gcactaaaca aaaagctcgt
     1681 ttacaaagat ttgatgaact tatgaataga gattctttta aacctgatga aaatctcgat
     1741 atatctgttg catcaagtag acttggaaat aaaataatag aaatacacaa tatatctaaa
     1801 tcatttgaag aaaatactgt tatagataat ttagaataca ctttaactcg tacagataga
     1861 attggtataa taggcaaaaa tggtatgggt aaatctaccc ttataaatat acttaatgga
     1921 gaaatagagc ctgatagtgg atatatttca attggagaaa cagttaaaat aggttgtttt
     1981 tctcaagacg actctcatat gaatattgat atgagggcta tagactatgt aaaagaagct
     2041 agtgactata tagaaacttc agatggtact aggattacag cttctcaaat gtgtgaaaga
     2101 ttccttttta atggtactat gcaatatact ttgataggta aattatctgg tggtgaaaga
     2161 agaagactac atcttcttaa aatccttatg acagctccta atgtgcttct tttagatgaa
     2221 cctacaaatg atttagatat tgaaacgctt aaaatattag aagaatacct agacgaattt
     2281 aaaggtgtag tagttacagt atcacatgat agatatttct tagatagaat ttgtaataaa
     2341 atatttgcat atgaaggtaa tggaaaaatt catatctata caggtaatta cagtgattat
     2401 cttatttata gagagattca aggaattgaa tttgaagaat ctaaaaggga atcagttaaa
     2461 aaagatgact ctactaccct aaaaaaagaa aaacctaaaa atgataaaaa gcttaagttc
     2521 tcatataatg aacaaaggga gtttgaaaat attgactctg atatagaaaa actcgaatct
     2581 aaaattgcaa cacttgatga aagtactgca aaattttcaa ctgattttac taaacttcaa
     2641 gaaattttag atgagaaatc taaactagaa aaggaacttg aatacaaata tgaaagatgg
     2701 gaatatttaa ataatctagc tgatgaaata gctaataata agtgatatta aaataatctt
     2761 aagtttaata agttttgctt taaatctata ctttataaat attttaaagg tagtgtctca
     2821 aaatgaactt tttagtttta tgagacactc ttttttatga gaaatcaaat atgttttcat
     2881 catttcaaca atacaaaaga ggcttatctc tattttgaga cagcctcttt cataattacc
     2941 ttctaattta tatgctttaa tatatctata ataattctta ttatatctat atatccagtc
     3001 tttcaatata gtgacaatta acttcaatat gatttaaaaa tactaacaac ctttcataaa
     3061 gtggatgaga ttcttctcca tatttagctt ctaggttctt gcctatttct ctaacagtcc
     3121 tttgaccatc aatttgtaaa aaaacaaaac tggaatattc atctagttct atttttttgt
     3181 atttaggtat tctagctttt aactttctaa aaaacttttg aattttatgg tcctgttttt
     3241 caagtatagt aacaacacta ttttcagcta cttcatattc aagtccatca gtaattttaa
     3301 atacaatatc taaaacatca ttactcttgc tcattatttt ttactttaga taaaacaata
     3361 ggtaatgttg taagcacaac taaaactact aataaagcaa ttgccattga atttgttgca
     3421 gcaaatccac ttggtacctt tggagttaca actccagtta cttgtaatat tatacctatt
     3481 aaaccaatga tagaaccacc agcaacaaga ccagaagata aacttatacc attagaaact
     3541 ttaacttctt tttctttttc agttttagat actaattcaa caaataaacg aattaatgct
     3601 ccaactaata ttatagaagt tgttgcaatt ggtaaataga atcctatagc tatagtcatt
     3661 attggtaaat ttaatgcata tagaactatt gccataaata ctccaacaat aatcataacc
     3721 caaggtaaac tacctgacat aataccagaa gttaatgttg acattaagtt tgcttgtggt
     3781 aatgcaaatt gtacattatc accagttgct ctaagttggt cagaaagtaa taaaattact
     3841 cctacaacaa ctataacacc aactatactt gcaattgtaa agtaacgttg catttcgttt
     3901 ttgctaccac caataatata agttactttt tgagattgag tataacctcc agctatagca
     3961 atagcaacaa ctataaatga accaaataaa agtaatgatt tattagcttc taaatcagtc
     4021 catcccatta taacaaacac taatgttaca ataactagag aagctatagt cataccagaa
     4081 actggaagat ttgatgttcc aattgttcct gttaaacgac cagctactat gacaaataat
     4141 aaagataata aaagagatat gatagctcca ataattgcca taacaatgtt accagaaatt
     4201 aagaatgctg ctaaaaatcc aataactact ccacctaata aaagtatcat ttgtatagat
     4261 gaaccttctt ctccttctcc agcacttgat ttagctttaa gtgtttcttt tatagaagcg
     4321 attatagttg gtataagttt tattgcacca ataatacctc cacaaagcat cataccagca
     4381 cctatatatt ttacataact actagatatt gctccgacat ccatttggtt aagaggcatt
     4441 gcaggattat tccataccat agctccatct ttagccatat ctgtaaagta tcctattaaa
     4501 ggtgctatac caaagtttga taaaatactt cctgcaaaca ttgttaaaga aacttcaagc
     4561 cctactataa atccaattcc taaaagtaga ggatttactt caatatcaaa tttccatttg
     4621 tagaatttgc ttcctacaag actcattaca ttgtttgcta catttaagaa tgaaccagtt
     4681 aatacagtaa taaatccact tattacaaat ccaattccca tgtacttaat tgaatctcca
     4741 ccagcctctg aagcaacaag tgtctcagat atagccattg attctggata cataagttta
     4801 ccatgttctt caactattag atagttgtga actatagcag caattcctat accaaataaa
     4861 actccaccaa ctccaacagc taggccttca aagaatgtaa tttggcttcc aattaatata
     4921 actgctggta atacgaaaat aaatccactt gcaacagatt ctccaccact tgacatccct
     4981 tggattaagt ttttaccaag aatacccttt gaacgagcaa atgctccaac aaaagcagaa
     5041 cctattatag ctcctggaat acctgcagca actgtaagac cggctttcat tccagagtaa
     5101 gttgtagatg cggcaaaaat ggcagctaaa ataataccga taatcagtac agctacattt
     5161 ccaccggttc tggacttatc tgtgatatat ggaacgtaat cttttccact aacctcacca
     5221 taagcaccct taggtaattt tttgttcata aaattttcac cccttttcaa tttgtatata
     5281 ttataacata tatacatata aaacttaact ttttattatt ttacaaatag cgagtcaaaa
     5341 ctactaaaat ttaaccctat attattaatt tctactttta gatattttta tgctaatttt
     5401 ctttttattt ttcaattcta ctaaaaacta ttaagatata aaataaaaaa aatttt
//
LOCUS       DAELSY010000204         5453 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.205, whole genome shotgun sequence.
ACCESSION   DAELSY010000204 DAELSY010000000
VERSION     DAELSY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.205"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(91..2721)
                     /gene="cdtB"
                     /locus_tag="KPG09_003523"
     CDS             complement(91..2721)
                     /gene="cdtB"
                     /locus_tag="KPG09_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094607992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin binding subunit
                     CdtB"
                     /protein_id="HBG0968084.1"
                     /translation="MKIQMRNKKVLSFLTLTAIVSQALVYPVYAQTSTSNHSNKKKEI
                     VNEDILPNNGLMGYYFTDEHFKDLKLMAPIKDGNLKFEEKKVDKLLDKDKSDVKSIRW
                     TGRIIPSKDGEYTLSTDRDDVLMQVNTESTISNTLKVNMKKGKEYKVRIELQDKNLGS
                     IDNLSSPNLYWELDGMKKIIPEENLFLRDYSNIEKDDPFIPNNNFFDPKLMSDWEDED
                     LDTDNDNIPDSYERNGYTIKDLIAVKWEDSFAEQGYKKYVSNYLESNTAGDPYTDYEK
                     ASGSFDKAIKTEARDPLVAAYPIVGVGMEKLIISTNEHASTDQGKTVSRATTNSKTES
                     NTAGVSVNVGYQNGFTANVTTNYSHTTDNSTAVQDSNGESWNTGLSINKGESAYINAN
                     VRYYNTGTAPMYKVTPTTNLVLDGDTLSTIKAQENQIGNNLSPGDTYPKKGLSPLALN
                     TMDQFSSRLIPINYDQLKKLDAGKQIKLETTQVSGNFGTKNSSGQIVTEGNSWSDYIS
                     QIDSISASIILDTENESYERRVTAKNLQDPEDKTPELTIGEAIEKAFGATKKDGLLYF
                     NDIPIDESCVELIFDDNTANKIKDSLKTLSDKKIYNVKLERGMNILIKTPTYFTNFDD
                     YNNYPSTWSNVNTTNQDGLQGSANKLNGETKIKIPMSELKPYKRYVFSGYSKDPLTSN
                     SIIVKIKAKEEKTDYLVPEQGYTKFSYEFETTEKDSSNIEITLIGSGTTYLDNLSITE
                     LNSTPEILDEPEVKIPTDQEIMDAHKIYFADLNFNPSTGNTYINGMYFAPTQTNKEAL
                     DYIQKYRVEATLQYSGFKDIGTKDKEMRNYLGDPNQPKTNYVNLRSYFTGGENIMTYK
                     KLRIYAITPDDRELLVLSVD"
     gene            complement(2774..4165)
                     /gene="cdtA"
                     /locus_tag="KPG09_003524"
     CDS             complement(2774..4165)
                     /gene="cdtA"
                     /locus_tag="KPG09_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin enzymatic subunit
                     CdtA"
                     /protein_id="HBG0968085.1"
                     /translation="MKKFRKHKRISNCISILLILYLTLGGLLPNNIYAQDLQSYSEKV
                     CNTTYKAPIERPEDFLKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKY
                     SQTRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFESPEKFAFNKVIRTEN
                     QNEISLEKFNEFKETIQNKLFKQDGFKDISLYEPGKGDEKPTPLLMHLKLPRNTGMLP
                     YTNTNNVSTLIEQGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDDVSKGDSWGKA
                     NYNDWSNKLTPNELADVNDYMRGGYTAINNYLISNGPVNNPNPELDSKITNIENALKR
                     EPIPTNLTVYRRSGPQEFGLTLTSPEYDFNKLENIDAFKSKWEGQALSYPNFISTSIG
                     SVNMSAFAKRKIVLRITIPKGSPGAYLSAIPGYAGEYEVLLNHGSKFKINKIDSYKDG
                     TITKLIVDATLIP"
     gene            complement(4555..5301)
                     /gene="cdtR"
                     /locus_tag="KPG09_003525"
     CDS             complement(4555..5301)
                     /gene="cdtR"
                     /locus_tag="KPG09_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HBG0968086.1"
                     /translation="MDILIFDNDVCFGIKLKEKINNILIKEGFDNDVIRLYYNANLLL
                     KELTEKNKVKIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYVKEKNYRSNFFTIYSDSSLWKISF
                     DEVIYFETSAIPHKIKLVTTSRIFEFYKSLRSLSDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT         1734 a          813 c          643 g         2263 t
ORIGIN      
        1 aaataaaaaa taccttataa agttaatttt ttagtacttt ataaggtaaa tatatattgt
       61 attgtgggga caaatttaaa tcatttgata ctaatcaaca ctaagaacta ataactctct
      121 atcgtctgga gtaattgcat atattcttaa tttcttgtat gtcataatat tttctccacc
      181 tgtaaaataa ctcctaagat taacataatt agttttaggc tgatttggat ctcctaaata
      241 attacgcatt tctttatctt tagttccaat atctttaaat ccagaatatt gtaaagtagc
      301 ttcaactcta tatttttgga tataatcgag agcttcttta ttagtttgtg ttggtgcaaa
      361 atacatacca tttatataag tatttcctgt acttggatta aaatttaaat ctgcaaaata
      421 tattttatgt gcatccatta tttcttggtc agttggaatt ttaacttctg gttcatcaag
      481 tatttcagga gtactattta gctctgtaat agataagtta tctaagtatg ttgtaccact
      541 accaattaat gttatctcta tattagaaga atctttttca gtagtttcaa attcataact
      601 aaattttgta tatccttgtt ctggtaccaa ataatccgtt ttctcttctt ttgcttttat
      661 ctttacaatt attgaattag atgttaaagg atcctttgaa tatccactaa aaacataacg
      721 tttataaggt tttagctcag acatagggat tttaatcttc gtctcaccat ttaatttatt
      781 tgctgagcct tgtaaaccat cttgattcgt agtattgaca ttactccatg tactagggta
      841 attattataa tcatcaaaat tagtaaagta agttggtgtt tttataagta tattcattcc
      901 tctttcaagt ttaacattat atatcttttt atcagacaaa gtttttaaac tatctttaat
      961 cttattggct gtattatcat caaatatgag ttcaacacaa ctttcatcta ttggtatatc
     1021 attaaaatat aacaaaccat cttttttagt agcgccaaaa gctttttcaa ttgcttctcc
     1081 aattgtaagt tcaggtgttt tatcttctgg atcctgtaaa tttttagcag taactcttct
     1141 ttcgtaagat tcattctctg tatctaatat aatagatgca gaaatactgt caatttgact
     1201 tatatagtct gaccaactat ttccttctgt tactatttgt ccagaactat tttttgtacc
     1261 aaaatttcca cttacttgtg ttgtttctaa tttaatttgc tttccagcat ctaatttttt
     1321 taattgatca taatttatag gaatcagtct agagctaaat tgatccattg tgttaagagc
     1381 tagaggtgaa agcccttttt tgggataagt atctccagga gatagattat tgccaatttg
     1441 attttcttgt gctttgatag ttgataatgt atctccatct aacactaaat ttgttgttgg
     1501 tgtcactttg tacataggtg cagtacctgt gttgtaatat ctaacatttg catttatata
     1561 tgcagattct cctttgttta tacttaatcc agtattccat gattctccat tactatcttg
     1621 aacagcagtt gaattatctg ttgtatggga ataatttgta gttacattag ctgtgaatcc
     1681 attttgatat cctacattaa cagacacacc agctgtatta gattcagttt tactgttagt
     1741 agtagctctg gaaacagttt taccttgatc agtagaggca tgttcatttg tagatataat
     1801 taatttttcc atacctactc caacaattgg atatgctgca actaacggat ctcttgcttc
     1861 agtctttata gccttgtcaa aagaacctga agctttttca taatctgtat atggatctcc
     1921 agcagtattt gactctaaat aatttgatac atatttctta tagccttgtt ctgcaaaact
     1981 atcttcccac ttaactgcaa ttaagtcctt aatagtatat ccatttcgtt catatgaatc
     2041 tggtatatta tcattatctg tatccaaatc ttcgtcttcc caatcagaca tcaactttgg
     2101 gtcaaagaaa ttgttatttg ggataaatgg atcatctttt tctatattag aataatctct
     2161 taagaataaa ttttcttctg gtataatttt cttcatacca tctaattccc aataaagatt
     2221 aggtgatgat aaattatcta ttgaacctaa atttttatct tgtagctcta ttctaacttt
     2281 atattcttta ccctttttca tattaacttt aagtgtattt gatatagtac tctcagtatt
     2341 tacttgcatt aagacatcat ctctatcagt tgataatgta tattcaccat ccttagaagg
     2401 aattattctt cctgtccatc gtatagattt tacatctgat ttgtctttat ccagaagttt
     2461 atctactttc ttttcttcaa attttaaatt accatctttt atgggtgcca ttaattttaa
     2521 atctttaaag tgctcatctg tgaaataata tcccattaat ccattgtttg ggagtatatc
     2581 ttcatttaca atttcttttt tcttattaga atgattactt gtactagttt gagcatatac
     2641 aggatatact agtgcttgac taactatagc tgtaagtgtt aaaaaactta ataccttttt
     2701 attcctcatt tgtattttca ttgtttctcc tccttaaatt tgaaaaatat taaattattt
     2761 atgtgaaaaa atattaaggt atcaatgttg catcaacaat taattttgtt atagtaccat
     2821 ctttgtaaga atcaatttta ttgattttaa atttgcttcc atgatttaaa agcacttcat
     2881 attcacctgc ataacctgga atagctgata gataagctcc aggagaacct ttaggtatag
     2941 ttatacgtag tactattttt cttttagcaa atgcactcat attcacacta ccaatactag
     3001 tactaataaa gtttggataa gacagtgctt gtccttccca ttttgattta aaagcatcta
     3061 tattttctag tttgttaaaa tcatattcag gggaagtaag agttaaacca aattcttgag
     3121 gaccagatct tctatataca gttaaattag ttggaatagg ttcacgtttt aatgcatttt
     3181 caatgtttgt gattttagaa tctaattctg ggttaggatt atttactgga ccatttgata
     3241 ttaaataatt attaattgca gtatatcctc cacgcatata atcatttaca tcagcaagtt
     3301 cattaggtgt taatttatta ctccaatcat tataatttgc tttaccccaa gaatccccct
     3361 tacttacatc atctttaaaa tcaagactac ttacaacaga tgcttctgct ttaatatagt
     3421 gcttcccatc tataactata cgaacaattt tatctatttt tatactatat ccttgctcta
     3481 ttaatgtact tacattgtta gtatttgtat atggtaacat accagtattt ctaggtaatt
     3541 ttaagtgcat aagtaatggt gtaggttttt catcaccttt tccaggttca tataaagaaa
     3601 tatctttaaa tccatcttgc ttaaataatt tgttttgtat agtttcttta aactcattaa
     3661 atttttctaa tgaaatttcg ttttgatttt ctgttcttat tactttatta aatgcaaatt
     3721 tttctggaga ttcaaaataa tagacataca taggtttatc tattttattt tttgatatag
     3781 catttcgaag ttctttatat tctttttctc gagaatttgc ttctatttga taatcataaa
     3841 aataatttct tgtctgagaa tatttactta tttctacaga atctttttta tatgattcta
     3901 atgcttcttt ttcagatctt tcaagttttt gctctattct ttctgcttct tttctttccc
     3961 attctttagc cttttcttta tctttaagaa aatcttctgg tctttctata ggagccttgt
     4021 aagtagtatt gcaaactttt tcactatagc tttgtaagtc ttgtgcataa atgttattag
     4081 gtaacaaacc acctaaagtt agatataata tcaacaatat agatatacaa ttactaatcc
     4141 ttttatgttt cctaaatttt ttcatttatt ctccctccca atattagttt aattaactct
     4201 tgaactttaa tttatttaga ttatataatc taaataagaa ttttttatat aaatttcata
     4261 aaatatgtaa ctatatatca taaaaatatg tatttatatg atatgtcagt acaaaataac
     4321 aactgaatat tttaaagaga ctatttcata cttttatgat ttaatgagct atcttgttga
     4381 atcataatct aggaacaatg gttgcaatgt ctaatagaaa ttgtatatga tatagtcatt
     4441 atgtgttttt tcacaaaacg attattatat agaggattct taggaactac tttttttgta
     4501 taaaaaaaga gatggttcaa attcttatct tgagacatct cttttttcta tttattatgt
     4561 tttaataatg ttctttaaaa tatttctata tgtattagat atacgacata tatggccatt
     4621 actcatttta atattatttt tctttaggtc gagagaaaca atatgatatt tattaactac
     4681 aaaagattta tgtacacgaa taaagcatgc atctaaatct gataaagacc ttaaactttt
     4741 atagaattca aatattcttg aagtagtcac taattttatt ttatgtggaa tagcgcttgt
     4801 ttcaaaatat ataacttcat caaatgaaat tttccatagt gaggaatctg agtagatagt
     4861 aaaaaaatta gagcgataat ttttttcttt aacgtatctt ttatgagcaa ttttaataca
     4921 agcttttatt ttactttcag caatttccat atcatgtttt aatataaagt ccataacttc
     4981 taacctataa tcgaagatcc ctaaaatcat ttctatatga tttgtaagaa atatgattgg
     5041 actaatataa tctgattctc taattttttg tgctatccat aatccatcac ataattcatt
     5101 acttatcttg tattttgcat ctacaactat aaaatatatt tttactttat ttttttcagt
     5161 aagttctttt aataacaaat ttgcattata atataatctt ataacatcgt tatcaaaacc
     5221 ttcttttatt aaaatattat ttattttttc ttttagtttt attccaaaac aaacatcatt
     5281 atcaaaaatt aatatatcca ctaaataccc tcctataaaa aattcaaaag taaaaaaact
     5341 aaaagtattt tataaaaaat acgtaaaata acactaggag ataaataaaa ttaaggatag
     5401 ttagaattaa ggtatattta tgcttgttta tatagtatat cttatactta tag
//
LOCUS       DAELSY010000205         5449 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.206, whole genome shotgun sequence.
ACCESSION   DAELSY010000205 DAELSY010000000
VERSION     DAELSY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.206"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            305..1831
                     /locus_tag="KPG09_003526"
     CDS             305..1831
                     /locus_tag="KPG09_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG0968087.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NNGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1859..2416
                     /locus_tag="KPG09_003527"
     CDS             1859..2416
                     /locus_tag="KPG09_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968088.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            2938..3981
                     /locus_tag="KPG09_003528"
     CDS             2938..3981
                     /locus_tag="KPG09_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968089.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKG
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            4092..5234
                     /locus_tag="KPG09_003529"
     CDS             4092..5234
                     /locus_tag="KPG09_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0968090.1"
                     /translation="MSEEKNPKENTLDEPKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         2315 a          444 c          949 g         1741 t
ORIGIN      
        1 aaaaatatta aacgaactaa atcaataaaa aagttggtgg ctaacaattt ataattgtta
       61 gcacaatata atcttatgta ttgaaatcta gaatatcctc atttttactt agcataaata
      121 ctattgatta agaaaataat atagaaatta taaatatata taagtttagt ctggtgtata
      181 aaacaagaaa actaagtgat ttttcattta gctttttatt aagcttattt tttatactat
      241 ttataataaa taaactccat gatgggaaaa ctattactat ttaatatgta aaggagtatt
      301 ttaaatgaat gaatcatatt ggtttttaaa ctcaagtcca aaagaatata acaaattagg
      361 tgaaaatata aaaacagatt gtcttatagt aggtggtgga ataactggat tgacaactgc
      421 atatttatta gcaaaagaag gtaaaaaagt agtgcttgtt gaggctgata aaataggata
      481 tggaacttct ggaaggaata ctggtaaggt aacttgtcag catgatatat tttactctaa
      541 gatagagaaa aaatatggat tagataaagc aaaatcatat tataatgcaa ataatgaagc
      601 tttaaattta gttgaacaaa taatagaaga aaacaatata aaatgtgatt ttaaaaggga
      661 aacttctttt gtatttacag aaaaagagga tactataaag aatattaaag atgaatatcg
      721 tacatgtaaa aaaataggaa taaattgtga gtatcatgaa actatagaaa atattccatt
      781 agatataaag ggggctatta gttttactaa tcaaggtcaa tttaacccaa agaagtatat
      841 tgatggattg gcaaaagcag cagtaaattt agggctaaaa atatatgaaa atacaccagt
      901 agtagatctt gaaaaaggaa aaatatgtag ggttaaaaca agagaagata atataataga
      961 agctgagaat gttataattt catctcatag tccatggtat gatggattga atttttattt
     1021 tgcaaaagaa tatgcagagc gtgcttattt aatggttgca gttttggaaa ataaattagc
     1081 agatggtatg tttataagta tagatgaccc atcaataact tttagacaat ataataatgg
     1141 aagtgaaaat ttactgattt ttggaggagg agaccataaa gttggccagg gaggaactga
     1201 gaaagaaata tttgatgatt tagagcatta tggtaaagaa gtctttaagg taaaagattt
     1261 taaggggaaa tggtctgctc aagataatat gagttttgac aatgttcctt atattggtta
     1321 tatcaacaaa agagaagaca atatttatgt agctacagga ttctctaaat ggggaattac
     1381 taatggaact gctgctggaa taataataaa agatttgata ataaataata attctgatta
     1441 taaagatact tttaatccat ccagattagg tagctacttt tcaaaagatt ttataaagga
     1501 aaatgcaaac gtagcaataa attatgtaag tggaaaatta aaaataggta gtggagatat
     1561 gcctaaaaat aatggagaag gaaagattgt taatattgat ggaaaaagat atggtgtata
     1621 taaggatgat aatggagatt tttatattgt agacacgaca tgtactcatt taggatgtga
     1681 acttaatttt aacagtgaag aaaaaacatg ggactgtcca tgtcatggtt ctagatttga
     1741 ctataaggga aatatacttg aaggaccagc tcttaagcca ttaaaacttt atggacatgg
     1801 tgataatgat gttaatccta agttattata aaattatttt taggaaggta aagtgtatat
     1861 gaataaactt ttaaagccaa taactatact attgtatata gtttatattt tatctgccgt
     1921 attttttgta gcaaacaaag aatatggaag tctaggtcta gtagcaatta gtttagttgg
     1981 tactttcatt ttgagtttta taaataagca aactgaaaga ttattgagta aagaattata
     2041 tataatgctt gctttattta taatgttttc atcattgtta ggtacttgtt ttaattttta
     2101 tgatataaat tattatgatg attttttgca tttatggtca ggtataatat cttgtattgt
     2161 ggctttttct ataattaaat acttttatac acaaagagag ataagtaaaa tgagtaaagt
     2221 gtttttagtt atatttttat ttatgtttag tatgggagta gcgagtttat gggaaatagg
     2281 tgaattttta atggatactt ttgttgggac tactacacag gccggggggt tagaagatac
     2341 tatgattgat atggtagatg ctttaatagg cacaattata actattcctt ttatagtaaa
     2401 aaaatctaat atatagtttt aaatttttta tattaattat aatggattta attaatcatg
     2461 ttgagtttca aggtctatat gattaattaa atccattttt gttatttata attattattg
     2521 aatgtatttt tacatataag atataaggtg atagatagaa attgttggat tatgtaaata
     2581 attaatatat aataccatta tgggcaataa atacaaatca aatgataata tagtctattt
     2641 atgtaaatgt tatattgtat cctaagtata atagtaagtt ttttgatagt ggtgtagaaa
     2701 gtaaaaatct actagcctaa attgatgtat ggattagttt aggaacagta attgcatatc
     2761 ttaagtttga tgtcatgttg ggctactaga aataggtcat ctttttcact tgaatatctt
     2821 ttctttaaac atgaggagta tcatatcaaa aacatatgaa aattaagtga tttataagaa
     2881 cagtaatatt ttatatgata gtatagaata gtaataaagt attaggagga acattgagtg
     2941 ttaaaaaagt ggtttggtat tgtgaaaaaa agacaaaaaa gtgagtcagt aaaagaagaa
     3001 ggtgaagtaa tattaaaaaa cgaaaaaata ttatctgaag aaaagttgat agatgaagaa
     3061 ggagttgtag ttaatattga taatgaaata ttaactaaag tagaagtagt aaatgatgat
     3121 aatgaaatag aagaaaaaat aatagaagaa gattggttaa ttagtgaaaa taccataaaa
     3181 ttagatgata aagaagcaat tattaatgat aaaaacatag aattatgtaa aaaagaagtt
     3241 caagttgaaa gtgaaaaaat aggtttaaat aagtttgaag gacttgacca aaatgaaaat
     3301 tataatctag aaaaaaatgt tattgaagaa aaaaaagtaa gtgaatgttt gacagaagaa
     3361 gatttagagt acataaaaga aattaaaata aaaagaggaa aaagtataaa agctataaac
     3421 ttgtatacta aagaagagtg ggtttttgac actcatatac agtgtagtaa aaaactcaaa
     3481 gttccattag gatacataag agaaaattta aaatatggat atatggatta ctttggagat
     3541 gcaataaatt atttaagtga agtattaaat atagatgaat actgcaaagg tgaatggagc
     3601 tatctcgata atagcaaatc tccatctgag atatttaata ttctaaacaa taaaatattt
     3661 agtataaggc tttctaatga aaaaagaaat gaaattttga caaatgataa aattgaagca
     3721 ttaaaaatga attatagatt tgaatgtatt gatgaagaat atgatgaata ttttaaaaaa
     3781 tataagtcta taatcaaaag gggtggaaag aaaaaagttg aattagtaaa taaaaaaggt
     3841 gacattttag aaatatttaa gtctttagaa gaatgtgcca tttatttgca aaaggaaaag
     3901 aatgaagtta tacaaatgtt aaaatatgga gatacaaaag taggaagaaa ctttataagg
     3961 tatagtttga gaagtattta atttcatttt aaattcataa gagtttcatt tctgattcac
     4021 acatttaata ttattttaat gtaaaataat aaaggtgaag tattgcacaa ttaaaattaa
     4081 ggagggtatt aatgtctgaa gaaaaaaatc caaaagaaaa tacattagat gaacctaaaa
     4141 attcagataa tgtaaattct aaaaaagatg atgtgtcaga tgaaaaaaat actgataaca
     4201 aagttaaaaa aagggaaata aagctaaaca acaaaacact taaagctatt gtagcaattg
     4261 ttgcagtagg agctatatgt ttaggtggag gctatgtttt tggtaaagaa gctggaagaa
     4321 aattgcctgc tacttcaaaa cactataata gcagtgaggt agtagctact attggggata
     4381 aaaaaataac aggtgaaatg ttaagaaaga gaatggagcc attattttat ttaaatgcaa
     4441 aaactactat gactgatgac gaaatacgtt catatgaatc aaatatgatt gaaactatga
     4501 ttacatctga tatattatcc tctgaagcag caaaagagaa aataagtata actaatgacc
     4561 aactagatgc tcagtataat aatttaatga gtagtataga atcagcaatg ggaatgacta
     4621 aagaagagtt tttaaaacag tttgatttga ctgaaaaata tataaaagat gatatgaaaa
     4681 aagagctagt agcatcaaaa tatttaaatg aaaaatcaaa agttactgat aaagaagcag
     4741 agaactatta caataaaaac aagagcaatt atttacaagt tagagcttca catattttaa
     4801 taaaaactgt tgatgataaa ggtaagcaag ttagtagtag caaaaaagct gaacttaaga
     4861 aagaagctga agaaatactt aaaaaagccc aagctgggga agattttgct actctagcta
     4921 aaaaatattc tgaagatagt agtgcagaat caggtggaga tttaggtttc tttggtaaag
     4981 gtcagatggt ggaatctttt gaaaaagcag cctttgcatt gaagaaggga gaggtatcta
     5041 acaaacttgt agaaagtgat tatggatatc atataattaa gaaaactgat gaaaaatatc
     5101 aaccatttga agaggtaaaa tcagatttag taagcagttt aacatcagaa aaacaaaacc
     5161 tacttataca aaatttaaaa gaaaaatata atgttaaaat tacagataaa tatcaaatga
     5221 aagctaaaga atagataaag tcaatttatt aaaaaaaaga aaaattaact gtatggataa
     5281 aagctatttt tactctcaat atagagagtc taaagtagct tttattattt tgtaataaaa
     5341 tctatttata tatatgaagt gataaataaa taacatgcaa aaaaatatta aaaagaaata
     5401 tttttaattt gaattttttt aatgaaaatt tacaaaaatg aaaaaataa
//
LOCUS       DAELSY010000206         5333 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.207, whole genome shotgun sequence.
ACCESSION   DAELSY010000206 DAELSY010000000
VERSION     DAELSY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.207"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            114..314
                     /locus_tag="KPG09_003530"
     CDS             114..314
                     /locus_tag="KPG09_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968091.1"
                     /translation="MKKLKTISIFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            complement(522..1112)
                     /locus_tag="KPG09_003531"
     CDS             complement(522..1112)
                     /locus_tag="KPG09_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBG0968092.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(1115..1990)
                     /gene="dpsA"
                     /locus_tag="KPG09_003532"
     CDS             complement(1115..1990)
                     /gene="dpsA"
                     /locus_tag="KPG09_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBG0968093.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEVGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            complement(2066..3457)
                     /locus_tag="KPG09_003533"
     CDS             complement(2066..3457)
                     /locus_tag="KPG09_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBG0968094.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEQLGKQIS
                     LGENISILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNLYMYQYKNLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEYTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIRGGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIVNIGGFKVSSAKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            complement(3461..4597)
                     /locus_tag="KPG09_003534"
     CDS             complement(3461..4597)
                     /locus_tag="KPG09_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBG0968095.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CNLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIERADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     PILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKTKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIERYNIFGGALAYGH
                     PYGASGAIITLHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(4629..5174)
                     /locus_tag="KPG09_003535"
     CDS             complement(4629..5174)
                     /locus_tag="KPG09_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBG0968096.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
BASE COUNT         1795 a          876 c          625 g         2037 t
ORIGIN      
        1 acaacatata gtataattaa aaaaatatat attcatgatt tttatatact atgaaataaa
       61 atatatttaa tattatatag ataaatttga agtttataag aaggaggata agcatgaaaa
      121 aattaaaaac aattagcatt ttttctttga ttataagtgt aattttaacg attggaggta
      181 ttggaatagt aacttattat gtagataatt tgtttattag aggactatca gtatttgttt
      241 taattatgtc ctcttctttt gtatcaacaa cagttagatt gatatttgaa gagtcaaaac
      301 gatataaatt ctaatttaaa aattggatta agtaaataat tttaatatac caaaaattac
      361 ggagattagc tcaaaataga ctttttagtt catgatgcat ttttttgtat gaaattaaat
      421 atattttcat catttcaaca atgaaaaaga ggcttctctc tattttgaga cagcctcttt
      481 gctagtattg tatctaatac ataatattat aagaaccaat tctattgatg tggacttttt
      541 ataacaggct gtagctgttt gttctctaga gcaagttcta tagttggaat tattagctca
      601 gtatgtgcaa tcatagaatt aggctttgct ttgcaattat cttgaccaaa aggtacaaaa
      661 tatatatttt tagaattaag gagaatacca atatttttga aattaaagct tagagcatca
      721 ttcgttgata ttgatattac tagtggttta tcattacgaa gatgaccttt tgctgccatt
      781 aatactggtg aatctgtaat accatttgct aattttgcag cagtatttcc agtacaagga
      841 gctattatta taatatcagc aatcccttta ggtccaaatg gttctgcctc ttcaatcgta
      901 actataggtt tatttcctgt aagttcatat gcttttttca taaactcttc agaatttcca
      961 aatctacaat cgatattttg tgatacatct gaaaatatag tgtgtacatt agctccttct
     1021 ttaaccaaat tctctaactc tataaatatt ttatcataag tacaaaacga accagtaaat
     1081 cctactccaa ttgtaagtcc ttcaagtctc atttctaatc ccttctttca tttataatat
     1141 tttctatagc gttaacaaga atctcaccag atgtctttgg tgaatattta ccaggaagcc
     1201 caaggcaaag agtcgcatta atccctactt ctttaacaac agaataatct actcctccag
     1261 gagaagaagc tatatctata atagttactt cttgtgatac tttttctaat ctttctttcg
     1321 ttaaaataca ctctggtatg gtgttaaaaa tataatcaaa ttgtaaaagt ttttcatcaa
     1381 gttgacttaa agaaatactg gaatatccaa atgcattagc cgtacttctt gcatcattat
     1441 ttcttgcacc aacgcataca tttgcatcca aagcgtataa tttttttgca agtatttgtg
     1501 cacatcttcc ataacctaat actaaacagt tactttgatg tatattgatt atacttcttt
     1561 taatagcttc tgctatagcc ccttcagctg tagcaactgc atttaaaata gcaacatcat
     1621 tcattttcat aaaatcataa atctgaatag agtgttgctc gcaaaattta caaactttag
     1681 ttggtatgtt acctccaaat aatatttgat taggagataa attaattagc aagttatcaa
     1741 tagtagcatc aagacaatca tccgtatttt caatattaac ttgattcttt gtaaatggaa
     1801 ttggacataa aataatggaa cttttggata gtgcttcagc tagtgaagtt gattttccaa
     1861 cgattccatt tataatttca tttgatatcc cataagtgac tacagaatac cctttattaa
     1921 ttagtttttt taccatgtac acttgtcgca ggtctccccc aataacagtt atatcgtatt
     1981 gatttgacat attgtaagcc tcctttacta tatacaatat gtttttatca atacattggt
     2041 tcactaaaat tactaaatat tatacttata acatttttaa taatgctaat ctatctatct
     2101 tttcagaaga ggtatatgga aatgaattta aaaagataat tttctttggg atttcatttt
     2161 tcattagatt atctttcatt ttaatcaaca aatctttctt cgtaattttg tctatagtta
     2221 ttacaaataa tacaatttga tttcctctta tagtgtctga gtacggcaat acaattgcat
     2281 cttcaatttg aggaattttt ttaacttcat tttcaatctt agcagaacta actttaaatc
     2341 ctccaatatt tacaatgtca tcacttcttc cttcaaatat taagtatcca tcattatcat
     2401 aatatccaat atcattgaca gaataaggtt tggttgcatt atatactgca tattctgtat
     2461 tcacaaaaat ttttcctcct ctaatatata tatctattcc tggaaatggt ctgcctacac
     2521 ttagaggttt ttcaattagt tcatcatagc ataaatatgt aatataattt aattcacttg
     2581 ccccatagta taaaataatt tcagactttg gcatatattt ttttagattt cttgcagtat
     2641 attcaaataa taattgagac cctgtaaaaa tacttactac tttgagtact ggttctttta
     2701 agtgtttaac caaaagttga agttttgtag gtataaggta tatgttagtt acattatatt
     2761 gttttattgt ccttatccat gatttacaat taagacctga acttattaca atactacctc
     2821 cttcatataa tactgacatt atagaattta gattccctgt aaaactaagt gttccattaa
     2881 caaacaagat tgattcccca gagatattaa aaataccatt ttgtatagga aaaaatccag
     2941 cccaactctc atatgttcta tataatacct ttggaacact tgtagaacct gaagataaag
     3001 ctcccataca aatactttta tctaaataat ttataaggtt cttatattga tacatatata
     3061 gattaaattt aggattaaaa atagaaaaat taactttttt tgctacttca ttattgttat
     3121 catcatataa atcaataacg tccatagatt catcagatat aatgattgaa atattatttt
     3181 ttaataaaat atcatctaat acttttttag aaagattata atgacaaata ataggaatat
     3241 tttttgaata attaatagca aaaaaactaa tcaactgaaa cattatattt ttagaatata
     3301 ttaaaattga gatattttca cctaaagaaa tttgttttcc tagttgctca ctataactta
     3361 atatattttc ataagtatat ttttctccat ctataattaa aaaattttta tttttatgcc
     3421 tctctttttt tattcttaaa agttcataat agttcataat ctacaccctt tcaagcagta
     3481 aagctgaacc tagaccacca gctgcagcta tagagcaaat tccatatcta cccttagttt
     3541 tttctaatgc ttttaaaaga tgtaatgtaa taattgcacc tgaagcacca tatggatgtc
     3601 catacgctaa agctccacca aatatattat atctctctat taactctgga tactttcttt
     3661 gaaacaacac atctataact gcaaatgctt cattaaattc aacagcagat acatctaaat
     3721 aattaaggct ttctatctct aaaagtttat ccattgcttg tatcgctgat gttggactta
     3781 aggttgcatc tgttcctatt gtgcatgtat taataatttt tgttttaggc ttctttttta
     3841 catgttctaa atacttctta gaacatatta taataaatga agctccatca ttaataagac
     3901 atgaatttgc tgcatttgta atagtttctt ttcctaaaat tggtggcatc ctatctaata
     3961 acctttgact catcttatca cgtatacctt catcataggt actattattt ataggagata
     4021 taatatcctc aagtattttt tcttctcttg cttcttttgc tcttttatga ctttccttta
     4081 cccaaaaatc caagtctgct ctctcaattt gatataactc tgctactctt tccgcccctt
     4141 cgagcatact attttgacta ttatcatcag gagaaaattg agcaactgta taatttggat
     4201 tatttgtatt atatcgctta tcattcttat gatatgtgcg cattggttgt agagaactac
     4261 tttcaaaacc acctgcaata attaaattac actgaccaga tttaaccttt gaaaaagcta
     4321 tgtctatact catcatagca gaagcacact gcatatccac agtaaaagca ggtacttcat
     4381 ttgacactcc tgctgttaaa gtcattaatc tggctatatt ccctcctgtc ccaactgcat
     4441 ttccacatat aatctcatca attttgtcaa tttcatactt tttaattaaa tcttttaaaa
     4501 cacttgctcc tagtaattca ggttgaacaa attgaaaaat tccatttttt agacctatat
     4561 gacttctcaa tcctcctaat ataaaaactt ccttcataca ttacaccctt ccttatatta
     4621 tagatttatc atagatttct tctgagtaca ttgttcagtt tagtgcctaa aaaagatgca
     4681 ataaccgact ttattgtatc tccaattaaa aatggtacta ctgctgcaaa aagtgctgct
     4741 tctactgtca tttgattaaa atagcacata aatactgttc ccataaaata tataataggc
     4801 attccaacaa aaattgttat tagtatatat ctcttaatat cattatttcg tccttttaat
     4861 aaactaataa ttagtgctgc aaacaaaaac ccaaaataaa aaccacctgt tgggcttaac
     4921 aactttccaa taccagatgt acctccacca aatactggaa tcccaattaa acctatcatt
     4981 atatatacac caactgtact aaatgcttgt cttggtgtta aaatcaaagc tatcaaattt
     5041 atcattattg tttgtgctgt tactcctgct ggagtaaatg gcaatggtat atatatgtaa
     5101 gatgatatac acaataatgc tacacatatt gacattttag ttaaatcttg aatatttact
     5161 ttagtatttt tcatttttat aattcccttc atatgtctta tttcatttag tttatatgta
     5221 catatacaaa gcctctatta aagctttgtt ttagtataaa ataaagacgt ttttttgtca
     5281 accattacaa gtatttaggt ttacgtttaa tttataagta aactttatac tgt
//
LOCUS       DAELSY010000207         5294 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.208, whole genome shotgun sequence.
ACCESSION   DAELSY010000207 DAELSY010000000
VERSION     DAELSY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.208"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            234..1037
                     /locus_tag="KPG09_003536"
     CDS             234..1037
                     /locus_tag="KPG09_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968097.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            1049..2179
                     /locus_tag="KPG09_003537"
     CDS             1049..2179
                     /locus_tag="KPG09_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0968098.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLRESIDEPIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEGFVKSYFESYSLIRG"
     gene            2183..3592
                     /locus_tag="KPG09_003538"
     CDS             2183..3592
                     /locus_tag="KPG09_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968099.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDMDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDVEIDYSP
                     ERTHDIWDESNEIDKNTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKAEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            3803..4312
                     /locus_tag="KPG09_003539"
     CDS             3803..4312
                     /locus_tag="KPG09_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG0968100.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLSEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         2208 a          442 c          887 g         1757 t
ORIGIN      
        1 tactagcgtt agattctaat ctaattgtat acttattgaa ataatagaaa tatattggtg
       61 tttataactt aattttattg ttttttagaa atttttttat atattggtat aattattctg
      121 tttaaatttg attaaaaagt atattaatta taataagaaa caattatata aataggaata
      181 gattattata attaatatag atataaaaat aattatagaa agaaaggcac attatggaaa
      241 aattgagagt tattgaaatg ataaaaaata atcccaatat tatagctaca atagataatc
      301 caactgatga aatgaaattg ttagctatta aagaaaatgg gcttgtacta gaatatataa
      361 ataatccaac cagagagatg caagaactag caatagataa taatattaga gcaattaaat
      421 ttatagataa tccaacagaa gatatgatga taaaagcagt aaatgaggga tggagtatac
      481 tggattatat caaaaaccca actgataaag ttattgaaat ggctataaat caagcaggat
      541 gggcaattaa atatgctaaa aatccaagtg aagaattaca actgttagca gtaagaaaaa
      601 attatgattc gataaagttt ataaaacaac cttatgaaag tgtacaagag gaggcagtaa
      661 gaataagtta tgatgccttg agatatatag attcacctag ttataatgca gaacttatag
      721 ctataaaaaa taatgaagca gctattagct ttataactga tttagataaa aataaaatgt
      781 tggaattttt aaaagtgaat attttagtga ttaagtacat tctgaaaaaa gtatctaaag
      841 atgaattaga gagtgtatta aaagaagtat tatccaaaga agatgttgag gaaaagtata
      901 ttagagattt tttgaattgt agtgttatag atagaaatag cgaaagtata ccaatagata
      961 aaattatgtt tatttataaa tatggaagta agacagcaaa aaaaatagct gtagatgaga
     1021 aattaaagat tatgtaggag agagaaaaat gaattttata gacacattaa aaagcgttga
     1081 cttaatatta tccactggag aagtaccttt aatagtaggt gaaagtggaa taggaaaaac
     1141 agctttagca aataagcttg ccaaagaaaa tgattggagt ttaattgtta ttgatggaaa
     1201 cttacttaaa gagggtgaaa taggtggtct tccaactata gaatcttatg taggagttaa
     1261 ttctaatgga tataagactg aaaagaaaac aacagtgtat gctgttcata acaaattaag
     1321 agaaattgat gaagagatat ctaaaagtaa gacagttctt ttatttatag atgagataaa
     1381 tcgttgtgag catacagtac agcaagaact tatgaattta attttaaata gagaaattaa
     1441 tggatataaa ctgcatgatg atgtaaagat tttagcagca atgaatccat caagtaaata
     1501 tggttcagat tttgattatc aagtggtaga tatggatgca gcccaagaaa ataggtttgt
     1561 ttggttaaat atggagagtg accatacaca atggataaaa tgggcaatag atgagggtat
     1621 tgaaagaaaa gttatcgagt ttatttccac ttttccagaa tatctgcata aaataaatga
     1681 agatgatgta agagctactc ctagaagtta tgagagagta tctaaaattt ataaagttta
     1741 taaagaaaaa aataattcta ttcctagagc agtattttta aatgttgtaa agggtaatgt
     1801 aggtaaagtt attgcagaag aatttataag ttttattgaa tcaaactctg aaccattaat
     1861 atcctatgaa gatgtttttt taagagaatc tattgatgag cccattgtag aaagagtaaa
     1921 aaatgagagt catacaagac tttatctatc agctatgaac attctaaaag acttagaatt
     1981 aaatattaag aatgataaat atgaatcaaa ccactatatt aatagattta ttgaattttt
     2041 aaaaatgtat cctgtagatt taatgatagg tattatgaag gatattagaa acagttatat
     2101 tgaagtatat aaaaaagcca tagaaaatga aggttttgta aaatcatatt ttgaatctta
     2161 tagcttaata aggggataat gtatggaaaa ttattttgat aaacaagcaa aagagcttta
     2221 taataaagca agtgaaatta taaatactca ttctatgcta aaggcaaatc gtgctaatga
     2281 gaaatttgat atggatatac cacaagattt taagtatgag ttttttagtc ttgtagataa
     2341 agtgaattta agtcttatgg aagaagaaga taatttttat ggatattttt tgtttcagat
     2401 gtcaagagaa ataagatttg atataagtag tcctacaggc gtgaatttta agggagctaa
     2461 atatgttata tattttaatc caataatctt tttaacgctt gacataaagc aaatggagac
     2521 tacaattaaa catgaaattc atcatatatt atctatgcat ttgatgagag caaaggagtt
     2581 aaaaggaaag tatagtacat tggcaattaa tatggcaatg gatatagtag taaataaatt
     2641 tctaaacaat ttaccaccat atgcaactac cttagagtgg gtaaatttaa aatattcctt
     2701 aaaacttgaa ccatatgaac cttttgaata ttatgtagaa aagattcaga ctgaattaga
     2761 tttgatggaa gtggatgaag atggtgagga agatgatagt gataaatatg gagatgtaga
     2821 aattgattat agccccgaaa gaactcatga tatatgggat gaatccaatg agatagataa
     2881 aaataccctt agagaattta ctgaaaaatt tattaacagt tctcaaaaag gtaaagttcc
     2941 tgcttattta gaaagtatga tatcatcact taaaaatagt aagggtgaat tgccatggaa
     3001 tttatatctt aatagaataa tgggaacagt tgaaaataat aaaaagaaga ctataacaag
     3061 aagaaataga agacaaccaa atcgtttaga tttgagggga gaacttagaa gtcataaggc
     3121 agaaatagca gttgcacttg atgtaagtgg aagtattagt gatgaagaat ttaaacaagc
     3181 tattaaagaa gttcttagta tagtgaaaaa ctataatcat gaaattacaa ttatagaatg
     3241 tgataatgaa attagacgtg tatataaagt gagaactgaa aaagatataa aagatagaat
     3301 taggataggt ggaggtacta gattttctcc agtctttgag tatgctaata ataaaaagat
     3361 taatttgtta gtctacttta ctgatggtaa aggtgaaaat aaactacaag taatacctag
     3421 agggtataaa attttatggg ttatttctgg aagaggagat aaaatttcat taaaagaacc
     3481 ttatggagca gttaaaaaac ttactaaggt taaaacaaat gatgaaatat tagaaataaa
     3541 tgatgtaaga aatgatgggt actcaatgaa tagccaagca ccaattttat aggtattcac
     3601 aggagataag ctgtattaat ttattttgat atgcttgtta taaaaatcta aaaaatacat
     3661 taatcaatgg ttaatgtatt ttttaatttt aataatataa gttttactta catttaagtc
     3721 ttaaaatgtg ttaaaatata atatatagca gatagttttg gcaagatgga tttagttgtc
     3781 aaaactattt tttttattaa ggatggtgat gtttttgttt aagattgatg attatataat
     3841 gtatggtatg acaggagtct gcaaagtcat ggatataaca aatgaacgat ttacaaatgg
     3901 aataaaaaaa gagtattatg tattgagtcc tatttattct aataatacag taataaaaat
     3961 tcctgtagat aatgaaaaag taccaatgag aaagttactt tctaaaatta atgtgctttc
     4021 attaataaat gatattccta atatggatac atcatggata gataatgaaa aattaagaag
     4081 tgagcaattt aaaaaaatac tcagaggtgg taaatgtgaa gagttaataa aattagtaag
     4141 aagtatagat aataacagag agtatgtaaa atctattggt aaaaaaacac atcaagcaga
     4201 tgataatata atgaaagaag cagaaaggtt attaagtgaa gaatttgcaa ctattttaga
     4261 tatttctcct aatgaagtta gttcatatat ttcaagtcat atacctcaat aaagtgtttt
     4321 agtatccttt atttaatcta ttttaagtat tatttatgta tgctggtttt ggtaaaataa
     4381 aatatataat taaattatat aaaaaattaa gaatgattgt attgaagaat tacagaatag
     4441 tgcggagagc ccactccttt aggtgtgaga tggatatcac ttaaagattt aaattgttat
     4501 tgtagatgtt tttaatataa agcaagatta ataagtgtat tgtaagcttt aagtgaaatt
     4561 gatactataa tgagttactt agattttaaa atatataata ttataaaaaa tccttttagg
     4621 ttttgaatga ataagatgga tactatgttg aatagaatcc atcttattta agttttattt
     4681 atgttgttta tttgtaagca ttatttttgt acatgaaaac acagtgtaaa aatatgctta
     4741 gaattatgaa gttttattaa aaatatattc gacaaaataa gactaaaaat attcttaaac
     4801 ttataactaa gataaatatt gtatatttat agtgtagcct gaaaaattgt ggatgtacga
     4861 aatttattta gtattttatt gaaacaaagt agaagaagta ctatagttta tctttgaata
     4921 gattgttatt ttttagtgaa aaagttatta ggttaataca taaatataat agcttataaa
     4981 tagtttaagc atgttaaaaa agtagttatt agagatatat agttacattg cttataagat
     5041 acttaaaatg atagcattca cttatattta gaaaattaag ataaaaaatc atttttatat
     5101 agtaaagcat acctaaattt agaaaataat tgaatttata aaattaatta aatacataaa
     5161 aaagtattca attaacttaa tagatttttt aaaaattaat ttaaattttt tcttctatta
     5221 actattaata ttattttcaa atcaacgcag ttataagaag tgtataattt gattattata
     5281 tactgcatac aaaa
//
LOCUS       DAELSY010000208         5253 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.209, whole genome shotgun sequence.
ACCESSION   DAELSY010000208 DAELSY010000000
VERSION     DAELSY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.209"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..619)
                     /locus_tag="KPG09_003540"
     CDS             complement(<1..619)
                     /locus_tag="KPG09_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0968101.1"
                     /translation="MIKVLICDDDKIVRDDIANIVKESGYVDYVKKVKDGIEAIEFIK
                     SEKIDLLIIDIDMPYKNGIDSAKEILSIDKDIHIVFVTGFADYSLESFKVHPIDFIVK
                     PFKKEKILDSINIAIDHINSHKIAKNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLYTRDEDTIKFYENFDDLKKRLPPNFFMTHRQYIVNLRLIHKV"
     gene            complement(638..1573)
                     /locus_tag="KPG09_003541"
     CDS             complement(638..1573)
                     /locus_tag="KPG09_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0968102.1"
                     /translation="MENNFIFPIVILTLFIFSNLIMNKYRFYKELDILKRGILTFFVS
                     CFVWILFMEGIEYIQLSLGYNFLLEGVLNIILFLILIILSFYIGFKFIRLLDNNNKQE
                     YKLQKLDSKINHKDNIIVKLRSQKHDYLKHLQVVYSLLNTGLAEDAKEYITNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKNISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSAIQKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFLDGFSTKEGQDRGF
                     GLYLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            1781..4366
                     /locus_tag="KPG09_003542"
     CDS             1781..4366
                     /locus_tag="KPG09_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0968103.1"
                     /translation="MKDNLKIALRYIISYKARSLAIALSIILATSLIVGIGTLSRSAQ
                     QAEVDKLKRETGSDHVYFKDINKNQLEYIKSRKDIKNLGITSHYGYTDPNERLAINLE
                     YANKNYLTNQSKLIKGHLPKASNEVVVEKWILNSLGLKPEINQNITFKLYQKEKPETF
                     KVVGILEDRPIEKNKGTCEIFLNLNESKLDKFSYAYIEFNNGIDINTSIDNIVKNTML
                     DENSVGKNKMLIESTRENGTLDNSSKYTAITMSLFSGIVIYSIYVISIYQRIQEYGIL
                     RAIGSTNFKIFKFMFYELFILALIAIPIGICTGIGGAQIFNRTAGNIQFEGNVTVTPF
                     VIPDKIILLSIGSIILTILIISFFTYLKIRRISPIDSIRKTFGTDKNINKVNSLISKL
                     TLNISVTKSISAKNIFRNKKGFIIIILSMSLGGIMIIKENYKYSFSDIQNKNGQEETY
                     MNADFILTNFFLKLNQSNKADSFKDIKGLNDNQIDKIKNINGIDKVKTASILDTRIEV
                     DKLNGLDYYNIINSTPYYKDFPLFIKNKNTGKYTMKQKLRGYNDEMINSLEKYLVSGS
                     INLERMKKENLAILYVPQVSKTNKYKLSYTPGTETPAVDIKVGDTIKVKYPKGEIDQD
                     LYIKLKDNYEYLEYEFKVGAIVSYPFADNYLYSGDQGIDVITSNDYLKKLTGVDKYNV
                     VYVDINKNANVKKINTLLGEIGSESPGTTTANMLMEKENFDKMTARALTYAYGIVAVM
                     FIISVFNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGILSSVITIVV
                     GLIIQFRMYYTYNFVSYGLGFSIDYKIYILVVLANIIVGILATYIPLRKINKISIVEA
                     INITE"
     gene            4378..5049
                     /locus_tag="KPG09_003543"
     CDS             4378..5049
                     /locus_tag="KPG09_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0968104.1"
                     /translation="MPILEILNLGKIYGKKETAVHALKDANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSSGTIKVDGKDICCLSDKKLAKYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKVDEEYIEDIINLLGLSERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            5076..>5253
                     /locus_tag="KPG09_003544"
     CDS             5076..>5253
                     /locus_tag="KPG09_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968105.1"
                     /translation="MLLILDNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRAL"
BASE COUNT         2087 a          662 c          713 g         1791 t
ORIGIN      
        1 taaccttatg tataagtctt aaatttacta tgtattgtct atgagtcata aagaaattag
       61 gaggtaggcg tttttttaaa tcatcaaagt tctcataaaa ttttatcgta tcttcatctc
      121 tagtatataa attaatagct cttgaatttt tctcaaacat gactatatca tcaaaattaa
      181 tcatatgtat ctgtttcctt attttatata caaaaagagt atcttctata aaattatttt
      241 ttgcaatttt atgagagtta atgtggtcta ttgctatatt aattgaatca agtatttttt
      301 cttttttaaa cggcttaact ataaaatcta taggatgtac tttaaagctc tcaagactat
      361 agtcagcaaa acctgttaca aaaactatat gtatatcttt atctatagaa agaatttctt
      421 tggcagaatc aatcccattt ttatatggca tatcaatgtc aataatcaat aagtctattt
      481 tctcactttt tataaattca attgcttcta taccatcctt gactttctta acataatcta
      541 catatccgga ctctttaact atatttgcta tatcatctct tacaatttta tcatcatcac
      601 aaatcaaaac ttttatcaca gacttctctc ctcctaacta ataatatttt ggtattaaaa
      661 ttacaaattc agttaaaaaa tcgtcactat ttacaagtat actataatca tacttatcta
      721 gtatattttt aactagatat aacccaaaac ctctgtcttg cccttcttta gtgctaaatc
      781 catccaaaaa tatactttcg acattatgta ttttaggacc attatttttt atggctataa
      841 tatactgaaa ttcatcttca tatgcatgta atcttatata cttcttttcc ttttgtatag
      901 cagacaaaga ttctattgca ttgtctatta tgtttaatag cactgaactc atatcattag
      961 gttcaatctc taaattatcc ataaaatctt ggatatatac atcaaaagat atgttctttt
     1021 caagacattc ctcatatttt aaggaagcta tagcatctaa ataagatact ttcccatatc
     1081 ttgaattttt tgatttaaag ctatttgata tattcgttat atattctttt gcatcttcag
     1141 ctaacccagt atttaataga gaatatacta cttgtaaatg ttttaaatag tcatgttttt
     1201 gacttcttaa ttttacgatt atattgtcct tatgatttat tttactgtct aatttttgta
     1261 gcttatactc ttgtttattg ttattgtcta gtaatcttat aaatttaaat cctatataaa
     1321 agcttaaaat aattaaaatt aaaaaaagta taatatttaa tactccttca agtaaaaaat
     1381 tatatcctaa acttaattgt atatactcaa taccctccat gaataaaatc catacaaaac
     1441 acgatacaaa aaacgttaat attcctcttt ttaatatgtc taattcttta taaaacctgt
     1501 atttattcat tataagatta ctaaatataa acaaagttag tattactata ggaaatataa
     1561 aattattctc cataattccc ctcccatcta cactttatat gaggattata tcattttaga
     1621 aaaattatgt aaaaagtcaa aatttacatg tacattaatt aaacttgcaa atacacatat
     1681 aaatttgtac aattaagtac tcaaaacttg aagatactta ctttgatata tattttaaat
     1741 aaacatataa ttgatttagc taagaaagga agtggtaaat atgaaagata atttaaaaat
     1801 agcactaaga tacataataa gttataaagc aaggtcttta gcaattgctc taagtataat
     1861 tcttgctaca tcacttattg ttggtattgg taccctctca agaagtgctc aacaagcaga
     1921 ggtagataaa ttaaaaagag aaactggaag tgatcatgta tactttaaag atataaataa
     1981 aaatcaatta gaatacataa agtctagaaa agatattaaa aatttaggta taacatctca
     2041 ctatggctat acagatccta atgaaagatt agctataaac ctagaatatg caaataaaaa
     2101 ttacttgact aatcaatcca aacttataaa aggtcacctt ccaaaagcta gtaatgaggt
     2161 ggtcgtagag aaatggattt taaatagtct gggtttaaaa ccagaaataa atcagaacat
     2221 tacttttaaa ttatatcaaa aagaaaaacc agagacattt aaagttgtag gaatacttga
     2281 agatagacct attgagaaaa ataaagggac atgtgaaatt tttttaaatc tcaatgaatc
     2341 aaaattggat aaattttctt atgcttatat tgaatttaat aatggcattg atataaatac
     2401 aagcatagat aatatagtaa aaaatactat gttggatgag aatagtgttg gaaaaaacaa
     2461 aatgcttata gaatctacta gggaaaatgg tactttagat aactcaagta aatacactgc
     2521 aattactatg agcctatttt ctggtattgt aatatatagt atctatgtta tatcgatata
     2581 tcaaagaata caggaatatg gtatcttaag agctattgga tccactaact tcaagatttt
     2641 caagtttatg ttttatgaac ttttcatact tgctttgata gcaataccta tcggtatatg
     2701 cacaggtatt ggtggtgcac aaatatttaa taggactgca ggaaatattc aatttgaagg
     2761 taatgtaaca gttacccctt ttgttattcc agataaaata atacttttat caattggaag
     2821 tataatactg acaatactaa ttataagttt ctttacatat ttaaagataa gaagaatttc
     2881 tcctattgac tctataagaa aaacctttgg aactgataaa aatataaata aggtcaattc
     2941 tttgatatca aaattaactt taaatatatc agtaacaaaa tctatatctg ctaaaaatat
     3001 ctttaggaat aaaaagggct tcattataat tatattatcc atgagtctag gaggtataat
     3061 gatcataaag gagaattata aatattcttt ttcagatata caaaataaaa atggtcaaga
     3121 agaaacatat atgaatgcag attttattct aacaaatttt ttcttaaaac taaatcaatc
     3181 taataaagct gattctttca aggatataaa aggacttaat gataatcaga tagataaaat
     3241 aaaaaatata aatggaattg acaaagtcaa aactgccagc atcttagata caagaataga
     3301 agtagataaa ttaaatggat tagattacta caatattatt aattcaacgc cttattataa
     3361 ggattttcct ctattcataa aaaataagaa tactggaaaa tatacaatga aacaaaagtt
     3421 aagaggttac aatgatgaga tgataaattc attagaaaaa tacttagtga gtgggagtat
     3481 aaatctggaa agaatgaaaa aagaaaacct cgctatatta tacgtaccac aagtttctaa
     3541 aacaaataaa tacaagctaa gttatactcc tggtacagaa acgcctgctg tagatataaa
     3601 agtaggagat actataaagg tcaaatatcc aaaaggtgag atagatcaag atctttatat
     3661 aaaactaaaa gataattatg aatatctaga atatgagttt aaagttggag ctattgtaag
     3721 ttatcctttt gcagataatt acctatattc tggagatcag ggtatagatg taataacaag
     3781 taacgactat ctaaaaaaac taactggtgt agataaatat aatgtagttt atgtagatat
     3841 taataaaaat gcaaatgtaa aaaagataaa tacactctta ggtgaaatag gaagtgaatc
     3901 tcctggaact acaacagcta atatgctaat ggaaaaagaa aactttgata agatgacagc
     3961 tagggctctg acttatgctt atggaatagt agcagtaatg tttataatta gtgtgtttaa
     4021 tataataaat aacataagtt ataacttaac ttcaagaact agcgaatttg gaatgttaag
     4081 agctattggt atctctgaga gaggatttaa aaatatgata ctctatgagg gaatattata
     4141 tggaatactc tcaagtgtaa ttaccattgt ggtagggctt ataatacagt ttagaatgta
     4201 ttacacgtat aattttgtat catatggatt gggattttca atagattaca aaatatatat
     4261 cctagttgtt ttagcaaaca taattgtagg tattctagct acatatattc cattaaggaa
     4321 aataaataaa attagtatag ttgaagctat aaacattaca gaataggagg attaattatg
     4381 cctatattag agattcttaa cttaggtaag atttatggaa aaaaggaaac agctgtacat
     4441 gcactaaagg atgcaaattt aaaaataaat aagggtgaat ttgttgcgat aattggccca
     4501 agtggaagtg gaaaaagcac atttctacat ttagtaggag gtcttgagag accaagtagt
     4561 ggaactataa aagtcgatgg taaagatata tgttgccttt cagataaaaa attagcaaaa
     4621 tatagaagac aaaaagtagg ctttgttttt caacagtata acctaattcc agtattaaat
     4681 gtaaaagaaa atatagaatt accactaaag ttagacaata aaaaagttga tgaagaatac
     4741 atcgaagaca ttataaattt attgggtctt agtgagagga aaaatcatct tccaaatcaa
     4801 ttatctggtg gtcaacaaca aagggttgct attgcacgcg ctttatctgc aaaaccaagt
     4861 ataatcctag cagatgaacc aacaggaaat ttggatagta aaactacgga agaagtcatg
     4921 gatttactaa aaagttctat aaaaaaatat aaccaaacac ttataataat aacacataat
     4981 gaaaatattg ctagaaaagc agatagaatt atatctataa tagatggaga gcttaaatta
     5041 acactctaaa actatttata gaaaagagga tttaaatgtt attgatttta gataatttaa
     5101 atttaaactt agattttgac tacagaataa taagagaaga aaatgatgat gtagatatat
     5161 ttatagatat taattatcgt agcttagata tagatactga tggttctaac ttatttaatt
     5221 ctagaataca atttccattt gtaagagcat tga
//
LOCUS       DAELSY010000209         5231 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.210, whole genome shotgun sequence.
ACCESSION   DAELSY010000209 DAELSY010000000
VERSION     DAELSY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.210"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            490..1797
                     /locus_tag="KPG09_003545"
     CDS             490..1797
                     /locus_tag="KPG09_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968106.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLVLDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQSKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1883..2145
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2271..3074
                     /locus_tag="KPG09_003546"
     CDS             2271..3074
                     /locus_tag="KPG09_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0968107.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINKTLKEMKKDGTFDKIYDKWFK"
     gene            3112..3723
                     /locus_tag="KPG09_003547"
     CDS             3112..3723
                     /locus_tag="KPG09_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG0968108.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3755..4426
                     /locus_tag="KPG09_003548"
     CDS             3755..4426
                     /locus_tag="KPG09_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG0968109.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            4725..5087
                     /locus_tag="KPG09_003549"
     CDS             4725..5087
                     /locus_tag="KPG09_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBG0968110.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
BASE COUNT         2112 a          461 c          923 g         1735 t
ORIGIN      
        1 ttgtatacta aaagggaata tttacagttt aatggcagtt ttaaaataat aaattaaagc
       61 tgtcattttt atttaaaaat ttttagaaaa agtacaatag tgttaatgtg tgaattagat
      121 aaggtgtata taataattta gatgatatga tataaaatat gttgtaaatt aaatgttttc
      181 tttgacaata tatacacaag gatgataata tctatatgaa taaaagatag tgtgtaattt
      241 gataatatgt aattataaca ttatgatata attacaataa agtatgtaat aatctgtaga
      301 aatttcaagc tagttatcta agttttcgga taactaaatc taaacggaat acataatgta
      361 ttaaataaat gatgatacta aaatgatact atttttatag aaatttattg aggtatataa
      421 ttgatgaaaa taatatatta aattatataa tatattgagt tttacataaa agcgaaggtg
      481 ggaataaaaa tgaaagaaat tgctgatgct ataacatcag agtatttaag ctatggtatc
      541 aatgaagata ttagtatcta tgaaggtaga ttttgcatat atttagataa aaaatataga
      601 tgtaatggga aaatttatta taaaatgaca cctccaatat ctataagttt taaagcagac
      661 ataggttgtg ttgaagagat agacaatgaa gatgacaatt tagttttgga ttatgataat
      721 gctattcttg aagttcatgg atacaaaatt atttctataa caataaatac tttaagtgaa
      781 tttagtgtag aaggttatat caatgatgac tgcataaaat ctaagaactc ctatgttgaa
      841 tatgtagatt ttaatataat aaacttagat aaaatacctg gaaaactaat taaatacaat
      901 gataaggtat acgcaggtag aattgaattt gatataaatg attatgttgt aactatagac
      961 aagcgatatg attatagaaa agaattaaaa tcagaattaa agtctaaatc tggagcaata
     1021 ataactcata taggtaggat tagaagaaaa gatggtagaa tttttagaac aaataataca
     1081 ataaatcttt tagatagaat atcaacagct ttgagtttta tgtgtggaag atatgtaggg
     1141 ttttgtttgg ccaaagggta tagaagtggt aatgaagttt atagaatatg gaatgaaaat
     1201 caaatatctc cttttagata tgtaccaaca tggtcagata cactttcaaa ttatcataat
     1261 atggaaaaat atataagcct tatgtgtaaa aagctagaag atttttatta tggttctgct
     1321 ataaagagtg ttgttgattg gtacatagaa tctcttggaa gtgctaccat ggaaaacaat
     1381 ataatatctg tacaaattgc acttgaaact ttgtcatatg taattctagt ggaacaaagt
     1441 aagatactta cagatgaagc atttgactgt aatttggcat ctaaaaatat aagactctta
     1501 cttgatacat gtaagatacc atatggtaaa catgaattaa atatatttga taatataata
     1561 aaaaataaat ttgatgatgg agtggattta gttatttatt taagaaatag catagttcat
     1621 ccaagtagaa agactcatag agctgtgtta gaggttgaag atatttggaa tataatttca
     1681 ataggaacga gatatattga acttgtttta ctctttattt taggttatag aggagaatat
     1741 tcaaacagat tagtagaaag gtgttatggt gaagtggaag ttgtaccttg gaactagatt
     1801 tttatattta ttttaaaata tatattgaca tatattgcct tatgctataa aatcaatatc
     1861 ataataataa ttcagaataa cattaaatgc taagaaaaga aatagtaaag gtagttaatc
     1921 ttaaacagag agtttccgat tggtgagagg agcaatagat tttactttga acacatcttt
     1981 gagcaggact ctgaaagagt agaaatactt tattaggaga tttccggaat ccttgcaccc
     2041 gttatagtgc tagagtataa taattttatt gtacttaaag aggttagtgt cgtgaggcat
     2101 taatgaacag aggtggaacc acgagagtta actcccgtcc tctagcagaa gctagaggcg
     2161 ggagtttttt aatataattt tttaagcgcg cagagaatta tattgcaata gatacacttt
     2221 ttaaaaaata atcaaaatat tttaaaaatt aaaaggcgag ggagaatatt atgaaaaata
     2281 tattaaaaaa agtaggaata tttacaatta tgttaggttt attaggtgga gttgtaggtt
     2341 gttcaaaacc agataatgaa aaagataagg atgcttcaaa ggaatctaaa aaagaagtag
     2401 ttgtagggtt tgataatacg tttgtaccaa tgggattttt agatgaaaag ggagatacag
     2461 taggatttga cgtagactta gctaaagaaa catttaaaag acttggaatg gaagttaagt
     2521 ttcagcctat agattggtca atgaaggaaa ctgaattaaa tgattcaaaa actgtagatg
     2581 tattatggaa tggatattct ataacagacg aaagaaagaa aatagtatca tatacagaac
     2641 catatttaca aaataaacag ataatagtta ctctaagtga ttcaaaaata aattcaaaag
     2701 ctgatttaaa agataaagaa gttggaacac aacaaggttc aacagcactt gatgcagtgg
     2761 aaaaggataa agattttatg aatagcttaa aaggtggagc accagttctt tatgatactt
     2821 atgataaagc acttagagat ttagagatag gtagaactag tgcagttgtt ggtgatgaag
     2881 ttttaattag atattatatg ggacaaaagg gtgaagacaa atacaaggta ttaaaagacg
     2941 attttggatt agaagattat gttgtagcaa cttctaaaga aaatccagaa ctttgtgaga
     3001 aaataaataa aacattaaaa gaaatgaaaa aagatggaac ttttgacaaa atttatgata
     3061 agtggtttaa atagaagata agttagctgt taagaagggg gcactaaagg catgttacaa
     3121 ggtttagaga tagttatagc aatgttttgt ataacattga tagtatcaat tccattgggg
     3181 attggagttg catttttaag attatcaaag aataaattag taagtggtat tacacaatgt
     3241 tatatattga taatgagagg tacaccttta cttttgcaaa tgatagtcat attttacgga
     3301 ttacctcttt taggaattgt atttgacaga tttacagctg gagtagtagc tttcttctta
     3361 aattatgcag catattttgc agaaatattt agaggtggaa tacagtcaat cgatagagga
     3421 caatatgaag cttcaaaagt actaggattt gataaattta ctatgtataa gagagtaatc
     3481 tttccacaag tttttaaaag aatactagca cctatttcaa atgaagtaat cacattagta
     3541 aaagatacat ctttagtata tatattagga ttaaatgata ttttaagaat atctcaaata
     3601 gcaatgaaca gggaagcaag tctgttacca ttatttgagg caggagcgat atatcttata
     3661 tttgtagcta tacttactaa gggatttgaa ttacttgaaa aaaaatattc ttattataga
     3721 taaagttaca atattatagg aggaggtttt ttatatgttg aaaatcaaaa acttaaataa
     3781 atcatttaaa aaaaatagag tacttaaaga tatttctttt gagttagaag aaggtcagat
     3841 tggagtttta cttggtaagt ctggtgcagg aaagaccaca atattaagat gtataaatgg
     3901 tcttgaagaa tttgatagtg gagagattat aatagataat gaggtaataa aaaataagag
     3961 agatatggca aaaatacgag gaaaaatagg tatggttttt caaaatttca acttatttcc
     4021 tcatatgaca gtccttgaaa atataattga atccccagtt aatgttttta aagttcctag
     4081 aaaagaggct gaagagaggg ctagagaact tttaagatta gttgatttag aagataaatt
     4141 aaattcttat ccatttgaat tgtcaggagg acagcaacag agagttgcta tagctaggtc
     4201 atgtgcattg atgccaaaag tcctttgttt tgatgaacct acttctgcac ttgatattga
     4261 tactattcaa agagtagtca atataatgaa tagacttaaa gacaaaggta tgacaatatt
     4321 aataattaca catgatgttg tcttctcaaa taatgttgca gataaaatta taagtataaa
     4381 agatggtata gtagaaaatg ttcaaataaa agaaaaaatt gtttagagtt ttataaatat
     4441 aaactagggt agaattttat aatataaata aagggcaagt aaaatcaaat atactatgcc
     4501 cttttgttat ctcaaaattt aatcttatat tttatgttta agttacatta taatttataa
     4561 taaaaataaa aggactattt aaatatataa tgtgaataaa tgtttaataa tttgaaatat
     4621 gataattttt tatgttgaaa gcttataatg attatgatat aaattggtta tagtatacaa
     4681 tttatgggta taacaataaa aaattagaaa ggaattttta taaaatgatt aaattatatg
     4741 gatatacaaa atgctctact gttaaaaaag ctaaaaattg gcttaaggaa aataatttag
     4801 aatttgaaga tatagatatg gttcaaaacc ctcctagtaa agaggagtta aaatcaattt
     4861 ataagactag tggatatgat ataaagaaat tttttaatac aagtggtatg aaatatagag
     4921 aattgggtct taaagatatt gtaaaaactg aatctgatga taaactttta gaaatattgg
     4981 taagtgatgg tatgcttatt aaaagaccat tacttttgga tggtaaaaat gtattattgg
     5041 gctttaaaga agatgtatgg aaaagtactt tgctaaaaga agattaggaa gagataggtg
     5101 tgttgattaa aattattaat taatacacct ataaatttat gtattaaatc taatctttat
     5161 aaatattaat tagcaaattc aaatcaaaaa aattcatatc aacaaatata ttacttctta
     5221 ttacaaatta t
//
LOCUS       DAELSY010000210         5087 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.211, whole genome shotgun sequence.
ACCESSION   DAELSY010000210 DAELSY010000000
VERSION     DAELSY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.211"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..141
                     /locus_tag="KPG09_003550"
     CDS             <1..141
                     /locus_tag="KPG09_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968111.1"
                     /translation="IQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKLKKIKKEN
                     HH"
     gene            334..963
                     /locus_tag="KPG09_003551"
     CDS             334..963
                     /locus_tag="KPG09_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBG0968112.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            1202..2461
                     /locus_tag="KPG09_003552"
     CDS             1202..2461
                     /locus_tag="KPG09_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG0968113.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLTYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            2720..3715
                     /locus_tag="KPG09_003553"
     CDS             2720..3715
                     /locus_tag="KPG09_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBG0968114.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRRNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            3961..5028
                     /locus_tag="KPG09_003554"
     CDS             3961..5028
                     /locus_tag="KPG09_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBG0968115.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHTGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSEDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
BASE COUNT         2121 a          487 c          854 g         1625 t
ORIGIN      
        1 atacaagata taacattaac aaatttaaaa aatggtgaag taactttaat tgaattggat
       61 gaaatataca agaagatggg atttttattt gttgttagtg aaggcaaatt gaaaaagata
      121 aaaaaggaga atcatcatta gaaatgaatg aaataataaa tattataata gtttataaaa
      181 cctaacttat gtagaatata aatacttaag ttaggttttt ttgtgataaa gtttataagt
      241 actcaatttt ataagataaa taatggtctt aagtgaaaag taaataagtt taaattttag
      301 taagtattat attgcataag aggtgatatt attatgaaaa tagtaaccta caatattcat
      361 aaaggtatgg attcaaataa tagattaact ctaactaaaa tggggttata tttaaaacaa
      421 ttggattgtg atgtaatttg tttgcaagaa gtattatatc ctcaattttt agctttaaaa
      481 gctgttttaa atatggatgg tgtctttgct acaaatgtaa aaaaagtaaa tatgatatat
      541 ggtatttgta catttacaac atctaaaatg ttaaacagta atcatttttt cttaactagc
      601 aaaaaagaac aacggggagc tctttgtata actatagatg catatggaag aattataaat
      661 gtaataaata ctcacttggg acttgatagg caggagagag caaaacaatt agacgaaata
      721 atagactata ggaacagatt agtggggata gttattttat gtggtgattt taatgagaaa
      781 aatgtctttt taagtatgtt taatgatatg gctatttcac taaacaagtc ttacttacca
      841 acatttgaaa aatcaaactc tagaatagat tacatttttg ttaataaaaa tactgaatta
      901 aaaggataca cagtagaaaa aatttacctg tcagaccatt atcctgtaat aggttacatt
      961 taaatttcaa tataaatgaa attagttgtt ataaaataaa caattttttg ttaagaaata
     1021 gtaaaagaaa tataataaaa aatatcaaaa ataaaactaa ttataagaca taaatttaca
     1081 ataaagttac atttgattaa attccttgtt taacaaaagc tattcaaata taatctaagg
     1141 tagttttgaa atgggaactc tattatgcaa atttaagcta gtgtcaaaat gaggtgaagt
     1201 tatgggcaga agaacaacaa gagtttcaag attaggtaaa gaaaaaaaac gaagtgtttt
     1261 aattgtattg gcagctttag ttctaataat gggtggattt acttattttt ttaacagtaa
     1321 atttctgtac aatgggaaaa tcgctaaaaa tgtatatata gaaggagtaa atgtatctga
     1381 tatgactaaa gcagaggcat taaaagctat aacagataag tatactccag aagacttaaa
     1441 tcttacttat gatgggaaaa aatatacaat atcaccaaaa gatattgatt taaaatatga
     1501 tacagaagat gttgtaaaag atgcttatga aagtacaaaa aaagggtcct attttcaaaa
     1561 cttaaaaaaa tatattgata taagagtgaa taaggcaaat atgaagataa aagctgaata
     1621 tgatgaggca aaacttagtt ccaaagtgtc ttcaatagca gatagtataa atgttaagat
     1681 gaaaaatgct tctattagtg ttggaagtgg tggactaagt tatacagact ctgttgtagg
     1741 tagagaattt gatttggcag caaataaaga gtctatatat aatatgataa aaaataaaga
     1801 gcataaaact ttagaactta aagtaaatct tcaaaaacca gatataacaa ctgaacaagt
     1861 aaaaactgta aattctgtta taggtcaata ttcaactact tattcacaag ctgtagaggg
     1921 taggtcatat aatgtaggat tatcagcaag aaaaactagt gatgtattat taatgccagg
     1981 tgaagaattt tcatacaata aacttacagg acctagtaat aaagcaaatg gttacaaaga
     2041 tgcacctgtt atagtttatg gtaagctaga acaatcagca ggaggaggtg tttgtcaaac
     2101 atcttcaaca gtttataatg cggctcttct ttcgggtatg gagataactc aagttacaaa
     2161 tcattcatct gcatctactt atgtgcctaa aggaagagat gctactgtga gcgatggagg
     2221 attaaactta aagtttaaaa atccatataa gcatcctgta tatattaaaa attatgcagg
     2281 tggaggaagt gtatcatcag taatctatgg taattctggt gacaaaccaa atatatctat
     2341 agaggtaaaa caaactggtc agaataaata ttctacatat cgaatattta aagattcaag
     2401 tggaaaggtc ataaaaaaag aacatatatc aaatagttct tataaagaat taaaaaaata
     2461 gataattttt tagagaaata tttgtaatat aaaagggagt gtctcaaaat gaacttttta
     2521 gtttatgaga cactcttttt atgtgaaatc aaatatattt tcatcatttc aacaatacaa
     2581 aagaagcgta tctctatttt gagacagcct tttttgtaaa aattaacact attagttctt
     2641 gttttaatat tacaatcatt aatatataga aaatattcct atatattggt ataattgtaa
     2701 atagatttag ttatataaaa tgtatgagga ggtgacatgg ttgaatttta taggcaatag
     2761 atatgaggta gtaaattctg atgacgtttt agaaattaac aaaatctata aagctagaga
     2821 tgttttttac aggagaaatg tgttaataaa agttataaag cacaataatt atatttgtga
     2881 agattttgtt tcaaatttaa ttgatgaaag tactgcttta gatgagataa actctcctta
     2941 tatactaaaa ataatagatg ttgggattca ctgtacagaa gaaacaacat tatattatgt
     3001 tgtaagtgaa gactttaatg gtatcggact agatgaatta attctaggta attatcttca
     3061 tttagaagca attgtaaaca taatgataca agttttaaaa gctttagaaa tgatacatag
     3121 gaatcattca tatcatggta gtttaaaatc gagtagtata atagtagata cagaatacaa
     3181 tataaaaatt tgtgattttg gaattacaaa agcaaataat ggggttaata caagaagcta
     3241 tggaaacagg agatatttat gcccacatca actatgtata aattatactg ataaagaaag
     3301 tgattttttt gcaacaggtc ttattctatt tgaatctata tttaaaaaat tgccttttgg
     3361 agaatcaaat tcagaagaaa aaatgctaca atctattgat aagggattag attggaatag
     3421 tataaaagca attaatggaa atacagaact tataaatgtt ataaaaaggc ttcttggaag
     3481 aaataataag tatactagag ccaatgatat tattattgat ttaagtaaag taatgtatga
     3541 aaaagcatat atagaagttg aagatgagaa agaagaagaa cagaaacaag acaaagttat
     3601 acaagttaaa aaagaatata aaagtaaaat gagaaaaaaa ttacataaaa aacttgttgt
     3661 tgccggtcta gctatagtca tgatttcaat gattattatt agttccataa tataaaaatt
     3721 gtatattaag tattttaaaa tcaatgtcta taaatgatat acatagaaat tttagtgtag
     3781 tcttaaatta tgaaaggaag tggttattat gttaacaaaa aataatgagg tattaaacgt
     3841 ctaggagtat tttaacaaat agccacctgc ttttacagtt tagaataaaa aagctgtggc
     3901 taaaggtcac agctttttgc tactttttaa aggcaggaga atattaaaaa ggagcaaatt
     3961 atgagcaata taaaaataat agcaagtaat aaatgttgga ttgaagatat agcaaggaaa
     4021 caactagaag atatttcaaa gttaaatgga attttaagag tagtaggatt acctgactta
     4081 catacaggta aaatacctgt aggtttagca gttgagacta aaaatattat ttatccacat
     4141 ataattggaa atgatatagg atgcggaatg actttattta aaactggggt tctaaaaaag
     4201 aaatttaaaa aagacaaatg gattaagtca cttagtaaga ttaaagattt gtccgatata
     4261 gaaataaaaa acacatataa agaagaatgt ccaattttaa atttaggaac tatagggagt
     4321 ggaaatcatt ttgtagaaat tcaatgtata agtgaaattt ataataaaga acaatttgaa
     4381 caattaaaat tttcatctga tgatattatg atgttagttc actgtgggtc aagaaattat
     4441 ggagaagata ttttaaaaaa attttatgat aaagatggac ttgaagttga aagtgagaaa
     4501 gcaatagatt atattaaaaa acatgataat gctttaatgt gggcagagcg taatcgtgaa
     4561 gctatcagta aaaaaattat gcagtcaata ggtacatctg aagatgttga aacaatattc
     4621 tctataaatc ataactttat agagaaaagg gaagataaat ttatacatag aaaaggtgct
     4681 gtttcaagtg aaagaggagc tgtgattata ccaggttcaa gaggcagtct atcatatata
     4741 gttatgccta ctgaaaatac agagatatct ttatattctc tatcacatgg agcaggtaga
     4801 aaatggtctc gttcagtttg taaatcgcgc cttaaaagta agtatagtaa agatactata
     4861 aaacaaacta aatttaaaag tcagattata tgtaatgatg taaatttatt atttcaagaa
     4921 gctccagagg catataagaa tatagagcag attattgaaa gtttgataga atatgaactt
     4981 attcaagtag tggcaactat gaagccattg attacttata aaggataatt tttaaagtta
     5041 aaaaagatta aaattatatt ataaatagct aattttatgg aacattt
//
LOCUS       DAELSY010000211         5076 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.212, whole genome shotgun sequence.
ACCESSION   DAELSY010000211 DAELSY010000000
VERSION     DAELSY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.212"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(127..654)
                     /locus_tag="KPG09_003555"
                     /pseudo
     CDS             complement(127..654)
                     /locus_tag="KPG09_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            938..1504
                     /locus_tag="KPG09_003556"
     CDS             938..1504
                     /locus_tag="KPG09_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0968116.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKND"
     gene            complement(1695..3731)
                     /locus_tag="KPG09_003557"
     CDS             complement(1695..3731)
                     /locus_tag="KPG09_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBG0968117.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLKNVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(3905..4582)
                     /locus_tag="KPG09_003558"
     CDS             complement(3905..4582)
                     /locus_tag="KPG09_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG0968118.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSRLYFDLSTPGPNHNTKIDINPPS
                     FEKPLKK"
BASE COUNT         1727 a          808 c          579 g         1962 t
ORIGIN      
        1 ggtttccagc aattcaagta gtattggata ggttctatcc tatctctaca tataggtttc
       61 cctatatgct gactatttta gctactaact catgactaaa gtcacaagtg tgcatagcta
      121 ttttttaatc aaattattta ttttattact tttagaacgt cttccatact ttttaatctc
      181 acgattgatt atcagatgag caagatttag aatgattcct tgctttccat ttgaaccaca
      241 tagtataaga ccagataaat tattactgta atccattata tatctttgac ttgcaaatga
      301 acccatgctg tgtccaaata gataaattgg caaatcttca ttttctttct ttataatatt
      361 tgttaaagta tacatgtcct ctacaagaca tctaaatcct tctttttcag ctaaatgtcc
      421 aacattttct attatctttg cagtttttcc atgacctcta tggtcattga tatatactat
      481 ataaccattt ttagtaagta ccttggcaaa agtctcatac ctttgtgcag tttcagccat
      541 tccatgtgca atttggataa ctgcctttgg tttttttata ttttcatctt cccatttata
      601 tgtatatata tctaaacctt cctctccttt gaaagtaaag tttgtacact tcataattat
      661 ccctccagat tgattttatt cagtatatta atttatatat tatattacaa gtattttcaa
      721 gtcaaaatct ttctaaaaaa tttaagaatt attaattcct catagaaatt atcattggta
      781 catagaagtt tttacataca attatacaat aatttaatta atagttacat aaaaaagtaa
      841 tactttaata tattttgaca ccatattgac attagaatta attcatgata ttctgttttc
      901 gacaaaacaa cttctaaaaa taaaggagat atatttcatg gattattcaa atgaactaaa
      961 agagctcttt ttaatgaatc aaacatatgc aactcttttt acccttacaa acaaaataca
     1021 aatagaagga gataaatatt ttggtatact tacttcaaga cagtatatga ctattttatc
     1081 aattcttcat ttaccagagg aagaaacaac attaaataat attgcaagaa aaatgggtac
     1141 aagtaaacaa aatataaata gactagttgc taatcttgaa aaaaatggat atgttgatgt
     1201 aattccaagt cctcatgata agagagcaat taatgttaaa gttacagact taggaaaaaa
     1261 ggttatggta acatgtagta ggactggaat aaactttatg gcagatgtat ttcacgaatt
     1321 taccaaagat gaattagaga ccttatggag tttactaaaa aaaatgtatc gttttaatgg
     1381 tgaagaacaa gatggctttg aagaagatgc taattttatg gaatatgaag aaatagataa
     1441 aattaaatct gaagcacttg aagaatttgc aaaaagaaga aatagggtaa ataaaaacga
     1501 ttaatactat cgcatctata ataccacttt tttatagtac aatattttat ataactataa
     1561 tgtcattttt attttttaac aaaacaatca ttacatagat taggtgttct taggaactct
     1621 ttttctgttt tttgtacaaa aaaaggagat gattagactt ttattttaaa gcatctcctt
     1681 tttcgctatt atgattattc tactcctaaa atcatatctt ttaatttttg taaatcatta
     1741 attggttgac tacaactatt actttgacac acgtaataac tagtcttatc atccttcaat
     1801 ctatagttat ttaaaaaacc tataatagtg ttttcttcat tgtatttctt tgctaaaaaa
     1861 gttgcatttg gtataaaatt ttcacttatc aattctttaa atgcaattaa atcagaatct
     1921 tccttaaaaa tacatataat ttccttagta gaataaagtt caaacatcaa tgaaagcata
     1981 taaaagctgt agccagttgg agaactcttt acattatcca catacagttt taattgttta
     2041 tatgacattt cttcaagtct attatctcca gtaattttcg caagtcttat aagattgtat
     2101 aattgaacag aatttcctga tggaatagct ccatcataca aatcttttgg tcttgctatt
     2161 aaattttcac tatctttacc atatatataa aatccacttt tttcataatc ccaaaaaagg
     2221 ttgatacagc tttcattcaa atttaaagcc ttttctaaat atttcatatt aaatgttgac
     2281 tcataaagct ctatatacgc ccagattaaa aaagcataat catctaaata tgctaaatag
     2341 tcggaacttc catctctata tctagctaat aatctacctg actcatttac aagattatta
     2401 tttataaagt taagacactt atttgaatac tctaaatata tgtcattttt taaagtacta
     2461 tatgcctttg ttaaagcaac tatcatcaat gcattccatg atgtaagtat cttgtcatct
     2521 ttatgcaaag atgttctttc ttttctatat tcaaatacct ttttactaag gtcagctatc
     2581 ttttcattat gtctttcata ttctttattt tttataagat taggaatact tttaccttca
     2641 aaatttccac tactagttat atcaaaataa ttattaaaga aagttccatc ttcttcaccc
     2701 aaaacttcaa ttatctcaag aggattaaat gtataaaatt taccttcttc accttcacta
     2761 tcagcatcct gtgctgaata aaacccacct tctttatcct tcatctctct aactacataa
     2821 tctatagttt ttatagcaat ttctttatat aactctttct ttgttatttt atatgcatct
     2881 aaaaaagcta tagtaagcat agcattatca tatagcattt tttcaaaatg aggagccaac
     2941 cactttttat cagtagagta tcttgaaaac ccaaatccaa tatgatcaaa caagcctcct
     3001 ctatacattc catctaaagt tttttccacc atttttaaca catctttatc tttttctatg
     3061 ctataatatt tcattaaaaa cataagattc tgaggacttg gaaatttagg agcattacca
     3121 aaaccaccat acttttcatc ataaatggcc ttaaatactc taactgatga actcaacatc
     3181 tcttttgata aatcaccctc agtatttttt acaccaaaat catccttaag agcttctatt
     3241 atttcatctc cagattttat taatatatcc ctacttgtat tccatttttc agatacattc
     3301 tttaataaat ctataactcc aggtctatta taccttgaat attttgggaa atatgttccc
     3361 gcaaaaaaag gttttttatc tggtgtcatt attatggtca ttggccatcc accactacct
     3421 gtcatagctt ggcaaactgt catatacacg ctatctacat caggtctttc ctctttatct
     3481 acttttattg ccacaaagtt tctattcatt atttcagcaa cttcttcatc ttcaaaagat
     3541 tctttttcca taacatgaca ccaatgacat gttgaatatc ctacacttaa aaatattggt
     3601 ttatcctctt cttttgcttt tttaaaagcc tcatcattcc aactatacca gtttatagga
     3661 ttataagcat gctgtaaaag atatggagat ttttcattta ttagattatt aggttttcta
     3721 ttactgtcca aaataccacc tcatttgtta ttttattata tgttttgcaa taaacaaata
     3781 gatatacact tataataagt gtatacccat ttttaactat acatatttaa tttttcatat
     3841 aattaattct tctagtaaat aatttttata actactaaaa caaaacaaca taaaaaatat
     3901 caaattattt ttttaaaggt ttttcaaaag aaggaggatt aatatctatc ttagtattgt
     3961 gatttggccc tggagtagac aaatcgaaat ataatctaga atcttctgta actccaaaat
     4021 ctctatttga acctgtatct ttaactttat ttaaagcatc tctaaataga gcattatgtg
     4081 cttcttctct gttaagtaag aaatctattg tttcttttac atatttatca tctatttgtc
     4141 tatacaagta ctcataaact accttagctc tttgttcaga agctatattt gaaagtaagt
     4201 cagctactaa atcccctgta actgtaacat aattagcagt ccaaggagct cctgatgagt
     4261 ttattaagac tggtgataga ccagttaata catgtgtttc tatttcgcct gtattaactg
     4321 cattataatc aacatcatgt ccatttaata aatttatagt ttgtgctacc atttccatat
     4381 ggctaagctc ttctgctgct atatcaagaa ataaatcttt tatttgaggg tcttttattc
     4441 taaaactttg ggacaagtat tgcatagctg cttttaactc tccatttgca ccacccaact
     4501 gttcttgcat taaaacagca tattgaggat ttggtctttc tacttttact tcttttaata
     4561 atgctttatc gtgtttaaac atatgaatac ctccgtaaat tattattcaa aattattatt
     4621 tacttaagtt gtaatattat tctaggttat ttgtttattg ttgttatttt ttacaagtgt
     4681 tggtatttca atgttttatt aaggtcagtt ttgttgttta tgttttatta tgtttaattt
     4741 aaatttattc tattatttat tttcggtggc aaaatggtgg caagtttggt tttgtcacca
     4801 tttattttta tttgtaaaaa atatagttta tcaatataac tttattgaaa aatatgtaat
     4861 atttattgac tttcacgtca cgtgatgata taatataaat atagaaagga ggtaagaaag
     4921 tggggagatt agaaagaaga aaaaataaaa gagaaaacaa atttaataaa attaaaaatg
     4981 ctttctcttt cacattagtt ttaattaatc tagtactcgc aatactaaga ttgattaaag
     5041 aattataagt ttcaatccta ggaggaaaat accttc
//
LOCUS       DAELSY010000212         5066 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.213, whole genome shotgun sequence.
ACCESSION   DAELSY010000212 DAELSY010000000
VERSION     DAELSY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.213"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(16..1383)
                     /locus_tag="KPG09_003559"
     CDS             complement(16..1383)
                     /locus_tag="KPG09_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0968119.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIRYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(1633..2097)
                     /locus_tag="KPG09_003560"
     CDS             complement(1633..2097)
                     /locus_tag="KPG09_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBG0968120.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(2102..2731)
                     /locus_tag="KPG09_003561"
     CDS             complement(2102..2731)
                     /locus_tag="KPG09_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG0968121.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADKVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(2765..3652)
                     /locus_tag="KPG09_003562"
     CDS             complement(2765..3652)
                     /locus_tag="KPG09_003562"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBG0968122.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYSLYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(3681..4919)
                     /locus_tag="KPG09_003563"
     CDS             complement(3681..4919)
                     /locus_tag="KPG09_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968123.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYNL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
BASE COUNT         1852 a          818 c          535 g         1861 t
ORIGIN      
        1 tttatattgt atatcttatt cttttattgc tttaggtttt gatagaaaac atttataaaa
       61 tataaagaaa gaaattgctg ttatagtagc agctgttata tctgcaactg gctctgctaa
      121 aattactgca aaaagttttt cattaaagaa tgttgggagt ataaatatta aaggtattaa
      181 aagtacaatc tttctaagta gagctaaaac caaagatatt gttgcttttc caagtgctaa
      241 aaatgtctgt tgacaagcta tttgaatccc aaacataaaa gcaccagcaa aatatatttt
      301 tatactccaa ctagtcatac taagtaactg cggattctta ttaaatatac taacaaaaaa
      361 tgcaggaaat atcattaata aaagccataa aatagtagaa tatgtaaaac atgacaataa
      421 acacaactta aatgtctttt ttactctatc aagttgacct gaaccataat tataacttat
      481 tattggttgt gctccttgtg taacccccat attaggaagc ataagaattt gcattatact
      541 gctcattatg gccattgaac ctactgctaa atctcctcca tacatttgta atttactatt
      601 taaggctata agtactaaac tttctgtaga ttgcattata aatggagaga ttcccaagcc
      661 aagcactggt aagatgatac ttgcttttgg tatcatatac ttctttttta ttttaagtat
      721 acttttcttt ccaaagagaa acataagtac ccaaatagca gaaacgcctt gtgcaattat
      781 agtagctaat gcagctcctt ttacacccat attaaagcca aatataaaaa tcgggtcaag
      841 tattatgttt attaaagcac ctatagcaac tgtcatcatt ccagttgtag caaacccttg
      901 ggtatttata aaagtattca tacccattga aatctgaaca aacactgtac ctattaaata
      961 aatatttaaa tagtccattg aatatctaat tgtagattca cttgctccaa acatccaaag
     1021 taaaggctct tttccaatct taaaacctat agtcaaaagt attgctaaaa tcaccagtac
     1081 agaaaaactg ttactcatta ttttctctgc ctcatcattg ttttgctcac ccatttttat
     1141 agcagctaat ggacctcctc ccataccgat taaataacta catgcagtta ttataattat
     1201 tattggaaag gccactccaa caccagccat agcaacttct ccattttcca ttctaccaat
     1261 aaaaatccta tcgactatgt tgtataaaac atttacaatt tgtgcaataa tagcaggtgt
     1321 agcaagttta aataataatt tacccacact ttcactgcct aagttagtgt ttgaattttc
     1381 cattttaaaa cctcctcaat ccaataatta aaatgtatca ctaaaatttt attctatata
     1441 aaaatttcat tttatcagca gacaaattta gagtactttc cctacaagta atactatact
     1501 atatttcttt tgccatagta aacattttag aactaactga atttaaatac tttatctcat
     1561 tttttattta ttattaaaaa tttttcacat gtactcataa aaaaactatc caagtatatt
     1621 acttagatag ttttatatta tatgtaaaga aagagctgaa actataaccc ctgataacaa
     1681 attgccaaga aatactccca taaaagcatc ttttattctc atttttaata tggaagctat
     1741 agcagatgca gtccatgttc ctgtagttgg cagtggtata cctacaaata gtattatacc
     1801 aattatcgaa acacttttta atttcttcat tcttgagttt atttttctat caattacatc
     1861 agcaatccct ttaaatagtt tcttccctct taaaaattgt aatacatgtc tgaatgtcaa
     1921 tatcaaggga atcgatacca atgtagaacc tattacacta aatacataaa cccatatagg
     1981 atttaagccc attgcaatac ctataggaat ggctcccctc aattctgtta caggaaccat
     2041 agataaaatt aatatgtgta aatatcccaa actattccct cctattctaa gtgacattaa
     2101 gttatgaatt tatttgctct ataacctcac catccttttt actaactaaa aatgcatttt
     2161 tagtcaatga aagcattggt aaatcactct tagagtctga atatgcatat gaattttcat
     2221 aatcaatctc tatcccaagt ttacctaata cagcctttat tctttttact ttttcaattc
     2281 ccttacagtt ttctccaatg aattgatttt tatatcttga attttcataa aaaaactccg
     2341 tgcctataac cttgtctgca agaccataat acttaaaata tttcatatat gcatatggag
     2401 atgcagttat cataattata acattatctt cttctttatt tttgtaaatg acctttttca
     2461 tagattcgtt aatttttttc attatgcaat catcaaaaaa cttttttaaa tcttcttcac
     2521 taaaatctac tattggataa aagattgcct cttttgcctt cttcaagggt atcattctaa
     2581 ttaaatacat aaatagaata aaaataatgt atggcaactt tataatagtt ttaaaggttt
     2641 tattttttaa agagtaaata aaaaaatcta tcatgctatc agtatttata acagtataat
     2701 ctacatcaag taataataca ctttttttca taagtatttc cccatcctat cactaaaaat
     2761 atcactaaaa aatcaatatt attaaacatg taattatcca taagattacg tttattttta
     2821 aagcattatc ttttaggaca atatcagtag gattattttc atttgtagtg ttaattatat
     2881 aattatatct aagtacacca taaactacaa atattgttgt aaatatcata tgtggctttt
     2941 ctggattact tgtagaatac aatgaataac atacaattgt agaacttaat ataactatca
     3001 tcatttgatt tagattttct atgtcataat cttccaatat ttttctatgt tcaactgcat
     3061 catctcttag agtttctatt tcttttttac gttttccaag agctaagtac agagataaga
     3121 aaaatgtaca taaaattatc caatttgata aactaacact tatggctaca catcctgcat
     3181 aaaccctaag tataaaacca agagatattg aaaacacatc taatagtatt atattcttta
     3241 gtttaagaga atataatata ttgtttatca aatagagact aagaattata gaaagattaa
     3301 aactaacaac tgttgataaa cagactgaaa ttaaagctaa tactacaaat agtatattag
     3361 cacttttttt gcttattctt ccacttgcca ctggtctatt acatttttta ggatgttttt
     3421 tatctttatc catgtctata gcatcattca gtgcatatac tgatgatgac attaggcaaa
     3481 aacacacaaa cgcaataaac gtatttaaaa ataaatttat atttagtatg ttgtttgaaa
     3541 aaattatagc tgaaaataca aagaaattct ttatatattg ctttattctc attaacttta
     3601 tataatctga aattttagta agaagcctta tacttgtatt ggtctgtttc ataaacttac
     3661 taccctttca atattatttt ttaaacagga tatgatttta tccctatgtt taaaatttct
     3721 ataaatccat tgacattact gattactata gtaaccaata acagcgatag aaatatatta
     3781 tacatattct tttcattttt tctaagcgct ttcgtaaagt aagccaataa aattataact
     3841 gcaaaactaa aatttatagc cataatactt gaattgacta gattatatcc tactatacca
     3901 tgtaaagtta gattgaatat aattattccc atacatagct taggaactct tttttctgaa
     3961 ttttcaataa ctgataaaac acaaaaacat aaagctaata atgtaagaat tactggtatt
     4021 gtagcaactt ctccattttg caaaaataac ccttttttaa tacttgctcc aggaaaataa
     4081 aaaaccgaat acaataatct ctctataatc attgcaatct tttccaaaat tggttctcca
     4141 cttataaatt tcgtatcata ctgtattaca tttccaaggt tcataaaact ctcataagac
     4201 ttcgttgaaa ctacacaaat atatgccatt aggataaatg taattaaatg ttttaacatt
     4261 tttcctattt ttaatttaaa ctcaaatatc atattaaaaa aatacaaata tatattacta
     4321 atagttattc caaatagagg aatagctgct aattctacta aataagaatt ttttcctttc
     4381 atcttatttg caataactat gaaaaatatt aatgaaaatt ggctatatat aaatctctct
     4441 acatttaaag tcataaataa ctgtggaaaa gaaaatatca ttatcatagt aagtaaaatt
     4501 aaagttaatc tctgcatttt tatcttttta atatgattaa agactaaagt tattgacata
     4561 gcactagtta ttatttgtag acaaattagt ccaaacccat aggcactatt aaaatctgtt
     4621 gtcccaggat ttgctattga agtacaaaat aggtatattg gatatgcaaa aattgatata
     4681 attgctgaaa acaatatgtg cttactattg tcttgactta tagcaaatac atttaaattg
     4741 aataaaaccc ctgtatctgt atctaacaat acatcataag tattgtttaa attataaaat
     4801 atagtagtct tagaaaaaaa tatcttagtt attattaagt aagttattac aactaatata
     4861 aacaaaaaca tctcgttttt atattttaat attttactca tcaatatatc accatccatt
     4921 cattatatag gatttgctga cacctacaat ttattcctta tatttaatag ccatcttcat
     4981 aatgttctta cttgttttat tgtaattgaa taactcttta tttacaacca caatatttac
     5041 tttattgatg ttataatcag tcaagt
//
LOCUS       DAELSY010000213         4980 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.214, whole genome shotgun sequence.
ACCESSION   DAELSY010000213 DAELSY010000000
VERSION     DAELSY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.214"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(113..1705)
                     /gene="hcp"
                     /locus_tag="KPG09_003564"
                     /gene_synonym="priS"
     CDS             complement(113..1705)
                     /gene="hcp"
                     /locus_tag="KPG09_003564"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBG0968124.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKLG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            complement(1765..2490)
                     /locus_tag="KPG09_003565"
     CDS             complement(1765..2490)
                     /locus_tag="KPG09_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0968125.1"
                     /translation="MMRKIIKIDEGKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            2631..3359
                     /locus_tag="KPG09_003566"
     CDS             2631..3359
                     /locus_tag="KPG09_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG0968126.1"
                     /translation="MKTNYRLHMQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEM
                     LECLGVVDKTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLF
                     GEVFACTRLHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKN
                     ILLHNKIELLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRE
                     LGKMRDEGLLSFNKNKFEIFYFQK"
     gene            complement(3523..4794)
                     /locus_tag="KPG09_003567"
                     /pseudo
     CDS             complement(3523..4794)
                     /locus_tag="KPG09_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416360.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
BASE COUNT         1616 a          892 c          605 g         1867 t
ORIGIN      
        1 gtcgtctcaa aattgatttt ttaatcaaat tttaagttta accccttatc ttaattgtga
       61 catacttaaa aatattttaa ctataaatat ctttaagtat agatatattt atttatccta
      121 aaatcttctt catatcttct tcaggagtag atataggtgt caatgcaaat ttatcaacca
      181 atatatctaa tacattctta gataagaatg ctggcaaaga aggccctata tagatatttt
      241 tgatacctaa atgaagtagt gtaagaagta tacaaacagc tttctgctca taccatgaca
      301 gtaccatgct aagaggcaaa tcatttactc cacattcaaa agcatttgcc aatgaaactg
      361 ccactttaat tgcactataa gcatcattac attgtcccat atccattaaa cgaggtaagc
      421 cacctatatc cccaaggtct aaatcattaa atctatattt tccacatgca agtgtcaata
      481 taacagtatc tttaggagct tgttttacaa attcagtgta ataatttctt ccaagttttg
      541 caccatcaca acctccaacc aataaaaagt gtcttatagc tccactcttt acagcatcta
      601 ctactttatc ttgtacagat gaaactgtac ctcttgcaaa accagtagtt aatgtatctc
      661 ctccattaat accagtcata tgaacatctt ctctatatcc tccaagttca agagcttttt
      721 ctattacact agaaaaatct ttatcttcat caatatgaac catacctgga taagcaacta
      781 cttctgtagt aaatactcta tctctataac tatctttaac aggcattaaa cagtttgttg
      841 taaataatat tggagcagga atattatcaa attctttttg ttggttttgc catgctgtac
      901 caaaattacc ttttagatgt ttatattctt ttagttttgg atatgcatgt gctggtaata
      961 actcaccatg agtatatata tttataccct tatctttcgt ttgttctaat aatttgtgta
     1021 aatcataaag gtcatgtcct gtaactacaa taaatggccc tttctcgatt ttcatagata
     1081 cctgagtcgg ttctggtgtt ccataagttt cagtatttgc tctatcaagc aattccatac
     1141 attttaagtt tatttctcca gttttcataa ctagacctaa aagttcgtca atagacaagt
     1201 ctcctccaac tgctattaat ccttcataga aaaattcatt tacgtctttg tctgtataac
     1261 caagtaccat tgcatgatat gcataggcag ccatgcctct taatccaaat aagattaatg
     1321 attttaatga acgtatatcc tcattatcat tccataaatc cttcatatca tagttatcat
     1381 ttctattaca agttgttgaa caatctgaac atctaggtac aagtttactt ttttctacct
     1441 cagttttttc aatttgtctt tttatagctt catcatcaaa acttacattc gttatagtag
     1501 tgaatagccc ctctatcata attttatctg tttcacttgt tggtctattc ttatttccaa
     1561 tagttgctct tgaaagacca atcaacactc ctgtaagctc gtcttgcaag ttagctgtac
     1621 cccccttttt accacaaaca ccatttatag tacaaccttt acctcctgct gtttgttcac
     1681 attgaaaaca aaacattttt tccatttttt attcctccct ttttatttat taaaatttat
     1741 tatatttttc tatgattcac ttaattaaat ttctccatct acaatttttc catctacaga
     1801 tatagtaact atttgccatg gtatcatttt tccactattt tgtagagctg tcttaacagc
     1861 atttactata ccaccacagc aaggaacttc catacgtgtt actactatat ttttaatatc
     1921 attttctttt aaaatctctg tcagtttttc agcatagtca acttcatcaa gtttagggca
     1981 tccgattaat gtaaccttat ttttcataaa tttattatga aaatttccat atgcatacgc
     2041 tgtacagtct gctgctatta aaagacttgc attctttaaa tatgatgcat tagttggtac
     2101 taattttatt tgtactggcc attgattaag ttgagaacct tttatctcta ctatatcatt
     2161 gcttatagat gtactatttg aaacctctct atttattgac ttagactgag aacctggaca
     2221 tccacatgct aaagttttct tttgtgcttt ctttgcttcc atatttgctt ttactgcagc
     2281 ttcatcatat tctgctgctt ctctatactc aaatgaaatc gcatttgttg gacaagttgg
     2341 cagacaatct cccagcccat cacaatagtc atctcttaaa agttttgctt ttccatttac
     2401 cattcctata gcttcttcat gacaagcttc aacacatagt ccacaaccat tacactttcc
     2461 ctcatcaatt ttaattattt ttctcatcat ttatatcacc tcataaaatt tttttaattt
     2521 aaagtattga tttcttatat atttattttt aatacctata tttctttatt tcaaatttct
     2581 tgtagtagga tttttatatc cccttgcttt tataaactta gtatagtaca atgaaaacaa
     2641 attatcggtt gcatatgcaa caaggaagtg attatttgaa aaaattttta gaaaagcata
     2701 tagacctact taaaaaaatt cctctatttg aaggaattaa aagtgatgag cttgaagaaa
     2761 tgttggaatg tttaggtgtt gtagataaga cttacccaaa agatagtata atattctctg
     2821 ttggaagtga agttacttct attggaatta tgcttaaagg aagtgctcat ataataaagg
     2881 aagatatcga agggaatagg aatattgtag ctgaactctt gcctggagat ttatttggag
     2941 aggtattcgc atgtactaga ctacataaaa gtaatgttac agtaaccaca acatcttctt
     3001 gcgaagtttt atttataaag tttaaaagtg taactggtat ttgttcatct gcatgtgtat
     3061 ttcataatag gcttgttgaa aatatgctac aattaatagc tgaaaaaaac attttacttc
     3121 acaataaaat agaattactt tctcgtaaaa caactcgcga gaaattgctt atgtattttt
     3181 caaaacagat agaacaaaca ggtagccacc agtttacaat tcccttttca agaaatgaaa
     3241 tggctgattt tttatgtgtt gacagaagtt ctatgtcaag agagcttggc aagatgcgag
     3301 atgaaggctt gttatctttt aataaaaata aatttgaaat cttttacttt caaaaataaa
     3361 ctagatattt aaacatattt atttcaatat atactatatt agttaattca aattataaat
     3421 atcatatttg aatcttaaat ttatattttt acttaaaaag ttaatagata tctaatttat
     3481 taaaattaag atacctatta actttattaa ttaagtgtac aattagctta ttttctttgt
     3541 agtaattgct gttttatcta taagccaatc ttttccttct actaatcttg aaactaacat
     3601 tgatgatact gtatttccag ttgtatttaa tatagtatta ggaatatcag ttatagtagt
     3661 tataactgtt aacaatggaa ttgcctccac tggaaaaccg aagatgctaa ccacgagcat
     3721 ctcaccaata agtcagccac ctgctacagg agccataaat actccaacaa caaatacagt
     3781 tataaataat tgacctatcg ttgctggatt tgccattcca ctgcctaaaa tacctgataa
     3841 aaatgccact tttattatta ctgctaaaac tgacccatct ttatgtatat ttattccaag
     3901 aggtataact gtttctgata tatcatcagg aactcctata tttttactag cttctaaatt
     3961 tgatggtata catgctgaac ttgaagaagt agctagtgcc gttgctattg gttttatagc
     4021 atttctccag aattgtctaa acccttgttt tcctgcagat aaaaaagcat acagactgta
     4081 aaataataag aaatacatgg ctgctgttag tgaataaatt acaaacaccc ttacatatcc
     4141 ttgaattatt ttgcttccaa gttccccaat tattgtagct gtataacagc ctagccctat
     4201 aggagcataa agcatgacta tacttactac ttttaatatt gcttccgttc cttctgataa
     4261 aaactttctt attgtatttc ccctatctcc catcatagct attgctgttc caactaaaac
     4321 tgcaaatata actaattgta gtaaattgct actggaaaat aaatctccaa aatttttaac
     4381 tgtaatactt gatactactt ggtctaataa cgttcctttt gattctaaag tttctgcatc
     4441 ttgagccacc attaaacttt ttacacttga tacatctaat cctttagacg gattaaataa
     4501 taatgcagat gtaataccta aaaatgcaat tacaagtgtt gttcccataa atactatagc
     4561 tgtactcttc attatttttc caaaacgttc catatttttt atactagcaa cacctgaaga
     4621 aacactaaga aaaactaatg gtactactat cattaataga gcatttatga ataaatctcc
     4681 aaatggtttt agtattgaag cttttggacc aagtactaat cctactatca cccctattat
     4741 agtacatgct aaaagtataa ttgatgactt ataatttttt aaaaagtccc tcatttatat
     4801 tttcctttct aatgttttat attataaatt accctaaatc ctaaaaaaat taaagtaaat
     4861 tatctaatgc gcattaataa tttttgtaat tttccccaac agctacttta gcaaatactg
     4921 gaatatatgt caatttctat agtttttttg tatctttaat attttattaa tagttcatat
//
LOCUS       DAELSY010000214         4964 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.215, whole genome shotgun sequence.
ACCESSION   DAELSY010000214 DAELSY010000000
VERSION     DAELSY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.215"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..454
                     /locus_tag="KPG09_003568"
     CDS             <1..454
                     /locus_tag="KPG09_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0968127.1"
                     /translation="KTIGSLNRNKARLKVARLQEHIANQRKDFLQKLSTKLIKENDII
                     CIEDLQVKNMIRNRKLSRLISDVSWSEFVRQLEYKANWYGRQIVKVGKFFASSQICNK
                     CGYKNEEVKDLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            605..1411
                     /locus_tag="KPG09_003569"
     CDS             605..1411
                     /locus_tag="KPG09_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0968128.1"
                     /translation="MGMILSTLGINLYLAPAKLLSGGVAGICVILYKLFGINQGISSF
                     LMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLTQDIAKYFPIDDTMLQCIYGGAL
                     TGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNTFLFINFFVVCAGAFLFGAKLVM
                     YTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEISKVIMNKMGRGVTFLEAEGAYT
                     QNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNNVEEVKGGGFKDKFL"
     gene            1538..1810
                     /locus_tag="KPG09_003570"
     CDS             1538..1810
                     /locus_tag="KPG09_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968129.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(1973..2545)
                     /locus_tag="KPG09_003571"
     CDS             complement(1973..2545)
                     /locus_tag="KPG09_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0968130.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            2729..3571
                     /locus_tag="KPG09_003572"
     CDS             2729..3571
                     /locus_tag="KPG09_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG0968131.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            complement(3674..4750)
                     /locus_tag="KPG09_003573"
     CDS             complement(3674..4750)
                     /locus_tag="KPG09_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0968132.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
BASE COUNT         1834 a          631 c          786 g         1713 t
ORIGIN      
        1 aaaaacaatc ggtagtttaa atagaaataa agcaagatta aaagttgcga gacttcaaga
       61 acatatagcc aatcaaagaa aggattttct acaaaaacta tctactaaat taataaaaga
      121 gaatgatatt atttgcatag aagatttaca agtaaagaat atgattagaa atcgtaaact
      181 atctcgctta atttctgatg tgtcatggtc agaatttgtt cgtcaattag aatacaaggc
      241 taattggtat ggtagacaaa ttgtaaaggt aggtaaattc tttgcaagtt cacaaatatg
      301 caataaatgt gggtataaaa acgaggaagt taaggattta aatataagag aatggatttg
      361 tcctagttgt aatgaaactc acgatagaga tataaatgca agcataaata tattaaaaga
      421 aggattaaga ctaataacaa ttcaaaataa ataacttatg taagaaccgt aggaactacg
      481 gggatagctt gtggagaatt agtaagacat attttagtat gcaaaattct atgaagcagg
      541 aaccctgtga ctttagtcat gggaggttca gaataacata tttttacaat atggtctgat
      601 tatattgggt atgattctat caaccttagg aataaattta tatctagcac cagccaagtt
      661 attaagtgga ggagtagctg gaatttgtgt tatattatac aaattatttg gtataaatca
      721 gggaatatca agtttcttga tgaacatacc aatatttatt atagctagaa agtattttga
      781 taataaattt ttgctaataa gttttgtcaa tatgttactg ttttctctag cactggggct
      841 aacacaagat attgctaaat atttcccaat agatgatact atgctacagt gtatttatgg
      901 tggtgcatta actggtatag gtatggggtt aacttttaaa gcaagagcta ctgctggagg
      961 acttgatata atagcagcca taatgaaaag aaagtatgat atacctatga aaaatacatt
     1021 tttatttatt aatttttttg tagtatgtgc aggagctttc ttatttggag ctaaattggt
     1081 gatgtacact ttaattacaa tgtatataat ttcttttact atggatatag gaaaagattg
     1141 ctttgataga aaaaaatcta tacttttaat atcaaataaa tatgatgaaa tatccaaagt
     1201 gataatgaat aaaatgggta gaggagtcac ttttttggag gcagaaggtg catatacaca
     1261 aaataagaaa aagatgattt attgcataat atctgcaaat gaaataggta aatttaaaga
     1321 tttaatttat gatatggata gtgaagcttt tatttctgtc aataacgttg aagaagtaaa
     1381 aggtggaggt tttaaggata aatttcttta agattatata tgttaattat atatgttaaa
     1441 aaatgccata tgtattgaga ttatacctag ttagatgtat actgtagtta atggggtaca
     1501 tgaagtactc aataatatac taggaggtat aagaattatg ttaagagaga gatttgtagt
     1561 aagaaatcat cataatccac atacaagtgg gtgttgtggg actggaggag aaggatgctg
     1621 tggaggagat aaccatgaac attcacatca acattctcac agtcatgggc atgaacatga
     1681 gcatgtaaat ggtgtaggtc acgaccataa tcatgatgat ggtttttcag gtactcatga
     1741 gcatagccat gagcatacac atacacatgg tcatgaacat tcagatgggc atacacatga
     1801 acataaataa aaacatttta aatttataag agatttttat tttgtgattc catgaaggtt
     1861 taaataaaaa ttgaatgttt taagtaaaaa ttattgagaa taaataagaa agtgaactgt
     1921 taaagtagat aacttaaata gttcactttt attaattatc taagtattaa gtttaaatcc
     1981 aatgattaac taaaaaagaa gctatatttt cataaggtat tttttctgtt tttaataaac
     2041 tttggacaac taaaccatca actattgaca tgagtaaaaa agaaaatgat tcagcatcct
     2101 gttcattttc gctactatta tatttttcta gtgattcaac aatttcagat ttaagagtag
     2161 ttctccattc aatatatttt aatcttattc tttcctttaa atcattattt gaagtaattg
     2221 cctcacaaat aagatacata tgtaccctac cacgactttc tatagtagat attttctcta
     2281 agattaagtt aaccatttct atttgagaat tttttgattt gacatttttt acacagttta
     2341 gtatagcact agttataact tctaaatgag tatccgcaat atcatatata atatcatctt
     2401 tagatgcgta gtgataatac aaagtacctt tacttatatt tacatctttt gcaatatcag
     2461 caaggctagt attttgaact ccatttttgg ctattaaatt ggttgcagat gataaaatag
     2521 cctgtttcat attatttgtt ttcataaaac ctacctttcc atgtatattt ataacaaatt
     2581 aataacatta taagcttatg aatatttatt gtcaataaag cgagaatcta tatctattga
     2641 ctttttttca ttcaatatat aaaataataa ttaaatcggt cgtacgaccg actgaataaa
     2701 taaaataata tcttatagtg aggttaatat ggataaaata aaattgatag tggacagtgc
     2761 atgtgactta cctgatgata taatagaaaa atacaacata gaagttgttg gtttaaatgt
     2821 atcttttgga gaagaaagtt atatatctgg aaaggaaata gataatgaaa ctttctatag
     2881 aaaaatgagt gagagtaaag tactaccaaa aacatcatgt ccttctcctg ataaattttt
     2941 agaagcatac cattgtcagg aagaaagtgt attggtgatt aatataagtt caggattatc
     3001 tgggacttat aatagtgcac ttttagcaaa agatatgttt gaaaaggaag gaaataacaa
     3061 aaaagttgag gttatagatt cacttagtgg gtcaattgga ataggacaat tagtgttaaa
     3121 agcagcaata ttaataaaag aaggaaaaga aattgaagag attgtttcaa taataaataa
     3181 atataaagat aatcctccat tttttggaac acttgaaact ttagaaaatg ctattaaggg
     3241 tggtagaata aatccaatag caggaaaaat tataaatacc ttgaatttta aggcgataat
     3301 acaagttgca gatggcgttg taactcctat agataaggca agaggtgaag gaaacagttt
     3361 gaaaaagctg attactttag tagagtcaaa aattaaagat aaagaagaaa aggttttatt
     3421 tataggacat gctaattgcc cagaaaaggc tcaaaaggta agggaagtta tggaaaagga
     3481 tgtaaaatac aaagatgttg taatctgtga aattggttca gtgatgggga catatacttc
     3541 aaagggtgca atcttaataa ctgcaatata aataatggat tgaaataaat catagattga
     3601 gaaattaaag ccatataaaa atgtttaaaa catttttata tggcttttta attttatcaa
     3661 attgatttta gaattaacca actacacata tttctttgtc atgtttgttt aatatctcac
     3721 aaccatcttc agtaacaagt actaaatctt ctattctaac tccgaactca cctggtaaat
     3781 atatacctgg ctctatagag aatatcattc ctggttgtac agtatctgta tttatagaag
     3841 atacatctcc tttatcatga acttcaagtc ctatagagtg accagttcta tgagtgaagt
     3901 actgtccata tcccttttca gttatgtagt cacgagcagc agcatctata tcacagaatc
     3961 taacacctgg ttttacaata gcttcagctc tcttattagc ttcaagaact atttcaaata
     4021 tttctctacc tttttcaggt atttctttat agaatacagt tcttgtcata tcagcacaat
     4081 agttatcttt tatacaacct atatctaata ttattgcatc tcctggtttc acaagtgcat
     4141 ttccaggttc accatgtgga ttagctccat taggtccaaa acctataata gggtcaaatg
     4201 aaaatccatc agtacctagg tcttcatata ttttagataa tttaccaaca agttgtttct
     4261 ctgtaagttc accagaaaca gtagctttaa gttgttccat agccttgtca tttaattttg
     4321 aagctattct cattaactct ttttcttctt catctttaca cattcttaaa gtatctatta
     4381 tataagatga atttacaaac ttacttcctc cacctaattc tattaagttt aataagaatc
     4441 tagctggcca gtttttatca acacccatag tagcattttt gtctatatgt tcagttatta
     4501 tttctactgg attttgagta tcattaaacc aaaccatttc aacacctaaa tcttctgaaa
     4561 ctgggaataa ttcatttata aataatttat tattgccatt taaatttaag tataatgcta
     4621 ataatctttc acctggatgg attagtactc ctgtaagata aaatatagaa gtagggtcac
     4681 ttactaacat ttgagaaata tcatcttttt tcatttgttc aagtacagca tttaatcttt
     4741 gagctttcat aataatttcc tcctaatcaa aattcaatac aaatcaattt tagcattatt
     4801 aaacaaaaaa gagtatactt ttcttctaga aatataaaaa aagtttgtta tatatttttt
     4861 atattaagtt taatctctac ttttgcatta aatacggaag taaaatgact ttttattgtg
     4921 aaaattctat aaaagtaata aattaagtct ataaagttat gtat
//
LOCUS       DAELSY010000215         4959 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.216, whole genome shotgun sequence.
ACCESSION   DAELSY010000215 DAELSY010000000
VERSION     DAELSY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.216"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..163
                     /locus_tag="KPG09_003574"
     CDS             <1..163
                     /locus_tag="KPG09_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0968133.1"
                     /translation="QICNKCGYKNEEVKNLNIREWICPSCNETHDRDINASINILKEG
                     LRLITIQNK"
     gene            281..942
                     /locus_tag="KPG09_003575"
                     /pseudo
     CDS             281..942
                     /locus_tag="KPG09_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(1161..2198)
                     /locus_tag="KPG09_003576"
     CDS             complement(1161..2198)
                     /locus_tag="KPG09_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBG0968134.1"
                     /translation="MNKKLIKAKIFIWSILAISLSIVFISLLIGTIISHSFNFPFNTL
                     NPKYNYDNYIVAKNESFSDKINDINVDWISGEVRIKRSDSNKIKLIQKIPSNFSKKKL
                     AKIMVDGNSLSIYDNSTKKFFIGINLPKPTILELYLPNKLYDKITLYTTSADIYSTYI
                     RSNTVEANTISGAIGLSGNINKINLDTTSGHIDITKLNSKNATLKSTSGEIQLSGKID
                     VLNLETTNGNINLKDIYNKNLTCSSVSGKMNLSGVFSDIKAESTSSNIKINSSKMLSS
                     LYCETTSANVDLSIPDNPGFTLNFDKVSGVLDSDFELNRHSDLYTYKNGITNLYLSTT
                     NGNLTIIKNNH"
     gene            complement(2191..2877)
                     /locus_tag="KPG09_003577"
     CDS             complement(2191..2877)
                     /locus_tag="KPG09_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968135.1"
                     /translation="MLDKIRTYIDSSFTGVNETKKVKELKDELFENLKEKYNDQIQDG
                     KTEQEAYNSVISGIGDLSELIESVKEPYSLPSELIEEKKKRALRASIAVALFIISPFL
                     FVVSVVSFGIEPTIAILLMLILIALGVGLLVYNQMTRTNYALYNDNLSNKFKEFEDFE
                     ENYNHINPVYKSFMNAYWIIVVSIYLITSILFNIWSFSWILFIIGAAGVNIVKGIIQL
                     KGYKEEDKYE"
     gene            complement(2889..3209)
                     /locus_tag="KPG09_003578"
     CDS             complement(2889..3209)
                     /locus_tag="KPG09_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0968136.1"
                     /translation="MIIASDMIRGHTETIILKHLLEKDSYGYEINKSILEKTKGLYEL
                     KEATLYTAFRRLEQSGAITSYWGDEVTGARRKYYSITNIGKEKYIKSREEWNNTKALI
                     DILI"
     gene            3570..4910
                     /locus_tag="KPG09_003579"
     CDS             3570..4910
                     /locus_tag="KPG09_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0968137.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLFLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT         1844 a          542 c          745 g         1828 t
ORIGIN      
        1 acaaatatgc aataaatgtg ggtataaaaa cgaggaagtg aagaacttaa atataagaga
       61 atggatttgt cctagttgta atgaaactca cgatagagat ataaatgcaa gtataaatat
      121 attaaaagaa ggattaagat taataacaat tcaaaataaa taacttatgt aagaaccgta
      181 ggaactacgg ggatagcctg tggagaatta gtaagacata tgttagtatg caaaattcta
      241 tgaagcagga accctgtgac tttagtcatg ggaggttcag attttggctg aagatgaaga
      301 aaataatcat ataaatattg agatgcaaag agcttttgag aacgattatt tggaaagagc
      361 tgaatattat cttagtcgtg tacatggtag aaagttagaa gaaggtaaag agtataaaga
      421 aattggaaaa actattggaa tacatatact aaatcatgta aaatataatc acatagaaga
      481 ttatgtaaat tgcctgagat taacaatgga cgagcatcca gatatttatt ctagtaaaac
      541 tgcattatat tttatagagc ttccaaaaat tcataaatct gactatatag taaatagaat
      601 tatgctatgg ggaaaactta ttgataatcc ttctagctca gatgtaagag ttatagctaa
      661 gaatgattct attattaaaa aagcacttga tagattaaaa gaattaggta gcaatgaaga
      721 atatttatta aatctgaaaa gaggtgctta tatcatgaat aaaagtagaa attttaaaga
      781 agagattgaa agagagacag caattagagt tgcaaaagaa aaagattata aaataattat
      841 aatgttgaaa aaaatggttt taaattagat gacatattga ataaatttaa tgattttgac
      901 ttaagtgaag atgagataaa agaagtatat gaaaataatt gatgataaat aaaaagtgaa
      961 cttatataag tttgctgaat ctgagcctgg aaatatttat gtgtattttt taatgaataa
     1021 aaatttattt taaaaacaat aattagaggt acagttaatt atatttttat gatactgtac
     1081 ttctagtttt ttctattgta gatttattat ttactaataa atctacaata gaagtgcttt
     1141 tgttgtaaga atcattttat ctaatgattg ttttttataa tagttaaatt tccatttgta
     1201 gtgcttaaat ataaatttgt aataccattt ttataagtgt ataaatcact atgacgattc
     1261 aactcaaagt cactgtccaa aacaccagaa accttatcaa aatttaaagt gaaacctgga
     1321 ttatcaggaa tagacaaatc gacattggca gaagtagttt cacagtacag agaagacaac
     1381 attttgctag aattgatttt tatattactg ctagttgact cagctttaat gtcagaaaaa
     1441 actccagata agttcatttt tccagaaact gaactacaag taagattttt attgtatata
     1501 tcttttaaat ttatattgcc attagttgtt tctaagttta aaacatcaat tttcccagaa
     1561 agctgtattt caccagatgt tgatttgagt gtagcatttt tactatttag ttttgttata
     1621 tcaatgtgac cactagttgt gtctaaattt attttattaa tattaccaga gagacctatt
     1681 gcacctgaaa ttgtgttagc ttcaactgta ttacttctta tgtaagtgct atatatatct
     1741 gctgaagtag tatataaggt tattttatca taaagcttat tgggcaaata tagttcaagt
     1801 attgttggtt ttggaagatt aattccaatg aagaattttt tagttgaatt atcatatata
     1861 gaaagagaat ttccgtcaac cattattttt gctagttttt tctttgagaa attactaggg
     1921 attttttgaa ttaatttgat tttattagaa tcacttctct taattctaac ttcacctgaa
     1981 atccaatcaa cattaatgtc atttatttta tctgaaaaac tttcattttt agccactata
     2041 tagttatcat aattgtattt aggattaaga gtattaaaag ggaaattaaa agaatgtgaa
     2101 attatagttc ctataaggag tgagataaat acaatagaaa gtgatattgc caggatactc
     2161 cagatgaaaa tctttgcctt tataagcttt ttattcatat ttatcctcct ccttgtagcc
     2221 ttttagttgt ataattcctt tgacaatgtt gacaccagca gcacctatta taaatagtat
     2281 ccacgaaaag gaccaaatat taaatagtat gcttgtgatt agatatatag aaactactat
     2341 aatccaatat gcattcataa atgatttata aactggattt atatgattat aattttcctc
     2401 aaaatcctca aattctttga atttatttga taaattatca ttgtatagag catagtttgt
     2461 ccttgtcatt tgattataga ctaataaacc tactccaagt gctattaata tgagcattaa
     2521 aagaatagct atagttggtt ctatgccaaa acttacaact gagactacaa ataggaaagg
     2581 gcttattata aaaagagcaa ctgctattga tgctcttaag gctcttttct ttttttcttc
     2641 aattaattca gaaggtagag aatatggttc cttaacactt tcaattaact cagataaatc
     2701 accaattcct gagattactg agttatatgc ttcttgttct gttttaccat cttgtatttg
     2761 gtcgttatac ttttctttta gattttcaaa taattcatct ttaagttctt tgactttttt
     2821 tgtctcatta actcctgtaa agctactgtc aatataagta cgtattttgt ctaacatatt
     2881 ttcacctcct aaattaatat atctatcaat gcttttgtat tattccattc ttcacggctt
     2941 tttatatatt tctctttacc tatgtttgtt attgaataat actttcttct agctccagta
     3001 acttcatcac cccaataaga ggttattgca ccagattgtt caagtctacg aaatgctgta
     3061 tacaaagtag cttctttcaa ctcatacaat cctttggttt tttcaagtat agatttatta
     3121 atctcataac cataactgtc tttttcaagt aaatgtttta agataattgt ttctgtatga
     3181 cctcttatca tgtcagatgc gattatcatg aaaatcacct ccaattaaat atataatact
     3241 ttgatatact tcgcctgtca aggtatatca tgagaaaaga cattttattg taatatttat
     3301 aaggttttat atagaaataa tgtaaaaact ttataaataa tatattttat acctagttat
     3361 agataacttt atatagttat tattaaaaaa agcaataaaa agtttaattc aatttagcaa
     3421 atttagtgat ataattaaaa aagaaaagaa atatggactt atttggaata attttgacaa
     3481 tctatttact acacgtgtag agtcgtatat aattaaatta tacataaaat ataaaaaaat
     3541 aatttagtaa tacgttagga gaaagtatta tgataaagtc aagcttattt tggaatattt
     3601 tcattgcgat atcctttatg atagaatggt ttctttgtaa gaaaatgcta gattatacaa
     3661 gtgagaaaag gatttctaac aaaaaaataa acttttgtat ggtaggtatt attgtttttg
     3721 ctgacataat atattttttt gatgtacctc caaatataag agtaattata tctatgataa
     3781 ttactattgc attttataaa atctcatatg atgtgggatt tataaagtct gtattaatat
     3841 ccttaattta ttggatgtta ctaatcgcca cagatgcttt gagtatatct ttaacagttt
     3901 ggcttaattc tataaatgac atgagtagta tggggacggg agatatatac agaattgagt
     3961 ctataatatt gggaaagata cttttgattg tgtctttgtt tttatataga gccttaaaga
     4021 taaaacttaa tatagaaata aataaaagga cattatttta tatagttatt cctatagtcg
     4081 ctaatatagc atcttttttt gtaatttttg aatacatatt taatccaggg tctggtagct
     4141 taatacataa tttccaattc gttatgattt ctgttgtatt gcttatatca agtatcactt
     4201 tagttttaat tgctaaaaaa ttacagcaat ataatacttt agttgtagaa aacaatatga
     4261 ttaaaaatac tttgaagtat gaaaatgagt attatgaaga tatagaaaag aaatatttaa
     4321 aaactagatt actaagtcat gatatgaaaa atcacatact gtgtataaat gctatgatta
     4381 aaaaaggtat tgatgttact tcttatttaa ataatttgca ggtagaaatt cagagtaatg
     4441 acttgttatt taatactggt aattttatat tagatgctat attaagtgaa aagaaagaag
     4501 tttgtgataa gaatgatata gaactaaaaa taggaataaa ttttagttgt tgtgaagctg
     4561 ttgaattagt agatatatgt catatatttt caaatatttt agacaatgca attgaggcat
     4621 gtaataaaat tgatagtgga aatagagtta tttgtgttaa aggtgatgtt atgcaaaatt
     4681 tttatttaat aagagttgaa aatactaaga tgaataagat tattactaaa aatggagata
     4741 ttttgacaga caaaaaaggt tctcttcatg gacttggaat aaaaagtgta aaaaactctg
     4801 taaataagta tggtggtaat gttgtaattg aatatgatga taaaaaattt gtagttaaaa
     4861 ttgctattcc acttagaaat gtgaagaaag cagttaatca tgtgatttaa atgtatataa
     4921 atttgatgtg tagaaattta atatataaga attaatgta
//
LOCUS       DAELSY010000216         4908 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.217, whole genome shotgun sequence.
ACCESSION   DAELSY010000216 DAELSY010000000
VERSION     DAELSY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.217"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(532..1173)
                     /locus_tag="KPG09_003580"
     CDS             complement(532..1173)
                     /locus_tag="KPG09_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBG0968138.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
     gene            complement(1416..2219)
                     /locus_tag="KPG09_003581"
     CDS             complement(1416..2219)
                     /locus_tag="KPG09_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0968139.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKDKNEVIKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGIEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            complement(2375..3550)
                     /gene="thiI"
                     /locus_tag="KPG09_003582"
     CDS             complement(2375..3550)
                     /gene="thiI"
                     /locus_tag="KPG09_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBG0968140.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            complement(3596..4747)
                     /locus_tag="KPG09_003583"
     CDS             complement(3596..4747)
                     /locus_tag="KPG09_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBG0968141.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDVVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
BASE COUNT         1524 a          929 c          465 g         1990 t
ORIGIN      
        1 tacttttcta aaaattatct atgttaatag ttaaatttat gaacattttt tgtttttatg
       61 ttaggatatt tcttagaaat gaaatacttc ttaatttgat ttgatatgta tatttttata
      121 gctattttat tataaaaaaa aagaaccctt ttacaactaa gggttacttt ttttctctat
      181 tttttttatt atatcactat tttttcccta tttcaatatt ttgacatttt ttccattata
      241 ctgaaaagat atcttaaatt tttaacttgg ataaatttta aagcatatac ttaaaattaa
      301 catacttgtt ttctatttta ttacacttaa tccttatttt tatcattcta caaaacaaaa
      361 ataaaaaaga ataagataat aaataaaata cttatcacct tattctttat ttaattattt
      421 tacaatgaaa aatctaaact actttttaaa tagcttcctc ttccatcaat ttatttgtat
      481 attactggtt taattcttca agtaactgat tggttcttgc aatactttat cctaaactct
      541 ggaaactttg taaactacct ctctagcatt tggtaaattt ccatctggaa tacctacagc
      601 aataccaaat ggtacagaat acccttctgg tatatcaagt agttctctgt attttttatt
      661 tgtatcaaac ttcatcttta ttgactctga ttcctttaca tcttctttat ccaatacact
      721 cacaagacct tcaatatccc cttgttcaac aggtgtaaat aaacctctaa ccattcctat
      781 tatacaactt ccaagcccta gtgcttgtgc agctgttgtt atattccctg taagtattcc
      841 tatatcatat ggagcccatg aactactttc atcataagaa ataaataaaa cggttggagc
      901 accataaaat aacttaaagt ttttatttat ctcaacatta ctcacttcat gaatcctaga
      961 taaagtatca tcactaatct cttttaaaag tttctggtct tgcacaacag ttatgtgcca
     1021 aggctgttta ttacatcctg aaggtgctgc aaatgcacag tgtaataatg tgtttacttg
     1081 ctcatcacta atttgctctg ttgtaaattt tcgtatactt ctacgacttt gtattaaatt
     1141 tattgtttgg ttgtcttgaa aattattatt catatctatt ccccttttta tgtatttctt
     1201 agtacaaata ctaaatcttc agaaaattaa tcttataatt aaatttcttt acaatattat
     1261 tataattatc tttagcttgt atgtgaagac ttaaaaatat aaatactttt aatatatctt
     1321 aattatacat ataaatgaaa atctggtatt cgtcatctat agaaactgct cttttctata
     1381 gtttaatcaa taccagatta ataaaacatg atatattaat ttaaaggacc ccatccctct
     1441 ttagaatgaa tctctgtttt tacagactta acactatctg tgtcaacatc ccatgaccta
     1501 cctgcaacag aaataccatt tttaccttta taccatctac cttcatcata atcgtaaact
     1561 agggaagtat accaattacc tccagtctta ttatttgcaa atacaactac agtagcatca
     1621 gatttagtag gattatatgc atatggttgt atatatagtg tatccttact taatccatat
     1681 tttgccttaa attcatctgg aattgtaggc cacccatttt tatatttatt gtctaataaa
     1741 tattttctaa aatcatcatt gccctttgaa gctcctggta ttatagaagg gtcttgtgca
     1801 aatgatgcaa tcaaatcttt catactttga tgtatatccc tagccatttc aataccatct
     1861 ggatgttttg tacaagtaac atacactttt gcaatgcttt ctattccagt tattccatca
     1921 taaccatcat caaacgttgc tgaatatatt ccacctgact tacactttgg ttctgtttca
     1981 aagtacttac catctttgtt ttctaatacc tcttttatga cttcattctt gtctttactt
     2041 gattcctcag ccattgcaat aacaatttga gtctttatat tttctctatt agaaagacat
     2101 gtaactgcct tacttttttc tatatttcta aataacgctg gaacagctac tataactaaa
     2161 attcctatta tagatataac taccaacaat tcaattaatg taaaaccctt tttattcata
     2221 ataatcattc ccattaaata aaattatagt acttattttt aaatactata tattaataaa
     2281 ttgtctacat aaatcctttc aacagaacta ctaatatact tacagaacta ctaatatact
     2341 caatattcaa tttagtaatt aaataacttg tatgctaaaa ctcaatatcc tctacctcta
     2401 ttttgtcaat agcatcctgt actaattttt caatatcaag tgctgtttca gatttttcta
     2461 ttttctctaa tctaggttta gtgacaggct ttctaggaga aaatacacta caacaatctt
     2521 cttcaggcac tatagatgtt tcaaaagtcc ctattttttt ggctatatca actatatctg
     2581 atttatccat tgctataagt ggtctaaaca ctggtaagtc tacaacggca ttagtacaag
     2641 taagcccttg tatagtttga gatgcaacct gcccaatact ttcacctgta attaaagcat
     2701 cacaatgtct tttttcagaa agtctctgtg ctatcctcat cataaatctt cttgaaagaa
     2761 tagtagcttc ttcttcatta caattctcac caattgcttt ttgtatttct aatatgttaa
     2821 ctttatgaag tcttactcta ccacagtatt ttgccagtat tttagctaaa tccttaactt
     2881 tttcctgtga cctttcactt gtaaatggat aactatggaa gtgaactgcc tctacctcca
     2941 tacctctttt tgcaaccatc catgtagcta ctggactatc tatacctcct gataaaagag
     3001 acatagctct accattcgtt ccaagtggca atccaccata tccaggtata gtatcactat
     3061 agaccatagt atgaaattct ctgtattcgc actttatttt aacttctgga tttcttacat
     3121 caacattaat cctatctcct acctttgata ataagtatcc acctatatcc atactcattt
     3181 cttgagaagt caacccgaaa gacttatctc ctcttcttga ctctacctta aaagttttat
     3241 atccagcctc tattttttct tctaacattt ttaaagctat ttcttttaat gtatcataat
     3301 cttttttagc tcttactcca gggcacacac ccactatccc aaatactttt ctaacttctt
     3361 caattacttc ttcataatca tagtcttcta aatcaacata tattcttcca tactctttat
     3421 atacattaaa tcttccaatt ggttttaaca tatttcttat atttcttata agcctattct
     3481 caaatatata tctattcttt ccttttactc caatttctcc atactttact attaatatat
     3541 tatacaccat ttcacctctt aaactaattt attatcacta tctatacagt attctttatt
     3601 ttcttttcat tattgacctt aagtcacaaa ttgattcttt caaaactttc acagcttcta
     3661 acatttcttc ttctgtattc atatcagata aactaaatct tattgttcca tttatttctt
     3721 catttgtaag acctatagca tttaatacgt aactaccttt ctttttagaa gaacatgcag
     3781 agccagttga tacataaata cctttttgct ctaggtaatg aagtaaaact tctccttttg
     3841 tccctctaaa tgatacattt aatatatgac atactccatc ttctggagaa ttgattttta
     3901 tatcttctat attatcaatt atttcatttt ttaatatatt ttttaagtta tttactttcg
     3961 aaataacaac atctaaatct ttgtttagta ttctaacagc ttctccaaga ccatatattc
     4021 caggtacatt ttctgttccc gacctaattc caatttcctg accaccacct gttaacatag
     4081 gcttaaggcg attattttct tttatataca taaatccaat tccctttgga ccatgaaatt
     4141 tatgtgcact tacactcata aaatctatat tgtatttaga tggtctaaaa ttaatttttc
     4201 catatgactg aatagcatca acatggaaat aaattttttc ttttaaaccc ttcaaatact
     4261 ttccaacttc acctattggc tgtatagttc ctatttcatt gttaacatgc atcatgctta
     4321 ctaagcatgt agttgacttt atcgcatttt ttaaatcctc tatatttatt ttcccatctt
     4381 ttccgacctc taaataagtt acttcaaaac catcttcttc taaatctttt aatgtattta
     4441 aaacagaagg atgttctatg ttagtagata ttatgtgatt ttttcttttt ttatttaaac
     4501 tagtaatacc tcttattata gtattattac actctgtacc acctgatgta aaataaatct
     4561 ccttttcttt cgctcctaga gatttagcta tatcttgtct tacttctttt atagcttttt
     4621 caacttctac accttttcta tggacagaag aaggatttcc atattccgta ttaagtgcat
     4681 aaaccatttt atctataact tcttgataag gttttgtagt tgcgctattg tctaagtata
     4741 tttccaactt aattacacct acctttattt aaaatcttca actctaacta ttataccact
     4801 aattatagta ttttttttgt taatctttat taaaaagaca atcttaaatt ataaaatatt
     4861 atttcatgaa aagctagttt tttcacttta ttacaaattt ttatagta
//
LOCUS       DAELSY010000217         4889 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.218, whole genome shotgun sequence.
ACCESSION   DAELSY010000217 DAELSY010000000
VERSION     DAELSY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.218"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(64..828)
                     /locus_tag="KPG09_003584"
     CDS             complement(64..828)
                     /locus_tag="KPG09_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG0968142.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKAPTPDIVDILSEFNVNVL"
     gene            1102..1776
                     /gene="fsa"
                     /locus_tag="KPG09_003585"
     CDS             1102..1776
                     /gene="fsa"
                     /locus_tag="KPG09_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG0968143.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILDEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(1898..3292)
                     /locus_tag="KPG09_003586"
     CDS             complement(1898..3292)
                     /locus_tag="KPG09_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBG0968144.1"
                     /translation="MGIILKPKQFYMTVIVTIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(3370..4716)
                     /locus_tag="KPG09_003587"
     CDS             complement(3370..4716)
                     /locus_tag="KPG09_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0968145.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGIQQMLFSIGNMTLQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGYWKTKAIVNHRINE"
BASE COUNT         1909 a          680 c          627 g         1673 t
ORIGIN      
        1 taagaatagt tatcaaataa aaatttatat ttataagaaa tcctataaaa tatataatta
       61 aacttataaa acatttacat taaattctga taaaatatct actatatcag gagtaggcgc
      121 tttttctgta attatagaac taatatcttc taaatgagca aacttgaaat ttccatcaaa
      181 tttaaacttt ttatcttcca taactataaa tactttttta cttgaattta ttatagcctt
      241 cttcgtgaga ccatcttcaa tttcaaaagt tgtaagtgaa gagttgaata catctactcc
      301 acaacttccc ataaaagtct tatcaaactt atatttcgat ataaactcta tagttggagt
      361 accgacaaaa ccgtctaaac tgagatttaa taaacctcca gtggaaatta tatttaaatt
      421 atttggtttt gaagtaacta catttagtat atccaacata tttgtaacaa gtgttacttt
      481 tttattactt tcagctaaca actttgcaag caagatgtta attgtagaga tatctaaaaa
      541 tatagtttct ctatcttcaa ttaaattaaa tgctttttct gctattattt tctttgttgg
      601 aatattaatt tcttttctgt cttttgtatc ctgattctct agtgtttctc tagctagtac
      661 agctccccca taaattcttc ttattgaaga aatcttttct atatttttta aatcttttct
      721 tatacaatct tctgtaacat taaatttaga actgagctct ttgacagtaa ctctaccttc
      781 tttttttagg atatttaata tagcttggat tctttcttct gcaaacattt tctacctctt
      841 ttcgcaatat agtattatag taaatattat aagataaaat acaatatatc aactatttta
      901 acatgaaata caaaattaat ttaatataaa aagtatgttt tttctaacta tatatttaga
      961 atttagtaaa taaaaaaatt ataaaactaa gaaggaaaag caaaacaata ataaaaaata
     1021 aaaaacaata agcaaaatgt tgaaaataat accaaatcaa tatataataa gaataaagaa
     1081 agattaaagg gggatatttt aatgcaacta atcttagata caggtaatgt ggaggagatt
     1141 aaggagttat gtacttgttt accaatagat ggtgtaacta caaatccaag tattgtttca
     1201 aaagaaaaaa agaattttaa gcaacttata aatgaaattg gtgagattat aggtgaggat
     1261 atgcctatac atgctcaagt gttaagtaca aagtatgaag aaatattaga cgaagcatta
     1321 tatataagta gtttgagaaa aaatatatat gtaaaaatac ctgtaacaca agatggttta
     1381 agagctataa aagaccttca cagaaaaggt gtaaaaataa ctgctactgc aatattcact
     1441 gctcatcaag gttttttagc tgcaaaggct ggagcaaatt atattgcacc ttatgtaaat
     1501 aggcttgata atatatctgg tgatggagta gctatggttt cagaacttat aaaaataata
     1561 gatacttata aaatggatac aaaagtactt gcagccagtt ttaaaaatgc acaacaagtt
     1621 atagaattaa tgcaacatgg ggtgcatagt gttacagtac catatgatat ttgtaaatct
     1681 atgatgaatc atccattaac tgattggagt gtagataagt ttatagagga ttgggagaat
     1741 acttttggaa aaggaagtaa aacaaataat atttagtatt atggtaaaag agtagttttg
     1801 tccaatttca caagaactta tataaaaaga tgaaaatatg aattttcttt ttaataattt
     1861 cgttattatc ttaagttaat tcatattttc atctttttta gatttctagt cctataaatt
     1921 tccaccaagg gaatgttata catattatta taaagtaggt taaaggaatc aaagttaaac
     1981 cgtatttgaa aataaccttg ctattataca gattttcacc atgaccaacc attattgtag
     2041 catgatgaaa tggtaatatg tactgtatgc ttactataat gtagacaaat agacataaag
     2101 taacactatt ttcctttagt atatgtaaac ttccaagtaa tggtattact acagatgatg
     2161 tagtgacagc acttccaaga catatattca agattattgt gagagttatc aaaaacaata
     2221 ttgaaataat ttctgaagag tttggtataa actttatcaa ataatttccc attatatcta
     2281 caactccaga gtactttaac actcctccaa ttgaaaatgc tgctataaaa aaaataagga
     2341 ccttccaatt tatttctttt aaagtactaa atccaactat ttttcttagt aataaaagaa
     2401 gtacacaaat tgacattata attaatgtgt ttatgttatg tatgaactga gttaaaaaca
     2461 taaaaaacat tattcctagg cataaaaata cgtgtttttg ctctttgttt agcaaattaa
     2521 attctttttt taaattagaa tatgaaaaat gaagtttatt accctcaaga ttttcattag
     2581 aaataaattt tattgaatgc aattcttttt taaagacaaa gataaataaa aaataagtta
     2641 taaaacaagt aattatagtt ggaacagaca tataaaaagc ccattgaccc caatttatat
     2701 tacaatttcc aagttctagt acaacataat ttagaagggt gtctccattt acaaaaaaca
     2761 ttgatgtaaa ggtagatgct gtaaatatac taaataaaag tatagatttt gaatcttcag
     2821 ttatctgttg ttcttttaaa aactccttgt aaaaagcaga tacaagaata acccttggaa
     2881 atggttgtgg gataaaaaat attaaaagaa tacctaaaat atacgataaa agaatcatct
     2941 ttttaggggt atttacagta tttaaaatta acttctcaga aataattttt gcgacacctg
     3001 tttttgtaac tgcattagta attatgtatg caagaattat tatgtaaaaa ttttctgtaa
     3061 ataaaaactg taaaataacc tttattggtg caatgttaaa tataaacgct agaattacaa
     3121 aaaacaatgc aacaaaagtt ttttcaactg agtctacagc ccatatcaag atagttaaaa
     3181 aagataagca tatagaaaat tttatgttga aactgtaatt aagagagaag tagagaatta
     3241 tggtaactat aacagtcata taaaattgtt ttggcttaag aataataccc atttttatat
     3301 tcacctccat aatttattat atcataatgg aggtgaatac aatgaatgtg aatacatttt
     3361 ataaaaagat tattcattga ttctatgatt tacaatagct ttagttttcc aatacccttt
     3421 cttatagtag ataactgtaa caataagacc taaagtccaa ccaaaaggaa tacccatcca
     3481 aattccgtct gttccaaaaa atttagaaag catataagaa gctggaagtc ttccaagcca
     3541 taaagatact attgagctta taagtgcaaa catggaatca ccagcacctt tcattgcact
     3601 tgaaagtaca aaagaagttc caataaataa aaagaatggt cctattataa ataagtaaga
     3661 agagccaatt ttaatgacat ctttatcagt attgaataga gcaattagat tttccctaaa
     3721 tagcaaaatt aaagcaataa ctgtaactgc taaaactaag gtcattttta tagattctcg
     3781 aatgcctttt ttaacccttt cattttctcc agcacctatg ttttgagcaa caaaagtaga
     3841 aacagcagaa ccaagattca taataggtaa gcttataaaa ttttctatcc taagaccagc
     3901 accaaaagct gccatagcag atgtaccata actattaact aaaaattgaa gagtcatatt
     3961 tccaattgaa aacaacattt gttgaattcc acttggaagt cctaatctaa gtccatcttt
     4021 aaatattttg ttatcgtata caattccttt gattttaaat ttaagcactt catgtttttt
     4081 atttaaataa attatactta ttataaatga tactgcctga gccattatag tggcaagtgc
     4141 aacgccctca acacccattc ttagcactac aataaaagtt aagtctaaaa ctatatttag
     4201 aattgtggct attatcagaa aataaagtgg agttactgaa tctccaagtc ctctaagtat
     4261 tgcacttact gaattatatc cagcagaaaa taatattcca ctaaatatta tagtaagata
     4321 caattttgat tgagcaaaaa cggattctgg tgtattcata agcttaagca ttgggccacc
     4381 aaatactatt ccaagaatac ttattaatat agaaccaata aataaaagta tatatgtagt
     4441 atcaatagta gcttttacct tttttaaatc tcctgagcca taaaattgtg acactaaaat
     4501 agaaaatcca aggctaacac ccataagcgc tgccaccaat aaaaacatta tagggttagc
     4561 tccagagact gccgccattg cttcttttcc gataaatcta ccaactatta tactgtcagc
     4621 tgtattgtat agttgttgaa ataaactacc aataagcata ggcatggcaa aaaagaatat
     4681 ggattttccc tcatgacctg tagttaaatc tttcacctta ttcctccaaa taaaaaaatt
     4741 attaatttat tatagtataa tcttaacata aactttatat ccaaactata aataaattgt
     4801 atcaaaatat catatatttt tttaaaatgt aaagaatata attataattt gaatttaata
     4861 aatcaatatt taatataatc gaatattaa
//
LOCUS       DAELSY010000218         4846 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.219, whole genome shotgun sequence.
ACCESSION   DAELSY010000218 DAELSY010000000
VERSION     DAELSY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.219"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(175..660)
                     /locus_tag="KPG09_003588"
     CDS             complement(175..660)
                     /locus_tag="KPG09_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG0968146.1"
                     /translation="MISELMLKNFGKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     regulatory      complement(743..836)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1192..1575)
                     /locus_tag="KPG09_003589"
                     /pseudo
     CDS             complement(1192..1575)
                     /locus_tag="KPG09_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      1802..1899
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1954..2130
                     /locus_tag="KPG09_003590"
     CDS             1954..2130
                     /locus_tag="KPG09_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968147.1"
                     /translation="MFGNKMDKCTHVLTAYISGSYDYCNFLDTQLDDFILEYGESVVE
                     SCLHQVMVLVSKYN"
     gene            2356..2556
                     /locus_tag="KPG09_003591"
     CDS             2356..2556
                     /locus_tag="KPG09_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG0968148.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            complement(2720..3019)
                     /locus_tag="KPG09_003592"
     CDS             complement(2720..3019)
                     /locus_tag="KPG09_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968149.1"
                     /translation="MGFYINYVVCKVKILFSSFSKIILFYINYVVCKLFITLKAILLK
                     TQIIVTTHSPHILQIDSKEEMIVLDMAESGNVYKKELELGEYGVLGWTNEGLYLH"
     gene            3241..3423
                     /locus_tag="KPG09_003593"
     CDS             3241..3423
                     /locus_tag="KPG09_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBG0968150.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIVF"
     gene            3494..4339
                     /locus_tag="KPG09_003594"
     CDS             3494..4339
                     /locus_tag="KPG09_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968151.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINNSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
     gene            complement(4563..4727)
                     /locus_tag="KPG09_003595"
     CDS             complement(4563..4727)
                     /locus_tag="KPG09_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968152.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT         1779 a          627 c          528 g         1912 t
ORIGIN      
        1 tataagttaa atctactttc aattaaattt accatttata attactttat aataaaaata
       61 aattagttaa tttattctct ttagttctgt tataatgttt ctacaaaaaa gaatcccatt
      121 aatgggattc ttttttgtga tttattaaca tatttaaagc tcactagtat cgtattataa
      181 acttaaatgc tcttctatgt attgattata tacatatcca gaatatcgtt tttcattttt
      241 attgatatta actcttgccc attttccaac gctataacat acttcaactt taaaaccatc
      301 agggattgta gttataactt tagattctaa tgttctatca tttctaagtt ctaaacttaa
      361 tccatttgtt cttatatttc ctatgtaatt cttttttcta ttttttattg tatcattact
      421 attgttttta gcattactta atctttctaa atatttataa agtaaccagc ctttgtcata
      481 attactctca acttctgccc atattccatc tacacatttt aacttaacaa ttttaccttc
      541 tggtatactt tttaatttca aactatttat gtctggtttt tctcttaaat tcaatttaat
      601 tttagtttta aaattgatgc tagatttgcc aaaatttttt aacattaatt cagatatcat
      661 cttaagccct cctcttgcca aatattaagt tattaaaata tataactcta aaaatacttt
      721 taacaaaaag aattattatc tttttggcaa ctggctgaca tgacctttat tacataaaga
      781 ttttagtccc tatagttttg cgtcattaga tttatctaat attgcctata tctgaataat
      841 aacataattt gaacaattaa aaaataatat tacctcaaat tttatattat ttaaatataa
      901 attaaatttt taattaataa taatcaaaaa atatctttat atttattcaa ttactgttct
      961 ttatagtcaa tataaaagac agtaatttat ctatagtgtt acttatattt tatatatatg
     1021 gctatttttc aacattttac aatatatata acatttattt ttttaacttt tgtcagtaaa
     1081 atgtcagcaa gacactttat ccacaatttt tatataagtt ttccacaact tatccccagt
     1141 attatccaca taaaaaaggt agctcttata ttattagcta ccttttattt attaatcttc
     1201 atatttccag ctttttatcc aacttcctat ttctattatt ttttcttcag ttatttcttc
     1261 tccatcatgt aattttgaaa ttaattctga taaaggattg ttatgtaaac ctctaaaaac
     1321 tctttttccc tctattttga gatattctct ctcttttatt aatactgtat agtgtacatg
     1381 cctcccaatt ctctatgata ttaaaaaacc ttttttggtt aatctagcta aaagtataat
     1441 tgtagttgta tgcttccaac catatgtttt ttctatttca tctgcaacat cttttgatat
     1501 tacagtttta taaccagtat tccatatgca tttcataatt tttaattctg tatctggtaa
     1561 ttttttattt agcataatat attccttctt tcaatttatg tcacttcttc aaatttatct
     1621 agcttaaatt ataataggag ttttttagaa caaaaactat agttaatttt taccaggtac
     1681 tctacgtgtt tagtctgttt tttaagtgta aaataatgtt catatctata aatttattta
     1741 actttcaaac aatcctgttg aataataact aattctattg tattatttta gttgtagaat
     1801 aaaactaaat cggcaaaact agagaaattt agtgacgcaa agctataggg actaagactt
     1861 atataaatat cttatgagtt atgtcagcca gttgccaaag agatagtgtt ctttttgttt
     1921 ttatgaaagt tttttagggg gataataatt tatatgtttg gaaataaaat ggataaatgt
     1981 acacatgtat tgactgctta tattagcggt tcgtatgatt attgtaattt tttagataca
     2041 caattagatg attttatatt agagtacgga gaaagtgtag tagagtcttg tttgcaccaa
     2101 gtgatggtat tggtaagtaa gtataattag atagacattg atttctagag aacccttttt
     2161 agttttatct acatttatat taatatatag caatgtaaaa cgcttaaata tatttttaat
     2221 attaagaata actaaaatgt tgactaaaaa atataattaa gtagacattt ctattaatct
     2281 atgatacaat agtatttgtg gtaatacaca attaaatcaa gtatatcttg atataggtta
     2341 ataggagtat ttttaatgaa taatggaata gtgaaatggt ttaataatga aaaaggattc
     2401 ggttttatat caatggaagg tagagatgat gtgtttgctc atttctcagc tatacaaact
     2461 tcaggattta aatcattaga agaaggtcaa caagtaagct ttgatatagt taaaggtgct
     2521 agaggtcctc aagcagaaaa tataactata ttataatata tagatttttc gggaaagact
     2581 ctttttagag tcttttttta ttgttagtat atgtaagata atcattggtg aagtaaataa
     2641 ggtatattga gtaatgttct tgatagttta tatgctaatg aatttcaaaa aaataagcac
     2701 tcctataaaa agagtgctct taatgtaaat ataagccttc attggtccag cctaataccc
     2761 cgtattctcc aagttctaac tcttttttat atacattacc actttcagcc atatctaaca
     2821 caatcatttc ttctttagaa tcaatttgta aaatatgagg actgtgtgtt gtgacaatta
     2881 tttgtgtttt taggagaatt gcttttaatg taataaaaag tttacataca acatagttaa
     2941 tataaaacaa aattattttt gagaatgagc taaataaaat cttaacttta cataccacat
     3001 agttgatata aaaccccaaa ataaacttag catctccaat gtttacacat atacaatgct
     3061 cttaaaattg cagtgaacat gcaatagtgc aattgataat acttatcata tattctcaat
     3121 accttatatt tcaactgcta aacctcaatt tgtcacaaat atcgctcact gcaaaatttc
     3181 tatattttta ttatatcata gcatataatt aatattttca attaattttt acatttaatt
     3241 atgtttaaat tctataataa tgcaacttta atgtttcttt ctggaactaa tataaaaact
     3301 atttcagaaa gattaggtca tgcagatata aaaataacta tgaatagcta ctctcagttt
     3361 ttagaagaga tggacagaga agcatttgaa aatttgagca aaatactatt tattgtattt
     3421 taaagaagat gacttattca atatttgatg ctagaatata attaataaaa atttaattat
     3481 aggtgattgt attatgaaca ttattaaaag cataacttta ctcttaacat tattaattgt
     3541 gatattaatg acaggatgtt ccccaagaga aagtgctagt gaagttgcaa aaaaatatat
     3601 tgataagatg aactctggtg actatgaaac agcatatagt ttattaagta aagatagtaa
     3661 aaaaaaaatt agtatagaaa atcttgaaga atttcaaaat attttagtta gtacaaaaag
     3721 aataattagt tataaaatag gtaaagaaaa aatcataaaa aaatataaac atgatggaaa
     3781 agaatataaa aatgttgtta aattagaaga aacttttaat ataaaaaacc ttctttataa
     3841 aaaagatgat tctctaaaag taaatagata ttttgtagtt gaaaataaca aatacaagct
     3901 attattgtat gaaagtttca aaaaagaatt tggtattaat agtatggacc ttgctcaact
     3961 taagcttaat gattttggac taaaatactt taaagaagtt gaccttatac taaaaaaagc
     4021 tattgctatt aatcctactg attcaaagct ttattatgcc caagcatgta attatatgaa
     4081 ttttgatgga tttcctggtt attcagatac aacatataat gctttagatt caattaataa
     4141 ttctttaaag tatttagata aaaatgattc tgactacaaa aaaaatgcaa gtaatatata
     4201 taacttaaaa ggagttattc ttatgtttca caaaagattt gatgaagcac aacaatattt
     4261 aaataaagcc ttatacttta acaaagataa tgaagaccca aaatataatc taaatacaat
     4321 aaaaaaacat ctaaactaga tatttaattt ttgaatttat aaaatatttt tttatgtata
     4381 attcagcact gaatcataat ctctactaaa ctaattacaa gtttaaatta taaagactta
     4441 tttctatata aatattactc tataacaata agtttcttat ttaaatttta aaatattatc
     4501 taaaaatcat aaaaaagaag gtataaagag ctaccctcaa gctcaatata ccttataaat
     4561 atctaaactt cattttttac taatttttct atatctttga taatatgttc tttagatttt
     4621 tttccagcta tggcatcaaa aattgcaaca gcctttttct ctccaaactt ttctgctaca
     4681 tcattaaagt ctaaaaatct aacactttct tttggtttac caaacatata acacctctca
     4741 tacattttta tatatttatt ataacttcta tagacttatt tttcaccaat aaacgttaga
     4801 caaattagtc cctttatttt tttaaactag ctttaccttc aaaatt
//
LOCUS       DAELSY010000219         4727 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.221, whole genome shotgun sequence.
ACCESSION   DAELSY010000219 DAELSY010000000
VERSION     DAELSY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.221"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            284..1576
                     /gene="eno"
                     /locus_tag="KPG09_003596"
     CDS             284..1576
                     /gene="eno"
                     /locus_tag="KPG09_003596"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBG0968153.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTERLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
     gene            complement(1696..2124)
                     /locus_tag="KPG09_003597"
     CDS             complement(1696..2124)
                     /locus_tag="KPG09_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0968154.1"
                     /translation="MNPVKELNNLWHIVSMQMKTEMCSGKYPTIHGISLVELSILEIV
                     EQYPNSMMKEISEILELPKSTLTSAVKRLELKKFIKKNPCIKDKRRYNLVLTELGLKA
                     QQEHRDIEIIVFRNLLHQLDKDEIETFIRIFSKAVNKSKK"
     gene            2209..2715
                     /locus_tag="KPG09_003598"
     CDS             2209..2715
                     /locus_tag="KPG09_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0968155.1"
                     /translation="MNLEIIRATSENAIEMGFIHSCSWRKAYKGIIPDKIIDEFTSEK
                     RAEVFQSVIPKAEEEYYLFKVNGIPAGFASLNRSQEDNAPKCVGEIYSIYFHPDFWGT
                     SVTKKGLQFCIDRLSNLGYSYITIWVLKDNTRAINFYKKNGFTCDDFEKEIHIGKKLL
                     EVRYSKKI"
     gene            2833..3057
                     /gene="secG"
                     /locus_tag="KPG09_003599"
     CDS             2833..3057
                     /gene="secG"
                     /locus_tag="KPG09_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBG0968156.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(3441..4379)
                     /locus_tag="KPG09_003600"
     CDS             complement(3441..4379)
                     /locus_tag="KPG09_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG0968157.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIRH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKTIILGVCGKLILVPSIFLPISIFLGFRNVELTSLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(4367..>4727)
                     /locus_tag="KPG09_003601"
     CDS             complement(4367..>4727)
                     /locus_tag="KPG09_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0968158.1"
                     /translation="NIENYINFYKKFGFSSKEMSEKYKLKARNVKINLNIKGYKFLPY
                     VKNLKASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFGNMKSIYLKKTNGN
                     LIELSNHRITESKPWKI"
BASE COUNT         1784 a          565 c          758 g         1620 t
ORIGIN      
        1 agttgtacat tttatgttta atgttttaaa tctagttata atatttgtgt atttaagatt
       61 ggttttggat tttagacaag tttttttata ttatataaat taaaatatta taaaaaataa
      121 aataaaaata ggaactttta caaatttacc catcaaataa atttgtacct aattttcaaa
      181 agcaacttta tgtactataa tatagaaagt agattaaaat agtagacgga tgtttacagt
      241 aaatgaaaat ttaactaata gaaaatcgaa gggagatata caaatgtcag ttattgaatt
      301 agtatatgct agagaagtat tagactcaag aggaaatcca actgtagaag ttgaagtagt
      361 attagaagat ggagcaatgg gaagagcaat agttccatca ggagcatcta caggagcttt
      421 tgaagctgtt gaattaagag atggagataa gggaagatac ttaggaaaag gtgtagaaac
      481 agctgttgct aacgtaaatg aaataatagc acctgaaata gaaggaatgg atgcatttga
      541 ccaaccagct atagatgcaa tcatgataga attagatgga actccaaaca aaggtaaatt
      601 aggagctaat gctatattag gtgtttcaat ggcagtagct agagcagctg ctgatgaaat
      661 aggtttacca ttattccaat atttaggtgg agtaaatgct aaacaattac ctgttccaat
      721 gatgaacata ttaaacggtg gagagcatgc tgataataac gttgatgttc aagagttcat
      781 gatattacca gtaggagctt gttgctttaa agaaggttta agaatgggag cagaagtatt
      841 ccattcatta aagaaagttt taggagaaaa aggattagct tgtggtgttg gtgacgaagg
      901 tggatttgct ccaaacttag gttcaaacag agaagcttta gaattaatag ttgaagctat
      961 aacaaaagct ggatataagc caggtgaaga tgttatgtta ggacttgacg ttgctgctac
     1021 agaaatgtac aacaaagaaa ctaaaaaata cgttttagca ggagaaggaa aagagttaac
     1081 tgctgctgaa atggttgctt tatatgaaga ttggtcaaac aacttcccaa taataactat
     1141 agaagatggt ttagacgaag aagattggga cggatggaag ttattaactg aaagattagg
     1201 aaataaatta caattagttg gtgacgactt atttgttact aacacagaga gattagaaaa
     1261 aggaattgaa aatggagtag ctaactctat attagttaaa gttaatcaaa taggtacaat
     1321 aactgaaact ttagatgcta tagaaatggc taagagagca ggatatactg ctgttatatc
     1381 tcacagatca ggagaaactg aagattctac tatagcagat ttagctgttg cagtaaatgc
     1441 tggtcaaata aaaactggtg ctccatcaag aactgataga gttgctaagt acaaccaatt
     1501 attaagaata gaagaaatgg ttggagaaca agctagatac tgtggattaa aatctttcta
     1561 taatttaaaa aaatagttat tgaagattaa aaatatggca ttaaagatta aaaagataac
     1621 ataaaagata aaaatttatc ttataaggga taagtgatag ttgatttttc aattcactta
     1681 tccttttttt atatttcatt tcttactttt attcacagct ttagaaaaaa tacgaataaa
     1741 agtttctatt tcatctttat ctaattgatg taagagattt ctaaatacaa ttatttctat
     1801 gtctctatgt tcttgttgag cctttaaacc taattcagtt aagactaagt tatacctcct
     1861 tttgtcttta atacagggat tctttttaat aaattttttt aattcaagac gttttacagc
     1921 acttgttaat gtgctttttg gcaattctaa aatttcacta atttctttca tcatactatt
     1981 gggatactgt tcaactattt ctaaaatact taattctact aaagaaatac catgaatagt
     2041 aggatactta ccactacaca tttctgtttt catctgcata ctaacaatat gccacaggtt
     2101 gtttagctct tttacaggat tcattgactt ttacctccat atatgataca attatcgcac
     2161 taatatagta cgataatcgt attaatttgt caataggatg gtgtttttat gaatttagag
     2221 attatacgag ctacaagtga aaatgctata gaaatgggtt ttatacattc ttgttcgtgg
     2281 agaaaagcgt ataaaggaat tattccagat aagataatag atgaattcac ttctgagaaa
     2341 agagcagaag tttttcaaag tgttattcca aaagctgaag aggaatatta tctttttaaa
     2401 gtgaatggta tacctgctgg ttttgcttca ttaaatagaa gtcaagagga taatgctcca
     2461 aaatgtgttg gagaaatata ttctatatat ttccatcccg atttttgggg aacatctgtg
     2521 acaaaaaaag gattacaatt ttgcattgat agattaagca atttaggata ttcatatata
     2581 acgatatggg ttctaaaaga caatactaga gctataaatt tctataaaaa aaatggtttt
     2641 acttgtgatg attttgagaa agagatacat attggaaaaa aattattaga agtacgttat
     2701 tcaaaaaaga tatagtaatt gcatattatt tttgtttgtt atttatactt caatagtctt
     2761 gaaatgacta tattattgtg atacactaaa gagtaatgaa atttaagtaa taaatagata
     2821 ggaggggcaa taatgagcaa cattttaatg ggtgtgcaag tagtgctagg gattttacta
     2881 gtaattagca taatgcctca agattcaaaa aatgctttac cttcagaatt tggtggagaa
     2941 ggaagtcaag cttactttaa accaaaagga aaacaagcat ttcttgctag agttacaaag
     3001 ataacagcag tattattttt tataaatgca ttggcattac ttatagttaa gaagtagtga
     3061 tattgttgaa taactggcca ttatttaatt agtgcatttg ctagatattt aaaaattaaa
     3121 tttagttttt aataaaagta catcaacata aaaagtacgt aaaataaaag ctggactaat
     3181 gattttatat gattagtcca gtttttattt tatagattga ctatttgttg gcatttatta
     3241 tttgtattta tatattgttt atatttatat atttttttca aactaaaaaa tatatagcaa
     3301 cactactttc accatgttta atgagataat atacacaaag ttatattata tagtaaaaat
     3361 agtataataa tatgttttta cgcaattaaa tcaattttat atatgtttct cataatacca
     3421 atttgtgtta tcaaatatca ttaaataaga ttattctgct ttaaaataaa tattattaaa
     3481 aatactgtaa ttatagaaaa tccagaagta aatactacaa tttgtccagc taattcactg
     3541 tcagaatcca tctgttgtgc cattgtaaat gaagaaactg cagttggagc agataacatt
     3601 atcataagtg aagttaattc tacatttcta aatccaagaa aaatgctaat tggtaaaaat
     3661 atagaaggta caagtataag ttttccacat acgcccaata taatggtttt tatatgcccc
     3721 tttatagcag aaaaactaaa agatccgcct agaattataa gtgataaagg agtagctatt
     3781 ttagaaatat caccaatagt tttgtgtaaa aatgtaggta tattaatatt taaaattagc
     3841 attgtcaatc ctataaaaga ggcgattatg agtggatttg ttaagacgct tttaatcata
     3901 tgtcttatat ttactttgcc acctctaaaa atttcaagaa ttacaacaga taatacatta
     3961 aacataggta caataactgc aattaataaa gatgtgactc caatactctt atcgccaaat
     4021 agtgaaattg tcacaggaat accaaatata acgaaattgc tacgaaatac accttgtatt
     4081 aatgcacccc ttgtcttatt atctttttca attttaggaa taataaagac taagattaag
     4141 aagattgaaa aaataattat aacagctaca gccataagct taaaattaag agaaccatct
     4201 aaatctgtat tgtatatatt aaaaaataat aataatggca agaaagactt gaagcagaca
     4261 ttattcattt tatttaaagt atgagtatca aatagaccta atttttttag aacatatcca
     4321 agtgacattg tgagaaacaa aggaagaaca acatttattg ataggattaa attttccaag
     4381 gtttactctc cgtgattcta tgatttgata attcaattaa atttccatta gtttttttca
     4441 aataaataga tttcatatta ccaaagacac ctgtacattc tacaattcct cgcttaattt
     4501 gaatctcatt ttgttctaag tatttttttg gctcatcaat atttgtgttt attttaaaac
     4561 ataagttatc tctacttgct tttaaatttt taacataagg taaaaattta taacctttta
     4621 tgttaagatt tatcttaaca tttctagcct taagtttata cttttcgctc atttctttag
     4681 atgaaaaacc aaatttttta taaaaattga tataattttc tatatta
//
LOCUS       DAELSY010000220         4435 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.222, whole genome shotgun sequence.
ACCESSION   DAELSY010000220 DAELSY010000000
VERSION     DAELSY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.222"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1407
                     /locus_tag="KPG09_003602"
     CDS             <1..1407
                     /locus_tag="KPG09_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0968159.1"
                     /translation="IIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFIAEN
                     IMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKSNSS
                     VLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLLYKY
                     KKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNNNYE
                     ELTSKESVYYVSDSYKKMFFKDFEIEKINLYIKDTNEYDINLFKVQKSILEPEINSQA
                     TFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKFKNIVSENMKFTYPFNF
                     TSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLIKLG
                     ANNKDIEKIISKQLKIIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIYFIG
                     YFIYYFLTLKYALNMIKE"
     gene            1632..3245
                     /gene="ggt"
                     /locus_tag="KPG09_003603"
     CDS             1632..3245
                     /gene="ggt"
                     /locus_tag="KPG09_003603"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBG0968160.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(3378..4229)
                     /locus_tag="KPG09_003604"
     CDS             complement(3378..4229)
                     /locus_tag="KPG09_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBG0968161.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
BASE COUNT         1735 a          517 c          677 g         1506 t
ORIGIN      
        1 attatagagt tatttaataa atatccagaa aagtataagt ttaaaaaatt tataaagtta
       61 aaatgtgtac ttggtgtttt attaatagct atttcttatt taatgtcaat tagttatttt
      121 attgctgaaa atattatgct ttcagttttt atattagtta caataatacc tggaactttt
      181 ttattttttt catcaactat gtatatgatt attgatgctg ttaagaagag aaaaagtttt
      241 tattataagg gacagaattt aattgcattt tctgaacttg gatttaaatt aaagagcaat
      301 agtagtgtat tggctgtaat agcaatatta atagctacga gtgtaactat gctagggttt
      361 accatatcac tttattacga tatagatagg aacataagtg aaaattataa atacagttat
      421 accataaatt taggaaattc ttatgtaaat aacgaaattg acaaattatt atataaatat
      481 aaaaaagata ataagataat ctttgataaa acaatagaac ttatagacaa agatataaat
      541 attgaaatgt attataaaaa tggaaatagt tatatagaag atagaacttc tgtagatatt
      601 attagagaaa gtgattttaa aaaattaaga caatatcaga ataacaatta tgaagaatta
      661 acttcaaagg aatctgtgta ctatgtttct gattcataca aaaaaatgtt ttttaaagac
      721 tttgagattg aaaaaataaa cttgtatatt aaagatacaa atgagtatga tataaatctg
      781 tttaaggtac aaaaaagcat cttggagcca gaaataaatt cacaagctac gtttgattta
      841 attgtagtaa aagatgacgt atttaatcag ttaaaattac atggaaattc tagattaata
      901 agattaattg atataaaaaa tgaaaaaaaa gaactggatt tgactttaaa atttaaaaat
      961 atagtaagtg aaaatatgaa atttacttat ccttttaatt ttacatcaag tattgaaagt
     1021 tataataact tgataaaatt aagtggtcta atgctgttta taggcatatt tttgtctgta
     1081 gtatttcttt tatgtacagg tagtataatc ttatttaaac aattatcaaa tatatatgat
     1141 gataaggaaa gatatattat gttaataaaa cttggagcaa ataataaaga tattgagaaa
     1201 ataatatcaa aacagttaaa aattatattt ttaatgccat tagttgtagg tacagttcat
     1261 aatttatttg ctatgtctat agcacaaaag tttataccaa ggtcattact tgtaccaatt
     1321 atcataactc tagtcattta ttttatagga tattttatat attatttttt aactttaaaa
     1381 tatgctctaa acatgataaa agaataaatt atttttaagt caaaagaatt agatatatgt
     1441 tttgaataaa ataacttatt taagtgcaga tagaaaatac tagaattgag tttaagatat
     1501 ttatttaaag ataaaattaa agttaggaaa aagtctatat tttatatcat tttgtatcag
     1561 ttacattact tgatgttata taataaatct aagcagaata tcaaataaaa aatgacgagg
     1621 agctgaaatt tatgaagttt aatgcctacc aacataaata ctcatcaata agaaatgtcg
     1681 tgtatgctaa aaatggagca gttgcaacat caacaccatt agcatctcaa gctggacttg
     1741 aaatactaaa aaagggtgga aatgctgttg atgcagcagt tgcaacagcc gccacattag
     1801 cagtagtaga acctacaagc aatgggattg gaggagatgc atatgccctt gtttggatag
     1861 aggaagaaaa acgattgtat ggtttaaatt ctagtggctt tgctccagaa aatatggaat
     1921 taaaaaatta taataatatg aaagaaatgc caaaatatgg gtttggagca gtaactgtac
     1981 ctggaatacc cgcagcttgg tcagaattaa ataaaaaata tggtaaactt agcttaatgg
     2041 aatgcttatc tccagcgata aactatgcaa gagagggata cgtagtatct cctaatgtag
     2101 ctaaggtatg gaaaaaatca tatgaactct atgaaaaaga acttattgga gaagagttca
     2161 aaccatggtt tgatacattt tctaaagatg gtaaagctcc agaggctgga gatatattta
     2221 tatgtgaaga acaggcaagt acattagaag agatagcaaa tactcaggct gaaagctttt
     2281 atagaggaag acttgctgat aaaatagatg aatattctaa aaaatttaat ggggctataa
     2341 gaaagagtga cttagagtgt ttttatccaa catgggtaga acctataagt acagaatata
     2401 agggatataa aatatttgaa atcccaccaa atggtcatgg aataacagtt ttaatggcac
     2461 ttaacatatt aaaagaattg gaattagaag gaaatataga aaatgtagaa gacatacata
     2521 aaattataga gagtttaaaa ttagcttttg cagattctaa aacatatgta actgatatag
     2581 agcatatgaa ggtaaagatt caggaattac taagccaaga gtatgccaaa aaaagaagtt
     2641 tattaattga caataaagag gctttgtatc caactgcagg agagccatat tgtggtggta
     2701 cagtatacct ttgtactgca gataaggatg gaaatatggt ttcatatata cagagcaatt
     2761 acataaattt tggttcagga attgtgatac caagaactgg gattgcttta catagtagag
     2821 gaaataattt taatttagac cctaaacacc acaatgtagt aaaaccattt aaaaaacctt
     2881 atcatactat aatacctgga tttttaggta aagaagataa agcaataggt ccatttggtg
     2941 taatgggagc ttttatgcaa ccacaaggac atattcaagt gttaacaaat atgattgatt
     3001 ttggattaaa tccacaagag gcgctagatg ctccaagatg gcagtggata aaaggaaagg
     3061 aaatagaagt tgaaccagag atgccaaagc atataataga ttcattaatt gaaaagggtc
     3121 atgaaataaa agtaattcat gatactgtcg acatgggacg aggtcaaata atatttaaaa
     3181 cagaacagga aagttatatt tgtgggacgg aatcaagatg tgatggtcat attgcagttt
     3241 attaatatta tcaactaaaa tatatataat aaatttaaag agtatatgaa ataattagta
     3301 aaggcgaaag tctacaatga ctgacgcctt tacaaaattt aagctatcat atgctgattt
     3361 tatatagatt atgagcctta ttcttgacca gttttgattt cattgtcatc ataactaatc
     3421 cagtcactaa agctaccaat ataaatcttg tgaggtatat ccagttctct aagagcaaga
     3481 ctgttaacac aagcagatat ccctgaacca caagataaaa ttacttcatc ataattattt
     3541 aaatctttaa agaatttttt taaaaattct atatctttaa gtgagccatt ttcaaagtca
     3601 ctttttatta aatccttaca aaaataattt tttgcacttg gtatatgacc agcttttgag
     3661 taagcaggtt caactaaacc ctggtatctc tcattagcac ggcaatctat tattacagta
     3721 tcttttttgt ataatttaga cttaacatat tccattggaa caactaaatc attgtttata
     3781 tttggtctaa tttccttacc tgtacaactt ggtatattca ctttctcttc caattctcca
     3841 ccttccttta cgaaagatgt tattccccca tctagtacat agacattttt aagccccaaa
     3901 tgttttaatt ggaaaaataa tctgcaagct ccatttaaat ctccatcatc ataagttaca
     3961 atcgtagtgt cattatcaac acccattttt tctaattttt cttttagtat taaagggtct
     4021 tgtaaaggat ttttaccacc atgttctttt gtaggagatg ataaatcctt gtctatatct
     4081 aagataaaac tgcctttgat atgtcctttt ttataagaat caataccata agttctattg
     4141 ataagatcaa atctgcaatc tattacaact agattatcat ttttttctag ttttgatata
     4201 agttcttttg ctgaaataat gtttttcaaa gaatgtacct cctatgttta tattatcttt
     4261 attttaattt atattaatta ttaactttat aaactaatat tattataatt tattacataa
     4321 gtaaataaat ctttagaaat tgtaaaaatt ttatataaaa attcaaataa ttaatgaata
     4381 ttgaaaattt tagatatata ataaaataag agtatatttt atataatatg aaagc
//
LOCUS       DAELSY010000221         4413 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.223, whole genome shotgun sequence.
ACCESSION   DAELSY010000221 DAELSY010000000
VERSION     DAELSY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.223"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1072
                     /locus_tag="KPG09_003605"
     CDS             <1..1072
                     /locus_tag="KPG09_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968162.1"
                     /translation="VNNSAYVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLIVIVV
                     YSILFIGLNYISIDMSTYRDEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNIKDRI
                     VLLNTSNEELTNINMILDEIFDIKYIKVNSENIKFNKKKDLIEVNLKESLEPNDEVTL
                     DIEYEGYINVLTSWNQNKYIATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSKNNVY
                     SNLPLLNKTSNFLKKDYIFEGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMDIEEK
                     IKFLIETRNEILGDSKASVDAKKYVDEVDNVMESIKRKKINMILVADLPSNEFLYEDS
                     KGEKISITIYHWLSGNILIIDK"
     gene            1087..1959
                     /locus_tag="KPG09_003606"
     CDS             1087..1959
                     /locus_tag="KPG09_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0968163.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLTDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYIPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINNKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            1960..2748
                     /locus_tag="KPG09_003607"
     CDS             1960..2748
                     /locus_tag="KPG09_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968164.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYLGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVFYIARKSTVLKKSK"
     gene            complement(2844..3677)
                     /locus_tag="KPG09_003608"
     CDS             complement(2844..3677)
                     /locus_tag="KPG09_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBG0968165.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPLGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDKRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(3745..4374)
                     /locus_tag="KPG09_003609"
     CDS             complement(3745..4374)
                     /locus_tag="KPG09_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBG0968166.1"
                     /translation="MDENITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMI
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
BASE COUNT         1727 a          475 c          640 g         1571 t
ORIGIN      
        1 agttaataat tcagcatatg ttatagataa gattatacct acactatttg caattacttt
       61 aataatagta gcatatctga tattaaatga tattagaaag aaacagatta aatcattaat
      121 agttatagta gtgtacagta ttttatttat tggattaaat tatatatcaa tagatatgtc
      181 aacatataga gatgaggaac gtagttatgg agagtattta aagaaatcta atattagtga
      241 tattaaaata aaatcgcatg atatgaagat tgactttaaa agcaagtcaa atataaaaga
      301 tagaattgtt ttattaaaca catcaaatga agagttaaca aatattaata tgattttaga
      361 tgaaatattt gatataaaat atataaaagt aaattcggaa aatataaaat ttaacaaaaa
      421 aaaggattta attgaagtta atttaaagga aagtttagag cctaatgacg aggtgacatt
      481 agatatagag tatgaaggct atattaatgt gttgacatct tggaatcaaa ataaatatat
      541 agctactaaa gatgatatta tgcttccacc aggttcttta gcgtggtacc caaaagtaaa
      601 tcaatcaagt tgtatggctt ataaaataaa tttaagttct aaaaataatg tctattcaaa
      661 tttaccttta ctgaataaga ctagtaattt tttaaaaaaa gattatatat ttgaaggaaa
      721 gtcaaatgat attgcaatat atgcaggtga gtttaaagaa gaggtaaaag atgatataaa
      781 aataatctat ccatcagata gtaatgtaaa tatggatata gaggaaaaaa ttaaattttt
      841 aattgaaaca agaaatgaaa tattaggtga tagcaaagca tctgtagatg ctaaaaaata
      901 tgtagatgaa gtagataatg ttatggaatc aataaaaaga aaaaaaatta atatgatatt
      961 agttgctgac ttacctagca atgaattttt atatgaagac tcaaagggag agaaaattag
     1021 cataactatt tatcattggc tttcaggaaa tatccttata atagataaat aataaaggag
     1081 gttaatatga taaacaagtt agaaataaga aatctaaatt ttaattacaa aaataaaaaa
     1141 gctctggata atataaattt aaccctaaca gatggagtag ttgcattatt aggtcctaat
     1201 ggtgctggaa aatcaacatt aatgaggtta ttagtgactc tttatgaaac ttcaattgga
     1261 gagatagaat taaataatat aaagtatagc aaaaacaatg agaaaatcaa ggcaaatgta
     1321 ggatatatac ctcaagattt tgatatgtat aataatataa atgggcaaga atacttagag
     1381 tttgtagcta aaatgagagg tgtatctaag aatgatttaa agaaacatat acaaaaagta
     1441 gtatcaaaag ttaatctgga caagtttata aataataaaa taggtacata ctcaggtggt
     1501 gtaaagagac gactaggtat agcacaggct ttaataggtg attctaagtt aatagttatg
     1561 gatgaaccta cagttggtct tgatccagag caaaggaatg aatttagaag attgttacca
     1621 gaaataagta aaaattctat agtattaatt tcaactcaca tagtagaaga catacaattt
     1681 aactgtgaca aattaattat attaaatcaa ggcaaaatat tatatgatgg tacaattaat
     1741 aaatttattg atatggttga ggtgtattca gtagtagttt ctaatgatga atttgaatat
     1801 ttagaacgta atattgatat tattgattat agaagagtta aagatggtgt gcaagtaaag
     1861 tatataggta aaaatacaga gaaacttaag gatagcttaa ggatagatac aactttacaa
     1921 gatgcttata taaacttttt acataaggag ttaaaataaa tgtgtatatt ggagttaata
     1981 aaatttgagc ttaaaaaaat agagcttaaa catgtgttaa tatttgtaat tatttctata
     2041 ttatgcatat ttattacagg catattagat tataatgagt taatatttta taatgatgta
     2101 cctataatgg atacatattc tatagcagga tggttaggaa ttataaagat atcagatgtt
     2161 tttataccaa taatgtcagc aatcacaata atttatgtgt tttcaaaaga ttatttgggt
     2221 aatataaatg agatacttac tttatataat aaaaaaaaat ataatcaatt tatagtattt
     2281 agatggtgtg ttatattagc tatatatact gtattattat taatttcaat tacaatagta
     2341 tatacattta cgtcagtgaa aggttttaac atagacaaaa tagattttgt ttatagtgta
     2401 aagccaatag atgtgttttt aaagacattt cctacattat tatggtatac aacatttcca
     2461 ctaatgatat taacattaac aaaaaataga tttacaacaa tagcaatatt aattacatat
     2521 acatttgctg atatattttt tattttacat atgtacccat ttgttagtat gattaatgta
     2581 aatggatttt atattcaaaa gatgttttat atgcctaata atatagatgt tagatttaat
     2641 gaattaaaga attatttctt tataaataga ggttctctag tagttatttc tataataata
     2701 gttttttata tagcaagaaa atcaacggtt ttaaaaaaga gtaaatagtt taatatattt
     2761 tttataatgt tatatttata tagaatagtg actttaaaaa atttaaagcc actatttttt
     2821 aatatgtttt atctatataa ctattatttt tctaggtttt ttaatatttc gtaatagtgt
     2881 gttgtggcta tttcacttga ctcgcctttt ttacaaaaag ataatttaga tagttttgga
     2941 actacacgtt caacgacaat tttattacct atcattttag attcaagtat cttcaatgct
     3001 tctaaaaatc tcttatcctt ttttgcacga ttataaaatg ataaaacgta gacataaagg
     3061 aataggttat aattacggaa aggatattca acttgcataa ataatgtgcc aattccatag
     3121 tgacaagggc cgagtggttt tttaatagtc caatggtcaa gtagaaaatc tacagctttg
     3181 tcaagttttg attctttatt tagataatta ctaaaacgga atgcatttaa tatattaaga
     3241 gttggtagtg gatttgagta ttcagtttct ggacctcgac caaaactaaa tttattgcaa
     3301 cgccacccac catcagtata ctgtgtatct aaaaaatgtt gaaacgtttt ttgcattcta
     3361 atgtcagatg catatcccat atgacagagt acgttagcag catttgcagt atgacaaggg
     3421 taaatacctc catttggata tagtttgaaa cgtccatctt ctcgccatgt gctaaatatc
     3481 aagtcagcac attctttaag taaagtatca tcagggttca cacctaactc taatagataa
     3541 agtacactat caagagtaga gaaaggagaa ccttttatta aacgcttatc aggtgtagtc
     3601 caatagtctg caccattatc atacctgtgt gataatatta tttcaatatc tgataaagat
     3661 tgattttcta cattcatttt ttaccccatt tcatattata ttctattaaa tctatcaaaa
     3721 actttattat aatattattt atatttataa ttgatttatt ttatttaaaa ttgcatttat
     3781 agctgtatca ggaacaactg ctaaatcttc tgcattgaag taataaacta tattagaatc
     3841 aaataatata tttgaatttg gaggaaattc atcatctcca aaccaaagta taaatatcat
     3901 agatgtatta cccatatatg taaacctata agctaaatct cccatgttta ctttttcagc
     3961 tccaattttc tccataacta acttatattt gtctatttga gatgtaaaaa cttctgaaag
     4021 cctatataga gtcctgttta aaaaatttgg ataataaaca ttgcctcctg gtatttcttt
     4081 aaaagttaca tatttattag ttctagggat acctttagca ttgattaaat atctaactat
     4141 caagatttta agctcaaagt tattgcaaag gctaccatta ctattccaaa cttctccact
     4201 tggatagtct ataatatatt gtctatcaaa aaaattgagt gtgaatctat ttaaatcatt
     4261 attgaaaggt atattactta aattactaat tgtatgaggg tctaattctt tgagtttttt
     4321 tcgaacatag tcaaatggaa cattataatt attttttgtt atattttcat ccataatttt
     4381 ttcctccaat ttaaacaatt aaaagtgtat tta
//
LOCUS       DAELSY010000222         4409 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.224, whole genome shotgun sequence.
ACCESSION   DAELSY010000222 DAELSY010000000
VERSION     DAELSY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.224"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            399..2309
                     /locus_tag="KPG09_003610"
     CDS             399..2309
                     /locus_tag="KPG09_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG0968167.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILSYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(2372..2995)
                     /gene="ytaF"
                     /locus_tag="KPG09_003611"
     CDS             complement(2372..2995)
                     /gene="ytaF"
                     /locus_tag="KPG09_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBG0968168.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLELNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            3180..3824
                     /locus_tag="KPG09_003612"
     CDS             3180..3824
                     /locus_tag="KPG09_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0968169.1"
                     /translation="MEQHSKVGNFGDLLKVLLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQRPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            4038..>4409
                     /locus_tag="KPG09_003613"
     CDS             4038..>4409
                     /locus_tag="KPG09_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG0968170.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTI
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCIFLENCDFNRTI"
BASE COUNT         1915 a          423 c          650 g         1421 t
ORIGIN      
        1 tgattacaaa gttagattgt attggaaact acacatacat gaattgatga caatataagt
       61 aattccaaaa tataaatgaa agtataagct tattttcact ataaaaattt tcactgtaca
      121 aaaacattaa taacattatt tttattgaca taaatatata atttatggaa taatattaga
      181 agcactggta taagtgttta aataagaata gaaaaggaga agtgttttat gaaaaaaaat
      241 actaacatta atgagtccgg agttaaactt cttaataaat aaggagtttg atatataata
      301 actccttatt tattaagttg ttcgcaaaat aactattatc aaagtaaatg atagggtgat
      361 ttaaaagacg atgacttata aataaaggag aaaaaataat gaatagtaat acatttgaaa
      421 gattgcaact aaatgaagtt aaagacttaa taaaaataca ttgtgtaagt tcgctaggaa
      481 aaaatttaat agataaatta actcctagtg ggaatattgg agtagtaaga aggaaattag
      541 cagaaaataa agaagctaga aagataattg aaaatagtaa tcacatacca cttgaaggat
      601 tgtttaatgc aggttctaca atagataaaa ttgaaaaagg aatgataata gaacctgttg
      661 aacttgtaaa tattgaagat tttttaagag gttgtaggaa gatgaaagct tttatgttag
      721 aaaaagaatt ttattcacct acattaagtt cttatgcttt aaatataaca gaatgtaaaa
      781 gtatagaaga tgagataaat tactgtataa aatctaataa agttgattct aatgctagta
      841 aagaattaaa aaagataaga cgaaatatag aaattacaga aggaaagata aaagatagat
      901 taaataaatt tataacatca actgtaaata aaaagtatat acaagagttt ataataagta
      961 aaagaaatga tagatatgta ataccaatta aatcttctta taaaaatgaa gttaatggta
     1021 ctatattaga tacatcatca aaaggaaata ctgtatttat tgaacctatt agtgtttcaa
     1081 atttaagtac agagttaact atgctaaaag ctgatgaaac tattgaagaa tacaaaatat
     1141 tatcttattt aacagaactt atatttgaga agataagtca gataaaatta aatatagaga
     1201 tactttcaga atacgatatg gtatttgcaa aagctaagta tagtcaaaaa ataaaaggaa
     1261 ttactccaaa aataaataat aatggctata taaaaataat taaaggtaaa catccacttc
     1321 taacaggaga tgcagtacct ttagattttg aaataggcaa aaattataga agtcttataa
     1381 taacaggtcc taatgcagga ggaaaaactg taacccttaa gactgttggt ttactaactt
     1441 taatggttca atgtggattt gatatttctg caaaagaagg tagtgaattt agtgtatttg
     1501 aaaaagtttt tgtagatata ggggataatc aaagtattga aaatgcctta agtacttttt
     1561 catcacatat aaaaaatatt gcagagatta tgagtttatc aaataattct actcttgttc
     1621 tgtttgatga aataggaagt ggaactgaac ctaatgaagg agctggttta gcaatatcat
     1681 tattagaaga gttttacaaa atgggatgta taactatagc atcaactcat tatggagaga
     1741 taaaaaagtt tgctacatta catccagaat ttgaaaatgc tggtatgatg tttgataaag
     1801 aaacgttaga accattgtat aagttaacta ttggaaaatc tgaagatagt aatgctcttt
     1861 ttatttctaa aaaaatggga ataaaaaata gagttttaga tagagctaaa gaatatgtaa
     1921 ttgatagaaa ctacaattta gatttagtta aaagaagtaa gctacaatct aatattgaag
     1981 ataaaaatag ttctgaagtc attttaagct atacagatta taatgtaggg gataaggtaa
     2041 aattactaga tgaagatgat tttggaattg tatataagcc tatggacaag tttaataatg
     2101 tagaagtatt gtttaatgat aattttattt cagtaaatat aaaaagatta caacttagca
     2161 taaaagcatc agaattgtat ccagaagggt atgatttaga tgtattattt actagtttta
     2221 aagaaagaaa attagaaaaa gatattcaaa gaggttcaaa aaaagctctt aaaaagattc
     2281 agaaagaaat tagagagaat agaaaataaa tataaaaagc acaaaagaaa gcaatctaaa
     2341 gagaaaagtt tatctttaga ttgctttctt attaacatat cttcataaat ccaatgacta
     2401 acaagattaa accacttaac caagataagt ttaaatttac gtttttaaca aacatatttc
     2461 ctagtaaaca accaagtaat actgcaatta tatttgatat taaagaaaat agaataactt
     2521 gtatataatt cacacctaca aggccactac caaaaccgat agctaatcca tctaaactta
     2581 aagcaagtgc aagtgtaaat gcttcagcag atttaagcac tttagattta cttgtatcta
     2641 agacatcagg ttcgatactt acattaaaat ttaattcaag gtctaacata ttaaaattat
     2701 aattctttga gtgtatagat tttttcttaa aataagattt aaaaaaactt tcaattagtc
     2761 tagcacttcc taataaaaat aaaataaaga aacatattaa cattgttata ttttgaggta
     2821 aaaaatctct aactattgta cctgcaaaaa gcgatattgc taaaatagct gaacaaacta
     2881 cgctgataat tatatttgaa tataagggaa ctttaacttt atttactcca tatccaaagc
     2941 ttgtcacaaa ggcatctata caaagagaag taactaataa aattgattca atcacagtag
     3001 attaacatcc tttctcttaa attttgtact tatattagtt atagtattgt aaaaatggat
     3061 aaattgttac agataatcca ataaattgtt gataaaattt caacatcata caacacctaa
     3121 ataacaaaag aatcatatca tataatataa aaccaatcat aataaagcag gtgaaaataa
     3181 tggaacaaca tagtaaggtt gggaatttcg gtgatttgtt gaaagtttta ttagatgaaa
     3241 aagaattatc aatgggagaa ctaagtaaaa aaagtggaat tgataaatct actatatctc
     3301 gtatagctaa taataaacaa aggccaaata taaatcattt agaaaagatg gcaatacatt
     3361 taaatattaa cctagaagaa ctgttgaaag cttcaggata tgaatttaga aacagcaata
     3421 atcaaatatt taatgcagat agtgatttta gtaactttga tgatattctt ggatttgcaa
     3481 atttaatcaa tgataagaac tttaatggaa atattgaaaa agagttaagt aagtgtaagt
     3541 tatatgtcca aacagatgaa ggtaaaaaat tactttttga taattttaat aaaaaaatag
     3601 attctattga aaaacaaggt caatttacag atagattaag acaaatgtat gctgattttt
     3661 gtacagatgg acttacaata aagaaatatt tattgattgg aagtatgcta ctttactttg
     3721 ttatatcaac agatgtgatt ccagacttcg tgttcccaat aggatttatg gatgatttag
     3781 tagcattaaa tatagttaca aaattactta aaaatgataa gtaagtttta gaataatatt
     3841 taatatataa ataaatcaaa gtatataaaa tttattattt tttatatgaa acaaattaaa
     3901 agcacaatga agtagattaa aaatttaaaa ctaagaatct aatatatatt ttgtaactat
     3961 ttaagtatat aaaataaaat aatatccaaa ctaatacaaa atagtaataa ttgttttgta
     4021 ctggaggata tatatttatg aattgtaaat ggatatctaa gatagatgag agaaaaaaat
     4081 gtcatagaga agcagattct tctggatatt gtatctttca taaagaaaat aagtctgatg
     4141 aagagataca gttgatgatg gatactattc ataaagaaga gataagtgaa tttaatggat
     4201 ttgtttttga aaatgaattt aatgcggaag aaatattaac ttacaattat aaaatactgg
     4261 atttttctga atccatattt aaacaaaaag caaattttaa aaaatatatt tttaaaaaga
     4321 atattatttt taactataca gaatttagag ataaagtttt atttaatggt tgtatattct
     4381 tagaaaactg tgattttaat agaaccata
//
LOCUS       DAELSY010000223         4341 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.225, whole genome shotgun sequence.
ACCESSION   DAELSY010000223 DAELSY010000000
VERSION     DAELSY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.225"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            172..1347
                     /locus_tag="KPG09_003614"
     CDS             172..1347
                     /locus_tag="KPG09_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0968171.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQAYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            1382..2920
                     /locus_tag="KPG09_003615"
     CDS             1382..2920
                     /locus_tag="KPG09_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG0968172.1"
                     /translation="MQGALKENKKKSGGGFLGWIERVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQVI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            2952..4154
                     /locus_tag="KPG09_003616"
     CDS             2952..4154
                     /locus_tag="KPG09_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0968173.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFIQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     SEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     IWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
BASE COUNT         1615 a          505 c          856 g         1365 t
ORIGIN      
        1 tagaaaaata ctataatata tcagcttaat acagaaaaaa gttaaaaata ttgagaaaaa
       61 taaaaatgaa aacgttttat attgacatag agaaaaagta gtgataccat ttaaatatag
      121 tttagtagct aaactatatt taaattataa taactatttt agggggatgt tatgaattta
      181 aaacaattaa gtgaaaaata cgaagaatat gtaattcaaa tgagaagaga atttcataag
      241 tatccagaat tatcttttaa agaaataaga acaacccaaa gaataaggga agaattacaa
      301 aaaatagggg tcccttttgt atctattgaa ccaaatatcg taatagctac tatacaaggc
      361 aaaaatgaag gtaagacaat tgcaattaga ggagatatag atgcgcttcc aatgcaagaa
      421 gattcagatg ttgaatacaa atcagaatat gatggaataa tgcattcttg tggacatgat
      481 gctcatggag ctatgttact aggacttgca catatgctaa atgatataag aaaagattta
      541 aaaggtagag tattattagt ttttcaagca gctgaagaag ttggtggcgg acacaaggag
      601 ataatacagt actttaagga aaatggagga ccagatagat taatagctac tcatgtttgg
      661 tccgatattg aagctggtaa aatttctgtt gaaccaggac ctcgtatggc tggtgggtct
      721 ggttggagaa tagacataac aggtagagga ggtcatggtt ctagaccaga tcaaagcata
      781 gatcctataa agccagcttg tgatatagtt ttaaaagtat cttctatacc agttaatcat
      841 ataagtgtac tagaacaatg tgttgtacat gcatgtgcaa tacatggagg aactacaata
      901 ggaaatgtta ttccaaacaa tgcagaggta ataggtggat atagatattt tactgaagaa
      961 tcaggcaaaa aagtgtttga cattataaag caaatgagta atggtgttgg acaagcttat
     1021 ggagtggatg tgaaggttac tcatactgga ggcataggac ctgttatcaa tgatgaagaa
     1081 gcagttaaga tggctaaaga aatagtttct aatatagatg gattggagtt agattcttat
     1141 gagccaatat gtgcatcaga ttgttatggt gaaatattaa aagagtattc tggtttttat
     1201 tgttatctag gggtaggtaa caaagaaaaa ggaataatat atgcacaaca ccatcctaag
     1261 tacaatatag atgaagatac attaaagtta ggttgcgaat ttatggctaa atatgcagtt
     1321 gaatttttaa ataaaaataa taactaaaat aaatcgaatt ttataaatgg gggggatgaa
     1381 aatgcaaggt gcattaaagg aaaataagaa aaaaagtggt ggaggatttt taggatggat
     1441 tgaaagagtt gggaataaaa tgccacatcc tctagcatta tttacatata ttacattgat
     1501 agtagtagca ttatctgcaa tagcaagctt tttaggtttt tcagcaacaa gtcctgctac
     1561 aggaaagtta ataaaagtta tgaacttaat aagtgcaaaa ggtttagtac tatttatgca
     1621 agagtttgtt aagaacttcc aaaacttccc tgtgcttggg gtagttttag ttatggcagt
     1681 tgccacagga gtttgtgaaa agacaggatt tttttcaact gccataaaaa tgagtttatc
     1741 aaaagttaag gggaatgcgg ttgtatttat aatagctttt ataggagtat ttgctaatca
     1801 agctggtgat gctgcttttg tattagtacc aactcttgca ggagcaatat tttatggatt
     1861 aaatagaaat ccattagctg gtatattctg tggatttgca tcagttggtg gtggatttgc
     1921 aacagctgta attcctgggg gatgggacgt tacgctaacg ccaataactg tatcagctgc
     1981 tcaaactatt caacctgcat ttgatatgac attattagct agttattatg cactatttgt
     2041 atctgcgttt atagtagcta cagtatcaac aatagtaacg gttaaattta tagaacctaa
     2101 attaggtaag tatactggta aaccagaagg tatagatgat aatcaaggtt ttgaggtcac
     2161 aaaagaacaa gcaaaagcag caaagttagc aggttttaca gttttagctt atgtagcgtt
     2221 attaattgct ttgtgtatac cagctaatag ttttttaaga agtccagaag gttctttaat
     2281 atttggagca ccacttatgt cttgcttaca gttttttata gtaatactat tcttcttgcc
     2341 agggattgtg tatggaatga gggttggtaa aatcaagact gtaaaagact taaatgatat
     2401 attatgtcaa tcaatggcag caatggcacc gtttatcgta cttgcaatag ttataggtca
     2461 attcttaacg ctattctcag tgtctaattt agcacaggta ctagctataa aaggtggagc
     2521 attactcaac tcattgccac taccaccaca agttataata ctgctattct tagtactagt
     2581 agcgtttata aatatatttg ttataagcgg aagtacaaaa tggatgatat ttggaccagt
     2641 atttgttccg atgttaatgc aattaaacat acatccagca tttactcagt ttgtatatag
     2701 attaggagat tctgtaacta atcatttatc accattaaat gcattcttta taatactttt
     2761 agcaacagtc caaaaatacg ataaaaatgc aggtatggga actatattct cagctatgat
     2821 accttacact ataggttact ttgtagtact tgcagtttta gtagtagttt ggatgacaat
     2881 aggtatacca gtaggaatag gtggacaagt ttggttataa aacatattta ataattttgg
     2941 gaggaaagaa aatggaaaca actgaaataa gatacaattt tgatgaagta atagatagaa
     3001 gtggaacaaa ctgtcttaaa tatgatctta agagtcaatt tatgccagaa tctccagaag
     3061 atgcactacc actatgggta gcagacatgg attttgcatg tgctcctgag ataattaatg
     3121 caatgcatga aagaatagac agaaaaatat ttggatatag ctctcaacaa actgatgatt
     3181 attatgaagc tgtatgtgga tggtttaaga gaagatttga ttggaaaata gaaaaagaga
     3241 atatattctt tagcccaggg gttgtgcctg cgttaggatt tttcatacaa atattaacac
     3301 aaaagggtga tggtatcata atagaaaagc ctgtatacta tccttttgaa gataaaataa
     3361 ctaataatga aagaaaagta gttaatagtc ctttaaagtt tgaagatggt cattattcta
     3421 tggattatga tggatttgaa gaattagcta aagatgaaaa taataaggtg tttatattat
     3481 gtagtcctca taatccagtt ggtagagtat ggaaaaaaga agaacttaga aaaatagtag
     3541 acatatgtaa gaaatatgat ttgtggataa tatctgatga aatacattgt gatattattc
     3601 gtaaaagtga aaagcatata ccattagaag tagcatgtcc tgattataaa gatagaataa
     3661 taacttgtac agctccaagt aagagtttta acttagctgg tatgcaatta tcaaatatag
     3721 taatcaataa taaggaatta caagaaaaat ggatgcttga aacagattct aaattagcta
     3781 tattcctacc taatccattc gcaatagttg ctactaaagt tgcttataat gattgtgaag
     3841 attggatgaa tcaagtaaat gagtatatag atggtaatat taaatatgta gaagattttg
     3901 ttaatgagta tatgcctaaa gtaaagataa taaaatctca aggaacttac ttaatttggt
     3961 tagactttag agggtatgga ttagatgcaa aacagcttga atatgtaatg cttaacaagg
     4021 cgaaggtatt gctagatgaa ggatacatat tcggagaaga aggagcagga tttgaaagaa
     4081 taaatgttgc ttcgcctaga acaattatag aagagtgtat gaatagaata aaatgtgcat
     4141 ttgaaggatt ataatagtaa tatctaaata atatatttgt gtattttaaa aaaagactat
     4201 gttaaaaaaa tatttcaaag catagtcttt taaagtttta tgatgagttt aaaaaatttc
     4261 aaaacgttac tattattgat aatcttcact tatatgttta tattagaaat aattccacaa
     4321 tatgctcatt ttctaaagtt g
//
LOCUS       DAELSY010000224         4292 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.226, whole genome shotgun sequence.
ACCESSION   DAELSY010000224 DAELSY010000000
VERSION     DAELSY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.226"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            39..1325
                     /locus_tag="KPG09_003617"
     CDS             39..1325
                     /locus_tag="KPG09_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG0968174.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            1411..2718
                     /gene="grdB"
                     /locus_tag="KPG09_003618"
     CDS             1411..2718
                     /gene="grdB"
                     /locus_tag="KPG09_003618"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2458..2460,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG0968175.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVVTGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            complement(2934..3158)
                     /locus_tag="KPG09_003619"
     CDS             complement(2934..3158)
                     /locus_tag="KPG09_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0968176.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(3255..>4292)
                     /locus_tag="KPG09_003620"
     CDS             complement(3255..>4292)
                     /locus_tag="KPG09_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBG0968177.1"
                     /translation="ITFFFTTIGLEISINLIKKGGSVLFRYWILCGVLAFCQNILAIT
                     ISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVGVGLSAATIGLIMGG
                     ILGCPVAKFLINKYKLKPSSNIGLSIPRYNRNLKRFGSKFFRNKNRIKNNNFSETITP
                     SVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVIFRNLNDKINILELN
                     FKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVIFIILFSIFICFRVL
                     GKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLVVPLVSGFLLDAISI
                     PCILFFINILT"
BASE COUNT         1647 a          532 c          784 g         1329 t
ORIGIN      
        1 ttttaattgc gattaaatat attaaggggg attttcatat gaagctagag ctagggaata
       61 tatttataaa aaatgttgaa tttggagaaa agacagaagt aaaagatgga gtactatata
      121 ttagtagcga agaaatagaa aagatagtat tagaagatga aaggattgta agtgtaaatg
      181 ttgaacttgc aagacctggc gaatcaataa gaatagctcc agttaaagat gtaatagagc
      241 ctagagttaa aattggagat gaaagcaaaa tatttcctgg aattataaat aaagtgaaga
      301 ctgtaggaag tggaagaacc catgtacttt taggagcttg tgttgtaact tgtggaaata
      361 tagttggatt tcaagaaggc gtaattgata tgagtggtcc tactgctaaa tatacacctt
      421 tttcaaaaac aaataatata tgtattgtta taaaagcaaa ggatggtata gatactcatg
      481 attatgaaga agcagctaga attgcaggat taaaaatagg tgcatatgta ggtgaggcag
      541 gaagagaagt tgagccagat gaagtcattg tatatgagac aaagccatta ttaaaacaag
      601 taaaagaata tccagacctg ccaacagtag catatgttca catgttacaa tcacaaggac
      661 ttttacatga cacttattac tatggggtgg atgctaaaca gatagtgcct acatttatgt
      721 atccaactga aataatggat ggagctataa tatctggaaa ctgtgtagca ccatgtgata
      781 aggttacaac attccatcat ttaaataatc cagttataca tgacttatat aaaagacatg
      841 gaaaagattt aaactttata ggagttattt tgactaatga gaacgtattc ttagtcgata
      901 aagagagaag ctctgatatg gtagctaaat taatagaatt tttaggagtt gatggagttc
      961 tagtgacaga agaaggttat gggaatccag atactgacct aatgatgaat tgtagaaaat
     1021 gtagtgaagt tggagcaaat gtagttttga taacggatga atttcctgga aaagatggca
     1081 agagtcaatc tatagcagac gctactaagg aagcagatgc agttgtttct tgtggtcaag
     1141 gaaatctagt ggttcatttc ccagcaatgg aaaaaattat aggaacttta gattatgttg
     1201 aaatgatgat aggtgggtat aaaggatgct taaatgaaga cggaagcatg gatgcagagc
     1261 tacaaattat aatagcatca actatagcaa atggctataa tcatttgaca gcaagatatt
     1321 attaaataag tatttgaata taaagttctg ttgatgtaat cgttggtcta attgatgtaa
     1381 taactataaa gtgatttgga ggattaagag atgagtaaaa tcagagtggt tcattatata
     1441 aacaatttct ttgctggtat aggtggagag gaaaaagcag atattccgcc agaaaaaagg
     1501 gctggtgtag taggacctgg aatagcattt cagagtcaat ttaatgatga tgcagagata
     1561 atctcaactg taatttgtgg agatacgtat tttggtgaaa atattgagag tgcaactcaa
     1621 gaacttttaa aaatgataaa agaagaaagc cccgatttat ttattgcagg cccagcattt
     1681 aatgcaggta gatatggagt agcttgtggg actatttgta aggcagttga agaagagtta
     1741 aatattcctg tagttacagg aatgtataaa gaaaatccag gtgtagatat gtttaaatta
     1801 gatttgcata tagtgtctac aggaaattca gcagcaagtc ttagaaaggt agttccaata
     1861 atgacgaaac taggtcttaa attggttaga ggtgaggaaa taggacctcc tgaagaggaa
     1921 ggatatataa tgagaggaat aagaaaaaat ttcttccatg aacttagagg gtctgaaaga
     1981 gcaattgata tgttggttaa aaagatgaaa ggggaaagct ttgaaactga gtatccaatg
     2041 cctgaatttg atagagttac acctatgaaa cctgttaaag atttatctaa gataaaactt
     2101 gccctagtaa cttctggagg gatagttcca attgataatc cagacaaaat tgaatcttca
     2161 agtgctacta aatatggaat ttacgattta acagatatgg attctatgtc aaataaagaa
     2221 tttacaacta tacatggggg gtatgacaga gcatatgtcc ttgaaaatcc aaatttagtt
     2281 gtaccattag atgtagttag agagcttgaa aaagaaggtg taattggaga attagtagat
     2341 tattttataa ctactacagg aacaggaacg agtgttggca attctaagag atttggagaa
     2401 gagttttcta agaaattgtt agaagatgat gtagatgctg taattttaac ttctacatga
     2461 ggtacttgta ctcgttgtgg agcaacgatg gtaaaagaga ttgagagagc tggtatagca
     2521 gttgttcata tatgtacagt agtaccaata tcacttacaa taggagctaa tagaattgtt
     2581 ccagcagtag ggataccata tccacttgga aatcctatgt taggagaaga ggaaagtaaa
     2641 aaaattagaa agaatatagt tttaaaagct ttaaatgcac taacagagga tatagaagaa
     2701 caaacagtat ttgaataata aaaatataat atagaaataa aaaaagatat cataaaatag
     2761 tttttgaaaa ttattttatg atatcttttt gctatggatg gaatgaagaa agcaattaag
     2821 taataaaaat atttatagta aaacagccaa ttaaagtata aagaatgaaa aaaataaata
     2881 gaagattata cataaacaaa aaaagagatt ttaaaatctc tttttattat atattaatta
     2941 ttttttaaat ttatattttt tgcatcagat tttctaatag ctaagtcaaa ccctctaaat
     3001 ctaatcacta tagggtcatt aagaggagct atctttttca cttcaacttc agtgccatct
     3061 attagtccta aggctagaag tctcttcata agtttttcat ttccagctat attgtctata
     3121 attccctttt gtccaagttt taagttgtaa acagtcatta ttaacacctc ctaatgaaaa
     3181 tcattatcaa actttatatt tataatatat aacaaaaaga gtaaattgtc aatatataac
     3241 aaaaaataaa catactaagt tagtatgttt ataaaaaata gtatacatgg gatactaata
     3301 gcatctagca aaaaaccact tactaatggt acaaccaaaa aggcttttgg agaatcacca
     3361 tatttttgtg tcaaagaact catatttgcc aaagcgttcg gagtagctcc tattccatgt
     3421 ccaatcaaac cacttattat gatggcagaa tcaaaatctt ttccaagtac tctaaagcaa
     3481 ataaatattg aaaataggat tataaagatt acttgaaaag ttactattaa gagaatagga
     3541 ggtaataatg tagataattc aaataaatct atattcataa gtgataaagt taaaaaaatt
     3601 cccaatgaaa gttcttttaa aaaatctata agtttaaaat ttaattctaa tatatttatc
     3661 ttatcattta aatttctaaa aataacagct gaaaacatac aagttacaac agagggtaat
     3721 agtatattaa acgttatata gaagcatcta cttataattt ctcctgtatt tatacaaata
     3781 aatatgagaa gaacctgttc caaaaataca gatggtgtta ttgtttcgga gaagttatta
     3841 ttttttatcc tattcttatt tctaaaaaat ttacttccaa atctctttag atttctatta
     3901 tatctaggaa tcgacaagcc tatatttgat gatggtttta atttatattt atttattaga
     3961 aatttagcta caggacatcc caatatacca cccataataa gtcctattgt tgcagcagat
     4021 aatccaaccc caactgctcc ttctataccg agattttcta ttgtaagtcc aaatgcagca
     4081 gaataaccat gaccaccttc catagataca tttccacaca taagccctaa aagaggttca
     4141 agttttataa tctttgatat tgtaatagct aatatatttt gacaaaatgc caatactcca
     4201 cacaaaatcc aatatctaaa taatacacta ccacctttct ttataagatt tatgcttatt
     4261 tcaagtccta tagttgtaaa aaagaaggtt at
//
LOCUS       DAELSY010000225         4277 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.227, whole genome shotgun sequence.
ACCESSION   DAELSY010000225 DAELSY010000000
VERSION     DAELSY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.227"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..141
                     /locus_tag="KPG09_003621"
     CDS             <1..141
                     /locus_tag="KPG09_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968178.1"
                     /translation="RDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIKMDNLIMDSL
                     GI"
     gene            373..1584
                     /locus_tag="KPG09_003622"
     CDS             373..1584
                     /locus_tag="KPG09_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG0968179.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            1776..2036
                     /locus_tag="KPG09_003623"
     CDS             1776..2036
                     /locus_tag="KPG09_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBG0968180.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            2389..4101
                     /gene="ptsP"
                     /locus_tag="KPG09_003624"
     CDS             2389..4101
                     /gene="ptsP"
                     /locus_tag="KPG09_003624"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBG0968181.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASIHGRLRIMFPMI
                     SSLEEFLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
BASE COUNT         1761 a          402 c          769 g         1345 t
ORIGIN      
        1 agggataata attatgaaga gatagagaat ttatcaaagc aaataatatc aattttatta
       61 aataagtcta tagataaagg aaaagtagaa aaattgcaga taaaaatgga taacttgata
      121 atggattctt taggtatata agcagttgta agtattaaaa aattataatc ctttaattgt
      181 ttatttaaat ttagaaataa aaagttagcc atataaaaaa tattaaaaat atatactttt
      241 caatattttt ttgataaaat aagaatgaat ttgtgcaatt ttatttttct ccaataacta
      301 aaaatagttt tttgaattaa tatttgctta agaataaaaa tcagaaacgt ttttgatata
      361 gtaggtgata atatgaaagg attagtttta gaaggtggag gaacaaaagg agcctaccaa
      421 ataggtgcat ataaagcttt aagagagctt ggtatagagt ttcaaggagt aactggcaca
      481 tcaattggtg ctttaaatgg agcatatata attcaaaatg atattgaagt aatggaagat
      541 atttggttaa attatgacta tactcatttt atgaatattg atagagaaac ctatgagagg
      601 tttaaaaatg tagatttcac tactaaaaat ctcaatgcag tgtttgagct tataaataaa
      661 gctagaaaaa atgaaggtat agatataagc ccattaagac aactaatgga gacaacatta
      721 aatgaagaca taataaggaa atcaaaaaaa gactttggac ttgttacggt ctggtgggat
      781 aaaaaaatta atccatatcc attgtatatc gaagatatac ctaaaggaag attgattgat
      841 tatttaattg ctagttccag tttgcctatt tttcaaacga acatgatgga tgataagttg
      901 tatcttgatg gaatgttttg tgataacatg ccaataggac tcttggaaga caaaggttat
      961 gaagatgttg ttgtaattag gttgattgat gattttattg ggaaaatgaa tctaaatcga
     1021 catcaaaata taaatataaa aactataata ccatcagaat atctaggagg ttctcttaat
     1081 aaagatagag ataatgtaga aaaaaacatc cgactaggtt acttagatac tatgaaagca
     1141 tatgaaagat gcactggaat taagtattat tttgatgtag agtataaata taatgaagaa
     1201 tattgttttg ataaatttag aagtatgagc aatgaaacaa tacaatatat ctgtaattta
     1261 ttgaatataa aaagagatgc ttcacttaga actgttatgg aaagtgtaat accaaagctt
     1321 ggtgaggtgc ttggattgcc aaaggacttt tcatataaag atttgtttta ctgtatatat
     1381 gaaaagaaac ttgaagaaaa taatataaac aggataaaat tatatgattt taacaaggtt
     1441 atagatgttg taaatacaaa tattgattct aatagaaatt tagcttctaa ttttgaagtt
     1501 agacctaaaa ttagtattaa aaagaacaaa aacacaaaat tattgacaaa ttgtattata
     1561 aaagacataa aaaatcaaag ttaaataaaa aacaaaaaaa aatttgtaat tatactcgaa
     1621 aaaagtgtat attagtagta taatattttt gtagcactag tgggaacaaa aaatgaccat
     1681 aggacaaaaa agtaccactt agtaccatta aagtaaaaaa caggaaaaca attttaaaaa
     1741 atataaaatg taaagtttat aggaggattt aaaaaatgga aaaagtagtt agtatcaaaa
     1801 atgcaagtgg attacatgct agaccagcag gaatgtttgt taagaaggca gctgaattta
     1861 aatcaacagt agaggttgta gcaaaagaca aaactgtaaa tgcaaaatca ataatgggaa
     1921 taatgagttt aggtttagct caaggagaag aattaaagtt agttgtaaat ggagaagatg
     1981 aagaagtagc tttagctgca atggttgaat tagttgaaag tggatttgga gaatagatat
     2041 aataattttt taaaatttga tatttagaat ctaatattac ttaaagtaaa gtaacagaga
     2101 agtattaaac atatacagac caacatttgt agatatttaa tttctttgtt actttcatgt
     2161 ttatttaatc agaagattaa gactattgtt aattgggtat aaaaatataa atattttaga
     2221 aaatgaaata agatattttt aatcataaat tcagagtaaa aatacagtaa caaacagttt
     2281 ataaaattta ataaaaaatt ataagtaaat aaaactataa aaatgtaaat tatttatata
     2341 aatttataga gtttatgtgc tagatgatat tttttaggag gtagggttat ggcttacaaa
     2401 ggaataggtg cttctcctgg tgttgcttta gggaaagcgt tagtagttga acatagtgaa
     2461 ttagtaattg aaaaaaaatc tatagataat gttgaagctg aaattgctaa attagaagat
     2521 gcagttgcag tttctaaaga agaattagta aaagttaagg aaaaggcttc tgaagaatta
     2581 ggagcagagg aagcagaaat atttgaagca catttattag ttttagaaga cccagagtta
     2641 attggttctg caatagataa aattaaaact gaaagtgtaa atgcagagta tgctttaaat
     2701 gaaataaaag agatgtttgt tagcatgttt gagtctatgg acaatgagta catgaaagaa
     2761 agagcggcag atataaaaga tgttacaaat agaatactta gacatatatt aggaattaaa
     2821 gttgttgatt tatctgcttt aagcgaagaa gttgtattaa tagcacatga tttaactcca
     2881 tcagacacag caactatgaa taaaaaaatg gttttaggat ttttaactga tataggtgga
     2941 agaacttctc atactgctat catgtctaga actttagaaa tagctgcaat cgttggatta
     3001 aatgatataa cttcaaaagt taaggatgga gattttgtag ttttcaatgg agacacaggt
     3061 gaagtaattg tgaatcctga tgaagagact ataaataaat atactgaatt aaaagctaag
     3121 tatgaagatg agagaaaagc attacagcta ttaaaaggaa aaccatctgt aactttagat
     3181 ggaaaacatg tggaattagc tggtaacata ggaacaccta acgatataga aggtctaata
     3241 aagaatgatg ctgaaggtgt tggtctttat agaacagaat ttttatacat ggatagagat
     3301 tctttcccaa cagaagaaat acaatatgaa gcatacaaag ctgtattaga aggaatggat
     3361 ggaaagccta tagttataag aacattggat ataggtggag ataaagaatt aagttattta
     3421 agtatggaac ctgaaatgaa tccattctta ggttacagag caataagact ttgcctagat
     3481 agaaaagata tatttaagac tcaattgaga gctttatata gagctagtat tcatggaaga
     3541 ttaagaatca tgttcccaat gatttcatct ttagaagaat tcttacaagc taaagaagtt
     3601 gtaaaagaag ttttagcaga acttgattct gaaggtgtgg cttatgctaa agatgtagaa
     3661 attggtatga tgatagaagt tccatctgct gctgtaatat ctgatgtatt ggctaaacac
     3721 gtagatttct tctctatagg aactaatgat ttaattcaat acacttgtgc agttgataga
     3781 atgaatcaaa agataagtta tctttacaat caatttaatc cagctgtttt aagacttata
     3841 aagactgtta tagacaatgc acataaagaa ggaaaatggg caggtatgtg tggagaatct
     3901 gccggagacc aaaaaatgat accaatactt ttaggtatgg gattagacga gttctctatg
     3961 tctccaatat caatacttcc tgctagaaag ttaataactt cagttaaaga gtcagatatg
     4021 aaaaaattag ctgatgatgt acttaatatg ggtactgctg aagaaataaa aagctatata
     4081 gaaaagactt ttaatatata attaatgtta tatagttact aaaattcatt tttctaaata
     4141 acaaaattat attttacgaa ttaaaaagga gatgtttcaa aataaaaatt tgaagcatct
     4201 cttttttata aaagattact aagaattact taatctttgt aatagtcatt ttgtgaaaaa
     4261 gcaaaactgc attgtga
//
LOCUS       DAELSY010000226         4233 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.228, whole genome shotgun sequence.
ACCESSION   DAELSY010000226 DAELSY010000000
VERSION     DAELSY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.228"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            495..1160
                     /locus_tag="KPG09_003625"
     CDS             495..1160
                     /locus_tag="KPG09_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBG0968182.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            1182..2114
                     /locus_tag="KPG09_003626"
     CDS             1182..2114
                     /locus_tag="KPG09_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBG0968183.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            2135..2776
                     /gene="pcp"
                     /locus_tag="KPG09_003627"
     CDS             2135..2776
                     /gene="pcp"
                     /locus_tag="KPG09_003627"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBG0968184.1"
                     /translation="MKILLTGFDPFGGELINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIK
                     GGFIHIPYTTSQVINKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(2895..3227)
                     /locus_tag="KPG09_003628"
     CDS             complement(2895..3227)
                     /locus_tag="KPG09_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0968185.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            3493..4113
                     /locus_tag="KPG09_003629"
     CDS             3493..4113
                     /locus_tag="KPG09_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0968186.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKEAI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
BASE COUNT         1596 a          567 c          637 g         1433 t
ORIGIN      
        1 tcaaaatagt ctttaaactt tatcaaaatt ttatttttct tagtgaaatc taatgaaact
       61 agctcttaat atatgcttta ttaatcataa caaaaagaga gtgtctaatg aactaaaaag
      121 ttcattagac actctctttt tgtatgaaat caaatatatt tttatcattt caaaagagaa
      181 aaatacactt gtctctatgt ttgacacatt ctctttttat aatataatat ctaatttaaa
      241 acctatttca aatattgaca tatacttaaa ccatagactt acaaaatcaa ttcatattag
      301 cttaaaatat aatctttgtt actcaaattc ctatacacaa ccattattta ctttgatact
      361 ttaaaacatc ttccctattt tttattaaat tttataataa tagtcctatt aacgcaaata
      421 ctataaaaag agtttaactt tatccattaa ataaatttaa attaaaacat atagaaatgg
      481 aaggagaaca atttatgata aaactaatcg gtatattgat agttgtaatt ggatttatac
      541 ttaaaattga cacattattt actgtattat tagcaggagt ggcaacagga attgttgcag
      601 gattagattt taatcaaatt tttacaattt taggagattc ttttgtctca aacagaggtg
      661 tatcattatt catattaaca ctgccagtaa tcggtgtatt agaaagatat ggtttaaaac
      721 aaagagctgt ttctttaata gaaaaattaa aaagacttac aacaggaaaa gtacttacta
      781 tatacatgat agctagacaa atagctggtg ctctatcaat aagaatgagt ggacatcctc
      841 aatttgtaag accacttgtt aatcctatgg cacaagctgc tggtttaagt aatagtgatg
      901 aactaaaaga atcggatgaa gaagccataa aggcattatc tgctgcatca gaaaactatg
      961 gaaattttta tggtcaaaac ttatttgcag gtagtagtgg agtattactt atagcatcta
     1021 ctctaactca atttggatac aatgtaactg gacttaatat tgtaaaggct agtactatta
     1081 tggcagtaat agcacttgta gtggctacaa tccagtttac cttatatgat aaaaaattaa
     1141 acaaaagtca taaaaaatag ttttatttag gaggaataaa aatgcaaaat tttataaaca
     1201 tatcattgga aatcttttac gcactaatgg gatttttaat gattgttata gcatataagt
     1261 cttttactac cataaataac aataagaaat atggtacatc attattttgg atacttatat
     1321 cactgccatt catatttggt agattgattc cagccaatat aataggaata atattaatat
     1381 tatctagttt attaacactt tctaaacaag ttgtatttgc aaagtatgaa gaaccagatg
     1441 aaaattttgg aaaagaacaa gcagacaaac ttaaaaataa aatatttata ccatcattaa
     1501 tattagcatt tgctgcagta gtagtagcta tgagcctttc taattttgat aattcttctc
     1561 aatttgcaat aggtgtgggt tcaataattg cattaatatc agcccttata ataactaaag
     1621 caaaacctgc tacatcagtt caagatggct caagattact tcaacaaatg ggacctgcta
     1681 gtatgttacc acaactttta gttgcattag gagctttatt tactcaggct ggagtcggcg
     1741 aagttatttc aaccatgata tctggtgtag tccctgctga tagcagatta tttggtgtta
     1801 tagcttatgt attaggtatg gttatattta ccatgataat gggaaatgca tttgccgcat
     1861 tttcagtcat aacggcagga attggtattc cttttgtatt gtctcaaggt ggaaatcctg
     1921 ctataattgg agcattagca ttaacagcag gatattgtgg aactctactt actccaatgg
     1981 ctgctaattt taatatagtt ccagcagcct tgctagaatg taaaaatgat tatattgtta
     2041 taaaatatca agctccagtt gctttggtat taattatagc ccacatatta gtaatgtact
     2101 ttttaggatt ttaatataat taggaggtaa aataatgaaa attttgttaa caggatttga
     2161 cccatttggg ggagaactaa taaacccagc tcaagaagca gttgaaaggg tcaataataa
     2221 tataaacggt gcagaaatca taaagataac tataccaaca gttatgacaa aatctgttga
     2281 agctatagat aaagctattc aagagcacaa tccagatata gtaatatcag ttggacaagc
     2341 tggaggtaga tttgacatta cacctgagag agttgctata aatatagatg attttagaat
     2401 aaaagataat gaaggtaacc aggttataga tactattata aaagaagatg gtgaacctgc
     2461 atatttttca aaattacctg taaaagccat ggtaaaacat atgaatgaaa ataaaattcc
     2521 agctagtgtt tcaaacactg caggaacatt cgtttgtaat catgtgatgt atggaatcct
     2581 ttatatgata gacaagaaat atcctaatat aaagggtgga tttatacaca tcccatatac
     2641 aacttcacag gtaataaata aaaaaaatac accattcatg tctttagaag aaatagtaaa
     2701 aggtttagaa ttagctatag aagcttgtat tatatacaaa gaagatgtaa aagaaattgg
     2761 tggtgaaatt tcataataaa ttttttagta ttttataaat taaaaatact aactattaat
     2821 ttaaatatag actgtgagta gattattcaa acaattttac tcacagtctt tttatggcat
     2881 ctacagatac aaaattaagg tctattcctc tctccccact cacacattgc atctaaaatt
     2941 ggaatcagag attttcccct ttctgataaa ctatattcta ccttaggagg tatttgagga
     3001 tattcagtac gaataatcaa gttatctgct tctaattctt ttaatgataa actcaaagtt
     3061 ttataggaaa tagtatttat ataacgtttt aatgcattat atctgacaac ttcaaattct
     3121 acaagacagt aaagaattat cattttatat ttaccactaa tcagtgaaag tgtataacta
     3181 aatcctgttt cacttaaatc tgtatttgat atacagtcta tactcatata ttacactctc
     3241 cttttggtta gtattgaaca aaaatgtgcg tacttgtttt tagaataatt tataataaaa
     3301 ttatagtgta agaataattg aaagtcaatt ttataatagg aggtgtattt actaaaaaaa
     3361 gagttatttt aaataggatt ctaagattag acatttctat ataaattgat aatattcatg
     3421 aaaatcctat tttataaaaa ataatatctc ttagaaaatc tattaaataa aattcattgg
     3481 aggaaattta taatgaaagt tttattgata aacggtagcc caaaccaata tggatgtact
     3541 tatacagcac taaatgagat tactaaagta ttgagtaaac acgatattaa aacagaaata
     3601 ctgtatttag gtaaagaagc aatcccagga tgtattagtt gcgcatcctg ttttgaaact
     3661 ggaaaatgta ttcgaaatga taaagttaat gaattgattg aagatctcga taatattgat
     3721 ggtattatta ttggttctcc tgtttacttt tctagtgcaa ctggtcaatt aacttctttt
     3781 cttgaccgtc tatttttcat agctggaagt cgcatggcta caaaattagg tgcttcagtt
     3841 gtgtcatgta gacgtggtgg tgcaagtgca acatttgacc aactaaataa atatttttca
     3901 atcagcaata tgcctattgt atcatcacag tattggaatc aggtacatgg ctttactcca
     3961 gaggatgtta tgaaagatga agaaggatta cagactatgc gaactctagg agaaaacatg
     4021 gcatggcttc ttaagtgtat atcagcaggt aaaaaagctg gaattaaaga accacaatat
     4081 gaagaaacaa ttatgactaa ttttataaaa taaataaaaa atgcactaat attaattaaa
     4141 acaaaacact aatttataaa taaaataagt ataaatatag gataaaaaac actaaaacag
     4201 aggtattgaa ttttatatca aacctctatt tta
//
LOCUS       DAELSY010000227         4148 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.229, whole genome shotgun sequence.
ACCESSION   DAELSY010000227 DAELSY010000000
VERSION     DAELSY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.229"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            134..1336
                     /locus_tag="KPG09_003630"
     CDS             134..1336
                     /locus_tag="KPG09_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968187.1"
                     /translation="MSKKKPRIVAISFLIIVFGFFILNFTLPKKKLSITENRNLAKFP
                     TVENLKKCDFIAKFEEYFNDHFAFRDEMISINTRTQANLNKTKVGNYYLGKDNWILGM
                     FPEILDQKQLSRYEKSINYLSNLSKDMGKNVYFTMMPHKTNMLKHLYPKYVDNMDNID
                     INLKNFKNRLNPDLIKYIDMDEYFLSNYSDKERENLYFKTDHHWTGYGAFEGFKMMAS
                     NMDLGLSKQDLDKHFSSYKKSIITDKKFIGSYNQNIDMLIKENEYVVYMTKDNQDYSF
                     DINGEEKEEKEEKEVYATERDNKEWDYGGAYIRGAKTNILTIKNPKALVDKKILIFRD
                     SYQAPTTLLFADMFKEVEIADPRNINSIYKTYKEIIEESNADIVMFMYNSSGFDSMID
                     SMIDKGIK"
     gene            1537..2520
                     /locus_tag="KPG09_003631"
     CDS             1537..2520
                     /locus_tag="KPG09_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968188.1"
                     /translation="MKEVFNVHIMQSQNKKIKQKYYLTKDGWLLGNFAKLLSDYHIKS
                     YSKAINNLSKLNRKKDFYFVSLPHKTNMLKHLYPSGIEGKNNIDINKENLEKQLTDIN
                     FLDIDDYFYRSFTEKEREKFFLKTDHHGNSLGAFEEYKFILDKLDLGLSKSEIDSHLK
                     RYSTRYVNDKVFIGSYNKKMGYPLKKKESISYVEISDANYKYFISNGTKNTEINENII
                     KAKLKDKREWDYGGAYMRGCDCHILKIRNSSSLTNKKALIFRDSYQAPMTWMLADTFK
                     ELEILDPRYIKNIDMTYDEIIKNSNANVVLFMYNSFGFAGMIQEMIDKGIA"
     gene            complement(2970..3413)
                     /locus_tag="KPG09_003632"
     CDS             complement(2970..3413)
                     /locus_tag="KPG09_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968189.1"
                     /translation="MKKNMLYLGIVYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GICMIFQYMYWSKPERAVDYEEKIKNQRIEMNDERRVMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(3572..>4148)
                     /locus_tag="KPG09_003633"
     CDS             complement(3572..>4148)
                     /locus_tag="KPG09_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0968190.1"
                     /translation="IGLDLGIKEFCISSCGEFIENPKYFKKSLNKLAKLQRELSRKTI
                     GSLNRNKARLKVAGLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIKNRKLS
                     RLISDVSWSEFIHQLKYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWI
                     CPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         1649 a          543 c          529 g         1427 t
ORIGIN      
        1 attaaatagc acattatatc aagttactaa tgtggttttc attgtttctt gtatgtttat
       61 tgtaataata atgttaagta gctcaaccta taatccattc ttatatttta gattctaatt
      121 gggaggtata aacatgagta aaaaaaaacc aaggattgtt gccatttcat ttttaattat
      181 agtatttgga tttttcatac taaacttcac acttccaaag aaaaaattaa gtataactga
      241 aaatagaaat cttgcaaaat ttccgactgt agaaaattta aaaaaatgtg actttatagc
      301 caagtttgaa gaatatttta atgaccattt tgcatttaga gatgaaatga tatcaataaa
      361 tacaaggact caggctaatt taaataaaac taaggtaggt aactattatc taggaaaaga
      421 taactggata ttaggtatgt ttccagaaat tttagaccaa aaacaattaa gtcgatacga
      481 aaaatccatt aattatttat ctaatctaag taaagatatg ggaaaaaatg tgtattttac
      541 aatgatgcca cataaaacta atatgttaaa acatttgtat cctaaatatg ttgataatat
      601 ggataatata gacataaatt taaagaattt taaaaataga ttaaatccag atttaattaa
      661 atatatagat atggatgagt attttttaag taactattca gataaagaaa gagaaaattt
      721 atattttaaa actgaccatc attggactgg atatggagca tttgaaggct ttaaaatgat
      781 ggcatcaaat atggacttag gtctatctaa gcaagattta gacaagcatt ttagtagcta
      841 taaaaagtct ataattactg ataagaaatt tataggaagt tataatcaaa atatagatat
      901 gctaattaaa gaaaatgaat atgtagttta tatgacaaaa gataaccaag attatagctt
      961 tgatataaat ggagaagaaa aagaagaaaa agaagaaaaa gaagtttatg caactgaaag
     1021 agataataaa gagtgggatt atggtggtgc atacattaga ggagctaaaa ctaatatatt
     1081 aactataaaa aaccctaaag cattagtaga taaaaaaata ctaatattta gagattcata
     1141 tcaggctcct acaacacttt tatttgctga tatgtttaaa gaagtagaga tagcagaccc
     1201 tagaaatata aactcaatat ataaaacata caaagagata atcgaggaaa gtaatgcaga
     1261 tattgtaatg tttatgtata atagttctgg gtttgattca atgatagatt caatgataga
     1321 taaaggaata aagtagtaaa taaaaagcct ctattaaatt caaaatttaa tagaggcttt
     1381 ttattgttta ttttttattg ctttggtaaa aataaactaa tcaaaataaa agtaaggaga
     1441 tgtagcatat tgaacaaaag acatagtcta cctatagtat cagtgatatt attgatggtc
     1501 ttaattatct ttaataataa ccaaaattat attacaatga aagaagtttt taatgtccat
     1561 attatgcaga gtcaaaataa aaagattaaa caaaagtatt atctaactaa agatggatgg
     1621 ctattaggca actttgctaa attattatct gattatcata taaaatctta ctccaaagcc
     1681 attaataact tgagtaagtt aaatagaaaa aaagattttt actttgtttc acttcctcat
     1741 aagacaaata tgcttaagca cttgtatcca agtggtatag aaggtaaaaa taatatagat
     1801 attaacaaag aaaacttaga aaagcagtta acagacataa atttcttaga tatagatgat
     1861 tacttttata gaagttttac cgaaaaagag agagaaaaat tctttttaaa aacagaccat
     1921 cacggaaatt cattaggagc atttgaagaa tataagttta ttcttgataa attagacctt
     1981 ggcttatcta aatcagaaat agatagtcat ttaaaaagat acagtactcg atatgtaaat
     2041 gacaaggtct ttataggtag ttataataaa aagatgggat atccacttaa gaaaaaagag
     2101 tctataagct atgttgaaat aagtgatgca aattacaaat attttattag taatggtact
     2161 aaaaatactg agattaatga aaatatcata aaagcaaaat taaaagataa aagagaatgg
     2221 gactatggtg gagcttatat gagaggttgt gattgtcaca tacttaagat tagaaattca
     2281 agctctttaa ctaataaaaa ggcccttata tttagagatt cgtaccaagc acctatgact
     2341 tggatgttgg ctgatacttt taaggaactc gaaatactag atcctagata tataaaaaat
     2401 atagatatga cctatgatga gataattaaa aacagtaatg cgaatgtagt tttatttatg
     2461 tataatagct ttggatttgc tggaatgata caagaaatga ttgacaaggg aatagcataa
     2521 caatttgaat cagctccttt ttatacaaaa aatagttctc aataattcct aatctatatg
     2581 ataatttagt acatctcttt aacaataaaa ataaaatcac aaaaattctt ataccatcac
     2641 ttatctttat gataaattgg ttgagaaaaa attttataat ttatatgata tgcaatctta
     2701 attaattctt tatctactgc aacctttaat aaaagaaacc atccaagact attaatatga
     2761 atttatataa aaattacttt caaataaagt actgcagtat ttttatttaa agtatctata
     2821 atctttaacc acaaaaagcc acaaaataca atgtatactt atggcttttt ataaatttat
     2881 ataattgtat ataaagatat attctaaaac tgtcttaaga atctaaagtt taaaaatcca
     2941 atatttttat tgtgaattta aactttttat tacaactttt tatctaattt attgtataca
     3001 acaacactac aaataaattg aaataaaata agcaatccaa gaacaactaa aacccacttg
     3061 cttattacca ttacagaaaa tacactcaat atagaaacaa taaatataag aattacaaga
     3121 acataggaca tgatttgatt agtgatacgt ccagctttat cacgtaacat aactcttctt
     3181 tcatcattca tctctatcct ttggtttttt atcttttctt cataatctac tgctctttca
     3241 ggcttactcc aatacatata ttgaaaaatc atacatatac ctggacctaa agcagctcca
     3301 gataatcccc aaataaaagc ctcaaatgaa aactcagtaa acgtagccaa taaaacaagt
     3361 aaaattccac aaataaaata tactattcca agatataaca tattcttttt catttttact
     3421 cctcctcata aataaatatt tcttcaatac tcatctgaac ctcccatgac taaagtcaca
     3481 gggttcctgc ttcacagaat tttgcatact aaaatatgtc ttactaattc tccacaggcc
     3541 atccccgtag ttcctacggt tcttacatac gttatttatt ttgaattgtt attaatctta
     3601 atccttcttt taatatattt atacttgcat ttatatctct atcatgagtt tcattacaac
     3661 taggacaaat ccattctctt atatttaagt ccttaacttc ctcgttttta tacccacatt
     3721 tattgcatat ttgtgaactt gcaaagaatt tgcctacttt tacaatttgt ctaccatacc
     3781 aattagcctt gtattttaat tgatgaataa attctgacca tgatacatca gaaattaagc
     3841 gagatagttt acgattttta atcatattct ttacttgtaa atcttctatg caaataatat
     3901 cattctcttt tattaattta gtagatagtt tttgtagaaa atcatttctt tgattagcta
     3961 tatgttcttg aagtcctgca acttttaatc ttgctttatt tctatttaaa ctaccgattg
     4021 tttttctcga taattctctt tgtaatttag caagtttgtt taatgacttc ttaaaatact
     4081 taggattttc tataaattct ccacaacttg agatacaaaa ttctttaatt cccaaatcta
     4141 aacctatt
//
LOCUS       DAELSY010000228         4120 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.230, whole genome shotgun sequence.
ACCESSION   DAELSY010000228 DAELSY010000000
VERSION     DAELSY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.230"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            191..2290
                     /gene="nrdE"
                     /locus_tag="KPG09_003634"
     CDS             191..2290
                     /gene="nrdE"
                     /locus_tag="KPG09_003634"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBG0968191.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYLKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2303..3274
                     /gene="nrdF"
                     /locus_tag="KPG09_003635"
     CDS             2303..3274
                     /gene="nrdF"
                     /locus_tag="KPG09_003635"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBG0968192.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKKMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3350..3976
                     /locus_tag="KPG09_003636"
     CDS             3350..3976
                     /locus_tag="KPG09_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG0968193.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIIEIPEQKFIMLKGSGDPNSKEFI
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         1644 a          447 c          769 g         1260 t
ORIGIN      
        1 gtatatacta tatgtagtat ataatagata aagcactgac tatatgtagt gcttttgttt
       61 ttgaaaaata cttattgcat ttctataatt atagattaat tttgggattt caactgataa
      121 gtaccaaaag aaataataag caaattaagt gaaattatgg cacaagatta tgttaaaagg
      181 acggtaaata atgaattgga tagagcttaa caatcaagtg ataattaaaa atgaacaagg
      241 taagtaccaa ttggaaaagg ataaggaagc cttaagtagt tacatagaag aatttataca
      301 accaaagttg agaaaattta ataacctaga agaaagattg aagtatttaa tagatgaagg
      361 ttattattta aaagaagtaa taaatcagta tagcatagat gttattaaaa atatatatga
      421 tatgattgat aaacataagt ttgaatttca atcatatatg agtgcaaata agttttatca
      481 aaattatgca ttaaagagca atgatggaaa ggaaatatta gaaacatata atgataaagt
      541 gttaattgta gctttgacac taggaaatgg cgataaagat ttagctttaa acttggcaga
      601 taaaatagta aaacaagaat ttcaaccagc tacacctact tttttaaatg ctggaagaaa
      661 gagggctgga gaaatggtat catgtttttt actatctgta gaagatagta cagaaggtat
      721 atcatatgct atatcttcat caaatcactt atcaaagatt ggtggaggag ttgcactaaa
      781 tttatctaaa cttagagcat ctggcgaatc tatcaaagat atagaaggtg cagctggagg
      841 tgttgttgga gtagctaaaa tgttagaaca atcatttagt tactttaatc aaatgggagc
      901 tagacaaggt tctggtgcgg tgtaccttac tgtattccat cctgattttg aattgttgat
      961 ggatactaaa aagataaatg cagatgaaaa aataagactt gctacattat cattaggagc
     1021 tataatacca gataaattta tggagttggc agaaaaaaat gaagtagcat atgcatttta
     1081 tcctcatact gtgtataaga aatatggagt ttctcttgat gagatagaaa tggatgagtg
     1141 gtatgataaa ttggttaatg atagtgatat aagaaaaaaa gaaataaatc ctaggcagat
     1201 gttgacaaaa atagcacaaa tgcaacaaga aagtggatat ccatatgtag tgtacataga
     1261 tacagctaat agagaacata cattaaaaga tgttggaatt attaaaatgt ctaacttatg
     1321 ttgtgagata tttcaatatc aaacaccttc agaaatagag gggtatggag gaaaaaatga
     1381 atggggacaa gatataagtt gtaacctagg ttctttaaac atagccaatg taatggataa
     1441 taaaactata gaaagtacag ttgaaacagc aataagagca ctgtcatttg tagcagatag
     1501 cacagatata aaaccagtgc caacggtaag caatagtaac agcaagtctc attctatagg
     1561 attaggagct atgaatttac atggatactt agtaagagaa aatattttat atacttcaga
     1621 tgatgctata gatttttcta atgtattttt tgcaatggtt agatactatt caattaaagc
     1681 ttctatgaaa atagccatag aaagaaatca aacttttgaa ggatttgata aatcagaata
     1741 tgttaaggga agaaatagca aggttttatc aaaatactat gaacaatctt atctacctaa
     1801 gtcagaaaag gtaagagcat tatttgaagg gatttatata ccaacaaaag aagattggac
     1861 aaaattattg gatgaagtaa aagaaaaagg gatatataat gcatatttaa tggcagtagc
     1921 accaactcaa agcattagtt atgttcaaaa tgctacatca agtataatgc caattactga
     1981 gccagtagaa gtaagaacat atggagactc tactacaata tatccaatgc catttttaac
     2041 aaatgataat atgttgtatt atcaatcagc atacaggatg gatatgagaa aggtcataga
     2101 tttagtggca acagttcaaa accatgttga ccaaggtata tcaacaacac tgtttgttac
     2161 agatgaaaag acaactagag atatagctag acattacata tatgcctata aaaaaggttt
     2221 gaaaagttta tattacacaa gaacaaagat gacaagagat actcatgagt gtctagtgtg
     2281 ttcagtatag ggagagaaat atatgacatt taatttacat aaaatacata atgcagttaa
     2341 ctggaataga gaagaagatg gatttacaca agctttttgg gaacaaaatg taaaacaatt
     2401 ttggcttcct gaagaaatat ctgtttctaa agacataaag gtatggaatg aactaagtaa
     2461 taaagaaaag gaattatata aaaaagtatt gggtggttta acattactgg atacaaaaca
     2521 ggggaataat ggaataccat ctatgatgag tttaacagaa aacctacaaa gaaaagctgt
     2581 cttgtctttt atggggacta tggaagaaat acatgcaaaa agttattctt ctatatttat
     2641 gactctactt tctaatttag aaatagatga attatttgaa tggatagaaa cagaaccaac
     2701 attacaaaga aaggctgatt tagttttagc tcaatacgaa aatactacaa atcaagaggg
     2761 attataccta tcaatggtta caagtgtatt tttagagagt ttcttgtttt attcaggatt
     2821 cttttatcca ttgtatctat ctggtcaagg aaagatggta gctagtggag aaataatatc
     2881 gcttattctt agagatgagt ctttacatgg taagtatata ggattgctag cacaagaaat
     2941 atacgattca tttgataaaa tggataagaa gatgttagaa gagaagatgt attctatact
     3001 ttatagcctt atggagaatg agatagagta tacaaatgtt atatacagag aaagtgggct
     3061 tgaaaaagag gttgtgaact ttttaaaata caatgcgaat agagctttag aaaatctagg
     3121 gtttgagaag ctatatactg tagatgctat aaatccaatt gtattaaatg gtcttagcac
     3181 tgaaaccaaa acacatgatt tcttttcaac taagggtaat ggataccaaa aaggagtgta
     3241 tgaggaatta gaggatgaag attttataat ataatttata attatgacac actgttgtca
     3301 cttttagcgt gataatatca atgtataaaa tatatatgga ggtattttta tgaaatatga
     3361 atggagaaaa agagataaag agttatattt acctaaaaag aatcctgtaa taattgaaat
     3421 accagaacag aaatttatca tgttaaaagg gagtggagac ccaaattcta aagaatttat
     3481 tgaagcaata ggagtacttt attctttggc atatgcaata aaaaccatgc ctaaaaaagg
     3541 aattgtacca gaagggtatt ttgactatac ggtttttcct ttagaaggta tatgggataa
     3601 gggagaaaaa agtaaaggct tagatgtact tattaaagaa gatttaattt acactataat
     3661 gatacgtcag ccagattttg ttacacaaga cctgttggat aaagctactg atatagtaaa
     3721 gaagaagaaa cctcataatc ttcttgaaaa agttaggttt gggtctatta atgaagggct
     3781 atgtgttcaa atgcttcata taggttcata tgataatgaa ccagaaagtt ttgacattat
     3841 gaacgaattt tgcaaaaaaa ataaacttat aagaaaatct tacacacatc gtgaaattta
     3901 tataagtgat gctagaaaag tatcgcctga atcattgaag acggttttac gttttgaagt
     3961 agaaagggaa gtataaatat tttgaataat ttataagagt tataaaaagt gaagtctgtt
     4021 gagacttcat tttttgatgc aattttaaaa ttaaatttga tgtttcataa tattttaaaa
     4081 actttctact tttattagtg tgaataactg ttgctattaa
//
LOCUS       DAELSY010000229         4098 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.231, whole genome shotgun sequence.
ACCESSION   DAELSY010000229 DAELSY010000000
VERSION     DAELSY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.231"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..255
                     /locus_tag="KPG09_003637"
     CDS             <1..255
                     /locus_tag="KPG09_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0968194.1"
                     /translation="VSWSEFIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEV
                     KNLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            382..1284
                     /locus_tag="KPG09_003638"
     CDS             382..1284
                     /locus_tag="KPG09_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG0968195.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIATF
                     GKLLIPFIMAGILGIVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTSHIF"
     gene            1893..2906
                     /locus_tag="KPG09_003639"
     CDS             1893..2906
                     /locus_tag="KPG09_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG0968196.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKTFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPCDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFEIDLITTKSIFYPLSKQTPIENRTNSKPTYQINVY
                     IDN"
     gene            3262..3831
                     /locus_tag="KPG09_003640"
     CDS             3262..3831
                     /locus_tag="KPG09_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG0968197.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKSIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
BASE COUNT         1502 a          374 c          744 g         1478 t
ORIGIN      
        1 gtatcatggt cagagtttat tcgtcaatta gaatacaagg ctaattggta tggtagacaa
       61 attgtaaagg taggtaaatt ctttgcaagt tcacaaatat gcaataaatg tgggtataaa
      121 aacgaggaag tgaagaactt aaatataaga gaatggattt gtcctagttg taatgaaact
      181 cacgatagag atataaatgc aagtataaat atattaaaag aaggattaag actaataaca
      241 attcaaaata aataacttat gtaagaaccg taggaactac ggggatagcc tgtggagaat
      301 tagtaagaca tattttagta tgcaaaattc tatgaagcag gaaccctgtg actttagtca
      361 tgggaggttc agattggaaa tgtgttgcca caagattatt atggattaat agtgatttta
      421 ggtgttattc ttggagttat gggggcattt tacaactggt ttactctata tgtgcagtct
      481 ttatacaaga agattcctaa aattgctacc tttggaaaac tgttgattcc atttataatg
      541 gctggtattc ttgggattgt tatgcctgat gttctgggga gtggacacaa tttagttgaa
      601 gaacttacaa gtcatgaata tatttttgga atggttgttt tgatttttgt tatacgtttt
      661 atattttcgg ctgttagttt tgggtcaggt gcaccagggg gaatattttt tccattgctt
      721 gttttaggag catttatagg tggaatattt ggtatggtag gtgttaaagt ttttggaatg
      781 aatcccgatt atgtaaataa ctttgtgctt ttggctatgg caggatattt tacagctatt
      841 gtgcgtgccc ctttgactgg tatcatatta atttttgaaa tgacaggttc tgttagtcaa
      901 atgctttcac tatcagtaat ttcaattgtg gcatatattg tggctacact tatgaagtct
      961 aaacctattt atgaaagtct tcttgagagg ttacttgaaa aaaatggaga acctgtacat
     1021 aaagagaggg gcgaaaagat actagaccag ttctctgtta tgtttggctc tcctgttgcg
     1081 aacaaggcaa ttcaggatgt acagtggcct gaaaattgtc ttttggtagc aattcaaaga
     1141 ggtggagaag aaattattcc tagaggtaag acaattctta gaccaagtga tgtaattgtg
     1201 actatgacag atgagagaga tagtggtgct gtgtatgata agatggaaaa tttgtgtaaa
     1261 gaacatacgt cacatatttt ttagggataa tgatgaatgt ttaataatga tagaatattt
     1321 aatgaagatt tgattaaaaa attggtaatt tgaaagagag ttaaggaatt aatagaaaat
     1381 gaaaatgtga tatcttagat gtaaaagagt ttgaagaaaa taattgtata tatttgttaa
     1441 tgagattata tggactaatg gtatatttat actgtgctaa aagttgcact attgtatgag
     1501 gaatgggaat aaagggtttg aaaaaatagg tagaagattt ttcagatatt cttattggga
     1561 tattttaatt aatatatttt aattgcgata ttttgggaag aatagaattt atttataaat
     1621 gaaaataatt aaaaggggtt aaggttgtat tagccccttt tgcttataga gtttggaaat
     1681 ggtatcttga tagcagttgt tttatgctta ttatatttta ttacatattt taaaattaac
     1741 cttatcactc ctaaatcatt attctatcaa cttttaaaac actttcaaga caatcaccta
     1801 ttgaaaacaa agttaattaa aaacctacat atcagtttag tattcgtgta gatgattata
     1861 taaatataaa agtataagaa aggtgagtat ctgtggttag ttcattttta aatgatatct
     1921 ttagaacaat tttagtaagt tgtattttta tagttatttt attagtcttt agaataactt
     1981 tatttaagac tttcagtaaa aaatttaatt actatatatg gcttattgtg attattaagc
     2041 tatcattacc atttatgaat tatactttta tatttaatga gtcaaaatat aatggtaata
     2101 ttaataagat aagtttaggc aattttaata gtatttctat tgtatatagt actgttttag
     2161 tctctgtttg gattgctatt acgatatttt atttagttta tactttattg aaatatatta
     2221 agcttaaaaa tatgataaat gatttgtctt atgatgttga ggatgataag ataaaaaatc
     2281 tttatgaaaa tttattaaaa gagcttaaga taaataaaaa aatacggctt aaatattcat
     2341 atgaagttga aagtccagta ttttttaatt cgtgtgtcat tttaccacca tgtgattata
     2401 cattaaaaga gttaaactgg atatttaggc atgagttgat gcattttaag agtaaagacc
     2461 tttacattaa gtatttggtg atatttttaa aatgtgttta ttggtttaat ccttttgtgt
     2521 atataatgga gaagtttata gatttggatt gtgaatttta ttgtgatgaa agagttttga
     2581 agaattgtac gatagaagag aagcaggatt atgcattgac aataattagt gcaatgagaa
     2641 aaggttcaaa tagttcaaat aaatttgtgg ctggattaca taaggagtca gatattaaaa
     2701 aaagagtgtt tagtatgttt aatgaaaagt acagaaatgg tattttgata gcagttattt
     2761 tatgtttatt atcttctatt acatattttg aaattgattt aattactact aaatcaatat
     2821 tttatccact ttcaaaacaa acacctattg aaaatagaac taattcaaaa cctacatatc
     2881 agattaatgt ttatatagat aattaaaaaa tacaaagttt aacaagagat taatataaat
     2941 aattttaata agtatctttc acatatgtta aatttacttt aattatatta aagccaatga
     3001 attattcttt agaagctcca atgccagtaa atacaggtaa agaaaaatct tttactacaa
     3061 ttgtaaatta aaaaatattc taatatttaa agaaatgcaa aagtgtaaga agggtaaaaa
     3121 tctgttataa tacattaaaa actaaattta gaaaaattta cataattaga aaaatgattt
     3181 gataaaaaat aaagttaaat attaactaag taaatttgaa aaagaaaaaa tataaagtac
     3241 aaatttaaaa ggaggtaact aatgaaaaag aagttggaag atatgacttt agaggagtta
     3301 tggaacttgt ttcctatatt tttgactact cacaaagaat gttggaaaaa ttggtttaaa
     3361 gaagaacaat atagactaac tggaatattg ccaatgcaaa aaattgttag aataagtcat
     3421 attggaagta cttctgtgag tacgatttat gccaagtcca ttgttgacat actgattgag
     3481 ataattcctg attataatat gaaagatatt aaagatttga ttgagtcgaa tggatatatt
     3541 tgtatgtctg agagcagtag taggctttca cttaataaag ggtatacagt agatgggttt
     3601 gaagaacgcg tttttcattt gcatataagg tatgtaggtg ataatgatga gttgtttttt
     3661 agagattact taattgacca cccagatgtt gcaaaggagt atgaaaaact taagttggac
     3721 ctttggaaga agtatgagca taatcgtgat gcttatacag atagtaaact agattttgtt
     3781 agggagttta ctgaaaaggc aaaggtgttg tattctaata gatataatta gaaataggta
     3841 caattagata tttatcttat atatcgtatt atatttttta agaaagtaat taatagaaaa
     3901 aggggttgtt tcaaaatgga gagataaatc cctttttcat tgttgaaatg ataaaaatat
     3961 atttgatttc atgtaaaaaa gagtgcctca tcaactaaaa agtttatttt gagacacaca
     4021 catttttatt aaggtaaata aaaatatcac atttgattaa attttaagtt aatgaatata
     4081 tgtgtaaata tttaatta
//
LOCUS       DAELSY010000230         4088 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.232, whole genome shotgun sequence.
ACCESSION   DAELSY010000230 DAELSY010000000
VERSION     DAELSY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.232"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    336..574
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            638..2557
                     /gene="thrS"
                     /locus_tag="KPG09_003641"
     CDS             638..2557
                     /gene="thrS"
                     /locus_tag="KPG09_003641"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBG0968198.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            3325..3753
                     /locus_tag="KPG09_003642"
                     /pseudo
     CDS             3325..3753
                     /locus_tag="KPG09_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1594 a          445 c          756 g         1293 t
ORIGIN      
        1 tttaagtatt tactgtacta cttattaact atattaagta ttgattgtat ttaagtatac
       61 aaaacctaaa aagagtacaa aaattagaag ttagttttaa taaaaattag ttttagtatt
      121 tatctagtca tatctatcat gcatgtggat acgttttaaa aattttaaat ttatgaaaat
      181 attataatat cactataaat caagagatta atttaatata aaaatataag aatattggtt
      241 tacagctgat tgagatgaaa aataatgggt aattaatata ttacattaat gttgattgat
      301 gtataactat ctgttataat gaaaatatat taaaaatcat agtgttgaag aggaagagta
      361 gagtagaaac cattctcaga gaatttgggt ctatggctga gagcccatgt aataggtcta
      421 tttgaaaact accttggagc tgagcttatt agcagtattt gtatttgtac tatacgtgaa
      481 agtgctcggg tgacgacctt atagtcatca agagggttgt atatacttgt atacgccaac
      541 ttgggtggaa ccgcgaatta atatcgtccc aaacatattt ggggcgattt tttattacaa
      601 aaaatctaaa aattatttca ataaggagga aaaaacaatg attaaagttg ctttaaaaga
      661 tggctcaata aaagaatttg aaaatgctat ttctgttatg gatgtagcta aatccattag
      721 tgaaggatta gctagaaatg tggtagcagc gtctgtgaac ggagaggttg taggtctgga
      781 ccacattata gatactgatt gtgacttgaa tttatttaag tttgaagata aagaaggtaa
      841 ggaagttttt agacatacat cagcacatat attagctcaa gctataaaaa gactttaccc
      901 agaagcaaag cttgctatag gaccaagtat agaaaatgga ttctactatg atatagattt
      961 agaccataga ttagttccag aggacctaga gaaaatagaa gctgaaatga aaaaaatagc
     1021 taaagaagat ttaaaaatag aaagatttga acttcctaga aatgaagctt tagagcttat
     1081 gaaagaacaa ggagaagact ataaggtaga acttatatct gatttacctg aaagtgaaat
     1141 aatatctttc tacaagcaag gtgattttac tgatttatgt agaggaccac atttaccatc
     1201 tactaaaaag gtaaaagctg ttaaattaca aagtgtagca ggtgcatatt ggcgtggtga
     1261 cgaaaataat aagatgcttc aaagaatata tggaacttct tttgagaaaa acaaagactt
     1321 agaagagtac ttacatttac tagaagaagc taaaaagaga gaccacagaa aattaggtaa
     1381 agaattagga ttatttatga tacctgaaga aggtccagga ttccctatgt ttttaccaaa
     1441 aggtatggaa cttaaaaatg aattattaaa attctggaga gaaatacaca gaaaagctgg
     1501 atatattgag atagaatctc caatcatatt aaatagaaaa ttatgggaga cttcaggaca
     1561 ctggtatcat tacaaagaaa atatgtatac agttaaaata gatgatgagg attatgctat
     1621 aaaacctatg aactgtcctg gtggattaat ttactataac tctcaactac attcatatag
     1681 agatttccca atgagagttg cagagcttgg tagagttcat agacatgagt tatctggtgc
     1741 attacaagga ttaatgagag ttagagcatt tactcaagac gattcacata tattcatgtt
     1801 accagagcaa attaaggacg aaataaaagg agtagctaac ttaatagatg gtatctacaa
     1861 aacttttgga tttgaatata atttagaatt atctacaaga ccagagaact ctatgggaag
     1921 cgacgaagag tgggaagcag cagaaaatgg attaagagaa gctttagaag agttaggtct
     1981 accatatact attaatgagg gtgatggagc attctatggt cctaagatag acttccatct
     2041 aaaagattgt ttaggaagaa cttggcaatg tggtactatc caattagata tgcaacttcc
     2101 aagacaattt gataatactt atattggtca agatggtgaa aagcatagac cagttatgat
     2161 tcatagggtt gcttttggta gtatagaaag atttataggt atattaatag agcattatgc
     2221 tggtaaattc ccagtttggt tatctccaac tcaagttaag atacttccta tatctgataa
     2281 atttatggat tatgcaaatg aagttaagaa agagttattt gataagggta taagagttga
     2341 attagatgat agagctgaaa aaattggatt taagataaga gaagctcagc ttgaaaaagt
     2401 gccatacatg ttaatagtag gtgaaaaaga agtagctgat aataatgttt ctgttagatc
     2461 aagagataaa ggtgaaatag gtagtattaa attagatgaa tttatagcta gtatttcaaa
     2521 agaaattgaa tcaagagaaa gtattattca agactaatat ataatatatt agtagctaag
     2581 atattgttat ataagataag tttaatcaat atgttgatat gttgatacat agtgattaaa
     2641 tttatgaaaa gatgagtata attagaggta cagttaatca tatttttatg attaatgtac
     2701 ctctagtttt ttgtatggta gatttattaa taaatatatc atagaagtgt tttgaacatg
     2761 gaaatattta tgtgcattct ttaaataaaa gtttattttt tgaatgataa ttaagtgctt
     2821 gttggtaagg ttatggctgt aaaaatcata actttatttt tatatttgtt ccattttcat
     2881 aaaaattatt atccctttaa gaaaatttct taatcctctt gaaatatttc ctttttagtc
     2941 taaagttaaa tttctttctt caaaatgaat atttaaatga actaaaattt aagtacattt
     3001 tctaatctta tgagtctatt tttcatagat aattgcccta taatagaaga atctatgttg
     3061 gggctatgtg gtgacaataa cagcattgtc agtgattaat taatgcttta taaatgatta
     3121 attagtgttt tatgttattt ttggacttac cctctataca tagattaatt tatttaactt
     3181 tagatataga gagaatgtca agtacataca cagacattag agtttaagaa agtgtttatg
     3241 gagcaagaac ttttaaaaga agtagtgact tagtttaatt tcaagaaatg ataaatacaa
     3301 aagaggaaaa agagaggtgt tgaaatgata gaaaaagtag aagaaaaatc taagattatt
     3361 aaaacatcaa aagaatataa gcgaactata attgaagaaa aagataaaga atggaccaaa
     3421 aaattaaatg atatacttag tagaagtagt ttttgtaatg atgaattaga taaaatcaag
     3481 gcgttaattc caaaagagat tttgactaat gaaaatgttg gagaagttgt gactgaagat
     3541 ggatatgcta agcccgagta tgatgaggta tttaaatcat tgttcacttt aaataatgaa
     3601 tatgatttac ttgtaaattt tataaatgat attctaaaag atgctccata tgctaacagg
     3661 aatatcaaac cgttcactca aataaagaga ataattagag ttgaaactga tccaacaatt
     3721 aattacattg gagagaaaag acctagactt gattaaaaaa tagctacgca cacttgtgac
     3781 tttagtcatg agttagtagc taaaatagtc agcatatagg gaaacttata tgtagagata
     3841 ggatagaacc tatccaatac tacttgaatt gctaaaaacc cctaaagcta accaaactaa
     3901 aacataagga tgaaataaac ctaagtgtga aagttacgaa agtagaaaaa attggttaga
     3961 tggtataagg ttaaacccta agtactttaa aatgggcaat cagcaggtaa gctccgaata
     4021 ggagaagctt caacgactat tcctcttgag ggaagtacac tacaagttat tggtagtgga
     4081 agtgggta
//
LOCUS       DAELSY010000231         4072 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.233, whole genome shotgun sequence.
ACCESSION   DAELSY010000231 DAELSY010000000
VERSION     DAELSY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.233"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..896)
                     /locus_tag="KPG09_003643"
     CDS             complement(<1..896)
                     /locus_tag="KPG09_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBG0968199.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIET"
     gene            1158..1535
                     /locus_tag="KPG09_003644"
     CDS             1158..1535
                     /locus_tag="KPG09_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0968200.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            complement(1691..2140)
                     /locus_tag="KPG09_003645"
     CDS             complement(1691..2140)
                     /locus_tag="KPG09_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0968201.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(2318..2722)
                     /locus_tag="KPG09_003646"
     CDS             complement(2318..2722)
                     /locus_tag="KPG09_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBG0968202.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            complement(2835..3701)
                     /locus_tag="KPG09_003647"
     CDS             complement(2835..3701)
                     /locus_tag="KPG09_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG0968203.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMSPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRSTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
BASE COUNT         1347 a          681 c          448 g         1596 t
ORIGIN      
        1 gtttcgatta tctcactata aggcaataaa gtcgcttcga ttattgattt atttgtcttt
       61 tctaaataat acttaatcat ataatatcca caagcatatc ctgcacaata aggcaaccct
      121 actggaaaat atccttgcat tttagctata tcatcaccat aaagataaga tgttatattt
      181 tgaaatccag tttcttttaa agcacttttt attattggct ttatataagt atttaatgtt
      241 tcgatatctg ttctactgac ccaaggtcct aacatttcct ctccaaacat ccatgttgca
      301 aaattttctg caagaccttc atttatcatc atttcttcta atgttatatt attattccac
      361 tctataaact gaaatctaat attgtgatta cattcatgtg ctattaatac tggtaatcta
      421 ttgatagtat attcattagg aaccaatgat agaaatatat atccaggaat cccaccatca
      481 ccccagtatc catcacttaa tattgtataa ggactatttg gattcgccaa taatattgaa
      541 tatttatagt ccttaatgtt taaatcatac ccttctttta taaaagcatt tatagagttt
      601 tctatggttt ctttgcaagt gccccaaatt ttatcagcag ataacacatt tatattcttt
      661 ttttgctttt catcaatatc cttaggcgat aaaaccccta acatttcact tgctataatt
      721 acatcatatc cacctttctg tctagcaact attggaacat tcatacactt ccacttaaat
      781 tcaaaaggct tcatcaattc atatctgtat atatcttctt ttttgtttat tggtgcatcc
      841 attatcttct tgtatatttt atctgagcga atttctataa tattcatatt taccatctcc
      901 tttaagacaa agtatataca attacgtagc gtcaatgtca atatacattt tttataatgt
      961 caccttaatc tttttattat attttatcct gtaaaccttc cgtacatata acattttttt
     1021 aaattaataa atttgtataa tataataata aaaaattata tacttcaagg tttccctatg
     1081 atacaattta ctaaatcata atttagatta tcaggtgcaa ctataaatta ttctatatca
     1141 aggaggatat attggtcatg ctaactccat atttaatatt taatggtact tgtgaaaaag
     1201 catttaattt ttatgctgag gctttcggag gaggaaaaac tatatttgcg cgattagaca
     1261 gcaatccaaa caatcctatt atgcacgcaa gtgttacttt cacaaaatac gaaggttgta
     1321 taatgggtgc ggatacagac aagcctgttg taatttctgg catggcgatt tgtgttgttc
     1381 taccatctcg agaagcgata gaagaaatat ctgtaaaact tgccgagggt ggtacacttg
     1441 tacaagaatt tttaccacac ccaccaccac atcaaaatga tggcgctgct gaagtacttg
     1501 ataggtatgg gtatacttgg tatttaagta catagtaaaa ataaaaaaac tacatctaat
     1561 aatcataata tagaacagat aaatttcaat ttaatagtgt agtaaaaaag tcgtgtccta
     1621 agactgtcac gacttttatt tgtataaaaa tttaagtaaa tatttttgcg aataagtgag
     1681 aagtataact ttaaattaat ttcatatgta gtattggaaa tgggttacct tgtccatcta
     1741 tttcagacct atcaaacact ttaaatccta agtgaatata aaatcctaca gcttttttat
     1801 tctgctcatt tacatcaaca tattttacat tgtatttatc tatagcatat tttattaatt
     1861 ttttaccaat accatttcct ctatatttat cttctataaa gagcatctca attttatctt
     1921 tatctattcc tataaagccc tttattatat cattatcttt tatacaaact atattttcta
     1981 cttctttaag tccaatttca actaatggta tcaaagattc aatatctttt tctgttaaaa
     2041 aatcatgcgt tgctcttact gatgattccc agacttgcaa catttcgtca tattctgttg
     2101 aatttatttg tttcatttca agaattattc cagatttcat aaaatttcct ccctatatca
     2161 cttttaattg tataacactt ttttatacaa aacttagaaa ggactacgaa ggttaagtat
     2221 catagtccta attaagttta aatattatct attatatatt tttaacattt attatatact
     2281 ataatctagc aaatttacta caataatcca tatcagttta tactaaaggt gcttctattt
     2341 gagtagtaaa aaggaattta tgacaatgac catcattaac agtagtttct ccacatatta
     2401 agtgaatgtg tttatcagtt ccaggtattc taattgctgg ccctgttgta acacatatct
     2461 tatggaaatg gtcaaggaaa tctacattat catttatttt atgaacatga tttgtacctc
     2521 catttattgg tatagcttca ccagtaaccc ctgctgcacg gtggttatgt ctatcctcac
     2581 attcttctgc taacttaaca ctttctgaat attcatgaac atgtgtcatt tcctcataat
     2641 cattatagca cttgttgcac ttattgtatt tataatcatc atacacataa tctctttcac
     2701 atttataatt ttgataactc atttttatat ctctccctat attatattaa gaaattcttg
     2761 taaagaatct ctaatgtata atatgaatta aagtcaaaaa agttcatatt ttgtaaaatt
     2821 atgtattaat ttaattattt ctcccagtat ttagggcgta ttagtgaagc tggtaacttc
     2881 gtactagaat ctccttttgc agtatttact tgcaattgtg ttaaaaatat actattttca
     2941 agattagcac ctctaatatc acaatctctt aagtctgctc ctattaaatt tgccccactc
     3001 aaatctgtac ttcttaaatt tgctgctatt agaaatctac ctcttaaatc tgctcctatt
     3061 agatttattt ttctaaggtc tgctcctatt aaatctatat ttttacttaa ttttttcata
     3121 tttttaagag tagaagtctt gataaatgat ttaacctttc ccatcacaga ctcactcgct
     3181 tgtgataata ataaattaac ctttgttcta tgtgatatga tatccaaatt aattatctgt
     3241 tctgggctca tattagttat cttttcagtt tcttcaatta tcaaatcaat ttttgatttg
     3301 attgtatctg atatatttaa tacagatgct tctttaagat accataacat ttcatgtaat
     3361 tgtctcataa ttaaaaacac ttcagacatt tgatttgtta attctggtga ttgcatccag
     3421 tctatcccct tatatgttac ttgtgctact ttttgacctg aaccaaaaca atcatatgca
     3481 gtacaccctt taaaacctcg cttcataaga ctattatgta cagaacattt aaaatctggt
     3541 tgtaaattaa tacagggttt tcctgattcc ttatcgatag gaaatccatc tgaagcagaa
     3601 aaataaagag ctacacaaca aagtccaaaa cacttcttac aatcaatctt tagctcctca
     3661 aataacttat tatcatatat atttttatgt atatctatca tattttttct cctcttaagc
     3721 tttattttta atattatatt atctataact aaatgataat caagtccctt atttaacagt
     3781 tcttattctt aattataatt taaggtatta tttattccaa tagcatacat aagtttcttt
     3841 atacttgtaa caataccttt atactaagac ttattttaaa tgtagcactg taataataaa
     3901 ttgtttatat actcataatg tatttgataa attgtaaatc tttatagaag ttgaaatttt
     3961 taatgatttt agaatcaaga tataataata aatacaattt ataaaatcag aaatatataa
     4021 attaactata caaacctaaa taacatagca tttaaattca caatttcagt at
//
LOCUS       DAELSY010000232         4057 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.234, whole genome shotgun sequence.
ACCESSION   DAELSY010000232 DAELSY010000000
VERSION     DAELSY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.234"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            92..1495
                     /locus_tag="KPG09_003648"
     CDS             92..1495
                     /locus_tag="KPG09_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0968204.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFMLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     TSLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1586..1826
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1869..2567
                     /locus_tag="KPG09_003649"
     CDS             1869..2567
                     /locus_tag="KPG09_003649"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBG0968205.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILKGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2590..3591
                     /gene="trpS"
                     /locus_tag="KPG09_003650"
     CDS             2590..3591
                     /gene="trpS"
                     /locus_tag="KPG09_003650"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBG0968206.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANAIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
BASE COUNT         1526 a          452 c          732 g         1347 t
ORIGIN      
        1 tgaaaaatat cagtttaata ataatgaatc tattttatta attttgaaat taaatttaaa
       61 ataattaata gagaggggaa atgaaattag gatggaaaag aaaaaaatag gcctatggag
      121 tttggtattt atgaatgtat cagctttata tggtataaga tggatagcta agtctacagc
      181 atcaagcttt ggtttaggat taggtgcaat acctatgtgg ttattattct catttatata
      241 ctttgtacca ggagctttaa tttgcgctga attagcttct acatatcaaa agggtgatgg
      301 aggtctttac gattgggtta aggaagcctt tggagaaaag tatggatttt tagtttcttg
      361 gttaaactgg acagctaaaa tattttggta ttcttcattt ttaactttct tagcagtaaa
      421 tgtttcttat acaataggaa atccagctct tgctgataat aagatgtttg tacttatatt
      481 atctttaata atattctggg cattatctct tatagcaact aaaggaataa gttttacaaa
      541 aatatttact aatacaggtg cattaggttc aacaatacca agtgctatac ttataatatt
      601 ttcatttgta gcagtattta tgttgaaaaa acatgatata gcttctacat atacaatagc
      661 ttcaatgact ccaaaactta atatggatgc atttgttgct atatcagcta taatgtttgg
      721 tttagcagga gcagagactg ctgctaactt cataacagaa atagataagc ctgaaaagaa
      781 cttccctaag gcaatattaa tatcagctgg aatagtagct tcattatatg tattaggttc
      841 aattgcaata acaatgataa taccaccaga ccaaattact gcttctaaag gtgtattaga
      901 tgcactttct gctgtttgta cgtctttagg aattggttct tggttggttc aattaatagc
      961 ttttggtata gctttctcag tattaggagc aatagttctt tatatagcat caccaataaa
     1021 aatgttattt ggtagtgtta aaaaaggaat atttccagat agtttggtag aggtaaatga
     1081 acacaagata ccttctaaag cagttatact tcaagcaata atagttacta taatactatt
     1141 ggtgactaca ttaatgcctt ctgttgatgc tatatacaat gttcttgtaa ctatgacagc
     1201 attaactgca ttattcccat atgtattatt atatgcttct tatattaaac taagaaaaga
     1261 gagaccagat gaaattagac cttatactat ggcaaaatca acaagtactt gcctaggttt
     1321 agcaaaaatg gttttagttg taactgtagt tggtatatta ttatctgcag caccagttat
     1381 gccgacattt gctgaaaatg ttatatatga gatagagatg attggtggag gtcttctagt
     1441 tatattatct ggtctatggt tatggaatag atatgaaaag aaaacagcta aataaaatgt
     1501 attttaaaaa tttcttttgg atattataat atatagttga caatttatag tgtaaggaat
     1561 attataaaat aaagaaatta ataaattaaa tcctgagata agaaatagta aataaatata
     1621 tttttgcaca gagaattaag gatgctgaga acttaataaa aagtatttat tgaatgggtc
     1681 tttgaggagt aaaatgaaat gtaatttaag attacagagt atttgatatc gtgtatgcac
     1741 gttatagcat atattaagat atatatattc agttttgagt gtatataaat ttaggtggta
     1801 ccacggaaaa tatatccgtc ctattgattt aggacggatt ttttaatata gaaaatttag
     1861 gaggaaaaat gaacgtaata ggaattatag gtgcaatgga tgaagaagtt agtattttag
     1921 ttgatttaat ggatattaga gagactataa aaaaagctag cttagaattt tataagggaa
     1981 ttttgaaagg taagaatgta gtgttagtaa agtgtggtat aggtaaggtt aactctgctc
     2041 tttgtgccca gatactaata agcgaattta aggttgatgc tattgttaat acaggtgttg
     2101 caggagcttt aaatgaaaaa cttgatgtta atgatatagt aatatcaact gatgctatac
     2161 agtatgatgt agatactaca gcatttggag accctaaggg agttatacca agaatgaaga
     2221 cttctgtatt taaagcagat gaaagactta tagatgctgc ttacaaatct tctgtggaag
     2281 aagtaaagac tcataaagta ttaaaaggaa gagtagttac aggagataaa tttataaatt
     2341 ctaaagaatt aaaagaagaa ttagtaaatg attttggtgg atattgtgga gaaatggaag
     2401 gtggagctat agctcatgta tgctatttaa ataatactcc attcgtaata ataagagcaa
     2461 tgtctgataa ggcagatgga agtgctgatg ttacttatga tgtatttgta catgatgctg
     2521 ccaataactc taaagacata gttttaaata tgttaaaatc aatataaata tataaggagg
     2581 tacaaaaaaa tgagtgaaaa aaaagttata tttagtggag cacagccatc aggcaaaatg
     2641 actttaggaa attatttagg agctataaaa aactggactg aacttcaaga taattatgat
     2701 tgctattata gtatagttga tttacatgca ataacagtac ctcaggaccc taaagtttta
     2761 agagcgaatg ctatagaatt acttgctcaa tatatagctt gtggattaga cccagaaaaa
     2821 aatactatat ttatacaatc acatgtcaaa gagcatgttg aacttatgtg ggtattgaat
     2881 actatgactt atatgggtga attaagtaga atgactcaat tcaaagataa atcacaaaag
     2941 agtgaagcaa atctaaatgc aggtttattc acatatccag ttcttatggc cgctgacata
     3001 ttgttgtatc aaactgacct agttccagtt ggagatgacc aaaaacaaca tttagaatta
     3061 gctcgtgatt tagcaaatag gtttaataat agattttcac ctacttttgt agttccagaa
     3121 ggatactacc caaaaggtgg agcaagagtg atgagtttgc aagagccaac taaaaaaatg
     3181 agtaaatccg attcaaatga aaatgcattt atactattag cagatgattc agattctata
     3241 aagagaaaga taaaaagaag tgttactgat tctttaggtg ttgtaaaata caatgatgaa
     3301 caaccaggta ttaaaaatct aattgatata tattctaatt taagtaaaaa gagtgtagaa
     3361 gagattgtaa acatgtatga aggaaagggt tatggcatat ttaaagaaga tgtggctgaa
     3421 gtagtatcag aagctttaag acctataaga gaaaaatatg ttgaccttct taataataaa
     3481 gattatttag aaaaaatata tagtatggga gcagaaaaag ccgaaaaaca agctagaaaa
     3541 actcttagaa aagtttataa aaaagttgga tttatagaga gaagatacta attattagct
     3601 atctttactt gataaataaa ttttaaagtt attaaaaagg tatatgatat gaaagagaga
     3661 gtctcaaagt gaacttttgt tcatgagaca ctcttttttg tatgaaataa aatatatttt
     3721 aatcacttca acaatgaaaa agaggattat ctctattttg agacagcatc ttttgataat
     3781 aaaaaaagat gagcttttgc tcatctcaaa ggggttagat tattcttact ttggggaaag
     3841 tagaataaag gtaaaagtta actgatgtca actttgtctt ttttgcatat cactactgag
     3901 cataaaaaaa tcagaaaacc atagccttct gaaacaactt ctaacataca ttgtatttta
     3961 tacccaatat gtaatttatg agttaatttt aactcttata atggaaattg tcaataatat
     4021 atcaaaaaaa tattttttaa atttatataa ttaaatg
//
LOCUS       DAELSY010000233         4048 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.235, whole genome shotgun sequence.
ACCESSION   DAELSY010000233 DAELSY010000000
VERSION     DAELSY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.235"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            463..1494
                     /locus_tag="KPG09_003651"
     CDS             463..1494
                     /locus_tag="KPG09_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG0968207.1"
                     /translation="MKKIRKLATKLLITTIVLVSGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNNKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPTVGAVAQTQKPALPGKHTMLPHQDGAK
                     VVGKFGDIKDKHVMYEGVRCVVINSSVNTVTFLGSKSNLDPSNLTIAVIGFMNTPYGM
                     FALAPEGEPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIEITDEKANIGL
                     GQIMYIRYE"
     gene            complement(1610..2584)
                     /locus_tag="KPG09_003652"
     CDS             complement(1610..2584)
                     /locus_tag="KPG09_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968208.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYKLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNHPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            3040..3921
                     /locus_tag="KPG09_003653"
     CDS             3040..3921
                     /locus_tag="KPG09_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBG0968209.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSNKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
BASE COUNT         1516 a          533 c          665 g         1334 t
ORIGIN      
        1 aatcaaattt taagttcaac tcctttttta tatttttata gatatatcct atattttccc
       61 agaaaacgta ttgaatatac aaattataag aagtataatg ttgttgggag agaatgtatg
      121 aaatgtatac aaagttgatt ttaactgtat tattgataat tctctttact cttaccatta
      181 ttaatgattg aagacaatgg tatttattat aggtcaaaga ttccgaatat attaatcatc
      241 atcattttct caaactaaca gcaaacaccc tgatttgcga gttgtattgt atacaaatta
      301 tctagaaaat atctattaga ttttccatga aatttaaata tttcacgaac gtataaatga
      361 agaaacatac atattgacaa tccacctttt taaatcccaa atgaacttta ttaatggtag
      421 agaataggaa gtaacaatat tttataaagg agaatactaa gtatgaagaa aattaggaag
      481 ttagctacca agttattgat tacaacaatt gttttagtct caggtatgag tatgacagtt
      541 tatggtatga cagccaaaga ggtaacagca aaaacaccaa aaagctatgt cactggaacc
      601 aatagcgtat atggtccaaa gctgtcacaa gcccaactaa actcagtagc acaagcaaca
      661 gcagatttta tgaacaataa aattacaaag aacatgacta cagacgctaa gattttagtt
      721 gcctataatc acattaaaaa taacacaaca tatgttgact ggaatgcagt agaaggagcg
      781 aatactgcct atacattggt tactaaaaaa ggtgcatgtt caggaatggc taggagcatg
      841 aaagctttgt gtgacgctat gggaattgaa tcttattatg tacacagcac atcaaatgac
      901 caccagtgga atcttatacg ttttggtgat gggcgtttgt atcatgtaga tattgatgct
      961 aataaatcag caggaaagga tatcatatac aagagtctta gccacccatt accatttgat
     1021 aagacagcat atccaacagt tggagcagta gcacaaacgc aaaaaccagc actacctggg
     1081 aagcacacta tgttaccaca tcaagacggg gcaaaggttg ttggaaaatt tggagatata
     1141 aaggataaac atgtaatgta tgaaggtgtt agatgtgttg taattaattc atcagtgaat
     1201 acagttacat ttctaggtag taaatcaaat ttagacccat caaatcttac aattgctgta
     1261 ataggattta tgaacacacc atatgggatg tttgcattag caccagaagg tgaacctgtt
     1321 gaatatcctt ttaaatatga taagccattt tctttagatt ttagtggtaa aaatcgttct
     1381 ggtgcagaat tagcaaagta ctgtaaaaaa aatgatgtga ctattaaaat agaaatcact
     1441 gatgaaaaag ctaatattgg acttggtcaa ataatgtata ttcgatatga ataatgattt
     1501 aatcatgaca gtagaataag ttataaacat cgcgccctct tactaggggc gcatttaata
     1561 ttatatctga tattttgtag tcaggtcaac tgctggattt ctcacctttt tatttataaa
     1621 aattattatc tctaagcatc tgagcatgtt tacgattaac taaaaattgt tttaactcag
     1681 tcaagcatag gaataaaata gctctgtttt caaactctgt tctgtcaaca ggtagaggat
     1741 ggtttttcat actaagcatt aatctcttca attcttcaag aagaagattg cctgtttcag
     1801 cttcaaattc agtataacct attttatgta tgaaagcaga tagaatgtga gcttgtggtg
     1861 gtgtagaatt tagttgagat acataactat acaaattttc taagatatcc cgttggcttt
     1921 ttataatatc catatgttct aagaagaatc tagtatcact taataaagta ttattaatat
     1981 tatcgtaagt ttcagaaata ctgctgtcaa taagtgaatt taatttatat aaatctctac
     2041 tatatccatt ttctttttta ggatttacaa taatatctgc aatgtctata agtacaatac
     2101 tcacttcttc ctgtaacttt ttttgaactt catggatttt atgtatattt gttggaatat
     2161 acatatttat taagacacct attccagttc ctactgcaaa tattccagct tcatttaaaa
     2221 tccatttgat agaagtttct ccttgaaggt agtaatggga tgctatgact acacacatag
     2281 caataacatc ttgaatattt aacaaaaaac aaagtggtat aattataaat ataaatattg
     2341 aaaatgaagt caaattatat cctaaaatat taaaacataa atatgaaaaa atagtacata
     2401 aaagaaaacc ataaatcttt ccaatggagc ctttaagagt ttctctttta gtgtccttta
     2461 ctgtaagtag tactataact ccagcaacca ttgaatattc taattttaat agccaagcaa
     2521 tgaatatagc aagagaacat ccaagagata attttattac tttaagtgaa ttaatattat
     2581 tcaaatgcaa acctcctgta aatcaatata ataaaattct aacataaaac taatattgct
     2641 tgaaaggtaa gtaactatac ttaattattt tattgcccta tttgtttaga tacaatcaat
     2701 ataattttat tttatgaaaa ttttttgtaa aattttaaat ttagagacta acatgagacc
     2761 acaaagagga agtttattat aagaagaaaa cttaaaattg attttaaata gcaaatgaaa
     2821 ataatcaaat atgtatatac tattattaaa aaataaaatt atatagttaa tataatttta
     2881 taattggttt agtatagaga tttttaactt ttatgttata ctatttataa aagtaggtat
     2941 aaagattaaa agacataatt aaatacgtat gagaggtttt aaatagtatt gttaatgaac
     3001 ttgattattg tttataatta caaattggag gagataaata tgaaaattac tgaaggatat
     3061 atgcctttta aaggttttaa aacgtattac cgtatagtag gagaaaatac ggaagggaag
     3121 aagccattag tattacttca tggaggtcca ggttctacac acaactattt tgaggtattg
     3181 gataagatag ctgaaagtgg tagacaagta ataatgtatg accaaattgg ttgtggaaat
     3241 tcctttgtag aagggcatcc tgagttattt aatgcagata cttggataga ggagcttatt
     3301 gaactaagaa aacatctagg acttgatgaa attcatctgt taggtcaatc ttggggaggt
     3361 atgcaggcta tttggtatgc tattgaatat aaaccaaaag gtattaagtc ttacatcctt
     3421 tcatctactc tttcttctgc aaaactttgg gagaaagaac aaaaaagaag aatatcatat
     3481 atgagtgagg ttgaccaaaa agcattgctt gatgcagtaa atactggaga ttactctaat
     3541 aaagaatata atgatgcatt agaaagattt atggaaatgt attgtgcagg tgaagtgaca
     3601 gaagattctc cagagtgcct gagaagacct aaaaaatcag gttctgaagc atatattgta
     3661 ggatggggac aaaatgaatt ttctcctaca ggaactcttt ctggatatga atttacagat
     3721 agattacatg aaataaaaga gccttgtcta gtaactagtg gagcaataga tttatgttcg
     3781 ccatatattg caaagactat gtatgataga atacctaata gtaagtggga gttatttgag
     3841 tattcaagac atatgccatt cgttgaagaa aatgagaaat atataaaagt cttaactgag
     3901 tggttgaatg cgaacgatta aaaactatat aaatttatat aaagaaatga gtgtctcaaa
     3961 atgaactttt tagttcatta ggcactcttt ttgcatgaaa tcaaatatat tttcattatt
     4021 tgaacgataa aatgaggctt atatctat
//
LOCUS       DAELSY010000234         4042 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.236, whole genome shotgun sequence.
ACCESSION   DAELSY010000234 DAELSY010000000
VERSION     DAELSY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.236"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            165..1796
                     /locus_tag="KPG09_003654"
     CDS             165..1796
                     /locus_tag="KPG09_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0968210.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(1931..2857)
                     /gene="fba"
                     /locus_tag="KPG09_003655"
     CDS             complement(1931..2857)
                     /gene="fba"
                     /locus_tag="KPG09_003655"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBG0968211.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            3187..3516
                     /locus_tag="KPG09_003656"
     CDS             3187..3516
                     /locus_tag="KPG09_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBG0968212.1"
                     /translation="MPQIKIRGINENDICKISKKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            3516..3719
                     /locus_tag="KPG09_003657"
                     /pseudo
     CDS             3516..3719
                     /locus_tag="KPG09_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
BASE COUNT         1606 a          504 c          696 g         1236 t
ORIGIN      
        1 ataatcatta aaattatatc ttagttacgt tttaatgaaa taaactactt gaaaagacga
       61 atattataag gagttgatta tatgtgctga tggtatttat tgtaagcaaa caataaattt
      121 tgtatgtagt tcaataaatt acttaatgaa aggtgcgaat attaatggat aataatttaa
      181 tagggcttat agaacatgta gaaaatccag ccattttatg taaagaatca ggcgaaataa
      241 tatactgtaa tcatctaata gatagtattt ttagttttct agatataaaa aagcctagaa
      301 atataaatga attagattca aattttgaca aaacagaaat actgacagat agtaaaaaga
      361 aaatagcttt cagagagtta aggatgactg cacatatata caatatgaaa gataataata
      421 atgaaaataa tatagtttat ttatttgaga agtctctaat atctgataaa gtaattgaag
      481 atataataga gcatatagat gaagttgttg ttgtgtttaa caaagatggg gtaattgaaa
      541 agatgaacac tgtcagtgat gagatattac cttttaaaag aactgaggta cttggtagaa
      601 atataacaga ccttgtgaga caaggattag ttgaagaacc aataatatta aacatgctta
      661 aagtgaagaa aaagatttat agaaatatcg tttacccaga tgggaagctt attgcatata
      721 cggcagttcc aagatgggat tctaagggaa agcttacagg aggagtacta acagggagag
      781 atatttcaag ggttattaag cttgaatctc agataaaata tagtgatata tcagaagaca
      841 cagagtatat aagtcaaagt aagattatgg acaatataaa aaaagttgtt aagagagctg
      901 cagcctcaga ttcttctata tttataaatg gagaatcagg tgtaggaaaa gaaataatag
      961 caagaacaat atataagtat agttcaagga gagacaaacc ttttatagct ataaactgtg
     1021 gagctatacc aaatgaatta ttagagtcag agttttttgg atatgaagaa ggttctttta
     1081 ctggagctaa gaaaaaaggt aaaaagggac tttttgaaga ggcgaatggt ggaactattt
     1141 tcttggatga gataggtgag ttaccaatgc agatgcaaaa aaaattactt agggttatac
     1201 aagaaaatac tattactaga ataggtggta gtaagcctat aaaaatagat gttagatata
     1261 taagtgctac taatatttct catgaagacc ttagaaataa ccttaaattt agacaagatt
     1321 tgtactatag attaagtgtt attccagtaa aaatacctcc tcttagagaa agaaaagagg
     1381 atatagtacc attagtaaat tatttcttaa aactttacaa tgaaaagtac aatagagaag
     1441 tagaagtttc acctaaagtt attgagttat tagaagaata ttcttggcca ggaaacataa
     1501 gagaattaaa aaacatcatt gaaagatttg tagtattatc agctaaaaat gtaattggag
     1561 aagatgaatt taacatgctg ataaatttag atatgattga taatgaaaca gatgatttat
     1621 caccaatagt ggtaaatgga attatgaatc ttaatgatgc atataaaata gtggaccaaa
     1681 taatgataag taaggctata aataagtatg gttcaataac aaaggctgct gaagttatag
     1741 gtatatatcc ttctaccatc catagaaaga taaagagtgg acatattcac gtttagctgt
     1801 cagtttaaaa agacaatata attaaaatat tataagatac ataattgata caacttaaaa
     1861 gagatggctt aaaaataaat ttaattactt taagtcatct cttttctttt ataaatattt
     1921 aaaagttata ttagtttata gagttagcag aacctaaaac atcatctatc ttgctttcaa
     1981 ctacagcttg tatagcatct ctaccagcac caatgtattt tcttgggtca aattcttttg
     2041 gattttcagc taagaatttt ctgatagcag cagtcatagc tagtcttaag tcagtatcca
     2101 tgtttatttt acaaacagcc atagaagatg cttttcttaa catatctaca ggtacacctt
     2161 tagcaccagc tatatttcca ccaaattcat tacatgtagc aacagcgttt tggtcaacag
     2221 cagatgcacc atgaagaact attgggaaac ctggtaattt gctttggatt tcttctagta
     2281 tatcaaatct taatttagct tctcctttga atttgaaagc accatgagaa gttccaatag
     2341 ctattgctaa tgaatcaaca cctgttcttt caacgaactc aactgcctca gctggttgag
     2401 tatacttgtg aacatcagaa gtaacatcat cttctgttcc agctagaaca ccaagttctg
     2461 cttcaacaac aacaccttta gagtgtgcat attcaacagc ttctttagtt attctaacat
     2521 tttcttcaaa gtcaaaatga gaaccatcta tcataactga agagaaacca gcatcaatac
     2581 aagttttaat agcatccata tttggaccat ggtctaagtg aagagctaca tctacaccta
     2641 tttcatcaga agcagcctta accatttcaa ctaatgtatg aggtccagca tatttaacag
     2701 ctgacataga agcttgtatc ataacataag aatttttagc tttagcagct tttaaaacac
     2761 cttgtaattg ttctaagtca cttatgttaa atgcacctat agcaaatcca ccttcgtaag
     2821 cttttttaaa catttctttt gtagtaatta atgccatttt aaactcctcc tattaacttt
     2881 taaaatttac ccatcactta tattataacg ttaaatcaaa aaaagtatat agtaaattgt
     2941 aataactata aattacaagt ataatgttaa atagaagaca ataggattaa ataaaataat
     3001 aaagaatata aataatttag gaaaattagc tatataatgc taactataag ggtaggaact
     3061 accttattag tttggagata ctgataacaa tagttgtctt gaccaagaag ttaccacttc
     3121 aaacgttatc gaagggaatt aagtggatga taattcacta aaaatttaaa ataaaggaga
     3181 taaactatgc cacagataaa aataagagga attaatgaaa atgatatatg taaaataagt
     3241 aaaaaaatga taaatgattt agttgaagct gtaaaatgtc caagagacta ctttgaaata
     3301 gaatgtataa agtcagttgc aataagagat ggtaaaatag cagatgtata tccttttgtt
     3361 gaagtagctt ggtttgatag aggacaagaa gtacaagata tagtagcaag aataataact
     3421 gatagtataa gaaataattt agatgtagaa agtatggatt tagcatttac agtatttgaa
     3481 aaagaaaaat attatgagaa tggagagcat ttttaatggc taaaaagcaa cgtatagata
     3541 aaattctttc aaatttaggt tatggaagta gaagtgaaat aaagaagtat tgtaaacaag
     3601 ggtctgtagt agtaaatgga agtgaagttt caaatccagg aactcaagta gacactgaaa
     3661 atgatgagat attatttaat ggagaagaag ttatatatag agaatatata tatttgatta
     3721 aaaaatagct acgtacactc gtgactttag tcatgagtta gtagctaaaa tagtcagcat
     3781 atagggaaac ttatatgtag agataggata gagcctatcc aatactactt gaattgctgg
     3841 aaacccctaa agctaaccaa actaaaacat aaggatgaaa taaacctaag tgtgaaagtt
     3901 acgaaagtag aaaaaattgg ttagatggta taaggttaaa ccctaagtac tttaaaatgg
     3961 gcaatcagca ggtaagctcc gaataggaga aacttcaacg actattcctc ttgagggaag
     4021 tacactacaa gctatcggta gt
//
LOCUS       DAELSY010000235         3939 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.237, whole genome shotgun sequence.
ACCESSION   DAELSY010000235 DAELSY010000000
VERSION     DAELSY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.237"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..239
                     /locus_tag="KPG09_003658"
     CDS             <1..239
                     /locus_tag="KPG09_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005209661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968213.1"
                     /translation="VEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRTAIEF
                     STEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            497..1546
                     /gene="carA"
                     /locus_tag="KPG09_003659"
     CDS             497..1546
                     /gene="carA"
                     /locus_tag="KPG09_003659"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG0968214.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            1739..>3939
                     /gene="carB"
                     /locus_tag="KPG09_003660"
     CDS             1739..>3939
                     /gene="carB"
                     /locus_tag="KPG09_003660"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HBG0968215.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYL"
BASE COUNT         1630 a          400 c          777 g         1132 t
ORIGIN      
        1 gagtagaaga atctagacca aacatattag atgctataag aaataaacaa gttgatatag
       61 taataaatac accaactaaa ggaaatgact ctacaagaga tggatttaag ataagaagaa
      121 cagctattga attttctact gaaataatga catctttgga tacactaaaa gctttagtag
      181 aagttaagaa gaaacattta aataaagatg agttaaaagt ttacaatata gctgaataga
      241 aattaagttg attgtatagt ttaagaattt aattattaaa taaaaaaaaa gaggttattt
      301 tttctaggag tttttacttt tgagataact tctttttttt aagaaatttg tagtataata
      361 gaaaaagata aaaaataaat gtaaataagt ataaaactag aatgtaaaag tgaaaaatag
      421 atatagatat ttaaaagctt atatttagtt aatgttcttt agtttgtcta aagagaatta
      481 taggaggtac tgtagtatga aagcaaggtt aatattagaa gatggaacag tttttatagg
      541 aaaggcattt ggatatttag aagaaagtgt aggagaagta gtatttaata cttcgatgat
      601 aggatatggt gaagttttaa cagacccttc ttactatggt caaatagtta ctatgactta
      661 tcctcttgta ggaaattatg gtataaattt atcatcagca gagtctgaaa aagttcaagt
      721 aaagggattc atagtaaggg agaaaagtga ttctccaagt aattttagat gtgaaattga
      781 tatagaccaa tatttaaagc aaaataaagt tatcggactt gaaggaatag atacaagagc
      841 tttgactaag atacttagaa ataatggaac aatgaaaggt ataattacat tagaagacag
      901 taaattagaa gatgttaaac ataaattgga taagttttca aatacagaag cagttagaac
      961 tgttacaaga aaagaagtag agcatataaa aggaaatggt ccaaaagtgg ctgttatgga
     1021 ttttggagta aaaagaaata tattaagatc atttatagct cgtggatgtg atataacaat
     1081 atttccagct acaacatctc cagaagatgt attgagtata aaccctgact taatattctt
     1141 gtcaaatgga cctggagacc cagaagacct agaagatgtt atagaaaata taaaagctct
     1201 tattggtaaa aaacctatag ttggaatatg cttaggacat caacttttag ctttagctct
     1261 tggaggaaaa actgctaaac ttaaatttgg gcatagaggt ggaaatcatc cagttaaaga
     1321 tttagaagaa ggaaaagtat ttataacttc tcaaaatcat ggttattatg tttcagaagt
     1381 tcctgaacaa atgaaagtga cacatataaa tttaaatgat aatactgttg aaggaatgag
     1441 acatgagaag ctagatgtat acagtgttca ataccatcct gaagcttgcc caggaccaaa
     1501 agataatgac tatatttttg ataaattttt agaattagta aaataaataa cattagaata
     1561 gctaactaaa aaacaagaat aacttaaatc aaaaagtttg gtttaattaa catagatgta
     1621 ttaaatagat gtattgatga gtaaatttga acttaagata aaataaagta attaaagttg
     1681 attgtataga tgatgaagat aagaactgtg aaaattgttt agataaggag aaagagttat
     1741 gcctaaatta gatagtataa agaaaacttt agtattaggg tcagggccaa taataatagg
     1801 tcaagctgca gagtttgact attcaggaac acaggcctgc caagcattaa aagaagaggg
     1861 aattgaagtt gtattagtaa atagtaaccc agctactata atgactgata aagaaattgc
     1921 agataaaata tatatagaac cattaacaat agaatttata gaaaaaataa tagaaaaaga
     1981 aagaccagac agtttacttg caggtatggg tggtcagaca ggattaaact tagctgtaga
     2041 attacatgat gctggtatat tagataaata taatgtaaaa gttataggaa catctataga
     2101 atctataaaa aagggcgaag atagagattt atttagagaa gtaatgaaag aaataaatca
     2161 accagtaata gttagtgata tagttacaaa tttagaagca ggtcttgagt ttgccaataa
     2221 gataggatat ccagtagttg taagaccagc atatacgcta ggtggaactg gtggaggtat
     2281 agctgatact gaggaagaat taagagaaat actttcacat ggactacagc taagtcctgt
     2341 tggacaagtt cttcttgaaa agagtataaa aggttggaaa gaaatagaat atgaagtaat
     2401 gagagatgga aatggaaact gtataactgt atgtaatatg gaaaatgtag acccagttgg
     2461 agttcataca ggagacagta tagttgttgc tccaagtcaa actctaagtg atgaagagta
     2521 tcaactactt agaaaggcat caatagacat aataaatgca atagaggttc aaggtggatg
     2581 taacgttcaa atagctttaa acccacatag tttagaatat gcgataatag aaataaaccc
     2641 aagagttagt aggtcatcag ccctagcatc aaaagctaca ggatacccaa tagcaaaagt
     2701 agcagctaag atagctttag gttatacttt ggatgagata gaaaatgcag taactaaaaa
     2761 aacttatgct tgttttgagc caacattaga ttatgtggta gtaaaaatac caaagtggcc
     2821 atttgataaa tttagacatg caaatagaaa attaggaact aagatgatgg caactggtga
     2881 aatcatgagt ataggaagta actttgaggc tgctattctt aaagggataa gatcacttga
     2941 aacaggaaaa tattcattgg ttcatgctcc atcagaaggt agaacattag aagaattaaa
     3001 ggctagagta gtagtacctg atgatgagag actatttgac ttagctgaaa tgataagaag
     3061 aggctataaa gtagaaatga tatcagaaat aactggtgtt gataagtggt ttataaataa
     3121 atttaaatgg atagtagaac aagaagaaaa gttaaaagtt ctaaaaatag aagacttaga
     3181 taaagaatat ttacatgaat taaagaaaaa gggattttct gataaaggta tatctgactt
     3241 aatgaagata agcccagaaa aattatatga attgagaagt ttatataata tacaaccagt
     3301 ttataaaatg gttgatacat gtggaggaga atttgaagct ctatcacctt attactattc
     3361 aacttatgaa caatatgatg aggtagtagt tagtgataag agaaaagttg tagtattggg
     3421 ttctggtcca ataagaatag gtcaaggtat agaatttgac tattgttcag ttcactgtgt
     3481 aaaatcttta agaaaaatgg gaatagaaac tataattgta aacaataacc ctgaaactgt
     3541 aagtacggat tttgatacat ctgataagtt gtattttgaa ccattaacag aagaagaagt
     3601 tttaaatata atagaaaaag aaaaaccaga aggtgtaata ctacagtttg gtggacaaac
     3661 agctataaaa ttggctaagt ttttacatga aaaaaatata cctatactgg gaacagactt
     3721 tagagatata gatgcagcag aagatagaga aaaatttgat gatttactag agagactaga
     3781 tataaataga ccaaaaggaa aaggtgtatg gacaactaat gaaggtgttg aaattgctaa
     3841 ggagttaggt tatccagtat tagttagacc atcttatgta cttggtggtc aaggtatgga
     3901 aataacttat aacgaagaaa aactaattca gtatttgga
//
LOCUS       DAELSY010000236         3934 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.238, whole genome shotgun sequence.
ACCESSION   DAELSY010000236 DAELSY010000000
VERSION     DAELSY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.238"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            2..652
                     /locus_tag="KPG09_003661"
     CDS             2..652
                     /locus_tag="KPG09_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG0968216.1"
                     /translation="MKYFLELFPIIMKSFNVTVILAISSLVFSLIIGTIVALSSYYKV
                     KVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMNDFVKNMDPVFATSIVLSLNMGAYM
                     SETIRASILSIDKGQIEAAYVMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLIKGSS
                     VAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKISNY"
     gene            712..1509
                     /locus_tag="KPG09_003662"
     CDS             712..1509
                     /locus_tag="KPG09_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0968217.1"
                     /translation="MVKRNIIKCLVAVLSLTFVVAGCSKKEEVKDEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVAASVNKD
                     NLFEEKVYGDGTNGLQDLDLGRVSAWIDAELTVVGDAKKANMKVRVLDEKLSVTNIAY
                     FFKKNDEKSKTIKEDVNKAIEEMLSDGTVKKISEKWFGMDVSADIQK"
     gene            1642..2817
                     /locus_tag="KPG09_003663"
     CDS             1642..2817
                     /locus_tag="KPG09_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0968218.1"
                     /translation="MIETKIKSIALKNREKIIKIRRQIHSNPELAFKEFKTSKLIKEE
                     LNKLKIEYIDVAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSINDNMHA
                     CGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGVDALAT
                     GHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAVYSSIQ
                     QIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKILENIV
                     RNVCRAYGADCKFEKSTGGDAVINDKNMIELGRESACKILGVENVEIIDFPAMTGEDF
                     AIYMKEKPGLFMYIGVGNKEKNINYRLHSNKFNIDEKCLSIASSLFSQLAVDYLCQL"
     gene            2926..3675
                     /locus_tag="KPG09_003664"
     CDS             2926..3675
                     /locus_tag="KPG09_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG0968219.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEINQSEVVAILGPSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDIANLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSNKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIVRKEEVFS"
BASE COUNT         1572 a          392 c          743 g         1227 t
ORIGIN      
        1 tatgaaatat tttttagaat tatttccaat aataatgaaa tcgttcaatg taactgtaat
       61 tttggcaata agttcattag tattttcatt aattataggt acaatagtag cattatcatc
      121 ttattacaaa gtgaaagtat tgaatcaaat atgtaaagta tatgtatcta tctttagagg
      181 tacgcctctt atgccacagt tattcttttt atattttgga ctagcgtata tgaacgattt
      241 tgttaaaaat atggacccag tatttgcaac atctatagta cttagtttaa atatgggagc
      301 ttatatgtcc gaaacgataa gggcatctat actttcaatt gataaaggtc agatagaagc
      361 agcatatgta atgggattaa caaatttaca aacaatgaaa aggatagtga taccacaggc
      421 cgtaagagtt gcactgccat cattattcaa taattttata gatttaatta aagggtcttc
      481 tgtagctttt gttgttggag taaatgatat aatgggtgct gccaaatcac aaggagcgtt
      541 gagttttaag ttctttgaag tgtatggagc agttatggta atttactggt gcataataac
      601 tcctttaggg tgggggcaaa aaaaattaga aaagaaaata agcaattatt aagttattaa
      661 gaaaatacat aaaagtattt ttatataaaa aatggtgtga ggagaagaga tatggtaaaa
      721 agaaatatta taaaatgttt agtggcagtt ttatcgttaa catttgtagt tgctggttgt
      781 tcaaaaaaag aggaagtaaa ggatgaaaaa gttataactg tagcttcatc taattcagct
      841 gacccatatt cttatgttga taatggtgag cataagggtt ttgaagttga tatgtggaaa
      901 gagattggta aaagaagtgg atataagata aaaatggaac caacaggttt tagttctata
      961 tttgggatgt tagattctgg taaagttgat gtggcagcca atttctttgg aatgtctaaa
     1021 gaaagattag aaaaatacga tgcttctata ccttatggtt ctgattctgt atgtatagct
     1081 gtaaaagatg gaaacaacga aattaataaa cttgaagatt taaaaggaaa agttgtagca
     1141 gtttcagaag gttctcaagg gcaacaggtt gcagctagtg ttaataaaga taatttgttt
     1201 gaagaaaagg tttatggtga tggaactaat ggtcttcaag atttagattt aggaagagtt
     1261 agcgcatgga ttgatgcaga acttactgtt gtaggagacg ctaaaaaagc taatatgaag
     1321 gtaagagtat tagatgaaaa attatcagtt actaatatag catacttctt taagaaaaat
     1381 gatgaaaaat ctaaaactat aaaagaagat gtaaataaag caatagaaga aatgctttca
     1441 gatggaacag tgaaaaaaat atctgaaaaa tggtttggaa tggatgtaag tgctgatata
     1501 caaaaataaa tactttaaaa ttaaaatata aaactgtgat aactatagat aagtatacta
     1561 aatttatcta tagttatctg tgtattacaa tgtatttaga ttagtcttta taaattaaaa
     1621 tgagttatgg aggtcttgca aatgattgaa actaaaataa aatccattgc actaaaaaat
     1681 agagaaaaaa tcataaaaat aagacgacag atacacagta atccagagtt agcatttaaa
     1741 gaatttaaaa catccaaact tataaaagaa gagttaaata aacttaaaat agaatacata
     1801 gatgttgctg gtacaggagt attagctact ataaaaggaa aaaataatgg tgggaaaact
     1861 atattgctta gagcggatat ggatgccctt ccaatcaaag aggaaaatga tttagaattt
     1921 aaatcaataa atgataatat gcatgcttgt gggcatgatg cccatgtttc atggttgtta
     1981 ggaacagcaa tgattttaaa tgacataaaa gaagaattga atggaaatgt aaaattacta
     2041 tttcaacctg gcgaagaaaa aggtggttct gatattgtaa ttaaagaaaa tgtacttgaa
     2101 ggtgtggatg cattagcaac tggtcattgt tggccaacta ttgaatcagg aaaaattgga
     2161 atcgctagaa attgtgctat ggcagcgact aatacttttg aaataacaat aattggcaag
     2221 ggcgggcatg gagcagagcc tcataattgt atagacccaa ttgcagttgg aaatgcagta
     2281 tattcatcca ttcaacagat agtttcaaga aaaattgacc cagttgtacc tgtagtagtg
     2341 tctgtttgtt cttttaattc tggtgtttct aaaaatataa taccagatgt atgcacactt
     2401 caagggacta ttagagcgat aagcaaggaa caagtaattg aagtaagtaa aattctagaa
     2461 aatatagtaa gaaatgtatg tagagcatac ggagcagatt gcaaatttga aaagagtaca
     2521 ggaggagatg ctgttataaa tgacaaaaac atgatagagc taggaagaga aagtgcttgc
     2581 aaaatacttg gagttgaaaa tgttgaaata attgattttc ctgcaatgac aggtgaggat
     2641 tttgctattt atatgaaaga aaaacctggt ttatttatgt atataggtgt aggaaataaa
     2701 gaaaaaaata taaactacag attgcatagt aacaagttta atatagatga aaaatgtctt
     2761 agtatagcat cttcattatt ctcacaactt gcagtagatt atttatgtca actatagagt
     2821 acattcataa aaatatattt agcctatttt taaatttaat gctagtatat taattttgat
     2881 gtagaacata ttatttatta tatttattta aaaaggtgga taacaatgat aaaagtagaa
     2941 aatttacata aaagttttaa agggacagaa gttcttaaag gtataaattt agagataaat
     3001 caaagtgaag tagtagctat acttggacca tctggaacag gtaaatctac gttacttaga
     3061 tgtttaaatt atttagttga gcctacaaaa ggaaagataa tgattggtga tgtaaaagta
     3121 gatgtagaaa aagcaacaaa aaaagatata gccaatttaa gaaaacatac atctatggta
     3181 tttcaaaatt ataacttatt taaaaataaa acagcattag aaaatgtaat ggaggcattg
     3241 atagtagtac ataaaaagag taataaagaa gctagagaaa tatctttaaa attaatagat
     3301 aaagtaggta tgttggatag gaaagatttt tatccatcaa aattgtctgg ggggcagcaa
     3361 caaaggatag gtatagcaag agcgatggca gtaaatccaa atgtaatatt atttgatgag
     3421 ccaacatctg cacttgaccc agaacttgtt ggagaagttt taagcgttat aaaatcgctt
     3481 gcgaaagaag gaataacaat gcttattgta actcacgaaa taaattttgc aaaagaagtt
     3541 gctgataaaa ttatatttat ggataatggt gtaatagctg aagaaggaac tcctgatgaa
     3601 atatttaata atcctaaaaa tgaaagaact gcaaaatttt taaatatcgt taggaaagaa
     3661 gaagtattta gttaaaagtg tataaattaa gtattagaag atatgtaaga tactcccatt
     3721 aaatttataa gttagataat aaatttaatg gaagtatctt tttattatta gatttattat
     3781 ttaatttaaa attagaaatg taactatttg aaaattcttt ttaaaagtat atcaaaaata
     3841 tcaagtacat gaaaaaataa tatttgacaa aatatcatat atgtttacaa aattaacttg
     3901 atggaatgat aaagcatagg aattacaatg agta
//
LOCUS       DAELSY010000237         3852 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.239, whole genome shotgun sequence.
ACCESSION   DAELSY010000237 DAELSY010000000
VERSION     DAELSY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.239"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            61..1173
                     /locus_tag="KPG09_003665"
     CDS             61..1173
                     /locus_tag="KPG09_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0968220.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(1381..1857)
                     /locus_tag="KPG09_003666"
     CDS             complement(1381..1857)
                     /locus_tag="KPG09_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968221.1"
                     /translation="MVKKRLVAVVLSLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQPNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2056..3681)
                     /locus_tag="KPG09_003667"
     CDS             complement(2056..3681)
                     /locus_tag="KPG09_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0968222.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
BASE COUNT         1347 a          612 c          425 g         1468 t
ORIGIN      
        1 ttattttgtg atatatatat gttataataa atgtataaaa ttttgattgg tgggttatat
       61 atgaatgcat ttattagaaa aagaaataaa aactatgttg tttacctgga atttgaagat
      121 gaaaagacag gtaaaagaaa acaaaaaaat atgggtactt ttgataaaaa aagggaagca
      181 agtaaaagac taaatgaggt aaaagaaagc ttatataatg atggtttttt agtaccaagc
      241 gaaattacag tgtcagaatt tctattagat tttctaaata aatatagtga aaatatatct
      301 ttagctacat ataataatta tgttagtatt tgtaaaaatt acattaatcc atctattgga
      361 aaatataaaa tacaagactt acatccggtt cacatacaaa actacataga taaactatct
      421 tataaactaa atcctcaatc aattaagatt catataaata tattgaaact tgcaataaaa
      481 agagcttata gactcaaatt aataaaagaa aatgttatgg atagtataga ggctccaaga
      541 tataaaaaat ttaaaaatga gatttatgat agagaacaaa tgataaaatt attagattta
      601 gcaaaaaata cagaaatgga attacctatt aatttggcta ttggattagg tcttagaatt
      661 tcagaagttt taggtctaac ttgggataat attgattttg aagaaaatac aataacagta
      721 aataagataa cttcccgaat taatggttct gttattctca aagagccaaa gacagaaagc
      781 tctgttagaa aaatatcttc tcccaaagaa ttaatgtctt tattaaaaga atataaaata
      841 aaacagaata aaaatttatt aaaatcttcc attagaaata ataataattt gttgtttttc
      901 aataagaaat gtgaacctat tgctgaagat gttatgagta aaaaatttaa aagattttta
      961 gaaaagaatg aattacctca tattagattc catgatttaa gacactcaca tgttacttta
     1021 cttataaatt ctaaagtacc tataaaggtt atatccgaga gagtaggaca ctcaaatata
     1081 agtactactc taagcgttta ttctcatgta cttaaagaaa tggataaaga ggcttctgac
     1141 aaaatttctg aaagtttatt taatgctaat taatattaat ttatcttagc tttaaattag
     1201 cttagcttat tgagatagat tcattaatat taaatgtatc tatctcaaat taataactac
     1261 ttaaataatc tgttttattt taatttatcg cctcagttat tatacttttt tatgaaaaat
     1321 tatactttac aataaaaaag atatttcctt atagaaagta tctttttaaa tactatttta
     1381 ttatatactt gctaacattg gaatatctaa catcttcttc attacatcat ctactttttt
     1441 tgctttatat tcattgtatc caattaaata tcctttttca tatgaacttt ttaaaatctc
     1501 tttttcatat aaattatcag atttggcttc tgtgacactt aaattataaa gccctttaca
     1561 cccatcttca taacctttat tatacccttt ttctgcaaaa tagtttttat gatttttttc
     1621 gttatgtact gaagatattg ttactccaga attttctttt gtactagcat aaggacaaaa
     1681 acctttattt ggttgatgtg ctgaatgtcc tccacaataa taacgtgtta actctgattt
     1741 atttttatta tgatgacctc ctgatgaatt atgccctcct gagtgagcat atgaaaaatt
     1801 ccctacacta atcatagcta aactcaatac tacagctact aaccttttct taaccataat
     1861 ctattccccc caaaatgtta tatattttta ttatatcatc tactatatta tatttaaatt
     1921 cttaatatac aaaatattac tcgattctgc taataaacat atttaaacaa cataaaaaac
     1981 ctcctaatat ttgattaaaa atttacacac tcatgcatag catgtatcaa atattaaaag
     2041 gttttatact aataattatt tatcttttcc atacatttta gctaattggt cttgtatctc
     2101 tttactttct aaatactcat caaagtccat ttttctatcc attattccat ttggagttat
     2161 ctctataatt ctatttgcta tagtagatat aaattcatgg tcatgagaag taaataaaag
     2221 cactcctggg aacttttcaa gtcctttatt tacagaagtt atactttcta ggtctaagtg
     2281 gttagtaggg tcatctaata caagtacatt tgcatttgaa agcattagct tactaagcat
     2341 acatctaacc ttttctcctc cagatataac ttgagcttct tttaatgctt cttcacctga
     2401 gaataacatt ctacctaaaa atcctcttat aaaactttca cttttttctt cagagaactg
     2461 tctaagccag tcaaccaaag aatattctac tccgtcaaaa aacttattat gattttttgg
     2521 aagataatct tgagaagtag ttactcccca tttatactct ccactgtctg gttcaagttc
     2581 tcccataact atgttgaaaa gtgcagtagt agctatttca tctcccataa atactacttt
     2641 atcatctcta tccaatctaa atgacacatt atcaagaacc ttaaccccat ctatagtttt
     2701 agttaaattg tgtacttcta aaacttcatt cccaatttct cttgcaggtg taaatcctac
     2761 atatggatat cttcttcttg atggttgtat atcttcaact tctaatttct ctaactgttt
     2821 ctttcttgat gttgcttgtt tagcctttga agcattagaa ctaaatctag ctataaattc
     2881 ctttaattga gctatctttt cttcagtctt tttattttgg tccttagcca gttgtaaagc
     2941 caattgactt gactcatacc agaaatcata gtttccaacg tacatttgta ttttaccaaa
     3001 atctacgtca actatattag tacatatttg atttaggaaa tgtctatcat gtgatactat
     3061 tataactata gactcttcta aatccattat aaaattattt aaccagttta ttgatttaaa
     3121 atctaagtgg tttgtaggtt cgtccattaa tagtatttca ggcttaccaa aaagtgattg
     3181 tgctaacaat accttaacct tttccccacc aactagttcc ttcatttgtt tgtaatgcat
     3241 atctttcgtt atgccaagac ccataagtat tttctcagca ttagtttctg catcccatcc
     3301 gtcaagctct gcaaactcac cttcaagttc ggcagctttt atcccatctt cttcattaaa
     3361 gtcttccttc atatacaatg catctttttc tttcattatg ttccaaagtc tctcatgacc
     3421 tcttataaca acatttaaaa cagtttcttc ttcatattca aagtggtcct gttttaaaac
     3481 tgacattctt tctttatcag ttatagatat actacctgta ttaggctcta tttctcctga
     3541 taatattttt aaaaaagtag attttcccgc accattagct cctattattc catagcaatt
     3601 acctttagtg aattttaagt taacatcttt aaatagttcc ttatcaccaa atctaagtcc
     3661 aacacctgta acttgtaaca ttaatatatt ttcctttctt tttgctcata atttatttta
     3721 tcataaaact ttgataacat ttttattata tattatcacg taaatagtat attttttata
     3781 ctaaatttgt gaaatattta ttaaaataat actaaaaatt gtaaatttat aaaaattaac
     3841 aatctcttat at
//
LOCUS       DAELSY010000238         3852 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.240, whole genome shotgun sequence.
ACCESSION   DAELSY010000238 DAELSY010000000
VERSION     DAELSY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.240"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            266..2617
                     /locus_tag="KPG09_003668"
     CDS             266..2617
                     /locus_tag="KPG09_003668"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBG0968223.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERFEVQGNKKMKNFPFLMGQGVWKGSDDLVPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            2639..3178
                     /gene="nrdG"
                     /locus_tag="KPG09_003669"
     CDS             2639..3178
                     /gene="nrdG"
                     /locus_tag="KPG09_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBG0968224.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         1592 a          385 c          686 g         1189 t
ORIGIN      
        1 ttgcctaaaa ttagtgtaaa aaaaagagaa aaaaatttta actttcaaag ttgttagaaa
       61 cgctactgtt caaaatatcc acatagtatt tgcaatgatt tgcgcaagtt tgactattat
      121 gtacaaattt ggtaatctta atatagcaat tattaagata atccctttaa cttagttgaa
      181 ggggattttt ttagacttaa catactacat atagtggtat tgggattaat aaaatacaat
      241 atatagtaac aggaggtttt tttaattggt tgaattcgta aaaaaaagag atggaagagt
      301 aattccattt aatgaagata gaataacaag agctatattt ttagcagcaa cgaatgttgc
      361 agaaagagaa ggaatagtac ctgattataa attatctgag caattaactc aagaagtaat
      421 aaagttttta aatcataaat actcagaatc agttccaagt gttgaagata tacaagattc
      481 tgtagttaag gttcttatag aaactggcca tgcaaaaact agtgaagaat atataatata
      541 cagaactgaa agaagtagaa taagaaattc aaaaacaaga ttgatgaagg caatagagga
      601 aattaccttt gaagatgcag aagatgctga tataaaaaga gaaaatgcaa atattaatgg
      661 taatacagca atgggaacta tgttacaata tggaagcact gtatctaaag aattttgtaa
      721 aacacatata ttaaaaccag aacattcttt tgctcatgat aatggagata tacacataca
      781 tgatatggat tttttaaata tgggaacatt aacttgttgt caaatagatg ttaaaaaact
      841 attcaatggt ggattttcaa caggacatgg ctttttaaga gagccacaag atataataag
      901 ttatggtgca ttagctgcaa ttgctataca aagcaatcaa aacgatcaac atggaggtca
      961 aagtatacca tttttcgatt atggattggc tgaaggtgta tataagacat ttaagaaatt
     1021 ctacatagga aatttagcta aagcattgaa gttatttaaa ggaattgaaa ataatgatgt
     1081 gataaagaac atagtatata atacagagaa agaaacaaat caaaaagttg gtcttaagag
     1141 agatgaattg tatctaaact tagaaaaaga gaaattaata cagacttttg atatagatga
     1201 tgaacttgta aataagatgc aaaactttgc atttgaagaa tcgtatagag aaactgataa
     1261 aaaaacatat caatctatgg aagcatttat acacaatcta aatacaatgc actctagagc
     1321 aggtgcacag gtaccatttt caagtgtaaa cttcggaaca gatacttctg aagaaggaag
     1381 aatggtaact aagaatcttc tattatctca agaaagaggt ttaggtaatg gagagacacc
     1441 aatttttcca attttaatat ttaaggtcaa agaaggcata aatttaaatc ctgaagaccc
     1501 taattatgat ttatttaaat tatcatgcag agtatcagct aaaagactat ttcctaattt
     1561 tagtttttta gatgcgcctt ttaatgcaaa atattataaa aaaggtgaac cagatacaga
     1621 ggctacatat atgggttgta gaacaagagt acttagtaat gtgtgtggtt cagaaacagt
     1681 tagtggtaga ggaaacatat cttttactac agtaaattta ccaagattgg gtataaaaca
     1741 tggtattata aataatgaaa aggctaattt agatggtttc tttgaagaat tagatgagaa
     1801 gataaactta attatagaac aattattgga gcgctttgag gttcaaggaa ataaaaagat
     1861 gaagaatttt ccattcttaa tggggcaagg tgtatggaaa ggttctgatg atttagtacc
     1921 agaagataca ctaaaagaag taataaaaca aggaacattg acaattggat ttataggttt
     1981 agcagagtgc cttattgctc ttataggtaa acatcatgga gaaagtaaag aagctcaaga
     2041 attaggatta aaaatagtgt cacatatgag acataaaatg gatgaagcaa cggacaaata
     2101 taaattaaat ttctccttaa tgggtactcc agcagaaggt ctttcaggta ggtttactaa
     2161 aatagataaa aaagtttatg gagaaattaa aggtatcaca gataaagaat attatactaa
     2221 ttcattccat gtaccagttt attacaatat aagtgcatat gataaaattg agatagaagc
     2281 tccataccat gaattgacta atgcaggtca tataacatat gtagagttag atggagatcc
     2341 atctgataat ttagaggctt ttgaaactgt tataaaagct atgaaagatt tgggtatagg
     2401 atatggaagt ataaatcatc ctgtagatag agacccaata tgcggttttt caggtgtaat
     2461 aacaagtaat atatgccctg tatgtggaag aaatgaagat gaaagtgaca tcaaattcga
     2521 aagaataaga agaataacag gatacttagt tggtacagtt gatagattta ataatgctaa
     2581 aaaagcagaa gtaagagata gagtaaaaca tagatagttt tatttggggg aataagttat
     2641 gaaaataaga atgtcatcta ctataagtta tgacagcata gttgatggac caggattaag
     2701 aatggtaata tggactcaag gatgtattca taattgtaaa gagtgccata atcctcagac
     2761 acatgattta tgtggaggat tttatatgga tacagaagaa attataaata aagtcaaatc
     2821 attgaaatta caaaaaggca ttacgctatc aggaggagaa ccatttttac aaccagaacc
     2881 attagaagaa atagcaagag aagctaaaat aaatgggtta gatgtatggt catatactgg
     2941 atttacgttt gaacaactgt tagataaaaa aaatagagcg tattttaaaa atttaaattt
     3001 actaaagcaa attgatattt tagtggatgg aaaatttata gctgaaaaaa aagatataag
     3061 cttaaagttt agagggtcat caaatcaaag gataattgat gttcaaaaaa gtttgaaata
     3121 taaaaaagtg tttttggttg agcaatatat gaaggatgat ttatctatag cagaataaaa
     3181 tataaataaa caggattaaa tatatgcata tatttaatcc tgtttatttt catataatat
     3241 ttcaagtttg tataatgtaa caattaaacc aaaagactca ttgtatattt gaaattaagt
     3301 tttttatagt ttataggtgt tttatgtgga ttacgaatga tatagtatcg atacattatt
     3361 taatcgattc ttattccaag aaacaattaa tttatagaaa ttcttaattg ttacaatata
     3421 aatatctaga taagttataa gatactgata aataatttat aaagtttatt aatctaatat
     3481 tgtgtgtgaa tgttaagtgg tcaagtagtc taaaaaatga aaaagattta aaaaagtgtt
     3541 ttaactaaac acataagtgt taaattcatg tataagtata ggttagatac ataagaaata
     3601 attatgtaat atctatcttt gtaaaaaaat attaaatttt gtaatttgtg gataaatatg
     3661 tttataatgt ggataatatt taatgtaata agttaaagca atatgaatca actgtttata
     3721 atgtggataa tattataaat gtatgaataa ttgcagtatt taaaaacaaa aaaagttaaa
     3781 aaagttgttg actctaaaag aaaaagttgg tatagtaata cttgtcctca agaaaagggc
     3841 aaacaaataa ag
//
LOCUS       DAELSY010000239         3823 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.241, whole genome shotgun sequence.
ACCESSION   DAELSY010000239 DAELSY010000000
VERSION     DAELSY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.241"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            127..804
                     /locus_tag="KPG09_003670"
     CDS             127..804
                     /locus_tag="KPG09_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0968225.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVERI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     gene            876..2891
                     /locus_tag="KPG09_003671"
     CDS             876..2891
                     /locus_tag="KPG09_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG0968226.1"
                     /translation="MKKYNILKGTLIVLIVSLLAIAGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGEDKLLSLELPEIFSASEVWINGEIVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKDTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKLYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDSSY
                     FMVVLCIICINGSISKIEEDKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFIIVILFSLMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KKFLADVAHDLKAPVSAIQSFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNDFIKEIYENNLPDTQACGVNFETYILEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKVSDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            2941..3621
                     /locus_tag="KPG09_003672"
     CDS             2941..3621
                     /locus_tag="KPG09_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0968227.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKEISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILTDGDGWTLCKKIRNVTTCPIVYMTYINEEQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
BASE COUNT         1510 a          378 c          652 g         1283 t
ORIGIN      
        1 atagtctagt ttgtaaagat attataaaat aataatacta gttatatatg aaaataaaac
       61 attataattt gtaataatta taaaacttga gattatgatt aaaatacctt tgaatggagt
      121 ggtgtaatgt ataatttact ggttgtagat gatgatgttg atattttaaa tataaatcaa
      181 atttattttt ctaatatagg ttttaatgta tatacatcag agactgctaa agatgctatg
      241 gatatagtag agcgtatagt tttagactgt ataattttgg atatttcatt gccagatata
      301 gatggatata aaatgtgcaa tgatataaaa gaaaaggtta acatacctat aatattttta
      361 tctaattatg agcatgaaga agaaaaagtt cggggatttt tagctggggg ggatgattat
      421 ataacaaaac cttatagttt aaaagaactt gaacttagaa tatatgctcg tatgaggcag
      481 tacaaaaata ttacatcagt tcctaatgtg ttaaagtttt cagcgttagt aataaatgta
      541 aattcaagaa aagtaacttt tgatggagaa agtatagatt taactactat ggaatttgaa
      601 atattattgt ttctagctga aaacaaagaa caagtatttt cgaagataga gatatataac
      661 caagtttgga aaatgcctga tattggagat caacatacag tgcaggttca tattgcacag
      721 atgagaaaaa aaattaattc tttaagcagg gagcatcaat acattcaaac cgtatgggga
      781 aaaggatata agtttgtacc ataagtgaga tactgtaatt aataaatagt tcttgtttta
      841 tttaatacta ctatgaaaga gatgtggttg atgcaatgaa aaagtataat atactaaaag
      901 gaactctcat tgtattgata gtctctctgt tggcaatagc aggattaaat cttctgtata
      961 aatatgataa taaatataca tataagtcta ttagagcaag taatggaatt ttaactatag
     1021 ataatgatac tttcactaaa ggtaaacttg tctttctgat agatgactgg gagttttata
     1081 atcataaaat atttaaaccg aatgatttta cagagcaaga tataaaacca gaatatgttt
     1141 ttataggtca atatccagat ttttctatga gaaaagggaa tcagtcacca tatgggaatg
     1201 ctacttatcg tatgagaata aaaaatgaag gtgaggataa gctactaagt ttagagcttc
     1261 ctgagatttt ttccgcaagt gaagtatgga taaatggaga aatagtttct aaattaggag
     1321 atgtaggaac agaaaaatat agacccaaaa ttaaaaattc agtagtaagt tttattgcaa
     1381 agaaagatac agaaattata atcaatgtat cgaatttttc acattattat agtggtttgt
     1441 attatccgcc tgcattagga gaaacaaagg acataagtaa tatgatgttt tatcgattac
     1501 ttttttatag tataatttgc ttcacaactt ttgcaatagc tatattttcg ttgtctgtat
     1561 ggtttctttc aaacaaaagt aaactttact tatattttgg atgtatgtgt gttttctttg
     1621 ccatccatgt atcctatccc tttgtacatt tgttaggact gcctttggta agctttacat
     1681 atgcggttga agattcgtcc tattttatgg tagttttatg tattatttgt ataaatggaa
     1741 gtatatccaa aatagaagaa gataaagtgt atagattttt aattttacca ttaggaatta
     1801 caatgagtat catatctata gtgataccaa ttattcttct tccatatgat atgtactttg
     1861 tcaacatata tgggaaatta atagacatat ataaatatat tgtatttata tatatattaa
     1921 tgtcttcgct ggtttcaata tataaaaaga catgctctga gcatataagt tcttatattt
     1981 tagtttcagt aaacactgtt tttggaatta gcatattatt tgatgcagtt actagtaata
     2041 gatttgagcc tatatttaca gggtggcaaa cagaatattg tggatttata attgttattt
     2101 tattttctct tatgatgata aacaggaata gaataataat aaaagagaat gaaaaactta
     2161 caaggaatct tgaacatgaa gttgaagtga gaactaatga acttacaaca ttgttaaatg
     2221 aacgtaaaaa attcttggca gatgtggcac atgatttaaa ggcacctgtt tcagctatac
     2281 aatcatttat agacttgata aaggttggta atattcatgt agatgaagag acaagaacct
     2341 atctttatgc tataaatcaa aagtcaaatg aagtacaaaa tagagtacgt agtttgcaag
     2401 aatttacatc acaagataaa agcattggta ttagtgaaaa aatatgtcta aatgatttta
     2461 ttaaagagat atatgaaaat aacttgccag atacacaagc atgtggtgta aactttgaaa
     2521 cttacatatt ggagaaaaaa atatatgcaa agataaataa agagatgctt acaagagcat
     2581 ttgagaattt aatttataat gctttgacat ttactccatt tgatggaaag ataattttat
     2641 caatggataa agaagatgga tttgcaatta ttaaagtttc tgataatgga aaaggtatac
     2701 ctccagaaaa tataccaaag gtatttgaca gattctttac agaaagagat aataatgagc
     2761 caaaagggca agggctaggg ttatatattg tcaaatccat tataagagag catggcggag
     2821 aaatctttgt agattcagaa ctaggaaaag gaactgtttt tacaataaaa ttaagtctat
     2881 gttttttata ataatagctt atttaatata aaagaataat gatatttggg agtgattata
     2941 atgcaaacta agatattgat aatagatgga gacaaggata attgccagaa gctaaaagga
     3001 tttctggagg aaaaagaaat aagtatagat ttagcatata attgtgaaga agctattgga
     3061 aaaatattta gcaataaata tgatttaata tttttagaaa taattctaac tgatggtgat
     3121 ggatggactc tttgtaaaaa aattagaaat gtaactactt gtccaattgt ttatatgact
     3181 tatataaatg aagaacaaag cattttaaat gctttaaatt ctggcggaga tgattactta
     3241 ataaagcctc taaatttaga aatattatat gcaaaagtaa aagctatttt aagaagaatg
     3301 aactcatatg taaataataa tgaaaataat acaaaattag aggaatataa tagaactagt
     3361 ggagtaataa agttagaaga caaaataata aaattgactc caacagaaaa taagctgtta
     3421 aactttttta tagagaatcc tgaaaagact ctgactacta aagagatata tgaacatgta
     3481 tggatgaatg agtacttaga ggataactat agtgtggtag ttgcagtgaa tggattaaga
     3541 aagaaaatag agaaagatta taaaaatcct cagaaaatta taacaataag aggagcagga
     3601 tattatttta ataaagaata ataaatatat taatctcatt aataattgtt ataaatgtga
     3661 aatgctaacg atatctaaac taaattatta taaaatagct gactgtaatt taattgataa
     3721 agcctattct tctagacttt gccagttaaa ttacagtcag ttttattata tgaaaaaatg
     3781 cgtaacagaa ctattatttt atagattata tttgaaaaga tag
//
LOCUS       DAELSY010000240         3818 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.242, whole genome shotgun sequence.
ACCESSION   DAELSY010000240 DAELSY010000000
VERSION     DAELSY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.242"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..304
                     /locus_tag="KPG09_003673"
     CDS             <1..304
                     /locus_tag="KPG09_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0968228.1"
                     /translation="LCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKNYVYSQLE
                     ALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKFDKNDY"
     gene            544..2277
                     /locus_tag="KPG09_003674"
     CDS             544..2277
                     /locus_tag="KPG09_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0968229.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDVFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   2603..3355
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2669..2697
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
BASE COUNT         1521 a          497 c          493 g         1307 t
ORIGIN      
        1 tttatgtgat tttgtttcac ttttaaataa agaattaata aattctactt ctattataag
       61 attaggagga gatgaattta ttgttatatt ctctagtgat gttgacaaaa attatgttta
      121 ttcacaacta gaagccttaa gagaaaattt tttaaaagta ttctctaaat ataagtatct
      181 ttcatttagt tatggtgttg atacagtaaa aagaaattta aaattgacaa tagttgaatt
      241 ggataaaaaa atgtataaaa acaaggagaa aaacaagaaa aaatttgata aaaatgatta
      301 ttaacattgt tattttaaaa taaattaaaa ttatctttct ttttttaaac ttgtacatta
      361 gtttataaat atgtatctat acaagatatt gacattaagt actgaagcat atattgtttc
      421 agttcttttt tgttaaaatt aacttatttg acaaagatat catagtttaa caaagctttt
      481 aatttaaata tattgatgta taatataatt aaaatataat agcaaaaaga aaggaggaat
      541 atcatgagca tactcctaaa aaaagcaccc aaattagcaa aacatattat aacaagtttt
      601 tatataaatc gagatatcaa tgaagtatta aaataccttt gtgaaaatgt tacatggatt
      661 ggaccaggag aacaggaatt tctcacttct tttaatgaaa taaaaaatta tttttatgca
      721 ggtcaagatg aaattccttc ttgtgatata aataatgatg tatttgaaac tgtaagtgaa
      781 gatgagaata gatgtatggt attaggaaga tatactgttc gaactaaaga aaatgctcaa
      841 atgattttag aggtaaatca acgttgtaca tttgagataa ttgaggatag aggaaaatta
      901 ttagtaaagc acatgcacat atcaaaccct tatggtgaaa tgcaacttga tgaatacttt
      961 cccactaaaa ttggaactca aagttatgat tatttgcaaa gattactaaa ggaaaaaaca
     1021 gaagttattg aaatgattac aaataatatc aatggtggct taaaaggtag caatgatgat
     1081 agtacatact catttttcta tgtcaatgag ggattgccca aaatattagg atacacatat
     1141 gatgagttca tggaaatgag tggtggttca gcagtgggtg ctgtatatcc accagactta
     1201 cctaaagcac tagaagattg tgaacgatgt tttgcaaaag gacctacata ttcatctgaa
     1261 tatagaatcc gaaaaaaaga tggaacatta atgtgggtat tagattctgg aatgaaatcg
     1321 ttaaatagtg atggcatagt aaaaattaat agtattatta ctgacataac ccaactaaaa
     1381 aacatagaat ctgaactaaa attagaaaga gagcgatatc gtattgcttt gcaaaatatt
     1441 acagatatca tgtttgagta tgatattgaa aacgataccc ttataaaata tcaacgtgta
     1501 gaaattgaca aaaaaattga attagaaaat tttgaaacaa aaaattactc caaactatta
     1561 gaatctggta aaattattca ccttgatgat attggaaaac tactagaagt acttcatgga
     1621 aatcttcgtg aaacaattga gataagacaa gtaaattcat taactaaaaa tgaatggaga
     1681 tggattcgtg ttcaatgttc agttatatat gatagtgacc ataaccctat caaaacaata
     1741 ggcgttttaa aagacataac tgaagataaa tctaaattag aaagcttaat taatcaggct
     1801 caacgtgacc cactaacaca attatataat caaagagtat cacaaaatct tattgaagag
     1861 tatctatgta gttctgattc caaaaataat gatgctctat tagttattga tattgatgat
     1921 tttaaaactg taaatgatac ttttgggcat ttagagggaa atgaagtttt agttgctgta
     1981 tcaaaaatac tattacataa cacacatgat aaagacatag ttgctcgaat tggtggagat
     2041 gaatttacaa tatttataaa atcattaaca aaggatttaa taataaaaat tactaatgat
     2101 attttaaatg atgcaagtaa aataaaagta aaggacaatc ataaaataac attaagtatt
     2161 ggtattgcct tcactgatga ttcaactaaa ttatataaag atttattttc taaagctgat
     2221 aaagctcttt atctatcaaa agcagatggc aaaaattgtt atagtacata cgaatgataa
     2281 agctatttat acgaccaaga atgaaccttt agatttaatt tcatagtcta acaatcaaag
     2341 agttgaagct aatcttatag ctagtcaact agttcctaga aaaaatttag acaatatatt
     2401 tattgatact gctcattttt ctaaagatca aatttatata aaaaattaaa cttttatgta
     2461 catacagata ctaatgataa acatttagca ttagtatctt ctatcttaaa ataaaatgat
     2521 aatttatcta tataaaaaag tgctttgttg acagagttca cttttttata taggtacaga
     2581 atttaagtat aaatacatgt tttattctat acattttagt taatgtaaaa tttcttgatt
     2641 gtactctcta acttttcaac attatactat ttacatacca cttagttaat ataaaaccaa
     2701 aagttggaga aatagaagtg ccattcacat tcgaatttac ataccactta gttaatataa
     2761 aacactttaa taattctacc tttactagtg ctaaagtaat ttacatacca cttagttaat
     2821 ataaaaccag gaacatatgg caaactataa ggaaaacctg caaatttaca taccacttag
     2881 ttaatataaa accctctgtg actccatatt ttttcataat atataattca tttacatacc
     2941 acttagttaa tataaaacat agaagttgtt gcatataact acattgacaa gttattattt
     3001 acataccact tagttaatat aaaacttttc tactttttta atcatttaat cacctgctta
     3061 aaaatttaca taccacttag ttaatataaa acttttgata ttctaaagaa gtgacaatga
     3121 tgtgacacaa atttacatac cacttagtta atataaaact attatgtagt catatatttc
     3181 aaactctaat ttcgtattta cataccactt agttaatata aaacccattt gacccatgca
     3241 tattgacttc caccttcctt atttacatac cacttagtta atataaaact ttacaatata
     3301 gacgtacata tacttctgaa aaacctcttt ccataccact tagttaatat aaaactttac
     3361 cccaaaataa actgggcatt ttcaatactt acacatgtgc aactctctta aatttgcagt
     3421 gaaccatgag tagtgcaaat gataaagttt atcatatacc ctcaacacct tatatttcaa
     3481 gcattaagcc ctactttgta acaaatatcg ctcactgcaa aactatttac atttttatta
     3541 tatcataaat atattatttt tgaatatctg taccaatttg tggtataata aaaacaagaa
     3601 gaactacaat ctatttagac gtagagtgaa gttcaaatac taatgtttat tctttttgaa
     3661 cttgattttt agttcaaaac taaagtcact cctgctgaac tatacccact gttttataca
     3721 gttaaaacat gtaaaagtag ttataaagta ctagtgtaac tcattagtac tttttttatt
     3781 atattcatat aattaatatc tatattttaa agtttact
//
LOCUS       DAELSY010000241         3817 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.243, whole genome shotgun sequence.
ACCESSION   DAELSY010000241 DAELSY010000000
VERSION     DAELSY010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.243"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            232..1125
                     /locus_tag="KPG09_003675"
     CDS             232..1125
                     /locus_tag="KPG09_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBG0968230.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     ILKLEEGDSHHHSNCGCGGNK"
     gene            1125..2519
                     /gene="gltA"
                     /locus_tag="KPG09_003676"
     CDS             1125..2519
                     /gene="gltA"
                     /locus_tag="KPG09_003676"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBG0968231.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLNINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKTKVNA"
     gene            2885..>3817
                     /locus_tag="KPG09_003677"
     CDS             2885..>3817
                     /locus_tag="KPG09_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HBG0968232.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLRPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKL"
BASE COUNT         1509 a          385 c          740 g         1183 t
ORIGIN      
        1 acaaattaga cgtatgatat agacttagtg tttattggca tttacaagaa atttgttaat
       61 atcaactatt tgtagtttgg gcagttataa ttactatagg ggggatgtcc aatacaaaac
      121 aatcaaccaa gaactaatat tatattataa agagttatag ttttgtgtgt ttatgttttt
      181 aattaaattt aaagtgggaa aaatatattt gttagaatgg agaatagcga gatgagtaat
      241 aaaatagtta gtaagagaca attaacagat agtatttatt taatggaaat agaggcacct
      301 agagttgcaa aatcttctca accaggtcaa tttataataa ttaaaaatga tgaaaaggga
      361 gagaggattc ctcttacaat agctgactat gatagagaaa aaggaacagt aactattgta
      421 ttccaaacag ttggtgcttc aactaaaaag ttagcaatgt ttgaagaaaa cgattttgta
      481 atggattttg tagggccatt aggacaagca agtgaattta tacatgaaga tatagaagaa
      541 ttaagaaata aaaagatatt atttgtagct ggtggagttg gttctgctcc agtttatcca
      601 caagttaaat ggtttaaaga gcatggatta gatgtagatg taataattgg tgcaagaact
      661 aaagaattaa ttatattaga agatgatatg aaaaaagtag ctaaaaatgt atatgtatca
      721 acagatgatg gtacatatgg atttaatgga agagttacag acctattaaa agatttagtt
      781 gataatcaag gtaaaaaata tgaccaagca atagtaattg gaccaatgat aatgatgaaa
      841 tttatgtgcc aattaactaa agaactaaat atacctacta tagtaagttt aaatacaata
      901 atgatagatg gaacaggtat gtgtggtggt tgtagagtta gtgttggaaa tgaaactaag
      961 tttgcatgtg tagatggtcc tgaatttgat ggtcatttag ttgattttga tcaagcaatg
     1021 agaagacagt ctatgtacaa gactcaagag ggaagagcta ttctaaaact tgaagaaggc
     1081 gatagtcatc atcatagtaa ttgtggatgt ggaggtaaca aataatggat gctaaaaaag
     1141 taaaagtacc agttagagaa caagaacctg ctgtgagagc aacaaatttt gatgaagtat
     1201 gcttagggta taataaagaa gaagctatgg cagaagctaa tagatgttta gcttgtaaaa
     1261 aaccaaaatg tgtgggtgga tgtccagtag gaatcgacat accagggttt ataacaaaaa
     1321 ttaaagaaga tgatatagaa ggtgctgcta aagtaatagc taagagcagt tcattaccag
     1381 ctgtttgtgg tagagtatgc ccacaagaaa gtcaatgtga aggtgtatgt atacttggaa
     1441 taaaatcaga tgctgtatca ataggtaaat tagaaagatt tgtagcagat tggtcaaaag
     1501 aaaatgacat aaatttatct gatacagagc ctaagaaaaa tcaaaaagta gcagtaatag
     1561 gaagtggtcc agcaggtcta gcttgtgctg gtgatttagc taaaaaaggc tatgatgtaa
     1621 caatctttga agcaatgcat gagccaggtg gagttttaac ttacggaata cctgagttta
     1681 gacttccaaa acaagctgtt gttcaacctg aaatagacaa tataagaaaa ttaggtgtaa
     1741 aaatagaaac taatgtaatt gttggtaaaa caattacagt agatgaactt atagaagatg
     1801 aaggatttga agctatattc ataggttcag gagctggtct tccaatgttt atgaatatac
     1861 caggggaaaa tgcaaatgga gtgttttctg caaatgagtt tttaactaga gtaaacttaa
     1921 tgaaggctta tagagatgac tatgatacac ctattagttc tggtaaaaaa gttgctgtag
     1981 taggtggtgg aaatgttgct atggacgctg ctagaactgc attaagatta ggttcagaat
     2041 catatatagt ttatagaaga agtgaaaaag agcttccagc aagagctgaa gaagttcatc
     2101 atgcaaaaga agaaggcatt atatttaata cattaacaaa tcctaaggaa attttagtag
     2161 atgaaaatgg atatgttaaa ggtatggttt gtataagaat ggaattagga gagccagatg
     2221 attctggaag aagaagacct atagaaatag aaggttctga atttgtactt gatgtagaca
     2281 cagttataat gtcacttgga acaagcccaa atccactaat atcttctaca acaaagagtc
     2341 ttaatataaa taagaagaga tgcttaataa cagatgaaaa tggtcagact tctaaagagg
     2401 gtgtatttgc aggtggagat gctgttactg gagcagcaac agtaatatct gcaatgggtg
     2461 ctggtaaaac agcagcagca tcaatagatg aatatttaaa aacaaaagtt aatgcttaat
     2521 ttgtaaatta aagagagggt gtctcaaaat gaacttttta gttcatgagg cacttttttt
     2581 gtacaaaatc aaatatgttt taatcatttc aacaatgaaa atttcattcc tgtagaaagt
     2641 ctaactttta agtcttagta caatctctat tttgatgtag gcactttttt agatggttta
     2701 ataatttatc agttattatt gtaatatttg atactaagta acataacttt gtctaactat
     2761 gataaaatag ttatacaata aaggtcttgt aaggagtgaa taatgtgtgg ctaataaaat
     2821 cagatttgtc aaatctaaca ttaaataata gaaaaataaa ttgtgtaatt aatggtattt
     2881 taatatgaaa aaaagagaaa aagtatttga tgggctagtt tttaagcata tattaatctt
     2941 attaatacta ttagttttga ttgatttaac aagtaccatc atatatgcaa gtaactctat
     3001 ggaaattact tctagaaata tgattgaggc atcaaaaaga gaacttgaaa actatttaga
     3061 agtaaacata tctttattaa aagctcttag tcaagatgat agattttcta atggtgaaac
     3121 atcactaatt gaaaagggaa aattattaag gccatatcaa aaggaatata atctttttat
     3181 gattggtata acagatacaa aaggaaatgc ttcaagtaca tatagggaaa atgttaactc
     3241 aataaaagat aagcctagtt ttgagaaagc aattaaaaca aagcaagtag tagtttcaga
     3301 tattgaagtc agtaatgtaa caggtgacaa agtatttatc atatatgtac caattgtaaa
     3361 aaataatgag atgattggga ccatttttgc ttctttttat tttcaagatg taaataattt
     3421 aataagtaga agcaattttg atgattctat taaatttcta atgatagata aagattatac
     3481 tattatttct catccaaaca aaaaatatgt aaatgataaa agtaagatgc ttgatttaga
     3541 agggaatatt ataggaacca ctaaaagtga aatcttaaaa aatataaata aaaaatgtca
     3601 aggtgatttt ttatcttggg ataattggag attatataat gtaaaatata cgaacataaa
     3661 gtggactaat tggactttag tatcaaaatg taatatattt aagaacttta aaaatctaat
     3721 agttaatttt atgataaagt tgtattttta tatagtaata tttatgatat tgtggaaatt
     3781 aagtaatgct aaattaatag aacaacttaa aaaattg
//
LOCUS       DAELSY010000242         3785 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.244, whole genome shotgun sequence.
ACCESSION   DAELSY010000242 DAELSY010000000
VERSION     DAELSY010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.244"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            216..593
                     /locus_tag="KPG09_003678"
     CDS             216..593
                     /locus_tag="KPG09_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0968233.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            586..1620
                     /locus_tag="KPG09_003679"
     CDS             586..1620
                     /locus_tag="KPG09_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG0968234.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENAVGNNITQIDISTDNNLMLYVAYLWLVVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDVNCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLTDCSLKDKKEYALALIKSIKLSKNYNSGILTTEFNKTSLE
                     KRLDSIVKKKGKNGILVLLILITLFSVTYFNFESISFDNSNSLRNMGILQKSGVDLKE
                     EDDISMRVIN"
     gene            1686..2273
                     /locus_tag="KPG09_003680"
     CDS             1686..2273
                     /locus_tag="KPG09_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBG0968235.1"
                     /translation="MKNLKKILIITGCLTLISTSLVFANTTTDVDTSKDDDSIISSCT
                     ITGYEFTYENAPQSVKFDYEKKCEALNIKPSKNDIIFVNKDSIDANDVENYVEPKAYI
                     IRYKKGYVFEVTGPKSYTLNVSNTVVGYNRVESGNPVHLVQLLCRTLDGSSIDDDSEF
                     GPATYNAVKSLQGRLGLTRDGIVGRSTWDAAGRRL"
     gene            complement(2651..3412)
                     /locus_tag="KPG09_003681"
                     /pseudo
     CDS             complement(2651..3412)
                     /locus_tag="KPG09_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612258.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1418 a          401 c          536 g         1430 t
ORIGIN      
        1 ttttaatgtg taaataacat tctacactat tgtaattaaa tatgtaaaaa gtaaaatatt
       61 taaatagata tattttaaaa tctaactttt aatattgtaa aaaaattaga aatttaacaa
      121 aaagtacaaa aattcacatt tttttcataa agtgtttgac taaaaaattc acatgttgta
      181 gaattaaggt agcaggagtt ttgagggagg tgtaaataat gataagaaaa ataccacaag
      241 cagaactaaa agtaatgaaa ttcatttgga aagtagatgt tacagtgaca tcaaaagatg
      301 ttattgaagc tatggaacaa aaatatggct ggaaacaaac tactacactg acacttttat
      361 ctaggctggt aaaaagagga ttcttagatg ctcaaaaaat agacagatat acacattaca
      421 caataagagt aaaacacaaa gattatttaa gttttgagac aaaggatttc ctaagtaata
      481 tacatgatga ctctttgaga agcttaattt cagcattaca tgatgatgaa aatataggta
      541 aagataagct agattttcta gaaagttatt ttgataactt aaaggatgaa taattatatg
      601 ctatatgttt ttgaaaatct tataaagaca actattgtat gtagtcttgg tatttgtcta
      661 ttattatttt tgaaaagata tttatttaag aagtttagta agagattcaa ttattatatt
      721 tggttgataa tagtctttag aatgttactt tttctattta attatacaat agtgtatgaa
      781 gtaaaagaac ccaaagaaaa tgcagtagga aataatatta cacaaattga tatttctaca
      841 gataataatc ttatgttgta tgtagcttat ttatggttag ttgtaactat agtcattgca
      901 gtttatacat ttataaagta tacaagattt aaaaatctag ttgttgatgt atcttatgat
      961 attgaagata atgatgtaaa ctgtatctac aaaaacttgt tgaaagaatt aaatataaaa
     1021 aagaaaattg aattaagagg ttctgatgaa ttgataagcc cagcaggcat gggattattt
     1081 aaatcttaca tattcttacc agactatcct tatagtaaag atgaactaac ttggattttg
     1141 aaacatgaat taatgcattt taaaaataaa gatattttaa taaagttttt agtgttatct
     1201 gttagaataa tatattggtt taatccactt gtttatgtaa tgagcaataa agtaaatcta
     1261 gattgtgaat tatgttgtga tgaaagtgtt ttgactgatt gttctttaaa ggataagaag
     1321 gagtatgcat tagcacttat aaagtcaatt aaacttagta agaattataa tagtggtatt
     1381 ttaacaacag agtttaataa gacaagtctg gaaaagagac ttgacagtat tgttaagaaa
     1441 aaaggtaaaa atggaatatt ggtgttattg attttgatta cattattttc agttacatat
     1501 tttaattttg aaagtattag ttttgataat tcaaattctt taaggaatat ggggatttta
     1561 caaaaatcag gagtagatct taaggaagaa gatgatatta gcatgagagt tataaattaa
     1621 tagattaatg tatgtaatga aaaggtatat tgtcgtatta ttaataaatt aggaggaatt
     1681 atgaaatgaa aaatttaaaa aaaattttaa taattacagg ttgtctaact ctaatatcta
     1741 ctagtttggt atttgcaaat actactactg atgtagatac atcaaaggat gatgattcaa
     1801 taataagttc atgtactata actggttatg aatttacata tgaaaacgca ccacaatctg
     1861 taaaatttga ttatgaaaaa aaatgtgaag cgctaaatat aaaaccatct aaaaatgata
     1921 taatttttgt taacaaagat tctatagatg caaatgatgt agaaaactat gttgagccaa
     1981 aagcatatat tattagatat aaaaaaggtt atgtttttga agttactgga cctaagagtt
     2041 atacattaaa tgtaagtaat actgtagtcg gatataatcg tgttgaatca ggtaatcctg
     2101 tgcatttagt tcaactttta tgtagaacct tagatggttc aagtattgat gatgatagtg
     2161 aatttggacc tgctacatat aatgcagtta agtcacttca aggaagactt ggattaacaa
     2221 gagatggaat tgttggaaga agtacttggg atgcagctgg aagacgtctt taaaaagaaa
     2281 gctattgcac agataatttt aaattgctga atctgtaaac atttacatgt attctttaaa
     2341 taaaaattta ttttttaagt gataattaaa tttttactaa tagggctatg gctttaaaaa
     2401 tcatagcctt attttgatat cttttccatt tttgcaaaag ctattaaccc tttaagaaaa
     2461 tttcttgttt ttttgaaaaa tttctttctt caaaataaat acttaaatga gctaaaattt
     2521 gagcacaatt tctaattttc tgagtccatt tttaagaaat atatatggtt gttaagtata
     2581 gtgatttgtg caaataatta tccaaagtaa aaatagattt caatttagta acttttctta
     2641 ataatctatt ctaacttccc attgaatttg tagtatatat cgatgtattt atttcttgag
     2701 agtacttaaa atatgttact aattcattcc aattgtttca ccaacctctt aatactattg
     2761 tatattgatt gtactatttt ttctctagtg atagctcact taatgctacc tcttctgtaa
     2821 ttgtagcata tctttcttta aatcttttac agattctttt ctatttttat atgatacata
     2881 atttaaagta tttttaattt gatgcacaac atatctttgt acttaagtat caggaaatat
     2941 cgctttaatt gcattagaaa attcaactag tctatctata gaagctgcta ttaatacatc
     3001 tttaattccc atgtttctag catctataag cacttttagc caatactttg aaatttcatt
     3061 ttctccaatc catattctta aaacctcttt tatgctacat atagctatat agactgtttt
     3121 gtttattatt cgacctcact tctaacttta taatgtatag catctataaa tacaattgaa
     3181 taaattatat taagaggtct aagttaccat ttatgagtta aaggaataac tttatcagta
     3241 gttttagtta ttagtgaatg agataatatc aattccataa gtacttttaa gatgacttaa
     3301 tatattttga gtagacatgc cttttgagta tattccaatc acttgatttt ttattcctaa
     3361 tatatcattt tctatcataa cctaaaagtt cattaagctc cgcttctaac atttaatgga
     3421 ttgtactttc aaataaatct tttagtatat cttgaatatc tgtaattgtt ttggcatcat
     3481 attcttcaat aaattttttt aatattttag tttttgttaa acataaaaag tgaacatctt
     3541 cctttgtata gtagtgatat tttaattatc acaacttttt agaagaatac aaactttatt
     3601 ttaaagacac tgaaaatggt gtttttatac ctttatttgc tatgtataat tttacttata
     3661 acataaactt atataaaaca gatattttaa taacacaaag gtacaattag gagattgtta
     3721 aaatttatta gatattactc tagtttattt taaaaaaaag tattataata ttccttgtgt
     3781 gaaaa
//
LOCUS       DAELSY010000243         3742 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.245, whole genome shotgun sequence.
ACCESSION   DAELSY010000243 DAELSY010000000
VERSION     DAELSY010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.245"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..644
                     /locus_tag="KPG09_003682"
     CDS             <1..644
                     /locus_tag="KPG09_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBG0968236.1"
                     /translation="LSGVPQSKVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNK
                     KKEFDNSMDNLKNSLQILNKNKSVEYVLNIKGDKNILKKALEMIKNAKQEIIISCPYE
                     EILELKKELLIAYKKNINIEILLLENKSLKGLDNIHTHGGNKTTLENKMLYKGIILIV
                     DNDEILTGNLSEGSEAISIWTKNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            631..1512
                     /locus_tag="KPG09_003683"
     CDS             631..1512
                     /locus_tag="KPG09_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG0968237.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNVADFKVQFFTPGLEVDLCGHATIGTFHALLEEGRLNTNKSIFYQETK
                     AGVLSVELKEINNENIFMMEQNTPEFENLDNYRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(1738..3093)
                     /locus_tag="KPG09_003684"
     CDS             complement(1738..3093)
                     /locus_tag="KPG09_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG0968238.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKVEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         1456 a          555 c          480 g         1251 t
ORIGIN      
        1 aattatcagg tgtaccacaa tcaaaagtct atgaaaatat aacaaagcta ttaaacaata
       61 atataattat caatgttgga actgacccta ttaagtacat acccataaac cctaatgaat
      121 tattaaaaaa taagaaaaaa gaatttgata attctatgga taatctaaag aatagcttac
      181 agatattaaa taaaaataaa agtgtagaat acgttttaaa cataaaagga gataaaaata
      241 tattaaaaaa agcattagaa atgataaaaa atgcaaaaca agaaattata atttcttgcc
      301 cttatgaaga aattcttgaa ttaaaaaaag aactcctaat tgcttacaaa aaaaatatta
      361 atattgaaat actactttta gaaaacaaaa gtctgaaagg tcttgataat attcatactc
      421 atggaggtaa taaaactaca cttgaaaata aaatgcttta caaaggaatt attttgattg
      481 tagataatga tgaaatattg acaggaaact tatcagaagg cagtgaggcc atttcaattt
      541 ggacaaaaaa ctctaacatt ttatatattg gagttcaata cataaaacat gaaatttata
      601 tttcagaaca aataaaagga ggttataaaa atgaacaaaa ttaagattaa acaagttgat
      661 gcttttacaa ctataccttt tggtggaaat cctgctggtg tcgttactga tgcctctaac
      721 atttctgatg aaattaagca gaatgtggct aaagaaatga atttatcaga aactgcattt
      781 gtttcagagt caaatgttgc agattttaag gtacaatttt tcacaccagg tttagaagtt
      841 gatttatgtg gtcatgcaac aataggtact ttccatgcac ttttggaaga aggaaggctt
      901 aatactaata agagtatatt ttatcaagaa acaaaagctg gtgtattatc tgtagagctt
      961 aaggaaataa ataatgaaaa tatatttatg atggaacaga atacacctga atttgaaaat
     1021 ttagataatt acagaaaaga acttgcacaa atgataggtg tgcatgaaga tagacttctt
     1081 gaatatccta ttataaaggt aaatactgga ctttggtggc ttgtatttgg tctaaaatct
     1141 ttagatgatt taaaaaatat ctctccagat ttaaataaaa tcaaaaaatt tagtggtgat
     1201 aataatataa ttggtataac tccattttgt atagaaacta tagataaaaa gtgtgactac
     1261 catttaagat ctatagcacc atatgttgga gttacagaag accctgtatg tggaactggg
     1321 aacgcttgcg tatcatctta tatagtacat cacaatattt tagataaaac tagttttatt
     1381 ggtgagcaag gtaactttat aaacagacct ggaaaggtat atgtaaatat aactagagaa
     1441 aacaatgaca taacttctgt aaaaattgga ggaagtgctt atactgtttt aagtggtgaa
     1501 attatgtatt aatcccatac ctaataaaaa caaataaaaa tactagctaa ctaaagctag
     1561 tatttttatt tgttattttt atttaataat cacactctaa caaatattat aattatatat
     1621 atcataatat tgtatttaga agtaaaattt tatttttatt attaattagt tataaatcaa
     1681 tctaatacaa cataattaca gtagattaat ctataaaaca taataattac ttactgatta
     1741 tttatcatcc tcaacaagtc tacttaatgt cttatcagat aagaataata atatcgctgc
     1801 aacaaatagt attactggaa ttatagtata tactttaatc atatcaaatt taagagtaaa
     1861 tgcatatata tatccatatc ctttaccagc tataaaagta gcaaatgtcc atactcccat
     1921 aagaacacct agaagcttct taggtgcata cttacttaca aaagagttac caagtggtga
     1981 gaataacatt tcaccaagac ttaaaagaat accaaacatg attatccata gtatactagc
     2041 tttagcagtt tcaggagctc caactcctct tgaaaattct gcaccaacaa gcattaagaa
     2101 tgcaagacct aagaatatta atccaagacc tgtcttttta aataaactca tatctccttg
     2161 tggtctacta gcaagtttaa accataatgc tccaagaact ggtcctaata ctatacatac
     2221 aagtgaattt aatgagtcaa accaagcaag aggaacatca aatcctccaa cattcatgtt
     2281 tacaaatgct gcaccataat cataaactgc aagataagtt agataccaga atacccagaa
     2341 aattacagag aagaatgata taagtataat agcccaaatt ctcttcttat ccatgcttgt
     2401 aagaggagac ttatcttttt cttcaacttt ttcagcaact acaccttctt taaaaggtct
     2461 ctttcctgca tttccaagga atctccatcc atatatgaac catattgcac ctattacaca
     2521 aagtacagct gctataaaga aacattgaga aaatccaagt acaccatttt ttgcaaatgt
     2581 tttaaggtaa agaataccaa ctgctatagt accaataaaa gaacctatat taacaaaaga
     2641 atattgtact gaaaatgctg tatcaagttc ttcttgacta tcaaaaagct gtccatttac
     2701 cgcagatacg ttacctttaa agaatgcagt accaatcgaa actaagatta tcattgcatt
     2761 taccatagaa actgtagttg caatagaacc aagatagtaa cctaccccca tgataagcat
     2821 acctataggt atagtatatc tagctcctat ccatctatct gatatatatc ccccaattat
     2881 aggtgcaagg tatgtgaatg caacaaggtt ggcttgcatt atagcagctt gtcctttatc
     2941 tattccaaga ccaccatgaa ctatagctgt tgtaaggaat atatatatta agtactttga
     3001 agcataatat gctgctcttt caaatgagaa agttgttgag cagatataaa atcccatagg
     3061 aaatttaccc ttctttttta ctgttgagct cataatttta aatcctttct taattgtatt
     3121 tttatttttt acgttttgtc caaattcttt cttttatgaa agatataata ttattatttt
     3181 gtaaaacaat ttgtaattat tattaagata attgcgcatt tatttgtaga gtacatattc
     3241 tcatggaata aaattaatac acttcttaat cataaattta aattatataa attcactatt
     3301 ttcagatata gttataaatt aatatattct catctaatac catgaaaaaa ccttcgccat
     3361 atgcaattct tattttttac gtgatagtat tttacaaact aaaatccccc ttttgtagca
     3421 acttctgtaa ttacatttta gtgctttttt aaaggagtct cgaataaagc aacatatatt
     3481 ttgttcaaat aatgttttta tttacttttt aaatttatat attacttcac tcaaccaatt
     3541 ctcaacttaa ataaataaac tactatctat tcacagatta gacctatttc ttacacatta
     3601 gtttataata aacactgtga atctaaacat aaaaactgtt attttaaagt aattacccat
     3661 atattcgctt tgatactatt tattgaggtt atactcttta tttataatat attatttata
     3721 aaatacctca aaatatatta tt
//
LOCUS       DAELSY010000244         3735 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.246, whole genome shotgun sequence.
ACCESSION   DAELSY010000244 DAELSY010000000
VERSION     DAELSY010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.246"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(125..520)
                     /locus_tag="KPG09_003685"
     CDS             complement(125..520)
                     /locus_tag="KPG09_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG0968239.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            661..1053
                     /locus_tag="KPG09_003686"
     CDS             661..1053
                     /locus_tag="KPG09_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0968240.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            complement(1147..1563)
                     /locus_tag="KPG09_003687"
     CDS             complement(1147..1563)
                     /locus_tag="KPG09_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG0968241.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(1578..2489)
                     /locus_tag="KPG09_003688"
     CDS             complement(1578..2489)
                     /locus_tag="KPG09_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0968242.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYNTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            complement(2671..3693)
                     /gene="splB"
                     /locus_tag="KPG09_003689"
     CDS             complement(2671..3693)
                     /gene="splB"
                     /locus_tag="KPG09_003689"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBG0968243.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
BASE COUNT         1237 a          648 c          429 g         1421 t
ORIGIN      
        1 aataactaaa aatttttaca tattcattat ttaaaaatct ttttattgca cttaaattaa
       61 atctatcaat atttcagtaa taacgttttc ttaattttca ctatggatgt cgattcaatt
      121 ataattaaag ctctacatca atctttttac cactcattga acaacaacta acttttgcct
      181 tatcaataga gaatatttca aacttaccat ctcccacaga gtacatctga cctaaacttg
      241 gcattatttc aagtgcctta gcttgtgttt cttttgatgt aacaaatact gcttcaccta
      301 aaatacgtag agtattaagg ttttggtcta tacaacaaag ttcaaccttt ggattaaaaa
      361 taagttgctt aaacatatcc ttttgattag atgtgcaaaa tgtaagttta ccattgtatt
      421 ccataacaaa tcctaaagga cgcacatgtg cttgattgtt ctcatcaact gttgctaaat
      481 aataaactcc tgccttgttc aaaaattcta atgctttcat aattcttaca ccccttttaa
      541 atacttgtat taaggattat cctttgatat aattttaata taaaatacag aaaaaacaag
      601 tatgcagttt tttctgataa agtataaaaa agatactact ataactaagg agtaattaaa
      661 atgagtataa atactgaaaa aaatataact aaaaacgttg aagaattaac ctgtccaatc
      721 cgttatgcat tagacattgt cggtggtaag tggaaattac ctattatatg tatgttagca
      781 gttgaaaatc caattagata tagtagcata aaaagaaaat tggatggcat tacaaatact
      841 atgctagctc aatcattaaa agatttagag tctactggta ttgttcatcg taaacaatat
      901 aatgaaattc ctccaaaagt tgagtataca ttaacaagta aaggtaaaag tattgtgcct
      961 attttacaac aatttgctaa ttggggtgct actaatatgc aagaaaaaaa cacatgtgga
     1021 cttagttgca aagaatgtag aaagataaag taaatttact aaattattca tattatagac
     1081 tatatattaa tttatctgat tgaatataaa aacaggatga cttatatatt catcctgttc
     1141 tatatgttaa gctctaaatt taattagttc aagcatatcg tttaatacca ataaactatc
     1201 tacttgaatc attagccatt tacttccatt aaaaaagttt acctcattat atattttttt
     1261 gatataatca caacaagtat taataaataa atcaacctct actctattgc tttcacttac
     1321 aataaccaaa gctttaaaat aaccttcttg tggatataaa gtacatagtg atttacctct
     1381 ttttttatat tttacattcc aacctctttg cattgaacat ttactgtatt caagcttagg
     1441 ttctacatta taagtagaaa ccaaagtttt atttaacttg tcccataatg gattattaat
     1501 aaattcatta atttgctcaa atgtaggttt agtatcttga aaaaacgcat cttgccaatt
     1561 catattcatc atcctttcta ttgttcaata ggaaccggaa caatccaatg ttccagatat
     1621 gctgcttcag gtgtagtggg gtaatacatc tcaattgcaa aatcagtagt tgttaaatta
     1681 tgtttcttca tccatctttc cataaattta tctgctttaa aaacagctga attaactaac
     1741 tcattaaagt tttctgcctc aaatccacat actaaatatt ctttagctgg caactcaaaa
     1801 gtattatatc cttctactga tgtacctgac tctacctcta cacctgccat atacatacaa
     1861 tttccttctt ttgcatttcc catataaaga gcagcaaatt catttccacc tggaatttga
     1921 tttggtatat tatgtctttg acttccaagt ttcatccata attcctctac tattgcaatt
     1981 ccagttgttt caccacccac caactggcaa ataggtactt ctatttctat tccaacaaat
     2041 gtccttggat tactcaatgt cttacgattg acttcaatca ctatatcatc tgcaatcagt
     2101 ggaacacctt catcaaccat agcataattt aaaattaatt ctggcttaat aaagtgattg
     2161 agttttactg gatttgctct atatttttct ggagttaatc catatgcttt tttaaaggaa
     2221 cgtgtaaatg tctcatgact agcaaatcca aaatttaaag ctacatcaag tatacgattc
     2281 ttatgttctg ctaagtattc agaagctctt gcaagacgac gaagctttat atactccatt
     2341 acagtcttat ttaccaatct tttaaatagt ctttgatagt aaaatggtga taaatttgct
     2401 ttatttgcta attcattaat tgatatttct tctgataaat ttgcttctat taaatccaat
     2461 gtaatttgaa tagactccca tgcatgcata ctataactcc tcctgatatt tttgttactc
     2521 ttacccttta tagtatcaaa actagaaaaa atgttcttga tagatagtgc cctttttaat
     2581 tttcttatat gatagtgcta aatctcattg ctttattata aacataaaat atataaaaat
     2641 aaaatatagc aaatctttag gtaagatttg ctatattata tattttatta aactattttt
     2701 aaatactcca tttatttcct taagaaagaa ctctttcata tcatctaatt gttctttatc
     2761 atacacgtac ttaccatatc caaattgacc gtatttaaac tttctatcct cattttccat
     2821 tggcaatagt gtatctggat aaatctctag tattctattt tttgctttag ttgtatatct
     2881 atgagatata acttcaaata taattttttt atcaaaatcc tttggtaaag cttcttttat
     2941 acttataatc agatttttat actcttcttt ccaaccttca tataaaaaca caggtgctat
     3001 tatgaaacca atctgataac cagccctagc aagttttaca gctgactcta ttcttttatt
     3061 tgcagaggct gtataatgct catgttcatt tataattcta ggagtattga tactaaatcg
     3121 tatttctgta tgattattat gttcagcatc caataagcta tccacatcac tgtatttagt
     3181 aacaaatcta aatcttcctt tttcctctct tccaaaaaac tctattgttt tcttcaaaga
     3241 attagtatat ggctctactg gaactgggtc tgatgtagct gccccttcaa atatggttac
     3301 ctgtggcttt ctatcttcaa tatatttttt tgtctgttca agtatttcat ctacatttac
     3361 aaacaccctt ataaatggtt tatcacctaa ttgtgtgttc aaataacaat actcacatct
     3421 tcccatacat ccactgacaa gtggtagttg ataattagca gaaggtttac atgtttgaaa
     3481 cttcagactt tttctaatcc caactaccat agtctgcttc ccacttctat attgttcata
     3541 tagattttca cctggaatgt gttctttaat cctatttgat gatatattaa ttatctctac
     3601 atctttatta tctctaaatt tattgtatat ttttttgccc acatcagtgt ctaaagttcc
     3661 tttttcgaat attattcttt tgggattaaa catttatttc acctctactt aaagttttta
     3721 aatcgaagaa tttta
//
LOCUS       DAELSY010000245         3706 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.247, whole genome shotgun sequence.
ACCESSION   DAELSY010000245 DAELSY010000000
VERSION     DAELSY010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.247"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(85..1335)
                     /locus_tag="KPG09_003690"
     CDS             complement(85..1335)
                     /locus_tag="KPG09_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBG0968244.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLYEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDSDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1367..2308)
                     /locus_tag="KPG09_003691"
     CDS             complement(1367..2308)
                     /locus_tag="KPG09_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBG0968245.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2548..3624)
                     /locus_tag="KPG09_003692"
     CDS             complement(2548..3624)
                     /locus_tag="KPG09_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG0968246.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
BASE COUNT         1087 a          693 c          409 g         1517 t
ORIGIN      
        1 atagaattta tattccacat acgattttca atcaaaaaaa caagggctaa aaagtgaatt
       61 gtacactttt tagcccttta ttaattattc atttatttca tgtaagaata gcccacatct
      121 tactttaggt tcaaaccatg ttgacttagc tggcattatc ttatttgcat ctgctactct
      181 tattaaatct tcaatactag taggatacat tgcaaatgct actttcatat cttcattaac
      241 tcttttttct aattcttcaa gacctctaat tccaccgata aaatcaattc ttttatctac
      301 tctaggattg tcaattccta aaatagcatc aataacatag tcttgtaata tagaaatgtc
      361 taatgagtca acagggtctt ctatttctaa taaattttta tttgctttca ttttatacca
      421 cttatcacct aggtacattc cgaaagttcc ttttttgtca ggtttatatt tttcttttcc
      481 ttctatttct tctagttcaa aattttcttt tattttattt ataaattctt cctcgcttaa
      541 accatttaag tctttgataa ccctattata atccaaaacc attaaattct catctggtgc
      601 aatcattgct atgaagtaat taaattcttc tttgccagta taatttggat ttttagctct
      661 catttctttg gctacatttt ctgctgaagc tgtcctatga tgaccatctg caatgtatag
      721 ataatctaat ttttcaaatt tatctactag gttatctatt atatcatcac tatcaatctt
      781 ccatactgta tgctctatct tgtcatctgt tatgaaatca tataaaggtt cattattatc
      841 cataaaatca ttgattatat catctatcac cctttgatgt ttgtaagtta caagtatagt
      901 tcccgtatta gcatcacagt attttatgtg tttagttcta tcaatttcct tttctggtcg
      961 agtgtactca tgttttttta ttgttccatt caagctttca tcaactgata tacatgctac
     1021 tataccaact tgagctctac catccatgat ttgtttatat atatatatac actctttgtc
     1081 atcttttact agaatttctt gttttcgaaa ctcatataaa ttttctcgcg cttttaaata
     1141 aactttttca tcatataaat ctatattttc atctaaatca atttctgatt tatctatatg
     1201 caaaaatgaa tatggattcc cttctgcaat tcttctagct tctttactat tcataacatc
     1261 gtaaggaagt gctgctactt tatctactat atatttattt ggtctaatcg ccttaaatgg
     1321 tttaatcgtt gccattacta atttcctcct aattatacaa aatattttat aatgctaccc
     1381 ctattagatt gttgaagtca agaaagaaat cttgaatcac atttactatt tcgtctccta
     1441 ttctttcttg agcttctact gtagatgccc caatatgagg tgtaggagaa actcttggat
     1501 gatttactaa gtctaaattt aatgtaggtt cttgttcata aacatctata gcagctgctg
     1561 ataatttacc ttcatctaat gcttttagta aatctttttc atcaactaaa ccgcctcttg
     1621 cacagtttat taaataagcc cctttcttca tcttatttat ttctttttca gttattactg
     1681 cccccttatt cttatcaaat ggtatgtgta aagataagaa atctgcattt tctaaaactt
     1741 cttccatatc acagaattta taattgttaa aaccttgcgc ctcacccatt atgtcagtat
     1801 atataacatt catacctaat aattctgctc tctttgcaac ttcttttgca attcttccaa
     1861 aacctataag tccaagtgtc tttccattaa tttctgtacc tttatacttc ttcttctccc
     1921 attttccatc tctcatagtt acatttgcag tatttatgaa tcttgcaaga acaaataatt
     1981 gacctattgt aagctctgca acagatatac tgcttgcatt tggagtattc ataactttta
     2041 tgccattagc ttgagcatat ttaacatcta tattatccag acctactcca cctcttacta
     2101 tcaacttcaa ctttttaccc tctaatgctt tatctattac atctttagta actttagtag
     2161 ctgaacgtac tacaagtaca tcaacatctt taagtttatc tcctaaaaca tctttttcat
     2221 aaaactgctc tatcacatta aaatctaact ctctcaattt tctcgctgct tctttctcta
     2281 ttccatcagt aactaatata ttatacataa caaactcctc caatttttat tcacttaatt
     2341 tttccaattt ttatttttta aagtattttc atcaataaaa aactttactt aatttaactt
     2401 tctgtttata taattttcaa aatatttttt tattaaagtc atatcaaatt ttcctattgt
     2461 ttgttaaaaa tttaatatgt aatttttata aaatattatt tattatatat tctaaattta
     2521 tcttaaaaca tacatatttt tacaatatta tttattaaaa cctaatatat catcaatatt
     2581 atctaataat tcttgtacat cttcgacagt atagtcaccc atatgtgaaa ttctaaaagt
     2641 tttttctttt aaatctccat agccatttgc tatttggaat cctcgttctt gtaattttga
     2701 atttaaattt gatacactaa taccttgagt attttctata acagtaagtg tattggataa
     2761 gtgattttca ttggtaaaaa tttggaagtg ttttttagcc cagtttctaa ctaagtttgc
     2821 catgtcttca tgtctgttga atctgttatc taggccttca tctaatatat tatctaattg
     2881 gaaatctaaa gcgaacatgt gagaaagaga tggtgttgaa gggtattggt agtttttctt
     2941 gattaagtat tcatacattg ctaacaaatc aagatatact cctctaaaag gtacttgctt
     3001 agctctatcg attgcttttt gagaaaatgt gcaaatagac attcctggtg gtaatcccag
     3061 tgctttttgt actgatgtta tacaaatatc tattccaatc ttatcaacct cgacttttat
     3121 tccaccggct gaacttacag tatctacaca gtagattaca tcttcatact ttttaacaac
     3181 ttctccaatt tcttctattg gatttcttat tccagttgaa gtttcattgt gagttacagt
     3241 tattaaatcg tattctccgg tagaaagcac cttatcaacc atttctggcg ttgttgcctc
     3301 tcctaattca actttaaata tatccgctgg gacattatta gccactgcca ttttgtacca
     3361 tctatcacca aaggatccac aggaaaatac tgctgccttt ttggatgtac aggaacgaat
     3421 ggagccttcc attaaaccag ttccggatga cgtggataaa agtatcgtgt tgtttgtata
     3481 gaatagtttt tgcatctttt cacttatact cttttggagc atagatgcat cttttcctct
     3541 atgtcctatc attggtgtag ccatcttttg caagacttct tctttaacat caataggacc
     3601 aggaataaat aatttcttac tcattataat tccccccttt ttagtttgta atatggatag
     3661 cgtttttact gtccttattt ttaataacta taatatcaag ctgaat
//
LOCUS       DAELSY010000246         3653 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.248, whole genome shotgun sequence.
ACCESSION   DAELSY010000246 DAELSY010000000
VERSION     DAELSY010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.248"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(106..642)
                     /locus_tag="KPG09_003693"
     CDS             complement(106..642)
                     /locus_tag="KPG09_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBG0968247.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENKDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(660..1973)
                     /locus_tag="KPG09_003694"
     CDS             complement(660..1973)
                     /locus_tag="KPG09_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBG0968248.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEENIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2309..2749
                     /locus_tag="KPG09_003695"
     CDS             2309..2749
                     /locus_tag="KPG09_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG0968249.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLETQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVSLTPKA
                     KELWVTRYTCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(2934..3122)
                     /locus_tag="KPG09_003696"
     CDS             complement(2934..3122)
                     /locus_tag="KPG09_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968250.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
BASE COUNT         1246 a          639 c          352 g         1416 t
ORIGIN      
        1 cctactcttt tttctgtgct tatatataat attaaagctc tttattttca atatatattt
       61 taaattttct ctttttaata tatctttttc ttttaataaa tctctttaat tttcttcttc
      121 tatatcagag ccaaagggaa tccatgcatt accaaactct ttatctttaa ataaataagc
      181 taatccagtt atttgcttca atctaagaat ttcatcaact tccataccca ggtgctttga
      241 tatccattca tcagattttc caatttcaca aagttcagtt ataattttac tcattaaatc
      301 cacattatga gaacctcttg cacgattgtg tcgaatagta cttgccattc tttcatcaag
      361 agatttatca attactgaaa caggaagaag tccgttttct ctatcatata tatccttatt
      421 ttcaagcatt actctatatc tatgaaaacc atctacaata atatacttat ccttttcctc
      481 attataataa cagacaattg gcattgtata cccatcttct tttatacttt tatacaacaa
      541 ctgcatttct ggtggggcta ctgcatttgg attatagtcg ttagattcta tcttttcgat
      601 tggcactgcg atgattccat atacaggact tttatattcc atagcatctt aactcctatc
      661 taatgaattt tttcatattt cttcttaata tattccgttc taattctctg ttcttttgta
      721 ggaccaaatc ccataaatct acacatatgg tcatttttta aaatacaata acacattctt
      781 ttccaacttg gtatatcttt ggtaaattta atatcatccg tatcatcagg tatatcatcc
      841 aaaaatatga ctctactctt tttatctttt gtatgatttg aaactccatt taattctatt
      901 ttataacctt ttctcaacaa ttcacgtata actatttctg acaaaccacc acctgtattc
      961 tgccaaaact caatagatgt tgaaaatttt ttaatatagc tattccttat attttcaggt
     1021 aaggtatcaa gcaaaaaata tgtgaaactt ttccatgtgt gtccatatgg caatttaaca
     1081 ctacgatatc ccatagcttt tgtttttcca taaatagaag taaaattaac tccttgtact
     1141 ctagcaacca atttcaccca tacatctggg tccaatattc tgtataaatt taaactctct
     1201 tttgctgaat cattaaaagg tgatgccact ctcatttttc ttggagctac tccagcttta
     1261 tagaataagt cataaatttt attatagtca taatcaaatt tatagtgggc taaccatata
     1321 tcttctattt cccaatcata tataggagat gccgtccaga catttttaaa ttccttagtt
     1381 atccagcact ttcctttata tccatgctgt ttattcacaa tagaacaata acgttgtaga
     1441 gattctgctg ctcgtgttcc taataagcaa acaacttttg caccttcatg tatatctcta
     1501 atccatctac caaattgctt tgccaaactt tcttgtctca ttttatactt ataataagta
     1561 aaaggattat ttttttgatt tattatgtat tctttgactg gcatttctct tacccataat
     1621 tcttctttct catcatccca tggataccaa tacatttcat aatttcctac agcagttctt
     1681 gtagccattg gtaaacacac ccaatatggt tcaatatctt caatattctc ttcaaatact
     1741 ttttcaacat attcagtagt caaactatat tgagcctcaa aatcttgatg aaatactcct
     1801 attttggaac ttatattatt ctttctctta taatccaaaa ctaaatttag cagtaagcca
     1861 ctatcttttc ctccactaaa agatacatag atataatcaa attcattaaa aagataagaa
     1921 agtctttctt gaaaagcttc aaatacatcg gattctaaat atctcttatc catttgaatt
     1981 atcccctttc aattagaagt ttataaaacc ttacaataca atattattat gcttttttag
     2041 cagtaatcag cataaattat acattattta aatacacaaa tccaactaaa cacatataat
     2101 tataatgtaa ttatactttt gtattataaa ctatatttta tcattatttt acattttttt
     2161 ccaatatata taaatatcat atttttctat tagctaaaaa taattacttt atatttttac
     2221 atgttagtat aataagacat aaaaacttga catactgttg tcataattac tttgctataa
     2281 ttaaaagtaa aaatggaggt aaattataat gtatgaacgt atgcttgata aaagcaataa
     2341 accctgtttg tctcaaataa tagaatatat aggtaaagat ggatatgcac gattgtgtaa
     2401 gttggaaact tacttggaaa ctcaatataa tttatcaaga gaacttcgat ttccttttgg
     2461 aaacaattat ggttggggtt acaaatatag ccataaatct tttcatttat tttatgcttt
     2521 ttttgaaaaa ggtgcattta ctgttctgat tcagataggt gacaagcaag ttccagcagt
     2581 agaaaaaacg ttggtatctt tgacaccaaa agctaaagaa ctctgggtca ctcgttatac
     2641 ttgtggcaat aatggaggtt ggattaagta tcgtatttta gaagatgcag acttacttga
     2701 tattattcaa tatataagag caaaaaaatc tccacctaaa caaaattaac ttttattttt
     2761 tgtgatttaa atatagtgta tataaattcc attctttcca tattaaaaaa ataatataaa
     2821 aaatatctat tagtatatct tatttagtat gtcttattct actttttaca gataattaaa
     2881 aagttttatc ataaataaat cataactaaa catcctagta aatcaaaatt catttattct
     2941 tttttattag aatttttgat aacagtccct atacatatgc caattatcat tccaaaaatt
     3001 agaccataac acattgtgat acccacattt cctttagaaa tacctatagc acaacctata
     3061 gccattccaa gtatcatgcc tataaacata catataataa ctaaattgtt gtttttattc
     3121 atagtttttc tccattcaat atattttaaa ttgcctagct cgccttctta gcttaaaaca
     3181 aagaaatata aattaacttt ctttcattct aatatctgct tgtataaatg tcaaacaaca
     3241 catttcttgt cataacctcc tctttttgaa gtaagtacaa tctcccagac acaataatat
     3301 caatttccta aaaccattta agtaactttt atatttacat aatatttaca atcataaact
     3361 atattgttat tcatttatac atcataaaca ctccctccta atcattatta tacttaaaat
     3421 aatgttcatt tataataata tatctaacgc actttaattg aaaaaactaa aactttgcct
     3481 ttcattccca tatataaata agtatacttt ttgatgctct attacaaatc aactaaatta
     3541 attgatataa attatcattt tctgttgaaa ataccattta cacatgctat acttaattgc
     3601 ataaatggta ataaatgtca tatgatttta caaatctatt aataacaatt att
//
LOCUS       DAELSY010000247         3537 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.249, whole genome shotgun sequence.
ACCESSION   DAELSY010000247 DAELSY010000000
VERSION     DAELSY010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.249"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            259..636
                     /locus_tag="KPG09_003697"
     CDS             259..636
                     /locus_tag="KPG09_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0968251.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDATVTSKDVIEVMEQKYGWKQTTT
                     LTLLSRLVKRGFLNAQKIDRYTHYTVIVKHKDYLSFETKDFLSNIHDDSLKSLISALH
                     DDENIGKDTLDFLESYFDNLKNE"
     gene            629..1663
                     /locus_tag="KPG09_003698"
     CDS             629..1663
                     /locus_tag="KPG09_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HBG0968252.1"
                     /translation="MNNYMLYVFDNLIKTTIVCSFGICLLLFLKRYFFKRFSKRFNYY
                     IWLVIVFRMLLFLFNYTVMYEVKEPKENTVGNNITQIDVSIDNDFMLYLVYLWLFVTI
                     AIAVYTFMEYTKFKNLVVDVSYDIEDHDVNCIYKNLLKELDIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYTYSKDELTWILKHELMHFKNKDILIKFLVLSVKIMYWFNPLVYI
                     MSNKVNLDCELCCDESVLTDCSLKDKKAYALALIKSIKLSKNYNSGILTTEFNKTNLE
                     KRLDSIIKKKGKSGILMLLILFTLFSVTYFNFESISFDNSNYLRNIGILEKTGADLKE
                     EDDISMRVVN"
     gene            2142..2315
                     /locus_tag="KPG09_003699"
     CDS             2142..2315
                     /locus_tag="KPG09_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBG0968253.1"
                     /translation="MHLVQLLCQQINGSDIDPDSKFGPATYNQVKILQGRLGVTKDGV
                     VGKATWQAVGERY"
     gene            complement(2400..3422)
                     /locus_tag="KPG09_003700"
     CDS             complement(2400..3422)
                     /locus_tag="KPG09_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG0968254.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTLLNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNSGNGIVFIKPWNNSKIIEKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTKLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
BASE COUNT         1369 a          468 c          496 g         1204 t
ORIGIN      
        1 ttataatatt ccttgtgtga aaatatagga atttttatta aataatttta atgtggaaat
       61 aacattctgc actattataa ttaaatatgt aaaaagcaaa atacttgaac aactatattt
      121 taaaatctaa cttttagtat tttaaaaaat tagaaaatta ataaaaagta caaaaatcca
      181 cattttttca taaagcgttt gactaaaaaa ttcacatatt gtagacttaa agtagcaaga
      241 gttttgaggg aggtgtaaat gatgataaga aaaataccac aagcagaact aaaagtaatg
      301 aaatttattt ggaaagtaga tgctacagtg acatcaaaag atgttattga agttatggaa
      361 caaaaatatg gttggaaaca aaccactaca ctaacacttt tatcgaggtt agtaaaaaga
      421 ggattcttaa atgctcaaaa aatagacaga tatacacatt acacagtaat agtaaaacac
      481 aaagattatt taagttttga gacaaaggat ttcctaagta atatacatga tgattctttg
      541 aaaagcttaa tttcagcatt acatgatgat gaaaatatag gtaaagacac gctagatttt
      601 ttagaaagtt attttgataa tttaaagaat gaataattat atgctatatg tttttgacaa
      661 tcttataaag acaactattg tatgtagttt tggtatttgt ctattattat ttttgaaaag
      721 atattttttt aagaggttta gtaagagatt taattattat atttggttgg taatagtctt
      781 tagaatgtta ctttttctat ttaattatac agtaatgtat gaagtaaaag agcccaagga
      841 aaatacagta ggaaataata ttacacaaat tgatgtttct atagataatg attttatgct
      901 gtatttagtt tatttatggt tatttgtaac tatagccatt gcagtttata catttatgga
      961 atacacaaaa tttaaaaatc tcgttgttga tgtatcttat gatattgaag atcatgatgt
     1021 aaattgtatc tataaaaatt tgttaaaaga attagacata aaaaagaaaa ttgagttaag
     1081 aggttctgat gaattgataa gtccagcagg tatgggatta tttaaatctt acatattctt
     1141 accagactat acttatagta aagatgagtt aacttggatt ttgaaacatg aattaatgca
     1201 ttttaaaaac aaagatattt taataaagtt tttagttttg tctgttaaaa taatgtattg
     1261 gtttaatcca cttgtttata taatgagcaa taaagtaaat ctagattgtg aattatgttg
     1321 tgatgaaagt gttttgactg attgctcttt aaaagataag aaagcatatg cgttagcact
     1381 tataaagtca attaaactta gtaagaatta taatagtggt attttaacaa ctgaatttaa
     1441 taagacaaat ttggaaaaga gacttgatag tattattaag aaaaaaggga aaagtggaat
     1501 attgatgtta ttgattttgt ttacattatt ttcagttaca tattttaatt ttgaaagtat
     1561 tagttttgat aattcaaatt atttaagaaa tatagggatt ttagaaaaaa caggcgcaga
     1621 tcttaaggaa gaagatgata ttagcatgag agttgtaaat taatagattt ttatagtatt
     1681 ataatttatt aacaaattag gagaagtttt gaaattaaaa atctaaaaaa aatcttagta
     1741 gtcacaggga gggtgactct aatatctatt agtttagtat ttgcaaatac taataatgat
     1801 gtaaatgtat gaaaaagtaa tgaagagcaa acaacaaatt catttacaat aactggttac
     1861 gaatttattt atgaaaatgt accacaacct ataaaatctg attatgaaaa aaatgtaaag
     1921 aattaaatat aaatccatct aaagatgata caatctttat taatgaaaaa tttatagatt
     1981 catataatat taatatagat gattataata ttgaaccaaa agcatttatt attagatata
     2041 aaaaagattt aaatcgtttt gatgctactg gtcctataag ttatagcgta aatataaata
     2101 ccactgtagt aggatatgga catgtagaaa aagggaatcc tgtgcattta gtacaacttt
     2161 tatgtcaaca aataaatggt tctgatattg atccagatag taaatttgga cctgctacat
     2221 ataatcaagt aaaaatactt caaggaagac ttggtgtaac aaaagatggt gttgttggaa
     2281 aagccacttg gcaagctgtc ggagagcgtt attagaaaag aggttgtcgc actaagaaat
     2341 agggcgacaa cttttttata tagaatatat taatcagtat cttagagtct agaaatattt
     2401 tacaataaat tccatataac tctaccatct atatccttaa cattttgaca accagtcaaa
     2461 atcatagtct cacataactc attcctaacc ttctctataa aaaactccac accatcaaga
     2521 ccaccgccaa aagaagcagt aacaaaaggt cttcccatca aaacagcatc agcacccaat
     2581 ccaagcatct taactacatc aacaccagtc ctaactccac catcaacaag tattgttatc
     2641 tttcccttta cagacttagc tatatctgga agtacctcac aagtaccagg agtataatca
     2701 agtactcttc caccatgatt tgaaacaata atggcactag caccagactc tacagccatc
     2761 aaagcatcat ctacagtcat aattcctttt ataataaaag gtaatttagt agattcaacc
     2821 aattctctaa tctcatcaat tgtcttagga gaaaaaggat tttcttgaaa ttgattatta
     2881 ataagtccac aagcatcaag gtctacacca actgcaaaag cacctgcttc ttcagacaat
     2941 ctaatttttt ctattatctt tgaattattc caaggcttta taaatacaat tccatttcca
     3001 ctattatctt tcaacacatc taaattatcc agtaaaaaag tatctacatt agtatcgcca
     3061 accatagcat aaattccaga attagaacaa cctctaacaa caggctctat gtattctttt
     3121 tcggaaactt taccacccat gttcaataga gtacctgata caggtgcagc gaatatagga
     3181 gaactcattt ttctaccaaa taattctatg gatgtatctg gctttgaaac attatgtata
     3241 actctcatat ttatctttat tttttctaga ctttttctat tttcaatgaa agacgaacca
     3301 cttcctttac cacccatacc aggaacatct ccagcacaag ctaatccatt acaaatttta
     3361 catactttac atgttccatt aaagttctct ctagctgatt ttagtaagtc attgtaattc
     3421 atacaaaatc tctccttttt ataatattaa aattgagtat tactatattg aaaatatctt
     3481 aattgtaaat atagggatgt tattatgatg agctaaacta gataatacat attagat
//
LOCUS       DAELSY010000248         3521 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.250, whole genome shotgun sequence.
ACCESSION   DAELSY010000248 DAELSY010000000
VERSION     DAELSY010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.250"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(170..691)
                     /locus_tag="KPG09_003701"
     CDS             complement(170..691)
                     /locus_tag="KPG09_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG0968255.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENIIIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
     gene            819..2183
                     /locus_tag="KPG09_003702"
     CDS             819..2183
                     /locus_tag="KPG09_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0968256.1"
                     /translation="MKESNKKIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDKLHIAIKTSILWTTIFCVVLGL
                     ILVLFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            2372..3049
                     /locus_tag="KPG09_003703"
     CDS             2372..3049
                     /locus_tag="KPG09_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG0968257.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSDYKKGNPVH
                     CGDSDCIGILIIKSGTIRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQINSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
BASE COUNT         1134 a          480 c          525 g         1382 t
ORIGIN      
        1 gctcttgctc ttgctcttgc tcttaaaaag tttaacattc tccctcaatt aagtgagctt
       61 tattttaaat atacttttca gtttatatca tttgtttcat tttttctaat atctcattat
      121 taaatatgct ggtttttgtt taatgattat taagttagct tctctaattc taattcccca
      181 tattatcttt taaagaattt atccaggttt ccaatgcttg tgctagcacc aactgttgct
      241 ccaacactgc ttttgctgtc tctggtactt ctgtctttat aattatattt tcctcaatag
      301 ttttctttaa ctcttgaaca ttctcttcaa tattattgag acataatttc tgtttttctg
      361 gtgtaaggct atttaaattt actataacag cattaaaatc taaaagaata tttattggtt
      421 tgctagatgt atcaaacatt aaatctaaaa aatgtgtctc acctaattca gttaatgaat
      481 aaactgcttt ttctggcatt tttccattct tcacagtttt actttcaata tatccctttt
      541 gttctaattg aattactttt ttataaattg atggtgtact tattttaacc caacgtgata
      601 tattcctgta ttccactgcc ttttgaatat cataagcact cttgggttgt tgctttacta
      661 ttcccaatac aattaaatct attgtagcca taaaaatcat ctcctttagt tttcttacta
      721 ttgacaaata ctataaatta tagtactatt ttccatactt actataattt atagtattat
      781 aatttagact taatgtcaat agaagaggag tgttaagtat gaaagaaagt aacaaaaaaa
      841 ttgaattatt agggtctgcc ccaatctcaa aagcattgtt atctatggga ttacctacca
      901 tgataggtat gatgataact gcattataca atttggttga tgcatacttt gtgggtggac
      961 ttggaacaag tcaaatggga gctatatccg ttgcatttcc attaggtcag cttgttgtgg
     1021 gattaggatt attgtttgga aatggagctg cttcatatat tgccagactt ttaggccatg
     1081 gtgataatga aaaagcagga aaagtggtaa gtacatctat ttacagtagt attatagtgg
     1141 gtacattagt tattattgtg attatgattt ttctcaatcc aattttaaaa ttgttaggag
     1201 ctacaaaaag tattatgcct tatgcaatat cttatgctag catttatgtc gtgtcatcta
     1261 tttttaatat attcaatgtc actatgacta atattgtcac aagtgaagga gctgctaaaa
     1321 caactatgtg tgctatgtta ttaggtgctt tgcttaatat gattttagac ccgatattca
     1381 tttatacttt tggtctgggg gtaaggggtg cttctattgc aacagctatc tcacagattg
     1441 cttcaacact tgtttatgta ggatatattt taagcaagaa aagtatattt agttttagaa
     1501 ttaacgaatg ctgtttttca aaagaaattt tttcagaaat cctaaaaatt ggcataccta
     1561 cattaatatt tcaattattg actagtcttg ccattacatt aactaatatg gaggctacaa
     1621 agtatggtga ttctgtgata gctggaatgg gtgctgtaac acgaataaca tctttaggta
     1681 gtcttgttgt atttggtttt ataaaaggtt tccaaccaat tgctggttat aattttggtt
     1741 cgaagaacta cgataaatta catatagcaa taaaaacttc catattatgg actacaatat
     1801 tttgtgtagt gcttggtctt atattggtgc ttttcccaaa atctataatt tcacaattta
     1861 caagtggtga cattctatta atagaaaatg gtagtaaagc tcttagggca aatgggtttt
     1921 catttatgct atttggattc tatacagtgt attctacatt attcctagca ctaggaaaag
     1981 caaaggaagg tggattttta ggtatgtgta gacaaggcgt ctgttttatc cctataattt
     2041 tgattattcc tgtattctat gggttaaatg gtatcttata tgcacaacca atagctgatt
     2101 tgatggctgc tataattgct ggttttatgt ctataaggtt acataaggaa cttaatcttt
     2161 taaaaataaa cggtatgcat tagttataca aacctactat catcaaaaca tattactttc
     2221 tcattaatag caagaatata tattatcata ttttttctac ataaatcata ctattactat
     2281 ctgactaaat aatcctctta acaaactact tgtcaattta tctattcact tgtatcatgg
     2341 gattataaca atttaagaaa ggagatattg tatgatttca caaaatgaaa ttgatatact
     2401 tacacatagc ttgccttttt gggataaact tactgactta caaaaagaat tattaatttc
     2461 ttcagcaaat acctcggatt acaaaaaagg gaatccagta cattgtggtg atagtgactg
     2521 tattggtatt ttgattataa agtctggaac aatacgtaca tacatacttt cagatgaagg
     2581 tcgagaggtt acactgttcc gacttgatga tggagatgta tgtattttat cagcttcctg
     2641 tattctaaaa acaataactt ttgatgtcta tgtagatgct gaaacggact gcgatattat
     2701 acagattaat tcctctgttt ttgcaaaatt atctacagaa aatattcatg cagagttatt
     2761 ttcatataaa ttagctaccg aacgtttttc tgatgttatg tgggctatgc aacaaattct
     2821 ttttatgagt tttgataagc gtttggcatc ttttttaatt gatgagatag ctaaaaatgg
     2881 cagtagcact ataaatatga ctcatgagca aattgctaag tatatgggta gtgccagaga
     2941 agttgtatcg agaatgttga aatattttgc tagagaaggt attgtgtctt tatctcgtgg
     3001 tggtataaaa gtattggata aggataaact tcgttctttg acactgtaaa agtgtgtctc
     3061 aaaatgaact ttttagttca tgaggcactc ttttttgtgt gaaatcaaat atattttcat
     3121 aatttcaata ataaaaaaga tacttatatc tatttttata tagtatctta tttgatattt
     3181 acatttattt tttatgtata tttttatatt ttaataattt agtttaacta tttttctatg
     3241 ctatttatat agaatatttc taataactca taaattctaa tcttttaata tctatccctc
     3301 aattaatgga tatgaattta tttaatttaa atacttgata tttttcatat ttatataaaa
     3361 atattacatt ttaaatacaa gttaataaaa atggaagaat atatgttaat gtaaatatta
     3421 agaataatac taaaaaatat tactggaatc tcaaattttt aataatattc cagtaatatt
     3481 taaataatat taaattaaga aaaatatttt cttatactta c
//
LOCUS       DAELSY010000249         3399 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.251, whole genome shotgun sequence.
ACCESSION   DAELSY010000249 DAELSY010000000
VERSION     DAELSY010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.251"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            484..3126
                     /gene="adhE"
                     /locus_tag="KPG09_003704"
                     /gene_synonym="adhC"
     CDS             484..3126
                     /gene="adhE"
                     /locus_tag="KPG09_003704"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG0968258.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPDRVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGNTDQEKVDNLIKAIDELKARVGLPKTIKEAGVEESKFLETLDVMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT         1320 a          425 c          639 g         1015 t
ORIGIN      
        1 aacttataga gattagaata attttagttt gtatattttt taaaaccata cagttgttat
       61 aaaaagaaaa attgcacaca agtcaaaaat aagtgttatt ttataaaaat acgacaaaaa
      121 ttttatttac tacttataca aataacatgc aaataacata taaaatttaa taagtaaaaa
      181 aaatgaaaga tataaattat cattttagta ttttatttag ttgaagtaaa ttattcaaat
      241 gaaatatatc tcatttagat aaaaaatttt agtaaaagta aaaaaaataa tatatttgtt
      301 aaaaaataaa ttgacaaaat attcagaaaa aaacttcttg acctaagaga gtaaaataaa
      361 taaaatctaa ggtatagaga tgaaatctat aaaattattt taagtataag ttaaaaatat
      421 agatggtgca tttaagattt ataaaaatta gagatgcata attattttag ggaggttttt
      481 agtatggaga aaaaagaaaa ggtagtagaa aaatcaaatg ttgaagtatg tagtcctgaa
      541 attgtcaaca gtgttgaaac attaagaatg agattagaag aaattagatt ggcacaaaaa
      601 gaatttgcaa catttacgca agaacaggta gataagattt tcttagctgc ttcaactgca
      661 gctaatcaac aaagaatacc tctagcaaaa atggcagtgg aagagactgg tatgggtatt
      721 gttgaagata aggttataaa aaaccatttt gcctcagaat atatctataa tgcttataaa
      781 gatactaaaa cttgtggtgt catagaaaaa gatgaagcat ttggttttac aagaattgct
      841 gagccagtag gtgttttatc agcagttata ccaacaacta atcctacatc aactgcaata
      901 ttcaaatccc taatagcact aaaaactaga aatggtataa ttttctctcc tcatccaaga
      961 gctaaaaact gtactataga agcagctaga gttgtacatg atgcggcagt taaggcagga
     1021 gcaccaaaag gacttatagg ttgggtagat gttccttcga ttgaattaac taatgttgtc
     1081 atggcagagg cagaccttat attagctact ggtggacctg gaatggttaa atctgcatac
     1141 tcttcaggta aaccagctgt tggagttggg cctggaaatg tacctgcaat aatagatgag
     1201 agtgcagata taaaaatggc tgtaagttca atcttagtat caaaatcatt cgataatggt
     1261 atgatatgtg cttccgagca agctgtaata gtaccagaaa aaatatatga agaagttaaa
     1321 aaagaattta aataccgtgg agcacatttc ctaaataaag aagaaactga aaaagtagga
     1381 aaagtagtaa taattgatgg ttcattaaat gccagaatag ttggacaacc agcacatgta
     1441 atagccaaaa tggcggatgt agaagttcca aaaacagcta gaataataat tggtgaagta
     1501 gaatctgttg aattaaatga accttttgca catgaaaaac tttctcctgt acttgcaatg
     1561 tacaaatcta aaagttttga agatgcagtt gctaaagctg aaaaattagt tgcagatggt
     1621 ggatatggcc atacatcttc gttatatgca gactctataa atcatccaga tagagtagag
     1681 aagtttgtaa atgctatgaa aacttgtaga gtattagtta atactccatc gtcacaaggt
     1741 ggtataggtg acttatataa ctttaaatta gcaccatctc ttactcttgg ttgtggttct
     1801 tggggtggaa actcagtatc tgaaaacgtg ggagtgaaac atttacttaa tatcaagaca
     1861 gtagctgaaa ggagagagaa tatgctttgg tttagagcac cagaaaaggt ttatttcaaa
     1921 aaaggttgtt taggtgtagc agcaagagaa tttaaagatg ttatggataa aaagaaagcc
     1981 tttatagtaa cagattcttt cctttacaat aatggataca ctaagaagct aactgatttg
     2041 ttggatgaga tgggaattaa gcatactaca ttctttgatg ttgctccaga cccaacatta
     2101 gcttgtgcaa gagaaggagc taaggctatg gctgatttcc aaccagattt aataatagct
     2161 gtaggtggag gtagcgcaat ggatgctggt aagataatgt gggtaatgta tgagcatcca
     2221 gaggtagact tccaagattt ggctatgaga tttatggata taagaaaaag agtatatgta
     2281 tttcctaaga tgggtgaaaa agcgtatttt gctgctatcc caacttcagc aggaactggt
     2341 tcagaagtaa ctccatttgc agttataact gaccaagatt caggtgtaaa gtacccatta
     2401 gcagattatg aattaatgcc aaacatggct ataattgatg ctgatatgat gatggagatg
     2461 ccaccaagat taacagcagc atcaggtgtt gatgctttaa cacatgcatt agaagcatat
     2521 gtatctatgt taagaactga accagctgac ggtctagcac tacaagcagg taaaataata
     2581 tttgaatatc tacctcgtgc atacaaaaat ggaaagaatg ataaagaagc aagagaaaaa
     2641 atggcaatgg catctactat ggcaggaatg tcttttgcaa atgccttctt aggaatatgt
     2701 cactctttag cacataaatt aggtgcattc caccatgttc aacacggtgt tgcaaatgca
     2761 ttattaataa atgaagtaat taagttcaat tgtgcagaag ctccaaataa gatgggtgca
     2821 ttctcacaat acagatatcc tgattgtata caaagatatg ctgaatttgc ttcttttgca
     2881 ggaatcaagg gaaatacaga ccaagaaaaa gtagataacc ttataaaagc aatagatgaa
     2941 ttaaaggcaa gagtaggact tcctaaaact ataaaagagg ctggagtaga agaatctaaa
     3001 ttcttagaaa cattggatgt tatggttgaa caagcttttg atgaccaatg tacaggtgct
     3061 aatcctagat acccattgat gagtgaatta aaagagatat atctaaaagt ttattatggt
     3121 aaataatatt cattgtacaa cataggttct attgggtttg tatataaatt attagatttg
     3181 aatataaata gagttttatt agagaaacgt agaagtaact ataaataata gattataact
     3241 taaattctga atggaaatga taaaaagtca gtaatttaat ggtaatttcg aatatgtgta
     3301 tttaatcaat taactttaaa attagtcgga aaattttttt attacatata attttaaagc
     3361 ataatatata aaaaacttta tgaaaaaatt ataaaaata
//
LOCUS       DAELSY010000250         3342 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.252, whole genome shotgun sequence.
ACCESSION   DAELSY010000250 DAELSY010000000
VERSION     DAELSY010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.252"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            217..864
                     /locus_tag="KPG09_003705"
     CDS             217..864
                     /locus_tag="KPG09_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG0968259.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTAFKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(1031..1600)
                     /locus_tag="KPG09_003706"
     CDS             complement(1031..1600)
                     /locus_tag="KPG09_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968260.1"
                     /translation="MKKFILCIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(1605..2504)
                     /locus_tag="KPG09_003707"
     CDS             complement(1605..2504)
                     /locus_tag="KPG09_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0968261.1"
                     /translation="MKLLSKVNNNIRLIIQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(2515..2664)
                     /locus_tag="KPG09_003708"
     CDS             complement(2515..2664)
                     /locus_tag="KPG09_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968262.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(2657..3241)
                     /locus_tag="KPG09_003709"
     CDS             complement(2657..3241)
                     /locus_tag="KPG09_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBG0968263.1"
                     /translation="MKKFFMVVLGCIFVLGVISGCSKKESKPVEKNKSNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKNIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEEWKKIIDERLKMVKANE"
BASE COUNT         1171 a          567 c          372 g         1232 t
ORIGIN      
        1 tatgttttat aagtatttct ttattcagtt atttgttaaa aactaatagt aaatcttata
       61 ctaatcgcat atttatgtta taactatatt gtaactattt tcgtaatctt aggaaaagat
      121 tacttataaa attaacctta tacaataata tatcgtataa tcttaattag cataatagtt
      181 acaatattaa aatttaaata tgggagtgat tttagtatga taagacaaac accattagag
      241 aaacgatatg acaagctagg tcctcatatt gtaacagcat tcaaaaaacg ttattttgat
      301 gcttattact gtaaaactag aaatgaagct ttgcaacaaa ttttagattt aattcctcaa
      361 aatcatgtag tatcatgggg aggatctgaa acacttaaag aaatgggcgt acaaactgct
      421 gttagagaga aaggatttaa agttattgat agagatttag caaaaactcc agaagaaaga
      481 actgaaatta tgcgtcaagc acttctatgc gatacttttt tgatgagtag caatgcaatt
      541 agtgaagatg gacaactctt taatattgat ggaaatggca atcgtgtagc tgcaatgata
      601 tttggtccaa aaaatgttat agttgtagct ggtatgaata aaattgtaaa aaacattgat
      661 gatgcaatac aaagaactag aacaattgca tctccagcag ttgttcagcg ttttcaagat
      721 gtcaatacac catgctatat taatggaagt tgtatagatt gcacaagtcc tgaaagcatt
      781 tgtgcataca tggttactac aagaatcagt cgtccagcaa aaaaaataaa ggtaatattg
      841 gtaggagaaa atcttggtct ataaaatata catacatgta aatagctcta taaataataa
      901 aatcatatga aataacttat ttctaaaagt ggtagaataa aagcttctac aagactttca
      961 ttctaccact tttatattct tttaatttgt gtttctatat tattaattgc tccaattttc
     1021 atatatatta ctaactattt ttattttcat aatttttttg ttcttcatca agctgtttat
     1081 aataatcttt attatagtca gctagataat ctataccacc tcctgtaatc acatttaata
     1141 atttcattgc agattcttga ttaactttac aaacataatt atcaccagat ttaacaagct
     1201 ctaatgtaat attaaattct tttttctttg cagaatcaat atctttcata gttggatttg
     1261 gcatcatttc tcctagttta ggaggattct tttttaaaat agaaaccata tcagtattgt
     1321 taatttttaa atctacagtc actttatcct tattattctt tatatctgaa atttcccaag
     1381 tcatattttt agaatacatt ttaaatcctt ctttatcttc gtatataaga ttacctaacc
     1441 ctgattttgg gaaatactta tcaacagtat ctgcatcttg tgtttttatt gaattaaaca
     1501 tactatccac ttcatttttt atcttattat cattagtaca accagctaac accagtactg
     1561 atattgcaaa cattataata catagaataa atttcttcat taaattatat ccccctatac
     1621 ttttttgcac aaatattatt ctctttttta tcttttgaaa aagttgtagt aattgcattt
     1681 tgtggacaaa cttttataca ttcaccacac ctaatacagt ctggactatt tacatcttta
     1741 tatattggta tatcaagctt acattttgtt tgacatgctg tacaatttgt acatttatct
     1801 ttatcaattt taaatctata tattgaaata ggattaaaaa aaccatagat tgctcctaaa
     1861 gggcatatat acttgcaaaa tggcctataa gtaatgattg acatcaataa tatcaatagc
     1921 aaaatactta acttccagct aaataaaaat cctattgatt ctcttaaatt ctcatttcct
     1981 aaaataagtg gtattcctcc caaaagtgtt cctgaagggc aaatccattt acaaaaccaa
     2041 ggatttccac cccctgctac atttacaata gcaactggca aaataattac aaacattatt
     2101 aagactatat actttagata ttttagatat ttatcaccat aaactttttt tatctttact
     2161 ggaaagggta ctttatgtag taaatcttgt acaagtccaa agggacaaag ccaaccacaa
     2221 acaaatctgc caaaaaaagc accaaatgcc attaaaaaac caacaacata aaatgagaat
     2281 ttatactctc tgcttgataa aactgcttgt aatgaaccta ttggacatgc tccaagtgca
     2341 cctggacatg aataacaatt caatccaggt acacataatt ttttactttc tcctccatat
     2401 atagttcctt ctaaaaatcc atttacatat ccatttgtta atgctgtaaa tgcaacttga
     2461 ataataagtc ttatattatt atttacctta gataataact tcattcctct tctcctaacc
     2521 aataccaata cactcaagac aaatcataat tgctttagtt aatactgtat ttgtttctcc
     2581 acgatatgta cctaatgcaa taaatataat actaattgca ataatagaaa tgcctttttt
     2641 attatcttta ataaagttac tcattggctt ttaccatttt tagtctttca tctataattt
     2701 ttttccactc ttccttacta tttgcaccta caaccttctc acccacaaag tttcctttct
     2761 tgtctacaaa ataagttgtt ggaaactcca taactctttt aaaatcagtt tttttaagtt
     2821 cttcagatac agtcaattgt tggtaagttg catctgactt tttcataatg tttttaacta
     2881 aatttttctc ttcatttgaa agaccagttc tcatatcagt tttatcaact tctacaacta
     2941 atccctttat agctacattg gaattattac tctcatattc ttttgataat gcttcaagct
     3001 ctggcatttc acccacacat ggtccacacc aagtagtcca gacattaata agagtaagtt
     3061 ctttatttga aaatatctct tttgttacct tatttccatc aacatcctcc gtatctaaat
     3121 tactaaacca ttcactatta ctcttgtttt tttctactgg cttagattct tttttgctac
     3181 atccagaaat cacacctaaa acaaaaatac atcctaaaac taccataaaa aactttttca
     3241 tatctcacac tcctagaatt tatatagtat ataaaatggc tgcttgaaat aaaaataact
     3301 tctcttctat attaaaacat tatatttaat aaataagaac ta
//
LOCUS       DAELSY010000251         3290 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.253, whole genome shotgun sequence.
ACCESSION   DAELSY010000251 DAELSY010000000
VERSION     DAELSY010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.253"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     repeat_region   1..946
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1..24
                     /rpt_unit_seq="cataccacatagttaatataaaac"
     gene            1593..2864
                     /locus_tag="KPG09_003710"
     CDS             1593..2864
                     /locus_tag="KPG09_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0968264.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSIADTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPM
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT         1297 a          448 c          407 g         1138 t
ORIGIN      
        1 cataccacat agttaatata aaactatgta agtcatttag tgttgcaggg tctggtaaga
       61 tttacatacc acatagttaa tataaaactc tattacaaca atagtaaagt ctataacaag
      121 attatattta cataccacat agttaatata aaacgaaaag aagacctaga taaaagaata
      181 tgcgatatag atttacatac cacatagtta atataaaact atgcttatat attatgttta
      241 ttctgttgtt tctaaaattt acataccaca tagttaatat aaaacgattt tttcatgcgg
      301 aggaaaattg atgtggggac atatttacat accacatagt taatataaaa cagataatct
      361 aggcacacat acaatgcgca aaactttatt tacataccac atagttaata taaaacattc
      421 agacgatata ggcgcaggag ctaagtattc tgaaatttac ataccacata gttaatataa
      481 aaccagcacg ccaagtataa gtaaaacttc cactatcacc atttacatac cacatagtta
      541 atataaaact aaatgttgct ccgtaaccta aaaacataat gctccaattt acataccaca
      601 tagttaatat aaaacgtgaa agaaaaatta atatgctatt tacattttac aaatttacat
      661 accacatagt taatataaaa ccattccaca atttatacaa ttttttctgt tctaaaacat
      721 ttacatacca catagttaat ataaaactct tcttatcttc cagttgcaaa atctatgctt
      781 gacctattta cataccacat agttaatata aaactagttt gagtggtaaa agtacgaagt
      841 acccctcttt aatttacata ccacatagtt aatataaaac atatgtaatt caccgtttta
      901 aattatagat aaattaaatt tacataccac atagttaata taaaacccca aaataaacta
      961 agtattttca gtacttacac acatacaact ctctcaaatt tgcagtgaac catgagtagt
     1021 gcaattgata acatttatca tacaccctca atcccttaca ttgcaactgt taaaccctac
     1081 tttatcaaaa atatcgctca ctgcaaaatt tatatatttt tattatatca taaaattcaa
     1141 actataaaat taaaatggaa taatttatca atttaagaac acctacatat ttgtaaatac
     1201 ttactatatt atctattcta ataattcttc ttctagctgg aactaatata aaaatcatct
     1261 ctgaaagatt aggacatact aatataaaaa taactatgaa tagatatttt cttgttttaa
     1321 acgatatgga taaaaaagct tttgaaaata taatcaaagt tctctttaaa taacattcca
     1381 ttttatgtaa gtaagcatta tatttttctt aaatgtcact taagtgtcag ttatatataa
     1441 taatagtaaa caactgtatt tcactaatat ttgctattat ctaatagtat ttatagttta
     1501 gatttgatat aatacatgtg gtaaaatttt gatttgtaaa acaaaatttt acctacatat
     1561 attaaaacaa atttaaaact aaggagacct atatgttaaa agtattaaaa ggaaatataa
     1621 tttttacaaa aacttcagaa actttcacag tatttgaaaa tagttatatt atactggaaa
     1681 aaggtaaagt aaagggaata tttaaagaga tacctaagga atacaaaaac ctaaaagtag
     1741 tagattacac tgataagctt ataatacctg gtatgaatga cctccatgcc catgcctctc
     1801 aatttaaaaa tcttggaatg gctatggata aagagctttt gccatggctt gaaacttata
     1861 catttccaga agagtcaaat tataaagata tagaatatgc aacaaaaatg tataaaaaat
     1921 ttgtaaaaga actttggaaa tctggaacta caagaatagc agtgtttgca acagtacata
     1981 aagaagcctc tatgaaactt atggatatat tcaaagaagt tggtcttgga gcatacgttg
     2041 gtaaagtaaa tatgaatatg aattgccctg atactttatt agaaaacact caggattcta
     2101 ttgctgatac agaagaaatt ataaataagt atagtgatac agattctatt gtaaaaccaa
     2161 taataacacc tagatttata ccaagctgta caagtgaact tttaaaaggt ttaggagatt
     2221 tatgtttaaa atataatatt cctatgcaat cacacttatc tgaaaattat gatgagattg
     2281 aatgggttag aagtctagaa cctgaatcta aattctatgg agatgcatat aatagatata
     2341 atttatttgg tcaaactcct actttaatgg ctcattgtgt tcattgttct caggatgaaa
     2401 tagatttaat gagagaaaac aatgttatgg cagttcactg tccagcttct aactttaatg
     2461 ttggtagtgg tgctatgcct atcagaaaat taatcgataa taatattaga ttagcacttg
     2521 gatcagatat aagcggtgga catacattgt caatatttaa agcaatggta tctgcaatac
     2581 aattatctaa attatattgg gttaattctg gtaagaagta taatttctta tctttatctg
     2641 aggcttttta tattgcaacc aaaagtggtg gaagtttctt tggaaaagtt ggtagctttg
     2701 aagaaggtta tgattttgat gcattgatta ttgatgattc aaatttaaat catgacaatt
     2761 attctatact tgaaagactt gaaagattta tctatgttgg tgatgataga aatatcattc
     2821 atagatatgt atgtggaaaa ctaattgaag aacctaatat ataattataa taaaaatttt
     2881 aattatatat tatggttaat ttatgcaact ataattaata aaagagtata ttttaaatct
     2941 aaaagctata ggttgaaaca tactcttttt attttgttta tttatagctt tagttattaa
     3001 tcatatataa atctacaaaa cgtattccta gtaacttata ttttttgata tctacaatca
     3061 taagactata ttattggatt catacctcaa aaatattgcc ttctgaacat tattatattc
     3121 aaagttatgt taatttacaa tgatatatct aacatgtatt gattaaaaaa aattaaaact
     3181 tgcttttcat ctcctatatc taaagaagtg gcatttagaa taaactattg taatattaaa
     3241 gttgaaattt tctagtttat ttactctata gcaccatatt ttaatactat
//
LOCUS       DAELSY010000252         3288 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.254, whole genome shotgun sequence.
ACCESSION   DAELSY010000252 DAELSY010000000
VERSION     DAELSY010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.254"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..510)
                     /locus_tag="KPG09_003711"
     CDS             complement(<1..510)
                     /locus_tag="KPG09_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0968265.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMIN"
     gene            complement(541..2775)
                     /locus_tag="KPG09_003712"
     CDS             complement(541..2775)
                     /locus_tag="KPG09_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0968266.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQYIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESSRELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLILFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
BASE COUNT         1150 a          430 c          374 g         1334 t
ORIGIN      
        1 atttatcatt ttgaaattat ctaagtcaat tagtacaata taatctccta taaatatgtc
       61 aaatctatca tttaaatatt ttctattata tgctccagtt aatgggtcag taattgagta
      121 gtgaagattt ctcttatttt cttcatagag ttttgttata tcattggcca tagtaattat
      181 gtaggttttt tcatcaacat ctactttgta ttctaaaaca ttccaatagc ttgtagtatc
      241 ttttttaaac tctaaaatcc aatttgaaat tttatcataa ggataatttt tttcccaatc
      301 taattgaata ttgtgagaat ccgtaaaaaa tttagcaata ttatcaggga gtttcttagc
      361 tatttctata ttagtcattc caagtggatt atcttgcata taacttttaa ttaaattttt
      421 taaattatca tttataaata taattctgta gttagaatct cttataatta gaaatcctgg
      481 aaaagttctt aaaaaaattt ctttaaacat attaatttct ccaaaataaa atactttgta
      541 ctagattttt gttaaatttt ttattttatt tacatctaca tacatacctt gggcaatcga
      601 ccttccatca attgcagaaa ggttacattg tgtatacata actgtgacaa tttgaccatc
      661 tcgttttatg aatctatata aaaatggttc aattttattt gtgtcaatca tatccaaact
      721 ttttttataa acaaagtcta aatcatctgg atgaacaatt gctcttaaat ttccgcccat
      781 ctcaccaaaa aactgttctt ttgtataacc tataatgtca aacatttctt cattcatata
      841 agtaataatc atatcaccaa ataaatcgta gcgaattata cctacaggca acattgaata
      901 aaattgtgat aaaatattac tttcatcaag atgtgtattt gtcattttag gtattgaata
      961 tgattcaaat tcatttatat agccagcagt tttaattaaa tccaaatgta attttgctaa
     1021 gagtaattta ttttctgaaa ttatacaatt tgcactaaag tagaaattgc tatatttttg
     1081 atgttcagaa gcttcatctg ataaaacggc ttctccacta attaataatg aatctgattc
     1141 agtattatgt ggggttatac ttttgtaaag aatagaaaat tttttacctt taatatgttt
     1201 ttcaaaatgt tctattactt taggaaaaga tgtaatttca gttttgttgt aaaaatcaaa
     1261 ccattgaatg tcttcagtga cattagctct tagttttttc atatcttgca taataaaaaa
     1321 tccatcaaat atcttctcta ttaaagattt tcctatatca gaatatttta taaacatttt
     1381 ctctgaatta ttctctttag atagtaattt ttcaaattct ttgcactcca tgggttttgc
     1441 ataataataa ccttgggcga tatcacaatt tatactttta ataaattcta catgttgttc
     1501 tgtttcaaca ccttcacata ctgtaagcat cccaagagct cttgcaatgt ttattataga
     1561 gtacaaaatg gttgtatttt taaaatcatt taaattatga gataaaaata tcctatcaat
     1621 tttgattata tcaaaaggta gattttgcaa cataccaaaa gatgaatatc cagaaccaaa
     1681 atcgtccatt gatacagaaa aaccataaga acgaagttca gctagttgtt gaataattgt
     1741 atttgtattt tctattaata tattttctgt tatttcaatg ctaaaatttg atgtagagac
     1801 gctgtacttt tcagtaatac cttttagcat acttggaaaa ttagggttaa aaaaatgaag
     1861 acgagaaaag ttacaacaaa ttggaacaaa aacatcattg ttatcaatcc atcttcttat
     1921 atttttacag ttctcttcaa ataaatgcaa atctaattcc gttatcagac caaactgttc
     1981 aaataaagga ataaatttat ctggagtaag aagtccttta atagggtgtc tccatcttgc
     2041 aagtgcctca catccaacta tagtattatt ttttatatta taaagtggtt ggtaaaatac
     2101 ttcaatttgt ttagtttgta gaacatgtga tatttctgat aagaactcct tatcattttc
     2161 taatttattg taaaaactat tatctttaaa atcaaataga atgtatctat ttttgccttc
     2221 acttttagcc ttttttaaag cactatatac ttggttgaaa agtatcaaag tagtcatgtt
     2281 tggttcacca ttccagatag tgctgccgat actaaatgta acattggagt atatttcttt
     2341 ttcattagga agtcttattt ttaattctcg ggagctctct ataatagttg cacccatatt
     2401 atttaactct ttatttgata catttggaag tacaattaaa aaaccttctc cttttaaata
     2461 agcgactatt ccctttgatt ctggaataaa gtgcaatagg ttttgagtta gagtaattaa
     2521 taaattttct ccgactagat atccatatat atcattaaat aatttaaagt tatcgatatt
     2581 taataacatg attcctatat gtatattttt cgaaatacag tcatcaatat attggtacat
     2641 aaattctctg tttggtattt tagaaaaaaa atctatttca tttaaggttt ttaatttaaa
     2701 atttctacta tctaattcct tagataatac ttctaccttt ttatttaaca tttttatata
     2761 attatgattt tgcatggtat tcctccataa gatactttaa attttgatga caactgtatt
     2821 tatgctagaa gtatttaaat gttaatttgt aaataattta aatgctaaat tttaaataag
     2881 aatttaaaat gaatatacat taagttattt gctccaatgc tacaaataca aatatttttt
     2941 agtttagtaa taatattaga taaaattagt taatgcttaa attatagtat attgaaagat
     3001 ttcctttttg aatgcaatta tatcatattt tttcataaat agatagattg tgagctgtat
     3061 agttagtaag gtagagtttt tttataatta attttaagtt taatgatttt tcaaaggcat
     3121 aattatttta ttcataatta actttttata cataatttaa taccacataa ttaattttag
     3181 ttattaaaat taagtttatt cttgtatttt ttgttaaaga ctataataaa tattcattta
     3241 ctaaatttat aaactattaa tctatagaaa tcagttttaa tatgaaaa
//
LOCUS       DAELSY010000253         3242 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.255, whole genome shotgun sequence.
ACCESSION   DAELSY010000253 DAELSY010000000
VERSION     DAELSY010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.255"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            434..1087
                     /locus_tag="KPG09_003713"
     CDS             434..1087
                     /locus_tag="KPG09_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG0968267.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIHGIIDGMKF"
     gene            1163..1372
                     /locus_tag="KPG09_003714"
     CDS             1163..1372
                     /locus_tag="KPG09_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968268.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(1512..2078)
                     /locus_tag="KPG09_003715"
     CDS             complement(1512..2078)
                     /locus_tag="KPG09_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBG0968269.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSP
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            2239..2892
                     /locus_tag="KPG09_003716"
     CDS             2239..2892
                     /locus_tag="KPG09_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBG0968270.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(3050..3226)
                     /locus_tag="KPG09_003717"
     CDS             complement(3050..3226)
                     /locus_tag="KPG09_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968271.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         1233 a          334 c          466 g         1209 t
ORIGIN      
        1 tcattagcat tagcaataat ttaacaactt aagcaatatc aattttattt attgattatt
       61 tttgaactga aaaatggggt tatatggtat actacaataa taattaattt tggatgagaa
      121 tatataacgt tacagaatat cttaggagta tatgtataaa aaatataata aaagtgtgag
      181 gtttagtaat taacaaatta tgtggcttca cactttttta taaaataaaa atacttagca
      241 gaagaatatt ttagttgata tgtttataag atagtacttg tttgggtaaa agtataaata
      301 gtaaaaaagt agaagtaatg tgtataaaaa cataagttac attctgtaat aatagttatt
      361 ttataattca acaaaagaat agaatgggta tatttgtgaa ataataatat aaaataatct
      421 taggagtgat cttatgaaga aaaaattatt atatataaat gtaaattcaa aacctgaaga
      481 cttatcttca agtaaaactg tagcaagaaa atttataaat aaatttatgg aaaaaaacaa
      541 agattttgag gttgaagaga tagaccttta taaagaacat ataccgagac ttgagtatca
      601 gtactttgaa aaaagaaata gcatagttag tgaagaaaat gctaaaaatt tagacgataa
      661 ggatagaaaa gagcttacaa agattagaaa tctttgtgac caatttgtga gtgcaagtgt
      721 ttatgtaata gcagcaccta tgtggagttt atcttttcca gcaccactta aagagtatat
      781 agattgtata attcaagatg gaaaaactat atcttttgaa ggcaatgata aaccacaagg
      841 aattttaaat gatattgata gaagtatggt atatatacaa tcttctggtg gacacatacc
      901 atgggtcttg aagccagtta tgaataaagg gttaaattat gtggaaagta ttatgaaatt
      961 tataggaatt aaaaaatttg atgaattgtt agtagatgga actggaacta gtgaagaaga
     1021 aagacaagct gcaatagaaa aagcatctga aaaaatacat ggtataatag atggtatgaa
     1081 attttaatta taatatttta ttaaaaatat aaaggaaaaa cctcttttaa tgtagaaact
     1141 tatattgaag gaggcgagaa tcatgcgaga agaaaaaagt aatgaaaagt atgattgtta
     1201 ttggtgtaat caagaaaata atttctgtgt agaaataaaa gataatatag taatgataga
     1261 tgatggcact ggtacgttaa aacaagccgt tttcataggg tataaacaaa tccaaattaa
     1321 tttaaattgt tcacattgtc aaaacttaaa tagaataaaa ttaaatttgt agaattttaa
     1381 aatggaaatt attgaaggaa ttaaaaatag aaagtgttaa tatttagact gaaaattata
     1441 ttttatacaa taaaagttta tttaaataac attgtatttt aatatttatt ttcagtctta
     1501 ctttattaaa attatttttt ggttatgtct ggaaaatatc tgtctactaa tttttctgaa
     1561 atatttttta attggtcagc cattgaagtc agacttaata aggctatcaa taaatcatct
     1621 ttattttctg gatttatact aatattttca aaattagact ctataaagtc cggtatattc
     1681 tttctttgat tttcttttat taacatgaat ttttcgtaac atgaaactaa atctttttca
     1741 ctgaaattat ccttttcata cacaccatgt aagattcttt taatttcttg tattgtaagt
     1801 gtatttttca tagaataaat aattatcatc tgtaaaattt gttctttagt atactttttt
     1861 cccttaactg gtttgattaa tccctctttg ctgtaattat ttatcatggt tttagtaaga
     1921 attttatcac tttcaaatct tttattagga gaaagtttat tgtcaattag agaaataatt
     1981 tgatccatat ataagtcaat agagggaata tcgtttgaag ttatatctgc attatttagt
     2041 gtttctagta ttatagtatt aagttcatta ttcatcatat gttacctcca taatatatat
     2101 aatacactaa attagaaagt aaaacaatat taagacttgt aaaaaaaaca taaagtggta
     2161 taattaaata tataatatag taatgaaaac tatatttatg aatctgattt ttaaaataca
     2221 taattgtagg aggacgaaat gtatcaattt tttttaaagg gtagagaccc aataagcagt
     2281 ttaactcatt ttataggtgc ttgtttatct cttttagcaa ctataatttt agtttttcaa
     2341 tctgttacat tacaggaaac ttctctcttg atgatagtat ctgtatcggt atttggactt
     2401 tcactaattg ctttatatag tgcaagttcg tattatcatt ttttaaaggg gacaccagaa
     2461 caagaattgt tttttagaaa agtagaccat gcaatgattt atgtattgat tgctggctct
     2521 tatacaccaa tatgtctgaa ttttatggaa aaaaaagaag gcataatatt tgttacagca
     2581 atatggatag ttgcatttat tggcataata ataaaaatat tttggatgga tgcacctaga
     2641 tggttatcta ccagtatata tttacttatg gggtgggcaa ttgtttttga tataaatgca
     2701 ttcaattcaa ttcctaagga ttgccttaga ctattaatta tggaaggtgt atcttactct
     2761 ataggtgcaa ttatttatat aattaaaaag cctaatataa gtcctgagtt tggatttcat
     2821 gaaatatttc atatttttat tatgataggt tctttatttc actttttagc agttttattg
     2881 tatgttttat agttttaaaa gatttgatta attgatacta aaattctaca atgaataaaa
     2941 tggaactgcc ttatatagat tatatcttcg acaataagac agttccttat tataaatatt
     3001 aaattctact tataataaaa ttttgataaa aatagtccat tcaaaatgcc tattccataa
     3061 ttcctttaat gtacttttca tttacgacat atagtttgaa tttatgtact ataattatat
     3121 cctctaaatt ctctgaaaca ctaacaattc tgacacattt gtttctaatt tctacaggta
     3181 aatctaaatc atttgttaaa ttcacatagt catcatattt atacataaaa tcatcccttt
     3241 ac
//
LOCUS       DAELSY010000254         3230 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.256, whole genome shotgun sequence.
ACCESSION   DAELSY010000254 DAELSY010000000
VERSION     DAELSY010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.256"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(50..157)
                     /locus_tag="KPG09_003718"
     CDS             complement(50..157)
                     /locus_tag="KPG09_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0968272.1"
                     /translation="MGRHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            complement(794..2374)
                     /locus_tag="KPG09_003719"
     CDS             complement(794..2374)
                     /locus_tag="KPG09_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBG0968273.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSTLIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
BASE COUNT         1041 a          576 c          322 g         1291 t
ORIGIN      
        1 tttatttgga tacattctaa acttataagc tttttccact actgccacct cactttactt
       61 gctttttctg attattaata tattttctta tctgctcctc tgtattttct gatacagtgg
      121 caacgaaata actaggattc caaagatgtc tccccataat actggatgtc tatatttgac
      181 atattatatg catatcctct accatgagta acttccattt ttcatcacct atcataatat
      241 taaaatatgc aattacttaa aacaagtatt ttgaaggttt tttatattta tttttaaata
      301 acatatttaa gaaccgtaag tcttaacttt tatcattttt gaggttgtcg ttcacataag
      361 ttcgctacta cctatgcagt tctcttatga acttcttaca ctttcatgta agcacagact
      421 atatcttatc cttcggcatt atccgctaag gtctatccac ttccactacc gataacttgt
      481 agtgtacttc cctcaagagg aatagtcgtt gaagtttctc ctattcggag cttacctgct
      541 gattgcccat tttaaagtac ttaggattta accttatacc atctaaccaa ttttttctac
      601 tttcgtaact ttcacactta ggtttatttc atccttatgt tttagtttag ttagctttag
      661 gggattttag caattcaagt agtattggat aggttctatc ctatctctac atataggttt
      721 ccctatatgc tgactatttt agctactaac tcatgactaa agtcacaagt gtgcgtagct
      781 attttttaat caattatata tccaacaact ctttcattgt taatgaaatg ttacttatta
      841 aatccgtagc atttaccgta tacattgctt tgctcaattt atctcctata tagccatgta
      901 tatatgctcc actaactgta gcttccaata tatccatacc ttgacctata aatgaagtaa
      961 tcattccaag caaacaatca cccattccac cagttgccat acagctattt cctgtaggat
     1021 ttatatatgt ttttttacca tctgttatga cagtttgata accttttaaa agcaccacca
     1081 gattatattt ttgagcaaat tcttttgcaa catctactct atgttctctt atatacccaa
     1141 ttgaatctcc tgtcaacctg gccatttctc ccaaatgagg tgtaattaca aatctatttt
     1201 tccattctaa aaacttataa catctgtcct tcataacgtt caatccatct gcatctaata
     1261 ctattgggca attagaattt tctactatct taagtaattt atcaaatgtt tgactattat
     1321 ctcccatacc tggaccaaaa ccaattgcat cactagaatt aagtaattta tttaatcttt
     1381 cttcttcaaa atttaaagtc attgcttctg taagtctaat agataccttt tcttgcacat
     1441 cttcactact aattaatgtt acaagtccac tcccactctt aacagcagat tcagttgcta
     1501 tgaaacttgc accataaaat ccttttgaac ctgcaactat agatactttt ccaaaatcac
     1561 ttttaaaacc atgcttatct cttcttctaa tattattttt tacaaaagtc ttatcagtta
     1621 tgaactcttc atcatggtat tttcttgtaa taaaatctgg aacacctatc ttttctacaa
     1681 ctacatcacc tataagtgtg ctagtgtcat agtttagaaa acctctttta taaaactcaa
     1741 agctaatcgt tttgtttgct ctaatacaag ttcctaatac atttccatta tcgctatcta
     1801 tacctgaagg aatatctata gaatatgtat ttttgctatt atcgtttact atgtcaataa
     1861 cttctttaaa aataccagtt atatcacgtt ttaatccagt cccaaatata gcatctacta
     1921 caacatcact tttagatact ctatctttaa aaattgttaa atttttattt tttataatat
     1981 tttgtttctc aactttaata attttatcta cttcattttc atctccataa tttttttcac
     2041 ttaaatcatt tttattaata tttataatat taattcccat atttaaaagt acattgtaat
     2101 ttattttaga gcattcactt agtttttcaa gattacctac taaaaatacg tttacttctt
     2161 ttccatatgt atttagatgt cttgctattc caagaccatc tcctccatta tttccaacac
     2221 cacatacaac tgtataacta ttatacatgt ctatatccat atttttcatt gcagatataa
     2281 ccgcattttc catcaataca atcattggaa tccctacttc ttctacacat gcagtatcaa
     2341 catttctgct taatttagat gtacctatta gcataaatta tcactcctat aaagttaaag
     2401 acttcttaaa tacttaccaa aaatatctat tgaaatcttt taatcccata aatatcataa
     2461 ataaaatcac taaaactaag agttttgaaa taatttaaaa taacaaaaaa ttttgaatta
     2521 aaacaattat ttaatttatt ataccatata atttaagtta ttatgttgat ttaaaaccta
     2581 tttacactaa ttatctaaat atctctttat ttttataatc ttttatgact tatttatatt
     2641 tttcattttt ataaaaaaac aataattaag aatataacta tattctacat gtaaaaattt
     2701 ttttatttaa actcaatttt ctgataactt gataattttt caaaatttat agttgacttt
     2761 tattttttac tgtattaaac taattactat acaaaacaga aaacaaaatt ttcaaaatta
     2821 ttaaattaaa aggagaggta aatatgaaga ctttattaaa caaacaagta cgatttttta
     2881 gtagccaatt tagttagggt ttgtacctaa gttaagacaa attgtcataa catagataca
     2941 agccccttag gattatgatt tttaaggatt gtatctatgt tggctatagt aattgttgtt
     3001 aaagttttta ttttatcatc ataatataga gccacatgga ttatactgtg tggcttattc
     3061 gaatttttgc catacagtta tatatactgt gtggtttttt tatatcaaaa tcatttttta
     3121 aaaccaaatt taaaaaacca tttaaaatat tttcaaaggg gtgttttatt tatgtcaggt
     3181 attatatctg taatgacgca aagtttaatt ttatcgatta tggcactggg
//
LOCUS       DAELSY010000255         3178 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.257, whole genome shotgun sequence.
ACCESSION   DAELSY010000255 DAELSY010000000
VERSION     DAELSY010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.257"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            82..276
                     /locus_tag="KPG09_003720"
     CDS             82..276
                     /locus_tag="KPG09_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HBG0968274.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIREFQDTIIL"
     gene            complement(378..887)
                     /gene="vanZ1"
                     /locus_tag="KPG09_003721"
     CDS             complement(378..887)
                     /gene="vanZ1"
                     /locus_tag="KPG09_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HBG0968275.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1207..1597)
                     /locus_tag="KPG09_003722"
                     /pseudo
     CDS             complement(1207..1597)
                     /locus_tag="KPG09_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            2041..2610
                     /locus_tag="KPG09_003723"
     CDS             2041..2610
                     /locus_tag="KPG09_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968276.1"
                     /translation="MKLRNPKIKKLLSVALLAALIGIPIGQTANAMEISNNVPSYIEN
                     NPTTIEDLEINDSQLPKPGTVTYIDINKLIKNLKENGIDTTLIENELYYRKAGTNKIV
                     WTKTGFDLYLSKGVALTLAGAGTGVAGLLAGAIPGIGQYLAAAIVGAITMHLGSNITS
                     GKIFKFKKMRYNKGGAYVTKYYLQSVRNQ"
     gene            2902..>3178
                     /locus_tag="KPG09_003724"
     CDS             2902..>3178
                     /locus_tag="KPG09_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968277.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYISIGLFTRRLDITIIAIILVATITV"
BASE COUNT         1184 a          408 c          375 g         1211 t
ORIGIN      
        1 tacagtttta acaatcatta ttttcagaaa agtgatacta ttgaatatat taagaataat
       61 tagagatgga ggaactaaga tatgagcgta attgtaatgt gtagtcgttg tggaaaagaa
      121 atagataaat atagaaataa atatataact tattatgaga aaaataccaa tgcagaaatt
      181 aaagtttgta taaattgtgc attagattta ttagaaaaaa caagagaaaa tcaagaattg
      241 aacatacgag agtttcaaga tacaataatt ctttaataat ttaggaaagc acctatatag
      301 gtgcttttta tgtgtaaata attttatatg taactgtatc ttacgttcta ttttaagcta
      361 aacctatata cttatttcta taagtttgca aaaataacta atcctaaaaa taatacaaca
      421 ataattgtca caatcaatgc caatacattg ataattttta tggtattatt cttaaatatt
      481 ttcgataata gcataaaaac agctattcct atgattcctc ctagtgtgtt tcctatcaaa
      541 tcggttatat cacttgcacc tagtgcaaat atatactgaa gtgtttcaaa tgctaaacta
      601 gttataaaaa taggtataac tttttttata aagctccatt cttcttttag catacacaca
      661 tatatcccaa atggtacaaa tatagccaca tttaatataa tctctgatat atctactcta
      721 ttattaatta taagtgaccc agcaaaaggt actaagttta tgcttctaag attcatggtt
      781 tcaagagatg agatatcaaa ttgcaactta aatagtatta tccatgttaa aataattatg
      841 taaactataa ataatccttt tgtaatatta tgttttctag atttcattta ttacctcctt
      901 actatattat tgtattattt tattaataca ataatatagt aaggtaattt atctgtcaac
      961 tatttcttct aaaattatat tccttttata atttcaatat ctttatcata tttacatgat
     1021 ttaagctatt gtagtatttc atatatcttt aaattttaat tggtattatt ctctatattt
     1081 tttactatca aacactaagg aaataatcac ttttctttat ataatgtttt accatcacat
     1141 aaaactaaca ttaaatccaa acgctactct caattgtctc aaactaaaaa gatactgata
     1201 gctttattag tcatcaatat cttcttcttt caaattttta aagtattctt ctaatttgtc
     1261 caagctctta ctatctaaaa cttcatcatc atgtagtgct gaaataaaac tcttaaatga
     1321 attgttatgc ataaaactaa aaaaactctt tgtctcaact tttaagtaat ctttctcttt
     1381 tatagctata gtataatgcg tacatctatc taccttaata agtagttaaa aatcccttat
     1441 ctactaaagt ttttaaaaat gtcaatgttg tgctcttttt ccaatcgtat ttcaacttca
     1501 ttgcttctat gatatctctt gaggctaata catcatctgc tccccaaata tacttcatta
     1561 gttttagttc tgcatgtggt aatttattaa tcttcattag tttacacctc ttaccaattt
     1621 atgtataatt aaattttata ctaataatag tatgtaatca atcagattgg aacaaatata
     1681 atattccaat ttattccata taaaaataat taataaaaaa gaagtactct actttagaga
     1741 gtacttttgg attatatatt caaaaattta tctaacatga ttagagtatg ttttatgctt
     1801 tagtgtgata attttagttc gttttattat tataatatgt attatatttc attataatct
     1861 ataagcttaa atacaggata tttatttcat atacttaatt tatattaagt tttattatat
     1921 gtattcttaa gttttactat attttttatt aatatttaat gtagttaatt cttaagaatt
     1981 atattctaca ataataatat atatctttga tatataacta atttaaaatg gaggtatttt
     2041 atgaaactaa gaaatccaaa gattaagaaa ttgttatcag tagcattatt agcagctctt
     2101 ataggtatac ctattggaca aacagccaat gcaatggaaa tttcaaataa cgtcccaagt
     2161 tatatagaaa acaatccaac aacaatagaa gacttggaaa tcaatgactc tcaattacct
     2221 aaaccaggta cagttacata catagatatt aataagctaa ttaaaaacct taaggaaaat
     2281 ggtatcgaca ctactcttat tgaaaatgaa ttatattata gaaaagctgg aacaaataaa
     2341 atagtttgga ctaagacagg ctttgattta tatctttcta aaggagttgc attaacttta
     2401 gctggtgctg gtacaggggt tgcagggctt ttggctggtg ccattcctgg tataggacag
     2461 tatcttgcag ctgctatagt tggagcaatt acaatgcatc ttggttcaaa tataacatct
     2521 ggaaaaatct ttaaatttaa aaaaatgcgt tataataaag gtggggctta tgtaaccaaa
     2581 tattacttac aatctgtaag aaatcaatag gtgataaaat gattataaaa tatctacttt
     2641 ttgcaattat ctcagcacta gttttattct tttgtaataa agtatttcaa ctaagtaaat
     2701 tacacaagtt cattatattg tttgtttcat tttttatact aacttttgta gcatcattta
     2761 ttaatagata aaaaataaga tggtggggtt aacctaccat ctttttctca tttaatattt
     2821 gtaatatttt agatactctt tatacttgtg ttactgatat actttgatta agaaaagtaa
     2881 atttaacagg aggtatctgc tatggatttt ggaaatatca atcttatact tattggaata
     2941 atagttataa taggaactac aataatatat ttaattaaac ctaaaactgc tttttgtagt
     3001 aaaaaatatt ttaataaatt agaatcaata tatggaaata tagataagaa aaaaactgtt
     3061 aaactagaag tactttatcg ctatgtaaca ggtcttgagt atatatcaat aggcttattt
     3121 acaagaagat tagatattac aataatagca ataatattag tagctactat aacagtaa
//
LOCUS       DAELSY010000256         3139 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.258, whole genome shotgun sequence.
ACCESSION   DAELSY010000256 DAELSY010000000
VERSION     DAELSY010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.258"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(323..1234)
                     /locus_tag="KPG09_003725"
     CDS             complement(323..1234)
                     /locus_tag="KPG09_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG0968278.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SVGFPLYSKVGAAGGYHVLKERILPPITFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENMRRKLVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSIRTIVPIGLYAMNGLWYCPAYCIKSESVKEFRADRIVKILSIENLSNKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKK"
     gene            1345..1812
                     /locus_tag="KPG09_003726"
     CDS             1345..1812
                     /locus_tag="KPG09_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968279.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIEESYNFAKQKFKKRKNK"
     gene            complement(1980..2579)
                     /locus_tag="KPG09_003727"
     CDS             complement(1980..2579)
                     /locus_tag="KPG09_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968280.1"
                     /translation="MKLKRTLFVGLALVLAIGITACARDNEKPNENAGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKRMNDDLIAEGERLVKDIDERIEKLDKVSDEDMKKGQN
                     DFMKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            2825..>3139
                     /locus_tag="KPG09_003728"
     CDS             2825..>3139
                     /locus_tag="KPG09_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBG0968281.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGY"
BASE COUNT         1071 a          480 c          378 g         1210 t
ORIGIN      
        1 ccacttccac taccgataac ttgtagtgta cttccctcaa gaggaatagt cgttgaagtt
       61 tctcctattc ggagcttacc tgctgattgc ccattttaaa gtacttagga tttaacctta
      121 taccatctaa ccaatttttt ctactttcgt aactttcaca cttaggttta tttcatcctt
      181 atgttttagt ttggttagct ttaggggttt ttagcaattc aagtagtatt ggataggtgt
      241 tatcctatct ctacatataa gtttccctat atgctgacta atttagctac taactcatga
      301 ctaaagtcac aagtgtgcgt agctattttt taatcaaatg tttaagttct ataggttcta
      361 ttattgtagc attttttcca aaagttaaaa aatattcagc cacccaatta agtgtagatt
      421 ttggtatata catatctatg tatccaccct tagacaaaat tttcaatcct cttgccaaca
      481 aaaattctgt ttcacaacgt tttactccct catctgtcag attaatttta atatgataat
      541 ctgtacctac ttccatattc ttaagataat catgtataga agataaaacc ttatatttct
      601 tatttgataa gttttctata ctcaaaatct tcacaattct gtctgcacga aattccttaa
      661 cagattctga ttttatacag taagctggac aataccaaag tccattcata gcatacaaac
      721 caataggaac aatggtacgt atactatttt tttgctttga tgaatattca attttaattg
      781 taaattcatt cattactata ttaaacaatt ccttcaataa tggtgttgag caatgcctat
      841 ctggtgtcca aaaaacaagt ttacgtctca tattctcaat attatattga atatcatttg
      901 gtatacaatt taaaaatttc tttaaaacag aaatagtttc ttgctcaaat ggtaaatcat
      961 tataatactc tagtgactga tatgcaaaga atatagattt tacctcactc tctgaaaatg
     1021 taattggtgg aagtatacgt tctttaagta catgatatcc tccagccgct ccaacttttg
     1081 aatatagtgg aaatccaaca ctttccaata cctgtaaatc tcttagtata gtacgttttg
     1141 atacagaaaa ttcttctgct agctctcccg ctgtaaaact tctttttgca tttattgtta
     1201 tcatcagttc aattaaacgc tcagctcttg acatattcat actcctaatt taaatatagt
     1261 gacaataatt gtcaccatta tatgataaga tattcttgca tctaaagcaa taataatatt
     1321 aaagaaagaa gggattgata ttttatggaa aatgatacta ttaaggctga tgatattctc
     1381 aattattgtc tatcaaactt agatgatgtt gtactaatgg atagttgggg ggaacgagca
     1441 atttactaca atcctaatgg tgttttaaag cgaggggtat atgttcttac cattaaagaa
     1501 aaagacagta atcatgataa aggttcgttg gttagtcgtc caaatgtata ccgtgtgaat
     1561 ataggattaa aaaaagaaac ttttattgaa atgtttggat atattccaaa gcgtccaggt
     1621 gtagggcaaa tagttgatat ggattttgat tttacaaaat tggacacaat catgccacat
     1681 cctatctact catggatggg atggatatgc gtcctaagcc ctactgaaaa gacatttgag
     1741 aactttaaaa ctttaataga agaatcttac aattttgcaa aacaaaaatt taaaaagaga
     1801 aaaaacaagt aaaaacataa aaaaataacc tctaaataaa ctataatgaa aatatgaggt
     1861 tacaagataa aaattagtat cttgataaca tacaaaaaag cgaagccaaa aaatatatta
     1921 attacaaggc ttcgctatat ttcaaatatt tatttctaac tttaaaacta tttactctat
     1981 tattttttat tatttcctgt tattcccaat ctatcattca tatcttttat catttttcta
     2041 aaaccactac caatatcgtt tccaacatct tcagttccat gaactagttt cataaaatca
     2101 ttttgacctt ttttcatatc ttcatcagat accttatcta atttttctat tctctcatct
     2161 atatctttta ctaatctttc cccttcagct attaggtcat cattcattct ctttaactct
     2221 gtatcttctg ttttaccgtc ttttttaaga gatgttacaa atgattgtat tttatctcta
     2281 ctatctttat atgcagcctt tacatcactt agatattttt catttcctgg atattcttta
     2341 tctttaaact gattgtttat actttctgga gtatcataaa tagaatatct atttagacct
     2401 gctatataat cattataact tgttgaataa taattcatat atccttcatt attatttaca
     2461 gattcattag gattttttcc tgcattttca tttggttttt cattatccct agcacatgca
     2521 gttattccta tagctaatac taatgctaat cctacaaata aagttctttt taatttcatt
     2581 tttattaccc tcctagatgt tttgtactac aaaaaatttt aataattttc tttcttaatt
     2641 tagtatttct atatttttat tttttattta ctaaaattaa acaagttata atttattttt
     2701 ttggaaatca taatgaataa ataattaaat aaaaaaatta taattttatt attgatattg
     2761 attatcattc atagtataat ctaattactt gatattaatt ttcatttgaa ggaggttatc
     2821 ttttatgtct tgttgtaatt cacaattttg ttgtaaaaat aaaattgaat catcatatat
     2881 aaaatggctt ttagaggttt taggtccagt ctttttaggc tctaaacctg ctgaaataat
     2941 aaatatttcg tttactgatg taaatagaga ggaaaaaatt aatgatattt ataaatatct
     3001 atataaatgt aagaaaatag attttatagt tattgataaa gaaaaaaagg gattaaaaat
     3061 cttatttgta aataaaaaag cattatcaga aaaattaaaa tgtaaaaaaa cagtaaactt
     3121 tttaaagttt ttaggatac
//
LOCUS       DAELSY010000257         3128 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.259, whole genome shotgun sequence.
ACCESSION   DAELSY010000257 DAELSY010000000
VERSION     DAELSY010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.259"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            168..1085
                     /gene="pfkB"
                     /locus_tag="KPG09_003729"
     CDS             168..1085
                     /gene="pfkB"
                     /locus_tag="KPG09_003729"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG0968282.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYAKRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            1122..3041
                     /locus_tag="KPG09_003730"
     CDS             1122..3041
                     /locus_tag="KPG09_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0968283.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT         1205 a          348 c          627 g          948 t
ORIGIN      
        1 gatgggtagt attgtatata cacctgtaaa tttggataat ataaaactaa ataagataaa
       61 aacgataaat ttctctttta attaagagaa attttttgac tttcaaggat attaatataa
      121 agttaagata taaacataaa gcaaataaat aaaaaagagg tggcaatatg atttatacag
      181 tgacattaaa tccatcaata gattatatag ttaagctaga tgagctaaag acaggaagta
      241 caaatcgtgt aaaagaagaa tacgtttatc caggaggaaa aggtataaat gtatctcgca
      301 tacttaagga acttggaaat gataatacat ctttaggttt tataagtggt tttactggag
      361 aatatataat aagaacattg gaagaaaaag aattgaagac agattttata aagataaaaa
      421 gtggtttttc aagaataaat gtaaaaataa aggcgttaaa ggaaactgag attaatggac
      481 aaggtccaaa tatagatgat gaagacattg atactttata taaaaaactt gataagctaa
      541 atcaagatga tattttaata ttggcaggaa gtataccatc tactttagat gaaaaactat
      601 acgaaaatat aatggctaga ttagaaaaaa agaatataaa agttgtagta gatgcaacta
      661 aaaatcttct tcttaatgtt ttaaaataca aaccttttct aattaagcct aataacgacg
      721 aattagaaga actatttggt gttaaattaa attcaattga agacatggta aaatatgcta
      781 aaagattaaa ggaaatggga gctataaatg tcttggtatc aatgggaaaa gatggagcat
      841 tgttgattac agaggaagat gaagtgctta taagtgatgt ccctaaagga aaagtaaaaa
      901 attctgtagg agcaggtgat tcaatggtag caggatttat atctgggtat ttgaatactg
      961 ggaaatatga ttatgctctt aaattgggag cagcttcagg tagtgcaact gctttttcat
     1021 atgacttagc caaaagagaa tacattgata aattggtaaa tgagatatct gtaaagcagt
     1081 tttaaaatat taaaaaatat atagaaaggt aattaatatt tatgaaaatt atagatttat
     1141 tagatgaaaa gtcaattaag ttaaatttaa attcaaaaac caaatcagaa gcaatagaag
     1201 aattagtaga tttggtcgca aatagtggaa atttaaatga taaagaaaac tacaaaaaag
     1261 caattttggc tcgtgaagaa atgagtacaa caggaattgg agaaggtgta gctatacctc
     1321 atgctaaaaa ttcttcagtt actaaagctt gtatagcagc agcagtgtca aaagaaggta
     1381 tagattatga atcgtttgac ggaagtttat caaatctatt ttttatgata gctgcaccag
     1441 acggagctaa caatactcat cttgaagtat tatcaagatt atctacaata cttatggatg
     1501 aagactttag aaataaactt ataaattcaa gctctgaaaa agaatttaag gaaataatag
     1561 ataaaaagga aagagaaaaa ttcagtgaag aatatcaaga tgagaaagtg gctgaaaaaa
     1621 atataataga tactaaagag aatgatgcta ataagtatcc taaggtttta gcagtaacag
     1681 catgtccaac tggaatagca catacattta tggcagctga aagtttaaat aaaatggcag
     1741 aaaacaaagg tgtatcaata aaagtagaaa ctaatggttc agcaggtgtt aaaaataagc
     1801 ttacaaaaga tgaaatagaa aatgcaacat gtataatagt ggcagcagat aaaaatgttg
     1861 agatggctag atttaatggc aaaaaagtca taaaaactaa ggtggctgat ggtatacaca
     1921 aagcggaaga acttatagac aaggctgtaa atggagatgc tccaatttac catggaggag
     1981 atggaagtca caacgaatca aatgaagaaa gtgaaagtgg atttagaaaa gtatacaaac
     2041 acttaatgaa tggtgtatct aatatgttac catttgttat tggtggaggg atacttatag
     2101 cgatagcctt tttacttgat gattatacta ttaacccttc aaactttggt tcaaatacac
     2161 cgatagcagc gttttttaaa gggataggag ataaagcttt tggatttatg ttacctgtac
     2221 ttgctggata tatagcatac tctatatctg atagaccagc gtttgttgta ggatttgtag
     2281 gaggtgctct agcaggtgat ggtggttcag gatttttagg tgcactactt gcaggtttta
     2341 tagcaggtta tttagtagaa gggcttaaga aaatatttag tgtactacca gcttcattgg
     2401 aaggaataaa accagtattg ttataccctt tacttggaac acttttgatg ggaattataa
     2461 tgacattttt agtaatacct ccagttacag caattaataa tgcaatggtt ggatttttaa
     2521 atggtttagg cggaacttca aaaatagttt taggattagt attaggtgga atgatggctg
     2581 tggatatggg tggacctgta aataaagcag catatgtatt tggagttgca tcacttgaat
     2641 cagggcagtt tgaaataatg gcagcagtaa tggctggtgg tatggttccg ccacttgcta
     2701 tagctcttgc aactacattt tttaagaata gatttactaa agaggaaaga gattctggta
     2761 aagttaatta tgtaatggga ttatcatttg ttactgaagg agctatacct tttgcagctg
     2821 gagacccact tcatgttata ccagcttgtg ttggaggttc agcagtggca ggagcactta
     2881 gtatgttgtt caacgcagct cttcgtgcac ctcatggagg agtatttgtt ataccagtgg
     2941 ttacccatcc atttgcatat atattagcta tagctgtagg tgctttggtt ggaatgatgc
     3001 ttttagcatt acttaaaaaa ccattaaatc aagaagtata gcaattaaag gatattcaag
     3061 aacttaaaat atgatgtttt ataaaaatta atatattgct gaattatttt aaaataagga
     3121 ttttatta
//
LOCUS       DAELSY010000258         3101 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.260, whole genome shotgun sequence.
ACCESSION   DAELSY010000258 DAELSY010000000
VERSION     DAELSY010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.260"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1090
                     /locus_tag="KPG09_003731"
     CDS             <1..1090
                     /locus_tag="KPG09_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0968284.1"
                     /translation="RIYPNKKQQELINKTFGCCRFVYNKYLAKRIDVYKSDKETFTYK
                     QCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKFKSKKINRFSY
                     KTNFTNGNIMYCGQHIKLPKLGMVKVRDKQIPQGRILNATISKEPSGRYYVSLCCTDV
                     DIEVFENTNNQIGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQRELSRKTIGSL
                     NRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLI
                     SDVSWSEFVRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPS
                     CNEIHDRDINASINILKEGLRLITIQNK"
     gene            1374..1820
                     /locus_tag="KPG09_003732"
     CDS             1374..1820
                     /locus_tag="KPG09_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBG0968285.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            1841..2884
                     /locus_tag="KPG09_003733"
     CDS             1841..2884
                     /locus_tag="KPG09_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBG0968286.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT         1294 a          326 c          551 g          930 t
ORIGIN      
        1 tagaatatat ccaaataaaa aacaacaaga gttaattaac aaaacttttg gatgttgtag
       61 atttgtatat aataaatatc ttgctaaaag aatagatgtt tataaaagcg ataaagaaac
      121 ttttacttat aaacagtgta gttctgattt gaccaattta aaaaaagaat taaagtggct
      181 taaggaacct gacaaatttt cactacaaaa cgccttaaaa gatttagata atgcatataa
      241 aaaatttttt aaagaaaaag caggatttcc taaatttaaa tcaaagaaaa ttaatagatt
      301 ttcatataaa actaatttta caaatggaaa cattatgtat tgtggtcaac atataaaact
      361 acctaaactt ggtatggtta aggtaaggga taaacaaata cctcaaggga gaatacttaa
      421 tgctactata tctaaagagc caagtggtag atattatgta tcattatgct gtactgatgt
      481 agatattgaa gtatttgaaa atactaacaa tcagataggt ttagatttag gaattaaaga
      541 attttgtatc tcaagttgtg gagaatttat agaaaatcct aagtatctta agaagtcatt
      601 aaacaaactt gctaaattac aaagagaatt atcgagaaaa acaatcggta gtttaaatag
      661 aaataaagca agattaaaag ttgcaagact tcaagaacat atagctaatc aaagaaatga
      721 ttttctacaa aaactatcta ctaaattaat aaaagagaac gatattattt gcatagaaga
      781 tttacaagta aagaatatga ttagaaatcg taaactatct cgcttaattt ctgatgtgtc
      841 atggtcagaa tttgttcgtc aattagaata caaggctaat tggcatggta gacaaattgt
      901 aaaggtaggt aaattctttg caagttcaca aatatgcaat aaatgtgggt ataaaaacga
      961 ggaagtgaag gacttaaata taagagaatg gatttgtcct agttgtaatg aaattcacga
     1021 tagagatata aatgcaagta taaatatatt aaaagaagga ttaagactaa taacgattca
     1081 aaataaataa cttatgtaag aaccgtagga actacgggga tagcctgtgg agaattagta
     1141 agacatatgt tagtatgcaa aattctatga agcaggaacc ctgtgacttt agtcatggga
     1201 ggttcagact actttgtaac aattttaaca aagtatgttg atataatact attaaattaa
     1261 tcttagatta gtttattcta ttgatttgta gtttaaattt atattcaaaa acattgtata
     1321 aacatataat ttatataggg ttttataatt catatgaaaa gagggtagat agtatgaagt
     1381 taatatggtt gatagtagca gtcttatttg gaatagcaga aatgttaaca ccaagcttaa
     1441 cattgatatg gtttagtgta ggagctgtaa tacttatatt tttaagtagc tttatagaga
     1501 gtattttctt acaaatttta atatttgcag taatttctat agcaatgtta gtcgtagcta
     1561 ctaaaaaaat agtaaaaaaa gataaaggct ataaatctaa cactaattta caagcaatga
     1621 tgtctaaaaa aggtattgtt acagaagaga tttctccaaa taacacaggt ctagttgtag
     1681 ttgaacatga aacatggacg gctatttcta tagatggtga aaagatagaa aaaggttcta
     1741 cagtagaagt attaaaaatt gaaggggtaa aattagttgt taaaaaagta gacgccacag
     1801 ttaatgtgac aaaccaatag aataaaggag gatttatagt atgggtacta aaattgtttt
     1861 aagtattgta ttaatagtag tagtcgtagc aataagttta acgtgtataa gagttataaa
     1921 acaatctaaa gtaggtataa taatgagact tggtaagttt caaaaagtgg ctgaaacagg
     1981 tgtacatttc ttaataccat ttttagataa aatggcatat gtaattgacc ttagagaaat
     2041 tgtaatagat tttccacctc aaccagttat aactaaagat aatgtaacta tgcagattga
     2101 tacagttgta tattataaag ttacagatcc agttagatat gtgtttgaga tagctaatcc
     2161 aatagctgct attgaaaatt taacagctac aacactaaga aatataattg gtgaacttga
     2221 tttagatgaa acattaacat caagagatat aataaatgta aaaatgagaa ctatccttga
     2281 tgaagcaaca gataaatggg gtataaaagt aaatagagta gagttaaaaa atataatgcc
     2341 tcctcaggat attcaagttg cgatggaaaa acaaatgaga gcagaaagag aaagaagaga
     2401 ggcaatactt caagcagaag gtaataagtc agctgcaata cttcaagcag aaggtgaaaa
     2461 acaatctgcg atattaacag cagaagccaa aaaagaagct atggtacgtg tagcagaagg
     2521 tgaaaaagaa tctgctatat tagtggcaga aggtgaagct gaagctataa gacagaccgc
     2581 tattgctaag gcacaaggtg aggctgaaat gataaaaaga actcaaatgg caacagcaga
     2641 aggtttaaaa ttagtttttt cagcaatgaa agaggctgat atagacaata atattctagc
     2701 attaaaatct atggaagctc ttgaaaaaat ggctgaaggt aagtcaacaa aactggtttt
     2761 accttcagaa gcagttaatt tcctaggaac attcaaagga ataaaggaag ttatgagtga
     2821 tgataacaaa gaagtacttg atataaaaga agttttaaat gataatgaat cattaaaaaa
     2881 ataaaagagt tttgttatca taggaaggat atggaaatta aaggtcgaat taattaaata
     2941 tagcacaaaa atgcacgttg aaaagaagag gacttataaa tttttaagtc ttcttctttg
     3001 tttttattag aaagatgaaa ttataaaaat gaattaacag aatctttata caatttatcc
     3061 tttactaatg taaaaagtgt tatataatat tatataaaac a
//
LOCUS       DAELSY010000259         3098 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.261, whole genome shotgun sequence.
ACCESSION   DAELSY010000259 DAELSY010000000
VERSION     DAELSY010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.261"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            192..530
                     /locus_tag="KPG09_003734"
     CDS             192..530
                     /locus_tag="KPG09_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0968287.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            552..869
                     /locus_tag="KPG09_003735"
     CDS             552..869
                     /locus_tag="KPG09_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0968288.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1019..1696
                     /locus_tag="KPG09_003736"
     CDS             1019..1696
                     /locus_tag="KPG09_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968289.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(2024..2791)
                     /locus_tag="KPG09_003737"
     CDS             complement(2024..2791)
                     /locus_tag="KPG09_003737"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBG0968290.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKVAVMPQNERDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
BASE COUNT         1250 a          400 c          357 g         1091 t
ORIGIN      
        1 atgaaaatat ttctctaatt aaaaatatga tttatccata tttgtttatt gtagatacag
       61 actatacaaa aataaatagc acttatttca gtataaaaac atatatagtc tatatttaga
      121 ctaaaaaagt ataaatagtc tatataaaac aacttaaact atgaatataa tctaaagtgg
      181 agggataata attggcaaat ataggaaaaa taataggaga tagaataaaa gtctatagaa
      241 caaaattggg atatagtcaa gagtttctag cagaaaaggc aggtttacat ccaacatata
      301 ttggtcagat tgaaagagct gagacaaata catctatcaa tattataatg aaaattgcta
      361 ctgctttaga cataccactt gaattgttat ttgcaaatat tattacaaca gaaggcgttg
      421 ataactctgt acctttagaa tgttatgaaa tgattaataa attaactgaa aaagaacaaa
      481 ttgcaatgct agagttaata aagtgcatca ttaaatacaa agaaatataa gtgagggaaa
      541 tagtaacaat aatgaattat ataggtaaaa gacttaaaga agaacgaaaa aaagcaaatt
      601 taacttcaaa agaatttgca aatatggttg gagtatcgcc ttggtatata actcaaatcg
      661 aaagtggtaa aaagaatcca agtcttaaaa cattcataaa atttgtaaat atattaaata
      721 tttctgcaga tgttcttata aaagatatta cttccactgg aaaaacatat ttagaaaatg
      781 atataaatga agagttaaaa gacttaaatt caagagaact aaatctgata tcacagatta
      841 tagacatcat aaaaaagaat aaaatataat tctttatatt ttatataaaa aaaaaggtta
      901 tttgaaataa ataacctttt tttgtctttt tgtgttaaca ttgtcttata gtgctaatga
      961 gtactattag accatgttct atggccttat tttaatataa aacgattgga gataggctat
     1021 gattgtgatt gaaggtagtg ataaatttaa gattgcaaaa gaatatattg atgtagaata
     1081 tactcttttt agcaaagtaa cttttaggta tgaaaagttg aaatttaaag ataatgctga
     1141 attggaaaaa attaagatgt ttaaatataa aaatggctac atccctaata aattaaacct
     1201 ttcttttgga tatggattct cttcttataa aaagcaaata attagagaaa ctgtagatac
     1261 tttaagattg acagaaattt tttcaagcga gaacatagaa gatattaaat ttataaaaga
     1321 tggtacaaaa aaattagaaa ttagcataga gaaagttgtg aaatttaaac gtcgaaaaaa
     1381 aagaaattat gtttgttgct attgccctga tatgtataga gatataaaac tcgacaaaga
     1441 atctatcaat aagatataca acagaaaaat aaaaatagaa agagaagtta atatttttga
     1501 agatgaggat gttataataa acaagaaagt attgaagttt ccaaagtctt ggacaaagaa
     1561 tatgcaaaaa tattggttaa gtgaaaataa gtatcccata cattctactg taattgatga
     1621 tgatagatat aaatgttgta atgtacaata tacaaaaaat agagtgataa tattatatta
     1681 catatataac cattaatata cataattaca aaaaaataat gacaaatata cataatacaa
     1741 atatatcttc tactttagat aaaacagtta ttatgaaaac ttactatcta taaataatag
     1801 aaatgctagt ataccatcta taatctaatg tctagtaggt tttaatggca tattttaaat
     1861 atgccatagt tgatttataa gattttctat atcactttca aaatacacac taaatagttg
     1921 cttttcagat ataattgccc aatttaaatt ttactatctg aaaagttttt tatatttaat
     1981 ttatatcttc aaaaataccg tctaatattt ctatataatt aatttaatta atactcttat
     2041 gtaatagatt aagtactgaa tttaactctt taaatgatat ttgccctggt gctgaaacac
     2101 tttttgcagc tccaaaagtc aatgcagaac caaatatttc accacacaat ctacttataa
     2161 cacccattcc tgacattgac attgttatta ttggtctatc tgcataaatt ttaaacattt
     2221 catttgtagc ttcaagtagc actaacacat ctctttcatt ttgcggcata acagctactt
     2281 tgggtaaatc tgcgcccaat tcctgcattc tacataaacg agatacaatc tcttcttttt
     2341 ttggagtttt attaaaatca tgattagata taattacttt cacttctttt ttatgagcaa
     2401 aattaactac ttcatctatg acctcatctc ccataaataa ctctacatct attaaatcta
     2461 ctaaacctgt attagaaatt tctttattca aagtagtata ataatctctt gatattaatt
     2521 tttctccccc ttctactaca cttctaaatg tgaataataa agggatatca tgtatataac
     2581 ttcttaattc atagagaact tccttaactt ctttaatatt ttctacattc tcaaaaaaat
     2641 caactcgcca ctctataata tccaaacatg catcttttaa ctctttagcc tcttttataa
     2701 tatctttttt atttttccca ataattggta cacaaatttt aggtctacct tctccaatag
     2761 taatattttt tacttgaact tttcttttca tattctcctc cataatcaga aattctatag
     2821 gactttaaat tctattttct gcataattta attatacatt aaaaatacta tacattataa
     2881 aaatactaat ttaaaaataa tttttattct aattcttaaa acaatcccac atattttaga
     2941 aaaggtactc tataatatac aaacacttct tttttaccta aatttatttt aacattctat
     3001 aattttcaca atattcttta aattttactc atataaacaa tattattctg gatgctaata
     3061 gaattaatct ataaatcata gtataattat gcagaaat
//
LOCUS       DAELSY010000260         3076 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.262, whole genome shotgun sequence.
ACCESSION   DAELSY010000260 DAELSY010000000
VERSION     DAELSY010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.262"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1663
                     /locus_tag="KPG09_003738"
     CDS             <1..1663
                     /locus_tag="KPG09_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG0968291.1"
                     /translation="HIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSVIIC
                     FNKSIKYEKIGLFELFKISFKNSIKLLYPKNILLFIFVILIIPLVNTVLISGFIGKIQ
                     IPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESVNLI
                     KRKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCIAFE
                     EYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILLISY
                     IESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASNAKADYAEIDVQ
                     ETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVGEKI
                     PTLKEMIKYSKGKVKLLIEIKLNGHEKGDIVKKVVQLIKENNFENQCIVASMDKTILQ
                     KVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFVWTV
                     NSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            1985..2569
                     /locus_tag="KPG09_003739"
     CDS             1985..2569
                     /locus_tag="KPG09_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG0968292.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKDKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNVKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         1217 a          284 c          463 g         1112 t
ORIGIN      
        1 acatatagtc tacctaaata tggataattt tataaaagtt ttgaccaatc ccatgtcaat
       61 agttctgata atactatcag ttataatact agctttttat gcattttttg aatttacctc
      121 tgtaattatt tgttttaata aatcgataaa atatgaaaaa atagggttat ttgaactctt
      181 taaaatatca tttaagaact ctataaaatt gttatatcct aaaaatatac tattatttat
      241 atttgtgata ttaataattc cattagtaaa tacagtttta atttcagggt ttataggaaa
      301 aatacaaata ccagagtaca tattagatta tataaagtct gatataattt taaatataat
      361 ctatatgatt tttcttttaa taatctatat ttcggttatt agatggattt ttagcattca
      421 tgagataact ttaaatacag aaaattttaa aagagcaaga aaagaaagtg ttaatcttat
      481 aaagagaaag attataagaa caattattta ttcattaata ttatctttag tagtagctat
      541 tattggatat gcgatttatc atagtggaat tatatttata ggcatatgga ctaaatattg
      601 tactgatatg gaatttttga agaatgtatt tataaataga tgtattgcat ttgaagaata
      661 ttttagattg attttatcaa tattcatttt tatattggac attggattta tctcagcaac
      721 ttattatcaa tataaaggag taaatatttc taaaaataat attaaaaaag agaaaaaatc
      781 tatatcaaaa attgttttta atttagtatt aatattattg atttcttata tagagtcagt
      841 tgcattttct tcacatataa atggcatgtt taatacgtca ttttcatttt atacaacagc
      901 aattgctcat agagcaggag ctgaaatggc tccagagaat actttggcag caatagaaga
      961 agcttcaaat gctaaagctg attatgcaga aatagatgtt caagaaacta aagatggaga
     1021 acttattgta cttcatgatt caaactttaa gagaactaca ggagtagata aaaatgtttg
     1081 ggatgtaaat tataatgaag taaagacata tgatgcggga agcttttatt attatggaaa
     1141 aggttattat gatgaaaagt ttgttgggga aaagatacca acactcaaag aaatgataaa
     1201 atattctaaa ggtaaggtga aattattaat agaaataaaa cttaatggac atgaaaaggg
     1261 agacatagta aaaaaagttg ttcagttaat taaagaaaat aattttgaaa atcaatgtat
     1321 agttgcatct atggataaaa ctatattaca aaaagtaaaa aaactggatg agaatttact
     1381 gacatgctat tttatggcaa ttgcatatgg tgatttttca aagttaaatt atgttgatat
     1441 atatggaatt gaatcaactt ttgttaataa aacagtagta gaaaagattc ataaaatgaa
     1501 caaaaaaatc ttcgtttgga ctgttaatag tgacagttta attgagaaaa tgcttgacct
     1561 aaatgtagat agtattatta cagataatcc ataccttata gagagtgcaa tttattggaa
     1621 gaaaaatgga tttattagtc aagtgtcaga ttatttattt taaatattct ataattgtat
     1681 tatccagtaa aagagatatt attaaaatca ataatatctc tttttaacat aaataaggtt
     1741 gttaaattaa ggttattctt ataaaaattg tgcttatcag ctataaacaa ggaataaaag
     1801 tgaaaaaatt gctctgagat tattacagtt aaaagagttt taagatgaca gagagataga
     1861 ttgtcaactg tatatttaat aaatcttgtt atttattaaa ttttatataa gtaaaatata
     1921 taaattaata aatcttatgc tagaatatat atgaaaaaca atataaattt ggaggattaa
     1981 aaacatgaca gagcgagaaa aaatgttggc aggagaactt tacgattgtg gtgatattga
     2041 gttgttaact caatggcata aagcaaaaga cctagtaaga gattataacc aaacagattc
     2101 ctcggatttg gaaaaaaaag aaaaaatatt aaatgaatta cttggtggta aggataaaaa
     2161 cttatggatt actgcaccgt attatgtaga ttatggaaat aatatttatt ttggaaataa
     2221 ttgtgaagtc aatatgaatt gtacgttttt agatgataat aaaattatca ttggggataa
     2281 tgcactaatc gcaccaaacg tacaaatata tactgcattt catccaacta atgctggtga
     2341 tagatttggt aatgtcaaag aagatggttc atttgaattt tgtaaaactc aaacagctcc
     2401 agttactatt ggagataatg tttggattgg cgggggtgct attatcatgc caggtgttac
     2461 tattggagat aatgtagtta taggtgcagg aagtattgtt acaaaagata ttccaagtaa
     2521 tatgattgca tacggaaatc catgccgtat tattcgagaa aacaaataaa gttaaatttt
     2581 cttgcaaaat ctcaaaaaac acctttctta ctttatacat agggtatttt ccaatacttt
     2641 cacatccata ttcatttgtt aaatttttgt ggaatgtagt ttttatcact tctatcttac
     2701 atttccacca ttttttataa aagtcattcc tttatagatt tatttataat agtatatatt
     2761 ctatttgatg tttgaataag cacttatttt taagtaaaat actaactttt aggttagtat
     2821 tttgctatag tatgatgatt aagtaagaat aaaatactat aatacataaa gcttaataaa
     2881 tataattttt attttactta gatattttct tttgattagt aatcaaaagt ggttataatt
     2941 tttttaatta ataagagtaa tatttatttt atgaaagagg attatattaa tatatattta
     3001 aataggaaaa attgtaaaaa gcataaaaat aatataatta attaatgaga taaaagtgga
     3061 taaaaatatt gggagt
//
LOCUS       DAELSY010000261         3073 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.263, whole genome shotgun sequence.
ACCESSION   DAELSY010000261 DAELSY010000000
VERSION     DAELSY010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.263"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     repeat_region   1..227
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1..29
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(888..2981)
                     /locus_tag="KPG09_003740"
     CDS             complement(888..2981)
                     /locus_tag="KPG09_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0968293.1"
                     /translation="MNKRNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEQNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTILNKPIKINEFNEISNTKEGKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLIYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTQSHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         1059 a          504 c          292 g         1218 t
ORIGIN      
        1 atttacattc catatagtta atataaaacg ttttaatgaa ttaacagaag atgaaagttt
       61 tatatgtatt tacattccat atagttaata taaaaccatt gaaaatagtg acttttacga
      121 aaaatttgct aaaatttaca ttccatatag ttaatataaa acacttccta ttaaatccac
      181 attaggaaat tctccgagat ttacattcca tatagttaat ataaaacccc aaaataaact
      241 tagtattttc aacacttaca cgtatacaac tctcctaaat ttgcagtgaa cgagcaatag
      301 tgcaattgat aacgcttatc atgcactctc aacaccttat atttcaactg ttacgccaaa
      361 ttttatcaca aatatagctc actgcaaaat ttctataatt ttattatatc ataaatatat
      421 tacttttgaa tattgatacc aatttatacc ataataaaag caagaaaaaa cgtaatctat
      481 ctacattgga tttaaattct ataacttaaa gttttttaca taatatttat gcttgtttaa
      541 tcaatctaat atatcattct tgtatattgc ttttaatagt cttttttctt aaataaatat
      601 ctatctgaac aagcatagtt taattaaaaa cttttgtatt cccttaaaac tgtatctacc
      661 tcttattatt ttatttatga tatattattt ttaatctaaa aatagatatc tatttttatt
      721 atagttatcc ctttttaaaa tactttaatc atatactact cctaatttaa catcccttga
      781 aatgctattt tcttatattg caatttataa ttgatatatc ttcaatataa taatttaatg
      841 tatttttgat tttaaagctt ttcagtgata ttattgataa attagaatta attatctagc
      901 tttaaaaggt caaagatttc tgttgctgtc tgcggttttc caaataaata tccttgtatg
      961 taatctggct ttatttgttt aactaaatca tactcttctt gtgtttctac accttctaaa
     1021 catactttta ttcctacatc atgacaaatt gcaacaataa aatgtataaa tgtagcatca
     1081 aacttactct ttaatatatc tttaacaaat actctatcaa ttttcactat atctattgga
     1141 gaaaatttta aaacttctag agacgagtat ccagtaccaa aatcatccat agcaatataa
     1201 attcctaaat cttgtaaagc cttaaattta aactgtaaaa tactcatatt ttgtactgta
     1261 tgactttctg tcaattctaa aacaagattt tgataaggaa aattttcatc atctataatt
     1321 tttactatat cttcaataaa tgtgtcttct aatatttgaa cagctgaaac gtttacactc
     1381 actttaaaaa aaggatagta atcaatccat ttcctaaaag tacgtaatgc cattcgaagc
     1441 acccaagccc ctacaatatt tatcatatca ttttcttcta atattggaat aaattccaaa
     1501 ggagaaattg ctttgcattt gtcattagtc caccttaaaa gaacttctac acctattatt
     1561 ttatgacttt gtgtatctac ttgtggctgg aatcgaagac taaatcccct aaaatcatca
     1621 ttaattgatg cctttaaatc ccgcatcatt tctaatgaat acattttatt ttttaatatt
     1681 tcttgggaga aaaaaacaag cctgttttta ccatgctctt tcgcatattg taaagagtaa
     1741 ctagcgcatt tataaagttc ttttactgta tctccatgtt ttggatatat tacacaacca
     1801 gcagaaattg ttatatttaa tccatatttt ctccatagtt gaaggtgtat aatcatattc
     1861 tgtatttgat tatatagagt tagaatttca ttttcttcaa cattatcaac aagaataccc
     1921 atttcgtccc catcaagttt ataaagttca gcattatctg gtaaaataga ttgtatagac
     1981 tgtgctagtg tttttattat attatctcca aaatccctat catacatctc attaacattt
     2041 ttaaaatcgt caatatttaa taatactatg cataattttt caatcatagg atttgaaatt
     2101 ttatcttcaa atacactcat aaattcatga taattcagta actgtgtcaa tgaatcaatc
     2161 ttattttgtt tacctaattg cgtcattatt cctgcaaaaa tacttggttc tccaaattca
     2221 tcacgcatta gttgtcctct acatcttaac catatatatt ctccactacg atttttagcc
     2281 ctaaattcca ctgtatgata atccatctgg tttttaccta tttcagtgtt tgacttataa
     2341 aaacgattcc agtcttctgg atgtacaatt tttttccagt aaataagcgg attttcaaca
     2401 atttctcctg gtaagtcaaa taattcaact tgtgatggtg aatatcgaaa tacgcctgtt
     2461 ttcatattac aaatgtatat atattcatct gtacctctaa tcaaagcatc ataaagcaat
     2521 gtagaattat aatcaaaatt gtctcttgaa aaattaaaaa ctccttttcc ttctttagta
     2581 tttgatattt cattaaattc attaatctta attggtttat ttaagattgt tgagtttgca
     2641 atatctattg attgatgcat atgaacaact gtgccatctt gccatgtaat taaactatca
     2701 taattttcaa aaacacgatt taacttatta catttttcat accactttat taatactcct
     2761 tttttatcat tctttaatag ttctaaaact ttacaaaaac tacaagtaga attttgctca
     2821 ggatataata cttgccagca aacttttcct tctggatcta aaatattata ttcttctttc
     2881 attttcttgt tcataaaaat aatttcatta gtatgaatat ttgtaacata tatattaatc
     2941 tgtagttgat ttaatataac ttcaaaatta cgtttgttca tatacttatc tcctccatag
     3001 ttactgctct ttataacaac actgttaaaa ttattctttt tagaagattt acttataact
     3061 tatacataca cac
//
LOCUS       DAELSY010000262         2852 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.264, whole genome shotgun sequence.
ACCESSION   DAELSY010000262 DAELSY010000000
VERSION     DAELSY010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.264"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            325..2460
                     /gene="rnr"
                     /locus_tag="KPG09_003741"
     CDS             325..2460
                     /gene="rnr"
                     /locus_tag="KPG09_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBG0968294.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILAKILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1251 a          267 c          515 g          819 t
ORIGIN      
        1 gtaatactta tatgagatga atattttacc aatcctattt gatggtgtat aattatgaga
       61 taaagagtaa tttaagcaat tatgataaag aataacttaa gtaattataa tgatatttac
      121 aaataattta taaaacatca taatttgttt gaagttagta tgacaaagct tacattaaat
      181 caaaaatata aataagtaat tttaacttgt aataataata agaaaatata gaaaattatg
      241 tgataagtaa aaaatgtttt atctgaagaa atataaaaga caatataaat aataatataa
      301 tgaaaagtaa aacaggaggg aaaaatgata ccaggactta aagaaagttt gctaggatta
      361 ataaatgaaa gtgcatataa tcctctaaaa aaagaagaat tagcagaaat atttgatata
      421 cattcatcag aaatgccaat gttttataat tttttaaatg aattagaaga agatggttat
      481 atctgcttta ctaaaaaagg aaaaatagta tctcccaatc aaatgggata ctttgttgga
      541 aaatttgtat cacacaaaaa gggttttgga tttgtagaat ctgatgttga atatacacag
      601 gatttattta tctcaagtga caatataaat ggtgctatgc ataacgatag agttatggca
      661 gaaatagtag tacctgcaac tgaagaaaag agagcagaag gaagaatagt aaaagtaata
      721 aagagagaaa taactagaat agtaggtact ttccaatcaa gtaaaacttt tggatttgta
      781 acaccagaca acaagaaatt tactaaagat atatatatac ctaaaaaaca ttttagtgga
      841 gcagtagata atgataaggt agtatgtgaa attacagtat ggccacaaga ggatagaaaa
      901 ccagaaggca aaattataga gatactaggt caaaaaggtg aaagaggcgt agaaatagat
      961 tctataataa gagaacatgg tcttccagaa gaatttccaa agaaagtatt gcaagaagca
     1021 gaagctgtag ctgttgagat tcctcaagaa gagataaaaa gaagaagaga tttaagagat
     1081 ttaaacatat ttactataga tggagatgac gcaaaagact tagatgatgc catatcaata
     1141 gaagtattac ctaatggaaa cttcaaacta ggagttcaca tagctgatgt tacacattat
     1201 gtaagagaga aaaataaact tgataaggaa gccttaaaaa gagctacatc agtttattta
     1261 gtagataaag taattccaat gttgccaaaa actctttcaa atggagtatg tagcttaaat
     1321 ccatttgaag ataagttaac tctttctata tttatggaaa tagaccataa aggaaatgtg
     1381 gttaaacatg aaatatgtga atctgtaata aattcaaaag ctagaatgac ttatacagaa
     1441 gtatctgata tattagaaaa ggatgatgaa aaacttaaaa agacttttga acatgttgtt
     1501 gaagatttta aaaatgctga aattttagct aagattctta tgtcaagaag agaaaaaaga
     1561 ggagctatag acttcaattt cccagaggca aagataattc taaatggaaa aggcgaagtt
     1621 gtagatataa agccatatga aagaagaata tctaataaga taatagaaga atttatgctt
     1681 ataagtaatg agacaattgc cgaacatttt tactggatgg gaataccgtt tgtgtataga
     1741 atacatgaga caccatcttt ggaaaaaatg gaagaattga gtaagtttat atctacattt
     1801 ggatatataa taaaaggtga taaagaggaa gtacatccaa aagcattaca aggtataata
     1861 gagaaaataa agggcaaaaa agaagaagga gctatcagta ctataatgct tcgttcttta
     1921 aaacaagcta agtactcacc agaatgtagt ggacattttg gattagcagc taaattctac
     1981 tgtcacttta cttcaccaat aagaagatat cctgaccttc aaattcatag aataataaaa
     2041 gaatctttaa ataataaaat aagtggaaaa agacaagaac agcttactac tatagtggat
     2101 tatgcatctg ttcaatcatc cgagaaagaa agaaaggcag agttagctga aagagatgta
     2161 catgatttct ataaggctct ttatatgaag gataaagttg gtcaagaatt tgagggtgtt
     2221 gtatctagcg taacttcatt tggaatattt gttgagttag ataatactat agaaggattg
     2281 ataagacttg caaatatgaa tgatgattac tatatttatg atgagagttc ttatagtgtt
     2341 ttaggagaaa gaactaagaa gtcatataag ataggagacc ttgttaagat aaaggttgaa
     2401 agtgttaatg tagattttaa agaaattgac tttgagatag ttgaaaaaat agaggagtag
     2461 ataaaacagc taaaataaga gataacaagt tgtagtattt atgatatgta taaatatata
     2521 gtgcacaata ctctataaaa taaatgataa aaataggtaa ttaagaaata tctaatggaa
     2581 atagttaact taaagataaa taattaagaa atatttaatg aaagtagcta attgaaaaat
     2641 aagtaattga gaaatattta atagaaagag ctaactgaaa gataagtaat taagaaacat
     2701 ttaatagaaa aagccaactg aaaaataaat aattaagaaa tgtttaatgg aagtgactaa
     2761 ttgaaaaata agtaattaag aaatgcttaa tagaagtggc taattgaaaa ataagtaatt
     2821 aagaaatgtt taatagaagt ggctaattga aa
//
LOCUS       DAELSY010000263         2836 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.265, whole genome shotgun sequence.
ACCESSION   DAELSY010000263 DAELSY010000000
VERSION     DAELSY010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.265"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            113..1036
                     /locus_tag="KPG09_003742"
     CDS             113..1036
                     /locus_tag="KPG09_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBG0968295.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESINLTKTKIY
                     SNLRDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDGLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLNKKDEDIYKILSLNLLKIIALGNDNSLKGTKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1011..1838
                     /gene="panB"
                     /locus_tag="KPG09_003743"
     CDS             1011..1838
                     /gene="panB"
                     /locus_tag="KPG09_003743"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBG0968296.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGAKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIADMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGVFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1857..2705
                     /locus_tag="KPG09_003744"
     CDS             1857..2705
                     /locus_tag="KPG09_003744"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBG0968297.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNINTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PKNIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         1146 a          344 c          453 g          893 t
ORIGIN      
        1 actttaaatg tttttaatta ttttgtctta tgtaactcat atcctcagga atgagattag
       61 aaattaagat gttgtatcac aaagtggagg tgatactatt aaaataggct ttattggttc
      121 tggaaaagtt ggaacatctt tggcaaatta ttttttatca aaaggttatt gtgtttcagg
      181 cttttatgga aggaatcaat tatctttatt agaatcaata aatttaacaa aaacaaagat
      241 ttacagcaat ttgagagata taatttatga aaacgatata ttatttatta caacatcaga
      301 tgattctatt gaaattatag acaaaaaact ttccaaattt aatttaaaac ataaatacat
      361 ttgccacaca agtggctctt taaaatctag tatattgttc tgttcaaaaa aagcaggtgc
      421 attaatttat tctatccatc ctatatttgc attttccaat aaaaatacca acataaaaaa
      481 aatgaaagac atttgttttt ccatagaagg tgataatgaa caagatgatt tagttattca
      541 aaatttcata gatggtttgg gaaaccaatt ttttataaga gataaggata cttcatccac
      601 ttatcatcta gctaatgttt tagtttcaaa tttagtatta tctttactag atataggagt
      661 aagttacttt attaaattag gtttatctga agaggaatct ttaaaagcta tcagtccttt
      721 agtaaaaaaa aatatagaaa atatacttga taatggcttt actaaagctc ttactggtcc
      781 tgtttctcgt ggagatataa caccaataag gaaacatcta tctgtattga ataaaaaaga
      841 tgaggatatc tacaaaatac tatccttaaa cctactaaaa ataattgcct taggaaatga
      901 caattcatta aaaggtacaa aaaatattac tatagaaaat gcaatcgaaa atttaataag
      961 taaatctgaa aaatacaaag aaatttacaa acttttagga ggaaaaaacg atgaaaaata
     1021 ccatacagac ttttaagaac gctaaatatg aaggaaaaaa actatctatg cttactgctt
     1081 atgactactc aattgcaaaa ataatggatg aatgtgatat aaatggtatt ttaataggcg
     1141 attcattagg aatggtcata aaaggtgaag aaaacactct atctgttact atagacgaaa
     1201 ttatatatca tacaaaagct gtaaaaaatg gtgcaaaaaa tgccttaatc gtaagtgata
     1261 tgccttttct atcttaccat gtttctatag aagatgcagt taaaaacgct ggtagactta
     1321 taaaagaagg aggagctcat gctgtaaagt tagaaggtgg ctcaaacgtt ataaagcaaa
     1381 tagaaagcat tgtaaatgct cagatacctg taatggggca tttaggatta actcctcaat
     1441 cagtaaattc atttggtggg tttaaggttc aaggaaatac cagtgaaaca gcaagacaac
     1501 ttattgaaga tgctaaatta atagaaaaag caggtgcatt ctctattgtc cttgaaggag
     1561 ttcctacgaa aatagctgat atggtaacta attctatttc aattcctaca ataggtatag
     1621 gggcaggaat taactgcgat ggacaaattt tagtttatca agatatgttg ggagtgtttg
     1681 gagattttgt tcctaagttt gtaaagcagt atgcaaatat aggagatata atgaaagatt
     1741 caattaaaaa ttacatccta gaagttaata caggtgcatt tccacaagaa aaacacagct
     1801 tttcaataaa tgaatctgag ttagaaaaat tatactaata ttaaggatgg tacaaaatgc
     1861 ttgtaaaaga aattaaatta ctaagaaata taatcaaaga ttggagaaaa catggctatt
     1921 ctatagggct tgttacaaca atgggatttt tacatgaagg acaccaaagt cttataaaaa
     1981 aagctgtaaa agaaaatgat aaagtagtag tgagtgtatt tgtaaatcca actcaatttg
     2041 gaccaaatga agattttaat agctatccaa gagatataga taaagatttt aaatattgta
     2101 tggatagtgg agcaacagta gtctttaacc catcaccaga agaaatgtat ttaaaaggta
     2161 attgtactac cattaatgtt tctggactta cagattttct ttgtggagct aaaagacctg
     2221 ttcattttgg aggtgtctgc ctagttgttt caaagtttct aaatatagta acacctgata
     2281 aagcttattt tggagagaag gatgcccaac aattggctgt aattaaaaga atggtcaagg
     2341 atttaaacat aaatacagaa ataataggct gtcctataat tcgtgaaaat gatggattag
     2401 caaaaagttc gagaaataca tatctctctg aggaagaacg aaaatctgct ctaatattaa
     2461 ataagagctt aagtttagca aaagaagaat tagtaaaagg caatctaaat ccaaagaata
     2521 taaaagaatt aataacagct aaaataaatt ctgaacactt agcaaaaata gactatgttg
     2581 aaattgtaga tagtgagaca ttacaacctg ttaagcaaat agaacattct atccttgtgg
     2641 caattgctgt gtttatagga aaaaccagac taattgataa ttttacattt gagttaaata
     2701 tatagtatat aattttatat aatatatcta aaaaataata gaatacctaa aaagtaataa
     2761 attacctaaa aaataataaa ttgcttagaa aataatagat tacaataact aaaattttaa
     2821 aattaaattc cttaaa
//
LOCUS       DAELSY010000264         2726 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.266, whole genome shotgun sequence.
ACCESSION   DAELSY010000264 DAELSY010000000
VERSION     DAELSY010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.266"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..149
                     /locus_tag="KPG09_003745"
     CDS             <1..149
                     /locus_tag="KPG09_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBG0968298.1"
                     /translation="FNICVDFINSMLSNSSESFIDVFDVNTIHLGCSKECKSIKKCCK
                     RREK"
     gene            152..523
                     /locus_tag="KPG09_003746"
     CDS             152..523
                     /locus_tag="KPG09_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBG0968299.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(667..1122)
                     /locus_tag="KPG09_003747"
     CDS             complement(667..1122)
                     /locus_tag="KPG09_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968300.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1161..2426)
                     /locus_tag="KPG09_003748"
     CDS             complement(1161..2426)
                     /locus_tag="KPG09_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBG0968301.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         1002 a          388 c          368 g          968 t
ORIGIN      
        1 catttaatat atgtgtagat tttataaatt ctatgttaag taattctagt gaaagcttta
       61 tagatgtatt tgatgtaaat acaatacatc taggatgcag taaagaatgt aaatctataa
      121 aaaaatgttg taagagaagg gagaaataat aatgaaagta tgtataccag tagaggagaa
      181 taaaggattg gatagtaaac catatggaca ttttggttct gctccaattt ttgttgtgtg
      241 tgatttagaa agtggagaag ttaaatcttt agataatgga gatttagatc atgaacatgg
      301 aaaatgtcaa ccattaaaag cattgtcagg gacagctgta gatgcagtag ttgttggagg
      361 aataggacaa ggagctataa ttaaattaaa tggaatggga ataaaagttt acagagcaga
      421 aggtgatact ataagtgcta atttagactt acttaaaaat ggaaaattag tagagtttcc
      481 atcagaccat acttgctcac atgacggatg tggacatcat taaaaaaaac agctgatgaa
      541 atatatactt catcagctat tttttctttt aataaatacg attttattct tttaaaacca
      601 aaatttagat ttttatgttt tttgtagtat ccttaataat atttcaagtt tacaagttta
      661 ctacttttag attttttttg tatgtttttc tatatagtca atatcattta atatgtcatt
      721 gagttcttta ttaagtgttt ttaaatcatc accatctaca ttgtagttta aaaatagttc
      781 ctctacatta tcaatgatag aattgacttt tctagtaact tcaacaaatg tcttaaagtt
      841 actactattt ttagcattta gaaatttttt taaatccttt gatataaatg atactatttc
      901 atattcatca ttaaatagac ctctaccaat aggatatggt tcaattctct gtaaaaaata
      961 ttggtcttta gcattaatat tataagtata agatatttcc caaccgtctc ctaaatcaaa
     1021 cttgataaaa tataatgtac tacttacttt tgatattctt gtggcaccaa gctgtcttaa
     1081 aagaacttca atcgaatcac cttgaacagt attaatattc atatatacac atccccttta
     1141 gtattattat actatatttt ttaaaatttt ataattaaat aaacagtaga tataataata
     1201 gatataatca taagtggaaa acctattttg aaatactcct taaaagagat aggatgaccg
     1261 tgtttattac ctatacctgc tagtacaaca ttagcagatg ctcctattag tgttccattt
     1321 cctccaagac aagctccaag agatgttgcc caccatagtg gcattacatc aataccttca
     1381 gcttgcatag tcaaaataag aggaattagt gttgcaacaa atggaatatt atctaagaaa
     1441 gaagagacta tagctgataa ccataatatc aacaacattg taaatacaag atgtccttct
     1501 gtaagtccaa tcaatgcttg agccaatttg tttattatcc caacttctac tagtccacca
     1561 actactacaa ataatcccat aaagaataaa attgtggacc attcaatacc tgccataatt
     1621 tcatctacat cttgttttcc tattaaaagc attatagatg ctcctgtcag tgctacaaca
     1681 gaagaatcta tgtggattgt actgtgaaac ataaaaccaa aaagtatgaa tgctattaat
     1741 ataaggcttt ttataagtag aggtttatct ttaacagatt ttttttcatc tagttttaat
     1801 attgcatttt tagctctttc attcacaaca agttcttttc cataaataaa tctaaagcat
     1861 attatagtta tagcaagtat tactataaca gcaggaccta aatttattac aaaatccata
     1921 aaactaagat tggcagcact accaatcata atatttggag ggtctcctat aagagtagct
     1981 gttcctccta tatttgaagc taaaatctgt gttattaaaa atggaactgg attaagtcct
     2041 aatatttgtg taataactat agtcattggt ccaataagta agacggttgt aacattatct
     2101 aatacagctg ataaaatagc tgttataata gcaaaacaaa tcattatctt ccatgggtca
     2161 cctttagctt tctttgcagt ccagatagct atatattcaa ataaccctga gtttttgaca
     2221 acagctacaa atagcatcat accaactaat acaccaatag tattgaaatc tatatggctt
     2281 aatccttcat caagtgtaag tatattgaat attatcatta atagtgcacc accaacagct
     2341 gctacggatc tgtttacctt ttctgtaata atagctgcca taactattaa aaatatacat
     2401 atagctatta tttgttgaaa ttccataata taagtacctc catttgtatt attttgtgcc
     2461 taatgctagt actatatata gtattacttt tttattgttt tttcaatgga tgtatgaaag
     2521 tatattattt aacaaagttt ttgagaaatt gacagaattt aaagaaaatt tttgttattt
     2581 aaataaattt taaaattatt ccattgataa taatattaaa cgacaaacga aaaaatatac
     2641 cctttaaact agaatttatt gtatccaata acaattataa aatattaaat atatctatat
     2701 tataaaaata ataaaaaaag gtttga
//
LOCUS       DAELSY010000265         2679 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.267, whole genome shotgun sequence.
ACCESSION   DAELSY010000265 DAELSY010000000
VERSION     DAELSY010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.267"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..276
                     /locus_tag="KPG09_003749"
     CDS             <1..276
                     /locus_tag="KPG09_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0968302.1"
                     /translation="LSRLISDVSWSEFVRQLEYKANWYGRKIVKVGKFFASSQICNKC
                     GYKNEEVKDLNIREWICPSCNETHNRDINASINILKEGLRLITIQNK"
     gene            341..583
                     /locus_tag="KPG09_003750"
     CDS             341..583
                     /locus_tag="KPG09_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG0968303.1"
                     /translation="MQNSMKQEPCDFSHGRFRFKTSDNRVLIPVVIFLGSIVTALCDL
                     ISRVILSPVELPISTITSLLGAPIVVMLLVKRKDRA"
     gene            586..1782
                     /locus_tag="KPG09_003751"
     CDS             586..1782
                     /locus_tag="KPG09_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0968304.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(1902..2627)
                     /locus_tag="KPG09_003752"
     CDS             complement(1902..2627)
                     /locus_tag="KPG09_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG0968305.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGANFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTPFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
BASE COUNT         1019 a          311 c          444 g          905 t
ORIGIN      
        1 ctatctcgct taatttctga tgtatcatgg tcagaatttg ttcgtcaatt agaatacaag
       61 gctaattggt atggtagaaa aattgtaaag gtaggtaaat tctttgcaag ttcacaaata
      121 tgcaataaat gtgggtataa aaacgaggaa gtgaaggact taaatataag agaatggatt
      181 tgtcctagtt gtaatgaaac tcacaataga gatataaatg caagtataaa tatattaaaa
      241 gaaggattaa gactaataac aattcaaaat aaataactta tgtaagaacc gtaggaacta
      301 tggggatggc ctgtggagaa ttagtaagac atattttagt atgcaaaatt ctatgaagca
      361 ggaaccctgt gactttagtc atgggaggtt cagatttaaa acatcagata atagagtttt
      421 gataccagta gtaatatttt taggcagtat agttacagct ctttgtgatt taatatcaag
      481 agtaatttta tcaccagtag aattaccaat aagcacgata acatctttat taggagctcc
      541 aattgtagta atgttattgg tgaaaaggaa ggatagggct tagacatgtt gaaaactaat
      601 aatttaagtg taggatacaa taacaaggta gttataagta atataaatgt agaagtaaaa
      661 aatggagaaa tactatgtct tttgggaagt aatggagctg gaaaaactac cctattaagg
      721 tccttatcta aacttatatc accaataaaa ggtgaaatat atttgaatgg tgtaaacata
      781 aattgtatat caagaaaagc attatcaaaa aaaatggctt tagtacttac aaatagatta
      841 ttaggtgatt taatgactgt acaagatata gtcaatatag gaaggtatcc atatacagga
      901 ttttttggga gtctatctaa gaaagattta ataatggtag atgaagcatt ggagtctgta
      961 gatgccttac atttaaagaa aagatatttt gatgaactaa gtgatggaga aaaacaaaag
     1021 gtgttggttg caagagctct tgttcaagaa ccagaaataa ttatattaga tgagcctaca
     1081 actcacttgg atataaaaca tagattggag ctcataaaca tactgaaaaa attaagtaaa
     1141 gaaaagtcaa tatcagtaat actttcattg catgaaatag acatagcatt aaaaagttgt
     1201 gataaagttg ctcttataaa aaataataaa gtcatagctt atggtcagcc tgaagatgta
     1261 gtagatgagg atattataaa ttcgctttat gaattggatg ataaaaactt taatagtctt
     1321 ttaggttcag tagaaattag taataaatct aaaaatgaag tttttataat tggtggtggg
     1381 ggtaaagcaa caccaattta tagagctttt actaaaaaag gtataggtct gtattctgga
     1441 ataatacatg aaaatgatat tgattatgaa attggtagaa ctatggggat aaagatgttt
     1501 acagaaaatc catttgagcc gataagtgat gagagtttcg atttggctat tagaaattta
     1561 aatgattcca agattataat agatacagga tttagtgtag gagagacaaa taagaggaat
     1621 atagatatta taaaagaggc tttaaaactg gataaaaaag tttattcttt tagaaataga
     1681 gatgagagta agaagtatta tgacagtttg gatagtaaga tagaacatat agataaagtt
     1741 tctcaaataa tcaatagtgc agatataaat aatttattat aatttatgaa ataaaatgta
     1801 cttatgtgct atagaaaaaa gagtatagat tataattttt ttgaaattac aatctatact
     1861 cttttgttat tttgtttata tatttataaa actttagcat tttatagata accaatagct
     1921 tcctctctag ttaaaaaaaa gtgaatccct gtagtcgatt ctttccatct attttcatta
     1981 aaatcttcag ctacaatcgt ttctcctact ctatatataa aattttcatc agcatatgca
     2041 cgagcacttt taaaagattc tttaccatct atactagtaa ttgacagtac ttttgcctta
     2101 tcacatctac aagcatttga tgtagcagac actctttttg cgtcgctggg tattaataat
     2161 tgtaccattc tgaaattaaa acatttttta tagcctataa atgctcctgt ttcaggacaa
     2221 tacaatttaa aaaaaggggt actttcatct gatattactc catttaaagt agcatgttct
     2281 aaattggaat tttctaaatt agctcctcta atatctgagt tagataagtc acaaaatcta
     2341 aaatttgcac cacttaagtc tgcatttttt aatatagaat tttttaatga acaatccgaa
     2401 agataagaat tattaaaaat agagttttct aaatctgctt tttctaaatt taaattgata
     2461 aggtcagacc acgaaaagtc aatattactt aagcagtatc ctcttaagtc catattttct
     2521 aatcttaatt cttttaaatc tgccttttct ccagcattat tgtttttaag ccataaataa
     2581 tgtttctcaa gtgttttttc taattcttgt ttagaaatag ttttcattaa aatcaccttc
     2641 ataaatttat ttttatatat gtagtagcaa aatagtcat
//
LOCUS       DAELSY010000266         2665 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.268, whole genome shotgun sequence.
ACCESSION   DAELSY010000266 DAELSY010000000
VERSION     DAELSY010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.268"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(534..1085)
                     /locus_tag="KPG09_003753"
     CDS             complement(534..1085)
                     /locus_tag="KPG09_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0968306.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1276..2238
                     /locus_tag="KPG09_003754"
     CDS             1276..2238
                     /locus_tag="KPG09_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBG0968307.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
BASE COUNT         1034 a          277 c          434 g          920 t
ORIGIN      
        1 tatttagata taggagtgtt atgataaaag taagattatg tgttgtttat attaaatata
       61 aagtgcatat agtgataaaa caaaagtttc atataaatga agaaaaaata taaaataatt
      121 tatttaacta cttaaaaata aactaaaagg gataataaaa tagcttttag gttatggtaa
      181 taaaggtatt taaaaagagt gcataagcac tccaattgtg agaaatacat acacaaatca
      241 ttattacaac aatgaaaaat gtacatataa gagacgtttt gtatttgttt taagtgataa
      301 tattttaata ctaactaaat aattatcaca ataataaaaa agaaaaagga gtattttgta
      361 ctccatttgt caaaaatata aaacttttat ataagcttat tataacataa ttgataaaag
      421 tgtaaagcta tataatgaat ttataaagaa aaataaaata aaaaagataa atcctaatca
      481 aaggatttat ctttttgtat agaaaataat tattctatta ttaaatggca tggctaaact
      541 ggataataat ttattattac tgctttcaat gttttttctc cttgattatg gtatgtatga
      601 attgattcag ctgaaaaaca aatagagtca ttttctctaa gttgataaat agagtcattt
      661 acttctaatt ttaattgacc ttctataatc ataatatatt cctgtgattt ttcagaatga
      721 ccaacagatg tataactatg accttcttct aattccattt gaaataattc gaagtttcta
      781 gttggtgtat tgggataata acaataaaga cgataatctt tattttctga aacttgtaca
      841 tctatatcag tttttgatac aataaaagtt tcattttgag gttgttccag tattgcagta
      901 tatggaacat ttagtccact tgcaattttc caaattgtgt ttactgttgg gtttgagtct
      961 cccttttcaa tttgcgaaag cattacttta cttacgccag ataattcagc aagttgacct
     1021 agacttaagt tacgttcatt acgtaatcta tttaaatttt tagcaataac agtattaata
     1081 ctcatagtaa ctccttaaaa tgtaaattat tgacaaatat aatttacgaa tgtataattt
     1141 atttaacatt atttataatt tacaaatgta taatataatg aacgttaaat acactgtaca
     1201 tccgtaatat aaattatact gcataaagag ttgtttttca acggatgata gttgagagat
     1261 taagggggaa ttgtaatgcg tgtacttaat aatgttacag aggctatagg caatactcat
     1321 atgttacatt tatccaaaat agcagatgaa tttggagttc aaggtaatat ttatgcaaag
     1381 atggaatact taaatccggg atttagtaaa aaggaccgtg tagcattaca aatgattacc
     1441 gaagcagaaa atgcaggttt attagttcca ggtcaagcag ttgtggaatt aacaagtggt
     1501 aatactggaa caggactagc aatagcttgt gcatgtaaag gatataaatt tatagcgtgt
     1561 atgtcaaaag gaaattctat ggaaagagca agaatgatga aagctttagg tgctgaagta
     1621 gttcttgtag accaaatgcc taattcagtt cacggacagg tatctggtga agatttggca
     1681 cttgtagaaa ttgaggctca aaaaatagca aaagaaagaa atgcatttag agcagaccaa
     1741 tttaatttag aagcttgtaa tcatgcacat gaaaagtata cagcagaaga aatgtgggaa
     1801 caattggatg gaaatattga tgtatttgta gattttcttg gaagtggtag tacttttgca
     1861 ggatgttcaa aaacacttaa aaaacataat tcttctatca aatgttatgt tgttgaacct
     1921 gaaacagcag caatttattc aggtaaagaa ataactgacc aagggcataa aatacaaggt
     1981 ggaggatatt ctatggattt gccattagta gacaagaaac ttattgatgg ttatattcaa
     2041 gttacagacg aggaggcaac tgatgtagca agaaaattag ctaaattaga aggtgtattt
     2101 gcaggatttt cttcaggggc aaatgtgtgt gcagcaatta agttgttaaa gagcagtgaa
     2161 aaaggaaaaa atatagtttt aactcttaat gatagtggtt taaaatattt aagtactgat
     2221 ttatatgaag aattgtaata atagcacaat agttatgcaa tgttttttaa attaagtata
     2281 tagatttaaa ttataaaaag tatctcaatg tgatttttga aagttataaa agagatactt
     2341 ttttattttg taaatatgga gtatcctact tataagtatt aagctatata gatattgatg
     2401 attagctatg caataaaaaa gagtatgtgt ttttgcattt atacatcata acttggaata
     2461 atacatatta atgttctaaa tgtatttaaa acatatgata aaattagaac aaacagatac
     2521 aaggagataa acatgcaaaa gagaatttta agaaatttta tatttacggg taaaaatatt
     2581 ttaagtaaac ttgtttttat aataaaatat aattttagta cattaatttt atttgaatta
     2641 tttcataaag gattagcctt atttt
//
LOCUS       DAELSY010000267         2642 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.269, whole genome shotgun sequence.
ACCESSION   DAELSY010000267 DAELSY010000000
VERSION     DAELSY010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.269"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            206..508
                     /locus_tag="KPG09_003755"
     CDS             206..508
                     /locus_tag="KPG09_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968308.1"
                     /translation="MKYSKGFKLIYSLVEYSKKFKVIYSVMKYSKGFKLIYSLVEYSK
                     RFKLIYDVMKYSKEFKLIYSLVEYSKRFKVIYSVMKYSKRFKAIYNAVKYIQGDLK"
     gene            720..989
                     /locus_tag="KPG09_003756"
     CDS             720..989
                     /locus_tag="KPG09_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG0968309.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIGQCRKWLEETVSN"
     gene            complement(1206..1451)
                     /locus_tag="KPG09_003757"
     CDS             complement(1206..1451)
                     /locus_tag="KPG09_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBG0968310.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            1988..2548
                     /locus_tag="KPG09_003758"
     CDS             1988..2548
                     /locus_tag="KPG09_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin regulator"
                     /protein_id="HBG0968311.1"
                     /translation="MFFGNISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAE
                     PWFKMKDDEKKAIEAENQRKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYK
                     YKKVKFEGKVIQVIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGI
                     SAGTITYESTMGGKITIPGIAVEKIN"
BASE COUNT          968 a          280 c          413 g          981 t
ORIGIN      
        1 taacatagaa ttatcaaata tattgaatga atatttgact cttggaatgt gtaattaaat
       61 taagtttggt tattcgatgg aaggcaaatt tgatataaaa actccattta aagtgccaga
      121 taaaaacttt atggttatat aaaagttaaa agtaatttat agtgttatag aatattcaaa
      181 gggatttaaa gtaatctatg gtgttatgaa atattcaaag ggatttaaac taatctatag
      241 tcttgtagaa tattcaaaga aatttaaagt aatctatagt gttatgaaat attcaaaggg
      301 atttaaacta atctatagtc ttgtagaata ttcaaagaga tttaaactaa tttatgatgt
      361 tatgaaatat tcaaaggaat ttaaactaat ctatagtctt gtagaatatt caaagagatt
      421 taaagtaatc tatagtgtta tgaaatattc aaagagattt aaagcaatct ataatgctgt
      481 gaaatatatt caaggggatt taaagtaatc tatagtgtta tggaatattc aaagggattt
      541 gaagtaattt attgtgtttt gaaatattca aaaatatttt tggaatagtt tttataaatt
      601 taccagtatt aataatttta tgtttaacat ttagatgact taaaatcaac tatttgcact
      661 tttgtagtca tctaaaatca ttttataata tttttaaata atggaaagga tgtttgaaca
      721 tgtttgaatc aagatgtggt gtctgttgtg acagttgtac aaaaaaagaa caagtaaatt
      781 gcactggttg cccaactatg gaaaaaccat tttggggtgg agaatgtaag gttaaaactt
      841 gctgtgaaag taaggaactt aatcattgtg gtgaatgtga tactttccct tgtgatatgc
      901 ttttaaataa gggaaaagac caaggctttg accctatggt aaatattggg caatgtagaa
      961 aatggcttga agaaacagtt tctaattaat tgttttagaa tgctttgtta agtaatttat
     1021 ttaatccttt gttaggtagt atgtttagtc atttattgat tggtatattt atacatttat
     1081 tgatgggtat atttagccat acaaatagat ttacataaaa tttacgacta aaaaagagtt
     1141 gagtattagt ttttattatt taaacggata ttcaactctt ttttatatct aacttttttg
     1201 attttttaat ctttccaata ccaccacttt aatttttctg gttctggatt ttcagttgat
     1261 gcaaaatata ctgcacatct gtatacatat aactgacatc tgtctatgtg ctgtccacgc
     1321 attaaacatt ccttttcata catttgtgct gggtttgcat ctttaagtga tttaatagtt
     1381 gtatatccta acatgattaa agatttttct gttgctttcc ctacccctgg aattttttgt
     1441 agttctccca tacttcacct tctttctgat atattatttt tgtattatac ttagtaccag
     1501 atattttttt attatagtta atatttaatt ttttattata tcactttatt tatgctcttt
     1561 catctatcta tattttacca cctctaaagt actgaatcat ttaattacat cataatatag
     1621 ttttatacaa ataaaatact ttatgtttca tttaatatat aaaattcacc ttcaagaaaa
     1681 ttatattata atctgacatt tttacctcat ttttcaaaat atattgaatc ttcttgattt
     1741 attttgtaaa attatgctta ggggaaatat attttaggaa aatatgaata tataattttt
     1801 agtcaactag ttattttaag tttttaaatt ttaaaataaa atatatctaa taaaagggag
     1861 attgtattat gttttctaaa aaaaatgagg gtaacgaatt tagtaatgaa agaaaaggaa
     1921 gctctaagaa aataattaaa ttctttaaga gcacaaagga tattgctcta ctggcattta
     1981 ttttggtgtg ttttttggca atatatcctc accagcttgt tctgaagacc atgaggaggt
     2041 catttctaat caaacatcag ttatagattc tcaaaaaaca gaaatagaaa ctttaaatag
     2101 caaattgtct gatgctgaac catggttcaa aatgaaagac gacgaaaaga aagctattga
     2161 agctgaaaat caacgtaaag ctgaagaagc taaaaaggct gaagaacaac gtaaaaaaga
     2221 agaagaagag aagaaaggat atgatactgg tattacttat gaccaattag ctagaacacc
     2281 tgatgattat aagtacaaaa aggtaaaatt tgaaggtaag gttattcaag ttattgaaga
     2341 tggtgatgag gtgcaaataa gattagctgt gtctggaaat tatgataagg tcgtactatg
     2401 tagttataaa aaatcaataa ctccttcaag agtgttagag gatgattaca taactataag
     2461 aggtataagt gctggaacta taacttatga atcaactatg ggtggaaaaa taaccatacc
     2521 agggatagct gtagagaaaa ttaattaata ttgttttgta ttatagttaa tattttatat
     2581 tatagtcaat atgtttaaag atgtttttat aattgcaaat aaacagttac aaggctctaa
     2641 at
//
LOCUS       DAELSY010000268         2639 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.270, whole genome shotgun sequence.
ACCESSION   DAELSY010000268 DAELSY010000000
VERSION     DAELSY010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.270"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            113..586
                     /locus_tag="KPG09_003759"
     CDS             113..586
                     /locus_tag="KPG09_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0968312.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFEDIAVEQIVDGIRFTPMKLLI"
     gene            complement(955..1512)
                     /locus_tag="KPG09_003760"
     CDS             complement(955..1512)
                     /locus_tag="KPG09_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0968313.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
     gene            1770..2468
                     /locus_tag="KPG09_003761"
     CDS             1770..2468
                     /locus_tag="KPG09_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0968314.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHEYQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTILENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          994 a          324 c          317 g         1004 t
ORIGIN      
        1 ctatatatta tcataatatt ctaaatttat aaaacaaatt gtattgaatt aaatttattt
       61 aagatatata attataatta taattaaaat aataataaag gtgttgttta taatgttcaa
      121 agaatactta ataagagaat taaaagaaga tgatattaaa aatgcattag acttaatctg
      181 gaatgtattt gaagaatttg aatctccaga ttattgtgat gaaggaatta cgacatttca
      241 tcaatttata gaatataatt ctattattga aaaaatcaaa gaaaaaacac tcttcttttg
      301 gggttgtttt gactctaaaa aacttattgg agtcttagct attagaaatt taaatcatat
      361 atgtatgtta tttgtagata aaaactatca taaacatggt gttgcaaaaa aattaatgac
      421 tacaggcata caatactgta tttgtaataa atttaattta actgaaataa ctgtaaattc
      481 atcaccttat gctgttgaag tttatcataa attaggattt gaagatattg ctgttgagca
      541 aattgttgac ggtatacgtt ttacacctat gaaattgtta atataataaa ttaaataaaa
      601 agcaaagtct cttaaatatt ttatagttac tttgctttta aaccaagatt taaaataata
      661 ctatcttttt tgttttttaa taatatactt taaaagcctt actaatatga actataattt
      721 attttttgat atcctttttg attaacatat aaattaattc aattattaaa cataaactat
      781 gttgtttctc tttatatctc ttctttgtaa acaatattgg gcatttgaaa ataaatattc
      841 ttaaaatata ctgttctcct gttgattttc ctttggtaca aatgataaaa ttttaattta
      901 catgctccgt aatgaagtgt ttaataaata agttaaaata atttatttac ctccttaatc
      961 atatttttta tgaataccta ataacacagt atcaatagaa tcggctaaac cttttatagc
     1021 ttcttcttta ctcaaaagtg atttcccaag ttgcaaagtc gaataaccat gaagtacact
     1081 cattattaag tttaatactt cacctgtatt ctttgtattt agattacaag aaagaataag
     1141 tttttctaat aaggatttat attcatcaaa gatttctaat gtttcattat ttccatgcca
     1201 agtagcccac tgaattgttt cataaacacc aggattagaa attataaaat taaagtatgc
     1261 tatacttata cactttatag ctgtatctcc aaaatttcca atagtcgcct ttatcatttg
     1321 actattcatc tctctcattc ctttatgagc tacttcaagt aataaatcat ctagactttc
     1381 tatatgattg tacaaagatg gtgtacgtat tcctagcttt tctgcaataa cttttaaaga
     1441 aactttgtta agcccatctt tattaactat atctgatgta gcttgtataa ctatttctct
     1501 actaacacgc atgattttcc ctctttcatt taaaattaat tactttatta gtttagctaa
     1561 tctaattagt ttcgtcaatt attgtaattt ataaaattct ttatactctt ataaaatctt
     1621 gacagtttaa ttataattta atataatact aattgaatta gttttaatac taatataatt
     1681 agtattatat taaattatat attacaacat tattttatag tttgattaat tcaacaatca
     1741 ctaatatatc aaactgaaag gagattttta tggaaaaaaa atatttcgaa attcatggag
     1801 ttccttcaat cctttgggga aatccttcaa gtaaattata tatttacata catggacaat
     1861 gcggcaataa agaggaggct gaagcttttg ctaatattgc agttccacat gagtatcaag
     1921 ttttaagtgt tgatttgcct gaacatggag aacgtaaaag agaaaaaaat tcatttaacc
     1981 catggcatgt tgttccagaa ttagtatcta ttatggaata tgcaaagtgc cactggaatc
     2041 aaatttcgtt atatgcaaat agcattggag catggtttag tatgttaagc tttgaaaaag
     2101 agaacttaag agagtgtatt tttgtatctc caattctaga tatgcaacag ctcatttcaa
     2161 atatgatgct ttgggcaaat gtatcagaag aacagcttaa acatgaacta attatcccaa
     2221 cttcatttgg gcatactctt tcatgggaat atctacaata tgtaaaacaa catcctatca
     2281 ctcaatggaa tgtaccaaca aaaatattat atggtaaaca tgatgaactt actgaaatcg
     2341 gtgttgtaga gaaatttgta gaaaagttta attgcaacct tactattctg gaaaatggca
     2401 aacattggtt taacacacca caagaattaa attcattata tgaatggtta aatactttag
     2461 ttaaataaaa aataattata ttattaatat gattaaatta tttataaatg ttatttaatt
     2521 aattttattt tagcattatt aaaataacta cataaagaat aaattagtta ttattaaaga
     2581 aattaaaagt atatagagta ttaataattt tttaataatt agttattata gaaaaaaga
//
LOCUS       DAELSY010000269         2637 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.271, whole genome shotgun sequence.
ACCESSION   DAELSY010000269 DAELSY010000000
VERSION     DAELSY010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.271"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(117..1502)
                     /locus_tag="KPG09_003762"
     CDS             complement(117..1502)
                     /locus_tag="KPG09_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0968315.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(1610..>2637)
                     /locus_tag="KPG09_003763"
     CDS             complement(1610..>2637)
                     /locus_tag="KPG09_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG0968316.1"
                     /translation="LDNYIPYDQGITLDIFMYIWILGIIAISFYYLRTNFKFYNYIEN
                     FKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTRCKIIIPEKKYNNK
                     ELRFIFKHELIHYKRKDNLFKLLISIICIIHWFNPILYLLKKYFHELCELSCDEIVID
                     KCTTSEIKEYAYIILNTIKYQKTLKYSSFVSQFLNKKNIILKKRLNSMFNQDKKKINL
                     GLKFLLTFIIIGSIFSINSNFKQNEDVEYTIAKINYNKNGETYGTHIIDSNGNSIEPE
                     LIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFKKTIPLYDKEGIKI
                     IGRFEIN"
BASE COUNT          847 a          385 c          328 g         1077 t
ORIGIN      
        1 ttgttattat gatgtaataa tacatattaa tctctataag ttaattacta tctggtgtaa
       61 ttttatttta acaacaaaaa aaaatacaca caattaaaat attgtgtgca atacaattat
      121 tttttctctt ctaatagtct ttttaatacg ctcagaccag ctttaagttc ttctgtgctt
      181 tctgtagcac atacagctat tcttattgca gaaattggtt tttcttttcc aacagcaaaa
      241 cgttcagaag catagacctg tacaccttct ttaagtgctt gtatctcaaa tttttctgcc
      301 attattccat caggtagaat caaccatcta aatatacatt cttcatttcc caaaagatta
      361 tatccagata aatattgatt aataatcttg tttcttcttc ttgcagattt acggcgtgca
      421 ttaattaatt tatcggcttc acctgatgta atcatgcgat aagcaatttc tgttaaaaaa
      481 taagattgag atagattaat gttgtataat gcgcttgata atgcctctcg atactgttta
      541 ggtgtagaga tatatgctag ccgtaaacta ggtgcaataa tttttgataa actcgtaata
      601 taaattgttt gattgggcag ataggaagcc actggattta aatgtgtttc attaagtaaa
      661 ctatgaatag catcctcaat aacaattaaa ttgtatttag atgcaatatt tgcaatcatt
      721 tttcgtccat tttgtgacat tatatgtgta gttggatttt gataatctgg tattatgtat
      781 agacctttaa tattttcatt tttacaagca tataatagtc cttcttcact aatctcatta
      841 tcttcttgtt taattggaat tagttgaatc ccaagcattt tagctgctgt ctttattcca
      901 ggataagtta atgggtctac acctattcta tctccatgtt gaaacaaacc agctaaaata
      961 gcaacaattg cattttgtcc accacttgca ggaagtaagc tatctgatgt agtttcaagc
     1021 ccagctttat aaattaattt ggcaatagct tctttttgcc atttagcacc atctctttgt
     1081 ccatattgga atatagtttt aaaatctatt tccttaatta tatgtttaaa caaagtattc
     1141 atttcttcta gtgtgaaatc tggattcatt gtgcccattt caataaatgt aattttatta
     1201 ttactggaca tcaagaatag atttgaacgc gtatcataag atacaaaagt tccacttcct
     1261 gtaactccac ttattagacc ttttttttca caaattttaa aagctctaga gatagtactc
     1321 acattaacat ctaagaaatc tgccaattca cgttggggtg gtaatttagt acctggtaat
     1381 aaaaatccat tgttgatatc ttgttctagt tgttctgcaa gcgccttata taatatttgc
     1441 ccttttgaag gtctctttgg tttccagttc ataggataat tttcaaaaga attaattggc
     1501 ataagttttc acctcacatt attaatttga ttttttcttt atctaaaaca ataaattcta
     1561 aagatacaaa taaaaactga taggaaataa ttctatcagt ttttgtcttt taattgattt
     1621 caaatcttcc tataattttt ataccttctt tatcataaag agggattgtt tttttaaata
     1681 tattaaaatt tctttttctt gtatataaaa cagcttcttc tggtgtcttt ggttgattat
     1741 cacaatcgta caaatctctt ttttttacgt agccgacttt tttacctcta cctattgttt
     1801 gtataagttc tggttcaatg gaatttccat ttgagtctat tatatgtgta ccataggttt
     1861 ctccattttt attataatta atcttagcaa tagtatattc tacatcttca ttttgtttaa
     1921 aattggagtt gatagaaaat attgacccta ttattataaa ggttaataaa aacttaagac
     1981 ctaaattaat tttcttttta tcttgattaa acatagaatt taacctcttt tttaaaataa
     2041 tatttttttt atttagaaac tgagatacga atgatgaata cttcaaagtt ttttgatact
     2101 ttattgtatt taatattata tatgcatact ctttaatttc actcgtggta catttgtcaa
     2161 tcacaatttc atcacaagat aattcacata attcatgaaa atattttttt aataagtata
     2221 aaattggatt gaaccaatgt atgatacata ttatactaat tagaagctta aataagttat
     2281 cttttctttt ataatgaatt aattcatgtt taaagataaa tcttaactct ttattattgt
     2341 actttttttc tggaattatt attttacagc gtgttaaacc tataatggca ggggaataaa
     2401 tacctttaac cttatatatc tgaagttttt tatttatact tagttctttt gcttgttcat
     2461 ctaagatact agtaatatct atttctaaaa tttctttttt aaaattttct atgtaattat
     2521 aaaatttaaa atttgttctt aaatagtaaa aagagattgc tataattcct aatatccaga
     2581 tgtacataaa tatatctaaa gtaatgccct ggtcatatgg tatataatta tcgagat
//
LOCUS       DAELSY010000270         2598 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.272, whole genome shotgun sequence.
ACCESSION   DAELSY010000270 DAELSY010000000
VERSION     DAELSY010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.272"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(868..1905)
                     /locus_tag="KPG09_003764"
     CDS             complement(868..1905)
                     /locus_tag="KPG09_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0968317.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKKTDSKKQ
                     KNIGISQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISTSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
     gene            complement(2002..>2598)
                     /locus_tag="KPG09_003765"
     CDS             complement(2002..>2598)
                     /locus_tag="KPG09_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0968318.1"
                     /translation="GLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLKKCLRHKTNNLEHLLEGISLDLKKYLDIKVQYLSGGQRQS
                     LSLIMSSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQ
                     YGDRLIMLHKGEVVLDVKDKEKENLTVEEILEKFEYAV"
BASE COUNT          861 a          391 c          329 g         1017 t
ORIGIN      
        1 cccagtgcca taatcgataa aattaaactt tgcgtcatta cagatataat acctgacata
       61 aataaaacac tcctttgaaa atatttttat aattttaatt atttgctaat tttgtgtata
      121 aaaaaaccac atagtgtaca aaactatgtg gtaaaaacac gaataagcca catagtttaa
      181 tctatgtggc tcttatatag ttaaatgaaa actttataaa caatatagcc aacatagata
      241 caatcccaaa attttaatag tcttaagggg tctgtatcta tgttaaaacg taggtacaaa
      301 ccctaaaaat agtggctata ataataataa tattttctat tgtttaataa agtattcata
      361 attatctctc ccttttaata ttttaattag tcagaaaatt atatgtattc tcgacttgtt
      421 ataaattata aattatacaa taataaaagt caactgctaa atttaattaa aatactaaat
      481 aatgtatact cagaatactt ttaaattaat gtattgtttg caaaaaacgt tatcctaagt
      541 tataagtgaa tgtggaaatt ttatcaaaat atttactaaa tatggaataa ctagacatgt
      601 atatcaatca aatatcataa aaaaaagctg tctggagaat attaaaattc ttttggacag
      661 ctcaagtagt gattagaaag ttatataact ttcataatca gtcgtcaata tagacgactt
      721 ttatggaaaa tgaataactt tatatgcaat attcaaattg ctacaataag cacaattttc
      781 aaagggttag ggtggataag agtacttatt atataaagtt ttcatattac ctacttcata
      841 ttacctattt tttgaaatta ataagtttta ttttgcagat tttgcatttt catctgactt
      901 tatatacttt atattatctg ctgataactt ttgcatgtca agtgctttta gtgtatcttc
      961 gttaattatt atttcagttt catctaatgt agtaactggt atatcgccaa cagattttcc
     1021 ttctaatact tcaatagcaa gttctccagc cttataacct agtttttcat aatctattcc
     1081 acaacaagct aaagcaccag aagatacaga accttcttca gaggctatta taggaatctt
     1141 gttttcatta gcaactttag aaactattgg catagaagaa actattaaat tgtcagtagg
     1201 gacatataaa acatctattt tgccaactaa actagaaata gcttggttaa cttcacttga
     1261 agtgctgatt cctttttcaa ctacttcata attatttttc ttagcataat catgtagaga
     1321 atcaacctgg atttttgaat taacttcact agtattgtat ataaccccta ttttctttgc
     1381 ttttggagtc aatgttttaa ccaattctaa tgttttatca attgaaagat aatcagatgt
     1441 accagaaaca tttccacctg gtttttcaag agatttaaca agtccagctt ctacagggtc
     1501 tgtaacagca gtcattatta ttggaatatc tttagtagca ttataagctg cttgtgcaga
     1561 tggagtagat atagcatata tcaaatcttt tttgtcggat acaaacttac tagcaatact
     1621 ttgtgtagta ggcatatcat tttgtgcatt ttggaaatct atttttatat tatctccatc
     1681 tttatagcct ttatcttcga gtgctttgat gaatcctttc tttgctttat ccagagacgg
     1741 atgttccact aattgactaa tacctatatt tttttgtttt ttgctatctg tttttttatt
     1801 ttcatttgaa gcattggagc catcattttg agaacatcca gtaagcattg atacaccaag
     1861 tactcctgct ataattaaac ttaatatttt tttacttttt atcattttat tttcctccct
     1921 taaaatttaa taattatatt tgttcatata ttcatatgat agattataaa tatttctgat
     1981 taaaactaag attttaattt cttatactgc atattcaaat ttttctaaga tttcttcaac
     2041 tgttaaattt tctttttctt tatcttttac atctaataca acttcaccct tatgaagcat
     2101 tatcaaccta tctccatatt gaagagcgtg tttcaagttg tgagtaacca taagtgtagt
     2161 tatatttttt tctcttacga ttttatctgt taactctatt acttcatttg atgtttttgg
     2221 gtcaagtgct gctgtatgtt cgtctagtaa taagacctta ggacttgcta agctagacat
     2281 aattagtgat agtgattgtc tttgacctcc tgaaagatat tgtactttaa tatctaaata
     2341 tttttttaag tccaaagaaa ttccttcaag taagtgttcc aaattattcg ttttatgacg
     2401 aagacatttt tttaagttca ataatttccc tttattaagt gctagagata aattttctct
     2461 aaccgtcata gaaggacaag ttccaaggct tgggtcttga aaaactctac ttactatttg
     2521 agttctttta tactcagcta aatttgttat agtagtacta tctaaagtaa tttgaccaga
     2581 agattctttt ataagccc
//
LOCUS       DAELSY010000271         2597 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.273, whole genome shotgun sequence.
ACCESSION   DAELSY010000271 DAELSY010000000
VERSION     DAELSY010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.273"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            435..914
                     /locus_tag="KPG09_003766"
     CDS             435..914
                     /locus_tag="KPG09_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0968319.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRTLLKTKLENTEVII"
     gene            911..1690
                     /locus_tag="KPG09_003767"
     CDS             911..1690
                     /locus_tag="KPG09_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968320.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKALDKENNVLGKSKALSFEYKNGNDEISLDTANNVLEKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1940..2440)
                     /locus_tag="KPG09_003768"
     CDS             complement(1940..2440)
                     /locus_tag="KPG09_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0968321.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNTINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
BASE COUNT         1021 a          355 c          315 g          906 t
ORIGIN      
        1 aactcttgat tctcatttaa gtttcattgt tttttcttaa aagcctactt ttagtcaaaa
       61 taccgttatc tttagtcatt aattaaaaaa cttattttaa atctatacat gaagtaaata
      121 atccaaaagc attattttct aactactcaa catcaaatac ttactatttt ataattactt
      181 aatataaaca cttactataa aactaaatat ataaatggct tctaaagttt aatatatcta
      241 catggcttta tatatgaggg gaaaatacta gaaaaaaata tattatagaa tatttgtata
      301 gaataggcaa aactttcaaa cttaaattta tcaaataatc ataaaaaaag tgaaccatct
      361 atagtaaaat tttatctaat aagtgaaagg caaaaaagta gcgctacgat ttgcatagaa
      421 gggaaggaac tcagttggat aaaactacat ttacaaacaa tatacttgaa tcagaacaga
      481 ctttgtatcg agtatctaag tctattctag gaaacgacca agactgtgaa gatgccgtca
      541 acaatgctat tttaaaagca tatgaaaagt tggattctct taaggaagaa caatacttta
      601 aaacatggct gattagaata gtcataaatg aatgtaattc attaagacgc aaacgattaa
      661 aatctctatc atttgaagat gtcttcaaaa acaaaaaaat tgatgaaaaa gatgactata
      721 gcgatttgta tactgctatt caaagtctac ctaaaaaaat taaaattcca atagtccttt
      781 actatattga aggttattca gtagatgagg ttaaggaaat attagatata cctcaaggaa
      841 ctgtaaaaag tagattatca agaggaagaa cattattaaa aactaaatta gaaaacacgg
      901 aggtaatcat atgaataaaa atatattaaa taattcgttt ccagaagttc cagaaagttt
      961 tcataatagt ttaagtaaaa cactaaatag tttaccagaa agagaggaaa attataaaat
     1021 gattaataat aaaacacaca aattaccatt tagaaaaggc ttagttgcaa ctttagccat
     1081 tactttgata ttgagcacaa cagcccttgc agtaggacat atatatagtt tagttggaaa
     1141 atcaagtact aaggctacct atacatctat cccaactaca gaacaagtaa aaaaagactt
     1201 taactttacc cctaatatag tttctgaatt tagtaatgga tataaattta aaggagcata
     1261 tactgtaaat aaaaaagcac ttgacaaaga aaataatgtt ttaggaaaat caaaagcact
     1321 tagttttgaa tataaaaatg gcaatgatga aatatctctt gacacagcaa ataatgtact
     1381 tgagaagagt tctgacaatc taaaagttgt agatacttac aaagatatta atatttctta
     1441 tgaagcattc gcacaaaagt ttgtaccaga aggttataaa atgacagaac aggataaaat
     1501 tgatgaaaaa tcaggcaaat acacatttag tgtagggcca aataatgaat cagacaaagt
     1561 tgaagttaat gaatttaagt ttttgacatg gaaacaagat ggagtttact attcttttac
     1621 agttcaagac tcaaatttat ctatgaatga cttagttaaa atggctcatg aagtaataga
     1681 tgcaaaataa acataagtaa taataagttg tttatgagtt tattatttat gactttaatt
     1741 tttttattag taaacatggt tgtatcttcc atctttatat tttctttaaa tttaatatat
     1801 aaaattttag atatttagct accttcaaaa gaaccattat taaaaagccc ttaaaaatat
     1861 ttcttttata tggaatttta agggctttaa ctttatttta tacaagtgta tatggaattt
     1921 taaagacttt aactttattt tatataagtg taagctccat ttgaattact tcttctttta
     1981 caggtctatc attccatgta aaaccactag atattgtatt tacctctaca aaccctaaag
     2041 atttataaac atgaatagct ctactattac ttttaaatac atcaagctca attttttcat
     2101 acccatattc ttttgataaa tcaataattt gttttaaaag actacttccg ataccttttt
     2161 ccctatactc tttaagaata cccatgccta aataaccaac tttttcagtt ttaggtaaaa
     2221 cgtcacacca accaatacag ttatctgatt ctaaatcaat tataaacagt tgtggtaagt
     2281 ttttatttat agtatttttt ataaattcaa ctgtttcttc aaaaggaaag gcttcatcca
     2341 tagctagata tttcctctct tttgctattt tatcaaaagt ttgccaatac gaataaatat
     2401 acttttcttc tgcctttata aattttattc cttctatcaa cttaacattt ctccttttta
     2461 atttatgatt gtagttaact tattttatat gattctagtt tataatgcta tataaattac
     2521 tatatattag cttaaaatat ctttgtatat ttctataaaa ttaaaaccaa tatatctatc
     2581 tcttgtatct ctattaa
//
LOCUS       DAELSY010000272         2564 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.274, whole genome shotgun sequence.
ACCESSION   DAELSY010000272 DAELSY010000000
VERSION     DAELSY010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.274"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            423..1697
                     /locus_tag="KPG09_003769"
     CDS             423..1697
                     /locus_tag="KPG09_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG0968322.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSAIYVKNATEGINF
                     LAKFFKDENPDKFIITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1904..2221)
                     /gene="trxA"
                     /locus_tag="KPG09_003770"
     CDS             complement(1904..2221)
                     /gene="trxA"
                     /locus_tag="KPG09_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG0968323.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
BASE COUNT          962 a          359 c          317 g          926 t
ORIGIN      
        1 ttgtacatct tttataacat ataaaatctt tttttctaat tatatacaat atattttcca
       61 ttatgttcta atcaatttct ttactatttt tacaacttta ttagtcatat tcataattat
      121 ttctaattag tgtttaacat tttatgtaaa atttataaaa aaatgtatat aaattaaaat
      181 aagtgactat actaaaaata tgatacggat aaacaataag ttcaacctat ctcccccaaa
      241 atcaagtgag ctacagtttc ccccaactgt agctcatttt cgtgtaattt aataaacatc
      301 tcaacaagaa ttaattaaca aaacccctta taaaaacata acatatatta aacaagctct
      361 tgattaaaac ttgagctttt ttaataaaaa acagaatagt ttattagata aggggttttt
      421 ttatgcaatt catggatgta tctaaataca ttgaaggtgc aaaccttaaa gttaaaacta
      481 aaaatggtga aactgttaaa agaatatatt ttaacaactc tgccactcca ttagttttaa
      541 aaaacgtagt ggataactta aactgtgaga ttccttggct tacttatatt aatgctccag
      601 gaataatctc agaaaaaaat actttaaaat atgaaaatgt acgctctact atcctcaaac
      661 ttattgatgg agacgaagaa aaggattctg ctatttatgt caaaaatgct acagaaggaa
      721 taaacttctt agcaaaattt tttaaagatg aaaatccaga taaatttatt attaccacag
      781 caatggagca tatggctaat tacctacctt ttaaagttaa ttttcctaca aaagttgttg
      841 gaattacaga aaaaggagaa cttgatttat gtcaattaga gaatatactt aaaaattatg
      901 ctggtaatgt atctctcgtt acagttacag gagctagtaa tgtcacaggt ataaccactc
      961 ctatatatga aatagcaaga atagctcata agtatggtgc aaagatattg gtggatattg
     1021 tgcaagtaat tcagcataaa cctttttcaa tgatgccaca tgaatgtgat gagcatatag
     1081 attttgttgt ttttagtgct cataaatgtt attctccact aaatggagga gctctggtag
     1141 gacctaaaaa attctttgaa aaatttaatc ctgtactata tggctcaggt tctaccaaat
     1201 ttgtaaatga taacaaaata ctatatgcaa acgtacccca acgttttgaa gcaggatacc
     1261 cagagtattt tggaactctt gcaatgggaa aagcactgaa cactttaaat aaaattggtc
     1321 taaacagaat ttcaaaatac gaaagagaat tatttttata tgctaaagaa gaaatgagta
     1381 aaatacctaa tataataata tatggagata aatcaaataa tgtaattatc ccaaatatat
     1441 cttttaatat aagtaatatg tattacaaag atgtagcaaa atatttagtt gataactttg
     1501 gaatagaaac tggagctgga gtagtaggtg cagatattta tgtacaaaaa ttattaggta
     1561 taagtccaaa agaagcatac gtcagattta tgagggaaaa tcctgtaggt cttgttagga
     1621 taagcttagg aatgtacaat acttttgatg aggtggatag ctttatacat gctattaaaa
     1681 ctttggcttt taaataagct ttttctattc ttttttatca ataaatagag agtagttttg
     1741 ttttcacaaa aaaacaaatt atatcaacag tcaatttcat tagcaattag acactcattt
     1801 gtaactatac aaactatttt acactataca aataaaaaac tcattacaat gagttttttt
     1861 gtttatatat gatatttatt ttaattactt caatttactt taattacata tatgattcaa
     1921 ttttgttttt taaattctcc ttaggcataa aacctattaa tctatctaca ggttttccat
     1981 ccttaaatat tagcatagtt ggtactgtag atacttcaaa tttctgagct aattctatac
     2041 tttggtctat atccaccttc aagaagtttg ctttttcaac catctcattt cctaaagctt
     2101 catatattgg tgatagcatc ttacaaggtc cacaccaagt cgcaaagaaa tctacaacta
     2161 ctaaaccatc tttattttct acttcattta taaattcatt attatttatt atttttacca
     2221 taatatacat ctcctttatt atcttcttga tactaatata ctacgcccta tataacagtt
     2281 caattaggca cttttttgta accgcctatt tttaatagat ttaatattat gataaataga
     2341 tttataagat taaatattta taagactaaa tatttctgat atattttatt tttttcttaa
     2401 tatagttgac attttctccc ttaaattata aaatcaaatt aataactaca taggtagtta
     2461 ctaatctttt atcaaaggag tttctatatg gataatataa ttaatgaatt acaaaatata
     2521 ggatttacta aatacgaatc tcaaatatac atatcattat taaa
//
LOCUS       DAELSY010000273         2502 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.275, whole genome shotgun sequence.
ACCESSION   DAELSY010000273 DAELSY010000000
VERSION     DAELSY010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.275"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1793
                     /locus_tag="KPG09_003771"
     CDS             <1..1793
                     /locus_tag="KPG09_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG0968324.1"
                     /translation="LIYSAIWLVIIIFFIFSLYHSIEHIKIISHTGTIRSETQKVVKQ
                     ELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWESMKKEIIK
                     VRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFSTISLLIWE
                     YYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILYNASNKEYVLLNIDIDNFKYINDTH
                     GYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREMLTNAIIS
                     ELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISSTVWYNENL
                     LKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPELGFLSPD
                     EFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIYQNSFYQR
                     FLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFGSGYSSLS
                     LLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIETKKDLEFL
                     NTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(1881..2462)
                     /locus_tag="KPG09_003772"
     CDS             complement(1881..2462)
                     /locus_tag="KPG09_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968325.1"
                     /translation="MLYLLKINQFERSYDIGIFKNEQSIYKFIEKIPFVKKDNSNFNY
                     TNYYMEFEDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHYWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT          919 a          279 c          348 g          956 t
ORIGIN      
        1 ttttaattta ttctgctatt tggctagtta ttattatttt ttttatattt tctctatatc
       61 atagtataga gcatattaaa attataagtc acactggtac tattcgcagt gaaactcaaa
      121 aggttgttaa gcaagaattg aataatgaaa gaaatgatga cttgataaaa cgactggata
      181 atattctgat aaagctaaga acaggtaatg gagaaaatgg atttcaaaga tgtgataata
      241 aagaatttca acaaaaacta aatcaaatgg attctatgtg ggaatctatg aagaaagaaa
      301 ttataaaagt tagaaatggt gcttcaggtg ataaattata taaattgagt gaagagtatt
      361 ctgtgttgtc taatcaaatt gtttttatat ctgaaaaaca ctctaatgca aaattgtatt
      421 cttttgctac tgcactattt atttatctta tattctcaac aataagtctt ttaatttggg
      481 aatattataa taaaaaaagg tttaaaagaa tattttatac tgataattta acaaaaataa
      541 aaaatcaggt tgcatttgaa aacagagcga ttgaaatttt gtacaatgca tctaacaaag
      601 agtatgtact gctaaatatt gatattgaca attttaaata tattaatgat acacatggat
      661 atgaatatgg agataaagtt ctaataatag ttgcagctgc actttctaaa acttttaata
      721 tcaaagaaac ttgtgcaaga attggttcag ataattttgt aatacttgca aaatatagag
      781 attctctttt agaggatatt cgggaaatgc taactaatgc tataatctct gaattagata
      841 tgaatgtaac tcaaactata tcttattgta taggagcgta cttagttgaa attgataatt
      901 taggttataa atccatcaat tctataatgg ataaggcaaa tattgcacac aaggtaagta
      961 aaacaagagg aatttcctcc acagtatggt ataatgaaaa tctgttaaaa cagttacaaa
     1021 tggaaaatag tatttataat tatatgtata aagcgataga aaatgaagaa tttcatatgt
     1081 atttgcaacc taaatttcaa atatcttctt taaatgttgt aagcgcagaa gctcttgtaa
     1141 gatggttttc tccagaactt ggttttttat caccagatga atttattcca ttgtttgaaa
     1201 aaagtggatt tattattgaa ttggactttt atatgttaaa aaaagcgtgt tcatttgtaa
     1261 aaaaaacgtt tatgaaaaaa aatcaatata catatccgat tgcagttaat ttttcaaggg
     1321 ttactattta ccaaaatagc ttttatcaaa ggtttttaga tactgttaaa gaatatgaaa
     1381 ttccttttaa gtatatagag atagaggtaa ctgaaagtgc ttttaatgaa atatctcaac
     1441 cagttatttc gattttagag gagcttaaaa agttaggatt tttaatttcg atggatgatt
     1501 ttggatcagg atactcttca ttaagtcttt tatgttctct atctattaat ggattgaagt
     1561 tagataaaag cttacttaaa gaaacattta accgggagaa agtatatagt attattcagt
     1621 gtattattga aatgtcacat cgtataggca tgtctgttgt atgtgaaggt attgaaacta
     1681 aaaaagactt ggaattttta aatacagtta aatgtgatgt aggacaaggg ttttatttct
     1741 ctaaaccgat agaagaaaaa gaatttttta acaaatatgt aactaaaaaa taatataatt
     1801 atttttatca gtctatacat tgagtcattt tatatttcgt taaatgactc aatgtattgt
     1861 tatgtatttt aaaatataaa ttatttaaaa cgttttttgt acttatatag aaattcttct
     1921 atattttttg attgttctct tatttcaact gcttctatat ctagtaaata aagtatagaa
     1981 tcatttgtaa gttcaacata ttcaccatct tctgaaccga gtgcatgtct ctcaattttt
     2041 aatccttttt cttcataata tttttttgtt tctatatata atgattctcg cttttgtatg
     2101 tagtctttca cttcattatt tggaaagtta tatgaatcca ctaatgtttc gccatttata
     2161 aaagttttct cggtagcaac tttttcatcc caataatgga tttcttccca agtaaaaaat
     2221 atctcagttc catctggaat gaatgaaaac ctggtaataa tatataaata attattgaaa
     2281 tttacttcat aatattcagg aatatcttca aattccatat agtaatttgt ataattaaaa
     2341 ttactattat ctttttttac aaagggtatt ttttcaataa atttatatat tgattgttca
     2401 tttttaaaaa ttcctatatc atagcttctt tcaaattgat taatttttaa taaatacaac
     2461 attctttctc cttttattca aaatataatt tttataaatt ta
//
LOCUS       DAELSY010000274         2499 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.276, whole genome shotgun sequence.
ACCESSION   DAELSY010000274 DAELSY010000000
VERSION     DAELSY010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.276"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(120..2309)
                     /locus_tag="KPG09_003773"
     CDS             complement(120..2309)
                     /locus_tag="KPG09_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBG0968326.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEYNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKKALPE"
BASE COUNT          789 a          452 c          238 g         1020 t
ORIGIN      
        1 ttcaccatag taaatatata cagaaaattc ttttttttac atcaattgat ttcctcagaa
       61 aaataggtgt cataaagaaa tgtatggaat cactttacaa cacctgtcat aataaaaaat
      121 cactctggta gtgctttttt atttactttt cctattggtg taactggtat tttatctatc
      181 ctcattatta ttgttggaat catatatgga ggtagatgct catttaaata ttctctaatg
      241 gttctttcat ttatcttacc actatcttcc tcatcaatta cataatatgc acatatatac
      301 atatccccgt catcatttgt tctcgatata caaacaacac ttttaatttc tgtaatatta
      361 tttattatat tttcaatctc atatagctct atcctaaact catcaatttt tacttgattg
      421 tccatccttc caatataatc taaatttcca tcatctaacc atcttactaa atcaccagtt
      481 ctatacatta acttattata ttcacaagtt ttgtaaggat tctctacaaa tacttgctct
      541 gttagccctt ttttatttaa atatcctctt gatatttgaa tgcctgatag acatagctct
      601 cctggtgctc caattggaca tagtttgttc tttttatcta atacatatgc ataagtatta
      661 aatataggtt ttcctattgg tatttttcca tattctctgt ccacatgaaa agaagtcgat
      721 acaacagtaa attcggttgg tccatactca ttatatagtt cataattacg ctttctatat
      781 ccttttaatc tttctcctcc agtctgtaca actctaagtg aattattatt gcaattttgt
      841 ataaataacc ttgcaaatgc tgttggcaat gctagtatag ttatgtcatt ttcctcacag
      901 tattcattta ctgtatattc atcaaattta gcttcttcag gcataatatc tattgatgca
      961 cctacaagca aataagggaa tagttgtttc attacagcat caaaactgaa acttaagtat
     1021 ataccacata tatcattttc tgtagcgctt atagatttta cgctataata acacatattt
     1081 actaaactct tatgttctat agctatggtc tttggcctac cagttgaccc tgaagtagat
     1141 atcgtatatg cacaatttga tgatgttatt ttttcagtta attcatatcc aagcttaaac
     1201 atttcttcat catttatatc gagtactatg gtattctcat ttacaacctt acttttgagt
     1261 tcatctgtag ttatcactac ttttgcatta ctatcttcaa gaaggtaatt caatctttct
     1321 tctggattac tcgtgtctat tggaaaaaat gctccccctg atttcataat ccctattgct
     1381 gtaataatta tgttaatatc tcttggtaac attacagcta ctatgtcttc actttccact
     1441 cctatatgat ttaaataacc tgcaagttta tcagttacct catccaattc tttatatgtt
     1501 aactttctat tttcatccct agcagctata ttactaggtg ttaccttaac ttgctcccta
     1561 aataaatcaa cataactttt atcatagggt acttcatatt gattattatt aaagtcttcc
     1621 aataatactt tttcttcttc cttacataca aattctaaat cttctattaa aatatcatta
     1681 tcttctatta tttgctctat tatataatca aattgattta aaattcttct aatagtttct
     1741 cttttatata tagatggttt atactttatt tttattttaa gtgccctctt agtatatact
     1801 attacagata aatcatatcc ttttttttct atgtcataat catatgataa atcaaaactt
     1861 ttagctataa atattgtgtt tataagctta gtatttagat gatttttatt ctctatatct
     1921 gataacaaat agtatccata ctctaaagac ctgctccact gcctttttag cttccttaca
     1981 acatctataa atttttcact ttgtctaact cttactctta ttggaattat attcattgtt
     2041 ttaccaaaaa ctacatcatc ttcatatata tacttttgaa gtataaggcc atatatagtt
     2101 tctatgatag agtctatgct aatattgttc actttagaat attgaattaa cctttcatgt
     2161 ttctcaatat tcatcttaaa aacctcatct actacttcat cagaacaatc tttataaaaa
     2221 aaatcatttg gaatttttgc tatattcgta taatcatcta acaaatccat ccaataattc
     2281 atagctcgtt ctaaattaac ttcatacata tcaaaattcc cccttactac taagtctata
     2341 gaaaaaattc ttttggaatt ttattttcac aatatataaa tatattaata atacatatgc
     2401 aagctctaaa aacatgagct tgcataaaat tacacttatt atgactacta aattcgtaat
     2461 atttacaatg tttaaaatat attataacac atatatgat
//
LOCUS       DAELSY010000275         2481 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.277, whole genome shotgun sequence.
ACCESSION   DAELSY010000275 DAELSY010000000
VERSION     DAELSY010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.277"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1170
                     /locus_tag="KPG09_003774"
     CDS             <1..1170
                     /locus_tag="KPG09_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBG0968327.1"
                     /translation="NNCILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEV
                     IKNAKIKGFYKNLVEEKDAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKIN
                     KNYLKVNPIYSNGKEIFNLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVE
                     NMYNKELNRELNKTKKSDLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIIDIDN
                     IDDSFYLSYISRCVYFNSKKLDALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLE
                     KYLNSEIFTIIIIAISNLMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLI
                     LTNIIPIGIISTRVDLSNNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            1175..1792
                     /locus_tag="KPG09_003775"
     CDS             1175..1792
                     /locus_tag="KPG09_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0968328.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKNLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            1981..2325
                     /locus_tag="KPG09_003776"
     CDS             1981..2325
                     /locus_tag="KPG09_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBG0968329.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
BASE COUNT         1073 a          238 c          370 g          800 t
ORIGIN      
        1 aataactgta ttttaaatta taatatgcta caaactcagt taggaaatct atctgagtgg
       61 gataaagcaa aaaatgttta taatcttact cttaaagata caggcgaaca atctttagga
      121 aaggaagaag taattaaaaa tgccaagatt aaaggtttct ataaaaattt ggtagaagaa
      181 aaagatgctt tcttaataga ttcaactaat tttgaaaaac ttgaagatgg aagttacatg
      241 tatgaaataa attcaaaggg taaaaaccca gaagtaagtc aatatggtaa aaatataaaa
      301 ataaataaaa actatcttaa agttaaccct atttactcaa atggcaaaga aatatttaat
      361 ctaatcgatt atgacgataa gactataaat ttattagttc ctaaaaagtt acaagtacat
      421 gaaaatgaga ttaaaaaaga gtttagagaa ttattttatt ttgaaaaaat agaagtagaa
      481 aatatgtaca ataaagagtt aaatagagag ttaaataaaa caaagaagtc agatttaaat
      541 gttaatataa tatatgtaga taataatcaa agctatttta cttataactc ccttgttatg
      601 gatgacaata ggaatctcat tgatgaccca atagcaataa ttgacataga taatattgat
      661 gattcttttt atctttctta tatatctaga tgtgtatatt ttaattctaa gaaattagat
      721 gcccttgcag atataagtaa cattattgaa gctcaaggtg tagaagcgca tatccaaagt
      781 ttacatgctg tttataatga gtatggttta gaaataaata aactagaaaa atatttgaat
      841 agtgaaattt ttactataat aataatagca atatcaaact taatgattac ctacaatata
      901 gtagctagct actatgaaag aaataaatat aagttatata ttaaaaaaat atttggttat
      961 tcaactacag ctagaagtat gttgttaata gtctcattaa tattaacaaa cattattcca
     1021 ataggcatta tatcaacaag agtagattta agtaataata ttatcttatt tggacttctg
     1081 atattagtta tagaagtaat tgtatctatt gtattagaca agattatctc aaatagttca
     1141 tttaataaaa ttataaaagg agaacattaa aaagatgatt tcattaataa atatatctaa
     1201 aaaatttaaa gataaatcta taatagacaa atttaaccta gaaatttcta agggcgagtt
     1261 tgtagcgata acaggaaata gtggttcagg taagacaact ttacttaata taatgggatt
     1321 attagaaaaa cctaattctg gtgatattat aataaataat ataaaaaatc caaatagtaa
     1381 acagataaga attttacaaa gggattttta tggctatcta tttcaaaatt atgctttaat
     1441 tgagaatgaa aatgttgaaa ataacttgaa aattgcttta aagtatcaaa aaaatatcaa
     1501 ccataatgaa aaaataaatt acgcacttga gtctgtaggt cttagaaaat acaataaaaa
     1561 aaagatatac gaattgagtg gaggagaaca acaaagggta gcattagcaa gagtaatatt
     1621 aaaagaatgt gaagtaatat ttgcagatga acctacaggt aatttagata aaaaaaatag
     1681 agacatagta tttgatatac ttaaaaatct aaataagaat ggtaaaacta tcgtatttgt
     1741 aactcatgat ttagagttat cggagcaggc agatagagtt ataaaattgt aataatatca
     1801 actataataa atataagaaa gagattacag ctaaatcaag tttaataata atacgaatag
     1861 aatttaaaca ttaagtaaat tagtagtttg taaatataag tataatatga tatcataaag
     1921 gctcaaaaga aattttatat aataagaaat ttaaaaataa caaatggagg acatataaag
     1981 atggaaaatt taccaaattg cccgaaatgt aattcagaat atacttatga agatggaact
     2041 cttttagttt gtccagaatg ctctcatgag tgggcattag gattgggagc tgaagaagat
     2101 tcaaatatta tcaaagatgt aaatggaaat gtattaaatg atggagattc tgttacagta
     2161 attagagacc ttaaagtaaa aggaagttca tcatctataa agattggaac aaaagtgaaa
     2221 aatatccgtt taatccatga ctcatcagat ggacatgata tagaatgtaa gatagatggt
     2281 tttggagcta tgaagctaaa gtcttctgtt gttaaaaaag cgtaatatta taaataagct
     2341 gtagtgttta ttgtttttca gtacagtata aaaccataag aatctattaa gtaataaaag
     2401 tttgtttatt tatctttata ttttcttatg gttttattaa atataaattt tattgaacac
     2461 caaaatattc tctcctctac a
//
LOCUS       DAELSY010000276         2430 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.278, whole genome shotgun sequence.
ACCESSION   DAELSY010000276 DAELSY010000000
VERSION     DAELSY010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.278"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            175..804
                     /locus_tag="KPG09_003777"
     CDS             175..804
                     /locus_tag="KPG09_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968330.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            805..1587
                     /locus_tag="KPG09_003778"
     CDS             805..1587
                     /locus_tag="KPG09_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0968331.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAINTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1580..>2430
                     /locus_tag="KPG09_003779"
     CDS             1580..>2430
                     /locus_tag="KPG09_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0968332.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIIGKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIKIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDGYNRDLTSEYYI
                     KLKKDIL"
BASE COUNT          968 a          223 c          432 g          807 t
ORIGIN      
        1 atgcattatt aattaaaata aattacatat actatttaat atttcattta aaatattaaa
       61 aaatgtagat aaaaattgtt ttattaagga aaaattaata tattgacaaa atttaagtgc
      121 tcatttaaac tattaattga taataatatt caatattaaa taataggagg cgttatgaat
      181 aaatatcttc taataaatcc agtggcagaa agaatgtatg gagaaaagtt tcaccttatt
      241 aaagcaaatt tgataaaaaa ggggtacata atagcagaat gtgaaccaca gttagaatat
      301 gtaaaaaagc agtacaaaga gtatacaaaa aagactgaaa atacaatgtt ggattgtaga
      361 tgtccagaaa gtataagtct tttaaaaaga aataatttga taaataattt tgaagtacct
      421 atgatagaac caatattagt aaggacttgt agggttttat acgataaata tataaaaagt
      481 aaagatgata tgttggtagt aacctgtcca tgtacacaat taagagactt tgcaagtgaa
      541 aagtttaagg ataagggtaa tgctatgttt tatacatgga aagagtttgc tgaacaagaa
      601 ggtgtaaaat cacttggaaa aatagatgaa tcaccaattc cattaggata tttcaaagat
      661 acttttgaaa aagttcttga agtatcaagt gaagacgaaa tattaagtga aatacaaaaa
      721 ataagaaatg gctttgaaga taagtatgac atagtagaaa tgttttattg taaagatggt
      781 tgtaataatg gagatggttt gtaaatggtc aataaattta ataataagtt tttaaggcta
      841 gtaaagagtg ttctgatatt ttttataatt cttttattat ggaaaattac aaattatctt
      901 ggtatttgga gtgattatat attaccatca cccgaaaagg tatatagtac atttttaaat
      961 atgataagtg atggttcaat atttattaat gtatatgcaa gtatgaaaag agttttgata
     1021 ggttttgcaa taaatactgc tataggtgtg cctttaggga tattctttgg aatttatagt
     1081 ggagtatacg aatactttaa atctctaata aattttttaa ggaatacacc tccccttgct
     1141 ttaataccaa tgcttatatt atggtttggt ataggagagg aatcaaaaat tataattata
     1201 gttttagcat cctttttccc aatctttaca agcacattga aagggattaa aaattgtgac
     1261 tcaaaactga ttgaagtggg tagagtattt gagttttcaa agcttcagat aatatttaaa
     1321 ataataatac caaatgccat tcttgatatt gctgttgggt taaagttagc tcttggatat
     1381 agctttagag caattattgg ggcagaactt gtagcagctt catctggtct tggatacttg
     1441 atatctgatg gaaaagaaat gtctagaaca gatgttgtta ttgtaggtat aatcgtaatt
     1501 ggacttttag gaataattac agattacata ttttcgatta ttgtgaagaa ggtaagtaag
     1561 ggaaagatgg tggaagcata tgaataattt agtatgtgac tctggatata taattagaaa
     1621 tttatataaa agttataaag ttgacaatga agaacatgta gttcttagtg atatctcttt
     1681 aaacatagat agaaaccata taacagtaat tttaggtgaa agtggttgtg gaaagactac
     1741 cttgcttaga gcaattgcat cacttgaagg tataaatagt ggtgagatta ggtttattaa
     1801 tgataataaa gagtatagac ctaatgttgg ttttgttttt caggagagca gattaatgcc
     1861 ttggttaaac gtaagtgaaa atatagcttt tcataatcaa agaaaaaata ctattttaaa
     1921 caaggtgctt agaaaattta aacacaataa aagaattatt ggtaaaatta taaatgataa
     1981 ttttagaagc ttagaaacaa atgataattt gaacaaatca aataaatata ataggaaaag
     2041 taatattgat gtagaaaaat atcttaagat gatgaacttg cagaaattta aaaactctta
     2101 tccaaatgaa ttgtcaggag gtatggcaca gagagtaagc atagctagag ctctttcttt
     2161 caatccagat atgttgctta tggacgagcc tttttcggca ttagattatt ttacaaggat
     2221 tgatatgcaa aatgaagtta taaaaataca taagtcaact aataaaggcg taatatttgt
     2281 aactcatgat attgatgaag cgttaaagat aggcaaaaaa atagttgttt tcacagacga
     2341 aagaaaaata aaagaattta atatagaaga tggttataat agagatttaa cttcagagta
     2401 ctatattaaa ttaaaaaaag atattttaat
//
LOCUS       DAELSY010000277         2402 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.279, whole genome shotgun sequence.
ACCESSION   DAELSY010000277 DAELSY010000000
VERSION     DAELSY010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.279"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..504)
                     /locus_tag="KPG09_003780"
     CDS             complement(<1..504)
                     /locus_tag="KPG09_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBG0968333.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIIEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYK"
     gene            complement(729..1649)
                     /locus_tag="KPG09_003781"
                     /pseudo
     CDS             complement(729..1649)
                     /locus_tag="KPG09_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
     gene            complement(1938..>2402)
                     /locus_tag="KPG09_003782"
     CDS             complement(1938..>2402)
                     /locus_tag="KPG09_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0968334.1"
                     /translation="ELSRKTIGSLNRNKARLKVARLQEHIANQRKDFLQKLSTKLIKE
                     NDIICIEDLQVKNMIKNHKLSRSISDVSWSEFIRQLEYKANWYGRKIVKVGKFFASSQ
                     ICNKCGYKNEEVKDLSIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT          776 a          338 c          291 g          997 t
ORIGIN      
        1 tttataatct ttttggtttg gaattgaacc atatggtaga ttttcatctg gaaaaacttt
       61 tacagtatta ctataagtat ttggaggatt ctctaaaaga tattttatag gaaccaaaaa
      121 agtatggtca acttcatcct tgtttatatt taaattatta atatttttta tatatcctaa
      181 gtatggatgt atgataaact ttactggtgt tataaggagg tcaagacttg atattacttc
      241 tatattgtca ctaaaagttc caagttcctc acaagtttct ctaataacag cttcatatgg
      301 agtctcacct gattctattt taccacctgg aaaagaaatt tcgctaggtt gatgcttcaa
      361 attttttgag cgaacttcaa ataatatata gtgtgtgtta tttatttcta ttattggaat
      421 taaaacagat gctctcttca tattcttgta tccatttata tatggtttgt aatttttgaa
      481 tttattttta atatcgttta tcatttttac ctcctaaaat aatattatta aaaattatat
      541 aattgagttt taacaataat atataattat gatttatcta attataagat atagtaacta
      601 tctatattat tatataatat aatgataaat atatattaac aaataataaa aaaattaatt
      661 tgattgtata taaattagtt tgttattaaa ttagtattaa aaagttcaaa aatttactta
      721 gaaagaatct ataattcgat ttctatgttt gttgagaatg caattacagc ttttccaatt
      781 atttttactt caactggctt attatctgaa ataattactt ttactaacat cttgcctttg
      841 cgttttattg cttctccttg aactgcttca aatataaact ctgtgttggg tataggtatt
      901 actttataat gtacaaggta cgcacctaaa ggtccattag catttcctgt aacagggtct
      961 tctgaaatac caattgcagg agcaaacatt cttccatgaa cttgtagagt ttctttatta
     1021 tgtaatgtaa aaatataata tccattacat tttatttctt gacttatttt cgataattca
     1081 tataaatttg gagataaact atttaatgtt gataattcct ttattccaat cataactttt
     1141 gaatgtcctg tagatgatat ggcaatagga caatctgtac gaatatcttc atttaaaata
     1201 ccaaatgcat taagtaatct tttttgatag gtttgttgta atggtggaga tacttcaatt
     1261 tttccttgtg tcataattat ttcataatca ttatcttttt ttataatatc aactggtaat
     1321 attccagcac cagttttttg aaatactgtt ttgttattga attggttttc tactgctcta
     1381 acataatggg cagcaatagt agcatgacca catataggaa cttcattagt tggtgtaaaa
     1441 aatctgatat gtacatcata attttcttga tgatttttag ccaatttatt gaaaataaaa
     1501 gcagtttatg aattatttaa ttctcttgtt attttttgca tttgtgattc agataagcca
     1561 tcagcatttg taattacacc agctggatta cctgtgaatc tttcttttgt aaatgagtca
     1621 acttgataaa gacgaaattt tttattcata ttatataatc ctttctaaac attagattag
     1681 tttatctaca tagaaaactt gagataaatt attatttatt attatctgtt taaataaaat
     1741 taataaaata acatatgtct atattaaaat ttaaaaatat acatctaaaa aagtagtatt
     1801 atgtaaggct ctatgctaca ctgaacctcc catgactaaa gtcacagggt tcctgcttca
     1861 tagaattttg catactaaaa tatgtcttac taattctcca caggctatcc ccgtagttcc
     1921 tacggttctt acatacgtta tttattttga attgttatta gtcttaatcc ttcttttaat
     1981 atatttatac ttgcatttat atctctatcg tgagtttcat tacaactagg acaaatccat
     2041 tctcttatac ttaagtcctt cacttcctcg tttttatacc cacatttatt gcatatttgt
     2101 gaacttgcaa agaatttacc tacctttaca atttttctac cataccaatt agccttgtac
     2161 tctaattgac gaataaattc tgaccatgat acatctgaaa ttgagcgaga tagtttatga
     2221 tttttaatca tattctttac ttgtaaatct tctatgcaaa taatatcatt ctcttttatt
     2281 aatttagtag atagtttttg tagaaaatcc tttctttgat tagctatatg ttcttgaagt
     2341 cttgcaactt ttaatcttgc tttgtttcta tttaaactac cgattgtttt tctcgataat
     2401 tc
//
LOCUS       DAELSY010000278         2398 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.280, whole genome shotgun sequence.
ACCESSION   DAELSY010000278 DAELSY010000000
VERSION     DAELSY010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.280"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            107..445
                     /locus_tag="KPG09_003783"
     CDS             107..445
                     /locus_tag="KPG09_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBG0968335.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(852..2288)
                     /gene="miaB"
                     /locus_tag="KPG09_003784"
     CDS             complement(852..2288)
                     /gene="miaB"
                     /locus_tag="KPG09_003784"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBG0968336.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT          773 a          376 c          266 g          983 t
ORIGIN      
        1 atgataatct atttaataaa gtatgaagta aatgatttta atgtgtcttt atggtaaaat
       61 tactaataag taataaaatt aattaaaata tatggaggtg cttataatgt ttgctgtagg
      121 atttgttgtt ataattgcac ttgccttttt cattttaagt gtactattat ttaatggaaa
      181 agccagtatg ctactagcag gttataactc tatgagtaag aaagaaaaag cacgatacga
      241 taaaaaaaaa ttatgtagaa gttctagcat tgttactttt attgtctcta taatgctatt
      301 tattatggca tttttgggat atagaataga gagtggtcaa atggacgaaa aaaaaatggt
      361 tcccttttct ataatattta taatagtcat actttccagt gtatttttta atatcctcta
      421 ctctaacaaa aaatgtaaaa aataaatagt tgtaagttga gcagttaatt ataaaagatt
      481 gactgctttt tcatgtatat tgactactct tattaaatat tttaacattt aaaaatctat
      541 gtaaaaataa tgaatatagt acaaacttct aataatgatt ttacctttgt ggttacagtc
      601 cactatatat aatttaatat gataacatgg aattactaat gacaactaag taagctttta
      661 taaaatataa taaaacaaaa atagatgtac aatgaaatta tatcttatat caaatttatt
      721 tttattaata gcaaaaactt acattagcat aacatatgac aaattcgctt aaaataaaaa
      781 tttttaatat aatttgttct atagtacatc tatttcgatt agtttacata attttattat
      841 tttgtacaat attaattatt tactaatatc ccatttaatg aaaaagttct aggttcagta
      901 attttgacca taactaattt accaattaaa tcatcatttc caccttcgaa atttacgagt
      961 ttattttgtc tagttctacc agcaaactta ttttcattat ttttacttct tccttcaact
     1021 agaacttcaa ctattctatc tttatatcca tcatttattt ctgcagaaat cctatttaca
     1081 gcttctaaca ccctattaaa tctatcatgc tttatatctt ctggtatttg attttccatt
     1141 ttagcagcag gagttccctg tcttttagaa taaataaatg taaaagctga atcatatcta
     1201 actttttcaa ccacatctaa agtgtccaat aaatcttctt ctgtttctcc tgggaaacca
     1261 atcattaagt ctgtagaaaa agcaatacct ggaacttcct ttttagcttt ttctattatt
     1321 tctaaataat attcctttgt ataatgtcta ttcatttttt tcaaaaggct actgcttcca
     1381 cattgtattg gtagatgtaa aaactcacac accttatcac aatctctaat tgcgtatatt
     1441 acttcatcag atatatcttt tggatgagat gtcataaatc gaaccctttc aattccttct
     1501 atatcattca cttttcttaa aagttctgag aatgaaactg gattttcaag tgtcttacca
     1561 taagaatcta cattttgtcc aagtaaagtg atttctttcg ttccatttgc aacaagctct
     1621 tttatctcgt taattatatc ttcaggagtt ctacttcttt ctcttcctct tgtgtatggg
     1681 actatacaat atgtgcagaa attattgcat ccatacatta tatttacaaa tgcttttaat
     1741 tcaaattttc tattacttct aagaccttct attacttctc catccacatc ccatacatcc
     1801 actaacattt tgtcagaatt tattgattct gtaagtaatt gcggaaattt atatagatta
     1861 tgtgttccaa atataaggtc tacatgttta tatttttttc ttaattcctc tacaacatgt
     1921 ggttgttgca tcatacatcc acatacagct attttcatat ctggattttt tccttttaga
     1981 tgttttaatt gtcctaagtt accatacact tttagctctg cattttctct aactgcacag
     2041 gtgttatata ttattaaatc actttcttca accatcattg acatttggta tcccatctct
     2101 tctaacatcg aacataattt ttctgaatca tgttcattca tttgacaccc aaaggtttga
     2161 gtaaacacga gttttctctt tccagttctg ttgaagtatt cttcattttg tctgtgtatt
     2221 tgttctatat atttatcttg ttcttgtatt ttctctatag gtattgttac ttgttttctt
     2281 ttgctcacgt ctattcctcc tagttaattt cttatattac acataacctt aattatatca
     2341 aataagaaaa atcttttaaa gatattatta tttttctagt tgtgttttaa tagtaaat
//
LOCUS       DAELSY010000279         2392 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.281, whole genome shotgun sequence.
ACCESSION   DAELSY010000279 DAELSY010000000
VERSION     DAELSY010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.281"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            133..1149
                     /locus_tag="KPG09_003785"
     CDS             133..1149
                     /locus_tag="KPG09_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG0968337.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            1503..2207
                     /locus_tag="KPG09_003786"
     CDS             1503..2207
                     /locus_tag="KPG09_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBG0968338.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVIICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT          985 a          226 c          435 g          746 t
ORIGIN      
        1 attaattata aatatagaga aaattagtga aaatatcctt tataaaattg acccttaagc
       61 ttacaatttt gttctttgat ttattttgta gaacaatgta taatgtacct tatatgaaga
      121 agggagacta aaatgggtta ttacaatgat aatgataata ataaaataag acgtaaaaaa
      181 attagtaaca acaaagatag taataaaaaa tctaaacatt cttatacttc ggcaaataaa
      241 aaaaataaaa gtcctaatac aagttccagt aaaagaatga gtgaaaaagt agattcacag
      301 aatcttataa aaagagatag agatagtttt acaagagata atgtaagacc tttaaataca
      361 gattacaatc aaagagaaag aagaagagtc gagtataaac aaagagagaa aaaattaaaa
      421 agtaaaaaaa gaaaaaaaca aaaaaggatt agaatagtta gggcattatt atctattatg
      481 ttagttgcac taattatatt ttcagtggca ggtgcaatat ttacagtttc agctataaaa
      541 gggtcaccag aagttactaa aaagttaatt aggtctaaat atataagtag tgaggttgta
      601 agtataaaac aaatgccaaa tgatttaaaa catgcaatag tttccataga agatgagaga
      661 ttttataagc ataaaggagt agatgttatt tctctagtta ggtcagtatt aaacaatgtg
      721 tttacagata caacacaagg tgcaagtact atagatatgc aagtttcaaa aaacctgctt
      781 actagtaatg ataaaactat gaagcggaaa ataagggata tgtataatgc taagcagatg
      841 aataagatta tgactaagga tgaaattctt gaggcatatt taaataatat gtatctaggt
      901 aaaaatgcat atggagctgc aaagggagct gaagtctact ttggtaaaaa agttagtgaa
      961 ttaaatttag ctgagtgtgc tatgttagct ggtatgacaa ataatcctgc aaggtatatt
     1021 gaccatggag aggctaagaa gagacaagtg attgtactgt ataagatgca tcagcttggt
     1081 tatataactg ataaggagta tagaatagca aaagctgatg acactccatt taaatcagaa
     1141 attgattaaa attgtaagaa tatagtattt tcaatagttt aaacaaacaa aaatgtaata
     1201 aaaaaggtga tttgccaccc atttgccacc ataattataa tctaatggca aattgtttta
     1261 atgtacaaac tagataatag aatattgttt taaatgctat attatgctaa aatttaattg
     1321 tattttgtaa attaaaaaac ttataattta gttttctagg gttccgcatt ttataatggt
     1381 caggtccaag agaaaacgcg caattgttgt gtacacggaa ggataaaagc ctgggagata
     1441 tttaataata tttctatagg cttttttatt tttacatatt ttaaatttga ggaggttttc
     1501 taatgaaaaa atttgttatt gaggatgcat tttggaatct atttcctagt gcaaaaattg
     1561 gagttattat ttgtaatgat atagataact ctataaaaga tgaagactat aagaaaataa
     1621 ttttgaaggg agaagaagaa gcattaaaat acttggagga ctcagaattt agcaacaacc
     1681 aagtgattaa agtatggaga gaggcattta aaaaatttaa aacgaaaaaa ggagcaaggt
     1741 cttctattga agcattatta aaaagagttc ataatggaaa taatatagga actattaacc
     1801 ctttagtgga tatctacaat tttatctcat taaaatatgc attaccttgt ggaggggagg
     1861 atatagataa atttatagga gatattaggc taactagagc aattggtaat gaggagttta
     1921 tcccactagg gacagatgaa aattcatttc catatgaagg agaaataatt tataaagatg
     1981 atggtggagc aatttgtaga tgttggaatt ggcgtgaagc agtgagaaca atgcttactg
     2041 aagatactaa caatgccttt ttatgcattg aattagtaga tgaaagtaga tttaaagaat
     2101 ttgaaaatgc tttaaatgaa ttagctaaaa tagttcaaga aaaattgggt gggaattgta
     2161 aaatttcaat tttagatatt gataataaag aggtatcagt ggattaatct ggagatgctg
     2221 ttgtaatgga ctattctagt tataaagata cttatattta gggtaataag ttctatttta
     2281 gatgtaacga ttatcaaagg atataatata gaaaataaaa atgtagaata taaaaagcct
     2341 ttaaaataag aaaaaaatgt attttaaagg cttttattat actctttttt at
//
LOCUS       DAELSY010000280         2353 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.282, whole genome shotgun sequence.
ACCESSION   DAELSY010000280 DAELSY010000000
VERSION     DAELSY010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.282"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            259..516
                     /locus_tag="KPG09_003787"
     CDS             259..516
                     /locus_tag="KPG09_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0968339.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENNV"
     gene            965..2203
                     /locus_tag="KPG09_003788"
     CDS             965..2203
                     /locus_tag="KPG09_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG0968340.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISVLSDKGMTNKKNYFNHNHGIQKRNKSATKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         1023 a          198 c          373 g          759 t
ORIGIN      
        1 ttttattaaa tttaatctgt aaataaaaaa gttaaaagtc caatattaac attttacttt
       61 taaaccatta aaatctttat taaagctaac aaagttataa gttatgagaa attttattaa
      121 agttaataca aaaattaatt ttaaagctaa gaaatttttt attagattca ataaaactaa
      181 taaatatagt ttagtcaata atgtattaaa taaaaacttt gttaagtcta taaaaatatc
      241 ttaaaggagg tgtaacttat gaagataaaa gtggaggata taccagttca atttcatgca
      301 atggtagaaa taatcggaat tgataagttc atagaggttg ccaagttgta tggaggaaca
      361 aatacttata tacctacgta taaggggctt tttagatatg ctagaaatag ggatatagta
      421 aaacaattta atggagtcaa ccataatgaa ttggctatta gatataatat gtgtgtaagc
      481 aatattaagc gaatactcaa tgaaaataat gtttaaacat aatgaaaata gccttgttaa
      541 atgtaagagt aaagacaaag aagatgtttt ataaagtttt tttagacaaa ttaactaaat
      601 ttactactta ttaatttaaa tacattgtat attctataaa aaaatttgtt aaatactgga
      661 gatatattga gaatacaaag aaaatggtcg aaaatatttg ttggctaaaa aatagataaa
      721 atgatacaat tgcttgtata aaggggaaaa ataaataaat aaactatata aataaagaaa
      781 tagcaattaa tatgacataa tatatttata aaattaagaa tataaataga tattttaagg
      841 gattagttgt taaattagga aatattaaaa aaatatatat atttatattt aaactatttt
      901 gttatttata taaaaatact tatctagtta ctagcaatta ttagtagaaa tgggggaaat
      961 agagatgaat aagggatttt acaaattaaa ttcagtggta ttgaactttg atattaagaa
     1021 tgatgttaac aagataagat ttaatcatta tataatgaga aaagaaaaca tacaaagact
     1081 tcaaggaact ctgccaaacg gacaatttta tatgacagtg agaagagctg ctacagattt
     1141 acaactttct atttctacta taagtaggct tgtaaaagag tttttggatt tagggataat
     1201 aagattagtg tctaagggtg taaaaggtaa tcgttctgta tatagttacg taagtataga
     1261 gtttaatgga agtttatttg atgatagcaa atatatggaa cttgactgtg tatgtaatga
     1321 tgaatttatt gataatagtg gcatagagtt tgattgtgat agcaaagata attcttttaa
     1381 tggagatata aatgtagaaa ctgtgcattg cattaatgag tatttctctg atgatgatgt
     1441 agagcataat tataacaatt tccataataa agatactata aaggataaag ccatttctaa
     1501 tattagcact atcaaaaaat atattgatga atcttttgat acaaataaat cagaaaattc
     1561 taacttacaa caggagtcaa taaatttcaa ctttaatgat acatgcaatg aaatagataa
     1621 aagaacgaat aatagtatag aaaataatca aaataaaagg cctatgagat tgagtgacat
     1681 cagtgtttta agtgataaag gtatgacaaa taaaaagaat tactttaatc ataatcatgg
     1741 tatacaaaaa aggaataaat ctgcaacaaa gaaaaaagaa tccttaaata aatttaataa
     1801 aaaaaatata cagcaaagca ttataaatat gctaaataaa aaatcaggta agaattttaa
     1861 aatcgattct ccaataagca taaaacttat caatgaaagg ctaaaggagg gctatgtctt
     1921 agaagatttc tataaagtta ttgaggtaaa agtggctaag tggaaaaata ccataatgga
     1981 aatgtatata agaccagaaa ccttgtttag ctataagttt gaatcctatg taaatgaaaa
     2041 catatctatg aaaagtcaaa gtaatgttca tccatgtaaa gaaggcaata gtaatgatag
     2101 cagtaattat atacattctg attatacaac atatgatggc agtgtgcgtg agagaaaaaa
     2161 atatgagaat gtaagatact ggaatcctat atgtgaaatt taattagttt aagaatgtat
     2221 ttatgataaa taagatatta atagagaata gtgagctata gcaaatgata tatcaatagt
     2281 tggtagtaag ttatattaag caagatatta ctatcaggta gtgaattata gtgaacgata
     2341 tatcaataat tgg
//
LOCUS       DAELSY010000281         2151 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.283, whole genome shotgun sequence.
ACCESSION   DAELSY010000281 DAELSY010000000
VERSION     DAELSY010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.283"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            61..1278
                     /locus_tag="KPG09_003789"
     CDS             61..1278
                     /locus_tag="KPG09_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBG0968341.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            1440..2138
                     /locus_tag="KPG09_003790"
     CDS             1440..2138
                     /locus_tag="KPG09_003790"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBG0968342.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
BASE COUNT          821 a          251 c          338 g          741 t
ORIGIN      
        1 aattataagt ataaaagcta atcttagctt aaataaaaat tatattttta ggaggtaaat
       61 atgttaggaa aaaaatgtat ggattatcta caaacattag gaaaaataag ttcaacaaca
      121 aatggtctta caagacttat cttgactcaa gaacataaaa aatctattga tttaattagt
      181 tcatggatgg aaggtttaaa tcttgatatt gaaattgatg acattggaaa cgttataggt
      241 acatataaat caagctttcc aaatgctccc acattagtag ttgcatctca tcaagatagt
      301 gtaaagtgtg gaggtatatt tgatggtatg ttaggtataa tagtgcctct agttggtctt
      361 gaagaagcaa aacacaataa taggtcttat cctttcaata ttaagctaat tgctttcgct
      421 gaagaggaag gaactagatt tgaaacttct ttaatgggaa gtaaagtttt tgctggtact
      481 tttaaagagg aacttcttaa atctgttgat gaaaatggaa ttacacttga agaagctgta
      541 acaaagtttg gatttaatac aaaaaactta acaaatttgc atcctagaaa agatgtagat
      601 gcatatttag aatttcatat cgagcagggt cctgttttag aaaatgaatc tcttcctgct
      661 ggaattgtat caagtattac aggatttaaa agttttaaga tatcagtaaa tggtaagtct
      721 ggacatgctg gtacacttcc aatgaatatg agacttgatg ctggatgttg tgcctgtgaa
      781 tgtgtattag ctatcgaaaa agtagctaaa acgactgctg atttggttgc tactgtagga
      841 aagatgaatt tctatccatc ttcatcaaat gtggtacctg aaagagctga atttacactc
      901 gatgtacgct cttgttcaca agaaattctt gataattctg tagaaaagat atttaatgaa
      961 atatcacata tatgtgaaaa tagaaaactt aattatacaa gtgaacttgc ttttgaaaat
     1021 gttcctgtac catgttctaa taaaataaca aaaatcatag aaaaaagttt tatagattta
     1081 aatcttaatc ctttttatat ttatagtggt gctggccatg atgctcagga aatggataat
     1141 ataactgata ttggtatggt atttataaga tgtgctgggg gcgtaagtca taatcctaat
     1201 gaaagtgttt ctgtagatga cttggatact gctgtaaaaa tatttttaaa aatactagat
     1261 aatcttgatt taaaataatt tttatataag aaagtatatt aaaatatata atctaaaaaa
     1321 tcactttata tttatattat acaagtgtac aatataatct attataaaat ataattttat
     1381 gcttttatag atttttattt tatacataaa tatatttata tataaaggag ataattttta
     1441 tgaatgaaaa attaattggt attttggctg gaatgggtcc aaggtcaaca gccccttttg
     1501 ttgatttaat tattgatgag tgtcaatctc aatatggagc aaaatatgat gatgaatttc
     1561 caaagatgat gatatattca ttacctaccc cattttatat tgaccgtcca ataaatcatg
     1621 aacttatgaa agaaactata attgatggat tacaaaagct tgaatcaact ggtgtaaatt
     1681 ttattgcaat gccatgcaat tcagcacata tatactttaa agaattaaaa gagtctatta
     1741 atataccaat attaaatatt gtagaagaaa cagttaaaaa attacctact atctcacaaa
     1801 aagtaactct tttttcgact agttcaactt ttgagtctac aatttaccag aatggtataa
     1861 tacataatgg acatgaattt atatttaaag atgaatggca aataaaatta aacaatctta
     1921 ttcaaaatat aaaaatggat aaagaaaatc cacacaatat tgatatttgg aataaactta
     1981 tcgaagatgt taaaaatgaa tctatagaaa atatagtaat tgcttgtact gatttaaatg
     2041 tagttttaga aaaatcatat ccttcaataa atattgttga ttcatcaaaa tgcttggctg
     2101 aatcagttgt caataaatat ctaaaattag ttaaataaat ttttcatgca c
//
LOCUS       DAELSY010000282         2125 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.284, whole genome shotgun sequence.
ACCESSION   DAELSY010000282 DAELSY010000000
VERSION     DAELSY010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.284"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            11..1432
                     /locus_tag="KPG09_003791"
     CDS             11..1432
                     /locus_tag="KPG09_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010293346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG0968343.1"
                     /translation="MNNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNFVQFRYFIEMF
                     RLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGGPGAVFWMWLI
                     ALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFSILITICYGFV
                     FNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSEIIVPVFAGLY
                     ILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRGLFSNEAGMGS
                     APNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSSGATGIELAQE
                     ALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLNVFRVFVVGMV
                     LFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQKKSGIKDPVFI
                     ADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1652..2113)
                     /locus_tag="KPG09_003792"
     CDS             complement(1652..2113)
                     /locus_tag="KPG09_003792"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBG0968344.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGRYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
BASE COUNT          708 a          260 c          378 g          779 t
ORIGIN      
        1 tttaacgagt attaacaatg cagtagtatt tgtaaacaat tacttatggt cgtatatctt
       61 aattataatg ttagttgtaa ttggtattta ctttactatt aaaacaaatt ttgtacaatt
      121 cagatatttt atagaaatgt ttagacttct gggtgatggt acagcgaata aaaatgcaaa
      181 aaaggaagga aaaatctctt catttcaagc attttgtata agtacatctt caagagttgg
      241 aactggaaat atagctggaa tagcaattgc agtggtagca gggggacctg gtgctgtatt
      301 ttggatgtgg cttatagcat taattggttc tgcatcaagc tttgttgaga gtacattagc
      361 tcagatttac aaagtgaaag atggtcaagc ttttagaggt ggaccagcgt attatatgga
      421 acaaggtcta aataaaaaat ggatgggtat tttattctca atacttataa ctatatgtta
      481 tggttttgta tttaatgcag ttcaagcaaa tactgtttca cttgcattta ataatgcatt
      541 tggaataagt aaaatggcta tgggaatagg tcttggaatt ttaactgcaa tagttatttt
      601 tggtggagtg catagagttg caaaagtttc tgaaataata gttcctgtat ttgcaggatt
      661 atatatatta gttgctttgg ctatagttgt aatgaatata acagagatac caagtgttat
      721 agcattgata gttgaaagtg catttgattt taaaggaatg gcgattggta catttatggg
      781 agttgtaatg actggagtga agagaggtct attttctaat gaagcaggta tgggtagtgc
      841 tcctaatgct gctgcaacag cacatgtaac tcatcctgta aaacaaggtt taattcaatc
      901 tttaggtgtt tttacagata ctataataat atgtagttgt acagcattca tggtattatt
      961 atattcaggt tatagtagta gtggagcaac tggaattgaa ttagctcaag aagctttaac
     1021 tcatcatata ggaccagttg gaaatatatt tatagcagtt tgtatattct tgtttgcatt
     1081 tagttctata gttggtaatt actattatgg tgaatcaaat atggagttta tgagtggaag
     1141 taagactaaa cttaatgtat ttagagtttt tgttgtaggc atggttttat ttggttcact
     1201 tactcaagta gatgtagttt ggaatcttgc agacttattt atggcattga tggcgataat
     1261 aaacttaata gcaatagcac ttttaggaaa atatgcattt attgctttaa aagattatac
     1321 aagtcaaaaa aaatcaggaa taaaagaccc tgtgtttatt gcagatgaaa tagaaggcct
     1381 agaaaaggta tcagagtggc atagggaatt atctgaggaa catttaggat aaaaaatagt
     1441 ataaaattat aaaaccattt tgaaagacta gagtatgtat atgctttata tgaatataat
     1501 attgaattaa tttatattac tggaattttt aattccagta atattttttt attatataat
     1561 ttagctttat taaaagtttt tattattaaa aatagcttat ttatcgtaaa gtgaatgata
     1621 ttggtttttt aattattatg aatgttggta tttaatttaa agacttaaat aaatttgcca
     1681 tttctagcgc attcatagca caatcataac ctttatttcc agcttttgta cctgctcttt
     1741 caatagcttg ttcaatattt tctgtagtta cgattccaaa tataacaggt aattcactgt
     1801 ctaaagatac ttttgctatc cctttagaga cttcactaca tacataatca taatgagaag
     1861 tagcccctct tataacacag cctaaacaga ttatagcatc atacctacca cttttagcca
     1921 ttttttgagc aacaagagga atctcaaaag ctccaggtac ccaaactatt tctatatttt
     1981 ctggagaaac gtcatgtctt aaaagacagt cttcagctcc tgataaaagt tttgatgtta
     2041 taaattcatt aaaacgagaa tttattatac ctatttttaa atcttttcca ataagttttc
     2101 cttcatatat catttttatt tcctc
//
LOCUS       DAELSY010000283         2117 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.285, whole genome shotgun sequence.
ACCESSION   DAELSY010000283 DAELSY010000000
VERSION     DAELSY010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.285"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            269..>2117
                     /locus_tag="KPG09_003793"
     CDS             269..>2117
                     /locus_tag="KPG09_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG0968345.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTSKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKDLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAGITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAITVNLNGTYTKTVTINMPN
                     GDVNNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSHGGGSSSSKVDKVVLKNTITAANKLYNEAIEGTNVGEYKVGSKAIYKTAIDKAQAI
                     LDKSGVTQKEVNDAVTALNTATDTFKAGKVVAVDKTALQDAVTAATALHA"
BASE COUNT          925 a          266 c          384 g          542 t
ORIGIN      
        1 atacaaaacc atgtttttta taacaattca ttaacataaa aatcaaactt accttaaaat
       61 ataaatttac aaatttttca atcaataaga aaatttctct attccttaca taatgactgt
      121 aatgtaacaa aataattaca aattaattat gaaatcatta tagttagaaa cctctttaaa
      181 attagatatt aagtatcctt tagaattaga acgggaacaa agttaaaaaa ttaagtaata
      241 tcaaatggac aagggggaag aaaataaaat gaaaatacca aagaagattg ctgcaatgct
      301 tacagttaca atgatagctg gaagttcaac agcaggcata gctagtgcac aaactgtggc
      361 aacaaattta acagggcaag aaagatatga aactgctgtt aaaataagtc aagatggatg
      421 gaaaaatgct gatgaggtag taatcgtaaa cgactcatct atagcagatg ctctatctgc
      481 aacaccattt gcaaaagcaa aaaatgctcc aatactttta acaagtaaag ataaattaaa
      541 tgataagacg aaagcagaaa tacaaagatt aaaagctaaa aaagtatact taataggtgg
      601 aacttctgta ttaagtacaa atatagaaaa agaaataaaa gatttaaaaa tatcttttga
      661 gagaatctct ggagctgaga gatatcaaac atccttagaa ttagcaaaga aattagatgc
      721 tataagtgat gttaagaaaa tagctgtagt taatggtgaa aaaggacttg cagatgctgt
      781 aagtgtagga gcaccagctg cacaaaacaa tatgccagta atacttgctg attctaaaaa
      841 tggaacagca gttgcagaca aatttataaa agatgctgga ataactcaat cttatgtagt
      901 aggtggagag agttcaattt ctgaagctgt taaaaataaa ctaccaaata gtacaagact
      961 aggtggaaca gatagaaatg atacaaatgc aaaagtaata aaagaatttt ataaaaagac
     1021 agatttaaaa aatgcttatg taactaaaga tggtatgaat aaacaagacc aacttataga
     1081 tgcactagca gtaggtgtat taggtgctaa aaatcaatca ccagtagttt tagttggaaa
     1141 gaacttatca gcttcacaaa aatctctagt taactctaag agttttgata agataactaa
     1201 agttggtggg aatggtaatg agacagcatt taatgaaatg aagtcattac aagaagtaaa
     1261 aacagttgaa gctaaaacaa tttctgaatt aaaatcagct atagataaag ctactgctaa
     1321 tgatgttata aactttaagc caactagtga ggttaaagaa gcatttacaa tacaaactga
     1381 taaggcgata acagtaaact taaatggtac atatacaaag actgtgacta tcaacatgcc
     1441 taatggtgat gtaaataact atgcaaaagt ggatgatgta gttatagatg atgtaaaaga
     1501 tggaactttt gttaactacg gaaaaataac taacttaaag gttaatgata aaaatggagc
     1561 caagatagaa aataattcta aaggtgagat tggtagttta acagtagcat ctggagcaag
     1621 tcaagtaaaa gtgactaatg gtggtaagat aactacagtt acaaataatt ctaaaggaac
     1681 tactatagat aataaaggta caataagtag tgttaaggga gataattctc caacaataag
     1741 tggaaatagt ccaagttcta actcttcagg aggaagttca tcaagtggag gttcttcaca
     1801 tggaggagga agttcttcat caaaagtaga taaagtagtt ttgaaaaata caataacagc
     1861 agcaaataaa ttgtataatg aagcaataga gggaacaaat gtaggtgaat ataaagtagg
     1921 gtcaaaagca atatataaaa cagcaataga caaagcacag gcaatattag acaaatctgg
     1981 tgtaacacaa aaagaagtaa atgatgcagt aacagcatta aacacagcaa cagacacatt
     2041 taaagcagga aaagtagtag cagtagataa aacagcatta caagatgcag taacagcagc
     2101 aacagcatta catgcta
//
LOCUS       DAELSY010000284         2113 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.286, whole genome shotgun sequence.
ACCESSION   DAELSY010000284 DAELSY010000000
VERSION     DAELSY010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.286"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..868)
                     /locus_tag="KPG09_003794"
     CDS             complement(<1..868)
                     /locus_tag="KPG09_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBG0968346.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKDITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLR"
     gene            complement(1700..1882)
                     /locus_tag="KPG09_003795"
     CDS             complement(1700..1882)
                     /locus_tag="KPG09_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968347.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
BASE COUNT          769 a          267 c          272 g          805 t
ORIGIN      
        1 ctcttaaaac atggtctgct aaaagtggta atattataga ttttatctta cactgttcta
       61 ttcctgatgc tcctgcttct ttaaaatctt taaactcaac agtttcattt agagtttctt
      121 ctgtaatgtt cttgatatta gaatcagtca tttcgtttgt tttttcaatt aattcattaa
      181 attttatagc aaaatcattt gaagtattta ttagttcacc ttctgagggg tctagtaatc
      241 ctctcataaa cagagcatgt tccatcataa tatggtccca gaataattct atttctttag
      301 cgttttttga ctcaatatct attttatttt caaggtcaac tacataagaa cgatataaat
      361 ttgcttcatg tattatatgt tcaagaagta gagggtagtt tgatgtaaat atagtacatg
      421 atagtacacc atctaagact ctttctttaa aattaataag cccatcaagt aatcttattg
      481 catcagagtt aagttgagct acatagttca ctaagtcttg accaacttgt gggtttacac
      541 cactttgtag atttaattct cttgtagtga tgtctttgtt tatttctatc cctgtaaact
      601 cttctgtttt ttgttctgca actgatgtga gagtagttac aagctcttct gaatataata
      661 tatcaggtct aattatacca ttactagcac taacagtgta tgataataaa tcttcaaatt
      721 gctttttata gtggtcagct tccatagcaa gattataatt tttatttgtg aatcctgctt
      781 ctaaaaaaag agcatgctct ttcataattc ttgaaaaaaa taaatgtaat tctagtgata
      841 aaataacata tttttgatta tctatcataa tttcctccct ttagaaagta tcctatttat
      901 aatactttat tcgtaatatt taaatttatg tatattataa ataaattaac taaattcaat
      961 tgctactaat atcaacagtt tttattaagt tatgggcgaa aaaattatct tactgtgttt
     1021 cttattagat atagatataa tgatttaagg tacttgttgg ctattattta ttaagagcac
     1081 aagtaatact agaaattttt atgagaatta tgtaatttat aagtattatt ttgatacaaa
     1141 acaaatattt tttgtgaaac aagaactttt atagcaaaaa atgaataaat atgaagcaat
     1201 cataatttta taccacaaca attattaatt ctaatagctg cacttaatgt tttaggtatg
     1261 ggatttaaaa agtataaaaa attaagtgat aaatatattc cattatattg cttgtgcttg
     1321 gagtagtatt ttctgtatgg atgttaggat ttaatccaac ttctatttta caggggattc
     1381 tatgctgggg tgtttcaatt ggattaaatt aagtatgcaa acaatttcaa gacaatgata
     1441 aataattgga tgtgtgatag gaatatcaac aaaaaacatt attaaaaaga attattttat
     1501 ttataaaata aaacaagtaa taagaataaa taaggagcaa ctaaaatgat ttaaatatac
     1561 tagaattaaa aagagaatgt aaagttgtct agtaatggag gtaaaatatt ttaaacgata
     1621 agctatgaaa ttgatatatt aaaatgggta agaagaatat tatataggat tcttcttacc
     1681 cattttaata aatttaattt tatgccaaac cacattcgca tagtagaatc atcactctat
     1741 ggagacataa ctctacaaca tcttctccat attttttaat aaaatcacca atttgagttt
     1801 ctatgaatga atagtattct tttaaactag caatatatgc agtcattata tgaacacatt
     1861 catcaatctt atctttaaac acaactaatc cccctaaaaa acgaaaaata aaaatattca
     1921 gtaaatagca gatattattt cactcataaa tctatatagt atgatatatt gcttagatat
     1981 attttacaag tggatatgta aaactggtta ttataatcgc aaaactggat aaactttcat
     2041 tcttaaaaga aaatttatcc agtttaaagt ttaattatga atatttgagt tagtagatat
     2101 ttaatataaa tta
//
LOCUS       DAELSY010000285         2076 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.287, whole genome shotgun sequence.
ACCESSION   DAELSY010000285 DAELSY010000000
VERSION     DAELSY010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.287"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(270..1598)
                     /locus_tag="KPG09_003796"
     CDS             complement(270..1598)
                     /locus_tag="KPG09_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0968348.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGDKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGIKR"
BASE COUNT          753 a          355 c          223 g          745 t
ORIGIN      
        1 atatttgtta tatagatatc acttagtttg aaaaaaaaac actttaaaat aaagtatctt
       61 aaagcaaata attttttaga tgtttaattt tataattcaa aaactaaaaa taaagtgaat
      121 accttaaaat aaacttttta gttcatagaa cactcttttt ttataaaata aaacatctct
      181 ttaccctttt aacaataaaa aagagactta tctctatttt gagacagtct cttttatttt
      241 aattataaag catttgtaaa ctacttggtt tatcttttta ttcctctagt catataagca
      301 tacactggta agcctatagc tgttattaca agacctccta tagatatcat agtagttttc
      361 atccctgcaa aacacaattg attaactaca acaaatatac cacttgctat agctattata
      421 ggaacaatag gatataatgg aactttatac tctcttggta tatttggatg agtctttcta
      481 agtttcataa cccctataaa agttaataca tagaatatcc aagtagcaaa tacagctaag
      541 tcagttaata aattaaattg acctgacaaa gcatatatag atgcaataac agatactaat
      601 gctattgcat ttgctggaac ttgagcagaa tttaatttag aaaatatacc atactttggt
      661 aaagtcttat ctgtagctaa agtatatgcc acccttgaac cagttagtaa aaatccattt
      721 aatgctccaa acactgaaat taatatacct acagatatta tttttccacc cattgaacca
      781 aatataactt cagcaactgc tgatgcagga gaagcataat ttgctaattc attagctggt
      841 aatacccaaa gatacgctaa atttattata aaataaacag ccataacaac tgacaatcca
      901 cctattatag cttttggaag gtcttttcca ggattcttca tttctccagc taaagtacca
      961 acattcatcc atccatcaaa agcaaataat atagcaatta aaacttgacc tagtacacta
     1021 cccaggctta atccttcacc aaccataggt gtaagtattg gattatttcc accaccttta
     1081 ataaatccaa caaccattat taaaataagt ggtattaact tacaaattgt agagacagtt
     1141 tgtatgactc ctccagtttt tgaacctaac atattaagcc ctgcaactat tactataatt
     1201 ccaattgcta ttggaagtaa aagtgatggg ctaccaatta gtgctgatgc ttgttctcca
     1261 aatataacac ctaatgctgc catcatccct ggataaaata atacaatttg catccatcca
     1321 gttaaaaaac ctaatttgtc tccataaatt tcttttatat ataccatcat tccaccagtt
     1381 tttggtatcg caactgatac ttctgcagct gtaagaccag ctgttatagt tatgattcca
     1441 gctattaacc atgccaatat accaagacct ggagctcctc cagttaatgt atagactgct
     1501 tgtggtttaa aaaatacgcc cgctcctata acagaaccaa caacagttga taatgctgct
     1561 gatacgccaa gagtcttttt cagttcatta cttcccattc ttttcctcct cattaatcct
     1621 atttaatttt aatttcatag ttaaataagc atatattatg ccaatatata gaaaaatagc
     1681 ttttttattt attaaaataa aataaactct acttagcttt cgtaatcttt tacttttagt
     1741 tatgtttaaa tataaaaaaa ttataaaaac caaaaatcca aatataataa tatttataaa
     1801 taaacgtatt ttttatattg tataatatac attgtatatt tgtaacacta aaaatagatt
     1861 ataactatta agttaaattg attttgattt ttttgcaata aaatttcatt tttttcaaaa
     1921 acattttggc ttctttctgt aattttctat ctttaaggtc ttttatataa tagtatgatt
     1981 tttgcttgtt ttatagtcta ttttatatta ataaataatt tgacaattta ttataaccca
     2041 atttaaacca taaatcaaaa ctaatatttt attttt
//
LOCUS       DAELSY010000286         2010 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.288, whole genome shotgun sequence.
ACCESSION   DAELSY010000286 DAELSY010000000
VERSION     DAELSY010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.288"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            364..657
                     /locus_tag="KPG09_003797"
     CDS             364..657
                     /locus_tag="KPG09_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0968349.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            638..1279
                     /locus_tag="KPG09_003798"
     CDS             638..1279
                     /locus_tag="KPG09_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBG0968350.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVILQI
                     LMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKQNYSVGIILPWTLDDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            1523..1855
                     /locus_tag="KPG09_003799"
     CDS             1523..1855
                     /locus_tag="KPG09_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBG0968351.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
BASE COUNT          771 a          182 c          304 g          753 t
ORIGIN      
        1 catttttata cagtatattt cttatttcat tcttttctat ttaaatagga cttaactact
       61 ttttaaattg gatttagcta cttttaattt gaaattaaaa gtatatgtaa tatatataag
      121 tatatcagtc attaggtttt tgctttaaca attgtggtgc aatacacttt tgttaaatta
      181 aaaaaattag ataaaataag attatataaa agcgacttaa gtaaattgag aatcttaata
      241 tgaaaatttt caataaatat ttggaaataa ctatttagaa atttttctaa atagttattg
      301 aaataaaatg gatgttatgt tatattctat ttagatgaaa ttctaaatag cgaggtgatg
      361 gtgttggggt ttccagaaac attcaaagct ctttcagacc ctataagaag agaaatattg
      421 gttatgttga gggagaataa aatgtcagca ggagaaatta gtgaaaaatt taatatgacg
      481 aatgctacaa tttcttatca tctatctcaa ctaaaaaagg caggactcat atttgaaaca
      541 aagtataaga actttatttt ctacgaaata aatatttcag ttttcgaaga ggttatgctt
      601 tggttttcac aatttatgga gaagggagaa aaagataatg aaaaaagcga taaataaaca
      661 atttatttta tctactttaa tatgttttat accatttatt gtatcaatat atttttataa
      721 tagattacca aatgaagttg caattcattt tgataattat ggaaatccag ataattatgc
      781 tccaaaggtt atagcagctt ttggtgtacc attgttaatg ctatgtatcc atttgtatac
      841 atggtttaga cttgagaatg agcctaaaaa agctaatttt tcaaatgtta tacaaaattt
      901 atcaaaatgg ggagttgcta tactttcagt aattctacaa atactgatgg ttttatattc
      961 aacaggagtt agtttggagt ctaattttta tacaggatta attttaggaa taatagttat
     1021 tttatttgga aactatcttc ctaaatgtaa acaaaattac tcagtaggaa taattttgcc
     1081 atggactctt gatgatgaag ataattggaa taagacacac catttagctg gatggatttg
     1141 gcttattggt ggaatattat taattattaa tgcatttatt aatattcctt tttataatat
     1201 ctttgtaatc tttgtaattg taatactacc atttatatat tcttatttga tgtataaaat
     1261 ttcaggtaaa aataactaat ataattaatc actataataa tgtttggata agaataatat
     1321 atttcagaac tgagatagat aataaagaaa ttactaagat ttctatttta gttctgaaaa
     1381 aatcttactt atatgataac ttattgagat ttcaaaaaat aatataaatt aggctaatga
     1441 acataaaatg aaaatttgta tttaatattg aaaatataag tacagtgtga tactcttata
     1501 agaaaaacat aggaggtaga agatgaatac tagagaaatg aaagaatata ggggatttta
     1561 caataaaaaa ttaagtgaaa cagatgagtt ttttagagta tttggaagtc ttgatgataa
     1621 gacttattca gaaggggcta taagtaaaaa aaataaggaa ttaatgggac tagctatttc
     1681 aattttaagc cgatgtgatg agtgtatttg ttatcatatt gagggttgta ttaattctgg
     1741 agcaagtaaa gatgaaatta tagaagctat aaaaataggt gtaattggtg gcgggtctat
     1801 tacatatcca aacgcaagat ttgcaataaa aattttagaa gaatttaata tataattttg
     1861 atacacgtag tttatttaga ctaataataa tattctattt aagtctttga aattaagctt
     1921 tattttctaa gttcacttct tattaagttt ttagaattgc atatataaat ataaattttt
     1981 aaataatata gtagttttaa attatttaca
//
LOCUS       DAELSY010000287         1996 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.289, whole genome shotgun sequence.
ACCESSION   DAELSY010000287 DAELSY010000000
VERSION     DAELSY010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.289"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            62..793
                     /locus_tag="KPG09_003800"
     CDS             62..793
                     /locus_tag="KPG09_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968352.1"
                     /translation="MLNKKNVSLIVLVIVLSLFTAIFVFNNRNTNDKSKEIAKNNEEI
                     VPKNLEALSIEQLTKIRDKAIDKNLEGLREFGEDSVKQRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQSYEINEFMLKNKKNYKEWSY
                     EYIEFLIDHLDELCKFVDFDIKDVIDIIDPTIKTDLSEEQKKSLNDKLKKVSSSDTLS
                     EDIKKEIKNWENNLNSLNMNKNNSN"
     gene            1035..1982
                     /locus_tag="KPG09_003801"
                     /pseudo
     CDS             1035..1982
                     /locus_tag="KPG09_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          850 a          190 c          327 g          629 t
ORIGIN      
        1 tattctaata aactgagtta ttttttcaca aataaaatac ttattaaaaa ggagatttga
       61 tatgttaaac aaaaaaaatg taagtttaat tgttttagta atagttttaa gtttattcac
      121 tgctatattt gttttcaata atagaaatac aaatgataaa tcaaaagaaa ttgctaaaaa
      181 taatgaagaa atagtgccta aaaacttaga agctttaagt atagaacaat taacaaaaat
      241 tagagataag gctattgata aaaatttaga aggtcttaga gaatttggag aagattcagt
      301 aaaacagcgt aaagattcaa ttttaaattt tgaatttgga ttagttgctt ttgttaacaa
      361 aaattatagt aacttaacag aaaaagagaa attaataaga caaattaata ttgttaacta
      421 tgaaaactct ataaatgaca taatggatcc ttcaggttca ttctttgaat tagcaaatca
      481 aagttatgaa attaatgagt tcatgttaaa aaacaaaaaa aattataaag aatggtcata
      541 tgaatatata gaattcttaa ttgatcattt agatgaatta tgcaaatttg ttgattttga
      601 tataaaagat gtaatagata ttattgatcc tacaataaaa acagatcttt cagaagaaca
      661 aaagaaatct ttgaatgata aattaaagaa agtgtcttca agtgatactt taagtgaaga
      721 cattaagaag gagataaaaa attgggaaaa caatttgaat agtttaaata tgaataaaaa
      781 taattctaat taactgatag tgtaaaatgt acataattat aaataaaata gggatgtctt
      841 aaaattgatt attttctaat ctaaatattt taggcctatc taaaaacata agattatggt
      901 ctaaaatttg aaattttaga ccatgcttta tctttacaat aaatgggtct ttaaaataaa
      961 gtgtgtattc ttctaaaaag tggtgataat taaaatatta aatatcacta tataaaggaa
     1021 ggtgcacact ttttatgttt aacaaaaacg aaatattaaa aaaacttatt gaagaatatg
     1081 atgttaaaac aactacagat attcaagata tgctaaaaga tttatttgcg agtacaattc
     1141 atcaaatgtt agaagcggag cttgacgacc atttaggtta tgatagatat ggcaataaga
     1201 ataaaaatac taaaaactct agaaatggat atagaaataa aaatgttaaa tcagattttg
     1261 gagaaataac tttaaatatt ccaagagacc gaaatggaga ttttgaacca aaggtaatta
     1321 gtaattataa aaatgatata tcaggaatag agaaccaagt gattggaatg tactcaaaag
     1381 gcatgtctac tcgagatata tcaagccacc ttaagagtat ttacggaatt gatatatctc
     1441 attctctagt agctaaaatt actgatagag ttctaccttt agctcatgaa tggcaaaaca
     1501 gacctcttga tatggtttat ccaattgtat ttatggatgc tatacattat aaagttagaa
     1561 gtgaaggtag aataataaac aaagctgcat atatagccat aggtgttaac ttagaaggta
     1621 taaaggaggt cttaggaata tggattggag aaaatgaaag ttcaaagtat tggctaaagg
     1681 tgcttacaga tgtcaaaaac agaggagtta aggatatatt aataacttct gtagatggat
     1741 tagttggatt ttctgatgca attaaagcag tatttcctga tactgaaata caaagatgtg
     1801 ttgtacatca aattagaaat actttaaatt atgtatcata taaaaataga aaagaatgtg
     1861 caaaagatta aaagaaagta tatgctgcaa ttacagagga agtagcatta agtgagctat
     1921 cgctcttaga ggaaaaatgg ggtaatcaat atgcgatagc attaagaagt tggcgaagca
     1981 attgaaatga gctagc
//
LOCUS       DAELSY010000288         1983 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.290, whole genome shotgun sequence.
ACCESSION   DAELSY010000288 DAELSY010000000
VERSION     DAELSY010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.290"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            178..294
                     /gene="rrf"
                     /locus_tag="KPG09_003802"
     rRNA            178..294
                     /gene="rrf"
                     /locus_tag="KPG09_003802"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            301..375
                     /locus_tag="KPG09_003803"
     tRNA            301..375
                     /locus_tag="KPG09_003803"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:333..335,aa:Asn,seq:gtt)
     gene            380..454
                     /locus_tag="KPG09_003804"
     tRNA            380..454
                     /locus_tag="KPG09_003804"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:413..415,aa:Glu,seq:ttc)
     gene            460..535
                     /locus_tag="KPG09_003805"
     tRNA            460..535
                     /locus_tag="KPG09_003805"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:493..495,aa:Val,seq:tac)
     gene            541..617
                     /locus_tag="KPG09_003806"
     tRNA            541..617
                     /locus_tag="KPG09_003806"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:575..577,aa:Asp,seq:gtc)
     gene            628..702
                     /locus_tag="KPG09_003807"
     tRNA            628..702
                     /locus_tag="KPG09_003807"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:660..662,aa:Thr,seq:tgt)
     gene            717..801
                     /locus_tag="KPG09_003808"
     tRNA            717..801
                     /locus_tag="KPG09_003808"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:751..753,aa:Tyr,seq:gta)
     gene            811..884
                     /locus_tag="KPG09_003809"
     tRNA            811..884
                     /locus_tag="KPG09_003809"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:843..845,aa:Gly,seq:tcc)
     gene            895..971
                     /locus_tag="KPG09_003810"
     tRNA            895..971
                     /locus_tag="KPG09_003810"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:929..931,aa:Arg,seq:tct)
     gene            981..1056
                     /locus_tag="KPG09_003811"
     tRNA            981..1056
                     /locus_tag="KPG09_003811"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1014..1016,aa:Gln,seq:ttg)
     gene            1068..1143
                     /locus_tag="KPG09_003812"
     tRNA            1068..1143
                     /locus_tag="KPG09_003812"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1101..1103,aa:Lys,seq:ttt)
     gene            1146..1234
                     /locus_tag="KPG09_003813"
     tRNA            1146..1234
                     /locus_tag="KPG09_003813"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1180..1182,aa:Ser,seq:tga)
     gene            1252..1342
                     /locus_tag="KPG09_003814"
     tRNA            1252..1342
                     /locus_tag="KPG09_003814"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1286..1288,aa:Ser,seq:gct)
     gene            1351..1427
                     /locus_tag="KPG09_003815"
     tRNA            1351..1427
                     /locus_tag="KPG09_003815"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1385..1387,aa:Pro,seq:tgg)
     gene            1513..1588
                     /locus_tag="KPG09_003816"
     tRNA            1513..1588
                     /locus_tag="KPG09_003816"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1546..1548,aa:Trp,seq:cca)
     gene            1649..1725
                     /locus_tag="KPG09_003817"
     tRNA            1649..1725
                     /locus_tag="KPG09_003817"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1683..1685,aa:Pro,seq:tgg)
     gene            1732..1808
                     /locus_tag="KPG09_003818"
     tRNA            1732..1808
                     /locus_tag="KPG09_003818"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1766..1768,aa:Ile,seq:gat)
     gene            1812..1887
                     /locus_tag="KPG09_003819"
     tRNA            1812..1887
                     /locus_tag="KPG09_003819"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1845..1847,aa:Phe,seq:gaa)
     gene            1895..1971
                     /locus_tag="KPG09_003820"
     tRNA            1895..1971
                     /locus_tag="KPG09_003820"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1929..1931,aa:Met,seq:cat)
BASE COUNT          501 a          449 c          535 g          498 t
ORIGIN      
        1 aatgtattta gcttatcgat actaataggt cgaggacttg accaatatta tttgatgtta
       61 ttcattaaga tatatgtgca gtttttagag tgttaactct aaaaaaaaat aaaaaaagta
      121 ttgacaaaaa tagtaaaaaa tgttaatatt atacttgtcc tagcaacaag aaaaaaatgt
      181 ggttataata gcagagaaga tacacctgtt tccattccga acacagaagt taagttctct
      241 agcgctgatg gtacttggtg ggaaactgcc tgggagagta gggcatagcc acgtgaatgt
      301 tccaaggtag ctcaacggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc
      361 ccacccttgg agccattatg gctcgttggt caagaggtta agacaccgcc ctttcacggc
      421 ggtaacaggg gttcaattcc cctacgagtc accatttgtg ggatcatagc tcagctggga
      481 gagcacctgc cttacaagca gggggtcaca ggttcgagcc ctgttggtcc caccatttgc
      541 ggcctggtag ttcagttggt tagaatgcca gcctgtcacg ctggaggtcg agggttcgag
      601 ccccttccag gtcgccaaaa taaaaatgct ggtgtggctc aacggtagag cagctgactt
      661 gtaatcagca ggttgtaggt tcgattccta tcaccagctc catgtaaaac taatacggag
      721 gatttcccga gcggccaaag ggggcagact gtaaatctgt tgtcatcgac ttcggtggtt
      781 cgaatccacc atcctccacc aattaaatat gcgggtgtag ctcaatggta gagttctggc
      841 cttccaagcc agctgtgagg gttcgatccc cttcacccgc tccaaagaaa gcgtgggtgc
      901 atagctcagc tggatagagc aacgcccttc taaggcgtgt gtccggggtt cgaatccctg
      961 tgcgctcacc acatatatat tggggattag ccaagtcggt aaggcacatg actttgactc
     1021 atgtatgcgt aggttcgagt cctgcatccc cagccaaata aaaatatggt ctattagctc
     1081 agtcggtaga gcacctgact tttaatcagg gtgtcccgcg ttcgagtcgc ggatagatca
     1141 ccaatggaga ggtgtccgag tggtttaagg agctggtctt gaaaaccagt gacttcgaaa
     1201 ggggccgtgg gttcgaatcc caccctctcc gccaaaaaaa tgactgaatt tggagaagta
     1261 ctcaagtggc tcaagaggat cccctgctaa gggattaggc tgggtaaccg gtgcgagggt
     1321 tcgaatccct ccttctccgc cattcaaaaa cgaggtgtag cgcagtttgg tagcgcacat
     1381 ggtttgggac catggggccg ggggttcgag tcccttcacc tcgaccaact taagctttta
     1441 gttcagttgg ttagagtgcc caactcatgg ctagtaattc tagggtttga gtccatgaag
     1501 gttattcttc ataggggtgt agctcagttg gtagagcgtc ggtctccaaa accgtgcgcc
     1561 gggggttcga gtccctccac ccctgccaaa taaaaaggac ttgaaaaaag ttggatttta
     1621 gagtagtata agatagtaaa taagatttcg aggtgtagcg cagtttggta gcgcacatgg
     1681 tttgggacca tggggccggg ggttcgagtc ccttcacctc gaccaagaaa agggcctata
     1741 gctcaggtgg ttagagcgca cgcctgataa gcgtgaggtc gctggttcga gtccagctag
     1801 gcccaccatt tgcccagata gctcagtcgg tagagcaggg gactgaaaat ccccgtgtcg
     1861 gtggttcgat tccgcctctg ggcaccagga atacggcggt atagcttagt tggctagagc
     1921 gttcggttca tacccgaaag gtcataggtt cgactcctat taccgctacc atctaactat
     1981 atc
//
LOCUS       DAELSY010000289         1961 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.291, whole genome shotgun sequence.
ACCESSION   DAELSY010000289 DAELSY010000000
VERSION     DAELSY010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.291"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            128..742
                     /locus_tag="KPG09_003821"
     CDS             128..742
                     /locus_tag="KPG09_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG0968353.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            760..1122
                     /locus_tag="KPG09_003822"
     CDS             760..1122
                     /locus_tag="KPG09_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0968354.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYKGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1228..1725
                     /locus_tag="KPG09_003823"
     CDS             1228..1725
                     /locus_tag="KPG09_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBG0968355.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          746 a          223 c          328 g          664 t
ORIGIN      
        1 tatttatgct agtactaaag acttgcaaac gttttttgac aagtctttat tttgtctaaa
       61 ttaaacaaac ttttttacaa ataaatacat aaaatttaaa ctaatctatt tattggagga
      121 gataaaattg gaagaagtaa ttgcaaaaat tattactata gaaaatggat tcaaagaaat
      181 tgaaaacatt gctaaaaaaa ttgtaaaaaa taatgactca aaaaagtgtt attatctatc
      241 aatctactta tataggtcag aatattatca agtaagagaa ttggctgtgt ttatacttgg
      301 ttttatatca gtaactattt ctgaagcttt gttattttta aaagacaatg tgagtaaaga
      361 tgaaaactgg agagtacaag aaatactagc taaatcattt gattattatt gcagtgaagt
      421 aggatatgaa aaggcacttc ctgttataaa agaatggctt aattctgata gtccaaacat
      481 aaaaagggct gtgacagaag gtcttagaat ttggactagt agagactatt ttaaatctaa
      541 tcctgacgtt gctatatcac tactttctag tttaaaagaa gatgatagcg aatacttgag
      601 aaagtctgta ggtaatgctc ttagagatat aagtaaaaaa tatccagatt tagtaaaaaa
      661 agaattagat tcatgggata tatcaaataa aaaagttgag tttgtatata agcttgcttc
      721 aaaatgtatc ttggcaaagt aaaatggagg tgaacttatt tgaaacttcg tgatgagtat
      781 acttgcccat tagaactaac acatgatatg ataaaaggca aatggaaatc tattatatta
      841 tggagattac gcataggacc aacctcctta tcaaaacttg aaaaggaaat caatggtatt
      901 aatcaaaaga tgttacttga acaacttaaa gaattaattg aatttggtct tgtagataaa
      961 aagacatata aaggttaccc acttaaagtt gaatactcac ttacacctat tcgagggaaa
     1021 caaatattgg atgctttggc tataatgcaa gatataggaa ttagctttat gttggataat
     1081 ggtatggaag atattcttaa ggaaaaaggt gttttaaaat aaatagatac ccacaaaaaa
     1141 gtaagtaatt taccattatt ttcaactggt atataattaa attatagata tattctatta
     1201 atttattata ataggaggta aattattatg aaagtaacta gtacaggtat agtaaatggt
     1261 gttatagaag ataaatacgg taaaagagga actcagttca atgaaggtgg tatgcctaca
     1321 tattcactac ctctaaagtt tgaagatgct ccaaagaata cagtatcctt tgctgtattt
     1381 ttagaagata aagactctgt tcctgtatgt gggtttgcat ggatacattg gttagctgta
     1441 aatataacaa aagatgagtt aaatgaaaat gaaagtgtaa cagctactga ttttgtacaa
     1501 gggactacta gttggcatgg caaactagga ggtcttgaca gattagaggc cagtgtatat
     1561 ggtggaatgg ctcctccaga cagacctcat gtctatgaaa tacatgtatt tgcacttgat
     1621 actatccttg accttgaaaa aggattctat atgaatgaat ttttcaaagc tattgaaggg
     1681 catgttttag atacatttac actaaaagga agctattcta attaattagg tttactatag
     1741 ctaatcatta attaaatgct acatttaatt gatgattagc ttaattgtta ttagaatatt
     1801 ttctgcactt tatcttaata tatattttag ttgactggta ttttaatttc tattagatta
     1861 ttatcaattt atataaattg attttagctt aaaacttact tatctatttc agtttctatt
     1921 agaattatta ttagactcat taaaataaaa gcattaatat a
//
LOCUS       DAELSY010000290         1936 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.292, whole genome shotgun sequence.
ACCESSION   DAELSY010000290 DAELSY010000000
VERSION     DAELSY010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.292"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            492..1652
                     /locus_tag="KPG09_003824"
     CDS             492..1652
                     /locus_tag="KPG09_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG0968356.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALVVTQILHMLA"
BASE COUNT          700 a          193 c          320 g          723 t
ORIGIN      
        1 tattagttaa atttaaattc atattgtttc ctcctaaata aatatattta tacaaattat
       61 aacataaata caaatgttct gaaaactaaa attaaaagaa tagaatctaa acatatttaa
      121 aaaaaaagta tataaaataa atttgattta tattatatag atttaaagta aaaatagttt
      181 tatttaataa ttaataaaaa taaatacaaa acaaaataaa taaataataa aatatttagt
      241 ttaattatct ttacaaataa aaattttagt aataaaataa ttgttgaaaa agattttaaa
      301 ttcagattat tcatttctaa agtaattaat gtgcaaatct ttattaatat atgtcaatag
      361 ttaggtatat atgtatttaa ttagcatgat atagataaaa gtatttattt taattgtcta
      421 ttatctgaaa aagtctttac ttagaaatca taaaaatcaa attaattagg aaaagaaagg
      481 gtgtgtataa tgtgattgag actggatttg tatattgtag tttcttggtt gtattcgtag
      541 gtgtaatttt ttttatggtt gataaattga aatggaaggt atttgatgtt attaatccaa
      601 tgcttttaat atacttaggt tcagctatac ttggatcatt tggtttattt gcacagaatg
      661 aagatgttgg aacgtatcaa aacataataa cagtgaactt ccttccatta atgttagtgt
      721 tcttgttaat tcaatgtgat ttaagaaaaa tattaaagat ggggcctaaa atgttattat
      781 catttttctg tgctacattt acatttatgc ttggatttgt tatagcattc ataattttta
      841 aaaattcaat gggtgcagat tcatggaaaa ttcttggggc agcatctggt tcatggatag
      901 gtggctcatc aaatatgatt gcagcagcta atgctctagg agttgcagaa gaaggactta
      961 gttatgcaat acttatgggt tctataggat atacagtatg gatgtctgtt tgtctaatgt
     1021 ctgtgaaatt tggacctaaa tttaacaaat ggacaaagtg cgatacaagt tttatagatg
     1081 aggttacatc aaaacttgaa aaagaaaata aagataatac ggcacctacg tttgtagaac
     1141 ttatgatttt actttctgtt gggtttggag ttgcgtcttt tgcacaaatt gtatccaaga
     1201 tacttccaac ttcaggtata cttaatgaaa ctatgtgggt agtacttgtt gcatctacag
     1261 tatcaatatt attgggtatg acaaaaatat caaagcttaa agggagtaca ctggtaggaa
     1321 atgtgtttat gtatttactc cttgcagcta caggtagtaa agcaaatttc tttggattgt
     1381 cagaagcacc tgtttacata atgtttggat tggtagtagt ccttgtacat gctatttcat
     1441 ttatagggct tgctaaacta tttaaacttg atttatttac ttgtgaagta ggaagtatag
     1501 ctaatatagg aggagtaact acagctccaa tgattgctgg ggttcacaat cctgtattaa
     1561 taccagtagg tgtacttatg gggcttctag gaaatattat agggacttat tgtgctcttg
     1621 tggtaactca aatattacat atgttagctt aataaaaata tggtataaaa ttaataattt
     1681 aaataagaaa agtcatttca atttaagcag aagcttttac tgaaatggtt ttttttgttt
     1741 gacaagataa tctatttcaa gaagatgttt tgacatattt agtgtataat attgtaaaat
     1801 ttactaaaaa cgagatatat ttaattttta tattgaatcg aacgtctgta tgatgtataa
     1861 taaatataca gaacgaatgt ttgcagaacg ggtgtttttt gaatttattt taataattta
     1921 tgaatatata tcagaa
//
LOCUS       DAELSY010000291         1913 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.293, whole genome shotgun sequence.
ACCESSION   DAELSY010000291 DAELSY010000000
VERSION     DAELSY010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.293"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    379..642
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            793..1344
                     /locus_tag="KPG09_003825"
     CDS             793..1344
                     /locus_tag="KPG09_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBG0968357.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          784 a          183 c          271 g          675 t
ORIGIN      
        1 taattaaaat ggagtaatat tctgtttcaa aggaggcata taaatacagg aatgagtatt
       61 aaatgatggt taattaagta aatttattga taaatgtact taattaactt tgaagaattt
      121 aattcctcat gaatcaaaat ataaaattac atcaacagtt attcacaaaa ttaatataag
      181 aagaattaga gccaaagagc agactattag accattaaag taattattca tagaattttg
      241 tatatgaaaa gaaatgtata gtataattta ctttttttat aaaaaataga ttacaaaata
      301 tattttttat aaaaaggtat tgacattttt tagaaaaaag aatatcatta ttttaacaac
      361 aaaattcaat acaataaatt aaatcctatg acaaggaaga gtagttaaat ttaggttcag
      421 tatagagagc tgaggatggt ggaatcttag caagaaaaaa tttgatgaat ggcccttaga
      481 ggaataaatt gaaatcatag ttttgagagt agactttatc gtcagtcgca cgttatagcg
      541 aataaggtat gtaagtacct gataatgaga tgtatgaaaa ttgaatttta tattaatttt
      601 tatataattt aggtggcaac gcggataatc tccgtcctat tagtttagga cggagttttt
      661 tattttactg aaaatttaaa agatagaagg tggtgagaaa gatgttatga atttcaaaag
      721 ctaaagatga tgatatagta ggttggcaaa aaaataaatt gaaaattaaa aaaataggag
      781 gaagtacaaa aaatgaaaac aaatacaaaa aaattaactt taaatgcaat acttttagcg
      841 atgggattat taatacatca aatcacacca gctataggat taccaatgaa gccagatgta
      901 ccactagcaa tgttatttgt aatattggtt ttaaatagag atgattataa aacttgtttg
      961 atagcgggta tagtaacagg aatatttaca gcacttacat caagttttcc gggaggtcaa
     1021 attcctaatg taatagataa aactcttaca gcaaatatag tatttttatt aatgaatatt
     1081 agttataata tgccttttat aaaaaaatta gctaagaaaa cacaagatac tgtagtagtt
     1141 gcaataataa tgccaatagg aactcttgta agtggaacta tctttttatt agcagctcaa
     1201 gctatagttg gtcttccagg agcttcattt acagctttat tcttagcagt agtattacca
     1261 gctgttttaa taaatctagt tgcaggaata tttttattca agattgttag tctatcaata
     1321 agaagagtta gttatcaagc ttaactacga aataatattt tactttataa agcattataa
     1381 taactaaata acataagaat aaagtagatt ataactattt tattcttatg ttgtttaaag
     1441 atttggtatt atatatttat ttttaataaa atatttatat agtaccaatt acacttaatt
     1501 taacagataa attatcatac ctatataata ttcttatatg tatctcattt gtagttatta
     1561 aatataagat atatttaatt gacttttcaa gataaatttt tatattaatc tcctctgatt
     1621 tctcattttt tgaatacact aaaatttcca attaaatcaa ttatatattt atagagtttt
     1681 aaaaatatag taatgtatat attttttgtg gaaaacttgc ttaaatttag caaaaaaaat
     1741 agataattca tagtataaaa taaagtttag taaaaaattt taatagaact ataaaagtct
     1801 gttttagtaa aagatttaaa attctaatat taagtgaaat tttgggtgaa ttaaataagc
     1861 gaaactataa tagaaataga atatatggaa aaatacatct ctatgcttaa aaa
//
LOCUS       DAELSY010000292         1715 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.294, whole genome shotgun sequence.
ACCESSION   DAELSY010000292 DAELSY010000000
VERSION     DAELSY010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.294"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            219..1649
                     /locus_tag="KPG09_003826"
     CDS             219..1649
                     /locus_tag="KPG09_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG0968358.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKALKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEDQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEEICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
BASE COUNT          803 a          151 c          304 g          457 t
ORIGIN      
        1 ataaatgagt aataaatata aatagtgtat actaatggga tggaaaatgt ccactattaa
       61 ctttattacc ccacatatat aagataaaat tattaatata aaatataatc tatcgacaat
      121 gaatagactt ttaaataaac ttaatataaa attaattaaa taagtgctta aaaacgattt
      181 actttaatga tagaatcata ttaaaggagg ataattttat ggatataaag aataaaatta
      241 aatctatatt tttaccagaa aaaaatgact atttagatga tgaagaattt gagtatgata
      301 tagaaaataa tgaagaagat ttaaaggaag ataacaaagt acatttaaat gatttaagca
      361 gagtaaaata tgattacaga gagttaaaag acatagaaaa ccaaacagaa gagatgtgtc
      421 tgctagcagt aaagcaggat ggtactataa ttgagtttgt aaaggacaag acttataaag
      481 tatgtatgga agcagtaaaa cagacatata aagctcttaa gtatataaca aatcaaaatg
      541 aggatatttg tatagaagca gtaaaacaga actacagagc tttgtactac ataaataata
      601 agacagaaaa tgtactaatt gaggctataa aaaatgcatc aacttatgat gtcatggaag
      661 tatttaaatt tgtggaagat caaacagaag atgtatgtct agcatttata gaaagggcat
      721 ctaaaaatga tgttgcagag atattaaaag gaataaaaga gcaaactcca gccatatgtt
      781 tagaagctgt aaaaaaagat ggaaaatctt tagcttatgt aaaggaacaa agtaatagta
      841 tatgtttaga agctgtaaaa gaaaactaca gtgcactatc ttgtgtaaaa gagcaaacag
      901 aagaaatatg tatagaagca gtaaaacaga atgactttgc attgtattat gtaaatgaac
      961 agacagaaaa aatatgtatg gaagcagtaa aaagaagtta tatggctcta cagtatgtaa
     1021 ataagcaaac agaagagatt tgtttggaag cagtaagaat tgatggaaga gcattacagt
     1081 acgtaaaaga gcaaacagaa gaaatttgct tggagtcagt tagacaaaat ggaaaagtat
     1141 tgcaatatgt aaaaaaacag actgaaaata tatgtataga agcagtaaga ggttcatttg
     1201 aagaacttga aataaaagaa atattaagtt atgtaaagat accaacagaa agaatatttg
     1261 tagaagcagt aaagcaaaat ggaaaaattc ttaaatatgt agaaaatcag acagaattaa
     1321 tatgtttaga ggcagtaaga gaaaactata atgctttagc ttatgtaaag gaacaaacag
     1381 aagagatttg tttagaagca gttaatcaaa gttatgaagc gcttaaatat gtgaaagaac
     1441 aaacagaaga agtttgtcta aaagcagtaa aacaagatta tagaatgttg aaatatgtaa
     1501 ataatcaaac agaaaaaata tgtttagaag cagtaaagca aaattataga gctttagaat
     1561 ttgttgataa tcaaacagaa aaagtttgtc ttgaagcagt aaaacagaat agaaaagcac
     1621 tacagtatgt aaaacaaaaa caaagttaaa aggctaaaat atatagaaaa tatatgcaaa
     1681 taaataaaat ttgatatttt gtaataaata aaatg
//
LOCUS       DAELSY010000293         1662 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.295, whole genome shotgun sequence.
ACCESSION   DAELSY010000293 DAELSY010000000
VERSION     DAELSY010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.295"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>1662
                     /locus_tag="KPG09_003827"
     rRNA            <1..>1662
                     /locus_tag="KPG09_003827"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          477 a          347 c          492 g          346 t
ORIGIN      
        1 gggacacgtg aaaccctgtg ggaagcagga gggaccaccc tccaaggcta aatactacct
       61 agtgaccgat agcgtatagt accgtgaggg aaaggtgaaa agaaccccgg gaggggagtg
      121 aaatagaacc tgaaaccctg cacttacaag ctgtggaagc acatttcttg tgtgaccgcg
      181 tactttttgt agaacgggcc aacgagttac gttaagtagc aaggttaagc acttaaggtg
      241 cggagccgta gcgaaagcga gttttaactg agcgttcagt tacttgacgt agacccgaaa
      301 ccgggcgacc tacccatgag caggatgaag cgaaagtaaa atttcgtgga ggtccgaacc
      361 cacgagcgtt gaaaagctcg gggatgactt gtgggtagcg gtgaaattcc aatcgagccc
      421 ggagatagct ggttctcccc gaaatagctt tagggctagc ctcaaggtga gagatacgga
      481 ggtagagcac tgaatgtcct agggggtatt gcacctaccg aagactatca aactccgaat
      541 gccgtcatct tatacttggg agtcagactg tgggtgataa gattcatagt cgaaagggca
      601 acagcccaga tcgtcagcta aggtccctaa atgtaagtta agtggtaaag gatgtgggat
      661 tgcacagaca accaggatgt tggcttagaa gcagccactc attcaaagag tgcgtaatag
      721 ctcactggtc gagtgatcct gcgccgaaga tttccggggc taaaacttac taccgaagct
      781 acggcatcag taatgatggg taggggagct tcccatacgg gttgaagcat gaccgtaagg
      841 acatgtggac agtatgggag tgagaatgtt ggcatgagta gcgagatgtg ggtgagaatc
      901 ccacaggccg taaacccaag gtttccaggg gaaggttcgt ccgccctggg ttagtcggga
      961 cctaagctga ggccgaaagg cgtaggtgat ggacaacagg ttgatattcc tgtactaccg
     1021 ataaccgttt gagagaaggg atgacacagt aggataagct aagcacactg ttggttatgt
     1081 gtgcccaagc attgaggcag tcaaagtagg caaatccgct ttgataatgc tgggatgtga
     1141 tggggagcga aatttagtag cgaagtagct gatttcacac tgtcaagaaa agtctctatc
     1201 gaggttaaag gtacccgtac cgcaaaccga cacaggtggg tgaggagagt atcctaaggc
     1261 cagcgagaga actgttgtta aggaactcgg caaaatgacc ccgtaactta gggataaggg
     1321 gtgccaccat caggtggccg cagagaatag gcccaagcga ctgtttacca aaaacatagg
     1381 tttctgctaa gtcgcaagac gatgtatagg agctgacgcc tgcccggtgc tggaaggtta
     1441 aggggatctg ttagagcaat cgaagcagtg aacttaagcc ccagtaaacg gcggccgtaa
     1501 ctataacggt cctaaggtag cgaaattcct tgtcgggtaa gttccgaccc gcacgaaagg
     1561 cgtaacgatt tgggcactgt ctcaacaaca gactcggtga aattgtaatt ccggtgaaga
     1621 tgccggatac ctgcgacagg acggaaagac cccatggagc tt
//
LOCUS       DAELSY010000294         1658 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.296, whole genome shotgun sequence.
ACCESSION   DAELSY010000294 DAELSY010000000
VERSION     DAELSY010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.296"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1277)
                     /locus_tag="KPG09_003828"
     CDS             complement(<1..1277)
                     /locus_tag="KPG09_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBG0968359.1"
                     /translation="MIFSSLIFLFLFFPVTLICYYLANPKYKNVMLLLASIAFYAWGE
                     PKYIFLMILSIIVNYIFGIKVSGSKNKKMWVVLSVIYNISILAVFKYSNFFIDNINFL
                     FNTDITIPEVKLPLGISFFTFQIMSYVIDVYRNDAKVQRKLANLALYISLFPQLVAGP
                     IVRYQTVADSIEYREHNWDKFTQGVNRFVVGLGKKVILANQLGVIADGVFNKSAGDLS
                     RLEVWLGIVCYTLQIFYDFSGYSDMAIGLGKMFGFEFLENFNYPYISQTVSEFWRRWH
                     ISLGTWFKDYVYFPLGGSRVSKAKLYRNLFVVWFLTGMWHGASWTFIIWGLYYGILIS
                     VEKAFLEKFLYKMPRIVRHIYLLLIVMIGWVFFRVDDLPAAIKFIGIMFGAGNYPVIS
                     DAFNVFIIEYWYIIVGAILLSTPIVKVVKSLLI"
BASE COUNT          626 a          266 c          191 g          575 t
ORIGIN      
        1 ttaattaata atgattttac aacttttaca ataggagttg atagaaggat tgccccaaca
       61 attatatacc aatattcaat tataaataca ttgaatgcat cactgataac cggataattt
      121 ccagcaccaa acataatacc tataaattta atagctgctg gtaaatcatc aactctaaaa
      181 aatacccatc caatcatgac tataagaagt agatatatgt gtcttacaat tcttggcatc
      241 ttatatagaa atttttctaa gaatgctttt tctactgata ttaatattcc ataatataga
      301 ccccatataa taaaagtcca actagctcca tgccacatcc cggttaaaaa ccatactaca
      361 aatagatttc tatatagttt tgccttagag actctacttc cacctaatgg gaagtacaca
      421 tagtctttaa accaagtacc taaagatata tgccatcttc tccaaaactc agatactgtt
      481 tgagagatat atggatagtt gaaattttct aaaaactcaa atccaaacat cttacctaaa
      541 ccaatggcca tatcactata gccactaaag tcatagaata tttgaagtgt atagcaaact
      601 atacctagcc atacctcaag tcttgataaa tctccagctg acttattaaa tacaccatct
      661 gcgattacac ctaattgatt tgctagaatt actttttttc caagaccaac aacaaatctg
      721 tttacacctt gagtaaattt gtcccaattg tgttctctat actcaatgct atctgcaaca
      781 gtctgatatc taacaatagg acctgctaca agctgaggga ataaacttat gtataatgct
      841 aaattagcta attttctttg aactttagca tcatttctat atacatctat tacatagctc
      901 attatttgga atgtgaaaaa tgagatacct aaaggtaatt taacctcagg tatagttata
      961 tctgtattga ataaaaaatt aatattatct ataaaaaaat tgctatactt aaatacagca
     1021 agaatagata tattgtaaat aacagaaagc acaacccaca tttttttatt tttactgcca
     1081 gaaactttaa taccaaatat atagtttact attattgata gtatcatcaa aaaaatatat
     1141 tttggttctc cccatgcgta gaatgcaata cttgctagaa gtaacataac atttttatac
     1201 ttaggatttg ccaagtaata gcaaatgagt gtgactggaa agaataaaaa taaaaatatt
     1261 aaactactga atatcatatt agactccttt cttaaccaaa tcaattatat ctttatctaa
     1321 aatatatagc aaagtaagta ttttcaagtt ttaagtactt aattgtatga atttatatgt
     1381 gtttttacaa gtttaattaa tctatatgta aattttgact tttcatataa tttttttaaa
     1441 atgatgtaat attaaaatga attaataaag ctattataat aaagaaaaat caaggacata
     1501 ttacttaact atatgtgttt acctttgatt gtgcctctat acagtctatt aagatagatg
     1561 ttgtttatat ctataactgt aaaaagaact ctttattaag tattagaatc agaatacttt
     1621 cagatatgct tttatcgcaa gtttgtttta aatgacaa
//
LOCUS       DAELSY010000295         1646 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.297, whole genome shotgun sequence.
ACCESSION   DAELSY010000295 DAELSY010000000
VERSION     DAELSY010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.297"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1514
                     /locus_tag="KPG09_003829"
     CDS             <1..1514
                     /locus_tag="KPG09_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase C80"
                     /protein_id="HBG0968360.1"
                     /translation="SKAITGWQTIDGKKYYFNPNNAIAATHLCTINNDKYYFSYDGIL
                     QNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQNKFL
                     TLNGKKYYFDNDSKAVTGWQTIDSKKYYFNLNTAVAVTGWQTIDGEKYYFNLNTAEAA
                     TGWQTIDGKRYYFNTNTYIASTGYTIINGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     THNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAVTGW
                     QTINGKKYYFNTNTYIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPANTDA
                     NNIEGQAIRYQNRFLYLHDNIYYFGNDSKAATGWATIDGNRYYFEPNTAMGANGYKTI
                     DNKNFYFRNGLPQIGVFKGPNGFEYFAPANTDANNIDGQAIRYQNRFLHLLGKIYYFG
                     NNSKAVTGWQTINSKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
BASE COUNT          588 a          217 c          274 g          567 t
ORIGIN      
        1 actcaaaagc aattactgga tggcaaacca ttgatggtaa aaaatattac tttaatccta
       61 ataatgctat tgctgcgact catctatgca ctataaataa cgacaagtat tactttagtt
      121 atgatggaat tcttcaaaat ggatatatta ctattgaaag aaataatttc tattttgatg
      181 ctaataatga atctaaaatg gtaacaggag tatttaaagg acctaatgga tttgagtatt
      241 ttgcacctgc taatactcat aataataaca tagaaggtca ggctatagtt taccagaata
      301 aattcttaac tttgaatggc aaaaaatatt attttgataa tgactcaaaa gcagttactg
      361 gatggcaaac tattgatagt aaaaaatatt actttaatct taacactgct gttgcagtta
      421 ctggatggca aactattgat ggtgaaaaat attactttaa tcttaacact gctgaagcag
      481 ctactggatg gcaaactatt gatggtaaaa gatactactt taatactaac acttatatag
      541 cttcaactgg ttatacgatt attaatggta aacattttta ttttaatact gatggtatta
      601 tgcagatagg agtgtttaaa ggacctgatg gatttgaata ctttgcacct gctaatactc
      661 ataataataa catagaaggt caagctatac tttaccaaaa taaattctta actttgaatg
      721 gtaaaaaata ttactttggt agtgactcaa aagcagttac cggattgcga actattgatg
      781 gtaaaaaata ttactttaat actaacactg ctgttgcagt tactggatgg caaactatta
      841 atggtaaaaa atactacttt aatactaaca cttatatagc ttcaactggt tatacaatta
      901 ttagtggtaa acatttttat tttaatactg atggtattat gcagatagga gtgtttaaag
      961 gacctgatgg atttgaatac tttgcacctg ctaatacgga tgctaacaac atagaaggtc
     1021 aagctatacg ttatcaaaat agattcctat atttacatga caatatatat tactttggca
     1081 atgattcaaa agcggctact ggttgggcaa ctattgatgg taatagatat tacttcgagc
     1141 ctaatacagc tatgggtgcg aatggttata aaactattga taataaaaat ttttacttta
     1201 gaaatggttt acctcagata ggagtgttta aaggacctaa tggatttgaa tactttgcac
     1261 ctgctaatac ggatgctaac aatatagatg gtcaagctat acgttatcaa aatagattcc
     1321 tacatttact tggaaaaata tattactttg gtaataactc aaaagcagtt actggatggc
     1381 aaactattaa tagtaaagta tattacttta tgcctgatac tgctatggct gcagctggtg
     1441 gacttttcga gattgatggt gttatatatt tctttggtgt tgatggagta aaagcccctg
     1501 ggatatatgg ctaaaatata tgtttgacaa aaaattattc ctgtgatact cagaaattat
     1561 ttttatataa taaatattga gatttaatta aaagtcatgt gttattgtaa tacatgactt
     1621 ttagttaaaa tttttctatc atttaa
//
LOCUS       DAELSY010000296         1559 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.298, whole genome shotgun sequence.
ACCESSION   DAELSY010000296 DAELSY010000000
VERSION     DAELSY010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.298"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            99..1535
                     /locus_tag="KPG09_003830"
     CDS             99..1535
                     /locus_tag="KPG09_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBG0968361.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPIVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT          641 a          162 c          266 g          490 t
ORIGIN      
        1 tataatttgc aattttacat aagtttgtta agtacataac taatttaaga atatgtattt
       61 atatattaaa aatttattat gagggggaca attttattat gaaattctat aaaagaatat
      121 taacattgac tttaagtatt tcttttgtat ttgcaaatat tcaattagta gatgcaatat
      181 catctgtaga aaaaatacaa ggaaaaaata agtatgaaat agcaggcaaa atagcagata
      241 aaaatgcata taaaactgct atattaatta atactagtaa ctctattgca gatggattaa
      301 gtgcaagtgg actagcagga gctttaaatg cccctatact attaactgaa aaaaatacta
      361 taccaactga gacatctgca agattaaaaa atgtatcaaa agtttatata attggaggaa
      421 catattctat tagtacatct gtcgaaaatt cattaaaatc taagaaaatg aaagttgtaa
      481 gaattaaggg aaatgacaga ataaaaacaa gttataatgt tgcaaaagaa ataaattcaa
      541 taaaaaaagt caatactgtt atgctaacca atgcatataa aggagaagca gatgctataa
      601 gtattgcatc tgtagcagca agagataaag caccaataat tcttactaat ggacaaagta
      661 tacctttttc aactagtggt ttaaagtctt atgtaatagg aggaacagct tcaatgagta
      721 ccactttagt aaacaatact aaatctacta gactaggagg gagtactcgt tttgaaacta
      781 ataaagcaat aataaataag ttttataagg atgctagaga gttttatata gctggagctt
      841 atgaattaac aaatgcactt gtaggatctt ctttatcaaa acatggtcca atagttttag
      901 ttaatgatgg tagcaataaa agcattttaa aaaatgctaa gaagataact tctataggat
      961 atatagattc aaatatagta caacagtgtt taaatataac aaatggaatt ggagatatta
     1021 atactggggt tgtaaaaaat gttaaaccta ctacaaagac tataaaagat ggaatgtata
     1081 aggttggaaa agatatatca gcaggtgaat acttaattac ttcaaactca ggaagttatg
     1141 cttcttatta tgaagttaca agtgatagta caggtaatgc tgatagtata ctttcaaatg
     1201 atatttttag tgggactaga tatatcacct taaagaatgg tcaatatatt aaaattgagg
     1261 attcaacaat gacattagca aagtatgcta aagcccagaa ggccaagaat ggtaagtttg
     1321 gaaatggtat gtataaaatt ggattagaaa ttcctgctgg agaatatatt ataatgtcaa
     1381 attcttctga tgcatattat gaagttagaa atgatagctt aggtaatgca gagggaatag
     1441 ttactaatga tactttttca ggaagaagat atataacggt tgaggaaggt caatatttaa
     1501 tattaaatga ttgttattta attgaaaatg aatagatgta attgtattta atagtaaat
//
LOCUS       DAELSY010000297         1540 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.299, whole genome shotgun sequence.
ACCESSION   DAELSY010000297 DAELSY010000000
VERSION     DAELSY010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.299"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(403..894)
                     /locus_tag="KPG09_003831"
     CDS             complement(403..894)
                     /locus_tag="KPG09_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBG0968362.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDENIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT          585 a          195 c          133 g          627 t
ORIGIN      
        1 atttttctgt ttttaattaa attaaagtat ctttgtttct gaaatttttt aagtttatga
       61 agctataatt taatatatat ttattttatt tgtgagtcta tttttatata taatgaaaaa
      121 tgtgataagt atccaaaaga attactggat ataataagat agggtattta gccttttaag
      181 ataaataaaa atatctgcaa ctatatattg caaaaattta aaactaattt atatatgcta
      241 atataaatat ttataaagac ataagacaaa tttacctaca gatttaaatt taacttatat
      301 attttaaaag gctaatataa tatgtatttt cttctataca atcaaaaggc ttcatatcta
      361 agatatgtta tgacactaat attcacaatt ttttagtaat tatcacatta taaaaaatat
      421 tacagctagt ccatcatcag atgcttcttg tataaacttt ttaagctcaa ttagatttgc
      481 ccatacatat tcaaaaaaat catcttcact ctcatcattc ataacaggat aaatattatt
      541 ttctaacatc tcattaatat taaatctttt tctaaatgct tgttcttcca aactgttcat
      601 tgcttttgat agttttttta catcatttgc tgtaatcaac atgacaggaa attctccatc
      661 aatttccata agtatgtttc cacttagtac taatttgcta aatatattct catcatctcc
      721 accaaaagga caacctgtca aagtaaagtg aatggcatgc catgctttat caatattgat
      781 agtactattt tcgttggcat cttcaaaaac aaaatcttgt aaattttctt ctgcttgctg
      841 taatttaaaa acattctctt cacttatttt agtataacat cctatcatcc ccataattac
      901 attcctccta agtagcgttt ttttaattgt aacttataat taaacttagt tacaataaaa
      961 tatattgaga aaagtattat ttcatcttaa atttaaatac ctatattatc aactaaaatt
     1021 ctgtgtttat agcgtaagta ttattatttt ttcctaaaaa ttaaatgaat cagaagttct
     1081 ataggcaata ttgactataa ataagaacta ttacttttaa aaaaaattta agaaattata
     1141 ctgtagaatc atgctctact tattagcttc aaataaaaaa taaatcatgt tctcaaatta
     1201 aataataaat aacaataaat ttaaatttta aactataaga gtttctgaag tgttaataag
     1261 cataccaata attatatgtg taagaatttg atttaataga tatgaataat ttttacatta
     1321 attataaata tattaaccta ttattataaa ttctataaaa agaaaagtta tcttaaataa
     1381 aaaataagtg tatcttaaat tttaaatgat acacttattt ttatattgaa attatatttt
     1441 ttaatatcta ttcttaatta ggtattttta aattatctgt ttacattaaa tatctcttaa
     1501 ttacttatct ttcagctagc ttcttctatt aaatgtttct
//
LOCUS       DAELSY010000298         1484 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.300, whole genome shotgun sequence.
ACCESSION   DAELSY010000298 DAELSY010000000
VERSION     DAELSY010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.300"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(213..920)
                     /locus_tag="KPG09_003832"
     CDS             complement(213..920)
                     /locus_tag="KPG09_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG0968363.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT          467 a          262 c          134 g          621 t
ORIGIN      
        1 gcaatttaac tttaaaaaaa taaaaatacc caactgacct aagcaactcg attgggtata
       61 aataatctaa aaaatcattt ttgtataaac tctttattta acttacattt ttatttacct
      121 tctactttaa ttcaacttat atttctatgc ttcttatact ctcattcaac ttatatttct
      181 acttatctta tacttttata aattcatcac aattatttag ttctcgctat caaagaaatt
      241 gtattaaaat actttccatt ctctacttcc atcatttttt tgtcatttat agcatatgaa
      301 ttatcacaat ttatccaatc cttccaagct tcatcatgac aatttaaagc ttcacatttc
      361 tctacaatca cagactccga ttcagaccaa agtttattcc accatgtaat tgaatggaag
      421 ttcatattat cttgccaaaa tggaatgagt tcatctggta tacaatccac aaaatcttct
      481 tttaatccag gcattgccat agcaagtatt cccccctctt ttacaagagg tgatatatga
      541 ttgtctaaaa atccttcttt gtttccaaaa tagtgataag aatcgatact tatcaccgca
      601 tcaaagaaac cttctgcata aggtagttca tgtgcctctg cctgtattgg aaatatttta
      661 tcatccaatt taaactcttt aaatctttta tagttttcag ttggttctat ccataaatct
      721 gttgcaaata cagtagcatc aaattccttt gctaaaaata tcgaagatat acccttacca
      781 caacctaagt ctaaaatacg cattcccttt tctagtttta tcttacttgt taattcttcc
      841 aatattttta tacaattagg tcccatcata tttttcatga gaaagtcctt atcgtattta
      901 ttgctttttg taaatatcat tctatctttc ctttccctat cagttattat ttattaaatt
      961 ttatatgttt gattttttat atgcttctta tattgagcaa tctctttttt attcattctt
     1021 taacagacaa ttttaatgtg taattttatc cactctgtga tagtaacttt aatgaataat
     1081 cttatctaat atgtgataat taactttgat gggtaatttt atctactcta caataatcac
     1141 cttaatgagt atcttatcta ctctattata gttattttag tgagtaattt tatctactcc
     1201 acagtaatcg ctttaatgaa taatcttatc cactctatta tagttatttt agtgagtaat
     1261 tttatctact ctatagtaat tgctttaatg agtaatctta tccactctat tatagctatt
     1321 tttatgggca attttatcta ctctataata gtcactttaa tgagtaatca taatttttat
     1381 atcattttag atacttactc taataagaag tcattttcta atacttaata agttttaatt
     1441 acttccactt taacctatat actaattaac ttatttaatt tagt
//
LOCUS       DAELSY010000299         1482 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.301, whole genome shotgun sequence.
ACCESSION   DAELSY010000299 DAELSY010000000
VERSION     DAELSY010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.301"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(75..1367)
                     /gene="brnQ"
                     /locus_tag="KPG09_003833"
     CDS             complement(75..1367)
                     /gene="brnQ"
                     /locus_tag="KPG09_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG0968364.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFALSYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVDVPVV
                     KSLPLHSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT          529 a          277 c          185 g          491 t
ORIGIN      
        1 tttgtacaaa taactccttt tacttttagt taactcattt atttacaatt gattatattt
       61 taattaaaca aaacttagtt tgttccaagt gtattagatt gactctttga tggtataaaa
      121 ttaccaatta ttccagctat tataactggt accacccagt taaatcctaa tgaatgaaga
      181 ggtaaagatt ttactactgg aacatctact ccataactag atataactgt tagtatactt
      241 acaagcaatg acatatatac tgctcctttg tacacattat cattcttaat tttttcattg
      301 aagaaagcta aaattattaa aacaattgat ggtggataaa ctatacttag tataggagat
      361 gctatcttta ttatagttcc aacaccaaaa gatgctacta ctgcactgaa tacagataca
      421 gctaatacta ttttttcata actcaattta ccttttgtaa gtctagagaa atattgacct
      481 gtggcagatg taagacctat agatgtagtt aaacatgcta atgatactac tactgctaaa
      541 atcgctttcc caacatttcc aagtaatcct tcagttatat ttactattac ttgtgattgt
      601 actgcatctg ttccatacat tgttgataca gttgctccaa ggaaacataa tcctccataa
      661 actagagcta atcctccaca agcaagaacc cccgccttac caatcatttt taacttgtca
      721 ctaatagtag aatatccttt atcatttaaa gatactatta acactgatgc aagtgctatt
      781 gctgcaaaag catccattgt ctgatatcct tgacctatac cttctgcaaa tacattaggt
      841 atcataggtt tatctactat atctccaaga ggtgatataa taccttttat aattataaat
      901 gctagagcta ttaaaagagc tggagttaaa aactgtccaa ctatatctac gaccttagaa
      961 ggtcttattg ttagtacata tgaaagcgca aaaaaaagta tacagaataa taacactgga
     1021 attgaagttc caaataaagg cattataccc atttcataag ttgttgcacc tgttcttggt
     1081 atagctaaga atggacctat acataaaatg tctgcacaac ctataagaat ccctaacttc
     1141 ataccacatc ttttaaacat ttcaactacg tttccatcga atttagctgt agccattact
     1201 gctagaagtg ccaatccagc atcagcaaac gtaaatccta agaaggcaat taaccattct
     1261 ggacctgtaa ttattcccaa gtaaggtgga aatattaagt tacctgctcc aaagaacatt
     1321 gcaaataacg caaatccaaa tactataaca tccttggtct tacccattat aaaatccccc
     1381 tttttaacct tattaaatta ataaaatgtc tcgattgttt acgcttgcat aaataaatta
     1441 aattaaacaa taagaactta ttattaaatt aataaaatta aa
//
LOCUS       DAELSY010000300         1481 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.302, whole genome shotgun sequence.
ACCESSION   DAELSY010000300 DAELSY010000000
VERSION     DAELSY010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.302"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            131..1384
                     /locus_tag="KPG09_003834"
     CDS             131..1384
                     /locus_tag="KPG09_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBG0968365.1"
                     /translation="MLNKKNISSIIMTMILSLSTTVFVFADENTNVKGQDVTKYNEEM
                     VSKNLEDLSIEQLIDIRDKAIDKNLEGLRKFGEDSVKERKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVEYENSINDIMDPSGDFFKVTNQSYQINEFVLKNGKNYKEWSY
                     EYVEFLIEHLEDLCNFVDFDAKYVIEMVDPSIKPDLSDNQKKSLNNELIKMYSSNTLS
                     TNIKDEIENWENNLNINEKNSNEPLPRVSPSSYRQQALAYAKKHGYTNGYYGSTNRKN
                     NAPSPYYNFQKDGYGDCANFVSQCLHEAGVTFWTNNNPWYYYSTSNRSPSWAGAREFK
                     IHWMNRIAYQTLTVSNSLSFLRPATPVSILNSSGVATHTLISTDKHSNGYNFSYAAHS
                     DEGARTNLLKKLEGKKISYYKVYWE"
BASE COUNT          618 a          151 c          228 g          484 t
ORIGIN      
        1 aggattatat tatgatattt agtaaacgct cttttagaaa aaaacggtga taaacttgtt
       61 ttaaatttta gtagaatatg atttttgttt tggcaagtat taatacatat attaaagagg
      121 gaggtttaat atgttaaata aaaaaaatat aagttcaatt attatgacaa tgattttaag
      181 cttatcaact actgtatttg ttttcgctga tgaaaatact aatgtgaaag gacaagatgt
      241 aaccaaatat aatgaagaaa tggtctctaa aaacttagaa gatttaagta tagaacaatt
      301 aatagatatt agagataaag ctattgataa aaatttagaa ggtcttagaa aatttggaga
      361 agattcagtg aaagaacgta aagattcaat tttaaacttt gaatttggat tagtagcttt
      421 tgttaacaaa aattatagta acttaacaga aaaagagaaa ttaataagac aaatcaatat
      481 tgttgagtat gaaaattcta taaatgatat aatggatcct tcaggcgatt ttttcaaagt
      541 aacaaaccaa agttatcaga ttaatgaatt tgtattaaaa aatggaaaaa actataaaga
      601 gtggtcgtat gaatatgtag agtttttgat tgagcattta gaggatttat gtaattttgt
      661 agattttgat gcaaaatatg taatagaaat ggttgatcct tcaataaaac cagatctttc
      721 agataatcaa aagaaatctc taaataatga attaataaaa atgtattcaa gtaatactct
      781 aagtacaaat ataaaagatg aaatagaaaa ctgggaaaat aatttaaata ttaatgagaa
      841 aaattcaaat gaacctttac caagagtaag cccttctagt tatagacaac aagcattagc
      901 ttatgctaaa aaacatggct atactaatgg ttattatggt agtacaaata gaaaaaataa
      961 tgcaccttct ccatattata attttcaaaa agatggatat ggagattgtg ctaactttgt
     1021 ttcacaatgc ttacatgaag ctggagtaac attctggacg aataataatc catggtatta
     1081 ttattctact agtaatagat caccttcttg ggctggagca agagaattta aaatacattg
     1141 gatgaatcga attgcatatc aaacacttac tgtatcaaac tctttaagtt ttttaagacc
     1201 agcaacacct gtaagtatat taaatagtag tggagtagca actcatacac taatatctac
     1261 agataagcat tcaaatggat ataatttttc atatgctgca cattcagatg aaggtgcaag
     1321 aacaaatttg ttaaaaaaac ttgaaggaaa gaaaatttct tattacaaag tttattggga
     1381 ataaataaaa atttatactt ttaattaaat gaaaattgaa aagaataaat atctatttca
     1441 agatccaata agtatttagt tgaagcagct attaaatttt a
//
LOCUS       DAELSY010000301         1455 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.303, whole genome shotgun sequence.
ACCESSION   DAELSY010000301 DAELSY010000000
VERSION     DAELSY010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.303"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(204..1388)
                     /locus_tag="KPG09_003835"
     CDS             complement(204..1388)
                     /locus_tag="KPG09_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0968366.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
BASE COUNT          481 a          203 c          161 g          610 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttactt tatatattaa ttaccattaa tacattatat
      181 ctaattacta ttcatctaaa ttattatgcc attattttat atacaaattc ttttaatttg
      241 tctaatccag ataaaaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttct tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa tcatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcgt ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatt
//
LOCUS       DAELSY010000302         1421 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.304, whole genome shotgun sequence.
ACCESSION   DAELSY010000302 DAELSY010000000
VERSION     DAELSY010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.304"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..737
                     /locus_tag="KPG09_003836"
     CDS             <1..737
                     /locus_tag="KPG09_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBG0968367.1"
                     /translation="IDVDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIFIAF
                     NYNKLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSRIKI
                     DTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLLVII
                     YLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINFGNP
                     LGKILYIAIALLLIFSLFSLIKSLLL"
     gene            857..1333
                     /locus_tag="KPG09_003837"
     CDS             857..1333
                     /locus_tag="KPG09_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG0968368.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLRVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
BASE COUNT          517 a          173 c          191 g          540 t
ORIGIN      
        1 atatagatgt agattcaaaa ttaatagtag atatggattt tataaataag aaaaataaaa
       61 taataaaaaa atttaaaata atctatttaa taccgatact atttactttg gggatgagta
      121 tttttatagc atttaattat aataaattgc cagactcaat tgccactcat tggaacataa
      181 atgggtctcc tgatgttttt atagataaat catttcttaa tgtatttaaa ttaatcggta
      241 ctgacttttg tttaatggtt ttattatata taacttccat tggatccata aagtcaagga
      301 ttaagataga tacaaataga attgaagaaa gtagagttga aaacattaaa tatttaaata
      361 aaattggtta tttgttttta atattgatga ttataatgac aacacagttt tttactactc
      421 ttttatcaat aaaaacaaaa ctatcaactg ctatgaatat tacaacgctt ttagttataa
      481 tttatttaat agcaacatat attaattccc caaatttaaa attcaactca tcttattctc
      541 cagaagaaga tgagaagtat tggatagcag gcatcatgta taataatcca aatgacccat
      601 cattaatggt taataaaagg tttggtattg gatggactat aaattttggc aatcctcttg
      661 gaaaaatact atatattgct atagctttac ttttaatttt ttcattgttt agcctaataa
      721 aatctctctt actttaaaat accataaaag gtatcttact tgtattattg ggtattatat
      781 tttgtaggat aagaaatatt tcatattatc gttgaagtat agtatttata tattactaag
      841 aatggagatt ttcataatgt tgtcaaaagc tgaaatcaaa tattttaatg aatgtgcttc
      901 tgagatactt tcttctgaaa aagttcaact tatgagaaca tttcctcatc atggcaatgt
      961 ttcatgtctt gaacatagtt tgtcagtagc atattatagt tatctattat gtaaaaaatt
     1021 gcacttacgt gttgatattc aaagtgttat tagaggagct ttactacacg acttttttct
     1081 ttatgattgg cattacaaag gtgatagaaa aggattacat ggatttactc accctagaga
     1141 agcattaaaa aatgcaactt tattttttca aataaatgaa aaagaaacgg acataatttt
     1201 aaaacacatg tggccactta ctgttaagcc acctagatac aaagaagcat ttattgtatg
     1261 tttgttggat aaattttgct gtttagttga gactttaaag atacattctt tattatctcc
     1321 ttaccatgta taggacaatt cccacaacac ttttataatt taattttgaa gaggtattaa
     1381 gtattatatt caacttgata cctctttact atttattatt t
//
LOCUS       DAELSY010000303         1398 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.305, whole genome shotgun sequence.
ACCESSION   DAELSY010000303 DAELSY010000000
VERSION     DAELSY010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.305"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1299)
                     /locus_tag="KPG09_003838"
     CDS             complement(<1..1299)
                     /locus_tag="KPG09_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0968369.1"
                     /translation="MDTYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTE"
BASE COUNT          518 a          180 c          184 g          516 t
ORIGIN      
        1 ctcagtataa gagatatcca attttccatt gtacttatta acaatttctc taatattctc
       61 aataccatat ccgtgagaat tactatcttt tttatctgaa ataaatagtt cttgttttct
      121 attgatagga tttaccttgt tgtttgtcaa taaatagcta aaatactcct tctcacaacg
      181 tgcctttaat accacttttc tatttgacaa atcaggaatt ttcaaagatg cttctatagc
      241 gttatctaaa gaatttgaaa aaatagtaca taaatctata ggttctatac ccaagtgatt
      301 gtctatatct atattaaagt gcacatcaac accattttct tccaaacttg tatgacgaag
      361 atttaaaatt gagttaagag cttggttttt gcaaaattgt ttgccactaa gagtacctat
      421 acctattttt aactgattaa aatacttttt agctccagca atatcttcaa tttctaaata
      481 cgattcaatc atttgaaagt gattattcat atcatgtcgc attttgcgta tttgaatttg
      541 ttgattttct agtaagttat agtatttttc ttttaggtga tttgcttgtt tttctatctt
      601 catctttaca ttttcaatca atggctgtaa aagaagtatc acaccgatat ttgccagaat
      661 tgctgacaag atgattagat aagtgccata aggtaagttg cctaagataa tattcttatt
      721 actacccata gggattaaat tagtaggtgg cagaataata gcaattaata ttgataaaaa
      781 tgaagatata cataccatat caataaaaat ccatatttta tttgataagt attgttttat
      841 attgaccaat ctattcttta ggaaaatatg tattaaaaac caaaataata atttaattag
      901 agcagaaatt atctggacta ttgaaagtat taaaatatat tcttttgagt gtataagttc
      961 ctcatatata gggttgttta taaaaagaaa attaagccca attataatag gataaagtat
     1021 catgacaact gatattatag gaattaattt gcttttgcta catattataa gtagtataaa
     1081 atatccgaat agtgtaaaca aaatatttac ggggtcggaa agccatatta tcatttgact
     1141 aataatagag tagcccaaaa tcaatatgat tttgaaaatc caatgattcc taaagtctat
     1201 aaaataggaa gtcaacttat aaagaaaaaa ggctatgaaa atattgatta atattagaat
     1261 aaaatttatg cttgtagaga ttaaagaata agtgtccatt taatatacct ttctattaat
     1321 taagattctg gaaactaagt tttaaaaaat taaatcttaa aaattaaaat tctggaaatt
     1381 agatttcaaa gaattgat
//
LOCUS       DAELSY010000304         1394 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.306, whole genome shotgun sequence.
ACCESSION   DAELSY010000304 DAELSY010000000
VERSION     DAELSY010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.306"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..493)
                     /locus_tag="KPG09_003839"
     CDS             complement(<1..493)
                     /locus_tag="KPG09_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0968370.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVKTLAVFLI
                     IFIV"
     gene            complement(480..1247)
                     /locus_tag="KPG09_003840"
     CDS             complement(480..1247)
                     /locus_tag="KPG09_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0968371.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALKGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTMDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
BASE COUNT          481 a          239 c          124 g          550 t
ORIGIN      
        1 taactataaa tattattaaa aataccgcta gtgtcttaac taaggccttt atacttaagt
       61 tcatatttac tactatcatt tcattcatta attttacaag catcatagtc ataagctttg
      121 aaaataacag tcctaaaaat atacctatta gtattgcaac tatacccatc aaaaaagttt
      181 catataaaaa tatttttcct atctgatttt tcctcattcc aagtaaacta taagttccta
      241 cttctcgcct acgtctattg aaaaaaaatg agtttgaata atatataaat aaaaatacaa
      301 ataccactat tacaattgaa cttgtattaa tactcatcct agttttttct ccaagtgaac
      361 tcaacgcttc attatactct atagatttaa aactaaaaaa tacaaataca ctaaatacaa
      421 ttgaaataaa gtacattaaa taattaaaaa aactattctt tacattttgc attgcaattc
      481 taaataaagt catttttatt aactcctcca attaaggaaa ctgtatttac tattttatta
      541 tagaactctc tgttactttc tccctttaca atttctgtga atattattcc atcctttata
      601 aagagtattc tattgcaaaa actcgcactt atagcatcat gagttaccat caatattgtt
      661 ttattgctgt ctttgtttaa ttttaataga caattcaaca aatccctagc agatttagaa
      721 tctaatgctc ctgtaggctc atctgcaagt actaatgatg gttttgttgc tatagctcta
      781 catacagctc ctctttgctt ttgtccacca gaaacttcgc caggatattt atctaatata
      841 gattctatat ttaattcttt tgaaatctct tttattctat tttctattac tgatactctt
      901 tcatttttta aagaaagagg caacattata ttttctttta ttgtaagtgt atccaaaaga
      961 ttagaatctt gaaatacaaa gcctagatta tcacgtctaa aatctgccaa ttcattttgc
     1021 ttcattttaa tagtattctt tccatttata taaatatctc ctgatgaagg taaatccatt
     1081 gtagaaataa tatttaaaag tgttgattta ccagcaccgg aagaccccat aataccaaca
     1141 aattctcctt tataaacctt gaaagttatt ccttttagag cttcatgttt tgaacctttt
     1201 cttccatact ccttttttat attttcaaca gataatattt cttccatagt taataatcac
     1261 ctttcatcac aattagttat ttatcaattc tcacaaatat attatatata ttaaaatggc
     1321 ttttaaacat ttaattatat taatttaagg ttacattttc gtaagtatta attatatttc
     1381 ttcaaattta ttgc
//
LOCUS       DAELSY010000305         1336 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.308, whole genome shotgun sequence.
ACCESSION   DAELSY010000305 DAELSY010000000
VERSION     DAELSY010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.308"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            27..101
                     /locus_tag="KPG09_003841"
     tRNA            27..101
                     /locus_tag="KPG09_003841"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="KPG09_003842"
     tRNA            116..200
                     /locus_tag="KPG09_003842"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            209..292
                     /locus_tag="KPG09_003843"
     tRNA            209..292
                     /locus_tag="KPG09_003843"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:243..245,aa:Leu,seq:tag)
     gene            322..398
                     /locus_tag="KPG09_003844"
     tRNA            322..398
                     /locus_tag="KPG09_003844"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Arg,seq:tct)
     gene            406..481
                     /locus_tag="KPG09_003845"
     tRNA            406..481
                     /locus_tag="KPG09_003845"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:439..441,aa:Gln,seq:ttg)
     gene            570..658
                     /locus_tag="KPG09_003846"
     tRNA            570..658
                     /locus_tag="KPG09_003846"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:604..606,aa:Ser,seq:tga)
     gene            662..737
                     /locus_tag="KPG09_003847"
     tRNA            662..737
                     /locus_tag="KPG09_003847"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:695..697,aa:Phe,seq:gaa)
     gene            744..817
                     /locus_tag="KPG09_003848"
     tRNA            744..817
                     /locus_tag="KPG09_003848"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:778..780,aa:Met,seq:cat)
     gene            832..908
                     /locus_tag="KPG09_003849"
     tRNA            832..908
                     /locus_tag="KPG09_003849"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:866..868,aa:Ile,seq:cat)
     gene            932..1008
                     /locus_tag="KPG09_003850"
     tRNA            932..1008
                     /locus_tag="KPG09_003850"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:966..968,aa:Pro,seq:tgg)
     gene            1016..1092
                     /locus_tag="KPG09_003851"
     tRNA            1016..1092
                     /locus_tag="KPG09_003851"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1050..1052,aa:His,seq:gtg)
     gene            1101..1176
                     /locus_tag="KPG09_003852"
     tRNA            1101..1176
                     /locus_tag="KPG09_003852"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1134..1136,aa:Lys,seq:ttt)
     gene            1184..1257
                     /locus_tag="KPG09_003853"
     tRNA            1184..1257
                     /locus_tag="KPG09_003853"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1216..1218,aa:Cys,seq:gca)
     gene            1264..1335
                     /locus_tag="KPG09_003854"
     tRNA            1264..1335
                     /locus_tag="KPG09_003854"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1296..1298,aa:Asn,seq:gtt)
BASE COUNT          320 a          310 c          355 g          351 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaatgcgc ggatgtggcg gaattggcag acgcactaga
      241 cttaggatct agcgccattg gcgtgggggt tcgactccct tcatccgcac catttttgtt
      301 tgatatacat atataaattg tgggtgcata gctcagctgg atagagcaac gcccttctaa
      361 ggcgtgtgtc cggggttcga atccctgtgc gctcaccaca tatattgggg attagccaag
      421 tcggtaaggc acatgacttt gactcatgta tgcgtaggtt cgagtcctgc atccccagcc
      481 aaatgatgta ttactaagct atatttgaac tatttttatt ttaaatatag ttaggttcaa
      541 ttttagaata agtttaagta tccgaatgtg gagaggtgtc cgagtggttt aaggagctgg
      601 tcttgaaaac cagtgacttc gaaaggggcc gtgggttcga atcccaccct ctccgccaaa
      661 tgcccagata gctcagtcgg tagagcaggg gactgaaaat ccccgtgtcg gtggttcgat
      721 tccgcctctg ggcaccaaaa tatggcggta tagcttagtt ggctagagcg ttcggttcat
      781 acccgaaagg tcataggttc gactcctatt accgctacta aaatttaatg tgggccatta
      841 gctcagttgg ttagagcgcc cggctcataa ccggtaggtc tggggttcga gtccctgatg
      901 gcccaccaca ttaaattaat taatataaat tcgaggtgta gcgcagtttg gtagcgcaca
      961 tggtttggga ccatggggcc gggggttcga gtcccttcac ctcgaccaat ataaggtggg
     1021 tatagctcag ttggttagag cgccagattg tggctctgga ggtcgtgagt tcgactctca
     1081 ttatccaccc caattcatat ggtctattag ctcagtcggt agagcacctg acttttaatc
     1141 agggtgtccc gcgttcgagt cgcggataga tcaccaaaaa ggtggcgaca tagccaagtg
     1201 gtaaggcagt ggactgcaac tccttgatcc ccagttcgaa tctgggtgtc gcctccaaaa
     1261 tattccaagg tagctcaacg gtggagcaac cggctgttaa ccggtaggct gtgggttcga
     1321 gccccaccct tggagc
//
LOCUS       DAELSY010000306         1331 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.309, whole genome shotgun sequence.
ACCESSION   DAELSY010000306 DAELSY010000000
VERSION     DAELSY010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.309"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            83..748
                     /locus_tag="KPG09_003855"
     CDS             83..748
                     /locus_tag="KPG09_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968372.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
BASE COUNT          534 a          153 c          149 g          495 t
ORIGIN      
        1 atccaattta tgatattatt taaatataat ataagtttta taaagtatat taaatatatt
       61 ataagatgga ggtcgagtaa ttttgaaagg aaacatagta caatataatt ttgcagatat
      121 agaggaagaa gtatattcgt tggattatgc tatagcatgg aatacagatg aggaaaacgt
      181 aaacataata ccatttacaa ataaattctg taaagaatct atagaatctt tttgcctagg
      241 taaaattaat aactttgttg aaatattaaa tgaaggtttt gttgaaaatc atcattatgt
      301 acatttagat aaaatgatta gtgttcctaa gaaaaaggtt aatttagtat atcaacaaga
      361 cacacatggt tatcttctaa gagatgataa tgacaacctt attcctgcaa aaataacttc
      421 agagcaatct aaatctatat caagtaagat ggagttattc tgtgctggtg aagaaaaatg
      481 tttaattaat atattactaa aggctgaccc atcatatatt ttagatgtag atagtataaa
      541 agacaaaaac attctaaatc taggttatga gtctattgac agatataaag aatacaattt
      601 tgatgatgat aagattttaa tattttttat aaataaaaaa cgttattcag tgattatgaa
      661 aaaaacaaac aactctgata acgatttagt ttccagaaac aatgctatta aagaattatt
      721 tactaataaa gcaggaaatc ttaactaata ttataatttt aaattatatt attaattaaa
      781 ttaataatag tatacaaaga ggctgtctca aaatagagat aagcttcttt ttcattgttg
      841 aaataataaa atatatttaa ttctatataa aagagagtgc ctcataaact aaaaagttca
      901 ttttgagaca ccctcctatt tataataaac aaataaatcc ttaaacctat cgagtgatta
      961 taatttttag attgggttat cttgcatttt taaatttaag gtaactttct actctaaact
     1021 ttaatctcta atcaatatca tcaactgtaa caacttacaa ttaaatcttt aataatcaaa
     1081 ctttagacta tctatattaa aactcaaatt agatttagga tttatttgga tatcacattt
     1141 attttattaa ctagtaatca attattttta cttataaact tattcttccc taattctatt
     1201 attaaatact tttaattatt tttcaatacc atattttttt aatttttact attaaatcac
     1261 aaaatattat cttactccat cacaactact tttgatatca tttgagaatg aatcttcatt
     1321 ttaatagtac t
//
LOCUS       DAELSY010000307         1323 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.310, whole genome shotgun sequence.
ACCESSION   DAELSY010000307 DAELSY010000000
VERSION     DAELSY010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.310"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            17..685
                     /locus_tag="KPG09_003856"
     CDS             17..685
                     /locus_tag="KPG09_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968373.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWENDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            844..>1323
                     /locus_tag="KPG09_003857"
     CDS             844..>1323
                     /locus_tag="KPG09_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBG0968374.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     "
BASE COUNT          606 a           96 c          211 g          410 t
ORIGIN      
        1 taaagggtga ttttttatgg aaaatataac tagttatttt acaacaatac tatgtgtatt
       61 tatatgctta tcatctgtat ttatatttac tcaactagct agagtattta ttaataagaa
      121 gaagattaat caaaaaatta aatcaagaaa cggatttagg tatgatagag attttattga
      181 agccagaaga gaagaaatac atataaagga taacaataat aataaaaata agagtaacaa
      241 taagaaatta aaggaagaaa aagtatttaa atacgataat ggtgatttat ataaaggtga
      301 gtttgttgat ggaaagaaaa atggatttgg aatatatatt ttttcaagta aggaaaagta
      361 tgaaggatta tggaaagatg acaagatgca tggaatagga aaatatactt atagagatgg
      421 ttcaatatat acaggtgaat ttaaatacgg attaaaaaat ggtttgggaa aattaacata
      481 tcccaataat gacatttata agggatattt tttagataat aagaaaaatg gtaagggtgt
      541 attatataaa aatgatggaa ataaacaagc aggaatatgg gaaaatgatg aacaatgtaa
      601 aagtttagat tttaaagatt taaataataa taaaataaat aatgtttatc aaaatagatt
      661 aaataacaat aaattaaaag tttaattatt ctaataaaaa aatctttatt tatgatggaa
      721 tataggatat atctatgttg taatttgtaa aatcttccta tatcatataa aattaagtat
      781 tgattagtct attacataaa tatagaatat aaatatatcc atttaataat aaggagaaga
      841 gatatgaaag aatatgtagt tgtggattta gaaactacag gattagatcc atataaaggt
      901 tgtgaaataa tagaaatagg aataacagaa ataaaaaatg aacaaattgt aaaaaactat
      961 tcaaggttga taaaacctaa ggggataatt tcttcattta taacagaatt gacacatata
     1021 agtaatgaaa tggtagaaaa tgaagaacca ctagaattag ttttacctag atttagaaaa
     1081 tacataggtg atagaactat aattgcacac aatgcgaaat ttgatttaaa atttttaaat
     1141 tactatctta ggatgttaga tttagaacca ataaacaatt atatatgtac agtagaatta
     1201 ttaaaaaaat gtaaaagtta taaaggaaaa aacaagaaac ttgaaacagc ttgtgcttat
     1261 tacaacatag agaatataaa tgctcataga gctgacagtg atacattagc aactgcaaaa
     1321 cta
//
LOCUS       DAELSY010000308         1317 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.311, whole genome shotgun sequence.
ACCESSION   DAELSY010000308 DAELSY010000000
VERSION     DAELSY010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.311"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            108..224
                     /gene="rrf"
                     /locus_tag="KPG09_003858"
     rRNA            108..224
                     /gene="rrf"
                     /locus_tag="KPG09_003858"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            232..306
                     /locus_tag="KPG09_003859"
     tRNA            232..306
                     /locus_tag="KPG09_003859"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:264..266,aa:Asn,seq:gtt)
     gene            312..397
                     /locus_tag="KPG09_003860"
     tRNA            312..397
                     /locus_tag="KPG09_003860"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:346..348,aa:Leu,seq:taa)
     gene            413..488
                     /locus_tag="KPG09_003861"
     tRNA            413..488
                     /locus_tag="KPG09_003861"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:446..448,aa:Met,seq:cat)
     gene            496..570
                     /locus_tag="KPG09_003862"
     tRNA            496..570
                     /locus_tag="KPG09_003862"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:529..531,aa:Glu,seq:ttc)
     gene            585..658
                     /locus_tag="KPG09_003863"
     tRNA            585..658
                     /locus_tag="KPG09_003863"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:617..619,aa:Gly,seq:tcc)
     gene            664..739
                     /locus_tag="KPG09_003864"
     tRNA            664..739
                     /locus_tag="KPG09_003864"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:697..699,aa:Val,seq:tac)
     gene            745..821
                     /locus_tag="KPG09_003865"
     tRNA            745..821
                     /locus_tag="KPG09_003865"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:779..781,aa:Asp,seq:gtc)
     gene            832..906
                     /locus_tag="KPG09_003866"
     tRNA            832..906
                     /locus_tag="KPG09_003866"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:864..866,aa:Gly,seq:gcc)
     gene            916..989
                     /locus_tag="KPG09_003867"
     tRNA            916..989
                     /locus_tag="KPG09_003867"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:950..952,aa:Arg,seq:tct)
BASE COUNT          381 a          265 c          304 g          367 t
ORIGIN      
        1 attaagatat atgtgtagtt tttagagtgc taactctaaa gaaactagta ttgctaggtt
       61 ctataaactt attaaaaact taatagaaat attaagcata aagattatgt ggttacaata
      121 gcagagagga tacacctgtt cccattccga acacagaagt taagctctct agcgctgatg
      181 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgttaaatg ttccaaggta
      241 gctcaacggt ggagcaaccg gctgttaacc ggtaggctgt gggttcgagc cccacccttg
      301 gagccaaaaa agccgaagtg gcggaactgg cagacgcaca ggacttaaaa tcctgcggga
      361 cttacctctc gtaccggttc gattccggtc ttcggcactt ataaaacaat atcgcggggt
      421 ggagcagttg gcagctcgtc gggctcataa cccgaaggtc gtaggttcaa gtcctgcctc
      481 cgcaaccatt aaaacggctc gttggtcaag aggttaagac accgcccttt cacggcggta
      541 acaggggttc aattccccta cgagtcacca attcaattaa atatgcgggt gtagctcaat
      601 ggtagagttc tggccttcca agccagctgt gagggttcga tccccttcac ccgctccaaa
      661 aatgggacca tagctcagct gggagagcac ctgccttaca agcagggggt cacaggttcg
      721 agccctgttg gtcccaccat tcgcggcctg gtagttcagt tggttagaat gccagcctgt
      781 cacgctggag gtcgagggtt cgagcccctt ccaggtcgcc aaattaaata agcgggaata
      841 gttcagtggt agagcgcaac cttgccaagg ttgaagtcgc gagttcgaat ctcgtttccc
      901 gctccataat aacttgggtg catagctcag ctggatagag caacgccctt ctaaggcgtg
      961 tgtccggggt tcgaatccct gtgcgctcat taagaaaagt ttactataaa ttatagtaaa
     1021 cttttatatt gtttaaaaag acacaggaaa catgtatatt acctgtgtct ttttatttat
     1081 aaactttaaa ctatgtaaaa attacagtat attctaatag agttacagta tattcattaa
     1141 tattgtaaac atacggaggg taatgtataa tagtctacaa gatgtttatg aaggcatcag
     1201 ttaaatataa ctaattagat aagagttata tacattatat tgtatacttt atggtattta
     1261 tatattgaaa taacttatga aattatttat aaagagattt atgagcatat ttttgtc
//
LOCUS       DAELSY010000309         1310 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.312, whole genome shotgun sequence.
ACCESSION   DAELSY010000309 DAELSY010000000
VERSION     DAELSY010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.312"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            612..1082
                     /locus_tag="KPG09_003868"
     CDS             612..1082
                     /locus_tag="KPG09_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG0968375.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIEFVKQCENSKE"
BASE COUNT          622 a           79 c          218 g          391 t
ORIGIN      
        1 atataaattg atagtggata ttctatttaa ttaaatttaa aaagctacct ttacttataa
       61 gtataattaa ggtagctttt taaatagagg tagtgtgatt aaagagaatt tttaaatata
      121 aattgtgaag ctttaatata gatgacaata tatattttgc aaatatgaca cagtgttttc
      181 gtatttaaga tgataaaata aataggaatt aatgaagata tttaaaaaat aaaaataaag
      241 aattaataga agatattaaa gagatagaga taaaagtgtt aatagaagat attgaaaaag
      301 taaaaataaa ggattaatag aagatactga agaggtagaa acaaaagtgt taatagaaga
      361 tattgaaaaa ataaaaacaa aagattaata aaaggtactg aagagataga gataaaagtg
      421 ttaatagaag atattgaaaa ataaaaataa aagattgata taatatactg aagaggtaga
      481 aacaaaagtg ttaatagaag atactaaaaa agtaaaaata aaggactaat aaaaggtact
      541 gaagagatag agaaaaaaat gttaatagaa gatactgaaa aaatagaaat aaaaaattag
      601 gaggaaaatt tatgaaatat caggtttcat tattagcagt gaaggatatt gaagtatcaa
      661 aaaaatttta caaagaatta tttgagcaag aaattgaatt ggacttaggc aaaaatgtaa
      721 catttaaagg aggatttgct atacaagaag acttcgcatg gcttacaggg attaatccag
      781 atacaattat acaaaaatca aataatatgg aactttattt tgaggttgat gattttgacg
      841 aatttattga aaaactcaat tattataaag atgtagagtt tgtccataag ccactaaaac
      901 atgaatggca acaaagagta gtgcgtattt atgacccaga taagcatatt attgagatag
      961 gagagtctat ggaagttata gctagaagat atttatctga gggatactct gtagaagaaa
     1021 cttctaaaat aattcaacat ccaattgaat ttgtaaagca gtgtgaaaat agtaaggaat
     1081 gattgtttaa attataatta tttagtagaa attaattgta gtggaactaa ttcaagataa
     1141 tatataggac tagtatttga caaatgtcat aaattaaatt aaaataaaga aaatattaat
     1201 aaagaaaaat aaggggaata ttaataaaga aaaaataaat ataatattaa aatatattaa
     1261 taaatttgaa tatttataat atttttaatt tgaagtagga atacttaaac
//
LOCUS       DAELSY010000310         1252 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.313, whole genome shotgun sequence.
ACCESSION   DAELSY010000310 DAELSY010000000
VERSION     DAELSY010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.313"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            91..588
                     /locus_tag="KPG09_003869"
     CDS             91..588
                     /locus_tag="KPG09_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0968376.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            638..1162
                     /locus_tag="KPG09_003870"
     CDS             638..1162
                     /locus_tag="KPG09_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG0968377.1"
                     /translation="MKSVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKDLQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKVPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILNYIRKL"
BASE COUNT          517 a          112 c          224 g          399 t
ORIGIN      
        1 atagtaacta attactataa tataaagtaa ctagttacta tttaaaatta tcaaaaaatt
       61 aaaatctaag ataaagagaa gtgtataata atggatgcag aaagtttaat gaaaaaatat
      121 atggtagatg atgatagtag aaaacaagct atatttgcaa gtatatttgt aatgcaaaat
      181 aaacttcaaa ctacatgtga taaattagac ccagaattaa caatgaaaca atggttactt
      241 ttagctattg cggactctgt agatgaaact cttacattaa ccaaacttgg agaattaatg
      301 gggtgctcaa gacaaaatgt aaaaaaacta gctattgctt tagaaaagaa aggttttata
      361 caaattgagc aaaatgaaaa agattcaaga gctatctgtc ttgtgacaaa agatagagtg
      421 caagagtatt ctaaaaaagt aagaggtatg caggaaaaag tattacagct tttatttgaa
      481 gattttacag agaaagaagt ggaacagtta ttttttggaa ttatgaaatt aagtatagga
      541 attgaaaatg tgaaaaatta tgtagaaaat aatgacgttg gagaataagc aagtatcaaa
      601 tcaactttgt atgacatcag agaggggggt aatttatatg aaaagtgtag tagtgattta
      661 taagagtaag tatggttcaa caaaaaaata tgctaaatgg ctttcagaat tattgtcttg
      721 tgatattttt gaaactaaga atattaatat agaaaaggtt atgaagtatg atacgattat
      781 tttaggagga ggtctttatg cttgtaatat agcgggaatt tctttcttaa aaaaaaatta
      841 taaagatttg cagaaaaaaa atttagtagt atttggcgta ggagctgctc catatgatga
      901 tgaagtaata aagaaattaa gagaagtaaa ctttaaggat gaattaaaca aagttccttg
      961 cttttattgt cgtggggcat gggatgagac aaaaatgtct tttatagatt atactctttg
     1021 tagttttttt aaaaaatcta tagctaaaaa agaacgtcaa ggtcttaaac cagcagaagc
     1081 acctttattg tctggagaag cctttgattg gtgtaataaa gaaaatttaa ctccaatact
     1141 taattatata agaaaacttt aagaggtata tatttataat tatttattat ctaaagttag
     1201 atttcataga aacttactag acattattta taaaataata ttattaaaga tg
//
LOCUS       DAELSY010000311         1244 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.314, whole genome shotgun sequence.
ACCESSION   DAELSY010000311 DAELSY010000000
VERSION     DAELSY010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.314"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..235)
                     /locus_tag="KPG09_003871"
     CDS             complement(<1..235)
                     /locus_tag="KPG09_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968378.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLAL"
     gene            442..>1244
                     /locus_tag="KPG09_003872"
     CDS             442..>1244
                     /locus_tag="KPG09_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0968379.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIIGNSYEESILDFFCESNEDNYMTEIS"
BASE COUNT          535 a          116 c          174 g          419 t
ORIGIN      
        1 ctaatgctaa tgatgttatt gcagtatcta atcttcttat taataaactc atttctatat
       61 attcaagttc tattaaatat tgataaaata tttctagttt aacagctttt tctctatcta
      121 tatttccata ctttaactct aatttatcta aagatttttt attacaataa ttaactttat
      181 gtttaattaa atatcttatt gtagcaccta ttacaatttt cactctaaca agcataaata
      241 taatattcca tccaaaagtt aagaggggat atctttttta tgagaggaat ggataaaata
      301 tttataactg acatctaact gatatttaag ctttaaaatc aaaaccatat tgattttcta
      361 aaataataac tacttgattt agtaaataat aaagaatata gtaactatat agacaacaaa
      421 aagaaaagga gtgtataacc cttggaaaaa cagtatttta caacaggtga atttgctaaa
      481 ttgtgtggta tatctaagca gacattaatt ttctatgaca aaataggtat tttttctcct
      541 gaatataaag ataaaaataa ttatagatat tactcagttt atcaatatga tacacttgat
      601 atattacaat cattaagaga aataggaatg agtttagagg aaataaaaga gtatatacaa
      661 aatagaactc cccagctttg tgtgaaaatg cttaaagaag aagaaaaaaa gataagagaa
      721 aaaataaaaa aactaagaaa gataagctct aagatgcaaa atagaataaa tataacagtt
      781 gaaggaattt ctaaaatgaa tagtgaagaa atttatatat ccaaaaagtt tgaagaatat
      841 ttagttctat caagcgattt gagtaatatg agtaatagtg attttatgat ggaattaata
      901 gatttcacca attattgtaa atcgaaaaat ttgtatcaag gatatgagtt gggagtaata
      961 gtaagtaatg aaaatataaa aaatggggac tatttaagta tatcaagttt ttatttgaag
     1021 atagataaaa aaataaagga taaaaagcta tatgtaaagc cagaaggaat gtatgcttgc
     1081 ataaaacata ttggaaaata tgaggattca tataaatctt acgaaaaatt gaaaaaatat
     1141 atatatgaaa atgaatataa aattattgga aattcgtatg aggagagtat acttgacttt
     1201 ttttgtgaaa gtaatgaaga taattatatg actgaaattt caat
//
LOCUS       DAELSY010000312         1238 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.315, whole genome shotgun sequence.
ACCESSION   DAELSY010000312 DAELSY010000000
VERSION     DAELSY010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.315"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(1..295)
                     /locus_tag="KPG09_003873"
                     /pseudo
     CDS             complement(1..295)
                     /locus_tag="KPG09_003873"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            355..848
                     /locus_tag="KPG09_003874"
                     /pseudo
     CDS             355..848
                     /locus_tag="KPG09_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT          489 a          181 c          140 g          428 t
ORIGIN      
        1 tctctattat tttaactaat tgttcatatg tatcaaattt ccttaaatag tacatttcca
       61 cctttaaaat tccccaaatt ccttccatag gagcattgtc aatacaacga ccagctcttg
      121 acatactttg tatcatacct tgtgctaaaa gtttcgattc aaacacctta ctagtatact
      181 gaaaacctct atcactatga aaaacaggtt ttgcatttgg atgtagtttt attactttat
      241 caaaagtttc gaataccaag ttattattat tcgattttcc aataacatat gaaattaata
      301 gatataacgc cttctatttt acagtcttat ataaataaca tatacaatga gcacatgtta
      361 aattcaataa aaaaccaagt cgattttata aaatcagtat tgaaagaatc ttatagatta
      421 aaagaaatat cagaaaatat ttgtgatttt gttacaaccc caatcaaaaa aaatagttca
      481 acttctaagt tttatacaaa gcaagaaact caattattat tagaaaaatc aaaaaatatt
      541 cctataggta taccaatttt tttgatacta actcttggat tacgttttgg tgaagctgta
      601 tcacttatat ggtcagatgt agaccttgat aaaaaatctc acatgtaaat caaacattag
      661 tttatgtata caataaaata atcttcaaag accctaaaat ccctagatcc aaaagaaaat
      721 tatttgctcc tgatgaactt ataagtttgc ttaaagggca aaaattaaaa caaaataagt
      781 taaaattaca gggtatattg aaaaatgaat ttgatttgat atgcttaaat aaaagattca
      841 acccgtgagt tcaaccaact tttgttgttc attttaaaaa actattaaga gataataatc
      901 ctagagatat aaaagtacat gaattaatac atgcaaatgc gactttgata ttactttcag
      961 gtactaacat aaaagctatt tataaaatat tagtacatgc agatataaaa atagctatga
     1021 ataaatattc gatattctca cttattagat gaaatggata aagaaacatc tgaaaatata
     1081 agcaaaatac tattcaaata atgttcagtt ttatgtcaat taaatgtcac tcaaagtgct
     1141 ttttttccat ttgtcagtta aatgtcactt aaagtacctt tttcctattt gtcagttaaa
     1201 tgtcagtaat atataaataa gatatattta taagaccc
//
LOCUS       DAELSY010000313         1235 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.316, whole genome shotgun sequence.
ACCESSION   DAELSY010000313 DAELSY010000000
VERSION     DAELSY010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.316"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1121
                     /locus_tag="KPG09_003875"
     CDS             <1..1121
                     /locus_tag="KPG09_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG0968380.1"
                     /translation="EEYKNKYSTGDKIILSLKSFDNKELYYHETDNILNSKDEIDRLT
                     SSKIFKIIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYMIYNNHRA
                     LEYGLDNLDKLYSHKFIYNLHHILGENCLDSEEYEYRTEKVYVCDSKGEIIHTGLDPL
                     KIYNFMDELIIFMENSKVNDLVKSTIIHFYFVYVHPFSDGNGRTSRALSYLYLINKGY
                     DTFKEFSISYIISKNRTKYYKAILDVENKGYNLTVFIEFMLKSIIQSINEMRDMHDRK
                     SLESILKEELFENDITLSATEEHILKYICSKDNYSMTLENYIKKNKGRYLKAGIKEIE
                     LVDQLMEVFNNLEEIEILSREKDVYKVNEKYLNMLEIS"
BASE COUNT          533 a          107 c          189 g          406 t
ORIGIN      
        1 tagaagaata caaaaataaa tactctactg gagataaaat tatactttcc ttaaagagtt
       61 ttgataataa agaactatac tatcatgaaa ctgataacat attaaattca aaagatgaaa
      121 tagatagatt aacatcaagc aagatattta aaataataga agataaaaat gatttagcct
      181 tagagtctta ttattctagt gtaatagaag gtgccttttc aactagaaag attgcaaaat
      241 ctataattag aggaaagatg aaaccaagta ataaaagtga gtatatgatt tacaataatc
      301 atagagcctt agaatatgga ttagataatt tagataaatt atatagtcac aaatttatat
      361 ataatttaca tcatatattg ggtgaaaact gtttagattc agaagaatat gaatatagaa
      421 ctgaaaaagt atatgtatgt gattcaaaag gtgagattat acatacagga cttgatcctt
      481 tgaaaatata taattttatg gatgaattaa taatttttat ggaaaatagt aaagtaaatg
      541 atttagtaaa gagtacaata attcatttct actttgtata tgttcatcct tttagtgatg
      601 gtaatggaag aacatcaaga gccttatcat atctatattt aattaataaa ggatatgata
      661 cttttaaaga gttttcaata tcatatataa tatctaaaaa cagaactaag tattataagg
      721 ctatactaga tgtagagaat aaaggatata atttaactgt atttatagag ttcatgctaa
      781 agtctataat acaatctatc aatgaaatgc gtgatatgca tgataggaag tctttagaga
      841 gtatattaaa agaggaatta tttgaaaatg atataactct atcagcaaca gaagaacata
      901 tattaaaata tatctgtagt aaagataatt attctatgac attagagaac tatatcaaaa
      961 agaataaagg tagatactta aaggctggaa taaaagaaat cgaattagtt gaccagttga
     1021 tggaagtgtt taataatctt gaagaaatag agattttatc tagagaaaag gatgtatata
     1081 aagttaatga gaagtacttg aatatgcttg aaataagtta aattgaagca tataaaaata
     1141 aaaagtatat aaagttatat aatataataa taagccctct atgaatcgct cttaattgag
     1201 ttttcttaga gggttaaatt tttcattctt aaaat
//
LOCUS       DAELSY010000314         1213 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.317, whole genome shotgun sequence.
ACCESSION   DAELSY010000314 DAELSY010000000
VERSION     DAELSY010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.317"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..253
                     /locus_tag="KPG09_003876"
     CDS             <1..253
                     /locus_tag="KPG09_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968381.1"
                     /translation="TKQITVEVNEILLQEISKITNDENNILTDLINESIVNYILENKK
                     HNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(682..1059)
                     /locus_tag="KPG09_003877"
     CDS             complement(682..1059)
                     /locus_tag="KPG09_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0968382.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT          497 a          170 c           97 g          449 t
ORIGIN      
        1 tacaaaacaa attacagtag aagttaatga aattttatta caagaaatat ctaagataac
       61 taatgacgaa aataatattt tgacagattt gataaatgaa tcaattgtaa attatattct
      121 tgaaaacaaa aaacataaca ttgaaaataa agatttagaa gaagtgatta aattttacaa
      181 aaaagaaaat actgaaaaac ctaaaagtgc agcaagagct atagctgaag caaaaactca
      241 taccctgtat taatcaaata aaaaagttcc taaaaactag gaactttttt atattaataa
      301 ttattaatat atctcaatat ttatctcaaa tttatcaata taagcatgtt taatacaatc
      361 tattgtatac ttatcttacc ctagaaataa tcaaaacaaa aattatgccc tttatatcta
      421 acaattaatt tacatgatta atatacaaaa attctgactt ttaaattcgt atacaatatc
      481 aaactaaaat aatactaatg acaaacccaa tataaattaa attcaaacag gtttgatttt
      541 acatcaaact tattgtatta atattaaata acaagacaaa atatttataa aaattacaaa
      601 ttttacataa aactttatat tttattcatt taactaatta ttgtaaaaac tatttatttt
      661 atgataatta actacaccaa attattcatc ttttttaata tctttaaccc atttttcaac
      721 agagttcaat tcttcttcat ttaccccctt tttgaacaag gatttcataa aactctcaat
      781 agaacttcca tgcatattat ctaaaaaagt ttttgtttca aaatgtaaat actcttcttc
      841 tccaataaga actgtataat gtgtatgtct atcaatcttt cttgcactta agaattgctt
      901 atttactaat cttgataaaa gtgttagtgt tgtagtttgc ttccaattat atagtttttc
      961 catagcttca atgacatctt ttgatgctac tgtaaaatct gaactccaaa taaatttcat
     1021 gacttttaat tctgctggtg gtatcttagt aatagtcata tgattttaca ctcctctcaa
     1081 acttctacat aattaaatac tatattaata gacttttcga gtcaaaggtt ttatttaaaa
     1141 tatctcatcc aaatttatta atacaaaaaa tttatatttt tattcaaaat taaacacaca
     1201 atctattaat ata
//
LOCUS       DAELSY010000315         1198 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.318, whole genome shotgun sequence.
ACCESSION   DAELSY010000315 DAELSY010000000
VERSION     DAELSY010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.318"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            20..487
                     /locus_tag="KPG09_003878"
     CDS             20..487
                     /locus_tag="KPG09_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG0968383.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFILENVKRFKVMGCCLLINTIIECVIG
                     YNSNTSLIMIFGDNSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
     gene            498..713
                     /locus_tag="KPG09_003879"
     CDS             498..713
                     /locus_tag="KPG09_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0968384.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
BASE COUNT          431 a          141 c          156 g          470 t
ORIGIN      
        1 ataaaggaga tgatatttta tgattacaaa attttttagt aatatattat cagtatttct
       61 atattttcta ttatcacttt taggagttgc tggtataata ttcttatgta tgatacctat
      121 gtttttatat gattttagtt ctgacaaagt aatcgcttta attgcaagaa tactatattt
      181 ttctgactac tttatagtca tcttattctt gatatttata attaatagta ctaagtattc
      241 cccattcata cttgaaaatg taaaaaggtt taaagtaatg ggatgttgtt tattgataaa
      301 cactataatt gaatgtgtaa ttggttataa ctcaaatact agtcttataa tgatatttgg
      361 agataatagt ggcggtatct caccacttat ggttatttct tttatatttg cactaatgtg
      421 ctttgtaatt gcagaaacat ttgataaagc tataaaaata aaagaagata atgatttaac
      481 gatatagagg tgatgttatg gcaatagtag ttaacctaga tgtaatgatg gcaaaaagga
      541 aaatatcact taaagatttg gcagaaaaaa tagatattac aaatgcaaat ttatcaattt
      601 taaaaactgg aaaagcaaaa gctattagat ttaccactct aaacgaaatt tgcaaagctt
      661 tagactgtca gccaggtgat atacttgaat atgtagaaga tgaaaatgat taaaaaagag
      721 tgtttagaaa cactcttttt tattatacta tatgattttt attcttttac tatattattg
      781 acattaatat taactttgtt taaagtcata acaaaatacg ttctaagaaa gaaactgttc
      841 ctttttctat ttgttatcca ttacctataa gaactgtatc acactaaatc atacttttaa
      901 attgataatt ttgccacaga ttcctatata cattcttttc tacctaatat ttatttaaac
      961 tgttagcaaa acatggaact ttttcattta cttatacctc catttatatt attgcatagt
     1021 ttatagctac gtgttataaa ttacattatt gatgatttat ctaacatttg taatctttta
     1081 gatactcttt gaagttacaa cactgttata ctctaattaa gtaaactaaa tctgatacaa
     1141 gttggtgatt atatgaaaag aaaactttca acctatttta aaggatatat tttaggtt
//
LOCUS       DAELSY010000316         1189 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.319, whole genome shotgun sequence.
ACCESSION   DAELSY010000316 DAELSY010000000
VERSION     DAELSY010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.319"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(329..529)
                     /locus_tag="KPG09_003880"
     CDS             complement(329..529)
                     /locus_tag="KPG09_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG0968385.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(754..930)
                     /locus_tag="KPG09_003881"
     CDS             complement(754..930)
                     /locus_tag="KPG09_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968386.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(984..1073)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          443 a          167 c          116 g          463 t
ORIGIN      
        1 atgttttaat tgctttttat taacaaatta ctaaaagtgt tatatcagaa ttgaatacaa
       61 aagaaagagt atgtatttat gagatagttg aaaattttga tgtgccacaa ctaaaattat
      121 tatattacta tatattctaa ttaaaagagt acttgtaggt ttaagtacat actttataaa
      181 tatatttttg tcagatttat tttataaaaa gtatttagac taagtattat ttttaagtat
      241 tttcttgttg aagttatttt ttcctttttt acaaaatgcg taaataaaaa aaagactctt
      301 aaaagagtct ttccgaaaaa tctatatatt ataatatagt tatattttct gcttgaggac
      361 ctctagcgcc tttaactata tcaaagctta ctttttgacc ttcttctaat gatttaaatc
      421 ctgaagtttg tatagctgag aaatgagcaa atacgtcatc tccactttcc attgatataa
      481 atccaaatcc tttttcatta ttaaaccatt ttactattcc attattcatt aaaaatactc
      541 ctattaacct atatcaagat atacttgatt tacttgtgta ttaccacaaa tactattata
      601 tcatagatta acataaatgt ctatttaatt ataattttta gttaacattt ttatttattc
      661 ttaatatgaa aataaattta aacattttat attaccaata tattaatgta aatgtagata
      721 aaactaaaaa gggttttcta gaagaaaatc tgtctaatta tacttactta ccaataccat
      781 cacttgatgc aagcaagatt ccacaacttt ttctccgtac tctaatataa aatcatctaa
      841 ctgtgtatct ataaaatcac aataatcata tgaactacca atataagcag ttagcatatg
      901 tgtacattta tccattttat ctctaaacat aatgtattta tccccctaaa aaactttcat
      961 gaaaaacaaa aagaaaatat ctttttggca actggctgac ataacttatt ttataagcgt
     1021 tagtccctat agctttgcgt cactaaattt ctctagtttt gccgatttag ttttattcta
     1081 cagattaata atacaataga attagctatt attcaacata tctagctgaa aaattaataa
     1141 atttatatat gaacgttaaa ttatttgaaa aaataaaaag cccctctgt
//
LOCUS       DAELSY010000317         1147 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.320, whole genome shotgun sequence.
ACCESSION   DAELSY010000317 DAELSY010000000
VERSION     DAELSY010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.320"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            428..895
                     /locus_tag="KPG09_003882"
     CDS             428..895
                     /locus_tag="KPG09_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0968387.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDILGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKGIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEIGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          450 a           76 c          178 g          443 t
ORIGIN      
        1 attagaaaga ttatgaaaac taaattagaa gaactaaaaa ctatattttt attaattata
       61 atatttgaat aatcttttta atcatataaa atgcatatac ttgtaatatt taaaggtgat
      121 attaatatta ttagtgtata ttaatattcc cttgaaatta aaaaaacata gatatttcag
      181 taatctaaaa tagtctacaa aaataacagt tttagcaaaa ttaataagaa tacatataga
      241 tttaaaccta aaagtatgaa catgtctgtt ttttacaaga ttaaaattta tattatatga
      301 taaaattaac ttaactattt caataagact tataattttt ttctttatac tagaccttat
      361 tgaaaccttt atagtttaat tctaattata tattataaat aaagtatgtg tatattaagg
      421 aggtaatatg aagtttttaa atgttttaat agtagttgaa gatattgaga aatcaaaaaa
      481 gttttattat gatattttgg ggttaaaggt aatctgtgat tttggagaaa atgttgtatt
      541 agaaggtaat atatcattac aagaaaaaaa gctttggtta gaatttatta ataagagtga
      601 cagtgaagtt aagttcaatg gaaatgatgc tgagttatat tttgaggaag ataattttga
      661 tacttttgtt gaaagattga gtacaatgaa aggtatagat tatgttcatc tagctataga
      721 gcataggtgg ggtcaaagag caattagatt ttatgattta gatgggcata ttattgagat
      781 tggagaaact atgtcatctg tgtgtagacg ttttttagat agtggtcttt caatagatga
      841 ggtagctaaa agaatggatg taactgtcga atatattgaa tcagttttag aatagatgga
      901 ttatattaaa gtaaaactat attttgtata gaagtaaggg ctgtaagtaa acttgtatgg
      961 aaacaaattt acttacagtt tttgtttttg taagatacag aatataatat ttgttataat
     1021 tttatttgga tttttaaaaa gtgcatgatt ttccttacat attataatag aatttaatat
     1081 ttattggatg atttaaattt agaaaaatat aagtattagc ttataaaaga taattatact
     1141 tactatc
//
LOCUS       DAELSY010000318         1146 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.321, whole genome shotgun sequence.
ACCESSION   DAELSY010000318 DAELSY010000000
VERSION     DAELSY010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.321"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>1146
                     /locus_tag="KPG09_003883"
     rRNA            <1..>1146
                     /locus_tag="KPG09_003883"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          293 a          253 c          352 g          248 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtactag gtgtcggggg ttaccccctt
      601 cggtgccgca gctaacgcat taagtactcc gcctgggaag tacgctcgca agagtgaaac
      661 tcaaaggaat tgacggggac ccgcacaagt agcggagcat gtggtttaat tcgaagcaac
      721 gcgaagaacc ttacctaagc ttgacatccc aatgacatct ccttaatcgg agagttccct
      781 tcggggacat tggtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg
      841 gttaagtccc gcaacgagcg caacccttgt ctttagttgc catcattaag ttgggcactc
      901 tagagagact gccagggata acctggagga aggtggggat gacgtcaaat catcatgccc
      961 cttatgctta gggctacaca cgtgctacaa tgggtagtac agagggttgc caagccgtaa
     1021 ggtggagcta atcccttaaa gctactctca gttcggattg taggctgaaa ctcgcctaca
     1081 tgaagctgga gttactagta atcgcagatc agaatgctgc ggtgaatgcg ttcccgggtc
     1141 ttgtac
//
LOCUS       DAELSY010000319         1134 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.322, whole genome shotgun sequence.
ACCESSION   DAELSY010000319 DAELSY010000000
VERSION     DAELSY010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.322"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            152..862
                     /gene="rgaR"
                     /locus_tag="KPG09_003884"
     CDS             152..862
                     /gene="rgaR"
                     /locus_tag="KPG09_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBG0968388.1"
                     /translation="MFRVVICDDEKIQRSILKEFTKKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT          454 a           93 c          183 g          404 t
ORIGIN      
        1 aatttaagtt taaatattaa agagaatttc tataagtagt ttaatcgaca acttaaattt
       61 ataaagaaat ttaaatttga aagctagtga gtacagtttg gaaattgaaa taaagtaaac
      121 taaatgatat tttagatttg gaggtgatat tatgttcagg gtagttatat gtgatgacga
      181 aaaaatacaa agaagcatac taaaagagtt tacaaaaaaa cttttgtctg aaagatgtct
      241 aaattatcaa atactggaat tttcctgtgg agaagattta atatccaaat acccagaaaa
      301 aattgatatt atatttttag atatccaaat gaaagatatc aatggaattg aggtggctaa
      361 aaaaataaga aagtttgatg aaaaagtaga aattatattt acaacagctt tttcagagta
      421 tgccccccaa ggttatgagg ttcgtgctta tagatattta gtaaagccta tagagtatag
      481 taactttgca atgggtgtaa atctttgtat tgataacctt caaagaaaga aggagcgtta
      541 tatagtttta aatagtaaaa aaggatttaa tagaatactg atagattcta ttttatatgt
      601 tgaaactgta aagagagatt tagttgtaca tacaataggt agagattata aagctaatat
      661 gagcatgaaa caagcagaaa aattactgtg tgaaaatggc ttttttagat gtcatacaag
      721 ttttattgtt aatttaagaa gaatagaaga tcttagggat aatatgatta ctattaatga
      781 acaatttata cctataagta agtatagact taaagattta aaagtagcat taacaagctt
      841 gttatgtgat ataatgtatt aattttaaaa aggtaattga aaatatcgta aaaaagattt
      901 aatgtcatca cagggctatt gattaaaact gtcttaatat ataaaatgtt atttatatta
      961 agacagtttt attatgcaaa atatcagttt atagactttt aatattgttt aatatgtata
     1021 tgtagagttt ccattacagt agcatgatta ttaattagta gcctgattat taatatacta
     1081 ttattgattt ttatatgcat tatatgtatt tttagaatat gattaccttt ttaa
//
LOCUS       DAELSY010000320         1126 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.323, whole genome shotgun sequence.
ACCESSION   DAELSY010000320 DAELSY010000000
VERSION     DAELSY010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.323"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            257..769
                     /locus_tag="KPG09_003885"
     CDS             257..769
                     /locus_tag="KPG09_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968389.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEDYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKAKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
BASE COUNT          513 a           80 c          200 g          333 t
ORIGIN      
        1 attggagtaa ttaaaaattt ttattttgca aaaataagta tttatttata tggataaatt
       61 tgattttaaa tctatcaata tagataaaat cttattattt aacttcaact attccttaaa
      121 aaagcaactt ttaaggaata gttgaaacaa aaagtttggt tacataatag taattttagt
      181 tacaagaatg aaactgtaaa acttaggatt ttctttatat aatttcattg agaacattta
      241 atttaggagg taaggtatga aagtaaaaaa atttaaaagt ggtatagtaa tggcattaac
      301 agtaatggca attggagcat cattgacaaa tgtatcagct atggaattaa acaagagtaa
      361 agagtttgaa actaaataca atgaaatgaa gagggatttt caagaggatt atggaaaaga
      421 agacaaattt aatataacat tggatgatga atttacaaaa gaagaagtaa atggagctaa
      481 agggattata aagacaacta ataaaatgaa taatgaggtt gtagtattaa attattttga
      541 tgaaatgaaa tctgggattg aatttgagtt gaaggcaaaa gcagaaaaaa atgcaatttt
      601 gagagatgaa tttcaacaag gttatggaaa ggaagataaa ttcaaagttg tgttagatga
      661 tgaatttaca aaagaagagg taaatggagc taagggcatt ataaagacta cagataaaga
      721 gacaggtgag gttatcgtat ataattactt tgatgagctt gaaaaataat gcttaaagat
      781 tactttgatt gaatgaaaag gtaattgtgt tgagaatatt aaatgactga ataaaaacag
      841 ttgtatcaaa aacaaacaat aatagaatgt aagaatgatt gtgtcgaaaa tattaaatga
      901 ctgaataaaa aatagttgcg tcaaaaacaa acaataatag aatgtaagaa tgattgtatc
      961 aagaatataa aatgattcaa caaaaaacga ttgtgttaaa aacaaacaac aatagaatgt
     1021 aagaatgatt gtatcaagaa tataaaatga ctgaataaaa acgattgcgt caaaaataaa
     1081 caataataga atgtaagaat gattgtatca agaatataaa atgatt
//
LOCUS       DAELSY010000321         1054 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.324, whole genome shotgun sequence.
ACCESSION   DAELSY010000321 DAELSY010000000
VERSION     DAELSY010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.324"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            117..869
                     /gene="cwlD"
                     /locus_tag="KPG09_003886"
     CDS             117..869
                     /gene="cwlD"
                     /locus_tag="KPG09_003886"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBG0968390.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT          471 a          108 c          161 g          314 t
ORIGIN      
        1 ataaacatag tgaattaaat aggggcagaa atataatatc attacataat aatatattaa
       61 taccaataaa taaagatata tcaatattaa aaaaatttaa atgtttgtac taatatatgg
      121 aatattatag tgtttttata agtatacata tacagggtga tgttgtgaga aagtacataa
      181 aacatataat ttttagtttt gcaatgatat gtctagtagt agtatccata tttgaaatta
      241 aaaatatttc tgaagatgtt atcaagtata tgccagtaac aaacaaaact ataattttag
      301 atgctggtca tggaggcatt gatccaggtg cattaaataa ggataagagt acatctgaaa
      361 aggatattaa cctagcaata acacttaagc ttagagagct tatagaatca agtggtggcc
      421 ttgtaatatt aactcgagaa gatgacagta gcctttataa agaagaaaat aataaaacta
      481 caagacaaaa atataatgag aatttgaaaa atagaaaaga aataatatct aattctaatg
      541 ccaatatgtt tgtctctata catcttaatg catttgaaca atcaaaatac tatggagctc
      601 aaacttttta tcctaaagac aaacaagaca gtaaagaatt atcgaagtgt attcaagaag
      661 aacttaaaag agtagtagat aagacaaaca acagagaagt caaaccaagg gatgatatat
      721 atcttttgaa agaaaataat attccatcag tactgataga atgtggtttt ttatcaaatg
      781 aaaaagagtg taaactctta actgatgaaa catatcaaga aaaaatagca tgggcaatct
      841 acataggaat acaaaaatat ttaagttgaa ataagtataa aaataggtct aaatatgtaa
      901 atagcttgta ttaaagcagt taaaatgcat taatataggc tatttttatt ttgacaaaaa
      961 aatatttaaa ataaaagtta aaaagttgtt gacttagaat aatatagatg atattatata
     1021 tgagtgccca aaaggagcac caaaataaga caaa
//
LOCUS       DAELSY010000322         1008 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.325, whole genome shotgun sequence.
ACCESSION   DAELSY010000322 DAELSY010000000
VERSION     DAELSY010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.325"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(282..>1008)
                     /locus_tag="KPG09_003887"
     CDS             complement(282..>1008)
                     /locus_tag="KPG09_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG0968391.1"
                     /translation="LDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIA
                     SDEGFPFHDIPLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKAMGLEIVKPFCK
                     YDDKIEVGVICKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYP
                     FVAREVAELGINLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGM
                     VDYFKNLGLESEFLQGKYYLEDL"
BASE COUNT          384 a          155 c           98 g          371 t
ORIGIN      
        1 tttttttaaa taaatattta caataatcta aaataaatat ttataaattc taatcaatcc
       61 taaataataa tctaaaatac atcaaaaaat aaactaaaaa atatcgaaaa ataatttaaa
      121 agacatcaaa actaaatctt ctaatcaaca attaaaagtc tattttctta gtttaaaaaa
      181 taaagataga ttttttcaaa acactggata aataggtaaa aaaataaaca gttagtaaaa
      241 taaattttta ctaactgttt atttttattg tttaatatgt cctataaatc ctctaagtaa
      301 tattttcctt gcaaaaactc tgattctaaa cctagatttt taaaataatc aaccatgccc
      361 atacacgcaa atttttctgt agaataatga gtagcaccta taacattaat taaattatct
      421 tttgctattt gatggaactc taatactggt tcaaaatgtt ttaaaggctt agtaaaccca
      481 gtaatatata gatttatacc aagttcagca acttctcttg ctacaaatgg ataacttcct
      541 ccccctgctg caatagcaac taaaccatct tttaatacag aatctccata atcatacaat
      601 ttcacatcat gaccaacaga ttttttaatt aattccttaa tctcttctat tgattttagt
      661 gatgttttac atataactcc tacctcaatt ttatcgtcat atttacaaaa tggtttgact
      721 atttctaaac ccatagcttt tgcaaaacta acagaagttg aatagtcact atagttgtct
      781 agtggagaat gtaatacata aaaagaaatt ctacgatttt tcatctcttc catataagat
      841 agtggtatgt cgtgaaatgg aaaaccttca tctgatgcta tgtatcccat tgcgtgatgt
      901 gaaaaaatta aaatatcttt ttcatcttta tctataactt gcttaattat ttctttatca
      961 ggaaaagtag ttgtgtatac tttattaata ttttgagcat tatctaat
//
LOCUS       DAELSY010000323          993 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.326, whole genome shotgun sequence.
ACCESSION   DAELSY010000323 DAELSY010000000
VERSION     DAELSY010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.326"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            159..665
                     /locus_tag="KPG09_003888"
     CDS             159..665
                     /locus_tag="KPG09_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBG0968392.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          397 a           93 c          155 g          348 t
ORIGIN      
        1 aaatatgaaa actacttatt gcgaaagtaa ttgatttgtt gtattataat ttacatgaat
       61 tatgtaggac aatcaaaagt cagtattata agagtacata tattcaaatt tttaacaatt
      121 aatttatata attaaaaaat gaagggtagg atgaagtcat gggttttaga gaagtaaaaa
      181 ttgaggaact acagtttaat ccatttacta agattggcaa agaatggtta cttataacag
      241 ctggtgattc agaaaagttt aacacaatga ctgctagctg gggtggtgtt ggagtctatt
      301 ggggtaaaaa tgttgttaca acttacataa gaccacagag atatacaaaa gaatttgtag
      361 atagtaatga tacatttaca gttgccttct ttgatgaaac gtatagagag gctttaaata
      421 tttgtggtac tatttctggt agagatataa ataaaataga aaaagcagga cttacaccat
      481 attttgtgga tgatacggta gcttttgaag aagctaatat gattatagta tgtaaaaagc
      541 tatatcatgg taatatgcca ccagaaaact ttgatgctaa agaaaatgat aaaaaatggt
      601 atccagagaa agactatcat actatgtaca tttcagaaat tataaaagtt cttgtaaaag
      661 aataattata ataaaaaata tatgaaaaga acttattata agtaaatgac tgtgctttct
      721 tatctgatat ctaagtagat gctaccaact aaataaatat taattttaaa taaatactat
      781 tataaaaaag atgaactata tttactatag ttcatctttt tatttatata agcacttata
      841 taagtagaga tttatttgaa taaaagtatt tatttgaaat atttgagcat actttataag
      901 tatttttatg ctaactagaa acacttaatt tatggcattt gtatttgatt tattttaatg
      961 taaataattt caaagttaat tttattcaat ata
//
LOCUS       DAELSY010000324          963 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.327, whole genome shotgun sequence.
ACCESSION   DAELSY010000324 DAELSY010000000
VERSION     DAELSY010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.327"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            283..942
                     /locus_tag="KPG09_003889"
     CDS             283..942
                     /locus_tag="KPG09_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG0968393.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSNISIFVLIHIILKIKIRKI
                     R"
BASE COUNT          355 a           74 c          111 g          423 t
ORIGIN      
        1 tatttaacca tatcaaaata tattttacta tttttaatta ttacaatata aacctgtctt
       61 ttgttattat aggtaaacta aaagtaagaa atgattttta tatttttaaa ctttattatt
      121 ggtaacttat ctgtaaatat tttaaaatgt actatagaaa gttgttaaaa atattaaata
      181 attatattaa atttaagtgt gttttttatg ctataattag tatgtatttt ttatgatata
      241 ttttataaaa aatataattt aatcattttt gaggatatta ttatgttaga tgcatttata
      301 acaattgttt cttctattat agaaatatgg tcatgtaaaa aagtttttga ttatactagt
      361 cagtcaaaga ccgacttaaa aaaactaaat atgtattttc tcttttctgt ttttcttatt
      421 atttcttctt tatatataaa tataggagct aatagtagaa tcttgatatg tatatttgta
      481 atgttcgttt tctataagtc taactataag gttaatatat ataaatgtct ggtaatctca
      541 tttttttatt ggttaatatt aataggtata aatggattaa gtatgttatt agtagcaaca
      601 gtaagcaatt tggataatat gaatgtttta cttagtggca gtttatatag atatgaagca
      661 atattcttgg gaaagataat gttaggtata ttactttatg tttataaggc ttcttttaaa
      721 aattttaaaa taagtaaaaa agagatatac atatgtattc ctatgatagc aaatttattg
      781 tcattttttg tactttatga ttatattatt aatcttgtaa aaatctctcc tgaaagaaga
      841 actgcttttt tagtaatagt tttactatta atattatcta acatttcaat ctttgtatta
      901 atacatataa tacttaagat taaaattagg aaaataagat aaattttaat acgattcatt
      961 taa
//
LOCUS       DAELSY010000325          960 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.328, whole genome shotgun sequence.
ACCESSION   DAELSY010000325 DAELSY010000000
VERSION     DAELSY010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.328"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            138..893
                     /locus_tag="KPG09_003890"
     CDS             138..893
                     /locus_tag="KPG09_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HBG0968394.1"
                     /translation="MAHICFEDICYLLLKEAMKLKLIPHNKIVLFEDDLSIGNIFDVN
                     NYELRENIFKIRNVFENNPKLLFKPDLLEDRKLSKCRNIIIWCANNPKDLCGVYYVVS
                     KFQDKNIKIALVDEKKTEDALIRYSFTSEMSPEDFPFFLDKTLEIGFNEKLEFKNKWD
                     VLVKENSIFRALVGNEIKSVEETYFDKYILRNISDKWQPLIEVVANCMFEDEMRVKDW
                     FILWRLEKMASLKIVKIKGSKGDFYSDKEIRKV"
BASE COUNT          413 a           59 c          162 g          326 t
ORIGIN      
        1 tataaatatt aaaaatttta gtattaataa aaaagatatt acattaatat gaaaatatag
       61 gagattagaa gtgtttgata attacagttt atatattata ataaaataaa ttgttaattt
      121 taaaagaggt gaattttatg gcacatatat gctttgagga tatttgttat ttgttattaa
      181 aagaagcaat gaagttaaag ttaataccac ataataaaat tgtattattt gaagatgatt
      241 tatcaatagg taatattttt gatgtaaata actacgaatt aagagaaaat atttttaaga
      301 ttagaaatgt ttttgaaaat aatccgaaac tattattcaa gccagatttg ttagaagata
      361 ggaaattatc aaaatgtaga aatataataa tatggtgtgc aaataaccca aaagatttgt
      421 gtggagtata ttatgtagtg agtaagtttc aagataaaaa tataaagatt gcattagtag
      481 atgaaaaaaa gacagaagat gctctaatta gatattcatt taccagtgaa atgtctccag
      541 aagattttcc attcttttta gataagacac tagaaattgg ctttaatgaa aaactagaat
      601 ttaaaaataa atgggatgtg ttagttaaag aaaattctat ttttagagca ttagtaggta
      661 acgaaattaa aagtgttgaa gaaacttatt ttgataaata tatattaaga aatatcagtg
      721 acaaatggca acctttaatt gaagtggttg caaactgtat gtttgaagat gaaatgcgtg
      781 taaaagattg gtttatactt tggagattag agaagatggc aagtttaaaa atagtaaaaa
      841 taaaaggaag taaaggggat ttttattctg ataaggagat aagaaaagtt taaaaattga
      901 atttataata aattgtatta aataatgggt ataactatga agaaaaatat cttaacagta
//
LOCUS       DAELSY010000326          944 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.329, whole genome shotgun sequence.
ACCESSION   DAELSY010000326 DAELSY010000000
VERSION     DAELSY010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.329"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            162..821
                     /gene="cprR"
                     /locus_tag="KPG09_003891"
     CDS             162..821
                     /gene="cprR"
                     /locus_tag="KPG09_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBG0968395.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
BASE COUNT          385 a           59 c          176 g          324 t
ORIGIN      
        1 tttgaaatat aaagtttttg tatctaaaat attttatttg taaaatagta attaattatt
       61 gtaagctata atatgagagt attatggtta atgtgaattt taaagatatt attaaagata
      121 taaattagaa tactattaaa cggataaatg gaggagttat tatgtctaag atattagtag
      181 tagatgatga gttagatatg ttaaagttga ttgaaaatgt gttgaaaaga gatggacatg
      241 aagtaaaggt aattagtgat gtagaaaagg ctatgaacat ggattttaat tattttaatc
      301 taataatatt agatgttatg atgcctaaga tggatggttt tgaagtatgt aaaactataa
      361 gaaataaggt tgattgtcca atactgtttt tgacagcaaa aaatatggaa agtgatatta
      421 tgtacggtct tggcataggt gcagatgact atataaccaa accttttggt gtaggagaat
      481 taagggctag gataactgct catttaaggc gagaaagtag agaaaaaaag aatttattaa
      541 ctatctcaaa tgttcaattt aactttttag gcaaagaagt ttcaattgat ggtaaaaaaa
      601 ttaattttac taaaagtgaa tatctaatat gtgaatttct agcaaaaaat aagggacaag
      661 tattttcaaa agaaagaata tatgagtcgg tatatggttt tgatggagaa agtgatataa
      721 gtactatcac agagcatata aaaaatatac gaagcaaatt aaaacaatat gatatttata
      781 caatagaaac tgtatggggg atagggtata aatgggtgta ataagtataa gtgttgctta
      841 gtgaaaatat tatatatatc tacaaaatgt ttgtaaaata ttaatctata tattaaaatt
      901 taatttatag attaatattt tttgtaaatg acaaaattgt aagg
//
LOCUS       DAELSY010000327          898 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.330, whole genome shotgun sequence.
ACCESSION   DAELSY010000327 DAELSY010000000
VERSION     DAELSY010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.330"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            109..>898
                     /locus_tag="KPG09_003892"
     CDS             109..>898
                     /locus_tag="KPG09_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968396.1"
                     /translation="MSNIITNLIESVFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQS"
BASE COUNT          357 a           89 c          130 g          322 t
ORIGIN      
        1 tgtaccattt ttcataaata taatataaat attttgttta tttacgtatt ttcttagtaa
       61 cttgtacgta taaattataa taaataaatt ataaggtagg ggataaattt gagtaacata
      121 ataacgaatt taatagagtc agtatttact aatccaataa ctatattgtt tttacttgca
      181 ataactgtgt attctgtttt taaaataata gaaaataaca gagtatattc atttgtaagt
      241 tcaaaattag acgaaatcaa tagagattat aagacaagtg agttttataa aaaggtaagg
      301 gatgactact atacatatag taaagaaaat ccttatgcag acgtaaatat aacttcattt
      361 atagaggaag ttgtatctga tttaaagcat aataatttgc cattgttaga aaaaattaga
      421 agtataaaaa attcatcttc aataagtata ttacttgggg ttttaggtac gtttgttggt
      481 ctttctacaa tgcttttatg tgttgatact aaagacatta taaattcact tccttcaact
      541 attagtagta tgcaaactgc atttacaact agtatatttg gagttgtatt ttctctaatt
      601 ataggttact ttactaagat aaaagattgt gaacatgttt tgatacaaat aatgttaaaa
      661 tctgaaaatt tactaacatc tgaaataact cattttaagt cagaaagaat ggatttaaaa
      721 gtagaagagg taaaaaatac aataaaacaa ataagtaagt ctatagaagc tattgaaaaa
      781 tttgataaaa tatctaaaga tttaaatgac tttaatgatg aatttatcag tggtatagaa
      841 gctttaaaaa gccttttaga aggttctcaa agttctatta agacatttga ccagagcg
//
LOCUS       DAELSY010000328          881 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.331, whole genome shotgun sequence.
ACCESSION   DAELSY010000328 DAELSY010000000
VERSION     DAELSY010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.331"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(106..819)
                     /locus_tag="KPG09_003893"
     CDS             complement(106..819)
                     /locus_tag="KPG09_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0968397.1"
                     /translation="MINIIICEGKKSERVILNDYINTFFYNYNFEYNIKEFENGGDLL
                     KKYPNNVDIIFMDIDLNDENGFNVSKEIKKFDESVRIIFVSSSNKYIKKGYEIGVYRY
                     LEKPLKYSLFKEAISSYLKECYKNEAILSIKCKCKIYFIKVDDILYLEVLKNDLTIHT
                     EKGIYTFKNSMKEIESNLEEHNFFRCHKSYLINLMKVNMLDIENNIVFINQNSIIVSR
                     YKIKLLREKLNSCLVDVII"
BASE COUNT          312 a          116 c           61 g          392 t
ORIGIN      
        1 gatgaaaggt atatttttaa ctataattag tttatattat aaattttttc atattttaca
       61 atagatatat tctattagaa tagaatataa gggcaaaaat ttacctcaaa ttattacatc
      121 taccaaacaa ctatttaact tttctctcaa taattttatc ttataacgac ttacaattat
      181 tgaattttga tttataaata caatattatt ttctatatct agcatattta ccttcattaa
      241 atttataagg tagcttttat gacatctaaa aaaattatgt tcttctagat ttgattctat
      301 ttctttcata ctattcttaa aagtatatat tcctttttca gtatgtatag ttaaatcatt
      361 ttttaaaact tctaaatata aaatatcatc tacctttatg aaatatatct tgcatttaca
      421 ttttatagat aaaatagctt catttttgta gcattctttt aaatatgatg atattgcttc
      481 tttaaataga gagtatttta agggcttctc taaatatcta taaactccta tctcataacc
      541 tttctttatg tatttatttg atgaggatac aaatataatt cttacacttt catcaaattt
      601 tttgatttct tttgaaacat taaatccatt ttcatcattt aagtctatat ccataaatat
      661 gatatcaaca ttattaggat acttttttaa taaatcgcct ccattttcaa actctttgat
      721 attgtattca aaattgtaat tataaaaaaa ggtatttata taatcattta gaattaccct
      781 ctctgatttt ttaccttcgc aaataataat attaatcata ttacacccct tatataattt
      841 tttattttaa attttataaa attctatcta acatttatta t
//
LOCUS       DAELSY010000329          879 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.332, whole genome shotgun sequence.
ACCESSION   DAELSY010000329 DAELSY010000000
VERSION     DAELSY010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.332"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(323..802)
                     /locus_tag="KPG09_003894"
     CDS             complement(323..802)
                     /locus_tag="KPG09_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG0968398.1"
                     /translation="MPNKLIKNLLSGILQILFFLLGLVIVVGGFKSFMYLCFSGEATL
                     QGTISGILMFILGVSYFIIIKSLIEVLSSSEHSLFVKDNVKRFRIIGYLLLLNSIMEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          352 a          125 c           61 g          341 t
ORIGIN      
        1 attatgcctt tatgctatta aaacatctat tttatactga ttatttacaa atatatctaa
       61 attaattata aattttattg aataagactt ttataattat acctttaata aattataaaa
      121 aagcactttt ataaaaaaat gtgcttttaa tatctactaa taaagttctt tattttaatt
      181 aaaacataat tatattttag tattataatc ttttaatttt agcattatag tttatgtatt
      241 ataccctaat atattttatt ttaaattact agttaatcac tcttttctgg ttatttatct
      301 cctatatatt aaagtgtcac acctaaatag ttaaatcatt atcctctttt atctttattg
      361 cctttacaaa tccatctgca attacaaaac tcataagaga agttataaaa tatacaaata
      421 ctggaacagt aaaataaaat ccaaatccta aatctaggaa tctcattcct tttccagtag
      481 taccaaaagt tgaaataaac tccattatag agtttaaaag caaaagatat ccaataatcc
      541 taaatctttt tacattatct tttacaaata agctatgttc agaactactt aatacttcta
      601 tcaatgattt tataatgata aaatatgata cacctaaaat aaacattaat attccagaaa
      661 tagtgccttg tagagttgct tctccactaa aacagaggta cataaaactt ttaaatcctc
      721 ccacaacaat tactaaccct aaaagaaaaa ataaaatttg taatatacca cttagtaaat
      781 tttttataag tttattaggc atcataccaa ctcctttaaa atattttata tatagtataa
      841 tacattatat tatatcgttt ttcaataaaa atattataa
//
LOCUS       DAELSY010000330          876 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.333, whole genome shotgun sequence.
ACCESSION   DAELSY010000330 DAELSY010000000
VERSION     DAELSY010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.333"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(95..388)
                     /locus_tag="KPG09_003895"
     CDS             complement(95..388)
                     /locus_tag="KPG09_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBG0968399.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(394..609)
                     /locus_tag="KPG09_003896"
     CDS             complement(394..609)
                     /locus_tag="KPG09_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968400.1"
                     /translation="MRTLSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
BASE COUNT          281 a          114 c          100 g          381 t
ORIGIN      
        1 ttatttagaa gaaggggaaa tcttaaaaaa atttgaatat ccccttttta ttatttaatt
       61 aaaatgaaat agttagaaat atattttatt tagtttagtc aataacattt tgggattctt
      121 tataactaca tgttaactgt tgaataggct tatcatatgg tttaacccat cctttattaa
      181 tacttaattc tgtaagtgct gtgtgacctt ctaccatttg agttagtgaa gctgaatata
      241 tagctcttaa ttcaggtgta ctacttgtta atgcagaatt aagatacata ttggcagatg
      301 ctttagcaga tgaaagcata cttaacatta ttactttatc gtctatataa gtattgttct
      361 taattagatt ttctatcata tttctcatgt tatctatacc tcctcagaaa ttagtatttc
      421 attttcaact ataaattgtt gtatagcttt aattcttcct tctgttgcta atatactaga
      481 ttctgattgt ctttttaggt cttcatctga tattaacata tttattgctc tttgcataag
      541 taatccgtca gactccattt ttataactgc tgctaaagat attttttcag tttctgaaag
      601 tgttctcatt taatcatctc ctatattgta atttatgctt taatagtatg tctttctttt
      661 caaggaaata accattacac ttttttacag tattttatat tctctttata tcaaagttta
      721 gtcaacttaa tatattaata taattttgta ggatatttat cattaaatat ctataaaata
      781 aattatttga atattcataa atcaaaattt atttgtatgt acttcttcaa gagtcaatat
      841 gaattttttc aaaaaattaa ttttgatgat aatcat
//
LOCUS       DAELSY010000331          838 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.335, whole genome shotgun sequence.
ACCESSION   DAELSY010000331 DAELSY010000000
VERSION     DAELSY010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.335"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(90..800)
                     /locus_tag="KPG09_003897"
     CDS             complement(90..800)
                     /locus_tag="KPG09_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0968401.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELEKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVHIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLEP"
BASE COUNT          271 a          118 c          103 g          346 t
ORIGIN      
        1 aaattaaatt tgaaaaaaca aaactctaaa aactgagctt taaaaaatta agtttcaaaa
       61 ataaaattct agagattgaa cttcaaaaat taaggttcta acatatacct tgtaaatttt
      121 cctacaacct cttgatattt tttacggctt ataggaattt tttcattctg aactttggca
      181 taagtttgag ttatactatc tacatggttt agattgacaa tatacctttg atgcgtacgt
      241 ataaaattag agggaagaac agtttccata tcatttaatt ttccgtagta ttctaatgtt
      301 ttatcaaagg taactacagt taattttctt agctcgcttt taaagtagag gatatctttt
      361 gtattaattt tacaaagatt agttccaata tgaactgtaa taaagttttc tttccattgt
      421 tctaattttt ttagtgcttc ttttaatact ttacatattt tttcagtttt ataaggtttt
      481 aagatgtagt tcaaagctcc tacatcatac ccatgaaata catagtcaga aaaacctgtg
      541 acaaagatga ttatagtatc ttttgaatgt tttcgtatag tttttgctat gtcaacgcca
      601 ttactttttt caagttcaat atctaaaaaa ataatatcta tgtaataatt ttcgtgttct
      661 agattgtata ctagactttc tccatttgaa tattctttta tttggtaggg gtagccccac
      721 aaatccattt ctcttgtaat gattttattc agcatgtttc tttgttgttt ttcatcatca
      781 caaatacaaa tcgaagtcaa ttttttatct cctttcaagt tttatattta tatagtta
//
LOCUS       DAELSY010000332          806 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.336, whole genome shotgun sequence.
ACCESSION   DAELSY010000332 DAELSY010000000
VERSION     DAELSY010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.336"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(67..750)
                     /locus_tag="KPG09_003898"
     CDS             complement(67..750)
                     /locus_tag="KPG09_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBG0968402.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
BASE COUNT          320 a          135 c           72 g          279 t
ORIGIN      
        1 tacaatattt gctatatata aagtatatca aataacagct tcattttttt gtaaatttac
       61 agaaagttaa ttttcattaa atgaatcatc tttattgata aacataattc caaagaccac
      121 tgccaaaata aaaaaacata ttagtattga tttttccacg aaaatttttg taaaaaaagt
      181 tcctaaaata gctccagata taaagaatat aataatagca taatactgta aactcttttt
      241 tatcataaaa atattcttag tatgtatata atgaaataaa gtttctgtag cacttcttaa
      301 atttccagta cacattgtcg ttgcaaaact attcccattt acttttctaa aactctctac
      361 ttgcattgaa caaataaaag aaatagcaac atttacaaac atattaaatc ttcctctagg
      421 aataaatgaa acaataagta ttgtaatcat ttcaattata atgataacct gacgccaatg
      481 aatctttgtc ttattatttg taaaatactt tcttatcttc tcggctataa atacacctaa
      541 cataaatgcc aaaataggaa tcaaataata aaatgattcc tttacattac cttctgctat
      601 attaagccca aataaaacta tatttccagt ttgtgcatta gcaaagactt tacctcttac
      661 cacataagta taaatatcca aaaatcctcc tgttattgcc aacaaagaac ataaaaagaa
      721 tgattcagac atctgactct tgcttgtcat atataaacct ctcgctttct aatgatagat
      781 acaaaatcaa aatcatttta gattct
//
LOCUS       DAELSY010000333          773 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.337, whole genome shotgun sequence.
ACCESSION   DAELSY010000333 DAELSY010000000
VERSION     DAELSY010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.337"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          260 a           91 c           80 g          342 t
ORIGIN      
        1 atatttttgg aaattatata tattataaca attatagcaa atttttaggg tataattttt
       61 aaaattatat tctactatca aaagatatga ataaatgatt gtatatatta tatcaatttt
      121 tatatttcat agtccatttt ttcatcacat tttcgcttgt caagaataat atctttaaga
      181 gtgaagcgtt tgatgtaaat ctggactttg tttttgagac atttttgaaa tatttattaa
      241 atccttcgat tatacttgta atatatatta tagatattac ttattttagt aaaactaaag
      301 gctgatgcta caacattcca gttatttcca aacgttttaa tttctgaagt atactgttta
      361 cattattttt aatctatttt atctaaagtc gataaggctt tttattcatt aactgaccta
      421 tatacagatt taaaatcact tattaattct tttgagtcct tgtatgaaac atatttaaag
      481 tatttcttat ctaatgtatt aaacattttt gtattacaga atgaggataa cttgtagcta
      541 ttgtctcttt gaaataagtc atctgtattt actcctaaaa ctacttatat ctgtagattt
      601 tataagttct ccatcttata taatttcttt gattaattgt tttcttaaat tattcataaa
      661 aaaatctccg ttacgatatt tgattatatt taaatcattg ccataaagaa gacttttatt
      721 aactttgaaa aacaaattat tttacattac tggtgattag ttgctacttt ctt
//
LOCUS       DAELSY010000334          730 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.338, whole genome shotgun sequence.
ACCESSION   DAELSY010000334 DAELSY010000000
VERSION     DAELSY010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.338"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          304 a           72 c           74 g          280 t
ORIGIN      
        1 agacagtttt acatatttca actgtctcct aagaggggta tagttcacaa ctaagagtgg
       61 ctgggaactt aatataaagt tccagaaaag taaatcttca aaataattta ttgaaggaaa
      121 catcactcaa agttaagtaa aatatgtttc cttgctttta ttatatcaca aattaaaaat
      181 attaacacaa aattttgtga atatatactt attattgttc taaaaaaata tgtaaataat
      241 tgatttgatt tcatagaatt attgttgtaa tatttagtta aatatacaca atctcaatta
      301 aagaagataa gtattataaa ttcatatcaa aaaaatacat aaacttttaa gttataattt
      361 tataaaaagt cttttagtat cattatttca agaggaatta acatatgaaa aaataacttc
      421 attagaaaag tggataaaaa actggtaaga agatgactaa agattacata tactattact
      481 taaattaaaa atttaatata atttataatg tatacaattt attataaaaa atttaatttt
      541 aattttctat ttttgatact ataatatata atcttgataa tttaattaaa aatctagatt
      601 ttacatacca aactggtttc ttatttcgct atctttactt atgaactcat atattcctat
      661 aaaggttatt ttagaaaaag ttggttattt aaacattaat accacaattt ctatagattg
      721 tttgttgaat
//
LOCUS       DAELSY010000335          703 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.339, whole genome shotgun sequence.
ACCESSION   DAELSY010000335 DAELSY010000000
VERSION     DAELSY010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.339"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            349..633
                     /locus_tag="KPG09_003899"
     CDS             349..633
                     /locus_tag="KPG09_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968403.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGRICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
BASE COUNT          305 a           48 c           97 g          253 t
ORIGIN      
        1 caactaaaat atacagaaag ttttatatta actatatgga atgtaaagtc tagttttttc
       61 tctttagaaa aaataaaaac aactgtttta tattaactat atggaatgta aattaaaatt
      121 gtgttttctc tttcatacac ccacttcatt agttttatat taattatatg taatataaat
      181 gttttttctt attaaataaa gattgagtca ctttatattt aataatacat attctaaatt
      241 gattttgttt ttatataaga gaatttgaat aatatggaaa aagatgatta ataaattatt
      301 aattagggta taaaatgata taaatagaat aaataagggg tggatgaaat gcttgtatat
      361 aataaaagtt tttatcctaa tgacatattt ccaagattag atttttcaaa aataaaaaaa
      421 cagttaaaat tgatagataa tgacctgtca gattttggaa gaatatgtat aatagaaaaa
      481 gaacattata cgataagtgt aaacagtata ggtgaaataa atgtgtatta tgatttagag
      541 tacgaaaata aggtgtatag aatagtttat gagattgaaa agttatttaa atctcaagtt
      601 ggaaggttta gtatatctac atacagaaat tgataattaa aaaagtagaa attaaaaaac
      661 ttaatactaa agatatagat aaatatataa ggtaacagct aga
//
LOCUS       DAELSY010000336          700 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.340, whole genome shotgun sequence.
ACCESSION   DAELSY010000336 DAELSY010000000
VERSION     DAELSY010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.340"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..423
                     /locus_tag="KPG09_003900"
     CDS             <1..423
                     /locus_tag="KPG09_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0968404.1"
                     /translation="TISKKYKMRSVNPLKAWVKEYNLHKEIKETPKGGLFMETRKTTL
                     DERVEIVKDCIEHDFNYNLIAKKYFVSYSQVRRWTLKYKENGIVGLEDRRGKAKKEDE
                     LTQLDEIKLELKIVKAEKHKLQLENAFLKKLKNIERGW"
     gene            417..>700
                     /locus_tag="KPG09_003901"
     CDS             417..>700
                     /locus_tag="KPG09_003901"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0968405.1"
                     /translation="MVITLSKNENKYKAIQSLFNEKKASLMQLCEIAKVNRSGYYKWL
                     NRDKSNLELENIKLATLITNIYEEKKGVFCSLRMTLQLNREYKLNVNHKRV"
BASE COUNT          307 a           78 c          126 g          189 t
ORIGIN      
        1 actatctcaa aaaaatataa aatgaggtct gttaatccac taaaagcttg ggttaaggag
       61 tataatcttc ataaggagat aaaagaaacc ccgaagggag gactctttat ggagactaga
      121 aaaacaacat tagatgaaag ggttgaaatt gttaaagatt gtattgagca tgattttaat
      181 tacaatttaa tagcaaaaaa atactttgtt tcatacagcc aagttagaag atggactctt
      241 aaatataaag aaaatggtat agttggttta gaagaccgaa gaggtaaagc aaaaaaagaa
      301 gatgagttaa ctcaattaga tgaaataaag ttggaattga aaattgtaaa agcagaaaaa
      361 cacaaattac aactggagaa tgcattctta aaaaagttaa aaaacataga aagagggtgg
      421 taattacact atccaagaat gaaaataaat ataaagctat tcaatcctta ttcaatgaaa
      481 aaaaagcttc gcttatgcaa ttatgtgaaa tcgctaaagt aaatcgttct ggatactata
      541 aatggctcaa tcgagataaa agtaatttag aacttgaaaa cattaaatta gcaactttga
      601 taactaatat atatgaagaa aagaagggag ttttttgctc tttaaggatg accttgcagt
      661 taaacagaga gtataaactt aatgtcaatc acaagagagt
//
LOCUS       DAELSY010000337          687 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.341, whole genome shotgun sequence.
ACCESSION   DAELSY010000337 DAELSY010000000
VERSION     DAELSY010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.341"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(75..488)
                     /locus_tag="KPG09_003902"
     CDS             complement(75..488)
                     /locus_tag="KPG09_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0968406.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKTEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT          248 a           91 c           70 g          278 t
ORIGIN      
        1 tgatataaaa acataaagtc tgtagacgaa gagcagaagt tttaagttaa aaatataaaa
       61 tatgattttt attattatag tatttttcta taataatatc tctttccagt tagaggataa
      121 ttttcaagtg aaaatacttt atggtatcca aatttctcat aaaatttagg tgcttgaaaa
      181 ctgaatgtat ctaaaaaaac atatttacaa cctcgttctt tagcaatttt ttcagtctta
      241 tataaaagtt gacttccagt acctttatat ctaagcttct cactaaccca taagtatttt
      301 atagaaagcc aattaccatg agtaacacct ataaaacctg caacaatttt acctgcttta
      361 tctcgaatat atattcctaa atccactggg tttttatctt caagtctagc aagattatat
      421 tttaataatc cttgaaaaat agtatcttca tcttgttttt ctattttttc tgtaatttct
      481 aattccatat ttgaactttc tccttgtata atcttttgta tattggtatt ttttaataaa
      541 atcagtagaa agaatatagt ttaacattat tctaataggc atttatgcta atgtcaaact
      601 aataatactt attcttaatt ttatattgtt gatattaaat tttttaaaat aaaaatgtag
      661 tttaagttat ataaaaagaa atcatca
//
LOCUS       DAELSY010000338          637 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.342, whole genome shotgun sequence.
ACCESSION   DAELSY010000338 DAELSY010000000
VERSION     DAELSY010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.342"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            364..516
                     /locus_tag="KPG09_003903"
                     /pseudo
     CDS             364..516
                     /locus_tag="KPG09_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010982.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
BASE COUNT          251 a           60 c           88 g          238 t
ORIGIN      
        1 ttaaaagttt taagatttca tatctagagt acctaacatt acatgttagt ttcttgttac
       61 ttatctaatt actttatatt tcgtagttat atataaaatt actataaaaa atgaaatata
      121 ttaaacaatc aactaaaaaa tgaaatatta ataaaaaaaa ttcaaattat aaatattaaa
      181 tgaatataat taaaacaaat atataaattt aagtataaca ctcacatttt aatgtattgt
      241 gaaatttttg ttgaacttta tgtaccataa cttataaaat ttatgtcatg ttatatttga
      301 ataagtaata aaattgtaga tgcaaattaa tctacaaaat ttatatgaaa tggagttgat
      361 gctatgacag aattaatgat tataagtggg ttagtactat tgatttgtat aacttctagc
      421 aaggtattgt ataaatttgg agtaccaata ttattgattt ttatcttact agggatgtta
      481 tttgggtctg atggaatggt aggaatctat tttgattaaa aaatagctac gcacacttgt
      541 gactttagtc atgagttagt agctaaaata gtcagcatat agggaaacct atatgtagag
      601 ataggataga acctatccaa tactacttga attgctg
//
LOCUS       DAELSY010000339          611 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.343, whole genome shotgun sequence.
ACCESSION   DAELSY010000339 DAELSY010000000
VERSION     DAELSY010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.343"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            380..574
                     /locus_tag="KPG09_003904"
     CDS             380..574
                     /locus_tag="KPG09_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0968407.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYEM
                     TDAYRFETLCSDEKIRFKRI"
BASE COUNT          230 a           61 c           68 g          252 t
ORIGIN      
        1 atatagtgat tatttttata tataatctta atcaatgaga agtaagaagc tatagataat
       61 gcttatatca tttatggctt cttatttttt atgtaatact caaattttta tagtattctc
      121 ttattattaa acagcttctg cctagaatat tccatatata tatgacttct ttcataaaac
      181 ttgaactgcc cataactaaa gtcacaagta tacataacta ttttttaatc aattataacc
      241 aatatttatt ttatttatct tcattttaat tcttcattca ataatgtaat aagattattt
      301 tttatttgga tactattata tcatctaaat atgttattat atatttggat attttcatat
      361 taataggagg gattctacaa tgataaatta tatggtagaa attatatata gcgaaaaaat
      421 aagcgaagta gaaaacatgt tgaaaattga aaatatacaa tatgaattta cttctcgtga
      481 tggctttata ttttttggtt tttatgagat gacagatgca tatagatttg agactttatg
      541 ttcagatgaa aaaattcgat ttaaaagaat ataataaata aaaaaagtac atatttctaa
      601 actatttgtt t
//
LOCUS       DAELSY010000340          594 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.344, whole genome shotgun sequence.
ACCESSION   DAELSY010000340 DAELSY010000000
VERSION     DAELSY010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.344"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            47..415
                     /locus_tag="KPG09_003905"
     CDS             47..415
                     /locus_tag="KPG09_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0968408.1"
                     /translation="MKKIPKSELLVMNFIWDKGIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
BASE COUNT          244 a           51 c           89 g          210 t
ORIGIN      
        1 attagacatt tgtctaatat aatcaagtta aaggtgagtg attatattga aaaaaatacc
       61 aaaatccgag ttgttagtta tgaatttcat atgggataaa ggcatagata aaataccagc
      121 cagagaagta gctaattata tggaaatttc tcatggatgg acaaaaggta ctactggtaa
      181 agtactttct agattaagtg ataaaggttt tttagaattt gaaaaagatg gtagaaatac
      241 tttatacact attaaagtta aacatgagga atatctaaaa tttgaaacaa aggatttttt
      301 agagcttata cataaaaatt ctattcatag ctttatatca tcattaggtt gtggaaatga
      361 aatatcagat gatgacataa aagaacttga acaatggcta aaaaaaagaa aataggagac
      421 gtattttatg gaattattta tagaagttat taaatttaat atttacataa gtctactaat
      481 tctttttttc attttattta ctaaaaagat aagcaaatat acttttaagt ttaattattt
      541 tatatgtctt ttaatagtag tcagaatgat gtttatatct aaaataaatt taaa
//
LOCUS       DAELSY010000341          573 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.345, whole genome shotgun sequence.
ACCESSION   DAELSY010000341 DAELSY010000000
VERSION     DAELSY010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.345"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            150..548
                     /locus_tag="KPG09_003906"
     CDS             150..548
                     /locus_tag="KPG09_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968409.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRRNARRHNIL
                     TKLVLSIIVIFLVIVLVVLIFIKRKKKKFY"
BASE COUNT          278 a           38 c           72 g          185 t
ORIGIN      
        1 ctttgaaaaa ttttaaaaaa agtaaaagtt atgataataa aaataatatt aaagataata
       61 agatatataa gaaacttgaa aaaatttgta atttttagtt atatatatat tataatgaac
      121 tagttaaaaa tttttatgag ggggaaaata tgaataagaa gaaaaaattg attgggatag
      181 ctatatcttt tctacttata atcagtatta taaatccatt acaagtagaa gcagaagcta
      241 ttattgagtc tagtgtatac atttcttcaa taaaagagaa ttatacatat gataatacta
      301 aatttaaaac tattaaatct aaaagtagac ataaaagtag gaaatataat aaaagtaaag
      361 taaagttgaa aaaacgaagt ataagaacta aaaagtatgg atatcaaact ataaataaat
      421 ataaaagacg aaatgcaaga agacataata tcctaacaaa attagtattg tccataatag
      481 tcatattttt ggttatagtt ttagtagtgc taatatttat taaaagaaag aagaaaaaat
      541 tttactaaaa ataattaaaa gaatatcatt ttt
//
LOCUS       DAELSY010000342          570 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.346, whole genome shotgun sequence.
ACCESSION   DAELSY010000342 DAELSY010000000
VERSION     DAELSY010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.346"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            15..>570
                     /locus_tag="KPG09_003907"
     CDS             15..>570
                     /locus_tag="KPG09_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0968410.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILV"
BASE COUNT          213 a           56 c          109 g          192 t
ORIGIN      
        1 tagggggtgg cataatgaat gacaacatag tagaagttaa aaatataaat atgaacttct
       61 acactaagga aggcgagctt gaagtattaa aggatgtcaa ttttaacttg aaagaaggag
      121 aaatattaac tttgcttggt ccttctggaa gtggtaaatc tacaatatta aatattctta
      181 ctaatctttt aaaaccgact agtggtgata taaagattac tggaaatatt ggatatatgt
      241 ttcaaaaaga taatttgctt gaatggagaa atataatgga taatataact attggattgg
      301 aaattcaagg caaaaaagat aaaaaatctt tagatagagt tgaaagcctt ttaaagactt
      361 atggattatg ggattttaga agtatgtatc caaaagaatt atctggaggt atgagacaaa
      421 gagttgcgct tattagaaca ctttctgtaa atccagatat tctactttta gatgaacctt
      481 tttcagctct tgattatcag actagattat tagtttgtga tgatgtttat agtattatta
      541 agaatgagaa taagtctact attttggtta
//
LOCUS       DAELSY010000343          557 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.347, whole genome shotgun sequence.
ACCESSION   DAELSY010000343 DAELSY010000000
VERSION     DAELSY010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.347"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          220 a           43 c           72 g          222 t
ORIGIN      
        1 taagaatgta taaggtggta aatttatata aaaaagaata tgagttgtaa aatattaaat
       61 aatattgtac taattggaat aattttaact ttattataga ttattttaat gccattagta
      121 ctgatggtgt attttaaaat tggtataagc attacaaata cgaatatgcc aactgtaata
      181 tgtaatataa gtagctatat acatatgtgc tgtcccatat attgtatcat tgtttatatt
      241 aaaaagatta tgtaaattgg tatcaatcaa taaatcattt tcagaagaaa tacctaaaaa
      301 cttaaaaaat acctatatgt gtatttagtt agattggaga tttttacatt taattttata
      361 gtgacaatat tatattattg ttatgatgtc tatttatatg caattactat aatatcagaa
      421 cttatagtat catttgtttc attatcaata aggtttttta ctttgttttg tcaattttat
      481 taaaaaaagt tattgatata aaagataaga aggataaaac tatatgacag atttcaaaca
      541 taagagtgga tatttat
//
LOCUS       DAELSY010000344          468 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.350, whole genome shotgun sequence.
ACCESSION   DAELSY010000344 DAELSY010000000
VERSION     DAELSY010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.350"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          152 a           65 c           31 g          220 t
ORIGIN      
        1 tttttaattc aatttaaatt ttaaatattt tgtgtttatt atataatctg aaaaaaaagc
       61 agtcaatagc atttttaatt tttttaattt tcaaaattat gattcttaaa aaatcgtttt
      121 ccttttatca taacacctat taacgttaat tcttgtagta ttttgtattt attttgctaa
      181 attgacagtt aactttattc aatttttatt ctactcacgt agatttacct cttgtctttt
      241 agtcttaaaa ccattttttc tgaaaattcc ttattatgat ttatcaactt atttttcttc
      301 atagcaatta ctttctaact tccaacttac ttaaactttg cagttgctag tattcacgtt
      361 tacatactta tatgtcataa ttaactaaaa attcttaata tatctctcaa tgaaaacaat
      421 ttactcattt atttaataca tttatataat catatgtctt aaattttg
//
LOCUS       DAELSY010000345          455 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.351, whole genome shotgun sequence.
ACCESSION   DAELSY010000345 DAELSY010000000
VERSION     DAELSY010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.351"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      192..282
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          191 a           42 c           62 g          160 t
ORIGIN      
        1 atagaataaa gatgagattt gtctaatatt tttataaatt aaaaatattt tcaaagagtt
       61 tattataaaa ttgtagacta tagtgtttat aatctttgta attattggta actaaattta
      121 aaaaataaaa tatttaatta tacacaaaaa agatatttaa aaaatttgtt atttattgta
      181 tacttaagtt aaaactaaat aggcaaatct agagaaatct agtgacgcaa agctataggg
      241 gctaaggttt gtatacacaa actatgtcag ccagttgcca aaaagagttc taggtatatt
      301 gtatacgtag gaagagtcta ggatgactgc ctttttggtg gtcattttgt tatggaaaaa
      361 atcacattat gtagtaaaaa acacctataa taacatatta taccaaatat aatttaaaat
      421 aaagataaat tatctataaa agttactatt ttaca
//
LOCUS       DAELSY010000346          454 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.352, whole genome shotgun sequence.
ACCESSION   DAELSY010000346 DAELSY010000000
VERSION     DAELSY010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.352"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..372)
                     /locus_tag="KPG09_003908"
     rRNA            complement(<1..372)
                     /locus_tag="KPG09_003908"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          120 a          109 c           73 g          152 t
ORIGIN      
        1 aggactatta ccttctgtgg tgggtctttc caaacctctt caattacgat acctcttcgt
       61 tatgatatgt ccgcaacccc aataaagaaa actttattgg tttgggctaa tccgcgttcg
      121 ctcgccgcta cttacggaat cgaatttctt tctcttcctt caggtactta gatgtttcag
      181 ttccctgagt tcccctcatt aagctatgta ttcacttaat gatacttaga cattactcta
      241 agtgagtttc ctcattcgga aatcttcgga tcaaagttta cgtgcaactc cccgaagctt
      301 atcgcagctt atcacgtcct tcatcggctc ctagtgccaa ggcatccgcc ctgcaccctt
      361 aataacttga ccagttaaaa aggtttgata gattagaagc tatcaacttc gataatttta
      421 aaaagttatc agctttatat attacaaatt agat
//
LOCUS       DAELSY010000347          420 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.354, whole genome shotgun sequence.
ACCESSION   DAELSY010000347 DAELSY010000000
VERSION     DAELSY010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.354"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>420
                     /locus_tag="KPG09_003909"
     rRNA            <1..>420
                     /locus_tag="KPG09_003909"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          106 a           97 c          130 g           87 t
ORIGIN      
        1 cgcctcccaa aaggtaacgg aggcgctcaa aggttctctc agtacggtcg gaaatcgtac
       61 gtagagtgta aaggcaaaag agagcttgat tgcaagacat acaggtcgag caaggatgaa
      121 aatcggactt agtgatccgg tggttctgcg tggaagggcc atcgctcaac ggataaaagc
      181 taccctgggg ataacaggct tatctccccc aagagtccac atcgacgggg aggtttggca
      241 cctcgatgtc ggctcatcac atcctggggc tgtagtaggt cccaagggtt gggctgttcg
      301 cccattaaag tggtacgcga gctgggttca gaacgtcgtg agacagttcg gtccctatcc
      361 gtcgcaggcg taggaaattt gagaagacct gtccttagta cgagaggacc gggatggacg
//
LOCUS       DAELSY010000348          373 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.355, whole genome shotgun sequence.
ACCESSION   DAELSY010000348 DAELSY010000000
VERSION     DAELSY010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.355"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            177..293
                     /gene="rrf"
                     /locus_tag="KPG09_003910"
     rRNA            177..293
                     /gene="rrf"
                     /locus_tag="KPG09_003910"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            300..371
                     /locus_tag="KPG09_003911"
     tRNA            300..371
                     /locus_tag="KPG09_003911"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:332..334,aa:Asn,seq:gtt)
BASE COUNT          118 a           61 c           80 g          114 t
ORIGIN      
        1 tttgatgtta ttcattaaga tatatgtgca gtttttagag tgttaactct atgtaaagta
       61 ctaacttcaa ttgatgaagt atatatcgta aaaacaatta ctatttagta cttcaaaact
      121 attgacattt aaaaataaag atgttaatat aatacttgtc ttataaatag gcaatatgtg
      181 gttataatag caaagaggat acacctgttc ccattccgaa cacagaagtt aagctcttta
      241 gcgctgatgg tacttggtgg gaaactgcct gggagagtag gacgtagcca cgtgaatatt
      301 ccaaggtagc tcaatggtgg agcaaccggc tgttaaccgg taggctgtgg gttcgagccc
      361 cacccttgga gcc
//
LOCUS       DAELSY010000349          346 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.356, whole genome shotgun sequence.
ACCESSION   DAELSY010000349 DAELSY010000000
VERSION     DAELSY010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.356"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          151 a           17 c           54 g          124 t
ORIGIN      
        1 acataatatg ataaaataaa ttcaaagagt taaattgtag gtaaatagta ttttaattat
       61 ttattaaaat atagattatg tatatgccaa atttaaaaag agacataaag acattataca
      121 aatatattat tggggtgata ttttataaga aaagaagtga tgcagtaaat taattgagca
      181 atttaaaaat gatttaatga tagtgttgat gaatgtttaa aaagaaattt tagaaaaacg
      241 tatattgctg tttgtgttgg aggtgatgaa tttgtataat ttatagggta taaatgatta
      301 aactacttaa gataaataca tatcactgta aaatattgat aatcat
//
LOCUS       DAELSY010000350          336 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.357, whole genome shotgun sequence.
ACCESSION   DAELSY010000350 DAELSY010000000
VERSION     DAELSY010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.357"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>336
                     /locus_tag="KPG09_003912"
     CDS             <1..>336
                     /locus_tag="KPG09_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0968411.1"
                     /translation="LCEEYGWRSFLQSILFDRFGKKIGIVMVGTCWSLWHLPLQFTLY
                     SPQTPIIGAIAHLIYGIGLSIFLGYVYMKTKNIWFCSIMHVLINSLGLIFNDSEIIIN
                     YHSIIQRLI"
BASE COUNT          109 a           36 c           55 g          136 t
ORIGIN      
        1 tttatgtgaa gagtatggtt ggagatcttt tttacaaagt attttatttg atagatttgg
       61 caaaaaaata ggtattgtaa tggtaggcac ttgttggagt ttatggcact tacctttaca
      121 atttacacta tatagtcctc aaactccaat aattggagct atagcccatt tgatatatgg
      181 tattggactt tctatatttt taggttatgt ttatatgaaa actaagaaca tatggttttg
      241 ctctataatg catgttctaa ttaatagctt aggattaatt ttcaatgatt ctgaaataat
      301 tattaattac catagtatta tacaaagatt gatatt
//
LOCUS       DAELSY010000351          320 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.denovo.359, whole genome shotgun sequence.
ACCESSION   DAELSY010000351 DAELSY010000000
VERSION     DAELSY010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.denovo.359"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>320
                     /locus_tag="KPG09_003913"
     CDS             <1..>320
                     /locus_tag="KPG09_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBG0968412.1"
                     /translation="SNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFNDYN
                     EGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSNIL
                     RYIF"
BASE COUNT          134 a           24 c           46 g          116 t
ORIGIN      
        1 ttctaactat tatagagaga tatcttattc atgtactgat gataatgatg taaacaaagt
       61 tttgaaaagg ataaacaaac ttgaaatatt tgtatcatat ccgtttatat tagaattatt
      121 taatgattat aatgaaggta taataaataa aaaagtactt gtagaatccc ttaatttagt
      181 agaatgtttt atattaagaa gaatagtatg tgatgtacct ccgaatgcac tgaataaagt
      241 atttatgaac ttaggaaggg aaattaaaaa atttagtgat tataaagaaa actattctaa
      301 tatattaaga tatattttta
//
LOCUS       DAELSY010000352         6363 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.guided.192, whole genome shotgun sequence.
ACCESSION   DAELSY010000352 DAELSY010000000
VERSION     DAELSY010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.guided.192"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            35..736
                     /gene="vanR"
                     /locus_tag="KPG09_003914"
     CDS             35..736
                     /gene="vanR"
                     /locus_tag="KPG09_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBG0968413.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KDLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            786..1868
                     /locus_tag="KPG09_003915"
     CDS             786..1868
                     /locus_tag="KPG09_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0968414.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            2129..3229
                     /gene="vanG"
                     /locus_tag="KPG09_003916"
     CDS             2129..3229
                     /gene="vanG"
                     /locus_tag="KPG09_003916"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBG0968415.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            3226..4032
                     /locus_tag="KPG09_003917"
     CDS             3226..4032
                     /locus_tag="KPG09_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0968416.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            4050..6188
                     /gene="vanT"
                     /locus_tag="KPG09_003918"
     CDS             4050..6188
                     /gene="vanT"
                     /locus_tag="KPG09_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG0968417.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
BASE COUNT         2336 a          721 c         1111 g         2195 t
ORIGIN      
        1 tgacctcgat aataaggaga gtgaaaaata atctatgaac gaaaatattt tgattgtgga
       61 tgatgaaaaa gaaatagcag atttaattga agtatattta aaaaatgaag gttttacagt
      121 atacaagttt tatacaggta aggaagctct agaatgtatt gaatctaaag atttggatat
      181 ggctatttta gatattatgc ttcctgatat tgatggattt catatttgcc agaaaattcg
      241 agaaaaatac tattatccta tcattatgtt aacagctaaa attgaagata tagacaagat
      301 aatgggttta accataggtg ctgatgatta tataacaaag ccatttaacc ctttggaagt
      361 tgttgcaaga gtaaagacac aattaagaag atatatgcga tataataatt cttatgaaca
      421 acagtcgata attgttaatg agtatgatat taaaggattg attatcaata aagaaacaca
      481 taaatgtagt ttatttggga aagaagtagc attaacaccg attgaatttt ctattctttg
      541 gtatttatgt gaacatcaag gaaaagtggt tccttctgaa gaattgtttg aagctgtatg
      601 gggagaaaaa tatcttgata ataataacac agttatggct catatagggc gattacgtga
      661 aaaaattcat gaaccttcta aaaacccgaa atttataaaa actgtatggg gagtaggtta
      721 tacaattgaa aagtaaaaat attaaagtca atcctgactt tactcaagtg agaaataaaa
      781 tttttatgag aacagcttta atggcattta tagccattat tttcatatgg attttatatt
      841 cttttatatt gtatggtaag ttttctatct ggatagtatc attctttgaa aatgcttttc
      901 gtatggaata taaagctgca ttgaatttat atcagcagat tttccgtaat agcatggagc
      961 tcttcatgat actagctatg gtgattgtgt tttttataat attccgtgtt tatttaaatt
     1021 ggtttacaaa atattttagt gaaataaatc aaggtattga ctctcttatc aaagaagatg
     1081 ttggagaagt ggctttatct ccagagttat tggcaataga aaagaaaata aactcaatta
     1141 aacatatact tgaacaacgt aaatttgaga cacaaatggc agaacaacgt aagaatgaac
     1201 ttattgttta tcttgcacat gatttaaaaa caccacttac atcagttata gggtatctga
     1261 cactacttcg agatgaaagt cagatttctg aagaactgcg aaaaaaatat ttatctattt
     1321 cgttggataa ggcagagcgt cttgaagatt taattaatga attctttgaa attacaaggt
     1381 ttaatttatc aaatatatca cttgaataca gtacagtaag cttaactcgt atgcttgaac
     1441 aactaacata tgagtttaaa cctactttga tgagtaagaa tttaaaatat acactgaatg
     1501 ttacaccaaa tatgatgata aggtgtgatg ttaataaaat gcaacgtgta tttgataact
     1561 tattacgaaa tgctgtttat tatagttttg aagatactac tattgaaata acagctgtac
     1621 aggaggatga ttatataaag cttaaattta taaatcatag caatacgatt ccagaggaaa
     1681 aacttgagcg tatttttgaa caattttaca ggttggatac atctcgtaat tcaaacagtg
     1741 gtagagctgg gttgggactt gctatagcta aagaaatagt ggagcttcat aacgggacaa
     1801 taacagctca tagcgagaac aatataatta cctttaaggt tattattccg attgtaggaa
     1861 atttgtaaga atttaagtat aaaaaaatag gaaattcttt aggataaata aataaagagc
     1921 actcttgtta ttgatataat tactgtatca aaacaagagt gcttttattt tgatacagta
     1981 attatatcaa tagaaaaatg tgaaaatata ttatgtggaa cagatgcaat tgtaagtgaa
     2041 ggttgattgg atttgaaata gaaaaagtat tttaacatgt ttctgtagat tgtataaaaa
     2101 taaataaatc aacaaaggag aatcaaagat gaataataaa aaaattgcag ttatatttgg
     2161 tggaaactca acagaatatg atgtatcatt acaatcagct tttgcagtac ttgagaattt
     2221 gaatacggaa aaatatgata ttataccaat tggaattaca agggagggta attggtatca
     2281 ttatattgga gattataata atatccagaa taacacttgg tttgaaaggg atactgatat
     2341 aattccagtt attgtttcgc agaaccgtgt caaaaatggt attattgaat ttttaattcc
     2401 tgatgctgtt tctcagaatc atattccaaa tagtgctatt gaatcatgtg aaaatgatat
     2461 taaatacact aaacttgatt ttgtatttcc tgtattgcat ggaaaaaatg gagaagatgg
     2521 aacagtgcag gggttatttg aattggcagg tatacaggtt attggatgtg gtactctttc
     2581 ttcagcatta tgtatggaca aagacaaatc acataaactt gttcattcag aaggtattaa
     2641 agtaccaaaa tcaatattac taaaatactc agataaattt aatgtggctg aactgtcaaa
     2701 agacttaact tatccattat ttataaaacc tgtgcgtgca ggttcatcat ttggtattag
     2761 taaaatttat aaaaaagaag agttacaagt agcagtagac ttggcttttg aacatgataa
     2821 tgaagtaata attgaggaaa atattgatgg ttttgaggta gggtgtgcag ttttaggaaa
     2881 cgatgactta cttgttggaa gagtagatga aatagaattg tcaaaaggat tttttgatta
     2941 tacagaaaaa tatacattga aaagttcaaa aattcatatg ccagctagaa ttgatttgga
     3001 ttgtgagaaa agaatacaag agacagcaaa aattatttat agaactcttg gatgttctgg
     3061 atttgctaga gtagatatgt ttttaactcc atctggtgaa attattttta atgaagtcaa
     3121 tacaattcct ggctttactt ctcatagtcg ttatccgaat atgatgaaag gtattggact
     3181 gtcttttgaa gaaatattgg ataagataat tgaggtgtac ataaaatgat gaataatatt
     3241 ttattagata aaaataaaga agataaaatg acaaatacta tcttattgac taaagataaa
     3301 gtttatgatg ggaatctaat tctagtaaat gcttttttac cagttaaaac atctgaagat
     3361 atagatttaa ttcctgtaga tacaaggttt ccaagtattc ttatgaaacg tgaagctaca
     3421 aatattttac aaaatatttt aaaaagtata tgtggaataa atgaaatagt acctgtaagt
     3481 ggatatcgta ctgcagagga acaacaagat atatattcaa gctctctgcg tgacaatgga
     3541 aaagacttta ctaaaaagtt tgtagcactt ccaaatcaca gtgaacatca gacagggctt
     3601 gctattgatt tagggttgaa gaaagaggtt atagatttta tctgccctga atttccttat
     3661 aatggtattt gtaatgagtt ccgtaaggca gcactacatt atggttttat agagcgttat
     3721 cagtgtaaaa aagaagaaat aacaggaata tctcaagaac catggcattt tcgttatgtc
     3781 ggatatccac attctgagat tatcaataaa atgaatctcg cccttgaaga atatatagaa
     3841 tatattaaga gattttcgta tacaaattgt ttaaaaatag aagataaaga aaaaattata
     3901 gaaatctttt atgtgcctat tcatactaaa gaagaagtga gaatctctat acctgaacaa
     3961 tgtgtatatc aaatatcggg taacaataag gatggtttta ttgtgacatt ttggagaaat
     4021 agaaaggact aagaaatgga ggaacaagaa tgattaggaa taaatcttat gcagggcttg
     4081 attattttag gattatagct gcattactta taattgcaat tcatacatct ccattattat
     4141 catatagtaa aatggctgac tttgtgttaa ctagtataat tgctcgtatt gctgtgccat
     4201 tcttttttat gctatcaggc ttttttctaa tttcaacata taattataat tgtaataaac
     4261 tgaaatcttt tctaaaaaat gtattattta tttacgggat agctattgta atttatattc
     4321 ccttaaatat ttacactgga tatttttcca tgaataattt agtgcccaat attattaagg
     4381 acattgtatt tgatgggact ttatatcatt tatggtatct acctgcatca attattggtg
     4441 caagtattgc atactttctt gtaaaaaaac taggtttaaa gtactcatta gtaatcacta
     4501 ttgttattta tgtaattggt atgtttggtg atagttatta tggtttttta gaaaaaatac
     4561 cactattaaa gaatttatat gggtatattt ttgaagtatc agattataca cgaaatggta
     4621 tattttttgc acctgtattt ttcatacttg gtggaataat atctaataaa tctaattata
     4681 tgtcaactaa aaatagcttg attggatttc ttatatcatt cttacttatg ttatgtgagg
     4741 gaatgttttt acataaacta ggggtacagc gtcatgatag catgtatatt atgttattgc
     4801 catgtatgta tttcttattt acagccttaa catttttaaa aggttctcga gctaggttat
     4861 tgcgaacatc atcattaatt atttatatta tacatcctat gatgattgtt gttattcgta
     4921 tgttttctaa aatattcgga atacaaactg tcttggttga caatagcctt attcattata
     4981 tagtggtaag tatagcatct tttatagctt ctataattat tatattgatt cttaaacctc
     5041 taaaaaaaga cttgaaagga gaacataagg taaataccaa tagagcttgg attgaaattg
     5101 atttaaataa tttggaacat aatgtaaaga tgatacaaaa agctatgcca gaagattgtg
     5161 aattgatggc agttgtaaaa gctaatgcct atggtcatag tgcttttgaa gtagcgactt
     5221 gtgcaaacca aataggtgta aaagcatttg cagtagcaac aatagatgag gggattgatt
     5281 tacgtcgata tggaatacta ggagaaatat tgattcttgg atatactaac ccaataaggg
     5341 caaaggaact tcataaatac aatcttacac agagtgttat tgaccttaat catgctatct
     5401 ctttaaacaa acagaaatac aacataaaat cacacattaa aattgataca ggtatgcaca
     5461 gactgggttt tgatgttaat gatgtgaggt caattttaaa ggcatttaaa ttaaaatact
     5521 tagatatatg tggcatattt acacacttgt gtgtttcaga tagtctgaaa gatgaagatg
     5581 tagactttac aaataaacaa attgaatgtt tttataagtt aattgatttt ctgttaaaaa
     5641 aaggtataaa aattcctaaa gtacatattc aaagtagtta tggttttttt aattatccca
     5701 atcttaagtg taattatgta agaattggaa ttgcattata cgggatatta agttctccaa
     5761 atgatacgac taaattgcaa cttgatttac gacctgtttt atccttaaaa tcacaagtaa
     5821 ttcttattag aaaaatacaa aaagatgata gttttggata tggaagagtc tttattgcaa
     5881 gcagagatag cgtaattgct atattatcta ttggctatgc cgatggtata cctagaaatt
     5941 tatcctgtgg aaaaagttac gtaataataa atggttgtag agcagcaata gtgggtcgaa
     6001 tttgcatgga ccaacttgct gttgatatta cagatatacc taatgtagaa gttggtaata
     6061 cggcaataat aataggaaga gataatttat ctgaattatc tgcttctgaa gttgcaaata
     6121 actcatgtag tatatcgaat gaattattga gcagagtagg aagaaggtta aatgttataa
     6181 agaagtaact caattaaata gtaatatttg ttgtactgtg taatttattt tactctgact
     6241 agacattttt agtaatatta tgtctaatat atatttttta taacttacat gacaatttaa
     6301 gttataaaaa ggtgatgttt cagattttta ttttgaaaca tcaccttttt tgttataata
     6361 tat
//
LOCUS       DAELSY010000353         4804 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.guided.220, whole genome shotgun sequence.
ACCESSION   DAELSY010000353 DAELSY010000000
VERSION     DAELSY010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.guided.220"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..223
                     /locus_tag="KPG09_003919"
     CDS             <1..223
                     /locus_tag="KPG09_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0968418.1"
                     /translation="MKKLNRNLDVVKISGNRNLVYFFFGAELQSIDGIVIFKDLKHVK
                     PKLDFINRSIQAPRLEYITNNVYYFKGEL"
     gene            251..1189
                     /gene="blaCDD"
                     /locus_tag="KPG09_003920"
     CDS             251..1189
                     /gene="blaCDD"
                     /locus_tag="KPG09_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG0968419.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            1533..2216
                     /locus_tag="KPG09_003921"
     CDS             1533..2216
                     /locus_tag="KPG09_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0968420.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2213..>4804
                     /locus_tag="KPG09_003922"
     CDS             2213..>4804
                     /locus_tag="KPG09_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0968421.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAQKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDRTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKMS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKTVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNTRSRRQEFAMLRSVGLSPE
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIAS"
BASE COUNT         1853 a          552 c          840 g         1559 t
ORIGIN      
        1 aatgaaaaaa ctaaacagaa atttagatgt agttaaaatt tctgggaata gaaatttagt
       61 ctattttttc tttggagctg aattacaatc tattgatggt attgtgattt ttaaagattt
      121 aaaacatgtt aaaccaaagt tagattttat aaatagaagt attcaagctc ctagattgga
      181 gtatattaca aataatgtat attattttaa aggtgaacta taaacttatg tagaggagat
      241 ggtataaagt atgaaaagaa aaaataattt tatttggata gttattcttc tagttgttgt
      301 agctatggga gtatattatg taggaagaaa gcataacaac acaaaacaaa aacatgataa
      361 gaatcatata aactctgaat taaataaatc caaagttgaa aacaatgata aaaacaaaaa
      421 agaaaaaatt aatatggttg actatagtga ctgttttgaa ggtattagtg gaggagctat
      481 tttttataat actaaaaata aagaatataa tatatataac aaagaattaa ttgaaacaag
      541 aaggtcacca tgttctactt ttaaaatagt ttcaacttta attggtttag aaaaaggggt
      601 aataaactca aaagaatcag ttatgggata tgatggtaca gagtatccaa ataaaaattg
      661 gaataaaaat cttagtttag aagaggcatt taaggagtct tgtgtttggt actacaaaaa
      721 attgatagat aaagttgatg ctaaatctgt ccaaaacatt ttagatgatt taaagtatgg
      781 gaattgcgat attagcgaat gggaaggaga tttaaaaaat ggaaagggac atttaaatgg
      841 attttggtta gaatcttcat tacaaatatc tccaaaagaa caagtgcaaa caatggcaaa
      901 aatatttgag ggagatacaa actttaaaaa agagcatatt aatattttaa gagatattat
      961 gaaaatagat gtaaatgata aaaacataaa tgtatatgga aaaactggaa caggatttga
     1021 tgaaaaaaat aaacgtgtgg atgcatggtt tgttggtatg ctagagcgtg aaggtgacac
     1081 ctattatttt gctattaaat cagatgattc caataaagaa attacagggc caaaggtaaa
     1141 ggaaattgca attaatataa ttaagaaata ttatagtgta agggaataaa cttaatttta
     1201 tttgtaaata ttatttacct atagtctgta atatttatta aataatttta ctaaattgag
     1261 tctatataga gcaaaactcc tttagtacat aaaatacttg ctattgatta ttaaattgaa
     1321 tacctataga tttaaagtta atacctttta aagactatgt aatatattaa aagtataaat
     1381 aaaatatatt gcatagtttt tgttttatat tataatttta aataatgtgc aaaaactaaa
     1441 caatattttt aatagtaatt tatatttgta ttcatgtcac tataatgtca ttttaggttg
     1501 ctaaaattta accataaagg agatgattgt atatggaaat attaaaatgt gaaaatctca
     1561 caaaaatata tggttcaaat caaaccagag tgacagccct taataatgta aatttatcag
     1621 ttcaaaaagg tgattttgta tccattgtag gagcatctgg ttctggcaaa tctacattgt
     1681 tacatctgct tgggggagta gatagaccta catctggaaa gatttatgtt gaagatacag
     1741 aaatatcaag tttaaaggaa gaagctcttg cagtttttag acgtagaaaa gtaggtctta
     1801 tttatcaatt ttataatctt atacctaccc ttgatgttag aaaaaatata ttattaccca
     1861 tgttacttga taaaagaaag gtggatgagg atagattctc tgagattgta tctatattgg
     1921 gattatctga tagattaaac catcttccaa gtcaactttc aggtggacaa caacaaaggg
     1981 tatctatagc tagaagttta atctatagac cagctatttt attagcagat gagccaactg
     2041 gtaatcttga tagaaaaaat tcagaagaaa ttgtagactt gttaaactta tcaaataaac
     2101 gatttaatca gacaatactt cttattactc atgatgagaa aatagcacta gaggcaaatc
     2161 gtattgtaac tatggaagat ggagtgattg tctctgaaaa ggtggtgaag aaatgaatat
     2221 tcttcaagaa tacaccttag actttgtacg acgcaataaa cgtagtagta tagctataat
     2281 gttagctata ttacttacta caaccatgat gtcttctttg tgtggactgt tgtataccat
     2341 gtggacagat tttataagac tttcaattga gaaagaaggt aactggcatg gtgaattgtt
     2401 tgactatact tatggaagtg acttaccatt aattgaaaat ttttcttcag ttgatgatgt
     2461 aatggtaaaa ggacaatggt atgtaggcaa aattaatgat gagaaaagag attatataat
     2521 ttatagaaat gctacatcag agtattggaa ttctatgcca gaaaaggata ctataataga
     2581 gggtcgtcga ccaaataatg ctaatgaaat tgcattatca aaacaatatt ttgagaataa
     2641 tccaagtgta aaaattgggg ataaaattac tcttccaata ggagatagaa tcctaaatgg
     2701 aaagcctctt gaacccatag ctcaaaaatc gaaaggggaa agttttaaaa aaatttcaga
     2761 gactacattg acaatagtag gaaaggtaga tgtttccact tcatcagtaa tacctgcata
     2821 tacagctctt ggatatcttg atagaacaag tataaaacct aatgatagta taactgtgta
     2881 cttgcgattt aaaaatataa gagacacata caaggaactt ccaaaacttg ctaaatcatt
     2941 ggggtggaag actaatgaat atggaaaata caatcttaaa tataatagta attatctttt
     3001 aaaaatgtta gtcttttccc cagaacagaa agcatctatg agcagtatat caaaattttc
     3061 aactcctatt atgtatctta caatagcaat ttttacagta gctgtgtttg taatggtgat
     3121 ttacaacgcg ttttcactat ctgcaaatgc tagacttact caattaggga tattatctag
     3181 tgttggtgca tcaccaaaac agattaaaat gtctgtagtg tttgaaggtt ttttgcttac
     3241 aattatacca ttgccaatag gtttattttt aggatggtta ttgtgtaata ggcttatagt
     3301 ttatattaat tcagttaatt atcatactga tgttccagaa gtggtattta cttatggtat
     3361 tcctgctttt ctgccagcag ttttacttac aatagtaaca gtctggattt ctgcacttat
     3421 accagcacga aaagtatcaa agatatctcc tatagaagca atacgacaag gagatagtgt
     3481 taaaatcaaa aagtcgcata aatattcatt aggaaagata tttggaatag aaggagaact
     3541 tgcatcaaac gcactacgtg ctcgtaaaaa atcttatcgt actgcaacaa tatctttaac
     3601 attatctttt ttattactta catgtttttt gcatataatt gcaaatcaag aaggagcaaa
     3661 agctgtttat ggaagtgata attataagga ccaaagagat atagcagttt atttggaaaa
     3721 tagtgaacct gtagagtctg ctttcattga aaaattagat aatacagaag gtgtaaaaag
     3781 ttcactatat tatcaaaaaa tgtctgcttc cacttggctg actgaaaaag atgcatcaaa
     3841 agaacttgcg aaaaatggag gatttaaaag tatagtttca tctaaaaaat actctcctat
     3901 agaacgtgat ggcaagtttc gtataatttc aaatataatt gggcttgatg ataagagttt
     3961 tacagaatat tgtaggcaaa tttctgtaga cccaaagtta ttttatgata caaaaaatcc
     4021 aacctcaatt gtatataata aggaggaaga tattaatcgt agtacacgta gaaataaagt
     4081 atatatagat tatttaaatc tttcaatagg agataacatt aaattaaatg aaaaagtata
     4141 tgacgaagat aaaggggatt atacttacaa catgaaagtg ggagctatta caagcacact
     4201 tcctaaaaca gtagatacta gttcaaaata tacattaatg caaatagtgc caatgtctat
     4261 ggtgcaggaa atatctaaaa attatgatga agtaagtagg ttacgttcaa ataggctgat
     4321 aggagtattt aaagtaaata atcgtgagga cattcctaga gtaagagaaa aattagagtc
     4381 catttataat aaagactatg gttcaggaga ttatagtatt gaggatgttt tagaaagtga
     4441 aaaacaaaag gctagtggaa taaagaccat gaatttaata gtaggcttta ttacaggttt
     4501 attggctatg attggattat caaatgtact tgctacagtg tcaggaaata ctagaagtag
     4561 acgacaagag tttgctatgt tgcgttctgt tggattatct ccagaaggca taaaaaagat
     4621 gcttgttttg gaaggtttgt tcttaggaat tacaccatta ctacttagta ttcctgtgca
     4681 aataggaata gtgtatgctt ttctacgtat aaatgaaata tattttatag agtatttacc
     4741 atttgcacct atatctacaa taataggttt tactatattg atacttttta ttgtaatagc
     4801 atca
//
LOCUS       DAELSY010000354         1359 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.guided.307, whole genome shotgun sequence.
ACCESSION   DAELSY010000354 DAELSY010000000
VERSION     DAELSY010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.guided.307"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            488..1120
                     /locus_tag="KPG09_003923"
     CDS             488..1120
                     /locus_tag="KPG09_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG0968422.1"
                     /translation="MTIPNSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVSPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT          554 a          145 c          178 g          482 t
ORIGIN      
        1 cactacttat aaagagattg aatattaatg aatctaaatc atttgcatta cttcagagtt
       61 ttgactaaag ttgaacacca tacaaaagta gctactcaat tgtttatacc ccaaccaagc
      121 ttaagtaatg ttatatagat tcttgaaaaa gaatttggta tctttttatt tgaaaaacaa
      181 ggtagaaatg tttccctcac taaatacgga agtttttttc taacctatgt tgataaagca
      241 ttaggttaat tagaaaaagt ttacatataa aatatattaa attgttgcaa gttttattgt
      301 tttgatatac aatcaactaa acatataatc tttataaaat actatacagt taaaagtctg
      361 ttttaaaaaa aaatgtataa attataaatc aaaatataga tactatcaag gagtgattct
      421 gtgaagagat gtattgtagt agtggaatag tcaaaaacat aatatatatt aaaggagaga
      481 tttaaatatg actattccaa attcaaataa ggtatatcca agaagtaatg attatcaaac
      541 tgtatatctt aaaaatgtaa taacaaggga aaatatagaa gttggagatt acacaatata
      601 taatgacttt tataatgacc caagagattt tgagaaaaac aatgtattat atcattaccc
      661 tataaacaat gataaattaa taattggtaa gttttgctca atagcatgta atgcaaaatt
      721 tctaatgact agtggaaatc actcaatgaa atcaaaatcc acatatactt ttcccatctt
      781 ttatgaagaa tggaaactcg atatcaacca tataacggac gcttgggaca ataagggtaa
      841 cattataatt ggaaatgatg tgtggatagg ttatgattct ataattatgt caggtgtaaa
      901 tattggtgat ggtgcaatta ttggaacaag agcagtagtt actaatgacg tctcaccgta
      961 ttctattgtt ggaggagtac ctgcaaaaat tataaaaaag agatttaata atgatacaat
     1021 ttcaaaatta ttagaaatta aatggtggga ttggccatat aaaaaaattc aatcaaacat
     1081 taagcatatt caatcaggaa atattgataa attagtttaa tattttataa tttatttata
     1141 ttaaacttta ctttaataat ccttttataa gagctttttc aaaatttgta tattatttta
     1201 atatatataa atttaataca acattaaata aaataaaaag taaagttact taataaaaag
     1261 ttactttact ttttattatt taaattcctg tttttattat aataggtaaa attctattaa
     1321 actagttcac attttcatta ttattgatat attatatat
//
LOCUS       DAELSY010000355          874 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017014927
            SAMN15907545-rid12313013.guided.334, whole genome shotgun sequence.
ACCESSION   DAELSY010000355 DAELSY010000000
VERSION     DAELSY010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907545
            Sequence Read Archive: SRR12520810
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050662.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050662.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 09:26:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,924
            CDSs (total)                      :: 3,842
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 25 of 51
            Pseudo Genes (incomplete)         :: 25 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907545-rid12313013.guided.334"
                     /isolate="M2017014927"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Olmsted County"
                     /collection_date="2017-07-11"
                     /collected_by="CDC, Emerging Infections Program"
     gene            163..861
                     /gene="vanR"
                     /locus_tag="KPG09_003924"
     CDS             163..861
                     /gene="vanR"
                     /locus_tag="KPG09_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBG0968423.1"
                     /translation="MSGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQIRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
BASE COUNT          331 a          105 c          132 g          306 t
ORIGIN      
        1 atctatatat attcattttt atctttaata tatgttaaat aataaaatag ttacataata
       61 agattaagaa tttcttaata aattctaatg taaaaaatca tataaaaaaa ttaaatttag
      121 tataatatat ataaatatat atagaaagga tgtttaaagt ctatgagtgg aaaaatttta
      181 gttgtagatg acgaacgtga tatagctgat ttaatagaag tctaccttac aaatgaaaac
      241 tatattgttt ataaatttta ttctggtctt gatgcactaa actgcattaa tttaaatgaa
      301 tttgaccttg caattttaga tgttatgttg ccaggagtaa atggattttc tttatgccaa
      361 aaaatccgag aaaaatatac ttatccaatc attatgttaa cagctaagga tgaagaaaca
      421 gacaagatta caggcttaac tttaggtgca gatgattatg ttactaaacc tttccgacct
      481 ttggaacttg ttgctagagt gaaggcacag attagaagat ataagaaata cagtggaagt
      541 ttacaatctg gtgaagaact ttcacatatt ttttcttttt taggtctgga attgaatacc
      601 caaacatatg aatgccattt aaatggtaaa gctctttcac ttactccaac agagttttct
      661 attctacatg tattattgaa aaataaaggc tctgtggtaa attcagaaaa tctttttcat
      721 gctatctgga aagatgaata ttacaataaa agtaacaata ctataacagt tcatattcgc
      781 catcttcgtg aaaaattagg tgatactgta gatgaagcaa aatatataaa aacaatttgg
      841 ggtgttggtt acaagatttg acacctattt ttct
//
